LOCUS       DATMCV010000001         6428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_797414, whole genome shotgun sequence.
ACCESSION   DATMCV010000001 DATMCV010000000
VERSION     DATMCV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797414"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..315
                     /locus_tag="VNU03_00005"
     CDS             142..315
                     /locus_tag="VNU03_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72853.1"
                     /translation="MSVPDASLWSVMLLGLFFGVQHATDPDHVVAVATIVARPAASGR
                     ARWWERSGAWATP"
     gene            282..923
                     /locus_tag="VNU03_00010"
     CDS             282..923
                     /locus_tag="VNU03_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity nickel-transport family protein"
                     /protein_id="HWP72854.1"
                     /translation="MGAFWGVGHTVTVTAVGIAIVVFNLALTPRVALSLEMLVAIMLI
                     ALGVARIVWVFRDSDPVAVSHLGEPHAHGDGPALHSHAHTHAAGVHRHPHAHPPVGLL
                     RALQTVGPAQALRSALVGLVHGLAGSAAVALLVLSTIRGTTQAIAYLLVFGAGTILGM
                     TAITGVLSLPFTIRAPRLERSRRALTLATGALSLGFGLYMVGLLLGRIPGGHP"
     gene            complement(930..1376)
                     /locus_tag="VNU03_00015"
     CDS             complement(930..1376)
                     /locus_tag="VNU03_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HWP72855.1"
                     /translation="MLSAVVSSLHLLALAIGLPAVFLRGRALKGPLDADGLRRLLAAD
                     NVWGIAALLWIVTGLLRAFAGLEKGSDFYLHSPLFWTKMALFLLVLLLEIRPMITFIR
                     WRIKLGRGETVDTSVAPALYTHNHIELAIVVVMVFVASLMARGVGY"
     gene            1379..1924
                     /locus_tag="VNU03_00020"
     CDS             1379..1924
                     /locus_tag="VNU03_00020"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP72856.1"
                     /translation="MKPSTAIALAGLLLGAAGCATYPGGADVPFLVTPPEIVDQMLRL
                     AKVGPADTVYDLGSGDGRLVIAAVRDFGARAAIGVEIDPTLVARSREYARRAGVADRV
                     TFIEQDFFRADLAPASVVTLYLTREVNMRLAPRLRSELRPGTRVVSFNFDMGDWEPRA
                     MIRVDVNGRTTPVYLWVVPER"
     gene            complement(1935..2861)
                     /locus_tag="VNU03_00025"
     CDS             complement(1935..2861)
                     /locus_tag="VNU03_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWP72857.1"
                     /translation="MAGKASRAAFGEALIELGAGDQRIVTLDADLSKSTMTGAFAKKF
                     PDRAFDLGIAESGMIGVAAGLALTGHVPFACSFACFLAGRFETIRVSVAYTEAPVKLV
                     GTHVGVAIGEDGYTQMGLEDVACLRSLPNIPIVQPADEIETRQAVAYAVGHPGPIYLR
                     LTRQNLEPVHEASYRFQFGKADVLRPGHDVTVLATGGPVWFALEAAKTLAAEGIQAEI
                     INVATIKPLDEETILRSAGRTGHVVTVEDHSIHGGLGSAVAELLSEVMPTPLKRIGVT
                     TFGESGDFKGLYAKHGLDAAGIAGTVRKFLAR"
     gene            complement(2861..3757)
                     /locus_tag="VNU03_00030"
     CDS             complement(2861..3757)
                     /locus_tag="VNU03_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWP72858.1"
                     /translation="MANPDVAKLETIAREGRVQILRMLTHAGSGHPGGSLSVIDILTD
                     LYFNRLRLDPARPQWEDRDRFILSKGHCVPAQYYCMARTGFFPMDRLITLRKLGSPLQ
                     GHPDRVALPGIEAATGSLGQGISVAVGMALGLKLAGKGARVYCVLGDGETQEGQVWES
                     LMSAPKLGAPNHHLDNLCVILDYNGIQLDGFVKKILDLEPVTDKLKAFGWPVLEVNGH
                     DIAQVDKALDQAEATKGAPTFIVAHTVKGKGVSFMENDPEWHGKAPKPEEAVRAIREI
                     VGEPPPAWAAPIVAELSALGKK"
     gene            complement(3757..5304)
                     /locus_tag="VNU03_00035"
     CDS             complement(3757..5304)
                     /locus_tag="VNU03_00035"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWP72859.1"
                     /translation="MDLRRAVGEALARLSAEPGVREVEVFAALNRALLTRLNYTSHIA
                     CNGVEEPKSTEMTGLGVHAVFDGPDGRPLLGFGSEPSDLTPDGARRALAKARQAAVHD
                     PAFRSLPRPTGEPRALTDYHDPRLMDISDAELVEAGWKVVSGGLRAFMASSRLADLVP
                     DEAGLKRLGLILGGDVSIRQERIAIASTAMPEPQADDATFMSASTTAMVEARDAKGSG
                     WSVCTRLEHFTDEAGSEAADNAVAAIDGARAESGDYTVVFGPQPVADLCNNLIVPSCQ
                     AGSFFTSSTPFLGRLGKTVAAAGLGIYDHGAMPGLAGSRGITCEGLPTGRTDLIKDGV
                     LVGLLSHWYDTQRLLHDPDARQKLGVDPASATAALRPRNGFRYDGAGRSFETTPGTSA
                     TNVVVEGRDAVPLDRLLARVRDGLYVGRIWYTYPINGLRAGDFTCTVVGDSFIIRDGR
                     ITAPLKANTLRINDNLTRLLGAVVGMTKDAKGTLVWGADEIVYAPSIAVTGVHVDAIA
                     GFMEGLD"
     gene            complement(5312..>6428)
                     /locus_tag="VNU03_00040"
     CDS             complement(5312..>6428)
                     /locus_tag="VNU03_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWP72860.1"
                     /translation="RAVALDEMVRFTTDVSRQVGADAAAVSYNYISTLTQLSRELFAS
                     SEGALIDQSFALTQGMAYVVASGPDNSQEVYDVLGHQRGWEILTRGVSEPLIEFPTFP
                     EFALAIARDAAAVCRAPVLEAPAGEVTVVTDPHYNTLLSHEIVGHPVELDRGLKMETA
                     YAGRSWLLRGLDDTQLGRRIASPLVTAFSDPSLPGYGHYAYDHEGTPARRVVHIDRGI
                     FTGFMNSRQTAAIFGGDPNGHWKATDAQLVPLVRMSSTVFAGGEHDPDELIREIDHGY
                     YLVGHRTPSIAESRENFRISARKVYEIRRGEIGPLFRDGGIMADSRDYLMHVDGVGRD
                     FRLYPIPNCGKGQPMQTRKLGNGGPTLRSRAVLTGGA"
BASE COUNT          874 a         2292 c         2300 g          962 t
ORIGIN      
        1 ctacggcttc ccgcctcgct tcgcgcatcc gttcgcgatc ggcttcgcgc tctccctgct
       61 ctacgcggtg ctcgcccact gggcgctcgc cgccgcggtg acccgctcgc gccgcaccgg
      121 gctcctgctc aagctctcgg agtgagcgtg cccgacgcct cgctctggtc ggtgatgctg
      181 ctgggcctgt tcttcggcgt ccagcacgcg accgatcccg accacgtggt ggcggtcgcc
      241 accatcgtgg cccgacccgc cgcttcgggg cgggcgcgct ggtgggagcg ttctggggcg
      301 tgggccacac cgtgaccgtc accgcggtgg gcatcgccat cgtcgtcttc aacctggccc
      361 tgaccccgcg ggtggccctg tcgctcgaga tgctggtggc gatcatgctg atcgcgctcg
      421 gcgtcgcccg catcgtgtgg gtgttccgcg actccgatcc ggtggcggtc tcccacctgg
      481 gcgagcccca cgcgcacggc gacggccccg cgctccacag ccacgcgcac acccacgcgg
      541 ccggggtgca ccgccatccc cacgcccacc cgccggtcgg tctcctgcgc gcgctccaga
      601 cggtcgggcc ggcccaggcc ctgcgctcgg ccctggtggg cctcgtgcac ggcctggccg
      661 ggagcgcggc ggtcgccctg ctcgtgctct cgaccatccg gggcaccacg caggcgatcg
      721 cctacctgct ggtcttcggg gccgggacca tcctcggcat gaccgcgatc accggcgtgc
      781 tctcgctgcc gttcacgatc cgcgcgcccc ggctggagcg ctcgcgccgg gccctgaccc
      841 tggccaccgg ggcgctctcg ctcggcttcg ggctctacat ggtcggcctg ctgctcggcc
      901 gcatcccggg cgggcacccg tgagagaggc tagtagccga ctccgcgagc catgaggctg
      961 gccacgaaga ccatcaccac cacgatggcc agctcgatgt ggttgtgggt gtagagcgcg
     1021 ggggccacgc tcgtgtcgac ggtctccccg cggccgagct tgatgcgcca ccggatgaac
     1081 gtgatcatcg ggcggatctc gagcaggagc acgaggagga acagcgccat cttcgtccag
     1141 aagagcggcg agtgcaggta gaagtcggag cccttctcga ggcccgcgaa ggcacggagg
     1201 aggccggtga cgatccacag cagcgccgcg atgccccaca cgttgtcggc ggccagcagc
     1261 cggcgcagcc cgtcggcgtc gagcggcccc ttgagcgcgc gcccgcgcag gaagaccgcg
     1321 ggcagcccga tggccagcgc cagcaggtgc agggaagaga ccacggcgga gagcatggat
     1381 gaagcctagc acggcgatcg ccctcgccgg cctcctgctg ggcgccgccg gctgcgcgac
     1441 gtaccccggc ggggccgacg tgccgttcct ggtgacgccc cccgagatcg tcgaccagat
     1501 gcttcggctc gccaaggtgg ggccggccga cacggtctac gatctggggt cgggcgacgg
     1561 ccgcctcgtg atcgcggcgg tgcgggactt cggcgcccgg gccgcgatcg gggtcgagat
     1621 cgatcccacc ctggtggccc ggagccgcga gtacgcgcgc cgggccggcg tcgccgatcg
     1681 ggtgaccttc atcgagcagg acttcttccg ggcggacctc gcgcccgcca gcgtggtgac
     1741 gctctacctc acgcgcgagg tgaacatgcg gctcgccccg aggctccgat ccgagctacg
     1801 gccgggcacg cgggtggtct cgttcaactt cgacatgggc gactgggagc ctcgcgcgat
     1861 gatccgggtg gacgtgaacg gacgcaccac cccggtctac ctctgggtgg tgccggagcg
     1921 ctgaggcgtc gcggctagcg ggcgaggaac ttgcggaccg tgcccgcgat cccggccgcg
     1981 tccaggccgt gcttggcgta gagccccttg aagtcgcccg actccccgaa ggtcgtgacc
     2041 ccgatgcgct tgagcggggt cggcatcacc tccgagagca gctcggccac cgcgctcccg
     2101 agaccgccgt ggatgctgtg gtcctccacc gtcaccacgt ggccggtgcg gcccgcggag
     2161 cgcaggatcg tctcctcgtc caggggcttg atggtggcca cgttgatgat ctcggcctgg
     2221 atgccctcgg cggcgagcgt cttggccgcc tcgagcgcga accagaccgg gccgccggtg
     2281 gccagcaccg tgacgtcgtg gcccgggcgc agcacgtcgg ccttgccgaa ctggaagcgg
     2341 tacgacgcct cgtggaccgg ctcgagattc tggcgggtga gacggaggta gatcgggccc
     2401 ggatgcccga ccgcgtaggc caccgcctgc cgcgtctcga tctcgtcggc gggctgcacg
     2461 atcgggatgt tcggcaggga gcggaggcag gcgacgtcct ccaggcccat ctgggtgtat
     2521 ccgtcctccc cgatcgcgac gcccacgtgc gttccgacca gcttgaccgg ggcctcggtg
     2581 taggcgaccg acacccggat cgtctcgaag cgccccgcca ggaagcaggc gaacgagcac
     2641 gcgaagggca cgtggccggt gagggccagg cccgcggcca cgccgatcat gcccgactcc
     2701 gcgatgccca ggtcgaacgc gcggtccggg aacttcttgg cgaaggcgcc ggtcatggtg
     2761 gacttcgaga gatcggcgtc gagggtgacg atccgctggt cgccggcgcc gagctcgatc
     2821 agcgcttctc cgaacgcggc gcgcgaggcc ttgcccgcca tcacttcttc cccagggcgg
     2881 agagctccgc gacgatcggc gcggcccacg cgggcggagg ctcgcccacg atctcccgga
     2941 tcgcgcgcac cgcctcctcg ggcttcgggg ccttgccgtg ccactccgga tcgttctcca
     3001 tgaacgagac tcccttgccc ttgacggtgt gcgccacgat gaacgtgggc gcgcccttgg
     3061 tggcctccgc ctggtcgagg gccttgtcca cctgggcgat gtcgtgcccg ttgacctcga
     3121 gcaccggcca gccgaaggcc ttgagcttgt cggtcaccgg ctcgagatcg aggatcttct
     3181 tcacgaagcc gtcgagctgg atgccgttgt agtcgaggat gacgcagagg ttatccaggt
     3241 ggtgattcgg ggccccgagc ttgggcgccg acatcagcga ctcccacacc tgaccctcct
     3301 gggtctcccc gtcgcccagc acgcagtaga cgcgcgcgcc cttccccgcg agcttgagcc
     3361 ccagcgccat gcccaccgcc accgagatgc cctggccgag ggagccggtg gccgcctcga
     3421 tcccgggcag ggccacgcgg tccggatggc cctggagcgg gctcccgagc ttgcgtaggg
     3481 tgatcagccg gtccatcggg aagaagccgg tgcgggccat gcagtagtac tgggcgggga
     3541 cgcagtggcc cttcgagagg atgaaccgat cgcggtcctc ccactgcggc cgggccggat
     3601 cgaggcgcag gcgattgaag tagagatcgg tgaggatgtc gatcaccgac agcgagccgc
     3661 cgggatgccc ggacccggcg tgggtcagca tccggaggat ctgcacccgt ccctcgcgcg
     3721 cgatggtctc gagcttcgcc acgtccggat tcgccatcag tccagtccct ccatgaatcc
     3781 tgcgattgcg tccacgtgca cgccggtcac cgcgatgctc ggcgcgtaca cgatctcgtc
     3841 cgctccccac acgagcgtgc ccttggcgtc cttcgtcatg ccgaccaccg ccccgagcag
     3901 ccgcgtcagg ttgtcgttga tgcggagcgt gttcgccttg agcggcgccg tgatccggcc
     3961 gtcgcggatg ataaaggaat cccccaccac cgtacaagtg aagtctcccg ctcgcaaccc
     4021 gttgatcggg tacgtgtacc agatgcggcc gacgtagagc ccgtcgcgca cgcgcgcgag
     4081 cagccgatcg agaggaaccg cgtcgcgacc ctcgaccacc acgttggtcg ccgacgtgcc
     4141 gggcgtggtc tcgaaggagc ggcccgctcc gtcgtagcgg aagccattgc gcggccggag
     4201 cgcggccgtc gcggaggcgg gatccacccc gagcttctgt cgtgcgtcgg gatcgtgcag
     4261 gagccgctgg gtgtcgtacc agtggctgag gaggcccacc agcacgccgt ccttgatcag
     4321 gtccgtgcgc ccggtcggca ggccctcgca cgtgatgccg cgcgagcccg cgagccccgg
     4381 catggccccg tggtcgtaga taccgagccc cgccgcggcc accgtctttc cgaggcggcc
     4441 caggaacggg gtgctcgagg tgaagaacga ccccgcctgg cacgacggca cgatcaggtt
     4501 gttgcagaga tccgccaccg gctgggggcc gaagaccacc gtgtagtcgc ccgactccgc
     4561 gcgggccccg tcgatcgcgg ccaccgcgtt gtcggccgcc tcgctgcccg cctcgtcggt
     4621 gaagtgctcg aggcgcgtgc acacgctcca ccccgagccc ttggcatccc gcgcctccac
     4681 catggcggtg gtggaggcgg acatgaaggt ggcgtcgtcg gcctgcggct ccggcatcgc
     4741 ggtggaggca atcgcgatgc gctcctgccg gatgctgacg tcgccgccca ggatgaggcc
     4801 caggcgcttc agccccgcct cgtcggggac gagatccgcg agccgcgagg aggccatgaa
     4861 ggcgcgcagg ccgccggaga cgaccttcca gcccgcctcg accagctccg cgtcgctgat
     4921 gtccatcagg cgcgggtcgt ggtagtccgt cagcgcgcgg ggctccccgg tgggccgcgg
     4981 cagcgagcgg aacgcggggt cgtgcaccgc ggcctggcgg gccttggcga gcgcgcgccg
     5041 cgcgccgtcg ggggtgagat cgctcggctc cgatccgaag cccaggagcg gccggccgtc
     5101 cgggccgtcg aagaccgcgt gcaccccgag cccggtcatc tcggtggact tcggctcctc
     5161 caccccgttg cacgcgatgt gggaggtgta gttgaggcgg gtcagcagcg ctcggttcag
     5221 cgccgcgaac acctcgacct cgcggacccc cggctcggcg gagaggcgcg cgagggcctc
     5281 cccgaccgcc cgtcggagat ccacgggcgt atcaggcccc gccggtgagg acggcgcggc
     5341 tccgcagggt gggcccgccg ttgccgagct tgcgggtctg catcggctgg cccttgccgc
     5401 agttcgggat cgggtagagc cggaagtcgc ggcccacccc gtcgacgtgc atcaggtagt
     5461 cgcggctgtc cgccatgatc ccgccgtcgc ggaagagcgg cccgatctcg ccccgccgga
     5521 tctcgtagac cttgcgcgcg gagatccgaa agttctcccg gctctccgcg atggacgggg
     5581 tgcggtgtcc caccaggtag tagccgtggt cgatctcccg gatcagctcg tccgggtcgt
     5641 gctcgccgcc cgcgaacacg gtggacgaca tgcggaccag cgggaccagc tgggcgtcgg
     5701 tggccttcca gtgcccgttg ggatcgcccc cgaagatcgc cgcggtctgc cggctgttca
     5761 tgaagccggt gaagatcccg cggtcgatgt ggaccacccg gcgggccggg gtgccctcgt
     5821 ggtcgtaggc gtagtggccg tagccgggca gggagggatc cgagaacgcg gtgaccaggg
     5881 gggaggcgat ccgccgcccg agctgggtgt cgtcgaggcc gcgcagcagc cacgatcgtc
     5941 ccgcgtacgc cgtctccatc ttgagcccgc gatcgagctc gaccggatgg ccgacgatct
     6001 cgtgggagag gagcgtgttg tagtgcggat cggtgaccac cgtgacctcg ccggcgggcg
     6061 cctccagcac cggggcccgg cacacggccg ccgcgtcgcg ggcgatggcg agggcgaact
     6121 cggggaaggt ggggaactcg atcagcggct cggagacccc gcgggtcagg atctcccagc
     6181 cccgctggtg cccgagcacg tcgtagacct cctggctgtt gtcgggcccg ctcgccacca
     6241 cgtaggccat gccctgggtg agcgcgaacg actggtcgat gagcgcgccc tccgacgagg
     6301 cgaacagctc ccggctcagc tgggtgaggg tggagatgta gttgtaggag acggcggcgg
     6361 cgtcggcgcc cacctggcgg gacacgtcgg tggtgaaccg caccatctcg tcgagcgcga
     6421 ccgcccgc
//
LOCUS       DATMCV010000002         3066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_531754, whole genome shotgun sequence.
ACCESSION   DATMCV010000002 DATMCV010000000
VERSION     DATMCV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531754"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VNU03_00045"
     CDS             complement(<1..1221)
                     /locus_tag="VNU03_00045"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP72861.1"
                     /translation="MSHHGHLDARTLHARLSHPVIDADGHWLEYGPVMREEFRRIGGE
                     AAVEGLAAASQRVPNSLKMTVAERTRRRVGQEAFWSSPCENVLDRATAMMPRLMYERL
                     PDLGIDFAVVYPTAGLGYHRMQDTRMRRAICRAYNVFAADQFRGLEDRVIPAAIIPMY
                     TPEEAIEEIEFAATQLGYKVIMVGGMMRRPVPALAEERPDAASLVEWYDVVGLDSPHD
                     YDPVWRKCRELKLAPTFHNGARSILLRNSPSNFCYNHIGHFASAGHAVAKALFFGGVT
                     RRFPDLNFAFLEGGVGWACMLYADLLGHWEKRNGQAIQSTHPSKLDRAKLLELAQKYG
                     RPEVVEAVRRGEGLEGDSNSTLTGGVEDVDDYFRCRIEQKQDIRDLFVPRFYFGCEAD
                     DPANAWAFNTKANPM"
     gene            complement(1259..1996)
                     /locus_tag="VNU03_00050"
     CDS             complement(1259..1996)
                     /locus_tag="VNU03_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWP72862.1"
                     /translation="MADMSPAVRADLAPTGKLRAAINYGNFILATKDKATGESRGVAV
                     DLTQEVGKGLGVPVEIVPYDSVAVMGDAAPSGVWDIAFLGSDPAREKIMSFTAAYLEI
                     EATYLVPGGSPLRTAAEVDREGVRVAAPARANYELYLSRNLKRAQLVSTAGADAAFEL
                     LASGKVDALAGLTQGLLGLAPKLPGARLVEGRFMGVQQSIAVPKGKDAGLRYLRQVVE
                     DAKRSGLVARAIERTGAKGVSVAPPAP"
     gene            2238..2987
                     /locus_tag="VNU03_00055"
     CDS             2238..2987
                     /locus_tag="VNU03_00055"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016953716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWP72863.1"
                     /translation="MRKLEDKVAIVTGASKGIGASIAKHLAAEGAAVVVNYASSREGA
                     DRVVAEIARAGGRALAIQADLAKPADVDRLFAETAKAFGRLDVLVNNAGIYEFRPLED
                     ITPEHFHKYFDVNVLGVLLASRAAVKHFGPAGGSIINISSGATRLTPANSAVYSATKA
                     AVEAITGVLAKELGPRRIRVNAVLPGVVLTEGVRAGGFDEGDFRKEMEARTPLGRIGQ
                     PEDIAPAVVFLAAEDSAWITGESLLVAGGLR"
BASE COUNT          451 a         1047 c         1122 g          446 t
ORIGIN      
        1 catgggattg gccttcgtgt tgaaggccca cgcgttagcc gggtcgtcgg cctcgcagcc
       61 gaaatagaag cgcggcacga agaggtcgcg gatgtcctgc ttctgctcga tccggcagcg
      121 gaagtagtcg tcgacgtcct ccaccccgcc ggtgagggtg gagttggagt cgccctccag
      181 gccctcgccc cgccgcaccg cctcgacgac ctcggggcgg ccgtacttct gcgccagctc
      241 cagcagcttc gcgcggtcga gcttgctcgg gtgcgtcgac tggatggcct gcccgttgcg
      301 cttctcccag tggccgagga ggtcggcgta gagcatgcag gcccagccca ctccgccctc
      361 caggaaggcg aagttgaggt ccgggaaccg ccgggtgacg ccgccgaaga agagggcctt
      421 ggccaccgcg tggcccgcgg aggcgaagtg gccgatgtgg ttgtagcaga agttcgacgg
      481 cgagttgcgc agcaggatcg agcgcgcccc gttgtggaag gtcggcgcca gcttgagctc
      541 gcggcacttg cgccacaccg ggtcgtagtc gtgggggctg tcgaggccga ccacgtcgta
      601 ccactcgacc aggctcgccg cgtcgggccg ctcttccgcc agggccggca ccggccgccg
      661 catcatgccg ccgaccatga tgaccttgta gccgagctgg gtggcggcga actcgatctc
      721 ctcgatggcc tcctcggggg tgtacatcgg gatgatcgcg gcggggatga cgcggtcctc
      781 gagcccgcgg aactggtccg ccgcgaagac gttgtaggcg cggcagatgg cccggcgcat
      841 ccgggtgtcc tgcatgcggt ggtagccgag cccggcggtc gggtagacca ccgcgaaatc
      901 gatgccgagg tcgggcagcc gctcgtacat cagccgcggc atcatggcgg tcgcgcggtc
      961 gagcacgttc tcgcacggcg aggaccagaa cgcctcctgc ccgacccgcc gccgggtccg
     1021 ctcggccacc gtcatcttga gcgagttggg cacgcgctgg ctcgccgcgg ccaggccctc
     1081 gacggccgcc tcgccaccga tgcggcggaa ctcctcgcgc atcaccggcc cgtactcgag
     1141 ccagtggccg tcggcgtcga tgaccggatg cgagagacgg gcgtgaaggg tgcgggcgtc
     1201 gaggtggccg tggtgactca tgggtgggga gggcctccag agagtcgggt gaagttcgct
     1261 acggggcggg cggggccacc gagacgccct tcgcgccggt cctctcgatg gcgcgggcca
     1321 cgagaccgga ccgcttggcg tcctcgacga cctggcgcag gtagcgcagg ccggcgtcct
     1381 tgcccttcgg caccgcgatg gattgctgca cgcccatgaa ccgcccctcg accaggcggg
     1441 cgcccgggag cttcggcgcg aggccgagca ggccctgcgt caacccggcg agggcgtcca
     1501 ccttgccgga tgcgagcagc tcgaacgccg cgtcggcgcc tgccgtcgag acgagctggg
     1561 cgcgcttgag gttgcggctc aggtagagct cgtagttggc gcgggccggc gcggcgaccc
     1621 gcacgccctc gcgatccacc tccgcggcgg tgcgcagggg cgagccgccc ggcacgagat
     1681 aggtagcctc gatctcgaga tacgcggcgg tgaagctcat gatcttctcg cgcgccggat
     1741 cggacccgag gaacgcgatg tcccacaccc cgctcggcgc cgcgtcgccc atgaccgcca
     1801 cgctgtcgta ggggacgatc tcgaccggca ccccgagccc cttgccgacc tcctgggtca
     1861 aatcgacggc cacgccccgg gactcgccgg tggccttgtc cttcgtggcg aggatgaagt
     1921 tgccgtagtt gatggcggcg cgcagcttgc cggtcggggc gaggtcggcg cggacggcgg
     1981 gactcatgtc ggccatggcg ccagatgcta gcagaatcgg gcccgcaaat cctatggccc
     2041 ctgcaagcgt gcgcccaagc gtgcgcccaa gcgtgccccc aagcgtgccc ccaagcttgc
     2101 ccatggtcgg cgatcacgag gctaggccgc gcctatgaac gtgtccaggc gaggacttgc
     2161 gaattcttgc ctcccgtcgc cgggccggca tccgtgaccg ggttcccgac atcccaaccc
     2221 ccaggaggta cgtgacgatg cgtaagctcg aagacaaggt ggcgatcgtg accggcgcgt
     2281 ccaaggggat cggggcctcg atcgccaagc acctcgcggc agagggggca gcggtggtcg
     2341 tcaactacgc gtccagccgg gagggcgcgg accgggtggt cgccgagatc gcgcgggcgg
     2401 gaggccgggc cctcgcgatc caggccgacc tcgccaagcc cgccgatgtc gatcggctct
     2461 tcgcggagac cgcgaaggcc ttcggccggc tcgacgtcct ggtgaacaac gcggggatct
     2521 acgagttccg acccctcgag gacatcaccc cggagcactt ccacaagtac ttcgacgtca
     2581 acgtgctcgg ggtgctgctg gcgtcgcggg cggcggtcaa gcacttcggc cccgcgggcg
     2641 gcagcatcat caacatcagc tcgggcgcca cccggctgac cccggccaac tccgcggtgt
     2701 acagcgcgac caaggcggcc gtggaagcca tcaccggggt cctcgccaag gagctggggc
     2761 cgcggcgcat ccgcgtgaac gcggtccttc cgggggtggt gctgaccgaa ggggttcgcg
     2821 ccggcggctt cgacgaaggc gacttccgca aggagatgga ggcgcggact ccgctcgggc
     2881 gcatcggcca gcccgaggac atcgctcccg cggtggtctt cctggccgcg gaggactccg
     2941 cctggatcac cggcgagtcg ctgctcgtcg cgggcgggct gcgctagaag ggcgctaccc
     3001 gggccggcgg gccggagaaa tcgcgcgggg aagccgggcg ggtggcttac ggtagtgagc
     3061 ggtgag
//
LOCUS       DATMCV010000003         5606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_133434, whole genome shotgun sequence.
ACCESSION   DATMCV010000003 DATMCV010000000
VERSION     DATMCV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133434"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..538)
                     /locus_tag="VNU03_00060"
     CDS             complement(149..538)
                     /locus_tag="VNU03_00060"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006517057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP72864.1"
                     /translation="MDLEFLNFPAAPPPPPTAAYSHAVRAGEYLFVTGQLGVDPKTNT
                     LVPGGTVAQTHQVMRNLQAVLAGAGTTLARAVMVRIYLTNFADYGAMNAAYASYFAAG
                     KLPARTTVGVTALALAAAVEIDLIVRM"
     gene            complement(550..1473)
                     /locus_tag="VNU03_00065"
     CDS             complement(550..1473)
                     /locus_tag="VNU03_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP72865.1"
                     /translation="MLALLRQNWLSAVGGIVALLIVLIALAAPLIAPRDPLKADFRHM
                     NKAPSTEAFFGTDQVGRDALSRVIYGARTSLFVAFSAVLLGTTLGSLWGVACGYLGGR
                     FDLLSQRVMEIFQSFPDLILAMAISMAIGTGLPAVIVAIAITRVPFGGRVIRSVALSV
                     SAMPFVEAGRAAGASQFRVMALHVLPQCVAPYLVLATTHLGVAIVIEAALGFLGVGVP
                     PPTPTWGNMLADAITGLVPQWWLVLFPGLAITVTVLAFNLLGNGIRDSLDPKLAPTVL
                     RLVTGVGRGARSAGTTTATPAPSAQGQPGAA"
     gene            complement(1484..2440)
                     /locus_tag="VNU03_00070"
     CDS             complement(1484..2440)
                     /locus_tag="VNU03_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP72866.1"
                     /translation="MQKYLLRRGLFALSTVIGVSLIVFVVLRILPGDPLVALLGVEGH
                     SKMSPADRVIIMKDLGLSDSLPVQYVRWLRDIASGQLGKSFFRGDTVRDLILHRGPLS
                     AEIAILALIISWLVGLPVGILSAVRPNGWADAVTRFFSILFIAIPGFWLSMLLVLALL
                     FWFGYKSPIMVVHLWENPWQNLQIIIGPAIVLGLAQGAYIARMSRSCLLEVIREDYVR
                     TARAKGAVERAVVLRHALPNALLPVVTISGVLLGFVLGGSVAVEQAFGVPGLGRALVT
                     AIIERDIIVVQNLVLLYALIFVGVNALVDLSYAWLDPRIRYQ"
     gene            complement(2456..4117)
                     /locus_tag="VNU03_00075"
     CDS             complement(2456..4117)
                     /locus_tag="VNU03_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP72867.1"
                     /translation="MADPVPHSRRSFLKTLGVAGAAAGVAPAFLRSRADAQEFQLKAA
                     DPAAKPGGTLRFGILNAPAHFDVHQSGTIANLGPQAPMYDLLIRRDPRDGNTIVPDLA
                     HKWEIAPDGKKYTFYLRKGVKFHDGAEMTSADMKATYDRIVRPPKGVVIPRSSLFTAV
                     GDILTPDPYTIEFRLTEPRPRAFMLGAFASGWNIVVRKKTLDDNQGNLRQVMNFPGTG
                     PFRHVSRKDKEVWIQEKNPNYWNKGLPYVDKLEIYNLPPFSPELGSSFLSGKVDYARI
                     MDPVSWRKAKEMPGVTATAMNQSVIQAYFFNMKRKPFEDPRVRRALHLATDRHVLVDV
                     VKDTAPMQVGGFVYPFHEWSAQKPEMEKRLGYQKDPKAAIQEAKKLMAAAGHPNGLKN
                     LDFVVRDIASFKLWAVAIQAMLKENLNVETNLRVVQTSQWFEEAGSGNFDLAISAIVS
                     SLMDPSDYFTAWYGKGGPQNYSGWTNEQFHSLAHNLEREVDENKRKAMIPKAEEILEN
                     DPPLVPVAWEQIYDAFYNRVRGQNATKFFGIYDVTRWDTVWMAQS"
     gene            4286..5362
                     /locus_tag="VNU03_00080"
     CDS             4286..5362
                     /locus_tag="VNU03_00080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP72868.1"
                     /translation="MEFGILFTSHPNHATEPYPHRAVHARVTAEIQAADRLGYDTAWV
                     AEHHFSNQYGIMPDVFTYMGYLAAQTTRIRLGTAVVTLPLYDPIRVVENMAFVDILSG
                     GRVVLGLGSGYRPYEFEGFGRDFEHRRDVQEEAIDLILELLHKRRVRHQGPHFKSVIE
                     GEYEIFPVSEQQPHPPLFMAGATDRSLTYAARHGFGLMLSTLPSADTLARQITLYRSH
                     LKEAPAPLDQNPAAGKVDIARWVYVADTDASAKRDTEQGIVRHISHFMGAGTAGYLGN
                     VSEKTRGESLSYDDLAATTLLHGSPATVIAKLRELRHKTGLTSLLLHYPPYYGHEKAM
                     QSLRLFAEKVMPEFRPPVERTAVA"
     gene            complement(5479..>5606)
                     /locus_tag="VNU03_00085"
     CDS             complement(5479..>5606)
                     /locus_tag="VNU03_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWP72869.1"
                     /translation="LGERLAPRHFAGMGLIAGGLAVIDGRLLPELLARKAGRQAG"
BASE COUNT          950 a         1897 c         1925 g          834 t
ORIGIN      
        1 gttgtcaaca tccatgaact tgaagcgttc gcggaagatc aattcgaaga cttttaacga
       61 agcgactgct gcctcgaccg ccatctttaa ggcttacatt acgacaatgt cgtaatgtaa
      121 gacctgaccc caaactacat acgccaaact acatacggac gatgaggtcg atctcgacgg
      181 cggcggcgag ggcgagggcg gtgacgccga cggtggtgcg ggcgggaagc ttgccggccg
      241 cgaagtagga ggcgtaggcc gcgttcatcg cgccatagtc ggcgaaattg gtgaggtaga
      301 tgcgcaccat caccgcgcgg gccagcgtcg tgcccgcgcc ggccaggacc gcctgcagat
      361 tccgcatcac ctggtgcgtc tgcgccacgg tgccgcccgg caccagcgtg ttggtcttcg
      421 ggtcgacgcc gagctgcccg gtcacgaaga ggtactcgcc cgcccggacc gcgtggctgt
      481 aggccgcggt gggcggcggc gggggcgcgg ccgggaagtt gaggaactcc aggtccaccg
      541 ggtgcgccct caggcggccc cgggctggcc ctgggcggac ggggcgggcg tggcggtggt
      601 cgtgccggcg ctgcgcgccc cgcgtcccac tccggtcacg agccgcagca cggtgggcgc
      661 gagcttgggg tcgaggctgt cgcggatgcc gttgcccagc aggttgaagg cgagcacggt
      721 caccgtgatg gcgaggccgg gaaagagcac gagccaccac tgcggcacga gcccggtgat
      781 cgcgtcggcc agcatgttgc cccaggtggg ggtgggcggc ggcacgccga cgccgaggaa
      841 gccgagcgcg gcctcgatca cgatagccac gccgaggtgc gtggtggcca gcaccaggta
      901 gggcgccacg cactggggca gcacgtgcag ggccatcacc cggaattgcg aggcgcccgc
      961 ggcgcgcccg gcctccacga acggcatcgc gctcacgctg agcgccaccg agcggatcac
     1021 ccgcccgccg aagggcacgc gcgtgatcgc gatggccacg atcaccgcgg gcagcccggt
     1081 gccgatggcc atcgagatcg ccatcgcgag gatcaggtcc gggaaggact ggaagatctc
     1141 catcacccgc tggctcagca ggtcgaaccg cccgcccagg tacccgcacg ccacccccca
     1201 gagcgagccg agggtggtgc cgagcagcac cgcggagaag gccacgaaga gcgaggtgcg
     1261 cgcgccgtag atcacgcggc tcagcgcgtc gcgccccacc tggtcggtgc cgaagaaggc
     1321 ctcggtgctc ggggccttgt tcatgtgccg gaagtcggcc ttgagcggat cgcggggcgc
     1381 gatgagcggc gcggccagcg cgatcaagac gatgaggagc gcgacgatgc cgccgaccgc
     1441 cgacagccag ttctgacgca ggagcgcgag catcgaaagc gggttactgg tagcggatgc
     1501 gcggatcgag ccacgcgtag gagagatcca ccagcgcgtt caccccgacg aagatgagcg
     1561 cgtagagcag caccaggttc tgcaccacga tgatgtcgcg ctcgatgatg gcggtgacga
     1621 gggcgcgccc gagcccgggc accccgaagg cctgctccac cgccaccgag ccgcccagca
     1681 cgaagccgag cagcaccccg gagatggtga ccaccggcag gagcgcgttg ggcagggcgt
     1741 ggcgcagcac caccgcgcgc tccaccgcgc ccttggcgcg cgcggtgcgc acgtagtcct
     1801 cgcggatgac ctccaggaga cacgagcgtg acatccgcgc gatgtaggcc ccctgggcga
     1861 ggccgaggac gatcgcgggc ccgatgatga tctgaagatt ttgccagggg ttctcccaga
     1921 ggtgcaccac catgatcggc gacttgtagc cgaaccagaa cagcagcgcc agcaccagca
     1981 gcatgctgag ccagaagccg gggatcgcga tgaagaggat ggagaagaag cgggtgaccg
     2041 cgtcggccca cccgttgggg cgcaccgcgc tcaggatgcc caccggcagg cccaccagcc
     2101 acgagatgat cagggccagg atcgcgatct cggccgagag cggcccccgg tgcaggatca
     2161 ggtcgcggac ggtgtcgccg cggaagaacg acttgccgag ctgcccgctg gcgatgtcac
     2221 gcagccagcg gacgtactgc accgggagcg agtccgacag ccccaggtcc ttcatgatga
     2281 tcacgcgatc cgccggtgac atcttggagt gaccttccac tccaagaagg gccaccagcg
     2341 gatcgccggg caggatgcgc agcaccacga acacgatcag ggagaccccg atcacggtgc
     2401 tcagtgcgaa cagcccccgc cgaagcagat acttctgcac gtgcgcgaat cggccctagg
     2461 actgggccat ccagaccgtg tcccagcgcg tgacgtcgta gatgccgaag aacttcgtcg
     2521 cgttctggcc gcggacccgg ttgtagaacg cgtcgtagat ctgctcccag gccaccggca
     2581 ccagcggcgg atcgttctcg aggatctcct ccgccttggg aatcatcgcc ttgcgcttgt
     2641 tctcgtccac ctcgcgctcg aggttgtgcg cgagggagtg gaactgctcg ttggtccagc
     2701 ccgagtagtt ctgcggaccg cccttgccgt accaggcggt gaagtagtcg gaggggtcca
     2761 tgagggagga gacgatcgcg ctgatcgcga gatcgaagtt gccagagccc gcctcctcga
     2821 accactgcga ggtctgcacc acgcgcagat tcgtctcgac gttgaggttc tccttcaaca
     2881 tcgcctggat cgccaccgcc cacagcttga agctggcgat gtcgcgcacc acgaagtcga
     2941 ggttcttgag cccgttcggg tgacccgcgg cggccatcag cttcttggcc tcctggatcg
     3001 ccgccttcgg gtccttctga tagccgaggc gcttctccat ctccggcttc tgggcggacc
     3061 actcgtggaa ggggtagacg aagccgccga cctgcatcgg cgcggtgtcc ttcaccacgt
     3121 cgaccagcac gtgccggtcg gtggccaggt gcagcgcccg gcgcacgcgc ggatcctcga
     3181 acggcttgcg cttcatgttg aagaagtacg cctggatgac cgactggttc atcgcggtcg
     3241 cggtgacccc cggcatctcc ttggccttgc gccaggagac cggatccatg atccgcgcgt
     3301 agtccacctt gccggagagg aacgacgacc ccagctcggg cgagaacggc ggcaggttgt
     3361 agatctcgag cttgtcgacg tatggcagcc ccttgttcca gtagttgggg ttcttctcct
     3421 ggatccagac ttccttgtcc ttccgcgaga cgtggcggaa gggtccggtg cccgggaagt
     3481 tcatcacctg ccgcaggttg ccctggttgt cgtccagcgt cttcttgcgc acgacgatgt
     3541 tccagccgct cgcgaaggcg cccagcatga aggcgcgggg ccggggctcg gtcaggcgga
     3601 actcgatggt gtacgggtcc ggcgtcagga tgtcgcccac cgcggtgaag agcgaggagc
     3661 gcgggatgac cacgcccttg ggcggccgca cgatgcggtc gtaggtggcc ttcatgtccg
     3721 ccgaggtcat ctcggcgccg tcgtggaact tcacgccctt gcggagatag aaggtgtact
     3781 tcttgccgtc cggcgcgatc tcccacttgt gggccaggtc cgggacgatg gtgttgccgt
     3841 cgcgcggatc gcgacggatc agcaggtcgt acatcggggc ctgcggcccg aggttggcga
     3901 tcgtgcccga ctggtggacg tcgaagtggg ccggcgcgtt caggatgccg aagcgcaagg
     3961 tgccgcccgg cttggcggcc ggatcggcgg ccttgagctg gaactcctgc gcgtccgcgc
     4021 gagagcggag gaaggcgggg gcgacgcccg cggcggcgcc cgcgacgccg agtgtcttca
     4081 gaaacgagcg acggctatga ggtaccggat cggccatggc gttcccctcc tttggggtct
     4141 ccacactgcg ggtcgattgc atccggaccg aggcgatggg tccacggaag ataacacggg
     4201 ccgcccggtc cctggcaaga cgctttctgc tagactcgac gccaactcac gctcgtcccg
     4261 ccaactctcc cggaggacaa tcggcatgga attcggcatc ctgttcacgt cgcaccccaa
     4321 tcacgctacc gagccctacc cgcaccgcgc cgtccacgcc cgcgtcaccg ccgagatcca
     4381 ggccgccgac cgcctcggct acgacaccgc ctgggtggcc gagcaccatt tctcgaacca
     4441 gtacggcatc atgccggacg tcttcaccta catgggctac ctggctgccc aaaccacgcg
     4501 catccgcctc ggcaccgcgg tggtgacgct ccccctctac gatccgatcc gcgtcgtcga
     4561 gaacatggcc ttcgtggaca tcctctcggg cggccgcgtg gtgctggggc tgggctcggg
     4621 ctaccggccc tacgagttcg agggcttcgg gcgggacttc gagcatcgcc gcgacgtcca
     4681 ggaggaggcc atcgacctga tcctggagct cctgcacaag cgccgcgtgc ggcaccaggg
     4741 accccacttc aagtcggtca tcgagggcga gtacgagatc ttcccggtca gcgagcagca
     4801 gccccacccg ccgctcttca tggccggggc caccgaccgc tcgctcactt acgcggcgcg
     4861 gcacggcttc gggctcatgc tctcgaccct gccctcggcc gacaccctcg cccggcagat
     4921 cacgctctac cggagccacc tgaaggaggc gcccgccccg ctcgaccaga atccggccgc
     4981 cggcaaggtg gacatcgcgc gctgggtcta cgtggccgac accgacgcgt cggccaagcg
     5041 cgacaccgag cagggcatcg tgcggcacat cagccacttc atgggggcgg gcaccgcggg
     5101 ctacctgggc aacgtctcgg agaagacccg cggggagagc ctgagctacg acgacctggc
     5161 cgcgaccaca ctgctgcacg gctcgcccgc caccgtcatc gccaagctcc gcgagctgcg
     5221 ccacaagacc gggctcacgt ccctgctgct ccactacccg ccgtactacg gccacgagaa
     5281 ggcgatgcag agcctgcggc tgttcgcgga gaaggtgatg ccggagttcc ggccgccggt
     5341 ggagcggacg gcggtggcct gagcggcagg agccggcgcg gcgagacggg tcacgggtac
     5401 cgacagcaat cctcagcagg cgtggagtcg gacgctgcgc gcggccgccc cggctctctg
     5461 cgtggatcac cgcgaccgct atcctgcctg gcgcccggcc ttccttgcga gcagctcggg
     5521 aaggaggcgc ccgtcgatga cggcgaggcc cccggcgatg agccccatgc ccgcgaagtg
     5581 tcgcggcgcg aggcgctcgc cgagca
//
LOCUS       DATMCV010000004         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_598639, whole genome shotgun sequence.
ACCESSION   DATMCV010000004 DATMCV010000000
VERSION     DATMCV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598639"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VNU03_00090"
     CDS             complement(<1..624)
                     /locus_tag="VNU03_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP72870.1"
                     /translation="MARRACWLRVLTAFAMVAAFMIAPGPADAQKKGGVLRVGNLGEP
                     PSLDAHWTTASITETLTNHIYEGLFSLDRDNKPIPMLAESYTVSKDGLTYTFKLRQGV
                     KFHNGKEMTSDDVVASLTRWGKQSIYGKALFAQVAELRAVDPATVQMQLKEKSAIVLI
                     SLAVPNNFGAIYPKEIAEKFAPEVKVTEYVGTGPYKLAEWKPDQYIRM"
     gene            690..1193
                     /locus_tag="VNU03_00095"
     CDS             690..1193
                     /locus_tag="VNU03_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP72871.1"
                     /translation="MSTAPCPARLRSYGRRRHPAHRAPVSDLDRSVAFYRDLLGLELI
                     AQWDSSQPYLRAIVAYPDAELRIALLRLPAAGDGGPGHHIELLEYRRPRGARGDTNTF
                     NPGNGHVAFMVTDIDATYADLKARGVRFKSAPVPITHGRNAGAKGVYFFDPDDITLEL
                     IQPAPRA"
     gene            1190..1381
                     /locus_tag="VNU03_00100"
     CDS             1190..1381
                     /locus_tag="VNU03_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP72872.1"
                     /translation="MTSLDLFRLDGQAAVVVGGAGGLGAAMARGLAEAGAAVAVADTS
                     AGAAQWPTTSRRRAAAPSR"
     gene            1336..1953
                     /locus_tag="VNU03_00105"
     CDS             1336..1953
                     /locus_tag="VNU03_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP72873.1"
                     /translation="MADDIAKAGGRALAVTVDVADRASVERMAAEIEERLGPIEMLVN
                     SAGITHRSPAADFPEPEWHRVIAVNLTGVFNGCQVVGRRMLARRRGRIVNIASIAGQI
                     GLTGTVAYAASKGGVVMLTRALAVEWAPHNVRVNAIAPSWFESNIGDLIHREPGYAER
                     AMRRVPIGRMGTPDELIGAALYLASDASSMVTGHVLAVDGGTLAS"
BASE COUNT          291 a          724 c          742 g          332 t
ORIGIN      
        1 catgcggatg tactgatcgg gcttccactc cgcgagcttg taggggccgg tgcccacgta
       61 ctcggtgacc ttcacctcgg gggcgaactt ctccgcgatc tccttcgggt agatcgcgcc
      121 gaagttgttg ggcaccgcga gtgagatgag cacgatggcc gacttctcct tgagctgcat
      181 ctgcacggtg gccggatcga ccgcgcgcag ctccgcgacc tgggcgaaga gggccttgcc
      241 gtagatcgac tgcttgcccc agcgggtgag cgaggcgacc acgtcgtcgg aggtcatctc
      301 cttgccgttg tggaacttca cgccctgccg gagcttgaag gtgtaggtga ggccgtcctt
      361 gctgacggtg tagctctccg cgagcatcgg gatcggcttg ttgtcgcggt cgaggctgaa
      421 gagcccctcg tagatgtggt tggtgagggt ctcggtgatg ctggcggtgg tccagtgcgc
      481 gtcgagcgag ggcggctcgc ccaggttgcc gacccgcagc acgccaccct tcttctgcgc
      541 gtcggcgggg cccggtgcga tcatgaatgc cgcgaccatc gcgaacgcgg tcaggacccg
      601 tagccagcat gctcgacgcg ccatacctgc acctccggaa gtgagcgggt gggtgttggg
      661 tgcccagact ctgcgatgag tggtgcggct tgtcaaccgc cccgtgcccg gctagattgc
      721 gctcctatgg gcgtcgccgc catcctgcac accgggctcc cgtcagcgat ctggaccgct
      781 cggtcgcgtt ctaccgcgac ctcctgggcc tcgagctgat cgcccagtgg gacagcagcc
      841 agccatactt gcgggccatc gtcgcgtatc ccgacgccga gctgcgcatc gcgctgctcc
      901 ggctgcccgc ggccggcgac ggcgggccgg gccaccacat cgagctgctc gagtaccggc
      961 gcccgcgcgg ggcgcgcggc gacacgaaca ccttcaaccc cggcaacggc cacgtggcgt
     1021 tcatggtgac ggacatcgac gcgacctacg ccgacctgaa ggcccggggc gtccgcttca
     1081 agtcggcgcc ggtgccgatc actcacgggc ggaacgcggg ggccaagggg gtgtacttct
     1141 tcgacccgga cgacatcacc ctcgagctga tccagccggc gccgcgggca tgaccagcct
     1201 cgacctcttc cggctggacg gccaggccgc ggtggtggtg ggcggcgcgg gcgggctcgg
     1261 ggccgcgatg gcgcgcgggc tcgcggaggc cggcgccgcg gtcgcggtgg cggacaccag
     1321 cgcgggcgcg gcccagtggc cgacgacatc gcgaaggcgg gcggccgcgc cctcgcggtg
     1381 acggtcgacg tcgccgatcg ggcgagcgtc gagcggatgg ccgcggagat cgaggagcgc
     1441 ctcggcccga tcgagatgct cgtcaacagc gccggcatca cccaccgcag tcccgccgcc
     1501 gactttcccg agcccgagtg gcaccgcgtg atcgcggtga acctcaccgg cgtcttcaac
     1561 ggctgccagg tggtggggcg gcgcatgctg gcccgccggc gcggccgcat cgtcaacatc
     1621 gcgtccatcg cgggccagat cgggctcacc ggcacggtgg cctacgcggc cagcaagggc
     1681 ggggtggtca tgctgacgcg ggccctcgcg gtcgaatggg cgccgcacaa cgtgcgggtc
     1741 aacgccatcg cgccttcgtg gttcgagtcc aacatcggcg acctgatcca ccgcgagccc
     1801 ggctacgcgg agcgcgccat gcgccgcgtg ccgatcgggc gcatggggac tccggacgag
     1861 ctgatcggcg ccgcgctcta cctcgcctcc gacgcctcca gcatggtcac cggccacgtc
     1921 ctcgccgtcg acggcggcac cctggcctcc tagctcgggg tcaggtctta cattccgaca
     1981 ttttcccgcc gcggccctcg ccagcgttgg cggaaatgtc ggaatgtaag acctgacccc
     2041 acctggcgcc cgtctcagcg ctggagggat tcgtggatcg cgagggtga
//
LOCUS       DATMCV010000005         6673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_199750, whole genome shotgun sequence.
ACCESSION   DATMCV010000005 DATMCV010000000
VERSION     DATMCV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199750"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VNU03_00110"
     CDS             <1..1088
                     /locus_tag="VNU03_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP72874.1"
                     /translation="FGHAVLLGLGGYVSAHVITASKAGALPLPLEATPLVGGLGGLFF
                     AIVLGYFATKQRATAFAMITLGIGELITACALMFQTFFGGEGGVSTNRMTGHSLFGLA
                     YGASIQVYYLIVAWTAISIALMLLLTRTPLGRMANACRDNFERAQFVGYDPRRVRFFQ
                     FALSGFFAGIAGALYTLTYEIVTFDTVAASMSANALLMTYIGGVGVFAGPVIGAVLIV
                     LLQSWVSLLSNSWLVYAGVLFILMVTFAPGGIGGLVMMHQPIWRARRLGRLAGPYLRL
                     LVPGLLVVVGFVLVVELCSFLTIGAAQGKSFSLSRVTIEPRTPLPWAVAAVLLVGGGL
                     WLRHAAGGFARAWHAVVDEIKAAGMRA"
     gene            1085..1849
                     /locus_tag="VNU03_00115"
     CDS             1085..1849
                     /locus_tag="VNU03_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP72875.1"
                     /translation="MTVPALRLIDVEKSFGPTAVIMGVSLDVTTGSRHAIIGPNGAGK
                     TTLFNLVSGRFPLSAGRIELHGRRIDGLEPHEITRRGLSRSFQVTSIFSRMSVFENIR
                     CGLLWSRGYRYSFWHLLGRERALNEAAERLLADVNLEARRDLPAGLLSYAEQRALEIG
                     ITIAGGADTVLLDEPTAGMSRSEADHAVALIRRITAGKTLVMVEHDMSVVFDLADVVT
                     VLVYGEVIASGPPAEIRANRAVQDAYLGAPVETGIA"
     gene            1849..2544
                     /locus_tag="VNU03_00120"
     CDS             1849..2544
                     /locus_tag="VNU03_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP72876.1"
                     /translation="MLSVRDLHAYYGKSHILQGVNLEVGGGEIVSLLGRNGVGRSTTC
                     KAIMGLVEPVGEVIWKGQSLAGKRADVIAHAGIGYVPEDRQVFPTLTVRQNLELGLKQ
                     AGRFGRWSFDDMFDLFPHLRERQHNIAGVLSGGEQQMLTMGRTLMGDPELVIIDEPTE
                     GLAPMLVTQVGRFLDEIARRGVSILLVEQKLTIALKISHRVYVMGHGRIVFEGTPAAL
                     AANAAVRKENLEV"
     gene            2541..2948
                     /locus_tag="VNU03_00125"
     CDS             2541..2948
                     /locus_tag="VNU03_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWP72877.1"
                     /translation="MKTPRKGFRQMVDEAKSRIKTISLEEAKQRLGSDRVVFVDLRDV
                     RELEREGMIPGAFHCPRGMLEFWIDPDSPYHKDVFDADKEFVFYCNGAWRSALAADVA
                     QQMGLESVVEMDGGFSEWKSKGFPVAERPSKKA"
     gene            complement(2945..3289)
                     /locus_tag="VNU03_00130"
     CDS             complement(2945..3289)
                     /locus_tag="VNU03_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72878.1"
                     /translation="MTRWLVIVGLVLGATAAIGVDHAAASMASIVTVAPVVEDEGQAG
                     VEAAAASALRRAVRGAQAMGLDEMMLKSIRLVPGTGVVVEIIASESNEPDADTDGDVE
                     RSKLEIAPRTRL"
     gene            3498..3989
                     /locus_tag="VNU03_00135"
     CDS             3498..3989
                     /locus_tag="VNU03_00135"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HWP72879.1"
                     /translation="MASQNENDPPAEEQQALPMILPGNAQGPVVEQPAKLIRIASMIR
                     ELLQDVRQSQPDEAGRKRLGEVYQRALAALKEGVSKELQDELETLVLPLENTPSESEV
                     RLAQAQLLGWLEGLFQGIQAALWTQHMQARAEIDEMRRRRGLPPGPGGPEMGPPRDPG
                     QYL"
     gene            complement(4020..4193)
                     /locus_tag="VNU03_00140"
     CDS             complement(4020..4193)
                     /locus_tag="VNU03_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72880.1"
                     /translation="MPSYTMTCTHCGYEVIFRTEPDAKTEGVRHLRQQPTHGVKVTPS
                     EDALIREAEKITR"
     gene            4280..5521
                     /locus_tag="VNU03_00145"
     CDS             4280..5521
                     /locus_tag="VNU03_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP72881.1"
                     /translation="MTSCRITNVRILDGTGREPFAGSVRVEGNRIAEVSAGAGPAGPP
                     ADRVIDGRGATLMPGLIEPHAHLSFADVVSYELTRLPPEEHLLATIRNARTMLDSGYT
                     SALSAAAAKPRLDVVLKREIDAGRLPGPRTLANGPEITVTGGLGDSNLLHLPYHETPT
                     FCWVADGPDAIRRVCRLLAREGVDLLKLNLSGDLGTSAWSSELTPMSEAEVGAAMEVA
                     CARGLRVCAHCRSGDSVRMAVRHGIEIVYHANYCDEQALDALEAARDRVFVAPALGIT
                     YNLTQANGQWGITPDRAVGFERELAASVETIGRMRARGIRVLPGGDYGFVYTPHGTYA
                     RDLWLFVKLLGFSPMETLVAATRLGGEIMGQPGELGVVKAGALADLLLVDGDPLADIA
                     ILQDRSALRMIMKDGALHKAP"
     gene            5534..6226
                     /locus_tag="VNU03_00150"
     CDS             5534..6226
                     /locus_tag="VNU03_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWP72882.1"
                     /translation="MSRARLFRSIKALDADAMAELLAAQPELARATDDRRRNPLHFLC
                     SLPASPKTSDRSIRLARQLLAAGIAIDAPAFVEGAFQATPLWYAISRGRNGPLARFLL
                     KQGSSPECCLWAAGFRDDVETIDLLVQHGASIDPVTEDETPFLSAVKWSHFAAAERLL
                     HHGADVNFQNSKGVTALHLVLKKDSAPPHVAMLMRHGADPTIKSKDGKSPLDLVARRR
                     DKTYLTILSGRR"
     gene            6251..>6673
                     /locus_tag="VNU03_00155"
     CDS             6251..>6673
                     /locus_tag="VNU03_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020053063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWP72883.1"
                     /translation="MTPDERRYYATQSRVSDPEDRSALLAALPAEPADLVPAISGLVL
                     HTLFVRPLGITAPAGSEADVESRSVPRILDRILERDPAPLQVTRPPERRFIGICRDYA
                     LLACAALRQHRVPARLRVGFAAYFTPGYLEDHWVCEYHA"
BASE COUNT          944 a         2475 c         2247 g         1007 t
ORIGIN      
        1 ccttcggcca cgcggtgctc ctggggctcg gcggctacgt cagcgcgcac gtgatcaccg
       61 cgtccaaggc cggcgcgctc ccgctgccgc tcgaggcgac cccgctcgtg ggcgggctgg
      121 gcggtctctt cttcgcgatc gtcctcggct acttcgcgac caagcagcgc gccaccgcct
      181 tcgcgatgat cacgctcggc atcggggagc tgatcaccgc ctgcgccctc atgttccaga
      241 ccttcttcgg cggagagggc ggggtgagca cgaaccggat gaccggccac agcctcttcg
      301 ggctcgccta cggcgcgtcc atccaggtct actacctgat cgtggcctgg accgcgatct
      361 cgatcgccct gatgctcctg ctcacccgga cccctctcgg ccggatggcc aacgcctgcc
      421 gcgacaactt cgagcgcgcc cagttcgtgg gctacgaccc gcgtcgcgtc cgcttcttcc
      481 agttcgcgct ctccggcttc ttcgccggca tcgcgggcgc gctctacacc ctcacctacg
      541 agatcgtgac cttcgacacg gtcgccgcgt ccatgtccgc caacgccctg ctcatgacct
      601 acatcggcgg ggtgggggtc ttcgcggggc cggtgatcgg cgcggtgctg atcgtgctgc
      661 tccagagctg ggtgagcctg ctctccaact cctggctcgt ctacgcgggc gtgctgttca
      721 tcctcatggt caccttcgcg cccggcggca tcggcggcct cgtgatgatg caccagccga
      781 tctggcgggc ccgccggctg ggtcgcctcg ccgggcctta cttgcgattg ctggtccccg
      841 gcctgctggt ggtggtcggc ttcgtgctgg tggtggagct gtgctccttc ctcaccatcg
      901 gcgccgcgca gggcaagagc ttcagcctgt cgcgcgtcac catcgagccg cgcaccccgc
      961 tgccgtgggc ggtggccgcg gtgctgctgg tcggcggcgg tctctggctg cgccacgcgg
     1021 cgggcgggtt cgcgcgggcc tggcacgcgg tcgtggacga gatcaaggcg gcggggatgc
     1081 gcgcgtgacc gtccccgccc tgcgcctcat cgacgtggag aagagcttcg gccccaccgc
     1141 ggtcatcatg ggcgtgagcc tcgacgtgac gaccggctcg cgccacgcca tcatcggccc
     1201 gaacggggcg ggcaagacca cgctcttcaa tctcgtgagc ggccggttcc cgctctccgc
     1261 cggccgcatc gagctgcacg gccgtcgcat cgacggcctg gagccgcacg agatcacccg
     1321 gcgcggcctc tcgcgcagct tccaggtcac gtcgatcttc tcccgcatga gcgtgttcga
     1381 gaacatccgc tgcgggctcc tctggtctcg agggtatcgc tactccttct ggcacctcct
     1441 gggccgcgag cgggcgctga acgaggcggc ggaacggctc ctcgccgacg tgaacctcga
     1501 ggcgcggcgc gatcttccgg ccggcctcct ctcctacgcc gagcagcgcg ccctcgagat
     1561 cggcatcacc atcgcgggcg gcgccgacac cgtgctcctc gacgagccga ccgcgggcat
     1621 gagccggagc gaggccgacc acgcggtggc cctgatccgg cgcatcaccg cgggcaagac
     1681 cctggtgatg gtcgagcacg acatgagcgt ggtcttcgac ctggccgacg tggtcacggt
     1741 gctggtctac ggcgaggtca tcgcctccgg gccgccggcc gagatccgcg cgaaccgcgc
     1801 ggtgcaggac gcctacctcg gggcccccgt cgagaccggg atcgcctgat gctctcggtg
     1861 cgcgacctgc acgcctacta cggcaagagc catatcctcc agggcgtgaa cctggaggtg
     1921 ggcgggggcg agatcgtgag cctgctcggg cggaacgggg tcggccgctc gacgacgtgc
     1981 aaggcgatca tgggcctggt ggagccggtg ggcgaggtga tctggaaggg ccagagcctg
     2041 gccggcaagc gcgccgacgt catcgcccac gccggcatcg gctacgtgcc ggaggaccgc
     2101 caggtcttcc ccaccctgac cgtgcgccag aacctcgagc tggggctcaa gcaggccggg
     2161 cgcttcggcc gctggtcctt cgacgacatg ttcgacctct tcccccacct gcgcgagcgc
     2221 cagcacaaca tcgcgggcgt gctctcgggg ggcgagcagc agatgctgac catgggccgc
     2281 accctgatgg gcgatccgga gctcgtcatc atcgacgagc ccaccgaggg cctcgccccc
     2341 atgctggtga cccaggtcgg gcgcttcctc gacgagatcg cccgtcgggg cgtctcgatc
     2401 ctcctcgtgg agcagaagct caccatcgcg ctcaagatct cgcaccgggt gtacgtcatg
     2461 ggccacggcc gcatcgtgtt cgagggcacg ccggccgccc tggccgccaa cgccgcggtg
     2521 cggaaggaga acctcgaggt atgaagaccc cgcgcaaggg attccggcaa atggtggacg
     2581 aggcgaagtc ccgcatcaag accatcagcc tcgaggaggc caagcagcgc ctcgggagcg
     2641 atcgcgtggt cttcgtcgac ctgcgtgacg tccgcgagct ggagcgcgag gggatgatcc
     2701 ccggcgcgtt ccactgccct cgcggcatgc tcgagttctg gatcgatccg gacagcccct
     2761 accacaagga cgtgttcgac gccgacaagg agttcgtctt ctactgtaac ggggcctggc
     2821 gctccgccct cgccgccgac gtggcccagc agatgggcct cgagtccgtc gtcgagatgg
     2881 acgggggctt cagcgagtgg aagagcaagg gcttcccggt ggcggagcgg cccagcaaga
     2941 aggcctagag ccgcgtccgc ggggcgatct cgagcttcga ccgctcgacg tcgccgtcgg
     3001 tatcggcgtc gggctcgttc gactccgacg cgatgatctc cacgaccacg ccggtgcccg
     3061 gcaccagccg gatgctcttc agcatcatct cgtcgagccc catcgcctgc gcgccgcgga
     3121 ccgcgcggcg cagcgcgctc gccgccgccg cctcgacgcc ggcctgaccc tcgtcctcca
     3181 ccaccggggc caccgtcacg atggaggcca tgctcgcggc ggcgtgatcg acgccgattg
     3241 ccgcggtcgc tcccagcacc agtcccacga tcacgagcca gcgtgtcatt gtgatctccc
     3301 ttcccgatct cccgtgtcag ctcgatccgc tctgccatga gagtcaggca aggtccgtgc
     3361 ccgagcccac gacccgcggt tcccgcgctt cttccgaccg gcgcgtgaga cgcgcgcgat
     3421 ccgccggaac gtcttaccgc gttcccccga ccggatcggg gtcggggccg cttcgggagc
     3481 gtggtaggat tcgagccatg gcgagccaga acgagaacga tccgcccgcg gaagagcagc
     3541 aagccctgcc gatgatcctc cccggcaacg cgcagggccc cgtcgtcgag cagccggcca
     3601 agctgatccg gatcgcgtcg atgatccgcg agctgctcca ggacgtgcgc cagtcccagc
     3661 ccgacgaggc cgggcgcaag cggctcggcg aggtctacca gcgggcgctc gccgcgctca
     3721 aggaaggcgt gtcgaaggag ctgcaggacg agctcgagac gctggtcctg ccgctcgaga
     3781 acaccccgag tgaatccgag gtgaggctgg cccaggccca gctgctgggg tggctcgaag
     3841 gcctcttcca gggaatccag gccgctctct ggactcagca catgcaggcg cgcgccgaga
     3901 tcgacgagat gcgccgtcgc cgcggcctgc cgcctggtcc cggaggcccc gagatggggc
     3961 cgccccgcga cccgggccag tacctctagc cgcaggtccc ggggcggacc cggcggaagt
     4021 tacctggtaa ttttttcagc ctcgcggatc agcgcatcct cgctcggcgt caccttcacc
     4081 ccatgagtcg gctgctgccg aagatggcgt acgccttcgg ttttcgcgtc cggctcggtg
     4141 cgaaagatca cctcgtagcc gcagtgcgta caggtcatcg tgtagctcgg catggcgtcg
     4201 tcctcctcat gaaggcgcag taggaggcaa gagggatgcc atcgcgcacc tccgatcccg
     4261 gtgctagaat cccgccgaca tgacgagctg ccgcatcacc aacgtccgca tcctcgacgg
     4321 caccggccgc gagcccttcg ccggctccgt gcgcgtggag ggcaaccgca tcgcggaggt
     4381 gtcggccggc gcgggcccgg cggggccgcc cgccgaccgg gtcatcgacg ggcgcggcgc
     4441 gaccctgatg cccggcctga tcgagccgca cgctcacctg agcttcgcgg acgtcgtctc
     4501 ctacgagctg acccgcctgc cgccggagga gcatctcctc gcgaccatcc gcaacgcccg
     4561 caccatgctc gactcgggct acacgagcgc gctgtcggcg gccgcggcca agccgcggct
     4621 cgacgtggtg ctcaagcgcg agatcgacgc gggacggctg cccgggccgc gcaccctcgc
     4681 caacggcccc gagatcacgg tgaccggcgg gctcggcgac agcaacctcc tgcacctgcc
     4741 ctaccacgag acgccgacct tctgctgggt ggccgacggc cccgacgcca tccgccgcgt
     4801 ctgccggctc ctcgcgcgcg agggcgtcga cctgctcaag ctgaatctct cgggggacct
     4861 gggcacctcg gcctggtcgt cggagctcac gccgatgtcc gaggccgagg tcggcgcggc
     4921 catggaggtc gcctgcgccc ggggcctgcg cgtctgcgcc cactgccgca gcggcgactc
     4981 cgtgcgcatg gcggtgcggc acggcatcga gatcgtctac cacgccaact actgcgacga
     5041 gcaggcgctg gacgcgctcg aggccgcccg cgaccgcgtc ttcgtggctc ccgcgctcgg
     5101 catcacctac aacctgaccc aggccaacgg ccagtggggg atcacgccgg atcgcgcggt
     5161 gggcttcgag cgcgagctgg ccgcctcggt cgagaccatc ggccgcatgc gggcgcgcgg
     5221 catccgggtg ctgccgggcg gcgactacgg cttcgtctac accccgcacg ggacgtacgc
     5281 gcgcgatctc tggctcttcg tgaagctgct cggcttctcc ccgatggaga cgctcgtggc
     5341 ggccacccgc ctgggcgggg agatcatggg acagcccggc gagctcgggg tcgtgaaggc
     5401 gggcgccctc gccgatctcc tgctggtgga cggcgatccc ctcgccgaca tcgcgatcct
     5461 gcaggaccgc tccgcgctgc gcatgatcat gaaggacggg gcgctgcaca aggcaccctg
     5521 agggacggag gtcatgagcc gggcgcgcct cttccgctcg atcaaggccc tcgacgccga
     5581 cgcgatggcc gagctcctcg cggcccagcc ggagctggcg cgggccaccg acgatcgccg
     5641 ccgcaacccg ctccacttcc tctgcagcct gcccgcgagc ccgaagacgt ccgaccgatc
     5701 gatccgcctg gcgcgccagc tcctcgcggc cggcatcgcc atcgacgcgc ccgcgttcgt
     5761 cgagggcgcg ttccaggcga cgccgctctg gtacgcgatc tcccgcggcc ggaacggccc
     5821 gctggcccgg ttcctcctga agcagggctc cagcccggag tgctgcctct gggcggccgg
     5881 cttcagggac gacgtggaga ccatcgacct gctggtgcag cacggcgcga gcatcgatcc
     5941 ggtcaccgag gacgagactc cgttcctgag cgcggtcaag tggagccact ttgcggcggc
     6001 cgagcggctg ctccatcacg gggccgacgt gaacttccag aactcgaagg gcgtgaccgc
     6061 gctgcacctg gtgctcaaga aggacagtgc tccgccgcac gtcgccatgc tgatgcgcca
     6121 cggcgcggac ccgacgatca agagcaagga cggcaagagc ccgctcgacc tcgtggcccg
     6181 gcggagggac aagacctacc tgacgatcct ctccggtcgc cggtgagccc gacccggtga
     6241 ccccgccccc atgaccccgg acgagcgccg gtactacgcg acccagagcc gggtctccga
     6301 tccggaggat cggagcgcgc tcctcgccgc cctgcccgcc gagcccgcgg acctcgtccc
     6361 cgcgatctcc ggcctcgtcc tccacacgct cttcgtgcgg ccgctcggca tcaccgcgcc
     6421 ggcgggcagc gaggccgacg tcgagagccg gagcgtccca cgaatcctcg accgcatcct
     6481 ggagcgggac cccgcgccgc tgcaggtgac gcgcccgccc gagcgccgct tcatcgggat
     6541 ctgccgcgac tacgccctgc tcgcgtgcgc cgcgctccgc cagcaccgcg tcccggcacg
     6601 cctccgcgtc ggcttcgccg cctacttcac gccgggctat ctcgaggatc actgggtgtg
     6661 cgagtaccat gcc
//
LOCUS       DATMCV010000006         4036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_532282, whole genome shotgun sequence.
ACCESSION   DATMCV010000006 DATMCV010000000
VERSION     DATMCV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532282"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..651
                     /locus_tag="VNU03_00160"
     CDS             112..651
                     /locus_tag="VNU03_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72884.1"
                     /translation="MAQTPATSAQAQEAQLDPRLVLDEMKKNGVSHVVWLPDSETNFL
                     YQLMQADPSLTLVPVSREGLAFSIAAGLATGGQHPIILIQNTGLMESGDSLRGWAIGL
                     QIPVVMMVGYRGWTRHGVNTDSAATYTERFLNAFGITYYLVETSADAPRISIAFEEAR
                     RTQRPVAVLMADEYHGFNR"
     gene            663..1250
                     /locus_tag="VNU03_00165"
     CDS             663..1250
                     /locus_tag="VNU03_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWP72885.1"
                     /translation="MFQTADMLRIFNAHRGDAIVVPGRGGRHWAQISTRPTRDVPVGD
                     PAMGGHAGFALGLALARPADRVVLFDSEGDLLMSLGILPTVAELAPANFYHFMLDNEC
                     YATTGGQPVPNARNVAYDVIARGCGYPRAFAFDKLPDFEASLPSILAQPGPVFAALKV
                     EPEVENVPIGQRVRWQTRTRDRVLVDLRAELGVAR"
     gene            1256..3013
                     /locus_tag="VNU03_00170"
     CDS             1256..3013
                     /locus_tag="VNU03_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP72886.1"
                     /translation="MDAIDPVTLVVVQNGLQQVASEMDLTFERAAFSPVISEGFDRSD
                     GIYSRENGEVIAQGELGLPIFVGVMQFTTRAVIDHVKAAAEPLRPGDIFIVNDPYCGG
                     THLMDVKMVKPYFYRGELWAYLSNTGHWPDTGGSVPGGFSTRATEVQQEGLRLPPVKL
                     FREGVMQPDILSIILANIRVPEERIGDIKAQVAALTVGEKRLTALLDRYGLATVTACI
                     AELRRRSDLMMRAHIAKIPDGAYSGEAFVDSDGVDPDPLRISLRIRKDGTDLHFDFSE
                     SSGPCRGPLNSVIATTKAAVYLAVKHVFPDVPINAGCFEPLHIADPHGTFLYARYPRP
                     VSGCAAEVSSRIAESVFTTLAQAIPDDLFAAPAGTSGNLTLGGHDPLKERHYIMYVFS
                     GGGYGGSADGDGLTNGCSTIGISKTQPTEVLEQHYPILFERYALRERSGGAGKTRGGF
                     GVDYKIRIRRGEALLSFLMDHGRFGPPGLFGGRDGACNEVVVTRQGGDYVSPHLSKDE
                     DIRVVAGDAIAVGTPGGGGYGDPWQREPALVARDVARGYFTAADARRDYGVVLTAADP
                     PALDGRATAELRRGGRPKA"
     gene            complement(3037..3813)
                     /locus_tag="VNU03_00175"
     CDS             complement(3037..3813)
                     /locus_tag="VNU03_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP72887.1"
                     /translation="MDLQGQIAIVTGAGRGIGRATALELARLGADVVVAELDKASAER
                     TAGEVAALGRRALAVPTDVTRKADLTAMVERTRAQFGRLDILVNNAGIYRAAPTLQVT
                     EDHWDAVLTINARAVFFASQAALPTMIAQKGGTIVNLASMAGKIGSANNLPYNASKAA
                     VISITKSLALAHAADGIRVNCVCPGFVETDMWTSVSTELGALTGVLAEEFTRRRLASV
                     PLGRMERPEDVAGVIGFLASSRAGYMTGQAISVDGGLVMH"
     gene            complement(3828..3956)
                     /locus_tag="VNU03_00180"
     CDS             complement(3828..3956)
                     /locus_tag="VNU03_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72888.1"
                     /translation="MRRLYHLRMRATGQTGISDYDLGFTNFLYTFETSTTARQTPP"
BASE COUNT          585 a         1441 c         1424 g          586 t
ORIGIN      
        1 ttccaccacc gtgatcgagc cgggggaccg ggtgcgcgtg gacgcgctcg gcaacctgga
       61 gatcaccgtc cgcggcctgg aggccgcgac gtcaccggag acccgttgac catggcccag
      121 acccccgcga cctcagccca agctcaagaa gcccagctcg acccccgcct cgtgctcgac
      181 gagatgaaga agaacggggt cagccatgta gtgtggcttc ccgacagcga gacgaacttc
      241 ctctaccagc tcatgcaggc cgatccgtcg ctcacgctcg tgccggtgag ccgggagggc
      301 ctcgcgttct cgatcgcggc cgggctcgcg accgggggcc agcacccgat catcctgatc
      361 cagaacacgg gactcatgga gtcgggcgac tcgctgcggg gctgggcgat cgggctgcag
      421 atcccggtgg tgatgatggt cggctaccgc gggtggacgc gccacggcgt caacacggac
      481 agcgcggcaa cctacaccga gcgcttcctc aacgccttcg gcatcaccta ctacctggtc
      541 gagacctcgg ccgatgcccc gcgcatctcc atcgccttcg aggaggcgcg ccgcacccag
      601 cggccggtcg cggtgctgat ggccgacgag taccacgggt tcaaccgcta ggaggccgcc
      661 ccttgttcca gaccgccgac atgctccgga tcttcaacgc ccaccgtggc gatgccatcg
      721 tggtaccggg ccgagggggc cggcactggg cgcagatcag cacgcgcccg acccgcgacg
      781 tgccggtcgg cgaccccgcg atgggcgggc acgccggctt cgcgctgggc ctggccctcg
      841 cgcgcccagc cgaccgtgtc gtgctcttcg actccgaggg cgacctgctg atgagcctcg
      901 gcatcctgcc caccgtggcc gagctggccc cggccaactt ctaccacttc atgctcgaca
      961 acgagtgcta cgccaccacc ggcgggcagc cggtgcccaa cgcgagaaac gtggcctacg
     1021 acgtgatcgc ccgcggctgc ggctatccgc gcgcgttcgc gttcgacaag ctcccggact
     1081 tcgaggcgag cctgccgtcc atcctggcgc agccgggccc ggtcttcgcg gcgctcaagg
     1141 tggagcccga ggtggagaac gtcccgatcg ggcagcgggt ccgctggcag acccgcaccc
     1201 gcgaccgcgt gctggtagac ctgcgcgcgg agctgggcgt cgcgcggtag tcgggatgga
     1261 cgcgatcgat ccggtcaccc tggtcgtcgt gcagaacggc ctgcagcagg tggcgagcga
     1321 gatggacctc accttcgagc gggcggcctt ctcgccggtc atctccgagg gcttcgaccg
     1381 ctccgacggg atctactcgc gggagaacgg cgaggtcatc gcccagggcg agctggggct
     1441 gccgatcttc gtgggcgtca tgcagttcac gacgcgcgcg gtgatcgacc acgtgaaggc
     1501 ggccgcggag ccgctccggc ccggcgacat cttcatcgtc aacgatccct actgcggcgg
     1561 cacccatctg atggacgtca agatggtgaa gccctacttc taccgcgggg agctctgggc
     1621 gtatctctcg aacaccggcc actggcccga caccggcggc tcggtgccgg gcgggttctc
     1681 cacgcgcgcc accgaggtac agcaggaggg cctgcgcctg cccccggtga agctcttccg
     1741 ggaaggcgtg atgcagcccg acatcctcag catcatcctc gccaacatcc gggtgccgga
     1801 ggagcgcatc ggcgacatca aggcccaggt ggccgcgctc accgtgggcg agaagcggct
     1861 caccgcgctg ctcgaccgct acggcctagc caccgtgacc gcgtgcatcg ccgagctgcg
     1921 ccggcgatcg gatctgatga tgcgggcgca catcgccaag atcccggacg gcgcctactc
     1981 gggcgaggcc ttcgtggact cggacggggt ggacccggac ccgctgagga tcagcctccg
     2041 gatacggaaa gacggcacgg atctgcactt cgacttctcg gagtcgagcg ggccgtgccg
     2101 gggaccgctc aacagcgtga tcgccaccac gaaggccgcg gtgtacctgg ccgtcaagca
     2161 cgtcttcccg gacgtgccga tcaacgcggg ctgcttcgag ccgctccaca tcgcggatcc
     2221 gcacgggacc ttcctctacg cccgctaccc gcggccggtc tcgggctgcg cggccgaggt
     2281 gagctcgcgc atcgcggagt cggtgttcac cacgctggcg caggccatcc ccgacgacct
     2341 cttcgcggcg cccgcgggca ccagcggcaa cctcacgctg ggcggccacg acccgctgaa
     2401 ggagcggcac tacatcatgt acgtcttcag cggcggcggc tacggaggca gcgccgacgg
     2461 cgacggcctc accaacggct gctcgacgat cggcatctcc aagacccagc ccaccgaggt
     2521 gctcgagcag cactacccga tcctgttcga gcgctacgcc ctgcgggagc gctcgggcgg
     2581 ggcggggaag acgcgcggcg gcttcggggt ggactacaag atccggatcc gccgcggcga
     2641 ggcgctgctc tcgttcctga tggaccacgg ccgcttcggg ccgcccggcc tcttcggggg
     2701 ccgcgacgga gcgtgcaacg aggtggtggt cacccgccag ggcggcgact acgtctcccc
     2761 gcatctctcg aaggacgagg acatccgggt ggtggccggc gacgccatcg cggtgggcac
     2821 gccgggcggc gggggctacg gcgatccgtg gcagcgcgag cccgccctgg tggcgcggga
     2881 cgtggcgcgc gggtacttca ccgccgcgga cgcccggcgc gactacgggg tggtcctcac
     2941 cgcggccgat ccgcccgccc tcgacggccg cgccaccgcc gagctccgtc gcggaggtcg
     3001 tccgaaggcg taggggcgga ggcctcggcg gacggcctag tgcatgacga ggccgccgtc
     3061 gacgctgatg gcctggcccg tcatgtagcc ggcgcggctc gaggcgagga agccgatcac
     3121 gcccgccacg tcctccggcc gctccatccg gccgaggggc acgctggcca ggcgccggcg
     3181 ggtaaactcc tcggccagca ccccggtcag cgcgcccagc tcggtggaca cgctcgtcca
     3241 catgtcggtc tcgacgaagc ccgggcacac gcagttcacc cggatgccgt cggcggcgtg
     3301 ggccagggcc aggctcttgg tgatgctgat caccgcggcc ttgctcgcgt tgtagggcag
     3361 gttgttggcg ctgccgatct tgcccgccat cgaggccagg ttgacgatcg tgccgccctt
     3421 ctgcgcgatc atggtcggca gcgcggcctg gctcgcgaag aagaccgcgc gggcgttgat
     3481 ggtcagcacc gcgtcccagt gatcctccgt gacctggagc gtcggcgcgg cccggtagat
     3541 cccggcgttg ttcaccagga tgtcgagccg accgaactgc gcccgcgtcc gctcgaccat
     3601 cgcggtgagg tcggccttcc gggtcacgtc ggtcggcacc gcgagcgcgc gtcgcccgag
     3661 ggcggctacc tcgccggcgg tgcgctcggc cgaggccttg tcgagctcgg cgacgacgac
     3721 gtcggcgccc agccgggcca gctcgagggc ggtggcgcgg ccgatcccgc ggccggcgcc
     3781 ggtgacgatc gcgatctgtc cttgcaggtc cacgggggtc tcctccctca cggtggggtc
     3841 tgtcgggcgg tggtgctggt ctcgaaggtg tagaggaagt tggtgaagcc gaggtcgtag
     3901 tcggagatgc cggtctgccc ggtcgcccgc atgcgcaggt ggtagagccg gcgcatcgcg
     3961 ttctcgacct atcgctccac gccggtcagc ggcgcgcgca gcttcatcag gagggcccgc
     4021 agctcggggc ggagcg
//
LOCUS       DATMCV010000007         3961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_566, whole genome shotgun sequence.
ACCESSION   DATMCV010000007 DATMCV010000000
VERSION     DATMCV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VNU03_00185"
     CDS             complement(<1..620)
                     /locus_tag="VNU03_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWP72889.1"
                     /translation="MPSPRRRRRIVVVHVGRRMSRGVFRALEVEHDIFEAQGLRRGLA
                     LLRAQTGRVDLVVVMGLTQASGTPYVAGVTLLTDIFRYWPWIPVLVFAHPGPHTGLRA
                     DVLMSGVRAVLPHPAPGPTLKRAIRGILSRAPRRDPRHGATAMKRVRAFLDEHDDSRC
                     RLPDLARRAGLSRSYFSTRFHAVVGMSLREYRRTRRLARAHHLMMTT"
     gene            839..1747
                     /locus_tag="VNU03_00190"
     CDS             839..1747
                     /locus_tag="VNU03_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HWP72890.1"
                     /translation="MPTFAGYPASDDPSGAAIALLGVPDATPYTPGRSSHSAGAPAAM
                     RTALTGYATAPDHYDFDIDGLRPSGVVDCGDLVGDPSDPPGTRQRITAAIARFLEAGA
                     VPVVLGGDDSVVIPFFQAFAGRGPLTVVQVDAHLDWRDEVRGERFGWSSPMRRASEMP
                     WVERMVQVGLRGIGSARAGEVADARAWGSSIVTAAEVHRAGIGIAVDRVPAGARCLVT
                     IDCDGLDPAVMPGVSARAPGGLLYHHVLELLHGVAAKASIVGFDLVELMPDRDPSGIA
                     ALTAARLVCNGLAAIAVSQGDRAGGP"
     gene            1794..3338
                     /locus_tag="VNU03_00195"
     CDS             1794..3338
                     /locus_tag="VNU03_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP72891.1"
                     /translation="MIIPLTLADFLERAELVYGEREAIVDEPNPPGGGLGRITYRRFA
                     AMCRSMAASLDDLGVGEGERVAIVSPNAARFLVSLFGVSVFGRVLVPVNFRLNTEEIR
                     YIIEHSGSTVALIDPELEESLRPIPVKHRIVLGAASDAQLLDRRDARPRFGVSDENAT
                     VSINYTSGTTARPKGVQLTHRGCWLNAVTFGWHVGVSDRDVYLHTLPSFHCNGWGMPY
                     AVTAMGARQVIIRKIDGEEILRRVEAEGVTLFNCAPAVINAVLDAAGARRKRGEAVPG
                     KGRVRVVVAGAPPPSKTIERIESELGWEFIQIYGLTETSPLLTINRAPQEWDQLDPAE
                     RAKRLSRAGVPAVGVRMDVDASGEILARSNHVFEGYWNQPEESAKALADGWFHTGDGG
                     HAEGAYTVIADRKKDVIITGGENVSSIEVEDCLFQHPAVAEVAVIGVPDEKWGETIKA
                     LVVLRPGQQATERDLIEFTRGRMAHFKCPTAIEFRAELLRTATGKLQKYKLREPFWAG
                     RDRRVS"
     gene            complement(3408..3740)
                     /locus_tag="VNU03_00200"
     CDS             complement(3408..3740)
                     /locus_tag="VNU03_00200"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWP72892.1"
                     /translation="MDTQQQQRSRRVQSRAGVSWTAWVTSGSRRVRCHTVDLSANGAK
                     LKPRGEMQPGMSVDVQIHPPEGNPVHVSAVVWRVDSDSMALLFLKSLPVQTTGSRSWQ
                     ENGRRGWR"
BASE COUNT          598 a         1382 c         1426 g          555 t
ORIGIN      
        1 gtcgtcatca tcaggtggtg ggcccgggcc aggcgccggg tccggcggta ctcccgcagc
       61 gacatgccga ccacggcgtg gaaccgcgtc gagaaatacg agcggctcag cccggcccgc
      121 cgggccagat ccgggaggcg gcagcgggaa tcgtcgtgct cgtcgaggaa cgctcgcacc
      181 cgcttcatgg ccgtcgcccc gtgtcgcggg tcccggcgcg gcgcccggct caggatccct
      241 cggatggctc gcttgagcgt gggcccgggg gcgggatgcg ggaggaccgc ccggaccccg
      301 ctcatgagca cgtccgcccg caggcccgtg tgtgggcccg gatgagcgaa gacgagcacc
      361 gggatccacg gccagtacct gaagatgtcg gtcaagaggg tgaccccggc cacgtagggc
      421 gttccgctgg cctgggtcag tcccatgacg acgaccagat cgacacgccc ggtctgggcc
      481 cggaggaggg ccagcccgcg acgcaggccc tgggcctcga agatgtcgtg ctcgacttcg
      541 agcgcgcgga agacaccgcg ggacatccgc cgccccacat ggaccaccac gatgcgtcgt
      601 cggcgtctcg ggctgggcac gctcctcggt ccccaccgca tcgggagtcc cggtcgcccc
      661 gcacggcaga acccaagagc gtaagggcat ctccggcaat cccttctgga ggtgcctccc
      721 cgcctgaccg tcggtcccgg gagggacggt cagcatcctg acggtacacc cctgaacgtg
      781 cctccgggca gatagtttcc gtcccgccga cggcctccgc gacgtaccat cgaacaccgt
      841 gccgaccttt gcgggctacc cggcgagcga tgacccgagc ggcgccgcca tcgcgctgct
      901 cggcgtcccg gacgccaccc cgtatacccc gggacggtcc agccattccg cgggtgctcc
      961 ggccgcgatg cgcaccgcgc tgaccgggta cgcgacggcg ccggatcact acgatttcga
     1021 catcgacggc ctccgcccgt cgggggtcgt cgattgcggg gacctggtgg gagacccttc
     1081 cgatccgccg gggacgaggc agcgcatcac cgccgcgatc gcccggttcc tcgaggccgg
     1141 cgcggtgccg gtggtgctcg ggggcgacga ctcggtcgtg atccccttct tccaggcgtt
     1201 cgccggccgc ggcccactca ccgtcgtcca ggtcgatgcg catctcgact ggcgcgacga
     1261 ggtccgcggc gagcggttcg gatggagcag cccgatgcgg cgggcgtcgg agatgccctg
     1321 ggtggagcgc atggtccagg tcggcctgcg cggcatcggg agcgcccggg cgggcgaggt
     1381 cgcggacgcg cgggcgtggg gctcgtcgat cgtcaccgcc gccgaggtgc accgcgcggg
     1441 aatcggaatc gcagtcgacc gggtgccggc cggagcccgc tgcctggtca cgatcgactg
     1501 cgacgggctc gatcccgcgg tgatgccggg ggtgagcgca cgggccccgg gcgggctcct
     1561 ctaccatcac gtgctcgagc tcctgcatgg ggtcgccgcc aaggcgagca tcgtgggatt
     1621 cgacctcgtc gagctgatgc cggaccggga cccgagcggg atcgcggcgc tgaccgccgc
     1681 gcggctcgtg tgcaacggtc tcgcggccat cgcggtctcg cagggcgatc gagcgggagg
     1741 cccttgacgc ggccacgcgt ttcggccgat tctcgggtcg acaggaggcc gccatgatca
     1801 tcccgctgac cctggccgat ttcctcgagc gcgccgagct cgtctacggc gagcgcgagg
     1861 ccatcgtcga cgagcccaac ccgcccggcg gcgggctcgg ccgcatcacc taccggcgct
     1921 tcgcggcgat gtgccggagc atggccgcct cgctcgacga cctcggagtc ggcgagggcg
     1981 agcgggtggc catcgtctcg ccgaacgcgg cccgcttcct ggtgagcctc ttcggggtga
     2041 gcgtcttcgg ccgggtcctg gtgccggtga acttccggct caacaccgag gagatccgct
     2101 acatcatcga gcactccggc tccaccgtcg ccctgatcga ccccgagctg gaggagagcc
     2161 tgcgcccgat cccggtcaag caccgcatcg tcctgggcgc ggcgagcgac gcgcagctcc
     2221 tcgatcgccg cgacgcgcgc ccgcgcttcg gagtgtccga cgagaacgcg accgtctcca
     2281 tcaactacac ctcgggcacc accgcgcggc ccaagggcgt gcagctcacc caccggggct
     2341 gctggctcaa cgcggtgacg ttcggctggc acgtgggcgt gtccgaccgc gacgtgtacc
     2401 tgcacaccct gcccagcttc cactgcaacg gctggggcat gccctacgcg gtgaccgcga
     2461 tgggggcccg ccaggtcatc atccgcaaga tcgacggcga ggagatcctg cgccgcgtgg
     2521 aggccgaggg cgtcaccctg ttcaactgcg cgcccgcggt gatcaacgcg gtcctcgacg
     2581 cggcgggcgc ccgccgcaag cggggtgagg cggtgccggg caagggccgc gtgcgggtgg
     2641 tggtggccgg cgcgcccccg ccgtcgaaga ccatcgagcg gatcgagtcc gagctcggct
     2701 gggagttcat ccagatctac gggctcaccg agacgtcgcc gctgctcacg atcaaccgcg
     2761 cgccccagga gtgggaccag ctcgacccgg ccgagcgcgc gaagcggctc tcgcgggcgg
     2821 gggtccccgc ggtcggcgtg cgcatggacg tggacgcgag cggcgagatc ctcgcccggt
     2881 cgaatcacgt cttcgagggc tactggaacc agcccgagga atccgccaag gccctcgcgg
     2941 acgggtggtt ccacaccggc gacggcggcc acgcggaggg agcgtacacc gtcatcgcgg
     3001 accggaagaa ggacgtcatc atcaccgggg gcgagaacgt ctcgtcgatc gaggtggagg
     3061 actgcctctt ccagcacccc gcggtggcgg aggtggcggt gatcggggtg ccggacgaga
     3121 agtggggcga gaccatcaag gccctggtgg tcctgcggcc gggccagcag gccaccgagc
     3181 gcgacctgat cgagttcacg cgcggccgca tggcccactt caagtgcccg accgcgatcg
     3241 agttccgcgc cgagctgctg cgcaccgcga ccggcaagct ccagaagtac aagctgcgcg
     3301 agccgttctg ggccggtcgc gaccgccggg tgagctgagg cgccgggtct aggtcgcccc
     3361 gcgcatcatc tcacccgcgc atcacctcga agatcggctg ctcgggttta tctccagcct
     3421 cggcggccgt tctcctgcca cgacctgctg ccggtggtct gcaccgggag gctcttgagg
     3481 aacagcagcg ccatcgagtc cgaatccacg cgccagacga cggcggagac gtggaccgga
     3541 ttgccttccg ggggatggat ctgcacgtcc accgacatgc cgggctgcat ctcgccgcgc
     3601 ggcttgagct tggccccatt ggcgctgaga tcgaccgtgt ggcaacggac tcggcgactt
     3661 ccggacgtga cccatgcggt ccacgacacg cctgcacgag actgaacccg gcggctgcgt
     3721 tgctgctgtt gagtgtccat gggggtagac ggagagcaag ggtcgggcca gcgggaaata
     3781 cgaggggaaa cggccagtta gggtgaaagg tacgcaccgg atcgcttccc aggatggcga
     3841 cggcggggtg cattgtgggt aacgaaaggg acgattgaca gggcgcacgg ctcgggggca
     3901 acatcagcgc agaaattgcc gcccggcaag ggagaccatt caatgagcaa gacacgcccc
     3961 g
//
LOCUS       DATMCV010000008         5903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_266424, whole genome shotgun sequence.
ACCESSION   DATMCV010000008 DATMCV010000000
VERSION     DATMCV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266424"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VNU03_00205"
     CDS             <1..600
                     /locus_tag="VNU03_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HWP72893.1"
                     /translation="RLLDPQPGELVADTCAAPGTKTTHLAQLMANRGRIVAMDPNAAR
                     LRLLTQAASRLGVEIVETHAGGVATVSGRWKGRCDRVLVDAPCSNLGVLRRNPDVKWN
                     REETDLGRLVEKQRGILSAAALLARPGGRLVYATCSLEPDENDLIVRAFLDGHPGWRV
                     DPPADFPVAPDGTGFIRCLPQIHGTDGFTAVRLARAERS"
     gene            657..1316
                     /gene="rpe"
                     /locus_tag="VNU03_00210"
     CDS             657..1316
                     /gene="rpe"
                     /locus_tag="VNU03_00210"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWP72894.1"
                     /translation="MRIAPSILSADFAALGDAIARVEAAGADQLHVDVMDGHFVPNLT
                     IGPPVIESIRKRTRLPLDVHLMIEEPERWVETYVKAGADYLTIHVEACAHLERALSLI
                     REAGAKSGVALNPSTAPEVLEYVLDDLDLVLVMSVNPGFGGQSFIPTAYEKIRRLRAL
                     LGSRDVLVSVDGGVKVENAGPLARAGATVLVAGSSVFGAPDPGAAVAALRRASEVAKS
                     A"
     gene            1380..2723
                     /gene="ffh"
                     /locus_tag="VNU03_00215"
     CDS             1380..2723
                     /gene="ffh"
                     /locus_tag="VNU03_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWP72895.1"
                     /translation="MLDSLTSRLTGILDRLRGFGRLTEDNIQEALREVRVALLEADVN
                     FKVVKSFVERVRVKAVGQDVLQSLIPGQQVVKVVRDELVELLGGSAHRLAMASHPPTV
                     VMLIGLQGSGKTTSAAKLARHFQKQGQHPLLAAADVYRPAAIDQLKTLGAQLGVPVLG
                     EADQRPVDICSAARDEAARRGLSPLILDTAGRLHIDEEMLEELRAIKKAVGPHHVLLV
                     VDAMTGQDAVTVADKFNAAIGIDAVILTKMDGDARGGAALSVREVTGRPIAFVGVGEK
                     TDALEPFHPDRLAQRILGMGDVLSLVEKAQASVDAEQAEVLAQKIRDDAFTLEDFASQ
                     LKQLRSMGPLGQLMDMVPFFKGAKGLPKEMKGEEQELHRYEAIIASMTPHERREPAVI
                     NGSRRARIARGSGTSVSDVNRLLKQYAQLKKMMKGLKQMEGRMGKLKGALPFLPR"
     gene            2771..3040
                     /gene="rpsP"
                     /locus_tag="VNU03_00220"
     CDS             2771..3040
                     /gene="rpsP"
                     /locus_tag="VNU03_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWP72896.1"
                     /translation="MAVHIRLRRTGTTRKPAYRVVVADSRAARDGKFIEVLGHYNPLT
                     KPPTIKIQVERAQEWIKKGAQPSNTVKHLLARAAGAAAAGAAAKA"
     gene            3047..3571
                     /gene="rimM"
                     /locus_tag="VNU03_00225"
     CDS             3047..3571
                     /gene="rimM"
                     /locus_tag="VNU03_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWP72897.1"
                     /translation="MTDAPLVAVGEVLRPFGLRGELKVRPLTDRPRERFGRLDRCVLW
                     EPRGDRREDCRIASCRFEGETIFLRMDGVDSPEAARRFQGRLLAVERDEVLPLPEGQF
                     YPWQMEGAVVETREGRPVGRFVRVESGAQDLWVVEDDEHRQRLIPAVPSIVVEVSVAD
                     RRIVIDPPEGLLEL"
     gene            3568..4320
                     /gene="trmD"
                     /locus_tag="VNU03_00230"
     CDS             3568..4320
                     /gene="trmD"
                     /locus_tag="VNU03_00230"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWP72898.1"
                     /translation="MRIDIVTLFPAMLEVPLTQSILGRARTRGLVDVRVHDLREHAAG
                     RHRVTDEPPFGGGGGMILKPEPLAAAVDALRPGGAEARVILLGPAGRRFSQEVARELA
                     GRPRLILLCGRYEGVDERVCETVVDEELSVGDYVLSGGEPAALVVTDAIVRLLPGVLG
                     DEDAPARDSFARGLLEHPQYTRPEVFRGAAVPEVLRSGDHGRIARWRQLMSLWRTWRR
                     RPDLLETADLTPEEQKWTDGFRQGRTPSDFLD"
     gene            4345..4722
                     /gene="rplS"
                     /locus_tag="VNU03_00235"
     CDS             4345..4722
                     /gene="rplS"
                     /locus_tag="VNU03_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWP72899.1"
                     /translation="MEAIRIVEAGDLKKDRSGFKPGDTVKVMVKVVEGEKERLQAFEG
                     VVIRRRGGGTGASFTVRRISYGVGVERTFPVHSPRIDKIQVLKRATVRRSKLYYLRDL
                     VGKAARLKEKRIAVVPASGEQSS"
     gene            4732..5388
                     /locus_tag="VNU03_00240"
     CDS             4732..5388
                     /locus_tag="VNU03_00240"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWP72900.1"
                     /translation="MAPEAPYRFESAAWRRGVSRLAGVDEAGRGPLAGPVVAAAVVLA
                     PGERIRGVDDSKRLDPLERARLFEIIHARALGVGVAVVDHLTIDRINILQATRLAMAQ
                     ALGALALEPELVLTDFVEVPGLRCPQRNLVEGDRRSASVAAASIVAKVTRDRIMEEAD
                     REFPLYGFVRHKGYATPEHREALERHGPCPLHRRTFAGIWSQLTLFSRTELQWIDADA
                     "
     gene            5401..5781
                     /locus_tag="VNU03_00245"
     CDS             5401..5781
                     /locus_tag="VNU03_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015414971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWP72901.1"
                     /translation="MPTDTRKVTGDRGEEEAARFLARSGFAILDRNVRTRAGEIDLVA
                     KEGKTLVFVEVKTRRDMEGDPPQAAVNTRKQNRLGKLAHGYLKLKRLRQVSCRFDVVS
                     VIFNDEGAVKAIRHIPNAFSVSAW"
BASE COUNT          876 a         1978 c         2212 g          837 t
ORIGIN      
        1 cgcctgctcg atccacagcc cggagagctg gtggcggata cctgcgccgc gcccggcacc
       61 aagaccaccc acctggccca gctcatggcc aaccgaggcc ggatcgtggc catggacccc
      121 aacgcggccc ggctacggct cctgacccag gcggcgtccc ggctcggcgt cgagatcgtc
      181 gagacccacg ccggcggggt ggccaccgtg agcggccgct ggaagggacg gtgcgaccgg
      241 gtgctggtgg acgctccgtg ctcgaacctg ggcgtgctgc gccgcaaccc ggacgtgaag
      301 tggaaccgcg aggagacgga tctcgggcgc ctggtcgaga agcagcgagg catcctgtcg
      361 gccgcggcct tgctggcccg tccgggcggg cgcctcgtct acgccacctg ctcgctcgag
      421 cccgacgaga acgacctgat cgtgcgggcc ttcctggacg gtcacccggg ctggcgggtg
      481 gatccgccgg cggacttccc ggtagccccg gacgggaccg gattcatccg ctgcctgccc
      541 cagatccacg ggaccgacgg cttcaccgcg gtccgcctcg cccgcgcgga gcggtcctga
      601 ccgtcgctcg gcccgctccc tttgcgggcc ccgcgccttt cgggtaaggt cgcagcatga
      661 ggatcgcgcc cagcatcctg tccgccgact tcgccgccct gggggacgcg atcgcgcggg
      721 tggaggcggc cggggccgac cagctccacg tcgacgtgat ggacggccac ttcgtgccca
      781 acctcaccat cgggcccccg gtcatcgagt cgatccggaa gcgaacgcgg ctgccactcg
      841 acgtccacct gatgatcgag gagccggagc gctgggtcga gacctacgtc aaggcggggg
      901 ccgactacct gaccatccac gtcgaggcgt gcgcccatct cgagcgggcc ctctcgctga
      961 tccgggaggc gggcgccaag tccggggtgg ccctgaaccc ctcgaccgcg cccgaagtcc
     1021 tcgaatacgt gctcgacgac ctcgacctgg tgctggtgat gtcggtcaac ccgggcttcg
     1081 gggggcagtc gttcatccca accgcctacg agaagatccg tcgcctccgg gccctgctgg
     1141 gctcccgcga cgtgctggtc tcggtggacg gtggcgtgaa ggtcgagaat gccggtcccc
     1201 tggcccgggc cggggccacg gtgctggtgg ccgggtcctc cgtcttcggc gccccggatc
     1261 cgggggccgc ggtggcggca ctgaggcgtg cgtcggaagt agcgaaaagc gcttgacttt
     1321 tagggccctc gtcagtattc tttagcgttt ccagacgcag tcttccaggt ggtcacgcaa
     1381 tgctcgacag tctgaccagt cgcctgaccg gaatcctcga ccggctccgt ggtttcggcc
     1441 ggttgacgga ggacaacatc caggaggctc tgcgcgaggt gcgcgtggcc ttgctggagg
     1501 ccgacgtcaa cttcaaggtc gtgaagagct tcgtcgagcg cgtccgtgtg aaggcggtcg
     1561 ggcaggacgt gctgcagtcg ctcatcccgg gccagcaggt ggtcaaggtg gtgcgcgacg
     1621 agctggtcga gctgctcggc ggctccgccc accgcctggc catggcctcg cacccgccga
     1681 cggtcgtcat gctgatcggc ctgcagggct ccggcaagac caccagcgcc gccaagctgg
     1741 cccgccactt ccagaagcag ggccagcatc cgctgctggc cgccgccgac gtctaccggc
     1801 ccgccgcgat cgaccagctg aagaccctgg gcgcgcagct cggtgtcccg gtgctgggcg
     1861 aggccgacca gcgcccggtc gatatctgct ccgcggcgcg ggacgaggcg gcgcggcggg
     1921 gcctgtcgcc gctcatcctc gacaccgcgg ggcggctgca catcgacgag gaaatgctcg
     1981 aggagctgcg ggcgatcaag aaggcggtgg ggccgcacca cgtcctgctg gtggtggacg
     2041 cgatgaccgg ccaggacgcg gtcacggtgg ccgacaagtt caacgcggcc atcggcatcg
     2101 acgcggtgat cctcaccaag atggacggcg acgcgcgcgg cggtgcggcc ctgtcggtac
     2161 gggaagtgac cggccggccc atcgcgttcg tgggcgtggg cgagaagacc gacgcgctcg
     2221 agcccttcca tcccgatcgg ctcgcccagc gcatcctggg gatgggcgac gtgctctcgc
     2281 tggtggagaa ggcccaggcc tcggtggacg ccgagcaggc ggaggtcctc gcccagaaga
     2341 tccgggacga cgccttcacc ctcgaggact tcgcgtcgca gctcaagcag ctgcgctcca
     2401 tgggacctct cggccagctc atggacatgg tgcccttctt caagggggcc aaggggctgc
     2461 ccaaggagat gaagggcgag gagcaggagc tgcaccggta cgaggcgatc atcgcgtcga
     2521 tgaccccgca tgagcggcgc gagccggcgg tgatcaacgg cagtcgccgc gcgcggatcg
     2581 cgcggggcag cggcacgagc gtgtcggacg tgaaccggct gctcaagcag tacgcccagc
     2641 tgaagaagat gatgaagggg ctcaagcaga tggaagggcg catgggcaag ctcaagggcg
     2701 ccttgccctt cctgccgcgc tgagccctgg acgagtcgac gacgcaggac aacccagaga
     2761 gaggtgacca gtggcggttc acatcaggct gagacggacg gggacgacgc ggaagccggc
     2821 gtaccgcgtg gtggtggcgg actcgcgcgc ggcgcgggac ggcaagttca tcgaggtgct
     2881 cgggcactac aacccgctca ccaagccacc caccatcaag attcaggtgg agcgggccca
     2941 ggagtggatc aagaagggcg cgcagcccag caacaccgtg aagcacctgc tggcgcgggc
     3001 cgcgggggcc gccgccgcgg gagccgccgc caaggcgtag ccgatcgtga cggacgcccc
     3061 cctggtcgcg gtcggggagg tgctgcggcc cttcgggctg cgcggcgagc tcaaggtgcg
     3121 cccgctcacc gatcggcccc gcgagcgctt cggccggctc gaccgctgcg tgctgtggga
     3181 gccccgcggg gatcggcgcg aggactgccg catcgcctcg tgccgcttcg agggcgagac
     3241 gatcttcctg cgcatggacg gagtcgactc cccggaggcg gcgcgccgct tccaggggcg
     3301 gctcctggcg gtggagcgcg acgaggtgct gcccctgccc gaggggcagt tctatccctg
     3361 gcagatggag ggcgcggtgg tggaaacgcg cgagggacgg ccggtgggcc ggttcgtccg
     3421 cgtggagagc ggggcccagg acctgtgggt ggtcgaggac gacgagcacc gccagcggct
     3481 gatcccggcg gtgccgtcga tcgtggtgga ggtcagcgtg gcggatcgcc gcatcgtcat
     3541 cgatccgccg gaagggctgc tcgagctgtg aggatcgaca tcgtcaccct cttcccggcc
     3601 atgctcgagg tcccgctcac ccagtcgatc ctgggccggg cccggacccg cggcctggtc
     3661 gacgtccgcg tccacgacct gcgggagcac gcggcggggc ggcaccgggt gaccgacgag
     3721 ccgccgttcg gcggcggggg cggcatgatc ctgaagccgg agccgctggc cgccgcggtg
     3781 gacgcgctgc ggcccggtgg ggccgaggcg cgggtgatcc tgctggggcc ggccggccgg
     3841 cgcttcagcc aggaggtcgc ccgcgagctc gcggggcggc cgcgcctgat cctcctctgc
     3901 ggacgctacg agggcgtgga cgagcgggtg tgcgagacgg tggtggacga ggagctgtcc
     3961 gtcggcgact acgtgctgag cggcggggag ccggccgcgc tcgtggtgac cgacgcgatc
     4021 gtgcgcctcc tgccgggcgt gctcggcgac gaggacgcgc ccgcgcgcga ctcgttcgcg
     4081 cgcggcctgc tcgaacaccc gcagtacaca cggcccgagg tcttccgcgg cgcggcggtg
     4141 cccgaggtgc tgcgctccgg cgatcacgga aggatcgcgc ggtggcgcca gctgatgtcg
     4201 ctctggcgca cgtggcggcg gcggcccgat ctcctggaaa cggcggatct cacgccggaa
     4261 gagcagaagt ggacggacgg cttccggcag ggccggaccc cgtccgattt cctggactaa
     4321 cttcagagcg aggagcgtca gatcatggaa gcgatccgaa tcgtcgaggc gggcgatctc
     4381 aagaaggacc ggagcggctt caagcccggc gacaccgtca aggtgatggt caaggtcgtc
     4441 gagggtgaga aggagcggct gcaggccttc gagggcgtgg tgatccgccg gcgcgggggc
     4501 ggcaccgggg cctcgttcac ggtgcgccgc atctcctacg gggtcggggt ggagcgcacc
     4561 ttcccggtgc actcgccgcg gatcgacaag atccaggtgt tgaagcgggc caccgtgcgg
     4621 cggtcgaagc tctactacct ccgcgatctg gtcggcaagg cggcgcgcct caaggagaag
     4681 cgcatcgcgg tggtcccggc ctcgggcgag cagtcgagct aagaccgggt cgtggcgccg
     4741 gaagcgccct accggttcga gagcgccgcc tggcggcggg gggtgagccg tctcgccggc
     4801 gtcgacgagg cgggccgcgg gccgctcgcg gggccggtgg tggcggcggc ggtggtgctg
     4861 gcgccggggg agcggattcg cggggtcgac gactcgaagc gtttggaccc gctggagcgg
     4921 gcgcggctgt tcgagatcat tcacgcgagg gcactgggag tgggcgtggc ggtggtggat
     4981 cacctcacga tcgatcgcat caacatcctg caggccaccc gtctggccat ggcccaggcc
     5041 ctgggcgcgc tcgcgctcga gccggagctg gtgctgaccg acttcgtgga ggtcccgggc
     5101 ctgcggtgcc ctcagcggaa cctggtcgag ggcgaccggc gctcggcctc ggtcgcggcg
     5161 gcctcgatcg tggccaaggt gacgcgcgac cggatcatgg aagaagcgga tcgggagttc
     5221 ccgctctacg ggttcgtccg ccacaagggc tacgcgaccc cggagcaccg ggaggcgctc
     5281 gagcgtcacg gcccctgccc cctgcaccgg cgcaccttcg cggggatctg gagccagctg
     5341 accctgttct cccgtaccga gctgcagtgg atcgacgccg atgcgtaagg agtagtgcac
     5401 gtgcccaccg acactcgaaa ggtcaccgga gacagagggg aagaagaggc cgcccgcttc
     5461 ctggcccgca gcggctttgc gatcctggac cgcaacgtcc gcacccgcgc gggggagatc
     5521 gacctcgtgg cgaaggaggg caagaccctc gtcttcgtcg aggtgaagac gcgccgggac
     5581 atggagggcg atccgcccca ggccgcggtc aacacccgca agcaaaaccg gctcggaaag
     5641 cttgcccacg gctatctcaa gctcaagcgg ctgcggcagg tctcctgccg cttcgacgtc
     5701 gtctcggtga tcttcaacga cgagggcgcg gtcaaggcga tccggcatat tccgaacgcg
     5761 ttctccgtgt ccgcctggta gcgccgatgg atcgggagga ggcgttcgcc cgcgccctgc
     5821 cgctcgagcc gggccgcatc gcgctcctcg tcgtcgacat gcagcgcggg ttcgtggaag
     5881 agggccaggt catggaggtc ccg
//
LOCUS       DATMCV010000009         6781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_931075, whole genome shotgun sequence.
ACCESSION   DATMCV010000009 DATMCV010000000
VERSION     DATMCV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931075"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VNU03_00250"
     CDS             complement(<1..471)
                     /locus_tag="VNU03_00250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP72902.1"
                     /translation="MKYGLHGVNLNTCAYPDAAARLGRAAEAAGFESLWVADHVVLPD
                     PPVAGRPMAPDMRLLDPVVALTFLAAHTSRIRLGTGVIILPQRQALVLAKQLASLDVL
                     SNGRLIFGLGVGWCEPEMRSVGAPFKERGRVSDDYLASMRAVWTQPKPSHHGPYV"
     gene            complement(468..1373)
                     /locus_tag="VNU03_00255"
     CDS             complement(468..1373)
                     /locus_tag="VNU03_00255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP72903.1"
                     /translation="MALSVGFIGVGNMGNPMAGNILKAGFPMAVFDLNPKAMANLVEA
                     GARRADSALAALEGADVVLTSLPASPDVEAMYLDAGGLVERAKPGTILIDLSSVLPST
                     PRKIEPRAKARGVHFLEAPVSGGVTGARAATLAIMVGGDKAALSRAEPVLKAMGPNVF
                     HVGPAGAGNTVKAINNMMACVNSATMMEGFVLGVKAGLDPMTIYAVVKASSGGSKALE
                     RIPRALIPRDFEPGFKVALMNKDLETFHTIAKELHVPVTFSALAQHYQQAALAAGLGE
                     QDTSVVFTIIERLAGMKPPTPREPR"
     gene            1477..2199
                     /locus_tag="VNU03_00260"
     CDS             1477..2199
                     /locus_tag="VNU03_00260"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP72904.1"
                     /translation="MLRAFALDPIGTVSIEEMSSVGLLKLSTRTYWRRVWPAGVGLAS
                     YIVERFGTEGLRDRDVLDLGCGVGLVGIVCGRLGARVTFLDREAGALAAVRRNCRRNG
                     LAPARTIGGDWNHGGHRVEPDAYDLVVGGDVVYDDLEWPAIGTALMRTLRAGGIALLA
                     DPGWVVQRKLGGAFRRSGFTVARSRRRVSWPPWRTTHLRTKDINIYELSRRATPRPTL
                     SPQGRGQGEGLQGLTPQARPTG"
     gene            complement(2171..2500)
                     /locus_tag="VNU03_00265"
     CDS             complement(2171..2500)
                     /locus_tag="VNU03_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72905.1"
                     /translation="MREPGSPDLPDYSDPLDWRTGRSEALGPGHGQVARVVVRDGLVG
                     AGRVGALAFGALALGAFAIGALSIGRLVVRSLKLGRGEIRSLKIADLTVGTLRVGRIT
                     RSDAPEE"
     gene            complement(2509..3333)
                     /locus_tag="VNU03_00270"
     CDS             complement(2509..3333)
                     /locus_tag="VNU03_00270"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP72906.1"
                     /translation="MLIVDAQVHIWANHKPTNANHRQVETYSADDLLAEMDEAGVHGA
                     VIHPPASWDPDSNELAVEAARRHPDRLAILGNFPLDAPASRGLVDGWKKRPGMLGLRF
                     ALLQPHQRTWPTDGTMDWLWPAAERAGLPIALLAATYLPTVGQIAERHPRLKLIIDHL
                     GRPSGTKDDAAWANLPEMLALAKHPNIAIKATGAPSYSSAPYPYRNIHDHLRRIHDAF
                     GPERMFWGTDITRMPCPWRQCVTMFTEELPWLEGRALELVMGRAVCDWLDWRLPPR"
     gene            complement(3357..4544)
                     /locus_tag="VNU03_00275"
     CDS             complement(3357..4544)
                     /locus_tag="VNU03_00275"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP72907.1"
                     /translation="MNGNGALPLARFTVLDLTRARAGPTCVRQLVDWGARAIKIEMPG
                     GRSGDGMGGNRHGFDFQNLHRGKEALTLNLREPDGVAVFKRLAKDADIVVENYRPDVK
                     RRLGIDYESLQPLNPRLIYGSISGFGQSGPYRDRPGVDQIAQGMGGLMSIIGIPGQGP
                     VRVGIPIADLCSGILLAQGILVALIERESTGQGKWVHTSLLEAMLSMLDFQASRWLMS
                     GEVPPQAGNNHPTGIPTGVFETKDGHINIAAAGDELYERFCGAIERPDLRSDPRFATA
                     KARSTNRDLLMEVLMPVTRQKDSATWIQILNDAGVPCGPIYRIDEAFADPQVRHLEMA
                     QPVRSPALGDLTILGHPVSHGARRNPIRAAAPELGQDNEAILGSLGYSTADIADLAKR
                     GII"
     gene            complement(4541..5476)
                     /locus_tag="VNU03_00280"
     CDS             complement(4541..5476)
                     /locus_tag="VNU03_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72908.1"
                     /translation="MLGLGLASSHAPMMFQKAQYWPKVVERIPKVAREHLPQSARVEI
                     ATPSIIEGHIQKIEAAFAVLRDQVKAYRPDVLVMIGDDQGDMFDDANNPTFSIYTGDE
                     PIWGRSARDPMTTPPQERTKLVFPPHAEMSRYLLKGLIKRGFDVANVGKFDPRGNPTR
                     GVSHMVSNLVPEVDPELRIPLVCVFLNEYFPPLPSAARCAQLGAAIGQILNERPERIA
                     IYASGGLSHFPGMFNAGWIDQPLDRWILERLERNDLAALEHLFTFDSDTMRSGTGEVR
                     AWISAAAAMNRPAKFVEYTAAHCTQTGCGFVYWPA"
     gene            5559..6653
                     /locus_tag="VNU03_00285"
     CDS             5559..6653
                     /locus_tag="VNU03_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HWP72909.1"
                     /translation="MILERFKVPEKDQVRVSEAALREAVTRIFEKLGVSPEDAADAAD
                     VLTMTDLRGVETHGVSNMLRAYVRDYRAGKLDPRPGWRIEREAPATAVIDAGKRLGII
                     VGPKAMRLAIEKARRVGVGVVTVYNSGHFGAIGHFAMQAVQEDMVGVCFTAAGLHVVP
                     TFGAKPLLGTNPIAVAAPARQEAPLLFDAATSAIAGNKIRLAMRIGSPLLAGWVTDKE
                     GTPIMEEKPIFDREDFYQAPLGGTREQGSHKGYGFAMMAEVLSTFLAGALPTMLDPAS
                     GSKNQFAAYDIKAFTDLDRFKDTMDEMLRTLRTAAPAPGQERVLYPGLSEAEEVEHRR
                     AHGIPLHREVIEWFGECTREMGLPPLAVMS"
BASE COUNT          966 a         2383 c         2434 g          998 t
ORIGIN      
        1 cacgtagggc ccgtggtgcg acggcttcgg ctgcgtccac accgcacgca tcgaggccag
       61 gtagtcgtcg gagacacggc cgcgctcctt gaacggggcg cccaccgagc gcatctccgg
      121 ctcgcaccag ccgaccccga gcccgaagat gaggcgcccg ttcgagagca cgtcgagcga
      181 ggcgagctgc ttggccagca ccagggcctg gcgctgaggc aggatgatca cgcccgtccc
      241 cagccggatg cgcgaggtgt gggcagccag gaaggtcagc gccaccaccg gatcgagcag
      301 ccgcatgtcg ggcgccatcg gccgcccggc caccggcgga tcgggcagca ccacgtgatc
      361 ggccacccag agcgactcga agccggcggc ctcggcggcg cggccgagtc gcgccgccgc
      421 gtccggatac gcgcaggtgt tgaggttgac gccgtgcagg ccgtacttca tcgcggctct
      481 cgcggcgtcg gcggcttcat gccggccagc cgttcgatga tcgtgaacac cacgctcgtg
      541 tcctgctcgc cgaggccggc ggccagcgcg gcctgctggt agtgctgggc cagggccgag
      601 aaggtcaccg gcacgtgcag ctccttggcg atggtgtgga aggtctcgag gtccttgttc
      661 atgagcgcca ccttgaagcc gggctcgaag tcgcgcggga tcagggcgcg cgggatgcgc
      721 tcgagggcct tgctgccccc gctgctggcc ttcaccaccg cgtagatcgt catcgggtcg
      781 aggcccgcct tcaccccgag cacgaagccc tccatcatgg tggccgagtt cacgcacgcc
      841 atcatgttgt tgatcgcctt cacggtgttg cccgcgccgg ccgggcccac gtggaagacg
      901 ttgggcccca tcgccttcag caccggctcc gcccgggaga gcgcggcctt atcgccgccc
      961 accatgatgg ccagggtcgc ggcgcgggcc cccgtcacgc caccgctcac cggcgcctcc
     1021 aggaagtgga cgccccgcgc cttcgcccgg ggctcgatct tccggggcgt ggagggcagc
     1081 acgctcgaga gatcgatcag gatggtgccc ggcttggccc gctccaccag gccgcccgcg
     1141 tcgaggtaca tcgcctccac gtccggcgag gcgggcagcg aggtcagcac gacgtccgcg
     1201 ccctccagcg cggccagcgc cgagtcggcg cggcgggccc cggcctcgac caggttcgcc
     1261 atcgccttgg ggttcagatc gaagaccgcc atcgggaacc cggccttcag gatgttgccg
     1321 gccattggat tgcccatgtt gccgacaccg atgaacccga ccgagagagc catgcgcgcc
     1381 tccttgggcg acctgcggga tgacgggggt cgaatcgaac gcgccgagca taaacctccg
     1441 cgacgagcgg agcaacgtgc ggtagactcc gctctagtgt tacgcgcctt cgcgctcgac
     1501 ccgatcggga cggtctccat cgaggagatg tcgtcggtgg gtcttctgaa gctctcgacg
     1561 cggacgtact ggcgccgcgt ctggcccgcc ggggtcggcc tggcctccta catcgtggag
     1621 cgcttcggca ccgagggact gcgcgaccgc gacgtcctgg atctcggctg cggggtaggc
     1681 ctggtcggca tcgtgtgcgg ccgcctgggc gcgcgcgtca ccttcctcga ccgcgaggcg
     1741 ggagccctcg ccgcggtccg gcgcaactgc cgccgcaacg gcctggcccc ggcccgcacc
     1801 atcggcggag actggaacca cggcggccac cgggtcgagc cggacgccta cgatctggtc
     1861 gtcggcggcg acgtggtcta cgacgacctg gagtggccgg ccatcggcac cgcgctgatg
     1921 cggaccctgc gggcgggcgg catcgcgctg ctggccgatc cgggctgggt ggtccagcgc
     1981 aagctgggtg gcgcattccg ccgcagcggc ttcacggtgg cccggagccg gcggcgggtg
     2041 agctggccgc cgtggcggac cacccacctg cgcaccaagg acatcaatat ctacgagctg
     2101 tcccgccgcg ccacccctcg tcccaccctc tcccctcagg ggagagggca gggtgagggt
     2161 ctccaagggc tcactcctca ggcgcgtccg accgggtgat acggcccacg cggagggtcc
     2221 cgaccgtgag gtcggcgatc ttcagcgagc ggatctcacc ccgcccgagc ttcaagctcc
     2281 gcaccaccag gcgcccgatg gagagcgcgc cgatcgcgaa ggctccgagc gccagcgccc
     2341 cgaaggccag cgcccccacc cggccggccc cgaccagccc gtcccggacc acgacccgcg
     2401 ccacctgacc gtgccccggg ccgagggcct cggagcggcc cgtcctccag tcgaggggat
     2461 cggagtagtc ggggaggtcg ggtgacccgg gctcgcgcat ggtgagggtc agcgcggggg
     2521 cagccgccag tcgagccagt cgcagaccgc gcggcccatg accagctcaa gggcccgccc
     2581 ctcgagccag ggcagctcct cggtgaacat ggtcacgcac tgccgccacg ggcacggcat
     2641 gcgggtgatg tcggtgcccc agaacatccg ctccggcccg aaggcgtcgt ggatgcgacg
     2701 caggtggtca tggatgttcc ggtatggata gggcgcgctc gagtagctgg gtgccccggt
     2761 ggccttgatc gcgatgttgg ggtgcttggc cagcgccagc atctcgggca ggttggccca
     2821 cgccgcgtcg tccttggtgc cggagggccg gcccaggtga tcgatgatca gcttgagccg
     2881 cggatgacgc tccgcgatct ggcccaccgt gggcaggtag gtcgccgcga ggagcgcgat
     2941 cggcaggccc gcgcgctccg ccgcgggcca gagccagtcc atcgtcccgt cggtcggcca
     3001 cgtgcgctga tgcggctgca gcagcgcgaa gcgcagcccg agcatccccg gccgcttctt
     3061 ccagccgtcg accagccccc ggctcgcggg ggcgtcgagc gggaaattgc cgaggatcgc
     3121 caggcgatcc gggtggcggc gcgctgcctc caccgccagc tcgttggagt ccgggtccca
     3181 cgaggcgggc gggtggatga ccgcgccgtg gacgccggcc tcgtccatct cggccagcag
     3241 atcgtcggcg gagtaggtct cgacctgccg gtggttcgcg ttggtgggct tgtggttggc
     3301 ccagatgtgg acctgcgcgt cgacgatcag catgggaggc cccgggggtc agacggtcag
     3361 atgatgccac gcttggccag atcggcgatg tcggccgtgc tgtagccgag cgatccgagg
     3421 atggcctcgt tgtcctggcc aagctcgggg gcggcggcgc gaatggggtt gcgccgcgct
     3481 ccgtgcgaga ccgggtgccc gaggatcgtg aggtcgccga gcgcgggcga gcggaccggc
     3541 tgcgccatct cgaggtggcg cacctgcgga tcggcgaagg cctcgtcgat ccggtagatg
     3601 ggcccgcacg gcacgcccgc gtcgttcagg atctggatcc aggtcgcgct gtccttctgc
     3661 cgcgtgaccg gcatcaggac ctccatcagg aggtcgcgat tggtcgagcg cgccttcgcg
     3721 gtcgcgaagc gcggatcgct gcgcaggtcc gggcgctcga tcgcgccgca gaaccgctcg
     3781 tacagctcgt cgcccgccgc cgcgatgttg atgtggccgt ccttggtctc gaagacgccg
     3841 gtcgggatcc cggtgggatg gttgttgccg gcctgcggcg ggacctctcc gctcatgagc
     3901 cagcgcgagg cctggaagtc gagcatggag agcatcgcct cgagcagcga ggtgtgcacc
     3961 cacttcccct gcccggtgct ctcgcgctcg atcagggcca ccaggatgcc ttgcgcgagc
     4021 aggatgcccg agcagagatc cgcgatcggg atgccgaccc gcaccggccc ctgccccggg
     4081 atgccgatga tcgacatgag gccgcccatg ccctgggcga tctggtccac cccgggccga
     4141 tcgcgatagg gcccgctctg gccgaagccc gagatgctgc cgtagatgag ccgcgggttg
     4201 agcggctgca ggctctcgta gtcgatgccg agccggcgct tgacgtccgg gcggtagttc
     4261 tcgaccacga tgtcggcgtc cttcgccagc cgcttgaaga ccgccacccc gtcgggctcg
     4321 cgcaggttca gcgtcagggc ttccttgccg cggtgcaggt tctggaagtc gaacccgtgg
     4381 cggttgccgc ccatcccatc gcccgagcgg ccgcccggca tctcgatctt gatcgcgcga
     4441 gcgccccagt ccaccagctg acggacgcag gtcgggccgg cccgcgcccg ggtgagatcg
     4501 agcacggtga agcgcgccag gggcagcgcg ccgttgccgt tcatgccggc cagtagacga
     4561 acccgcagcc ggtctgcgtg cagtgggccg cggtgtactc cacgaacttg gccggccggt
     4621 tcatcgcggc ggccgcgctg atccatgccc gcacctcgcc ggtgccggag cgcatcgtgt
     4681 ccgagtcgaa ggtgaacagg tgctcgaggg cggcgaggtc gttgcgctcc aggcgctcga
     4741 ggatccagcg gtcgagcggc tggtcgatcc agcccgcgtt gaacatgccc gggaagtggg
     4801 agaggccgcc cgaggcgtag atcgcgatgc gctcgggccg ctcgttcagg atctggccga
     4861 tcgccgcgcc cagctgggcg cagcgcgcag cgctcggcag cggcgggaag tactcgttga
     4921 ggaagacgca gaccagcggg atgcgcagct ccggatccac ctcgggcacc aggttcgaca
     4981 ccatgtgcga gacgccgcga gtcgggttgc cgcgcggatc gaacttcccc acgttggcga
     5041 cgtcgaagcc gcgcttgatc aggcccttca ggaggtagcg cgacatctcg gcgtgcgggg
     5101 ggaagaccag cttggtccgc tcctgcggcg gcgtcgtcat ggggtcgcgc gcgctccggc
     5161 cccagatcgg ctcgtccccg gtgtatatcg agaaggtcgg gttgttggca tcgtcgaaca
     5221 tgtcgccctg gtcgtcgccg atcatgacca gcacgtccgg gcggtaggcc ttcacctggt
     5281 cgcgcagcac cgcgaaggcg gcctcgatct tctggatgtg gccctcgatg atgctgggcg
     5341 tcgcgatctc cacccgggcg ctctgcggga ggtgctcccg ggccaccttg gggatccgct
     5401 cgacgacctt gggccagtac tgcgccttct ggaacatcat gggggcgtgc gacgaggcca
     5461 aacccagtcc gagcatggtg gagtcctccc gcctggaaat ctagcacgcc cgtgggccta
     5521 tgcaatcggc taggattccg ggcgggagga ggcgacccat gatcctcgag cgcttcaagg
     5581 tgccggagaa ggaccaggtc cgggtgtcgg aggcggccct ccgcgaggcc gtcacccgga
     5641 tcttcgagaa gctcggcgtg agcccggagg acgcggcgga cgcggccgac gtgctcacca
     5701 tgaccgacct gcgcggggtc gagacccacg gcgtgtccaa catgctgcgc gcctacgtcc
     5761 gcgactaccg ggccggcaag ctcgacccgc gcccgggctg gcgcatcgag cgcgaggcgc
     5821 ccgccaccgc ggtgatcgac gcgggcaagc ggctcggcat catcgtgggg ccgaaggcga
     5881 tgcgcctggc catcgagaag gcgcgccggg tcggcgtcgg ggtggtgacg gtgtacaaca
     5941 gcgggcactt cggcgccatc ggccacttcg cgatgcaggc cgtgcaggag gacatggtcg
     6001 gggtctgctt caccgccgcc ggcctccacg tcgtccccac cttcggcgcc aagcccctgc
     6061 tcggcaccaa tccgatcgcg gtggccgccc ccgcgcggca agaggccccg ctgctcttcg
     6121 acgcggccac ctccgccatc gcgggcaaca agatccggct cgcgatgcgg atcggctcgc
     6181 cgctgctggc aggctgggtg accgacaagg agggcacgcc gatcatggag gagaagccga
     6241 tcttcgaccg cgaggacttc taccaggccc cgctcggcgg cacccgggag cagggctcgc
     6301 acaagggcta cggcttcgcg atgatggcgg aggtgctctc gaccttcctg gccggggcgc
     6361 tgcccaccat gctcgacccg gccagcggct ccaagaacca gttcgcggcc tacgacatca
     6421 aggccttcac cgacctcgac cgcttcaagg acacgatgga cgagatgctc cgcaccctgc
     6481 gcaccgccgc gcccgcgccg gggcaggagc gggtgctcta tccgggactg tccgaggcgg
     6541 aggaggtcga gcatcgccgg gcccacggca tcccgctcca ccgggaagtg atcgagtggt
     6601 tcggcgagtg tacccgggag atgggcctgc cgcccctggc cgtcatgagc tgagccgcgg
     6661 ccgggcgggc ccgctctgat cgaccagggc ctgggccaac ggttccagag actcggctga
     6721 tacttctggc cgtctctcag cggccatgcc cgcctccgcc gtgcccgcct ccgccgccgt
     6781 g
//
LOCUS       DATMCV010000010        11934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_532488, whole genome shotgun sequence.
ACCESSION   DATMCV010000010 DATMCV010000000
VERSION     DATMCV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11934
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532488"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..287
                     /gene="rpmC"
                     /locus_tag="VNU03_00290"
     CDS             81..287
                     /gene="rpmC"
                     /locus_tag="VNU03_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWP72910.1"
                     /translation="MKAAKWREMSDEEVQQKAQELGEELFNLRFQLSMGVAKNPARVA
                     IARRDLARAHTILRERALRIPRTA"
     gene            308..556
                     /gene="rpsQ"
                     /locus_tag="VNU03_00295"
     CDS             308..556
                     /gene="rpsQ"
                     /locus_tag="VNU03_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWP72911.1"
                     /translation="MGVKKTREGVVVSDKMTKTRVVVIERVFRHPQYERVITRSKRLK
                     AHDEKNASKVGDRVLIEETRPLSKEKRWRILQVLASAS"
     gene            553..921
                     /gene="rplN"
                     /locus_tag="VNU03_00300"
     CDS             553..921
                     /gene="rplN"
                     /locus_tag="VNU03_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWP72912.1"
                     /translation="MIQPRSMLDVADNSGAKKVQCIRVMGGANKRYASLGDVVVVAVK
                     EAVPDGTVKKGEVARAVVVRTVKEVGRRDGSYIRFDRNAVVLLKADDNPVGTRIFGPV
                     ARELRDKQFTKIISLAPEVI"
     gene            932..1258
                     /gene="rplX"
                     /locus_tag="VNU03_00305"
     CDS             932..1258
                     /gene="rplX"
                     /locus_tag="VNU03_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWP72913.1"
                     /translation="MAIAHVRKGDTVMVVAGKERGKKGKVLRVIPEKGRVVVERLNLI
                     KKHQKPTQKIRQGGIIEREGSIHLSNVMLIDPGSDKPTRVGMKALSDGKKVRVARRSG
                     EMLDRA"
     gene            1440..1973
                     /gene="rplE"
                     /locus_tag="VNU03_00310"
     CDS             1440..1973
                     /gene="rplE"
                     /locus_tag="VNU03_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWP72914.1"
                     /translation="MRERFRSAVVPAMMKERGYGNPFQVPRLEKVVINMGVGEGKENA
                     KVLDFATADLQAIAGQKPVITRAKKSIANFKLRENVPIGCKVTLRGPRMYEFLDRLVN
                     VALPRVRDFKGVPPKGFDGRGNYNMGLKEQIIFPEIVYDKVDKVRGMDITMVTTARTD
                     EEAKALLTHLGVPFREA"
     gene            1974..2159
                     /locus_tag="VNU03_00315"
     CDS             1974..2159
                     /locus_tag="VNU03_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HWP72915.1"
                     /translation="MAKTALIMKQQRPPKFKSRAYTRCKICGRPRGYLRKFQACRLCF
                     RELALKGEVPGVIKASW"
     gene            2164..2556
                     /gene="rpsH"
                     /locus_tag="VNU03_00320"
     CDS             2164..2556
                     /gene="rpsH"
                     /locus_tag="VNU03_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWP72916.1"
                     /translation="MMSDPIADFLTRIRNASRAEHEKVDIPSSKLKLRMAEILKDEGF
                     IKNFRVIEDNKQGVLRVYLKYAGTEKIISGIVRVSRPGRRIYVTKDKIPTILGGMGVA
                     ILSTSAGVMTDRQVKKQHLGGEVLAYVW"
     gene            2569..3111
                     /gene="rplF"
                     /locus_tag="VNU03_00325"
     CDS             2569..3111
                     /gene="rplF"
                     /locus_tag="VNU03_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWP72917.1"
                     /translation="MSRIGKKPISVPQGVKVALDGAIVRAEGPKGKLSQAIPSGVSVT
                     MDSNVLTVARSSDHRNVRALHGLTRSLVANMIHGVKDGFERKLEIVGIGYRCQLQGKA
                     LQLALGYSHPVIFPLPDGIQAEVERQVSITLRGADKALLGQTAAKLRALRKPDPYKGK
                     GIKYSDEYIRRKVGKKAGAK"
     gene            3111..3476
                     /gene="rplR"
                     /locus_tag="VNU03_00330"
     CDS             3111..3476
                     /gene="rplR"
                     /locus_tag="VNU03_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWP72918.1"
                     /translation="MLTGKKVGGRKIRHLRIRTRVIGTAERPRLAVFRSLNHIYAQVI
                     DDTSGRTLAAVDSRAPDFRQKVKAGGNVAAAKIVGELLAERAKARGISQVVFDRGGYQ
                     YHGRVKALADAARAAGLGF"
     gene            3490..4020
                     /gene="rpsE"
                     /locus_tag="VNU03_00335"
     CDS             3490..4020
                     /gene="rpsE"
                     /locus_tag="VNU03_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWP72919.1"
                     /translation="MAEARIDANALDLTDRVVAINRVAKVVKGGRRFSFTALVVVGDG
                     QGHVGVGLGKAREVPEAIRKSVEHAKKDLVKVPLKDTTIPCEITGHFGAARVYLKPAA
                     QGTGVIAGGAVRPVLEAAGVQDILTKTLGTNNPHNVLKATMDAFRRLKRLQSTYAARR
                     RSLDGDAQETAGAKEA"
     gene            4004..4186
                     /gene="rpmD"
                     /locus_tag="VNU03_00340"
     CDS             4004..4186
                     /gene="rpmD"
                     /locus_tag="VNU03_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWP72920.1"
                     /translation="MPKKLKVTLVRSIIGMSPTQEATVKALGLRRIRHQVEHEDTPTI
                     RGMIATVPFAVEVQES"
     gene            4183..4659
                     /gene="rplO"
                     /locus_tag="VNU03_00345"
     CDS             4183..4659
                     /gene="rplO"
                     /locus_tag="VNU03_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWP72921.1"
                     /translation="MRLEDLRPAPGSTKNRKRLGRGRGSGQGKTSGKGHKGLNARSGG
                     GARPGFEGGQMPLYRRLPKRGFLPYGGKTEFAIVNVGDLSDRFAAGSVVDPDALAGSR
                     LIHKSGRASVKVLGDGDVAHALTVRAHKVSEGAKQKIEAAGGRVEILAARIARAAK"
     gene            4659..5966
                     /gene="secY"
                     /locus_tag="VNU03_00350"
     CDS             4659..5966
                     /gene="secY"
                     /locus_tag="VNU03_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWP72922.1"
                     /translation="MEGLQSFQNIFKVPELKRRVLMTLVLLAAYRLGAHVPTPGIDAA
                     ALAEFFNQVQGTLLGMVDLFSGGNLRRLTVFALGIMPYISASIILQLLTVVIPALERL
                     AKEGEAGKKKITQYTRYGTIALSLVQSFGIAVGLESMRSSTGAVLVPDPGWGFRLLTM
                     LTLTTGTALIMWLGEQISEKGIGNGISLIIFAGIVVRLPSAIVSSYTLITTGELQLFV
                     FLGLIIMMVAVTAAVVVMQEGQRKIPVQYAKRVVGRRMYGGQSTHIPLRINTAGVIPV
                     IFASSLILFPATLTRFIHHPWMQYVSEALSPGHVTYTALYCALIIFFAYFYTAIVFNP
                     VDLADNMKKYGGFIPGVRPGKKTAEYIDRVLTRITLPGAIFLAMISVLPDFLIRWFNT
                     PFYFGGTSLLIVVGVALDTVRQMESHLLMRNYEGFLRKKAKGA"
     gene            5963..6622
                     /locus_tag="VNU03_00355"
     CDS             5963..6622
                     /locus_tag="VNU03_00355"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWP72923.1"
                     /translation="MSRVIFLGAPGAGKGTQARRLAGESGVPQVATGDMLREAVAEGT
                     PLGRQAKRYMDSGALVPDDVVIGLVDERLARPDAGGGYVLDGFPRTVAQAEALDALLG
                     RRRQELDRVIFFDVSRDELIRRLTGRRICRKCGTAFHLVSAPPKAAGRCDQCGGELYQ
                     REDDAEATVARRLDVYQTQTAPLLDYYRQRGLLARVAGEGPVEEVADRIRKAVKEPVS
                     R"
     gene            6619..7380
                     /gene="map"
                     /locus_tag="VNU03_00360"
     CDS             6619..7380
                     /gene="map"
                     /locus_tag="VNU03_00360"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWP72924.1"
                     /translation="MILLKSARELAHMRTAGRVLAEVKDRLRSLVRPGISTKEIDTDV
                     EAFIVSRGATSAFKGYRGFPATVCISINEEVVHGIPSPKRKLREGDIVGLDLGCIIEG
                     YYADCAITLPVGAVPPRVQELLDVTRESLDKAIVQCRAGNRLGDVSHAVQEHCESHGF
                     GVVRAFVGHGIGQSLHEEPQIPNFGEAGRGPVIKAGMVLAIEPMVTMGSWEVRVLEDR
                     WTAVTVDGSWAAHFEDTIAITERGPEVLTRLDQGA"
     gene            7391..7609
                     /gene="infA"
                     /locus_tag="VNU03_00365"
     CDS             7391..7609
                     /gene="infA"
                     /locus_tag="VNU03_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWP72925.1"
                     /translation="MAKEDAIEVEGTVVEPLPNAMFRVELENGHKVLAHVSGKMRMNF
                     IRILPGDKVKVELSPYDLTRGRITYRFK"
     gene            7639..7752
                     /gene="rpmJ"
                     /locus_tag="VNU03_00370"
     CDS             7639..7752
                     /gene="rpmJ"
                     /locus_tag="VNU03_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWP72926.1"
                     /translation="MKVRPSIKVRCENCKIVRREGIVRIICKNPRHKQRQG"
     gene            7761..8165
                     /gene="rpsM"
                     /locus_tag="VNU03_00375"
     CDS             7761..8165
                     /gene="rpsM"
                     /locus_tag="VNU03_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWP72927.1"
                     /translation="MARIAGVDLPRDKRGQIAVQYIYGIGIPSAKKIFADARVDPNKR
                     VKTWSDDETGRVRDIIEREYKVEGDLRREVSMSVKRLMDIGCYRGIRHRKGLPVRGQR
                     THTNARTRKGKSKGVMVKKKPVAAAPSAPPKK"
     gene            8178..8600
                     /gene="rpsK"
                     /locus_tag="VNU03_00380"
     CDS             8178..8600
                     /gene="rpsK"
                     /locus_tag="VNU03_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWP72928.1"
                     /translation="MADAAEQKPDEKAAPRKGGRKRERKEVRVGIAHVQATFNNTIVT
                     LTDKMGNVVSWASAGSVGFKGSRKSTPFAAQTAAENAARKAMEVGLKQVEVFVRGPGA
                     GREAAIRSLQAVGLEITAIRDVTPIPHNGCRPPKRRRV"
     gene            8613..9236
                     /gene="rpsD"
                     /locus_tag="VNU03_00385"
     CDS             8613..9236
                     /gene="rpsD"
                     /locus_tag="VNU03_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWP72929.1"
                     /translation="MARYRAAVCRLCRREGIKLFLKGSRCFTEKCAIERRNYPPGQHG
                     LGRTKPTQYGIQLREKQKAKRIYGVLEAQFRRYFAWAEADKGITGENLLKYLERRLDN
                     VVFRLGMAASRREARQMIGHGHVQVNGRKVSIPSFIVKVGEVVQLRSTSKMQERVADN
                     LTAGRSPVPPWLDLDVNEKKGTVRGLPLREDIQIPVQEQLIVELFSK"
     gene            9263..10249
                     /locus_tag="VNU03_00390"
     CDS             9263..10249
                     /locus_tag="VNU03_00390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWP72930.1"
                     /translation="MARIPFQKPKSIQWEIRSDRYGRLVAEPFEKGYALTVGHSLRRT
                     LLAIVPGSAVAWVRIPGVKDLDTRIPGVQEDTTDVLLNVKKLAVNVPSGEPTETRLEA
                     KGPKTVTGADVSEATGIEVLNPEIHIATLGAGGSLSMDLGIGVGRGYVAADRHPAGTV
                     PAGAIPLDAAFSPIQRVSYSVENARLGKVTDYEKLILEIWTNGAVSPEEALSRAARHL
                     KAHFSHLAPEGREEEDEEVEATGEGYLQESLAKPLEDLPLPARAINALKNHEIMVVAD
                     LVQKTDEDLESVKNLGEKSIDEIKAALAGLGLSLGMRIDPNLLGTLGRGSAK"
     gene            10246..10743
                     /gene="rplQ"
                     /locus_tag="VNU03_00395"
     CDS             10246..10743
                     /gene="rplQ"
                     /locus_tag="VNU03_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWP72931.1"
                     /translation="MRHGKAGFKLGRVTAHRWALFRNLLVALFRHERIMTTEAKAKAV
                     RGLADHMVTLAKREDLHARRQVLSMVPDAVVVKKLFDTIAARFSERHGGYTRIMKAGL
                     RAGDRAQMVVLELVDRPEAPKEKGKKDAKAEKAPPKEDKKAGKGEAGASAAKGRKKKA
                     AAAAG"
     gene            complement(10883..11557)
                     /locus_tag="VNU03_00400"
     CDS             complement(10883..11557)
                     /locus_tag="VNU03_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWP72932.1"
                     /translation="MAGKMVEFPSNGHTAKGYLATPAAGKGPGVIVIQEWWGLVGHIK
                     SVCDRFATAGYAALAPDLYHGQTASEPDAAGKLFMALNIERAEKDLAGAAKYLAPHSS
                     TAKLGAVGFCMGGQLALFAGCTNGSVGAVVDFYGIHPNVKPDYARLSGPVLGLFAEKD
                     AFVTPQAAREVAAAIKKAGKPVEVHIYPGVDHGFFNDERPDVYNKPAAEDAWRRTLDL
                     FKKNLK"
     gene            complement(11727..>11934)
                     /locus_tag="VNU03_00405"
     CDS             complement(11727..>11934)
                     /locus_tag="VNU03_00405"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HWP72933.1"
                     /translation="VNEALDWDLPKGDFETMAGLVLATLHRIPLVGEVFRVRRFTITV
                     LEADKRRVLAVRIVPDKKPTTPEA"
BASE COUNT         2081 a         3805 c         4351 g         1697 t
ORIGIN      
        1 ggaaggcttc cgtctggccg cgcagaagat cgggatcaag accaagttcg tgacccgtac
       61 gcgggcgctc tgaggcgacc atgaaggcgg ccaagtggcg tgagatgtcc gacgaggaag
      121 tgcagcagaa agcccaggag ctcggcgagg agctcttcaa cctgaggttc cagctctcga
      181 tgggggtggc caagaacccg gcccgggtgg cgatcgcccg gcgggatctc gcgcgggccc
      241 acaccattct gagagagcgg gcgctgcgca tcccgcggac ggcgtagagg cgggggagga
      301 caggacagtg ggcgtcaaga agacgcggga gggcgtggtg gtcagcgaca agatgaccaa
      361 gacgcgcgtg gtggtcatcg agcgcgtgtt ccgtcatccg cagtacgagc gcgtgatcac
      421 gcggtcgaag cgcctgaagg cgcacgacga gaagaacgcg agcaaggtcg gggaccgggt
      481 gctgatcgag gagacgcgac cgctgtcgaa ggaaaagcgg tggcgcatcc tccaggtgct
      541 ggcgagcgcg tcatgatcca gcctcgctcc atgctggacg tggccgacaa ctcgggggcc
      601 aagaaggtcc agtgcatccg ggtcatggga ggcgccaaca agcgctacgc ctccctgggc
      661 gacgtggtgg tggtggcggt caaggaggcg gtgccggacg ggaccgtgaa gaagggcgag
      721 gtcgcccggg cggtcgtggt gcgcaccgtg aaggaagtgg gacgccgtga cgggtcctac
      781 atccgcttcg accgcaacgc ggtggtcctc ctgaaggcgg acgacaaccc ggtgggcacc
      841 cgtatcttcg gtccggtggc gcgcgagctg cgggacaagc agttcaccaa gatcatctcg
      901 ctcgctcccg aagtcatcta gaggacgctc aatggcgatc gcgcacgtgc ggaagggcga
      961 cacggtgatg gtggtggcgg gcaaggagcg cggcaagaag ggcaaggtgc tccgggtgat
     1021 tcccgagaag ggccgggtgg tggtggagcg cctgaacctg atcaagaagc atcagaagcc
     1081 cacccagaag atccggcagg gcggcatcat cgagcgcgag gggtcgatcc acctctccaa
     1141 cgtgatgctg atcgaccccg gcagcgacaa gccgacccgc gtcggaatga aggccctgtc
     1201 ggacggcaag aaggtccggg tggccagacg gtcgggcgag atgctcgacc gagcgtgagg
     1261 acgtgatggc agagccgaag aaagcggacg ggaagggacc gggagcgaag gcgggcggag
     1321 ccgcgcccgg caaggcgggc caggcgggcc ggcccaaggg cggcggcaag gccgcgccgg
     1381 cgggagagcc acaggcgccg gccaagcggg cgagcgtcag cgggagcacg ccgccgcgca
     1441 tgcgcgagcg gttccggtcg gcggtggtgc ccgcgatgat gaaggagcgg ggctacggca
     1501 atccgttcca ggtgccgcgg ctcgagaagg tcgtgatcaa catgggggtc ggcgagggca
     1561 aggagaacgc gaaggtgctc gacttcgcca ccgcggacct gcaggcgatc gcgggccaga
     1621 agccggtgat cacccgggcc aagaagtcga tcgccaactt caagctgcgc gagaacgtgc
     1681 cgatcggctg caaggtgacc ctgcgcgggc cgcggatgta cgagttcctg gaccggctgg
     1741 tcaacgtggc gctgcctcgg gtccgggact tcaagggggt gccgcccaag ggcttcgacg
     1801 gccggggcaa ctacaacatg gggctcaagg agcagatcat ctttcccgag atcgtgtacg
     1861 acaaggtgga caaggtgcgc gggatggaca tcacgatggt gacgaccgcc cgaacggatg
     1921 aggaagcgaa ggcgctcctc acccacctgg gcgtgccttt cagggaggcg tagatggcca
     1981 agactgcgct catcatgaag cagcagcgcc cccccaagtt caagtcgcgc gcgtacaccc
     2041 gctgcaagat ctgtggccgc ccgcgcggct acctgcgcaa gttccaggcg tgccggctct
     2101 gcttccgcga gctcgcgctc aagggcgagg tgcccggcgt gatcaaggcg agctggtagc
     2161 cccatgatga gcgatccgat cgccgacttc ctgacccgca tccgcaacgc gagccgggcc
     2221 gagcacgaga aggtggacat cccgtcgtcg aagctcaagc tccgcatggc cgagatcctc
     2281 aaggacgagg ggttcatcaa gaacttccgg gtcatcgagg acaacaagca gggcgtgctg
     2341 cgggtctatc tgaagtacgc gggtactgag aagatcatct ccgggatcgt gcgcgtctcg
     2401 cgcccgggcc ggcggatcta cgtgaccaag gacaagatcc cgaccatcct gggcggcatg
     2461 ggggtcgcga tcctgtccac ctcggccggc gtgatgaccg accgccaggt gaagaagcag
     2521 cacctgggcg gcgaagtgct ggcctacgtc tggtaggagt ccctagccat gtcgcgaatc
     2581 ggcaagaagc ccatctcggt cccccaggga gtgaaggtcg ccctggacgg cgccatcgtc
     2641 cgcgcggagg ggcccaaggg caagctgagc caggccatcc cgtccggggt gtcggtgacg
     2701 atggactcga acgtgctgac ggtggcgcgc agctccgacc accgcaacgt gcgcgcgctc
     2761 cacggcctga cccggtcgct cgtggccaac atgatccacg gggtgaagga cggcttcgag
     2821 cggaagctcg agatcgtcgg catcggctac cggtgccagc tccagggcaa ggccctgcag
     2881 ctcgccctcg ggtactcgca cccggtcatc ttcccgcttc cggacgggat ccaggccgag
     2941 gtcgagcggc aggtgtcgat cacgctgcgg ggggcggaca aggcgctgct cggacagacc
     3001 gcggccaagc tgcgggcgct ccggaagccc gacccctaca agggcaaggg catcaagtac
     3061 tccgacgaat acattcgccg gaaggtgggc aagaaggcgg gggccaagta atgctgaccg
     3121 gcaagaaggt cgggggacgg aagatccgcc acctgcgcat ccgcacccgg gtgatcggca
     3181 ccgcggagcg cccgcggctg gcggtgttcc ggagcctgaa ccacatctac gcgcaggtga
     3241 tcgacgacac cagcgggcgc acgctcgccg cggtggacag ccgggccccg gacttccggc
     3301 agaaggtgaa ggcgggcggc aacgtggccg cggccaagat cgtgggggag ctgctggccg
     3361 agcgggcgaa ggcgcgcggc atcagccagg tcgtgttcga ccggggcggc tatcagtacc
     3421 acggacgggt gaaggccctc gccgacgcgg cacgcgccgc cggcctggga ttctagagag
     3481 gagcatcgag tggctgaggc gcggattgat gccaatgcgc tggacctgac cgaccgggtg
     3541 gtcgccatca accgggtggc gaaggtcgtc aagggcggcc gtcgattctc cttcaccgcg
     3601 ctggtggtgg tgggcgacgg gcaggggcac gtgggcgtgg ggctcggcaa ggcccgcgag
     3661 gtgcccgagg ccattcgcaa gtcggtggag cacgccaaga aggacctggt gaaggtgccg
     3721 ctcaaggaca ccacgatccc gtgcgagatc accggccact tcggcgccgc gcgggtgtat
     3781 ctcaagccgg cggcgcaggg caccggggtc atcgcgggcg gcgcggtgcg gccggtgctg
     3841 gaggcggcgg gggtgcagga catcctgacc aagaccctcg gcaccaacaa cccgcacaac
     3901 gtgctgaagg cgacgatgga cgccttccgc cggctcaagc ggctgcagtc cacgtacgcg
     3961 gcgcggcgcc ggtcgctcga cggcgacgcg caggagacgg ccggtgccaa agaagcttaa
     4021 ggtcacgctg gtgcggagca tcatcgggat gtcgccgacg caggaggcga cggtcaaggc
     4081 cctcggcctg cgccggatcc ggcaccaggt ggagcacgag gacaccccga cgatccgggg
     4141 catgatcgcc accgttcctt tcgcggtcga ggtacaagag tcatgaggct cgaggatctg
     4201 aggccggcgc cgggctcgac gaagaaccgc aagcggctcg gccgtgggcg ggggtcgggg
     4261 cagggcaaga cgtccggcaa gggccacaag ggtctgaacg cccggtcggg cggcggcgcc
     4321 cggcccgggt tcgagggcgg ccagatgccg ctctaccggc ggctgcccaa gcgcggcttc
     4381 ctgccctacg ggggcaagac cgagttcgcg atcgtgaacg tgggcgacct gtccgaccgc
     4441 ttcgcggcgg gcagcgtggt cgatcccgac gcgctggcgg ggagccggct catccacaag
     4501 tcggggcgcg cctcggtcaa ggtgctgggc gacggagacg tggcccacgc cctcacggtg
     4561 cgcgcgcaca aggtgagcga gggggcgaag cagaagatcg aggccgcggg cgggcgcgtc
     4621 gagatcctgg ccgcgcgcat cgcgagggcc gccaagtgat cgaaggcctg cagagcttcc
     4681 agaacatctt caaggttccg gagctcaagc gccgcgtgct catgaccctg gtgctgctcg
     4741 cggcgtaccg gctgggcgcc cacgtgccga ctcccggcat cgacgcggcc gcgctcgccg
     4801 agttcttcaa ccaggtgcag ggcacgctgc tcggcatggt cgatctcttc tcgggcggca
     4861 acctgcggcg gctgaccgtg ttcgcgctcg ggatcatgcc gtacatctcg gcctcgatca
     4921 tcctccagct gctcacggtg gtgatcccgg ccctggagcg gctggccaag gaaggcgagg
     4981 cgggcaagaa gaagatcacc cagtacaccc gctacggcac catcgcgctc tctctcgtgc
     5041 agtccttcgg catcgcggtg ggcctcgaga gcatgcggag ctcgaccggg gcggtgctcg
     5101 tccccgatcc gggctggggc ttccggctcc tgaccatgct caccctgacc accggaaccg
     5161 ccctgatcat gtggctgggc gagcagatct cggagaaggg catcggcaac ggcatctccc
     5221 tgatcatctt cgcggggatc gtggtgcgcc tgccctcggc gatcgtgtcc tcctacaccc
     5281 tgatcaccac cggcgagctc cagctcttcg tcttcctggg cttgatcatc atgatggtgg
     5341 cggtgaccgc ggcggtggtc gtcatgcagg agggccagcg caagatcccg gtccagtacg
     5401 ccaagcgggt ggtcgggcgg cggatgtacg gggggcaatc gacccacatc ccgctccgca
     5461 tcaacaccgc gggcgtgatc ccggtcatct tcgcctcgtc gctgatcctg ttcccggcga
     5521 ccctcacgcg cttcatccat cacccgtgga tgcagtacgt ctcggaggcg ctctcccccg
     5581 ggcacgtgac ctacaccgcg ctctactgcg cgctgatcat cttcttcgcc tacttctaca
     5641 ccgccatcgt gttcaacccg gttgacctgg ctgataacat gaagaagtac ggcggcttca
     5701 tcccgggcgt ccggcccggc aagaagaccg ccgagtacat cgaccgggtc ctgacgcgga
     5761 tcacgctgcc cggggccatc ttcctggcca tgatctcggt gcttcccgac ttcctgatcc
     5821 ggtggttcaa caccccgttc tacttcggcg ggaccagcct cctgatcgtg gtcggggtcg
     5881 cgctcgatac cgtgcgccag atggagtccc atctcctgat gcggaactac gaaggcttcc
     5941 tgcgcaagaa ggccaagggc gcatgagccg ggtcatcttc ctcggtgccc cgggcgccgg
     6001 caagggcacc caggctcggc gcctggccgg cgagtcgggc gtgccccagg tggccaccgg
     6061 cgacatgctg cgcgaggcgg tggccgaggg cacgccgctc ggtcgccagg ccaagcgcta
     6121 catggactcg ggggctctcg tgccggacga cgtggtgatc ggcctggtcg acgagcgcct
     6181 ggcccggccc gacgcgggcg gcggctacgt cctggacggg tttccccgca cggtggccca
     6241 ggcggaggcc ctcgacgcgc tcctgggccg gaggcgacag gagctggacc gggtgatctt
     6301 cttcgacgtg tcgcgcgacg agctgatccg ccggctcacc gggcgccgga tctgccggaa
     6361 gtgcgggacc gccttccacc tggtctcggc gcccccgaag gcggcgggcc ggtgcgatca
     6421 gtgcggcggc gagctgtacc agcgcgagga cgacgcggag gccacggtgg cccgccgcct
     6481 cgacgtgtac cagacccaga ccgcgcccct gctcgactac taccgccagc gcggcctgct
     6541 ggcgcgggtg gccggagagg gcccggtcga ggaagtggcc gaccgcatcc gcaaggcggt
     6601 caaggagccg gtgtcgcggt gatcctgctc aagtcggcac gcgagctcgc gcacatgcgg
     6661 acggcgggac gggtcctcgc cgaggtgaag gaccggcttc gctcactggt gcgccccggc
     6721 atctccacca aggagatcga cacggacgtg gaggccttca tcgtctcccg cggggcgacc
     6781 tccgccttca agggctaccg gggctttccc gccaccgtgt gcatttcgat caacgaggaa
     6841 gtggtccacg ggatcccctc tcccaagcgg aagctgcggg agggcgacat cgtggggctc
     6901 gatctcggct gcatcatcga gggctactac gcggactgcg cgatcaccct gccggtgggc
     6961 gcggtgccgc cgcgggtgca ggagctgctc gacgtcaccc gcgagagcct ggacaaggcg
     7021 atcgtgcagt gccgggccgg caaccgcctg ggcgatgtgt cgcacgcggt ccaggagcac
     7081 tgcgagagcc atggcttcgg ggtggtgcgc gccttcgtgg gccacggcat cgggcagagc
     7141 ctccacgagg agccgcagat cccgaacttc ggcgaggcgg gacggggtcc ggtcatcaag
     7201 gcgggcatgg tgctggccat cgagccgatg gtgacgatgg gctcgtggga ggtgcgcgtg
     7261 ctcgaggacc ggtggaccgc ggtgaccgtc gacgggagct gggccgccca cttcgaggac
     7321 acgatcgcga tcaccgagcg gggccccgag gtcctgaccc ggctggatca gggcgcctag
     7381 gacacccgcc atggccaaag aggacgcgat cgaggtggag ggcacggtgg tcgagccgct
     7441 gcccaatgcg atgttccggg tcgagctgga gaacggccac aaggtgctgg cccacgtgtc
     7501 gggcaagatg cggatgaact tcatccgcat cctgccgggc gacaaggtca aggtggagct
     7561 gtcgccctac gacctcacgc ggggccggat cacatatcgg ttcaagtaag ccgagcggat
     7621 agataaaggg gcaagggaat gaaggtcaga ccgtcgatca aggtccggtg cgagaactgc
     7681 aagatcgtgc gccgggaagg catcgtgcgg atcatctgca agaatccgcg ccacaagcag
     7741 cgccagggat aggagcgtac atggcacgta tcgccggagt cgatttgccg cgggacaagc
     7801 ggggtcagat cgcggtccag tacatctacg ggatcggcat cccgtcggcc aagaagatct
     7861 tcgccgacgc gcgggtcgat cccaacaagc gggtgaagac ctggagcgac gacgagaccg
     7921 gccgcgtccg cgacatcatc gagcgcgagt acaaggtcga gggcgacctg cgccgcgagg
     7981 tgtcgatgag cgtgaagcgg ctgatggaca tcggctgcta ccggggcatc cggcaccgca
     8041 aggggttgcc ggtgcgcggg cagcggaccc acaccaacgc ccgcacccgc aagggcaaga
     8101 gcaagggcgt gatggtcaag aagaagccgg tggccgccgc tccttcggcc ccgccgaaga
     8161 agtaaggagc cgagtcgatg gccgacgccg cggaacagaa gccggacgag aaggccgccc
     8221 cccgcaaggg tgggcgcaag cgggagcgca aggaggtgcg cgtgggcatc gcccacgtgc
     8281 aggccacctt caacaacacc atcgtgacct tgaccgacaa gatgggcaac gtggtctcgt
     8341 gggcgagcgc gggcagcgtg ggcttcaagg gctcgcgcaa gagcaccccg ttcgcggcgc
     8401 agaccgcggc ggagaacgcg gcccgcaagg cgatggaagt ggggctcaag caggtggagg
     8461 tcttcgtgcg cggcccggga gccgggcgcg aggccgcgat ccgctccctg caggcggtgg
     8521 ggctcgagat caccgccatc cgtgacgtga ccccgattcc gcacaacggc tgccgtccgc
     8581 ccaagcggcg acgcgtgtag gaggagacgt aagtggcacg atatcgagcc gccgtctgcc
     8641 ggctgtgccg gcgtgaaggc atcaagctgt tcttgaaggg atcgcgctgc ttcaccgaga
     8701 agtgcgcgat cgagcggcgg aactatcccc cgggccagca cgggctgggt cgcaccaagc
     8761 cgacccagta cggcatccag ctgcgcgaga agcagaaggc caagcgcatc tacggggtgc
     8821 tcgaggcgca gttccggcgg tacttcgcgt gggcggaggc cgacaagggc atcaccggcg
     8881 agaacctgct caagtatctc gagcggcgcc tcgacaacgt ggtgttccgc ctcggcatgg
     8941 ccgcctcccg ccgggaggcc cggcagatga tcggccacgg ccacgtccag gtgaacgggc
     9001 ggaaggtgtc gatcccgtcc ttcatcgtga aggtgggcga ggtggtgcag ctccgatcga
     9061 cctccaagat gcaggagcgg gtggccgaca acctgaccgc gggccggtcg ccggttccgc
     9121 cgtggctcga cctggacgtg aacgagaaga aagggacggt gcgcgggctt ccgctccggg
     9181 aagacatcca gatcccggtg caggagcagc tcatcgtcga gctgttctcg aagtagggcc
     9241 aagcggccaa cagaaggggc atatggcgag gattccgttt cagaagccga agtcgatcca
     9301 gtgggagatc cgatccgatc gctacgggcg gctggtcgcg gagccgttcg agaagggcta
     9361 cgcgctcacg gtggggcatt ccctccgtcg cacgctcctg gccatcgtgc cgggctcggc
     9421 ggtggcctgg gtgcgcatcc cgggggtcaa ggatctcgac acccggattc cgggagtcca
     9481 ggaagacacg accgacgtcc tcctgaacgt caagaagctg gccgtgaacg tgccgtccgg
     9541 tgagccgacc gagacgcggc tggaggccaa ggggcccaag acggtgaccg gggccgacgt
     9601 gtcggaggcg accgggatcg aggtgctgaa cccggagatc cacatcgcca cgctgggagc
     9661 gggcggctcg ctctcgatgg acctgggcat cggcgtcggt cgtggttacg tggcggccga
     9721 tcggcatccc gcgggcacgg tgccggcggg ggcgatcccg ctggacgccg cgttctctcc
     9781 gatccagcgc gtgtcctaca gcgtggagaa cgcccggctc ggcaaggtca ccgactacga
     9841 gaagctgatc ctcgagatct ggaccaacgg cgcggtgtcc cccgaggagg cgctctcccg
     9901 ggcggcccgc cacctgaagg cccacttctc ccacctggcc cccgaggggc gcgaggagga
     9961 ggacgaggag gtcgaggcca ccggcgaggg ctacctgcag gagagcctgg ccaagccgct
    10021 ggaggacctg ccgctccccg cgcgcgccat caacgcgctg aagaaccacg agatcatggt
    10081 cgtggcggat ctggtccaga agaccgacga ggacctggag tcggtgaaga atctgggcga
    10141 gaagtcgatc gacgagatca aggccgcgct ggccggcctc gggctttccc tgggtatgcg
    10201 catcgacccg aacctgctgg gcacgctcgg gcgggggagc gcgaaatgag gcacggcaag
    10261 gcgggcttca agctcggacg cgtcaccgcg caccgctggg cgctcttccg gaacctgctg
    10321 gtcgcgctct tccggcacga gcggatcatg accaccgagg ccaaggcgaa ggcggtgcgg
    10381 ggcctcgccg accacatggt caccctggcc aagcgagagg atctgcatgc gcgccggcag
    10441 gtgctgtcga tggtgccgga cgcggtggtg gtgaagaagc tcttcgacac catcgcggcc
    10501 cgcttcagcg agcgccacgg cggctacacc cgcatcatga aggcgggcct gcgtgccggc
    10561 gaccgcgccc agatggtggt cctcgagttg gtggaccgtc cggaggcccc gaaggagaag
    10621 gggaagaagg acgccaaggc cgagaaggcg ccgcccaagg aagacaagaa ggccgggaag
    10681 ggcgaagccg gcgcgagcgc cgccaagggc cgcaagaaga aagcggcggc cgcggcgggc
    10741 tagcccgagg tcatccccgg gtgtccgcca gtcgggatgc ggaccggttg tccgctgact
    10801 gggggagtgc gggggccatt tcggggcccc cgcagttgaa gagcctgaag aactaaacgg
    10861 ttgtccgccc tcgagccccc ggttacttca ggttcttttt gaacaggtcc agcgtccgcc
    10921 gccaggcgtc ctcggcggcc ggcttgttgt agacgtcggg ccgctcgtcg ttgaagaagc
    10981 cgtggtccac ccccgggtag atgtggacct cgaccggctt gcccgccttc ttgatcgcgg
    11041 ccgccacctc gcgcgcggcc tgcggggtca cgaacgcgtc tttctccgcg aagaggccga
    11101 gcaccggacc gctcaggcgg gcgtagtccg gtttcacgtt cgggtggatg ccgtagaagt
    11161 ccaccaccgc gcccacgctt ccgttggtgc agcccgcgaa gagcgcgagc tggccgccca
    11221 tgcagaagcc caccgcgccg agcttggcgg tcgaggagtg cggggcgagg tacttggccg
    11281 cgccggccag gtccttctcg gcccgctcga tgttgagcgc catgaagagc ttgcccgccg
    11341 cgtccggctc gctcgcggtc tgcccgtgat agagatcggg cgcgagcgcc gcgtagccgg
    11401 cggtcgcgaa tcgatcgcac acgctcttga tgtgcccgac caggccccac cactcctgga
    11461 tcacgatcac gcccggtccc ttgccggcgg ccggcgtcgc cagatagccc ttcgcggtgt
    11521 gtccgttgct cgggaactcg accatcttgc ccgccatggg agtcatcctt tcttagtcgg
    11581 tgagttcaat cgtcacggtt ggcggttcgg cctcttcgcg cccttcggcg ctcatcgggg
    11641 cgtcgacgcc taccgtaccg caaaattgcc tgctgcggaa cgtccccggc cggcgccagg
    11701 tcacggccga ccatgtgtgc cccccgtcag gcttcggggg tggtgggttt cttgtcgggg
    11761 acgatgcgca ctgcgaggac gcgacgcttg tccgcctcga gcacggtgat ggtgaagcgg
    11821 cggacgcgga acacctcgcc cacgagcggg atacggtgca gggtggcaag gacgaggccg
    11881 gccatcgtct cgaagtcgcc cttgggcaga tcccaatcga gtgcctcgtt gacc
//
LOCUS       DATMCV010000011         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_931100, whole genome shotgun sequence.
ACCESSION   DATMCV010000011 DATMCV010000000
VERSION     DATMCV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931100"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="VNU03_00410"
     CDS             complement(<1..1608)
                     /locus_tag="VNU03_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP72934.1"
                     /translation="MDIVPSVCPHDCTSTCALEVERIDARTIGRVRGSHRNTYTRGVI
                     CEKVGRYAERVHHPDRLLHPLRRVGPKGSGRFERIGWDEALDTVAEAFTRQARSLGPE
                     TVWPYYYAGTMGLVQRDGINRLRHALGYSRYYSTICVTLSDTGWAAGVGAKRGVDLRE
                     IDEHSDLVVIWGGNPVNTQVNVMSHAMAAKRRGAPLVVVDPYRTDTAAKADLHLAPRP
                     GTDGALACAVMHVLFAEGLADWDYLRAYTDCPDELAAHVAARPPAWAAAITGLDAEQI
                     VDFARLYGRTKRSFIRCHHGFSRSRNGAVNMHAVSCLPAITGAWQYPGGGAVYGQTAL
                     YPLDRSLIEGLDLVDKSIRALDQSRLGQILTGDPAALLGGPPVTAMLVQNTNPAMVCP
                     ETHLVHRGLAREDLFLCVHEQFLTETAAFADIVLPATMMVEHDDFYTASGHTYFQVTK
                     QVIEPPGECRENHWVICQLAKRLGATHPGFQMSAWEIMDETLRKSGMWDAETNFRQGG
                     QDFAPPFETAHFLDGFPTPDRRFHFKPA"
     gene            1653..1964
                     /locus_tag="VNU03_00415"
     CDS             1653..1964
                     /locus_tag="VNU03_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72935.1"
                     /translation="MADERPKTAYELAMERLSQKDRSAGGTAAPLTDEQKTAIAEARQ
                     LHQSRVAEREILHHAALRKAGTHEEVDQLNEALRRDMERLATDRDRKIAQIRGEKPQT
                     A"
     gene            complement(1952..>2408)
                     /locus_tag="VNU03_00420"
     CDS             complement(1952..>2408)
                     /locus_tag="VNU03_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWP72936.1"
                     /translation="WLEFMDWAAPVVEEPVRHDDYEGYACIAAEAVTPIQTGENLVDT
                     FEMARAIALHSLDFVMPDVQRIGGVSGWLRAAALAHAHGIEMSSHLFPEFSVHLLGVT
                     PTCHWLEFMDWAAPVVEEPLAVKAGFARIPDRPGAGIRWNEEAVTRYAV"
BASE COUNT          356 a          826 c          886 g          340 t
ORIGIN      
        1 ggccggcttg aagtggaagc gccggtcggg ggtggggaag ccgtcgagga agtgcgcggt
       61 ctcgaacggc ggggcgaagt cctggccgcc ttgccggaag ttcgtctccg cgtcccacat
      121 gcccgacttc cgcagggtct cgtccatgat ctcccaggcg ctcatctgga agccggggtg
      181 ggtggccccg aggcgcttgg cgagctggca gatcacccag tggttctcgc ggcactcgcc
      241 gggcggctcg atcacctgct tggtgacctg gaagtaggtg tgcccgctcg cggtgtagaa
      301 gtcgtcgtgc tcgaccatca tggtggcggg cagcacgatg tcggcgaagg cggcggtctc
      361 ggtcaggaac tgctcgtgca cgcagaggaa gaggtcctcg cgggccaggc cccggtgcac
      421 caggtgggtc tccgggcaca ccatcgccgg attcgtgttc tgcaccagca tcgcggtcac
      481 cggcgggccg cccagcaggg ctgcggggtc gccggtgagg atctggccca gacgcgattg
      541 gtccagcgcg cggatggact tgtcgaccag gtcgaggccc tcgatcagcg agcggtcgag
      601 gggatagagc gcggtctggc cgtagaccgc gccgccgccg ggatactgcc aggccccggt
      661 gatcgcgggc aggcaggaca ccgcgtgcat gttcaccgcc ccgttccggg agcggctgaa
      721 gccgtggtgg cagcggatga agctcctctt cgtgcgcccg tagaggcgcg cgaagtccac
      781 gatctgctcc gcgtcgaggc cggtgatggc ggcggcccac gcgggcggac gggcggccac
      841 gtgggcggcc agctcgtccg ggcagtcggt gtacgcgcgc aggtagtccc agtcggccag
      901 gccctcggcg aagagcacgt gcatgaccgc gcaggccagc gcgccgtcgg tgccggggcg
      961 cggggccaga tgcaggtcgg ccttcgcggc ggtgtcggtg cgatacgggt cgaccaccac
     1021 gagcggggcg ccgcggcgct tggccgccat cgcgtggctc atcacgttga cctgggtgtt
     1081 gaccgggttg ccgccccaga tcaccaccag gtcggagtgc tcgtcgatct cgcgcaggtc
     1141 cacgccgcgc ttggctccca cccccgcggc ccagccggtg tcggagaggg tcacgcagat
     1201 cgtcgagtag tagcgcgagt agccgagcgc gtggcgtagg cggttgatgc cgtcgcgctg
     1261 gaccaggccc atggtgcccg cgtagtagta gggccacacg gtctccggac cgaggctccg
     1321 ggcctggcgg gtgaaggcct ccgccactgt gtcgagcgcc tcgtcccagc cgatgcgctc
     1381 gaagcggccc gagcccttcg gccccacgcg ccgcagcggg tgcagcaggc ggtccggatg
     1441 gtgcacgcgc tcggcgtagc ggcccacctt ctcgcagatg acgccgcggg tgtaggtgtt
     1501 acggtgcgag ccgcgcaccc ggccgatggt gcgcgcgtcg atccgctcga cctcgagggc
     1561 gcaggtgctg gtgcagtcgt gcgggcagac cgacgggacg atgtccatgg cctagagaat
     1621 accgcaggtg acgagtctgc tacactcccg ccatggccga cgagcgaccc aagaccgcgt
     1681 acgagctggc gatggagcgc ctctcccaga aggaccggtc ggcgggcggg acggcggctc
     1741 cgctcaccga cgagcagaag acggccatcg cggaggcccg ccagctccac cagtcacgcg
     1801 tcgcggagcg ggagatcctg caccacgccg ccctgcgcaa ggcgggcacc cacgaggagg
     1861 tcgaccagct caacgaggcc ctccgccgcg acatggagcg gctcgccacc gatcgcgacc
     1921 ggaagatcgc gcagatccgc ggcgagaagc ctcagaccgc gtagcgcgtc accgcctcct
     1981 cgttccaccg gatgccggcc cctgggcggt cgggaatccg ggcgaagccc gccttcaccg
     2041 cgagcggctc ctcgaccacc ggcgcggccc agtccatgaa ctcgagccag tggcaggtcg
     2101 gggtcacccc cagcagatgc acgctgaact ccgggaagag atggctcgac atctcgatgc
     2161 cgtgggcgtg cgcgagcgcc gccgcccgca gccagccgct caccccgccg atgcgctgca
     2221 cgtcgggcat cacgaagtcg agcgagtgga gcgcgatggc ccgcgccatc tcgaaggtgt
     2281 ccaccaggtt ctcgccggtc tggatcgggg tgaccgcttc ggccgcgatg catgcgtagc
     2341 cctcgtagtc gtcgtggcgc accggctcct cgaccaccgg cgcggcccag tccatgaact
     2401 cgagccag
//
LOCUS       DATMCV010000012         2329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_599253, whole genome shotgun sequence.
ACCESSION   DATMCV010000012 DATMCV010000000
VERSION     DATMCV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599253"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VNU03_00425"
     CDS             <1..901
                     /locus_tag="VNU03_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP72937.1"
                     /translation="STTLRLIAGLEAATAGRVAIGGVDVTHRPPAERRVSMVFQSYAL
                     FPHLSVAENIVFGLRVRRVSAAERRERLARVADLLGLTALLERRPSQLSGGQQQRVAL
                     GRALIAEAPVCLMDEPLSNLDAQLRLEMRREIRGLQQSLGITMVYVTHDQTEAMTMAD
                     QVILLREGRVEQDASPEALYARPSTAFAARFIGTPPMNVLALDDRRRLLGVRPEDIRV
                     LDAKEPGAVEALVRSIEYLGADSILMCAIGRQALAVRAPGRVALAPGAPVSLAWARDA
                     AHVFDAETGRRRDDVAADSPISR"
     gene            916..2205
                     /locus_tag="VNU03_00430"
     CDS             916..2205
                     /locus_tag="VNU03_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP72938.1"
                     /translation="MIRTLLAGLLLVALTGGASPVIAQAPVELSFYYPVAVGGPVTKI
                     IDGLAADFQKENPGIHVKPIYAGTYQETIVKVLTALKSGEPPQMSVLLSTDMFTLIDE
                     DAIVPFDDLATGAEDRSWLKSFFPAFMLNSQTGGKTWGVPFQRSTIVLYWNKEAFKEA
                     GLDPSKPPATWAEQVEYARKLTKRDGSGNVTQWGIEVPSSGFPYWLFQGFTTQNDVLL
                     MNQAGNETYYDKPAVVEALQYWMDLSGKHKVMPTGIIEWGTTPKDFFERKTAMMWTTT
                     GNLTNVKNNAKFEFGVAMLPAGKRRGSPTGGGNFHVFKKSTPAQQAASLKFIKWITSP
                     QRAAQWGIDTGYVAVRADAWETPAMKQYVAGFPAAAVARDQLQYAVAELSTHDNQRVT
                     KALNDGLQAALTGAKPADQAMKDAQREAERILRPYRR"
BASE COUNT          372 a          832 c          800 g          325 t
ORIGIN      
        1 gtccaccacg ctgcgcctca tcgcggggct cgaggcggcc accgcggggc gcgtcgccat
       61 cggcggtgtc gacgtcaccc accggccgcc cgccgagcgg cgcgtgtcca tggtcttcca
      121 gtcctacgcg ctcttcccgc acctctcggt cgcggagaac atcgtgttcg gcctccgggt
      181 gcgtcgcgtg agcgccgccg agcgcagaga gcggctcgcc cgcgtggccg acctgctggg
      241 gctgaccgcg ctgctggagc gccggccctc gcagctctcc ggcggtcagc agcagcgggt
      301 ggccctcggt cgcgcgctga tcgcggaggc gccggtgtgc ctgatggacg agccgctctc
      361 caacctggac gcccagctcc ggctcgagat gcgccgggag atccgcggcc tccagcagag
      421 cctcggcatc accatggtct acgtcaccca cgaccagacc gaggcgatga ccatggccga
      481 tcaggtcatc ctgctgcgcg agggccgcgt ggagcaggac gcgtcgccgg aggcgctcta
      541 cgcgcgcccg tcgactgcct tcgcggcgcg cttcatcggc accccgccca tgaacgtgct
      601 cgccctcgac gatcggcggc ggctgctggg ggtgcggccg gaggacatcc gcgtcctgga
      661 cgcgaaggag cccggcgcgg tggaggccct ggtgcggagc atcgagtatc tcggcgccga
      721 ctccatcctg atgtgcgcga tcggccgtca ggcgctcgcg gtgcgcgccc ccggccgcgt
      781 cgccctcgcc ccgggcgcgc ccgtgtctct cgcgtgggcg cgggacgcgg cccacgtgtt
      841 cgacgccgag accggccgcc gtcgcgacga cgtggcggcc gactcaccca tctctcgatg
      901 acgaggagac cattcatgat caggacactg ctcgcaggcc tgctgctcgt cgccctcacc
      961 ggcggcgcct cgccggtgat cgcccaggcc ccggtggagc tctccttcta ttacccggtg
     1021 gcggtcggag gcccggtgac gaagatcatc gacggcctcg ccgcggactt ccagaaggag
     1081 aaccccggca tccacgtcaa gccgatctac gccggcacct accaggagac gatcgtcaag
     1141 gtgctcaccg cgctcaagag cggcgagccg ccccagatgt cggtgctgct ctcgaccgac
     1201 atgttcaccc tgatcgacga ggacgcgatc gtccccttcg acgacctcgc caccggcgcg
     1261 gaggaccgga gctggctcaa gagcttcttc cccgccttca tgctcaacag ccagaccggg
     1321 ggcaagacct ggggcgtgcc gttccagcgc tccaccatcg tgctgtactg gaacaaggag
     1381 gccttcaaag aggccggcct cgacccgagc aagccgcccg ccacctgggc cgagcaggtc
     1441 gagtacgcgc gcaagctcac caagcgcgac ggcagcggca acgtcaccca gtgggggatc
     1501 gaggtgccgt cctccggctt cccgtactgg ctcttccagg gcttcaccac ccagaacgac
     1561 gtgctgctga tgaaccaggc gggcaacgag acctactacg acaagccggc ggtggtggag
     1621 gcgctccagt actggatgga cctgagcgga aagcacaagg tcatgcccac cggaatcatc
     1681 gaatggggca cgaccccgaa ggacttcttc gagcgcaaga ccgccatgat gtggaccacc
     1741 accggcaacc tgaccaacgt gaagaacaac gccaagttcg agttcggggt ggcgatgctg
     1801 ccggcgggca agcggcgcgg ctcgccgacc ggcggcggca acttccacgt cttcaagaag
     1861 agcacgccgg cccagcaggc cgcctcgctc aagttcatca agtggatcac gtcgccacag
     1921 cgggcggcgc agtggggcat cgacaccggc tacgtggcgg tgcgcgcgga cgcgtgggag
     1981 acgccggcca tgaagcagta cgtcgccggc ttccccgccg ccgcggtggc gcgcgatcag
     2041 ctccagtacg cggtcgcgga gctgtccacg cacgacaacc agcgggtgac caaggcgctc
     2101 aacgatgggc tgcaggcggc gctgaccggg gcgaagcctg ccgaccaggc catgaaggac
     2161 gcccaacggg aggccgaacg gatcctgcgc ccataccgcc ggtgatgccc ggcctcgcgg
     2221 gtggtcgggt gggggtgggg tggccggcgg ggcgggcctc ccgggaaccg tgtcttctgc
     2281 gcgtggtcga agtcgcagac caaaatctcg tggctcgcca tgagtgcag
//
LOCUS       DATMCV010000013         2156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_599356, whole genome shotgun sequence.
ACCESSION   DATMCV010000013 DATMCV010000000
VERSION     DATMCV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599356"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /gene="pgi"
                     /locus_tag="VNU03_00435"
     CDS             complement(<1..1467)
                     /gene="pgi"
                     /locus_tag="VNU03_00435"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWP72939.1"
                     /translation="MAGEREAWAALRRHHEQIRDVHLRTFFAEGRNRGERLVLEGAGL
                     YLDYSKNRVTDETLALLLALAEAAGLRARIDAMFSGQKVNITEGRAVLHVALRAPRDR
                     RILVDGVDVVPGVHAVLDRMADFSTRVRSGAWTGFTGRPIRSVVNIGIGGSDLGPVMA
                     YEALRHYSDRRLTLRFVSNVDGADFVESTRDLDPAETLFVVSSKTFTTQETLTNAHAA
                     REWSLRTLGDERAVARHFVAVSTNGAEVTKFGIDTANMFEFWDWVGGRYSFDSAIGLS
                     LMIAIGPEHFRAMLGGMHEMDEHFRTAPFDRNLPVLLGLLGVWYVNCFGAETHAILPY
                     SQYLARLPFYLQQLDMESNGKSVDWDGRRVDYQTGPVIWGTPGTNGQHAYYQLIHQGT
                     RLIPADFIGFSRSLTPLGRHQDLLTANLIAQTEALAFGKTAAEVAAEGVPAAQVPHRT
                     FEGNRPSNTILAEELTPAALGRLVALYEHKVFVQGTIWR"
     gene            complement(1457..>2156)
                     /locus_tag="VNU03_00440"
     CDS             complement(1457..>2156)
                     /locus_tag="VNU03_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HWP72940.1"
                     /translation="LADQAGLRRFGFHGLSYEYVMEHLGPAGRGRVIIAHLGNGASMA
                     AVRAGRPVDTTMGLTPAGGFMMGTRTGDLDPGVLVYLAREKGYDANGLDRLVNKESGL
                     LGVSGVSADMKTLLERRERDEGAALAVAMFVYQARKQVGALAAALGGLDTLVFTGGIG
                     ERAAPVRAEICDGLGHLGVHLDPARNAAHGSPTSVPGRGCQVRIVATREDLMIARHTR
                     GVLFPDGERRDRGR"
BASE COUNT          301 a          793 c          746 g          316 t
ORIGIN      
        1 gcgccagatg gtcccctgca cgaacacctt gtgctcgtag agcgcgacca gccgcccgag
       61 cgcggcgggg gtcagctcct cggccaggat ggtgttgctc ggccggttgc cctcgaaggt
      121 tcggtgcggg acctgggccg cgggcactcc ttccgccgcg acctccgccg cggtcttgcc
      181 gaacgcgagc gcctcggtct gggcgatgag gttggcggtc aggagatctt ggtgacgccc
      241 gagcggggtg aggctgcgcg agaagccgat gaagtcggcc gggatgagcc gcgtgccctg
      301 gtggatgagc tggtagtagg cgtgctggcc gttggtcccc ggcgtgcccc agatcaccgg
      361 cccggtctgg tagtcgaccc gacgcccgtc ccagtccacc gacttgccgt tcgactccat
      421 gtcgagctgc tgcaggtaga acggcagccg ggccaggtac tggctgtagg gcaggatggc
      481 gtgggtctcc gccccgaagc agttcacgta ccagaccccg agcagtccga gcagcaccgg
      541 caggttccga tcgaacgggg cggtgcggaa gtgctcgtcc atctcgtgca tcccgcccag
      601 catcgcgcgg aagtgctcgg ggccgatcgc gatcatgagg gagagcccga tcgcggagtc
      661 gaaggagtag cggccgccca cccagtccca gaactcgaac atgttcgcgg tatcgatgcc
      721 gaacttggtg acctccgccc cgttggtcga caccgccacg aagtgccgcg ccaccgcgcg
      781 ctcgtcgccg agcgtgcgca ggctccattc ccgggccgcg tgcgcgttgg tgagcgtctc
      841 ctgggtggtg aaggtcttcg acgacacgac gaagagggtc tcggccgggt cgaggtcccg
      901 cgtcgactcg acgaagtcgg cgccgtccac gttggagacg aagcgtagcg tgaggcgccg
      961 gtcgctgtag tggcgcagcg cctcgtaggc catgaccggg cccaggtcgg agcccccgat
     1021 gccgatgttg accacgctgc ggatgggccg gccggtgaag ccggtccagg cgccgctccg
     1081 cacccgcgtc gagaagtcgg ccatccggtc cagcaccgcg tgcacgccgg gcaccacgtc
     1141 cacgccgtcg accaggatgc ggcgatcccg cggggcccgc agcgccacgt ggagcaccgc
     1201 acggccctcg gtgatgttga ccttctgccc cgagaacatc gcgtcgatcc gcgcgcgcag
     1261 gcccgccgcc tcggccaggg cgagcagcag cgccagggtc tcgtcggtga cgcgattctt
     1321 cgagtagtcg agatagagcc cggccccttc gaggacgagc cgctcgccgc ggttgcggcc
     1381 ctccgcgaag aacgtccgca ggtggacgtc ccggatctgc tcgtggtgcc gccgcagcgc
     1441 cgcccaggct tccctctcac cggccacgat ctctcctctc gccgtcgggg aacaggactc
     1501 cccgggtgtg ccgcgcgatc atcagatcct cccgggtcgc cacgatccgc acctggcagc
     1561 cgcggcccgg cacgctcgtc ggactgccgt gcgccgcgtt gcgcgccgga tcgaggtgga
     1621 cccccaggtg gccgagcccg tcgcagatct ccgcgcgcac cggcgcggcc cgctctccga
     1681 tgccgcccgt gaagaccagc gtgtcgaggc cgcccagcgc cgccgcgagg gcccccacct
     1741 gcttgcgggc ctggtacacg aacatcgcga ccgcgagcgc cgccccttcg tcgcgctcgc
     1801 gccgctcgag cagcgtcttc atgtccgcgc tcacgcccga caccccgagc agccccgact
     1861 ccttgttcac caggcggtcg aggccgttcg catcgtagcc cttctcccgc gccaggtaga
     1921 cgagcacccc cgggtcgagg tcgccggtgc gcgtgcccat catgaacccg cccgccgggg
     1981 tgagccccat ggtcgtgtcc accggacgcc cggcgcgcac cgcggccatg ctcgccccgt
     2041 ttcccaggtg cgcgatgatc acgcggcccc ggccggccgg accgaggtgc tccatcacgt
     2101 actcgtagga tagcccgtgg aagccgaagc gccggaggcc ggcctggtcc gccagc
//
LOCUS       DATMCV010000014         3741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_599410, whole genome shotgun sequence.
ACCESSION   DATMCV010000014 DATMCV010000000
VERSION     DATMCV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599410"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VNU03_00445"
     CDS             complement(<1..866)
                     /locus_tag="VNU03_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWP72941.1"
                     /translation="MPRRLALVSILCSIAVVGTASVAPAQTAPQYVWKAGTVTAGGNP
                     LNDTMEHFAKQLTEKSRGRIKVDVHIANALAKGEAAHLEGVQLGTIDVVAIGSAPVGG
                     MFEPLYQSLDLPFLWSTREQVWKVMDGPLGQELLKKMEAKNLRAFCFGGGWGFRNMMS
                     NKRPVVTPDDMKGQTIRVQESPTYVAMMKAMGANPVPMPYVEVYLAMKQGTIDGMELP
                     SFTFTSDKFQEVTKYYSLTRHSYPPIAWFMNLKKYQALPADVQRLVDDSMKDACQLHR
                     RLEVEKEKSDFDV"
     gene            1056..1595
                     /locus_tag="VNU03_00450"
     CDS             1056..1595
                     /locus_tag="VNU03_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72942.1"
                     /translation="MTEPTLDGLHGRLERLEREVTWWRRAGIAALACAGLLGAVAATV
                     STNPDEVKTRRLVITDGDGRARAVFTVDDSDRTRLSLTDRDNGTTADLTVVPGQSAAL
                     SLARAGAQAQLATTGDNGQLSVGTRGQRGWLVAAPSGSSLGLGNESGKPQISLATSPT
                     LAPGLLLSDRDGNVVWKAP"
     gene            1725..3479
                     /locus_tag="VNU03_00455"
     CDS             1725..3479
                     /locus_tag="VNU03_00455"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWP72943.1"
                     /translation="MPQGPINREVAFAGQQVRRKVIFALTLCSVLPLLLLTYAFSTPV
                     RELLGPLAGLADAISIPALLVFTAILMAGGAFVVWDIATALSRAAQISNEDNPALSAE
                     PGRKDEIGTLMSSFARMLATIQQQSQEINEFPRRLDQLIREAFRDSLTSLPNRALFMD
                     RLSHALTRAERAGTNLAVLFLDLDRFKILNETLGHEVGDRLLVEVGHRIAACLRPEDT
                     VARLGGDEFGLLLEDTADLAGATAVAERVSTEIQRPFVVEGRDVLISASIGIALTAGG
                     SMQPEEVLHNADLAMYQAKAEGRGRYELYQPGLSVSTRERLDLQSDLRTAGARQELTL
                     RYQPVVTLATLRPVEVEALIRWDHRRRGALLPADFIALSEETGLMVPMGQWVLREACR
                     QTRAWQGDGSATPPLVVGVNLSASQFERDALPEEIGTILRETGLQPSRLQLEISEAVL
                     MRDDPRMLHRLEALKGIGVRLAIDDFGTGYASLSYLKRLPVDCLKIDRSLVKGVGSDT
                     EDTAIVRAVVALARSLGIVVTAEGVETAEQLFQLRALGCEQGQGYYFARPVSADRLPE
                     LLSSLDADDRHGSPSCLG"
     gene            3467..>3741
                     /locus_tag="VNU03_00460"
     CDS             3467..>3741
                     /locus_tag="VNU03_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72944.1"
                     /translation="MSWVALRALLRTILIRLIGIAIFTGALGLGLAWLLDVAVSEPEW
                     TETAAIAQRAVDRSGLPELLAGQSARSPKRWADESARFVRELPGVVQ"
BASE COUNT          528 a         1363 c         1269 g          581 t
ORIGIN      
        1 acgtcgaagt cgctcttctc cttctcgacc tcgagccgcc ggtggagctg gcaggcgtcc
       61 ttcatcgagt cgtccaccag cctctggacg tcggcgggaa gggcctggta cttcttgagg
      121 ttcatgaacc aggcgatcgg cggatacgag tgccgggtca gcgagtagta cttggtcact
      181 tcctggaact tgtccgaggt gaacgtgaac gagggcagct ccatcccgtc gatcgtcccc
      241 tgcttcatcg cgaggtacac ctcgacgtag ggcatcggca ccgggttcgc gcccatcgcc
      301 ttcatcatgg ccacgtaggt cggcgactcc tgcacgcgaa tggtctgccc cttcatgtcg
      361 tcgggggtga ccaccggacg cttgttggac atcatgttgc ggaagcccca gccgccgccg
      421 aaacagaagg ccctcaggtt cttggcctcc atcttcttga gcagctcctg cccgagcgga
      481 ccgtccatga ccttccacac ctgctcgcgc gtcgaccaga ggaagggaag gtcgagggac
      541 tggtagagcg gctcgaacat gccgcccacc ggcgcgctgc cgatcgcgac cacgtcgatg
      601 gtgccgagct gcacgccctc gagatgtgcg gcctctccct tggcgagcgc gttggcgatg
      661 tggacgtcga ccttgatccg gccccggctc ttctcggtga gctgcttggc gaagtgctcc
      721 atcgtgtcgt tcagcgggtt gccgcccgcg gtcaccgtgc ccgccttcca cacgtactgg
      781 ggcgcggtct gggccggcgc gacggaggcc gtgcccacga ccgcgatcga gcacaggatc
      841 gacacgagag ccaggcgacg gggcatggtc agttctcctc gaaatcgagt gggcgcggaa
      901 cggggccgga tcgcgtcatc ggaagccgcc cctgtgtagc acgcgggctc ggggagggtc
      961 aaggcgggga gcctctggac ttcggccccg gcccggggca ggctggggtc gcggccgctc
     1021 ccgcggcccc actcgcccca ccacggagga gcacgatgac ggagccgact ctcgatgggc
     1081 tgcacgggcg cctggagcgc ctcgagcgcg aggtcacctg gtggcgccgc gcgggcatcg
     1141 cggcgctggc ctgcgcggga ttgctcggcg cggtggcggc caccgtcagc acgaacccgg
     1201 acgaggtgaa gacgcgccgg ctggtgatca cggacggcga cgggcgcgcc cgcgccgtct
     1261 tcacggtgga cgacagcgat cgcacgcgcc tgtcgctcac cgatcgcgac aacggcacca
     1321 ccgccgatct caccgtcgtc ccgggccagt ccgccgcgct ctccctcgcc cgcgcggggg
     1381 cgcaggcgca gctcgccacc accggcgaca acggccagct ctcggtcggc acccgcggcc
     1441 agcgcgggtg gctggtcgcg gccccgagcg ggtcgagcct cgggctcggc aacgaatcgg
     1501 gcaagccgca gatctcgctc gccaccagcc cgacgctcgc ccccggcctt ctgctctcgg
     1561 accgcgacgg caacgtggtg tggaaggctc cgtagacgaa aatcgtcgcc tttctcgaca
     1621 atttgtaagc gcgcgggcgc agtcgggccc gtgttatcag ccggttagac cgcccactcc
     1681 gtggcactcc tcttgctcga tccgtcaccc cttggagcct cttcatgccc cagggaccga
     1741 tcaatcgtga ggtggcgttc gccggccagc aggtccggcg caaggtcatc ttcgccctca
     1801 ccctgtgttc ggtcctcccg ctgctgctcc tcacgtatgc cttcagcacg ccggtccggg
     1861 agctgctggg cccgctcgcc ggcctggccg acgcgatcag catcccggcg ctcctggtct
     1921 tcaccgcgat cctgatggcg ggcggcgcct tcgtggtgtg ggacatcgcg accgcgctgt
     1981 cgcgcgcggc gcagatcagc aacgaggaca acccggcgct gtccgccgag cccggccgca
     2041 aggacgagat cggcaccctc atgagctcct tcgcgcgaat gctcgccacc atccagcaac
     2101 agtcgcagga gatcaatgag ttcccccggc ggctcgacca gctcatccgc gaggccttcc
     2161 gcgactccct gaccagcctg ccgaaccggg ccctcttcat ggaccggctc tcgcacgccc
     2221 tcacgcgcgc cgagcgggcg ggcacgaacc tggccgtgct cttcctcgac ctggaccgct
     2281 tcaagatcct gaacgagacc ctcggtcacg aggtcggcga ccgcctgctg gtggaggtcg
     2341 ggcatcgcat cgccgcgtgc ctgcggcccg aggacacggt ggcccgcctg ggcggcgacg
     2401 agttcggcct gctcctcgag gacaccgccg acctggccgg cgccaccgcg gtggcggagc
     2461 gggtctccac cgagatccag cgtcccttcg tggtcgaggg ccgcgacgtg ctgatatcgg
     2521 ccagcatcgg catcgcgctg accgcgggcg ggtccatgca gcccgaggag gtgctccaca
     2581 acgcggacct cgcgatgtac caggccaagg ccgaggggcg cgggcgctac gagctctacc
     2641 agccgggact cagcgtctcc acccgcgagc ggctggatct gcagtcggac ctccgcaccg
     2701 cgggcgcccg ccaggagctg accctgcgct atcagccggt ggtgaccctc gcgaccctcc
     2761 gacccgtcga ggtcgaggcc ctcatccggt gggatcatcg gcgtcgcggc gccctcctcc
     2821 ccgccgattt catcgcgctc tccgaggaga ccggcttgat ggtgccgatg ggccagtggg
     2881 tcctgcgcga ggcctgccgc cagacccggg cctggcaggg agacggctcg gcgacgcccc
     2941 cgctggtggt gggcgtcaac ctgtcggcga gccagttcga gcgcgacgcg ctgcccgagg
     3001 agatcggcac catcctgcgc gagaccggcc tgcagccgag ccggctccag ctggagatca
     3061 gcgaggcggt cctgatgcgc gacgacccgc gcatgctcca ccgcctggag gccctcaagg
     3121 gcatcggggt gcggctcgcc atcgacgact tcggcaccgg atacgcctcc ctctcctacc
     3181 tcaagcgcct gccggtggac tgcctgaaga tcgaccgctc gctggtcaag ggcgtgggca
     3241 gcgacaccga ggacaccgcc atcgtccgtg cggtggtggc cctcgcccgc agcctgggca
     3301 tcgtggtgac cgcggagggc gtggagaccg ccgagcagct cttccagctg cgcgccctcg
     3361 gctgcgagca gggccaggga tactacttcg ctcggccggt ctccgccgac cgcttgcccg
     3421 agctgctgtc cagcctcgac gccgacgacc ggcacgggag tccctcatgt cttgggtagc
     3481 cctccgggcg ttgctgcgca ccatcctcat ccgtctgatc gggatcgcga tcttcaccgg
     3541 cgccctcggc ctcgggctcg cctggctcct cgacgtggcg gtctccgagc cggagtggac
     3601 cgagaccgcg gcgatcgccc agcgggcggt ggaccgcagc ggcctgccgg agctgctggc
     3661 cggccagagc gcgcgcagcc ccaagcgctg ggccgacgag agcgcccggt tcgtgcgcga
     3721 gcttccgggc gtcgtgcaga t
//
LOCUS       DATMCV010000015         5867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_732207, whole genome shotgun sequence.
ACCESSION   DATMCV010000015 DATMCV010000000
VERSION     DATMCV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732207"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VNU03_00465"
     CDS             complement(<1..663)
                     /locus_tag="VNU03_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP72945.1"
                     /translation="MNAPSIKELMATFIARDITDGEEVFVGTNLPVIRAGALLAHLHH
                     GPSMRVMVAHTRTNLYHTPVMEYFELLTDWRGARWAEAYFNDNEIVMYQKSRRDSVFF
                     VGALQIDPFGNSNLIGLGRDQRRLTLRGPGAIATTTNASYVRRFYLFVNEHSPRVFVP
                     RCDFVSTVGWGEGGADARRRLGLPGGGPRYCITPLAVLDFEETSKRLRLRSVHPGVTV
                     DEV"
     gene            complement(663..1574)
                     /locus_tag="VNU03_00470"
     CDS             complement(663..1574)
                     /locus_tag="VNU03_00470"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWP72946.1"
                     /translation="MSRSEAEAERQSLLVEEAALSTWLRDGMCVALGGLATACHAMIG
                     VRHIVRRGLRDLTVVGSAVGGLDVDLLIGAGCVRKVICPYVGAEALASIGPFYRAAAE
                     RGEIEVWECDEGQYYAGLQAAGQMLPFMPTRGGVGTSYPDVNPDLKLFADPLRGETLV
                     AVPAIAPDVALLHAAQADAYGNVQFVGTGYADRLLWRAADRTLVQVEKVVSNEEIRRA
                     PERTALLAHGVLRAPFGAHPFSSPGFYLEDRAHIGELVEAGRAFARTGDRGPFERYLD
                     RYVRGPADHVEYLEAVGLRRLLALGEY"
     gene            complement(1575..2126)
                     /locus_tag="VNU03_00475"
     CDS             complement(1575..2126)
                     /locus_tag="VNU03_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWP72947.1"
                     /translation="MPRAASLGERIRMSRLNRRLTLGQVAEKVGVSVSALSQLERDQF
                     NPTIATLKAIATTLDTTIGSLVAETSPLDRAVVRAGERKRLSPRRGITYQLLTPDLSG
                     HIELILSEYEVGAGTGEEAFAYSAEQCGLVLQGVAQVHLGDTVHRLRAGDSIRFDCSI
                     PHRIANAGRGRLRCLWAISPPTF"
     gene            2225..3697
                     /locus_tag="VNU03_00480"
     CDS             2225..3697
                     /locus_tag="VNU03_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HWP72948.1"
                     /translation="MARPKLVLAMMMHETNTFSPVPTPLASFRPLQGEAAITEFKDTN
                     TQLGGFLDVAQRAGAEIVLPVAGGAHPSGYVERAAYEDMVEAIVGAVRGGCDAVFLAL
                     HGAMVAEHVDDGEGELLRRIRAVAPRVPIAVGLDFHSHMTAAMVDHATVIAGYCTYPH
                     VDMGDTGQRAGRTMMRALRGEIEPVMVWGSLPMMTSTLVHAPSRQPMKDVMDMAMAAE
                     ASGAVLNASVFGGFPHSDIPHVSLSTVIVCDRRTDAGLALRDQLLAMAWERREEFLYR
                     GAPLVDQVAHARSLSEGPIVLVDHGDNTASGGTQDVMSVIEEVMRQGLTDVVAGPICD
                     PAGVRRLVEAGTAAGVTLPLGGRVDMPQINLLGKPLAVTGKVTRITDGQFTVTGPMAT
                     GTRVRMGRTAVLDTGAMQIVVSEGRSEPFDTGVFTHCGIDPRRKKYVLIKSRQHFRAG
                     FEPIARHIVLCDGDGVTSSDLRLFTFRNRRRPLFPFERDL"
     gene            3722..4807
                     /locus_tag="VNU03_00485"
     CDS             3722..4807
                     /locus_tag="VNU03_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP72949.1"
                     /translation="MDYGLFTMPSHPPERKLFDGHQWDLQQLRWADELGFSEAWIGEH
                     HTSPWEPHPSPDLLVAQALLQTKRMRIGPGGFLMPYHHPAELANRLAILDHMAQGRLN
                     FGVAASGLPSDWSMFNVDGMSGQNREMTRESLDIILRLWSDEPEFDFKGKFWHVTKTG
                     TMFETLKPHIYPFQKPHPPIGVAGLSKGSDTLKLAGERGYWPMSLNLNPTYVGSHWEA
                     VEQGAERGKRTAKRADWRMVREVFVADTDAEAMRHSVGGMMGRMMTEYFLPLLGAFAF
                     TEFLKHDPSVPDSDVTPEYCAKHNWLVGSPDTVARKLNDIYEEVGGFGTLLLFCFDYS
                     ENPKAWHHSMELLAKEVMPRFKNVVPK"
     gene            4811..>5867
                     /gene="alr"
                     /locus_tag="VNU03_00490"
     CDS             4811..>5867
                     /gene="alr"
                     /locus_tag="VNU03_00490"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWP72950.1"
                     /translation="MDPGALRPTVVEVSLARLAENFRAIQAAVAPAVVMPIVKANAYG
                     HGLVPVARHLASLGARSLGVAFLEEAVALREAGLTLPILVMGGIFGDQIPIFLRHDLT
                     LTASSIDKLKQIDETAGQMGVGARVHLKIDTGMERIGVHYYSARGLLEQAAECRHCQV
                     EGIYSHFANADAADLTSARLQLARFLEVLEWYDKQGVKPPVRHMANSGAVLQLRESHL
                     DMVRPGILLYGVYPSDEVRKTIAVGPALSLRSRVVYFKVVTPGHPVSYGSTWESDHPV
                     RVVTVPVGYGDGYFRALSNVAHVLIRGRRYPVVGRVCMDQIMVNIEWETAYNEDVVTL
                     LGADGDEIITCEHLAEWA"
BASE COUNT          890 a         2029 c         2099 g          849 t
ORIGIN      
        1 cacctcgtcc accgtgaccc ccggatgaac cgatcggagg cgcagccgct tgctcgtctc
       61 ctcgaagtcg agcaccgcca gcggggtgat gcagtagcgc ggcccgcccc cgggaagccc
      121 caggcggcgg cgggcgtccg cgccgccctc cccccagccc accgtgctca cgaagtcgca
      181 gcgggggacg aagacccgcg gggagtgctc gttgacgaac aggtagaagc gccgcacgta
      241 ggaggcgttg gtggtcgtgg cgatcgcgcc gggcccgcgg agggtgagcc ggcgctggtc
      301 gcggccgagg ccgatcaggt tcgagttgcc gaacgggtcg atctggagcg cgcccacgaa
      361 gaacaccgag tcccgccggc tcttctggta catcacgatc tcgttgtcgt tgaagtaggc
      421 ctcggcccag cgggctccgc gccagtcggt gagcagctcg aagtactcca tgaccggcgt
      481 gtggtagagg ttggtccggg tgtgggcgac catcacccgc atgctcggcc cgtggtgaag
      541 atgggccagc agcgcgccgg cccggatcac gggcaggttg gtgcccacga agacctcctc
      601 cccgtccgtg atgtcccggg cgatgaaggt cgccatcagc tccttgatcg acggcgcgtt
      661 catcagtact cgccgagcgc caggagacgg cgcaggccga ccgcttccag gtactccacg
      721 tgatcggcgg ggccgcggac gtagcggtcc aggtagcgct cgaacgggcc gcggtcgccg
      781 gtccgcgcga acgcgcgccc ggcctcgacc agctctccga tgtgggcgcg gtcctcgaga
      841 tagaagccgg ggctcgagaa gggatgggcg ccgaagggag cgcgcaggac cccgtgggcg
      901 agcagcgcgg tgcgctcggg cgcccgccgg atctcctcgt tcgagaccac cttctcgacc
      961 tggaccaggg tgcggtccgc ggcgcgccac aggagccgat cggcgtagcc ggtgcccacg
     1021 aactgcacgt tgccgtaggc gtcggcctgc gcggcgtgga gcagcgccac gtccggcgcg
     1081 atggcgggca ccgcgaccag ggtctcgccg cgcaggggat cggcgaagag cttcagatcg
     1141 gggttcacgt cgggatagga ggtgccgacg cctccgcggg tgggcatgaa gggcagcatc
     1201 tggccggccg cctgcagccc ggcatagtac tgcccctcgt cgcactccca gacctcgatc
     1261 tccccgcgct cggcggccgc gcggtagaag ggcccgatcg aggccagggc ctccgccccc
     1321 acgtaggggc agatgacctt gcgcacgcac ccggccccga tgagcaggtc cacgtcgagc
     1381 ccgcccaccg cggagccgac caccgtgagg tcgcgcagcc cgcggcggac gatgtggcgg
     1441 acgccgatca tggcgtggca ggcggtggcg aggcccccga gggcgacgca cataccgtcg
     1501 cggagccacg tcgatagcgc ggcctcctcc accaggagcg actggcgctc cgcctcggcc
     1561 tcgctccggc tcatttagaa ggtcgggggg ctgatcgccc acaggcagcg gagccggccg
     1621 cggcccgcgt tggcgatgcg atgggggatg gagcagtcga agcggatgct gtcgcccgcc
     1681 cggagccggt gcaccgtgtc gcccaggtgg acctgggcca cgccctggag cacgagcccg
     1741 cactgctcgg cggagtacgc gaaggcctct tccccggtcc cggcgccgac ctcgtactcg
     1801 ctcaggatga gctcgatgtg cccggagaga tcgggagtca gcagctggta ggtgatgccg
     1861 cggcgggggg agagccgctt gcgctcgccg gcccgcacca ccgcccggtc gagcgggctc
     1921 gtctcggcca cgaggctgcc gatggtggtg tcgagcgtgg tggcgatcgc cttgagggtg
     1981 gcgatggtgg gattgaactg atcgcgctcg agctggctga gcgcgctcac gctcaccccc
     2041 accttctcgg cgacctggcc gagggtcaat ctccggttca atcgggacat ccggatacgc
     2101 tccccgaggc tggccgcgcg cggcatgggc gcaccctatc cagcaccact gtaaaagtca
     2161 agtgacactg aaacaggcga gaaccggggg gcgccgcgag gcccggctgg ggtatgctgc
     2221 ccgcatggcc cgcccgaagc tcgtcctcgc gatgatgatg cacgagacga acaccttctc
     2281 gccggtgccc accccactcg cgtcgttccg ccccttgcag ggcgaggccg ccatcaccga
     2341 gttcaaggac accaacaccc agctcggcgg cttcctcgac gtggcccagc gggccggcgc
     2401 cgagatcgtg ctgccggtgg cgggcggggc gcacccgtcg ggctacgtgg agcgggcggc
     2461 ctacgaggac atggtggagg ccatcgtggg cgcggtgcgg ggcggctgcg acgcggtgtt
     2521 cctcgcgctg cacggggcga tggtggccga gcacgtcgac gacggcgagg gcgagctgct
     2581 gcggcgcatc cgcgcggtgg cgccgcgggt gccgatcgcg gtgggcctcg acttccactc
     2641 ccacatgacc gcggccatgg tcgaccacgc gacggtcatc gcgggctact gcacctaccc
     2701 ccacgtggac atgggcgaca ccgggcagcg cgccgggcgc accatgatgc gcgcgctgcg
     2761 cggggagatc gagccggtca tggtctgggg ctcgctcccc atgatgacga gcaccctggt
     2821 ccacgccccc tcgcggcagc ccatgaagga cgtcatggac atggcgatgg ccgcggaggc
     2881 ctcgggcgcg gtgctcaacg cctccgtctt cggcggcttc ccgcactcgg acatcccgca
     2941 cgtctcgctg tccaccgtga tcgtctgcga tcggcggacc gacgcggggc tcgcgctgcg
     3001 cgaccagctg ctcgcgatgg cgtgggagcg gcgcgaggag ttcctctacc ggggcgcgcc
     3061 cctggtcgac caggtcgcgc acgcccgatc gctgagcgaa ggaccaatcg tgctggtgga
     3121 ccacggcgac aacaccgcgt ccggcggcac ccaggacgtg atgagcgtga tcgaggaggt
     3181 catgcgccag ggactcaccg acgtggtggc cgggcccatc tgcgacccgg ccggcgtgcg
     3241 gcggctggtg gaggcgggga ccgcggcggg cgtgaccctg ccgctcggcg gccgcgtcga
     3301 catgccccag atcaacctgc tcggcaagcc gctggccgtg accggcaagg tcacccgcat
     3361 caccgacggg cagttcaccg tcaccgggcc gatggccacc ggcacccggg tgcgcatggg
     3421 gcgcaccgcg gtgctcgaca ccggggccat gcagatcgtg gtgtcggagg ggcgcagcga
     3481 gcccttcgac accggggtct tcacccactg cgggatcgac ccgcgccgca agaagtacgt
     3541 cctgatcaag tcgcgccagc acttccgggc cgggttcgag ccgatcgccc ggcacatcgt
     3601 cctctgcgac ggtgacggcg tgaccagctc cgacctccgg ctcttcacgt tccggaaccg
     3661 gcggcggccg ctctttccct tcgaacgaga tctgtagcag cgatctgtca ggaggcttgc
     3721 catggactac ggtctcttca cgatgccgtc gcacccgccg gagcggaagc tcttcgacgg
     3781 gcaccagtgg gatctccagc agctgcgctg ggccgacgag ctgggcttca gcgaggcctg
     3841 gatcggtgag caccacacct cgccgtggga gccgcatccc tcgccggatc tcctggtggc
     3901 ccaggcgctg ctccagacca agcgcatgcg catcgggccc ggcggcttcc tgatgcccta
     3961 tcaccatccc gccgagctgg ccaaccgcct cgcgatcctc gaccacatgg cccagggccg
     4021 gctgaacttc ggggtcgccg cgagcggcct gccgagcgac tggtcgatgt tcaacgtgga
     4081 cggcatgtcg gggcagaacc gcgagatgac ccgcgagtcg ctcgacatca tcctgcgcct
     4141 ctggagcgac gagcccgagt tcgacttcaa gggcaagttc tggcacgtca ccaagaccgg
     4201 gaccatgttc gagaccctca agccccacat ctatcccttc cagaagccgc acccgcccat
     4261 cggggtggcc gggctcagca agggctcgga cacgctgaag ctggcgggcg agcgcgggta
     4321 ctggccgatg agcctgaacc tcaacccgac ctacgtgggc agccactggg aggcggtgga
     4381 gcagggcgcg gagcggggca agcggaccgc caagcgcgcg gactggcgca tggtccgcga
     4441 ggtgttcgtg gccgacaccg acgcggaggc gatgcggcat tcggtgggcg gcatgatggg
     4501 ccgcatgatg accgagtact tcctgccgct cctgggcgcc ttcgcgttca ccgagttcct
     4561 gaagcacgac ccgagcgtgc ccgactcgga cgtgaccccc gagtactgcg ccaagcacaa
     4621 ctggctggtg ggctcgcccg acacggtggc gcggaagctc aacgacatct acgaggaggt
     4681 gggcggcttc ggcaccctgc tcctgttctg cttcgactac agcgagaacc cgaaggcgtg
     4741 gcaccactcg atggagctgc tggccaagga ggtcatgccc cgcttcaaga acgtggtgcc
     4801 caagtaaccg gtggatccgg gcgcgctgcg gccgacggtc gtcgaggtca gcctggcccg
     4861 tctcgcggag aacttccgcg cgatccaggc cgcggtggcg ccggcggtgg tgatgcccat
     4921 cgtcaaggcc aatgcctacg gccacgggct cgtgcccgtt gcccgccatc tggcctcgct
     4981 gggggcgagg tcgctcggcg tcgccttcct cgaggaggcg gtggccctcc gcgaggcggg
     5041 cctgacgctg ccgattctag tgatgggggg catcttcggt gaccagatcc cgatcttcct
     5101 gcgccacgac ctcaccctga ccgcgtcctc catcgacaag ctcaagcaga tcgacgagac
     5161 cgccgggcag atgggggtgg gggcgcgggt gcacctcaag atcgacaccg gcatggagcg
     5221 gatcggcgtg cactactaca gcgcgcgcgg gctgctcgag caggccgccg agtgccgcca
     5281 ctgccaggtg gagggcatct actcgcactt cgccaacgcg gacgcggccg atctcacctc
     5341 ggcgcgcctg cagctcgcgc gcttcctcga ggtgctcgag tggtacgaca agcagggcgt
     5401 gaagccgccg gtgcgccaca tggccaactc cggcgcggtg ctgcagctgc gcgagagcca
     5461 tctcgacatg gtgcggccgg ggatcctgct ctacggggtc tatccctcgg acgaggtgcg
     5521 caagacgatc gcggtcgggc ccgccctgtc gctgcgctcg cgcgtcgtct acttcaaggt
     5581 ggtcacgccc gggcacccgg tcagctacgg ctcgacctgg gagtccgatc acccggtgcg
     5641 ggtggtcacg gtgccggtgg gctacggcga cggctacttc cgggcgctct ccaacgtggc
     5701 gcacgtgctg atccgcggcc gccggtaccc ggtggtgggg cgcgtctgca tggaccagat
     5761 catggtcaat attgaatggg agacggccta caacgaggac gtggtcacat tgctcggggc
     5821 cgacggcgac gagatcatca cctgcgagca tctcgccgaa tgggccg
//
LOCUS       DATMCV010000016         3281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_200200, whole genome shotgun sequence.
ACCESSION   DATMCV010000016 DATMCV010000000
VERSION     DATMCV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200200"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /locus_tag="VNU03_00495"
     CDS             complement(<1..1892)
                     /locus_tag="VNU03_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HWP72951.1"
                     /translation="MSVRSSDEGEPIAVIGLGCRFPGAPGPGAFWSLLERRAEAVGPL
                     PAERQGAGDGARVRGGFLADVDRFDARFFGISPREAERLDPQQRILLEVTWEALEDAG
                     LVSERLDGSTTGVFVGMWINDYESRMFAETGRVDFHMTTGSGRYTASGRMSHLFGLRG
                     PSVTVDTACSSSLVAAHLACQSLWSGESEMAIAAGVNVILQPHITAAYSQAGMLAADG
                     RCKFGHASADGYVRSEGVGVVVLKRLSRARADRDPVHAVILGSAVNNDGHTGGSLGTP
                     GRAGQEDLLRKAYRRAGVRPAAVGYVEAHGTGTRAGDPVELSALGVILGEERSTDRPC
                     LVGSVKTNIGHTEGAAGIAGLIKVVLSMQHGIIPASLHCETPTPAVAWDTMRVGIATS
                     ATPWPAGFRRIAGVSSFGIGGTNAHVVLAAVEDGAGPRESGESRIPAPGAETLLPLSV
                     RSADVLREQARRYADYLTGPGVAPLADVAYTAALRRTHHPHRLAVVGRDHREIGHRLA
                     TFAETGSAVGVVAGAGTGASQVVFVFPGQGSQWVGMGRGLLETEPAFREAIVRCERVI
                     APLTGWSLLAALRRDELTPERIDLIQPALFAIQVALAEQWRAWGVEPAAVVGHSMGEV
                     AAAHVAG"
     gene            complement(2472..2548)
                     /locus_tag="VNU03_00500"
     tRNA            complement(2472..2548)
                     /locus_tag="VNU03_00500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2512..2514),aa:Arg,seq:tct)
     gene            complement(2566..3228)
                     /locus_tag="VNU03_00505"
     CDS             complement(2566..3228)
                     /locus_tag="VNU03_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWP72952.1"
                     /translation="MPAFPEHRREPEARQGFITTEQHQAIRTYLRAHEDGAAYADALD
                     LSMLTGWRKGQTLHLRWENVHKDEIRVAGDVTKNEASHVIPIVPALAEIIERRRAARV
                     VGSAWVFCRKDGSRIGDFEKAWKSATKAAGCEGVLYHDSRRSGVRNLTIAGVPQSVAM
                     KISGHKTDAVFRRYNIVTTSDMRKALEAVSADSIAAKVAPQKRPQKGPKGRVVAMGGR
                     RK"
BASE COUNT          512 a         1142 c         1120 g          507 t
ORIGIN      
        1 ccggccacgt gcgcggccgc gacttcgccc atgctgtggc ccaccaccgc cgccggctcg
       61 actccccacg cgcgccactg ttcggcgaga gccacctgga tcgcgaagag ggctggctgg
      121 atgaggtcga tccgctcggg cgtcagctcg tcccggcgga gcgccgcgag cagcgaccat
      181 cccgtcagcg gagcgatcac gcgctcgcag cgaacgatgg cctcacggaa ggcgggctcc
      241 gtctcgagca gcccacggcc catgccgacc cactgcgagc cctgccccgg gaagacgaac
      301 acgacctgcg atgcgccggt gccggctccc gccaccactc cgaccgcgga gcccgtctcg
      361 gcgaacgtgg cgagacgatg cccgatctcc cggtggtcgc ggccgacgac cgccagccga
      421 tgcgggtgat gcgtccggcg gagggccgcg gtgtaggcga cgtcggccag cggggcgacg
      481 ccgggtccgg tgaggtagtc cgcgtagcgg cgcgcttgct cgcgcaggac gtccgccgac
      541 cggacggaga gcggcagcag cgtctcggcg ccaggcgcgg ggatgcgact ctcaccgctc
      601 tcgcgcgggc cggcaccgtc ctcgacggcg gcgagcacca cgtgggcatt ggtcccgccg
      661 atgccgaagg agctgacacc ggcgatccgg cggaagccgg ccggccaggg cgtggcggag
      721 gtcgcgatcc cgacgcgcat cgtgtcccag gccaccgcgg gcgtcggtgt ctcacagtgg
      781 aggctggccg gaatgatgcc gtgctgcatc gagagaacga ccttgatcag gccggcgatc
      841 ccggcggctc cttccgtgtg accgatgttg gtcttcacgg atccgaccag gcacggccga
      901 tcggtggagc gctcctcgcc caggatcacg ccgagcgcgc tcagctccac gggatccccc
      961 gcgcgagttc cggtgccgtg cgcctcgacg tagccgacgg ccgccgggcg gacgccggcg
     1021 cgccggtacg ccttccgcag gaggtcctcc tggccggcgc ggcccggcgt gccgagcgag
     1081 cccccggtat ggccgtcgtt gttcaccgcg ctgcccagga tcaccgcgtg gaccggatct
     1141 cggtcggcgc gggcgcgcga gagccgcttg agcaccacca ccccgacccc ctcgctccgc
     1201 acgtatccat ccgcggaggc gtggccgaac ttgcagcgtc cgtccgcggc gagcatgcca
     1261 gcctgcgagt acgcggcggt gatgtgaggc tggaggatca cgttcacgcc ggccgcgatc
     1321 gccatctcgc tctcgccgct ccacaggctc tgacaggcga ggtgcgccgc cacgagcgac
     1381 gaggagcacg cggtgtcgac ggtcacgctc ggcccgcgca acccgaaaag gtgcgacata
     1441 cggcccgagg cggtgtagcg accgctgccg gtggtcatgt ggaaatccac gcgaccggtc
     1501 tccgcgaaca tccgcgactc gtagtcgttg atccacatgc cgacgaacac gccggtcgtg
     1561 ctgccgtcga gccgctcgga caccagcccc gcgtcctcga gcgcctccca cgtgacctcg
     1621 agcaggatcc gctgctgcgg gtcgagacgc tccgcctcgc gcggcgagat cccgaagaac
     1681 cgcgcgtcga accggtcgac gtccgcgagg aacccgccgc gcaccctggc gccgtcgccc
     1741 gccccctggc gctcggccgg cagcgggccc acggcctcgg cgcggcgctc gagcagcgac
     1801 cagaaggccc cgggccccgg ggctcccggg aaccggcagc cgaggccgat cacggcgata
     1861 ggctcgcctt cgtcgctcga acgaacgctc atgggctcca tgtttttggg ctgaggttca
     1921 actgagctgg ggtgcgggct cagggcaaac gaatcgccag gaggagggtg tgcaagtcca
     1981 aagccatggg ggggtgctag aaacgccagc aggcttcacc gcttagtggg tcagcagctc
     2041 gggcagaacc tgcaactgat atgcgcggga cggacttttt cgtgcaaata gtgcatgtgg
     2101 gaaatctagg tactacttca aattcgagca tttagcttag ccgccgggcc ttcgagtggg
     2161 accggctaac taagcagatg agtaccctag ccgagcaagc atttcagctg tttcagcacc
     2221 tcgctgcggg ctcggaccat ggcatcggga ttccatccaa tcgtgacacc tcggcggcca
     2281 cacgtgggca tctcgtcgag gttatccacc ggaccgagca ggctgatgcg ccacaatccc
     2341 gcaagcgaga atcccatcgg cgcgatccgc tgcgcgtcga gcgcgggata actggagaga
     2401 atccgcggct cgagttggtt tccgcgccac aggcggccgc atctcgaatg tgtctgccgc
     2461 cgaatcagaa gtggcgcgcc tggagggatt cgaacccacg accctcggct tagaaggccg
     2521 atgctctatc caactgagct acaggcgcat taccaattat ttccgctatt tccggcgacc
     2581 acccatggcg acaactcgcc ccttggggcc cttttggggc ctcttttggg gggccacttt
     2641 ggcggcgatg gagtcggcgg ataccgcctc cagggccttc cgcatgtcgc tagtggtgac
     2701 gatgttatac ctgcgaaaca ccgcgtcggt cttgtgcccg ctgatcttca tggcgacgga
     2761 ctggggcacg cccgcgatcg tgaggttgcg gaccccgctc cgccgggagt cgtggtagag
     2821 gacgccttca cacccggccg ccttggtggc gctcttccag gccttctcga agtccccgat
     2881 gcgggagccg tccttccggc agaacaccca ggcggagccg actacgcgag ccgcccggcg
     2941 gcgctcgatg atctccgcga gggccgggac gatcgggatg acgtggctgg cctcgttctt
     3001 ggtgacatca cccgccacgc ggatctcgtc cttgtggacg ttctcccacc tgagatggag
     3061 cgtctggccc ttgcgccacc cggtgagcat ggacagatcg agtgcgtccg cgtacgcggc
     3121 accgtcctcg tgcgcgcgga gataggtgcg gatcgcctgg tgctgctcag tcgtgatgaa
     3181 gccctggcgc gcctcgggct ccctccgatg ctcggggaac gcgggcatgg tggccaagac
     3241 tctcgcgcgg atggccagcc tgtagctgtg cttcaacgcg g
//
LOCUS       DATMCV010000017         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_931577, whole genome shotgun sequence.
ACCESSION   DATMCV010000017 DATMCV010000000
VERSION     DATMCV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931577"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1558
                     /gene="purF"
                     /locus_tag="VNU03_00510"
     CDS             134..1558
                     /gene="purF"
                     /locus_tag="VNU03_00510"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWP72953.1"
                     /translation="MSAEPTALDLDDKFHDECGLYGVWNHAEAANVAYLGLYALQHRG
                     QESAGIASTDGHHFQVEKAMGWVADVFSRERLKRLPGHRAIGHVRYSTAGSSNLRNAQ
                     PITGTTAHGPVAIAHNGNLVNAEALREELEKDGAVFQSSSDTEVILHLIARSEGATLA
                     DQLARALTRVRGAYTLLLLTPDSMIGVRDPSGFRPLTLGSLGDAWVLASETCALDLME
                     AKVERDVEPGEILIVDERGLTSVKPFRPADRLQCVFEYVYFARPDSVLWGRNVHMVRK
                     ALGHQLAREYPVAADLVIPVPDSGVGAALGFSEESGLPYDSGLVRNHYVGRTFIEPQQ
                     GIRHFGVKVKLNPNREVLDGKRVVVVDDSIVRGTTSRKIVKMIRAAGAREVHVRISSP
                     PIQWPCYYGIDTPTRRELIGSSHKVEEIQRYLGADSLGYLSLEGMLKATGSDPNQFCH
                     ACFTGQYQVGFESEELAQLRLFDS"
     gene            1565..>2161
                     /gene="purM"
                     /locus_tag="VNU03_00515"
     CDS             1565..>2161
                     /gene="purM"
                     /locus_tag="VNU03_00515"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWP72954.1"
                     /translation="MDPLTYRAAGVDIQAGDLAVRRIAPLAASTRRPEVLGGLGAFAA
                     FCRVPVGYREPVLVSSTDGVGTKLKIAIMADRHDTVGVDLVAMGVNDLLVHGAEPLFF
                     LDYLAVHRVDPDRVETLVAGIAEGCRQAGCALVGGETSEMGALYAPGDYDLAGFAVGV
                     VEQSLIITGEDVAIGDRVLGLASSGLHSNGYTLAREAIF"
BASE COUNT          311 a          756 c          763 g          331 t
ORIGIN      
        1 gagttccgcg taccgtggcg gttcatggga acggtcggcg gcgagcggct ggtcgtcaag
       61 tcgggtggga cgcgtctcgt ggatctggac ctgcaccgca tctccggcgc ctggagggac
      121 ggctttgagc gacatgtcag ctgagccgac ggcactcgac ctggacgaca agttccacga
      181 cgagtgcggc ctctacggcg tctggaacca cgccgaggcc gccaacgtcg cctatctcgg
      241 gctctacgcg ctgcagcacc gcggacagga gtcggccggg atcgcctcga ccgacgggca
      301 tcacttccag gtcgagaagg cgatgggctg ggtggcggac gtcttcagcc gggagcggct
      361 caagcgcctg cccggccacc gcgcgatcgg ccacgtccgc tactccaccg cgggcagctc
      421 gaacctgcgt aacgcccagc ccatcaccgg caccaccgcg cacggcccgg tggccatcgc
      481 ccacaacggc aatctcgtga acgcggaggc gctgcgggag gagctcgaga aggacggcgc
      541 ggtcttccag tcgtcgtccg acaccgaggt gatcctgcac ctcatcgcgc ggtccgaggg
      601 ggcgacgctc gccgaccagc tcgcccgggc gctcacccgc gtgcggggcg cctacaccct
      661 gctgctgctg acccccgaca gcatgatcgg ggtgcgcgac ccgtccggct tccggcccct
      721 caccctgggc agcctcgggg acgcctgggt gctcgcctcc gagacctgcg cgctcgacct
      781 catggaagcc aaggtggagc gcgacgtcga gccgggcgag atcctgatcg tcgacgagcg
      841 cggcctcacc tcggtcaagc cgttccggcc cgccgatcgg ctgcagtgcg tgttcgagta
      901 cgtctacttc gcccggccgg actcggtcct gtgggggcgc aacgtgcaca tggtgcgcaa
      961 ggcgctcggg catcagctgg cccgggagta cccggtggcc gcggacctgg tcatcccggt
     1021 gccggactcc ggggtgggag ccgcgctcgg cttctcggag gagtccgggc tgccgtacga
     1081 cagcgggctc gtccgcaacc actacgtcgg ccgcaccttc atcgagcccc agcagggcat
     1141 ccgccacttc ggggtcaagg tgaagctcaa cccgaaccgt gaggtgctgg acggcaagcg
     1201 ggtggtggtg gtcgacgact cgatcgtgcg cggcaccacc agccgcaaga tcgtcaagat
     1261 gatccgcgcc gcgggggcgc gcgaggtgca cgtgcgcatc tcgtcgccgc cgatccagtg
     1321 gccgtgctac tacggcatcg acacgcccac ccggcgcgag ctgatcgggt cgagccacaa
     1381 ggtcgaggag atccagcgct acctgggcgc ggactccctc ggctatctct cgctcgaggg
     1441 catgctcaag gccaccgggt cggacccgaa tcagttctgc cacgcgtgct tcaccggcca
     1501 gtaccaggtc ggcttcgagt ccgaggaact ggcgcagctc aggttgttcg actcgtaggg
     1561 ggtaatggat ccgctgacgt atcgggcggc cggcgtggac atccaggccg gcgaccttgc
     1621 cgtgcggcgc atcgcgccgc tggcggcgtc cacccggcga ccggaggtgc tgggtggcct
     1681 cggcgccttc gcggcgttct gtcgcgtccc ggtcggttat cgcgagccgg tgctcgtctc
     1741 ctccaccgac ggggtcggca ccaagctcaa gatcgcgatc atggccgatc gccacgacac
     1801 ggtgggcgtc gatctcgtcg cgatgggcgt caacgacctg ctcgtgcacg gggccgagcc
     1861 gctcttcttc ctcgactacc tcgcggtgca ccgggtcgac cccgaccggg tcgagacgct
     1921 cgtggccggc atcgcggagg gctgccgcca ggcgggctgc gcgctggtgg gcggggagac
     1981 ctcggagatg ggcgccctct acgcgcccgg cgactacgac ctggccggct tcgcggtcgg
     2041 ggtggtggag cagagcctga tcatcaccgg cgaggacgtg gcgatcggcg accgggtgct
     2101 gggccttgcc tcctcgggac tccactccaa cggctacacg ctcgcgcgcg aggccatctt
     2161 c
//
LOCUS       DATMCV010000018         4307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_533162, whole genome shotgun sequence.
ACCESSION   DATMCV010000018 DATMCV010000000
VERSION     DATMCV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533162"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..980)
                     /locus_tag="VNU03_00520"
     CDS             complement(78..980)
                     /locus_tag="VNU03_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP72955.1"
                     /translation="MATRPLGVEAPAVALALRRPGAWTVLGRFCRKKPLGAIGGFLML
                     VILFTAVFADQLQTYDPIATDAGQSLARPGADHWLGSDHLGRDIYSRIVHGARVSLIV
                     GLASTLMGSVLGGIIGLLSGYVGGKTDLVTQRVMDILQGLPLLVLALVMSAALGPSIP
                     NVVIAISIPIIPRAARVIRASVLSIREMQYVEAARAVGLRHVRIAFRHILPNTIGPFI
                     VLCTAQLGSAILVEAALSFLGLGVPEPYPSWGRMLSVSAAEYAQKAPWLVLFPGLAIS
                     LAVFGSNLLGDALRDTLDPRLRGA"
     gene            complement(970..1923)
                     /locus_tag="VNU03_00525"
     CDS             complement(970..1923)
                     /locus_tag="VNU03_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP72956.1"
                     /translation="MKTYIAQRLGIGFLTLLGMSIVIFGMLRLAPGDIVDILFSTGGY
                     VNPAERAAIEKELGLDRPVWVQYVEWIRQIVTGDLGKSYRYDLPAWEIIRPLIPVTVE
                     LAVLSLIVSVALGVPFGVISAVRQDTGLDYALRVFSLAGLSMPSFWLGMVIILSLVAW
                     FGWIPPVTYVSPRENFLLHCVQFALPSLAVGYRSSALIMRITRSSLLEVMREDYIRTA
                     RAKGQSGRVVIWRHALKNAILPVVTVIGIEFAFLIGGLVVTETVFNLPGLARFLVEAI
                     LWRDYPIVQNLVMFIAIVVILSNLFVDMLYGFLDPRVRYGH"
     gene            complement(1949..3559)
                     /locus_tag="VNU03_00530"
     CDS             complement(1949..3559)
                     /locus_tag="VNU03_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP72957.1"
                     /translation="MKRSRTFAIAISLGLAAVVATTVPSSAQAPKSGGHLNLMQREDL
                     SQGFAVHETSTIATSFPSMPCFNNLVVFDQTKRAETVDNLVGELAEKWSWQDNYRNLV
                     FFLRKDVKWHDGQPFTSKDVKFTFDMVREAPEATTKLRINPRKDWYTNVEKIEAPDPY
                     TVVFHLKRPQPGLLVMLASGYSPVYAAHVNPATYRTGCIGTGPFKVKEWRKGEFVDYV
                     KNPDYFMKGRPYLDSLRYVVIKERGTRTAALQAGQLDVSFPGETTKTAAEQLKKAVPG
                     LVITPVAQAVSDNIILNTKKPPFDNPKVRLAVAYAVDRRALISAVHQGGAVPGASQQP
                     KPYGVWGLLDKDLAALPGYGKPADMKAHARKLLAEAGVTPQNPLRVEMSTRAIAMYVD
                     LASFVINELKQVGIEATLKQVETAQWHPMATRGEYQIGANLTGLGVDEPDANYYENYA
                     CGSPRNYSQYCSEEVMKLIEQQSQELNPQKRLALVSTLQKKVEADAARPLLDWRLDYF
                     TVWPHVKNLVPHDNIYNFGRMADVWRDK"
     gene            complement(3786..>4307)
                     /locus_tag="VNU03_00535"
     CDS             complement(3786..>4307)
                     /locus_tag="VNU03_00535"
                     /inference="COORDINATES: protein motif:HMM:NF017277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HWP72958.1"
                     /translation="SAIARSGEEPDMPALHGHSIPAPTPGAIVLPGRVLGLDDGASRG
                     IALRPLPRRSPLALLRADPRQAVEHDLIRCLVCGSAFRQLTNTHLRNHGMTADEYKRQ
                     FGYNRGRPLMCHALQRLYTERAVKIGLAARIRERPILARPELRRQGGARTIALEEILT
                     RREVRERGRPSSY"
BASE COUNT          699 a         1411 c         1542 g          655 t
ORIGIN      
        1 agcagagatc gtcgttggta gccatggaag ctcccctcac cctgccctct cccccgaggg
       61 gagagggggg gtcgtggtta ggcgccgcgg aggcgcggat ccagtgtgtc gcggagcgcg
      121 tcgccgagca ggttggagcc gaagacggcc aggctgatgg ccaggcccgg aaagaggacg
      181 agccacggcg ccttctgcgc gtattccgcg gcggagaccg agagcatccg gccccacgac
      241 ggatacggct ccgggacgcc cagcccgagg aacgagagcg cggcctcaac caggatcgcg
      301 ctgccgagct gggcggtgca cagcacgatg aaggggccga tggtgttggg caggatgtgg
      361 cggaacgcga tgcgcacgtg gcgcagcccc accgcgcgcg ccgcctccac gtactgcatc
      421 tcgcggatcg agagcacgct ggcccggatg acccgggcgg cccgcgggat gatcgggatc
      481 gagatcgcga tcaccacgtt cgggatcgac ggcccgagcg cggccgacat cacgagggcc
      541 agcaccagca gcggcagccc ctgcaggatg tccatcaccc gctgggtgac gaggtcggtc
      601 ttgcccccga cgtagccgga gaggagcccg atgatcccgc ccagcaccga gcccatgagg
      661 gtggaggcca gccccacgat gagcgagacc cgcgcgccgt gcacgatccg ggagtagatg
      721 tcgcggccga ggtggtcgga gccgagccag tgatcggcgc ccgggcgggc caggctctga
      781 cccgcgtcgg tggcgatggg gtcgtaggtc tggagctggt cggcgaacac cgcggtgaac
      841 aggatcacca gcatcaggaa gccgccgatc gcccccagcg gcttcttgcg gcagaaccgg
      901 ccgagcaccg tccacgcccc gggccggcgc agggccagcg cgaccgccgg cgcctcgacg
      961 ccgaggggcc tagtggccat agcggacccg cgggtcgagg aagccgtaga gcatgtccac
     1021 gaagaggttc gagaggatca ccacgatcgc gatgaacatc accaggttct gcacgatcgg
     1081 gtagtcccgc cagaggatcg cctccaccag gaagcgtgcg agcccgggca ggttgaagac
     1141 ggtctcggtc accacgaggc cgccgatgag gaacgcgaac tcgatcccga tcacggtcac
     1201 caccggcagg atcgcgttct tgagcgcgtg gcgccagatg acgacgcggc cggactggcc
     1261 cttggcccgc gcggtgcgga tgtagtcctc gcgcatcacc tcgagcagcg aggagcgcgt
     1321 gatgcgcatg atcagggccg aggagcggta gcccaccgcg agcgagggca gcgcgaactg
     1381 cacgcagtgc agcaggaagt tctcgcgcgg cgacacgtag gtgaccggcg ggatccagcc
     1441 gaaccacgcg accaggctga ggatgatcac catgcccagc cagaacgacg gcatgctgag
     1501 gccggccagg ctgaacaccc gcagcgcgta gtcgagcccg gtgtcctggc ggaccgcgct
     1561 gatcacgccg aagggcacgc ccagcgccac cgacacgatg agcgacagca ccgccagctc
     1621 gacggtgacc gggatgagcg ggcggatgat ctcccaggcg ggcagatcgt agcgatacga
     1681 cttgccgaga tcgccggtca cgatctggcg gatccactcc acgtactgca cccacaccgg
     1741 ccggtccagc ccgagctcct tctcgatggc cgcgcgctcg gccgggttca cgtagccccc
     1801 ggtggagaac aggatgtcca cgatgtcgcc gggggccagg cgcagcatgc cgaagatcac
     1861 gatcgacatg ccgagcaggg tcaggaaccc gatgcccagc cgctgcgcga tgtacgtctt
     1921 cacggtccgg cccccaggga cgggcgggtt acttgtcccg ccagacgtcg gccatgcggc
     1981 cgaagttgta gatgttgtcg tggggcacga ggttcttcac gtgcggccac accgtgaagt
     2041 agtcgagccg ccagtcgagc agcgggcgcg ccgcgtccgc ctccaccttc ttctggagcg
     2101 tgctcacgag ggcgaggcgc ttctgcgggt tcagctcctg cgactgctgc tcgatcagct
     2161 tcatcacttc ttcactgcag tactggctgt agttgcgggg cgagccgcac gcgtagttct
     2221 cgtagtagtt cgcgtccggc tcgtccaccc cgaggccggt caggttggcc ccgatctggt
     2281 actccccgcg ggtcgccatc gggtgccact gagcggtctc cacctgcttg agcgtggcct
     2341 cgatgcccac ctgcttcagc tcgttgatca cgaacgaggc gaggtccacg tacatcgcga
     2401 tggcccgcgt gctcatctcc acgcgcagcg ggttctgcgg ggtgacgccc gcctcggcga
     2461 ggagcttccg ggcgtgcgcc ttcatgtccg ccggcttgcc gtagccgggc agcgcggcga
     2521 ggtccttgtc gaggaggccc cagaccccgt aaggcttggg ctgctgcgaa gcccccggca
     2581 ccgccccgcc ctggtgcacc gccgagatca gggcccggcg gtcgaccgcg taggccaccg
     2641 cgaggcgcac cttcggattg tcgaagggcg gcttcttggt gttcaggatg atgttgtcgc
     2701 tcaccgcctg ggccaccggg gtgatcacga gaccgggcac cgccttcttg agctgctcgg
     2761 ccgcggtctt ggtggtctca cccgggaacg acacgtcgag ctggccggcc tggagcgcgg
     2821 cggtgcgcgt cccccgctcc ttgatcacca cgtagcggag gctgtcgagg tacggccgcc
     2881 ccttcatgaa gtagtcgggg ttcttcacgt agtcaacgaa ctcacccttg cgccattcct
     2941 tcaccttgaa cggcccagtg ccgatgcagc cggtgcgata cgtcgccggg ttcacgtgcg
     3001 cggcgtagac cgggctgtag ccggaggcga gcatcaccag gagcccgggc tgcgggcgct
     3061 tcaggtggaa gaccaccgtg taggggtcgg gggcctcgat cttctcgacg ttggtgtacc
     3121 aatctttacg agggttgatc cgtagcttgg tggtcgcttc gggcgcctcg cgcaccatgt
     3181 cgaaggtgaa cttcacgtcc ttcgaggtga acggctggcc gtcgtgccac ttgacgtctt
     3241 tccggaggaa aaacacgaga tttctatagt tgtcctgcca ggaccacttc tcggccagct
     3301 cgccgaccag attgtcgacg gtctcggccc gcttggtctg gtcgaagacg accaggttgt
     3361 tgaagcacgg catcgacggg aacgaggtcg cgatggtcga cgtctcgtgg accgcgaagc
     3421 cctgcgagag atcctcgcgc tgcatgaggt tcaggtgccc gcccgacttc ggcgcttggg
     3481 ccgacgacgg gaccgtcgtc gcgacgaccg cggccaggcc gagcgagatc gcgatggcga
     3541 aggtgcggga acgcttcatt gtgctcctct ccttgcgccg gccgagcggc cggatggtag
     3601 tcgtcgacag ggctggtgtg gggaaaccct cgcaaaatat acggcatggg cggtggtccg
     3661 acaacccttc cgcccaggcg catcctcacg cggcgcgtta ttggcggagg gcgtgcgtcc
     3721 ggaccgcgag cgcggtcagc cggagggggt cggtgacgat cgggacagag agcgcgcgag
     3781 ctccgctagt acgaggacgg gcgcccgcgc tcgcggacct cccggcgcgt gaggatctcc
     3841 tcgagcgcga tggtgcgggc tccgccctga cggcgcagct cgggccgggc caggatgggg
     3901 cgctcgcgaa tgcgggccgc gaggccgatc ttcaccgccc gctcggtgta caggcgctgc
     3961 agggcgtggc acatcagcgg gcgtccgcgg ttgtagccga actgccgctt gtactcgtcc
     4021 gcggtcatcc cgtggttgcg cagatgggtg ttggtgagct ggcgaaacgc gctcccgcag
     4081 acgagacagc ggatgaggtc gtgctctacc gcctgccgtg gatcggcgcg cagcaatgcc
     4141 agcggcgacc gtcgcggcag ggggcgcagg gcgatgcccc ggctcgcgcc gtcgtcgaga
     4201 ccgaggacgc gcccgggcaa gacaatggcc ccgggagtgg gcgccgggat cgagtggcca
     4261 tggagggcgg gcatgtcggg ctcctctccg gatcgagcga tggcgga
//
LOCUS       DATMCV010000019         2615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_599666, whole genome shotgun sequence.
ACCESSION   DATMCV010000019 DATMCV010000000
VERSION     DATMCV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599666"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="VNU03_00540"
     CDS             <1..1788
                     /locus_tag="VNU03_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP72959.1"
                     /translation="RRSIALKLTLTLVGFVAVSVLAAGLYLNRALEAFAVESLDTRLA
                     ALAAVLDGDARAALVAGGPAAQDFAYRVARPTGSRVTLIAPDGRVVGESDRSLAELSQ
                     LENHAARPEVRAALEGRQGRDLRRSTTVAAPLLYMAAPVRDGGRIVGVLRLALPLSAV
                     TGSYEAIHQVMLVGGAVALLIALGIGVFVAGRVTHPVVEMQSIARQMSGGNFLVRAPT
                     RSTDEIGTLGRSLNVMAGRLREKIQDLEQEQSKTTAILDAMVEGVIAVDGQEHVLLMN
                     ERARAMFGLGATRGEGKPFQEVIRNSDLHEIFRAVHAGPDPGPVRRELLVTGPVTRTL
                     GVNAVRLAMTGDQLGVVLVLHDVTALRQLERVRTEFVANVSHELRTPLTAIQGYLETL
                     LSGALEEPENARRFLEIVLRHSERLGRLLNDLTDLSNIELGKVTLRRESVRIGEVVAS
                     VVDIIAPRAARAGVAVSAQMPAELALLSADRDRLAQVLINLIDNAVKYTPDGGAVTVT
                     GRNLGPDRIEVAVADTGIGIPPADLPRITERFYRVDKARSRELGGTGLGLAIVKHLVM
                     AHGGELSIQSEQERGTTVRFTLPIEPSAA"
     gene            1898..>2615
                     /locus_tag="VNU03_00545"
     CDS             1898..>2615
                     /locus_tag="VNU03_00545"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HWP72960.1"
                     /translation="MNNLIAHDRERRESTARGRADLHVHTLYSDGGQSPEAVVRAAAG
                     RVDVVGITDHDRVGGALRARDYAREHPELGVDVVVGEEISTLNGHLIGLYLREAVPAG
                     LSARETIERIHAQDGLAVAAHPFHPLRGVARGHQPIGRLIPDLAFDAIEVVNNAGVFS
                     RLYDAWAALRNVEWMLPVTAGSDAHDVWYLGSACTTFPGRDAAAFRGALEAGRTRARV
                     EWSWTAAKMPRHVHIQSRSAI"
BASE COUNT          356 a          925 c          977 g          357 t
ORIGIN      
        1 cggcgcagta tcgcgctcaa gctcacgctg accctggtgg gcttcgtggc ggtgagcgtc
       61 ctcgcggccg gtctctacct gaaccgggct ctcgaggcct tcgcggtcga gtccctggac
      121 acccggctgg ccgcgctggc cgcggtgctc gacggcgacg cccgcgccgc actcgtcgcc
      181 gggggaccgg cggcccagga cttcgcgtac cgagtggcgc ggcccaccgg ctcgcgggtg
      241 accctgatcg cgcccgacgg gcgggtggtg ggcgagtcgg accggagcct ggccgagctg
      301 agccagctcg agaaccacgc ggctcggccg gaggtgcggg ccgccctcga gggccgccag
      361 ggccgcgacc tgcggcggag caccaccgtc gcggccccgc tcctctacat ggcggcgccg
      421 gtgcgcgacg gtgggcggat cgtcggcgtt ctccgcctcg ccctgccgct ctccgcggtg
      481 accgggtcgt acgaggcgat tcaccaggtg atgctggtgg gtggcgcggt ggcgctcctg
      541 atcgccctcg gcatcggcgt cttcgtggcc ggccgggtga cccacccggt ggtggagatg
      601 cagtcgatcg cccgccagat gagcggcggc aacttcctgg tccgggcgcc cacccgctcg
      661 accgacgaga tcggcacgct cggacggtcc ctcaacgtga tggcggggcg gctgcgcgag
      721 aagatccagg atctcgagca ggagcagtcc aagaccaccg cgatcctcga cgcgatggtc
      781 gagggcgtga tcgcggtgga cggccaggag cacgtcctgc tcatgaacga gcgggcccgc
      841 gccatgttcg gcctgggcgc gacccggggg gagggcaagc cgttccagga ggtgatccgc
      901 aactcagatc tccacgagat cttccgggcg gtgcacgcgg gcccggatcc ggggccggtc
      961 cgccgcgagc tcctggtgac cggcccggtg acgcgcaccc tgggggtgaa cgcggtgcgg
     1021 ctcgcgatga ccggcgacca gctcggggtg gtcctggtcc tccacgacgt gaccgcgctc
     1081 cgccagctcg agcgggtgcg gaccgagttc gtggccaacg tgagccacga actgcgcacg
     1141 ccgttgaccg cgatccaggg ctacctcgag acgctcctgt cgggcgccct cgaggagccg
     1201 gagaacgcgc gccgtttcct cgagatcgtg ctgcgccaca gcgagcggct cgggcggctg
     1261 ctcaacgacc tgaccgatct ctccaacatc gagctcggca aggtgacgct gcggcgcgag
     1321 tcggtgcgta tcggcgaggt ggtcgcctcg gtggtggaca tcatcgcgcc gcgcgccgcg
     1381 cgggcggggg tcgcggtgtc cgcccagatg ccggccgagc tggccctgct ctcggcggat
     1441 cgcgatcgcc tcgcccaggt gctgatcaac ctcatcgaca acgcggtgaa gtacacgccc
     1501 gacggcggag cggtgacggt gaccggtcgc aacctgggtc ccgaccgcat cgaggtggcg
     1561 gtggccgaca ccggcatcgg catccctccc gcggacctgc cccgcatcac cgagcgcttc
     1621 taccgggtcg acaaggcccg ctcgcgcgag ctggggggta ccggcctggg cctcgccatc
     1681 gtcaagcacc tggtcatggc ccacggcggc gagctctcga tccagagcga gcaggagcgg
     1741 gggaccaccg tccgcttcac cctgccgatc gagccctccg cggcctgatc gcggccacgg
     1801 cggcccgcgc gcgagcgcgt ggcccgacct ctccgtcaca caaacttcac gccgggagcg
     1861 gtatctcatc aatgcccgtc gtgcatgctg gatcgcgatg aacaacctca tcgcgcacga
     1921 tcgggagcgg agggaatcga cggcccgcgg ccgcgcggac ctgcacgtgc acacgctcta
     1981 cagcgacggc ggccagtcgc cggaggcggt ggtgcgggcc gccgcggggc gggtcgacgt
     2041 ggtgggcatc accgatcacg accgcgtggg cggcgcgctg cgcgcgcgcg actacgcgcg
     2101 cgagcacccg gagctggggg tggacgtggt ggtcggcgag gagatcagca cgctgaacgg
     2161 ccacctgatc ggcctctacc tgagagaggc ggtgccggcc gggctctccg cgcgggagac
     2221 catcgagcgc atccacgccc aggacgggct cgcggtggcg gcgcatccgt tccatcccct
     2281 gcgcggggtg gcccgcggcc accaaccgat cggccggctg atccccgacc tcgccttcga
     2341 cgcgatcgag gtggtcaaca acgcgggcgt gttctcccgc ctctacgacg cgtgggccgc
     2401 cctgcggaac gtggagtgga tgctgccggt gaccgcgggg agcgacgcgc acgatgtctg
     2461 gtacctcggc agcgcgtgca ccacgttccc gggacgggac gcggcggcgt tccgcggcgc
     2521 gctcgaggcg gggcggacgc gggctcgggt cgagtggtcg tggacggcgg cgaagatgcc
     2581 gcggcacgtg cacatccagt cacgcagcgc gatca
//
LOCUS       DATMCV010000020         5866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_400331, whole genome shotgun sequence.
ACCESSION   DATMCV010000020 DATMCV010000000
VERSION     DATMCV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400331"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="VNU03_00550"
     CDS             complement(<1..1544)
                     /locus_tag="VNU03_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWP72961.1"
                     /translation="MRLGVRGREALVVSLLTLLVVATATAIHLSQVSRVVVQEAVKQA
                     ELIAKQIYAQSGRALQRAPGADPLDALRDDPELRSFLDASVGYSPHLLYALISDATGT
                     VLLHTERRKEGQSAPERPSLEGLLKLDTLSRFSALYNAGRTYETALPVTLNDRFFGSI
                     RLGVSTTLLRREVTASLQQSLAVAAVALPLAWLAAMVLAQLTVRPIRAIVGQVDRIRR
                     GEPVEATAVVGGDEFQELSAQLSRLGRELQADRLSTLSEKAHLQQVVDQLEDGVIFLN
                     PERRILFFNRVAETVVGLSLEQTVGLPIHEVLDPTHPLLPFVDQVVSGATGIRNATVM
                     LPQDGREKEFLVSAFHMQDAHRVMGVAVLLKDLESIKTVQSLVSYSAKLAALGRLTSG
                     VAHEVKNPLNAMMIHVELLKERLEAADPDVRQSLEVIGSEIRRLDRVVQGFLKFMRPQ
                     ELTLKPVDLNSMLQSVGALLEAEGQSLGVRFILDLDAGLPSVSADEELLRQAFINIVQ
                     NAVQAMP"
     gene            complement(1548..2906)
                     /locus_tag="VNU03_00555"
     CDS             complement(1548..2906)
                     /locus_tag="VNU03_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP72962.1"
                     /translation="MALKILIVDDEEPARQGLSALLARWGYEVDEAGDGQEALAKAAA
                     GLPSGVLSDLVMPKMDGLELLRALKTDVPFASVILLTGQGSIDTAVTAMREGAYDYLT
                     KPVDVARLRLLIPKAAERGESLREVALLRRKLSQVWGMGRLVGTSHGMQEVYRLIEVA
                     APTPAAVLISGESGTGKELVARTLHELSPRAKGPFVAVNCAAIPETLLESEIFGHEKG
                     AFTGALERRPGCFELAHEGTIFLDEIAEMNPGTQAKFLRILQDSTVRRLGGKTEIKVD
                     VRVLAATNKDPVKAIQEGTFREDLYYRLNVVSLVMPPLRERRDDIPALVEAFLEEFNA
                     RYDKHIRSLDEGVLSGLIAQSWPGNVRELRNTLERAIIVCEGDTILPKHIPPKPSLRA
                     ADPAEGPDSVSFRVGTSLEDAEKAIIIKTLAANGNNKTRAADVLGISLKTLHNKLKTY
                     GT"
     gene            complement(2923..4254)
                     /locus_tag="VNU03_00560"
     CDS             complement(2923..4254)
                     /locus_tag="VNU03_00560"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWP72963.1"
                     /translation="MPNIVVVGAQWGDEGKGKIVDVLAPHVGVVVRYQGGNNAGHTVV
                     VGKEKFVLQSIPSGILHSGVRCVIGCGVVIDPASLIEEMESLVQRGVSLDGNLYISKN
                     AHLIMPYHPALDRASESQAGDRRIGTTGKGVGPAYADKAARIGIRMADLLDERLFREK
                     LEFNIKQKNRLLREIYDEQTFTVDGILGTYLRYAGWLAPYITDTALLLHRWIEAGRSV
                     LFEGAQGTMLDIDHGTYPFITSSSTTAGGASTGTGVPPTKLDGVLGISKAYCTRVGGG
                     PFMTEVSGEMADKLRARGNEYGAVTGRPRRCGWADAVALRYAARINGLDTIALTKLDV
                     LDGLETVRICVGYRYGGEVLTDFPEEERIWDEAEPVYEELSGWQTSTHGMRDYAELPS
                     KAREYVERLAELIGVGFSLVSTGAVRDETILMSDSVLTRWFPALRSALPAH"
     gene            complement(4247..5581)
                     /gene="hisZ"
                     /locus_tag="VNU03_00565"
     CDS             complement(4247..5581)
                     /gene="hisZ"
                     /locus_tag="VNU03_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWP72964.1"
                     /translation="MSLPRKNQPTQLPKGAKIYLPDEAARKRAVEERLFEVFRRWGYR
                     EVVTSAYEYFDVLSHGTDLELQERMFKMVDRESGRLLALRADVTPQIARIVATRMREE
                     PKPLRLHYVTNVFRYDEPHVGRYREFYQAGVELIGLPNPEGDAEMIAMTVEGLRTLGL
                     ERFQIDVGQADFFRGILEDLAVDPAAARELRDALGRKDLSALERLVGDLAAPAAVSEL
                     LLALPTLYGRGDVLERAERLVKNARSEAALANLAEVYRLLRAYGLADSVLLDLGEVRG
                     FDYYSGVHFEAYVTGLGAPLVGGGRYDQMLARFGYDCPATGFAFETGRALLAMESQGA
                     EPRLAGPDFFVIDFTADKNRALAISRRLRDAGAAVARDIISRPLAESLAYARGQRAVW
                     ALVIGADGTDPDHVAVVDLQTGADTPGERTVRLEDLLADPRPHFPGIGGGHA"
     gene            complement(5593..>5866)
                     /locus_tag="VNU03_00570"
     CDS             complement(5593..>5866)
                     /locus_tag="VNU03_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWP72965.1"
                     /translation="PGAETLAAVRTRALAGLAALRAAHQGQTICLVTHGITGRLLILE
                     ALGLGLDRLWSFQLSSTGISELEFRDDWTTLHRMNSLIHLADGAAA"
BASE COUNT          842 a         2079 c         2065 g          880 t
ORIGIN      
        1 ggcatggcct gcaccgcgtt ctgcacgatg ttgatgaagg cctgtcgcag cagctcctcg
       61 tcggcgctca ccgagggcag ccccgcgtcg aggtccagga tgaagcgcac cccgaggctc
      121 tgaccctccg cctccagcag cgcgcccacg ctctgcagca tcgagttgag gtccaccggc
      181 ttgagggtca gctcctgggg gcgcatgaac ttgaggaacc cctgcaccac gcggtccagc
      241 cgccggatct cgctgccgat gacctcgaga ctctgccgga cgtcggggtc ggccgcctcg
      301 agccgctcct tgagcagctc gacgtggatc atcatcgcgt tgagcgggtt cttcacctcg
      361 tgggcgaccc cggaggtgag ccgccccagc gcggcgagct tcgccgagta gctcacgagg
      421 gactgcacgg tcttgatcga ctccagatcc ttcagcagca ccgccacccc catcacccgg
      481 tgggcgtcct gcatgtggaa ggcggagacg aggaactcct tctcgcggcc gtcctggggg
      541 agcatcacgg tggcgttgcg gatcccggtg gcgccgctca ccacctggtc cacgaagggc
      601 agcagcggat gggtgggatc gagcacctcg tggatgggca gcccgaccgt ctgctcgagc
      661 gagaggccca ccaccgtctc cgcgacccgg ttgaagaaca ggatgcgccg ctccgggttc
      721 aggaagatca ccccgtcctc gagctgatcg accacctgct gcaggtgcgc cttctcgctg
      781 agcgtggaca ggcggtcggc ctgcagctcg cggccgaggc gcgagagctg ggcggagagc
      841 tcctggaact cgtcgccgcc caccaccgcg gtcgcctcca ccggctcgcc ccggcggatg
      901 cggtcgacct ggcccacgat ggcgcggatg ggccgtaccg tgagctgggc caggaccatc
      961 gcggcgagcc aggcgagcgg cagggccacc gcggccaccg cgaggctctg ctggagcgac
     1021 gcggtcacct cgcggcggag gagcgtggtg gagacgccga gccggatcga gccgaagaag
     1081 cggtcgttca gggtcaccgg cagcgcggtc tcgtaggtac gccccgcgtt gtagagcgcc
     1141 gagaagcggc tcagggtgtc gagcttgagg aggccctcga ggctcggccg ctcgggggcg
     1201 gactgccctt ccttgcgccg ctcggtgtgc agcaggacgg tgccggtggc gtccgagatg
     1261 agcgcgtaga ggagatgtgg cgagtagccc acgctcgcgt cgaggaagct ccgcagctcg
     1321 gggtcgtccc gcagcgcgtc cagcggatcg gcgccggggg cgcgctgcag cgcgcggccg
     1381 ctctgggcgt agatctgctt ggcgatcagc tccgcctgct tgaccgcctc ctgcaccacc
     1441 acgcggctca cctgcgagag atggatcgcg gtcgcggtgg cgaccaccag cagggtgagg
     1501 aggctgacga ccagcgcctc gcgcccgcgg actccgagcc gcatcgctta ggtgccgtag
     1561 gtcttgagct tgttgtgcag cgtcttcagg ctgatgccga gcacgtcggc ggcgcgggtc
     1621 ttgttgttgc cgttggccgc gagagtcttg atgatgatgg ccttctccgc gtcctccagg
     1681 ctcgtgccca cccggaagga cacgctgtcg ggaccctcgg ccgggtcggc cgcccgcagc
     1741 gagggcttcg gcgggatgtg cttgggcagg atcgtatcgc cctcgcagac gatgatggcc
     1801 cgctcgaggg tgttgcgcag ctcgcggacg ttgccgggcc acgactgggc gatgagcccg
     1861 ctcagcaccc cctcgtccag cgagcggatg tgcttgtcgt agcgggcgtt gaactcctcg
     1921 aggaaggcct cgaccagagc cgggatgtcg tcgcggcgct cgcgcagggg cggcatgacc
     1981 aggctcacga cgttcaggcg gtagtagagg tcttcccgga acgtcccctc ctggatcgcc
     2041 ttgaccggat ccttgttggt ggccgcgagc acccgcacgt cgaccttgat ctcggtcttg
     2101 ccgcccagcc ggcgcaccgt gctgtcctgc aggatgcgca ggaacttggc ctgggtcccc
     2161 gggttcatct ccgcgatctc gtcgaggaag atggtgccct cgtgggccag ctcgaagcag
     2221 cctggccgcc gctcgagcgc gccggtgaac gcccccttct cgtggccgaa gatctcactc
     2281 tcgaggaggg tctccgggat cgccgcgcag ttgaccgcca cgaagggccc cttggcccgc
     2341 ggggacagct cgtggagggt gcgcgccacc agctccttgc cggtaccgct ctcgccgctg
     2401 atcagcaccg cggccggagt cggcgcggcc acctcgatga ggcggtagac ctcctgcatg
     2461 ccgtggctcg tgcctaccag ccggcccatg ccccacacct ggctcagctt gcggcggagc
     2521 agggccacct cgcgcagcga ctcgccgcgc tcggcggcct tggggatgag caggcgcagc
     2581 cgggcgacgt ccaccggctt ggtcaggtag tcgtaggcgc cctcccgcat cgcggtcacc
     2641 gcggtgtcga tcgagccctg accggtgagc aggatgaccg acgcgaacgg cacgtcggtc
     2701 ttgagcgcgc gcagcagctc gagcccgtcc atcttgggca tcacgagatc cgagaggacc
     2761 ccggagggca ggccggcggc cgccttggcg agggcctcct gcccgtcgcc cgcctcgtcg
     2821 acctcgtagc cccagcgggc gaggagcgcg ctcaagccct ggcgggccgg ctcctcgtcg
     2881 tcgacgatca ggatcttgag ggccatgagg taaagccgcg ccctagtggg cgggcagcgc
     2941 gctccggagc gccgggaacc agcgggtcag gaccgagtcg ctcatcagga tcgtctcgtc
     3001 gcgcaccgcc ccggtggaga cgagcgagaa gccgaccccg atcagctccg cgagccgctc
     3061 cacgtactcg cgcgccttcg acggcagctc cgcgtagtcg cgcatgccgt gggtggacgt
     3121 ctgccagccg gacagctcct cgtagacggg ctcggcctcg tcccagatcc gctcctcctc
     3181 ggggaagtcg gtcagcacct cgcccccgta gcggtagccc acgcagatgc gcaccgtctc
     3241 caacccgtcg aggacatcga gtttcgtgag cgcgatggtg tcgagcccgt tgatgcgggc
     3301 cgcgtagcgc agggccaccg cgtcggccca gccgcagcgg cgcggccgcc cggtgaccgc
     3361 cccgtactcg ttgccgcggg cgcggagctt gtccgccatc tccccgctca cctcggtcat
     3421 gaacggcccg ccgcccaccc gcgtgcagta agccttcgag atgccgagca ccccgtcgag
     3481 cttggtgggc ggcaccccgg tgccggtgga ggcacccccc gcggtggtgc tcgaggaagt
     3541 gatgaacggg taggtcccat gatcgatgtc gagcatggtg ccctgggcgc cctcgaacag
     3601 gacggagcgc cccgcctcga tccagcggtg cagcagcagc gcggtgtcgg tgatgtaggg
     3661 cgcgagccag cccgcgtagc gcaggtaggt ccccaggatc ccgtcgacgg tgaacgtctg
     3721 ctcgtcgtag atctcgcgga gcagccggtt cttctgcttg atgttgaact cgagcttctc
     3781 gcggaagagc cgctcgtcga gcaggtcggc catgcggatg ccgatgcgcg ccgccttgtc
     3841 cgcgtaggcg ggacccaccc ccttgccggt ggtgccgatg cgacgatcgc cggcctgcga
     3901 ctcggaggcc cggtccagcg ccgggtggta gggcatgatc aggtgcgcgt tcttggagat
     3961 atagaggttg ccgtccaggg acaccccccg ctggaccagt gactccatct cctcgatcag
     4021 cgaggcgggg tcgatcacca ctccgcagcc gatcacgcag cggaccccgc tgtgcaggat
     4081 gccggagggg atggactgca gcacgaactt ctccttgccc accaccaccg tgtggcccgc
     4141 gttgttgccc ccctggtagc ggacgaccac gcccacgtgg ggggccagca cgtccacgat
     4201 cttgcccttc ccctcgtcgc cccactgcgc cccgaccacc acgatgttag gcatgaccgc
     4261 ctccgattcc cggaaaatgc ggacgcgggt ccgcgagcag atcctcgagc cgcaccgtcc
     4321 gctctcccgg cgtgtccgcg ccggtctgca ggtccacgac cgcgacgtgg tcgggatccg
     4381 tgccgtccgc cccgatgacc agcgcccaga ccgcgcgctg tccccgcgcg taggccagcg
     4441 actccgcgag cgggcggctg atgatgtcgc gcgccaccgc cgcccccgcg tcccgcagcc
     4501 gccgcgagat cgcgagcgcg cggttcttgt ccgcggtgaa gtcgatcacg aagaaatcgg
     4561 ggccggccag ccggggctcc gcgccctgcg actccatggc caggagcgcc cgtccggtct
     4621 cgaaggcgaa gccggtggcc gggcaatcgt agccgaagcg ggccagcatc tggtcgtagc
     4681 gcccgccccc caccagcggc gcccccaggc cggtcacata ggcctcgaag tgtacgcccg
     4741 aatagtaatc gaagccgcgg acttcgccca ggtccaggag gaccgagtcg gccaggccgt
     4801 aggcccgcag gaggcggtag acctcggcca ggttcgccag ggccgcctcc gagcgcgcgt
     4861 tcttgaccag ccgctcggcc cgctcgagca cgtcgccccg gccgtagagc gtgggcaggg
     4921 ccagcaggag ctcgctgacc gccgcggggg cggccaggtc gcccaccagg cgctcgaggg
     4981 ccgagaggtc cttgcggccg agggcgtcgc gcagctcgcg ggccgccgcg gggtcgaccg
     5041 ccaggtcctc caggatgccg cggaagaagt cggcctggcc cacgtcgatc tggaaccgct
     5101 cgagccccag cgtgcgcagc ccctcgaccg tcatcgcgat catctcggcg tcgccctcgg
     5161 ggttcggcag cccgatcagc tccacccccg cctggtagaa ctcgcggtag cgacccacgt
     5221 gcggctcgtc gtagcggaac acgttggtga cgtagtgcag ccgcaggggc ttgggctcct
     5281 cgcgcatccg ggtggcgacg atgcgcgcga tctgcggggt cacgtcggcg cgcagggcca
     5341 ggaggcggcc gctctctcgg tccaccatct tgaacatgcg ctcctgcagc tcgaggtcgg
     5401 tgccgtggga gaggacgtcg aagtactcgt acgcgctcgt caccacctcc cggtagcccc
     5461 agcgccggaa aacctcgaag aggcgctcct ccaccgcgcg cttgcgcgcg gcctcgtcgg
     5521 gcagatagat cttggcgccc ttgggcagct gggtcggctg attcttgcgg ggcaggctca
     5581 cggcaggggc cctcaggcgg cggcgccgtc ggccaggtgg atcagcgagt tcatgcggtg
     5641 cagcgtcgtc cagtcgtcgc ggaactcgag ctccgagatc ccggtcgacg agagctggaa
     5701 cgaccagagc cgatcgagcc ccaagccgag cgcctccagg atcaggagac gccccgtgat
     5761 cccgtgggtg acgagacaga tggtctggcc ctggtgggcg gcccgcagcg cggcgaggcc
     5821 cgcgagcgcc cgggtccgca ccgccgcgag ggtctcggcg ccgggc
//
LOCUS       DATMCV010000021         3776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_466562, whole genome shotgun sequence.
ACCESSION   DATMCV010000021 DATMCV010000000
VERSION     DATMCV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466562"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..753
                     /locus_tag="VNU03_00575"
     CDS             163..753
                     /locus_tag="VNU03_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72966.1"
                     /translation="MDSMQINPAGGGASPFRRPPRAPAVPAARPSPAFGPIPAAPLTA
                     PAPGPTAARPAPPSPVATDDKRERLLRLRRSGAFWFYWVAGLSVVNSLASLTGQQWRF
                     ILGLGITQLADALAVHSGRGIGAVAVMDAVIVGGFVLLGRFAERGRVWAFAVGAVIYA
                     LDGLIFVGLRDWIGVAFHVFVLVMTVRGLDAARRLA"
     gene            complement(787..1299)
                     /locus_tag="VNU03_00580"
     CDS             complement(787..1299)
                     /locus_tag="VNU03_00580"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWP72967.1"
                     /translation="MRRLLITLGWLVLATPAAAAPAAPAFTVTLLEGSGQHFDSRAQI
                     GKQVLVVRFQASWCKVCNEEAMGMERLYQKYRTRGVELVSVQVQDTEADARNFLKVNK
                     ATYPAGLDPRLKIANRFGFRGTPYTVVVNKRGEMVARIHGRADEARLSRILDPLVKAA
                     PARKPPARLQ"
     gene            complement(1353..1784)
                     /locus_tag="VNU03_00585"
     CDS             complement(1353..1784)
                     /locus_tag="VNU03_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP72968.1"
                     /translation="MPETTDDGSRATILVVDDEPDVRALIREVLTLNGYTVIDTGDPF
                     EARRIAERQPIQLLLTDVVMPIMNGIELAQRVEAASPTTKVLLMSGYVTAAVKTSGRP
                     LLSKPFRTNDLLAAIRQMLDSRSAFRRPAPPSAPRPGFGPV"
     gene            1989..>3776
                     /locus_tag="VNU03_00590"
     CDS             1989..>3776
                     /locus_tag="VNU03_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWP72969.1"
                     /translation="MNDLEHELRRRLEGDVRFDTYSRLLYATDASMYQMEPIGVVIPR
                     HAGDVQAAVEIANQQGVPLLPRGGGTSLTGQTVNRAVVMDFSRHMGGVLEVNREELWA
                     RVQPGLVQDELNHHVRPLGLLFGPDTSTSNRATLGGMIGNNSGGSHSIAYGLTVEHVI
                     EIQTILADGSRATFGTVTPAEFSAKMRAPGLEGRIYQEIDRIRTRYDAEIRTRYPRHW
                     RRVCGYNLDELVKDRPLNMARLIVGSEGTFLTVVEAKVRLVRKPKATALDVIHYHDMQ
                     EALESSQAILETGPYAVELTDKMILDLARNNIEQSQRMGFVQGDPAAILIVEYAGDTE
                     QEVKAKVADLEARRSRGRFGYASHIAADSAEQQSIWKLRKAGLGLLLGTRGESKPIAF
                     IEDTAVDPKHLPEFVPRFTEIMAKHGADAAYYGHCSVGCLHIRPLINLKTERGLEQVR
                     VMAEEIFDLVLSFGGAISSEHGDGRARSPFLERVFGPTMFRAFQETKAAFDPRGLMNP
                     GNIVGSPGVTEHLRYGPEYKTWEPKTLLDFSAQGGFAAAVEMCNGVGVCRKKLEGTMC
                     PSYMATRDEEHSTRGRANALRAVLSGKVPP"
BASE COUNT          535 a         1329 c         1336 g          576 t
ORIGIN      
        1 agcgggacct attggacctt ggtgctactg ggatgccgtc gtcccaagct tcgagccctc
       61 gatctggcac accgcttgct cgtttgtctt ccgcagcccg ttcgggcaat cgttcggtct
      121 cgatttggac agattgacac cggcggtgac ggaggagcgt acatggactc gatgcagatc
      181 aacccggccg gtggcggcgc cagccccttc cggcggccgc cccgggcgcc cgcggtgcct
      241 gccgctcgcc cgtcccccgc gttcggaccg atcccggccg cgccgctgac cgcgcccgcg
      301 ccgggcccga ccgccgctcg tcccgccccg ccgtccccgg tcgcgaccga cgacaagcgg
      361 gagcgactcc tccgtctgcg tcgcagcggg gctttctggt tctactgggt ggccgggctg
      421 tccgtcgtca actcgctggc ctcgctcacc gggcagcagt ggcgcttcat cctggggctc
      481 ggcatcaccc agctcgccga cgcgctcgcc gttcactccg gccgcgggat cggggcggtg
      541 gcggtgatgg acgcggtgat cgtcggcggc ttcgtgctgc tcggccggtt cgcggagcgg
      601 ggccgggtct gggctttcgc ggtgggcgcg gtgatctacg cgctcgacgg tctcatcttc
      661 gtgggcctgc gcgactggat cggggtcgcc ttccacgtct tcgtgctggt gatgacggtg
      721 cgcggcctcg acgccgcgcg ccgcctcgcc tgaccggtcc cgcgcccgcc ccgggcgcgc
      781 cgactcctac tgcagccgcg cgggaggctt gcgcgcgggc gcggccttca cgagcggatc
      841 gaggatgcgg gacaggcgcg cctcgtcggc gcggccgtgg atgcgggcca ccatctcgcc
      901 ccgcttgttg acgaccacgg tgtagggcgt gccgcggaag ccgaagcggt tggcgatctt
      961 gagccgcgga tcgaggcccg ccgggtaggt cgccttgttg accttgagga agttccgcgc
     1021 gtcggcctcg gtgtcctgca cctgcacgct caccagctcc acgccgcgcg tccgatactt
     1081 ctgatagagc cgctccatcc ccatcgcctc ctcgttgcag accttgcacc aggaggcctg
     1141 gaagcgcacc accagcacct gcttgccgat ctgggcgcgc gagtcgaagt gctgcccgga
     1201 gccttcgagg agcgtgaccg tgaacgcggg ggccgcgggg gccgccgcgg ccggggtcgc
     1261 gagcacgagc cagcccagcg tgatcaggag ccgtcgcacc gccccagtat aatccgcgcc
     1321 gggcgcggag gggcgaagcc cggcgggggc gctcagaccg gcccgaaccc cggccggggc
     1381 gcggacgggg gggcgggacg ccggaacgcg gagcgcgagt cgagcatctg gcggatcgcg
     1441 gccagcaggt cgttggtccg gaacggcttg gacaggagcg gccggccgga ggtcttcacc
     1501 gccgcggtga cgtagcccga catcaagagg accttcgtgg tcgggctcgc cgcctccacg
     1561 cgctgagcca gctcgatgcc gttcatgatc ggcatcacta cgtcggtgag gaggagctgg
     1621 atcggttgcc gctccgcgat ccgccgcgcc tcgaagggat cgccggtgtc gatcacggtg
     1681 tagccgttga gggtgaggac ctcgcggatg agcgcacgca cgtccggctc gtcgtcgacc
     1741 acgaggatcg tcgcgcggct gccgtcgtct gtcgtctcgg gcatcggtcc gtgtcctccg
     1801 gcgcctccac ggctgcaggg gaggtgccaa tggcggcccc attgtaaccc atcggtttcg
     1861 tggaaccgga cgatgtgcgc gcaccggcat cgtgtatttt ctccccgccg gagcccggtg
     1921 cttgcttccc gatcggcggg gccgcggcga agttgcgaac ctcgcggcct tggccgcata
     1981 atcccgccat gaacgatctc gagcacgagc tgcgtcgtcg tctcgagggc gacgtgcgct
     2041 tcgacaccta ctcgcgtctg ctctacgcaa ccgacgcctc catgtaccag atggagccga
     2101 tcggggtggt gatcccgcgg cacgcgggcg acgtgcaggc cgcggtcgag atcgccaacc
     2161 agcaaggggt gccgctcctg ccccggggcg gcgggacctc gctcaccggc cagaccgtga
     2221 atcgggcggt ggtgatggac ttctcgcgcc acatgggcgg cgtgctcgag gtcaaccgcg
     2281 aggagctgtg ggcgcgcgtg cagcccggcc tggtgcagga cgagctcaat caccacgtgc
     2341 ggccgctcgg gctgctcttc ggccccgata cctcgacgtc gaaccgggcc accctcggcg
     2401 gcatgatcgg caacaactcg ggcggctcgc actcgatcgc ctacgggctc acggtggagc
     2461 acgtcatcga gatccagacc atcctggccg acggcagccg cgccaccttc gggacggtga
     2521 cccccgccga gttctcggcc aagatgcgcg cgcccggcct ggagggccgc atctaccagg
     2581 agatcgaccg catccgcacc cggtacgacg cggagatccg cacgcgctat ccgcggcact
     2641 ggcggcgggt ctgcggctac aacctggacg agctggtgaa ggaccgcccg ctcaacatgg
     2701 cccgcctgat cgtgggctcg gaggggacct tcctcacggt ggtggaggcg aaggtgcgcc
     2761 tggtgcgaaa gcccaaggcc actgcgctcg acgtcatcca ctaccacgac atgcaggagg
     2821 cgctcgagtc ctcgcaggcg atcctcgaga ccgggcccta cgcggtcgag ctgaccgaca
     2881 agatgatcct cgacctggcc cgcaacaaca tcgagcagtc ccagcgcatg ggcttcgtgc
     2941 agggcgatcc agccgccatc ctcatcgtgg agtacgcggg cgacaccgag caggaggtga
     3001 aggccaaggt cgcggacctc gaggcccgcc ggagccgcgg ccgcttcggc tacgcctccc
     3061 acatcgcggc cgactcggcc gagcagcagt cgatctggaa gctgcggaag gccgggctgg
     3121 gcctgctcct ggggacgcgc ggggagtcca agcccatcgc gttcatcgag gacaccgcgg
     3181 tggacccgaa gcacctgccc gagttcgtgc cgcgcttcac cgagatcatg gccaagcacg
     3241 gggccgacgc ggcctactac ggccactgct cggtggggtg tctccacatc cgcccgctga
     3301 tcaacctcaa gaccgagcgc ggcctcgagc aggtgcgggt gatggcggag gagatcttcg
     3361 atctcgtcct ctccttcggg ggcgccatct cgagcgagca cggcgacggc cgcgcgcgga
     3421 gcccgttcct ggagcgggtg ttcggcccca ccatgttccg cgccttccag gagacgaagg
     3481 ccgccttcga cccgcgcggg ctcatgaacc ccggcaacat cgtggggagc cccggcgtca
     3541 ccgagcacct gcgctacggc cccgagtaca agacctggga gcccaagacc ctgctggact
     3601 tctccgccca gggcggcttc gccgcggcgg tggagatgtg caacggcgtg ggcgtgtgcc
     3661 ggaagaagct cgaagggacc atgtgcccgt cgtacatggc gacgcgggac gaggagcact
     3721 cgacgcgcgg acgggcgaat gcgctccggg ccgtgctctc gggcaaggtg ccgccg
//
LOCUS       DATMCV010000022         5551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_68228, whole genome shotgun sequence.
ACCESSION   DATMCV010000022 DATMCV010000000
VERSION     DATMCV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68228"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1529)
                     /locus_tag="VNU03_00595"
     CDS             complement(174..1529)
                     /locus_tag="VNU03_00595"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HWP72970.1"
                     /translation="MAQDLPGYLDLIKRSRPDDLLVVSKEMDPAFEITALVVKLEREA
                     RRRPVVICERVKGTPFPVLTNLHASRSRLALAMGAAPDEMLATYLRAMDKPIAPRVVA
                     TGPCKDVVLTGDRVNLYDLPQILHHEGDAGAYLTSAISFAKDPTSETWNCAYNRLMIQ
                     GRDRTSIHLTLGKHLWEFQRIAESLGRPLPVAFAIGVHPAIALGSLAIGSIDEDERAI
                     MGALLREPLELVKCETSDVLAPAHAEMIIEAEIQPGARTPEGPFGEFTGYSLGERQRE
                     VVQVKAITHRRGAYFQDITVAHLDHMLLSTIPMEANLYRAVRAMVPSVKAVRVPAPFT
                     CYVSLEQRLIGQAKNAILAVLGADLYMKRVVVVDQDVNIFDDRQVTWAIATRCQPDRD
                     ITIITHARGSDLDPSTKEDGYSAKWGVDATAKPSLAGYTPRHRVPPEVWKRLNLKDYT
                     E"
     gene            complement(1569..2324)
                     /locus_tag="VNU03_00600"
     CDS             complement(1569..2324)
                     /locus_tag="VNU03_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP72971.1"
                     /translation="MTASHWRQLASVAGLLLAWQILVQAGRINPLFLPAPLSVLGAMW
                     EMTRSGELPWAVLVSLNRVVQGFVYGSLAGVLLGLLAGAIRWVEDLLDPWVAAVYPIP
                     KSALFPLFMLWFGLGNPSKIVTIAVGVLFLVLVNTMAGVHAINPVLLKAARDLGASRA
                     QIFVKVILPGALPSIFTGLRLGAGMALILVFITEIEATKAGLGFLLWEAFQLMDTRHV
                     FVGVVTFGLLGVATTWLLQWLERVTCPWIQRSN"
     gene            complement(2321..3085)
                     /locus_tag="VNU03_00605"
     CDS             complement(2321..3085)
                     /locus_tag="VNU03_00605"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP72972.1"
                     /translation="MLLALRDVGKAWHIARTGERVLALQAITLDVAPGEFLILLGPSG
                     CGKSTLLQIMAGLEAPSSGAIEFPTPTGGGKLTSMVFQDYALFPWRTVLANVAFGPEV
                     RGVPRREREERARRLIELVNLRGAETRYPHELSGGMRQRVALARALANDPQALLFDEP
                     LAALDSQTRRVMQDELLRIWAETGKTFVYVTHSLQEAVLLGSRIVLMTAGPGRIKRVE
                     EVTLPRPRHLTGRAEAALLDRLDALLGEEVQRAMAS"
     gene            complement(3107..4087)
                     /locus_tag="VNU03_00610"
     CDS             complement(3107..4087)
                     /locus_tag="VNU03_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP72973.1"
                     /translation="MARPTRRAAIVLALLLAITLPAAAAETVKLGDLPAISNAGIYIA
                     IEKGYFQEHGVAVDIERFASAGKMTAPLATGQIDVSVGSPSAGLFNSIAAGMDFKIVA
                     DKGQVRPGYNFTPLIVRKDLVDSGKVKTLRDLKGLKVANGAKGINFDFFLAKMMEHVG
                     LTYDALDVVYMSYPDGIKALASKAVDAAFAPEPWGAQAEKEKIGTRLFLTEQVPALAT
                     FQVAAIMYSGKFIKERPKVARAFMQAYVQGLKLYNGKGGLKDAEVAGILAKHLKLPVE
                     TIQATLPVYLDPTGRPRVQDLAAFQDWFHQMGWVKEKVAMDRVVDLSFLE"
     gene            complement(4218..4880)
                     /gene="tenA"
                     /locus_tag="VNU03_00615"
     CDS             complement(4218..4880)
                     /gene="tenA"
                     /locus_tag="VNU03_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HWP72974.1"
                     /translation="MSATGALWQAIEPIYAAILAHPFVRGLTDGSLPREAFRFYAVQD
                     TLYLRDFARALSVAAARAPREEWIIMFNEHAAGALKVEMTLHESFFKDFGLSPEEVAA
                     TPLAPTNAAYTSYLLAVAYGRPFHEAVAALLPCYWIYWEVGKTLERAGSRDPLYGRWI
                     GTYASEEFGAVVRAVLACTDAFAAALGPDERRAMHRHFVTTSRYEWMFWDMGWRRETW
                     PV"
     gene            complement(4877..>5551)
                     /locus_tag="VNU03_00620"
     CDS             complement(4877..>5551)
                     /locus_tag="VNU03_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72975.1"
                     /translation="GPIPRDRIHLYLLGGLSVAMSGLFLWYPLRWMRRHGLGRTGALP
                     TAVYFGALGVGFIVVEVVLISKFVVLIGFPIYAMAAVLCTLLIAAGVGSRLSGWLRRR
                     HPDWAIGVFPTVALLIGVVVAAFPFARDLALGLGQPARIALSAALLLPLGVALGMPFP
                     LGIAALSRLAPRLVPWAWGVNGFMTVVGSLIAALLSIRWGFDVTLLAGATVYLVAMGA
                     WRALDA"
BASE COUNT          892 a         1876 c         2018 g          765 t
ORIGIN      
        1 atgatctcgc agcaactccg gcgcatcgag gccagcctcg cggacgacct cggccggcgt
       61 cagatcatgg agcgtgatct cgccgagctc aaggagcgcg tcgccgtgct ccagggccgc
      121 atcaacgagc tcgagcgccg acttggcgcc tgagcccggc cgccgtccgc cctctactcc
      181 gtgtagtcct tcaggttgag gcgcttccac acctcgggcg gcacccggtg gcgcggggtg
      241 tagccggcca gcgacggctt ggcggtcgcg tcgacccccc acttggcgga gtagccgtcc
      301 tccttggtgg agggatcgag gtcggagccg cgcgcgtgcg tgatgatcgt gatgtcgcga
      361 tcgggctggc agcgggtggc gatggcccag gtcacctggc ggtcgtcgaa gatgttcacg
      421 tcctggtcga cgacgaccac gcgcttcatg tagaggtcgg cgccgagcac ggccaggatc
      481 gcgttcttgg cctggccgat gaggcgctgc tcgagcgaga cgtagcaggt gaagggcgcg
      541 ggcacccgca ccgccttcac cgacggcacc atggcccgga ccgcccggta gaggttggcc
      601 tccatcggga tggtcgagag cagcatgtgg tcgaggtgcg ccaccgtgat gtcctggaag
      661 taggcgcccc gccggtgggt gatcgccttg acctggacca cctcgcgctg ccgctcgccc
      721 aggctgtagc cggtgaactc gccgaacggc ccctcggggg tccgcgcgcc gggctggatc
      781 tccgcctcga tgatcatctc ggcgtgcgcg ggggccagca cgtcggaggt ctcgcacttc
      841 accagctcga gcggctcgcg caggagggcg cccatgatcg cgcgctcgtc ctcgtcgatg
      901 gagccgatgg cgagcgagcc gagcgcgatc gcggggtgca ccccgatggc gaaggccacc
      961 gggagcggcc ggccgagcga ctccgcgatg cgctggaact cccagaggtg cttgccgagc
     1021 gtcaggtgga tcgacgtgcg gtcgcgcccc tggatcatca gccggttgta ggcgcagttc
     1081 cacgtctcgc tggtcgggtc cttggcgaac gagatcgcgg aggtgaggta ggcgcccgcg
     1141 tcgccctcgt gatggaggat ctggggcagg tcgtacagat tgacgcggtc gccggtcagc
     1201 accacgtcct tgcacgggcc ggtcgcgacc acccggggcg cgatgggctt gtccatcgcg
     1261 cgcaggtagg tggccagcat ctcgtcgggc gcggcgccca tggccagggc caggcgcgag
     1321 cggctcgcgt gcaggttggt cagcaccgga aagggggtgc ccttcacccg ctcgcagatc
     1381 accaccgggc gccggcgggc ctcgcgctcg agcttcacca ccagcgcggt gatctcgaag
     1441 gcggggtcca tctccttcga gacgacgagc agatcgtcgg gccggctgcg cttgatcagg
     1501 tcgagataac cggggaggtc ctgtgccatg tcgcgcctct ctgagcttcg tctagttcga
     1561 gcgctggatc agttcgagcg ttgaatccag gggcaggtga cccgctccag ccactgtaac
     1621 agccacgtgg tcgccacgcc cagcaggccg aacgttacca cacccacgaa cacgtgccgc
     1681 gtatccatga gctggaaggc ctcccacagg agaaagccga ggcccgcctt ggtggcctcg
     1741 atctcggtga tgaagaccag gatgagcgcc atgcccgcgc cgaggcgcag gcccgtgaag
     1801 atgctgggca gcgcgccggg caggatcacc ttgacgaaga tctgcgcccg gctcgcgccc
     1861 aggtcgcgcg cggccttcag cagcaccggg ttgatcgcgt gcaccccggc catggtgttg
     1921 accagcacca ggaagagcac gcccaccgcg atggtcacga tcttggacgg gttgcccagg
     1981 ccgaaccaca gcatgaagag cgggaagagc gccgacttgg ggatcgggta gaccgcggcg
     2041 acccagggat cgaggaggtc ctccacccag cggatcgcgc ccgcgagcag gcccaggagc
     2101 acgccggcga gcgagccgta gacgaacccc tgcaccaccc ggttcaggct caccagcacc
     2161 gcccacggca gctcgccgga gcgggtcatc tcccacatcg cgccgagcac cgagagcggg
     2221 gcgggaagga agagcgggtt gatgcgaccg gcctgcacca ggatctgcca ggcgagcagg
     2281 aggccggcca ccgaggcgag ctgccgccag tgcgacgcgg tcacgaggcc atcgcgcgct
     2341 gcacctcttc gccgaggagc gcgtcgaggc gatcgagcag cgcggcctcc gcgcgcccgg
     2401 tgaggtggcg gggccgcggc agcgtgacct cctccacccg cttgatgcgc ccgggcccgg
     2461 cggtcatcag gacgatgcga ctgccgagca gcaccgcctc ctgcaggctg tgggtcacgt
     2521 agacgaacgt cttgccggtc tcggcccaga tgcgcagcag ctcgtcctgc atcacccggc
     2581 gcgtctgcga gtcgagcgcg gcgagcggct cgtcgaagag gagcgcctgg gggtcgttgg
     2641 cgagcgcgcg ggccagggcc acccgctggc gcatgccgcc ggagagctcg tgcgggtagc
     2701 gggtctcggc cccgcgcagg ttgaccagct cgatcagccg ccgcgcgcgc tcctcgcgct
     2761 cgcgccgcgg caccccgcgc acctcggggc cgaaggccac gttggcgagc acggtgcgcc
     2821 aggggaagag cgcgtagtcc tggaacacca tgctggtgag cttgccgccg ccggtcgggg
     2881 tcgggaactc gatcgcgccc gacgacggcg cctcgagacc ggccatgatc tggagcagcg
     2941 tcgatttgcc gcagcccgac ggcccgagca ggatcaggaa ttcgcccggc gcgacgtcga
     3001 gggtgatggc ttgaagcgca aggacgcgct cgccggtgcg cgcgatgtgc caggccttgc
     3061 caacgtcacg gagggcgagc agcacggcgg gcgcgcgctt cccgggctat tcgaggaagg
     3121 agagatcgac cacccggtcc atcgccacct tctccttcac ccagcccatc tggtggaacc
     3181 agtcctggaa ggcggcgaga tcctgcacgc gcggccggcc ggtcggatcg agatagacgg
     3241 gcagggtggc ctggatggtc tcgacgggca gcttgaggtg cttggccagg atgcccgcga
     3301 cctcggcgtc cttgagcccg cctttgccat tgtagagctt gagcccctgc acgtaggcct
     3361 gcatgaacgc gcgggcgacc ttcggccgct ccttgatgaa cttgccgctg tacatgatgg
     3421 cggccacctg gaaggtggcg agcgcgggca cctgctcggt caggaagaga cgcgtgccga
     3481 tcttctcctt ctcggcctgg gcgccccacg gctcgggcgc gaaggcggcg tcgaccgcct
     3541 tgctcgcgag cgccttgatc ccgtcgggat aggacatgta gaccacgtcg agggcgtcat
     3601 aggtcagccc cacgtgctcc atcatcttgg cgaggaagaa gtcgaagttg atgcccttgg
     3661 ccccgttggc gaccttgagg cccttcaggt cccggagcgt cttcaccttg ccggagtcga
     3721 cgagatcctt gcggacgatg agcggggtga agttgtagcc gggacggacc tggcccttgt
     3781 cggccacgat cttgaagtcc atgccggccg cgatggagtt gaacaggccg gcggacggcg
     3841 agcccaccga cacgtcgatc tgcccggtgg cgagcggcgc ggtcatcttg cccgcggagg
     3901 cgaagcgctc gatgtcgacc gcgacgccgt gctcctggaa gtagcccttc tcgatcgcga
     3961 tgtagatgcc cgcgttggag atcgcgggca gatcgccgag cttgaccgtc tcggcggcgg
     4021 ccgccggcag cgtgatcgcc aggaggaggg ccaggacaat tgcggcgcgg cgggtcggtc
     4081 gggccatcgg atatgcctcc ggaggagcga tggtggggat gcgcgcgtag tctagcacag
     4141 cgcctcgccg tgctcccctc accgcccctc accctgctcc cctcaccctg ccctctcccc
     4201 ggaggggaga gggagagtta gacgggccag gtctcgcggc gccagcccat gtcccagaac
     4261 atccactcgt agcggctggt ggtcacgaag tggcgatgca tcgcccgtcg ctcgtccggg
     4321 ccgagcgcgg cggcgaaggc gtcggtgcag gcgagcaccg cgcgcaccac cgcgccgaac
     4381 tcctcggagg cgtaggtgcc gatccagcgc ccgtagagcg gatcgcgcga gcccgcgcgc
     4441 tcgagagtct tgccgacctc ccagtagatc cagtagcagg ggagcagcgc ggcgaccgcc
     4501 tcgtggaacg ggcggccgta ggccaccgcg aggaggtagg aggtgtaggc ggcattggtg
     4561 ggcgcgagcg gggtggccgc gacttcttcc ggggagaggc cgaaatcctt gaagaaggac
     4621 tcgtgcagcg tcatctccac cttgagcgcg cccgccgcgt gctcgttgaa catgatgatc
     4681 cactcctcgc ggggcgcgcg ggccgcggcc accgagaggg cacgggcgaa gtcccgcagg
     4741 tagagcgtgt cctgcaccgc gtagaaacgg aaggcctcgc gcgggagcga gccgtcggtg
     4801 agcccgcgca cgaacggatg cgcgaggatg gccgcgtaga tcggctcgat cgcctgccag
     4861 agcgcgccgg tcgcgctcat gcgtcgagtg cccgccaggc ccccatcgcc accaggtaca
     4921 cggtcgcccc cgcgagcagc gtcacgtcga agccccagcg gatcgagagc agcgcggcga
     4981 tcagcgagcc caccacggtc atgaagccgt tgacccccca cgcccacggc accaggcgcg
     5041 gggccagccg gctgagcgcg gcgatgccga gcgggaacgg catgccgagg gcgacgccca
     5101 gcggcagcag cagggcggcg gagagcgcga tgcgcgcggg ctggcccaaa ccgagcgcga
     5161 ggtcccgcgc gaaggggaag gcggccacca ccacgccaat caggagcgcc acggtgggga
     5221 acaccccgat cgcccagtcg gggtggcggc gccggagcca gccggagagg cggctgccca
     5281 ccccggcggc gatgagcagc gtgcagagca ccgcggccat cgcgtagatc gggaagccga
     5341 tcagcaccac gaacttcgag atcagcacga cctcgaccac gatgaagccg acgccgagag
     5401 ccccgaagta caccgcggtg ggcagcgcgc ccgtccggcc gagcccgtgg cggcgcatcc
     5461 agcggagcgg gtaccagaga aagaggcccg acatcgccac cgagagcccg ccgaggagat
     5521 agaggtggat gcggtcgcgg ggaatcgggc c
//
LOCUS       DATMCV010000023         3373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_932010, whole genome shotgun sequence.
ACCESSION   DATMCV010000023 DATMCV010000000
VERSION     DATMCV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932010"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /gene="urtC"
                     /locus_tag="VNU03_00625"
     CDS             <1..945
                     /gene="urtC"
                     /locus_tag="VNU03_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HWP72976.1"
                     /translation="LGAYAMGMHLMLEIGTRSVYGNVLPDFMVWNRVTELPLFWKPFY
                     SVPFTLLAMVVVPALFAFVFGYFTFRSRIRGVYFSIITQALALSTWLMFNRNAMNLGG
                     TNGLSGFKSIFGFTLNSAATQRGLYVATALCLCGAFLVCRYITRSPAGRVLIAIRDSE
                     TRVLFSGYSPAAFKLFVFVVSAALAGVAGALYAPQVGIITPAKIGVLPSIEMIIWVAV
                     GGRGTLLGPVVGALGVNWLQSLLTTHYPDLWLLVLGGLFVAVVLFFPDGVVGTGQKLV
                     ARLGPRGGAGARFRTRPEEAKGSEGTKTPTGVSHMEAR"
     gene            953..1747
                     /gene="urtD"
                     /locus_tag="VNU03_00630"
     CDS             953..1747
                     /gene="urtD"
                     /locus_tag="VNU03_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HWP72977.1"
                     /translation="MNFTAAAAEGSIIYLENVTVDFDGFKALHDLNFFMDYRELRVVI
                     GPNGAGKTTLLDVISGKVQPVAGRVIFGKHTDLTGRRENEIASLGIGRKFQTPSIFAN
                     LSVRENLELSLARPSKGVVATLRARLDGGQRERVEETLGTIGLGGRGGERAGGLSHGE
                     KQWLEIGMVIVQQAELLLVDEPVAGMTDEETARTAELLQSVARDRAVLVIEHDMEFVR
                     SLARKVTVLHEGSVLCEGSVDEIQQDERVREVYLGRSRSRTVGTHA"
     gene            1740..2441
                     /gene="urtE"
                     /locus_tag="VNU03_00635"
     CDS             1740..2441
                     /gene="urtE"
                     /locus_tag="VNU03_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HWP72978.1"
                     /translation="MLEVRGLNVAYGESQMLWDVALDVPAGSVVCLMGRNGVGKTTLL
                     KSIMGLLPVRSGRVAFDGSDLGGRRPEERAACGIGYVPQGREIFPNLTVQENLRVGVL
                     GTRRGSNGGGDPLEHVFELFPKLKVLLGRKGGVLSGGEQQQLAIARVLLAKPKLLLMD
                     EPTEGIQPSVIDQIEEAIERIRDQGISVLLVEQYLEFAWRLASAYAIMRKGTIVSRGS
                     TKDLREDMVRQHLTV"
     gene            2452..2754
                     /locus_tag="VNU03_00640"
     CDS             2452..2754
                     /locus_tag="VNU03_00640"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HWP72979.1"
                     /translation="MHLTPREQDKLLIFTASELARRRRARGLKLNYPEALALITAEVL
                     EGIRDGRTVSDLMAFGITILTRADVMEGVPEMLEEVQVEGTFPDGTKLVTIHHPIR"
     gene            2758..3129
                     /gene="ureB"
                     /locus_tag="VNU03_00645"
     CDS             2758..3129
                     /gene="ureB"
                     /locus_tag="VNU03_00645"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HWP72980.1"
                     /translation="MIPGEYLLGEGDIEANVGRRTLQVTVANTGDRPIQVGSHFHFFE
                     ANKALRFDRGRSFGMRLNVPAGTAVRFEPGDEKVVTLVELAGAREVYGLNALTDGPAN
                     EGRRAESVRRAAEGGYLGAGA"
     gene            3126..>3373
                     /gene="ureC"
                     /locus_tag="VNU03_00650"
     CDS             3126..>3373
                     /gene="ureC"
                     /locus_tag="VNU03_00650"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758739.1"
                     /note="ureases catalyze the hydrolysis of urea into
                     ammonia and carbon dioxide; in Helicobacter pylori the
                     ammonia released plays a key role in bacterial survival by
                     neutralizing acids when colonizing the gastric mucosa; the
                     holoenzyme is composed of 3 ureC (alpha) and 3 ureAB
                     (gamma/beta) subunits; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWP72981.1"
                     /translation="MSLRLTRRQYADLYGPTVGDRVRLADTDLLIEVERDFTSYGDEV
                     KFGGGKVIRDGMGQSARATAGPEVLDLVITNALILDHWG"
BASE COUNT          495 a         1109 c         1237 g          532 t
ORIGIN      
        1 ctcggggcct acgcgatggg catgcacctg atgctcgaga tcggcacccg cagcgtctac
       61 ggcaacgtgc tcccggattt catggtgtgg aaccgggtga ccgagctgcc cctcttctgg
      121 aagccgttct acagcgtgcc cttcaccctg ctcgcgatgg tggtggtgcc cgcgctcttc
      181 gcgttcgtgt tcggctactt cacgttccgg agccgcatcc gcggcgtcta cttctcgatc
      241 atcacccagg ccctcgcgct gagcacgtgg ctgatgttca accgcaacgc gatgaatctg
      301 ggcggcacca acggcctgag cggcttcaag tcgatcttcg gtttcaccct gaacagcgcc
      361 gccacccagc ggggtctcta cgtggccacc gcgctctgcc tctgtggggc cttcctggtg
      421 tgccgctaca tcacccgctc gcccgcgggc cgggtcctga tcgccattcg tgacagcgag
      481 acccgcgtgc tcttctcggg ctactcgccg gccgcgttca agctcttcgt gttcgtggtc
      541 tccgcggccc tcgcgggcgt cgcgggcgcc ctctacgcgc cccaggtcgg catcatcacc
      601 ccggccaaga tcggggtgct gccgtcgatc gagatgatca tctgggtggc ggtcgggggc
      661 cgggggacgc tcctggggcc ggtggtgggg gcgctcgggg tgaactggct gcagagcctc
      721 ctcaccacgc actaccccga tctctggctg ctcgtgctgg gcggtctctt cgtcgcggtg
      781 gtgctcttct tcccggacgg ggtggtgggc accggtcaga agctggtggc gcgcctggga
      841 ccgcgcggcg gggcgggtgc gcggttccgc acgcgccccg aggaggcgaa ggggtccgag
      901 gggaccaaga cgccgaccgg ggtctcccac atggaagcgc gctgagcggg gcatgaactt
      961 caccgccgcg gcggccgagg gctcgatcat ctacctggag aacgtgaccg tcgacttcga
     1021 cggcttcaag gccctgcacg acctcaactt cttcatggac taccgcgagc tgcgggtggt
     1081 gatcgggccg aacggagcgg gcaagaccac gctgctcgac gtgatctccg gcaaggtcca
     1141 gccggtggcg gggcgggtga tcttcggcaa gcacaccgac ctgactgggc ggcgtgagaa
     1201 cgagatcgcc tcgctcggga tcggccgcaa gttccagact ccgtcgatct tcgccaacct
     1261 gagcgtgcgc gagaatctcg agctctcgct cgcccgcccg agcaaggggg tggtggcgac
     1321 cctgcgggcg cgtctcgacg gtgggcagcg ggagcgcgtc gaggagacgc tcgggacgat
     1381 cgggctcggc ggccggggcg gggagcgggc cggcggcctc tcccacggcg agaagcagtg
     1441 gctcgagatc ggcatggtca tcgtgcagca ggccgagctg ctcctggtgg acgagccggt
     1501 ggccgggatg accgacgagg agaccgcccg gaccgcggag ctgctccagt cggtcgcgcg
     1561 ggaccgggcg gtgctggtga tcgagcacga catggagttc gtccggagcc tggctcgcaa
     1621 ggtgacggtg ctccacgagg gcagcgtcct ctgcgagggg tcggtcgacg agatccagca
     1681 ggacgagcgg gtgcgcgagg tctacctcgg ccggagccgg tcgaggacgg tgggcacgca
     1741 tgcttgaggt gcggggcctc aacgtggcct acggcgagag ccagatgctc tgggacgtcg
     1801 cgctcgacgt gcccgcgggc agcgtggtct gtttgatggg ccgcaacggg gtcggcaaga
     1861 ccaccctgct caagtcgatc atggggctcc tgcccgtccg atccggccgc gtcgccttcg
     1921 acggcagcga cctcggaggg cgccgcccgg aggagcgggc cgcttgcggc atcggctacg
     1981 tgccccaggg ccgcgagatc tttccgaacc tcaccgtgca ggagaacctg cgcgtggggg
     2041 tgctcggcac ccggcgcggg agcaacgggg gcggggaccc gctcgagcac gtgttcgagc
     2101 tgtttcccaa gctgaaggtg ctgctggggc gcaagggcgg cgtgctgtcg ggaggcgagc
     2161 agcagcagct cgcgatcgcc cgcgtgctcc tggcgaagcc caagctgctc ctgatggacg
     2221 agcccaccga ggggatccag ccgtcggtga tcgaccagat cgaggaggcc atcgagcgga
     2281 tccgggacca gggcatctcg gtgctgctgg tcgagcagta tctggagttc gcctggcgac
     2341 tggcttccgc ctacgcgatc atgcgcaagg gcaccatcgt gtcccggggc tcgaccaagg
     2401 acctgcgcga agacatggtc aggcagcacc tgacggttta aactcccgcc catgcacctg
     2461 accccccgcg agcaggacaa gctcctcatc ttcaccgcgt ccgagctggc ccgccggcgc
     2521 cgcgcgcggg gtctcaagct gaactacccc gaggcgctgg ccctgatcac cgcggaggtc
     2581 ctcgagggga tccgcgacgg ccggacggtg tcggacctca tggccttcgg catcacgatc
     2641 ctcacccgcg ccgacgtgat ggagggcgtg cccgagatgc tcgaggaggt ccaggtggag
     2701 ggcacgttcc cggacgggac caagctggtg acgattcacc atccgatccg gtgacgcatg
     2761 attcccggtg agtacctgct cggagaggga gatatcgagg ccaacgtggg gcggcggacg
     2821 cttcaggtga cggtcgcgaa caccggggac cggcccatcc aggtggggtc gcacttccac
     2881 ttcttcgagg cgaacaaggc gctgcgcttc gaccggggcc gctcgttcgg gatgcggctc
     2941 aacgtgccgg ccgggaccgc ggtgcgcttc gagcccggcg acgagaaggt ggtgaccctg
     3001 gtcgagctgg cgggagcgcg cgaggtctac gggctcaacg cgctcaccga cgggccggcg
     3061 aacgagggcc ggcgggccga gagcgtccgg cgggcggccg agggcgggta cctgggagcc
     3121 ggcgcgtgag cctccggctg acgcggcggc agtacgccga tctctacggg cccaccgtcg
     3181 gcgaccgggt gcgcctggcc gacaccgatc tgctcatcga ggtggagcgt gacttcacct
     3241 cctacggcga cgaggtgaag ttcggcggcg gcaaggtgat ccgcgacggc atgggccagt
     3301 cggcccgcgc caccgccggg ccggaggtcc tcgacctggt gatcaccaac gcgctcattc
     3361 tcgatcactg ggg
//
LOCUS       DATMCV010000024         4893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_932195, whole genome shotgun sequence.
ACCESSION   DATMCV010000024 DATMCV010000000
VERSION     DATMCV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932195"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VNU03_00655"
     CDS             complement(<1..406)
                     /locus_tag="VNU03_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72982.1"
                     /translation="MNLLENGLLRWVRGQPIALSSAGFAGPIILFLTGLAAATAAGRG
                     GIGLTPLSYSYAHETLLMASGRMRDALTQYVLWPPGYSLIAVPIVWLGVSPIRATWLI
                     SVAAFGMMVCLTFLLGRRLTSTTGGILAALLVL"
     gene            607..1743
                     /locus_tag="VNU03_00660"
     CDS             607..1743
                     /locus_tag="VNU03_00660"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWP72983.1"
                     /translation="MQSSPGSPLLSHIAALDGLRGLAILSVMFFHATLSRANAPRPSG
                     GFLGVDVFFVLSGFLITAVLLKEWDQKGSIALRSFYARRALRLLPALFVLMAVILLVP
                     GVFYSSQRPWRDAMVAAAYATNWVRAFGGDIGFFGHTWSLTIEEQFYVIWPLLLVGLL
                     RLRVRRRVILLLVLVAIAVPTVLRVDLWEGPMSVKRLYYGLDTRFDSLLLGCLVAFLA
                     SWNVIPRSERALKMIRLAAAAAAVVLVVLIANATEESQLTYFGLGVIACASVAVLLVH
                     LIYCPSRLSDLILANRPMVWVGRISYGLYLWHDPIFFGMLNPTRMGKVGVTGVGLMAV
                     RFVAAFVAATVSFYLLERPLLRIKDHLARPGAEPAGSALVEGPR"
     gene            complement(1781..2872)
                     /locus_tag="VNU03_00665"
     CDS             complement(1781..2872)
                     /locus_tag="VNU03_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWP72984.1"
                     /translation="MTGTTAAPLGAGSRRVLIVDDELDIRATLRAAFGSAGYECRIAG
                     NGQEAAVLYDTERAPLTITDLKMPVMDGLELLRHVRARDADAAVIMLTGAGDVAVAVE
                     SLKQGASDFILKPVDLDQILLSAERALERRQLLIEHREHHVLLEYRVAEATRELAAAL
                     GELQQTYLATLEALGSALATREVGTELHSRRVHAYSIALARAHRLPEEELEALGRGVL
                     LHDIGKIGISDSILLKPGPLTPEEWQVMRRHPEVGRQLLERIPFLRNAIPVVYHHHER
                     WDGTGYPMGLAGPAIPLGARIFAVADAFDALTYDRPYSKAIPMEAARERIRIAAGTHF
                     DPDVVATFLAMPLAVFEMARARPLDLPIA"
     gene            complement(2875..3903)
                     /locus_tag="VNU03_00670"
     CDS             complement(2875..3903)
                     /locus_tag="VNU03_00670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWP72985.1"
                     /translation="MPKRPTAPDTPAARRIKRKILIAITLTAFLPMLVVAYLVYSYAF
                     PLLDVQTQERDLPWMLVMLCGTGLLMASGSFMLWDLATSVVRHADTNTSSRPVTKPGT
                     PAPVASPETAAVQQYVQQSDEVDSLMQSFSRMLVTIEQQAADVNEYARRLDTAYKDLE
                     STSAQLKEFSFKDEVTGLYNRRFFSIRLEEEVSRYRRFNHPVSVVLLDLDGFKAVNDD
                     LGHGAGDETLRAMADILLKQSRGINVICRYGGDEFAVLLVETSKSGARLYADRIRYVL
                     STWAFAHGRRVTASFGIASLPEDVAPAADELIRAADEALYAAKRAGKNRVSVHEDIGA
                     APGVRPGA"
     gene            4032..4283
                     /locus_tag="VNU03_00675"
     CDS             4032..4283
                     /locus_tag="VNU03_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWP72986.1"
                     /translation="MVLERKLVNPDRQIDCTGLFCPMPIVKTREAITQMGVGELLEML
                     SDDPASEPDMRSWARNTGHDLLEITRSGIVYRFVVRKTR"
     gene            4289..>4893
                     /locus_tag="VNU03_00680"
     CDS             4289..>4893
                     /locus_tag="VNU03_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWP72987.1"
                     /translation="MAPEAVEPERVYLDYAGFAPVDPRVLAVMRPFLEAGIGNPSARH
                     SLGAEARESLDAARVKVARLCGGAAGGIVFTSGATEANNLAIKGVALRARAGATRVVL
                     SAVEHISVINACRDLEKAGAVLCALPVDGDGRVDPGMLRAVLTPDTTLVSIAAANPEI
                     GTVQPLKEIGRVTREAGVPLHVDGVGALGRVPLNVETMGIDL"
BASE COUNT          731 a         1579 c         1759 g          824 t
ORIGIN      
        1 agagaacgag caaggcagcc aggatcccgc cggtcgtact cgtcaagcgc cggccaagca
       61 gaaacgtgag gcaaaccatc attccaaagg ccgcgacgga gatgagccag gtggccctga
      121 tcggcgagac gccgagccaa acaatcggga ccgcgatcaa cgaatagccg ggcggccaca
      181 gcacatactg ggtgagggcg tctcgcatgc gcccggaagc catgagcaag gtttcatggg
      241 cgtagctata gctcaacgga gtcaggccga taccacctcg gcccgccgcc gtcgccgccg
      301 cgagaccggt gaggaaaagg atgatcggcc cggcgaatcc cgccgaactt agagcgatcg
      361 gctgaccgcg gacccaccgc aggagaccat tctcgagcag gttcacgggc gcaatgctag
      421 cccgcgtggt ccgccgcggt caacttcgcg ggtaggcgcc cggcgggcgg accgctgtgg
      481 ccggtgtgtg acgtaacaca atcttgagga gttgcgtccc cgggcgccct tgacgccact
      541 ctcgcgaaca ggtagccttc tgggcctcgt cacgaagtgt gccgcaaccg ctaccgtgga
      601 gaactcatgc aatcaagtcc gggatcgccc ctcctctctc atattgccgc ccttgacggt
      661 cttcgggggc tggccattct gagcgtgatg ttcttccatg caaccctttc acgtgcgaac
      721 gctcctcggc cgagcggagg attcttgggc gtagacgtgt ttttcgtact cagtggtttt
      781 ctgatcaccg ctgtgctgtt gaaggaatgg gatcagaagg gctcgatagc cttgcggtcg
      841 ttctacgcgc ggagagcact ccggctgctg ccggcgcttt tcgtcttgat ggcggtgatc
      901 ctgctggtgc cgggggtgtt ctactcgtcc cagcggccgt ggagggacgc gatggtcgcg
      961 gcggcctacg caacgaactg ggttcgcgcg ttcggcggag acatcggatt cttcggccac
     1021 acgtggtccc tgaccatcga ggagcagttc tatgtcatct ggccgctgct gttggtgggt
     1081 cttcttcgac tgagagtccg ccggcgcgtg attctgctcc tggtcctggt ggctatcgcg
     1141 gtcccgaccg tgctgcgcgt ggatctgtgg gagggaccca tgtcggtcaa gcgcctttac
     1201 tacgggctcg atacccggtt cgattcgctc ctgttgggct gtctggtcgc gttcctcgct
     1261 tcgtggaacg tcatccctcg aagtgagcgc gcactgaaga tgatccggtt ggccgccgcc
     1321 gcggcggcgg tggtcctggt ggtgctgatt gccaacgcca ccgaggaatc gcagctgacg
     1381 tatttcggtc tcggggtgat cgcatgcgcc tcggtggccg tccttctcgt acacctaatc
     1441 tactgcccat cgcgcctgtc cgatctcatc ctcgcgaacc ggccgatggt ctgggtcggc
     1501 cgaatctcct atggccttta tctctggcac gacccgatct tcttcgggat gctcaaccct
     1561 actcgcatgg ggaaagtcgg agtgactggg gtcggactta tggccgtccg attcgtggct
     1621 gccttcgtgg cagccaccgt ctcgttctat ctgctcgagc gaccgcttct gcggatcaag
     1681 gatcatctgg ccaggcccgg tgcggagccg gccggctcgg ccctcgtcga ggggccgcga
     1741 taggatccgc ctctcggcga ccgacgggag acgaggccgg ctaggcgatc gggaggtcga
     1801 gcgggcgggc gcgggccatc tcgaagaccg cgagaggcat cgcgaggaac gtggccacca
     1861 cgtcgggatc gaagtgggtc cccgccgcga tccggatccg ctcccgggcg gcctccatcg
     1921 ggatggcctt cgagtagggg cggtcgtagg tgagcgcgtc gaacgcgtcc gcgaccgcga
     1981 agatccgagc acccagcggg atcgcgggcc cggccaggcc catcgggtag ccggtgccgt
     2041 cccaccgctc gtggtggtgg tacacgaccg ggatcgcgtt gcgcaggaac gggatccgct
     2101 cgagcagctg ccggccgacc tccgggtgcc gccgcatcac ctgccactcc tcgggagtca
     2161 gcggacccgg cttgaggagg atcgagtccg agatgccgat cttgccgatg tcgtgcagga
     2221 ggacgccgcg gccgagcgcc tccagctctt cctccggcag gcggtgcgct cgggcgagcg
     2281 cgatcgagta ggcgtgcacg cgccgggagt ggagctcggt gcccacctcc cgggtggcga
     2341 gcgcggagcc cagggcctcg agggtcgcca ggtaggtctg ctgcagctcg ccgagggcgg
     2401 cggccagctc gcgggtggcc tccgccaccc ggtactcgag gaggacgtga tgctcgcggt
     2461 gctcgatcag gagctgccgg cgctcgagcg cgcgctcggc ggagagcagg atctggtcga
     2521 ggtcgaccgg cttcaggatg aagtcgctcg ccccctgctt gaggctctcc accgccaccg
     2581 cgacgtcgcc ggcgccggtg agcatgatga ccgccgcgtc ggcgtcgcgg gcccggacgt
     2641 gccggaggag ctcgaggccg tccatcaccg gcatcttgag atcggtgatc gtgagcggcg
     2701 cccgctcggt gtcgtacagc accgccgctt cctgcccgtt gcccgcgatg cggcactcgt
     2761 agccggcgga gccgaaggcg gcccggagcg tggcgcggat gtcgagctcg tcgtcgacga
     2821 tgaggacgcg ccggctaccg gctccgagag gcgccgcggt ggtgccagtc accgtcacgc
     2881 tccgggccgg accccggggg cggcgccgat gtcctcgtgg acggagacgc gattcttgcc
     2941 ggcccgcttc gccgcgtaga gcgcctcgtc ggcggcgcgg atcagctcgt cggcggcggg
     3001 ggccacgtcc tcgggcaggg acgcgatgcc gaagctggcg gtcacccggc ggccgtgggc
     3061 gaaggcccag gtggagagca cgtagcggat gcggtcggcg tagagccggg ccccgctctt
     3121 cgaggtctcg accagcagca ccgcgaactc gtcccccccg tagcggcaga tgacgttgat
     3181 gccgcgggac tgcttgagga ggatgtcggc catcgcccgc agcgtctcgt cgccggcccc
     3241 gtgtccgaga tcgtcgttga ccgccttgaa gccgtccaga tcgagcagga ccaccgagac
     3301 cggatggttg aagcggcgat agcgggagac ctcttcctcg agccggatgg agaagaagcg
     3361 gcggttgtag aggccggtga cctcgtcctt gaacgagaac tccttgagct gggcgctcgt
     3421 ggactcgaga tccttgtagg cggtgtcgag gcggcgcgcg tactcgttga cgtcggcggc
     3481 ctgctgctcg atggtgacca gcatgcgcga gaaggactgc atgagggagt ccacctcgtc
     3541 gctctgctgc acgtactgct ggaccgccgc ggtctccggg ctcgccaccg gggccggcgt
     3601 gccgggcttg gtcaccgggc ggctgctggt attcgtgtcg gcatgccgca ccaccgaggt
     3661 ggccaggtcc cagagcatga agctgccgga ggccatcagg agcccggtgc cgcagagcat
     3721 gacgagcatc cacgggagat cgcgctcctg ggtctgcacg tcgaggagcg ggaacgcgta
     3781 ggagtagacc aggtacgcga ccaccagcat cggcaggaac gcggtgaggg tgatggcgat
     3841 caggatcttg cgcttgatgc ggcgtgcggc gggggtatcc ggagccgtcg ggcgcttggg
     3901 catatctagc cgcctccagg atatcggcgt cggggctggg cggtgcggga atttcacgcg
     3961 ccctcggccg gaacgtcacc ggcggcccct tccttgacgg ggtgacgatg ggtggggtac
     4021 ggtaggctcc gatggtcctg gagcgcaaac tcgtcaatcc tgacaggcag attgactgca
     4081 ccggcctgtt ctgtccgatg cccatcgtca agacccgcga ggccatcacg cagatggggg
     4141 taggggagct cctcgagatg ctgtcggacg atccggcctc ggaacccgac atgcggagct
     4201 gggcccggaa caccggtcac gatctgctcg agatcacgcg gagcgggatc gtctaccgct
     4261 tcgtggtccg gaagacgcgg tagccacggt ggcgcccgag gcggtcgagc ccgagcgcgt
     4321 ctacctcgac tacgcgggct tcgcgcccgt cgatccgcgg gtcctcgcgg tgatgcggcc
     4381 gtttctcgag gcgggcatcg gcaatccctc cgcgcgccac tcgctcggcg cggaggcgcg
     4441 cgagtccctc gacgccgcgc gggtgaaggt ggcgcgcctc tgcggcggcg ccgcgggcgg
     4501 gatcgtgttc acctccgggg ccaccgaggc gaacaacctg gccatcaaag gcgtggcgct
     4561 gcgcgcgcgc gccggcgcca cgcgcgtggt gctctccgcg gtggagcaca tctcggtgat
     4621 caacgcgtgc cgcgacctcg agaaggccgg cgccgtcctc tgcgccctgc cggtggacgg
     4681 ggacgggcgc gtcgatcccg gcatgctgcg cgcggtcctg accccggaca cgaccctcgt
     4741 ctccatcgcg gccgcgaacc cggagatcgg gacggtgcag ccgctcaagg agatcggccg
     4801 ggtcacccgg gaggcgggcg tgccgctcca cgtggacggg gtcggagcgc tggggcgcgt
     4861 gccgctcaat gtcgagacga tggggatcga tct
//
LOCUS       DATMCV010000025         6012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_466717, whole genome shotgun sequence.
ACCESSION   DATMCV010000025 DATMCV010000000
VERSION     DATMCV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466717"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VNU03_00685"
     CDS             complement(<1..879)
                     /locus_tag="VNU03_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP72988.1"
                     /translation="MSERETRPHVVIVGGGFGGLYAARALAERPVRVTLLDRRNHHLF
                     QPLLYQVATAALNPSDIATPLRSILRGASNVTVFLAEVESVDLAGRRLVLDEGPMTYD
                     ALVLAAGAGHSYFGHDDWEPLAPSLKTLEDALEIRRRVLVAYEEAEREQDGAEQRALL
                     TSVVIGGGPTGVELAGALAEISRETIARDFRVIDPTKARIVLLEGGPRVLAAFPDPLP
                     ARAAAALARIGVEVRTGATVTRVTSDAVWLGGEQIRARTVLWAAGVAAAPLTRTLGVP
                     LDRAGRVLVERDLSIPG"
     gene            1016..1930
                     /locus_tag="VNU03_00690"
     CDS             1016..1930
                     /locus_tag="VNU03_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HWP72989.1"
                     /translation="MIKLSDVKVRPQYRTRVRYRLLVLEYAWQHGPAAAGRHYGLSAR
                     TIRRWRKRWRRGGIEGLVPSYPRHRARRVSPAAVELMRQARQEFGYGAARTRLWLQRV
                     HGIRLAMGTIQRVFRDLGLPRLRRTRKREPRQMKLFEKAEPGESIQVDVKYVQIAGRW
                     AFQYTALDDCTRFRVLRLYRRLHHRSSLAFLTELRTAFPFPIQRLQCDHGQEFSFAFV
                     LGVEAAGIRHRYIRPRRPQQNGKVERSHRIDQEEFWGRQRFPDFEAAAAALRGWETRY
                     NYERFSLALPGRTPAEKLAALQPPRRAA"
     gene            2044..2661
                     /locus_tag="VNU03_00695"
     CDS             2044..2661
                     /locus_tag="VNU03_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HWP72990.1"
                     /translation="MFRLLPREEKFFDLFEQQATHIVSAARTLEELTLDYADAKAKAQ
                     QIKDLEHAGDTLTHEIVRRLNTTFITPIDREDIYALASRLDDVLDLIDAVADRLLLYK
                     IKAPTDGSIGMAKIIVKTAEETDRAVRCLRTLSPFYHKHAVEVNRLENEADRLLRDQL
                     AALFEGGTDAIEVIKWKELYETMESVTDRCEDVVNVIEGITLKMA"
     gene            2681..3688
                     /locus_tag="VNU03_00700"
     CDS             2681..3688
                     /locus_tag="VNU03_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HWP72991.1"
                     /translation="MSGSLLLVVFIILVALAFDYINGFHDAANSIATVVSTRVLTPMQ
                     AVAWAAFFNFVAAFGFGVQVARTVGKGVVDAGLVDQWVILGGLVGAITWNLITWYYGI
                     PSSSSHALIGGYAGAAVAKAGFGALVPSGLLKILVFIVLAPVLGLCLGFPLMVITLWT
                     VRSIRPSRVDGLFRRLQLLSAAAYSLGHGTNDAQKTMGIIAILLFSAGYLGSEFYVPM
                     WVILAAHAAIGLGTLAGGWRIVKTMGMRLTKLRPIGGFCAETAGAIMLIGTAVGGIPV
                     STTHTITGSIMGVGATQRLSAVRWGVASNIVWAWILTIPLSGLIAAAVWGVTNLILRA
                     G"
     gene            3685..4593
                     /locus_tag="VNU03_00705"
     CDS             3685..4593
                     /locus_tag="VNU03_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWP72992.1"
                     /translation="MSASPRAGRLAATVGLWGLRLVGWLPAHLPPAAGLAAGRWIGDL
                     LWWLLPGRRRRALDNIRLSLGREMTPREIERLGRRSFQHLGMNLIEACRYFLRPTEVM
                     LSQVHVEGVEHLQSAAARGRGVLILTAHYGNWELLAAAHGLSGLPLSIVIRPLDHPVL
                     DELAARFRRRSGAELIVKRQAVREVLQALRRGRMVGILLDQNATRAEGVFVPFFGVAA
                     STSKGLAVLALRTDAPVVPAFLRRAADGRHCMDVSAPLEPPPDGDVVAYTAMFNRVIE
                     GAIRRAPEQWLWMHARWRTRPPGAGS"
     gene            4560..5291
                     /locus_tag="VNU03_00710"
     CDS             4560..5291
                     /locus_tag="VNU03_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72993.1"
                     /translation="MADPSAGGGLVTGACLGRAWIIAGGLLLLAGPGATADESVAGLV
                     APYLKARGAGTLGTVAAKAYAEPTRPSAAPTPPPSVSVTLVPYSAALEAELDAVKGGL
                     RDSLDGYVQAVARIEDARVDYQRALLEAGAGELVRTQLTDGQGEARVGELPVGDWLVL
                     AWREGGHLSKRFKLRDTEASRYPNVPSNVTYSTVTYWRTRVTVRAGEAAEVTMSDRNV
                     WLTAARQEGGSPVTPRRPQGSKQRR"
     gene            complement(5334..5585)
                     /locus_tag="VNU03_00715"
     CDS             complement(5334..5585)
                     /locus_tag="VNU03_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP72994.1"
                     /translation="MKKLVALVAGIAFSLGIGLAIPQTLAQTPAPKEEKKGEMDKMDK
                     DKMEKKGEKKGAAKKKKSDKADKDKMDKMDKMEKKEDKK"
BASE COUNT          881 a         2095 c         2054 g          982 t
ORIGIN      
        1 gccggggatc gagaggtcgc gctcgacgag cacgcgcccg gcgcggtcga gcggcacgcc
       61 gagcgtgcgg gtgagcggtg cggcggccac tcccgcggcc cacagcacgg tgcgcgcgcg
      121 gatctgctcg ccgcccagcc agaccgcgtc ggaggtcacg cgcgtcacgg tggcgccggt
      181 gcggacctcg accccgatgc gggcgagcgc ggcggccgcg cgcgcgggca ggggatcggg
      241 aaaggcggcc aggacgcggg ggccgccctc gagcagcacg atgcgcgcct tggtggggtc
      301 gatcacgcgg aagtcgcgcg cgatcgtctc gcgcgagatc tcggcgagcg cgccggccag
      361 ctccaccccg gtcgggccgc cgccgatcac caccgacgtg agcagcgcac gctgctccgc
      421 gccgtcctgc tcgcgctcgg cctcctcgta ggccaccagc acgcggcgcc ggatctcgag
      481 ggcgtcctcc agggtcttca ggctcggggc gagcggctcc caatcgtcgt ggccgaagta
      541 ggagtggccc gcccccgcgg ccagcacgag cgcgtcgtag gtcatcgggc cctcgtcgag
      601 gacgaggcgg cggccggcca ggtccaccga ctcgacctcg gccaggaaga cagtgacgtt
      661 ggaggccccg cgcaggatcg agcgcagcgg tgtcgcgatg tcggacgggt tgagggccgc
      721 ggtggccacc tggtagagca ggggctggaa gaggtggtga ttgcggcggt cgagcagggt
      781 gacgcggacc ggccgctccg cgagcgcgcg ggccgcgtag agcccgccga acccgccgcc
      841 cacgatgacc acgtgcggcc gcgtctcgcg ctcgctcacc gcctcatctt ccgggcgacc
      901 cgggacggtg tcaaccgagc gcgcgccgcg gccggctgaa gtatctactc aagattgacc
      961 tcctttgctt tgataggcag ctgggcgtcc agcctgtcca aaccaagggg gggccatgat
     1021 caagctcagc gacgtgaagg tgcggccgca gtatcggacg cgggtgcggt atcgcctgct
     1081 ggtgctggag tatgcgtggc agcacgggcc cgccgccgcg ggccggcact acggactcag
     1141 cgctcggacg attcggcggt ggcggaagcg ctggcggcgt ggaggcatcg aggggctggt
     1201 gccctcctat ccgcggcacc gggcgcggcg agtatcaccc gcggccgtcg agctcatgcg
     1261 ccaagcccgc caggagttcg gctacggggc ggcgcgcacc cggctctggc tccagcgcgt
     1321 gcacgggatc cgattggcca tgggcacgat tcagcgggtg tttcgcgacc tgggcctccc
     1381 ccgcctgcgg cggactcgca agcgggaacc ccgacagatg aaactttttg agaaggcgga
     1441 accgggggag tcgattcagg tcgacgtgaa gtatgtgcag atcgcgggcc gctgggcatt
     1501 tcagtacacg gccctggacg actgcacgcg ctttcgcgtc ctgcggctct atcgacgctt
     1561 acatcaccgc tcgagcctcg ccttcctgac cgagctccga acggccttcc cgttccccat
     1621 ccagcgcctg cagtgtgatc acggccaaga gttctccttc gcctttgtgc tgggggtcga
     1681 agccgccggg atccgccatc ggtatatccg accccgccgg ccccaacaga acggcaaggt
     1741 cgaacggagc catcggatcg atcaggagga gttctggggc cggcaacgct tcccggactt
     1801 cgaggcggcc gccgccgccc tgcggggctg ggagaccagg tacaactatg aacgcttctc
     1861 cctcgctctg ccgggccgca ccccggccga aaaacttgcc gcgctccagc cacctcggcg
     1921 ggccgcttag gcgtctcgtg cctgcgcctg ctcgacaccg aaacccgggg gtcaatcttg
     1981 acgagaccca acaaccgacc gcccggcgtt gacatcggcc ggacgccggg agaggatact
     2041 gccgtgttcc ggctgcttcc gcgggaggag aagttcttcg atctgttcga gcagcaggcc
     2101 acgcacatcg tgtccgcggc gcgcacgctc gaggagctga ccctcgacta cgcggacgcc
     2161 aaggcaaaag cccagcagat caaggacctg gagcacgcgg gcgacaccct gacccacgag
     2221 atcgtgcgcc ggctcaacac cacgttcatc acgcccatcg accgtgagga catctacgcg
     2281 ctcgccagcc gcctggacga cgtgctggac ctgatcgacg cggtggcgga ccggctgctc
     2341 ctgtacaaga tcaaggcccc gaccgacggc tccatcggca tggccaagat catcgtgaag
     2401 accgcggagg agaccgaccg ggcggtgcgc tgcctccgga cactgtcgcc cttctatcac
     2461 aagcacgcgg tggaggtgaa tcgcctggag aacgaggcgg accgtctgct gcgcgatcag
     2521 ctcgccgccc ttttcgaggg gggcaccgac gccatcgagg tcatcaagtg gaaagagctg
     2581 tacgaaacca tggagtcggt gaccgatcga tgcgaggacg tggtcaacgt gatcgaggga
     2641 atcaccctca agatggcctg acccggcccg cccgccgcgc atgagcgggt ccctgctgct
     2701 ggtggtgttc atcatcctcg tcgcgctcgc cttcgactac atcaacggct ttcacgacgc
     2761 ggccaactcg atcgccaccg tcgtctccac ccgggtcctc accccgatgc aggcggtggc
     2821 gtgggccgcc ttcttcaatt tcgtggccgc cttcggcttc ggggtacagg tggcgcgcac
     2881 ggtgggcaag ggagtggtcg acgcgggcct ggtggaccag tgggtgatcc tgggcgggct
     2941 cgtcggcgcc atcacctgga acctcatcac ctggtactac gggatcccgt cgagctcctc
     3001 ccacgcgctc atcggcggat acgccggcgc ggcggtggcg aaggcgggct tcggggccct
     3061 cgtgccgagc ggtctcctga agatcctggt cttcatcgtg ctcgcgcccg tgctcggcct
     3121 ctgcctgggc ttccccctga tggtgatcac cctctggacg gtacggtcga ttcgacccag
     3181 ccgggtggac ggcctcttcc gccgactgca gctgctctcc gccgccgcct acagcctggg
     3241 tcacggaacc aacgacgcgc agaagacgat ggggatcatc gcgatcctcc tcttttccgc
     3301 cggctatctc gggtccgagt tctacgttcc catgtgggtg atcctggccg cgcacgccgc
     3361 catcgggctg ggcaccctcg ccgggggctg gcgcatcgtc aagaccatgg ggatgaggct
     3421 gaccaagctg cgcccgatcg gcggcttctg cgccgagacc gccggagcga tcatgctcat
     3481 cggcaccgcg gtgggcggca tcccggtcag caccacccac accatcaccg gctcgatcat
     3541 gggggtcggc gccacccagc ggctctccgc ggtccgctgg ggagtcgcga gcaatatcgt
     3601 ctgggcctgg atcctgacca tcccgctctc cgggctgatc gcggcggcgg tctggggtgt
     3661 caccaatctg atccttcgcg ccggatgagc gcgtccccac gcgccggccg gctggcggcg
     3721 accgtgggtc tctggggcct ccggctggtc ggctggttgc cggcccatct gcctccggcg
     3781 gcgggcctgg ccgccggccg gtggatcggt gatctcctct ggtggctcct gcccggccgt
     3841 cgccgccggg ccctcgacaa catccgcctg agcctcggcc gggagatgac gcctcgagag
     3901 atcgagcgac tcgggcgacg atcgttccag cacctgggca tgaacctgat cgaagcttgt
     3961 cgctacttcc tccgtcccac agaggtcatg ctctcgcagg ttcacgtcga gggggtcgag
     4021 catctgcaga gcgccgccgc ccggggccgg ggcgtcctga tcttgaccgc ccactacggc
     4081 aactgggagc tgctggcggc cgcccacggg ctgagcggcc tgcccctgtc catcgtgatc
     4141 cgacccctcg accatccggt tctcgacgag ctggccgccc gcttccggcg acgcagcggc
     4201 gccgagctca tcgtgaagcg ccaggcggta cgggaagtcc tgcaggccct gcgacgggga
     4261 cgcatggtcg gcatcctgct cgaccagaac gccacgcgcg ccgagggcgt gttcgtgccg
     4321 ttcttcgggg tcgcggcctc gacgtcgaag ggactggccg tcctcgctct ccggaccgac
     4381 gctccggtgg tgccggcgtt cctgcggcgg gcggcggatg ggcggcattg catggacgtg
     4441 agcgctccgc tcgagccgcc gcccgacggt gacgtcgtcg cctacaccgc gatgttcaac
     4501 cgggtcatcg agggggcgat ccgtcgtgct ccggagcagt ggctctggat gcacgcgcgg
     4561 tggcggaccc gtccgccggg ggcgggctcg tgaccggggc ttgcctcggc cgggcctgga
     4621 tcatcgcggg cggcctgctc ctgctcgccg gccctggagc gacggccgac gagtcggtcg
     4681 cgggactcgt cgcgccctac ctgaaagcgc gcggtgccgg gaccctgggc accgtggcgg
     4741 cgaaagccta cgccgagcct acccgcccga gcgcggcccc gacgccgccg ccctcggtct
     4801 cggtcaccct ggtcccgtac tcggcggccc tcgaggccga gctggacgcg gtcaagggcg
     4861 ggctccgcga ctccctcgac ggttacgtgc aggcggtggc ccggatcgag gatgcgcggg
     4921 tcgactacca gcgggcactg ctcgaggcgg gcgcggggga gctggttcgc acccagctga
     4981 ccgacggcca gggggaggcg cgggtcggcg agctgccggt gggcgactgg ctggtgctgg
     5041 cctggcggga gggaggtcac ctctcgaagc gcttcaagct acgcgatacg gaggcatccc
     5101 gttacccgaa cgtcccgagc aacgtgacct actcgacggt gacctactgg cgcacgcggg
     5161 tcacggtgcg ggcgggggaa gcggccgagg tcacgatgag cgaccggaac gtgtggctca
     5221 ccgctgctcg ccaagaaggc ggcagcccgg tcacgccgcg gcgcccccag gggtccaagc
     5281 agcgccgcta gggcgccacc cgaccgaggt ggcgcccctg ggtcgcggct gcgctacttc
     5341 ttgtcttcct tcttctccat cttgtccatc ttgtccatct tgtccttgtc cgccttgtcc
     5401 gacttcttct tcttggcggc acccttcttt tcgcccttct tctccatctt gtctttgtcc
     5461 atcttgtcca tctcgccctt cttctcttcc ttgggggcgg gagtctgggc gagcgtctgc
     5521 gggatcgcga gaccgatacc gagcgagaat gcgataccgg caaccagggc cacgagcttc
     5581 ttcatagcgc gtcacctcct ttcgacattg actggactaa cacaaaaagg cctttcataa
     5641 aggcgactta ggatatgagc cgcccccccg ggtgtcaaga aatattctgc gagattcggc
     5701 agggcctgac cccgttgccc taaatatacg gtcgtgataa aaagttcatg ccacctcagt
     5761 atctgatggg tccttcaatc cggcggctgc tgatcgttgt cgtgctcctc acacccggtt
     5821 acctggctct gccagctaca gccgggacgg agcccgagac ggcatctgct tggacgggag
     5881 agcccggagc cgagccagcc gcggtcgctc ccatcgaaga tgccggattg gcctccgttc
     5941 ctcagccctc gatctccacc gatgccccgc tcaccctgga tgccgatctc tgggccgagt
     6001 ccttcacgac tc
//
LOCUS       DATMCV010000026         3910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_466912, whole genome shotgun sequence.
ACCESSION   DATMCV010000026 DATMCV010000000
VERSION     DATMCV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466912"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1375)
                     /locus_tag="VNU03_00720"
     CDS             complement(20..1375)
                     /locus_tag="VNU03_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP72995.1"
                     /translation="MKPRILVVDDETVIAENLRLTLEREGHDVETAANTVGAMLRMET
                     REFALAIVDLILPDGDGLHLLRLLKAKDPSIEVIIMTGHGSISKAVEATKQGAFYFVA
                     KPFDADEMLMLVSKALERRRLLAETSDLRRKLAEQAAYGEMLGSSPAIKRVFEMLESV
                     ASSDANVLIVGESGTGKELAANAVHAKSTRVDGPMVKINCAALPKDLIESELFGHVKG
                     AFTGASTDKPGLLEEAHRGSLLLDEITEMPLDLQAKLLRVLEDRTVRRLGGAKTVPVD
                     FRLICSTNREPEAALRDGRLRQDLYFRINTVTVTLPSLRERSGDIPLLAKSFLERFRL
                     KHSREVDTIDPEAYRRLLSYSWPGNIRELEHAVERAVLVARGKEIALSDLPEPLQANA
                     SPEGSAPPPGSLEEIERVSIVRALESTGWNKQAAAQVLGLRRPTLYSKMRRHGIPQRR
                     A"
     gene            1515..2480
                     /locus_tag="VNU03_00725"
     CDS             1515..2480
                     /locus_tag="VNU03_00725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP72996.1"
                     /translation="MHFGVSLPGRGPLATPEILTKLAEKADALRYTSLFVTDHVVIPT
                     ANNSTYPYSPSGRFATDWTNGYLEPIAMMGVLVGLTSRIKLGTSVLVVPYRNPVVTAK
                     MLATLDVMSGGRLILGAGVGWMREEFEAVQAAPFEQRGQVTDEYLRLMRVMWTKEPAE
                     FAGKYYRLPSVSALPKPRQAGGIPIWVGGHTDAALRRTGELADAWHPIGMRPPAMLHP
                     PEYAQKVQAIHGFARKAGRDPKDITLSLRVPLELAPRRGAWTGGDQPGFRGTAAQVIQ
                     HIKAYQALGVSHFVFDLAAPDLRGQLSLMERFVEEVRPKVLRAPR"
     gene            2489..2938
                     /locus_tag="VNU03_00730"
     CDS             2489..2938
                     /locus_tag="VNU03_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HWP72997.1"
                     /translation="MPSTLAAPLPGPVAGFISSARIGRLATADAGGQPMVVPICYAFD
                     GTALYSAVDAKPKTAPPEGLKRIRNLRENPRVSIVIDEYQEDWTKLRYVIIQGQADLL
                     TDGPQFAHGVDLLVAKYSQYRALGLARERGLMIRVTPSRVSAWQYSS"
     gene            2920..>3910
                     /gene="cofG"
                     /locus_tag="VNU03_00735"
     CDS             2920..>3910
                     /gene="cofG"
                     /locus_tag="VNU03_00735"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HWP72998.1"
                     /translation="MAVLELSPVDRALDRVAEGRVPSAAEATALLDTPAPRLQTLLDA
                     AAALRDRGHGRRITFSAKVFIPLTTLCRDYCGYCTFRKDPGEPGAFTMTPEQVLALAH
                     AGARLGAKEALFSLGDKPEALFPEHRAFLRRMGHRTTLGYLREVSARVLEETGMLPHA
                     NPGLMSERDLAALREVNVSMGIMLESTSERLLAPGAAHDRAPDKVPARRLRTIARAGA
                     LGIPFTTGILIGIGETHAERVESLLAIRDLHERHGHIQEVIVQNFRAKPTIPMRGWPD
                     PGSVDLLRTVAVARLLLGPAMNIQAPPNLSADGYERLAAAGLNDWGGVSPLTPD"
BASE COUNT          567 a         1437 c         1319 g          587 t
ORIGIN      
        1 gggcgtctcg aagcgaacgt caggcgcggc gctgcgggat cccgtggcgg cgcatcttcg
       61 agtagagcgt ggggcggcgc aggcccagca cctgggccgc ggcctgcttg ttccagccgg
      121 tggactcgag cgcccgcacg atcgagaccc gctcgatctc ctcgagcgat cccgggggcg
      181 gcgccgaccc ctcgggcgag gcgttggcct ggagcggctc cggcaggtcg ctcagcgcga
      241 tctccttgcc ccgggccacc agcaccgccc gctcaaccgc atgctccagc tcgcggatat
      301 tgcccggcca cgagtacgac agcagccgcc ggtacgcctc gggatcgatg gtgtcgacct
      361 cgcggctgtg cttcagccgg aagcgctcca ggaaggactt ggcgaggagg gggatatccc
      421 ccgagcgctc ccgcagggac ggcagggtca cggtgaccgt gttgatccgg aagtagaggt
      481 cctggcggag acgcccgtcg cgcagcgccg cctccggctc ccggttcgtc gagcagatca
      541 gccggaagtc gaccggcacc gtcttggcgc cacccagccg gcggaccgtg cggtcctcga
      601 ggacccgcag cagcttggcc tggaggtcga gcggcatctc ggtgatctcg tcgagcagca
      661 gcgagccgcg gtgcgcctcc tccaggaggc ccggcttgtc ggtggacgcc ccggtgaagg
      721 cccccttgac gtggccgaac agctcggact cgatcaggtc cttcgggagc gccgcgcagt
      781 tgatcttcac catcggcccg tccacccggg tgctcttggc gtggaccgcg ttggcggcca
      841 gctccttgcc ggtcccgctc tcgcccacga tgagcacgtt ggcgtcggac gatgccaccg
      901 actccagcat ctcgaagacc cgcttgatgg ccggggagga cccgagcatc tccccgtagg
      961 ccgcctgctc cgccagcttg cgccgcagat cggaggtctc ggcgaggagc cggcggcgct
     1021 cgagcgcctt gctcacgagc atcagcatct cgtcggcgtc gaagggcttg gccacgaagt
     1081 agaaggcgcc ctgcttggtc gcctccaccg ccttcgagat cgacccgtgc ccggtcatga
     1141 tgatgacctc gatcgacggg tccttcgcct tgagcaggcg gaggagatgc agaccgtccc
     1201 cgtcgggcag gatcaggtcg acgatggcga gggcaaactc gcgcgtctcc atgcggagca
     1261 tggcgccgac ggtgttggcc gcggtctcga cgtcgtgccc ctcacgctcg agagtcaggc
     1321 ggagattctc cgcgatgacg gtctcgtcgt cgacgaccag gatccgaggc ttcatggatt
     1381 cgagcctagc acaggcggat tttttccgtg tcacccacgt gacacttcgc cctcggtgcc
     1441 gcgagccctc gcccggcgcc gcgcgaaaag cccgcgcctt gacaggccga aagcgcgcga
     1501 gcaatatgcg gggcatgcat ttcggcgtct cgctgccggg ccgtggtccg ctcgcaacac
     1561 ccgagatcct caccaagctg gccgagaagg cggacgcgct tcgctacacc tcgctcttcg
     1621 tgaccgacca cgtggtcatc ccgaccgcca acaactcgac ctacccttat tctcccagcg
     1681 gccggttcgc gacggactgg accaacggct acctggagcc catcgccatg atgggcgtgc
     1741 tggtcggcct gacctcgcgc atcaagctgg gcaccagcgt gctggtggtc ccctaccgca
     1801 acccggtggt gacggccaag atgctcgcga cgctcgacgt catgtcgggg ggccgcctga
     1861 tcctgggcgc cggggtgggc tggatgcgtg aggagttcga ggccgtgcag gcggcgccgt
     1921 tcgagcagcg cggccaggtc accgacgagt atctccgtct catgcgcgtg atgtggacga
     1981 aggagcccgc cgagttcgcg ggcaagtact accggctgcc gtcggtgagc gcgctgccca
     2041 agccccggca ggccgggggc atccccatct gggtgggtgg gcataccgac gccgcgctgc
     2101 ggcgcaccgg cgagctcgct gacgcctggc accccatcgg gatgcggccc cccgcgatgc
     2161 tgcacccgcc cgagtacgcc cagaaggtcc aggccatcca cggcttcgcg cgcaaggccg
     2221 gacgcgatcc caaggacatc acgctctcgc tgcgggtgcc gctcgagctc gcgccgcggc
     2281 gcggagcctg gaccggcggc gaccagcccg gcttccgcgg caccgccgcg caggtgatcc
     2341 agcacatcaa ggcctatcag gccctcggcg tctcgcactt cgtgttcgac ctggccgcgc
     2401 ccgatctccg cggccagctg agcctcatgg agcgcttcgt cgaagaggtc cgtccgaagg
     2461 ttctgcgggc tccccgctga gcgccccgat gccgtcgacg ctggccgctc ccctgccggg
     2521 ccctgtcgcc ggcttcatct cgagcgcccg gatcgggcgc ctcgcgaccg ccgacgcggg
     2581 cggccagccg atggtggtgc cgatctgcta cgccttcgac ggcaccgcgc tctactccgc
     2641 ggtggatgcc aagcccaaga cggctccccc cgaggggctc aagcgcatcc gcaaccttcg
     2701 cgagaatccg cgcgtctcaa tcgtgatcga cgagtaccag gaggactgga cgaagctccg
     2761 atacgtgatc atccagggtc aggccgatct cctcaccgac ggtcctcagt tcgcccacgg
     2821 ggtcgacctg ctggtcgcca agtactccca gtaccgcgcc ctcggcctcg cccgcgagcg
     2881 cgggctcatg atccgcgtga ccccgagccg ggtgtcggca tggcagtact cgagctgagc
     2941 ccggtcgatc gggcgctgga ccgcgtggcc gagggccggg tgccctccgc cgcggaggcg
     3001 accgccctgc tcgacacgcc cgccccgcgc cttcagaccc tcctggacgc ggcggcggcg
     3061 ctgcgcgatc gcggtcacgg gcggcgcatc acgttctcgg ccaaggtctt catcccgctc
     3121 accacgctct gtcgcgacta ctgcggctac tgcaccttcc gcaaggatcc gggggagccc
     3181 ggtgccttca ccatgacgcc ggagcaggtg ctggcgctgg cgcacgcggg agcgcgcctg
     3241 ggcgccaagg aagccctctt ctccctgggc gacaagccgg aggccctctt cccggagcac
     3301 cgggcgttcc tgcgccgcat gggccaccgg accaccctcg gctacctccg cgaggtcagc
     3361 gcgcgggtgc tcgaggagac cgggatgctg ccgcacgcca accccggcct gatgagcgag
     3421 cgagacctgg ccgccctgcg cgaggtcaac gtcagcatgg ggatcatgct cgagagcacg
     3481 tccgagcgcc tgctcgcccc cggggccgcc cacgatcggg cccccgacaa ggtgcccgcg
     3541 cgacggctcc gcaccatcgc ccgggcgggg gcgctcggca tccccttcac caccggcatc
     3601 ctgatcggca tcggcgagac ccacgccgag cgcgtcgagt cgctcctcgc gatccgggat
     3661 ctgcacgagc ggcacggaca catccaggag gtcatcgtcc agaacttccg ggccaagccg
     3721 acgatcccga tgcgcgggtg gcccgatccg gggtccgttg atcttctccg gacagtcgcg
     3781 gtggcgcgcc tgctgctggg accggcgatg aacatccagg cgccgccgaa tctatccgcc
     3841 gacggctacg agcggcttgc cgccgccggc ctcaacgact gggggggcgt atcgcccctc
     3901 acccccgatc
//
LOCUS       DATMCV010000027         3917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_866335, whole genome shotgun sequence.
ACCESSION   DATMCV010000027 DATMCV010000000
VERSION     DATMCV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866335"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VNU03_00740"
     CDS             <1..322
                     /locus_tag="VNU03_00740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HWP72999.1"
                     /translation="LVIPREHVDTLGDLPRPLTAPLFDAAAAVARALEAGLGADGTFV
                     AINNRVSQSVPHLHVHVVPRRRGDGLRGFFWPRGRYESPAAMEETRARIAAALEGGAL
                     DARA"
     gene            complement(338..2125)
                     /locus_tag="VNU03_00745"
     CDS             complement(338..2125)
                     /locus_tag="VNU03_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWP73000.1"
                     /translation="MSEPIEDYALIGDGETAALVSRRGSIDWLCLPRFDSPACFAALL
                     GGPEHGRFSLAPRGGARSVRRRYRDSTLVLETEFETEGGAVTVVDCMPQREGPPVLIR
                     QVVGTRGTVAMRMELVMRMDYGSVVPWVRRSADGIHAVAGPEALALVTDLPLRGEGLT
                     TVADFEVAAGQRRSLTLAWHPSHLPAPPPLDPDAAVRDTEAWWRRWADACRFQGPDQD
                     AVVRSLVTIKALIYEPTGGIVAAPTTSLPEQLGGVRNWDYRICWVRDATFALYALVLG
                     GYLDEARAWRQWLLRALAGTPEQVQVMYGLAGERRLTEIELPWLPGYAGSAPVRIGNA
                     AHAQFQLDVWGEMLDAGYAAERAGLERDPEGSRVANKLMEHLESVWTEPDEGIWEVRG
                     PRRHFTHSKVMAWVAADRMVKLTEAAGGGEPDRWRALGAAIHADVCRRGFDPARNAFV
                     QAYGARELDASLLMIPLVGFLPPEDPRVRGTVAAIERELMVDGLVQRYTTESGVDGLP
                     PGEGVFLACSFWLVDNLVLQGRRDEARRLFDRLLGLRNDVGLLAEQYDPRARRQLGNF
                     PQALSHIALINSASNLSGGLRPAEHRAGR"
     gene            2302..>3917
                     /locus_tag="VNU03_00750"
     CDS             2302..>3917
                     /locus_tag="VNU03_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWP73001.1"
                     /translation="MAVDQIKRLYESMPGKLDEARRRFGRGLTLAEKILVAHADDFAK
                     QEWSRGKAQLRLRVDRVALQDATGQMALLQFMQAGKKQVAVPSTVHCDHLIRAESGAA
                     ADMERAIAENREVYDFLYSTSKKYGIGFWKPGAGIIHQVVLENYAFPGGLMIGADSHT
                     PNAGGLGMAAIGVGGADCGEAMAGLAWEVLDPKLIGIHLTGKLAGWSSPKDVITYLCG
                     LLTVKGGTNKIIEYFGPGAESISATGKGTICNMGAELGATTSLFHFDDRMAAYLRATD
                     RTDIARLAEQHRQHLVADPDVLRDPRKFYDEVVELNLSELEPHIVGPHSPDRARPISK
                     LAAEVKKEGWPAQIRQTLIGSCTNSSYEDMRRAAHIAMQALKAGIKAKTPFLVTPGSE
                     RIYQTIKRDGIMDTFQKIGGTVLANACGPCIGQWKRSDVGKEETNTIVSSFNRNFPGR
                     NDGSASTLSFLTSPEIVTAMAFGGTLEFDPVHGTIPGPDGKPFRFTAPEGEELPAQGF
                     AGGDAGFEAPAADGDNVQVAIAPNSERLQLL"
BASE COUNT          593 a         1412 c         1416 g          496 t
ORIGIN      
        1 cctggtgatc ccgcgcgagc acgtcgacac gctcggcgac ctgccccgcc cgctgaccgc
       61 gccactcttc gacgcggccg ccgcggtggc gcgcgcgctg gaggccgggc tcggcgccga
      121 cggcaccttc gtggcgatca acaaccgggt gagccagagc gtgccgcacc tgcacgtgca
      181 cgtggtgccg cggcggcgcg gcgacgggct gcgcggcttc ttctggcccc gcggccgcta
      241 cgagagtccg gccgcgatgg aggagacccg tgcccgcatc gccgccgcgc tcgaaggagg
      301 ggcgctcgac gcgcgggcgt gacccgcggt cgccggctca gcgcccggcg cggtgctccg
      361 cggggcgcag cccgcccgag aggttcgacg cgctgttgat cagcgcgatg tgcgagagcg
      421 cctgcgggaa gttgcccagc tggcggcggg cgcgcgggtc gtactgctcg gcgagcaggc
      481 ccacgtcgtt gcgcaggccg aggagccggt cgaagagccg ccgcgcctcg tcgcgccgcc
      541 cctgcagcac caggttgtcg accagccaga acgagcaggc caggaacacg ccctcgccgg
      601 gcggcaggcc gtccacgcct gactcggtgg tgtagcgctg cacgaggccg tcgaccatca
      661 gctcgcgctc gattgcggcc accgtcccgc gcacgcgcgg atcctccggc ggcaggaagc
      721 ccaccagcgg gatcatgagc aggctcgcgt ccagctcgcg cgcgccgtag gcctgcacga
      781 aggcgttgcg ggccggatcg aagccgcggc ggcagacgtc ggcgtggatg gccgcgccga
      841 gcgcgcgcca gcgatcgggc tcgcccccgc ccgccgcctc ggtcagcttg accatgcgat
      901 cggcggcgac ccacgccatc accttggagt gggtgaagtg ccggcggggc ccgcgcacct
      961 cccagatgcc ctcgtcgggc tccgtccaca ccgactcgag atgctccatc agcttgttgg
     1021 cgacgcgcga gccctccggg tcccgctcga gcccggcgcg ctcggccgca tagcccgcgt
     1081 cgagcatctc gccccagacg tcgagctgga actgggcgtg ggccgcgttg ccgatgcgta
     1141 ccggggccga gcccgcgtag ccgggcagcc agggcagctc gatctcggtg aggcggcgct
     1201 cgcccgcgag cccgtacatg acctgcacct gctcgggcgt gccggcgagc gcccggagca
     1261 gccactgccg ccaggcgcgc gcctcgtcga ggtagccgcc cagcacgagc gcgtagaggg
     1321 cgaaggtggc gtcccgcacc cagcagatgc ggtagtccca gttgcgcacc ccgcccagct
     1381 gctcgggcag cgaggtggtc ggcgcggcca cgatgccgcc ggtgggctcg tagatgagcg
     1441 ccttgatcgt gacgagcgag cgcaccaccg cgtcctgatc gggcccctgg aagcggcagg
     1501 cgtccgccca gcggcgccac caggcctccg tgtcgcgcac cgctgcgtcg gggtcgagcg
     1561 gtggcggcgc gggcaggtgg gagggatgcc aggcgagcgt cagcgagcgg cgctggccgg
     1621 ccgcgacctc gaagtcggcc accgtggtga gcccctcgcc gcgcaacggc aggtcggtga
     1681 cgagggcgag cgcctcgggc ccggccaccg cgtggatgcc gtccgcggag cgccgcaccc
     1741 aggggaccac ggagccatag tccatgcgca tcaccagctc catgcgcatc gccacggtcc
     1801 cccgggtgcc gacgacctgg cggatcagca ccggcgggcc ctcccgctgg ggcatgcaat
     1861 cgaccaccgt caccgcgccg ccctcggtct cgaactccgt ctccaggacc agggtgctgt
     1921 cgcggtaccg gcgacggacg gagcgggcgc cgccgcgcgg agccaggctg aaccggccgt
     1981 gctcggggcc gcccagcagg gccgcgaagc aggcggggga gtcgaagcgg ggcaggcaga
     2041 gccagtcgat ggagccccgg cgcgagacca gggccgcagt ctcgccgtct ccgatcagcg
     2101 cgtagtcctc gatgggctct gacatgggtt cgggcggggg cgttcccttg acctcgcccg
     2161 ggcgacatgt tagcgtaact cgtgatttta catggtcgcc tcctgcctag ccggtgggag
     2221 gcgatacgtg tgtgtgcggt ctgccgcggc ccactggaaa agcggcaccc gcccagcgcg
     2281 aaatagtcga ggaggcccac catggcggtt gaccagatca agcggctgta cgaatccatg
     2341 cccggcaagc tcgatgaggc gcgccggcgc ttcggccgcg ggctcaccct ggcggagaag
     2401 atcctggtcg cccacgccga cgactttgcg aagcaggagt ggagtcgcgg caaggcccag
     2461 ctgagactgc gggtcgatcg ggtggccctg caggacgcta ccggccagat ggccctgctc
     2521 cagttcatgc aggcgggcaa gaagcaggtg gcggtgccga gcacggtgca ctgcgaccac
     2581 ctgatccggg cggagagcgg cgcggcggcg gacatggagc gcgccatcgc ggagaaccgc
     2641 gaggtctacg acttcctgta ctcgacctcc aagaagtacg ggatcggctt ctggaagccg
     2701 ggcgccggca tcatccacca ggtcgtcctc gagaactacg ccttcccggg cggcctcatg
     2761 atcggcgccg actcccacac ccccaacgcg ggcggtctcg gcatggccgc catcggggtg
     2821 ggcggcgcgg actgcggcga ggcgatggcc gggctcgcct gggaggtgct cgatcccaag
     2881 ctgatcggca tccacctgac cggcaagctc gcgggctgga gctcgcccaa ggacgtgatc
     2941 acgtatctct gcgggctcct gaccgtgaag ggcggcacca acaagatcat cgagtacttc
     3001 gggccggggg cggagtcgat cagcgccacc ggcaagggca ccatctgcaa catgggcgcg
     3061 gagctggggg cgacgacctc gctcttccac ttcgacgacc ggatggccgc gtacctgcgg
     3121 gccaccgacc gcaccgacat cgcgcggctg gccgagcagc atcgccagca cctggtggcc
     3181 gatccggacg tgctgcgcga cccgcggaag ttctacgacg aggtggtcga gctcaacctc
     3241 tcggagctcg agccgcacat cgtgggcccg cactcgccgg accgggcgcg cccgatctcg
     3301 aagctcgcgg ccgaggtgaa gaaggaaggc tggccggccc agatccgcca gaccctcatc
     3361 ggctcctgca ccaactcctc ctacgaggac atgcggcggg ccgcccacat cgcgatgcag
     3421 gcgctcaagg ccggcatcaa ggccaagacc ccgttcctgg tgaccccggg ctcggagcgc
     3481 atctaccaga cgatcaagcg cgacggcatc atggacacct tccagaagat cggagggacc
     3541 gtgctcgcga acgcgtgcgg gccgtgcatc gggcagtgga agcgcagcga cgtgggcaag
     3601 gaggagacca acaccatcgt ctcctcgttc aaccggaact tccccggccg caacgacggc
     3661 agcgcctcca ccctgtcgtt cctgaccagc cccgagatcg tcaccgcgat ggccttcggg
     3721 ggcacgctcg agttcgaccc ggtccacggg acgattcccg gccccgacgg caagccgttc
     3781 cgcttcaccg cgccggaggg cgaggagctg cccgcccagg gcttcgcggg cggcgacgcg
     3841 gggttcgagg cgccggccgc cgacggcgac aacgtgcagg tcgcgatcgc gccgaacagc
     3901 gagcggctgc agctcct
//
LOCUS       DATMCV010000028         2286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_201243, whole genome shotgun sequence.
ACCESSION   DATMCV010000028 DATMCV010000000
VERSION     DATMCV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201243"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VNU03_00755"
     CDS             <1..198
                     /locus_tag="VNU03_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP73002.1"
                     /translation="AEKQFGFTAVTSATGATIRRLAGLAPRTLAVMHGSSFSGAAAPV
                     LESLAGFYDDLLQKGASTAGG"
     gene            231..1676
                     /locus_tag="VNU03_00760"
     CDS             231..1676
                     /locus_tag="VNU03_00760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWP73003.1"
                     /translation="MRRVALAVLLLVVAGLAIAAAVFRPGKAVRVATGLVSHTLCSET
                     FVAGLDPDQTFAETVRTMPGVRRLVRWLHYRVDRTAREVSTTLGGRFEMRAVYHDGVG
                     CIVALPAPAGPAPPLPSPASAGTPAAAPAPAGAADAPEAGPDIAGPAVVDPASEPLRA
                     ALDRVFAEPPGGPVRGTKAVVVVHDGRVVAERYAPGYGLETPMLSWSMNKSVVNALIG
                     VLVRQGRLAIGAPASVPAWRDPSDPRHAITVEQLMRMTSGLALDETNTGFDPSSRMLF
                     LEPDMAGFAESAALAAPPGTRYHYSSPSTLILSRIIRDAVGGRAEDVRRFAERELFAP
                     LGMRGVTLEFDATGTQVGSTYMYAMARDWARFGQLYAADGVAGGRRILPEGWVDYSAS
                     PTAGSRDGYAAGFFTNRGESEFARLRVRGGMPADSFFASGTQGQRIVVSPARRLVVVQ
                     LGRSQDWETFDIRGLIRLVADVDRALAGPAR"
     gene            complement(1673..>2286)
                     /gene="yidC"
                     /locus_tag="VNU03_00765"
     CDS             complement(1673..>2286)
                     /gene="yidC"
                     /locus_tag="VNU03_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWP73004.1"
                     /translation="LAVSVKVLLLPLAAVAERLQEQVNATQARLQPGLDAINAAHRGE
                     ERVRRTLALYRELGVHPLYTLKSLAGFLIQIPVFIAVFDMLAEDVDLYRAPFLWIPDL
                     SRPDALAALPGCVPFFGCALNLLPFLMSGVSLATTLRFRSPVLTPALVRRQRRNLASM
                     TGLFFLLFYTFPAGMVLYWTSTNAFQLVSQEIGRWRRGRRGAG"
BASE COUNT          287 a          855 c          843 g          301 t
ORIGIN      
        1 gccgagaagc agttcggctt caccgccgtc acctcggcga ccggcgccac catccgacgc
       61 ctggccgggc tcgccccgcg taccctggcc gtgatgcacg gctcgtcgtt ctccggcgcg
      121 gcggcgccgg tgctcgagtc gctcgccggc ttctacgacg acctgctcca gaaaggcgcg
      181 tcgaccgcgg gcggctagcc gcgcgcggtc gacgcatgcg gcttccaccg atgcgcaggg
      241 tggccctggc ggtcctgctc ctggtggtcg ccggcctggc gatcgcggcg gccgtgttcc
      301 gtcccggcaa ggcggtgcgg gtggccaccg gcctggtgag ccacaccctg tgctcggaga
      361 ccttcgtggc cgggctcgat cccgaccaga ccttcgccga gacggttcgc acgatgccgg
      421 gcgtccgccg cctcgtccgg tggctccact accgggtcga ccggacggcc cgggaagtca
      481 gcaccacgct cggcggccgc ttcgagatgc gggcggtcta ccacgacgga gtgggctgca
      541 tcgtcgccct tcccgcgccg gccgggcccg cgcctcccct gccctctccc gcgtcggcgg
      601 gcaccccggc ggccgcgccg gcgcccgcgg gcgcggcgga cgcgccggag gccggcccgg
      661 acatcgcggg ccccgcggtc gtcgatcccg cgagcgagcc gctgcgggcc gcgctcgacc
      721 gcgtcttcgc ggagcctccg ggcggaccgg ttcgcgggac caaggcggtg gtggtggtgc
      781 acgacggtcg cgtcgtcgcg gagcgctacg cgccgggcta cggcctcgag accccgatgc
      841 tctcctggtc catgaacaag tcggtggtga acgcgctcat cggcgtcctg gtgcggcagg
      901 ggcggctcgc gatcggagcg cccgcgtcgg tcccggcctg gcgcgatccg tccgacccgc
      961 gccacgcgat caccgtcgag cagctgatgc ggatgacgag cgggctcgcg ctcgacgaga
     1021 ccaacaccgg cttcgacccg tcctcgcgga tgctgttcct cgagccggac atggcgggct
     1081 tcgccgagtc ggccgcgctc gcggcgccgc ccggcacgcg ctaccactac agcagcccga
     1141 gcacgctgat cctctcccgc atcatccgag acgcggtcgg cggccgcgcg gaggacgtgc
     1201 ggcgcttcgc ggagcgcgag ctgttcgccc cgctcggcat gcgcggggtg accctcgagt
     1261 tcgatgcgac cggcacccag gtcggctcga cctacatgta cgcgatggcc cgggactggg
     1321 cgcgcttcgg ccagctgtac gccgccgacg gcgtcgcggg cgggcggcgg atcctccccg
     1381 agggctgggt cgactactcc gcctcgccga ccgcgggctc gcgggacggg tacgcggcgg
     1441 gcttcttcac caaccgcggc gagagcgagt tcgcccggct ccgcgtgcgc ggcggcatgc
     1501 ccgccgactc gttcttcgcc tccggcaccc agggtcagcg catcgtcgtc tctcccgcgc
     1561 gccggctcgt ggtggtccag ctcggccgct cgcaggactg ggagaccttc gacatccgcg
     1621 gcctgatccg cctggtcgcc gacgtcgacc gggcgctcgc cggccccgcc cgctagcccg
     1681 cgccccggcg cccccgccgc caccgcccga tctcctggct cacgagctgg aaggcgttgg
     1741 tcgaggtcca gtagaggacc atgccggcgg ggaacgtgta gaagagcagg aagaagagcc
     1801 cggtcatgga cgcgaggttc cggcgctgcc ggcggacgag cgccggggtg agcaccgggg
     1861 agcggaagcg gagcgtggtc gcgagcgaga cgccgctcat caggaacggc agcaggttga
     1921 gcgcgcagcc gaagaagggc acgcacccgg gcagcgcggc gagcgcgtcc gggcgcgaca
     1981 ggtccgggat ccagaggaac ggcgcgcggt agagatcgac gtcctccgcg agcatgtcga
     2041 agaccgcgat gaacaccggg atctggatca ggaagccggc caggctcttg agggtgtaga
     2101 gggggtggac gcccagctcg cgatagagcg cgagcgtgcg ccggacccgc tcctcccccc
     2161 ggtgagccgc gttgatcgcg tcgagcccgg gttgcagccg cgcctgcgtc gcattgacct
     2221 gctcctgcag ccgctccgcc accgcggcca gcggcagcag cagcaccttg accgacaccg
     2281 cgaggg
//
LOCUS       DATMCV010000029         3916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_600686, whole genome shotgun sequence.
ACCESSION   DATMCV010000029 DATMCV010000000
VERSION     DATMCV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600686"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1405
                     /locus_tag="VNU03_00770"
     CDS             335..1405
                     /locus_tag="VNU03_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73005.1"
                     /translation="MNCEDARCGLSALVDGPIGLTDWALLEAHLKQCAQCRQVEAHLR
                     QLAAASHPVTPPRAVLASLRKAMALARIGATCSTALVVRRRALLTVAARELTPRATAD
                     VIRTSLAIPLRSSFVAMANALEAAVRRTIRFRPSGQAVGIVLALAFTLYALQGTDGPQ
                     LLARPTPPPRTELGPTRVEPTQVESQRLEPARLEPTRLEPVQAEPAQAEPAQLAPSLP
                     APAPSVEKKTVSGAPPRANERRPAPSEAPPRAAALSPPGVSASEPVREEAVSATHVVG
                     RLSAKNPRAAQRDFIALLADVGGTELGRSQRVRFTAVEVVVPQSRYDEFADGLARIGS
                     WRVEAARFPLPDAVHMTIRVTE"
     gene            1413..2309
                     /locus_tag="VNU03_00775"
     CDS             1413..2309
                     /locus_tag="VNU03_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP73006.1"
                     /translation="MDPRVVRFEGFEGIHLVADVWGDDRDWPVLLMHGGGQTRHAWGN
                     TAQVLAGYGWRAVSLDLRGHGDSEWAVNGDYSFTAHAADCVAVVDQLGRPPVLVGASL
                     GGVTAMLAEGGSDRVVSSGLVIVDVTHRTNPAGVQRIRDFMSSGVRGFASLEEAADAI
                     AAYTPHRPKRMNPAGLMKVLRQKRDGRWYWHWDPRLLDHGRTEVPRRDFEALFQAAMR
                     NIHVPTLLVRGKLSDVVTEEGVQEFLTQIPGAKLVDVGGAAHMVAGDQNDAFSLAVVE
                     FLQRDVRPSLPIDGSARRGPAG"
     gene            complement(2326..2631)
                     /locus_tag="VNU03_00780"
     CDS             complement(2326..2631)
                     /locus_tag="VNU03_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73007.1"
                     /translation="MVIRITWGKLRAGAWDEFERTYRANVITKGKNVKGLRGRWLAQD
                     SADKDTGFAVSLWDNASDMQTYEQGALYREIMAPLQPFFVGEYKTSRCEVKYTDTAG"
     gene            complement(2625..3809)
                     /locus_tag="VNU03_00785"
     CDS             complement(2625..3809)
                     /locus_tag="VNU03_00785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP73008.1"
                     /translation="MITRFGSLFAGHVDLDDEGLEGTAANDRWLSDEHLATVFPKSEA
                     IAKLMDRTGYDIFWLAEHHFQREGYECIPNVLMLALHLCHLTRNIRIGCGFNIAPMWH
                     PIRMAEDFATVDWLTNGRVVFGVGRGYHTREVEAFGAPLLDQAANRELFEEQVDLIFT
                     SFNQRAFSHQGKHYTIPPRVPYRGYELEEITLVPRPKTLPVECWQPVVSASPRALDFM
                     AKHDIRGIIGGGAAPGGANEKVVHAFREARARAGREGEPGEGLCIGFSFHIADSVEQG
                     IKEATPFFEENIKMFAPLGFVPGLTPEQIEAVADPKRARQANLPTLRHAVKAGTWLIG
                     PPELITEQLMEIQHKYPGLEVVNVGQPVGTPQAVILEQLERFSAQVMPAFKSKAKEGR
                     PW"
BASE COUNT          559 a         1368 c         1346 g          643 t
ORIGIN      
        1 tccaggcagg tggttgcgag ggttcccaac gggttgcgag gccgcccgct tcgattgcgc
       61 ggtaagagat tacccaggga ttgggaagga gcgccctact ccgccccccg aaccatctga
      121 agaagttgcg cgcgtagacg agcggaggcc cgcaggagca ctctgggcgg ccgcctcgac
      181 cccggggcgc gtcgcgggcg cttgacgatt tccctgcgcg ggactactct cccgtcacgt
      241 agttgaccgt tcccgcggcc gcccttcgac gggccgactg gcagtggcga tgatcagagt
      301 cccggttgct gtggaagcgc cgccggccac tgccatgaat tgcgaagacg cccgttgcgg
      361 gctctctgct ctcgttgacg gcccgatcgg cctgaccgac tgggctctcc ttgaagcgca
      421 tctgaagcaa tgtgcgcaat gccggcaggt ggaagcgcac ctgcgacagc tggccgcggc
      481 cagtcatccg gtcacaccgc cgcgcgcggt gctggcctcc ctccgcaagg cgatggctct
      541 cgcccggatc ggagcgacct gttccaccgc cctggtcgtt cggcgccgcg cgctcctgac
      601 ggtcgccgcc cgcgagctta cgccgcgcgc caccgctgac gtgatccgca catcgctggc
      661 gatcccgctc aggtcatctt tcgtcgcgat ggccaacgcg ctcgaggccg ccgtccggcg
      721 gacgatccgc ttcaggccgt cgggacaagc cgtgggtatc gtgctggctc ttgcgttcac
      781 gctctacgcg ctgcagggga ccgacggacc tcagctgctc gcccgaccca cgccccctcc
      841 acgtaccgaa ctcgggccca cgcgagtcga gccgacgcag gtcgaatccc agcgcctcga
      901 gcccgcgcga ctggagccca cgcggctcga gcccgtgcag gccgagccgg cgcaggccga
      961 gcccgcgcag ctcgcgccct ccctgccggc accggcacca tccgtcgaga agaagaccgt
     1021 atcgggcgcg ccaccgcggg ccaacgagcg acgacccgct ccgtccgagg ctccgcctcg
     1081 ggcggccgca ctttccccgc ccggcgtgtc cgcatccgag ccggtccgcg aggaggcggt
     1141 ctcggcgacc catgtcgtcg ggcgactgtc ggcgaagaat ccgcgtgcgg cccagcgtga
     1201 tttcatcgcc ctgctcgccg atgtgggtgg aacggagctc ggccggagcc agcgggtcag
     1261 gttcacggcg gtcgaagtgg tcgtgccaca atcccgctac gacgagttcg ccgatgggct
     1321 cgcccgcatc ggctcctggc gggtggaagc cgcgcgcttc ccgctgcccg atgcggtcca
     1381 catgaccatt cgcgtgaccg agtagccgac gggtggatcc aagggtcgtc cggttcgagg
     1441 gattcgaggg gattcacctc gtcgccgacg tctggggaga cgatcgcgac tggcccgtgc
     1501 tgctcatgca cgggggcggt cagacgcgtc acgcgtgggg gaacaccgcg caggtcctcg
     1561 ccggctacgg gtggcgggcc gtgtccctcg atctccgcgg tcacggcgac agcgaatggg
     1621 ccgtcaacgg ggactactca ttcacggccc atgcggcgga ctgtgtggca gtggtcgacc
     1681 agctcggccg gcccccggtg ctggtcgggg cctccctcgg cggcgtgacg gccatgctcg
     1741 cggaaggcgg gtcggatcgc gtggtgtcga gcggtctcgt catcgtcgac gtcacgcatc
     1801 gtacgaatcc ggccggggtg cagcggatcc gcgacttcat gagctcgggc gtgcgcgggt
     1861 ttgccagcct cgaggaggcc gcggacgcca tcgcggccta tactccgcat cgtccgaagc
     1921 ggatgaaccc cgcgggtctc atgaaagtcc tgcgccagaa gcgggacggt cgctggtact
     1981 ggcactggga tccgcggctc ctcgaccacg gacgcacgga agttccgcgg cgagacttcg
     2041 aggctctctt ccaggcggcc atgcggaata tccacgtccc cacgctcctc gtgcgcggca
     2101 agctcagtga cgtggtgacc gaggaggggg tgcaggagtt cctcacgcag atccccggtg
     2161 cgaagctcgt cgacgtcgga ggcgccgccc acatggtggc gggcgaccag aacgatgcct
     2221 tctcactggc ggtcgtcgag tttctccagc gcgacgtccg tccgagcttg ccgatcgacg
     2281 ggagcgcgcg ccgcggcccc gccgggtgac cccggtcggc gacggctagc cggccgtgtc
     2341 cgtgtacttc acctcgcagc gggaggtctt gtactcgccg acgaagaagg gctgcagcgg
     2401 tgccatgatc tcccggtaga gcgcgccctg ttcgtaggtc tgcatatcgg acgcgttgtc
     2461 ccacaggctc accgcgaagc cggtgtcctt gtccgccgaa tcctgggcga gccagcggcc
     2521 gcggaggccc ttgacgttct tgcccttggt gatgacgttc gcccgatagg tgcgctcgaa
     2581 ctcgtcccac gcgccggcgc gcagcttgcc ccacgtaatc cggatcacca tggtcgtccc
     2641 tccttggcct tgctcttgaa ggccggcatg acctgggccg agaagcgctc gagctgctcg
     2701 aggatcaccg cctgcggcgt gcccaccggc tgaccgacgt tgacgacctc gagcccgggg
     2761 tacttgtgct ggatctccat gagctgctcg gtgatgagct ccggcggtcc gatcagccag
     2821 gtccccgcct tgaccgcgtg gcgcagggtc ggcaggttgg cctgacgcgc ccgcttcggg
     2881 tcggcgaccg cctcgatctg ctcgggggtg agcccgggca cgaagccgag cggggcgaac
     2941 atcttgatgt tctcctcgaa gaacggggtg gcctccttga tcccttgctc gacgctgtcg
     3001 gcgatgtgga acgagaaccc gatgcagagg ccctcgcccg gctcgccctc ccgtccggcg
     3061 cgggcgcggg cctcccggaa ggcgtggacc accttctcgt tggccccgcc gggcgccgcc
     3121 ccgccgccga tgatgccccg gatgtcgtgc ttcgccatga agtcgagcgc ccgcgggctc
     3181 gcgctgacca ccggctgcca gcactcgacc ggcagcgtct tcggccgggg caccagcgtg
     3241 atctcctcga gctcgtagcc ccggtaggga acgcgcggcg ggatcgtgta gtgcttgccc
     3301 tggtgcgaga aggcgcgctg gttgaacgat gtgaagatga gatcgacctg ctcctcgaac
     3361 agctcgcggt tggcggcctg atcgagcagg ggcgccccga acgcctcgac ctcgcgggtg
     3421 tggtagccgc gcccgacccc gaacacgacc cggccgttgg tcagccagtc gacggtggcg
     3481 aagtcctcgg ccatccggat cgggtgccac atcggcgcga tgttgaagcc gcagccgatc
     3541 cggatgttcc gggtgaggtg gcagagatgg agagccagca tcagcacgtt cgggatgcac
     3601 tcgtaccctt cgcgctggaa gtggtgctcg gccagccaga agatgtcgta gccggtccgg
     3661 tccatgagct tcgcgatggc ctcgctcttc ggaaacaccg tggccaggtg ctcgtcggac
     3721 agccagcgat cgttcgcggc cgttccctcg agcccttcgt cgtccagatc cacgtggccc
     3781 gcgaacaggc tgccgaacct cgtgatcatg cgcgcgctcc cgcgagcgcc ggggccggca
     3841 tcaggccgta gaaccgcccc gcgttctcgc agaccatctt gcgggtgacg tcgggcggca
     3901 agtccttgaa ctgctc
//
LOCUS       DATMCV010000030         7126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_401144, whole genome shotgun sequence.
ACCESSION   DATMCV010000030 DATMCV010000000
VERSION     DATMCV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401144"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VNU03_00790"
     CDS             <1..602
                     /locus_tag="VNU03_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWP73009.1"
                     /translation="RKAGVPRYVQMSALGARPDARARYHQTKWEAEEAVRASGLDWTI
                     FRPSIIFGKGDEFVSVLGRMIKRLPIVPVLGDGRYRLQPIPVEQVAEAFARALRTPAT
                     IRQTYEVAGPTPYAFVDLLDEIARALGRTRARKMHVPLGPVRLMTRLLEWLPFYPVSS
                     DQLTMLEEESVTDPSRFFADLALEPEPLSTGLRRMFHRP"
     gene            599..1063
                     /locus_tag="VNU03_00795"
     CDS             599..1063
                     /locus_tag="VNU03_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73010.1"
                     /translation="MTEWPHKGAFPHQHGDARGLLERIEAQLRERIEEAIEMAALKLM
                     VDTRAQAGRPAPESTSAADRTEFQGIARALLERLRERFLADLPADLRPRYQQAESAAT
                     DEAARPLAGQVFLARQLPDYWQRFERYQQEDAQERLADAAKPGWLRRLFGQS"
     gene            complement(1067..1339)
                     /locus_tag="VNU03_00800"
     CDS             complement(1067..1339)
                     /locus_tag="VNU03_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73011.1"
                     /translation="MSHFERLLQDDLNRLIDRIAVRAGEGTAAGLKSDLKAHIERAEE
                     RLTVLRAALLDGYAEWERAMDQCEDLWALAGLRREAPEEAAQLRAA"
     gene            1452..2381
                     /locus_tag="VNU03_00805"
     CDS             1452..2381
                     /locus_tag="VNU03_00805"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HWP73012.1"
                     /translation="MLHTWILLLLSLPPRPSSLRVRAWRRLRVLGAVPLKSGAYLLPD
                     SADRYEQFQWLAQEIQRDGGDATLFRAERIENMSEADVVRLFQEARNQDYAGLADRYR
                     KLLLKGRRPRLTDELARLAREMDRIGEIDFFEAPGRREVERAREAVEERTTAGRVRAA
                     GAAPPLDLAALRGRRWATRPRPHVDRIASAWLIKRFVDPEAAFVFAAPDQIPGDAIPF
                     DMAGVDFGHHGEDCTFETLLRRTGLRDRKLSILAEIVHEADLKDQKFAREEARGIDLT
                     MRALLSAIKDDHEALDHGLILFDGLYATIGERT"
     gene            2378..3547
                     /locus_tag="VNU03_00810"
     CDS             2378..3547
                     /locus_tag="VNU03_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWP73013.1"
                     /translation="MSDPRTTWGLVRYFLYLGSLGFGGPVALIGYMQRDLVERRAWFS
                     KNDYMKGLALSQLAPGPLAAQLAICLGYVHGRVAGATLIGLAFILPSFLMTLALGALY
                     VGYGGLGWMQAAFYGVGAAVIGIIVRSAWKLMRLTMGRDLLLWAVAGLMALVTAWTED
                     EVGILFILCGVVVLLVKAPPRRLLAWAGSTRGLPALGWPLVLLTGLAAPATLGVLGQI
                     LWFFTKAGAFVFGSGLAIVPFLYGGVVQEYRWLTDQQFLDAVAVAMLTPGPVVITVAF
                     IGYLVGGLAGGTLAALGVFLPTYLFVVVPYRWFDRFSENPQVKAFVGGVTAAAAGAIA
                     GACFVLGRRAIFDVPTALLALGAFGLLWRFKVPEPVLIAGAGAVGVLIFWMRGVS"
     gene            3544..4140
                     /locus_tag="VNU03_00815"
     CDS             3544..4140
                     /locus_tag="VNU03_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73014.1"
                     /translation="MKRTGLLAAAAALAVAAVTSAQPSGGWLTGQTGRGTARWARQGE
                     ALTYEGGAAFGWAVREGTSYRDGWVETRFKPMAGREDRAGGVVWRWRDGDNYYVARAN
                     ALEDNVVAYKVVRGHRTDLTPVGAAPGTYGVKVPVPTGAWHTLRVDFTGAQFEITYDG
                     RRLFAVRDDTFPDPGLVGIWSKADSITEFNQFRQEAAP"
     gene            4137..4553
                     /locus_tag="VNU03_00820"
     CDS             4137..4553
                     /locus_tag="VNU03_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73015.1"
                     /translation="MSSDRPVLFVCLHGAAKSVLAAADFRRMAAERGLDIPAVSAGTE
                     PDLAIAPGVLRALLAEGVDLSDQVPRRLTPADVTGAARVVAFGCDLGSAAPAGAPVEQ
                     WADVPAVSDDLPAARAVIRRHLERLVDECARSRSGR"
     gene            4564..5715
                     /locus_tag="VNU03_00825"
     CDS             4564..5715
                     /locus_tag="VNU03_00825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73016.1"
                     /translation="MTADGRLVFTAKTARTFCYGFLGVLFPVYLAEHGMGARQIGVAV
                     TLTLLASAAMTFAIRRPAERYGARVALLAQAGLIVVSALVFLVTEQPWLIVAAAMVGN
                     LAVGTGETGPFLTLEQVAVTRATPRERLTTTLSLYNLAGYGASALGAAAVGRAGASPR
                     VLFAVFLLGGLVQMAAYALMRPDPARPSGPRPAVAMPSRPLIHRLAALFALDSLAGGF
                     VLQSLIAYWFYTRFEMSLAALGWTFFGAQILSGLSLLLAARLAPRLGLVNTMVFSHLI
                     SNVLLIGVALAPSAGLAVTLLLARHLLSQMDVPTRQSFLMLAVQDHEREGAASLTNMS
                     RTLAQSVSPALTGWVMASVSMAAPFFLGGGLKILYDVMLYATIRNVETR"
     gene            complement(5735..6142)
                     /locus_tag="VNU03_00830"
     CDS             complement(5735..6142)
                     /locus_tag="VNU03_00830"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HWP73017.1"
                     /translation="MDPKLEEAQAPVDRARAVLIYDGTCGLCQGGVSWISRRAVRGHL
                     EFLPCQAAERRARYPWMDERACLQAMQLILPDGRVLAGDAAIPEILRRMRGWRWLAGA
                     FRLPGIEVLGPLLYGWVARHRYQISCMLGRPRR"
     gene            complement(6168..>7126)
                     /locus_tag="VNU03_00835"
     CDS             complement(6168..>7126)
                     /locus_tag="VNU03_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HWP73018.1"
                     /translation="MESHRSGVAQGLFLTSGVPGRAPRAVDRMLAAVEILRRREGFAG
                     YVHIKMLPGAERDQVIAATRLATRVSVNLEGPNDAVVRGLAREKDYSGDLLPTLMLAG
                     RLSREARLEGRPGIAAAGTTTQFVVGAQGERDREILGVVGSLERQGLLHHAHFSAFQP
                     VEGTPMEGLPPTPAAREWRLYQAEHLLRQYGFRADELVFGGDGNLALEHDPKTAWALA
                     HVERFPVALERAPYELLLRVPGIGPTAARRLVAERRRTLPRDAQDLRRLGVDATRAGY
                     FLTLRGRRVRATLPAEQLRLFPPGGHLTQGPWRTAVPPCAYR"
BASE COUNT          906 a         2678 c         2500 g         1042 t
ORIGIN      
        1 cgcgcaaggc gggcgtgccg cgctacgtgc agatgagcgc gctcggcgcg cgtccggacg
       61 cccgcgcccg ctatcaccag accaagtggg aagccgagga ggcggtgcgg gcgagcggtc
      121 tcgactggac catcttccgg ccgtcgatca tcttcgggaa gggcgacgag ttcgtctcgg
      181 tgctgggccg catgatcaag cggctaccca tcgtgccggt actcggggac gggcgctacc
      241 ggctccagcc gatcccggtc gagcaggtcg cggaggcctt cgcccgcgcg ctccggaccc
      301 cggccaccat ccggcagacg tacgaggtcg cgggccccac gccctacgcg ttcgtggacc
      361 tgctcgacga gatcgcccgc gccctcgggc gcacccgcgc ccgcaagatg cacgtgccgc
      421 tcggacccgt gcgcctgatg acccgcctgc tcgaatggct cccgttctac ccggtctcct
      481 cggatcagct caccatgctg gaggaagaga gcgtcaccga tccgtcccgc ttcttcgccg
      541 acctggcgct cgagcccgag ccgctgtcga ccgggctgcg gcggatgttc caccgaccat
      601 gaccgagtgg ccgcacaagg gcgcgttccc ccaccagcac ggcgacgcgc gcgggctgct
      661 cgagcgcatc gaggctcagc tccgcgagcg gatcgaggag gccatcgaga tggcggccct
      721 caagctcatg gtggacaccc gcgcgcaggc gggccggccg gcgcccgaga gcacgagcgc
      781 ggcggaccgc accgagttcc aggggatcgc gcgcgcgctg ctggaacggc tgcgcgagcg
      841 tttcctcgcc gacctgcccg ccgatttgcg gccgcgctac cagcaggccg agtcggcggc
      901 gaccgacgag gctgcgcgtc cgctggcggg ccaggtcttc ctcgcccgcc agctgccgga
      961 ctactggcag cgcttcgagc gctatcagca ggaggacgcc caggagcgac tggccgacgc
     1021 ggccaagcca ggctggctcc gccggctctt cggacagtcc tgacgtctag gcggctcgga
     1081 gctgggccgc ctcttccggc gcctcgcgcc ggagccccgc cagcgcccag aggtcctcgc
     1141 actggtccat cgcccgctcc cactcggcat agccgtccag gagcgccgcg cggagcaccg
     1201 tgaggcgctc ctcggcgcgc tcgatgtgcg ccttcagatc cgacttcaag cccgcggcgg
     1261 taccttcgcc cgcgcgcacg gcgatccggt cgatcaggcg gttcagatcg tcctgcagca
     1321 gtctctcgaa gtggctcatc gctgcacctc ctgtgggact catatactaa acacatgagg
     1381 aggtatcgag tcaagtcgac gtctcgatgc ccgttgacgg gacgcagcag cacgagtatc
     1441 gtgtgcaaca catgttacac acctggatcc tcctgttgct gagcctgccg ccgcgcccct
     1501 cgagcctgcg ggtgcgcgcc tggcgtcggc tccgggtgct gggcgcggtg ccgctcaaga
     1561 gcggagcgta cctgctcccc gactcggccg accgctacga gcagttccag tggctcgcgc
     1621 aggagatcca gcgtgacggg ggcgacgcca ccctgttccg cgccgagcgc atcgagaaca
     1681 tgtcggaggc cgacgtcgtg cgcctgttcc aggaggcccg caaccaggac tacgcgggcc
     1741 tcgcggatcg ctaccgcaag ctgctgctca agggacgccg gccgcggctc accgacgagc
     1801 tggcgcgcct ggcgcgggag atggaccgca tcggggagat cgacttcttc gaggcgcccg
     1861 ggcggcgcga ggtcgaacga gcccgcgaag cggtcgaaga gcgcaccacc gcgggccgcg
     1921 tgcgagcggc gggggcggcg cctccgctcg acctggccgc gctccggggc cgccggtggg
     1981 ccacccgccc gcggccgcac gtggaccgga tcgcgtccgc ctggctcatc aagcgcttcg
     2041 tggatccgga ggccgcgttc gtcttcgcgg cccccgacca gattccaggc gacgcgatcc
     2101 cgttcgacat ggcgggcgtc gacttcggcc atcacggcga ggactgcacc ttcgagaccc
     2161 tgctccggcg caccgggctg cgtgaccgca agctcagcat cctcgccgag atcgtccacg
     2221 aggccgacct gaaggatcag aagttcgcgc gcgaggaagc ccgcggcatc gatctgacga
     2281 tgcgggcctt gctgagcgcg atcaaggacg accacgaggc cctggaccac gggctcatcc
     2341 tgttcgacgg cctgtacgcg accatcggag agcggacatg agcgatccgc ggaccacctg
     2401 gggcctggtg cgctacttcc tctacctggg cagcctcggc tttggagggc cggtcgccct
     2461 catcggctac atgcagcgcg acctcgtcga gcggcgcgcc tggttcagca agaacgacta
     2521 catgaagggc ctggcgctct cccagctcgc gcccggtccc ctcgccgcgc agctggcgat
     2581 ctgcctcggc tacgtccacg ggcgggtcgc cggggccacc ctgatcgggc tcgcgttcat
     2641 cctcccttcc tttctcatga ccctggccct gggcgccctc tacgtgggct acggcggcct
     2701 cggctggatg caggccgcgt tctacggcgt cggcgcggcg gtgatcggca tcatcgtccg
     2761 ctcggcgtgg aagctcatgc ggctcaccat gggccgcgat ctcctgctgt gggccgtcgc
     2821 cggcctcatg gccctggtga ccgcgtggac ggaggacgag gtcgggatcc tcttcatcct
     2881 gtgcggcgtg gtcgtcctgc tcgtgaaggc gccgccgcgc cggctactcg cgtgggcggg
     2941 atcgacgcgc ggcctccccg cgctcggctg gcccctcgtc ctgctcaccg ggctggccgc
     3001 ccccgccacc ctcggcgtgc tcggccagat cctctggttc ttcaccaagg cgggggcgtt
     3061 cgtcttcggg agcggcctcg ccatcgtgcc cttcctgtac ggcggcgtcg tgcaggagta
     3121 ccggtggctc accgaccagc agttcctcga cgcggtcgcg gtggccatgc tcacgccggg
     3181 gccggtcgtg atcacggtgg ccttcatcgg ctacctcgtg ggcggcctcg cgggcgggac
     3241 gctcgccgcg ctcggcgtct tcctgccgac ctacctcttc gtcgtggtcc cgtatcgctg
     3301 gttcgaccgc ttcagcgaga acccgcaggt gaaggccttc gtcggcggcg tcaccgcggc
     3361 cgccgcgggc gccatcgccg gcgcctgctt cgtgctcggg cggcgggcca tcttcgacgt
     3421 gcccaccgcg ctgctggccc tcggcgcctt cggcctgctg tggcgcttca aggtcccgga
     3481 gccggtcctc atcgcgggcg ccggagccgt cggcgtcctg atcttctgga tgcggggcgt
     3541 gtcgtgaagc gcaccgggct cctcgccgcc gccgcggccc tggcggtggc cgcggtgacg
     3601 tcggcgcagc cgtcgggcgg ctggctcacc gggcagaccg gccgcggcac cgcgcgctgg
     3661 gcccgccagg gcgaggcact gacctacgag ggcggggccg cgttcgggtg ggcggtgcgc
     3721 gagggcacgt cgtatcgcga cggctgggtg gagacgcggt tcaagccgat ggcggggcgc
     3781 gaggatcggg ccggcggtgt ggtgtggaga tggcgcgacg gggacaacta ctatgtcgcc
     3841 cgcgcgaacg cgctcgagga caacgtcgtc gcctacaagg tcgtgcgggg ccaccgcacc
     3901 gatctcactc cggtgggtgc cgcgcccggc acctacggcg tcaaggtccc ggtaccgacc
     3961 ggcgcctggc acacgctccg cgtcgacttc acgggcgccc agttcgagat cacctacgac
     4021 ggccggcgcc tcttcgccgt ccgcgacgac accttccccg accccggcct cgtggggatc
     4081 tggtccaagg ccgacagcat caccgagttc aaccagttcc gacaggaggc cgcaccatga
     4141 gcagcgatcg cccggttctc ttcgtctgcc tgcacggcgc cgccaagagc gtcctcgccg
     4201 ccgccgactt ccgacgcatg gccgcggagc gcggcctcga catccctgcc gtctcggccg
     4261 gcaccgagcc cgacctcgcc attgcccccg gcgtgctgcg cgccctgctc gcggaaggcg
     4321 tggacctgag cgatcaggtg ccgcgacggc tcacgcctgc cgacgtgacc ggcgccgcgc
     4381 gggtcgtggc cttcgggtgc gatctgggct ccgcggctcc agccggggcc ccggtcgagc
     4441 agtgggcgga cgtgcccgcg gtgagcgacg acctgcccgc cgcccgcgcg gtgatccggc
     4501 gccatctcga gcgtctcgtc gacgaatgcg cccgttcccg gagcgggcgc tgagcgcgat
     4561 cgcgtgaccg cagacgggcg cctcgtcttc acggccaaga ccgcgcgcac cttctgctac
     4621 ggcttcctcg gcgtgctctt cccggtctac ctggccgagc acgggatggg cgcccgccag
     4681 atcggcgtcg cggtcaccct caccctgctc gcgagcgcgg ccatgacgtt cgcgatccgg
     4741 cgccccgccg agcgctacgg ggcgcgggtc gccctgctgg cccaggccgg cctgatcgtc
     4801 gtctccgcgc tcgtcttcct ggttaccgag cagccctggc tcatcgtggc cgcggccatg
     4861 gtcggcaacc tcgcggtggg gaccggcgag acgggcccgt tcctgacgct cgagcaggtc
     4921 gcggtcaccc gggcgacccc gcgggagcgc ctgaccacga ccctcagcct ctacaacctg
     4981 gccggctacg gcgcctccgc gctcggggcc gccgcggtcg gccgcgcggg cgcctccccc
     5041 cgcgtgctct tcgcggtgtt cctcctgggc ggcctcgtcc agatggcggc ctacgcgctc
     5101 atgcggccgg acccggcgcg cccgtccggc cctcggcctg ccgtcgcgat gccgtcccgc
     5161 ccgctcatcc accgcctcgc cgcgctcttc gcgctcgact cgctcgcggg cggcttcgtg
     5221 ctccagagcc tcatcgcgta ctggttctac acgcgcttcg agatgagcct ggccgcgctc
     5281 ggctggacgt tcttcggcgc gcagatcctg tccggcctgt ccctgctgct ggccgcgcgg
     5341 ctcgcgcccc ggctggggct ggtcaacacc atggtgttct cgcacctgat ctcgaacgtg
     5401 ctgctgatcg gggtggcgct ggccccgtcg gcgggactcg cggtcaccct gctgctcgcc
     5461 cgccacctgc tctcccagat ggacgtgccc acccgccaga gctttctcat gctggcggtg
     5521 caggatcacg agcgcgaggg ggccgcgagc ctcaccaaca tgagccgcac gctcgcgcag
     5581 tcggtgagcc ccgccctcac cggctgggtg atggcgagcg tgtcgatggc ggcgccgttc
     5641 ttcctgggcg ggggcctcaa gatcctctac gacgtgatgc tctacgcgac catccgcaac
     5701 gtggagacgc ggtgaggact gcggatcgcg tggatcagcg tcgcgggcgg ccgagcatgc
     5761 aggagatctg gtagcgatgg cgcgcgaccc agccgtagag cagcggcccc agcacctcga
     5821 tgccggggag gcggaacgcc cccgccagcc agcgccagcc ccgcatccgg cggaggatct
     5881 ccgggatcgc ggcgtcgccc gccaggaccc gcccgtccgg caggatgagc tgcatggcct
     5941 gcagacacgc ccgctcgtcc atccagggat agcgggcgcg ccgctcggcc gcctggcagg
     6001 gcaggaactc gaggtggccg cggacggcgc ggcgcgagat ccacgagacg ccaccctggc
     6061 acagcccgca ggtcccgtca tagatgagga cggcgcgagc gcggtcgacc ggggcctggg
     6121 cctcctcgag cttcgggtcc atcaggccgc cattgtactc ccgcgggtca gcggtaggca
     6181 cacgggggca ccgcggtccg ccagggcccc tgggtgaggt ggccgccggg cgggaacagg
     6241 cgaagctgct cggcgggcag cgtcgcgcgc acgcgccggc cgcgcagcgt gaggaagtag
     6301 ccggcccggg tcgcgtcgac gcccaggcgg cggagatcct gcgcgtcgcg cggcagcgtg
     6361 cgacggcgct ccgccaccag ccgccgggcc gcggtaggtc cgatccccgg cacccgcagc
     6421 agcagctcgt agggtgcgcg ctcgagcgcc accgggaacc gctccacatg ggccaacgcc
     6481 cacgccgtct tcggatcgtg ctcgagggcc aggttgccgt cacccccgaa gaccagctcg
     6541 tccgcccgga agccgtactg gcggaggagg tgctcggcct ggtagagccg ccactcgcgc
     6601 gcggccggcg tgggcgggag cccttccatc ggcgtgccct ccaccggctg gaacgcgctg
     6661 aagtgcgcgt gatgcagcag cccctggcgc tcgaggctgc ccacgacgcc caggatctcg
     6721 cgatcgcgct cgccctgggc gcccaccacg aactgcgtcg tcgtccccgc cgccgcgatg
     6781 ccgggccggc cctcgagccg cgcctcccgc gagagacggc cggccagcat gagggtcggg
     6841 agcagatcgc ccgaatagtc cttctcgcgg gcgaggccgc ggaccaccgc gtcgttgggc
     6901 ccctcgaggt tcaccgacac ccgcgtggcg agccgggtcg ccgcgatgac ctgatcgcgc
     6961 tccgcccccg gcagcatctt gatgtgcacg tagccggcga agccctcgcg tcgcctcagg
     7021 atctcgaccg ccgcgagcat gcggtcgacc gcccgcgggg cgcgccccgg cacccccgag
     7081 gtgaggaaga ggccctgggc caccccgctc cggtgcgact ccatga
//
LOCUS       DATMCV010000031         3001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_201306, whole genome shotgun sequence.
ACCESSION   DATMCV010000031 DATMCV010000000
VERSION     DATMCV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201306"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VNU03_00840"
     CDS             <1..602
                     /locus_tag="VNU03_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73019.1"
                     /translation="LAVACVALTAFTRTSVLAILLLVPLLLGVVAVLHLAATHPERRP
                     SARQFLWSGFLGATILLGAILLFLGVDARYGIEAALLLVVLPGYFVYKALVARDVMFV
                     ATTVLLIGAAVVSFLPPINLGPRNIVAYVAFFELARAFMRRADAAARRYFTLRAEQPD
                     PASWGVGAGTLKTMDWVFALCIRGDDEEVIHEATARAVA"
     gene            574..993
                     /locus_tag="VNU03_00845"
     CDS             574..993
                     /locus_tag="VNU03_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73020.1"
                     /translation="MRRRLERWREAAVAAVVLAGATAGWTPGVSAQGTPGTEAFNIVG
                     LRMSKVTLYRDCSMDKGVPFSREDLEKRKPWRATNDAKTAVYYWVKMDGGDYCVKAFA
                     VETDRVVPVVKDEECKTRVAGRPPKTGAVRGVGESCN"
     gene            1005..2468
                     /locus_tag="VNU03_00850"
     CDS             1005..2468
                     /locus_tag="VNU03_00850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWP73021.1"
                     /translation="MRLPWVLAGLLAGGCAGGAGGDGTTPASWTPSWLQGKGGSTTTV
                     LARLKAGPYEASSLAVGEEKDLAQHRGDALGFVRSAALEQYLNETRRRLLTASGRTGV
                     PGRVVILANPGFEAFSSPDGNVYVAMGLLDSLESSDEVAAILAHELSHVLLAHHSSDL
                     LGDVQRKGQALYELGMGAKTALAGQRTVSKGDARNMTNVQLATDATDKLVLPAWSRGQ
                     EREADLLGMDLLVESHVAGPAMMSMLEKLQAWEQANKESENAFSERLQQASQRNVNEA
                     VGVVYQKLLGTVSVNHPKTSERIEDAAQYFERFYGSRPPVEPQAGPLKAVRARPEVAQ
                     VLSSYKRAFQARRLLDQGKPQEAYTAARASVGGRTDAYPNWVLAHSAAAIGRQTEAID
                     ALRRAVGSPEPVPLVYEDLIFAYERTGNVPVALQWTDQASKVFAGAARFKPHKIRLLR
                     KAGRAAEAQTLTVDCALNGTTELKRACQQANQTPAGR"
     gene            complement(2601..>3001)
                     /locus_tag="VNU03_00855"
     CDS             complement(2601..>3001)
                     /locus_tag="VNU03_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73022.1"
                     /translation="HVPNRVTGRKYPYLDAVLETARRTWPGPAVFLGDTNSGRIGLDE
                     EVPAFNRIEDGWMRELHEAGWIDAFRHLRGDARAYTWYSPNGGNGFRIDQAFLNRALR
                     PRLESFRYDWGRRDRRSASDHAAMLLDLTG"
BASE COUNT          427 a         1035 c         1108 g          431 t
ORIGIN      
        1 ccctcgcggt ggcgtgcgtg gccctcaccg cgttcacccg gacgagcgtg ctcgcgatcc
       61 tgctcctggt gccgctgctg ctgggggtgg tggcggtgct gcacctggcc gcgacgcatc
      121 ccgagcgacg gccctccgcg cgccagttcc tctggtcggg gttcctgggg gcgacgatcc
      181 tgctcggggc gatcttgcta ttcctgggag tcgacgcgcg gtacggcatc gaggccgcgc
      241 tcctgctggt ggtgctcccg ggctacttcg tctacaaggc cctggtcgcg cgcgacgtga
      301 tgttcgtggc caccacggtt ctattaatcg gggccgcggt ggtgagcttc ctgcccccga
      361 tcaacctggg cccgcgcaac atcgtggcct acgtggcgtt cttcgagctg gcgcgcgcgt
      421 tcatgcggcg cgccgacgcg gcggcccggc ggtacttcac cctgcgggcc gagcagcccg
      481 atccggcgag ctggggagtc ggcgccggca ccctgaagac gatggactgg gtcttcgccc
      541 tctgcatcag aggcgacgac gaggaggtca ttcatgaggc gacggctcga gcggtggcgt
      601 gaggcggcgg tggcggcggt ggtcctggcg ggtgcgaccg ccgggtggac gcccggggtg
      661 tcggcgcagg ggacgcccgg cacggaggcg ttcaacatcg tcgggctccg catgagcaag
      721 gtcacgctct accgcgactg cagcatggac aagggcgtgc cgttctcgcg ggaggacctc
      781 gagaagcgca agccgtggcg ggcgaccaac gacgccaaga ccgcggtcta ctactgggtg
      841 aagatggacg gcggggacta ctgcgtgaag gccttcgcgg tcgagaccga ccgggtggtc
      901 ccggtggtca aggacgagga atgcaagacc cgcgtggccg gccgtccgcc caagaccggc
      961 gcggtgcgcg gagtgggcga gagctgcaat tgaggcgtcc cgggatgcgc ctgccctggg
     1021 tgctggccgg actgctcgcg ggcggctgcg cgggcggggc ggggggtgac ggcacgaccc
     1081 cggcctcgtg gacgccctcc tggctccagg gcaagggcgg cagcaccacc accgtcctgg
     1141 cccggctcaa ggccggaccc tacgaggcgt cgagcctcgc ggtgggcgag gagaaggatc
     1201 tcgcccagca tcgcggcgac gcgctcggct tcgtgcgctc ggccgcgctc gagcagtacc
     1261 tgaacgagac ccggcgccgc ctgctgaccg cctcgggccg gaccggggtg ccggggcggg
     1321 tggtgatcct ggccaacccc ggattcgagg cgttctcctc gcccgacggc aacgtctacg
     1381 tggccatggg cctcctcgac tcgctcgaga gctcggacga ggtcgcggcc atcctcgccc
     1441 acgagctctc gcacgtgctg ctcgcccatc acagctcgga cctcctcggc gacgtgcagc
     1501 gcaagggcca ggcgctctac gagctcggga tgggcgccaa gaccgcgctc gcgggccagc
     1561 gcaccgtctc caagggagac gcgcggaaca tgaccaatgt gcagctcgcc accgacgcga
     1621 ccgacaagct ggtgttgccg gcctggagcc gggggcagga gcgcgaggcg gatctgctcg
     1681 gcatggacct gctcgtggag agccacgtgg ccgggcccgc catgatgagc atgctcgaga
     1741 agctccaggc gtgggagcag gcgaacaagg aatccgagaa cgccttctcc gagcgcctcc
     1801 agcaggcctc gcagcggaac gtcaacgagg cggtgggggt tgtctaccag aagcttctcg
     1861 gcacggtctc ggtgaatcat cccaagacct ccgagcggat cgaggatgcc gcccagtact
     1921 tcgagcgctt ctacggcagt cgcccgccgg tggagccgca ggccggaccc ttgaaggcag
     1981 tgcgggcccg ccccgaggtg gcccaggtgc tgtcgagcta caagcgggcc ttccaggccc
     2041 gccgcctgct cgatcagggc aagccccagg aggcgtacac cgcggcccgg gcctcggtgg
     2101 gcgggcgcac cgacgcctac cccaactggg tgctggccca ctcggcggcg gcgatcgggc
     2161 ggcagaccga ggcgatcgac gccctgcgcc gcgcggtcgg ctcgcccgag ccggttcctc
     2221 tggtctacga ggacctgatc ttcgcctacg agcggacggg caatgtcccg gtcgcgctcc
     2281 agtggaccga tcaggcctcg aaggtcttcg cgggcgcggc gcgcttcaag ccccacaaga
     2341 tccggctcct gcgcaaggcc gggcgggcgg ccgaagcgca gaccctcacg gtggactgcg
     2401 cgctcaacgg gaccaccgag ctcaagcgcg cgtgccagca ggcgaatcaa acgccggcgg
     2461 gtcgataagg acccatctgc ttcgttgacg ccctcggccg cacgctcgac gtacagggag
     2521 tacgcctcgc gtgcggcctt cgggcgtcgc ctcgcatctg ggcccctctc gagccgccgg
     2581 cgagcgtggg aggccgatgg tcagccggtg aggtcgagga gcatcgccgc gtggtcgctg
     2641 gcggagcgac ggtcgcgtcg gccccagtcg tagcggaagc tctccaggcg cggccgcagc
     2701 gcgcggttca ggaacgcctg gtcgatccgg aacccgttgc ccccgttcgg cgagtaccac
     2761 gtgtaggccc gcgcgtcccc gcgcaggtgc cggaaggcgt cgatccaccc cgcctcgtgg
     2821 agctcgcgca tccagccgtc ctcgatccgg ttgaacgcgg gcacctcctc gtcgagcccg
     2881 atccggcccg agttggtgtc gcccaggaaa acggcgggcc cgggccaggt ccggcgcgcg
     2941 gtctcgagca ccgcgtcgag gtaggggtac ttgcgcccgg tcacccggtt ggggacgtgc
     3001 a
//
LOCUS       DATMCV010000032         2463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_401236, whole genome shotgun sequence.
ACCESSION   DATMCV010000032 DATMCV010000000
VERSION     DATMCV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401236"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2463)
                     /locus_tag="VNU03_00860"
     CDS             complement(<1..>2463)
                     /locus_tag="VNU03_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP73023.1"
                     /translation="RLGEGTTGRAAELREAVWTSDILGDPAVSLSPETRAVVEREGYR
                     AVLSVPLVSKGEVLGALSAYWWETHTPTASEISVMSALAGQAAVALDNARLFAQERDR
                     KASLSSLLEINKKIGALAAPETLLASIAEEAARLLEVDNAGFRLLDGDDLVLAGLFGT
                     AAQTMVRPRIRVGESLSGRVVGSGHALMCELEGAEVVTEHRTASERLGYTHYLGVPLL
                     VGERPSGVLVFRGRRPFTAREQELAEIFAGQAAIAIDHSRLYREASAQAERMRAVAEL
                     GRMLVSTLDEARVLELVATHARETLDMPDLAIWLRDGETEFLRFAVGRGQFSGPLTDR
                     AQPLRMDEGVVGRVLDDKKPIWTADVLADPRIRLSPESRRWIEEVGGRAILAVPLIRE
                     HLQGALVAYRHAGERFTEREIEYLSVFANQVAAALENARLYEALDVRAARLRSLTRLA
                     HIVSSSLDMDEVLRAIAQAAGELIGVPLATFWVADEVARKLAVRAVSDPLGLGLPHQE
                     LTFGESAAGWVAQHREPLTIADVQQDARFASKEWAASRGMPSLLAVPIIVQDSLLGVL
                     TLNGRTAIELGPDDRQILDSFVAQAGVAIRNAGLYGETRGRLEESRALLEVAEILNST
                     LDSKRLLREVAIKIAQVCRVDRCSIQRWMDGRVVPLMSQYADGRSDPELWARYRSLAA
                     SLPSNPPLHMRAVETRRPVIIPDVATSDLAPPQWIEAFGVKSTMAVPLIQQDRVIGVM
                     LVDHVDRATAFEPWQVDLAMAIAGQLALALSNAGLYAQVQDRLRETTALLAVGHALSQ
                     PGSAQTAMRAVAREVGTAFGADM"
BASE COUNT          331 a          926 c          887 g          319 t
ORIGIN      
        1 catgtcggcg ccgaaggcgg tccccacctc acgggcgacc gcgcgcatcg cggtctgggc
       61 cgatccgggc tgcgagagcg cgtgcccgac cgcgaggagc gcggtggtct cgcgcagccg
      121 gtcctgcacc tgggcgtaga ggcccgcgtt ggacagggcg agggcgagct ggcccgcgat
      181 ggccatcgcc aggtcgacct gccagggctc gaaggcggtc gcgcggtcca cgtggtccac
      241 gagcatgacg ccgatgaccc ggtcctgctg gatcagcggc accgccatgg tcgacttgac
      301 gccgaaggcc tcgatccact ggggcggcgc cagatcgctc gtggcgacgt cggggatgat
      361 caccggccgg cgcgtctcca ccgcgcgcat gtggagcggc gggttcgagg gcagcgacgc
      421 ggcgaggctg cggtagcgcg cccacaactc cggatccgag cgcccgtccg cgtactgcga
      481 catgaggggc acgacccggc cgtccatcca gcgctggatc gagcagcggt cgacccggca
      541 cacctgggcg atcttgatcg cgacctcgcg aagcagccgc ttggagtcca gcgtggagtt
      601 caggatctcg gcgacctcga ggagggcccg gctctcctcc aggcggccac gggtctcccc
      661 gtagagcccc gcgttgcgga tggccacgcc ggcctgggcc acgaagctgt cgaggatctg
      721 gcggtcgtcg ggcccgagct cgatcgcggt gcggccgttc agggtgagca cgcccagcag
      781 cgagtcctgc acgatgatcg ggaccgcgag caggctcggc attcctcggc tcgccgccca
      841 ctccttgctc gcgaagcgcg cgtcctgctg cacatccgcg atggtgagcg gctcgcggtg
      901 ctgggccacc cagcccgccg cgctctcccc gaaggtcagc tcctggtggg gcaggcccag
      961 gccgagcggg tcggagaccg cccgcaccgc cagcttccgc gccacctcgt ccgcgaccca
     1021 gaaggtcgcg agcggcaccc cgatcagctc gccggcggcc tgcgcgatcg cgcgcagcac
     1081 ctcgtccatg tcgagcgagg acgacacgat gtgggccagc cgcgtgaggc tgcgcaggcg
     1141 ggccgcacgc acgtcgagcg cctcgtagag gcgcgcgttc tccagcgcgg ccgcgacctg
     1201 gttggcgaag accgagaggt actcgatctc ccgctcggtg aaccgctcgc ccgcgtggcg
     1261 gtacgcgacc agcgcgccct ggaggtgctc gcggatgagc ggcaccgcca ggatcgcgcg
     1321 gccgccgacc tcctcgatcc agcggcgcga ctcggggctc aggcggatcc gcggatccgc
     1381 gagcacgtcg gcggtccaga tcggcttctt gtcgtcgagg acccggccca ccacgccctc
     1441 gtccatccgg agcggctggg cccggtcggt gagcggcccc gagaactgcc cccgccccac
     1501 cgcgaagcgc aggaactcgg tctcgccgtc gcggagccag atcgcgaggt cgggcatgtc
     1561 gagcgtctcc cgggcgtggg tggcgaccag ctccagcacc cgcgcctcgt cgagcgtcga
     1621 gaccagcatc cggcccagct cggcgaccgc ccgcatgcgc tccgcctgcg cggaggcctc
     1681 gcggtacagg cgcgagtggt cgatcgcgat cgcggcctgc cccgcgaaga tctcggccag
     1741 ctcctgctcg cgcgcggtga agggccggcg cccgcggaag acgagcaccc cgctgggccg
     1801 ctcgccgacc aggagcggca cccccaggta gtgcgtgtag cccaggcgct cgctggccgt
     1861 gcggtgctcg gtcaccacct cggccccctc gagctcgcac atgagggcgt ggccggagcc
     1921 cacgacccgg ccgctgaggc tctcccccac ccggatccgg ggccggacca tggtctgggc
     1981 cgcggtcccg aacaagcctg cgagcaccag gtcgtcgccg tcgaggagcc ggaaccccgc
     2041 gttgtccacc tcgagcagcc gcgccgcctc ctccgcgatc gaggcgagca gggtctcggg
     2101 cgcggcgagc gcgccgatct tcttgttgat ctcgagcagg gaggagagcg aggccttgcg
     2161 gtcgcgctcc tgggcgaaca ggcgcgcgtt gtcgagggcc accgcggcct gcccggccag
     2221 cgcgctcatg accgagatct cgctcgcggt tggcgtgtgg gtctcccacc agtaggcgga
     2281 gagcgcgccc agcacctcgc ccttgctgac caggggcacc gacagcaccg cgcggtagcc
     2341 ctcgcgctcg accaccgccc gcgtctccgg gctcagcgag accgccggat cgcccaggat
     2401 gtcgctggtc cataccgcct cgcgcagctc cgcggcgcgc ccggtggtgc cctcgccgag
     2461 ccg
//
LOCUS       DATMCV010000033         3917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_666432, whole genome shotgun sequence.
ACCESSION   DATMCV010000033 DATMCV010000000
VERSION     DATMCV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666432"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VNU03_00865"
     CDS             <1..265
                     /locus_tag="VNU03_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWP73024.1"
                     /translation="GLPDLAAVRAVCSAVSKPVNFMVGIKGKSFTVTELTAAGVKRIS
                     LATSLYRSAMSGLLDAAREVKDAGTFGYVERGVPSPDLAAFME"
     gene            complement(477..1514)
                     /locus_tag="VNU03_00870"
     CDS             complement(477..1514)
                     /locus_tag="VNU03_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HWP73025.1"
                     /translation="MVLDSTVRSLRVRLLLPLSLVVVVIVIGTAGYHVLWLDVGGTWM
                     DALFMTVTTITTIGYGEIKPLTTAGRLFTMGLAITGIGSMFYTLGVVMEYLVSVRLAD
                     PMGRRRMERRIRELAGHVIVAGMGRVGQQAALELHGAGTAFVVVDTDEPAVRHAAERG
                     YLAVVGDATQDEVLEQVGIRRARGLIVTTGEDATNIYIVLSARVLSPDLHIVARATGE
                     AGVTKLARAGANRAVSPYAIGGHRLAHLMLSPAVVDFFETALRRGNDTLAIEDLAMPA
                     DSPCVGQTLDTLNIRRATGVTVLAILRDGNPMVSPPGDLALAAGDHLLALGTSDQLRR
                     LEQLIGSGVAA"
     gene            1565..2677
                     /gene="ald"
                     /locus_tag="VNU03_00875"
     CDS             1565..2677
                     /gene="ald"
                     /locus_tag="VNU03_00875"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HWP73026.1"
                     /translation="MIIGVPREIKPGEQRVALTPAGVRAFVEARHQVVVERGAGQGSS
                     IRDEEYARERATLAGVDEVWTRSQMILKVKEPLPEEYARMRPGQVLFTYLHLAAVPEL
                     ARALQAADVVAIAYETVQRPDGSLPLLAPMSEVAGRLSVQEGAFYLAKAHGGRGILLA
                     GVPGVPPGNVVVVGAGVVGVNAARIALGLGADVSILDVNLDRLRAVDDLFHGRVITLM
                     SNSYNLDQVLRRADLLVGAVLITGARAPVLVTKEMVGAMKEGSVIVDVAVDQGGSVET
                     IHPTTLLDPIYLVSGVVHYGVANMPALVPRTSTFALTNATLPYALALAGEGLEPALRR
                     SPVLAHGVNIWRGRIVHPAVAEALGEPATSLETLLG"
     gene            2704..2886
                     /locus_tag="VNU03_00880"
     CDS             2704..2886
                     /locus_tag="VNU03_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73027.1"
                     /translation="MSGKVLNLVGVVWVVGALVTLGYFAVRDIQILPVLQRGMAAEPG
                     PVKHLIPARLETPAPK"
     gene            3031..>3917
                     /locus_tag="VNU03_00885"
     CDS             3031..>3917
                     /locus_tag="VNU03_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HWP73028.1"
                     /translation="MRQPLGKASALARLAVIGLVIAAVGGTFLYLGGWLTPRDLTPAR
                     LTDAFEHVDGLHAGFRRNHAKGVGVSGIFESNGNGVRLSEAVVFRPGRVPVIGRFSLG
                     GGQPYVGDTPDAVRGLGLQFSLPDGELWRTAMVNLPVFPVRTPEAFYQLLLASQPDPG
                     TGKPDPARMGAFLARHPETVKAFTVIKSQPVSSGFGDSTFHGLNAFRFRNRAGDSTPV
                     RWLMSPMQPFEPAGPGPLQGKNSLFDALITAIHRQPLRWRLIVIVGQPGDPTNDASVA
                     WPADRQQVDVGTVMLDRVE"
BASE COUNT          548 a         1347 c         1420 g          602 t
ORIGIN      
        1 gggcctgccc gacctggccg cggtgcgcgc ggtgtgcagc gcggtctcga agccggtcaa
       61 cttcatggtg gggatcaagg gcaagtcctt cacggtgacc gagctgaccg cggctggcgt
      121 caagcggatc agcctcgcca cgtcgctcta tcgctcggcg atgagcggcc tgctcgacgc
      181 ggcccgcgag gtgaaggacg cgggcacatt cggctacgtg gagcgcggcg tgccctcgcc
      241 ggatctcgcc gcgttcatgg agtgacgcgc cggcggctca gggcccccgg aggtcacagt
      301 agccgccgcg gaggtcgtca ggccgccacc agccccggtg ccgatcgcac tcggctttga
      361 gctcgccctg ggtgtaggtc ggcgggacgg gagctcccgt cccgcagccg agcgcatacg
      421 tggcggctcc ggccgcgagg catagcgcgc gcatccagcc cggggtcctg gtcatctcag
      481 gccgccacgc ccgagccgat caactgctcc agtcgccgca gctgatcgct ggtgcccagc
      541 gcgaggaggt gatcgccggc cgccagcgcc aggtcgccgg gcgggctcac catcggattg
      601 ccgtcgcgca ggatagccag cacggtgacg ccggtggccc ggcggatgtt gagggtgtcg
      661 agcgtctggc cgacacacgg gctgtccgcc ggcatcgcca ggtcctcgat ggcgagggtg
      721 tcgttgccgc gccggagcgc ggtctcgaag aaatccacca ccgccgggct cagcatcagg
      781 tgcgcgaggc ggtggccgcc gatcgcgtag gggctgaccg cccggttggc cccggcgcgg
      841 gcgagcttgg tgacgcccgc ctcaccggtc gcgcgggcca cgatgtgcag gtcgggactg
      901 agcacgcgcg ccgagagcac gatgtagatg ttggtggcgt cctcgccggt ggtgacgatg
      961 aggccgcggg cgcgccttat gccgacctgc tcgagcacct cgtcctgggt cgcgtcaccc
     1021 accaccgcca ggtacccgcg ctccgccgcg tgccggaccg cgggctcgtc ggtgtccacg
     1081 accacgaagg cggtccccgc cccgtggagc tccagcgcgg cctgttggcc cacccggccc
     1141 atccccgcca cgatcacgtg cccggcgagc tcccggattc gacgctccat tcgacgcctc
     1201 cccatgggat cggccagacg gacgctgacc aggtactcca tgacgacacc cagcgtgtag
     1261 aacatgctgc cgatgcccgt gatggcgagc cccatcgtga agaggcgacc ggcggtggtg
     1321 agcggcttga tctcgccgta gccgatggtg gtgatcgtgg tcacggtcat gaacagggcg
     1381 tccatccacg tcccgcccac gtcgagccac aggacgtgat agccggcggt gccgatcacg
     1441 atgacgacga cgacgaggga cagcggcagc aggagccgca cccggaggga ccgcaccgtc
     1501 gagtccagca ccatcggcgc tacgctagca tagccgcgcg ttggtatggc aggatgcagg
     1561 cagcgtgatc atcggcgttc cgcgggagat caagccgggc gagcagcggg tggccctgac
     1621 gcccgccggc gtccgggcct tcgtcgaggc acggcaccag gtggtggtcg agcggggcgc
     1681 cgggcagggc agcagcatcc gcgacgagga gtatgcgcgg gagcgcgcca cgctggccgg
     1741 ggtcgacgag gtctggaccc gatcccagat gatcctcaag gtgaaggagc cgctgccgga
     1801 ggagtacgcc cggatgcggc ccggccaggt gctcttcacc tatctgcacc ttgccgcggt
     1861 gccggagctg gcgcgggccc tccaggccgc cgacgtcgtg gcgatcgcct acgagacggt
     1921 gcagcggccc gacggcagcc tgcccctgct cgcgccgatg agcgaggtgg ccgggcgcct
     1981 gtcggtgcag gagggcgcgt tctacctggc caaggcccat ggcggccggg ggatcctgct
     2041 cgccggggtg ccgggcgtgc cgcccggcaa cgtggtggtg gtgggcgcgg gggtcgtcgg
     2101 ggtgaacgcg gcgcgcatcg cgctcggcct cggcgccgac gtctcgatcc tcgacgtgaa
     2161 cctcgatcgc ctgcgtgcgg tggacgatct cttccacggc cgggtgatca cgctcatgtc
     2221 caacagctac aatctcgacc aggtgctgcg gcgcgccgat ctgctggtgg gcgcggtgct
     2281 catcaccggc gcgcgggcgc cggtgctcgt caccaaggag atggtggggg ccatgaagga
     2341 gggctcggtg atcgtggacg tcgcggtcga ccagggcggc agcgtggaga cgatccaccc
     2401 gaccacgctg ctcgatccca tctacctggt ctcgggcgtg gtgcactacg gcgtggccaa
     2461 catgccggcc ctggtgcccc gcacgtccac gttcgccttg accaacgcga ccttgccgta
     2521 cgcgctggcg ctggccggcg aggggctcga gcccgcgctg cgccgcagtc ccgtgcttgc
     2581 gcacggcgtg aacatctggc gcgggcgcat cgtgcacccg gcggtggccg aggcgctcgg
     2641 cgagcccgcg acgtcactcg agacgctgct cggctgaggg ccgggcggca aggaggagag
     2701 agcatgagcg gcaaggtcct gaacctcgtc ggggtggtgt gggtcgtcgg cgccctggtc
     2761 accctgggct acttcgcggt gcgcgacatc cagatcctgc ccgtgctgca gcgtggaatg
     2821 gccgcggagc cggggccggt caagcacctg atcccggccc gcctggagac ccccgcgccg
     2881 aagtagccct tcgggcttcg agtttagggg ccggcgggct gctttcgtat tcttgccgga
     2941 atagtcgcgc cgtccagtct cccgctcgag gggagccatc aaacgagcgt gtgcccctcc
     3001 gagcgtctgg gcgtgagcgt tccgagagcc gtgcggcagc ccctcggcaa ggccagcgcg
     3061 ctggccaggc tggcggtgat cggcctcgtg attgcggcgg tcggcggcac gttcctctac
     3121 ctcggtgggt ggctcactcc ccgtgatctg acgcccgcgc ggctcaccga tgcgttcgag
     3181 cacgtcgacg gactccacgc cggcttccgt cgcaaccacg ccaagggcgt gggcgtgtcg
     3241 gggatcttcg aaagtaacgg gaacggtgtg cgtctctcgg aggcggtcgt ctttcgaccc
     3301 gggcgggttc cagtgatcgg gcgcttttcc ctggggggcg gccagcccta tgtcggcgac
     3361 acgccggacg cggtgcgggg cctgggactg cagttctccc ttcccgacgg ggagctgtgg
     3421 cgaaccgcga tggtcaacct gccggtgttc ccggtccgca ctcccgaagc cttctatcag
     3481 ctgttgctcg cgtcgcagcc ggaccccggc accggcaagc cggatccggc ccggatggga
     3541 gcctttctcg cgcgccatcc cgagaccgtg aaggcattca cggtcatcaa aagccaaccg
     3601 gtctcatccg ggttcggcga cagtacgttc catggcctga atgcgtttcg attccgcaat
     3661 cgggccggcg attccacccc ggtccgctgg ctgatgtcgc cgatgcagcc gttcgagccg
     3721 gccggccccg ggccccttca gggcaagaat tctctcttcg acgcgctgat caccgcgatt
     3781 caccgtcagc cactgcgatg gcgcctcatc gtcatcgtcg ggcaaccggg cgaccctacc
     3841 aacgacgcca gcgtcgcctg gccggcggat cgccagcagg tcgacgtggg gaccgtgatg
     3901 ctcgaccgag tggagag
//
LOCUS       DATMCV010000034         2937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_334244, whole genome shotgun sequence.
ACCESSION   DATMCV010000034 DATMCV010000000
VERSION     DATMCV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334244"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VNU03_00890"
     CDS             complement(<1..433)
                     /locus_tag="VNU03_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73029.1"
                     /translation="MIKYWLVVMAAVGVLAPAPAQAQISDGVIKIGVLTDMSSLYADL
                     SGQGSVIAARMAVEDFGAAKKGMKVEIISADHQNKADVGSGIARQWLDSEQVDVIMDT
                     PNSAVALAVNQIVKEKGRAFLVSGAASSDLSGKACSPNTVHW"
     gene            complement(471..1175)
                     /locus_tag="VNU03_00895"
     CDS             complement(471..1175)
                     /locus_tag="VNU03_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73030.1"
                     /translation="MSETLLQVADLQAWYGESHVLHGVDFEVRAGEVVTLLGRNGAGK
                     TTTLKSVMGMVPRRRGSVRFRGEELIGRPSNLIARRGLAFCPEERGIFASLSVEENLL
                     LPPVVQPGGLGLDAIYELFPNLRERGRSQGTKLSGGEQQMLAIGRILRTGARLLLLDE
                     PTEGLAPVIVRQIRATIRRLKADGFTILLVEQNFRFAATVADRHYVMERGRVVDMIDN
                     AELPAQTAKLHEYLGV"
     gene            complement(1168..1923)
                     /locus_tag="VNU03_00900"
     CDS             complement(1168..1923)
                     /locus_tag="VNU03_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73031.1"
                     /translation="MADDVILETRGLTKEFRGFLAVRDVSLRVRRGTIHALIGPNGAG
                     KTTCFNLLTHFLVPTRGRITFNGQDITGRRPADIARLGLVRSFQISAVFPHLTTLENV
                     RIALQRRRGRSFDFWRSERVLGAFRGRALELLGAVGLHGFAETLAGELSYGRKRALEI
                     ATTLALEPEMMLLDEPTAGMAHEDVDRISALIKQVAKNRTVLMVEHNLSVVEHLSDTI
                     TVLARGEILAEGDYAAVSANPDVVQAYLGVGHE"
     gene            complement(1927..2718)
                     /locus_tag="VNU03_00905"
     CDS             complement(1927..2718)
                     /locus_tag="VNU03_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWP73032.1"
                     /translation="MSEPRVTYEARDGVATITLSRPRVLNALDLALSAELAEAADAAA
                     RDEAVWVVVVRGAGRAFCSGMDRSVLSAGGIGEAFYRNWIRALNRLEDMDKLSVAVLH
                     GYSIGGGLQLAVACDLRLATTDAVLGLGATRHGLIPDGSILRLARIVGLGRAKELTLL
                     NDHVTPAAALAMGLVNWVVEPPEVDATLAGIVEKAFHSSRTATGHAKRLLHASFHHDP
                     RELIEDVVRAQNACMTSWEMDEANRAWDERREARFYPPPSGTSRP"
     gene            complement(2715..>2937)
                     /locus_tag="VNU03_00910"
     CDS             complement(2715..>2937)
                     /locus_tag="VNU03_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73033.1"
                     /translation="AKVAASMAKVFASEVAMKAATKALQIHGGAGYITEFPIERIFRD
                     AKLTEIGEGTSEIQRMVIAREILGARSQP"
BASE COUNT          421 a         1061 c         1014 g          441 t
ORIGIN      
        1 tccagtggac ggtgtttggc gagcacgcct tgccgctcag gtcggaggag gcggcgcccg
       61 agacgaggaa cgcccggccc ttctccttga cgatctggtt gaccgcgagc gcgaccgccg
      121 agttcggcgt gtccatgatc acgtcgacct gctcggagtc gagccactgg cgtgcaatgc
      181 ccgagcccac gtccgccttg ttctgatggt cggccgagat gatctcgacc ttcatcccct
      241 tcttggccgc tccgaagtcc tccaccgcca tccgggccgc gatcaccgag ccctggccgg
      301 agaggtcggc gtagaggctc gacatgtcgg tgagcacccc gatcttgatg accccgtcgg
      361 agatctgggc ctgggccggc gcgggagcga gcacccccac cgcggccatg acgacgagcc
      421 agtacttgat catgcgttga ttcctttccg tgcggttgaa cgacgcgtga ttataccccg
      481 aggtactcgt gcagcttcgc ggtctgcgcg ggcagctccg cgttgtcgat catgtccacc
      541 acccgccccc gctccatcac gtagtggcgg tcggccaccg tggccgcgaa gcggaagttc
      601 tgctccacga gcaggatcgt gaagccgtcg gccttgagcc gccggatcgt ggcccggatc
      661 tggcggacga tcaccggggc cagcccctcg gtgggctcgt ccagcagcag gagccgggcg
      721 ccggtccgga ggatgcggcc gatggccaac atctgctgct cgcccccgga gagcttggtg
      781 ccctggctcc gcccccgctc ccgcaggttc gggaagagct cgtagatggc gtcgagcccg
      841 agcccgcccg gctgcaccac cgggggcagg agcaggttct cctccacgct caagctcgcg
      901 aagatcccgc gctcctccgg gcagaaggcg agcccccggc gcgcgatcag gttggagggc
      961 cggccgatca gctcctcacc gcggaagcgg accgagccgc gccgccgcgg caccatgccc
     1021 atcaccgact tgagcgtggt ggtcttgccg gccccgttgc ggcccagcag ggtcacgacc
     1081 tcgcccgcgc gcacctcgaa gtccaccccg tggagcacgt gcgactcccc gtaccaggcc
     1141 tggagatcgg ccacctgcag gagggtctca ctcatgcccg acccccaggt acgcctgcac
     1201 gacgtccggg ttggccgaca ccgccgcgta gtcgccctcg gccaggatct ccccccgggc
     1261 cagcaccgtg atggtgtcgg agagatgctc caccacgctc aggttgtgct ccaccatcag
     1321 tacggtgcga ttcttcgcca cctgcttgat gagggccgag atgcggtcca cgtcctcgtg
     1381 ggccatgccg gcggtcggct cgtcgagcag catcatctcg ggctcgaggg ccagcgtggt
     1441 ggcgatctcg agcgcccgct tccgcccgta cgacagctcg cccgcgaggg tctccgcgaa
     1501 gccgtggagg cccaccgcgc ccagcagctc gagggcccgc ccgcggaagg cgcccagcac
     1561 gcgctccgag cgccagaagt cgaaggagcg gccgcgccgg cgctggagcg cgatgcgcac
     1621 gttctcgagc gtggtgaggt gcgggaacac cgccgagatc tggaacgacc gcacgaggcc
     1681 gagccgggcg atgtcggcgg gccgccgccc ggtgatgtcc tgcccgttga acgtgatccg
     1741 gccccgggtg gggacgagaa agtgggtcag caggttgaag caggtggtct tgcccgcccc
     1801 gttggggccg atgagggcgt ggatcgtccc gcgccgcacc cgcaggctca cgtcccgcac
     1861 tgcgaggaag ccccggaact ccttggtgag gccgcgggtc tccaggatga cgtcgtcggc
     1921 catcagctag ggccgcgacg tccccgacgg cggcgggtag aaccgagcct cgcgccgctc
     1981 gtcccaggcc cggttggcct cgtccatctc ccaggaggtc atgcatgcgt tctgggcccg
     2041 caccacgtcc tcgatcagct cgcgcggatc gtggtggaag gaggcgtgca gcagccgctt
     2101 ggcgtgcccg gtcgcggtgc gcgaggagtg gaaggccttc tccacgattc ccgcgagggt
     2161 cgcgtcgacc tccgggggct cgaccaccca gttcaccagg cccatcgcga gggccgccgc
     2221 gggggtgacg tggtcgttga gcagggtcag ctccttggcg cgcccgagtc ccacgatgcg
     2281 ggcgagcctc aggatcgacc cgtccgggat gaggccgtgg cgggtcgccc cgaggcccag
     2341 caccgcgtcg gtggtggcga ggcgcaggtc gcaggccacc gcgagctgca ggccgccccc
     2401 gatcgagtag ccgtggagca ccgcgaccga gagcttgtcc atgtcctcca ggcgattgag
     2461 ggcccggatc cagttgcggt agaacgcctc gccgatcccg cccgcggaca gcacggagcg
     2521 atccatgccc gagcagaacg cgcgccccgc tccgcgcacc accaccaccc agaccgcctc
     2581 gtcccgggcc gcggcgtcgg ccgcctccgc cagctcggcg gagagcgcga ggtcgagcgc
     2641 gttgagcacg cggggtcgcg agagcgtgat ggtggcgacc ccgtcgcgcg cctcgtacgt
     2701 caccctcggc tcgctcacgg ctggctccgc gctcccagga tctcgcgggc gatgaccatg
     2761 cgctggatct cggacgtccc ctccccgatc tcggtcagct tggcgtcgcg gaagatccgc
     2821 tcgatgggga actcggtgat gtagccggca cccccgtgga tctgcagcgc cttggtggcc
     2881 gccttcatcg cgacctcgga ggcgaacacc ttcgccatgg aggccgccac cttggcg
//
LOCUS       DATMCV010000035         5600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_866756, whole genome shotgun sequence.
ACCESSION   DATMCV010000035 DATMCV010000000
VERSION     DATMCV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866756"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VNU03_00915"
     CDS             <1..985
                     /locus_tag="VNU03_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73034.1"
                     /translation="HTRKVRVVLARNPDYFDKGRPYVDEYVILSTPDAATRMAAFRTG
                     QSDVMWLASPSEVDTVKRTNANALVHSYQNTLTPFGVALAQDKPPFNDVRVRRAVSMA
                     IDRQKQVDTVFEGHGILGWGVPYIYYQDKPLTAASFGPWWQYRPAEAKKLLADAGHPN
                     GFETTLFYYEYFPQMSSQVQLVQQDLKRNLNINVKITKLDYTTYYGRYVESKWDGMAW
                     GFQSGHAIGLDERTYQYMHSKSTKNFFRVADPVIDELTTKLRQTPSQADQRALTKKIV
                     DREFDQVLRMWMPYDYGFMVFQPHVRNGAAGALRRTDGYGSSTIARVWLDK"
     gene            1010..1960
                     /locus_tag="VNU03_00920"
     CDS             1010..1960
                     /locus_tag="VNU03_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73035.1"
                     /translation="MYKYAVRRLLLAVPVLLLSSLIVFGLMRVMPGDALTALMGESGN
                     IGERELAKIRKDLRLDLPYHEQYGLWVWQMVSFHPGDSIFTGEPIAVSLAKAIPVTLE
                     LAALAMILGLVIAVPIGVLSATRQDSAADYVGRVVAVSGLSFPDFWLGTLVITFAAIW
                     FHWIPAIGYVSFWESPLRNLQQFLLPAAVLGFRLSAATMRMTRSTVLEVLREDYVRTA
                     WAKGLEGRIVVYKHALKNALIPVVTIVGGQLGVLLAGTVVVETIFALPGMGRLTVEAI
                     LFRDYPVVQTNVMLVAGTLVSLNLLVDLTYAWLDPRIRYQ"
     gene            1977..2876
                     /locus_tag="VNU03_00925"
     CDS             1977..2876
                     /locus_tag="VNU03_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73036.1"
                     /translation="MATVTARAPVHVEAESDVEIGRLRSLWTTIRRKPLGAASATLIM
                     VLVLTAILADVVSPYDPLAAQPEIRLAAPSWEHPFGTDDIGRDVLSRVIHGCRISLWV
                     GLLAVGIGTVAGMIIGLLSGYCEGRTDLILQRVMDGIQAIPGLVLALAIVSVLRPSTT
                     NAMIAIAMVIIPGNSRIVRGAVLSAKQNRYVEAAQAIGCRHPRIILSHILPNVTAPIL
                     VIASIWLGNAILIEATLSFLGVGTQPPTPSWGLMLSSTGRAFMEQAPWLALFPGLAIS
                     LAVLAFNLFGDTLRDAWDPKLRG"
     gene            2881..4422
                     /locus_tag="VNU03_00930"
     CDS             2881..4422
                     /locus_tag="VNU03_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73037.1"
                     /translation="MTARSRRSLAALLCSLVLAASAPGGAAAQARPEGEMRWALYVTL
                     SPAWFDPGEVVGVLTPFWVLTAMHDALVKPMPGGMLTPSLAESWKLSPDNRVYEFKLR
                     EGLKFHNGDPFTAEDVKFSFLRAKGARILQEKVKDIEIAGPYRVRFHLHEPWPDFMTF
                     YGTFATGAGWIVPKKYMEQVGPDGFKKNPVGLGPYKFVSHTPGVELVMEANESYWRKV
                     PSVKRLVYKSVVEATTRMAMLKRGEVDLAYLLDAPQALELKRDPNFKLAFSGGIGTFY
                     LDFLDQADPKSPWHDRRVRLAASHALDRKALSEAETLGASRPTGSMIPPKFEFALPLE
                     PHPYNPARAKQLLAEAGYPNGFDAGDLYPWPPYTTMGEAVGGYLGAIGIKVRLRTMER
                     AAFYSALASKKIHGLCVCINAVYGNAASRLAETVPSDGAFSYAGEADIDALYKQQSRE
                     TDPRKREALLHQIQRTLHERVRFAPIMDYIWPSGVSARVAEPALMLIDPYPWSAPLED
                     VRLKK"
     gene            complement(4412..4597)
                     /locus_tag="VNU03_00935"
     CDS             complement(4412..4597)
                     /locus_tag="VNU03_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73038.1"
                     /translation="MAAEAVTLVVIPAKRLDHLVRTNPTLAVAIIRDLSAKLLATNRL
                     LAAEGERRAAQPGHVTS"
     gene            complement(4567..4788)
                     /locus_tag="VNU03_00940"
     CDS             complement(4567..4788)
                     /locus_tag="VNU03_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWP73039.1"
                     /translation="MSVQTRTFRAGEVIFREGDVPRDEAFLVHAGRVEVRKKVGDEER
                     LLRTFVKGERLGAHPARPPRWPPRRSPSW"
     gene            4859..>5600
                     /locus_tag="VNU03_00945"
     CDS             4859..>5600
                     /locus_tag="VNU03_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP73040.1"
                     /translation="MRLAGKVALVTGAQQGIGRAIALAMAGEGADVGLNFLDDPGAAE
                     RVAGEIRGRGRKVLTVKGDVAQARDVQALVDAVTGGLGPPDILVNNAGVFPRSPFLEL
                     TEREWDHVLGVNLKGGFLCAQAVARAMVAAGRPGAIVNISSSAIRGDARGVHYSASKA
                     GVLGMTRAMALALAPHRIRVNAIAPGLTDTAQPRYGNTEEQIAERAREIPLGRIAQPE
                     EIARIAVFLASEDSGWITGELIHVNGGAY"
BASE COUNT          849 a         1982 c         1924 g          845 t
ORIGIN      
        1 gcacacccgg aaggtgcggg tggtgctggc gcgcaatccc gactacttcg acaagggccg
       61 cccctacgtg gacgagtacg tgatcctgtc gaccccggac gcggcgaccc gcatggccgc
      121 cttccgcacc gggcagagcg acgtcatgtg gctcgcgagc ccgtccgagg tggacacggt
      181 caagcggacg aacgccaacg cgctggtgca ctcctaccag aacaccctga ccccgttcgg
      241 ggtggcgctg gcccaggaca agccgccgtt caacgacgtg cgggtgcgcc gcgcggtctc
      301 catggcgatc gaccgccaga agcaggtgga caccgtcttc gagggccacg gcatcctcgg
      361 ctggggcgtc ccgtacatct actaccagga caagccgctc accgcggcca gcttcgggcc
      421 gtggtggcag taccgccccg cggaggccaa gaagctcctc gccgacgccg gccatccgaa
      481 cggcttcgag acgaccctgt tctactacga gtacttcccg cagatgagct cgcaggtgca
      541 gctcgtgcag caggatctga aaaggaacct caacatcaac gtcaagatca ccaagctcga
      601 ctacacgacc tattacggcc gctacgtcga gagcaagtgg gacggcatgg cgtggggctt
      661 ccagtcgggg cacgcgatcg ggctcgacga gcggacctac cagtacatgc attcgaagtc
      721 gaccaagaac ttcttccgcg tcgccgaccc ggtcatcgac gagctcacca ccaagctgcg
      781 ccagaccccg agtcaggccg atcagcgggc gctcaccaag aagatcgtcg accgcgagtt
      841 cgaccaggtg ctgcggatgt ggatgcccta cgactacggg ttcatggtct tccagccgca
      901 cgtgcgcaac ggggcggcgg gcgcgctgcg gcgcaccgac ggctacggct cctccaccat
      961 cgcccgggtc tggctcgaca agtagggcga gggcctagcc cggcgcgcca tgtacaagta
     1021 cgcggtccgc cgcctcctcc tcgcggttcc ggtcctgctg ctgtcctcgc tcatcgtctt
     1081 cgggctgatg cgggtgatgc ccggcgacgc gctcaccgcc ctcatgggcg agtcgggcaa
     1141 catcggggag cgggagctcg ccaagatccg gaaggacctg cgcctcgacc tgccgtacca
     1201 cgagcagtac gggctgtggg tctggcagat ggtgagcttc cacccgggcg actcgatctt
     1261 caccggggag ccgatcgcgg tgagcctggc caaggcgatc ccggtcaccc tcgagctcgc
     1321 cgcgctcgcc atgatcctgg gcctggtcat cgcggtcccg atcggggtgc tctcggccac
     1381 gcggcaggac agcgcggcgg actacgtggg ccgggtggtc gcggtctccg ggctgtcgtt
     1441 ccccgatttc tggctcggca cgctcgtgat cacgttcgcg gcgatctggt tccactggat
     1501 ccccgccatc ggctacgtga gcttctggga gtcgccgctg cgcaacctcc agcagttcct
     1561 gctgcccgcc gcggtgctcg gcttccgcct ctccgccgcg accatgcgca tgacccgctc
     1621 gacggtgctc gaagtgctgc gcgaggatta cgtgcgcacc gcgtgggcca agggcctcga
     1681 ggggcgcatc gtggtgtaca agcacgcgct caagaacgcg ctgatcccgg tggtgaccat
     1741 cgtgggcggg cagctcggcg tgctcctggc cggcacggtg gtggtggaga cgatcttcgc
     1801 gctgcccggc atgggccggc tcaccgtgga ggcgatcctc ttccgcgact acccggtggt
     1861 gcagaccaac gtcatgctgg tggcgggcac gctggtgtcg ctgaatctcc tggtggacct
     1921 cacctacgcg tggctggatc cgcggatccg ctatcaatga cgaggagtga gcgccgatgg
     1981 cgacggtgac cgcgcgcgcc cccgtccacg tcgaggccga gtccgacgtc gagatcggtc
     2041 ggctccggtc cctctggacc acgatccgcc gcaagccgct cggcgccgcc tccgccaccc
     2101 tcatcatggt gctggtgctg accgcgatcc tggccgacgt ggtctccccc tacgatccgc
     2161 tcgcggccca gcccgagatc cgcctggccg cccccagctg ggagcatccg ttcggcaccg
     2221 acgacatcgg ccgcgacgtg ctcagccggg tcatccacgg ctgccgcatc tcgctctggg
     2281 tggggctgct cgcggtgggc atcgggacgg tggcgggcat gatcatcggc ctgctgtccg
     2341 gctactgcga gggccggacc gacctgatcc tgcagcgggt gatggacggc atccaggcca
     2401 tcccgggcct cgtgctggcc ctggccatcg tgtcggtgct gcggccgagc accacaaacg
     2461 cgatgatcgc catcgcgatg gtcatcatcc cgggcaactc ccgcatcgtg cgcggggcgg
     2521 tgctctcggc caagcagaac cgctacgtgg aggccgcgca ggccatcggc tgccgccacc
     2581 cgcgcatcat cctgagccac atcctgccca acgtgaccgc cccgatcctg gtcatcgcct
     2641 cgatctggct cggcaacgcg atcctgatcg aggccacgct gtccttcctc ggggtcggca
     2701 cccagccacc cacgccctcc tggggcctga tgctcagcag caccgggcgc gcgttcatgg
     2761 agcaggcgcc gtggctggcc ctgttccccg ggctcgccat cagcctcgcc gtgctcgcat
     2821 tcaacctctt cggcgacacg ctgcgggacg cgtgggatcc gaagctacgg ggttagcccg
     2881 atgaccgctc gctctcgccg ctcgctcgcc gcgctgctgt gctcgctggt cctggccgcc
     2941 tccgcgccgg gcggcgccgc ggcccaggcc cgcccggaag gggagatgcg ctgggcgctc
     3001 tacgtgacgc tctcgccggc ctggttcgat cccggcgagg tggtgggcgt tctcaccccg
     3061 ttctgggtgc tcaccgccat gcacgacgcg ctggtgaagc cgatgccggg cggcatgctc
     3121 accccgagcc tcgccgagtc gtggaagctg agcccggaca accgggtgta cgagttcaag
     3181 ctgcgcgagg gcctcaagtt ccacaacggc gaccccttca ccgcggagga cgtgaagttc
     3241 agcttcctgc gcgccaaggg cgcgcggatc ctgcaggaga aggtcaagga catcgagatc
     3301 gcgggaccgt accgggtgcg cttccacctg cacgagccct ggcccgactt catgaccttc
     3361 tacggcacgt tcgccaccgg cgcgggctgg atcgtcccca agaagtacat ggagcaggtg
     3421 ggacccgacg gcttcaagaa gaacccggtc gggctcgggc cctacaagtt cgtgagccac
     3481 actccgggcg tggagctcgt gatggaggcc aacgagagct actggcgcaa ggtgccctcg
     3541 gtgaagcggc tggtctacaa gagcgtggtg gaagcgacca cgcggatggc catgctgaag
     3601 cgaggcgagg tcgacctcgc gtacctcctc gacgcgcccc aggccctcga gctgaagcgc
     3661 gatccgaact tcaagctcgc cttctccggc ggcatcggga cgttctacct ggacttcctc
     3721 gaccaggccg atccgaaatc gccgtggcac gaccggcggg tccggctcgc ggccagccac
     3781 gcgctcgacc gcaaggcgct gagcgaggcc gagaccctcg gggcctcccg gcccaccggc
     3841 agcatgatcc cgcccaagtt cgagttcgcg ctgccgctcg agccccaccc ctacaacccg
     3901 gcccgcgcca agcagctcct ggccgaggcg gggtacccga acggattcga cgcgggcgat
     3961 ctctacccgt ggccgccgta caccacgatg ggggaggcgg tcggtggcta cctgggcgcg
     4021 atagggatca aggtgcgcct gcggacgatg gagcgcgccg cgttctactc ggcgctggcc
     4081 tccaagaaga tccacgggct ctgcgtgtgc atcaacgcgg tctacggcaa cgccgcctcg
     4141 cgcctggccg agacggtgcc gagcgacggc gcgttctcgt acgcgggcga ggccgacatc
     4201 gacgcgctct acaagcagca gagccgggag accgaccccc gcaagcggga ggccctgctg
     4261 caccagatcc agcggacgct ccacgagcgg gtccgcttcg cgccgatcat ggactacatc
     4321 tggccgagcg gggtgagcgc gcgggtggcc gagcccgccc tgatgctgat cgatccctac
     4381 ccctggtcgg cgcccctgga ggacgtgcgg ctcaagaagt gacgtgcccc ggttgggccg
     4441 cgcgccgctc gccctccgcg gcgaggaggc ggttggtggc gagcagcttg gccgagaggt
     4501 cgcggatgat ggccaccgcc agggtggggt tcgtgcgcac caggtgatcg agccgcttcg
     4561 ccgggatcac cacgagggtg accgcctcgg cggccaccgc ggtggccgag cggggtgagc
     4621 ccccagacgc tctcccttga cgaaggtgcg caggagccgc tcctcgtccc cgaccttctt
     4681 gcggacctcg acccggccgg cgtggaccag gaaggcctcg tcgcgcggga cgtcgccctc
     4741 gcggaagatc acctcgccgg cgcggaaggt gcgggtctgg acgctcacag gcttgcggtt
     4801 catgggcttc gacccctcta taataagcgg gcggcgaacc gaataccgga gggaaccgat
     4861 gcgactcgcg ggcaaggtgg cgctggtcac gggcgcgcag caggggatcg gacgagccat
     4921 cgcgctggcg atggcggggg agggcgccga cgtcggcctg aacttcctcg acgacccggg
     4981 ggcggccgag cgcgtggccg gcgagatccg gggtcgcggg cgcaaggtcc tcaccgtcaa
     5041 gggcgacgtg gcgcaagcgc gcgacgtgca ggccctggtc gacgcggtca ccggcgggct
     5101 cgggccgccc gacatcctgg tcaacaacgc gggcgtcttc ccgcgctcgc cgtttctcga
     5161 gctgaccgag cgcgagtggg accacgtgct cggggtgaat ctcaagggcg gcttcctctg
     5221 cgcccaggcg gtggcgcgcg cgatggtggc ggcgggccgg ccgggcgcca tcgtcaacat
     5281 ctcgtcctcc gcgatccgcg gcgacgcccg gggcgtgcac tactcggcga gcaaggccgg
     5341 cgtgctcggc atgacccgcg cgatggcgct ggctctggcc ccccaccgca tccgggtcaa
     5401 cgcgatcgcg ccgggcctca ccgacaccgc gcagccccgc tatggcaaca ccgaggagca
     5461 gatcgcggag cgcgcgcgcg agatcccgct cgggcgcatc gcccagccgg aggagatcgc
     5521 gcggatcgcg gtgttcctcg cctcggagga ttcgggctgg atcaccggcg agctgatcca
     5581 cgtcaacggc ggggcctaca
//
LOCUS       DATMCV010000036         7508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_201457, whole genome shotgun sequence.
ACCESSION   DATMCV010000036 DATMCV010000000
VERSION     DATMCV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201457"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..860)
                     /locus_tag="VNU03_00950"
     CDS             complement(66..860)
                     /locus_tag="VNU03_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP73041.1"
                     /translation="MSDATKYEAIRYEQADGVATVTLNRPDVHNAMNQAMRRELLEVF
                     TRLRGDDTVRAVVVTGAGDKAFSAGADIREFLEPPVPTQFREARRRVDFRREMERCPQ
                     PIIAAIRGYAFGGGLEMALACDIRIAAEDAQLGLTEINLAIIPGGGGTQRLPRLVGRG
                     KALEMIMTGMRIPAAEALRIGLVERVVPVAELPSAAQALARTLADKAPVALRYAKEAV
                     VGGLGLPLEDGIRLENDLATLLRTTEDRVEGAKAFVEKRKPKWTGR"
     gene            complement(857..1996)
                     /locus_tag="VNU03_00955"
     CDS             complement(857..1996)
                     /locus_tag="VNU03_00955"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP73042.1"
                     /translation="MKVVDLTSYIAGSYGAMMLADLGADVIKVEAIEGDSFRELPGFY
                     GWNRGKRSIAVNMKDPDGRGVVHRLARDADVVMENMRPGVVERLGVDYETLRAINPRI
                     IYSSVTAFGSDGPYKERPGFDPLLQAMGGVMTLQGLGAAPQYLRIAVTDYYCAALACQ
                     MVLAALFARERTGKGQRVQTSLLQAVLALQSGNVLAYPGLEPPTFRTTPTYRLYQGGD
                     GGWFFLAVGNQSFWMKLCKAIGHEELARDPRFGSWFARRDHADVLMPLLESAFASKPC
                     EEWLRILAEHDIPAAPTQSLQEFMQDPAVLHHKMVVQYDHPELGPLSLMGQPLRFSET
                     QAPDAGPPPMLGQHTAEVLRQAGYADPEIADLRRRGVVAGKDSAP"
     gene            complement(2089..3216)
                     /locus_tag="VNU03_00960"
     CDS             complement(2089..3216)
                     /locus_tag="VNU03_00960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73043.1"
                     /translation="MNFSFSEDQVLLRSSVRAALDEQCTPARVRAMMDDDVSGYDEAL
                     WGEMAKLGWLGLPFPEEQGGAGLGLVELALVLEEMGRAAYPGPYFASVVLGGLGLMLG
                     GSPAQKEKWLPAIASGQARASAALLEDGLDWDPAATAATATRAGDGWRLTGIKRFVPW
                     AHVADVVLVPARAPEGLCLFLVDPRGAGVTLTPMVGIDLGNRWSELRLTDAPVPADAL
                     VGPAGSAGPVLDGLLRRAAVGASAEMLGAARRCLDMSVGYAKVREQFGQLIGSFQAIR
                     HRCAEMLVEVENSHSAVYYAAWALAAGAEDAAGAASICKSYVSESARKVCGDAIQVHG
                     GIGFTWEYDLHLYFKRAKSLEPLYGDAAHHRELIVRGITGA"
     gene            complement(3213..4427)
                     /locus_tag="VNU03_00965"
     CDS             complement(3213..4427)
                     /locus_tag="VNU03_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73044.1"
                     /translation="MDLTYTPEDVAFRKQVRAWLEHHMPRHIKNLDERRAWHRRLYEA
                     GYLGMGWPREYGGQGARPMEQAIVADEMARAGAPAPTNGLGLGIAGPTIVVHGTDAQK
                     RRYLRKILTAEEMWCQLYSEPNAGSDLAALRTAAVDRGDHFEVNGQKIWTSGGANADW
                     GLLLARTDPAVPKHKGITCFLMDMRQPGVDVRPLKQITGSSEFCEVFMTDARVEKENM
                     VGRLGEGWGIAQTTLGYERGGRALARVTTYASQYRQLVDAARRLKRNGRPIVDDPVTR
                     QKLGRVWADLEVERYGALRTLTMLERGEHPGAGGSITKLSYSEFEKRFMELAEEILGP
                     YGQLTDGVPPEYRLEVDTAIGDHGTWAYAFLWSRAGTIYAGSSEIQKNVIGERILGLP
                     KEARADRVGGTR"
     gene            4583..5773
                     /locus_tag="VNU03_00970"
     CDS             4583..5773
                     /locus_tag="VNU03_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73045.1"
                     /translation="MTIDRRAWLQGAGAVTILAAGGVAWRAHEQGVLAGGSGPAYEPW
                     ATWRRDAAEGPLALVRAGILAANPHNTQPWLFQVGAARVALLADTARNLGSFDPYLRE
                     LHIGLGCAIENMARAARAYGYEATVTLRGGTLAGPASPPSPEPVAVLELAPARPRPDA
                     LFDAIGRRHTDRAAYDAARPVPSDALEAMWEAFKEDPEVRLFLYSATEERRQFGAAVV
                     AATERIVGDPRMIEDSQRWFRHRWADVQRLRDGPTLDAAGLPAWMTVAAKLLPPPSPE
                     TNHRYWLDATRDVQVKTTALFGLIAVRDLYDRAQALRAGRLWQRLHLLAVSRGLAMQP
                     INQPVELVDRERALGREPRAAATLAALTGAAEWKPTFAFRMGFPTRAAAPSPRRPVDA
                     VVVP"
     gene            complement(5770..6204)
                     /locus_tag="VNU03_00975"
     CDS             complement(5770..6204)
                     /locus_tag="VNU03_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWP73046.1"
                     /translation="MRVAERMRREPVAVGPGESLGLAGRLLARHRIDALPVVRRSALV
                     GLLAAVDVDRAHPSVASTLAVREIPARLDRVRIDRVIQPSVTSVGRRTPLVDAIRLMR
                     ARRLPALPVTEGERLVGLLSEDDLLALLATFLEEPTGPRAAG"
     gene            complement(6191..7057)
                     /locus_tag="VNU03_00980"
     CDS             complement(6191..7057)
                     /locus_tag="VNU03_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWP73047.1"
                     /translation="MQIKTLLTRPAITTEPATPLRDARALMRRRGIRHLPVIERHRLV
                     GMLAERDIERADVSSIPEIARHDWAVAVAGLVVADVMARDPLALHPETPVAEAARLAR
                     DRRVDAFAVVDDGAVTGVVSRSDLLAVLSGLLEHRHPTGLGHVLAATSLRPGCRRALG
                     EALRIAATTGATVTALHVLPAARPIAGLEAEAMVAIERARRHVADEAMAILREAGRDR
                     DVQYEVAEGPVAREIAQRAEELAADLIVIGTAARRGVLGGLVTTLGDRLARLAPCPVL
                     AIPRSGGRDARR"
     gene            complement(7071..>7508)
                     /locus_tag="VNU03_00985"
     CDS             complement(7071..>7508)
                     /locus_tag="VNU03_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWP73048.1"
                     /translation="RMRSSAYRRLLVLLDGTERGERALTWARHLVRGPGSAVHLLMIE
                     PAGRVVGVGGRTVAFVDQLEDAARAAARAYLASVAGRLREDGMTVWTHVRFGAPVPVT
                     RAVIQELDADVLVVTDGVIRRPDDLGAVPIPVLTSGPRCLRSA"
BASE COUNT          982 a         2724 c         2807 g          995 t
ORIGIN      
        1 ggggatggcg gcggcggtga atcggtctcg ggtgctagca ggtcactccg agcgccgtcg
       61 ccgcgtcagc ggccggtcca cttgggcttg cgcttctcga cgaaggcctt ggcgccctcg
      121 acgcggtcct cggtggtgcg cagcagggtg gcgaggtcgt tctcgagacg gatgccgtct
      181 tcgagcggca ggccgaggcc gcccaccacc gcttctttcg cgtagcgcag cgccaccggc
      241 gccttgtccg ccagcgtgcg ggcgagggcc tgcgcggccg acggcagctc ggccaccggc
      301 accacgcgct cgaccaggcc gatgcgcagg gcctccgcgg ccgggatgcg catgccggtc
      361 atgatcatct cgagcgcctt gccgcggccg accagacgcg ggaggcgctg ggtaccgccg
      421 cctcccggga tgatggccag gttgatctcg gtgagcccga gctgcgcgtc ctcggccgcg
      481 atgcggatgt cgcaggcgag cgccatctcg aggccgcccc cgaaggcgta gccgcggatc
      541 gccgcgatga tcggctgcgg gcagcgctcc atctcgcgcc ggaagtccac gcgccgccga
      601 gcctcgcgga actgggtcgg caccgggggc tcgaggaact cgcggatgtc ggcgcccgcg
      661 gagaaggcct tgtcgcccgc cccggtcacc accaccgcgc gcacggtgtc gtcgccgcgc
      721 aggcgcgtga acacctccag cagctcgcgg cgcatcgcct ggttcatcgc gttgtgcacg
      781 tcggggcggt tcagggtgac ggtggctacc ccgtcggcct gctcgtagcg gatggcctcg
      841 tacttggtcg cgtcgctcat ggggcggagt ccttgccggc caccacgccg cggcggcgca
      901 ggtcggcgat ctcgggatcg gcatagcccg cctggcgcag cacctcggcg gtgtgctggc
      961 cgagcatggg cggcgggccc gcgtcggggg cctgcgtctc ggagaagcgg agcggctggc
     1021 ccatcaggga gagcgggccc agctccgggt gatcgtactg gacgaccatc ttgtggtgca
     1081 gcaccgcggg gtcctgcatg aactcctgga gcgactgggt gggggcggcc gggatgtcgt
     1141 gctcggccag gatgcgcagc cactcctcgc acggcttgga ggcgaacgcg ctctcgagca
     1201 ggggcatcag cacgtcggcg tggtcgcggc gcgcgaacca ggagccgaag cgcgggtcgc
     1261 gggccagctc ctcgtggccg atcgccttgc agagcttcat ccagaacgac tggttgccga
     1321 cggcgaggaa gaaccagccg ccgtcgccgc cctggtagag ccgataggtc ggcgtggtgc
     1381 ggaaggtggg gggctcgagc cccggatagg ccagcacgtt gccggactgc agcgcgagca
     1441 ccgcctggag cagcgaggtc tgcacgcgct gtcccttgcc ggtgcgctcg cgcgcgaaga
     1501 gggcggccag caccatctgg caggccagcg cggcgcagta gtagtcggtg accgcgatgc
     1561 gcaggtactg gggcgcggcg ccgaggccct ggagggtcat gacgccgccc atcgcctgga
     1621 gcaggggatc gaagccgggg cgctccttgt aggggccgtc cgagccgaag gcggtgacgc
     1681 tcgagtagat gatgcgcggg ttgatggcgc ggagggtctc gtagtccacg cccaggcgct
     1741 cgacgacgcc ggggcgcatg ttctccatca ccacgtccgc gtcgcgggcg aggcgatgga
     1801 cgacgccgcg gccgtcgggg tccttcatgt tgaccgcgat cgagcgcttg ccgcggttcc
     1861 agccgtagaa gccgggcagc tcgcggaagg agtcgccctc gatcgcctcg accttgatca
     1921 cgtcggcgcc caggtcggcc agcatcatcg ccccgtacga gccggcgatg tacgaggtca
     1981 ggtcgacgac cttcacgcct gccagggctc ccccctcact tgccaaggct cccccctcac
     2041 cctgccctct cccccctggg gggtaagggg gccggtcttc gccggtcgtc atgcgccggt
     2101 gataccccgg acgatgagct cgcggtggtg ggccgcgtcg ccgtagaggg gctcgagcga
     2161 cttggcgcgc ttgaagtaga ggtggaggtc gtactcccag gtgaagccga tgccgccgtg
     2221 gacctggatc gcgtcgccgc agaccttgcg ggccgactcg ctgacgtagg acttgcagat
     2281 ggaggcggcg ccggccgcgt cctccgcccc ggcggcgagc gcccacgccg cgtagtagac
     2341 cgcggagtgc gagttctcga cctcgaccag catctccgcg cagcggtggc ggatggcctg
     2401 gaacgagccg atgagctggc cgaactgctc acgcaccttg gcatagccca cgctcatgtc
     2461 gaggcagcgg cgggccgcgc ccagcatctc ggcggaggcg cccaccgcgg cgcggcgcag
     2521 gagcccatcc aacaccgggc ccgcgctgcc ggccgggccc accagggcgt cggcgggcac
     2581 cggcgcgtcg gtcaggcgca gctccgacca gcggttgccg aggtcgatgc cgaccatggg
     2641 cgtgagcgtc accccggccc cgcgcgggtc caccaggaag aggcagaggc cctcgggcgc
     2701 gcgggccggg accagcacca cgtcggccac gtgggcccac ggcacgaagc gcttgatgcc
     2761 ggtcaggcgc cagccgtcgc cggcgcgcgt ggcggtcgcg gcggtggcgg cgggatccca
     2821 gtcgagcccg tcctccagca gcgccgcgct cgcgcgggcc tggcccgagg cgatcgcggg
     2881 cagccacttc tccttctggg ccgggctgcc gcccagcatc agcccgagcc cgcccagcac
     2941 cacgctcgcg aagtaggggc cgggatacgc ggcgcggccc atctcttcga gcaccagcgc
     3001 cagctcgacc aggccgaggc cggcgccacc ctgctcctcg gggaagggca ggccgagcca
     3061 gccgagcttg gccatctcgc cccagagcgc ctcgtcgtag ccgctgacgt cgtcgtccat
     3121 catggcgcgc acgcgggcgg gcgtgcactg ctcgtccagc gccgcgcgga ccgagctgcg
     3181 cagcaggacc tgatcttcgc tgaacgagaa attcatcgag tacctccgac acggtcggcc
     3241 cgggcttcct tcggcagccc caggatgcgc tccccgatga cgttcttctg gatctcggag
     3301 gagccggcgt agatggtgcc cgcgcgcgac cacaggaacg cgtaggccca ggtgccgtga
     3361 tcgccgatcg cggtgtcgac ttccaggcga tactccggcg gcaccccgtc ggtgagctgg
     3421 ccgtaggggc ccaggatctc ctcggccagc tccatgaagc gcttctcgaa ctcggagtac
     3481 gagagcttcg tgatggagcc gcccgccccg ggatgctcgc cccgctccag catggtcagc
     3541 gtgcggagcg cgccgtagcg ctcgacctcg aggtcggccc agacgcgccc gagcttctgc
     3601 cgcgtcaccg ggtcgtccac gatgggccgc ccattgcgct tcagccggcg cgcggcgtcg
     3661 acgagctggc ggtactgcga ggcgtaggtg gtgacgcggg ccagcgcgcg gccgccgcgc
     3721 tcgtagccga gcgtggtctg ggcgatgccc cagccctcgc cgagccgtcc gaccatgttc
     3781 tccttctcga cccgcgcgtc ggtcatgaag acctcgcaga actccgacga gccggtgatc
     3841 tgcttcagcg ggcgcacgtc caccccgggc tggcgcatgt ccatcaggaa gcacgtgatg
     3901 cccttgtgct tggggacggc gggatcggtg cgcgcgagga ggaggcccca gtccgcgttg
     3961 gccccgccgc tggtccagat cttctgaccg ttgacctcga agtggtcgcc gcggtccacc
     4021 gcggcggtgc ggagcgcggc caggtcggag cccgcgttgg gctcggagta gagctggcac
     4081 cacatctcct ccgcggtgag gatcttgcgg aggtagcgcc gcttctgcgc gtcggtgccg
     4141 tgcaccacga tggtggggcc cgcgatgccc aggcccagcc cgttcgtggg ggcgggcgcg
     4201 cccgcgcgcg ccatctcgtc ggccacgatc gcctgctcca tcggccgcgc gccctggccg
     4261 ccgtactccc tgggccagcc catgccgaga tagccggcct cgtagaggcg gcgatgccag
     4321 gcccgtcgct cgtcgaggtt cttgatgtgg cgcggcatgt ggtgctcgag ccacgcgcgc
     4381 acctgcttgc ggaaggcgac gtcctcgggg gtgtaggtca gatccattgg cgggggcctc
     4441 ctcacgtcgg ccaggccggt tgggtcagta gcggattctt gtcagccgga gtctctaaga
     4501 ggtagccccg gttctagcac gggccgcgag ggacggtcaa ccgccgacgg cgcgccgtgc
     4561 taccgtcgcg ggcgagcggc cgatgacgat cgatcggaga gcgtggctcc agggggcggg
     4621 cgccgtcacc atcctggccg cggggggagt ggcctggcgc gcgcacgagc aaggcgtgct
     4681 cgcgggtggg agcgggccgg cctacgagcc ctgggccacc tggcgtcgcg acgcggccga
     4741 ggggccgctg gcgctggtgc gggccggcat cctcgccgcc aacccccaca acacgcagcc
     4801 gtggctcttc caggtgggcg ccgcgcgggt ggcgctcctg gccgataccg cccggaacct
     4861 gggctccttc gacccctacc tgcgcgagct gcacatcggc ctcggctgcg cgatcgagaa
     4921 catggcgcgc gccgcccgcg cctacggcta cgaggcgacg gtgacgctcc gcggggggac
     4981 gctcgcgggc ccggcctcgc cgccgagccc ggagccggtc gcggtcctcg agctggcgcc
     5041 ggcccgcccg cgcccggatg cgctcttcga cgccatcggg cgccggcaca ccgatcgcgc
     5101 cgcctacgac gcggcgaggc cggtgccctc cgacgcgctc gaggcgatgt gggaggcctt
     5161 caaggaggac cccgaggtgc gcctgttcct ctactcggcg acggaggagc ggcgccagtt
     5221 cggcgcggcg gtggtcgcgg ccaccgagcg catcgtgggc gacccgcgga tgatcgagga
     5281 cagccagcgc tggttccgcc accgctgggc cgacgtgcag cggctgcgcg acggccccac
     5341 cctcgacgcg gcggggctgc ccgcctggat gaccgtggcg gccaagctgc tgcccccgcc
     5401 atcacccgag accaaccacc gctactggct cgacgccacc cgcgacgtgc aggtgaagac
     5461 gaccgcgctc ttcggcctca tcgcggtgcg ggatctctac gaccgcgcgc aggcgctgcg
     5521 ggcggggcgg ctctggcagc gcctgcacct gctggcggtg agccgcggcc tcgcgatgca
     5581 gccgatcaac cagccggtcg agctggtgga tcgcgagcgc gcgctcggcc gggagccgcg
     5641 cgcggccgcg acgctcgcgg ccctcaccgg cgccgccgag tggaagccga ccttcgcgtt
     5701 ccggatgggc tttcccacgc gggccgccgc gccgagcccg cggcgtccgg tggacgcggt
     5761 ggtcgtgccc tagcccgcgg cgcggggccc cgtcggctcc tcgagaaacg tggccaggag
     5821 cgccagcagg tcgtcctcgg agagcagccc caccaggcgc tcgccctcgg tcaccgggag
     5881 cgcggggagc cggcgcgccc gcatcaggcg aatcgcgtcg acgagcggag tgcgccgtcc
     5941 gaccgaggtg acggacggct ggatgacgcg atcgatccgg acgcggtcga gacgggcggg
     6001 gatctcgcgg accgcgagcg tgctggccac ggacggatgc gcgcgatcga cgtccaccgc
     6061 ggcgagcagg ccgacgagcg cgctccggcg gaccaccggg agcgcgtcga tccggtggcg
     6121 ggccaggagg cggccggcga ggccgaggct ctcgcccggg ccgaccgcga ccggctcccg
     6181 ccgcatccgc tcagcgacgc gcatcgcgcc ctccgctgcg gggaatggcc agcaccgggc
     6241 acggagccag ccgcgcgagc cgatcgccca gcgttgtgac gagcccgccc aggacgccgc
     6301 ggcgcgccgc cgtgccgatg acgatgaggt ccgcggcgag ctcctcggcg cgctgcgcga
     6361 tctcgcgcgc gaccggaccc tccgccacct cgtactgcac gtcccgatcg cggcccgcct
     6421 cgcgcaggat ggccatcgcc tcgtcggcga cgtgacgccg cgcccgctcg atggccacca
     6481 tcgcctcggc ctcgaggcca gcgatcgggc gggcggcggg cagaacgtgc agcgcggtca
     6541 cggtggcgcc ggtggtggcc gcgatgcgca gcgcctcgcc cagcgcgcgg cggcagccgg
     6601 gccgcaggct cgtggcggcg agcacgtggc cgagcccggt cgggtggcga tgctccagca
     6661 ggccgctcag caccgcgagc aggtcgctcc ggctgaccac gcccgtcacc gcgccgtcgt
     6721 cgaccaccgc gaacgcgtcc acccgccggt cgcgggcgag gcgggccgcc tcggccaccg
     6781 gcgtctcggg atggagcgcc agcgggtcgc gcgccatcac gtccgccacc acgagaccgg
     6841 cgaccgcgac cgcccagtcg tggcgggcga tctccgggat gctcgagacg tcggcccgct
     6901 cgatgtcgcg ctccgcgagc atgcccacca ggcggtggcg ctcgatcaca ggcaggtggc
     6961 ggatcccgcg gcggcgcatg agcgcgcgcg catcgcggag gggagtggcc ggctcggtcg
     7021 tgatggccgg tcgggtgagc agggtcttga tctgcatggg ctcctctctt ctatgcggac
     7081 cgcaagcatc gcgggccgct cgtcagcacg ggaatcggga cggcgccaag atcatccggg
     7141 cgtcgaatca cgccgtcggt cacgacgagg acgtcggcgt ccagctcctg gatgacggcc
     7201 cgggtcaccg ggaccggcgc gccgaagcgc acgtgggtcc acactgtcat cccgtcctcg
     7261 cgcaggcggc ctgcgaccga cgcgaggtag gcgcgcgcgg cggcccgcgc ggcgtcctcg
     7321 agctgatcga cgaaggccac cgtccgcccg ccgacaccca ccacacgccc ggccggctcg
     7381 atcatcagga ggtggaccgc gctgccgggt ccccggacca ggtggcgcgc ccaggtgagg
     7441 gcgcgctcgc cgcgctcggt tccgtccagc agaacgagaa ggcgtcggta agcgctcgat
     7501 cgcatgcg
//
LOCUS       DATMCV010000037         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_601068, whole genome shotgun sequence.
ACCESSION   DATMCV010000037 DATMCV010000000
VERSION     DATMCV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601068"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..778
                     /locus_tag="VNU03_00990"
     CDS             101..778
                     /locus_tag="VNU03_00990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWP73049.1"
                     /translation="MPKEVEERPPHRDELFRTTLPLDPGRTALLVVDMQRGFVEEGQV
                     MEVPPAREALPRIQELLAIFREKGLPVVFTEFTYSPAAPLLVGELHPEHRRAAPGAAT
                     GFGRPSSSCLEGDPSASVVAELAPRADELVVRKHYYDGFNGTVLDGALRARGVTTLVV
                     TGTMTDICVLATVIGAFNREYRVLVVEDGVATLWPEIQRATLDIIRRAYARVLSAKEL
                     ADGLARW"
     gene            804..1733
                     /locus_tag="VNU03_00995"
     CDS             804..1733
                     /locus_tag="VNU03_00995"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWP73050.1"
                     /translation="MTHGLRFHGCMPANLLPFTADLAIDEPAYRKHLRWLVDAPGVTG
                     LVANGHAAEVASLTREERRRTLDVALDEVAGKVPVVTGIYSDGTLEAVELARDARAAG
                     ATGLLVFPPTLFMWGAQLKPDMVLRHFQTLADAVDLPLVIFEYPVASGIGYSPETLAE
                     LCKIPTVAAVKDWSNEIVSYEKNLRAVRATGRPVAVMSSFTMSLMASFLLGADGCISG
                     MGSVTADAQAALFAAVQAGRLDEARRLNERMAPLVNVFYAPPFVDMHNRMKEALAILG
                     RIPAAHVRPPLTAISEDERHRIRLALRASGLSS"
     gene            1895..>2817
                     /locus_tag="VNU03_01000"
     CDS             1895..>2817
                     /locus_tag="VNU03_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWP73051.1"
                     /translation="MRVLPGGVLGGYYAPQELAFVVREAHGPRLHDFSGREYIDYILG
                     SGPLVLGHAHPAVVAAVEAQLRKGTTYFQLSEPTLSLAEEICRAVPCAEQVRFCGSGS
                     EATFFALRVARAYRRRDKILKFEGGFHGVHDYSLMSVGPREPKAFPAPTPDSAGIPHA
                     IQGEVLIAPFNDLAEAEAMIEAHHHDLAAVIMEPFQRLIVPQPGFLQGVRAITARHDI
                     PLVFDEIVTGFRFAYGGAQQYYGVVPDLAAFGKIVGGGFPLAAVAGRRDFMRHFSHDL
                     DGTGEFVQQAGTLNGNPIAAAAGLATLAELRR"
BASE COUNT          404 a         1042 c          958 g          413 t
ORIGIN      
        1 gagggcgcgg tcaaggcgat ccggcacatc ccgaacgcgt tctccgtatc cgcctggtag
       61 tgcgaggcgc agccaaaggc gagccgagcg cataccaaaa atgcctaagg aagtagagga
      121 gcgcccgccg caccgcgacg agctgttccg caccaccctg ccgctcgatc ccggccgcac
      181 cgcgctcctc gtcgtcgaca tgcagcgcgg gttcgtggaa gagggccagg tcatggaggt
      241 cccgccggcc cgtgaggcgc tgccgcggat ccaggagctg ctcgcgattt tccgcgagaa
      301 gggcctgccg gtggtcttca ccgagttcac gtactcgccc gccgcgccgc tcctggtcgg
      361 cgagctccac ccggagcacc ggcgcgcggc gccgggcgcc gcgaccggct tcggccggcc
      421 ctcgtcctcc tgcctcgagg gcgatccgag cgcgagcgtc gtcgcggagc tggcgccgcg
      481 cgccgacgag ctcgtggtgc gcaaacacta ctacgacggc ttcaacggga ccgtgctgga
      541 cggagccctc cgggcccgcg gggtcaccac cctggtggtc accgggacca tgaccgacat
      601 ctgcgtgctc gccaccgtga tcggcgcctt caaccgggag taccgcgtcc tggtggtgga
      661 agacggagtg gccacgctct ggcccgagat ccagcgcgcc acgctcgaca tcatccgccg
      721 cgcctacgct cgcgtcctct cggccaagga gctggccgac gggctggcgc gctggtgagg
      781 ggcgccatgc taaggtcgac accatgaccc acggactgcg attccacggc tgcatgccgg
      841 ccaacctcct gccgttcacc gccgacctcg ccatcgacga gcccgcctac cgcaagcatc
      901 tccgctggct cgtggacgcg cccggcgtca ccggcctggt ggccaacggc cacgcggccg
      961 aggtggcctc gctcacccgc gaggagcgcc ggcgcacgct cgacgtggcc ctcgacgagg
     1021 tcgcgggcaa ggtgccggtg gtgaccggca tctactcgga cggcacgctc gaagcggtgg
     1081 agctggcgcg cgacgcacgg gccgcgggcg cgaccgggct gctggtcttc ccgcccacgc
     1141 tcttcatgtg gggcgcgcag ctcaagccgg acatggtgct ccgtcacttc cagaccctcg
     1201 ccgacgcggt cgaccttccg ctcgtgatct tcgagtaccc ggtcgcctcc ggcatcggct
     1261 acagccccga gacgctggcc gagctctgca agatcccgac ggtcgcggcg gtcaaggact
     1321 ggtccaacga gatcgtctcc tacgagaaga acctgcgggc ggtgcgcgcg acggggcgtc
     1381 cggtggcggt catgtcctcg ttcaccatgt cgctgatggc gagcttcctg ctcggcgccg
     1441 acgggtgcat ctccgggatg ggcagcgtga ccgcggacgc gcaggcggcc ctgttcgccg
     1501 cggtgcaggc gggccgcctc gacgaggcac gccggctcaa cgagcgcatg gcgccgctcg
     1561 tcaacgtgtt ctacgccccg ccgttcgtgg acatgcacaa ccggatgaag gaggccctcg
     1621 ccatcctcgg ccggatcccg gccgcgcacg tgcggccgcc cctcaccgcg atctccgagg
     1681 acgagcgcca ccgcatccgg ctcgcgctac gggcctccgg cctgtcctcc tagccgcact
     1741 cgcggtgcgg tcccgggact cgaccgtgta agtgcaccct tgcacggtgg agagctttac
     1801 ggtataaaac ccgccaatct ctccgtccca gcgaggtcga atggcaggtc cggccggtcg
     1861 tagccccgac gaagaagctc tgctccagaa ggcgatgcgg gtcctcccgg gaggtgtcct
     1921 gggtggatac tatgcgccgc aggagctcgc gttcgtggtc cgcgaggcgc acggtccacg
     1981 cctccacgac ttctcgggcc gcgagtacat tgactacatc ctgggaagcg gcccgctcgt
     2041 gctgggccac gcccaccccg cggtggtggc cgcggtggaa gcgcagctcc gcaagggcac
     2101 gacctacttc cagctcagcg agcccaccct ctctctcgcg gaggagatct gccgcgcggt
     2161 gccgtgcgcg gagcaggtgc gcttctgcgg cagcggcagc gaggccacct tcttcgcgct
     2221 gcgggtcgcg cgcgcctacc gacgacggga caagatcctc aagttcgagg gcggcttcca
     2281 cggggtgcac gactactccc tgatgagcgt cggcccgcgc gagccgaagg cgttcccggc
     2341 cccgaccccg gactcggccg gcatcccgca cgcgatccag ggcgaggtcc tgatcgcccc
     2401 gttcaacgac ctggccgagg ccgaggccat gatcgaggcg caccaccacg atctcgccgc
     2461 ggtgatcatg gagccgttcc agcggctgat cgtgccgcag ccgggctttc tccagggcgt
     2521 gcgggcgatc accgcgcggc acgacatccc gctggtcttc gacgagatcg tcaccggctt
     2581 ccgcttcgcc tacggcggcg cccagcagta ctacggggtg gtgccggacc tggccgcctt
     2641 cggcaagatc gtcggcgggg gcttcccgct cgccgcggtg gcgggccggc gcgacttcat
     2701 gcggcacttc tcccacgacc tcgacggcac cggagagttc gtgcagcagg cgggcacgct
     2761 caacggcaac ccgatcgcgg ccgcggccgg cctcgccacc ctggccgagc tgcgccg
//
LOCUS       DATMCV010000038         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_135267, whole genome shotgun sequence.
ACCESSION   DATMCV010000038 DATMCV010000000
VERSION     DATMCV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135267"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VNU03_01005"
     CDS             <1..325
                     /locus_tag="VNU03_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73052.1"
                     /translation="AKEFLTWLMEKEQLAKYVRRSQAYQSAALKSYVKDQMWDMFPAL
                     RPYRDVLLQGKYLGWKAPADAGAARVVLNYTLIDMLAYVATGKMSPEESLKWGEAQLK
                     GIYGT"
     gene            322..1707
                     /locus_tag="VNU03_01010"
     CDS             322..1707
                     /locus_tag="VNU03_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73053.1"
                     /translation="MTRSRRRGVTSLILVLVIATCARTAGAQDVSISGTITVAPAAKL
                     KLPKELLLIIRVSKTSDTKKAPIAVKRVPAPEFPYRYTLGEEDITLDGSRLEGKLYVT
                     ARVEPGDGGGTPAGSLEGDYPRNPVSVGAKGVDITIGVAVPPQTVEAPAGSLKPRDAG
                     IVRIGLLWSGSTPFGNSSVPEELRLAFRDLGYVDDRNIAFEARYAEGRYDRLPALAAN
                     LVDLKVDVILAAGDSAAILAAKHATGKVPIVMMALADTVQLGLVPSLARPAGNLTGLS
                     FPLGAMAGKQLELLKKAIPSLRRVGVLWNPANPGHAPVLEKLTAAALQLELKLQLIEV
                     RGPDDFETAVTTLKRSRADGLLVLWDPMFYAHGGRLTLLALRDHLPTISTYREFAEAA
                     GLMTYGPSLADIFRGAASYVDKIVRGGKPADLPVEQPLRFELVLNLATAKALGVTLPE
                     SILVRADRVLQ"
     gene            1704..>2624
                     /locus_tag="VNU03_01015"
     CDS             1704..>2624
                     /locus_tag="VNU03_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HWP73054.1"
                     /translation="MRRGRSLFRKYALLLVALLGVALLANSLLEMYSSYQANKGALLA
                     IQKGEASVAAARIEQFVSEVERQVTLVAESQAVRSSDLDFARMLRHVAAVTDVSYLDG
                     AGREQLRVSRLDLDVRNSGDDHSRERRFLSPKLGTTYFSPVYFRDGSEPYMTVAVAGS
                     GLGSGVVAAEVNLKFIREVVSRIHVGKAGHAYVVDTSGTLIAHRNISMVLRQTSFSGL
                     PHVGAALAGRTQPDAQEPATVGTDVDGRRVLTAHAPIEPLDWLVFLERPLDEAFEPLY
                     ASIVRSVSLLAGGLVLSVVASLVLARKIVGP"
BASE COUNT          394 a          910 c          906 g          414 t
ORIGIN      
        1 cgcgaaggaa ttcctgacct ggctcatgga gaaggaacag ctggcaaagt acgtgcgccg
       61 gagccaggcc tatcagagcg cagccctgaa gagctacgtg aaggaccaga tgtgggacat
      121 gttcccggcg ctgcggccct atcgcgacgt gctgctgcag gggaaatacc tgggctggaa
      181 ggctcccgcc gatgcggggg cggcgcgagt ggtcctcaac tacaccctca tcgacatgct
      241 cgcctacgtg gcgacgggga agatgtcccc cgaggagtcc ctcaagtggg gcgaggcgca
      301 gctgaagggg atctacggca cgtgacgcgg agccggcgtc gcggcgtcac gtctctgatc
      361 ctggtgctgg tgatcgcgac gtgcgcgcga acagccgggg cgcaggacgt ctcgatctcc
      421 ggcacgatca cggtggcgcc ggccgcgaag ctgaagctgc cgaaggagct cctcctgatc
      481 atccgggttt ccaagacgtc ggatacgaag aaggcgccga tcgcggtcaa gcgcgtcccg
      541 gctcccgagt tcccgtaccg gtacacgctc ggcgaggagg acatcacgct cgacggctcg
      601 cgcctcgagg gcaagctcta cgtcaccgcg cgcgtcgagc ccggggacgg gggcggaacg
      661 ccggccggat cactcgaggg cgactatccg cgaaacccgg tctcggtcgg ggcgaaagga
      721 gtcgacatca cgatcggcgt cgcggttccg cctcagaccg tggaggcgcc cgccggctcg
      781 ctcaagccgc gcgatgccgg gatagtccgg atcggtctct tgtggtcggg ctcgaccccg
      841 ttcgggaatt cgtcggtgcc cgaagagctg cggctcgcgt tccgtgacct cggctatgtc
      901 gacgaccgga acatcgcgtt cgaggctcga tacgcggaag ggcgctacga ccggctcccc
      961 gcgctcgcgg cgaacctcgt cgacctcaag gtcgacgtga tcctggccgc cggggactcg
     1021 gccgcgatcc tggccgccaa gcatgcgacc ggcaaggtcc cgatcgtgat gatggctctg
     1081 gccgacaccg tccagctcgg cctcgtgccc agcctggcgc ggccggccgg caatctcacc
     1141 ggcctctcgt tcccgctcgg cgcgatggcc ggcaagcagc tggagctgct caagaaggcg
     1201 atcccgtcgc tgcggcgggt cggagtcctg tggaatcccg ccaaccccgg tcacgccccg
     1261 gttctggaga agctcaccgc ggccgcgctc cagctggagc tcaagctcca gctcatcgag
     1321 gtccgggggc cggacgattt cgaaacggcg gtgaccacgc tgaaacggtc gcgggccgat
     1381 ggtctgctcg tgctctggga tccgatgttc tacgctcacg ggggccggct cacgctgctc
     1441 gcgttgcggg accacttgcc gacgatttcc acctaccggg aattcgcgga ggcggccggg
     1501 ctcatgacgt acggccccag cctggcggac atcttccgcg gcgctgcatc gtacgtcgac
     1561 aagatcgtcc ggggcgggaa gccggcggac ctgccggtcg agcagcccct ccggttcgag
     1621 ctcgtcctca acctcgccac cgcgaaggcc ctcggggtga ccctcccgga atcgatcctg
     1681 gtgcgcgcgg accgggttct gcagtgaggc gcggccgtag cctgttccgc aagtacgcgc
     1741 tcctcctggt cgcgctgctc ggagttgcgc tcctggccaa cagcctgctc gagatgtact
     1801 cctcctacca ggcgaacaag ggcgctctcc tcgcgatcca gaagggcgag gcctccgtcg
     1861 cggccgcccg catcgagcag ttcgtgtcgg aggtggagcg ccaggtgacg ctcgtggccg
     1921 agtcacaggc ggtgcgctcg tcggacctgg acttcgcccg catgctccgt cacgtggccg
     1981 cggtgaccga cgtgagttac ctcgacggcg ccggccgcga gcagctccgc gtgtcccgcc
     2041 tcgatctcga cgtccggaac agcggcgacg atcactcccg cgagcgccgg ttcctcagcc
     2101 cgaagctcgg caccacctac ttcagcccgg tctacttccg cgacgggtcc gagccctaca
     2161 tgacggtggc cgtcgcgggc agcggtctgg ggtccggcgt ggtcgccgcg gaagtgaacc
     2221 tgaagttcat ccgggaggtg gtctcgcgga tccacgtcgg gaaggctggc cacgcctacg
     2281 tggtggacac gtccgggacg ctcatcgccc accggaacat cagcatggtg ctacgccaga
     2341 cgagcttctc cggcctcccg cacgtcggcg ccgcgctggc cggccggacg cagccggacg
     2401 cgcaggagcc ggcgaccgtc gggaccgacg tcgacggtcg ccgggtcctg accgctcacg
     2461 ccccgatcga gccgctcgac tggctcgtct tcctggagcg gccgctggac gaggcgttcg
     2521 agcctctcta cgcctccatc gtgcggagcg tgtcgctgct cgccggcggc ctggtgctct
     2581 cggtggtggc gagcctcgtc ctcgcgcgga agatcgtcgg cccc
//
LOCUS       DATMCV010000039         4802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_799549, whole genome shotgun sequence.
ACCESSION   DATMCV010000039 DATMCV010000000
VERSION     DATMCV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799549"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /gene="arsM"
                     /locus_tag="VNU03_01020"
     CDS             <1..625
                     /gene="arsM"
                     /locus_tag="VNU03_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HWP73055.1"
                     /translation="GNPTALAELRAGETVLDLGSGGGIDVLLSAGRVGPTGRAYGLDM
                     TDEMLALARENQRRAGATNVEFLKGEIEQIPLPSGSVDVIISNCVINLSADKDRVLAE
                     AFRVLKPGGRLAVSDVVVRGEIPSAIRRSVELWIGCVAGALEEREYRDKLARAGFEAI
                     DLEPTRIYRVEDARDFLEGAGLDVDAIAPQVDDKFMSAFVRARKPAR"
     gene            664..1851
                     /locus_tag="VNU03_01025"
     CDS             664..1851
                     /locus_tag="VNU03_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73056.1"
                     /translation="MCAQIGAYTWPALLPGFIAEWHLTNREAGWITGIFYAAYTLSVP
                     ILVTLTDRVDARKVYLSGVALTIASHLGFAALADGFWSAAVARALAGIGWAGTYMTGL
                     KLLADRVEPALMSRAVAGHAASIGIAGALSFVFAGGLGQWVGWRGAFAAAGLAALAAW
                     LIVFVWAPSRARATVGAPAGRLFDFRPVLRNRSAMAYALAYCAHTWEMNSLRGWAVAF
                     LAYVVATTDAGRPWIAPAVVAMTMGLVGTWASVMGNEASIRLGRQRLVRLAMTASLVC
                     AAGIGFVGVRSYALAAALVLVYGALIWLDSSSLTAGAAGSADPARRGATLALHSMAGY
                     AGGFVGPIAIGWILDLAGGMSPLGWGLAFLHVAVIGLLGQIVFVALAPRDLAGDRSRV
                     PGR"
     gene            complement(1865..2347)
                     /locus_tag="VNU03_01030"
     CDS             complement(1865..2347)
                     /locus_tag="VNU03_01030"
                     /inference="COORDINATES: protein motif:HMM:NF020413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
                     /protein_id="HWP73057.1"
                     /translation="MARLDRFRDAQADTHSGFAAALGELEAGRKTGHWIWYVFPQLVG
                     LGQSMTAVHYGLADAAEAADYLRDPVLGERLVAAAAAARRHLTGSPRVGVVTLMGSVV
                     DALKLVSCMTLFAHVAAVLAGEDPTPRFTAMAEHARAILAAAAASGYDRCAFTEATLR
                     "
     gene            2406..3257
                     /locus_tag="VNU03_01035"
     CDS             2406..3257
                     /locus_tag="VNU03_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWP73058.1"
                     /translation="MKPAPFRYARPATAAEAVALLASGGGDAKLLAGGQSLVPLLNMR
                     LVRPAVLVDLNGARELERIDPAPDGGLVLGSLVRHADLAAAPLVRERAPLLAEAARHV
                     GHTAIRNRGTLGGSLAHADPAAELPAALLALDATLGLLGPRGLRRVAAGDFFRGLLVT
                     ALAEDELLAEVTVPAAPRGWGFVEIARRPGDFALAGVAASLDGKGHARVVGFGVADRP
                     VRLRAAEALVAAAPRDPATPARAGAAAAADCDPATDVHASAEYRRHLATVLTEDALRQ
                     AVGRLGS"
     gene            3254..3763
                     /locus_tag="VNU03_01040"
     CDS             3254..3763
                     /locus_tag="VNU03_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWP73059.1"
                     /translation="MTAGAPPAPAERLRVRLAVNGAPVERVVEARRSLADFLREDLAL
                     TGTHVGCEHGVCGACTVLIDGRSARSCLLLAVQLEGARVTTIEGLTPPDGLSPLQEAF
                     RACHAMQCGFCTPGMIVASTELLAATPAPDEAAIREALAGNLCMCTGYVNIVRAVAQA
                     AARPAVPAP"
     gene            3760..>4802
                     /locus_tag="VNU03_01045"
     CDS             3760..>4802
                     /locus_tag="VNU03_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP73060.1"
                     /translation="MSEAARGWVGVALPLKEDARLVAGRGRFLDDLGRPHLLHAAMLR
                     SPHAHARIMALDATAAAAMPGVLAVLTGAEAVVRSGPIRPLIPTTAAVPDHCLAVDRV
                     RYVGEPVAAVAAVDRATAEDALERIRVEYAPLPAVVDPEAAMRADAPLLYPALGTNVV
                     WHDTLTYGDVDGALARADGVLRERFTIQRYASTPLETFGAIAEYDAGTDAFEFWTNDQ
                     RPGLTISILAASLGVPQSRIRLHCPDIGGAFGNKRRPAYLLVCALLARAAGRPVKWIE
                     DRIENLTALMHACNGIMDVALAYRADGTLLALDVRDVADEGKNLLTPTQHNLIKLGNI
                     ANGYRIPAVRYAA"
BASE COUNT          550 a         1821 c         1772 g          659 t
ORIGIN      
        1 cggcaatccc accgccctcg cggagctccg ggcgggcgag acggtgctcg atctggggtc
       61 cggaggcggc atcgacgttc tcctgtccgc gggtcgggtg gggccgaccg gccgcgccta
      121 cgggctcgac atgaccgacg agatgctggc cctcgcccgg gagaaccagc gccgggccgg
      181 ggccaccaac gtggagttcc tcaagggcga gatcgagcag atcccgctcc cgtcgggctc
      241 ggttgacgtc atcatctcca actgcgtgat caatctctcg gccgacaagg accgggtgct
      301 cgcggaggcg ttccgcgtgc tcaagcccgg cgggcgtctg gcggtgtcgg acgtcgtcgt
      361 ccgcggcgag atcccgtctg cgatccgccg cagcgtcgag ctctggatcg gctgcgtggc
      421 cggcgcgctc gaggagcggg agtaccgcga caagctcgcc cgcgcgggct tcgaggccat
      481 cgatctcgag ccgacgcgga tctaccgggt ggaggacgca cgggacttcc tggagggagc
      541 cgggctcgac gtggacgcga tcgctcccca ggtggacgac aagttcatga gcgcgttcgt
      601 gcgggcccgg aagccggcgc gctgaccccg gcccgcctcg tcaccgccgt ctgcgcggcc
      661 caggtgtgcg cccagatcgg cgcctacacg tggcccgccc tgctgcccgg gttcatcgcg
      721 gagtggcatc tcacaaaccg ggaagcgggc tggatcaccg ggatcttcta cgctgcgtac
      781 acgctgtcgg ttcccatcct ggtcaccctg accgatcgcg tggatgcccg aaaggtctac
      841 ctctccggcg tcgccctgac catcgcgagt cacctcggtt tcgccgccct cgccgacggg
      901 ttctggagcg cggcggtggc gcgggcgctg gccgggatcg ggtgggccgg cacctacatg
      961 accgggctca agctgctggc agaccgggtc gagcccgcgc tgatgtcccg ggcggtggcc
     1021 ggacacgcgg ccagcatcgg catcgcgggc gcgctctcgt tcgtgttcgc gggcgggctc
     1081 ggccagtggg tcggatggcg cggcgcgttc gccgcggccg ggctcgccgc gctcgcggcc
     1141 tggctcatcg tgttcgtctg ggccccgtcg cgggcgcgcg cgaccgtcgg ggcgccggcc
     1201 ggtcggctct tcgacttccg gccggtcctg cgtaaccgat ccgccatggc gtacgcgctg
     1261 gcctattgcg cccacacctg ggagatgaac tccctgcggg gctgggcggt cgcgttcctg
     1321 gcctacgtgg tggcgacgac cgacgccggc cgcccgtgga tcgcgccggc ggtggtggcg
     1381 atgaccatgg ggctcgtcgg cacgtgggcc agcgtgatgg gcaacgaggc gtcgatccgc
     1441 ctcggccgcc agcggctggt gcgcctggcc atgaccgcgt ccctcgtctg cgcggcgggc
     1501 atcggcttcg tcggcgtgcg ctcctacgcg ctcgcggccg cgctcgtgct cgtgtacggc
     1561 gcgctgatct ggctcgactc ctcctcgctc accgcgggcg cggcgggcag cgccgatccc
     1621 gcgcggcgcg gcgccacgct ggcgctgcac tcgatggccg gctacgcggg cggcttcgtc
     1681 gggccgatcg cgatcggctg gatcctcgat ctcgcgggtg gcatgtcgcc gctggggtgg
     1741 ggcctcgcct tcctgcacgt ggcggtgatc gggctcctcg ggcagatcgt gttcgtcgcc
     1801 ctggctcccc gcgacctggc gggcgaccgc agccgggtac ccgggcgttg acgcggctcg
     1861 gcgatcaccg aagggtcgcc tccgtgaacg cgcagcgatc gtagccgctg gccgccgcgg
     1921 cggccaggat ggcccgggcg tgctcggcca tcgcggtgaa ccgcggggtg ggatcctcgc
     1981 ccgcgagcac cgccgccacg tgcgcgaaca gggtcatgca cgagacgagc ttgagcgcgt
     2041 cgacgaccga gcccatgagg gtgacgacgc cgacccgggg agagccggtc agatgtctcc
     2101 gggctgcggc cgccgccgcg accagccgct cgccgagcac cggatcgcgt aggtagtcgg
     2161 ccgcctccgc cgcgtcggcg agaccgtagt ggaccgcggt catcgactgg cccaggccca
     2221 cgagctgagg gaagacgtac cagatccagt ggccggtctt ccgcccggcc tcgagctcgc
     2281 ccagagccgc cgcgaatccg gagtgggtat ccgcctgcgc gtcgcggaag cgatccagcc
     2341 gcgccatggg gcaggatggc gcgctcggcg ccggcgcgca agggccgtgg cataatcgcg
     2401 tcgcgatgaa gcccgctccc ttccgctacg cgcgtcccgc caccgccgcg gaggcggtcg
     2461 cccttctcgc gtcgggcggg ggcgacgcga agctgctcgc gggcggccag agcctcgtgc
     2521 cgctgctcaa catgcgcctc gtccgtccgg cggtgctcgt cgacctgaac ggcgcgcgcg
     2581 agctcgagcg gatcgatccc gcgccggacg gagggctcgt cctggggtcg ctggtccgcc
     2641 acgccgatct cgcggccgcg ccgctcgtgc gcgagcgggc gccgctcctg gccgaggcgg
     2701 cgcgccacgt gggccacacc gcgatccgca atcgcggcac tctcggcggc agcctcgccc
     2761 acgccgatcc ggccgcggag ctgcccgccg cgctcctcgc gctcgacgcc acgctcggcc
     2821 tgctgggacc ccgcgggctg cgccgggtgg cggccggcga cttcttccgc ggcctcctgg
     2881 tcaccgcgct cgccgaggac gagctgctcg ccgaggtcac ggtgcccgcg gccccgcggg
     2941 gctggggctt cgtggagatc gcgcgccgcc ccggcgactt cgcgctggcc ggcgtggcgg
     3001 cctccctcga cgggaagggg cacgcccgcg tggtgggatt cggcgtggcc gaccggccgg
     3061 tccgtctgcg cgcggccgag gcgctggtgg ccgcggcgcc gcgcgatccg gcgacgccgg
     3121 cccgggcggg ggcggccgcg gcggcggact gcgatccggc caccgacgtc cacgcctcgg
     3181 cggagtatcg ccgccacctc gccaccgtgc tgaccgagga cgccctgcgc caggcggtcg
     3241 ggcgcctcgg atcatgacgg ccggcgcccc gcccgcaccg gccgagcgac tgcgggtgcg
     3301 tctcgccgtc aacggtgcgc cggtggagcg cgtggtggag gcgcgccgct cgctcgcgga
     3361 cttcctccgg gaagacctcg cgctcaccgg cacccacgtg ggctgcgagc acggcgtctg
     3421 cggcgcgtgc acggtgctga tcgacgggcg cagcgcccgg agctgcctgc tcctggccgt
     3481 ccagctcgag ggcgcgcgcg tcaccaccat cgagggcctc actccgccgg acggcctcag
     3541 cccgctccag gaggcgttcc gggcgtgcca cgcgatgcag tgcggcttct gcacgcccgg
     3601 catgatcgtg gcgagcaccg agctgctcgc ggcgaccccc gcgcccgacg aggcggccat
     3661 ccgcgaggcg ctcgccggga acctctgcat gtgcaccggc tacgtcaaca tcgtccgcgc
     3721 ggtcgcgcag gccgccgcgc ggcccgcggt cccggcgccg tgagcgaggc cgcccggggc
     3781 tgggttggcg tcgccctgcc cctcaaggag gacgcgcgcc tggtcgccgg gcgcggccgg
     3841 ttcctcgacg acctcggccg cccgcacctg ctccacgcgg ccatgctccg gagcccgcac
     3901 gcccacgccc gcatcatggc gctcgacgcg accgcggccg ccgcgatgcc cggcgtcctg
     3961 gccgtgctca ccggcgcgga ggcggtcgtg cgctcgggcc cgatccgccc gctcatcccc
     4021 accaccgccg cggtgcccga ccactgcctg gcggtggacc gcgtgcgcta cgtgggcgag
     4081 ccggtggcgg cggtggcggc ggtggaccgc gccaccgcgg aggacgcgct cgagcgcatc
     4141 cgcgtcgagt acgcgccgct ccccgcggtc gtcgacccgg aagccgccat gcgcgcggac
     4201 gccccgctgc tctatcccgc gctcgggacc aacgtggtct ggcacgacac gctcacctac
     4261 ggcgacgtgg acggcgcgct ggcccgtgcc gacggggtgc tgcgggagcg cttcaccatc
     4321 cagcgctacg cctccacccc gctcgagacc ttcggagcca tcgcggagta cgacgcgggc
     4381 accgacgcct tcgagttctg gaccaacgac cagcgcccgg ggctgaccat ctcgatcctg
     4441 gccgcctcgc tcggcgtccc gcagtcgcgg atccgcctgc actgtcccga catcggcggc
     4501 gccttcggca acaagcgccg gccggcttat ctcctggtgt gcgcgctgct ggcccgcgcg
     4561 gccgggcggc cggtgaagtg gatcgaggac cgcatcgaga acctcaccgc gctcatgcac
     4621 gcgtgcaacg ggatcatgga cgtcgcgctc gcctaccggg ccgacggcac cctgctcgcg
     4681 ctcgacgtcc gcgacgtcgc ggacgagggc aagaacctgc tcacgccgac ccagcacaac
     4741 ctgatcaagc tcggcaacat cgccaacggc taccggatcc ccgcggtgcg atacgcagcg
     4801 tg
//
LOCUS       DATMCV010000040         2816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_334447, whole genome shotgun sequence.
ACCESSION   DATMCV010000040 DATMCV010000000
VERSION     DATMCV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334447"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VNU03_01050"
     CDS             complement(<1..833)
                     /locus_tag="VNU03_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWP73061.1"
                     /translation="MPDLLAARSQMAMSLGFHILFAMVGVAMPVLMVVAERRWQRTGD
                     HIYLDLAHRWAKGTAILFAVGAVSGTVLSFELGLLWPAFMEQAGAIIGMPFSLEGFAF
                     FTEAVFLGVYLYGWDRISPRAHLAAGWAVAVSGMLSAVFVVIANAWMNSPTGFRWVGG
                     RAIDIDPIAAMLNPAAFSQVIHMTLAAYASTGLVVAGIHALPLLRQPRNAFHRRALSV
                     ALLIGAPAALLQPLAGDQSARFVAHYQPVKLAALEGHFKTERGAPLRIGGWPDAAAGK
                     TR"
     gene            1003..1521
                     /locus_tag="VNU03_01055"
     CDS             1003..1521
                     /locus_tag="VNU03_01055"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HWP73062.1"
                     /translation="MGVMLVVLVEACASPQRPTSAVAVPEKLNPGAGESLAMIVAARG
                     VQIYECRPRKDQAGVYEWAFAAPEADLFDGRGRKVGRHYAGPHWEAVDGSKILGTVRE
                     RVDAPVAGAIPWLLLTATSVGPDGAFSRITSVQRVNTRGGAPPTTDCSQATAGTPARI
                     EYTADYDFFTAR"
     gene            1584..1874
                     /locus_tag="VNU03_01060"
     CDS             1584..1874
                     /locus_tag="VNU03_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HWP73063.1"
                     /translation="MCRSIKTLHNFKPPATDEEIRASSLQFVRKLSGFSKPSKANEVA
                     FSRAVDEVARAARALLDALVTNAPPRDREAEASKARARGADRFRSRDTRQAS"
     gene            1889..2410
                     /locus_tag="VNU03_01065"
     CDS             1889..2410
                     /locus_tag="VNU03_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73064.1"
                     /translation="MRRQLATALSLLGVLWGVSGCATAVDPADVKLADFKSDGCSLFP
                     DGTYYSCCYLHDVTYWPSGTADEREKADKALRACVVDVTQNAALAEAMYHGVRVGGGP
                     ELPTSYRWGYGWPFPYRKDYAPLNGAERAQVVEKTQKLCSTMHVNPSTGGVVVDVGRE
                     ISLAQARQICPGL"
     gene            2448..>2816
                     /locus_tag="VNU03_01070"
     CDS             2448..>2816
                     /locus_tag="VNU03_01070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP73065.1"
                     /translation="MIRPLREADAESYVTLRRQGLLEAPLAFGASPEDDIAASAEAVR
                     DRLRQGPEWVILGAFEDGLVGAVGLMRDRYVKASHKAHVWGMYVAPGQRGRGVGADLL
                     QATLQHARGLPGVSWVHLAVT"
BASE COUNT          456 a          922 c         1045 g          393 t
ORIGIN      
        1 cgcgtcttgc ccgcggccgc gtcgggccag ccgccgatcc gcaggggcgc gccccgctcg
       61 gtcttgaagt gcccctcgag ggccgccagc ttgaccggct ggtagtgcgc cacgaagcgc
      121 gcgctctggt cgcccgccag cggctggagc agggccgccg gcgcgccgat cagcagcgcc
      181 accgagagcg cgcggcgatg gaaggcgttc cggggctgac ggagcagggg cagcgcgtgg
      241 atgcccgcca ccaccaggcc ggtggaggcg taggccgcga gcgtcatgtg gatcacctgg
      301 gagaaggcgg ccgggttcag catcgccgcg atcgggtcga tgtcgatcgc tcgcccgccc
      361 acccaccgga acccggtggg cgagttcatc caggcgttgg cgatgaccac gaagaccgcg
      421 gagagcatgc cgctcaccgc caccgcccag cccgcggcca ggtgcgcgcg cggggagatg
      481 cgatcccagc cgtagaggta gaccccgagg aagacggcct cggtgaagaa ggcgaagccc
      541 tcgagcgaga agggcatgcc gatgatggca cctgcctgct ccatgaaggc aggccagagc
      601 agccccagct cgaagctcag cacggtgccg gagaccgccc cgaccgcgaa gaggatcgcg
      661 gtgcccttgg cccagcggtg cgcaagatcg aggtagatgt gatcgccggt gcgctgccag
      721 cgccgctcgg ccaccaccat gagcaccggc atggccacgc cgaccatcgc gaagaggatg
      781 tggaacccca gcgacatggc catctgcgag cgcgcggcga ggagatcggg catggctccg
      841 tggccatggt agcccgccgg cgcgcgggcc gcattcgcgg gttcttgctc atcacgcgga
      901 aaagctgtgc tgtaaggaca ccctcacgct tgacaccccc cgacgagcac gccactctct
      961 gtccacgtga cggagggcgc gatggtgact gcacgggggc tgatgggcgt catgctggtg
     1021 gttctcgtcg aggcgtgcgc gagcccgcaa cgcccgacgt ccgcggtcgc ggtgccggag
     1081 aagctcaatc ccggcgcggg cgaatcgctc gcgatgatcg tcgcggcaag gggggtgcag
     1141 atctacgaat gccgaccccg gaaggatcaa gccggggtgt acgagtgggc gttcgcggct
     1201 cccgaggccg atctcttcga cggccggggc cggaaggtcg gccgccacta tgccgggccg
     1261 cactgggagg cggtcgatgg gagcaagatc ctcggaacgg tgagggaacg ggtcgacgcc
     1321 ccggtggccg gggcgatccc gtggctgctg ctcaccgcaa cgtcggtggg ccctgacggc
     1381 gcgttcagcc ggatcacgag cgtccagcga gtgaacacca ggggtggcgc gccgcccacg
     1441 accgactgtt ctcaggcgac cgccgggacg ccggcccgga tcgagtacac ggcggactac
     1501 gatttcttta ccgccaggta gactgactct gtctcgggcg gatcgcgtgg ccaatcatta
     1561 ctgaataacc aggaggccct ggcatgtgcc gaagcatcaa gaccctgcac aacttcaagc
     1621 cgccggccac ggacgaggag atccgggcat cgtcgctgca gttcgtccgg aagttgagcg
     1681 gcttctcgaa gccgtccaag gcgaacgagg tcgccttctc ccgggcggtg gacgaggtcg
     1741 cgcgggcggc gcgcgccctt ctcgatgctc tcgtgacgaa cgctccgcct cgcgatcgcg
     1801 aggccgaagc gtcgaaggcc cgcgcgaggg gcgcggaccg gtttcgctcc cgggatacga
     1861 gacaggcctc gtgacgtggg gagagagggt gcgacgacag ctggccaccg ccctgagtct
     1921 gctgggagtc ttgtgggggg tgtccgggtg cgccacggcc gtcgaccccg ccgacgtcaa
     1981 gctcgccgac ttcaagagtg acgggtgcag cttgttcccc gacggaacct actacagctg
     2041 ctgctacctc cacgacgtca cgtactggcc gagcgggacg gccgacgagc gcgagaaggc
     2101 ggacaaggcg ctgcgcgcgt gcgttgtgga cgtcacccag aacgcggccc tggcggaggc
     2161 aatgtaccac ggcgtgcgtg tcgggggtgg cccggagctg cccacctcgt atcggtgggg
     2221 ctacggctgg ccctttccct atcgcaagga ctatgcgccg ctcaacggcg cggagcgggc
     2281 gcaggtcgtc gagaagacgc agaagctctg cagcacgatg cacgtgaatc cctccaccgg
     2341 aggggtcgtg gtggacgtcg gccgggagat cagcctcgcg caggcgaggc agatctgccc
     2401 gggtctgtga cggacccgga ccacgcgtga tggctggcgg cgccagggtg atcaggccgc
     2461 tgcgggaggc cgacgccgag tcgtatgtca cgctccggag gcaagggctc ctggaggcgc
     2521 cactcgcctt tggcgcatct cccgaggacg acatcgccgc ctcggcggag gcggtacgcg
     2581 accggcttcg gcaggggccg gaatgggtga tcctgggggc gttcgaggac gggctggtcg
     2641 gggcggtggg cctcatgcgc gatcggtacg tgaaggcctc ccacaaagct cacgtctggg
     2701 ggatgtacgt ggcgccaggc cagcgcggcc gcggtgtcgg ggccgatctg ctccaggcca
     2761 cgctccagca cgcgcgagga ttgcccggtg tgtcgtgggt gcatctcgcc gtcacg
//
LOCUS       DATMCV010000041         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_666725, whole genome shotgun sequence.
ACCESSION   DATMCV010000041 DATMCV010000000
VERSION     DATMCV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666725"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1163)
                     /locus_tag="VNU03_01075"
     CDS             complement(9..1163)
                     /locus_tag="VNU03_01075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWP73066.1"
                     /translation="MSRASERRPAGPIEARLDWLDGTKGIAILWIAFFHFFIAYNGGR
                     LPWLLDPGYLASYERICAPESPAARALCVGSAVLTAAVQLGFNGVAVFLVLSGFGLSY
                     ALARHGRPPTAWAGWYRSRLVRLLPMYWVAHLVYLVSPFQARLDPLDYRFALSFLGDR
                     VLPIETMFYYFNPALWYFGLLLQLYLVFPVLFVALERLGAARFLVAAAVVTFGARWVL
                     LFAMPMDGRWVQGGFFACRLWEFALGMAAGALWRKWPAAIDAAVLSGRGLLAGSALYA
                     LGLYSYGALWSFLFTDALIGTGLTVVLVNVARVAGRIPGVGAAALTVGTSSYGLYLVH
                     QPYVQYFGERMRSLDVAVFVVGAAGIIAALALGAMALERAVNSLTRRVLG"
     gene            1253..1924
                     /locus_tag="VNU03_01080"
     CDS             1253..1924
                     /locus_tag="VNU03_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWP73067.1"
                     /translation="MPDLLDAVEIESAPSPTAAVIWMHGLGADGHDFEPIVPELRLPA
                     RLPVRFVFPHAPLRPVTINQGHVMRAWYDIRALAGARREDEAGVRQSARQLEALVERE
                     RQRGIAPGRIVLAGFSQGGAMALHVGLRYTERLAGIMALSCYLPLSDVLATEAAPANR
                     DVPIFWAHGVHDPMIPLAMAELGHRQVAGLGYSIEWHQYAIPHSVSPEEIADVARWLG
                     RVLGP"
     gene            1952..2575
                     /locus_tag="VNU03_01085"
     CDS             1952..2575
                     /locus_tag="VNU03_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWP73068.1"
                     /translation="MQTDRLKMPIGEAMFTQRSTRKLRPDPIPVEDLHLLIEAAVKAP
                     NGGNNQVGRFLIVNDRAKIREFGALYKEAWWAKRWDDHKWTKPEDIPLTDKNHRSAMG
                     LSEDMKDVPCVVFAFTAPGGGANSVIPACQNLMLAAHALGIGSLPTTLHAKVMDRFHA
                     MFDIPKEMAFHFCIPLGYPAVKYGPSKRKPTTEITSLNRWGGAVPWA"
     gene            2566..>3015
                     /locus_tag="VNU03_01090"
     CDS             2566..>3015
                     /locus_tag="VNU03_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP73069.1"
                     /translation="MGVTRKPAVSLAAVPGRRKATVDMAKRLEEEGFTGIYCPSLGDG
                     LGLCEAIALATRTIPFGTSISNIYTRHALDFAQTAALIHELAGGRFRFGVGISHGPTH
                     DRLGIKPGKPLEDIRRFVQDWKSGAKLAGELPPITLATLRKKMTALSA"
BASE COUNT          523 a         1105 c         1018 g          369 t
ORIGIN      
        1 gccgccggct agccgagcac ccgtctcgtc aagctattca ccgcgcgctc gagcgccatc
       61 gctccgagcg cgagagccgc gatgatcccg gccgcgccca ccacgaacac cgccacatcg
      121 agcgagcgca tccgctcgcc gaagtactgc acgtagggct gatggacgag gtagagcccg
      181 tatgaagacg tcccgaccgt cagcgcggcg gcccccaccc ccggaatgcg gccggccact
      241 cgcgcgacgt tgacgaggac gacggtcagg ccggtgccga tgagcgcatc ggtgaagaga
      301 aaggaccaga gcgcgccgta cgagtagagg ccgagggcat agagcgcgct cccggccagc
      361 aggcctcgcc ccgacaggac cgcggcgtcg atcgcggccg gccacttccg ccagagggcg
      421 cccgcggcca tgccgagggc gaactcccag agtcggcacg cgaagaagcc gccctgcacc
      481 catctcccgt ccatcggcat ggcgaacagg agcacccagc gggcgccgaa ggtgacgacc
      541 gccgcggcga cgaggaaccg ggccgcgccg aggcgctcga gcgcgacgaa gagcacggga
      601 aagaccaggt agagctgcag gagcaggccg aagtaccaga gcgcggggtt gaagtagtag
      661 aacatcgtct cgatcggcag cacccggtcg ccgaggaagc tcagcgcgaa gcggtagtcg
      721 agcgggtcca gccgggcctg aaacggcgac accaggtaga cgaggtgcgc gacccagtac
      781 atcggcagca ggcgaaccag gcggctccgg taccacccgg cccaggccgt cggcgggcga
      841 ccatgccggg cgagcgcata cgagagcccg aacccggaga gaacgaggaa gaccgcgacc
      901 ccgttgaacc cgagctggac cgccgcggtg agcaccgccg acccgacgca gagggcccgc
      961 gcggcgggag actccggggc gcagatcctc tcgtacgacg cgaggtagcc gggatcgagc
     1021 agccacggca gccggccgcc gttgtacgcg atgaagaagt ggaagaacgc gatccagagg
     1081 atcgcgatcc ccttcgtccc atcgagccag tcgagccgcg cctcgatcgg gcccgccggg
     1141 cgtcgctcgc tcgcgcggct cacgaccgcg acgatagggg tggggcgatc gcgtgtcaat
     1201 tgcgccggac gccccgatcc gacgtatact gccgaccacg accacgcccg ccatgcccga
     1261 cctcctggac gccgtcgaga tcgagagcgc gccgagcccc accgcggcgg tgatctggat
     1321 gcacggcctc ggcgccgacg gccacgactt cgagcccatc gtgcccgagc tgcggctgcc
     1381 cgcacgcctg ccggtccggt tcgtcttccc gcacgctccc ctccggccgg tcaccatcaa
     1441 ccagggccac gtcatgcgcg cgtggtacga catccgcgcc ctcgcggggg cgcgccgcga
     1501 ggacgaggcg ggcgtccgcc agtccgcgcg ccagctcgag gcgctggtgg agcgcgagcg
     1561 gcagcgcggg atcgcgcccg gccggatcgt gctggccggc ttctcccagg gcggcgcgat
     1621 ggcgctgcac gtcgggctgc gctacaccga gcgcctcgcc gggatcatgg cactctcgtg
     1681 ctacctgccg ctgtccgacg tcctggccac cgaggcggcg cccgccaacc gggacgtgcc
     1741 gatcttctgg gcccacggcg tccacgatcc gatgatcccg ctggcgatgg ccgagctcgg
     1801 gcaccggcag gtcgccggcc tcggctactc gatcgagtgg caccagtacg ccatccccca
     1861 ttccgtctcc cccgaggaga tcgccgacgt cgcccgctgg ctcgggcggg tgctcggccc
     1921 gtgaccctct cgcccatcag gaggtgagcc catgcagacc gaccgtctga agatgccgat
     1981 cggggaagcg atgttcaccc agcggtccac ccggaagctc cggcccgatc cgatcccggt
     2041 ggaggacctc cacctcctga tcgaggccgc ggtcaaggcg ccgaacgggg gcaacaacca
     2101 ggtaggacgc ttcctcatcg tcaacgaccg cgcgaagatc cgcgagttcg gcgcgctcta
     2161 caaggaagcg tggtgggcca agcgctggga cgaccacaag tggacgaagc ccgaggacat
     2221 cccgctcacc gacaagaacc accgctccgc gatggggctg tcggaggaca tgaaggacgt
     2281 gccttgtgtg gtattcgcgt tcaccgcgcc cggcggcggc gccaactcgg tgatccccgc
     2341 gtgccagaac ctgatgctgg ccgcgcacgc cctcggcatc ggctcgctgc ccaccacgct
     2401 gcacgccaag gtcatggacc gcttccacgc gatgttcgat atcccgaagg agatggcctt
     2461 ccacttctgc atcccgctcg gctacccggc ggtgaagtac gggccgagca agcggaagcc
     2521 gacgacggag atcacctcgc tcaatcgctg gggaggagcg gtgccgtggg cgtgacgcgc
     2581 aagcccgcgg tcagcctcgc cgcggtcccc ggccgccgca aggccaccgt ggacatggcg
     2641 aagcgcctcg aagaggaggg cttcaccggt atctactgtc cgagcctggg cgacggcctc
     2701 ggcctgtgtg aggccatcgc cctcgcgacc cgcacgatcc ccttcggcac cagcatctcc
     2761 aacatctaca cgcggcacgc cctcgacttc gcccagaccg cggccttgat ccacgagctg
     2821 gcgggcgggc gctttcgctt cggggtcggc atcagtcacg ggcccaccca cgaccgcctc
     2881 ggcatcaagc cgggcaagcc actcgaggac atccgccgct tcgtgcagga ctggaagtcc
     2941 ggcgccaagc tggccggcga gctgccgccg atcactctcg cgacccttcg caagaagatg
     3001 accgcgctct cggcc
//
LOCUS       DATMCV010000042         4615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_69318, whole genome shotgun sequence.
ACCESSION   DATMCV010000042 DATMCV010000000
VERSION     DATMCV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69318"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VNU03_01095"
     CDS             complement(<1..566)
                     /locus_tag="VNU03_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73070.1"
                     /translation="MSTRREFLTTALAGGAAALAPRRVGAAPKSMTVVHESSFIKGFD
                     DFFVKTLAPEYEKLTGIKIGYETVSVGSMLTRLTTIVETKAGPEIVGTGLNWPHLFDQ
                     GLLDVTDVATEIGKKIGPWHDNIFDAVVVNKKWKAIPWGNIGQLECYRTDWFKEAGIN
                     KFPDTWEDLLSAGRLLKKKGHPFGFEIG"
     gene            886..1245
                     /locus_tag="VNU03_01100"
     CDS             886..1245
                     /locus_tag="VNU03_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6632 domain-containing protein"
                     /protein_id="HWP73071.1"
                     /translation="MLRIVLAVIGVVFIVGIYPLSLIWPSGWRWGAEHSHYLMMILGL
                     YATLGVFLLIASRNPLANRSLIWFTVWSSVVHAVIMAAQALSDPTERGHLWGDVPALL
                     VIAIVLAALTPRGRAVA"
     gene            complement(1252..1650)
                     /locus_tag="VNU03_01105"
     CDS             complement(1252..1650)
                     /locus_tag="VNU03_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP73072.1"
                     /translation="MSDATREQQAEALIAKMKAARGYMYPEWEFAARQDPEFVEAYNR
                     LYELSLGEGRHVSAKVREFVAIALLCFRGGERAGLVAHMRRAIQFGATPGELFEVLEA
                     CVVPGGAPTFHRGLGALMEVVGPAARPSAG"
     gene            complement(1752..2942)
                     /locus_tag="VNU03_01110"
     CDS             complement(1752..2942)
                     /locus_tag="VNU03_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWP73073.1"
                     /translation="MDFTYTPEQEQFRAEIRRWLAANLTDDLRIEDPTDERVAPDRET
                     FERRREWQRRMFAAGWVGISWPKEYGGRGATLMEQVIYDEECFRARAPVLPGYSGIGM
                     AGPTIIEWGTEAQKQRYLPRILGGDDIWCQGYSEPGAGSDLAGLRTRAEERGDHFVVN
                     GQKVWTSGAQFADWIYLLVRTDPSAPKHRGISYLLVDMRTPGITVRPLVLLNGHRHFN
                     EVFFVDVEVPKANLIGRQNEGWKPAMTTLMYERKGGGGRGHEEQLARLRVLATRVQLD
                     GRPAWEHPVIRQRFAQLVIEAQCQRYTRMRNLTRQLRGEPPGPEGSILKLFGSELGVR
                     IADFAGELLGPHALVNRPSEQVPDGPRWYNRVLSARQYTIAGGTSEIQRNIIGERVLG
                     LPKD"
     gene            3046..4257
                     /locus_tag="VNU03_01115"
     CDS             3046..4257
                     /locus_tag="VNU03_01115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP73074.1"
                     /translation="MAHKGMKFGIFLAPFHKVGENPTLALSRDMELIEWMDYLGYDEV
                     WIGEHHSAGWELIASPEVFIAAAAERTRHIMLGSGVTSLPYHHPLMVANRFVQLDHMT
                     RGRAMLGIGPGALVSDAYMMGIEPVTQRARMDESLDAIMQLLRCEEPVTMKTDWFELR
                     EARLHLAPYTDPHFPIAVASTITPAGVMTAGKHGIGVLSLGAGLPGGPEALSNQWKIA
                     EETAAKNGKTMDRKNWRLVVNVHVSDDDEKALQEVGRGERHETVTYFEDTLGRPPGRA
                     DDPLREGVKMGTTLVGTPDTVIRGIERLHELSAGGFGGVLFRAHEWANREQTLKSYEL
                     FARYVMPRFQGSLATVRESNEWARSNRKTIFSPNVEAVRRAFTDAGRAVPSEYRNRTV
                     GARDLDDSKPS"
     gene            4283..>4615
                     /locus_tag="VNU03_01120"
     CDS             4283..>4615
                     /locus_tag="VNU03_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73075.1"
                     /translation="MTAFVRRLIVSTGAALLAALGVVPAEARVVAVRVDRTEPFADGA
                     AFGAAGAYERVVGVARGEVDPRDPANAGIVNLDRAPRNARGLVEYETDFYLLRPADPA
                     KGNRKIVYE"
BASE COUNT          645 a         1645 c         1592 g          733 t
ORIGIN      
        1 tgcccgatct cgaacccgaa cggatgcccc ttcttcttga gcaggcggcc ggcggagagc
       61 agatcctccc aggtgtcggg gaacttgttg atgcccgctt ccttgaacca gtcggtccgg
      121 tagcactcga gctggccgat attgccccac gggatcgcct tccacttctt gttcacgacc
      181 accgcgtcga agatgttgtc gtgccacggg ccgatcttct tgccgatctc ggtcgccacg
      241 tcggtgacgt cgagcaagcc ctggtcgaag aggtgcggcc agttgagccc ggtgccgacg
      301 atctccggcc cggccttcgt ctccacgatg gtggtgagcc gggtgagcat gctgcccacg
      361 ctgaccgtct cgtagccgat cttgatcccg gtgagcttct cgtactcggg ggcgagggtc
      421 ttgacgaaga agtcgtcgaa gcccttgatg aacgagctct cgtggacgac ggtcatcgac
      481 ttcggcgcgg cgcccacgcg tcggggagcc agcgcggccg cgccgccggc cagggcggtg
      541 gtcaggaact ctcgtcgagt actcatggga cgtcctccgt tgggagcggg cgggatgagg
      601 gcgggttcat tgctcgcggg gcagaaccgg ggggacgcgc gcgacctgcg gcgcacggac
      661 aggaccgatc gactccaggc actagccgca tgcgtgcctc cgagaatgtg ccggtctggc
      721 gtgtgcggca ctctaggccc ggccgcccgt cctgtcaagg catccgggcc gcttttcggg
      781 ttgaccgggg cccggtccgg aggctagggt cgggcccgcg cgaccgcccc gaggcggccg
      841 gcgcgtggcc cgggatcggc ttccgttccg cgaggaggat cacacatgct ccgcatcgtg
      901 ctggccgtga tcggcgtcgt cttcatcgtc ggcatctacc cgctgagcct gatctggccg
      961 tcgggctgga ggtggggagc cgagcattcc cactacctca tgatgatcct cggcctgtac
     1021 gcgacgctcg gcgtgttcct gctgatcgcc tcgcgcaacc cgctggccaa ccgcagcctg
     1081 atctggttca cggtgtggtc gagcgtcgtg cacgcggtca tcatggcggc gcaggccctg
     1141 agcgatccga cggagcgcgg ccatctgtgg ggcgacgtgc cggcccttct ggtgatcgcg
     1201 atcgtgctcg ccgcgctcac cccgcgcggg cgcgcggtgg cctgagcccg gctacccggc
     1261 cgacgggcgg gcggcggggc ccaccacctc catgagcgcc ccgagcccgc ggtggaaggt
     1321 gggcgccccg cccggcacca cgcacgcctc caggacctcg aacagctcgc cgggggtcgc
     1381 cccgaactgg atcgcccgac gcatgtgggc gaccagcccg gcccgctccc cgccgcggaa
     1441 gcagagcagg gcgatggcca cgaactcgcg caccttggcc gacacgtgcc ggccctcgcc
     1501 cagcgagagc tcgtagagcc ggttgtaggc ctcgacgaac tcggggtcct ggcgcgccgc
     1561 gaactcccac tccgggtaca tgtagccccg cgccgccttc atcttggcga tcagcgcttc
     1621 ggcctgctgc tctcgcgtcg cgtcgctcaa accgtagccc cttctcgtat tcgctcggct
     1681 cgccttcggc tgcacctcgc tctgcgtctc gtattcgctc ggctcgcctt cggctgcacc
     1741 tcgccctgcg tctagtcctt tggcaagccg agcacgcgct cgccgatgat gttgcgctgg
     1801 atctcgctgg tgccgcccgc gatggtgtac tgccgcgcgc tcaggacgcg gttgtaccag
     1861 cgcggaccgt ccgggacctg ctcgctcggc cggttcacga gggcgtgggg gccgagcagc
     1921 tcccccgcga agtccgcgat gcgcaccccc agctccgagc cgaagagctt caggatggag
     1981 ccctcgggcc cgggcggctc gccgcggagc tgccgggtca ggttgcgcat gcgcgtgtag
     2041 cgctggcact gcgcctcgat cacgagctgg gcgaagcgct ggcggatgac cgggtgctcc
     2101 caggcgggcc ggccgtcgag ctgcacccgg gtggccagga cgcgcagccg ggcgagctgc
     2161 tcctcgtggc cgcggccgcc gccgcccttg cgctcgtaca tgagggtggt catcgcgggc
     2221 ttccagccct cgttctgccg gccgatcagg ttggccttcg ggacctccac gtcgacgaaa
     2281 aacacttcat tgaagtgacg gtggccgttg agcagcacga gggggcgcac cgtgatgccg
     2341 ggcgtcctca tgtcgacgag caggtagctg atgccgcggt gcttgggcgc gcttgggtcg
     2401 gtgcgcacca gaaggtagat ccagtccgcg aactgcgcgc ccgacgtcca caccttctgc
     2461 ccgttcacca cgaagtgatc gccccgctcc tccgcccgcg tgcgcaggcc ggccagatcg
     2521 gagcccgcgc ccggctcgga gtagccctgg caccagatgt cgtcgccccc caggatgcgc
     2581 ggcaggtagc gctgcttctg ggcttcggtg ccccactcga tgatggtcgg gcccgccatg
     2641 ccgatgccgg agtagccggg gagcacgggc gcgcgcgccc ggaagcactc ctcgtcgtag
     2701 atgacctgct ccatcagggt ggccccgcgt ccgccgtact ccttcggcca cgagatgccg
     2761 acccagcccg cggcgaacat ccggcgctgc cattcgcgcc gacgctcgaa cgtctcgcga
     2821 tccggcgcca cccgctcgtc ggtcgggtcc tcgatgcgca ggtcgtcggt gaggttggcg
     2881 gcgagccagc gccggatctc cgcgcggaac tgctcctgct cgggcgtgta agtgaaatcc
     2941 atgcgccact tctagcaccg gtgcgccccc gactcaaggc gtgctaggat cgccgtaacc
     3001 cggcgtcgtg tttcgccgcg gtacccctgg cccacggagg cggcattggc ccacaaaggc
     3061 atgaagttcg gcatcttcct ggcccccttc cacaaggtcg gcgagaatcc cacgctggcg
     3121 ctcagccggg acatggagct gatcgagtgg atggactacc tcggctacga cgaggtgtgg
     3181 atcggcgagc accactccgc cggctgggag ctgatcgcct cgcccgaggt cttcatcgcc
     3241 gccgccgccg agcgcacccg ccacatcatg ctcggctccg gggtcacgag cctgccgtac
     3301 caccacccgc tcatggtggc caaccgcttc gtgcagctcg accacatgac ccgcggccgc
     3361 gcgatgctcg gcatcgggcc cggcgcgctg gtgtccgacg cctacatgat gggcatcgag
     3421 ccggtgaccc agcgcgcgcg gatggacgag tccctcgacg cgatcatgca gctcctccgc
     3481 tgcgaggagc cggtcaccat gaagaccgac tggttcgagc tgcgcgaggc ccggctgcac
     3541 ctcgcgccct acaccgatcc ccacttcccg atcgcggtgg cgagcacgat cacccccgcg
     3601 ggggtgatga ccgcgggcaa gcacggcatc ggggtgctct cgctcggcgc gggcctgccc
     3661 ggcggacccg aggcgctctc caaccagtgg aagatcgcgg aggagaccgc ggccaagaac
     3721 ggcaagacga tggaccggaa gaactggcgg ctcgtggtca acgtgcacgt ctccgacgac
     3781 gacgagaagg cgctccagga agtgggccgc ggcgagcggc acgagacggt gacctacttc
     3841 gaggacacgc tcggccgccc gccgggccgc gccgacgatc cgttgcgcga aggcgtgaag
     3901 atgggcacca ccctggtcgg cactcccgac accgtcatcc gtggcatcga gcggctgcac
     3961 gagctgagcg cgggcggctt cggcggcgtt ctcttccgcg cgcacgagtg ggccaaccgc
     4021 gagcagaccc tcaagagcta cgagctgttc gcccgctacg tgatgccgcg cttccagggc
     4081 tcgctcgcca ccgtccgcga gtccaacgag tgggcgcgga gcaaccggaa gaccatcttc
     4141 agccccaacg tggaggcggt tcgccgcgcg ttcacggacg cgggccgggc ggtcccgagc
     4201 gagtaccgca accgtaccgt gggcgcccgg gatctggacg actcgaagcc gtcctgagcc
     4261 gctcttccgc ccgggtcgtg cgatgaccgc tttcgtccgg cgcctgatcg tctcgacggg
     4321 ggcggcgttg ctggccgctc tcggagtggt gcccgccgag gcgcgcgtcg tcgcggttcg
     4381 cgtggatcgc accgagccct tcgcggacgg cgccgccttc ggcgcggcgg gcgcctacga
     4441 gcgcgtggtg ggggtggcgc gcggcgaggt cgatccgcgc gacccggcca acgcgggtat
     4501 cgtcaatctc gaccgggcgc cccgcaacgc gcggggcctc gtggagtacg agaccgactt
     4561 ctacctgttg cgccccgccg atcccgcgaa gggcaatcgc aagatcgtct acgag
//
LOCUS       DATMCV010000043         2173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_534805, whole genome shotgun sequence.
ACCESSION   DATMCV010000043 DATMCV010000000
VERSION     DATMCV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534805"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VNU03_01125"
     CDS             complement(<1..246)
                     /locus_tag="VNU03_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HWP73076.1"
                     /translation="MWIDETGREQFVDFLLEAKRTTYAGQGDEATVVTPLVPGSKQLE
                     YRDGDYLYRDIYFGMAYFVGQEVVFHRELVVWSMSYAG"
     gene            complement(262..615)
                     /locus_tag="VNU03_01130"
     CDS             complement(262..615)
                     /locus_tag="VNU03_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HWP73077.1"
                     /translation="MILVAGPYRSGTNDDPTLIAKNVAHMTEVSLQLFRAGHLPVMGE
                     WFALPLIEHAGSSGIGDATFSEIFHPISRRLVARCDACLRIGGQSAGADEMVSLAKQH
                     GKTIFHSLSEVPSVR"
     gene            772..1227
                     /locus_tag="VNU03_01135"
     CDS             772..1227
                     /locus_tag="VNU03_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWP73078.1"
                     /translation="MSRSLVVLAAAAVMTSVTPGPLLAQEAGHIKVARGGVQVERAGQ
                     KTPASVGAVVQAGDVVTTGVDGSVGITFLDNSLLSAGPNSVLAIDRFAFDSTTHQGSF
                     ESSLRKGTLAVVSGKLAKQSPEAMKVKTPAAVLGVRGTEFLVRTGDPKD"
     gene            1242..1841
                     /locus_tag="VNU03_01140"
     CDS             1242..1841
                     /locus_tag="VNU03_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWP73079.1"
                     /translation="MRGLAGLAALALLLLVGCAKPARNDLYVLMPGADGKTGALTVES
                     GGKQAVLDQPYAAARVKESGQVEPGTVTEQEARQAFGAALAAQPGRPASFILYFLENR
                     DELTPDSKQTLGSALDEIARRPAPEIVVIGHTDRVGPVPYNDTLSLRRAERVRDELVK
                     VGIAADRIRVEGRGEREPLVPTPDEVAEPRNRRVEISVQ"
     gene            complement(1866..>2173)
                     /locus_tag="VNU03_01145"
     CDS             complement(1866..>2173)
                     /locus_tag="VNU03_01145"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWP73080.1"
                     /translation="CAVDGFDYTAATRQLEAGDTLCLITDGITEAARADGELYGRPRL
                     EAVLGGLGAAATAADIGEAIRRDVAAFTGDAEASDDMAILILRFTGPNPLPSGERAG"
BASE COUNT          346 a          739 c          751 g          337 t
ORIGIN      
        1 tccggcatag ctcatcgacc acaccaccag ttcccgatgg aagacgacct cctggccgac
       61 gaagtaagcc atcccaaagt agatgtcgcg gtagagatag tcgccatcgc ggtactcgag
      121 ttgctttgat cccgggacca gtggcgtcac caccgtcgcc tcgtcaccct gcccggcgta
      181 cgtcgtacgc tttgcctcga gcaagaagtc cacgaactgc tctcggccgg tctcgtcgat
      241 ccacatgcgt gacgccacat cctacctcac gcttggcact tcactgagcg agtggaagat
      301 cgtcttgccg tgctgcttgg ccaagctgac catctcgtcg gcaccagccg actgaccacc
      361 aatgcgaaga caggcgtcgc atctggcaac cagccggcgg ctgatgggat gaaagatctc
      421 gctgaaggta gcatcgccga tgcctgacga accggcatgc tcaatgagcg gcagggcgaa
      481 ccactcaccc atgactggaa ggtgcccggc tcggaagagc tgaagggaca cctcggtcat
      541 gtgggcaacg ttcttggcga tgagcgtcgg atcgtcattg gtgcctgagc gatagggccc
      601 tgcaacgaga atcatgagtg ggcgcaaggt gagacggtcc tcccttgtga tgcctaacta
      661 gagtgcgggg ttgttcggac ttgccggccg cgagccttgg ctttcggcta ttataggccc
      721 cgctcggccg gtcgaggccg gagcgatcgt ggagaactgg aggaccatcg catgtctcgc
      781 tcgctcgtcg tgctggccgc cgcggccgtg atgacctccg tgacgccggg tcccctcctc
      841 gcccaggagg ccgggcacat caaggtggcg cgcggcgggg tgcaggtcga gcgcgcgggg
      901 cagaagacgc cggcctcggt cggcgcggtc gtgcaggcag gcgacgtggt gaccaccggg
      961 gtggacggct cggtcggcat caccttcctc gacaacagcc tgctctccgc cgggcccaac
     1021 agcgtgctcg ccatcgaccg gttcgcgttc gactcgacca cgcaccaggg cagcttcgag
     1081 agctccctcc gcaagggcac cctcgcggtc gtctcgggca agctcgccaa gcagtcgccg
     1141 gaggccatga aggtcaagac ccccgccgcg gtgctgggcg tgcgcggcac cgagttcctc
     1201 gtccgcaccg gggacccgaa ggactgacgc ccccgcctgc gatgcgtggc ctcgcggggc
     1261 tcgcggccct cgcgctcctg ctcctggtcg gctgcgccaa gccggcccgc aacgatctct
     1321 acgtgctcat gccgggggcg gacggcaaga ccggcgcgct caccgtggag agcggcggca
     1381 agcaggcggt gctcgaccag ccctatgcgg cggcgcgggt gaaggagtcc gggcaggtgg
     1441 agccagggac ggtgaccgag caggaggcgc gtcaggcctt cggcgcggcg ctggccgcgc
     1501 agcccggccg tccggcctcc ttcatcctct atttcctcga gaatcgcgac gagctcacgc
     1561 cggattccaa gcaaacgctg gggagcgcgc tcgacgagat cgcgcggcgc ccagccccgg
     1621 agatcgtggt catcggccac accgaccggg tcgggcctgt gccctacaac gacacgctgt
     1681 cgctccgacg cgcggagcgg gtgcgcgacg agctcgtgaa ggtcggcatc gccgccgatc
     1741 gcattcgcgt cgaggggcgc ggggagcggg aacctctggt gcccacgcct gacgaggtcg
     1801 cggagccgcg gaatcggcgg gtggagatca gcgtccagta ggtgcgggct atccgtgacc
     1861 acccctcacc ctgccctctc ccctgagggg agagggttcg ggccggtgaa acggaggatc
     1921 aggatggcca tgtcgtccga ggcctcggcg tcgccggtga aggcggccac gtcgcgccgg
     1981 atggcctcgc cgatgtcggc ggcggtcgcg gccgctccca ggccgcccag caccgcttcg
     2041 agtcgcggcc gcccgtagag ctcgccgtcc gcgcgggccg cctcggtgat cccgtcggtg
     2101 atcaggcaca gcgtgtcgcc cgcctcgagc tggcgcgtcg ccgcggtgta gtcgaagccg
     2161 tcgaccgcgc aga
//
LOCUS       DATMCV010000044         4752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_334575, whole genome shotgun sequence.
ACCESSION   DATMCV010000044 DATMCV010000000
VERSION     DATMCV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334575"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VNU03_01150"
     CDS             <1..165
                     /locus_tag="VNU03_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HWP73081.1"
                     /translation="VFCGEENDVLVDPSGGRHQTFTEDCTVCCRPNLITLTFDDDGDV
                     TIEVVQEYEA"
     gene            complement(170..973)
                     /locus_tag="VNU03_01155"
     CDS             complement(170..973)
                     /locus_tag="VNU03_01155"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWP73082.1"
                     /translation="MRFDGKVALISGAGSGIGRATALGFAGRGAKVAVADLDGGRADA
                     VTAEIRAAGGTAIAITADAGAPDGIDAMVGGAVRAFGGLDILHNNAFGNPPLPPGKSR
                     LAFTGDLDDAIWTHIVDLALTGVFRATKRAIPELLARGGGAIVNTASISGLFADFAIG
                     AYNAAKAGVINLTRVTAIEYAGRGIRVNCVCPGAIDTPLLRPSLGIPGFADTTTAMIP
                     MRRLGRPEEIASVVLFLASDLASYVTGAAVVADGGLTAQTGMPSRIPLP"
     gene            complement(987..2222)
                     /locus_tag="VNU03_01160"
     CDS             complement(987..2222)
                     /locus_tag="VNU03_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73083.1"
                     /translation="MTAIGFEIPADITRIVAGLTQFVRAEVVTRHEKHAALLDDPRQR
                     YGPDGRYAPAVVDLIREVRVASAAAGYFNLCAPISLGGLGQGHLAYYVAWEAINHLCG
                     PHHWLGTFALSHWAFGPSVVLEQLTAEARERALAGLVSGRQSMCFGLSEPGAGSDATM
                     ITTRALAEGDGWRISGRKMWTSNITFADWIVVFAVTDPARAAAKRGGISAFLVPTTAP
                     GFALQRVTLLQGDIGGAEGESTFDAVLVEPWQLVGPLHEGFRIALLGVSLGRLYNAAR
                     AVGLARWALEKAVAYAGQRVAFGVPIAEHQGVAFPLAESAMEVHAAHLAGLNAALLLD
                     RGERAIKELSIAKAYSVEVCLRAVDRAIQTHGGMGLTNEVGLVHAYNTLRIINIADGT
                     NEILRRTIFQQLARGDLDL"
     gene            2292..4313
                     /locus_tag="VNU03_01165"
     CDS             2292..4313
                     /locus_tag="VNU03_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP73084.1"
                     /translation="MALRIGIDIGGTFTDLVALDEATGSVVDTKALSTPQDLLQGILR
                     CVDQAGARLDDCRLAIHGTTIGINALLEHKGARTGLVTTEGFRDVLEIGRGNFLRMYD
                     VLYRRPTPLVPRGRRLEVGERVTARGEILAPLDEAAVRAAARQLAADGVESVAVSFLF
                     SFRNPEHERRAAEILAEMLPGVSISVSHRITQEWREYERTSTTVVNAYVRPIMDRYLG
                     AFERGLAGRGFRGQLLITQSNGGAFSVQAAASKPVHTIESGPAAGAIGCASLAQALGA
                     DRLISFDMGGTTAKCAIVAGGLVQTTDEYHVDGHPLRIPVIDITEVSAGGGTIAWIDA
                     GGALALGPQSAGADPGPVCYGRGGAEPTVTDANLVLGRIAAGRFLGGTMPLDQPAAAR
                     AIEERLGLPLGLARDSAAAGVVRLADVKMALAVRSITTERGLDPRDYTLVAYGGGGPL
                     HAVAIARELGIPRVIIPPAPSTFSAWGMLATDLRHDLVRTVLEPLERTDGGWAEARYR
                     EMETEIEAILPPVGTAVLHRAADLRYLGQEHTVTVEIGPLADWASLRGRFDAAHQRAY
                     GYAAPDVEVQLLNLRLAVVYPLERAPLPALPRGSGRAPAFETRKIYSTLLRDLLEYRV
                     YGRDRLRAGDVVAGPAAIEEAGTTTMLEPGDRLQVEGNACLVIDVAKGV"
     gene            4316..>4752
                     /locus_tag="VNU03_01170"
     CDS             4316..>4752
                     /locus_tag="VNU03_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP73085.1"
                     /translation="MGNQPLDAVTIEVVRAALNAAAERMRITMIKTAYNHIISESLDF
                     GCAIFDPDVQMIAQGIGLPVFQGHLGFPIQATLADRGLEAFREGDVFMHNDPYAGNGN
                     HLNDVAMYAPVFWEGALTGFVSVKAHWSDVGGPVPSSMQIGSR"
BASE COUNT          663 a         1712 c         1752 g          625 t
ORIGIN      
        1 gtcttctgcg gcgaggagaa cgacgtgctg gtcgacccga gcggcggccg ccaccagacg
       61 ttcaccgagg actgcacggt gtgctgccgc cccaacctga tcacgctgac gttcgacgac
      121 gacggcgacg tgacgatcga ggtggtgcag gagtacgagg cctgacccat cagggcaggg
      181 ggatccgcga cggcatcccg gtctgcgcgg tgaggccccc gtcggccacc accgccgcgc
      241 cggtgacgta ggacgcgagg tcggaggcga ggaacagcac cacgctcgcg atctcctccg
      301 gccgtccgag gcgccgcatc gggatcatcg cggtggtggt gtccgcgaag ccggggatgc
      361 cgagcgacgg gcgcagcagc ggggtgtcga tcgcccccgg gcagacgcag ttgacccgga
      421 tgccgcgccc cgcgtactcg atcgcggtca cccgcgtgag attgatcacc ccggccttgg
      481 ccgcgttgta ggcgccgatc gcgaagtcgg cgaagaggcc ggagatcgac gcggtgttga
      541 cgatggcgcc tccgccgcgc gcgagcagct ccgggatggc ccgcttggtc gcgcggaaga
      601 ccccggtgag ggccaggtcg acgatgtggg tccagatcgc gtcgtcgaga tcgccggtga
      661 aggcgaggcg gctcttgccc ggaggcagcg gcggattgcc gaacgcgttg ttgtgcagga
      721 tgtcgagccc gccgaaggcc cgtaccgcgc cgcccaccat cgcgtcgatc ccgtccgggg
      781 cgcccgcgtc ggcggtgatc gcgatcgcgg tgccgcccgc ggcgcggatc tccgcggtca
      841 ccgcgtccgc ccgcccgccg tcgaggtccg ccaccgccac cttcgcgccg cgtcccgcga
      901 agccgagcgc ggtcgcccgg ccgatgcccg agcccgcgcc cgagatcagc gccaccttgc
      961 cgtcgaatcg catgcaggtc tccgcctcag aggtcgagat cgccgcgggc gagctgctgg
     1021 aagatggtgc gccgcaggat ctcgttggtg ccgtcggcga tgttgatgat gcggagcgtg
     1081 ttgtaggcgt gcacgagacc cacctcgttg gtgaggccca tgccaccgtg ggtctggatg
     1141 gcccgatcca ccgcgcgcag gcacacctcg accgagtagg ccttggcgat cgacagctcc
     1201 ttgatcgcgc gctcgccgcg gtcgaggagc agcgccgcgt tgaggccggc caggtgggcc
     1261 gcgtgcacct ccatggcgga ctcggcgagg gggaacgcga cgccctggtg ctcggcgatc
     1321 ggcaccccga aggccacccg ctggcccgcg taggccaccg ccttctcgag cgcccagcgg
     1381 gcgaggccga ccgcgcgggc cgcgttgtag aggcgtccga gcgagacgcc gaggagcgcg
     1441 atccggaaac cctcgtggag cgggccgacc agctgccagg gctcgaccag caccgcgtcg
     1501 aaggtggact cgccctcggc cccgccgatg tcgccctgga gcagggtcac ccgctgcagc
     1561 gcgaagccgg gggcggtcgt gggcaccagg aacgcgctga tgccgccgcg cttggccgcc
     1621 gcgcgggcgg gatcggtcac cgcgaagacc acgatccagt ccgcgaacgt gatgttcgac
     1681 gtccacatct tccggccgga gatgcgccag ccgtcgccct cggccagcgc gcgggtggtg
     1741 atcatggtgg cgtcggagcc ggcccccggc tccgagagcc cgaagcacat ggattggcgg
     1801 cccgagacga ggcccgccag cgcgcgctcg cgggcctccg cggtgagctg ctcgagcacc
     1861 acgctcgggc cgaaggccca gtggctgagc gcgaaggtcc cgagccagtg atgcggcccg
     1921 cagaggtggt tgatcgcctc ccaggccacg tagtaggcga gatggccctg gccgagcccg
     1981 ccgagcgaga ttggcgcgca gaggttgaag taccccgcgg cggcggaggc gacgcggacc
     2041 tcccggatca ggtcgaccac cgcgggcgcg tagcggccgt cgggcccgta gcgctggcgc
     2101 gggtcgtcga gcagggccgc gtgcttctcg tggcgcgtga ccacctcggc ccggacgaac
     2161 tgcgtgaggc ccgccacgat gcgggtgatg tccgccggga tctcgaagcc gatcgcggtc
     2221 acgcggggag ggtatcacga gcgggcgatc cgcttgcgac gcggggcggg gcaccctata
     2281 ctccgcgaaa cgtggctctc cggatcggca tcgacatcgg cggcaccttc acggacctgg
     2341 tcgcgctgga cgaggcgacc ggttcggtcg tggataccaa ggcgctctcg accccccagg
     2401 acctgctgca gggcatcctc cgctgcgtgg accaggccgg cgcccggctc gacgactgcc
     2461 ggctcgccat ccacggcacc acgatcggga tcaacgccct gctggagcac aagggggccc
     2521 ggaccggcct cgtcaccacc gagggcttcc gcgacgtgct cgagatcggg cgcggcaact
     2581 tcctgcgcat gtacgacgtg ctctaccggc ggccgactcc gctggtgccg cgcggccggc
     2641 ggctcgaggt gggcgagcgg gtgactgccc gcggggagat cctggccccg ctcgacgagg
     2701 ccgcggtgcg ggcggcggcc cggcagctcg ccgccgacgg cgtggagtcg gtcgcggtgt
     2761 cgttcctgtt ctcgttccgc aacccggagc acgagcgccg cgcggccgag atcctggccg
     2821 agatgctgcc cggcgtctcg atctcggtct cgcaccgcat cacccaggag tggcgcgagt
     2881 acgagcgcac cagcaccacc gtggtcaacg catacgtgcg gccgatcatg gatcgctacc
     2941 tgggcgcctt cgagcgcggg ctcgccggcc gcggcttccg cggtcagctc ctcatcaccc
     3001 agtccaacgg cggggccttt tcggttcaag cggcggcctc caagccggtg cacacgatcg
     3061 agtcgggccc ggccgcgggg gccatcggct gcgcgagcct ggcccaggcc ctcggggccg
     3121 accggctgat ctccttcgac atgggcggca ccaccgccaa gtgcgcgatc gtggcgggcg
     3181 ggctcgtgca gaccaccgac gagtaccacg tggacggcca cccgctccgc atcccggtga
     3241 tcgacatcac cgaggtgagc gcgggcggcg gcaccatcgc gtggatcgac gcgggcgggg
     3301 cgctcgcgct ggggccgcag agcgcggggg ccgacccggg cccggtctgc tacgggcgcg
     3361 gcggcgcgga gccgaccgtc accgacgcca acctggtgct ggggcgcatc gcggccggcc
     3421 gcttcctcgg cggcacgatg ccgctcgacc agcccgcggc ggcccgcgcg atcgaggagc
     3481 ggctcggcct gccgctcggc ctcgcgcgcg acagcgcggc ggcgggggtc gtccggctcg
     3541 ccgacgtgaa gatggcgctc gcggtgcgca gcatcaccac cgagcgcggg ctcgacccgc
     3601 gcgactacac cctggtggcc tacgggggcg gcggcccgct gcacgcggtc gcgatcgcgc
     3661 gggagctggg catcccgcgc gtgatcatcc cgcccgcgcc ctcgaccttc tcggcgtggg
     3721 gcatgctcgc gaccgatctt cgtcacgacc tggtgcgcac cgtgctcgag ccgctcgagc
     3781 ggaccgacgg cggctgggcg gaggcgcgct accgggagat ggagaccgag atcgaggcca
     3841 tcctgccgcc ggtgggcacc gcggtcctcc accgggcggc ggatctgcgc tacctcgggc
     3901 aggagcacac cgtgaccgtc gagatcggcc ccctcgcaga ctgggcgtcg ctgcggggcc
     3961 ggttcgacgc ggcccaccag cgcgcctacg gctacgccgc gccggacgtg gaggtacagc
     4021 tgctgaacct gcgcctcgcg gtggtctacc cgctcgagcg cgccccgctg ccggcgcttc
     4081 cgcgcgggag cggccgcgcc cccgccttcg agacccgcaa gatctactcc acgctgctcc
     4141 gcgacctgct cgagtaccgc gtgtacgggc gcgaccggct gcgggccggc gacgtggtcg
     4201 cggggccggc cgcgatcgag gaagcgggca ccaccaccat gctggagccg ggcgatcgcc
     4261 tccaggtcga aggcaacgcc tgcctggtga tcgacgtggc gaaaggggtc tgaccatggg
     4321 gaaccagccg ctcgacgcgg tgaccatcga ggtggtccgc gccgcgctca acgccgccgc
     4381 cgagcgcatg cggatcacca tgatcaagac cgcctacaac cacatcatca gcgagagcct
     4441 cgacttcggg tgcgccatct tcgaccccga cgtgcagatg atcgcccagg gcatcgggct
     4501 gccggtgttc cagggtcacc tgggcttccc gatccaggcg accctcgccg accgcgggct
     4561 cgaggccttc cgcgaaggcg acgtgttcat gcacaacgac ccctacgcgg gcaacggcaa
     4621 ccacctgaac gacgtggcga tgtacgcccc ggtgttctgg gagggcgcgc tcaccggctt
     4681 cgtctcggtg aaggcccact ggagcgacgt gggcggtccg gtgccgagct cgatgcagat
     4741 cggctcgcgc ga
//
LOCUS       DATMCV010000045         2712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_201842, whole genome shotgun sequence.
ACCESSION   DATMCV010000045 DATMCV010000000
VERSION     DATMCV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201842"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1960
                     /locus_tag="VNU03_01175"
     CDS             203..1960
                     /locus_tag="VNU03_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWP73086.1"
                     /translation="MVTAGCYARKSTDEGDKAADAKSCARQIARAKEYAATRGWRFDE
                     RYVFSDDGVSGAEFKNRPSLNALLSAVKETHRLNMLIVSEQSRLGRDTIRTLALIQAL
                     NDSGVKMWSYLEDKELSLDDELGEIESFMKSWAGASERRKASQRVRDKMRQLAEQGRS
                     TGGRLFGYETKDGARTVQPSEAAVIRRIFTRRSEGAGYFKIARELERDQVASPRGSRL
                     WSPTQVGSILANETYAGVSVWGRTRQTKRRGTSVTEQSPDAVVRREAPALRIVSETLW
                     REVRAVNQAATESSWRTPDGRRKSRPTTGRHFLPQFIACGVCGGSMHVRFDKKRREFL
                     FCTNRHLLGKEKCPNARRLPVAFAERAFMHAFEEALAGALVMEKLEEVLEYQRRAQRD
                     PAPLQAEAKRLRAEISRLVASLARGDVEEVHQAVAERKARLAQVEDELAGTAAIEGID
                     VERFRASIEEVAEDWRAHLRRNKAVAAQVLRKILPERVAVTPLAEGGWSFEGATNYST
                     ILEEVGYKAVLDALRSHGMLSGTAELPHEVKHPAGECRPEYACWPGETKLSKTPCPSP
                     CSARPSARGTTRSRRTPPR"
     gene            complement(1881..2385)
                     /locus_tag="VNU03_01180"
                     /pseudo
     CDS             complement(1881..2385)
                     /locus_tag="VNU03_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144277.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
BASE COUNT          460 a          884 c          973 g          395 t
ORIGIN      
        1 gcctcgcggc gccgagcgga cttgaaccac caggtaggct ccgctcgtga tgtgcgtcag
       61 cggcggaaaa cacagacaag atccggcaga ggcccgttac agcacctcct tacgtgggcg
      121 tcgcgtagaa ggcaggaacc ccggtccatc gcggccagca acaccccgtc gtttccgctg
      181 cggttcatct acactagggg ctatggtgac cgccgggtgc tatgcgagga agtcgaccga
      241 cgagggcgac aaggcggccg atgcgaagag ctgtgcgcga cagatcgcgc gcgccaagga
      301 gtacgcggcc acgcgcggct ggcggttcga tgagcggtat gtgttttcag acgacggggt
      361 gagtggagcg gagttcaaga acagaccgtc gctcaacgcg ctgctatccg cggtcaagga
      421 gacgcaccgg ctcaacatgc tcatcgtctc ggagcagagc cggctcggcc gcgacaccat
      481 ccgcacgctg gcgctgatcc aggcgctcaa cgacagcggc gtgaagatgt ggtcgtacct
      541 ggaggacaag gaactctcgc tcgacgacga gctcggcgag atcgagtcct tcatgaagag
      601 ctgggcgggg gcgtccgagc gccggaaggc gagccagcgc gtgcgggaca agatgcgaca
      661 gctcgccgag cagggacgca gcaccggcgg ccgactgttc ggctacgaga cgaaggacgg
      721 tgcgcgcacg gtccagccga gtgaggccgc ggtcatccgc cggatcttca cgcggcgcag
      781 tgagggcgcg ggctacttca agatcgccag ggagctggaa cgggaccagg tcgcctcgcc
      841 gcgcggctcc cggttgtggt cgcccaccca ggtcggctcg atcctcgcca acgagacgta
      901 cgcgggcgtc agcgtgtggg gcaggacccg gcagaccaag cgccgcggca cgtcggtgac
      961 ggagcagtca ccggacgcgg tggtgcggcg cgaggcgccc gcgctccgca tcgtctccga
     1021 gacactgtgg cgcgaggtgc gggcggtgaa ccaggcggcg accgagtcga gctggcgcac
     1081 gcccgacggg cggcgcaagt cgcggccgac cacgggccgc cacttcctgc cgcagttcat
     1141 cgcgtgcggc gtctgcggcg gcagcatgca cgtccgcttc gacaagaagc gccgggagtt
     1201 cctgttctgc accaaccgtc acctcctcgg gaaggagaag tgccccaacg cgcggcgcct
     1261 gccggtggcg ttcgcggagc gggcgttcat gcacgcgttc gaggaggcgc tggctggtgc
     1321 gctcgtcatg gagaagctgg aggaagtcct ggagtaccag cgccgcgccc agcgagaccc
     1381 cgcgccgctt caggccgagg ccaagcgcct ccgcgcggag atcagccgcc tggtcgcctc
     1441 gctggcccgg ggtgacgtcg aggaggttca ccaggccgtc gcagagcgca aggccaggct
     1501 ggctcaagtg gaggacgagc tggctgggac ggccgcgatc gagggcatcg acgtcgagcg
     1561 attccgagcg agcatcgagg aggtggcgga ggactggcgg gcccacctga ggaggaacaa
     1621 ggcggtggcc gcgcaggtgc tcaggaagat tctgcccgag cgggtggcgg tgaccccgct
     1681 ggccgagggc ggctggtcct tcgagggcgc gaccaactac agcacgatac tggaggaggt
     1741 gggctacaag gcggtcctgg acgccctgag gtcccacggg atgctctccg gcaccgcgga
     1801 gctgccgcac gaggtcaagc acccggccgg cgagtgccgc ccggaatacg cctgctggcc
     1861 gggggagacc aaactatcta agacgccgtg cccatcacca tgcagtgccc ggccgagcgc
     1921 gagaggcacg actcgatctc gcagaactcc tcctcgctga gccgccccgc gcgtcgctcg
     1981 gcccagaagc gccagttgtc ggtgccggag ccgatgtcct cggtgcgcca gcggccgcgc
     2041 agcatcggcc cgccggtcac catgatcgcg ggcacgtcgg cggaggccgc gcccatcagc
     2101 atggcgggtg tggtcttgtc gcagcctgag agcagcacca ctcccgccat gctgatgggc
     2161 gcggatgcac tcctccacgt ccatcgccat caggttgcgg aagagcatgg tggtgggctt
     2221 catgaggatc tcacccagcg agatggtcgg aaactcgagg gggaagccgc ctgccgagag
     2281 caccccgcgc ttcaccgcct ccgccacctg ccgcaggtgg gcgttgcagt tggtcagctc
     2341 ggaccaggag ttggcgatgc cgatcacggg ccggccgtcg aacatcagct cgctgaagcc
     2401 ctccgccttg atccacgagc ggtgggagaa gccgtccagg tccttcttgc cgaaccagtt
     2461 gtggctgcgc agggtgggcg tgctcaccgg agacctccct cagtgcgggt gggcgaattc
     2521 gtgttcgcta gtatccgtct ttcgccacgc cttgagggat ttatcagcta tgcctccgac
     2581 gcctgcggaa ctgcttcgac ttccgctgct ttccaatcgt ggggctgcga accgctgggt
     2641 gacggactcc ttcgccatcc gctgctctct gtggcgcctc gcggcgccga gcggacgtga
     2701 accaccaggc ag
//
LOCUS       DATMCV010000046         7481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_201906, whole genome shotgun sequence.
ACCESSION   DATMCV010000046 DATMCV010000000
VERSION     DATMCV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201906"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VNU03_01185"
     CDS             complement(<1..1268)
                     /locus_tag="VNU03_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73087.1"
                     /translation="MKLFSWLVLSLLLLAGPVAAQPTQLTIVQPAEATTMDPGRSTQV
                     LTVNYFYNLYDSLTRWDAALQLQPGLATSWKAVNDTTWEFTLRPGVKFHDGSPLTAED
                     VRATIERNLVPGKTVVTAGFTTIESVQAASPTLVRVVTRKPDPLLPVRMAQMGSQILP
                     ARLTTDEGAKELARRPIGSGAYRFVEWVKDERLVMEANRDWWGWEGRAPAVDRVLWKA
                     IPDDFPRLVALEKGDADIVTSVPPDRIRSVAEGRNTRVVSVPSTRTVEFNINSTQPPL
                     SDKRVRQALHYALDVPAIIRSLFAGLGRPFAGGLADTDFGYNPALKPYPYDPARARAL
                     LAEAGSPGGIDVTLHAGMGTMVNDKQLLEVIADMWAKVGIRARVAMMEMGARQRMVNE
                     RALPPGGLLLINVQSTLLDADGSLWRILHPT"
     gene            1442..2734
                     /locus_tag="VNU03_01190"
     CDS             1442..2734
                     /locus_tag="VNU03_01190"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HWP73088.1"
                     /translation="MKGVTLPTETVFVMENSPIKFGLGAMDEIGFDATRLGIKKALIF
                     TDRHVAELGLSERVRALLEEQGVKADVWDGVEVEPTDRSMEEAGEYARSKEFDGLVAV
                     GGGSTIDTCKAANLLTCYPAPLLDYINKPVGRGVPVPGPMRPLIAVPTTAGTGSETTA
                     VAVTHVVDQNVKAGVSHRLLRPTLGVVDPLNTLSAPPEVTAAAGADILTHAIESYTTR
                     PYDARAKHHPPDRPAYIGANPASDIWCEKAMEYAARYLRRAVLNGLDVEARLHLALAA
                     NYAGIGFGNAGVHIPHAVAYPIAGLVRDYVPSGYRTRHPMVPHGMSVILTAPAAFRFT
                     YATSPERHLRAAELLGVPVADLSEAERREALPRALLSIMRDIGIPNGLTAIGYTERDV
                     PALIEGTLRQPRLLSGAPRPVGQPELELILRDAMQYWG"
     gene            complement(2737..4113)
                     /locus_tag="VNU03_01195"
     CDS             complement(2737..4113)
                     /locus_tag="VNU03_01195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP73089.1"
                     /translation="MSAPTAEVVICGAGIAGVAAAYHLAVRRGIRSVVLIDERPPLSL
                     TSDKSAESYRNWWPGPGDDMAALMNRSIDLMEELARESDNVFLMNRRGYLFATAQPGQ
                     IEGYVKAGQDAAARGVGPLRLHRSPGGDYQPSPATGYEGQPTGTDVVTDRSLIRRHFP
                     YLAEEAVALLHARRCGWFSGQQLGMYMLERAREAGVRLTEGRVDAVETAGGRVTGVRV
                     SGAGGGRTIGTPRFVNAAGPYVKAVGRLLDVEIPVFCERHAKVAFHDTQGVVPRHAPM
                     MIWTDPVRLPWSDDEREELGQAEGLRHLLEEMPAGVHGRPEGGGDSPVMLGVWTYDVE
                     PMEPRFPVEFDPAYAEIVIRGMTRLIPGLSAYVPRLPKHFVDGGYYTKTEENRLLSGP
                     LPVEGAYVLGGLSGYGMMSSNGAADLLADYIAGRPLPRYAPAFHLDRYQDPAYRTLLA
                     EWGDSGQL"
     gene            4287..4595
                     /locus_tag="VNU03_01200"
     CDS             4287..4595
                     /locus_tag="VNU03_01200"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWP73090.1"
                     /translation="MMTRQSDRRRHPRSDVSWLVVVEAGKRRFLLQTMDISARGTKVR
                     PRERLDIGTPAQLRFRPPDGTPFNVPALVWRTDSDGLAFLFMGDIQDRLRRYGRLLAS
                     "
     gene            complement(4621..6933)
                     /locus_tag="VNU03_01205"
     CDS             complement(4621..6933)
                     /locus_tag="VNU03_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWP73091.1"
                     /translation="MAALWEGRFRVLRRLTLFAAALLVILIVVGGGLAIYSLRALSKF
                     ERVEARRSTLLYAAPPVLRSGVSIGALDLAGILGRLGYRESRTAAGPGQFSRSDSAWD
                     IDAGSGAGGAGRVSLTVSGGRITRLRQNGTEVQSVALPPELLASAGAGMGESIRPVRL
                     TDVPPVLRTAVLAIEDERFYEHGGLDPRGVLRALWANVRKGRVVEGGSTITQQLVKSR
                     LLTPARTYSRKLNEALLSTALEWRYSKDQILEAYLNEIYLGQTGGSAVRGVGAASRAY
                     FGKEVHQLTLPEAALLAGMIRGPNNYSPVANPDRARERRDVVLTRLRDLGKISEADYR
                     RARREPVKARITPGSGLVAPYFVDYVRADLERSTEMDLADQHGVRVYTTLDPVLQRLA
                     EAAVVKGIDRLETSRPRLRRPSPEERLQAALIVLDTSTGQVRALVGGRDYRLSQFNRA
                     VLARRQPGSAFKPFVFLAALTPRPDGPRFTAASLIEDAPITIVVDGKPWSPKNYDDRY
                     QGQVTVRRALEGSLNTATVRLAQAVGLNTVVDTARSLGVEGDLKPVPALALGVFETTP
                     LDLARAYLPLANGGIAPSGGVVDTVTDDSGRAIWSASRETRPVIGAPEAFLVTSLLEG
                     VINSGTGASARTLGVPGAVAGKTGTTNDGRDAWFVGYSSNLLALVWVGFDDGAPAGLS
                     GAEGALPIWSEFMRQALDIYPGGAFPEPPGVTYAKIDATNGRKATAYCPVVVTEVFLT
                     GTEPPPCEEHGGVSEQIGRWWDKVWDWFKK"
     gene            complement(6974..7360)
                     /locus_tag="VNU03_01210"
     CDS             complement(6974..7360)
                     /locus_tag="VNU03_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73092.1"
                     /translation="MRAFAKFGSALAALLMSLIALTGTPAPAPAGERHAGTVLAVDPQ
                     ARTLTVDEFGVGGERRALRFQVPRDALVVWSQRNQAGRDLKDAFRDSTITLGDVRIGD
                     FVVVELSDDPEVARLVMVTLRRGAGS"
BASE COUNT         1077 a         2761 c         2596 g         1047 t
ORIGIN      
        1 gtcgggtgca ggatgcgcca gaggcttccg tcggcgtcga gcagcgtcga ctgcacgttg
       61 atcaggagca ggcccccggg cggcagcgcc cgctcgttga ccatgcgctg ccgcgctccc
      121 atctccatca tggccacgcg ggcgcggatg cccaccttcg cccacatgtc ggcgatcacc
      181 tcgagcagct gcttgtcgtt gaccatggtg cccatccccg cgtggagcgt cacgtcgatc
      241 ccgccggggc tccccgcctc ggccaggagc gcccgcgccc gcgcgggatc gtacggatac
      301 ggcttgagcg cggggttgta gccgaagtcg gtatccgcga gcccgcccgc gaacggccgg
      361 ccgagtcccg cgaagaggct ccggatgatc gcgggcacgt cgagcgcgta gtggagcgcc
      421 tgacgcaccc gcttgtcgga cagcggcggc tgcgtcgagt tgatgttgaa ctcgacggtg
      481 cgggtggacg gaacgctcac cacccgggtg ttccggccct ccgcgaccga cctgatccgg
      541 tcgggcggga cgctggtcac gatgtccgcg tcgcccttct cgagcgcgac caggcgcgga
      601 aaatcatccg ggatcgcctt ccacagcacg cggtcgaccg cgggggcgcg gccctcccag
      661 ccccaccagt cgcggttggc ctccatgacg agccgctcgt ccttcaccca ttccacgaaa
      721 cgatacgccc cgctgccgat cggccgacgc gccagctcct tcgcgccctc gtcggtggtg
      781 aggcgggcgg gcaggatctg cgagcccatc tgcgccatgc gcacgggcag gagcggatcc
      841 ggcttccggg tgacgacccg gaccagcgtg ggactcgccg cctgcaccga ctcgatcgtg
      901 gtgaagccgg cggtcaccac cgtcttcccc gggaccaggt tccgctcgat cgtggcccgc
      961 acgtcctccg cggtgagcgg cgagccgtcg tggaacttca cacccggtcg cagggtgaac
     1021 tcccacgtgg tgtcgttgac cgccttccac gaggtggcca ggcccggctg cagctgcagc
     1081 gcggcgtccc accgggtgag ggagtcgtag aggttgtaga agtagttgac ggtgagcacc
     1141 tgggtgctgc gaccggggtc catcgtggtc gcctcggccg gctggacgat ggtgagctgg
     1201 gtgggctggg cggccaccgg accagcgagc aggagcagcg acagcaccag ccaggagaag
     1261 agcttcattg gcagccgaag agggtcatcg gtcttccctc ccttggggcg ggccgaacat
     1321 agcgcagccc gccgctccgg ggcaacacgc aacccgcgcc gacgtcatgc cgacgctata
     1381 atggccgcca cgccggccgc ggggccggcc gcccgatccc tcgtcaccca ctggaggccg
     1441 tatgaagggc gtgaccctgc cgaccgagac cgtcttcgtg atggagaact cgccgatcaa
     1501 gttcgggctg ggggccatgg acgagatcgg cttcgacgcc actcgcctcg gcatcaagaa
     1561 ggccctcatc ttcaccgatc gccacgtggc ggagctgggc ctgtccgagc gggtgcgcgc
     1621 cctgctggag gagcagggcg tgaaggccga cgtgtgggac ggggtcgagg tggagcccac
     1681 cgaccggtcg atggaggagg cgggcgagta cgcgcgctcc aaggagttcg acggcctcgt
     1741 ggcggtcggc ggcgggagca ccatcgatac gtgcaaggcg gcgaacctcc tcacctgcta
     1801 cccggctccg ctgctcgact acatcaacaa gccggtgggg cggggcgtac cggtacccgg
     1861 cccgatgcgc ccgctgatcg cggtgccgac caccgcggga accggcagcg agaccaccgc
     1921 ggtggcggtc acgcacgtcg tcgatcagaa cgtcaaggcg ggagtctctc atcggctcct
     1981 gcgccccacg ctcggagtgg tcgatccgct caacaccctg agcgcgccgc ccgaggtgac
     2041 cgcggcggcg ggcgccgaca tcctcaccca cgccatcgag tcgtacacca cgcgacccta
     2101 cgacgcccgc gccaagcatc acccgcccga tcggcccgcc tacatcggcg cgaacccggc
     2161 gagcgacatc tggtgcgaga aggcgatgga gtacgcggcg cgctacctcc gccgcgcggt
     2221 gctgaacggc ctcgacgtgg aggcgcgcct gcacctggcc ctggccgcca actacgccgg
     2281 catcggcttc gggaacgcgg gggtgcacat cccccacgcg gtggcctatc cgatcgcggg
     2341 cctcgtgcgc gactacgtcc cgagcggcta ccgcacccgt cacccgatgg tgccgcacgg
     2401 catgtcggtg atcctcaccg cccccgccgc gttccgcttc acctacgcga cgtcgccgga
     2461 gcgccacctt cgcgccgccg agctgctggg ggtcccggtc gccgacctga gcgaggcgga
     2521 gcggcgcgag gcgctgccgc gggcgctgct ctcgatcatg cgggatatcg gcattcccaa
     2581 cggcctcacc gccatcggct acaccgagcg cgacgtgccc gccctgatcg agggcacgct
     2641 gcgccagccg cgcctgctgt cgggggcgcc ccggccggtt ggtcagccgg agctcgagct
     2701 gatcctgcgc gacgcgatgc agtactgggg ctagcgctag agctgccccg aatcccccca
     2761 ctcggcgagc agcgtccggt aggcgggatc ctggtaacga tcgaggtgga acgcgggcgc
     2821 gtaccggggg agcggccgcc ccgcgatgta gtcggccagg agatcggcgg cgccgttcga
     2881 ggacatcatc ccgtagccgg agaggcctcc caggacgtac gcgccctcga ccggtagcgg
     2941 tcccgacagc agccggttct cctcggtctt ggtgtagtag ccgccgtcca cgaagtgctt
     3001 cggcagacgc gggacgtatg cgctcaggcc ggggatcagc cgggtcatcc cgcggatcac
     3061 gatctccgcg tacgcgggat cgaactccac cgggaagcgc ggctccatcg gctccacgtc
     3121 gtaggtccac accccgagca tcaccgggct gtctccgccg ccctccggcc ggccgtggac
     3181 gcccgcgggc atctcttcca ggaggtgccg cagcccctcg gcctgcccga gctcctcgcg
     3241 ctcgtcgtcg gaccacggca gccgcaccgg atccgtccag atcatcatcg gcgcgtggcg
     3301 tggcaccacg ccctgcgtat cgtgaaaggc gaccttggcg tgccgctcgc agaacaccgg
     3361 gatctcgacg tcgaggagcc gcccgaccgc cttgacgtac gggcccgccg cgttgacgaa
     3421 gcgtggcgtg ccgatggtgc ggccgccgcc cgcgccgctc acccgcacgc ccgtgacgcg
     3481 tccccccgcg gtctcgaccg cgtcaactcg cccctcggtc aaacgcacgc ccgcttcgcg
     3541 cgcgcgctcg agcatgtaca tgccgagctg ctggccgctg aaccagccgc agcgacgcgc
     3601 gtgcagcagc gcgaccgcct cctcggcgag gtaggggaag tggcggcgga tcaggctccg
     3661 atcggtgacc acgtccgtcc cggtgggctg cccttcgtaa ccggtcgccg gcgacggctg
     3721 atagtccccg cccggcgatc ggtgcaggcg cagcgggccg actccccgcg cggcggcgtc
     3781 ctgcccggcc ttcacgtagc cctcgatctg accgggctgg gcggtcgcga acagatagcc
     3841 ccgccgattc atcaggaaca cgttgtcgct ctcgcgcgcc agctcctcca tgaggtcgat
     3901 gctgcggttc atgagggcgg ccatgtcgtc gccgggaccc ggccaccagt tgcggtagga
     3961 ctccgccgac ttgtcgctcg tgagcgagag cggcgggcgc tcgtcgatca gcaccaccga
     4021 gcggatgccc cgccggaccg ccaggtgata ggccgccgcg acgccggcga tgccggcccc
     4081 gcagatcacg acctccgccg tcggggcgct cattggcggg acgactctac acgctttgag
     4141 cgggcaagaa aaaccggccg gcgcgtggtg gacactgtca gtgtcacgac ggtatggtga
     4201 cgctccaata cttccctgac catagtccaa gtggctgcaa ttcctgcctt gccgatccag
     4261 gcacgtcggt tgctccaagc gcgcccatga tgacacgaca gagcgaccgc cgccgccatc
     4321 cccgctcgga tgtctcctgg ctcgtcgtgg tggaggccgg gaagcgtcgc ttcctcctcc
     4381 agacgatgga catctcggcc cgcggcacga aggtgcggcc gcgggagcgg ctcgatatcg
     4441 gcacgccggc ccagctccgc ttccggcccc cggacgggac gccgttcaac gtgcctgcgc
     4501 tggtctggcg tacggattcc gacgggctcg ccttcctctt catgggggac atccaggacc
     4561 gtctccgccg ctacggccgc ctgctcgcca gctgagccgg cgtgagccgg cgggcggccg
     4621 ctacttcttg aaccagtccc agaccttgtc ccaccagcgg ccgatctgct ccgacacgcc
     4681 cccgtgctcc tcgcacggcg gcggctccgt cccggtcagg aagacctcgg tgaccacgac
     4741 cgggcagtac gccgtcgcct tccgcccgtt ggtcgcgtcg atcttcgcgt aggtgacgcc
     4801 cggcggctcc gggaacgcgc cgcccggata gatgtcgagg gcctggcgca tgaactccga
     4861 ccagatcggg agcgcgccct ccgccccgga cagtcccgcc ggcgctccgt cgtcgaagcc
     4921 cacccagacc agggccagca ggttcgacga gtagcccacg aaccaggcgt cgcgcccgtc
     4981 gttcgtggtg ccggtcttgc ccgccaccgc gccgggcacg cccagcgtgc gcgccgaagc
     5041 gcccgtcccc gagttgatca cgccctcgag gagcgaggtg acgagaaacg cctccggcgc
     5101 gccgatcacc ggccgcgtct cccggctcgc gctccagatc gcgcggcccg agtcgtcggt
     5161 gacggtgtcg accacgcccc cgctcggcgc gatcccgccg ttcgcgagcg gtaggtacgc
     5221 gcgcgcgaga tcgagcggcg tggtctcgaa cacgccgagg gccagcgcgg gcaccggctt
     5281 gaggtcgcct tccaccccca ggctccgcgc ggtgtcgacc acggtgttca ggcccaccgc
     5341 ctgggcgagc cgcaccgtcg cggtgttgag cgagccctcg agcgcccgcc gcaccgtcac
     5401 ctgcccctga tagcggtcgt cgtagttctt ggggctccac ggcttgccgt ccaccacgat
     5461 cgtgatcggc gcgtcctcga tcagcgacgc ggccgtgaag cgcggcccgt ccggccgcgg
     5521 ggtcagggcg gcgaggaaca cgaagggctt gaaggccgag cccggctggc ggcgcgcgag
     5581 caccgcgcgg ttgaactggc tgagccggta gtcgcggccg cccacgagcg cgcgcacctg
     5641 gccggtggag gtatcgagca cgatgagagc ggcctgcaag cgctcctccg ggctcgggcg
     5701 gcgcaggcgc ggccgcgagg tctcgagtcg atcgattccc ttgaccaccg cggcctcggc
     5761 gaggcgctgc agcaccgggt cgagcgtggt gtagacccgc accccgtgct ggtcggccag
     5821 atccatctcg gtggagcgct cgagatccgc gcggacgtag tcgacgaagt agggcgccac
     5881 cagcccgctg ccgggcgtga tgcgggcctt caccggctcg cggcgcgcgc gccggtagtc
     5941 cgcctccgag atcttgccca ggtcgcgcag gcgcgtcagc accacgtcgc gccgctcccg
     6001 ggcccggtcg ggattggcga ccggcgagta gttgttgggg ccccggatca tcccggccag
     6061 gagcgccgcc tccgggaggg tcagctggtg gacctccttg ccgaagtagg cgcgcgaggc
     6121 cgcgccgacc ccgcgcaccg ccgagccgcc cgtctgtccc aggtagatct cgttcaggta
     6181 cgcctcgagg atctggtcct tcgagtaccg ccactcgagc gcggtggaga gcagggcctc
     6241 gttgagcttg cgcgaatacg tccgcgcggg ggtgaggagc cggctcttca cgagctgctg
     6301 ggtgatggtg ctgccgccct cgacgacgcg ccccttgcgg acgttggccc agagcgcccg
     6361 cagcaccccg cgcgggtcga gcccgccgtg ctcgtagaag cgctcgtcct cgatggccag
     6421 gacggcggtg cgcaacaccg gaggcacgtc ggtcaggcgc accgggcgaa tgctctctcc
     6481 catgccggcg cccgcgctcg cgaggagctc gggcgggagc gccacgctct gcacctcggt
     6541 cccgttctgg cgcaaccggg tgatccgtcc acccgacacg gtgagactca cccggcccgc
     6601 gccgccggcc ccggagcccg cgtcgatgtc ccaggcgctg tcggatcgag agaactgtcc
     6661 cggccccgcg gcggtgcggc tctcgcgata gccgagccgg ccgaggatgc cggccaggtc
     6721 gagggcgccg atcgagacgc ccgaccgcag caccggcggg gccgcataga gaagtgtcga
     6781 gcggcgcgcc tcgacgcgct cgaacttgga gagggcgcgc agcgagtaga tggcgagccc
     6841 gccgcccacc acgatcagga tcacgagcag cgcggccgcg aacagcgtga gccgccgcag
     6901 gacccggaag cggccctccc acaacgcggc catcacgcgt ccgcgcgcac gctcggggag
     6961 aggggtgagc ggctcaggag ccggcgcccc tccgcagggt gaccatcacc agccgggcca
     7021 cctcgggatc gtccgacagc tcgaccacca cgaaatcgcc gatgcggacg tctccgagcg
     7081 tgatggtgct gtcacggaac gcgtccttca gatcgcgccc cgcctggttc cgctgcgacc
     7141 agacgacgag cgcgtcgcgc ggcacctgga accggagggc gcggcgctcg ccgccgactc
     7201 cgaactcgtc gacggtgagc gtgcgcgcct gcggatccac cgccagcacc gtccccgcgt
     7261 gtcgctcccc cgcgggcgcc ggggccggcg tcccggtcag ggcaatcagg ctcatcagca
     7321 gcgcggccag ggcgcttccg aactttgcaa atgcccgcac cggggcaagc ccccacaccg
     7381 ggcgcatcac gaccggggtg aggggggagc ccccacctcg gccgccttca tcttccggac
     7441 cagctcggcg tacgaggagg tctggatgat cttgtcgaac t
//
LOCUS       DATMCV010000047         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_402234, whole genome shotgun sequence.
ACCESSION   DATMCV010000047 DATMCV010000000
VERSION     DATMCV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402234"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..268
                     /locus_tag="VNU03_01215"
     CDS             95..268
                     /locus_tag="VNU03_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73093.1"
                     /translation="MNRRSPLFITASLLIATLSVALPDLDLETVTETQVREAAAHDAT
                     GPRPASTARETTP"
     gene            265..555
                     /locus_tag="VNU03_01220"
     CDS             265..555
                     /locus_tag="VNU03_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73094.1"
                     /translation="MIAPLGIAGRIAGLAVGLGLLAACTTSQSALSYRKPGVVADAQQ
                     QDEQACLRSSSGPNDQGYLLLPFEIDGPAFRRCMEVRGYVLEPTAASRPARP"
     gene            582..953
                     /locus_tag="VNU03_01225"
     CDS             582..953
                     /locus_tag="VNU03_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73095.1"
                     /translation="MIKQALWSACFLAAVAAAPAAGTAQGPTDGPLLRLQWQADPARD
                     GVQAVCGEVLNDGGLAADHVRLLVEELDANGRVINSRELEVLGEVPADGRSYFCVPEP
                     AGAATYRLSVTGADSMYTRGQ"
     gene            complement(991..1398)
                     /locus_tag="VNU03_01230"
     CDS             complement(991..1398)
                     /locus_tag="VNU03_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73096.1"
                     /translation="MAALIAGCAGPTAGPSRAAPAAFTPDVVGTWNGVFWWLGGNYWE
                     DEGTLLLQIKEDGTFTVTMTPTPAANNIAKPTSWSGTVSQSGRLVVFHLSKGAWPAWS
                     SLARSGDALYGVANDPATGADIQVSFVRAGART"
     gene            1672..>2138
                     /locus_tag="VNU03_01235"
     CDS             1672..>2138
                     /locus_tag="VNU03_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HWP73097.1"
                     /translation="MDLSRRDFFKLSAGSLTGTAVGGIAGLGASLAPALAPAQTLRIE
                     KARATPSVCPYCSVGCATLVHTVGDKIVNIEGDPRSPHNEGTLCPKGAAIYQLHINPN
                     RPTKVLHRAPGAADWEVWDLDRAIGRVAELVKKTRDETFIERLEDGRLVNMTPA"
BASE COUNT          332 a          786 c          726 g          294 t
ORIGIN      
        1 gagcgccgcc ccgacgcagt cgatcggagc gactcttccg caagaaccgg ataaccggga
       61 ggaaccgacg tccgggctga gtcgtctcat gggcatgaac cgacggagtc cactcttcat
      121 caccgcgtcg ctgctcatcg cgacgctctc ggtggcgctg cccgacctcg acctcgagac
      181 ggtgacggag acgcaggtgc gggaggcggc ggcgcacgac gccaccggtc cccgtcccgc
      241 gtcgaccgcg cgggagacaa caccatgatc gctcccctcg gaatcgcggg acggatcgcc
      301 gggctcgcgg tgggcctcgg cctgctggcc gcctgcacca cgtcgcagtc cgcgctcagc
      361 tatcgcaagc cgggtgtggt cgccgacgcc cagcagcagg acgagcaggc ttgcctgcgc
      421 tcgtcgagcg ggcccaacga ccagggctac ctcctgcttc cgttcgagat cgacggcccg
      481 gccttccgcc gctgcatgga agtgcgcggc tacgtgctgg agcccaccgc ggcctcgcgg
      541 ccggcccggc catagcccgt cgtccacagg aggacacgag catgatcaag caagctctct
      601 ggagtgcgtg cttcctggcg gcggtggccg ccgccccggc cgcgggaacg gcccaggggc
      661 cgaccgatgg ccccctgctc cgcctgcagt ggcaggcgga cccggcccgt gacggcgtgc
      721 aggcggtctg cggagaggtg ctcaacgacg gcgggctcgc cgccgaccac gtccgtcttc
      781 tcgtcgagga gctggacgcc aacgggcgcg tgatcaacag ccgggagctc gaggtcctgg
      841 gagaggtacc cgccgacggc cgctcctact tctgcgtgcc cgagcccgcg ggcgccgcga
      901 cgtaccggct gagcgtcacc ggcgccgact cgatgtatac ccgcggccag tagccgagcc
      961 gggcggccca gcgcgcggcg gacgggcctc tcaggtccgt gcccccgcgc gcacgaagct
     1021 gacctggatg tccgctccgg tcgccgggtc gttggccacc ccgtacagcg cgtctccgga
     1081 gcgggcgagc gacgaccatg ccggccacgc tcccttcgaa aggtgaaaga cgacgagcct
     1141 tccgctctga ctcacggtgc cggaccagct cgtcggcttc gcgatgttgt tcgcggccgg
     1201 cgtcggcgtc atcgtcacgg tgaaggtccc gtcctccttg atctggagga gcagtgtgcc
     1261 ttcgtcctcc cagtagttgc cgccgagcca ccagaacacg ccgttccagg tcccgacgac
     1321 gtcgggggtg aacgcggccg gagctgcccg ggacgggccg gcggtgggtc ccgcgcagcc
     1381 ggcgatgagc gcggccaata cgagcccgaa gagcgctgga gcgactgtac gttgccgcat
     1441 cggacggttc ctccctgctc tgtagatgcg acgggccgag cccggagtct aatcaccctc
     1501 cctttccgca agagcgcgac actcgaggaa ccactcatcc cgacccagca ccttccggcg
     1561 gcccttgccg atccttgcga aaagcttcgc cgacgccttc gcggcgcgcc ggcgggagat
     1621 caaaaccgac gacggctctg cacgagccga tcctcccgac gaggagcgcc catggacctg
     1681 tcacgacgag acttcttcaa gctatccgcc ggcagcctca ccggcaccgc ggtcggcggg
     1741 atcgccgggc tcggcgcgag cctggcgccg gccctcgccc cggcgcagac gctccggatc
     1801 gagaaggccc gggccacgcc gagtgtctgc ccctactgct cggtgggctg cgccaccctc
     1861 gttcacaccg tgggcgacaa gatcgtcaac atcgagggcg atccccggag cccgcacaac
     1921 gagggcacgc tctgtccgaa gggcgccgcg atctaccagc tccacatcaa cccgaaccgt
     1981 cccaccaagg tcctgcaccg ggcgccgggg gccgccgact gggaggtgtg ggatctcgac
     2041 cgggcgatcg gccgggtggc cgagctggtc aagaagaccc gcgacgagac gttcatcgag
     2101 cggctcgaag acggcaggct cgtcaacatg acgccggc
//
LOCUS       DATMCV010000048         2252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_535206, whole genome shotgun sequence.
ACCESSION   DATMCV010000048 DATMCV010000000
VERSION     DATMCV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535206"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VNU03_01240"
     CDS             <1..881
                     /locus_tag="VNU03_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HWP73098.1"
                     /translation="SDGHWGGAILGYALVFFAIWWAWMNFTWFASAYDADDAPYRLAV
                     LVQIAGVLVLAAGVPRAFEQRDFHVATAGYAIMRIGLVALWLRAAASDRAGRRTAIRY
                     AIGLTLVQVGWLALLALPADRWLAGWLVLGPAELLVPVWAERHRPTSWHPHHIAERFG
                     LLTLIVLGESVLSASMAIQAAVDTATVTPALLSVIGGGIVLLFAMWWLYFDEPAHHVL
                     VSSRAAFVWGYGHLVVFASTAAVGAGLAVAADYATGRAHLSAGAALAVVAVPLALYAA
                     SVWTLVIRPVRRASFR"
     gene            complement(944..1270)
                     /locus_tag="VNU03_01245"
     CDS             complement(944..1270)
                     /locus_tag="VNU03_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWP73099.1"
                     /translation="MDTVLRKSGAVLAVLAVMLLMVACQSTTGRTLGENIDDTGITTA
                     VKAKLAAEKISTLTRIDVDTNQGVVALNGTVQTEAMKVRAEQITRQVKGVRDVINNLR
                     IQAAAR"
     gene            complement(1270..1827)
                     /locus_tag="VNU03_01250"
     CDS             complement(1270..1827)
                     /locus_tag="VNU03_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73100.1"
                     /translation="MLETERGGMQVYETALLCVVNDELKEEWEKYLDQTKSHERIVLE
                     LMDKLGLDPARETPGRGVVRLIGESLVEAMEMARKEGSAEAAQLVACEVVVQAETKDH
                     LNWELLHEASEKAKGDDRKALVAAYEQVEEQEDEHLYHSTGWCRELWIESLGLPAVLP
                     PPEEEKEVKTAIGAARAKKARKEML"
     gene            complement(2063..>2252)
                     /locus_tag="VNU03_01255"
     CDS             complement(2063..>2252)
                     /locus_tag="VNU03_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73101.1"
                     /translation="SRIVHLKKRGDLYKQVQKLVVDDAPWIFIDNAVQNAAGSKKVDG
                     FKLHPSFYLFFDKISVAP"
BASE COUNT          276 a          798 c          759 g          419 t
ORIGIN      
        1 tgagcgacgg gcactggggc ggagcgatcc tgggctacgc gctcgtcttc ttcgcgatct
       61 ggtgggcgtg gatgaacttc acctggttcg cgtcggccta cgacgcggac gacgctccct
      121 accgcctggc cgtcctggtc cagatcgcgg gggtgctggt tctcgccgcg ggggtgcccc
      181 gcgcgttcga gcagcgcgac ttccacgtcg ccaccgccgg ctacgcgatc atgcgcatcg
      241 ggctggtcgc gctgtggttg cgggccgcgg cttcggatcg ggcgggccgg cgcaccgcga
      301 tccgctacgc gatcgggctc acgctggtcc aggtgggctg gctcgcgctc ctcgcgcttc
      361 cggccgaccg ctggctggcc ggctggctcg tgctgggccc cgcggagctg ctggtgcccg
      421 tctgggccga gcggcaccgg ccgaccagct ggcaccccca tcacatcgcg gagcggttcg
      481 ggctcctcac cctcatcgtg ctcggcgagt cggtgctttc ggcgtccatg gcgatccagg
      541 ccgcagtgga cacggccacc gtcactcccg ccctgctctc ggtgatcggg ggcgggatcg
      601 tcctcctgtt cgcgatgtgg tggctctact tcgacgagcc ggcccatcac gtgctggtga
      661 gcagccgcgc cgccttcgtc tggggttacg gccacctggt ggtcttcgcc tccaccgccg
      721 cggtgggcgc cgggctcgcg gtcgcggccg actacgcgac gggccgcgct catctctcag
      781 ccggggccgc gctcgcggtg gtggcggtcc cgctcgccct ctacgcggcg agcgtgtgga
      841 cgctcgtgat ccgcccggtg cgccgcgcgt ctttccggta gaccggaggc gggccgatag
      901 ccgggaggcg ggtgcgcggc cgcacccgcc tcccgggcga cgcctaccgc gccgccgcct
      961 ggatgcgcag gttgttgatc acgtcacgca cgcccttgac ctggcgggtg atctgctcgg
     1021 cgcggacctt catggcctcg gtctgcacgg tgccgttcag cgcgaccaca ccctggttgg
     1081 tgtcgacgtc gatccgggtg agggtggaga tcttctcggc ggcaagcttg gccttcaccg
     1141 cagtggtgat gccggtgtcg tcgatgttct cgcccagcgt ccggccggtg gtggactggc
     1201 aggcgaccat gagcagcatc accgccagca ccgcgaggac ggctccgctc ttgcgcagca
     1261 cggtatccat cagagcatct ccttccgggc cttcttggcg cgagccgcgc cgatggccgt
     1321 cttgacttcc ttctcctcct cgggcggcgg gaggaccgcc gggaggccca gcgactcgat
     1381 ccacagctcc cggcaccagc cggtggagtg gtagagatgc tcgtcctcct gctcctcgac
     1441 ctgctcgtag gcggcgacga gggccttccg gtcgtccccc ttggccttct cggaagcctc
     1501 gtgcagcagc tcccagttga gatggtcctt ggtctcggcc tgcaccacca cctcgcaggc
     1561 cacgagctgg gccgcctccg ccgagccctc cttccgcgcc atctccatcg cctccaccag
     1621 cgactcgccg atgagccgga ccacgccgcg cccgggggtc tcccgggccg gatcgagacc
     1681 cagcttgtcc atgagctcca gcacgatgcg ctcgtggctc ttcgtctggt ccaggtactt
     1741 ctcccactct tccttcagct cgtcgttgac cacgcagaga agcgcggtct cgtacacctg
     1801 catgcccccg cgctcggttt ccagcatctg gtagatgagt tcgttcagct gctccgtctt
     1861 cacgtctttc cccctccgtt tagagattgt cgtttcaagg gataacgcaa ggactgtgcc
     1921 tcacgatcgt cggcacggag acgggcgaat acccgccatg cgcggaccgg gcggcgggaa
     1981 ggcgaacgcg gttaccgagt agatcttgac cggcgccggc tcccggagga cccgggagcc
     2041 ggcgtggccg gggcgagcga gcctacggag cgaccgagat cttgtcgaag aagaggtaga
     2101 aagacgggtg gagcttgaac ccgtccacct tcttgctgcc ggccgcgttc tggaccgcgt
     2161 tgtctatgaa gatccacggg gcgtcgtcca ccaccagctt ctggacctgc ttgtagaggt
     2221 cgccccgctt cttgagatgc acgatgcggc tc
//
LOCUS       DATMCV010000049        10227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_135702, whole genome shotgun sequence.
ACCESSION   DATMCV010000049 DATMCV010000000
VERSION     DATMCV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135702"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VNU03_01260"
     CDS             <1..612
                     /locus_tag="VNU03_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWP73102.1"
                     /translation="GGDKLAHALATFAIEPRGRVAIDVGASTGGFTHCLLEHGAVKVY
                     AVDVGHGQLDAKLRADGRVVVMEKTNARNLPVDSFPESPDLATVDISFISLEKVLPSV
                     FGVLTSQGEAVVLVKPQFEVGKGQVGKGGVVRDGSHHRMVMARVARFAVLHGWHVRGV
                     TASPLKGPKGNREFFLLLTKTGRTLTDLDALITQVTAEEEPPA"
     gene            609..1466
                     /locus_tag="VNU03_01265"
     CDS             609..1466
                     /locus_tag="VNU03_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWP73103.1"
                     /translation="MKRLGIVAKTDREEARTVVPQLLDWCAARGIQPILEKETAGLCP
                     QATGPVVGRSELPGQSDLLLVLGGDGTLLSMARLVGDLAVPILGVNLGGLGFLTALTV
                     EELFPALEAYLRDELVIEDRMLLDARVFRQGERLGEYAALNDVVITKSAMSRIIRLEV
                     AVDGDFATGYRADGLILSTPTGSTAYCLSAGGPIVFPTMDAVVLTPICSHTLTNRPIV
                     LPATQRISITLLTDQDVMLTLDGQVGFALKANDTVEVRRATARTRLLRVPQKHFFSVL
                     RTKLKWGER"
     gene            1503..3218
                     /gene="recN"
                     /locus_tag="VNU03_01270"
     CDS             1503..3218
                     /gene="recN"
                     /locus_tag="VNU03_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWP73104.1"
                     /translation="MAVTMLRELSIRNLAVVEEAVVPFAPGLNVLTGETGAGKSIVVD
                     ALLLITGARAQLDWIRTGADTALVEAVFEIDPAGPVAALLDEAGHHPGDGQLVIKREL
                     ARSGRHRAFVNDGAATVGLLERLGELLVELHGQHEHQRLMEPARQLLLLDRFAEALDR
                     RERVGALVRTWEEARAALDRLRSEMREGARQEDLYRFQLAEIDDVRLKDGEEDELRAE
                     RSRLQHAERIVAGLQETVGLLYEEPQSATTRLSRAAALLRDLSRYEPDAAASIEAIEG
                     AQAYLEDVVGRARALRDRAVFDPERLEQIDARLDTIVKLKRKYGDSVAAILAHRQEVA
                     TALDRITRHDAIAEEMERTVAEAAAAAGAEAAALSQARTRGGERLERLIQKEIRGLGM
                     EHGRFRVALRREPAAVGELAAGPDGWRVGPRGAESAELLLSANPGEDLRPLAKVVSGG
                     ELSRVMLAAKTVLAAADDVPVLVFDEVDAGIGGRVADVVGQKLAASAAGRQVLCVTHL
                     APIAAYAGQHLLVEKRVARGATRTSVTLLPAAARVDELARMLGGERVTEASRRHAREL
                     LRDAR"
     gene            3266..6121
                     /gene="secA"
                     /locus_tag="VNU03_01275"
     CDS             3266..6121
                     /gene="secA"
                     /locus_tag="VNU03_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWP73105.1"
                     /translation="MLNAIFRSIFGTKHERDVKRMRPAVAEIAALEPGLQALSDDALR
                     ARTTELRQRLADGAQVDDLLPEAFAVVREAARRTVHMRHFDVQMMGGMVLHSGKIAEM
                     ATGEGKTLVATLPAYLNALPGKGTHVVTVNDYLARRDAQWMGPIYHALGLTVGVIQHE
                     MQYRYDPDFASPDIRLAALRPVTRKEAYAADITYGTNNEFGFDYLRDNMQFSLDDIVQ
                     REHYYAIVDEVDSILIDEARTPLIISGRDRSAESRAPLYEKVNRIIPRLKRAATIVEG
                     KLSEIEETAEGDFIVDEKSRTVSLTDQGGAYCERLLGIENLSDPAHIETAHHIHQALK
                     AHHIFHKDVDYVVKDLETVDDQGRASLQPQVIIVDEFTGRMMPGRRWSDGLHEAVEAK
                     EGLKIARENQTLATITLQNYFRMYDKLSGMTGTAATEAEEFAKIYELDVTVIPTNRTL
                     GRVNFPDVVYKSEREKFDAVANEIAERHGAGQPVLVGTVSIEKSERLSKLLKKRGIPH
                     QVLNAKYHEREAEIVAQAGREKSVTIATNMAGRGTDILLGGNPDFLAKALLSKKGLDP
                     ATAPAEAREASWLEAKKSTDPEHERVVALGGLHIIGTERHESRRVDNQLRGRSGRQGD
                     PGSSRFYLSLEDDLLRIFGSERIQRIMDRLGMEEGEPIEHKLVTRAIGTAQKRVETRN
                     FEIRKHLLEYDDVMNKQREIVYGLRREVLAGESQEETLLEWIGDLAGSVVERYAAADA
                     HPEDWDLVGLNEALHRQFDFRVPTAALEDVSAQPALIDEITAAAERHYREREQQMGAD
                     MFHRLERWIVLGLEWEGGGFRGINQLWREHLLNMDHLKEGIGLRGYGQRDPLTDYKKE
                     AFDMFQEMIGSLKETAVEQLFKVRMTQEDAPTTRVAQRTPEWQETRGGEQGDAAPRAA
                     ASRNEPRAADGQKVGRNDACPCGSGKKYKKCCLLKGA"
     gene            6251..9808
                     /gene="smc"
                     /locus_tag="VNU03_01280"
     CDS             6251..9808
                     /gene="smc"
                     /locus_tag="VNU03_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HWP73106.1"
                     /translation="MRLESILIHGFKSFAEKTDVKVFPGVTCIVGPNGCGKSNVADAM
                     RWALGEQSPKTLRGNKMEDVIFHGSASRKMVGMAEVSLVFNNDGGLNVPWSEVAVARR
                     LYRTGESEYLLNTNVCRLRDVQDLFAGTGVNPKAYALMDQERLNHVLTAKPWERRLFI
                     EEAAGIARYKQQRSETQGKLDATRLNMQRLRDVMEEVRRQLSSIERQARKAQQYKALQ
                     SERQGLDLALVAADYAALATQHDTVTREMESLRETEEQQRARVATLTGRQALQAAAIQ
                     DTEYRLGDLRQSVQKIQGEAERLLERREQMGLQIQDLLAEETRLEEEIRGLAERRVTL
                     SAEQDDKSRALDAARQRHGRQDEEVRTVEAQIEACRSTLGERRERFEALRLEQIRVAG
                     ARADLTRSSGELRERRTQVERRADRLAAELATARSEADTLAATRLRLETARQRTGAQL
                     SLLSAELQEIDSARRDHETVRTAAQEALAGLRVKLAGRQSALEALDRLEREREGYGAG
                     VRAMFARTASGDLGGVVGTVADLLEVPNGLEAAVEAVLGDRLQWVVVERFEHARAALG
                     YLEREGVGAATLLPLETLPSPAAVPEDSGEVSWATRLVGGPRPALLHYLLGRVGVVAH
                     LDQAETLWRRNGVIATYVTRSGEVLSPSGRLTGGHRGSERQSNDQSLLGRKRAIRQLV
                     EELRGLRRDVDTAATHLETVEGEVAGLRTRLEGIQSDYQAHETARLSGEKDLESVQRE
                     SDRIARHLETLAAENDQLAGERDETASQLLDLERALVAAAEQEAGLGGDMSALQALLE
                     SGQGDEAGLTDRLTGCRVELATVAERVDSLGRDIERIVSLERDFGLQQEQSVTRRAQT
                     AERRADLATERERTDVQARDAVTERDRLEAEVAVIAEDHQRQLGERQAVDGELREVQQ
                     QLNALVSRLHQLDLMETEGRVRREELLQEARRRHAVETAEALLAAHDVARDLEEVRQR
                     HEELSTKLEAMGPVNLVADEEFRELTERLDFLRTQHDDVVGSIKDLEKALRGMTRTAQ
                     ERFQEAFDDINRHFGDIFSRLFEGGRAELRMVPPEEGEEDPLELGVELMAQPRGKRLQ
                     AVTLMSGGEKALTGLALLFAIFYHRPSPFCVLDEVDAPLDDANIHRFLRVLRELCTQT
                     QFVVITHNRKTMEAADVLYGVTMEEPGLSRLVSVKLTEA"
     gene            9853..>10227
                     /locus_tag="VNU03_01285"
     CDS             9853..>10227
                     /locus_tag="VNU03_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWP73107.1"
                     /translation="MSLGAYLRSLREAKGGSLEDMARSTRVGIRHLEALEDERLADLP
                     SPVFVRGFIRAYCSFLRENPEPALGRYQELAGTRAAEQAAIAPPRPRNTWASSSVVVG
                     LVLLLILGVGLILVNLSVKKNGG"
BASE COUNT         1606 a         3593 c         3731 g         1297 t
ORIGIN      
        1 ggcggcgaca agctggctca cgccctcgcc accttcgcca tcgagccacg cgggcgggtc
       61 gcgatcgacg tgggagcctc caccggcggg ttcacgcact gcctgctgga gcacggagcg
      121 gtgaaggtct acgcggtgga cgtggggcac gggcagctcg acgccaagct gcgggccgac
      181 gggcgggtgg tggtgatgga gaagaccaac gcgcggaacc tgccggtgga ctccttcccg
      241 gagtcgccgg acctggccac cgtcgacatc tcgtttatct cgctcgagaa ggtgctgccc
      301 tcggtcttcg gcgtgctcac gtcccagggc gaggcggtgg tgctggtcaa gccccagttc
      361 gaggtgggca agggccaggt cggcaagggg ggcgtggtgc gggacggctc gcaccatcgg
      421 atggtgatgg cccgggtcgc ccgcttcgcg gtgctgcacg gctggcacgt gcgcggggtc
      481 accgcctcac cgctgaaggg gcccaagggc aatcgcgagt tcttcctgct gctgaccaag
      541 accgggcgca ccctgaccga cctggacgcc ctgatcaccc aggtgaccgc ggaggaggag
      601 cctcccgcgt gaagcggctg ggcatcgtcg ccaagaccga ccgggaggaa gcgcgcaccg
      661 tcgtgcccca gctgctcgac tggtgcgccg cgcgcggaat ccagccgatc ctcgagaagg
      721 agaccgcggg cctctgcccg caggccaccg ggccggtggt cggccggtcg gagctgccgg
      781 gccagtccga tctcctgctc gtgctcggcg gcgacggcac gctcctcagc atggcgcgcc
      841 tggtgggaga cctggccgtg ccgatcctcg gggtcaacct gggcgggctc ggcttcctca
      901 ccgccctcac cgtcgaggag ctgttcccgg cgctcgaggc ctacctgcgc gacgagctcg
      961 tcatcgagga ccgcatgctg ctggacgccc gcgtcttccg ccagggcgag cggctcgggg
     1021 agtacgcggc gctcaacgac gtggtcatca ccaagtcggc gatgagccgt atcatccggc
     1081 tggaggtggc ggtggacggc gacttcgcca ccgggtaccg cgcggacggg ctcatcctct
     1141 cgacgcccac cggctcgacc gcctactgcc tctcggcggg cgggcccatc gtgttcccga
     1201 ccatggacgc ggtcgtgctc acgccgatct gctcacacac cctcaccaat cgcccgatcg
     1261 tgctgcccgc gacccagcgc atctcgatca ccctgctgac cgaccaggac gtgatgctca
     1321 ccctggacgg acaggtgggc ttcgcgctca aggccaacga caccgtcgag gtgcggcgcg
     1381 ccaccgcgcg cacgcggctc ctccgggtgc cgcagaagca cttcttctcg gtcctccgga
     1441 ccaagctcaa gtggggggag cgataaggat acagttgagc gccgccgggg cggggccgta
     1501 gcatggcggt gacgatgctg cgcgagctgt ccatccgcaa cctggccgtg gtcgaggagg
     1561 cggtggtgcc cttcgccccc ggcctcaacg tgctcaccgg cgagaccggc gccggcaagt
     1621 ccatcgtggt ggacgcgctg ctcctcatca ccggcgcgcg cgcccagctc gactggatcc
     1681 gcaccggcgc cgacaccgcg ctggtcgagg cggtcttcga gatcgacccg gcgggaccgg
     1741 tggccgccct gctcgacgag gccggtcacc atccgggcga cggccagctc gtcatcaagc
     1801 gggagctggc ccggtccggg cgtcatcgcg ccttcgtcaa cgacggggcc gccaccgtgg
     1861 gcctgctcga gcgcctgggc gagctgctcg tggagctgca cggccagcac gagcaccagc
     1921 gcctgatgga gccggcccgc cagctcctgc tgctggaccg cttcgcggaa gccctcgacc
     1981 gccgggagcg cgtgggggcg ctggtgcgca cgtgggaaga ggcgcgcgcc gccctcgatc
     2041 gccttcggag cgagatgcgg gaaggggccc ggcaggagga cctctaccgg ttccagctcg
     2101 ccgagatcga cgacgtccgc ctgaaggacg gcgaggagga cgagctgcgg gccgagcggt
     2161 cacgcctgca gcacgcggag cggatcgtgg ccgggctgca ggagacggtc ggcctgctct
     2221 acgaggagcc gcaatccgcc accacgcggc tcagccgcgc ggccgcgctc ctgcgcgacc
     2281 tctcgcgcta cgagccggac gcggccgcct ccatcgaggc catcgagggc gcgcaggcct
     2341 atctggagga cgtggtgggc cgggcgcggg ccctgcggga ccgagcggtc ttcgaccccg
     2401 agcggctcga gcagatcgac gcgcggctcg acaccatcgt caagctcaag cgcaagtacg
     2461 gggactcggt ggccgcgatc ctcgcccacc ggcaggaagt cgcgaccgcg ctcgaccgca
     2521 tcacgcgcca cgacgcgatc gcggaggaga tggagcgcac ggtggccgag gcggcggccg
     2581 cggcgggggc ggaggccgcc gcgctctccc aggcccgcac ccgcgggggc gagcgcctcg
     2641 agcggctcat ccagaaggag atccgcgggc tcgggatgga gcacggccgc ttccgcgtgg
     2701 ccctccgccg cgagccggcg gcggtgggcg agctcgccgc ggggccggac ggctggcggg
     2761 tgggcccgcg cggcgccgag agcgccgagc tgctgctctc cgccaacccc ggcgaggacc
     2821 tgcgcccgct cgccaaggtc gtctcgggcg gcgagctgtc ccgggtgatg ctggccgcga
     2881 agacggtcct cgccgcggcc gacgacgtgc cggtgctggt gttcgacgag gtggacgcgg
     2941 ggatcggcgg ccgcgtggcg gacgtggtgg gccagaagct cgcggccagc gcggcgggcc
     3001 gccaggtgct ctgcgtgacc catctggccc ccatcgcggc gtacgcgggc cagcatctgc
     3061 tggtggagaa gcgcgtggcc cgcggcgcga cgcggaccag cgtgaccctg ctgcccgcgg
     3121 cggcccgagt ggacgagctg gcccgtatgc tggggggcga gcgggtcacc gaggcgtccc
     3181 gccggcacgc ccgcgagctc ctgcgggacg cgcgctgacc caggccggcg gccgtgcctc
     3241 gcgggtggtg taagatgagg ccctgatgct gaacgcgatc ttccgctcga tcttcggcac
     3301 caagcacgag cgcgacgtca agcgcatgcg cccggcggtg gccgagatcg ccgccctcga
     3361 gcccgggctc caggccctct ccgacgacgc gctccgcgcc cgcaccaccg agctgcggca
     3421 gcggctggcc gacggcgccc aggtcgacga cctgctgccc gaagccttcg cggtggtccg
     3481 cgaggccgcg cgccggaccg tccacatgcg ccacttcgac gtccagatga tgggcggcat
     3541 ggtgctccac tccgggaaga tcgcggagat ggcgaccggc gagggcaaga ccctggtggc
     3601 cacgctcccc gcctatctca acgcgctccc cggcaagggg acccacgtgg tcacggtcaa
     3661 cgactacctg gcccggcgcg acgcccagtg gatggggccg atctaccacg cgctgggcct
     3721 gaccgtcggg gtgatccagc acgagatgca gtaccgctac gatcccgact tcgcctcgcc
     3781 cgacatccgg ctcgccgccc tccgcccggt cacccggaag gaggcctacg cggccgacat
     3841 cacctacggg accaacaacg agttcggctt cgactacctc cgcgacaaca tgcagttcag
     3901 cctcgacgac atcgtccagc gcgagcacta ctacgccatc gtggacgagg tcgactcgat
     3961 cctgatcgac gaggcgcgca ccccgctgat catctcgggg cgcgaccgga gcgcggagag
     4021 ccgggccccc ctctacgaga aggtcaaccg gatcattccg cggctcaagc gggccgccac
     4081 catcgtggag ggcaagctct ccgagatcga ggagaccgcg gagggcgact tcatcgtcga
     4141 cgagaagtcg cgcaccgtct ccctgaccga ccagggcggc gcctactgcg agcggctgct
     4201 cggcatcgag aacctgtccg accccgctca catcgagacc gcccaccata tccaccaggc
     4261 gctcaaggcc caccacatct tccacaagga cgtggactac gtggtcaagg acctggagac
     4321 ggtggacgac caggggcggg cgtcgctcca gccccaggtc atcatcgtgg acgagttcac
     4381 cggccgcatg atgcccggcc ggcgctggtc cgacggcctg cacgaggcgg tggaggccaa
     4441 ggaagggctc aagatcgcgc gggagaacca gaccctcgcc accatcacgc tgcagaacta
     4501 cttccggatg tacgacaagc tctccggcat gaccggcacc gcggccaccg aggccgagga
     4561 gttcgccaag atctacgagc tcgacgtcac ggtcatcccg accaaccgca cgctgggccg
     4621 ggtgaacttc ccggacgtgg tgtacaagag cgagcgcgag aagttcgacg cggtcgccaa
     4681 cgagatcgcg gagcggcacg gggcgggcca gccggtgctg gtgggcaccg tctccatcga
     4741 gaagtcggag cgcctctcca agctgctcaa gaagcgcggg atcccccacc aggtgctcaa
     4801 cgccaagtac cacgagcgcg aggccgagat cgtggcccag gccgggcgcg agaagtcggt
     4861 caccatcgcc accaacatgg ccggccgcgg caccgacatc ctgctcggcg gcaaccccga
     4921 tttcctcgcc aaggccctgc tcagcaagaa ggggctcgac ccggccaccg cgcccgcgga
     4981 ggcgcgcgag gcctcctggc tggaggcgaa gaagtcgacc gatcccgagc acgagcgggt
     5041 ggtggccctg gggggcctgc acatcatcgg caccgagcgc cacgagtcgc ggcgggtcga
     5101 caaccagctc cgcgggcgct ccgggcgcca gggcgacccc ggctcctcgc gcttctacct
     5161 ctcgctcgag gacgacctgc tgcgcatctt cggctccgag cggatccagc gcatcatgga
     5221 ccgcctcggc atggaagagg gcgagccgat cgagcacaag ctggtcaccc gcgcgatcgg
     5281 caccgcccag aagcgggtgg agactcgcaa cttcgagatc cgcaagcacc tgctcgagta
     5341 cgacgacgtg atgaacaagc agcgggagat cgtctacggg ctgcggcgcg aggtcctggc
     5401 cggggagagc caggaggaga ccctgctcga gtggatcggc gacctcgcgg gcagcgtggt
     5461 cgagcggtac gcggccgccg acgcccaccc cgaggactgg gacctggtcg ggctcaacga
     5521 ggcgctccac cgccagttcg atttccgggt gccgaccgcc gcgctggagg acgtgtcggc
     5581 gcagccggcc ctgatcgacg agatcaccgc ggcggcagag cgtcactacc gcgagcgcga
     5641 gcagcagatg ggcgccgata tgttccaccg gctcgagcgc tggatcgtcc tcgggctgga
     5701 gtgggagggc ggggggttcc gcggaatcaa ccagctctgg cgcgagcacc tgctgaacat
     5761 ggatcacctg aaggaaggca tcgggctgcg cggctacggc cagcgcgatc cgctgaccga
     5821 ttacaagaag gaagccttcg acatgttcca ggagatgatc ggcagcctga aggagacggc
     5881 ggtcgagcag ctcttcaagg tgcgcatgac gcaggaggac gcgcccacca cgcgggtggc
     5941 ccagcgcacc cccgagtggc aggagacgcg cggcggggag cagggcgacg cggcgccgcg
     6001 cgcggccgcg agccggaacg agccccgcgc ggccgatggg cagaaggtcg gccgcaacga
     6061 cgcgtgcccg tgtggctccg gcaagaagta caagaagtgc tgcctgctca agggggccta
     6121 gcgccccggt tcggtccacc caccgggtag tgccgccgcg gccccgcggc ccccactcgt
     6181 gggggttttt tcttggttga tcccccgacc gtgtgttatt gatgaaatac gccctgcggg
     6241 cgtgccagtc atgcgactcg aatcgatcct gatccacggc ttcaagtcgt tcgccgagaa
     6301 gaccgacgtg aaggtcttcc ccggggtcac ctgcatcgtc ggcccgaacg ggtgcggcaa
     6361 gtccaacgtg gccgacgcga tgcgctgggc cctgggcgag cagagcccga agaccctgcg
     6421 cggcaacaag atggaagacg tcatcttcca tggctcggcc tcccgcaaga tggtgggcat
     6481 ggccgaggtc agcctcgtct tcaacaacga cggcggcctc aacgtgccgt ggagcgaggt
     6541 ggcggtggcc cggcgcctgt accgcaccgg ggagagcgag tacctgctca acaccaacgt
     6601 gtgccgcctg cgggacgtgc aggatctctt cgcgggcacc ggggtcaatc ccaaggccta
     6661 cgcgctcatg gaccaggagc gcctcaacca cgtgctgacc gccaagccgt gggagcgccg
     6721 gctgttcatc gaggaggcgg cgggcatcgc ccgctacaag cagcagcgct ccgagaccca
     6781 gggcaagctc gacgcgaccc ggctcaacat gcagcggctg cgcgacgtga tggaggaggt
     6841 gcggcggcag ctgtcctcga tcgagcgcca ggcccggaag gcccagcagt acaaggcgct
     6901 ccagtccgag cggcaggggc tcgacctggc cctcgtcgcc gccgactacg cggcgctcgc
     6961 cacccagcac gacacggtca cgcgcgagat ggagtcgctg cgcgagacgg aggagcagca
     7021 gcgggcgcgg gtggcgaccc tgaccgggcg gcaggccctg caggcggcgg cgatccagga
     7081 caccgagtac cggctcgggg acctgcgtca gtcggtgcag aagatccagg gcgaggccga
     7141 gcggctgctc gagcggcgcg agcagatggg gctgcagatc caggacctgc tcgcggagga
     7201 gacccggctc gaggaggaga tccgcgggct cgcggagcgg cgggtcaccc tctccgccga
     7261 gcaggacgac aagagccgag cgctcgacgc ggctcgacag cggcacggcc ggcaggacga
     7321 ggaggtccgg acggtggagg cgcagatcga ggcgtgccga tccacgctcg gcgagcggcg
     7381 ggagcgcttc gaggccctgc ggctcgagca gatccgggtg gcgggcgccc gtgccgatct
     7441 gacgcgctcg agcggggagc tgcgcgagcg gcgcacccag gtcgaacgcc gcgccgaccg
     7501 gctcgcggcc gagctggcca ccgcccgcag cgaggccgac acgctcgccg ccacgcgcct
     7561 gcgcctggaa accgcgcggc agcggaccgg cgcccagctc tcgctactca gcgccgagct
     7621 gcaggagatc gacagcgcgc gacgggatca tgagacggtc cggaccgcgg cccaggaggc
     7681 gctcgcgggc ctgcgggtga agctcgcggg gcgccagtcg gccctggagg cgctcgaccg
     7741 cctcgagcgg gagcgcgaag gctacggcgc cggcgtgcgc gccatgttcg cgcgcaccgc
     7801 ctcgggagac ctgggcgggg tcgtggggac cgtcgccgat ctcctggagg tgccgaatgg
     7861 gctcgaggcg gcggtcgaag ccgtcctcgg ggaccgcctg cagtgggtgg tggtggagcg
     7921 gttcgagcac gcccgcgccg cgctcggcta tctcgagcgg gaaggggtgg gcgcggcgac
     7981 gctcctgccg ctcgagacgc tgccctcgcc cgccgcggtg ccggaggact cgggcgaggt
     8041 gagctgggcg acccggctgg tgggcggccc gcggccggcc ctgctccact acctgctggg
     8101 gcgggtgggg gtcgtcgccc acctcgacca ggcggagacc ctgtggcgcc gcaacggggt
     8161 gatcgcgacc tacgtcacgc gctcgggcga ggtgctctcg ccgagcggcc ggctcaccgg
     8221 tgggcaccgc ggcagcgagc ggcagagcaa cgatcagtcg ctcctcgggc gcaagcgcgc
     8281 catccgccag ctcgtcgagg agctgcgggg cctccggcgc gacgtcgaca ccgcggcgac
     8341 gcacctcgag acggtggaag gcgaggtggc cgggctgcgc acgcgcctcg aagggatcca
     8401 gtccgactac caggcgcatg agaccgcgcg gctgtccggc gagaaggatc tcgagtcggt
     8461 gcagcgcgag tcggaccgga tcgcccggca cctggagacc ctcgcggccg agaacgacca
     8521 gctcgcgggc gagcgcgacg agaccgcctc gcagctgctc gatctggagc gcgcgctggt
     8581 ggcggccgcc gagcaggagg cgggcctggg cggcgacatg agcgcgctcc aggccctgct
     8641 cgagagcggg cagggggacg aggccgggct gaccgaccgc ctcaccggct gtcgcgtcga
     8701 gctggccacg gtggccgagc gggtcgactc gctcggtcgc gacatcgagc ggatcgtgag
     8761 cctcgagcgc gatttcggcc tgcagcagga gcagtcggtg acccgccgcg cgcagaccgc
     8821 ggagcggcgg gccgacctgg ccaccgagcg cgagcggacc gacgtccagg cgcgcgacgc
     8881 ggtgaccgag cgcgaccggc tcgaggcgga ggtcgcggtc atcgcggaag accaccagcg
     8941 gcagctcggg gagcggcagg cggtcgacgg agagctgcgc gaggtgcagc agcagctgaa
     9001 cgcgctcgtg agccgcctcc accagctcga cctcatggag acggaggggc gggtgcgccg
     9061 cgaggagctg ctgcaggagg cgcggcgccg gcacgcggtc gagaccgcgg aggccctgct
     9121 ggccgcccac gacgtggccc gggacctgga ggaagtacgc cagcggcacg aagagctctc
     9181 caccaagctc gaggcgatgg gtcccgtgaa cctggtcgcc gacgaggagt tccgcgagct
     9241 caccgagcgc ctcgacttcc tccgcaccca gcacgacgac gtggtcggct ccatcaagga
     9301 tctggagaag gccctccgcg gcatgacccg caccgcccag gagcggttcc aggaggcctt
     9361 cgacgacatc aaccggcact tcggcgacat cttctcccgc ctcttcgagg gtgggagggc
     9421 cgagctgcgg atggtgccgc cggaggaggg cgaggaagat cccctcgagc tgggggtgga
     9481 gctgatggcg cagccgcgcg gcaagcgcct gcaggcggtc accctcatgt cgggcggcga
     9541 gaaggccctc accggcctcg ccctgctctt cgcgatcttc taccaccggc cgagcccgtt
     9601 ctgcgtgctg gacgaggtcg acgcgcccct cgacgacgcc aacatccacc gcttcctccg
     9661 ggtgctgcgg gagctgtgca cccagaccca gttcgtggtg atcacccaca accgcaagac
     9721 catggaagcc gccgacgtgc tctacggcgt gaccatggag gagcccgggc tctcgcgcct
     9781 cgtctcagtc aaactgaccg aagcctgacc tgcgctacac tactgcgcat gccgcaagct
     9841 gaagccccgg gcatgtccct cggcgcgtat ctccgttccc ttcgcgaggc caagggcggc
     9901 tccctcgagg acatggcgcg ctcgacccgc gtggggatcc gccatctgga ggccctggag
     9961 gacgagcggc tcgccgacct tccgtcgccc gtcttcgttc gcggcttcat ccgcgcctac
    10021 tgcagcttcc tccgcgagaa cccggagccc gccctcggcc gctatcagga gctcgccggg
    10081 acccgagcgg cggagcaggc cgcgatcgcg ccaccacggc cccgaaacac gtgggcgagc
    10141 agctcggtgg tggtcggcct cgtgctcctg ctgatcctgg gcgtcggtct gatcctggtc
    10201 aacctcagtg tcaagaagaa cggtgga
//
LOCUS       DATMCV010000050         5724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_535357, whole genome shotgun sequence.
ACCESSION   DATMCV010000050 DATMCV010000000
VERSION     DATMCV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535357"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VNU03_01290"
     CDS             complement(<1..1252)
                     /locus_tag="VNU03_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HWP73108.1"
                     /translation="MLRASAVAERAGVPTASLVCEGFRGQAATTATGLGLPGLPTALV
                     PGHVDVQSVDELRQNVASVTVDAVIRNLTEAPARVGAAVVEPAPGDVVFEGTLDEVNR
                     FFYENDWSDGLPIVPPTPDRVAEFLRFCDRPGETSLGVLLLDRRRATIWNVAVNGVMA
                     GCRPEYMPILVALVEAMADPRYGVEHSGNTPGAETLIILNGPLIKELGFNYEQGALRD
                     GVQANTTIGRFWRLYLRNIAGFLHHRTDKGTFGNTWRVVLAENEDALRRIGWSTIAED
                     EGVLAANAVTISRYTGGGVIASVFGNTPQLMLPYLADAIVAKVGWELLFTVGMGAGSQ
                     RPLLILSPILAETLAKGGLDKAQLKERLFELARVPAGKLERYVGEWTNLMPGQPTLTG
                     LVKAGLIPAVFAESDDPERLVPIVC"
     gene            complement(1271..1594)
                     /locus_tag="VNU03_01295"
     CDS             complement(1271..1594)
                     /locus_tag="VNU03_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73109.1"
                     /translation="MTGSDGREIYRVVWPRGAGTVKAADVAPRLSTLEGKTIGQLWDD
                     LFRGDEIFPILEEELARRFPSVRFVRYDTFGSTHGRDEQRVLAELPARLKELNVDAVI
                     SGMAC"
     gene            complement(1591..2196)
                     /locus_tag="VNU03_01300"
     CDS             complement(1591..2196)
                     /locus_tag="VNU03_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73110.1"
                     /translation="MESAILRIEQDPLARRIAVGARPVLACAARQAGAAIARSARARG
                     RGTAEAALRAEGVVIHESGEEPAAGPFVHHALYTAPPPRVTLFVRTLDALDRHLDATG
                     LRSRIGGVGVREIVLAHELFHHLAATTVMPAAVRPEVDIMQIGPWRRCAVVRAAEEIA
                     AAGFAASWCGVDEAPALLDAATVEIQAVLCSAASRTGRRTE"
     gene            complement(2211..4370)
                     /locus_tag="VNU03_01305"
     CDS             complement(2211..4370)
                     /locus_tag="VNU03_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP73111.1"
                     /translation="MGRLVRIGIDVGGTFTKAVLLDNDSHEIIGQVVVPTTHGAETGV
                     AEGVIDAFRRALGAAGITAADVVFIAHSTTQATNALLEGDVARVGVIGMGRGAVESRL
                     ARSQTVIGDIELAAGRVISSTHTFLRSDEAGADAVRAAVAAVRTQGARVVVASDAFSV
                     DDPRAEEQVMAAAVEAGLPATGGHEISKLYGLTIRTRTAVINASILPKMIETAQLTHG
                     AVRQAGITAPLMIMRGDGGVMDLGEVQKRPVLTMLSGPAASVAGALMYLRVSDGIFFE
                     VGGTSTNVGVIRGGQPTIAFAEVGGHRTYISSLDVRVLGIAGGSMVRLEGDRIVDVGP
                     RSAHIAGLPYAAFQLAASIVDPVVELIQPKADDPAGYVAIRLGDGRRVAITTTDAANV
                     LGRVKAGDYSHGDPEAARRAMAPLAARVGLSLEDTARRILDVAVGKIIPTIERLIEEY
                     GLEREQITLVGGGGGASALIPHAAARLGLEYRIARNAEVISSIGVALAMVRDSVERVM
                     PNPKPEDLLAIRAEAREAAIRAGAHPGTVQVHIEVNPQTQRVRATAWGATEMRARDLL
                     TTLGEDEARAIAATSLKAEPAAVRLAGQTSGHYVFSVDVVEKSFWVFRSTRHPVRVVD
                     RGGFIKVQRADALVSQTSAAGALDELRRLWGLATGYDGNGVLPPDAYLVLGSQVVDLS
                     GMMALDHALSVASSELSGRGPEEPLVLVAAKRVQARL"
     gene            complement(4383..5675)
                     /locus_tag="VNU03_01310"
     CDS             complement(4383..5675)
                     /locus_tag="VNU03_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73112.1"
                     /translation="MSVFLGVVMMLAFLIAVFVIFRGVSPIIVLLLLAIAWPLLAGSS
                     FWDLLTNVLQKGGEQYASTIVIIVFGAWFGQILVQTGIAESMIRTAVELAGDRPIAVA
                     WAVSLITALLFTSIYGVGAAISIGVIAIPIMLSLGIPPKVAAPAFTMAIGAGNYLNPV
                     EFGIFKTFFKGIEYGPPYFTFYLIGFAVYLACALAMSTWNLRGVQVRRASAVGAVAAP
                     PRVRVPWISYLAPAIPVVVIIAFKWPLIPAFLLAIVFALATTQGGRSLRDSVAVFHKA
                     FYDAFPDIATIAALWIICGMLIVAGQLDAVKAVLNPVFSPFIPHSPFGAAVFFALLAP
                     LAIYRGPFSVVGTGAALLAVMLNANYVSPIFLYLCWRGPLCLQGSQDPTNSWTLWTIG
                     YTKISHAEFLKTALPWGWLMVAINAFIAYFMFGSMFTK"
BASE COUNT          863 a         2090 c         2002 g          769 t
ORIGIN      
        1 ggcagacgat cggcaccaaa cgctccggat cgtcggactc ggcgaacacc gcggggatca
       61 gccccgcctt cacgaggccg gtcagcgtgg gctgtccggg catcaggttg gtccactcgc
      121 ccacgtagcg ctcgagcttg cccgccggca cccgcgccag ctcgaagagc cgctccttga
      181 gctgcgcctt gtcgagcccg cccttggcca gcgtctcggc caggatcggg ctcaggatca
      241 ggagcggccg ctggctgccg gcgcccatcc ccaccgtgaa cagcagctcc cagccgacct
      301 tggccacgat cgcgtcggcc aggtacggca gcatcagctg cggcgtgttg ccgaacaccg
      361 aggcgatgac gccgccgccg gtgtagcgcg agatggtgac cgcgttggcg gccagcaccc
      421 cctcgtcctc cgcgatcgtg ctccagccga tccggcgcag cgcgtcctcg ttctcggcca
      481 gcaccacgcg ccacgtgttg ccgaaggtgc ccttgtcggt gcggtgatgg aggaagccgg
      541 caatgtttcg tagatacagt ctccaaaaac ggccgatggt ggtgttggcc tggacgccgt
      601 cgcgcagcgc gccctgctcg tagttgaagc ccagctcctt gatgagcggc ccgttcagga
      661 tgatgagggt ctcggcgccg ggcgtgttgc cgctgtgctc gacgccgtag cgcggatcgg
      721 ccatggcctc gaccagcgcg accaggatcg gcatgtactc ggggcggcag cccgccatca
      781 ccccgttcac cgccacgttc cagatggtgg cgcggcgccg gtcgagcagg agcacgccga
      841 gcgacgtctc gccgggccga tcgcagaagc ggagaaactc ggccacccgg tccggggtcg
      901 gcggcacgat gggcaggccg tcgctccagt cgttctcgta gaagaagcgg ttgacctcgt
      961 cgagcgtgcc ctcgaagacc acgtcgccag gcgcgggctc gaccaccgcg gcgccgacgc
     1021 gcgcgggcgc ctcggtgagg ttccggatca ccgcgtcgac cgtcaccgag gccacgttct
     1081 gccgcagctc gtcgacgctc tgcacgtcga cgtggccggg cacgagcgcc gtcggcaggc
     1141 cgggcaggcc gagcccggtg gcggtggtgg ccgcctggcc ccggaagccc tcgcacacga
     1201 gcgacgcggt cggcaccccc gcccgctccg ccaccgcact ggcccgcaac acggcgggcg
     1261 tgcagctccc tcagcaggcc atccccgaga tgaccgcgtc gacgttcagc tccttcagcc
     1321 gggcgggcag ctcggccagc acccgctgct cgtcgcggcc gtgggtggag ccgaaggtgt
     1381 cgtagcgcac gaagcggacg ctcgggaagc ggcgcgccag ctcctcctcg aggatcggga
     1441 agatctcgtc gccccggaag agatcgtccc agagctgccc gatggtcttg ccctcgaggg
     1501 tggagagccg cggggcgacg tcggccgcct tcacggtacc ggccccgcgg ggccacacca
     1561 cccggtagat ctcgcgtccg tccgatccgg tcattcggtc cgcctccctg tcctggatgc
     1621 cgccgagcat agcaccgcct ggatctcgac cgtggccgcg tcgagcagcg cgggcgcctc
     1681 gtcgacgccg caccaggacg ccgcgaagcc ggcggccgcg atctcctccg cggcccgcac
     1741 caccgcgcat cgccgccacg gcccgatctg catgatgtcg acctcgggcc gcaccgccgc
     1801 cggcatgacc gtcgtggcgg ccagatggtg gaagagctcg tgggccagga cgatctcccg
     1861 caccccgacg ccgccgatcc gcgagcggag accggtggcg tcgagatgtc gatcgagcgc
     1921 gtccagcgtc cggacgaaga gcgtcacgcg cggcgggggc gcggtgtaga gcgcgtggtg
     1981 gacgaagggc ccggccgccg gctcctcgcc gctctcgtgg atgacgacac cttcggccct
     2041 cagcgcggcc tccgcggtcc cccgcccgcg cgctcgcgcc gaccgcgcga tcgccgcgcc
     2101 ggcctgtcgg gccgcgcacg ccaggacggg acgcgccccc accgcgatgc gacgggcgag
     2161 cggatcctgc tcgatccgga ggatcgcgga ctccatccag ccgtacgcga tcacagccgc
     2221 gcctgcactc gcttggccgc cacgagcacc aggggctcct cgggtccgcg tccggaaagc
     2281 tcgctcgagg ccaccgagag cgcgtggtcg agcgccatca tgccggagag atccaccacc
     2341 tggctgccca gcaccaggta ggcgtcgggc ggcagcaccc cgttgccgtc gtaaccggtc
     2401 gccaggcccc acagacgccg gagctcgtcc agcgccccgg ccgcggacgt ctggctcacc
     2461 agcgcgtcgg cgcgctgcac cttgatgaag ccccctcggt cgaccacccg caccggatgc
     2521 cgcgtgctgc gaaagaccca gaagctcttc tccaccacgt cgacgctgaa gacgtagtgg
     2581 ccgctcgtct gaccggcgag ccgcaccgcg gccggctccg ccttcagcga cgtggccgcg
     2641 atcgcgcgcg cctcgtcctc gcccagggtc gtcaggaggt cgcgcgcccg catctcggtg
     2701 gcgccccacg cggtggcgcg cacccgctgg gtctgggggt tcacctcgat gtgcacctgc
     2761 accgtgcccg ggtgcgcgcc ggcgcggatc gccgcctcgc gcgcctccgc gcggatggcc
     2821 agcaggtcct ccggcttcgg gttcggcatc acccgctcga cgctgtcgcg caccatggcg
     2881 agggcgacgc cgatcgagga gatgacctcc gcgttccggg cgatgcggta ctcgagcccg
     2941 agccgggccg ccgcgtgcgg gatgagcgcg gaggccccgc ccccgccgcc caccagcgtg
     3001 atctgctcgc gctcgagccc gtactcctcg atcagccgct cgatggtggg aatgatcttg
     3061 ccgacggcca cgtccaggat gcggcgcgcg gtatcctcca gcgagagccc gacccgcgcg
     3121 gccagcgggg ccatcgcgcg gcgggccgcc tccgggtcgc cgtgggagta gtcgcccgcc
     3181 ttcacccggc cgagcacgtt ggcggcgtcg gtggtggtga tggccacgcg ccggccgtcg
     3241 cccaggcgga ttgccacgta gccggcgggg tcgtccgcct tcggctggat cagctcgacc
     3301 acgggatcga cgatggacgc ggcgagctgg aaggccgcgt agggcagccc cgcgatgtgg
     3361 gcgctgcgag ggcccacgtc cacgatgcga tcgccctcga gccgcaccat gctgccgccc
     3421 gcgatgccga gcacgcggac gtcgagcgag ctgatgtagg tgcggtgccc gcccacctcc
     3481 gcgaacgcga tggtcggctg gcccccgcgg atgaccccga cgttggtgct ggtgccgccc
     3541 acctcgaaga agatgccgtc ggagacgcgc aggtacatga gcgcgccggc cacgctggcc
     3601 gccgggccgg agagcatggt cagcaccggg cgcttctgca cctcgccgag atccatcacc
     3661 ccgccgtccc cgcgcatgat catgagcggc gcggtgatgc cggcctggcg caccgccccg
     3721 tgggtgagct gggccgtctc gatcatcttg ggcaggatgc tcgcgttgat caccgcggtg
     3781 cgggtccgga tggtgagccc gtagagcttg ctgatctcgt gcccgccggt ggcgggcagg
     3841 ccggcctcga ccgcggcggc catcacctgc tcctccgcgc gcgggtcgtc cacgctgaac
     3901 gcgtcgctcg ccaccaccac ccgcgcgccc tgggtccgca ccgcggccac cgcggcccgc
     3961 accgcgtcgg cccccgcctc gtcgctccgc aggaaggtgt gggtggagga gatgacgcgc
     4021 cccgcggcga gctcgatgtc gccgatcacg gtctggctcc gggccaggcg cgactccacc
     4081 gcgccgcgcc ccatcccgat cacccccacc cgcgccacgt cgccctcgag cagcgcgttg
     4141 gtggcctggg tggtggagtg cgcgatgaac accacgtccg cggcggtgat gcccgccgcc
     4201 cccagcgcgc ggcggaaggc gtcgatcacc ccctcggcca cgcccgtctc ggcaccgtgc
     4261 gtcgtgggaa ccaccacctg gccgatgatc tcgtggctgt cgttgtcgag cagcacggcc
     4321 ttggtgaagg tcccgccgac gtcgatgccg atgcggacga gccgtcccac cggttgcgaa
     4381 tcctacttgg tgaacatcga gccgaacatg aagtaggcga tgaacgcgtt gatcgcgacc
     4441 atcagccagc cccacggcag ggccgtcttg aggaactccg cgtgggagat cttggtatag
     4501 ccgatggtcc acagggtcca ggagttggtc gggtcctggc tgccctgcag gcagagcggt
     4561 ccgcgccagc agagatacag gaagatcggg gagacgtagt tggcgttgag catcaccgcg
     4621 agcagcgcgg cgccggtgcc caccacgctg aacggcccgc ggtagatggc gagcggagcc
     4681 agcagcgcga agaagaccgc ggccccgaac gggctgtggg ggatgaacgg tgagaacacc
     4741 gggttcagca ccgccttcac cgcgtcgagc tggccggcga cgatcagcat gccgcagatg
     4801 atccacagcg cggcgatggt cgcgatgtcc gggaaggcgt cgtagaacgc cttgtgaaac
     4861 accgccaccg agtcccggag cgagcgaccg ccctgggtgg tggccagcgc gaacacgatc
     4921 gcgagcagga aggccgggat cagcggccac ttgaacgcga tgatgaccac gaccgggatc
     4981 gcgggcgcca ggtacgagat ccacggcacc cgcacccgcg gtggcgcggc caccgccccc
     5041 accgcggagg cccgacgcac ctggacgcct cgcaggttcc aggtcgacat cgcgagcgcg
     5101 cacgccaggt agaccgcgaa gccgatcagg tagaacgtga agtagggcgg cccgtactcg
     5161 atgcccttga agaaggtctt gaagatgccg aactcgacgg gattcaggta gttgcccgcc
     5221 ccgatggcca tggtgaacgc gggggccgcg accttcggcg ggatgccgag cgagagcatg
     5281 atcgggatcg cgatgacccc gatcgagatc gcggcgccga ccccgtagat cgaggtgaag
     5341 agcagcgcgg tgatgagcga gaccgcccac gccaccgcga tgggtcggtc gccggccagc
     5401 tcgaccgcgg tgcggatcat gctctccgcg atgccggtct gcaccaggat ctggccgaac
     5461 caggccccga acacgatgat cacgatggtg ctcgcgtact gctcaccgcc cttctggagg
     5521 acgttggtca ggaggtccca gaaagacgag ccggccagca gcggccacgc gatggccagc
     5581 aggagcagca cgatgatggg gctcacgccc cggaagatga cgaagaccgc gatgaggaag
     5641 gccagcatca tgacgacgcc caggaacacg ctcatggtcg cctcctagtt gaggccgagc
     5701 accgcacgga cggcctcgcc cttc
//
LOCUS       DATMCV010000051         6097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_602092, whole genome shotgun sequence.
ACCESSION   DATMCV010000051 DATMCV010000000
VERSION     DATMCV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602092"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="VNU03_01315"
     CDS             complement(<1..1186)
                     /locus_tag="VNU03_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP73113.1"
                     /translation="MAEHAGVVRRREDPRLITGAGEFVDDLRVPGCLHAAMLRTPHAH
                     ARIRSIDVAAARRAPGVVGVFVAGDLGAVGDPMPIYAPHPALPVPCKIRPLASDRVRF
                     VGEAVAVVIADDVYRAYDALDLIRVEYEPLPAVVDVEAALAPGAPILHEEIGGNVVAE
                     WRQRVGDAEAALRAAPVVVRTRIRLARGGAHPLEPRSLVAEWRDGLLTIRGAVQMVHR
                     HRGVIAGQLGLREDQVRVIAPPDVGGGFGTKGMYYPEYIVVAELARRLDRPIKWVETR
                     REHTLVACVERDQVHDVEIAATRDGALLALKDDFLHEMGAYTISGLNLPQNAMIHSVG
                     SYVIPALDLRFRGVMTTTTPVGAYRGAGRPYGTGVIERAMDALARELRLDPIELRRRN
                     LVP"
     gene            complement(1285..2799)
                     /locus_tag="VNU03_01320"
     CDS             complement(1285..2799)
                     /locus_tag="VNU03_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HWP73114.1"
                     /translation="MLETLQNLGLGFQVALAPGVLWYGFIGCLVGTLVGVLPGVGPLA
                     GISLLLPATYGLDATSALVMLAGIYYGAMYGGSTTSILMRIPGEAASVMTCIDGYAMA
                     RKGRAGPALAIAAVGSYVAGTVSVIALMFLAPPLAAFALRFGPPEYFSLLLLGLLVLA
                     YMNSGSMLKGLAMAAAGLLLGMIGIDQMSGYFRFAYGVAELGDGIGVVPVAVGLFGLA
                     EILLTAGQPNPPEVIRPRLRDLLPSRQEWREANMPIWRGTAIGFLIGIIPGSAHVIST
                     FVSYAVERKVSKHPEEFGRGAVAGVAGPESANNAATSGAFVPMLALGVPGVPVAALLL
                     AAMMVHGVTPGPLLIQQDPRLFWGFIASMYVGNVILLILNLPLVGLFVNLLRVPYPVM
                     YPIILVCSILGVYAVNSSVVDVWIMLGTGVLGYVLRKLDFETAPIVLGLVLAPMMELS
                     LRQALAMSAGNYTIFVTRPISLTLLTVGVVLVVLALRPLISRTMDWRSRLPTET"
     gene            complement(2818..3258)
                     /locus_tag="VNU03_01325"
     CDS             complement(2818..3258)
                     /locus_tag="VNU03_01325"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HWP73115.1"
                     /translation="MTVDRVAGVALVLIGLATLWESRAFPLGSLHRPGPAYMPVVLAL
                     LLIVFGVAVFAMGRGARTLREVGWPEWRHAVAIFAACAFAAWGLERLGYRLTMAVVVG
                     FLLLALERKGWVLGLGLTLVMAWGSFYLFDTLLRVPLPRGPFGM"
     gene            complement(3272..4255)
                     /locus_tag="VNU03_01330"
     CDS             complement(3272..4255)
                     /locus_tag="VNU03_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWP73116.1"
                     /translation="MLRALAGTCLIVALGTLTVAGGPAWAEDPYPTRPVSMVVAFPPG
                     GLADNTARPVATALERILKQPVAIINKAGAAGAVGNQTVATSKPDGYTLLMALVSISV
                     LPEVDKLFGRPQNYTREQFTGIARINADPSMLVVRADAPWKTLKDFVDDAKKRPGEIV
                     FSSSGLYGTAHIPMEMLTLAAGIKLRHLPTTGGGPMMNAILGGHAQAVMTPVSLAAAH
                     VKAGKLRLLAHSGTGPVAAYPEVPTFKAQGYDVEYTAWAGLVAPRATPPHIVKILRDA
                     TRQAVQSPEVVTSHAKLETPIAYMDADEFNAWWAKDAARLAEVVKQIGRVE"
     gene            5132..5968
                     /locus_tag="VNU03_01335"
     CDS             5132..5968
                     /locus_tag="VNU03_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWP73117.1"
                     /translation="MRLHRTDARRLTAEAELRGINAELEARVQARTLALVASEARYRQ
                     LIEESPDGIMIHQAGVIRLMNRAGLQMFGHAEPAQAVGRPLLDYIAPEHRNIVAARVA
                     SRLAQRDSTTQVIEVETLRTDGTRFWIGAVATVIDWEGSPASLVSFVDISERRRREVV
                     EREAESLRAVAKLANAAAHEINNPLTVVGGNMHLLAARIADRPELQRYVDRVLRAVQT
                     IADMINHMTRITRLSALSHLDTAGVPILDLRSSSQAPEPVPGPAPGPEPDRAPGEPPR
                     PL"
BASE COUNT          893 a         2253 c         2169 g          782 t
ORIGIN      
        1 ccggcaccag gttgcggcgg cgcagctcga tcggatccag gcgcagctcg cgcgcgagcg
       61 cgtccatcgc ccgctcgatc accccggtgc cgtacggccg gccggcgccg cggtaggcgc
      121 ccaccggcgt ggtggtcgtc atcacgccgc ggaagcgcag gtcgagcgcc gggatcacgt
      181 aggagccgac cgagtggatc atcgcgttct gcggcaggtt gaggccggag atggtgtagg
      241 cccccatctc gtggaggaag tcgtccttga gggcgagcag ggcgccgtcg cgggtggccg
      301 cgatctccac gtcgtgcacc tggtcgcgct ccacgcaggc cacaagcgtg tgctcgcgac
      361 gcgtctcgac ccacttgatc ggccggtcga gacggcgggc gagctcggcc accacgatgt
      421 actcggggta gtacatgccc ttggtgccga agccgccgcc gacgtcgggc ggcgcgatga
      481 cgcgcacctg atcctcgcgg agcccgagct ggcccgcgat caccccgcgg tgccggtgga
      541 ccatctgcac cgcgccgcgg atcgtcagca ggccgtcgcg ccactcggcg acgagcgagc
      601 gcggctcgag cgggtgcgcg ccgccgcggg ccagccggat gcgggtgcgc accaccaccg
      661 gcgcggcgcg cagcgcggcc tccgcgtcgc ccacgcgctg gcgccactcc gccaccacgt
      721 tgccgccgat ctcctcgtgc aggatgggcg cgcccggggc cagcgcggcc tccacgtcga
      781 ccaccgcggg cagcggctcg tactcgacgc ggatcaggtc gagcgcgtcg tacgcgcggt
      841 agacgtcgtc cgcaatcacc accgccaccg cctcgcccac gaagcgcacc cggtcgctcg
      901 cgagcgggcg gatcttgcag ggcaccggca gcgcgggatg cggggcgtag atcggcatcg
      961 ggtcgcccac cgcgcccagg tcgcccgcca cgaagacgcc caccacgccg ggcgcacggc
     1021 gcgccgccgc cacgtcgatg ctccgaatgc gcgcgtgcgc gtgcggcgtg cgcagcatcg
     1081 cggcgtgcag gcaccccggc acccgcagat cgtccacgaa ctcccccgcc ccggtgatca
     1141 gccgcggatc ctccctccgc cgcaccaccc ccgcgtgctc agccatcgcg tcgtccgggg
     1201 tcaggtctta cattccgaca tttccacccc gtctcggctc gtcggggcga gagaaaatgt
     1261 cggaatgtaa gacctgaccc caatctaggt ctccgtgggg aggcgggagc gccagtccat
     1321 cgtccgggag atgagcgggc ggagggccag taccacgagc acgacgccca ccgtcaggag
     1381 cgtgagcgag atggggcggg tgacgaagat cgtgtagttc cccgccgaca tggccagcgc
     1441 ttgccgcaaa gacagctcca tcatcggggc caggaccagg cccagcacga tgggcgcggt
     1501 ctcgaagtcg agcttgcgga gcacgtagcc gagcacgccg gtgcccagca tgatccacac
     1561 gtcgaccacg ctgctgttga ccgcgtagac cccgaggatc gagcacacga ggatgatcgg
     1621 gtacatcacc gggtacggaa cccgcagcag gttcacgaag aggccgacca ggggcaggtt
     1681 gaggatcagc aggatcacgt tgcccacgta catcgaggcg atgaagcccc agaagagtcg
     1741 cgggtcctgc tggatcagca gcggccccgg cgtgaccccg tgcaccatca tcgcggccag
     1801 cagcagcgcg gcgaccggca cccccggcac gcccagggcc agcatcggca cgaaggcccc
     1861 cgacgtcgcc gcgttgttgg ccgactcggg cccggccaca cccgccaccg cgccgcgtcc
     1921 gaactcctcc gggtgcttcg acaccttgcg ctcgaccgcg tacgacacga aggtcgagat
     1981 gacgtgcgcg gagcccggga tgatgccgat caggaagccg atcgcggtgc cgcgccagat
     2041 cggcatgttg gcctcgcgcc actcctgccg cgagggcagc aggtcgcgca ggcgcgggcg
     2101 gatgacctcc ggcgggttgg gctgcccggc ggtcaggagg atctcggcga ggccgaagag
     2161 tcccaccgcc accggcacca ccccgatgcc gtcgcccagc tccgccaccc cgtaggcgaa
     2221 gcggaagtag ccgctcatct ggtcgatgcc gatcatgccg aggagcagcc cggccgcggc
     2281 catggccagg cccttcagca tcgagcccga gttcatgtag gcgagcacca gcaggccaag
     2341 gagcaggagc gagaagtact cgggcgggcc gaagcgcagc gcgaaggcgg cgagcggcgg
     2401 ggccaggaac atgagcgcga tgacgctcac ggtgcccgcc acgtacgagc ccaccgcggc
     2461 gatggccagc gccggccccg cccgcccctt gcgcgccatc gcgtagccgt cgatgcacgt
     2521 catcaccgag gcagcctcgc ccgggatgcg catcaggatc gaggtggtgg agccgccgta
     2581 catcgccccg tagtagatgc cggccagcat caccagcgcg ctggtggcgt cgaggccgta
     2641 ggtcgcgggc agcagcaggc tgatgccggc gagcgggccc acgccgggca gcacgcccac
     2701 cagcgtgccg accaggcagc cgatgaaccc gtaccagagg acgccgggcg cgagcgccac
     2761 ctggaagccc aggcccaggt tctggagggt ctcgagcatc agctcacaga ccgcccatca
     2821 cataccgaaa ggcccgcgcg gcagcggcac ccgcagcagc gtgtcgaaga ggtagaacga
     2881 gccccacgcc atcacgagag tgagcccgag ccccagcacc cagcccttgc gctcgagcgc
     2941 cagcagcagg aagcccacca ccaccgccat ggtgagccgg tagccgagcc gctcgagccc
     3001 ccacgcggcg aaggcgcacg ccgcgaagat ggccaccgcg tggcgccact cgggccaccc
     3061 cacctcgcgg agcgtgcggg ccccgcggcc catggcgaag acggccacgc cgaagacgat
     3121 cagcagcagc gccagcacca ccggcatgta cgcggggccc gggcgatgga gcgagccgag
     3181 cgggaaggcc cggctctccc agagggtcgc caggccgatg aggacgagcg ccacgcccgc
     3241 gacgcggtcg acggtcatgg cgcgggaggg atcactcgac tcggccgatc tgcttcacca
     3301 cctcggccag ccgcgccgcg tccttcgccc accacgcgtt gaactcgtcc gcgtccatgt
     3361 aggcgatggg cgtctcgagc ttggcgtgcg acgtcaccac ctcgggcgac tgcaccgcct
     3421 gccgggtggc gtcgcgcagg atcttgacga tgtgcggcgg ggtggcccgg ggcgccacca
     3481 gaccggccca cgcggtgtac tccacgtcgt agccctgcgc cttgaaggtc ggcacctccg
     3541 gatacgccgc caccggcccg gtgccgctgt gggccagcag ccgcagcttg cccgccttca
     3601 cgtgggcggc ggccaggctc accggcgtca tcaccgcctg cgcgtgcccg cccaggatgg
     3661 cgttcatcat cggcccgccc ccggtggtgg gcaggtggcg gagcttgatc ccggccgcca
     3721 gcgtgagcat ctccatcggg atgtgcgcgg tgccgtagag gccggaggag ctgaagacga
     3781 tctcgccggg ccgcttcttg gcgtcgtcca cgaagtcctt cagcgtcttc cagggcgcgt
     3841 cggcgcgaac cactaacatg gaagggtcgg cgttgatgcg ggcgatgccg gtgaactgct
     3901 cgcgcgtgta gttctgcggg cggccgaaga gcttgtcgac ctcgggcagc accgagatgc
     3961 tcaccagcgc catcagcagc gtgtagccgt cgggcttgga ggtcgccacc gtctggttgc
     4021 cgaccgctcc cgccgcgccc gccttgttga tgatggccac cggctgcttg aggatgcgct
     4081 cgagcgcggt cgccaccggc cgcgcggtgt tgtcggcgag gccgccgggc gggaaggcca
     4141 ccaccatgga gacggggcgc gtgggatacg gatcctcggc ccacgcgggg ccgccggcca
     4201 cggtcagcgt gcccagcgcg acgatcaggc aggtcccggc gagcgctcgg agcatgcaca
     4261 tctcctttga tgggggtgtc gacggtggtg cggccagggc ggaagcctag cagccgctcg
     4321 ggcggcgggt caaggctccc ggccgctcgg gagggctccg ggtgctagga tcgatggcag
     4381 cccgcgatga ccccctcgcc cctcgtcctt ctacacggcc tgtgtaccgg ggccgcggcg
     4441 ctctgcgcac tcgtgggcat cgcggtgctg gtcggcgact gggctctcgg cgagcccctg
     4501 gcgctgatga agccctccac cgcggcgtgc ttcgcgctgg ccggcgcctc cctgtgggta
     4561 ttgagcgagc ggcccgcggc ccgccgggtg agggtcctgg ccgacggcgc cgccgggatc
     4621 gtgggcctgc tcgcggtcat cacgctcacc gcgcagctcg caggacggga tccggcgggg
     4681 cgcatgacct cgccgaccgc gctgtgcttc ctgctgctgg ccctcgccct gctcagcctg
     4741 gaccgcgtcc cggtcttccc gaccggccag cggctcgcgg tggtctccgg cttcctctcc
     4801 ctgctcgtcg tggtcgcgtg ggcgtacggc gccgggacgc cgccgcgcgg cccggcggcg
     4861 atggcgcccc tcaccgggat cctcctcgtc atcctgagcg tcgcggtgac cgcggcccgc
     4921 ccgggccgcg gcgccaccgg catcttcgtc caggacaccg cgggcggcat ggtggcccgc
     4981 tgggtgttcc cggggaccac gctcggggcg ctcgtggtgg gggcgctcgc gctggcgggc
     5041 gagcgcgccg gcctctacgg ccgcgagttc gccgtcgcct tcacgacagt ggcgagcctc
     5101 tctcttctcg gccctggtct ggttcatctc ggtgcgcctg caccgcacgg acgcgcgccg
     5161 cctgaccgcc gaggccgagc tgcgtgggat caacgcggag ctcgaggcgc gggtgcaggc
     5221 gcgcacgctg gccctcgtcg cgtcggaggc gcggtatcgc cagctgatcg aggagtcccc
     5281 cgacggcatc atgatccacc aggcgggcgt gatccggctc atgaaccggg cgggcctgca
     5341 gatgttcggc cacgccgagc cggcgcaggc ggtgggacgg cccctcctcg actacatcgc
     5401 ccccgagcac cggaacatcg tggccgcgcg ggtagcctcg cggctggcgc agcgcgattc
     5461 gacgacgcag gtgatcgagg tcgagacgct ccggacggac ggcacccgct tctggatcgg
     5521 cgcggtcgcc accgtcatcg actgggaggg cagcccggcc agcctcgtgt cgttcgtgga
     5581 catcagcgag cggcgccggc gcgaggtggt cgagcgggag gcggagagcc tccgcgcggt
     5641 ggccaagctg gccaacgcgg cggcgcacga gatcaacaac cccctcaccg tggtcggcgg
     5701 caacatgcac ctgctcgccg cgcggatcgc cgatcgcccc gagctccagc gctacgtcga
     5761 tcgggtcctg cgcgcggtgc agaccatcgc ggacatgatc aaccacatga cgcgtatcac
     5821 ccggctctcg gcgctctcgc acctcgacac cgccggcgtg ccgatcctcg acctgcgcag
     5881 ctcgagccag gcccccgagc ctgttcccgg ccccgccccc ggccccgagc ccgaccgcgc
     5941 ccccggagag cccccgcgac cgttgtagac taccggccat gagcgcgagc ggtgggagca
     6001 tcgacaccgg caccggctac ggccgacccc gcccgaccac gatgcgcgag ctgaccgagg
     6061 tgggcgccgg caccccgatg ggcgagctgc tgcgccg
//
LOCUS       DATMCV010000052         6613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_402724, whole genome shotgun sequence.
ACCESSION   DATMCV010000052 DATMCV010000000
VERSION     DATMCV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402724"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..547
                     /locus_tag="VNU03_01340"
     CDS             146..547
                     /locus_tag="VNU03_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP73118.1"
                     /translation="MQAVHADDREWETLRWPGQESKMLFHPSPDCPTAPNAGLVRYEP
                     GSHHPFHKHDFAQVWYILEGEFQIGETLHGPGSMLFYPDPHFEAPLSTRTGGLMLFVQ
                     YQGPTTGGRPIYDGRFNMTERRAIAEENVER"
     gene            complement(617..2647)
                     /locus_tag="VNU03_01345"
     CDS             complement(617..2647)
                     /locus_tag="VNU03_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP73119.1"
                     /translation="MATTEHASVCPLDCPDTCSLSVTVENAKIVKIRGSRANPYTAGV
                     ICTKVAGSYPDWVHGPGRLQTPLRRIGAKGEGRFARASWDEALDIIHDRVTAVIAAHG
                     PQAVAPFNYAGPHGMLAYASMDLRFFHKLGASQLDRSPLCGGIRTQAWLGTFGTAPGI
                     APEQVRHSKLIIAWGNNVTWSNLHLTPLLNAARRDGAKVVVVDPKRIKVAEQSDLHIA
                     LKPGTDVVLAWAVAAELERRGAFDRAFIERHVEGFEEFMAGARRYSVADAARICGVPE
                     AQIRQLAEWYHTLSPAAISVGNGLERNQNGGSGIRAIFALPALAGKFGVPGGGLVNGA
                     GFVFPKTPAKLQRPDLLPPGTRTLNIVDMGKHLLDPSLSPPLKALFVYNHNPLVVHPD
                     QNRMRRALAREDLFTVGCDVVMTDSLAYADVVLPASSHFEYHDIYAAYGTHWLQRAEP
                     VIEPQHESLPNTEIFRRLAARFGFTDPVFTASDAQLMDDAVDGSDPRLGGVAPSRLPT
                     DRATAMTVGGEPAILYKNVFPKTPSGKVELASGYLEQKYGSRLPTYRAYETPFPLLLI
                     SPASDERITSTFGGLKPGDVTPPLEMHPDDAKARGLRDGVTVRVWNELGEVHLPLQVS
                     DAIRPGVVCSLKGAWMRTSDNGQTVSALAPAHHADICGGACYNDCRVEVAAL"
     gene            2774..3151
                     /locus_tag="VNU03_01350"
     CDS             2774..3151
                     /locus_tag="VNU03_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73120.1"
                     /translation="MKKLIALVLAVAVFAPTLAFAGASTDAALGLGAFAVFNQILGGV
                     GVFHRGPVLAPAPVVVARPAPVYVPAPIVVAPPRVIARPYPIYVPVPRHVVVHERPVF
                     CPPGHARKGWCYPAPVVHPGWRY"
     gene            3281..3859
                     /locus_tag="VNU03_01355"
     CDS             3281..3859
                     /locus_tag="VNU03_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWP73121.1"
                     /translation="MRRVSLLRLIVVFAWTGLSSLGGGRSAYFHESLVRRRGWIRHDE
                     FVQDFTLSQVLPGPNFSNLAVVLGYRLAGFAGGAWGLLAVVLPGALILLVASGLYAEG
                     AFGAGATLLMRGMSAAVVGLVAVTSLRIMRGSLRGARAAAIALLTFVAVGPLRLPTVA
                     VVLVMTALSFGLYRAAPGPQPALPPTPPVENA"
     gene            3859..4380
                     /locus_tag="VNU03_01360"
     CDS             3859..4380
                     /locus_tag="VNU03_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWP73122.1"
                     /translation="MANLAVLTWTFLWLSFLCVGGGLGVIPEMERQAVTIHHWLTARE
                     FVDGYTLSQLTPGPNMLVAVFVGHRVHGLLGAALAGLAMFVPTSILAIVISHHWRQWR
                     DRPWAIAAERALLPVGVGLMAGGVYTLARTAIHDGVTATLAGATAIVVATDRVPPVVA
                     ILAAGVIGWLLWR"
     gene            4610..5677
                     /locus_tag="VNU03_01365"
     CDS             4610..5677
                     /locus_tag="VNU03_01365"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWP73123.1"
                     /translation="MLSPVEHAPLAPHCTLRIGGPARYLVEATDEATVAAAVEWASAR
                     RLPVRVLGGGSNLVIADEGVDGLVLKITLRGVSTRDLPGAPGAIEVTAAAGEPWDGLV
                     RHVVERGWAGLECLSGIPGLVGATPIQNVGAYGQEVSDTLTAVRALDRESGRVVTLGP
                     AECGFGYRDSLFKRGAPGRHVVLAVSYRLTPGGPPDVRYADIARDLEARGLARPSLAE
                     VRETVLKIRRSKSMVLDPGDPNARSCGSFFLNPIVPADALARIDARAAGLSMPRWPLP
                     DGHVKLSAAWLIERAGFTRGQADGPVGLSTRHTLAIVCHDGARARDVAAFARRVRAGV
                     EQRFGVRLHPEPIFWGSLSLD"
     gene            5771..>6613
                     /locus_tag="VNU03_01370"
     CDS             5771..>6613
                     /locus_tag="VNU03_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWP73124.1"
                     /translation="MKITAIRATWLRAPIPAEHAHVSDFGRNDTFNMCLVEIETDTGL
                     TGLGEAKAAVGNLGNYAAMVTLIREEMAPVLIGRDPRDIAGIWETLYNGSRAHYATRE
                     GRTFPTIGRRGLALCAISGIDIACWDLLGRSLGQPVWRLLGGKYRDRIPAYASGGWAP
                     VGAIGKQMTQYVERGHRAVKLRVGLQDKSVDDSAARVREVRETLGPDVGIMVDAHGTW
                     SVRQAQRFARKVEDCDLGWLEEPVSAENLAGQAEVRASTDIPIAAGESEQTRFAFRDM
                     IEARA"
BASE COUNT          857 a         2370 c         2397 g          989 t
ORIGIN      
        1 gctacacgcc gcgcgggcca gaggacgtcg tcacgcccgc gcggcgcgac ggctggatcg
       61 ggcgctgcct cgccaccgag ctcgggcgcg tgaacgccag aaagtgagcg gagaccccat
      121 gtcgcaggat ctgaagcatc agccaatgca agcagtgcat gccgacgacc gggagtggga
      181 gacgctgcgc tggccaggac aggagagcaa gatgctcttc cacccgagcc ccgactgccc
      241 caccgcgccc aacgcggggc tggtgcgcta cgagccgggc tcgcaccacc ccttccacaa
      301 gcacgacttc gcccaggtct ggtacatcct cgagggtgag ttccagatcg gcgagacgct
      361 ccacgggccg ggctcgatgc tcttctaccc ggatccgcac ttcgaggcgc cgctctccac
      421 ccgcaccggg gggctgatgc tgttcgtgca gtaccagggc ccgacgacgg gcggccggcc
      481 gatctacgac gggcgcttca acatgacgga gcgccgcgcg atcgccgagg agaacgtgga
      541 gaggtagcct ctcaccctgg gcccctcacc ctcgggcccc tcaccctgcc ctctcccctg
      601 aggggagagg gggaggctag agggcggcga cctcgacgcg gcagtcgttg tagcaggcgc
      661 cgccgcagat gtcggcgtgg tgggcggggg ccagcgcgga gacggtctga ccgttgtcgc
      721 tggtgcgcat ccacgcgccc ttcaggctgc agaccacccc gggccggatc gcgtcggaga
      781 cctggagcgg caggtgcacc tcgcccagct cgttccacac ccgcaccgtc accccgtcgc
      841 gcaggccgcg ggccttcgcg tcgtccgggt gcatctcgag cggcggcgtc acgtcgcccg
      901 gcttgagccc gccgaaggtg gaggtgatgc gctcgtcgga ggcgggtgag atgagcagca
      961 gcgggaacgg cgtctcgtag gcgcggtagg tcggcagccg gctcccgtac ttctgctcga
     1021 ggtagcccga ggccagctcc accttgcccg acggcgtctt ggggaagacg ttcttgtaga
     1081 ggatggcggg ctcgccgccc accgtcatgg cggtcgcgcg atcggtcggc agccggctcg
     1141 gagccacgcc gcccagtcgc gggtcggacc cgtccaccgc gtcgtccatg agctgggcgt
     1201 cgctggcggt gaagacgggg tcggtgaagc cgaagcgcgc ggccagcctc cggaagatct
     1261 cggtgttggg cagcgactcg tgctgcggct cgatcaccgg ctccgcgcgc tgcagccagt
     1321 gcgtgccgta agccgcgtag atgtcgtggt actcgaagtg gctcgaggcg ggcagcacca
     1381 cgtccgcgta ggcgaggctg tcggtcatca ccacgtcgca gcccaccgtg aacagatcct
     1441 cgcgcgcgag cgcccgccgc atccggttct ggtccgggtg caccaccagc gggttgtggt
     1501 tatagacgaa gagcgccttg agcggcgggg agaggctcgg gtcgaggaga tgcttgccca
     1561 tgtccacgat gttgagcgtg cgggtgcccg gtgggagcag atccggacgc tgcagcttgg
     1621 ccggcgtctt ggggaacacg aagccggcgc cgttcacgag gccgccgccc ggcaccccga
     1681 acttcccggc cagcgcgggc agggcgaaga tcgcccggat gccgctgccg ccgttctggt
     1741 tgcgctcgag cccgttgccc acgctgatcg cggcgggcga gagcgtgtgg taccactccg
     1801 cgagctgccg gatctgggcc tcgggcacgc cgcagatccg cgccgcgtcc gccaccgagt
     1861 agcggcgcgc gccggccatg aactcctcga agccctccac gtggcgctcg atgaacgcgc
     1921 ggtcgaaggc tccgcgccgt tccagctccg ccgcgacggc ccacgccagc accacgtcgg
     1981 tgccgggctt gagcgcgatg tgcagatccg actgctccgc gaccttgatg cgcttggggt
     2041 cgacgaccac caccttggcg ccgtcgcgcc gggccgcgtt gagcagcggg gtcaggtgga
     2101 ggttcgacca ggtgacgttg ttgccccagg cgatgatgag cttggagtgc cgcacctgct
     2161 cgggcgcgat gccgggcgcg gtcccgaagg tgccgagcca ggcctgggtg cggatgccgc
     2221 cgcagagcgg cgagcggtcc agctgcgagg cgccgagctt gtggaagaag cgcaggtcca
     2281 tcgatgcgta ggcgagcatc ccgtgaggcc ccgcgtagtt gaacggcgcc accgcctgtg
     2341 ggccgtgggc cgcgatcacc gcggtcacgc gatcgtggat gatgtcgagc gcctcgtccc
     2401 acgaggctcg cgcgaaccgg ccctcgccct tggccccgat gcgccgcagc ggagtctgca
     2461 gccgcccggg tccgtgcacc cagtccgggt acgagccggc caccttggtg cagatcacgc
     2521 cggcggtgta ggggttggcc cgggagccgc ggatcttcac gatcttcgcg ttctccacgg
     2581 tgacggacag gctgcaggtg tcggggcagt ccagggggca gacgctcgcg tgctcggtag
     2641 tggccatagg cccgtatcgt aaggcaaacg gggggtttcc gccagcacgt gggacgcgta
     2701 aacaccggga ggtgtccgac cgtctattcc ggcagagggg cggtcgtcat ggcccctcga
     2761 gaggagccca acaatgaaaa agctgatcgc gctggttctg gccgtcgcgg tctttgcccc
     2821 aacgctggcc ttcgcgggtg cgtccaccga cgcggccctc gggctcggcg cgttcgcggt
     2881 cttcaatcag atcctgggcg gagtcggcgt cttccatcgc ggcccggtgc tggcgccggc
     2941 cccggtggtc gtggcgcggc ctgcgcccgt ctacgttccc gcgccgatcg tggtggctcc
     3001 cccgcgcgtg atcgcgcggc cctatccgat ctacgtgccc gtgccgcgac acgtggtggt
     3061 ccacgagcgg ccggtcttct gcccgcccgg tcacgcccgc aagggctggt gctatccggc
     3121 tccggtggtg cacccgggct ggcgctactg atcgcgtcct ttccgaacac ggccggctga
     3181 gcccgaccgg ctgaacacct gccctctccc ccgggccccg ggggagaggg tgctacactc
     3241 caggggccat gccgcccgcg tgctcgctcg agcttgacga gtgaggcgcg tctccctcct
     3301 ccgtctgatc gtggtattcg cctggaccgg cctctcgagc ctgggcggcg gccgcagcgc
     3361 ctacttccac gagtcgctcg tgcggcggcg cggctggatc cgccacgacg agttcgtcca
     3421 ggacttcacg ctgagccagg tgctgccggg accgaacttc agcaacctgg cggtggtgct
     3481 cggctatcgc ctcgccggct tcgcgggcgg cgcgtggggc ctgctcgcgg tcgtcctgcc
     3541 cggcgccctg attctcctcg tcgcgagcgg gctctacgcg gagggagcgt tcggggccgg
     3601 cgccaccctc ctgatgcgcg ggatgagcgc ggccgtggtg gggctcgtgg ccgtcacttc
     3661 gctgcgcatc atgcgcggct cgctgcgggg cgcgcgcgcg gcggcgatcg ccctgctcac
     3721 gttcgtcgcg gtcggcccgc tccgcctgcc caccgtcgcg gtggtgctgg tgatgaccgc
     3781 gctgagcttc ggtctctacc gcgccgcgcc cggtccacag ccggcgctgc cgcccactcc
     3841 gccggtggag aacgcgtgat ggcgaacctg gcagtgctca cgtggacctt cctctggctg
     3901 agcttcctct gcgtcggcgg cggcctcggg gtgatcccgg agatggagcg gcaggcggtg
     3961 accatccacc actggctcac cgcccgcgag ttcgtcgacg gctacacgct ctcgcagctc
     4021 acccccgggc ccaacatgct ggtggcggtc ttcgtgggtc accgggtcca cggcctcctc
     4081 ggcgcggcgc tggccggcct cgcgatgttc gtgcccacct ccatcctcgc catcgtgatc
     4141 tcgcaccact ggcggcaatg gcgcgaccgt ccgtgggcga tcgcggcgga gcgggcgctc
     4201 ctgccggtcg gggtggggtt gatggcgggc ggggtctaca cgctcgcacg caccgcgatc
     4261 cacgacggcg tgaccgcgac cctcgcgggc gccaccgcga tcgtggtggc caccgatcgc
     4321 gtcccgccgg tggtggcgat cctggccgcg ggcgtgatcg gctggctcct gtggcggtag
     4381 cgccgcgccc cggacgcggt cgcgcttgac acgaggtttc gtcccggcat aaggtcgccc
     4441 cggctccggc ggtcgtccta cgaggtggac aacgccaagc gcgtcagaag ttcgcctacc
     4501 agggtctctt ccccatcacc gactagctct cccgagactc tccctctccc ccgtggggag
     4561 agggcagggt gaggggccgg ggccacccgc ggtaagatgg tcccggccca tgctctcccc
     4621 cgtcgagcac gctccgctcg cgccccactg caccctccgc atcggtggcc ccgcccgcta
     4681 cctggtcgag gccaccgacg aggccaccgt cgcggcggcc gtcgagtggg cgagcgcccg
     4741 tcgcctgccg gtgcgcgtgc tcggcggcgg ctcgaacctc gtgatcgccg acgaaggggt
     4801 cgacggtctc gtgctcaaga tcacgctccg cggggtgagc acccgtgacc tgccgggtgc
     4861 gccgggtgcg atcgaggtga ccgcggccgc gggggagccc tgggacgggc tggtgcggca
     4921 cgtcgtcgag cggggctggg ccggcctcga gtgcctgagc ggcatcccgg gcctcgtcgg
     4981 ggccacgccc atccagaacg tgggcgccta cggccaggag gtgagcgaca cgctcaccgc
     5041 ggtgcgcgcg ctcgaccgcg agagcggccg cgtggtcacg ctcggacccg ccgagtgcgg
     5101 gttcggctac cgcgacagcc tcttcaagcg tggggcgccc ggccgccacg tggtgctggc
     5161 ggtgagctac cggctcaccc cgggcggacc tcccgacgtg cgatacgccg acatcgcccg
     5221 cgacctcgag gcgcgcggcc tcgcgcggcc gtcgctggcc gaggtgcgcg agacggtgct
     5281 gaagatccgg cgctccaagt cgatggtgct cgacccgggc gatcccaacg cgcgaagttg
     5341 cggctcgttc ttcctgaatc cgatcgtgcc cgccgacgcg ctggcccgga tcgacgcgcg
     5401 cgcggcgggc ctctccatgc cccgctggcc gctgcccgac ggccacgtga agctctccgc
     5461 ggcgtggctg atcgagcggg ccggcttcac gcgcgggcag gccgatgggc cggtcggcct
     5521 ctccacgcgt cacacgctgg ccatcgtctg ccacgacggc gcccgcgccc gcgacgtcgc
     5581 cgccttcgcg cgccgagtgc gggccggcgt cgagcagcgt ttcggggtgc gcctgcaccc
     5641 cgagcccatc ttctggggta gtctcagcct cgactgacga ccggtcgcgg ggcccggcgc
     5701 tgccgggcct gagcgctccg ccgtccggcg gttgacttcg ctcccgcgcg cgccgtacgc
     5761 tcgccccgcc atgaagatca ccgccatccg agccacctgg ctccgcgcgc ccattcccgc
     5821 cgagcacgcc cacgtgagcg acttcggccg caacgacacc ttcaacatgt gcctggtcga
     5881 gatcgagacc gacaccgggc tcaccggcct cggcgaggcc aaggccgcgg tgggcaatct
     5941 cggcaactac gcggcgatgg tcacgctgat ccgcgaggag atggcgccgg tcctgatcgg
     6001 tcgcgatcct cgggacatcg ccgggatctg ggagacgctc tacaacggct cccgcgccca
     6061 ctacgcgacc cgcgagggcc ggacctttcc caccataggc cggcgcgggc tcgcgctctg
     6121 cgcgatcagc ggcatcgaca tcgcgtgctg ggatctgctc ggccgctcgc tcggccagcc
     6181 ggtgtggcgg ctgctggggg gaaagtaccg cgaccgcatc cccgcctacg cgagcggggg
     6241 ctgggcgccg gtgggcgcca tcggcaagca gatgacccag tacgtggagc ggggtcaccg
     6301 cgcggtgaag ctgcgggtgg ggctccagga caagagcgtg gacgactcgg ccgcgcgggt
     6361 gcgcgaggta cgcgagaccc tggggcccga cgttggcatc atggtcgacg cccacggcac
     6421 gtggagcgtg cggcaggctc agcgcttcgc gcgcaaggtg gaggactgcg atctcggctg
     6481 gctcgaggag ccggtctcgg ccgagaacct ggccggccag gccgaggtgc gcgcctccac
     6541 cgacatcccg atcgcggcgg gcgagagcga gcagacgcgc ttcgcgttcc gcgacatgat
     6601 cgaggcgcgc gcg
//
LOCUS       DATMCV010000053         3356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_735329, whole genome shotgun sequence.
ACCESSION   DATMCV010000053 DATMCV010000000
VERSION     DATMCV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735329"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VNU03_01375"
     CDS             complement(<1..656)
                     /locus_tag="VNU03_01375"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWP73125.1"
                     /translation="MGRQRPSALLQPAQVPAPVDREERPRHHRQESPDRYLSPPVLIL
                     ASASPRRRELLGQLGIPFEIHPSHIPEVHPARPPAEAIAAVALAKALAVAATLGPAAP
                     PAVILGADTEVVLDGRLLGKPRDAADAARMLRDLRGREHQVITGLAIVETGPPGTAPR
                     QETASVTSRVRMGGYGDAEIDAYVATGEPLDKAGGYAVQGLGGRLVEAVDGCFTNVVG
                     L"
     gene            complement(545..1462)
                     /gene="speE"
                     /locus_tag="VNU03_01380"
     CDS             complement(545..1462)
                     /gene="speE"
                     /locus_tag="VNU03_01380"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HWP73126.1"
                     /translation="MATLQYKWFFETTTPVEGHMHAIARTIVTRQTKFQFMEIMDTHS
                     YGKCLVLDGRIQSSQADEFIYHETLVHPGLLAHPRPQRAMVIGGGEGATVREILRYKT
                     ITDCLMVDIDGEVVEECKKHLPEMHQGAFEDPRTRLLHEDARAYLEKTTERFDLIVVD
                     LVEPLEEGPACLLFTKEFYTLIRDRLTEHGVMTMQAGMTKVNEIFFHGAVNRTLKEVF
                     PVVAPYQSFISCFGTPWGFILASKGTDPRKQTPADIDRLIGERLDPEQLGYWDGNAHL
                     HCFSLPKFLRQSIEKNDRVITDKNPLIVT"
     gene            complement(1470..1880)
                     /gene="speD"
                     /locus_tag="VNU03_01385"
     CDS             complement(1470..1880)
                     /gene="speD"
                     /locus_tag="VNU03_01385"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HWP73127.1"
                     /translation="MQALGRHLLLELFDCDAEALNSLDTVKASMVEAAKRAQATIVDV
                     VFHEFNPFGISGVVVIAESHLAIHTWPEYRYAAVDVFSCGDVLQPQVAADYLVEQLGA
                     ARASVVELQRGIFVNHGRPLLHKPAGAPVVTTPG"
     gene            2206..2688
                     /locus_tag="VNU03_01390"
     CDS             2206..2688
                     /locus_tag="VNU03_01390"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HWP73128.1"
                     /translation="MHFGPVTKAAGTAGNAEGSTPLNAFDNALLAAGIGNINLIKVSS
                     ILPPGVELVPLPRIKPGALVPTAYAAMTSEVPGEVVAAAVGWARPNDPAKNGVIMEFH
                     DKATREEAERMIVQMLEEAFRVRGETIRDMQVFAVEHRVERTGCALAAVTLLSDDDLV
                     "
     gene            2698..>3356
                     /locus_tag="VNU03_01395"
     CDS             2698..>3356
                     /locus_tag="VNU03_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HWP73129.1"
                     /translation="MIPAEPRFIACRASLADAGIVLFGIPFEGRVNLRRGADHGPREL
                     RLASDSIETYSPFLGRDLEDLAIADLGDCELGDGTPREQLARAREEIRAFWRPGLRPV
                     MLGGDHTATLAVIEVLAPAIPDLRILQLDAHPDLREEFLGERYNYASAMARVMDVVAP
                     ERVFQVGMRTGAREEYQRKAPHLFPAHRVKPLEAVRSLLPELQAHPLYVTIDVDVLDP
                     S"
BASE COUNT          491 a         1168 c         1160 g          537 t
ORIGIN      
        1 aggcccacca cgttggtgaa acagccgtcg accgcctcca ccagccggcc acccagcccc
       61 tgcaccgcgt agccccccgc cttgtcgagc ggctcgccgg tcgcgacgta ggcgtcgatc
      121 tcggcgtctc cgtagccgcc catccggacc cggctcgtga ccgaggcggt ctcctgtcgc
      181 ggcgccgtgc cgggcgggcc cgtctctacg atcgcgaggc cggtgatcac ctggtgctcg
      241 cgcccccgca ggtcccggag catgcgggcc gcgtcggcgg cgtctcgcgg cttgccgagc
      301 aggcggccgt ccagcaccac ctcggtgtcg gcgcccagga tgaccgctgg cggcgcggcc
      361 ggcccgagcg tcgccgccac cgccagggcc ttggcgaggg cgacggcggc gatggcctcg
      421 gccggcggcc gagcgggatg gacctcgggg atgtgactcg ggtggatctc gaacgggatg
      481 ccgagctgcc ccagcagctc ccggcgccgc ggggaggcgg aggccaggat gaggacgggc
      541 gggctcaggt aacgatcagg ggattcttgt cggtgatgac gcggtcgttc ttctcgatcg
      601 actggcgcag gaacttgggc aggctgaagc agtgcagatg ggcgttgccg tcccagtagc
      661 cgagctgctc gggatcgaga cgctcgccga tgagccggtc gatgtccgcc ggcgtctgct
      721 tccgggggtc ggtgcccttc gaggccagga tgaagcccca cggcgtcccg aagcaggaga
      781 tgaagctctg gtagggggcc accaccggga agacttcctt gagggtccgg ttgaccgccc
      841 cgtggaagaa gatctcgttc accttggtca tgccggcctg catggtcatg accccgtgct
      901 cggtcagccg gtcacggatc agggtgtaga actccttggt gaagagcagg cacgccggcc
      961 cttcctcgag gggctccacc aggtcgacca cgatcaggtc gaagcgctcg gtggtcttct
     1021 ccagataggc ccgggcgtcc tcgtgaagga gccgggtccg ggggtcctcg aaggccccct
     1081 gatgcatctc gggcaggtgc ttcttacact cctcgaccac ctcgccgtcg atgtccacca
     1141 tcaggcagtc ggtgatcgtc ttgtagcgga ggatctcccg gacggtggcg ccctcgcccc
     1201 cgccgatgac catggcccgc tggggccgcg ggtgagccag gagccccggg tgcaccaggg
     1261 tctcgtggta gatgaactcg tcggcctggc tcgactggat ccgcccgtcc agcacgagac
     1321 acttgccgta cgagtgggtg tccatgatct ccatgaactg gaacttggtc tggcgggtga
     1381 cgatggtccg ggcgatggcg tgcatgtggc cttcgaccgg ggtcgtggtc tcgaagaacc
     1441 acttgtactg gagcgtcgcc atcggccgct cagccgggag tggtgacgac gggcgccccg
     1501 gccggcttgt gcaggagggg ccggccgtgg ttcacgaaga tgccgcgctg gagctcgacc
     1561 accgaggccc gggcggcccc gagctgctcg acgaggtagt cggccgccac ctggggctgg
     1621 agcacgtcgc cgcaggagaa cacgtccacc gcggcgtagc gatactcggg ccaagtgtgg
     1681 atcgccaggt gcgactcggc gatgacgacg actccgctga tgccaaacgg gttgaactcg
     1741 tgaaagacga cgtcgacgat ggtggcttga gcccgcttgg ccgcctccac catggacgcc
     1801 ttcacggtgt ccagactgtt aagggcttcc gcgtcgcagt cgaacaactc gagcagcagg
     1861 tgacgcccca gtgcttgcac tctttccctc ctcccaggtt ctgcccggct ttttttctac
     1921 ttgccggatt tttctgcact gtggggctat ttttttttca ttgcaagaaa aaaatggggt
     1981 tttgggcgcc tccggaggcg ccgcgagcgc tcgccgacgg ctccgcgcga gcgctcgcgc
     2041 gacgcccgcg agcccgccga gcggctccgc gggcaacttt tcgcttgcgt tcgtgggccg
     2101 agccgctacg atgagccaga aattcgcgtc gagccgcgat ggggcgccgc tcgagtcgcg
     2161 tctgctcacg aggccgctca ggaacccgct tacgaggagg agcgaatgca cttcggacca
     2221 gtgaccaagg cggcaggcac cgccggcaac gccgaaggat cgaccccgct caacgccttc
     2281 gacaacgccc tgctggcggc cggcatcggt aacatcaacc tgatcaaggt ctcgagcatt
     2341 ctgcccccgg gcgtcgagct ggtcccgctt ccccgcatca agccgggcgc gctcgtgccc
     2401 accgcctacg cggccatgac gagcgaggtc ccgggcgagg tggtggccgc cgcggtcggc
     2461 tgggcccggc cgaacgatcc cgccaagaac ggggtcatca tggagttcca cgacaaggcc
     2521 acccgcgagg aagccgagcg gatgatcgtg cagatgctcg aggaggcctt ccgggtccgg
     2581 ggcgagacga tccgcgacat gcaggtcttc gcggtcgagc accgggtcga gcggaccggc
     2641 tgcgccctgg ccgcggtcac cctgctctcc gacgacgatc tcgtctagcg ccggctgttg
     2701 atcccggctg agccccgctt catcgcctgc cgggcctccc tcgcggacgc cgggatcgtc
     2761 ctcttcggca tccccttcga aggccgcgtc aacctccgcc gcggcgccga ccacggcccc
     2821 cgcgagctcc ggctggcctc cgactccatc gagacctact ctccgttcct cgggcgggac
     2881 ctcgaagacc tggccatcgc cgatctgggc gactgcgagc tgggcgacgg cacgccccgc
     2941 gagcagctcg cccgggcccg ggaggagatc cgggcgttct ggcggcccgg cctgcggccg
     3001 gtcatgctgg gcggcgacca caccgccacg ctcgcggtca tcgaggtcct ggcgccggcc
     3061 atcccggacc tgcgcatcct ccagctcgac gcccacccgg acctccggga ggagttcctg
     3121 ggcgagcggt acaactacgc ctcggccatg gcccgggtga tggacgtggt cgcccccgag
     3181 cgcgtcttcc aggtcggcat gcggaccggg gcccgggagg agtaccagcg caaggcgccc
     3241 cacctctttc ccgcccaccg ggtgaagccg ctggaggcgg tccggagcct gctgcccgag
     3301 ctccaggcgc acccgctcta cgtgacgatc gacgtcgacg tgctcgaccc gtcgga
//
LOCUS       DATMCV010000054         3176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_468132, whole genome shotgun sequence.
ACCESSION   DATMCV010000054 DATMCV010000000
VERSION     DATMCV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468132"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VNU03_01400"
     CDS             <1..1392
                     /locus_tag="VNU03_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HWP73130.1"
                     /translation="IGVILIRRLEVRPFSEKQIKLLETFADQAVIAIENVRLFQELQA
                     RTQDLSQSLEETGALSEVIRAVSSSLDLREILDTVARRAVALSDADGCGIFELRPGRQ
                     AFDVVVSQGLSAEFVDAVLHLSVGVDHGTISQATESGEPVQIPDVEDAHGYPFRALFL
                     REGLRSVLTVPMGGGDDVARGIVVVRRSAGAFGERVVSLMKALANQSEVAIANANLFR
                     EIEEKSRLLEVANQHKSEFLANMSHELRTPLNAIIGFSEVLLQRMFGELNPKQDEYLQ
                     DVLSSGRHLLSLINDILDLSKVEAGRMELELARFDLPQAMQDTLVLVRERAARHGIDL
                     QLEVDGGVGEIVADERKIKQVMLNLLSNAVKFTPEGGRVDVRTVPTDGGVEISVADTG
                     IGIAAENQELIFEEFRQVGGDYAHKREGTGLGLTLARRFVELHGGRIWVKSQPGLGST
                     FTFSIPERPWPAS"
     gene            1377..1772
                     /locus_tag="VNU03_01405"
     CDS             1377..1772
                     /locus_tag="VNU03_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73131.1"
                     /translation="MAGELILIVEDNEKNRKLERDILQFHGYRTAEADNAEDGIRLAQ
                     ESLPALILMDIQLPRMNGIEALHVLRGDTRTRAVPVIAVTASAMSQDRQKIMAAGFDG
                     YQAKPIDVTGFVTAVREMIDRPGKGGSAR"
     gene            1769..2872
                     /locus_tag="VNU03_01410"
     CDS             1769..2872
                     /locus_tag="VNU03_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73132.1"
                     /translation="MSEPARILVVDDTPMNVKLLEGVLTGHGFVVITAPSGPAALERV
                     RADRPDLVLLDVVMPEMSGYEVCRRLRADEATRLLPIVMVTALDPGTERVKGIEVGAD
                     DFLTKPINQPELLARVRSLLRIKALHDEVGELNRTLERRVAEQVAQLERLGRLRRFFS
                     PQLADLIVSGDAEDPLRSHRREITVVFLDLRGFTAFAETAEPEEVMGVLREYHAEMGR
                     CILAAEGTLERFTGDGMMIFFNDPVVVPDPGPRAVRMAVAMRERIAGLTESWRKRGFD
                     LDFGIGIAQGYATIGAIGFEGRLDYGAIGTVTNLASRLCGEAKPGQILLSQRVASGVE
                     GIAQVEVVGELALKGFQRPVKAYHVVQVGTPTA"
     gene            2869..>3176
                     /locus_tag="VNU03_01415"
     CDS             2869..>3176
                     /locus_tag="VNU03_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HWP73133.1"
                     /translation="MKVCVVGAGAVGTFLGVHLARSGCEVSAVARGQTAAALRTHGLR
                     LQQDGALLAAPVRAAEEASALGRQDLVVVAVKGPSLPAAAAHVGPLLGPETMVLPAMN
                     G"
BASE COUNT          466 a         1083 c         1163 g          464 t
ORIGIN      
        1 atcggcgtga tcctcatccg ccggctcgag gtgcgcccgt tctccgagaa gcagatcaag
       61 ctgctcgaga ccttcgcgga ccaggcggtg atcgcgatcg agaacgtgcg cctcttccag
      121 gagctgcagg cgcgcaccca ggatctctcc cagtcgctgg aggagaccgg cgcgctcagc
      181 gaggtcatcc gcgcggtgag ctcctcgctc gacctgcgcg agattctcga cacggtggcc
      241 cggcgcgcgg tcgcgctctc cgacgccgac ggctgcggga tcttcgagct gcgccccggc
      301 cggcaggctt tcgacgtggt ggtgagccag gggctttccg ccgagttcgt cgacgcggtg
      361 ctgcacctct cggtcggggt cgaccacggc acgatcagcc aggccaccga gtccggcgag
      421 ccggtgcaga tcccggacgt ggaggacgcc cacggctatc ccttccgggc cctcttcctg
      481 cgggaagggc tccgctcggt gctgaccgtg ccgatgggcg gcggcgacga cgtggcgcgc
      541 ggcatcgtgg tggtgcggcg ctcggccggg gcgttcggcg agcgcgtcgt gagcctcatg
      601 aaggcgctcg ccaaccagtc ggaggtggcc atcgccaacg cgaacctctt ccgcgagatc
      661 gaggagaaga gccggctgct cgaggtggcc aaccagcaca agtcggagtt cctcgccaac
      721 atgtcgcacg agctgcgcac tccgctgaac gccatcatcg gcttctcgga ggtgctgctg
      781 cagcgcatgt tcggcgagct gaaccccaag caggacgagt acctccagga cgtgctctcc
      841 tccggtcgtc acctcctctc tctcatcaac gacatcctcg acctctccaa ggtggaggcg
      901 ggccgcatgg agctcgagct ggcccgcttc gacctgcccc aggcgatgca ggacaccctg
      961 gtgctggtgc gcgagcgggc cgcgcgacac ggcatcgacc tgcagctcga ggtggacggc
     1021 ggggtggggg agatcgtcgc ggacgagcgc aagatcaagc aggtgatgct gaatctgctg
     1081 tccaacgcgg tgaagttcac ccccgagggc ggccgggtgg acgtgcggac ggtgccgacc
     1141 gacgggggcg tcgagatctc ggtggcggac accgggatcg ggatcgcggc cgagaaccag
     1201 gagctgatct tcgaggaatt ccgccaggtg ggcggcgact acgcgcacaa gcgcgagggg
     1261 accgggctgg gtctcaccct cgcccgtcgc ttcgtggagc tgcacggggg ccggatctgg
     1321 gtcaagagcc agccgggcct cggctccacc ttcaccttct ccattccgga gcgaccatgg
     1381 ccggcgagct gatcctgatc gtcgaggaca acgagaagaa ccgcaagctg gagcgggaca
     1441 tcctccagtt ccacggctac cgtaccgcgg aggccgacaa cgcggaggat ggaatccggc
     1501 tcgcccagga gagcctgccc gccctcattc tcatggacat tcaattgccg cggatgaacg
     1561 ggatcgaggc gcttcacgtg ctgcggggtg acacgcgcac ccgcgcggtg ccggtgatcg
     1621 cggtcaccgc ctccgcgatg agccaggacc ggcagaagat catggccgcc gggttcgacg
     1681 gctaccaggc caagccgatc gacgtcaccg gcttcgtgac cgcggtgcgg gagatgatcg
     1741 accggcccgg gaagggtggc agcgcgcggt gagcgagccg gcccgcatcc tggttgtgga
     1801 cgacaccccg atgaacgtca agctgctcga gggggtgctg accggccacg gcttcgtggt
     1861 gatcaccgct ccctccgggc ccgccgcgct ggagcgggtg cgcgcggacc ggccggacct
     1921 ggtgctgctg gacgtggtga tgcccgagat gagcggctac gaggtgtgcc ggcggctgcg
     1981 cgccgacgag gcgacccggc tcctgccgat cgtcatggtg accgcgctcg accccgggac
     2041 cgagcgggtg aagggcatcg aggtgggcgc cgacgacttc ctcaccaagc cgatcaacca
     2101 gccggagctg ctcgcccggg tgcggtcgct cctccggatc aaggcgctcc acgacgaggt
     2161 gggcgagctc aaccggaccc tcgagcggcg ggtcgcggag caggtggccc agctcgagcg
     2221 gctcggccgc ctgcgccgct tcttctcgcc ccagctcgcg gacctgatcg tgagcggcga
     2281 cgcggaggac ccgctgcgca gccaccgtcg cgagatcacg gtggtctttc tcgacctgcg
     2341 cggcttcacc gccttcgcgg agactgccga gccggaagag gtgatgggag tgctgcgcga
     2401 gtaccacgcc gagatgggcc ggtgcatcct ggccgccgag ggcacgctgg agcgcttcac
     2461 cggcgacggc atgatgatct tcttcaacga cccggtggtg gtgcccgacc cggggccgcg
     2521 cgcggtccgc atggcggtcg cgatgcggga gcggatcgcg gggctcaccg agtcctggcg
     2581 caagcgcgga ttcgatctcg acttcggcat cggcatcgcg cagggctacg cgaccatcgg
     2641 ggcgatcggc ttcgaggggc ggctcgacta cggcgcgatc ggcaccgtga ccaacctcgc
     2701 ctcgcgcctg tgcggggagg ccaagcccgg gcagatcctg ctctcccagc gggtcgcgtc
     2761 cggcgtggag ggcatcgccc aggtggaggt ggtgggcgag ctcgcgctca agggcttcca
     2821 gcgcccggtg aaggcctacc acgtggtgca ggtcggcacc ccgaccgcgt gaaggtctgc
     2881 gtcgtcggcg cgggcgccgt cggcacgttc ctcggcgtcc acctcgcgcg gagcggctgc
     2941 gaggtctcgg ccgtcgcgcg cgggcagacc gccgccgcgc tgcggaccca cgggctgcgc
     3001 ctccagcagg acggtgcgct gctcgcggcc ccggtccgcg cggccgagga ggccagcgcg
     3061 ctcggacgcc aggacctggt ggtcgtcgcg gtgaaggggc cgtcgctccc cgccgccgcc
     3121 gcgcatgtcg ggcccctgct gggccccgag acgatggtgc tgcccgcgat gaacgg
//
LOCUS       DATMCV010000055         5669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_202523, whole genome shotgun sequence.
ACCESSION   DATMCV010000055 DATMCV010000000
VERSION     DATMCV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202523"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VNU03_01420"
     CDS             complement(<1..824)
                     /locus_tag="VNU03_01420"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWP73134.1"
                     /translation="MTRRHRWLTVAVVMLAGLVISATALWLALPTLARRVVVWQVEAQ
                     TGRRLTMARFELDLRGGQLHVAGFRLADRIPGPPLLEFDRLDVRFHPMRLLRGHLEIA
                     DVTLAGPRVHIVRIGRGELNISDLLARPRSQSEGSPFSLDHLAVKDGAILFEDRTLTP
                     PRTWRAEALTVEADALSSASPEPRGRVRVTTTVAGAPFSLGATGIALRPLQAKAHVSL
                     RDVDATLANLYLPPDTMVALERAVVGAEVDATLDAQGGVGLDGQARITNLVARRRGV"
     gene            complement(886..1821)
                     /locus_tag="VNU03_01425"
     CDS             complement(886..1821)
                     /locus_tag="VNU03_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP73135.1"
                     /translation="MRFGMFTSMGNQTWPAVLEMWKHAEATKWDVACVTDHFMPNTKE
                     REGAMLEGWSTLTALSTLVPRIQVGTIVLGNTYRHPAVVAKMAAQVDIISGGRLLLGL
                     GAGWQENEHEAYGIPFYTMRERLERLDEACQVMKALWTQDRSTFKGRHYQLSDAPLQP
                     KAVQKPHPELMIGGGGEKVTLLTVARYADHWNVWGGPKVLAHKGRILDEHCKSVGRDP
                     RTIRRSANMALLITDKKAEVDALAETIGTRMGRHAADARDTCLAGTPEQIRATLAELR
                     AAKVETLFIPTLFRPLPELRRDLDRFIAEIAPAFR"
     gene            complement(1959..2786)
                     /locus_tag="VNU03_01430"
     CDS             complement(1959..2786)
                     /locus_tag="VNU03_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP73136.1"
                     /translation="MSRATTRPDDDLGLEGRVAIVTGGGAAGDGIGNGRAACILLARS
                     GTSVLVVDRDEGLAKRTVEMITGEGGKASALSVDVTDEAQCAAMAREAVSRYGRLDFL
                     INNVGIGSKGSVVEEKPDTWRRVMQVNVETMFLASKAAIPEMIRTAKGGAIVNISSIS
                     ALRPRGLTTYSASKGAVIALSKAMAVDHGPDGIRVNCIAPGPVYTPMVYARGMTPAAR
                     DQRRRASVLAREGTGWDIGQAARFLLSGHARYITGQVLVVDGGATLVGPARESQTEH"
     gene            complement(2809..3648)
                     /locus_tag="VNU03_01435"
     CDS             complement(2809..3648)
                     /locus_tag="VNU03_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010839058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWP73137.1"
                     /translation="MLATLLDGLTFTEGPRWHDGRLWFSDFYTQRVLAVTPEGQLETI
                     LEVPQRPSGLGWLPDGTLLVVSMLDRQLLRVEDGKPRRHADLASIATAPCNDMVVDAR
                     GRAYVGNFGFDRHRGEAPRAAAIARVDPDGAVTRAAEDLEFPNGTVITPDGRTLVVAE
                     TMGGRLTAFDVAADGALTKRRVWAKLDGVFPDGICLDAECAIWVSDARGPDLLRVREG
                     GAIERRIPTGPGRHAFACTLGGVDRRTLYVCTCTGSGPAMADKRDGRIETVRVDVPGA
                     GLP"
     gene            complement(3661..4434)
                     /locus_tag="VNU03_01440"
     CDS             complement(3661..4434)
                     /locus_tag="VNU03_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HWP73138.1"
                     /translation="MISVFALCSGYLELDRASMLDDLPAGQPWTVPVMSFLVGHPRGR
                     LLFDTGVHCQTRVDPIGRMGPERAKRLVDKSKAGEDVVPQLGLLGLTPDDVRYVVNSH
                     LHFDHCGGNEFFPRATFLVQKAEMEAARRPGFAPGYNPSPLDFDHPLDYRLVDGEHDV
                     WGDGSVILFPTFGHTPGHQSLRVRAGKGQDLVCAADACYTRENMDRDVLPRILWNGST
                     MRESLATLRRLRDQAGATVFYGHDPEQWRATPHAPAAVV"
     gene            4555..5523
                     /locus_tag="VNU03_01445"
     CDS             4555..5523
                     /locus_tag="VNU03_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HWP73139.1"
                     /translation="MVYRRGSKGPEVTRIQAQLRARGLYLGPADGDFGGGTESAVLAF
                     QRAEGLVPDGLVGPETWSRLFGGGEIPAPAITKEALDYRSLALTGAFETDAAVPECFA
                     GLSGNFDGQGISFGALQWNLGQGSLQPLLRQMDTQHPAIVEEVFGPSTAELRAMLSAP
                     LAEQLTWATSIQDPRRSVVNEPWRGRFKTLGRREEFQLIQTAAAHAMYQEALTWCGAY
                     GVASERAVALLFDIRVQNGSISALTRTQIQQDFTRIPGTADPEAAEVARLRAIANRRA
                     EAASPRWIEDVRARKLTIAEGIGTVHGLHFDLAGQYGIGLRRAAGL"
BASE COUNT          776 a         1994 c         2104 g          795 t
ORIGIN      
        1 actccgcggc ggcgcgccac caggttggtg atgcgcgcct ggccgtcgag tccgacgccg
       61 ccctgcgcgt cgagcgtggc gtcgacctcc gcgcccacca ccgcgcgctc cagcgccacc
      121 atggtgtcgg gcggcaggta caggttggcg agggtggcat cgacgtcgcg caggctgacg
      181 tgggccttcg cctgcagggg gcgcagcgcg atgccggtgg cgcccagcga gaacggggcg
      241 cccgccacgg tggtggtgac ccgcacgcgg ccgcgcggct ccgggctcgc gctggagagc
      301 gcgtcggcct cgacggtgag ggcctcggcg cgccaggtgc gcggcggggt cagggtgcgg
      361 tcctcgaaca ggatcgcgcc atccttcacc gcgaggtggt ccagcgagaa cggcgagccc
      421 tccgactgcg agcgtgggcg cgcgagcagg tccgagatgt tcagctcgcc ccgcccgatg
      481 cgcacgatgt ggacgcgcgg gccggcgagc gtcacgtccg cgatctccag atggccccgc
      541 agcaggcgca tcggatggaa gcgcacgtcg aggcgatcga actccagcag gggcggcccg
      601 gggatccgat ccgcgaggcg gaatcccgcc acgtgcagct gcccgccgcg gaggtcgagc
      661 tcgaagcggg ccatggtgag ccggcggccg gtctgggcct ccacctgcca caccaccacc
      721 cggcgggcca gggtcggcag cgcgagccag agcgcggtgg cggagatcac caggcccgcg
      781 agcatgacga ccgcgacggt gagccagcgg tggcggcgag tcacgtcccc agtatgcctc
      841 gccggccgtc ccgggaccta ctgaccggaa ccggtggacc cgggctcagc ggaaggcggg
      901 ggcgatctcc gcgatgaagc ggtcgaggtc ccggcgcagc tccggcaggg gccggaagag
      961 ggtcgggatg aacagggtct cgaccttcgc cgcccgcagc tcggccagcg tggcccggat
     1021 ctgctccggg gtgccggcca ggcaggtgtc gcgcgcgtcc gcggcgtggc gccccatgcg
     1081 ggtgccgatg gtctcggcga gcgcgtcgac ctccgccttc ttgtcggtga tcagcagggc
     1141 catgttggcc gagcggcgga tggtcctggg atcgcggcct acgctcttgc agtgctcgtc
     1201 gaggatgcgg cccttgtggg cgaggacctt ggggccgccc cacacgttcc agtgatccgc
     1261 gtagcgggcc accgtgagga gcgtcacctt ctcgccgccg ccgccgatca tcagctcggg
     1321 gtggggcttc tgcaccgcct tgggctgcag cggggcgtcc gagagctggt agtggcggcc
     1381 cttgaaggtg gagcggtcct gcgtccacag cgccttcatc acctggcagg cctcgtcgag
     1441 ccgctcgagg cgctcgcgca tcgtgtagaa ggggatgccg taggcctcgt gctcgttctc
     1501 ctgccagccc gcgccgaggc cgagcagcag ccggccgccc gagatgatgt ccacctgcgc
     1561 ggccatcttg gccaccaccg cggggtggcg ataggtgttg ccgagcacga tggtgccgac
     1621 ctggatgcgc ggcaccaggg tggagagcgc ggtgagcgtg ctccagccct cgagcatcgc
     1681 gccctcgcgc tccttcgtgt tgggcatgaa gtggtcggtc acgcacgcca cgtcccactt
     1741 cgtggcctcc gcgtgcttcc acatctcgag gaccgccggc cacgtctggt tgcccatgct
     1801 cgtgaacatg ccgaagcgca tcgcagatct ccttgagagt tgggtcctag cgcgacctat
     1861 tcatgaacgg gcaaccgggg ggcccactac gctcgtctgc gcgggaccca cccccggcgc
     1921 cacccggccg atctccctcg aggccgttca tgaatgctct agtgctcggt ttgagattct
     1981 ctggcggggc cgaccagggt ggcgccgccg tccaccacaa gcacctggcc ggtgatgtaa
     2041 cgggcgtggc ccgacagcag gaagcgggcg gcctggccga tgtcccagcc ggtgccctcg
     2101 cgggcgagga cggaggcgcg ccgccgctgg tcgcgcgccg cgggggtcat gccgcgggcg
     2161 tagaccatcg gggtgtagac cgggccgggg gcgatgcagt tgacccggat gccgtcgggg
     2221 ccgtggtcca ccgccatcgc tttgctcagc gcgatcaccg cgcccttgga ggccgagtag
     2281 gtggtgaggc cgcgcgggcg cagggccgag atcgaggaga tgttcacgat ggcgccgccc
     2341 ttggcggtgc ggatcatctc cgggatcgcg gccttggagg cgaggaacat ggtctcgacg
     2401 ttgacctgca tgacccgccg ccaggtgtcg ggcttctcct ccaccacgct gcccttgctg
     2461 ccgatcccca cgttgttgat caggaagtcg aggcggccgt agcggctcac cgcctcgcgg
     2521 gccatcgccg cgcactgcgc ctcgtcggtg acgtccaccg agagggccga cgccttgccg
     2581 ccctctccgg tgatcatctc gacggtgcgc ttggcgaggc cctcgtcgcg atcgaccacc
     2641 agcacgctgg tgccgctgcg cgcgagcagg atgcaggccg cgcgcccgtt gccgatgccg
     2701 tcgccggcgg cgccgccgcc ggtgacgatg gccacccgtc cctccagtcc caggtcgtcg
     2761 tcgggccgcg tcgtcgctct actcatgggg atgcctttcg tgggtgcgtc aggggaggcc
     2821 cgcgccgggc acgtccactc ggacggtctc gatgcggccg tcgcgcttgt cggccatggc
     2881 gggcccgctg ccggtgcagg tgcacacgta gagggtgcgg cgatccacgc cgcccagcgt
     2941 gcacgcgaag gcgtggcgtc cgggcccggt ggggatgcgc cgctcgatcg cgccgccctc
     3001 acggacccgc aggagatcgg gcccgcgcgc gtcgctgacc cagatcgcgc actcggcgtc
     3061 caggcagatc ccgtccggga agacgccgtc gagcttcgcc cagacgcgcc gcttcgtcag
     3121 cgccccgtcg gccgccacgt cgaaggcggt gagcctgccg cccatggtct cggccaccac
     3181 gagcgtccgg ccgtcggggg tgatcacggt gccgttcggg aactcgagat cctccgccgc
     3241 gcgggtgacc gcgccgtcgg gatcgacgcg cgcgatggcc gcggcgcggg gcgcctcgcc
     3301 gcggtggcgg tcgaagccga agttgccgac gtaggcgcgg ccgcgcgcgt ccaccaccat
     3361 gtcgttgcag ggggcggtgg cgatcgacgc gagatcggcg tgccggcgcg gcttgccgtc
     3421 ctccacgcgg aggagctggc gatcgagcat cgagaccacg agcagggtgc cgtccggcag
     3481 ccagccgagg cccgacggcc gctgcggcac ctcgaggatg gtctcgagct ggccttccgg
     3541 ggtcaccgcg aggacgcgct gggtgtagaa gtccgagaac cagagccgcc cgtcgtgcca
     3601 gcgcggccct tcggtgaagg tcagcccgtc gagcagcgtc gcgagcacgg cggtcgcggc
     3661 ctagacgacg gcggcgggcg cgtgcggcgt ggcgcgccac tgctcggggt cgtggccgta
     3721 gaacacggtg gccccggcct gatcacgcag gcggcgcagc gtggcgagcg actcgcgcat
     3781 ggtcgagccg ttccacagga tgcgcgggag cacgtcgcga tccatgttct cgcgcgtgta
     3841 gcaggcgtcg gccgcgcaga ccaggtcctg gcccttcccg gcccgcaccc ggagggactg
     3901 gtggccgggg gtgtggccga aggtggggaa caggatcacg ctgccgtcgc cccacacgtc
     3961 gtgctcgccg tcgaccaggc ggtagtcgag gggatggtcg aagtcgagcg ggctcggatt
     4021 gtagccgggg gcgaaccccg gccggcgcgc cgcctccatc tccgccttct gcacgagaaa
     4081 ggtcgcgcgc ggaaagaact cgttgccgcc gcagtggtcg aagtggaggt gcgagttcac
     4141 cacgtagcgc acgtcgtccg gggtgaggcc gaggaggccg agctggggga ccacgtcctc
     4201 gccggccttc gacttgtcga cgagccgctt ggcccgctcg gggcccatgc gtccgatcgg
     4261 atcgacgcgc gtctggcagt ggacgccggt gtcgaagagg agccgcccgc gcgggtgccc
     4321 gaccaggaag ctcatcaccg ggaccgtcca cggctggccg gccgggagat cgtccagcat
     4381 gctggcgcgg tccagctcga gatagccgct gcagagggcg aagaccgaga tcacggcggc
     4441 tacgataaac cctcgcgccg ccgttgtcag gtggaaatgc ggcggccgcg cgcgcgcgca
     4501 gtaagggcag atttcccttg cgccgcgggt cacccggcgc atcatcggcg catcatggtg
     4561 taccgccgcg gatcgaaggg ccccgaggtt acccggatcc aggcgcagct gcgagcgcgg
     4621 gggctctacc tcgggccagc ggacggcgac ttcgggggcg gcaccgagag cgcggtgctc
     4681 gccttccagc gcgccgaggg actcgtcccc gacggcctcg tgggacccga gacgtggagc
     4741 cgactcttcg gcggcgggga gatcccggcc ccggcgatca ccaaggaggc cctggactac
     4801 cgcagcctcg cgctcaccgg ggccttcgag accgacgcgg cggtcccgga gtgcttcgcc
     4861 ggcctctccg gcaacttcga cggccagggc atcagcttcg gcgcgctgca gtggaacctg
     4921 ggccagggca gcctgcagcc gttgctgcgc cagatggaca cccagcatcc cgccatcgtg
     4981 gaggaggtct tcggcccgag cacggcggag cttcgcgcga tgctgagcgc gcccctcgcc
     5041 gagcagctca cgtgggccac gtccatccag gatccgcgtc gttcggtggt gaacgagccg
     5101 tggcgcggcc ggttcaagac cctcggccgc cgcgaggagt tccagctgat ccagaccgcg
     5161 gccgcccacg ccatgtacca ggaggccctt acctggtgcg gcgcctacgg cgtggccagc
     5221 gagcgtgcgg tggccctgct gttcgacatc cgggtgcaga acggtagcat ctccgccctg
     5281 acccgcacgc agatccagca ggacttcacc cggatccccg ggaccgccga tcccgaggcc
     5341 gccgaggtcg cccgcctgcg cgcgatcgcc aaccggcggg ccgaggcggc cagcccgcga
     5401 tggatcgagg acgtgcgcgc acgcaaactc accatcgccg aggggatcgg caccgttcac
     5461 ggcttgcact tcgatctcgc gggccaatac gggatcgggc tgcgccgggc ggccggactc
     5521 taggctccgg aggatccccc atggcgtggc cgttcgagcc cacgaatcga ggcttctcgc
     5581 tcacgaacgc gaagggaagc gcgctggccg cccgcctcgc ctaccaggac cctgcgaccg
     5641 tcgaccgcac cacgagcgcc gactgggga
//
LOCUS       DATMCV010000056         3394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_934541, whole genome shotgun sequence.
ACCESSION   DATMCV010000056 DATMCV010000000
VERSION     DATMCV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934541"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VNU03_01450"
     CDS             complement(<1..580)
                     /locus_tag="VNU03_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73140.1"
                     /translation="MPLSMFTDEHEMLRRTVRAFVEKEVAPHVDAWEEAGRIPREFWR
                     RLGELGLLGLEFPVEYGGGGGDFLSSVVLGEEMARCRSGGVAFSVLVHTDMSSPWLTR
                     YGTEAQKRAYLPGIVRGETVCALGITEPGTGSDMAGIATRAVRDGDHYLLTGSKIFIT
                     NGVYGDLYFVAARTGAGTADRRHAGISMLLVEG"
     gene            complement(591..1688)
                     /locus_tag="VNU03_01455"
     CDS             complement(591..1688)
                     /locus_tag="VNU03_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HWP73141.1"
                     /translation="MAQSRVRAVYMRGGTSRCLAFHDRDLPPAGPARDRILLAALGSP
                     DPSGRQLDGLGGGISSLSKAFIIGAPTHPDAQVDYTFAQVEIGRPVVDYTGNCGNCSS
                     AVGPFAIDERIVPYANGETAVRIHNTNTKKIIVARVPVVDGEAAVLGDFELPGVAGHG
                     ARISLDFLEPGGSRTGRLLPSGRTREMIAGVEASMVDATNPMVFVRAKDLGLTGTEPP
                     AAMDGDKALVARLEAIRVAAAEAMGIPGSAATPKLAVVAPPLRFTALDGVAYDAEQTD
                     VVARCISMGNAHRAFALTAAMCLAVAAQIDGTVVSECAGGRVGDLRLGHPSGVLPIAA
                     TVGQRDGAAWAERVTVYRTARRLMEGYVRVP"
     gene            complement(1688..>3394)
                     /locus_tag="VNU03_01460"
     CDS             complement(1688..>3394)
                     /locus_tag="VNU03_01460"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HWP73142.1"
                     /translation="WFSKPGNGICHQVHFESFSVPGEMLLGTDSHSPLCGSTGMLAIG
                     AGGLDVAVAMGGGPYFLAMPRVTSVWLSGRLRPWVSAKDVIMELLRRYTVRGGSGRIF
                     EYGGPGAATLSLPQRATICNMGAELTLTTSVFPSDEATREYFRLLGREAEWRPLGPDP
                     DAEYDDRIELDLDAIEPLVALPGSPDRVVPVSEVEGEPIEQVMVGSCTNSSWEDMQAV
                     TRVLEGRRVAPSCTFVVFPGSHRILEVMAREGLLTTLLAAGASISEPTCGACAGIGHV
                     PASGGRSLRAFNRNFPGRSGTKDDAVYLCSPLTAAASALTGRITDPRKAGVAPERRWP
                     ASLLASVAGLIPPTPEAEAATVEVLKGPNIKPVPRGTPVEERLLAPVLIKLGDKVSTD
                     DISPSGTAVLMFRSNVPAIAEFCFRNVDAEFVARARAAGSGVIVGGEIYGQGSSREAA
                     VLSPLHLGVRAVLAKSFARIHRANLINWGIVPLEFESPADYDGIERDDVLELPDLRES
                     LAAGGLVAVVNHRTGARFRMRSILSPRERDMLLAGGLLTQTASISSSPPSGGEGRVRG
                     RI"
BASE COUNT          474 a         1203 c         1279 g          438 t
ORIGIN      
        1 cgccctcgac gaggagcatc gagatgccgg cgtggcggcg gtccgcggtg ccggcgccgg
       61 tgcgcgcggc cacgaagtag aggtcgccgt acacgccgtt ggtgatgaag atcttgctcc
      121 cggtgagcag gtagtggtcg ccgtcgcgca ccgcgcgggt cgcgatgccc gccatgtcgg
      181 agccggtgcc gggctcggtg atgccgagcg cgcacaccgt ctcgccgcgg acgatgccgg
      241 gcaggtaggc gcgcttctgc gcctcggtgc cgtagcgggt gagccagggc gaggacatgt
      301 cggtgtgcac cagcacgctg aaggccaccc cgccggagcg gcagcgcgcc atctcttccc
      361 ccagcaccac gctcgagagg aagtcccccc cgccgcctcc gtactcgacc gggaactcga
      421 ggccgagaag ccccagctcg cccagccgac gccagaactc gcgggggatg cgtccggcct
      481 cctcccacgc gtccacgtgg ggcgcgacct ccttctccac gaacgcgcgg acggtccgcc
      541 gcagcatctc gtgctcgtcg gtgaacatcg agagcggcat cgtcggcggg tcagggcacc
      601 cgcacgtagc cttccatcag gcggcgcgcg gtccggtaca ccgtcacgcg ctcggcccac
      661 gcggcgccgt cccgctgtcc caccgtcgcc gcgatcggca gcaccccgga gggatggccg
      721 agccgcaggt cgccgacccg gccgcccgcg cactcgctca ccacggtgcc gtcgatctgc
      781 gccgcgaccg ccaggcacat ggcggcggtc agcgcgaagg cgcggtgggc gttgcccatc
      841 gagatgcagc gcgcgaccac gtcggtctgc tccgcgtcgt aggcgacccc gtcgagcgcg
      901 gtgaagcgga gcgggggcgc caccaccgcg agcttcgggg tggccgcgct gcccgggatg
      961 cccatggcct cggccgcggc cacccggatc gcctcgagcc gcgcgacgag ggccttgtcg
     1021 ccgtccatcg cggcgggcgg ctcggtgccg gtcagcccga gatccttggc ccgcacgaag
     1081 accatgggat tggtggcgtc gaccatcgag gcctcgacgc ccgcgatcat ctcgcgggtc
     1141 cgtccgctcg gcagcagccg gccggtgcgg gagcccccgg gctcgaggaa gtcgagcgag
     1201 atgcgggctc cgtgcccggc caccccgggc agctcgaagt cgccgagcac cgcggcctcg
     1261 ccgtccacca ccggcacgcg ggccacgatg atcttcttgg tgttggtgtt gtggatgcgg
     1321 accgcggtct cgccgttggc gtacggcacg atccgctcgt cgatggcgaa ggggcccacc
     1381 gccgaggagc agttcccgca gttgccggtg tagtcgacga ccggtcggcc gatctcgacc
     1441 tgcgcgaacg tgtagtcgac ctgggcgtcg ggatgggtgg gcgcgccgat gatgaaggcc
     1501 ttggagagcg aggagatccc gccgcccagc ccatcgagct ggcggccgga cggatcgggg
     1561 ctgccgagcg cggcgaggag gatgcggtcg cgcgcggggc cggcgggcgg caggtcgcga
     1621 tcgtggaagg ccaggcagcg gctggtgcca ccgcgcatgt agacggctcg gactcgagac
     1681 tgggccatta gattctcccc ctcaccctgc cctctccccc cgatggggga gaggaggaaa
     1741 tggaggcggt ctgggtgagc aggccgccgg ccaggagcat gtcgcgctcg cgggggctca
     1801 ggatcgagcg catgcggaag cgcgcgccgg tgcggtggtt caccaccgcg accaggccgc
     1861 ccgcggcgag gctctcgcgc agatcgggga gctcgagcac gtcgtcgcgc tcgatgccgt
     1921 cgtagtcggc cgggctctcg aactcgagcg gcacgatgcc ccagttgatc aggttcgcgc
     1981 ggtggatgcg ggcgaagctc ttggcgagca ccgcacggac ccccaggtgg aggggggaga
     2041 gcaccgccgc ctcgcgcgag gagccctggc cgtagatctc gccccccacg atgaccccgg
     2101 agccggccgc gcgggcacgg gcgacgaact cggcgtccac gttgcggaag cagaactcgg
     2161 cgatggcggg cacgttggag cggaacatca gcaccgcggt gccggagggc gagatgtcgt
     2221 cggtcgagac cttgtcgccg agcttgatca gcaccggcgc gaggaggcgc tcctcgaccg
     2281 gcgtcccgcg gggcaccggc ttgatgttcg ggcccttcag gacctcgacc gtcgcggctt
     2341 cggcctcggg ggtgggcggg atcagccccg ccaccgacgc gagcagcgac gcgggccacc
     2401 gccgctcggg ggcgacgccc gccttccgcg ggtcggtgat ccggccggtg agcgcggagg
     2461 cggccgcggt cagcggcgag cagaggtaga ccgcgtcgtc cttggtgccg ctccgccccg
     2521 ggaagttgcg gttgaaggcc cgcaggctgc ggccgcccga ggcgggtacg tggccgatgc
     2581 ccgcgcacgc cccgcaggtg ggctccgaga tgctggcccc ggccgcgagc agggtggtga
     2641 gcaggccctc gcgcgccatc acctcgagga tgcggtggct gcccgggaag accacgaagg
     2701 tgcacgaggg cgccacgcgc cggccctcga gcacgcgcgt caccgcctgc atgtcctccc
     2761 acgaggagtt ggtgcaggag ccgaccatca cctgctcgat cggctcgccc tcgacctcgg
     2821 agaccgggac cacccggtcc ggcgagccgg gcagggcgac cagcggctcg atcgcgtcga
     2881 ggtcgagctc gatgcggtca tcgtactcgg cgtccggatc cggcccgagc ggccgccact
     2941 ccgcctcgcg cccgaggagg cggaagtact cgcgggtcgc ctcgtcggag gggaacacgc
     3001 tggtggtgag ggtgagctcg gcgcccatgt tgcagatggt ggcccgctgc ggcagcgaga
     3061 gagtcgccgc cccgggtccg ccgtactcga agatgcgccc gctgccgccg cgcaccgtgt
     3121 aacggcgcag cagctccatg atcacgtcct tggccgacac ccacggacgc aggcggccgc
     3181 tgagccacac cgaggtgacg cgcggcatgg ccaggaagta cggcccgccg cccatggcca
     3241 ccgccacgtc gaggccgccc gctccgatgg cgagcatgcc ggtggagccg cagagcgggc
     3301 tgtggctgtc ggtgccgagg agcatctcgc cggggacgct gaacgactcg aagtgcacct
     3361 ggtggcagat gccgttgccg ggcttggaga acca
//
LOCUS       DATMCV010000057         2017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_403389, whole genome shotgun sequence.
ACCESSION   DATMCV010000057 DATMCV010000000
VERSION     DATMCV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403389"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2017)
                     /locus_tag="VNU03_01465"
     CDS             complement(<1..>2017)
                     /locus_tag="VNU03_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP73143.1"
                     /translation="VLERMMEAVHRYEGTVNQILGDGIMAIFGAPLAHEDHAVRACHA
                     ALRMQQTVGWYAEELRRGRGVDVQIRVGLNSGEVVVRAIDSDLHMDYSAIGQTTHLAA
                     RMEQLARPGSTLVSRETLRLAEGYIEVKPLGPVPVKGMPEPVEIFELVRTEQVHSRLE
                     ASAARGLTPFVGREAEVDSLHQAMARASAGHGQIVAVSGEPGVGKSRLFWEFTHSHRA
                     HGWLILEAQATSYGKNTSYLTVKELLQSYFQLEERDDPRRVREKVTGKLLTLDESLRP
                     TLPAFLQLLDVPVDDAEWQTPDLPQRRQRMLDAVKRLILREGQVQPVIAVFENLHWLD
                     SESQAFLDSLVDSLPLARALILVSYRPEYQHGWARKSYYTQFRIDPLPPQSAEELLRA
                     LVGDDPGLGPLKQLLIERTEGNPFFLEESVHTLAETKVLAGNRGHYRLTREVQKVQVP
                     ATVQAVLAARIDRLPIEAKRLLQAGAVIGKDVSLALLQEIADLSEEATRRNLALLQSG
                     EFLYEISLFPSVEYTFRHALTHEVAYSSLLQDRRRALHGRVVTAIEQLYADRLPEHFE
                     RLAHHAVRGEVWAKAVAYLRQAGIKAAGRSSNREAVIFLEQAMAALTRLPEGRAATEQ
                     AIDLRLDLRSPLLQSGQLERVLTLSQEAEAMAEKLGDEQRLARVYTYL"
BASE COUNT          279 a          719 c          704 g          315 t
ORIGIN      
        1 tcaggtaggt atagacgcgc gccagccgct gctcgtcgcc gagcttctcg gccatcgcct
       61 ccgcttcctg cgagagcgtc agcacccgct cgagctgccc cgactggagc agcggcgagc
      121 gcaggtccag gcgcaggtcg atggcctgct cggtcgccgc gcggccctcg ggcagccggg
      181 tgagcgcggc catggcctgc tcgaggaaga tgaccgcctc ccggttggag gagcgtcccg
      241 ccgccttgat gccggcctga cgcaggtacg ccaccgcttt cgcccacacc tcgccgcgca
      301 ccgcgtggtg ggcgagccgc tcgaagtgct cgggcagccg gtcggcgtag agctgctcaa
      361 tggcggtcac cacgcgcccg tggagcgcgc ggcgccgatc ctggagaagg ctcgagtagg
      421 ccacctcgtg ggtcagcgcg tggcggaacg tgtactccac gctcgggaag aggctgatct
      481 cgtacaggaa ctcgccggac tggagcaggg ccaggttgcg gcgcgtcgcc tcctccgaca
      541 ggtcggcgat ctcctgcagc agcgccagcg acacgtcctt gccgatgacc gcgcccgcct
      601 gcagcagccg cttggcctcg atgggcaggc gatcgatgcg cgcggccagc accgcctgca
      661 cggtggcggg cacctggacc ttctggactt ctcgcgtgag gcgatagtgg ccgcgattgc
      721 ccgcgagcac cttggtctcg gcgagggtgt gcacgctctc ttccaggaag aacgggttgc
      781 cctcggtgcg ctcgatcagg agctgcttga gcggccccag gcccgggtcg tcgcccacca
      841 gcgcccgcag cagctcctcc gcgctctggg gcggcagggg atcgatgcgg aactgggtgt
      901 agtagctctt gcgggcccac ccgtgctggt attcgggccg gtagctcacc aggatgagcg
      961 cgcgggcgag cgggaggctg tccaccaggc tgtcgaggaa cgcctggctc tccgaatcaa
     1021 gccagtgcag gttctcgaag accgcgatca ccggctgcac ctggccttcg cgcaggatca
     1081 ggcgcttgac cgcgtcgagc atccgctgcc ggcgctgcgg caggtcggga gtctgccatt
     1141 ccgcgtcgtc caccggcacg tccaggagct gcaggaacgc gggcagggtc ggccgcaggc
     1201 tctcgtcgag ggtcaggagc ttgccggtga ctttctcgcg aacccgcctc gggtcgtccc
     1261 gttcctcgag ctggaagtag ctctgcaaga gctccttcac cgtgaggtag gacgtgttct
     1321 tgccgtagga cgtggcctgg gcttccagga tgagccagcc gtgggcccgg tgagagtggg
     1381 tgaactccca gaagagccgc gacttgccga ccccgggctc gcccgagacc gcgacgatct
     1441 gcccgtgccc ggccgaggcc cgcgccatcg cctggtggag gctgtccacc tcggcctcgc
     1501 ggcccacgaa cggggtgagc ccgcgcgccg cgctggcctc gaggcgcgag tgcacctgct
     1561 cggtgcgcac cagctcgaag atctccaccg gctccggcat ccccttcacc ggtaccggtc
     1621 cgagcggctt gacctcgatg tagccctcgg ccaggcgcag cgtctcacgc gacaccagcg
     1681 tcgatccggg ccgcgcgagc tgctccatgc gcgcggccag gtgggtcgtc tggccgatgg
     1741 ccgagtagtc catgtgcagg tcgctgtcga tggcccgcac caccacctcg ccggagttga
     1801 gcccaacgcg gatctgcacg tccaccccgc gcccgcgccg cagctcctcc gcgtaccagc
     1861 ccaccgtctg ctgcatgcgc agcgcggcgt ggcaggcgcg caccgcgtgg tcctcgtggg
     1921 cgagcggcgc gccgaagatc gccatgatcc cgtcgccgag gatctggttg acggtgccct
     1981 cgtaccggtg gaccgcctcc atcatccgct cgaggac
//
LOCUS       DATMCV010000058         4403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_468573, whole genome shotgun sequence.
ACCESSION   DATMCV010000058 DATMCV010000000
VERSION     DATMCV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468573"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VNU03_01470"
     CDS             <1..567
                     /locus_tag="VNU03_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP73144.1"
                     /translation="TGLRGKTLGILGYGKIGAVVAGYGRAFGMNVIAWGRESTLEKAK
                     TDGYTPAASREAFFADSDVVSIHVRLIDATRGMVTPEDLARMKPSAVFVNTSRAGLVA
                     PGALEAALAKGRPGMAAVDVYEDEPVLGAKHPLLAMDNAICVPHLGYVERDGLEHMFD
                     TIFDQVLAYGAGKPINVLNPEALAAARS"
     gene            564..1733
                     /locus_tag="VNU03_01475"
     CDS             564..1733
                     /locus_tag="VNU03_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWP73145.1"
                     /translation="MSIRDRAYVVGAFEHPTRKADGISLAQLHAEVARGALADAGLTK
                     DDVDGYFCAGDAPGLGGLSMADYMGLRLRHIDTTETGGSSYVVHVAHAAEAIAAGRCD
                     VALITLAGRPRAEGMATGTAPRNYGAAAPDVAFEAPFAPTVVNQYAMAAMRHMHEFGT
                     TSEQLAWIKVAASHHAQHNPHAMLREVVTVADVVGSPMISDPLHRLDCCVISDGGGAV
                     VVARSEVARGLKRPLVTVRGAGEAVKHQMGGQVDLTYTGARWSGPAAFEMAGVAPTDI
                     KYASIYDSFTITVLITLEDLGFCPKGKGGRFVSDGNLIAGTGRLPFNTDGGGLCNNHP
                     ANRGGMTKVIEAVRQLRGEAHPAVQVKGCDLALAHGTGGSLGTRHASATLIMERV"
     gene            1737..2129
                     /locus_tag="VNU03_01480"
     CDS             1737..2129
                     /locus_tag="VNU03_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HWP73146.1"
                     /translation="MADAKRTILAPTPSPETAPFWDAATKGRLLLKRCTACGRAHHYP
                     RPICPFCGSDRTEWTESPGRGVIYSFSVMRRAAPPYVIAYVTLDEGVTMMTGLVECDF
                     DALRIGQKVRVVFVPSDGGPPVPMFRPA"
     gene            complement(2152..4131)
                     /locus_tag="VNU03_01485"
     CDS             complement(2152..4131)
                     /locus_tag="VNU03_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWP73147.1"
                     /translation="MAKLTLAQACCNNEVAYLAWQTDGKIAGCLGFMITRIHLDATGH
                     EVERRILPAWVAFETQSNPDWEPQDTSVWPIQKFSWRDLTLRRSRNTATTRPPDFKAK
                     YEIVPVGLPGPGRAPVSLSPTAQPGKYKGTPILLFVCGAAIQTNEFLVTGDFGDISVA
                     FNNGILSTQNLRKQLKTPKGVAPTKKDIEGHIAKEGDPIREFLAGDVLPALRSLFERA
                     DREDATVRLALYELEDKELLGLLVKHQQRLQIILSTAGSTPPKKGSGDPVKWDTTNTG
                     ARKKLHALMGDRLQNRMFNNSAHIGHNKFAVLIAKNGTPTAVWTGSTNWTKTGLCAQT
                     NNTIMLESKTVAQAYLDYWNRLHADKLPTPKPLSAPLASDQGTPLRTADAKSTPVPLD
                     GNATHVDLWYSPNTPKTGTSQNRTVPPDLAMVFDLMKKAKEAIFFLVFNPGRSDPAGE
                     DVNTVVSAGIDFGRLDPNLLVTGAISDPTAVPGYVTPPKGQKKDPKKPKIPQIAIFSP
                     NGAPNVLMIRAAAINDLIGDFQKELLSAGHAIIHDKIVVIDPLSKTDCTVITGSHNLG
                     FKASYANDENMVIVRGNRPLALAYAVHVLDVYDHFKFRAALEQQLRDALLAGRTPPKK
                     PTGKGFLDTDDKWQDPYISGQKGKEPRYFLSVPLP"
BASE COUNT          633 a         1449 c         1586 g          735 t
ORIGIN      
        1 accgggctgc gaggcaagac cctgggcatc ctgggctacg gcaagatcgg cgcggtggtg
       61 gcgggctacg ggcgcgcgtt cggcatgaac gtgatcgcgt ggggtcgcga gagcacgctc
      121 gagaaagcca agaccgacgg ctacaccccc gccgcgagcc gcgaggcctt cttcgccgac
      181 tccgacgtgg tctcgatcca cgtgcggctg atcgacgcga cccgcgggat ggtgaccccg
      241 gaggacctcg cgcggatgaa gccctccgcc gtcttcgtca acacgagccg cgccgggctg
      301 gtggcgccgg gcgcgctcga ggccgcgctg gccaagggcc ggcccggcat ggccgcggtg
      361 gacgtctacg aggacgagcc ggtgctgggg gccaagcacc cgctgctcgc gatggacaac
      421 gcgatctgcg tgccgcatct gggctacgtg gagcgcgacg gcctcgagca catgttcgac
      481 accatcttcg accaggtcct ggcctacggg gcgggcaaac cgatcaacgt gctgaacccg
      541 gaggctctcg cggcggcccg gtcatgagca ttcgcgaccg cgcgtacgtc gtcggcgcct
      601 tcgagcatcc gacccgcaag gcggacggca tctcgctggc ccagctccac gcggaggtgg
      661 cgcggggcgc gctcgccgac gccgggctca ccaaggacga cgtggacggc tacttctgcg
      721 cgggcgacgc ccccggcctg ggcgggctct ccatggccga ctacatgggc ctgcgcctgc
      781 ggcacatcga caccaccgag accgggggct cgtcgtacgt ggtgcacgtg gcccacgccg
      841 ccgaggccat cgcggccggc cgctgcgacg tggccctgat cacgctggcc ggccgcccgc
      901 gcgcggaggg catggccacc ggcaccgcgc cccgcaacta cggcgcggcc gcgcccgacg
      961 tggcgttcga ggccccgttc gcccccaccg tggtgaacca gtacgcgatg gccgcgatgc
     1021 gccacatgca cgagttcggg accaccagcg agcagctcgc gtggatcaag gtcgcggcct
     1081 cgcatcacgc gcagcacaat ccgcacgcga tgctgcgcga ggtcgtcacc gtcgcggatg
     1141 tggtgggctc gccgatgatc tcggacccgc tgcaccgtct ggactgctgc gtgatcagcg
     1201 acggaggcgg tgcggtggtg gtggcacgct cggaggtggc gcggggcctc aagcgcccgc
     1261 tcgtcacggt ccgcggggcg ggcgaggcgg tgaagcacca gatgggcggc caggtcgacc
     1321 tcacctatac cggcgcgcgc tggtcgggcc cggccgcctt cgagatggcg ggcgtggctc
     1381 ccaccgacat caagtacgcc tcgatctacg atagcttcac gatcacggtg ctgatcaccc
     1441 tggaggatct gggcttctgc cccaagggga agggcggccg gttcgtgtcg gacggcaacc
     1501 tgatcgcggg gacgggccgg ctgcccttca acaccgacgg cggcggcctc tgcaacaacc
     1561 acccggccaa ccggggcggc atgaccaagg tgatcgaggc ggtgcgccag ctgcggggcg
     1621 aggcgcatcc ggcggtacag gtgaagggct gcgacctggc gctcgcccac ggcaccggcg
     1681 gctcgctcgg cacacgccac gcgagcgcga ccttgatcat ggagagggtc tgagccatgg
     1741 cggacgcgaa gcggacgatc ctggcgccca ccccgagccc ggagaccgcg cccttctggg
     1801 acgcggcgac caagggccgg ctcctgctga agcgctgcac cgcctgcggg cgcgcgcacc
     1861 actacccgcg gccgatctgc ccgttctgcg ggagcgaccg caccgagtgg accgagtcgc
     1921 ccgggcgcgg cgtcatatac tccttcagcg tcatgcgccg ggccgcgccg ccctacgtca
     1981 tcgcctacgt gaccctggac gagggcgtca ccatgatgac gggcctggtg gagtgcgact
     2041 tcgacgcgct ccgcatcggc cagaaggtgc gcgtcgtgtt cgtgccgagc gacggcgggc
     2101 cgccggtgcc gatgttcagg ccggcctagc gccgggcccc ctcggcgccg gtcacggcag
     2161 cgggacgctc aggaagtacc gcggctcctt ccccttctgg cccgagatgt acggatcctg
     2221 ccatttgtcg tccgtgtcca ggaagccctt gccggtgggc ttcttcggcg gtgtgcggcc
     2281 cgcgagcagc gcgtcgcgaa gctgctgctc gagggcggcg cggaacttga agtggtcgta
     2341 gacgtccagc acgtggaccg cgtacgcgag cgcgagaggg cggttgccgc ggacgatgac
     2401 catgttctcg tcgttggcgt aggaggcctt gaagcccaga ttgtggctcc cggtgatcac
     2461 cgtgcaatcg gtcttcgaca gcgggtcgat gaccacgatc ttgtcgtgga tgatcgcgtg
     2521 gccggcgctc aggagctcct tctggaagtc gccgatgagg tcgttgatgg cggccgcgcg
     2581 aatcatgagc acgttgggcg cgccgttggg gctgaagatc gcgatctgcg ggatcttggg
     2641 cttcttcggg tccttcttct gtcctttcgg gggcgtgacg tagccgggca ccgccgtcgg
     2701 atcgctgatc gcgccggtca ccagcaggtt gggatcgagg cggccgaagt cgatgccggc
     2761 cgacaccacc gtgttgacgt cctcaccggc cgggtcgctg cgcccgggat tgaagacgag
     2821 gaagaagatc gcctccttcg ccttcttcat caggtcgaag accatcgcca gatcgggggg
     2881 cacggtgcgg ttctgcgacg tcccggtctt cggcgtattc ggcgagtacc acagatcgac
     2941 gtgggtcgcg ttcccgtcga gcggcaccgg ggtggacttg gcgtcggcgg tgcggagagg
     3001 ggtgccctgg tcgctcgcga ggggggcgct cagcggcttc ggcgtcggga gcttgtcggc
     3061 gtgtaggcgg ttccagtagt cgagatacgc ctgcgccacc gtcttcgact ccagcatgat
     3121 cgtgttgttg gtctgcgcgc acagcccggt cttcgtccag ttggtgctgc cggtccagac
     3181 cgccgtcggg gtgccgttct tcgcgatcag gaccgcgaac ttgttgtggc cgatgtgcgc
     3241 gctgttgttg aacatccgat tctgcagccg gtcgcccatg agggcgtgca gcttcttgcg
     3301 cgccccggtg ttggtcgtgt cccacttgac cggatcgccc gagcccttct tgggcggcgt
     3361 gctgcccgcc gtggagagaa tgatctggag acgctgctgg tgcttcacca gcagcccgag
     3421 cagctccttg tcctcgagct cgtagagagc cagacggacg gtggcgtcct ctcgatccgc
     3481 gcgctcgaac agcgagcgca gcgcgggtag cacgtcgccg gcgaggaact cgcggatcgg
     3541 atccccctcc ttcgcgatgt gcccttcgat gtccttcttg gtgggggcga cccccttggg
     3601 cgtcttgagc tgttttcgaa ggttctgggt ggagaggatc ccgttgttga aggccaccga
     3661 gatgtcgccg aagtcgccgg tcaccaggaa ctcgttggtc tggatggcgg ctccgcagac
     3721 gaacagcagg atcggcgtgc ccttgtactt gccgggctgc gcggtgggag acagggaaac
     3781 cggcgcccgg ccggggccgg gtaggccgac cggcacgatc tcgtacttcg ccttgaaatc
     3841 gggcggccgc gtggtcgcgg tgttccggct ccggcgcagc gtgagatcgc gccaggagaa
     3901 cttctggatc ggccacacgc tggtgtcctg cggctcccag tcgggattcg actgggtctc
     3961 gaaggcgacc cacgcgggaa ggattcggcg ttccacctcg tggccggtgg cgtcgaggtg
     4021 gatgcgcgtg atcatgaagc cgaggcatcc ggcgatcttt ccatcggtct gccaggcgag
     4081 ataggcgact tcgttgttgc agcacgcttg agcgagggtg agcttcgcca tcggagcctc
     4141 ctatcgattc ggatgggagt ggcaggatgc gccgggactc tagagggccg cccacgccat
     4201 gtcaaccgga agatgcccct attcggtgcc gttcggcgat cgtgcggcgg ggggagacga
     4261 ttctagaggc gcgccatcac ccggtccgcg aagtcctcga tcacgcgata ctgccggttg
     4321 agcggcgggt agagcatgat ctggctcagc ccctggctct ccagctcctt caactgctcc
     4381 acgatgcgct cgggcactcc ggt
//
LOCUS       DATMCV010000059         3119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_3453, whole genome shotgun sequence.
ACCESSION   DATMCV010000059 DATMCV010000000
VERSION     DATMCV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3453"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1072)
                     /locus_tag="VNU03_01490"
     CDS             complement(35..1072)
                     /locus_tag="VNU03_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWP73148.1"
                     /translation="MKLLRAAVWRLFHPRRTIKPTRDGWWCLFVAVGLGVAAINTGNN
                     LLYLLVSALLALIVVSGILSEQAMRGLALTGITPEEVYAGRPALLGATLANRKRWLTS
                     YSVTLELLATEARALESGSRFIYFRRIEAGTDRLATWEETLPRRGRRSLAGVRITTRF
                     PFGLFMKAGRPALTGEVLVFPAVRPVPPESLGRLGDAGDSAARRRGRGNDLYNLRAYR
                     PGDDPRFIHWRSSAKTEALMVREHEADTSHDSRLVLVGRGRAGTEALESGLSEAASLA
                     VALIRAGAGVELMGPDLFVPLGHGRPHLRRVLTALALYEPAPGSATAAQGDGEPSSGL
                     RRLREIRVPLA"
     gene            complement(1080..2021)
                     /locus_tag="VNU03_01495"
     CDS             complement(1080..2021)
                     /locus_tag="VNU03_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWP73149.1"
                     /translation="MGGSTEIIRRLEDSVGRALVGKPAVVRLAVIGLVARGHLLIEDV
                     PGVGKTTLAAALARSIGASFQRIQFTSDMLPSDVLGVSVWEPKTGDFVFKPGPLFTNI
                     VLADEINRTTPKTQSALLEAMNEAQVSLDHSTYPLPRPFMVLATQNPREYEGTFPLPE
                     SQLDRFLLRLRIGYPAAADEKAVLRGEAGPPPAGAAPVVLAEEVLSLQEAAERVRAEE
                     SVLDYLVALVTATRQSPLLALGVSPRGSLALLRAARARALADGRDYLLPDDIKELAGP
                     ALGHRVIVKASVPGVAAGGMDGEAAIAAILQDVPVPR"
     gene            2108..2932
                     /locus_tag="VNU03_01500"
     CDS             2108..2932
                     /locus_tag="VNU03_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HWP73150.1"
                     /translation="MTLEIPPSPRWPRDARAACAFTFDLDAETLWMARGVHEPVTLSQ
                     GRFGPVEALPRILALLRANEIRASFFIPAWVAEHHPDAVRGIAADGHEIGCHGDEHEK
                     VSDLPVEREEAILVKSLEVLTRIGGKRPLGYRAPAWQLSAETLGLLGRHGFDYSSNMM
                     DRLSPYLHPATGGRALVEIPVSWVLDDAPYFMFTGQRAMQPPGPVLQGWLTEFEGITE
                     ARGVTNFTFHPQIIGRPSRLACLRELIDHVRHTPRVWIASLAEISAHFRATAASAG"
     gene            complement(2940..>3119)
                     /locus_tag="VNU03_01505"
     CDS             complement(2940..>3119)
                     /locus_tag="VNU03_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73151.1"
                     /translation="EAKARDTVLAGSVSQIRDKLAKLQAAGVGMLFIPTFLLPTDRHG
                     ALDRFMSEIAPAFRS"
BASE COUNT          441 a         1120 c         1163 g          395 t
ORIGIN      
        1 ggtgacgagg cggagggcga ggagggccga catgtcaggc gagcggcacc cggatctcgc
       61 gcagccggcg cagaccggag gacggctcgc cgtccccctg ggccgcggtc gcggagcccg
      121 gggccggctc gtagagcgcg agcgcggtga gcacgcgacg gagatgcggg cggccgtggc
      181 cgagcgggac gaagagatcg ggccccatca gctcgacccc cgccccggcg cggatcaggg
      241 ccaccgcgag cgaggcggcc tcggagagcc cgctctcgag cgcctccgtg ccggcccggc
      301 ctcgccccac cagcacgagc cggctgtcgt ggctcgtgtc cgcctcgtgc tcgcgcacca
      361 tcaaggcctc ggtcttcgcg ctgctgcgcc agtggatgaa gcgcggatcg tcgcccggcc
      421 ggtaggcgcg gaggttgtac agatcgttgc ctcgcccgcg ccgccgcgcc gccgagtcgc
      481 ccgcatcgcc cagacgcccg agcgactcgg gcggcaccgg gcgcaccgcc gggaacacga
      541 gcacctcgcc ggtcagcgcg ggccggcccg ccttcatgaa caggccgaac gggaagcggg
      601 tggtgatgcg cacgccggcg agcgagcggc ggccgcggcg cgggagcgtc tcctcccagg
      661 tggcgaggcg gtccgtgccc gcctcgatgc gccggaagta gatgaagcgc gagcccgact
      721 cgagcgcgcg cgcctccgtc gcgagcagct cgagggtgac cgagtacgac gtgagccagc
      781 gcttgcggtt ggccagggtg gctccgagca gcgcggggcg ccccgcgtac acttcctccg
      841 gcgtgatgcc ggtgagggcc aggccgcgca tcgcctgctc agagaggatg ccggagacga
      901 cgatcagggc gagcagggcc gagaccagca ggtagagcag gttgttgccg gtgttgatcg
      961 cggccacccc gagccccacc gcgacgaaga ggcaccacca cccgtcccgg gtgggcttga
     1021 tggtgcgacg cgggtggaac agccgccaga ccgcggcccg caacagcttc accgcccggc
     1081 tacctcggaa cggggacgtc ctgcaggatg gcggcgatcg ccgcctcgcc gtccatcccc
     1141 ccggcggcca ctccggggac ggaggccttc acgatgaccc ggtgacccag cgcggggccg
     1201 gccagctcct tgatgtcgtc gggcagcagg tagtcgcgcc cgtcggcgag cgcacgggcg
     1261 cgcgcggcgc ggagcagggc cagcgatccg cgcgggctga cgccgagggc gagcagcggg
     1321 gactgccggg tggcggtcac cagcgcgacc aggtagtcga gcaccgactc ctcggcgcgc
     1381 acgcgctcgg ccgcttcctg gaggctcagc acctcctcgg ccagcaccac cggggccgcg
     1441 cccgcgggcg gcggcccggc ctcgccgcgc agcaccgcct tctcatccgc cgcggcggga
     1501 tagccgatgc ggaggcgaag caggaagcgg tcgagctgcg actcgggcag cgggaacgtg
     1561 ccctcgtact cgcgcgggtt ctgggtggcc agcaccatga acgggcgcgg cagcggatac
     1621 gtggagtgat cgagcgagac ctgcgcctcg ttcatcgcct cgagcagggc cgactgggtc
     1681 ttgggggtgg tgcggttgat ctcgtcggcg agcacgatgt tggtgaagag cgggcccggc
     1741 ttgaacacga agtcgccggt cttcggctcc cacaccgaga ccccgagcac gtcggacggc
     1801 agcatgtcgg aggtgaactg gatgcgctgg aacgaggccc cgatggagcg ggccagcgcg
     1861 gcggccagcg tggtcttgcc cacgccgggc acgtcctcga tcagcaggtg cccgcgcgcc
     1921 accaggccga tgaccgccag ccggaccacc gcgggcttgc ccaccagggc gcggccgacg
     1981 ctgtcctcga gacggcgaat gatctccgtc gagccgccca taggattcca ctatacagta
     2041 cgcggatttt tcgaccgccc gcgcgcgctg gcccgcgccg ggcgccatcg gctacaatgc
     2101 gggcgcgatg accctcgaga tcccgccctc cccgcgctgg ccccgtgacg cgcgcgccgc
     2161 gtgcgcgttc accttcgatc tcgacgcgga gaccctgtgg atggcgcgcg gagtgcacga
     2221 gccggtgacg ctctcgcagg gccgcttcgg tccggtggag gcgctgccgc gcatcctcgc
     2281 gctgctgcgg gccaacgaga tccgcgcgtc cttcttcatc ccggcgtggg tggccgagca
     2341 tcacccggac gcggtgcgcg gcatcgcggc cgacggccac gagatcggct gccacggcga
     2401 cgagcacgag aaggtcagcg atctgccggt cgagcgggag gaggcgatcc tggtcaagag
     2461 cctcgaggtg ctgacccgca tcggcgggaa gcggccgctc ggctaccgcg cgcccgcctg
     2521 gcagctctcc gccgagacgc tcggcctgct cggccgccac ggcttcgact actcgtcgaa
     2581 catgatggac cggctgtcgc cgtacctgca cccggcgacg ggcgggcgcg cgctcgtgga
     2641 gatcccggtc tcgtgggtgc tcgacgacgc gccgtacttc atgttcaccg ggcagcgggc
     2701 gatgcagccg ccgggaccgg tgctccaggg ctggctcacc gagttcgagg ggatcaccga
     2761 ggcgcgcggg gtgaccaact tcaccttcca cccccagatc atcggccggc cgtcgcggct
     2821 ggcctgcctg cgcgagctga tcgaccacgt gcgccacacc ccgcgggtgt ggatcgcctc
     2881 gctcgcggag atcagcgccc acttccgcgc gaccgccgct tccgcgggct gagctggagc
     2941 taggagcgga acgcgggcgc gatctcgctc atgaaccgat cgagcgcgcc gtgccgatcg
     3001 gtggggagca ggaacgtggg gatgaagagc atgcccaccc cggcggcctg gagcttggcg
     3061 agcttgtcgc ggatctggct cacgctgccg gccagcaccg tgtcgcgggc cttcgcctc
//
LOCUS       DATMCV010000060         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_868881, whole genome shotgun sequence.
ACCESSION   DATMCV010000060 DATMCV010000000
VERSION     DATMCV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868881"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VNU03_01510"
     CDS             <1..1146
                     /locus_tag="VNU03_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWP73152.1"
                     /translation="LGLSITLSETALTVLTLLWLWRLRDPAVRGAARWPLWQPVLAFS
                     AVSLVSALASGHPVTALGACKGLLLASALYVTADVLSGPADGHRFLSALLVVVTVAAV
                     VGLLQTVACPGPAADYGSPAWLYHRCYRARGFFSIYMTLAGVLSLVLLAMLPRLLPPG
                     AARRRLGVPWLMMLWGLIVTYTRGAWIGFAGGVLTVAATVRRGRWALVGGLLLLVAGA
                     LVSPYELRHRFLSMADPDEAGVRERIYMWKSGFAMWRERPILGVGPGGVKRDYVRYAR
                     EEALKKRTGHVHNTPLQILVERGVLGLAAWLWLWIAFYAHAIRRLRQLPAEAVAERAL
                     VVGGIAAITGFLIGGLSEYNFGDSEVVMVAWAIMALPWAAVKEEPSA"
     gene            1305..1754
                     /locus_tag="VNU03_01515"
     CDS             1305..1754
                     /locus_tag="VNU03_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWP73153.1"
                     /translation="MDAIQFLKQEHKKAKAGLKKVLKASPDTRGDLWNELQPELEAHE
                     QIEDACLYGPLARGAGKVDAKLAAWRAKHQKEVERVEGLVGQLENLDPAEAAWIAKVR
                     AIGTSLEKHIREEEQDIFPRIGKVWDRKQLAEAGAEMKEMKAEKLHA"
     gene            1747..>2504
                     /locus_tag="VNU03_01520"
     CDS             1747..>2504
                     /locus_tag="VNU03_01520"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HWP73154.1"
                     /translation="MPSRKAKANRPAGKAARSPKQEALDQFREDPEGQLLTTDQGLRV
                     NDDQNSLKAGPRGPSLLEDFILREKITHFDHERIPERVVHARGAAAHGYFQVERSLSR
                     YTRAAFLQDPSKRTPVFVRFSTVAGSRGSSDLARDVRGFAVKFYTEEGNFDLVGNNIP
                     VFFIQDAVKFPDLIHAVKPEPHNEMPQAASAHDTFWDFISLMPESMHMIMWAMSDRAI
                     PRSVRMMEGFGIHTFKLVDAKGASRFVKFHWKPLL"
BASE COUNT          373 a          860 c          874 g          397 t
ORIGIN      
        1 ctcggactgt ccatcacgtt gtccgagacg gcgctgaccg tgctgacgct cctgtggctc
       61 tggcgcctgc gcgatcccgc ggtgcgcggg gcggcgcgct ggccgctctg gcagcccgtg
      121 ctggccttca gcgcggtgag cctggtctcg gcgctcgcct ccgggcaccc ggtcacggcc
      181 ctgggcgcct gcaagggcct cctgctcgcc agcgccctct acgtgaccgc cgacgtgcta
      241 tccgggcccg cggacggcca ccggtttctc tcggcgctcc tggtggtcgt gacggtcgcg
      301 gcggtcgtcg gcctgctgca gacggtcgct tgccccgggc cggccgccga ctacggctcc
      361 ccggcctggc tctaccaccg ctgctaccgg gcccgcggct tcttcagcat ctacatgacc
      421 ctggccggcg tgctgtcgct cgtcctcctg gccatgctgc cgcggctcct tccccccggc
      481 gcggcccggc gccggctcgg ggtcccgtgg ctcatgatgc tgtgggggct gatcgtgacc
      541 tacacccggg gcgcgtggat cggcttcgcg ggcggggtcc tgacggtggc cgccaccgtc
      601 cggcgcgggc gctgggccct ggtcgggggt ctgctgctcc tggtcgccgg cgcgctggtg
      661 tcgccctatg agctgcgcca ccggttcctc agcatggccg atccggacga ggcaggggtg
      721 cgggagcgga tctacatgtg gaagagcggg tttgcgatgt ggcgggagcg tccgatcctg
      781 ggcgtcggcc cgggtggcgt caagcgtgac tacgtgcgct atgcgcggga ggaggcgctc
      841 aagaagcgca ccgggcacgt gcacaacacc ccgctgcaga tcctggtgga gcgcggggtt
      901 ctgggactgg ccgcgtggct ctggctctgg atcgccttct acgcccacgc gatccgccgc
      961 ctgcggcagc tgcccgcgga ggcggtcgcg gagcgcgccc tggtcgtcgg cggcatcgcg
     1021 gccatcaccg gcttcctgat cggcggcctc tccgaataca acttcggcga ctcggaagtg
     1081 gtcatggtcg cgtgggcgat catggcgctg ccctgggcgg cggtgaagga ggagccgagc
     1141 gcctagcaac gcttaccgag cacacccgtt gttaccgagt cgtttagcag tcgctcggag
     1201 ctttccgtcg cgtccgcctc cgccaacacc tcgctcatgc gcgcctctcg tcgtcgcgac
     1261 acaggggcac gctccttgcg tgaccatgcc cggggaggac gacaatggac gcgattcaat
     1321 tcctcaagca ggagcacaag aaggcgaagg ccgggctcaa gaaagtcctg aaggcctcgc
     1381 ccgacacgcg cggtgatctc tggaacgagc tccagcccga gctcgaggct cacgagcaga
     1441 tcgaggacgc ctgcctgtac ggaccgctcg cgcgcggggc gggcaaggtg gacgccaagc
     1501 tcgcggcctg gcgcgccaag caccagaagg aggtcgagcg ggtcgagggc ctggtcgggc
     1561 agctggaaaa cctggatccg gccgaggcgg cctggatcgc caaggtcagg gcgatcggca
     1621 ccagcctcga gaagcacatc cgggaggagg agcaggacat ctttccgcgg atcggcaagg
     1681 tctgggacag gaagcagctc gcggaagccg gggccgagat gaaggagatg aaggcggaga
     1741 agctgcatgc ctagcaggaa ggccaaggcg aatcgcccgg ccggcaaggc ggcccgctcc
     1801 ccgaagcagg aagccctcga ccaattccgc gaggatccgg aaggccagct gctcaccacc
     1861 gatcaaggcc tgcgcgtcaa cgacgaccag aactcgctca aggcgggccc ccgcgggccc
     1921 tccctcctgg aggacttcat cctccgggag aagatcaccc acttcgacca cgagcggatc
     1981 ccggagcggg tcgtccatgc ccggggcgcc gccgctcacg gctatttcca ggtcgagcgg
     2041 tccctgagcc ggtacaccag ggccgccttc ctccaggacc cgtcgaagcg gaccccggtc
     2101 ttcgtgcggt tctcgaccgt ggcgggctcg cgggggtcga gcgacctggc gcgcgacgtg
     2161 cgcggcttcg cggtgaagtt ctacaccgag gaaggcaact tcgatctggt cggcaacaac
     2221 atcccggtct tcttcatcca ggatgcggtg aagttccccg atctcatcca cgcggtcaag
     2281 ccggagccgc acaacgagat gccccaggcc gcgtccgcgc acgacacgtt ctgggatttc
     2341 atctcgctga tgcccgagtc catgcacatg atcatgtggg cgatgtccga ccgcgccatc
     2401 ccgcggagcg tccggatgat ggagggcttc ggcatccaca ccttcaagct ggtggacgcc
     2461 aagggggcct cacggttcgt gaagttccac tggaagccgc tcct
//
LOCUS       DATMCV010000061         3472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_800926, whole genome shotgun sequence.
ACCESSION   DATMCV010000061 DATMCV010000000
VERSION     DATMCV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800926"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VNU03_01525"
     CDS             complement(<1..980)
                     /locus_tag="VNU03_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73155.1"
                     /translation="MSQLVVSTECPTCSGPLDFSEGSNAIRCPSCGSHLLVTGRKQVL
                     SYWVKPRVRGEVAGAAARTGRLEARVARTQLFFVPFYRLTGHDFQWQDVPPRPEPENG
                     AFPTMVVGGSDRTVDRAELEIPIGSVLGWGADLLLGRHAGDVVRDMLGAPSKRDRSPE
                     AAILMAPARTLTTASGEATVQFLDRYVEKSFPAADLPGLGIYSLGIRAQALHVALFQR
                     EALAALGAVVPVQVDLAAAMAQGLSASGFEHVVYRQVLGRILSVIYFPLWVVELTQGT
                     ERWLTVVDAVAESVVQPRAPLALYDALARAATEESRTVGLRPLVCPNCGG"
     gene            complement(998..2056)
                     /locus_tag="VNU03_01530"
     CDS             complement(998..2056)
                     /locus_tag="VNU03_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWP73156.1"
                     /translation="MAPIMMEVIEWFDQTGTGIVQRYPEGGSGDIKMGAQLIVRENQA
                     AVFFRDGQGLDVFGPGRHTLSTQNLPILTRLLSLPWGFTSPFRAEVYFVNLKVFTNMR
                     WGTRDPVAFKDTQLGLIRLRAFGNFTMRVTQPLLFVNSLVGTQGSYTTDQIEEYLRDV
                     IVARLNDFLGEKVQSLIDLPPHYDEMAVALKTRLVDEFRKYGAELVDFYINRITPPEE
                     VERMIDERSGMGAVGDLDRFLKFKAAKAMGDAAQGGGGGVGNGAAAGVGVGVGAGLGM
                     MMPGMLAKSLGTEPAAAPARAVITCPGCQSAVASDSHFCPRCGRQLAATARCSRCQSE
                     LPGGARFCASCGQAVPGS"
     gene            complement(2069..2569)
                     /locus_tag="VNU03_01535"
     CDS             complement(2069..2569)
                     /locus_tag="VNU03_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73157.1"
                     /translation="MGVTVASRLERLVRFVPGVAGYQDREHSRATDKQVRMRLVQEIR
                     RLIQGLDDHKERLARSADLSALPRLDRLAGRLERQGRIVEFASRGYTGLFDLHHVDEE
                     TLDQLYAFDLGLFDALSVVRAKAEAVRAARADAAALETAIEHMTEALDDFGQLWDKRQ
                     RIVEAD"
     gene            complement(2713..>3472)
                     /locus_tag="VNU03_01540"
     CDS             complement(2713..>3472)
                     /locus_tag="VNU03_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWP73158.1"
                     /translation="FLWWPRCWTSAALRQVTRFDASLRGRARDFNWHNVIGIWCAPVL
                     LVITLTGVVMSYQWANDLLYRLTGSEPPPPAAGAERPATPAGRARGAEGRVREERPTR
                     PAEGRAPADLDALWERAERQVPGWVLISLRVPTRPDGPVTFVIQEPTGWHPTPRSQLT
                     LDPVTAEVVRWEPFAGQSLGRRLRSWVRPLHTGEAGGPVGQAIALVASGGGAVLVWTG
                     LALAWRRFRSWRRRMSAAGALSVSSQPGREVSAD"
BASE COUNT          520 a         1206 c         1283 g          463 t
ORIGIN      
        1 tcaccgccgc agttgggaca cacgagaggc cgcaggccga cggtgcgtga ctcctcggtg
       61 gcggcgcgcg cgagggcgtc atagagggcg agcggcgcgc ggggctgcac cacgctctcg
      121 gccaccgcgt cgaccacggt gagccatcgc tccgtcccct gcgtcagctc gacgacccag
      181 agcgggaagt agatcaccga gaggatgcgt cccaggacct ggcggtagac cacgtgctcg
      241 aagccgctgg ccgagaggcc ctgtgccatg gcggcggcca gatcgacctg caccgggacg
      301 acggcgccga gggcggccag cgcctcacgc tggaagagcg cgacgtgcag cgcctgggcg
      361 cggatgccga gcgagtagat cccgaggccc ggcaggtcgg cggccgggaa gctcttctcc
      421 acgtagcggt ccaggaactg cacggtcgcc tcgccgctcg cggtggtgag cgtgcgcgcg
      481 ggcgccatga ggatcgcggc ctcgggcgag cggtcgcgct tcgacggagc gcccagcatg
      541 tcgcgcacca cgtccccggc gtgccggccg agcagcaggt cggctcccca gccgagcacg
      601 gagccgatcg ggatctcgag ctcggcccga tcgaccgtgc ggtcgctgcc cccgacgacc
      661 atcgtgggga atgcgccgtt ctccggctcg ggcctgggcg ggacgtcctg ccactggaag
      721 tcgtggccgg tcagccgata gaagggcacg aagaagagct gggtgcgcgc gacgcgcgcc
      781 tcgagccgcc cggtgcgggc ggcggcgcct gccacctcgc cccggacccg gggcttcacc
      841 cagtagctca gcacctgctt gcgtccggtg accaggaggt gagagccgca gctcgggcag
      901 cggatcgcgt tggacccctc ggagaagtcg agggggccac tgcaggtggg gcactccgtc
      961 gagacgacga gctggctcat agccagccgg cccggcgtca gctaccgggg acggcctgac
     1021 cgcacgacgc gcagaagcgg gcgccgcccg gcagctcgga ctggcagcgc gagcaccgcg
     1081 cggtcgcggc cagctggcgg ccgcagcgcg ggcagaagtg gctgtcggag gccaccgccg
     1141 actggcagcc cgggcaggtg atgaccgccc tcgcgggcgc ggcggcgggc tcggtgccga
     1201 ggctcttggc gagcatgccc ggcatcatca tgccgagccc ggccccgact ccgaccccca
     1261 cgccagcggc cgcgccgttg ccaactccac caccgccgcc ctgcgcggcg tcgcccatgg
     1321 ccttggccgc cttgaacttg aggaagcgat cgaggtcgcc gaccgcgccc atgccggagc
     1381 gctcgtcgat catccgctcc acttcctcgg gcggggtgat gcggttgatg tagaagtcga
     1441 ccagctcggc gccgtacttc cggaactcgt ccacgaggcg ggtcttcagc gcgaccgcca
     1501 tctcgtcgta gtgcgggggc aggtcgatca ggctctgcac cttctcaccc aggaagtcgt
     1561 tgaggcgcgc gacgatgacg tcgcgcaggt actcctcgat ctggtcggtg gtgtaggagc
     1621 cctgggtgcc caccaggctg ttcacgaaga gcagcggctg ggtcacccgc atcgtgaagt
     1681 tgccgaacgc gcgaaggcgg atgagaccga gctgggtgtc cttgaaggcc accggatcgc
     1741 gcgtgcccca gcgcatgttg gtgaagacct tcaggttgac gaagtacacc tcggcccgga
     1801 aggggctcgt gaagccccac ggcagggaga gcagccgggt caggatcggc aggttctggg
     1861 tggagagggt gtggcggccc ggcccgaaga cgtcgagccc ctggccgtcg cggaagaaga
     1921 ccgcggcctg gttctcgcgc acgatgagct gcgcccccat cttgatgtca cccgagccgc
     1981 cctcgggata gcgctggacg atcccggtgc cggtctgatc gaaccactcg atgacttcca
     2041 tcatgatggg ggccatggcg tgtcctcctc agtccgcttc cacgatgcgc tgccgcttgt
     2101 cccagagctg cccgaagtcg tcgagggcct cggtcatgtg ctcgatcgcg gtctcgagcg
     2161 cggccgcgtc ggcgcgcgcg gcccggaccg cctcggcctt ggcgcggacc acgctcagcg
     2221 catcgaagag tcccaggtcg aaggcgtaga gctgatcgag cgtctcctcg tccacgtggt
     2281 ggagatcgaa gagcccggtg tacccgcggc tcgcgaactc gacgatgcgg ccctggcgct
     2341 cgaggcggcc ggcgagccga tcgagacggg gcagggcgga gagatcggcc gaccgcgcga
     2401 ggcgctcctt gtggtcatcg agcccctgga tcagccggcg gatctcctgg acgagccgca
     2461 tccgcacctg cttgtccgtg gctcgggagt gctcccgatc ctggtatccc gccactccgg
     2521 ggacgaagcg aacgagacgc tcgagcctgc ttgcgacggt gacgcccatg gaagcctcct
     2581 cttggtggac ccggaacgtg cccagaccag ggcgaggagt gtgcaagcca attgccagtg
     2641 gatgtgagtc tgaactactt gaatggaaag aggaggaggc ctctcgagcc cacctccggg
     2701 tggcggcccg tgtcagtctg ctgacacttc tcggccaggc tgcgaggaga cggagagggc
     2761 tcccgcggcg ctcatccgac ggcgccagct gcggaaccgt cgccaggcga gggcgaggcc
     2821 ggtccacacg aggacggcgc cgccgccgga ggccaccagc gcgatcgcct ggcccacagg
     2881 gccgccggcc tcgccggtat ggagcggccg cacccagctc cgcagccgcc gcccgaggct
     2941 ctggccggcg aagggctccc agcgcacgac ctcggccgtg accgggtcga gggtgagctg
     3001 agagcggggg gtcggatgcc acccggtcgg ctcctggatc acgaacgtga cggggccgtc
     3061 gggccgcgtc ggaacgcgca ggctgatcag cacccagccg ggcacctggc gctcggcgcg
     3121 ctcccagagc gcgtcgaggt ccgcgggcgc gcgcccctcg gccgggcgcg tgggccgctc
     3181 ctctcgcacc cggccctccg ccccgcgggc gcgtccggcc ggtgtcgcgg ggcgctccgc
     3241 ccccgcggcg ggcgggggcg gctcggagcc ggtcagccga tagagcaggt cgttggccca
     3301 ctggtaggac atgacgacgc cggtgagggt gatcacgagg agcaccggcg cgcaccagat
     3361 gccgatcacg ttgtgccagt tgaagtcgcg cgcccggcca cgcaggctcg catcgaagcg
     3421 agtgacctgc cggagcgccg cgctggtcca gcaccggggc caccagagga ag
//
LOCUS       DATMCV010000062         5247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_136705, whole genome shotgun sequence.
ACCESSION   DATMCV010000062 DATMCV010000000
VERSION     DATMCV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136705"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VNU03_01545"
     CDS             complement(<1..537)
                     /locus_tag="VNU03_01545"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWP73159.1"
                     /translation="MIGWSGVYHILATPFRDDGALDTEGLPRLVECALATGINGFTIL
                     GIAGEAHRLTDEERRRVVETVVKEVRGRVPVAVGVSASGTHLATTFARMAREHGADAL
                     MVAPPTGLRALDAVAEYYRTVAAATGLPIVLQDEPVTTQVSMPAPFIAQVCADIPVIE
                     AIKVEEPPTLPKITRLRAL"
     gene            complement(610..1584)
                     /locus_tag="VNU03_01550"
     CDS             complement(610..1584)
                     /locus_tag="VNU03_01550"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HWP73160.1"
                     /translation="MPDTALLRVKNLKKYFPIRGGLFSREVARVHAVDDVTFDLLKGE
                     TLGLVGESGCGKSTTGRCILRLIEPTAGEVWFDDKNVTTLDKRSLRHLRRDMQIIFQD
                     PYASLNPRMTVGSIIGEALVIHKLAKSKREREERVVHLLETVGLNADHLRRYPHEFSG
                     GQRQRIGIARALAVSPKLIVADEPVSALDVSIQAQVINLLEDLQKQFNLTYLFIAHDL
                     SVVEHISNRVAVMYLGKIVEIAPAKELYTNPKHPYTEALLSAVPIPDPAVKRKRILLE
                     GDVPSPIKPPSGCRFHTRCPVRVPSCSENEQVLKEISPGHWVACQVRA"
     gene            complement(1593..2570)
                     /locus_tag="VNU03_01555"
     CDS             complement(1593..2570)
                     /locus_tag="VNU03_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73161.1"
                     /translation="MADRLLDVKNLKTYFFTDEGVVRAVDGVDLYIEKGETLGVVGES
                     GCGKSVTALSIMKLIPQPPGRIVEGEIIYNGTNLVTLAPNKMRKIRGKEISMIFQEPM
                     TSLNPVFTCGEQIAEAIRLHEGLGRREAMAKTVDMLKLVHIPNAERRVKEYPHQLSGG
                     MRQRIMIAMALSCNPNLLIADEPTTALDVTIQAQILELLNELKSKLHMAVLLITHDMG
                     VIAETAQRVVVMYAAKVAEEAPVADLFKEPLHPYTQGLLRSIPRIDLAATARRRLETI
                     PGTVPTLRGDIAPGCRFAPRCPFVKSVCTEKDPVLKEVKPGHKVSCWLY"
     gene            complement(2605..3642)
                     /gene="ltaE"
                     /locus_tag="VNU03_01560"
     CDS             complement(2605..3642)
                     /gene="ltaE"
                     /locus_tag="VNU03_01560"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HWP73162.1"
                     /translation="MHDVVDLRSDTLTLPTQAMRDAMARAEVGDDVWEEDPTVRRLEE
                     IAARRTGKEAALFVSSGTQGNLVAVLAQTRAGQEVILDADSHVFNYEGAGAAVFGGVQ
                     TLPVKTARGFLTPEQVREHIRPSNIHIPTTGLVCLENTHNRHGGTCCTPEEIEAVAAA
                     AHAAGVAVHLDGARLFNAAVALRRPVADFARPVDSLTFCLSKGLGAPVGSLVCGSREL
                     VARARRVRKMLGGGMRQVGVLAAAGLIALESMVDRLAEDHANCRRLAEGVARLPRIGL
                     DLATVQTNIVIFRVDRDGGAADLVTGCLARKVKIHQTGPGTIRCVTHKDVDADDIARA
                     LEAFREITAAW"
     gene            complement(3649..4422)
                     /locus_tag="VNU03_01565"
     CDS             complement(3649..4422)
                     /locus_tag="VNU03_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWP73163.1"
                     /translation="MRLKFLSVPTMLLSLLLLLGGCETISDFFRQKPQALPPASELYA
                     EGESQLSKNRYEDARVAFRKVAERHPQSSYAPRARFLIGEAFYREGQFDKAIKEFEAF
                     MSFYPRHEISDLVQFRLAMSYYDQMKPVEQDQEITRKAMEAFKVLVREYPESRYAADA
                     LAKTDICRGRLAQKELWVAAYYINQGNPAAARQRLEKVIKEFPRTLVTPEALFRLGEV
                     YQVDGRTEEAQRMFRQVAEEYPYTDWGKRAAQRLQTATR"
     gene            4618..4884
                     /locus_tag="VNU03_01570"
     CDS             4618..4884
                     /locus_tag="VNU03_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008384793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73164.1"
                     /translation="MSTRIETEPRVPRRSLLLGCVGLALAVIPRPAVAQQKVAPKLVQ
                     YQEKPKGTQECDGCLHFVAPSSCKMVDGKINPKGWCALFAPKPK"
     gene            complement(4886..>5247)
                     /locus_tag="VNU03_01575"
     CDS             complement(4886..>5247)
                     /locus_tag="VNU03_01575"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HWP73165.1"
                     /translation="LGNVCFNPLSALTRATMAQIATDPEMRAVARAIMEEADSVARAL
                     GIELAIGVDQRLAGAEKVGAHKTSMLQDVESGRPLEVEAVVGAVVEIADLLRLPLPHL
                     RTVYACTRLLDQGLRSG"
BASE COUNT          770 a         1752 c         1877 g          848 t
ORIGIN      
        1 gagcgcgcgg agccgcgtga tcttgggcag ggtcggcggc tcctcgacct tgatcgcctc
       61 gatcaccggg atgtcggcgc acacctgggc gatgaacggg gccggcatgc tgacctgggt
      121 ggtgaccggc tcgtcctgca gcacgatcgg caggccggtg gccgccgcga cggtgcggta
      181 gtactcggcg accgcgtcca gggcccggag cccggtgggc ggggccacca tgagcgcgtc
      241 cgcgccgtgc tcgcgcgcca tgcgggcgaa ggtggtggcc aggtgggtgc cggaggcgga
      301 cacccccacc gccaccggca cgcggccgcg cacctccttg accacggtct ccaccacccg
      361 gcggcgctcc tcgtcggtga gacggtgggc ttcgcccgcg atgcccagga tggtgaagcc
      421 gttgatgccg gtggcgagcg cgcactcgac gaggcgcggc aggccctcgg tgtcgagggc
      481 gccgtcgtcg cggaacgggg tggcgaggat gtggtagacg ccggaccagc ctatcaaccc
      541 tgacccctct tcatatccgc tcgcctcgcc tgcggctgcg gctcgccaaa ctcgaactag
      601 ccgcgcccgt taggcgcgga cctggcaggc gacccagtgg ccgggcgaga tctccttcag
      661 gacctgctcg ttctcggagc aggaggggac ccggaccggg cagcgggtgt ggaagcggca
      721 gccggagggc ggcttgatcg ggctcggcac gtcgccctcc agcaggatgc gcttgcgctt
      781 caccgcgggg tcggggatcg gcaccgcgga gagcagggcc tcggtgtagg ggtgcttcgg
      841 attcgtgtag agctccttgg cgggagcgat ctccacgatc ttgccgaggt acatgaccgc
      901 cacccggttc gagatgtgct cgaccaccga gaggtcgtgg gcgatgaaga ggtaggtcag
      961 gttgaactgc ttctggaggt cttccagcag gttgatgacc tgggcctgga tggacacgtc
     1021 cagcgcggag accggctcgt cggccacgat cagcttcggg ctcaccgcca acgcgcgcgc
     1081 gatgccgatg cgctgacgct ggccgcccga gaactcgtgc gggtagcggc gcaggtggtc
     1141 ggcgttgagc ccgaccgtct cgagcaggtg caccacccgc tcctcccgct cgcgcttgga
     1201 cttcgccagc ttgtggatga cgagcgcctc tccgatgatc gagccgaccg tcatgcgcgg
     1261 gttgagcgac gcgtaggggt cctggaagat gatctgcatg tcgcggcgca ggtgccgcag
     1321 cgaccgcttg tcgagggtgg tcacgttctt gtcgtcgaac cagacctcgc ccgcggtagg
     1381 ctcgatcagg cgcaggatgc accgcccggt ggtggacttg ccgcagcccg actcgcccac
     1441 gaggcccagg gtctcgccct tcagcaggtc gaaggtcacg tcgtcgacgg cgtgcacccg
     1501 cgcgacctca cgggagaaga gcccgccgcg aatcgggaag tacttcttga ggttcttgac
     1561 gcgcaggagt gcggtgtcgg gcatggcgtc ctctagtaca gccagcagga caccttgtgt
     1621 cccggcttga cttccttgag caccggatcc ttctcggtgc acaccgattt cacgaagggg
     1681 cagcgcgggg cgaagcggca gccgggggcg atgtcgcccc ggagggtcgg caccgtgccc
     1741 gggatcgtct cgagccgccg ccgggcggtg gccgcgaggt cgatgcgcgg gatcgagcgc
     1801 agcaggccct gggtgtaggg gtggagcggc tccttgaaga ggtcggcgac cggcgcctcc
     1861 tccgctacct tcgccgcgta catcacgacc acccgctgcg cggtctccgc gatgacgccc
     1921 atgtcgtgcg tgatcaagag aacggccatg tgaagcttgg acttcagctc gttgagcagc
     1981 tcgaggatct gggcctggat cgtcacgtcg agcgcggtgg tgggctcgtc cgcgatcagg
     2041 aggttcggat tgcacgagag cgccatcgcg atcatgatgc gctgccgcat gccgcccgag
     2101 agctggtgcg ggtactcctt cacgcggcgc tccgcgttcg ggatgtgcac cagcttcagc
     2161 atgtcgaccg tcttggccat cgcctcgcgc cggccgagcc cctcgtggag gcggatggcc
     2221 tccgcgatct gctccccgca cgtgaacacc gggttgagcg aggtcatggg ctcctggaag
     2281 atcatcgaga tttccttgcc ccggatcttg cgcatcttgt tcggggccag cgtgaccagg
     2341 ttggtcccgt tgtagatgat ctcgccctcg acgatgcggc cgggcggctg gggaatcagc
     2401 ttcatgatgc tcagcgcggt caccgacttg ccgcagccgg actcgccgac cacgcccagg
     2461 gtctcgccct tctcgatgta gaggtcgacg ccgtcgacgg cccgcaccac accttcgtcg
     2521 gtgaagaagt acgttttcag gttcttgacg tccagcaggc gatcggccat agggtgcggc
     2581 ctcctctgac gggggtgcgg gcggctacca ggccgccgtg atctctcgga aggcctcgag
     2641 ggcccgcgcg atgtcgtcgg cgtcgacgtc cttgtgggta acacaccgga tggtgccggg
     2701 accggtctgg tggatcttca ccttccgggc caggcagccg gtgaccaggt cggcggcgcc
     2761 gccgtcccgg tccacccgga agatgacgat gttggtctgc acggtggcca ggtcgagtcc
     2821 gatccgcggc agccttgcca cgccctcggc gagccggcgg cagttcgcgt ggtcctcggc
     2881 cagccggtcg accatggact cgagcgcaat gagccccgcc gcggcgagca ccccgacctg
     2941 gcgcatgccg ccgccgagca tcttgcgcac ccgccgcgcg cgcgccacca gctcgcgcga
     3001 tccgcacacc agcgagccca ccggggcccc caggcccttg gagaggcaga aggtgagcga
     3061 gtcgaccggg cgcgcgaagt cggcgaccgg gcgccgcagc gccaccgccg cgttgaagag
     3121 ccgcgcgccg tccaggtgga ccgcgacgcc ggccgcgtgc gccgcggcgg ccaccgcctc
     3181 gatctcctcc ggcgtgcagc acgtcccgcc gtgccggttg tgggtgttct cgaggcagac
     3241 caggccggtg gtcgggatgt ggatgttgct gggccggatg tgctcgcgca cctgctcggg
     3301 agtgaggaag ccgcgcgcgg tcttgaccgg cagggtctgg actccgccga acaccgccgc
     3361 gcccgcgccc tcgtagttga agacgtggga gtccgcgtcg aggatgacct cctggccggc
     3421 gcgggtctgg gcgagcaccg cgaccaggtt gccctgggtc ccggaggaca cgaacagcgc
     3481 cgcctccttg ccggtgcggc gcgccgcgat ctcctccagc cggcgaaccg tcggatcctc
     3541 ttcccacacg tcgtcgccca cctcggcccg ggccatcgcg tcccgcatgg cctgggtggg
     3601 gagggtcagg gtgtccgagc gcagatcgac gacgtcgtgc atggacgatt acctcgtggc
     3661 ggtctggagt cgctgggccg cgcgcttgcc ccagtcggtg taggggtatt cttcggccac
     3721 ctgccggaac atccgctggg cttcctcggt ccggccgtcg acctggtaca cctcacccag
     3781 gcggaacagc gcctccggcg tcacgagcgt ccgcgggaac tccttgatga ccttctcgag
     3841 ccgctggcgc gccgcggccg ggttaccctg gttgatgtag taggccgcca cccacagctc
     3901 cttctgggcg aggcgcccgc ggcagatgtc ggtcttggcg agggcgtcgg ccgcgtagcg
     3961 gctctcgggg tactcgcgca ccagcacctt gaaggcctcc atcgccttcc gcgtgatctc
     4021 ctggtcctgc tccaccggct tcatctggtc gtagtagctc atcgccagcc ggaactggac
     4081 caggtcggag atctcgtggc gcggatagaa cgacatgaag gcctcgaact ccttgatggc
     4141 cttgtcgaac tgtccctcgc ggtagaacgc ctcgccgatc aggaagcgag cgcggggcgc
     4201 gtagctcgat tgcgggtgcc gctcggcgac cttgcgaaac gcgacccgcg cgtcctcgta
     4261 ccggttcttg ctgagctggc tctcgccttc cgcgtacagc tcggaagcgg gcggcagcgc
     4321 ctgcggcttc tggcgaaaga aatccgagat cgtctcgcac ccgcccaaga gcaggagcag
     4381 actcagcagc atcgtgggaa ccgagaggaa tttgaggcgc atcggccagg ggtcggccat
     4441 cgattggccc ccagttgtaa gggtggaagt ctggaaagtc aaggacatcg tacggggggt
     4501 aggggcccgg cggcaggagg gccgagggcc ccgccggtgg tacgctctgc tatgaatgat
     4561 ggggatctct gacggtggca tccatatgac agtggcatct ggccgaggga ggagaaagtg
     4621 agtacgagaa tcgagaccga gcccagggtg ccccggcggt ccctgctgct gggctgcgtc
     4681 gggctggcgc tggccgtcat cccccgcccg gcggtggcgc agcagaaggt ggcccccaag
     4741 ctggtccagt accaggagaa gcccaagggg acccaggagt gcgacggctg cctgcacttc
     4801 gtggccccga gcagctgcaa gatggtggac ggcaagatca accccaaggg ctggtgcgcc
     4861 ctcttcgccc ccaagccgaa gtagcctagc cgctccggag cccctggtcg aggaggcggg
     4921 tacaggcgta gacggtccgg aggtggggca gggggagccg gagaagatcg gcgatctcca
     4981 ccaccgcgcc gaccaccgcc tcgacctcca gcgggcggcc gctctcgacg tcctggagca
     5041 tcgacgtctt gtgggccccc accttctcgg cgccggccag ccgctggtcc acgccgatcg
     5101 ccagctcgat gccgagcgcg cgggcgaccg aatccgcttc ctccatgatc gcgcgggcga
     5161 ccgcccgcat ctcggggtcg gtggcgatct gggccatggt cgcgcgggtg agcgcgctca
     5221 gcggattgaa gcagacgttg ccgagca
//
LOCUS       DATMCV010000063         5484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_736167, whole genome shotgun sequence.
ACCESSION   DATMCV010000063 DATMCV010000000
VERSION     DATMCV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736167"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VNU03_01580"
     CDS             complement(<1..414)
                     /locus_tag="VNU03_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73166.1"
                     /translation="MSPRPAGRRRAPRAAAAAPARLLPRDTNTVEARAGGHAVTLTNL
                     RKSFWPDEGRTKRDLLQYYFDLAPVLLPHLKDRAMVMKRYPNGAGGPFFFMKRAPSPR
                     PDWIETCAIEHASKSVIDFPIIQDVAALLWVVNLGC"
     gene            complement(411..1049)
                     /locus_tag="VNU03_01585"
     CDS             complement(411..1049)
                     /locus_tag="VNU03_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73167.1"
                     /translation="MSAPPRIFLLSPAHCGGERAALLFRKEASFDLARRVRKPPGVPL
                     GEVFSFLSGLYFRGKLVYAEAFAAPPAGLAGVYVITPSEGLRPADELVDLARLRGFAQ
                     VDIDAADARYHRPLRRDAARVAAELGPAGEAVLLGSVATGKYVDPLTEALGDRLRFPA
                     DFVGRGDMSRGGLLLRCVRAGRELAYVPVTGAARRGGRPARLERLLRLERLP"
     gene            complement(1046..2797)
                     /locus_tag="VNU03_01590"
     CDS             complement(1046..2797)
                     /locus_tag="VNU03_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWP73168.1"
                     /translation="MDRIAIARALREMADLLALTGRDPFRARAYERGARVLEGLSEPD
                     FERVVAKGRLTTLAGIGRGLAAVIGDLRGTGRSETLEKVRGSLPAGARELGRVPSLGL
                     RKIQALHAALGVDTVEALRAACEAGRVRTVKGFGEKTEQRILESIRALDAASSDDAPA
                     PSRVLLPHALDVAERVLAHVRAMPGVTAAEVAGDLRRWTETVDRLVVVLASPRPGAAL
                     DAALLSPLLVSRGTRDGLVARTTLVTGLPLELRATAPDRWAATLLAATGSDGHLRDLA
                     PLAQARKVDLEHAPGQDEAQLYRRLGLPYIPPELREGVGEVEAAQAGTLPDDLVTPDD
                     VQGLVHCHTVYSDGRHTIEQMARAADALGMRYLTITDHSPSAFYAKGLAVDRLRAQWD
                     ELARVQERVAVRLLRGTESDILADGALDYPDRILEQLDVIIASVHARLRMDAARMTER
                     IVRAMRHPCFKIWGHALGRLLLSRPPIEAHVEAILDVIAESLAAIEINGDPHRLDLEP
                     RFLRAARERSIQFVISTDAHATGELRNVRYGVAMARRGWVRRGEVLNTWDVDAFVRAV
                     SPTGAHRARQERQPVGR"
     gene            2906..3160
                     /locus_tag="VNU03_01595"
     CDS             2906..3160
                     /locus_tag="VNU03_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73169.1"
                     /translation="MFGRLLIAAITGLVVWKYREPITEYVRGNAGPARETVDELLRTV
                     QGRSESFLDQAKARISSRLESTRDKVRGGALEPGRGTPTG"
     gene            complement(3194..5230)
                     /locus_tag="VNU03_01600"
     CDS             complement(3194..5230)
                     /locus_tag="VNU03_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP73170.1"
                     /translation="MSAVTVDPITLEVLRETFSSIVREMRVTLVRTAYSSVLYEGEDF
                     SCVLMDGDAQLVSMSKGQDHPLHIVPISWSMRSVREKFGDDIHPGDIFLHNDPYTGGT
                     HLNDVAMIYPLFADDRLFVFPVVRAHWGDVGGMSPGSLTGDATEIFQEGVRIPPIRIV
                     DRGSPNRAALDLIFANMRGPRERIGDFDAMIGTCRKAAERVEALAARYGAPVVRAAVV
                     ELMDRAEQRMRRAIGALPDGEYSYESHLESGRVRLEPLSIRARVTVAGETITVDLTGT
                     SPQTAAPTNVGPAMAPTGAFTIIKSFLDPGTDVNSGAFRPLHVITPRGTIVNADPPAP
                     CGGMVEVKYCVETAVMGALAQALDGRVAGDLKGGGNHCYVGGPDPRTGETFIFYEYPA
                     GGTGGFDGGDGSNTVRTWTESDMTTLQPIEAVEQLYPVRIESTRLREDSGGPGRWRGG
                     LGLIREVRVLVASSRLSVLAEKAVLPPFGVCGGAAGATNRFWVRRGDRPIQPSPLPGK
                     VGGFPIQPGDVVLMESSGGGGFGDPLERDPARVAADLAQGYVTPAGAEREYGVVWRSG
                     RVDEVATRARRESLRAARPRVRLTPVSDLDADRGRLVRIDTETAGRLGVGPGAIVELV
                     NPRGAPLRAWVAALLPGNGGRAEIAPGAIAMLSGGDRDLVEVRAVHSGVLDLPA"
     gene            complement(5227..>5484)
                     /locus_tag="VNU03_01605"
     CDS             complement(5227..>5484)
                     /locus_tag="VNU03_01605"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73171.1"
                     /translation="PKPAAERHTAAGATLEAALVERRPVWFAGRAHDCPVWDRDRLPE
                     GAQVEGPAIVEEFGATTVVPPGWRGTLDGHGNLRFEREARA"
BASE COUNT          764 a         1938 c         2094 g          688 t
ORIGIN      
        1 gcagccgagg ttgaccaccc agaggagcgc ggcgacgtcc tggatgatcg ggaagtcgat
       61 cacgctcttc gacgcgtgct cgatggcgca ggtctcgatc cagtccggcc ggggagacgg
      121 ggcgcgcttc atgaagaaga acgggccgcc cgccccgttc gggtaccgct tcatgaccat
      181 cgcccgatcc ttcagatggg gcaggagcac cggcgccagg tcgaagtagt actggaggag
      241 gtctctcttg gtgcggccct cgtcgggcca gaacgacttg cgcaggttgg tgagcgtgac
      301 cgcgtggccg ccggcccgcg cctcgacggt attcgtatcg cgcgggagca ggcgggcggg
      361 ggccgcggcg gccgcgcgcg gggcccgccg cctgccggcc ggccgcgggc tcatggcagc
      421 cgctccagcc ggagcaggcg ctcgaggcgg gcgggacggc cgccgcggcg cgccgcgccg
      481 gtcaccggca cgtaggcgag ctcgcgcccc gcccgcacgc agcggagcag cagcccgccg
      541 cggctcatgt cgccccggcc gacgaagtcc gccgggaagc ggagccgatc cccaagcgcc
      601 tcggtgaggg ggtccacgta cttgccggtg gcgacgctgc cgagcagcac cgcctcgcct
      661 gccggcccga gctccgccgc gacacgggcg gcgtcgcggc ggagcgggcg atggtaacgg
      721 gcgtcggcgg cgtcgatgtc cacctgggcg aagccgcgca gccgcgccag gtccaccagc
      781 tcgtcggccg ggcgcagccc ctcgctgggc gtgatcacgt acaccccggc gaggccggcc
      841 ggcggtgccg cgaaggcctc cgcgtacacg agcttgcccc ggaagtagag gccgctcagg
      901 aatgagaaga cctcgccgag cgggacaccc ggcggcttac gcacgcgacg ggccaggtcg
      961 aagctggcct ctttccggaa gaggagggcg gccctctccc ctccgcaatg ggcgggggag
     1021 agcaggaaga tccggggcgg cgcgctcatc ggccgaccgg ctgtcgctcc tggcgggccc
     1081 ggtgagcgcc ggtcggggag accgcgcgca cgaaggcgtc gacgtcccag gtgttgagca
     1141 cctcgccgcg gcgcacccag ccgcggcggg ccatcgccac gccgtagcgg acgttgcgca
     1201 gctcgccggt ggcgtgcgcg tccgtcgaga tgacgaactg gatggagcgc tcccgggcgg
     1261 cccggaggaa gcgcggctcg aggtcgagcc ggtgcgggtc gccgttgatc tcgatggccg
     1321 cgagcgactc cgcgatcacg tcgaggatcg cctcgacgtg ggcctcgatc ggcgggcggg
     1381 agaggagcag gcggcccagg gcgtggcccc agatcttgaa gcacgggtgc cgcatggcgc
     1441 ggacgatgcg ctcggtcatg cgcgccgcgt ccatgcggag gcgggcgtgg acgctggcga
     1501 tgatcacgtc gagctgctcg aggatccggt ccgggtagtc gagcgcgccg tcggccagga
     1561 tgtcggactc ggtgccgcgc aggaggcgca ctgccacccg ctcctgcacc cgggccagct
     1621 cgtcccactg cgcgcgcaac cgatccaccg cgaggccctt ggcgtagaac gcgctcggcg
     1681 agtggtccgt gatcgtgaga tagcgcatgc cgagcgcgtc ggcggcgcgc gccatctgct
     1741 cgatggtgtg ccggccgtcg gagtagacgg tgtggcagtg aacgagcccc tgcacgtcgt
     1801 ccggggtgac cagatcgtcg gggagggtgc cggcctgcgc ggcctccacc tcgcccacac
     1861 cttcgcgcag ctccggcggg atgtagggca ggccgagccg gcggtagagc tgcgcctcgt
     1921 cctgtccggg ggcgtgctcg aggtcgacct tccgggcctg ggccagcggc gcgagatcgc
     1981 ggaggtggcc gtcggacccg gtggcggcca ggagcgtggc cgcccagcgg tcgggcgcgg
     2041 tggcccgcag ctcgagcggg aggccggtca cgagcgtggt gcgcgcgacc agcccgtcgc
     2101 gcgtgccgcg ggaaaccagc aggggggaca aaagcgcggc gtcgagggcg gccccgggcc
     2161 tgggagaggc gaggaccacc accagccggt ccaccgtctc cgtccaccgg cgcaggtccc
     2221 cggcgacctc ggccgcggtc acgcccggca tcgcgcgcac gtgggcgagg acccgctccg
     2281 ccacgtcgag ggcgtggggc aggagcacgc gactcggcgc gggcgcgtcg tccgatgacg
     2341 cggcatcgag cgcgcggatg gactcgagga tgcgctgctc ggtcttctcg ccgaatccct
     2401 tgaccgtccg cacccgcccg gcctcgcagg cggcgcgcag cgcctcgacc gtgtcgacgc
     2461 ccagcgcggc gtggagcgcc tggatcttcc gcaggccgag gctcgggacg cggccgagct
     2521 cccgcgcgcc ggcggggagc gagccgcgca ccttctcgag agtctccgag cgaccggtgc
     2581 cacgcagatc gccgatcacc gcggccaggc cccggccgat gccggcgagc gtggtcaggc
     2641 ggcccttggc cacgacccgc tcgaagtcgg gctcgctcag gccctcgagc acgcgggcgc
     2701 cccgctcata ggcccgcgcc cggaacgggt cgcgcccggt gagggcgagc aggtcggcca
     2761 tctcgcggag agcacgcgcg attgcgatgc gatccattcg tcccattgga gcaaggcacg
     2821 tgccggggtg caacaactcg gcgcgtcgtg gcacgcgaga tgctgaactc agcgtggagc
     2881 aaaatacgcc gaggaggatc cctacatgtt cggaagactc ttgattgctg cgatcaccgg
     2941 gctggtggtg tggaagtacc gtgaaccgat caccgagtac gtcagaggca atgcgggacc
     3001 ggccagagaa acggtcgatg agctgctgcg gacggtgcag ggcaggtccg agagcttcct
     3061 cgaccaggcg aaggcgcgca tctcctcgcg gctcgagagc actcgcgaca aggtccgtgg
     3121 aggggcgctc gagcccggga gaggaacacc taccgggtag ttattacttt cgggagccta
     3181 cgccgggcga tccctacgcc gggagatcaa ggacgcccga gtgcacggcg cgcacctcga
     3241 ccaggtcgcg atcgccaccg gacagcatgg cgatcgcgcc cggggcgatc tcggcacgcc
     3301 caccgttgcc gggcaggagc gcggcgaccc acgcgcggag gggcgccccc cgcgggttga
     3361 ccagctcgac gatggcgccc ggcccgacgc ccagtcgtcc ggcggtctcc gtatcgatcc
     3421 gcacgaggcg gccccgatcc gcgtcgagat cggagaccgg ggtgaggcga acgcgcggcc
     3481 gcgccgcgcg taggctctcg cggcgggcgc gcgtggcgac ctcgtccacg cggccgctgc
     3541 gccagaccac cccgtactcg cgctccgccc ccgccggcgt cacgtagccc tgggccagat
     3601 ccgcggccac gcgcgcggga tcgcgctcga gtggatcgcc gaagccgccg ccgccggagc
     3661 tctccatcag gaccacgtcg ccgggctgga tcgggaagcc gccgaccttg ccgggcagcg
     3721 gggagggctg gatcggccgg tcgccgcgac gcacccagaa ccggttggtc gcgccggccg
     3781 cgccgccgca gacgccgaag ggcgggagca ccgccttctc ggccagcacc gagagacggc
     3841 tcgacgccac cagcacgcgg acctcgcgga tgagaccgag gccgccacgc cagcgaccgg
     3901 gcccgcccga atcctcgcgg aggcgggtgg attcgatgcg caccgggtag agctgctcga
     3961 ccgcctcgat cggctggagc gtggtcatgt cgctctcggt ccacgtgcgg acggtgttgc
     4021 tgccgtcgcc gccgtcgaag ccgccggtgc cgccggccgg gtactcgtag aagatgaagg
     4081 tctcgccggt gcgcgggtcg ggcccgccga cgtagcagtg gttgccgccg cccttgagat
     4141 cgccggccac ccggccgtcc agggcctggg cgagcgcgcc catcaccgcg gtctccacgc
     4201 agtacttcac ctcgaccatg cccccgcacg gcgcgggcgg gtccgcgttg acgatcgtgc
     4261 cgcgcggggt gatgacgtgc aggggccgga acgcccccga gttcacgtcg gtgcccgggt
     4321 cgaggaagga cttgatgatc gtgaaggcgc cggtgggcgc catcgccggg cccacgttgg
     4381 tgggggccgc ggtctggggc gacgtgccgg tcagatcgac ggtgatggtc tcgcccgcga
     4441 cggtgacccg ggcccggatg ctgagcggct ccaggcgcac gcgcccgctc tcgagatggg
     4501 actcgtacga gtactcgccg tcgggcagcg cgccgatcgc gcggcgcatg cgctgctcgg
     4561 cccggtccat cagctcgacc accgcggcgc gcaccaccgg ggcgccgtag cgggcggcga
     4621 gggcctccac ccgctcggcc gccttgcggc aggtaccgat catcgcgtcg aagtcgccga
     4681 tgcgctcccg gggcccgcgc atgttggcga agatcaggtc gagcgcggcc cggttcggcg
     4741 agccccggtc cacgatccgg atgggcggga tgcggacgcc ctcctggaag atctcggtgg
     4801 cgtcgccggt gaggctgccc gggctcatgc cgcccacgtc gccccagtgc gcgcgcacca
     4861 ccgggaagac gaagaggcgg tcgtccgcga acaggggata gatcatcgcc acgtcgttca
     4921 gatgggtgcc gccggtgtag gggtcgttgt gcaggaagat gtcgcccggg tggatgtcgt
     4981 cgccgaactt ctcgcgcacc gatcgcatcg accagctgat cgggacgatg tggagggggt
     5041 ggtcctggcc cttggacatg gagacgagct gggcgtcgcc gtccatgagc acgcaggaga
     5101 agtcctcgcc ctcgtagagg acggaggagt acgcggtccg cacgagggtc acgcgcatct
     5161 cgcgcacgat cgacgagaac gtctcccgca ggacctcgag cgtgatcggg tccaccgtga
     5221 ccgcgctcat gcccgggcct cccgctcgaa gcgcaggttg ccgtggccgt ccagcgtgcc
     5281 gcgccagccg ggcggcacga cggtggtcgc gccgaactcc tcgacgatgg ccgggccctc
     5341 cacctgggcg ccctcgggga gccggtcccg gtcccagacc gggcagtcgt gggcgcggcc
     5401 cgcgaaccag accgggcgcc gctcgaccag ggcggcctcc agggtcgcgc cggccgcggt
     5461 gtggcgctcc gcggcgggct tcgg
//
LOCUS       DATMCV010000064         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_736215, whole genome shotgun sequence.
ACCESSION   DATMCV010000064 DATMCV010000000
VERSION     DATMCV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736215"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VNU03_01610"
     CDS             complement(<1..1051)
                     /locus_tag="VNU03_01610"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWP73172.1"
                     /translation="MSMRAGGAAVALALLVGPAHGADLTLGPRLEYKHVSRGHEFHVS
                     GAAVAARADGRPVVAWAAQEGHANQLYVLLPDETTPRRVNPDGLGVEALHHPPRLAIT
                     PGGGPMYVSWSSEKPKPEGTLFASDLRLSRSLDGGRTFEAPLRVNEDRPISHSFDGLA
                     LAADGTVLVTWIDGHEGRPDPATWLARVVDGGTRVESIRKVGDDTCVCCRVDAAVGPA
                     DSVALAWRRVFPGDIRDMVMAVSRDGGRTFGDPTLVSADRWKINACPHRGGAVGWDGR
                     GRIYMSWYTEGTDIRPDLRFAVSTDGRRFGPARRLHTSATSIPDNARMAVDPAGRAVV
                     VWEESTAVRRRILLRY"
     gene            complement(1048..>2191)
                     /locus_tag="VNU03_01615"
     CDS             complement(1048..>2191)
                     /locus_tag="VNU03_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWP73173.1"
                     /translation="FAIIDDTTYSYGTELRYVLTAPLVGMANRFTAGLQFFGTRQIDV
                     DFANVVGNRGAKTKDQFNIANTVAGYLEDQLDLTRTFTAIVGGRGVYTTREVRDRFLS
                     DGNQSDAVDFAAASPRAGFVWKPAPTVQVFGNASHAYEPPLLLEITAPGQLAGNLGQL
                     AAQKSWQFEVGTRGNVGERLAWDVSVYDIELWDEIQNVNVQPFPGAPFTIPRFRNIDR
                     SRHTGVEVGADVLLVKDVLSRLGLGRVGDTLRARAAYTWSRFKFVDDVNFGNHDLPGA
                     PPHFIQAELRYDHGSGFWMAPGLDIVPQGYFVNSENNARNHAYTLVNFRTGFEHKPTG
                     LGVFFEARNLTDQNYAAAVAVDDANRRFFFPGDGRSFYGGISWRWR"
BASE COUNT          314 a          754 c          818 g          305 t
ORIGIN      
        1 tgtaccgcag caggatgcgc cggcgcaccg cggtggactc ctcccacacc accaccgcgc
       61 ggccggcggg gtccaccgcc atccgcgcgt tgtcgggaat ggaggtggcc gaggtgtgca
      121 gccgccgggc gggaccgaag cgccggccat cggtcgagac cgcgaagcgc aggtcgggcc
      181 ggatgtcggt gccctcggtg taccagctca tgtagatgcg cccgcggcca tcccagccga
      241 ccgcgccccc gcgatgagga cacgcgttga tcttccagcg atcggcgctc accagggtcg
      301 gatcgccgaa ggtgcgcccg ccgtcgcgcg agaccgccat caccatgtcg cggatgtcgc
      361 cgggaaagac ccggcgccag gccagcgcca ccgagtcggc cggcccgacc gccgcgtcga
      421 cccggcagca cacgcaggtg tcgtcgccga cctttcggat cgactcgacg cgggtgcccc
      481 cgtcgaccac gcgggcgagc caggtggccg ggtcgggccg cccctcgtgg ccgtcgatcc
      541 aggtcacgag cacggtgccg tcggcggcca gcgcgagccc gtcgaaggag tgcgagatcg
      601 ggcggtcctc gttcacgcgc agcggcgcct cgaacgtccg gccgccgtcg agggagcggg
      661 agaggcgcag gtcggaggcg aagagcgtgc cctcgggctt gggcttctcc gaggaccacg
      721 agacgtacat cggcccgccg cccggcgtga tcgcgagccg cggcggatgg tggagggcct
      781 ccacgccgag gccgtcggga ttcacccgcc gaggcgtcgt ctcatcgggc aggaggacat
      841 agagctggtt ggcgtggccc tcctgcgcgg cccacgccac caccggccgg ccgtcggccc
      901 gcgccgccac cgcggccccg gagacgtgga actcgtgtcc ccggctcacg tgcttgtact
      961 cgaggcgcgg gcccagggtc aggtccgcgc cgtgcgcggg gccgaccagg agcgccagcg
     1021 cgaccgccgc accgccggct cgcatgctca tcgccacctc caggagatgc cgccgtagaa
     1081 ggaccggccg tcgccgggga agaagaagcg ccggttcgcg tcgtccaccg cgaccgccgc
     1141 cgcatagttc tggtcggtca ggttgcgggc ctcgaagaag acgccgaggc cggtcggctt
     1201 gtgctcgaat ccggtccgga agttgaccag ggtgtaggcg tgattgcgcg cgttgttctc
     1261 gctgttgacg aagtagccct gcggcacgat gtcgagcccc ggcgccatcc agaagccgga
     1321 gccgtgatcg tagcgcagct cggcctggat gaagtggggc ggcgcgccgg gcaggtcgtg
     1381 gttgccgaag ttgacgtcgt ccacgaactt gaagcgcgac caggtgtagg cggcgcgggc
     1441 gcgcagggtg tcgccgaccc ggccgaggcc gagccgcgag agcacgtcct tcacgagcag
     1501 gacgtcggcc ccgacctcga ccccggtgtg gcgcgagcgg tcgatgttgc ggaagcgcgg
     1561 gatggtgaac ggtgcgcccg ggaagggctg gacgttgacg ttctggatct cgtcccacag
     1621 ctcgatgtcg tagaccgaca cgtcccacgc caggcgctcg cccacgttgc cccgcgtgcc
     1681 gacctcgaac tgccacgact tctgcgccgc gagctggccc aggttgcccg cgagctggcc
     1741 gggggcggtg atctcgagga ggagcggcgg ctcgtaggcg tggctcgcgt tgccgaagac
     1801 ctgcaccgtc ggcgcgggct tccacacgaa gccggcccgc ggcgaggcgg ccgcgaagtc
     1861 caccgcgtcc gactggttgc cgtcggagag gaagcggtcg cggacctcgc gggtggtgta
     1921 gactccgcgg ccgcccacga tcgcggtgaa ggtgcgggtc aggtcgagct ggtcctccag
     1981 gtagccggcc accgtgttcg cgatgttgaa ctgatccttg gtcttggcgc cccggttgcc
     2041 gaccacgttg gcgaagtcga cgtcgatctg ccgggtgccg aagaactgca ggcccgcggt
     2101 gaaccggttg gccattccca cgagcggcgc ggtcagcacg tagcgcagct cggtgccgta
     2161 gctgtaggtg gtgtcgtcga tgatcgcgaa g
//
LOCUS       DATMCV010000065         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_136940, whole genome shotgun sequence.
ACCESSION   DATMCV010000065 DATMCV010000000
VERSION     DATMCV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136940"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VNU03_01620"
     CDS             <1..384
                     /locus_tag="VNU03_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HWP73174.1"
                     /translation="VEFTVAPVFPTAGEPRGRHEWLVEFRVPPTEPEDFVRILDEALA
                     GLNTDYRTKRAGSVGMMAPCMTVLPSGTFHRWMRGTGRLGDQHKVPRVMNDRGVADAL
                     LAHDGGESRRVDMRATERHASVANM"
     gene            421..984
                     /locus_tag="VNU03_01625"
     CDS             421..984
                     /locus_tag="VNU03_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HWP73175.1"
                     /translation="MVATRTRWRIGVLDSNPHSRAEVSRLIESGGATVVVDAPPRSES
                     VALLRRLAPDAVVLAAEDAMRDGDPVGGVLPPDLPAPVVLVSGRATATVLGWARIAGV
                     MGVLLRPLRPEELRPTLEIAIARFRELRRLRRVLADRPVVEAAKARLMSRDGLSEAAA
                     FGWLRRRAMEQRMRMGEVARAVLGCDA"
     gene            1002..1586
                     /locus_tag="VNU03_01630"
     CDS             1002..1586
                     /locus_tag="VNU03_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWP73176.1"
                     /translation="MACRDLNIAGLLVKRGAMHWGDEPVSALDHALQCAARARAARAA
                     DEVVLAALLHDVGHLISDSEETEFSHHGLWAARFLRPFVPARVAWLVEYHVLAKRYLC
                     SVDRVYAESLSLGSTRSWIRQGGVLDLDARREMERQPWLGEALALRRWDEEAKHPGVV
                     VPALGDYRELLEACFGRQLWEAAAVGGHDLAASL"
     gene            1641..>2119
                     /locus_tag="VNU03_01635"
     CDS             1641..>2119
                     /locus_tag="VNU03_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016978033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWP73177.1"
                     /translation="MNRLIVWALAVGLTVVGPSVAGSQDGLRLTGSGATFPFPLYSTW
                     FKAFSSKQKVVSVDYQGKGSGAGIQDLLNHTVDFAASDAAMTDEDMAKVAGGVQLLPM
                     TAGEIVLAYNLPGNPKALKLPRDVYAGIFLGKVTKWSDPQLKAANPGVTLPDQDITVV
                     "
BASE COUNT          291 a          699 c          817 g          312 t
ORIGIN      
        1 gtcgagttca cggtggcccc cgtgttcccg accgcggggg agccgcgcgg ccggcacgag
       61 tggctcgtcg agttccgggt gccgcccacc gagccggaag acttcgtgcg cattctcgac
      121 gaggcgctcg cggggctcaa caccgactac cgcaccaagc gcgcaggctc ggtgggcatg
      181 atggccccgt gcatgacggt gctgccgtcc ggcaccttcc atcgctggat gcggggaacg
      241 ggacggctcg gcgatcagca caaggtgccg cgcgtgatga acgaccgtgg cgtggccgac
      301 gcgctgctcg cgcacgacgg cggcgagagc cgtcgcgtgg acatgcgcgc gaccgagcgc
      361 cacgcctccg tagcgaacat gtgatttgac cccacgaggg cttgcgccga ccctgccctc
      421 atggtcgcga cccgcacgcg ctggcgcatc ggagtcctcg attcgaatcc tcactcgcgc
      481 gcggaggtct cgcggctgat cgagtccggc ggcgcgacgg tggtggtcga cgcgccgccc
      541 cgttccgaga gcgtggcgct gttgcggcgc ttggcgccgg acgcggtggt gctcgcggcc
      601 gaggacgcga tgcgagacgg cgacccggtc ggcggcgtcc tgcctcccga cctcccggcc
      661 ccggtggtcc tggtgtcggg ccgggccacc gccaccgtgc tcgggtgggc gcggatcgcg
      721 ggcgtgatgg gggtgctgct ccggccgctc cgcccggaag agctgcgccc gaccctcgag
      781 atcgcgatcg cgcgcttccg ggagctgcgg cgtctgcggc gagtcctcgc ggatcgaccg
      841 gtggtggagg cggccaaggc gcggctgatg agtcgcgacg gcctctcgga agccgccgcg
      901 ttcggatggc tgcggcgtcg ggccatggag cagcggatgc gcatgggcga ggtggcgcgc
      961 gcggtgctgg gttgcgacgc gtgacgccgg gggaggacga catggcgtgc cgggacctga
     1021 acatcgcggg actgctcgtg aagcgggggg ccatgcactg gggcgacgag ccggtctcgg
     1081 cgctggacca cgcgctgcag tgcgcggcgc gcgcccgggc cgcgcgcgcg gccgacgagg
     1141 tcgtcctggc cgcgctgctg cacgacgtcg gccacctgat cagcgacagc gaggagaccg
     1201 agttcagcca ccacggcctg tgggccgcgc gcttcctgcg tccgttcgtg cccgcgcgag
     1261 tggcctggct cgtcgaatac cacgtgctgg ccaagcggta cctctgctcg gtcgatcggg
     1321 tatacgcgga gagcctctcg ctcgggtcga cacgctcgtg gatccgccag ggcggggtgc
     1381 tcgacctcga cgcgcggcga gagatggagc gccagccgtg gctcggcgag gccctggcct
     1441 tgaggcgatg ggacgaggag gccaagcacc ccggtgtggt cgtgcccgcg ctgggcgact
     1501 accgcgagct gctggaggcg tgcttcggcc gccagctctg ggaggcggcg gcggtgggcg
     1561 gtcatgatct tgcagcgagc ttgtaatcca attgtcacgg agcgccgggt agtatcccgg
     1621 ccaaaggggg agcacagcgc gtgaatcgac tgatcgtgtg ggcgttggcg gtcgggctga
     1681 ccgtggtcgg cccgtcggtg gcgggatcgc aggatgggct gcggctgacc ggatcggggg
     1741 cgacgttccc gttcccgctg tactcgacgt ggttcaaggc gttcagctcc aagcagaagg
     1801 tcgtgagcgt cgactaccag ggcaagggga gcggcgccgg catccaggac ctgctaaacc
     1861 acacggtcga cttcgcggcg agcgacgcgg ccatgaccga cgaggacatg gccaaggtgg
     1921 cggggggcgt ccagctcttg ccgatgaccg cgggcgagat cgtgctggcc tacaacttgc
     1981 cgggcaaccc caaggcgctg aagctgcccc gcgacgtgta cgcgggcatc ttcctcggca
     2041 aggtcaccaa gtggagcgat ccccagctca aggccgcgaa cccgggcgtc acccttcccg
     2101 atcaggacat cacggtggt
//
LOCUS       DATMCV010000066         3497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_603330, whole genome shotgun sequence.
ACCESSION   DATMCV010000066 DATMCV010000000
VERSION     DATMCV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603330"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VNU03_01640"
     CDS             <1..984
                     /locus_tag="VNU03_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWP73178.1"
                     /translation="TAFRGTIETGEGPTIAILCEYDALPGIGHACGHNVIATSGVGAG
                     AALAAVRAQLPKGRIQVIGTPAEEGGGGKVKLIKGGVFRDVDCAMMIHGFDRTLLHQD
                     LLGIVRGTFEFTGRASHASADPWEGVNALDAVIQTYNAISMLRQQVRPDCRIHGIITN
                     GGAAANIIPEYASAIFYVRAPRIDTMWELFKRVTAAAEGAAKATGCTLKITQHDSVYE
                     PMKSSRVMLDLFAANMKTAGLTEGAPIPDRLGSSDIGNVSQVIPAIQPMVAIAPTGMA
                     IHTRDFADAAVKPLARAGMVAAAKTMALTTLDLLAEPARVKAAKDEFAKSK"
     gene            989..2182
                     /locus_tag="VNU03_01645"
     CDS             989..2182
                     /locus_tag="VNU03_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWP73179.1"
                     /translation="MTAPAELEQAKARVAETVDRLADELERISHQIHANPELCFEEHK
                     AAGWLADFLDRQGAAVERGVGGLPTAFRATIAGAGPGPTIAIMAEYDALPNIGHACGH
                     NVIATAGTGAGAAIAAALRTLPFPGRIQVIGTPAEEGGAGKVRLMEAGVFQGVDAAMM
                     IHGRCGTQVWRPSLGIIKVKCEFFGRASHASSWPWRGVNALNAMIQLFVSLDAMRQQL
                     RPDARVHGIITKGGDQANIIPEHTASEFYLRAPTKDYCRELLRRFEGCAAGAATATGC
                     TVKVTADATIHDPLKANFVMAELFGKNLERIDFPVDPDDGEAGYGSTDCGNVSQAIPT
                     IHPYIRISPDGIPGHSREFAEWAKSPLARTGMVAAAKALAMTALDLVARPDELEKAKR
                     EFARA"
     gene            complement(2201..2656)
                     /locus_tag="VNU03_01650"
     CDS             complement(2201..2656)
                     /locus_tag="VNU03_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73180.1"
                     /translation="MRLTRRLGVAVSLLVLAATWPGAALAADAPGSPNLHLSISETPG
                     ETTVRVPLASESYRLYGLLSPYLSLGSATTLGVPWSASLVPGLQRDTDGLDDVRLGAG
                     MALPLSDRAQLFGEYRFLRGRVDSGAGRSLLQREPDSSDFRAGFSIRLD"
     gene            complement(2679..3242)
                     /locus_tag="VNU03_01655"
     CDS             complement(2679..3242)
                     /locus_tag="VNU03_01655"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP73181.1"
                     /translation="MTMPPEQAKAKTYLLEKGTQAPVAQIRERVAEAFAAMDTLLEGV
                     TEAQARRAPGGGEWSVHEILDHLVETHPRALEEMRALLANRHSPVSPIPAGLQSAAPM
                     ARRWDDLRDGLRRIHTEVLDVLAAAPDRPTEARAPVIMVINTRDADGRLTPLHWNEAC
                     DWKACAVIFRLHELDHLSQTRKALRAT"
     gene            complement(3239..>3497)
                     /locus_tag="VNU03_01660"
     CDS             complement(3239..>3497)
                     /locus_tag="VNU03_01660"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWP73182.1"
                     /translation="DRRLAAVNRQAYVRARRLIGAALARGRAEGGVRRVDLEQAAAVV
                     LGLLDGLSLQLTFDRGLMTLPRAARLAETVLVRYLSPGDPT"
BASE COUNT          532 a         1282 c         1244 g          439 t
ORIGIN      
        1 accgcgttcc gcggcaccat cgagaccggg gaaggcccga ccatcgcgat cctgtgcgag
       61 tacgacgccc tccccggcat cgggcacgcg tgtgggcaca acgtgatcgc gacctccggt
      121 gtcggcgcgg gcgcggccct cgccgcggtc cgggcccagc tcccgaaggg ccgcatccag
      181 gtcatcggca ccccggcgga ggagggcggc ggcgggaagg tgaagctgat caagggcggt
      241 gtcttccgcg acgtggactg cgccatgatg atccacgggt tcgaccgcac cctgctccac
      301 caggacctgc tcggcatcgt gcgcgggacc ttcgagttca ccggccgagc ctcccacgcc
      361 tccgccgacc cgtgggaggg cgtgaacgcc ctcgacgcgg tcatccagac gtacaacgcg
      421 atcagcatgc tgcgccagca ggtgcgcccc gactgccgga tccacggcat catcaccaac
      481 ggaggggcgg cggccaacat catcccggaa tacgcgtccg cgatcttcta cgtgcgggcc
      541 ccgcgcatcg acacgatgtg ggagctcttc aagcgggtga ccgcggccgc ggagggcgcc
      601 gcgaaagcga ccgggtgcac cctcaagatc acccagcacg acagcgtcta cgagccgatg
      661 aagtcgagcc gcgtgatgct cgacctcttc gcggccaaca tgaagacggc ggggctcacc
      721 gagggcgccc cgatcccgga ccggctcggc tcctccgaca tcggcaacgt gagccaggtg
      781 atcccggcca tccagccgat ggtggccatc gcccccaccg gcatggccat ccacacccgc
      841 gacttcgcgg acgcggcggt gaagccgctc gcgcgcgcgg gcatggtcgc ggcggccaag
      901 accatggccc tcaccaccct cgacctgctc gccgagccgg cccgcgtgaa ggcggcgaag
      961 gacgagttcg ccaagagcaa gtgacgtcat gaccgcgccc gccgagctcg agcaggccaa
     1021 ggctcgcgtc gccgagacgg tggaccggct cgccgacgag ctcgagcgca tcagtcacca
     1081 gatccacgcc aatcccgagc tgtgcttcga ggagcacaag gcggcgggct ggctcgccga
     1141 cttcctcgac cggcagggcg ccgcggtgga gcgcggggtg ggcgggctgc ccaccgcgtt
     1201 ccgggccacc atcgcgggcg cgggccccgg gcccaccatc gcgatcatgg ccgagtacga
     1261 cgcgctgccg aacatcgggc acgcctgcgg gcacaacgtg atcgcgaccg cgggcaccgg
     1321 cgcgggggcg gcgatcgcgg cggcgctgcg gaccctgccc ttcccgggcc gcatccaggt
     1381 gatcggcacg cccgccgagg agggcggggc cggcaaggtc cggctgatgg aggcgggcgt
     1441 cttccagggg gtggacgcgg ccatgatgat ccacggccgc tgcggcaccc aggtctggcg
     1501 gccgagcctc ggcatcatca aggtcaagtg cgagttcttc ggccgcgcct cgcacgcgtc
     1561 gtcctggccg tggcgcgggg tcaacgcgct caacgcgatg atccagctct tcgtctcgct
     1621 cgacgccatg cggcagcagc tccgcccgga cgcccgcgtg cacggcatca tcaccaaggg
     1681 cggcgatcag gccaacatca tccccgagca caccgcctcc gagttctacc tgcgcgcgcc
     1741 caccaaggac tactgccgcg agctgctgcg gcggttcgag ggctgtgcgg cgggcgcggc
     1801 gaccgccacc gggtgcaccg tgaaggtgac cgcggacgcg accatccacg accccctgaa
     1861 ggccaacttc gtgatggcgg agctcttcgg caagaacctc gagcggatcg acttcccggt
     1921 ggatcccgac gacggagagg cgggctacgg gtcgaccgat tgcggcaacg tgagccaggc
     1981 gatcccgacg atccatccct acatccgcat ctcgccggac gggatccccg gacattcgcg
     2041 cgagttcgcg gagtgggcca agtccccgct cgcgcgcacc ggcatggtgg cggcggccaa
     2101 ggccctcgcc atgaccgcgc tcgacctggt ggcgcggccg gacgagctcg agaaggcgaa
     2161 gcgggagttc gcgcgggcct agccggggcc cggcccgcgc tcagtcgagg cggatcgaga
     2221 agccggcgcg gaagtccgag gaatcgggct cccgctggag caggctgcgg cccgcgccgc
     2281 tgtcgacgcg gccgcgcagg aagcgatact ccccaaagag ctgcgcccgg tccgagagcg
     2341 gcagcgccat ccccgccccg aggcgcacgt cgtcgagccc atccgtgtcc cgctggagcc
     2401 cggggacgag gctcgcgctc cacggcaccc cgagcgtcgt ggcggagccc agcgagaggt
     2461 atgggctcag caggccgtac agccggtagc tctccgaggc caggggcacg cgcaccgtcg
     2521 tctcccccgg ggtctcgctg atcgagaggt gcaggttggg ggacccgggc gcatcggcgg
     2581 cgagggccgc tcccggccac gtggccgcga ggacgagcag tgagaccgcc accccgagcc
     2641 gccgcgtgag ccgcatgatt gtaaccttac acgacgcgtc aagtcgcccg gagcgccttg
     2701 cgagtctggc tcaggtggtc cagctcgtgg agccggaaga tcaccgcaca ggccttccag
     2761 tcacacgcct cgttccagtg gagcggcgtc aggcgaccgt cggcgtcccg ggtgttgatc
     2821 accatgatga cgggcgcgcg agcctcggtc ggccgatcgg gcgcggcggc caggacgtcg
     2881 agcacctcgg tgtggatgcg gcgcagccca tcgcgcaggt cgtcccaccg gcgggccatc
     2941 ggcgcggccg attgcaggcc ggccggaatc gggctcaccg gcgaatggcg gttcgcgagc
     3001 agcgcgcgca tctcctcgag ggcgcgcgga tgcgtctcca ccaggtggtc caggatctcg
     3061 tgcacgctcc actcgccgcc gcccggtgcg cgccgcgcct gggcctcggt gacgccctcc
     3121 agaagagtgt ccatcgcggc gaacgcctcg gccacgcgct cgcggatctg cgcgaccggg
     3181 gcctgcgtgc ccttctccag gaggtacgtc ttcgccttcg cctgctcggg gggcatcgtc
     3241 atgtcgggtc tccgggtgag aggtagcgga ccagcaccgt ctcggcgagc cgcgccgcgc
     3301 gcggcagggt catcagcccg cgatcgaacg tgagctgcag cgagaggccg tcgaggaggc
     3361 ccaggaccac cgcggcggcc tgctcgaggt cgacccggcg caccccaccc tcggcccgcc
     3421 cgcgcgccag cgccgcgccg atcaaccgcc gggcgcgcac gtaggcctgc cggttcaccg
     3481 cggccagtcg gcgatcg
//
LOCUS       DATMCV010000067         2988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_869316, whole genome shotgun sequence.
ACCESSION   DATMCV010000067 DATMCV010000000
VERSION     DATMCV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869316"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VNU03_01665"
     CDS             <1..484
                     /locus_tag="VNU03_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73183.1"
                     /translation="AARGVALVGKGHTPESDFSPQTVRHIKDHVPPGTLLAANQMQLW
                     AFTLDYGFLMIPWDQPLDNWPRDYGISPWTRLEALKVFVEKDVRYVVLFFGPTHGERV
                     LARYNPGQYVGSSLLTEPLPEVAGVTRLSDGVLVTLADREQLRELLAKEPPSGARPRQ
                     "
     gene            complement(620..1579)
                     /locus_tag="VNU03_01670"
     CDS             complement(620..1579)
                     /locus_tag="VNU03_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73184.1"
                     /translation="MLPRQGVTVEEALLALLAGVAIVLLFLGLADALEGDARARLRAR
                     RRALARRSPVPRAAPEPPRADRPALARRPAAGRPRAVEAPRVAPAPVPEPATEPTEGD
                     APEWRDRAAALMRGREWGEARREVESALAAGDARPQSVSFLLEVCSTATARELWRLRR
                     ALRRGVGDETPLGGALETTRLLLDSEPAAELSPATRGRAGRRLWRGQARLGLRRWRAG
                     EFDAAVEALFLALAVPGLDDRRRALARDLLARTLEDMAGQRLELIPQLLGDGDRPAAL
                     EQAQWLAAHVGRAREEGIAAEDLAVAAARARQLLEHIEHTPAR"
     gene            1733..2887
                     /locus_tag="VNU03_01675"
     CDS             1733..2887
                     /locus_tag="VNU03_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWP73185.1"
                     /translation="MRRVAVIGVGVTKFGKHDRTSAELFAQAGADAILDAGIAPSAIQ
                     ALYFGNVTGGETERQLHMSPLAATLLGLPNVPTTRFENACATSHVAFRHAVMEIASGV
                     SDVILVGGAERVLNVPTESATEFFAYCSDSSLEQPAGLTFPGVFALIARAHMDKYGTT
                     EEQMAHVAVKNHHHGTLNPKAQFQKEISLETVLKSAYVADPLKLYDCCPFTDGGAALV
                     LASEEVARRHRRPIWVLGTHAASDTMFVQEKRDLSRVTATERAAQGAYRQAGKTPADV
                     DVVELHDCFTIAEIVATEGLGFFEPGTGGLAAEKGWTSLGGKLPVNPSGGLKAKGHPI
                     GATGAAQIAEIVTQLRGEAGKRQVEGATTGLTHTLGGNTATVLVSLFGRD"
BASE COUNT          436 a         1110 c         1047 g          395 t
ORIGIN      
        1 cgctgctcgc ggcgtggctc tggtggggaa agggcacacg ccagagtcgg acttctcgcc
       61 gcagacggta cgccacatca aggatcatgt gccgcccggc acgctgctgg ccgcgaacca
      121 gatgcagctc tgggcgttca cgctcgacta cggctttctc atgatcccct gggatcagcc
      181 cctggacaat tggccgcgag actacggcat cagcccgtgg acgcggctcg aggcgctgaa
      241 ggtgttcgtg gagaaggatg tgaggtacgt cgtactcttt tttggaccca cgcacggcga
      301 gcgggtcctg gcgaggtaca acccggggca gtacgtgggt tcatctcttc tgacggagcc
      361 tcttcccgag gtcgcaggcg tgacgcggct gtcggatggt gtcctcgtga ccctcgccga
      421 ccgagagcaa ttgcgagagc ttctcgcgaa ggagccgcca tcgggtgcga ggcctcggca
      481 atgagtcggc ggacatccat cgctccgcgt ccggccgcac cctcctcgct ggagaggtcg
      541 agctagtcga tcgaggagcc ctcgggagtg catcggaccg gcgcggggat ctggcgcggg
      601 gtcatgcggc caagatcgat tatcgcgccg gcgtgtgctc gatgtgctcc aggagctgac
      661 gggcccgcgc ggccgcgacc gccaggtcct ccgccgcgat gccctcctcg cgcgcccgcc
      721 cgacgtgggc cgcgagccac tgggcctgct cgagcgcggc gggccggtcc ccgtctccca
      781 gcagctgcgg gatcagctcg aggcgctggc ccgccatgtc ctcgagcgtg cgcgccagca
      841 ggtcccgcgc gagcgcgcgc cggcgatcgt cgagaccggg caccgccagg gccaggaaca
      901 gcgcctcgac cgcggcgtcg aactcgcccg cgcgccagcg tcgcagtccc agacgagcct
      961 ggccgcgcca gagccgccgg cccgcccggc cccgcgtcgc cggggagagc tcggccgccg
     1021 gctccgaatc gaggagcagg cgcgtcgtct cgagggcgcc gccgagcggc gtctcgtcgc
     1081 ccaccccgcg ccgcagcgcc cggcgcagtc gccagagctc gcgcgcggtg gcggtggagc
     1141 agacttcgag caggaagcta acgctctgcg ggcgggcgtc acccgcggcg agcgccgact
     1201 ccacctcccg ccgggcctcg ccccactcgc gaccgcgcat cagagcagcg gcgcgatccc
     1261 gccattcggg cgcgtccccc tcggtgggct ccgtggcggg ctccgggaca ggtgccgggg
     1321 cgacccgcgg ggcttccacc gctctgggcc gcccggcggc cggccgccgc gcgagggccg
     1381 ggcggtccgc gcgaggtggc tcgggcgcag cccgcgggac cggcgaccgg cgagccaatg
     1441 cgcgtcggcg cgcgcggagc cgggcgcgcg cgtcgccctc gagcgcgtcc gccagcccca
     1501 ggaacagcag cacgatcgcc acgccggcga gcagcgcgag cagggcctcc tcgacggtca
     1561 cgccctgcct cgggagcatc cgaccggcgt catgcggccc accgtaccat ggccccccgg
     1621 cattccacga cgatttgccg ggtcgggagc cacctttgag ttcgcgtggc gcggcgcggt
     1681 atgatgccag cagcgaccga ttcgcaatct tcgccggacc agaggagacc gcatgcgccg
     1741 cgtggccgtc atcggagtgg gtgtcaccaa gtttggcaag cacgatcgca ccagcgccga
     1801 gctgttcgcg caggccggcg ccgacgccat cctcgacgcg ggcatcgcgc cgagcgcgat
     1861 ccaggccctc tacttcggca acgtcaccgg cggtgagacc gagcggcagc tgcacatgtc
     1921 cccgctggcc gccacgctgc tcggcctgcc caacgttccc accacccgct tcgagaacgc
     1981 ctgcgcgacc agccacgtcg cgttccggca cgcggtcatg gagatcgcga gcggggtgtc
     2041 ggacgtgatc ctggtcggcg gcgcggagcg ggtcctcaac gtgccgaccg agtccgcgac
     2101 ggagttcttc gcctactgct cggactcctc cctcgagcag cctgcgggac tcaccttccc
     2161 cggcgtcttc gccctgatcg cgcgcgccca catggacaag tacggcacca ccgaggagca
     2221 gatggcccac gtggcggtca agaaccacca ccacggcacc ctcaatccca aggcccagtt
     2281 ccagaaggag atctcgctcg agacggtgct caagagcgcc tacgtcgccg atcccctcaa
     2341 gctctacgac tgctgcccct tcaccgacgg gggcgcggcg ctcgtgctgg cttccgagga
     2401 ggtcgcgcgc cggcaccggc ggccgatctg ggtcctgggc acgcacgcgg caagcgacac
     2461 catgttcgtg caggagaagc gcgacctctc gcgcgtcacc gccaccgagc gggccgcgca
     2521 gggggcctac cggcaggcgg gcaagacccc cgccgacgtc gacgtggtgg agttgcacga
     2581 ctgcttcacc atcgccgaga tcgtggccac cgagggcctc ggcttcttcg agcccggcac
     2641 cggcggcctc gcggccgaga agggctggac cagcctcgga ggcaagctcc cggtcaaccc
     2701 gtccggcggc ctcaaggcca aggggcatcc gatcggcgca accggggccg cccagatcgc
     2761 cgagatcgtc acccagctcc gcggcgaggc cggcaagcgg caggtcgagg gggcgacgac
     2821 cgggctcacc cacacgctgg gcgggaacac cgcgaccgtc ctggtgagcc tgttcggccg
     2881 tgactgagca cgtgatccac ctgaaagact tcttcgagga ggcccgcgcc gggcacctga
     2941 ccgccatccg ctgcggacgg tgcggggccc tggcgattcc tcccaagg
//
LOCUS       DATMCV010000068         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_869323, whole genome shotgun sequence.
ACCESSION   DATMCV010000068 DATMCV010000000
VERSION     DATMCV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869323"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..885
                     /locus_tag="VNU03_01680"
     CDS             34..885
                     /locus_tag="VNU03_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP73186.1"
                     /translation="MSEVRVATLEGRFTADIAGPADGALVLLLHGYPQTRHTWRDQVP
                     ALGAAGYRAVAPDQRGYSPGVRPDPAQGLAAYGIDRLVQDVLDLADASGAAGQPFHLV
                     GHDWGGHVAWVVAHRHPGRLRSLTVLSRPHPAAFRRAFKENADDQQHRSRHHRIFHDP
                     TTGPRLLEDGARRLRAGLADHGVGSAAVAEYVSVLGEPAAQEAALAWYRAAGTLTAVE
                     VGPVTVPTLYIWGDGDATVGPSAARFTADFVTGPFRFEIVPGVGHFVTDQAPAAVTRL
                     LLSHLGG"
     gene            986..1909
                     /gene="ilvE"
                     /locus_tag="VNU03_01685"
     CDS             986..1909
                     /gene="ilvE"
                     /locus_tag="VNU03_01685"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR01122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HWP73187.1"
                     /translation="MAKELKIWMNGRLVSQAEAVLPVNSAAVFYATNVFEGLRAYWNE
                     ADGELYCFRLAEHFARLRESMKMMRFTVSYSDMDIYEAVREVLRGNEIREDVHMHLVA
                     YVSAPGLDSTGPTGLYINPRRRGRISDGDGLRCCITSWQRTHDNAIPIRLKCGANYQN
                     GRLATLQAKADGYDAPIFLNQQGTVAEGSGATFFMVRRGKLITPPITSDILESITRTT
                     LIDAVAPALGMEVVERDIARTELYVADEAFFCGSGYEITPILSVDRFPLGDGGVGAIT
                     QRLLTAYMNVVRGADRRFPEWRMPVYKPVTV"
BASE COUNT          291 a          768 c          685 g          302 t
ORIGIN      
        1 tgagccgcag ccggaggcga ggagaacgca ttcatgagtg aagtcagggt ggcgacgctt
       61 gaaggccgat tcaccgccga catcgccggc cccgccgacg gcgcgctcgt cctgctcctg
      121 cacggctacc cgcagacgcg ccacacctgg cgggaccagg tccccgcgct cggcgcggcc
      181 ggctaccgcg cggtggcccc ggaccagcgc ggctactccc cgggggtgcg gccggacccg
      241 gcccaggggt tggccgcgta cggcatcgac cggctggtgc aggacgtgct cgacctggcc
      301 gacgcgagcg gcgcagccgg ccagcccttc cacctggtcg gccacgactg gggcggccac
      361 gtggcgtggg tcgtcgcgca ccgccatccc ggacggctcc gctcgctcac cgtcctgtcg
      421 cggcctcatc cggccgcgtt ccgccgggcc ttcaaggaga acgccgacga tcagcagcac
      481 cgctcccgcc accatcgcat tttccacgat cccaccaccg gtccccgcct gctcgaggac
      541 ggcgcccggc gcctgcgcgc cggactggcc gaccacgggg tgggctccgc cgcggtcgcg
      601 gagtacgtct ccgtgctggg cgagccggcc gcgcaggagg cggccctggc ctggtaccgc
      661 gcggccggaa ccctgaccgc ggtcgaggtc ggcccggtca cggtgccgac gctctacatc
      721 tggggagacg gcgacgccac cgtcggaccc tcggcggccc ggttcaccgc cgatttcgtc
      781 accgggccct tccgcttcga gatcgtgccg ggggtgggcc acttcgtgac cgaccaggcc
      841 cccgcggcgg tcacccggct gctcctctcc catctgggcg gctaagccca tttcggcggt
      901 gaagcccatt tcggcggtca ggaatgatat cctctctgcg gtcgcgcgcc gcagcgcagg
      961 ggcgccgaca acggaggatc ccatcgtggc gaaagagctg aagatctgga tgaacggccg
     1021 gctggtctcc caggccgagg ccgtcctgcc cgtgaacagc gcggccgtgt tctacgcgac
     1081 caacgtgttc gagggcctgc gggcgtactg gaacgaggcg gacggggagc tctactgctt
     1141 ccggctcgcc gagcacttcg cgcgcctccg cgagtccatg aagatgatgc gcttcacggt
     1201 ctcctacagc gacatggaca tctacgaggc ggtgcgcgag gtcctgcggg gcaacgagat
     1261 ccgcgaggac gtgcacatgc acctcgtggc ctacgtgtcg gcgcccggcc tcgactcgac
     1321 cgggccgacc gggctctaca tcaatccccg ccgccgcggc cgcatctcgg acggcgacgg
     1381 cctgcgctgc tgcatcacct cctggcagcg cacccacgac aacgcgatcc cgattcgcct
     1441 gaagtgcggg gccaactacc agaacgggcg gctcgccacg ctgcaggcca aggcggacgg
     1501 ctacgacgcg ccgatcttcc tgaaccagca gggcacggtg gccgagggct cgggggccac
     1561 cttcttcatg gtccgccggg gcaagctcat caccccgccg atcacgagcg acatcctcga
     1621 gtcgatcacc cgcaccaccc tgatcgacgc ggtggcgccc gcgctcggca tggaggtggt
     1681 ggagcgcgac atcgcccgca ccgagcttta cgtggccgac gaggccttct tctgcggcag
     1741 cggctacgag atcaccccga tcctctcggt cgatcgcttc ccgctgggcg acggcggggt
     1801 gggcgcgatc acccagcgcc tgctcaccgc gtacatgaac gtggtgcgtg gcgccgaccg
     1861 ccgcttcccc gagtggcgca tgccggtgta caagcccgtc accgtctgat tcgatcattc
     1921 atcctcctcc ccaccctctc cccgcccaag gggagagggt ggagcgaggg agcctccgtc
     1981 atgaccggcg ccatccgtgt ccgtgggctt tttctgatcc tggcgatcac cctcgcggcc
     2041 gctccc
//
LOCUS       DATMCV010000069         2169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_404038, whole genome shotgun sequence.
ACCESSION   DATMCV010000069 DATMCV010000000
VERSION     DATMCV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404038"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VNU03_01690"
     CDS             complement(<1..365)
                     /locus_tag="VNU03_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP73188.1"
                     /translation="MQAVHADDREWETLRWPGQESKMLFHPSPERPTAPNAGLVRYEP
                     GSHHPFHKHDFAQVWYILEGEFQIGETFHGPGSMLFYPDPHFEAPLSTHTGGLMLFVQ
                     YQGPTTGGRPIYDGRFNMT"
     gene            complement(413..871)
                     /locus_tag="VNU03_01695"
     CDS             complement(413..871)
                     /locus_tag="VNU03_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73189.1"
                     /translation="MDSRQPSLLEAPASTRAARVQPSRLLPRSPRGAVEIVRHANGPV
                     EVKVSGLIDSAERRIRQWQGVAAMLFIVMVVGAALAVVVVALRWDYERGRAVAVLQAD
                     LEIARARERCWEALVRYTPRGPEDVVTPARRDGWIGRCLATELGRVNARK"
     gene            complement(892..2022)
                     /locus_tag="VNU03_01700"
     CDS             complement(892..2022)
                     /locus_tag="VNU03_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP73190.1"
                     /translation="MMTHRTIGTLSLLMTLVVCVLAPSPAWAHRPTDPPHQLYQMGDL
                     TLESGQVIRDFAISYVTHGTLNAKKSNAILMVTALSGNHHRLDFLIGPGKALDPGKYF
                     IVCTDAISNGLTTSPSNSKAQPRMQFPRFAMRDMVQSQHRLLTEKLGITRVAAVIGPS
                     MGGMQALQWGVSHPDFMDSLVAIVPLARTPAWSTVVVDASRKAIMLDPAWNGGNYTAP
                     PVEGLRLFRDVVGFMAARTPEMYRHQFDKPLDVLPWLKQQEDAAIPVFDANDYIYQSW
                     AYEAHDVSTTPGMGGDYAKALRAIKAKTLIMVGVKDLLNPDWEPLEAARYIRDVRTLT
                     INPSSVTGHAAAGGFFPADVDFMNVEIGQFLDLVTQRGEKLR"
     gene            complement(2038..>2169)
                     /locus_tag="VNU03_01705"
     CDS             complement(2038..>2169)
                     /locus_tag="VNU03_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73191.1"
                     /translation="KNVIREAFWVSLFPGLALTIAGLSLNLLGDGLRDVLDPRLRVR"
BASE COUNT          320 a          730 c          766 g          353 t
ORIGIN      
        1 tccgtcatgt tgaagcgccc gtcgtagatc ggccggccgc ccgtcgtcgg gccctggtac
       61 tgcacgaaca gcatcaggcc cccggtgtgg gtggagagcg gcgcctcgaa gtgcggatcc
      121 gggtagaaga gcatcgagcc cggcccgtgg aacgtctcac cgatctggaa ctcgccctcg
      181 aggatgtacc agacctgggc gaagtcgtgc ttgtggaagg ggtggtgcga gcccggctcg
      241 taccgcacca gccccgcgtt gggcgcggtg gggcgctcgg ggctcgggtg gaagagcatc
      301 ttgctctcct gccccggcca gcgcagcgtc tcccactccc ggtcgtcggc gtgcaccgct
      361 tgcattggct ggtgaattgg gggctgatga tcctgcgaca tgaggtctcg gctcactttc
      421 tggcgttcac gcgcccgagc tcggtggcga ggcagcgccc gatccagccg tcgcggcggg
      481 cgggcgtgac gacgtcctcg ggcccgcgcg gcgtgtagcg caccagcgcc tcccagcagc
      541 gctcgcgcgc gcgggcgatc tcgagatcgg cctggagcac cgcgaccgcg cggccccgct
      601 cgtagtccca gcgcagcgcc accaccacca ccgcgagcgc ggcgcccacc accatgacga
      661 tgaagagcat cgccgccacg ccctgccact ggcggatccg ccgctccgcg ctgtcgatca
      721 gtccggagac cttcacctcc accgggccgt tggcgtgccg cacgatctcc accgcgccgc
      781 ggggcgagcg cggcagcagc cggctcggct ggacccgcgc cgcgcgcgtg gacgcgggcg
      841 cttccagcag ggagggctgt ctcgagtcca tcgcctccat catccgacct cctaccgcag
      901 cttctcgccg cgttgagtca cgaggtcaag gaactggccg atctcgacgt tcatgaaatc
      961 gacgtcggcc ggaaagaatc cgcccgcggc ggcgtggccg gtgacggacg acggattgat
     1021 cgtgagggtg cgcacgtcgc ggatgtagcg cgccgcctcg aggggctccc agtcgggatt
     1081 cagcaggtcc ttcaccccca ccatgatgag ggtcttggcc ttgatggccc gcagcgcctt
     1141 cgcgtagtcc ccgcccatgc cgggggtggt cgagacgtcg tgggcctcgt aggcccacga
     1201 ctggtagatg tagtcgttgg cgtcgaagac cgggatcgcc gcgtcctcct gctgcttgag
     1261 ccagggcagc acgtcgagcg gcttgtcgaa ctggtggcgg tacatctccg gcgttcgcgc
     1321 ggccatgaag ccgaccacgt cgcggaagag gcgcagcccc tccaccggcg gcgcggtgta
     1381 gttgccgccg ttccacgccg gatcgagcat gatcgccttg cgggaggcgt cgaccaccac
     1441 cgtgctccag gccggggtgc gggccagcgg cacgatggcc accaggctgt ccatgaagtc
     1501 gggatggctc acgccccatt ggagcgcctg catcccgccc atcgaggggc cgatgaccgc
     1561 ggcgacccgg gtgatcccga gcttctcggt cagcaaccgg tgctgcgact ggaccatgtc
     1621 ccgcatcgcg aagcgcggga actgcatccg cggctgcgcc ttgctgttgc tgggcgaggt
     1681 ggtgagcccg ttcgagatcg cgtcggtgca gacgatgaag tacttgccgg ggtcgagcgc
     1741 cttgccgggt ccgatcagga aatcgaggcg atggtggttg ccgctgagcg cggtgaccat
     1801 caggatcgcg ttggatttct tggcgttgag ggtcccgtgg gtcacgtagg agatggcgaa
     1861 gtcccggatg acctggccgc tctcgagcgt gaggtcgccc atctggtaga gctgatgcgg
     1921 cgggtcggtc ggccggtggg cccaggccgg ggagggagcg agcacgcaca cgaccagcgt
     1981 catcagcagg ctcagcgtcc cgatcgtgcg gtgtgtcatc attcggtgcc cccctttcta
     2041 gcgcactcgg agccgcgggt ccagcacgtc tcgcaatcca tcgcccagga ggttgaggct
     2101 cagcccggcg atggtcaagg ccaggccggg aaagagactc acccagaacg cctcgcggat
     2161 gacgttctt
//
LOCUS       DATMCV010000070         4794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_869415, whole genome shotgun sequence.
ACCESSION   DATMCV010000070 DATMCV010000000
VERSION     DATMCV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869415"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VNU03_01710"
     CDS             complement(<1..268)
                     /locus_tag="VNU03_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWP73192.1"
                     /translation="MTADELCYAGIRELGGLFRRGALSPVDYATDLLGRVERLDKSLN
                     SYVTVTAERALADARAAEAAIRRGDAGPLAGIPIAYKDLYATKGI"
     gene            complement(265..471)
                     /locus_tag="VNU03_01715"
     CDS             complement(265..471)
                     /locus_tag="VNU03_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73193.1"
                     /translation="MPELPPEVTLDEVKERARLAGLPIPASRLPMVRAFLVNALRPIR
                     ALDTRTVRTVEPAVTFDAKGGAPR"
     gene            604..2136
                     /locus_tag="VNU03_01720"
     CDS             604..2136
                     /locus_tag="VNU03_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73194.1"
                     /translation="MRDTSAASHPVGRRQFLTLSAAGLAAAGLPRAARAQGKRSGELV
                     AGLSERMLTLDPANHYSISSTSVHRHMFDPLVDVTNDSKFVPALAESWRAVNNTTWRF
                     TLRKGVTFHDGTPFNADSVLYTLRRVKDNSKLIKSFVYQDIESVEKDGDFAVVITSKR
                     PFGSLPAHLTMLGMLPPGAGKNEDAFFQKPIGTGPFRFVSWTHGDHIVLSANPSYWKP
                     AQPRVEKLTFRFIPELSTRASALRAGEIHVIDRVTPDLVETLKGSRGVKVFDVPAVEA
                     QRWVFQLAKEPVKDQRLRQAISLAIDRNAIIKDLLLGYARPVDSPVPPGLIGHTSLPA
                     KPYDPEKARQILKQAGYSNVTLDFVLMKDLYPKQLEISQAVAAMLGEVGIKVNIKNLE
                     IATAREQRSAGTYDMFFSGWAHMPHDPDWYFGQWFTKAGSEKLTRYNNPRVEQLIAEG
                     RVPDPKVRQAKYEELERILWDEDAEIWPYYSVAIYGTSDRLRNFEPRRDYYVLLSDVA
                     IA"
     gene            2170..3090
                     /locus_tag="VNU03_01725"
     CDS             2170..3090
                     /locus_tag="VNU03_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73195.1"
                     /translation="MKAYVLRRLWQSALTLVGVSVLVFVILRVVPGDPAKMLLPDGAP
                     QSAVDELNRQLGLHEPLYVQYGLFLQSVFRGDFGQSFQYRAPALRVVVERLAATVQLA
                     LAALLITVGVGVSLGILAAVRRGTGYDYASTVLAVLGQSLPNFWLGIMLILLFGVALR
                     WLPTSGFESWRHLILPAVTLAAFPMALVARLTRSSMLEILGRDFIRTGRAKGLAERAV
                     ILRHALRNAAVPLLTVLGLQIGTLLGGAVITESVFAWPGMGKLVVDAIFFRDFPVVQT
                     VLILSATIFVVINLLVDLLYTVIDPRIRYS"
     gene            3087..3944
                     /locus_tag="VNU03_01730"
     CDS             3087..3944
                     /locus_tag="VNU03_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73196.1"
                     /translation="MSAPVAAEAARHSGRLSPPALVGGVLLALVVLASVLAPLLASSD
                     PVKPSFSRRLAPPWGLGGTPAHVLGTDNLGRDILARVLHGGRISLALATVSVLIAALV
                     GILVGLVAGSAGERLDGLLMRAADVQLAFPVIMLAIAIVAVVGTSPGALVGVLALSGW
                     VLYARTVRASVLTIRRLEYVEAAYTLGASDLRIIARHILPNTIAPILVLGSSQFATMV
                     LLESGLSFLGMGIQAPRPSWGLMLAEGRDYLSNAWWLATIPGVAISLVVLGANLLGDG
                     LRDLLDPRG"
     gene            complement(4026..>4794)
                     /locus_tag="VNU03_01735"
     CDS             complement(4026..>4794)
                     /locus_tag="VNU03_01735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP73197.1"
                     /translation="AFPGQGAALEQLSLMAFVAGKSRRLRLISSVMILPHRNPVVTAK
                     MLATIDVLSRGRVTVGVGVGWLREEFQALGAPDFDRRGAVSDEYLRIFKTLWTQDPAT
                     FHGEFYRFDRIHCLPHPAQKPHPPIWIGGHSKAALRRVARLGDGWHPVGANPAVPLRP
                     AELRAELDDLRRLTEAERRDFSTLTISYKAPIYDPAQGVDGGAERRPFSGSQQAIADD
                     IGAFAGLGVSELIFDFRAENLSESLERMERFAPLIRS"
BASE COUNT          668 a         1707 c         1674 g          745 t
ORIGIN      
        1 ggatgccctt ggtcgcgtag aggtccttgt aggcgatcgg gatgcccgcg agggggcccg
       61 cgtcgccgcg ccggatggcg gcctccgccg cgcgggcgtc cgccagcgcg cgctccgcgg
      121 tgaccgtcac gtaggagttg aggctcttgt cgaggcgctc gacgcggccg agcagatcgg
      181 tcgcgtagtc gaccggcgac agcgcgcccc ggcggaagag gccgccgagc tcccggatgc
      241 ccgcgtagca gagctcgtcg gcggtcatcg cggcgctccg ccctttgcgt cgaacgtcac
      301 cgcgggctcc accgtccgca cggtgcgggt gtcgagcgcg cggatcggcc gcagcgcatt
      361 cacgaggaac gcgcgcacca tgggcaggcg gctcgccggg atgggcaggc cggcgaggcg
      421 ggcgcgctcc ttcacttcgt ccagcgtgac ctcagggggc agctcgggca tggcgagtct
      481 ccttgtgagc gacgggccat agagtagacg ggtccgcgcg cgagagcaac tcatgggccc
      541 ggcgcggcct tgacagcgag ccgggcgatg gcatacaccc aggcccacag gaggagtgac
      601 cccatgcgcg acacctcggc agcatcgcat cccgtcggcc ggcgacagtt cctgaccctg
      661 agcgcggccg gcctggccgc cgccggcctt ccccgcgcgg cccgcgcgca gggcaagcgc
      721 agcggcgagc tggtggcggg cctctccgag cgcatgctca cgctcgatcc ggccaaccac
      781 tactcgatct cctccacctc ggtgcaccgg cacatgttcg atccgctggt ggacgtcacc
      841 aacgactcga agttcgtgcc cgccctcgcc gagtcgtggc gcgcggtcaa caacaccact
      901 tggcgcttca cgctgcggaa gggcgtcacc ttccacgacg gcaccccgtt caacgcggac
      961 agcgtgctct acaccctgcg gcgcgtgaag gacaactcca agctcatcaa gtccttcgtg
     1021 taccaggata tcgagtcggt ggagaaggac ggggatttcg cggtggtgat cacctcgaag
     1081 cggccgttcg gctcgctacc cgcgcatctc acgatgctcg gcatgctgcc gcccggcgcg
     1141 ggcaagaacg aggacgcgtt cttccagaag ccgatcggca ccggcccctt ccgcttcgtg
     1201 tcgtggacgc acggcgacca catcgtgctg tcggccaacc cgagctactg gaagccggcc
     1261 cagccgcggg tcgagaagct gaccttccgg ttcatccccg agctgtcgac ccgggcctcc
     1321 gccctgcgcg cgggggagat ccacgtgatc gaccgcgtca ctcccgacct ggtcgagacg
     1381 ctcaagggca gccggggcgt caaggtcttc gacgtgcccg cggtggaggc gcagcgctgg
     1441 gtcttccagc tcgccaagga gccggtcaaa gaccagcggc tgcgccaggc catctcgctc
     1501 gcgatcgacc gcaacgccat catcaaagat ctgctgctgg gctacgcgcg cccggtggac
     1561 agccccgtcc cgcccgggct gattggacac accagcctgc cggccaagcc ctacgatccc
     1621 gaaaaagcac gccagatcct caagcaggcc ggctattcca acgtgacgct cgacttcgtg
     1681 ctcatgaagg atctctatcc caagcagctc gagatcagcc aggcggtggc ggcgatgctg
     1741 ggcgaggtgg ggatcaaggt caacatcaag aacctcgaga tcgcgacggc gcgggagcag
     1801 cggagcgcgg ggacctacga catgttcttc tccggctggg cccacatgcc gcacgacccg
     1861 gactggtact tcggccagtg gttcaccaag gcggggtccg agaaactcac ccgctacaac
     1921 aacccgcgcg tcgagcagct catcgcggag gggcgcgtgc cggaccccaa ggtgcgccag
     1981 gcgaagtatg aggagctcga gcgcatcctg tgggacgaag acgccgagat ctggccgtac
     2041 tacagtgtcg ccatctacgg caccagcgac cggctgcgga acttcgagcc gcggcgcgac
     2101 tactacgtcc tgctgagcga cgtcgcgatc gcgtagcccg gtgacgcgcg ccccgcgggg
     2161 cgccggacga tgaaggccta cgtcctccgc cggctgtggc agtccgccct gaccctggtg
     2221 ggcgtgtcgg tgctggtgtt cgtcatcctg cgggtcgtcc ccggcgaccc cgccaagatg
     2281 ctcctgcccg acggcgcgcc ccagtccgcg gtcgacgagc tgaaccgcca gctcggcctg
     2341 cacgagcccc tctacgtcca gtacggcctg ttcctccaaa gcgtcttccg gggcgacttc
     2401 ggccagtcct tccagtaccg cgcccccgcg ctgcgggtgg tcgtcgagcg cctcgccgcc
     2461 accgtgcagc tcgcgctcgc ggccctgctg atcacggtgg gagtcggggt ctcgctcggc
     2521 atcttggccg cggtgcgccg cggcaccggc tacgactacg cgagcacggt gctggcggtg
     2581 ctcggccagt cgctgcccaa cttctggctc ggcatcatgc tgatcctcct cttcggggtc
     2641 gcgctgcggt ggctgcccac ctcggggttc gagagctggc gccacctcat cctgcccgcg
     2701 gtgaccctcg ccgcgttccc gatggctctg gtggcgcgcc tcacccgctc gagcatgctc
     2761 gagatcctcg ggcgcgactt catccgcacc gggcgcgcca agggcctggc cgagcgcgcg
     2821 gtcatcctcc ggcacgcgct ccgcaacgcg gcggtgccgc tgctcaccgt gctggggctg
     2881 cagatcggca cgctcctcgg cggggccgtc atcaccgagt cggtgttcgc ctggcccggc
     2941 atgggcaagc tggtggtgga cgcgatcttc ttccgcgatt tcccggtggt gcagacggtg
     3001 ctgatcctgt ccgccaccat cttcgtggtg atcaatttgc ttgtggacct gctctacacc
     3061 gtgatcgatc cccggatccg ctactcgtga gcgcgccggt cgcggccgag gcggcgcggc
     3121 acagcggccg cttgagcccg cccgccctcg tgggcggcgt gctcctggcc ctggtggtgc
     3181 tggcgagcgt cctcgccccc ctgctcgcgt cgagcgatcc ggtgaagccg tccttcagcc
     3241 gccgcctcgc gccgccgtgg ggcctggggg gcaccccggc gcacgtgctc ggcaccgaca
     3301 acctggggcg cgacatcctc gcgcgcgtgc tgcacggcgg gcggatctcg ctggcgctcg
     3361 ccacggtctc ggtgctcatc gccgcgctgg tgggcatcct ggtgggcctg gtggcgggct
     3421 cggccggcga gcggctcgac ggcctgctga tgcgggcggc ggacgtgcag ctcgcgttcc
     3481 cggtgatcat gctcgccatc gcgatcgtgg cggtggtcgg cacgagcccg ggcgcactgg
     3541 tcggcgtgct cgcgctgagc ggctgggtgc tctatgcgcg cacggtgcgc gcgagcgtgc
     3601 tcaccatccg ccgcctcgag tacgtggagg ccgcctacac cctgggggcc agcgatctcc
     3661 ggatcatcgc ccgccacatc ttgcccaaca cgatcgcgcc gatcctggtg ctcggctcga
     3721 gccagttcgc gaccatggtg ctgctcgagt cggggctcag ctttctcggg atgggcatcc
     3781 aggcgccccg gccctcgtgg gggctgatgc tggccgaggg gcgggactat ctctcgaacg
     3841 cgtggtggct ggctacgatt cccggcgtcg cgatctcgct ggtggtgctg ggagcgaacc
     3901 tgctgggcga cgggctgcgg gacctgctgg atccgcgggg gtagctcctc accctgtccc
     3961 cctcactcac tcccctcacc ctgccctctc cccagagggg agagggttag aagagagggg
     4021 gagggttagg agcggatcag gggggcgaag cgctccatcc gctccaggct ctcggagagg
     4081 ttctcggcgc ggaagtcgaa gatgagctcg ctcaccccga ggccggcgaa ggcgccgata
     4141 tcgtcggcga tggcctgctg gctccccgag aacggccgcc gctccgcgcc cccgtccact
     4201 ccctgcgccg gatcgtagat cggcgccttg taggagatcg tgagggtcga gaagtcgcga
     4261 cgctccgcct cggtgaggcg gcgcaggtcg tcgagctccg cacgcagctc ggccggccgc
     4321 agcgggacgg ccgggttggc gcccaccgga tgccagccgt cgcccaggcg cgcgacccgg
     4381 cgcagggcgg ccttgctgtg gccgccgatc cagatcggcg ggtgcggctt ctgcgccggg
     4441 tgcggcaggc agtggatgcg gtcgaagcgg tagaactcgc cgtggaacgt ggcgggatcc
     4501 tgcgtccaca gggtcttgaa gatgcggagg tactcgtcgc tcaccgcccc gcgccggtcg
     4561 aagtcgggcg cgcccagggc ctggaactcc tcccgcagcc agccgacccc gacgcccacc
     4621 gtgacgcgcc cgcgggagag cacgtcgatg gtggcgagca tcttggcggt caccaccgga
     4681 ttgcggtgcg gcaggatcat cacgctgctg atcaggcgca gccgccgcga cttgcccgcg
     4741 acgaaggcca tgagcgagag ctgctcgaga gcggcaccct ggcccgggaa ggcc
//
LOCUS       DATMCV010000071         3653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_603485, whole genome shotgun sequence.
ACCESSION   DATMCV010000071 DATMCV010000000
VERSION     DATMCV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603485"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VNU03_01740"
     CDS             <1..754
                     /locus_tag="VNU03_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73198.1"
                     /translation="RHLRVYMRNWHTAFLPPAVEPVTMLVAFGVGLGGYVATLTWQGR
                     PIEYMTYVAPGLLAYTTFMTAIFQSLFAAFIRMRYQRTWEGQLTTQVELTHVVWGEVL
                     WAGLLATMYVVMVSAVLVVIQWAGLLALDLRWLPVLWPISFVAGCGFAALGLCFTAIV
                     PTIDHVNLPVFLLVLPMGMLSSTYFPLEHPVLVALNAGNPLYHLAQAFRGILIGGPVA
                     GHLIGVLGLSALMLAILVPLDLRLLRRRVLGG"
     gene            complement(756..1820)
                     /locus_tag="VNU03_01745"
     CDS             complement(756..1820)
                     /locus_tag="VNU03_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73199.1"
                     /translation="MLARLGRAAALAGLLVLPDPAWAADAPASEEGQALDVRRLAPEQ
                     RRRLLAGDTIAYQVAETSETELGAGVAMYVAVPLSRAAEVLTSADVVLKDPSIIASGH
                     IPAGATVAALHGFKLSAAEIGEAQDALDVAAGSRFNMTLPEINLFRAAKGTVRANDRA
                     AVLDAATGQWRTLLLERAQAFQTRGLDGVTPYARRSGTSDPAALLRVAAGDARIVSSL
                     APRLGEALLRYPADQSPTAISQVYWVKRQVQGRPTPLLLHHLVDVTPALALYVERQFY
                     VGHTYNASQILCAAVPYEDGVLILSSNRVSTDQVAGLGGELKRTIGRRQLRGEIVKRF
                     DRIRAALVRPTGPPERVESP"
     gene            complement(1830..2156)
                     /locus_tag="VNU03_01750"
     CDS             complement(1830..2156)
                     /locus_tag="VNU03_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73200.1"
                     /translation="MSSSPPLPDLLREQDARHPVWLRLIFLAAAVVCLAAGVVGWLIP
                     VITGIPFYAAGLVFLGLASDRVRRWINRLERRLAESTRRRIRRLLAKLPGAWVRRLIH
                     IPDEVA"
     gene            2275..2814
                     /locus_tag="VNU03_01755"
     CDS             2275..2814
                     /locus_tag="VNU03_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73201.1"
                     /translation="MPESVPSSLIIDTFKEIFQAERRGAKRQDPLGWIISVPDTHQKS
                     VLAALDKERSLRVLTCSTEDDANAVAAGLYMTGEPVIVMIQHAGLYASVNTIRGISMD
                     GKIPIFYMIGLLSREKDKDPKDSRHSMVRFAEPLLDVFSIPHARMEGPDDVKLIPEYY
                     RKSRERKGPAAVLVGLETA"
     gene            2814..3389
                     /locus_tag="VNU03_01760"
     CDS             2814..3389
                     /locus_tag="VNU03_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWP73202.1"
                     /translation="MMKPEEILTLVAAARGTAICVPTMTTSPAWRTIAPDDLSVGCVG
                     FMGGASALGLGLALGAPDRRVIVFDGDGSLLMQLGSLATIAGAAPRNFTHLLFKNGVY
                     HTSGSQETPGGQGVDFAVMAKAAGYRSTCTITSLDEFKKRLPGLLSEEGPIMAQLITG
                     LAGSTPMNSPPGTPFHQQVANVHAKVAGGRG"
     gene            complement(3447..>3653)
                     /locus_tag="VNU03_01765"
     CDS             complement(3447..>3653)
                     /locus_tag="VNU03_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP73203.1"
                     /translation="RRGVNYGSNRVRFTAPVPAGARVRLRQKLLKVEPVDDNGFRVTS
                     EMTMEVEGNTRPAMVAETLGIIYA"
BASE COUNT          511 a         1271 c         1322 g          549 t
ORIGIN      
        1 ccgccacctg cgcgtctaca tgcgcaactg gcacaccgcg ttcctgccgc ccgcggtgga
       61 gccggtcacc atgctggtcg cgttcggggt cgggctcggc ggctacgtgg cgacgctgac
      121 ctggcagggt cgcccgatcg agtacatgac ctacgtggcg ccgggcctgc tcgcctacac
      181 gaccttcatg accgcgatct tccagtcgct cttcgccgcc ttcatccgca tgcgctacca
      241 gcgcacgtgg gagggccagc tcaccaccca ggtcgagctg acccatgtgg tgtggggcga
      301 ggtgctgtgg gcgggcctgc tcgcgaccat gtacgtggtg atggtgtccg cggtgctggt
      361 cgtcatccag tgggcggggc tgctcgcgct ggatctacga tggctgccgg tgctgtggcc
      421 gatcagcttc gtggccggct gcgggttcgc ggccctcggg ctctgcttca ccgcgatcgt
      481 gccgaccatc gaccatgtga acctgccggt gttcctgctc gtgctgccga tggggatgct
      541 ctcgtcgacc tacttcccgc tcgagcaccc ggtgctggtc gcgctgaacg cgggcaaccc
      601 gctctatcac ctggcccagg ccttccgcgg catcctgatc ggtgggccgg tcgcggggca
      661 cctgatcggc gtgctcggcc tgtccgcgct gatgctcgcg atcctggtcc cgctcgacct
      721 gcgcctgctg cgccggcgcg tgctgggcgg ctaggctacg ggctctccac ccgctcgggc
      781 ggcccggtcg gccgcaccag cgcggcgcgg atgcggtcga agcgcttgac gatctcgccg
      841 cggagctgcc gccgcccgat ggtgcgcttc agctcgccgc cgaggccggc cacctggtcg
      901 gtggacacgc ggttgctcga gaggatcagc acgccgtcct cgtagggcac cgcggcgcag
      961 aggatctggc tcgcgttgta cgtgtggccc acgtagaatt gccgctccac gtagagcgcg
     1021 agcgccgggg tcacgtccac caggtggtgg agcagcagcg gggtgggccg gccctggacc
     1081 tggcgcttca cccagtacac ctggctgatc gcggtcggac tctggtcggc cggatagcgc
     1141 agcagggcct cgccgaggcg cggggccagc gacgagacga tgcgcgcgtc ccccgcggcc
     1201 acgcggagca gcgcggccgg atcgctcgtg ccgctgcggc gcgcgtaggg ggtgacgccg
     1261 tcgaggccgc gcgtctggaa ggcctgcgcg cgctccagca ggagggtgcg ccactgcccg
     1321 gtcgccgcgt ccagcaccgc ggcgcggtcg ttggcccgca ccgtcccctt ggccgcgcgg
     1381 aagaggttga tctcgggcag cgtcatgttg aaccgcgagc ccgcggcgac gtcgagcgcg
     1441 tcctgcgcct ccccgatctc ggccgcggag agcttgaagc cgtggagggc ggccacggtg
     1501 gcgcccgcgg ggatgtgccc cgaggcgatg atgctcggat ccttcaggac gacgtcggcg
     1561 ctggtgagga cctcggccgc gcgcgagagc gggaccgcca cgtacatcgc gacgcccgcg
     1621 cccagctcgg tctcgctggt ctcggccacc tggtaggcga tcgtatcccc ggcgaggaga
     1681 cggcggcgct gctccggggc gagacggcgg acgtcgaggg cctggccttc ctccgacgcc
     1741 ggggcgtcgg cggcccaggc tgggtccggc aggacgagca gtcccgcgag ggcggccgcc
     1801 cggccgagtc gagcgagcat gaagctatct taggcgactt cgtccggaat atggatcagc
     1861 cggcgcaccc aggcccccgg cagcttggcg agcaggcggc gaatcctccg ccgcgtgctc
     1921 tcggcgagcc ggcgctccag gcgattgatc cagcgccgga cccggtcgct cgcgaggccc
     1981 aggaacacga ggcccgcggc gtagaacggg atgccggtaa tgaccgggat gagccagccg
     2041 accacgccgg ccgccaggca cacgaccgcg gcggccagga agatcagccg cagccaaacc
     2101 gggtgacgcg cgtcctgctc ccggagaaga tccggcagtg ggggtgagga gctcatcggg
     2161 aatacaggca gggtaccacg cgccaccaga tgaaccgctt tcgcgagggc cgcatcctgt
     2221 ggcagactct ccgctggtcc agcggagcag ctgcgtattc ccaggaggag cccgatgccc
     2281 gagtccgttc cgtcctctct gatcatcgac acgttcaagg agatcttcca ggccgagcgt
     2341 cggggcgcca agcgccagga cccgctcggc tggatcatca gcgtgcccga cacgcaccag
     2401 aagagcgtgc tggccgccct cgacaaggag cggagcctgc gggtgctcac gtgctcgacc
     2461 gaggacgacg ccaacgcggt ggcggccggg ctctacatga ccggcgagcc ggtgatcgtg
     2521 atgatccagc acgccggcct ctacgcgagc gtgaacacca ttcgcggcat ctcgatggac
     2581 ggcaagatcc cgatcttcta catgatcggg ctgctgagcc gcgagaagga caaggatccg
     2641 aaggactcgc ggcattcgat ggtgcgcttc gccgagccgc tgctcgacgt gttcagcatc
     2701 ccccacgccc gcatggaggg gccggacgac gtgaagctga tccccgagta ctaccggaag
     2761 agccgcgagc ggaagggccc cgccgcggtc ctcgtcggcc tggagaccgc gtgatgatga
     2821 agccggagga gatcctgacc ctcgtcgcgg ccgcgcgcgg gaccgcgatc tgcgtgccca
     2881 ccatgaccac ctcccccgcg tggcgcacga tcgctcccga cgacctctcg gtgggctgcg
     2941 tcggcttcat gggcggcgcc tccgcgctgg ggctcggcct cgcgctgggc gcgcccgatc
     3001 gccgggtgat cgtcttcgac ggcgatggct cgctcctgat gcagctcggc tcgctcgcca
     3061 ccatcgcggg cgcggccccg cggaacttca cgcacctgct gttcaagaac ggcgtgtacc
     3121 acacgtccgg ctcgcaggag acgccgggcg ggcagggggt ggacttcgcg gtgatggcga
     3181 aggcggcggg ctaccgcagc acgtgcacga tcacgagcct ggacgagttc aagaagcgcc
     3241 tgcccggtct cctctccgag gaggggccga tcatggcgca gctcatcacc ggcctcgcgg
     3301 gcagcacgcc gatgaactcg ccgccgggta cgccgttcca ccagcaggtg gcgaatgtgc
     3361 acgcgaaggt ggcggggggg cgggggtagc gcctcagcct gggccgctca ccctgccctc
     3421 tcccccgggg ggagaggggg agggggttag gcgtagatga tgcctagcgt ctcggcgacc
     3481 atggcggggc gcgtgttgcc ctccacttcc atggtcatct cgctggtcac gcggaagccg
     3541 ttgtcgtcga ccggctccac cttgagcagc ttctggcgca gccggacgcg cgcgcccgcg
     3601 ggcaccggcg cggtaaagcg cacgcggttg gatccgtagt tgaccccgcg gcg
//
LOCUS       DATMCV010000072         9056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_469178, whole genome shotgun sequence.
ACCESSION   DATMCV010000072 DATMCV010000000
VERSION     DATMCV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469178"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3555
                     /locus_tag="VNU03_01770"
     CDS             <1..3555
                     /locus_tag="VNU03_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWP73204.1"
                     /translation="PFSEQRGGVLKLKLDVSDDLDADNVAYAVIPAPRTLSVLLVSPG
                     NLFLEKVLHTDPQVKLEVRKPDAYQGGMEGFDVVVVDSVSPAKLGNGRFVLVNTVPAD
                     VPLEVLGRLDNPTIMDWDRTHPIMRQIDFAKVTIEDAMRVRPVAAGKTLVEAVGGPLI
                     YALEERDRKALFFGFDLFRTDFPLRVAFPLILSKGLRWLSPVGLDQSSLQLQAGQPIL
                     LPVEHGVTTATVRTPSGRSVRAQVNRGMASFTDTDEVGVYTVVTGRGETKVAVNLMNA
                     DESDLTPQPVPTFVEGARPDSPPVLIQRELWPFFVMLALLLFAVEGFLYWRRQTGGRL
                     GLPRGLGDRWALGLRCALLAVLLLSLTKPTIPRWADRLNVTFLLDVSDSVSLAARESG
                     YRFAAQSVAAMQPGDQAGVVVFGEEAMVDQPLRPTSKIERPQAHVPGRGTNLAQALQL
                     GLATAPPGQANRFVLLTDGRQNMGNALAVAQAAKDAGADIYYVPAPLTFPQEVVVESM
                     VLPQEVKFGEPFQAKVVAWSQAEAQGRLSLYRNGEFLGSQVVRLAPGKNVYTYRQSLE
                     QSGIHVYQAALDVEGDTIEENNRALGTLVVRGRPQVLLAEKDRAQAQALAAALRSQHV
                     DVTVVEADKIPKDMAGLQKYDGLVLSNVSSLKMTKKQMENIRDYVREQGGGLIMLGGE
                     ESFGLGGYYRTPIEEALPVTMEVKQKIEIPSLAVVLSIDRSGSMAMTTDEKVTKLDIA
                     KEAAHLVVDLLDERNEVGVMSWDTEFIWDSPLKPARDKQSIHHAIATIKAGGGTDGYP
                     ALKESYQVLFDRPALLKHVIFLSDGQMTRGDFSGLLRRMAKDKITVSTVAIGKDADVP
                     LMVDVAKWGRGRFYYTEDDSTIPRIFTLETQLASKASLVEQPFKAVVASPAHEAIQEI
                     DWKEAPPLGGYVATSMKANADLVLMTHQEDPLLATWRYGLGRSVAFTSDAKAKWGVLW
                     VRWGGFNKFWSQVTRWALRTGTRSDTVATVSRTDNVGEVTVDAIDPKGEFINFLDSQV
                     GVVAPDKTRTVIDLEQVGPGRYRGRFPAGQEGVYLVGMAQRRADQMVGSQLAGLVVPY
                     GQEFRDLGVDESFLRELTELTGGGLLSEPKDAFLQNRHRSRLSVDIWPWLVGMVAMLL
                     IPEIALRRIGPVGLGRLWTRMRVGRAKGGPGHESADQR"
     gene            3533..4876
                     /locus_tag="VNU03_01775"
     CDS             3533..4876
                     /locus_tag="VNU03_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP73205.1"
                     /translation="MRALTNARWAVVPALLLALLGAGGAEAASKPRNVLILPFTTVDL
                     TRDEQWIGEGIAQSLMLALVHVPGLVQIDRERVRALPQPEAWDETTTASAAKQLGADI
                     ALFGEVKRTGSDLIVQPRFLELRGDKVERGALDTFAVAEGQLLDRLRPLPAAYLKALK
                     APASDVELARAQKWAAPTSVARAYELYVRGRHASYRGTQDASEAAVEFLSRAIEADPQ
                     FVAAQYALGVVHQNLGNRWKAAAQFRASAQLDPTYAEPYKALGDLYLTAPRRLFEEAI
                     KAYAKAIELRPFYAEAHVGLGDALAAKGEVDPAVAAYQKGLEYNPMNAKVHVSLGKIY
                     YSEKGLYYESVTAYKKAIDLDPGYLDARMGLAEVYEDKGLYKEAIEEYRKVVDADAKN
                     TGALYNLALVYEKVDPKEAVNLWERYIGLAGSQPSEKDWVDVARLHLKKLKQQGN"
     gene            complement(4873..5055)
                     /locus_tag="VNU03_01780"
                     /pseudo
     CDS             complement(4873..5055)
                     /locus_tag="VNU03_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            5346..7535
                     /locus_tag="VNU03_01785"
     CDS             5346..7535
                     /locus_tag="VNU03_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP73206.1"
                     /translation="MSAFFLLFLVLGGLLRLPAWSGNLPLFALYWLATTVIFLVSRRS
                     ERIVLASGMAVALFDAPIIFFLQWATFPTSNPSGVAGFTVGVYVLLVMLAAFSLQHWY
                     VVFTAVVVVVYEVLLQHFAGVSVGAMVSTVILIGLAAAVCSYAQYRLLRFVRRVDRHI
                     RAHREAEAARRQAERVSALADLAHELSGTLDPTTVAERTVDSIVRLLGARTAILFRLD
                     PASGDLVTVATIGIDRQSFPLGTRLAAGAGATGQAVLRREVVITADVLAEPGISMPPD
                     LEARLLESNHRAACAVPLIVRGTVIGALRVADVAGRPFTDDEVHLAQAFADHAAFSLE
                     NARLYAELDAHLRQLETTQQQLVQAGKLAAVGQLVTGIAHEINNPLATIMGQAEMLSR
                     RLSDAGQLERVTKIADCAMRAATIVRGLQTFVRPQPHEVAALDLRDVVARVTSLRRDA
                     MRFNGVELIEDLPEGVPLVLGDAGQLEQVLLNLVINAEQAVAAVTSAPRIAISLSVHD
                     SRVRLSVADTGPGIPADILPRIFEPFFSTKAPNQNSGLGLSISYSIVQNHGGRLIAES
                     VAGNGATFAIELPAHRGPVPAAPRPDPESPPLRRGTVLVVEDEPHVAGMLRDLLTDLG
                     LDVSVIADAESAWRLLAEDARDFDAITVDLRLSGLSGRALFERLERELPRLAARVIFI
                     TGEIADADSEAFLERSGRPTLRKPFSVKALIASLATLLARGPASMRR"
     gene            7855..8244
                     /locus_tag="VNU03_01790"
     CDS             7855..8244
                     /locus_tag="VNU03_01790"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWP73207.1"
                     /translation="MIRRSSGRERGFTLVELMVVVAVIGVLATIAIPLFANVQARGRL
                     AKAQADSRTLASAVSIYGAHMGRIPSTLAQLTSVSTNALGQPGGPFMAQVPIPPGGWS
                     SYTYSANVAAGTFTISTSGDGTSVALP"
     gene            8329..9048
                     /locus_tag="VNU03_01795"
     CDS             8329..9048
                     /locus_tag="VNU03_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73208.1"
                     /translation="MSDLRKDPTRGRWVLVRPGGKPPASSACPFCPGNEALTPHEIFA
                     YRKEGSRSDGPGWSVRVIPEADPYFRIEQELVREGVGLYDRISPRGATELIVESPNHD
                     DTAASLGDGQWEQVLWMYGERIRDLKRDPSIRDILITRRHRAHGSRITHPYSRLTAIP
                     IIFDEVRRKLRECREHYEYKRRCVYCDMIRQEIAAGDRVVRLTDHFLALSPYAACSPY
                     ETWILPRQHNCLYEGSLSGRW"
BASE COUNT         1381 a         3131 c         3151 g         1393 t
ORIGIN      
        1 ccgttcagcg agcagcgggg cggcgtcctc aagctgaagc tcgacgtctc cgacgatctc
       61 gacgccgaca acgtggccta cgcggtgatc ccggcccccc ggaccctctc ggtgctgctg
      121 gtcagcccgg gcaacctgtt cctcgagaag gtcctccaca ccgatcccca ggtgaagctc
      181 gaggtccgca agccggacgc gtaccagggc gggatggaag gcttcgacgt ggtggtggtc
      241 gacagcgtga gcccggccaa gctcggcaat gggcggttcg tgctcgtcaa caccgtgccc
      301 gccgacgtgc cgctcgaggt gctgggccgc ctcgacaacc ccaccatcat ggactgggat
      361 cggacgcatc cgatcatgcg ccagatcgac ttcgccaagg tgacgatcga ggacgcgatg
      421 cgcgtccggc cggtcgccgc gggcaagacc ctggtggagg cggtgggcgg cccgctgatc
      481 tacgccctcg aggagcgcga tcgcaaggcg ctcttcttcg ggttcgatct cttccgcacc
      541 gacttcccgc tgcgggtggc cttccccctg atcctctcca aggggctccg ctggctgagc
      601 ccggtcggcc tcgaccagtc gagcctgcag ctgcaggcgg gccagcccat cctgctgccg
      661 gtggagcacg gcgtgaccac cgccaccgtg cgcaccccgt cggggcgcag cgtgcgggcc
      721 caggtcaacc ggggcatggc gagcttcacc gacaccgacg aggtgggcgt ctacacggtc
      781 gtgaccggcc gcggcgagac caaggtcgcg gtgaacctga tgaacgccga cgagtcggat
      841 ctcaccccgc agccggtgcc gaccttcgtg gaaggggcgc ggcccgactc gccgccggtg
      901 ctgatccagc gtgagctgtg gccgttcttc gtcatgctgg ccctgctcct gttcgcggtc
      961 gaggggttcc tctactggcg gcgccagacc ggtggccggc tcgggctgcc gcggggcctg
     1021 ggcgaccggt gggcgctcgg tctgcgctgc gcgctcctcg cggtgctcct cctgtcgctg
     1081 accaagccga cgatcccgcg ctgggccgac cgcctgaacg tcacgttcct gctcgatgtg
     1141 tcggacagcg tgagcctggc cgcgcgcgag agcgggtacc gcttcgcggc ccagtcggtc
     1201 gccgcgatgc agccgggcga ccaggcgggt gtggtggtgt tcggggagga ggcgatggtg
     1261 gaccagccgc tgcgcccgac ctcgaagatc gagcggccgc aggcgcacgt ccccgggcgc
     1321 ggcaccaatc tcgcgcaggc cctgcagctc ggactggcca ccgcgccgcc cggccaggcc
     1381 aaccgcttcg tgctcctcac cgacgggcgc cagaacatgg gcaacgccct cgcggtcgcc
     1441 caggcggcca aggacgcggg cgccgacatc tactacgtgc cggcgccgct caccttcccg
     1501 caggaagtgg tggtggagtc gatggtgctg ccccaggagg tgaagttcgg ggagccgttc
     1561 caggccaagg tggtggcctg gagccaggcg gaggcccagg ggcggctctc gctctatcgc
     1621 aacggggagt tcctcggctc gcaggtggtg cgcctcgccc ccggcaagaa cgtctacacc
     1681 taccgccagt cgctcgagca gagcggcatc cacgtctacc aggccgcgct cgacgtggag
     1741 ggcgacacca tcgaggagaa caaccgtgcg ctcggcaccc tggtggtgcg cgggcggccg
     1801 caggtgctgc tcgccgagaa ggaccgcgcg caggcccagg ccctggccgc ggccctccgc
     1861 tcgcagcacg tggacgtgac agtggtggag gcggacaaga tccccaagga catggcgggc
     1921 ctgcagaaat acgacggcct cgtgctctcc aacgtctcct cgctcaagat gacgaagaag
     1981 cagatggaga acatccgcga ctacgtccgc gagcagggcg gcggcctcat catgctcggt
     2041 ggcgaggaga gcttcggcct cggcggctac taccggaccc cgatcgagga agcgctgccg
     2101 gtgacgatgg aggtgaagca gaagatcgag atcccgagcc tcgcggtggt gctctcgatc
     2161 gaccgctccg ggtccatggc catgaccacc gacgagaagg tgaccaagct cgacatcgcc
     2221 aaggaggccg cccacctggt ggtcgatctc ctcgacgagc gcaacgaggt cggggtcatg
     2281 agctgggaca ccgagttcat ctgggactcg ccgctcaagc ccgcccgcga caagcagtcc
     2341 atccatcacg cgatcgccac catcaaggcg gggggcggca ccgacggcta cccggccctg
     2401 aaggagtcgt atcaggtcct cttcgaccgg cccgccctgc tcaagcacgt catcttcctc
     2461 tccgacggcc agatgacccg cggcgacttc tcggggctcc tgcgccggat ggccaaggac
     2521 aagatcacgg tgtccacggt ggccatcggc aaggacgcgg acgtgcccct gatggtcgac
     2581 gtggccaagt ggggccgcgg gcgcttctac tacaccgagg acgactccac gatcccgcgc
     2641 atcttcaccc tggagacgca gctcgcctcc aaggcgtcgc tcgtcgagca gccgttcaag
     2701 gcggtggtgg cgagcccggc tcacgaggcg atccaggaga tcgactggaa ggaggcgccg
     2761 ccgctgggcg gctacgtggc caccagcatg aaggccaacg cggacctggt gctgatgacc
     2821 caccaggaag acccgctgct cgccacctgg cgctacggcc tcggccgctc ggtggccttc
     2881 acctccgacg ccaaggccaa gtggggcgtg ctctgggtgc gctggggcgg cttcaacaag
     2941 ttctggtcgc aggtgacgcg gtgggcgttg cgcaccggga cccgcagcga tacggtggcc
     3001 acggtctccc ggaccgacaa cgtcggcgag gtgacggtgg acgcgatcga tcccaagggg
     3061 gagttcatca acttcctgga ctcccaggtc ggggtggtcg ctccggacaa gacccggacc
     3121 gtgatcgatc tggagcaggt cgggccgggc cggtaccgcg gtcgcttccc ggccggacag
     3181 gagggggtgt acctggtcgg catggcgcag cggcgcgccg accagatggt ggggtcgcag
     3241 ctcgcgggcc tcgtggtgcc gtacggccag gagttccgcg acctgggggt ggacgagtcc
     3301 ttcctgcggg agctgaccga gctgaccggg ggcgggctgc tgagcgagcc gaaggacgcg
     3361 ttcctccaga accggcaccg gtcgcggctg agcgtggaca tctggccgtg gctggtgggc
     3421 atggtggcga tgctgctgat tccggagatc gcgctgcggc ggatcgggcc ggtggggctc
     3481 ggtcggctgt ggacgcggat gcgggtgggc cgggcaaaag gaggaccagg acatgagagc
     3541 gctgaccaac gctaggtggg ccgtcgtccc cgcgctgctg ctcgcgctcc tcggcgcggg
     3601 gggtgcggag gccgcgtcca agccgcgcaa cgtcctgatc ctgccgttca ccaccgtgga
     3661 cctcacccgt gatgagcagt ggatcggcga gggcatcgcg cagtcgctga tgctcgcgct
     3721 cgtgcacgtg cccggcctgg tccagatcga ccgcgagcgg gtccgcgcgc tcccgcagcc
     3781 ggaggcgtgg gacgagacga ccaccgccag cgcggccaag cagctcggtg ccgatatcgc
     3841 gctcttcggc gaggtcaagc ggaccggctc ggacctgatc gtccagccgc gcttcctcga
     3901 gctgcggggg gacaaggtcg agcggggcgc cctcgacacc ttcgcggtcg cagagggcca
     3961 gctcctcgac cgcctgcgcc cgctgcccgc cgcctacctc aaggcgctga aggcccccgc
     4021 cagcgacgtg gagctggcgc gcgcccagaa gtgggccgcc ccgacgagcg tcgcgcgcgc
     4081 gtacgagctg tacgtgcggg gccgccacgc gagctatcgc ggcacccagg acgcgagcga
     4141 ggccgcggtg gagttcctga gccgggccat cgaggcagac ccgcagttcg tggcggccca
     4201 gtacgccttg ggagtggtgc accagaacct cggcaatcgt tggaaggcgg cggcccagtt
     4261 ccgggcctcg gcccagctcg atccgaccta cgcggaaccc tacaaggcgc tcggggatct
     4321 ctacctgacc gcgccgcgcc ggcttttcga ggaggcgatc aaggcctatg ccaaggccat
     4381 cgagctccgg ccgttctacg cggaggcgca cgtcggcctc ggcgacgcgc tcgcggccaa
     4441 gggcgaggtg gacccggcgg tggccgcgta ccagaagggg ctcgagtaca acccgatgaa
     4501 cgccaaggtg cacgtgagcc tgggcaagat ctactactcg gagaaggggc tctactacga
     4561 gtcggtcacc gcctacaaga aggcgatcga tctcgacccg ggctacctcg acgcgcggat
     4621 gggcctggcc gaggtgtacg aggacaaggg cctctacaag gaggccatcg aggagtaccg
     4681 caaggtcgtg gacgcggacg ccaagaacac cggcgcgctc tacaacctgg ccctcgtcta
     4741 cgagaaggtc gacccgaagg aggcggtcaa cctctgggag cgttacatcg gcctggccgg
     4801 cagccagccc tcagagaagg actgggtcga cgtcgcgcgc ctgcacctga agaagctcaa
     4861 gcagcagggg aactagagcc cgagcaggct cttgagcgcg ggcttgttga agccgcgcac
     4921 cacctgatcg ccgaccacga tcaccgggat cgacatctgc ccggtgagct tgatcagctc
     4981 gtcgcgtgcg tcggcatccg ccagcacgtt gatatcctcg aacgccacgc cctgagacga
     5041 aagatactct ttcgccgcct tgcagggccc tcaggtgggg ctgctgaaca cccggacctt
     5101 cgccgtctgg tcgctcatgc cccaagggta gcacagggag tccggggcgg ccccgcgggc
     5161 ctacgtaggg atacctagcc cgaaccgggc cgccggctcc cggcccgaat gaagccctcc
     5221 actgggccgg gagatatact cgcggcgtgg aggagtctca cggatcccgt gattcgctcc
     5281 aacgcgagct gacccgcgaa cgtctgaaaa acgcccgccg cagcaacgcg gttcgcttct
     5341 ggggagtgtc cgcgttcttc ctgctgttcc tcgtgctggg cggcctgctc cggctcccgg
     5401 cgtggtccgg caatctgccg ctcttcgcgc tgtactggct cgccaccacc gtgatcttcc
     5461 tggtcagccg gcgttccgag cgcatcgtgc tcgcctccgg catggcggtc gcgctcttcg
     5521 acgcgccgat catcttcttc ctccagtggg cgacgttccc gaccagcaac ccgagcgggg
     5581 tcgccggatt cacggtcggg gtctacgtgc tcctcgtcat gctcgccgcg ttctccctgc
     5641 agcactggta cgtcgtcttc accgcggtgg tggtggtggt gtacgaggtc ctcttgcagc
     5701 acttcgccgg ggtgagcgtc ggcgcgatgg tctccaccgt catcctgatc ggcctcgccg
     5761 ccgcggtctg ctcctacgcg cagtatcggc tcttgcgctt cgtccggcgc gtcgaccggc
     5821 acatccgcgc ccaccgggag gccgaggcgg cgcgccggca ggccgagcgg gtctcggctc
     5881 tggccgatct cgcccacgag ctttccggga ccctcgatcc gaccacggtg gccgagcgga
     5941 cggtcgacag catcgtccgc ctgctcgggg cccgtaccgc gatcctgttc cggctcgatc
     6001 ccgcgtcggg cgatctggtg acggtcgcca ccatcgggat cgaccgtcag agcttcccgc
     6061 tcggcactcg ccttgccgcg ggagcggggg cgaccggaca ggcggtcttg cggcgagagg
     6121 tcgtgatcac ggcggacgtt ctggccgagc ccggcatctc gatgcccccc gacctggagg
     6181 cccggctcct cgaatccaat caccgcgcgg cgtgcgcggt tcccctgatc gtgcgcggta
     6241 ccgtcatcgg cgcgctccgg gtggcggacg tggcgggacg acccttcacc gacgacgagg
     6301 tccatctcgc ccaggccttc gcggaccacg ccgcgttctc gctcgagaat gcgcgcctct
     6361 acgccgagct ggacgcgcat ctccgtcagc tcgagacgac gcagcagcag ctcgtacagg
     6421 cgggcaagct cgcggcggtc ggtcagctcg tgaccggtat cgcgcatgag atcaacaatc
     6481 cgctcgcgac catcatgggg caggccgaga tgctgagccg ccgcctctcg gatgcgggcc
     6541 agctcgaacg ggtgaccaag atcgccgatt gcgcgatgcg cgcggccacg atcgtgcgcg
     6601 gcctgcagac gttcgtccgc ccgcagccgc acgaggtggc ggccctcgac cttcgcgacg
     6661 tggtggcgcg ggtgacctcc ctgcgccggg acgcgatgcg gttcaacggg gtcgagctga
     6721 tcgaggatct tccggagggc gtgccgctcg tgctgggcga cgcggggcag ctcgagcaag
     6781 tcctgctcaa cctggtgatc aacgccgagc aggcggtagc cgcggtcacc tcggcgcccc
     6841 gcatcgcgat cagtctgagc gtgcacgact cgcgggtgcg gctgagtgtc gccgacaccg
     6901 gacccgggat ccccgccgac atcctgccgc gcatcttcga gccgttcttc tcgacgaagg
     6961 cgcccaacca gaactcgggg ctgggcctgt ccatctccta cagcatcgtc cagaaccacg
     7021 gggggcggct catcgccgag agcgtcgcgg gcaacggggc gaccttcgcg atcgagctgc
     7081 ccgcgcatcg gggcccggtc cccgccgcgc cgcgcccgga cccggaaagc ccgccgctgc
     7141 gtcgcggaac cgtcctcgtg gtcgaggacg agcctcacgt cgcgggtatg ctgcgggacc
     7201 tcctgaccga cctggggctg gacgtgagtg tcatcgccga tgcggagagc gcgtggcggc
     7261 tcctggccga ggacgcgagg gacttcgacg cgatcacggt ggaccttcgc ctctccggcc
     7321 tctcggggcg ggcgctgttc gagcgcctcg agcgcgagct gccgaggctc gccgcccggg
     7381 tgatcttcat cacgggcgag atcgcggatg cggacagcga ggcattcctc gagcgctcgg
     7441 gccggcccac cctgcgaaag ccgttcagcg tgaaggcgct catcgcgagc ctggccacgc
     7501 tgctcgcgcg cggtccggcc tcgatgaggc ggtgagcgtc ggctcggcca gcgcgcgaaa
     7561 gagcggccgt cgcaggaaat ctggctgtgc gaggctaggg gcatgcgccg gcgcgggtgg
     7621 atggatacgg gcgacgccat agtgcgcctc gcctggaagc tcttgtggag ctggagacga
     7681 cggtaggggg cccgggcaag acttcgccca cgataccgcc ggtttcagtc actttccctg
     7741 cctgggctcg tcagcttccg atcgtcaaga gacgtgaaat caacaagata gcccggtggc
     7801 ccctgtcttg ctccgaattg aggcatttgc ggcccaactc cggaggggac gatcatgatc
     7861 cgcaggtcat cgggtaggga gcgaggcttc acgctcgtcg agctgatggt ggtcgtggcc
     7921 gtgatcggcg tcctcgccac gattgcgatc ccgctgttcg cgaacgtgca ggcgcggggg
     7981 cggctcgcca aagctcaggc cgattcgcgc acgctggcat ccgcggtgag catctacggc
     8041 gcccatatgg ggcggattcc ctccacgctc gcccagctca cctcggtgtc gaccaatgcc
     8101 ctcggccagc cgggcggacc cttcatggcc caggtcccga tccctcccgg cgggtggtcg
     8161 agctacacct acagcgccaa cgtcgccgcc ggcaccttca ccatcagcac gagcggcgac
     8221 gggaccagcg tcgcccttcc gtagccctcg cgtttctgcc cggcgcgatc ggactcggtc
     8281 ccgcgtctcc ttgacttaac cgggggcatt tcctatggtg gccgagtcat gagcgatctg
     8341 cgcaaggatc caactcgggg tcgctgggtc ctcgtccgcc ccggcggcaa gccgcccgcg
     8401 tcgtcggcgt gccccttctg tcccggcaac gaagcgctca cgccccacga gatcttcgcc
     8461 tatcgcaagg agggctcgcg atccgacggg cccgggtggt cggtgcgggt gatcccggag
     8521 gccgatccgt acttccgcat cgagcaggag ctggtccgcg agggagtcgg actctacgat
     8581 cgcatctcgc cgaggggcgc caccgagctg atcgtcgaga gccccaacca cgacgacacc
     8641 gccgcgagcc tgggggacgg gcagtgggag caggtgctgt ggatgtacgg cgagcgcatc
     8701 cgcgatctca agcgcgatcc gagcatccgc gacattctca tcacgcggcg tcaccgggcg
     8761 cacggctcgc gcatcacgca tccgtactcg cggttgaccg cgatcccgat tatcttcgac
     8821 gaggtccgcc gcaagctccg cgagtgccgc gagcactacg agtacaagcg acgttgcgtg
     8881 tactgcgaca tgatccgcca ggagatcgcg gccggcgatc gggtggtgcg gctcaccgac
     8941 cactttctgg ccctctcgcc ctacgccgcg tgctcgccct acgagacctg gatcctgccg
     9001 cgccagcaca actgcctcta cgaggggtcg ctctcgggga ggtggtagcg gatctg
//
LOCUS       DATMCV010000073         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_936168, whole genome shotgun sequence.
ACCESSION   DATMCV010000073 DATMCV010000000
VERSION     DATMCV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936168"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VNU03_01800"
     CDS             <1..1330
                     /locus_tag="VNU03_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP73209.1"
                     /translation="LSLWKLDPVAGLRQATALVIAWLHGCVGLHFWLRLRPWYARYAR
                     AIYTGFLLLPVLALLGFAEAGREITRLAEDPAFVPRVLAAAHLPSPEQRVVLGRVSRG
                     IFWGHLGIIALVLAVRAGREHVRRRQGVRVTYPGGREVLVPAGFTILEASRFGGIRHA
                     SVCGGRGRCSTCRVRVIRGGEHLPPATAEELRVLGRVGAPAHVRLACQVRPRRDLTVV
                     PLVPASAGPGDAVGMGDHREGREATIAVLFADLRGFTRLAERKLPYDVVFILNRYFEA
                     VGGAITDVGGIVNQFTGDGVMALFGVDSGPEEGSRQAVRAAAGMVARVHELSRTLSDD
                     LAAPLRLGIGIHTGATVVGRMGYATTTYLTAVGDTVHVAARLEALTKDYNCDLVLSEE
                     VAVRAGLDVSDHARHELTVRNREAPLAIFVVPSAGRLAERLAVGGDRVGA"
     gene            1327..2115
                     /locus_tag="VNU03_01805"
     CDS             1327..2115
                     /locus_tag="VNU03_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HWP73210.1"
                     /translation="MSSHGQGGRPAGPSAPEPSFAEQARTLVHLAPSGTLGTLSRRHP
                     GHPFVSVMPYAPDDRGRPLVLISGLAMHTQNLDADPRASLLVVQAGHEDPLAMARVTL
                     MGQAQRLPAADRPAAREAYLARHPNAVHWVDYGDFAFWRLDLTDIYFVGGFGAMDWLT
                     VEGYEAARPDPLADAAPGIIEHMNRDHADALILYARVLGPVAADAAEMVAVDRLGFKL
                     RVRAGDGLEGCRIGFPREVTDTGQCRRVLIEMVEECRKRVGSGV"
     gene            2142..3017
                     /locus_tag="VNU03_01810"
     CDS             2142..3017
                     /locus_tag="VNU03_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWP73211.1"
                     /translation="MPRHIKVAAAQMGPNNEGTSREEIVERMLALLERAVAEDVEILA
                     YPEMALTTYFPKRVRDDFDQFFETEMPPKALASLLQRAREARIACHVGFCEKDQGRYF
                     NTALLTDETGHLAGTFRKIHLPGAKQPDGHAQVYEPHFFESGNSGYRVFDAAKAKVGI
                     AICQDRRFPESYRSLGLLGAEIVLIGYNTPLSPLALDLNELCLRAGAYQNNLFVVGIA
                     KAGIEDGMELIGGSCIVSPMGQVLAKAATTGDELIAARIDLDTMTPARKRWDFLGRRH
                     PEHYGLLTQPTPRGS"
     gene            3017..>3617
                     /locus_tag="VNU03_01815"
     CDS             3017..>3617
                     /locus_tag="VNU03_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73212.1"
                     /translation="MAAPHVDLIVKGGRVVTSTEVLETAVAVSGGKIVAVGAEAMLPS
                     AERTIDATGKYVLPGLMDCHVHLGAVYDDWHTGPVAAAHAGLTSLLSFVEYDDQARET
                     LPQALKRLRDEAGSQSVVDFGFHFILNNQPYILDGLAEAFEQGVSSYKIFMTYKKRPN
                     RMCSDEFIAQVMEKIAALGGVCQLHCENGDILYHLENKSI"
BASE COUNT          505 a         1298 c         1290 g          524 t
ORIGIN      
        1 cctctctctc tggaagctcg atccggtggc ggggctgcgg caggccaccg ccctcgtgat
       61 cgcgtggctg cacggctgcg tgggcctaca cttctggctg cgcctgcgtc cgtggtacgc
      121 gcggtacgcc cgcgcgatct acaccgggtt cctgctcctc ccggtgctgg ccctgctggg
      181 cttcgcggag gcgggccgcg agatcacccg cctcgccgag gatcccgcgt tcgtcccgcg
      241 cgtgctcgcg gcggcgcacc tgcccagccc cgagcagcgc gtggtcctcg ggcgggtctc
      301 gcgcgggatc ttctggggcc acctggggat aatcgcgctg gtcctcgcgg tgcgcgccgg
      361 ccgcgagcac gtccgccgtc gccagggcgt acgggtgacc tatcccggcg ggcgcgaggt
      421 gctggtcccc gccggcttca ccattctcga ggcgagccgc ttcggcggga tccgccacgc
      481 ctccgtctgc ggcgggcggg gccgctgctc gacgtgccgg gtccgggtga tccgcggcgg
      541 ggagcacctg ccccccgcga ccgcggagga gctgcgcgtg ctcggacggg tgggcgcccc
      601 cgcccacgta cgcctggcct gccaggtgcg accgcgacgc gacctcacgg tggtcccgct
      661 cgtgccggcg agcgccgggc ccggcgacgc ggtcggtatg ggcgaccatc gcgagggccg
      721 cgaggccacg atcgcggtgc tcttcgcgga cctgcggggc ttcacgcggc tggccgagcg
      781 caagctcccc tacgacgtgg tcttcatcct caaccgttac ttcgaggcgg ttggcggcgc
      841 catcaccgac gtgggcggca tcgtgaacca gttcaccggc gatggggtca tggccctctt
      901 cggggtggac agcgggccgg aggaaggctc ccgccaggcg gtgcgcgcgg ccgcgggcat
      961 ggtggcgcgc gtgcacgagc tgagccggac gctgagcgac gatctggcgg ccccgctccg
     1021 cctcggcatc ggcatccaca ccggcgccac cgtggtcggg cgaatgggct acgccaccac
     1081 cacgtatctc accgcggtgg gtgacaccgt gcacgtggcg gcgcgtctcg aggccctcac
     1141 caaggactac aattgcgacc tggtgctctc cgaggaggtc gcggtgcgcg ctggcctcga
     1201 cgtctcggat cacgcccggc acgagctcac ggtgcgcaac cgcgaggccc ccctggcgat
     1261 cttcgtggtc ccgagcgcgg ggcgcctcgc cgagcgactg gcggtcggcg gtgatagagt
     1321 cggcgcatga gctcccacgg ccagggggga cggccggccg ggccgagcgc gcccgagccc
     1381 agcttcgccg agcaggcgcg cacgctggtg cacctcgcgc cctccggcac cctgggcacg
     1441 ctctcgcgcc gccatcccgg ccatcccttc gtgtcggtga tgccgtacgc gcccgacgat
     1501 cgcggccggc cgctggtcct gatcagcggg ctcgcgatgc acacccagaa cctcgacgcc
     1561 gatccgcgcg cgagcctgct cgtggtgcag gcgggccacg aggatccgct cgcgatggcc
     1621 cgcgtcacgc tcatgggtca ggcccagcgc ctgcccgcgg ccgatcggcc ggcggcgcgc
     1681 gaggcgtacc tggcgcgtca cccgaacgcg gtgcactggg tcgactacgg ggacttcgcg
     1741 ttctggcgcc tcgacctgac cgacatctac ttcgtcggcg gcttcggggc catggactgg
     1801 ctcaccgtcg agggctacga ggcggcccgg ccggatccgc tcgccgacgc cgcgcccggc
     1861 atcatcgagc acatgaaccg ggaccacgcc gacgcgctga tcctctatgc tcgcgtcctg
     1921 ggcccggtcg ccgccgacgc ggccgagatg gtcgcggtgg accgcctcgg cttcaagctc
     1981 cgcgtccggg cgggggacgg gctcgagggc tgccggatcg gcttcccgcg cgaggtgacc
     2041 gacaccgggc agtgccgccg ggtgctgatc gagatggtcg aggagtgccg gaagcgagtg
     2101 ggctccggcg tgtgaaggcc aatagccaag gagagatgcg catgccacgc cacatcaaag
     2161 tcgccgccgc gcagatgggc cccaacaacg aggggacgag ccgcgaggag atcgtcgagc
     2221 gcatgctggc cctgctggag cgggccgtcg cggaggacgt cgagatcctg gcctatcccg
     2281 agatggcgct gacgacctat ttccccaagc gggtccggga cgacttcgat cagttcttcg
     2341 agaccgagat gccgcccaag gcgctggcct cgttgctcca gcgggcgcgc gaggcgcgca
     2401 tcgcctgcca cgtgggcttc tgcgagaagg accaggggcg ctacttcaac accgcgctgc
     2461 tcaccgacga gacgggccac ctggccggga cgttcaggaa gatccatctt ccgggcgcga
     2521 agcagcccga cgggcacgcg caggtctacg agccccactt cttcgagtcc ggcaacagcg
     2581 gctatcgcgt gttcgacgcg gcgaaggcca aggtcggcat cgcgatctgc caggaccggc
     2641 gcttccccga gtcctaccgc agcctgggcc tgctgggcgc cgagatcgtg ctgatcggct
     2701 acaacacccc gctctcgccg ctggcgctgg atctgaacga gctgtgcctg cgcgcgggcg
     2761 cctaccagaa caacctcttc gtggtcggca tcgccaaggc cgggatcgag gacggcatgg
     2821 agctgatcgg cggatcctgc atcgtgagcc cgatggggca ggtgctggcc aaggccgcca
     2881 ccaccggcga cgagctgatc gcggcgcgca tcgacctcga cacgatgacg ccggcgcgca
     2941 agcggtggga cttcctcggc cggcggcatc ccgagcacta tgggctcctg acccagccga
     3001 ctccgcgggg atcctgatgg cggcccctca cgtcgacctg atcgtcaagg gcgggcgcgt
     3061 cgtcacctcc accgaggtcc tggagaccgc ggtggcggtg agcggcggca agatcgtggc
     3121 cgtaggcgcg gaggcgatgc tgccgtccgc cgagcggacc atcgacgcca ccggcaagta
     3181 cgtgctgccc ggcctcatgg actgccacgt gcacctgggc gcggtgtacg acgactggca
     3241 caccggcccg gtcgcggcgg cgcacgccgg cctcacctcg ctgctctcgt tcgtggagta
     3301 cgacgaccag gcgcgcgaga ccctgccgca ggccctcaag cggctgcgcg acgaggcggg
     3361 ctcgcagtcg gtggtggact tcggcttcca cttcatcctg aacaaccagc cctacatcct
     3421 ggacgggctg gccgaggcct tcgagcaggg cgtcagctcg tacaagatct tcatgaccta
     3481 caagaagcgg ccgaaccgca tgtgctcgga cgagttcatc gcccaggtca tggagaagat
     3541 cgcggcactg ggcggcgtct gccagctcca ctgcgagaac ggcgacatcc tctaccacct
     3601 ggagaacaag tcgatcg
//
LOCUS       DATMCV010000074         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_870045, whole genome shotgun sequence.
ACCESSION   DATMCV010000074 DATMCV010000000
VERSION     DATMCV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870045"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1534
                     /locus_tag="VNU03_01820"
     CDS             248..1534
                     /locus_tag="VNU03_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HWP73213.1"
                     /translation="MDPGRVSVARRPRQRRLALLVLLAAVGLAAAAIPFRATWWGGWI
                     LAIAEAGIVGGLADWFAVTALFRRPLGLPIPHTGIIPANWELMAERVGVMVGDRVLTK
                     DYVRGEIETFDLADLLARAAGRLRPDDLASAVRAVSQWIVLEVSPAATSDLAAWLGRL
                     ARAHPIAPILATAIEIARKQGWDQRLIEAMAEALVEALDRPDFRTAVGDLVDEVVAGY
                     RAQMSLYPRFLLGLANTLGLIDRERLVSALHDALRKIASDPGDPLRVRLSETLAALPA
                     RLRTDPDLTARLEAAKEELLASPALTRLVEDIATGLRRAIVADLEAPSSEVVDWITAR
                     LDRARLTLAEDAALRQDLDRWLKERVTEAVDRYHDRIARFIERGVHALGPEGAVRLIE
                     EHAGDDLQYIRVNGTVVGGLAGGGLYAIHLLLGLLG"
     gene            complement(1545..3272)
                     /locus_tag="VNU03_01825"
     CDS             complement(1545..3272)
                     /locus_tag="VNU03_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HWP73214.1"
                     /translation="MLYGSNLGTAEEVARRVAESGEAGGFAVTVAPLDDYVGRLPGEG
                     AVVIASASYNGTPPDNAVRFCEWLRAAEPDHVSLAGVRYTVWGCGNRDWASTFQAVPR
                     LIDERLAALGAERLYPRGEGDAREDFDGQFQAWQDPLLGTLAKALGVELAPAEAAEAA
                     HLTVEDVEGGEASPFVDSLGARAMRVAVNRELHTRTGADPSPRSTRHVELDLPDGVAY
                     RAGDHLGVIPRNADALVARVAARFGLDPAASIRLRLPGSRKTFLPVDQPVRVSRLLGD
                     YVELQDPATRKQIQGLAAHTECPWTGPRLSALAEDEARYREEVLARRKSVIDLLDEFP
                     ACQLPFNRYLEMLPPLRPRYYSISSSPLRHERRCSITVAIVEGEARSGRGTFHGVCSG
                     YLLLQKEGEPVHAFVKDTRSAFRLPDNAATPIIMIGPGTGLAPFRGFLQERAALRERG
                     QAVGPALLFFGCRHPRQDFIYEDELRAFADQGVVRLFACFSRDPGAQKTYVQDQLREQ
                     RALVWMQLQQGGTVYVCGDATRMAPDVRRAFAAIHREATGSDERAALAWLDGLVAEGR
                     YLVDVWATG"
     gene            3332..3441
                     /locus_tag="VNU03_01830"
                     /pseudo
     CDS             3332..3441
                     /locus_tag="VNU03_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          481 a         1254 c         1216 g          490 t
ORIGIN      
        1 cgcttctccc tctttgttcg atcgtttgag gattacgtga ctgccccatg acgtcgctca
       61 gcctaacacg ctgcgtcatg gtgtcaagca gaatttgatg caaagcgtta cagctatgtc
      121 cggccgcgtc atcgacctct atccgatctc tcaccctctg ctatccaaca tccattaaat
      181 gcatagataa ctatcatccg atccggcacg ctttgccgag tccgacttcc catagtagtc
      241 tccggacgtg gatcccggcc gcgtctcggt agcaaggcgg ccccgccaac gacgcctcgc
      301 cctgctcgtg ctgctggcgg cggtgggact ggcggcggcg gcgataccct tccgcgccac
      361 gtggtggggc gggtggatcc tggccatcgc cgaggccggc atcgtgggtg ggctcgccga
      421 ctggttcgcg gtgaccgcgc tgttccggcg tcccctcggg ctgccgatcc ctcacaccgg
      481 gatcatcccc gcgaactggg agctgatggc ggagcgggtg ggcgtgatgg tgggcgaccg
      541 cgtgctcacc aaggactacg tccgcgggga gatcgagacc ttcgacctcg ccgacctgct
      601 cgcgcgggcc gccgggcgcc ttcgccccga cgatctcgcc tccgcggtgc gcgcggtctc
      661 ccagtggatc gtcctggaag tctcgccggc ggcgaccagc gacctggccg cgtggctcgg
      721 ccgtctggcg cgggcgcacc cgatcgctcc gatcctggcg accgccattg agatcgcgcg
      781 caagcagggc tgggaccagc gcctgatcga ggccatggcg gaggcgctgg tcgaggccct
      841 cgaccgcccg gatttccgga ccgcggtcgg cgacctcgtc gacgaggtag tggccggata
      901 tcgggcccag atgagcctct acccccgctt tctcctcggc ctcgccaaca cccttggcct
      961 catcgaccgg gagcggctcg tgtcggcgct gcacgacgcg ctcaggaaga tcgcctccga
     1021 tccgggcgat ccgctccggg tccggctcag cgagacgctg gccgcgctgc cggcccgcct
     1081 gcgcaccgac ccggacctga ccgcccggct cgaggccgcg aaggaagagc tgctggcgag
     1141 cccggccctc acccgtctgg tcgaggacat cgcgacgggg ctgcgtcgag cgatcgtggc
     1201 ggacctcgag gccccttcct ccgaggtggt ggactggatc acggcccggc tcgaccgggc
     1261 acgcctcacc ctcgccgagg acgcggcgct acgccaggac ctcgaccgct ggctcaagga
     1321 gcgggtcacc gaggcggtgg accgctatca cgatcggatc gcgcgattca tcgagcgcgg
     1381 cgtgcacgcc ctcggccccg agggcgcggt gcggctgatc gaggagcacg cgggcgacga
     1441 cctgcagtac atccgcgtca acggcaccgt ggtgggcggc ctcgcggggg gagggctcta
     1501 cgccatccac ctcctgctgg gcctgctcgg ctgaccggtc ccggtcagcc cgtggcccag
     1561 acgtcgacca ggtagcggcc ctcggcgacg agcccgtcga gccaggccag ggccgctcgc
     1621 tcgtcggagc cggtcgcctc gcgatggatg gccgcgaagg cgcgccgcac gtcgggcgcc
     1681 atgcgggtgg cgtcgccgca cacgtagacg gttcccccct gctggagctg catccacacc
     1741 agggcccgct gctcccgcag ctgatcctgc acgtaggtct tctgcgcgcc cgggtcgcga
     1801 gagaagcacg cgaagaggcg caccacgccc tggtccgcga aggcccgcag ctcgtcctcg
     1861 tagatgaagt cctgccgggg atgacggcag ccgaagaaga gcagcgcggg gccgaccgcc
     1921 tgcccgcgct cgcggagcgc ggcgcgctcc tgcaggaagc cgcggaacgg cgccagcccg
     1981 gtaccgggcc cgatcatgat gatcggcgtc gccgcgttgt ccggcaggcg gaaggcggag
     2041 cgggtgtcct tgacgaaggc gtgaaccggc tctccctcct tctggaggag gaggtagccg
     2101 gagcacaccc cgtggaaggt cccgcggccc gagcgcgctt cgccctccac gatcgccacc
     2161 gtgatgctgc agcggcgctc gtgccggagc ggcgacgacg agatcgagta gtagcgcggc
     2221 cggagcggcg gcagcatctc gagatagcga ttgaacggga gctggcaggc cgggaactcg
     2281 tccaggaggt cgatcaccga cttgcgccgc gccaggacct cctcgcggta gcgcgcctcg
     2341 tcctcggcca gggcggagag ccgcggcccc gtccacgggc actcggtgtg agcggcgagc
     2401 ccctggatct gcttccgggt cgcgggatcc tgcagctcga cgtagtcacc cagcagccgg
     2461 ctcacgcgaa ccggctgatc aacggggaga aacgtcttgc ggctcccggg cagccggagc
     2521 cggatggacg cggcggggtc gagcccgaag cgggcggcca cccgcgcgac gagcgcgtcc
     2581 gcgttgcgcg ggatcacccc gaggtgatcg cccgcgcgat aggccacgcc gtccggcaga
     2641 tcgagctcca cgtgtcgggt cgagcgcggc gagggatcgg cgccggtccg cgtgtgcagc
     2701 tcgcggttga ccgccacccg catcgcgcgg gccccgagcg agtcgacgaa cgggctggcc
     2761 tccccgccct cgacgtcctc gaccgtcagg tgcgcggcct ccgccgcctc cgcgggcgcc
     2821 agctcgaccc cgagcgcctt ggccagggtc cccagcagcg gatcctgcca ggcctggaac
     2881 tgcccgtcga agtcctcccg cgcgtcgcct tcgccccgcg ggtagagtcg ctcggccccg
     2941 agcgcggcga gacgctcgtc gatcagccgg ggcaccgcct ggaacgtgga ggcccagtcc
     3001 cggttgccgc agccccacac cgtgtagcgc acgcccgcga gcgagacgtg gtccggctcg
     3061 gccgcgcgca gccactcgca gaagcggacc gcgttgtcgg ggggcgtgcc gttgtaggac
     3121 gccgacgcga tgaccaccgc gccctcgccg ggcagccgac cgacatagtc gtcgagcgga
     3181 gccaccgtga ccgcgaaacc gcccgcctcg cccgattccg ctacccggcg cgcgacctcc
     3241 tcggcggtgc ccaggttgga gccgtagagc accaggaggg gcgtgccggg tggcggagtc
     3301 gggtgaggcg ggcggcttcg cggtcacggt ggctccactc gacgactacg ccggtcgcct
     3361 gcccggcgag ggcgcggtgg tcatcacttc ggcgtcctac aacggcacgc cccccgacaa
     3421 cgcggtccgc ttctgcgagt g
//
LOCUS       DATMCV010000075         4309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_4611, whole genome shotgun sequence.
ACCESSION   DATMCV010000075 DATMCV010000000
VERSION     DATMCV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4611"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VNU03_01835"
     CDS             complement(<1..568)
                     /locus_tag="VNU03_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWP73215.1"
                     /translation="MTTMPVRYPDPAFQILDESFAKYRIFNAAVERLATGFRWAEGPV
                     WFGDGRYVLWSDIPNNRIMRWDEETGVVGVFRKPSNHSNGNTRDRQGRLITCEHDSRR
                     VTRTEYDGTVTVLMDRFENKRLNSPNDVVVKSDDSVWFTDPPFGILGNYEGHAAPVEL
                     PTNVYRFDPKTGQATVVAGDIPRPNGLCF"
     gene            731..1924
                     /locus_tag="VNU03_01840"
     CDS             731..1924
                     /locus_tag="VNU03_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HWP73216.1"
                     /translation="MPQAAAQIAIRPFQVNVPEAELTELRRRINATRWPEREPVTDTS
                     QGVRLATVQNLARYWATDYDWRKCEAKLTALPHFITEIDGLDIHFIHARSKHDDALPL
                     IVTHGWPGSVIEQLKIVDPLTDPTAHGASASDAFHLVIPSMPGYGFSGKPTTTGWGPE
                     RIARAWAELMRRLGYTKYVAQGGDWGALMVDMMGVQAPPELLGIHTNMAATVPPDVDK
                     AAFTGAPPPPTLSAEERQAFDQLAFFYKVDLAYALEMATRPQTLYGIADSPVGLAAWI
                     LDHDTRSLELIARVFDGRSEGLTRDDVLDNITHYWLTNTAVSSSRLYWENKLAFFAPK
                     GVAIPVAVSAFPDDICPAPRSWAERAYPKLIHYNKLDKGGHFAAWEQPQLFVAEVRAG
                     FKSLR"
     gene            1954..2592
                     /locus_tag="VNU03_01845"
     CDS             1954..2592
                     /locus_tag="VNU03_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWP73217.1"
                     /translation="MSKPAVRHLSKTDPTMRALIRRVGPCGLAIRNQQPFETLVRAVA
                     HQQIHGRAAEAILGRFIALFPGRGFPKPAAVAAMPASKMRRAGFSRGKIRAIKHIARK
                     ARDGRVPTRRACHALTDEILIERLTELHGVGQWTVEMLLIFTLGRPDVLPVDDFGVRE
                     GFKIAYGRRKQPTPKQLRRFGERWKPYRSVAAWYLWRAAEKALHRKIEPAVT"
     gene            complement(2615..3856)
                     /locus_tag="VNU03_01850"
     CDS             complement(2615..3856)
                     /locus_tag="VNU03_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWP73218.1"
                     /translation="MKIARVEALHCDGGWRPWTFVRVETDDGLVGWGECSDNRSPHGI
                     AGSVRDLTPLLIGQDPRPVERLYWDMLRATRQNLGGVTHKAMAGIELALWDIKAKALG
                     VPVYELFGGPMRDRMRLYWSHCGTTRARVGHVLGTPPLRTYDDITALGKEVVARGFTA
                     LKTNMVIPGDPATVYFPGFGSGINTTDGAPSVEILDAIERLIGTFRQAVGPKVGLCLD
                     LNYNFRTEGVLRIAKLLEQFDMQWVEYDNWDPQALLQIKQSTSTRLASCESLVTTRQY
                     RPFLELHAVDVAIIDVPWNGFSQAVQIGRMAETYEINIAPHNYYSHLADLHSLHLCAV
                     LPNVRIMEIDIDDVAWKGELVTKPPVVKNGHIFLPEGPGWGADINEEVLRAHPWPGSG
                     ANARSFYGMTPEQMAARPPAR"
     gene            complement(3900..>4309)
                     /locus_tag="VNU03_01855"
     CDS             complement(3900..>4309)
                     /locus_tag="VNU03_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP73219.1"
                     /translation="PYGSLEELRRLVAEPARAVLVTTKEGEFARLILDRWEVRLADVQ
                     GKETGVHKCLNLRGLIAAWVGAYGRRPRLAFVEDRLETLQHVTTHPDLDDVALFLAAW
                     GYNTEAARATAAADPRIRLLTLEQFRAGTAAWA"
BASE COUNT          674 a         1508 c         1444 g          683 t
ORIGIN      
        1 agaagcagag gccgttgggc cgcgggatgt cgcccgcgac cacggtggcc tggccggtct
       61 tcggatcgaa gcggtagacg ttggtgggca gctcgaccgg ggccgcgtgg ccctcgtagt
      121 tgccgaggat gccgaacggc ggatcggtga accacacgct gtcgtcggac ttcaccacca
      181 cgtcgttggg cgagttgagc cgcttgttct cgaagcggtc catcaggacg gtgaccgtcc
      241 cgtcgtattc ggtgcgggtc acccgccggc tatcgtgctc gcaggtgatg agccggccct
      301 ggcggtcgcg ggtgttgccg ttggagtggt tcgacggctt gcggaagacg ccgacgactc
      361 ccgtctcctc gtcccaccgc atgatccggt tgttggggat gtcgctccac agcacgtagc
      421 gtccgtcccc gaaccacacc gggccctcgg cccagcggaa cccggtggcg agccgctcca
      481 ccgccgcgtt gaagatgcgg tacttggcga agctctcgtc gagaatctgg aacgcgggat
      541 cgggatagcg cactggcatg gtcgtcatgg cttccggatg gtcgccacta ccgcgggaga
      601 agtcaacccg cattttcgtc tcgaagctct tgacggggcc cttctttcgc gagattctcc
      661 gcatgcaccg aagtcctcga cgcagcgcac gcaacaccct cgtggctcac gcaaccgcaa
      721 ggagcacacc atgcctcagg ccgccgccca gattgcgatt cgtccgttcc aggtaaatgt
      781 tccggaagcg gagctcaccg agttgcgccg gcgcatcaac gcgacaaggt ggcccgagcg
      841 ggaaccggtc acggatacat cgcaaggcgt gcggctcgcc acggttcaga atctcgcgcg
      901 ctactgggcg accgactacg actggcgcaa gtgcgaggcg aagctgaccg ccctaccgca
      961 cttcatcacc gagatcgatg ggctcgacat tcacttcatc cacgctcgtt cgaagcacga
     1021 tgatgcgctg ccgctcatcg tcacgcacgg ctggcccggc tcggtcatcg agcagctgaa
     1081 gatcgtcgat ccgctgaccg atcccaccgc ccacggcgcg agcgcgtcgg acgccttcca
     1141 cctggtgatc ccgtcgatgc cgggctacgg attctccggc aagccgacca ccaccggctg
     1201 gggccccgag cgcatcgcac gagcctgggc ggagctgatg aggcgcctcg gctacacgaa
     1261 gtacgtggct caaggcggcg actggggcgc gctcatggtc gacatgatgg gggtacaggc
     1321 gcccccggaa ctgctcggaa tccacaccaa catggccgcc acggttccgc ccgacgtcga
     1381 caaggcggcc ttcaccgggg ctccgccgcc gcccaccctg tcagccgagg aacggcaggc
     1441 gtttgaccag ctcgccttct tctacaaggt cgacctggcc tacgcgctgg aaatggcgac
     1501 ccgcccccag acgctgtacg ggatcgcgga ttccccggtc gggctggccg catggatcct
     1561 cgaccatgac acccgcagcc tcgagctcat cgcgcgggtc ttcgacggtc ggtccgaggg
     1621 cctcacgcgg gacgacgtgc tcgacaacat cacgcactac tggctgacga atacggcggt
     1681 ctcctcctct cgtctgtact gggagaacaa gctggccttc ttcgccccga aaggcgtcgc
     1741 catccccgtc gcggtgagcg cgttcccgga tgacatctgc ccagctccgc ggagctgggc
     1801 ggagcgcgcg taccccaagc tcatccacta caacaagctc gacaagggcg ggcacttcgc
     1861 ggcctgggag cagccgcagc tcttcgtggc cgaggtgcgc gcggggttca aatcgttgcg
     1921 gtagtcgcga ctccgcgcta gcatcacgcg tcgatgagca agcccgcggt gcggcatctc
     1981 tccaagacgg acccgacgat gcgcgcgctc atccgccggg tcgggccctg cggtctcgcg
     2041 atccgcaacc agcagccgtt cgagaccctg gtccgcgccg tcgcgcacca gcagatccac
     2101 ggtcgcgccg cggaggcgat cctgggccgc ttcatcgcgc tgttcccggg ccggggattc
     2161 cccaagccgg ccgcggtcgc ggcgatgccc gcgtcgaaga tgcggcgcgc ggggttctcg
     2221 cgggggaaga tccgcgccat caagcacatc gcccgcaagg ctcgcgacgg ccgcgtgccc
     2281 acccggcgcg cctgtcacgc gctgaccgac gagatcctga tcgagcgtct caccgagctg
     2341 cacggagtcg gccagtggac ggtggagatg ctgctgatct tcacgctggg ccggcccgac
     2401 gtcctccccg tggacgactt cggggtccgc gagggcttca agatcgccta cggccggcgc
     2461 aagcagccga ccccgaagca gctccggcgc ttcggtgagc gctggaagcc ctaccgctcg
     2521 gtcgcggcct ggtacctctg gcgcgcggcc gagaaggcgc tgcaccgcaa gatcgagccc
     2581 gcggtcacgt agcgaacgat cacgtagcga gcgatcaccg ggccggtgga cgcgccgcca
     2641 tctgctcggg cgtcatgccg tagaaactcc gcgcgttcgc ccccgatccg ggccacgggt
     2701 gcgcgcgcag cacctcctcg ttgatgtcgg cgccccagcc cggtccctcc ggcaggaaga
     2761 tgtggccgtt cttgaccacc gggggcttcg tcaccagctc ccccttccag gccacgtcgt
     2821 cgatgtcgat ctccatgatg cgcacgttcg gcagcaccgc gcagaggtgg agcgagtgga
     2881 gatcggccag gtggctgtag tagttgtggg gcgcgatgtt gatctcgtac gtctcggcca
     2941 tgcgcccgat ctggaccgcc tgcgagaacc cgttccacgg cacgtcgatg atggccacgt
     3001 cgaccgcgtg cagctccagg aacggccgat attgacgagt cgtcaccagg ctctcgcagg
     3061 aggccagccg cgtcgaggtc gactgcttga tctgcaggag ggcctgcgga tcccagttgt
     3121 cgtactcgac ccactgcatg tcgaactgct cgagcagctt ggcgatccgc agcacgccct
     3181 cggtgcggaa gttgtagttg agatcgaggc agaggccgac cttcgggccc accgcctgtc
     3241 ggaaggtgcc gatgagccgc tcgatcgcgt ccaggatctc cacgctgggc gcgccgtcgg
     3301 tggtgttgat gccgctgccg aagcccggga aatagacggt agccggatcg cccgggatca
     3361 ccatgttggt cttgagcgcg gtgaacccgc gcgccaccac ttccttgccg agcgcggtga
     3421 tgtcgtcgta ggtgcggagc ggcggcgtgc ccagcacgtg gcccacgcgc gctctggtcg
     3481 tcccgcagtg cgaccagtag agccgcatgc ggtcgcgcat cgggccgccg aagagctcgt
     3541 agaccggcac ccctagcgcc ttggccttga tgtcccacag cgccagctcg atgccggcca
     3601 tcgccttgtg ggtcaccccg cccaggttct ggcgggtggc gcggagcatg tcccagtaga
     3661 gccgctcgac cggccgggga tcctggccga tgagcagcgg ggtcaggtcg cgcaccgagc
     3721 ccgcgatgcc gtgcgggctc cggttgtcgc tgcattcgcc ccagcccacc aaaccgtcgt
     3781 cggtctcgac gcggacgaag gtccagggcc gccagccgcc gtcacagtgg agcgcctcga
     3841 cgcgtgcgat cttcatggcg agagacgatg ccacacccgg cgggcccgga caagcctccc
     3901 tacgcccacg cggcggtgcc ggcgcggaac tgctcgagcg tgagtagccg gatccgcgga
     3961 tcggccgccg cggtggcccg cgcggcctcc gtgttgtagc cccacgcggc caggaagagc
     4021 gccacgtcgt cgaggtcggg atgcgtcgtg acgtgctgga gcgtctcgag ccggtcctcg
     4081 acgaaggcca ggcgcggccg ccgcccgtag gccccgaccc acgccgcgat gaggccgcgc
     4141 aggttcaggc acttgtgcac gccggtctcc ttgccctgca cgtcggccag gcgcacctcc
     4201 cagcggtcga ggatgagccg cgcgaactcg ccctccttgg tggtgacgag caccgcccgg
     4261 gccggctcgg cgacgaggcg gcgcagctcc tcgagcgagc catagggcg
//
LOCUS       DATMCV010000076         4619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_137989, whole genome shotgun sequence.
ACCESSION   DATMCV010000076 DATMCV010000000
VERSION     DATMCV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137989"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VNU03_01860"
     CDS             complement(<1..203)
                     /locus_tag="VNU03_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWP73220.1"
                     /translation="MMKAVVLHEADEMRVEQRPVPDPASGEVLLKVNVTSICGTDVKV
                     LHRKLLGQPDGPFIMGHEYAGTVA"
     gene            324..1496
                     /locus_tag="VNU03_01865"
     CDS             324..1496
                     /locus_tag="VNU03_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWP73221.1"
                     /translation="MRDLLDRLDGWRDEQLELLARLVNHDSGTDDVLDVNRVGAILAE
                     RLERLGFSLRRVATERFGDHLVGEKRGAGPKRLLFVGHYDTVFSSGTAKQRPFRIDGQ
                     GRAWGPGVYDMKGGLAALLYALRAHQEARTSAWAETTVAVVFNSDEERLSPTSRPVIE
                     AEARRAHSVGILEPARPGGEYVMARKGAGTFHLEIQGKASHAGLQPELGASAIWDLAQ
                     KVAALHGLTDLGTGVTVNVGTVRGGERPNVVAAHAAAEIDLRAWSQADADATIAAMRT
                     ICERSHVAGTRARFQGQIHFPPWPPGLPGTERLLEIMRGAGRELGVEVRAIKTGGGSD
                     GNHTSAIAPTLDGMGPKGSRAHSDEEFIEVATLLERTKMIALFLDRWASEFEIPAR"
     gene            complement(1507..2475)
                     /locus_tag="VNU03_01870"
     CDS             complement(1507..2475)
                     /locus_tag="VNU03_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWP73222.1"
                     /translation="MSGPAAPLLELVDLARHFPVRSAFGRRVGWLRAVDGVSLRVRPG
                     ETLGLVGESGCGKSTLGKTVMGIYPPTAGEIRFAGREIGRLPPRARRAVAKDLQYVYQ
                     DPGASLDPRWKIGPSLHEPLRIHTTLTRTERDREVRAILAAVGLPDAHLDLYPHELSG
                     GQQRRVGLARILTLRPRLVILDEPTSGLDVSVQASVLKLFRSLQETFALTYVFISHDL
                     AVVRAMCAGIAVMYLGKIVECGPTNRVFEAPRHPYTQSLLAAVPRIGGRRVTQDFALE
                     GEPPNPRDVPSGCRFRTRCPLAQARCAEEEPALRPLDGRTVACHFA"
     gene            complement(2472..3506)
                     /locus_tag="VNU03_01875"
     CDS             complement(2472..3506)
                     /locus_tag="VNU03_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73223.1"
                     /translation="MAELLAVRGLQVDFATDRGPAQVLDRINLDIATGEVVGLVGESG
                     CGKTTLARAILGILPEGAARIRGGEVRFQGTDLLRQDPASVNERVRGRAITFIPQDPF
                     TSFSPLFPIGTQIMDLMKWKSPRARDGAGPSSIFGRYPRERYRTDREAVLDTLRAVQI
                     PEPARALRRLPHEFSGGQRQRLMIAMALLPQPDLIIADEPTTALDVTIQAQILRLLRA
                     LVKERGVSVLFTTHDLGTAHEICDRVVVMYAGQEMEAAPTDAFFGRPAHPYTRRLLAS
                     VPGPDGEIREIRGEVPSLVTPPSGCRFHPRCDYATAECRDQRPAPRALAADHGVRCHH
                     PVDAPAGAGA"
     gene            complement(3499..4362)
                     /locus_tag="VNU03_01880"
     CDS             complement(3499..4362)
                     /locus_tag="VNU03_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73224.1"
                     /translation="MTAAWFVVTRLARDRSALIGLVLIALLVLAALLAPVLATHPDDV
                     FELHPSERLKEPSGAHWLGTDRMGADVYSRLLFGARITILIGVIAVGASLAIGVPIGL
                     VAGYHHGWASDLLMRVSDIFLAVPQVVLAIAIAQTLGPSLPNVILALSVTYWPWFARV
                     VYAETRALQKEVFVESTTALGASPWRLTVLHVLPNIASPVIVRASIGMGFTILTAAAL
                     GFLGLGPPPPTPEWGRTIAEAREFLPDAWWYAAAPGVAIFLTVMGFNLLGDGLRDVLD
                     PRLRRSAARDG"
     gene            complement(4359..>4619)
                     /locus_tag="VNU03_01885"
     CDS             complement(4359..>4619)
                     /locus_tag="VNU03_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73225.1"
                     /translation="KYVLRSALTSTVTQIGLLFGILLAGAVVTEAVFDWPGIGTYAVN
                     SILRSDYNAVMGFTVWAGAIFIVVNLLVDLAHTLVDPRERTS"
BASE COUNT          691 a         1616 c         1730 g          582 t
ORIGIN      
        1 gccaccgtcc ccgcgtactc gtgccccatg atgaagggcc cgtcgggctg gccgaggagc
       61 ttgcggtgca ggaccttgac gtcggttccg cagatggagg tgacgttgac cttgagcagg
      121 acctcccccg atgccggatc gggcaccggc cgctgctcca cgcgcatctc gtcggcctcg
      181 tgcaggacca ccgctttcat catcgccgcg ccacctcccg cgcgacggga gtctacgtgg
      241 acggagagac cgctgtcaaa ggcctatcac gccgatctgc acgtgcgttc cctccgaggg
      301 cgctccacgc taccatgtcg gccatgcggg atctcctgga ccggctcgac ggctggcgcg
      361 acgagcagct agagctcctg gcccgcctcg tcaaccatga cagcggcacc gacgacgtgc
      421 tggacgtcaa ccgcgtcggg gccattctcg ccgagcggct ggagcgcctg ggtttctcgc
      481 tccgccgggt ggccaccgag cgcttcggcg accacctcgt cggagagaag cggggagcgg
      541 gccccaagcg cttgctcttc gtcggccact acgacaccgt gttctcctcc gggaccgcca
      601 agcagcggcc gttccggatc gacgggcagg ggcgcgcgtg gggacccggc gtgtacgaca
      661 tgaagggcgg gctcgccgcg ctgctgtacg ctctgcgcgc gcaccaggag gcgcgcacct
      721 ccgcctgggc cgagacaacg gtcgcggtgg tcttcaactc ggacgaagag cggctctcgc
      781 ccacctcccg gccggtgatc gaggccgagg cgcggcgggc gcacagcgtg ggcatcctcg
      841 agccggcgcg cccgggcggc gagtatgtca tggcgcgcaa aggagccggc acgtttcacc
      901 tggagatcca gggcaaggcc tcccacgccg ggctccagcc cgagctgggc gcgagcgcca
      961 tctgggatct ggcgcagaag gtggcggcgc tccacgggct gaccgacctc ggcaccggcg
     1021 tcaccgtgaa cgtggggacg gtgcgcggtg gggagcgccc gaacgtcgtg gcggcccacg
     1081 ccgcggccga gatcgatctg cgcgcgtgga gccaggcgga cgcggacgcg acgatcgccg
     1141 ccatgcggac gatctgcgag cggtcgcacg tcgcgggcac ccgcgcccgc ttccaggggc
     1201 agatccactt cccgccgtgg ccgcccgggc tgccgggcac ggagcggctg ctggagatca
     1261 tgcgcggggc aggccgcgag ctcggcgtcg aggtgcgggc catcaagacg ggcggcggct
     1321 cggacggcaa tcacacctcg gccatcgcgc ccaccctcga cggcatgggg ccgaagggca
     1381 gccgggcgca cagcgacgag gagttcatcg aggtggccac gctgctcgag cggacgaaga
     1441 tgatcgccct cttcctggat cgctgggcct cggagttcga gatcccggcg cgctgagggc
     1501 cgctcgtcag gcgaagtgac aggccacggt gcgcccgtcg agcggacgga gcgccggctc
     1561 ctcctccgcg caccgggcct gggccagcgg gcaccgagtc cggaagcggc agccgctcgg
     1621 cacgtcgcgc gggttcggcg gctcgccctc gagcgcgaag tcctgggtga cccggcggcc
     1681 gccgatgcgg ggcaccgcgg ccagcagcga ctgcgtgtaa ggatgacgcg gcgcctcgaa
     1741 cacgcggttg gtcgggccgc attcaacaat tttgccgagg tacatcaccg cgatgcccgc
     1801 gcacatcgcg cgcaccaccg cgagatcgtg cgagatgaag acgtaggtga gcgcgaacgt
     1861 ttcctggaga ctccggaaca gcttgagcac gctggcctgg accgagacgt cgagtccgga
     1921 ggtcggctcg tccaggatca ccaggcgggg tcgcagggtc aggatgcgcg cgagccccac
     1981 ccggcgctgc tggccgccgc tcagctcgtg cgggtagagg tcgaggtgcg cgtccgggag
     2041 cccgaccgcg gccaggatcg cgcgcacctc ccggtcccgc tcggtccggg tcagcgtggt
     2101 gtggatccgg agcggctcgt gcagcgaggg cccgatcttc caccggggat cgagcgaggc
     2161 gcccgggtcc tggtagacat actgcagatc cttggcgacc gcgcggcgcg cccggggggg
     2221 caatcgcccg atctcgcggc cggcgaagcg gatctcaccc gcggtcggcg ggtagatgcc
     2281 catcaccgtc ttgccgagcg tcgacttgcc gcagccgctc tcgccgacga ggccgagcgt
     2341 ctcgcccgga cgcacccgaa gcgagacgcc gtcgacggcc cggagccaac cgacgcggcg
     2401 gccgaaggcg ctgcgcaccg ggaagtggcg cgcgaggtcg accagctcca gcagcggcgc
     2461 ggcggggccg ctcacgcgcc ggctccggcg ggggcgtcga ccggatggtg gcagcggacg
     2521 ccgtggtcgg cggcgagcgc gcgcggcgcc ggacgctggt cgcggcactc cgcggtcgcg
     2581 tagtcgcagc gcgggtggaa gcggcacccc gacggcgggg tgaccaggct cgggacctcg
     2641 ccccggatct cgcggatctc gccgtcgggg cccggaacgc tggcgagcag gcgcctcgtg
     2701 taggggtggg cggggcggcc gaagaaggcg tccgtcggcg ccgcctccat ctcctggccg
     2761 gcgtacatga ccaccacgcg gtcgcagatc tcgtgcgcgg tcccgagatc gtgggtggtg
     2821 aagagcaccg acaccccgcg ctccttcacc agcgcgcgca ggagccggag gatctgggcc
     2881 tggatcgtca cgtcgagcgc ggtggtgggc tcgtccgcga tgatcagatc gggctgcggg
     2941 agcagggcca tcgcgatcat gaggcgctgt cgctggccgc ccgagaactc gtggggcagc
     3001 cggcgcaggg cccgcgccgg ctcggggatc tgcaccgcgc gcagggtgtc cagcaccgcc
     3061 tcgcggtcgg tgcgatagcg ctcccgcgga tagcgcccga agatggacga cggcccggcc
     3121 ccgtcgcgcg cgcgcgggga cttccacttc atcaggtcca tgatctgggt gccgatgggg
     3181 aacagcgggc tgaacgaggt gaacggatcc tgcgggatga acgtgatcgc gcggccgcgg
     3241 acgcgctcgt tcacggacgc ggggtcctgc cgcagcaggt cggtgccctg gaagcgcacc
     3301 tcgccgccgc ggatccgcgc ggccccctcg ggcaggatgc cgaggatcgc gcgggcgagg
     3361 gtggtcttgc cgcagccgct ctcgccgacg agccccacca cctccccggt cgcgatgtcc
     3421 aggttgatcc ggtcgagcac ctgcgccggc ccgcggtcgg tggcgaagtc cacctgcagc
     3481 ccgcggacgg cgagtagctc agccatcgcg agccgcgctc cggcgcagcc gcggatcgag
     3541 cacgtcgcgc aggccgtcgc cgagcaggtt gaagcccatg acggtgagga agatggccac
     3601 ccccggcgcg gccgcgtacc accacgcgtc gggcaggaac tcgcgcgcct ccgcgatcgt
     3661 ccgtccccac tccggggtgg gcggcggggg cccgagcccg aggaagccga gcgcggcagc
     3721 ggtcaggatc gtgaacccca tgccgatcga cgcccgcacg atgacgggcg aggcgatgtt
     3781 cggcagcacg tgcagcacgg tgagccgcca cggcgaggcg ccgagggcgg tcgtgctctc
     3841 gacgaagacc tccttctgca gcgcccgcgt ctcggcgtag acgacgcgcg cgaaccaggg
     3901 ccagtaggtg accgagagcg ccaggatgac gttggggagc gacgggccca gcgtctgggc
     3961 gatggcgatg gcgagcacca cctgcggcac ggcgagaaag atgtcggaga cccgcatgag
     4021 cagatcgctc gcccagccgt ggtggtagcc ggcgacgagc ccgatcggga ccccgatggc
     4081 cagggaggcg cccaccgcga tgacgccgat gaggatggtg atccgcgccc cgaagaggag
     4141 ccggctgtag acgtcggcgc ccatccggtc ggtgccgagc cagtgcgcgc cgctcggctc
     4201 cttgaggcgc tcggaggggt gcagctcgaa tacgtcgtcc gggtgcgtcg ccagcaccgg
     4261 cgcgagcagg gcggccagca ccagcagcgc gatgagcacc aggccgatca gcgccgaccg
     4321 gtcgcgcgcc agcctcgtca ccacgaacca cgccgcggtc atgacgtgcg ctcccgcgga
     4381 tcgacgaggg tatgggcaag gtcgacgagc aggttcacga cgatgaagat cgccccggcc
     4441 cagaccgtga agcccatcac cgcgttgtag tccgagcgca ggatggagtt gaccgcgtag
     4501 gtgccgatgc ccggccagtc gaacaccgcc tccgtcacca ccgcccccgc gagcaggatc
     4561 ccgaagagca ggccgatctg cgtcaccgtg gaggtgagcg ccgagcggag gacgtactt
//
LOCUS       DATMCV010000077         3400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_469426, whole genome shotgun sequence.
ACCESSION   DATMCV010000077 DATMCV010000000
VERSION     DATMCV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469426"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VNU03_01890"
     CDS             <1..491
                     /locus_tag="VNU03_01890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP73226.1"
                     /translation="PDTTLLIAGGGPLLGELRAQVSRVGLGDRVVFTGYVAEAEKADH
                     FNLGDVFLFPSAMEGFGLVVAEAMSSGLPVVASNRGSIPELVVDGEGGFVCDPAGPEP
                     FVEKLALLLGDRALGEKFGHANRERVERMFRWERCVDGTRRVYEEAVERWHRRGPEGR
                     TR"
     gene            488..1276
                     /locus_tag="VNU03_01895"
     CDS             488..1276
                     /locus_tag="VNU03_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP73227.1"
                     /translation="MNGLQRLLVRGVDLVNRRGKSVGVRLVKLTGKRPYAIHPKHLVE
                     QPWHDWYLPYLAGSDFVLDVGCSNGAHLVRAATACRSIVGFDYDLPQLAIAGRTIREQ
                     RLARARVLAWDITRPFPFPDRTFEKALFLDVIEHLHPRVAVLREIHRVLKDEGRLLVS
                     GPNRENSWRRTLRAAGLFAYSDPDHKIEYTRDEFLDELTQAGFEPEGAVMPVVLDTPW
                     AGVIDAVGGVSLGLYDRLSRWKRDAALRRPEESTGFRVVARKRR"
     gene            1276..2385
                     /locus_tag="VNU03_01900"
     CDS             1276..2385
                     /locus_tag="VNU03_01900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP73228.1"
                     /translation="MRVGLLPALGGGIRALAQTGQQSRLIDGYFRPYARAFDGVDYFS
                     YLPESLAEFTDDATLRERVRLHAPPGPVARGRRAVQIPFAHAAEMRRCAALRVFQITG
                     VIPALIARRRFGIPYVTTYGFWYAALSQRGPKRVLKGFVERLGLKYASAVIATTPDLA
                     ERAKRLAPRVELIPNGVDTGLFRPPARQPENKSSRILYVGRLSAEKNLGAIVTAVSHV
                     GERAMVVMVGSGPLQGALEAQARQERVAVEFPGVVDQRRLPEVYGAADVFVLASFTEG
                     HPKVLLEAMACGVPCVASDCAGNRSLVTDDRTGLLFDPHQPKQLADRLERLMTEPGLG
                     RRLADAARAEIVARYDLRALVEREIALVRQVAGGG"
     gene            2458..3204
                     /locus_tag="VNU03_01905"
     CDS             2458..3204
                     /locus_tag="VNU03_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP73229.1"
                     /translation="MDELLRIARESPLTELIERHLPAGGRLLEAGCGLGQYVLLFRER
                     GRAAVGADWSQEALRAGAGLGAPLAAMDLRALAVVTGGIAAYLSLGVVEHDPDGPDAI
                     LREAARVVPSGGALLLSVPYWNGVRRLAAPYRRRQARHTREAGGQFYQYAFTRRELGR
                     RLAAHGFSVASFHPYDPGRVLRKALRRLRGGSRPPAAAPAPGPGARVVAASTRPAGRS
                     PLRAAARALLYSAPSLRLFGHMLLAVAVRR"
BASE COUNT          416 a         1219 c         1258 g          507 t
ORIGIN      
        1 atccggacac cacgctgctg atcgcgggcg ggggaccgct cctgggggag ctgcgcgcgc
       61 aggtgagccg cgtgggcctg ggcgatcggg tggtcttcac cggctacgtg gcggaggcgg
      121 agaaggcgga ccacttcaac ctgggcgacg tgttcctgtt cccgtccgcg atggaaggct
      181 tcgggctcgt ggtggcggag gcgatgtcct ccgggctccc ggtggtggcc tcgaaccgcg
      241 gctccatccc ggagctggtg gtggacggcg agggcggatt cgtctgcgac ccggccggac
      301 ccgagccgtt cgtggagaag ctggccctcc tgctcggcga tcgcgccctc ggggagaagt
      361 tcggccacgc caaccgcgag cgcgtcgagc ggatgtttcg ctgggagcgg tgcgtggacg
      421 gcacccggcg cgtctacgag gaggcggtcg agcggtggca ccggcgcggg ccggaaggcc
      481 ggacgcgatg aacgggctgc agcgcctgct cgtgcgcggg gtggacctgg tgaaccgccg
      541 tggcaagtcg gtgggcgtgc gcctggtcaa gctgaccggc aagcggccgt acgcgatcca
      601 tccgaagcac ctggtcgagc agccgtggca cgactggtac ctgccgtatc tcgcgggaag
      661 cgacttcgtg ctcgacgtgg gctgcagcaa cggcgcgcac ctggtccgcg cggccaccgc
      721 gtgccgctcg atcgtgggct tcgactacga cctcccccag ctcgccatcg cgggaaggac
      781 catccgggag cagcggctcg cacgggcccg ggtgctggcc tgggacatca cgcgtccctt
      841 cccgttcccg gaccggacct tcgagaaggc cctcttcctc gacgtcatcg agcacctcca
      901 cccgcgggtg gcggtgctgc gcgagatcca ccgcgtgctg aaggacgagg gccgcctgct
      961 cgtctcggga ccgaaccggg agaactcgtg gcgccggacg ctgcgcgcgg ccgggctctt
     1021 cgcctactcc gatcccgacc acaagatcga gtacacccgc gacgagttcc tcgacgagct
     1081 gacccaggcc ggcttcgagc cggaaggcgc ggtgatgccg gtggtgctcg atactccgtg
     1141 ggcgggggtg atcgacgcgg tgggcggcgt gtcgctcggg ctctacgatc gcctgagccg
     1201 ctggaagcgg gacgcggccc tccggcggcc ggaggagagc accggcttcc gcgtcgtggc
     1261 ccggaagcgc cgctgatgcg ggtgggcctg ctcccagctc tgggcggcgg catccgggcg
     1321 ctggcccaga ccgggcagca gtcgcgcctg atcgacggct acttccgccc ttacgcccgc
     1381 gccttcgacg gcgtcgacta tttcagctac ctgcccgagt cgctcgccga gttcaccgac
     1441 gacgccacgc tgcgcgagcg cgtgcgcctg cacgctccgc ccggcccggt ggcgcgcggt
     1501 cgacgcgcgg tgcagatccc gttcgcgcac gcggccgaga tgcggcggtg tgccgccctg
     1561 cgcgtgttcc agatcaccgg ggtcatcccg gccctgatcg cgcggcggcg cttcggcatc
     1621 ccgtacgtga ccacctacgg cttctggtac gccgcgctct cccagcgcgg ccccaagcgc
     1681 gtgctcaagg gattcgtcga gcggctcggc ctcaagtatg cgtcggcggt gatcgcgacc
     1741 acgcccgacc tggccgagcg ggcgaagcgg ctggcgccgc gggtggagct gatcccgaac
     1801 ggagtggaca ccgggctctt ccggccgccg gcccggcaac cggagaacaa gtcgagccgc
     1861 atcctgtacg tggggagact ctccgccgag aagaacctcg gcgcgatcgt caccgcggtc
     1921 agccacgtgg gtgagcgagc gatggtggtg atggtgggct ccgggccgct acagggcgcg
     1981 ctcgaagcgc aggcgagaca ggagcgtgtc gcggtggagt ttcccggcgt ggtggaccag
     2041 cgccgcctgc ccgaggtcta cggcgcggca gacgtctttg tcctcgcctc cttcacggaa
     2101 ggccatccga aggtgctgct cgaggcgatg gcctgcgggg tcccgtgtgt ggcctccgac
     2161 tgcgcgggca accgatccct cgtgaccgac gaccgcaccg ggctgctctt cgatcctcac
     2221 cagccgaagc agctggccga tcgcctcgag cgtctgatga cggagcccgg tctcggccgg
     2281 cgccttgcgg acgccgcgcg cgccgagatc gtcgcccgct acgacctgcg cgcgctcgtg
     2341 gagagggaga tcgccctcgt gcggcaggtc gcgggcggag gctgagccat ccccctcgcg
     2401 tactactcgc gctcggccac caccgagttc tggaccgagc actggggcgg ccacacggtg
     2461 gacgagctgc tccggatcgc gcgcgagtcg cccctcaccg agctgatcga gcgccacctg
     2521 cccgcggggg ggcgcctgct ggaggccggc tgtggactcg gccagtacgt cctgctgttc
     2581 cgcgagcgcg ggcgtgccgc ggtcggcgcc gactggagcc aggaggcatt gcgggcgggg
     2641 gcgggcctcg gggcgccgct cgcggccatg gaccttcgcg cgctcgcggt ggtcaccggc
     2701 gggatcgccg cctatctgtc actcggcgtc gtggagcacg atcccgacgg ccccgacgcg
     2761 atcctgcgcg aggccgcccg tgtcgtgcct tccgggggtg cgctgctcct ctcggtgccc
     2821 tactggaacg gcgtgcgccg gctggccgcg ccctaccggc gccggcaggc gcgtcacacc
     2881 cgcgaggccg gcgggcagtt ctatcaatac gccttcaccc gccgcgagct cggccgccgc
     2941 ctcgccgccc acgggttctc ggtcgcgagc tttcatccct acgatccggg gcgggtgctc
     3001 cgcaaggcgc tgcgtcggct ccgcggcgga tctcggccgc cggcggccgc gccggcaccc
     3061 ggcccgggcg cccgcgtggt cgccgcgtcg acgcgtccgg ctggccgctc cccccttcgc
     3121 gcggcggcgc gcgcgctgct ctactcggcg ccctcgctac gcctcttcgg ccacatgctg
     3181 ctggcggtgg cggtccggcg ctagccgcgt tgctatactg ctcgcgatga gcgagccctc
     3241 gttcgagctg gacgatctgg gggaccggcg ggtgctcacg gtggacggcc gacgccacga
     3301 gacgcgctac agcgcgcgcg tggtccggct gctcatcgag cgcaagggcg cgcaccgggc
     3361 gccgctctac tttcccttca aggagcagcg tgcccgcctc
//
LOCUS       DATMCV010000078         3679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_469446, whole genome shotgun sequence.
ACCESSION   DATMCV010000078 DATMCV010000000
VERSION     DATMCV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469446"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VNU03_01910"
     CDS             complement(<1..749)
                     /locus_tag="VNU03_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWP73230.1"
                     /translation="MRIALDRGTGRAGKAPLSRQIQLHFERLISQGLLGSGVKLPATR
                     ELAQELGVNRTTVALAYDELVAGGWARAHVGQGTFVADRVPAPVGMPRAAAPRLDWTG
                     FLSKGAQVIAADTRRRRGYSQVPRTGPGAISFAGGMPDSGLFPTEAFRRVLNRVIREE
                     GPELLQYYPARGYPPLREFLSGYLLRFGLEARPEEILIVNGSQQGFDLVARTLLDPGD
                     FVAIEEPSYPRAMQVFRSFGAQLLPVPMVNGG"
     gene            921..1655
                     /locus_tag="VNU03_01915"
     CDS             921..1655
                     /locus_tag="VNU03_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73231.1"
                     /translation="MPGVVVLESMWNKKGRLLDDEPSVLPYLKAIRQSLAWEGIRVNL
                     VYRRFYSSYDLGLLLEEVRRRRRSFQVCYIASHGQRRKLVGIGDKVIRLETLVDHCRP
                     STGTGYIFGACDFVTPDTARNFLLSTGARFVAGYQKAIPWTESMLVDCLFLSYLLGGT
                     MKWVRTKHGERMPPLRALEDFEITRSENPQKVAGQLYRDFPLAHDITFSCFTLDRAQG
                     RKPTMDNSFDIFKRNVDRARRVVRDL"
     gene            1678..1854
                     /locus_tag="VNU03_01920"
     CDS             1678..1854
                     /locus_tag="VNU03_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73232.1"
                     /translation="MSAGEAPIKQAVKWIDDQLHDDPKADRTRLIDEAGRRFDLTPLD
                     SEFLLRHLSQRHPS"
     gene            1871..2650
                     /locus_tag="VNU03_01925"
     CDS             1871..2650
                     /locus_tag="VNU03_01925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP73233.1"
                     /translation="MSESQQARVQAQFGPSAAAYVKSPGHAAGPDLEQLIAWGKKRGA
                     ARVLDIATGGGHTALAFSRFTPSVVAVDVTLPMVQAAREFATAEGATGVRFLGADVEA
                     LPFRDGAFGTVTCRTAAHHFPALLPALRQVARVLRAGGSFLVEDIQGHDDEEASAFIL
                     EVERRRDPSHVRAAFRHLEWTAFLRAAGLTVIDEGGMSKVRAWDDWTRRSRMTSAAKA
                     ELEAYVLAAPARCREAFDFHIDGGRIVSFTDRMLLLRADRD"
     gene            complement(2647..2928)
                     /locus_tag="VNU03_01930"
     CDS             complement(2647..2928)
                     /locus_tag="VNU03_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HWP73234.1"
                     /translation="MDEPGPDPIILPIEDALDLHPFAPRDVRSVVDEYLRQAAARGFR
                     EVRLIHGKGTGTQRAIVRSVLAGHPAVERFFDGPPDRGGWGATVVVLKA"
     gene            3029..3442
                     /locus_tag="VNU03_01935"
     CDS             3029..3442
                     /locus_tag="VNU03_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWP73235.1"
                     /translation="MSDARSEALDWLSTFEAACRGRDFEAGRRLFAPDAVAFGTYATA
                     VHGLDNIEREQWRQIWPRIRGFRFDAEPAVAGTGDSAWIAATWASEATGPDGQPFTRH
                     GRATFNLARRDGRWLAVHSHVSLLPSQSESAHGRR"
BASE COUNT          538 a         1395 c         1226 g          520 t
ORIGIN      
        1 ccgccgttca ccatgggcac cggcaggagc tgggccccga acgatcggaa gacctgcatg
       61 gcgcgtgggt acgacggctc ctcgatcgcc acgaagtccc ccggatcgag gagcgtgcgc
      121 gccaccaggt cgaagccctg ctgcgagccg ttgacgatca ggatctcctc cgggcgtgcc
      181 tcgaggccga agcgcaggag atagccggag aggaactccc gcagcggcgg gtacccgcgg
      241 gccgggtaat actgcagcag ctcgggcccc tcctcgcgga tcacccggtt gagcacgcgc
      301 cggaaggcct cggtcgggaa gagcccgctg tccggcatgc ctcccgcgaa cgagatggcg
      361 cccgggccgg ttcgcggcac ctggctgtag ccccgccgcc ggcgggtgtc ggccgcgatc
      421 acctgggccc ccttcgagag gaagccggtc cagtcgagcc gcggcgccgc cgcgcggggc
      481 atcccgaccg gcgccggcac gcggtccgcg acgaaggtcc cctggccgac gtgggctcgc
      541 gcccagcccc ccgccaccag ctcgtcgtag gccagggcca ccgtcgtgcg gttgaccccg
      601 agctcctggg cgagctcccg ggtggccggc agcttcaccc ccgagccgag caggccctgg
      661 ctgatcagcc gctcgaaatg cagctggatc tgccgcgaga gcggggcctt ccccgcccgc
      721 ccggtccccc tgtcgagtgc gatcctcata ccttgctatg gctccctgat acccgccatt
      781 atcgaaagaa tcaagtgact gtaaacctcc aattggatga ttccaaacca gtccaatata
      841 cccggtccgg accccgtggt atcatggcgc gcccagggct ccctgggctt cccaggcgac
      901 cgctccgagg ggaccgaacg atgccgggtg tggtggtgct cgagtcgatg tggaacaaga
      961 agggccgtct gctggacgac gagccctcgg tcctccccta cctcaaggcc atccgccagt
     1021 cgctggcctg ggagggcatc cgggtcaacc tggtctaccg ccgcttctac tcgagctatg
     1081 acctgggcct gctcctcgag gaggtccggc gccggcgccg ctccttccag gtctgctaca
     1141 tcgcctcgca cggccagcgt cgcaagctgg tcggcatcgg cgacaaggtg atccggctgg
     1201 agaccctggt cgaccactgc cgtccgagca cgggcaccgg ctacatcttc ggggcgtgcg
     1261 acttcgtcac tcccgacacc gcgcgcaact tcctgctctc gaccggtgcg cgattcgtgg
     1321 ccggctacca gaaggcgatc ccgtggacgg agtcgatgct ggtggactgc ctcttcctct
     1381 cctacctgct gggcggcacc atgaagtggg tccgcaccaa gcacggggag cgcatgcccc
     1441 cgctgcgcgc gctggaggat ttcgagatca cccgctcgga gaacccccag aaggtggccg
     1501 gacagctcta ccgggacttc ccgctggccc acgacatcac gttctcgtgc ttcacgctcg
     1561 atcgcgccca gggccgcaag ccgacgatgg acaactcctt cgacatcttc aagcggaacg
     1621 tggatcgggc ccggcgcgtc gtgcgcgacc tgtgacccgg gagaggaccg cgaggccatg
     1681 agcgcaggcg aagcccccat caagcaggcg gtgaagtgga tcgacgatca gctccacgac
     1741 gacccgaagg ccgaccgcac ccggctcatc gacgaggcgg gccggcgctt cgacctcacc
     1801 ccgctcgact ccgagttcct cctgcgccat ctctcccagc gtcacccctc ctgatccggg
     1861 cgacggccac gtgagcgaga gccagcaggc gcgggtccag gcccagttcg ggccgagcgc
     1921 ggccgcctac gtcaagagcc ccggtcacgc cgcgggcccc gacctggagc agctcatcgc
     1981 gtggggcaag aagcgcggcg cggcgcgggt cctcgacatc gccaccggcg gcggccacac
     2041 cgccctggcc ttctcgcggt tcacgccgag cgtggtggcg gtcgacgtca cgctgccgat
     2101 ggtccaggcc gcccgcgagt tcgcgaccgc cgagggcgcg acgggggtgc gcttcctggg
     2161 cgccgacgtc gaggccctcc cgttccgcga cggggccttc ggcaccgtga cctgccggac
     2221 cgcggcccac cacttcccgg ccctgctgcc cgcgctccgg caggtcgcgc gcgtgctccg
     2281 ggccggaggc tcgttcctgg tggaggacat ccagggccac gacgacgaag aagcctccgc
     2341 tttcatcttg gaggtcgagc ggcggcgcga tccgagccac gtgcgagcag ccttccgcca
     2401 cctcgagtgg accgcgttcc tgcgcgccgc cggcctcacc gtgatcgacg agggcggcat
     2461 gtcgaaggtc cgcgcctggg acgactggac gcggcgctcg cggatgacct ccgctgcgaa
     2521 ggcggagctc gaggcctacg tgctggctgc gcccgcgcgc tgccgggagg ccttcgactt
     2581 ccacatcgac ggcggtcgga tcgtctcgtt caccgaccgc atgctgctgc tgcgcgccga
     2641 ccgggactag gccttcagca ccaccaccgt cgcgccccag ccgccccggt cgggcggccc
     2701 gtcgaagaac cgctccaccg cgggatgccc ggcgagcacg ctccgcacga tggcccgctg
     2761 agtcccggtg cccttgccgt ggatcaggcg cacctcgcgg aagccgcgcg cggccgcctg
     2821 gcgcaggtac tcgtccacca ccgagcgtac gtcgcgcggg gcgaacgggt ggagatcgag
     2881 ggcgtcctcg atcggcagga tgatcggatc cggcccgggc tcgtccatgg cctcgccgct
     2941 agcctactcc agcccgccac tcgacccggt ccggcacttg acccagtccg gcacttgaca
     3001 aggtccgcgc ccgccccata atccgcgcat gtccgacgcc cgatcggaag cgctcgactg
     3061 gctctccacc ttcgaggccg cgtgccgcgg ccgggacttc gaggcgggcc gccgcctctt
     3121 cgcgcccgac gcggtggcct tcggcaccta cgccaccgcg gtccacggcc tcgacaacat
     3181 cgagcgcgag cagtggcgtc agatctggcc gcgcatccgc ggctttcgct tcgacgcgga
     3241 gcccgccgtc gcggggaccg gcgactcggc ctggatcgcg gccacctggg cgtcggaggc
     3301 gaccggcccg gacggccagc ccttcacccg ccacggccgg gccaccttca acctcgcccg
     3361 acgggacggc cggtggctgg cggtccacag ccacgtctcc ctgctcccga gccagtcgga
     3421 gagcgcccac gggcgccgct gaattcacac ggttgtgacc acgggcgcct ccgccgcccg
     3481 tagacttccg cgcaccgccc catcacgtcc cgacatggag ggaactggat gcgcacacac
     3541 gcagccggaa cacgggttct cgggctcgcc gtcgccatcg ccgtcctggc cacgctggcc
     3601 gcggtagtct cgcggagcgg cgcgggcgcg gacaagatcg agttccccgc ccggtacaag
     3661 gacggggtcc tctacacca
//
LOCUS       DATMCV010000079         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_270504, whole genome shotgun sequence.
ACCESSION   DATMCV010000079 DATMCV010000000
VERSION     DATMCV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270504"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VNU03_01940"
     CDS             <1..849
                     /locus_tag="VNU03_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWP73236.1"
                     /translation="WDINSDPLVRRLYILKQLATRQMWIGRLVHEAVERCLLALRDGH
                     GLSEASLIEDTVRQMRAEWKGSRAGLYRETPKRPSLFEHEYAVPIKDSEWQALRDHLV
                     RCLRNFHRLPVLTEIKRTPTERWIFIEDIGSFPFEGTRVFTAPDFGYWNSADRLQLLD
                     WKTGGGGEGAGLQLGGYALYALEVLGVDLARVDLLEVNLREGKVTSHPWDEASLDRVR
                     EHIRLSVRSMKAYLKDPDRNLAEESGFEKTEELRICRWCNFRSVCRPDMTPFAVPSPL
                     EGERAG"
     gene            867..1571
                     /locus_tag="VNU03_01945"
     CDS             867..1571
                     /locus_tag="VNU03_01945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP73237.1"
                     /translation="MKALLVDLDDTLLDYTGGVDECWHHACEAHAGPVGIDPAGLVKA
                     IVETRRWFWSDPGRHRTERTDMLRAWTRITGHALGRVGRADETLAAAIAADFSERRWE
                     RMALFPGVTTALGALRARGVPLALVTNGDASHQRRKIAQHDLERFFDVLVIEGEMGVG
                     KPDEIVYRYALSKLRVKAEEAWMVGDNFEWDVAAPQRLGLRGVWVDGPGQGLPEAADV
                     VPHRIIRTFPELLEPA"
     gene            1653..2240
                     /locus_tag="VNU03_01950"
     CDS             1653..2240
                     /locus_tag="VNU03_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73238.1"
                     /translation="MRDRNWLPMVMALVLVSTGCATQREWGVWRSHSTHFATDDHLAF
                     SATNAVAGAPRVTPELMDKAKGQGWWGRNIPSDVQPADVAGNWSGTWTGHGLLRSDRY
                     GVAQVSITQKGATGEGILVLQDSQATEGVPVALRENSSFGAPIEVAVSEKEMWVSGTQ
                     PSRPFAATFRVDGDRLIGTFLYMHSPVRIEMTRDR"
     gene            complement(2257..>2807)
                     /locus_tag="VNU03_01955"
     CDS             complement(2257..>2807)
                     /locus_tag="VNU03_01955"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HWP73239.1"
                     /translation="WRETRVVPGAQAQILMGSLAPALTDPDYPAVKVLSTVLGGGMAG
                     RFFSELRDKQGLAYTTGTQYPARVDTSFFLAQLGTAPENVAKAEAALREQLERVQRER
                     VGDEELRVAKAYVLGNLAMDRRTNARQAWYLAAYEEAGVGYEFLDRYVGAVRAVTAAD
                     LQRVAQRYLAVIRTIVVQPTPP"
BASE COUNT          421 a          983 c          978 g          425 t
ORIGIN      
        1 tgggacatca actcagaccc ccttgtccgt cgactctaca tcctcaagca gctcgccacc
       61 cgccagatgt ggatcggccg gctcgtgcac gaggcggtcg agcgctgcct cctcgctctt
      121 cgcgacggac acgggctgag cgaagcctcg ctcatcgagg acacggtgcg tcagatgcgc
      181 gcggagtgga agggctcgcg cgccgggctc taccgggaga cccccaagcg gccgtcgctg
      241 ttcgagcacg agtacgcggt gccgatcaag gacagcgagt ggcaggcgct ccgggaccac
      301 ctggtgcgct gcctgcggaa cttccaccgc ctgccggtgc tgaccgagat caagcgcacg
      361 cccaccgagc ggtggatctt catcgaggac atcggctcgt tccccttcga gggcacgcgg
      421 gtcttcaccg cccccgactt cggctactgg aactcggccg accgcctcca gctcctggac
      481 tggaagacgg gcggcggcgg ggaaggcgcc gggctccagc tcggcggtta cgccctctac
      541 gcgctcgagg tgctgggggt ggatctggcc cgcgtggacc tgctcgaggt caacttgcgg
      601 gagggcaagg tcacctcaca tccgtgggac gaggcgagcc tcgatcgggt ccgcgagcac
      661 atccgcctgt cggtccggtc catgaaggcc tacctgaagg acccggaccg caacctcgcc
      721 gaggagtcgg ggttcgagaa gaccgaggag ctgcgcatct gccgctggtg caacttccgg
      781 tcggtctgcc ggcccgacat gacccccttc gctgttccct ctcccctgga aggggagagg
      841 gcagggtgag ggtgaagggt cgctagatga aagccctcct ggtcgacctc gacgacaccc
      901 tgctcgacta caccggcggc gtggacgagt gctggcatca cgcctgcgag gcccacgcgg
      961 gccccgtcgg catcgatccg gccggcctcg tgaaggcgat cgtcgagacc cggcgctggt
     1021 tctggagcga tccgggccgc caccggaccg agcgcacgga catgctgcgc gcctggaccc
     1081 gcatcaccgg gcacgcgctc ggccgcgtgg gccgggccga cgagacgctg gccgcggcca
     1141 tcgcggccga cttctcggag cgccgctggg agcggatggc cctcttcccg ggtgtcacca
     1201 ccgcgctcgg ggccctccgc gcccgcggag tcccgctggc cctggtcacc aacggcgacg
     1261 cgagccacca gcggcgcaag atcgcccagc acgatctcga gcggttcttc gatgtcctgg
     1321 tcatcgaggg cgagatgggc gtcggcaagc ccgacgagat cgtctatcgc tacgcgctct
     1381 ccaagctgcg ggtcaaggcg gaggaggcgt ggatggtggg cgacaacttc gagtgggacg
     1441 tcgccgcccc tcagcgcctg gggctgcgcg gggtctgggt ggacggcccc ggccagggcc
     1501 tccccgaggc cgccgacgtc gtgccccatc gcatcatccg gacctttccc gagctcctgg
     1561 agcccgccta gaattcgctg ggtgataaat ccccggtatg caagactgtg attccacgag
     1621 tattttgtgg aatccgccgg agggaaacga tcatgcgaga tcgcaactgg ctgccgatgg
     1681 tgatggccct cgtgctcgtg tcgaccggct gcgcgaccca gcgcgagtgg ggggtctggc
     1741 gttcgcattc gactcacttc gccaccgatg accatctggc cttctcggcg accaacgcgg
     1801 tcgccggcgc accgcgggtc acccccgagc tgatggacaa ggccaagggc cagggctggt
     1861 ggggtcgcaa cattccttcg gacgtccagc cggccgacgt ggccggcaac tggagcggga
     1921 cgtggaccgg ccacgggttg ctgcggagtg accggtacgg ggtcgcccag gtgtcgatca
     1981 cccagaaggg ggccaccggc gaggggatcc tggtcctgca ggattcgcag gccacggaag
     2041 gcgtgcccgt cgccctgcgc gagaactcgt ccttcggcgc gccgatcgag gtggcggtct
     2101 cggagaagga gatgtgggtg agcgggaccc agccgagtcg gcccttcgcg gccaccttca
     2161 gggtggatgg agaccggctg atcgggacct tcctctacat gcactcgccg gtccggatcg
     2221 agatgacgcg ggaccgctga gccgcaccgc cccgcctcag ggcggggtgg gctggaccac
     2281 gatggtgcgg atcaccgcca gataccgctg ggccacccgc tggaggtcgg ccgcggtgac
     2341 cgcgcgcacc gctcccacgt aacgatccag gaactcgtag cccactcccg cttcctcgta
     2401 cgcggccagg taccacgcct gacgcgcgtt cgtccgccga tccatcgcga ggttgcccag
     2461 cacgtaggcc ttggcgaccc gcagctcctc gtcgcccacc cgctcgcgct gcacccgctc
     2521 gagctgctcg cgcagcgcgg cctcggcctt cgccacgttc tccggcgcgg tgccgagctg
     2581 ggcgaggaag aagctggtgt cgacccgcgc cgggtactgg gtcccggtgg tgtaggccag
     2641 gccctgcttg tcgcggagct ccgagaagaa gcggcccgcc atgccgccgc ccagcaccgt
     2701 gctcaacacc ttgaccgccg ggtagtccgg atcggtgagg gccggcgcga ggctccccat
     2761 caggatctgc gcctgggcgc cgggcaccac ccgcgtctcg cgccacg
//
LOCUS       DATMCV010000080         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_737721, whole genome shotgun sequence.
ACCESSION   DATMCV010000080 DATMCV010000000
VERSION     DATMCV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737721"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VNU03_01960"
     CDS             complement(<1..359)
                     /locus_tag="VNU03_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP73240.1"
                     /translation="MSQALTGIRVVDLTNNQAGPSCGQMLAWLGADVIKVEEPGRGDI
                     ARYSQKDRADADALFFLAFNANKRSLTLNLKHAGGQDVFRALLKTADVLLENFGPGVI
                     ERLGFGYEDVKRVNPRIV"
     gene            466..1365
                     /locus_tag="VNU03_01965"
     CDS             466..1365
                     /locus_tag="VNU03_01965"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP73241.1"
                     /translation="MATTRTAGAVSFRQVGPCLAAEVEGIDLTQPLSPEEVAAVHAGM
                     DKYAVLVFHDQPLTDEQQLAFTRSLGEIEHAIGTGLREQYRLPTTFADVSNLDVNNQV
                     FARDSRTRLFSLGNRLWHSDSSFKVVPAKYSLLHARSVPSKGGNTEFADMRAGYDALD
                     QETKDRVADQICEHSQLYSRALLGFTDFTDEERERFKPVRQVLVRTHPGHGRKSLYLS
                     SHAGGIVGWPVPEARAFLRDLNEIATQRQFVYSHQWRVGDLVMWDNRQTMHRSRPFPE
                     HEPRDVRRTTLQGAGPTTEQVPA"
     gene            1365..>2099
                     /locus_tag="VNU03_01970"
     CDS             1365..>2099
                     /locus_tag="VNU03_01970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP73242.1"
                     /translation="MKFGLFFQAPEAPGQSHADRYTEMIDLIGLADTLGFDVAWLAEL
                     HFGGAFSLLSNPLMAVPVIAQRTRRIRIGTAVTLLPLHHPLACAEQAATADLLSGGRL
                     EFGVGRGSIPSQFHGFRVPVSENRGRFGEALDIIRLAWTRERFSYDGTYYQVEDVSVV
                     PRPLQQPHPPIRVAVHSGESFAHIGGLGLPIYSGTTTTPLPQLREFMAVYRARLAEAG
                     HPWQPDQMALMLPVHIGETSAAARDAM"
BASE COUNT          303 a          769 c          721 g          306 t
ORIGIN      
        1 acgatgcgcg gattgacgcg cttgacgtcc tcgtagccga agcccagccg ctcgatgacg
       61 ccgggcccga agttctcgag cagcacgtcg gcggtcttga gcagcgcgcg gaagacgtcc
      121 tgcccgcccg cgtgcttcag gttgagcgtc aggctccgct tgttggcgtt gaaggcgagg
      181 aagaagagcg cgtcggcgtc cgcccggtcc ttctgggagt agcgcgcgat gtcgccccgg
      241 cccggctcct ccaccttgat cacgtccgcc ccgagccacg ccagcatctg gccgcacgag
      301 ggtcccgcct ggttgttcgt gagatcgacg acgcgaatgc cggtcagggc ttggctcata
      361 ggtcccatgc ctaccacagc cgctccgcgc ggtcgaggca ccccgcgccc cggtgcgcgc
      421 cgggccccct tgactacgga cggacgggcg gcgcaatctc ccggcatggc gaccacacga
      481 acggccggcg cggtgagctt ccgccaggtg ggaccctgcc tggcggccga ggtcgagggc
      541 atcgacctga cccagcccct ctccccggag gaggtggcgg cggtgcacgc gggcatggac
      601 aagtacgcgg tgctcgtctt ccacgaccag ccgctgaccg acgagcagca gctcgccttc
      661 acccggagcc tgggcgagat cgagcacgcg atcggcaccg gcctccgcga gcagtaccgg
      721 ctgccgacca ccttcgccga cgtgtcgaac ctggacgtga acaaccaggt cttcgcgcgc
      781 gacagccgca cgcggctctt ctcgctcggc aaccggctct ggcactccga cagctcgttc
      841 aaggtggtcc ccgccaagta ctcgctcctg cacgctcggt ccgtcccgtc gaagggcggc
      901 aacaccgagt tcgcggacat gcgcgcgggc tacgacgcgc tcgatcagga gacgaaggac
      961 cgggtggccg accagatctg cgagcactcc cagctctact cgcgcgcgct gctcgggttc
     1021 accgacttca cggacgagga gcgcgagcgc ttcaagccgg tgcgccaggt cctggtccgc
     1081 acccatcccg gtcacgggcg caagtcgctc tatctctcct cgcacgcggg cggcatcgtg
     1141 ggctggccgg tgccggaggc gcgcgcgttc ctccgcgacc tcaacgagat cgccacccag
     1201 cgacagttcg tctactccca ccagtggcgg gtgggcgacc tggtgatgtg ggacaaccgc
     1261 cagaccatgc atcgctcgcg gcccttcccg gagcacgagc cgcgcgacgt gcgccgcacc
     1321 acgctccagg gcgccgggcc gaccaccgag caggtcccgg cttagtgaag ttcggactgt
     1381 tcttccaggc tccggaggcg ccgggacaga gccacgccga tcgctacacc gagatgatcg
     1441 acctcatcgg cctggccgac acgctgggct tcgacgtggc gtggctggcc gagctgcact
     1501 tcggcggcgc cttctcgctg ctgtcgaacc cgctgatggc ggtgccggtg atcgcgcagc
     1561 gcacccggcg catccgcatc ggcaccgcgg tgaccctgct gccgctccac cacccgctgg
     1621 cctgcgcgga gcaggccgcg accgccgacc tcctgtccgg cggccggctc gagttcggcg
     1681 tcgggcgtgg gtcgatcccg agccagttcc acggcttccg ggtgccggtg tccgagaacc
     1741 gcggccgctt cggcgaggcg ctcgacatca tccggctggc ctggacgcgc gagcggttca
     1801 gctacgacgg cacctactac caggtggagg acgtgtcggt ggtgccgcgg ccgctgcagc
     1861 agccgcaccc gccgatccgg gtggcggtgc actccgggga gagcttcgcg cacatcgggg
     1921 gcctggggct gccgatctac tcggggacga ccaccacgcc gctgccgcag ctgcgcgagt
     1981 tcatggcggt ctaccgcgcg cggctggccg aggccgggca cccgtggcag cccgaccaga
     2041 tggcgctgat gctgccggtg cacatcggcg agacgagcgc agccgcgcgg gacgcgatg
//
LOCUS       DATMCV010000081         3522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_336518, whole genome shotgun sequence.
ACCESSION   DATMCV010000081 DATMCV010000000
VERSION     DATMCV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336518"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VNU03_01975"
     CDS             <1..522
                     /locus_tag="VNU03_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP73243.1"
                     /translation="GAELAGHPAYEIVAHGIAHVPEGRRLFPQLTVAENLKMGAFLPS
                     ARAHFRESLERVYALFPVLAERPTQRAGSLSGGEQQMLAVGRALMSRPKLILFDEPSM
                     GLAPVMVLRLFDLIRRVREEGYTILVVEQNVRQVLKLVDRAYLLEVGRIKIEGRAADL
                     AEQDFIRQAYVGL"
     gene            526..1392
                     /locus_tag="VNU03_01980"
     CDS             526..1392
                     /locus_tag="VNU03_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73244.1"
                     /translation="MLDPIFILEAVVNGILLGGILALLALGLNLIFGVIDIVWIAYVD
                     LVTLCMYGVYFLVSLYGWPLWLAGLAGIGIGALLGVLVHLLIITPILASPPINQLLAT
                     GGLLFFIQSAATLLWSTDHRSLRVSLPTLEIGGIFISFTRLLAFGLAILAMVALYLFL
                     TRTYTGTAIRAVSQDREAIALMGARPQRLYLVTSAVGGAMAGVAGALLIIQYAVHPYF
                     GGAFGPLTFMICVLGGLGNMVGAFAAAFIMAQVISVGGVLVSTEMGYVIAFVIFIVLM
                     FVRPGGIFARRT"
     gene            1389..2393
                     /locus_tag="VNU03_01985"
     CDS             1389..2393
                     /locus_tag="VNU03_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73245.1"
                     /translation="MSAARVLGAAGLVALALVPLLPLGPAQEYIWHVIIQIFLWSFVG
                     NAWSLMGRFGLVSLGHGAFLGMGAYATVLLWNFYGMTPWAGAGLAILMTVAFALVVAY
                     PCSRFQVVGHYFGLVTLAVGEVIRLLIVAERDWTGGSLGVTLKTGGAASDWWTLQFAD
                     KRVFYYVGLAAWLFGLWVWWRVDRSMARSAMEAIGEDETAAASVGIRVARFKMAITAL
                     SAGLTALGGALLALYLAYANPDSLAGIGVSLRIVFAVVLGGMYALLGPFVGTALTIAL
                     SEYLRIYFGIKLIGIAETIYGLLLIVFIIFLPAGIYGSIAQAVRRRLTRPSGGLPARA
                     "
     gene            2413..2955
                     /locus_tag="VNU03_01990"
     CDS             2413..2955
                     /locus_tag="VNU03_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73246.1"
                     /translation="MVTLASECRLQPNVRGWLGAAVVAMVILGGCATEGGIDLRGGQT
                     AKQLEEDRAKCLPFVQAHTEITADVAEAACLVGRGYGAPVTFAQGPARIGYLYATSRA
                     EAPTVAADFQGCQVEAFKAPVPVIPDTNTSGIFSNFFSKVFPRGFTSKPPTPDDWAFK
                     AFAGCLSRRGYTVTDVTRLK"
     gene            complement(2980..>3522)
                     /locus_tag="VNU03_01995"
     CDS             complement(2980..>3522)
                     /locus_tag="VNU03_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWP73247.1"
                     /translation="ERRVAEANEQIGIARAAYYPTVMLNASVGFEGSSFGNFLNASSL
                     LWAVGTSITQTIFDGGRRRATSEAALAAYDATVAGYRQTTLTAFQQVEDNLVALRVLE
                     QEAQQQRRAVESAQLSLQLFTNRYRGGVDNYLQVITAQTVALQNQRNEIDILRRRIDA
                     SVLLVKALGGGWTLADLPAE"
BASE COUNT          429 a         1275 c         1193 g          625 t
ORIGIN      
        1 ggcgcggagc tggccggcca ccccgcctac gagatcgtgg cccacggcat cgcgcacgtg
       61 ccggagggcc ggcgcctctt cccccagctc accgtcgccg agaacctcaa gatgggcgcg
      121 ttcctgccgt cggcgcgcgc gcacttccgc gagagcctcg agcgcgtcta cgcgctgttc
      181 ccggtgctgg ccgagcgccc gacccagcgg gcgggcagcc tgtcgggcgg cgagcagcag
      241 atgctcgcgg tgggccgcgc cctcatgtcg cgccccaagc tgatcctctt cgacgagccg
      301 tccatgggcc tggcgccggt gatggtgctg cgcctcttcg acctgatccg ccgcgtgcgg
      361 gaggaaggct acacgatcct ggtggtcgag cagaacgtcc gccaggtgct caagcttgtg
      421 gaccgcgcat atcttctgga agtcggccgc atcaagatcg agggccgcgc cgccgacctc
      481 gccgagcagg acttcatccg ccaggcctac gtggggctct gacccatgct cgatccgatc
      541 ttcatcctcg aggcggtggt gaacgggatc ctgttgggcg gcatcctcgc cctcctcgcc
      601 ctcggcctca acctgatctt cggcgtcatc gacatcgtct ggatcgccta cgtggacctg
      661 gtcaccctgt gcatgtacgg ggtctacttc ctggtgagcc tctacggctg gccgctctgg
      721 cttgccgggc tcgccggcat cgggatcggc gcgctcctgg gcgtgctggt gcacctgctg
      781 atcatcacgc cgatcctggc gagcccgccg atcaaccagc tgctcgccac cggcggcctc
      841 ctcttcttca tccagtcggc cgccacgctg ctctggtcca cggaccatcg ctccctgcgg
      901 gtgagcctgc ccacgctcga gattggcggc atcttcatct cgttcacccg cctcctcgcc
      961 ttcggcctcg cgatcctggc catggtcgcg ctctacctct tcctcacccg cacctacacc
     1021 ggcaccgcga tccgcgcggt gagccaggac cgcgaggcca tcgcgctgat gggcgcgcgg
     1081 ccgcagcgcc tctacctggt gacctccgcg gtgggcgggg cgatggcggg cgtcgcgggc
     1141 gcgctgctga tcatccagta cgcggtgcac ccctacttcg ggggggcctt cggcccgctc
     1201 accttcatga tctgcgtgct gggcggcctg gggaacatgg tgggggcctt cgcggccgcg
     1261 ttcatcatgg cccaggtgat ctcggtgggc ggcgtgctgg tctcgaccga gatgggctac
     1321 gtcatcgcct tcgtcatctt catcgtcctg atgttcgtcc gcccgggcgg catcttcgcg
     1381 aggcgcacgt gagcgcggcc cgggtcctgg gcgcggccgg cctggtggcc ctggccctgg
     1441 tgccgctgct cccgctcggg ccggcccagg agtacatctg gcacgtcatc atccagatct
     1501 tcctctggtc cttcgtgggc aacgcatggt cgctgatggg ccgcttcggc ctcgtctccc
     1561 tgggccacgg tgcgttcctc ggcatgggcg cctacgccac cgtgctcctc tggaacttct
     1621 acgggatgac cccgtgggcg ggggccgggc tcgccatcct gatgacggtg gccttcgccc
     1681 tggtggtggc ctatccgtgc tcgcgcttcc aggtggtcgg ccactacttc ggcctcgtca
     1741 ccctcgcggt cggcgaggtg atccggctcc tgatcgtggc cgagcgcgac tggaccggcg
     1801 gctcgctcgg ggtgaccctc aagacgggcg gcgccgcgtc cgactggtgg acgctccagt
     1861 tcgcggacaa gcgcgtcttc tattacgtgg gcctcgccgc ctggctcttc gggctctggg
     1921 tgtggtggcg ggtggatcgc agcatggccc gctcggccat ggaggccatc ggcgaggacg
     1981 agaccgcggc ggcctcggtg ggcatccggg tagcccggtt caagatggcg atcaccgcgc
     2041 tctccgcggg cctcaccgcg ctgggcgggg cgctcctcgc gctgtacctg gcttacgcca
     2101 accccgacag cctcgcgggc atcggggtgt ccctgcgcat cgtgttcgcg gtggtgctcg
     2161 gcggcatgta cgccctgctc ggtcccttcg tggggaccgc gctgaccatc gcgctcagcg
     2221 agtacctgcg catctacttc ggcatcaagc tcatcgggat cgccgagacc atctacgggc
     2281 tcctcctgat cgtgttcatc atcttcctgc ccgccggcat ctacggcagc atcgcgcagg
     2341 cggtgcgccg tcgcctgacc cggccttccg gcggcctccc ggctcgggca tagtgaatcc
     2401 cggaccgatg acatggtgac cctggcgagc gagtgcaggt tgcagccgaa cgtgcgcggg
     2461 tggctcggag cggccgtggt ggcgatggtg atcctgggcg gctgcgcgac cgagggcggt
     2521 atcgacctgc ggggcggcca gactgccaag cagctcgagg aggatcgcgc caagtgcctg
     2581 ccgttcgtcc aggcccacac cgagatcacc gccgacgtcg cggaggccgc gtgcctggtc
     2641 gggcgcggct acggcgcgcc ggtgaccttc gcgcagggcc ccgcgcggat cggctacctg
     2701 tacgcgacct cccgcgccga ggcgcccacg gtcgcggccg acttccaggg ctgccaggtc
     2761 gaggccttca aggcgccggt cccggtgatc ccggacacga acacctccgg gatcttctcg
     2821 aacttcttct ccaaggtctt cccccgcggc ttcacctcca agccgccgac cccggacgac
     2881 tgggccttca aggccttcgc gggctgcctg agccgccgcg gctacacggt caccgacgtc
     2941 acccggctca agtgagccgg tagccggcgt cgccggcgct cactcggccg gcaggtcggc
     3001 cagggtccag ccgcctccga gggccttcac gagcagcacg ctggcgtcga tccgccggcg
     3061 gaggatgtcg atctcgttgc gctggttctg gagcgcgacg gtctgcgcgg tgatcacttg
     3121 caggtagttg tcgaccccgc cccggtaacg attcgtgaag agctggagcg agagctgggc
     3181 cgactccacc gcccgccgct gctgctgcgc ctcctgctcg agcacgcgga gcgcgaccag
     3241 attgtcctcc acctgctgga acgcggtcag agtggtctgc cggtagcccg cgacggtggc
     3301 gtcgtaggcg gccagcgccg cctccgaggt ggcccgccgt cgcccgccgt cgaagatggt
     3361 ctgggtgatc gacgtgccca ccgcccagag caggctcgac gcgttcagga agttcccgaa
     3421 gctcgagccc tcgaacccga ccgacgcgtt cagcatcacg gtcgggtagt aggcggcgcg
     3481 ggcgatgccg atctgctcgt tggcctccgc gacccgccgc tc
//
LOCUS       DATMCV010000082         4741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_138181, whole genome shotgun sequence.
ACCESSION   DATMCV010000082 DATMCV010000000
VERSION     DATMCV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138181"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VNU03_02000"
     CDS             <1..762
                     /locus_tag="VNU03_02000"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWP73248.1"
                     /translation="LSTDEKVELLDRLVAGGIDPTRLMPGTGCCALTDSVRLTAHAMK
                     LGAGGVLMLPPFYYKGVSDDGLFKSFAEVIERVGDRRLRLYVYHIPPVSQVPITLGLI
                     ERLLKAYPDVVAGVKDSSGDWSNTKAMLDAFAAGGFDVFAGSETFLLANMRGGGAGCI
                     SATANVNPAAIARLFREWQTPEADAQQARLDEIRGIMQRYPMIPALKAVIARYGGDAA
                     WVTVRPPLVALTAEQRASLAAELGRAAFTMPGLGE"
     gene            767..1606
                     /locus_tag="VNU03_02005"
     CDS             767..1606
                     /locus_tag="VNU03_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWP73249.1"
                     /translation="MKELAARLDALCDALPFQTSWYLKDLATGARADRLGDVPVPSAS
                     TRKISIMMAALKAVHDGKLALDQKVVIDAKYQDNDSGTFQHLTPGFWITFRDALVMMI
                     IVSDNTCTGQVVDLVGLGEIQRFCESVGMTRTVHRFGIPPRLGPDHALDQVTTTTPND
                     QGMLLELILRGTTDAAVAARMGVTPELCRLGLDILSWQKLKARLPSQLPLGTKIAHKT
                     GTGSRGFMDAGIVFKADRPLFILTAYTEHVPAALPDGTPGFTAANTLIGRMARVSWDT
                     LGG"
     gene            1618..2358
                     /locus_tag="VNU03_02010"
     CDS             1618..2358
                     /locus_tag="VNU03_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWP73250.1"
                     /translation="MPAKTQPKEPPAPKRAMSIHAHPDDQEFTVGGTLAKWARAGCEV
                     VTVCITSGGAGSNKYTPLDMTRHALVALREDEQREACQLLGVKETIFLRYEDGMLEPS
                     IALRKDLTRLIRRFRPEVVLTGDPTVRFYGSTYMNHPDHRVAADVALDAVFPSAGTRL
                     IFPELLDEGLEPHEVRQVFIHGAERADTYVDIGEVLDVKLAALRAHKTQMGEWDPTEM
                     ITQWAREQGKRRKLAAAESYRRMMLQDS"
     gene            complement(2373..2783)
                     /locus_tag="VNU03_02015"
     CDS             complement(2373..2783)
                     /locus_tag="VNU03_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73251.1"
                     /translation="MKRRLLGWTAAVIVGALLLGVFAAGDALAWDRGRGGFHGGFHGH
                     RGFFGPRVFVGPRFYGPGFYPYYGYAYPYAYPYPYYDYPPPVVVTPPPTDYVQRAPST
                     PTSGYWYYCQDPGGYYPTVAACPGGWIQVAPRSE"
     gene            2931..3209
                     /locus_tag="VNU03_02020"
     CDS             2931..3209
                     /locus_tag="VNU03_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73252.1"
                     /translation="MTLPVEMARDKKPASRTLKKKLVFLGVLALVVWFACGALTDWYH
                     GYLVNRCIRELGINYRSQSAPCEQFWRRGGLPWVGFSDLLSRPREAVR"
     gene            complement(3992..>4741)
                     /locus_tag="VNU03_02025"
     CDS             complement(3992..>4741)
                     /locus_tag="VNU03_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HWP73253.1"
                     /translation="STVHKATRPNYNESFFISHERAADHPDVLAGTPLRGRNQWPEGH
                     AAMRAAMVAYFTTLQGVGERMLPVLARALGMPAGHFAPSFAGEAHINLRFLHYPPQDI
                     ADDEQFGQGPHTDNSFITILARMDVPGLAVRLPSGEWLAPPLIPGTFLVNLGNMMKRW
                     SNDRFLSTPHAVLNESGTDRYSIAFFYSPTPAAVIECLPSCTGPDDPPHYPPAVYGDL
                     VVDFYRANYFHQKGHQRADHAEVSETPGANY"
BASE COUNT          787 a         1598 c         1599 g          757 t
ORIGIN      
        1 ctctcgaccg acgagaaggt cgagctgctg gatcggctgg tcgcgggcgg gatcgatccc
       61 acccggctca tgccggggac cggctgctgc gcgctcaccg actcggtgcg gctcaccgcg
      121 cacgcgatga agctgggcgc gggcggggtg ctcatgctgc cgcccttcta ctacaagggc
      181 gtctccgatg acgggctctt caagagcttt gcggaggtga tcgagcgagt gggcgaccgg
      241 cggctgcggc tgtacgtcta ccacatcccg ccggtgtcgc aggtcccgat caccctcggc
      301 ctgatcgagc gcctcctcaa ggcctatccc gacgtggtgg ccggcgtgaa ggacagctcg
      361 ggcgactgga gcaacaccaa ggcgatgctg gacgccttcg cggcgggagg cttcgacgtc
      421 ttcgcgggga gcgagacctt cctgctcgcg aacatgcgcg gcggcggcgc gggctgcatc
      481 agcgccaccg cgaacgtgaa cccggccgcc atcgcgcgtc tctttcggga gtggcagacc
      541 ccggaggcgg acgcccagca ggcgcgcctc gacgagatcc gcggcatcat gcagcgctac
      601 ccgatgatcc ccgcgctcaa ggcggtcatc gcgcgctacg gtggcgacgc ggcgtgggtc
      661 acggtgcgcc cgcccctcgt ggccctgacc gcggagcagc gagcgtcgct ggccgccgag
      721 ctggggcgcg ccgccttcac gatgccgggg ctgggggagt agcgccatga aagagctggc
      781 cgcccgcctc gacgccctct gcgacgcgct cccgttccag accagctggt atctcaagga
      841 tctggccacc ggcgcccgcg ccgatcggct cggcgacgtg ccggtgccct cggccagcac
      901 gcgcaagatc tcgatcatga tggccgcgct caaggcggtc cacgacggca agctcgccct
      961 ggaccagaag gtggtcatcg acgcgaagta ccaggacaac gactcgggga cgttccagca
     1021 cctgaccccc ggcttctgga tcaccttccg cgacgcgctg gtgatgatga tcatcgtcag
     1081 cgacaacacc tgcaccggtc aggtggtgga tctggtcggc ctcggcgaga tccagcgctt
     1141 ctgcgagtcg gtcggcatga cccgcaccgt gcatcgcttc ggcatcccgc cgcgcctcgg
     1201 ccccgaccac gcgctcgacc aggtgacgac caccacgccc aacgatcagg gcatgctgct
     1261 cgagctgatc ctgcgcggca ccaccgacgc cgcggtcgcc gcgcgcatgg gcgtgactcc
     1321 cgagctgtgt cggctcgggc tcgacatcct gtcctggcag aagctcaagg cgcggctgcc
     1381 ctcgcagctc ccgctcggca ccaagatcgc ccacaagacc ggcaccgggt cgcgcggctt
     1441 catggacgcg ggcatcgtgt tcaaggcgga ccggccgctg ttcatcctga ccgcctacac
     1501 cgagcacgtg cccgccgcgc tgcccgacgg cacccccggc ttcaccgccg ccaatacgct
     1561 gatcgggcgg atggcgcgcg tgagctggga cacgctcggc ggctaggtct ccggcccgtg
     1621 cccgccaaga cgcagccgaa ggagccgccc gcccccaagc gggcgatgag catccacgcg
     1681 catcccgacg accaggagtt cacggtcggc ggcaccctcg ccaagtgggc gcgcgccggc
     1741 tgcgaggtcg tcacggtgtg catcaccagc gggggcgccg gctccaacaa gtacacgccg
     1801 ctcgacatga cccgccacgc ccttgtcgcc ctgcgggagg acgagcagcg ggaagcgtgc
     1861 cagctcctgg gggtgaagga gacgatcttc ctccgttacg aggacggcat gctcgagccg
     1921 tcgatcgccc tgcgcaagga cctgacccgg ctgatccggc gcttccggcc ggaggtggtg
     1981 ctgaccggcg accccaccgt gcgcttctac gggtcgacct acatgaacca cccggatcat
     2041 cgggtcgccg ccgacgtcgc gctcgacgcg gtcttcccct ccgcgggcac gcgcctgatc
     2101 tttcccgagc tgctcgacga gggcctcgag ccgcacgagg tgcgccaggt cttcatccac
     2161 ggcgcggagc gggccgacac ctacgtggac atcggagagg tcctcgacgt gaagctggcc
     2221 gcactgcgcg cgcacaagac ccagatgggc gagtgggatc ccaccgagat gatcacccag
     2281 tgggcccgcg agcagggcaa gcgccggaag ctggccgccg ccgaatccta ccggcggatg
     2341 atgctgcagg actcctgagc tactcggatg agctactcgg agcggggcgc gacctggatc
     2401 cacccgccgg ggcacgcggc caccgtcgga tagtagccgc ccggatcctg gcagtagtac
     2461 cagtagccgg aggtcggcgt gctgggcgcg cgctggacgt agtcggtcgg cgggggcgtc
     2521 acgacgaccg gcggcggata gtcatagtag ggatagggat acgcataggg atacgcgtac
     2581 ccgtagtacg ggtagaagcc gggcccgtag aaccgcgggc ccacaaacac gcgcggaccg
     2641 aagaagcccc ggtgcccgtg gaagccgccg tggaatccgc cccgcccacg atcccaggcc
     2701 aacgcgtcac ctgccgcgaa cacccccagc aggagcgcgc ccacgatcac ggccgcggtc
     2761 catccgagca gccgcctctt catgcctcaa gtgtccaccc gccgggagcg ggtgtctagc
     2821 cgtacgtcgt gagcagcacg tgcagacgct tctgccgatc gcgaagcgga cactgaagaa
     2881 taaattccga ggcgctacga ttgaagcagc cggtcagagg agggcaacca atgacactcc
     2941 ccgtcgagat ggcccgcgac aagaagcccg catcgcgcac gctcaagaag aagctcgtct
     3001 tcttgggtgt cttggctctg gtggtctggt ttgcttgcgg cgcgctcacc gactggtacc
     3061 acggctactt ggtgaaccga tgcattcgcg agctagggat caactaccgg agccagagcg
     3121 caccgtgcga gcagttctgg cggcgaggag gtcttccctg ggtgggattc agcgacctcc
     3181 tcagtcgacc acgagaagct gttcgttgat cggttccgga agggttgcat caacagtatg
     3241 ggcgcatcgg actgggctag cttggtgacg atgaacgtgc cccggccgag atgggtcacg
     3301 gcggcggcct tctggtcagg ctcgaaccac caggcgtcct aagtcgccgt ccctcgaagt
     3361 agcccacccg cctcaccatc cgttcccttc gggggcggca tggtggccaa tcgctctctg
     3421 gacatcgcgc acacacattc gccctataaa ccttccgccg ttagaagcgc ctcaaggtca
     3481 ctctcatggc gaagcgcctc cagagctgca ggatcatagg cgcagtcctc gatgcgtttc
     3541 atggcccgct ctcgatcttt gttcgccacg tagatacagg gtggctaaac gcagtatcgc
     3601 ctctctcacc aagtcccctg ttcttggatc ggagatcgag cagtagattt cgccaggcag
     3661 tgacaatttg atgcaggagg gcgcgagcac tcggcgcggg tgtcggaggc atcgcccgtg
     3721 agtctgccag cgtcgaggag cgagccggag gtgcggcgtg tccttactgg gtaaggattg
     3781 gtcactgcca gtacttcctc ggatcggaac tccgttgatc cttcgtacac tggtccgctg
     3841 tcaacggtgc ggattggtct cgaacgggta agtgatcagg tcgggcagat ttcttgccca
     3901 acgaacgaag ggcgcgagct tgatgacctg agcccgcgcc ctcgactgct tgcaccaagg
     3961 gtgctcgacg tcgcccctcg gtgcagtctg gttagtagtt cgctcctggc gtctcgctca
     4021 cttccgcgtg atctgctcgc tgatgacctt tctggtggaa gtagttggcc cggtagaaat
     4081 cgaccaccag atccccgtag accgcgggcg gatagtgcgg cgggtcgtcg gggccggtac
     4141 agctcggcag gcactcgatc accgcggccg gagtcgggct gtagaagaac gcgatcgagt
     4201 agcggtcggt cccggactca ttcagcaccg cgtggggggt ggagaggaac cggtcgttcg
     4261 accaccgctt catcatgttc cccaggttga ccaggaaggt ccccgggatc agcggcgggg
     4321 cgagccattc ccccgacggc agccgcaccg cgagcccggg cacgtccatc cgggccagga
     4381 tcgtgatgaa cgagttgtcg gtgtgcgggc cctgtccgaa ctgctcgtcg tccgcgatgt
     4441 cctggggcgg gtagtgcagg aaccgtagat tgatgtgggc ctcgcccgcg aaggacggcg
     4501 cgaagtgacc ggcgggcatc ccgagggcac gggccagcac cggcagcatg cgctcgccca
     4561 cgccctgcag cgtggtgaag taggcgacca tcgccgcgcg catggcggcg tgtccctccg
     4621 gccactggtt gcggccgcgc agcggggtgc ccgccagcac gtccgggtga tcggcggccc
     4681 gctcgtggct gatgaagaag ctctcgttgt agttcgggcg ggtcgccttg tggacggtgg
     4741 a
//
LOCUS       DATMCV010000083         8174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_138197, whole genome shotgun sequence.
ACCESSION   DATMCV010000083 DATMCV010000000
VERSION     DATMCV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138197"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..743
                     /locus_tag="VNU03_02030"
     CDS             189..743
                     /locus_tag="VNU03_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HWP73254.1"
                     /translation="MLHAIMDFVVDQYFPIVDSLEDDLETLEAHIFGDTVSRDTTMRI
                     YTLKRDLLEIKRGVSPLVDICNRLMRTDLDLIPDDARPYFRDVYDHAIRLSEKVDGLR
                     ELLGTALEANLSLTTIAQNEATKRITGWAAIFAIPTVIAGVYGMNFEFMPELRWRWGY
                     PVVMTVTVSLCGILYYRFKRSGWL"
     gene            760..1728
                     /locus_tag="VNU03_02035"
     CDS             760..1728
                     /locus_tag="VNU03_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWP73255.1"
                     /translation="MRVLVMGGTEFISLHLLRALQARGHEVTVFNRGRQPDRLPAGVT
                     VIAGDRKDHAGLRERLAGLRFDAVYDVTYAPTLGEDVAALVDALAGAPHVIFVSTCRV
                     YDHALPIPYSEETARGLYWGDYARHKIGGEDVLLERHRRDGLPVTIVRPTHVMGPLNT
                     RNNETFFMDRIHRGRPVLVPGHGGWLRQFGHVEDLAEVMAALPCDARAHGQAYNVMGE
                     DVVSQVGFVELVAEAMGRPVTFRHFDPALLKSFAKPGPVFGQNLVYDCHAVHTTTKLR
                     ADLGLRPRYTLHAGLVQTWEWYRAQGLADRPVDFSFEDQILSKIGA"
     gene            1725..2669
                     /locus_tag="VNU03_02040"
     CDS             1725..2669
                     /locus_tag="VNU03_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HWP73256.1"
                     /translation="MTRRILVYHPEPTDTRAYAELIRLPSRGWAVTAALTPEAAIASA
                     PEAEVLYCWRPPPELLAAATRLRWIQCMGAGVEQLLVPEVSPRVTITRVAGIFGPWMA
                     EYALGWCLWVTQRMDRFRADQRARRWAPVDPIPLRGQTLCVVGLGDIGARIARSGRAL
                     GLRVLGVSRSGRAVPPVERVYRTGALRAALRLADFVVLTLPLSAATRGMLGPPALAAM
                     KPSAWLINVARGPIVDEAALLDALRARRIGGAILDVFDTEPLPPEHPLWAFDNVVITP
                     HISGPSTPREIAPIFADNLRRYLAGRPLRNAVDRRRGY"
     gene            2669..3241
                     /locus_tag="VNU03_02045"
     CDS             2669..3241
                     /locus_tag="VNU03_02045"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HWP73257.1"
                     /translation="MATIEALWAAWDQPGLEHLRLRVDAGGARADSVIIALDDDGRPF
                     RARFAIETDPDWRVRHARIELLGDSARALDLHADGLGHWTDAVTGAAQPLDGCLDIDV
                     YPSPFTNTLPIRRLPALAPGAPVAIAVAYVTLPELTVRPAPQEYILLERQPDGARWRF
                     RSLDSGFTAELPVDAEGLVRDYPGIARRLR"
     gene            complement(3238..4284)
                     /locus_tag="VNU03_02050"
     CDS             complement(3238..4284)
                     /locus_tag="VNU03_02050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP73258.1"
                     /translation="MPVRAPGEAVGAAALPTVEAPCFLCGAADARPLWATPDRAFSVP
                     GVYTVARCDRCGFLYQRPRVRDEHLADCYPDHYPRHQEPSPRIPFKGGSARVRAVRWA
                     LASELGYTVWRAAGAGLLTRLRARLLLRRLRWDCPAWRGRGRYLDVGCGSGGALGVAA
                     ALGWHVAGVEVDAAAAAKARRFTPRVHTGDLQAAPFAAGEFDLITALHVLEHVPDPVG
                     AVRRMLGWLAPGGLLVIEVPNAGGLGARIFGRAWSGLELPRHLSHFTPATLERAAAQA
                     GGRVVWCWHQAKPRYYLWSLGYWLRDHRASWLARVAEWRPVYGALKLALELTLPLARW
                     TQRGEVIRVGIERA"
     gene            complement(4284..6077)
                     /locus_tag="VNU03_02055"
     CDS             complement(4284..6077)
                     /locus_tag="VNU03_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HWP73259.1"
                     /translation="MAGGVGRYTGPAMIVLGITETHCATAAVLRDGRIVGCASEERFS
                     RLKNDAGYPRRAIDALLRELDIRPDQIDLVALAGARAASREWLNRVLHDEDYAREYYG
                     VAWPTRRRAFEKKVRKWGAKFGLIDASRGKFGISQRERLGAVTGHLGIGADRIACLDH
                     HTCHAAAAYWGSGFAGRPTLVLTNDNSGDGLCATASTGSGLTLDRHEATPSAPGSLGA
                     FYSFATLALGMKFGEHEYKVMGMAPYAPDKYARRAEEALRAVFDLEEGRPARFRWRAP
                     GERYQLLLRAMVGLRFDWVAGGAQRLLEDVLLRWTRLMRERYGGGRLALGGGVFMNVK
                     ANMLIGQEEWVEELFAFPSCGDESNAVGAAYLGYLKECARRGVPAAPQAFGPAYLGPS
                     VTDEEAEQVIRERGLEGQYKVAYHDRIEERIAELLVSDGVVARCAGRMEFGARALGNR
                     SILANPSDHRVVGLINRMIKNRDFWMPFAPTVLAERAGDYLVNPKDLASPYMMLAMPT
                     RPEAREALAAALHPQDATARPQILEREWNPEYHAVIREFERRTGVGAVLNTSFNLHGE
                     PIVGSAADAIDTFERSGLPHLAVGHWLLSKK"
     gene            complement(6038..7387)
                     /locus_tag="VNU03_02060"
     CDS             complement(6038..7387)
                     /locus_tag="VNU03_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008279745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP73260.1"
                     /translation="MSSAELWRLSATEAAGLIRAGTIESGELVAACLERVTRVEPDVQ
                     AWTFLDPDHAMAQARAADEWRRSGRPLGAPHGVPVGLKDIIDTADMPTENGSVLHAGR
                     TPSRDAAVTERLRAAGAIVMGKTVTTEFATRTPGKTRNPHHPGHTPGGSSSGSAAAVA
                     AGMVPLALGSQTGGSIIRPGSYCGVFALKPTFGLISRHGMFRLSRSLDHVGLFARDVD
                     DLALLLEELAGFDERDPDTHPRARALYREAALEPPPRAPRFGFFRSGRWSRVSEDAQA
                     AFLALVERLGAQVEEVELAVGADELAERHRVVMDAEVAINLEREWETGRDRLSLSLRA
                     RIESGRAGRALEYLQARAALPPLHALLADLFERRCDAILTPAAEGTAPAGLESTGEPT
                     FCTLWTLLGLPAVSVPLLQGGNRLPIGVQLVGPRHGDGRLLRTARWLAESGGILGRP"
     gene            complement(7400..8092)
                     /locus_tag="VNU03_02065"
     CDS             complement(7400..8092)
                     /locus_tag="VNU03_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWP73261.1"
                     /translation="MRRALALAALLVGLLAPNADAATRWGWLGVRIRDLSEQEMEDIS
                     KRFGMREGFGAVIVEVIKETPAAAAGLRNGDLVVAFRDRPVVDTRSLQRAIGSTSVGE
                     TVRLTVLRPEEGRRPVQVKVGAMPEAMAADRVAAEYGFLVRDPEGQPELGGARPSAVP
                     SVAAVIPRSPAERAGLQVGDVLTEVAGRPVVSLDAVREALLAAGAEGPLPLIVRRDQE
                     RIAVTLEPTAAR"
BASE COUNT         1074 a         3205 c         2853 g         1042 t
ORIGIN      
        1 tcgctcttca tcgcgctccg caccgcccag cccgaccgcg agcagggccg gatcgccttc
       61 ggggagaccc atctcttcgt gggggcccgc tacgtggtct cggtccggca cgggcctcgc
      121 tctcctacgc cgacgtccgc acccgctgcg aggcgagccc gcagctcctg gccaaggggc
      181 ccggcttcgt gctgcacgcg atcatggact tcgtggtgga ccagtacttc ccgatcgtgg
      241 actcgctcga ggacgacctg gagacgctcg aggcccacat cttcggagac acggtcagcc
      301 gcgacaccac catgcgcatc tacaccctca agcgcgacct gctcgagatc aagcgcgggg
      361 tgtcgccact ggtcgacatc tgcaaccggc tgatgcgcac cgacctcgac ctgatccccg
      421 acgacgcgcg gccctacttc cgcgacgtct acgaccatgc gatccgcctg agcgagaagg
      481 tcgacgggct gcgcgagctg ctcggcaccg cgctcgaggc caacctctcg ctcaccacca
      541 tcgcccagaa cgaggccacc aagcggatca ccgggtgggc cgccatcttc gcgatcccca
      601 ccgtgatcgc cggcgtctac ggcatgaact tcgagttcat gcccgagctg cgctggcgat
      661 ggggctaccc ggtggtcatg accgtcaccg tctccctgtg cgggatcctc tactaccgct
      721 tcaagcgctc cggctggctc tgatagaatc accgcggcca tgcgcgtcct cgtgatgggc
      781 ggtaccgagt tcatcagcct gcacctgctg cgggcgctcc aggcccgcgg ccacgaggtc
      841 accgtcttca accgcggccg ccagcccgac cgcctgcccg ccggcgtcac cgtcatcgcg
      901 ggcgaccgga aggaccacgc gggcctgcgc gagcgcctgg ccggcctccg cttcgacgcg
      961 gtctacgacg tcacctacgc gccgaccctc ggcgaggacg tggccgcgct ggtcgacgcg
     1021 ctcgcgggcg cgccgcacgt gatcttcgtc tcgacctgcc gggtctacga ccacgcgctg
     1081 cccatcccct actccgagga gaccgcgcgc gggctctact ggggcgacta cgcgcggcac
     1141 aagatcggcg gcgaggacgt gctgctcgag cggcaccgcc gggacggcct gccggtgacc
     1201 atcgtgcggc cgacccacgt gatggggccg ctcaacaccc gcaacaacga aaccttcttc
     1261 atggaccgga tccatcgcgg gcgcccggtg ctggtgcccg gccacggcgg ctggctgcgc
     1321 cagttcggcc acgtcgagga tctcgccgag gtcatggccg cgctgccctg cgacgcgcgc
     1381 gcccacggcc aggcctacaa cgtcatgggc gaggacgtgg tcagccaggt cggcttcgtc
     1441 gagctggtcg cggaggcgat gggccgcccg gtgaccttcc gccacttcga tcccgcgctg
     1501 ctgaagagct tcgcgaagcc gggcccggtg ttcggccaga acctcgtcta cgactgccac
     1561 gcggtccaca ccacgaccaa gctgcgcgcg gacctgggcc tccggccccg ctacacgctc
     1621 cacgccgggc tggtgcagac ctgggagtgg tatcgcgccc aggggttggc cgaccgcccg
     1681 gtcgacttct cgttcgagga ccagatcctc tccaagatcg gcgcgtgacc cgccgcatcc
     1741 tcgtgtacca cccggagccg acggacaccc gcgcctacgc ggagctgatc cggctcccct
     1801 cgcgcggctg ggcggtcacc gcggcgctca cgcccgaggc cgcgatcgcg agcgcgcctg
     1861 aagccgaggt cctctactgc tggcggcctc cgcccgagct cctggccgcc gccacgcgcc
     1921 tccgctggat ccagtgcatg ggcgccggcg tcgagcagct cctggtgccc gaggtctctc
     1981 cccgcgtcac gatcacgcgg gtcgccggca tcttcggccc gtggatggcc gagtacgcgc
     2041 tcgggtggtg tctctgggtc acccagcgca tggaccgctt ccgggccgat cagcgcgcgc
     2101 ggcgctgggc gccggtcgac ccgattccgc tgcggggtca gaccctgtgc gtggtggggc
     2161 tgggcgacat cggcgcgcgc atcgcgcgct ccgggcgcgc cctgggcctg cgcgtgctcg
     2221 gcgtctcccg gagcggtcgc gccgtcccgc ccgtggagcg cgtgtaccgc acgggcgcgc
     2281 tccgcgccgc gctccgcctc gcggacttcg tcgtgctgac cctgccgctc agcgcggcca
     2341 cccgaggtat gctcgggccc cccgcgctcg cggccatgaa gccctcggcg tggctgatca
     2401 acgtcgcgcg ggggccgatc gtggacgagg cggccctgct cgacgcgctc cgggcccgcc
     2461 ggatcggcgg cgccatcctc gacgtcttcg acaccgagcc gctgcccccc gagcacccgc
     2521 tgtgggcgtt cgacaacgtg gtgatcaccc cgcacatctc ggggcccagc accccgcgcg
     2581 agatcgcccc gatcttcgcc gacaacctga ggcgctacct ggccggccgc cccctccgaa
     2641 acgcggtcga ccgccgccgc ggctactgat ggcgacgatc gaggcgctct gggccgcctg
     2701 ggatcagccg ggtctcgagc acctccgcct gcgggtcgac gccggcggcg cgcgggccga
     2761 tagcgtgatc atcgcgctcg acgacgacgg ccggcccttc cgggcccgct tcgcgatcga
     2821 gaccgacccg gactggcggg tgcgccacgc gcggatcgag ctgctcggcg actcggcgcg
     2881 ggccctcgac ctccacgcgg acgggctcgg ccactggacc gacgcggtca ccggcgccgc
     2941 ccagccgctc gacggctgcc tcgacatcga cgtgtacccg tcgcccttca ccaacaccct
     3001 gccgatccgg cggctgcccg cgctcgcgcc gggcgctccg gtggccatcg cggtcgccta
     3061 cgtgaccctg ccggagctga ccgtgcgccc ggcccctcag gagtacatcc tgctcgagcg
     3121 gcagcccgac ggcgcgcgat ggcggttccg cagcctcgac agcggcttca ccgcggagct
     3181 cccggtggac gcggaggggc tggtgcgcga ctacccgggg atcgcgcgcc ggctgcgcta
     3241 ggcgcgctcg atcccgaccc ggatcacctc gccccgctgc gtccagcgcg ccaggggcag
     3301 cgtgagctcg agcgccagct tgagtgcgcc atagaccggt cgccactcgg ctacccgcgc
     3361 gagccacgac gcgcggtggt cgcgcagcca gtacccgaga ctccacaggt agtaccgcgg
     3421 cttcgcctgg tgccagcacc agaccacgcg cccgcccgcc tgcgcggccg cccgctcgag
     3481 cgtggccggc gtgaagtgcg acaggtgccg cggcagctcg aggcccgacc aggcgcggcc
     3541 gaagatccgc gcgccgagcc cgcccgcgtt cggcacctcg atcacgagca ggccgcccgg
     3601 cgcgagccag ccgagcatcc gccgcaccgc gcccaccgga tcgggcacgt gctcgaggac
     3661 gtgcagcgcg gtgatcagat cgaactcgcc cgccgcgaac ggcgccgcct gcagatcacc
     3721 ggtgtgcacc cttggggtga agcggcgcgc cttcgcggcc gccgccgcgt ccacctcgac
     3781 cccggccacg tgccagccga gcgcggcggc gaccccgagc gctccgcccg agccgcatcc
     3841 cacgtcgaga tagcggcccc gcccgcgcca cgccggacag tcccagcgca ggcggcgcag
     3901 cagcaggcgc gcgcgcaacc gcgtgaggag cccggcgccc gccgcccgcc agacggtgta
     3961 gccgagctcc gaggcgagcg cccaccggac cgcgcggaca cgcgcggatc cgcccttgaa
     4021 gggaatgcgc ggcgacggct cctggtggcg cgggtagtgg tcggggtagc agtcggccag
     4081 gtgctcgtcc cgcacccgcg gccgctggta gagaaagccg cagcggtcgc agcgcgccac
     4141 cgtgtacacc ccgggcaccg agaaggcgcg atcgggggtg gcccagagcg gccgcgcgtc
     4201 ggcggcgccg cagagaaagc acggcgcctc caccgtgggg agggccgcgg cgccgaccgc
     4261 ctcacccggc gcgcggaccg gcattacttc ttcgacagga gccagtgtcc gaccgccagg
     4321 tgcggcaggc cggagcgctc gaacgtgtcg atcgcgtcgg ccgcgctccc cacgatcggc
     4381 tcgccgtgga ggttgaacga ggtgttcagc accgcgccca cgcccgtgcg ccgctcgaac
     4441 tcgcggatga ccgcgtggta ctccgggttc cactcccgct cgaggatctg cgggcgcgcc
     4501 gtcgcgtcct ggggatggag cgcggccgcc agcgcctctc gcgcctccgg ccgggtgggc
     4561 atcgccagca tcatgtaggg cgaggccagg tccttggggt tgaccaggta gtcccccgcc
     4621 cgctccgcca gcaccgtcgg cgcgaacggc atccagaagt cgcggttctt gatcatccgg
     4681 ttgatcaggc ccaccacgcg atgatccgac gggttggcca ggatcgaccg gttgccgagg
     4741 gcgcgcgcgc cgaactccat gcggccggcg caccgggcca ccaccccgtc ggagaccagc
     4801 agctcggcga tgcgctcctc gatgcgatcg tggtaggcga ccttgtactg cccctcgagc
     4861 ccgcgctcgc ggatgacctg ctcggcctcc tcgtcggtca ccgacgggcc caggtaggcg
     4921 gggccgaagg cctgcggggc cgcgggcacc ccgcgccgcg cgcactcctt caggtacccg
     4981 aggtaggccg cgcccaccgc gttggactcg tcgccgcacg acgggaacgc gaacagctcc
     5041 tcgacccact cctcctggcc gatgagcatg ttggccttca cgttcatgaa gacgccgccc
     5101 ccgagcgcga gccggccgcc gccgtagcgc tcccgcatga ggcgcgtcca ccgcagcagc
     5161 acgtcctcca ggaggcgctg ggcccccccc gcgacccagt cgaagcgcag gcccaccatc
     5221 gcgcgcagca ggagctggta gcgctctccg ggggcgcgcc accggaagcg cgcgggccgg
     5281 ccctcctcca ggtcgaagac cgcccgcagc gcctcctccg cgcgccgggc gtacttgtcc
     5341 ggggcgtagg gcgccatgcc catcaccttg tactcgtgct cgccgaactt catgccgagg
     5401 gccagcgtgg cgaacgagta gaaggcgccc agcgagccgg gggcgctcgg ggtggcctcg
     5461 tgccggtcga gggtcagccc cgagccggtg gacgcggtcg cgcagaggcc gtcccccgag
     5521 ttgtcgttgg tgagcacgag ggtcgggcgg cccgcgaagc ccgagcccca gtaggcggcg
     5581 gccgcgtggc aggtgtggtg gtcgaggcac gcgatgcggt cggcgccgat ccccaggtgc
     5641 ccggtgaccg cgccgaggcg ctcccgctgc gagatgccga acttgccgcg cgaggcgtcg
     5701 atgagcccga acttcgcacc ccacttccgg accttcttct cgaaggcccg ccgccgcgtg
     5761 ggccacgcca ctccgtagta ctcgcgggcg tagtcctcgt cgtgcagcac ccggttgagc
     5821 cactcgcgcg aggcggcgcg cgccccggcc agggccacca ggtcgatctg atcgggccgg
     5881 atgtcgagct cgcgcagcag cgcgtcgatg gcccggcgcg gatagcccgc gtcgttcttg
     5941 agccggctga accgctcctc ggaggcgcac cccacgatgc ggccgtcgcg cagcaccgcc
     6001 gcggtcgcgc agtgggtctc ggtgatgccg agcacgatca tggccggccc agtataccgc
     6061 ccgactccgc cagccaccgc gcggtacgga gcagccgccc gtccccgtgc cgcggaccca
     6121 cgagctgcac cccgatcggc aggcggttac ccccctgcag cagcggcacg ctcaccgccg
     6181 gcagccccag cagcgtccac agcgtgcaga aggtcggctc gccggtcgac tcgagcccgg
     6241 ccggggcggt gccctccgcg gcgggcgtca ggatcgcgtc gcagcggcgc tcgaacaggt
     6301 cggcgaggag cgcgtgaagc ggcggcagcg ccgcgcgcgc ctgcaggtac tcgagggccc
     6361 ggccggcccg gccgctctcg atccgcgccc gcagggagag cgacagccga tcgcgccccg
     6421 tctcccactc gcgctccagg ttgatggcga cctcggcgtc catcaccacg cggtggcgct
     6481 cggccagctc gtccgctccc accgccagct cgacctcctc gacctgcgcg ccgaggcgct
     6541 cgaccagcgc caggaaggcg gcctgagcgt cctcgctcac ccggctccag cgcccgctgc
     6601 ggaagaagcc gaagcgcggc gcgcgcggcg gcggctcgag cgcggcctcc cggtagagcg
     6661 cccgcgcgcg cggatgcgtg tcgggatccc gctcgtcgaa gccggccagc tcctcgagca
     6721 gcagcgcgag gtcgtcgacg tcgcgcgcga acagccccac gtggtcgagg ctccgcgaga
     6781 gccggaacat gccgtggcgc gagatcaggc cgaacgtggg cttgagcgcg aacaccccgc
     6841 agtaggagcc gggccggatg atggagccgc cggtctggct gccgagcgcg agcggcacca
     6901 tgcccgcggc caccgccgcc gcggagccgc tcgaggagcc gcccggcgtg tggccggggt
     6961 ggtgcgggtt acgggtcttg ccgggcgtcc gggtggcgaa ctcggtggtg accgtcttgc
     7021 ccatgacgat agcccccgcg gcccgcagcc gctccgtcac cgccgcgtcg cgggacggcg
     7081 tccggcccgc gtgcagcacc gacccgttct cggtgggcat gtccgcggtg tcgatgatgt
     7141 ccttgagccc gaccggcacc ccgtgcggcg cgccgagcgg ccgcccggag cgccgccact
     7201 cgtccgccgc gcgggcctgc gccatcgcgt ggtccgggtc gaggaaggtc cacgcctgca
     7261 cgtccggctc gacccgggtc acgcgctcga ggcaggcggc gaccagctcg cccgactcga
     7321 tcgtccccgc ccggatcagc ccggccgcct cggtcgccga cagccgccac agctccgctg
     7381 agctcatgga gcgggcggct cagcgagcgg cggtgggctc gagcgtcacc gcgatccgct
     7441 cctggtcccg gcggacgatg agcgggagcg gcccctcggc cccggccgcg agcagcgcct
     7501 cgcggaccgc gtcgaggctc accaccgggc ggcccgcgac ctccgtgagc acgtcgccca
     7561 cctggaggcc ggcccgctcg gccgggctgc gcggaatcac cgccgcgacc gacggcaccg
     7621 cgctcggccg ggcgccgccc agctcgggct ggccctccgg atcgcggacg aggaagccgt
     7681 actcggcggc cacgcggtcg gcggccatcg cctccggcat cgcgcccacc ttcacctgga
     7741 ccggccgccg gccctcctcg ggccgcagga cggtgagccg caccgtctcg ccgaccgacg
     7801 tggagccgat cgcccgctgc aggctccggg tgtcgaccac cgggcggtcg cggaaggcca
     7861 ccacgagatc gccgttgcgg aggcccgcgg ccgcggcggg cgtctccttg atcacctcca
     7921 cgatcaccgc gccgaagccc tcgcgcatgc cgaagcgctt ggagatgtcc tccatctcct
     7981 gctcggagag atcccggatg cgcacgccca gccagcccca ccgggtcgcc gcgtcggcgt
     8041 tcggggcgag gaggcccacc agcagcgcgg ccagggcgag cgcgcgtctc acgcgcgacc
     8101 tccctcgccg gtgagatggc tcagcagatc atcgagcgcc gccgcgaggc gctccagtcg
     8161 acgctccacg tgca
//
LOCUS       DATMCV010000084         5293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_537263, whole genome shotgun sequence.
ACCESSION   DATMCV010000084 DATMCV010000000
VERSION     DATMCV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537263"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VNU03_02070"
     CDS             complement(<1..625)
                     /locus_tag="VNU03_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWP73262.1"
                     /translation="MADRGIDVVRAHLAKLPPSDSLTVAERRAQYERAEKAFPTPAEV
                     KVERVSAPVAPAEWLRPPGAVAGRVVLYLHGGGYVIGSPRSHRHLAAAIATAGQAAAL
                     LLDYRLAPEHPYPAAVDDAAAAYRWLLDQGIAPGQIVIAGDSAGGGLTMAALLAPRDA
                     RLPQPAGGVCISPWVDLTCSGASYRTKAGADPIVQRTGVEEMARAYLG"
     gene            709..1545
                     /locus_tag="VNU03_02075"
     CDS             709..1545
                     /locus_tag="VNU03_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP73263.1"
                     /translation="MSPNPAPSAPAFLYGTAWKEDRTPALVELALRTGFRGIDTANQR
                     RHYFEAGVGQGLAAAYRAGVVTRDDLFLQTKFTYQPGQDHRLPYDPAAELSTQVAQSM
                     ASSLEHLDTDHVDSFVLHGPASGYGWSDYDVEVWGAMLAERAAGRTRLVGVSNVSLLH
                     LRQLAASGAAMPAFVQNRCFARRGWDRDVRSFCTERRIVYQGFSLLTANPEVVGHPLI
                     ARIAARERATPAQVVFRFALAVGMLPLTGTSDREHMAQDLASAALTLSPDEVETIESM
                     AG"
     gene            1565..3181
                     /gene="ggt"
                     /locus_tag="VNU03_02080"
     CDS             1565..3181
                     /gene="ggt"
                     /locus_tag="VNU03_02080"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWP73264.1"
                     /translation="MRYRKRAMIVAPQPEAAEAGADILRSGGNAVDAAMACALVQGVV
                     DPMMCGVAGFGSCGIVMPAAGFHGYIDFHAPAPLAVRPDMWADRIESEARDGYGFILR
                     GRVNDIGYQSICVPASLRAYRDAHREHGRLPWEQIVEPAIGWAEGGWTVRPHVGSWWA
                     EDGQMGRAGNHERLGFTAASRALYCRPDGTPKRVGDRVVNRDLGQTLRLIAKHGDRVF
                     YEGEIAHAIVEDMRRHGGLLGAADLAGWRTVRNAPLWGTYRGYRVSSNQPPGGGVMLL
                     EMLNVLERFPLGQLEHNSAEYVRIVAETMKRATIDKDRHVGDPRFVDVPVERLIGREY
                     AAGLAEDIRAGKKAQVPRLNAGAPRPDTTHVSVVDADGGCVTMTHSLGMPSGVITDGL
                     GFMYNGCMGVFDPRPGHAGSLAPGKARFSSVVPSIVFEGERPHIVIGAPGATQIAMGV
                     LQVLLNAIDFDMTMVEAVSAPRFSATSDAIDVSNRIPRAVERALTAQGYEVVRGPNTY
                     GFAAVHGIRVHPDGLDGGADPSHDGIVLAV"
     gene            3196..>5293
                     /locus_tag="VNU03_02085"
     CDS             3196..>5293
                     /locus_tag="VNU03_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWP73265.1"
                     /translation="MRAGRAGAALLAGALLLVALGSSAEAQRPGGGGKGRHRGGGAER
                     EKSAQEALGPGEYALDGAAAHEAINRGEGSQALAYYERVGEEAGRDGSQARAARAWHA
                     ATVTALRLGRFQKVIQDGGRAIEMFKAAGGLTPSDLGSWASVHAQLGSAYRAVGDLPR
                     ARATLEEGVALARTRLSGRREDQAEGYLLNGLAQVAYAQRDYPAALARSTQAAQFFEG
                     VESGLPPKGQEHLRTKLRRWNAASLAMVGRAQLALGHPDEADQAFDRGLRYARLTGLR
                     DTEADMLAGQGSLALARQDWSRALGIYQQAIVLATQVKRLGRLPALYQGQARALTGLG
                     RTEEAFAASREAVRSIEELRADLGDSSLRTGFFEDKQAIYQHAVALALQAGHPEDAFV
                     MAEQGRSRTFLDLLGSQTTLSKGRTRALVDEEVRLRARLAEARADAEDVDGSADSERA
                     RGQAETLERDYRAFLERVRKENLEQASLMAVEPVTRPEIQRLLPAGTTLLEYQVGEAG
                     AVVWVVERERFAMVRLPGDRSSLLAQVRRFRGAITDQAPLVEVEQQARALHRRLLEPA
                     RSEIHGDRLVIVPHGILHYLPFAALRSPAGRWLVEDFALSTLPSASVLRYLVDKGADA
                     SARALVVGNPDLGPALALPWAEREARMVGQHERESTVLVRADATEAQVKALLGTVGLV
                     HLATHGELSESDPLSSA"
BASE COUNT          694 a         1910 c         2027 g          662 t
ORIGIN      
        1 cgccgaggta ggcgcgcgcc atctcctcga ccccggtgcg ctgcacgatg gggtcggcgc
       61 ccgccttggt ccggtagctc gcgccgctgc aggtgaggtc cacccacggc gagatgcaga
      121 cgccgcccgc cggctggggc aggcgcgcat cgcggggggc cagcagggcc gccatcgtga
      181 gcccgccgcc cgcggagtcg cccgcgatca cgatctggcc cggggcgatg ccctggtcga
      241 gcagccagcg gtaggcggcg gccgcgtcgt cgaccgccgc cgggtacggg tgctccggcg
      301 ccaggcgata gtcgaggagc agcgcggcgg cctggccggc cgtggcgatg gccgcggcca
      361 ggtggcgatg cgagcgcggc gagccgatca cgtagccgcc cccgtggagg tacaggacca
      421 cgcgcccggc caccgcgccc gggggccgca gccattccgc gggcgcgacc ggggcgctca
      481 cgcgctccac cttgacctcg gccggggtcg ggaaggcctt ctcggcgcgc tcgtactggg
      541 cgcggcgctc ggccaccgtg agcgagtcgg acggggggag ctttgccagg tgcgcgcgca
      601 cgacgtcgat gccgcggtcg gccatgtcga tcctcctgga cgctgagctt agtcgcgacc
      661 gatctgcgat acaagaccgc cgggcggtcg ccgtggcaga atggcgcgat gtcaccgaac
      721 cccgccccgt ccgcgcccgc cttcctctac gggaccgcct ggaaggagga ccgcaccccg
      781 gccctggtcg agctggccct gcggaccggc ttccgcggca tcgacaccgc gaaccagcgg
      841 cggcactact tcgaggccgg cgtcggccag gggctcgccg cggcctaccg ggcgggggtg
      901 gtcacgcggg acgacctgtt cctccagacc aagttcacct accagccggg gcaggaccac
      961 cggctgccct acgacccggc cgccgagctc tccacccagg tcgcccagtc gatggccagc
     1021 tcgctcgagc acctggacac cgaccacgtg gacagcttcg tcctgcacgg ccccgcctcc
     1081 gggtacggct ggagcgacta cgacgtcgag gtgtggggcg ccatgctggc cgagcgcgcc
     1141 gccggccgca cccgcctcgt gggcgtgagc aacgtgtcgc tgctccacct gcgccagctc
     1201 gcggccagcg gcgcagcgat gccggccttc gtgcagaacc gctgcttcgc ccgccgcggc
     1261 tgggaccgcg acgtccgctc gttctgcacg gagcgccgca tcgtctacca gggcttctcg
     1321 ctgctgaccg cgaacccgga ggtggtcggc catcccctga tcgcgcgcat cgcggcccgg
     1381 gagcgggcca ccccggcgca ggtggtgttc cgcttcgccc tcgcggtggg gatgctgccg
     1441 ctgaccggga cctccgaccg cgagcacatg gcgcaggacc tggcgagcgc ggcgctgacg
     1501 ctctctcccg acgaggtgga gacgatcgag tcgatggccg gttgacccga ggggaggacc
     1561 gacgatgcga taccgcaagc gagcgatgat cgtggccccg cagccggaag cggccgaggc
     1621 cggcgccgac attttgagat cgggcggcaa cgcggtggac gccgcgatgg cctgcgcgct
     1681 ggtgcagggc gtggtggacc cgatgatgtg cggcgtggcc ggcttcggca gctgcggcat
     1741 cgtgatgccc gcggccggct tccacggcta catcgacttc cacgcccccg cgccgctcgc
     1801 ggtccggccc gacatgtggg ccgatcgcat cgagtcggag gcgcgcgacg gctacggctt
     1861 catcctgcgc ggacgcgtga acgacatcgg ctaccagtcg atctgcgtgc cggcgagcct
     1921 gcgggcctac cgcgacgcgc accgggagca cggccggctg ccgtgggagc agatcgtgga
     1981 gcccgccatc ggctgggccg agggtggctg gacggtgcgc ccgcacgtgg gctcgtggtg
     2041 ggcggaggac gggcagatgg gccgggccgg caatcacgag cggctcggat tcaccgcggc
     2101 ctcgcgcgcg ctctactgcc ggcccgacgg cacgcccaag cgggtgggcg atcgcgtggt
     2161 caaccgcgac ctgggccaga ccctgcgcct gatcgccaag cacggtgacc gggtcttcta
     2221 cgagggcgag atcgcgcacg ccatcgtgga ggacatgcgt cggcacggag gcctcctcgg
     2281 ggcggcggat ctcgccgggt ggcgcaccgt ccgcaacgcg ccgctctggg gcacctaccg
     2341 cggctaccgc gtgtcgagca accagccgcc cggaggcggg gtgatgctgc tcgagatgct
     2401 gaacgtcctg gagcgcttcc cgctgggcca gctcgagcac aactcggccg agtacgtgcg
     2461 gatcgtggcg gagaccatga agcgcgcgac catcgacaag gaccggcacg tcggcgatcc
     2521 gcgcttcgtc gacgtaccgg tggagcggct catcggccgg gagtacgcgg ccgggctcgc
     2581 ggaggacatc cgcgccggca agaaggcgca ggtgccgcgg ctcaacgcgg gcgcgccgcg
     2641 gccggacacc acccacgtct cggtggtcga cgccgacggg ggctgcgtga ccatgacgca
     2701 ctcgctcggc atgccctccg gcgtgatcac cgacgggctc ggcttcatgt acaacggctg
     2761 catgggcgtc ttcgacccgc ggccgggcca cgcgggcagc ctggcccccg gcaaggcccg
     2821 cttcagctcg gtggtcccgt ccatcgtgtt cgagggcgag cggccgcaca tcgtcatcgg
     2881 ggcgcccggg gcgacccaga tcgcgatggg cgtgctccag gtgctgctga acgccatcga
     2941 cttcgacatg acgatggtgg aggcggtcag cgcgccgcgg ttctcggcga cgagcgacgc
     3001 gatcgacgtc tccaatcgca tcccgcgcgc ggtggagcgc gcgctcaccg cgcagggcta
     3061 cgaggtggtg cgcggcccca acacctacgg cttcgcggcg gtccacggca tccgggtgca
     3121 tccggacggg ctcgacgggg gcgccgaccc gagccacgac ggcatcgtgc tggcggtgtg
     3181 atcggcggct ctcccgtgcg cgccggccgg gccggggccg ctctgctggc cggcgcgctg
     3241 ctgctcgtcg cgctgggctc gtccgcggaa gcgcagcgcc cgggcggcgg cggcaagggg
     3301 cgccaccgtg gcggcggagc ggagcgcgag aagagcgccc aggaggcgct gggccccggc
     3361 gagtacgcgc tcgacggggc ggcggcgcac gaggccatca accgtggcga ggggagccag
     3421 gcgctcgcgt actacgagcg cgtcggcgag gaggccgggc gggacggcag ccaggcgcgc
     3481 gcggcccggg cgtggcacgc ggcgacggtg accgcgctga ggctcgggcg cttccagaag
     3541 gtcatccagg acgggggccg cgcgatcgag atgttcaagg cggccggtgg gctgacgccg
     3601 tccgatctcg gctcgtgggc gtcggtgcac gcgcagctcg gctcggcgta ccgggcggtc
     3661 ggcgacctgc cccgggcccg cgcgacgctc gaggaagggg tcgcgctcgc ccggacccgg
     3721 ctgagcgggc gacgggagga ccaggccgag ggatacctgc tcaacggtct cgcccaggtg
     3781 gcctacgccc agcgggacta cccggccgcg ctcgcacgca gcacccaggc cgcccagttc
     3841 ttcgagggag tcgagagcgg gctgcccccg aagggccagg agcacctgcg cacgaagctc
     3901 cgccgctgga acgcggcctc gctcgcgatg gtcggtcgcg cccagctcgc cctcggccat
     3961 cccgacgagg cggaccaggc cttcgatcgt gggctgcggt acgcgcggct gaccgggctg
     4021 cgcgacaccg aggccgacat gctcgcgggt cagggaagcc tcgccctcgc gcggcaagac
     4081 tggtcgagag cgctcggcat ctaccagcag gcgatcgtgc tcgcgacgca ggtcaagcgc
     4141 ctgggccgcc tgcccgcgct ctaccagggc caggcgcgcg cgctgaccgg gctgggccgg
     4201 acggaagagg ccttcgcggc ctcgcgtgag gcggtgcgct ccatcgagga gctgcgcgcg
     4261 gacctgggcg actcgagtct ccggaccggg ttcttcgagg acaagcaggc catctaccag
     4321 cacgcggtgg cgctcgcgct ccaggcgggg caccccgagg acgccttcgt gatggccgag
     4381 cagggccggt cgcgcacctt tctcgacctg ctcgggagcc agaccacgct ctccaagggc
     4441 cgcacccgcg ccctggtcga cgaggaggtg cggctgcggg cccgcctcgc cgaggcccgc
     4501 gccgacgcgg aggacgtgga cggatcggcg gactcggagc gcgcccgcgg ccaggccgag
     4561 acgctcgagc gcgactaccg cgcgttcctc gagcgggtgc gcaaggagaa cctggagcag
     4621 gcctccctga tggcggtcga gccggtgacg cggcccgaga tccagcgcct gctgcccgcg
     4681 ggcacgacgc tgctcgagta ccaggtgggc gaggccgggg cggtggtgtg ggtcgtggag
     4741 cgcgagcggt tcgcgatggt gcggctgccc ggcgaccggt cctcgctcct cgcgcaggtg
     4801 cggcgcttcc gcggggcgat cacggaccag gccccgctcg tcgaggtcga gcagcaggcc
     4861 cgcgcgcttc accggcgtct gctcgagccg gcgcggtcgg agatccacgg cgaccggctg
     4921 gtgatcgtgc cgcacgggat cctgcactac ctgccgttcg cggcgctccg ctcgccggcc
     4981 ggccggtggc tcgtggagga cttcgcgctg agcacgctgc cgagcgcgag cgtgctgcgc
     5041 tacctggtcg acaagggtgc cgacgcctcc gcccgggccc tggtggtcgg caacccggac
     5101 ctgggcccgg ccctcgccct gccgtgggcc gagcgggagg cccggatggt cggccagcac
     5161 gagcgggaat cgacggtgct ggtccgcgcc gacgcgaccg aagcccaggt caaggcgctg
     5221 ctcgggacgg tggggctcgt ccacctcgcc acccacggcg agctgagcga gtccgacccg
     5281 ctctcctcgg cga
//
LOCUS       DATMCV010000085         5101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_4934, whole genome shotgun sequence.
ACCESSION   DATMCV010000085 DATMCV010000000
VERSION     DATMCV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4934"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VNU03_02090"
     CDS             complement(<1..205)
                     /locus_tag="VNU03_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73266.1"
                     /translation="MRPLGRRTGFHTLDRWLYNAGVVLRPPRDTDLVLGVDLDGFLWA
                     RRRALPFVASLKGIIADELKNERG"
     gene            complement(323..1108)
                     /locus_tag="VNU03_02095"
     CDS             complement(323..1108)
                     /locus_tag="VNU03_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWP73267.1"
                     /translation="MSDHILVEQDGPIATVVFNRPKMRNAISLAMWSEIAMVTERLSK
                     DDAVRAIVYRGAGTDAFASGADISEFQENRKDTATALNYNKQTEAAYSSIRLCPKPTV
                     AMVFGYCMGGAMALAMAADFRFAAGGSKFGIPAARLSIIYGLDPVHQLVDLVGPAYAK
                     DILYSARTVDAEEALRIGFIQRLVPAAELETYTYDYLKKVAANAPLSVRGTKAQVQAI
                     FEGISDAHREHLRDLGIATFDSEDYREGTRAFLEKRAPRFQGR"
     gene            complement(1105..2304)
                     /locus_tag="VNU03_02100"
     CDS             complement(1105..2304)
                     /locus_tag="VNU03_02100"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP73268.1"
                     /translation="MTPTTASRPLARFSVLDLTTVRSGPTCTKILADFGADVVRVERP
                     GGEGRERVFFDAADLHRNKRSIAVNLQEPRGVAVVKRLAARSDVVVENYRPDVKHRLG
                     VDYEALSRENPRLVYASISGFGQEGPYRDRPGYDQIVQGMSGLMWLTGTAESAPLRVG
                     IPIGDLLAGYFCAIGILTALLEREVSGRGQKVETSLLEALTGSLSFQAAKYVNTGEVP
                     PPVGNHHPLTAPMGVYRAQDGFFNLAVGNDDMWRRFCKAIDQPALADDARFAGMLARV
                     RNRPALDVVLEAAVARRPAAEWVESLNAAGVACGPINTVDRVFADPQIRQADLVAEVA
                     NATWGPHKVLALPVHLSRTPARVEHAAPMTGEHTREILTALGYDAAALDALLADGVIE
                     QYKGETT"
     gene            2423..2566
                     /locus_tag="VNU03_02105"
     CDS             2423..2566
                     /locus_tag="VNU03_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73269.1"
                     /translation="MTLALFGVAAVVFSILLGVVHTVSERRQQRRIQQQWEARERSLN
                     RKP"
     gene            complement(2587..2662)
                     /locus_tag="VNU03_02110"
     tRNA            complement(2587..2662)
                     /locus_tag="VNU03_02110"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2627..2629),aa:Met,seq:cat)
     gene            complement(2750..3163)
                     /locus_tag="VNU03_02115"
     CDS             complement(2750..3163)
                     /locus_tag="VNU03_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73270.1"
                     /translation="MSPDPATRRRILVVDDDERERAGAAQALGEAGYVVDSAATKAEV
                     VTLLAKHNYDLVLADLKMPGINGPAFYQMLKEACRGTTPPVIFSLQRGYTPEYANFLM
                     RLAAPVLMKPLPAAELRQAVERLLPTPRATSSLAS"
     gene            complement(3160..3459)
                     /locus_tag="VNU03_02120"
     CDS             complement(3160..3459)
                     /locus_tag="VNU03_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73271.1"
                     /translation="MSEEIVRWSDEGRQLLQTLGGWLEAAGSIHDEAEVLERERAALA
                     AEVRRLEEDNDALRSQRDELHEAVQTLAGQMTRTADEILYQFGARRGSPGDLASR"
     gene            complement(3456..3812)
                     /locus_tag="VNU03_02125"
     CDS             complement(3456..3812)
                     /locus_tag="VNU03_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HWP73272.1"
                     /translation="MPLFAVERDLSQMPPERFRTELKSLVSACARLKGAGKQVRYISS
                     AVFPAEARGLCLFGAEQPQWIHEANEAAGLPYSRIFAVLDLTPSGVRRDLSCGRRPAE
                     GSDGGTAAAGPARRVR"
     gene            4163..4414
                     /locus_tag="VNU03_02130"
     CDS             4163..4414
                     /locus_tag="VNU03_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73273.1"
                     /translation="MIRLGNRPWIEEDEQTPIGLADEDDKDEDEEDKEDDDDLDEDDD
                     VDDEDDDFDDDDDFEDDDLDDDDDDDEAEVVEDDAEDEG"
     gene            4454..>5101
                     /locus_tag="VNU03_02135"
     CDS             4454..>5101
                     /locus_tag="VNU03_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HWP73274.1"
                     /translation="MALMSTEGWLFLLRWIHFLAGITWIGLLYYLNFAQVPFFAETEP
                     GVRSGAQQKLLPRVMWWFRWGAMITFLSGWLYLLHYWIGIRGIQDPGTWKILLGALLG
                     SIMWANVWFVIWPKNKIVIQNALDTAAGKPANPAAAAAGARGGLASRTNVVLSVPMLF
                     FMGSASHLAGLTVGKSGLLFWIVALAIMVLVEINALVGTPGKGAAKPLATVKGTLW"
BASE COUNT          767 a         1736 c         1814 g          784 t
ORIGIN      
        1 ggccgcgctc gttcttgagc tcgtcggcga tgatgccctt gaggctggcc acgaacggca
       61 gcgcgcgccg ccgcgcccag aggaagccgt cgaggtcgac ccccagcacc aggtcggtgt
      121 cccggggcgg gcgcagcacc acgcccgcgt tgtagagcca gcggtcgagg gtgtggaacc
      181 ccgtccgccg gccgagcggg cgcaccgtga cctggtggcc gagctgggtc agcccctggg
      241 ccagccccgc gatgccgacg aaggtgccac tcccctccag cggattgagg ggagtcgagg
      301 agagaacggt gacccgcaga ggctatcgcc cctggaaccg gggcgcccgc ttctcgagga
      361 aggcccgcgt gccctcccgg tagtcctcgc tgtcgaaggt ggcgatgccc aggtcgcgca
      421 ggtgctcccg gtgcgcgtcg ctgatgccct cgaagatcgc ctggacctga gccttcgtgc
      481 cgcggaccga gagcggcgcg ttggccgcga ccttcttgag gtagtcgtag gtgtacgtct
      541 cgagctccgc ggccgggacc agccgctgga tgaagccgat gcgcagggcc tcctccgcgt
      601 ccaccgtccg cgccgaatac aggatgtcct tggcgtaggc gggcccgacc agatcgacga
      661 gctggtgcac cgggtcgagg ccgtagatga tgctgagccg cgcggccgga atgccgaact
      721 tggagccccc cgccgcgaag cggaaatcgg ccgccatcgc cagcgccatg gcgccgccca
      781 tgcagtagcc gaacaccatc gcgaccgtcg gcttcgggca gagccggatc gaggagtagg
      841 cggcctcggt ctgcttgttg tagttgagcg cggtggcggt gtccttgcgg ttctcctgga
      901 actcggagat gtccgccccg gacgcgaagg cgtcggtgcc cgctccgcgg tagacgatcg
      961 cgcgcaccgc gtcgtccttc gacaggcgct cggtcaccat cgcgatctcc gaccacatcg
     1021 cgaggctgat ggcgttgcgc atcttgggcc ggttgaacac gacggtcgcg atcggaccgt
     1081 cctgctcgac caggatgtgg tcgctcatgt cgtctctccc ttgtattgct cgatcacgcc
     1141 gtcggccagc agggcgtcga gggccgccgc gtcgtagccc agagcggtga ggatctcgcg
     1201 ggtgtgctcc ccggtcatgg gggccgcgtg ctcgacgcgc gcgggcgtgc gcgagaggtg
     1261 caccggcagg gccagcacct tatgcggccc ccaggtcgcg ttggccacct cggccaccag
     1321 atcggcctgt cggatctgcg ggtcggcgaa gacccggtcc accgtgttga tggggccgca
     1381 ggccacgccg gccgcgttga ggctctcgac ccactccgcg gccggccggc gggcgacggc
     1441 ggcctcgagc accacgtcga gggccggccg gttcctcacc cgggcgagca tgcccgcgaa
     1501 gcgcgcgtcg tcggccaggg ccggctgatc gatcgccttg cagaagcgcc gccacatgtc
     1561 gtcgttgccg acggccaggt tgaagaagcc gtcctgggcc cggtagacgc ccatcggcgc
     1621 ggtcagcgga tgatggttgc cgacgggcgg cggcacctcg ccggtgttga cgtacttcgc
     1681 ggcctggaac gacaggctcc cggtgagcgc ctcgagcagc gaggtctcga ccttctggcc
     1741 gcgcccggag acctcccgct ccagcagggc ggtgaggatg ccgatcgcgc agaagtagcc
     1801 cgcgagcagg tcgccgatcg ggatgcccac gcgcagcggg gcgctctccg cggtgccggt
     1861 gagccacatg agcccggaca tgccctggac gatctggtcg tagccggggc ggtcgcggta
     1921 cgggccctcc tggccgaagc cggagatgct ggcgtacacg agccgcgggt tctcgcgcga
     1981 cagcgcctcg tagtccaccc ccaggcggtg cttgacgtcg gggcggtagt tctcgaccac
     2041 cacgtccgac cgcgcggcca ggcgcttgac gacggctacc ccccgcggct cctgcagatt
     2101 gaccgcgatg ctgcgcttgt tgcggtgcag gtcggcggcg tcgaagaaga cccgctcgcg
     2161 cccctccccg cccggccgct ccacccggac cacgtcggcc ccgaagtcgg ccaggatctt
     2221 ggtgcaggtc gggccggagc gtacggtggt caggtcgagg accgagaacc gcgcgagcgg
     2281 tcggctggcg gtcgtggggg tcacgaggag gtagtctagc ggacggccgg ggcgacttca
     2341 accgcctccg ggcttatttg gcaatgactt accccctgcg gtaggatcga atcatgatca
     2401 cctcgacccg acgctcccgc ccatgacgct tgccctcttc ggagtcgcgg cggtggtgtt
     2461 cagcatcctg ctgggcgtcg tccacaccgt ctccgagcgc cggcagcagc gccgcatcca
     2521 gcagcagtgg gaagcgcggg agcgctcgct caaccgcaag ccgtagccgc cgcccgccga
     2581 gggatgtggt ggcggcggga ggactcgaac ctccgacacg gggcttatga gacccctgct
     2641 ctgccacctg agctacgccg ccttggtagg aacttcgcgc agttgcagag cggaatacta
     2701 acacgcccga tcgcgggggc gcgccgaatt gtggccggcg cgagaggcgt caggacgcca
     2761 gcgaggacgt ggcgcgcggg gtcgggagca gccgctccac cgcctggcgg agctcggcgg
     2821 cgggcagcgg cttcatcagg acgggcgcgg cgagacgcat gaggaagttc gcgtactccg
     2881 gcgtgtagcc gcgctggagc gagaagatca ccggcggcgt ggtgccgcgg cacgcttcct
     2941 tcagcatctg atagaacgcg ggaccgttga tgccgggcat cttgagatcg gccagcacga
     3001 ggtcgtagtt gtgcttggcg agcagcgtga ccacctccgc cttggtggcc gcggagtcga
     3061 ccacgtaccc ggcctccccg agcgcctgag ccgcgccggc gcgctcccgc tcgtcgtcgt
     3121 cgaccaccag gatgcggcga cgggtggccg gatccgggct catcgggacg ccaggtcgcc
     3181 cggcgatccg cgccgggctc cgaactgata gaggatctcg tccgcggtgc gcgtcatctg
     3241 gcccgcgagc gtctggaccg cttcgtggag ctcgtcacgc tgggagcgaa gcgcatcgtt
     3301 gtcctcctcg agacgtcgca cctcggccgc gagcgcggcc cgctcgcgct cgagcacctc
     3361 cgcctcgtcg tggatggagc cggccgcttc gagccacccg cccagcgtct gcaagagctg
     3421 ccggccctcg tcggaccagc ggacgatctc ctcgctcacc ggactcgcct ggccgggccg
     3481 gccgccgcgg tgcccccgtc ggagccctcc gcgggtcggc gaccgcacga gaggtcgcgc
     3541 cgtactccgc tcggggtgag gtccagcacc gcgaagatcc gcgagtaggg caggccggcc
     3601 gcctcgttcg cctcgtggat ccactgcggc tgctccgcgc cgaacagaca cagcccccgg
     3661 gcctccgccg ggaacaccgc gctgctgatg tagcgaacct gcttcccggc tcccttcaac
     3721 cgcgcgcacg cgctgacgag actcttcagc tcggtccgga agcgctccgg cggcatttga
     3781 ctcaggtcac gctcgacggc gaacagcggc atgcgttgat ctcctcgtga cgccacacgg
     3841 cgtcccgcgc atcgtaggcg tcgcggagcg acgtcgtcaa gcgcccgcgc gagccgtgga
     3901 aatgcgggag gactcgctcg cgcgcgcgaa tcgccgcgtt ggatcaggcg accgcgcgcg
     3961 tcgacgcgtc ggcgccgcgc caggcttgct cgttcgtgat gcgacgcccg cgggcccgca
     4021 tcagggcgtg caattctcgt gttgtggacg gctggggata agtggataag tggctccgag
     4081 aggccaaatt ggcgtggaca cccaagaccc ctttgttata atcccgccca gcgtaacgag
     4141 ctttacgtcg ggaggatgtc gcatgattcg cctcgggaat cgcccctgga tcgaagaaga
     4201 cgagcagacg ccgatcggcc tggccgacga ggacgacaag gacgaggacg aagaggacaa
     4261 ggaggacgac gacgacctcg acgaggacga tgacgtcgac gacgaggacg acgacttcga
     4321 cgacgacgac gacttcgagg acgacgacct agacgatgac gatgacgacg acgaggcgga
     4381 agtcgtcgaa gacgacgctg aggacgaggg ctagccgcag ttcctgaccc gcaggcctcc
     4441 cgggagggtg acgatggcgc tgatgagcac cgaaggatgg ctgttcctct tgcgatggat
     4501 ccacttcctg gcgggtatca cctggatcgg cctgctctac tacctcaatt tcgcccaggt
     4561 cccgttcttc gccgagaccg agccgggggt gcgctcggga gctcagcaga agctgctacc
     4621 gcgcgtgatg tggtggttcc ggtggggcgc catgatcacg ttcctatccg gctggcttta
     4681 tcttctgcac tactggatcg ggatccgcgg gatccaggac ccgggcacct ggaagatcct
     4741 gctgggcgcc ctcctcgggt cgatcatgtg ggccaacgtg tggttcgtga tctggccaaa
     4801 gaacaagatc gtgatccaga acgcgctcga caccgcggcc gggaagccgg ccaatccggc
     4861 ggccgccgcg gccggcgcgc gcggggggct cgcttcccgc accaacgtgg tgctgtccgt
     4921 cccgatgctg ttcttcatgg gatcggcgag ccacctggcc gggctgacgg tggggaagtc
     4981 cggcctcctc ttctggatcg tggcgctcgc gatcatggtg ctggtggaga tcaacgcgct
     5041 ggtgggcacc ccgggcaagg gcgcggcgaa gccgctcgcc accgtcaagg gcaccctttg
     5101 g
//
LOCUS       DATMCV010000086         2640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_737834, whole genome shotgun sequence.
ACCESSION   DATMCV010000086 DATMCV010000000
VERSION     DATMCV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737834"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VNU03_02140"
     CDS             <1..268
                     /locus_tag="VNU03_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HWP73275.1"
                     /translation="YTSSQSLAFDEARNAGVRAARRQILVDHTRGEAGDRLRWDEVAG
                     WAERRGEVIVVAHGHPLTVRLLKEYVPRWEARGLRLVPVSTLAR"
     gene            279..1280
                     /gene="tsaD"
                     /locus_tag="VNU03_02145"
     CDS             279..1280
                     /gene="tsaD"
                     /locus_tag="VNU03_02145"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWP73276.1"
                     /translation="MIVLGIETSCDETAAAVVDGGRKVLASIVASQDDVHAPYGGVVP
                     ELASRRHLEVLVPVIRRTLETAGMGLADVDGFAVTHGPGLVGSLLIGCSAAKAIAYAR
                     RRPLVGVNHLEGHIYAAFLEGEPPAHPFLALVVSGGHTALYLAREPRRYECIGQTRDD
                     AAGEAFDKVAKLLGLGFPGGPAVERIARTGDPRAIAFPTANMSDGAPGFSFSGIKTAV
                     SLHVRRAGPLDPAQVADVAASFQATAVKMLVRETTKAARRAGVRRIVLTGGVAANTAL
                     REALEGECRERGWALHVPSRRLCTDNAAMIAAAGHDRLEAGERAPLNLNAVPDLALA"
     gene            1305..2384
                     /locus_tag="VNU03_02150"
     CDS             1305..2384
                     /locus_tag="VNU03_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP73277.1"
                     /translation="MARLDYESLLAGLPDAVVGVDDGLRVMLWNPAAESLLGRSARRT
                     VGRALKEIFPPETSLVRHLGDTLATGESRSESEAVIESGDGRPVHVSIVTAPLARSGD
                     VEAAVAVIRDMTRLHQLESEVRRGETLAAAGQIAMGLAHEIRNPLGAIRGAVQLMRRE
                     LGDDPRLGEYTDVLFKEVDRVNRIIEMLLDIGRPVTLRPVPLNVHQLLERVALLAEEM
                     ASQRGVQILRRYDPSLPPILADEDRILQVFHNLVRNAVEAMDRGGRLTLVTRLSMNPL
                     FAKVDLGHGQRSMAEIQVMDDGQGIPEATRSRLFTPFFTTKDKGLGLGLALCHRIIDE
                     HRGAIQIASEPGKGTTVSCYLPIAR"
     gene            2381..>2640
                     /locus_tag="VNU03_02155"
     CDS             2381..>2640
                     /locus_tag="VNU03_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73278.1"
                     /translation="MSDPRPTGQILIADDEDSLRWVLEKGFRGAGYQVTAVKDGTAAL
                     KEVEAAPFDLILLDVRMPGIDGLSLLKQVRDKRPDAQVVIMTA"
BASE COUNT          361 a          967 c          961 g          351 t
ORIGIN      
        1 ctacacgtcg agccagtcgc tggccttcga cgaggcgcgc aacgcggggg tgcgcgcggc
       61 gcgccggcag atcctggtgg atcacacccg tggggaggcc ggggaccggc tacgatggga
      121 cgaggtggcg ggatgggcgg agcggcgggg cgaggtcatc gtggtcgccc acgggcatcc
      181 gctgacggtg aggctcttga aggagtacgt gccgcgatgg gaagcgcgcg ggctgcggct
      241 ggtgccggta tcgaccctcg cgcgctaggc gctccccgat gatcgtgctc ggcatcgaga
      301 cctcgtgtga cgagaccgcc gccgcggtgg tggacggcgg ccggaaggtg ctcgccagca
      361 tcgtcgcctc gcaggacgac gtacacgcgc cctacggcgg cgtggtgccc gagctggcct
      421 cgcggcggca cctggaggtc ctggtaccgg tgatacggcg cacgctggag acggccggga
      481 tggggctcgc cgacgtggac ggcttcgcgg tgacccacgg acccgggctc gtcggctcgc
      541 tcctcatcgg ctgctcggcg gccaaggcga tcgcctacgc ccgccgacgg cctctcgtgg
      601 gcgtcaatca cctcgagggc cacatctacg cggccttcct ggagggcgag cctcccgccc
      661 acccgttcct cgccctggtg gtctcgggcg gccacaccgc gctctacctg gcgcgcgagc
      721 cgcgccgcta cgagtgcatc gggcagaccc gcgacgacgc ggcgggtgag gccttcgaca
      781 aggtcgccaa gctgctcggc ctgggctttc cgggcggccc cgcggtcgag cggatcgcgc
      841 ggaccggcga tccgcgcgcg atcgcgttcc ccaccgcgaa catgagcgac ggggcgcccg
      901 gcttctcctt cagcggcatc aagaccgcgg tctcgctgca cgtgcgccgc gccggcccgc
      961 tggatcccgc gcaggtggcc gacgtggccg cgtccttcca ggccaccgcg gtcaagatgc
     1021 tggtgcgcga gaccacgaag gcggcgcgcc gcgccggggt gcgccgcatc gtgctcaccg
     1081 gcggggtggc cgccaacacc gccctgcggg aggcgctcga gggcgagtgc cgcgagcgcg
     1141 gatgggcgct gcacgtgccc tcgcgccgcc tgtgcaccga caacgcggcc atgatcgcgg
     1201 cggccggcca cgaccgcctc gaggcgggcg agcgcgcccc gctcaacctc aacgcggttc
     1261 ccgacctggc gctggcatga gcgccgggct ggcctgagcg tccggtggcc cgcctcgact
     1321 acgagagcct cctcgccggc ctgcccgacg cggtggtcgg cgtcgacgac ggtctccggg
     1381 tgatgctctg gaaccccgcc gcggagtcgc tgctggggcg ctcggcccgc cgcacggtcg
     1441 gccgcgcgct caaggagatc ttcccgcccg agacgtccct ggtgcgccac ctgggcgaca
     1501 cgctcgccac cggcgagagc cggtcggagt cggaggcggt gatcgagagc ggcgacggcc
     1561 ggccggtgca cgtcagcatc gtgaccgccc cgctcgcgcg gagcggcgac gtcgaggccg
     1621 cggtggcggt gatccgcgac atgacccgcc tgcaccagct cgagtccgag gtgcgccgcg
     1681 gggagacgct ggccgcggcc gggcagatcg cgatgggcct ggctcacgag atccgcaacc
     1741 cgctgggggc gatccgcggg gcggtgcagc tcatgcggcg cgagctgggc gacgacccgc
     1801 gcctcggcga gtacaccgac gtgctgttca aggaggtcga ccgggtcaac cgcatcatcg
     1861 agatgctgct cgacatcggc cgcccggtga cgctgcgccc ggtcccgctg aacgtccacc
     1921 agctgctcga gcgcgtggcc ctcctcgccg aggagatggc gagccagcgc ggcgtgcaga
     1981 tcctccggcg ctacgatccg agcctgccgc ccatcctggc cgacgaggac cggatcctcc
     2041 aggtcttcca caacctcgtg cgcaacgcgg tcgaggcgat ggaccggggc gggcggctca
     2101 ccctcgtcac ccggctcagc atgaacccgc tcttcgccaa ggtggatctg ggccacgggc
     2161 agcgcagcat ggccgagatc caggtgatgg acgacggcca gggcatcccg gaggcgaccc
     2221 ggtcccggct cttcaccccg ttcttcacca ccaaggacaa ggggctgggc ctcgggctcg
     2281 cgctctgcca ccgcatcatc gacgagcacc gcggcgccat ccagatcgcg agcgagcccg
     2341 gcaagggcac caccgtctct tgctatctcc cgatcgcccg atgagcgatc cgcgtccgac
     2401 cggccagatc ctgatcgcgg acgacgagga cagcctgcgc tgggttctcg agaaggggtt
     2461 ccggggcgcg ggctaccagg tcaccgcggt caaggacggc accgcggccc tcaaggaggt
     2521 cgaggccgct cccttcgacc tcatcctcct cgatgtccgc atgcccggca tcgacggcct
     2581 ctcgctcctc aagcaggtgc gggacaagcg ccccgacgcc caggtcgtca tcatgaccgc
//
LOCUS       DATMCV010000087         3829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_405417, whole genome shotgun sequence.
ACCESSION   DATMCV010000087 DATMCV010000000
VERSION     DATMCV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405417"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1376
                     /locus_tag="VNU03_02160"
     CDS             42..1376
                     /locus_tag="VNU03_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP73279.1"
                     /translation="MDPRDDRRQFLRTGLAAGTMVGAAGLEGILAARRGPAFAQTTKI
                     HLLQWVDFIPEGDVEVRRQVADYTKQTKVEVTFETINANDLQARITAAIQSGAGPDLI
                     LMLHNWPHLYANALVDVSDLSDWKTKDQGGYYSHCEVATKVGSQRLALPYGTGGILTA
                     YRKSWFAEVGALQPPKTLDEYRKIGTALKKKGRPIGQTLGHTFGDAPAWTYPLLWTFG
                     GAETDASGRKVVINSKGAIEAVKWMTAFWKDACDEGALAWDDTNNNRAFHAGEISATN
                     NGASIYIFAKRNPDKIKDERGEPMWRDIQHFAIPAGPNGTTPLYLVSFAHGLMKYSKN
                     QTAAREFLKWFHSKDQFGKWFEIENGYSAGATTYWETHPMWDKVDEALKAFRLAPRGS
                     RMFGYAGPPSAKAAEAYSKYIITDMYAKAVQGMSAEDAVKWAEVELKKVYEG"
     gene            complement(1425..3008)
                     /locus_tag="VNU03_02165"
     CDS             complement(1425..3008)
                     /locus_tag="VNU03_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HWP73280.1"
                     /translation="MISTSPPVTIPNVSITDYVLRHAARLGDKPALIDGPTGRTLSYR
                     QLAEGARRTAAGLAQRGFGKGQVFAIYCPNLPEYAVVFLGVAMAGGINTTVNPLYTAD
                     ELATQLNDSGARFLVTVPAFLEKAKAAAAKSKVEEVFVFGAAEGARSFTDLLRAGDTP
                     RAVPIDPAADLVALPYSSGTTGLPKGVMLTHRNLVANLCQAEGMKNFECFDESDITVA
                     VLPFFHIYGMVVIMMLGLAGGGTILVMPRFDMMEFLALVQKYRVTILPLVPPIVLGLV
                     KHPAVAQADLSSVRLVFSGAAPLGEEMARALSARLGCPVVQGYGMTEASPVTHLSPTH
                     DAPFKPGSAGCIVPNTEVKIVDAAGASDAELPVGKEGELWIRGPQIMKGYLNRPGETA
                     ESIDRDGWYHTGDVGYVDDEGFFFIVDRTKELIKYKGLQVAPAELEALLLTHPAILDA
                     AVVRKADEEAGEVPKAYVVLKPDDASKATPADAIMGWVAERVAPHKRIRDLEFIDQIP
                     KSASGKILRRVLIDREKAR"
     gene            complement(3043..>3829)
                     /locus_tag="VNU03_02170"
     CDS             complement(3043..>3829)
                     /locus_tag="VNU03_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP73281.1"
                     /translation="ACRADAPVWIDADETRIEQILGNLLGNALKFTPADGRVTVAVTV
                     EGAEAVLRVEDTGAGIHPDLLPRIFDLFVQGQTTLHRREAGLGIGLTLVKRLVDLHRG
                     RIEAASGGPGRGSAFTIRLPAARPPGDTSALVPEMPRAAIRRRVLIIEDNDDARQMLR
                     HLLDHAGHEVYEAAEGADGLARALALSPDAVIVDIGLPGLDGYSIARRLREAGPPGVL
                     LVAMTGYGQDGDRQRSREAGFDVHLTKPIDPLVLDALLAKAGA"
BASE COUNT          605 a         1324 c         1350 g          550 t
ORIGIN      
        1 tcgagccaca ctgacctgtc ctcactcaca aggaggcgcg tatggaccca cgggacgatc
       61 gacggcagtt cctcaggacc gggctcgcgg cgggcacgat ggtcggtgcc gccggcctcg
      121 aaggcattct cgcggcgcgc cgcgggcccg ccttcgcgca gaccaccaag atccacctgc
      181 tgcagtgggt ggacttcatt ccggagggcg acgtcgaggt gcgacgccag gtcgccgact
      241 acacgaagca gacgaaagtc gaggtcacgt tcgagaccat caacgccaac gacctccagg
      301 cccgcatcac cgccgcgatc cagtcggggg cggggccgga cctgatcctg atgctccaca
      361 actggccgca cctctacgcc aacgccctcg tcgacgtctc ggacctgagc gactggaaga
      421 ccaaggatca gggcggctac tactcgcact gcgaggtggc gaccaaggtg ggcagccagc
      481 gcctcgcgct gccctacggc accggcggca tcctcaccgc ctaccgcaag tcgtggttcg
      541 cggaggtggg cgccctgcag ccgcccaaga cgctcgacga gtaccggaag atcgggaccg
      601 cgctcaagaa gaagggccgt ccgatcggcc agaccctcgg ccacaccttc ggggacgcgc
      661 cggcgtggac gtatccgctc ctgtggacct tcgggggcgc ggagaccgac gcgtccggcc
      721 gcaaggtggt catcaactcg aagggggcca tcgaagcggt gaagtggatg accgcgttct
      781 ggaaggacgc ctgcgacgag ggcgcgctcg cctgggacga caccaacaac aaccgggcct
      841 tccacgcggg cgagatcagc gccaccaaca acggggcctc gatctacatc ttcgccaagc
      901 gcaacccgga caagatcaag gacgagcgcg gcgagccgat gtggcgcgac atccagcact
      961 tcgcgatccc ggccgggccc aacggcacca ctccgctcta cctcgtctcg ttcgcccacg
     1021 gcctcatgaa gtactcgaag aaccagaccg cggcgcgcga gttcctcaag tggttccatt
     1081 cgaaggatca gttcggcaag tggttcgaga tcgagaacgg ctacagcgcg ggggccacca
     1141 cctactggga gacgcatccg atgtgggaca aggtcgacga ggcgctcaag gcgttccgcc
     1201 tggcccctcg cggctcgcgg atgttcggct acgcggggcc gccgagcgcg aaggcggccg
     1261 aggcctactc caagtacatc atcaccgaca tgtacgcgaa ggcggtgcag ggcatgtccg
     1321 cggaggacgc ggtgaagtgg gcggaagtcg agctgaagaa ggtctacgag gggtagtcgc
     1381 ggcgaccgcc gggcgcggac gtcccgcgcc cggcgagtcc gcgcctagcg ggccttctcg
     1441 cggtcgatca gcacccggcg caggatcttg ccggaggccg acttggggat ctggtcgatg
     1501 aactcgaggt cgcggatgcg cttgtgcggg gcgacccgct cggccaccca gcccatgatg
     1561 gcgtcggcgg gagtggcctt cgaggcgtcg tcgggcttga gcaccacgta ggccttcggc
     1621 acctcgcccg cctcctcgtc cgccttgcgc accaccgccg cgtccaggat cgccggatgc
     1681 gtgagcagca acgcctcgag ctcggcgggg gcgacctgca ggcccttgta cttgatcaac
     1741 tccttggtgc gatcgacgat gaagaagaag ccctcgtcgt ccacgtagcc gacgtcgccg
     1801 gtgtggtacc agccgtcgcg gtcgatggac tccgcggtct cgcctggacg gttgagatag
     1861 cccttcatga tctgcgggcc gcggatccac agctcgccct ccttgcccac cggtagctcc
     1921 gcgtccgagg cgccggccgc gtcgacgatc ttcacctcgg tgttcggcac gatgcagccg
     1981 gccgagccgg gcttgaacgg ggcgtcgtgg gtcgggctca ggtgggtgac cgggctcgcc
     2041 tcggtcatgc cgtagccctg gaccaccggg cagccgagcc gggcgctgag cgcgcgcgcc
     2101 atctcctcgc cgagcggggc agcccccgag aacacgaggc gcacgctcga gagatcggcc
     2161 tgggccaccg cgggatgctt caccaggccg agcacgatcg gcggcaccag gggcaggatc
     2221 gtcacccgat acttctgcac gagggcgagg aactccatca tgtcgaagcg cggcatgacc
     2281 aggatggtcc cgcccccggc gagccccagc atcatgatga ccaccatgcc gtagatgtgg
     2341 aagaacggca gcaccgccac cgtgatgtcg ctctcgtcga agcactcgaa gttcttcatg
     2401 ccctcggcct ggcagaggtt cgccaccagg ttgcgatggg tgagcatcac gcccttgggc
     2461 agcccggtgg tgccgctcga gtacggtagc gcgaccagat ccgcggccgg atcgatgggg
     2521 accgcgcgcg gcgtgtcgcc ggcccggagc aggtcggtga acgaccgcgc accctccgcg
     2581 gcgccgaaca cgaacacctc ctcgaccttg ctcttggccg ccgccgcctt cgccttctcc
     2641 aggaaggccg gcaccgtcac caggaagcgc gcgccggagt cgttgagctg ggtggccagc
     2701 tcgtcggcgg tgtagagcgg gttgacggtg gtgttgatcc cgcccgccat cgccacgccg
     2761 aggaacacca ccgcgtactc ggggaggttc gggcagtaga tggcgaagac ctgacccttg
     2821 ccgaacccgc gctgcgcgag gccggccgcg gtgcgccggg cgccctcggc gagctggcga
     2881 tagctcaggg tgcggccggt ggggccgtcg atcagggccg gcttgtcccc caggcgggcg
     2941 gcgtgacgca gcacgtagtc ggtgatcgag acgttcggga tggtgacggg agggctggtc
     3001 gagatcatgg ccaggtgagc ctagcggccc gctcggctcc ggtcaagcgc ccgctttggc
     3061 gagcagcgcg tccagcacca gcgggtcgat gggctttgtc aggtggacgt cgaagcccgc
     3121 ctcgcgcgag cgctgccggt cgccgtcctg gccgtagccg gtcatcgcca ccaggagcac
     3181 gcccggcggc ccggcctcgc gcagtcgccg ggcgatgctg tagccgtcga gcccaggcag
     3241 gccgatgtcg acgatcaccg cgtcggggct gagcgccagc gcgcgggcca gcccgtcggc
     3301 tccctcggcg gcctcgtaga cctcgtggcc tgcgtgatcg agcagatggc ggagcatctg
     3361 ccgcgcgtcg tcgttgtcct cgatgatgag cacccggcgc cggatcgcgg cgcgcggcat
     3421 ctccgggacg agcgcgctcg tgtccccggg tggccgggcc gcgggcaggc ggatggtgaa
     3481 cgcgctcccg cggccggggc ctccgctggc ggcctcgatc cggccgcggt ggagatccac
     3541 caggcgcttc accagggtga ggccgatgcc gaggccggcc tcccggcgat ggagcgtcgt
     3601 ctgcccctgc acgaagaggt cgaagatccg cggcagcagg tcggggtgga tgccggcccc
     3661 ggtgtcctcc acccgcagca ctgcctccgc gccctcgacc gtcaccgcga cggtgacccg
     3721 gccgtcggcg ggcgtgaact tgagggcgtt gccgagcagg ttgccgagga tctgctcgat
     3781 ccgggtctcg tccgcgtcga tccagaccgg cgcgtccgcg cggcatgcg
//
LOCUS       DATMCV010000088        10034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_405422, whole genome shotgun sequence.
ACCESSION   DATMCV010000088 DATMCV010000000
VERSION     DATMCV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405422"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VNU03_02175"
     CDS             complement(<1..830)
                     /locus_tag="VNU03_02175"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73282.1"
                     /translation="MATIDSRARRGLVLGGVMAAMFLAAMESTVVATAMPTVIASLGG
                     IRIYSWTFSAFLLTSTVSMPIWGRLADDLGRRPAYLGGLFLFLLGSALAGFSQTMEQL
                     IAFRAVQGLGAGSLITIGMTIVGDLYGMERRAKMQGYFSSVWGVASLVGPLVGGLLTD
                     RVSWRWVFYINLPFGLLAAAAITAGLRDEAPARPRGTFDVLGMVVFVAAISAFLVGLV
                     DAGAGETWLHSSGAALLALSGVLLVVFILVERRASEPVIPLGLFGNPIVRSAAVTGLL
                     A"
     gene            complement(830..1159)
                     /locus_tag="VNU03_02180"
     CDS             complement(830..1159)
                     /locus_tag="VNU03_02180"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HWP73283.1"
                     /translation="MTPLGKFLPIVGQPDPAQPKNVHLVGRYAIGVTWADDHGSIYPF
                     DRLRLDDQTEKTTAEGALTQEMTWPKEITKLPDALRVGWLDGHESLYPYATLRALCRC
                     AGCTGGH"
     gene            complement(1156..2064)
                     /locus_tag="VNU03_02185"
     CDS             complement(1156..2064)
                     /locus_tag="VNU03_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73284.1"
                     /translation="MASGPVDVAATLSRFHLWGEDPVNRLSDGVFRRAFKWGGRWRGY
                     ELRWSGSPDHARLTVSVPGARRAAVVDGAVAEVRHVCGLDLDLVAFRDAAAGDPVLGG
                     LATRLHGLRPTLSPSPFEMLVSSVCAQQVNLSFAFTVRARLVRRFGEALLVGGEVVYA
                     FPEPAALARAGVVELRRMQLTTRKAEYVVGLARQVVTGELDLDGLMSRSNDEVIERLT
                     AVRGFGRWSAEWFLARCLGRGDVCPAGDLAVRRAFEHFYARGRELGEPAIRRRAARWG
                     SFQNLAVHYLLAGRRLASVAAAGGGA"
     gene            complement(2196..3164)
                     /locus_tag="VNU03_02190"
     CDS             complement(2196..3164)
                     /locus_tag="VNU03_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP73285.1"
                     /translation="MSTPALRTADVSLRDALPADSDALQSLDGAAPDAGAALIQARRN
                     FFSRTAAYTRCRTFVAEQDGRIIAVNCVALIPVRVAGRPCMAGYSFNTRVASGLRSRG
                     VGTLMMRAGAEWIEQQGAPYVTGLIGVNNAASMAMVTGLGWEPVARFDYLVLDLARFA
                     PDPKVRIRKVDALGDPAHASWRFGEVFLHHFVPRYLVSDLFRPYPRGPYMGSLTAFGP
                     GGSAWLSLWDDRTRRGLDPDRIRAVKAYDVTLKGEGGFRAFSAIAATLGGMELDHLLL
                     PIPHDATARALLEPYASDIVEFNFCVKRLNGAGPLPPGPVYFDIRH"
     gene            complement(3269..5089)
                     /locus_tag="VNU03_02195"
     CDS             complement(3269..5089)
                     /locus_tag="VNU03_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWP73286.1"
                     /translation="MKISAVKKLFVGSPLATAQARHERLSRTSALAVFSSDALSSVAY
                     ATEEILLVLVLAGAAALSFSIPIGIAIALLIAVVVSSYRQTILAYPKGGGAYIVTKDN
                     LGTLPALVAAGALLIDYVLTVAVSVAAGVAAITSAFPVLYDYRILIGVLFIAGIATVN
                     LRGLRESGSLFAVPTYLFVVSFATMLAYGFVRWLFGWEGVPPPAATPAAMSQDLTIFL
                     VLRAFASGCAALTGVEAVSDGVPAFHPPEARNARIVLAWLGIILVTLFMGITFLARHY
                     QVLPRTEETVVSQLARLIFGGGLLYYEIQVVTMLILVFAANTAFADFPRLAYFLARDG
                     FIPRQFGTRGDRLVFSNGILILGGLAALLILLFGGATHALIPLYAVGVFVSFTLSQAS
                     MVRRWLSRREPGWWWRWVLNAVGAATTGLVMLVIAGTKFSHGAWMVVLLIPLLVALFM
                     MIHRHYADVARQLSLDNYGGPPSIDHSVLVLVGDLHRGVAAALRYAQTLSTSAKAVYV
                     EVDPDRTQKLEEKWGKFGLGVPLVVLTSPYRSLLTPFLDYVNHLLAVGENHVVTIVIP
                     EFVPARWWQHLLHNQTALLIKGALLFRKGVVVVDVPFHLR"
     gene            complement(5171..5371)
                     /gene="tatA"
                     /locus_tag="VNU03_02200"
     CDS             complement(5171..5371)
                     /gene="tatA"
                     /locus_tag="VNU03_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWP73287.1"
                     /translation="MFGLGAQELMLILLIALVLFGGSKIPDLGRSLGQAIREFKKGVE
                     TPDAIAEPKEGAERPKDSGPVR"
     gene            complement(5447..5737)
                     /locus_tag="VNU03_02205"
     CDS             complement(5447..5737)
                     /locus_tag="VNU03_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73288.1"
                     /translation="MLGARTLVAVLVLFHVGLLTAAAVQSADFAWVPSYFDDDDGDFL
                     PLLLSEQMSALVEPARGWMPALIVLAAVLGWRPRPRPYLVAAPARLRAPPCP"
     gene            5833..6315
                     /locus_tag="VNU03_02210"
     CDS             5833..6315
                     /locus_tag="VNU03_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HWP73289.1"
                     /translation="MNILVPDISIAEKLLRSVAVYTFLLVAFRLCGKRQLGQLSAFDL
                     VVLLIISNVVQNAVIGNDNSLGGGLLGATTILLLNLLVAYVTFRFRRADRVIEHSPTV
                     LVRHGRILRDNLRRERLGPRDLRAALRHHGVISIRDIRYAFLEEDGHVSVITRRARGG
                     "
     gene            6340..6957
                     /locus_tag="VNU03_02215"
     CDS             6340..6957
                     /locus_tag="VNU03_02215"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWP73290.1"
                     /translation="MSDLHQFVGHWGYAAIFVVVVLGNVGLPVPEETILALAGYLVWR
                     GKLRLSLVLVVGIVSAVVGDNIGYWLGRRYGQTALPRYARWVLGHPERLETMKAFVAR
                     RGPLAVFVARFVPGVRFTAGPLAGALGLRFSSFLLANVAGAVLYVPVVVGAGLAVGYG
                     FGAYVEQMRYVVGEVERTVLLLALLGILALVALRIAQAVRQHKTD"
     gene            complement(7011..7982)
                     /locus_tag="VNU03_02220"
     CDS             complement(7011..7982)
                     /locus_tag="VNU03_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007259810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HWP73291.1"
                     /translation="MESKGRTFRRLLDEEPYLFTGGIYSPLDAQIAEQAGMKSIYLSG
                     YSLAMLNGWPDMGLLTMTEVTKAASMVASAVNLPIIADADDGYGNALSTMRTVQEFVK
                     TGVAGIHLEDQRFPKRCGHIAGKTIVSREEALGKYRAAIAERDRLDRDFVIIARTDAY
                     GAVGGSMDEAIWRGRAYADAGVDLVWAELSNSEREPAIEFARAMKQSHPNLPLAFNYS
                     SSFRWHKDPNPLTFRELGELGYRFIFITLFGAHAAMYSVWNFMQELVKNEEQAQWTLE
                     RQKVGHPTESHHVMARVSHFQELERRYIPGTDERLKGSDGFGEAKSH"
     gene            complement(7997..8170)
                     /locus_tag="VNU03_02225"
     CDS             complement(7997..8170)
                     /locus_tag="VNU03_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73292.1"
                     /translation="MGSRCRCGTEIHFAFNHLGCIECGAACCPACSYQLESTTYCSSC
                     AGSLLELPPALGA"
     gene            complement(8407..9759)
                     /locus_tag="VNU03_02230"
     CDS             complement(8407..9759)
                     /locus_tag="VNU03_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP73293.1"
                     /translation="MTKVVIVGAGKGGRALLEMFSGDPTVTIVGVVDVNPWAPGLELA
                     RRLNVQVSTDLGELIADPHLDLIIDVTGNPEVQAAIQRLKPASTEVMGGASAKFMWDL
                     LAERKRTEELEDRYSLMLRELQAKSDGDFIIGQNPKMRELAEQIVRVAQTPTTVLIRG
                     ESGTGKELVARAIHRYSNLRDKPLVTVNCTALTPTLMESELFGHKRGSFTGAVADKVG
                     LFEKADGATIFLDEVGDMPPEMQGKLLRVLQAGEVKPVGDVVTRKVRVRVIAATNRDL
                     EKALATSEFREDLFYRFNAFTVVLPPLRERSEDIPVLAYHFLRKAEAKVNKKVDRFSQ
                     DALDLLRSYAWPGNLRELENIIERAVVLSSNQQVEVAHLPLYLRDASPIQVTTQEGFV
                     QAKERVVAMFEKEAVARFLSEARGNISVAAQKAGITRRNFHRLLSKYSINTKKFKVGS
                     "
     gene            complement(9776..>10034)
                     /locus_tag="VNU03_02235"
     CDS             complement(9776..>10034)
                     /locus_tag="VNU03_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HWP73294.1"
                     /translation="AVETRERKEPQLSDLRESGALEQDADVILFLYRPERYGLQSEDG
                     DRVADVVIGKQRNGPTGKVQVTFIPEYASFERLAKREAQPF"
BASE COUNT         1632 a         3482 c         3481 g         1439 t
ORIGIN      
        1 gcgagcaggc cggtgaccgc cgccgagcgc acgatcgggt tgccgaacag gcccagcggg
       61 atgaccggct ccgaggcgcg gcgctcgacc aggatgaaca ccacgagcag cacgccggac
      121 agggcgagca gtgcggcccc cgaggaatgg agccaggtct ctccggcgcc cgcgtcgacc
      181 agccccacca ggaacgccga gatcgcggcc acgaagacca ccatccccag cacgtcgaag
      241 gtcccgcgcg gccgggccgg cgcctcgtcg cgcaggcccg cggtgatcgc ggccgcggcc
      301 aggagcccga acggcaggtt gatgtagaac acccagcgcc acgacacccg atcggtcagg
      361 agcccgccca ccagcggccc gaccagcgag gccactcccc agacgctcga gaagtagcct
      421 tgcatcttcg cgcggcgctc catgccgtag aggtcgccga cgatggtcat gccgatggtg
      481 ataagggagc cggcgccgag gccctgcacc gcccggaagg cgatgagctg ctccatggtt
      541 tgcgagaacc cggccagcgc ggacccgagc agaaagagga agaggccccc cagataggcg
      601 ggccgccggc cgaggtcgtc ggcgagccgt ccccagatcg gcatcgacac cgtcgaggtg
      661 agcaggaacg cggagaaggt ccacgagtag atccggatcc cgccgaggct cgcgatcacg
      721 gtgggcatcg cggtggcgac caccgtcgat tccatcgcgg cgaggaacat ggcggccatg
      781 accccgccga gcacgagccc gcggcgggcg cgggagtcga tggtggccat cagtggccgc
      841 cggtgcagcc cgcgcagcga cacagcgctc gcagggtcgc gtacgggtag aggctctcgt
      901 ggccgtcgag ccagccgacc cggagcgcgt ccggcagctt ggtgatctcc ttgggccacg
      961 tcatctcctg cgtgagcgcg ccctcggcgg tcgtcttctc ggtctgatcg tcgaggcgca
     1021 ggcggtcgaa cgggtagatg ctgccgtgat cgtcggccca cgtcaccccg atcgcgtagc
     1081 ggccgaccag gtggacgttc ttgggctggg ccggatccgg ctgccccacg atcggcagga
     1141 acttgccgag cggcgtcatg ccccgcctcc ggccgcggcg acggacgcga gccggcggcc
     1201 ggccaggagg tagtggacgg cgaggttctg gaacgagccc caccgcgccg cgcgccggcg
     1261 aatcgccggc tcgcccagct cgcgcccgcg agcgtagaag tgctcgaagg cccggcgcac
     1321 cgcgaggtcg cccgccgggc agacgtcacc ccgcccgaga cagcgggcca ggaaccactc
     1381 cgcgctccac cgcccgaacc cgcgcaccgc cgtcaagcgc tcgatcacct cgtcgttgga
     1441 ccgcgacatc aatccgtcca ggtcgagctc acccgtcacg acttgacggg ccaagcccac
     1501 cacgtactcg gccttccgcg tcgtgagctg catgcgtcgc agctcaacga ctcccgcccg
     1561 ggccagagcc gccggctccg ggaacgcgta caccacctct ccgcccacca gtaatgcctc
     1621 accgaagcgc cggaccagcc gcgccctcac cgtgaacgcg aagctcaggt tgacctgctg
     1681 cgcgcacacc gagctcacca gcatctcgaa cgggctgggc gacagcgtcg gcctgagccc
     1741 gtggagccgg gtggccaggc cgcccagcac cgggtccccc gccgccgcgt cacggaacgc
     1801 gaccagatcc aggtccagtc cacacacgtg ccgcacttcc gcgaccgcgc cgtcgacgac
     1861 cgcggcgcga cgggccccgg gaacggagac ggtcagccgc gcgtgatcgg ggcttccgct
     1921 ccagcgcagc tcgtagccgc gccagcgacc gccccacttg aacgcacggc ggaacacgcc
     1981 atcggagagc cggttgaccg gatcttcgcc ccagaggtga aaccgcgaca gggtcgcggc
     2041 cacatccacc gggcccgagg ccacgcccga ggccgcgcca gagactacga acgtcgcgtc
     2101 catagacaac gcggccaagt gtagcagagg cgtcccggga gcggggcacg caacggaccg
     2161 cgcgcgggag ctggcccgcc ccgcggccgc gccgctcagt ggcggatgtc gaagtagacg
     2221 ggtccggggg ggagcgggcc ggcgccgttg aggcgcttga cgcagaagtt gaactcgacg
     2281 atgtcgctgg cgtacggctc gagcagcgcc cgggcggtcg cgtcgtgcgg gatcgggagc
     2341 aacagatggt cgagctccat cccgcccagg gtggccgcga tggccgagaa cgcgcggaag
     2401 ccaccctcgc ccttcaaggt gacgtcatag gccttcaccg cccggatccg gtccgggtcg
     2461 aggccgcgcc gggtccggtc gtcccacagg gacaaccagg cgctgccccc cggcccgaag
     2521 gcggtgaggc tccccatgta cggtcccctg gggtatggcc ggaagaggtc ggagacgagg
     2581 tatcgcggga cgaagtggtg aaggaacacc tccccgaagc gccagctggc gtgggcgggg
     2641 tcgccgaggg cgtcgacctt ccggattctc actttcggat cgggggcgaa gcgggccagg
     2701 tcgagcacca ggtagtcgaa gcgcgcgacc ggctcccagc ccagcccagt caccatcgcc
     2761 atcgaggccg cgttgttgac cccgatcagc ccggtgacgt agggggctcc ctgctgctcg
     2821 atccactcgg cccccgccct catcatcagg gtgcccacgc cgcgagagcg caagccggag
     2881 gcgacccggg tgttgaagct gtagccggcc atgcacggtc gtccggccac ccgcaccggg
     2941 atgagcgcca cgcagttgac cgcgatgatc cgcccgtcct gctcggccac gaaggtccgg
     3001 cagcgcgtgt aggcagcggt gcgggagaag aagttccggc gggcctggat cagggctgcg
     3061 ccggcatcgg gcgccgcgcc gtccagtgac tgcagggcat cactgtcggc cggaagggcg
     3121 tctcggagcg aaacatccgc ggtccggagg gccggagtac tcatgagctt cgcccaacaa
     3181 tactcgcctt ttggccgtcc gcaacttttg catgtgaagg gtcctgctat ggtgccgctt
     3241 gcctaccgcc cgctgcctac cgcccgccct accggaggtg gaacgggacg tcgacgacga
     3301 cgacgccttt ccggaagagc agcgctccct tgatgaggag ggcggtctgg ttatggagca
     3361 ggtgctgcca ccagcgcgcg gggacgaact cggggatgac gatggtgacc acgtggttct
     3421 cgcccaccgc caggagatgg ttgacgtagt cgaggaaggg cgtgagcagc gaccggtagg
     3481 gcgaggtcag caccaccagc gggaccccca gcccgaactt gccccacttc tcctcgagct
     3541 tctgggtccg gtcggggtcg acctcgacgt acaccgcctt cgcggacgtg gagagggtct
     3601 gcgcgtagcg cagggccgcc gccacgcccc ggtggaggtc gcccaccagg accagcaccg
     3661 agtgatcgat cgacggcggg cccccgtagt tgtcgaggga gagctgccgg gccacgtcgg
     3721 cgtagtggcg gtggatcatc atgaacagcg ccaccagcag cgggatgagc agcaccacca
     3781 tccacgcgcc gtggctgaac ttggtgcccg cgatcacgag catcacgagc ccggtggtgg
     3841 cggctccgac cgcgttgagc acccagcgcc accaccagcc cggctcgcgc cgcgagagcc
     3901 accgccgcac catgctggcc tgcgagaggg tgaaggagac gaagacgccg accgcgtaga
     3961 gcgggatgag ggcgtgggtg gcgccgccga agaggaggat caggagcgcg gccaggccgc
     4021 ccaggatcag gatcccgttc gagaacacga gccggtcccc gcgggtgccg aactggcgcg
     4081 ggatgaaccc gtcgcgggcg aggaagtacg cgaggcgggg aaagtcggcg aacgcggtgt
     4141 tggccgcgaa caccaggatc agcatggtca cgacctggat ctcgtagtag aggaggccgc
     4201 ctccgaatat caggcgcgcg agctgcgaga cgacggtctc ctccgtgcgg ggcagcacct
     4261 ggtagtgccg ggccaggaag gtgatgccca tgaacagcgt gaccaggatg atgccgagcc
     4321 aggcgagcac gatgcgcgcg ttgcgcgctt ccggcggatg gaacgcgggc accccgtcgg
     4381 acaccgcctc cacgccggtc agcgccgcgc agccggacgc gaaggcgcgg agcaccagga
     4441 agatcgtgag gtcctggctc atcgcggcgg gggtcgcggc gggcggggga acgccctccc
     4501 atccgaacaa ccagcgcacg aagccgtagg cgagcatggt cgcgaagctc accacgaaca
     4561 gatacgtcgg caccgcgaag aggctgcccg actcgcgcag gccgcgcagg ttcaccgtcg
     4621 cgatccccgc gatgaagagc accccgatga ggatgcggta gtcgtagagg acggggaacg
     4681 cggaggtgat cgcggcgact ccggccgcga cgctcaccgc caccgtgagc acgtagtcga
     4741 tgagcagggc ccccgcggct accagcgcgg gcagcgtgcc caggttgtcc ttggtgacga
     4801 tgtaggcgcc accccccttc gggtaggcga ggatcgtctg gcggtacgag ctcaccacca
     4861 ccgcgatgag gagggcgatc gcgatcccga tcgggatcga gaagctgagc gcggcggctc
     4921 cggcgagaac cagcaccaac aaaatttcct cggtggcgta tgccaccgag gacaacgcgt
     4981 cggaggagaa gaccgcgagg gcggacgtcc gggacagccg ctcgtgccgg gcctgggccg
     5041 tggccagcgg cgacccgacg aacagcttct tgaccgcgct gatcttcacc cgggcagcct
     5101 ctggatcatc aacgctagag gcggcggcgc ccgggtgtca agcattcgcg cggccccgag
     5161 gtggcgcgcg ctaccggacg gggccggaat ccttcgggcg ctccgcgccc tccttcggct
     5221 cggcgatcgc gtccggcgtc tccacgccct tcttgaactc gcggatcgcc tgccccagcg
     5281 accgacccag gtcgggaatc ttcgatccac cgaacagcac cagcgcgatg agcaggatca
     5341 gcatcagctc ctgagcaccc agtccgaaca tgtgcatcct cctcgaatcg agagtccacc
     5401 accgtgcgac cggcggtccg gccgcgtgca ctgcgtgggg aggaggctat ggacagggcg
     5461 gagcccgcaa acgggcgggc gcggcgacga ggtaggggcg cggccgcggg cgccacccga
     5521 ggaccgccgc gagcacgatc agcgcgggca tccacccgcg ggcgggctcg accagcgccg
     5581 acatctgctc ggagagcagg agcggcagga agtcgccgtc gtcgtcgtcg aagtagctcg
     5641 ggacccacgc gaagtcggcc gactggaccg cggcggccgt gagcagcccc acgtggaaca
     5701 gcaccagcac cgccaccagg gtgcgcgctc cgagcatggc gccgagccta cggccgcggg
     5761 gtccgcgtgt caaattcggc gtcgaaccgt cggggccggt gcgatgctgt gggcaaggag
     5821 ccggaggcgg acatgaacat cctggtaccc gacatcagca tcgcggaaaa gctgctccgc
     5881 tcggtggccg tgtacacgtt cctcctcgtc gcctttcgcc tgtgcggcaa gcggcagctc
     5941 ggccagctga gcgccttcga cctcgtggtg ctcctcatca tcagcaacgt ggtgcagaac
     6001 gcggtgatcg gcaacgacaa ctccctcggg ggcgggctcc tcggcgccac caccatcctc
     6061 ctcctcaacc tgctggtcgc gtacgtgacc tttcgcttcc ggcgggccga ccgcgtgatc
     6121 gagcactccc cgaccgtgct cgtccggcac ggccgcatcc tgcgcgacaa cctgcggcgc
     6181 gagcgcctgg gccctcggga tctccgcgcc gccctgcggc atcacggggt gatctcgatc
     6241 cgggacatcc gctatgcctt cctcgaggag gacgggcacg tgagcgtcat caccaggcgt
     6301 gcgcgcggcg gctgagtccg ccgcatccat aaccgcttcg tgagcgatct ccaccagttc
     6361 gtcgggcact ggggctatgc cgccatcttc gtggtcgtcg tgctcggcaa cgtcggcctt
     6421 ccggtgcccg aggagaccat cctggccctc gccggctacc tggtctggcg gggcaagctg
     6481 cgcctctccc tggtgctggt ggtcggcatc gtgagcgcgg tggtcggcga caacatcggc
     6541 tactggctcg gccggcggta cggccagacc gcgctgcccc gctacgcccg ctgggtgctc
     6601 ggccatcccg agcggctcga gaccatgaag gccttcgtgg cccgccgggg ccccctggcc
     6661 gtgttcgtgg cccgcttcgt cccgggggtg cgcttcaccg cggggcccct cgcgggggcc
     6721 ctgggcctgc ggttctcctc gttcctgctc gcgaacgtgg cgggggcggt gctctacgtg
     6781 ccggtggtcg tgggcgcggg gctggcggtg ggctacgggt tcggcgccta cgtggagcag
     6841 atgcgctacg tggtcggcga ggtggagcgc accgtcctgc tcctggcact gctgggcatc
     6901 ctggccctgg tcgcgctccg gatcgcgcag gccgtccgcc agcacaagac ggactgaggc
     6961 cgcggcgacc ggacccggag agcgcccggt cgccgcggag accggctcga tcagtggctc
     7021 ttcgcttccc cgaagccgtc ggagcccttg aggcgctcgt cggtgcccgg gatgtaccgg
     7081 cgctccagct cctggaagtg cgacacccgg gccatcacgt gatggctctc ggtgggatgg
     7141 cccaccttct gccgctcgag ggtccactgg gcctgctcct cgttcttcac cagctcctgc
     7201 atgaagttcc acaccgagta catcgcggcg tgggcgccga agagggtgat gaagatgaag
     7261 cggtagccca gctcgccgag ctcgcggaac gtgagggggt tgggatcctt gtgccagcgg
     7321 aaggacgacg agtagttgaa ggccagcggc aggttcgggt gcgactgctt catcgcacgc
     7381 gcgaactcga tggccggctc gcgctccgag ttcgacagct cggcccacac caggtcgacc
     7441 cccgcgtcgg cgtaggcgcg accccgccag atcgcctcgt ccatgctccc ccccaccgcc
     7501 ccgtacgcgt cggtgcgggc gatgatcacg aagtcgcggt cgagccggtc acgctccgcg
     7561 atcgcggccc ggtacttgcc gagggcctcc tcgcgcgaca cgatggtctt gcccgcgatg
     7621 tggccgcagc gcttcgggaa gcgctggtcc tccaggtgga tgcccgcgac gccggtcttg
     7681 acgaactcct gcaccgtccg catggtcgac agcgcattgc cgtagccgtc gtccgcgtcg
     7741 gcgatgatcg gcaggttcac cgcgctcgcc accatcgagg cggccttggt cacctcggtc
     7801 atggtgagaa ggcccatgtc gggccagccg ttgagcatgg ccagcgagta gccgctcagg
     7861 tagatcgact tcatccccgc ctgctccgcg atctgcgcat cgagcggcga gtagataccg
     7921 ccggtgaaga gatagggctc ctcgtcgagg agccgccgga acgttctgcc cttggattcc
     7981 atgaatgagc ctccttctag gcgccgagcg cgggcggcag ctccagcagg ctaccggcgc
     8041 agctcgagca gtaagtcgtg gactcgagct ggtaggagca agccgggcag caggccgccc
     8101 cacactcgat acagccgagg tgattgaagg cgaagtggat ctcggtcccg cagcggcaac
     8161 ggctccccat ggagctcctc cttgagccag agatatctcg aggagagaca agaaccgttc
     8221 caccgcgctg cgacccagaa gccgttgttt cgacaggcta tgtgcagccg attgccgtcc
     8281 gagatgtgac agccgtgtct cactcagagg cggggtaagg ttacgtacag aggccgaacc
     8341 agcggataaa tatgagctat tgcagcatgg ctcgcatggg tcgtatatga cccatatgcg
     8401 agtcgactac gaccctacct tgaatttctt cgtgtttatc gagtacttgg acaggagccg
     8461 gtggaagttt ctccgggtaa tgccggcctt ctgagcggcc accgagatgt tcccgcgcgc
     8521 ctcggacagg aaacgggcca cggcctcctt ctcgaacatc gcgaccaccc gctccttggc
     8581 ctgcacaaac ccctcctggg tcgtgacctg gatggggctc gcgtcccgca gatagagcgg
     8641 caggtgggcg acctccacct gctggttcga ggacagcacc accgcccgct cgatgatgtt
     8701 ctcgagctcc cgcaggttgc cgggccaggc gtagctccgc aggaggtcca gggcatcctg
     8761 cgagaagcgg tcgaccttct tgttcacctt cgcctcggcc ttgcggagga agtgataggc
     8821 caggaccggg atgtcctcgc tccgctcccg cagcggcggc aggaccaccg tgaaggcgtt
     8881 gaagcggtag aagaggtcct cgcggaactc ggaggtggcg agcgccttct cgaggtcgcg
     8941 gttggtggcc gcgatgaccc gcacccgcac cttgcgggtc accacgtcac cgaccggctt
     9001 cacctccccg gcctgcagga cccgaagcag cttcccctgc atctcggggg gcatgtcgcc
     9061 gacctcgtcc aggaagatgg tcgccccgtc cgccttctcg aacaggccga ccttgtccgc
     9121 caccgccccg gtgaacgacc cccgcttgtg cccgaagagc tcggattcca tgagggtcgg
     9181 ggtcagcgcg gtgcagttga cggtgaccag tggcttgtcg cgcaggttcg agtaccgatg
     9241 gatggcgcgc gccaccagct ccttgccggt accggattct ccgcggatga gcaccgtggt
     9301 cggagtctgg gccacccgga cgatctgctc ggcgagctcc cgcatcttcg ggttctgccc
     9361 gatgatgaag tcgccgtccg acttggcctg cagctcccgg agcatcaggg agtagcggtc
     9421 ctccagctcc tcggtccgct tgcgctcggc cagcaggtcc cacatgaact tggcgctcgc
     9481 cccgcccatg acctcggtgc tcgccggctt caaccgctgg atggcggcct gcacctccgg
     9541 attgccggtc acgtcgatga tcaggtcgag gtggggatcc gcgatgagct cacccagatc
     9601 ggtgctcacc tggacgttga ggcggcgggc cagctcgagg cccggcgccc acgggttcac
     9661 gtcgacgacg cccacgatgg tcacggtggg atcgccgctg aacatctcga gcaaggcgcg
     9721 gccgcccttg cccgccccga cgatgacgac cttggtcacc gtcccggagg cgcggctaga
     9781 agggctgcgc ctcgcgcttg gcgaggcgct cgaaggaggc gtactcgggg atgaaggtca
     9841 cctgcacctt gccggtgggg ccgttgcgct gcttgccgat gaccacgtcc gcgacccggt
     9901 cgccgtcctc cgactgcagg ccgtagcgct ccgggcgata gaggaagagg atgacgtcgg
     9961 cgtcctgctc gagcgcaccc gactcccgca ggtccgagag ctggggctcc ttgcgctcgc
    10021 gggtctccac cgcg
//
LOCUS       DATMCV010000089         2392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_604381, whole genome shotgun sequence.
ACCESSION   DATMCV010000089 DATMCV010000000
VERSION     DATMCV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604381"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..1011)
                     /locus_tag="VNU03_02240"
     CDS             complement(358..1011)
                     /locus_tag="VNU03_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWP73295.1"
                     /translation="MSSAAIFDMDGVLIDSGAHHRHAWRMLLDELGAEPADPEHWRLT
                     IGRPSEEAIPLLLGRRVSGAEARRLARRKRDFYQARAQTGLDPVAGVREFLEALARDD
                     VPRAVGTSASRWDAERLLDDLGLLRFFDVMVTADDVMLGKPDPEVWTQAARRLRVAVQ
                     RCVVFEDALVGVQAARSAGMRVIGVTTAHTDAELLAAGAERTIPDFQGLEWSQLAHR"
     gene            complement(1150..1779)
                     /locus_tag="VNU03_02245"
     CDS             complement(1150..1779)
                     /locus_tag="VNU03_02245"
                     /inference="COORDINATES: protein motif:HMM:NF018084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1054 family protein"
                     /protein_id="HWP73296.1"
                     /translation="MSFTGFTPSDFKVFEIPGFKPRMDAIKTRIRPKLEAVGRELLPD
                     VARVGGAEAFAHVARHARRTVNPPDDTWVAFALDQRGYKKHCHFKIAVSRGGVRFLFE
                     AGPEHAQKKRWVAAWKRHTRQLVPVLRRAKDLGWFKDEHDEAPAAVLTDLPADAVALL
                     GDELLRTRDGQFVLGRRVPAEQAVKWKPREYARAARETFHLLAPLYRLR"
     gene            complement(1796..>2392)
                     /locus_tag="VNU03_02250"
     CDS             complement(1796..>2392)
                     /locus_tag="VNU03_02250"
                     /inference="COORDINATES: protein motif:HMM:NF018829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HWP73297.1"
                     /translation="PIVLADMADNTGGGAAGDGTEILRELLRVGARDATVACLWDAAA
                     VQVCAKAGAGARVTLEIGGKVDDRHGAPLRVTGTVRTLSDGRFVHRGPMMTGLPGRLG
                     ATAVLDIDDVKVILITYRWQTLDPEMIRFVGIDPLSEKILVVKSTIHYRAAFEPIAKE
                     IIEVDAPGLSSSNLARFDFRRIRRPIFPLDPDLVYEAE"
BASE COUNT          341 a          865 c          868 g          318 t
ORIGIN      
        1 ccgccaccgc ggcctccgcg aagtcgaacg cggtgacccg atagccccgc cgggccagga
       61 gccgcgcatc gtgcccgcgc ccagcgcccg ggacggccac ccgcggacca ccgagcgcgg
      121 tacccgagac ggttgtccgc ggcagggggg aaacggttgt ccgcagctgg gggagtgcgg
      181 gggccatttc tgggcccccg cagatgaagg gccttgaaga actagacggt tgtccgccga
      241 acgcggcctc gaactggcca cccccatcga gccagtcgac cagcgccggc gcggcctcgc
      301 ccaggtccca gccgtcctgg ccgaccgcgt agagaccgtc ccagaacgcg gcgtggctta
      361 ccggtgagcg agctggctcc actcgagccc ctggaaatcg ggaatggtgc gctccgcgcc
      421 cgcggcgagg agctcggcgt cggtgtgcgc ggtggtgact ccgatcacgc gcatgcccgc
      481 cgagcgcgca gcctgcacgc cgacgagcgc gtcctcgaac accacgcagc gctgcaccgc
      541 cacccgcagg cggcgcgcgg cctgcgtcca cacctcgggg tcgggcttgc ccagcatcac
      601 gtcgtcggcg gtgaccatca cgtcgaagaa gcgcaggagc ccgagatcgt cgaggagccg
      661 ctcggcgtcc cagcgcgagg cggaggtgcc gaccgcgcgc ggcacgtcgt cccgcgcgag
      721 ggcctcgagg aactcgcgca cgcccgccac cggatcgagc ccggtctgcg cgcgcgcctg
      781 gtagaagtcg cgcttgcggc gggccagccg ccgggcctcc gccccgctca cccggcgccc
      841 gagcaggagc gggatcgcct cctcgctggg ccggccgatg gtgagacgcc agtgctcggg
      901 atcggcgggc tcggcgccca gctcgtccag cagcatccgc cacgcgtgcc gatgatgcgc
      961 tccactgtcg atcagcaccc cgtccatatc gaagatcgcc gcgctactca ccgcgaacca
     1021 ttgtgcgccg acggggggag tgcgggggcc attcctgggc cccacgggtt gtgcgccgac
     1081 ggggggagtg cgggggccat tcctgggccc ccgcagctga agagcttgaa gaagtaagcg
     1141 gtcgtccgct caccgcaacc ggtacagcgg cgccagcagg tggaacgtct cgcgcgcggc
     1201 gcgcgcgtac tcgcgcggct tccacttcac cgcctgctcg gcggggacgc ggcggcccag
     1261 cacgaactgg ccgtcgcggg tgcgcagcag ctcgtcgccg agcagcgcca ccgcgtccgc
     1321 gggcaggtcg gtgaggacgg cggccggggc ctcgtcgtgc tcgtccttga accagccgag
     1381 atccttggcc cggcgcagca ccggcacgag ctggcgagtg tggcgcttcc acgccgcgac
     1441 ccagcgcttc ttctgggcgt gctcggggcc ggcctcgaag aggaagcgga ccccgccgcg
     1501 ggagaccgcg atcttgaagt ggcagtgctt cttgtagccc cgctggtcga gggcgaaggc
     1561 cacccaggtg tcgtcgggcg ggttcacggt gcgccgcgcg tgccgggcca cgtgggcgaa
     1621 ggcctcggcc ccgcccacgc gggccacgtc ggggaggagc tcgcggccga ccgcctcgag
     1681 cttggggcgg atgcgggtct tgatcgcgtc catgcggggc ttgaaccccg ggatctcgaa
     1741 taccttgaag tcggacgggg tgaacccggt gaaggacatg ctgggccctc tccttctact
     1801 ccgcctcgta caccaggtcg gggtcgagcg ggaagatggg gcgccggatg cgccggaagt
     1861 cgaagcgcgc caggttggac gacgagaggc ccggggcgtc gacctcgatg atctccttgg
     1921 cgatgggctc gaaggccgcg cgatagtgaa tggtcgactt cacgaccagg atcttctccg
     1981 agagggggtc gatgcccacg aaccggatca tctccggatc gagggtttgc cagcggtagg
     2041 tgatcagtat caccttcacg tcgtcgatgt ccagcaccgc ggtggcgccg agacggccgg
     2101 gcagtcccgt catcatcggc ccacggtgca cgaagcgccc gtcggagagc gtgcgcaccg
     2161 tgccggtcac gcgcagcggc gcgccgtggc gatcgtccac cttcccgccg atctcgaggg
     2221 tgacgcgcgc gcccgcgccc gcctttgcgc acacctgcac cgccgccgcg tcccacagac
     2281 aggccaccgt cgcgtcgcgc gcgcccaccc ggagcagctc gcgcaggatc tcggtgccgt
     2341 cgcccgccgc gccgccgccg gtgttgtcgg ccatgtcggc gagcacgatg gg
//
LOCUS       DATMCV010000090         2063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_738006, whole genome shotgun sequence.
ACCESSION   DATMCV010000090 DATMCV010000000
VERSION     DATMCV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738006"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VNU03_02255"
     CDS             complement(<1..856)
                     /locus_tag="VNU03_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HWP73298.1"
                     /translation="MKLPAEGKTRADVMALLERSMAGDLAWHEGRTFSLVYGAGEEHL
                     RLLREAYALFMATNGLGAGRIFKSLSSLEADLVGMSAELLGGAGCPGNVTSGGSESII
                     LGIRAARERARAERPRVTRPELVLPVSAHPAFDKAAEIFGIRNVRTPITKDYVADVQA
                     MADAVTDDTIGLVASAPNYPFGTIDPVPAVAEIARRHALHLHVDACVGGFALPFLRKL
                     GQPIPPFDFAVPEVSTMSADLHKYGFAARGTSLILYRDQALQSRVAFQLDDWAGGPYR
                     TPTLAGSRP"
     gene            complement(853..1650)
                     /locus_tag="VNU03_02260"
     CDS             complement(853..1650)
                     /locus_tag="VNU03_02260"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73299.1"
                     /translation="MARRLLIAYGVLGFAFLYLPIAFLVLFSFNDNIIPSLPFRGFTL
                     QWYRELGFDFVLHDALYNSLVIASITTVTSTVIGTLAAFPLVRSTFALKRAAQAMMIV
                     PMVIPHFLMGVALLLFFAFVHLPLSRLTVVLGHVVFTVPFAILIVSTRLYGFDRSLER
                     AAGDLGATPARVFWHVTLPLILPGVMGAALFVFTLSMDEFLITFFVSGQKGTLTMYIW
                     SLLKDGIAPRVNALASVLLAASFALLLVGGWLLDRGAARHAAQGRTP"
     gene            complement(1660..>2063)
                     /locus_tag="VNU03_02265"
     CDS             complement(1660..>2063)
                     /locus_tag="VNU03_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP73300.1"
                     /translation="SPFSVVLVMTYFSLPFAVVALYSSLEKMDWALLDAAGDLGARPL
                     RAFWHVTLPQTVPGVVSAAALTFVPLVGMFFVPLLVGGSTSVMIAPLIANQMQAFQLG
                     LGAAMSFIVALIVLAALALPWRYLDLERLER"
BASE COUNT          385 a          663 c          771 g          244 t
ORIGIN      
        1 ccgggcgcga gccggccagg gtgggcgtgc gatagggccc gccggcccag tcgtcgagct
       61 ggaacgccac ccggctctgg agcgcctggt cccggtacag gatcagcgag gtgccgcgcg
      121 ccgcgaagcc gtacttgtgc aggtccgccg acatcgtcga cacctcgggg accgcgaagt
      181 cgaacggcgg gatgggctgg cccagcttcc ggagaaacgg cagggcgaag ccgcccacgc
      241 acgcgtcgac gtggagatgg agcgcgtgcc ggcgggcgat ctcggcgacg gcgggcaccg
      301 gatcgatcgt gccgaacggg tagttgggcg ccgaggccac gaggccgatg gtgtcgtcgg
      361 tcaccgcgtc ggccatcgcc tgcacgtcgg cgacgtagtc cttggtgatg ggggtccgga
      421 cgttccggat gccgaagatc tccgccgcct tgtcgaaggc gggatgcgca ctcaccggca
      481 gcaccagctc cgggcgggtg acccgcggcc gctcggcgcg cgcccgctca cgggcggcgc
      541 ggatgcccag gatgatggac tccgatccgc cggaggtgac gttgccggga cagccggcgc
      601 cgccgagcag ctcggcgctc atgcccacca ggtcggcttc cagcgacgag aggctcttga
      661 agatgcgccc cgccccgagc ccgttggtgg ccatgaagag cgcgtacgcc tcgcgcagga
      721 gccgcaggtg ctcctcgccc gcgccgtaga ccaggctgaa cgtccgaccc tcgtgccagg
      781 cgagatcgcc ggccatggac cgctcgagca atgccatcac gtccgcacgc gtctttcctt
      841 cggcaggcag cttcacggtg tcctcccctg ggccgcgtgt cgagcggccc cacggtcgag
      901 cagccacccg cccaccagca ggagggcgaa ggaggcggcc aggagcacgc tggcgagggc
      961 attcacccgg ggggcgatgc cgtccttcag caggctccag atgtacatgg tgagcgttcc
     1021 cttctggccg gagacgaaga aggtgatgag gaactcgtcc atcgagagcg tgaacacgaa
     1081 gagcgcggcg cccatcaccc cgggcaggat cagcggcagg gtcacgtgcc agaacacccg
     1141 ggccggcgtg gcaccgaggt cgcccgcggc ccgctccagc gagcggtcga agccgtacag
     1201 gcgcgtcgac acgatgagga tcgcgaacgg gacggtgaac acgacgtgac ccagcacgac
     1261 ggtgagccgg gacagcggca ggtgcacgaa ggcaaagaag agcagcaggg ccacgcccat
     1321 caggaagtgc gggatcacca tcggcacgat catcatggcc tgggccgctc gcttgagcgc
     1381 gaaggtggag cgcaccaggg gaaaggcggc cagcgtcccg atcacggtcg acgtcacggt
     1441 ggtgatggag gcgatgacga ggctgttgta gagcgcgtcg tgcagcacga agtcgaagcc
     1501 gagctcccgg taccactgca gggtgaaccc ccggaagggc agcgacggga tgatgttgtc
     1561 gttgaacgag aacaggacga ggaaggcgat gggcaggtag aggaacgcga agcccagcac
     1621 gccgtatgcg atgagaagcc gcctcgccat ggtcgtccgc tagcgctcca ggcgctccag
     1681 atcgaggtag cgccacggca gggccagcgc ggccaggacg atcagggcga cgatgaagga
     1741 catggcggcg cccaggccga gctggaaggc ctgcatctgg ttggcgatga ggggggcaat
     1801 catgaccgac gtggacccgc cgacgagaag cggcacgaag aacatcccga ccaggggcac
     1861 gaaggtgagc gcggcggccg agaccacgcc gggaaccgtc tggggcaggg tgacgtgcca
     1921 gaaggcgcgg agcggccgcg cccccaggtc ccccgccgcg tcgagcagcg cccagtccat
     1981 cttctcgagc gacgagtaga gggcgaccac cgcgaagggc agggagaagt aggtcatgac
     2041 gagcacgacc gagaacgggc tgt
//
LOCUS       DATMCV010000091         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_5407, whole genome shotgun sequence.
ACCESSION   DATMCV010000091 DATMCV010000000
VERSION     DATMCV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5407"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VNU03_02270"
     CDS             <1..295
                     /locus_tag="VNU03_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate dehydrogenase domain-containing
                     protein"
                     /protein_id="HWP73301.1"
                     /translation="LVFEGPATEAVRGFPANVNVVAALSLAGIGPERTRIKIYAVPGQ
                     ARNQHRITVEGEFGTLRIEVENVPSENPRTGRLSYLSAIAMLRELGAAVQVGN"
     gene            314..739
                     /gene="mscL"
                     /locus_tag="VNU03_02275"
     CDS             314..739
                     /gene="mscL"
                     /locus_tag="VNU03_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWP73302.1"
                     /translation="MLKEFKEFAIKGNMVDMAVGIIIGAAFGTVVTSLVNDLLMPVIS
                     GLIGTPDFSNLFAILRNPTGAALTSVKAARDAGAVVLAFGLFLNAIIAFLAVAVALFF
                     LVKVVNRMRRQEEAAPAAPPAPSEEVKLLAEIRDLLRRR"
     gene            736..1032
                     /locus_tag="VNU03_02280"
     CDS             736..1032
                     /locus_tag="VNU03_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73303.1"
                     /translation="MIRPSRRARTAALLLAAAAGAGCEYFRDSPSLELANRRWRECTS
                     GLRDVQLQRVDVDGRIRFTYVAINERDAALACLAAAARSERALPEPVASAIAGK"
     gene            complement(1086..2024)
                     /gene="trxB"
                     /locus_tag="VNU03_02285"
     CDS             complement(1086..2024)
                     /gene="trxB"
                     /locus_tag="VNU03_02285"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWP73304.1"
                     /translation="MATTRKVVIIGSGPSGYTAAIYAARANLSPVMFTGIQPGGQLML
                     TTLVENYPGFVEGIDGPPLMETFQKQAERFGTEMIADDVTEVDFRKRPFTVTGGDVTI
                     EAHTVILATGASAKLLGLPNESKLMGRGVSTCATCDGFFFKDQNIMVVGGGDSALEEA
                     LYLSRLGRKVDLVHRREALRGSKIMQERAFKNPKITFVWNTVVEDVLDPSAGKVTTVK
                     LRNIKTGVASESQVDGLFVAIGHQPNTALVKGQVDLHPNEYIKVLPGATQTSVAGVFA
                     AGDVADFTYRQAVTAAGTGCMAALEAERYLEAQGLE"
BASE COUNT          294 a          703 c          708 g          320 t
ORIGIN      
        1 gctcgtcttc gagggccccg cgaccgaggc ggtccgcggc ttcccggcca acgtcaacgt
       61 ggtggccgcg ctctctctgg ccggcatcgg ccccgagcgc acccggatca agatctacgc
      121 ggtgcccggt caggctcgga accagcaccg gatcacggtg gagggcgaat tcggaaccct
      181 ccgcatcgag gtggagaacg tgccctcgga gaacccacgg accggccggc tctcctacct
      241 atccgcgatc gcgatgctgc gcgagctggg cgccgcggtg caggtgggta actgagccca
      301 aggagggacg cgcatgctca aggagttcaa ggaattcgcg atcaagggca acatggtgga
      361 catggcggtc ggcatcatca tcggcgccgc cttcggcacg gtggtcacct cgctggtcaa
      421 cgatctcctc atgcccgtca tctcggggct catcggcacg ccggacttca gtaacctgtt
      481 cgcgatcctg cgcaacccga ccggcgccgc cctcacgtcg gtcaaggccg cgcgcgacgc
      541 gggcgcggtg gtgctcgcgt tcgggctctt cctgaacgcg atcatcgcgt tcctcgcggt
      601 cgcggtggcc ctcttcttcc tcgtgaaggt ggtcaaccgg atgcggcgac aggaagaggc
      661 cgcgcccgcc gccccgcccg cgccctccga agaggtcaag ctgctggccg agatccgcga
      721 cctgctgcgt cggcggtgat acggccctcg cggcgcgccc ggaccgccgc cctcctgctg
      781 gcggcggcgg ccggggcggg ctgcgagtac ttccgagaca gcccttcgct ggagctggcc
      841 aaccggcgct ggcgggagtg cacgtcgggg ctccgcgacg tccagctcca gcgggtggac
      901 gtggacggcc ggatccgctt cacctacgtg gcgatcaacg agcgcgacgc ggccctcgcg
      961 tgcctggccg cggccgcgcg atccgagcgc gcgctgccgg agccggtcgc gagcgctatc
     1021 gcgggaaagt agcaggggaa agtagcgacc gcgggcccga tcgggcccgc cgcgcccctc
     1081 ccttcttact cgagcccctg cgcctccagg tagcgctcgg cctcgagcgc cgccatgcag
     1141 ccggtgcccg cggcggtcac cgcctgccga tacgtgaagt cggccacgtc gccggccgcg
     1201 aacacgccgg ccaccgaggt ctgggtggcc ccggggagca ccttgatgta ctcgttgggg
     1261 tgcaggtcga cctggccctt gacgagggcg gtgttgggct ggtgcccgat ggccacgaag
     1321 agcccgtcca cctgcgactc ggaggcgacg ccggtcttga tgttccggag cttgaccgtc
     1381 gtcaccttgc ccgcggacgg atccaggacg tcctccacca ccgtgttcca gacgaacgtg
     1441 atcttcgggt tcttgaacgc ccgctcctgc atgatcttgg acccgcgcag cgcctcgcgg
     1501 cgatgcacga gatcgacctt gcggccgagc cgcgagaggt agagggcctc ctcgagcgcc
     1561 gagtccccgc ccccgaccac catgatgttc tgatccttga agaagaaccc gtcgcaggtg
     1621 gcgcaggtcg agaccccgcg acccatgagc ttggactcgt tggggaggcc caggagcttg
     1681 gcggacgcgc cggtggcgag gatgacggtg tgcgcctcga tggtgacgtc gcccccggtg
     1741 accgtgaagg gccgcttgcg gaagtcgacc tcggtcacgt cgtcggcgat catctcggtg
     1801 ccgaagcgct cggcctgctt ctggaacgtc tccatcagcg gaggtccgtc gatgccctcg
     1861 acgaagcccg gatagttctc gaccagggtg gtcagcatga gctggccgcc cggctggatg
     1921 ccggtgaaca tgaccgggga caggttggcg cgcgccgcgt agatggccgc ggtgtagccg
     1981 gacggtcccg atccgatgat gacgaccttg cgtgtcgtgg ccacg
//
LOCUS       DATMCV010000092         2082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_72045, whole genome shotgun sequence.
ACCESSION   DATMCV010000092 DATMCV010000000
VERSION     DATMCV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72045"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VNU03_02290"
     CDS             complement(<1..593)
                     /locus_tag="VNU03_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73305.1"
                     /translation="MRVRHLLVLLAVLAYPWVATPFFTFQIGAQALALGLIALSLTFL
                     AGYGGMVSLAQMTVAGIAGYMYAIFATSGTDISLRWPWGLAVVLALVIATICATLIGW
                     FSVRTEGIYTIMITLAIGVAFYYLAQQNYSLFNGFQGFQSVMTPAVAGVELRAPIPFY
                     YLALFWALAGYFAVKYLLRAPFGVALQGIRDNPRRMAA"
     gene            complement(779..1615)
                     /locus_tag="VNU03_02295"
     CDS             complement(779..1615)
                     /locus_tag="VNU03_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73306.1"
                     /translation="MNGLTLAALYFLVASGFTLVFGLMRNVNLAHGSLYLLGAYVGYM
                     VGDRTGSWLMAVAAGFASAALFGLVLQVAVFRFMHGEDLRQTLVTIGLSIMMADILLW
                     IFGGEIYQFDPPRAIYGRTLLPIVEVYPTYRLVLLAMSAAIGVLLWWFLNRTRVGMMI
                     RAGVDDRAMLSAAGVNVQFVFAITFAIGAGLAGFAGVVGGTALSIAPGEDTRYQLASL
                     VVVIVGGMGSVVGAAIGAILIGVAEQFGLAYAPTYSVVFTFVIMALVLAFRPQGILGR
                     RA"
BASE COUNT          378 a          779 c          675 g          250 t
ORIGIN      
        1 gcggccatgc gccgcgggtt gtcgcggatg ccctgcaacg ccaccccgaa gggcgcgcgc
       61 agcaggtact tgacggcgaa gtagccggcg agcgcccaga acagcgcgag atagtagaac
      121 gggatcgggg cgcggagctc cacgcccgcc accgccggcg tcatcacgct ctggaagccc
      181 tggaagccgt tgaagagcga gtagttctgc tgcgcgaggt agtagaaggc caccccgatc
      241 gccagcgtga tcatgatcgt gtagatgccc tcggtgcgca ccgagaacca gccgatgagg
      301 gtggcgcaga tcgtcgcgat cacgagggcc aggaccaccg cgagccccca cggccagcgc
      361 aggctgatgt cggtgccgct cgtggcgaag atcgcgtaca tgtagcccgc gattcccgcc
      421 accgtcatct gggcgagcga gaccatgccc ccgtaaccgg cgagaaacgt cagggagagc
      481 gcgatcagcc cgagcgccag cgcctgggcc ccgatctgga aggtgaagaa cggagtcgcc
      541 acccacgggt aagccagcac cgccagcagc accagcagat gccgcactct cacccgccgc
      601 gcgatccacc ctgcccacgc cacccccccg cccgcaacct ccccctctcc ccccagggga
      661 gagggccggg gtgaggggcc ggtcgcgggc cgcgcggtcg cccgccgccc cgcagctccc
      721 cccgcccacg cgtagcgccc tgcccccgcg ttcatcgcct tccgccagtc cgccgcgctc
      781 acgcccgcct cccgagaatt ccctgcggcc ggaaggcgag cacgagggcc atgatcacga
      841 aggtgaacac cacgctgtac gtcggcgcgt aggcgagccc gaactgctcc gccaccccga
      901 tgagaatcgc cccgatcgcg gccccgacga cgctccccat cccgcccacg atgaccacca
      961 cgagcgaggc gagctggtag cgcgtgtcct cgcccggggc gatcgacagc gcggtgcccc
     1021 cgaccacccc cgcgaagccg gcgaggcccg ccccgatcgc gaaggtgatg gcgaacacga
     1081 actgcacgtt gacgccggcg gcggacagca tggcccggtc gtccaccccg gcgcggatca
     1141 tcatcccgac gcgggtgcgg ttgaggaacc accagagcag gacgccgatg gcggccgaca
     1201 tcgcgagcag caccagccgg taggtcggat agacctccac gatcggcagg agcgtccggc
     1261 cgtagatggc gcgcggcggg tcgaactggt agatctcgcc gccgaagatc cagagcagga
     1321 tgtccgccat catgatcgag agcccgatgg tcaccagggt ctggcgcagg tcctcgccgt
     1381 gcatgaagcg gaacaccgcc acctggagca ccaggccgaa cagggccgcg ctcgcgaatc
     1441 cggccgccac cgccatcagc cacgagccgg tccggtcgcc caccatgtag ccaacgtacg
     1501 cgccgagcag gtagagcgag ccgtgggcca ggttcacatt gcgcatgagg ccgaagacca
     1561 gcgtgaaccc gctcgccacc aggaagtaca gcgccgcgag cgtgagccca ttcatccccg
     1621 tggtcagaaa gagcttcggg ttgtcggcaa gtccccacac cacgaacgcc gccgccggca
     1681 ccccgaccac cagcccccaa aaccaccgcc agaatgttga catgctggct ctagtccacc
     1741 ctgctcatga agcggctacc gtgaccggac gggtggcgcc gcgggcggtt tccgcgcgac
     1801 cgagcgcagt gggccctccg agttcggtgc gtcgctcgaa gagcgacgag tccggcgagt
     1861 gggtgacgga gccggtcgcg cgggagccgc ccgcggcgac aggacccgtc cgcctcaccc
     1921 gtggttcgtt catgaggcgg acccgttaca tgcgcctgga atagaagcgt cgcaacaggt
     1981 catcgcactc ctcatgcttc tcgaccatgg tccagtgccc gcagcgcggc agcaccgtca
     2041 cccgcgcccc ccggatgcgc tcgccgatct gtcgcacgcc ct
//
LOCUS       DATMCV010000093         6322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_204334, whole genome shotgun sequence.
ACCESSION   DATMCV010000093 DATMCV010000000
VERSION     DATMCV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204334"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1860
                     /gene="glgP"
                     /locus_tag="VNU03_02300"
     CDS             247..1860
                     /gene="glgP"
                     /locus_tag="VNU03_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWP73307.1"
                     /translation="MTATSRLPLVAYFSMEYGLHEEFHSYAGGLGVLAGDFMKSAGDL
                     GLPVVGVGLRWSQGYTSQHIGTDGYPYDEWADQKTDVLSDTGARVRVRVKAREVECRV
                     SRVGRYAIAPLYLLEPTDPRDLWITRRLYDPRPDCRVAQEMLLGIGGVRALRALHLPV
                     ERYHFNEGHAVFAGVELIADRMETGRAFREAWRDARRSIVFTTHTPVPAGNEVHAIAE
                     LRRLGAGCELVPGELAEIGGDPFNMTVAGLRLAHMANAVAQLHGETARAMWAHVEDAA
                     PIIAITNGVHAGTWQDARIPAAHGNDERLLAAKRALKGELLAEVRTRTGTRLDPDALT
                     IGFARRSATYKRSDLLLRDPGRFATLIKRHRVQLLFAGKAHPADQEGKAVIARLVEGL
                     RQWPDSVAFLEDYDMGLGRLLTRGCDVWLNTPVRPMEASGTSGMKAAMNGTLNLSVLD
                     GWWPEGCEHGVTGWAIGDGTVSGDPDPRDLRALYDTLETEVLPTFADRGRWTRMMRAS
                     IRMGVERFSSDRMVREYFERLYSVTSPAR"
     gene            complement(1870..3063)
                     /locus_tag="VNU03_02305"
     CDS             complement(1870..3063)
                     /locus_tag="VNU03_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73308.1"
                     /translation="MAGLGIVTVPHPIGGIAEELVARKATAAVEQVQAALIGTVAVGG
                     APGAVTEGAVARHPAPDDLLDFQAWLMERGWGDGLPAIPATSERVNAMLAGTRRPAGD
                     AVAVLSPRLGRATVERVAVNAVLAGALPEHLPVILAAVEAVSQPAFNLQAVQTTTHPC
                     SPLIIVNGPLALRLGVNGGGNALGQGHRANAVIGRALRLVLQNVGGARPGREDRATQG
                     HPGKYSYCVAENEAASPWEPLSVERGFAREISTVTVCGSEAPHNINDHGSSTPEGILT
                     TVAGSVATVGSNNIYLGGEPLVILGPEHAATVAGGGWSKSDFKRALWERAQVPLARFD
                     PSNLERFAVIHPAGFKDRPPETLAPIAREWSDIMIVVAGGAGKHSVFVPTFGATRSVT
                     VRIEE"
     gene            complement(3187..3465)
                     /locus_tag="VNU03_02310"
     CDS             complement(3187..3465)
                     /locus_tag="VNU03_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73309.1"
                     /translation="MEILDPVGPTTATTRTLAPRPASLQGAVIGVLDNSKPNARVLLE
                     GVARALAAKLGAAEVRVWRKPGASIGATRAVLDEMAAHCGAVLTASAD"
     gene            3518..4375
                     /locus_tag="VNU03_02315"
     CDS             3518..4375
                     /locus_tag="VNU03_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HWP73310.1"
                     /translation="MPTPRSLPRHAATRYVQPLREGGSLPAVVDTATGGLFVVKFRGA
                     GQGPKALIAELVVGQLAQALDLPVPELALVELSPAFGRTEADPEIQDILRGSQGVNVG
                     LRYLDGAFNFDATAAGELIAADFATRLVWLDALVTNPDRSHRNPNLLVWRRNPWLIDH
                     GGALYAQHDWAAVDEARTRTPFARIRDHVLLDRAGDLGAIDATLAAALTSDVISRVLD
                     QVPDELFVDPVGGPVGGREFDSAAAARARYREYLAMRLRAPRDFVGEATRAQEQARRA
                     PARPLSARR"
     gene            4372..4791
                     /locus_tag="VNU03_02320"
     CDS             4372..4791
                     /locus_tag="VNU03_02320"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HWP73311.1"
                     /translation="MSAGGAIAYDFAVLRAVPHVHLGAFVPVGVVVHARTAGYLGMRT
                     LVGVDQLRARVHDVDHELLARYLASGCAVAAGDPTAGPVALAPPSERFHWLTSPRSDV
                     LQSSPVHAGLTDDPARALDELFATYVLAPAVGGARER"
     gene            4821..5399
                     /gene="pssA"
                     /locus_tag="VNU03_02325"
     CDS             4821..5399
                     /gene="pssA"
                     /locus_tag="VNU03_02325"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HWP73312.1"
                     /translation="MLRNFHLADLFTIANGFCGVAALFAAMRFLATGDRPHLYWAALL
                     VPVALVFDVLDGRIARWRHVASPMGRELDSLADVISFGVAPAGIAYAAGLDRPADQAI
                     LMYFVGCGVSRLARYNVTAADLSRETGKVAYFEGTPIPTSVVPLGLLMLAFHYRILYP
                     VHVLGLDLHLVSLLFLLSGSLMISKTLRIPKP"
     gene            complement(5569..>6322)
                     /locus_tag="VNU03_02330"
     CDS             complement(5569..>6322)
                     /locus_tag="VNU03_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73313.1"
                     /translation="GFGWAATFLFRAPLFLALAVLTQTQVRRLGGAAPVRRRFALADL
                     LRLPVLRAALLAFLSNHAQFAVWLLVPFFLVGTLGLSPTVGGLVFALTPLANALVAPL
                     GGVITDRVGARGPLACGLVLEVAGLLIIATLTSTTSLVVVGVAMALVGLGVGLFQVPN
                     LALMMAFFPPAQQGVAGGVAFLSRTLGSAVGVQVTAALFAARLGRDGFLPAFHAAFLA
                     AAAVCAVGAVLALLPGGAPGPARAAPGARPGP"
BASE COUNT          831 a         2401 c         2263 g          827 t
ORIGIN      
        1 cctccgcgaa accgtgtctt ctgcgcgccg ccgaaggaaa aacgcgctcc gtctcgtggc
       61 ccgatcgagt gcccacgcgc ccggcgagtc agcgtgtttc gcggagggcc cccggccgcc
      121 tccccccgcc cccctgccgc gccgcgcgcc gattttgcgt tcattgacgt ttgccctatg
      181 ctcgatctgg tgagcttcca tctcgacctg ctgaccgccg ctggtcacta gacgggcacc
      241 tctccgatga ccgcgacgag ccggctcccg ctggtggcgt acttctcgat ggagtacggg
      301 ctgcacgagg agttccactc gtacgcgggc ggcctcgggg tgctcgcggg cgacttcatg
      361 aagtcggccg gggatctcgg gctcccggtg gtgggggtcg ggctccgctg gagccagggc
      421 tacacctcgc agcacatcgg caccgacggc tacccctacg acgagtgggc cgaccagaag
      481 accgacgtgc tctccgacac cggcgcgcgc gtgcgcgtac gggtcaaggc ccgcgaggtc
      541 gagtgccgcg tgtcgcgcgt gggccgctac gccatcgccc cgctctacct gctcgagccc
      601 accgatccgc gcgatctctg gatcacacgg cgcctctacg acccgcggcc cgactgccgg
      661 gtggcccagg agatgctgct cggcatcggc ggggtgcgcg cgctgcgggc gctccacctg
      721 cccgtcgagc gctatcactt caacgagggg cacgcggtct tcgcgggggt cgagctgatc
      781 gcggaccgga tggagaccgg ccgcgccttc cgcgaggcgt ggcgggacgc gcgccgctcg
      841 atcgtcttca ccacccacac cccggtcccc gccggcaacg aggtccacgc catcgccgag
      901 ctgcggcggc tcggcgcggg ctgcgagctg gtgccgggcg agctggccga gatcgggggc
      961 gatccgttca acatgacggt ggcgggcctc cggctcgccc acatggccaa cgcggtcgcc
     1021 cagctccacg gcgagaccgc ccgcgcgatg tgggcccacg tggaggacgc cgcgcccatc
     1081 atcgcgatca ccaacggggt gcacgccggg acctggcagg acgcgcgcat cccggccgcc
     1141 cacggcaacg acgagcggct gctcgcggcc aagcgcgcgc tcaagggcga gctgctcgcg
     1201 gaggtgcgca cgcggaccgg cacgcgcctc gatcccgacg cgctcaccat cggcttcgcc
     1261 cggcggtccg cgacctacaa gcgctccgac ctgctgctgc gcgaccccgg ccgcttcgcg
     1321 accctgatca agcgccaccg cgtccagctc ctgttcgcgg gcaaggcgca tccggccgac
     1381 caggagggca aggcggtcat cgcgcggctc gtcgagggcc tgcgccagtg gcccgacagc
     1441 gtggccttcc tcgaggacta cgacatgggg ctcggccgcc tgctcacccg cggctgcgac
     1501 gtctggctca acaccccggt gcggccgatg gaggccagcg gcacctcggg catgaaggcg
     1561 gccatgaacg gcacgctgaa cctctcggtg ctcgacggct ggtggccgga gggctgcgag
     1621 cacggcgtga ccgggtgggc catcggcgac ggcaccgtga gcggcgatcc cgatccgcgc
     1681 gacctgcgcg cgctctacga caccctcgag accgaggtgc tgcccacctt cgccgatcgg
     1741 ggccgctgga cgcggatgat gcgcgcctcg atccggatgg gggtggagcg cttctcctcg
     1801 gaccggatgg tccgggagta cttcgagcga ctctactcgg tcacgtcccc cgcgcgatag
     1861 cctcggctct cattcctcga tgcggacggt gaccgagcgg gtggcgccga acgtcggcac
     1921 gaacaccgag tgcttccccg ctccacccgc caccacgatc atgatgtccg accactcccg
     1981 cgcgatcggg gccagggtct ccggcggccg gtccttgaag ccggcggggt ggatcaccgc
     2041 gaagcgctcg aggttggagg gatcgaagcg cgcgagcggc acctgggccc gctcccacag
     2101 cgcgcgcttg aagtcgctct tgctccagcc cccgcccgcc accgtggccg cgtgctcggg
     2161 ccccaggatc acgagcggct cgccccccag gtagatgttg ttgctcccca ccgtggccac
     2221 gctgcccgcc accgtggtga ggatgccctc gggcgtcgac gagccgtggt cgttgatgtt
     2281 gtgcggagcc tcggagccgc agaccgtcac cgtgctgatc tcccgcgcga agccccgctc
     2341 gacggagagt ggctcccagg gcgacgcggc ctcgttctcg gccacgcagt agctgtactt
     2401 gcccgggtgg ccctgcgtcg cgcggtcctc ccggccgggc cgcgcgccgc ccacgttctg
     2461 cagcacgaga cgcagggcgc gcccgatcac cgcgttggcg cggtgcccct gccccagcgc
     2521 gttgccgccg ccgttgacgc cgaggcggag cgcgagcgga ccgttgacga tgatgagcgg
     2581 cgagcaggga tgcgtagtgg tctgaaccgc ctggagattg aacgcgggct ggctcaccgc
     2641 ctcgaccgcg gcgaggatca ccggcaggtg ctcgggcagc gcccccgcca gcaccgcgtt
     2701 caccgccacc cgttccacgg tggcgcgacc gagccgcggc gacaacaccg cgaccgcgtc
     2761 gcccgcgggg cgccgagtgc ccgcgagcat cgcgtttacg cgctcggagg tggccggaat
     2821 cgcgggcagc ccgtcgcccc agccccgctc catcagccag gcctggaagt cgagcagatc
     2881 gtccggggcg ggatggcgcg ccaccgctcc ttcggtcacc gctcccggcg cgccgcccac
     2941 cgcgacggtg ccgatgaggg cggcctgcac ctgctcgacc gcggcggtcg ccttccgcgc
     3001 gaccagctcc tccgcgatgc ccccgatggg gtgcggcacc gtcacgatgc cgagcccggc
     3061 catccccttg gcgcgcgcct cggccagggc gagcgggcgg aactcgtgcg tgcccaggac
     3121 gacggtcgcg atcccgcgcc cttccagctc cgtggcgtcg tggatactcc acgacgtgca
     3181 cgaccctcag tcggcggagg cggtgaggac ggccccgcag tgggccgcca tctcgtcgag
     3241 gaccgcgcgg gttgccccga tggaggcgcc gggcttgcgc cagacccgca cctccgcggc
     3301 gccgagcttc gccgcgagcg cgcgggccac gccttccagc aggacgcgcg cgttgggctt
     3361 ggagttgtcg agcacgccga tgaccgcgcc ctgcagcgaa gccgggcggg gcgcgagcgt
     3421 gcgcgtggtg gcggtggtcg ggccgaccgg gtcgaggatc tccatgaccc gcatcatacc
     3481 gcgccccccg gcccgtgtag tacagtcctg ggcggagatg cccacaccac gatcgctgcc
     3541 gcgccacgcg gcgacccgct acgtgcagcc gctccgggag ggcggctcgc tgcccgcggt
     3601 ggtcgacacc gcgaccggag gcctcttcgt ggtcaagttc cggggcgccg ggcagggccc
     3661 gaaggcgctg atcgccgagc tggtggtggg ccaactcgcc caggcgctcg acctaccggt
     3721 gccggagctc gcgctcgtgg agctctcccc cgccttcggc cggaccgagg ccgatcccga
     3781 gatccaggac atcctgcgcg gcagccaggg ggtgaacgtg gggctgcgct acctcgacgg
     3841 ggcgttcaac ttcgacgcca ccgcggccgg cgagctgatc gccgccgact tcgccacccg
     3901 gctcgtgtgg ctcgacgcgc tcgtcaccaa cccggaccgg agccatcgca accccaacct
     3961 gctcgtgtgg cgccggaacc cgtggctcat cgatcacggc ggcgcgctct acgcgcagca
     4021 cgactgggcg gcggtggacg aggcgcggac ccgcaccccg ttcgcccgca tccgcgacca
     4081 cgtgctgctc gatcgggccg gcgacctggg cgcgatcgac gcgacgctgg ccgccgcgct
     4141 cacctccgac gtgatcagcc gcgtgctcga ccaggtgccc gacgagctgt tcgtcgatcc
     4201 ggtgggtggg ccggtgggcg ggcgcgagtt cgactccgcg gcggcggccc gcgcccgcta
     4261 tcgcgaatac ctcgcgatgc gcctgcgcgc cccgcgcgac ttcgtgggcg aggcgacccg
     4321 cgcgcaggaa caggcccgtc gcgcgccggc gcgcccgctc tcggcccgcc gatgagcgcg
     4381 gggggcgcga tcgcgtacga cttcgcggtg ctccgcgcgg tgccccacgt gcacctgggg
     4441 gcgttcgtgc cggtgggggt cgtcgtccac gcgcgcaccg cgggttacct cgggatgcgc
     4501 accctggtcg gcgtcgacca gctgcgcgcg cgcgtccacg acgtcgacca cgagctgctc
     4561 gcccgctacc tggcctccgg ctgcgcggtc gcggcgggcg atcccaccgc ggggccagtc
     4621 gcgctcgcgc ccccgtcgga gcgcttccac tggctcacct ccccgcgctc ggacgtgctc
     4681 cagagctcgc cggtgcacgc gggcctcacc gacgatccgg cccgggccct cgacgagctg
     4741 ttcgcgacct acgtgctggc ccccgcggtg ggcggggcgc gggagcgcta gcgttggccg
     4801 cgccgcgacg gcacttctcg atgctgcgga acttccacct cgccgatctc ttcacgatcg
     4861 ccaacggctt ctgcggcgtc gcggccctct tcgcggcgat gcggttcctc gccaccggcg
     4921 accgtccaca cctctactgg gccgcgctgc tggtcccggt ggccctggta ttcgacgtcc
     4981 tcgacggccg catcgcgcgg tggcggcacg tggcctcgcc gatgggccgc gagctcgact
     5041 cgctggccga cgtgatctcg ttcggggtgg cgccggccgg catcgcctac gcggccgggc
     5101 tcgaccgccc cgcggaccag gcgatcctga tgtacttcgt ggggtgcggg gtgagccggc
     5161 tcgcgcgcta caacgtcacc gccgccgatc tctcccgcga gaccggcaag gtcgcctact
     5221 tcgagggcac cccgatcccg accagcgtgg tgcccctggg cctgctgatg ctggccttcc
     5281 actaccgcat cctctacccg gtgcacgtgc tcgggctcga cctccacctg gtctcgctgc
     5341 tcttcctgct ctcgggcagc ctgatgatca gcaagacgct ccggatcccg aagccctgac
     5401 ccccccgcgg tcgcccgctc tcccgcgccg ccgcgtagct caatcccatg tccgcgatga
     5461 acgtggggat cagcggggcg aagccgacgc ggtggacgtg ggtctgcatg aacccgagca
     5521 cgcacaccgc gaggatcgcc cagcggtagg gcgaggcctc ggcggtcgtc acgggcccgg
     5581 gcgcgccccc ggcgccgcgc gcgcggggcc gggggcgccg ccgggcagca gcgcgagcac
     5641 cgcgccgacc gcgcataccg cggcggccgc gaggaacgcg gcgtggaacg cgggcaggaa
     5701 gccgtcgcgg ccgaggcgcg cggcgaagag cgcggcggtc acctgcacgc ccaccgcgct
     5761 gcccagcgtg cggctcagga aggcgacgcc gcccgccacg ccctgctggg cgggcggaaa
     5821 gaacgccatc atcagggcca ggttcggcac ctggaagagc ccgacgccca ggccgacgag
     5881 cgccatcgcg acgccgacca cgacgagcga ggtggtggac gtcagcgtgg cgatgatcag
     5941 gaggcccgcc acctcgagca cgaggccgca cgcgagcggc ccgcgcgcgc cgacgcggtc
     6001 ggtgatgacg ccgccgagcg gggccaccag cgcgttggcg agcggggtga gggcgaacac
     6061 gagcccgccc acggtggggg agagaccgag cgtgccgacc aggaagaacg gcaccagcag
     6121 ccacaccgcg aactgggcgt ggttcgacag gaacgcgagc agcgcggccc gcagaaccgg
     6181 cagccggagc aggtccgcga gcgcgaagcg acgcctcacc ggcgcggcgc cgcccagccg
     6241 acggacctgc gtctgggtca gcacggccag agccaggaag agcggggcgc ggaagaggaa
     6301 ggtcgcggcc cagccgaagc cg
//
LOCUS       DATMCV010000094         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_139313, whole genome shotgun sequence.
ACCESSION   DATMCV010000094 DATMCV010000000
VERSION     DATMCV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139313"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VNU03_02335"
     CDS             <1..1214
                     /locus_tag="VNU03_02335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWP73314.1"
                     /translation="LQGDVGSGKTVVAALGILTAVESGYQTALMAPTEILAEQHLMTL
                     TQLLEPLAVEVALLTNAVKGKARERVGAGTADGSIKCVVGTHALVQGDVRFRRLGLAV
                     VDEQHRFGVNQRATLRGKGESPDVLVMTATPIPRTLALTLYGDLEVSVIDELPPGRKP
                     VVTKARTESARRQIYGFLREQVSEGRQVYVVYPLVEESEALDLRAATEMAARLQGEVF
                     ADLRIGLLHGRMAFAEKDRVMREFKAGAIHILVSTTVIEVGIDVPNASVMLIEHAERF
                     GLSQLHQLRGRVGRGPWKSYCILLSGAASEDARRRLDAMTATGDGFKIAEADLSLRGP
                     GDFFGTRQSGLPEFRVADLLRDAAALEAARRDAVALIRDDPQLLAPEHRALRTALLQR
                     WRGKLDLAGVG"
     gene            1214..1657
                     /locus_tag="VNU03_02340"
     CDS             1214..1657
                     /locus_tag="VNU03_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73315.1"
                     /translation="MRPRRWGALGCALALGVGAAPAPARAQDSAELARARQVLEYYQA
                     CERTRRFAPCWSLLSTRAQEAWSRQGRGTVDEYAEAKGAAEPRFSDFRVLRIRRSPAR
                     VVFVVEASGGTEKSAIWERVEYALLRESGQWKVDGRRVGASETTP"
     gene            1698..>2170
                     /gene="rsmD"
                     /locus_tag="VNU03_02345"
     CDS             1698..>2170
                     /gene="rsmD"
                     /locus_tag="VNU03_02345"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWP73316.1"
                     /translation="MRVLAGALKGQRLTTPRGRTTRPTADQVRIACLDTLMPYLERGP
                     FLDLFAGAGGVGIEALSRGAPSATFVEQDGAALRALRDNVERLGLGERARVVRADAAR
                     AVADLAAGGDRFAVVFLDPPYEAPRAAAALDAVAVGDILLPGAVVVLQHRTKAPPP"
BASE COUNT          272 a          782 c          823 g          293 t
ORIGIN      
        1 tcctgcaggg cgacgtgggc tcgggcaaga cggtggtggc cgcgctcggg atcctcaccg
       61 cggtggagtc gggttaccag accgcgctga tggcccccac cgagatcctg gccgagcagc
      121 acctgatgac tctgacccag ctcctcgagc ccctcgcggt ggaggtggcg ctcctcacca
      181 acgcggtgaa gggcaaggcc cgcgagcggg tgggcgcggg gaccgcggac gggagcatca
      241 agtgcgtggt gggcacccac gcgctcgtgc agggcgacgt ccgcttccgg cgtctcggtc
      301 tcgcggtggt ggacgagcag catcgcttcg gggtgaacca gcgcgcgacc ctccggggca
      361 agggggagag cccggacgtg ctcgtgatga ccgccacccc gatcccgcgc acgctcgcgc
      421 tcaccctcta cggcgacctc gaggtctcgg tcatcgatga gctgccgccg ggccgaaagc
      481 cggtggtcac caaggcccgc accgagtcgg cccgccggca gatctacggc ttcctgcgcg
      541 agcaggtgag cgaggggcgc caggtgtacg tcgtctatcc tctggtggaa gagtccgagg
      601 cgctcgacct tcgcgcggcc accgagatgg ccgcgcggct gcagggggag gtcttcgccg
      661 atctccggat cggcctgctc cacgggcgca tggccttcgc ggagaaggac cgggtcatgc
      721 gcgagttcaa ggcgggcgcc atccacatcc tggtctcgac caccgtgatc gaggtgggca
      781 tcgacgtgcc caacgcctcg gtgatgctca tcgagcacgc ggagcgcttc ggcctctccc
      841 agctccacca gctccgcggc cgggtcgggc gcggcccgtg gaagagctac tgcatcctgc
      901 tgtcgggggc cgcctccgag gacgcccggc ggcgcctcga cgcgatgacc gccaccggcg
      961 acggcttcaa gatcgcggag gcggatctct cgctgcgcgg gccgggcgac ttcttcggta
     1021 cccgccagtc ggggctgccc gagttccgcg tcgcagacct gctgcgcgac gcggccgcgc
     1081 tcgaggccgc gcgccgcgac gcggtggcct tgatccgcga cgatccgcag ctcctcgccc
     1141 ccgagcaccg cgcgctgcgg accgcgctgc tccagcgctg gcgcggcaag ctcgacctgg
     1201 ccggcgtcgg ctgatgcgac cccgccgctg gggcgcgctg ggatgcgcgc tcgccctcgg
     1261 ggtgggcgcc gcccccgcgc ccgcccgcgc gcaggacagc gccgagctgg cccgcgcccg
     1321 acaggtgctc gagtactacc aggcgtgcga gcgcacgcgc cgcttcgcac cgtgctggag
     1381 cctgctctcc acgcgggcgc aggaggcgtg gagccggcag gggcggggga ccgtcgacga
     1441 gtacgcggag gccaagggcg cggccgagcc acgcttctcg gatttccgcg tgctccggat
     1501 ccggcggagc cccgcgcggg tggtgttcgt ggtggaagcg agcggcggca ccgagaagag
     1561 tgcgatatgg gagcgcgtgg agtacgccct gctccgcgag agcgggcagt ggaaggtgga
     1621 cggccggcgg gtcggggcct ccgagaccac gccgtgaacg cccctcaccc tgccctctcc
     1681 ccgatgggga gagggtgatg cgcgtgctcg cgggggcgct caagggccag cgtctcacca
     1741 cgccgcgcgg ccgtaccacg cgcccgaccg cggaccaggt tcgcattgcg tgcctcgaca
     1801 ccctgatgcc gtatctcgag cgcggcccct ttctcgacct gttcgcgggc gcgggcgggg
     1861 tcggcatcga ggccctctcg cggggcgccc cgtccgccac cttcgtcgag caggacggcg
     1921 ccgcgctgcg ggcgctgcgc gacaacgtcg agcgactggg cctgggggag cgtgcgcgcg
     1981 tggtccgcgc ggacgccgcg cgcgcagtgg cggacctcgc cgcggggggc gatcggttcg
     2041 cggtcgtgtt cctcgatccg ccctatgagg ccccgcgcgc ggcggccgcg ctcgacgcgg
     2101 tggcagtggg cgacatcctg ctgccgggcg cggtggtcgt actccagcat cggacgaagg
     2161 cgccgccgcc
//
LOCUS       DATMCV010000095         3389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_204479, whole genome shotgun sequence.
ACCESSION   DATMCV010000095 DATMCV010000000
VERSION     DATMCV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204479"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VNU03_02350"
     CDS             <1..1506
                     /locus_tag="VNU03_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73317.1"
                     /translation="VKHPAYRRMEEIAFGRFGLAAMAHRPGVLGFPERAPQVVKYALS
                     YLFAQSEFGLLCPVNMTDSCARMLGAFGSEELKARYLPHLTATDPAALWQGTQWMTER
                     TGGSDVGASTTVARRDADGQWRLWGDKWFTSVANAELALTLARPEGAPAGTRGLAMFL
                     VPKHLPDGGKNAWTINRLKDKLGSRSMATGEVTYAGARAWVVGDPGAGFKQMMEMVNG
                     SRLSNAMRAAGLMRRGLLEALVHARQRVAFGHPLIELPLLRSNLLSMLLDAEAAASVI
                     LNGAVLLDRRDAGSAEARALARVWTPLAKYWITLRARDVTAEAMNVRGGNGYVEEWVN
                     PRLLRDAYLGAIWEGSANVVALDVQRAILKDRGLEVLAAFIGERLGRVAEPAAKPLVD
                     LARATLETVARQTAGWAARPAAERELAARPVADTLYHLLAGSLLLAEGQALREQGHGF
                     RKLLSAGLYLKRWLEPRGPASPPFTARQVEWLEPLAGWRAIEESALADLEA"
     gene            1513..>3389
                     /locus_tag="VNU03_02355"
     CDS             1513..>3389
                     /locus_tag="VNU03_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP73318.1"
                     /translation="MATDEIIWRPDPAAARTRMGRFMAAHGLATLADLQRRSVADPEW
                     YWDAVSRDLGFHWFTPYRRVLDTSKGIEWPRWFEGGRLNLADNSVDRHIAAGRGDKLA
                     VIAEAEDGTVRTLSYRELAAEVGRLANAIKRLGIRPGDTVGILLPMCAEAVIATLAVV
                     RIGAVYTPCFSGYGAQAVASRLDGCGARLLITADGFARRGQPIPLKRTADEAVSACPS
                     VEHVIVHRRSGVEVPWKDGRDLWWDDLVAVESAECPALPVEADHPCLIIYTSGTTGRP
                     KGTVLTHGGFSLKNAHDFAYLFDLGERDRLFWVTDLGWLMGPMLIGGALLTGGTAVLF
                     EGTPDYPKPDRLWSTLERHRVTVLGISPTAVRALMPHGAEWVTRHDLSSLKLMASTGE
                     PWNPEPYRWLFETAGKGRLPIINYTGGTEISGGILSCFPIAPIKPCSFAGPIPGMAAD
                     VYDDDGRPVRGQVGELVVTRPWPGMTAGFWKDPERYLETYWSRWPGVWVHGDWAIVDE
                     DGFWFIQGRSDDTLKIAGKRVGPAEVESVLVGHPAVAEAAAVGVPHEIKGETVVCFAV
                     LRPGFAPSEPLRVELVEEVVRSMGKAIRPERVLFARELPKTRSAKIMRRVVRATYLGR
                     DP"
BASE COUNT          434 a         1198 c         1290 g          467 t
ORIGIN      
        1 gtgaagcatc ccgcgtatcg ccggatggag gagatcgcgt tcggccgctt cgggctcgcc
       61 gcgatggcgc accggcccgg cgtgctcggc ttccctgagc gcgcgccgca ggtggtgaag
      121 tacgcgctct cgtacctctt cgcgcagagc gagttcggcc tgctctgccc ggtcaacatg
      181 accgactcct gcgcgcgcat gctgggggcc ttcggcagcg aggagctcaa ggcgcgctac
      241 ctgccgcacc tcaccgccac cgatccggcc gcgctctggc agggcaccca gtggatgacc
      301 gagcgcaccg gcggctccga cgtcggcgcc tccaccacgg tggcccggcg cgacgcggac
      361 gggcagtggc ggctctgggg cgacaagtgg ttcacctcgg tggccaacgc ggagctggcc
      421 ctgaccctcg cccggcccga gggggcgccc gcgggcacgc gcgggctcgc gatgttcctc
      481 gtccccaagc acctgccgga cggcggcaag aacgcgtgga cgatcaaccg gctgaaggac
      541 aagctcggct cgcgctcgat ggccaccggc gaggtcacct acgccggcgc gcgggcctgg
      601 gtggtgggcg atccgggtgc gggcttcaag cagatgatgg agatggtgaa cggctcgcgg
      661 ctgtcgaacg cgatgcgggc ggccgggctc atgcggcgcg ggctgctcga ggcgctcgtg
      721 cacgcgcggc agcgggtcgc cttcgggcac ccgctgatcg agctgccgct cctgcgcagc
      781 aacctgctct cgatgctgct cgacgcggag gccgccgcct cggtcatcct gaacggcgcc
      841 gtgctgctcg accgccgcga cgcggggagc gcggaggcgc gcgcgctcgc gcgggtgtgg
      901 accccgctcg ccaagtactg gatcaccctg cgcgcgcgcg acgtgaccgc ggaggcgatg
      961 aacgtgcgcg gcggcaacgg ctacgtcgag gagtgggtca acccgcgcct gctgcgcgac
     1021 gcctacctgg gcgccatctg ggagggctcg gccaacgtgg tggcgctgga cgtgcagcgc
     1081 gcgatcctga aggaccgcgg gctcgaggtg ctggccgcgt tcatcggcga gcgcctcgga
     1141 cgggtcgcgg agccggcggc caagccgctg gtggacctgg cccgcgcgac cctggagacg
     1201 gtcgcgcgcc agaccgcggg ctgggccgcg cggcccgccg cggagagaga gctggccgcg
     1261 cggccggtgg ccgacacgct ctaccacctg ctggccggct cgctcctcct ggccgaaggc
     1321 caggcgctgc gcgagcaggg gcacggcttc cggaagctgc tctcggccgg gctctacctc
     1381 aagcggtggc tcgagccgcg cggccccgcg agcccgccct tcaccgcgcg ccaggtcgag
     1441 tggctcgagc cgctggccgg gtggcgggcc atcgaggagt cggcgctggc cgacctggag
     1501 gcctgaggcg cgatggcgac cgacgagatc atctggcgac ccgatccggc ggcggcgcgc
     1561 acccgcatgg gccgcttcat ggccgcgcac gggctcgcga cgctcgccga cctgcagcgg
     1621 cgctcggtgg cggacccgga gtggtactgg gacgcggtct cgcgcgacct gggcttccac
     1681 tggttcacgc cctaccgtcg cgtgctcgac acctcgaagg gcatcgagtg gccccgctgg
     1741 ttcgagggcg gccggctgaa cctggccgac aactcggtgg accgccacat cgcggccggg
     1801 cgcggggaca agctcgcggt catcgcggag gccgaggacg gcacggtccg caccctcagc
     1861 tatcgcgagc tggccgcgga ggtcggccgg ctcgccaacg cgatcaagcg tctcggcatc
     1921 cggccgggcg acaccgtcgg catcctgctg ccgatgtgcg cggaggcggt gatcgccacg
     1981 ctcgcggtgg tgcgcatcgg cgcggtctac accccgtgct tctccggcta cggcgcgcag
     2041 gcggtggcct cgcggctcga cggctgcggg gcgcgcctgc tgatcaccgc ggacggcttc
     2101 gcccggcgcg gccagccgat cccgctcaag cgcaccgccg acgaggcggt gtccgcctgc
     2161 ccgagcgtgg agcacgtcat cgtgcaccgg cggagcgggg tggaggtgcc gtggaaggac
     2221 ggacgcgacc tctggtggga cgacctggtg gcggtggagt cggccgagtg cccggccctg
     2281 ccggtggagg ccgatcaccc gtgcctgatc atctacacct cgggcaccac cgggcggccc
     2341 aagggcaccg tgctcaccca cggcgggttc tcgctcaaga acgcgcacga cttcgcgtat
     2401 ctcttcgacc tcggcgagag agaccggctc ttctgggtca ccgatctcgg ctggctgatg
     2461 gggccgatgc tgatcggcgg cgcgctcctg accggcggca ccgcggtgct cttcgagggc
     2521 acgcccgact acccgaagcc cgatcgcctg tggtccacgc tcgagcggca ccgggtcacc
     2581 gtgctcggca tttcccccac cgcggtgcgc gcgctgatgc cgcacggcgc ggagtgggtg
     2641 acgcgccacg atctctcgtc gctcaagctc atggcctcca ccggcgagcc gtggaacccg
     2701 gagccctatc gctggctctt cgagacggcg gggaaggggc ggctgccgat catcaactac
     2761 accggcggca ccgagatctc ggggggcatc ctgtcgtgct tcccgatcgc gccgatcaag
     2821 ccctgctcgt tcgcggggcc catcccgggc atggccgccg acgtctacga cgacgacggc
     2881 cggccggtgc gcggccaggt gggcgagctg gtcgtgaccc ggccctggcc cggcatgacc
     2941 gcgggcttct ggaaggatcc ggagcgctac ctcgagacct actggtcgcg ctggccgggg
     3001 gtgtgggtgc acggcgactg ggcgatcgtc gacgaggatg gattctggtt catccagggc
     3061 cgctccgacg acacgctgaa gatcgcgggc aagcgcgtgg ggcccgcgga ggtggagtcg
     3121 gtgctggtgg ggcacccggc ggtcgcggag gcggccgcag tcggcgtgcc gcacgagatc
     3181 aagggggaga cggtggtgtg cttcgcggtg ctgcggcccg gcttcgcgcc gtcggagccg
     3241 ctccgcgtcg agctggtgga ggaggtcgtg cgctcgatgg gcaaggcgat ccggccggag
     3301 cgcgtgctct tcgcgcgcga gctgccgaag acccgcagcg ccaagatcat gcgccgcgtg
     3361 gtgcgcgcga cctacctcgg gcgcgaccc
//
LOCUS       DATMCV010000096         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_937651, whole genome shotgun sequence.
ACCESSION   DATMCV010000096 DATMCV010000000
VERSION     DATMCV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937651"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VNU03_02360"
     CDS             complement(<1..1255)
                     /locus_tag="VNU03_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWP73319.1"
                     /translation="MFRTKIFAMVAALVCTSPILASAQSDDSAGVVTTVNGDATLLRA
                     VAAAQPVSLRMRDEIFVRDRIQTRERSLVRVLLGGKALITVRELSVLTVTEEAGRVTV
                     DLQSGKVGVAVVKARMRPGEIIEIRSPNATAAVRGTVFVVEVDPMQPGQAGSAPATTT
                     RVHLFHGALDVSARLDPAQPTVRLAELQSVVVAGNSLGSVKPISRDAVATLTADLKPR
                     QVRTPDAPSEFTGGLVTREQSRAVTLATALLAPTPTAPVQGTLKNVRGTVNGVTGAVT
                     NVTGTTAETLDRLMDSLGLDVLGDTLGNTVGSLGNAVNEVGGTVNGLVSSLGGTVGDL
                     GNVVDGTVAGLGGTVGGLGGTVGGLGGTVGGTVGSLGGTVGGLGGTVGGLGGTVGGLG
                     GTVGGLGSTVGGLGGTVGGLGGTIGG"
     gene            complement(1519..2547)
                     /locus_tag="VNU03_02365"
     CDS             complement(1519..2547)
                     /locus_tag="VNU03_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWP73320.1"
                     /translation="MAALPLIPSTVVGSHGKPGWWFTAVKAYETGAMGPSDLEEMFDD
                     AADTAIRDMDRAGLDIITDGEVRRLDGYVDSYYAIIKGIEPLPVRRKAGPWGYDQQTR
                     YEATGRIETPPGGLGIVKEFEYLKAHTGRGTKATCAGPLTFGSRIHPGKMYKGVVDVA
                     ERFAEVINAELRALVEAGADFIQLDEPARGNVSGEEMARLYNLATEGVKAKLAFHICF
                     GNRFGRSRFDRSYRPYFPGILKCRADQLVLEFAGREFSELDLWKEYGQDRELGAGVVD
                     VKGFYPESPEEVASRIGRVLTVCRPEKLYVNPDCGFGWSPRYMCNQKVRTLAAGAALA
                     RAQLTGSR"
BASE COUNT          378 a          951 c          903 g          397 t
ORIGIN      
        1 ggccgccgat cgtgccgccg agcccgccga cggtgccgcc caggccgccc accgtactac
       61 cgagcccacc gacggtgccg ccgagcccgc cgaccgtacc gcccaggccg cccaccgtgc
      121 caccgaggcc gccgacggtg ccgccgaggc tgccgaccgt cccgccgacg gtgccgccga
      181 gcccaccaac cgtgccgccg aggccgccga ccgtcccgcc gaggccggct acggtgccat
      241 cgacgacatt gccgagatcc ccgaccgtgc caccgaggct actgaccagc ccgttgaccg
      301 tccctccgac ctcattcacc gcgttgccga gggacccgac cgtattcccc agggtgtccc
      361 cgaggacgtc gaggccaagg ctgtccatca ggcggtcgag cgtttccgcg gtcgtgccgg
      421 ttacattggt caccgcgccg gtcaccccgt tcacggtgcc ccgcacgttc ttcagcgtac
      481 cttgcaccgg agcggtcggc gtcggggcca gcagcgccgt cgccagggtc accgcccggc
      541 tttgctcgcg ggttaccagc ccgcccgtga actcgctggg agcgtccggg gtccgtacct
      601 ggcggggctt caggtccgcg gtcagcgtgg ccaccgcatc tctcgagatc ggcttcaccg
      661 agcccagcga gttgccggcc accaccacac tctggagctc ggcgaggcgg accgtcggct
      721 gggcgggatc gaggcgggcg ctcacgtcga gcgcgccgtg gaagaggtgc acgcgggtcg
      781 tcgtcgcggg cgcgctcccc gcctgaccgg gctgcatcgg gtccacctcg accacgaaga
      841 cggtgccgcg caccgcggcc gtcgcgttcg gcgagcggat ctcgatgatc tcgcccgggc
      901 gcatgcgtgc cttcaccacc gcgacgccca ccttgccgga ctgcagatcc acggtgaccc
      961 ggccggcctc ctcggtgacg gtgaggaccg acagctcgcg caccgtgatg agggccttgc
     1021 ccccgaggag cacgcggacc agggagcgct cgcgggtctg gatccgatcc cgcacgaaga
     1081 tctcgtcccg catgcgcaga gagaccggct gggcggcggc caccgcgcga agcagggtcg
     1141 cgtcgccgtt cacggtggtg acgactccgg cactgtcatc ggactgagca gacgcgagga
     1201 tcgggctcgt gcagaccagc gcggccacca tcgcgaagat tttcgtgcgg aacatgatcg
     1261 gtctcgctcc ctttttgccg ggcggttgga tgagcctgtt gcccggcggg caagtgtccg
     1321 agcaaggcgg gtgccacgcg gacactgcgg ccaagtggct ggattccttg tggctagtgt
     1381 gtcagcgact tgacgaacgg gagctgacac tcgcaagaag tggcagcgac agggacaaga
     1441 tcagagtgca gttcgtgctc tcgtgccgcg tgcatgacgc cgcgcccggc cgcgtggcgc
     1501 catgcccgcg ggcaattact agcgagagcc ggtgagctgg gcgcgagcga gggccgcgcc
     1561 ggccgcgagg gtccgcacct tctggttgca catgtagcgc ggcgaccacc cgaagccgca
     1621 gtccgggttg acgtagagct tctccggccg gcacaccgtg agcacgcggc caatgcggct
     1681 tgccacctcc tccgggctct ccgggtagaa gcccttgacg tcgaccaccc ccgcgcccag
     1741 ctcccgatcc tgaccgtact ccttccagag atcgagctcg gagaattcgc ggcccgcgaa
     1801 ctcgagcacg agctggtcgg cccggcactt gaggatgccc gggaagtagg gccggtacga
     1861 gcggtcgaag cgggaccggc cgaaacggtt gccgaagcag atgtggaagg cgagcttggc
     1921 cttcaccccc tcggtggcca ggttgtagag ccgcgccatc tcttccccgc tcacgttgcc
     1981 gcgcgccggc tcgtcgagct ggatgaagtc ggcgcccgcc tccacgaggg cgcgcagctc
     2041 cgcgttgatc acctccgcga agcgctccgc cacgtcgacc acgcccttgt acatcttgcc
     2101 ggggtggatg cgcgagccga aggtgagggg acccgcgcag gtggccttgg tgccgcgccc
     2161 ggtgtgggcc ttcaggtact cgaactcctt cacgatcccg agcccgcccg gcggcgtctc
     2221 gatgcggccg gtggcctcgt agcgggtctg ctggtcgtag ccccagggcc cggccttgcg
     2281 gcgcaccggc agcggctcga tgcccttgat gatggcgtag tacgagtcga cgtagccgtc
     2341 gaggcgtcgc acctcgccgt cggtgatgat gtcgaggccg gcccgatcca tgtcccggat
     2401 cgcggtgtcg gccgcgtcgt cgaacatctc ctcgaggtcg gagggcccca tggcccccgt
     2461 ctcgtaggcc ttcaccgcgg tgaaccacca gcccggcttg ccgtgcgagc cgacgacggt
     2521 ggaggggatc aacgggagag cggccatctg cctgtcctcc tgggctacgc gctgtagtgt
     2581 ccggtcccgt gccggcccgg acccaggaaa gcatcctatc tccggccgg
//
LOCUS       DATMCV010000097         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_406357, whole genome shotgun sequence.
ACCESSION   DATMCV010000097 DATMCV010000000
VERSION     DATMCV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406357"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..405
                     /locus_tag="VNU03_02370"
     CDS             193..405
                     /locus_tag="VNU03_02370"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HWP73321.1"
                     /translation="MEQREWDGMEAAIVEAETALEACRRAAEDPTIASDPAALQARYA
                     AAEAARAEVDRLYARWAELEAKGETR"
     gene            402..701
                     /locus_tag="VNU03_02375"
     CDS             402..701
                     /locus_tag="VNU03_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HWP73322.1"
                     /translation="MSETQAVATVKIDNAQVRVTEWRFAPGAHTGHHRHEYAYVVVPL
                     VTGALAASGAAGETTNALRVGEPYYRDAGVEHDVRNPNPFEFAFIEIELKGAPGA"
     gene            833..973
                     /locus_tag="VNU03_02380"
     CDS             833..973
                     /locus_tag="VNU03_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73323.1"
                     /translation="MKKAPGVFALLAAAVVGFGQHVASLCATAQGRLLRRQGGSRRRQ
                     GR"
     gene            970..1260
                     /locus_tag="VNU03_02385"
     CDS             970..1260
                     /locus_tag="VNU03_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73324.1"
                     /translation="MSAMQKDAAVYTVGDFVRRVVESLFRADFRGRLLCSRCLIKLTK
                     DNLDKSYAKRDIIQVIDELFVTPGSTLQVSTAPCGQCARKKAPCIGMPAEGH"
     gene            complement(1469..1690)
                     /locus_tag="VNU03_02390"
     CDS             complement(1469..1690)
                     /locus_tag="VNU03_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73325.1"
                     /translation="MSRMPSKSFRCEVVSEMVSITLRRRNALVGRGKLFVRCSEKDCQ
                     YLDANEPPCPLTLAVFTAEIRERMIADRD"
     gene            complement(1825..>2350)
                     /locus_tag="VNU03_02395"
     CDS             complement(1825..>2350)
                     /locus_tag="VNU03_02395"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWP73326.1"
                     /translation="PVAGSSGQARRREAPVVVGGDRPVFERCRPIFDAIARRAYHVGG
                     NGAGARAKLVVNLVLGLNRLALAEGLLFGLRQGLDGSDLLAVLKDSAAYSRAMDVKGE
                     RMLTDRFEPEGKLAQHLKDVELMLEVGHAAGAPLLATALHRQLLVAGVAGGWGERDNS
                     SIIAVLRSLVTPTR"
BASE COUNT          353 a          822 c          865 g          310 t
ORIGIN      
        1 gttggtcctg gagaccccgg gcggtggtgg ctacggcgat ccagcggagc gtctggaggc
       61 ggccagggac gaggatcgga agaacggata tacggggtag gcggcttccc cccgggaccc
      121 ctcaccctgg gacccctcac cctaccctct ccccggaggg gagaggggga gaaggggaag
      181 cggcgggggt acctggagca gcgggagtgg gacgggatgg aggcggcgat cgtcgaggcc
      241 gagaccgcgc tcgaggcgtg ccggcgggcc gcggaggatc cgacgatcgc ctcggacccc
      301 gccgcgctcc aggcccgcta cgccgcggcc gaggcggcgc gggcggaggt cgatcggctc
      361 tacgcgcgct gggcggagct cgaagccaag ggagagacgc gatgagcgag acgcaggcgg
      421 tggccaccgt gaagatcgac aacgcgcagg tccgggtgac cgagtggcgc ttcgcgcccg
      481 gggcgcacac cggccatcac cggcacgagt acgcctacgt ggtggtgccg ctggtgaccg
      541 gcgcgctcgc cgcgagcggt gccgccgggg agaccaccaa cgcgcttcgc gtcggcgagc
      601 cctactatcg cgacgcgggg gtcgagcacg acgtgcgcaa cccgaatccc ttcgaattcg
      661 cgttcatcga gatcgagctg aagggcgcgc cgggcgcctg aactccgccg cgaggggcgc
      721 gcaccggcgt gctatcgtag ggcctgcctt ccattgggcc ccttagggtg gcgctgcggg
      781 cgccgaggcc caccggcatc tggctccatt gccctcattc cgaaggagca ccatgaagaa
      841 ggcccccgga gtttttgccc tcctcgccgc cgcggtggtc ggattcggcc agcacgtcgc
      901 gagcctctgt gccaccgccc agggacgtct cctccgtcgg cagggaggaa gtcgccgccg
      961 tcagggccgg tgagcgccat gcagaaggac gccgcggtct acaccgtggg tgacttcgtc
     1021 cgtcgcgtcg tcgagagcct gttccgggcc gacttccggg gccggctgct ctgctcgcgc
     1081 tgcctcatca agctgacgaa ggacaacctc gacaagagct acgccaagcg cgacatcatc
     1141 caggtgatcg atgagctgtt cgtcaccccg ggttccaccc tgcaggtgtc gaccgcgccg
     1201 tgcgggcagt gcgctcggaa gaaggcgccg tgcatcggga tgccggccga gggccattga
     1261 tgtgcgtgta ctgcgtggag gacgcggcct gcgagcacga gcgtgacgcc tgcgcggcga
     1321 gcgggggcca gccctgcacc cagtgcggtc acacctccgc ggcgcggccc tggcagcctc
     1381 ccgcgtttcc cggccccggc ccgcgctgga gccgaccgaa gccgcggccg gcccggagac
     1441 cggcgaccgg ccggaagccg cgaagcggct agtcgcggtc cgcgatcatc cgctcccgga
     1501 tctccgcggt gaagaccgcg agggtcagcg ggcagggcgg ctcgttcgcg tcgaggtact
     1561 gacagtcctt ctcgctgcac cgcacgaaga gcttgccccg acccaccagc gcgttccgcc
     1621 gccgcagggt gatcgagacc atctcggaga cgacctcgca gcggaacgac ttcgacggca
     1681 tccggctcac gcggtctcct ttgcgacgac gtgagcttcc cgcgagatca tgggaggatg
     1741 gcaggggcgc gatgacctgc cagggtcttc cgcgaagacg ccccgagtat acccgaggcg
     1801 ggctccggcc gccgaggcgt gatttcagcg cgtcggcgtg acgagcgagc ggagcaccgc
     1861 gatgatcgag gagttgtcgc gctcgcccca gccgcccgcg acgccggcga cgaggagctg
     1921 gcgatgcagc gcggtggcga gcagcggagc gccggccgcg tgcccgacct cgagcatcag
     1981 ctcgacgtcc ttgagatgct gggcgagctt gccctcgggc tcgaagcgat cggtgagcat
     2041 gcgctcgccc ttgacgtcca tcgcgcgcga gtaggccgcg ctgtccttca gcaccgcgag
     2101 caggtcggac ccgtcgaggc cctggcggag cccgaacagc aatccttccg cgagtgcgag
     2161 ccggttgagg ccgaggacca ggttgaccac gagcttggcc cgcgcccccg cgccgttgcc
     2221 gcccacgtgg tacgcgcgcc gggcgatggc gtcgaagatc ggccggcagc gctcgaagac
     2281 cgggcggtcg ccgcccacca ccaccggcgc ctcgcgccga cgggcctgcc cgctcgaccc
     2341 cgcgaccggc
//
LOCUS       DATMCV010000098        10013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_406360, whole genome shotgun sequence.
ACCESSION   DATMCV010000098 DATMCV010000000
VERSION     DATMCV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406360"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VNU03_02400"
     CDS             <1..422
                     /locus_tag="VNU03_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HWP73327.1"
                     /translation="MLALVPMVLALLWTIGLMQVFGIKFNLANIWGLPLIIGTAAEFG
                     LNVIVSHLEVRSHPGGPLIARSAVMGVMLNGITTMVGFGSLMIASHQGIFSLGLLLTL
                     GSFCGLVAALVVLPVLLQLMRKGTVAAEVAVEKSSAA"
     gene            complement(563..2257)
                     /locus_tag="VNU03_02405"
     CDS             complement(563..2257)
                     /locus_tag="VNU03_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73328.1"
                     /translation="MPATPPAPSSQRVQFAPVEGAAALFTPEFQAYLLRLHDELGARV
                     YALRAKRDERLVQALQHGRLPSVPASSAAGGDWKVPPVPKDLATPGIEISGPCSITSM
                     FINALNPGPEGERAEGDLDDDEDSAGHRLIDTVRACHNRLAAVNHELHFEDRERKREY
                     RIAPGELPFFMHRERGLHLDEPDVTVDGKPVSAAILGTALTLFYPGRAQAGRGQGIYF
                     YLPKVDGAEECTWYRDLFDRSRAQLPFLEDATIRAIPLVEALPAVFEMEEMLHALGPY
                     AAGLNAARWDLKASIFEFVMADPKAVWPDRFGVDIKTTPFLSNIFRRLVAVCLKRGAV
                     PIGGMATALPNPDPEVNRVAAESIRADKVWEAEQGFIRAWVAHIFHMKAAAEPFKRVH
                     GGGSWKPKPEMVKPESYPVRIEVPAGPITVEGTRRNARMLIEYVEGWLQGRGAKGIDS
                     LAGKPGVHPALMEDLATGRMSVAQIAQRIRHKAKTTQGPEAVHDVALVKRLLQEEVED
                     ILRLLKARDAETETRYRKAVKIALQWIRNYTQLDYRSLGSYTRADLEKIAAAPDAL"
     gene            complement(2271..3026)
                     /locus_tag="VNU03_02410"
     CDS             complement(2271..3026)
                     /locus_tag="VNU03_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWP73329.1"
                     /translation="MSREASAFTDEIDAAVDRLDAARLEREYRAQDEFLVVPRFLPPA
                     AVGALLDDVRRLEPGVNRNYIPRHKKGGSVSHFAIAEEGPALLALYRSDRFRRFLGCL
                     VRADLHLCPDEDPHACALYFYTEAGDHMGYHYDTSYYKGARYTVLLGLEQESSSRLLC
                     RLHTRQPGRPPVEVAVATDPGTMVVFNGDKVYHGVSPSREGERRVVFTMEYVTSREIG
                     RAQRLFSDLKDAFAYFGLRALWRSRARGARVTA"
     gene            complement(3032..5086)
                     /locus_tag="VNU03_02415"
     CDS             complement(3032..5086)
                     /locus_tag="VNU03_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP73330.1"
                     /translation="MRAIVPSVCPHDCPSQCALQVTVDSGRITDVVGDPTHPFTRGVI
                     CGKVHDYAERVYGNTRVSTPLRRVGPKGEGRFEPIGWDEALDEIAHRWRRIIAQWGPE
                     ALLPFSYGGTLGIVQVSAGHPLFHALGASRLERTICISTAYAGWRATLGAVTGSDSEQ
                     MVDSDLIVLWGINASHTHINAMTLVKRARRRGAFVVCIDPYRTDTARLADWHLMPRPG
                     TDGALALGIMHVLVGEGRVDEAYIQRATLGFSALAQHVKQYDPERVARISGVAGDDIV
                     ALARRYGATRAAFIRVGIGLSRHDNGGMTCRTIACLPALTGAYAHPAGGALLSSGGAF
                     GLRAAAVERPDLMPDPAPRSINMIRLGRALTDPDLRPPVKALYVYNANPATIVPNQEL
                     VLQGLRREDLFTVVHEQHLTDTTDYADLVLPATTSMEHTDCYKSYGHLYLQLARPVIP
                     PEGLARSNWTVTRDLARAMGLRDPHFAKSEDDLIREVLGSGDPSVAGITLERLADEHS
                     VRLQVGRPYLPFASGAPTPSGKVEFLSETMARDGLPALPTYVPLVEGPEHAALLERYP
                     LQCIVPPNRFFLNSSFSQSERSRRRQRGPAVLLHPADAAARAIGGGDAVLVRSARGQA
                     RFTAELTEDTRPGVVVVEGIWWSKHQPGGRGVNALTDDRTTDMGGGPALHSNLVQVER
                     FA"
     gene            5163..6422
                     /locus_tag="VNU03_02420"
     CDS             5163..6422
                     /locus_tag="VNU03_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73331.1"
                     /translation="MRDGEAQPGPFSSPEARRLGLLFAIVYFAQGMVYLPDQVVSIVF
                     KERGLTAGQLATFSWIITIPWFIKPVYGLLSDFVPIAGTRRRSYFLIMAALATVAAIA
                     VSLMTGAPYWALAVIITLLWLGVGFTDVLTDALMVENGKPLGLTGTFQSVQWAALSAS
                     SILVGVAGGYLAEHRAFAAAFALVACFPLISLLMAWLVVQEPPARPNLEGLGETWAAL
                     RQATGRRDLWLVAGFIFFWAFSPSFGPAFFYYQTDTLHFSQTLIGALASLGAAASIAG
                     AWVYARLARRIPLKRLIVWSIGAGTVGSLAYIAYGGVVSAAVITVIFGAVGMTTQLAF
                     LDLAAKACPARAEATFFALLMSIYNLGTRSSEWTGANLYDWLGYTPLVLISTVFTAAA
                     WLLVPLVPIDAIQAAAAAPQTEAAPPA"
     gene            complement(6434..6847)
                     /locus_tag="VNU03_02425"
     CDS             complement(6434..6847)
                     /locus_tag="VNU03_02425"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HWP73332.1"
                     /translation="MKPRPQPFRGIGSARKTRRTLERGGIGPAAELNQTLGNWPRVKI
                     TPMFGRWGYFVGPRLFACFPLRAKDTDLWIRLGPEDQRRALAAGCTPHRRMSAGGWVE
                     CRVEGIRDVGRAVRWLRRSYEWARGAVEREERGDS"
     gene            complement(6870..7643)
                     /locus_tag="VNU03_02430"
     CDS             complement(6870..7643)
                     /locus_tag="VNU03_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP73333.1"
                     /translation="MRLKGKIALVTGGAGGIGRATVLALAREGADVAIGYSTSEAEAH
                     ETVRLAEPSGVRALPIKADVTRDDAARAMVDRVADELGGLDCLVNNAGWTRRVPHRRL
                     DDLTDEIWERVLATNLRGPFYCARAAVPHMERRGGGAIVNITSVAGATGTGSSMAYAA
                     SKGGLDTLTKSLARALAPSRIRVNAVAPGLLGTGFGGGLITAELLEQTGRLTPIGRPV
                     TPEEVADAVVFLLANPALTGYIVTVDGGLTALGAADPRW"
     gene            complement(7693..8454)
                     /gene="mce"
                     /locus_tag="VNU03_02435"
     CDS             complement(7693..8454)
                     /gene="mce"
                     /locus_tag="VNU03_02435"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWP73334.1"
                     /translation="MVTNIHHVGIAVQSLKQAYRFWRDTLGLPLAREAEVAEQGVRAA
                     LLSAGETEIELLEPLGPESPIGRFLAKRGEGLHHVCFKTEDVAGDLTALRGKSVALLD
                     AAPRRGLAGRVAFLHPRACHGVLVELATPEPGDPGADSPVRFKRLVIGSPVPHDTAKT
                     YQDLFGLPEVEVNGGPRTMLGWAGGGTLLMVPATEVGGMEGMVALSMVAPDLPPLIQR
                     LERADAALLLGAGELTVEPKSSHGVHLHISRYHFP"
     gene            complement(8524..9621)
                     /locus_tag="VNU03_02440"
     CDS             complement(8524..9621)
                     /locus_tag="VNU03_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP73335.1"
                     /translation="MRGFATQEGTAGYRQRLGTRAARGHFKTWQGLRLSSVGIGTYLG
                     AEDDATDRAYEATVKRALQLGINVVDTAVNYRHQRSERSVGAALRALVAEGEIAREDV
                     VVATKGGFIPFDGAVPANPNAYFTDTYVRPGVLKVADMVGGCHCITPRYLGDQLDRSR
                     ANLGVETIDVYYVHNPEMQLGAVDRPTFMARMRAAFEFLESAVGDGRIRWYGTATWEG
                     YRRPATAPDYLSLPDLVGLAREVAGTRHHFGVIQLPYSLAMTEALAQPNQPIGKASVS
                     VLQAAEQLGVYVMASASIFQGKLARNLPPALSQVLEGLRTDAQRALQFVRSTPGVGTA
                     LCGMTQVAHVEENAALVGLAPAPVAKLFTRG"
     gene            complement(9624..>10013)
                     /locus_tag="VNU03_02445"
     CDS             complement(9624..>10013)
                     /locus_tag="VNU03_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopD"
                     /protein_id="HWP73336.1"
                     /translation="LGPAGFWESMVLPDVLQHRLVVLLVVSLGLFEWMVRSGRLTRPG
                     GRLVFPLLCAAGGGLLLTHSHAMFNLKTEFLTEVSHAPMGLLGVIMAWGRWLEVRLPR
                     ADAWLPGWIWAGAMTIIGLILLVYRET"
BASE COUNT         1341 a         3610 c         3570 g         1492 t
ORIGIN      
        1 gcatgctggc cctggtcccg atggtgctgg cgctgctgtg gaccatcgga ctgatgcagg
       61 tgttcgggat caagttcaac ctcgccaaca tctggggcct gcccctcatc atcggaaccg
      121 cggccgagtt cgggctcaac gtcatcgtga gccatctcga ggtccgctcc cacccgggcg
      181 ggccgctgat cgcccgcagc gccgtgatgg gcgtgatgct gaacggcatc accaccatgg
      241 tgggcttcgg cagcctgatg atcgcctccc accagggcat cttcagcctc gggctgctgc
      301 taaccttggg ctcgttctgc gggctcgtcg ccgcgctcgt cgtgctgccg gtcctgctgc
      361 agctgatgcg caaggggacg gtggcggcgg aggtcgcagt ggagaagtcc tccgcggcgt
      421 agcgtctcgg cggggggact tgcccttgcc ttcgtcaccc gttgccttca ccgccccgtt
      481 gccttcacca ccccgttgcc ttcaccaccc ctcaccctgc cctctcccct gaggggagag
      541 ggggataggg tggggacaac ctttataggg cgtcgggggc ggccgcgatc ttctcgaggt
      601 cggcgcgggt gtaggagccg aggctgcggt agtcgagctg ggtgtagttg cggatccact
      661 gcagcgcgat cttcaccgcc ttgcgatagc gggtttcggt ctcggcgtcg cgcgccttca
      721 gcagccgcag gatgtcctcg acctcttcct ggaggagccg cttcacgagg gccacgtcgt
      781 ggaccgcctc cgggccctgg gtggtcttcg ccttgtggcg aatgcgctgg gcgatctggg
      841 ccaccgacat gcggccggtg gcgaggtcct ccatcagggc cgggtgcacg ccgggcttgc
      901 cggccaggct gtcgatgccc ttggccccgc ggccctgcag ccatccttcc acgtactcga
      961 tcagcatgcg cgcgttgcgc cgggtgccct ccaccgtgat cggccccgcc ggcacctcga
     1021 tgcgcaccgg atagctctcc ggcttcacca tctcgggctt cggcttccag gacccgccgc
     1081 cgtgcacgcg cttgaagggc tcggcggccg ccttcatgtg gaagatgtgg gccacccagg
     1141 cgcggatgaa gccttgctcc gcctcccaga ccttgtccgc ccggatggac tccgcggcca
     1201 cccggttgac ctccggatcc gggttgggca gcgcggtggc catgcccccg atcgggaccg
     1261 cgccgcgctt gaggcacacc gccaccagcc gccggaagat gttggagagg aacggagtgg
     1321 tcttgatgtc caccccgaag cggtccggcc acaccgcctt cggatcggcc atcacgaact
     1381 cgaagatgct cgccttgaga tcccagcgcg ccgcattcag cccggccgcg tagggcccga
     1441 gggcgtgcag catctcctcc atctcgaaca ccgcgggcag cgcctccacg agcgggatgg
     1501 cgcggatcgt cgcgtcctcg aggaacggca gctgcgcgcg actccgatcg aagaggtcgc
     1561 gataccacgt gcactcctcg gcgccgtcca ccttgggcag gtagaagtag atgccctgcc
     1621 cgcggccggc ctgggcgcgc cccggataga agagggtgag cgcggtgccg aggatcgcgg
     1681 cggagaccgg cttgccgtcc accgtgacgt ccggctcgtc gaggtgcagg ccccgctcgc
     1741 ggtgcatgaa gaacggcagc tcgccggggg cgatgcggta ctcccgcttg cgctcgcgat
     1801 cctcgaagtg cagctcgtgg ttcaccgcgg ccaggcggtt gtggcacgcg cgcaccgtgt
     1861 cgatcagccg gtgcccggcc gagtcctcgt cgtcgtcgag gtcgccctcg gcccgctcgc
     1921 cctccggccc ggggttgagc gcgttgatga acatgctggt gatcgagcac ggccccgaga
     1981 tctcgatgcc gggcgtggcc aggtccttcg gcaccggggg caccttccag tcgccgcccg
     2041 ccgcgctcga ggccggcacc gagggtagcc gcccgtgctg gagggcctgg accagccgct
     2101 cgtcgcgctt ggcccgcagc gcgtaaaccc gcgcgccgag ctcgtcgtgc aaccggagca
     2161 ggtaggcctg gaactcgggg gtgaagaggg cggcggcgcc ctccacgggc gcgaactgca
     2221 cccgctggct cgagggggcg gggggagtgg ccggcattga ggtggctcct tcaggcggtt
     2281 acgcgggcgc cgcgcgcgcg gctccgccag agcgccctga ggccgaagta ggcgaacgca
     2341 tccttcaggt cggagaagag acgctgggcg cgcccgatct cccggctcgt cacgtactcc
     2401 atcgtgaaca ccacgcgtcg ctcgccctcg cgggacgggc tgaccccgtg gtagaccttg
     2461 tccccgttga agaccaccat cgtccccggg tcggtggcga ccgccacctc gaccggcggg
     2521 cgcccgggct ggcgggtgtg cagccggcag agcaggcggc tcgacgactc ctgctcgagc
     2581 ccgagcagca ccgtgtagcg cgcgcccttg tagtacgagg tgtcgtagtg gtaccccatg
     2641 tgatcgcccg cctcggtgta gaagtagagc gcgcaggcgt gcgggtcctc gtctgggcag
     2701 aggtggagat cggcgcgcac gaggcagccg aggaagcggc gaaagcgatc ggagcggtag
     2761 agcgcgagca gggccggccc ctcctccgcg atcgcaaagt ggctcacgct cccgcccttc
     2821 ttgtggcgcg ggatgtagtt gcggttgact ccgggctcga gacgccgcac gtcgtcgagg
     2881 agcgcgccca ccgcggcggg gggcaggaac cgcgggacga cgaggaactc gtcctgggcc
     2941 cggtactcac gctcgagccg ggccgcgtcg aggcgatcga ccgccgcgtc gatctcgtcg
     3001 gtgaaggcgg aggcctcgcg gctcatgggg gtcaggcgaa ccgctcgacc tgcaccaggt
     3061 tggagtgcag cgcgggcccg ccgcccatgt cggtggtgcg gtcgtcggtg agcgcgttga
     3121 ccccgcgtcc gcccggctgg tgcttgctcc accagatgcc ttcgaccacc accacccccg
     3181 gccgcgtgtc ctcggtcagc tcggcggtga accgcgcctg cccgcgcgcg ctgcgcacca
     3241 ggaccgcgtc cccgccgccg atggcgcgcg ccgccgcgtc ggccgggtgc agcaggaccg
     3301 ccgggccccg ctggcgccgc cggctccgct cggactggct gaacgatgag ttgaggaaga
     3361 agcggttggg cggcacgatg cactggagcg gatagcgctc gagcagggcc gcgtgctccg
     3421 gcccttccac cagcggcacg taggtgggca gggcgggcag cccgtcgcgg gccatcgtct
     3481 cggacaggaa ctcgaccttg ccggacgggg tgggcgcgcc gctcgcgaac ggcaggtacg
     3541 gtcgacccac ctgcagacgc accgagtgct cgtccgccag gcgctcgagc gtgatgccgg
     3601 ccaccgaggg gtcacccgag cccagcacct cgcggatcag gtcgtcctcg ctcttggcga
     3661 agtggggatc gcgcagcccc atcgcgcgcg cgagatcccg ggtcaccgtc cagttcgagc
     3721 gggccaggcc ctccggggga atcaccgggc gcgcgagctg cagatagaga tgcccgtagg
     3781 acttgtagca gtcggtgtgc tccatcgagg tcgtcgcggg cagcacgagg tcggcgtagt
     3841 cggtggtgtc ggtcaggtgc tgctcgtgca cgaccgtgaa gaggtcctcg cggcggaggc
     3901 cctgcagcac cagctcctgg ttcggcacga tggtggccgg gttggcgttg tagacgtaga
     3961 gcgccttgac cggcggccgc aggtccgggt cggtgagcgc gcgcccgagc cggatcatgt
     4021 tgatcgagcg cggggccgga tcgggcatca ggtcgggccg ctccaccgcc gccgcccgga
     4081 gcccgaaggc gccgccggag gagagcaggg cgccccccgc cgggtgcgcg taggcgccgg
     4141 tcagcgcggg caagcaggcg atggtccggc acgtcatgcc gccgttgtcg tggcgggaga
     4201 gtccgatgcc cacccggatg aacgcggcgc gcgtcgcccc gtagcggcgg gcgagcgcga
     4261 cgatgtcgtc cccggccacc ccgctgatgc gggccacgcg ctccgggtcg tactgcttga
     4321 cgtgctgcgc gagcgcggag aagccgaggg tggcgcgctg gatgtaggcc tcgtccaccc
     4381 ggccctcgcc gacgagcacg tgcatgatcc cgagggcgag ggccccgtcc gttcccgggc
     4441 gcggcatcag gtgccagtcg gcgaggcgcg cggtgtcggt gcggtaggga tcgatgcaga
     4501 ccacgaaggc accgcgccgc cgcgctcgct tcacgagcgt catcgcgttg atgtgggtgt
     4561 gggaggcgtt gatgccccag agcacgatga ggtcggagtc gaccatctgc tcggaatcgc
     4621 tcccggtcac cgcgccgagc gtcgcccgcc agcccgcgta ggcggtgctg atgcagatcg
     4681 tacgctcgag ccgcgaggcc ccgagggcat ggaagagcgg gtggcccgcc gagacctgca
     4741 cgatgccgag cgtcccgccg taggagaacg gcagcagcgc ctccgggccc cactgggcga
     4801 tgatccggcg ccagcgatgc gcgatctcgt cgagcgcctc gtcccagccg atcggctcga
     4861 agcgcccctc gcccttgggc ccgactcggc ggaggggcgt ggagactctg gtattcccgt
     4921 agacccgctc cgcgtagtcg tgcaccttgc cgcagatcac gccccgggtg aacgggtggg
     4981 tgggatcgcc gaccacgtcg gtgatgcggc ccgagtcgac cgtcacctgg agcgcgcact
     5041 gcgacgggca gtcgtgcgga cagacggacg ggacgatcgc tcgcatgagt gcctagatta
     5101 gcataccggc tcgcggcccc cgatcgggcc ccgcccgccg cggagccgcg ctatgatcgg
     5161 ccatgcgcga tggcgaggca cagcccgggc ccttctcctc acccgaggcc cggcgcctcg
     5221 gcctgctctt cgcgatcgtc tatttcgcgc agggcatggt gtacctgccc gaccaggtcg
     5281 tgtccatcgt gttcaaggag cgggggctca ccgccgggca gctcgccacc ttctcgtgga
     5341 tcatcacgat cccgtggttc atcaagccgg tctacggact gctctcggac ttcgtgccga
     5401 tcgcgggcac ccggcggcgc agctacttcc tgatcatggc cgcgctcgcc accgtcgccg
     5461 cgatcgcggt gagcctcatg accggcgcgc cctactgggc gctcgccgtc atcatcaccc
     5521 tgctctggct cggggtgggc ttcaccgacg tgctcaccga cgcgctcatg gtcgagaacg
     5581 gcaagccgct cgggctcacc ggcacgttcc agtcggtgca gtgggccgcg ctgagcgcct
     5641 cgtcgatcct ggtcggggtc gcgggcggct acctcgctga gcatcgcgcc ttcgcggccg
     5701 ccttcgcgct ggtggcgtgc tttccgctga tctcgctgct gatggcctgg ctggtggtac
     5761 aggagccgcc cgcccgcccc aacctcgagg gcctcggcga gacgtgggcc gcgctccggc
     5821 aggcgaccgg ccgccgcgat ctctggctgg tggccggctt catcttcttc tgggccttca
     5881 gcccgtcctt cggccccgcc ttcttctact atcagaccga cactctccac ttctcgcaga
     5941 ccctgatcgg cgcgctggcc tcgctcggcg ccgccgccag catcgcgggg gcgtgggtct
     6001 acgcgcgcct ggcgcggcgc attccgctca agcggctcat cgtgtggtcg atcggggccg
     6061 ggaccgtcgg ttccctcgcc tacatcgcct acgggggcgt ggtctccgcg gcggtcatca
     6121 cggtgatctt cggcgcggtc ggcatgacca cccagctcgc cttcctcgac ctggccgcga
     6181 aggcctgccc cgcgcgcgcg gaggcgacct tcttcgcgct cctgatgtcg atctacaacc
     6241 tgggcacgcg cagctcggag tggaccgggg ccaacctcta cgattggctc ggctacacgc
     6301 ccctggtgct gatctcgacc gtcttcaccg cggccgcctg gctgctcgtg ccgctggtgc
     6361 ccatcgacgc catccaggcc gccgcggccg ccccccagac cgaggccgcg ccgcccgcct
     6421 gaggccggcc gggtcagctg tcgccgcgct cctcccgctc gaccgctccc cgggcccatt
     6481 cataggagcg gcgcagccag cgcaccgcgc gacccacgtc gcggatgccc tcgacgcggc
     6541 actccaccca gccgcccgcg ctcatgcgcc ggtgcggcgt gcagcccgcc gcgagggccc
     6601 gccgttgatc ctcggggccg aggcggatcc agagatcggt gtccttcgcg cggagcggga
     6661 agcaggcgaa gaggcgcggg ccgacgaagt agccccagcg tccgaacatc ggggtgatct
     6721 tgacgcgggg ccagttgccg agcgtctggt tcagctcggc ggcgggaccg atgccgccgc
     6781 gctcgagcgt gcggcgcgtc ttacgcgcgg agccgatgcc gcgaaacggc tggggacgcg
     6841 gcttcacgcc gaccgggccc gccgcgcgcc taccaccgcg gatccgccgc gcccagcgcg
     6901 gtgagcccgc cgtccacggt gacgatgtag ccggtcaggg ccggattcgc gagcaggaac
     6961 accaccgcgt cggcgacctc ctccggggtg accggccgcc cgatcggggt gaggcgtccg
     7021 gtctgctcga ggagctccgc cgtgatgagg ccgcccccga agccggtgcc gagcaggccc
     7081 ggcgccaccg cgttgacccg gatgcgcgac ggggcgagcg cccgggccag cgacttcgtg
     7141 agcgtgtcga gaccaccctt cgaggccgcg taggccatcg aggagccggt gccggtcgca
     7201 cctgccaccg aggtgatgtt gacgatcgcc cccccgcccc gccgctccat gtggggtacc
     7261 gcggcacgcg cgcagtagaa cggcccgcgc aggttggtgg cgaggacgcg ctcccagatc
     7321 tcgtcggtca ggtcgtcgag ccgccggtgc ggtacccgcc gggtccaccc cgcgttgttg
     7381 accaggcagt cgagcccgcc cagctcatcc gcgacgcgat cgaccatcgc gcgcgccgcg
     7441 tcgtcccgcg tgacgtccgc cttgatcggg agcgcgcgca cacccgacgg ctcggccagg
     7501 cgcacggtct cgtgggcctc cgcctccgac gtggagtagc cgatcgcgac gtcggcgcct
     7561 tcgcgggcca gggccagcac ggtcgcgcgc ccgatcccgc cggcgccgcc ggtgaccagc
     7621 gcgatctttc ccttgagtct catgagccct cgagcctcac cggcacgcgg tggagcgtcc
     7681 ggcgggcgct gctcagggaa agtgataccg cgagatgtgc aggtggaccc cgtgagagga
     7741 tttcggctcg acggtcagct ctcccgcgcc gagcaggagc gcggcgtccg ctcgctcgag
     7801 ccgctggatc agcggcggca ggtcgggggc gaccatggag agcgccacca tcccctccat
     7861 gccgccgacc tcggtggccg gcaccatcag gagcgtgccg cctccggccc agcccagcat
     7921 ggtgcgcggc ccaccgttca cctcgacttc cgggagcccg aagaggtcct ggtaggtctt
     7981 cgcggtgtcg tgcggcaccg gcgagccgat cacgaggcgc ttgaagcgca ccggcgagtc
     8041 cgcgcccggg tcgcccggct cgggggtggc cagctcgacg agcacgccgt ggcaggcgcg
     8101 cgggtgcagg aacgcgacgc gcccggccag cccgcggcgc ggggcggcgt cgaggagcgc
     8161 cacgctcttg ccacggagcg cggtgagatc cccggccacg tcctccgtct tgaagcagac
     8221 gtgatggagt ccctcgcccc gctttgcgag gaagcggccg atcgggctct ccgggccgag
     8281 cggctccagc agctcgatct cggtctcgcc cgcggacagg agcgcggccc gcacgccctg
     8341 ctcggccacc tccgcctcgc gcgcgagcgg caagcccagg gtgtcccgcc agaaccggta
     8401 cgcctgcttg aggctctgta ccgcgatccc gacgtgatgg atgttcgtca ccatggctcg
     8461 atcacctcga tcacgctagc cgacgagccc cggattttca aggtctttcg gctcccggcg
     8521 gcctcagcca cgcgtgaaca gcttcgccac cggcgcgggg gccagtccca ccagggccgc
     8581 gttctcctcg acgtgcgcca cctgcgtcat cccgcagagc gcggtcccca cgccgggcgt
     8641 ggatcgcacg aactggagcg cgcgctgggc gtcggtccgc aggccttcca gcacctggct
     8701 gagcgcgggc gggagattcc gggccagctt gccctggaag atcgaggcgg aggccatcac
     8761 gtagacgccg agctgctccg cggcctgcag caccgagacc gacgccttgc cgatcggctg
     8821 gttgggctgc gcgagcgctt cggtcatggc gaggctgtag ggcagctgga tcacgccgaa
     8881 gtggtgccgg gtgcccgcga cctcgcgcgc gagcccgacc aggtcgggca gcgacaggta
     8941 gtcaggcgcc gtggcgggcc ggcgatagcc ctcccaggtc gcggtgccgt accagcggat
     9001 gcgcccgtcg cccaccgcgc tctccaggaa ctcgaaggcc gcgcgcatgc gggccatgaa
     9061 cgtggggcgg tccaccgccc cgagctgcat ctccgggttg tggacgtagt agacgtcgat
     9121 ggtctccacg cccaggttgg cgcggctccg gtcgagctga tctcccaggt agcggggcgt
     9181 gatgcagtgg cagcctccca ccatgtcggc caccttgagc acgccgggcc gcacgtaggt
     9241 gtcggtgaag taggcgttcg ggttggctgg caccgccccg tcgaacggga tgaagccgcc
     9301 cttggtggcc accaccacgt cctcgcgcgc gatctcgcct tcggccacga gcgcgcgcag
     9361 cgccgccccc acgctcctct cgctgcgctg gtggcggtag ttcaccgcgg tgtccaccac
     9421 gttgatcccg agctggagcg cgcgcttcac cgtggcctcg taggcccggt cggtcgcgtc
     9481 gtcctcggcg ccgagatagg tgccgatgcc caccgaggag agccgcagcc cctgccaggt
     9541 cttgaagtgt ccccgggcgg cccgggtccc gagccgctgc cggtagccgg cggtcccctc
     9601 ttgcgtcgcg aagcctcgca tgctcacgtc tcccggtaca cgagcaggat gagtccgatg
     9661 atcgtcatgg ccccggccca gatccagccg ggcagccagg catccgcgcg gggcagccgc
     9721 acctcgagcc agcggcccca ggccatgatc accccgagga gtcccatcgg ggcgtgcgag
     9781 acctcggtga ggaactcggt cttcaggttg aacatggcgt gcgagtgggt cagcagcagg
     9841 ccgccgcccg ccgcgcacag gagcggaaag accaggcgcc cgcccggccg ggtgagccgg
     9901 cccgagcgga ccatccactc gaagagcccg agggacacca cgagcagcac gaccagacga
     9961 tgctggagca cgtcgggcag gaccatgctc tcccagaagc cggcggggcc cag
//
LOCUS       DATMCV010000099         2596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_72687, whole genome shotgun sequence.
ACCESSION   DATMCV010000099 DATMCV010000000
VERSION     DATMCV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72687"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2217
                     /locus_tag="VNU03_02450"
     CDS             <1..2217
                     /locus_tag="VNU03_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP73337.1"
                     /translation="AFVRSPHAHARIRGIKAGAALKVPGVVAVVTGQDLKPHVSPIPV
                     AQASAEGGEVSEVKVGRQHYPLSVDRVRYVGEPVAAVIGLSPEAAMDGAGAVEVDWEP
                     LPAVADPFAAMADGAPQLFDDAPKNIEHSTTIKAGDPDAAFAKAHKIVKQRMNSQRLS
                     GIPMETRAVMAAPDFASGGLTVWATHQAPHVLRGGLADALRMPQNQIRVIAPEVGGGF
                     GVKFGTYPEDVTVAALARLRRVPLRWIESRVEHMTGTTHGRAQMTDLEAAVGADGRIA
                     GLRMHVVADIGAYPIFTFIPDLTLMMGVGVYHVRDVELKSTCVFTNTTSVAAYRGAGR
                     PEAAYYLERLVDCIAMELGKPPEEIRRVNFIPPSAFPYAAPTGQNYDSGEYARALAKS
                     LEVSKYTALRAEQRQRIEKNDRTLLGIGMACYVEMCGFGPYESAMIRVEPSGTVTAFT
                     GASAHGQGHETTFAQLIADYLGVDYDKVVVRHGDTSSAPMGFGTGGSRSLVVGGSAIV
                     KASEKVQERARRIAASMLEAAFEDIVVTDGKYHVKGAPAKSLALAQIAEKAYGEGLPP
                     GMEPGLEATEYFRPPQLVYPFGAHVAVVEVDRDTGRIRLRDYVSVDDCGTRVSPTLVD
                     GQVHGGIAQGVAQSMLEEVVYDRDGQLLTGSLMDYAIPRAEDLPFFVTDQTVTTSPFN
                     PLGAKGIGEAATIGSTPALVNAVVDALKPFGVRHLDMPLRPERVWRAMQNGDHAAR"
     gene            2254..2499
                     /locus_tag="VNU03_02455"
     CDS             2254..2499
                     /locus_tag="VNU03_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73338.1"
                     /translation="MTEADDLRHAIREGIMQGALPREHCRMTWYGPGTGGACVACEQP
                     ITADDVEVECDLQNGGTLRFHRRCYDVWSSEWPTCAP"
BASE COUNT          413 a          891 c          953 g          339 t
ORIGIN      
        1 gcgttcgtgc gaagcccgca cgcccatgcg cggattcggg ggatcaaggc cggcgccgcg
       61 ctcaaggtgc cgggggtggt cgcggtggtc accggccagg acctcaagcc gcacgtcagc
      121 ccgatcccgg tcgcgcaggc ctcggcggaa ggcggtgagg tgagcgaggt caaggtcggc
      181 cgccagcact acccgctctc ggtggaccgg gtgcgctacg tgggcgagcc ggtggccgcg
      241 gtcatcgggt tgtcgccgga ggccgcgatg gacggcgccg gcgcggtcga ggtggactgg
      301 gagccgctgc ccgcggtggc cgaccccttc gccgcgatgg cggacggagc gccccagctc
      361 ttcgacgacg cgccgaagaa catcgagcac tccaccacca tcaaggccgg ggatccggac
      421 gccgccttcg cgaaggccca caagatcgtc aagcagcgca tgaacagcca gcgcctctcc
      481 ggcatcccga tggagacgcg cgcggtgatg gccgcgcccg acttcgcgag cggcgggctc
      541 acggtgtggg ccacgcatca ggcgccccac gtgctgcgcg gcggactggc cgacgcgctc
      601 cggatgcccc agaaccagat ccgggtgatc gcccccgagg tcggcggcgg cttcggggtg
      661 aagttcggga cctatcccga ggacgtgacg gtggcggcgc tcgcgcgcct gcggcgtgtg
      721 ccgttgcgct ggatcgagag ccgggtggag cacatgaccg gcaccaccca cggccgcgcc
      781 cagatgaccg acctggaggc cgcggtggga gccgacggcc gcatcgccgg gctgcgcatg
      841 cacgtggtgg ccgacatcgg cgcctatccg atcttcacgt tcatccccga cctcaccctg
      901 atgatggggg tgggcgtgta ccacgtgagg gacgtggagc tcaagagcac gtgcgtgttc
      961 accaacacca cctcggtggc ggcgtaccgc ggagcggggc gcccggaggc ggcctactac
     1021 ctcgagcggc tcgtggactg catcgcgatg gagctcggca agccgccgga ggagatccgg
     1081 cgggtcaact tcatcccgcc gagcgcgttc ccgtacgcgg cgcccaccgg ccagaactac
     1141 gacagcggcg agtacgcgcg cgcgctcgcc aagagcctcg aggtctcgaa gtacaccgcg
     1201 ctccgcgccg agcagcggca gcgcatcgag aagaacgacc ggacgctgct cggcatcggc
     1261 atggcctgct acgtggagat gtgcggcttc ggcccctacg agagcgcgat gatccgggtg
     1321 gagccgagcg gcaccgtgac cgccttcacc ggggcctccg cgcacgggca ggggcacgag
     1381 accaccttcg cccagctcat cgccgattac ctgggcgtcg actacgacaa ggtggtggtg
     1441 cgccacggcg acacctcgag cgcgcccatg ggcttcggca ccggcggcag ccgcagcctg
     1501 gtggtgggcg gctccgccat cgtgaaggcc tcggagaagg tgcaggagcg cgcccgccgc
     1561 atcgccgcgt cgatgctgga ggccgccttc gaggacatcg tggtgaccga cggcaagtat
     1621 cacgtcaagg gcgcgccggc caagagcctg gccctggccc agatcgcaga gaaggcctac
     1681 ggcgagggcc tgcctcccgg gatggagccg gggctcgagg ccaccgagta cttccgcccc
     1741 ccgcagctcg tctacccgtt cggggcccac gtggcggtgg tcgaggtgga ccgggacacc
     1801 gggcggatcc gcctgcgcga ctacgtctcg gtggacgact gcggcacgcg cgtcagcccg
     1861 acgctggtgg acgggcaggt gcacggcggc atcgcccagg gagtggccca gtcgatgctc
     1921 gaggaagtgg tctacgaccg ggacggccag ctcctcaccg gctcgctgat ggactacgcg
     1981 atcccgcgcg ccgaggacct gccgttcttc gtgaccgacc agaccgtgac cacctccccg
     2041 ttcaacccgc tcggagccaa ggggatcggg gaggcggcca ccatcggctc gactcccgcg
     2101 ctggtcaacg cggtggtgga cgcgctgaaa cccttcgggg tgcggcacct cgacatgccg
     2161 ctccgtccgg agcgggtgtg gcgggcgatg cagaacggcg accacgccgc gcgatagggc
     2221 gccggcgatc gaatcgaacg cgggagtgac ggcgtgaccg aagcggacga tctgcggcac
     2281 gcgatccggg aaggcatcat gcagggcgcc ctcccccggg agcactgccg gatgacctgg
     2341 tacgggcccg gaaccggcgg agcgtgcgta gcgtgtgagc agccgatcac ggccgacgac
     2401 gtggaagtcg agtgcgatct gcagaacgga ggcaccctac gattccaccg ccgctgctac
     2461 gacgtgtggt cgagcgagtg gccgacgtgc gccccctagc gaggaatgac cgtgtaggtt
     2521 gtacgccgac gcgacgaaat ttccccatcg aggtcgcggc cgggaacggt aactacccgc
     2581 gctcgctgct cgggcc
//
LOCUS       DATMCV010000100         5019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_871908, whole genome shotgun sequence.
ACCESSION   DATMCV010000100 DATMCV010000000
VERSION     DATMCV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871908"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1007)
                     /locus_tag="VNU03_02460"
     CDS             complement(66..1007)
                     /locus_tag="VNU03_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP73339.1"
                     /translation="MRVGIMIEGQEGLTWERWQRLVHAAEDMGYESLCRSDHLTGLWG
                     ESKRSSLETWTSLTMAAARSHRIRFGPMVSPVTFYHPALLAKMAVALDNLSGGRMDLG
                     LGGGWNEHEHAMFGIPMPPMKERLDRLECAARYIRALGVGEPVTLEQPYYPLHKAENF
                     PLPTYGRLRLVIGGRGEKRTLRIAAEFADEWNVTRVDPDTFRQKREVLAKHCAAVRRD
                     PESITRSLMIPLAIGRDRHDVTQRIAAARATFPQMPVDEEAWRGASFLVGTPDEVVDQ
                     LREWEEAGVQRVLLQMLDQEDLAAIELFARHVMPRVR"
     gene            1114..2367
                     /locus_tag="VNU03_02465"
     CDS             1114..2367
                     /locus_tag="VNU03_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWP73340.1"
                     /translation="MAPSQRLLLLIPTTTYRTEDFVDAARSLDVDLVIASDRPNVMAT
                     EFPDHLLTLPFADPAAAAREMRRYAARRPLDAVVPVDDATTVVASAVGEALGLRVNPL
                     AAAQATRDKQVMRELLASAGVPSPSFIPVRVDEDPAAAARRAVYPCVLKPRVLSASRG
                     VIRADDEAAFVAAFARIRAILREPDVAALGEGIDTILVEGFVPGIEVALEGLLEDGAL
                     RTLALFDKPDPLDGPFFEETIYVTPSRLPPPTQAAVADVAARAARALGLTDGAVHCEL
                     RLRAGAGGALEPVVLEIAARSIGGLCGRTLRFGTGMSLEELILRRALGRPVDSLERER
                     TAAAVMMIPIPRAGILEEVRGLDAARAVPRIEDITISLHKGQEVVPLPEGSRYLGFIF
                     SRAAAPAEAEAALRAAHRHLQFVIR"
     gene            2406..2828
                     /locus_tag="VNU03_02470"
     CDS             2406..2828
                     /locus_tag="VNU03_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWP73341.1"
                     /translation="MSIVREFERAFNRQDVAALVACFTPDGSYHDTFFGGHAGPDALR
                     AMFERMFREGRDYRWTMETVVETDAGAAAEWTFGYTVTDAVPRSAGRAVRFRGMSFFE
                     KREGRIAAYRESFDLGAALLQLGFSPEAMAKTIRRHRL"
     gene            2830..3792
                     /locus_tag="VNU03_02475"
     CDS             2830..3792
                     /locus_tag="VNU03_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73342.1"
                     /translation="MSTYILRRLVLFVPTLVGASILIFVLMRLVPGDIAEILVYQTGS
                     ESSAVQKKQIQQIRRELGLDRPVVTQYVDWIAGAVRGDFGQSYVQRRPVMDILRERFP
                     RSMELALLTLVLAVVWAIPLGVISAVRQNGPLDYLARLVSLSGLSLPLFFTGALILYG
                     LVRIFHWLPPLEFVSFTEDPLENLKQLIWPALAQAYYISAPITRLTRSQMLEVVRQDY
                     VRTARAKGLAERAVIYRHALRNSLLPVVTFIGWWGGRLLGGLVIMEIIFVVPGMGSAL
                     IQAVSQRDYPTVQAMVFVMALVFLGLNLAIDLLYAWLDPRIRYA"
     gene            3800..4639
                     /locus_tag="VNU03_02480"
     CDS             3800..4639
                     /locus_tag="VNU03_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73343.1"
                     /translation="MTGTVRRFVRGQPLGAAGAVVVLVLIGVALLAPLLAPHGPKETG
                     FATYVAPGREFPMGTDQVGRDVLSRVIWGARLSLYVGLVSVVFGITLGSLWGVVTGYV
                     GGATDTGSQRIVDCLMALPPIILALSLMAALGQSVNNVILSLVILLTPTAVRTVRAVA
                     LGVAATPFIEAAEAAGASRARIVFRHLIPNCMASYIVLVTTNISYAIVVEASLSFIGA
                     GAPPDEPSWGGMLAQGIQALETAPWMVFFPGLAISLAVFGLNLLGDSIRDLTDPRLRG
                     GLG"
     gene            4673..>5019
                     /locus_tag="VNU03_02485"
     CDS             4673..>5019
                     /locus_tag="VNU03_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73344.1"
                     /translation="MSLSRLTRLAALAAVLLATIGPPAGPAGAQEKPRTGGILTWFDY
                     GDPGRLDVHAESPLVVQQATAGIYSGLLQYDPDEPSKIIADLAERWTVSADGKTYTFH
                     LRKNVKWHDGQPF"
BASE COUNT          636 a         1821 c         1775 g          787 t
ORIGIN      
        1 cacgatctcc atggcggaga gcgtggcgag cgcgaccagg ccgtcggtgg tgaggagctc
       61 gctcactacc tcacgcgggg catgacgtgc cgggcgaaca gctcgatcgc ggcgagatcc
      121 tcctggtcga gcatctgtag cagcacgcgc tggacgcccg cctcctccca ctcgcggagc
      181 tgatcgacca cctcgtccgg cgtgcccacc aggaagctcg ccccgcgcca ggcctcctcg
      241 tcgaccggca tctgggggaa cgtggcgcgg gccgccgcga tgcgctgggt cacgtcgtgg
      301 cggtcgcggc cgatcgcgag cgggatcatc agggagcggg tgatcgactc cgggtcgcgg
      361 cgcaccgccg cgcagtgctt cgcgagcacc tcgcgcttct ggcggaaggt gtccgggtcc
      421 acccgggtga cgttccactc gtcggcgaac tcggccgcga tgcgcagggt gcgcttctcc
      481 ccccggccgc cgatcacgag gcgcagccgg ccgtaggtgg gcagggggaa gttctcggcc
      541 ttgtggagcg gatagtaggg ctgctcgagg gtcaccggct cgccgacccc cagggcccgg
      601 atgtagcgcg cggcgcactc caggcgatcg aggcgctcct tcatgggcgg catcgggatt
      661 ccgaacatgg cgtgctcgtg ctcgttccac ccgccgccca gccccaggtc catccgtccg
      721 cccgagaggt tgtcgagggc caccgccatc ttggcgagca gggcggggtg gtagaacgtc
      781 accggcgaga ccatggggcc gaaacggatg cggtgcgatc gggccgcggc catggtgagc
      841 gaggtccacg tctcgagcga ggatcgcttg gattcgcccc agagcccggt gagatgatcc
      901 gaccggcaga gcgactcgta gcccatgtcc tcggccgcgt gcacgagtcg ctgccagcgc
      961 tcccaggtga gcccttcctg cccctcgatc atgatgccga cgcgcatgac ctggcctcct
     1021 gtggtctccg ggatcccggt cgggagccgg gctctcatgt ataccgcaac tgccggccgc
     1081 gtgccccgtg ataaactctg cttcgctgcg gccatggcgc cttcccagcg actcctgctc
     1141 ctgattccca ccaccacgta ccggaccgag gacttcgtcg acgcggcgcg gtcgctcgac
     1201 gtggacctgg tgatcgcctc cgatcgcccg aacgtgatgg cgaccgagtt tcccgatcac
     1261 ctgctcacgc tgcccttcgc ggatccggcc gcggcggccc gcgagatgcg ccggtacgcg
     1321 gcgcgccggc cgctcgacgc ggtggtgccg gtcgacgacg ccaccacggt ggtggcctcc
     1381 gcggtggggg aagccctcgg cctgcgcgtg aacccgctcg ccgccgcgca ggccacccgc
     1441 gacaagcagg tcatgcgcga gctgctcgcg tcggcggggg tgccctcgcc gtcgttcatc
     1501 ccggtgcggg tcgacgagga tcccgccgcg gcggcccggc gcgcggtcta tccctgcgtg
     1561 ctcaagccgc gggtgctctc ggccagccgg ggggtgatcc gggccgacga cgaggcggcg
     1621 ttcgtggccg ccttcgcgcg catccgggcc atcctgcgcg agcccgacgt ggccgcgctg
     1681 ggcgagggga tcgacaccat cctggtggag ggcttcgtgc cgggcatcga ggtcgcgctc
     1741 gagggactgc tcgaggacgg cgccctccgc acgctggccc tcttcgacaa gccggatccg
     1801 ctcgacgggc cgttcttcga ggagacgatc tacgtgaccc cgtcgcggct gcccccgccc
     1861 acccaggccg cggtggccga cgtggccgcg cgggcggccc gcgccctcgg cctcaccgac
     1921 ggcgcggtgc actgcgagct gcgcctgcgc gcgggggcgg ggggcgcgct cgagccggtg
     1981 gtgctggaga tcgcggcgcg ctccatcggc ggcctctgcg ggcggaccct ccgcttcggc
     2041 accggcatgt cgctcgagga gctgatcctg cggcgcgcgc tggggcggcc ggtggactcg
     2101 ctcgagcgcg agcggacggc cgcggcggtg atgatgatcc cgatcccgcg cgccgggatc
     2161 ctcgaggagg tgcgcgggct cgacgcggcc cgcgcggtgc cccggatcga ggacatcacg
     2221 atctcgctgc acaaggggca ggaggtcgtg cccctgccgg agggctcgcg ctacctcggc
     2281 ttcatcttct cgcgcgcggc cgcccccgcg gaggcggagg cggccctgcg cgcggcccat
     2341 cgccacctgc agttcgtgat ccgctgagcc cgcggcgcgc cgccgcgccg cgagggagac
     2401 cggccatgag catcgtgcgg gagttcgagc gcgccttcaa ccggcaggac gtggcggctc
     2461 tggtcgcctg cttcaccccc gacgggtcct accacgacac gttcttcggg gggcacgcgg
     2521 ggccggacgc cctgcgcgcc atgttcgagc gcatgttccg cgagggacgc gactaccggt
     2581 ggacgatgga gacggtggtg gagaccgacg ccggcgcggc cgccgagtgg accttcggct
     2641 acacggtgac cgacgcggtc ccccgcagcg cgggccgcgc ggtgcgcttc cgcggcatga
     2701 gcttcttcga gaagcgtgag ggtcggatcg cggcctaccg cgagtccttc gacctgggcg
     2761 ccgcgctgct gcagctgggc ttcagcccgg aggcgatggc caagaccatc cggcgccatc
     2821 ggctctgagg tgtcgaccta catcctgcgg cggctcgtcc ttttcgtgcc cacgctggtc
     2881 ggcgcctcga tcctgatctt cgtgctcatg cgcctggtgc cgggagacat cgcggagatc
     2941 ctggtctacc agaccggctc cgagtcgagc gcggtgcaga agaagcagat ccagcagatc
     3001 cgccgcgagc tgggcctcga ccgcccggtc gtcacccagt acgtggactg gatcgcgggc
     3061 gcggtgcggg gcgacttcgg ccagtcctac gtgcagcgcc gcccggtgat ggacatcctc
     3121 cgggagcgct tcccgcgctc gatggagctg gccctgctca ccctggtcct cgcggtggtg
     3181 tgggcgatcc cgctcggggt gatctcggcg gtgcggcaga acggccccct cgactacctc
     3241 gcgcggctgg tcagcctgag cggcctcagc ctgcccctct tcttcaccgg ggccctcatc
     3301 ctctatggcc tggtgcggat cttccactgg ctgcctccgc tcgagttcgt gtccttcacc
     3361 gaggatccac tcgagaacct caagcagctc atctggcccg cgctggccca ggcctactac
     3421 atcagcgccc cgatcacccg gctcacccgc tcgcagatgc tcgaggtggt gcgccaggac
     3481 tacgtgcgca ccgcgcgggc caagggcctg gccgagcgcg cggtgatcta ccgccacgcc
     3541 ctgcgcaact cgctcctgcc ggtggtcacc ttcatcgggt ggtggggcgg tcggctcctg
     3601 ggcgggctcg tgatcatgga gatcatcttc gtggtgccgg gcatggggag cgcgctcatc
     3661 caggcggtga gccagcgcga ctatccgacg gtccaggcga tggtcttcgt gatggcgctg
     3721 gtgttcctcg gcctgaacct ggcgatcgac ctgctctacg cgtggctcga tccgcgcatc
     3781 cgctacgcgt gaggccgcga tgaccggcac ggtccggcgc ttcgtccgcg ggcagccgct
     3841 cggcgcggcg ggggcggtgg tggtgctggt cctgatcggg gtggccctgc tcgcgccgct
     3901 gctcgccccc cacggcccga aggagaccgg cttcgcgacc tacgtcgccc cgggccgcga
     3961 gttcccgatg ggcaccgacc aggtcggccg cgacgtgctg agccgggtca tctggggtgc
     4021 gcgcctctcc ctctacgtcg gcctcgtctc ggtggtcttc ggcatcaccc tgggctcgct
     4081 ctggggtgtg gtcaccggct acgtgggcgg ggcgaccgac accggcagcc agcgaatcgt
     4141 cgattgcctc atggccctgc cccccatcat cctcgcgctg tcgctgatgg ccgcgctggg
     4201 ccagtcggtc aacaacgtga tcctgtccct ggtcatcctg ctcaccccca ccgcggtgcg
     4261 gacggtgcgc gcggtggcgc tcggggtggc ggccaccccg ttcatcgagg ccgcggaagc
     4321 ggcgggcgcc tcgcgggcgc gcatcgtgtt ccggcacctg atcccgaact gcatggcgag
     4381 ctacatcgtg ctcgtcacca ccaacatctc gtacgcgatc gtggtcgagg cctcgctgag
     4441 cttcatcggc gcgggcgccc cgcccgacga gccctcgtgg ggcggcatgc tcgcgcaggg
     4501 catccaggcg ctcgaaaccg caccgtggat ggtattcttc cccggactgg ccatcagcct
     4561 ggccgtgttc ggcctcaacc tactcggcga ttcgatccgc gacctcacgg atccgcgcct
     4621 ccgcggcggc ctcggctagc cgcggactcg caaaggagcg atagcgatgc cgatgagcct
     4681 ctcgagactc actcgcctcg cggccctggc cgccgtgctg ctcgccacga tcgggccgcc
     4741 ggccggaccg gcgggcgccc aggagaagcc ccgcaccggc ggcatcctga cctggttcga
     4801 ctacggcgat cccgggcgtc tggacgtcca cgcggagtcg ccgctggtgg tccagcaggc
     4861 gaccgccggc atctacagcg gcctcctgca gtacgacccg gacgagccca gcaagatcat
     4921 cgcggatctc gcggagcgct ggaccgtctc ggccgacggc aagacctaca cctttcacct
     4981 gcgaaagaac gtgaagtggc acgacggcca gccgttcag
//
LOCUS       DATMCV010000101         2840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_538223, whole genome shotgun sequence.
ACCESSION   DATMCV010000101 DATMCV010000000
VERSION     DATMCV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538223"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1063
                     /locus_tag="VNU03_02490"
     CDS             206..1063
                     /locus_tag="VNU03_02490"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWP73345.1"
                     /translation="MPPTSPGIEPLVTALQAQVEALHAREPEVRASRDPEDLRRMRVA
                     VRRLRATLRASRPLFGRSAVDGLRDELDWLGTSLGRVRDLDMVNAAVAAELGSLEGST
                     RRESHALLRRLDADRTRAWDRLHAVLDGARYARLLRRLKTLLGRDPRHAAKVSLADAA
                     GAEWKKLRRAVKHLPGHPSAAELHEIRIRLKRSRYAAELARGAGGRRAEKFIEQAKAV
                     QDILGEHQDAVVIEEYLHDVIDRREAAHALEQQLVRRLRQRRKKTRAAFFHEWPRLAR
                     RGRKAWATA"
     gene            1082..1579
                     /locus_tag="VNU03_02495"
     CDS             1082..1579
                     /locus_tag="VNU03_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWP73346.1"
                     /translation="MNVLLIRHAPAVRTGTGGVRDHDRPLTRRGRQRFRLAARGLARI
                     AGRPDILLTSPLVRARDTAEIAAAAFKRVTPRIEPALGHDDVDVLVTALKKHRPDATV
                     ALVGHEPTLSGLLAHLLGMRDGNGRFAFKKGGAALVHLPDGPSMPGQLLWFVKPRILR
                     SVGSP"
     gene            1582..2556
                     /locus_tag="VNU03_02500"
     CDS             1582..2556
                     /locus_tag="VNU03_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73347.1"
                     /translation="MARPKGTRGDGTPTSDLVAIVDLGSAAVRFLLAQIRPTSGFRVL
                     AQERVPTRLGGGAPGTLPRGAIARTLRAVHRFFARHSPQSQGPRIVAIATSAVRDARN
                     RERLLDPLRRNEGIEVQVLSARDEARFGVVAALESLSFAKGVVVDLGGASLQLSRVRR
                     RRIVSTASLPLGAVRTTRQFLRRDPPTPLELRALRVEIRARLQEALPPADRGEVLVGL
                     GGTVRTLASIHLRANPGERKHRHGLVLLQSDVTAIRERLEALSSHGRRRIRGLKAERA
                     DIILAGAMVIEDVMISGGYLHLVICTRGVRDGVLLHEASRSRAALSPR"
     gene            complement(2542..>2840)
                     /locus_tag="VNU03_02505"
     CDS             complement(2542..>2840)
                     /locus_tag="VNU03_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWP73348.1"
                     /translation="KQADPEVVEEWKWRGVPVWSHDGLICTGETYKNVVKMTFSRGAA
                     LEDPSGLFNASLEGNTRRAIDFHEGEKIDEKALKALIRAAVTLNASRGHGLSRR"
BASE COUNT          385 a         1073 c          982 g          400 t
ORIGIN      
        1 ccatcccggt cttcagaaag acccgtcgct tcacgaatac ccccccttga tcgagtggat
       61 cgagtgcgcc gcgtggtggc aatggtacag ggccacgtag aaacgtccaa ttcgttgtcc
      121 cgatgaggcc gacaggcccc ataggtgact cctatgtcga cagcggctgg cggcggcggc
      181 gcgcccatgg taccgattgg agcccatgcc gcccacgagt cccggcatcg agccccttgt
      241 cacggcgctc caggcccagg tcgaggccct ccacgcccgc gagcccgagg tccgggcgag
      301 ccgcgatccg gaggatctcc ggcggatgcg ggtcgcagtc cgccggctgc gggccaccct
      361 ccgggcgagc cgtccgctgt tcggccggag cgcggtcgat gggctgcggg acgagctcga
      421 ttggctcggt acgtcgctcg gtcgggtgcg cgacctcgat atggtgaacg ccgccgtcgc
      481 ggcggagctg ggctcgctcg agggctcgac ccgccgggag agccacgcgc tcctccgtcg
      541 tctcgacgcc gaccgcaccc gggcgtggga ccggctgcac gccgtgctgg acggcgcacg
      601 atacgcccgg ctcctccggc gcctcaagac gctcctcggg cgtgacccgc gccacgccgc
      661 caaggtctcg ctggcggatg ccgccggggc cgagtggaag aagctgcggc gggcggtgaa
      721 gcacctcccg ggccatccga gcgcggccga gcttcacgag atccgcatcc gcctcaagcg
      781 ctcacgctac gcggccgagc tggcgcgagg cgcgggaggc cggcgggccg agaagttcat
      841 cgagcaggcc aaggcggttc aagacattct tggagaacat caggacgccg tggtaatcga
      901 agagtatctg cacgacgtca tcgaccgccg cgaggccgcg catgcgctgg agcagcagct
      961 cgtcagacgg ctacgccagc gccgcaagaa gacgcgcgcc gcgttcttcc acgagtggcc
     1021 gcgcctcgca cgccgcggac gcaaggcctg ggcgacggcc tagccgggcg accctccctc
     1081 gatgaacgtg ctcctgatcc gccacgcgcc cgcggtgcgc accggaaccg gcggggtccg
     1141 cgaccacgac cggccgttga cccgccgggg gcggcagcgc ttccggctgg ccgcccgcgg
     1201 gctggcccgc atcgcgggtc ggcccgacat cctgctgacg agcccgctcg tccgcgcccg
     1261 cgacaccgcc gagatcgcgg ccgccgcgtt caagcgggtc acgccccgga tcgagcccgc
     1321 gctcgggcac gacgacgtcg acgtgctggt caccgcgctg aagaagcacc gccccgacgc
     1381 gaccgtggcg ctggtcgggc acgagccgac cctgtccggg ctgctcgcgc acctgctcgg
     1441 catgcgcgac ggcaacggcc gcttcgcgtt caagaagggc ggcgcggcgc tcgtgcacct
     1501 gccggacggg ccgtccatgc ccgggcagct cctctggttc gtcaagcccc ggattctccg
     1561 gtcggtcggc tcaccctgac cgtggcacga ccgaagggca cgcgagggga cggcaccccg
     1621 acctcggacc tggtggccat cgtcgatctc ggctcggccg cggtccgctt cctgcttgcc
     1681 cagatccgtc ccacgtcggg gttccgcgtc ctcgcccagg agcgcgtgcc gacccgcctc
     1741 gggggcggcg cgccggggac cctgccccgc ggggccatcg ccaggaccct gcgggcggtg
     1801 caccgcttct tcgcccgcca ctcgccccag agccagggcc cgcgcatcgt ggccatcgcc
     1861 acctccgcgg tgcgcgacgc gcgcaatcgg gagcgcctcc tcgatccgct ccggcgcaac
     1921 gagggcatcg aggtgcaggt gctcagcgcc cgggacgagg cgcgcttcgg ggtggtggcc
     1981 gcgctcgaga gcctctcctt cgccaagggc gtcgtcgtcg acctcggcgg ggcgagcctc
     2041 cagctcagcc gggtgcgtcg acgccggatc gtctcgaccg cgagcctgcc gctcggcgcg
     2101 gtgcggacca cccggcagtt cctgcggcgc gatcccccga ccccgctgga gctgcgcgcg
     2161 ctgcgcgtgg agatccgcgc ccggcttcag gaggcgctgc cgcccgccga ccgcggcgag
     2221 gtcctggtgg ggctcggggg caccgtccgc acgctcgcca gcatccacct gcgcgcgaac
     2281 ccgggggagc gcaagcaccg ccacgggctc gtgctgctcc agtcggacgt caccgcgatc
     2341 cgcgagcgcc tcgaggccct ctcttcccac ggtcgccggc ggattcgcgg tctcaaggcc
     2401 gagcgcgccg acatcatcct cgccggggcg atggtcatcg aggacgtgat gatcagcggc
     2461 ggctatctcc acctcgtcat ctgcacccgc ggggtccggg acggcgtcct gctacacgag
     2521 gcctcgagga gcagagccgc cctatctccg agataggccg tggccgcggg acgcgttcag
     2581 cgtcaccgcc gcgcgaatga gagccttcaa tgccttctcg tcgatcttct cgccctcgtg
     2641 gaagtcgatg gcacgcctgg tgttgccctc gaggctcgcg ttgaagaggc ctgacgggtc
     2701 ctcgagcgcg gcccccctgg aaaaggtcat cttcaccacg ttcttgtacg tctcgccggt
     2761 gcagatcaat ccgtcgtgag accacaccgg gacccctctc cacttccact cctcgaccac
     2821 ctcggggtcg gcctgcttga
//
LOCUS       DATMCV010000102         2320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_937896, whole genome shotgun sequence.
ACCESSION   DATMCV010000102 DATMCV010000000
VERSION     DATMCV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937896"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /gene="boxB"
                     /locus_tag="VNU03_02510"
     CDS             <1..1280
                     /gene="boxB"
                     /locus_tag="VNU03_02510"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HWP73349.1"
                     /translation="KEVYLRTATSVDAKGWAQFDYTRMPEYRWGIFLAEPEPGRQINF
                     GDHLGQPAWQEVPGEYRGTLRRLIVTQGDTEPASVEQQRHLGRTCPSLYDLRNLFQVN
                     VEEGRHLWAMVYILDAHFGRDGREESEALLQRRSGDHDKPRILGAFNERTPDWLSFFM
                     FCFFTDRDGKYQLASLAESGFDPLSRTCRFMLTEEAHHMFVGESGVGRIVQRACEIMR
                     EHKTDDVRRHGGVDLPTIQKYINFHCSVSLDLFGSEVSTNAANFYTMGLKGRFEETKK
                     KDDHRLKDGTYTVATLAGDKIVTREEGALVSLNERLRDDYIADCQRGLNRWNEIIKSH
                     GIDFTLTLPHRGFRRAIGAFAEVKVSPDGRVVGEAEWDARHRDWLPTDEDQAYVGSLM
                     QPVTERGKVANWIAPPARGINSQAADFEYVRLG"
     gene            1294..1800
                     /locus_tag="VNU03_02515"
     CDS             1294..1800
                     /locus_tag="VNU03_02515"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP73350.1"
                     /translation="MRAVTSKTLGPTFGMYSHGMIAPGGELVVVAGQVAADRAGKLVG
                     PGDAVAQTRQAFENVRAVLEAAGSSMRQVVRFQTFLTHAADIESFMQARKELFPRYFP
                     DAVYPPNTILVVSRLVLPDLLVEIEAMAVKPAGAAAKAPAPARKPAAKPARPARATGR
                     GKASRRRG"
     gene            1797..>2320
                     /locus_tag="VNU03_02520"
     CDS             1797..>2320
                     /locus_tag="VNU03_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP73351.1"
                     /translation="MMSVPARFNAASFFIDRHVAEGRADKVAFFHDGGSITYGALQEL
                     VNRAGNALLDLGVRPEQRVLCLLLDSPEFLATFWGAIKIGAIPIPVNTMMRGDDYQYF
                     LDDSRAPIAVVSEPLLAEAGPVLPKARYLRHVVVAGRASGTQIRFDDWVAKASPRLEP
                     FDASKDDPAFWLYSS"
BASE COUNT          365 a          813 c          812 g          330 t
ORIGIN      
        1 ccaaggaggt ctacctgcgc accgcgacca gcgtggacgc caagggctgg gcccagttcg
       61 actacacccg gatgccggag taccgctggg gcatcttcct cgccgagccg gagccgggcc
      121 gccagatcaa cttcggcgac cacctgggcc agcccgcgtg gcaggaggtg cccggcgagt
      181 accgcgggac gctcagacgg ctgatcgtga cgcagggaga taccgagccc gcgtcggtcg
      241 agcagcagcg gcacctcggc cggacgtgcc cgtcgctcta cgatctgcgc aacctcttcc
      301 aggtcaacgt ggaggagggc cgccacctgt gggcgatggt gtacattctc gacgcccact
      361 tcgggcgcga cggccgcgag gagtcggagg cgctgctgca gcgccgctcg ggcgaccacg
      421 acaagccccg catcctgggc gccttcaacg agcggacgcc ggactggctc tcgttcttca
      481 tgttctgctt cttcaccgac cgggacggca agtaccagct cgcgagcctg gccgagagcg
      541 gcttcgaccc gctgtcgcgc acctgccgct tcatgctgac cgaggaagcc caccacatgt
      601 tcgtgggcga gagcggggtg gggcgcatcg tgcagcgggc ctgcgagatc atgcgggagc
      661 acaagaccga cgacgtgcgg cgccacgggg gagtggacct gcccaccatc cagaagtaca
      721 tcaacttcca ctgctcggtc tcgctcgatc tgttcggctc cgaggtctcc accaacgcgg
      781 ccaacttcta caccatgggc ctcaagggcc gcttcgagga gaccaagaag aaggacgatc
      841 accggctcaa ggacggcacc tacaccgtgg cgacgctggc cggggacaag atcgtcaccc
      901 gcgaggaggg cgcgctcgtc tcgctcaacg agcggctccg cgacgactac atcgcggact
      961 gccagcgcgg gctgaaccgc tggaacgaga tcatcaagag ccacggcatc gacttcacgc
     1021 tcactctgcc ccatcgcggc ttccggcgcg ccatcggcgc cttcgcggag gtcaaggtgt
     1081 cgccggacgg ccgggtcgtc ggcgaggcgg agtgggacgc gcgccatcgc gactggctcc
     1141 ccaccgacga ggaccaggcc tacgtgggca gcctcatgca gccggtgacc gagcgcggca
     1201 aggtcgccaa ctggatcgcg ccgcccgcgc gcggcatcaa cagccaggcc gccgatttcg
     1261 agtacgttcg tctcggctga ggcgggagac gccgtgagag cggtcacctc gaagacgctg
     1321 ggaccgacgt tcgggatgta ctcgcacggc atgatcgcgc cgggtggcga gctggtggtg
     1381 gtggcgggac aggtggcggc ggatcgggcc gggaagctgg tcggccccgg cgacgcggtg
     1441 gcccagaccc gccaggcgtt cgagaacgtg cgggcggtgc tcgaggcggc ggggtcgagc
     1501 atgcgccagg tcgtgcgctt ccagaccttc ctcactcacg cggccgacat cgagagcttc
     1561 atgcaggcgc ggaaggagct cttcccgcgc tacttcccgg acgcggtcta cccgcccaac
     1621 accatcctgg tggtctcgcg cctcgtgttg cccgacctgc tggtcgagat cgaggcgatg
     1681 gcggtgaagc cggccggggc cgcggccaag gcgccggccc ccgcgcgcaa gccggcggcg
     1741 aagccggcgc gccccgcccg cgccaccggc cgcggcaagg cgtcgcggag gcgcggatga
     1801 tgtcggtgcc ggcgcggttc aacgcggcga gcttcttcat cgaccgccac gtcgccgagg
     1861 ggcgcgcgga caaggtggcc ttcttccacg acggcggctc catcacgtac ggggcgctcc
     1921 aggagctggt caatcgcgcg ggcaacgccc tgctggacct gggggtgcgg ccagagcagc
     1981 gggtgctctg cctgctgctg gactcgcccg agttcctcgc caccttctgg ggcgcgatca
     2041 agatcggagc gatccccatc ccggtcaaca ccatgatgcg gggcgacgac taccagtact
     2101 tcctcgacga cagccgcgca ccgatcgcgg tcgtctcgga gcccctgctg gccgaggcgg
     2161 gcccggtgct gccgaaggcc cgttacctgc gccacgtggt ggtggccggc cgcgcctccg
     2221 gcacgcagat ccgcttcgac gactgggtgg ccaaggcgag cccgcggctc gagcccttcg
     2281 acgcctccaa ggacgatccc gcgttctggc tctactcgtc
//
LOCUS       DATMCV010000103         4465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_337805, whole genome shotgun sequence.
ACCESSION   DATMCV010000103 DATMCV010000000
VERSION     DATMCV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337805"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VNU03_02525"
     CDS             <1..816
                     /locus_tag="VNU03_02525"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73352.1"
                     /translation="LGYVEGRDLKFEIRAANEDFERLPDLAADLVRSGVDLIVAVSSP
                     AIRAAENATSTIPIVMLSGTDPVAGLFVASLAHPEGNVTGLTTYVPELAGKRLELLRE
                     TVPDLRRVAILANLRNPSSAVEVKEMETAARALSIEIHVQDARLPEQYPNAFVSVARG
                     GWRALVVTADPVLSSNRERILQLAARHRLPTMYEWKEIVEIGGLMSYGPSLAEVNGRV
                     AVYVDKILKGASPATLPVERPTRFELAVNLKTAASLGLKFPAALVARADRVVK"
     gene            complement(838..2094)
                     /locus_tag="VNU03_02530"
     CDS             complement(838..2094)
                     /locus_tag="VNU03_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73353.1"
                     /translation="MSLRSTRRTFLGGLTAGAVGVPTLLRAQPRTVKIGMINPVSGPM
                     ADVGQDQRLGAQLAVEAINAAGGVKSLGGARLELLTADSETKADVARTEADRLINAGA
                     QIMTGAYHSAHVATLSLLAQQRRVPYVIDISAADAPTANAARSVREGQQKTQYVYRIF
                     PGSVTFGRNAVRYMTEIFREAGVSPKRIVLMYSNDLFGKTQTDSFQAALKAASPGYEV
                     VDVIPYPENAVDLSTEVARARSAKPDVLAPVTRPATAILLLEELARQRADLMGIISPG
                     APGLYETKQIQQLKDKLEYVMDCTVYANYRKPETQKIAAEYAKRSGGRHFDASGAYAY
                     EAIQVIADVLERAASTDPDAVVAAIRKTSFAGGVTVSTGPVVFNEVGDNPNASTAMLQ
                     ILGQAPRVVWPRDVAEQKFVFPRPRA"
     gene            2328..3170
                     /locus_tag="VNU03_02535"
     CDS             2328..3170
                     /locus_tag="VNU03_02535"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73354.1"
                     /translation="MEAFRQGLREAGVVENRDLVLEVVWTNNELEVSQAVVRLVQRGA
                     KLLIPVGTTASMTVKRQAPTTSILFISVGNPLGIGLVESLSRPGGHVTGFGDFLAELS
                     SKYVQFALEVGKPPGVVHYLWHAGWTDGQYRLQRTEQAAQSLNVKLRARAVTDVSEVD
                     EAMAGLRKAGASVVIVQPSPFTFLQRGRLIESATASGLATIFAFRPAASAGALITYGP
                     DYVDLNRRAAAYLGRILKGTKPGDLPVEQPTKFELLINLKTAKAVGIAMPPSLLVRAD
                     QVIE"
     gene            3177..>4465
                     /locus_tag="VNU03_02540"
     CDS             3177..>4465
                     /locus_tag="VNU03_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP73355.1"
                     /translation="MAVARGRGGHDVIVVGAGHNGLVAACYLARAGRRVLVLERRALV
                     GGACVTEETFPGYKVSTAAYVNSLFRQEIVRDLRLREYGFEVLARNPSSFTPLPDGRS
                     LTLGPDPEWTRREIAKFSPRDAARYPEYEAMLERVAAFVEPTLGMTPPDLLHPGPRDL
                     LRLLGLGRAFGRLGDGASEAVELLTGPARPILDRWFESAELKATLATDAIIGAMASPS
                     TPGTGYVLFHHVMGETDGRRGVWGYVRGGMGGLTQALARAARDLGVEIRCEAEVARIL
                     TREGRVTGVALASGDEHRAPVVASNADAHVTFLRLLAPETLPPAFRDAVGRISYESAS
                     LKINVALAELPDFRACPGTTPGPQHRGTIHVCPDLDYIERAFDDAKYGRPSARPVLEC
                     TIPSVVDPTVVPPGRHLMSIFVQYAPYALRGATWDETR"
BASE COUNT          588 a         1588 c         1628 g          661 t
ORIGIN      
        1 ctgggctacg tggagggccg cgacctcaag ttcgagatcc gggccgccaa cgaggatttc
       61 gagcgcctgc ccgacctggc cgcggacctg gtccgctccg gggtggacct gatcgtggcg
      121 gtgtcctcgc ccgcgatccg cgccgccgag aacgcgacct ccacgatccc gatcgtgatg
      181 ctgagcggca ccgatccggt ggcgggcctg ttcgtggcga gcctggccca cccggagggc
      241 aacgtgaccg ggctcaccac ctacgtgccg gagctggccg gcaagcgcct cgagctgctc
      301 cgcgagaccg tgccggacct gcgccgggtg gccatcctgg ccaacctccg caacccgagc
      361 agcgcggtcg aggtgaagga gatggagacg gcggcgcggg cgctctcgat cgagatccac
      421 gtgcaggacg cccgcctgcc cgagcagtac ccgaacgcct tcgtgagcgt cgcgcggggc
      481 gggtggcggg ccctggtggt caccgcggac ccggtgctgt cgagcaaccg ggagcggatc
      541 ctccagctcg cggcgcgcca tcgcctgccc accatgtacg agtggaagga gatcgtcgag
      601 atcggcgggc tcatgtccta cggcccgagc ctggccgagg tcaacggccg ggtcgcggtc
      661 tacgtggaca agatcctgaa gggggccagc ccggccacgc tgccggtgga gcggcccacc
      721 cgcttcgagc tcgcggtcaa cctcaagacc gcggccagcc tgggtctgaa gttccccgcc
      781 gcgctggtgg cccgcgcgga tcgcgtcgtc aagtagcgcc gcgccggcgc gctcgcgtca
      841 ggcgcggggc cgcgggaaca cgaacttctg ctcggccacg tcccggggcc acaccacgcg
      901 cggcgcctgc ccgaggatct ggagcatcgc ggtggaggcg ttcgggttgt cgcccacctc
      961 gttgaagacc accggcccgg tggagacggt caccccgccc gcaaaggacg tcttgcggat
     1021 cgcggccacc accgcgtcgg gatcggtgga ggcggcgcgc tcgagcacgt ccgcgatgac
     1081 ctggatcgcc tcgtaggcgt aggccccgct cgcgtcgaag tggcgcccgc ccgagcgctt
     1141 ggcgtactcc gccgcgatct tctgggtctc cggcttgcgg tagttcgcgt agaccgtgca
     1201 gtccatcacg tactcgagct tgtccttgag ctgctggatc tgcttcgtct cgtagaggcc
     1261 gggcgcgccc gggctgatga tgcccatgag atcggcgcgc tgccgcgcca gctcctcgag
     1321 caggaggatc gcggtggccg gccgggtgac cggggcgagc acgtccggtt tggccgagcg
     1381 ggcgcgcgcc acctcggtgg agagatcgac cgcgttctcg gggtagggaa tgacgtcgac
     1441 cacctcgtag ccggggctgg ccgccttcag cgcggcctgg aagctgtcgg tctgggtctt
     1501 gccgaagagg tcgttgctgt acatgagcac gatccgcttc gggctcaccc ccgcctcacg
     1561 gaagatctcg gtcatgtagc ggaccgcgtt gcggccgaag gtgacgctgc ccgggaagat
     1621 ccggtagacg tactgggtct tctgctggcc ctcgcgcacc gaccgggccg cgttggcggt
     1681 gggggcgtcc gccgccgaga tgtcgatgac gtagggcaca cgccgctgct gggccaggag
     1741 cgagagcgtg gccacgtggg ccgagtggta ggccccggtc atgatctgcg cgcccgcgtt
     1801 gatcaggcgg tcggcctcgg tgcgcgccac gtccgccttg gtctcgctgt ccgcggtcag
     1861 tagctcgagc cgggcgcccc cgagcgactt gaccccgccc gccgcgttga tcgcctccac
     1921 cgcgagctgg gcccccagcc gctggtcctg ccccacgtcg gccatcgggc cgctcaccgg
     1981 gttgatcatg ccgatcttca ccgtccgcgg ctgggcccgc agcagggtgg ggacgccgac
     2041 ggcccccgcg gtgaggccgc ccaggaatgt gcgtcgcgtc gatcgaaggc tcatgacgca
     2101 ggagacagta gcatacgttc accccggtgg tgtaaggtcg gcccggccgc ccgcctcgtt
     2161 ctcggctcgc ggccaggagg aggcgagatg cgccgacggg cgttcctgtg tagcgtggcg
     2221 ggcggcctcc tcgcggcggc gccggtcgcc gcgcagtcgc cgggcggtca cccctgatcg
     2281 gattcctgcc gctgggcgcg ccgtccaacc cgtacgaccg gtcactcgtg gaggcgtttc
     2341 gacagggcct gcgtgaagcg ggagtcgtcg agaaccgcga cctcgtgctc gaggtggtgt
     2401 ggaccaacaa cgagctggag gtctcgcagg cggtggtccg gctcgtgcag cgcggggcga
     2461 agctgttgat cccggtgggg acgaccgcgt cgatgacggt gaagcggcag gccccgacga
     2521 cgtcgatcct cttcatcagc gtcggcaacc cgctgggcat cgggctcgtc gagagcctct
     2581 cgcggccggg cgggcacgtc accgggttcg gggatttcct ggccgagctc agcagcaagt
     2641 acgtgcagtt cgccctcgag gtggggaagc cgccgggggt ggttcactac ctctggcacg
     2701 cgggctggac ggacgggcag taccggctcc agcgcaccga gcaggcggcc cagtcgctca
     2761 acgtcaagct ccgggcccgc gcggtgaccg acgtctccga ggtcgacgag gcgatggccg
     2821 ggctccggaa ggccggcgcg tcggtggtca tcgtccagcc gagcccgttc acgttcctcc
     2881 agcgcggccg attgatcgag tcggccaccg cgagcggact ggccacgatc ttcgcgttcc
     2941 ggcccgcggc ctcggccggc gccctgatca cctacggccc cgactacgtc gatctcaatc
     3001 gacgggccgc ggcctatctg ggccggatcc tcaagggcac caagcccggc gatctcccgg
     3061 tcgagcagcc gacgaagttc gagctgctga tcaatctcaa gaccgcgaag gcggtcggca
     3121 tcgcgatgcc gccgtcgctg ctggtgcggg cggatcaggt catcgagtag ggtcgcatgg
     3181 cggtcgcgag gggacgcggt ggccacgacg tcatcgtggt gggagccggt cacaacggcc
     3241 tggtcgccgc gtgctacctg gcccgcgcgg gccgccgggt gctggtgctc gagcgccgcg
     3301 ccctggtggg cggcgcgtgc gtgaccgagg agaccttccc gggctacaag gtctccaccg
     3361 cggcgtacgt caacagcctc ttccgccagg agatcgtccg ggacctgcgt ctccgcgagt
     3421 acggcttcga ggtgctcgcg cgcaacccgt cctcgttcac cccgctgccc gacgggcgct
     3481 cgctcaccct gggcccggat cccgagtgga cccgccggga gatcgccaag ttcagcccgc
     3541 gcgacgcggc ccgctatccc gagtacgagg cgatgctgga gcgcgtggcc gccttcgtcg
     3601 agcccaccct cggcatgacc ccgcccgacc tgctgcaccc cggcccgcgc gacctgctcc
     3661 ggctgctggg cctcggccgc gcgttcggcc gcctgggcga cggcgcctcg gaggcggtcg
     3721 agctgctcac cgggcccgcg cgccccatcc tcgaccggtg gttcgagtcg gccgagctca
     3781 aggcgacgct cgccaccgac gcgatcatcg gcgcgatggc gagcccgtcg acgccgggca
     3841 ccggctacgt gctcttccat cacgtgatgg gtgagaccga cggccggcgc ggggtgtggg
     3901 gctacgtgcg cggcgggatg ggcgggctca cccaggccct ggcccgggcc gcgcgcgatc
     3961 tgggggtgga gatccgctgc gaggcggagg tggcgcgcat cctgacgcgg gagggacggg
     4021 tcaccggggt ggcgctcgcg agcggcgacg agcatcgtgc cccggtggtg gcgagcaacg
     4081 ccgacgccca cgtgaccttc ctgcggctgc tcgcccccga gacgctgccg cccgcgttcc
     4141 gcgacgcggt ggggcgcatc tcctacgaga gcgcgtcgct caagatcaac gtggccctcg
     4201 ccgagctgcc cgacttccgg gcgtgcccgg gcaccacgcc gggcccgcag caccgcggga
     4261 cgatccacgt ctgccccgac ctcgactaca tcgagcgcgc cttcgacgac gccaagtacg
     4321 gccggccgtc cgcgcggccg gtgctcgagt gcacgattcc ctcggtggtg gatccgacgg
     4381 tcgtcccgcc cggccgccac ctgatgtcga tcttcgtgca gtacgcgccc tacgccctgc
     4441 gcggcgcgac ctgggacgag acgcg
//
LOCUS       DATMCV010000104         5047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_204760, whole genome shotgun sequence.
ACCESSION   DATMCV010000104 DATMCV010000000
VERSION     DATMCV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204760"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VNU03_02545"
     CDS             <1..618
                     /locus_tag="VNU03_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP73356.1"
                     /translation="IETDQVLMAVGRAARTRGLGLEPLSVATEKGFVTASSSMETSVK
                     GLYAIGDMAGHQMLAHKAMAEGVVAAETIAGQTPRPVDYGNVPSCTYCRPQVASIGVS
                     EATARAGGREIAVGKFPFTASGKAVALGDTEGFVKVVADKSTGEILGVHIVGPEATEL
                     IHEFAVGRTLEATLEEIVHTIHAHPTLSEAALEATLAALGHPIHI"
     gene            659..1816
                     /locus_tag="VNU03_02550"
     CDS             659..1816
                     /locus_tag="VNU03_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73357.1"
                     /translation="MDFRLTPEQEALRLRARELADGTFRARAAGWDATEQYPWDNVKD
                     LVRAGFMGLTIPREYGGAGAPILDVLLVVEEIARVCGVTARIVVEGSLGVVGALAAYG
                     SEAQKRRYFPWVLDGDKPAIAITEPEAGSAATDLVTRADEAPEGYALTGEKRWITGAG
                     TSRLYLVYCRMSRAPGAAGIGGILVERDAPGFRIGDRDHTMGLRGIPEGRLHFDSCRV
                     PKDQVLVGPPEGFKQLMQAYNSQRLGAATVALGLAQGAYELALAYAGQRRQFGRAIGE
                     FQGIRWKLADMAIRLDAARLLIHRAAVNAGDGFPDALEAAKAKTFAAEIAQEVTGQAL
                     QIHGAAGYGRGLPLERMARDARMFAIGGGTVEMMRNLIADRLLPTRADFRR"
     gene            complement(1901..2503)
                     /locus_tag="VNU03_02555"
     CDS             complement(1901..2503)
                     /locus_tag="VNU03_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HWP73358.1"
                     /translation="MAEVELPKPEELHEQAENRFSRRVALVTALFAVVLAIAALGGNH
                     AMKEMLLSQQQSSDQWAFYQAKVIREHLYRGQKLRLEADLVERDATLKPEARKKLEGL
                     LAKLEEEEKRYNVEKKDIEKAAKALEHQRDGFAARDPYFLVAEALLQIAIVMASVSIL
                     ARSGLIFSFSLGLAVIGAVLTLNGYFMLFQLPFMHGGGHH"
     gene            complement(2517..2831)
                     /locus_tag="VNU03_02560"
     CDS             complement(2517..2831)
                     /locus_tag="VNU03_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWP73359.1"
                     /translation="MSEERGNDAAGYLGWFFLGAIAGAATAVLLTPKTGRETRELLAE
                     HSGDLFKRAQEAAGQAQDRAGDLFDKGREYFEEQAQRLTSAFEAGRSAMKDEITRGRS
                     SN"
     gene            complement(2860..3237)
                     /locus_tag="VNU03_02565"
     CDS             complement(2860..3237)
                     /locus_tag="VNU03_02565"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HWP73360.1"
                     /translation="MTPLAVAVILACIVALTVVLISMLLSFKRAAQRAEGVLAIVERE
                     IRPMTTELENLTAELQKLTHKANDSLDRIGTVVERVEDISVTAARVVGAVGGLTRIGQ
                     YAGLAAGVKRGFEVFLTRLKDRH"
     gene            3358..4908
                     /locus_tag="VNU03_02570"
     CDS             3358..4908
                     /locus_tag="VNU03_02570"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HWP73361.1"
                     /translation="MSLHDRLDELRRRNEAAELAGGADRIARQHGAGKKTARERIELL
                     LDKGSFAEMDRLVVHQTHDFGMDEQRIPGDGVVTGGGRIHGRPVFVFAQDFTVFGGSL
                     SEAYARKICKIMDLALKTGTPIVGLNDSGGARIQEGVVSLAGYADIFLRNTLASGVVP
                     QISAIMGPCAGGAVYSPAITDFVFMVKHSSYMFVTGPDVIRAVTHEEVSFEELGGAAT
                     HGGTSGVAHFGAENEEECLALIRELLTFLPQNNSEDPPVRASLDPVDRVDEELQTVVP
                     EQPNRPYDIKDIVRPVLDDRYFFEVQADFAPNLVIGFGRLGGRPVGIVANQPAHLAGC
                     LDINASVKGARFVRFCDCFNLPIVTFEDVPGFLPGTAQEFGGIIKHGAKLLFAYCEAT
                     VPKLTVITRKAYGGAYCVMSSKHIRGDANFAFPTAEIAVMGPDGAVNILYRREMEAAS
                     DPAGLKEEKVREYREKFANPYAAAERGYIDEVIEPRDTRRRLIQVLEVLHTKRDSNPP
                     KKHGNIPL"
BASE COUNT          769 a         1783 c         1756 g          739 t
ORIGIN      
        1 atcgagaccg atcaggtgct gatggcggtg gggcgcgccg cgcgcacccg cgggctcggg
       61 ctcgagccgc tctcggtcgc caccgagaag ggcttcgtga cggcgtcgtc cagcatggag
      121 acctcggtga agggcctcta cgccatcggg gacatggcgg gccaccagat gctggcccac
      181 aaggcgatgg ccgagggggt ggtcgcggcc gagacgatcg cgggccagac gccgcgcccg
      241 gtggactacg gcaacgtgcc ctcctgcacc tactgccgtc cgcaggtggc ctccatcggg
      301 gtgtcggagg cgaccgcgcg cgcgggcggg cgggagatcg cggtcgggaa gttcccgttc
      361 accgccagcg gcaaggcggt cgcgctcggc gacaccgaag ggttcgtcaa ggtggtggcc
      421 gacaagagca ccggcgagat ccttggcgtg cacatcgtcg gccccgaggc caccgagctg
      481 atccacgagt tcgcggtggg ccggaccctc gaggccaccc tggaggagat cgtgcacacc
      541 atccacgccc accccacgct ctccgaggcc gcgctcgagg ccacgctcgc cgccctcggc
      601 caccccatcc acatatgaac cctctcccct ctggggagag ggcagggtga ggggcggcat
      661 ggactttcgc ctcaccccgg agcaagaagc gctgcgcctc cgcgcccgcg agctggccga
      721 cgggaccttc cgcgcccgcg ccgcgggctg ggacgcgacc gagcagtacc cctgggacaa
      781 cgtcaaggac ctggtgcggg ccggcttcat gggcctcacc atcccgcggg agtacggagg
      841 cgccggcgcc ccgatcctgg acgtcctcct ggtcgtcgag gagatcgcgc gggtgtgcgg
      901 ggtcaccgcg cgcatcgtgg tcgaggggag cctgggggtg gtgggcgcgc tcgcggccta
      961 cgggagtgaa gcgcagaagc gccgctactt cccctgggtg ctcgacggcg acaagccggc
     1021 catcgcgatc accgagccgg aggcgggatc cgcggccacc gatctcgtga cccgcgccga
     1081 cgaggcgccg gagggctacg cgctcaccgg cgagaagcgc tggatcaccg gcgccgggac
     1141 ctcgcggctc tacctcgtct actgccggat gagccgcgcg ccgggtgccg cgggcatcgg
     1201 cggcatcctc gtcgagcgcg acgctccggg cttccgcatc ggtgatcgag accacaccat
     1261 gggcctccgg ggcatcccgg aggggcggtt gcacttcgac agctgccgcg tgccgaagga
     1321 ccaggtgctg gtgggcccgc ccgaagggtt caagcagctg atgcaggcct acaacagcca
     1381 gcggctgggt gcggccaccg tcgcgctcgg gctggcccag ggcgcctacg agctggcgct
     1441 ggcctacgcg gggcagcggc ggcagttcgg gcgggccatc ggggagttcc agggcattcg
     1501 gtggaagctc gccgacatgg cgatccggct cgacgcggcc cggctcctca tccatcgcgc
     1561 cgccgtgaat gcgggcgacg gattcccgga tgcgctcgag gcggccaagg ccaagacgtt
     1621 cgcggccgag atcgcgcagg aggtgaccgg ccaggcgctg cagatccacg gcgccgccgg
     1681 gtacggccgg gggctgccac tcgagcggat ggcgcgcgac gcgcgcatgt tcgcgatcgg
     1741 gggcgggacg gtggagatga tgcggaacct catcgccgat cggctgctgc ctacgcgggc
     1801 tgactttcgg cggtagggcg cagcaccccg cccctgaccc tcgcacccct caccttcgcg
     1861 cccctcaccc tgccctctcc ccagagggga gagggagagg tcagtggtgg ccgccgccgt
     1921 gcatgaaggg gagctgaaag agcatgaagt agccgttgag ggtgagcacc gcgccgatca
     1981 cggccagccc gaggctgaac gagaagatca gcccggagcg ggccaggatc gagaccgacg
     2041 ccatcacgat ggcgatctgg agcagcgcct ccgcgacgag gaagtacgga tcgcgggccg
     2101 cgaagccgtc ccgctggtgc tcgagcgctt tcgccgcctt ctcgatgtcc ttcttctcga
     2161 cgttgtagcg cttctcctcc tcctcgagct tggcgagcag gccctcgagc ttcttccggg
     2221 cctcgggctt gagggtggcg tcacgctcga cgaggtcggc ctcgagcctg agcttctgcc
     2281 cgcggtagag gtgctcgcgg atgaccttgg cctggtagaa cgcccactgg tcggaggact
     2341 gctgctggga cagcagcatc tccttcatcg cgtggttgcc gccgagggcc gcgatggcca
     2401 ggaccaccgc gaagagggcc gtgaccagcg cgacccggcg gctgaaccga ttctcggcct
     2461 gctcgtgcag ctcctcgggc ttgggaagct cgacctcggc cattggatca gggcgctcag
     2521 ttgctggagc ggccccgcgt gatctcgtcc ttcatggccg agcgccccgc ctcgaaggcg
     2581 gaggtgagcc gctgggcctg ctcctcgaag tactcgcggc ccttgtcgaa caggtcgccg
     2641 gcccgatcct gcgcctggcc ggccgcctcc tgggcgcgct tgaagaggtc gccgctgtgc
     2701 tcggcgagca gctcgcgcgt ctcccgcccg gtcttgggcg tcagcagtac cgccgtcgcc
     2761 gcgcccgcga tggcgcccag gaagaaccat cccagatagc ccgccgcgtc gtttccacgc
     2821 tcttcgctca tggtcttctc cttttcgccg atcggtcgtt cagtgccgat ccttcagccg
     2881 ggtgaggaac acctcgaatc cgcgcttcac ccccgccgcg agcccggcgt actggccgat
     2941 gcgcgtgagc ccgcccaccg cacccaccac gcgcgccgcg gtcaccgaga tgtcctcgac
     3001 gcgctcgacc acggtgccga tgcggtcgag gctgtcgttg gccttgtggg tgagcttctg
     3061 cagctccgcg gtcaggttct ccagctcggt ggtcatcggg cggatctcgc gctccacgat
     3121 ggccaggaca ccctccgccc gctgggcggc gcgcttgaac gacagcagca tggagatgag
     3181 cacgaccgtg agagccacga tgcaggcgag gatcacagcg accgccagcg gggtcatcgg
     3241 ggttcgagta tggcaggggc tcccgagcaa gtcaaacaaa ccgggcgaga agcacccgca
     3301 ttcattgact ttccgctttc cggcccccta tactccagca gcagaggtcc ccctgtcatg
     3361 tccctccacg atcgcctcga cgagctgagg cgccgcaacg aagcggcaga gctcgccgga
     3421 ggcgccgatc gcatcgcccg ccagcacggg gcgggcaaga agaccgcgcg cgagcggatc
     3481 gagctgctgc tcgacaaggg ctcgttcgcc gagatggatc gcctggtcgt ccaccagacc
     3541 cacgacttcg ggatggacga gcagcggatc cccggcgacg gggtggtcac cggcggcggc
     3601 cgcatccacg ggcggccggt gttcgtgttc gcccaggact tcaccgtctt cggcggctcg
     3661 ctgtcggagg cgtacgcgcg gaagatctgc aagatcatgg acctggctct caagacgggc
     3721 accccgatcg tcgggctcaa cgactcgggc ggcgcccgca tccaggaggg cgtggtctcg
     3781 ctggccggct acgcggacat cttcctccgc aacacgctcg cctccggagt cgtcccgcag
     3841 atctccgcga tcatgggtcc ctgcgccggg ggcgcggtct attcgcccgc catcaccgac
     3901 ttcgtgttca tggtcaagca cagctcctac atgttcgtga ccgggccgga cgtgatccgg
     3961 gcggtcaccc acgaggaggt ctcgttcgag gagctgggcg gcgccgccac ccacgggggc
     4021 acgtcggggg tggcccactt cggggcggag aacgaggaag aatgcctcgc cctcatccgc
     4081 gagctgctca cgttcctgcc ccagaacaat tcggaagacc cgcccgttcg cgcgagcctc
     4141 gacccggtcg atcgcgtcga cgaggagctg cagacggtgg tgccggagca gccgaaccgg
     4201 ccctacgaca tcaaggacat cgtgcggccc gtcctcgacg accgctactt cttcgaggtc
     4261 caggccgact tcgcgcccaa cctggtcatc ggcttcggcc ggctcggcgg gcgcccggtg
     4321 gggatcgtgg ccaaccagcc cgcgcacctc gccggctgcc tcgacatcaa cgcctcggtg
     4381 aagggcgccc gcttcgtgcg cttctgtgac tgcttcaatc tcccgatcgt gaccttcgag
     4441 gacgtgcccg gcttcctgcc cggcaccgcc caggagttcg gcggcatcat caagcacggg
     4501 gccaagctgc tcttcgccta ctgcgaggcg acggtgccca agctcaccgt gatcacccgc
     4561 aaggcctacg gcggcgccta ctgcgtcatg tcctccaagc acatccgggg cgacgcgaac
     4621 ttcgcgttcc ccaccgcgga gatcgcggtg atggggccgg acggggcggt gaacatcctc
     4681 taccggcggg agatggaggc ggcgtccgac ccggccgggc tcaaggaaga gaaggtccgc
     4741 gagtaccgcg agaagttcgc gaacccctac gcggccgccg agcgcgggta catcgacgag
     4801 gtcatcgagc cccgggacac gcggcggcgg ctgatccagg tcctcgaggt cctccacacc
     4861 aagcgcgaca gcaacccgcc caagaagcac gggaatatcc cgctatgacc cggcccctat
     4921 gaccccggaa gagcaggcgc gcttcgagca ggcccgccgc gagtgggagg agcgcaccct
     4981 caagccctcg ctggcccgag ccgccgagcg cccgggcccc tttgcggatt cgagcggcgc
     5041 gccgctg
//
LOCUS       DATMCV010000105         6004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_538345, whole genome shotgun sequence.
ACCESSION   DATMCV010000105 DATMCV010000000
VERSION     DATMCV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538345"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VNU03_02575"
     CDS             <1..451
                     /locus_tag="VNU03_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP73362.1"
                     /translation="ELDEAVWRKVLDIKLTGAFLLCKRVVPIMVRQKWGRIVNLSSIA
                     GKRGSPNTAAYNAANFGIQGLTQSLAMEVARHGVTVNAVCPGTIETARMDVLGRGDGW
                     QRQLERIPMGRAATDEEVAGLIAFLCSPAAAFMTGQSVNIDGGVVMW"
     gene            466..1065
                     /locus_tag="VNU03_02580"
     CDS             466..1065
                     /locus_tag="VNU03_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWP73363.1"
                     /translation="MRECCEVRSDVPARQRRVLQLVLALNAVMFVVELSAGIRGHSTS
                     LVADSVDMLGDAIVYGASLYALGRGALWQARVALTKGLIMAAFGIGVLAEVGAKLSRG
                     LMPAADVMGSIGLLALAANAVCLVLLWRHRADDLNMRSAFVCSLNDVAGNAGVLLASA
                     GVGLLGSAWPDIAVGLLIAVMFGVSAVGVILRARRQLAA"
     gene            complement(1089..1616)
                     /locus_tag="VNU03_02585"
     CDS             complement(1089..1616)
                     /locus_tag="VNU03_02585"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWP73364.1"
                     /translation="MTLRDGSRIRLRPIRPEDQDRLIAFSDRLSQHTSYQRFFSAMRR
                     LPTDWAHLLANVDYQRRLALLAELGPPEAPELIGVARYEPTDRPDTAEIAFVIQDGWQ
                     NRGLGRIMLGDLLAAAEARGVRRFRAYVLASNVRMLDLLKRYTDVQERRMESGVINIL
                     LARRAGDASAPGPTR"
     gene            complement(1645..2829)
                     /gene="kbl"
                     /locus_tag="VNU03_02590"
     CDS             complement(1645..2829)
                     /gene="kbl"
                     /locus_tag="VNU03_02590"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HWP73365.1"
                     /translation="MQGEIRQRLQAELDGIREAGLWKGERVVEGPQGAHVPVDGREVL
                     NFCANNYLGLADDPTLVRAAQDGLTRWGLGLASVRFICGTQSIHKDLEAAIAGFLGMD
                     DAVLYTSCFDANGGLFETLLDEPDVVISDALNHASIIDGVRLCRAQRQRYAHGDMDEL
                     ERLLKAASSARTRLIATDGVFSMDGDVAPLGPICDLAERHDALVMVDDSHATGFFGPT
                     GRGTPEHCGVAGRVDIVTSTLGKALGGASGGFTAGRAEVVALLRQRSRPYLFSNTLPP
                     ALVCSALAGLALLSRSTKLRDRLAANTVWFRQAMTAAGFGLRPGEHPIVPIMLGDARL
                     AHRMAADLFDEGIYVVGFSYPVVPRDKARIRVQVSAAHTEGDLERAVSAFVKVGRALG
                     VI"
     gene            complement(2845..3891)
                     /locus_tag="VNU03_02595"
     CDS             complement(2845..3891)
                     /locus_tag="VNU03_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWP73366.1"
                     /translation="MLDSETESVARKPVILVTGAGGEVGHGLILRLAEQGVHDVVGVD
                     ARSIPDEVARRCLVTQIGDVLDRHLLERLRSEFEIPIVFHLAALLSTRAEFVPETAHE
                     VNVQGTLNLLHLAVDEARSLGHAVKFLFPSSIAVYGFPSREAKHAAGRVAERAALEPV
                     TMYGCNKLYCEHLGRYFAKHYRQLAAGEPSGVDFRAIRFPGLLSAFTLPSGGTSDYAP
                     EMIHAAAQGRPNACFVREDTRIPFMAMPDAIAALLALLEAPAASLTGSVYNVAAFNPS
                     AGELADLVGRAFPDARITFVPDPRRQVVIDSWPEDVDDTAARRDWGFHPAYDLKRAFE
                     EYLTPNIRRHYPPR"
     gene            3960..5831
                     /locus_tag="VNU03_02600"
     CDS             3960..5831
                     /locus_tag="VNU03_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWP73367.1"
                     /translation="MTPIRGSLAPFLDPASVAVIGASRDPFKVGGAVVANLRSAGFPG
                     RIVPVNSRASTVQGLPSAPRLQDVEGAVDLAVIAVPAVDVLPALVQCVAKGVGGAVVI
                     SAGFRETGGEGARREAELRAWLRGQPIRVLGPNCLGWIRPARRLNVTFASGMPEPGGI
                     AFLSHSGAVAVAILDWARDRRVGFSLFASLGNQADVNESDLLDAGAANPETRVIAGYI
                     EGVADGPRFLDALRRAAAVKPVVLLKAGRSAEGARAVASHTGALAGSDRAFDAAARQA
                     GALRVETVAELFDVARGLASQPLPRGRRLVVVTNGGGLGIVATDAARAAGFTMEAPAE
                     PLRQRLAEGLPTMAGLANPVDLVGDADARRYGHALHTLGPDGADAMLVLLTPQATTDA
                     ASVARTIAAATQGWSIPVVAAFVGGARVAPGVRTLEEAGIPCYAFPEPAVKTLAGMVR
                     LGERRRTVPGPSGASVDAAAARVWLAASGASPARRLGLPELRALLAGYGIACADGGPV
                     KAPDDATPAGKELLLGGVRDAQWGPVLTVGLGGIYVEVVGDTAVALAPVSPAEATALL
                     DELRIAPLLDRPALAGIISRFSHLLVDLPELAEIEINPLMTSPDGAIAMDARARLGA"
BASE COUNT          752 a         2135 c         2276 g          841 t
ORIGIN      
        1 cgagctggac gaggcggtct ggcgcaaggt gctcgacatc aagctgaccg gcgcgttcct
       61 cctctgcaag cgcgtggtcc cgatcatggt ccggcagaag tgggggcgca tcgtcaacct
      121 ctcctcgatc gcgggcaagc gaggcagccc caacaccgcc gcctataacg cggccaactt
      181 cggcatccag gggctcaccc agtcgctcgc gatggaggtg gcgcgccacg gggtcaccgt
      241 gaacgcggtc tgcccgggca ccatcgagac ggctcgcatg gacgtgctcg gccggggcga
      301 cggctggcag cggcagctcg agcgcatccc gatggggcgc gccgccaccg acgaggaggt
      361 ggccggcctg atcgcgttcc tctgctcccc cgcggccgcc ttcatgaccg gccagtccgt
      421 caacatcgac ggcggcgtcg tcatgtggtg aggagccccg ccgccatgcg ggagtgctgt
      481 gaggtccggt ccgacgtgcc ggcgcgccag cgccgggtgc tccagctcgt gctcgcgctc
      541 aacgcggtga tgttcgtggt cgagctcagc gccgggatcc gcggccactc cacgtccctg
      601 gtggccgact cggtcgacat gctgggggac gcgatcgtct acggagcgag cctctacgcc
      661 ctcgggcgcg gcgccctctg gcaggcgcgg gtcgcgctca ccaagggcct gatcatggcc
      721 gccttcggga tcggggtcct cgccgaggtc ggagcgaagc tcagccgggg gctgatgccc
      781 gcggccgacg tcatgggctc gatcggcctc ctcgcgctcg ccgccaacgc agtctgcctc
      841 gtcctgctgt ggcggcaccg cgccgacgac ctcaacatgc gctccgcctt cgtgtgctcg
      901 ctcaacgacg tggccggcaa cgcgggcgtg ctgctggcga gcgcgggcgt ggggctgctc
      961 gggtcggcct ggcccgacat cgcggtcggg ctgctgatcg cggtgatgtt cggtgtctcc
     1021 gcggtcgggg tgatcctgcg ggcgcggcgg cagctcgcgg cctgagcggg cgggcgccgc
     1081 cgctcgggtc agcgcgtcgg gcccggcgcg ctcgcgtcgc cggcgcggcg cgccagcagg
     1141 atgttgatca cgccgctctc catccgccgc tcctgcacgt cggtgtagcg cttgagcaga
     1201 tcgagcatgc gcacgttgct cgcgagcacg taggcgcgga agcggcgcac gccgcgcgcc
     1261 tccgcggcgg caagcaggtc gcccagcatg atccggccaa gtcctcggtt ctgccagccg
     1321 tcctggatca cgaacgcgat ctcggcggtg tccggccgat cggtcggctc gtagcgggcc
     1381 accccgatga gctccggcgc ctctggaggc ccgagctcgg ccaggagagc gagccgccgc
     1441 tggtagtcca cgttggcgag caggtgggcc cagtcggtcg gcaggcgccg catcgcgctg
     1501 aagaagcgct ggtaggaggt gtgctggctc agccgatctg agaaggcgat caggcggtcc
     1561 tggtcctcgg gccggatggg ccgcagccgg atccgcgatc cgtcccggag ggtgatctcc
     1621 cgctcgagct cggcgggata cgtgctagat caccccgagc gcgcggccga ccttgacgaa
     1681 cgcggacacc gctcgctcca ggtcgccttc cgtgtgggcc gcgctcacct ggacccggat
     1741 gcgcgccttg tcgcgcggca ccaccgggta ggagaagccc accacataga tgccctcgtc
     1801 gaacaggtcg gcggccatcc gatgggcgag ccgcgcgtcc ccgagcatga tcggcacgat
     1861 gggatgctcg cccgggcgca gcccgaagcc cgccgcggtc atcgcctgcc ggaaccacac
     1921 ggtgttggcc gcgaggcggt cccgcagctt ggtcgacctc gagaggagcg cgaggccggc
     1981 gagggccgag cagaccaggg cgggcggcag ggtgttcgag aagagatagg ggcgcgagcg
     2041 ctggcgcagc agggcgacga cctcggcccg gcccgcggtg aagccgccgc tggccccgcc
     2101 cagggccttg ccgagcgtcg aggtgacgat gtccacgcgc ccagcgacgc cgcagtgctc
     2161 cggggtgccc cggccggtcg gcccgaagaa gccggtggcg tgactgtcgt ccaccatcac
     2221 gagcgcgtcg tggcgctcgg cgagatcgca gatcgggccg agcggcgcca cgtcgccgtc
     2281 catcgagaac accccgtcgg tggcgatcag ccgggtgcgc gcggacgagg ccgccttcag
     2341 gaggcgctcg agctcgtcca tgtcgccgtg ggcgtagcgc tggcgctggg cccggcagag
     2401 ccggacgccg tcgatgatcg aggcgtggtt gagcgcgtcc gagatgacga cgtcgggctc
     2461 gtccaggagt gtctcgaaga gcccgccgtt ggcgtcgaag caggaggtgt agagcaccgc
     2521 gtcgtccatg ccgaggaagc ccgcgatcgc cgcctcgagg tccttgtgga tggactgggt
     2581 gccgcagatg aaccgtacgc tggcgagccc caggccccag cgggtgaggc cgtcctgggc
     2641 ggcgcggacg agggtcggat cgtcggcgag cccgaggtag ttgttggcgc agaagttgag
     2701 gacctcgcgc ccgtccaccg ggacgtgcgc gccctgcggg ccttcgacga cgcgctcgcc
     2761 cttccacagc cccgcctcgc ggatgccgtc gagctcggcc tgcagccgct gccggatctc
     2821 accttgcatg gtcgtcctcc cgggtcagcg cggcgggtag tgccgccgga tgttcggcgt
     2881 caggtattcc tcgaaggccc gcttgagatc gtaagccgga tggaagcccc agtcgcgccg
     2941 ggccgcggtg tcgtcgacgt cctcgggcca cgagtcgatg acgacctggc ggcgcgggtc
     3001 cgggacgaac gtgatccgcg cgtccgggaa ggcccgccct acgaggtcgg ccagctcgcc
     3061 cgcgctcggg ttgaaggccg cgacgttgta gaccgagccg gtgagcgacg cggcgggcgc
     3121 ctccagcagg gccaggaggg cggcgatcgc gtcgggcatg gccatgaacg ggatgcgggt
     3181 gtcctcgcgg acgaagcagg cgttgggccg cccctgggcc gccgcgtgga tcatctccgg
     3241 cgcatagtcg ctcgtgcccc cgctcggcag ggtgaacgcg gagagcaggc ccgggaagcg
     3301 gatcgcccgg aagtccaccc cgctcggctc cccggccgcg agctgccggt agtgcttcgc
     3361 gaagtaccgt ccgagatgct cgcagtagag cttgttgcac ccgtacatcg tgaccggctc
     3421 gagcgccgcg cgctccgcca cccggcccgc ggcgtgcttc gcctcccggc tcgggaagcc
     3481 gtagaccgcg atcgagctgg ggaagaggaa cttcaccgcg tggccgagcg agcgcgcctc
     3541 gtcgacggcc aggtggagga ggttgagggt gccctggacg ttcacctcgt gggcggtctc
     3601 cgggacgaac tcggcccggg tcgacagcag cgcggccagg tggaagacga tcgggatctc
     3661 gaactcgctc cggagccgct cgagcaggtg gcgatccagg acgtcgccga tctgggtgac
     3721 caggcagcgt ctggccacct cgtcggggat ggagcgggcg tcgacgccca cgacgtcgtg
     3781 gacgccctgc tccgcgagcc ggaggatcag gccgtgcccg acctcgcctc ccgccccggt
     3841 cacgagtatt accggcttgc gcgccacgct ctcggtctcc gaatcgagca taacagggtc
     3901 aacgctccat gaaagggggc aacaccggga gatggaggcg cgctatcatc tcgtcgacca
     3961 tgactccgat ccgcggttcg ctcgccccgt tcctcgaccc ggccagcgtc gcggtgatcg
     4021 gggcctcgcg cgatccgttc aaggtcggcg gggcggtggt ggccaacctg cgctcggccg
     4081 gcttcccggg ccgcatcgtc ccggtcaact cgcgggcgag tacggtgcag gggcttccgt
     4141 cggcgccgcg cctgcaggac gtggagggcg cggtggacct ggccgtgatc gcggtgcccg
     4201 cggtcgacgt gctccccgcg ctcgtgcagt gcgtggccaa gggggtgggc ggcgcggtgg
     4261 tgatctccgc cggcttccgg gagaccggag gggagggcgc ccgccgggaa gccgagctcc
     4321 gcgcgtggct gcgcgggcag ccgatccggg tgctcggccc gaactgtctc ggctggatcc
     4381 gccccgcgcg acggctcaac gtgacgttcg cctccgggat gcccgagccg ggcgggatcg
     4441 ccttcctgtc gcactccggc gcggtcgcgg tggcgatcct cgactgggcg cgcgaccggc
     4501 gggtcggctt ctcgctcttc gcctcgctcg gcaaccaggc cgacgtcaac gagagcgatc
     4561 tgctcgacgc gggggccgcc aatccggaga cccgggtcat cgccgggtac atcgaggggg
     4621 tggccgacgg tccccggttc ctcgacgcgc tccgtcgcgc cgccgcggtc aagccggtgg
     4681 tgctgctgaa ggcgggccgc tccgccgagg gcgcgcgcgc cgtcgcctcg cacaccggcg
     4741 cgctcgccgg gtccgaccgc gccttcgacg cggcggcccg tcaggcgggc gcgctccgag
     4801 tcgagaccgt cgccgagctg ttcgatgtcg cgcgggggct cgccagccag cccctgccgc
     4861 ggggccgtcg cctggtcgtg gtgaccaacg ggggcgggct cggcatcgtc gccacggatg
     4921 cggcgcgcgc ggccggcttc accatggagg cacccgccga gccgctgcgc caacggctcg
     4981 ccgagggatt gccgacgatg gccgggctcg ccaacccggt ggacctggtg ggcgacgccg
     5041 acgcgcgccg ctacggccac gcgctgcaca cactcggccc cgacggcgcg gacgcgatgc
     5101 tcgtgctcct gaccccgcag gcgaccaccg acgcggcgag cgtggcgcgc accatcgccg
     5161 ccgcgacgca gggatggtcg atcccggtgg tggccgcctt cgtgggcggc gcgcgggtgg
     5221 cgcccggggt gcgcacgctc gaggaggccg ggattccctg ctacgcgttt cccgagcccg
     5281 cggtcaagac gctggccggc atggtgcgcc tcggcgagcg ccggcgcacg gtccccggcc
     5341 cgtccggcgc ctccgtcgac gcggccgccg cccgcgtgtg gctcgccgcg agcggcgcct
     5401 cgccggcgcg ccggctcggc ctgcccgagc tgcgcgcgct gctcgccggg tacggcatcg
     5461 cgtgcgccga cggcggcccg gtcaaggcgc ccgacgacgc gacgccggcc ggcaaggagc
     5521 tgctgctggg cggcgtccgc gacgcgcagt ggggaccggt cctgacggtg gggctcggcg
     5581 ggatctacgt cgaggtcgtg ggggataccg cggttgccct ggcgccggtc tctcccgccg
     5641 aggccaccgc gctcctcgac gagctccgga tcgcgcccct gctggaccgg cccgcgctgg
     5701 ccggcatcat cagccggttc tcgcacctgc tggtggatct ccccgagctg gccgagatcg
     5761 agatcaatcc cctgatgacg agcccagacg gcgcgatcgc gatggacgcg cgcgcccgcc
     5821 tcggcgcata ggagcgcagg gtggcgtccg gctacaaccg gatcatcgtg ggagtggacg
     5881 gctcgccgca ctcgcggcgg gcggtcgcgc tcgtggcgcg gctcgcgccg ccccgcggcg
     5941 ggcgggtcac gtgcgtgcgg gtgatcgagg cggtgcggct gccgtcgatg ccgttcgtgc
     6001 cggc
//
LOCUS       DATMCV010000106         4342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_538427, whole genome shotgun sequence.
ACCESSION   DATMCV010000106 DATMCV010000000
VERSION     DATMCV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538427"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VNU03_02605"
     CDS             <1..959
                     /locus_tag="VNU03_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HWP73368.1"
                     /translation="AERLSGPLAAFEAQAWPDDLRFLRERLAQVARQALELIEAFSAA
                     AGTGEPIGLYRALRRLGPLQEALYPLYPVLGPVSRWFLEAARRGDEDLVERLRVGALR
                     EDGVRGGILHAQNDRASRGGLSLYVPETSDGRKPASLIVALHGGSGHGRDFLWAWLRE
                     ARTRGALLLSPTAQDRTWSIMGGEDVDAPRLVAAVEEVAQRYPVDRARVLLTGMSDGA
                     TYALLAGLAEDTPFTHLAPACGVLHPYLLAEGGIHRARGRPIYLVHGALDWMFPVETA
                     RMARRALEGAGARVVYREIEDLSHTYPRDENPRILDWLGAAG"
     gene            complement(956..1729)
                     /locus_tag="VNU03_02610"
     CDS             complement(956..1729)
                     /locus_tag="VNU03_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73369.1"
                     /translation="MRTARRLLGETLIPAATLLAALLAWEVATYAFRIPRFIMPAPSA
                     ILGEGWEWRYRFIGHTWVTLYETLGGFALSMVVGVPLAVLIVYSPVLRAAIYPLVILA
                     QSVPKIAIAPVLLLVLGYGEVPKMVVAFLVAFFPVVVDTATGLAATPPELLDLSRSYR
                     ASAFKTFMKVRLPMALPFIFAGAKVAITLSVIGAVVGEFVGSDKGLGYVILSSTSYWK
                     TEVAFSAMILLSLMAIVLFAAVSLVERFVCPWLNPQGEG"
     gene            complement(1726..2520)
                     /locus_tag="VNU03_02615"
     CDS             complement(1726..2520)
                     /locus_tag="VNU03_02615"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73370.1"
                     /translation="MASPRPCPPRDPARLGREDLSHAHRRPGPRPRRDDPHGGENEFV
                     TLVGPSGCGKSTLLKLVAGLVPPTSGRIRVRDQEVREPFPDVGFVFQQPVLLPWRSVL
                     DNVLFSVEMLGLDPRQYRKPAGDLLELTGLAGFETKYPRELSGGMQQRVAICRALLPD
                     PSLLLMDEPFGALDALTREEMSLELLRIWEERRKTILFVTHSIPEAILLADRVVVMTP
                     RPGRVARMITVDLPRPRTMALEFDPRFKAYSDEVRGLIFSGRKERP"
     gene            complement(2489..3991)
                     /locus_tag="VNU03_02620"
     CDS             complement(2489..3991)
                     /locus_tag="VNU03_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWP73371.1"
                     /translation="MALLIRGGQVLAGTPATLTRADVLVEGDRIAAVGPGLAAPEGTP
                     VIDASGHIVLPGMGNAHTHAASHLARGRAASWTLEDLLTHSAANYGFRTPEDEYLSAA
                     VGAIEMLKTGCTSAYDLYMAVPAITDEAFEAVVRAYTDVGVRVVLAPAVADVVFYQTV
                     PGLADQLPADLRRTVEEMQPAPTKGLLGLTERAIRRWHGGADGRVRIGVAPTIPNQAT
                     DEFLDGCAALAREYGVGIHTHLAESKVQAVESQRRWGKSIVARLADHGILGPGFVGAH
                     SIWLTDDDLRMLADAGAAVAHNPGSNLRLGCGIAPVREMLDRGVAVGLGTDGSVCADN
                     QNLFEALRIASVISTIRFPHETTRWLDADTVWGLATSGTARVLGQAGDLGAVAPRRKA
                     DLVLLRADSVFLRPLADPVKALVYAETGGAIDTVLVDGRVVLERGRVTTVDEDRIYAQ
                     AQAAADRQRGQSAQAWALAERLAPFVAAACRGAVTTPLSINRFAAALPSA"
     gene            complement(4015..>4342)
                     /locus_tag="VNU03_02625"
     CDS             complement(4015..>4342)
                     /locus_tag="VNU03_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73372.1"
                     /translation="KDPEGLKAFLRATLRGADYAFRQKHFDEGVGYVVKSHPEVDPEG
                     AVGAAQVGARFVYASEVTSGKMAVGQFDPAQLEKTRDLYTQYLELKRKVGLEEIYTND
                     LLPAKK"
BASE COUNT          621 a         1539 c         1600 g          582 t
ORIGIN      
        1 acgccgagcg cctgtccggg ccgctggccg ccttcgaggc ccaggcgtgg cccgacgatc
       61 tgcgattcct gcgcgagcgg ctggcccagg tggcgcggca ggctctggag ctgatcgagg
      121 ctttcagcgc cgcggccggg accggcgagc cgatcgggct ctaccgcgcg ctgcggcgcc
      181 tcggcccgct gcaggaggcg ctctacccgc tctacccggt gctggggccg gtgagccgat
      241 ggttcctcga ggccgcccgc cgcggggacg aggatctcgt ggagcgcctg cgcgtgggcg
      301 cgctccgcga ggacggggtg cggggcggca tcctgcacgc gcagaacgat cgggccagcc
      361 gcggcgggct ctcgctctac gtgcccgaaa cgtcagatgg ccggaaaccg gcgtcgctga
      421 tcgtcgcgct ccacggcggc agcggccacg ggcgcgactt cctgtgggcc tggctccgtg
      481 aggcgcgcac ccgcggcgcg ctgctgctct cgcccaccgc ccaggatcgc acctggtcga
      541 tcatgggcgg cgaggacgtg gacgctccgc ggctcgtcgc cgcggtggag gaggtggcgc
      601 agcgctaccc ggtcgaccgc gcgcgggtgc tgctcaccgg catgtcggac ggcgccacct
      661 acgcgctgct ggccgggctc gcggaggaca cgccgttcac ccacctggcc ccggcctgcg
      721 gggtgctgca tccgtacctg ctggccgagg gcggcatcca ccgcgcgcgc gggcggccga
      781 tctacctggt ccacggcgcg ctcgactgga tgttcccggt ggagaccgcg cggatggcgc
      841 ggcgggcgct cgagggcgcg ggggcgcgcg tggtctaccg cgagatcgag gacctctccc
      901 acacctatcc gcgcgacgag aatccgcgga tcctcgactg gctgggagcc gcgggctagc
      961 cctcgccctg ggggttgagc caggggcaca cgaagcgctc cacgagggag accgccgcga
     1021 agaggacgat cgccatcagg gagagcagga tcatcgcgct gaacgcgacc tcggtcttcc
     1081 agtacgaggt ggacgagagg atcacgtagc cgaggccctt gtccgagccc acgaactcgc
     1141 ccaccaccgc gccgatcacc gagagggtga tcgcgacctt ggcccccgcg aagatgaacg
     1201 gcagggccat cggcaggcgc accttcatga aggtcttgaa cgcgctcgcg cggtaggagc
     1261 ggctcaggtc gagcagctcg ggcggggtgg ccgcgaggcc ggtggcggtg tccaccacca
     1321 ccgggaagaa cgccaccagg aaggccacca ccatcttcgg gacctcgcca tagcccagga
     1381 ccagcagcag caccggggcg atggcgatct tgggcaccga ctgggccagg atgaccagcg
     1441 ggtagatcgc ggcgcggagc accggcgagt agacgatgag caccgccagc ggcaccccca
     1501 cgaccatgga gagggcgaag ccgccgagcg tctcgtagag ggtcacccac gtgtggccga
     1561 tgaagcggta gcgccactcc cagccctcgc cgaggatggc cgacggcgcg ggcatgatga
     1621 agcgcgggat gcggaaggcg tacgtggcca cctcccacgc gagcagtgcc gcgagcaggg
     1681 tcgcggcggg aatgagggtc tcgcccagga gccggcgcgc cgtcctcacg gccgctcctt
     1741 ccggcccgag aagatcagcc cgcgcacctc gtcgctgtag gccttgaagc gcgggtcgaa
     1801 ctcgagggcc atggtgcgcg gccgggggag gtcgaccgtg atcatccgcg ccacgcgccc
     1861 gggccgcggg gtcatcacca ccacgcgatc cgccagcagg atggcctccg ggatcgaatg
     1921 ggtgacgaag aggatcgtct tgcgccgctc ctcccagatg cgcagcagct cgaggctcat
     1981 ctcctcccgg gtcagcgcgt cgagcgcccc gaagggctcg tccatcagga gcaggctcgg
     2041 gtcaggcagc agcgcgcggc agatggccac ccgctgctgc atgccgccgg agagctcgcg
     2101 cgggtacttg gtctcgaagc cggcgagccc ggtcagctcc aggagatccc ccgccggctt
     2161 gcggtactgg cgcggatcga ggccgagcat ctccaccgag aagaggacgt tgtcgagcac
     2221 gctgcgccag ggcaggagca ccggctgctg gaagacgaag ccgacgtccg ggaacggctc
     2281 gcgcacctcc tggtcccgca cccggatgcg gccgctggtc gggggcacga ggccggcgac
     2341 cagcttcagc agggtgctct tgccacagcc gctgggcccg accagggtga cgaactcgtt
     2401 ttccccaccg tgagggtcgt ctcggcgagg gcgtggacca ggtcgccggt gcgcgtgcga
     2461 taggtcttct cgaccgagtc gagccggatc acgcggaggg caaggccgcg gcgaagcgat
     2521 tgatggagag cggtgtggtc accgcgcccc ggcacgccgc cgcgacaaag ggcgccaggc
     2581 gctcggccag cgcccacgcc tgggcgctct gcccgcgctg gcgatcggcc gccgcctggg
     2641 cctgagcgta gatgcggtcc tcgtcgacgg tggtcacgcg gccacgctcc agcacgaccc
     2701 ggccgtccac cagcacggtg tcgatggccc cgccggtctc ggcgtagacc agcgccttga
     2761 ccgggtcggc cagggggcgc aggaagaccg agtccgcgcg tagcaggacc aggtcggcct
     2821 tgcgccgggg cgccaccgcg ccgagatcgc ccgcctggcc gagcacccgc gcggtgccgc
     2881 tggtggcgag gccccagacg gtgtccgcgt cgagccagcg ggtggtctcg tgcgggaagc
     2941 gaatcgtgct gatgacggag gcgatgcgga gggcctcgaa gaggttctgg ttgtcggcgc
     3001 agacggagcc gtcggtgccc aggcccaccg cgaccccgcg atcgagcatc tcgcgcaccg
     3061 gcgcgatccc gcagcccaga cgcaggttgc tgccgggatt gtgcgcgacg gccgcgcccg
     3121 cgtcggccag catgcgcaga tcgtcgtcgg tgagccagat cgagtgcgcg cccacgaagc
     3181 cgggcccgag gatgccgtgg tcggccaggc gggccacgat gctcttgccc cagcgccgct
     3241 ggctctccac cgcctgcacc ttggattcgg ccaggtgggt gtggatgccc acgccgtact
     3301 cgcgggccag cgccgcgcag ccgtcgagga attcgtcggt ggcctggttc gggatcgtcg
     3361 gcgcgacccc gatgcgcacg cgcccgtcgg ccccgccgtg ccaccggcgg atggcccgct
     3421 cggtgaggcc cagcagcccc ttcgtcggcg cgggctgcat ctcctcgacc gtccgccgca
     3481 ggtcggccgg cagctggtcg gccaggcccg ggacggtctg gtagaagacg acgtcggcga
     3541 ccgccggggc cagcaccacc cgcaccccca cgtcggtgta ggcgcggacc accgcctcga
     3601 aggcctcgtc ggtgatcgcc ggcaccgcca tgtagaggtc ataggccgac gtgcagccgg
     3661 tcttgagcat ctcgatggcc ccgacggcgg ccgagagata ctcgtcttcc ggcgtcctga
     3721 atccatagtt tgccgccgag tgggtgagca ggtcctcgag ggtccagctc gcagcccggc
     3781 cccgggccag gtggctcgcg gcgtgggtgt gggcgttgcc catgcccggc agcacgatgt
     3841 ggccggaggc gtcgatgacc ggggtgccct cgggcgcggc aagaccgggc cccaccgcgg
     3901 cgatgcgatc accctcgacc aggacgtccg cccgcgtgag cgtggccggc gtgccggcca
     3961 ggacctgccc tccccggatc agcagagcca tcggatcctc cgtgctcccg cgcactactt
     4021 cttcgcggga agcaggtcgt tggtgtagat ctcctcgagg ccgaccttcc gcttcagctc
     4081 gaggtactgg gtgtagagat cgcgggtctt ctcgagctgg gccggatcga actggccgac
     4141 cgccatcttg ccgctggtga cctcgctcgc gtacacgaag cgcgcgccca cctgggcggc
     4201 gcccaccgcg ccctccggat cgacctcggg gtgcgacttg accacgtagc ccacgccctc
     4261 gtcgaagtgc ttctgccgga acgcgtagtc ggcgcccctg agggtcgcgc gcaggaaggc
     4321 cttgagtccc tcggggtcct tg
//
LOCUS       DATMCV010000107         2463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_872124, whole genome shotgun sequence.
ACCESSION   DATMCV010000107 DATMCV010000000
VERSION     DATMCV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872124"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..710)
                     /locus_tag="VNU03_02630"
     CDS             complement(99..710)
                     /locus_tag="VNU03_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73373.1"
                     /translation="MPSSAGNEPISESSALHARAMADLRFIRETMESAAAFSAFSGWG
                     LILIGGVATVCGLVAARQPTHLGWLAAWVAAAGVSAVIGALSTARKTRTAQEPLIPGP
                     ARKFALSLAPAVVSGALLTGALERSGRFDLLPGMWLLLYGAGLVAAGAWSVRVVPVIG
                     TAFMVLGAVGLVLPLSWSNWLLIAGFGGLHVAFGVIVGRRHGG"
     gene            794..2185
                     /locus_tag="VNU03_02635"
     CDS             794..2185
                     /locus_tag="VNU03_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWP73374.1"
                     /translation="MPTPPAVERPNLLVILIDDLRFDEFGAGGHPYMLTPHVDRLARE
                     GAMCLNAFHTTPLCSPNRASIVTGQYASRHGIIDNVARDAASHRLPNYHLELQRLGYE
                     TAHVGKWHMGNDGSPRLGYDRWVSFDGHGKLFDPELNVDGQRRAVPGYVTDVLNAEAV
                     AFVEQRRAKPWSLFFAHKAVHPDAHQAADGTLDLSRGGYKPAERHRDLYRGCVFPPRP
                     NVLPPEEVVRAKPAWAEAFRLKATERAQAVLAAIHAGTQEEIRLRAAMMASVDEGVGM
                     LLDALERTGQLERTCILFLGDNGYFFGEHALGPERRFAYEEGIRSPFLVRYPPLVPAG
                     SQPEALVLALDIAPTMIALAGGTPGSHVQGRSLVPLLGGRRPADWRTSFLVEYWSENA
                     MPWLVGMTYKAVRTERAKLIHWVNRDGVDELYDLATDPYELANLIDDPARAREREDLR
                     RELARLVVEALGL"
     gene            complement(2190..>2463)
                     /locus_tag="VNU03_02640"
     CDS             complement(2190..>2463)
                     /locus_tag="VNU03_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HWP73375.1"
                     /translation="ACADSWGKALKTGEPALTIPCYAERRYGGVADDEMLMAIPPRFL
                     PKVIEGLAALSKNGLRYPIPPFGIQSDAGAGLAVSYPSPAAGAGGR"
BASE COUNT          369 a          892 c          887 g          315 t
ORIGIN      
        1 cggagcctcg gcctgcggcg ggccgcggtg cctggacccg aggcgactgg ccccgcggcg
       61 ccgaagcctc cgcccgctgc cgacgtgccg cgcccggatc agccaccgtg cctcctcccc
      121 acgatcaccc cgaaagcgac gtgcagccct ccgaagccgg cgatcaggag ccagttgctc
      181 cacgaaagcg gaagcaccag ccccaccgcg cccaggacca tgaaggcggt gccgatgacc
      241 ggaacgaccc tcaccgacca cgcgccggcc gcgacaagac cggccccgta gagcaggagc
      301 cacatcccgg gcagcagatc gaagcgcccg cttcgctcga gggcgccggt gaggagggct
      361 cccgagacga ccgcgggcgc caggctcagg gcgaactttc tcgccggccc cgggatcagc
      421 ggctcctgcg cggtgcgggt cttccgggcc gtcgagagag cgccgatcac cgccgagacg
      481 ccggcggcgg ccacccacgc cgcgagccag cccaggtggg tgggctgccg ggcggccacg
      541 agcccgcaca cggtcgcgac gccgccgatc aggatcaggc cccagccgga gaacgcggag
      601 aacgcggcgg cgctctccat ggtctcccgg atgaagcgga gatcggccat ggcccgggca
      661 tggagggccg acgactcgga gatcggctcg ttgccggcac tcgagggcat ggaatcatga
      721 atagcgtcgt tggttcagtc tgtcaagtac tttgaaatcc agagtcacgg accggccgtg
      781 ctaggctgcc ggcgtgccta cgcccccggc ggtcgagcgt ccgaaccttc tcgtcatcct
      841 gatcgacgac ctgcgcttcg acgagttcgg ggcgggcggg cacccgtaca tgctgacgcc
      901 ccacgtggac cggctcgccc gcgagggcgc gatgtgtctc aacgcgttcc acaccacgcc
      961 gctctgctcg cccaaccggg cgtccatcgt gaccggccag tacgcgagcc gccacggcat
     1021 catcgacaac gtggcgcgcg acgcggccag tcaccggctg ccgaactatc acctggagct
     1081 gcagcggctg ggctacgaga ccgcgcacgt tggcaagtgg cacatgggca acgacggctc
     1141 gccgcgcctg ggctacgacc gctgggtgag cttcgacggc cacggcaagc tcttcgatcc
     1201 cgagctgaac gtcgacggcc agcgccgggc cgttcccggg tacgtgaccg acgtgctgaa
     1261 cgcggaggcg gtcgcgttcg tggagcagcg gcgcgcgaag ccgtggagtc tcttcttcgc
     1321 ccacaaggcg gtgcacccgg acgcgcacca ggctgccgac ggcacgctcg atctgagccg
     1381 cggcggctac aagccggccg agcgccaccg ggacctctac cggggctgcg tgttcccgcc
     1441 gcggccgaac gtgctgccgc ccgaggaggt ggtgcgggcc aagccggcct gggcggaagc
     1501 cttccggctc aaggcgacgg agcgcgcgca ggcggtgctg gccgcgatcc acgcggggac
     1561 gcaggaggag atccgcctgc gcgccgcgat gatggcctcg gtggacgagg gggtcgggat
     1621 gctgctcgac gcgctcgagc gcaccgggca gctcgagcgc acgtgcatcc tcttcctggg
     1681 cgacaacggc tacttcttcg gcgagcacgc cctcgggccg gagcggcgct tcgcctatga
     1741 agaaggcatc cgctcgccct tcctggtgcg ctacccgccg ctcgtgccgg cggggagcca
     1801 gccggaggcg ctggtgctgg cgctcgacat cgcgcccacc atgatcgcgc tggcaggcgg
     1861 cacgccgggc tcgcacgtcc agggtcgctc gctggtcccc ctgctgggcg gccggcgccc
     1921 ggccgactgg cgcacctcgt tcctcgtcga gtactggagc gagaacgcga tgccctggct
     1981 ggtggggatg acctacaagg cggtgcggac cgagcgggcc aagctgatcc actgggtcaa
     2041 ccgcgacggg gtggacgagc tctacgacct cgcgacggat ccctacgagc tcgcgaacct
     2101 catcgacgat ccggcccggg cgagggagcg cgaggacctg cggcgagagc tcgcccggct
     2161 cgtggtcgag gcgctcggac tctgagccgt cagcggccgc ccgccccggc ggccgggctc
     2221 gggtagctga ccgcgagccc cgcccccgcg tcggactgga tgccgaaggg agggatcggg
     2281 tagcggagcc cgttcttgct cagcgcggcc aggccctcga tgaccttggg caggaagcgc
     2341 ggcgggatcg ccatcagcat ctcgtcgtcg gccaccccgc cgtagcggcg ctccgcgtag
     2401 caggggatgg tgagcgcggg ctcgccggtc ttgagcgcct tgccccacga gtccgcgcag
     2461 gcc
//
LOCUS       DATMCV010000108         2203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_538470, whole genome shotgun sequence.
ACCESSION   DATMCV010000108 DATMCV010000000
VERSION     DATMCV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538470"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..492
                     /locus_tag="VNU03_02645"
     CDS             211..492
                     /locus_tag="VNU03_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73376.1"
                     /translation="MESPTLGFLHITRGPGPIDPVNAEYRIVFAPLGGRLRKRHALCQ
                     GFDGLTDFLRQARVPLPEIERAWQNLARRRIHSVPRVALTRPQIDALGL"
     gene            complement(946..1343)
                     /gene="rnpB"
                     /locus_tag="VNU03_02650"
     ncRNA           complement(946..1343)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VNU03_02650"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1402..1878)
                     /locus_tag="VNU03_02655"
     CDS             complement(1402..1878)
                     /locus_tag="VNU03_02655"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HWP73377.1"
                     /translation="MRKRTTARRAGTKRAQAKRARTKRIPTKRAAPSPRKARSRTRKR
                     TPATRRGREGGEDRGQTIGLLRDLTEHHETGPAATGGDIDADWQRAQSSGEEAVGGSV
                     STPDQDIVDEIGHALGVEQPPAAPLRASEEILADRDQRYWDLERRANRKAVKRQEP"
     gene            1986..>2203
                     /locus_tag="VNU03_02660"
     CDS             1986..>2203
                     /locus_tag="VNU03_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73378.1"
                     /translation="MVSRIVLAWLLVAPGLVGELPAAVAGQGSAPVATLADGRVGQIH
                     FESRTPAGYFALARREAAPGAVVVGTLRL"
BASE COUNT          282 a          764 c          743 g          414 t
ORIGIN      
        1 cggcgaccca cgtgaactgg tgcggacaca cgggcaggag gtcatcccgc ccccattgcc
       61 acaccgccgc gtggcgttcg tgccggtgct gggggaggca aactcgaggc gggcgtcccg
      121 atcgcctaaa cgtttggcat atagcccggc cagcgcttcg cgaagtctcc atgggggtcc
      181 taccgatgca cacctctatg attgggctcc atggaaagcc cgaccctggg gtttctgcac
      241 atcacccgag ggcctggtcc catcgacccc gtcaacgccg agtaccggat cgtgttcgca
      301 ccactgggtg gtcggctgcg caagcggcat gccctgtgcc aaggcttcga cgggctcacc
      361 gatttcctcc gacaagcgcg cgtgcccctt ccagagattg aacgagcctg gcagaatctg
      421 gcaaggcgtc ggatccactc ggtcccgcgg gtcgcgctga cccggccgca gatcgacgcg
      481 ctcgggctct agcctcttcg gggttccaaa aggagacgag gcgatgggcg tctacgcgga
      541 agtccgaggg ttcgtcctcg ctcatcgtcc gtgcgccggc cctcgccacg ccaatgccgg
      601 gccgccgacc gtcaacggcc atcggttgat cgtcgtctgt gggtgctggc cgagttcagg
      661 cggtgggtca ctcttgatga tgccgacgaa gacttgctga ggtcggcgct gctagcgttc
      721 gagaactagg ggagaccctc cagctcctct caccgtcggg aggcgctcgg gcacgccgac
      781 atttgctcga ccatgggcta cgcggcgctc gcggacgacc gcctcgcgcg ggcgatcggg
      841 accctgacct gggatccggt tgttgcgaca tcgaacccgg ctggttatgt ctcaccggat
      901 tcgaggcaga tggggtaggg ggcaagtgtg cgagatcttt aaatgcgtca gagaaagacc
      961 gataagccgg gttctgtctg accgccgagg ttgcccccgt cggcgagatg atcatttctc
     1021 taggacgccg gttgcccggc gcctcaagcg gccatacccg agagcaccac gcgggccgcg
     1081 cttcacctgc tcttgcgagc aggatcgctc tcctatttgg ccttgctccg ggtggggttt
     1141 accgagccga cccggtcacc cggatcgctg gtgagctctt acctcaccgt ttcaccctta
     1201 ccgcccgtga aggcggcggt ctgttctctg tggcactgat ccgtggggtc gccccccctg
     1261 gccgttagcc agcaccctgc cctgcggagc ccggactttc ctcccgtgcg gttgcccgca
     1321 ccggcgatca tccagccttc tctgacgcaa tcaaagagcg tgtgtcgctg acgagcagta
     1381 tagcctaggc gccgagggtg ctcacggctc ctggcgcttg accgccttcc ggttggcgcg
     1441 ccgctccaga tcccagtagc gctggtcgcg atcggccagg atctcctccg aggcccgcag
     1501 cggcgccgcg ggtggctgct ccacgccgag cgcgtggccg atctcgtcga cgatgtcctg
     1561 atccggggtg gacacgctgc cgcccaccgc ttcctcgccc gagctctgcg cgcgctgcca
     1621 gtccgcgtcg atgtcgccgc cggtggctgc ggggccggtc tcgtggtgct cggtcagatc
     1681 gcggaggagt ccgatcgtct ggccgcggtc ctcgccgcct tcccgcccgc gccgggtggc
     1741 cggggtgcgc ttgcgcgtgc ggctgcgcgc cttccgggga gagggcgccg cgcgcttcgt
     1801 cggaatgcgc ttcgtccggg cccgcttcgc ctgcgcgcgc ttggtcccgg cgcgtctcgc
     1861 cgtggtgcgc tttctcacgg tccgcttcgc cgccgctcgt ctcggcctgg tcttgcgctt
     1921 cctcatgatc cacctcccgg gttgggcgat cccgacggct cgttctgtgg tagagaatcg
     1981 ttcgcatggt ctcacgaatc gtgctggcgt ggctgctggt ggcccccggt ctggtcgggg
     2041 agctcccggc tgccgtggcg gggcagggaa gcgcgccggt cgccaccctg gccgacggcc
     2101 gggtgggcca gatccacttc gagagccgga cccccgccgg ttacttcgct ctcgcccggc
     2161 gcgaggcggc gccgggtgca gtggtggtcg gcacgctccg cct
//
LOCUS       DATMCV010000109         5703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_938136, whole genome shotgun sequence.
ACCESSION   DATMCV010000109 DATMCV010000000
VERSION     DATMCV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938136"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="VNU03_02665"
     CDS             complement(<1..1983)
                     /locus_tag="VNU03_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP73379.1"
                     /translation="MYIVGIDVGGTFTDLTAVDATTGRVVVTKVPSRPRQEAAAVLAG
                     LEALGIASADVRRLVHGTTVGTNAVLERRGARVALLTTAGFRDLIEIGRTKRNIPALF
                     IPTFTRPKPVVERKDRFEVTERLGPDGAVLVPLEPASIERALDAALAAPAEAVAVCLL
                     HAYLNPAHEHAVADAVKGRAPGLPVSCSADVVAEYREFERFSTTVLNAYLQPLMEGYL
                     TSLEERLLATGYVHGVLTVASSGGMMTTDTARRLPIKTIFSGPAGGVSQACFVGAAAG
                     IRDFITYDMGGTSTDVCLVRDLQPLMTADAMVGAFPVKVSQIDMHTVGAGGGSIAWLD
                     VDGSLAVGPRSAGASPGPAAYGLGATEPAVTDANVVLGRIGTRRRLGGSISLDVERAR
                     TAVAALAARMERPLGVEALAEGIVTIAVARMTSAIREISIQRGHDPRDFTLIAFGGAG
                     PMHALAMADEIGIPRVLIPRHPGNFSALGLLAADIKHDDVRTRVGLLRERLPALRAAF
                     AEMETAARLQLEREGFAPEQQKLLRSLDLRYRGQAFELSVTVGPGAAEGEPSALQEIE
                     ADFHVHHRETYGHSNPQAAIELVNARLTAYGLVPKPAAERHTASGATLEGALVERRPV
                     WFGGQAHDCPVWDRDRLPESAQVRGPAIVEEFGATTV"
     gene            complement(1989..2945)
                     /locus_tag="VNU03_02670"
     CDS             complement(1989..2945)
                     /locus_tag="VNU03_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWP73380.1"
                     /translation="MLQFPNGSANLTYLLRVGTTELVLRRPPFGQIAPGAHDMKREFR
                     VLSRLWRHFDRSPRGYLLCDDPAVLGADFFVMERRRGEVIRDTMPPALQAHADVGRRV
                     AFALVAAMAELHALDPTACGLADLGRPDGFVDRQLDGWAKRWALARPDGADDTMERIH
                     ERLARRRPAPGRASIVHNDLKLDNCQFAPEDPDRVAAIFDWDMTTLGDPLVDLGTLLN
                     YWPDPTDTPATQRGTRPGLAHMGLPTRAEITERYAALSGTDPAAARWWEAFALWKTVV
                     VVVQLHRRWVRGESTDPRMAHIADRAPSLVTAAQLVLDSAGL"
     gene            complement(3128..3886)
                     /locus_tag="VNU03_02675"
     CDS             complement(3128..3886)
                     /locus_tag="VNU03_02675"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009719838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWP73381.1"
                     /translation="MLSHLFDLSGKVALVTGGSRGLGRAMVLAFAEAGADVVIASRKL
                     ASCEATAEEVRKATGRRALPVACHVGHWGQVEALAERAYAEFGRVDVLVNNAGMSPLY
                     DRIENVSEDLWDKVLDVNLKGPFRLTAMVGARMAAGAGGSIIMVSSTAAVRPSAGVIP
                     YAAAKAAVNALTEGFARAFGPTVRVNCIMPGPFLTDISKAWDLEAFQARAKETMALGR
                     GGEPDEIVGAALYFASGASSFTTGAVLGIHGGAA"
     gene            complement(3880..4596)
                     /locus_tag="VNU03_02680"
     CDS             complement(3880..4596)
                     /locus_tag="VNU03_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004553610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HWP73382.1"
                     /translation="MDPASISALTFDVFGTVVDWRSSITREGQAFGKHRGLSVDWAKF
                     ADAWRGLYQPAMEEVRSGRRPWVRLDDLHRESLVRLLRDFGIEGLSPAEIDDLNRAWH
                     RLDPWPDVVAGLTRLKRRYVLATLSNGNIALMVNMAKRAGLPWDTILGAEVAQAYKPT
                     PEAYLRSAEALGLRPEQCLMVAAHPGDLKAAARCGLRTAYVPRPLEHGPGRGADEPDT
                     AQPFDVVVGDFVELAERLEC"
     gene            4717..5034
                     /locus_tag="VNU03_02685"
     CDS             4717..5034
                     /locus_tag="VNU03_02685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWP73383.1"
                     /translation="MIAIWVKVRIKPDMREKFLKAIEVDALGSERDEPGCLRFNVLQD
                     EKDANVYYFFEVYKDQAALEAHRNTPHYAVWRGVADALEGPTEPTRCTTVFPAARAYW
                     GKM"
     gene            complement(5038..>5703)
                     /locus_tag="VNU03_02690"
     CDS             complement(5038..>5703)
                     /locus_tag="VNU03_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73384.1"
                     /translation="EAQRQHGTGQAARLLHGALLSGSGFLLESPRSERRLPIETTYSL
                     DQFLADTRTTIKTKGIPAGLAEIRDHLEKLLHNPELLKKHLGDPVPHVERTTIGHDPE
                     TDVHVLVHGRAKAAKSSVHDHGPCWVVYGNYQNATRMRRWRRLDDGSKPGHAELTLQR
                     EFMNEPGHAAAFAVGDIHSIEFGDDTFFIRVTGGDVETKETHRFDVEKKTVEVANRAI
                     GQR"
BASE COUNT          776 a         1948 c         2201 g          778 t
ORIGIN      
        1 gacggtggtc gcgccgaact cctcgacgat ggcgggcccc cgtacctggg cgctttcggg
       61 cagccgatcg cgatcccaga ccgggcagtc gtgggcctgg cccccgaacc agaccgggcg
      121 ccgctcgacc agggcgccct cgagggtcgc gccggatgcg gtgtggcgct ccgcggcggg
      181 cttgggcacg agcccgtagg cggtgagccg cgcgttgacc agctcgatgg cggcctgcgg
      241 gttcgagtgc ccgtaggtct cgcggtggtg gacgtggaaa tccgcttcaa tttcttgcaa
      301 tgcgctcggc tcgccttcgg ctgcacctgg cccaacagtc acgctcagct cgaaggcctg
      361 gccgcggtag cggagatcga gcgagcggag gagcttctgc tgctcgggcg cgaagccctc
      421 gcgctcgagc tggaggcggg cggctgtctc catctcggcg aaggcggcgc gcaaggcggg
      481 caggcgctcg cgcaggaggc ccacccgggt acggacgtcg tcgtgcttga tgtcggccgc
      541 gaggagcccc agggccgaga agttgcccgg gtggcggggg atgagcacgc gcgggatgcc
      601 gatctcgtcg gccatggcca gcgcgtgcat ggggccggcc ccgccgaagg cgatcagggt
      661 gaagtcgcgc gggtcgtggc cgcgctggat cgagatctcg cggatggccg aggtcatgcg
      721 agccaccgcg atggtgacga tgccctccgc gagcgcctcc acgccgagcg ggcgctccat
      781 gcgtgcggcg agggcggcca ccgcggtccg cgcccgctcg acgtcgagcg agatgctgcc
      841 gcccaggcgc cgccgggtgc cgatgcggcc caggaccacg ttggcgtcgg tgaccgcggg
      901 ctcggtggcg cccaggccgt aggcggcggg gccgggggag gcgcccgcgc tgcgcgggcc
      961 gaccgcgagg ctgccgtcca cgtcgagcca ggcgatggag ccgccacccg cgccgacggt
     1021 gtgcatgtcg atctgcgaga ccttgaccgg gaacgcgccc accatggcgt cggcggtcat
     1081 cagcggctgg agatcgcgca ccaggcagac gtcggtcgag gtgccgccca tgtcgtaggt
     1141 gatgaagtcg cggatgccgg ccgcggcgcc cacgaagcag gcctggctga ccccgccggc
     1201 cgggcccgag aagatggtct tgatgggcag ccggcgcgcg gtgtcggtgg tcatcatgcc
     1261 cccgctcgag gccaccgtga gcacgccgtg gacgtagccg gtggcgagca ggcgctcctc
     1321 cagcgaggtg aggtagccct ccatcagcgg ctggagatag gcgttgagca cggtggtgga
     1381 gaagcgctcg aactcgcggt actcggccac cacgtccgcc gagcaggaga cgggcaggcc
     1441 cggcgcgcgg cccttcaccg cgtcggccac cgcgtgctcg tgggccgggt tgaggtaggc
     1501 gtggagcagg cagaccgcca ccgcctcggc gggcgcggcc agcgccgcgt cgagcgcgcg
     1561 ctcgatggac gcgggctcga gcggcaccag caccgcgccg tcgggaccga gccgctcggt
     1621 cacctcgaag cggtccttgc gctcgaccac cggcttgggg cgggtgaagg tcgggatgaa
     1681 cagggccggg atgttgcgct tggtgcggcc gatctcgatc aggtcacgga agcccgcggt
     1741 ggtcagcagc gccacccgcg ccccgcgccg ctcgagcacc gcgttggtgc cgacggtggt
     1801 gccgtgcacg aggcggcgca cgtccgcgct ggcgatgccg agcgcctcga ggcccgccag
     1861 caccgcggcc gcctcctggc ggggccgcga cggcaccttg gtcacgacca cgcggccggt
     1921 ggtcgcgtcc accgcggtca gatcggtgaa ggtgccgccc acgtcgatgc cgacgatgta
     1981 cacggcggtc agagccccgc cgagtcgagc acgagctgcg ccgccgtcac cagcgagggc
     2041 gcccggtccg cgatgtgagc catgcgtgga tcggtggact cgccccgcac ccaccgccgg
     2101 tggagctgca cgaccaccac caccgtcttc cagagcgcga aggcttccca ccaccgcgcc
     2161 gcggccggat cggtgccgct gagcgcggcg tagcgctcgg tgatctcggc gcgggtgggc
     2221 aggcccatgt gggccaggcc cggccgcgtg ccgcgctggg tggcgggcgt gtcggtcggg
     2281 tcgggccagt agttcaggag cgtgcccaga tccacgagcg gatcgccgag cgtggtcatg
     2341 tcccagtcga agatggcggc cacgcggtcg gggtcctcgg gggcgaactg gcagttgtcg
     2401 agcttgaggt cgttgtggac gatcgacgcg cgcccgggcg cgggcctgcg gcgcgcgagc
     2461 cgctcgtgga tgcgctccat ggtgtcgtcg gcgccgtcgg ggcgggcgag ggcccagcgc
     2521 ttggcccagc cgtcgagctg gcggtcgacg aagccgtcgg ggcgtcccag gtccgcgagg
     2581 ccgcacgcgg tgggatcgag cgcgtgcagc tcggccatgg cggcgaccag cgcgaaggcg
     2641 acgcggcggc cgacgtcggc gtgcgcctgg agggccggcg gcatggtgtc gcgaatcacc
     2701 tcgccccggc gccgctccat gacgaagaag tcggccccga gcaccgcagg gtcgtcgcag
     2761 aggaggtagc ctcgcgggga gcggtcgaag tggcgccaga gccgcgagag gacccggaac
     2821 tcgcgcttca tgtcgtgggc gcccggcgcg atctgcccga acgggggccg gcgcagcacc
     2881 agctcggtgg tcccgacgcg gagcaggtag gtgaggttgg cggagccgtt ggggaactgc
     2941 agcacctcga tcgggccgcg cgcgtccggg agggcctcgg ccagtcgcgg gcgcagccac
     3001 gcctccagcg cggcccagtc gagcgcctct cccgggcgga ccggagcgat ctcggcgggc
     3061 gcgtcggaga cgggagctgc cgggacgtgc gcgccctccg cggccggggc gcgcggggag
     3121 cgggcgctca ggcggcgcct ccgtggatgc cgagcaccgc gccggtggtg aagctcgacg
     3181 cgcccgaggc gaagtagagc gcggccccga cgatctcgtc gggctcgccg ccgcggccga
     3241 gcgccatcgt ctccttggcg cgcgcctgga aggcttcgag gtcccacgcc ttcgagatgt
     3301 cggtgaggaa ggggcccggc atgatgcagt tcacccgcac cgtggggccg aacgcgcgcg
     3361 cgaagccctc ggtgagcgcg ttcaccgccg ccttggccgc cgcgtaggga atgacgccgg
     3421 cggagggccg caccgcggcg gtgctcgaca ccatgatgat cgagccgccc gcgcccgcgg
     3481 ccatccgcgc acccaccatc gcggtgagac ggaacgggcc cttcaggttc acgtcgagga
     3541 ccttgtccca caggtcctcg ctgacgttct cgatgcggtc gtagagcggg gacatccccg
     3601 cgttgttgac cagcacgtcg acccgcccga actcggcgta cgcgcgctcg gcgagggcct
     3661 cgacctggcc ccagtggccg acgtggcagg ccaccggcag ggcgcggcgg ccggtcgcct
     3721 tgcggacctc ctcggcggtc gcctcgcagg aggcgagctt gcggctggcg atcaccacgt
     3781 cggcgcccgc ctccgcgaag gccaggacca tggcgcgccc gaggccgcgg ctgccgccgg
     3841 tgaccagcgc gaccttgccg ctcaggtcga agaggtggct cagcattcca gccgctcggc
     3901 cagctccacg aagtcgccca ccaccacgtc gaacggctgt gcggtgtcgg gctcgtcggc
     3961 gccgcggccg gggccgtgct cgagcgggcg ggggacgtag gcggtgcgca gcccgcagcg
     4021 ggccgccgcc ttgaggtcgc cgggatgcgc cgcgaccatc aggcattgct cggggcgcaa
     4081 ccccagcgcc tcggccgagc gcaggtaggc ctcgggggtg ggcttgtagg cttgcgccac
     4141 ctccgcgccc aggatggtgt cccacggcag gccggcgcgc ttggccatgt tcaccatcag
     4201 cgcgatgttg ccgttcgaga gcgtggccag gacgtagcgg cgcttgaggc gggtgagacc
     4261 ggccaccacg tccggccacg ggtcgagccg gtgccaggcc cggttgagat cgtcgatctc
     4321 ggccggagag aggccctcga tcccgaagtc gcgcagcagg cggaccaggc tctcgcggtg
     4381 gaggtcgtcg aggcgcaccc acgggcggcg gccgctccgc acctcctcca tcgcgggctg
     4441 gtagagcccg cgccaggcgt cggcgaactt cgcccagtcc accgagaggc cgcggtgctt
     4501 accgaaggcc tggccttccc gggtgatgct gctccgccag tccaccaccg tgccgaagac
     4561 gtcgaaggtg agcgcgctga tcgaggcggg gtccatgaga tgggctcctt gtgtcgcgca
     4621 tgctacgacg gccgggacgg ggaaggcaag ccgcgggcag gcgtggtaat gtcggccgcg
     4681 gcggcgcagc gaccacttcg caggacggag gcgaccatga tcgcgatatg ggtgaaggtg
     4741 cggatcaagc cggacatgcg ggagaagttc ctgaaggcca tcgaggtgga cgcgctgggc
     4801 tcggagcgag acgagccggg ctgcctgcgc ttcaacgtgc tccaggacga gaaggacgcc
     4861 aacgtctact acttcttcga ggtatacaag gaccaggccg ccctggaggc ccaccgcaac
     4921 acgccgcact acgcggtctg gcgcggggtg gccgacgcgc tcgagggccc gaccgagccc
     4981 acccgctgca cgacggtgtt cccggccgcg cgcgcctact gggggaagat gtagcggcta
     5041 gcgctgcccg atcgcgcggt tggcgacctc caccgtcttc ttctccacgt cgaagcggtg
     5101 ggtctccttg gtctccacgt cgccgccggt gacccggatg aagaaggtgt cgtcgccgaa
     5161 ctcgatcgag tggatgtcgc ccaccgcgaa ggcggccgcg tggccgggct cgttcatgaa
     5221 ctcgcgctgc agggtgagct cggcgtgccc gggcttgctg ccgtcgtcca gccggcgcca
     5281 gcggcgcatg cgggtggcgt tctggtagtt gccgtagacg acccagcacg gtccgtggtc
     5341 gtgcaccgag gacttggccg ccttggcccg cccgtgcacg agcacgtgca cgtcggtctc
     5401 cggatcgtgg ccgatggtgg tccgctccac gtggggcacc gggtcgccca ggtgcttctt
     5461 caggagctcc gggttgtgga ggagcttctc caggtgatcg cggatctcgg cgagcccggc
     5521 cgggatgccc ttggtcttga tggtggtccg ggtgtcggcc aggaactgat cgagggagta
     5581 cgtcgtttcg attgggagtc tcctttccga acgaggggat tctagcagaa aacccgatcc
     5641 cgagaggagg gccccatgaa gaagacgagc cgcctggccg gtgccatgct ggcgctgagc
     5701 ctc
//
LOCUS       DATMCV010000110         6094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_470793, whole genome shotgun sequence.
ACCESSION   DATMCV010000110 DATMCV010000000
VERSION     DATMCV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470793"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VNU03_02695"
     CDS             complement(<1..203)
                     /locus_tag="VNU03_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73385.1"
                     /translation="MSAEAKIAWVERGGDALAIVMGLALRLAVPETSIVTTRPMLLVA
                     AGVVLLAVGLARDLARIALEGRPV"
     gene            460..681
                     /locus_tag="VNU03_02700"
     CDS             460..681
                     /locus_tag="VNU03_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73386.1"
                     /translation="MTQTEDVLAEYVERLFAKASLMSLAVLGHGRLLEDGCLRPDGNR
                     LEQLQSLATELEFGLGMLHDRLTERHELA"
     gene            698..1297
                     /locus_tag="VNU03_02705"
     CDS             698..1297
                     /locus_tag="VNU03_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWP73387.1"
                     /translation="MTAIGRVTLALCVLAPLAVGCARHVDAPQPGAVTASRPAASPAP
                     PVWPDAAPAPDAPVPDAAGPTPESGPTAGFSAREELPDIHFATGQVHAEPSARKALDA
                     AAAWLKTHPEQLVIVEGYTDVAGPRLANLALAQRRAKWVMDYLVGKGIAPSRITAVSR
                     GESGALCADRSAACQGKNRRVHFLVRDTGSVQVTASPSR"
     gene            complement(1275..2237)
                     /locus_tag="VNU03_02710"
     CDS             complement(1275..2237)
                     /locus_tag="VNU03_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWP73388.1"
                     /translation="MTSSSIRSRLAAFSLVGLVLGPAAPASAACGPSPLPADAPIALV
                     LSGGGAKGAYEAGVAAALVERGVPIRLAAGSSAGALNAAMIADGRLDRLEALWRGLTR
                     DRVYSLRTRVFFAGLLPGWLTLLTLDRAGSLFDPEPLRELIGASLDLERIRVSPVRLL
                     VVTTDLQRREPRVFDNRTVTREVLMAAAAVPGAFPAVDVGGTLLVDGGLTGRAPVLEA
                     LASGTAVERAVVVMSYAPQEQGSPPTTMRRALEEAFEMGMIHQIRRDVELARFKFPGV
                     EVELLTPSAPLALRPLEFDPAGMARALERGRADAVACLSGWGKR"
     gene            complement(2288..2692)
                     /locus_tag="VNU03_02715"
     CDS             complement(2288..2692)
                     /locus_tag="VNU03_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73389.1"
                     /translation="MRTILVVDDDAWVCGLARDVLAREGYRVLEASDGQDAIRVSAEH
                     PGPIHLLLTDVMMPGMNGCDLAAGLSALLPGLKVMFMSAYDRDFLVARGLNPVGPVIT
                     KPFTIEYLTRRVQMVLGDRRAAAPVATAAPAR"
     gene            complement(2698..3036)
                     /locus_tag="VNU03_02720"
     CDS             complement(2698..3036)
                     /locus_tag="VNU03_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73390.1"
                     /translation="MTPEKDTFRIELRNAKLCMELDCNTVFDAVTHRHCPTCSSAEFY
                     PLESWLNRDRRERREPTIREQRTTVRAAISALPRPLWLDRLYVPRESESEMAGASVRP
                     RRAAQRRRAG"
     gene            3376..4683
                     /locus_tag="VNU03_02725"
     CDS             3376..4683
                     /locus_tag="VNU03_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP73391.1"
                     /translation="MDANEPTDLTVSEASRRIADGALTPSALLEACLQRIAATDDAVH
                     AWVSVDEPGARATARERTDEARAGRRRGPLHGIPVAIKDIFHVAGMTTTCGAAPAFHT
                     AATADATSVARLRAAGAVVLGKVHTTEFAYFEPGPTRNPWNPAHTPGGSSSGSAAAVG
                     ARMAPLALGTQTVGSVLRPAAYCGVVGFKGTYDLIPTEGVVPLAWSFDHVGVFARRVA
                     DVVLAVDVLAARPLGAPAVSAPRVAVAEELLERATPEVAALIRATADRLARAGATVHE
                     VTLPPSFAGVHAAGRSVLEVEAAAYHEELYRAHAAQYRPRTRELIASGLTRPAVVYAR
                     AQRARLAFRRDVIELLAEYDALLSPTAPSPAPEGLGATGDPWFCAPWSFAGVPAVSLP
                     IGVSSLGLPHAVQLVGAAGHDAKILAVAAWCERVVSFELAPSR"
     gene            4863..>6094
                     /locus_tag="VNU03_02730"
     CDS             4863..>6094
                     /locus_tag="VNU03_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWP73392.1"
                     /translation="MIGADVLPGTARTQPWRARLRELVIRRSLLLSVVAVALVLLALV
                     EMRTSALQALVLSQAARVLTYRLAPGPSPSVSYPQTGPYDVRLGYTRLPDFVDRLEAG
                     GYRVEQQARPSAALAHLAAWGFYPPYREKTRAGLEVLDREGHPLYATRYPEQVYDRFD
                     AVPPLLINTLLFIENRELLDTGPYRNPVVDWRRLAKAVGVETIGRFRGERHSIGASTL
                     PTQLEKMRHSPEGRTRAPGDKLRQMISASLRAYQAGATTRATRRQIVVDYLDSLPLAA
                     APGHGEVHGLGDGLRIWYGADFLQANALLSTKPAAADAARARVFKRALSLILAARRPY
                     YYLVENPRALEEFTDGYLRVLARAGVIDARLRDAALGERLELRRGGAAAPVAFVERKG
                     ANLVRARLTSMLSVPALY"
BASE COUNT          807 a         2271 c         2194 g          822 t
ORIGIN      
        1 accgggcggc cctcgagcgc gatgcgcgcg aggtcgcggg cgagcccgac cgcgagcagc
       61 accacgccgg cggcgacgag cagcatcggc cgcgtggtca cgatgctggt ctccggcacg
      121 gccagccgga gcgcgagccc catgacgatg gcgagagcgt ctccgccccg ctcgacccac
      181 gcgatctttg cttctgcgct catctgcggc ccccttgtga gcccgcaccc ggtggcgggc
      241 tcctcccatc gtggcacggc gggcgggccc ggcatgtccg gtctctgacg ggccgagcgg
      301 aacgctgtcg tggcgctgac gagccaccgg cgcgatcgtg gcctaaaatt ccaattttca
      361 taggtacttg aacgactcag cccgtggcac ggcgtatgcg ctatgtggcc catcgccggc
      421 cggttggaac ggccgagcgc ccgagaaagg gagcgaccca tgacccagac cgaggacgtg
      481 ctggcggagt acgtggagcg gctgttcgcg aaggcgagtc tcatgagtct cgcggtgctc
      541 ggacatggcc gcctgctgga agacggctgc ctgcgccccg acggtaaccg gctcgagcag
      601 ctgcagtccc tggccaccga gctcgagttc ggcctcggga tgctgcacga tcggctgacc
      661 gagcgccacg agctggcctg attccgcgcg cgccccgatg accgcgatcg gacgcgtgac
      721 tctcgccctc tgcgtactgg cgccgctggc cgtcggctgc gcccggcacg tggacgcgcc
      781 gcaacccggc gcggtcaccg cgagtcggcc cgccgcatct cccgccccgc cggtctggcc
      841 ggacgccgcc cccgctcccg atgcgcccgt cccggatgcg gcgggtccca cccccgagtc
      901 cggtcccacc gccgggttct ccgcccgtga ggagctgccg gacatccact tcgcgaccgg
      961 ccaggtccac gcggagccga gcgcgcggaa ggcgctcgac gcggcggcgg cgtggctcaa
     1021 gacgcacccc gagcagctcg tgatcgtgga gggctacacc gacgtggccg ggccccgcct
     1081 cgccaacctc gccctggcgc agcggcgggc gaagtgggtg atggactacc tcgtgggcaa
     1141 gggcatcgcg ccgagccgca tcaccgcggt gtcccgcggc gagagcggcg cgctgtgcgc
     1201 cgaccgcagc gcggcctgcc agggcaagaa tcgccgcgtg cacttcctgg tccgcgacac
     1261 cgggtcggtg caggtcaccg cttcccccag ccgctgaggc aggccaccgc gtcggcgcgc
     1321 ccgcgctcga gggcgcgcgc catgccggcg ggatcgaact cgagcgggcg cagcgcaagc
     1381 ggcgccgacg gcgtcagcag ctccacctcc acccccggga acttgaagcg cgccagctcc
     1441 acgtcccgcc ggatctggtg gatcatcccc atctcgaagg cctcctcgag ggcgcgccgc
     1501 atcgtggtgg gtgggctccc ctgttcctgc ggcgcgtagc tcatcaccac caccgcccgc
     1561 tccaccgcgg tccccgacgc gagcgcctcc agcaccggcg cccgcccggt gagcccgccg
     1621 tccaccagca gcgtcccgcc gacgtccacc gcggggaagg cgccgggcac cgcggccgcg
     1681 gccatgagca cctcgcgggt gacggtccgg ttgtcgaaga cccgcggctc gcggcgctgg
     1741 aggtcggtgg tgacgaccag gagccgcacc ggcgagacgc ggatgcgctc gaggtcgagc
     1801 gaggcgccga tcagctcgcg gagcggctcg ggatcgaaga gcgagcccgc ccggtcgagc
     1861 gtcagcaggg tgagccatcc gggcaggagg cccgcgaaga acacgcgcgt ccgcagcgag
     1921 tagacccggt cgcgggtgag cccgcgccac agggcctcga gccgatcgag ccggccgtcg
     1981 gcgatcatgg ccgcattcag cgcgccggcc gacgagccgg ccgccaggcg gatcggcacg
     2041 ccccgctcca cgagcgcggc ggccacgcct gcctcgtagg cacccttggc cccgccgccc
     2101 gacagcacga gcgcgatcgg ggcgtcggcg ggaagcgggg acggtccgca cgcggcggag
     2161 gcgggagcgg ccgggcccag gacgagcccg acgagcgaga aggcggcgag acgtgagcgg
     2221 atcgacgaag acgtcatggc ccggctctta caacgagccg ggccatgacg tcaatgcgag
     2281 cggcgggcta gcgcgcgggc gcggcggtgg cgaccggtgc ggcggcgcgt cgatcgccca
     2341 gcaccatctg gacccggcgg gtcaggtact cgatggtgaa cggcttggtg atcaccgggc
     2401 cgaccggatt gaggccccgg gccaccagga agtcgcggtc gtaggcggac atgaacatga
     2461 ccttgaggcc gggcaggagc gcgctgagcc cggccgcgag atcacagccg ttcatgcccg
     2521 gcatcatcac gtcggtgagg agcagatgga tgggaccggg atgctcggcg gagacccgga
     2581 tcgcgtcctg cccgtccgag gcctcgagca cccggtaccc ctcacgggcc agcacgtcgc
     2641 gcgcgaggcc gcacacccac gcatcgtcgt cgacgaccag tatcgttcgc atgggcgtca
     2701 acccgcccgc cggcgctgcg cggcgcgccg cggccgcacg gaggctccgg ccatctcgct
     2761 ctcgctctcg cgcgggacgt acagccgatc gagccagagc gggcgcggga gagcggagat
     2821 cgccgcgcgc accgtggtcc gctgctcgcg gatcgtaggc tctcgccgct cgcggcggtc
     2881 gcggttgagc caggactcga gcgggtagaa ttcggcgctg ctgcaggtcg ggcagtgacg
     2941 atgtgtcacc gcgtcgaaga ccgtgttgca gtcgagctcc atgcagagct tggcattgcg
     3001 tagttcgatg cggaacgtgt ccttctcggg tgtcatcggg tcccatcctc ccgtgcgttc
     3061 ggtgtgacca ggcaggccga aggccggatg ctcggtccga cgcgtcggtg gagaagggac
     3121 cgaaaccccg tcccgctcac ccgcccctcg aacctgcctg atccgtaccg cagtaccggg
     3181 ggcaagtcct gtaccaagga ccgatcgctc gtagaatcaa tcgcttgctc gcgggcgccc
     3241 gcgccgagct tccctcgtgg cttccgtggc tgggtaacga aatgccctct tcgtatggaa
     3301 cgcgaatcca ccgcactttc tgcggtgtgt cgtcgtcttg acagcgatcg ggcgcgcccc
     3361 gcatgatcct tcgccatgga cgcgaacgag ccaaccgatc tcacggtctc cgaggcctcg
     3421 cgccgcatcg cggacggcgc gctcacgccg tccgcgctgc tggaggcgtg tctgcagcgc
     3481 atcgccgcga cggacgacgc ggtgcacgcg tgggtctcgg tggacgagcc gggcgcgcgc
     3541 gcgaccgcac gcgagcgcac cgacgaggcg cgcgcgggcc gccggcgcgg gccgctgcac
     3601 gggatcccgg tcgcgatcaa ggacatcttc cacgtggcgg gcatgaccac gacctgcggc
     3661 gccgcgcccg ccttccacac cgcggccacc gcagacgcca cgtcggtggc gcgcctgcgc
     3721 gcggcgggcg ccgtcgtgct cggcaaggtc cacaccaccg agttcgccta cttcgagccc
     3781 gggcccacgc gcaatccgtg gaatccggcc cacacgccgg gcggctcgtc ctccggctcc
     3841 gcggcggcgg tgggtgcccg catggccccc ctcgccctgg gcacccagac ggtgggctcg
     3901 gtgctgcggc ccgcggccta ctgcggcgtg gtgggattca agggcaccta cgacctgatc
     3961 ccaaccgagg gcgtggtccc gctcgcgtgg agcttcgatc acgtgggtgt cttcgcgcgc
     4021 cgcgtggccg acgtggtgct cgcggtggac gtgctggccg cgcgcccgct cggcgctccc
     4081 gcggtctccg cgccgcgcgt cgcggtggcg gaggagctcc tcgagcgcgc cactcccgag
     4141 gtggccgcgc tcatccgcgc caccgcggac cggctcgcgc gcgcgggcgc gacggtgcac
     4201 gaggtgacgc tgccgccgtc gttcgcgggc gtgcacgcgg cgggtcgctc ggtgctggag
     4261 gtcgaggcgg ccgcgtatca cgaggagctg taccgggccc acgccgcgca gtaccggccg
     4321 cgcacccgcg agctcatcgc gagcggtctc acgcgcccgg cggtggtcta cgcccgcgcg
     4381 cagcgggcgc ggctcgcgtt ccgtcgcgac gtgatcgagc tgctcgccga atacgacgcg
     4441 ctgctgtcgc cgaccgcgcc gtcgcccgcg ccggaagggc tgggcgcgac cggcgatccg
     4501 tggttctgcg cgccgtggag cttcgcgggc gttcccgcgg tctcgctgcc gatcggcgtc
     4561 tcgtcgctcg gtctccccca cgcggtgcag ctcgtcggcg cggcgggcca tgacgcaaaa
     4621 attctcgcgg tggccgcatg gtgcgagcgc gtggtaagct ttgagctcgc accatcgcgg
     4681 tgatggtgat cgctcccaat gcccacgacg caagcattgc atagaggaac gtccggcccg
     4741 accggacggt tgatcaggca gtggccgacg ccagcagccg ctcttcgctg agacgattcc
     4801 tctcggtgtc tcgatctgcc ccgcggtccg gcttggcacc cgggcaaccg gcgctggcgg
     4861 ccgtgatcgg ggccgacgtg ctcccgggta ccgcgaggac ccagccgtgg cgcgcgcgcc
     4921 tgcgcgagct ggtgatccgc cggtcgctgc tcctgtccgt cgtggcggtc gcgctcgtcc
     4981 tgctcgcgct cgtggagatg cgcacctccg cgctgcaggc cctggtgctc tcgcaggccg
     5041 cgcgcgtgct cacctaccgg ttggccccgg gcccgagccc gtcggtcagc tatccgcaga
     5101 ccgggcccta cgacgtgagg ctcggctaca cccggctccc cgacttcgtg gaccgcctgg
     5161 aggcgggcgg ctatcgcgtg gagcagcagg cccggccctc cgccgcgctg gcgcacctgg
     5221 ccgcgtgggg cttctacccg ccctatcgcg agaagacccg ggcggggctc gaggtcctcg
     5281 accgcgaggg ccacccgctc tacgcgaccc gctatccgga gcaggtctac gaccgcttcg
     5341 acgcggtgcc gcccctcctg atcaacaccc tgctcttcat cgagaatcgc gagctgctcg
     5401 acaccggccc gtaccggaac ccggtggtgg actggcgacg cctcgcgaag gcggtcgggg
     5461 tggagacgat cggccgcttc cgcggcgagc gccactcgat cggggcgagc accctgccca
     5521 cccagctcga gaagatgcgc cattctcccg agggccgcac ccgcgccccg ggggacaagc
     5581 tgcggcagat gatctcggcg agcctccggg cctaccaggc gggcgcgacg acgcgcgcca
     5641 cccgtcggca gatcgtggtg gactatctcg attcgctgcc cctcgccgcc gcgccggggc
     5701 acggcgaggt gcacggcctg ggcgacggcc tccgcatctg gtacggggcg gacttcctcc
     5761 aggccaacgc gctcctctcg accaagccgg cggccgcgga cgccgcgcgc gcccgcgtct
     5821 tcaagcgcgc gctgagcctc atcctcgccg cgcgccggcc ctactactac ctggtggaga
     5881 atccgcgcgc tctcgaggag ttcaccgacg gctacctgcg ggtgctggcc cgcgcggggg
     5941 tcatcgacgc gcgcctgcgc gacgcggccc tcggcgagcg gctcgagctg cggcgcgggg
     6001 gcgccgccgc gccggtggcg ttcgtggagc gcaagggcgc caacctggtc cgggcccggc
     6061 tcacctcgat gctgagcgtg cccgccctct acga
//
LOCUS       DATMCV010000111         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_6204, whole genome shotgun sequence.
ACCESSION   DATMCV010000111 DATMCV010000000
VERSION     DATMCV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6204"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VNU03_02735"
     CDS             <1..363
                     /locus_tag="VNU03_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HWP73393.1"
                     /translation="IIDIMLVSADQPRWPGLVEPIKSLDHVYWAENPRSDRMFFVKGM
                     PPFGTRRTHHVHVRTPEDGHPALVFRDYLRDHPDAVARYARLKRELEARHPTDREAYT
                     GGKDGFVAEIVRAATAGA"
     gene            complement(360..1091)
                     /locus_tag="VNU03_02740"
     CDS             complement(360..1091)
                     /locus_tag="VNU03_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWP73394.1"
                     /translation="MAKSAVTAERFAKGMTFDEYVTYAGSPQNLAREAFGSYFPDGGA
                     KGIARKDNSAVLRERYARTRLGDDQVAAIKWLAAQPGGPANILVISEDWSSDCRRDVP
                     ALARLAEAGGLALRIFNRDGQKILGQRRPDPAAYPDGNHDLMLELMNAKDGAEWASVP
                     AVGVYTKDFQELCRYLEFPAIYHKDLVRGHQQGARTGESPEQARDRGMKEFAAMQASP
                     FFDLWASAGIDEILSAIYEKRVLGG"
     gene            1147..1389
                     /locus_tag="VNU03_02745"
     CDS             1147..1389
                     /locus_tag="VNU03_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HWP73395.1"
                     /translation="MLELRPGCECCDRDLPPDSDQARVCSFECTFCRACAETVLHDRC
                     PNCGGELVPRPRRPADKLAKFPASATRVHKPAGCRG"
     gene            1488..1859
                     /locus_tag="VNU03_02750"
                     /pseudo
     CDS             1488..1859
                     /locus_tag="VNU03_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1864..2505)
                     /locus_tag="VNU03_02755"
     CDS             complement(1864..2505)
                     /locus_tag="VNU03_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWP73396.1"
                     /translation="MTTVLVAARGAARARLEAVVGAARALRLLAGQPEAPLARRLQAL
                     RPDVVLLDLDGGRLDAVLGELPAAAAAALVVLADAPRRVRAAVAMERGLVRAVLPRDA
                     NSGEILAAIDAVVAGLVVLHPDALADALAGRPRREALPARAVADPAQPLTAREIEVLG
                     MLAEGLGNKTIAVRLGISTHTAKFHVASILAKLGAGSRTEAVTVGMRRGLVVI"
     gene            complement(2510..>2869)
                     /locus_tag="VNU03_02760"
     CDS             complement(2510..>2869)
                     /locus_tag="VNU03_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWP73397.1"
                     /translation="RGARLVARDPRRDLAALVVDATGLPAAPIGDSAALRVGELVLAV
                     GNPLGLVRALSAGLVHAVGPRAIHADLRLAPGNSGGPLADARGRVVGLNAMIVGGLAV
                     AVPSREAQRFVREQLRG"
BASE COUNT          378 a         1125 c          998 g          368 t
ORIGIN      
        1 atcatcgaca tcatgctggt ctcggccgac cagcctcgct ggccggggct ggtcgaaccc
       61 atcaagagcc tcgaccacgt ctactgggcg gagaatccgc ggagcgaccg catgttcttc
      121 gtgaagggca tgcccccgtt cggcacccgc cgcacgcatc acgtccacgt ccggacaccc
      181 gaggacgggc acccggcgct cgtctttcgc gactacctcc gcgatcaccc cgacgcggtc
      241 gcccgctacg cgcggctgaa gcgcgagctc gaagcccgcc accccaccga tcgggaggcg
      301 tacaccgggg gcaaggacgg attcgtcgcg gagatcgtgc gggccgcgac ggcgggcgcc
      361 tagccgccca gcacccgctt ctcgtagatc gcgctcagga tctcgtcgat ccccgcgctc
      421 gcccacaggt cgaagaaggg cgacgcctgc atcgcggcga actccttcat gccgcggtcg
      481 cgggcctgct cggggctctc cccggtccgc gccccctgct gatggccacg cacgaggtcc
      541 ttgtggtaga tcgcgggaaa ctcgaggtag cggcacagct cctggaagtc cttggtgtag
      601 acccccaccg cgggcaccga ggcccactcc gcgccgtcct tggcgttcat cagctcgagc
      661 atgaggtcgt gattgccgtc cgggtacgcg gccgggtccg gccggcgctg gccgaggatc
      721 ttctggccgt cgcgattgaa gatgcgcaat gcgagcccgc ccgcctccgc gagccgcgcc
      781 agcgccggca cgtcgcgccg gcagtccgac gaccagtcct cggagatcac caggatgttg
      841 gcggggccgc ccggctgggc cgcgagccac ttgatggcgg cgacctggtc gtcgcccagg
      901 cgcgtccggg cgtagcgctc gcgcagcacc gcgctgttgt ccttgcgcgc gatgcccttc
      961 gcgccgccgt cggggaagta gctcccgaag gcctcgcgcg cgaggttctg ggggctgccc
     1021 gcgtacgtca cgtactcgtc gaacgtcatg cccttcgcga atcgctccgc cgtcaccgcg
     1081 ctcttggcca tggtctcctc ctcgggttcc agatgatact ctgatggcca ccgaggaggg
     1141 agtcccatgc tggagctgcg accgggctgt gaatgctgtg accgcgacct gccgccggac
     1201 tccgaccagg cgcgcgtgtg ctcgttcgag tgcaccttct gtcgcgcgtg cgccgagacc
     1261 gtgctccacg accgctgtcc caactgcggc ggcgagctgg tgccgcgccc gcggcggccc
     1321 gcggacaagc tggcgaagtt cccggcgtcc gccacgcggg tgcacaagcc cgcgggctgc
     1381 cggggttgat ccgcgcgtgc gtccatccca gcgccctgcg ggcgatcgag gtaccggatg
     1441 cctgacgctc cgccgctgtt ccgccgcgcg cgggccgagg acgtgcccgc gagcgcgacg
     1501 agacgccgct gccgccgtcc tatctggagg ccttcgccgc catcgaccgc gatcccaaca
     1561 acgagctgat ggtcgcctgc cgggacgcgg cggtggtcgg ggtcctccag ctgaccttca
     1621 tgccctcgat cacctaccag ggcggctggc gcgcccagat cgagggggtg cgcgtcgacg
     1681 cgcgggagcg ctcgcacggc ctcgggcgca cgctctttca gtgggccatc gcgcgggcgc
     1741 gcgagcgcgg gtgccacctg gtgcagctca ccaccgacaa gacgcggccg gacgcccggc
     1801 gcttctacga gggcctgggc ttcgtggcct cccacgaggg catgaagctc cacctctagc
     1861 cggtcagatc accaccagcc cgcgccgcat cccgaccgtc accgcctcgg tgcggctgcc
     1921 cgcgccgagc ttggccagga tcgacgccac gtggaacttc gcggtgtgcg tcgagatgcc
     1981 gagccgaacc gcgatggtct tgttgccgag cccctcggcc agcatgccca gcacctcgat
     2041 ctcgcgcgcg gtcagcggct gggccggatc ggcgacggcc cgcgcgggca gcgcctccct
     2101 ccgcggccgg ccggccaacg catccgccag cgcgtccgga tgcagcacca cgaggcccgc
     2161 gaccaccgcg tcgatggcgg ccaggatctc gcccgagttg gcgtcgcgcg gcagcaccgc
     2221 gcggacgagg ccgcgctcca tcgcgacagc ggcccgcacc cgccgcggcg cgtccgccag
     2281 caccacgagc gcggcggccg cggccgccgg cagctcgccc aggacggcgt cgaggcgccc
     2341 gccgtcgaga tcgagcagca ccacgtccgg gcgcagcgcc tgcagccggc gtgcgagcgg
     2401 agcctccggc tgccccgcga gcaggcgcag ggcacgcgcg gcgccgacca ccgcctcgag
     2461 ccgggcccgc gcggccccgc gcgccgccac cagcaccgtg gtcacgccct cagcctcgca
     2521 gctgctcccg gacgaagcgc tgcgcctcgc ggctcggcac cgccaccgcg agcccgccca
     2581 cgatcatcgc gttgagcccg accacacggc cccgcgcgtc ggcgagcggg ccgccggagt
     2641 tgccgggggc caggcgcagg tcggcgtgga tcgcccgcgg cccgaccgcg tgcacgaggc
     2701 cggccgagag cgcgcgcacc agcccgagcg gattgccgac cgccagcacc agctcgccga
     2761 cccgcagcgc ggccgagtcg ccgatcggag ccgccgggag cccggtcgcg tccaccacca
     2821 gcgcggccag gtcgcgccgg gggtcgcgcg cgaccagccg cgcgccccg
//
LOCUS       DATMCV010000112         3315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_605966, whole genome shotgun sequence.
ACCESSION   DATMCV010000112 DATMCV010000000
VERSION     DATMCV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605966"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VNU03_02765"
     CDS             <1..859
                     /locus_tag="VNU03_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWP73398.1"
                     /translation="PPRGQALPGRVAQGDISVIGPMARSAEDLAIGLTAMAGADEIDG
                     AGWRLDLPAPRRKTLREFKVAVMLTDPAAEVDREVQARLQALADFLGKKKAKVSHAAR
                     PDIDTAEANRAYIFLLRAATAGRQTPDEFRRNAEVTQGLAPDDESYYARMMRANTASH
                     RDWLAANETRHKMRWRWAEFFRDWDLLLCPVASSAAFPHDHEGERYERTIAVNRRRVP
                     AVDQLFWAGYSGMAYLPSTVAPCGFTPAGLPVGVQIVGPQYGDRTCIEFARLLEREFQ
                     AFVPPPDYR"
     gene            868..1785
                     /locus_tag="VNU03_02770"
     CDS             868..1785
                     /locus_tag="VNU03_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP73399.1"
                     /translation="MAEGSTTSWWQRLVSVTKALFWLVLIVVVLQQVYGSNKVENARA
                     SVLDRFQQERKSRVIALIHRQETVTLFGVPVSSSISIEDSEAVLRAIRLTPPEQPIDV
                     ILHTPGGLVLAAEQIAKALVQRKAKVTAFVPHYAMSGGSLIALAADEIVMDPNAVLGP
                     VDPQIGDMPAASIIKLLSLKKPAEISDEMLILADMSAKARVQVGVFVMEVLLKHMSKE
                     KAVALATTLTEGRWTHDFPITVEMARGLGLPIATEMPRTVYDLMDLYPQGGRGRPSVL
                     YVPLRPGMPERGESTTPSPGPPVKGRSWR"
     gene            1810..2682
                     /locus_tag="VNU03_02775"
     CDS             1810..2682
                     /locus_tag="VNU03_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP73400.1"
                     /translation="MAKRRVVITGAAGYVAQRMYPELAERWDLVQIDVRPTTRAGKAV
                     PGLIVSDLTRPDRNLYREHFRGADAVIHCGFVSAPNLDATTWQDNSDAKFWAEHANVA
                     LAYNVSRVALEEGVRRVVVCSSNHAADYYERLVWDGRLDMVTPEMPPRSDNWYGWAKA
                     AYELLGFVFATGKVDGRRLEVVQWRIGGPRDDDIDQVKPGDVKVMHRSLGAYLSQRDQ
                     VQQAIRMVETADIRDEHGVPFLIVYGISGNTHRFWSIANARTKIGYEPQDDSQVNFAD
                     KIAAIARAARKDKP"
     gene            2679..2876
                     /locus_tag="VNU03_02780"
     CDS             2679..2876
                     /locus_tag="VNU03_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73401.1"
                     /translation="MTHEDELRSLESTLAVYKETIKEVNRLGGDGMVSVRTEWLLRIT
                     ELELQREHLSSVVRRARRAAL"
     gene            3138..>3315
                     /locus_tag="VNU03_02785"
     CDS             3138..>3315
                     /locus_tag="VNU03_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWP73402.1"
                     /translation="MEVSVELRFVQVDVFTETVFGGNPLAVVFEAGGLDEGVMQRIAR
                     EMNCSETTFLLAPTR"
BASE COUNT          508 a         1109 c         1215 g          483 t
ORIGIN      
        1 gcccccgcgc ggccaggcgc tgcccggccg cgtcgcccag ggcgacatct cggtgatcgg
       61 cccgatggcg cggagcgcgg aggatctcgc gatcggcctc accgccatgg cgggggccga
      121 cgagatcgac ggcgcgggct ggcgcctcga tctgcccgcg ccccgccgga agacgctgcg
      181 cgagttcaag gtggcggtca tgctcaccga tcccgccgcg gaggtggatc gcgaggtgca
      241 ggcacgcctg caggccctcg ccgacttcct cggcaagaag aaggcgaagg tgagccacgc
      301 cgcgcggccc gacatcgata ccgcggaggc gaaccgggcc tacatcttcc tgctgcgcgc
      361 cgccaccgcg ggccggcaga cgcccgacga gttccggcgc aacgcggagg tcacgcaagg
      421 gctcgccccg gacgacgaga gctactacgc gcggatgatg cgggccaaca ccgcctcgca
      481 ccgggactgg ctcgcggcca acgagacgcg gcacaagatg cgctggcgct gggccgagtt
      541 cttccgcgac tgggacctgc tgctctgccc ggtggcctcc tccgccgcgt tcccgcacga
      601 tcacgagggc gagcgctacg agcggaccat cgcggtcaac cgccgccgcg tacccgcggt
      661 cgaccagctc ttctgggccg gctacagcgg catggcctac ctgccgtcca cggtggcgcc
      721 gtgcggattc acccccgccg ggctgccggt cggcgtgcag atcgtgggac cgcagtacgg
      781 cgaccggacg tgcatcgagt tcgcgcggct gctcgagcgc gagttccagg ccttcgtgcc
      841 cccgccggac taccgctgag cgagtcgatg gccgaaggat cgacgacctc gtggtggcag
      901 cggctggtca gcgtgaccaa ggcgctgttc tggctcgtgc tgatcgtggt cgtgctccag
      961 caggtctacg gctccaacaa ggtggagaac gcgcgggcgt cggtgctgga tcgcttccag
     1021 caggagcgca agtcgcgcgt catcgcgctc atccaccgcc aggagacggt gaccctcttc
     1081 ggggtgccgg tgagcagctc gatcagcatc gaggactcgg aggcggtgct gcgggccatc
     1141 cggctcaccc cgccggagca gcccatcgac gtgatcctgc acacgccggg cggcctggtg
     1201 ctcgccgccg agcagatcgc caaggcgctg gtgcagcgca aggccaaggt gaccgccttc
     1261 gtgccgcact acgcgatgtc gggcggctcg ctcatcgcgc tggccgccga cgagatcgtg
     1321 atggacccga acgcggtgct gggcccggtc gatccccaga tcggcgacat gccggccgcg
     1381 tccatcatca agctcctctc tctcaagaag ccggcggaga tcagcgacga gatgctgatc
     1441 ctggccgaca tgtcggccaa ggcgcgggtg caggtcgggg tcttcgtgat ggaggtgctg
     1501 ctcaagcaca tgtccaagga gaaggcggtg gccctcgcca ccacgctcac cgaggggcgg
     1561 tggacccacg acttcccgat cacggtggag atggcgcgcg ggctcggcct gcccatcgcc
     1621 accgagatgc cgcgcacggt gtacgacctg atggacctct atccgcaggg gggtcgcggc
     1681 cgcccctccg tgctgtacgt tccgctccgt cccggcatgc cggagcgggg cgagtcgacc
     1741 acgccgtccc ccgggccgcc ggtcaagggc cggtcgtggc gatgacgcga gtcgaggaga
     1801 cctgagccga tggccaagcg ccgtgtggtg atcaccgggg cggcgggcta cgtcgcccag
     1861 cggatgtatc ccgagctcgc cgagcgctgg gacctggtgc agatcgacgt gcggcccacc
     1921 acccgcgcgg gcaaggcggt gccgggcctg atcgtgagcg acctgacgcg ccccgatcgg
     1981 aatctctatc gcgagcactt ccgaggcgcg gacgcggtga tccactgcgg cttcgtgagc
     2041 gcccccaacc tcgacgcgac cacctggcag gacaacagcg acgccaagtt ctgggccgag
     2101 cacgccaacg tggcgctcgc ctacaatgtg agccgggtcg cgctggagga aggggtccgg
     2161 cgcgtggtgg tgtgctcgtc caaccacgcg gccgactact acgagcgcct ggtgtgggac
     2221 ggccgcctcg acatggtgac ccccgagatg ccgccgcgct cggacaactg gtacggctgg
     2281 gccaaggccg cctacgagct cctgggcttc gtgttcgcca ccggcaaggt ggacggccgg
     2341 cggctcgagg tcgtgcagtg gcgcatcggg ggtccgcgcg acgacgatat cgaccaggtg
     2401 aagccgggag acgtcaaggt catgcaccgg tcgctcgggg cctatctctc gcagcgcgat
     2461 caggtgcagc aggcgatccg catggtcgag accgcggaca tccgcgacga gcacggggtg
     2521 ccgttcctca tcgtctacgg catcagcggc aacacccatc gcttctggtc catcgccaac
     2581 gcccgcacca agatcggcta cgagccgcag gacgacagcc aggtgaactt cgccgacaag
     2641 atcgcggcca tcgcccgcgc cgcacggaag gacaagccat gacccacgag gacgagctcc
     2701 gatcgctgga gagcacgctg gccgtctaca aggagacgat caaggaggtc aaccggctgg
     2761 gcggggacgg catggtcagt gtgcgcaccg agtggctcct gcggatcacg gagcttgagc
     2821 tgcagcgtga acacctgagc tcggtggtgc ggcgggctcg gcgggcggcg ctgtagtcgg
     2881 cttgctccgc gggcagcggg ccgggagcgg ggggcggcgg gggcctcccc ggggcacgct
     2941 gagtcgccgg cgggttgcgc cgtctcgagc gggccacgag atggggcgtt ggcggcttcg
     3001 atgacccgca gaagccacgg ccccggggag gcccccgccg ccccccgctc ccggccctcc
     3061 gttcgtggtc gggtcgacag caggcggtcc gtgcggccga caccgggggt tgttccaact
     3121 cttgaaactt gcggctcatg gaggtgtcgg tggagttgag gtttgtgcag gtggatgtgt
     3181 ttacggagac ggtgtttggg gggaatccgc ttgcggtggt gtttgaggcg ggcgggctgg
     3241 atgagggggt gatgcagagg attgcgcggg agatgaattg ttcggagacg acgttcttgt
     3301 tggcgccgac gcggg
//
LOCUS       DATMCV010000113         2309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_272377, whole genome shotgun sequence.
ACCESSION   DATMCV010000113 DATMCV010000000
VERSION     DATMCV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272377"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VNU03_02790"
     CDS             complement(<1..266)
                     /locus_tag="VNU03_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73403.1"
                     /translation="MAGLPAVLDRLWRRWRLSRRDVVALGVASRGIWTTGERRALARR
                     LRRFARRVVVISDVEAAYLGALGGTAGVLLLAGTGSIALGRDRRG"
     gene            516..1124
                     /locus_tag="VNU03_02795"
     CDS             516..1124
                     /locus_tag="VNU03_02795"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73404.1"
                     /translation="MRRALMNLTAALLLSLIAATAAQAMTPTETVKSRVDEALQGLSQ
                     TTSATPESAERRRTEIRRAADSLFDFTEMSRRALGRHWADRTAAERDEFVRLFTDLVA
                     RSYIGKIDRYAGEAIAYTGERVEGDEASVRSQVVTAKGSQIPVEYRLHKTNENWTAYD
                     VFIENVSLIGTYRSQFDRIIKTESFGDLLRRLREKGQVADAR"
     gene            complement(1137..2096)
                     /locus_tag="VNU03_02800"
     CDS             complement(1137..2096)
                     /locus_tag="VNU03_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73405.1"
                     /translation="MMSLTIRLLLLLLLAAPLAPALPARAQTTETAAVITELKPGRGR
                     VEVKPAAGGDWKPARPLLALRAGDAVRTSDNASAVVLLSGGRGTVRIEGASGSFTVPG
                     AGAAGQGQKARALVEASLGFLAGTTKESSQAVLATRSAARPPIIVSPRNGRVLPETLA
                     FEWLGSRFGRYSVRVIGPAGPVVERREVTGARWEYPADAPALVPGMRYTLQVTAGGYP
                     PQEAWFELVDAERAREVAGDLAGLEQALGPSVSPTSRAVLRAGLLAREGFMHDARRVV
                     VTALAADRDEPTLHLLLGDLYTRTGLIDLAAESFDEAQYLTNK"
     gene            complement(2098..>2309)
                     /locus_tag="VNU03_02805"
     CDS             complement(2098..>2309)
                     /locus_tag="VNU03_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP73406.1"
                     /translation="KKYAVLGDTVNLTSRMEGLNRDLGTGILISGAVLAVLRDRMVVR
                     DRGSVAVKGRSEPVEIHELLSLEEA"
BASE COUNT          350 a          863 c          800 g          296 t
ORIGIN      
        1 ccgcggcgat cgcggccgag cgcgatggag ccggtgcccg cgagcaggag cacgcccgcg
       61 gtgcctccga gcgcgcccag gtaggcggcc tcgacgtcgg agatcaccac cacgcggcga
      121 gcgaatcgac ggagtcgccg cgcgagcgcg cggcgctcgc cggtcgtcca gatgccgcgc
      181 gaggccacgc cgagcgccac cacgtcacgg cgcgagaggc gccagcgacg ccagaggcgg
      241 tcgagcaccg cgggcagtcc cgccacgccc ggcgtcggcg cccggaagac tcggcgcccg
      301 ggtcccgacg cgaccacccg gacccacgtg ccgccgagat ccacaacgac gacgggtcgg
      361 ccggatccgc tcgttgcaag accgcgattc atgggagcta ttgtcccccg gcgggcatcc
      421 aaaacgaaag cacggtatcg tgggaataca cccccgctga acgcgtccta tcggacagca
      481 gcgtgattcg ttgagaccct gatcgaggag gcattatgcg acgtgccctg atgaacctga
      541 cggcggccct gctcctgagc ctgatcgcgg cgaccgccgc gcaggcgatg acgccgaccg
      601 agaccgtgaa gtcccgagtg gacgaggccc tccagggcct cagccagacc acgagtgcca
      661 cccccgagtc agccgagcgc cgtcgcaccg agatccgccg cgcggccgat tcgctcttcg
      721 acttcaccga gatgtcccgt cgcgcgctcg gccgccactg ggccgaccgc accgccgcgg
      781 agcgcgacga gttcgtgcgg ctcttcaccg atctggtcgc ccgctcgtac atcggcaaga
      841 tcgaccgcta cgcgggcgag gccatcgcct acaccgggga gcgcgtggag ggcgacgagg
      901 cgagcgtgcg atcgcaggtc gtgaccgcga agggctcgca gatcccggtc gagtatcgcc
      961 tgcacaagac caacgagaac tggaccgcct acgacgtctt catcgagaac gtgagcctga
     1021 tcggcaccta tcgcagccag ttcgaccgga tcatcaagac cgagtcgttc ggggacctgc
     1081 tgcgccgcct gcgcgagaag gggcaggtcg ccgacgcgcg gtagcccctg agggttctac
     1141 ttgttggtga ggtactgggc ctcgtcgaag gattcggcgg cgaggtcgat gagcccggtg
     1201 cgggtataga ggtcgcccag cagcaggtgc agggtgggct cgtcccgatc cgccgcgagc
     1261 gcggtcacca ccacccgccg cgcgtcgtgc atgaacccct cgcgcgcgag gagcccggcc
     1321 cgcagcaccg cgcgcgaggt cggtgacacc gaggggccca gcgcctgctc gaggccggcc
     1381 aggtctcccg cgacctcgcg ggcccgctcc gcgtccacca gctcgaacca cgcttcctgc
     1441 ggcgggtagc cgcccgcggt gacctgcagg gtgtagcgca tgccggggac gagcgcgggg
     1501 gcgtcggccg gatactccca gcgagcgccg gtgacctcgc gccgctcgac cacggggccg
     1561 gcgggaccga tcacgcgcac cgagtagcga ccgaagcggc tgccgagcca ctcgaacgcg
     1621 agggtctccg gcagcacgcg cccgttgcgc gggctcacga tgatcggcgg ccgcgccgcg
     1681 ctgcgggtgg ccagcaccgc ctgcgacgac tccttggtgg tcccggctag gaagccgagg
     1741 ctcgcctcca cgagcgccct ggccttctgg ccctggcccg ccgccccggc gcccggcacc
     1801 gtgaagctcc cgctcgcccc ctcgatccgc accgtgccgc gtccgccgga gagcagcacg
     1861 accgcgctcg cgttgtcgct ggtgcgcacc gcgtcgccgg cccgcagggc gagcagcggg
     1921 cgcgccggct tccagtcgcc gcccgctgcc ggcttgacct ccacgcggcc gcgtcccggc
     1981 ttcagctcgg tgatgaccgc ggcggtctcc gtcgtctggg cgcgggcggg gagggcgggc
     2041 gcgagagggg ccgccagcaa caggagtagc agcagtctta tcgtcaggct catcacgtca
     2101 cgcctcctcg aggctcagca gctcgtggat ctcgaccggc tcgctccgcc ccttcaccgc
     2161 cacgctgccc cggtcccgaa ctaccatccg gtcacgcagc acggccagta ccgcgccgct
     2221 gatcagaatg ccggtgccga ggtcgcgatt caacccctcc atgcgggagg tcaggttgac
     2281 ggtgtcgccc agcaccgcgt acttcttct
//
LOCUS       DATMCV010000114         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_606207, whole genome shotgun sequence.
ACCESSION   DATMCV010000114 DATMCV010000000
VERSION     DATMCV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606207"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..2503)
                     /locus_tag="VNU03_02810"
     CDS             complement(53..2503)
                     /locus_tag="VNU03_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP73407.1"
                     /translation="MKKDRFSIVGQSLPKIDAWAKVVGETKFADDLLMSRMAHGKLLR
                     SPHPHALIKRIDTTRARALPGVYAVITGHDLPRVKFGILPVSQDEEALCVDKVRMVGD
                     AVAAVAAVDEETAERATELIDVEYEPLRPLMSIEDSLAHPEVRIHEYGDRPNVHKAVA
                     LQFGDVEAAFGAADLVREDVFFFEGNTHLAMEQHSAVAQWGADGKLTLWSSTQTPHYV
                     HRLLAKILDLPQAHIRVVAAPVGGGFGGKLDPFAHEIAACKLSQLCGRPVKITLTREE
                     VFYVHRGRHPVLMWVKTGFTKDGAITGMHFRSWLDGGAYGSYGVASTFYTGALQTVTY
                     KIPVYKFEGARVFTNKPPCGPKRGHGTPQPRFAMECQIDKAAEQLGLDPADMRRRNLA
                     EPFVKTANHLTVTTIGLGECIDRVVEASGWREKRRTLPPGKGIGIACSSYLTGAGTAI
                     YWNDMPHSGVVVRADRSGLVAVLCGATDIGQGSDSILAYLVAEVLGIEPKDIRVHPAD
                     TDLTPVDLGSYSSRVTLMAGNAAIQAAERLRARIFEAVARKLEVTPDKLVAADRRVFV
                     DGDPDHGVDFAQAVVLAESMFGVLAFPGSYAPPKRAGKYKGGGVGPSPCYSYSACVVE
                     VNVDRDTGEVKPTEIWIAHDVGRALNPLLVEGQVEGSIYMGLGEVLMEEQVFRKGVHK
                     IPSLLEYKSPTTLETPEIHTILVETDDPEGPFGAKEAGQGPLLPVIPAVANAVYDAVG
                     VRIDETPITAEKVVKAMDSKRLGKTARLGPERLPVFTFPEAKVVESAFGQPAEAVAVR
                     PFATPHPALSPEGRGGSS"
     gene            complement(2500..3024)
                     /locus_tag="VNU03_02815"
     CDS             complement(2500..3024)
                     /locus_tag="VNU03_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWP73408.1"
                     /translation="MKTLLALRINGEERDVLAAPHKTLLEVLREELNLTGTKHGCELG
                     ECGTCTVLVDGLPVLSCLALPVECQGREIKTVEGMAQGGELHPLQQAFAELGAAQCGY
                     CTPGILLTAEALLVEIPTPTRDEIKHALSGNLCRCTGYTKILDAVELAALRMQRRGEV
                     QPQASRADTRTART"
     gene            3172..>3370
                     /locus_tag="VNU03_02820"
     CDS             3172..>3370
                     /locus_tag="VNU03_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73409.1"
                     /translation="MGIRRSHGKNKKSNQPTDAATVEREDILRRAANVRSRFDSTRRG
                     PGAAADRPAPGEPSSPDEGSAP"
BASE COUNT          506 a         1203 c         1151 g          510 t
ORIGIN      
        1 accgcgtcgg ccaccgacac cggcgccagg tacgagaacg gcggcaaccg catcatgacg
       61 agccccctct cccctctggg gagagggcag ggtgaggagt cgcgaaaggc ctcaccgcga
      121 cggcctccgc gggctggccg aaggccgact ctacgacctt cgcctccggg aacgtgaaca
      181 ccggcagtcg ctccggcccg aggcgcgccg tcttcccgag acgcttgctg tccatcgcct
      241 tcacgacctt ctcggcggtg atcggcgtct cgtcgatccg cacgccgacc gcgtcgtaga
      301 ccgcgttggc gaccgccggg atcaccggca gcaacggccc ctgcccggcc tccttggcgc
      361 cgaacggccc ctccgggtcg tcggtctcca ccaggatcgt gtggatctcc ggcgtctcga
      421 gcgtggtcgg gctcttgtac tcgaggagcg acgggatctt gtgcacgccc ttgcggaaga
      481 cctgctcctc catcagcacc tcgccgagcc ccatgtagat cgagccctcg acctggcctt
      541 ccaccagcag cgggttcagg gcccggccca cgtcgtgggc gatccagatc tcggtgggct
      601 tcacctctcc ggtgtcgcgg tccacgttca cctcgaccac acacgccgag taggaatagc
      661 agggcgaggg cccgacgccg ccgcccttgt acttgccggc gcgcttgggc ggcgcgtagg
      721 agccgggaaa cgccaggacc ccgaacatgc tctcggccag caccaccgcc tgggcgaagt
      781 ccaccccgtg gtcggggtcg ccgtccacga agacgcggcg gtcggccgcc accagcttgt
      841 cgggcgtcac ctcgagcttg cgggccaccg cctcgaagat cctcgcccgc agccgctccg
      901 cggcctggat cgccgcgttg cccgccatga gcgtcacccg cgaggagtag gagccgagat
      961 ccaccggggt gaggtcggta tcggccggat gcacgcggat gtccttcggc tcgatgccga
     1021 gcacctccgc gaccaggtag gccaggatcg agtccgagcc ctggccgatg tcggtggccc
     1081 cgcagagcac cgccaccagg ccgctgcgat cagcgcgcac caccacgccc gagtgcggca
     1141 tgtcgttcca gtagatcgca gtccccgcgc cggtgaggta cgacgagcac gcgatgccga
     1201 tccccttgcc cggcggcaag gtacgccgct tctcgcgcca gccggaggcc tcgaccacgc
     1261 ggtcgatgca ctcgccgagg ccgatggtgg tcacggtcag gtggttcgcg gtcttcacga
     1321 acggctcggc caggttgcgc cgccgcatgt cggccgggtc gaggccgagc tgttcggccg
     1381 ccttgtcgat ctggcattcc atcgcgaagc ggggctgcgg cgtcccgtgc ccgcgcttgg
     1441 ggccgcacgg cggcttgttg gtgaacacgc gcgcgccctc gaacttgtag accgggatct
     1501 tgtaggtcac ggtctggagc gcgccggtat agaaggtgga ggccaccccg taggagccgt
     1561 aggccccgcc gtcgagccag gagcggaagt gcatgccggt gatggcgccg tccttcgtga
     1621 agccggtctt gacccacatc agcaccgggt ggcgcccgcg gtggacgtag aagacctcct
     1681 cgcgggtcag cgtgatcttc accgggcggc cgcagagctg cgagagcttg cacgccgcga
     1741 tctcgtgcgc gaacgggtcg agcttgccgc cgaagccgcc gcccaccggc gcggccacca
     1801 cccggatgtg ggcctgcggc aggtcgagga tcttggcgag cagccggtgc acgtagtggg
     1861 gcgtctgggt cgaggaccag agggtcagct tgccgtccgc cccccactgg gccaccgccg
     1921 agtgctgctc catcgccagg tgggtgttgc cctcgaagaa gaagacgtcc tcgcggacca
     1981 ggtcggccgc gccgaacgcc gcctccacgt cgccgaactg gagcgccacc gccttgtgca
     2041 cgttcggccg gtcaccgtac tcgtggatcc gcacctccgg atgggccagc gagtcctcga
     2101 tggacatgag cggccggagc ggctcgtact ccacgtcgat cagctcggtc gcgcgctccg
     2161 cggtctcctc gtccaccgcg gccaccgcgg ccaccgcgtc gccgaccatg cgcaccttgt
     2221 cgacgcagag cgcctcctcg tcctgggaca ccggcaggat accgaacttc acccggggca
     2281 gatcgtggcc ggtgatgacc gcgtagacgc cgggcagggc ccgggcgcgc gtggtgtcga
     2341 tgcgcttgat cagcgcgtgg gggtgcgggc tgcgcaggag cttgccgtgg gccatgcgcg
     2401 acatcagcag gtcgtcggcg aacttggtct cgccgaccac cttggcccag gcgtcgatct
     2461 tgggcagcga ctggccgacg atcgagaagc gatccttttt catgtgcggg ccgtccttgt
     2521 atccgctcgg ctcgcctgcg gctgcacctc gccccgccgc tgcatccgca aagcggcgag
     2581 ctcgaccgcg tcgaggatct tggtgtatcc cgtgcagcga cacaggttgc ccgagagggc
     2641 gtgcttgatc tcgtctcggg tcggcgtggg gatctccacc agcagagctt cggcggtcag
     2701 gaggatgccg ggggtgcagt agccgcactg ggcggcgccg agctccgcga aggcctgctg
     2761 cagagggtgc agctcgccac cctgcgccat gccctcgacc gtcttgatct cccgcccctg
     2821 gcactcgacg ggcagcgcca ggcacgagag caccggcagg ccatccacca gcacggtgca
     2881 cgtgccgcac tcgcccagct cgcagccgtg cttggtgccg gtgaggttca gctcctcccg
     2941 caagacctcg agcagggtct tgtgcggcgc cgcgaggacg tcgcgctctt ccccgttgat
     3001 cctgagggcc aggagtgtct tcatgggtga ttacgtgagg atgaaactgg acttaagtaa
     3061 tactaaatca ggtcgtcccc tgtcaagcgc gggcccgccc ccccatggcc ctccggtgga
     3121 cctaaggtcc aatagccacc cgcgacccga agcctagact gggaagcacc gatgggaatc
     3181 cggcgcagcc acgggaagaa caagaagagc aaccagccca cggacgccgc gaccgtcgag
     3241 cgggaggaca tcctgcgccg ggcggccaac gtgcgcagcc gcttcgacag cacccggcgc
     3301 gggcccggtg cggcggcaga ccggccggcc ccgggcgagc cgtcgagccc ggatgagggc
     3361 tccgcgccag
//
LOCUS       DATMCV010000115         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_272552, whole genome shotgun sequence.
ACCESSION   DATMCV010000115 DATMCV010000000
VERSION     DATMCV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272552"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VNU03_02825"
     CDS             complement(<1..791)
                     /locus_tag="VNU03_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73410.1"
                     /translation="MRLLALALHRLLWFVPTLLGLLVVTFVISRVIPADPVALVAGET
                     ATPAQVEALRRQLGYDRPLPVQFVDYVARLARGDMGVSLFTRRPIVDDLAHRLPATIE
                     LTVVAMLASVLVGIPLGVVSALWRNSLLDHALRVLTVSGLAIAGFWLGIMLQLLFSMR
                     LGWTPLNGRLPGYPPRDLTGFYLIDAALTWDWGTFGAALQHLALPAATLAFPALATLV
                     RFTRAGVLDVMESNFALYERAMGLPRPLIVWKYVLRSDYNAVMGFT"
     gene            complement(823..>2339)
                     /locus_tag="VNU03_02830"
     CDS             complement(823..>2339)
                     /locus_tag="VNU03_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73411.1"
                     /translation="AAIPPALATAQTRFVFSNESPYDTMDPHAAFDVGRVAVRLNLYD
                     GLYRWVDNPPKLEPWLAESHTVSPDGLTYTFKLRHGAKFHDGAEITAEDVRYSAERIL
                     AMKKGAAALISTMIAPGATKALDRYTVQFTLTKPAAIFLAVVPEVHVVNSALLKKNEK
                     DGDLGAAWLTNNEAGSGSYMLTRYDPAIGFVAKRFPGHFMPWGAKFIEEIDFRFVKED
                     NTRVLGMIKGDYQGTGGYLPNDQVKRLREASNVKLVEAESMRIMMFQINNQRAPLTDV
                     HVRRAISYAFDYDGFNKEILGGTVERNPVPIPNNMWGVPRDVKGYTYSLDKAKQELGL
                     AQAKVDRPLTVGYLQGFSQTEQAATVMANGLRKLGLDTKLTSEVWPAMVERMKKPETS
                     PDLVVYWISTYYADPNNWIGEMFHSGQWGTFKASSFYKNPKVDDLLDAALKSTDRAVR
                     EKAYQEAARIVVNDAAGVWIYNTKYFGPWAKNIEGIRFSPIGNGQEIRWLYYSK"
BASE COUNT          365 a          747 c          843 g          384 t
ORIGIN      
        1 gtgaagccca tcaccgcgtt gtagtccgag cgcaggacgt acttccacac gatgagcggg
       61 cgcggcaatc ccatcgcgcg ctcgtagagg gcgaagttcg actccatgac gtccaggact
      121 ccggcccggg tgaaccgcac cagcgtggcc agcgccggga acgcgagcgt cgccgcgggc
      181 agggcgaggt gctgcagggc ggcgccgaag gtgccccagt cccacgtcag cgcggcgtcg
      241 atcaggtaga agccggtgag gtcgcgcggg ggatagccgg gcaggcggcc gttcagcggc
      301 gtccacccga ggcgcatcga gaagaggagc tgcagcatga tgcccagcca gaagccggcg
      361 atggcgagcc cggacacggt gaggacgcgc agggcgtggt cgagcaggga gttgcgccag
      421 agcgccgaga cgacgccgag ggggatgccg acgaggaccg aggcgagcat ggccaccacg
      481 gtcagctcga tggtggccgg gagccggtgc gcgaggtcgt cgacgatcgg gcgccgggtg
      541 aagaggctca cgcccatgtc cccgcgcgcg agccgggcga cgtagtcgac gaactgcacg
      601 ggcagcgggc ggtcgtagcc gagctggcgc cggagcgcct ccacctgggc cggggtggcc
      661 gtctctcccg ccaccagggc caccgggtcg gccgggatca cccgcgagat cacgaacgtg
      721 acgacgagca gcccgagcag ggtggggacg aaccacagga gccggtgcag cgcgagggcg
      781 aggagcctca tggctcagcg cccgcgcggc accgggtgac ggctatttcg agtagtacag
      841 ccagcggatc tcctgtccgt tgccgatggg actgaagcgg atgccctcga tgttcttggc
      901 ccagggcccg aagtacttgg tgttgtagat ccagacgccc gcggcgtcat tcaccacgat
      961 ccgggccgcc tcctggtagg ccttctcgcg aacggcgcga tccgtcgact tgagcgcggc
     1021 gtccaggagg tcgtccacct tcggattctt gtagaagctc gaggccttga acgtgcccca
     1081 ctggccggag tggaacatct cgccgatcca gttgttcgga tcggcgtagt aggtgctgat
     1141 ccagtagacg acgaggtccg gcgacgtctc cggcttcttc atccgctcga ccatcgcggg
     1201 ccacacctcg ctcgtgagct tggtgtccag cccgagcttg cgcaggccgt tggccatgac
     1261 ggtggccgct tgctcggtct ggctgaagcc ctggaggtag cccaccgtga gcggccgatc
     1321 gaccttggcc tgggcgagcc cgagctcctg cttcgccttg tccaggctgt aggtgtagcc
     1381 cttcacgtcg cgcggcacgc cccacatgtt gttcgggatg ggcaccgggt tgcgctccac
     1441 ggtgccgccg aggatctcct tgttgaagcc gtcgtagtcg aaggcgtagc tgatggcgcg
     1501 gcgaacgtgc acgtcggtca gcggtgcccg ctggttgttg atctggaaca tcatgatccg
     1561 catcgactcc gcctcgacga gcttgacgtt cgacgcctcg cgcagacgct tcacctggtc
     1621 gttgggcaga tagccgccgg tgccctggta gtcgcccttg atcatgccca gcacgcgggt
     1681 gttgtcttcc ttcacgaagc ggaagtcgat ctcctcgatg aacttcgcgc cccacggcat
     1741 gaagtggccc gggaagcgct tggcgacgaa gccgatcgcc gggtcgtagc gggtcagcat
     1801 gtaggagccg gagccggcct cgttgttggt cagccacgcc gcccccaggt ccccgtcctt
     1861 ctcgttcttc ttcaggaggg ccgagttgac cacgtgcacc tcgggcacca cggccaggaa
     1921 gatggccgcg ggcttggtga gcgtgaactg gacggtatag cgatccagcg ccttcgtggc
     1981 gccgggggcg atcatggtgg agatcagggc cgccgcgcct ttcttcatgg cgaggatgcg
     2041 ctcggcgctg tagcgcacgt cctctgcggt gatctcggcc ccgtcgtgga acttggcccc
     2101 gtggcgcagc ttgaaggtgt aggtgagccc gtccggggag acggtgtggc tctcggccag
     2161 ccacggctcg agcttcggcg ggttgtcgac ccagcggtag agcccgtcgt agaggttcag
     2221 gcgcaccgcc acccgcccga cgtcgaacgc cgcgtgcggg tccatcgtgt cgtagggact
     2281 ctcgttcgag aagacgaaac gcgtctgcgc ggtggccagc gccgggggga tcgccgcga
//
LOCUS       DATMCV010000116         2383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_140509, whole genome shotgun sequence.
ACCESSION   DATMCV010000116 DATMCV010000000
VERSION     DATMCV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140509"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VNU03_02835"
     CDS             complement(<1..309)
                     /locus_tag="VNU03_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73412.1"
                     /translation="MNASALPILLVEDSPDDALLIQRAFRKANLANPVQHVSDGEEAV
                     AYLSGAPPWSDRAKFPLPVFMLLDLKLPRRSGLEVLEWVRRESAVKRLPVVVLTSSRE
                     S"
     gene            complement(306..2093)
                     /locus_tag="VNU03_02840"
     CDS             complement(306..2093)
                     /locus_tag="VNU03_02840"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWP73413.1"
                     /translation="MPSTAAPTRLPIRARITLAFALALAVLVAIALVSYRSVGVLVET
                     SARVGHTHEVLGTLESLLGGVITAETGVRGYVFTGEERFLGTYWSGMQRAEIGLAALR
                     RLTADNREQQDRLAHLEPLLAERLLALQEISALRRERGRDVAAERVRLTGGSGLDASI
                     RGLIDEMRAAERLLLDARVASASAVASRTRWIIAGGSVGLVAITLGSILLIGREIERR
                     RRVDRGRQAAEARYQQLFDRNLAGMARTRRADGTVLECNPAMARILGYRSREEVLGLK
                     ARDLYVDLADREQIVSRVAPGGGLVDQEVRFRRKDGEVIWVSMTSVEVEDDGQPAFEA
                     LVLDVTDRHNAREQIQGLNTALARQVGELDAVNRELDAFSYSISHDLRAPLRAMQGFS
                     EALLEDYGDRLDATGHDYAQRIVAASRQMDTLIQDLLAYSRLVRTEIALDPVSLEAVV
                     DEACAPLEQEVKERGGEIAVERPLGRVLAHRTLLGQIVENLLTNALKFTRPEAPPRVR
                     IHAERGPGRLRLWVEDNGIGIAPEHRERIFRAFERLHGMQQYPGTGIGLAIVQKGALR
                     LGGQAGVESEPGAGSRFWVDLREAEAPAA"
BASE COUNT          336 a          899 c          820 g          328 t
ORIGIN      
        1 cgattctcgg gaggaggtga gcacgaccac cggcagacgc ttgacggcgg actcccggcg
       61 cacccactcc agcacctcga gcccggagcg ccgcggcagc ttgaggtcga gcagcatgaa
      121 caccggcagg gggaacttcg cccggtcgct ccacggcggc gccccgctca ggtaggcgac
      181 cgcctcttcc ccgtcgctca cgtgctgcac cgggttggcc aggttggcct tccggaaggc
      241 gcgctggatc aggagcgcgt cgtccgggga atcctccacg agcaggatgg gcagagcgga
      301 ggcgttcatg cggcgggcgc ctcggcctct cgcaggtcga cccagaagcg gctcccggcc
      361 ccgggctcgg actcgacgcc ggcctggccg ccgaggcgca gcgcaccctt ctgcacgatg
      421 gccaggccga tcccggtgcc cgggtactgc tgcatgccgt gcagccgctc gaacgcccgg
      481 aagatccgct cgcggtgctc gggggcgatg ccgatgccgt tgtcctccac ccagaggcgg
      541 agccggcccg gtccgcgctc ggcgtggatc cgcacgcgcg ggggcgcctc gggccgcgtg
      601 aacttgagcg cgttggtgag aaggttttcg acgatctggc cgagcaaggt gcggtgggcc
      661 agcacccgcc cgagcggccg ctcgaccgcg atctcgcccc cgcgctcctt gacctcttgc
      721 tcgagcggcg cgcacgcctc gtccaccacc gcctcgaggc tcaccggatc gagcgcgatc
      781 tcggtgcgga cgagacggct gtaggcgagc agatcctgga tcagggtgtc catctgccgg
      841 ctcgcggcca cgatccgctg cgcgtagtcg tggccggtgg cgtcgaggcg atcgccgtag
      901 tcctcgagca gggcctccga gaagccctgc atcgcccgca ggggcgcgcg caggtcgtgc
      961 gagatcgagt agctgaaggc gtcgagctcc cggttcaccg cgtcgagctc acccacctgc
     1021 cgcgcgagcg cggtgttcag cccctggatc tgctcgcgcg cgttgtgccg atcggtcacg
     1081 tcgagcacga gggcctcgaa ggccggctgg ccgtcgtcct ccacctccac cgaggtcatc
     1141 gacacccaga tcacctcgcc gtccttgcgt cggaagcgca cctcctggtc gaccagcccc
     1201 ccgcccggcg cgacgcggga gacgatctgc tcccgatccg ccaggtccac gtagagatcg
     1261 cgcgccttca ggccgagcac ctcctcgcgc gagcggtagc cgaggatccg cgccatcgcc
     1321 gggttgcact ccagcaccgt cccgtccgcc cgccgggtgc gcgccatccc ggcgaggttg
     1381 cggtcgaaga gctgctggta gcgcgcctcc gcggcctgtc gcccgcgatc gacccgccga
     1441 cgccgctcga tctcgcgccc gatgagcagg atggagccga gggtgatcgc cacgagcccc
     1501 acgctgcccc cggcgatgat ccaccgcgtg cgggaggcca ccgcggaggc gctggccacg
     1561 cgggcatcca gcagcaggcg ctcggccgcc cgcatctcgt cgatgagccc gcggatggac
     1621 gcgtccaacc ccgagccgcc ggtcagccgg acccgctcgg cggcgacgtc ccggccccgc
     1681 tcccggcgga gcgcgctgat ctcctggagc gccagcagcc gctccgcgag gagcggctcg
     1741 agatgggcga gccggtcctg ctgctcccgg ttgtcggcgg tgagccgccg gagcgcggcg
     1801 agcccgatct ccgcccgctg catccccgac cagtaggtcc cgaggaagcg ctcctcgccg
     1861 gtgaacacgt acccgcgcac cccggtctcc gcggtgatca cccctcccag cagcgactcg
     1921 agcgtgccga gcacctcgtg cgtgtggccc acccgcgcgc tcgtctcgac cagcaccccg
     1981 acgctgcgat agctgaccag cgcgatcgcc accagcaccg ccagcgcgag cgcgaatgcg
     2041 agggtgatcc gtgcgcggat cgggagccgg gtcggggctg cggtggacgg catcctgata
     2101 taaaagttag cacgcgggac ctttataccc gcgagacccg gaagctgacg cagtgcagca
     2161 cagaagacgg ccggatggcg ccgcctggag ggttttaact ccccttcact ctacctaccg
     2221 gcaacctacc ggccggcctg gcaccctacc ggcggccctg gaagcacctg gcgatctcga
     2281 actggagccg gttcgcctcc tcgcgcttgt ccggagggaa ccgcgcctcg gtggcctgcc
     2341 gcatggtcgt cgcggcaccc tgacagtcgc cgcgggagag cgc
//
LOCUS       DATMCV010000117         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_538812, whole genome shotgun sequence.
ACCESSION   DATMCV010000117 DATMCV010000000
VERSION     DATMCV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538812"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /gene="urtB"
                     /locus_tag="VNU03_02845"
     CDS             <1..1670
                     /gene="urtB"
                     /locus_tag="VNU03_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HWP73414.1"
                     /translation="APAVAQSAGASAAGAAPAAGPSAEIAKHIADVASADAEVQEAAA
                     VALGKTGDRKILPLLEALREGSVYVRPLPGGKKETVIVGDKLSEGDKTLVPLFRAYGR
                     EALTGADGKPLLADLTTLEEVSTGRRLRIAIRPLIDAFSGQSDLANPDPATRRAAATK
                     MGYTGDAATRQVLEEALGKERDRWARFALQEAIALIQLRTGSDAERVAAAEALGRLRS
                     ANAMEPLYQLAGDPQAPTPLAAAARSAIKRIERWGLFTQAIEIAFQGLSLSSILLLMA
                     LGLAIVFGLMGVINMAHGELMALGAYATFLMQGWFTAYFPAYFDYYFLVALPFSFLVS
                     AGAGFVLERGVIRFLYGRPLETLLLTWGVSLMIQQGLRLWFGAANVDVTSPRWLSGGI
                     PVMIGVQLPYNRLFIIGLATLCVGAMYLLLFRTDAGLRIRAVTQNRGMAACLGVRARW
                     VDAGTFALGAGLAGLAGCAITQIGNVGPELGQNYIVDSFMVVVTGGVGKLLGSILAAI
                     GIGGLNKIIEPGMGAVFGKVLILVLVIVFIQRRPAGLFATKGRYAES"
     gene            1657..>2083
                     /locus_tag="VNU03_02850"
     CDS             1657..>2083
                     /locus_tag="VNU03_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HWP73415.1"
                     /translation="MRSLEGWGLLGLALLLVVVLPALNALPAGAPLRVSDFTLNLFGK
                     FLAYAILALGIDLIWGYTGVLSLGHGVFFGLGAYAMGMHLMLEIGTRSVYGNVLPDFM
                     VWNRVTELPLFWKPFYSVPFTLLAIVVVPALFAFVFGYFT"
BASE COUNT          260 a          757 c          712 g          354 t
ORIGIN      
        1 ccgccccggc agtggcgcag agcgcggggg cttctgccgc cggcgcggct cccgccgccg
       61 gcccctccgc cgagatcgcc aagcacatcg ccgacgtggc ctccgccgac gcggaggtcc
      121 aggaagcggc cgcggtggcg ctcggcaaga ccggcgaccg caagatcctg ccgctgctcg
      181 aagccctccg cgaggggagt gtctacgtcc ggcctctgcc gggcggcaag aaggagaccg
      241 tcatcgtggg ggacaagctg agcgaggggg acaagaccct cgtgcccctc tttcgcgcct
      301 acgggcgcga ggctctcacc ggcgccgacg gcaagccgct gctcgcggac ctgaccacgc
      361 tcgaggaggt gtcgaccggc cggcgcctcc gcatcgcgat ccgcccgctg atcgacgcgt
      421 tctccggcca gagcgatctc gccaatcccg atccggccac ccggcgcgcc gccgccacca
      481 agatgggcta caccggcgac gcggcgaccc ggcaggtgct cgaggaggcg ctcggcaagg
      541 agcgggaccg gtgggcccgc ttcgcgctgc aggaggcgat cgcgctgatc cagctgcgca
      601 ccggcagcga cgcggaacgg gtggccgccg ccgaggcgct cgggcgcctc cgcagcgcca
      661 acgccatgga gccgctctac cagctcgcgg gcgacccgca ggccccgacc ccgctcgccg
      721 cggccgcccg cagcgcgatc aagcgcatcg agcgctgggg cctcttcacc caggccatcg
      781 agatcgcctt ccagggcctc tcgctctcgt ccatcctgct cctgatggcc ttgggtctcg
      841 ccatcgtgtt cggcctcatg ggcgtgatca acatggccca cggcgagctg atggcgctcg
      901 gcgcctacgc cacgttcctg atgcaggggt ggttcaccgc gtacttcccg gcctacttcg
      961 actactactt cctggtcgcg ctgccgttct cgttcctcgt gtcggccggc gccgggttcg
     1021 tgctggagcg gggcgtgatc cgcttcctct acggccggcc gctcgagacc ctgctgctga
     1081 cctggggcgt gagcctgatg atccagcagg gcctgcggct gtggttcggc gccgccaacg
     1141 tcgacgtgac atccccccga tggctctcgg gtggcatccc ggtgatgatc ggagtccagc
     1201 tcccgtacaa ccggctcttc atcatcggcc tcgccacgct gtgcgtgggc gcgatgtacc
     1261 tcctcctgtt ccgcaccgac gcgggcctgc ggatccgcgc ggtgacccag aaccggggga
     1321 tggcggcctg cctgggcgtg cgggcgcgct gggtcgacgc gggcaccttc gcgctcggcg
     1381 cggggctggc cgggctggcc ggctgcgcga tcacccagat cggcaacgtg ggccccgagc
     1441 tcgggcagaa ctacatcgtg gactcgttca tggtggtggt gaccggcggg gtcggcaagc
     1501 tcctcggcag catcctcgcc gcgatcggga tcggcggcct gaacaagatc atcgagcccg
     1561 gcatgggcgc cgtcttcggg aaggtgctga tcctggtcct cgtgatcgtg ttcatccagc
     1621 gccggcccgc cggtctcttc gcgaccaagg gacgctatgc ggagtcttga gggctggggc
     1681 ctcctcggcc tggcgctgct gctggtggtg gtgctgcccg cgctcaatgc gctgcctgcg
     1741 ggcgcgccgc tgcgggtgtc ggacttcacc ctgaatctct tcggcaagtt cctggcctac
     1801 gccatcctgg ccctcggcat cgacctgatc tggggctaca ccggggtcct ctccctaggc
     1861 cacggggtct tcttcggtct cggggcctac gcgatgggca tgcacctgat gctcgagatc
     1921 ggcacccgca gcgtctacgg caacgtgctc cctgacttca tggtgtggaa ccgggtgacc
     1981 gagctgcccc tcttctggaa gccgttctac agcgtgccct tcaccctgct cgcgatcgtg
     2041 gtggtgcccg cgctcttcgc gttcgtgttc ggctacttca cgt
//
LOCUS       DATMCV010000118         6821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_872791, whole genome shotgun sequence.
ACCESSION   DATMCV010000118 DATMCV010000000
VERSION     DATMCV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872791"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VNU03_02855"
     CDS             complement(<1..533)
                     /locus_tag="VNU03_02855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP73416.1"
                     /translation="MASVTGRTVLITGAARGIGAACARRLAGDGAKLVLVDVDGPGVE
                     KLAAELGQVAVRADVTKPDDIARLVDEPYKRWGRLDVLFNNAGVIRVQPMLDVTEAEW
                     DRVMDVNLRAVFFVLQAAVRRMLKQDLIPGSELRGKLIQTASIAAYRGGNHLMTPYSA
                     TKAGVVSVTRSAAQALAG"
     gene            complement(521..1483)
                     /locus_tag="VNU03_02860"
     CDS             complement(521..1483)
                     /locus_tag="VNU03_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73417.1"
                     /translation="MRSAEDAGLLEVVVADGRPLLLCTALALLFSGAFAIFLSVRREF
                     LPHDVAFLGMTPDQLCALGDCRVVAFMFHDRVAFGGVLIAIAVLYLWLTTVPLAAGAA
                     WAWWTILVSGTVGFASFLAYLGYGYLDGWHGVGTAFLAPLFAAGLVRSRALVRDGPVG
                     PVHAVFHALDRRRRLGQAALAATGLGMLLAGATILIVGMTRVFVPQDLAFMGLTVGDL
                     HAANPRLVALIAHDRAGFGGGLFCCGTIVIACALFARPSRSLRQALALAGAAGFGAAI
                     GVHLAVGYTDPLHLAPAVAGAALFVIGLVLVSERQPRGETRWHR"
     gene            complement(1480..2169)
                     /locus_tag="VNU03_02865"
     CDS             complement(1480..2169)
                     /locus_tag="VNU03_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73418.1"
                     /translation="MLALAVLSGWVRPGAAEPTLPLPPEVPGKDRHRLEEIAKRAFAS
                     TQVTFEAYPVRPEIFEYLLDHPEFASHVTRALRAARYKIWRESDSLWLDDGWGVRGTF
                     AVVYAEPGLRLMHAQGAYQSSLPDIKGRAVVALAYKFQPAAPGRPQVLTTLTSFVQID
                     SAVVRTIGRMGGPLVQKKADREARQLLKVFSRVSRAIEDNPADVYARVRERPDVPKAE
                     LEEFRKLLRVP"
     gene            complement(2219..3988)
                     /locus_tag="VNU03_02870"
     CDS             complement(2219..3988)
                     /locus_tag="VNU03_02870"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWP73419.1"
                     /translation="MTLDSAQYQRSAAPAGSGPMTQVMGRKWMVAAGHPLAAQAAARV
                     LAAGGNAIDAGVAAGFMLGVVHPDMVSFAGVAPILVHLASTGETYEVSGVGPYPRRAS
                     AEYFRTKHGGDIPIGLLRTVVPSAPDSWCVALERWGTLSFADTVAGALECADHGFPTS
                     AFSASIMASNAERYKRFPTSAAIFLPGGKPHAAGQLFVQSELAATIKEMVTAEVRARS
                     RGRAGAIRAARDAFYKGDIAQRIADYHAREGGLLTREDLAEYQAEVAPALKTTFHDYE
                     VAACGFWCQGPVFLQMLNLIEAYDWPALGHNSPRGLHLMVEAMKLAFADREAYYGDPR
                     FVKVPAEALLSKAYAELRRGLIREDRAWPDMPPPGDPEGKAAVRNGHAAGSKIGAPSH
                     AGTLDTSYVSVIDAEGNAFSATPSDPGGVDSPVIPGVGCVVSPRGSQGWLIPGHASEV
                     AGGKRPRLTPAPSLVLRDGRAFMAFGTPGGDVQQQAMLQVLLNITAFGMAPQQAVEAP
                     RLATRSFPDSFWPHANSPGRLDVEARIPHETRAALASLGHVITDWPDYDWRAGAVCAS
                     MVGKDGVRLGGADPRRGAHAIGW"
     gene            4012..4737
                     /locus_tag="VNU03_02875"
     CDS             4012..4737
                     /locus_tag="VNU03_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWP73420.1"
                     /translation="MSRRAPLIGVSTSITVGTHPERAYVNSTYLHAVQQAGGVPVALP
                     PQLSTASMRQLAAELDGLLLTGGGDLDPALFDETPHATLYDVAPARDTLETAALHVAL
                     DRGRPVLAVCRGLQVLNVALGGSLYQDVGTDPGTQILHSQTEPRDQLTHKVKVTPGSR
                     LAETLGTDELETNSMHHQAIKRLGRGLTAVAWAPDQIVEGAELGDPARFVLGVQWHPE
                     ELVGHSEPARRLFAALVAAARKS"
     gene            4866..6227
                     /locus_tag="VNU03_02880"
     CDS             4866..6227
                     /locus_tag="VNU03_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HWP73421.1"
                     /translation="MVTAIYALLTALLVAFFYVSWGSPLGNLEHAEESLERLISREMD
                     LRQSYEHAPRWERRLYELTGSDPESFDDAIGRFDEFDDDQRSPRTDLDLVVLLGEAGL
                     LERAAGIIETLGGTEGESAQYSHWLEAAYLDAPSSEEATALSEEIRRELPGDWFTDTL
                     VRRIASRVDDQELQARAESAIEARGRSMLTRVRALTGTGAALLVLAAVVALRLRSFRR
                     DPRVADAPLPPHWWGADGTGLFFRAAFAYLLIPAAVVLLVPRVPPVTAIMGLVGGLPT
                     LWWARRYLHARGLTVRATFGLSPRPGRLPRLLGWGLVLLAVSMVGESLLYMGLGALGV
                     SSHWADGFLEEFLWGPPTAVVAGTLDGVVWAPIFEELAFRGLLYPTLRLRLPAWLAAL
                     LSAAIFAFAHGYGVQGFAAVTWSGMVWALGYERTRSLLPGMLAHAGSNLLATSSFLLL
                     LRF"
     gene            complement(6235..>6821)
                     /locus_tag="VNU03_02885"
     CDS             complement(6235..>6821)
                     /locus_tag="VNU03_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWP73422.1"
                     /translation="TGVERTFYVPGENDVFADGGTEYLNRFAVGGPGRGWQTFDYRGV
                     HFVGLVNVLNFKAGKLGSLGPDQLEWLERDLAPLASSTPVVVFAHVPLWAVYPEWGWV
                     TDDGAQALGYLKRFGSVTVLNGHIHQIMQKVEGNVTFHTARSTAFPQPAPGVGSGPGP
                     IKVPVEELRGVLGVREVRYTAGRDRLAVVDTSLA"
BASE COUNT          934 a         2539 c         2455 g          893 t
ORIGIN      
        1 ccggcgaggg cctgcgcggc ggagcgggtg acgctcacca ccccggcctt ggtcgccgag
       61 tacggcgtca tcaggtggtt ccctccgcgg taggcggcga tggaggcggt ctggatcagc
      121 ttcccgcgga gctccgaccc cgggatcagg tcctgcttca gcatccgccg caccgccgcc
      181 tggaggacga agaagaccgc gcgcaggttc acgtccatca cccggtccca ctccgcctcg
      241 gtcacgtcga gcatcggctg cacccggatg acccccgcgt tgttgaacaa cacgtcgagg
      301 cgcccccagc gcttgtaggg ctcatccacc aggcgcgcga tgtcgtccgg cttcgtcacg
      361 tcggcgcgca ccgccacctg gcccagctcg gccgcgagct tctcgacccc gggcccgtcg
      421 acgtccacca gcaccagctt cgcgccgtct cccgccagtc gccgcgcgca ggccgcgccg
      481 atgccgcgcg ccgcgccggt gatcagtacc gtgcgcccag tcaccgatgc catcgggtct
      541 cccctcgtgg ttggcgttcg ctcaccagca cgagacctat cacgaacagg gccgctcccg
      601 ccaccgcggg cgccaggtgc agcggatcgg tgtagccgac cgcgaggtgg acgccgatgg
      661 cggccccgaa tccggccgcg ccggcgaggg cgagcgcctg ccggaggctg cgcgacgggc
      721 gcgcgaacag ggcgcacgcg atcacgatgg tgccgcagca gaagagcccg ccgccgaagc
      781 cggcgcgatc gtgcgcgatg agggcgacga gccggggatt ggccgcgtgg aggtccccga
      841 cggtcaggcc catgaaggcc aggtcctggg ggacgaagac gcgggtcatc ccgacgatca
      901 ggatcgtggc gccggcgagg agcatcccga gcccggtggc cgcgagcgcc gcctgtccca
      961 ggcgccggcg gcggtccagg gcgtggaaga cggcgtgcac cgggccgacc gggccgtccc
     1021 gcacgagggc gcgggaccgc acgagtccgg ccgcgaacag cggggccagg aaggcggtcc
     1081 cgaccccgtg ccagccgtcc aggtagccgt agccgagata ggcgagaaag ctcgcgaagc
     1141 cgacggtgcc gctgaccagg atcgtccacc acgcccacgc ggctcccgcc gcgagcggca
     1201 ccgtggtcag ccacaggtag agcaccgcga tcgcgatcag gaccccgccg aaggctaccc
     1261 gatcgtggaa catgaacgcc accacccggc agtcgccgag cgcgcagagc tggtcgggcg
     1321 tcatgccgag gaacgccacg tcgtggggca ggaactcgcg gcggacggag aggaagatcg
     1381 cgaaggcgcc ggagaacagc agggcgagcg cggtgcacag gaggagcggc cggccgtcgg
     1441 ccacgaccac ctcgagcagc cccgcgtcct ccgcggatct cacggcacgc gcagcagctt
     1501 ccggaactcc tccagctccg ccttcggcac gtcggggcgc tcgcggacgc gcgcgtagac
     1561 gtcggccggg ttgtcctcga tggcccggct cacccgggag aacaccttca ggagctgccg
     1621 cgcctcgcga tcggccttct tctgcacgag gggcccgccc atgcggccga tggtgcggac
     1681 caccgcgctg tcgatctgca cgaacgaggt gagcgtggtg agcacctgcg ggcggcccgg
     1741 cgcggcgggc tggaacttgt aggcgagcgc caccaccgcc cggcccttga tgtcggggag
     1801 cgaggactgg taggccccct gggcgtgcat caggcgcagc cccggctcgg cgtacaccac
     1861 cgcgaaggtg ccgcggacgc cccagccgtc gtcgagccag agcgagtcgc tctcgcgcca
     1921 gatcttgtag cgggcggcgc ggagcgcgcg ggtcacatgg ctcgcgaact cggggtgatc
     1981 cagcaggtac tcgaagatct cgggacggac cggatacgct tcgaaggtca cctgggtcga
     2041 ggcgaaggcg cgcttggcga tctcctcgag gcgatggcgg tccttgcccg gcacctcggg
     2101 aggcaacggg agcgtgggct cggccgcgcc cgggcggacc cagcccgaca gcacggcgag
     2161 cgccagcaag gctacgagcg cgagatgccg ggaccgcacc tggcgcggag tcgcgccgct
     2221 accacccgat ggcgtgggcg ccgcggcgcg ggtcggcccc accgagccgc accccgtcct
     2281 tgccgaccat cgaggcgcag accgcacccg cccgccagtc gtagtcgggc cagtcggtga
     2341 tgacgtgccc gaggctcgcc agcgccgcgc gcgtctcgtg ggggatgcgc gcctcgacgt
     2401 ccaggcgccc gggggagttg gcgtggggcc agaaggaatc ggggaaggag cgcgtggcca
     2461 gccgcggcgc ctccaccgcc tgctgcgggg ccatgccgaa ggcggtgatg ttgagcagca
     2521 cctgcagcat cgcctgctgc tggacatcgc cgcccggcgt gccgaaggcc atgaaggcgc
     2581 ggccgtcgcg cagcaccagc gagggcgcgg gggtgaggcg cggccgcttg ccgcccgcca
     2641 cctcgctcgc gtggccgggg atgagccagc cctgggagcc ccgcggcgag accacgcagc
     2701 ccacgccggg gatgaccggc gagtccacgc cgccggggtc gctcggggtg gccgagaacg
     2761 cgttgccctc cgcgtcgatc accgagacgt aggacgtgtc gagggtgccc gcgtgcgacg
     2821 gcgcgccgat cttcgatccg gcggcgtggc cgttgcgcac cgcggccttg ccctccggat
     2881 cgcccggcgg cggcatgtcg ggccacgcgc gatcctcgcg gatcaggccg cggcgcagct
     2941 cggcgtaggc cttcgacagc agcgcctccg cgggcacctt cacgaagcgg gggtcgccgt
     3001 agtaggcctc acggtcggcg aaggcgagct tcatcgcctc caccatgagg tgcaggccgc
     3061 gcgggctgtt gtgacccagc gcgggccagt cgtacgcctc gatcaggttc agcatctgga
     3121 ggaagaccgg cccctggcac cagaagccgc acgcggccac ctcgtaatcg tggaaggtgg
     3181 tcttgagcgc gggcgcgact tccgcctggt actcggcgag atcttcccgg gtgagcaggc
     3241 cgccctcgcg cgcgtggtag tccgcgatgc gctgggcgat gtcgcccttg tagaacgcgt
     3301 cgcgggcggc ccggatggcc cccgcgcggc cgcggctgcg ggcgcgcacc tcggcggtca
     3361 ccatctcctt gatcgtcgcg gccagctcgg actgcacgaa cagctggccc gccgcgtggg
     3421 gcttgccgcc gggcaggaag atcgcggcgg aggtggggaa gcgcttgtag cgctccgcgt
     3481 tgctggccat gatcgaggcc gagaacgccg aggtcgggaa gccgtggtcc gcgcactcga
     3541 gcgcgccggc caccgtgtcc gcgaacgaga gcgtgcccca gcgctcgagc gccacgcacc
     3601 aggagtcggg cgcggacggc acgacggtgc gcagcaggcc gatcgggatg tcgcccccgt
     3661 gcttggtgcg gaagtactcg gcgctggcgc ggcgcgggta cgggccgacc cccgagacct
     3721 cgtaggtctc gccggtcgag gccaggtgga ccaggatcgg ggcgacgccc gcgaagctca
     3781 ccatgtcggg atggacgacc cccagcatga agcccgcggc cacgcccgcg tcgatggcat
     3841 tgccacccgc ggcgagcacg cgcgcggccg cctgcgccgc gagcggatgt cccgccgcca
     3901 ccatccactt ccgtcccatc acctgcgtca tcgggcccga gcccgccggc gcagcgctgc
     3961 gctggtattg cgcggagtcg agggtcatgg gcggtatggt agcatggcgc catgagtcgc
     4021 cgcgcgcccc tcatcggcgt gagcacgtcc atcacggtgg gcacgcatcc ggagcgcgcg
     4081 tacgtcaact cgacgtacct gcacgcggtg cagcaggccg gaggcgtccc ggtcgcgctg
     4141 ccgccccagc tctcgaccgc gtccatgcgc cagctggccg ccgagctcga cgggctcctg
     4201 ctgaccgggg gcggcgacct cgacccggct ctcttcgacg agaccccgca cgccaccctc
     4261 tacgacgtgg cgcccgcgcg cgacacgctc gagaccgcgg ccctccacgt ggcgctcgat
     4321 cgcggccggc ccgtcctcgc ggtgtgccgc ggcctccagg tgctgaacgt ggccctgggg
     4381 ggcagcctct accaggacgt gggcaccgac cccggcaccc agatcctgca cagccagacc
     4441 gagccccggg atcagctcac ccacaaggtg aaggtcacgc cgggcagccg gctcgccgag
     4501 acgctcggca ccgacgagct cgagaccaac agcatgcacc accaggcgat caagcggctc
     4561 ggccgggggc tcaccgcggt ggcgtgggcg ccggaccaga tcgtcgaggg cgcggagctc
     4621 ggcgaccccg cgcgcttcgt gctcggcgtg cagtggcatc cggaagagct cgtgggccac
     4681 tcggagccgg cccggcgtct cttcgccgcc ctcgtggccg cggcccgcaa atcctgaccc
     4741 cgcccgccga agggcccgct gccgaagggc ccgccgaggc gccgtctccc ggcgcgcccg
     4801 cggtattccc cgacgctccg ccgatcgtcc cggagtcccc ggccccgacc cgctacttct
     4861 ccccgatggt caccgcgatc tacgcgctgc tcaccgcgct gctggtcgcg ttcttctacg
     4921 tcagctgggg ctccccgctc ggcaacctgg agcacgccga ggagtcgctc gagcgtctga
     4981 tctcgcggga gatggatctt cgccagtcct acgagcacgc cccccgctgg gagcgccgac
     5041 tctacgagct gaccggatcg gatcccgaga gcttcgatga cgcgatcgga cgcttcgacg
     5101 agttcgacga cgaccagcgg tctccccgca ccgacctgga cctggtggtg ctgctgggcg
     5161 aggccgggct gctcgagcgg gccgccggca tcatcgagac cctcggcggc accgaggggg
     5221 aaagcgccca gtacagccac tggctcgaag ccgcctatct cgacgcgccc tcctcggagg
     5281 aggccaccgc gctctccgag gagatccgcc gcgagctacc cggtgactgg ttcaccgaca
     5341 ccctggtgcg ccggatcgcc agccgggtgg acgaccagga gctgcaggcc cgcgccgagt
     5401 ccgccatcga ggcgcgcggc cgctcgatgc tgacgcgggt gcgcgcgctc accggcaccg
     5461 gcgccgcgct gctggtgctc gccgccgtgg tggcgctgcg cctgcggtcc ttccggcggg
     5521 acccgcgggt ggccgacgcg ccgctgcctc cgcactggtg gggcgccgac gggaccggcc
     5581 tcttcttccg ggccgcgttc gcctacctgc tgatcccggc cgcggtggtg ctcctggtgc
     5641 cccgcgtgcc cccggtcacc gcgatcatgg ggctggtggg cggcctgccg acgctctggt
     5701 gggcgcggcg ctatctgcac gcgcggggcc tgacggtgcg ggccacgttc ggcctgagcc
     5761 cgcggcccgg ccggctgccg cgcctcctcg gctggggcct cgtgctcctc gcggtctcga
     5821 tggtgggcga gagcctgctc tacatgggcc tgggtgcgct cggcgtcagc tcgcactggg
     5881 cggacgggtt cctcgaggaa ttcctctggg gccccccgac cgcggtcgtg gcgggcaccc
     5941 tcgacggcgt ggtgtgggcg ccgatcttcg aggagctggc cttccgcggc ctgctctatc
     6001 ccaccctgcg cctccgcctc cccgcctggc tcgccgcgct gctcagcgcc gccatcttcg
     6061 cgttcgcgca cggctacggc gtccagggct tcgccgcggt cacctggagc ggcatggtgt
     6121 gggcgctcgg ctacgagcgg acgcgcagcc tgctgcccgg catgctggcc catgccggca
     6181 gcaacctgct cgccaccagc agcttcctcc tgctgctgcg cttctagacg cggctcacgc
     6241 gagcgaggtg tcgaccaccg ccaggcggtc gcggccggcc gtgtagcgga cctctcgcac
     6301 gcccagcacg ccacgcagct cctccaccgg caccttgatc ggacccggcc cggatcccac
     6361 gccgggggcg ggctgcggga aggcggtgga ccgcgcggtg tggaacgtca cgttgccctc
     6421 caccttctgc atgatctggt ggatgtgacc gttgagcacg gtcaccgagc cgaaccgctt
     6481 gagatatccg agcgcctgcg ctccgtcatc ggtgacccag ccccactccg gatagaccgc
     6541 ccagagcggc acgtgcgcga agaccacgac gggggtgctg ctcgcgagcg gcgccaggtc
     6601 ccgctcgagc cactcgagct gatccgggcc caggctgccg agcttgcccg ccttgaagtt
     6661 gagcacgttg acgagcccca cgaagtgcac cccgcggtag tcgaacgtct gccagccgcg
     6721 gcccggcccg cccaccgcga accggttcag gtactcggtg ccgccgtccg cgaacacgtc
     6781 gttctctccc ggaacgtaga aggtccgctc gacgccggtg c
//
LOCUS       DATMCV010000119         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_938745, whole genome shotgun sequence.
ACCESSION   DATMCV010000119 DATMCV010000000
VERSION     DATMCV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938745"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..573
                     /locus_tag="VNU03_02890"
     CDS             112..573
                     /locus_tag="VNU03_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP73423.1"
                     /translation="MKYWEDFKVGESTDLGSHSMTEAEILAFARKYDPQPFHTDPEAA
                     RRSIFGGLIASGWHTCALMMRLSVEANRREEAAATGSPGLDSCRWLKPVRPGDTLTGR
                     TEVLETWPSKSKPIGFVRRRVEMVNQQGEAVVAIVGITMYRRRPTGAAATA"
     gene            570..1004
                     /locus_tag="VNU03_02895"
     CDS             570..1004
                     /locus_tag="VNU03_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP73424.1"
                     /translation="MKYFEDVAVGDIMRFGRYEVTKEEIVEYARQFDPQPFHLDEEAA
                     RQSMFGGLIASGWHTGAMFIRMVVEHMTPVHATTGAMGFDDLKWVKPVRPGDVLTVES
                     EVKEKVEGRRPDRGTVKIESRIQNQRGEVVMSLVSLVIYLRR"
     gene            1009..1566
                     /gene="nbaC"
                     /locus_tag="VNU03_02900"
     CDS             1009..1566
                     /gene="nbaC"
                     /locus_tag="VNU03_02900"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HWP73425.1"
                     /translation="MRGLVAFNLWRWIEENGPSFEPPVGNKVIWEDSQFTAMVVRGPN
                     ARRDFHVDPSDEIFYMLRGAMRLEYMEDGRRHKRIIREGEMCLLPALVPHSPHRPADT
                     WGLVIEVKRRPEQTESLLWFCERCDRLLHQVTMHVADIEGELKAAIEGFDASLALRTY
                     GGCGHVHAESAPPVAPVTPEDRSRG"
     gene            complement(1538..>2817)
                     /locus_tag="VNU03_02905"
     CDS             complement(1538..>2817)
                     /locus_tag="VNU03_02905"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HWP73426.1"
                     /translation="PFHAVIAGDVAVLLAIAMLLAGNPGWERLTAPFMLWAPVATPLL
                     VAGEAIRRAFELVGLVATPRYRPLLRGGLFALPVVGLFTLILANADPVLATWRDDLAA
                     ALARLEFVPRLMFFGVLFTMALGGCGIVLNPGSARSLAPAEPARTPRLADTERLVILG
                     AVGALFGAFLLLQLTYLFGNAPNVRGSGVTFSEYARQGFAELTTVATLCTILLVALDH
                     WAARGPRERWARIAAVVVVGQVEVLLISALRRLWLYESAYGFTTVRLYAHAYMVVVAL
                     FLGMLGWRLARGLTSGWLARGAAGIAAVAMAVLIYWNHEAWIVRQNVGRFLRTHQLDT
                     SYLVWGLSPNAAPALVRAIPELPPLLADTVRDGLWQRYGKAPHGAPCRWFEWNRGRER
                     AAAALRADRIVRGDAQLHPHDCVLISPASGPPA"
BASE COUNT          469 a          944 c         1037 g          367 t
ORIGIN      
        1 cacggtgctc gagtccgagc tccggtggat ctggctgccc gcgcttgccc tcggctttat
       61 cctgatcggg gcgcgtcgcg cctggtcgcg gccccaccac ggaggtcacg cgtgaagtac
      121 tgggaagatt tcaaggtcgg cgagagcacc gatctcggct cgcactcgat gaccgaggcc
      181 gagatcctgg ccttcgcccg caagtacgat ccgcagccgt tccacaccga ccccgaggcg
      241 gcgcgccgga gcatcttcgg cggcctgatc gcgagcggct ggcacacgtg cgccctgatg
      301 atgcgcctgt cggtggaggc caaccgccgg gaggaagcgg ccgccaccgg ctcgcccggg
      361 ctcgactcct gccgctggct caagccggtg cggccgggcg acacgctgac cgggcgtacc
      421 gaggtgctcg agacgtggcc gtcgaaatcg aagccgatcg ggttcgtccg gcgccgcgtc
      481 gagatggtga accagcaagg cgaggcggtg gtggcgatcg tcggcatcac gatgtaccgg
      541 cggcggccca ccggcgcggc ggcgacggca tgaagtactt cgaggacgtc gcggtgggcg
      601 acatcatgcg cttcgggcgc tacgaggtca cgaaggagga gatcgtcgag tacgcgcgcc
      661 agttcgatcc gcagccgttc cacctcgacg aggaggccgc gcgccagagc atgttcggcg
      721 gcctcatcgc gagcggctgg cacaccgggg ccatgttcat ccggatggtg gtcgagcaca
      781 tgacgcccgt gcacgccacc accggggcga tgggcttcga cgacctcaag tgggtcaagc
      841 cggtgcggcc cggcgacgtg ctgaccgtgg agtcggaggt gaaggagaag gtcgagggcc
      901 gccggccgga tcgcgggacg gtgaagatcg agagccggat ccagaaccag cgcggcgagg
      961 tggtgatgag cctcgtgtcg ctggtgatat atctgaggcg gtgacccggt gcgtggcctc
     1021 gtcgccttca acctgtggcg gtggatcgag gagaacgggc ccagcttcga gccgccggtc
     1081 ggcaacaagg tgatctggga ggactcgcag ttcaccgcga tggtcgtccg cgggccgaac
     1141 gcccgtcgcg acttccacgt ggacccctcc gacgagatct tctacatgct ccggggcgcg
     1201 atgcgcctcg agtacatgga ggatggccgg cggcacaagc ggatcatccg cgagggcgag
     1261 atgtgcctgc tgcccgccct ggttccgcat tcgccccatc gccccgcgga cacgtggggg
     1321 ctggtgatcg aggtcaagcg ccggcccgag cagaccgagt cgctcctctg gttctgcgag
     1381 cgctgcgatc ggctcctcca ccaggtgacg atgcacgtgg ccgacatcga gggggagctc
     1441 aaggcggcca tcgagggctt cgacgcgagc ctcgcgctgc gcacatacgg cggctgcggg
     1501 cacgtgcacg cggagagcgc gccccccgtc gccccggtca cgccggagga ccgctcgcgg
     1561 ggctgatcag cacgcagtcg tgtgggtgga gctgggcgtc gcctcgcacg atgcggtccg
     1621 cgcggagcgc cgccgccgcg cgttcccggc ctcgattcca ctcgaaccag cgacagggag
     1681 cgccgtgagg ggccttcccg tagcgctgcc agagtccgtc gcgcaccgtg tcggccagga
     1741 ggggcggtag ctcgggaatc gcgcggacca gagccggcgc ggcgttcggc gagaggcccc
     1801 agaccaggta ggacgtgtcg agctgatggg ttcgaaggaa gcgcccgacg ttctgtcgga
     1861 cgatccacgc ttcgtggttc cagtagatca ggaccgccat cgcgacggcg gcgatccccg
     1921 ccgcccctcg cgccagccac cccgacgtca agccccgggc cagcctccag ccgagcattc
     1981 cgaggaagag cgcgaccacg accatgtagg cgtgggcgta gagccgcacg gtcgtgaacc
     2041 cgtaggcaga ctcgtagagc cagaggcggc ggagcgccga gatcaggagc acctcgacct
     2101 gcccgacgac cacgaccgcc gcgatgcgcg cccaccgctc gcgcgggccg cgcgcggccc
     2161 agtggtccag ggcgaccagc aggatcgtgc aaagggtcgc caccgtggtc agctcggcga
     2221 agccctggcg agcgtattcc gagaaggtga cgcccgatcc ccggacgttc ggggcgttgc
     2281 cgaagaggta ggtgagctgc aggaggagga acgcgccgaa gagcgcgccg accgcgccga
     2341 ggatcacgag gcgctcggtg tccgcgaggc gaggtgtccg agccggctcc gccggcgcca
     2401 ggctccgggc gctgccggga ttcaggacga tgccgcaccc acccagggcc atggtgaaca
     2461 ggacgccgaa gaacatgagc cggggcacga actccaggcg ggccagcgcc gcggccagat
     2521 catcacgcca cgttgcgagc accggatcgg cgttcgcgag gatcagcgtg aagagaccca
     2581 ccaccgggag cgcgaagagc ccgccgcgaa ggagaggccg gtagcgaggc gtcgcgacga
     2641 gaccgacgag ctcgaacgcg cgccggatcg cctctccggc caccaggagc ggcgtggcca
     2701 cgggcgccca tagcatgaac ggtgcggtca gcctctccca ccccgggttg ccggccagga
     2761 gcatggcgat cgccaggagc acggccacgt caccggcgat gacggcatga aagggcg
//
LOCUS       DATMCV010000120         6446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_272794, whole genome shotgun sequence.
ACCESSION   DATMCV010000120 DATMCV010000000
VERSION     DATMCV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272794"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VNU03_02910"
     CDS             <1..1380
                     /locus_tag="VNU03_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWP73427.1"
                     /translation="PMLLLPLGHPRDRAQVFPLFSSARTFASVTKSVETITVGGQVAD
                     VMRRALSLMRMGRPGPVMVEIPADLANEEVPEALIDAYRPVKATTAGANARDVETAAR
                     VLREARRPVIHAGQGVLYAEASADLVELAELLQAPVMTTLEGKSAFPEDHPLALGTGG
                     PVVTGHTLQYLREADVVFGIGCSFTRHGMAATIPVGKTLIHATNDERDLNKSYAADYP
                     LLGDARLVLRQFIEAIKSQGGRPAGREQVRADLDRVRAAWLAEWSATLGSTEVPMTPY
                     RVIAEFMRAVDPREAIVTHDSGSPRDQLLPFYRAVPPRSYLGWGKSHALGTGLGLIIG
                     AKLAAPEKFCVNFMGDAAFGMTGLDFETAVRCGVPILTVVLNNSAMAIEIPHLVVSHD
                     KYGTRDIGGRYADMGRAMGGWSERVERPEDIAGAFARARKATEGGQAALLEFITSQET
                     RFSNRGVLR"
     gene            1391..2302
                     /locus_tag="VNU03_02915"
     CDS             1391..2302
                     /locus_tag="VNU03_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWP73428.1"
                     /translation="MTRIAYFNGKFVPETEVLIPYRDRSFNRGDGCFDMTRTFDGRIF
                     RLEEHVERLYRSLRYLRIDIGLEPKEIVAISEEVVLRNEPLRAAAGDWWLAQRVSRGV
                     DAIGDEGWAHTGPQVIVDCTPLPLKARAAQFRDGIDVVVPSIRRTPPSTLSPRAKTHN
                     YLNLILADKEVKAAQPQAWSVLLDENGNLCEGVGSNVFVVRDERIYTPREKYVLPGVS
                     RRTAMELGTKLGLAVEERDLDVYDAVTADEVFLTSTSLCVCPVRSVNGHVIGDGRVPG
                     PVTRRITQAYIEHVGTDFVAQYLSRLG"
     gene            2319..3380
                     /locus_tag="VNU03_02920"
     CDS             2319..3380
                     /locus_tag="VNU03_02920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWP73429.1"
                     /translation="MTSSRRVQVRALATLLLVILLAGPAFAQAPAYPGATWTRTSSPE
                     QLGWSSEKLKEAKAYAATINTAALMVVVNGTVLDEWGETAKRFNVHSIRKSFLSALYG
                     IQVREGRIHLSTNLADLGIDDRGSLTGVEKGATVADLLKARSGIYHPALYETPEMAAG
                     RPARGSYSPGTFWYYNNWDFNALGTIYEQAVKASIFEEFKSRIADPLEMEDFRIADGS
                     YVRGPESIHAAYPFRMTARDMARFGLLYLRGGQWRGRSVVPREWVEESTRAFSDLGQS
                     GGYGYLWWVAPNGGPHFTSSYFNGRVFSARGAGGHFIVVVPYLDLVVVHRVDTDVQGR
                     QVSGAQFGHLMQLILDARR"
     gene            3515..4759
                     /locus_tag="VNU03_02925"
     CDS             3515..4759
                     /locus_tag="VNU03_02925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP73430.1"
                     /translation="MPRLNFGAFIAPHHPIGEHPMLQFRRDLDLVEHLDKLGYEEFWC
                     GEHHSSGWEMIASPEMFLAAAAERSKRIKLATGVISLPYHHPFNVAQRMVQLDYMTGG
                     RAIFGSGPGALASDAHTLAIDPMLLRDRQDEAIGVIRRLFRGERVSYKSDWFTLQDAA
                     LQLLPLQEEMPCAVASQISPSGMTLAGKHGIGIISIGSLSEEGLNALPTQWSFAEDAA
                     AKHGQTVDRKNWRVLLSWHIAETREKARAEARDGLLRHHNEYITATLQRPGARAFTTP
                     DEAVDKTAFAPGAVATIGTPDDLVERIKSVLEISGGFGTVVGFVHDWANPENTMRSWD
                     MVARYVVPEINGYVAKLRQSREFVANNREYFNRAREAVMAKITENEAAAAALAVTKSP
                     LLAASSGNVPDLDQARARLAPR"
     gene            5563..5973
                     /locus_tag="VNU03_02930"
     CDS             5563..5973
                     /locus_tag="VNU03_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWP73431.1"
                     /translation="MADAGDIVRESIKAWNAHDPDGVARLVSDDFVSESDTLPAALRG
                     PEAVRQAVQMYVHAFPDLHFDVEQILASGDHVTLRWVATGTHGGELMGIPPTNKRVVT
                     HGCTVEEVRNGKLVREWIYWDTGNLLRQIGVLPR"
     gene            complement(6001..>6446)
                     /locus_tag="VNU03_02935"
     CDS             complement(6001..>6446)
                     /locus_tag="VNU03_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWP73432.1"
                     /translation="TLDEALEASLLLQVVDAADPGFRAQMQVTREVLDEIGAGDVPWM
                     ILVLNKIDKVAPADREALSREFPAALLVSAKDPVDVGRVRDAIIGTFEADMTEVDLVV
                     PYDAGRAIGEIHKLHVISESYEGDGVHYRLRAPGPALERLRAILR"
BASE COUNT         1019 a         2251 c         2224 g          952 t
ORIGIN      
        1 ccgatgctgc tcctgccgct cggccatccg cgcgaccggg cccaggtctt ccccctgttc
       61 agctcggcgc gcaccttcgc ttcggtcacg aaatccgtcg agaccatcac cgtgggtggc
      121 caggtcgcgg acgtcatgcg ccgggccctg agcctgatgc gcatgggccg tcccggcccg
      181 gtgatggtgg agatcccggc cgacctcgcg aacgaggagg tgcccgaggc cctgatcgac
      241 gcgtaccggc cggtcaaggc gacgaccgcc ggcgccaatg cgcgcgacgt cgagacggcc
      301 gcccgcgtgc tccgcgaggc gcggcggccg gtgatccacg ccggccaggg cgtgctgtac
      361 gccgaagcgt cggccgatct ggtcgagctg gccgagctgc tccaggcccc cgtcatgacc
      421 acgctcgagg gcaagagcgc attcccggag gaccacccgc tcgccctcgg caccggcggc
      481 ccggtggtga ccggacacac cctgcagtat ctccgcgagg ccgacgtggt gttcggcatc
      541 gggtgcagct tcacccgcca cgggatggcg gccaccatcc ccgtgggcaa gaccctgatc
      601 cacgcgacca acgacgagcg cgacctcaac aagagctacg cggccgacta cccgctcctg
      661 ggcgacgcgc gcctcgtgct gcgccagttc atcgaggcga tcaagagcca gggtggccgc
      721 ccggccggcc gggagcaggt gcgcgcggac ctcgatcgcg tgcgcgcggc gtggctcgcg
      781 gagtggagcg cgacgctcgg ctcgaccgag gtcccgatga ccccctaccg ggtcatcgcc
      841 gagttcatgc gggcggtgga tccgcgcgag gccatcgtca cgcacgactc cgggagcccg
      901 cgcgaccagc tcctgccctt ctaccgggcg gtcccgccgc gcagctatct cggctggggc
      961 aagtcgcacg cgctcggcac cggcctcggc ctgatcatcg gggccaagct cgccgctccc
     1021 gagaagttct gcgtcaactt catgggcgac gccgccttcg gcatgaccgg gctcgatttc
     1081 gagaccgcgg tgcgctgcgg ggtcccgatc ctcaccgtcg tgctcaataa ctcggcgatg
     1141 gccatcgaga tcccgcacct ggtggtgtcg cacgacaagt acggcacgcg cgacatcggc
     1201 gggcgctacg cggacatggg ccgcgcgatg ggcggctgga gcgagcgggt cgagcggccc
     1261 gaggacatcg cgggcgcctt cgcgcgcgcc cgcaaggcca ccgagggcgg gcaggcggcg
     1321 ctgctcgagt tcatcaccag ccaggagacg cggttctcga atcgcggcgt gctccgatag
     1381 gaggcctcac atgacgcgca tcgcctactt caacggcaag ttcgtgcccg agaccgaggt
     1441 gctcatcccc taccgcgacc gcagcttcaa ccgcggcgac ggctgcttcg acatgacgcg
     1501 caccttcgac gggcgcatct tccgcctcga ggagcacgtg gagcggctct accgatcgct
     1561 gcgctacctg cgcatcgaca tcggcctcga gcccaaggag atcgtcgcga tcagcgagga
     1621 ggtcgtgctg cgcaacgagc ccctccgcgc cgccgcgggc gactggtggc tggcccagcg
     1681 ggtgagccgc ggggtcgacg cgatcggcga cgaggggtgg gcccacaccg ggccccaggt
     1741 catcgtcgac tgcaccccgc tgcccctcaa ggcgcgggcg gcccagttcc gggacggcat
     1801 cgacgtggtg gtgccgtcga tccgtcgcac tccaccgtcg acgctaagcc cgcgcgccaa
     1861 gacccacaac tacctgaacc tcatcctggc cgacaaggag gtgaaggccg cgcagccgca
     1921 ggcgtggtcg gtgctgctcg acgagaacgg caacctctgc gagggggtcg gcagcaacgt
     1981 cttcgtggtg cgcgacgagc ggatctacac gccgcgcgag aagtacgtcc tgcccggggt
     2041 cagccggcga accgcgatgg agctcggcac caagctcggc ctggcggtgg aggagcgcga
     2101 cctcgacgtc tacgacgcgg tcaccgccga cgaggtgttc ctgacctcga ccagcctgtg
     2161 cgtctgcccg gtccgctcgg tgaacggcca cgtgatcggc gacgggcgag tacccggacc
     2221 ggtgaccagg cggatcaccc aggcgtacat cgagcacgtg ggcaccgact tcgtggccca
     2281 gtacctgtcc cgactcggct gagggcggcg ccccctagat gacgtccagc cggcgagtcc
     2341 aggtccgagc cctcgccacc cttctactcg tcatcctgct cgccggtccg gcgttcgccc
     2401 aggcgccggc gtatccggga gcgacctgga cccggacgtc gtctcccgag cagctcggct
     2461 ggtcatcgga gaagctgaag gaggccaagg cctacgcggc cacgatcaac accgcggcgc
     2521 tcatggtcgt ggtcaatggc accgtgctcg acgagtgggg cgagaccgcg aagcgcttca
     2581 acgtccactc gatccggaag agcttcctca gcgcgctgta cggcatccag gtgcgggaag
     2641 gtcggatcca tctgtcaacg aacctcgccg atctcggcat cgacgatcgc ggttccttga
     2701 ccggcgtcga gaagggggcg acggtggcgg acctgctgaa ggcccgctcc gggatctatc
     2761 atccggcgct gtacgagacg ccggagatgg cggccggccg ccccgcgcga ggcagctact
     2821 cccccgggac gttctggtac tacaacaact gggacttcaa tgcgctaggc accatctacg
     2881 agcaggcggt gaaggcgtcc atcttcgagg agttcaagtc gcggatcgcc gacccgctcg
     2941 agatggagga cttccggatc gcggatggct cgtacgtccg gggcccggag tccatccacg
     3001 ccgcctatcc cttccggatg acggcgcgcg acatggcgcg cttcggtctc ctgtacctgc
     3061 ggggcggaca gtggcgtggc cgatccgtcg taccccggga atgggtggag gagagtaccc
     3121 gggccttctc cgacctcggc cagtcgggcg gctacggata tctctggtgg gtggctccga
     3181 acggcgggcc gcacttcacc tcgtcctact tcaacgggcg tgtcttctcg gcccgcggtg
     3241 ccggaggcca cttcatcgtg gtggttccgt atctggacct cgtggtggtc catcgcgtcg
     3301 atacggatgt ccagggccgg caggtgagcg gtgcgcagtt cggtcacctc atgcagctca
     3361 tcctcgacgc caggcgatag cccacccggc gggccccagg accgccgcca tgatctgatg
     3421 gcctagcctc ggcgggggat ggtaacgttg gggcacggaa gcggccgatc cgagggcggt
     3481 ccggcccatc attcaccacc caggagatcc gttcatgcca cgtctgaact tcggcgcctt
     3541 catcgccccc caccacccga tcggcgagca cccgatgctg cagttccgcc gcgatctcga
     3601 cctggtggag catctggaca agctcgggta cgaggaattc tggtgcggcg agcatcattc
     3661 cagcggctgg gagatgatcg cctcgcccga gatgttcctc gccgcggcgg ccgagcgctc
     3721 gaagcgcatc aagctcgcga ccggcgtgat ctcccttccg tatcaccatc cgttcaacgt
     3781 ggcgcagcgg atggtgcagc tcgactacat gaccggtggc cgggcgatct tcggctccgg
     3841 gccgggagcc ctggcgtcgg acgcgcacac cctcgcgatc gatcccatgc tcctccgcga
     3901 ccgccaggac gaggccatcg gggtcatccg ccgcctgttc cgcggcgagc gcgtctccta
     3961 caagtccgac tggttcacgc tccaggacgc ggccctgcag ctgctgccgc tgcaggagga
     4021 gatgccctgc gcggtcgcct cccagatcag cccctccggc atgaccctgg ccggaaaaca
     4081 tgggatcggc atcatctcga tcgggtcgct gtcggaggaa gggctgaacg ccctgcccac
     4141 gcagtggagc ttcgcggagg acgcggcggc caagcacggg cagaccgtcg accgcaagaa
     4201 ctggcgggtg ctgctgagct ggcacatcgc cgagacccgg gagaaggcgc gcgccgaggc
     4261 ccgcgacggc ctgctgcggc accacaacga gtacatcacc gcgaccctgc agcggcccgg
     4321 cgcgcgagcc ttcacgaccc ccgacgaagc ggtggacaag accgcgttcg cccccggcgc
     4381 cgtcgccacg atcggcaccc cggacgatct ggtggagcgg atcaagtcgg tcctcgagat
     4441 ctccggcggc ttcggcaccg tcgtcggctt cgtccacgac tgggcgaacc cggagaacac
     4501 gatgcggagc tgggacatgg tggcccgcta cgtggtgccc gagatcaacg gctacgtggc
     4561 caagttgcgc caatcccgcg agttcgtcgc caacaaccgc gagtacttca accgcgcccg
     4621 cgaggcggtg atggcgaaga tcaccgagaa cgaggccgcg gccgccgcgc tcgcggtcac
     4681 gaagtcgccg ctcctggccg cctcctccgg caacgtgccg gacctggacc aggcccgcgc
     4741 ccggctcgcg ccacgatgag gggcgccggc gagccctaga ctgcgctccc cgtcggattg
     4801 accgcgcccg ttttaccgag accgcggccg gtcgggcgcg aggctacggg aattccggag
     4861 atctaggcgg cttccagtgc ggcatgtccc ttgctgttca gcgaagcatg aaggcgaggg
     4921 gcgaggccgg ctggcgagtc ttcaggattc ggatccgact atgggagcgg ctgcgccgcc
     4981 tccacacgcc aacgggcgac ggatcgctga gcaccgaagc gaccagccgg cctcggcgga
     5041 gggccaccgc aaagggggac gccgcgaaac ctgaccggct gaccgtcctc gtcagccgca
     5101 aggttcccgg tcagctcggg caacctccgc caagctgatg ccgccccgat aggggcgtca
     5161 gggggaggcg cggtgggtgt gggcggccgt gtgagtcgcc agatcccgtc ttgcgagcgc
     5221 gatgcatcgg gggcacgtcg tcgccgcggg gtcggacgag acgccggtgc cgggcgactt
     5281 gcacaggggt cgctttcggc cggccgggag gaagtggacc cgtcgtccct tcatcgcgcg
     5341 aacaggctat cgcgaggggt cgcgaaaccc cccgagaatt ctgcggccgg ctgcgcgcca
     5401 gcgcggtacc cggcctccct cccttgacaa ggtttttgtg ccccgccata gtcatcggcg
     5461 cggttcccac ccatcaacgc ccggctcgtc ggggcacagg aggccccggg tcgagtccct
     5521 cacgttctgg ccgcaacccc gccaagagtc cggaggagcg acatggcaga cgctggcgat
     5581 attgtccgcg agagcatcaa ggcctggaac gcccacgatc ccgatggggt cgcgcggctc
     5641 gtatcggatg acttcgtctc cgagtcggac acgctgcccg cggccttgcg agggcccgag
     5701 gcggtccgac aagccgtgca gatgtacgtg cacgcgttcc ccgacctgca cttcgatgtc
     5761 gagcagatac tggccagtgg cgaccatgtg acgctgcgat gggtcgcgac cggcacccac
     5821 ggcggcgagc tgatgggcat tccgccgacg aacaagcgcg tggtgactca cggatgcacg
     5881 gtcgaggagg tcaggaacgg gaagctcgtc cgggaatgga tctactggga tacgggtaac
     5941 ttgctccggc agatcggggt ccttccgagg taacggggaa cggcggggcg cggggagacc
     6001 ctagcgcaga atcgcccgta gccgctcgag cgccggcccg ggggccctca gccggtagtg
     6061 cacgccgtcg ccctcgtagg actccgagat cacgtggagc ttgtggatct cgccgatcgc
     6121 gcgccccgcg tcgtagggga cgacgaggtc gacctcggtc atatccgcct cgaaggtgcc
     6181 gatgatcgcg tcccgcacgc gcccgacgtc gaccggatcc ttggccgaga cgagcagggc
     6241 ggcggggaat tcccgcgaga gcgcctcgcg atcggccgga gcgaccttgt cgatcttgtt
     6301 gaggacgagg atcatccacg gcacgtcgcc cgcgccgatc tcgtccagga cctcgcgggt
     6361 gacctgcatc tgggcccgga agccggggtc ggccgcgtcg acgacctgca ggagcaggga
     6421 cgcctcgagt gcctcgtcga gggtcg
//
LOCUS       DATMCV010000121         8156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_272795, whole genome shotgun sequence.
ACCESSION   DATMCV010000121 DATMCV010000000
VERSION     DATMCV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272795"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1405)
                     /locus_tag="VNU03_02940"
     CDS             complement(116..1405)
                     /locus_tag="VNU03_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWP73433.1"
                     /translation="MDSGVDGRLVPELIGLGALLLVSALLSGAEAAYFSLGRTRLKEL
                     AEQQGRTPGPLAPLLARPHELLVTLLVGITVVNIGASALAAAIAEQVFGSKGLGIAIA
                     GMVFLLTVFGEVLPMTLAVEHPLRFSAWVNRPVAWLSILLKPIRAGLGAFTAITLRLA
                     GSEHQAGRPEISEEELRTLVDVGAREGVVERSEREMIHRVFELEDTLVREVMVPRPDM
                     FCLDVTTETQRLLPLLREQLHSRVPVFEDTIDQIVGVLYTKDLLPYLDGLPPGFGLRQ
                     HLHPPYFVPGSKRADALLREFQAKKLHLAIVVDEYGGTAGLVTLEDLLEELVGEIRDE
                     YDEDERLIQRVAPRTFRVSGRLPIDELNAATGLAIPNESFDTVGGWVLDLFGRVPHKG
                     EKKESDGVRVAVEKVERTRVLEVLVTLPEVASRSEAA"
     gene            complement(1398..2453)
                     /gene="thiL"
                     /locus_tag="VNU03_02945"
     CDS             complement(1398..2453)
                     /gene="thiL"
                     /locus_tag="VNU03_02945"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWP73434.1"
                     /translation="MKASEPPERVGLGQLGERGLIRRIRRSGVATEGAPGIEVGIGDD
                     TAVMTVPPGHRLLATTDLLIEDVHFRPSSASPADIGWKALAVNLSDIAAMGGIPRWAL
                     VALAVPAGTEVAAVDGFYAGMGEAAAPHAVTVVGGDTSASPTGWMVNVTLLGVHPGAP
                     RLRSHARPGDAVAVTGSLGASAAGLYALEMGSDRAHAAGLTGGPLAEIVATHLRPRAR
                     VAEGRWLGEAAGVHAMMDCSDGLTTDLEHICRESGVGARVRLDRVPVAASALAAGRAL
                     GTDARDWAVGGGEDYELLLTCEAGAAERLASGLASATGTPLTIIGRIEGTAGSVVFVD
                     ADDEPVAVRSGYEHFHG"
     gene            complement(2450..3034)
                     /locus_tag="VNU03_02950"
     CDS             complement(2450..3034)
                     /locus_tag="VNU03_02950"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWP73435.1"
                     /translation="MILVLDNYDSFTYNLVQYMGELGATMHVARNDALTVDDVEALSP
                     AAIVISPGPGNPDGAGISLGLIERFHARVPILGVCLGHQAIGQAFGGTVARAGRQMHG
                     KTSAIAHDGRGVFRGLPAGFEATRYHSLVVLEAGLPADLEITARAEDGEIMGLRHRRH
                     PVEGVQFHPESILTTQGKALLGNFLELCSVAAPA"
     gene            complement(3031..4569)
                     /gene="trpE"
                     /locus_tag="VNU03_02955"
     CDS             complement(3031..4569)
                     /gene="trpE"
                     /locus_tag="VNU03_02955"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HWP73436.1"
                     /translation="MSTVGPGLPPLSPTREEFHALAAQGNLIPIFAELAADLDTPLSA
                     FLRLRPGPYAFLLESVEGGEKWARYSFLGTDPHLVFTAKGSRVSVRRSDGTTELLRSP
                     NPFETLRGLLAQFKPVPVPGLPRFQGGAVGFLSYDMIRHVERLPVLAKDDQKLPDAVF
                     MFTDSLLVFDNLRHRLLVIANAHIGKTDPASLDRAYDAAAVKIGMLLGNLRRPARSPA
                     PLTLPPVDPLVALGDEGFTSSMDQATFMDGVRRVKEFIAAGDAYQVVISRRLDTELRA
                     DPFTVYRALRTINPSPYLFFLRLGKTSIVGSSPEVLVRLEDDRVEERPIAGTHPRGRT
                     EEEDARLATEMRADPKERAEHVMLVDLGRNDVGRVSRIGSVEVSEFMVVERYSHVMHL
                     VSHVRGRLEPGKDAFDVLAACFPAGTLTGAPKIRAMEIIEEIEATRRGPYGGAVGYIS
                     YSGNLDSCITIRTVVCHGRRASIQVGAGIVADSDPKTEWLETCSKARGMILALRIAAQ
                     EPAR"
     gene            complement(4578..4904)
                     /gene="trxA"
                     /locus_tag="VNU03_02960"
     CDS             complement(4578..4904)
                     /gene="trxA"
                     /locus_tag="VNU03_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWP73437.1"
                     /translation="MAESTLHLTETSFDAELGKHNELMMVDFWAEWCAPCRAIAPTLE
                     ELVREGNGAVTLAKVNVDEQPALAARYGIRSIPTILFVKGGQVLDQVIGAVPKAQLKK
                     KLDANT"
     gene            5370..5732
                     /locus_tag="VNU03_02965"
     CDS             5370..5732
                     /locus_tag="VNU03_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73438.1"
                     /translation="MGKVMVVDDAVSELKVIESILKAAGHQVVSYQDGELLEDRVVEE
                     RPDVLLLDIVMPKRNGYEVLRGIRRDPRTKDTPVVFVSSKNQESDRLWGQRQGAADYL
                     AKPFTAEQLLGVVRQFVK"
     gene            5737..6261
                     /locus_tag="VNU03_02970"
     CDS             5737..6261
                     /locus_tag="VNU03_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWP73439.1"
                     /translation="MVDTPGAVALPSALPDSSPALRACLFALSGARFAVDVRSAREVA
                     VFDDLTVVPRAPRLLLGVANLRGTVVPIVDVGPLLGLPELRPARSVRTLVLRDGPLLA
                     AVAVETVLGLEPFDDVLSPDGPTAPQGHASPVLVSGWITWAGETLALLDVPRILAVLR
                     ASLGAAAPAGRSEA"
     gene            6258..7514
                     /locus_tag="VNU03_02975"
     CDS             6258..7514
                     /locus_tag="VNU03_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73440.1"
                     /translation="MNRRGFLGLLTAGVAGGVLAKPAAAAPGPSANEIRIGMSAAFRG
                     SAAGLGAELYRGAQAYYSEINARGGVFGRPIAVVALDDSYNPEPCIRNTIQLLDREKV
                     FFLSNYVGTPTLTRALPVIKQYADQNVVLVGNFTGAQPQREAPYVDQVFNVRASYRQE
                     MQALVERFWQAGARRFGVYYQVDAYGRSGTDGVARALAARGARIVAEATYVRNATFDE
                     DMGIAVTALREAGCDVVLCTGAYQGCAAFVRSARDAGFAVPISNVSFVGSDAMLALLV
                     KHGRAKGRDYTRGLINSQVVPSYDDTTLPGVAEYRALMDKHNPPVPEAIRDANYAPQK
                     YSFISLEGYVNARVITEALKRAGANAGRPGFRQALESLRGLDLGIGAPLTFTPERHQG
                     LDSVYFTRVDGDHWVPIVDWAAAVKV"
     gene            7558..>8156
                     /locus_tag="VNU03_02980"
     CDS             7558..>8156
                     /locus_tag="VNU03_02980"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73441.1"
                     /translation="MRKGADGGLPGGEHLVGSSPAHRVRFGLLGKITVFLILVLVPLA
                     AVSWTVAYRSLQDNLTREFTDKGSAIANSLASSGADLIVNRDASTVQAQVDEFAKITG
                     VAYVMVYDPQRGFIAHTFAPVVPKGIVEMNVVPGHAARPQVREITYPDPRTGRAREII
                     DIGVPMLGGQLGTVRVGMDKAIITAASVQAGNFLLLVFGG"
BASE COUNT         1207 a         2847 c         2904 g         1198 t
ORIGIN      
        1 ttcccgccct ccgccatgtg ccggaggcgg acccggttgg ccgcgatgaa cgacatctcg
       61 gcggccgaga agagcgcggt caccagcagg gagaggccga tgatccagac gtagatcacg
      121 ccgcctcgct ccgggaggcc acctcgggca gggtcaccag cacctcgagc acccgggtcc
      181 gctcgacctt ctcgaccgcg acccggacgc cgtccgactc cttcttctcg cccttgtggg
      241 gcacgcgccc gaagaggtcg aggacccacc ctcccacggt gtcgaacgat tcgttgggga
      301 tcgcgaggcc ggtggccgcg ttcagctcgt cgatgggcag gcggcccgag acgcgaaagg
      361 tgcgcggcgc cacccgctgg atgaggcgct cgtcctcgtc gtactcgtcg cggatctcgc
      421 ccaccagctc ctcgagcagg tcctcgaggg tcacgagccc ggcggtgccg ccgtactcgt
      481 ccaccacgat ggcgaggtgc agcttcttgg cctggaactc gcgcagcagg gcgtcggcgc
      541 gcttcgagcc cgggacgaag tacggcgggt gcaggtgctg tcggaggccg aagccgggcg
      601 gcaggccgtc gaggtagggc aggaggtcct tggtgtagag caccccgacg atctggtcga
      661 tggtgtcctc gaagaccggc accctcgagt ggagctgctc gcgcaggagc ggcaggagcc
      721 gctgagtctc ggtggtgacg tcgaggcaga acatgtcggg acgggggacc atcacctcgc
      781 gcacgagggt gtcctccagc tcgaacacgc gatggatcat ctcgcgctcg ctgcgctcca
      841 ccacgccctc gcgcgctccc acgtccacca gggtgcgcag ctcctcctcg gagatctccg
      901 gccgcccggc ctggtgctcg gagccggcga gcctcagggt gatggcggtg aacgcgccga
      961 gcccggcgcg gatcggcttc agcaggatcg agagccacgc caccggccgg ttcacccacg
     1021 ccgagaagcg gagcgggtgc tcgacggcca gggtcatggg caggacctcg ccgaagacgg
     1081 tgagcaggaa caccatgccc gcgatcgcga tgcccaggcc cttggagccg aagacctgct
     1141 ccgcgatggc ggccgcgagc gcggaggccc cgatgttcac caccgtgatc ccgaccagca
     1201 gggtcaccag cagctcgtga ggccgggcca ggagcggggc gagcgggccc ggcgtccgcc
     1261 cctgctgctc cgcgagctcc ttgagccgcg tccggccgag tgagaagtac gcggcctccg
     1321 cgcccgagag caaggccgac accaggagca gcgcgccgag cccgatcagc tccgggacga
     1381 ggcggccgtc cacgccgcta tccatggaaa tgctcgtagc cgctccgcac cgccaccggc
     1441 tcgtcgtcgg cgtccacgaa caccacgctc ccggcggtcc cctcgatgcg gccgatgatc
     1501 gtcagcggcg tcccggtcgc ggacgcgagg cccgaggcga gccgctcggc cgcgcccgcc
     1561 tcgcaggtca gcagcagctc gtagtcctcg ccgcccccga cggcccagtc gcgcgcgtcg
     1621 gtcccgaggg cccggcccgc ggccagcgcc gacgcggcca ccggcacgcg gtcgagccgc
     1681 acgcgcgcgc ccaccccgct ctcccggcag atgtgctcga gatccgtggt aagcccgtcc
     1741 gagcagtcca tcatcgcgtg cacgcccgcc gcctcgccaa gccagcggcc ctcggcgacg
     1801 cgggcccgcg gccgcaggtg ggtcgccacg atctcggcca gcgggccccc ggtgagcccg
     1861 gccgcgtgcg cccggtccga ccccatctcg agggcataga ggccggcggc ggacgcgccg
     1921 aggcttccgg tgactgccac cgcgtcgccc ggacgcgcgt gggacctgag ccgcggagcg
     1981 ccggggtgga cgccgagcag ggtcacgttc accatccagc cggtcgggga ggccgaggtg
     2041 tcgcccccca cgaccgtcac tgcatgaggc gcggccgctt cccccatgcc ggcgtagaac
     2101 ccgtcgaccg ccgcgacctc ggttccggcg gggacggcga gggccaccag cgcccatcgg
     2161 gggatgccgc ccatcgcggc gatgtcggag agattgaccg cgagggcctt ccatccgatg
     2221 tcggccggac tggcggagct cgggcggaag tggacgtcct cgatcaggag atcggtggtg
     2281 gcgagcaggc ggtggcctgg aggaaccgtc atgaccgccg tgtcgtcgcc gatcccgacc
     2341 tcgatccccg gcgccccctc ggtcgcgacc ccactccgcc tgatccggcg gatcagcccg
     2401 cgctcgccca gctgccccag acccacccgt tcgggtggct cggatgcctt catgcggggg
     2461 cggcgacgga gcagagctcg aggaaattcc ccagcagcgc cttgccctgg gtggtgagga
     2521 tggactcggg gtggaactgc acaccctcga cgggatggcg ccggtgacga aggcccatga
     2581 tctcgccgtc ctcggcccgc gcggtgatct cgagatccgc gggaagaccc gcctcgagga
     2641 ccaccagtga gtgatagcgc gtcgcctcga agccggcggg gagtccgcgg aagacgccgc
     2701 ggccgtcgtg cgcgatcgcc gacgtcttgc catgcatctg ccggcccgcg cgggccaccg
     2761 tgcccccaaa ggcctgcccg atggcctggt gacccaagca gacgcccagg atcggcaccc
     2821 gcgcgtggaa gcgctcgatc agcccgaggg agatgccggc cccgtcgggg ttgccgggcc
     2881 cgggcgagat cacgatcgcc gccggggaga gcgcctcgac gtcgtcgacg gtgagcgcgt
     2941 cgttgcgcgc cacgtgcatg gtggcgccga gctcgcccat gtactgcacg aggttgtagg
     3001 tgaacgagtc gtagttgtcg aggaccagga tcatcgcgcc ggctcctggg cggcgatccg
     3061 cagcgccagg atcatgccgc gggccttcga gcacgtctcg agccattccg tcttcggatc
     3121 cgagtcggcc acgatccccg ccccgacctg gatcgaggcc cgccgcccgt ggcataccac
     3181 cgtgcggatg gtgatgcacg aatccaggtt gccggagtac gagatgtagc cgaccgcgcc
     3241 gccgtacggg ccgcgccggg tcgcctcgat ctcctcgatg atctccatcg cccggatctt
     3301 gggcgctccg gtgagggtgc cggccgggaa acaggccgcg agcacgtcga aggcgtcctt
     3361 gcccggctcg aggcggcccc gcacgtggct caccaggtgc atcacgtgcg agtagcgctc
     3421 caccaccatg aactccgaca cctcgacgga gccgatgcgg ctgacccggc cgacgtcgtt
     3481 gcgtcccagg tcgaccagca tgacgtgctc ggcgcgctcc ttggggtcgg cgcgcatctc
     3541 ggtcgcgagc cgggcgtctt cctcctcggt ccgcccgcgc ggatgcgtgc ccgcgatggg
     3601 ccgctcctcc acccgatcgt cctcgaggcg taccagcacc tccggcgaag accccacgat
     3661 gctggtcttg ccgaggcgaa ggaagaacag gtacggcgag gggttgatgg tgcggagcgc
     3721 gcgatagacc gtgaacggat cggcgcgcag ctcggtatcg agccgtcgcg agatcacgac
     3781 ctgataggca tcgccggccg cgatgaactc tttgacccgg cggaccccgt ccatgaaggt
     3841 cgcctggtcc atgctcgagg tgaacccctc gtcgcccagg gccaccaggg gatccaccgg
     3901 gggcagcgtg agcggggcgg gggagcgggc cggccgtctc aggttgccca ggagcatgcc
     3961 gatcttcacc gcggccgcgt cgtaggcccg gtcgagggag gccgggtcgg tcttgccgat
     4021 atgggcgttg gcgatcacga gcagtcggtg gcgcaggttg tcgaagacca gcaggctgtc
     4081 ggtgaacatg aacaccgcgt cggggagctt ctggtcgtcc ttggccagga ccgggaggcg
     4141 ctcgacgtgg cggatcatgt cgtaggacag gaacccgacc gcgccgccct ggaatcgcgg
     4201 caggcccggc accgggaccg gcttgaactg agccagcagg ccgcgcaggg tctcgaacgg
     4261 gttggggctc cgcagcagct ccgtggtccc gtcggaccgc cgaacgctga cccggctccc
     4321 cttggcggtg aacacgaggt gcgggtcggt ccccaggaac gagtagcggg cccacttctc
     4381 gccaccttcg accgactcga ggaggaaggc gtaggggccg ggccggagcc gcaggaaggc
     4441 ggacagggga gtgtcgaggt cggccgcgag ctcggcgaag atcgggatca ggttgccctg
     4501 ggccgctagg gcgtgaaact cctcacgcgt cggtgagagc gggggcagac caggccccac
     4561 cgtcgacatg gggtcgctca ggtgttggcg tccagcttct tcttgagctg ggccttgggc
     4621 acggcgccga tcacctgatc gagcacctgg ccacccttga cgaacaggat ggtcgggatc
     4681 gaccggatgc cgtagcgagc cgccagcgcc ggctgctcgt ccacgttcac cttcgccagg
     4741 gtcacggcgc cgttgccctc gcgcaccagc tcctcgagcg tcggggcgat ggcccggcag
     4801 ggcgcgcacc actccgccca gaagtccacc atcatgagct cgttgtgctt gcccagttcg
     4861 gcgtcgaagc tggtctcggt gagatggagc gtggactccg ccatggatcg agggtctcct
     4921 tgtcagaggg cctgcaggac ggcgggtgag agttccataa aaaacgcacg gctacgctag
     4981 catacggccc tggtcatcgc aagttgggcg cccgcgccgg cctccgcgag gctcacagcg
     5041 caggttgcgg tgaccaagat cgctgcccag tgggctgggc ggccgaccgt caggggcacc
     5101 tctcctgaca gtgagcgtca ctggacggac gtccaccggt tgtcactgaa ggggccgagg
     5161 gccacaaaac gggcccggac agcccgaaag ccttgaatcc atgacatctc cttgtgcccc
     5221 ttgcacatgc cgtccaagcc ggtagaaaac ctcggttggt agggggtttg cagtagatgc
     5281 atccccctgc gtagccccca gtgggccccc tgttgcgggc cggggccgca ggtgagcagg
     5341 tgcgaacctc agctggggag gatgagctct tgggcaaggt catggtggtg gatgacgcgg
     5401 tgtcggagct gaaggtgatc gagagcattc tcaaggccgc cggccaccag gtggtctcct
     5461 atcaggacgg cgagctgctg gaggatcgcg tggtggagga gcggcccgac gtgctcctcc
     5521 tggacatcgt gatgcccaag cgcaacggct acgaggtgct gcgcggcatc cgtcgtgatc
     5581 cgcgcaccaa ggacaccccg gtcgtcttcg tgagctcgaa gaaccaggag agcgatcggc
     5641 tgtggggtca gcggcagggc gccgccgact acctggccaa gccgttcacc gccgagcagc
     5701 tcctcggcgt ggtccgccag ttcgtgaagt aggctgatgg tcgacactcc gggcgccgtc
     5761 gcgctcccgt ccgcgcttcc ggactcgtcg ccggcgctcc gcgcctgcct gttcgctctc
     5821 tcgggcgccc gattcgcggt ggacgtgcgc agcgcgcgcg aggtggccgt gttcgacgac
     5881 ctcaccgtgg tgccgcgcgc gccacgcctc ctgctcgggg tggccaacct gcggggtacg
     5941 gtggtgccga tcgtcgacgt gggtcccttg ctcggtctcc ccgagcttcg cccggctcgg
     6001 agtgtacgca cgctcgtcct gcgcgacggc cctcttctcg ccgcggtcgc ggtggagacc
     6061 gtgctcgggc tcgagccgtt cgacgacgtg ctctcgccgg acggccccac cgctccgcag
     6121 ggccacgcct cccccgtgct cgtgtcggga tggatcacgt gggccggcga gacgctggcc
     6181 ctgctggatg ttccgcggat cctcgccgtg ctccgagcct cgctcggcgc ggccgctccc
     6241 gcgggaagga gcgaggcatg aaccgccgag gctttctcgg tctcctcacc gccggcgtgg
     6301 cgggaggtgt gctggccaaa cccgcggccg cggccccggg cccctccgcg aacgagatcc
     6361 ggatcgggat gagcgcagcg ttccgcggct ccgccgccgg gctcggcgcc gagctgtacc
     6421 gcggagccca ggcctactac agcgagatca acgcgcgggg cggggtcttc gggcgcccga
     6481 tcgcggtggt ggcgctcgac gacagctaca accccgagcc ctgcattcgc aacacgatcc
     6541 agctgctcga tcgtgagaag gttttcttcc tctccaacta cgtggggacc ccgacgctca
     6601 cgcgcgccct gccggtgatc aagcagtacg cggatcagaa cgtggtcctg gtcggcaact
     6661 tcaccggcgc gcagccccag cgcgaggcgc cctatgtcga ccaggtcttc aacgtgcggg
     6721 cctcctatcg ccaggagatg caggcgctgg tggagcgttt ctggcaggcg ggtgcgcgtc
     6781 gcttcggcgt ctactaccag gtcgacgcct acggccgaag cggcaccgac ggcgtcgcgc
     6841 gggcgctcgc ggcgcgcggc gcccggatcg tcgcggaggc gacctacgtc cgaaacgcga
     6901 ccttcgacga ggacatgggg atcgcggtca ccgccctccg tgaggccggc tgtgacgtgg
     6961 tgctctgcac cggcgcctac cagggctgcg ccgccttcgt gcgctccgcg cgcgacgccg
     7021 gcttcgcggt accgatctcc aacgtctcgt tcgtgggctc ggacgccatg ctcgccctgc
     7081 tcgtgaagca cgggcgcgcc aagggccgcg actacacccg ggggctcatc aactcccagg
     7141 tggtgccgag ctacgacgac accaccctgc ccggggtcgc cgagtaccgg gccctgatgg
     7201 acaagcacaa ccctccggtg ccggaggcga ttcgcgacgc caactacgcg cctcagaaat
     7261 acagcttcat cagcctcgag ggttacgtca acgcgcgggt catcaccgag gcgctgaagc
     7321 gcgcgggcgc caacgcgggc cggccgggct tccgccaggc gctcgagtcg ctgcgcgggc
     7381 tcgatctggg gatcggcgcg ccgctgacgt tcacgcccga gcgtcaccag ggcctggaca
     7441 gcgtgtactt cacgcgggtg gacggcgacc actgggtgcc gatcgtggac tgggcggcgg
     7501 cggtgaaggt ctgagggcgg gatgaacgcc gcgaagcggg tctggcggag gcgtgccatg
     7561 agaaaggggg cggacggtgg ccttcccggt ggggagcatc tcgtcggctc gagcccggcg
     7621 catcgcgtgc gcttcggtct tctcggcaag atcacggtct tcttgatcct ggttctggtg
     7681 cctctcgccg cggtgagctg gacggtggcg taccggtcgc tgcaggacaa cctgacccgg
     7741 gagttcaccg acaagggcag cgcgatcgcc aacagcctgg ccagctccgg cgcggatctc
     7801 atcgtcaacc gcgacgcgtc caccgttcag gcccaggtgg acgagttcgc gaagatcacc
     7861 ggtgtcgcct acgtgatggt ctacgatccg cagcgcgggt tcatcgccca caccttcgcc
     7921 ccggtggtgc ccaagggcat cgtcgagatg aacgtggtcc ccgggcacgc cgcgcgcccg
     7981 caggtgcggg agatcaccta tccggatccc cggaccggcc gggcgcgcga aatcatcgac
     8041 atcggggtgc ccatgctggg cgggcagctg ggcacggtgc gggtgggcat ggacaaggcg
     8101 atcatcaccg ccgcgtcggt gcaggcgggc aacttccttc tgctggtgtt cggcgg
//
LOCUS       DATMCV010000122         2227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_739452, whole genome shotgun sequence.
ACCESSION   DATMCV010000122 DATMCV010000000
VERSION     DATMCV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739452"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..483)
                     /locus_tag="VNU03_02985"
     CDS             complement(1..483)
                     /locus_tag="VNU03_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWP73442.1"
                     /translation="MGYVERHLLAGERVVYKTRLHWALFLKPALVVLGGIALMVVLQR
                     VQDPPWLWMIGAGVTLVGLGWGLVHYVELMTSEFAVTSSRLILKVGLISRYTTELLLT
                     KVETIGVQQGLMGRMLGYGDLTVTGTGGAREVFRRVRDPIGFRNHVQQASIGPPPAAG
                     "
     gene            complement(501..1007)
                     /locus_tag="VNU03_02990"
     CDS             complement(501..1007)
                     /locus_tag="VNU03_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004934393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWP73443.1"
                     /translation="MIRSVPGRTPRVHPEAYVDPAAQVIGDVTIEAGASVWPFTVLRG
                     DQDNYVTLGRNSNVQDNSVLHVTPQFPCVVGANVTIGHRAVVHACTVMDNVRIGIGAV
                     VLTGAVVEEGAQVGAGALVPPGKVVPAGWLVMGVPARPVRKMSQDELDDILRNATEYL
                     DLWHRDYR"
     gene            complement(1004..1786)
                     /locus_tag="VNU03_02995"
     CDS             complement(1004..1786)
                     /locus_tag="VNU03_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWP73444.1"
                     /translation="MPTQDETLVTRDGPVVTLTFNRPEARNALTWGMYDRLYQVCEEV
                     DADDSVRVLVLRGAGGKAFVAGTDISQFKVFDRAEDGIAYERDGDKRIARLETVRKPV
                     IAQVQGFAVGGGFRIATACDLRIATPDARFGAPIARTLGNCLSMAAYASFVDVFGPSR
                     LKELIFTARLLTAEEAHAAGYVHEIVAAAEIEARVAALAHQLAEHAPITLWVTKESIR
                     RLAASRALPDDEDLIATTYMSADFREGVRAFLDKRKARWTGK"
     gene            1862..>2227
                     /locus_tag="VNU03_03000"
     CDS             1862..>2227
                     /locus_tag="VNU03_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73445.1"
                     /translation="MPRPAGLILDYGNVLSHAQDQTWMDEAARRLGGDADAFRAAYWQ
                     HRHAYDANLPAAAYWQRVAAATRPGALAPGDLAWLIASDVASWTVYHDEVWALAARFR
                     AQGGHTAFLSNSGPETMARV"
BASE COUNT          315 a          842 c          760 g          310 t
ORIGIN      
        1 tcagcccgcc gcggggggcg gcccgatcga ggcctgctgc acgtgattgc ggaagccgat
       61 cgggtcgcgc acccggcgga agacctcgcg ggcgccgccg gtgccggtca cggtcaggtc
      121 cccgtagccg agcatgcggc ccatcaggcc ctgctgcacg ccgatggtct ccaccttcgt
      181 cagcagcagc tcggtggtgt agcgcgagat caggccgacc ttcaggatga gccggctgct
      241 cgtcaccgcg aactcggacg tcatcagctc gacgtagtgg acgagccccc acccgagccc
      301 gaccagcgtg acgccggcgc cgatcatcca gagccagggc gggtcctgca cgcgctgcag
      361 caccaccatc agggcgatcc cgcccagcac cacgagggcc ggcttcagga acagcgccca
      421 gtgcagccgc gtcttgtaca ccacgcgctc gcccgccagc agatgccgct cgacgtaccc
      481 cacggctaga gcctccctcc ctaccggtag tcgcggtgcc agagatcgag gtactcggtg
      541 gcgttgcgga ggatgtcgtc gagctcgtcc tggctcatct tgcgcaccgg cctggccggc
      601 acccccatga cgagccagcc cgccggcacc accttgccgg gcggcacgag cgcgcccgcc
      661 cccacctgcg cgccctcctc caccaccgcg ccggtcagca ccaccgcgcc gatgccgatc
      721 cgcacgttgt ccatcacggt gcacgcgtgg accaccgcgc ggtgaccgat cgtcacgttg
      781 gccccgacga cgcacgggaa ctggggcgtg acgtgcagca ccgagttgtc ctgcacgttg
      841 gaattgcggc cgagcgtgac gtagttgtcc tggtcgcccc gcagcaccgt gaagggccac
      901 acgctcgcgc ccgcctcgat ggtcacgtcg ccgatgacct gggccgccgg gtccacgtag
      961 gcctccgggt gcacccgcgg cgtgcgcccc ggcaccgagc ggatcatttg ccggtccacc
     1021 gagccttgcg cttgtcgagg aacgcgcgaa ccccctcgcg gaagtcggcg ctcatgtagg
     1081 tggtggcgat caggtcctcg tcgtccggca gggcccggct cgcggccaga cggcggatcg
     1141 actccttggt cacccagagc gtgatcggcg cgtgctcggc cagctggtgg gcgagcgcgg
     1201 ccacccttgc ctcgatctcg gccgcggcca cgatctcgtg cacgtagccg gccgcgtgcg
     1261 cctcctcggc ggtgagcagg cgcgcggtga agatcagctc cttcaggcgc gagggcccga
     1321 acacgtcgac gaagctcgcg taggcggcca tcgagaggca gttgccgagg gtgcgcgcga
     1381 tcggggcccc gaagcgcgcg tcgggggtgg cgatgcgcag gtcgcaggcg gtggcgatgc
     1441 ggaagccgcc ccccaccgcg aagccctgga cctgggcgat caccggcttg cgcaccgtct
     1501 cgaggcgcgc gatgcgcttg tcgccgtcgc gctcgtaggc gatgccgtcc tcggcgcggt
     1561 cgaagacctt gaactggctg atgtccgtcc ccgccacgaa cgccttgccg cccgcgccgc
     1621 gcagcacgag cacgcgcacc gagtcgtcgg catccacctc ttcgcagacc tggtagagcc
     1681 gatcgtacat gccccaggtc agggcgttgc gcgcctcggg gcgattgaag gtcagcgtga
     1741 ccaccggccc gtcgcgggtc acgagggtct cgtcttgcgt cggcacggcg cgcgcctcct
     1801 ccgggagcag cgatgagaat cgccgcatcg taacacgccc gtctcgcgta gcatcgagcg
     1861 catgcctcga cccgccggcc tcatcctcga ctacggcaac gtgctgagcc acgcgcagga
     1921 ccagacgtgg atggacgagg cggcgcgccg gctcggcggc gacgccgacg ccttccgggc
     1981 cgcctactgg cagcaccggc acgcctacga cgcgaacctc ccggccgccg cgtactggca
     2041 gcgcgtggcg gccgccaccc gcccgggcgc gctcgcgccc ggcgacctgg cgtggctgat
     2101 cgcctccgac gtcgcctcgt ggaccgtcta tcacgacgag gtctgggcgc tcgcggcccg
     2161 cttccgcgcc cagggcgggc acaccgcgtt cctctccaac agcggtccgg agaccatggc
     2221 gcgcgtg
//
LOCUS       DATMCV010000123         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_407648, whole genome shotgun sequence.
ACCESSION   DATMCV010000123 DATMCV010000000
VERSION     DATMCV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407648"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VNU03_03005"
     CDS             complement(<1..879)
                     /locus_tag="VNU03_03005"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWP73446.1"
                     /translation="MIRARIVGIGAYAPKRILTNAELEKMVDTSDEWIVQRTGIRERH
                     IVEEGEGPADLGIRAAQQAMERAQVTPEEIDFIVVGTTAGDMHFPTTANLVQHRLGCR
                     NAGSVDVYAACSGSVYSLSMGAQYVQTGKYRTVLCIGAECLSRITDFTDRGTCILLAD
                     AAGAVVLRPATDDNGIIDTDLYSDGRYGELLIQPAGGSLRPATHETVDQRLHFAKMKG
                     NEVFKVAVRMFGDCAERILKNNGFTANDLDLFVPHQANLRIIEAAVKRLKVPMAKVMV
                     NVERYGNTGAASVYVAL"
     gene            complement(876..1901)
                     /gene="plsX"
                     /locus_tag="VNU03_03010"
     CDS             complement(876..1901)
                     /gene="plsX"
                     /locus_tag="VNU03_03010"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWP73447.1"
                     /translation="MKIAVDAMGGDFGPAVVVEGAVAACREFGLASVLVGDRAAIERE
                     IARLGAQDLPVSVRHASQVVGMAEAPSQALRRKRDSSLRVAAELVKEGECSALVSAGN
                     TGAAMAIGMYVMGVLPGVDRPAIAAPLPSLSGYTVLIDAGANVDPKPRQLFQFAVMGH
                     VYSRHIVGKDNPRVGLLSVGEEEGKGTDLTKEAFEDLRGSSLNFIGNIEGRDIYNGRC
                     DVVVTDGFTGNVCLKVSESLAEMLTAMIREELTRDILSVAGAALSKRAFARLKRRVDY
                     TEMGGAPLLGMNGAAIICHGASPVKAIKNAVRVAAEWVRNDVNQHIKTALEAEAVLAE
                     GREGGRE"
     gene            complement(1943..2125)
                     /gene="rpmF"
                     /locus_tag="VNU03_03015"
     CDS             complement(1943..2125)
                     /gene="rpmF"
                     /locus_tag="VNU03_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWP73448.1"
                     /translation="MPLPKRRHSKTRGRKRRTHYKMVAPSRSLCPQCREVKPPHRICP
                     HCGFYKGREVISVEGE"
     gene            complement(2138..2647)
                     /locus_tag="VNU03_03020"
     CDS             complement(2138..2647)
                     /locus_tag="VNU03_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HWP73449.1"
                     /translation="MLIQVSQIPDEGLRIEGVAEFPQPFQDATWALDELSLVVEKDGD
                     VVFVRGDLEARVPLHCGRCLEFYTVTVHPTVDARFVPAPASRSEERELARDDLETDVY
                     QHGQLDVTALLETETTLALPMKPLCREGCRGLCPVCGGNRNITACACETPALDSRWAA
                     LKDWAERTR"
BASE COUNT          410 a          958 c          918 g          445 t
ORIGIN      
        1 gagcgccacg tacaccgacg cggcgccggt attgccgtag cgctccacgt tcaccatcac
       61 cttcgccatc ggcaccttca ggcgcttgac cgccgcctcg atgatgcgga ggttcgcctg
      121 gtggggcacg aagagatcga gatcgttggc ggtgaagccg ttattcttga ggatgcgctc
      181 ggcgcagtcc ccgaacatcc gcaccgccac cttgaacacc tcgttaccct tcatcttggc
      241 gaagtgcagg cgctggtcca ccgtctcgtg ggtggcgggg cgcaacgagc cgcccgcggg
      301 ctggatgagc agctcgccgt agcggccgtc cgagtagaga tcggtgtcga tgatgccgtt
      361 gtcgtcggtg gcgggccgga gcaccaccgc gcccgccgcg tcggcgagca ggatgcaggt
      421 gccgcgatcg gtgaagtcgg tgatgcgcga gaggcactcg gccccgatgc agagcacggt
      481 gcggtacttg ccggtctgca cgtactgggc gcccatggag aggctgtaca ccgagcccga
      541 gcacgccgcg tagacgtcga ccgagcccgc gttgcggcag ccgaggcgat gctgcaccag
      601 gttggcggtg gtcgggaagt gcatgtcccc cgcggtggtg cccaccacga tgaagtcgat
      661 ctcctccggg gtgacctggg cgcgctccat cgcctgctgg gcggcccgga tgccgaggtc
      721 cgccggcccc tccccctcct cgacgatgtg gcgctcgcgg atgccggtcc gctggacgat
      781 ccactcgtcg gaggtgtcca ccatcttctc gagctccgcg ttcgtgagga tgcgcttggg
      841 cgcgtaggct ccgatgccga cgatgcgggc gcggatcatt cgcggcctcc ttcccgaccc
      901 tcggcgagga ctgcctcggc ctcgagcgcg gtcttgatgt gctggttcac gtcgttgcga
      961 acccactccg ccgccacccg caccgcgttc ttgatcgcct tcaccggcga ggcgccgtgg
     1021 cagatgatcg cggcgccgtt catgccgagc agcggggccc cgcccatctc ggtgtagtcg
     1081 acccgccgct tgagccgcgc gaacgcgcgc ttggacagcg cggcgcccgc gaccgacagg
     1141 atgtcgcggg tcagctcctc gcggatcatc gcggtgagca tctcggccag gctctcggag
     1201 accttgaggc agacgttgcc ggtgaagccg tcggtcacca ccacgtcgca gcgcccgttg
     1261 tagatgtcgc gcccctcgat gttgccgatg aagttgagcg acgagccgcg caggtcctcg
     1321 aaggcttcct tggtgaggtc ggtgcccttg ccctcttcct cgcccaccga gagcagcccc
     1381 acccggggat tgtccttgcc cacgatgtgg cgcgagtaca cgtggcccat caccgcgaac
     1441 tggaagagct gccgcggctt gggatccacg ttggcgcccg cgtcgatcaa caccgtgtag
     1501 ccggagagac tcggcagcgg cgccgcgatc gcggggcgat ccaccccggg cagcactccc
     1561 atcacgtaca tgccgatcgc catcgcggcg ccggtgttcc ccgccgagac gagcgcgctg
     1621 cactcgccct ccttcaccag ctcggccgcc acccgcagcg acgagtcgcg cttgcgacgc
     1681 agggcctgcg agggcgcctc cgccatgccc accacctgcg aggcgtggcg caccgagacc
     1741 ggcaggtcct gggcgccgag gcgggcgatc tcgcgctcga tggccgcgcg gtcgcccacc
     1801 agcaccgagg cgaggccgaa ctcgcggcac gcggccaccg ccccctcgac cacgacggca
     1861 gggccgaaat cgcctcccat ggcgtcgacc gcgatcttca tggcgccgat cttatggcac
     1921 ggctcggtcc agccggaaag aactattcgc cctcgaccga gatgacttcg cgccccttgt
     1981 agaagccgca gtgggggcag atgcggtggg gtggcttgac ctcgcggcac tgcgggcaga
     2041 gcgagcggct cggggcgacc atcttgtagt gcgtacgccg cttgcggccc cgcgtcttcg
     2101 aatggcgtcg cttgggaaga ggcatcgaat ctcctatcta tctcgtccgc tcggcccagt
     2161 ccttgagggc cgcccagcgc gaatcgagcg cgggcgtctc gcacgcgcag gccgtgatgt
     2221 tgcggttgcc gccgcacacg gggcacaggc cccggcagcc ctcacggcag aggggcttca
     2281 tgggcagcgc cagggtggtc tcggtctcga gcagggccgt cacgtcgagc tggccgtgct
     2341 ggtagacgtc cgtctccagg tcatcgcgag ccagctcccg ctcctccgag cggctggccg
     2401 gggcgggcac aaagcgggca tcgaccgtcg ggtgcaccgt gaccgtgtag aactcgaggc
     2461 accggccgca gtggagcggc acccgggcct ccagatcgcc acgcacgaag acgacatcgc
     2521 catctttctc caccaccagc gacagctcgt cgagcgccca cgtcgcgtcc tggaagggct
     2581 gcggaaactc cgcgacgccc tcgatacgca gaccctcgtc ggggatctgg gagacctgga
     2641 tcagcacgtg aaggacccct aactttctgt caattaaggc gttttgctat ggacgcgtca
     2701 gtatatgcgc ggggttaccc cttgtcaacc a
//
LOCUS       DATMCV010000124         3284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_873104, whole genome shotgun sequence.
ACCESSION   DATMCV010000124 DATMCV010000000
VERSION     DATMCV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873104"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VNU03_03025"
     CDS             complement(<1..364)
                     /locus_tag="VNU03_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73450.1"
                     /translation="MSAELLRQVRRVETQAEKGFDGGAAELVERRRRLRLEVLRQHLA
                     SNVDFLRAAHLGGASGQQSVQAYAAFMDGFLGTLYRLAVDDARREGLTATPLVLVALG
                     GYGRGELHPLSDLDLMVIY"
     gene            451..1557
                     /gene="hisC"
                     /locus_tag="VNU03_03030"
     CDS             451..1557
                     /gene="hisC"
                     /locus_tag="VNU03_03030"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWP73451.1"
                     /translation="MRRLWRSVLDQVATYDAGKSLETVARELGVDSVLRLSANENPLG
                     PSPRVIEAIRREAPRIHLYPDGGCTEVRQALASRLGIGPESIVVGNGADELLEMLARA
                     AFDPGDEVLIPHPAFEPYGTEATLSGATVVTSPLRGYDQDLDDMRSRVTPRTKCVILC
                     TPHNPAATIVRRRPLERFLDSLGTDPPLVILDEAYRDFCEDDDTPDGVVLLQRYPSLL
                     SLRTFSKIAGLAGLRVGYAIGRPEHIERLNRVRAPYNVNRLAQVAAVAALGDPEHLER
                     TRRVVLEQRPLLQAALAERGAPSPPSQANFILAKAGERADALRVALFQAGILVRDGAG
                     VGFPGHLRIAVGTAEQNARLLEVWDRSGRSAGRA"
     gene            complement(1649..1981)
                     /locus_tag="VNU03_03035"
     CDS             complement(1649..1981)
                     /locus_tag="VNU03_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73452.1"
                     /translation="MKPDGMALPATRSLASRLALRIRPRRPWMLIGSCLLLVVLTVTL
                     WGKWVESRDRADRLSAELKKVYVEAEKLRTEGAAAKQRITQLERQLRGRGARATAPAP
                     SGAPTDRR"
     gene            2054..2605
                     /locus_tag="VNU03_03040"
     CDS             2054..2605
                     /locus_tag="VNU03_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73453.1"
                     /translation="MQTGLLPRFFESVTRQAFGDLAIDDAPALRYLSDLLTRFVRTEA
                     LRMIRELPGRRLDSVTDSLLAIQHVWAADTPGFNPGTERVLRRQIGDYALFMTGIFRD
                     HVDRLAVTGYYEDEGRRAYRFVAELARGGAGSDASLYRRLADRFEMYAGALNYARKVY
                     FRADAAPVAAWPSGPFFRALLSE"
     gene            2602..>3284
                     /locus_tag="VNU03_03045"
     CDS             2602..>3284
                     /locus_tag="VNU03_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73454.1"
                     /translation="MTAAAPSDAAAACLAVLEAALGDATPPALPAGRADDLDEALRAL
                     ARRHGAAAVPLLERLAEAAPDKEHRRAVRRALYRLEQSGQRPARAPAATPARPVVARE
                     AEHPVRAWLSGIDGSGSRAAWILFEGGLGGGLRLCSLILNDEAGIMDAAGGPIARKRL
                     EAELRGLRESQKLPWVEVDGARACALVGQAIELHERLGTVPPPEFARWRKSFSGSSTV
                     SSLSGSSTVS"
BASE COUNT          434 a         1192 c         1203 g          455 t
ORIGIN      
        1 cgtagatcac catgaggtcg aggtcggaca gcgggtgtag ctcaccgcgc ccgtagccgc
       61 cgagcgccac cagcacgagg ggggtcgcgg tcagcccttc gcgccgggca tcgtccaccg
      121 ccaggcgata gagggtgccg aggaagccgt ccatgaaggc ggcgtaggcc tggaccgact
      181 gctggcccga cgcgccgccc aggtgcgcgg cgcgcaggaa gtccacgttg gaggcgagat
      241 gctggcgcag cacctcgagc cgcagccggc gccggcgctc gaccagctcg gccgcgccgc
      301 cgtcgaatcc cttctcggcc tgcgtctcga cccggcggac ctgtcggagc agttccgcag
      361 acatggcgaa acggtagcgt ggagcgagaa gaaagtcaaa cttggcgcga acttgtatcg
      421 cagtaactac cgcagttaca atggcgccct atgcgacgac tctggcgaag cgtactcgat
      481 caggtggcga cctacgacgc gggcaaatcg ctcgagacgg tggcgcgcga gctcggcgtg
      541 gactccgtgc tgcgcctgtc ggccaacgag aacccgctcg gcccctcgcc acgggtgatc
      601 gaggccatcc ggcgcgaggc gccacgcatc catctctatc ccgacggtgg ctgcacggaa
      661 gtgcggcagg cgctggcgag ccggctcggc atcggccccg agtccatcgt ggtcggcaac
      721 ggggccgacg agctcctgga gatgctcgcg cgggccgcat tcgacccggg cgacgaggtg
      781 ctgattcctc acccggcctt cgagccctac ggcaccgagg cgacgctctc gggcgccacc
      841 gtggtcacga gcccgctgcg cggctacgac caggacctcg acgacatgcg cagccgcgtc
      901 acgccgagga cgaagtgcgt gatcctctgc accccgcaca acccggccgc caccatcgtg
      961 cggcgtcggc ccctcgagcg cttcctggac tcgctgggca ccgatccgcc gctggtgatc
     1021 ctggacgagg cctaccggga cttctgcgaa gacgacgaca ccccggacgg cgtggtgctg
     1081 ctgcagcgct acccgagcct gctctcgctg cggacgttct ccaagatcgc ggggctggcc
     1141 gggctgcggg tgggctacgc gatcgggcgg cccgagcaca tcgaacggct gaaccgggtg
     1201 cgcgcgccct acaacgtcaa ccgcctggcc caggtggcgg cggtggccgc gctgggcgat
     1261 cccgagcatc tcgagcgcac gcgccgggtg gtgctggagc agcggcccct cctgcaggcc
     1321 gcgctggccg agcgcggcgc gccctcgccc ccctcgcagg ccaacttcat cctggccaag
     1381 gccggcgagc gggcggacgc gctgcgcgtg gcgctcttcc aggccggcat cctggtgcgg
     1441 gacggcgcgg gggtgggctt ccccgggcac ctgcgcatcg cggtcgggac cgcggagcag
     1501 aacgcgcggc tgctggaggt gtgggaccgc tccggcaggt cggccggtcg ggcgtagggc
     1561 ggcgcgctct ttcttcagct gcgggggccc agaaatggcc cccgcactcc cccattggcg
     1621 gacaaccgtt ttaggcttcg gcggtgctct agcggcggtc ggtcggggcg cctgaagggg
     1681 ccggagcggt ggcgcgggcg ccgcggccgc ggagctggcg ctcgagctgg gtgatgcgct
     1741 gcttggcggc ggcgccttcg gtgcgcagct tctcggcctc cacgtagacc ttcttcagct
     1801 ccgcggacag gcgatcggcc cggtcgcggc tctccaccca cttcccccac agggtcacgg
     1861 tcagcaccac cagcagcagg caggagccga tcagcatcca cggccggcgc gggcggatcc
     1921 ggagggcgag gcgggaggcg agggatcggg tcgcggggag ggccatgcca tccggcttca
     1981 tgcccggaag cctaccaccc ggatcgccac ccggccaaag ctttgcgcca tcacgatctg
     2041 ctagagtgag ggcgtgcaga cgggactgct cccgcgcttc ttcgagagcg tgacccggca
     2101 ggccttcggt gatctggcga tcgacgacgc gcccgccctc cgctacctga gcgatctcct
     2161 gacccgcttc gtccgcaccg aggcgctccg catgatccgg gagctgccgg gtcggcgcct
     2221 cgactcggtg accgactcgc tcctcgcgat ccagcacgtg tgggccgccg acacccccgg
     2281 cttcaacccg ggcaccgagc gcgtgctgcg acggcagatc ggcgactacg ccctgttcat
     2341 gaccgggatc ttccgcgatc acgtggatcg cctcgcggtc accggctact acgaggacga
     2401 gggccggcgc gcctaccgct tcgtggcgga gctggcgcgc ggcggggccg ggtccgacgc
     2461 ctcgctctat cgccgcctcg cggatcgctt cgagatgtac gccggcgcgc tcaactacgc
     2521 ccgcaaggtc tacttccgcg cggacgcggc gcccgtcgcc gcgtggccct ccggtccgtt
     2581 cttccgcgcc ctgctctccg aatgaccgcc gcggctccgt cggacgcggc ggccgcctgc
     2641 ctggccgtgc tcgaagccgc cctcggcgac gccacgccgc ccgccctgcc ggcgggccgc
     2701 gcggacgatc tcgacgaggc gctgcgcgcg ctggcccggc gccacggcgc cgccgcggtg
     2761 ccgctgctcg agcggctggc cgaggccgcg cccgacaagg agcaccggcg cgccgtgcgc
     2821 cgcgcgctct accggctcga gcaatcgggg cagcggccgg cccgggcgcc cgcggcgacc
     2881 ccggcgcgcc cggtggtcgc gcgcgaggcc gagcacccgg tgcgcgcctg gctctccggc
     2941 atcgacggca gcggctcgcg cgcggcgtgg atcctcttcg agggcgggct cgggggcggg
     3001 ctccggctct gctcgctgat cctgaacgac gaggccggca tcatggacgc ggcgggcggc
     3061 cccatcgccc gcaagcggct cgaggccgag ctgcgggggc tgcgggagag ccagaagctg
     3121 ccgtgggtgg aggtcgacgg cgcgcgcgcc tgtgcgctgg tcgggcaggc gatcgagctg
     3181 catgagcggc tggggacggt gccgccgccg gagttcgcgc gatggcgaaa gagtttttct
     3241 ggcagttcga ccgtttcgag cttgtcgggc agttcgaccg tttc
//
LOCUS       DATMCV010000125         5207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_539045, whole genome shotgun sequence.
ACCESSION   DATMCV010000125 DATMCV010000000
VERSION     DATMCV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539045"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VNU03_03050"
     CDS             complement(<1..393)
                     /locus_tag="VNU03_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73455.1"
                     /translation="MKRLALAATLVVLATLPLWAGGNFYINIGSQILLYAVFALGINI
                     LVGYAGLVTLGHAGLLGIAAYTAARLLNGGYGHIAAVTAALALTLAAAALFATLALRG
                     SGSGIGFVMITLAIGQIVWGVAYRWISIT"
     gene            complement(390..1250)
                     /locus_tag="VNU03_03055"
     CDS             complement(390..1250)
                     /locus_tag="VNU03_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73456.1"
                     /translation="MSLWLVLAVNSVTLGGLLFLLSAGFSLIFGLMRIPNLTHGSFFM
                     LGGYFAASLIEWGLGFWPAAMVSGLLVAALGGVVERLILRRLAGAELAQVLVTLGLSF
                     MIADVCLKVWTGDPVRIGTPPGLQGVTAVGSFAFPTYRLAISLVAVAFAVALWLLMDR
                     TRLGAMIRAGVDDPDMARVAGIRVSRLFTIVFCLGAWLAGFAGVIGGPILSVYPGLDQ
                     EMLPLALVVVILGGSGSLLGSLVGSFVVGFLYNFGQAMFPELAYVVLFLPMLLVLVVR
                     PQGLFGRPAV"
     gene            complement(1268..2506)
                     /locus_tag="VNU03_03060"
     CDS             complement(1268..2506)
                     /locus_tag="VNU03_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73457.1"
                     /translation="MSLTLSRRSFLAGSTILAAGTAGLPTRARAQSKPVRIGLLTVKT
                     GPLAAGGIQMEQGTLRFLKDRNNMMAGRKVELVVADTGGNPAGTKTKTQELVERENVD
                     MIFGPLAAFELLAITDYTAQAKVPVLSLAAAEDMTQRRPNPYFIRASATSSQNMHPLA
                     DYAAKELKFKTVITIADDFAFGHEQMAGFQRVFEDAGGRVVKKLWPPLTTPDYTPYLA
                     QISGADAVVQGFAGSNPLKFMKQYKDQGLKLPVLGGGPACDDALLKSYGDEAVGMISC
                     SAYTGDLDTPSNKRLIDGMVRDYGNIPGLYSAGLYINGQVAEAALEKTGGKTDDREAV
                     NRALRAVSLTDTPRGPFTFDRLGNVVGTFYIRRCERKGDKLVNTTIKTYPKVSQFWTY
                     DEKWFLAQPVYSRDYPPLKG"
     gene            complement(2530..3069)
                     /locus_tag="VNU03_03065"
     CDS             complement(2530..3069)
                     /locus_tag="VNU03_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP73458.1"
                     /translation="MARVLGLGHVGIYVRDLDRMAAFYRDVMGMRVTKQNRRAGVVFL
                     SADPESVDHEIALMGGRPDPADPHLINQISMRVGSLDDLRVMRRRLVAEGYRIEGVVN
                     HASAIGCYFFDPEGNRTEVFWVTGRPCWVPTATPIDIDQPDETVLAEVDRVWNQLRHV
                     PVGGRMTEESATVEVTRQP"
     gene            complement(3125..4231)
                     /locus_tag="VNU03_03070"
     CDS             complement(3125..4231)
                     /locus_tag="VNU03_03070"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP73459.1"
                     /translation="MLFTCPADAARGGGPRSRAGGRRPFCVDIHCHVHHPAADEMVKH
                     LMSPDREPSARFSNELSRATNRKQMENVWSCLTSVEQRLRDMDKMGMDVQAISPSPFH
                     FMYWLEPELARKVSRSVNEHLASIVKAHPDRFVALAHVPLQAPDLAAEELAYCVTQLG
                     FRGAEIGTNVVGAEVSRGRDRFWSRAQELDAVVFMHPNGFTQGERLTDHYFTNVIGNP
                     LDTTVALGHLVFDGVLERFPKLKLVAAHGGGYIAHYPARMDHVWGARVDARTVLKNTP
                     RRSLARVYFDTIVFDRTQLEHLVNLWGADHVLVGTDYPYDMGMYDPRGFVGGAALKPA
                     DRAKILGLNAARLLKIKAPGKAGSAKPGRARGRR"
     gene            4395..5126
                     /locus_tag="VNU03_03075"
     CDS             4395..5126
                     /locus_tag="VNU03_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWP73460.1"
                     /translation="MALALALLALTAWNGAEAAEITVLSTVGMQPATTELIAQFERES
                     GHKIAVTYGLAAVLKTRFLEGAPADVLILTSPIVEDLARQNKVVPGSKVDVARSGVGI
                     GVKAGAPRPDISTPDALKSTVLAAKSVGFSKEGASGVAFAKALERLGIAEQVRAKYKD
                     TGTKAGEMLVSGDIELGAAQVPELMAVPGVDVVGPLPADLQTVTVFSVGLATEAKAAD
                     AAKAFIQFLTGPAAAPVYKAKGLGG"
BASE COUNT          836 a         1760 c         1874 g          737 t
ORIGIN      
        1 cgtgatgctg atccagcgat acgcgacgcc ccagacgatc tggccgatcg ccagggtgat
       61 catgacgaag ccgatgccgc tgccgctgcc gcgcagcgcg agggtcgcga agagggcggc
      121 ggccgcgagg gtgagggcga gcgccgccgt gaccgcggcg atgtggccgt acccgccgtt
      181 caggaggcgg gccgcggtgt aggccgcgat gccgaggagc ccggcgtggc cgagcgtgac
      241 gagcccggcg tagcccacca ggatatttat gccgagcgcg aagacggcgt agaggaggat
      301 ctggctcccg atgttgatgt agaagttgcc gccggcccag aggggcagcg tggccaggac
      361 cacgagcgtg gcggccagcg cgagccgctt cacaccgccg gccgcccgaa gagaccctgg
      421 ggtcgcacca ccagcacgag cagcatggga aggaacagca ccacgtaggc cagctcgggg
      481 aacatcgcct ggccgaagtt gtagaggaag ccgacgacga agctgccgac cagcgagccc
      541 aggaggctgc cgctgccccc gaggatgacc accacgaggg ccagcgggag catctcctgg
      601 tcgaggccgg gatagaccga gaggatgggc ccgccgatga ccccggcgaa gccggccagc
      661 cacgcgccca ggcagaagac gatggtgaag aggcgcgaga cgcggatgcc cgcgacgcgc
      721 gccatgtccg ggtcgtcgac gccggcccgg atcattgcgc ccagccgggt ccgatccatc
      781 agcagccata gcgccaccgc gaaggccacc gcgacgaggc tgatggccag gcggtatgtc
      841 ggaaacgcga agctgccgac cgcggtcacg ccctgcagcc cgggcggcgt gccgatccgg
      901 acgggatcac cggtccagac cttgaggcac acgtccgcga tcatgaagga gagcccgagg
      961 gtcaccagga cctgcgcgag ctccgcgccg gccagccgac gcaggatgag ccgctccacg
     1021 acgcccccga gcgcggcgac cagcagcccg ctgaccatcg cggccggcca gaagccgagg
     1081 ccccactcga tcaggctcgc cgcgaagtag ccgcccagca tgaagaagga gccgtgcgtg
     1141 aggttcggga tccgcatcag cccgaagatc agcgagaagc cggcggacag caggaacagc
     1201 aggccgccga gcgtgacgct gttgacggcc agcacgagcc agaggctcat ggcagagcgg
     1261 acgtcgatca gcccttcagg ggcggatagt cacgcgagta gaccggctgg gccaggaacc
     1321 acttctcgtc gtaggtccag aactggctca ccttcgggta cgtcttgatc gtggtgttga
     1381 cgagcttgtc gcccttgcgc tcgcagcgcc ggatgtagaa ggtgcccacc acgttgccga
     1441 ggcggtcgaa ggtgaacggc ccgcgtgggg tgtcggtcag cgacaccgcg cggagcgccc
     1501 ggttcaccgc ctcccgatcg tcggtcttgc cgcccgtctt ctccagcgcg gcctccgcga
     1561 cctggccgtt gatgtagagg cccgcggagt agaggccggg aatgttgccg tagtcgcgga
     1621 ccatcccgtc gatcagccgc ttgttgctcg gggtatcgag gtcgccggtg taagccgagc
     1681 aggagatcat gccgaccgcc tcgtcgccgt agctcttgag cagcgcgtcg tcgcaggccg
     1741 gcccgccgcc caggacgggc agcttgagcc cctggtcctt gtactgcttc atgaacttga
     1801 gcgggttcga gccggcgaag ccctgcacca ccgcgtccgc gccgctgatc tgggccaggt
     1861 acggcgtgta gtcgggggtg gtcagcggcg gccacagctt cttcaccacc cggccgcccg
     1921 cgtcctcgaa gacgcgctgg aagccggcca tctgctcgtg gccgaacgcg aagtcgtcgg
     1981 cgatggtgat gacggtcttg aacttgagct ccttcgccgc gtagtcggcg agcgggtgca
     2041 tgttctggga agaggtggcg gaggcgcgga tgaagtacgg gttgggccgg cgctgcgtca
     2101 tgtcctccgc cgcggccagg ctcagcaccg gcaccttcgc ctgcgcggtg tagtcggtga
     2161 tggcgagcag ctcgaaggcg gccaggggcc cgaagatcat gtccacgttc tcgcgctcaa
     2221 ccagctcctg ggtcttggtc ttggtgccgg ccgggttgcc gccggtgtcg gccaccacga
     2281 gctcgacctt gcggccggcc atcatgttgt tgcgatcctt gaggaagcgc agcgtcccct
     2341 gctccatctg gatgccaccc gcggcgagcg gcccggtctt cacggtgagg aggccgatgc
     2401 gcacgggctt gctctgggcc cgggcccgcg tgggcaggcc cgcggtcccc gcggcgagaa
     2461 tcgtgctacc ggccaggaag gaacggcggc tgagtgtcag cgacattgct gcctccttgg
     2521 agaagtgggc tagggctggc gggtcacctc gacggtcgcg ctctcctcgg tcatccgccc
     2581 gcccaccggg acgtggcgga gctggttcca gacgcggtcg acctcggcga ggacggtctc
     2641 gtccggctgg tcgatatcga tgggggtggc ggtcgggacc cagcacggcc ggccggtgac
     2701 ccagaacacc tcggtgcgat tgccctccgg atcgaagaag tagcagccga tcgcgctcgc
     2761 gtgattgaca acgccctcga tgcggtagcc ctcggcgacg aggcgccggc gcatcacgcg
     2821 gaggtcgtcg agactgccga cccgcatcga gatctggttg atcaggtggg gatccgccgg
     2881 gtccgggcgg ccgcccatga gcgcgatctc gtggtccacc gactccggat ccgcgctcag
     2941 gaagacgacc ccggcgcgcc ggttctgctt ggtcacccgc atgcccatga cgtcccggta
     3001 gaacgcggcc atccgatcca gatcgcgcac gtagatcccg acgtggccta gccccagcac
     3061 tctcgccatg tgtcgcctcc cgcgtcagag ctcacccgcc gctccgacgg cccgaagaat
     3121 actcctatcg ccgaccgcgc gcccgcccgg gcttggccga tcccgccttc cccggcgcct
     3181 tgatcttgag cagccgggcc gcgttgaggc ccaggatctt cgcccggtcg gccggcttga
     3241 gggcggcgcc ccccacgaaa ccccgcgggt cgtacatccc catgtcgtag ggatagtcgg
     3301 tgccgacgag cacgtggtcg gcgccccaca ggttcacgag gtgctcgagc tgggtccggt
     3361 cgaagacgat cgtgtcgaag tagacgcggg ccagcgaccg gcgcggggtg ttcttcagca
     3421 ccgtccgcgc gtcgacccgc gcgccccaca cgtggtccat ccgagcgggg tagtgcgcga
     3481 tgtagccgcc cccatgggcg gcgaccagct tgagcttcgg gaaccgctcg agcacgccgt
     3541 cgaagacgag atggccgagc gcgacggtgg tgtcgagcgg gttgccgatg acgttggtga
     3601 agtagtggtc ggtcaggcgc tcgccctggg tgaagccgtt cgggtgcatg aagacgaccg
     3661 cgtcgagctc ctgggcccgg ctccagaacc ggtcccggcc ccgtgagacc tccgcgccga
     3721 ccacgttggt gccgatctcg gcaccgcgga acccgagctg ggtcacgcag tacgcgagct
     3781 cctcggccgc gaggtccggc gcctgcagcg gcacgtgggc cagcgcgacg aaccggtccg
     3841 ggtgcgcctt cacgatcgac gccaggtgct cgttcaccga gcggctcacc ttccgcgcca
     3901 gctccggctc gagccagtac atgaagtgga agggcgacgg cgagatggcc tggacgtcca
     3961 tgcccatctt gtccatgtcg cgcagccgtt gctcgacgga agtgaggcag gaccacacgt
     4021 tctccatctg cttgcggttg gtggcccggg agagctcgtt cgagaagcgg gcggacggct
     4081 cccggtccgg ggacatcaaa tgcttgacca tctcgtccgc ggcgggatgg tgcacgtggc
     4141 aatggatgtc gacgcagaac ggccggcggc cgccggcgcg ggagcgcggg cccccgcccc
     4201 gggccgcgtc ggcggggcac gtaaagagca tgggcgtctc ctcggtgtga gttgccggga
     4261 gtctaggcgg acgcgggcac cgtgtcaaag gcgcacccga cgggctatga tcgggccgga
     4321 cacggaccct ctcccctcag gggagagggc agggatcacg ggaggagctc atgaacaggc
     4381 gtgcgacccg cggtatggcc ctggcactcg cacttctcgc gctgacggcg tggaacggcg
     4441 ccgaggccgc cgagatcacg gtgctcagca ccgtcggcat gcagccggcc accaccgagc
     4501 tgatcgcgca gttcgagcgc gagagcggcc acaagatcgc ggtgacctac ggtctcgccg
     4561 cggtgctcaa gaccaggttc ctggagggcg cgccggcgga cgtcctcatc ctgaccagcc
     4621 cgatcgtcga ggacctggcc aggcagaaca aggtcgtgcc gggcagcaag gtggacgtcg
     4681 cccggtccgg cgtcggcatc ggggtgaagg cgggcgcgcc caggcccgac atcagcacgc
     4741 ccgacgccct caagagcacc gtgctggccg cgaagtcggt cggcttctcg aaggaaggcg
     4801 cctcgggcgt cgcgttcgcc aaggcgctcg agcgcctcgg catcgccgag caggtccgcg
     4861 ccaagtacaa ggacaccggc acgaaggccg gcgagatgct ggtctcgggc gacatcgagc
     4921 tgggcgccgc gcaggtcccc gagctgatgg cggtgcccgg cgtcgacgtg gtcggcccgc
     4981 tgccggcgga cctgcagacc gtcaccgtct tctcagtcgg cctcgccacc gaggcgaagg
     5041 cggccgacgc ggcgaaggcc ttcatccagt tcctgaccgg cccggcggcc gcgccggtct
     5101 acaaggccaa gggcctcggc ggctgagccg gctagaaggc gcggatgcgg ggcatcacgt
     5161 cacggccgat gcgctcgaac tagcgcagca tgagccggtg gtcaccg
//
LOCUS       DATMCV010000126         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_74036, whole genome shotgun sequence.
ACCESSION   DATMCV010000126 DATMCV010000000
VERSION     DATMCV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74036"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VNU03_03080"
     CDS             <1..301
                     /locus_tag="VNU03_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HWP73461.1"
                     /translation="EHIEQAEAKKAYEKAKVPMATLSERVRHVVQSSFDGRRIVIFSG
                     GATKENDEAIFDECRGIRDGGGFGSIIGRNSFQRPRDHALRFLDTVMKIYSGEKK"
     gene            318..1262
                     /locus_tag="VNU03_03085"
     CDS             318..1262
                     /locus_tag="VNU03_03085"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWP73462.1"
                     /translation="MPAPSESEVRGYLKTLSNWGRWGSEDELGTINLITPAKRQAAAR
                     LVSHGQSVTCARPITTDITPDTTVQPMRFMVDSGEGRDTVSPERALQRRGAAEFIGMV
                     FHGYTITHVDAPSHYFWEGQLYNGRSCNAVTSREGATVNSVEVLRDGVVSRGVLLDVA
                     RTRGVRWLEGGVGVMPEDLEAAERAAGVRVEAGDILLVRTGYYGRRLAEGPVHPLQAG
                     TPALHAACCPWLRERGVAMIGTDTHNDVHPAPYPSMGNSFHVVCLVAMGLWLIDNANL
                     EDLAQTAARLNRWEFLMTVAPLRLKNVTGSPVNPIAVF"
     gene            1344..1847
                     /locus_tag="VNU03_03090"
     CDS             1344..1847
                     /locus_tag="VNU03_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73463.1"
                     /translation="MERHTPWFQLVAIILVVIGLVTLAAPEPAHAMDPQLILAMASAA
                     GAIALIVGYLIVANGREKNKAASLEGVYACREREASGPMGCGGNASPDSIAVAVAPES
                     APHSPMAADPRFTARADALIAIAQSCAGGQASGPMGCGGPMGTEPRYSSASSSAPAFS
                     APAIQGQ"
BASE COUNT          298 a          755 c          718 g          293 t
ORIGIN      
        1 cgagcacatc gagcaggcgg aggccaagaa ggcctacgag aaggccaagg tcccgatggc
       61 gaccctcagc gagcgggtgc gccacgtggt gcagtcgtcc ttcgacgggc gccgcatcgt
      121 gatcttctcg ggcggggcga ccaaggagaa cgacgaggcg atcttcgacg agtgccgcgg
      181 catccgcgac gggggcggct tcggctcgat catcgggcgc aactcgttcc agcggccgcg
      241 cgatcacgcg ctccgattcc tggacaccgt gatgaagatc tactcgggcg agaagaagta
      301 gcggtccggc cgggctcgtg cccgctccct cggagtcaga agtccgcggc tacctgaaga
      361 ccctgtccaa ctggggccgc tgggggagcg aggacgagct cggcaccatc aacctgatca
      421 cccccgccaa gcgccaggcc gcggcccgcc tcgtgagcca cggccagagc gtgacctgcg
      481 cgcggccgat caccaccgac atcacccccg acaccaccgt ccagcccatg cgcttcatgg
      541 tggactccgg ggaagggcgc gacaccgtct ccccggagcg cgccctgcag cgccggggcg
      601 ccgccgagtt catcggcatg gtgttccacg gctacacgat cacccacgtc gacgcgccct
      661 cgcactactt ctgggagggc cagctctaca acgggcgctc gtgcaacgcg gtgacctcgc
      721 gcgagggcgc caccgtcaac tcggtcgagg tgctccgcga cggggtggtg agccggggcg
      781 tgctgctgga cgtcgcgcgg acccgcgggg tccgctggct cgagggcggg gtgggcgtga
      841 tgcccgagga cctggaggcc gccgagcggg cggccggcgt gcgggtcgag gcgggcgaca
      901 tcctgctcgt gcgcaccggc tactacgggc ggcggctcgc ggaggggccg gtgcacccgc
      961 tgcaggcggg gaccccggcg ctgcacgcgg cctgctgccc ctggctgcgg gagcgtgggg
     1021 tcgcgatgat cggcaccgac acccacaacg acgtccatcc cgcgccgtac ccgtcgatgg
     1081 gcaactcctt ccacgtggtc tgcctggtgg ccatggggct ctggctgatc gacaacgcca
     1141 acctggagga cctggcccag accgcggccc ggctcaaccg gtgggagttc ctgatgacgg
     1201 tggcccccct ccgtctgaag aacgtgacgg gctccccggt caatccgatc gcggtcttct
     1261 agcccgcctc ccgccggcgg tctcgccgcg gcgcggccat ttccttggcg gacggaaaat
     1321 ccgcctgaca tatactcggg aacatggagc gccacacccc ctggttccag ctcgtggcca
     1381 tcattctggt cgtcatcggc ctggtcaccc tggccgcccc ggagccggcc cacgcgatgg
     1441 atccccagct catcctggcg atggcctccg cggccggcgc catcgcgctg atcgtgggct
     1501 acctcatcgt ggccaacggg cgtgagaaga acaaggccgc ctccctcgag ggggtctacg
     1561 cgtgtcggga gcgagaggcc agcggcccga tgggctgcgg gggcaacgcg agccccgact
     1621 cgatcgcggt cgccgtggcg ccggagagcg ctccgcactc tccgatggct gctgatcccc
     1681 gcttcaccgc ccgcgccgac gccctgatcg cgatcgccca gtcctgcgcg ggcggccagg
     1741 cctccggtcc gatgggctgc ggcggcccga tgggcaccga gccccgctac tccagtgcct
     1801 cctcgtccgc tcccgccttc tccgcccccg ccatccaggg ccagtaactc ccccggaagg
     1861 ggtcaggtcc tacaacccga cattcggggt ctgtccggca gggtctgttc ggcgaggcct
     1921 gtccgggccc tgaccggaaa gagcgagccc ggcggggcga cccccgccgg gctcgtggtg
     1981 cggatgcggc gcgcggagct acttgcggac cgcctcgatg acctcgccga agagcgccca
     2041 cttctcgccg tcgaacttgg cgag
//
LOCUS       DATMCV010000127         4872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_6850, whole genome shotgun sequence.
ACCESSION   DATMCV010000127 DATMCV010000000
VERSION     DATMCV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6850"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /gene="nuoB"
                     /locus_tag="VNU03_03095"
     CDS             <1..324
                     /gene="nuoB"
                     /locus_tag="VNU03_03095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HWP73464.1"
                     /translation="GPVYDSERFGIHFVASPRHADLLLVTGPVTRNMEVPLRKTYEAT
                     PAPKLVVAVGDCARTCGVFQGSYAVVGSVDRVIPVDVFVDGCPPEPAQILRGILTALR
                     RPLPA"
     gene            329..2368
                     /locus_tag="VNU03_03100"
     CDS             329..2368
                     /locus_tag="VNU03_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWP73465.1"
                     /translation="MAALLLAVMLGAYGAGAAAALLGGGHRAGRALSTAGAMTGAAAG
                     LLLGLQVLAGGPVWTFAAPDVVGLAGGIALRMDALGAFFLALIGMVAAPAALYGVGYS
                     AGYAGRYSLRGLGAALNLFLLATSLVPLADNVVTFLLAWEGMSLTSYFLVLTDHEDPE
                     TVSAGNWYLGMTQVGLAFLLAAFLGLGAGAPSLDFVDLRAAAAGLSGSARGAVFALAL
                     VGFGSKAGLIPLHVWLPRAHPAAPSHVSALMSGVMIKLGIYGLLRVGLDLLGGGPPWW
                     GALLIGLGAGSAVLGVLYALMEDDVKRLLAYSSVENIGLITLGVGAGFLFQSLAMPGP
                     AALALAAALYHALNHAAFKGLLFLGAGSVLHATGTRNMNRHGGLIRRLPWTGPCFLLG
                     AVAISGLPPLNGFFSEWLLFQSLLPGIGVASPAVAVLMVLALGMLALTAGLAAAAFAK
                     AFGITFLALPRSDAAAAAHESPVSMRAGMMALAAACVVLAAGALPVLATAGVVAAGLL
                     GLSAEPPTFALWLSFRTPAGLARMSPPAVIVGLLGGVAIVWLVVRALASRRPLRFADT
                     WGCGRVGQSPRMEYTATAYAEPLRRVFSAIYRPTEDVTVDHHPESHYFVQAIAYRTSI
                     LPWFERYLYEPLLSGIARGGRRALALQSGSVHAYLAYVVGALVILLGVLMITGAP"
     gene            2365..3315
                     /locus_tag="VNU03_03105"
     CDS             2365..3315
                     /locus_tag="VNU03_03105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HWP73466.1"
                     /translation="MRAASGYALALGQALLCVALAPGLIGLIRWLKARMQNRRGAPVW
                     QPYLELRKQLAKETVVSTNASWLFRLAPYVVFTSTLGVVLLLPVLAVPLPLDAVGDLL
                     VVVYLLLLGTFFLALAGLDPGSPFGGMGSSREMTVAALAEPTIAMAMFALALGAGSTN
                     LGEIVARAMARPAEAVSPGYLLAFTALFIVTLAETGRLPVDNPATHLELTMIHEAMVL
                     EYSGRYLALIEWAAALKLLVFFTLMANLFVPWGVAVHLGAGALLLAALTYVAKLAALA
                     AVVAVFETRVAKLRLFRVPELLSVSFVLALLAITASFLSR"
     gene            3320..3976
                     /locus_tag="VNU03_03110"
     CDS             3320..3976
                     /locus_tag="VNU03_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73467.1"
                     /translation="MDGLFSQLAMLGSSLVLIASFLLLWRRGIPAYVDAFTLQSLALG
                     ATTAVVGYFTGNRELYLVAGLLIGIKGVGIPLLLRRMARRLGSGRGVPPYVNTATSLL
                     VGGLLVLLAYIVMRPVVLLSELPTRAGMPLAMGVVFISLFVVISRRHALTQVIGFLML
                     ENGIALLAVLGTYGIPLIVEIGVFLDVMMGFLVMQIFVYQIHETFQSLDVQQLNRLRR
                     "
     gene            3980..4723
                     /locus_tag="VNU03_03115"
     CDS             3980..4723
                     /locus_tag="VNU03_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWP73468.1"
                     /translation="MPEPVLFLVLLGAPLVAALLAVGGPAAPRPVAVLTGLSALGSLG
                     AALGLAARVVAGGVPTWGPGELFRVDALSAVLAVCVAFVSALALGLGPGGGHESPREV
                     RWFRAFASLFAFTMLVAVTTNNVAVMWVAIEATTITSALLVPLHVSRASVEASWKYIL
                     IGSVGIALAFVGTVLAYFDFVTVAGPQESALHWTVLMAAAPALHPAAMQLAFVFLLVG
                     YGTKAGLAPMHTWLPDAHAEAPAALSAMM"
BASE COUNT          469 a         1784 c         1759 g          860 t
ORIGIN      
        1 gggccggtct acgacagcga gcggttcggc atccacttcg tcgcctcgcc ccgccacgcc
       61 gatctgctcc tcgtcaccgg accggtcacg cgcaacatgg aggtgccgct ccgcaagacc
      121 tacgaggcca cccccgcgcc caagctggtg gttgcggtgg gtgactgcgc ccgcacgtgc
      181 ggggtcttcc agggcagcta cgcagtggtg gggagcgtcg accgggtcat cccggtggat
      241 gtgttcgtcg acggctgccc gcccgagccg gcccagatcc tccgtggcat cctgaccgcc
      301 ctgcgccggc ctctgcccgc ctagatccgt ggccgccctc ctgctcgcgg tgatgctcgg
      361 cgcctacggc gcgggcgccg ccgcggcgct cctgggcggc ggccatcgcg ccggccgcgc
      421 gctctcgacc gcgggcgcga tgaccggcgc ggcggccggg ctgctgctcg ggctgcaggt
      481 gcttgccggg ggcccggtct ggaccttcgc cgcgcccgac gtggtcgggc tcgcgggcgg
      541 catcgcgctg cggatggacg cgctcggcgc gttcttcctg gccctgatcg gcatggtcgc
      601 ggcgccggcg gccctctacg gcgtcggcta ctccgcgggg tacgcgggcc gctactcgct
      661 ccgcggcctc ggggccgcgc tgaacctgtt cctcctcgcc accagcctgg tgcccctcgc
      721 cgacaacgtg gtgaccttcc tcctcgcgtg ggagggcatg tcgctgacct cctatttcct
      781 ggtgctcacc gaccacgagg atccggagac ggtcagcgcc ggcaactggt acctcgggat
      841 gacccaggtc gggctcgcgt tcctgctcgc cgcgttcctg gggctcggcg cgggggcgcc
      901 ctcgctcgat ttcgtcgatc tccgggcggc cgccgccggc ctgagcggga gcgcgcgcgg
      961 cgcggtgttc gcgctcgcgc tggtcggctt cggctccaag gcggggctga ttccgctcca
     1021 cgtgtggctg ccgcgcgccc acccggcggc gccgagccac gtctcggccc tcatgtcggg
     1081 cgtgatgatc aagctcggca tctacgggct gctgcgggtc ggcctcgatc tcctcggcgg
     1141 ggggccgccg tggtggggcg cgctgctgat cgggctgggc gccggctccg cggtgctcgg
     1201 ggtgctctac gccctgatgg aggacgacgt caagcggctg ctggcgtact cgagcgtgga
     1261 gaacatcggc ctgatcacgc tcggggtggg cgcgggcttt ctcttccaga gcctggcgat
     1321 gccgggcccc gccgcgctgg ccctcgcggc cgcgctctat cacgcgctga accacgccgc
     1381 gttcaaggga ttgctgttcc tcggcgcggg ctccgtgctg cacgccaccg ggacgcgcaa
     1441 catgaaccgg cacggcgggc tcatccgccg cctgccgtgg accggcccgt gcttcctgct
     1501 cggcgcggtc gcgatctcgg gcctgccccc gctgaacggg ttcttcagtg agtggctgct
     1561 cttccagtcg ctcctgcccg gcatcggggt ggcctcgccg gcggtggcgg tgctgatggt
     1621 gctggccctc gggatgctgg ccctcaccgc gggcctcgcc gccgccgcct tcgccaaggc
     1681 cttcgggatc acgttcctcg cgctgccgcg ctcggacgcg gccgcggcgg cgcacgagtc
     1741 gccggtgtcg atgcgcgcgg gcatgatggc gctggccgcc gcgtgcgtcg tgctggccgc
     1801 gggcgccctg ccggtgctgg ccaccgccgg ggtcgtggcc gcggggctgc tcggtctctc
     1861 cgcggagccg ccgaccttcg cgctctggct ctcgttccgg acgcccgccg ggctcgcgcg
     1921 catgtcgcct cccgcggtga tcgtcgggct cctcgggggt gtcgcgatcg tgtggctggt
     1981 cgtccgcgcg ctcgcctccc ggcgcccgct gcgcttcgcg gacacgtggg gctgcggccg
     2041 ggtcggccag agcccgcgca tggagtacac cgcgaccgcc tacgcggagc cgctccggcg
     2101 ggtgttctcc gcgatctacc ggccgaccga ggacgtgacc gtcgatcacc atcccgagtc
     2161 gcactacttc gtgcaggcga tcgcctaccg caccagcatc ctgccgtggt tcgagcgcta
     2221 tctctacgag cccctgctga gcgggatcgc ccgcgggggc cgacgcgccc tcgcgctcca
     2281 gtcgggctcg gtgcacgcct acctggccta cgtggtcggc gcgctcgtga tcctgctcgg
     2341 ggtcttgatg atcaccggcg cgccgtgagg gcggcgagcg gctacgcgct cgcgctgggc
     2401 caggcgctcc tgtgcgtggc cctggccccc gggctcatcg gactgatccg gtggctgaag
     2461 gcgcggatgc agaaccggcg tggggcgccg gtctggcagc cgtacctcga gctgcgcaag
     2521 cagctggcca aggagacggt ggtatcgacc aacgcctcgt ggctcttccg gctcgcgccc
     2581 tacgtggtct tcaccagcac cctcggcgtc gtgctgctgc tcccggtcct cgcggtgccg
     2641 ctgccgctcg acgcggtcgg cgacctcctg gtggtggtct acctgctgct gctcggcacc
     2701 ttcttcctgg ccctggccgg cctcgatccg ggctctccgt tcgggggcat ggggtcgagc
     2761 cgcgagatga cggtggccgc gctcgccgag cccaccatcg cgatggcgat gttcgcgctg
     2821 gcgctgggcg cgggctccac caacctgggc gagatcgtgg cccgggcgat ggcgcgaccc
     2881 gcggaggcgg tgagcccagg ctacctgctc gccttcaccg cgctcttcat cgtgacgctg
     2941 gccgagaccg ggcggctgcc agtggacaac ccggcgacgc atctcgagct gaccatgatc
     3001 cacgaggcta tggtcctcga gtactcgggc cggtacctgg ccctcatcga gtgggccgcc
     3061 gcgctcaagc tgctggtctt cttcaccctg atggccaacc tcttcgtgcc gtggggtgtg
     3121 gcggtccacc tgggcgcggg cgcgctcctg ctcgccgcgc tgacctacgt ggcgaagctc
     3181 gcggcgctcg ccgcggtggt ggcggtgttc gagacgcggg tggccaagct ccggctcttc
     3241 cgggtaccgg agctgctgag cgtctcgttc gtcctggcgc tgctcgctat caccgcctcg
     3301 ttcctgtcga ggtagcctga tggacggcct cttctcccag ctcgccatgc tcggctcgag
     3361 cttggtgctc atcgcctcgt tcctgctcct gtggcgccgc gggatcccgg cctacgtcga
     3421 cgcgttcacg ctccagtcgc tggccctcgg ggcgaccacc gcggtcgtcg gctacttcac
     3481 gggcaaccgc gagctctacc tggtggccgg cctcctgatc gggatcaagg gggtggggat
     3541 cccgctgctg ctgcgccgga tggcccgccg cctcgggagc gggcgcgggg tgccgcccta
     3601 cgtgaacacc gccacgtcgc tgctggtggg cgggctgctc gtgctgctcg cctacatcgt
     3661 gatgcggccg gtggtcctgc tgagcgagct gccgacgcgg gcgggcatgc cgctggcgat
     3721 gggtgtggtc ttcatcagcc tcttcgtggt catcagccgc cggcacgcgc tcacccaggt
     3781 gatcggcttc ctgatgctcg agaacggcat cgccctgctc gcggtgctcg gcacctacgg
     3841 catcccgctg atcgtcgaga tcggcgtgtt cctcgacgtg atgatgggct tcctcgtcat
     3901 gcagatcttc gtgtaccaga tccacgagac cttccagagc ctggacgtcc agcagctcaa
     3961 ccggttgagg cgctgacgga tgcccgagcc cgtcctgttc ctggtcctgc tcggcgcgcc
     4021 gctggtcgcg gccctgctcg cggtcggcgg ccccgccgcg ccgcggccgg tcgcggtcct
     4081 gaccgggctg tcggcgctcg gctcgctcgg ggcggccctc gggctggccg cccgcgtggt
     4141 ggcgggaggg gtgcctacct gggggcccgg agagctcttc cgggtggacg cgctctcggc
     4201 ggtgctcgcg gtctgcgtgg ccttcgtgtc ggcgctcgcg ctcgggctcg ggcccggcgg
     4261 aggccacgag agcccgcgcg aggtgcggtg gttccgggcc ttcgcgagcc tcttcgcctt
     4321 cacgatgctg gtggcggtca ccaccaacaa cgtggcggtg atgtgggtgg ccatcgaggc
     4381 gaccacgatc acctccgcgc tgctggtccc gctccacgtg agccgggcct cggtggaggc
     4441 ctcctggaag tacatcctga tcggctcggt gggcatcgcg ctcgccttcg tgggcacggt
     4501 cctcgcctac ttcgacttcg tcacggtggc cgggccgcag gagtcggcgc tccactggac
     4561 ggtgctcatg gccgcggcgc cggcgctcca ccccgccgcg atgcagctcg ccttcgtgtt
     4621 cctgctggtg ggctacggga ccaaggcggg cctggccccg atgcacacct ggctgcccga
     4681 tgcccacgcg gaagcgccgg ccgcgctctc ggccatgatg tagggcgtgc tgctcgcggt
     4741 cgcgctctac gcgatcgtgc gctggaaggc ggtggtcaac ctcgcggtgg acgggccgct
     4801 gcccgacggc ctcctcatcg ggctcgggct tctctcggtc gccatcgcgg cgctctcgct
     4861 gctggggcag cg
//
LOCUS       DATMCV010000128         3315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_606848, whole genome shotgun sequence.
ACCESSION   DATMCV010000128 DATMCV010000000
VERSION     DATMCV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606848"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VNU03_03120"
     CDS             <1..609
                     /locus_tag="VNU03_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWP73469.1"
                     /translation="DLVIPDQFYDHTRRRVSSFFGGGIVGHVGMADPVCPGLGDLLEA
                     AGREAGARVHRGGTYICIEGPQFSTKGESQIYRRWGVSVIGMTNMPEAKLAREAELCY
                     ATLALATDYDVWHESHAAVTVEAVVANLLKNVATAKVVLRRMIPRVGKACAAGCGDAL
                     ASAVITDPKRFPPRTRKSLALLLDRYFPTAPRPAAPRRGRRG"
     gene            602..1594
                     /locus_tag="VNU03_03125"
     CDS             602..1594
                     /locus_tag="VNU03_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HWP73470.1"
                     /translation="MASGQLDVIGIGNAMVDVLTQADEDLVVRQGLAKGTMRLVDEAQ
                     ARALYEAMGPGVEMSGGSAANTVVGVASFGGRAHYVGKVRDDQLGDVFGHDLRATGVG
                     YDTPRATSGPPTGRCLILVTPDAQRTMSTFLGASVRLGPADVDRGLIARGKILYLEGY
                     LFDPPDAQEAFRAAAAIAHAAGRKVALTLSDPFCVERHRAAFLDLVQHHVDILFANEA
                     EICALYQVRDFDAAVSHVRGHCELAALTRSAKGSVLVQGDRLHVVQAHPVDAVVDTTG
                     AGDLYASGVLYGLAQGLDLPTCGRLGSLAAAEVIAHVGARPMVPLTELAAAVLG"
     gene            1600..2433
                     /gene="larE"
                     /locus_tag="VNU03_03130"
     CDS             1600..2433
                     /gene="larE"
                     /locus_tag="VNU03_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014097026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HWP73471.1"
                     /translation="MADVGALAPKYDRLLELLRGMTRVIVAFSGGVDSTFLARAAKDA
                     LGGRALLVTADSETYPTAELEETRRLAGLLGMPHEVIQTRELDRPEYARNGPDRCFHC
                     KEELFSRLEPIARRAGDAQMVYGANMDDLGDHRPGMKAAEARGVRAPLIEADLWKEEI
                     RALSRDLGLPTWDKPSFACLSSRFQYGDAITAAKLRQIDEAEAFVRSLGFRQFRVRHH
                     DRLARIELPAEEMPRLWQEDRHGAIVRRFRELGYLYVTLDLQGFRSGSANEALRWIGK
                     K"
     gene            2415..3173
                     /gene="larB"
                     /locus_tag="VNU03_03135"
     CDS             2415..3173
                     /gene="larB"
                     /locus_tag="VNU03_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWP73472.1"
                     /translation="MDREKVRALLEEVAAGALAPEQALARLRTLPYDDLRFARVDLHR
                     SLRHGVPEAVYCAGKTPEQVVAIVERLAQHHDNVLATRAEPAVVAALVASGLPCRVHE
                     AARLVVVKATARDGVGLIVVVSAGTSDLPVAEEAAVTAEVMGNHVERLFDCGVAGLHR
                     LVPHLDRLNEANAIVAVAGMDGALPSVLGGLVDRPVIAVPTSVGYGASFGGVAALLAM
                     LNSCAPGVSVVNIDNGYGAAAQANQINKLATKLR"
     gene            3242..>3315
                     /gene="larC"
                     /locus_tag="VNU03_03140"
     CDS             3242..>3315
                     /gene="larC"
                     /locus_tag="VNU03_03140"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HWP73473.1"
                     /translation="MKVAYFDCPSGASGDMILGALVDAG"
BASE COUNT          449 a         1197 c         1212 g          457 t
ORIGIN      
        1 gatctcgtca tcccggacca gttctacgac cacacccgcc ggcgcgtctc gagcttcttc
       61 ggaggcggca tcgtggggca cgtgggaatg gccgacccgg tgtgccccgg tctcggcgac
      121 ctgctcgagg ccgccgggcg cgaggcggga gcgcgggtgc atcgcggggg gacctacatc
      181 tgcatcgagg gcccgcagtt ctccaccaag ggggaatcgc agatctaccg ccgctggggc
      241 gtctcggtca tcggcatgac caacatgccc gaggccaagc tcgcccgcga ggccgagctc
      301 tgctacgcga cgctcgcgct ggccaccgac tacgacgtgt ggcacgagag ccacgccgcg
      361 gtgacggtcg aggcggtggt ggcaaatttg ttgaagaacg tggcgaccgc caaggtggtc
      421 ctgcggcgga tgatcccgcg ggtggggaaa gcctgcgcgg ccggctgcgg cgacgcgctg
      481 gcgagcgcgg tcatcaccga cccgaagcgc tttccgccgc gtacgcgcaa gagcctggcc
      541 ctgctcctcg atcgctattt cccgacggcg ccccgcccgg ccgccccgcg gaggggtcgc
      601 cgtggctagc ggccagctcg acgtgatcgg catcggcaac gcgatggtgg acgtgctgac
      661 ccaggccgac gaagatctgg tcgtccggca gggcctcgcc aagggcacga tgcgcctggt
      721 cgacgaggcc caggcccgcg cgctctacga ggcgatgggc ccgggggtgg agatgtctgg
      781 cggctccgcg gccaacaccg tggtgggcgt cgcctccttt gggggccggg cgcactacgt
      841 gggcaaggtc cgcgacgatc agctcggcga cgtgttcggg cacgacctgc gcgcgaccgg
      901 cgtcggctac gacaccccgc gggcaaccag cgggccgccc accgggcggt gcctcatcct
      961 ggtcaccccg gacgcgcagc gcacgatgag cacgttcctc ggcgcctcgg tgcggctcgg
     1021 tcccgccgac gtggaccgcg ggctgatcgc gcgggggaag atcctctatc tcgagggcta
     1081 cctcttcgac ccgccggacg cgcaggaggc gttccgcgcc gccgccgcca tcgcccatgc
     1141 ggccgggcgc aaggtcgcgc tcacgctgtc cgacccgttc tgcgtcgagc gccaccgcgc
     1201 cgccttcctc gacctggtcc agcaccacgt cgacatcctc ttcgcgaacg aggccgagat
     1261 ttgcgcgctc taccaggtgc gcgacttcga cgcggcggtc tcgcacgtgc gcgggcactg
     1321 cgagctggcc gccctgaccc ggagcgccaa gggctcggtg ctggtgcagg gggatcggct
     1381 gcacgtggtc caggcgcatc cggtcgacgc ggtggtggac accaccggcg cgggagacct
     1441 ctacgcctcc ggcgtgctct acgggctcgc ccaggggctc gatctgccga cgtgcgggcg
     1501 gctcggctcc ctcgccgccg ccgaggtcat cgcccacgtg ggcgcgcggc cgatggtgcc
     1561 gctgaccgag ctggccgccg ccgtgctggg atgaccggcg tggccgacgt cggcgccctc
     1621 gcgcccaagt acgaccggct cctggagctg ctccggggga tgacccgcgt gatcgtggcc
     1681 ttctccggtg gcgtggactc gaccttcctg gcccgcgcgg ccaaggacgc gctcggcggc
     1741 cgcgcgctgc tggtcaccgc cgactcggag acctatccga ccgccgagct cgaggagacc
     1801 cgccgcctcg ccggactgct cgggatgcct cacgaggtga tccagacgcg cgagctggac
     1861 cggcccgagt acgcgcgcaa cgggcccgat cgctgcttcc actgcaagga ggagctgttc
     1921 agccggctcg agcccatcgc ccggcgcgcg ggcgacgccc agatggtcta cggcgccaac
     1981 atggacgacc tgggcgacca ccggcccggc atgaaggccg ccgaggcgcg cggggtgcgc
     2041 gcgcccctga tcgaggccga cctgtggaag gaggagatcc gggcgctctc ccgcgacctg
     2101 gggctcccca cctgggacaa gccgtcgttc gcgtgcctct cctcgcgctt ccagtacggc
     2161 gacgcgatca ccgccgcgaa gctacggcag atcgacgagg cggaggcctt cgtgcggagc
     2221 ctgggcttcc gccagttccg ggtgcgccac cacgaccggc tcgcccgcat cgagctgccc
     2281 gcggaggaga tgccgcggct ctggcaggag gaccggcacg gcgcgatcgt ccgccgcttc
     2341 cgcgagctgg gctacctcta cgtgacgctc gacctgcagg gcttccgcag cggcagcgcc
     2401 aacgaggccc tccgatggat cgggaaaaag taagggccct gctggaggag gtcgcggccg
     2461 gcgcgctcgc ccccgagcag gcgctcgccc ggctccgcac gctgccgtac gacgacctcc
     2521 gtttcgcccg cgtcgacctg catcgctccc tgcgccacgg cgtgccggag gccgtctatt
     2581 gcgcgggcaa gaccccggag caggtggtgg ccatcgtgga gcgcctggcc cagcaccacg
     2641 acaacgtgct ggccacccgc gcggagcccg cggtggtggc ggccctggtc gcgagcgggc
     2701 tgccatgccg ggtgcacgag gccgcgcgcc tggtggtggt gaaggccacc gcgcgcgacg
     2761 gggtggggct catcgtggtg gtcagcgcgg gcacctccga tctcccggtg gccgaggagg
     2821 ccgcggtgac cgcggaggtg atgggcaacc acgtcgagcg gctgttcgat tgcggggtgg
     2881 cggggctgca ccgcctggtg ccgcacctcg accggctcaa cgaggccaac gcgatcgtgg
     2941 cggtggcggg catggacggc gcgctgccga gcgtgctggg aggcctcgtt gaccggccgg
     3001 tcatcgcggt gcccaccagc gtgggctacg gcgcctcctt cggcggcgtg gccgccctgc
     3061 tcgccatgct gaactcctgc gcccccggcg tctcggtggt caatatcgac aacggctacg
     3121 gcgcggccgc ccaggccaac cagatcaaca agctcgccac gaagctccgc tgacccccca
     3181 aaatgacgag cagtcgaccg cggagagggg gagtgcgggg gccatttctg ggcccgcgca
     3241 gatgaaagtc gcgtacttcg actgcccaag cggcgcctcc ggcgacatga tcctgggcgc
     3301 gctggtcgac gcggg
//
LOCUS       DATMCV010000129         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_670475, whole genome shotgun sequence.
ACCESSION   DATMCV010000129 DATMCV010000000
VERSION     DATMCV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670475"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2009
                     /locus_tag="VNU03_03145"
     CDS             <1..>2009
                     /locus_tag="VNU03_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP73474.1"
                     /translation="KARAGLESVGLPGDAADRYPAQFSGGQRQRIAIARALLRDAPIL
                     ILDEALSSVDAESEAAIQEALDRLMRGRTTLIFAHRLSSIVGADRILVLDDGAVAESG
                     THATLMARDGVYRRLMAGQAGGGRRDEGLFDDAPAGGADDGAAPASGDSSSAGSEDAP
                     AVLRATGPGWREVGRILFGLASGYHARLVITFVLGVARVAALIGVGLLSALVVRAVSR
                     GAPTRALLVALLIVAPAAGVLHWLESWLAHDMAYRLLADMRMRFFRKLVALGPAYLSA
                     RRTGDLLGVATHDIELIEYFFAHTITPGLVAVLVPAVVLGTLAVFGWPMAAAVLPFLA
                     YAALSPVLGRARIDRLSGRAREASGELNAHAVDSVQGLGEIVAFQQEARRGEALAARA
                     ASYAAARMPFLADLARQTALQDVATGLGGLAVTGVGAWLVASGRLESAMLPLLTLLAM
                     SAFVPVWEIAQVGRQLADTLAATRRVHAVHAEPVRIADGPGVAIPGPRRDDAAAIALH
                     DVTFTYPGRRRPALSSVSFTVPAGATVALVGPSGAGKTTIASLLLRFWDPDQGAITLA
                     SHDLRAWALEDLRRHIALVAQDTHLFNDTLGGNIRIARPDAAPADVAAAIERAALGPL
                     VTGLPEGLDTKVGERGLQLSGGQRQRVAIARAFLRDAPVLILDEA"
BASE COUNT          210 a          759 c          772 g          268 t
ORIGIN      
        1 gaaggcgcgc gcgggcctgg agtcggtcgg gctgcccggc gatgcggccg accggtaccc
       61 ggcccagttc tccggcggcc agcgccagcg catcgcgatc gcccgcgcgc tcctgcgcga
      121 cgcgccgatc ctgatcctgg acgaggcgct ctcatcggtc gacgcggaga gcgaggcggc
      181 gatccaggag gcgctcgacc gcctcatgcg cgggcgcacc accctgatct tcgcccaccg
      241 gctctcgagc atcgtcgggg ccgaccgcat cctggtcctg gacgacggtg cggtcgcgga
      301 gagcggcacc cacgcgaccc tcatggcgcg cgacggcgtc taccggcggc tcatggccgg
      361 gcaggccggc ggcggccgcc gcgacgaggg gctcttcgac gacgcgcccg ccggcggggc
      421 ggacgacggc gcggcgcccg cgagcggcga ttcctcgagc gcgggctccg aggacgcgcc
      481 cgcggtgctg cgcgcgacgg ggccgggctg gcgggaggtg ggccgcatcc tcttcgggct
      541 ggcgtccggc tatcacgccc gcctcgtcat caccttcgtg ctgggggtgg cgcgcgtcgc
      601 cgcgctgatc ggggtcgggc tcctgagcgc gctcgtggtg cgcgcggtga gccgcggcgc
      661 gccgacccgc gcgctgctgg tggcgctgct gatcgtggcc ccggccgccg gcgtgctcca
      721 ctggctcgag tcctggctcg cgcacgacat ggcctatcgc ctgctcgcgg acatgcgcat
      781 gcgcttcttc cgcaagctcg tggcgctcgg gccggcctac ctgtccgcgc gacgcaccgg
      841 cgatctgctc ggggtggcca cgcacgacat cgagctgatc gagtacttct tcgcccacac
      901 catcaccccg ggcctggtcg cggtgctggt gcccgcggtg gtgctcggca ccctcgccgt
      961 cttcggctgg ccgatggcgg cggcggtgct gccgttcctg gcctacgcgg cgctgagccc
     1021 ggtgctgggc cgcgcgcgca tcgaccgcct gagcgggcgc gcccgcgaag cgtccggtga
     1081 gctgaacgcg cacgcggtcg actcggtgca gggcctgggc gagatcgtgg cgttccagca
     1141 ggaggcgcgc cgcggcgagg cgctcgccgc gcgcgcggca tcctacgcgg cggcgcggat
     1201 gcccttcctc gcggatctcg cgcggcagac cgcgctgcag gacgtggcca ccggcctggg
     1261 cggcctcgcg gtgaccggcg tgggcgcgtg gctggtggcc tcgggtcggc tcgagagcgc
     1321 gatgctgccg ctcctgaccc tgctcgcgat gtccgcattc gtgccggtgt gggagatcgc
     1381 gcaggtgggc cgccagctcg ccgacaccct cgcggccacc cggcgggtgc acgcggtgca
     1441 cgccgagccg gtgcggatcg cggacgggcc gggggtggcg atcccggggc cgcgacgcga
     1501 cgacgcggcc gcgatcgcgc tgcacgacgt caccttcacc tacccgggac ggcgacggcc
     1561 cgcgctcagc tccgtgtcgt tcacggtgcc ggcgggcgcg accgtcgcgc tggtcgggcc
     1621 gtccggggcg ggcaagacca cgatcgcgag cctgctcctg cgcttctggg atccggacca
     1681 gggcgccatc acgctcgcct cacacgacct gcgcgcgtgg gcgctcgagg acctgcgccg
     1741 ccacatcgcg ctggtggccc aggacaccca cctgttcaac gacacgctcg gcggcaacat
     1801 ccgcatcgcg cggcccgacg cggcgcccgc ggacgtggcc gccgcgatcg agcgcgccgc
     1861 cctcggcccc ctggtgaccg ggctccccga gggcctcgac acgaaggtgg gcgagcgggg
     1921 gctgcagctc tcgggcgggc agcgccagcg cgtggccatc gcgcgcgcgt ttctgcggga
     1981 cgccccggtc ctgatcctgg acgaggcca
//
LOCUS       DATMCV010000130         3064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_606913, whole genome shotgun sequence.
ACCESSION   DATMCV010000130 DATMCV010000000
VERSION     DATMCV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606913"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1416)
                     /locus_tag="VNU03_03150"
     CDS             complement(127..1416)
                     /locus_tag="VNU03_03150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73475.1"
                     /translation="MKATRGGLHHAWVVLGALVVVMLLGSGLRAVFGVFIKPMEATFG
                     WDRAALSGAAAISLLLLGAAGPIVGWLADQWGPRRVIFVSAILLGLGAMLSSRVSALW
                     QIYITCGVLMGLGAGGVGMSTGAALAARWFEARRGLVMGLVGGAMSAGQLVIVPLAVW
                     FTLSYGWRQSFLYLGVLLLVIALPLTLLFIRDDPAQKGLKAYGAGQAARAGEPKAPPE
                     GRTRVADAMQVPAFWLLAGTFFICGYTSNGLVLTHLVPHAAEHGFSEMHAAQALGVMG
                     AMNILGTVASGFICDRFGRKGPLAFYYGVRGLSLLFLLYVWNVPSLHIFAAIFGLNYI
                     STVPPTTTLTANIFGRYSVGSLSGWIFFAHQVGAALGAAVGGWVFQSTGSYSWAFISA
                     ALLAFLAVPMALAIKEAPVTRTPQPTPAPAGLPATAS"
     gene            1537..1947
                     /locus_tag="VNU03_03155"
     CDS             1537..1947
                     /locus_tag="VNU03_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73476.1"
                     /translation="MATRRVLIVDDEAEVRSALTDILRAMRYTDTLEIEAVADGQAGL
                     DAVVRRLPDLILLDLQMPRMSGLTLLKQIRDVESRLPIIVISATDDSKVAAEALRGGA
                     VAYIPKPFDARHVEMLVATFFDSLRRREAKPSAS"
     gene            2144..2521
                     /locus_tag="VNU03_03160"
     CDS             2144..2521
                     /locus_tag="VNU03_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73477.1"
                     /translation="MSTKRVLVVDDQTPVRELVAAVLTADGHVVETARDGADALRVLD
                     GRGAFDLIVSDLKMPGLDGPSLYLELTRRWPDTRPHLLFISGFVESPEYAGFLQGASV
                     QVLLKPFDVDDLSRAVTAILAGA"
     gene            complement(2540..>3064)
                     /locus_tag="VNU03_03165"
     CDS             complement(2540..>3064)
                     /locus_tag="VNU03_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP73478.1"
                     /translation="AHPDVFNVLLQLLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHFF
                     REEKDPARVRGLITDLLKQALRPEFLNRIDETVIFQPLGRKEIASIVEIQARYLVKRL
                     ADRRITLELTPGARELLAREGYDPVYGARPLKRTIQRMIQDPLALELLNGTFGEGDTV
                     VVDAKGDTITFRKR"
BASE COUNT          506 a         1067 c         1079 g          412 t
ORIGIN      
        1 agaacttgat caggagcccg agactcaggc ggctctcgaa cgggaagtcc ttctgcatgc
       61 cggccagcga cgtcgtcgga ttgttcatgc ccatcattct acccctcgtg cccagccctc
      121 gacgagctag ctcgcggtcg cgggcagccc ggcgggggcc ggggtaggct ggggtgtccg
      181 ggtgaccggt gcctccttga tggccagggc catgggcacc gccaaaaacg cgagtagcgc
      241 cgccgagatg aaggcccacg agtaggagcc ggtggactgg aagacccagc cgcccaccgc
      301 cgcgccgagc gcggcgccga cctggtgcgc gaagaagatc cagcccgaga gcgaccccac
      361 cgagtagcgc ccgaagatgt tggcggtcag cgtggtggtg ggcggcacgg tcgagatgta
      421 gttgaggccg aagatggccg cgaagatgtg cagcgacggc acgttccaca cgtagagcag
      481 gaacagcagc gagaggcccc gcacgccgta gtagaaggcg agcggtccct tgcgcccgaa
      541 gcggtcgcag atgaagccgg aggccaccgt gcccaggatg ttcatcgcgc ccatcacgcc
      601 cagagcctgc gcggcgtgca tctcggagaa tccgtgctcc gccgcgtgcg ggaccaggtg
      661 cgtcagcacg aggccgttcg acgtgtagcc gcagatgaaa aaggtgcccg cgagcagcca
      721 gaacgccggc acctgcatgg cgtccgcgac ccgggtgcgg ccctccggcg gcgccttggg
      781 ctcgccggcc cgcgcggcct ggccggcgcc gtacgccttg agccccttct gggccggatc
      841 gtcccggatg aagagcaggg tgagcggcag cgcgatcacg aggagcagga cgcccaggta
      901 gaggaacgac tggcgccaac cgtagctgag cgtgaaccac accgcgagcg gcacgatcac
      961 gagctgtccg gccgacatcg cgccgcccac caggcccatc acgagcccgc gccgggcctc
     1021 gaaccagcgc gcggccaggg ccgcgccggt ggacatgccc accccgcccg cgcccagccc
     1081 catcagcacg ccgcaggtga tgtagatctg ccacagggcc gacacccgcg aggagagcat
     1141 cgcgcccagg ccgagcagga tcgcggagac gaagatgacg cgccgcgggc cccactggtc
     1201 ggcgagccag cccacgatcg gccccgccgc gcccagcagc agcagggaga tggccgcggc
     1261 gccggagagc gcggcccggt cccagccgaa ggtggcctcc atcggcttga tgaacacgcc
     1321 gaagacggcc cggaggccgg agcccagcag catgaccacc accagcgcgc ccaggacgac
     1381 ccaggcgtgg tgcaatccac cccgcgttgc cttcatgcat cctccccgga agagaaaaac
     1441 ccgcccagac catgccggcg aaccttgaga tggcaaaccg gcccccgcgg attccgcggg
     1501 cagggtggta gagtgtcgtc cgagaggacc cgggccatgg cgacgcggcg cgtgctgatc
     1561 gtggacgacg aggcggaggt gcgcagcgcg ctcacggaca tccttcgtgc gatgcgctac
     1621 accgatacct tggagatcga agcggtggcc gacggccagg ccggcctcga cgcggtcgtg
     1681 cgtcgcctgc ccgacctgat cctgctcgat ctgcagatgc cgcggatgag cgggctcacc
     1741 ctgctcaagc agatccgcga cgtggaatcg cgcctgccca tcatcgtgat ctccgccacc
     1801 gacgacagca aggtcgcggc ggaggccctg cgaggcggtg cggtggccta cattcccaag
     1861 ccgttcgacg cgcgccacgt cgagatgctc gtcgccacgt tcttcgactc gctccgtcgc
     1921 cgcgaggcga agccgagcgc ttcgtagcga aactgcaacg ttcgcgtgcg cacgatctgc
     1981 gtgcgcggag cgccagattc gtatcgacgg agggccgacg tccgtcctgt ccggcaagcg
     2041 acggcgcgcg cgcgccgaac tcgccaatcc cgcccaaatc cgcccgaccg cgagtcgcct
     2101 cgatctggaa cgggccttgc tccgtcattc cccggcggag cccatgtcga ccaagcgagt
     2161 gctcgtggtg gatgaccaga cgccggtgcg ggagctggtg gcggccgtgc tcaccgcgga
     2221 cggccacgtg gtggagaccg cgcgcgacgg agcggacgcg ctgcgcgtgc tcgacgggcg
     2281 cggggcgttc gacctcatcg tcagcgacct gaagatgccg gggctcgacg ggccgagcct
     2341 ctatctcgag ctgacccgcc gctggcccga cacgcgcccg cacttgctct tcatctccgg
     2401 cttcgtggag tcgcccgagt acgcgggctt cctgcagggg gcgagcgtgc aggtgctgct
     2461 caagccgttc gacgtcgacg acctgtcccg cgcggtcacc gcgatcctgg ccggcgcctg
     2521 aggcgccggc cagggccggt cagcgcttgc ggaaggtgat cgtgtcgccc ttggcgtcga
     2581 cgacgaccgt gtcgccctcg ccgaaggtcc cgttgagcag ctcgagggcg agcggatcct
     2641 ggatcatgcg ctggatggtc cgcttgagcg ggcgcgcccc gtacaccggg tcgtagccct
     2701 cgcgagccag cagctcccgg gcccccgggg tcagctccag ggtgatgcgc cggtcggcca
     2761 ggcgcttcac caggtagcgg gcctggatct ccacgatcga ggcgatctcc ttccggccga
     2821 gcggctggaa gatcaccgtc tcgtcgatcc ggttcaggaa ctccgggcgc agggcctgct
     2881 tgagcagatc cgtgatgagc ccgcgcaccc gcgccgggtc cttctcctcg cggaagaagt
     2941 ggctcccgag gttggaggtc atgatgacca ccgtgttacg gaagtccacc gtgcggccct
     3001 ggccgtcggt gagacggccg tcgtcgagga gctgcaggag cacgttgaag acgtcggggt
     3061 gggc
//
LOCUS       DATMCV010000131         3516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_607049, whole genome shotgun sequence.
ACCESSION   DATMCV010000131 DATMCV010000000
VERSION     DATMCV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607049"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VNU03_03170"
     CDS             complement(<1..748)
                     /locus_tag="VNU03_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP73479.1"
                     /translation="MKNPETKDTTLSQLDRRTFIKTAGVAAAAMGGIEGILAARRAPA
                     FAQGTKLHWVRWVDFIPESDVELKRQMPEASKALGAEVTFETINANDLQPRITAAIQS
                     GAGADIFNFQYNWAHLYQNAVVDVSDVAGDIGKAEGGFYEIYPPSCQVGGKWLAVPHS
                     IVGNAVAHRKSWLKEAGANEYPKTWDEARKVFAVLKKKGKPYGQTLGHTFGDAPTFTY
                     TMLWAFGGAETDKSGKKVVLDSKGALDSVKF"
     gene            complement(1073..2290)
                     /locus_tag="VNU03_03175"
     CDS             complement(1073..2290)
                     /locus_tag="VNU03_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73480.1"
                     /translation="MTNRWAVLALIMAAQTMANVGPLGIPSIAPLIRESLGLSVTQAG
                     SFLSAYYIGPVLMSLPAGWLADRWGVRGAMILGQALIAVGLGAVAVAPSFSFIVAILV
                     VAGAGYGVLNPTTTKAGMAWFPPRQRATVVGLKQVGLPFGGALGALIMPPVALAFGWR
                     AAVGCSAAAVGALAVLTWALYRDLPEPETKGPAPAPPRFWAVMANRDLWLVGVSTLIF
                     AGVQTVFLAFLVLYLRDVVHIALVVAAKYLVAAQVSGAIGRIGFGLLSDRLFGGRRRI
                     VLAIAGLGIDRLRPRPVGHRAGDGALAARAARGGHRVLRRRLERRAAHPHGRAGRAPR
                     DRDRGGARPRHLLAGCRGVPAGVRAGGGAPGRLRRGMARAGRRHGARARTARPGARAG
                     PGADLMTGRPQHA"
     gene            complement(2287..3018)
                     /gene="kdsB"
                     /locus_tag="VNU03_03180"
     CDS             complement(2287..3018)
                     /gene="kdsB"
                     /locus_tag="VNU03_03180"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HWP73481.1"
                     /translation="MIILGVIPARLHSSRLPRKVLREIRGVPMVVHVFRQARTSSLLS
                     DVLVATDSEEVVETCHAHHVPAVMTRADHASGTDRLWEVSRARSADVYVNIQGDEPLI
                     TAAHIECLVRPFLAEPEVQVTTLKIRATVAEVPSRTANKVVTNVHGDALYFSRLPIPF
                     DRDGRGDVVYWKHIGLYAYRRAVLDTYHALPASSLERAEQLEQLRLLEAGIPIRVLET
                     DQPTIGVDTEEDLRAVEALLSARAR"
     gene            3212..>3516
                     /locus_tag="VNU03_03185"
     CDS             3212..>3516
                     /locus_tag="VNU03_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73482.1"
                     /translation="MSNGGEELIRARPAAVATVRTAASGGWSHHEAFQGFLWIAPAFL
                     YLAFFIAYPFFMSLYLSVSSARVGSPDSHFVGLQNYTRLFADPTFWQTVRNSFVFTLG
                     "
BASE COUNT          565 a         1223 c         1219 g          509 t
ORIGIN      
        1 tgaacttgac cgaatcgagc gctcccttgc tgtcgaggac gaccttcttg ccgctcttgt
       61 cagtctcggc tcccccgaag gcccacagca tggtgtaggt gaaggtgggg gcgtcgccga
      121 aggtgtgacc gagggtctgc ccgtagggct tgcccttctt cttgagcacc gcgaagacct
      181 tgcgtgcctc gtcccacgtc ttcggatact cgttggcgcc agcttccttg agccacgact
      241 tccgatgcgc cacggcattg ccgacgatcg agtggggaac cgcgagccat ttgccgccga
      301 cctggcagga ggggggatag atctcgtaga agccgccctc ggcctttccg atgtcgcccg
      361 ccacatcgct cacgtccacc accgcgttct gatagagatg ggcccagttg tactggaagt
      421 tgaagatgtc ggcgcccgcg ccggactgga tggccgcggt gatgcgcggc tgcaggtcgt
      481 tggcgttgat ggtctcgaac gtcacctccg ctccgagggc cttggacgcc tccggcatct
      541 gccgcttcag ctccacgtcg gactcgggga tgaagtccac ccagcggacc cagtggagct
      601 tggtcccctg cgcgaaggcg ggtgcgcggc gcgccgcgag gatgccctcg atgcccccca
      661 tcgcggccgc ggcgacaccc gcggtcttga tgaacgtgcg gcggtcgagc tgcgagagcg
      721 tggtgtcctt ggtctccgga ttcttcatga gcgagtggtc ctccttgacc gggtcaaccg
      781 gcggtaccgg gctgtcgttc cgcggcggct gcgccgcgcg tgctttcccc atcggggtag
      841 gagccccggc cgcgaggctg ggacctcgag aacgtcgtca ctgggatgcg agtcgagtgg
      901 tgagataggc cctaaatggt ggccgattct taacaaagaa ccatgggcct gtcaacggga
      961 aaccggggga aacggctcgg tcagcccccg acggcgccct cggtgtcacc cccagaacaa
     1021 ccgccatcaa tggcaccgcg caggacccgg aagtgagctg ccgccatgtg ggtcacgcgt
     1081 gctgtggccg cccggtcatg aggtcggcgc caggacccgc tcgcgcaccg ggacgagcag
     1141 tgcgagcgcg agcaccatgc cgacggccag cgcgagccat cccgcgccga agccgcccag
     1201 gcgctccacc accagcccga acaccggcgg gcacaccgcg acacccagcg aggagatggc
     1261 gaggccgagc cccaccgcgg tcccggtcgc gcggggcccg gccagctcgg ccatgagggt
     1321 gtgctgcacg ccgttccagc cgacgccgaa gaacccgatg cccaccgcga gcggcgcgag
     1381 cagccagggc cccgtccccg gcgcggtggc cgacagggcg agggcgcagc cgatcgatcc
     1441 ccaggcccgc gatggccagc acgatgcggc ggcggccgcc gaagagccgg tcggagagca
     1501 gcccgaaccc gatgcgcccg atggcgccgc tgacctgggc cgccaccagg tacttggcgg
     1561 ccaccacgag ggcgatgtgc acgacgtcgc ggaggtagag gacgaggaac gcgaggaata
     1621 cggtctgcac gcccgcgaag atgagggtgc tgacccccac cagccacaga tcgcggttgg
     1681 ccatcacggc ccagaagcgt ggaggcgccg gagcgggtcc cttcgtctcc ggctccggca
     1741 ggtcccggta gagcgcccag gtcaggacgg cgagcgcccc gaccgccgcg gccgagcacc
     1801 ccaccgcggc ccgccacccg aaggcgagcg ccaccggagg catgatcagg gcgcccagcg
     1861 cgccgccgaa cggcaagccg acctgcttga gaccgaccac ggtcgcccgc tgccgcggtg
     1921 ggaaccaggc catgcctgcc ttggtggtgg tggggttcag cacgccgtag ccggcgccgg
     1981 ccaccaccag gatcgcgacg atgaacgaga aggacggcgc caccgccacc gcgccgagcc
     2041 cgaccgcgat cagggcttgc cccaggatca tcgccccgcg cacgccccag cggtccgcca
     2101 gccaccccgc gggcagcgac atcagcaccg gcccgatgta gtaggccgag aggaaggagc
     2161 cggcctgcgt caccgacagc ccgaggctct cccggatcag cggcgcgatc gacgggatgc
     2221 cgagcgggcc cacgttggcc atggtctgcg cggccatgat gagggccagg acggcccagc
     2281 ggttggtcac cgggcgcgag ccgacaacag ggcctcgacg gcccggaggt cctcctcggt
     2341 gtccactccg atggtgggct ggtcggtctc gaggacccgg atggggatcc cggcctccag
     2401 caggcgaagc tgctcgagct gctccgcgcg ctcgagcgag gacgctggca gcgcgtggta
     2461 cgtgtcgagg accgcgcgcc ggtaggcgta gaggccgatg tgcttccagt agacgacgtc
     2521 gccgcgcccg tcccggtcga aggggatcgg caggcgcgag aagtagagcg cgtcgccgtg
     2581 cacgttggtg accaccttgt tggcggtgcg cgacggcacc tccgcgacgg tggcccggat
     2641 cttgagcgtc gtcacctgca cctcgggctc ggccaggaag ggccggacca ggcactcgat
     2701 gtgcgcggcg gtgatcagcg gctcgtcgcc ctggatgttg acgtagacgt cggcgctccg
     2761 ggcgcgcgac acttcccaca gccggtcggt gcccgaggcg tggtcggccc gggtcatgac
     2821 cgcgggcacg tggtgggcgt ggcaggtctc caccacctcc tcgctgtcgg tggccaccag
     2881 cacatcggag agcagggagg aggtgcgggc ctgccggaag acgtggacca ccatggggac
     2941 gccgcgaatc tcccgcagca ccttgcgcgg cagccgtgaa gaatgcaggc gcgccgggat
     3001 cacgccgaga atgatcaccg cgttacccta tcagaaaaca acacctggcg aggttgacac
     3061 tcctcgggag ccaatggtat aacccgtgaa cctcgcatcg atgtcgctcg cttcctcacc
     3121 gatccatctc gaaggagaga caagggatcg tgcaacagag tatgagccgc agtgcgtcag
     3181 cggcccggtc cgtccggtcg gagactagtt cgtgagcaat ggcggcgagg agctgatccg
     3241 cgcccgcccg gccgcggttg ccacggtgcg caccgccgcg tcgggcgggt ggagccatca
     3301 cgaggccttc cagggcttcc tgtggatcgc gcccgccttc ctctacctcg cgttcttcat
     3361 cgcctacccg ttcttcatgt cgctctacct ctcggtctcg agcgcccgcg tgggctcgcc
     3421 cgacagccac ttcgtggggc tgcaaaacta cacgcgcctg tttgccgacc cgaccttctg
     3481 gcagacggtg cgcaattcct tcgtcttcac cctggg
//
LOCUS       DATMCV010000132         3080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_74427, whole genome shotgun sequence.
ACCESSION   DATMCV010000132 DATMCV010000000
VERSION     DATMCV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74427"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="VNU03_03190"
     CDS             complement(<1..1515)
                     /locus_tag="VNU03_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP73483.1"
                     /translation="MHRPASHTLPDLLDELAARQPEHELIVDATGRVRLGYAETRARA
                     RRLARGLVRLGVRRGDRVALLMTNRPEWLLIDFAVTLLGAVLVPISTWSRPRELEYVL
                     GHCATSVLLLAPRFGSQDYLAALAEMGGAGSARLPHLRRLVVCGDEAARAPAGALPSL
                     EALAELGRDVSDAELDAAQRAVTPDDVAYILYTSGTTSTPKGVQLAHGGLIENGWHIG
                     ERQHLTPADRMWMGISLFWSFGCANSLPAVMTHGGTIVLQEQIEPAATLALIERERCT
                     VYYGTPNIAQALIEHPDRARRDLSSLRTGAAIGTPAAMQMVMDLGAREICNVYGLTEC
                     YGNSSVTDAHDPPEVRRTTVGFPLPGMEIRVVDRESRRPLPDGEVGEILIRGRLTPGY
                     YQDPERNASAFDAEGFLLSGDLGFVGPDGRLRFRGRIKEMVKTGGINVAPVEVEEILL
                     GHHDVEQAYVVGIPDLRKEEILVAVVVAKVGHRIEPAALHALCKDALAAYKVPQA"
     gene            complement(1533..2192)
                     /locus_tag="VNU03_03195"
     CDS             complement(1533..2192)
                     /locus_tag="VNU03_03195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWP73484.1"
                     /translation="MTRWPWLAALLLLHAGCTSLTASQQAHLDDWQRFADRVTAHYGV
                     ANVTFLVGEQPGAGGAMRPGGLMTFQTQMLEPLPAGQTRDFLLAHELAHWVLGHAYRR
                     APDEAATSLWDAQQERRELDANAEAVKILMIGRHWSERRAFFHAHAYLWSYRRGVEAR
                     RVSVPAGHPVDTCVEINDLIRRYPTYRDISDSCPSWPEPRWADHAAQRDATSSEPLDP
                     R"
     gene            complement(2205..2741)
                     /locus_tag="VNU03_03200"
     CDS             complement(2205..2741)
                     /locus_tag="VNU03_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP73485.1"
                     /translation="MTCLIMHIEKKLDALGLTLMDLDRAYRANAAGARFVSHLAVNNV
                     LYLSGTTPVREGKPHLVGVVGADLSIEQGYEAARYALLSSLAAIKYALGDLDRVQQAV
                     QLIGFVNSAPGFSEQPRVINGATDLLVELYGDRGKPTRAAIGCQGLALGHSVEVVLTV
                     LFTGLGTTPPLARDHYVK"
     gene            2875..3069
                     /locus_tag="VNU03_03205"
     CDS             2875..3069
                     /locus_tag="VNU03_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73486.1"
                     /translation="MRQSKLTIAWRGTPDLDDWVAYIVTKTKSKKLILADQVSERKVK
                     ALLARVSGLSRRGVEKLAKG"
BASE COUNT          461 a         1055 c         1128 g          436 t
ORIGIN      
        1 cgcctgcggc accttgtagg cggcgagcgc gtccttgcag agggcgtgca gcgcggcagg
       61 ctcgatgcgg tggcccacct tcgccaccac caccgcgacc aggatctcct ccttgcgcag
      121 gtcggggatg ccgactacgt aggcctgctc gacatcatgg tggcccagga gaatctcctc
      181 gacctccacc ggcgccacgt tgatcccccc ggtcttgacc atctccttga tgcggccgcg
      241 gaagcgcaga cgcccgtcgg gccccacgaa cccgagatcg ccggacagca ggaagccctc
      301 cgcgtcgaag gccgacgcgt tgcgctccgg gtcctggtag tagcccggcg tcaagcggcc
      361 gcgaatcagg atctcgccga cctcgccgtc cggcagcggg cgccggctct cgcggtccac
      421 cacgcggatc tccatcccgg gcaggggaaa gcccacggtg gtccgccgca cctcgggcgg
      481 gtcgtgcgcg tcggtgaccg agctgttgcc gtagcactcg gtcagcccgt agacgttgca
      541 gatctcccgc gcgccgaggt ccatcaccat ctgcatcgcg gcgggggtgc cgatcgcggc
      601 gccggtgcgg agcgacgaca ggtcgcgccg cgcgcggtcc gggtgctcga tcagggcctg
      661 ggcgatgttg ggcgtcccgt agtagaccgt gcaacgctcc cgctcgatca gggccagcgt
      721 ggcggccggc tcgatctgct cctggagcac gatggtgccc ccgtgggtca tcaccgcggg
      781 cagcgagttg gcgcagccga agctccagaa gagcgagatg cccatccaca tgcggtcggc
      841 cggggtcaga tgctgacgct cgccgatatg ccacccgttc tcgatcagcc ccccgtgggc
      901 gagctgcacg cccttgggag tcgacgtggt gccggaggtg tagaggatgt acgccacgtc
      961 gtccggggtg accgcgcgct gggcggcgtc gagctcggcg tcgctcacgt cccggcccag
     1021 ctcggcgagg gcctcgagcg atgggagcgc cccggccggc gcgcgggccg cctcgtcgcc
     1081 acacaccacc aggcgccgca gatgcggcag ccgcgcggag cccgcgccgc ccatctcggc
     1141 gagcgcggcc aggtagtcct ggctcccgaa gcgcggcgcg agcagcaaga ccgacgtcgc
     1201 gcagtggccg agcacgtact cgagctcgcg gggccgcgac caggtgctga tcggcaccag
     1261 gaccgcgccg agcagcgtca ccgcgaagtc gatcaggagc cactccggcc ggttggtcat
     1321 gagtagcgcg acccgatcgc cgcgacgcac cccgaggcgc acgagcccgc gggcgagccg
     1381 ccgcgcccgc gcccgcgtct cggcgtagcc gagccgcacg cggccggtgg cgtcgacgat
     1441 cagctcgtgc tccggctggc gcgcggccag ctcgtcgagc aggtcgggaa gggtgtggct
     1501 cgcgggacga tgcacgcgga tcgggcggcg gctcagcggg ggtcgagcgg ctcgctcgac
     1561 gtcgcgtcgc gctgcgccgc gtggtcggcc caccggggct cgggccacga gggacaggag
     1621 tcgctgatgt cgcggtaggt gggatagcgc cggatcaggt cattgatctc gacgcacgtg
     1681 tcgaccgggt gccccgccgg cacgctcacg cggcgcgcct ccaccccgcg ccgatagctc
     1741 cagagataag cgtgcgcgtg gaagaaggcc cggcgctcgg accagtgacg gccgatcatg
     1801 aggatcttca ccgcctccgc gttggcgtcc agctcgcggc gctcctgctg ggcgtcccac
     1861 agcgaggtgg ccgcctcgtc gggcgcccgg cgataggcgt ggcccagcac ccagtgggcc
     1921 agctcgtgcg cgagcaggaa atcgcgggtc tggccggccg gcagcggctc gagcatctgg
     1981 gtctggaagg tcatgaggcc gcccggccgc atcgcgccgc ccgcgccggg ctgctcgccg
     2041 accagaaacg tgacgttggc gaccccgtag tgggcggtga cccggtccgc gaagcgctgc
     2101 cagtcgtcga ggtgggcctg ctgtgaggcg gtgaggctcg tgcagccggc gtgcaggagg
     2161 agcagggcgg ccagccaggg ccagcgcgtc atggtgaccg gagcctactt cacgtagtga
     2221 tcgcgcgcca ggggcggcgt ggtccctagg ccggtgaaga gcacggtgag cacgacctcg
     2281 accgagtggc cgagggccag gccctggcag ccgatggcgg cgcgggtggg cttgccgcgg
     2341 tcgccgtaca gctcgacgag cagatcggtg gcgccgttga tcacgcgcgg ctgctcgctg
     2401 aacccgggcg ccgagttcac gaatccgatg agctgcaccg cctgctgcac gcggtcgagg
     2461 tcgccgagcg cgtacttgat cgcggccagg ctcgagagca gagcgtagcg cgcggcctcg
     2521 tagccctgct cgatcgagag atcggcgccc accacgccga cgaggtgcgg cttgccttcg
     2581 cgcaccggcg tcgtgccgga gaggtagagc acgttgttga ccgcgaggtg agagacgaag
     2641 cgggcgcccg ccgcgttcgc ccgatacgcc cgatccagat ccatcagcgt gagcccgagc
     2701 gcgtcgagct tcttctcgat gtgcatgatg aggcaggtca taacatggtt atccacactt
     2761 attcacaaaa ctttcttcgt catcggcttg acacatcccc cgcacgttca ctaggcttca
     2821 cgagtcatgc aggggagtcg aaagccgttg gcgaaggtcg aaggacgccg gtcgttgcgg
     2881 cagagcaagc tcaccatcgc gtggcgcggc acgccggacc tcgatgactg ggtcgcgtac
     2941 atcgtgacga agacgaagtc gaagaagctg atcctcgcgg atcaggtgtc ggagcggaag
     3001 gtgaaggccc tgctcgcgcg cgtgtcgggg ctctcccggc gcggcgtgga gaagctggcg
     3061 aagggttagg ccgagcaacc
//
LOCUS       DATMCV010000133         4577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_273255, whole genome shotgun sequence.
ACCESSION   DATMCV010000133 DATMCV010000000
VERSION     DATMCV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273255"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /gene="lptA"
                     /locus_tag="VNU03_03210"
     CDS             <1..420
                     /gene="lptA"
                     /locus_tag="VNU03_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HWP73487.1"
                     /translation="DQLENIQKEGLVVFTGNVVATQNGSTQWAERMEVYLDDKGDRIV
                     RTVSTGNVRIITRDCRSGTAKRAEYFDAEQRVVLIGNARVWRDDNVVTGERITIYLAE
                     DRSLVEGGQQERVKAVFYPKSQDEAAARPKPVAGQCS"
     gene            428..1150
                     /gene="lptB"
                     /locus_tag="VNU03_03215"
     CDS             428..1150
                     /gene="lptB"
                     /locus_tag="VNU03_03215"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HWP73488.1"
                     /translation="MEGLIAQGLRKRFRSRVVVNRVSLDIHPGEVVGLLGPNGAGKTT
                     SFYMIVGLLRPDGGRIFLEGEDVTELPMYKRCRLGMGYLPQESSVFRKLTVEENLLAI
                     LETLDLSHHERMTRLGELLAELDLTRLARHKAFTLSGGERRRLEITRALVTNPRYLLL
                     DEPFTGIDPIAIGDIQEIVARLKERGIGVLITDHNVRETLAITDRAYILYDGRILASG
                     TATELANNSKAREIYLGEKFSL"
     gene            1147..2622
                     /gene="rpoN"
                     /locus_tag="VNU03_03220"
     CDS             1147..2622
                     /gene="rpoN"
                     /locus_tag="VNU03_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWP73489.1"
                     /translation="MKLVQRMSQRVVMTPLLQQAIQLLQLSTLELEQVVRKELEENPL
                     LEEIPVENPETAEATAASTSTTDASAPAETAPAPEAPEQPSKETSSVDGERADDLPFD
                     LSSAIFDDHGDERTPVSMEERDDLPFENLGRSDTSLTEHLMEQLRLSGADKDVLRIGE
                     AIIGNLDEDGYLRADVGEIAAGMGCSADAVEAALKRIHAFDPPGVAARSVQECLLLQL
                     TADAEPDPVSVEIVEKHFEDLERRRYAEIARVLKLPLERVMESIEEIQSLEPKPGRRF
                     STNDSRYVVPDVTIQKVGEDYVVILNEEGIPRLRVNSLYRSLLRRSGDEAKQYVEQKL
                     RSAVWLIKSVEQRQRTLRKVAQSLVNFQRDFLDKGLPYLRPLALRDVGDDISMHESTI
                     SRVTTNKYVQTPQGLFELKFFFHSGIASENGSMVSSVSVKKTIRDMVSGEDNGKPLSD
                     QEIAQALQGQGLTIARRTVAKYREELGILPSHQRRLQSKKR"
     gene            2627..2935
                     /locus_tag="VNU03_03225"
     CDS             2627..2935
                     /locus_tag="VNU03_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HWP73490.1"
                     /translation="MSAVQVIISARGMTVSRSYKDELTRKLAKLEPMLPAPVATRAIL
                     TREKHRRTAALTLVTRSRAFRSRETAPDLAVAVDRAVLALRRQVRETTKRRPRRPTRP
                     "
     gene            2932..3813
                     /gene="rapZ"
                     /locus_tag="VNU03_03230"
     CDS             2932..3813
                     /gene="rapZ"
                     /locus_tag="VNU03_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWP73491.1"
                     /translation="MTDPGPEPIRFVIITGLSGAGKSYAIKCLEDLGYFCVDNLPTTL
                     IPTFAELCANSSRGIQAIALGVDVREGEYLVNMVETIQELRNRGHRVDVLFLEASDET
                     LVRRYHETRRRHPLAGEGNVIDGIRAERAALAHLREIANRVIDTTSLTVHQLKDQMVH
                     SYGPQAAKGGLTVSLVSFGFKHGVPYDADLVFDVRFLPNPHFVERLRALDGRDPAVEE
                     FVMSFSESRELLSRLEGLLKFLLPLYEREGKAYLTVAIGCTGGRHRSVMLVEALRSFL
                     TGLGLSPIVRHRDLDRE"
     gene            3806..>4577
                     /locus_tag="VNU03_03235"
     CDS             3806..>4577
                     /locus_tag="VNU03_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73492.1"
                     /translation="MSDDRLHFLLGIHNHQPVGNFDSVVDDAVVRAYHPFLETLDRAG
                     GGLPLTVHCSGGLLEALKARARPTFDLLGRLAADGRVELLGGGFYEPILALLPDWDKV
                     GQIQALAHFLKSHWGVTPRGMWLAERIWEPQLPRVLREAGVEFVLVDDSHFALAGLDP
                     ETLGGYYLTEEQGASVAVFPINQRLRYLVPFSDPGESLRYLEGRRGAGAVTMVDDGEK
                     FGAWPGTDRLVYGERWLERFLDALRAAPWLHVSTFSRYL"
BASE COUNT          730 a         1641 c         1545 g          661 t
ORIGIN      
        1 gaccagctcg agaacatcca gaaggaaggg ctggtggtgt tcaccggcaa cgtggtggcc
       61 acccagaacg gctccaccca gtgggccgag cgcatggagg tctacctcga cgacaagggc
      121 gaccgcatcg tgcgcaccgt ctccaccggc aacgtgcgca tcatcacgcg ggactgccgt
      181 agcggcaccg cgaagcgcgc cgagtacttc gacgccgagc agcgcgtggt gctgatcggc
      241 aacgcgcggg tctggcgcga cgacaacgtg gtgaccggcg agcggatcac gatctacctg
      301 gccgaggacc gcagcctcgt cgagggcggt cagcaggagc gggtgaaggc ggtcttctac
      361 cccaagtccc aggacgaggc cgccgcgcgt cccaagccgg tggccggaca gtgctcgtga
      421 ggcgcacgtg gaaggtctga tcgcccaggg cctgcgcaag cggtttcgca gccgcgtggt
      481 ggtcaaccgc gtgagcctcg acatccaccc cggcgaggtg gtgggcctcc tcgggcccaa
      541 cggggcgggc aagaccacgt cgttctacat gatcgtgggg ctgctgcggc cggacggcgg
      601 gcggattttc ctcgagggcg aggacgtgac cgagctgccg atgtacaagc gctgccggct
      661 cggcatgggc tacctgccgc aggagtcctc ggtgttccgg aagctgaccg tcgaggagaa
      721 cctgctcgcg atcctcgaga ccctcgacct ctcgcaccac gagcgcatga cccgcctcgg
      781 ggagctgctc gcggagctcg atctcacccg gctcgcccgc cacaaggcct tcaccctctc
      841 cggcggcgag cggcggcgcc tcgagatcac ccgggccctc gtgacgaacc cgcgctacct
      901 gctgctcgac gagccgttca ccggcatcga tcccatcgcg atcggcgaca tccaggagat
      961 cgtggcccgg ctgaaggagc gcggcatcgg ggtgctgatc accgaccaca acgtgcggga
     1021 aacgctcgcg atcaccgacc gtgcctacat cctctacgac ggccgcatcc tcgcctccgg
     1081 caccgcgacc gagctggcca acaactccaa ggcccgagag atctacctgg gcgagaagtt
     1141 ctccctatga aactcgtaca gcggatgagc cagcgggtcg tcatgacccc gttgctgcag
     1201 caggcgatcc agctcctcca gctctccacc ctcgagctgg agcaggtcgt gcgcaaggag
     1261 ctggaggaga atcccctcct cgaagagatc ccggtggaga acccggagac cgcggaggcc
     1321 accgcggcct ccacgagcac caccgatgcc tccgcccccg cggagacggc gcccgccccg
     1381 gaggcgcccg agcagccgtc caaggagacg tcgtcggtgg acggggagcg ggccgacgac
     1441 ctgcccttcg acctctcctc cgcgatcttc gacgaccacg gcgacgagcg caccccggtg
     1501 tcgatggagg agcgcgacga tctgccgttc gagaacctgg gccgcagcga cacctccctg
     1561 accgagcacc tcatggagca gctgcgcctg agcggcgccg acaaggacgt cctccgcatc
     1621 ggggaggcca tcatcggcaa cctcgacgag gacggctacc tccgcgccga cgtgggcgag
     1681 atcgcggccg ggatgggctg cagcgcggac gcggtggagg cggcgctgaa gcggatccac
     1741 gccttcgatc cgcccggggt cgcggcgcgg agcgtgcagg agtgcctgct gctgcagctc
     1801 accgcggacg cggagcccga tccggtgtcg gtcgagatcg tcgagaagca cttcgaggac
     1861 ctcgagcgcc gccgctacgc cgagatcgcc cgcgtcctga agctgccgct cgagcgggtg
     1921 atggagtcga tcgaggagat ccagagcctc gagccgaagc ccggccggcg cttctccacc
     1981 aacgactccc gctacgtggt gcccgacgtc accatccaga aggtgggcga ggactacgtg
     2041 gtgatcctga acgaggaggg catcccccgt ctccgggtca actcgctcta ccgctccctg
     2101 ctgcgccggt cgggggacga ggccaagcag tatgtggagc agaagctgcg cagcgcggtg
     2161 tggctgatca agagcgtcga gcagcgacag cgcacgctcc ggaaggtcgc ccagagcctg
     2221 gtcaacttcc agcgcgactt cctcgacaag ggcctgccct atctgcggcc gctcgccctg
     2281 cgcgacgtgg gtgacgacat cagcatgcac gagtccacca tcagccgggt caccaccaac
     2341 aagtacgtcc agactccgca ggggctcttc gagctgaagt tcttcttcca cagcggcatc
     2401 gcctccgaga acggctcgat ggtctcgtcg gtatcggtga agaagacgat ccgcgacatg
     2461 gtgagcggcg aggacaacgg caagccgctc tcggaccagg agatcgccca ggcgctccag
     2521 ggccagggcc tgaccatcgc ccgacgcacg gtggccaagt accgggagga gctgggcatc
     2581 ctgccgtcgc accaacggcg gctgcaatcc aagaagcgct gagggcgtgt ctgccgtgca
     2641 ggtgatcatc agcgcccgcg ggatgaccgt ctcccgctcc tacaaggacg agctgactcg
     2701 caagctcgcc aagctcgagc cgatgctccc cgcgccggtg gcgacgcgcg cgatcctcac
     2761 ccgggagaag catcggcgca ccgcggccct caccctggtg acccgctccc gcgctttccg
     2821 gagccgcgag accgcgcccg acctggccgt cgcggtggac cgggcggtcc tggccctccg
     2881 ccggcaggtg cgcgagacca ccaagcgccg tccccgccgc ccgacccgcc cgtgaccgat
     2941 cccggccccg agcccatccg gttcgtcatc atcaccggcc tgtccggggc ggggaagagc
     3001 tacgcgatca agtgcctcga ggacctcggc tacttctgcg tggacaacct gccgaccacc
     3061 ctcatcccca ccttcgcgga gctctgcgcc aactcgagcc gcgggatcca ggccatcgcg
     3121 ctcggggtcg acgtgcggga aggggaatac ctggtcaaca tggtggagac gatccaggag
     3181 ctgcggaacc ggggccaccg ggtcgacgtg ctcttcctcg aggcctccga cgagacgctg
     3241 gtgcgccgct accacgagac gcggcgccgg catcccctcg ccggcgaggg caacgtgatc
     3301 gacggcatcc gggccgagcg cgccgcgctg gcccacctcc gggagatcgc caaccgggtc
     3361 atcgacacca cctcgctgac cgtgcaccag ctcaaggacc agatggtgca ctcctacggc
     3421 ccgcaggcgg cgaagggcgg tctcaccgtg tccctggtct ccttcggctt caagcatggc
     3481 gtgccctacg acgccgacct cgtcttcgac gtgcgcttcc tgcccaaccc ccacttcgtg
     3541 gagcggctcc gcgccctcga cggccgcgat ccggcggtcg aggagttcgt catgtcgttc
     3601 tccgagagcc gggagctgct ctcgcgcctg gagggcctgc tgaagttcct cctgccgctc
     3661 tacgagcgcg agggcaaggc ctacctcacg gtggccatcg gctgcaccgg gggccggcac
     3721 cgctcggtga tgctggtgga ggcgctccgc tccttcctga ccggtctcgg cctgagtccg
     3781 atcgtcaggc accgggacct ggaccgtgag tgacgaccgg ctccacttcc tcctcggcat
     3841 ccacaaccac cagccggtcg gcaacttcga ctccgtcgtc gacgacgcgg tcgtccgggc
     3901 ctaccacccc ttcctcgaga cgctcgatcg cgccggaggc ggcctgccgc tgaccgtgca
     3961 ctgctcgggg ggcctgctcg aggccctcaa ggcccgcgcg cgtccgacct tcgacctgct
     4021 ggggcgcctg gccgccgacg ggcgggtgga gctgctcggg ggtggcttct acgagccgat
     4081 cctggcgctc ctgccggact gggacaaggt gggccagatc caggcgctcg cccacttcct
     4141 caagtcgcac tggggcgtga ccccgcgcgg gatgtggctg gccgagcgga tctgggagcc
     4201 gcagctgccg cgggtgctcc gggaggccgg cgtggagttc gtgctcgtcg acgactctca
     4261 cttcgctctc gccgggctcg acccggagac gctcgggggc tactacctga ccgaggagca
     4321 gggcgcgtcg gtggcggtgt tcccgatcaa ccagcgcctg cgctacctgg tgccgttctc
     4381 ggacccgggc gagagtctcc gctacctcga gggccggcgc ggggcggggg cggtcaccat
     4441 ggtcgacgac ggcgagaagt tcggggcgtg gccgggcacc gaccggctcg tctacggcga
     4501 gcgctggctg gagcgcttcc tggacgcgct gcgcgccgcg ccgtggctcc acgtctccac
     4561 gttctcgcgc tatctcg
//
LOCUS       DATMCV010000134         3898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_141411, whole genome shotgun sequence.
ACCESSION   DATMCV010000134 DATMCV010000000
VERSION     DATMCV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141411"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VNU03_03240"
     CDS             complement(<1..420)
                     /locus_tag="VNU03_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWP73493.1"
                     /translation="MDASVELVKHLLASVVHIHTEVPSSHPSTRILGDERMGTGTVVD
                     PAGLILTVNYVVMGGETIQVTFGKGRAMRAELVAQDFETGLALLRVKRQGLPAVPIAD
                     SQSIDRGTPVFALGSTGPRERRVAGGLVTYLGEYAGYW"
     gene            complement(511..1365)
                     /locus_tag="VNU03_03245"
     CDS             complement(511..1365)
                     /locus_tag="VNU03_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWP73494.1"
                     /translation="MRRTIATLIVAVVLAAGCSSLRGGQAISFDSATPGAPLAIDATL
                     VLPSGPGPFPAVVQLHGCAGVEAQSFRWARWLADHGYAALVVDSLRARHVKGDCRTGP
                     DEPPVTARFDDAFGALRYLQSRPDVRADRVAAIGWSQGGVYAMAVINGPSLERARRRG
                     VELPAVGFAASVGVYPGGCFSLVKEQVVRPLLVLIGEADDWTPAATCREMVAAMHSRG
                     ADATIVTYPGAYHYFDVEGQPLEVLAHVENDNRAGGYGATVSYQAAAAADAHRRIEEF
                     LARHLRRP"
     gene            1450..1944
                     /locus_tag="VNU03_03250"
     CDS             1450..1944
                     /locus_tag="VNU03_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP73495.1"
                     /translation="MTEDPTREIRAEAQRHFQQAYEAHMQGRLEEASAHYQRSIALHP
                     TAEAHTFLGWVYSHQGRPDDAIAQCKIAISVDPEFGNPYNDIGAYLIEMGREEEAVEW
                     LERAKQAARYEPRHYPYFNRARVHVKRHEIREAIGELERALSLEPRYVTARRELHRLR
                     GLLN"
     gene            1941..>3898
                     /locus_tag="VNU03_03255"
     CDS             1941..>3898
                     /locus_tag="VNU03_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HWP73496.1"
                     /translation="MTSIPLPFYDNPLLFGHAGTRGLLAFRPSESHVTVYARGADGVI
                     VTEEPFRPFLLLADPDLLKGFKGELEVTPLDGDGTYRWLAEFSAWSQALRARDHCLRA
                     SGKGSGAPDAPYRFLADPTHQFLLTSGRTSFLGMAFGDLRRMALDIEVTTGAGFEFPN
                     AARESDRIIAIAIADSTGFTAVLSGAHLSEAELLVECSRLIRERDPDVLEGHNVFRFD
                     LEYLEARARRHRVPLTWGRDGSILRGFPSRMQVAERTIGYRRYGVTGRHIVDTWILAQ
                     LYDVGARDLESYGLKDVARHFGIAAPDRTYLPPEEIPRIFLDDPARLMAYARDDVIET
                     LGLSAILSPPYFVQAQSLPFDYQTTVLRGNATKIDALLMREYLHRRQAVPAPGRGRGV
                     AGGYTAMFQRGVARDVLHVDVTSLYPSLMLTQALFPASDRLGVSASLLRDLREFRVSA
                     KRLAREAAAPEDRIFLNALQQTFKIFINSFYGYLAFSQGHWNDFDVANQVTGEGRRLV
                     QVLLDRLAALGATVIEVDTDGIYFVPPAGDAEDPAALEERLVADLSAALPEGIQLELD
                     GRYVAMLSYKMKNYVVLDGRGKLLIKGSGLRSRGIELFQRLWMEQMFRLLLTGRRAEI
                     PDLVRRWEADFIAHRVPLKQFMKTETLQ"
BASE COUNT          564 a         1446 c         1346 g          542 t
ORIGIN      
        1 ccagtagccc gcgtactcgc ccaggtaggt cacgagcccg cccgccaccc ggcgctcgcg
       61 ggggccggtc gagccgaggg cgaaaaccgg cgtgccccga tcgatcgact gcgagtcggc
      121 gatcgggacc gcggggaggc cctgacgctt cacgcgaagc agggcgagcc cggtctcgaa
      181 gtcctgggcc accagctcgg cccgcatggc gcggcccttc ccgaaggtga cctggatggt
      241 ctcgccgccc atcaccacgt agttgacggt gaggatgagt ccggcggggt ccaccaccgt
      301 cccggtcccc atgcgctcgt cgcccaggat gcgcgtggag ggatgggacg acggaacctc
      361 ggtgtggatg tgtaccaccg aggcgagcag atgcttgacg agctcgaccg acgcgtccat
      421 ggagatgatg cagtcgggct cacgctagca cgcgccgggc gcaccgtgca acgcgcgcct
      481 tgcgcggacg gccctcggcg cgcgcgccgg tcacggccgg cgcaggtggc gggccaggaa
      541 ttcttcgatc cggcggtggg cgtcggcggc ggccgccgcc tggtaggaga cggtggcccc
      601 gtagccgccc gcccggttgt cgttctcgac gtgggccagc acctcgagcg gctggccctc
      661 gacgtcgaag tagtgatagg cgccgggata cgtgacgatg gtggcgtcgg cgccgcgcga
      721 gtgcatcgcc gcgaccatct cccggcacgt cgcggccgga gtccagtcgt cggcctcgcc
      781 gatgagcacc agcagtggcc gcaccacctg ctccttcacc agcgagaagc agccgccggg
      841 gtacaccccc acgctcgccg cgaagccgac cgcgggcagc tccacgccgc gccggcgcgc
      901 gcgctcgagg ctcggcccgt tgatgaccgc catcgcatag acgccgccct gcgaccagcc
      961 gatcgcggcc acgcggtccg cccggacgtc gggccgcgac tgcagatagc gcagcgcccc
     1021 gaacgcgtcg tcgaagcgcg cggtgaccgg gggctcgtcg gggccggtcc ggcaatcgcc
     1081 cttcacgtgc cgcgcccgga ggctgtcgac caccagggcc gcgtagccgt ggtcggccag
     1141 ccatcgcgcc cagcggaagg actgggcctc gacgccggcg cagccgtgca gctgcaccac
     1201 cgccgggaac gggccggggc cggacggcag caccagggtc gcgtcgatcg cgagcggagc
     1261 cccgggagtc gcgctgtcga aggagatggc ctgccccccg cgtaggctcg agcagcccgc
     1321 cgcgagcacg accgccacga tcagggtcgc gatggtgcga cgcacccgcc gagcataccg
     1381 caagcggtgc aggcgcggca ccattcggag catccgctgc ggtagaatgc cggccaggag
     1441 gcggccgaga tgacggagga tccgacccgc gagatccgcg cggaggcgca gcgccacttc
     1501 cagcaggcgt acgaggcgca catgcagggc cgcctcgagg aagcgagcgc gcactaccag
     1561 cggtcgatcg cgctccaccc caccgcggag gcccacacct tcctgggctg ggtgtacagc
     1621 caccagggcc ggcccgacga cgcgatcgcc cagtgcaaga tcgcgatctc ggtggatccc
     1681 gagttcggga atccctacaa cgacatcggc gcctacctca tcgagatggg ccgggaggag
     1741 gaggcggtgg agtggctcga gcgagccaag caggcggcgc gctacgagcc gcggcactat
     1801 ccgtacttca accgggctcg cgtgcacgtc aagcgccacg agatccgcga ggcgatcggc
     1861 gagctcgagc gggcgctgtc gctcgagccg cgctacgtga cggcccggcg ggagctgcac
     1921 cggctgcgcg gcctgctcaa ttgacctcga tcccgctccc gttctacgac aaccccctgc
     1981 tcttcgggca cgccggcacc cgggggctgc tcgcgttccg cccgtcggag agccacgtca
     2041 cggtctacgc ccgcggcgcg gacggggtga tcgtcaccga ggagcccttc cggcccttcc
     2101 tgctcctggc cgatcccgac ctcctcaagg gctttaaggg cgagctcgag gtgaccccgc
     2161 tcgacgggga cggcacctac cgctggctcg ccgagttctc cgcctggtcg caggccctgc
     2221 gcgcgcggga ccattgcctg cgcgcctccg gcaagggctc gggggccccg gacgcgccct
     2281 accgcttcct cgcggatccg acccaccagt tcctgctgac ctcgggccgc acctcgttcc
     2341 tcggcatggc cttcggggac ctgcggcgga tggcgctcga catcgaggtc accaccgggg
     2401 ccgggttcga gtttccgaac gcggcccgcg agtccgatcg gatcatcgcg atcgcgatcg
     2461 ccgactccac cggcttcacc gccgtgctct ccggagccca tctctccgag gcggagctgc
     2521 tcgtggagtg ctcgcgcctg atccgcgagc gcgatccgga cgtgctcgaa ggacacaacg
     2581 tcttccggtt cgacctcgag tacctcgagg cgcgcgcgcg ccggcaccgg gtgccgctca
     2641 cgtgggggcg cgacggctcg atcctgcgcg gcttcccctc ccgcatgcag gtggcggagc
     2701 gcaccatcgg ctaccggcgc tacggggtca ccggccggca catcgtggac acctggatcc
     2761 tggcccagct ctacgacgtg ggcgcccgag acctcgagtc ctacgggctc aaggacgtgg
     2821 cccgccactt cgggatcgcg gcgccggacc ggacctatct gccccccgag gagatccccc
     2881 gcatcttcct cgacgacccg gcgcgcctca tggcctacgc gcgcgacgac gtcatcgaga
     2941 ccctcggcct ctccgcgatc ctgtcaccgc cctacttcgt gcaggcgcag agcctgccgt
     3001 tcgactacca gaccacggtg ctgcggggca acgcgacgaa gatcgacgcg ctgctgatgc
     3061 gcgagtacct ccaccggcgg caggcggtgc ccgcccccgg gcgtggtcgc ggcgtggcgg
     3121 gcggctacac cgccatgttc cagcgcgggg tcgcgcgcga cgtgctccac gtggacgtga
     3181 cctcgctcta tccgtccctg atgctgaccc aggccctctt cccggcctcc gaccgcctgg
     3241 gagtctccgc ctcgctgctg cgcgacctgc gcgagttccg ggtctcggcc aagcggctgg
     3301 cccgggaggc cgccgctccc gaggaccgca tcttcctcaa cgcgctccag cagaccttca
     3361 agatcttcat caactccttc tacggctacc tcgccttctc gcagggccac tggaacgatt
     3421 tcgacgtggc caaccaggtg accggggagg gccggcggct cgtgcaggtc ctcctcgatc
     3481 ggctcgcggc cctcggcgcc accgtgatcg aggtggacac cgacgggatc tacttcgtgc
     3541 cgcccgcggg cgacgcggag gaccccgccg ccctcgagga gcgcctcgtg gccgatctct
     3601 ccgcggccct ccccgagggg atccagctcg agctggacgg gcgctacgtg gcgatgctca
     3661 gctacaagat gaagaactac gtggttctcg acgggcgcgg caagcttctg atcaagggct
     3721 cggggctgcg ctcgcgcggg atcgagctgt tccagcggct ctggatggag cagatgttcc
     3781 gcctcctcct gaccgggcgg cgcgcggaga tcccggacct cgtgcggcgc tgggaggcgg
     3841 acttcatcgc ccaccgggtc cccctcaagc agttcatgaa gaccgagacg ctgcagga
//
LOCUS       DATMCV010000135         2345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_273402, whole genome shotgun sequence.
ACCESSION   DATMCV010000135 DATMCV010000000
VERSION     DATMCV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273402"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VNU03_03260"
     CDS             complement(<1..948)
                     /locus_tag="VNU03_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP73497.1"
                     /translation="MTLDPARTRTLVQQAWDGDIVPALTEYIRIPAKSPMYDAKWAEH
                     GHIDRAVTLMTDWARRRKIEGLTIDVIRLEGRTPVILMEVPGKGGETVLLYGHCDKQP
                     EMVGWAADGGPWTPVRRGDRLFGRGAGDDGYASFAALTAIETLQAQGIPHARCVVLIE
                     ASEESGSPDLPAYMEQLAERLRPDLVICLDSGCSDYDHLWATTSLRGVLSGTLTVEVL
                     TEGVHSGGASGIVPSSFRILRQVLDRLEDHRTGKILVSECHVEIPPGRLAEVRAVAGV
                     LGGKVGKAFPLMPGMRPMTEDTVEALLNNTWRPMLSVTGV"
     gene            1029..1226
                     /locus_tag="VNU03_03265"
     CDS             1029..1226
                     /locus_tag="VNU03_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73498.1"
                     /translation="MTELTPAEVRTLAAAAGLPLHGADLAEVTHRLNAFVEVLGGLAR
                     LDLTDAEPAPAPPPDLPGGLR"
     gene            1223..>2345
                     /locus_tag="VNU03_03270"
     CDS             1223..>2345
                     /locus_tag="VNU03_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP73499.1"
                     /translation="MTADLVHRSAVELAGLIRARTVSPVELVEAYLARIDRWNEALGA
                     YVTVCRERALDEARAAEQALGHGAPLGPLHGVPFAVKDQFDSAGVRTTSGSRLHRDRV
                     PTDDASVVARLRSAGGILLGKLNLTEFALGGTLTHPFGQPRNPWDRTRETGGSSSGSG
                     IAAAAALAAATLGEDTGGSVRTPAAWCGAVGLRPTWGLVPRSGCVPVAWSMDAPGPLT
                     RSVEDAALLLSIIAGPDGRDRWMRPAPGDPRADLERGAGGLSIGVVTTLVDSPETDGE
                     VREAVRRSADTLRGLGARVEDVDLPLVPMAGAVFMALADGEAAAWHHRTLATRGDEYD
                     AGTRRRLVTAALLPATLAQQAARARAAIRTQLWDALGRFD"
BASE COUNT          284 a          872 c          874 g          315 t
ORIGIN      
        1 gacgccggtc accgacagca tgggccgcca tgtgttgttc agcagggcct cgacggtatc
       61 ctcggtcatc gggcgcatgc cgggcatgag ggggaaggcc ttcccgacct tgccgccgag
      121 cacccccgcc accgcgcgga cctcggcgag ccggccgggc gggatctcca cgtggcactc
      181 cgagaccagg atcttgccgg tgcgatggtc ctcgagccgg tcgagcacct ggcgcaggat
      241 gcggaagctc gaaggcacga tgccgctcgc cccgccggag tggacgccct cggtgaggac
      301 ctccacggtc agggtgccgc tcagcacgcc gcgcagcgag gtggtcgccc acaggtggtc
      361 gtagtcgctg cagcccgagt cgaggcagat gaccaggtcg ggccggaggc gctcggcgag
      421 ctgctccatg taggcgggga gatccgggct gccgctctcc tcggaggcct cgatcagcac
      481 cacgcagcgg gcgtgcggga tgccctgggc ctgcagcgtc tcgatcgcgg tgagcgccgc
      541 gaacgaggcg tagccgtcgt cgcccgcgcc gcggccgaag agccgatcgc cgcgccgcac
      601 cggcgtccac ggcccgccgt cggcggccca gcccaccatc tccggctgct tgtcgcagtg
      661 accgtagagg agcacggtct cgccgccctt gcccggcact tccatcagga tgaccggggt
      721 ccggccctcg agacggatca catcgatggt caggccctcg atcttgcgac ggcgggccca
      781 gtcggtcatg agggtgaccg cccggtcgat gtgcccgtgc tccgcccact tggcgtcgta
      841 catgggcgac ttggccggga tgcggatgta ctcggtgagg gcggggacga tgtcgccgtc
      901 ccaggcctgc tgaaccagcg tgcgcgtgcg agcggggtcg agggtcatgg gcccgatagt
      961 atgcggaatc ggccggatct gccagccagg ttgcccccgt cgcgaccacc cggtaggatc
     1021 gcctccgcat gaccgagctc actcccgccg aggtccgcac gctggccgcc gcggccggcc
     1081 tgccgctcca cggcgccgac ctcgccgagg tcacgcatcg gctgaacgcc ttcgtcgagg
     1141 tgctcggcgg cctggcccgc ctcgatctca ccgacgcgga gcccgcgccc gccccgccgc
     1201 ccgatctgcc aggcgggctc cgatgaccgc agacctcgtc catcgctctg cggtcgagct
     1261 ggccggcctg atccgcgccc gcacggtgtc cccggtcgag ctcgtggagg cctacctcgc
     1321 gcgcatcgac cggtggaacg aggcgctcgg cgcctacgtc acggtctgcc gggagcgcgc
     1381 gctcgacgag gcgcgcgcgg cggagcaggc gctcggccac ggcgcgcccc tcgggccgct
     1441 ccacggcgtg ccgttcgcgg tgaaggatca gttcgacagt gcgggcgtgc ggaccacctc
     1501 gggctcgcgc ctgcaccgcg accgcgtgcc gaccgacgac gcctccgtgg tcgcgcggct
     1561 ccgatcggcg ggcggcatcc tcctcgggaa gctgaacctc accgagttcg cgctgggcgg
     1621 cacgctcacg catccgttcg gccagccccg caacccgtgg gaccggacgc gcgagaccgg
     1681 cggctcctcc tcgggctcgg gcatcgcggc ggcggccgcg ctcgccgcgg ccaccctcgg
     1741 cgaggacacc ggcggatcgg tgcggacccc ggccgcgtgg tgcggcgcgg tcgggctgcg
     1801 tcccacctgg ggcctggtgc cccgctctgg ctgcgtgccg gtggcttggt cgatggacgc
     1861 gccgggtccg ctcacccgct cggtggagga cgcggcgctg ctgctctcga tcatcgcggg
     1921 acccgacggg cgcgatcgct ggatgcggcc cgcgcccggt gatccgcgag ccgatctcga
     1981 gcgcggcgcg ggcgggctct cgatcggcgt cgtcaccacg ctggtcgaca gcccggagac
     2041 cgatggggag gtgcgcgagg cggtgcgccg gagcgcggac accctgcgcg ggctcggagc
     2101 gcgggtggag gacgtcgacc tcccgctcgt gccgatggcg ggcgcggtgt tcatggcttt
     2161 ggccgacggc gaggccgcgg cctggcacca ccggacgctg gccacgcgtg gcgacgagta
     2221 cgacgcgggc acgcggcgcc ggctcgtcac cgcggcgctc ctgcccgcca cgctcgccca
     2281 gcaggccgcg cgggcgcgcg cagcgatccg gacgcagctc tgggacgcac tcgggcgctt
     2341 cgacg
//
LOCUS       DATMCV010000136        12011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_205912, whole genome shotgun sequence.
ACCESSION   DATMCV010000136 DATMCV010000000
VERSION     DATMCV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12011
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205912"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VNU03_03275"
     CDS             <1..400
                     /locus_tag="VNU03_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73500.1"
                     /translation="RVAMMEMGARQRMVNERALPPGGLLLINVQSTLLDADGSLWRIL
                     HPTGLGGRYWAGSQPGHRFHEVMEQARYTLDPRKRKALYVEATQLFDDEKPWLELFQE
                     VIVYGTSRRVTFRPRPDFRLIVSEMTLAPR"
     gene            complement(408..1454)
                     /locus_tag="VNU03_03280"
     CDS             complement(408..1454)
                     /locus_tag="VNU03_03280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWP73501.1"
                     /translation="MSPTPQLTAADHTARSSSRPTLPVAVIITAHGSARYLEQALASI
                     RDQTAIPAETVVVDDTFPGVDVARVIETAGVGRLRHPLGPNVGRNVAILEARQPWIAF
                     LDSEDTWAPHKLETQWRAIQACPRLDVVFANSWEVSEARRTTRPFLEQKSHYWAVERR
                     EIAPGVQECEPRSLVRQFLLGNFLSRSTLLARREVLVRVGLFDRELVHLEDRDCWLRL
                     LAAARFGVLEEPLMSGRLDVDAPDPDRRRWPYEEALDTVRLWQLMATHPTEYPPEAVA
                     FYAAERWRPLLAVGRLAEADGLVREARRHYLRAWWLGGGTWPLVRAAASYLPTPVRDG
                     LAAIRALLRRARDE"
     gene            complement(1675..2700)
                     /locus_tag="VNU03_03285"
     CDS             complement(1675..2700)
                     /locus_tag="VNU03_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWP73502.1"
                     /translation="MRLTSLMGFLADDLAVDLGTANTLVYVRGQGIVLNEPSIVAMKD
                     GAVLAAGHEAKAMLGRAPGTISVVRPLRHGVIADFDSTEKMLHYFIAKTRQRRSIVHP
                     RVVVTVPSGITQVEKRAVRQSARQAGAREAFLIEEPIAAALGAGLPVAEPGANMIVDI
                     GGGTTEVAVMSLSGIVYCKSVRIAGDEMNASVIQHIRKSYSLLVGERHAEEIKLKLGS
                     AYPIPGEHLTMEVKGRDLIVGIPKTILVTGEEIREALREPIMAIVSAVHTCLEQIPPE
                     LAADIVDKGIVVSGGGALLRGLGPLLHDQTGLPIRVSDDPLTCVVRGAGALLDDVKLL
                     SRVALPS"
     gene            2984..3757
                     /locus_tag="VNU03_03290"
     CDS             2984..3757
                     /locus_tag="VNU03_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73503.1"
                     /translation="MEVTRKPLAKIEGRKRMQVSGLTVSWHGTRSLKDWVAYVEDGDD
                     HGRLVLAHQVSERTVKGLLARIQNLSRDELHAMGRPVEAVAQQGGGPPIRRPPGTGQA
                     TPGSEIAVPATGRQLALTGDDVGESSPTERSRRRRLATAVETTAPETRRAARAGAPRD
                     LAGTVRQLRSRLETLEEEQEQLRAELAVLRGEAEVYDGPPSIFVTGWFRATLVLIVLA
                     IVVVVTVPWLMDLFEGGTREPRPPVRTDAPTGTPTPPGR"
     gene            complement(3765..4091)
                     /locus_tag="VNU03_03295"
     CDS             complement(3765..4091)
                     /locus_tag="VNU03_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73504.1"
                     /translation="MRKWIGALSLVWLLWSDLSIISKDNPLDTIVLRWLARVPRLQTD
                     QIVAGSYQTAEECKVALERHIVTLKAINDSWSAEEFTRDGDSGTIHYYPRGLEYARAD
                     LRCAEQ"
     gene            4224..4613
                     /locus_tag="VNU03_03300"
     CDS             4224..4613
                     /locus_tag="VNU03_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73505.1"
                     /translation="METILVVDDEVQVRALARDILVGAGYRVLEAEDGETALQIAEEH
                     QGAIHLLLTDIMMPGINGKELADRVAALRPDTKMIFMSGRAAEVISDAGVLIPVDAFV
                     AKPFTVERLLNKVRERLEYRSPFSRPR"
     gene            4650..5690
                     /locus_tag="VNU03_03305"
     CDS             4650..5690
                     /locus_tag="VNU03_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73506.1"
                     /translation="MASHDADPIEALFARGVADGLPAVPPTAERVAAAVAASGRGADE
                     LVAEMPPRFGRATVQKIAINAVMAGCRPEYLPVVLAAVEAMCDERFCLLGVSGTTDAV
                     APLVIVNGPIRAALDINSGAGVFGPGWRANATIGRAVRLCWVNIGGAQPGVISMSTFG
                     GPGRYTYCIGENEEASPWEPLHVEHGFAPGDSTVAVMAAEAPQIVADTRSRSAPDLLA
                     TVVRSVEVVAHHKATGLGDTALVFSPEHVTTLAADGWSKSRVREYLWTRLQKPVRELV
                     PGRDGGEGLSDEALAAYPKGARSAALVPKFQSPECLKLLVAGGAAGRFTAIVPGWPFR
                     NAPSRLVFRKLP"
     gene            complement(5687..6238)
                     /locus_tag="VNU03_03310"
     CDS             complement(5687..6238)
                     /locus_tag="VNU03_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWP73507.1"
                     /translation="MAVILEVAAMSEFHDEYSEALDGEFRKLEDALLSDQVRLLAPSE
                     PIRVSPDTTVAEAVARMVDNRRAAVVIVDAAGRLTGIFTERDLLTRVVSLGRNLAATR
                     IGDVMTPDPEALAPDDRICFAINRMHAAGYRTVPLVDARHCPIGIVTVNDVVGWLARV
                     FPEAILNLRPGDRVNRPAQMDSG"
     gene            6807..7448
                     /locus_tag="VNU03_03315"
     CDS             6807..7448
                     /locus_tag="VNU03_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73508.1"
                     /translation="MQVFVRPDRPALVERMQRLFAEDSGEVEVMLDRRRRERRSAAAA
                     YEPERRRADRRRPRVPRRVPGAAVAIGHTSRPGLDYTPLNRTQGEDRMMSVESKQVRD
                     GDEVRRWLEAGQTQLASLLELLHEHDRLRERVEASERENERLRGVTYENEQLRNRLET
                     SERQAEHLRQSMSELRGENERHQKEREDAAERLNHLVNEIAQRLRPGGGTPRS"
     gene            7658..8059
                     /locus_tag="VNU03_03320"
     CDS             7658..8059
                     /locus_tag="VNU03_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73509.1"
                     /translation="MIETEEKPRVLLVDDEEGVRMSLRALLDREYRISSVASGEAAVE
                     ELPRFRPDLVLLDVKLLPSMSGLDTLQHIKSFDERIEVVMITAFASLVTLNEALRRGA
                     FGYLIKPFPRRDLALMVRDALARRNGRVDGA"
     gene            8184..9926
                     /locus_tag="VNU03_03325"
     CDS             8184..9926
                     /locus_tag="VNU03_03325"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HWP73510.1"
                     /translation="MSLTSQVEKWVDEVAALTGPAKIAWADGSKAEYDRLVDDMMRDG
                     TLLPLNSRTYPNCYLHRSHPSDVARTEQLTFICTPTREEAGPTNNWMAPAEGKERLKG
                     LFRGSMRGRPMWVIPYLMGPAGSSMSRVGVMVTDSAYVVASMHIMTRVGAVALEHMRD
                     GDDFIRGLHSLGDLSPDRRFIMHFPEERLIWSVGSGYGGNALLGKKCHALRIASHQAR
                     TEGWMAEHMLILGLEDPEGRITYLAAAMPSASGKTNLAMVVSSLPGYRVWTVGDDIAW
                     IHVDKHGQLRATNPERGFFGVAPNTSAKTNPNGAAMVRSNTIFTNVALTPANEPWWEG
                     MGPEPPAGLLDWQGKPWTPGSGPAAHPNSRFTVPAQQCPSIAPNWEDPEGVPLSGFIF
                     GSRRARVVPLVFEAFDWDHGVFLGSAMGTETTAAITGQVGVVRRDPMAMIPFCGYNMA
                     DYFAHWLSFGPKLKNPPKIFRVNWFRRDGDGRFLWPGYGENVRILKWIVERIQGGGKA
                     VETPIGHVPAPGSLDETGLSLAPGALDAALHVDREEWRQALDELREFYEQFGERMPRA
                     IWKAHAETARRFGL"
     gene            9991..10374
                     /locus_tag="VNU03_03330"
     CDS             9991..10374
                     /locus_tag="VNU03_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73511.1"
                     /translation="MVAGLSGCASSDASFPPPQPPAAAQHFKATAPGQFPIDVYFTAW
                     GEGYVIYAPGQAPIYLISDKQGGFIIQRPGESAAFVTRRKDASGWNILRANAPATMFL
                     KNPEGEGWILQPPGELPTLIVPLPQ"
     gene            10643..11053
                     /locus_tag="VNU03_03335"
     CDS             10643..11053
                     /locus_tag="VNU03_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWP73512.1"
                     /translation="MWPWSRPSAAASRKKLTAFLDEGSDIEGKYTFTGTVLLNGKFQG
                     EIASPDTLIIGERGVVNASVRAGTLVVNGELVGNIYASERVELTRKARVHGDIESPIL
                     VLEEGGTFEGRCRMPRPDAMGSARDATVMPLKRS"
     gene            11050..11940
                     /locus_tag="VNU03_03340"
     CDS             11050..11940
                     /locus_tag="VNU03_03340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWP73513.1"
                     /translation="MSQPRGGFTLVLVHGGGSRMLHVGCPRWLLWGMFIAVTAFVALL
                     TGTWWDYFALKRAQIQAASVERRVEEQRVMLDSVEQRVADLNREVGGWQAAHLRVWST
                     FGPAPGSSVKGTGMGGAMAVLPLVFANEAPALPQALALLSSSVGESGQSLRALGRFME
                     RTRPMLLSLPLRWPVRGPLNSRFGMRQSPWSGEQEFHRGLDISANRGTAVLAPASGTV
                     YFAGSSGEYGTTVILDHGHELRSLYGHLQEIRVKPGERIDRGQVIALTGSTGRTSGPH
                     LHYEIQVHGQAVDPRQFLWD"
BASE COUNT         1814 a         4066 c         4313 g         1818 t
ORIGIN      
        1 ccgcgtggcc atgatggaga tgggagcgcg gcagcgcatg gtcaacgagc gggcgctgcc
       61 gcccgggggc ctgctcctga tcaacgtgca gtcgacgctg ctcgacgccg acggaagcct
      121 ctggcgcatc ctgcacccga ccgggctggg cggccgctac tgggcgggaa gccagcccgg
      181 ccaccgcttc cacgaggtga tggagcaggc gcgctacacc ctcgatccca ggaagcggaa
      241 ggcgctctac gtcgaggcga cccagctctt cgacgacgag aagccgtggc tcgagctatt
      301 ccaggaggtc atcgtctacg gcacctccag gcgcgtcacc ttccggcccc gcccggactt
      361 ccgcctcatc gtctccgaga tgaccctcgc cccgcgctag cccccgttca ctcgtcacgg
      421 gcgcgtcgga ggagggcgcg gatcgcggcc aacccatcgc gcaccggagt ggggaggtac
      481 gaggcggcgg ctctgacgag tggccaggtc ccgccgccga gccaccaggc gcgcaggtag
      541 tgacggcggg cctcccggac gaggccgtcc gcttccgcga gccggccgac cgcgagcagc
      601 gggcgccagc gctctgcggc gtagaaggcc accgcctcgg gtggatactc ggtcggatgg
      661 gtggccatca gctgccagag tcggaccgtg tccagcgctt cctcatacgg ccaccggcgc
      721 cggtccggat cgggggcgtc cacgtcgagg cggccagaca tcagcggctc ctcgagcacc
      781 ccgaagcggg cggcggccag gagccgtagc cagcagtcgc ggtcttccag gtggaccagc
      841 tcccggtcga acagcccgac gcgcacgagc acctcccgtc gcgccaggag cgtggagcgt
      901 gacaggaagt tacccagcaa gaactggcgc acgaggctgc ggggctcgca ctcctggacc
      961 ccgggcgcga tctcgcggcg ctccacggcc cagtagtggc ttttctgctc gaggaagggc
     1021 cgcgtggtcc ggcgcgcctc gctgacttcc cacgagttgg cgaagacgac gtcgaggcgc
     1081 gggcaggcct ggatggcccg ccactgcgtc tcgagcttgt ggggagccca cgtgtcctca
     1141 ctgtccagga aggcgatcca cggctggcgg gcctcgagaa tggccacgtt gcggcccacg
     1201 ttgggcccga ggggatggcg cagcctcccc acgccggccg tctcgatgac gcgcgccacg
     1261 tcgacaccgg ggaacgtgtc gtcgaccacg accgtctcgg ccgggatcgc ggtctgatcg
     1321 cgaatgctcg ccagcgcctg ctcgaggtag cgggccgagc cgtgggcggt gatgatcacc
     1381 gctacgggga gggtcgggcg actggatgat cgggcggtgt gatcggcggc ggtgagctgg
     1441 ggagtggggc tcatcggatg tcctccttcg gagcgctgcc tcagggcgct ctccacgaca
     1501 ctaggccatc ggacgggatg ggcgagttac gtgttggttg cgcgcggtcg cggcgagctc
     1561 gcggcgaccc ggacgctcat taggtaccgc ctggccgcgt cgcagacaag gtgggtcaac
     1621 gcgacgcgtc gcctttcgcg gcggcggggc tcggggcggt cgtccgagtg gaggctagga
     1681 gggcagggcg acgcgagaga gcagcttgac gtcgtccagc agcgcccccg ccccgcgcac
     1741 cacgcaggtg agcggatcgt cgctcacgcg gatcggcagg ccggtctggt cgtggagcag
     1801 gggaccgagg ccgcgcagca gcgccccgcc gcccgacacc acgataccct tgtccacgat
     1861 gtcggcggcc agctcggggg ggatctgctc gaggcaggtg tgtaccgcgc tcacgatggc
     1921 catgatcggc tcgcggagcg cctcgcggat ctcctcgccg gtgaccagga tggtcttggg
     1981 gatcccgacg atcagatctc gccccttgac ctccatcgtg aggtgctcac ccgggatcgg
     2041 gtaggccgag ccgagcttga gcttgatctc ctcggcgtgc cgctcgccca cgagcaggct
     2101 gtagctcttg cggatgtgct ggatgaccga ggcgttcatc tcgtcgcccg cgatccggac
     2161 cgacttgcag tagacgatgc cggacagcga catcaccgcg acctcggtgg tgccgccgcc
     2221 gatgtcgacg atcatgttgg ctcccggctc ggccaccggc agcccggcgc ccagcgccgc
     2281 cgcgatcggc tcctcgatga ggaaggcctc gcgggcgccc gcctggcgcg ccgactgacg
     2341 caccgcgcgc ttctcgacct gggtgatgcc ggagggcacg gtgaccacca cgcgtgggtg
     2401 gacgatgctc cgtcgctggc gggtcttggc gatgaagtag tggagcatct tctcggtgct
     2461 gtcgaagtcg gcgatgacgc cgtgccggag tgggcgcacg accgagatcg tgcccggggc
     2521 gcggcccagc atggccttgg cctcgtgccc ggccgcgagc accgcgccgt ccttcatggc
     2581 cacgatcgag ggctcgttca ggacgatgcc ttgcccccgg acgtacacga gggtgttggc
     2641 ggtgcctagg tcgaccgcga ggtcgtcggc gaggaagccc atgagagatg tcaggcgcat
     2701 gccccactcc tttcgcgggg gacagaacgc aacggatgtg ccagaggggc ggcatgtttt
     2761 gacgacgggg aatggttgcc cgagtctgtt cttccacaat aggcgggggt gaccggttcg
     2821 cctgcgtggt cgaaagtgtc aggtcgtgta ggttttacat gacactcgtg taaaaactac
     2881 gccggacgag cggttccaag ggccttgtca ggcggcggaa cgcgtcgtag tcttggggcc
     2941 gcgcgccggg cggcgtggga gaagggaaag gacaggacac cggatggagg tcacgcgcaa
     3001 gccactcgcg aagatcgagg gacggaagcg gatgcaggtc agcggcctga ccgtctcatg
     3061 gcacggcacc cgcagcctga aggactgggt ggcgtacgtg gaggacggtg acgatcacgg
     3121 ccgcctcgtg ctggcccacc aggtctccga gcggacggtc aagggcctcc tcgcccgcat
     3181 tcagaatctc tcccgggacg agttacacgc gatgggtcgg ccggtcgaag ccgtggccca
     3241 gcagggcggc gggcctccga tccgaaggcc tcccggcacc ggccaggcca ctccggggtc
     3301 cgagatcgcg gtgccggcga ccggtcggca gctcgccctg accggcgacg acgtcgggga
     3361 gtccagcccg accgagcgct cgcgccggcg acggctggcc accgcggtgg agacgacggc
     3421 gccggagacc cggcgcgcgg cccgcgcggg agcgccccgc gacctggccg gtaccgtgcg
     3481 gcagctccgc tcgcgtctgg aaaccctcga ggaggagcag gagcagctac gcgccgagct
     3541 ggcggtgctg cggggggagg cggaggtcta cgacgggccg cccagcatct tcgtgaccgg
     3601 gtggtttcgc gccaccctgg tgctgatcgt gctcgcgatc gtcgtggtgg tcacggtgcc
     3661 gtggctgatg gatctgttcg agggcgggac ccgcgagcca cgaccacccg tccgcaccga
     3721 cgcaccgacg ggtacaccga cgccgcccgg acgctgaggg gtcgttactg ctcggcgcag
     3781 cgaaggtcgg cgcgcgcgta ctcgagtccc ctcgggtagt agtgaatcgt tcccgagtcc
     3841 ccgtcgcgcg tgaattcctc cgccgaccag ctgtcgttga tggccttgag cgtgacgatg
     3901 tgtcgctcga gcgcgacctt gcactcctcc gcggtctggt agctgccggc gacgatttga
     3961 tcggtctgca ggcggggcac gcgggccagc cagcgcagca cgatggtgtc gagcgggttg
     4021 tccttggaga tgatggacag gtcagaccag agcagccaca ccaggctgag ggcgccgatc
     4081 catttcctca tctcccgatg actctaggac tcgcgcgcgg gcgtgtcaag gcggcgggga
     4141 gcgccgcgga acccccgggc ctggccgccg gttgaacacg gcacgtgcag gaaagcgggc
     4201 tcggggaggt agaatagccg cgcatggaga cgatcctggt ggtggacgac gaggtccagg
     4261 tgagggccct cgcccgggac atcctggtgg gcgccggcta tcgggtcctc gaggccgagg
     4321 acggggagac cgcgctccag atcgccgagg agcaccaggg ggccatccac ctgcttctca
     4381 ccgacatcat gatgcccggg atcaacggca aggagctcgc cgaccgggtt gccgccctcc
     4441 gaccggacac caagatgatc ttcatgtccg ggcgggccgc cgaggtgatc agcgacgccg
     4501 gggtgctgat tccggtcgat gcgttcgtcg cgaagccctt caccgtcgag cggctgctca
     4561 acaaggtccg cgagcgactc gagtatcgct cgcccttctc ccggccccgc tagccggcgc
     4621 ctcatccggc cccgggtccc gcctcccaca tggcgagcca tgacgccgat cccatcgagg
     4681 cgctgttcgc ccgtggggta gccgacgggc tccccgcggt gccgcccacc gcggagcggg
     4741 tggcggccgc cgtcgcggcg agcgggcggg gagccgacga gctggtggcc gagatgccgc
     4801 cgcgcttcgg ccgcgccacc gtgcagaaga tcgcgatcaa tgcggtcatg gcgggctgcc
     4861 ggccggaata cctgccggtg gtgctggccg cggtggaggc gatgtgcgac gagcgcttct
     4921 gcctacttgg tgtttccggc accaccgacg cggtggctcc cctggtgatc gtgaacggcc
     4981 ccatccgcgc cgcgctcgac atcaactcgg gggccggggt gttcggcccc ggctggcggg
     5041 ccaatgcgac catcggacgc gcggtgcgcc tctgctgggt gaacatcggc ggggcccagc
     5101 cgggggtcat cagcatgtcg accttcgggg gaccggggcg gtacacgtac tgcatcggcg
     5161 agaacgaaga ggcgagcccg tgggagccgc tccacgtcga gcacgggttc gcgcccggcg
     5221 acagcacggt ggcggtgatg gcggcggagg ccccccagat cgtcgccgac acgcggagcc
     5281 gctccgcgcc ggatctgctc gccaccgtgg tgcggagcgt cgaggtggtg gctcaccaca
     5341 aggcgacggg gctgggcgac accgcgctgg tgttctcgcc cgagcacgtg acgacgctgg
     5401 ccgccgacgg ctggtccaag agccgggtgc gggagtacct gtggacgcgc ctgcagaagc
     5461 cggtgcgtga gctggtgccg ggacgagacg gtggcgaggg gctctccgac gaggcgctcg
     5521 ccgcgtatcc gaagggcgcg cggagcgcgg cgctggtgcc gaagttccag tcgcccgagt
     5581 gcctgaagct gctggtcgcg ggcggggccg cgggccgctt caccgcgatc gtcccgggat
     5641 ggcccttccg gaacgcgccc tcccggctgg tgttccggaa gctgccctag ccgctgtcca
     5701 tctgggccgg tcggttcacc cggtcgcccg gtcggaggtt caggatcgcc tcgggaaaga
     5761 cccgcgccag ccagcccacc acgtcgttga ccgtgacgat gccgatcggg cagtgccggg
     5821 cgtccacgag ggggacggtg cgatagccgg ccgcgtgcat gcggttgatc gcgaagcaga
     5881 tccgatcgtc gggcgccagc gcttccgggt cgggcgtcat cacgtcgccg attcgcgtcg
     5941 ccgccaggtt gcgtcccagg ctgacgacgc gggtcagcag gtcgcgctcg gtgaagatgc
     6001 cggtgagccg gccggcggcg tccacgatga ccaccgcggc ccgccggttg tcgaccatgc
     6061 gggcgaccgc ttcggcgacc gtcgtatccg ggctgacgcg aatcggctcg ctgggagcga
     6121 gcagacgcac ctggtcgctc agcagcgcgt cttcgagctt ccggaactca ccgtcgaggg
     6181 cttccgagta ctcgtcgtgg aactcgctca tcgccgccac ctcaaggatg acagccatgc
     6241 taggaccagg tcctggtgct gtcaaggctg tgcgctcggt cctgtgggaa ggtgcttacc
     6301 tactcagggt ggttcagcgg ccaagctcgg tccggctctt cccaccagat gctgcgcact
     6361 cggcgacgcc ccctgcaggt cggttcgatc cgacgatggt cgaatgtaat gtgatctatt
     6421 tccgactact cactaaagtg cttcgacgac ggacgtaatg tatctgctgt ccgtcaagat
     6481 tgcgagcgcg cgacggagtg atttccacgc ccgcgaccgg gccgtcgagc tgcccatcgt
     6541 ggcaccgagt ttgcccggta tcgagcgcct tgaaagtcat ttgcccgcgg tgtcggcagg
     6601 aggggaagcc ttgcctcctg gtcacgattg atattccggg ggacgaccgc gaagtgcacg
     6661 ggctctgctg gacccaccgg ctgcgcctcc tcgcccggag tgaggatccc gcgacgcccg
     6721 accccgggcc gcgctgagcc atgttgctga ccggtcgatt ccgacgaatc tcactgtggc
     6781 tctggagcac cccgtcgcgc gggcccatgc aggtgttcgt gcgccccgac cggcccgcgc
     6841 tggtggagcg catgcagcgg ctcttcgcgg aggactccgg cgaggtcgag gtgatgctgg
     6901 accgtcggcg ccgcgagcgg cggagcgcgg cggcggcgta tgagccggag cgccggcgcg
     6961 cggatcggcg ccggccgcgc gtcccccggc gggttccggg cgcagcggtg gcgatcggcc
     7021 acacgtcgcg gcccgggctg gactacactc cactcaatcg tacccaagga gaggatcgga
     7081 tgatgagcgt cgagtccaag caggtgcgcg atggcgacga agtgcggcgc tggctcgagg
     7141 ccgggcaaac gcagctggcc tcgctgctgg aattgctgca cgagcacgat cgccttcgcg
     7201 agcgggtgga ggcctccgag cgcgagaacg agcggctgcg cggcgtgacc tacgagaacg
     7261 agcagctgcg caaccggctc gagacctccg agcgccaggc ggagcacctg cgccagagca
     7321 tgagcgagct gcggggggag aacgagcggc accagaagga gcgggaagac gcggcggagc
     7381 ggctcaacca cctggtcaac gagatcgcgc aacggctgcg cccgggcggc gggacaccgc
     7441 gctcctgagc cggcgaccgg ggccgtcgac gctcggcgcc tccgtccggt gagggtcgcg
     7501 acgccaattt ttgtccgctc tctggcgcgc ggtgcgcgtc agggagcggg catgcgcgcg
     7561 agttcagcgg ttttgcgagc gggttcccgc tggcactcgc gttgcagcca tcaccaggct
     7621 ggtggccccg cgcagaactt tctctatgag cgaacccgtg atcgagaccg aagagaagcc
     7681 tcgcgtgctc ctcgtggacg acgaggaggg agtacgcatg agcttgcgcg ccttgctcga
     7741 tcgcgagtac cggatctcga gcgtggcgag cggcgaggcg gcggtcgagg agctgccgcg
     7801 cttccgcccc gacctggtgc tcctcgacgt gaagctcctg cccagcatga gcgggctcga
     7861 taccctccag cacatcaaga gcttcgacga gaggatcgag gtcgtgatga tcaccgcctt
     7921 cgcctccctc gtcaccctca acgaggcctt gcgtcgcggg gcgttcggct atctgatcaa
     7981 gcccttcccg cgccgggatc tggccctcat ggtgcgcgac gccctggccc ggcgcaacgg
     8041 ccgggtcgac ggggcgtgac cgaatatatc cggtcgttga cagaagcgcg cggcgcggtg
     8101 agaatttccc ggtacgtact gtcaccggga cgcgggcccg cccgaatccg aacgtcgtgt
     8161 ctctcaccgg gaggacgtca tccatgagtc tcacgagtca agtcgagaag tgggtggatg
     8221 aggtcgccgc gctgaccggc ccggcgaaga tcgcctgggc cgacgggtcg aaggcggagt
     8281 acgaccgcct ggtcgacgac atgatgcggg acggcaccct gcttcccttg aactcgcgga
     8341 cctatccgaa ctgctacctg catcgcagcc acccctccga cgtggcgcgc accgagcagc
     8401 tgaccttcat ctgcacgccc acgcgcgagg aggccgggcc caccaacaac tggatggctc
     8461 ccgcggaggg caaggagcgg ctcaaggggc tgttccgcgg cagcatgcgc ggccgcccga
     8521 tgtgggtgat cccgtacctc atggggcccg ccggctcgtc gatgagccgc gtcggggtga
     8581 tggtcaccga cagcgcctac gtggtggcca gcatgcacat catgacgcgc gtgggagcgg
     8641 tggcgctgga gcacatgcgc gacggcgacg acttcatccg gggcctgcac tcgctcggcg
     8701 acctctcgcc cgaccgccgc ttcatcatgc attttccgga ggagcggctg atctggagcg
     8761 tggggtcggg ctacgggggc aacgcgctgc tcggcaagaa gtgccacgcc ctccgcatcg
     8821 cgtctcacca ggcccgcacg gaaggatgga tggccgagca catgctgatc ctcggcctcg
     8881 aggacccgga ggggcggatc acctacctcg cggccgccat gccgagcgcc tccggcaaga
     8941 ccaacctcgc gatggtggtg tcgagcctgc cgggctaccg ggtgtggacg gtcggcgacg
     9001 acatcgcctg gatccacgtc gacaagcacg gtcagctgcg cgccacgaat ccggagcgcg
     9061 gcttcttcgg ggtggcaccc aacaccagcg ccaagacgaa tccgaacggc gcggccatgg
     9121 tgcgctcgaa caccatcttc accaacgtgg ccctcacccc agccaacgag ccgtggtggg
     9181 aggggatggg ccccgagccg cccgccggcc tgctcgactg gcagggcaag ccgtggaccc
     9241 cgggatcggg cccggccgcg catcccaact cgcgattcac ggtgccggcg cagcagtgtc
     9301 cctccatcgc cccgaactgg gaggaccccg agggcgtgcc gctctccggg ttcatcttcg
     9361 gctcgcgtcg cgcgcgggtg gtgccgctgg tcttcgaggc gttcgactgg gatcacgggg
     9421 tcttcctcgg ctcagcgatg ggtaccgaga cgaccgcggc catcaccggc caggtcggag
     9481 tggtgcggcg tgatccgatg gccatgatcc cgttctgcgg ctacaacatg gccgactact
     9541 tcgcgcactg gctgtccttc ggtccgaagc tcaagaaccc gcccaagatc ttccgggtga
     9601 actggttccg gcgcgacggc gacggccggt tcctgtggcc gggctacggc gagaacgtgc
     9661 ggatcctcaa gtggatcgtc gaacggatcc agggcggcgg gaaggcggtc gagacgccga
     9721 tcgggcacgt gccggcgccg ggaagtctcg acgagacggg gctgtcgctg gcccccggcg
     9781 cgctcgacgc cgccctgcac gtcgaccgcg aggagtggcg tcaggcgctc gacgagctgc
     9841 gcgagttcta cgagcagttc ggcgagcgca tgccgcgcgc gatctggaag gcgcacgccg
     9901 agacggcgcg gcgcttcggg ctctgatccg aacgtggcga gtcacatgag tcgaggcggc
     9961 gcgcgacggg tcctcctcgg gctcggactg gtggtggccg gactctccgg gtgtgcgagc
    10021 agcgacgcca gcttcccgcc tccgcagccg cccgccgcag cgcagcactt caaggcgacc
    10081 gcacccggcc agtttccgat cgacgtgtac ttcacggcct ggggtgaggg ctacgtgatc
    10141 tatgcgcccg gtcaggcgcc gatctacctc atctccgaca agcagggcgg gttcatcatc
    10201 cagcgtcccg gcgagtccgc cgccttcgtc acgcgacgaa aggacgccag cgggtggaac
    10261 atcctcaggg ccaatgcgcc ggcgaccatg ttcctgaaga acccggaagg cgaaggctgg
    10321 atcctccaac cccccggtga gctgccgacc ctgatcgtgc ccctgcctca gtaacgcact
    10381 gtctcccagc tgacactcgt tcgtcccgcc aacgtaccgc gcgagcggcc tgtctccagc
    10441 cacgaccagg atacggcttt gttatctgtc agttaactgg tccctccccg tggcagggtg
    10501 attgctctcc tgtggatcgc tttccacaac gccagcagcc accaggaggc cgcgatgttc
    10561 ggatgcaaag gtgccacggg cctcctcgcc cggcctccga ggaccgaggc gcatcgcctc
    10621 ggggcgcagg cgtagcggtc ccatgtggcc ctggagtcgc ccctccgccg ccgccagccg
    10681 caagaagctg accgccttcc tcgatgaggg ttccgacatc gagggcaagt acaccttcac
    10741 cggcaccgtc ctcctcaacg ggaagttcca gggcgagatc gcctcgcccg acacgctgat
    10801 catcggagag cgcggcgtgg tgaacgcgag tgtccgggcg ggcacgctcg tggtcaacgg
    10861 cgagctggtc ggcaacatct acgcgagtga gcgcgtggag ctcacccgga aggcccgcgt
    10921 ccacggtgac atcgaatccc cgatcctcgt gctcgaggaa gggggcacgt tcgagggccg
    10981 ctgccggatg cccaggccgg acgcgatggg ctcggctcgt gacgcgaccg taatgccgct
    11041 caagcgctca tgagccagcc gcgcgggggc ttcactctgg tcctggtgca cggcggcggc
    11101 agccgcatgc tccacgtggg ttgcccgcgc tggctcctct gggggatgtt catcgcggtg
    11161 accgcgttcg tggccctcct caccgggacc tggtgggact acttcgcgct gaagcgggcc
    11221 cagatccagg cggccagcgt cgagcgccgg gtcgaggagc agcgggtgat gctcgactcg
    11281 gtcgagcagc gagtggccga tctcaaccgc gaggtcggag gatggcaggc ggcgcacctg
    11341 cgggtgtgga gcacgttcgg gccggctccc ggaagcagcg tcaaggggac gggcatgggc
    11401 ggtgcgatgg cggtgctccc gctcgtcttc gccaatgagg cgccggccct gccacaggcg
    11461 ctggcgctcc tgtcgagcag cgtgggcgag agcgggcaga gcctgcgcgc gctcgggcgc
    11521 ttcatggagc gtacccgccc catgctcctg tcgctgccgc tgcgctggcc ggtgcgcggg
    11581 ccgctcaact cgcggttcgg catgcgccag tccccgtggt ccggcgagca agagttccac
    11641 cgcggcctcg acatcagcgc gaatcggggg accgcggtcc tggccccggc ctccggcacc
    11701 gtgtacttcg cgggcagcag cggcgagtac ggcaccaccg ttatcctcga tcacggccac
    11761 gagctgcgct cgctctacgg acacctgcag gagatccgcg tgaagccggg cgagcgcatc
    11821 gaccggggac aggtcatcgc cctcaccgga agcaccggcc gcacctccgg cccgcacctg
    11881 cactacgaga tccaggtcca cggtcaggcg gtcgaccctc gccagttcct ctgggactag
    11941 cggagccgct tcctcctccg gggcctcgcc ccggccccgc agttgtgctg tgatcctcct
    12001 cactcgacgt c
//
LOCUS       DATMCV010000137         3377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_141533, whole genome shotgun sequence.
ACCESSION   DATMCV010000137 DATMCV010000000
VERSION     DATMCV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141533"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VNU03_03345"
     CDS             complement(<1..272)
                     /locus_tag="VNU03_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HWP73514.1"
                     /translation="MASETKGSSAVEALLKEKRKFAPPKEFVKRATANRATVYSEAAK
                     NPARFWERFARELDWFKPWKTTLQWKAPNAKWFVGGKLNVAYNCLDR"
     gene            368..1603
                     /locus_tag="VNU03_03350"
     CDS             368..1603
                     /locus_tag="VNU03_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HWP73515.1"
                     /translation="MPIPLPLYSVLPFAAMLLAIALLPLALPHWWESNRNKLLVSAGL
                     GLPVLLLYLARAPQVLAHTASDYASFILLLAGLYVIAGGIRLRGDLEATPLVNSAFLA
                     AGAALASFIGTTGASMLIIRALLQTNQERERVTHTVVFFIFLVSNIGGMLTPLGDPPL
                     FLGYLAGVPFAWTLRLWRPWLTMILVLLVVYFVWDTIQHRRESRAALRRDHTQIEPLR
                     IDGGLNGLWLVGVVAAVAVLHAPLRELAILGLAGISLWCTPRPLRRANQFTAQPMVEV
                     AVLFLGIFLTMIPALELLHARGGELGVREPWQFFWAVGGLSSLLDNAPTYVAFLALGQ
                     GLGLPGEVVGVPHAMLAAISVGAVAMGANTYIGNAPNFMVKAIAEAAGVRMPSFLGYT
                     IYSGAVLMPLFVAVTLLFF"
     gene            complement(1600..2988)
                     /locus_tag="VNU03_03355"
     CDS             complement(1600..2988)
                     /locus_tag="VNU03_03355"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP73516.1"
                     /translation="MKRRLLLVEDDEVFLRPLLRTLELKGYEVLPVQSGEEALEAVKA
                     EDVDLVLTDRRLPGMDGVELVRQIKTEHPDLAVVVMTAYGTIESAVEAMRLGAEDYLV
                     KPFEAAELLLVIRRAIEFQELKSANRRTIRRNQERFTFGNIVAASAGMQGVFDLLRSV
                     VDLDTTVLIYGETGTGKELLARSIHFSGARRERPFVAVNCAAIPQELFESELFGSRRG
                     AFTGATEHRRGHFQMANSGTLLLDEIGEMPLHLQSKLLRAIEERKVTSVGADRPVDID
                     VRFIATTNKDLQAEVERGTFRRDLFYRLSVMPVRVPPLRERPEDIPLLARHFVEQAGR
                     RLKKAVRGISPESLGALTRYPWPGNVRELENVIERAAIVAVGDTLADVERFLAPTGAH
                     APVDLSLPFRDAKARVVEDFERAYIEGLLKSHGGKLTIAAKHADMDPKNFSDKMQRYG
                     LRRATTPEPPPV"
     gene            complement(2985..>3377)
                     /locus_tag="VNU03_03360"
     CDS             complement(2985..>3377)
                     /locus_tag="VNU03_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP73517.1"
                     /translation="GRLDREVTAAVDGQKLRQVFTNVMKNALEAIEPRRQTGRVTVDL
                     FVEGERAVVEVADDGVGISPEDRDRIFLPFFTTKPSGTGLGMSIVKKIVDLHGGDIVI
                     ESAPGRGTRVRISLPAVLAPQPLAGGTS"
BASE COUNT          437 a         1231 c         1129 g          580 t
ORIGIN      
        1 cggtcgaggc agttgtaggc gacgttgagc ttgcccccga cgaaccactt ggcgttgggc
       61 gccttccact ggagcgtcgt cttccacggc ttgaaccagt ccagctcgcg cgcgaagcgc
      121 tcccagaagc gggccggatt cttcgccgcc tccgaataga cggtggctcg gttggcggtc
      181 gcgcgcttga cgaactcctt cgggggagcg aacttccgct tttccttcag aagggcttcg
      241 acggcgctgc tgcctttggt ctcgctggcc attgaggaac ctcgtcgtgg ggtgagatcg
      301 gcgcctcggc gcgccgcctc ccttctaaac catccgtcgg tgaacttcaa gagagtaagc
      361 tcgccccgtg ccgattccgc tcccgctcta ttccgtgctg cccttcgcgg cgatgctcct
      421 cgccatcgcg ctcctgcctc tcgcgctgcc gcactggtgg gagtccaacc ggaacaagct
      481 gctcgtgtcg gccggtctcg gcctgccggt gctgctcctc tacctggcgc gcgcgcccca
      541 ggtcctcgcg cacaccgcga gcgactacgc gtcgttcatc ctcctgctgg ccggcctcta
      601 cgtgatcgcg ggcgggatcc ggctccgggg cgacctcgag gccacgccgc tggtcaacag
      661 cgccttcctg gccgcgggcg ccgccctcgc ctcgttcatc ggcaccaccg gggcctccat
      721 gctgatcatc cgcgccctcc tgcagacgaa ccaggagcgc gagcgcgtga cccacaccgt
      781 ggtgttcttc atcttcctgg tctccaacat cggcgggatg ctcacgccgc tcggcgaccc
      841 gccgctcttt ctcggctatc tggcgggggt gccgttcgcc tggaccctgc gcctgtggcg
      901 gccctggctg accatgatcc tggtcctcct cgtcgtctac ttcgtctggg acacgatcca
      961 gcaccgccgc gagtcccgcg cggcgctccg gcgcgatcac acccagatcg agcccctgcg
     1021 catcgacggg ggcctcaacg gcctctggct ggtcggcgtg gtcgccgcgg tggcggtgct
     1081 gcacgcgccc ctgcgcgagc tcgccatcct ggggctggcc gggatctccc tctggtgcac
     1141 gccccggccg ctccgccggg ccaaccagtt caccgcgcag ccgatggtgg aagtggcggt
     1201 gctcttcctc ggcatcttcc tgaccatgat ccccgcgctc gagctgctcc acgcgcgcgg
     1261 cggcgagctg ggggtccgcg agccgtggca gttcttctgg gcggtcggcg ggctctcgtc
     1321 gctcctcgac aacgcgccca cctacgtcgc gttcctcgcc ctgggccagg gactcggcct
     1381 gcccggggag gtggtcggcg tgccccacgc gatgctggcc gcgatcagcg tgggcgcggt
     1441 cgcgatgggc gccaacacct acatcggcaa cgcgcccaac ttcatggtga aggccatcgc
     1501 ggaggcggcg ggggtgcgca tgccgagctt cctcggctac acgatctaca gcggcgcggt
     1561 gctgatgccg ctcttcgtcg cggtcaccct gctgttcttt taaacgggcg ggggctccgg
     1621 cgtcgtggcg cggcgcagac cgtagcgctg catcttgtcc gagaagttct tggggtccat
     1681 gtcggcgtgc ttggccgcga tggtgagctt gccgccgtgg ctcttgagca gcccctcgat
     1741 gtaggcccgc tcgaagtcct ccactacccg ggccttggcg tcccggaagg gcagcgagag
     1801 gtccaccggg gcgtgggccc cggtgggggc caggaaccgc tccacgtccg cgagggtgtc
     1861 gccgacggcc acgatggccg cgcgctcgat gacgttctcg agctcccgca cgttgcccgg
     1921 ccacggatag cgtgtcaggg cacccaggct ctccggcgag atcccgcgca ccgccttctt
     1981 caggcggcgg ccggcctgct ccacgaagtg ccgggccagc agcgggatgt cctccggccg
     2041 ctcgcgcagg gggggcacgc ggaccggcat caccgagagc cggtagaaga ggtcgcgacg
     2101 gaaggtgccg cgctcgacct cggcctggag gtccttgttg gtggtggcga tgaagcgcac
     2161 gtcgatgtcg accggccggt cggcgcccac cgaggtgacc ttcctctcct cgatggcccg
     2221 caggagcttg ctctgcaggt ggagcggcat ctccccgatc tcgtcgagca ggagggtgcc
     2281 gctgttggcc atctggaagt gcccccgccg gtgctcggtg gccccggtga aggcgccgcg
     2341 gcgcgagccg aacagctcgg actcgaagag ctcctgcggg atggccgcgc agttgaccgc
     2401 cacgaagggc cgctcgcgcc gggcccccga gaagtggatc gagcgggcca gcagctcctt
     2461 gccggtgccg gtctccccgt agatgagcac cgtcgtgtcc aggtccacca ccgaccggag
     2521 caggtcgaac accccctgca tcccggcgct ggcggccacg atgttgccga acgtgaaccg
     2581 ctcctggttg cgccggatcg tccgccggtt cgccgacttc agctcctgga actcgatcgc
     2641 gcgccggatg accaggagca gctcggcggc ctcgaacggc ttcaccaggt agtcctccgc
     2701 gccgaggcgc atcgcctcca ccgcggactc gatcgtcccg taggcggtca tgaccaccac
     2761 cgccagatcg gggtgctcgg tcttgatctg ccggaccagc tcgaccccgt ccatgccggg
     2821 cagccggcga tcggtgagca cgaggtccac gtcctccgcc ttgaccgcct cgagcgcctc
     2881 ctccccgctc tggaccggca gcacctcgta gcccttcagc tcgagggtgc ggagcagcgg
     2941 acgcaggaag acctcgtcgt cctcgaccag caagagccgt cgcttcatga tgtgcctccg
     3001 gccagcggct ggggcgcgag caccgccggc agtgagatgc ggacgcgcgt gccgcggccc
     3061 ggtgcgctct cgatcacgat gtctcccccg tggaggtcca cgatcttctt gacgatcgac
     3121 atgccgaggc cggtgcccga cggcttggtg gtgaagaagg gcaggaagat ccggtcgcgg
     3181 tcctcggggg agatacccac cccgtcgtcg gcgacctcca ccaccgcccg ctcaccctcg
     3241 acgaagaggt cgacggtcac ccgcccggtc tgccggcgcg gctcgatcgc ctccagcgcg
     3301 ttcttcatca cgttggtgaa gacctggcgg agcttctgac cgtccaccgc cgcggtgacc
     3361 tcgcgatcga ggcgtcc
//
LOCUS       DATMCV010000138         5649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_339195, whole genome shotgun sequence.
ACCESSION   DATMCV010000138 DATMCV010000000
VERSION     DATMCV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339195"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VNU03_03365"
     CDS             complement(<1..513)
                     /locus_tag="VNU03_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWP73518.1"
                     /translation="MATRWRSVGVVVTPGSGEGRARGVARRLARLLRRRGSTVTIRAF
                     GGLPALAEWAETAAAEFSVIFCIGGDATQSAAAALARRHRIPFVPVPTGFGNLFASVF
                     GHPASARRAARLLADGEIRMVDVGLAGDEPFLSHKSYGFIDQVQERVEEDRRQPRTRA
                     CRLLAYYGIAA"
     gene            complement(525..992)
                     /locus_tag="VNU03_03370"
     CDS             complement(525..992)
                     /locus_tag="VNU03_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73519.1"
                     /translation="MPFVEMSGEMSGEKGRAWIWAALIVEIIGLSIDAVWHGLLGSEV
                     EPQTRGEMARHLATVHLVLYAGVVMLFLATAWAVRHAARRRLLFLAFAGAAIQLVGEV
                     WHAFSHLQLRPNPFPELAGFVGLAVAIVATVVAGRSVPAAMTGRRLERSPQGR"
     gene            1227..1415
                     /locus_tag="VNU03_03375"
     CDS             1227..1415
                     /locus_tag="VNU03_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73520.1"
                     /translation="MNAAVMIDTCFRHQHPEAYGEIEVPEPLVPVQLRYGHDRSPVPS
                     LECLAEESLGYPDWRALT"
     gene            1468..3090
                     /locus_tag="VNU03_03380"
     CDS             1468..3090
                     /locus_tag="VNU03_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006007312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HWP73521.1"
                     /translation="MTETRFPEARAPRGMVATPHLLATQSGVAALRAGGNALDAAIAA
                     AATIAVVYPHMNGLGGDNVWLIHDARNQSLRALCGVGRAARVATIDWYAARGITQALP
                     VRGGPAALTVPAVVDGWWQAHRHSRDAMGSPLDWKALLADAVAYAREGFTASDGQRIP
                     PPREPDLFTDRAPEMIRRWLWPLYHPDALRRGPLVQRDLARTLEAVRDGGAEAFYRGD
                     VARRLVTAAAAAGSPLALEDLAEHRSEWMEPLRIPYAEGHAVSVPPPTQGLSALAVLG
                     MMERFDVASLPEADYVHLAVEATKLAFEDRDRYLTDPAHMRVAAAELLGTERLKARAR
                     LISPAHVRPSQPAAAAGGDTIALVTADREGNAVSLIQSLYFTFGAGLMAGDTGVVLQN
                     RGSFFSLDPGHVNALAPGKRTMHTLIPSMYLEGGRPRFVYGTMGGEGQPQTQAAILTR
                     RLFRRLGPQAAVEAPRWLYGRTWGTATRALSLEGRYPAELARELERRGHEVAIGEDWD
                     DLFGHAHCIWVAPEGGLVGGSDPRADGGALGY"
     gene            complement(3087..4694)
                     /locus_tag="VNU03_03385"
     CDS             complement(3087..4694)
                     /locus_tag="VNU03_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWP73522.1"
                     /translation="MILRLVGFVVAVIMTTAGLPALVLAQKPLTVQEALLRAKPAAVL
                     VVAEVSSEVTLDCGAGPVKITPPVFRETGTGWFLDADGWLMTNGHVVQPAYEPPRWLA
                     NQQAQRAVTTACLPPVLKKMGLQPGDRPDTEEAAKRRLLDTVLPSAKVNLKPDVFVVT
                     AKGRFKAEVKKYSPPVNEMSGRDLALLKVPGENYPVLSLADSKAVQIGDPVHIIGFPG
                     VVLTHELLNQSSMVEASITNGAVSGFKQDKNNNTVIQTDASAAWGNSGGPVVDDRGNV
                     VGLLTFVSLAPGPEGSIVQGFNFVIPSAAVTDFVKGTAVKVNAGSKFNDAWFAGLSAL
                     FSEDGPSALRHFEEADRLHPNLPDARRMLAEARDAVKNPKPKPFPWFWVAIGVTFVSA
                     GGFGGQWLHRWHKNRYRVHPSEVVRLIEGGKAPTIIDARRTDLYEQLPLMIRGSVRVA
                     PENLAASMAGLDLDPNRPVVAYCSCPDEETSARVAKELRKMGFKDVKILKGGLGAWTN
                     AGLPIETRSDLSQVGVELYKALAGGNV"
     gene            4735..5127
                     /locus_tag="VNU03_03390"
     CDS             4735..5127
                     /locus_tag="VNU03_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWP73523.1"
                     /translation="MSRQIGEVPTRTFEPGAVIFREGDDAKGEAFMVHQGRVEIRKQI
                     GGEERLLRALTKGELLGHIALFRNAPRSASAIAADAVTLMVISANRLDHLVRANPALA
                     LALIRDLSTMMLAAEDRLREVEDRLGKK"
     gene            complement(5134..>5649)
                     /locus_tag="VNU03_03395"
     CDS             complement(5134..>5649)
                     /locus_tag="VNU03_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729870.1"
                     /note="catalyzed the formation of 2-ketoglutarate from
                     2-hydroxyglutarate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HWP73524.1"
                     /translation="ERRSIVRGLIYPVPDPDFPFLGVHFTRTIHGDVEAGPNAVLAFA
                     REGYSLGTVRPGEFLGTLGYAGFWHMARKYWRVGAYEMYRSASKAAFVRSLQKLVPDI
                     RSGDLERGGAGVRAQAVAPDGSLVDDFKISVTPGAVHVVNAPSPAATASLAIGRYVAG
                     VAAETFGLGAA"
BASE COUNT          821 a         2076 c         1958 g          794 t
ORIGIN      
        1 cgccgcgatc ccgtagtagg ccaggagccg gcacgcgcgt gttcgcggct ggcgtcgatc
       61 ctcctccaca cgctcctgga cctgatcgat gaagccgtag ctcttgtgag agagaaacgg
      121 ctcgtcgccc gccaggccca cgtccaccat ccggatctcg ccgtccgcca gcaatcgtgc
      181 ggcccggcgc gccgacgccg ggtggccgaa cacgctcgcg aacaggttcc cgaatccggt
      241 cgggaccggc acgaacggga tccggtggcg ccgggccagc gcggcggccg cactctgagt
      301 ggcgtctccg ccgatgcaaa agatcacgga gaactccgcg gccgctgtct ccgcccactc
      361 ggcgagcgcg ggcaggcctc cgaatgcccg gatcgtgacc gtggagcccc gccgccgcag
      421 cagccgtgcg aggcggcgtg ccacgccgcg ggcgcgcccc tcgccggaac ccggcgtcac
      481 cacgaccccg accgaccgcc accgggtggc catgcgggtc gggctcagcg gccctgcggg
      541 ctccgctcca gccggcgccc ggtcatcgcc gccggcacgc tgcgaccggc gacgacggtc
      601 gcgacgatcg ccaccgccag cccgacgaag ccggccagct ccgggaacgg gttcggccgg
      661 agctggaggt gcgaaaacgc gtgccacacc tctcccacca gctggatcgc tgcccccgcg
      721 aaggcaagga agagcaggcg gcgccgggcc gcatgccgga ccgcccatgc ggtggcgagg
      781 aagagcatca cgacccccgc gtagagcacg agatgcacgg tcgccagatg cctcgccatc
      841 tcaccccggg tctggggctc gacctccgac ccgaggagcc catgccagac cgcgtcgatg
      901 ctcaagccga tgatctcgac gatcagcgcc gcccagatcc aggcccgccc cttctccccg
      961 ctcatctccc cgctcatctc aacgaagggc aatcgctccc tcctccctcg aaggcgtatc
     1021 ggttcgtcgg gagactccga gcaaaccgtg tgccgcccgc gttccctcca tcgcgcccca
     1081 tcaatctggc tttcgcgatg gcgggcaacc gacgtcgacc aacctttgcc cggtcaactt
     1141 taccgatgcc tccaggaatt tccgcccggc ggcagtggcg cagctcttgc tctcctggtg
     1201 cgcggaggaa caggaggaac ccgatcatga acgcggcggt gatgatcgac acgtgcttca
     1261 ggcatcagca tcccgaggcg tacggcgaga tcgaggtacc ggagccgctc gtccccgtgc
     1321 agctacgata cggtcacgat cgctccccgg tgccctcact ggagtgcctg gccgaggagt
     1381 cgctcggcta cccggactgg agggcgctga cctagggggg acagatcgtg cgcagagccg
     1441 ctcgaggagc cgtgctaaag atacgtcatg acggagaccc ggttccccga ggcgcgcgcc
     1501 cctcgcggca tggtggccac tccgcacctg ctcgcgaccc agtccggcgt ggccgccttg
     1561 cgcgcgggag gcaatgcgct ggacgcggcc atcgcggccg ccgccaccat cgcggtggtg
     1621 tatccgcaca tgaacggcct gggcggcgac aacgtgtggc tgatccacga cgcgcggaac
     1681 cagagcctgc gcgccctctg tggcgtgggc cgggccgcgc gggtcgccac catcgactgg
     1741 tacgccgcgc gcggcatcac gcaggccctg ccggtgcgcg ggggacccgc ggcgctcacg
     1801 gtccccgcgg tggtcgacgg ctggtggcag gcccaccgcc acagccgcga cgcgatgggc
     1861 tccccgctcg actggaaggc cctcctcgcc gatgcggtgg cctacgcgcg cgagggcttc
     1921 accgcctcgg acggccagcg catcccgccg ccgcgcgagc cggatctctt caccgaccgc
     1981 gcgccggaga tgatccggcg gtggctctgg cccctctacc atccggacgc cttgcgccgc
     2041 ggcccgctgg tgcagcgcga cctcgcccgc accctcgagg cggtgcgcga cgggggcgcg
     2101 gaggcgttct accgcggcga cgtggcccgc cgcctggtga ccgccgccgc ggccgcgggc
     2161 agcccgctgg cgctcgagga cctggccgag caccggtccg agtggatgga gccgctgcgc
     2221 atcccctacg ccgagggaca cgcggtgagc gttcccccgc ccacccaggg gctctccgcg
     2281 ctcgcggtgc tcgggatgat ggagcggttc gacgtcgcct cgctgccgga ggccgactac
     2341 gtccacctcg cggtggaggc caccaagctt gcgttcgagg atcgcgaccg ctacctgacc
     2401 gacccggccc acatgcgcgt ggccgccgcc gagttgctcg gcaccgagcg tctgaaggcg
     2461 cgcgcccggc tgatctcacc cgcgcacgtg cgcccatccc agcccgccgc ggccgcgggc
     2521 ggcgacacca tcgcgctggt caccgcggac cgcgagggca acgcggtctc cctgatccag
     2581 tcgctctact tcacgttcgg cgcgggcctc atggcaggcg acaccggggt cgtgctccag
     2641 aatcgcggct cgttcttctc cctggacccg ggccacgtca acgcgctcgc gcccggcaag
     2701 cgcaccatgc acaccctgat cccgtcgatg tatctcgagg gcggtcgccc gcgcttcgtc
     2761 tacggcacga tgggcggcga gggccagccg cagacccagg ccgcgatcct cacccggcgg
     2821 ctcttccgcc ggctcggccc tcaggccgcc gtcgaggctc cgcgctggct ctacgggcgc
     2881 acctggggca cggccacccg cgcgctcagc ctcgagggcc gctacccggc cgagctcgcg
     2941 cgtgagctcg agcgccgcgg gcacgaggtc gcaatcgggg aggactggga cgatctcttc
     3001 ggtcacgccc actgcatctg ggtcgcgccg gagggcgggc tggtcggcgg gtcggacccg
     3061 cgggcggacg gcggggcgct ggggtactag acgttgccgc cggcgagcgc cttgtacagc
     3121 tcgacgccca cctgcgagag atcggagcgg gtctcgatgg gcaggcccgc gttggtccag
     3181 gccccgagac cgcccttcag gatcttcacg tccttgaacc ccatcttgcg cagctctttc
     3241 gccacccggg cgctggtctc ctcgtcgggg cagctgcagt aggcgaccac cggccggttc
     3301 gggtccagat ccaggccggc catgctcgcg gcgaggttct cgggagccac ccgtaccgag
     3361 ccgcggatca tgagcggcag ctgctcgtag agatccgttc gccgggcgtc gatgatggtg
     3421 ggcgccttgc cgccctcgat cagccgcacg acctccgagg ggtgcacgcg gtagcggttc
     3481 ttgtgccagc ggtggagcca ctgcccgccg aagccccccg cgctcacgaa cgtgactccg
     3541 atggccaccc agaaccacgg gaagggcttg ggcttcggat tcttcaccgc gtctcgcgcc
     3601 tcggccagca tgcgccgggc gtccggcagg ttcgggtgca ggcggtcggc ttcctcgaag
     3661 tgccgcagcg cgctggggcc gtcctcgctg aacagggcgc tgagcccggc gaaccaggcg
     3721 tcgttgaact tgctgcccgc gttcaccttc accgcggtgc ccttcacgaa gtcggtgacc
     3781 gcggcggacg ggatgacgaa gttgaagccc tgcacgatgc tgccctcggg ccccggcgcc
     3841 agcgacacga acgtgaggag cccgaccacg ttgccccggt cgtcgaccac cggtccgccc
     3901 gagttgcccc acgccgccga ggcatcggtc tggatcacgg tgttgttgtt cttgtcctgc
     3961 ttgaagcccg acaccgcgcc gttcgtgatc gacgcctcga ccatggagga ctggttgagc
     4021 agctcgtgcg tgagcaccac gcccgggaag ccgatgatgt ggaccgggtc gccgatctgg
     4081 actgccttgg agtcggcgag cgagagcacc gggtagttct cgcccgggac cttgagcagg
     4141 gcgagatcgc gccccgacat ctcgttcacc ggcgggctgt acttcttcac ctcggccttg
     4201 aaccgtccct tggcagtgac gacgaagaca tcgggcttga ggttgacctt ggcggagggc
     4261 agcacggtgt cgagcaggcg acgcttggcc gcctcttcgg tgtccggccg gtcgcccggc
     4321 tgaaggccca tcttcttgag caccgggggc aggcaggccg tcgtgaccgc ccgctgggcc
     4381 tgctggttgg ccagccagcg gggcggctcg taggcgggct ggaccacgtg gccgttggtc
     4441 atcagccagc cgtcggcgtc gaggaaccag ccggtcccgg tctcccggaa gaccggcggg
     4501 gtgatcttga ccggcccggc gccacagtcg agggtgacct cggaggagac ctcggccacg
     4561 accagcacgg cggccggctt ggcgcggagg agggcttcct ggaccgtgag gggtttctgg
     4621 gcgagcacga gggcgggcaa cccggcggtg gtcatgatca ccgccaccac gaaccccacc
     4681 agacgcagta tcataggcgc actgcgggcc attctacacc gaagacagga gttcatgagc
     4741 cgccagatcg gagaggtacc gacgaggacg ttcgagccgg gcgcggtcat cttccgcgag
     4801 ggagacgacg ccaagggcga ggccttcatg gtgcatcagg gccgcgtgga gatccgcaag
     4861 cagatcggcg gcgaggagcg gctcctgcgc gcgctcacca agggcgagct gctcgggcac
     4921 atcgcgctct tccggaacgc gccccgctcg gccagcgcga tcgcggccga cgcggtcacc
     4981 ctgatggtca tctccgccaa ccggctcgat catctggtgc gggccaaccc cgcgctcgcc
     5041 ctcgccctga tccgcgacct gtccacgatg atgctggccg cggaggaccg cctgcgcgag
     5101 gtggaggatc ggctggggaa gaagtagggc gcctcaggcg gcgcccagcc caaacgtctc
     5161 ggcggccacg ccggcgacgt accggccgat ggcgagcgag gcagtcgcgg cgggagacgg
     5221 ggcgttgacc acgtgcaccg cacccggcgt cacgctgatc ttgaagtcgt ccaccaggct
     5281 cccgtcgggc gccaccgcct gcgcgcgcac ccccgccccg ccccgctcga ggtcgccgct
     5341 ccggatgtcc ggcaccagct tctgcagcga gcgcacgaag gcggccttgc tcgccgagcg
     5401 gtacatctcg taggcgccga cgcgccagta cttgcgcgcc atgtgccaga agccggcgta
     5461 gcccagggtg ccgagaaatt cgcccggccg caccgtgccg agcgagtacc cctcgcgagc
     5521 gaaggcgagc accgcgttcg gtccggcctc cacgtcgccg tggatggtgc gggtgaagtg
     5581 cacgcccagg aacgggaagt cggggtccgg caccgggtag atcaggccgc gcacgatcga
     5641 tcgccgctc
//
LOCUS       DATMCV010000139         5114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_206137, whole genome shotgun sequence.
ACCESSION   DATMCV010000139 DATMCV010000000
VERSION     DATMCV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206137"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..767
                     /locus_tag="VNU03_03400"
     CDS             9..767
                     /locus_tag="VNU03_03400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP73525.1"
                     /translation="MTSTQKVAIVTGASSGIGKASALALMKEGYAVVFAGRRKDLLES
                     AAAEGKGSPGKSLVVQTDVTSHESIKALFAKTKETFGRLDVLFNNAGGGAPPVLFEDL
                     TYDQWQNVVNANLTGVFLCTQEAFKIMKAQTPMGGRIINNGSISAHAPRPNSAPYTAT
                     KHGVTGLTKVSSLDGRKYDIAVGQIDIGNAGTPMTERMAKGVPQANGTTVAEPTMNVK
                     AVADAVVYMSSLPLDANVLFLTVMATKMPFVGRG"
     gene            complement(781..2013)
                     /locus_tag="VNU03_03405"
     CDS             complement(781..2013)
                     /locus_tag="VNU03_03405"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018895245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP73526.1"
                     /translation="MNAQSVPDPEQSTAGAAGPLAGLRVLDCSSLIAGPTAAMYLGDY
                     GADVIKIEHPQGDGLRAWGAMKDGVGLYFKMLNRNKRSVTADLHTELGVEIVRRLAAR
                     ADAVIENFRPGTLERWGLGYETLAAANPGLVLTRVTGFGQTGPERDRPGFGTSAEAAS
                     GFAAINGEADGPPLLPGFALADAAAGLAGAFLTLAALQGRALRGGRGQVVDLAIYEPL
                     LTLLGPQLVEFDQLGVVQGRTGSRTPLIAPRNTYRTRDGKWIALSGASPSVFERLCRS
                     LGVPELPADPRFRDNQARLANVAALDTALQAAIGRLDQADLLARLDAAQAVAAPVRGA
                     DEVLADPHYRARGSIATVPDADLGPLRMQNAIGRLSATPGGIRHAGPRLGEHNRAVLV
                     DELGFGEAELRAGGVALL"
     gene            2106..2873
                     /locus_tag="VNU03_03410"
     CDS             2106..2873
                     /locus_tag="VNU03_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWP73527.1"
                     /translation="MLDLLEVLSSRAGGASLTELGVALGAPKTSLLPLLRTLVRRGYA
                     ARGDSGRYGVGPRWLEAGERKVAAAPDLVAAAHPVLQALTRETGETTFVAVLPPGHSD
                     LVYVDKVESPQRIRYSAELGERRPLYCTATGFGLFAFLEPAPREHLVRTLHLTRHTRR
                     TTTSRELLRRRLDAIHAAGVAVTVDEFVIGAGGAAAPVYGRDGRLVAACAVTGPTARI
                     RAGRRRLAEAAKRAAAEISARLGYHVPTGGEVKARAR"
     gene            2870..3913
                     /locus_tag="VNU03_03415"
     CDS             2870..3913
                     /locus_tag="VNU03_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73528.1"
                     /translation="MSEIDEATDPAALIEAYYEAGWTDGLPVVPPSDASIAAMLVAAG
                     LEGHEELGAITGRNAVVSAEKAAINAVMAGCRPEYMPVVVAAVRSLCAPAFAYHGPAS
                     STGGSAMALVVNGPIAPRLGINSGNNAFGQGHRPNATIGRAVRLIMMNVMNTRPGLLD
                     RATLGNPGKYSFCFAENEENHPWEPLHVARGLRREDSAVTLIASNSLYQVYNHLAADP
                     EPLLLCFADALGNVGTPNLLGFNETLIVFAGEHAEVLQKAGWSRLDVQRFLIERTRRS
                     VADYKRGARLPGAVTEGDETTWRYRFERPEDILIAFAGGRAGSWSACLPGWGTKWTRS
                     VTTRIDPSQGGTT"
     gene            3910..4188
                     /locus_tag="VNU03_03420"
     CDS             3910..4188
                     /locus_tag="VNU03_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73529.1"
                     /translation="MKLFDPTSKPVERAIKLAPRPASPRGLRLGLVENTKFNASPILE
                     KLAERLAQRHQMTVTVRNRKRSASHEVDGDGIKALRAQADLVISGIGD"
     gene            4210..4437
                     /locus_tag="VNU03_03425"
     CDS             4210..4437
                     /locus_tag="VNU03_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73530.1"
                     /translation="MLDAILMEEQGVPAIAIVTTPFRNTGKAMAESWGKPGYPFLDMP
                     HPIANLTQAQLDERCDRLADQVEEWLGFEAH"
     gene            4454..>5114
                     /locus_tag="VNU03_03430"
     CDS             4454..>5114
                     /locus_tag="VNU03_03430"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HWP73531.1"
                     /translation="MSRIRYDRLPTERVNPETGRLDRLSAAGIARLMNRADRRAVRAV
                     GRAAPAIGRAVDLIVRALSRRGRLIFVGAGTSGRLGVLEAAECPPTFDTPPSLVQAVI
                     AGGKRTVFRSSEGAEDNGPEGARQIRRRVRPGDMVVGIAASGVTPFVRAALKEARRRG
                     AATALVTCNPDVPASAARVVIALAVGPEVLTGSTRLKAGTATKLALNTLTTASFARLG
                     KV"
BASE COUNT          741 a         1851 c         1887 g          635 t
ORIGIN      
        1 ccaccaccat gacttcgacg cagaaggtcg cgatcgtcac cggagcgagc agcggcatcg
       61 gcaaggccag cgccctggcc ctcatgaagg agggctacgc ggtcgtcttc gcggggcgcc
      121 gcaaggacct gctggagtcg gccgccgccg agggcaaggg ctcgccgggc aagtcgctcg
      181 tggtgcagac cgacgtgacc agccacgagt cgatcaaggc gctcttcgcc aagaccaagg
      241 agacgttcgg gcgcctcgac gtgctcttca acaacgcggg cggcggcgcg ccgccggtgc
      301 tgttcgagga cctcacctac gaccagtggc agaacgtggt caacgccaac ctgaccggcg
      361 tcttcctgtg cacccaggag gccttcaaga tcatgaaggc ccagaccccg atgggcgggc
      421 gcatcatcaa caacggctcc atctcggccc acgcgccgcg cccgaactcg gcgccctaca
      481 ccgcgaccaa gcacggggtc accggcctca ccaaggtgag ctcgctcgac ggccgcaagt
      541 acgacatcgc ggtgggccag atcgacatcg gcaacgcggg cactcccatg accgagcgca
      601 tggccaaggg tgtgccgcag gccaacggca ccaccgtggc ggagccgacc atgaacgtga
      661 aggcggtggc cgacgcggtg gtgtacatgt cgagcctgcc gctcgacgcc aacgtgctgt
      721 tcctcaccgt gatggcgacg aagatgccct tcgtcgggcg cggctgatcg tccgctgagc
      781 tcagagcagg gcaacgccgc ctgcccgcag ctccgcttcg ccgaagccga gctcgtccac
      841 cagcaccgcg cggttgtgct cgcccaggcg cgggcccgcg tgccggatcc cgcccggggt
      901 cgcggacagg cgcccgatcg cgttctgcat gcgcagcggc cccaggtccg cgtcgggcac
      961 ggtggcgatg gagccgcgcg cgcgatagtg cgggtcggcc agcacctcgt cggccccgcg
     1021 caccggcgcg gccaccgcct gggcggcgtc gaggcgcgcg agcagatcgg cctgatcgag
     1081 gcggccgatg gccgcctgca gcgcggtgtc gagggcggcc acgttggcca ggcgcgcctg
     1141 gttgtcgcgg aagcgcggat cggcgggcag ctccggaacg cccaggctgc ggcagagccg
     1201 ctcgaacacg gacggagagg cgccggagag cgcgatccac ttgccgtcgc gggtgcggta
     1261 ggtgttacgc ggggcgatca gcggggtgcg gctgccggtg cgcccctgga ccacgccgag
     1321 ctggtcgaac tccacgagct gcgggcccag cagggtgagc agcggctcgt agatcgccag
     1381 gtccaccacc tggccgcgcc cgccccgtag cgcgcgcccc tgcagcgcgg ccagggtgag
     1441 gaacgcaccc gcgaggccgg ccgccgcgtc ggcgagcgcg aagccgggca ggagcggcgg
     1501 gccgtcggcc tcgccgttga tggccgcgaa gccgctggcc gcctccgccg aggtgccgaa
     1561 gccgggccgg tcgcgctcgg gcccggtctg gccgaagccg gtgacgcggg tgaggacgag
     1621 gcccgggttc gcggcggcga gcgtctcgta ccccaggccc catcgctcga gggtgccggg
     1681 ccggaagttc tcgatcaccg cgtcggcgcg cgcggcgagc cggcgcacga tctcgacccc
     1741 gagctcggtg tgcagatcgg cggtgaccga gcgcttgttg cggttcagca tcttgaagta
     1801 gaggccgacg ccgtccttca tcgcgcccca ggcccgcagg ccgtcgccct gcgggtgctc
     1861 gatcttgatc acgtccgcgc cgtagtcgcc caggtacatc gcggcggtgg ggcccgcgat
     1921 cagggaggag cagtcgagca cccgcagccc cgccagcggc ccggcggccc ctgccgtcga
     1981 ttgttccgga tccggaaccg attgcgcatt cacgaacagt catgctacgg tcttccgcga
     2041 tggtgtcaag ggccacggag ccacgcaggg cggagccgga cggcggtcac cggaccgccg
     2101 cgcgggtgct cgacctcctg gaggtgctca gtagccgcgc gggcggcgcg agcctcaccg
     2161 agctcggggt ggcgctcgga gcgcccaaga ccagcctact gccgctgctg cgcaccctgg
     2221 tgcgccgcgg ctacgcggcc cgcggcgact ccgggcgcta cggggtcggc ccgcgctggc
     2281 tcgaggccgg cgagcgaaag gtcgccgccg cgccggacct ggtggcggcc gcccacccgg
     2341 tgctgcaggc gctcacccgc gagaccggcg agaccacctt cgtggcggtg ctgccgcccg
     2401 gccactcgga cctggtctac gtggacaagg tggagagccc gcagcgcatc cgctacagcg
     2461 cggagctggg cgagcgccgc ccgctctact gcaccgccac cggtttcggc ctcttcgcct
     2521 tcctcgagcc cgcgccgcga gagcacctgg tgcgcacgct ccatctcacg cgtcacaccc
     2581 ggcgcaccac cacgagccgc gagctgctgc gccggcggct cgacgccatc cacgcggcgg
     2641 gggtggcggt gacggtggac gagttcgtca tcggcgcggg aggcgcggcc gcgccggtct
     2701 acgggcgcga cgggcgcctg gtcgccgcct gcgcggtgac cggccccacc gcgcggatcc
     2761 gggccggacg gcggcggctc gccgaggccg cgaagcgggc cgcggcggag atctcggcgc
     2821 ggctgggcta ccatgtaccg accggcggcg aggtcaaggc gagggcgcga tgagcgagat
     2881 cgacgaggcg acggacccgg cggcgctgat cgaggcgtac tacgaggcgg gctggacgga
     2941 cggcctgccg gtggtgccgc cgtcggacgc ctccatcgcg gcgatgctcg tcgcggcggg
     3001 gctcgagggc cacgaggagc tgggcgccat caccggccgc aacgcggtgg tcagcgccga
     3061 gaaggccgcg atcaacgcgg tgatggccgg ctgccgcccc gagtacatgc cggtggtggt
     3121 cgcagccgtc cggagcctct gtgcgcccgc cttcgcctac cacggcccgg ccagcagcac
     3181 cggcggctcc gcgatggcgc tcgtggtcaa cggcccgatc gcgccgcggc tcggcatcaa
     3241 cagcggcaac aacgcgttcg gccaggggca ccggcccaac gccaccatcg gccgcgcggt
     3301 gcgcctcatc atgatgaacg tgatgaacac gcgcccgggc ctgctcgacc gcgccaccct
     3361 cggcaacccc ggcaagtaca gcttctgctt cgcggagaac gaggagaatc atccgtggga
     3421 gccgctgcac gtggcgcgcg gcctgcggcg cgaggacagc gcggtcaccc tgatcgcctc
     3481 caacagcctc taccaggtgt acaaccacct cgcggccgat ccggagccgc tgctcctctg
     3541 tttcgccgac gcgctcggca acgtgggcac gccgaacctg ctcggcttca acgagacgct
     3601 catcgtcttc gcgggcgagc acgccgaggt gctgcagaaa gcaggctgga gccgcctcga
     3661 tgtgcagcgc ttcctgatag agcgcacccg gcggtcggtg gccgactaca agcgcggggc
     3721 gcggctgccg ggggcggtca ccgagggcga cgagaccacc tggcgctacc gcttcgagcg
     3781 gcccgaggac atcctgatcg cgttcgcggg cggccgcgcc gggagctggt ccgcctgcct
     3841 gccggggtgg gggacgaagt ggacccgtag cgtcaccacc cgtatcgatc catcccaagg
     3901 aggcaccaca tgaagctctt cgatccgacc tcgaagccgg tggagcgcgc gatcaagctc
     3961 gcgccacggc ccgcgagccc ccggggcctg cggctcgggc tggtggagaa caccaagttc
     4021 aacgcgagcc ccatcctcga gaagctggcc gagcggctgg cccagcgcca ccagatgacg
     4081 gtcacggtgc ggaaccggaa gcggtcggcg agccacgagg tggacgggga cgggatcaag
     4141 gcgctgcgcg cgcaggccga tctcgtcatc tccggcatcg gcgactgagg gtcgtgcagc
     4201 tcgagcagtg tgctcgacgc gatcctgatg gaggagcaag gggtgccggc gatcgccatc
     4261 gtcaccaccc cgttccgcaa taccgggaag gccatggccg agagctgggg caagccgggc
     4321 tacccgtttc tggacatgcc gcacccgatc gccaacctca cgcaggcgca gctcgacgag
     4381 cggtgcgacc ggctggcgga tcaggtcgag gaatggctcg ggttcgaggc gcactagcac
     4441 ttaagatgtc ggcgtgagcc gcatccgcta cgaccggctg cccaccgagc gcgtcaaccc
     4501 ggagaccggc cggctcgacc gcctgtccgc cgccggcatc gcgcggctga tgaaccgcgc
     4561 ggatcgccgc gcggtgcgcg cggtggggcg cgccgcgccc gccatcgggc gcgcggtgga
     4621 tctcatcgtg cgcgcgctct cgcggcgcgg gcggctcatc ttcgtgggcg cgggcaccag
     4681 cgggcggctg ggcgtgctcg aggccgccga gtgcccgccc accttcgaca cgccgccgtc
     4741 gctcgtgcag gcggtgatcg cgggcggcaa gcgcaccgtg ttccgctcct ccgagggcgc
     4801 ggaggacaac gggcccgagg gcgcgcggca gatccggcgc cgcgtgcggc cgggcgacat
     4861 ggtggtgggc atcgcggcca gcggggtgac gcccttcgtg cgcgcggcct tgaaggaagc
     4921 ccggcggcgc ggggcggcga ccgcgctcgt cacctgcaac cccgacgtgc cggccagcgc
     4981 cgcgcgggtg gtcatcgcgc tggcggtcgg ccccgaggtg ctcaccggct cgacgcgcct
     5041 gaaggcgggc accgccacca agctcgcgct caacaccctg accaccgcgt ccttcgcgcg
     5101 gctcggcaag gtct
//
LOCUS       DATMCV010000140         3299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_7542, whole genome shotgun sequence.
ACCESSION   DATMCV010000140 DATMCV010000000
VERSION     DATMCV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7542"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VNU03_03435"
     CDS             <1..871
                     /locus_tag="VNU03_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWP73532.1"
                     /translation="VYNPGSGRQRDRFHSITRMVDALRQQGITATPRTTAAPADATRL
                     AAEALAAGVDLVIVHGGDGTVNEAIQAMVGTPTPLAVWPSGTSNVLARELGLPGSLDL
                     IARRIATGAIRRVSVGRAGERYFLLMAGVGLDADMLRSVHPGLKRLTGEGAYWMAGLR
                     QLTDWNPVPFLVETEGGRHRATFAVVANAASYGGGLRFAPEASMDDDQLDLCLFDSTE
                     RHRFARYLAAAFSGSHIGLPGVTYVKTRRAVAHGPDNRLVQVDGELLGRLPVSFDCIP
                     DALSLVVPLKSPP"
     gene            892..>3299
                     /locus_tag="VNU03_03440"
     CDS             892..>3299
                     /locus_tag="VNU03_03440"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HWP73533.1"
                     /translation="MADAARGAERILEGLNPPQSAAVTHETGPLLIIAGAGTGKTTVI
                     TRRIAYLIATRRARPSEILALTFTDKAAAEMEERVDTLVPYGYADVQISTFHAFGDRL
                     IKENALELGLTPDFRVLTRAEQVIFLRDHLFEFPLKHYRPLGDPTRHLQAILGQFSRL
                     KDEDVGPDEYLTLAEGLLARATDDEARLHAEQHLELARTYAQYQTLMARLGQIDFGDQ
                     IVEALRLFRTRPHVLRRHQERYRHILVDEFQDTNYAQFELVKLLAARQRNVAVVGDDD
                     QAIFRFRGASMSNILAFDRAYPDARQVVLQENRRSPQAVLDAAYRLIQHNNPDRLEVA
                     QKIDKRLISTIGKGEAPEHLPFDTVSTESDQVAEMIQEEHERGRPYRDFAVLVRVNHD
                     ADHYLRALNMRGIPWTFSGNAGLYGRPEVRLLIAFLRSVAHPDDSVSLHYLASSDVYQ
                     VPIVDLTKCATYADRKHRWLFDVLRAVPEEVAITEEGAGAVARLVADLQRYMELAREH
                     PTGEVLYQFLVDSGLMTRYAKAPAELEQEVQNVSKFFTRVKDAARVLKYDNVREFVNH
                     LDALIDAGDDPAVAEADTETPAVHVLTVHKSKGLEWPIMFMVDCMQNRFPSTRRSDPV
                     EIPAELIKDTLPTGDFHEQEERRLFYVGMTRAKERLYLTSAEDLGGKRKWKVSQFILE
                     ALDLPKDAARPFRAQAIEQLRRLAPPPEGIGLALAPIADDEPLAISHRQVDDYETCPL
                     KYQYIHILRIPLRQHHSVVYGSALHNAVEFYLRRRAAGNYTSLEDFLRAFDNAWRNEG
                     FLTR"
BASE COUNT          514 a         1235 c         1073 g          477 t
ORIGIN      
        1 cgtttacaac cccggctccg gccggcagcg cgatcggttt cactccatca cccggatggt
       61 cgatgccctg cgccagcagg gcatcacggc cacgccgcgg accaccgccg cacccgccga
      121 tgcgacccgc ctcgccgcgg aggcgctggc cgccggcgtc gacctcgtga tcgtccacgg
      181 cggtgacggc accgtgaacg aggccatcca ggccatggtc ggcacgccca ccccgctggc
      241 ggtgtggccc tcgggcacgt ccaacgtgct ggcccgcgag ctggggttac ccggcagcct
      301 cgacctcatc gcgcgccgga tcgcgaccgg cgcgatccgg cgcgtgtcgg tgggacgagc
      361 cggcgaacgg tatttcctgc tgatggcggg ggtgggcctc gatgccgaca tgctgcgctc
      421 ggtccacccg ggcctcaagc gcctgaccgg cgagggcgcc tactggatgg ccgggctgcg
      481 ccagctcacc gactggaacc cggtgccctt cctggtggag acggagggcg gccggcaccg
      541 cgccaccttc gcggtggtgg ccaacgcggc gtcctacggc ggcgggctcc gcttcgcgcc
      601 cgaggcgagc atggacgacg atcagctcga cctgtgtctc ttcgactcga ccgagcgcca
      661 tcggttcgcc cgctacctgg ccgccgcgtt cagcggctcg cacatcggcc tgcccggcgt
      721 gacctacgtg aagacgcggc gggcggtcgc ccacgggccg gacaaccgtc tggtgcaggt
      781 cgacggcgag ctactcggcc ggctgccggt cagcttcgac tgcatcccgg atgccctctc
      841 tctggtggtt cccctgaagt cgcctcccta ggggtggtat agtttgccct gatggccgac
      901 gcggcgcggg gcgccgagcg cattctcgag gggctgaacc cgccccagtc tgccgcggtc
      961 actcacgaga ccggcccgct gctcatcatc gcgggcgccg gcaccggcaa gaccaccgtc
     1021 atcacccgcc gcatcgccta tctcatcgcc acgcgccgag cgcgcccctc cgagatcctc
     1081 gcgctcacgt tcacggacaa ggcggcggcc gagatggaag agcgcgtcga caccctcgtg
     1141 ccctacggct acgccgacgt gcagatctcc accttccacg ccttcggcga ccgcctgatc
     1201 aaggagaacg cgctcgagct cgggctcacc ccggacttcc gggtgctcac gcgggccgag
     1261 caggtcatct tcctgcgcga tcacctcttc gagttcccgc tcaagcacta ccggccgctc
     1321 ggcgatccca cccggcatct gcaggcgatc ctcggccagt tcagccggct caaggacgag
     1381 gacgtgggcc ccgacgagta cctgactctc gccgaggggc tcctcgcccg ggccaccgac
     1441 gatgaggccc ggcttcacgc cgagcagcac ctggagctgg cgcgcaccta cgcccagtac
     1501 cagactctga tggcccgcct gggccagatc gacttcggcg accagatcgt ggaggccctg
     1561 cggctcttcc gcacgcgccc gcacgtgctg cgccgccacc aggagcgcta ccggcacatc
     1621 ctggtcgacg agttccagga caccaactac gcgcagttcg agctggtcaa gctcctcgcg
     1681 gcccgccagc gcaacgtggc ggtggtcggc gacgacgacc aggccatctt ccggttccgc
     1741 ggcgcgtcga tgagcaacat cctcgccttc gaccgcgcct acccggacgc gcgccaggtg
     1801 gtgctgcagg agaatcgacg ctccccgcag gcggtgctcg acgcggcgta ccggctgatc
     1861 cagcacaaca acccggaccg gctcgaggtg gcccagaaga tcgacaagcg cctgatctcc
     1921 accatcggga aaggggaggc gcccgagcat ctgcccttcg acaccgtctc caccgagtcg
     1981 gaccaggtgg ccgagatgat ccaggaggag cacgagcgcg gccggccgta ccgcgacttc
     2041 gcggtgctgg tgcgggtcaa tcacgacgcc gaccactacc tgcgcgcgct caacatgcgc
     2101 ggcatcccgt ggaccttctc cggcaacgcg ggtctctacg gccgccccga ggtgcggctg
     2161 ctcatcgcct tcctgcgctc ggtggcccac ccggacgact cggtgagcct gcactacctg
     2221 gcctcctccg acgtctacca ggtgccgatc gtcgatctca ccaagtgcgc gacctacgcc
     2281 gaccgcaagc accgctggct cttcgacgtg ctgcgcgcgg tgcccgagga ggtggcgatc
     2341 accgaggagg gcgcgggcgc ggtggcccgg ctcgtggcgg acctgcagcg ctacatggag
     2401 ctggcccgcg agcacccgac cggcgaggtc ctctatcagt tcctggtcga ctcgggcctc
     2461 atgacccgct atgcgaaggc ccccgccgag ctcgagcagg aggtgcagaa cgtcagcaag
     2521 ttcttcacgc gggtgaagga cgcggcgcgg gtgctcaagt acgacaacgt gcgcgagttc
     2581 gtgaaccacc tggacgcgct gatcgacgcg ggcgacgatc ccgcggtggc cgaggccgac
     2641 accgagaccc cggcggtgca cgtgctcacc gtgcacaagt cgaaggggct cgagtggccg
     2701 atcatgttca tggtggactg catgcagaac cggtttccgt ccacccgccg gtccgacccg
     2761 gtcgagatcc ccgccgagct gatcaaggac accctgccca ccggcgactt ccacgagcag
     2821 gaggagcgcc gcctgttcta cgtgggcatg acccgcgcca aggagcgcct ctacctcacg
     2881 agcgcggagg acctcggcgg caagcgcaag tggaaggtga gccagttcat cctggaagcg
     2941 ctcgacctgc ccaaggacgc ggcgcggccc ttccgggccc aggccatcga gcagctacgg
     3001 cgcctggccc cgccccccga gggcatcggg ctcgccctcg ccccgatcgc ggacgacgag
     3061 ccgctcgcga tcagccaccg gcaggtggac gactacgaga cctgtccgct caagtaccaa
     3121 tacatccaca tcctgcgcat cccgctgcgg cagcaccact cggtggtcta cggcagcgcg
     3181 ctccacaacg cggtcgagtt ctacctgcgc cgtcgcgcgg ccggcaacta cacctcgctc
     3241 gaggacttcc tgcgcgcgtt cgacaacgcg tggcggaacg agggctttct cacccgcga
//
LOCUS       DATMCV010000141         3558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_873920, whole genome shotgun sequence.
ACCESSION   DATMCV010000141 DATMCV010000000
VERSION     DATMCV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873920"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VNU03_03445"
     CDS             complement(<1..804)
                     /locus_tag="VNU03_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWP73534.1"
                     /translation="MRIVSIEPILVDVPLAEPVHGVHGVTTMQRSVLVRVTSDDGAEG
                     WGNVDPTPGYSAMSAAEVHDTVRRIGPALAGADPFNVHCALAVMDREAPGRYETIAAV
                     EMAMVDLKARALDLPVHSLLGGRVKDEVTFNAWIGTVPPDRAAHEAMEWLGRGFRSAK
                     IKVSGTASDGIARVAAVRAAVGDRMALRVDFNESLTRAEAVPFIRLLEPYALTLVEQP
                     IPRGEIAGLAEIRRAIGIPLMADESVTGPASLVEIIRREAADLVKVKVMK"
     gene            complement(816..1211)
                     /locus_tag="VNU03_03450"
     CDS             complement(816..1211)
                     /locus_tag="VNU03_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP73535.1"
                     /translation="MAIQLDHVIVPSRDPIAAARSLAGLLDVPWQESQGPFTPVYVND
                     SLTLDFADRERFESHHYCFRVSDAEFDAIFGRIQAARIPYRSSPLGETDMQINRRLGG
                     KNLYWQDGDKHLWEILTVSYARPDRVSVA"
     gene            1360..2349
                     /locus_tag="VNU03_03455"
     CDS             1360..2349
                     /locus_tag="VNU03_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HWP73536.1"
                     /translation="MITTYQAHELALVFPPMTELEFAAFKEDIRENGQREPITLYEGK
                     ILDGLHRYRACQELGLEPRVVRFEGNPRAAAQLVLGRNFHRRHLTTSQRAMVAAEMCK
                     LRPRGNTGNSPYLTAAQASALMGVGEDLVRDAKHLLRQGDEDTLRAVREGLQSVSAAV
                     QEKEKKKKATFTFLEQFAQRLDRALQGHNDRDAQAIYRATPLLQYLNLRIHEYLHPRT
                     AEPLKTGDGSVRWDPAELKVPHWLDVERGQELATERRARLATVLPKAKEAAMAAPGER
                     CPACGLPLLSAVKGTSYCAVCKGDVLWEWSWIREQVEIAFGSVPLTSSPPALG"
     gene            2354..2725
                     /locus_tag="VNU03_03460"
     CDS             2354..2725
                     /locus_tag="VNU03_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73537.1"
                     /translation="MARILVVDDQPEVAELVQAVLKDEGHAADAVYSGRAALDRLAQT
                     VYDLVVCDVRMPDVDGKAVFRAVAQLGSPRPVVLFMTGYGDSPTDSEFLQTTAAVVLA
                     KPLGIDALLERVRCLLADSLT"
     gene            2821..>3558
                     /locus_tag="VNU03_03465"
     CDS             2821..>3558
                     /locus_tag="VNU03_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73538.1"
                     /translation="MVRSVSIYQSLLGNLALVIVLLSGAIMALTFIGSRATVERLSGV
                     ILRETIGQTEGELRHFFEPVIRDLGVMRGWSEAGLLDDGDAAAMNRLLVPLLRADPQL
                     SAVMVADERGREHIVFHFGAAWSSRQTRRDVWGDRSAWLEWTDARPEPVASQRASDYD
                     PRRRPWYQGAVSARSGAASQVSASEIVYWTEPYTFYTAKAPGMTAAMAFPARNGQLSV
                     VGADLLLTDISTFTTRLRVGEHGAVMVL"
BASE COUNT          536 a         1237 c         1249 g          536 t
ORIGIN      
        1 cttcatgacc ttgaccttga ccaggtcggc cgcctcgcgc cggatgatct cgaccaggga
       61 ggcggggccg gtcacgctct cgtcggccat gagcgggatg ccgatggccc ggcggatctc
      121 ggcgagcccg gcgatttccc cgcgcgggat gggctgctcg accagcgtca gggcgtaggg
      181 ctcgaggagc cggatgaacg gcaccgcctc cgcccgcgtg aggctctcgt tgaaatcgac
      241 ccgcagcgcc atccggtcgc ccaccgccgc ccgcaccgcg gcgacgcggg cgatcccgtc
      301 ggacgcggtg cccgatacct tgatcttggc cgagcggaag ccacgcccga gccattccat
      361 cgcctcgtgc gcggcccggt cgggtggcac cgtgccgatc caggcgttga acgtgacctc
      421 gtccttcacg cgcccgccca ggagcgagtg gacgggcagg tcgagcgcgc gcgccttgag
      481 gtcgaccatg gccatctcga ccgcggcgat cgtctcgtag cgccccggcg cctcgcggtc
      541 catgacggcg agggcgcagt gcacattgaa cggatcggcc ccggccagcg ccgggccgat
      601 ccgccggacg gtgtcgtgga cctccgccgc cgacatggcc gagtagcccg gcgtgggatc
      661 cacgttgccc cagccttcgg cgccgtcgtc gctcgtgacg cgcacgagca cgctgcgctg
      721 catggtggtg acgccatgca cgccgtggac gggctcggcc aggggcacgt ccacgaggat
      781 cggctcgatg ctgacgatgc gcatgcggcc cgccctcacg ccaccgagac gcggtccgga
      841 cgcgcgtagc tgacagtgag gatctcccag aggtgcttgt ccccgtcctg ccagtagagg
      901 ttcttcccgc cgagcctccg gttgatctgc atgtcggtct ctccgagcgg cgagctccgg
      961 tacgggatgc gcgccgcctg gatgcggccg aagatcgcgt cgaactcggc gtcgctcacg
     1021 cggaagcagt agtgatggct ctcgaagcgt tcccgatccg cgaagtcgag agtcagcgag
     1081 tcgttgacgt agaccggcgt gaatggcccc tgcgactcct gccacgggac gtcgagcagg
     1141 ccggcgagtg agcgcgccgc tgcgatcgga tcacgagagg ggacgatgac gtggtcgagc
     1201 tgaatcgcca tgaagcacct ccagcgatgg tcttctacgg gcgcagcatg ctcgccgggc
     1261 gaggcaccgt caagacggcc tcgggcgggc gccagtcata tgttgggtat catgaaagaa
     1321 ttccgcaccc cctatgctgg gaggccagga ggggacgcga tgatcaccac ctatcaagcc
     1381 cacgagttgg cgctggtctt tcccccgatg accgagctgg agttcgccgc gttcaaggag
     1441 gacatccgcg agaacggaca gcgcgagccc atcacgctct acgagggcaa gatcctggac
     1501 gggctgcacc ggtatcgcgc ctgccaggag ctggggctgg agccgcgagt ggtgcgcttc
     1561 gagggaaacc cgcgcgccgc cgcgcagctg gtcctcggcc ggaacttcca ccggcggcac
     1621 ctcacgacgt cccagcgggc gatggtcgcg gccgagatgt gcaagctccg gccccggggt
     1681 aataccggga attcccccta tttgaccgcg gctcaggcct ccgccctgat gggcgtcgga
     1741 gaggacctcg tgagggatgc caagcacctg ttgaggcagg gcgacgagga caccctgcgt
     1801 gcggtccgcg aagggcttca gtccgtctcg gccgcggtcc aggaaaaaga gaaaaagaag
     1861 aaggccacgt tcaccttcct cgagcagttc gcccagcgcc tcgaccgcgc gctccagggc
     1921 cacaacgatc gcgacgccca ggccatctac cgggcgacgc cgctgctgca gtacctcaac
     1981 ctccgcatcc acgagtacct gcaccctcgc acggccgagc ccctgaagac aggcgacggc
     2041 tccgtgcggt gggatcccgc ggagctcaag gtgccgcact ggctcgacgt cgagcgcggc
     2101 caggagctcg ccaccgagcg ccgggcccgg ctcgcgacgg tcctgcccaa ggcgaaggaa
     2161 gcggccatgg ccgccccggg ggagcgctgc ccggcctgcg ggttgcccct gctgtcggcc
     2221 gtgaagggca cctcgtactg cgccgtctgc aagggcgacg tgctgtggga atggtcgtgg
     2281 atccgggagc aggtcgagat cgccttcggc agcgtccccc tcaccagctc gccgccggcg
     2341 ttgggctgac cgcatggccc gcatcctggt ggtggacgac cagcccgagg tcgcggagct
     2401 ggtgcaggcg gtgctcaagg atgaagggca cgcggcggac gccgtctaca gcggccgagc
     2461 ggccctggac cgcctcgccc agaccgtgta cgacctggta gtctgcgatg tgcggatgcc
     2521 ggacgtcgac ggcaaggcgg tcttccgcgc cgtcgcgcag ctggggtcgc cgcggccggt
     2581 cgtgctgttc atgaccgggt acggcgatag cccgaccgac tcggagttcc tgcagacgac
     2641 cgcggcggtg gtcctcgcca agccgctcgg catcgacgcg ctgctcgagc gcgtccgctg
     2701 cctcctcgcc gattcgctga cgtagcctcg cgcgatcccg cttgacgcgc cgcggtttcg
     2761 ggtccagcct cgctggcgtc cgagtcccgg cggcccgccg tgccggccca ggagagcgtc
     2821 atggtccgct cggtgtccat ctaccagagc ctgctgggca acctcgccct cgtgatcgtg
     2881 ctgctgagcg gcgcgatcat ggcactcacc ttcatcggct cgcgggccac cgtcgagcga
     2941 ctctccggcg tgatcttgag agaaaccatc ggccagaccg agggcgagct gcgccatttc
     3001 ttcgagccgg tgatccggga tctcggcgtg atgcgcgggt ggagcgaggc ggggctgctc
     3061 gacgatggag acgcggccgc catgaaccgg ctgctcgtcc cgctgctgcg cgccgatcct
     3121 cagctgtcag cggtgatggt ggcggacgag cgcggccgcg agcacatcgt gttccacttc
     3181 ggcgcagcgt ggagcagccg gcagacccga cgcgacgtct ggggtgaccg ctccgcctgg
     3241 ctcgagtgga cggatgcgcg accggagccc gtcgcctcgc agcgggcgag cgactacgac
     3301 ccccgacggc gtccctggta ccagggggcc gtctcggcgc gctccggcgc cgccagtcag
     3361 gtcagcgctt cggagatcgt ctactggacg gagccgtaca cgttctacac cgccaaggcc
     3421 ccgggcatga cggcggcgat ggcgttcccg gcacgcaacg gccagctgag cgtcgtcggc
     3481 gccgacctgc tgctgaccga catctccacc ttcaccacac gcctgcgggt tggggagcac
     3541 ggggcggtaa tggtcttg
//
LOCUS       DATMCV010000142         2052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_804689, whole genome shotgun sequence.
ACCESSION   DATMCV010000142 DATMCV010000000
VERSION     DATMCV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804689"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VNU03_03470"
     CDS             <1..1336
                     /locus_tag="VNU03_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP73539.1"
                     /translation="HWSDVGGPVPSSMQIGSREFRQEGLRFQSVRLFDRGECNEEVMR
                     MIRGNIRTESGTLKDIQAMIAVCRAGEAYFREILGKYGRDTVLESTRVYLEQSERRTR
                     AELARIPAGTYRAVAHFDNDGITLDQPVRIEMAITLDDGAGFPPGRPTMIVDFTGSSP
                     QVVGPFNCGDAITISACRLLIKCLSTPLTPVDEGCFRPLQVIIPERSVLACQEPAPTA
                     RYYVPINLLIDLGLRALSEAMPDRIPAGSYGDQMPTIAFGTHPETGRLFIQGDLNAGG
                     TGGRPAYDGESAMIIFAGSTARNNPVEVVESRTPSIRILRYGLRPDSGGAGRYRGGLG
                     IERLYEFLAPAFITFSLERKVTPPWGLRGGRDGAVNAVEITAPDGAVRHVRKATQHPI
                     EAGERVRILTGGGGGYGPPAERDPEAVRRDVAEGYVSGEAARRDYGAAATGA"
     gene            1347..>2052
                     /locus_tag="VNU03_03475"
     CDS             1347..>2052
                     /locus_tag="VNU03_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP73540.1"
                     /translation="MAIRAIRCGTLFDATGAAAIRQAMVVVDGGQITAAGPAASTPVP
                     AGAEVLDWSDRFVMPGLVDAHSHISIVPGDGDQIGQLRQDVVPQALRATSNLRRDLAA
                     GTTTLRVMTEERFLDVQVREAIAAGVIPGPRLLCGTRGITASNGHGRAHSSFDGVDAI
                     RRGVRENFQGGADHVKIFATGGVSSPGTTLNASVYSREEIRAAVEEAERAGKYAAAHA
                     HGGPGLRLCVEEGVSTI"
BASE COUNT          288 a          748 c          746 g          270 t
ORIGIN      
        1 ccactggagc gacgtgggcg gcccggtgcc gagctccatg cagatcggct cgcgcgagtt
       61 ccggcaggag gggctgcgct tccagtcggt gcgcctcttc gaccggggtg agtgcaacga
      121 agaggtgatg cggatgatcc gcggcaacat ccgcaccgag agcggcacgc tcaaggatat
      181 tcaagcaatg atcgcggtgt gcagggcggg ggaggcctac ttccgcgaga tcctgggcaa
      241 gtacgggcgt gacaccgtcc tcgagtcgac ccgcgtctac ctcgagcagt cggagcgccg
      301 cacccgcgcc gagctggccc gcatcccggc cggcacgtac cgcgcggtcg cccacttcga
      361 caacgacggc atcacgctcg accagccggt ccgcatcgag atggcgatca ccctcgacga
      421 cggcgccggc tttccgcccg ggcggcctac gatgatcgtc gacttcaccg gctcgagccc
      481 gcaggtggtg ggcccgttca actgcggcga cgccatcacg atcagcgcgt gccgcctcct
      541 gatcaagtgc ctctccaccc cgctcacccc ggtggacgag ggctgcttcc gcccgctcca
      601 ggtgatcatc ccggagcgct cggtgctggc ctgccaggag ccggcgccga ccgcgcgcta
      661 ctacgtgccg ataaatcttc tgatcgatct gggcctgcgg gcgctctcgg aggcgatgcc
      721 cgaccgcatc ccggccggct cctacggtga ccagatgccg acgatcgcct tcggcaccca
      781 tccggagacc ggccgtctct tcatccaggg cgatctcaac gcgggcggca ccggagggcg
      841 gccggcctac gacggcgagt cggccatgat catcttcgcg gggtcgaccg cgcggaacaa
      901 cccggtcgag gtggtggaat cccgcacccc gtccatccgc atcctgcgtt acgggctgcg
      961 ccccgactcc ggcggcgccg ggcgctaccg cggggggctc ggcatcgagc gcctctacga
     1021 gttcctcgcc cccgcgttca tcaccttcag cctcgagcgg aaggtgacgc cgccgtgggg
     1081 gctgcggggc ggccgcgacg gggcggtgaa cgcggtcgag atcaccgcgc ccgacggggc
     1141 ggtgcggcac gtgcgcaagg cgacccagca cccaatcgag gccggggagc gcgtgcgcat
     1201 cctgaccggc gggggcggcg gctacgggcc tccggccgag cgcgatccgg aggcggtgcg
     1261 ccgcgacgtg gccgagggct acgtgagcgg cgaggcggcg cggcgcgact acggcgcggc
     1321 ggcgaccggg gcctaggggg cgcccggtgg cgatccgggc gatccgctgc gggacgctgt
     1381 tcgacgccac cggcgccgcc gcgatccgcc aggcgatggt cgtggtggac ggcgggcaga
     1441 tcacggccgc cggcccggcg gcgtccaccc ctgtccccgc gggcgccgag gtcctcgact
     1501 ggagcgaccg cttcgtcatg ccgggcctcg tggacgccca cagccacatc tcgatcgtgc
     1561 cgggggacgg tgaccagatt ggtcagctcc gacaggatgt tgtcccacag gccctgcggg
     1621 caacctcgaa cctccgtcgc gacctggccg ccggcaccac cacgctgcgg gtgatgaccg
     1681 aggagcgctt cctcgacgtc caggtgcggg aggccatcgc ggcgggggtg atccccggcc
     1741 cgcggctcct ctgcggcacc cgcggcatca ccgcgagcaa cgggcacggg cgcgcccact
     1801 cctccttcga cggggtcgac gcgatccgcc gcggggtgcg cgagaacttc cagggcgggg
     1861 cggaccacgt gaagatcttc gccacgggcg gggtcagctc ccccggcacc acgctcaacg
     1921 cgagcgtcta ctcgcgcgag gagatccggg ccgcggtgga ggaggcggag cgtgccggca
     1981 agtacgcggc ggcccacgcc cacgggggcc cgggcctgcg cctctgcgtc gaggagggcg
     2041 tctccaccat cg
//
LOCUS       DATMCV010000143         4261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_671285, whole genome shotgun sequence.
ACCESSION   DATMCV010000143 DATMCV010000000
VERSION     DATMCV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671285"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..2360
                     /locus_tag="VNU03_03480"
     CDS             351..2360
                     /locus_tag="VNU03_03480"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HWP73541.1"
                     /translation="MTARRATGIGFAEIVSSEPTAWEIQTTGPGPQGSLPLTAEMLLE
                     RPSGDIFGMTQNAGMGWDPRELGRPQFLMLSTQGGIRAADGRPIALGYHTGHWEIGLL
                     MQAAAEELVALGGLPFAGFCSDPCDGRTQGTPGMMDSLAYRNDAAIVLRRLARSLPTR
                     KGVLGVATCDKGLPAMMMALAALRDLACVLVPGGVTLPPQEGEDAGTVQAIGARFAHG
                     ELTLERAAELGCRACGSPGGGCQFLGTAATAQVVGEALGLSLPHSALAPSGQPIWLDM
                     ARRSARALVSLEARGLATRDVVTDAAVRNAMMVHAAFGGSTNLLLHLTAVAHAAGLRR
                     PVVDDWIDVNRRVPRLVDVLPNGPRNHPTVRVFLAGGVPEVMLHLRRGGLLDERCLTV
                     SGETLGRVLDWWEGSERRRAMRERLRAQDGVDPDDVIMDPEQARTRGLTSTVTFPRGN
                     LAPEGAVIKSTAIDPSVVDADGVYRKVGPARVFTTERAAIAAIKSTGPERVKPGDVMV
                     LICRGPLGAGMEEIYQITSALKHISWGKQVAVITDARFSGVSTGACIGHVGPEALAGG
                     PIGRLRDGDLVQIVIDRLKLEGAVDLVGADGVTFGAAEGARVLATRAPRPDLAPDPGL
                     PDDTRLWAALQHAGGGAWGGCVYDVDAIVHALSTRPPLPSGERVG"
     gene            complement(2377..2667)
                     /locus_tag="VNU03_03485"
     CDS             complement(2377..2667)
                     /locus_tag="VNU03_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HWP73542.1"
                     /translation="MWASTESPQVDDAEATDVAVAPPWMTVLHNCDCHTFEQVVQQLM
                     KAIACSEERGWELAWEVHNSGRAVVKVGAETECVRVGNILAAIGLVVTVTQS"
     gene            complement(2676..3872)
                     /locus_tag="VNU03_03490"
     CDS             complement(2676..3872)
                     /locus_tag="VNU03_03490"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP73543.1"
                     /translation="MSRPPLDGLRILAVSQFGAGPFGTQMLADLGADVIKVEDPGVGG
                     DSARYVPPFQGEADSPYFQSFNRGKRSVSLNLRHPDGQAVLRDLVRVSDAVFNNARGD
                     LPDKLGLTYEQLKDTNPRVVCCSLTGYGRTGPRAGEPAFDYLVQGYAGYMSVTGEPDG
                     PPGKCGVSVIDFAGGYAAMVGLMVGLFDAQRTGVGRDVDISLLDTAVSMLSYFAIWTL
                     NRDWIAERTHESAHQTLVPAQNFPTRDGWIVIFCNKDKFWRDLVETLGVPELAEDERF
                     RTFADRFTNRDALVSLLHARFTTRTTADWLDRLRGRVPCAPVNDVRQALADPQVLARD
                     MIVEVEHPEFGTLREVRSPVRTEGEIRRPQRAPRLGEHTDPILRDVLGYSDSTIARLR
                     SAGVIG"
     gene            complement(3874..>4261)
                     /locus_tag="VNU03_03495"
     CDS             complement(3874..>4261)
                     /locus_tag="VNU03_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP73544.1"
                     /translation="ALLTMNQHPLHSDAHYAQTYTQHKQRVVMGPLVYSVVIGMTVAD
                     ISGRAIANLEVNALKHEKPTFIGDTLYARTRVLELRESSQGDRGIVTVATEGFNQRNE
                     VVCSYTRKVLVPKKSHATLGEGRLPY"
BASE COUNT          589 a         1460 c         1607 g          605 t
ORIGIN      
        1 gctgccggtg cccgcgtaga ccgcggcgca ggccgcagcg gtggtgacga gatgtcctcc
       61 ggggctcatg gcgcttcggc taccgcgcgt aggggcggcg gaaacgacgg accggaaccg
      121 gaaccggccg gcggtcgagc cacccgagcg ggattgtgtc gatcaagtcg aggatgcgaa
      181 gaccgagtcg cttcaacatg gactccagag ggagcaactc cggtgccagt ggcgccccgg
      241 ctgcaagtag ctgaattttc gagagaccat agggctgggt ggggacttcg cgggccaaaa
      301 caggaagatc ctaggaccaa aatggggaga tcgatgccag gagagtgggc atgacggcca
      361 gaagggcaac cgggatcggc ttcgctgaga tcgtctcctc cgagcccacc gcctgggaga
      421 tccagaccac cggtccgggc ccgcagggca gcctgcccct caccgccgag atgctgctcg
      481 agcggccgag cggcgacatc ttcgggatga cccagaacgc gggcatgggc tgggatccgc
      541 gcgagctggg ccggccccag ttcctgatgc tgtccacgca gggcggcatc cgcgccgccg
      601 acgggcgtcc catcgcgctc ggctaccaca ccgggcactg ggagatcggc ctgctcatgc
      661 aggccgcggc ggaggagctg gtggcgctcg gcggcctgcc cttcgccgga ttctgctcgg
      721 acccgtgcga cgggcgcacc cagggcacgc ccggcatgat ggacagcctg gcctatcgca
      781 acgacgccgc catcgtgctg cggcggctgg cgcgctcgct gcccacccgc aagggcgtgc
      841 tgggagtggc cacctgcgac aagggcctgc cggccatgat gatggcgctg gccgccctgc
      901 gcgacctcgc gtgcgtgctc gtgcccggcg gcgtgaccct gccgccgcag gagggggagg
      961 acgcgggcac cgtgcaggcc atcggggcgc gcttcgccca cggcgagctc acgctcgagc
     1021 gggccgccga gctgggctgt cgtgcctgcg gctccccggg tggtggctgc cagtttctcg
     1081 gcaccgcggc caccgcgcag gtggtggggg aggccctcgg gctctcgctg ccgcacagcg
     1141 cgctcgcgcc ctcgggccag ccgatctggc tcgacatggc gcggcgctcg gcccgcgcgc
     1201 tcgtctcgct cgaggcgcgt gggctcgcca cccgcgacgt cgtcaccgac gcggcggttc
     1261 gcaacgccat gatggtgcat gcggccttcg ggggctcgac caacctcctg ctgcacctga
     1321 ccgcggtggc gcacgcggcg ggcctgcggc gcccggtggt ggacgactgg atcgacgtca
     1381 accgtcgcgt gcctcgcctg gtcgacgtgc tgccgaacgg gccccgcaat catccgacgg
     1441 tgcgggtgtt cctggccggc ggggtgcccg aggtgatgct ccacctgcgg cgcggcggcc
     1501 tcctcgacga gcggtgcctc accgtgtccg gcgagaccct ggggcgcgtg ctcgactggt
     1561 gggagggctc cgagcggcgc cgcgcgatgc gcgagcggct gcgcgcgcag gacggggtcg
     1621 atccggacga cgtcatcatg gacccggagc aggcgcgcac ccgcggcctc acgagcacgg
     1681 tcacctttcc ccggggcaac ctggcgccgg agggcgcggt gatcaagtcc accgcgatcg
     1741 acccgtcggt ggtggacgcg gacggggtct accgcaaggt ggggcccgcc cgcgtgttca
     1801 ccaccgagcg cgcggcgatc gccgcgatca agagcaccgg gcccgagcgg gtcaagccgg
     1861 gcgacgtgat ggtgctgatc tgtcgcgggc cgctgggggc gggcatggag gagatctacc
     1921 agatcacctc cgcgctcaag cacatctcgt ggggcaagca ggtggcggtc atcaccgacg
     1981 cgcgcttctc cggcgtctcc accggggcct gcatcggcca cgtggggccg gaggcgctcg
     2041 cgggtggccc catcggccgc ctgcgcgacg gcgacctcgt gcagatcgtg atcgaccggc
     2101 tgaagctcga gggcgcggtg gatctggtgg gagcggacgg ggtcaccttc ggggcggcgg
     2161 aaggcgcgcg ggtgctcgcg acccgggcgc cgcggccgga cctcgcgccc gatccgggcc
     2221 tgcccgacga cacgcggctc tgggccgcgc tccagcacgc gggcggcggg gcctggggcg
     2281 gctgcgtcta cgacgtcgac gccatcgtcc acgccctctc cacgcgcccc cctctcccct
     2341 caggggagag ggtagggtga ggggtccgac gccgcgctac gactgggtga cggtgaccac
     2401 gaggccgatg gcggcgagga tgttcccgac gcgcacgcac tcggtctcgg cgccgacctt
     2461 gaccaccgcc cgcccggagt tgtgcacctc ccacgcgagc tcccagccgc gctcctccga
     2521 gcaggcgatc gccttcatga gctgctgcac gacctgctcg aaggtatggc agtcgcagtt
     2581 gtggagcacg gtcatccacg ggggcgccac cgccacatcg gtggcctcgg catcgtcgac
     2641 ctgggggctc tcggtcgaag cccacatcac cgcggctacc cgatgacgcc ggccgagcgg
     2701 aggcgcgcga tcgtgctgtc gctgtagccc aggacgtcgc gcaggatcgg gtcggtgtgc
     2761 tcgccgaggc gcggggcccg ctgcggccgg cggatctcgc cctcggtgcg gacggggctc
     2821 cgcacctcgc gcagggtccc gaactcgggg tgctccacct ccacgatcat gtcgcgggcc
     2881 agcacctggg ggtcggccag ggcctggcgc acatcgttga ccggcgcgca cggcacgcgg
     2941 ccgcgcagcc ggtcgagcca gtcggcggtg gtgcgggtgg tgaagcgcgc gtgcagcagc
     3001 gagaccaagg cgtcgcggtt ggtgaagcga tcggcgaagg tgcggaagcg ctcgtcctcg
     3061 gcgagctcgg ggacgccgag ggtctcgacc aggtctcgcc agaacttgtc cttgttgcag
     3121 aagatcacga tccagccgtc gcgggtgggg aagttctgcg ccgggacgag ggtctggtgg
     3181 gccgactcgt gggtgcgctc ggcgatccag tcccggttga gggtccagat cgcgaagtag
     3241 gagagcatgc tcaccgcggt gtccagcagc gagatgtcga cgtcgcgccc gacgccggtg
     3301 cgctgggcgt cgaagagccc gaccatcagg cccaccatcg cggcgtagcc gcccgcgaag
     3361 tcgatcaccg agaccccgca cttgcccggc gggccgtcgg gctcgccggt caccgacatg
     3421 tagcccgcgt agccctgcac caggtagtcg aaggcgggct cgcccgcgcg cggtccggtg
     3481 cggccgtagc cggtgagcga gcagcagacc acgcgcgggt tggtgtcctt gagctgctcg
     3541 taggtgaggc cgagcttgtc gggcaggtcg ccgcgcgcgt tgttgaagac cgcgtccgag
     3601 acgcgcacca ggtcgcgcag gaccgcctga ccgtccggat gccgcaggtt gagcgagacc
     3661 gagcgcttcc cgcggttgaa ggactggaag tagggcgagt cggcctcccc ctggaacggc
     3721 ggcacgtagc gcgcgctgtc gccgcccacc cccggatcct cgaccttgat gacgtcggcg
     3781 cccaggtcgg cgagcatctg ggtgccgaag gggccggcgc cgaactggga gaccgcgagg
     3841 atgcggagac cgtcgagcgg cgggcggctc atctcaatat gggagccggc cctcgcccag
     3901 ggtggcgtgg ctcttcttcg gcacgaggac cttccgggtg taggagcaca ccacctcgtt
     3961 ccgctggttg aagccctcgg tcgccaccgt gacgatcccg cggtcgccct ggctcgactc
     4021 ccggagctcc agcacgcggg tgcgcgcata gagggtgtcg ccgatgaagg tgggcttctc
     4081 gtgcttgagc gcgttcacct cgaggttggc gatggcgcgc cccgagatgt cggccaccgt
     4141 catgccgatc accacgctgt agacgagcgg gcccatcacc acccgctgct tgtgctgggt
     4201 ataggtctgc gcgtagtggg cgtcggagtg gagcgggtgc tggttcatgg tcaggagcgc
     4261 g
//
LOCUS       DATMCV010000144         2222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_7722, whole genome shotgun sequence.
ACCESSION   DATMCV010000144 DATMCV010000000
VERSION     DATMCV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7722"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2222)
                     /locus_tag="VNU03_03500"
     CDS             complement(<1..>2222)
                     /locus_tag="VNU03_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HWP73545.1"
                     /translation="LAGRYERHLAETPGDLGDVCFTAATGRAQLPHRLAAVVHSAAEA
                     RETLARLAAGEATARAHRGRARSPRPRVAFLFTGQGAQYVGMGRELYRRHETFRRILD
                     RCDSLLRPHLSEPLLSVMFGEAGTPALLDETQYTQPALFALEYALSETWREWGVTPSF
                     VLGHSVGEYVAACVAGLMTLEDALGLIAIRGRMMQALPAGGAMAAVFAPEAAVAEALR
                     EHSDRLALAAVNAPDSVVISGAAAAVAAVVDGLAARGIRTQPLTVSHAFHSPLMDPML
                     DGFAAAAGRVRFGRPRVGIVSNVTGRLASAQEMSSAEYWSRHVRAPVRFAAGVTALRE
                     QGVDIFLEAGPAPTLLGLGRRTLGDEGTVWVPSLRKGRDDWDEMLEGLGRLHVEGVEV
                     DWAGLDRPYARRRTVLPTYPFQRERYWVETKAASPAAEPVDGHPLVGRRVTSPRLHES
                     VFQYVVRADQPAFVRDHQVHGTIVFPGTGYLESMLAAARTALGDAPLDLLDVAIRAPM
                     IVDPEAGRMVQVLVGALAEGAAPVELWSRPVDDDRPEAWQCHATARVGRREGDGCGHP
                     GDHQAAGRERCREAVAVSEFYQRLAGIGLDYGETFRGVREVWRGEDESLGLVEVEGAQ
                     LADAAAYQAHPALLDACLHVFGAAVASDESAADDVYVPVGLGRFQLLRPIGSRLWTRA
                     TVTRRQPAEGILEGDLHLFDDAGELVACIGGLSLRRMPPEALGRAATAGWLYEVAWRP
                     "
BASE COUNT          308 a          865 c          796 g          253 t
ORIGIN      
        1 gggggcgcca cgccacctcg tacagccagc ccgcggtggc cgcgcggccc agcgcctcgg
       61 gcggcatccg ccgcagcgag aggccgccga tgcacgccac cagctcgccc gcgtcgtcga
      121 acaggtggag atcgccctcg aggatgccct ccgccggctg ccgccgggtc accgtcgcgc
      181 gggtccagag gcggctcccg atcggtcgca ggagctgaaa gcgcccgaga ccgaccggca
      241 cgtacacatc gtcggccgcg gactcgtccg aggccaccgc ggcgccgaac acgtgcaggc
      301 acgcgtcgag cagcgcggga tgcgcctggt acgcggccgc gtccgcgagc tgtgccccct
      361 ccacctccac gaggcccagg ctctcgtcct cgccgcgcca gacctcccgg accccgcgga
      421 acgtctcgcc gtagtcgagc ccgatcccgg ccaggcgctg atagaactcg gagacggcga
      481 cggcctcgcg gcagcgttcg cgcccggcgg cctggtggtc gcccgggtgg ccgcagccgt
      541 cgccttcgcg gcgccccacc cgcgcggtcg cgtggcactg ccaggcctcc ggccgatcgt
      601 cgtcgaccgg acggctccac agctccaccg gcgccgcgcc ctccgcgaga gcgcccacca
      661 gcacctgcac catccggccc gcctcggggt cgacgatcat cggcgcgcgg atcgccacat
      721 ccaggaggtc gagcggcgca tcgcccagcg cggtgcgcgc ggccgccagc atgctttcga
      781 ggtagccggt gcccgggaac acgatcgtcc cgtggacctg gtgatcgcgc acgaacgcgg
      841 gctgatcggc gcggacgacg tactggaaga ccgactcgtg gagccgtggc gaggtcacgc
      901 gacgcccgac gaggggatgt ccgtcgaccg gctccgccgc cgggctcgcg gccttcgtct
      961 cgacccagta ccgctcgcgc tggaagggat aggtcgggag cacggtccgg cgccgcgcgt
     1021 aggggcggtc gagaccggcc cagtccacct cgacgccttc cacgtggagt cggcccaggc
     1081 cctccagcat ctcgtcccag tcgtcccgcc ccttgcgcag ggacggcacc cacaccgtgc
     1141 cctcgtcgcc cagcgtgcgg cggccgaggc ccagcagggt gggcgccgga cccgcctcga
     1201 gaaagatgtc gacgccctgc tcgcggaggg cggtcacgcc ggccgcgaac cggacggggg
     1261 cgcgcacgtg acggctccag tactcggcgc tcgacatctc ctgcgcgctg gcgaggcggc
     1321 cggtcacgtt ggagacgatg ccgacgcgcg gccggccaaa cctgacgcga ccggcggcgg
     1381 cggcgaagcc gtccagcatc gggtccatca ggggcgaatg gaaggcgtgg gacacggtca
     1441 agggctgggt ccggatcccg cgagcggcga gcccatccac caccgcggcg accgcggcgg
     1501 ccgcgcccga gatcaccacg ctgtccggcg cattcaccgc cgcgagcgcc agccgatcgg
     1561 agtgctcccg cagcgcctcc gccactgcgg cctctggcgc gaacaccgcg gccatcgctc
     1621 cgcccgcggg cagcgcctgc atcatgcggc cccggatcgc gatcagcccg agcgcgtcct
     1681 ccagcgtcat cagccccgcc acgcaggccg ccacgtactc gcccacgctg tgcccgagca
     1741 cgaagctggg cgtcaccccc cattcgcgcc acgtctcgga cagggcgtac tccagcgcga
     1801 acagagccgg ctgcgtgtac tgtgtctcgt cgaggagcgc gggcgtcccc gcctccccga
     1861 acatcaccga cagcagtggc tcggacaggt gaggtcgaag cagcgagtcg cagcgatcga
     1921 ggatccggcg gaacgtctcg tggcgccggt agagctcgcg acccataccg acgtactggg
     1981 ctccctggcc ggtgaacagg aacgccaccc gtggccgggg ggagcgcgcg cggccgcgat
     2041 gcgcgcgtgc ggtcgcctcg cccgccgcga gccgggcgag ggtctcgcgc gcctccgcgg
     2101 ccgagtggac gaccgccgcg agccggtgcg ggagctgcgc gcggccggtc gccgcggtga
     2161 agcacacgtc gccgagatcg cccggggttt cggcgaggtg ccgctcgtag cggccggcga
     2221 gc
//
LOCUS       DATMCV010000145         4837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_408743, whole genome shotgun sequence.
ACCESSION   DATMCV010000145 DATMCV010000000
VERSION     DATMCV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408743"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VNU03_03505"
     CDS             complement(<1..416)
                     /locus_tag="VNU03_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP73546.1"
                     /translation="MSTLKDSISQAVDRLADELDALSHQIHAHPELAYQEVQACGWLS
                     DFLARQGFKVEKGVGGVETAFRGTIETGEGPTIAILCEYDALPGIGHACGHNVIATSG
                     VGAGAALAAVRAQLPKGRIQVIGTPAEEGGGGKILML"
     gene            complement(689..1984)
                     /locus_tag="VNU03_03510"
     CDS             complement(689..1984)
                     /locus_tag="VNU03_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP73547.1"
                     /translation="MARVSNRGGTTSLIRHLQILRTVTEAVSRSLDLNEVIQKSLSAL
                     TDVTGHEIASLHLVSVDGKMLLLRGERGLSDRLRDTNQMLPMGQGLIGRVAASGRVRR
                     VDEASRAPDLLPAARAAVSADGIRGFVCVPIRARHRILGTLSLGRQTEERFSNEEVAL
                     LESTADQIGLALDNARLYSETRLQLEDLRRAHTDVIRAERLAAVGELAGGVAHEINNP
                     LMIILGQVHLLLQSQDHESVLNGLKVIDGATKRAANIVRELVLFAERSPLRRARCKVS
                     DQIYKVLSLHQSRLEGARITVQTDFGEVPEIWADSNQLQEALFHLVQNAEQAMSTARG
                     SGTLAIRVRPAADGVRIEVADDGPGVPQDDLARVFNPFFTTKGPGEGRGLGLSVAHSV
                     VAEHEGRLWAENRPGGGAMFVMELPVGSPAPDPALAPSA"
     gene            complement(2149..3273)
                     /locus_tag="VNU03_03515"
     CDS             complement(2149..3273)
                     /locus_tag="VNU03_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWP73548.1"
                     /translation="MSPTEAPADARARAYHRRQLALSLAELALTASYLIVLVETRAAV
                     HLAERLATWTPRWWLALAVAVLVLGGGYRLLSLPLAWLGGFWLPRRYGLLHQSLGRWL
                     WDAAKTALIGGALGLVAVEVVYGLMRWSAWWWLWGAVVFLGGYALLALVAPVWLVPLF
                     YRLTPLADGPLKSRLLALAARVGVPVTGVWVVDQSRKSRTANAAVTGLGRTRRILLFD
                     TLLDTFTADEVEAVLAHELAHQLHGDIRRGLLVQGALTLVTFWVASGALRAGGVWLGL
                     DGPADIGGLPLFGLVLLAVGLAALPLANGWSRHVEHAADRFALETLPGPGPFIGAMER
                     LATLNLAERDPHPVEEFFLYSHPPIGRRIAFARQFPGGLT"
     gene            3363..4277
                     /locus_tag="VNU03_03520"
     CDS             3363..4277
                     /locus_tag="VNU03_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWP73549.1"
                     /translation="MFQSVEDVQQQFKNARYIANRRISTVVFLAERMGRPVLIEGPAG
                     VGKTELAKTLAEVTSRHLIRLQCYEGLDEGKALYEWKYAKQLLYTQLLRERIGELIAD
                     APSLPDAVARISGQDDAFFSHRFLAPRPLLQAIESVDPVVLLVDEIDKAEPEFEAFLL
                     EVLSDFQVSVPELGTIRATQIPLVVLTSNNARELSDGLKRRCLHLFIDFPPADEELAI
                     IRLKVPEVSERLAQTVVTAVQRIRGLELRKAPSISESLDWARSLVILNAETLDRDLVE
                     STLTMLVKHEKDLDRVKGALDKVLADLG"
     gene            4284..>4837
                     /locus_tag="VNU03_03525"
     CDS             4284..>4837
                     /locus_tag="VNU03_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73550.1"
                     /translation="MSGSWSALTEFRELWFPSPRLRLPELGPAGPVFIEGGRCISGEL
                     GEYTKLWISAAGRWHFYYIHPSPRDFYRVTMPEAVPHLLHPGEMTVETFRQGLSYKVV
                     LGLRCSEGPVRVLHLVGDVADVTRAEERHEDAPAGPLGWKLSMTHLIAQGSFHGLIRL
                     EDGRLYPITRDGGEDVFKSSWVHFA"
BASE COUNT          730 a         1680 c         1749 g          678 t
ORIGIN      
        1 agcatgagga tcttcccgcc gccgccctcc tcggccggag tcccgatgac ctggatgcgc
       61 cccttcggga gctgggcgcg gaccgcggcg agcgccgcgc ccgcgccgac gccggaggtg
      121 gcgatcacgt tgtgcccgca cgcgtgcccg ataccgggca gggcgtcgta ctcgcacagg
      181 atcgcgatcg tcgggccctc tccggtctcg atggtgccgc ggaacgcggt ctccacgccc
      241 cccacgccct tctccacctt gaagccctgc cgggccagga agtcgctgag ccagccgcac
      301 gcctggacct cctgataggc cagctccggg tgggcgtgga tctggtggga gagcgcgtcg
      361 agctcatcgg ccaggcggtc gacggcctgc gagatggaat ccttgagggt gctcatgggt
      421 gcctccaggc tcagggcgac tgggcgcaga cgaggaagaa gagtggccgg gacgagtcgg
      481 acgaatagcc ccgccccggg tcgttcaggc accgcaggct cggcgacgag tccgaggagc
      541 gtcgcgggga gtccatggtg gcgcaggagg ccaggacgag agcggcgccg agggccatca
      601 gggcacgacc ggcgaacagg gggggcacga gggagcaggg taccacgagc ccggccggtg
      661 aaggggggcg gcgaagcggg acggcggatc aggcggaggg ggcgagtgcg gggtcggggg
      721 ccgggctacc caccggcagc tccatcacga acatcgcgcc gccgccgggt cggttctcgg
      781 cccagaggcg tccctcgtgc tcggccacca cgctgtgcgc gacggacagg ccgaggccgc
      841 gcccctcgcc gggccccttc gtggtgaaga aggggttgaa gacgcgggcc aggtcgtcct
      901 gggggacgcc ggggccgtcg tcggcgacct cgatccgcac cccgtcggcg gccggccgca
      961 cccggatggc gagggtgccc gagccgcgcg ccgtggacat ggcctgctcg gcgttctgca
     1021 cgaggtggaa gagggcctcc tggagctggt tgctgtcggc ccagatctcg ggcacctcgc
     1081 cgaagtcggt ctggaccgtg atgcgggcgc cctcgagccg cgactggtgg agcgagagga
     1141 ccttgtagat ctggtcgctc accttgcagc gcgcgcggcg caggggcgag cgctccgcga
     1201 agagcacgag ctcgcgcacg atgttggccg cgcgcttggt ggccccgtcg atgaccttga
     1261 gcccgttcag caccgactcg tggtcctggg actgcagcag caggtgcacc tgccccagga
     1321 tgatcatgag cgggttgttg atctcgtgcg cgacgccgcc cgcgagctct ccgaccgcgg
     1381 cgagccgctc ggcccggatc acgtcggtgt gcgcgcgccg gagatcctcg agctgcagcc
     1441 gcgtctcgga gtacagccgc gcgttgtcga gggccagccc gatctggtcg gcggtggact
     1501 cgagcagcgc gacctcctcg ttggagaacc gctcctcggt ttgccggccc agcgagagcg
     1561 tgcccaggat gcgatggcgc gcccggatcg gcacgcagac gaagccgcgg atgccgtcgg
     1621 cggagaccgc ggcgcgcgcg gcggggagga gatcgggggc gcggctggcc tcgtccacgc
     1681 ggcgcacccg gcccgacgcg gcgacgcggc cgatcaggcc ctggcccatc ggcagcatct
     1741 ggttggtgtc gcgcagccga tccgagagcc cgcgctcccc gcggagcagg agcatcttgc
     1801 cgtccaccga gacgagatgg agactcgcga tctcgtgccc ggtcacgtcg gtgagcgcgc
     1861 tcaacgactt ctggatgacc tcgttgaggt cgagcgaccg gctgaccgcc tcggtgaccg
     1921 tgcggaggat ctgcaggtgc cggatgagcg aggtggtgcc gcctcggtta cttactcgcg
     1981 ccattagatc ctgtgggctc cccatgatcg gtgtgtgctg gacggctgct ccgtacttag
     2041 caagccaagt gccatgggtg gctatcgaat aataccatct attccagcta gttagatttg
     2101 ttgcttgaag gggtcagccg ggcagagcgg tacagcccgg acactctgtc aggtcagacc
     2161 acccggaaac tgccgcgcga acgcgattcg acggccgatc ggcgggtgcg agtagaggaa
     2221 gaactcctcg accggatgcg ggtcccgctc ggccaggttc agcgtcgcca gccgctccat
     2281 ggcgccgatg aagggcccgg ggccggggag cgtctcgagc gcgaaccggt cggcggcgtg
     2341 ctcgacgtgg cgggaccagc cgttcgccag ggggagcgcg gccagcccca ccgccagcag
     2401 gacgaggccg aagagcggca ggccgccgat gtccgccggc ccatcgaggc cgagccagac
     2461 cccgcccgcc cgcagcgccc cggacgccac ccagaaggtg acgagggtca gggcgccctg
     2521 cacgagcagg ccccgacgga tgtcgccgtg gagctggtgc gccagctcgt gcgcgagcac
     2581 cgcttcgacc tcgtcggcgg tgaacgtgtc gagcagcgtg tcgaacagga ggatgcggcg
     2641 cgtgcggccg aggccggtca ccgccgcgtt ggcggtgcgg ctcttgcggg actggtcgac
     2701 cacccagacc ccggtcaccg gcacgcccac ccgcgcggcg agcgcgagga gccggctctt
     2761 gagcggaccg tcggccagcg gggtcaggcg gtagaagagc ggcacgagcc acaccggtgc
     2821 gaccagggcc agcagcgcgt accctccgag gaacaccacc gctccccaca gccaccacca
     2881 cgcgctccag cgcatgaggc cgtacacgac ctcgaccgcg accaggccca gggccccacc
     2941 gatcagggcg gtcttcgcgg cgtcccagag ccagcgaccg agcgattgat gcaggaggcc
     3001 gtagcgtcga gggagccaga agccgccgag ccaggccagc ggcaggctca ggaggcggta
     3061 gccgccaccc agcaccagga ccgccaccgc gagggcgagc caccagcgcg gcgtccaggt
     3121 cgcgagccgc tcggccaggt gcaccgccgc gcgtgtctcg acgaggacga tcaggtagct
     3181 cgcggtgagg gcgagctcgg cgagcgacag cgcgagctgt cggcggtggt aggcgcgggc
     3241 ccgcgcgtcc gcgggcgcct cggtcgggct catgagtcga gtgtcccacc gttcgggagg
     3301 cccgccaacc ggggtgcccg tctgtgctac actgtgcgcc aattcacaac cagggagccg
     3361 ccatgtttca gtccgtcgag gacgtccagc agcagttcaa gaacgcccgc tacatcgcca
     3421 atcgccggat ctccaccgtg gtgttcctgg ccgagcgcat gggccggccg gtgctcatcg
     3481 agggcccggc gggagtgggc aagaccgagc tggccaagac gctggccgag gtgacctcgc
     3541 ggcatctcat ccgcctgcag tgctacgagg gcctcgacga gggcaaggcg ctctacgagt
     3601 ggaagtacgc caagcagctc ctctacaccc agctgctgcg cgagcgcatc ggcgagctga
     3661 tcgcggacgc cccgtcgctg cccgacgcgg tggcgcggat ctctggccag gacgatgcgt
     3721 tcttctccca ccgctttctc gccccgcgcc cgctcctgca ggcgatcgag tccgtggatc
     3781 cggtggtgct cctggtcgac gagatcgaca aggcggagcc cgaattcgag gcgtttctcc
     3841 tcgaagtcct gtccgacttc caggtctcgg tcccggagct cggtacgatc cgggccaccc
     3901 agatcccgct ggtggtgctc acctcgaaca acgcgcgcga gctctccgac ggcctcaagc
     3961 ggcggtgcct ccacctcttc atcgacttcc cgcccgccga cgaggagctc gccatcattc
     4021 gcctcaaggt ccccgaggtc tcggagcggt tggcccagac ggtggtgacc gcggtgcagc
     4081 gcatccgcgg gctcgagctg cgcaaggccc cgtccatctc ggagagcctc gactgggccc
     4141 gctcgctcgt catcctgaac gcggagaccc tcgaccgcga tctggtggag tcgaccctca
     4201 ccatgctcgt caagcacgag aaggatctcg accgggtcaa gggcgccctc gacaaggtgc
     4261 tggccgacct gggctagtcg cccatgagcg ggagctggtc cgccctcacc gagttccgcg
     4321 agctgtggtt cccgagcccg cgtctccggc tgcccgagct cggccccgcg gggcccgtct
     4381 tcatcgaagg gggccgctgc atctcgggcg agctcggcga gtacaccaag ctctggatct
     4441 cggcggcggg gcgctggcac ttctactaca tccacccgtc gccgcgggac ttctaccgcg
     4501 tgacgatgcc ggaggcggtg ccgcacctgc tgcaccccgg cgagatgacg gtggagacct
     4561 tccgccaggg gctctcctac aaggtggtcc tcggcctgcg ctgcagcgag ggaccggtgc
     4621 gcgtgctgca tctggtgggc gacgtcgcgg acgtcacccg cgcggaggag cggcacgagg
     4681 acgcccccgc gggtccgctc ggctggaagc tctcgatgac ccacctgatc gcgcagggct
     4741 cgttccacgg cctcatccgg ctggaggacg gccgcctgta tccgatcacc cgcgacggcg
     4801 gcgaggacgt cttcaagtcc tcctgggtcc acttcgc
//
LOCUS       DATMCV010000146         2851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_142370, whole genome shotgun sequence.
ACCESSION   DATMCV010000146 DATMCV010000000
VERSION     DATMCV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142370"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VNU03_03530"
     CDS             complement(<1..905)
                     /locus_tag="VNU03_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HWP73551.1"
                     /translation="MPPETFRIQVPDPVLADLRERLARVRWPDQAPGAEWAFGTSLAY
                     MKDLVAHWKDRYDWRAHEARLNAFRQFKAKVAGIDLHFIHEEGRGPRPLPLLLSHGWP
                     GSVWEFHKILPMLTDPARFGGDPADSFTVIAPSLPGYGFSFAPGQPRFGVAEIADAFA
                     ALMTDVLGYGRFAAQGGDWGGFITSRLGATHPERLVGIHVNLLSLRRDIKPPPQPNPE
                     EKQYLEDLGRWQREETGYQWIQGTKPQTLAFGLSDSPAGLAAWIVEKFRTWSDCGGDV
                     ERRFTKDELLTNVMIYWVTGTINAS"
     gene            complement(905..2587)
                     /locus_tag="VNU03_03535"
     CDS             complement(905..2587)
                     /locus_tag="VNU03_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWP73552.1"
                     /translation="MFDPEAIKRQASEKAAWEAKELAEFLARQPEARPTYRTGSGLPV
                     QRIYTAEDIAGAPADNIGLPGRYPYTRGPYPTMYRGRNWTMRQIAGFGTGEDTNRRFR
                     YLIEQGQTGLSVDFDMPTLMGYDSDDPRSLGEVGREGVAIDTLDDMEALFADVDLERI
                     SVSMTINPTAWILLAMYVALAESRGFDPGRLSGTVQADILKEYVAQKEWIFPIRPSMR
                     IMRDMIVWSARHLARYNPINISGYHISEAGATSVQELAFTMANAIAYVEEVTRAGVPV
                     DEFAPRLAFYFVAQADFFEEVAKFRAARRIYARLMKERFGATKAESMRLRFHCQTAAA
                     TLTKAQPLNNVIRTTLQALSAVLGGAQSLHTNGMDEAYAIPSEEAMKIALRTQQVIAL
                     ETSVTNVVDPLGGSYYVEALTNRVEAEVLAILAKVDALGGTIKAIEEGFFQREIADSA
                     YDFARRKAAGEQSVIGVNTLVEPSAPPPIPIHKVDPVVESRQIQRLAEVRRRRDQALV
                     ARLLDRLEREAREPATNLMPVTVELVKARASLGEIVARLRGVFGSYIERPVF"
BASE COUNT          399 a          940 c         1024 g          488 t
ORIGIN      
        1 aaggacgcgt tgatggtgcc ggtcacccag tagatcatca cgttggtcag cagctcgtcc
       61 ttggtgaacc gccgctccac gtcgccgccg cagtcgctcc acgtccggaa cttctccacg
      121 atccacgccg cgagcccggc cggggagtcg ctcagaccga aggccagcgt ctggggcttg
      181 gtgccctgga tccactggta gccggtttcc tcgcgctgcc accgcccgag atcctcgagg
      241 tactgcttct cctcgggatt gggttggggg ggcggcttga tgtcgcggcg gagcgagagc
      301 aggttgacgt ggatgcctac cagccgctcc gggtgggtcg cgccgagccg ggaggtgatg
      361 aagccccccc agtcgccgcc ctgcgcggcg aagcgcccgt agcccagcac gtcggtcatg
      421 agggcggcga aggcgtcggc gatctcggcc accccgaagc gcggctgccc gggcgcgaag
      481 gagaagccgt agccgggtag cgacggagcg atcacggtga acgagtcggc ggggtcgccg
      541 ccgaagcgcg cggggtcggt cagcatcggg aggatcttgt ggaactccca caccgagccg
      601 ggccagccgt gggagagcag cagcggcagt ggccggggcc cgcggccctc ctcgtggatg
      661 aagtggagat cgatcccggc caccttcgcc ttgaactgac ggaacgcgtt cagccgcgcc
      721 tcgtgcgcgc gccagtcgta gcggtccttc cagtgggcga ccaggtcctt catgtaggcg
      781 aggctcgtgc cgaacgccca ctcggcgccc ggggcctggt cgggccagcg cacgcgcgcg
      841 aggcgctcgc gcaagtcggc gagcaccggg tcgggcacct ggatgcggaa cgtctcgggc
      901 ggcatcagaa caccggcctc tcgatgtagg agccgaacac cccgcgcagc cgcgcgacga
      961 tctcgcccag cgacgcgcgc gccttcacga gctccacggt gaccggcatg agattggtgg
     1021 ccggctcgcg ggcctcccgc tccaggcgat cgagcaggcg cgcgacgagg gcctgatcgc
     1081 ggcggcgccg cacctccgcg aggcgctgga tctggcgcga ctcgacgacg gggtccacct
     1141 tgtggatcgg gatcgggggc ggggccgacg gctcgaccag ggtattgacg ccgatcaccg
     1201 actgctcgcc cgccgccttg cgccgggcga agtcgtaggc ggagtcggcg atctcccgct
     1261 ggaagaagcc ctcctcgatc gccttgatgg tgccgccgag cgcgtccacc ttggcgagga
     1321 tcgcgagcac ctcggcctcc acccggttgg tgagcgcctc cacgtagtag gagccgccga
     1381 gcggatcgac cacgttggtc acgctggtct cgagcgcgat gacctgctgg gtgcgcaggg
     1441 cgatcttcat cgcctcttcc gaggggatcg cgtaggcctc gtccatgccg ttggtgtgga
     1501 gcgactgggc cccgcccagc accgcggaga gggcctggag cgtggtgcgg atgacgttgt
     1561 tgagcggctg cgccttggtc agcgtggccg ccgcggtctg gcagtggaag cgcaggcgca
     1621 tcgactcggc cttggtggcc ccgaagcgct ccttcatgag ccgggcgtag atgcggcggg
     1681 ccgcgcggaa cttcgcgacc tcctcgaaga agtcggcctg ggcgacgaag tagaaggcca
     1741 ggcgcggggc gaactcgtcg accgggacgc ccgcgcgcgt cacctcctcc acgtaggcga
     1801 tcgcgttggc catggtgaag gccagctcct gcaccgacgt ggctcccgcc tccgagatgt
     1861 gatacccgct gatgttgatc gggttgtagc gcgcgaggtg gcgcgcgctc cacacgatca
     1921 tgtcgcgcat gatccgcatc gacggccgga tcgggaagat ccactccttc tgggccacgt
     1981 actccttgag gatgtcggcc tgcaccgtcc cggagaggcg gcccgggtcg aagccgcgcg
     2041 actccgccag cgccacgtac atggcgagca ggatccaggc ggtgggattg atcgtcatcg
     2101 acaccgagat gcgctcgagg tcgacgtcgg cgaagagagc ctccatgtcg tcgagggtgt
     2161 cgatggccac cccctcgcgc ccgacctcgc cgagcgagcg cgggtcgtcg gagtcgtagc
     2221 ccatcagggt cggcatgtcg aagtcgacgc tgaggccggt ctgtccctgc tcgatcaggt
     2281 agcggaagcg gcggttggtg tcttccccgg tgccgaagcc tgcgatctgg cgcatcgtcc
     2341 agttgcggcc gcggtacatg gtggggtagg gcccgcgcgt gtacgggtag cggccgggca
     2401 gcccgatgtt gtcggcgggc gcgcccgcga tgtcctccgc ggtgtagatc cgctgcaccg
     2461 gcagccccga gccggtccgg tacgtcggcc gcgcctccgg ctggcgggcg aggaactcgg
     2521 ccagctcctt ggcctcccac gccgccttct cggatgcctg tcgcttgatc gcttccgggt
     2581 caaacatggg ggagcccttt ctgtctttca tctcgcttga acgtggatga cgtcggtttc
     2641 gcggttcggc tccttcgcgc aagcgttttt catctcgctt gaacgtggat gacgtcggtt
     2701 tggcggtttg gctccttcgc gcaagcgttt ttcatctcgc ttgaacgtgg atgacgtcgg
     2761 tttggcggtt cggctccttc gcgcaagcgt ttttcatctc gctcgaacgt ggatgacgtc
     2821 ggtttggcgg ttcggctcct tcgcgcgagc g
//
LOCUS       DATMCV010000147         3020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_740972, whole genome shotgun sequence.
ACCESSION   DATMCV010000147 DATMCV010000000
VERSION     DATMCV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740972"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VNU03_03540"
     CDS             complement(<1..515)
                     /locus_tag="VNU03_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73553.1"
                     /translation="MTRARFTLAVLVALIVASAAGLAAPAPAAAQKPGGTLTIVRPTD
                     PVSLDPQLETTAPGAWVYFNMLEPLLTMDEKMQIRPALAASYEVMSPTKVRFKLRPGV
                     KFHDGTPLNAAAVKFTFERALRGNPPARWASLAGPLTGAEVVDDLTVDVTTREPYGPI
                     LRTLAMYCTGIV"
     gene            complement(512..1546)
                     /locus_tag="VNU03_03545"
     CDS             complement(512..1546)
                     /locus_tag="VNU03_03545"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWP73554.1"
                     /translation="MAITVYGAGAIGGTTGAALARAGHDVLLVDSATPHVEAINAHGI
                     TIERRDGVTTTRVPAVTPDGLGRDLALVLLAVKSHHTADALRDLAPRLAPAGTIVSLQ
                     NGLSEEQIAAAVGVQRTIGCLVNWAADWVAPGRILHGGHGAFVLGELDGRDTPRVREL
                     AALLSAVEETPVTDNIWGYKWAKLIYGALLFATAVVDAHVYEVVERSPGVKDALVGIV
                     GEGIAVADKAGVRVLAFDEFDPAWYRAALAGDVAARDRAMAAIAAHYRQHTKTKTGIW
                     RDLAVRKRKTEVDGQLGMLARKGEALGVAMPLTRRLAELIRDLEDGRRQMDWSNLDPL
                     VTLAEKETRS"
     gene            1661..2236
                     /locus_tag="VNU03_03550"
     CDS             1661..2236
                     /locus_tag="VNU03_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP73555.1"
                     /translation="METVQMSREEIESRTGRFTALQPMEFMKNDTTVSQAAKDIILAR
                     KLMPVVLERTQSAFGNDAPIYNAAGLTMFISIMPPGQGPCLHAHVKTFETFIVLEGKL
                     EFSLNDDGGEKIVLNRWDTFSCPPGVNRGFRNVDTKDSVLLTVITGGINDRNDIGLPP
                     AIARQIEAVDPHALEVFKTKMGLRFDAGPEV"
     gene            complement(2378..>3020)
                     /locus_tag="VNU03_03555"
     CDS             complement(2378..>3020)
                     /locus_tag="VNU03_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP73556.1"
                     /translation="EKRLNHYRRHGVPEASVEAFRGRGNHFRQLVPTVAPEFRSVREG
                     DVVTIGRRAWHAFTVMGHAPEHACLFSPEANVLISGDQVLPKITTNVSVWPDQPNGNP
                     LKLYLDSLGRFRPLPESTLVLPSHGLPFQGLHRRLDYLQHHHDERLSEAIDALAEPRT
                     ANDLVPVLFRRQLDAHQLGFAIGETVAHLHFLEARGQASRLVGDDGVHRFRKA"
BASE COUNT          437 a         1085 c         1070 g          428 t
ORIGIN      
        1 acgatgccgg tgcagtacat cgcgagggtg cggaggatgg gtccgtaggg ctcgcgcgtg
       61 gtcacgtcga cggtgagatc gtcgaccacc tccgcgcccg tgagcgggcc ggcgaggctc
      121 gcccaccgcg cgggcgggtt gccgcgcagc gcgcgctcga aggtgaactt caccgccgcc
      181 gcgttgagcg gggtaccgtc gtggaacttg acccccggcc gcagcttgaa gcgcaccttg
      241 gtcggcgaca tcacctcgta ggaggcggcc agcgcggggc ggatctgcat cttctcgtcc
      301 atggtgagca gcggctcgag catgttgaag tacacccacg ccccgggcgc ggtggtctcg
      361 agctggggat cgagcgagac cgggtcggtg gggcgcacga tggtgagcgt gccgcccggc
      421 ttctgggccg cggcgggggc cggggcggcg agcccggccg cgctcgccac gatgagggcc
      481 accaggacag ccagcgtgaa ccgggcacgc gtcatgagcg ggtctccttt tccgcgaggg
      541 tcaccagcgg gtccagattg ctccagtcca tctgccgccg gccgtcctcc agatcgcgga
      601 tcagctcggc gagccggcgg gtgagcggca tcgccacccc gagcgcctcg cccttgcggg
      661 cgagcatgcc gagctggccg tccacctcgg tcttccgctt gcgcaccgcg aggtcgcgcc
      721 agatgccggt cttggtcttg gtgtgctgcc ggtagtgggc cgcgatggcg gccatcgcgc
      781 ggtcgcgggc cgcgacgtcg cccgcgaggg ccgcgcggta ccacgcggga tcgaactcgt
      841 cgaaggccag gacgcgcacg cccgccttgt cggcgaccgc gatcccctct ccgacgatcc
      901 ccaccagcgc gtccttcacg cccgggctcc gctccaccac ctcgtagacg tgggcgtcca
      961 ccaccgcggt cgcgaagagc agcgcgccgt agatgagctt cgcccacttg tagccccaga
     1021 tgttgtcggt gaccggggtc tcctcgaccg cgctgaggag cgcggccagc tcgcgcaccc
     1081 gcggggtgtc tcgcccgtcg agctcgccca gcacgaaggc cccgtgcccg ccgtggagga
     1141 tgcggccggg cgcgacccag tccgcggccc agttgaccag gcagccgatg gtgcgctgga
     1201 cgccgacggc ggccgcgatc tgctcctcgc tcagcccgtt ctggagcgag acgatggtgc
     1261 cggcgggggc gagccgcggc gcgaggtcgc ggagcgcgtc ggcggtgtgg tggctcttca
     1321 ccgcgagcag gaccagcgcg aggtcgcggc ccagcccgtc cggcgtgacc gcgggcaccc
     1381 gggtggtggt gaccccgtcc cggcgctcga tcgtgatgcc gtgggcgttg atcgcctcca
     1441 cgtggggcgt ggcgctgtcg accaggagga cgtcgtggcc ggcccgcgcg agggctgcgc
     1501 cggtggtgcc tccgatcgcg ccagccccgt agaccgtgat cgccatgggc ccctcaagta
     1561 ccacagggcc cggagggcgt caacgcggga cgggtaccgc gcccgccccc ggccgtgcta
     1621 tggtccagtg ctccagatcg cgccacccca ggaggaccgc atggagaccg tgcagatgag
     1681 ccgggaggag atcgagagcc gcaccgggcg cttcaccgcg ctccagccca tggagttcat
     1741 gaagaacgac accaccgtct cgcaggcggc gaaggacatc atcctcgcgc gcaagctgat
     1801 gccggtggtg ctggagcgga cccagtccgc cttcggcaac gacgcgccga tctacaacgc
     1861 ggccggcctc accatgttca tctcgatcat gccgcccggc cagggcccct gcctgcacgc
     1921 ccacgtgaaa accttcgaga ccttcatcgt gctggagggc aagctcgagt tctcgctcaa
     1981 cgacgacggg ggcgagaaga tcgtgctgaa ccgctgggac acgttctcgt gcccgcccgg
     2041 ggtcaatcgc ggcttccgca acgtggacac caaggacagc gtgctgctca ccgtcatcac
     2101 cggcggcatc aacgaccgca acgacatcgg gctcccgccc gccatcgcgc ggcagatcga
     2161 ggcggtggac ccgcacgcgc tcgaggtgtt caagaccaag atgggcctgc ggttcgacgc
     2221 gggcccggag gtctgagacg gcacctcacc cctccaccat ccctaacgct ccaccacccc
     2281 tcaccctcca ccacccctca ccctccacca cccctcaccc tgccctctcc cctgagggga
     2341 gagggggatg aggaggccgg aggcaccttt gagcggctca ggccttccgg aagcggtgga
     2401 ccccgtcgtc gcccacgaga cggctcgcct gcccccgcgc ctcgaggaag tgcaggtggg
     2461 ccaccgtctc gccgatcgcg aagccgagct ggtgggcgtc gagctgccgg cggaagagca
     2521 ccggcaccag gtcgttcgcg gtgcgcggct ccgcgagcgc gtcgatcgcc tcgctgagcc
     2581 gctcgtcgtg gtggtgctgc aggtagtcga gccgccggtg caggccctgg aacggcaggc
     2641 cgtgggacgg gagcaccagg gtcgactcgg gtagcggacg gaagcggccg agcgagtcca
     2701 ggtagagctt gagcgggttg ccgttcggct gatccggcca cacgctcacg ttggtggtga
     2761 tcttgggcag cacctggtcg cccgagatca gcacgttggc ctccggactg aagagacagg
     2821 cgtgctcggg cgcgtggccc atcaccgtga acgcgtgcca cgcccgtctg ccgatggtga
     2881 ccacgtcccc ctcacgcacc gagcggaact ccggcgcgac cgtcggcacg agctggcgga
     2941 aatggttccc ccgcccgcgg aaggcctcca ccgacgcctc cggcaccccg tggcgccggt
     3001 agtggttgag ccgcttctcg
//
LOCUS       DATMCV010000148         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_804903, whole genome shotgun sequence.
ACCESSION   DATMCV010000148 DATMCV010000000
VERSION     DATMCV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804903"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VNU03_03560"
     CDS             complement(<1..636)
                     /locus_tag="VNU03_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73557.1"
                     /translation="MLRLCLTRCLAGIPALLLVTVLVFTVVRLIPGDPARILAGDFAT
                     DQAVAELRQRWRLDRPLPVQYAAYVAGLARGDLGRSTATSLPVAGELGERFLRTLLLA
                     GAAILVATLVGVTLGILSATHRASLLDFAATALALAGVSTPIFWSGLILILLFSVELP
                     WLPAGGTGSFAHLILPAVSLGLFGAGVIARQTRSSMLETLGEDFVRTARAKG"
     gene            complement(658..2184)
                     /locus_tag="VNU03_03565"
     CDS             complement(658..2184)
                     /locus_tag="VNU03_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73558.1"
                     /translation="MRVLATLLALIALLLAPPGPAQAGKDTLTIGMPSDVPIFDTHKA
                     TGLYNGSILNQVSEPLVLLSTKGEAQPWLAESWEQSKDGKSWTIKLRKGVKFTDGTPL
                     TAEVVKFSLERFRKHSIGKATLAIVETITAVDEHTLRLTTKAPYAPMINTLGYHWIVV
                     YSEAQIKKVGDDNLHTAPVGSGPFKLVHHKRGQEVRLEANDQYWAGKPRLRTVISRPY
                     PDLSARVLALESGDVDLIFQVPPQEAARLAKNPNLTVFTPPTARVIWMYLNTQKEPFK
                     DKRVRQALYHAIDRAAIVKSIFAGTAPPLHSPGPVGSYGYTEAHDHYGFDPEKARRLL
                     REAGQPAITFTVHHTPGRYLLSGQVLEAIQGYLKQVGVTMKILDLEWGTLSQQAALPV
                     DRNPVQAAFFGWRSVNGDIDSAIQDFGSQFWRPAGNNLSFYKNEEFDRLLEQEQGALD
                     PKKRLAALSRMQEILMEDAPAIFLYGEPQIWAARKTLKGLDWNPLESLYPLHRAYFDD
                     "
     gene            complement(2314..>2767)
                     /locus_tag="VNU03_03570"
     CDS             complement(2314..>2767)
                     /locus_tag="VNU03_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73559.1"
                     /translation="PLGSRTPSADPSRHTMELLAKHGFVYHSNALDTDLPYAVQTRHG
                     PLVEFPTAWCNNDAPFFMWSSVPPVGNGIWSQQDVWEIWSEEFEGLYAEGGFFNWLAH
                     PQIIGRTSRMRMVERLIQLILGKGRVWWPKPIDLARFWLQKTAAAGRA"
BASE COUNT          405 a          916 c         1034 g          412 t
ORIGIN      
        1 gcccttggcc cgcgcggtgc gcacgaagtc ctccccgagc gtctcgagca tgctcgagcg
       61 ggtctgccgc gcgatcaccc cggccccgaa gaggccgagg ctcaccgcgg gcaggatcag
      121 gtgggcgaag ctgccggtgc cgccggcggg cagccagggc agctcgaccg agaagagcag
      181 gatcaggatc agcccggacc agaagatggg ggtggagacg ccggccagcg cgagcgcggt
      241 ggccgcgaag tcgagcaggc tggcgcgatg ggtggcgctc aggatgccga gggtcacccc
      301 gaccagcgtg gccaccagga tcgccgcgcc ggccagcagg agcgtgcgga ggaaccgctc
      361 gcccagctcg cccgccaccg gcagcgaggt cgcggtggag cggccgaggt cgccgcgggc
      421 gagcccggcc acgtacgccg catactgcac cggcagcggg cgatcgagcc gccagcgctg
      481 gcgcagctcg gccaccgcct ggtcggtggc gaagtcgccg gccaggatgc gtgcggggtc
      541 gccggggatc aggcgcacca ccgtgaagac gagcaccgtg accaggagca gcgcgggaat
      601 gccggccagg caacgcgtca ggcagaggcg cagcatcgcg agacccggcg ggcgcgctca
      661 gtcgtcgaag taggcgcggt gcagggggta gagcgattcg agcgggttcc agtcgagtcc
      721 cttgagggtc ttccgcgccg cccagatctg gggctcgccg tagaggaaga tcgcgggggc
      781 gtcttccatc aggatctcct gcatgcggga gagcgcggcg aggcgcttct tggggtcgag
      841 cgcgccctgc tcctgctcga gcagccgatc gaactcctcg ttcttgtaga aggagagatt
      901 gttgccggcc ggacgccaga actgcgagcc gaagtcctgg atcgcgctgt cgatgtcgcc
      961 gttcaccgag cgccagccga agaacgcggc ctgcaccggg ttccggtcca ccggcagggc
     1021 cgcctgctgc gagagcgtgc cccactcgag gtccaggatc ttcatggtca cgccgacctg
     1081 cttgagatag ccctggatcg cctcgagcac ctgcccgctc aggagatagc ggcccggggt
     1141 gtggtgcacc gtgaaggtga tcgcgggctg gcccgcctcg cgcaggagcc gccgggcctt
     1201 ctccggatcg aagccgtagt ggtcgtgggc ctcggtgtag ccgtagctgc ccaccggccc
     1261 cggcgagtgc agtggcggcg cggtccccgc gaagatgctc ttcacgatcg cggcccggtc
     1321 gatggcgtgg tagagcgcct gccgcacccg cttgtccttg aacggctcct tctgggtgtt
     1381 gaggtacatc cagatcaccc gcgcggtggg cggggtgaac acggtgaggt tgggattctt
     1441 ggccaggcgc gcggcttcct ggggcggcac ctggaagatc aggtccacgt cgccgctctc
     1501 gagcgcgagc acccgcgcgg agaggtccgg gtagggccgg ctgatcacgg tccggagacg
     1561 cggcttgccc gcccagtact gatcgttggc ctcgagccgc acctcctgcc cgcgcttgtg
     1621 gtggacgagc ttgaacggcc cgctccccac cggggcggtg tgcaggttgt cgtcgcccac
     1681 cttcttgatc tgggcctcgc tgtacaccac gatccagtga tagccgaggg tgttgatcat
     1741 cggcgcgtac ggcgccttgg tggtgaggcg cagggtgtgc tcgtccaccg cggtgatggt
     1801 ctcgacgatg gcgagggtgg ccttgccgat cgaatgcttc cggaagcgct ccaggctgaa
     1861 cttcaccacc tcggcggtca ggggcgtgcc gtccgtgaac ttgaccccct tgcgcagctt
     1921 gatcgtccag ctcttgccgt ccttcgactg ctcccacgac tcggcgagcc acggctgggc
     1981 ctcgcccttg gtggagagga gcaccagcgg ctcggacacc tggttgagga tgctgccgtt
     2041 gtagaggccg gtggccttgt gggtatcgaa gatcgggacg tcgctcggca tcccgatggt
     2101 cagcgtgtcc ttgcccgcct gcgcggggcc gggcggggcg aggagcagcg cgatcagggc
     2161 gagcagggtg gcgaggacgc gcatgccgcc cgcgaggcgc gcgccctccc gcgcgtaggc
     2221 cagcgcgatg gagcggccga agccgcggcc ggcgccggtg atcagcgcga ccttcctgcg
     2281 agacgaccgg ccgggacgga caccggcggg ccctcaggcg cgcccggccg cggcggtctt
     2341 ctgcagccag aaccgggcca ggtcgatggg cttgggccac cacacgcggc ccttgccgag
     2401 gatgagctgg atgagccgct ccaccatccg catccgcgag gtccggccga tgatctgcgg
     2461 gtgggcgagc cagttgaaga agccgccctc cgcgtagagg ccctcgaact cctcggacca
     2521 gatctcccac acgtcctgct ggctccagat gccgttgccg accggcggca ccgagctcca
     2581 catgaagaac ggcgcgtcgt tgttgcacca cgcggtcggg aactccacca gcgggccgtg
     2641 ccgggtctgg accgcgtagg ggagatcggt gtcgagcgcg ttgctgtgat agacgaagcc
     2701 gtgcttggcg agcagctcca tggtgtgccg gctcgggtcg gccgacggcg tgcgcgagcc
     2761 gaggggc
//
LOCUS       DATMCV010000149         3762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_540413, whole genome shotgun sequence.
ACCESSION   DATMCV010000149 DATMCV010000000
VERSION     DATMCV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540413"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VNU03_03575"
     CDS             complement(<1..691)
                     /locus_tag="VNU03_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylammeline chlorohydrolase"
                     /protein_id="HWP73560.1"
                     /translation="MTKTVCVKNADWVVAWDAASGSHAYLERGDVAFSGGTLTFVGRG
                     YPGRADETIDGRGLMVMPGLIDVHSHPCLEASYRGIREEHGVPEMYMSGLFERAGAYW
                     PDDDGKLACAEAAYCELLRSGVTTLVDISGDYPGWIDLIARSGLRGVLAPAYASSRWV
                     VRRSHLLEYEWNEAAGRQGFEEALRLCDDLAKHPSGRLTGMISPAQIDTCTEDLLRDS
                     VAAARERGLPFT"
     gene            complement(702..1550)
                     /locus_tag="VNU03_03580"
     CDS             complement(702..1550)
                     /locus_tag="VNU03_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73561.1"
                     /translation="MRASGLRRFARGFFRRWTAPAGLAIFLLVLGMAVSAPYVFREDP
                     FSMVTRPLQWPGESREYLLGSDMLGRDILAGIFHGARVSLAIGGIATAAALGAGVLIG
                     ALAGYYGGVPDDLLMRATEAFQTIPPFVFVIVVVVIARPSIRSIILAIALVSWPAVAR
                     LVRAEFLALRHREFVQGCRAVGMGDLRIIFRQILPNALAPIIVTASIMVATAILTESA
                     LAFLGLGDANVMSWGSMIGAGREMLRTEWYLTAIPGLAILLTVLALNLIGEGLNDALN
                     PRLRDQ"
     gene            complement(1550..2539)
                     /locus_tag="VNU03_03585"
     CDS             complement(1550..2539)
                     /locus_tag="VNU03_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73562.1"
                     /translation="MTLARPGVARFLARRLAQAVPTVLLIIVVNFLVLHLTPGDIVDV
                     LAGEAGAATPEYVAMLRQRFGLDRPLAAQLGSYVWGVVNLDLGFSFRHNTTVLHLVLD
                     RLPATLVLMGTSLGLAVALGVVFGVTAARHVNRPADGVIAVLVLLSYATPTFWIGLML
                     IVLFSVKLGWLPTGGMMTIGAGLSGFGRALDVAHHLIPPAASLSLFYAAIYTRLMRAS
                     MLEVAGQDYVRTARAKGISETRVLYGHALRNALLPLVTVVGVQVGSLLGGSVLVESVF
                     GWPGLGRLAFEAVQQRDYNLLLGILLMSSLLVIAVNLVIDLLYAVLDPRIELR"
     gene            complement(2567..>3762)
                     /locus_tag="VNU03_03590"
     CDS             complement(2567..>3762)
                     /locus_tag="VNU03_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73563.1"
                     /translation="FAKVTAVDTPDASTVVIRLSDPTPMMLSAFSSYESQVLPKHVYE
                     GKDFTTHPALNAPIGTGPFVFKEWKKGDFIRLEKNPSYWDKGKPYLDAIVIRIIPDAA
                     ARAAALEKGEVQLGVFNPVPLSDVKRLAALPTVKAETRGYEYFAPMYMMEVNLRDEHL
                     KDKRVRQAMMHALDRKFIADNIWFGFAKPATGPIVSSSPFYTTQGVPQYPYDVAKANA
                     ILDEAGLKRGAGNMRFKVSLTFPPVQPEIPRTAEYIKQALGRAGIDVELRNIDIATFI
                     RQVYNWEFELTQNYLYLLPDPTLGVQRLYISANIKKGTPFANAAGYENKDVDAAFTAA
                     QTENDQAKRKELFARAQRQIQEDLPAFNLLELSFVTLYNSKLVNHTMGADGPYGSFRD
                     TYFQP"
BASE COUNT          603 a         1215 c         1410 g          534 t
ORIGIN      
        1 ccgtgaacgg cagcccgcgc tcgcgggcgg ccgccacgct gtcgcgcagg agatcctcgg
       61 tgcaggtgtc gatctgggcc ggagagatca tgccggtgag ccggccgctc ggatgcttgg
      121 cgaggtcgtc gcagagccgc agggcctcct cgaacccttg gcggccggcc gcctcgttcc
      181 actcgtactc gaggaggtgg gaccgccgca caacccatcg agacgatgcg taggcggggg
      241 cgagcacccc gcgcagcccg ctccgggcga tgaggtcgat ccagcccggg tagtcgcccg
      301 agatgtccac gagcgtggtg acgccggagc gcagcagctc gcagtaggcg gcttccgcgc
      361 aggcgagctt gccgtcgtcg tccggccagt aggcgcccgc gcgctcgaag aggcccgaca
      421 tgtacatctc gggcaccccg tgctcctccc ggatgccgcg gtagctggcc tcgaggcagg
      481 ggtgggagtg gacgtcgatg aggcccggca tcaccatgag gccgcgcccg tcgatggtct
      541 cgtcggcgcg gcccggatag ccgcggccca cgaaggtgag ggtgccgccg ctgaaggcga
      601 cgtccccacg ctcgagatag gcgtggctgc cgctcgcggc gtcccacgcc accacccagt
      661 ccgcgttctt gacgcacacc gtcttggtca tcgcgtgtct cctactggtc tcgcagtcgg
      721 gggttgagcg cgtcgttcag gccttcgccg atcaggttga gggccagcac cgtcaggaga
      781 atcgccagcc ccgggatcgc ggtgaggtac cactcggtgc ggagcatctc acgcccggcc
      841 ccgatcatcg agccccagct catcacgttg gcgtcgccga gccccaggaa ggcgagcgcc
      901 gactcggtga ggatggcggt ggccaccatg atcgacgcgg tgacgatgat cggggccagc
      961 gcgttgggca ggatctggcg gaagatgatg cgcaggtcgc ccatgcccac cgcgcggcag
     1021 ccctgcacga actcccggtg gcggagcgcg aggaactcgg cgcgcaccag ccgcgccacc
     1081 gcgggccagg agacgagcgc gatggccagg atgatcgagc ggatcgaggg gcgggcgatc
     1141 accaccacca cgatgacgaa gacgaacggg gggatggtct ggaacgcctc ggtggcgcgc
     1201 atcaggagat cgtccggcac gccgccgtag tagccggcga gcgccccgat gagcacgccc
     1261 gcccccagcg ccgccgcggt ggcgatgccg ccgatggcga gggagacccg cgcgccgtgg
     1321 aagatgccgg ccaggatgtc gcggcccagc atgtccgagc ccaggaggta ctcgcggctc
     1381 tcgcccggcc actggagcgg ccgggtcacc atcgagaacg gatcctcccg gaagacgtag
     1441 ggggcgctca ccgccatgcc caggaccagg aggaagatcg cgaggccggc gggcgcggtc
     1501 cagcggcgga agaagccccg ggcgaagcgg cggaggccgg aggcccgcat cagcgcagct
     1561 cgatccgcgg atcgagcacc gcgtagagca ggtcgatcac caggttgacc gcgatcacca
     1621 gcagcgagct catgagcagg atgccgagca gcaggttgta gtcgcgctgc tgcaccgcct
     1681 cgaaggcgag gcggccgagg ccgggccagc cgaagacgct ctccaccagc accgagcccc
     1741 cgaggagcga gcccacctgg acgccgacca cggtcaccag gggcaggagc gcgttgcgga
     1801 gggcgtggcc gtacagcacc cgcgtctcgc tgatgccctt ggcgcgggcg gtgcggacgt
     1861 agtcctggcc cgcgacctcg agcatggagg cccgcatgag gcgggtgtag atggcggcgt
     1921 agaagaggga aagggaggcc gcgggcggga tcaggtggtg cgccacgtcg agcgcgcggc
     1981 cgaatccgga caggccggcg ccgatggtca tcataccgcc ggtcggcagc cagccgagct
     2041 tgaccgagaa cagcacgatc agcatcagcc cgatccagaa cgtcggggtc gcgtaggaga
     2101 gcagcacgag caccgcgatc accccgtcgg ccggccggtt gacgtggcgg gccgcggtca
     2161 ccccgaagac cactccgagc gccaccgcga gcccgaggct cgtgcccatc agcacgaggg
     2221 tggccgggag ccggtccagc accagatgga gcacggtggt gttgtggcgg aaggagaagc
     2281 cgaggtccag gttgacgacg ccccagacgt agctcccgag ctgggccgcg agcgggcggt
     2341 cgagaccgaa gcgctggcgc agcatcgcca cgtactcggg ggtagccgcc cccgcctcgc
     2401 cggcgagcac gtccacgatg tcgccggggg tgaggtgcag caccaggaag ttcaccacga
     2461 tgatgaggag gaccgtgggc accgcctggg cgaggcgccg cgcgaggaac cgtgcgacgc
     2521 ccggtcgggc cagcgtcacc gtcacaccgg acgcggcgcc ggccgcctag ggctggaagt
     2581 aggtgtcgcg gaagctcccg tacggcccgt cggcgcccat cgtgtgattc acgagcttgc
     2641 tgttgtagag ggtgacgaac gacagctcga ggaggttgaa agcgggcagg tcctcctgga
     2701 tctggcgctg ggcccgggcg aacagctcct tgcgcttggc ctggtcgttc tcggtctgcg
     2761 ccgcggtgaa ggccgcgtcg acgtccttgt tctcgtagcc ggccgcgttg gcgaaggggg
     2821 tgcccttctt gatgttggcg ctgatgtaga gccgctggac tccgagcgtg gggtcgggca
     2881 ggaggtagag gtagttctgg gtgagctcga actcccagtt gtagacctgg cggatgaagg
     2941 tggcgatgtc gatgttgcgc agctccacgt cgatgcccgc gcggccgagg gcctgcttga
     3001 tgtactccgc ggtgcgcggg atctcgggct gcaccggcgg gaaggtcaga gaaaccttga
     3061 agcgcatgtt gccggcgccg cgcttgaggc cggcctcgtc gaggatcgcg ttggccttcg
     3121 ccacgtcgta ggggtactgg ggcacgccct gggtggtgta gaagggggac gatgagacga
     3181 tggggccggt ggccggcttg gcgaagccga accagatgtt gtccgcgatg aacttgcggt
     3241 cgagggcgtg catcatcgcc tgccgcaccc gcttgtcctt caggtgctcg tcgcggaggt
     3301 tgacctccat catgtacatg ggggcgaagt actcgtagcc gcgcgtctcc gccttcacgg
     3361 tgggcagcgc ggccaggcgc ttgacgtcgg agagcggcac cgggttgaag acgccgagct
     3421 gcacctcgcc cttctccagc gcggcggccc gggccgccgc gtcggggatg atccggatca
     3481 cgatcgcgtc gagatacggc ttgcccttgt cccagtagct cgggttcttc tccaggcgga
     3541 tgaagtcgcc cttcttccac tccttgaaca cgaacggccc ggtgccgatc ggcgcgttga
     3601 gcgcggggtg ggtggtgaag tccttgccct cgtagacgtg cttggggagc acctgcgact
     3661 cgtaggagct gaacgcggag agcatcatcg gggtggggtc cgacaggcgg atcaccaccg
     3721 tgctggcgtc gggggtatcg accgcggtga ccttggcgaa gg
//
LOCUS       DATMCV010000150         5824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_540428, whole genome shotgun sequence.
ACCESSION   DATMCV010000150 DATMCV010000000
VERSION     DATMCV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540428"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VNU03_03595"
     CDS             complement(<1..624)
                     /locus_tag="VNU03_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73564.1"
                     /translation="MSPRSSASKRSRSRAPAVAPSPPPVPIADVSPTPWWWGLAPLAG
                     ALVTVAVFWPALGHQFLDWDDTMNLVDNPAFRGLGGSHLRWMFTTTLAGHWIPLTWIS
                     FGADYLVWGMNPRGYHLTNVLLHAAAAVTFYFVARRILRAATGVGERFLRLGALAAAL
                     FFAIHPLRVESVAWATERRDVLSGLWFLLTILTYLKAAGAAGARRGGW"
     gene            complement(621..1400)
                     /gene="recO"
                     /locus_tag="VNU03_03600"
     CDS             complement(621..1400)
                     /gene="recO"
                     /locus_tag="VNU03_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWP73565.1"
                     /translation="MALYKTRGVVIGRRAFGESDRLVDFYTRDHGKVRGIARSARRPR
                     SRFGSALELFTLGEMVFFDSGRSELVQVDHFDIVRSFVGVREHLERLGQGAWAVEVVA
                     RLSADRDPQPALFGLLVRALQAMETAARPARVAVCFGLRAVDLLGHRPRIDRCMSCGR
                     LHPFDDPALDVAAGGLVCAACRPGADAIPLSGALVGTLKRLRALSWDEALRLNLASDL
                     DAELAGLLEGLLARLMGRYPLSSRFITQTRRVPPMVSLPER"
     gene            complement(1400..2308)
                     /gene="era"
                     /locus_tag="VNU03_03605"
     CDS             complement(1400..2308)
                     /gene="era"
                     /locus_tag="VNU03_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWP73566.1"
                     /translation="MMPAPHRAGFVALVGRPNVGKSTLLNRLVGEKMAIVSPRPQTTR
                     TRITGIRHLPSAQVIFVDTPGLHPGAGRLGQLMSKTAERAVEDVDLVCFVAEATEDPG
                     RLDREALDRLKVSRAPVFCCLNKTDLVSPKARLLPLIAAYRVAHPFAEIVPISAERGE
                     HCARLLELIVATLPERPAYFPPDALTDQPETFWVAEAIREKIFRLTHQEVPYACAVRV
                     EELTERRRPECLYIRAVIFVERESQRGIVIGKGAAMLKRIGATARTDLERFFGIKVFL
                     DLTVRVRAEWRKDDRALREFGFLLTS"
     gene            complement(2305..2769)
                     /gene="ybeY"
                     /locus_tag="VNU03_03610"
     CDS             complement(2305..2769)
                     /gene="ybeY"
                     /locus_tag="VNU03_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWP73567.1"
                     /translation="MPVAISNLQRRSRVFPGRLRAVARRALAALGRADREVHVTVVDD
                     RRIRALNARYLGTRRATDVLAFDLAGPGPGRLWGEVIVSAETAARHARRLRVPLAVEL
                     DLLVVHGLLHLAGYDDHDPRRARRMHERAREILAGRSTRAVPARLWAGLLEA"
     gene            complement(2784..3770)
                     /locus_tag="VNU03_03615"
     CDS             complement(2784..3770)
                     /locus_tag="VNU03_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWP73568.1"
                     /translation="MTEATGVTRRIVLPPDLNHLALLGRQDEHLRTLETQHDVRITAR
                     GHDITLRGEERQVVEAERVLRELVAMLRERPSLGAADIRSALRIASNEPGGDVKSVLA
                     DSIAVPSRRRFVTPKTANQRRYLEAVRGHDLVIAIGPAGTGKSYLAVAMAVSALLKRE
                     VARIILTRPAVEAGERLGFLPGDLVEKVHPYLRPLYDALYDMLEPEKVGSLSEKGAIE
                     IAPLAYMRGRTLNDAFIILDEAQNTTSEQMKMFLTRLGFNSKMVVTGDITQVDLPASR
                     TSGLIEIQSVLQGIDGIRFIYFDDRDVVRHDLVSSIVRAYDRVRKPPTRGAG"
     gene            complement(3801..5591)
                     /gene="aspS"
                     /locus_tag="VNU03_03620"
     CDS             complement(3801..5591)
                     /gene="aspS"
                     /locus_tag="VNU03_03620"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWP73569.1"
                     /translation="MTESLGGWRRTHSCGALRSEHVGQAVTLMGWAYRRRDHGGLVFV
                     DLRDREGLTQCVFNPGAGGGAHAKAESVRAEFVLAVRGTVGARPAGTENPRLATGAVE
                     VQVTEIRILNESRPLPFQLEDDADIDETLRLKYRYLDLRRPHMLRAFQIRDQVCRTAR
                     DYLHAEAFLEVETPFLTRSTPEGARDFLVPSRLQPGSFYALPQSPQLFKQLLMVAGFE
                     RYFQIVRCFRDEDLRKDRQPEFTQIDIETSFLDRDDFLPIIEGLVAAVWRAVKGVELA
                     LPFPRIAYDEAIGRYGSDKPDLRFGLELVDCSALFAGSEFQAFAQTVAAGGAVKGLRV
                     PGAGGMSRKELDDLTAEAKQAGAKGLVWIKVSPEGVQSPVAKFMTSIQGRLLETLGAT
                     PGDLLLLVADAPMVAATVLGRLRVDLARRFDLIPADRDVFAWVIDFPLVEWNADEKRW
                     DAVHHPFTAPRDEDLPLLESDPGKARAKAYDLVLNGQEAAGGSIRIHQPTVQERVFGL
                     IGISKEEGRARFGFLLDALEFGAPPMGGIAFGLDRLAANLAGQESIREVIAFPKTQKG
                     TCPLTEAPAPVDAKQLRELGIRVVEGLGPA"
     gene            complement(5588..>5824)
                     /locus_tag="VNU03_03625"
     CDS             complement(5588..>5824)
                     /locus_tag="VNU03_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73570.1"
                     /translation="LRVALVFDDTMDLTPVLGLARDLREQGRHVLLETRGKRLGKQLQ
                     DLEARGFRRIGVVGADGTVEWREPRTAGAEETKA"
BASE COUNT          877 a         2090 c         2111 g          746 t
ORIGIN      
        1 ccatcccccg cggcgcgcgc ccgccgcgcc ggccgccttc agataggtca ggatcgtcag
       61 caggaaccag agcccggaga ggacgtcgcg acgctcggtc gcccacgcga ccgactcgac
      121 ccgcagggga tggatcgcga agaagagcgc ggcggcgagc gcccccagcc ggaggaaccg
      181 ctcccccacg ccggtggccg cgcgcaggat ccgccgcgcc acgaagtaga aggtgaccgc
      241 ggcggcggcg tggagcagca cgttggtgag gtgataacct cgcgggttca tgccccagac
      301 cagatagtcg gccccgaagg agatccaggt gagcgggatc cagtggccgg cgagggtggt
      361 cgtgaacatc cagcgcagat ggctcccgcc gagcccgcgg aacgcgggat tgtcgacgag
      421 attcatggtg tcgtcccagt cgaggaactg atggccgagg gccggccaga acaccgcgac
      481 ggtgaccagc gcgccggcga gcggggcgag cccccaccac cacggagtcg gggagacgtc
      541 ggcgatcggg accggagggg gcgagggcgc gaccgccggg gcgcgcgatc gggagcgctt
      601 cgacgccgac gacctcgggc tcaccgctcg ggcagcgaca ccatcggagg cacgcgtcgc
      661 gtctgggtga tgaagcgcga cgagagcggg tagcgcccca tgagccgcgc gaggagcccc
      721 tcgagcaggc cggccagctc cgcgtcgagg tcggaggcga gattgagccg cagggcctcg
      781 tcccaggaga gcgcgcgcaa acgcttgagg gtccccacca gggccccgga cagcgggatc
      841 gcgtcggccc ccggccggca cgccgcgcag acgaggccgc ccgcggcgac gtcgagagcc
      901 gggtcgtcga aggggtggag ccgaccgcag gacatgcagc ggtcgatccg gggccggtga
      961 cccagcaggt ccaccgcgcg gagcccgaag cagaccgcga cccgcgcggg ccgggccgcc
     1021 gtctccatgg cctggagcgc ccgcaccagc agcccgaaca gggcgggctg cggatcgcgg
     1081 tcggcggaga gacgcgccac cacctccacc gcccacgccc cctgccccag ccgctcgagg
     1141 tgctcccgca ccccgacgaa cgagcgcacg atgtcgaagt ggtccacctg caccagctcg
     1201 ctgcgcccgc tgtcgaagaa caccatctcg ccgagggtga acagctcgag cgcgctcccg
     1261 aagcgcgagc gcgggcgccg ggccgagcgg gcgatgcccc gcaccttgcc gtggtcgcgg
     1321 gtgtagaagt cgacgagccg gtcgctctcc ccgaaggcgc gccggccgat gaccaccccg
     1381 cgggtcttgt agagcgccat caggaggtca ggaggaagcc gaactcccgg agggcccggt
     1441 cgtccttgcg ccactccgcg cgcacgcgca ccgtgaggtc gaggaagacc ttgatgccga
     1501 agaagcgctc caggtcggtg cgggcggtgg cgccgatgcg cttgagcatc gccgcgccct
     1561 tgccgatcac gatgccccgc tgcgactcgc gctccacgaa gatgaccgcg cggatgtaga
     1621 ggcactcggg ccggcggcgc tcggtcagct cctccacccg gaccgcgcag gcgtacggca
     1681 cctcctggtg ggtcaggcgg aagatcttct cccggatcgc ctccgcgacc cagaaggtct
     1741 ccggctggtc ggtgagcgcg tcggggggga agtaggccgg ccgctcgggc agggtcgcca
     1801 cgatcagctc gagcagccgc gcgcagtgct cgccgcgctc cgccgagatc ggcacgatct
     1861 ccgcgaacgg atgggcgacc cggtaggccg cgatcagcgg gagcaggcgc gccttcgggc
     1921 tcacgaggtc cgtcttgttg aggcagcaga agacgggggc gcgcgagacc ttgaggcggt
     1981 cgagcgcctc ccggtcgagg cggccggggt cctcggtggc ttccgccacg aagcacacca
     2041 ggtccacgtc ctcgaccgcc cgctctgccg tcttcgacat cagctgcccg aggcggcccg
     2101 cgcccggatg cagacccggc gtgtccacga agatgacctg ggcggacggc aggtggcgga
     2161 tcccggtgat gcgcgtccgc gtggtctgcg ggcgcggcga gacgatcgcc atcttctcgc
     2221 cgaccaggcg gttgagcagc gtcgacttgc ctacgttggg gcggccgacg agggccacga
     2281 aacccgcgcg gtgaggggcc ggcatcaggc ctccaggagc ccggcccaga gccgcgcggg
     2341 caccgcgcgc gtcgagcggc cggccaggat ctcgcgcgcc cgctcgtgca tgcgccgggc
     2401 gcgccgcggg tcgtggtcgt cgtagccggc caggtgcagc aggccgtgca ccaccaagag
     2461 gtcgagctcc accgccagcg gcacccggag ccgccgggcg tggcgcgccg cggtctcggc
     2521 ggagacgatc acctcgcccc agagccgacc ggggccgggc cccgcgaggt cgaaggcgag
     2581 cacgtcggtg gcgcgccgcg tcccgaggta ccgcgcgttg agagcgcgaa tgcgccggtc
     2641 gtcgaccacc gtgacgtgca cctcccggtc cgcccggccg agggccgcga gggcgcgccg
     2701 ggcgaccgcc cgcaggcggc cggggaagac gcgagagcgg cgctggaggt tggagatcgc
     2761 gacgggcacg gccgggccga acctcagccc gctccgcgcg tgggaggctt ccggacgcgg
     2821 tcgtaggccc gcacgatcga cgacaccaga tcgtgccgga cgacgtcccg atcgtcgaag
     2881 tagatgaacc ggatgccgtc gatgccctgc agcacgctct ggatctcgat caaccccgag
     2941 gtgcggctcg cgggcaggtc cacctgggtg atgtccccgg tcacgaccat cttggaattg
     3001 aagccgagcc gggtcaggaa catcttcatc tgctcggagg tggtgttctg ggcctcgtcg
     3061 aggatgatga aggcgtcgtt gagcgtgcgc ccccgcatgt aggcgagcgg cgcgatctcg
     3121 atcgccccct tctcggagag cgagcccacc ttctccggct ccagcatgtc gtagagcgcg
     3181 tcgtagagcg gccggagata ggggtgcacc ttctccacca ggtcgccggg caggaagccg
     3241 agccgctcgc ccgcctccac cgcgggccgg gtgaggatga tgcgcgccac ttcacgcttg
     3301 agcagggccg agacggccat ggccaccgcc aggtacgact tgccggtgcc ggcggggccg
     3361 atcgcgatga ccaggtcgtg gcctcgcacc gcctccaggt accgccgctg attggcggtc
     3421 ttgggggtga cgaaccgccg gcgcgacggc accgcgatcg agtcggcgag caccgacttg
     3481 acgtcgccgc ccggctcgtt gctcgcgatc cgcaaggccg agcggatgtc ggccgcgccc
     3541 agggacggcc gctcccgcag catcgccacc agctcgcgca ggacgcgctc cgcctcgacc
     3601 acctggcgct cttcgccccg cagggtgatg tcgtggccgc gcgcggtgat gcggacgtcg
     3661 tgctgggtct ccagcgtgcg caggtgctcg tcttggcggc cgaggagggc gagatgattg
     3721 aggtccggag gcaggacaat ccgccgggtg acgccggtgg cttcggtcaa cgagcgctca
     3781 tgcctctgcc gagcagcata tcacgcgggt ccgagccctt ccacgacccg gattcccagc
     3841 tcgcggagct gcttggcgtc caccggcgcg ggcgcttcgg tgagcgggca ggtgcccttc
     3901 tgcgtcttgg gaaacgcgat gacctcccgg atggactcct ggcccgccag gttggcggcg
     3961 agccggtcca ggccgaaggc gatgccgccc atgggcggcg ccccgaactc gagcgcatcg
     4021 agcaggaagc cgaatcgcgc gcggccttcc tccttcgaga tgccgatcag gccgaagacc
     4081 cgctcctgca cggtcggctg gtggatgcgg atcgagccgc ccgcggcctc ctgcccgttc
     4141 agcaccaggt cgtaggcctt ggcccgcgcc ttccccggat cggactccag caggggcaga
     4201 tcctcgtcgc gcggcgcggt gaacgggtgg tggaccgcgt cccagcgctt ctcgtccgcg
     4261 ttccactcga cgagcgggaa atcgatgacc cacgcgaaga cgtcgcgatc ggccgggatc
     4321 aggtcgaagc ggcgcgccag gtccacgcgc agtcgcccga gcacggtggc cgccaccatg
     4381 ggggcgtccg cgaccagcag gagcaggtcc ccgggagtgg cgcccagcgt ctcgagcagg
     4441 cggccctgga tactcgtcat gaacttggcc accggagact gcacgccctc cgggctcacc
     4501 ttgatccaca ccaggccctt cgcacccgcc tgcttggcct ccgcggtgag atcgtcgagc
     4561 tccttgcggc tcatgcctcc cgcgcccggc acgcgcagcc ccttgaccgc gccgcccgcg
     4621 gcgaccgtct gggcgaaggc ctggaactcg ctccccgcga acagcgccga gcagtcgacc
     4681 aactcgaggc cgaagcggag gtcgggcttg tcggagccgt aacggccgat cgcctcgtcg
     4741 taggcgatgc gcgggaacgg cagcgccagc tccacgccct tgaccgcccg ccagactgcg
     4801 gccaccagac cctcgatgat cggcaggaaa tcgtcccggt cgaggaagga ggtctcgatg
     4861 tcgatctggg tgaactcggg ctggcggtcc ttgcggagat cctcgtcccg gaagcagcgc
     4921 acgatctgga agtagcgctc gaagcccgcc accatcagca gctgcttgaa gagctggggg
     4981 gactggggga gcgcgtagaa gctgccgggc tgaagccggc tcgggaccag gaagtcgcgc
     5041 gcgccttcgg gggtggagcg ggtgagaaac ggggtctcga cctcgaggaa tgcctcggcg
     5101 tgcaggtagt cgcgggccgt gcggcacacc tggtcgcgga tctgaaacgc gcgcagcatg
     5161 tggggccggc gcaggtcgag atatcggtac ttgagccgca gcgtctcgtc gatgtccgcg
     5221 tcgtcctcca gctggaacgg cagcgggcgg gactcgttga ggatgcggat ctcggtgacc
     5281 tggacctcca ccgcgccggt ggcgagccgg gggttctcgg tcccggcggg ccgggccccc
     5341 acggtccccc gcaccgccag cacgaactcc gcgcgcaccg actccgcctt ggcgtgagca
     5401 ccgccgcccg cgcccgggtt gaagacgcac tgggtgagac cctcgcgatc gcgcaggtcg
     5461 acgaacacga ggcctccgtg gtcgcgccgg cggtacgccc atcccatcag ggtcaccgcc
     5521 tgccccacgt gctcgctccg cagcgccccg cagctgtggg tgcgccgcca cccgcccagg
     5581 ctctccgtca cgctttcgtc tcctccgccc cggccgtccg gggctcgcgc cactcgaccg
     5641 tcccgtccgc gcccaccacc ccgatccggc ggaacccgcg cgcttccaga tcttgcagct
     5701 gcttgccgag ccgcttgccg cgggtctcca gcagcacgtg acggccctgc tcccgcaggt
     5761 cgcgggcgag gcccagcacc ggggtcaagt ccatcgtatc gtcgaacacc agcgccaccc
     5821 gaag
//
LOCUS       DATMCV010000151         4953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_874219, whole genome shotgun sequence.
ACCESSION   DATMCV010000151 DATMCV010000000
VERSION     DATMCV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874219"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VNU03_03630"
     CDS             <1..884
                     /locus_tag="VNU03_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWP73571.1"
                     /translation="MHACGHDGHTAVGITLAEILAARRAELPGTAVFLFQPAEEVLGG
                     ARPMIEAGALENPHVEEIYGLHFTTQQRVGQVQVRSGPSMASADAFTVEVRGTGGHGA
                     MPHLSIDPITAAANILLGMQSLVSREIPAQETAVLTVGQIVAGTKGNILPDRAVMRGT
                     IRAFDAGVRDHLITRLGAFVSHVAQAYRAEAEMRLDGGSCPAVVNPAPESAFVRECAS
                     RELGDEAVTEGRVVMASDDMSLFLRERPGCYFRVGIGSASGAARPHHAPEFEMNEAGL
                     PVGLQVGLAVMRTALGR"
     gene            899..1666
                     /locus_tag="VNU03_03635"
     CDS             899..1666
                     /locus_tag="VNU03_03635"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP73572.1"
                     /translation="MGRLDGKVAAVTGGASGIGEATARRFVAEGAAVALGDIDAERGR
                     QVAGEIRGAGGRAVFVETHAGRETDATRFVRTAREEFGRLDVLVNNAGMRLYQTVVEA
                     SEESWDAILAVNVKGYAFCAKAAIPLMREGGGGSIVNVASIRSIVAGGNMVQYDTTKA
                     AVAGLTRAMAQDHAAEGIRVNAVCPGPIFTRFHERRAAAAGRTAEDFRTEFGRGTMLG
                     RAGTPAEVAACILFLASDDASYVTGASLYVDGGHTAR"
     gene            complement(1696..4191)
                     /locus_tag="VNU03_03640"
     CDS             complement(1696..4191)
                     /locus_tag="VNU03_03640"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWP73573.1"
                     /translation="MSLLRRLGPLVMLTLALAPPAAAATKDCRHSTPLPDDVRLRAPA
                     PDVPADVARFAGAWSGVWLDPGGEEVLCQTLIVEEVFPNGYARIVHSRGSHPGWNLSH
                     PTSWRATGRVADGLLRFTLPTPDRVELTYRFAGDALASTVSDRQGSHGTLTRLDELSQ
                     VECGRTERRAPVTPPAATRDRLTAAELLAPSLPAQGPVHNDYFRPIGTSGAAKHTLAG
                     TLTIGTAPISASSQGCPSLPTPSQAVTLEFFTAGEHLVPVVRDIAPPPRRLIVSPGRV
                     WSEPGDGGMSRASFPFIVVNPVDNGAHNGVATFLFDDTHVSGLRVQVVQETSPWERLD
                     FWGHLPLTYVPGPIANEAALRADFAEEVRRQMPMQPWAALPATPALAAFDGDPAPTDI
                     SANGLVVDGVLYVRGCNTRYGPFPYCREMRHGVFSVTKSLGAAVALLRLAQKYGDAVL
                     DEKLVDYLPPGSQPHPGWDGVTFADALDMATGIGDEGRERDGSTSADENGAKMLRWIV
                     KRPLREKLEIALSYGKYPWDRGEIFRYNTTQTFVLAVALDTYLKRREGPDAHLWDMVR
                     TEVFRPIGILHAPAMHTIEGDGGRGVPLLGYGLYPTVDDVAKLTTLLQNGGRHEGVQL
                     LSATKLAEALYRTDPPSGLPSGLRNRFGAGRYHLSFWSVPYKTSTGCFFQIPEMIGYG
                     GNLVALLPNHVSVFRFADGFDFDVEAMIQAGESLGPFCVPVAAPEPTPPRVALTESEL
                     ATEIVGHVLAVGPQRLFFAAGGRLYGASGKEVDVGNWEISSEGRLCRRWHVWDHGRAR
                     CYVVYRKDDGFELEVPERFTRFVARPHGGAFDE"
     gene            complement(4269..4742)
                     /locus_tag="VNU03_03645"
     CDS             complement(4269..4742)
                     /locus_tag="VNU03_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73574.1"
                     /translation="MGTRPLAPRATAWVGTLVALVGFVILFVALGIIPIDESNFHAPH
                     WVVGAAGLAFFLAGLAILTGPPASTPEASRTTWRSFVLGLGVVGALAAVFNWVAFGPG
                     PRSFSGGISIPFVSVFGPQSEWSGRVAFGLAAGLIDLFLVWVIVRGLRDLLGRDR"
     gene            complement(4743..>4953)
                     /locus_tag="VNU03_03650"
     CDS             complement(4743..>4953)
                     /locus_tag="VNU03_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005245291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP73575.1"
                     /translation="PEHIADAALFLASEDSRFITGQVLVVDGGLTAMGPDLFGHEAGS
                     RLLRKAGLNTGSTGLPGAVREVQSP"
BASE COUNT          723 a         1719 c         1864 g          647 t
ORIGIN      
        1 tgatgcacgc ctgcgggcac gacggccaca ccgcggtggg catcacgctg gccgagatcc
       61 tggccgctcg ccgcgccgag ctgcccggca ccgcggtctt cctgttccag cccgcggagg
      121 aggtgctggg cggcgccagg ccgatgatcg aggccggcgc gctcgagaac ccgcacgtcg
      181 aggagatcta cgggctccac ttcacgaccc agcagcgggt cgggcaggtc caggtgcgtt
      241 ccgggccgtc gatggcctcg gccgacgcgt tcacggtgga ggtgcgcggc accggcgggc
      301 acggcgcgat gccgcatctc tcgatcgacc cgatcaccgc ggcggccaac atcctgctcg
      361 gcatgcagag cctcgtgtcc cgcgagatcc cggcccagga gaccgcggtg ctcacggtgg
      421 gacagatcgt cgccggcacc aagggcaata tcctgccgga ccgggcggtc atgcgcggga
      481 ccatccgcgc gttcgacgcg ggggtgcgcg atcatctgat cacgcggctc ggggccttcg
      541 tctcccacgt cgcgcaggcg taccgggccg aggcggagat gcgcctggac ggcggctcct
      601 gcccggcggt ggtcaacccg gcgcccgaga gcgcgttcgt gcgggagtgc gcgagccgcg
      661 agctgggcga cgaggcggtc accgaggggc gcgtggtgat ggcgagcgac gacatgagcc
      721 tcttcctgcg cgagcgcccc ggctgctact tccgggtcgg catcgggtcg gcgagcggcg
      781 cggcccgccc gcaccacgcg cccgagttcg agatgaacga ggccgggctg ccggtcggcc
      841 tgcaggtggg gctggccgtg atgcgcaccg cgctcgggcg ctgaccggcg cgtcggcgat
      901 gggacgcctc gacggcaagg tggcggcggt gacgggtggt gcgtccggga tcggcgaggc
      961 caccgctcgc cgcttcgtgg ccgagggcgc ggcggtcgcg ctgggcgaca tcgacgcgga
     1021 gcggggccgc caggtggcgg gggagatccg gggtgcgggc ggccgcgcgg tgttcgtgga
     1081 gacgcacgcc ggccgcgaga ccgacgcgac gcgcttcgtg cggaccgcgc gcgaggaatt
     1141 cgggcggctc gacgtgctgg tgaacaacgc gggcatgcgg ctctaccaga cggtggtcga
     1201 ggcgagcgag gagagctggg acgcgatcct cgcggtcaac gtcaagggct acgcgttctg
     1261 cgcgaaggcg gccatcccgc tgatgcgcga gggcgggggc ggcagcatcg tgaacgtcgc
     1321 gtcgatccgc tcgatcgtcg cgggaggcaa catggtgcag tacgacacca ccaaggccgc
     1381 ggtggccggc ctcacccgcg cgatggcgca ggaccacgcg gccgagggca tccgggtcaa
     1441 cgcggtgtgc cccggtccga tcttcactcg tttccacgag cgccgggccg ccgcggcggg
     1501 gcggaccgcg gaggacttcc ggaccgagtt cgggcgcggc actatgctcg ggcgggcggg
     1561 caccccggcc gaggtcgcgg cctgcatcct cttcctcgcc tccgacgacg cctcctacgt
     1621 caccggcgcc tcgctctacg tcgacggcgg gcacaccgcg cgctgaccca aacggcggcc
     1681 gggtcttccg gcccctcact cgtcgaaggc gcccccatgg ggccgcgcga cgaaccgggt
     1741 gaaccgttcc gggacctcca gctcgaagcc atcgtccttg cgatacacga cgtagcagcg
     1801 ggcgcggccg tggtcccaga cgtgccagcg gcggcagagc cggccctccg acgagatttc
     1861 ccagttcccc acgtccacct cctttcccga cgcgccgtag agccggcctc ccgcggcgaa
     1921 gaagaggcgc tggggcccga ccgcgaggac gtgcccgacg atctcggtgg ccagctcgct
     1981 ctcggtcaag gccacgcgcg ggggcgtcgg ctccggcgcg gccacgggaa cgcagaaggg
     2041 cccgagggac tcgccggcct ggatcatggc ttcgacatcg aaatcgaagc cgtcggcgaa
     2101 tcggaagacg gagacgtgat tcggcagcag ggcgacgagg ttgccgccgt atccgatcat
     2161 ctcggggatc tggaagaagc agccggtgct ggttttatag ggcacggacc agaaggacag
     2221 gtgatagcgc ccggcgccga atcgatttcg caggcccgac gggagtccgc tcggcgggtc
     2281 ggtccggtac agcgcctcgg ccagcttcgt cgcgctcagc agctggacgc cctcgtgccg
     2341 gcccccgttc tggagcagcg tggtcagctt cgcgacgtcg tccacggtcg ggtagaggcc
     2401 gtagcccagc aggggcaccc cgcggccgcc gtcgccctcg atcgtgtgca tcgccggggc
     2461 gtgaaggatc ccgatcggac ggaacacctc ggtccgcacc atgtcccaga ggtgagcgtc
     2521 ggggccctcc cggcgcttga gatacgtgtc cagcgcgact gctagcacga acgtctgggt
     2581 ggtgttgtag cggaagatct cgccgcggtc ccaggggtac ttgccgtagg agagcgcgat
     2641 ctcgagcttc tcgcggagcg gacgcttcac gatccagcgg agcatcttcg ctccgttctc
     2701 gtcggcggac gtgctcccgt cgcgctcccg gccctcgtcc ccgatgccgg tggccatgtc
     2761 gagcgcgtcc gcgaaggtga cgccgtccca gcccgggtgc ggctggctgc ccgggggcag
     2821 gtagtcgacc agcttctcgt cgaggaccgc gtcgccgtac ttctgggcca gccgcagcag
     2881 cgcaaccgcg gcgcccagtg atttcgtcac cgagaagacc ccgtgccgca tctcgcggca
     2941 gtaggggaac gggccatagc gggtgttgca gccccgcacg tagagcacgc cgtcgaccac
     3001 gaggccgttg gcgctgatgt cggtcggagc ggggtcgccg tcgaaggccg cgagggcggg
     3061 ggtggccggc agggcggccc agggttgcat gggcatctgg cgccgaacct cttccgcgaa
     3121 gtcggcgcgc agcgcggcct cgttcgcgat cggccccggg acgtaggtca gcggtaggtg
     3181 gccccagaaa tcgagccgct cccacggcga cgtctcctgc acgacctgga cgcggagccc
     3241 ggatacgtgc gtatcgtcga agagaaacgt ggccacccca ttgtgcgccc cgttgtcgac
     3301 cgggttcacc acgatgaacg ggaacgaggc gcgggacatg ccgccgtcgc cgggctcgga
     3361 ccacacccgt cccggcgaga cgatcagcct gcggggcggc ggcgcgatat cgcggacgac
     3421 cgggacgaga tgctcgccgg cggtgaagaa ctcgagcgtc acggcctggg atggcgtggg
     3481 caggctcggg cagccctggc tcgaggcgga gatcggcgcg gtcccgatcg tgagggtgcc
     3541 cgcgagcgtg tgcttcgcgg cgcccgacgt cccgatcggc cggaagtagt cgttgtggac
     3601 cgggccctga gccgggagcg agggcgcgag cagctccgcg gcggtcaatc ggtcgcgcgt
     3661 ggcggcgggc ggcgtcaccg gagcccggcg ctccgtccgc ccacactcga cctgtgacag
     3721 ctcgtcgaga cgggtcagcg tgccgtggct gccctgccga tcactgacgg tcgaggcgag
     3781 cgcgtcgcca gcgaagcggt acgtcagctc cacccggtcc ggggtcggca gcgtgaaccg
     3841 gagcagccca tcggccacgc ggccggtcgc gcgccacgag gtcgggtgcg acaggttcca
     3901 gcccgggtgc gagccgcggc tgtggacgat ccgggcgtac ccgttgggga agacctcctc
     3961 gacgatgagc gtctggcaca gcacctcctc gcctcccggg tccagccaca ctccgctcca
     4021 ggcgcccgcg aagcgagcca cgtccgccgg cacgtccgga gccggcgctc tcagacgcac
     4081 atcgtcgggc agcggggtcg agtgccggca gtccttggtc gcggccgcgg ccggtggggc
     4141 gagcgccagc gtgagcatca ccagcggccc gagtcgtcgg agcagagaca tcggcgtcct
     4201 cccgtgggtc gggatgggtc ggacagagga tagcgcgagt tgcagcaggt gacgagacgg
     4261 tcgcgcgtct agcggtcgcg accgagcagg tcgcgcaggc cccggacgat cacccatacg
     4321 aggaacaggt cgatgagccc ggccgcgagg ccgaaggcga cccggcccga ccattcactc
     4381 tgcggcccga agaccgacac gaacgggatc gagatcccgc cgctgaagga ccgcggcccg
     4441 gggccgaacg ccacccagtt gaagaccgcg gcgagcgccc ccacgacccc gaggccgagc
     4501 acgaagctcc gccaggtggt gcgactcgcc tccggcgtgc tcgccggcgg cccggtgagg
     4561 atcgcgaggc cggccaggaa gaacgcgagg ccggccgcgc cgaccaccca gtgcggtgcg
     4621 tggaagttcg actcgtcgat cgggatgatg cccaaggcca cgaagaggat cacgaagccc
     4681 acgagcgcga cgagcgtgcc gacccacgcg gtcgcccggg gtgcgagcgg gcgcgtcccc
     4741 acctaggggc tctgcacctc gcgcaccgcg ccgggcagcc cggtcgaccc ggtgttgagc
     4801 ccggccttcc ggagcaggcg gctccccgcc tcgtgcccga agagatccgg ccccatcgcg
     4861 gtgaggccgc cgtccaccac caggacctgg ccggtgatga agcgcgagtc ctcgctggcc
     4921 aggaagaggg cggcgtcggc gatgtgctcg ggc
//
LOCUS       DATMCV010000152         3519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_408946, whole genome shotgun sequence.
ACCESSION   DATMCV010000152 DATMCV010000000
VERSION     DATMCV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408946"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1666
                     /gene="zwf"
                     /locus_tag="VNU03_03655"
     CDS             158..1666
                     /gene="zwf"
                     /locus_tag="VNU03_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWP73576.1"
                     /translation="MTASTIDESCTLVIFGASGDLTRRKLLPALWSMFQSRVLPEPFA
                     VVGVSRSEMTNEQFRARMREAITDFARVQPPSTRVWERFAQALFYYAGDPADGGMYAG
                     LSAYLKQVEQERGTGANRLFYVSTPPSVYSPLVTRLGEAGLNRPAENTNGWVRIIIEK
                     PFGRDLASAGALNQVVASVFSEDQVYRIDHYLGKETVQNILVFRWANGIFEPLWNRNH
                     VDHVQITVGEAIGVEGRGAYYEESGALRDMIQNHILQLLCLVAMEPPVTFDADPVRDE
                     KTKVMRAIRPIAADEVDRVAVRGQYGPGFVGGQRVSGYREEKGVSAESITETFAALRL
                     EIENWRWAGVPFYLRTGKRLPKRASEIAVQFKQTPHLVFRRNPEILTEPNRLVLRIQP
                     DEGMSLSFGAKLPGPELRIKPVEMEFDYGQAFGGEPPEAYERLLLDAMKGDATLYARG
                     DWVMMAWELLQPVLSGWGAGDPRKFPNYEAGTWGPAEADTLIERSGRRWRRP"
     gene            complement(1695..2102)
                     /locus_tag="VNU03_03660"
     CDS             complement(1695..2102)
                     /locus_tag="VNU03_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73577.1"
                     /translation="MRRFLVLSVTALALTACASTPPKVQRSEFEDIPVPKGLAVDLNR
                     STIIESPAVKAARLFYKGRIEPDSLSVAFRTTLEANGWKHLSSTTASDKGTTQVYEKA
                     GSSLQVLIYESWYYTWVEMSATRMVGQAAAALR"
     gene            2178..2822
                     /locus_tag="VNU03_03665"
     CDS             2178..2822
                     /locus_tag="VNU03_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWP73578.1"
                     /translation="MRRRPLLTALLVVLALLALVVVAHRPLLRAAGHALVVEEPGPPA
                     DAIVVVAGGTPSREARAAALFQAGGAPRVLVSRQFVPGRVQTLIEMGIRPLDFQGESV
                     RVLEKLGVPRDAIVTLDRSVEITETELRHVVAAAKARGWHRVTLVTSGLHARRVRLIW
                     RREAAGAIEAGIAVAPDECTSEDGWWRRRRCSEAVLHEYLGLLALYLNVSSWMR"
     gene            complement(2842..3195)
                     /locus_tag="VNU03_03670"
     CDS             complement(2842..3195)
                     /locus_tag="VNU03_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73579.1"
                     /translation="MPINSRYLFSAAMDVDPAQERLFNEVYDREHVPSLLTVPGVVAV
                     SRYELQPLTMSIGGQLKTIVIENAPKYGALYEIESPDVLISEAWAKAVEQGRWPAQVR
                     PHTRNRQHTLYRRLP"
BASE COUNT          472 a         1211 c         1257 g          579 t
ORIGIN      
        1 ttctccacgt tgaaggccgc ccaggcgctg ggcgatctcc agagtctgcg cgacgccgcg
       61 cgccgggtca tccggctcca tctgaacggc aagcagtcgc agggcctgca gcagctgctg
      121 cagatggtcc agggactggc caagaggtta tgacgtcatg accgcctcca cgatcgacga
      181 atcctgcacc ctggtgatct tcggcgcctc gggtgacctc acccggcgca agctcctgcc
      241 cgcgctctgg tccatgttcc agagccgcgt gctccccgag ccgttcgcgg tggtaggggt
      301 gtcccgctcc gagatgacca acgagcagtt ccgcgcccgc atgcgcgagg ccatcaccga
      361 cttcgcgcgc gtgcagccgc cctccacgcg ggtgtgggag cgcttcgccc aggctctctt
      421 ctattatgcg ggcgatccgg ccgacggcgg gatgtacgcc ggcctcagcg cgtacctcaa
      481 gcaggtcgag caggagcgcg gcaccggcgc caaccggctc ttctacgtct ccaccccgcc
      541 ctcggtgtac tcgccgctgg tgacgcgtct cggggaggcc ggcctcaacc ggcccgccga
      601 gaacaccaac gggtgggtgc gcatcatcat cgagaagccg ttcgggcgcg acctcgcctc
      661 ggcgggcgcg ctcaaccagg tggtggcgag cgtcttcagc gaggaccagg tctaccggat
      721 cgatcactat ctcggcaagg agacggtcca gaacatcctg gtgttccggt gggccaacgg
      781 catcttcgag ccgctctgga accgcaacca cgtcgaccac gtgcagatca cggtgggcga
      841 ggccatcggg gtcgagggcc gcggcgccta ctacgaggaa tcgggcgcgc tgcgggacat
      901 gatccagaac cacatcctcc agctcctctg cctggtggcg atggagccgc cggtcacctt
      961 cgacgccgac ccggtgcgcg acgagaagac caaggtgatg cgcgcgatcc gccccatcgc
     1021 ggcggacgag gtggaccgcg tcgcggtgcg cggacagtac gggccgggct tcgtgggagg
     1081 ccagcgggtg tcgggctacc gcgaggagaa gggcgtgtcg gccgagtcga tcaccgagac
     1141 cttcgcggcg ctgcggctcg agatcgagaa ctggcggtgg gcgggggtgc cgttctacct
     1201 gcgcaccggc aagcgcctgc ccaagcgggc gagcgagatc gcggtgcagt tcaagcagac
     1261 cccgcacctc gtcttccgtc ggaacccgga gatcctcacc gagcccaacc ggctcgtgct
     1321 ccggatccag cccgacgagg gcatgtcgct ctcgttcggc gcgaagctgc cgggcccgga
     1381 gctgcgcatc aagccggtcg agatggagtt cgactacggg caggccttcg gcggcgagcc
     1441 gcccgaggcg tacgagcgcc tgctgctgga cgccatgaag ggcgacgcca cgctctacgc
     1501 gcgcggcgac tgggtgatga tggcgtggga gctgctgcag ccggtcctct ccggctgggg
     1561 cgccggcgat ccgcggaaat tccccaacta cgaggccggc acctggggtc cggccgaggc
     1621 cgacacgctg atcgagcgca gcgggcggcg ctggcggcgc ccctaggggc gccgccgtga
     1681 cgcgacgctc gtcgctatct gagcgcggcc gccgcctggc cgaccatccg cgtggccgac
     1741 atctccaccc aggtgtagta ccagctctcg tagatcagca cctggagcga gctgcccgcc
     1801 ttctcgtaga cctgcgtggt gcccttgtcg gatgcggtcg tcgacgacag gtgcttccac
     1861 ccgttcgcct cgagcgtggt gcggaacgcg accgacaagc tgtccggctc gatgcggccc
     1921 ttgtagaaga gccgcgccgc cttcaccgcc ggcgactcga tgatcgtgga tcggttcagg
     1981 tccaccgcga gccccttggg caccgggatg tcctcgaact cgctgcgctg gaccttgggc
     2041 ggtgtcgagg cgcacgcggt cagcgcgagc gccgtcaccg aaaggacgag aaaccgtcgc
     2101 atgggtagta cctcctggcg tcgatcgtac ttcccatggg gctccacacc aaagtttttg
     2161 gcgcggcaaa tgtgcgcatg agacgccggc cgctcctgac cgccctgctc gtggtgctgg
     2221 cgctgctcgc gctggtcgtg gtggcgcacc gtccactgct tcgcgcggcg ggccacgccc
     2281 tcgtggtgga ggagcccggc cccccggccg acgcgatcgt ggtggtggcg ggtggcaccc
     2341 ccagccggga ggcgcgggcg gccgcgctct tccaggccgg cggggcgccg cgggtcctgg
     2401 tctcgcgcca gttcgtcccc gggcgcgtcc agaccctgat cgagatgggt atccgcccgc
     2461 tcgacttcca gggcgaatcg gtgcgggtgc tcgagaagct cggcgtgccc cgggatgcga
     2521 tcgtcaccct ggaccgctcg gtggagatca ccgagaccga gctccgccat gtggtcgccg
     2581 cggccaaggc ccgcggctgg caccgggtca cgctggtcac cagcggcctc cacgcccgcc
     2641 gcgtccggct catctggcgc cgggaggcgg cgggcgcgat cgaggcgggc atcgcggtgg
     2701 cgccggacga gtgcacgagc gaggacggct ggtggcgccg ccgtcgctgc agcgaggcgg
     2761 tgctgcacga gtacctcggg ctcctcgctc tctacctcaa cgtgtccagc tggatgcgct
     2821 gaggcggccg ccggctcccg ctcaggggag gcggcggtag agggtgtgct ggcggttgcg
     2881 cgtgtgcggc cgcacctgcg ccggccaccg cccctgctcc accgccttcg cccacgcctc
     2941 gctgatcagc acgtcggggc tctcgatctc gtagagcgcg ccgtactttg gcgcgttctc
     3001 gatgacgatc gtcttgagct gccccccgat actcatggtg aggggctgca gctcgtagcg
     3061 ggagaccgcc accacgccgg ggacggtcag caggctcggc acgtgctcgc ggtcgtagac
     3121 ctcgttgaag aggcgctcct gggcagggtc gacgtccatg gcggcgctga agaggtagcg
     3181 ggagttgatg ggcatggcgt ggctccttgt ctggggggtg ctgggttggt ggttgttcgg
     3241 gttggcggtt gttcgggtcg gggctaccgg ttgtgggctg ctgccgttag cttctttatt
     3301 tatccgctcg gctcgccttc ggctgcgcct cgccaagcca ctgcacttat ccgctcggct
     3361 cgccttcggc tgcgcctcgc caagccgctg cgggtccgcg gacgggcgac cggggctggg
     3421 cggacactcg cggcggtcta ccgtttcctg ctcttgctgc cgctcctggc ctttggcttg
     3481 gcggtcgtgg ccccggcgcg gccgtagtcg tcctcgcgg
//
LOCUS       DATMCV010000153         3418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_408947, whole genome shotgun sequence.
ACCESSION   DATMCV010000153 DATMCV010000000
VERSION     DATMCV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408947"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VNU03_03675"
     CDS             complement(<1..729)
                     /locus_tag="VNU03_03675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWP73580.1"
                     /translation="MSDDFRAVAVDAARRAGAFLRSRFGTRPTISYKGSPTNLVTEMD
                     RGAETIIVEAIRARFPDHAILAEESGALPGAPAHRWIVDPLDGTTNYAHGLPIYAVSI
                     GLEVAGELAVGVVYDPSREECFVAERGRGATLNDAPIRVSTTPRMAESLLGTSYPNDI
                     RGAPRDNLAEHRALIFRCHSVRSIGSAVLGLAAVAAGRLDGYWEQRLGAWDVAAGALL
                     IREAGGTVTSVYGGPLDLGAPSITA"
     gene            792..1724
                     /locus_tag="VNU03_03680"
     CDS             792..1724
                     /locus_tag="VNU03_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWP73581.1"
                     /translation="MRIVVMGSGGTGGYFGAKLARAGEDVTFVARGAHRAAIAASGLQ
                     IRSAIEGEWTVRAPAVERLDGRPPADLVLFCVKSFDTEEAAALIGPVVGPRTGVLSIQ
                     NGVDNEEKLGRVLGAGHVLGGAVRVFATIEAPGVIRHTFGGHMTFGEMDGRETERARA
                     FLAAFRKAEIPTELVPDVERALWDKYVFLTTHAGMTALTRCPAGVLRALPEVRELYRR
                     IVGEIVAVGRAAGAPVDDGLLDQGMKFLDAVAPNAYSSLHHDLIHGKRLELEALHGHV
                     VRLAERHRVAVPTVFAIYAALRPYRDGAPAAPAG"
     gene            1776..>3418
                     /locus_tag="VNU03_03685"
     CDS             1776..>3418
                     /locus_tag="VNU03_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73582.1"
                     /translation="MSSFKRLLPFLLRYRLRYLGGAACLLVSTTMAVGIPWTVKRAVD
                     ALERDGAAAHLGPFVLLILLLAAAHGIARLGSRFAMLGAGQWVEHDLRTALYARLLTL
                     PPAFYHLHRTGDLMSRASNDISALRALAGFGSVMLIGTLFAFVGTLGAMWVIDPWLTL
                     FAMAPFPGLVVIAKRFNHDVEVRSTAVQEQLGALSAKAQENLTGMAVVRAYTMEEREI
                     AEFARLNAEYRTRNIALARIQSVSWPLMGLVSGVGALIVLWLGGKAVVDGRISLGSFV
                     AFNGYLAQLAWPTIALGWTLASIQRGLAAMQRVTEILDARPHESLGGLSGEPGLSGAW
                     GSAGAISGPPQMKKNAPDQPPTFLPGGPTPELPPDGPQPELLPNGALEFKHLTFAYDD
                     RGPALRDVSFVVPAGSVVAVVGPTGSGKSTLGTLLCRLYEPPRGAVWIGGCDVLDLPL
                     GPLRRSVGYVPQESFLFSRPLRDNVRLADEHASPERLEEVARTAGLADDLGALPAGWD
                     TVVGERGLTLSGGQRQRVALARALVADPPFLVLDDVLASV"
BASE COUNT          414 a         1226 c         1271 g          507 t
ORIGIN      
        1 ggcggtgatg gacggggcac ccaggtcgag cggcccgccg tacaccgacg tgacggtgcc
       61 gcccgcctcg cggatcagga gcgcgcccgc ggccacgtcc caggccccga gccgctgctc
      121 ccagtagccg tcgagccggc cggcggccac cgcggccagc ccgagcaccg cggagccgat
      181 ggagcgcacg ctgtggcagc ggaagatgag cgcgcggtgc tcggcgaggt tgtcgcgcgg
      241 cgccccacgg atgtcgttgg ggtagctggt gccgaggagg ctctcggcca tgcggggcgt
      301 ggtggagacg cggatcggcg cgtcgttgag ggtggcgccg cgcccgcgct cggccacgaa
      361 gcactcctcg cggctcggat cgtagaccac gcccaccgcc agctcgcccg cgacctcgag
      421 gccgatcgac accgcgtaga tgggcaggcc gtgcgcgtag ttggtggtgc cgtcgagcgg
      481 gtccacgatc cagcggtgcg cgggggcgcc gggcagcgcg ccgctctcct cggccaggat
      541 cgcgtggtcc gggaagcgcg cgcggatcgc ctccacgatg atcgtctccg cgccgcggtc
      601 catctcggtg accaggttgg tggggctgcc cttgtagctg atcgtgggcc gggtcccgaa
      661 gcgggagcgg agaaaggcgc cggcccgtcg ggccgcgtcg accgccaccg cgcgaaagtc
      721 atcgctcacg aactccgaga atagcacggc gctgcttgac aacccggccg gcctctcgca
      781 gcatgacgcg catgcggatc gtggtgatgg gctcgggcgg caccggcgga tacttcggcg
      841 cgaagctcgc gcgggcgggc gaggacgtga ccttcgtggc gcgcggagcg caccgggctg
      901 cgatcgccgc gagcggcctg cagatccgat ccgcgatcga aggggagtgg acggtccgag
      961 ctcccgcggt cgagcggctc gacggccgcc cgcccgcgga cctggtgctc ttctgcgtga
     1021 agtccttcga caccgaggag gccgcggccc tgatcgggcc ggtggtcggg ccccggaccg
     1081 gggtgctctc gatccagaac ggggtggaca acgaggagaa gctcggccgc gtgctgggcg
     1141 cgggccacgt cctgggcggc gcggtgcggg tcttcgcgac catcgaggcg cccggcgtca
     1201 tccgccacac cttcggcggg cacatgacct tcggggagat ggacgggcgg gagaccgagc
     1261 gcgcgcgcgc ctttctcgcc gccttccgca aggcggagat ccccaccgag ctggtgccgg
     1321 acgtcgagcg ggcgctctgg gacaagtacg tgttcctcac cacccacgcg ggcatgaccg
     1381 cgctcacccg ctgcccggcc ggggtgctgc gggcgctgcc cgaggtgcgc gagctgtacc
     1441 ggcggatcgt cggcgagatc gtcgcggtcg ggcgcgcggc cggagccccg gtggacgacg
     1501 gtctgctcga tcagggcatg aagttcctcg acgcggtggc gccgaacgcc tactcctcgc
     1561 tgcaccacga cctgatccac ggcaagcggc tcgagctgga ggccctgcac ggccacgtgg
     1621 tgcggctggc cgagcgccac cgggtggcgg tgcccaccgt gttcgcgatc tacgccgcgc
     1681 tccgtccgta tcgcgacggc gcgcccgccg ctcccgccgg ctagccgcgg ccccgccggg
     1741 cggtcgcgcg cccggcgctg ttctatactg acccggtgtc ctcgttcaaa cgtctcctgc
     1801 cgtttctgct ccgctatcgc ctgcgctacc tcggcggggc cgcgtgtctc ctcgtctcga
     1861 cgaccatggc ggtcggcatc ccgtggacgg tgaagcgcgc ggtggacgcc ctcgagcgcg
     1921 acggggccgc cgcccacctc ggccccttcg tgctcctcat cctgctcctg gccgcggcgc
     1981 atgggatcgc ccggctcggg tcgcgcttcg cgatgctggg cgcggggcag tgggtcgagc
     2041 acgacctccg caccgcgctc tacgcgcgat tgctgaccct gccgcccgcg ttctaccatc
     2101 tccatcgcac cggcgacctg atgtcacgcg cctccaacga catctcggcc ctgcgggcgc
     2161 tggccggctt cggcagcgtg atgctgatcg gcaccctgtt cgcgttcgtg ggcaccctcg
     2221 gcgccatgtg ggtgatcgac ccgtggctca ccctgttcgc gatggccccg ttcccgggcc
     2281 tggtggtgat cgccaagcgc ttcaaccacg acgtggaggt gcgctccacc gcggtccagg
     2341 agcagctggg cgcgctctcg gccaaggccc aggagaacct caccggcatg gcggtggtgc
     2401 gcgcgtacac catggaggag cgggagatcg ccgagttcgc gcggctcaac gcggagtacc
     2461 ggacgcgcaa catcgccctg gcacggatcc agtcggtgtc gtggccgctg atggggctcg
     2521 tgtccggcgt gggcgccctg atcgtcctct ggctgggcgg caaggcggtg gtggacggcc
     2581 gcatctccct cggctcgttc gtggccttca acggctacct ggcccagctc gcctggccca
     2641 ccatcgccct cggctggacc ctggccagca tccagcgcgg gttggcggcg atgcagcggg
     2701 tgacggagat tctcgatgcg cgcccgcatg agagcttggg cggcctatcc ggcgagccag
     2761 ggttgtccgg ggcttggggg agtgcggggg ccatttctgg gcccccgcag atgaagaaaa
     2821 acgcgccaga tcaaccgcca accttcctgc ccggcggacc gacgcccgag ctcccgccgg
     2881 atggtccgca acccgagctc ctgcccaacg gcgcgctcga gttcaagcac ctcacgttcg
     2941 cgtacgacga tcgcgggccc gcgctgcggg acgtgtcgtt cgtggtgccg gcgggctcgg
     3001 tggtggcggt ggtggggccc accgggagcg ggaagtcgac cctcggcacg ctgctctgcc
     3061 ggctctacga gccgccgcgc ggcgcggtgt ggatcggcgg ttgtgacgtg ctcgacctgc
     3121 cgctcgggcc gctgcgccgc tcggtgggat acgtgccgca ggagtccttc ctcttctccc
     3181 gtccgctccg cgacaacgtg cggctggccg acgagcacgc ctcgcccgag cggctcgagg
     3241 aggtggcccg caccgcgggc ctggccgacg atctgggcgc gctgcccgcg ggctgggaca
     3301 cggtggtcgg ggagcgcggc ctcaccctgt ccggcggcca gcgccagcgg gtggcgctgg
     3361 cgcgcgcgct ggtcgcggac ccgccgttcc tggtcctcga cgacgtgctc gcatcggt
//
LOCUS       DATMCV010000154         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_874315, whole genome shotgun sequence.
ACCESSION   DATMCV010000154 DATMCV010000000
VERSION     DATMCV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874315"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VNU03_03690"
     CDS             complement(<1..207)
                     /locus_tag="VNU03_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73583.1"
                     /translation="MPRMEPLGIHEVDDEIRHMCEESERQIGTSASTRTYARNPAVVK
                     ALATFRAALAREGTIDPVIRELVRI"
     gene            complement(278..523)
                     /locus_tag="VNU03_03695"
     CDS             complement(278..523)
                     /locus_tag="VNU03_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWP73584.1"
                     /translation="MARVVLIGNLAQLTGGVAEFQLSATSVKQLFQQLAELHPALGPH
                     LQEGVAVAIDGQIYQDTLFEPIGPDSEVFVLPQIAGG"
     gene            complement(534..>2003)
                     /locus_tag="VNU03_03700"
     CDS             complement(534..>2003)
                     /locus_tag="VNU03_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP73585.1"
                     /translation="YLEPLAIALSRKAKRPVKMVMSREEVFKASGPTSGATVRVKLGA
                     TKDGKFVAAFAELKYQAGAFQGSPVQPGTMCAYAPYDIEHVHVIGYDVVSNRPKVAAY
                     RAPGAPISEYAVESVVDEIAKKLRLDPIELRMMNAAHEGTKAAYGPKFGPIGLEETLN
                     SIKKTEHWNTPLRKWQGRGVASGFWFNIGGETSVSLTVNEDGTISLMAGTPDIGGLRA
                     ALASVVADELGVPYDKVRPIIGDTGSVSFNFLTGGSRSAFSGSMAIVEAAKQIKNELL
                     ARACKLWEAKPDQVEFKDGAVRPLGALAEKVKPMTIADFAKISGKLGGPIGGVGRINA
                     QGAGPSFGTHLVDLEVDPETGRTTILRYTVAQDAGKALHPAYVEGQFQGGAVQGIGWA
                     LNEEYIYGDDGKLQNAGFLDYRMPVASDVPMIDAILVEVGNPKHPYGVRGVGETPIVP
                     PLGAIANAMENATGIRFTELPMSPPKVLKALQEQGRNKA"
BASE COUNT          270 a          700 c          688 g          345 t
ORIGIN      
        1 gatccgcacc agctcgcgga tgaccgggtc gatggtcccc tcgcgggcca gggcggcccg
       61 gaaggtggcg agcgccttga ccaccgccgg gttgcgggcg taggtgcggg tgctggccga
      121 ggtgccgatc tgacgctcgg actcctcgca catgtgccgg atctcgtcgt cgacctcgtg
      181 gatcccgagc ggttccatcc gtggcattga cgcctccatc tctccctctc ccctccgggg
      241 agagggcagg gtgaggggtg gtcagggtga ggggtggtta tccgcccgcg atctgcggga
      301 ggacgaagac ctcgctgtcg gggccgatcg gctcgaacag cgtgtcctgg tagatctgcc
      361 cgtcgatggc caccgcgacc ccttcctgca ggtgcgggcc gagggcgggg tgcagctcgg
      421 cgagctgctg gaagagctgc ttcacggacg tcgctgatag ctggaactcg gcgacgcccc
      481 cggtgagctg cgcgaggttg ccgatcagca ccactcgcgc catgcgtcgg ctctcaggcc
      541 ttgttgcggc cctgctcctg cagcgccttc agcaccttgg gcggcgacat cggcagctcg
      601 gtgaaccgga tgccggtggc gttctccatg gcgttggcga tggccccgag cggcggcacg
      661 atcggcgtct ccccgacgcc gcgcacgccg tacgggtgct tcggattgcc gacctcgacc
      721 aggatcgcgt cgatcatcgg tacgtccgag gccaccggca tgcggtagtc gaggaagccc
      781 gcgttctgga gcttgccgtc gtcgccgtag atgtattcct cgttcagcgc ccagccgatg
      841 ccctgcaccg ccccgccctg gaactgcccc tccacgtacg ccgggtgcag cgccttgccc
      901 gcgtcctgcg ccacggtgta gcgcaggatc gtggtccgcc cggtctcggg atccacctcg
      961 agatccacca ggtgggtgcc gaagctcggg cccgcgccct gggcgttgat gcgcccgacg
     1021 ccgccgatgg gcccgcccag cttgccggag atcttcgcga aatccgcgat ggtcatcggc
     1081 ttgaccttct cggcgagcgc gccgagcggc cgcaccgcgc cgtccttgaa ctcgacctgg
     1141 tccggcttgg cctcccacag cttgcaggcc cgcgcgagga gctcgttctt gatctgcttg
     1201 gccgcttcca cgatggccat cgagcccgag aaggcggagc ggctgccgcc ggtgaggaag
     1261 ttgaagctga ccgacccggt gtcgccgatg atcggccgca ccttgtcgta cgggacgccc
     1321 agctcgtcgg caaccaccga ggcgagcgcc gcgcgcaggc cgccgatgtc cggggtaccg
     1381 gccatcagcg agatggtgcc gtcctcgttc acggtgagcg agaccgaggt ctcgccgccg
     1441 atgttgaacc agaagccgga ggccaccccg cggccctgcc acttccgcag cggggtgttc
     1501 cagtgctcgg tcttcttgat cgagttgagc gtctcctcga gaccgatggg cccgaacttc
     1561 ggcccgtagg ccgccttcgt cccctcgtgg gccgcgttca tcatgcggag ctcgatcggg
     1621 tcgaggcgca gtttcttggc gatctcgtcc accacgctct cgaccgcgta ctccgagatc
     1681 ggcgcgccgg gagcgcggta ggccgccacc ttcgggcggt tcgacaccac gtcgtagccg
     1741 atcacgtgaa cgtgctcgat gtcgtagggc gcgtacgcgc acatggtgcc gggctgcacc
     1801 ggagagccct ggaaggcgcc cgcctggtac ttcagctccg cgaaggcggc cacgaacttg
     1861 ccgtccttgg tggcgccgag cttgacccgg accgtcgcgc ccgacgtcgg cccggaggcc
     1921 ttgaacacct cctcgcgcga catcaccatc ttgaccggcc gcttggcctt ccgggagagc
     1981 gcgatcgcga gcggctcgag gta
//
LOCUS       DATMCV010000155         2203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_671645, whole genome shotgun sequence.
ACCESSION   DATMCV010000155 DATMCV010000000
VERSION     DATMCV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671645"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VNU03_03705"
     CDS             complement(<1..232)
                     /locus_tag="VNU03_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HWP73586.1"
                     /translation="MAATPDAGPISSARKWAITLSVMVVAFMQVLDTSVTNVVLPHLQ
                     GSLSAGLEEVSWVITSYLAANAIIIPATGWLAG"
     gene            complement(249..1373)
                     /locus_tag="VNU03_03710"
     CDS             complement(249..1373)
                     /locus_tag="VNU03_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWP73587.1"
                     /translation="MVISAKVAGHVASIHVDDNRAVKAGDLLLRIDPRDYAAKRDQAR
                     AAVAVAAASQQATRSETQLTRETTAAQVEEARAALESARVAEQAAQSAVVETRATLEA
                     KHAATAAMTAEVTGTQSTQRQTAREKERMRRLLEGGFVAQRDFDQAEMTAGTAAATLE
                     ATTRRLNQAEKEAQQAEAALTSRILAVNQARQRINEARAALGRVESQRRQVIVKEAEI
                     ERAVAALSQAQADLAFAELQLAHAEVRAPGDGVVSKKSVEVGQVVQMGQPLLALVPLQ
                     DVWVVANFKETQLSRVRPGMPATVEIDTFPGQVFHGTVDSLSAGTGSRFSLLPPENAT
                     GNWVKVVQRVPVKIRLDPREFGNPHTLRAGMSAYVVIRVR"
     gene            complement(1541..>2203)
                     /locus_tag="VNU03_03715"
     CDS             complement(1541..>2203)
                     /locus_tag="VNU03_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73588.1"
                     /translation="EMQGPRAEAVAVGITMPVGPPQFAQAGSLAGPYLLPPDAFAWVS
                     EVVARRGGYSAQTRIVEVAPTPPLTQNGRTAYLLADQMNHGRPYRSFALVNSAALGNG
                     YWQYYMTLLTAPAEVFPQALPQMTEIWQSWSISQGEMNRRTAQAMLTMQETNRIMQST
                     AEGRRTTEWHQRLTGMTLQGRWVVEDTTTGQRQELSSQEINSLFERFPGRYRVVPSSD
                     LK"
BASE COUNT          277 a          812 c          778 g          336 t
ORIGIN      
        1 ccccggcgag ccagccggtg gcgggaatga tgatggcgtt ggccgcgagg taggaggtga
       61 tgacccacga cacctcctcg agcccggcgg agagcgatcc ctgcagatgc ggcagcacca
      121 cgttggtcac gctggtgtcg agcacctgca tgaaggccac caccatcacc gacagggtga
      181 tggcccactt gcgcgcggag gagatcgggc cggcgtccgg ggtcgcggcc accgcgacgc
      241 tcgctccgct accgcacccg gatgacgacg tacgccgaca tcccggcccg cagggtgtgg
      301 ggattgccga actcgcgcgg atccaggcgg atcttcaccg gcacgcgctg gactaccttg
      361 acccagttcc cggtcgcgtt ctcgggcggc agcagcgaga agcgcgagcc ggtgcccgcg
      421 ctgagcgaat cgacggttcc gtggaagacc tggccgggga acgtgtcgat ctccacggtg
      481 gccggcatcc ccggccgcac ccgggagagc tgggtctcct tgaagttggc gaccacccac
      541 acgtcctgca gcggcaccag cgcgagcagg ggctggccca tctgcaccac ctgccccacc
      601 tccacgctct tcttcgacac caccccgtcg ccgggcgccc gcacctccgc gtgcgcgagc
      661 tgcagctccg cgaacgcgag gtcggcctgc gcctggctga gcgcggcgac cgcgcgctcg
      721 atctcggcct ccttcacgat gacctggcgc cgctggctct ccactcgccc gagcgcggcg
      781 cgcgcctcgt tgatgcgctg ccgggcctgg ttcaccgcga ggatccggct ggtcagcgcg
      841 gcctcggcct gctgcgcctc cttctcggcc tggttcaggc gccgcgtggt cgcctcgagc
      901 gtggccgccg cggtgccggc cgtcatctcg gcctggtcga agtcccgctg ggcgacgaag
      961 ccaccctcga ggagccggcg catccgctcc ttctcccgcg cggtctggcg ctgcgtgctc
     1021 tgcgtcccgg tcacctccgc ggtcatggcc gcggtggccg cgtgcttggc ctcgagggtg
     1081 gcccgcgtct cgaccaccgc cgactgggcc gcctgctccg cgacccgggc cgactcgagc
     1141 gcggcgcgcg cctcctcgac ctgggccgcg gtggtctcgc gcgtcagctg ggtctcggag
     1201 cgggtggcct gctggctggc cgccgcgacc gccaccgcgg cccgggcctg gtcgcgcttg
     1261 gccgcgtagt cgcgcggatc gatccggagc aggaggtcgc cggccttgac cgcgcggtta
     1321 tcgtcgacgt gaatcgaggc cacgtggccc gcgaccttgg cgctgatgac cacgacgttg
     1381 ccctcgacgt acgcgtcgtc ggtggcaacg tgcttgcggc tctcccacca cgtagccgcc
     1441 gtagatcagc gccaccagcg agaccgctgc ggtcaggccg atcagacgcc ctcgtctcat
     1501 ggcccgggta tgctaccacc ggtgcccgat gaggcgccgg ctacttgagg tcggacgacg
     1561 gcaccacccg gtagcggccc gggaaccgct cgaagaggct gttgatctcc tgcgaggaga
     1621 gctcctggcg ctggccggtg gtggtgtcct ccaccaccca gcgcccctgg agcgtcatgc
     1681 cggtcagccg ctgatgccac tcggtggtgc gccgcccctc ggcggtcgac tgcatgatgc
     1741 ggttggtctc ctgcatcgtc agcatcgcct gggcggtgcg gcgattcatc tcgccctggc
     1801 tgatcgacca cgactgccag atctcggtca tctggggcag cgcctgcggg aagacctcgg
     1861 ccggcgcggt gagcagcgtc atgtagtact gccagtagcc gttgccgagc gcggcgctgt
     1921 tcaccagcgc gaacgaccgg tacggccgcc cgtggttcat ctggtccgcg agcaggtagg
     1981 cggtgcggcc gttctgggtg agcggcgggg tgggcgccac ctccacgatg cgcgtctgcg
     2041 cggagtagcc gccgcggcgg gcgaccacct ccgacaccca cgcgaacgcg tcgggcggca
     2101 gcaggtacgg cccggccagg gagccggcct gggcgaactg ggggggcccg accggcatcg
     2161 tgatcccgac cgcgacggct tcggcccgcg gcccctgcat ctc
//
LOCUS       DATMCV010000156        11277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_874362, whole genome shotgun sequence.
ACCESSION   DATMCV010000156 DATMCV010000000
VERSION     DATMCV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874362"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3832)
                     /locus_tag="VNU03_03720"
     CDS             complement(<1..3832)
                     /locus_tag="VNU03_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWP73589.1"
                     /translation="MSPSGNWKLLGRSPAIRSIRDDLERVLPRLAPGRRVPPILLQGE
                     TGTGKGLLARTIHEASPRAAGPFVDLNCAAIPATLIEAELFGSERGAFTDARQSRTGL
                     VQASHTGTLFLDEIGLLPEGLQAKLLTVLESREVRPLGGTRARPVDVLIIAASNSDLR
                     EAVRERRFREDLYHRLAVLVFSMPPLRERGEDVLELADAFLARACADHALPPKSLGDD
                     ARSAIARYAWPGNVRELANLMDRMALLTEGEVIAAADLDLPAGPEPDAPLKASVDRFT
                     RERMEWALRDSHGNISAAADRLGVARSTFRYQLERLGLAPDRDGRSKPRAEMPPRPLA
                     ERILAPVGTVAAERKQVTVLFADLKELLERMAGHDPEATRKLVDPILERMIEVVRRHG
                     GTVNQVRGDGIMALFGAPLALEDHAVRACYAALAMHEAVALPGGAAQVRVGVHSGDVV
                     LRSIGGEARLDYGAVPETTPVAAQMEQAAVPGTSRISGATRQLAEGFVEVAATGLAHV
                     FELRAPSAARSRLAASITRGLTVFVGRDAETAQIRAALDCARDRRGQLVTLVGEPGVG
                     KSRLTWEVTRAARSEGWLVLQTGSTSTSTAAPYLPLADLLREYFEIEPGTEPARIRER
                     VAGKLQALDEGLGPLAVPLLAILDVPANDADWERREGRERRRRMLDGARLLILSASRG
                     APVLVVVDDLQWIDSETHAFLDLLVESLPAARVMLLVNYRPEGGHGWSGKSYYTQIRV
                     DPLAAPGAGELLDALVGPEPALDGLKRLLVERTAGNPFFLEESVWALVETRALVGERG
                     AYRLLTPLVNVEVPATVQAVLAARIDRLPPEGKRLLHAASAIGMDVPLALLQSIADLP
                     DESVRRGIEHLLAAEFLYETGLWPNLEYTFKHALTHDVAYGSLLPEHRRTLHARIVEA
                     IERLHAGRLDEHIDRLGHHALRGEVWDRAVDFLGRAAEAVTRVAYADAAAYYERALGA
                     LARLPEDLARREQAVDLQFELARCLYSTGHFDRATAAYREAERLAAALGDDDRVARVC
                     TGLAYLLGSEADHHGSIEAGRRALALAIKVGDLELQTWTDVGLAREYFAIGDYRRGIE
                     RARAALDTSTPATVRFRNLPTPVGSRTWLALCLASTGQFVEAVTWAEAALALAERAES
                     VLAQVWASYTLARVHCIRGHFAPALTLLERAAALLERGTFPIYAPRVLASLGAVYTVG
                     GRAKDGLALLDRAVAEGEASRILYDHAMVLTQLGEAHLECGPEESERRATQALELARG
                     HGERANEAWVMHLLG"
     gene            4014..4232
                     /locus_tag="VNU03_03725"
     CDS             4014..4232
                     /locus_tag="VNU03_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73590.1"
                     /translation="MTTPIRIAIGVYLLVVGVLAGMTIDHMRYDVQRSEVLGRYEEAL
                     HEWQAYRMALEKEEAAPKASASWSASAN"
     gene            complement(4259..5455)
                     /locus_tag="VNU03_03730"
     CDS             complement(4259..5455)
                     /locus_tag="VNU03_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWP73591.1"
                     /translation="MASNGIRDRVAIVGMGCTSFGERWDKSVSDLLVESSQDALNSAN
                     VPIDAVDAFWLGTLFSGQSGLTLSRPLKLDYKPVSRLENYCATGSEAFRNACYAVACG
                     AYDIAMAIGVEKLKDSGISGLPSIGAPEDGTRAGLTAPATFSLLAPAYAKKYGVDEAT
                     FKEAMTRIAWKNHKNGALNPRAQFRKEVAKDVIACSPQVAGALGIFDCSGVSDGSAAA
                     IIVRAEDAYKYTDKPLFVKALSFIAGPATGPIDPDYDYTTFPEVVHSAADAYAQAGIK
                     DPRAELAMAEVHDCFTPTELVLMEDLGFAQRGTAWKEVLNGTFDLEGELAVNPDGGLK
                     SFGHPIGASGLRMLFEAWLQLRGEAGKRQIASIARGRKLALTHNLGGAPGECVSFVSV
                     VGSERS"
     gene            complement(5478..5981)
                     /locus_tag="VNU03_03735"
     CDS             complement(5478..5981)
                     /locus_tag="VNU03_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73592.1"
                     /translation="MASAAVNELKKKSDAAAANLWKQLQGMEPNLDKSDAAGEWTTRQ
                     VLCHLLGDPEVKPAAVLQSFTVGSLPVIDIKPGYAPVTPERQTMTLAQLREALETQRR
                     GLLSYLDSLENGDLGRKAKIPLFKQFMGTDEIALPVYVGAMFDYHWNDHAGQIAKIRK
                     ANGMPDA"
     gene            complement(5954..8404)
                     /locus_tag="VNU03_03740"
     CDS             complement(5954..8404)
                     /locus_tag="VNU03_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP73593.1"
                     /translation="MAMVSSAMAGTAAAPDAAGPDAAAFPFRCELSLAPLITFWTQLS
                     AYHEFGRGPIPGLVREKAREAPELAGVIDDLSVIGTHQTFVDLMMTALFPPAFWEQEY
                     GAALFPLELRAFYATPPFRRSLMNEDGTLQGRASFLQKRSVDPAMAAERLWLAYELIL
                     ERVYGLELGGDVPVMMFTTTDSATGLDQHFRLQFDWRFVDVQVVGPKPPLPDGVRQEL
                     AAGRIDSEKLRLMLPPDRFVLRGFMIVKAVDVTDQEVLSSLKRDLIDKDSIVSSSHFL
                     DLQAKLRTFFRRPSLRLGLAAVEGDRVLVLNDASRHEQACIFADSAHHTTAEFTGSLY
                     ERAVVQDSPVVIEDLVAYPDRTPVEEELIQSGVRTFICAPLHYQDRVIGTMELVSDHV
                     GDLNATHLPKLQEVLPLFSMAVQRSVEELNSRIQTVINEQCTAIHPSVEWRFRKAVLN
                     AFERQRGSASVVTELEPIVFEGVYPLFGLADIRGSSTQRGRAIQADLLQQLRLASAVI
                     QSASETRPGPALDELAFRIDSRAAQIERGLNSSDEIGVIAFLRTQVEGLLDHLGTFGA
                     GVRERIAAYRAGLDPRLGTVYHRRRMFEESVTAIAESISSYLEMEQQVAQGMFPHYFE
                     KQKTDGVDYQIYVGAALLENGRVDPLCLKNLRLWQLMITCGMAVRAHQLRDRLPIPLE
                     TTHLILVQHAPLSIRFRFDEKRFDVDGAYDIRYEIMKKRIDKALIQGTAERVTQPGKV
                     ALIYSQPGEAQEYRAYIEYLQGLGYLTGGVEQLDLEELQGVQGLRALRVQVALDSPKL
                     QERINQGADQWPVRQSTS"
     gene            complement(8571..9923)
                     /locus_tag="VNU03_03745"
     CDS             complement(8571..9923)
                     /locus_tag="VNU03_03745"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP73594.1"
                     /translation="MSERVPRVVIVGGGFGGLVAAKALRRAPAEVTLIDRTNHHLFQP
                     LLYQVATSALTPGQIGSPIRGILRGQRNTTVLMGEVTAVNTEQRYVVANSPDRAGVPV
                     AYDYLVLATGVRHSYFGHDEFEPFAPGLKSLGDAVAIRNRILQAFEQAEAEEDPRRHR
                     DLLTFVLVGAGPTGVEMAGALAVLVRTTLRSEFRRIDPLSARIVLVDMASRVLGSFDE
                     ALSTAAKRRLERLGVEVRLGRAVDHIDEDGITVAGDRIASKTVIWTAGVAPSPAGKWL
                     GVETDRAGRVKVQPDLTVPGHPEVFVVGDTASLQQDGKPLPGVAQVAMQQGRYAGRLI
                     QQRISGTPTSRPFRYFDKGNMAIVGKGFAVLQSGRIRVSGFLAWLAWAAVHLEFLAQS
                     SLRVSVFVQWVWTYLTGQRGSRLIVAHRASPGPGFLAKKPETTEAHAQPMGASGGTVS
                     "
     gene            complement(9940..10899)
                     /locus_tag="VNU03_03750"
     CDS             complement(9940..10899)
                     /locus_tag="VNU03_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWP73595.1"
                     /translation="MSYDKSIGRIAIVGTGVIGASWAALYLARGLSVVATDPAPNAEA
                     NLRRYIDAAWKDLTVIGLSPNASRDRLEFTLDMKKALDGADFVQENGPERQDFKIKLF
                     AEMDAATPADSIIASSSSGLTMSVMQSACRRPDRCVIGHPFNPPHVVPLVEVVAGEKT
                     SPRVVQQAIAFYASIGKKPIHVRKEVVGHVANRLQAALYREVVHLIEQGVLDVADSDA
                     AVCWGPGLRWGVMGPNLLFHLGGGQGGIRHFMEHLSGPVATWWKDLGKLTEFSPQIKQ
                     TIIDGVLNEADNRSIEELERERDSMLLELLATRAKVEKARGVA"
     gene            complement(10902..>11277)
                     /locus_tag="VNU03_03755"
     CDS             complement(10902..>11277)
                     /locus_tag="VNU03_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HWP73596.1"
                     /translation="KGQTPANRKDIEVLFDGLPEPIDLDLDLSKRMMYWTDRGDPPRG
                     NTVNRAPIDGPRPDAPEIVLTHLMEGIGLALDVAGDRMFVTDFGGSVYSARLDGSEKK
                     MFLYAQGNLTGVAYAEVPVKEK"
BASE COUNT         1743 a         3969 c         3815 g         1750 t
ORIGIN      
        1 ggccgagcag gtgcatcacc cacgcctcgt tcgccctctc cccgtggccc cgcgccagct
       61 cgagggcctg agtggcccgt cgctcggatt cctccggtcc gcactccagg tgagcctcgc
      121 cgagctgggt gagcaccatg gcgtgatcgt agagaatacg gctcgcttcg ccctcggcga
      181 cggcgcggtc cagcagagcg aggccgtcct tcgctcggcc gccgaccgtg tagactgcac
      241 cgagggacgc gagcactcgc ggcgcgtaga tggggaacgt gccccgctcc agcagcgcgg
      301 cggcccgctc cagcaacgtg agcgccggcg cgaagtggcc ccggatgcag tgaacgcgag
      361 cgagagtgta gctcgcccat acctgggcca ggacgctctc ggcccgctcc gcgagcgcca
      421 gggccgcctc ggcccacgtc accgcctcga cgaactgccc ggtcgaggcg aggcacaggg
      481 cgagccacgt gcgagagccc accggggtgg gtaggttcct gaaccggacg gtggcaggtg
      541 tcgaggtgtc gagtgccgcc cgggcccgct cgatgccccg ccgatagtct ccgatcgcga
      601 agtactcgcg ggccaggccc acgtcggtcc acgtctgcag ctcgaggtcg ccgaccttta
      661 tggccagggc cagcgcgcgc cggccggcct cgatcgaccc gtgatggtcg gcctcggacc
      721 cgagcaggta cgccagcccg gtgcagaccc gcgccacgcg atcgtcgtcc ccgagggccg
      781 cggccagccg ctcggcctcg cgataggcgg cggtggcgcg atcgaagtgc ccggtggagt
      841 agagacaccg cgccagctcg aactgcagat ccacggcctg ctcccggcgc gcgaggtctt
      901 ccggaagccg cgccagggcg cccagcgccc gctcgtagta ggccgccgcg tcggcgtacg
      961 cgacccgcgt caccgcctcg gccgcgcggc cgagaaagtc caccgcgcgg tcccagacct
     1021 cgccccgcag cgcgtgatgg cccagccggt cgatgtgctc atcgagccgc cccgcgtgga
     1081 ggcgctcgat ggcctccacg atgcgcgcgt gcagcgtccg gcgatgctcg ggcagcaggc
     1141 tgccgtacgc cacgtcgtgg gtgagcgcgt gcttgaaggt gtactcgagg ttcggccaga
     1201 gcccggtctc gtagaggaac tcggcggcca gaaggtgctc gatcccgcgg cgcaccgact
     1261 cgtccgggag gtcggcgatc gactggagca gcgcgagcgg cacgtccatg ccgatggcgg
     1321 acgcagcgtg gagaagtcgc ttcccctccg ggggcaggcg gtcgatccgc gcggcgagga
     1381 cggcctgaac cgtggcgggc acctcgacgt tcacgagggg cgtgagcagg cgataggccc
     1441 cgcgctcgcc gacgagcgcg cgggtctcga ccagcgccca cacgctctcc tcgaggaaga
     1501 acgggttccc cgcggtgcgc tcgaccagca gccgcttcag cccgtcgagc gcgggctcgg
     1561 gccccacgag cgcgtccagc agctcgccgg ctcccggcgc cgccagtgga tcgacccgga
     1621 tctgcgtgta gtagctcttg ccgctccagc cgtgcccgcc ctcgggccga tagttcacga
     1681 ggagcatcac gcgcgcggcg ggaaggctct cgacgagcag gtcgaggaac gcgtgcgtct
     1741 cggagtcgat ccactgcaga tcgtcgacga ccaccagcac cggcgccccg cggctcgccg
     1801 agaggatcag caggcgggcc ccgtcgagca tgcggcggcg gcgctcccgt ccctcacgcc
     1861 gctcccagtc ggcgtcgttc gccggcacgt cgaggatcgc gagcagcggc accgcgagcg
     1921 ggcccagacc ctcgtcgagc gcctggagct tcccggcgac ccgctcgcgg atgcgcgcgg
     1981 gctcggtccc cggctcgatc tcgaagtact cgcggagcag gtcggcgagc ggcaggtacg
     2041 gcgccgcggt gctggtggag gtcgagccgg tctggagcac cagccagccc tcgctccggg
     2101 ccgcgcgcgt gacctcccac gtgagccggg acttgcccac gcccggctcg cccaccagcg
     2161 tcaccagctg gccgcgacgg tcgcgagcgc aatccagcgc cgcgcgaatc tgcgccgtct
     2221 ccgcgtcacg tccgacgaag acggtcagac cgcgcgtgat ggaggcggcc agccgcgagc
     2281 gcgcggcgct cggcgcgcgc agctcgaaga catgggccag cccggtggcc gcgacctcga
     2341 cgaagccctc ggccagctgg cgggtcgcgc cgctgatccg gctggttccg ggcacggccg
     2401 cctgctccat ctgcgccgcc accggggtgg tctcgggaac cgcgccgtag tccagccggg
     2461 cctcgccgcc gatggagcgc agcaccacat cgcccgagtg cacccccacc ctcacctggg
     2521 cggcgccgcc ggggagcgcc accgcctcgt gcatcgcgag cgcggcgtag cacgcgcgca
     2581 ccgcgtggtc ctcgagggcc agcggcgcgc cgaacagcgc catgatcccg tcgccccgga
     2641 cctggttgac ggtgccgccg tgccgacgga ccacctcgat catccgctcg aggatcggat
     2701 ccaccagctt ccgggtggcc tcggggtcgt ggccggccat gcgctccagc agctccttga
     2761 gatcggcgaa gagcaccgtg acctgcttgc gctcggccgc gaccgtcccg accggcgcca
     2821 ggatgcgctc cgcgagcggg cgcggaggca tctccgcgcg cggcttcgag cgaccgtccc
     2881 ggtcgggcgc caggccgagc cgctcgagct gataccggaa cgtcgagcgg gcgaccccga
     2941 gccggtccgc cgccgcggag atgttgccgt ggctgtcccg cagcgcccac tccatccgct
     3001 ctcgggtgaa gcggtcgacc gatgccttca ggggcgcgtc gggctccggc ccggccggca
     3061 ggtcgaggtc ggcggcggcg atgacctcgc cctcggtgag gagcgccatg cgatccatca
     3121 ggttcgccag ctcacggacg ttcccgggcc acgcgtagcg ggcgatggcg gagcgcgcgt
     3181 cgtcgccgag actcttggga ggaagcgcgt gatccgcgca cgcgcgggcg aggaacgcgt
     3241 ccgccagctc gaggacgtcc tcgccgcgct cccgcagggg cggcatggag aagaccagca
     3301 ccgcgaggcg atggtagaga tcctcgcgga accggcgctc gcgcaccgcc tcgcgcagat
     3361 cgctgttcga cgccgcgatg atcaggacgt cgaccggacg ggcccgcgtg ccccccagcg
     3421 gccgcacctc gcgcgactcg aggacggtca ggagcttggc ctgcaggccc tcggggagaa
     3481 ggccgatctc gtccaggaac agcgtcccgg tgtgcgacgc ctgcaccagc ccggtcctcg
     3541 actgccgcgc gtcggtgaac gcgccccgct cggagccgaa gagctcggcc tcgatcaagg
     3601 tcgcggggat ggccgcgcag ttcagatcga cgaaggggcc ggccgcccgg gggctggctt
     3661 cgtggatggt gcgggccagc aggcccttgc cggtgcccgt ctcgccctgc agcaggatcg
     3721 gcggcacccg ccgcccgggc gccagtcgcg gcagcacccg ctccagatcg tcgcgaatcg
     3781 accggatcgc ggggctccgg ccgagcagct tccagttccc gctgggactc acgtcttgac
     3841 tccgtctgaa ggtggcgact tttcgccgtt tcactggcga atcgcccggg cactgaccgg
     3901 ccaggtgatc cgcaactgga cgatatcacg caccctgagc cgtggcacgc ggcctgctcc
     3961 tgtcgcccgc tcagttgact cgaagagccc gatgccaggg caggaggtgc acgatgacta
     4021 ctccaatcag gatcgcaatc ggcgtgtacc tgctcgtggt cggcgtgctg gcgggcatga
     4081 cgatcgacca catgcgctac gacgtgcagc ggtccgaggt cctcggccgc tacgaggagg
     4141 ccctgcacga gtggcaggcg taccggatgg ccctggagaa ggaggaggcg gctccgaaag
     4201 ccagcgcctc ctggtccgcc agcgccaact gatacggcgg ccaactaata cggcggggtc
     4261 agctccgctc ggaaccgacc accgagacga acgagacgca ctcgcccggc gccccgccca
     4321 ggttgtgggt gagcgcgagc ttgcggccgc gcgcgatgct ggcgatctgg cgcttgcccg
     4381 cctcgccgcg cagctgcagc cacgcctcga agagcatgcg cagcccggag gcgccgatcg
     4441 ggtggccgaa ggacttgagg ccgccgtccg ggttcacggc cagctcgccc tcgaggtcga
     4501 aggtgccgtt gagcacctcc ttccacgcgg tgccgcgctg ggcgaagccg aggtcctcca
     4561 tcagcaccag ctcggtgggc gtgaagcagt cgtgcacctc ggccatcgcc agctcggcgc
     4621 gcgggtcctt gatgccggcc tgggcgtagg cgtcggccgc ggagtgcacc acctccggga
     4681 aggtggtgta gtcgtagtcg ggatcgatcg ggccggtggc cggccccgcg atgaacgaga
     4741 gcgccttcac gaagagcggc ttgtcggtgt acttgtaggc gtcctcggcc cgcacgatga
     4801 tcgcggcggc ggagccgtcg gagacgcccg agcagtcgaa gatgccgagc gcgcccgcca
     4861 cctgcggcga gcaggcgatg acgtccttgg ccacttcctt gcggaactgc gcgcgggggt
     4921 tgagcgcgcc gttcttgtgg ttcttccagg cgattcgggt catcgcctcc ttgaaggtgg
     4981 cctcgtccac gccgtacttc ttggcgtagg cgggcgcgag caggctgaag gtggcgggcg
     5041 cggtgaggcc cgcgcgcgtg ccgtcctcgg gcgcgccgat cgacggcagg ccggagatgc
     5101 ccgagtcctt cagcttctcg accccgatgg ccatcgcgat gtcgtaggcg ccgcaggcca
     5161 ccgcgtagca cgcgttgcgg aaggcctcgg agccggtggc gcagtagttc tcgagccggc
     5221 tcaccggctt gtagtcgagc ttcagcgggc gcgagagcgt gagcccgctc tgcccggaga
     5281 agagggtgcc gagccagaac gcgtccaccg cgtcgatcgg cacgttggcc gagttgagag
     5341 cgtcctgtga ggattcgacc agcaggtcgc tcacgctctt gtcccagcgc tcgccgaagc
     5401 tggtgcagcc catgccgacg atcgccacgc ggtcccggat cccgttgctg gccatggtga
     5461 agtctccttt gacccgatca ggcgtcgggc atgccgttgg ccttgcggat cttggcgatc
     5521 tgcccggcgt ggtcgttcca gtggtagtcg aacatcgcgc ccacgtacac cggcagcgcg
     5581 atctcatcgg tccccatgaa ctgcttgaac agcggaatct tcgccttccg tcccagatcc
     5641 ccgttctcga gggagtcgag gtagctcagg aggccgcgcc gctgggtctc gagcgcctct
     5701 cgcagctggg cgagcgtcat cgtctgccgc tccggggtga ccggggcata ccccggcttg
     5761 atgtcgatca ccggcaggct cccgaccgtg aagctctgca aaaccgccgc cggcttgacc
     5821 tccggatccc ccaggagatg acagaggact tgccgggtgg tccactctcc cgccgcgtcc
     5881 gacttgtcca ggttcggctc catcccctgg agctgcttcc agagattcgc cgccgcggcg
     5941 tcgctcttct tcttcagctc gttgactgcc gcactggcca ttggtcggct ccttggttga
     6001 ttcgttcctg aagcttcggg ctgtccaggg cgacctgaac ccggagcgcg cggaggccct
     6061 ggaccccctg cagctcctcc aggtcgagct gctcgacgcc accggtcagg taaccgagac
     6121 cctgcaggta ctcgatgtag gcccggtatt cctgcgcctc gccgggctgg ctgtagatga
     6181 gggccacctt cccgggctgg gtcacgcgct cggccgtccc ctggatgagc gccttgtcga
     6241 ttcgcttctt catgatctcg taccggatgt cgtaggcccc gtcgacgtcg aagcgtttct
     6301 cgtcgaatcg gaaccggatc gacagcggcg cgtgctgcac caggatcagg tgcgtcgtct
     6361 cgagcgggat cgggagacga tcgcgcagct gatgagcgcg gaccgccatg ccgcacgtga
     6421 tcatgagctg ccacagacgc aaattcttca ggcacagggg gtcgacgcga ccgttctcca
     6481 ggagcgccgc cccgacgtag atctgatagt cgactccgtc cgtcttttgc ttctcgaagt
     6541 agtgcgggaa catcccctgc gccacctgtt gctccatctc caggtacgac gagatgctct
     6601 ccgcgatggc ggtgacgctc tcctcgaaca tccggcgccg atggtacacg gtgccgagcc
     6661 gagggtcgag gccggcccgg taggcggcga tccgttcccg gacgccggcg ccgaaggtgc
     6721 cgagatgatc gaggagaccc tcgacctggg tccgcaagaa cgcgatgacc ccgatctcgt
     6781 cgctcgaatt cagcccacgc tcgatctggg ccgcgcggct gtcgatgcgg aacgcgagct
     6841 cgtccagcgc cggtccgggc cgcgtctcgc tcgcgctctg gatcaccgcg ctcgcgaggc
     6901 ggagctgctg gagcagatcg gcctggatgg cccgcccgcg ctgcgtggaa gacccgcgaa
     6961 tgtctgcgag gccgaagagc ggatacacgc cctcgaagac gatcggctcc agctcggtca
     7021 ccacgctcgc gctgccgcgc tgtcgctcga aggcgttgag gaccgccttc cggaagcgcc
     7081 actcgacgct cgggtgaatc gcggtgcatt gctcgttgat gacggtctgg atccggctgt
     7141 tcagctcctc cacgctgcgc tggaccgcca tggagaacag gggcagcact tcctgcagct
     7201 tgggcaggtg ggtcgcattc aggtcgccga cgtgatcgga gaccagctcc atggtaccga
     7261 tcacccggtc ctggtagtgg agcggcgcgc agatgaaggt tcgcaccccg ctctggatca
     7321 gctcctcctc gaccggcgtc cggtccgggt acgccacgag gtcctcgatc acgacggggc
     7381 tatcctggac gaccgctcgc tcgtagagag agcccgtgaa ctcggcggtc gtgtggtggg
     7441 ccgagtccgc gaagatgcag gcctgctcgt gacgcgaggc gtcgttcagg acgagcacgc
     7501 gatctccctc gacggccgcg aggccgaggc ggagactggg ccgccggaag aaggtgcgga
     7561 gcttcgcctg caagtccagg aagtgcgagc tcgacacgat cgagtccttg tcgatcagat
     7621 cgcgcttgag tgaggacagg acctcctggt cggtgacgtc gaccgccttc acgatcatga
     7681 agccgcgcag gacgaatcga tcgggcggca gcatcaggcg gagcttctca ctgtcgatgc
     7741 ggcctgccgc cagctcctgc cgcaccccat cggggagcgg cggcttgggc ccgacgacct
     7801 gcacgtcgac gaatcgccag tcgaactgca gccggaagtg ctgatccagc ccggtggcgg
     7861 aatcggtcgt ggtgaacatc atcaccggga cgtcgccgcc gagctcgagg ccatataccc
     7921 gctcgaggat cagctcgtag gcgagccaga gccgttcggc cgccatcgcc gggtccacgc
     7981 tgcgtttctg caggaagctc gcccgcccct gaagcgttcc gtcctcgttc atgagcgagc
     8041 ggcggaaggg aggcgtcgcg tagaacgcgc gcagctcgag gggaaacagg gccgcgccgt
     8101 actcttgctc ccagaaggcg ggagggaaga gggcggtcat catcaggtcg acgaacgtct
     8161 gatgcgttcc gatgaccgac aggtcgtcga tcacgcccgc cagctccggt gcctcgcgcg
     8221 ccttctcgcg cacgagaccc gggatcggcc cgcggccaaa ctcgtgatag gccgagagct
     8281 gggtccagaa cgtgatcaac ggggcgaggc tcagctcgca ccggaacggg aacgccgccg
     8341 catccgggcc ggccgcgtcg ggagcggcgg ccgttcccgc catcgcgcta ctcaccatcg
     8401 ccatgcaggg attctaccag ccgcgtcgac cgtccggcga tcccgttaag cgctgatcaa
     8461 ttagcgcctt atcgcactgc ggcccagacg gccccgtacc cccgtcgcca ggccggcgcc
     8521 gcgaaccagg agcacaccga tcgccaggat gccgagcagc tcgccgaagg tcacgagact
     8581 gtccctccgc tggcgcccat gggctgcgcg tgggcctccg ttgtctcggg cttcttcgcg
     8641 aggaagccag gtcccggtga tgctcggtgg gccacgatca agcgcgaccc tcgctggccc
     8701 gtcagatacg tccacaccca ttgaacgaag acgctcacgc gaaggctaga ctgggcgaga
     8761 aactcgaggt gcaccgcggc ccacgccagc cacgccagga agccactgac ccgaatccgt
     8821 ccgctctgca ggacggcgaa ccccttcccg actatcgcca tgttcccttt gtcgaagtag
     8881 cggaacggac gcgatgttgg agtccccgag atccgttgct gaatcagtct ccccgcatag
     8941 cgtccctgtt gcatcgcgac ctgagcgacg cccggcagcg gtttcccgtc ctgctggagc
     9001 gaggcggtgt cccccaccac gaacacctct ggatgccccg gcaccgtgag gtcgggctgc
     9061 accttgacgc gtcccgcgcg atcggtctcc acccccaacc acttccccgc cggagacggg
     9121 gccacgccgg cggtccagat caccgtcttg ctcgcgattc gatcacccgc caccgtgatg
     9181 ccgtcttcat cgatgtgatc gaccgcccgg cccagccgca cctccacccc gagccgctcg
     9241 agccgccgct tcgccgccgt cgagagcgcc tcgtcgaacg agcccaatac gcggctcgcc
     9301 atgtctacga ggacgatccg ggcggagagc gggtcgatcc gcctgaactc ggagcggagg
     9361 gtggtcctca ccagcacggc cagcgcgccg gccatctcga ccccggtcgg tccggccccg
     9421 accaggacga aggtcaggag gtcgcggtgc cggcgtggat cctcctcggc ctccgcctgc
     9481 tcgaacgctt ggagaatccg gttgcggatc gccaccgcgt cccccaggct cttcaggccc
     9541 ggcgcaaacg gctcgaattc gtcgtgcccg aagtagctgt gccgaacgcc ggttgccagg
     9601 accaggtagt cgtacgcgac aggcacgccc gcccggtccg gggagttggc gacgacgtag
     9661 cgctgttccg tgttcacggc ggttacctcg cccatgagaa ccgtcgtgtt ccgctgcccg
     9721 cggagtatcc cgcggatcgg agatccgatc tggcccggcg taagggccga ggtcgccacc
     9781 tggtacagca ggggctgaaa caggtgatgg ttggtccggt cgatcaaggt cacctcggcg
     9841 ggcgctcgac gaagagcctt ggcggccacc aggcccccaa aacctccccc gacaatgacg
     9901 actcgcggca cgcgttcaga catagccgct cgtcctcgat cacgccaccc cgcgcgcctt
     9961 ctccaccttc gcgcgcgtgg cgagcaattc cagtagcatg ctgtcgcgct cgcgctcgag
    10021 ctcctcgatc gagcgattgt cggcctcgtt cagcacgccg tcgatgatcg tctgtttgat
    10081 ctgaggactg aattccgtga gcttgccgag gtccttccac cacgtggcta ccgggccgga
    10141 gagatgctcc atgaaatgcc ggattccgcc ttgaccccct ccgagatgga acaggagatt
    10201 gggacccatg actccccaac gcagtcccgg tccccagcac accgcagcat ccgaatcagc
    10261 gacatccagc accccctgct cgatgaggtg cacaacctca cgatagagtg cggcctggag
    10321 acgatttgcg acatgcccca cgacctcttt tcggacatgg atcggcttct tgccgatcga
    10381 tgcgtaaaac gcgattgcct gctgcacgac ccgcggcgag gtcttctcac cggccaccac
    10441 ctcgacgagc ggtaccacgt ggggcggatt gaacggatgg ccgatgacgc agcgatcggg
    10501 acgccggcag gccgactgca tcacgctcat ggtcagcccg gacgagctgg acgcgatgat
    10561 cgaatcagcc ggagtcgcgg cgtccatctc cgcgaacagc ttgatcttga agtcctggcg
    10621 ttcgggaccg ttctcctgca caaagtcggc gccgtcgagg gccttcttca tatcgagcgt
    10681 aaattcgagg cggtcgcgcg acgcgtttgg cgagagcccg atcacggtga ggtccttcca
    10741 agcggcgtca atgtaccgac gcaagttcgc ctccgcattc ggtgctgggt ccgtggccac
    10801 gacgctgagc ccgcgggcga ggtacaaggc cgcccagctc gccccgatga ctccggtgcc
    10861 gacgatggcg attcggccaa tcgacttgtc gtagctcatg atcacttctc cttcacgggc
    10921 acttcggcgt aggccacgcc ggtgaggttg ccctgggcgt agaggaacat cttcttttcc
    10981 gagccatcga gccgggcgga atagaccgag ccgccaaagt cggtcacgaa catccggtcc
    11041 cccgccacat ccagagccag accgatccct tccatcaggt gcgtcagcac gatctcggga
    11101 gcgtctggcc tgggtccgtc gatcggcgcg cgattcacgg tgttgccgcg cggcggatcg
    11161 ccgcgatcgg tccagtacat catgcgcttc gaaagatcga gatcgaggtc gatgggttcg
    11221 ggaaggccat cgaaaagaac ttcgatgtct ttacgattcg ccggcgtttg acccttc
//
LOCUS       DATMCV010000157         3065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_206797, whole genome shotgun sequence.
ACCESSION   DATMCV010000157 DATMCV010000000
VERSION     DATMCV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206797"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..417)
                     /locus_tag="VNU03_03760"
     CDS             complement(55..417)
                     /locus_tag="VNU03_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73597.1"
                     /translation="MAVVLFSHVLFRLRLSATLGVTAPVPLKSPDIYKPLFWGGLWGI
                     PFGLFVRAAWNRLYLAGFLYVLAPLLALFLFFLPISGAGYFGLRHGPAFTIYLLLVNL
                     PFGIVTALAARAIIGLKP"
     gene            complement(510..1211)
                     /locus_tag="VNU03_03765"
     CDS             complement(510..1211)
                     /locus_tag="VNU03_03765"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HWP73598.1"
                     /translation="MRRWARPALILALLVAGVVATRALGLGDLIRLENVARLKQWIEG
                     FGALAPVVFIAGYVLAVVFFVPGLPITVLGGVAFGPVWGTLYVWIAATIGAALAFLVA
                     RYAVRSTVERWVRASPRLARLDDQTADHGWRIVMLTRLVPLFPFNLQNYAYGLTRIGF
                     WPYAITSSICMLPGTAAFTFAGGALSEGRGDVRRTLGYLAVAGVLLVLVSLIPRWLQR
                     RSRVAGDILRNNGGR"
     gene            complement(1208..2716)
                     /locus_tag="VNU03_03770"
     CDS             complement(1208..2716)
                     /locus_tag="VNU03_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HWP73599.1"
                     /translation="MEVLPRDVYNTELIAQVHPPDWRPPTPAPRYNLVVIGAGTAGLV
                     TAAGAAGLGARVALIERHLLGGDCLIVGCVPSKCIIRSSRVVGEVRAAASLGVRVAGA
                     VEPDFGAVMERMRRVRARLSHHDSADRFRGLGVDVFLGDGRFTAPDTVEVNGTRLSFR
                     KAVIATGARAHQPDVPGLAEAGFLTNESVFELTQRPPRLAVIGAGPIGCELAQAFRRL
                     GSEVTVFHTGDHILNREDADAAEIVQRAFVGEGIRLVLGAKLERVERAGGGTVLLATA
                     PSGPVRVEVDQILVGAGRVPNVEGLGLEAVGVAYDRRDGVKVNDRLQTTNPRIYAAGD
                     VCMPEKFTHVADAAARVVIQNALFLGRKTHSALTIPWCTYTDPEIAHVGLYAHDAEKR
                     GMAVDTYLRPFAEVDRAVADGEEDGFVKVHVRRGTDKIVGATIVARHAGEMINELTLA
                     MTAGIGLGRLASVIHPYPTQAEAIRQTGDMYNRTRLTPRVRKLFAAWLRWTR"
     gene            complement(2778..>3065)
                     /locus_tag="VNU03_03775"
     CDS             complement(2778..>3065)
                     /locus_tag="VNU03_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWP73600.1"
                     /translation="KPRVKILPVFALGRLGREGDPRLTEADLAGFDRGLLQCSESRVV
                     GDGGVYACPILAGLPGARLTEETLKESFADAPLYHPACVTCVQTGMTCKNG"
BASE COUNT          519 a         1076 c         1041 g          429 t
ORIGIN      
        1 ccgcgtgctc ggcgaccccg gatcttgtac ggatcgatca ccagccggcg ctggttaggg
       61 cttcagtcct atgatggccc tggcggccag ggcggtgact atcccgaaag gcagattcac
      121 cagtagaaga taaattgtaa acgccggccc gtggcgcaac ccgaagtatc ccgcgccgct
      181 gatcggcaag aagaacaaga acagtgcgag cagcggagcc aggacatata gaaagccggc
      241 gaggtacagc cgattccagg cggcccttac gaacagcccg aaggggatgc cccatagccc
      301 tccccagaag agcggcttgt atatatccgg agacttcaac ggaaccggcg ccgtcacccc
      361 gagcgtcgcg ctgagcctca accgaaacag cacgtggctg aagaggacca ccgccactgc
      421 gcctattacc cccgccgcga agcagatcgc tacccgctgg ccaagggtca aggcactctt
      481 catcgtcttt aaatctcttt ccatcgcatt cacctccctc cattgttcct caggatatcg
      541 ccggcgacac ggcttcgccg ctgcagccag cgcgggatga gcgagacgag cacgagcagg
      601 acccccgcga cggcgagata gccgagcgtg cgcctgacgt cgccccggcc ctcggacagc
      661 gcccctcccg cgaaggtgaa ggccgcggtg ccgggcagca tgcagatgga cgaggtgatg
      721 gcgtagggcc agaagccgat acgggtcagt ccgtaggcgt agttctgcag gttgaacggg
      781 aagagcggca cgagccgcgt gagcatgacg atgcgccagc cgtggtcggc cgtttgatcg
      841 tccaggcgcg ccagccgtgg gctggcccgt acccagcgct ccaccgtgct gcgcaccgcg
      901 tagcgcgcga ccaggaaggc cagcgccgcg ccgatggtgg cggcaatcca gacgtagagt
      961 gtgccccaga cgggaccgaa ggccacaccc ccgagcaccg tgatgggcag cccggggacg
     1021 aagaagacca ccgcgaggac atagccggcg atgaagacga ccggcgcgag ggccccgaac
     1081 ccctcgatcc actgcttgag cctggccacg ttctcgagac ggatcagatc gccgagcccg
     1141 agcgcccgcg ttgccaccac ccccgcgacg agcagggcca ggatcagcgc cggccgcgcc
     1201 caccgcctca tcgcgtccac cggagccatg cggcgaagag cttgcgcacc ctgggagtga
     1261 gccgcgtgcg gttgtacatg tcgcccgtct gccggatggc ctcggcctgg gtcggatacg
     1321 gatggatgac cgaggcgagc cgccccagcc cgatgcccgc ggtcatggcc agcgtcagct
     1381 cgttgatcat ctcgcctgcg tgccgggcca cgatcgtggc gcccacgatc ttgtcggttc
     1441 cgcgcctcac gtgcaccttc acgaagccgt cctcctcgcc gtcggcgacg gcgcggtcga
     1501 cctcggcgaa gggtcgcagg tacgtgtcca ccgccatccc gcgcttctcg gcgtcgtgcg
     1561 cgtagaggcc gacgtgggcg atctcggggt cggtgtacgt gcaccacggg atggtgaggg
     1621 cggagtgcgt cttgcgcccg aggaagagcg cgttctggat gacgacccgg gccgccgcat
     1681 cggccacgtg ggtgaacttc tcgggcatgc agacgtcgcc cgcggcgtag atgcgcggat
     1741 tggtcgtctg gagccggtca ttcaccttga cgccatcccg ccggtcgtag gcaacgccga
     1801 ccgcctcgag gccgaggccc tctacgttgg gcacgcgccc cgcgcccacg aggatctggt
     1861 ccacctcgac cctgaccggt ccgctcggcg ccgtcgcgag caagactgtc ccgccgcccg
     1921 cgcgctcgac ccgctcgagc ttggcgccga gcacgaggcg gatgccttcg cccacgaagg
     1981 cgcgctggac gatctccgcc gcgtccgcgt cttcgcggtt caagatatgg tcgccggtgt
     2041 ggaagacggt cacctcggag cccaggcgtc ggaaggcctg cgccagctcg cagccgatgg
     2101 ggccggcccc gatgacggcg aggcgcggag gccgctgcgt cagctcgaac acgctctcgt
     2161 tggtgagaaa acccgcctcg gcgagaccgg gcacgtcggg ctggtgggct cgcgcgccgg
     2221 tagcgatgac ggccttccgg aacgacagcc gcgtgccgtt gacctcgacg gtgtccggcg
     2281 ccgtgaagcg cccatcaccg aggaagacgt ccacgccgag cccgcggaag cggtcggccg
     2341 agtcgtggtg gctgaggcgc gcgcgcaccc ggcgcatccg ctccatgacg gcgccgaagt
     2401 cgggctccac cgcccccgcc actcgcacgc cgagggaagc ggccgcacgc acctcgccca
     2461 ccacccgcga ggagcggatg atgcacttgg acggcacgca gcccacgatg agacagtcgc
     2521 cgcccagcag gtgccgctcg atcagcgcca cccgcgcgcc caacccggcc gcccccgccg
     2581 cggtgacgag gccggcggtg cccgcaccga tcaccaccag gttgtagcgg ggcgccggcg
     2641 tcggcggccg ccagtcgggc gggtgcacct gagcgatcaa ctcggtgttg tagacgtcgc
     2701 ggggaagaac ttccatcgtt ggcggcatca tggtcctcag gagtgtccgc gcgagtgctc
     2761 cgtggagggc aggtcggcta tccgttcttg cacgtcatgc ccgtctgcac gcacgtcacg
     2821 caggctggat ggtagagggg ggcgtccgcg aaggactcct tgagggtttc ctccgtcagc
     2881 cgcgctcccg gtagccccgc gaggatgggg caggcataga cgccgccgtc tccgaccacg
     2941 cgcgactcgg agcactggag gaggccgcga tcgaagccgg cgagatccgc ttcggtcagc
     3001 cgcggatcgc cctcccgtcc aagacgaccc agggcgaaga cgggcaggat cttcacccgc
     3061 ggctt
//
LOCUS       DATMCV010000158         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_142929, whole genome shotgun sequence.
ACCESSION   DATMCV010000158 DATMCV010000000
VERSION     DATMCV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142929"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VNU03_03780"
     CDS             <1..351
                     /locus_tag="VNU03_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HWP73601.1"
                     /translation="PTGEARITPGFRLPARFVIHAVGPLWQGGAAGEPELLASAYRSS
                     ILLAQEHDLRSVAFPAISTGIFGYPLGPATRVAVETVRAATAKPGSIRQVIFACFGSD
                     VFMAYRDERIPGQV"
     gene            619..>2191
                     /locus_tag="VNU03_03785"
     CDS             619..>2191
                     /locus_tag="VNU03_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HWP73602.1"
                     /translation="MNKIEELKARKDGLDVGADIPRYARLGLESIEEGDLDRLKWWGV
                     FIRKQTPGHFMMRLRIPNGVTTAGQLRAIGGLANRMGRGLVDITTRQQVQLRWIRIQD
                     VPEILDRLLDVGLVTLQTGMDNIRNIIGCPAFGLTPNEVLDASPVARAFTAMFVGNKA
                     YTNLPRKFNVGVTGCRENCTHSETQDIALVPAVKTTGLDEVKGFNVLVGGKNGSGGYR
                     VASSLDVFVRPEEAAEICSAIVLVFRDHGSRDARNKIRLAFLLDEWGEARFREAVEAR
                     VGRRLEKAGADQRLAQSTDHVGVFRQKQPGLNYVGLLVPVGRVTGDQLLELARLSEQY
                     GTGEARLTVDQNVIIPHVRDARLGQMTAEPLLKALRYDPPGVLRGLVSCTGVEFCNLA
                     VIETKSRALEVARALERKVPATKAVRIHWSGCPAGCGNHTVADIGLLGTRTKVDGKVV
                     DAVDIFMGGASGPQASQGVKVLESVPCDELPRVLEGLVRFGEFDRVRRQIRMLQPTSP
                     AAPPPERASPPPVSTI"
BASE COUNT          319 a          767 c          779 g          326 t
ORIGIN      
        1 cccaccggcg aggcgcgcat caccccgggc ttccggctgc cggcccggtt cgtcatccac
       61 gcggtcgggc cgctctggca gggcggcgcg gcgggagagc ccgagctgct ggcctccgcg
      121 taccgctcct cgatcctgct ggcgcaggag cacgatctcc gcagcgtggc gttcccggcc
      181 atcagcaccg ggatcttcgg ctaccccctg ggcccagcga cgcgggtcgc ggtggagacg
      241 gtgcgggccg cgacggccaa gccgggctcc atccggcagg tgatcttcgc ctgcttcggc
      301 tccgacgtgt tcatggccta ccgcgacgag aggattcccg gccaggtctg acccgccccg
      361 ccgcgggccg cctgtttgtt cggcagctcg ctccgcgatg ctcgatgatt tggcagccgc
      421 ggtctcgccc tcacccgagc tggatactgt gaaatacgta gttccggcca tttgtccttg
      481 gccccgcggt tggtacggca gttgcccaca tgatccgaga cacacgaaga tcggcccggc
      541 cctggaggcc gggacttgta ggcgacggcg cctgtcctga acggacggcg ccgtttgtgt
      601 ttcatcgccg gggagcgcat gaacaagatc gaggagctga aggcgcgcaa ggacgggctc
      661 gacgtgggcg cggacattcc gcgctacgcg aggctcggtc tcgagtcgat cgaggagggc
      721 gatctcgacc gactgaagtg gtggggcgtg ttcatccgca agcagacccc gggtcacttc
      781 atgatgcggc tgcgcatccc gaacggggtc acgaccgccg gccagctccg cgccatcggc
      841 gggctcgcca accgcatggg ccgggggctg gtcgacatca ccacgcgcca gcaggtgcag
      901 ctccggtgga tccgcatcca ggacgtcccg gagatcctgg accggctgct cgacgtcgga
      961 ctggtcacgc tgcagaccgg catggacaac atccggaaca tcatcggctg ccccgcgttc
     1021 ggcctgactc cgaacgaggt gctggacgcc tccccggtgg cccgggcctt caccgcgatg
     1081 ttcgtgggga acaaggccta cacgaacctg ccgcggaagt tcaacgtcgg cgtcaccggc
     1141 tgccgcgaga actgcaccca ctccgagacc caggacatcg ccctggtgcc ggcggtgaag
     1201 accaccggcc tcgacgaggt caagggcttc aacgtgctcg tcggcggcaa gaacggctcc
     1261 ggcggctacc gggtggcctc gtcgctcgac gtgttcgtgc ggccggagga ggcggccgag
     1321 atctgcagcg cgatcgtgct ggtgttccgc gaccacggct ctcgcgacgc gcgcaacaag
     1381 atccgcctgg ccttcctgct cgacgagtgg ggcgaggcgc ggttccgcga ggcggtggag
     1441 gcgcgggtcg ggcggcggct cgagaaggcg ggcgccgacc agcgtctcgc ccagtccacc
     1501 gaccacgtcg gggtctttcg ccagaagcag cccgggctga actacgtggg gctgctcgtt
     1561 ccggtggggc gcgtcaccgg cgatcagctc ctcgagctgg cccgcctgtc cgagcagtac
     1621 gggaccggcg aagcgcgcct gacggtggac cagaacgtca tcatccccca cgtgcgcgac
     1681 gccaggctcg gccagatgac cgccgagccg ctactcaagg cgctccgcta cgatccgccc
     1741 ggcgtgctcc ggggcctggt gagctgcacc ggggtcgagt tctgcaacct ggccgtgatc
     1801 gagaccaaga gccgcgccct cgaggtggcc cgcgcgctgg agcgcaaggt gcccgccacc
     1861 aaggcggtgc gcatccactg gtcgggctgc cccgcggggt gcggcaacca cacggtggcg
     1921 gacatcgggc tcctcggcac ccggacgaag gtcgacggca aggtggtcga cgcggtggac
     1981 atcttcatgg gtggtgcctc gggtccgcag gcgagccagg gcgtcaaggt gctcgagagc
     2041 gtgccctgcg acgagctgcc gcgcgtgctg gaggggttgg tccgcttcgg cgagttcgac
     2101 cgcgtgcgcc ggcagatccg catgctccag ccgaccagcc cggccgcccc gccgcccgag
     2161 cgcgcgagcc cgccgccggt ctcgacgatc c
//
LOCUS       DATMCV010000159         2952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_671837, whole genome shotgun sequence.
ACCESSION   DATMCV010000159 DATMCV010000000
VERSION     DATMCV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671837"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VNU03_03790"
     CDS             <1..335
                     /locus_tag="VNU03_03790"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP73603.1"
                     /translation="RRGHRVGPAMPSVKVAKATEVPVGQGLVVQAGAKSLALWNVDGA
                     YHVIDNTCPHRGGPMGDGDLDGRFAICPWHGWRWDVTTGANANNPAVKIACFPARVVD
                     GDVYVELP"
     gene            332..1363
                     /gene="moaA"
                     /locus_tag="VNU03_03795"
     CDS             332..1363
                     /gene="moaA"
                     /locus_tag="VNU03_03795"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWP73604.1"
                     /translation="MIRDTLDRPLRNLRLSVTDRCNLRCQYCMPEEEYVWLPRDDVLT
                     FEEMAQLVDVFTDLGVDKVRLTGGEPLLRRDLDQLVRLLAGNARLRDLALTTNGVLLA
                     EQGAALRAAGLHRVTVSLDTLRADRFRLLTRRDTHARVLEGIASVGQGGWPGLKLDTV
                     VMRGVNDDELADLVEYAGSVGAEVRFIEYMDVGGATHWSMERVVSRREMLGRLSERYG
                     SITPIEPVAEEESAAPADRFRLPDGRVFGIISSTTEPFCRACDRSRLTADGLWYLCLY
                     ATRGVDLRGALRGGASRDELLRLIRSTWQGRRDRGAEERLALPERRPLIQIGELKRDP
                     HLEMHTRGG"
     gene            1367..2068
                     /locus_tag="VNU03_03800"
     CDS             1367..2068
                     /locus_tag="VNU03_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HWP73605.1"
                     /translation="MEGAGVDVREKGANQQVSDRRLFVQLQVWTGCGDSKPLVAAVER
                     SRIEGVLYHDVNDPSGVGLLALHENPAFFATSLREALHGEPFATLRHRPELTMLGRTY
                     ASGFEPDLEDWLLARPRRTVLDPACPWGIWYPLRRAGAFARLSPQEQGAILKEHGTQG
                     RAYGEAGLAHDVRLACHGLDTHDNDFVIGLIGRELHPLSHLVQAMRRTAQTSQYLASL
                     GPFFVGHVLWQSPAR"
     gene            2065..2445
                     /locus_tag="VNU03_03805"
     CDS             2065..2445
                     /locus_tag="VNU03_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73606.1"
                     /translation="MRTLGPGALGSLLLLAALLTPAGCASLPPARQVTDMALIAGSWQ
                     GQITFARGSYQLFYLTIEPDGRVVASWDGVTRYGKVTLEGPRTRVSFYIWSGSLDYLE
                     GGGQRVILMKEDFGAWDAIVRPLS"
     gene            complement(2438..>2952)
                     /locus_tag="VNU03_03810"
     CDS             complement(2438..>2952)
                     /locus_tag="VNU03_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP73607.1"
                     /translation="GRPSSMPLTRSERERLIQQYADGPARLEGALAAVPAAARQWRPA
                     PGEWSAHEVACHCADSEVNAAARIRYVVCEPQPVVQAYDESVWATTLDYHGHSMEPAL
                     AAVRAVRAHTTALIRRLPESAWSREGRHTGSGRYTAEDWLSIYAEHLENHARQIEANV
                     AAWQAAGRPS"
BASE COUNT          387 a         1084 c         1068 g          413 t
ORIGIN      
        1 cccgccgcgg tcatcgggta ggccccgcca tgccgtccgt caaggtcgcg aaggccaccg
       61 aggtgccggt ggggcagggc ctcgtggtgc aggccggagc caagagcctc gcgctctgga
      121 acgtcgacgg ggcctatcac gtgatcgaca acacctgccc gcatcggggt gggccgatgg
      181 gggacggtga cctcgacggc cggttcgcca tttgcccatg gcacggctgg cgctgggacg
      241 tgaccaccgg agccaacgcc aacaacccgg cggtcaagat cgcctgcttc ccggcccggg
      301 tcgtcgacgg cgacgtgtac gtggagctgc cgtgatccgg gacaccctcg atcgcccgct
      361 ccgcaacctg cgcctgtccg tgaccgaccg ctgcaacctg cgctgccagt actgcatgcc
      421 cgaggaggag tacgtctggc tcccccggga cgacgtgctg accttcgagg agatggccca
      481 gctcgtcgac gtgttcaccg acctgggcgt cgacaaggtg cgcctgaccg gcggcgagcc
      541 gctgctgcgg cgggatctcg accagctggt gcgcctgctc gccggcaatg cgcggctgcg
      601 cgacctggcc ctgaccacca acggcgtgct gctggccgag cagggcgccg cgctccgcgc
      661 ggcggggctc caccgggtca cggtcagcct ggacaccctg cgggcggacc gcttccggct
      721 cctcacccgc cgcgacacgc acgcgcgggt gctcgagggc atcgcgtcgg tgggacaggg
      781 cggctggccc gggctcaagc tcgacaccgt ggtcatgcgc ggggtgaacg acgacgagct
      841 ggccgacctg gtcgagtacg cggggagcgt cggggccgag gtgcgcttca tcgagtacat
      901 ggacgtgggg ggcgccaccc actggagcat ggagcgggtg gtctcgcgac gcgagatgct
      961 cgggcgcctg agcgagcgct acggatccat cacgccgatc gagcccgtcg cggaggagga
     1021 gagcgccgcg cccgccgacc gcttccggct gcccgacggc cgcgtgttcg ggatcatctc
     1081 ctcgaccacc gagcccttct gtcgcgcctg cgatcgcagc cgcctcaccg ccgacggcct
     1141 gtggtacctc tgcctctacg cgacccgggg ggtggacctg cgcggtgccc tgcggggtgg
     1201 ggcctcgcgc gacgagctgc tgcgcctgat ccggtcgacc tggcaggggc ggcgcgaccg
     1261 cggcgcggag gagcggctgg ccctgcccga gcgccgcccg ctgatccaga tcggcgagct
     1321 caagcgcgat ccacacctcg agatgcacac gcggggagga taggccgtgg agggcgcggg
     1381 cgtcgacgtc cgggagaagg gcgccaacca gcaggtctca gaccggcgcc tgttcgtgca
     1441 gctccaggtc tggaccggct gcggcgacag caagccgctg gtggccgcgg tggagcggag
     1501 ccggatcgag ggcgtgctct accacgacgt caacgatccc tccggcgtgg gcctgctcgc
     1561 gctgcacgag aacccggcct tcttcgccac ctccctccgg gaggcgctgc acggcgagcc
     1621 gttcgccacg ctccgtcacc gaccggagct gaccatgctc ggccgcacct acgcctccgg
     1681 cttcgagccc gacctcgagg actggctgct cgcccggccc cgtcgcaccg tgctcgaccc
     1741 cgcgtgcccg tggggcatct ggtacccgct ccgccgcgcg ggcgcgttcg cgcggctctc
     1801 gccgcaggag cagggcgcga tcctcaagga gcacggcacg caggggcgcg cctacgggga
     1861 ggcgggcctg gcccacgacg tgcgcctcgc ctgtcacggc ctcgacaccc acgataacga
     1921 cttcgtgatc ggcctcatcg gccgcgagct gcacccgctc tcccacctgg tccaggcgat
     1981 gcggcgcacc gcgcagacct cgcagtacct cgcgagcctc ggccccttct tcgtgggcca
     2041 cgtgctctgg cagagccccg cgcgatgagg accctcgggc cgggggcgct cgggtcgctc
     2101 ctgctcctgg ccgccctgct gaccccggcc gggtgcgcgt cgctgccgcc cgcgcggcag
     2161 gtcaccgaca tggcgctcat cgcggggagc tggcaggggc agatcacgtt cgcccgcggc
     2221 tcctaccagc tcttctacct gacgatcgag ccggacgggc gggtggtcgc gtcctgggac
     2281 ggggtgaccc gctacggcaa ggtcaccctc gagggcccgc gcacccgcgt gagcttctac
     2341 atctggagcg ggagcctcga ctatctcgaa ggcggcggcc agcgcgtcat cctcatgaag
     2401 gaggatttcg gcgcctggga cgccatcgtc cgtcccctca gctagggcgg cccgccgctt
     2461 gccacgcggc cacgttggcc tcgatctgcc gcgcgtggtt ctccaggtgc tcggcgtaga
     2521 tggagagcca gtcctccgcg gtgtaccggc ccgatccggt gtggcgaccc tcgcggctcc
     2581 acgcggactc cggcagacgg cgaatgaggg cggtggtgtg cgcacgcacc gcccggaccg
     2641 cggcgagcgc cggctccatg gaatggccgt ggtagtcgag ggttgtcgcc cacacgctct
     2701 cgtcgtaggc ctggaccacc ggctggggct cgcacaccac gtagcggatg cgggccgccg
     2761 cattgacctc cgagtccgcg cagtggcagg ccacctcgtg cgcggaccac tccccgggcg
     2821 cggggcgcca ctggcgcgcc gccgcgggca ccgcggccag ggcgccctcc aggcgcgcgg
     2881 ggccgtcggc gtactgctgg atcaggcgct ctcgctcgga acgggtgagg ggcatcgatg
     2941 aaggcctccc tg
//
LOCUS       DATMCV010000160         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_206977, whole genome shotgun sequence.
ACCESSION   DATMCV010000160 DATMCV010000000
VERSION     DATMCV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206977"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VNU03_03815"
     CDS             <1..265
                     /locus_tag="VNU03_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HWP73608.1"
                     /translation="RVLKARLLERAQEEQAAAIAADRRTQVGTGERSERIRTYNFPQG
                     RVTDHRINLTLHRLPTVLEGDLDELIDALRERDVSQKLAESVS"
     gene            262..1191
                     /gene="prmC"
                     /locus_tag="VNU03_03820"
     CDS             262..1191
                     /gene="prmC"
                     /locus_tag="VNU03_03820"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWP73609.1"
                     /translation="MIGLVADRTVPALLARAAAALEAAGIETARPEAEWLLASLLGVD
                     RFALYLDPARRLSTPKITRYLGLIERRAAREPLQYLLGWEDFHGLRLAVSPDVLVPRP
                     ETEGLIEWALEVLAGRAEPVVADLGTGSGAIACAIARSAPSAEVLAVEISGGALAVAS
                     RNVRDLKLGARVRLLAGDLFAPLGSIPASLDLVVANPPYLPSAVIPSLPPEVSRHEPR
                     AALDGGPDGMTVLRRIVAGAPAVLKRAGWLLMEIGEEQAGPLASLMAAEGFSGIRARR
                     DLNGIERYIGGRWEPAATTLAGCASGAARREGC"
     gene            1191..>2212
                     /gene="murA"
                     /locus_tag="VNU03_03825"
     CDS             1191..>2212
                     /gene="murA"
                     /locus_tag="VNU03_03825"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWP73610.1"
                     /translation="MAARLVVQGGTPLRGDVQVSAAKNAALPALCAALLTAEPVVLEN
                     VPALADVDTTRSLLEQLGGVVSADADGSTRVHVARVTSNEAPYELVSTMRASVLVLGP
                     LLARTGLAKVALPGGCAIGVRPIDQHLKGFQKLGAEVFIRNGYVEARASRLKGARITT
                     DLVTVTGTENLMMAAALAEGTTVIENAAREPEVTDLARLLSAMGARIEGAGTERLEIE
                     GVPDLGGARHRVIPDRIEAGTLLVAAAITGGDVTVVGAEPRHMSAMLGKLEECGAVVT
                     VSGDRIRCQGPARSKAADIITSPFPGFPTDMQAQFMAMLGLADGISKVTETIFENRYM
                     HAAELSR"
BASE COUNT          278 a          805 c          844 g          285 t
ORIGIN      
        1 gcgcgtgctc aaggcccggc tgctcgagcg ggcgcaggag gagcaggccg ccgccatcgc
       61 ggcggaccgg cggacccagg tcggcaccgg cgagcgcagc gagcggatcc gcacctacaa
      121 cttcccccag gggcgggtca ccgatcaccg gatcaacctc accctgcatc gcctgcccac
      181 cgtgctggag ggcgatctcg acgagttgat cgacgcgctg cgggagcggg acgtgagcca
      241 gaagctcgcg gagagcgtgt cgtgatcggg ctcgtggcgg accgcaccgt gcccgcgctc
      301 ctcgcgcgcg cggccgccgc gctggaggcg gccgggatcg agaccgcccg cccggaagcc
      361 gagtggctgc tcgcctcgct gctgggcgtc gaccgcttcg ccctctacct cgaccccgcg
      421 cgccggctct ccaccccgaa gatcacgcgg tacctgggcc tgatcgagcg gcgggccgcc
      481 cgtgagccgc tccagtacct gctcggctgg gaggatttcc acgggctgcg gctcgcggtc
      541 tctcccgacg tgctggtgcc ccggcccgag acggaagggc tgatcgagtg ggcgctcgag
      601 gtgctggccg gccgcgcgga gccggtggtc gccgatctcg gcaccggcag tggggccatc
      661 gcctgcgcga tcgcccggtc ggcgccgagc gccgaggtgc tggcggtcga gatctccggc
      721 ggcgccctcg cggtcgcctc gcgcaacgtg cgcgatctga agctcggcgc gcgcgtccgg
      781 ctcctggccg gcgatctgtt cgcgccgctc gggtcgatcc cggcgagcct cgacctggtg
      841 gtggccaacc cgccctacct gccgagcgcg gtcatcccct cgctgccgcc ggaggtgtcg
      901 cgccacgagc cccgcgccgc gctcgacggc gggcccgacg ggatgaccgt gctccgccgg
      961 atcgtggcgg gggcccccgc ggtgctcaag cgggccggct ggctcctgat ggagatcggc
     1021 gaggagcagg ccgggccgct cgcctccctg atggcggcgg aggggttctc cggcattcgc
     1081 gcccgccgcg acctcaacgg catcgagcgt tacatcggcg ggcggtggga gccggccgcg
     1141 acgacgctcg ccggctgcgc ctcgggcgcg gcgcgccggg agggctgctg atggcggccc
     1201 ggctggtggt ccaggggggc acgccgctgc ggggcgacgt gcaggtgagc gcggccaaga
     1261 acgcggcgct gcccgcgctc tgcgccgcgc tgctcaccgc ggagccggtg gtgctggaga
     1321 acgtgcccgc gctcgccgac gtcgacacca cgcgctcgct gctcgagcag ctgggcggcg
     1381 tggtctcggc cgacgcggac ggcagcacgc gcgtgcacgt ggcccgggtc accagcaacg
     1441 aggcacccta cgagctggtc tcgaccatgc gcgcctccgt gctcgtgctg ggcccgctgc
     1501 tcgcccgcac cggcctcgcc aaggtcgccc tgccgggcgg gtgcgcgatc ggggtgcggc
     1561 ccatcgatca gcacctgaag ggcttccaga agctgggggc cgaggtcttc atccgcaacg
     1621 gctacgtgga ggcgcgggcg agccggctca agggcgcgcg catcaccacc gacctggtca
     1681 cggtcaccgg caccgagaac ctgatgatgg cggccgcgct cgcggaaggg accaccgtca
     1741 tcgagaacgc ggcgcgggag ccggaggtca cggacctggc gcggctgctc tccgcgatgg
     1801 gggcgcgcat cgagggcgcg ggcaccgagc gcctcgagat cgagggcgtt cccgatctgg
     1861 gcggggcgcg ccaccgggtc ataccggatc gcatcgaggc cgggaccctg ctggtggccg
     1921 ccgcgatcac cggcggcgac gtgacggtgg tcggggccga gccgcgccac atgtcggcga
     1981 tgctcgggaa gctcgaggag tgcggcgcgg tggtgacggt gtccggcgat cgcatccgct
     2041 gccagggccc ggcgcggtcg aaggcggccg acatcatcac gagcccgttc ccgggcttcc
     2101 ccaccgacat gcaggcccag ttcatggcca tgctcggact ggccgacggg atcagcaagg
     2161 tcaccgagac catcttcgag aaccggtaca tgcacgcggc cgagctctcc cg
//
LOCUS       DATMCV010000161         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_805291, whole genome shotgun sequence.
ACCESSION   DATMCV010000161 DATMCV010000000
VERSION     DATMCV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805291"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="VNU03_03830"
     CDS             complement(<1..1746)
                     /locus_tag="VNU03_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP73611.1"
                     /translation="MNPEAILVVDDDRDVASTVSDYLTREGHAVTVVYTGAEGLARLR
                     AGSIALLLVDLRLPDMDGTDVMRAAQRLDFPPEIVIMTGYATVDSAIQAVEGGSAGYL
                     EKPFEMPRLAGIVRRVIERRRLMQENARLQAETGSRLREMEALLSISRTASATLDVQE
                     ALRRICRELVFFVGADTGAAYLLDEPSGRLMPTAGYHVPKEMMEAFLANPIPLGDQGF
                     RQDIWEGRRPVFSDDVAADPRFTFELFRTFRHQSGLVLPLLLDDAVAGAFYFAWWKVR
                     KVFTERELVLAENVAAQVTMVLRHARLFERADREWRQLQVLYEISRALAAAEDAHEIL
                     KLLVNEATGLLGVEAAGIRLIEGDELVVRALTDSAAPLLSRARIRRGEGLSGAVVTSG
                     RPVVLADMITDERVDAADRQGAEQHGFHACLGVPLQIQGRVIGCLNVYAKSRRGFSPE
                     DISLLSVLGDHASLAIHKARLYDESRAQKEEATKLYEVTAHLATTLDVDSVLDQIVAK
                     TIDLLGCDASGVYVYDEAREGLAFRRGLHLDPVLTRDLVLKPGEGVAGRAFQERRPVS
                     TGDRHADRDLRYTPIA"
     gene            complement(1815..>2279)
                     /locus_tag="VNU03_03835"
     CDS             complement(1815..>2279)
                     /locus_tag="VNU03_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWP73612.1"
                     /translation="LGWLEEPVSAENLAGQAEVRASTDIPIAAGESEQTRFAFRDMIE
                     ARAVDICQPDVAIAGGITETQRIAALAAAHELTVAPHLWGSAILFAAGLHLAVSTPCV
                     NLLEFSRGHNPLLNDLVEERVELEDGHVLAPTGPGLGLTLKREFVERVSVRP"
BASE COUNT          326 a          791 c          824 g          338 t
ORIGIN      
        1 cgcgatcggg gtgtagcgca gatctcggtc cgcgtgccga tcgccggtgc tcaccggccg
       61 ccgttcctgg aaggctctcc ccgcgacgcc ctcccccggc ttgagcacga gatcgcgcgt
      121 gaggaccggg tcgagatgaa gtccccggcg gaacgccagg ccctcgcggg cctcgtcgta
      181 cacgtagacc cccgacgcgt cgcagccgag caggtcgatc gtcttcgcga cgatctggtc
      241 gaggaccgag tccacgtcga gggtcgtcgc caggtgggca gtgacctcgt atagcttggt
      301 ggcctcctcc ttctgcgcgc ggctctcgtc gtagaggcgg gccttgtgga tggcgaggga
      361 cgcgtggtcg ccgaggacgg agaggagcga gatgtcctcc ggcgagaagc cgcggcggct
      421 cttggcgtat acgttgaggc acccgatcac gcgcccctgg atctgcagcg gcaccccgag
      481 gcaggcgtgg aagccgtgct gctcggcgcc ctgccggtcg gccgcgtcca cgcgctcgtc
      541 cgtgatcatg tccgcgagga cgaccgggcg cccgctggtg accaccgccc cgctcagccc
      601 ctcgccccgc cggatccggg ctcggctcaa gagcggggcc gccgagtcgg tcagggccct
      661 caccacgagc tcgtcgccct cgatcagacg gatgccggcg gcctcgaccc cgagcaggcc
      721 ggtcgcctcg ttgaccagca gcttgaggat ctcgtgcgcg tcctcggccg ccgcgagcgc
      781 gcgcgagatc tcgtagagca cctggagctg ccgccactcc cggtcggccc gctcgaacag
      841 gcgggcgtgc cgcagcacca tcgtgacctg cgcggccacg ttctcggcga ggaccagctc
      901 gcgctcggtg aacaccttgc ggaccttcca ccacgcgaag tagaaggcgc ccgcgaccgc
      961 gtcgtcgagc agcagcggga gcacgagccc ggactgatgg cgaaaggtcc ggaacagctc
     1021 gaaggtgaag cgaggatcgg ccgccacgtc gtcggagaag acgggtcggc ggccctccca
     1081 gatgtcctgg cgaaacccct ggtccccgag cgggatcggg ttggccagga acgcttccat
     1141 catctccttc ggcacgtggt agcccgcggt cggcatgagc cggcccgacg gctcgtccag
     1201 gagataggcc gcgccggtgt cggccccgac gaagaacacc agctcgcggc agatccggcg
     1261 cagcgcctcc tggacgtcga gggtggcgct ggcggtgcgg gagatggaca gcagcgcctc
     1321 catctcccgg agccggctgc cggtctcggc ctggaggcgg gcgttctcct gcatgaggcg
     1381 ccggcgctcg atcacgcggc ggacgatgcc ggccaggcgg ggcatctcga acggcttctc
     1441 gagatatccc gcgctgccgc cctccaccgc ctggatggcc gagtcgacgg tggcgtagcc
     1501 ggtcatgatc acgatctcgg gaggaaagtc gagccgctgg gccgcccgca tcacatcggt
     1561 gccgtccatg tcgggcagcc gcaggtcgac gagcaggagg gcgatcgatc cggcgcggag
     1621 gcgggccagc ccttccgccc cggtgtagac gacggtgacg gcgtggccct ccctcgtgag
     1681 gtagtcgctc acggtggaag cgacatcgcg atcgtcatcg acgacgagga tggcttcggg
     1741 gttcacgcga cgagcccggc ttctacgtta gcgcaccgcg gggtttctcc ctcgttttcc
     1801 agtcgccttt gttttcaggg tcttacggag accctctcga cgaactcgcg cttgagggtg
     1861 aggccgagcc cgggaccggt gggggccagc acgtggccgt cctcgagctc gacccgctcc
     1921 tcgaccaggt cgttgaggag cgggttgtgg ccgcgcgaga actcgagcag gttcacgcac
     1981 ggggtggaga cggccaggtg caggccggcc gcgaacagga tggccgagcc ccagaggtgc
     2041 ggggccaccg tcagctcgtg ggccgccgcg agggccgcga tgcgctgggt ctcggtgatg
     2101 ccgcccgcga tcgccacgtc gggctggcag atgtcgaccg cgcgcgcctc gatcatgtcg
     2161 cggaacgcga agcgcgtctg ctcgctctcg cccgccgcga tcgggatgtc ggtggaggcg
     2221 cgcacctcgg cctggccggc caggttctcg gccgagaccg gctcctcgag ccagccgag
//
LOCUS       DATMCV010000162         6028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_8374, whole genome shotgun sequence.
ACCESSION   DATMCV010000162 DATMCV010000000
VERSION     DATMCV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8374"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VNU03_03840"
     CDS             complement(<1..889)
                     /locus_tag="VNU03_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HWP73613.1"
                     /translation="MTAPPMRFVSGEEIRRALTFPALIKAMEASHRRARMETRDVLMG
                     PEAAHYFVRSATDHGRAFGSKLIASFPGRQPAVQAIYVLFDGATGRPRAVLDGTEITC
                     WKTAADSALGASLLAPADPQTMVMLGAGAMAPWLVRAHRSVRPSLRRILVWNRTAARA
                     ETVAAGLRAEGVAAEATADLAAAVRQAQVISSATRTHTPLVEGAWLARGAHLDLVGGY
                     TPDTREADDEAARRSRVFVDCLESAFDGVGDILAPIASGAIRKEDVRGDLYDLIGGGV
                     PGRLGPDDITFFKNAGGGHL"
     gene            complement(886..2088)
                     /locus_tag="VNU03_03845"
     CDS             complement(886..2088)
                     /locus_tag="VNU03_03845"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP73614.1"
                     /translation="MRMPLEDVRVLDLSHALAGPFCSTMLADFGADVIKLEPRGAGDI
                     ARAWGSPLPGGETAYFVSLHRNKKGIEIDLKAPEGKDLFFSLVAKCDVVLENYRVGAL
                     ARLGLGYEAARERNPGIIYCSVSGFGQDGPYRDRAALDLILQAESGMISTTGEADGHG
                     VRCGVSIADMTAGMYAAFGIMLALRVKERTGRGQAIDVSMLEGQLSLLGSMIGGYFAD
                     GELPLPMGTAYKALLPYQTFRTRTRDLALAVGSEKLWKVFCPAIGCPELTDDPRYRTN
                     ADRARNRDTLIPRLQEVFLTRPYEEWEPLLLAQGIPMGAINNIAQVVEHPQVIARGAL
                     VETSHPRAGKVRMVGAPVRLSETPGSVRTPAPTLGEHTDAVLRDLLGLDAPAIASLRA
                     AGAIGPAR"
     gene            complement(2157..3398)
                     /locus_tag="VNU03_03850"
     CDS             complement(2157..3398)
                     /locus_tag="VNU03_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWP73615.1"
                     /translation="MNEDPREMVDGVYRQDSRRVLATLIRLLGDFDIAEEAMHEAFAV
                     AVEQWPRDGTPANPRAWLVSTGRFKAIDAIRRRARFDASQTELADRLAAEATDTEPRE
                     PGEIEDDRLRLIFTCCHPALPADAQVALTLREVCGLTTEEIARAFLTAPPTLAQRIVR
                     AKAKIRDAHIPYEVPSRVELPDRLDAVLHVVYLVFNEGYAASSGESLTRADLSGEAIR
                     LGRLLVELLPEPEAMGLLALMLLHESRRAARTGPTGELILLDDQDRASWSRDQIAEGT
                     RLVERALASRRIGPYTLQAAISAVHAEAPTAAATDWAQIVGLYDVLARADPSPVIELN
                     RAVAVAMRDGPTAGLALIDAILGRGALADYHLAHSARAELCRRLGRIADARTSYTRAL
                     DLARQEPQRRFLERRLRELLD"
     gene            complement(3419..3775)
                     /locus_tag="VNU03_03855"
     CDS             complement(3419..3775)
                     /locus_tag="VNU03_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWP73616.1"
                     /translation="MKFVFLIYHDEKTLDALPPKEIQTLVDGALDYDEEIRRSGHYVV
                     SNALQRARTARTVRVRGGKVSTTDGPFAETKEQLGGFFLVEAKDIDEACAVAARFPPA
                     RLGTIEVRPVQELAHS"
     gene            complement(3772..4143)
                     /locus_tag="VNU03_03860"
     CDS             complement(3772..4143)
                     /locus_tag="VNU03_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWP73617.1"
                     /translation="MKYACLVYLVEKEMEAMSESEAEACTDESLAYDEALRRSGQLIV
                     AQALAPVETATTVRVRKGKLTTTDGPFAETKEQLGGFILIEARDLNEAIQAASRIPLA
                     RRGSIEVRPIMELKPSARRIR"
     gene            4341..4700
                     /locus_tag="VNU03_03865"
     CDS             4341..4700
                     /locus_tag="VNU03_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP73618.1"
                     /translation="MIKGLSAVSVWSADLKNLLPFYRDVLGLKVVIESPRFVVLGEAG
                     GPSVGLGTHSDVRGRNADPARHMIGLTTDDLAADWKRLKAAGVEFVEAPTDYDTVSIA
                     TLKDPEGNLIQLIQRRP"
     gene            4717..5937
                     /locus_tag="VNU03_03870"
     CDS             4717..5937
                     /locus_tag="VNU03_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73619.1"
                     /translation="MKIFYGWIIVGVGIVVTCIGMGAMLSLSIFLQPMTQAMGWSRTG
                     ISTAALVNWLAMGVGAFLWGALSDRLGTRTVLLLGGGLLGLGLVCASRATTLGQFQLL
                     FGVLVGLASGSFYTPLTATTTRWFTEHRSLAVALVSAGIGLGSSTVGPLARYLITAYD
                     WRTAMFVIGNLAWLVIIPAALLVREPPGSIAAGAPGMLGADGRELTVGQALRTPQFAA
                     IALAYFACCAAHSGPIFHMVTHAIDRGITAMAAVTVLSVAGLASLGGKIVCGLIADRV
                     GAKRTLVVGLAIQALAITFYIFTRDLASFYALAVVFGFAYGGVMPLYAILVREYFGAR
                     IMGTAFGAVALVSTVGMALGPPVGGWLFDTYGSYSWLYLSSFAIGLAAVAIASTFRPP
                     GHLVPAVPSPSPAR"
BASE COUNT          792 a         2148 c         2182 g          906 t
ORIGIN      
        1 ccaggtgtcc gccgcccgcg ttcttgaaga aggtgatgtc gtcggggccg agccgtcccg
       61 gcactccgcc gccgatcagg tcgtagagat cgccgcgcac gtcctccttc cggatcgccc
      121 cgctcgcgat cggggccagg atgtcgccga ccccgtcgaa ggcggactcg aggcagtcga
      181 cgaagacgcg ggagcggcgt gccgcctcgt cgtcggcctc gcgggtgtcg ggcgtgtagc
      241 cgcccaccag gtcgaggtgc gccccgcgcg cgagccacgc gccctcaacc aggggcgtgt
      301 gggtacgtgt cgccgaggag atgacctggg cctgccgcac cgccgcggcc aggtccgcgg
      361 tcgcctcggc cgcgacgcct tcggcccgca gccccgcggc caccgtctcc gcgcgcgcgg
      421 cggtccggtt ccacaccagg atccggcgga gcgacggccg gacgctgcgg tgcgcgcgca
      481 cgagccacgg cgccatcgcg ccggcgccga gcatgaccat ggtctgcggg tcggcggggg
      541 ccagcaagct cgctccgagc gcggagtccg ccgcggtctt ccagcaggtg atctcggtgc
      601 cgtcgagcac cgcccgcggc cgtccggtgg cgccgtcaaa gagcacgtag atcgcctgca
      661 ccgccggctg gcggccgggg aagctcgcga tcagcttgct cccgaaggcg cggccgtgat
      721 cggtggccga ccgcacgaag tagtgggccg cctccggacc catcagcacg tcgcgggttt
      781 ccatgcgcgc gcggcggtgg ctcgcctcca tcgccttgat cagggcgggg aaggttaggg
      841 cccggcggat ctcctcgccg ctcacgaagc gcatcggcgg cgcggtcatc gcgcggggcc
      901 gatggcgccg gccgcgcgca gggacgcgat cgcgggcgcg tcgagcccga gcagatcgcg
      961 cagcaccgcg tcggtgtgct cgcccagcgt cggcgccggc gtacgcaccg agccgggggt
     1021 ctcggacagc cgcaccggcg caccgaccat gcggaccttg cccgcgcggg gatgcgaggt
     1081 ctccaccagc gcgccgcggg cgatcacctg cgggtgctcc accacctggg cgatgttgtt
     1141 gatggcgccc atcgggatgc cctgggcgag cagcagcggc tcccactcct cgtaggggcg
     1201 ggtgaggaag acctcctgga gccgggggat cagggtgtcg cggttgcgcg cccggtccgc
     1261 gttcgtgcga tagcgcggat cgtcggtcag ctccgggcag ccgatcgcgg ggcagaacac
     1321 cttccagagc ttctcgctgc ccaccgccag ggccaggtcg cgggtcctgg tgcggaaggt
     1381 ctggtacggc agcagcgcct tgtacgcggt gcccatcggc aggggcagct cgccatcggc
     1441 gaagtagccg ccgatcatgc tgccgaggag cgagagctga ccctccagca tcgagacgtc
     1501 gatggcctgc ccgcgcccgg tccgctcctt cacccgcagc gcgagcatga tcccgaacgc
     1561 ggcgtacatg cccgcggtca tgtcggcgat ggagacgccg cagcgaacgc cgtggccgtc
     1621 cgcctccccg gtggtgctga tcatcccgct ctcggcctgg aggatgaggt cgagcgcggc
     1681 gcggtcgcgg tacgggccat cctggccgaa gcccgagacc gagcagtaga tgatgccggg
     1741 gttacgctcg cgggcggcct cgtagccgag gccgagccgg gccagggcgc cgacgcggta
     1801 gttctccagc accacgtcgc acttggccac cagagaaaag aagaggtctt tcccctcggg
     1861 ggccttcaga tcgatctcga tgcccttctt gttgcggtgc aggctgacga agtaggcggt
     1921 ctcgccgccc gggagcgggg acccccacgc gcgcgcgatg tcgccggcgc cgcggggctc
     1981 gagcttgatc acgtcggcgc cgaagtcggc gagcatcgtg gagcagaagg ggccggccag
     2041 ggcgtggctc aggtcgagca ccctcacgtc ttcgagcggc atgcgcatcg atcggcctcc
     2101 gggcgcgttt actatgcgcc gcgctccacc ggaggatcct gccagatcgc cgcgcgtcag
     2161 tccagcagtt cgcggaggcg ccgctcgagg aagcgccgct gcggctcctg ccgcgcgagg
     2221 tcgagggcgc gcgtgtaaga ggtgcgggca tccgcgatcc ggccgagccg ccggcacagc
     2281 tccgcgcgcg cggagtgcgc gaggtgatag tccgccagcg cgccgcggcc gaggatcgcg
     2341 tcgatgaggg cgagccccgc ggtggggcca tcgcgcatgg ccaccgccac cgcgcggttc
     2401 agctcgatga ccggagaggg gtcggcccgc gccagcacgt cgtagaggcc gacgatctgg
     2461 gcccagtccg tcgcggccgc ggtgggggcc tcggcgtgca ccgccgagat cgcggcctgc
     2521 agcgtgtagg ggccgatgcg acgcgaggcc agcgcccgct ccaccagccg ggtcccctcc
     2581 gcgatctggt cgcggctcca gctcgcacgg tcctggtcgt cgagcaggat cagctcgccg
     2641 gtcggcccgg tgcgcgcggc ccgccgcgac tcgtgcagca gcatcagggc cagcaggccc
     2701 atcgcctccg gctccggcag cagctccacc aggagccgtc ccagccggat ggcctcgccg
     2761 gagagatccg cgcgcgtgag cgactcgccc gacgaggcgg cgtagccttc gttgaacacc
     2821 aggtagacca cgtgtagcac cgcgtccagg cggtcgggca gctcgacgcg cgagggcacc
     2881 tcgtagggga tgtgcgcgtc gcggatcttg gccttggcgc ggacgatgcg ctgggccagg
     2941 gtcggcggcg cggtgaggaa ggcgcgcgcg atctcctcgg tggtgagccc gcagacttcc
     3001 cggagcgtga gcgcgacctg ggcgtccgcg ggcagggcgg ggtggcagca ggtgaagatc
     3061 aggcggagcc gatcgtcctc gatctcgccg ggctcgcgcg gctcggtgtc ggtggcctcc
     3121 gcggcgagac ggtcggccag ctcggtctgc gaggcgtcga accgggcccg ccggcggatc
     3181 gcgtcgatcg ccttgaaccg cccggtcgac acgagccacg cgcggggatt ggccggggtg
     3241 ccgtcgcgcg gccactgctc caccgcgacc gcgaaggcct cgtgcatcgc ctcctcggcg
     3301 atgtcgaaat cgcccaggag gcggatcagg gtcgcgagga cgcggcgcga gtcctgccga
     3361 tagacgccgt ccaccatctc gcgcggatcc tcgttcatgg acgtgagtgc gcgcgcgcct
     3421 atgagtgcgc cagctcctgg accggtcgca cctcgatggt ccccagccgt gccggcggga
     3481 agcgggcggc caccgcgcag gcctcgtcga tgtccttggc ctcgacgagg aagaagccgc
     3541 ccagctgctc cttggtctcc gcgaaggggc cgtcggtggt ggacaccttg ccgccgcgga
     3601 cccggacggt ccgggcggtc cgcgcgcgct gcagcgcgtt cgagacgacg tagtgcccgc
     3661 tccggcggat ctcctcgtcg tagtcgaggg cgccgtccac gagggtctgt atctcctttg
     3721 ggggcagggc gtcgagggtc ttctcgtcgt gatagatcag gaacacgaac ttcatcggat
     3781 cctcctcgcg gacggcttca gctccatgat cggccgcacc tcgatggacc cccggcgcgc
     3841 cagcgggatc cgcgacgcgg cctggatggc ctcgttgagg tcccgggcct cgatcaggat
     3901 gaagccgccc agctgttctt tggtctcggc gaacgggccg tcggtggtgg tcagcttgcc
     3961 cttccggacc cgcaccgtcg tcgcggtctc gaccggtgct aaggcctggg ccacgatgag
     4021 ctgtccgctc ctccggagag cctcatcgta cgccaggctc tcgtcggtgc aggcctccgc
     4081 ctcgctctcg gacatcgcct ccatctcctt ctccaccagg tagaccaggc acgcgtactt
     4141 catgggcccc tcctcggcgc attgaatggg gtgcggacac cggatagtcg cccggagcgc
     4201 ccggaatcga cacccacctc cgcccttgcc aagcatgccg cccctcccgg atgctgtcga
     4261 tcgcgggcgg ccccattcga tcatgtagtg aagccggtaa cgaagccggg cgcgagcccc
     4321 aaccccataa ggagaccacg atgatcaagg gattgagcgc ggtgtcggtc tggagcgcgg
     4381 atctgaagaa cctgctgccg ttctaccgcg acgtgctggg gctcaaggtg gtgatcgagt
     4441 caccgcgctt cgtcgtcctg ggcgaggccg gggggcccag cgtcgggctc gggacccaca
     4501 gtgacgtccg gggccgcaat gccgacccgg cgcggcacat gatcggcctg accaccgacg
     4561 acctcgcggc ggactggaag cgcctgaagg cggcgggggt cgagttcgtg gaggcgccca
     4621 ccgactacga caccgtctcg atcgcgaccc tgaaggaccc cgagggcaac ctgatccagc
     4681 tcatccagcg gcgtccgtga tagggtgagg cccgccatga agatcttcta cggctggatc
     4741 atcgtgggcg tggggatcgt cgtcacctgc atcggcatgg gggcgatgct ctcgctcagc
     4801 atcttcctcc agccgatgac ccaggcgatg gggtggtccc ggaccgggat ctcgaccgcg
     4861 gccctggtga actggctcgc gatgggcgtc ggggcattcc tctggggcgc gctctccgat
     4921 cggctcggga cccgcaccgt cctgctcctg gggggcggtc tcctcgggct cgggctcgtc
     4981 tgcgcgagcc gggccacgac gctcggccag ttccagctcc tgttcggggt gctggtgggg
     5041 ctcgcctcgg gcagcttcta cacgcccctg accgccacca ccacccgctg gttcacggag
     5101 caccggagcc tggcggtcgc gctggtctcg gccggcatcg ggctcggctc ctccaccgtc
     5161 gggcccctgg cgcgctacct gatcaccgcc tacgactggc gcaccgcaat gttcgtgatc
     5221 ggcaacctgg cgtggctggt catcatcccc gccgccctgc tggtgcgaga gccgcccggc
     5281 tcgatcgccg cgggcgcccc cgggatgctt ggggcggacg ggcgcgagct caccgtcggg
     5341 caggccctgc gcacgcccca gttcgcggcc atcgcgctcg cgtacttcgc ctgctgcgcg
     5401 gcccactcgg gaccgatctt ccacatggtg acccacgcga tcgatcgcgg gatcaccgcg
     5461 atggccgcgg tcaccgtgct gagcgtggcg gggctcgcct cgctcggcgg caagatcgtc
     5521 tgcgggctga tcgccgaccg cgtcggcgcc aagcgcaccc tggtggtggg gctcgcgatc
     5581 caggcgctgg cgatcacgtt ctacatcttc acccgcgacc tcgcgagctt ctacgccctc
     5641 gcggtggtgt tcggcttcgc ctacggcggg gtcatgccgc tgtacgcgat cctggtgcgc
     5701 gagtacttcg gggcccgcat catggggacc gcgttcgggg cggtcgcgct cgtctccacc
     5761 gtcggcatgg cgctcgggcc gccggtgggc ggctggctct tcgacacgta cggcagctac
     5821 tcgtggctct acctgagctc gttcgccatc ggtctggccg cggtggccat cgcctccacg
     5881 ttccgcccgc cgggccacct cgtccccgcg gtgccgagcc cgagcccggc ccgctgaggg
     5941 cagggcactc cgcgatgcgc ttcctgctgc tcatgtaccg tgaggaacgg atctgggacg
     6001 ccaagcccga ggcggagcgg gtcgcgat
//
LOCUS       DATMCV010000163         4178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_341328, whole genome shotgun sequence.
ACCESSION   DATMCV010000163 DATMCV010000000
VERSION     DATMCV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341328"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1689
                     /locus_tag="VNU03_03875"
     CDS             40..1689
                     /locus_tag="VNU03_03875"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWP73620.1"
                     /translation="MARGRGGRFLRLTAGLVVAVAMSGVGLCPEPALAQPADVRVLTL
                     RVVAEEMYRARPGWDAELRRTVKTVSDIYERNFQIRFQIQEVVPWTIGPAVPLQTIMT
                     RLHREMAVGSADVLVGFAAERCEGLHYGVAQLFGRVAMVQTSCLDTAVLSNTTAEAVL
                     SHEIGHLFGAFHPGPSGAGSVMRYGPAGVFDQQNARIIRMMRARDFTTGVRGVDEAMR
                     KTWSAIYAEGHASDEPNPLAGAIANAGAALAAEGKMPEAEALIREAVGVDPSAARVHS
                     MLGLLLSEQGRLPEAASELQTAKRLSFRETDARTELGFVLLKLGREEEALVELRDVLR
                     VDRRAPRPYVGVGMILARRNRVPEAISALRTAIAVDPTYGPAQLELAGVLDTVGQPAE
                     AWAAAERAHDLGEDVPSALWQRLAAKVPTAPPLPPTCAGAPGPAIDLDRPDAASRAYV
                     TKVRERIQAHHVYPRVAGEARQAGELQIELQLARSGRLDCLALRRSSGSEILDRYILT
                     AVRLAQPFAPIPSYMPQPSLTLSGVFSYSVGAEPKAPPPPK"
     gene            1742..4048
                     /locus_tag="VNU03_03880"
     CDS             1742..4048
                     /locus_tag="VNU03_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWP73621.1"
                     /translation="MADAPIATLESIQKRVLWLSALMVHHANSTRPNPDGTKVGGHQA
                     SSSSVAGLMTALYFHALRPGDIVATKAHASPILYAIEYLRGRLSAEQLRGLRSLGVLQ
                     AYPSRRKNPEIIDLSTGSMGLGAVSAAFGALAARYVSQHGGGAMPARFIALVGDAELD
                     EGNVWEGLLEEAVRELDNLLWIVDVNRQSLDRVVPDARRAQLRDWFRGAGWHVIELRW
                     GGRLRARFGWPGGERLRARLEGLGAAEYQALLRLPAGAARKAVIGTPEGETDPAVDRL
                     LADVPDETLGALLADVGGHDLAAILAAYDEAAQPRRGPCVILADTIKGWGYPFAADQM
                     NHGALLTQRQLDELRESLGVDPADEWGGFPAGSAEAALVRGAPAPYAPPAVEPAPEVP
                     DTLEETYPPQSSTQEAFGRVLGRLGRVPIGDRVVTFSADVSVTTHLAGWITRKGVYFP
                     RVKPGFAAEGPAMVQWKESPAGQHVELGIAEHNLFLALGAFGLAHELSGRTLLPIGTL
                     YDPFVSRGLDALYHALYAGGRFIVAATPSGVSLSPEGGAHQSVITPGIGVALPAITYY
                     EPTFAQDVEWILLDGLRGLLRRGDAESLYLRLSTKPIDQALAPAPSPAYREAVRRGGY
                     RLIDAREQPGYDPEGNAVHIFAAGVMVPEAVAASRALAATGICASVFAATSPDLLYRG
                     LRATRPYVLDLVSADEEGVPVVSVLDGHSHALAFLGSALGVPQLPLGVDDFGQSGARG
                     DVYRHYGVDADAIARAAHSLLARGPALG"
BASE COUNT          529 a         1524 c         1547 g          578 t
ORIGIN      
        1 cgacatggcc cgacaggcgg cccggttcat ggagtacgtg tggcacgagg acgcggggga
       61 cgattccttc gcctgaccgc gggtctcgtg gtggccgtcg ccatgtccgg ggtcggcctg
      121 tgtcctgagc cggcgctcgc ccagcccgcc gacgttcgcg tcctcacgct ccgggtcgtg
      181 gccgaggaga tgtaccgggc ccgccccggc tgggacgccg agctgcgccg caccgtcaag
      241 accgtctccg acatctacga gcggaacttc cagatccgct tccagatcca ggaggtggtg
      301 ccgtggacga tcggtcccgc ggtgccgctg cagaccatca tgacccggct gcaccgcgag
      361 atggcggtcg ggtccgccga cgtcctggtg gggtttgcgg cggagcggtg cgagggcctg
      421 cactacggcg tcgcgcagct cttcggtcgg gtggcgatgg tgcagaccag ctgcctcgat
      481 accgcggtcc tgtccaacac aaccgccgag gcggtgctct cgcacgagat cgggcatctc
      541 ttcggcgcgt tccatcccgg gccgtcgggc gccggctcgg tcatgcgcta tggccccgcc
      601 ggtgtcttcg accagcagaa cgcccggatc atccgcatga tgcgtgcgcg tgacttcacg
      661 actggcgtgc ggggagtgga tgaggcgatg cgcaagacgt ggagcgcgat ctacgcggag
      721 ggacacgcct cggacgagcc caacccgctg gccggcgcga tcgccaacgc gggcgccgcg
      781 ctggccgcgg aaggtaagat gccggaggcg gaggccctga tccgcgaggc cgtcggggtc
      841 gatccgtccg cggcgcgggt acacagcatg ctgggccttc tcctgagcga gcagggccgc
      901 ctgccggagg cggcgagcga gcttcagacc gcgaagcggc tgagcttccg ggagacggac
      961 gcgcgcaccg agctgggctt cgtgctgctc aagctcgggc gtgaggagga ggcgctcgtc
     1021 gagctgcgcg acgtgctccg cgtcgatcgg cgggcccctc ggccgtacgt cggggtcggc
     1081 atgatcctcg cccggcggaa ccgcgttccg gaggcgatca gcgcgctccg caccgcgatc
     1141 gccgtcgacc cgacgtacgg gccggcccag ctcgagctgg ccggggtgct cgacaccgtc
     1201 ggccagccgg ccgaggcgtg ggccgcggcg gagcgggcgc acgacctggg cgaggacgtg
     1261 ccgagcgcgc tctggcagcg cctggccgcg aaggtgccga ccgcgccgcc gctgcctccg
     1321 acgtgcgccg gggcgccggg gccggcgatc gacctggacc gtccggatgc ggcgtcccgc
     1381 gcgtacgtga cgaaggtgcg cgagcgcatc caggcccacc acgtctaccc gcgggtggcc
     1441 ggcgaggccc ggcaggcggg cgagctgcag atcgagctgc agctcgcgcg gagcgggcgg
     1501 ctcgactgcc tggcgctgcg gcggtcgtcc ggctcggaga tcctcgaccg ctacatcctg
     1561 accgcggtcc ggctggccca gccgttcgct cccatcccgt cctacatgcc gcagccgtcg
     1621 ctgaccttga gcggcgtgtt cagctactcc gtcggcgccg agccgaaagc gccgccgccc
     1681 ccgaagtaga cgcagcacgg cctctggtcc gtcccgccgc cctgcggtaa ggtacgacga
     1741 catggccgac gcccccatcg ccacgctcga gtcgatccag aagcgcgtcc tctggctcag
     1801 cgcgctcatg gtccaccacg ccaacagtac gcgcccgaat cccgacggca ccaaggtggg
     1861 cggccaccag gcctcgtcct cgtcggtggc ggggctcatg accgcgctct acttccacgc
     1921 gctgcggccg ggcgacatcg tggccaccaa ggcccacgcc tcgccgatcc tctacgccat
     1981 cgagtacctg cgcgggcgtc tctccgccga gcagctccgc gggctgcgca gcctgggcgt
     2041 gctccaggcc tacccgagcc ggcggaagaa tcccgagatc atcgatctct cgaccggctc
     2101 catgggcctg ggggcggtga gcgcggcctt cggcgccctc gccgcgcgct acgtgagcca
     2161 gcacggcggg ggagcaatgc cggcccgctt catcgccctg gtcggcgacg cggagctgga
     2221 cgagggcaac gtctgggagg gcctcctcga ggaggcggtg cgggagctcg acaacctgct
     2281 ctggatcgtc gacgtgaacc gccagagcct cgaccgcgtg gtgcccgacg cgcgacgggc
     2341 ccagctgcgc gactggttcc gcggcgcggg ctggcacgtg atcgagctgc gctggggcgg
     2401 ccggctgcgc gcgcggttcg gctggccggg cggcgagcgg ctgcgcgcgc ggctcgaggg
     2461 cctgggtgcc gcggagtacc aggcgctgct gcgcctcccc gcgggcgccg cgcgcaaggc
     2521 ggtgatcggc acgcccgagg gcgagacgga ccccgcggtg gatcggctgc tggccgacgt
     2581 gcccgacgag accctgggcg ccctgctggc cgacgtgggt ggccacgacc tggccgcgat
     2641 cctcgccgcc tacgacgagg cggcgcagcc gcggcgcggg ccctgcgtga tcctggccga
     2701 caccatcaag ggctggggct atccgttcgc ggcggaccag atgaaccacg gcgcgctgct
     2761 cacccagcgg cagctcgacg agctgcggga atcgctcggc gtcgatcccg cggacgagtg
     2821 gggcggcttc ccggccggca gcgcggaggc ggcgctcgtg cgcggggcgc ccgcgcccta
     2881 cgcgccgccc gccgtcgagc ccgcgcccga ggtgcccgac acgctggagg agacctatcc
     2941 gccgcagtcc tcgacgcagg aggcgttcgg tcgcgtcctc gggcgcctgg ggcgggtgcc
     3001 gatcggcgac cgcgtggtga ccttctcggc cgacgtgtcg gtgaccaccc acctggcggg
     3061 ctggatcacc cgcaagggcg tgtactttcc acgggtgaag ccgggcttcg ccgcggaggg
     3121 cccggcgatg gtgcagtgga aggagtcgcc ggccggccag cacgtcgagc tcggcatcgc
     3181 cgagcacaac ctgtttctcg cgctcggggc cttcggcctc gcccacgagc tgtcgggccg
     3241 gacgctcctg cccatcggca cgctctacga tcccttcgtg agccgcgggc tcgacgcgct
     3301 ctaccacgcg ctctacgcgg gcgggcgctt catcgtggcc gccaccccgt cgggggtgag
     3361 cctctcgccg gagggcggcg cgcaccagtc ggtgatcacg cccggcatcg gcgtggcgct
     3421 gcccgcgatc acctactacg agcccacctt cgcccaggac gtggagtgga tcctgctcga
     3481 cgggctgcgt ggcctgctgc ggcggggcga cgcggagagc ctctacctgc ggctctccac
     3541 caagccgatc gaccaggcgc tggcgcccgc gccgtcgccc gcgtatcgcg aggcggtgcg
     3601 gcggggtggc taccggctga tcgacgcgcg cgagcagccc ggctacgatc cggagggcaa
     3661 cgcggtgcac atcttcgcgg ccggcgtgat ggtgcccgag gcggtggcgg cgagccgcgc
     3721 gctggccgcg accgggatct gcgcgagcgt cttcgcggcc acgagtccgg acctgctcta
     3781 ccgcggcctg cgcgctacgc gcccctacgt actcgacctc gtgtcggcgg acgaggaggg
     3841 cgtgccggtg gtgtcggtgc tggacggcca ctcccacgcg ctggccttcc tgggtagcgc
     3901 gctcggcgtc ccgcagctgc cgctcggggt ggatgacttc ggccagtcgg gcgcgcgagg
     3961 cgatgtctac cgccactacg gcgtcgacgc cgacgcgatc gcgcgcgccg cgcacagtct
     4021 ccttgcccgg ggccccgccc tgggttagca tccgagcatc ccagccgcgc tccgtagcgc
     4081 catcgcgctc caaaggagga gcctccatgg acagccgcct caagaatctg gccgagcagc
     4141 tcgacgcagg cctcatcgcg cgccgtgagt tcctgcgt
//
LOCUS       DATMCV010000164         2384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_541109, whole genome shotgun sequence.
ACCESSION   DATMCV010000164 DATMCV010000000
VERSION     DATMCV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541109"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VNU03_03885"
     CDS             <1..543
                     /locus_tag="VNU03_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWP73622.1"
                     /translation="LVPMAGAVFMALPDSEAAAWHHGTLATRGDEYDAGTRRRLVTAA
                     LLPATLGQQAARARAAIRTQLWDALGRFDVLLAPTAPTAAPPIASAQAPISSRAEAGR
                     RFFNRRSYTTPASLAGVPALSLPCGFTAGGLPIGLQLIGRRFDEVTLLRAGRAYERAT
                     DWHAAGRPCPLDSGPLPVVR"
     gene            551..877
                     /locus_tag="VNU03_03890"
     CDS             551..877
                     /locus_tag="VNU03_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007631202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HWP73623.1"
                     /translation="MRAIGEIATFVTSKNAGPFLLTLDIVFPDEATFRRFVAGRPLDR
                     ATVARLYGLSPDDVVDIIEFEPAFAVKITLRRPWPSGAIGETDVYGAQQHVPLARHAI
                     EWPESA"
     gene            877..2226
                     /locus_tag="VNU03_03895"
     CDS             877..2226
                     /locus_tag="VNU03_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HWP73624.1"
                     /translation="MTPPMTVLAPLGMLGYGIPARSMERGLARDPAVLALDAGSTDPG
                     PYYLGAGVPFVNRRAAKRDLTMILDAARDRGIPVLIGSAGGGGGRPHLEWTLDLYREL
                     CRERGYRFRTAVIDAEVDRGWLRAQVAGGRVRPLDHDRALTAADVDQAARIVAQMGVE
                     PFLAALDAGAEVIIAGRACDAAVIAAFPIRAGHDPGLALHMGKILECGGAAAYPRHGS
                     DCLLGVIEEDAFTVEPPNPDKVCTVASVAAHSLYERANPYELHLPHGHIDLKGTRFEA
                     SGDRAVRVSGTRFVPAATPTLKLEGVARVGFRTLAIAGVREPQLIANLDAYLDNVRTR
                     VADTYPDGYRLMFHVYGRDGVMGPLEPVRGSRPHEIGLIIEVIADDAETSAAVLALAR
                     SVALHVTYPGRQAIAGNLAFPFSPSDLRAGEAYEFTIHHLVAIDDPGALFRVEMLAG"
BASE COUNT          285 a          953 c          841 g          305 t
ORIGIN      
        1 ctcgtgccga tggcgggcgc ggtgttcatg gccctgcccg acagcgaggc cgcggcctgg
       61 caccacggga cgctggccac gcgcggggac gagtatgacg cgggcacgcg acgccggctc
      121 gtcaccgcgg cgctgctgcc cgccacgctc ggccagcagg ccgcgcgggc gcgcgcagcg
      181 atccggacgc agctctggga cgcgctcggg cgcttcgacg tgctgctggc ccccaccgcg
      241 ccgaccgccg cgccgccgat cgcgagcgcc caggcgccca tctcctcccg cgcggaggcc
      301 ggccggcgct tcttcaaccg ccgctcctac accacccccg cgagcctggc cggcgtcccc
      361 gcgctctcgc tgccgtgcgg gttcaccgcg ggcggcctgc cgatcgggct ccagctcatc
      421 ggccgccgct tcgacgaggt gaccttgctg cgcgccgggc gcgcgtacga gcgcgcaacc
      481 gactggcatg ccgccggccg cccctgcccg cttgacagcg ggccgctccc ggtggtgcga
      541 tgacggcggg atgcgcgcca tcggggagat cgccacgttc gtgaccagca agaacgcggg
      601 gccgttcctg ctgaccctcg acatcgtgtt cccggacgag gccacctttc gccgcttcgt
      661 ggcggggcga cccctcgacc gcgccacggt cgcgcggctc tacggtctct cgcccgacga
      721 cgtggtcgac atcatcgagt tcgagccggc cttcgcggtg aagatcaccc tgcgccggcc
      781 gtggccctcc ggcgcgatcg gcgagaccga cgtgtacggg gcccagcagc acgtccccct
      841 ggcccgccac gcgatcgagt ggccggagag cgcgtgatga cgccgccgat gacggtgctg
      901 gccccgctcg gcatgctggg ctacggcatt cccgcgcgct cgatggagcg cggcctcgcg
      961 cgcgatcccg cggtcctcgc gctcgacgcg ggctcgaccg accccgggcc gtactacctg
     1021 ggcgcgggcg tgccgttcgt caaccgccgc gcggccaagc gcgacctcac gatgatcctc
     1081 gacgccgcgc gcgaccgcgg catcccggtg ctgatcggct ccgcgggcgg cggcggcggg
     1141 cggccgcacc tcgagtggac gctcgacctc taccgcgagc tctgccgcga gcgcggctac
     1201 cgcttccgca ccgcggtgat cgacgcggag gtggaccgcg gctggctccg cgcccaggtg
     1261 gcgggcggcc gggtgcgccc gctcgaccac gaccgcgcgc tgaccgccgc cgatgtcgat
     1321 caggccgccc gcatcgtggc ccagatgggc gtcgagccct tcctcgccgc actcgacgcg
     1381 ggcgcggagg tgatcatcgc cggccgcgcc tgcgacgccg cggtcatcgc ggccttcccg
     1441 atccgcgccg gccacgatcc cggcctcgcg ctccacatgg gcaagatcct cgagtgcggc
     1501 ggcgcggccg cctacccgcg gcacggctcg gattgcctgc tcggcgtcat cgaggaggac
     1561 gccttcacgg tcgagccgcc gaacccggac aaggtgtgca cggtggcctc ggtggctgcg
     1621 cactcgctct acgagcgcgc caatccctac gagctgcacc tgccccacgg ccacatcgat
     1681 ctcaagggca cgcgcttcga ggcttccggc gatcgggcgg tgcgcgtctc gggcacgcgc
     1741 ttcgtccccg cggccacccc gaccctgaag ctcgaggggg tggcccgcgt cggcttccgc
     1801 accctcgcga tcgcgggggt gcgcgagccg cagctcatcg ccaacctcga cgcctacctc
     1861 gacaacgtgc gcacgcgcgt ggccgacacc tatcccgacg gctaccggct catgttccac
     1921 gtctacgggc gcgacggggt catgggtccg ctcgagccgg tgcgcgggag ccggccgcac
     1981 gagatcgggc tgatcatcga ggtgatcgcc gacgacgcgg agacctccgc cgcggtgctg
     2041 gccctcgcgc gctcggtggc gctccacgtc acctaccccg gccgccaggc gatcgcgggc
     2101 aacctcgcgt tccccttctc gccctccgac ctgcgcgcgg gcgaagccta cgagttcacg
     2161 atccaccacc tggtggcgat cgacgacccg ggcgcgctct tccgcgtgga gatgctcgcg
     2221 gggtgacccc gggcgccggg cgcgccgcgg cggcgcggcg cgcgctcagc gcagcaccgc
     2281 gatcgcctgg ccgcccgccg ccgcgaaggc caccaccacc cagggcggca ggctccacca
     2341 ggccagcagc ccgaaggcgg cgagggccag cgcgaagtcg gcgg
//
LOCUS       DATMCV010000165        10675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_76314, whole genome shotgun sequence.
ACCESSION   DATMCV010000165 DATMCV010000000
VERSION     DATMCV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10675
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76314"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1520)
                     /gene="pelG"
                     /locus_tag="VNU03_03900"
     CDS             complement(105..1520)
                     /gene="pelG"
                     /locus_tag="VNU03_03900"
                     /inference="COORDINATES: protein motif:HMM:NF028243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HWP73625.1"
                     /translation="MAGIGFRLKRLIVEEGGTGWLRAHLYGAVLSAGPWLLSICTLGS
                     LALLSRDLIGDLAHARFQVIVVYTYTFSLVTTGAAHMVVTRQLADELYVGRLGALVVS
                     YRVTIAATALAHLALGTLVYALAPGLEPALRLFGVMLLVVVSCTWMVMIYLGAAQDYA
                     SVVTAFFLGNAVSLLGALALGRYLGTSGYLAGFLVGQAGIFFVLCARVEREFSLVRVP
                     ESVDLTRAFVHYPELIAAGLLYNAAIAVDRVVFWIGPTGHPIVGWFRASLYDSPIFLC
                     YLSVVPALAIFLVSVETDFYDRYREYYGVATKHGTLRQVLEAKRAMTACLRDSLRRLL
                     VAQAPVTLLLMALAPWIADGLGLDRLQVSILRCGLVGAVLHVLCLFGGIVLLYFDRRR
                     AAAEVAGVFLAANLLLTVATLVVGPRAYGLGYPLAALLGCAWAYRRLEQTLADLEYLT
                     FAAQPMVPEAPAVESSHPASA"
     gene            complement(1534..3024)
                     /gene="pelF"
                     /locus_tag="VNU03_03905"
     CDS             complement(1534..3024)
                     /gene="pelF"
                     /locus_tag="VNU03_03905"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HWP73626.1"
                     /translation="MSERPADICLILEGTYPYVTGGVSSWIHQLVNALPELRFAIFHI
                     SATQGEAHEPRYKMPPNVVSLVDVGIHGGDEPALKGEVTEAAAWETIWRYHEEMKDGR
                     ADGLEGLLRQISPHAGAGPSVHDFIYGKPSWEIVRRLYESRAPDISFLDYFWTWRFTH
                     LPMFRLLHAPVPEAAVYHTVSTGWAGFVAAVQRVRRGRPMLLTEHGIYSRERRMEIDQ
                     AEWIYVQRQAPMTLSAGPGFFKELWARLFERLSRITYVQADRIVTIFEGNRQAQIRDG
                     ADPAKTLVVPNGLDVGPLSALPRVEPAAGEFRIGFVGRVVPIKDVKTFIRAVKIVVTA
                     HPGASAVIAGPGDEDPEYLAECQALAATLGISERVEFTGRVDVKQIYPRVDVLVLTSI
                     SEGLPLVILEAAAAGIPVVSTDVGACRELLEGRLPADRALGASGLVTGLADPAATARA
                     ILSLARDPGLCEEMGRAARARVRAHYQESDLIRRYREIYADLLGAA"
     gene            complement(3021..3974)
                     /locus_tag="VNU03_03910"
     CDS             complement(3021..3974)
                     /locus_tag="VNU03_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73627.1"
                     /translation="MTRALAGLAELVAVGAALASWWPLALLAHVAAAWLAARGLESGR
                     RDERLLVVALVLALPVLGLVGLGAIRVWQRRAPPSGLYAGAHSEMAGLPGPGQAPEPV
                     DRVFEWLQAQVSVQPVADLIRAADPRMQRWGIGLLAKRGDGAAVELLREALQAPDRDT
                     QIAASGALQRVEERLAQRIGQAQEQRRLDPESAERAMAMGDACLAYQESGLLDAAMGR
                     HWLGEAEAAYRAARARRPDWPPPARALARVLLALGRIDEAEHLAVEARAAAPSTEIDL
                     LLSEILFRQGRWRELQEVSRDAVAAGRADELLRWWAGEPAA"
     gene            complement(3971..5323)
                     /locus_tag="VNU03_03915"
     CDS             complement(3971..5323)
                     /locus_tag="VNU03_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP73628.1"
                     /translation="MLAALRRAQPYLLELGGGLLLLVVVNAVLRPRDPGFLDVQPNPA
                     LVLVVVLAGRHGLRAGLVSGLVTAAAVAACVAVRMDHASWSELRSLPRYVTPLLLIGT
                     GFGLGALRETQLRRTRALEAQVEGLEQELADQAVRFMAATEAKHELERRVADERASLS
                     SLYTAARALETLDAERLYPAIAATARRFLQADACQCYVLDGDRLRLRAAEGPAPERAE
                     IAPDDGLVGLAVRLGRPVSVRDYTTIGSLEDLQGAELLMAAPMTSAEGALLGCITVTK
                     LPFLRLTPVSLDRLGLVADWGARTLENARTHEQTLARTIEDELVRAYTYAYYQRRIHE
                     EEVRADRYGRPLAVVIFRIHRFGAVAADRRADLGRVLSLVFSRTLRDVDIVCRYATED
                     AFAIILPETAPTRAEVVVDRLSSEIRNFHFAPYADDEQDLEFSARVLAVRERPGGVTS
                     "
     gene            complement(5331..5897)
                     /locus_tag="VNU03_03920"
     CDS             complement(5331..5897)
                     /locus_tag="VNU03_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HWP73629.1"
                     /translation="MWRRSRVVVLAIGVLTLIGCASTVQDWVKPGAARVTRVAVLPLE
                     NQTSSLRAGQVASDLLVSEILATGAMTVMDPSEVADLLRRENLDPADAGRLPSAQRVG
                     RLLQVSHVLQGSVTEYRYKPGLSETPAVGVTARLVDVASGEVVWTSSHARLGSSWLRE
                     DGLARVAQRVAHDMAVHLTAALGDPRAR"
     gene            complement(5906..7390)
                     /locus_tag="VNU03_03925"
     CDS             complement(5906..7390)
                     /locus_tag="VNU03_03925"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP73630.1"
                     /translation="MARLRSLRLASVLIAGLLALAPAPGRAQAPAPGASETPSAPATE
                     PPTIDAPEAETLRARIQARLGLVEEALGTYRALLARYPDDRRLREDYAEVMVDAGLLD
                     QAAPVVDRYLAEDPTSARLRRLRARIDLAAGAPAAAGRRLDALAREQPRDTGLTADLA
                     AAELAAGRWNRALDLYGRLLDGDPENRDALAAYRDILLGHAPRIELTHYSLLQQAATH
                     HVEEAAWRGWLSDRWWLRAGTRYGSYHQERVIGQPSFTEEVRTALATVGFQPTRAVSL
                     WGGLEESERREDIFRTTGRLGAGYDDQRATLATLEVAVRELLTNPVAAVPRSGTTDRV
                     TVDVARRVLVPVVLAAHYDFRHYRISDEDLGNRWEAAARAEIELVRSRVQVTLIPQVF
                     FSEYKPAAGSPLRDAVTFLRREDIVAIGALVGWDVTPALRLQVGSVGRRDLHRSLTSW
                     EVTGEGRWRIRPWLEGRVLYTRNTESTTVGGQEESFLGRLDILY"
     gene            complement(7451..9670)
                     /locus_tag="VNU03_03930"
     CDS             complement(7451..9670)
                     /locus_tag="VNU03_03930"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP73631.1"
                     /translation="MSIERWKLTLAVVVTLGLLALSVPWGADLGWAYLADRQYALAER
                     MFARALDGDRGDADLWLGLAAAYAALGDPDRQIATLEVATREVPRRRDVRLQLADVYR
                     DNKNPAAAARVLEELTAEPHPDDGPLLDRLLTRYLWVGDYERQLVVLRKLLALEPTDL
                     AVVTDLASVARSLNRQEEALTVLEAYVRRRPGDAEGRRWLAQVYDSVGQGTRALEHWK
                     VLARLAPGDIEARDRLLPAPGGAPREEELALLERRREAEPRDEAVRQRLVALYRAAGD
                     GPRAITVQRELAALRPRDPEVLVGLGRLLVEQDRGPDAIPLYERALEIAPDRVDDALA
                     LAQLYEWADDPRRALALLERVARARPADRALQERVAALARGTDDSPAALAALDRLAAA
                     RPEGPYAQQAIDALVGANRLPEAIARQRRIAEQAPTAPGPALRLAELYGWNGQEREAI
                     AVYETLDRAGTLPEASLARLAELYRFQGRSADLLRVADRLLARRPDDAGLRDAASEAA
                     EALGRPADALRLLQPLAERRPLDEGFVLRYLTLAVQSGRPDEGLRVHRRFAASAGAPY
                     RVKAGRLLTDLGRNQDAIAEYEAALAGGLGEGDEAVRARIALAQLYDGSGAGEPALRQ
                     WEGLMRTRPRDPQVLREVGRRSLAASRNDVALRAYRALLDVQPDDAEALKRAGQLMAW
                     SNDGRGARAALERFNRVKGGDYEVHYLLGELYTAEHDEERARAEYERALRLLPARPR"
     gene            complement(9667..>10675)
                     /locus_tag="VNU03_03935"
     CDS             complement(9667..>10675)
                     /locus_tag="VNU03_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73632.1"
                     /translation="LDWEKRTRSFAPAGVPYSVETETAGAIRYMEERLLPPGKRVRLF
                     QWSGSTRVTEEAIAVLDRLGVPNINGGDTMRDREWPSYTRVAPLMRQVGRHWQTYTSA
                     SNENLYTHLWTGPFYGFRHVIETYENTERPRRVAPVNVYYHYYSGERVASLAALQYVH
                     EWVLRQPLARIFTSEYLAIVAGFRTARIARVPEGWRISGHGALSTVRFDDTRAHLDLD
                     RSSGVLGYLHHQGALYVHLAGPGPALIALRDRPPATAYLVQASHRVTAWKREAGRVAL
                     TLSGLGEKVVELAGFAGDREVAVEIREPAGSRRVRARSGADGHLTVRAGEANDVEVRV
                     G"
BASE COUNT         1476 a         3958 c         3987 g         1254 t
ORIGIN      
        1 gaccgccacc gagaggacct ggagcagcgg atagacgatc gcggccgcga gcagggtcag
       61 gagggccgcc accagcgcgg cttccccgcc cgagcgcggc cccatcaggc ggacgcgggg
      121 tggctcgact cgacggccgg cgcctcgggc accatgggct gggcggcgaa ggtcagatac
      181 tcgagatccg cgagcgtctg ctcgaggcgc cggtacgccc acgcgcagcc gagcagggcg
      241 gcgagcggat agccgaggcc gtacgcgcgc ggcccgacga cgagggtggc gacggtgagg
      301 aggagattgg ccgcgaggaa gaccccggcc acctcggcgg cggcgcggcg gcggtcgaag
      361 tagaggagca cgatgccccc gaacaggcag agcacgtgca gcaccgcgcc gacgagaccg
      421 cagcgcagga tcgacacctg caggcggtcc agcccgaggc cgtccgcgat ccacggagcg
      481 agcgccatca gcaggagcgt caccggggcc tgggccacca gcagccgtcg caggctgtcg
      541 cgcaggcacg cggtcatcgc ccgcttggcc tcgagcacct ggcggagggt gccgtgcttg
      601 gtcgcgacgc cgtagtactc gcgatagcga tcgtagaagt ccgtctccac gctgaccagg
      661 aagatcgcga gcgccggcac caccgaaagg tagcacagga agatcgggct gtcgtacagc
      721 gaggcgcgaa accagcccac gatggggtgc ccggtgggcc cgatccagaa caccacgcgg
      781 tcgaccgcga tcgccgcgtt gtagaggagg ccggccgcga tcagctccgg atagtggacg
      841 aacgcgcggg tgagatcgac cgactccggc acgcgcacca ggctgaactc gcgctccacg
      901 cgggcgcaga gcacgaagaa gatgccggcc tgcccgacca ggaacccggc caggtatccg
      961 ctcgtgccga ggtatcgccc gagggcgagc gccccgagga ggctcaccgc gttgcccagg
     1021 aagaacgcgg tcaccacgct cgcgtagtcc tgggccgcgc cgaggtagat catcaccatc
     1081 caggtgcacg agacgaccac cagcagcatc accccgaaga ggcgcagcgc gggctcgagg
     1141 ccgggcgcga gcgcgtagac gagcgtgccc agcgccaggt gggcgagcgc ggtcgcggcg
     1201 atggtcacgc ggtaggagac caccagcgcc ccgaggcggc ccacgtacag ctcgtcggcg
     1261 agctggcgcg tcaccaccat gtgggcggcc ccggtggtga cgagcgagaa cgtgtaggtg
     1321 tagacgacga tgacctggaa gcgcgcgtgg gcgagatcgc cgatcaggtc gcgcgagagg
     1381 agggccagcg acccgagcgt gcagatggag agcagccagg gccccgccga gaggaccgct
     1441 ccgtagaggt gggcgcggag ccacccggtc ccgccctcct cgacgatgag ccgcttgagg
     1501 cggaacccga tgccggccac cggcgccgct cgctcaggcc gcgcccagga gatcggcgta
     1561 gatctcccgg tagcgacgga tcaggtcgga ctcctggtag tgcgcccgca cgcgcgcccg
     1621 cgcggctcgg cccatctcct cgcagagccc ggggtcgcgg gccaggctca ggatggcccg
     1681 cgcggtggcg gccgggtcgg cgaggccggt gacgagcccg ctcgccccga gcgcccggtc
     1741 cgcgggcagc cggccctcga gcagctcgcg gcaggccccc acgtcggtgg agaccaccgg
     1801 gatgcccgcg gcagccgcct ccaggatcac gagcggcagg ccctccgaga tgctggtgag
     1861 cacaaggacg tcaacccgcg ggtagatctg cttcacgtcg acgcgcccgg tgaactccac
     1921 ccgctcgctg attcccaggg tggcggcgag ggcctggcac tccgcgaggt actccgggtc
     1981 ctcgtcgccc gggcccgcga tgaccgcgct cgcccccggg tgcgcggtga cgacgatctt
     2041 caccgcgcgg atgaacgtct tcacgtcctt gataggcacc acccgcccga cgaagccgat
     2101 gcggaactcg cccgcggccg gctccacccg cggcagcgcc gagagcggcc cgacgtcgag
     2161 cccgttgggg accaccaggg tcttggccgg gtcggcgccg tcgcggatct gggcctggcg
     2221 gttgccctcg aagatcgtca cgatgcgatc ggcctggacg taggtgatcc gcgagaggcg
     2281 ctcgaagagg cgcgcccaca gctccttgaa gaagcccggc ccggccgaga gcgtcatggg
     2341 cgcctgccgc tgcacgtaga tccactccgc ctggtcgatc tccatccggc gctcgcgcga
     2401 gtagatgccg tgctcggtca gcagcatcgg ccggccgcgg cgcacgcgct gcaccgcggc
     2461 cacgaacccg gcccagccgg tggacacggt gtgatagacc gccgcctccg gcaccggggc
     2521 gtgcagcagg cggaacatcg gcaggtgggt gaagcgccag gtccagaagt agtcgaggaa
     2581 cgagatgtcg ggggcgcgcg actcgtagag ccgtcgcacg atctcccacg agggcttgcc
     2641 gtagatgaag tcgtgcaccg acggccccgc ccccgcgtgc ggggagatct ggcgcaggag
     2701 cccttcgaga ccgtcggcgc gcccgtcctt catctcctcg tggtagcgcc agatcgtctc
     2761 ccacgccgcc gcctccgtca cctcgccctt gagcgccggc tcgtcgccgc cgtggatgcc
     2821 cacgtcgacc aggctcacca cgttgggcgg catcttgtag cgcggctcgt gggcctcccc
     2881 ctgggtggcc gagatgtgga agatcgcgaa gcgcagctcg gggagcgcgt tgaccagctg
     2941 gtggatccag gacgagaccc cgccggtcac gtaggggtag gtgccctcga ggatgaggca
     3001 gatgtcggcc gggcgctcgc tcacgccgcg ggctcccccg cccaccagcg cagcagctcg
     3061 tcggcgcgcc ccgcggccac cgcgtcgcgc gagacctcct gcagctcgcg ccagcgaccc
     3121 tggcggaaca ggatctcgga gaggagcagg tcgatctcgg tggacggcgc cgccgcgcgg
     3181 gcctccaccg cgaggtgctc cgcctcgtcg atccggccca gcgcgaggag cacccgcgcg
     3241 agcgcccggg cgggcggcgg ccagtcggga cggcgcgcgc gcgcggcgcg ataggcggcc
     3301 tccgcctcgc cgagccagtg acggcccatc gccgcgtcca gcagcccgct ctcctggtag
     3361 gcgaggcacg cgtcccccat cgccatcgcc cgctccgccg actcggggtc caggcgccgc
     3421 tgctcctgcg cctggccgat tcgctgcgcg agccgctcct cgacccgctg gagcgcgccg
     3481 gaggccgcga tctgggtatc gcggtcgggc gcctgcagcg cctcgcgcag cagctccacc
     3541 gcggcgccgt cgccgcgctt ggccaggagc ccgatgcccc accgctgcat gcgcgggtcg
     3601 gcggcgcgga tcaggtcggc caccggctgc accgagacct gcgcctgcag ccactcgaag
     3661 acgcggtcga ccggctcggg cgcctgcccc gggccgggca ggcccgccat ctccgagtgc
     3721 gcgcccgcgt agaggccgga cggtggggcc cggcgctgcc agacgcggat ggcgccgagc
     3781 ccgaccaggc cgagcaccgg cagcgccagc accagcgcca ccaccaggag ccgctcgtcg
     3841 cgccgcccgc tctcgaggcc ccgggcggcg agccacgcgg cggccacgtg ggcgagcagc
     3901 gcgagcggcc accatgaggc gagcgcggcc cccaccgcca ccagctccgc gagcccggcc
     3961 agggcccggg tcacgaggtc accccgccgg gccgctcgcg caccgcgagc acgcgggcgg
     4021 agaactcgag atcctgctcg tcgtcggcgt agggggcgaa gtggaagttc cggatctcgc
     4081 tcgagaggcg gtcgacgacc acctccgcgc gcgtgggcgc ggtctcgggc aggatgatcg
     4141 cgaaggcgtc ctcggtggcg tagcggcaca cgatgtcgac gtcgcgcagc gtgcgcgaga
     4201 acacgagcga cagcacgcgc cccaggtcgg cgcggcggtc cgccgccacc gccccgaagc
     4261 ggtggatgcg aaagatcacc accgcgagcg ggcggccgta gcggtcggcc cgcacctcct
     4321 cctcgtggat gcgccgctgg tagtaggcgt aggtgtaggc gcgcaccagc tcgtcctcga
     4381 tggtccgcgc gagggtctgc tcgtgggtgc gcgcgttctc gagggtgcgc gccccccagt
     4441 ccgcgacgag gccgaggcga tcgagcgaca ccggggtgag ccggaggaac gggagcttgg
     4501 tgaccgtgat gcagccgagc agggcgccct cggcggaggt catcggggcc gccatcagca
     4561 gctccgcgcc ctgcaggtcc tcgagcgagc cgatcgtggt gtagtctcgc acgctgaccg
     4621 gacggcccag gcggaccgcg aggcccacga gcccgtcgtc gggggcgatc tcggcgcgct
     4681 ccggcgcggg cccctcggcg gcgcgcaggc gcaggcggtc gccgtcgagc acgtagcact
     4741 ggcaggcgtc ggcctggagg aaccgccggg cggtggccgc gatcgccggg tagagccgct
     4801 ccgcgtcgag ggtctcgagc gcgcgcgccg cggtgtagag gctcgacagc gacgcgcgct
     4861 cgtcggccac ccggcgctcc agctcgtgct tggcctcggt cgcggccatg aatcgcaccg
     4921 cctggtcggc cagctcctgc tcgagcccct ccacctgcgc ctcgagggcc cgcgtcctcc
     4981 ggagctgcgt ctcgcggagc gcgccgagtc cgaagccggt cccgatcagc agcagcggag
     5041 tcacgtagcg gggcaggctc cggagctcgc tccagctcgc gtggtccatg cggaccgcca
     5101 cgcacgcggc gaccgctgcc gcggtgacga ggccgctcac cagccccgcg cgcaggccgt
     5161 gccggcccgc gagcaccacg accagcacga gggccggatt cggctgcacg tcgaggaagc
     5221 ccgggtctcg cgggcgcaac accgcgttga cgacgacgag caggagcagg cctccgccca
     5281 gctcgagcag gtagggttgg gcgcggcgaa gggcggcgag cacccggcgc ttaccgcgct
     5341 cgggggtcgc ccagggcggc ggtcaggtgg accgccatgt cgtgggccac ccgctgggcg
     5401 acacgcgcga ggccgtcctc gcgcagccac gaggagccca ggcgggcgtg actcgacgtc
     5461 cacaccacct cgccggacgc gacgtcgacg agtcgcgcgg tcacgccgac ggcgggcgtc
     5521 tcggagagac cgggcttgta acggtactcg gtcaccgagc cctgcaggac gtggctcacc
     5581 tggaggaggc ggccgacccg ctgggccgac ggcaggcggc ccgcgtcggc tggatcgagg
     5641 ttctcgcgcc ggaggagatc ggcgacctcg ctcggatcca tcaccgtcat cgccccggtg
     5701 gccaggatct cggagaccag gaggtcactg gccacctgcc ccgcgcgcag cgaggaggtc
     5761 tggttctcga gcgggaggac cgcgacgcgc gtgacccggg ccgcgccggg cttgacccag
     5821 tcctgcacgg tgctcgcgca cccgatcagc gtgagcacgc cgatcgccag cacgaccacc
     5881 cgcgaacgcc gccacatagg gtctctcagt acaggatgtc gaggcgcccg aggaacgatt
     5941 cttcctggcc cccgacggtg gtggactcgg tgttgcgagt atagagcacg cgtccttcca
     6001 gccaggggcg aattcgccat cggccctcgc cggtcacctc ccacgacgtg agtgaccggt
     6061 gcagatcgcg ccgcccgacc gagcccacct gcaggcggag cgcgggggtg acgtcccagc
     6121 cgacgagcgc cccgatcgcc acgatgtcct cccgacggag gaacgtgacc gcgtcccgca
     6181 aagggctgcc cgcggcgggc ttgtactcgg aaaaaaagac ctgggggatc agggtcacct
     6241 gcacccggct ccgcaccagc tcgatctcgg cccgcgccgc cgcctcccag cgattgccca
     6301 gatcctcgtc ggagatccgg tagtggcgga aatcgtagtg ggcggccagc accaccggca
     6361 cgaggacgcg gcgcgccacg tccacggtca cccggtcggt cgtgccgctg cgcggcaccg
     6421 ccgccaccgg attcgtgagc agctcgcgga ccgcgacctc gagggtggcg agggtggccc
     6481 gctggtcgtc gtagcccgcg ccgaggcgtc cggtggtgcg gaagatgtcc tcgcgccgct
     6541 ccgactcctc gagcccgccc cagagcgaga cggcgcgggt gggctggaac ccgaccgtcg
     6601 cgagcgcggt ccgcacctcc tcggtgaagg agggctggcc gatcacgcgc tcctggtgat
     6661 agctgccgta gcgcgtgccc gcgcggagcc accaccgatc ggagagccac ccgcgccacg
     6721 cggcctcctc cacgtgatgg gtggccgcct gctgcaggag cgagtagtgg gtcagctcga
     6781 tccgcggcgc gtggccgagg aggatgtcgc gataggccgc gagcgcgtcg cggttctcgg
     6841 gatcgccgtc gagcagccgc ccgtacaggt cgagcgcgcg gttccagcgt cccgcggcca
     6901 gctccgcggc ggcgagatcg gcggtgagcc cggtgtcccg gggctgctcg cgcgcgagcg
     6961 cgtcgagacg ccggcccgcc gccgcggggg cgccggccgc gaggtcgatc cgcgcgcgca
     7021 ggcggcgcag gcgcgcggag gtcgggtcct ccgccaggta ccggtcgacg accggggccg
     7081 cctggtcgag caggcccgcg tccaccatga cctccgcgta gtcctcgcgc aggcggcgat
     7141 cgtcggggta acgcgcgagc agcgcgcgat aggtgccgag cgcttcctcc acgagcccga
     7201 gccgcgcctg gatgcgtgcc cgcagcgtct cggcctcggg cgcgtcgatc gtgggcggct
     7261 ccgtggccgg agccgacggg gtctccgacg caccgggcgc cggggcctgg gcccgccccg
     7321 gggcgggcgc gagagcgagc aggcccgcga tcagcacgga cgcgaggcgg agcgagcgga
     7381 gccgggccat ccggcgagta tggcggggcc gtcggcccgg ttcaagacgg accgcggcgc
     7441 cggcttccgg tcagcggggg cgggcgggca gcaggcggag cgcccgctcg tactcggccc
     7501 gggcgcgctc ctcgtcgtgc tccgcggtgt agagctcgcc gaggaggtag tgcacctcgt
     7561 agtcgccccc cttcacgcgg ttgaagcgct cgagcgccgc tcgagcgcca cgaccgtcgt
     7621 tgctccaggc catcagctga ccggcgcgct tgagcgcctc cgcgtcgtcc ggctgcacgt
     7681 cgagcagcgc ccggtaggca cgcagggcca cgtcgttgcg cgaggccgcg aggctgcggc
     7741 ggccgacctc gcgcagcacc tgcggatcgc gcgggcgggt ccgcatcagc ccctcccact
     7801 ggcgcagcgc gggctcaccc gcgccgcttc cgtcgtagag ctgggccagc gcgatccgcg
     7861 cccgcaccgc ctcgtcgccc tcacccagcc cccccgcgag cgcggcctcg tactcggcga
     7921 tggcgtcctg gttccggccg agatcggtga ggagccggcc cgccttgacg cgatagggcg
     7981 cgccggcgga cgcggcgaac cggcgatgga cgcgcaggcc ctcgtcgggc cggcccgact
     8041 gcaccgcgag cgtgagatag cgcaggacga agccctcgtc gagcggccgg cgctccgcga
     8101 gcggctgcag cagccggagc gcgtcggccg gccgccccag cgcctcggcc gcctcgctcg
     8161 cggcgtcgcg cagcccggcg tcgtccggcc gccgcgcgag cagccgatcg gccacccgca
     8221 gcagatcggc cgagcgcccc tggaagcggt agagctcggc gaggcgggcg agcgaggctt
     8281 ccggcagcgt tcccgcccga tcgagcgtct cgtacaccgc gatggcctcg cgctcctggc
     8341 cgttccaccc gtagagctcg gccaagcgga gggcgggacc cggcgcggtc ggcgcctgct
     8401 ccgcgatgcg ccgctggcgc gcgatcgcct ccggcaggcg gttggcgccc acgagggcgt
     8461 cgatggcctg ctgcgcgtac ggcccctccg gccgcgccgc ggcgagccga tcgagggcgg
     8521 ccagggccgc cgggctgtcg tcggtgcccc gcgcgagcgc ggccacgcgc tcctggagcg
     8581 cccggtcggc cgggcgcgcg cgggccaccc gctcgaggag ggccagcgcg cgccggggat
     8641 cgtcggccca ctcgtagagc tgggccaggg ccagcgcgtc gtccacgcgg tcgggggcga
     8701 tctcgagggc ccgctcgtag agcgggatgg cgtccggccc gcgatcctgc tcgaccagca
     8761 gccgacccag gccgaccagg acctcgggat cgcgcgggcg gagcgcggcc agctcgcgct
     8821 ggacggtgat cgcgcgcggg ccgtcgcccg cggcccggta gagcgccacc aggcgctgtc
     8881 gcaccgcctc gtcgcgcggc tccgcctcgc ggcggcgctc cagcagggcg agctcttcct
     8941 cgcgcggagc gccacccggc gccggaagca gccggtcgcg ggcctcgatg tcaccggggg
     9001 cgaggcgcgc cagcactttc cagtgctcga gcgcgcgcgt cccctgcccg accgagtcgt
     9061 agacctgggc gagccagcgc cggccctcgg cgtcgccggg ccgccgcctg acgtacgcct
     9121 cgagcacggt cagcgcctcc tcctgccggt tcaggctgcg ggccaccgac gcgaggtccg
     9181 tcacgaccgc gagatcggta ggctcgagcg cgagtagctt gcgtagcacg acgagctgcc
     9241 gctcgtagtc gcccacccag aggtagcggg tgagcaggcg gtcgaggagc gggccgtcgt
     9301 ccggatgcgg ctcggcggtg agctcctcca ggacgcgcgc cgcggcggcc gggttcttgt
     9361 tgtcccggta gacgtcggcc agctgcaggc ggacgtcccg gcgtcggggc acctcccgcg
     9421 tcgccacctc cagagtcgcg atctgtcgat ccggatcgcc gagcgccgcg taggccgcgg
     9481 ccagaccgag ccagaggtcc gcgtcgcccc ggtcgccgtc gagcgcccgg gcgaacatcc
     9541 gctcggccag cgcgtactgc cggtcggcca ggtaggccca cccgaggtcc gcgccccacg
     9601 gcaccgacag cgcgaggagt ccgagggtga cgaccaccgc gagcgtgagc ttccagcgct
     9661 cgatgctcac ccgacccgca cctccacgtc gttcgcctcg cccgcgcgca ccgtgaggtg
     9721 tccgtccgcc ccgctgcgcg cgcgcacccg ccgggatccc gccggctcgc ggatctccac
     9781 cgcgacctcg cgatcccccg cgaatccggc cagctcgacg accttctcgc cgagccccga
     9841 gagcgtcagc gccacgcggc ccgcctcgcg cttccaggcg gtgacccggt ggctggcctg
     9901 caccaggtaa gcggtcgcgg gcggccggtc gcgcagcgcg atgagcgcgg ggccggggcc
     9961 ggccaggtgc acgtagagcg cgccctgatg gtggagatag ccgagcacgc ccgacgagcg
    10021 gtcgaggtcg aggtgggcgc gggtgtcgtc gaagcgtacg gtggagagcg cgccgtggcc
    10081 ggagatccgc cagccctcgg ggacgcgcgc gatccgcgcg gtgcgaaaac ccgcgacgat
    10141 cgcgaggtac tcgctcgtga agatgcgggc cagcggctgg cgcagcaccc actcgtggac
    10201 gtactggagc gcggcgagcg acgccacgcg ctcgcccgag tagtagtggt agtagacgtt
    10261 caccggcgcg acccgccgcg ggcgctcggt gttctcgtac gtctcgatca cgtggcggaa
    10321 gccgtagaat gggccggtcc acaggtgggt gtagaggttc tcgttcgagg ccgaggtgta
    10381 ggtctgccag tgccggccca cctgccgcat caggggcgcg acgcgggtgt agctcggcca
    10441 ctcgcggtcc cgcatggtgt ccccgccgtt gatgttcggc accccgaggc ggtcgaggac
    10501 cgcgatcgcc tcctcggtca cccgcgtgga cccggaccac tggaagagcc gcacccgctt
    10561 gcccggcggc agcagccgct cctccatgta ccggatggcc cccgcggtct cggtctccac
    10621 cgagtacggc acccccgccg gtgcgaagct gcgcgtgcgc ttctcccagt cgagc
//
LOCUS       DATMCV010000166         2884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_274826, whole genome shotgun sequence.
ACCESSION   DATMCV010000166 DATMCV010000000
VERSION     DATMCV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274826"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VNU03_03940"
     CDS             complement(<1..162)
                     /locus_tag="VNU03_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWP73633.1"
                     /translation="MRHATSPVKKPRASPVGQLIKQWRERRRLSQLSLAVDAEISTRH
                     LSFIETGRSQ"
     gene            275..676
                     /locus_tag="VNU03_03945"
     CDS             275..676
                     /locus_tag="VNU03_03945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWP73634.1"
                     /translation="MAIDPSHFNDRMQGTLSTLLGLRIVEAGPDRVVAELTIRDELRT
                     VGGALHGGTLMALADTVGAAATVVNLPAGASTTTLESKTNFFAAGRSGVVRAEATPLH
                     KGKRTQVWQTRVTDESGRLLSLTTQTQMVLM"
     gene            673..1635
                     /locus_tag="VNU03_03950"
     CDS             673..1635
                     /locus_tag="VNU03_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HWP73635.1"
                     /translation="MKVAFAGVFAGQIADRVRARVRVLCEVVQGGEQEVLARLGDVDV
                     LVSMAFTREMATAAPHLRLVQVPGAGLDRVDRGALGPETALANAYGHDVGIAEYVMGA
                     MLALARNFCRIDASLRQGRWEGVWSGTPVPLWSELAGKTLGIVGYGRIGQAVARRALA
                     FDMEVMAIRRDASRPDPHGLAYLRGPEALDDVLARADVLALTLTLTPDTRGLIDARAL
                     ALMMPTAVLINVARGEVVDEDALYEALSGGALAGAALDVWYRYPQGSTPTLPGHRPFH
                     TLPNVLMTPHVSGWTEGMMEARATVIAENIHRVARGEAPVNLIR"
     gene            1905..2846
                     /locus_tag="VNU03_03955"
     CDS             1905..2846
                     /locus_tag="VNU03_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HWP73636.1"
                     /translation="MTPTIDRERIERTHSLIRPHIRLTPVMELTGADFGLPAFPLTLK
                     LELFQHAGSFKTRGAFANLLTRSIPAAGVVAASGGNHGAAVAYAAMQLGVPAHIFVPT
                     VSSPAKISRIRGYGADLVVVGDRYADALAASQAFAARSGALPVHAFDQEETLLGQGTI
                     GLELARQAPDLDTLLVAVGGGGLIGGIAAWCAGKPRVIGVEPESAPTLTQALAAGKPV
                     DAEAGGIAADSLAPQRVGELMFPIARAHIARVVLVPDAAIRRAQEALWDGLRLAAEPG
                     GAAAFSALLGGGYVPAPGERVGVLVSGGNTTAVDFGR"
BASE COUNT          375 a         1022 c         1085 g          402 t
ORIGIN      
        1 ctgggagcgg ccggtctcga tgaagctcag gtggcgggtg gagatctcgg cgtcgacggc
       61 caggctcagc tggctcagcc gccgccgctc ccgccactgc ttgatcagct ggcccaccgg
      121 gctcgcccgg ggcttcttga cgggggaggt ggcgtgcctc atggcgggga aggtacaccg
      181 gcgcctgccg ctccgccatt accttcgagg taattgaact gctgcgccgg acgggccagg
      241 atagccgggg cacgaggccc ggggaggaac atccatggcg atcgatccga gtcacttcaa
      301 cgaccgcatg cagggcaccc tcagcaccct cctgggattg cgcatcgtgg aggcgggccc
      361 ggaccgggtg gtggccgagc tcaccatccg cgacgagctg cgcaccgtcg gcggggcgct
      421 gcacgggggc accctgatgg cgctggccga cacggtgggc gcggcggcca ccgtcgtcaa
      481 cctgcccgcg ggcgcgtcca cgaccacgct cgagtccaag accaacttct tcgcggccgg
      541 ccggagcgga gtggtgcggg ccgaggccac gccgctccac aagggcaagc ggacccaggt
      601 gtggcagacg cgggtcaccg acgagtcggg gcggctgctc tcgctgacca cgcagacgca
      661 gatggtgctg atgtgaaggt cgccttcgcc ggcgtcttcg cgggacagat cgcggaccgg
      721 gtgcgcgccc gggtgcgggt tctgtgcgag gtcgtgcagg gcggcgagca ggaggtgctc
      781 gcgcggctcg gcgacgtgga cgtgctggtg agcatggcct tcacgcgcga gatggccacc
      841 gcggccccgc acctgcggct ggtgcaggtg cccggcgccg gcctcgaccg cgtggatcgc
      901 ggggcgctcg gaccggagac ggcgctggcc aatgcctacg ggcacgacgt cggcatcgcc
      961 gagtacgtca tgggcgccat gctcgcgctc gcccggaatt tctgccggat cgacgcgagc
     1021 ctgcgccagg gccgttggga gggcgtgtgg tccggcacgc cggttccgct ctggtccgag
     1081 ctggccggca agaccctcgg catcgtgggc tacgggcgga tcggccaggc ggtggcgcgt
     1141 cgcgcgctgg ccttcgacat ggaggtcatg gccatccgcc gcgacgccag ccggcccgac
     1201 ccgcacggcc tggcgtacct gcgcgggccg gaggcgctcg acgacgtgct ggcgcgcgcc
     1261 gacgtcctgg ctctcacgct gaccctcacg ccggacacgc ggggcctgat cgacgcccgc
     1321 gcgctcgccc tcatgatgcc caccgcggtg ctcatcaacg tggcgcgcgg cgaggtggtg
     1381 gacgaggacg cgctctacga ggcgctgtcc gggggcgccc tcgcgggcgc cgcgctcgac
     1441 gtgtggtacc gctatccgca ggggagcacg ccgaccctgc ccggccatcg cccgttccac
     1501 acgctgccga acgtcctgat gaccccgcac gtatcgggct ggaccgaggg catgatggag
     1561 gcgcgcgcca cggtcatcgc ggagaacatc caccgggtgg cccggggcga ggcgccggtc
     1621 aatctcatcc gctgaggcgg agcaggtgcc taagcgcggc agccgcattg ccacggccgg
     1681 gtaaggggag ccgccagaat gcgatctaag tacccgtcag tcttgagggg ctcccttggc
     1741 attccgcttg cgatccttcc cggccatggc cagtcgccag gcgtcgcagc agatccgcgg
     1801 ggcgtgccgc tgcgggcaca tgatgagcat gcactgccac gtcgacggct ccgggccgtg
     1861 tgccgagccg ggctgcgcct gcgagggtgc taccatgatg gccggtgacg cccacgatcg
     1921 accgggagag gatcgagcgg actcatagcc tgatccgccc ccacatccgc ctcacgccgg
     1981 tgatggagct gaccggcgcc gacttcggcc tccccgcctt ccccctcacg ctcaagctcg
     2041 agctgttcca gcacgcgggc tccttcaaga cgcggggtgc cttcgccaat ctgctgacgc
     2101 gctccatccc ggccgcgggt gtggtcgcgg cctcgggcgg caatcacggg gcggcggtcg
     2161 cctacgcggc gatgcagctt ggcgtcccgg cgcacatctt cgtgcccacc gtgtcctcgc
     2221 cggccaagat cagccgcatc cgcggctacg gcgccgacct cgtggtggtc ggcgaccgct
     2281 acgccgacgc gctggccgcg agccaggcct tcgcggcgcg ctccggcgcc ctgccggtgc
     2341 atgccttcga ccaggaggag accctcctcg gccaggggac gatcgggctc gagctggcgc
     2401 gccaggcccc ggacctcgac accctgctgg tcgcggtggg cggcggcggc ctcatcggcg
     2461 gcatcgcggc ctggtgcgcg gggaagccgc gcgtgatcgg ggtcgagccc gagagcgcgc
     2521 ccacgctgac gcaggcgctc gccgcgggca agccggtgga tgccgaggcg ggcgggatcg
     2581 cggccgactc gctcgccccg cagcgggtgg gcgagctgat gttcccgatc gcgcgggcgc
     2641 acatcgcgcg ggtggtgctc gtgcccgacg cggccatccg gcgggcccag gaggcgctct
     2701 gggacggcct gcgcctggcc gcggagcccg gcggggcggc cgccttctcc gcgctgctcg
     2761 gtggcggcta cgtgccggct cccggcgagc gggttggcgt cctggtgagc ggaggcaaca
     2821 cgactgcggt agacttcggg cgatgagcaa aggcgacctc gcgacggccc tcgagcatct
     2881 ggcg
//
LOCUS       DATMCV010000167         6936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_874844, whole genome shotgun sequence.
ACCESSION   DATMCV010000167 DATMCV010000000
VERSION     DATMCV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874844"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VNU03_03960"
     CDS             <1..372
                     /locus_tag="VNU03_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWP73637.1"
                     /translation="AAAGLATLAELRRPGTYERLFATGTRLKQGLEAAARRAGLPAQV
                     AGEAPVFEIYFTDRPITDYRATLTADRDRHAAFTREMLVRGVVKAAQKFYVSLVHTDT
                     EVARTLQVFEESLAAVAGTSH"
     gene            408..1700
                     /locus_tag="VNU03_03965"
     CDS             408..1700
                     /locus_tag="VNU03_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73638.1"
                     /translation="MANHGNEQEFWYRGKITRRRWLGLSAAAAGALGSGMLVPAPWRD
                     AFGQAKPYKIGSLQPLSGAAAAGGKTALVGSQMAVDRINKSGGVNGRPIELIVGDYES
                     KPDVGQRKAEKLVVEDKIDAHQGGFLSNVCIACLPVWEEHKIVNMIGVCLDTTITTSK
                     CSRYSFRPFDYAPAQAVAFAPYLVSKLGKKWHIGYADYSWGQSTRDAYAEQIKKAGGE
                     VVGTTGIPLGTADMTAFLSKITGSFDGFFGIFFGKDGVTIGNQAFDLGLTKRYKWAGD
                     GAIAESTNLPALGNKIEGFVGINRYIPVLEGPLNTPAHKKFFDEAVTRLKQIDPSGPL
                     PDRYVQSNFEAMNFLKLGIQKSGFKGREDTMKLIEALEGLEVKAGDDFPQGDKTLRKD
                     DHQAFLSEFIFDIKGGKHHILEVVPKEKTMFPPACKFA"
     gene            1739..2500
                     /locus_tag="VNU03_03970"
     CDS             1739..2500
                     /locus_tag="VNU03_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73639.1"
                     /translation="MVSARGGAGSPILRTEGLTVRFGGLTALNDVNVEVRRGEIRAII
                     GPNGAGKSTFFNCLTGVLHPTAGRILLDGEDVTGLPPNRISQKGIARSYQITNIMPNA
                     TTLENVRIAAQSRRHSWSLLTHHRAYRDILSRAEGALAAVGLAGKADELAANLSHGEQ
                     RNLEIGIALATEPQLLCLDEPTAGMSAAETHDTMELVRRIAKDLTILIVEHDMQVVME
                     LCHRITVLHYGEVLAEGTPAEIQGNPKVMEVYLKT"
     gene            2497..3234
                     /locus_tag="VNU03_03975"
     CDS             2497..3234
                     /locus_tag="VNU03_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73640.1"
                     /translation="MSGAASPASPLVAVEGIHTFYGKSHILHGVSLEVGRGEVVGLLG
                     RNGVGKSTTLKTVMGLVRPSQGEVRYEGRAITGLAPHRLARLGIGYVPEDRRIFRLLT
                     VMENLRTGLDRAGVDEGRKQALLEKVYDYFPVLADRRNQAGGTLSGGEQQMLAIARAM
                     MLEPKIILLDEPTEGLMPRMVSQIRQIIDALHREGVAILLVEQNVPLTLEACARIYLM
                     EKGQVRHQAAAAELRADAAVIHQYMGI"
     gene            3238..4110
                     /locus_tag="VNU03_03980"
     CDS             3238..4110
                     /locus_tag="VNU03_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73641.1"
                     /translation="MPILLDQILIQTINGIVTGMILALVASGLTLIFGIMDVVNFAHG
                     ELFMLGAYVGVTTMVATGNFWIALLVATVVIALLGAVIQITTLRPLLGRDPLTTILAT
                     FGLSLVLQNYALWQFGPVARKIQEPFTGHFSLFYLDYPWYRLVIAGLSAAIIGALWLF
                     LKFGTYGIWIRATTQDRVMASAMGIPVPWVHTAVFAIGAGMAAASGVLFGPLVGVNHA
                     MGLDWVLKAFIVVVVGGMGNLGGSIAAAIFVSLLEAYASLWVSPAQAVIVSFVALILT
                     LLFRPTGLFVPTPK"
     gene            4107..5063
                     /locus_tag="VNU03_03985"
     CDS             4107..5063
                     /locus_tag="VNU03_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73642.1"
                     /translation="MISQRAPLAFWTGFGVVLALLILAPLVLPEFWRRFVTEILIWGL
                     LAMSSDILIGYTGMISFGHSAFFGLGMYGAAAALLSTRPPNLWLAFVYGLAAAAVVAV
                     FVAYFSTRLRDIYFSITTLIFSQIFYVIIFTWTEVTGGENGLSFKRPALAIPGLFSVP
                     FDSNTLHWFVLAVVTVSYLVLRRITHSPFGMVLQSIRENEPRTRAVGYAVERYKIVAV
                     MLSGVFAGLAGVLYALQNRFAAPDYVFFVVSGEVVIFNVMGGIGTLVGPIVGAAAFLL
                     LREGFSRFFTEFYLIPVGIIFTAMVIFMPQGLLGFLRRRLNE"
     gene            complement(5187..5603)
                     /gene="dut"
                     /locus_tag="VNU03_03990"
     CDS             complement(5187..5603)
                     /gene="dut"
                     /locus_tag="VNU03_03990"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018725323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWP73643.1"
                     /translation="MVRADPSLALPAYAREGDAGLDLAAALAVTLPPAGRQLVPTGLR
                     VAIPDGYAGLVLPRSGLALRAGVTVLNAPGLIDSGYRGEIGVLLVNHGTEAVTIGRGE
                     RIAQLVIQPVARASLVEVRELGESRRGTGGFGSTGV"
     gene            5851..6045
                     /locus_tag="VNU03_03995"
     CDS             5851..6045
                     /locus_tag="VNU03_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73644.1"
                     /translation="MLERLGPSGLLRLAAVITLGASLLLSGCAASSHESPQTAADEKE
                     GWSRCDHGRPGTITLVCWKR"
     gene            6056..6916
                     /locus_tag="VNU03_04000"
     CDS             6056..6916
                     /locus_tag="VNU03_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73645.1"
                     /translation="MHARSLRPLLTLGALVLALAPAAPARGENTADLAPEIARLRARL
                     ADNAAREQHCMAGATQPVWTASLATDLKDLRDRARQAATEGSAVDAQRWRDLARKAET
                     LEAQAVVNARTGAELFQSQQIGLDCLDRFAGEREALHASLEVAVADPTAYGDSLQQAR
                     EHGTARLRQDLARLRERSRALSAHWRQTSPEAGAGATDLKAEIAGLRRRHTVVLESEA
                     ARALADPSLRAAEALVAAAAAWEQERATGNRLAAARDEAERRLVARERDEAGRLARDY
                     WGTAERLLAR"
BASE COUNT         1054 a         2485 c         2328 g         1069 t
ORIGIN      
        1 gcggcggccg gcctggccac cctggccgag ctgcgccggc ccggcaccta cgagcgcctc
       61 ttcgcgaccg gcacgcgcct caagcagggg ctcgaggcgg cggcccgccg cgcggggctc
      121 ccggcccagg tggcgggcga ggcgccggtg ttcgagatct acttcaccga ccggcccatc
      181 accgactacc gcgccaccct caccgcggac cgcgaccggc acgccgcgtt cacccgcgag
      241 atgctcgtgc ggggcgtcgt gaaggcggcc cagaagttct acgtctcgct cgtccacacc
      301 gacaccgagg tcgcccgcac gctgcaggtc ttcgaggaat cgttggccgc ggtcgcggga
      361 acttcccact gagcggctcg attacagaac aacaggaggc tctccccatg gccaatcacg
      421 gcaacgagca ggagttctgg tatcgcggca agatcacccg gcggcggtgg ctcggcctga
      481 gcgcggcggc ggccggtgcg ctcggctccg gcatgctcgt ccccgccccg tggcgggacg
      541 cgttcggcca ggccaagccc tacaagatcg gctcgctcca gcccctctcg ggcgcggcgg
      601 cggccggcgg caagaccgcg ctggtcggca gccagatggc ggtggaccgg atcaacaaat
      661 cgggcggcgt caacggccgg ccgatcgagc tgatcgtggg cgactacgag tcgaagccgg
      721 acgtgggaca gcgcaaggcg gagaagctgg tggtcgagga caagatcgac gcccaccagg
      781 gcggcttcct ctccaacgtg tgcatcgcgt gcctgccggt gtgggaagag cacaagatcg
      841 tcaacatgat cggggtgtgc ctcgacacca ccatcaccac cagcaagtgc agccgctact
      901 ccttccggcc gttcgactac gcgccagcgc aggcggtggc cttcgccccc tacctggtca
      961 gtaagctcgg caagaagtgg cacatcggct acgccgacta ctcgtggggc cagtccaccc
     1021 gcgacgccta cgccgagcag atcaagaaag cgggcggcga ggtggtgggc accaccggca
     1081 tcccgctcgg caccgcggac atgaccgcgt tcctgtccaa gatcaccggc agcttcgacg
     1141 gcttcttcgg gatcttcttc ggcaaggacg gcgtcaccat cggcaaccag gccttcgacc
     1201 tggggctgac caagcgctac aagtgggccg gcgacggcgc catcgcggag tccaccaacc
     1261 tgcccgcgct cggcaacaag atcgagggct tcgtcggcat caaccgctac atcccggtgc
     1321 tcgagggccc gctcaacacc cccgcccaca agaagttctt cgacgaggcg gtcacgcggc
     1381 tcaagcagat cgatccctcg gggccgctcc ccgatcgcta cgtgcagtcc aacttcgagg
     1441 cgatgaactt cctcaagctc ggcatccaga agtccggctt caaggggcgc gaggacacca
     1501 tgaagctgat cgaggcgctc gagggcctgg aggtcaaggc gggcgacgac ttcccccagg
     1561 gcgacaagac gctgcgcaag gacgaccacc aggcgttcct gagcgagttc atcttcgaca
     1621 tcaagggtgg caagcaccac atcctcgagg tggtgccgaa ggaaaagacg atgttcccgc
     1681 cggcctgcaa gttcgcctag tgacggcgga gcagtcgacc ggcgccgccc ccggctccct
     1741 ggtctccgcg aggggcggcg ccggctctcc gatcctgcgc accgagggcc tgaccgttcg
     1801 gttcggcggg ctcaccgcgc tcaacgacgt caacgtcgag gtccggcgcg gggagatccg
     1861 cgccatcatc gggcccaacg gcgccggcaa gagcacgttc ttcaactgcc tgaccggcgt
     1921 gctgcacccg accgccgggc gcatcctcct cgacggcgag gacgtgaccg gcctgccccc
     1981 gaaccgcatc tcgcagaagg gcatcgcgcg ctcgtaccag atcaccaaca tcatgcccaa
     2041 cgcgaccacc ctggagaacg tgcgcatcgc ggcccagtcg cggcggcaca gctggagtct
     2101 cctcacccac caccgcgcct accgcgacat cctgagccgg gccgagggcg cgctcgcggc
     2161 ggtgggcctc gcgggcaagg ccgacgagct ggccgcgaac ctctcccacg gcgagcagcg
     2221 gaacctcgag atcggcatcg cgctggccac cgagccgcag ctcctgtgcc ttgacgagcc
     2281 gaccgcgggc atgagcgcgg ccgagaccca cgacaccatg gagctggtcc gccgcatcgc
     2341 gaaggacctg accatcctga tcgtggagca cgacatgcag gtggtgatgg agctctgcca
     2401 ccggatcacg gtgctgcact acggcgaggt gctggccgag gggacgccgg ccgagatcca
     2461 gggcaacccg aaggtgatgg aggtctacct caagacgtga gcggcgccgc ctctccggcc
     2521 tcgcccctcg tcgccgtcga ggggatccac accttctacg gcaagagcca catcctccac
     2581 ggggtctccc tggaggtcgg gcgcggcgag gtcgtgggtc tcctgggccg gaacggggtc
     2641 ggcaagagca ccacgctgaa gacggtgatg ggcctggtgc gcccgagcca gggcgaggtg
     2701 cgctacgagg gccgcgccat caccggcctc gcgccccacc ggctggcgcg cctcggcatc
     2761 ggctacgtgc cggaggaccg ccgcatcttc cgcctgctca ccgtgatgga gaacctgcgc
     2821 accggcctcg accgggcggg ggtggacgag ggccgcaagc aggcgctgct cgagaaggtc
     2881 tacgactact tcccggtcct cgccgaccgc cgcaaccagg cgggcggcac gctctccggc
     2941 ggcgagcagc agatgctcgc gatcgcgcgg gcgatgatgc tcgagccgaa gatcatcctg
     3001 ctcgacgagc cgaccgaggg cctcatgccg cgcatggtct cccagatccg ccagatcatc
     3061 gacgccctcc accgggaagg ggtggcgatc ctgctggtcg agcagaacgt gccgctcacc
     3121 ctcgaggcgt gcgcgcggat ctacctcatg gagaagggcc aggtgcgcca ccaggccgcc
     3181 gccgccgagc tgcgcgccga tgccgcggtc atccaccagt acatggggat ctagggcgtg
     3241 cccatcctcc tcgaccagat cctgatccag accatcaacg ggatcgtgac cggcatgatc
     3301 ctggccctcg tcgcctccgg tctcaccctc atcttcggga tcatggacgt ggtgaacttc
     3361 gcccacggcg agctgttcat gctgggcgcg tatgtgggcg tgaccaccat ggtggcgacc
     3421 ggcaacttct ggatcgcgct gctggtagcg acggtggtga tcgccctgct cggcgcggtc
     3481 atccagatca cgaccctgcg cccgctgctc gggcgcgatc ccctcaccac gatcctggcc
     3541 accttcgggc tctcgctcgt cctgcagaac tatgccctct ggcagttcgg gccggtggcc
     3601 cggaagatcc aggagccctt caccggccac ttctcgctct tctacctgga ctacccctgg
     3661 taccgcctgg tcatcgcggg actgtcggcc gccatcatcg gggcgctctg gctcttcctc
     3721 aagttcggga cctacggcat ctggatccgc gccaccaccc aggatcgggt gatggcctcc
     3781 gcgatgggaa tcccggtccc gtgggtgcac accgcggtgt tcgcgatcgg ggccgggatg
     3841 gccgcggcca gcggggtgct cttcgggccg ctggtgggcg tcaaccacgc gatgggcctc
     3901 gactgggtgc tcaaggcctt catcgtggtg gtggtcgggg gcatgggcaa tctcggcggc
     3961 tccatcgcgg ccgcgatctt cgtcagcctg ctcgaggcct acgcctcgct ctgggtgagc
     4021 ccggcccagg cggtcatcgt gtcgttcgtg gcgctgatcc tgaccctgct cttccgcccc
     4081 accggcctct tcgtgccgac ccccaagtga tcagccagcg cgctccgctc gcgttctgga
     4141 ccggcttcgg cgtggtgctg gcgctcctga tcctggcgcc tctcgtgctc cccgagttct
     4201 ggcgccgctt cgtcaccgag atcctgatct ggggcctgct cgcgatgtcc tccgacatcc
     4261 tgatcggcta caccggcatg atctcgttcg gccactccgc gttcttcgga ctcgggatgt
     4321 acggcgcggc cgcggccctg ctctcgacgc ggcccccgaa cctgtggctc gccttcgtct
     4381 acggcctcgc cgcggcggcg gtggtggcgg tcttcgtggc ctacttctcg acccggctgc
     4441 gcgacatcta cttctcgatc acgaccctca tcttctccca gatcttctac gtgatcatct
     4501 tcacctggac cgaggtgacc ggcggggaga acgggctcag cttcaagcgg cccgcgctgg
     4561 ccatccccgg gctcttctcg gtcccgttcg attcgaacac actccactgg ttcgtgctcg
     4621 cggtggtaac cgtgtcctac ctcgtcctgc gccggatcac gcattcgccg ttcggcatgg
     4681 tgctgcagtc catccgggag aacgagcccc ggacccgcgc ggtcggctac gcggtcgagc
     4741 gctacaagat cgtggcggtg atgctctccg gggtgttcgc gggactggcc ggggtgctct
     4801 acgcgctgca gaaccgcttc gcggcgcccg actacgtctt cttcgtggtc tcgggcgagg
     4861 tcgtgatctt caacgtgatg ggcgggatcg gcaccctggt gggcccgatc gtgggcgccg
     4921 cggccttcct gctgctccgc gagggcttct cgcggttctt caccgagttc tacctgatcc
     4981 cggtgggcat catcttcacc gccatggtca tcttcatgcc tcagggcctg ctcggcttcc
     5041 tgcggcggcg gctgaacgag tagtccccac gcatctacac cctgagcccc gagtaggggt
     5101 caggtcttac attccgacat tttcccggca cgtggccgta ccgccgactt ccacgagaat
     5161 gtcggagtgt aagacctgac cccagctcag acgccggtcg agccgaagcc gccggttccg
     5221 cgtcggctct cgcccagctc gcgcacctcc accaggctcg cccgcgccac cggctggatc
     5281 acgagctgcg cgatgcgctc gccgcggccg atggtcaccg cctcggtgcc gtggttgacg
     5341 agcagcaccc cgatctcgcc gcgatacccc gagtcgatga ggccgggcgc gttcagcacg
     5401 gtcacgccgg cgcgaagcgc cagccccgat cgcggcagca ccagccccgc gtagccatcg
     5461 gggatcgcca cgcgcagccc ggtcggcacg agctggcggc cggcgggcgg cagcgtcacc
     5521 gccaacgccg ccgcgagatc gagtcccgca tcgccttctc tcgcgtaggc gggaagcgcg
     5581 aggctcggat cggcgcgcac cacttcgatg cggacaacgg gatctcttcg gtcggtcact
     5641 cgtgacgtct ccgcgaaggg attgcgcgcg ctactcgtgc gcgctcgcga agagtagcag
     5701 aaagcgaggc cggcttgaca aaattggggg cgagcgaccg agcgtatcgg gccataagta
     5761 tgcgataaaa tgctgatatt tcgctggccc cctccttgct attccagtgg atcgcggttg
     5821 caagcagatc caaacgagga ggcagcggag atgctggagc gactgggacc gtcgggcctg
     5881 ctgaggctgg ccgcggtgat cacactgggc gcctcgctcc tgctctccgg ctgcgccgcg
     5941 tccagccacg agtcgcccca gaccgcggcc gacgagaagg aaggctggag ccgctgcgat
     6001 cacggccgcc cggggacgat caccctcgtg tgctggaagc gctaggcgcc tcgtgatgca
     6061 cgcgcgctcc cttcgtcccc tgctcacgct tggcgcgctc gtgctggcgc tcgctcccgc
     6121 cgctcccgcg cgcggcgaga acacggcgga cttggccccg gagatcgcgc gcctgcgcgc
     6181 gcgcctggcc gacaacgcgg cgcgggagca gcactgcatg gccggggcga cccagccggt
     6241 gtggaccgca tcgctggcca ccgacctcaa ggatctgcgg gatcgcgccc gccaggccgc
     6301 caccgagggc tccgccgtgg acgcgcagcg ctggagagat ctggcgcgca aggcggaaac
     6361 cctcgaggcc caggcagtgg tgaacgcgcg caccggcgcc gagctgttcc agagccagca
     6421 gatcgggctc gattgcctgg atcgcttcgc gggcgagcgc gaagccctgc acgcctccct
     6481 cgaggtggcg gtggccgatc cgaccgctta cggcgactcg ctccagcagg cccgcgagca
     6541 cggcaccgct cggctccgac aggacctggc ccggctccgg gagcggagcc gggcgctctc
     6601 cgcgcactgg aggcagacct cgccggaggc cggtgcgggc gcgaccgatc tcaaggccga
     6661 gatcgccggg ctgaggcgcc gccacaccgt ggttctcgaa agcgaggccg cccgcgcgct
     6721 ggccgacccg tccctgcggg ccgccgaggc tctcgtggcc gccgcggctg cgtgggagca
     6781 ggagcgtgcg acgggaaacc ggctcgcggc ggcgcgggac gaggcggagc ggcggctggt
     6841 ggcccgggaa cgcgatgagg cgggacgtct ggcgcgtgac tactggggga ccgccgagcg
     6901 actgctcgcc cggtgaagcg ggccgcctat gcgggt
//
LOCUS       DATMCV010000168         4361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_341545, whole genome shotgun sequence.
ACCESSION   DATMCV010000168 DATMCV010000000
VERSION     DATMCV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341545"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(476..1204)
                     /locus_tag="VNU03_04005"
     CDS             complement(476..1204)
                     /locus_tag="VNU03_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HWP73646.1"
                     /translation="MHSLGKIGWGAAALALALLAVTSPAPAQFDKLLKGLSGQGSGLS
                     DTKIADGLKEALQVATGKTVSLTGKTDGYFGNQAIKILMPEKLKTLETGLRTVGYGPQ
                     VDEFVLSMNRAAESAAPQAKQIFLDAIGKMSFDDARKILNGGDTAATEFFRSKTTPQL
                     TTAFRPAVSKSMNDVGVTRQYKDLVSRFDAIPFARSQAFDLDGYVVDKGLGGLFHVLG
                     DQEKDIRTNPSARATDLLKEVFKR"
     gene            complement(1221..2195)
                     /locus_tag="VNU03_04010"
     CDS             complement(1221..2195)
                     /locus_tag="VNU03_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP73647.1"
                     /translation="MASETPRDGSAAAPADVEGQQWAIQRRLGEIFGKLGEKLAELTG
                     RVDVAVKAETSFADIAGLAEAKGLVRSFVTALTDPELYGKWGIAPPKGMLIYGPPGTG
                     KTLLARALATEAGAVFYHLRLGTLTSKFGPNTGELLQEVLGLAREQGKGVVLLDQADA
                     LALEHLLPPAQAREAGARIVAALCEKLDTLDASARLVVIAATSRTDAVDPSLVAPGRL
                     DHLVEVRLPDAAAQEQVIALTRARAEHNAGRPLVGDLDYRLLLPPMGGMSGGEIRDIV
                     RRALEEKVHAAGQGQEAGLVTTQDLLRQVDVYRRIRAVVEKIRYGQYL"
     gene            complement(2200..2733)
                     /locus_tag="VNU03_04015"
     CDS             complement(2200..2733)
                     /locus_tag="VNU03_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HWP73648.1"
                     /translation="MMQIRRLSRFVLVLGLGVMLSGARKPTEIAAPPVGGPQEISVVG
                     VYSDPQGQHGVMIEGKRDKRRVLMVIGPAEATAIALPLEHVTPPRPLTHDLFLTLFGR
                     LKVGVTRVVITDLRNNTYYATLHLTVDGAPMELDSRPSDAIALAIRAKAPVFAEDRVF
                     EKSERLPGGPAGPGERI"
     gene            complement(2796..4019)
                     /locus_tag="VNU03_04020"
     CDS             complement(2796..4019)
                     /locus_tag="VNU03_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73649.1"
                     /translation="MTGPAAEFLRHGPFARIMLTNFFFFGALNGFLLLPLYIQQLGGD
                     EADIGLVQGFYSAAGIVCQPVVGALIDYLGRRFFMRLGVGLLVVSCALFVVSSPLPLL
                     GALRVLQGVAFSAFFVANYIHVVEMVPAERRGWALGIFGLSGLVSTSLAPLFGELMIR
                     RFGFPWFFAAATVIAGGALAVCWNVRDVRPAALGARPGLHVIREGLLELRHMHMVLGF
                     FFGLGTGTIFTFLPTFGETLGVASVGLFYTAYAVAAMLVRVMGGTLIDVRGRRAVIIP
                     CMFVQAAATCIIAIVALLFWPSLRLPVLPFLFLAGFLAGAAHGWLYPAMSALLMDVTL
                     ERRRGSAVGIFSSVILLGNTTGAVIFGYVAHGLGYAIMWSALTVLLAVGSLLSTRLRV
                     GYAVPSPAVSGAARG"
     gene            4065..>4361
                     /locus_tag="VNU03_04025"
     CDS             4065..>4361
                     /locus_tag="VNU03_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73650.1"
                     /translation="MNAREYSTRSRAGRTPETVAQVMAAAARLEPDGRGRLLAPASLQ
                     SWPGIVHGGGLVALLDAAARALGAGDGPRRLEGRLTSSVPIATALDLEGHAEHDA"
BASE COUNT          674 a         1573 c         1532 g          582 t
ORIGIN      
        1 gacggtatgc gtcggcacat cgtacgagct cgtgtgtctg agggtgttgc atcatcactg
       61 tctccgtcgt ggagcagccg gctaacacga ccgccgctgc cataccggcg accagccagc
      121 aatgctgcgt gatacgggtc acatgtgtca tcccatcacc tttcttcgtg gcagcctctc
      181 tgcactgccc gggggggcac cacgcggaac gtcatccgca catttccgcc tggcgcctcc
      241 tcctcatgcg cggtcgcgtc gcggaacttt cacgtagccc cgctcggtca gaccgctgcg
      301 gaaggcgtcg aagaaaccgc cggaacacgg tgtcggtcgg ggaggacact cccaggaagc
      361 cgatgcgatg gagcttgccc gccggctggg gcggcggcga gggacgcgtc gaggaaaccg
      421 cccgccacgc tcgagatgaa gacccggcgg ccgatcacgg gcggcaggat accactcagc
      481 gcttgaagac ctccttcagg agatcggtcg cgcgcgccga cggattggtg cggatgtcct
      541 tctcctggtc gccgaggacg tggaagaggc cgccgaggcc cttgtccacc acgtagccgt
      601 ccaggtcgaa ggcctgcgag cgggcgaacg ggatcgcgtc gaagcgcgag accaggtcct
      661 tgtactggcg ggtgaccccc acgtcgttca tcgacttcga caccgccggc cggaacgcgg
      721 tggtgagctg cggggtcgtc ttcgagcgga agaactcggt cgcggcggtg tcgcccccgt
      781 tcaggatctt gcgcgcgtcg tcgaagctca tcttgccgat cgcgtcgagg aagatctgct
      841 tcgcctgcgg ggccgcgctc tcggcggccc ggttcatgct gagcacgaac tcgtcgacct
      901 gcgggccgta gcccaccgtg cgcagccccg tctcgagcgt cttgagcttc tcgggcatca
      961 ggatcttgat cgcctggttg ccgaagtagc cgtcggtctt gccggtgagg ctcaccgtct
     1021 tgccggtggc gacctgcagc gcctccttga gcccgtcggc gatcttggtg tccgagagcc
     1081 cgctgccctg cccggagagc cccttgagca gcttgtcgaa ctgcgccggc gcggggctcg
     1141 tcaccgcgag cagcgcgagc gccagggccg ccgcccccca tccgatcttc ccgagtgagt
     1201 gcatgcgctc tcctgaccgg ctagaggtac tgcccgtagc ggatcttctc caccaccgcg
     1261 cggatccgcc gatagacgtc gacctggcgc agcagatcct gggtggtgac gagccccgcc
     1321 tcctgtccct gtcccgccgc gtgcaccttc tcctccagcg cgcgccgcac gatgtcgcgg
     1381 atctccccgc cgctcatgcc gcccatcggc gggagcagga ggcgatagtc gaggtcgccc
     1441 accagcggcc ggcccgcgtt gtgctcggcc cgcgcgcggg tcagcgcgat gacctgctcc
     1501 tgggccgccg cgtccggcag gcgcacctcg accaggtggt ccaggcggcc gggcgccacc
     1561 agcgaggggt cgaccgcgtc ggtgcggctg gtggccgcga tcaccaccag gcgcgcggag
     1621 gcgtcgaggg tgtcgagctt ctcacacagc gcggccacga tgcgggcacc cgcctcgcgg
     1681 gcctgggccg gcggcagcag gtgctcgagc gcgagcgcgt cggcctggtc gagcagcacc
     1741 acgcccttgc cctgctcgcg ggccaggccc agcacctcct gcagcagctc gccggtgttg
     1801 gggccgaact tggaggtgag cgtgccgagc cgcaggtggt agaagaccgc gcccgcctcg
     1861 gtggcgagcg cgcgcgcgag cagggtcttg ccggtgccgg gcgggccgta gatcagcatg
     1921 cccttgggcg gcgcgatgcc ccacttgccg tagagctcgg ggtcggtgag cgcggtcacg
     1981 aagctccgca ccaggccctt ggcctcggcc aggcccgcga tgtcggcgaa cgacgtctcg
     2041 gccttgaccg ccacgtccac gcgcccggtc agctcggcga gcttctcccc gagcttcccg
     2101 aagatctcgc cgaggcgacg ctggatcgcc cactgctggc cctcgacgtc ggccggcgcg
     2161 gccgcggatc cgtcgcgcgg ggtctcggag gccacgcgcc tagatccgct cgccggggcc
     2221 cgcggggccc ccgggcagcc gctcggactt ctcgaagacg cgatcctccg cgaacaccgg
     2281 ggccttcgcc cggatggcga gcgcgatcgc gtcggacggc cgcgagtcga gctccatcgg
     2341 cgcgccgtcc accgtcaggt ggagcgtcgc gtagtaggtg ttgttccgca gatcggtgat
     2401 gaccacccgg gtcaccccga ccttgagccg gccgaagagg gtcaggaaca ggtcgtgggt
     2461 cagcggccgc ggcggggtga cgtgctcgag cggcagcgcg atcgcggtgg cctcggcggg
     2521 gccgatgacc atcagcacgc gacgcttgtc gcgcttgccc tcgatcatga ccccgtgctg
     2581 cccctgcggg tcgctgtaga cgccgaccac cgagatctcc tgcggcccgc ccaccggcgg
     2641 cgccgcgatc tcggtcggct tgcgcgcgcc cgacagcatc acgcccaggc cgagcaccag
     2701 gacgaagcgg ctgagtcggc ggatctgcat catggtcgac cggacctccc cggaaacgtg
     2761 gtcgcacgca gtatcgacct cccccaccgg gcaagtcaac cgcgcgccgc tccggagacc
     2821 gcgggcgagg gcaccgcgta gcccacccgc agccgcgtgc tcaggagcga gcccaccgcg
     2881 agcagcacgg tcagcgcgct ccacatgatc gcgtagccca gcccgtgcgc cacgtacccg
     2941 aagatcaccg ccccggtggt attgccgagc aggatgaccg agctgaagat gcccaccgcg
     3001 ctgccccggc gccgctcgag cgtcacgtcc atgagcagcg ccgacatcgc ggggtacagc
     3061 cagccgtggg ccgcgccggc caggaagccg gccaggaaca ggaacggcag caccggcagc
     3121 cgcagggacg gccagaagag cagcgccacg atcgcgatga tgcaggtcgc ggcggcctgc
     3181 acgaacatgc acgggatgat caccgcgcgc cggccgcgca cgtcgatcag ggtgccgccc
     3241 atcacccgca ccagcatcgc ggccaccgcg tacgcggtgt agaagaggcc gacgctcgcg
     3301 accccgaggg tctccccgaa ggtgggcagg aaggtgaaga tggtcccggt ccccagcccg
     3361 aagaagaacc cgagcaccat gtgcatgtgg cgcagctcga gcaggccttc ccggatgacg
     3421 tgcaggccgg ggcgcgcgcc gagcgcggcg ggccggacgt cgcgcacgtt ccagcacacc
     3481 gcgagcgcgc cgcccgcgat gacggtggcc gccgcgaaga accacgggaa tccgaagcgg
     3541 cggatcatga gctcgccgaa gagcggggcc agcgaggtgg agacgaggcc ggacagcccg
     3601 aagatgccga gggcccagcc cctccgctcg gccggcacca tctccaccac gtggatgtag
     3661 ttggcgacga agaacgcgga gaaggccacc ccctgcagca cgcgcagcgc gcccaggagc
     3721 ggtagcgggc ttgacaccac gaagagcgcg caggagacca ccaggagccc gaccccgagg
     3781 cgcatgaaga accgccgccc gagatagtcg atgagggcgc cgaccaccgg ctggcacacg
     3841 atgccggccg cgctgtagaa cccctgcacg agcccgatgt ccgcctcgtc gcctcccagc
     3901 tgctggatgt agagcggcag cagcaggaac ccgttgaggg cgccgaagaa gaagaagttg
     3961 gtgagcatga tccgcgcgaa cggaccatgt ctcaggaatt cggcggcggg accggtcacc
     4021 cgcgttgcgg ccatatccac ggcgacagta gaatgaccgg cgacatgaac gctcgcgagt
     4081 atagcacgcg gtcgcgcgcg ggccggacgc ccgagaccgt cgcgcaggtg atggccgccg
     4141 ccgcgcgcct cgagcccgac gggcgcggcc gcctcctcgc gcccgcgtcg ctgcagagct
     4201 ggccgggcat cgtccacggc ggcgggctcg tggccctgct ggacgcggcc gcgcgcgcgc
     4261 tcggcgcggg cgacggaccg cgccgcctcg agggccgcct cacctcgtcg gtaccgatcg
     4321 cgaccgcgct cgatctcgag ggccacgcgg agcacgacgc c
//
LOCUS       DATMCV010000169         2239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_207874, whole genome shotgun sequence.
ACCESSION   DATMCV010000169 DATMCV010000000
VERSION     DATMCV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207874"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VNU03_04030"
     CDS             <1..785
                     /locus_tag="VNU03_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWP73651.1"
                     /translation="FLTVLLFRRGWEEFRELRAADLRTFAFLGFVGLFVSTGGTYLGI
                     ALSTAANAAILQAATPVMVALGARFYLGERLRRRQWAGVAASMAGVLLVITRGSWRAI
                     AHLDLLPGDFILLVSQVGWSAYTIYGKQVLAVHSPAVATTASYILGSAMLLPMMLVAA
                     PFFPRPDFTSQTAWLVVLYQAFLGAVAHVWWYEGVKAVGPSRAAIFLNLQPIVGVLLA
                     WVLLREAIEWPEVGGGALVLLGVALTTQAPLAPPASPPSRAS"
     gene            complement(769..1245)
                     /locus_tag="VNU03_04035"
     CDS             complement(769..1245)
                     /locus_tag="VNU03_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1090..1092),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWP73652.1"
                     /translation="MSAMSEGKAPGVGDPAPDFTLKTIGLKDVSLKTYRGKNVVLLFY
                     PLDWTPGUSTCMPAFDRRIRDFEAANTQVMGISTDSPFSHENWAKSVGISHYPLLSDI
                     QRSAVRAYGVYWPDWNANVRATFVIDTEGVIRFTERYGKGELPDPDKILAEVKKLG"
     gene            complement(1242..2198)
                     /locus_tag="VNU03_04040"
     CDS             complement(1242..2198)
                     /locus_tag="VNU03_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73653.1"
                     /translation="MIVTPLAADSLGVRSVATYVECGQTRVLIDPGAVLAPSRFNLPP
                     VEEEWEALRRANDRISTYATRASLIFVSHYHEDHFRHDPGLYAGRHVWAKDPKRHIAG
                     RQAQRAERFWSVIGGSCPTDAAEGRRLETVDAVLQASPPLSHGPDGTGLGYVVALTVE
                     DRREGTRFVHASDVQGPLSAVAAAYLARQRPTLLYLSGPPAYLESQLGAELVDRGIEH
                     LLRIIDQTGCRVILDHHALRDAAYPERFRRLWETGRVVTAAGYLGERDAPLEARRREL
                     WTGRRKPPMPVRPSRARGVVPGEDRASEATNFRPRFGRVREG"
BASE COUNT          281 a          823 c          782 g          353 t
ORIGIN      
        1 cgttcctcac cgtgctgctc ttccgccgcg ggtgggagga gttccgcgag ctacgcgcgg
       61 ccgacctccg cacgttcgcg ttcctcggct tcgtgggcct tttcgtctcc accggcggca
      121 cctacctcgg catcgcgctc agcaccgcgg ccaacgcggc gatcctccag gccgcgactc
      181 cggtgatggt cgctctcggc gcccgcttct acctggggga gcgcctgcgc cggcgccagt
      241 gggcgggagt ggccgcctcg atggcgggag tgctgctggt catcacccgc gggagctggc
      301 gggcgatcgc ccacctcgac ctgctgccgg gcgacttcat cctgctcgtc tcgcaggtgg
      361 gctggagcgc ctacaccatc tacggcaagc aggtgctggc ggtgcactcg cccgcggtgg
      421 ccaccaccgc ctcctacatc ctcggctccg cgatgctgct gccgatgatg ctggtggcgg
      481 ctccgttctt cccccgcccc gacttcacct cgcagaccgc gtggctggtc gtcctctacc
      541 aggccttcct cggcgcggtc gcccacgtgt ggtggtacga gggggtgaag gcggtgggcc
      601 cgagccgcgc cgcgatcttc ctgaacctgc agccgatcgt gggcgtgctg ctggcgtggg
      661 tgctgctgcg cgaggccatc gagtggccgg aggtgggcgg cggcgcgctg gtcctgctgg
      721 gggtggccct caccacccag gccccgctcg cgccgcccgc ttctccgcct agccgagctt
      781 cttgacctcg gcgaggatct tgtcgggatc gggcagctcg cccttgccgt agcgctcggt
      841 gaagcggatc acgccctccg tgtcgatgac gaaggtggcg cgcacgttcg cgttccagtc
      901 cggccagtag acgccgtagg cacggaccgc ggagcgctgt atgtcggaca gcagcgggta
      961 gtgggagatg ccgaccgact tcgcccagtt ctcgtggctg aacgggctgt ccgtgctgat
     1021 acccatgacc tgggtattcg ccgcctcgaa gtcgcggatg cgacggtcga aggcgggcat
     1081 gcaggtggat cagcccgggg tccagtcgag cgggtagaac aggagcacca cgttcttgcc
     1141 ccggtaggtc ttgaggctca cgtccttgag cccgatcgtc ttcagcgtga agtcgggagc
     1201 gggatcgccc accccgggtg ccttgccctc gctcattgcg ctcatccctc tctcacccgc
     1261 ccgaagcgcg ggcggaagtt ggtcgcctct gacgcgcggt cctcgccggg gacgaccccg
     1321 cgcgcccgcg acgggcgcac cggcatcggc ggcttgcgcc gccccgtcca cagctcgcgg
     1381 cggcgcgcct cgagcggcgc gtcgcgctcc cccaggtaac ccgccgcggt gaccacgcgc
     1441 ccggtctccc acagccggcg gaagcgctcc gggtacgccg cgtcccgcag cgcgtggtga
     1501 tcgaggatca cccggcagcc ggtctgatcg atgatccgca gcaggtgctc gatcccccgg
     1561 tccaccagct cggcgcccag ctgcgactcg agataggcgg gcggccccga caggtagagc
     1621 agcgtcggac gctgccgcgc cagataggcg gcggccaccg cggacagcgg gccctgcacg
     1681 tcggaggcgt gcacgaagcg cgtgccttcc cggcgatctt ccaccgtcag ggcgaccacg
     1741 tagccgagcc cggtgccgtc cggcccatgg ctcagcggcg gcgaggcctg cagcaccgcg
     1801 tccaccgtct cgagccgccg gccctccgcg gcgtcggtgg ggcacgagcc cccgatcacg
     1861 ctccagaacc gctccgcgcg ctgggcctgc cggccggcga tgtgccgctt cggatccttg
     1921 gcccagacgt gccggcccgc gtagagcccg gggtcgtggc ggaagtggtc ctcgtggtag
     1981 tggctgacga agatcagcga cgcccgcgtc gcgtaggtcg agatgcgatc gttcgcgcgg
     2041 cgaagggcct cccactcctc ctcgacgggt gggaggttga agcggctggg ggcgagaacc
     2101 gcgcccggat cgatgaggac gcgcgtctgc ccgcactcca cgtacgtggc caccgagcgc
     2161 acccccagcg agtcggccgc gagcggggtg acgatcatcg agtcccctca ccaccggacc
     2221 cctcgtcacc gaacccctc
//
LOCUS       DATMCV010000170         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_875054, whole genome shotgun sequence.
ACCESSION   DATMCV010000170 DATMCV010000000
VERSION     DATMCV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875054"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VNU03_04045"
     CDS             <1..244
                     /locus_tag="VNU03_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWP73654.1"
                     /translation="FIGVMEILGGFGLVLPSLLRIRPGLTPLAAACLVIIMIGATVIT
                     LTAMGVGAAILPLIVGILCVFVAYGRWRLAPLSPRR"
     gene            303..1520
                     /locus_tag="VNU03_04050"
     CDS             303..1520
                     /locus_tag="VNU03_04050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWP73655.1"
                     /translation="MTSRSGGFSKTRLGRMRDVMTGHVERGAMPGLVTLVSRRDEVHV
                     EPLGRMAADDSRPMERDTIFRIASMTKPVAAAAAMILVEECRLRLDEPVDRLLPELAA
                     RRVLRRLEGPVDDTVPASRSITLRDLLTFRLGFGIIMAPPDTYPIQKAMREKGMRMGP
                     PRPQAEPAPDTWMQSLGTLPLLHQPGERWMYDTGSDVLGVLIARAAGQPFDTFLRERL
                     FEPLGMKDTGFSVPAGKIGRLATSYWSGPKSGAMEVYDPAEGGEWSRAPAFPSAAGGL
                     VSTVDDYLAFGRMLLNRGRHGAERILSRPSVEAMTTDQLTPEQKALSGFGPGFWDSRG
                     WGFGLSVITRRDGVAAVPGRFGWDGGLGTSWSSDPTEELVGILMTQRMWTSPQGPDVY
                     HDFWTQAYQAIDD"
     gene            1561..1989
                     /locus_tag="VNU03_04055"
     CDS             1561..1989
                     /locus_tag="VNU03_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWP73656.1"
                     /translation="MRFMMLVKASQDSEAGVMPNKGLVAEMGELNEEMAKAGVLLAGE
                     GLHPSSKGARVTYTGRKRLVTDGPFAETKELLAGFWMIQVASRAEALEWASRIPFTDG
                     EVVEVRQVFEASDFPADVLPPEAAAREEALRAELAKKAKR"
     gene            complement(2003..>2475)
                     /locus_tag="VNU03_04060"
     CDS             complement(2003..>2475)
                     /locus_tag="VNU03_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWP73657.1"
                     /translation="IYRHYDTVAHELSLEHLVEEPPVNNTVLVLVGDLHMGVVKALRY
                     AQSLSPSPKAVYVELDPSATARLEERWSKGGCGVPLVVLASPYRSMLRPLLDYIGRIR
                     ERDANSVVTIVIPEFVPRRWWQHLLHNQTALLVKGALLFRRGVVVVDVPFHLHS"
BASE COUNT          381 a          816 c          906 g          372 t
ORIGIN      
        1 tttcatcggg gtgatggaga tcctcggggg attcggcctg gtcctgccca gcctcctgcg
       61 tatccggccc gggctgaccc cgctcgccgc cgcgtgcctc gtgatcatca tgatcggggc
      121 gaccgtgatc acgctcaccg cgatgggcgt cggcgccgcg atactcccgc tgatcgtcgg
      181 gatcctctgc gtgttcgtcg cctacggtcg ctggcgacta gccccgcttt cgccccgccg
      241 ctgatgctca ccgcgctgtc gatcctgagc ggacgagagc gaccagtggg caggaggaca
      301 cgatgacatc gaggagcgga ggattctcca agacgcggct cgggcgcatg cgcgacgtga
      361 tgaccggcca cgtggagcgt ggcgccatgc ccgggctggt cacgctggtg agccgacgag
      421 acgaggtgca cgtcgagccg ctcggccgca tggccgccga tgatagccgg ccgatggagc
      481 gcgatacgat cttccgcatc gcctccatga ccaagccggt cgcggccgcc gcggcgatga
      541 tcctggtgga ggagtgccgg ctgcggctcg acgagccggt ggatcggctg ctgcccgagc
      601 tcgccgcccg ccgggtgctg cggcggctcg aggggccggt cgacgacacg gtgcccgcga
      661 gccggtcgat caccctgcgc gatctgctga ccttccgcct gggcttcggc atcatcatgg
      721 cgccgcccga cacgtacccg atccagaaag cgatgcgcga gaagggaatg cggatggggc
      781 cgcctcggcc gcaggcggag cccgcgcccg acacgtggat gcagagcctc ggcacgctgc
      841 ccctgctgca tcagccgggc gagcggtgga tgtacgacac cggctcggac gtgctgggcg
      901 tgctgatcgc gcgggcggcg ggacagccgt tcgacacgtt cctgcgcgag cgcctcttcg
      961 agccgctcgg gatgaaggac accggcttca gcgtgcccgc cggcaagatc gggcgtctcg
     1021 ccaccagcta ctggagcggc ccgaagagcg gggcgatgga ggtctacgac ccggccgagg
     1081 gcggtgagtg gagccgcgcg cccgcgtttc cctccgccgc cggcgggctg gtgtcgaccg
     1141 tcgacgacta tctcgccttc ggccggatgc tgctgaaccg cggccgccac ggcgcggagc
     1201 gcatcctgtc acgcccctcg gtcgaggcga tgacgaccga tcagctgaca cccgagcaga
     1261 aggcgctctc cggcttcggg cccggcttct gggacagccg cgggtggggc ttcgggctgt
     1321 cggtgatcac ccggcgcgac ggcgtggccg cggtgcccgg acgattcggg tgggacggag
     1381 gtctcggtac ctcgtggtcg tcggatccga cggaagagct ggtcggcatc ctgatgaccc
     1441 agcgcatgtg gacatcaccg cagggcccgg acgtgtatca cgacttctgg acgcaggcct
     1501 accaggccat cgacgactga gaccacgagc tattcacgag ctaattgggg agatattgcg
     1561 atgcgattca tgatgctggt gaaggcgagt caggattcgg aggcgggcgt gatgcccaac
     1621 aaggggctcg tcgccgagat gggggagctc aacgaggaga tggcgaaggc cggcgtgctg
     1681 ctggccggcg aggggctcca tccgagctcc aagggcgcgc gcgtcacgta cacgggccgc
     1741 aagcgcctcg tgaccgacgg gcccttcgcg gagacgaagg agctgctggc cggcttctgg
     1801 atgatccagg tggcgtcgcg ggcggaggcg ctcgagtggg cctcccgcat tccgttcacc
     1861 gacggcgagg tcgtcgaggt tcgccaggtc ttcgaggcct ccgactttcc cgcggacgtc
     1921 ttgccgcccg aggccgccgc gcgcgaggag gcgctccgcg cggagctcgc gaagaaggcg
     1981 aagcggtagt cagctgtgcc catcagctat gaagatggaa gggcacgtcg accaccacga
     2041 cgccccggcg aaacaggagg gcgcccttca ccaggagcgc ggtctggttg tgcagcaggt
     2101 gctgccacca ccgccggggc acgaactcgg ggatgacgat ggtcaccacc gagttcgcgt
     2161 cgcgctcccg gatccgaccg atgtagtcga ggagggggcg gagcatcgag cgatacggtg
     2221 aggcgagcac caccagcggc accccgcagc cacccttcga ccagcgctcc tccaggcggg
     2281 cggtcgcgct cgggtcgagc tccacgtaga ccgccttcgg gctcggggag agcgactggg
     2341 cgtagcgcag ggccttgacc acgcccatgt ggagatcgcc gaccaggacc agcaccgtgt
     2401 tgttcaccgg cggctcctcg acgagatgct cgagggagag ctcgtgggcc accgtgtcgt
     2461 agtgccggta gatcg
//
LOCUS       DATMCV010000171         3847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_143778, whole genome shotgun sequence.
ACCESSION   DATMCV010000171 DATMCV010000000
VERSION     DATMCV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143778"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /gene="gcvPA"
                     /locus_tag="VNU03_04065"
     CDS             complement(<1..1038)
                     /gene="gcvPA"
                     /locus_tag="VNU03_04065"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWP73658.1"
                     /translation="MRYISNTPAQQKEMLALIGASSIEDLLVRIPAKARLSRPLNLPG
                     AMAETDLVRHLQRLAARNAGADDYACFLGAGSYDHAIPSPINHLISRGEFFTAYTPYQ
                     PEASQGTLRSIFEYQTMMAELTGMDVANASIYDGASSLAEATLMAHSVTGRDTLVISR
                     GVNPLYRQVVETYAEGPGLKIKSAPLGDGVTDLDAVRKAVSKHTAAVVIQYPNFFGCL
                     EDVRAAADCAHEAGALLVVVADPINLGLLTPPGACGADIVVGEGQGLGVPMSFGGPNL
                     GVFAVKQELVRRMPGRLVGATVDVDGQRGFVLTLQTREQHIRREKATSNICTAQVLLA
                     VIASMYAVYHGP"
     gene            complement(1055..1447)
                     /gene="gcvH"
                     /locus_tag="VNU03_04070"
     CDS             complement(1055..1447)
                     /gene="gcvH"
                     /locus_tag="VNU03_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWP73659.1"
                     /translation="MANVPAELRYTREHEWAKVEGDRARVGITAFAQEQLGDVVFVEL
                     PKVGAKVTAMKTFGVVESVKAVSDLFAPVTGEVVEVNAELPKKPEVVNTDPYGQGWML
                     VIRMSNPKEADGLMSAADYEKLTAGAGH"
     gene            complement(1460..2581)
                     /gene="gcvT"
                     /locus_tag="VNU03_04075"
     CDS             complement(1460..2581)
                     /gene="gcvT"
                     /locus_tag="VNU03_04075"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWP73660.1"
                     /translation="MTDATASTPLKHTPLRDVHVKAGAKMVPFGGWDMPVQYTGIIDE
                     HRTVRGAVGLFDISHMGEFEVRGADALAAVQRLCTNDASTLAIGQVQYAALCYPEGGI
                     VDDLTVYRLAPDHFMLVVNASNIEKDWAWVTSHSEGRAQWANVSDETALLAVQGPKAE
                     ALVARLADRDVIGIDYYHFARGKVAGVDGLISRTGYTGEDGFELYVPAGQAERLWAAL
                     LEAGRGDGIKPIGLGARDTLRLEMKYALYGNDIDQTTNPLEAGLGWVVKPAKGEFLGR
                     GAVEALRAGGVGRKLVGFEMAERAVPRHGYRLLAGGAPVGVVTSGSFGPSVERSIGMG
                     YVASPQTAVGTALTVEIRGALHPARVVKTPFHPSHTKKA"
     gene            complement(2600..3046)
                     /locus_tag="VNU03_04080"
     CDS             complement(2600..3046)
                     /locus_tag="VNU03_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73661.1"
                     /translation="MAGRAATRRLAVALVAAVAACGCGATKLPPTAALRDQTATQQRL
                     DAIDCRAEMGSRLDYNGDDSEALNVLRHVFTLGAGGAAIGGLATGFSPATGVSSASEG
                     LIAGSGAGVIAGGVLGIGGRSRFERAWIACMESRGYAIVAPSGPIQ"
     gene            complement(3046..3819)
                     /locus_tag="VNU03_04085"
     CDS             complement(3046..3819)
                     /locus_tag="VNU03_04085"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HWP73662.1"
                     /translation="MPKTIDLNCDMGESYGRWTLGADEAIMPLISSANVACGFHGGDP
                     HVMRKTVELALAHGVALGAHPSLPDLMGFGRRKMDVSPRELQDYFVYQTGALRELARA
                     AGAELQHMKPHGILYGMMETDEALATAAGQSAMDSGLILMTLASGRYDATCRKMGARV
                     ASEGFADRAYNVDGTLVSRKIAGSLITDPARAAAQAVLMATEGKACTIDGVDIDISVQ
                     TICCHGDTPGAERIVRAVREALEKAGCQVKPLRDWLPAH"
BASE COUNT          529 a         1389 c         1346 g          583 t
ORIGIN      
        1 tgggccatgg tagaccgcgt acatcgacgc gatcaccgcg agcagaacct gcgcggtgca
       61 gatgttcgac gtcgccttct cgcgccggat gtgctgctcg cgcgtctgaa gcgtgagcac
      121 gaagccgcgc tgcccgtcga cgtccacggt ggcgcccacc aggcggccgg gcatccggcg
      181 caccagctcc tgcttgaccg cgaacacccc caggttggga ccgccgaagc tcatcggcac
      241 gccgaggccc tgcccctcgc ccaccacgat gtcggcgccg caggccccgg gaggagtgag
      301 caggccgaga ttgatcggat cggccaccac caccagcagc gcgcccgcct cgtgggcgca
      361 gtccgccgcg gcgcggacgt cctcgaggca gccgaagaag ttggggtact ggatcaccac
      421 cgcggcggtg tgcttggaca ccgccttccg caccgcgtcg agatcggtca ccccgtcgcc
      481 gaggggggcc gacttgatct tgagcccggg cccctcggcg taggtctcca ccacctgccg
      541 gtagagcggg ttgaccccgc gcgagatcac gagggtgtca cgcccggtca ccgagtgggc
      601 catcagcgtg gcctcggcca gcgaggaggc cccgtcgtag atggaggcgt tggccacgtc
      661 catcccggtc agctccgcca tcatggtctg gtactcgaag atcgagcgca gggtgccctg
      721 gctcgcctcg ggctggtacg gggtgtaggc ggtgaagaac tcgccgcgcg agatcaggtg
      781 gttgatgggg ctcgggatcg cgtgatcgta ggagccggcg ccgaggaagc acgcgtagtc
      841 gtcggcgccc gcgttccgcg cggcgagccg ctgcaggtgc cgcaccaggt cggtctcggc
      901 catcgcaccc ggcaggttga gcggccgcga gagccgcgcc ttggccggga tgcggaccag
      961 caggtcctcg atcgaggagg ccccgatgag ggcgagcatc tccttctgct gggcgggcgt
     1021 gttcgagatg tagcgcatgc cgtccccttt tctcctagtg gccggcgccc gcggtgagct
     1081 tctcgtagtc ggccgcgctc atgagaccgt ccgcttcctt ggggttgctc atccggatca
     1141 ccagcatcca gccctgcccg tagggatccg tgttcaccac ctccggcttc ttgggcagct
     1201 ccgcgttgac ctcgacgact tccccggtga ccggggcgaa caggtccgag accgccttca
     1261 ccgactccac caccccgaag gtcttcatgg cggtgacctt cgcgcccacc ttcggcagct
     1321 ccacgaagac cacgtcgccg agctgctcct gggcgaaggc ggtgatgccg acgcgggccc
     1381 ggtcgccctc caccttggcc cattcgtgct cgcgcgtgta cctgagctcc gccggaacgt
     1441 tcgccatcga ctcttctccc taggccttct tggtatgcga gggatggaac ggggtcttca
     1501 ccacgcgggc cgggtgcagg gcaccccgga tctcgactgt cagagcggtg cctaccgcgg
     1561 tttgcggcga cgccacgtag cccatgccga tcgagcgctc gaccgacggg ccgaacgacc
     1621 cggaggtgac caccccgacc ggcgcgccgc ccgcgagcag gcggtagccg tgccgcggca
     1681 ccgcgcgctc ggccatctcg aagcccacca gcttgcgccc gaccccgccc gcgcgcagcg
     1741 cctcgaccgc gccccgcccg aggaactcgc ccttggccgg cttcaccacc cagccgaggc
     1801 cggcctcgag cgggttggtg gtctggtcga tgtcgttgcc gtagagggcg tacttcatct
     1861 cgagcctcag cgtgtcgcgc gcgccgagcc cgatcggctt gatgccgtcg ccgcgccccg
     1921 cctcgaggag cgcggcccag agccgctcgg cctggccggc cggcacgtac agctcgaagc
     1981 cgtcttcccc ggtgtagccg gtgcgggaga tgaggccgtc gaccccggcc accttgccgc
     2041 gcgcgaagtg atagtagtcg atgccgatca cgtcgcggtc ggcgagccgg gccaccagcg
     2101 cttccgcctt cgggccctgc accgcgagga gcgcggtctc gtcgctcacg ttcgcccact
     2161 gggcgcgccc ctcgctgtgc gacgtcaccc acgcccagtc cttctcgatg ttggaggcgt
     2221 tcaccaccag catgaagtga tcgggagcga ggcggtagac ggtcaggtcg tccacgatgc
     2281 cgccctccgg gtagcagagg gccgcgtact ggacctggcc gatcgcgagg gtggaggcgt
     2341 cgttggtgca gagccgctgg accgcggcga gggcgtcggc gccgcgcacc tcgaactcac
     2401 ccatgtggct gatgtcgaag agcccgaccg cgccgcggac cgtccggtgc tcgtcgatga
     2461 tcccggtgta ctgcaccggc atgtcccagc cgccgaaggg gaccatcttg gcgcccgcct
     2521 tgacgtgcac gtcgcgaagc ggggtgtgct tgagcggagt cgaggcggtc gcgtcagtca
     2581 tcagatcggc gccgtcatct tactggatgg ggccggacgg ggcaacgatc gcgtacccgc
     2641 ggctctccat gcaggcgatc cacgcgcgct cgaagcgcga gcggccgccg atgccgagga
     2701 caccgcccgc gatgaccccg gcccccgagc ccgcaatgag cccttcgctg gccgacgaca
     2761 ccccggtggc gggggagaac ccggtggcga ggccgccgat cgcggcgcct cccgcgccga
     2821 gggtgaacac gtggcgcagc acgttgaggg cctcggagtc gtcgccgttg tagtccaggc
     2881 gagagcccat ctcggcccgg cagtcgatcg cgtcgagtcg ctgctgggtc gcagtctggt
     2941 cgcgcagcgc ggcggtcggc ggaagcttcg tggccccgca gccgcacgcc gccaccgccg
     3001 ccaccagggc gaccgccagc cgccgggtcg ccgcgcggcc ggccatcagt gggcgggcag
     3061 ccagtcgcgc agcggcttga cctggcagcc cgccttctcc agcgcctcgc gcaccgcgcg
     3121 cacgatgcgc tcggcgcccg gggtgtcgcc gtggcagcag atggtctgga ccgagatgtc
     3181 gatgtcgacc ccgtcgatgg tgcacgcctt gccctcggtg gccatcagga ccgcctgggc
     3241 cgcggcccgg gccgggtcgg tgatgagcga gcccgcgatc ttgcgcgaga cgagggtgcc
     3301 gtccacgttg taggcgcggt ccgcgaagcc ctccgaggcc acccgcgcgc ccatcttgcg
     3361 gcaggtcgcg tcgtagcgtc cggaggcgag cgtcatcagg atgaggcccg agtccatcgc
     3421 ggactggccc gccgcggtgg cgagggcctc gtcggtctcc atcatgccgt agaggatgcc
     3481 gtggggcttc atgtgctgca gctcggcccc cgcggcgcgg gccagctcgc gcagcgcgcc
     3541 ggtctggtag acgaagtagt cctgcagctc gcgcgggctc acgtccatct tgcgacggcc
     3601 gaagcccatg aggtccggga gcgaggggtg cgccccgagg gcgaccccgt gggcgagcgc
     3661 cagctctacc gtcttgcgca tcacgtgcgg gtcgccgccg tggaagccgc acgcgacgtt
     3721 ggccgagctg atgagcggca tgatggcctc gtcggccccg agcgtccagc gtccgtacga
     3781 ctcgcccatg tcgcagttga gatcgatggt cttcggcatg tcattcctca ccccactcca
     3841 cccctca
//
LOCUS       DATMCV010000172         4472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_742328, whole genome shotgun sequence.
ACCESSION   DATMCV010000172 DATMCV010000000
VERSION     DATMCV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742328"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..937
                     /locus_tag="VNU03_04090"
     CDS             254..937
                     /locus_tag="VNU03_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWP73663.1"
                     /translation="MRESPETAALVGAVAARLREARRAQGLTLRALSEKTGLSEPFLS
                     KLERGRVSTSIANLILISRTVGIELGQLFQDAAGTTATQHYALVRAGERRAPQAVPAT
                     GYTYQPMAVGWPGQRMDAFVLTFPVRNRADVLTAHEGEELSFVLQGEILFQLGDEKIP
                     LKAGDCLYFNAEIPHMGKNVGRIDAKVLMVAAPGRGPGREFGWWKGPAPAPAVRTRRG
                     KPTRTRSGD"
     gene            939..2897
                     /locus_tag="VNU03_04095"
     CDS             939..2897
                     /locus_tag="VNU03_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP73664.1"
                     /translation="MERTIYTSCTLDCPDGCGIIAHVKDGRVVKLEGHPDHEFTQGYL
                     CGKTYRFPERVYSPERQLHPLKRENGRPDGGWVRIGWDEALDLIAEKIRGHVAEHGPL
                     SIMHYQRTGSWGATKLLSRRFWNLLGGATTTSGSLCSGAARAGQALDFGVRAGHDPSD
                     MLNSRLVLLWGRNPMATNLHTVPILKEVRRRGGRVILIDPVRSESANFCDAHVQPRVA
                     TDAELAMAMAKVILEEGLEDRGFLDRHAHGFAQYRALLDARPLAELAAACELSEAAIR
                     ELAREYATTRPASILLGWGLNKYQHSAEIFRCVDALGALGGHIGVPGGGVSHGYNTQR
                     HFDKTVEAADRARHHRAIPEPLLGRGLIEAQKPPIRMMFVNGGNPVNQSPNSNLVAKG
                     LAGLDFLVVVDQVLTDTTDYAHVFLPTTTFLEEEDMLVSWGHNIIGGVNQVIEPVGES
                     RSDLLIFQQLADRLGIGPEMAGAPRDWLERIFTPLTNRGVSVEQVMEGPVRCPVAPMV
                     PFADHVYPTKSGRFEFITQLRHQPRVVPDFPLTLVTNYSKKWLLSQILEKEHPSIASV
                     RIGTETAAAYGIENGERAIIRSPVGHLEVEVLVDARIGRGMVIMPVGTWIKRGGGANV
                     LTEDIMSNFGQMAAFGETRVRLERLVTA"
     gene            2953..3792
                     /locus_tag="VNU03_04100"
     CDS             2953..3792
                     /locus_tag="VNU03_04100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWP73665.1"
                     /translation="MANPLSRREVLKYGAFTTVGGVLTPGISLAQAITTESRGLTARD
                     IQLQSGGTAIPAYDARPEAAGRYPILVVIAGFTGNSEQHKDVVRRFAQAGYYAIAPEL
                     YSREGGMQGKNFQEMGQIAAKVTRAQYLGDIRAAADYAKQQTWARADRLGVTGFCGGG
                     ALTLHTAAEYPGVTAAVPWYGQVKRTYSDAPGVDAFSLTARITAPVLGLYGEADPGIP
                     ADDVKRFEAELKKTNPNVEFILYPGAQHAFFSDDRPQAYQKAAAEDGWKRCLAFFDKH
                     LKS"
     gene            3893..>4472
                     /locus_tag="VNU03_04105"
     CDS             3893..>4472
                     /locus_tag="VNU03_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73666.1"
                     /translation="MPTSHRARIHRRDLLALGAGALAVSALPGGLAPRAAWAQAPRPG
                     GTLSLRLWDPPHWDPHLTVSYKTHIAYSFTHSRLLKHKAGPAIPAGTFPIEGDLAESW
                     SQPSETTYVFKLRKGVRWQNRPPVNGRELTAEDVVYSVERFRTLKGNANAYMLSSLEK
                     VEAIDKHTIKFMLKEPYVWFLDMIANSHAVPII"
BASE COUNT          725 a         1613 c         1496 g          638 t
ORIGIN      
        1 ccgatgccgc tgtaggtgtt gatgtcgccg atcttgatcg gcgcctgggc cgcggcgggg
       61 cctgctccga gcgtgagcag cagggccgcc gccgtgaggg acaggagacg tggtgcgcgc
      121 atgcgaagcc tcctccgtgg atcggtcgtg ttgcgcctga gtaaagcaaa agcgcggaag
      181 ctgtcaagcc gattctcccc gcttgacagg gagcataccc gagtggtttc ctcaggggca
      241 agcggagatt gccttgagag aaagtccgga gacggcggcg ctggtggggg cggtggcggc
      301 gcgcctgcgc gaggcgcggc gggcccaggg cctgaccctg cgcgcgctca gcgagaagac
      361 cgggctgtcc gagcccttcc tctccaagct cgagcgcggg cgcgtttcca cctcgatcgc
      421 gaacctgatc ctgatcagcc gcaccgtcgg catcgagctg gggcagctct tccaggacgc
      481 ggcgggcacc accgcgaccc agcactacgc gctggtgcgg gccggcgagc gccgcgcccc
      541 ccaggcggtt cccgccaccg gctacacgta tcagccaatg gcggtcggct ggcccggcca
      601 gcgcatggac gccttcgtgc tcaccttccc ggtgcggaac cgggccgacg tgctcaccgc
      661 ccacgagggc gaggagctgt cgttcgtgct gcagggcgag atcctgttcc agctcggcga
      721 cgagaagatc ccactcaagg cgggcgactg cctctacttc aacgcggaga tcccccacat
      781 gggcaagaac gtggggcgga tcgacgccaa ggtgctcatg gtcgcggcac cgggacgcgg
      841 tcccgggcgc gagttcggct ggtggaaggg gcccgcgccc gcccccgccg tccgcacgcg
      901 gcggggcaag ccaacgagga caaggagcgg ggactgacat ggagcggacg atctacacct
      961 cgtgcacgct cgactgcccg gacggctgcg gcatcatcgc ccacgtgaag gacgggcggg
     1021 tggtgaagct cgaaggccac ccggatcacg agttcaccca gggctatctc tgcgggaaga
     1081 cctaccgctt cccggagcgg gtctacagcc ccgagcggca gctccatccg ctcaagcgcg
     1141 agaacggccg cccggacggg ggctgggtgc gcatcggctg ggacgaggcg ctcgacctga
     1201 tcgcggagaa gatccgcggc cacgtggccg agcacggccc gctctcgatc atgcactacc
     1261 agcgcaccgg ctcgtggggc gccaccaagc tcctgagccg ccgcttctgg aatctactcg
     1321 gcggcgccac caccaccagc ggctcgctgt gcagcggcgc ggcgcgggcg ggccaggcgc
     1381 tcgacttcgg cgtccgcgcc ggccacgacc cgtccgacat gctcaacagc cgcctcgtgc
     1441 tgctgtgggg ccgcaacccg atggccacca acctccacac ggtgccgatc ctgaaggagg
     1501 tgcgccgccg cggcggccgc gtgatcctga tcgatccggt gcggagcgag tcggcgaact
     1561 tctgcgacgc gcacgtgcag ccgcgggtcg ccaccgatgc ggagctggcc atggcgatgg
     1621 ccaaggtcat cctggaggag ggcctggagg accgcgggtt cctcgatcgc cacgcgcacg
     1681 gcttcgctca gtaccgcgcc ctgctcgacg cccgcccgct cgccgagctg gccgccgcgt
     1741 gcgagctgtc cgaggccgcg atccgcgagc tggcccgcga gtacgcgacc acccggcccg
     1801 cctccatcct gctcggctgg ggcctcaata agtaccagca cagcgccgag atcttccgct
     1861 gcgtggacgc cctcggggcc ctcgggggtc acatcggggt gccgggcggc ggcgtctccc
     1921 acggctacaa cacccagcgg cacttcgaca agacggtcga ggccgccgac cgcgcccggc
     1981 accaccgggc gatcccggag cccctgctcg gacgcgggct catcgaggcg cagaagccgc
     2041 ccatccgcat gatgttcgtc aacgggggca acccggtgaa ccagtccccg aactcgaacc
     2101 tggtggccaa gggcctcgcg ggtctcgact tcctggtcgt ggtggaccag gtgctcaccg
     2161 acaccaccga ctacgcccac gtcttcctcc ccaccaccac gttcctcgag gaagaggaca
     2221 tgctggtctc ctggggccac aacatcatcg gcggggtgaa ccaggtgatc gagccggtgg
     2281 gcgagtcccg ctccgatctc ctgatcttcc agcagctcgc ggaccgcctc ggcatcgggc
     2341 cggagatggc gggcgccccg cgcgactggc tcgagcggat cttcaccccg ctcaccaatc
     2401 ggggcgtgag cgtcgagcag gtgatggagg gcccggtgcg ctgcccggtc gcgcccatgg
     2461 tgccgttcgc ggatcacgtc tatcccacca agtcgggccg cttcgagttc atcacccagc
     2521 tgcgccacca gccgcgcgtg gtcccggact tcccgctcac cctcgtgacc aactacagca
     2581 agaagtggct cctctcccag atcctcgaga aggagcaccc gtccatcgcc tcggtgcgta
     2641 tcggcaccga gaccgcggcc gcctatggca tcgagaacgg cgagcgcgcc atcatccgct
     2701 ccccggtcgg gcacctcgag gtggaggtgc tggtggacgc gcgcatcggg cgcggcatgg
     2761 tcatcatgcc ggtgggcacc tggatcaagc gcggcggcgg tgccaacgtg ctcaccgagg
     2821 acatcatgtc caacttcggg cagatggccg cgttcggcga gacgcgcgtg aggctcgagc
     2881 ggctcgtcac cgcctgaccg ggaaccgggg gtcgggccgg cgagttaggt ttggccagga
     2941 ggagaaccca ccatggccaa tccgctcagc cgccgggaag tgctcaagta tggcgccttc
     3001 accaccgtcg ggggcgtgct caccccgggc atctcactgg cccaggccat caccaccgag
     3061 agccggggcc tgaccgcgcg ggatatccag ctccagtcgg gcgggaccgc gatcccggcc
     3121 tacgacgccc ggcccgaggc ggccggccgt tatccgatcc tggtcgtcat cgcgggcttc
     3181 accggcaact cggagcagca caaggacgtg gtgcgccgct tcgcccaggc cggctactac
     3241 gcgatcgccc ccgagctcta ctcgcgcgag ggcggcatgc agggaaagaa tttccaggag
     3301 atgggccaga tcgcggccaa ggtgacgcgg gcccagtacc tgggtgacat ccgcgcggcc
     3361 gccgactacg ccaagcagca gacgtgggcg cgggccgatc ggctcggagt caccggcttc
     3421 tgcgggggcg gcgcgctcac gctgcatacc gcggccgagt atccgggcgt cacggcggcg
     3481 gtcccgtggt acggccaggt caagcgcacc tactcggacg cgcccggagt ggacgcgttc
     3541 agcctgaccg cccgcatcac cgcaccggtg ctcggcctgt acggggaggc ggatcccggc
     3601 atcccggccg acgacgtgaa gcgcttcgag gcagagctga agaagacgaa cccgaacgtc
     3661 gagttcatcc tctatccggg cgcgcagcac gccttcttct ccgacgaccg gccgcaggcc
     3721 taccagaagg cggcggcgga agacggctgg aagcgctgcc tggccttttt cgacaagcat
     3781 ctgaagagct gatccgcccg cttgacagcg agggcgtttc acctcagatt ggcgaaaccc
     3841 gtggtctttc tatccgtgtc tctaacgtca ccgccgaccc ggaggacacc ggatgccaac
     3901 ctcccaccgt gcgcgaatcc accgccgtga cctcctagcc ctcggagccg gcgccctcgc
     3961 cgtttctgcc ctgcccggcg gactcgcgcc ccgggccgcg tgggcccagg ctccacgccc
     4021 gggcggcacc ctgagcctcc gcctctggga cccgccgcac tgggatcccc acctgaccgt
     4081 ttcctacaag acccacatcg cctactcgtt cacccacagc cgcctcctca agcacaaggc
     4141 cggccccgcc attccggcgg gcaccttccc catcgagggc gatctggccg agtcgtggag
     4201 ccagcccagc gagaccacgt acgtcttcaa gctccgcaag ggagtccgct ggcagaacag
     4261 gccgcccgtg aatggacggg agctgacggc cgaggacgtc gtctactccg tggagcgctt
     4321 ccggacgctc aagggcaacg ccaacgccta catgctgtcg tcgctcgaaa aggtcgaggc
     4381 gatcgacaag cacacgatca agttcatgct caaggagccg tacgtctggt tcctcgacat
     4441 gatcgccaac tcccacgccg tccccatcat cg
//
LOCUS       DATMCV010000173         5640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_672599, whole genome shotgun sequence.
ACCESSION   DATMCV010000173 DATMCV010000000
VERSION     DATMCV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672599"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VNU03_04110"
     CDS             <1..228
                     /locus_tag="VNU03_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP73667.1"
                     /translation="AAALAPRIQVNAVAPGVVLLPAGFPRERGRRLAARIPMGRPGRP
                     ADVAEAVRFFATCSDYVTGQVLFVDGGMSVR"
     gene            complement(243..926)
                     /locus_tag="VNU03_04115"
     CDS             complement(243..926)
                     /locus_tag="VNU03_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP73668.1"
                     /translation="MPAGLYLKQVEVGPMQNFVYLIGDRSARECVVVDPAWEIDGIAD
                     LVAADDMRLTGVLVTHTHQDHVGGHLFGHDIPGIPELLGKHPAKVYVHRAEREFLKGF
                     GSDLVKVEAGDTLQVGAIPLTFVHTPGHTPGSQCFLVDGCLISGDTLFIRSCGRTDLP
                     GSDPKEMYTSLTQRLGALPDDTVVLPGHNYGGSQTTIGAEKRENPMMRFASMSEFLGA
                     MGGGRAGRT"
     gene            complement(926..1324)
                     /locus_tag="VNU03_04120"
     CDS             complement(926..1324)
                     /locus_tag="VNU03_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73669.1"
                     /translation="MKALVVAIGFVVAVTGSTAGVVSAGEVLKPTDFQPNPFAMTPPP
                     TPLAPRPASQVPVGPPPGHHRPPPAVSYPCCAPAPATGYWAYQWVPTVATTYVWVPGY
                     VTADGIMVAGGYQPQLVSSGYYQPVWVPGY"
     gene            complement(1391..3163)
                     /locus_tag="VNU03_04125"
     CDS             complement(1391..3163)
                     /locus_tag="VNU03_04125"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWP73670.1"
                     /translation="MSRGRTILVLLILAAGLGGFFYYDTYSLAPRREKAESAKGRIWT
                     VEPKDVERVTVARKGETIRLQRTADGSWEMLEPIKTRGDRGAVDDMVTGLATARMDRE
                     IDANPSKPADFGLDPPEAEVKLEVKGRTEPLVLRVGSKNPTGVWVYAREGGKPAVITL
                     SESIARDATRPLADFRDRTVIAFDRRNVSGIDLDVNGDQIGVVADEPGKWRIVKPRAL
                     RADTDLISEFLDKLEGAKAVEFVDDAPKSLQPYGLDKPSKVTVWVGKDKDRAARTLLV
                     GRPVPEKKGVYVKREGEPGVILTAEAVWTVFPKTVGAIRDKTVVSYASDKLARIEITR
                     GRETLALAKEGTGWKITAPEALRADSGAVTQILWKIRDLQALGFLSEGAAEVPRFLSK
                     PEVIVRLWETGAKEPKTLLLQSSNERRGGQPAALAAVQGEGPVVLVEGKALTELTPSV
                     AQLRDRTVFPTFDLGDVKRARVSGDKPLVVEKSGETDWKQVEPARGPTKDGRVANVLL
                     ALKSLKWKEIASKGGDDAKFGLDRPELEVSVFKADGTEVGTLLVGKTDGAVTYVKVKA
                     EPGIFAVSSKDLEDLRKARTDIPV"
     gene            complement(3167..4747)
                     /locus_tag="VNU03_04130"
     CDS             complement(3167..4747)
                     /locus_tag="VNU03_04130"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HWP73671.1"
                     /translation="MTSLLKRQAITGAVVCFLIAAALGFFFPLPAQWVFLGLGVVLLL
                     LSLVLNAREARTAMGTRTARYGAGAALMVLLALGIVVAANAISVRHNTRWDLTENKRN
                     SVSPQTIQVLRTLKSPVTAIAFYRSDTPGKKTAEDLLAQYKTYSGGKFEWRLEDTDKA
                     PALARQYGVESYGTVVLEGGPAGQLRTEKVLDADEEKLTNGIVKVTRAGKRVVYIVKG
                     HGEREIGSADRGGFSQAKEQMEKANYEVKELFLARDPKIPDDAAIVMIPGPKTDLFPQ
                     ELAAIDAYVARAGKVFFMAVPFQATPTTKYLSKYGITVDDDVVIELNPIGQVFGVGPL
                     VPVVGQYDPHPITKDMGGVMTLFPLTRSVEPAKTLPKGVQASPLAQTSRQSWGETDKN
                     VFQTGKATPDPGEKTGPLPVAVVATVDAEPPAKAEGGEAPKKAAKARIVVVGTADFAS
                     NQFLGAQGNRDFFLNVVSWLAEEEDLISIRAKDPKQNPVVLTSAQSRLVWGLPLVGLP
                     GAVLICGLVVIAQRRRAR"
     gene            complement(4749..5516)
                     /locus_tag="VNU03_04135"
     CDS             complement(4749..5516)
                     /locus_tag="VNU03_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP73672.1"
                     /translation="MRWLPVFKKEMQLYFGSPVAYVVFTFFLFITGIFFGSIFSFYSD
                     ASMRSFMQPQFGQNLSVIENVMRPLFSNMSVVLLFFIPMLTMRLFAEERKSGTFELLL
                     TYPVRDGEILAGKYLAALALYVVLLALTLLYPGLVAYFTRVEWGPILTGYLGLVLTGA
                     AFLAVGVLVSSLTENQIVAGFGTFGILLAFWIVGWGAEFAGGGLRGVLQYLSFTDHLD
                     GFTRGLIDTKDLVYYLTGIVLALFLTLRSLESKRWRG"
BASE COUNT          832 a         1980 c         1943 g          885 t
ORIGIN      
        1 gccgccgcgc tggctccgcg catccaggtc aacgcggtgg ctccgggcgt ggtcctgctg
       61 ccggccggct tcccgcgcga gcgcggccga cgcctggccg cgcggatccc gatggggcgc
      121 cccggccggc ccgcggacgt ggcggaggcg gtgcggttct tcgccacgtg ctccgactac
      181 gtgaccgggc aggtgctctt cgtcgacggc gggatgtcgg tgcgctgagc cgcggagcgg
      241 ggctaggtgc ggccggcccg gccgccgccc atggctccca ggaactcgct catggacgcg
      301 aagcgcatca tcgggttctc gcgcttctcg gccccgatgg tggtctggga tcccccgtag
      361 ttgtggccgg gcagcaccac cgtgtcgtcg gggagcgcgc cgagccgctg ggtgagcgag
      421 gtgtacatct ccttcggatc gctgccgggc agatcggtgc gcccgcagga gcggatgaag
      481 agggtgtcgc ccgagatgag acagccgtcc accaggaagc actgcgagcc cggggtgtgc
      541 cccggcgtgt gcacgaacgt gagcgggatc gcgcccacct gcagcgtgtc gcccgcttcc
      601 accttcacca ggtccgagcc gaagcccttc aggaactcgc gctcggcgcg gtgcacgtag
      661 accttcgcgg ggtgcttgcc cagcagctcc gggatgccgg gaatgtcgtg accgaagagg
      721 tgcccgccca cgtggtcctg gtgggtgtgg gtcaccagca cgccggtgag ccgcatgtcg
      781 tcggcggcca ccagatcggc gatgccgtcg atctcccacg cgggatccac caccacgcac
      841 tcacgcgcgg atcgatcgcc gatcagatac acgaagttct gcatcgggcc cacctcgacc
      901 tgcttgaggt agaggcctgc cggcatcagt acccgggcac ccagaccggc tggtagtatc
      961 cgctgctcac gagctggggc tggtagcccc ccgcgaccat gatgccgtcc gcggtgacgt
     1021 accccgggac ccacacgtac gtggtggcaa cggtcgggac ccactgatac gcccagtagc
     1081 cggtcgccgg ggccggcgcg cagcatggat agctgacggc cggcggcggg cggtgatgcc
     1141 ccggcggggg ccccactggc acctgggacg cgggccgggg cgcgagcgga gtcggcgggg
     1201 gcgtcatggc gaaggggttg ggctggaaat cggtgggctt cagcacctca ccggccgaca
     1261 cgaccccggc cgtggagccg gtcaccgcga ccacgaatcc gatggcgacc accagtgcct
     1321 tcatgtcccc tccgacgccg ggcgtgagac ctgtattcgc ccctccattc tgccaccgaa
     1381 gcggccccga tcagaccggg atgtcggtgc gggcttttct caggtcttcc aggtctttgg
     1441 aggacaccgc gaagataccg ggctcggcct tgaccttcac ataggtgacc gccccgtcgg
     1501 tcttgccgac gagcagcgtg ccgacctcgg taccgtcggc cttgaagacg ctcacctcga
     1561 gctcgggccg gtccaggccg aacttggcgt cgtcgccacc cttcgaggcg atctccttcc
     1621 acttgagcga cttgagcgcg agcagcacgt tggcgacgcg cccgtccttg gtcgggccgc
     1681 gcgccggctc gacctgcttc cagtcggtct cgccgctctt ctcgacgacc agcggcttgt
     1741 cgccgctcac ccgcgcgcgc ttcacgtcac cgaggtcgaa cgtggggaac acggtccgat
     1801 cccgcagctg ggccacgctc ggggtcagct cggtgagggc cttgccctcc accagcacca
     1861 ccggtccctc gccctgtacc gcggcgagcg cggcgggctg accgccgcgg cgctcgttgg
     1921 aggactggag caggagcgtc ttcggctcct tcgcccccgt ctcccacagc cgcacgatca
     1981 cctccggctt cgagaggaag cgcggcacct cggccgctcc ctcggacagg aagcccagcg
     2041 cctgcaggtc ccggatcttc cacaagatct gggtgaccgc gccgctgtcg gcgcgcaggg
     2101 cctccggcgc ggtgatcttc cagcccgtgc cttccttcgc gagcgcgagg gtctcccgcc
     2161 cgcgcgtgat ctcgatcctg gcgagcttgt cggaggcgta gctcaccacc gtcttgtcgc
     2221 ggatcgcgcc caccgtcttg gggaagaccg tccacaccgc ctccgcggtc aggatgacgc
     2281 cgggctcccc ctcgcgcttg acgtagacgc ccttcttctc cgggaccgga cggccaacca
     2341 gcagggtccg ggccgcgcga tccttgtcct tgccgaccca gaccgtgacc ttcgagggct
     2401 tgtcgaggcc gtagggctgg agcgacttcg gggcgtcgtc gacgaactcc accgccttcg
     2461 cgccctcgag cttgtcgagg aactcggaga tcaggtccgt gtcggcccgc agcgcacgcg
     2521 gcttgacgat ccgccacttg ccgggctcgt cggcgaccac cccgatctgg tcgccgttga
     2581 cgtcgaggtc gatgccgctc acgttgcgcc ggtcgaaggc gatgacggtg cggtcgcgga
     2641 agtcggccag cggccgggtc gcgtcgcgcg cgatgctctc ggagagcgtg atgaccgcgg
     2701 gcttgccgcc ttcgcgcgcg tagacccaca ccccggtcgg gttcttgctg cccacccgca
     2761 ggaccagcgg ctcggtccgg cccttgacct cgagcttgac ctccgcctcc gggggatcga
     2821 ggccgaagtc ggcgggcttc gacgggttgg cgtcgatctc ccggtccatg cgcgcggtgg
     2881 cgaggccggt caccatgtcg tcgaccgccc cgcgatcgcc ccgcgtcttg atcggctcga
     2941 gcatctccca gctcccgtcg gcggtgcgct ggagccgtat cgtctcgccc ttgcgcgcga
     3001 cggtgacccg ctcgacgtcc ttcggctcga cggtccagat ccggcccttg gccgactccg
     3061 ccttctcccg ccggggcgcg agggagtagg tgtcgtagta gaagaagccg ccgagcccgg
     3121 cggccaggat cagcaggacg aggatcgtcc gcccgcgaga catgcgctag cgcgcgcggc
     3181 ggcgctgggc gatcaccacg agaccgcaga tcagcacggc gccgggcagc cccaccaggg
     3241 gcaggcccca taccagccgg gattgcgcgg acgtgagcac caccgggttc tgcttgggat
     3301 ccttggcccg gatggagatg aggtcctcct cctccgcgag ccaggagacc acgttcagga
     3361 agaagtcccg gttgccctgg gcgcccagga actggttcga cgcgaagtcg gcggtgccca
     3421 ccaccacgat gcgagccttc gccgccttct tgggcgcctc gccgccctcg gccttggcgg
     3481 gcggctccgc gtccaccgtc gccaccaccg ccaccggaag cgggccggtc ttctcgccgg
     3541 ggtccggagt ggccttgccg gtctggaaga cgttcttgtc ggtctctccc cacgactggc
     3601 ggctcgtctg ggcgagcggc gaggcctgga cgcccttggg gagagtcttg gccggctcga
     3661 cggagcgggt cagcgggaac agggtcatga ccccgcccat gtccttggtg atcgggtgcg
     3721 gatcgtactg gcccaccacc gggaccagcg ggcccactcc gaagacctgg ccgatcgggt
     3781 tcagctcgat gaccacgtcg tcgtcgacgg tgatgccgta cttgctcagg tacttcgtgg
     3841 tcggggtggc ctggaagggc accgccatga agaagacctt gcccgcccgc gcgacgtagg
     3901 cgtcgatcgc ggccagctcc tgcgggaaga gatcggtctt ggggcccggg atcatcacga
     3961 tggccgcgtc gtcggggatc ttggggtcgc gggccaggaa cagctccttg acctcgtagt
     4021 tggccttctc catctgctcc ttggcctggc tgaagccgcc ccgatcggcg gagccgatct
     4081 cccgctcgcc gtgtcccttc acgatgtaga ccacccgctt gcccgcgcgg gtgaccttga
     4141 cgatcccgtt ggtcagcttc tcctcgtcgg cgtcgagcac cttctcggtc cggagctggc
     4201 cggcggggcc accctccagc accacggtgc cgtagctctc gaccccgtac tggcgcgcga
     4261 gggcgggggc cttgtcggtg tcttccagcc gccactcgaa cttgcccccc gagtacgtct
     4321 tgtactgcgc gagcaggtcc tccgcggtct tcttcccggg agtgtcggaa cgatagaacg
     4381 cgatcgcggt caccggcgac ttgagcgtgc gcagcacctg gatggtctgg ggcgagacgc
     4441 tgttgcgctt gttctcggtg aggtcccagc gggtgttgtg ccggaccgag atcgcgttgg
     4501 ccgccaccac gatgccgagc gcgagcagga ccatgagcgc ggcgccggcc ccgtagcgcg
     4561 cggtgcgggt gcccatggcg gtgcgggcct cgcgcgcgtt cagcacgagc gagagcagca
     4621 gcaggaccac gcccagcccc aggaaaaccc actgggcggg caacgggaag aagaacccga
     4681 gcgcggccgc gatcaggaag cacaccactg ctccggtgat cgcctggcgc ttgagcaggc
     4741 tcgtcacgct atcccctcca ccgcttcgac tcgagggacc ggagggtgag gaacagcgcg
     4801 agcacgatgc cggtcaggta gtagacgaga tccttggtgt cgatcagccc gcgcgtgaag
     4861 ccgtcgaggt gatcggtgaa cgacaggtac tggagcaccc cgcgcaggcc gccccccgcg
     4921 aactcggcac cccaccccac gatccagaac gcgagcagga tcccgaaggt gccgaagccg
     4981 gccacgatct ggttctcggt cagggacgac acgagcactc ccaccgccag gaacgcggcg
     5041 ccggtcagga ccaggccgag gtagccggtg aggatcgggc cccactcgac gcgggtgaag
     5101 taggcgacga gccccgggta gagcagggtc agggcgagca ggaccacgta gagggcgagc
     5161 gcggccaggt acttgccggc gaggatctcc ccgtcgcgca ccgggtaggt cagcagcagc
     5221 tcgaaggtcc ccgacttccg ctcctccgcg aagaggcgca tcgtgagcat cgggatgaag
     5281 aacagcagca ccacgctcat gttgctgaag agcgggcgca tcacgttctc gatcacgctg
     5341 aggttctggc cgaactgcgg ctgcatgaac gagcgcatcg acgcgtcgct gtagaacgag
     5401 aagatcgagc cgaagaagat gccggtgatg aacaggaaga acgtgaagac cacgtaggcc
     5461 accggggagc cgaagtacag ctgcatctcc ttcttgaaga cgggcagcca gcgcatcagc
     5521 gggcctcctc ctcggcggcc accacgtcgg gcgcgacctg ctcgcccgcg accacccgga
     5581 tgaagacctc ctcgaggctc atgcccacct ggtgcagctc ctggagggcc cagttcttgc
//
LOCUS       DATMCV010000174         2587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_410010, whole genome shotgun sequence.
ACCESSION   DATMCV010000174 DATMCV010000000
VERSION     DATMCV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410010"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="VNU03_04140"
     CDS             <1..1976
                     /locus_tag="VNU03_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWP73673.1"
                     /translation="MRERFTAAVATHKAGDWSAAAKEFGDPVWSGTPLEDYALLYQAD
                     SLLRQGDAVAARALAAQAADRSPESGLTASALIWAASVETLAGDPAAAVKVLQRVLTS
                     YPDRPDVPRTRYLLGEALRAAGDLPEAAKVYASLWLQAPASYGDAADAQLKALAAAGV
                     TTPAPTPGERALRAERLLSSGLIERARGEAEALVAEKPAPDVRERALRVVMNTSRRLG
                     RDEAAIAAAIDALAVVPADRRPGWLLELGRLRQRRDRETALATLDQLVREHPKSPDAA
                     DALLLKAELLEAANRLADAEKTYVRLAADYPDEDEAATALWRLGWAAWFRGNLIESTS
                     RWTRLQTARGGASLRESATYWLGRAWERRGDREQAARQFGTLVRDAPRSYYGLLAAKR
                     APGAGAPAGASSFAFPADPLEALQGDARFARAAALRGVGLREFADEELDDLTRRAVGE
                     PRRLYALSAAYVADERYHMALRILRRSFLGVARSGTGSPREFWEMFYPLGWRDALTAA
                     AGRAAVDPFLVAAVVREESSFYPAARSRVGARGLMQLMPDTGRAVAQSRQIPFPDADV
                     LDQPVTNLELGSTFFAGLLREFGDPRLAAAAYNAGPTRVREWWAGRRSDDLEVWVDQI
                     PYNETRAFVKRVMLSYEEYRRIYAEKPKGGGES"
     gene            1973..2434
                     /locus_tag="VNU03_04145"
     CDS             1973..2434
                     /locus_tag="VNU03_04145"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWP73674.1"
                     /translation="MSEGRPAALWRMAAAAAADALVGVVAWTLAAMWLMIALLLVRRH
                     PDVLDVVILTAGVLLVGIALHVVYHTVLVGGCGQTLGKMLMGVAVVRRDGAPAGYGRA
                     LLRCVGGGLCLLTLGLGRLPALFTRERRGLPDLVAGTRPISHPLPSGKRAG"
BASE COUNT          296 a          960 c          987 g          344 t
ORIGIN      
        1 acatgcggga gcggttcacc gcggcggtgg ccacccacaa ggcgggcgac tggagcgcgg
       61 cggccaagga gttcggcgat ccggtgtggt cggggacgcc gctcgaggac tacgcgctcc
      121 tctaccaggc ggacagcttg ctgcgccagg gcgatgcggt cgcggcgcgg gcgctcgccg
      181 cgcaggccgc cgatcgcagc ccggagagcg ggctcaccgc ctcggctctg atctgggccg
      241 cctcggtcga gacattggcg ggtgacccgg ccgccgcggt gaaggtgctg caacgggtgc
      301 tcacgagcta tcccgaccgg ccggacgtcc cgcgcacgcg gtatctgctc ggcgaggcgc
      361 tgcgggcggc gggcgatctg ccggaagcgg ccaaggtcta cgcgtcgctc tggctacagg
      421 cccccgcctc ctacggcgac gcggccgacg cgcagctcaa ggcgctggcg gcggccgggg
      481 tgacgacacc cgcgccgacg cccggcgagc gcgccctccg cgcggagcgt ctgctctcga
      541 gcggcctcat cgagcgggcc cgcggcgagg cggaagcgct ggtggccgag aagcccgcgc
      601 ccgacgtccg cgagcgcgcc cttcgcgtgg tgatgaacac ctcgcgccgt ctcggccgcg
      661 acgaagccgc gatcgccgcc gccatcgatg ccctcgcggt ggtgcccgcc gaccgccgcc
      721 cgggctggct gctcgagctg gggcggctcc gccagcggcg cgaccgcgag acggcgctgg
      781 ccacgctcga ccagctggtc cgcgagcacc cgaagagccc cgacgccgcg gacgcgctcc
      841 tcctgaaggc ggagctgctc gaggccgcca accggctcgc cgatgccgag aagacctacg
      901 tgcggctggc cgccgactat ccggacgagg acgaggccgc caccgcgctc tggcgcctcg
      961 gctgggccgc gtggttccgc ggcaacctca tcgagagtac gtcgcgctgg acgcgcctgc
     1021 agaccgcacg cgggggcgcg agcctgcgcg agtcggcgac ctactggctc ggccgcgcgt
     1081 gggagcgccg cggcgaccgc gagcaggccg cgcggcagtt cggcacactc gtgcgcgacg
     1141 cgccgcggtc ttactatggc ctcctcgcgg ccaagcgcgc gccgggcgcg ggcgcgccgg
     1201 ccggcgcgtc gtccttcgcc ttccccgccg atccgctcga ggccctgcag ggagacgcgc
     1261 gcttcgcccg cgcggcggcg ctccgcgggg tcgggctgcg ggagttcgcc gacgaggagc
     1321 tcgacgatct cacccgccgc gcggtgggcg agccccgccg cctctacgcg ctctccgcgg
     1381 cctacgtggc cgacgagcgc tatcacatgg cgctgcgcat cctgcggcgg agcttcctcg
     1441 gcgtggcccg cagcggcacc ggctcgccgc gcgagttctg ggagatgttc tacccgctcg
     1501 gctggcgcga cgccctcacc gcggcggcgg ggcgcgccgc ggtcgacccg ttcctggtcg
     1561 cggcggtggt gcgcgaggag tcgtcgttct atcccgcggc ccgatcgcgg gtgggcgccc
     1621 gcgggctcat gcagctcatg ccggacaccg gccgcgcggt ggcccagtcc cggcagatcc
     1681 cgtttcccga cgccgacgtg ctcgaccagc cggtcaccaa cctcgagctg ggcagcacct
     1741 tcttcgcggg cctgctgcgc gagttcggcg acccgcggct ggccgccgcg gcctacaacg
     1801 ccgggcccac ccgggtgcgc gaatggtggg ccgggcgtcg ctccgacgat ctcgaggtct
     1861 gggtggatca gatcccctac aacgagacgc gggccttcgt gaagcgcgtg atgctctcgt
     1921 acgaggagta tcggcgcatt tacgcggaga agccgaaggg cgggggcgag tcctgagcga
     1981 ggggcgcccg gccgcgctct ggcgcatggc ggccgccgcg gccgccgatg cgctggtggg
     2041 cgtggtggcc tggaccctcg ccgcgatgtg gctgatgatc gcgctgctcc tggtccgtcg
     2101 ccacccggac gtcctggacg tggtgatcct caccgcgggc gtgctgctgg tggggatcgc
     2161 gctccacgtc gtctatcaca ccgtgctggt gggcggctgc gggcagaccc tggggaagat
     2221 gctgatgggc gtcgcggtgg tgcgccgcga cggtgcgccc gccggctacg gtcgcgccct
     2281 gctgcgctgc gtgggcgggg ggctgtgcct gctgactctc gggctcggcc ggctgcccgc
     2341 gctcttcacc cgcgagcgtc gcgggctccc cgacctggtc gcgggcaccc gccccatctc
     2401 ccaccctctc ccctccggga agagggcagg gtgaggggtc gaatatgagg gatcgagggg
     2461 caacctatcg cggcgggcgc acccgcgcga cgggattgac cggcttcgcc ggcccttcga
     2521 gcggctcgag cgcggcgagc cgctcgtcga gcgagggatg cgtggcgagc agcccctcga
     2581 gcccctc
//
LOCUS       DATMCV010000175         7375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_875352, whole genome shotgun sequence.
ACCESSION   DATMCV010000175 DATMCV010000000
VERSION     DATMCV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875352"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1148
                     /gene="tssG"
                     /locus_tag="VNU03_04150"
     CDS             39..1148
                     /gene="tssG"
                     /locus_tag="VNU03_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HWP73675.1"
                     /translation="MAPESRRTDPSVEELLFDEGFRFEFFQAVRLLEKLFPERGPVGR
                     DARPGEEVVRFLAHNSLTFPPSSIHDLSRSEDGGPASMVVAFMGLTGPSGVLPRHYTE
                     LVLEQERRRERGLASFFDLFNHRMISLFYRAWQKYRVPIAHEQASRPGAPPDPFTESL
                     YAHFGMATGGLRGRQQVEDQSFVFYAGLLAQQPRSASALEGMITDYFSVPTKVRQFDG
                     QWLPLVEENRSRVGRAGRPGEDDRGAHNVLGRTAVLGKRFWDQQAGFRLRLGPMGYAE
                     FLELLPMGARFPVLVQMVRFFVSQGLDFDVQLVLKGDDVPVCRLGDQGPGAPRLGWSS
                     WLGGEGRTRDVDDAVLGRRWTRGALPDRESVRRAA"
     gene            1145..3817
                     /gene="tssH"
                     /locus_tag="VNU03_04155"
     CDS             1145..3817
                     /gene="tssH"
                     /locus_tag="VNU03_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HWP73676.1"
                     /translation="MNVTLKALVAKLNDTCRAALEGAAGLCLTRTHYDVDVEHFLVKL
                     CEAPNTDTTRIIKHYGVDPGKLAAGLTRALDRLKTGNARTPTLAPRITRWLSEAWTLA
                     SVELGASSLRSGHLLLALLADPDLARLAAESSPELAKISVEDLTKRFGELTAGSVEDA
                     YQAPSVVDGAPARGAGTGKTPALDQYTVDLTARARQGEIDPVLGRDFEVRQLIDILTR
                     RRQNNPILTGEAGVGKTAVVEGLALRIAAGDVPPPLRPVALRTLDLALLQAGAGVRGE
                     FENRLKSVIAEVKASPQPVILFIDEAHTMIGAGGQAGQGDAANLLKPALARGELRTIA
                     ATTWSEYKKYFEKDPALARRFQVVKVDEPGEAQAVAMMRGLVAMLEQHHNVRILDEAV
                     AEAVRLSNRYISGRQLPDKAVSVLDTACARVALGQVATPPALEDCQRLIAQIETEIAI
                     LEREAVTGGESRERRDELSKQNADTTARLSELEARWQQERRLIEELRGIRTKLEAHAT
                     AVKAAAASPPVEGRLSDEELATLQADLSRLTKELATVQGEAPLMQVCVDGQAVAEVVS
                     GWTGIPVGKMVKDEIQTVLSIRDLMERRIIGQSHALEAVSRRIQTARAKLEDPRRPQA
                     VFLLVGPSGVGKTETAHTLADLLYGGDRNMVVINMSEYQEGHTVSGLKGSPPGYVGYG
                     EGGVLTEAVRRRPYSVVLLDEIEKAHSDVIELFYQVFDKGSLEDGEGREIDFKNTVII
                     MTSNVGTDQMMKLCADPAHPPDPDELIEKLRPTLLRAFKPAFLGRAIVVPYYPIGDEA
                     MRRIIELQLGRIRTRLQENNRVRFTYDDALVAEIARRCTEVESGARNVDHILTRTLLP
                     EISREYLSRMASEASISRVHVSVDDGGRFGYQVD"
     gene            3817..5733
                     /gene="tssI"
                     /locus_tag="VNU03_04160"
     CDS             3817..5733
                     /gene="tssI"
                     /locus_tag="VNU03_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HWP73677.1"
                     /translation="MANSIQANRPISVTTPLGPDVLLLTGVRGEEGLSRPFRFELDLI
                     AEDPQKVAFDKLLGARVSVRLRLDDDRERHWSGICNRFSEVGQDVEFTHYHLEMVPQL
                     WLLTRRAQSRIFQNLSVPDILRKVLTGLDVTWEIQGTFEPRDYCVQYRETDFNFASRL
                     MEEEGIYYFFKHSSDNHRMVAANTPQSHPDMPLGSRIIFDETARDREDDLRVTSWHKS
                     QLLQSGKVTLWDHSFELPHKHLEAVEIIQDSVAVGKVTHKLKVGNNDQLELYDFPGEY
                     AQRFDGVDRGGGDRAGDLQKIFQDNGRTAKIRIQEEAAAGLIVSGSGQCRNFVSGHKF
                     TLERHHDGDGAYVLTSVSHRIAMASGDYRSGQASQFEYSNAFTCIPFALPFRPARTTP
                     RPVVPGPQTAVVVGPPGEEIFPDKYGRVKVQFHWDREGKADAASSCWIRVSQSHAGKG
                     WGSIFIPRIGQEVLVAFEEGDPDQPVIVGSLYNAQEMPPYSLPEEKTKTVLFKSNSST
                     GGGGHNEIRFEDSAGNEQIYVHGEKDLHVRIKDAVREWIGGDQDRIVKGTQNEEVEKN
                     WYQKTGMSIVIESGMELTIKSSGGFIKIDPSGITIMGTMVRINSGGAPGVIPKAPAEA
                     DDSESGAVDPPLSS"
     gene            5748..6041
                     /locus_tag="VNU03_04165"
     CDS             5748..6041
                     /locus_tag="VNU03_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73678.1"
                     /translation="MTDLTTHGSPLSPGPGSPDTIIGGLPAWRTIVDQHACPAASISG
                     ADGVGFVIVGCPTVLINTQMACRIQDIVVEKPGSAMGPVNPIAMGCPTVVIGE"
     gene            complement(6084..>7375)
                     /locus_tag="VNU03_04170"
     CDS             complement(6084..>7375)
                     /locus_tag="VNU03_04170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP73679.1"
                     /translation="TAVTSGAAAISALGLRNVVLHHLDVMDVSDAFGTFDYIIAPGLY
                     SWVPEPVRDRILDICDAHLAPHGVAFVSYNTYPGCHLRQAVWQILQFHVEGITDPRER
                     IAQACALARFMADCPQAEGSDGAVLGNEMRAVLDHSPGLLFHDDLAVTNDPVYFREFA
                     SHARRHRLQFLAEANVHEMQPQAYPPEIARQVWHLANESVVQKEQYLDFLRNRRFRQT
                     LLCREDVPLDRLLQPEVLRGLHFGFPARPFAERPDVNSPGVVVEFRGRGDSTLSTDHP
                     LAKAAMHHLGQVWPRLVGFDDLLRAIGRSGASAADGGALRDILLAAYNAGLVELRTLA
                     PRFTLEPGPRPIASPVARWQLEHGGFVASLRHTSVWIEDDLGRRLLTLMDGTRTAEML
                     RQELGAYVSAKASPVQVSAADVDRKIVEAARLALLIA"
BASE COUNT         1235 a         2438 c         2620 g         1082 t
ORIGIN      
        1 gtcgcgcgga cccgtcagcg caaagagccc ctcaagcgat ggcccccgag agccggcgaa
       61 cagatccttc tgtagaggag ctcctcttcg acgaggggtt ccggttcgag ttcttccagg
      121 cggttcgact gctcgagaag ctgttccccg aacggggccc ggtggggcgc gacgcccgcc
      181 ctggcgagga ggttgttcgg ttcctcgccc acaattcgct gacctttcct cccagctcga
      241 tccacgatct ctcccgctcg gaggatgggg ggcccgcctc gatggtggtg gccttcatgg
      301 ggttgaccgg gccctcgggc gtgttgccca gacactacac cgagctggtg ctcgagcagg
      361 agcggcgccg cgagcgtgga ctcgcgagct tcttcgacct gttcaatcat cggatgatct
      421 cgctgttcta tcgggcgtgg cagaagtacc gcgtgccgat cgcgcacgag caggcgagcc
      481 ggccgggggc gccgccggac ccgttcaccg aatcgctgta tgcccacttc ggcatggcga
      541 ccggcggcct ccggggccgg cagcaggtgg aggatcagag cttcgtcttc tatgccgggc
      601 tcctcgccca gcagccgcgc tccgcgagcg cgctcgaggg gatgatcacc gactacttct
      661 cggtgccgac gaaggtccgg cagttcgacg gacagtggct gccgctggtg gaagagaacc
      721 ggtcccgcgt cgggcgcgcc ggccggcccg gcgaggacga ccggggggcc cacaacgtgc
      781 tggggcgcac cgcggtgctt ggcaagcgct tctgggacca gcaggcgggg ttccggctgc
      841 gcctgggccc catgggctat gcggagttcc tcgagctcct tccgatgggt gctcgcttcc
      901 cggttctcgt gcagatggtc cgcttcttcg tgagccaggg gctcgatttc gacgtgcagc
      961 tcgtgctgaa gggcgacgat gttccggtgt gccggctggg agaccagggg cccggcgcgc
     1021 cccgcctcgg ctggtcctcc tggctcgggg gagaagggcg cacgcgggat gtggacgacg
     1081 ccgtgctggg ccgccgctgg acgcgtggcg cgctacccga tcgtgaatcc gtgaggagag
     1141 ccgcatgaac gtcaccctga aagctctggt cgccaagctc aacgacacgt gccgcgccgc
     1201 cctggagggc gccgccggcc tgtgtctgac gcggacccac tacgacgtcg acgtcgagca
     1261 tttcctggtg aagctctgcg aggcgccgaa cacggacacc acgcgtatta tcaagcacta
     1321 cggcgtggat cccggcaagc tcgcggcggg cctcacgcgg gcgctcgacc ggctgaagac
     1381 cggcaacgcc cggaccccca cgctcgcgcc gcggatcacg cgctggctga gcgaggcgtg
     1441 gacgctggcg tccgtcgagc tgggcgcgtc gtcgctgcgc tccggtcatc tcctcctggc
     1501 gctgctcgcg gatcccgacc tcgcccggct ggccgcggag tcgagccccg agctcgcgaa
     1561 gatctccgtc gaggacctga ccaagcgctt cggggagctg accgcggggt cggtcgagga
     1621 cgcgtaccag gcgcccagcg tggtggacgg agcgccggcc aggggcgccg ggaccggcaa
     1681 gacgcccgcg ctcgaccagt acaccgtcga tctcacggcc cgcgcccgcc agggcgagat
     1741 cgatccggtc ctcggacggg acttcgaggt ccggcaactc atcgacatcc tcacgcgccg
     1801 gcggcagaac aatcccatcc tcaccggcga ggccggggtc ggaaagaccg cggtggtgga
     1861 ggggctcgcg ctccggatcg cggccggcga cgtgccgccc ccgctgcgtc cggtcgcgct
     1921 acgcaccctg gacctggcgc tcctccaggc cggcgcgggc gtgcggggcg agttcgagaa
     1981 ccgcctcaag tccgtcatcg ccgaggtcaa ggcctcgccg cagccggtga tcctgttcat
     2041 cgacgaggcg cataccatga tcggcgccgg cgggcaggcc gggcagggcg atgcggccaa
     2101 cctgctcaag ccggccctgg cgcgcggtga gctgcgcacg atcgcggcca cgacgtggtc
     2161 ggagtacaag aagtacttcg agaaggatcc cgcgctcgcc cggcggttcc aggtcgtaaa
     2221 ggtggacgag ccgggcgaag cgcaggcggt ggccatgatg cgcgggctcg tcgccatgct
     2281 cgagcagcat cacaacgtcc gcatcctcga cgaggcggtc gcggaggcgg tgcggctgtc
     2341 gaaccgctac atctcgggcc ggcagctgcc tgacaaggcg gtgagcgtgc tcgacaccgc
     2401 gtgcgcgcgg gtcgccctcg gacaggtggc gacgccgccg gccctcgagg attgccagcg
     2461 cctgatcgcc cagatcgaga ccgagatcgc cattctcgag cgcgaggcgg tcaccggagg
     2521 ggagagccgg gagcggcggg acgagctgtc caagcagaac gccgacacga cggcgcgcct
     2581 ctcggagctg gaggcgcgat ggcaacagga gcggcgcctg atcgaggagc tgcggggcat
     2641 ccggaccaag ctcgaggccc acgcgacggc ggtgaaggcg gccgcggcct cgcccccggt
     2701 cgagggacgt ctgtccgacg aggagctggc cacgctgcag gccgacctgt cgcggctcac
     2761 caaggagctg gccaccgtcc agggcgaggc gccgcttatg caggtgtgcg tcgacggcca
     2821 ggcggtggcg gaggtcgtct ccggctggac cggcatcccg gtcggcaaga tggtcaagga
     2881 cgagatccag accgtgctct cgatccggga cctgatggag cgacggatca tcggccagtc
     2941 ccacgccctg gaggcggtca gccggcgcat ccagaccgcg cgggccaagc tggaggatcc
     3001 ccggcgtccc caggcggtgt tcctcctggt ggggccgagc ggggtgggca agaccgagac
     3061 cgcccatacc ctggccgacc tcctgtacgg cggcgatcgc aacatggtcg tcatcaacat
     3121 gtccgagtac caggaaggtc acaccgtctc ggggctcaag gggtctcccc ccggctacgt
     3181 ggggtacggc gaggggggcg tgctcaccga ggcggtccgg cgtcggccgt acagcgtcgt
     3241 cctgctcgac gagatcgaga aggcccactc ggacgtgatc gagctcttct atcaggtctt
     3301 cgacaagggc tcgctggagg acggggaagg gcgagagatc gacttcaaga acaccgtcat
     3361 catcatgacc tccaacgtgg gcaccgacca gatgatgaag ctgtgcgcgg atccggccca
     3421 tccgccggat ccggacgagc tgatcgagaa gcttcggccc accttgctgc gcgccttcaa
     3481 gccggcgttc ctcggccggg ccatcgtcgt cccgtactac ccgatcggcg acgaggcgat
     3541 gcggcgcatc atcgagctcc agctcggccg gatccgtacc cggctgcagg agaacaatcg
     3601 ggtgcgattc acctacgacg acgcccttgt cgcggagatc gcgcggcggt gcacggaggt
     3661 ggagtcgggg gcgcgcaacg tcgaccacat tctgacccgc accctgctgc ccgagatctc
     3721 ccgggagtac ctctcgcgca tggcctcgga ggccagcatc tcccgggtgc acgtgagcgt
     3781 ggacgacggc ggccgcttcg gctaccaggt cgactgatgg cgaactccat ccaggcgaac
     3841 cggccgatct cggtcaccac cccgctgggg ccggacgtct tgctgctcac cggggtgcgg
     3901 ggcgaggagg gcctgtcacg gccattccga ttcgagctgg acctcatcgc cgaggacccg
     3961 cagaaggtcg ccttcgacaa gctgctcggc gcccgggtca gcgtccgcct caggctcgat
     4021 gacgaccgcg agcgccactg gagcgggatc tgcaatcggt tcagcgaagt cgggcaagac
     4081 gtcgagttca cgcactacca cctggagatg gtcccgcagc tctggctcct gacccggcgg
     4141 gcccagagcc ggatcttcca gaacctgagc gtcccggaca ttctccgcaa ggtcctgacc
     4201 ggcctcgacg tgacgtggga gatccagggc acgttcgagc cgcgcgacta ctgcgtgcag
     4261 taccgggaaa ccgacttcaa cttcgcctcc cgcctgatgg aagaggaagg catctactac
     4321 ttcttcaagc actcgtccga caaccaccgg atggtggcgg ccaatacccc gcagagccat
     4381 cccgacatgc ccctggggag ccggatcatc ttcgacgaga cggcccggga ccgggaggac
     4441 gatctgcggg tcacgagctg gcacaagagc cagttgctgc agtcagggaa ggtcacgctg
     4501 tgggatcact ccttcgagct gccccacaag catctggaag cggtggagat catccaggac
     4561 agtgtggcgg tgggcaaggt gacccacaag ctgaaggtcg ggaacaacga ccagctcgag
     4621 ctgtacgact tcccgggcga gtatgcccag cggttcgacg gcgtggaccg cgggggaggg
     4681 gatcgcgccg gtgatctgca gaagatcttc caggacaacg gacggaccgc gaagatccgg
     4741 atccaggagg aggcggcggc cggcctcatc gtctccgggt ccgggcagtg tcggaacttc
     4801 gtgagcggtc acaagtttac gttggagcgg caccatgacg gcgacggcgc ctacgtgctg
     4861 acgagcgtca gccaccggat cgcgatggcg agcggcgact atcgctccgg gcaggcgagc
     4921 cagttcgagt acagcaacgc gttcacctgt attcccttcg cgctgccctt ccggcccgcg
     4981 cggaccaccc cgaggccggt cgttcccgga ccacagaccg ccgtggtggt gggtccgccc
     5041 ggcgaggaga tcttccccga caagtacggg cgggtgaagg tccagttcca ctgggaccgg
     5101 gagggcaagg ccgacgcggc gagctcctgc tggattcgcg tctcccagag ccacgccggg
     5161 aagggctggg gctcgatctt catcccccgg atcggtcaag aggtcctggt cgcgttcgag
     5221 gagggcgacc cggaccagcc cgtgatcgtc gggtccctct acaacgccca ggagatgccg
     5281 ccctattcct tgcccgagga gaagaccaag accgtcctct tcaaatcgaa cagcagcacg
     5341 gggggcggcg gccacaacga gatccgattc gaggatagcg cgggcaacga gcaaatctac
     5401 gtccacgggg agaaggatct ccacgtccgg atcaaggatg cggtacgcga gtggatcggc
     5461 ggcgatcagg atcgcatcgt caaggggacc cagaatgaag aggtcgagaa gaactggtac
     5521 cagaagacgg gcatgagcat cgtcatcgag tccggcatgg agctgaccat caagtcgtcc
     5581 ggcggcttca tcaagatcga tcccagcggt atcacgataa tggggaccat ggtccggatc
     5641 aacagcggcg gggcgccggg cgtcattccg aaggctccgg cggaggcgga cgacagcgag
     5701 tcgggcgccg tcgatccgcc cctaagctca tgaagcttgc cgcccgagtg acggatctca
     5761 ccacccacgg gagtccgctc tccccggggc cgggcagtcc cgacacgatc atcggcgggc
     5821 tgcccgcctg gcgaacgatc gtggatcagc acgcctgtcc ggcggccagc atcagcgggg
     5881 ccgacggagt gggcttcgtg atcgtgggct gcccgaccgt actgatcaac acgcagatgg
     5941 cgtgccgcat ccaggacatc gtggtggaga agccgggctc cgcgatgggc ccggtgaacc
     6001 ccatcgcgat ggggtgcccc accgtggtga tcggcgagta gggctgtcga cgctccccgc
     6061 gactcgtgac gctgtagcag ccgttacgcg atgagcaggg cgagccgcgc cgcctcgacg
     6121 atctttcggt cgacgtcggc cgcgctgacc tggaccggag acgccttcgc ggagacgtat
     6181 gcccccagct cctggcgtag catctccgcg gtgcgggtgc catccatcag cgtcagcagt
     6241 cggcggccga gatcgtcctc gatccagacg ctcgtgtgac gcagggacgc cacgaacccg
     6301 ccgtgctcga gctgccaccg cgccaccgga ctcgcgatcg gccggggacc cggctccagc
     6361 gtgaagcgcg gggcgagggt gcgaagctcg acgaggccgg cgttgtacgc ggcgaggaga
     6421 atgtcgcgca gggcgccccc gtcggccgcg ctcgcgccgc ttcgaccgat ggcccgcagg
     6481 agatcgtcga agccgaccag acgcggccag acctggccga gatgatgcat cgcggccttc
     6541 gcgagcggat ggtcggtcga gagcgtggag tcgccacggc cgcggaactc cacgaccaca
     6601 cccggcgagt tcacgtccgg acgctcggcg aacggacggg cggggaaccc gaagtgcagg
     6661 ccgcgcagga cctccggctg gaggagacgg tcgaggggca catcctcccg acacaggagg
     6721 gtttgacgga agcggcgatt ccgcaggaaa tcgaggtact gctccttctg caccacgctc
     6781 tcgttggcca ggtgccagac ctggcgagcg atctcgggtg ggtacgcctg gggctgcatc
     6841 tcgtggacgt tggcctcggc caggaactgc aggcggtgcc ggcgggcgtg cgacgcgaac
     6901 tcgcgaaagt acaccggatc gttggtgacc gcgaggtcgt cgtggaagag aagccccggg
     6961 gagtggtcga gcaccgcgcg catctcgttg ccgagcaccg ccccgtcgct cccttcggcc
     7021 tgcgggcagt ccgccatgaa gcgcgcgagg gcgcaggcct gggcgatccg ctcgcgcgga
     7081 tcggtgatgc cctcgacgtg gaactggagg atctgccaga ccgcctgccg caggtggcag
     7141 ccgggatagg tgttgtagct aacgaacgcc acgccgtgcg gcgccaggtg ggcatcgcag
     7201 atgtcgagga tgcgatcgcg caccggctcg ggaacccacg agtagaggcc cggcgcgatg
     7261 atgtagtcga acgtaccgaa ggcgtcggac acgtccatga cgtcgaggtg gtgcaacacg
     7321 acgttgcgca ggccgagggc cgagatggcg gcggcgccgc tggtcaccgc ggtgg
//
LOCUS       DATMCV010000176         4457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_8987, whole genome shotgun sequence.
ACCESSION   DATMCV010000176 DATMCV010000000
VERSION     DATMCV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8987"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VNU03_04175"
     CDS             complement(<1..921)
                     /locus_tag="VNU03_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73680.1"
                     /translation="MATFNFELSTLPPEAEALRDEVRTFLKQELAQFQPADRARSWGG
                     FNRDFSRRMGERGWIGMTWPKKYGGHERSALERYVVLEETLAAGAPVSAHWVADRQSG
                     PLLLRFGTEEQRQRFLPRISRGELAFAIGMSEPDSGSDLASIRTRAEKVDGGYRVNGT
                     KIWTSNAHLSDFAIALFRTKVVPDKKHEGLSQFLVDLKSPGISIRPIIDLAGGHHFNE
                     VHFQDCLVPDDMLVGKDGEGWKQVTTELGFERSGPERYLSSLQLVIELIREAGTEPGE
                     RAAALIGHFTAHLVTLRQMSLSIATMLQAGQ"
     gene            complement(936..2099)
                     /locus_tag="VNU03_04180"
     CDS             complement(936..2099)
                     /locus_tag="VNU03_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73681.1"
                     /translation="MTVFPGFTLPEELRLLGDQIRRFIQDEIIPLEQTIDPDAPEIPR
                     EDFVRLSAKTKAAGLWTLGAREEYGGGGLDTFSMCVVLEAMAQHRMGLYNPGCGVFGR
                     YPPPAIWAGSKAQIEKYAVPALREGYETFFAITEPAGGSDPAGAIQTRAERRGDKWVL
                     NGRKVFISRAHDAPWGIVFARTDKGKGRGGISCFILEHGTPGFHAKPIRVIRTSAIPN
                     DVSFEDCEIPAENLIGQEGQGLDLAFDLLVKNRFPYSACNLGVATAAHRMAVAHAKQR
                     STFGAPLSQRQAIQWMLADAEVELRACRWLIWEGAWKADRGEDARVEASIAKLYSSEV
                     LGRVVDAAVQIHGGYGVSKEFPLERWYREARVRRIGEGPSEVHRMVIARSLFR"
     gene            complement(2509..2811)
                     /locus_tag="VNU03_04185"
     CDS             complement(2509..2811)
                     /locus_tag="VNU03_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73682.1"
                     /translation="MTWWHRAGGTTVAFSTAVETMMGSTLATQSPRPRGDAVALQTQL
                     GPTTDLAALVERVVRNRLPWVVVPAAALRAWEERDSDGWAKVSAWLASNDVVVVRI"
     gene            2999..3379
                     /locus_tag="VNU03_04190"
     CDS             2999..3379
                     /locus_tag="VNU03_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73683.1"
                     /translation="MNNQKWYGHKWCTVIATLCGVLAVAGPAGADCAWVLWSQVHDPK
                     PGGWVLQTAFPNVGACTKALDQREKEGRKAIYVTEGGAKVKGLTDRRASTDLLLLYGS
                     DASSGGVAWQCFPETVDPRGPKAK"
     gene            3462..>4457
                     /locus_tag="VNU03_04195"
     CDS             3462..>4457
                     /locus_tag="VNU03_04195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016828285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP73684.1"
                     /translation="MQKRWSLSVPMDGFALTELTEVAREAEQLGYTDAWSFEVDGIDG
                     FTPLAVIGLATNMRLGTAIANVYTRGPATLASTAAGLADLAPGRFVLGIGAGSQPIVE
                     SWNGGQFSRPATRVRETAQFLRKALAGERVVWKGETFSVDGFRLTRVPAHPVPIYVAA
                     LREGMLKVAGQVGDGVILNWLSPDDVPRSVGVVRAAARQAGRDPDAIEVTARLFVNLD
                     TPGPESDLAVRRHIAGYLNVPVYRAFQEWLGRTESLGPMWRAWGAGDRRGAVAAIPEQ
                     VMDDLMPRGSLADLKARIQLYLDRGIDTAFLSLMTTETDPIKKRDLMLNALRVLAP"
BASE COUNT          677 a         1582 c         1539 g          659 t
ORIGIN      
        1 ctgccctgcc tggagcatgg tggcgatcga gagcgacatc tggcgcagcg tcaccaggtg
       61 cgcggtgaag tggccgatca gcgccgccgc gcgctctccc ggctccgtgc ccgcctcgcg
      121 gatgagctcg atcacgagct ggagcgagga gaggtagcgc tcgggcccgc tgcgctcgaa
      181 cccgagctcg gtggtcacct gcttccagcc ctcgccgtcc ttgccgacca gcatgtcgtc
      241 gggcaccagg cagtcctgga agtggacctc gttgaagtgg tgaccgccgg ccaggtcgat
      301 gatcgggcgg atcgagatgc cgggcgactt gaggtcgacg aggaactggg acagcccctc
      361 gtgcttcttg tccggcacca ccttggtgcg gaacagcgcg atcgcgaagt cggagaggtg
      421 cgcgttgctg gtccagatct tggtgccgtt gacccggtag ccgccgtcca ctttctcggc
      481 ccgggtgcgg atcgaggcga ggtccgagcc cgagtccggc tcgctcatgc cgatggcgaa
      541 ggccagctca cctcgagaga ttcgcggaag aaagcgctgg cgctgctcct cggtgccgaa
      601 gcgcaggagc agcgggccgc tctggcggtc ggcgacccag tgcgcgctca ccggcgcgcc
      661 cgccgcgagc gtctcctcga gcaccacgta ccgctcgagc gcggagcgct cgtggccgcc
      721 gtacttcttc ggccacgtca tgccgatcca gccgcgctcg cccatgcggc gcgagaagtc
      781 gcggttgaag ccgccccagg agcgcgcgcg gtccgcgggc tggaactggg ccagctcctg
      841 cttgaggaag gtgcgaacct cgtcgcgcaa cgcctccgcc tcggggggaa gcgtcgatag
      901 ctcgaagttg aacgtcgcca tggtgggctc ctgatttacc ggaacagtga ccgcgcgatg
      961 accatccgat ggacttcgga cggtccctcg ccgatgcggc gcacccgcgc ctcgcggtac
     1021 cagcgctcca gcgggaattc tttcgacacc ccgtagccgc cgtggatctg caccgccgcg
     1081 tcgaccacgc ggcccagcac ctcgctcgag tagagcttgg cgatggaggc ctccacgcga
     1141 gcgtcctccc cgcggtcggc cttccacgcg ccctcccaga tgagccagcg gcacgcgcgc
     1201 aattccacct ccgcgtcggc cagcatccac tggatggcct ggcgctggct gagcggcgcc
     1261 ccgaaggtcg agcgctgctt ggcgtgggcg accgccatcc ggtgggccgc ggtggccacc
     1321 ccgagattgc aggccgagta cgggaagcgg ttcttgacca gcagatcgaa ggccaggtcg
     1381 agaccctggc cctcctggcc gatcaggttc tcggccggga tctcgcagtc ctcgaagctc
     1441 acgtcgttgg gaatcgccga ggtgcggatc acccggatcg gcttggcgtg aaagccgggc
     1501 gtgccgtgct ccaggatgaa gcacgagatg ccgccgcggc ccttcccctt atccgtccgc
     1561 gcgaacacga tgccccacgg cgcgtcgtgc gcgcgcgaga tgaagacctt ccgcccgttg
     1621 agcacccact tgtcgccgcg ccgctcggcc cgggtctgga tcgcgccggc cggatcggag
     1681 ccgcccgccg gctcggtgat cgcgaagaac gtctcgtagc cctcgcgcag cgcgggcacc
     1741 gcgtacttct cgatctgcgc cttgctgccg gcccagatcg cgggcggcgg gtagcggccg
     1801 aagacgccgc agcccgggtt gtagagcccc atgcggtgct gcgccatcgc ctcgagaaca
     1861 acgcacatac taaaagtatc gagcccgccg ccgccgtact cctcgcgggc gccgagggtc
     1921 cacaaccccg ccgccttggt cttggcggag agccgtacga agtcctcgcg cgggatctcg
     1981 ggcgcgtcgg gatcgatggt ctgctcgagc ggaatgatct cgtcctggat gaaccgccgg
     2041 atctgatcgc cgagcaggcg cagctcctcc gggagcgtga agccggggaa caccgtcatg
     2101 gtgatgtggc tcctcgagaa ccctgaggct tgtctggccg ctcgccggcc ctgtcaaggc
     2161 gcgagcatag cgccgatggc acaccggggg caatcggcaa cgctccaggc agccgcgcgg
     2221 gctagtgtct tctcgaagag ccggtcagga gccagagaag gttcagaagc gggctatccc
     2281 gctcggaagg ggtcgccgcc tcgagcttcg ccggtgccac ggtcttcttc ggcggcccgg
     2341 cgaccgcttt ctgagggctg cgccccggcg cggcgctcgt gtccccggcc ggccggctca
     2401 ccgaggtcaa cgagccgggt ccctccgccg tggcggatac ggacccgccc ggcggcagga
     2461 ggaggacgcc cgcgacgacc agggccgcca cccgcctccg ccggggcgtc agatgcgtac
     2521 gacgacgacg tcgttgctgg ccaaccaggc cgacaccttg gcccatccgt ccgaatccct
     2581 ttcttcccag gcccggaggg ccgcggccgg gacgaccacc cacgggagac ggttgcgaac
     2641 gacgcgctcc accagcgcgg ccagatcggt ggtcgggccg agctgagtct gcagggccac
     2701 cgcatcacca cgcggtctcg gagactgagt cgccagagtc gatcccatca tcgtctcaac
     2761 cgccgtgctg aaggcaaccg tcgtgccacc cgcgcgatgc caccaggtca ttggaattac
     2821 agtcacgcgc gcgagcgcgg tacgggaatg ccggtaggac cttccggccc gatcgggaac
     2881 ttttcggccg ccctcggaga gtcctgcaga gatttgcagg gagccgcgac ggtcgcacgc
     2941 gccctggcgt tgccaacggc gcgacactac aaggaggatc aaccgaggag gagtgtcgat
     3001 gaacaatcaa aagtggtacg ggcacaagtg gtgcacggtg atcgccacgc tttgcggcgt
     3061 cctcgccgtg gcggggccgg cgggcgccga ttgcgcgtgg gtgctatggt ctcaggtcca
     3121 cgatccaaag cccggcgggt gggtgcttca gaccgcgttc ccgaacgtgg gcgcctgtac
     3181 caaggctctc gaccagcggg agaaagaggg tcgcaaggcg atctacgtca cggagggcgg
     3241 ggccaaggtc aagggcttga ccgaccggcg cgcctcgacc gacctgcttc tcctgtacgg
     3301 cagcgacgcc agcagcggag gggttgcgtg gcaatgcttc cccgagacag tggacccgcg
     3361 aggacccaag gcgaagtgag gcctccgagc gccccgcagc gagcccgatg aatcggggta
     3421 cactcccccg agcggccact tcgaacgagg agaaacgccc gatgcagaag cgctggtccc
     3481 tgtcggtgcc gatggacggc ttcgccctga ccgagctgac cgaggtggcc cgcgaggccg
     3541 agcagctcgg gtacaccgac gcctggtcct tcgaggtgga cggcatcgac ggcttcaccc
     3601 cgctggccgt catcgggctc gccaccaaca tgcggctcgg caccgccatc gccaacgtct
     3661 acacccgcgg gccggccacc ctggcctcca ccgcggccgg gctcgccgac ctggcgcccg
     3721 ggcgcttcgt gctcggcatc ggcgcgggct cgcagcccat cgtggagtcg tggaacggcg
     3781 gccagttcag ccggcccgcc acccgcgtgc gcgagaccgc gcagttcctc cgcaaggcgc
     3841 tggccggcga gcgcgtggtc tggaagggcg agaccttctc ggtggacggc ttccgcctca
     3901 cccgcgtccc cgcccacccg gtgccgatct acgtcgcggc cctccgcgag ggcatgctga
     3961 aggtcgcggg ccaggtcggc gacggcgtca tcctgaactg gctctctccc gacgacgtgc
     4021 cgcgctcggt cggcgtggtg cgcgcggccg cgcggcaggc cgggcgcgat cccgacgcga
     4081 tcgaggtcac cgcgcggctc ttcgtcaacc tcgacactcc cgggcccgag tcggacctgg
     4141 cggtgcgccg ccacatcgcg ggctacctga acgtgccggt ctaccgcgcg ttccaggagt
     4201 ggctcggccg caccgagtcg ctcgggccga tgtggcgtgc gtggggcgcg ggcgatcgcc
     4261 ggggcgcggt ggccgccatc cccgagcagg tgatggacga cctgatgccg cgcgggagcc
     4321 tcgccgacct caaggcccgc atccagctct atctcgaccg cggcatcgac accgcgttcc
     4381 tctcgctcat gaccaccgag acggatccca tcaagaagcg agacctcatg ctgaacgcgc
     4441 tccgcgtcct cgccccg
//
LOCUS       DATMCV010000177         3303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_208634, whole genome shotgun sequence.
ACCESSION   DATMCV010000177 DATMCV010000000
VERSION     DATMCV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208634"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VNU03_04200"
     CDS             <1..847
                     /locus_tag="VNU03_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73685.1"
                     /translation="AIRDASTRAAELRSGGVDIIATPPAPQLEMIDSGDTQVTPVKGG
                     RIIIYPFNVSAAPFNDKKVREAANLAVNKEAIVKNVLGGRGIVMAGPFTPAWLGYDPS
                     VKPFAYDPAKAKALLAEAGFPQGLDTTWSISSGVFLKDTEIAEAVAGQLRAVGIRAKI
                     VPTERAKIQKDAQENSFQGITSVAWGTQFEPDVMLNWVMMRDHMTVPRIKELVLAGRA
                     EVDLEKRRKIYQELYRTAHDEAMWLFVHAQDELWAKRRDIPWTPYSITGSKAIVYYFQ
                     VPGGR"
     gene            837..1532
                     /locus_tag="VNU03_04205"
     CDS             837..1532
                     /locus_tag="VNU03_04205"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWP73686.1"
                     /translation="MAAERRGNGRGTVTAAATRPAHVYDRLKDAIITGMLRPLERISE
                     NKVAADFGLSRTPVRQALQRLEAEGLIQVVPKRGSFVSRPTVEDILEIYQMRTPLEGV
                     CARVAAERIEEDQLATLDRLVKIEQGRGAGRAPDRSLRAAAQFHAVISGCSRNQRMAT
                     LLADLQNQVHRVRVLWPSTVARLGDTWVEHAKIVEALRARDGAEAERLMVEHLERARL
                     STLNRILPSTGTL"
     gene            1558..2073
                     /locus_tag="VNU03_04210"
     CDS             1558..2073
                     /locus_tag="VNU03_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWP73687.1"
                     /translation="MSIAVSTRPVSLVVNGQVRRVEVAPRRTLAELLRENLGLTGTKI
                     GCNRAECGSCTVVLDGRAVFSCSVLAVEAAGRVVQTVEGLAGPDGLHPLQSAFIEHDA
                     VQCGACIPGILMSLKALLDDRLDGSGEIGEQDVREAVGGNLCRCGTYPNTVKATLAAA
                     RALRAGRGARP"
     gene            2070..>3303
                     /locus_tag="VNU03_04215"
     CDS             2070..>3303
                     /locus_tag="VNU03_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP73688.1"
                     /translation="MTPPAGPLEPRPGTASPTAPPKGRLLVHGEVPPSLGRELSVIGR
                     SLDRRDALEKVTGAAMYSSDMTRPGMLHAKILRCPHPHARIRRMDTRRAAALPGVHAV
                     LSCENTAGWHTYWYMIAQPAFPPDIAYAGQEVAVVAAETIDIATQALGLIEVEYDVLP
                     AALTPREAMKPDAPRVPVLDVERPREGNIQQPVYLRQRGDVERGLAEADVVSESCYTL
                     PTQYHVDIQTRCCIAEWDGARLTVYEASQGVWNVKRELAKSLGLEEDQVRVVVQSMGG
                     GFGSKAGAQRVVHYASRLAMLAGRPVRLELTRPEEFMSHPRRYAGEVTMRLGAKRDGT
                     LTALDAEIVLDIGSGSLYAGRYTMTLHQISELYRCPNVRVRIVAVYTNTGPTGPQRGV
                     LDPIATFSTEAAIDDLAAR"
BASE COUNT          500 a         1212 c         1163 g          428 t
ORIGIN      
        1 cgccatccgc gacgcctcca cccgcgccgc cgagctgcgc agcggcggcg tcgacatcat
       61 cgccaccccg cccgcgccgc agctcgagat gatcgacagc ggcgacaccc aggtgacccc
      121 ggtgaagggc gggcgcatca tcatctatcc cttcaacgtg agcgccgcgc ccttcaacga
      181 caagaaggtc cgcgaggccg cgaacctggc cgtcaacaag gaagccatcg tcaagaacgt
      241 gctgggcggc cgcggcatcg tgatggcggg gccgttcacc ccggcgtggc tcggctacga
      301 cccgagcgtg aagcccttcg cctacgatcc cgcgaaggcc aaggcgctgc tggccgaggc
      361 cggcttcccc cagggcctcg acaccacgtg gtccatctcc tccggcgtct tcctgaagga
      421 caccgagatc gcggaggcgg tggcgggcca gctccgggcc gtgggcatcc gcgcgaagat
      481 cgttcccacc gagcgggcca agatccagaa ggacgcgcag gagaacagct tccagggcat
      541 cacctcggtg gcgtggggca cccagttcga gccggacgtg atgctgaact gggtgatgat
      601 gcgcgatcac atgaccgtgc cgcgcatcaa ggagctggtg ctggccggcc gcgcggaggt
      661 cgatctggag aagcgccgca agatctacca ggagctctac cgcaccgcgc acgacgaggc
      721 gatgtggctc ttcgtccacg ctcaggacga gctctgggcc aagcggcgcg acatcccgtg
      781 gacgccctac agcatcaccg ggagcaaggc catcgtgtac tacttccagg tgcccggtgg
      841 ccgctgagcg gcgaggcaac gggcgcggca ccgtcaccgc cgcggcgacg cggccggcgc
      901 acgtctacga ccggctgaag gacgcgatca tcaccggcat gctccgcccg ctcgagcgca
      961 tcagcgagaa caaggtcgcg gcggacttcg ggctgagccg cacgccggtg cggcaggccc
     1021 tgcagcggct cgaggccgag gggctgatcc aggtggtgcc gaagcggggt agcttcgtct
     1081 cgcggcccac cgtcgaggac atcctggaga tctaccagat gcgcactccg ctggagggcg
     1141 tctgcgcgcg agtggccgcg gagcggatcg aggaggacca gctcgccacg ctcgaccggc
     1201 tggtgaagat cgagcagggg cgcggcgcgg gtcgggcgcc ggaccggtcg ctccgcgcgg
     1261 cggcccagtt ccacgccgtc atctccggct gctcgcgcaa ccagcggatg gcgacgctgc
     1321 tcgcggatct ccaaaatcag gtgcaccgcg tgcgcgtgct ctggccctcc acggtggcgc
     1381 gcctcggcga cacctgggtg gagcacgcca agatcgtgga ggcgctgcgg gcccgcgacg
     1441 gcgcggaggc ggaacggctc atggtcgagc acctggagcg ggcgcgcctc agcacgctga
     1501 accgcatcct gcccagcacc gggaccctct gaggctcgcg gtcacgaggt acgcacgatg
     1561 agcatcgccg tctccactcg cccggtgtcc ctggtcgtga acgggcaggt gcgccgggtg
     1621 gaggtcgcgc ctcggcgcac cctggccgag ctgctgcgcg agaacctggg cctcaccggc
     1681 accaagatcg gctgcaaccg cgccgagtgc ggcagctgca ccgtcgtgct cgacggccgc
     1741 gcggtgttct cctgctcggt gctggcggtg gaggccgcgg gacgcgtggt gcagacggtg
     1801 gagggcctgg ccggccccga cggcctccat ccgctccagt ccgcgttcat cgagcacgat
     1861 gccgtccagt gcggcgcctg catcccgggc atcctcatgt cgctgaaggc gctgctggac
     1921 gaccgcctgg atggcagcgg agaaatcggc gagcaggacg tccgcgaggc cgtcggcggc
     1981 aacctctgcc gctgcggcac ctatcccaac accgtgaagg ccacgctggc cgccgcgcgc
     2041 gccctgcgcg ccggccgggg ggcgcgcccg tgaccccgcc ggccggcccg ctcgagcccc
     2101 ggcccggcac cgcgagcccg acggcgccgc cgaagggccg cctgctcgtc cacggcgagg
     2161 tgccgccttc cctcggacgc gagctctccg tcatcggccg ctcgctcgac cgccgcgacg
     2221 cgctcgagaa ggtgaccggc gccgcgatgt actcctccga catgacgcgc ccgggcatgc
     2281 tgcacgccaa gatcctgcgc tgcccgcatc cgcacgcgcg catccgccgc atggacacta
     2341 ggcgcgcggc cgccctgccc ggcgtgcacg cggtgctctc ctgcgagaac accgcgggct
     2401 ggcacaccta ctggtacatg atcgcccagc ccgcgttccc gccggacatc gcctacgcgg
     2461 gtcaggaggt cgcggtggtc gccgcggaga ccatcgacat cgcgacgcag gccctgggcc
     2521 tgatcgaggt cgagtacgac gtcctgcccg ccgcgctcac cccgcgcgag gccatgaagc
     2581 ccgacgcccc gcgcgtgccg gtcctggacg tcgaacggcc ccgtgaaggc aacatccagc
     2641 agccggtcta tctgcgccag cgcggcgacg tggagcgtgg cctcgccgag gcggacgtgg
     2701 tcagcgagag ctgctacacg ctgccgaccc agtaccacgt cgacatccag acccgctgct
     2761 gcatcgcgga gtgggacggc gcgcggctca ccgtctacga ggcctcgcag ggcgtgtgga
     2821 acgtcaagcg cgagctcgcg aagtccctcg ggctcgagga ggaccaggtc cgggtcgtcg
     2881 tgcagagcat gggcggcggc ttcggctcca aggcgggcgc gcagcgggtc gttcactacg
     2941 cctcgcggct ggcgatgctg gccggccgcc cggtgcgcct cgagctgacg cgtccggagg
     3001 agttcatgag ccacccgcgg cgctacgcgg gcgaggtcac gatgcgcctc ggcgcgaagc
     3061 gcgacggcac gctgaccgcg ctcgacgccg agatcgtgct cgacatcggc tcgggcagcc
     3121 tctacgccgg ccgctacacg atgacgctgc accagatctc ggagctctat cggtgcccga
     3181 acgtgcgggt ccggatcgtg gcggtctaca ccaacaccgg gcccaccggg ccccagcgcg
     3241 gggtgctcga ccccatcgcg acgttctcca ccgaggccgc catcgacgac ctggccgcgc
     3301 gcc
//
LOCUS       DATMCV010000178         5007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_742896, whole genome shotgun sequence.
ACCESSION   DATMCV010000178 DATMCV010000000
VERSION     DATMCV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742896"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VNU03_04220"
     CDS             complement(<1..182)
                     /locus_tag="VNU03_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73689.1"
                     /translation="MAERGGDPAADMIPRERAVGGDRGRRPDRHVQIFRRAAGTSRIR
                     LGPGIAVPHTRHISVTA"
     gene            93..482
                     /locus_tag="VNU03_04225"
     CDS             93..482
                     /locus_tag="VNU03_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73690.1"
                     /translation="MAIWAPTSIAPDRALARDHVRGRVASALRHAFPVAWSPEDQAVI
                     AQVRAAYDSYQHASAASKHRLLVTDRFVDLMALAGRPEEVREQVRGIIGVGGVGRIIL
                     LPQVPGQAFVEREEVLRLFAEQVMARL"
     gene            complement(493..1077)
                     /locus_tag="VNU03_04230"
     CDS             complement(493..1077)
                     /locus_tag="VNU03_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP73691.1"
                     /translation="MDAVDLWLLRYEPIHTFVADDLVEGLTEAQVRGRPVPGVNPIAW
                     LVWHAMRIEDVCVNRFVLDRPQVLDAGWTPRLRVSRRDVGTGMTDAQVDALCEAVDVE
                     QLRGYCRAVTRATLDAAPLLRGLDLEVSVPAERVTRVCTEEGAVDPGATWLTEFWAGG
                     RSRGWIVAQTSLLHVYGHYFEGLATKGLWGARSR"
     gene            complement(1085..2002)
                     /locus_tag="VNU03_04235"
     CDS             complement(1085..2002)
                     /locus_tag="VNU03_04235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP73692.1"
                     /translation="MKVGVNLITFGPGASEDSLARWAGFAETVGYHFMMLGDHVTITA
                     DVQGRYPAPFYEPFTTLAWLAGLTRRVELGFTVIVVPYRHPLETARMAATLDRISGGR
                     LIFGVGVGWARQEFEVLGLPFEKRGAMTDDYLGALKAAWTQDVASYQGRFVSFKEIYT
                     APRPLQRPHPPIWVGGSSDAALRRTVRHGDAWHPIRSRIAFLRDEGVPKLRAIADREG
                     RAVPALCPRIRLRLTDQPMPEETRLAGEGTMDQVRRDFEALQKLGVPYLCLDTFADDV
                     EGSRNHEAAWRMLTSLAERAFDLPQQSLR"
     gene            complement(1999..2430)
                     /locus_tag="VNU03_04240"
     CDS             complement(1999..2430)
                     /locus_tag="VNU03_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP73693.1"
                     /translation="MSQFFKILSHVSVTVTDVAKARDFYSGVLGFQEIARPAFDFPGI
                     WYSLGGDLQLHIILNDQLVRDAVEREKIVARYPHFALWTDDADATARRIQERGLVCRD
                     VISGPTGLRQLFVKDPDGNMVEFLGPSASAGQRRMEAVGSH"
     gene            complement(2427..3767)
                     /locus_tag="VNU03_04245"
     CDS             complement(2427..3767)
                     /locus_tag="VNU03_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWP73694.1"
                     /translation="MTRDELKGRVFAAIDRRATEIVALGERIRKHPEMGFKEAKTAGL
                     VQETLGRLGMTPRTGLAMTGVRAEARGRGGDGPTFALIGELDGLRVTGHPEADPATGV
                     AHACGHNAQIAGMLGAAMGLFDAGAFDHLAGRVVFFGVPAEEGGDIEWRQEQIRAGRL
                     EFPCGKQELMRLGLFDDVDLAMMIHTNWRAEDGKAGVPASNNGRVGKTARFTGRAAHA
                     GGAPHMGVNALYAAQIALAGINAVRETFRDEDSIRVHPILTHGGSQVNVIPGEARLEM
                     YVRGKTPEGVADASRRVDRALRAGALALGAQVEIDTLPGPMPLRCDRTMARLFETTAR
                     GLVGPEHYRDIPHRSGSTDMGDLSQVMPVLHPYMGGARGPGHSADFAIVDPDLGYVQP
                     AKALAAMAVDLLADGAAGAREVLAGPRPPMTRDGYLAFQRGIARRERFDGGDPT"
     gene            4066..4371
                     /locus_tag="VNU03_04250"
     CDS             4066..4371
                     /locus_tag="VNU03_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73695.1"
                     /translation="MNDIVGVLIGIGPVVGALLWSGRQDRLRRRADHVRADISAAANR
                     VLGGESVLSVHVEPRVAWRSGRVHISAPGGYEFLIASVSGAVIARLPAHYELVVDPA"
     gene            4380..4865
                     /locus_tag="VNU03_04255"
     CDS             4380..4865
                     /locus_tag="VNU03_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWP73696.1"
                     /translation="MLAKRMLMVITPAVAPALESAARSAAAVARESGGVVRMAWMQPL
                     PPPRVDHHGRVVADTDREMVRLTEIAQDRMRALTWDFGEVEVEQVVRFGRLATELAVE
                     AHAWDADLIGLATPARPTFRHHLRAWHLAWRVTVPLVLLPISRPDGDRRRESIVLPAF
                     R"
BASE COUNT          659 a         1794 c         1830 g          724 t
ORIGIN      
        1 gcggtcaccg agatgtgccg ggtgtgcggc accgcgatgc cggggccgag acggatgcgc
       61 gaggtgccgg ccgcgcgccg gaagatctgg acgtggcgat ctgggcgccg acctcgatcg
      121 cccccgaccg cgcgctcgcg cgggatcatg tccgcggccg ggtcgcctcc gcgctccgcc
      181 acgcgtttcc ggtcgcgtgg agcccggagg accaggcggt gatcgcccag gtccgcgcgg
      241 cctacgactc ctaccagcac gcgagtgccg cgtcgaagca ccggctgctc gtgaccgacc
      301 gcttcgtgga cctgatggcg ctggccggac ggcccgagga ggtacgcgag caggtgcgag
      361 ggatcatcgg cgtgggcggc gtcgggcgga tcatcctgtt accgcaggtg ccggggcagg
      421 ccttcgtgga gcgggaggag gtgctgcgcc tcttcgccga gcaggtgatg gcccggctct
      481 agcgctccgg atctagcggc tgcgcgcgcc ccagaggccc ttggtcgcga gcccttcgaa
      541 gtagtggccg tagacgtgga gcagcgaggt ctgcgcgacg atccacccgc ggctgcggcc
      601 gccggcccag aactcggtga gccaggtggc gccgggatcg accgcgcctt cctcggtgca
      661 cacccgggtg acccgctccg ccggcaccga gacctccagg tcgagcccgc gcaggagcgg
      721 cgccgcgtcc agggtggcgc gcgtcaccgc gcggcagtag ccgcggagct gctcgacgtc
      781 caccgcctcg cagagcgcgt ccacctgcgc gtcggtcatg ccggtgccca cgtcgcgacg
      841 gctcacccgg aggcgcggcg tccagcccgc gtccagcacc tgcggccggt cgaggacgaa
      901 gcggttcacg cacacgtcct cgattcgcat cgcgtgccag accagccacg cgatcgggtt
      961 gacgccgggc accggacggc cccgcacctg cgcctcggtc aggccctcga cgagatcgtc
     1021 ggccacgaag gtgtggatcg gctcgtagcg cagcagccag aggtcgaccg cgtccatgcg
     1081 aggtctagcg caggctctgc tgcggcaggt cgaaggcccg ctcggccagg ctggtcagca
     1141 tgcgccaggc cgcctcgtgg ttgcgcgagc cctcgacgtc gtcggcgaag gtgtcgaggc
     1201 agaggtacgg cacgcccagc ttctggagcg cctcgaagtc gcggcgcacc tgatccatgg
     1261 tcccctcccc ggccagccgc gtctcctccg gcatcggctg atcggtgagg cgcaggcgaa
     1321 tgcgcgggca gagcgcgggg accgcgcggc cctcgcgatc ggctatggcc cggagcttcg
     1381 gcaccccctc gtcgcgcagg aatgcgatgc ggctgcggat cgggtgccag gcgtcgccgt
     1441 ggcgcacggt gcgccgcagg gccgcgtcgc tcgagccgcc cacccagatg ggcggatgcg
     1501 ggcgctgcag cgggcgcggc gcggtgtaga tctccttgaa cgacacgaag cgcccctggt
     1561 agctcgccac gtcctgcgtc cacgccgcct tgagcgcgcc gaggtagtcg tcggtcatgg
     1621 ccccgcgctt ctcgaacggc agcccgagca cctcgaactc ctggcgcgcc cagcccaccc
     1681 ccacgccgaa gatgagccgc cctccgctga tgcggtccag ggtggcggcc atgcgggcgg
     1741 tctcgagtgg gtgtcgatag ggcaccacga tcacggtgaa gcccagctcc acgcggcggg
     1801 tgaggccggc gagccaggcc agcgtggtga acggctcgta gaacggcgcc gggtagcgcc
     1861 cctgcacgtc cgcggtgatg gtgacgtggt cgccgagcat catgaagtgg tagcccacgg
     1921 tctcggcgaa gccggcccag cgcgcgagcg agtcctcgct cgcgccgggc ccgaaggtga
     1981 tgaggttgac gccgaccttc aatgggagcc gaccgcctcc atccgccgct gtcccgcgga
     2041 ggcgctcggg ccgaggaact cgaccatgtt gccgtccgga tccttcacga agagctggcg
     2101 cagcccggtg gggcccgaga tcacgtcgcg gcacacgaga ccccgctcct ggatccggcg
     2161 cgcggtggcg tcggcgtcgt cggtccagag ggcgaagtgc gggtagcgcg ccacgatctt
     2221 ctcgcgctcg accgcgtccc gtaccagctg gtcgttcagg atgatgtgca gctgcaggtc
     2281 gccgcccagg ctgtaccaga tgcccgggaa gtcgaaggcg gggcgcgcga tctcctggaa
     2341 gccgaggacg ccgctgtaga agtcgcgggc cttggcgacg tcggtgacgg tcacgctcac
     2401 gtggctcagg atcttgaaga actggctcat gtcgggtctc ctccgtcgaa gcgctcgcgg
     2461 cgggcgatgc cgcgctggaa cgccaggtag ccgtctcggg tcatgggcgg ccgggggccg
     2521 gcgagcacct cgcgcgcgcc cgccgcgccg tcggcgagca ggtccaccgc catggcggcc
     2581 agggccttgg ccggctgcac gtagcccagg tccgggtcga cgatcgcgaa gtcggcggag
     2641 tggcccggcc cgcgggctcc gcccatgtag ggatgcagca ccggcatcac ctggctcagg
     2701 tcgcccatgt ccgtggagcc ggagcgatgc gggatgtcgc ggtagtgctc ggggccgacc
     2761 aggcctcgcg cggtcgtctc gaagagccgg gccatcgtgc ggtcgcaccg gagcggcatc
     2821 gggcccggca gggtgtcgat ctcgacctgg gccccgagtg cgagcgcgcc ggcgcggagc
     2881 gcgcggtcga cccggcggct cgcgtcggcc actccctccg gagtcttccc gcgcacgtac
     2941 atctccaggc gggcctcgcc ggggatgacg ttcacctgcg agcccccgtg ggtgaggatc
     3001 gggtgcaccc ggatcgagtc ctcgtcgcgg aacgtctcgc gcaccgcatt gatcccggcg
     3061 agcgcgatct gcgccgcgta gagcgcgttc acccccatgt ggggcgcgcc tcccgcgtgg
     3121 gcggcccgcc cggtgaagcg cgcggtcttg cccacccggc cgttgttcga cgcggggacg
     3181 cccgccttgc cgtcctccgc gcgccagttg gtgtggatca tcatcgcgag gtccacgtcg
     3241 tcgaagaggc cgaggcgcat cagctcctgc ttgccacacg ggaactcgag ccggccggcc
     3301 cggatctgct cctgccgcca ctcgatgtcg ccgccctcct cggcgggcac gccgaagaag
     3361 accacgcggc ccgcgaggtg gtcgaacgcg cccgcgtcga agagacccat cgccgcgccg
     3421 agcatgcccg cgatctgcgc gttgtgccca caggcgtgcg cgacgccggt cgcgggatcg
     3481 gcctcggggt gcccggtcac gcgcaggccg tccagctcgc cgatcaacgc gaacgtcggg
     3541 ccgtcgcccc cgcgcccgcg cgcttccgcg cgcaccccgg tcatggcgag gccggtgcgc
     3601 ggggtcatgc ccaggcggcc gagcgtctcc tgcacgaggc cggcggtctt cgcttccttg
     3661 aaccccatct ccgggtgctt gcgaatccgc tcgccgagcg cgacgatctc cgtcgcgcgg
     3721 cggtcgatcg ccgcgaacac ccgccccttc agctcgtctc tcgtcatggc agcagatgct
     3781 agccgattcg cccgggctag gccagggtga gaaaaaatca ggcgcggccg cgccgatctg
     3841 ctcacgcgct gagcggcgca ctcccctcgc ccacgacggc gggcctggcg gtcccacggc
     3901 taactcgcct ccccgtaacg tcgaacgcgc gatgcgcacg cgcgcgatga cgcatggcac
     3961 gatcgctgca gaattcccct tccggagcgc cgggcggggc tccaatcgag cgtcgtcgct
     4021 tgcatcagag cgacggcgtt ttttcttttg aaaaggagac gtcacgtgaa cgacatcgtg
     4081 ggagtgttga tcggcatcgg cccggtggtg ggcgcgctgc tgtggagtgg acggcaggat
     4141 cggcttcggc ggcgggccga tcacgtgcgc gcggacatca gcgcggccgc gaaccgggtg
     4201 ctggggggcg agtcggtcct gtcggtgcac gtggagccgc gggtcgcctg gcgatcgggc
     4261 cgcgtgcaca tctcggcacc cggcggctac gagttcctga tcgcgagcgt gtccggcgcg
     4321 gtcatcgcgc gcctgcccgc ccactacgag cttgtggtgg accccgcatg agcacgcccg
     4381 tgctggcgaa gcggatgctg atggtgatca cgccggccgt ggccccggcc ctggagagcg
     4441 cggcccgcag cgcggccgcg gtggcccgtg agagcggcgg cgtggtgcgg atggcctgga
     4501 tgcagccgct cccaccgccg cgggtcgacc accacggccg ggtcgtcgcg gacaccgacc
     4561 gcgagatggt ccggctgacc gagatcgcgc aggatcgcat gcgggcgctc acctgggact
     4621 tcggcgaggt cgaggtcgag caggtcgtgc ggttcggccg tctcgccacc gagctggccg
     4681 tcgaggccca cgcctgggac gcggatctca tcggcctggc cactcccgca cgaccgacct
     4741 tccgtcacca cctccgcgcc tggcatctgg cgtggagggt gaccgtcccg ctcgtgctgc
     4801 tgccgatttc gcggccggac ggagaccgcc gccgtgagtc catcgtgcta cccgcgtttc
     4861 gctgaccggc caacgcggta acccttgccc ggtagatcct cctcgcgtgg gtcgggccgg
     4921 gcggcggcga acgccctctt ttcggcgatt tttgccgcgc cgcgcgcgtg gcacagcgca
     4981 tgctttctcc cgcgccaggg aggattc
//
LOCUS       DATMCV010000179         2236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_742941, whole genome shotgun sequence.
ACCESSION   DATMCV010000179 DATMCV010000000
VERSION     DATMCV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742941"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VNU03_04260"
     CDS             complement(<1..1200)
                     /locus_tag="VNU03_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWP73697.1"
                     /translation="MKQIVAVLLALGLCPVMASAQTGGDSSRNVGVVTTLAGNATVAR
                     ASLASPQALRFKDDVFVLDRISTAERSIVRVLLGGKALVTVRELSALTITEHTDRSTI
                     DLSSGKIAMGVLRQRMRPGEVIEIRTPNAIAAIRGTVLVVELIPEPGGSPGSPRYTTK
                     VHVLHGLVEVSDPKNPGAPPAQVGALESWSRTGSEPSALVPMSPAAAEQVFVGLHSAP
                     QIAEGPSEFIHNVTVREQAKAVAVAEFLAPEVVGAGAGGDGNGPAALPSAHGATTPGI
                     TDAPVLPLIASNPFQPGAVASGGPTGLARFSFSGQPVTQAGSLYSLSRGLNEALAVPI
                     LEATNSSLTIGQSLMEISGGATFSSTGAAPLLYLDPTQLTAASLLTLRGGGRVSLVGA
                     LFHDQSGA"
     gene            complement(1468..>2236)
                     /locus_tag="VNU03_04265"
     CDS             complement(1468..>2236)
                     /locus_tag="VNU03_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73698.1"
                     /translation="FQGGTLTTSSTEPLAFLRGGTHSFGSMGVAMFDLSGVNTATDAD
                     TGLTVGTDRPLQHAGSLLEASNATVTANQAVRVDTALLEATAPLLALRDGSKLTTARD
                     TVQLSYQAKVTSLGSVMRLDRSALVVAQGAALNLAGGSLLRVTGDLFSLTNGSTLQLL
                     NGPLVSLSGGSLLNVNGALIGFGGTGGNIVSVANNLCPCTTIGGLPVSLTNGALASNV
                     TIAGAIKNGGLGALNVAPNAAVIRVDGAGTKVTINGM"
BASE COUNT          330 a          744 c          825 g          337 t
ORIGIN      
        1 ggcgccgctc tgatcgtgga acagcgcacc cacgaggctg acgcggccgc ctcccctcag
       61 ggtcagcagg ctcgccgcgg tgagctgagt gggatcgagg tagaggagag gtgcggcgcc
      121 cgtgctcgag aaggtggcgc cgcccgagat ctccatgagg ctctgcccga tcgtcagcga
      181 gctgttggtg gcctcgagga tcggtaccgc cagggcctcg ttgaggccgc gcgatagcga
      241 gtagaggctg ccggcctggg taacgggctg gccgctgaag gagaagcgcg cgagcccggt
      301 cggtccgccg gacgcgaccg cgccgggctg gaatgggttg ctcgcgatca gcggaaggac
      361 cggcgcgtcc gtgatgcccg gggtggtggc gccatgggcg ctcggaagtg cggctggccc
      421 gttgccatcg ccgcccgccc cggccccgac tacctcggga gccaggaact cggcgaccgc
      481 caccgccttg gcctgctcgc gaacggtcac gttgtggatg aactcgctcg gtccctccgc
      541 gatctggggc gcggagtgca ggccgacgaa cacctgctcg gccgcggcgg gcgacatcgg
      601 cacgagggcc gacggctcac tgcccgtacg gctccagctc tcgagcgcgc ccacctgcgc
      661 gggcggagcg cccgggttct tggggtcgga gacctcgacc aggccgtgga gcacgtggac
      721 cttggtggtg taccgcgggg acccggggga gccgcccggc tcgggaatca gctccacgac
      781 cagcacggtg ccccggatcg ccgcgatggc gttcggggtc cggatctcga tcacctcgcc
      841 ggggcgcatg cgctgccgca gcacgcccat cgcgatcttg cccgacgaga ggtcgatggt
      901 ggagcggtcg gtgtgctcgg tgatggtgag ggcggagagc tcgcgcacgg tgacgagcgc
      961 cttgcccccg agtagcacgc ggacgatcga tcgctcggcg gtggaaatcc ggtcgagaac
     1021 gaagacgtcg tccttgaagc gcagcgcctg agggctcgcc agggaggctc gcgccaccgt
     1081 cgcgttgccg gccagcgtgg tcacgacccc gacgttgcgg ctcgagtcgc cgccggtctg
     1141 cgccgaggcc atgacggggc acaggcccag ggcgagcagg accgcaacga tttgcttcaa
     1201 gcgtttcatg gcctcgacta ccctccgtgc acaggttgcg cggttgcttt gacgcgacgc
     1261 cgagcatcag agctagcaag cgggttgcca cggggagatc gggcggggtg ccacggaaag
     1321 ggttgaaaaa tgaggcccga tatggcccgg gccgaggcca tcccactaaa acgtgacggg
     1381 ctccggacgc tttttgtccg gagcccgcgt gataccgcga gggtccggcc cccggaggtc
     1441 ggcggccgag cccgctacat tcccccgtta cattccgttg atcgtgacct tcgtgcccgc
     1501 gccgtcgacg cggatgactg cggcattcgg cgccacgttg agcgcgccga gtccgccgtt
     1561 cttgatcgcg cccgctatgg tcacgttgct cgccagggcc ccgttggtca ggctgaccgg
     1621 caggccgccg atcgtcgtgc aggggcagag gttgttggcc accgacacga tgttaccgcc
     1681 ggtgccgcca aagccgatca gcgcgccgtt gacgttcagg agcgagccgc cactcaggct
     1741 gacgaggggg ccgttcagca gctggagagt gctgccgttg gtgaggctga agagatctcc
     1801 tgtcacccgg agcaggctcc cgccggccag attgagcgcc gcaccctgag ccaccacgag
     1861 agcgctgcga tcgaggcgca tcaccgaccc caggctcgtg accttcgcct ggtaggagag
     1921 ctgcaccgta tcgcgcgcgg tcgtgagctt gctgccgtcg cgcagcgcca gcagcggggc
     1981 ggtcgcctcg aggagcgcgg tatccacgcg caccgcctgg ttcgcggtca cggtggcgtt
     2041 cgaggcctcg agcaagctgc ccgcgtgctg cagcggacgg tcggtgccga cagtgagccc
     2101 ggtgtccgcg tcggtcgcgg tgttgacccc ggacaggtcg aacatcgcca cgcccatgct
     2161 gccgaagctg tgcgtgccgc ccctcaggaa cgcgagcggc tcggtggagg aggtcgtgag
     2221 ggtcccgccc tggaac
//
LOCUS       DATMCV010000180         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_875754, whole genome shotgun sequence.
ACCESSION   DATMCV010000180 DATMCV010000000
VERSION     DATMCV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875754"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VNU03_04270"
     CDS             <1..1432
                     /locus_tag="VNU03_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP73699.1"
                     /translation="EWDAVRAARALKAQWSAGTGLPDHSKEFEAMRAAPVVRDQEIAK
                     RGDLAALSAPPAGTRTLAATYRWPMQTHGSIGPSCSVADVRADRVTVWSSSQNVHGFQ
                     GTCARMLGVERDRVRVIYMDGAGSYGANGADDAAAEAALLSKAVGRPVRVQWSRQEEH
                     GLDPKGPAQLLELRGAVDKDGEIAAWETQAWLPISTANLPNIPLVSLDAAGIPQTQGR
                     ATGLIYQNVDPPYAMPNLKAVVHWVADAPLRTSPLRAPGKIANTFAVESFTDEMAALA
                     RVDPLEFRLRRLSNPRGIEVLKRVAARMGWQPRPSPRPLSRGAVLTGRGLAYVHYKHE
                     ETIVAMGMEVAVERATGRIRVTRVVAAQDCGLMINPDCVQAQLEGNILQTLSRALHEE
                     IVYDGDRVTTVDWASYPILTFPEVPKLELDVIQRLDQPPLGAGEAASTPVGAALANAV
                     FDATGVRLRTVPFRPDRVKAALVGRS"
     gene            1429..>2193
                     /locus_tag="VNU03_04275"
     CDS             1429..>2193
                     /locus_tag="VNU03_04275"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP73700.1"
                     /translation="MIEVRRLGPQVGAEILGVDVRNLDDAGFATIYRAWLDHNVAVVP
                     GQELELDDFVTYSRRFGQVVPHPSKMTRHPERPEVTLLGVNKFGADGALDLAIYRRGA
                     EGWHTDGAYDEEPFKATQLYALAVPSRGGDTHFASMYAAYDALPSRLKDRLEGRKGAF
                     TYGGRKKATALLNAEDRDWTPVFHPIIRTHPETGRKGLYFDPGKILYIGGLEAAESDA
                     LIDELTERMIQPEGCYTHRWRRGDIVIWDNRCSYHKA"
BASE COUNT          334 a          780 c          797 g          282 t
ORIGIN      
        1 cgagtgggac gcggtgcgcg cggcgcgcgc actgaaggcg cagtggagcg cggggaccgg
       61 cctgcccgat cattcgaagg agttcgaggc gatgcgggcg gccccggtcg tgcgcgacca
      121 ggagatcgcc aagcggggcg acctggccgc gctgagcgcg ccgcccgcgg gcacgcgcac
      181 gctggccgcg acctatcgct ggcccatgca gacccacggc tccatcggac cgtcctgcag
      241 cgtggccgac gtgcgggcgg accgcgtcac ggtctggagc tcgtcgcaga acgtgcacgg
      301 cttccagggc acgtgcgccc gcatgctcgg ggtggagcgc gaccgggtgc gcgtcatcta
      361 catggatggc gccggctcgt acggcgccaa cggggccgac gacgcggcag cggaggcggc
      421 gctgctctcg aaggcggtcg ggcgcccggt gcgggtgcag tggagccggc aggaggagca
      481 cgggctcgat cccaagggcc ccgcccagct gctggagctg cggggcgccg tcgacaagga
      541 cggcgagatc gcggcgtggg agacgcaggc gtggctgccc atctcgaccg cgaacctgcc
      601 caacatcccg ctggtgtccc tcgacgccgc gggcatcccg cagacgcagg gccgcgccac
      661 cggcctgatc taccagaacg tggacccgcc ctacgcgatg ccgaacctga aagcggtggt
      721 gcactgggtc gcggacgcgc cgctgcgcac ttcgcccctc cgcgcgcccg gcaagatcgc
      781 caacaccttc gcggtggaat cgttcaccga cgagatggcc gcgctggcgc gcgtggaccc
      841 gctcgagttc cggctgcgcc ggctctccaa cccgcgcggc atcgaggtcc tgaagcgcgt
      901 ggcggcgcgc atgggctggc agccgcgccc gtcgcctcgg cctctgagcc gcggcgcggt
      961 gctcaccggg cgcgggctcg cctacgtgca ctacaagcac gaggagacga ttgtggcgat
     1021 gggcatggag gtggcggtgg agcgcgccac cggccgcatc cgggtgaccc gggtggtggc
     1081 ggcccaggac tgcgggctca tgatcaaccc ggactgcgtg caggcccagc tcgagggcaa
     1141 tattctccag acgctctcgc gcgcgctgca cgaggagatc gtctacgacg gcgaccgcgt
     1201 caccacagtg gactgggcct cgtaccccat cctcacgttc ccggaggtac cgaagctcga
     1261 gctcgacgtg atccagcggc tcgaccagcc cccgctgggc gcaggggagg cggcctccac
     1321 cccggtgggc gccgcgctcg ccaacgcggt gttcgacgcg acgggcgtgc gcctgcgcac
     1381 cgtgccgttc cggccggacc gggtgaaggc cgccctcgtg ggccgctcat gatcgaggtg
     1441 cggcggctgg gcccgcaggt cggggccgag atcctgggcg tggacgtgcg aaacctcgac
     1501 gacgcgggct tcgcgacgat ctaccgcgcg tggctcgacc acaacgtggc ggtcgtcccc
     1561 ggccaggagc tcgagctcga cgacttcgtg acctacagcc gccgcttcgg ccaggtggtg
     1621 ccgcacccgt cgaagatgac gcgccacccg gagcggccgg aagtcaccct gctcggcgtc
     1681 aacaagttcg gggccgacgg tgcgctcgac ctggcgatct atcgccgcgg cgccgagggc
     1741 tggcacaccg acggcgccta cgacgaggag cccttcaagg ccactcagct ctatgcgctc
     1801 gcggtgccga gccgcggcgg cgacacccac ttcgccagca tgtacgccgc gtacgacgcg
     1861 cttccatcca ggttgaaaga ccgcctggag ggacgcaagg gcgccttcac ctacgggggc
     1921 cggaagaagg ccaccgcgct gctcaacgcc gaggaccgcg actggactcc ggtcttccac
     1981 ccgatcatcc gcacgcatcc ggagacgggc cggaagggcc tctacttcga cccgggcaag
     2041 atcctctaca taggcgggct cgaggcggcc gagagcgacg cgttgatcga cgagctgacc
     2101 gagcgcatga tccagcccga gggctgctac acgcaccgct ggcgccgggg cgacatcgtg
     2161 atctgggaca atcgctgctc gtaccacaag gcg
//
LOCUS       DATMCV010000181         2787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_743319, whole genome shotgun sequence.
ACCESSION   DATMCV010000181 DATMCV010000000
VERSION     DATMCV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743319"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /locus_tag="VNU03_04280"
     CDS             <1..1983
                     /locus_tag="VNU03_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP73701.1"
                     /translation="YLEPYRDALDRLPRQIADLRQLTLDNPAQTVRVLRLDQLASEQL
                     AILRRGLDLFTLEPDRARALATARQTVLSGQGKQVMDAIRGEVDQMQRVEWDLLRERS
                     AISRASARIALATTVVALAIGLGLVALAVWLFARNLAIRQRAAEVLHAERERFRTTLT
                     SIGDGVVVTDAHGRVTLLNPVAQALTRWDADALGRPIDEVFRIVNEATRSPVENPVAK
                     VLREGAIVGLANHTVLIGRDGTELSIDDSGAPIRDGRGRVAGVVLVFRDITARRAAEQ
                     ELHENDRRKDEFLAMLAHELRNPLAPIRNAAHTLALLGTPDDRIHWVSGVIERQVGLM
                     TRLVDDLLDVSRITSGKITLQREPVSIASVIAQSVEAARPPAESRKETLEVDVDPDVG
                     WVDGDQARLVQAVGNLLDNAIKYTEEGGHIRLTARVDAREVVIAVRDSGVGIAGDLLP
                     HVFDLFTQADRSLARRQGGLGIGLTLVRRLVDMHGGRVEAASEGPGHGSEFTIRLPRL
                     AVDAAEPAAEPAPGAPEGPAAPARRVLVVDDQADSTDSLALFLRLRGHQVHTASDGPG
                     ALEEFARCQPEVVFLRSGAARHERLRRGAAAAGDAGGAGRAAGGGHRLRDRGRPRADP
                     GRRLRRPPRQAGGPARGRGPPHSPRRLRKLLNQGPT"
     gene            complement(1935..>2787)
                     /locus_tag="VNU03_04285"
     CDS             complement(1935..>2787)
                     /locus_tag="VNU03_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73702.1"
                     /translation="QSAAAGAMQGVREGIPGLQDPLRQTLRGSLEDPALVRAARDMTD
                     SAMQTLQARLGSPEMRQEVDLLVTQAMESLHRSGDRTVQALVKGAGDQLEIELRKVAT
                     GSILAATTTLRDSIERDVNPATQRLARQMGEQLVASLVAGLEGPMQARLQLVGRNMSL
                     ALIKGAAEGADDPVNQAGFGGLTHQVMLQAMRGARQGMSEGLPNRTQVALISTVVLLG
                     VLVLGSGGGLAWFWWRYQQSAKTLTIVAESINEHKSDALKDTIRKSAHDNYVGPWFSS
                     FLKRRGL"
BASE COUNT          365 a         1018 c         1024 g          380 t
ORIGIN      
        1 tatctggagc cgtaccgcga cgcgctggac cgcctgccgc gccagatcgc cgacctccgg
       61 cagctcaccc tcgacaaccc ggcccagacc gtgcgcgtgc tgcggctcga tcagctcgcg
      121 agcgagcagc tggccatcct gcgtcgcggt ctcgatctct tcacgctcga gcccgaccgg
      181 gcgcgggcac tcgcgaccgc gcgacagacg gtgctgagcg gccagggcaa gcaggtcatg
      241 gacgcgatcc gcggcgaggt ggaccagatg cagcgggtcg agtgggatct gctccgggag
      301 cgcagcgcga tctcccgcgc gagcgcccgg atcgcgctcg ccaccaccgt cgtcgcgctc
      361 gccatcggcc tgggcctggt ggccctcgcg gtctggctgt tcgcgcgcaa cctggcgatc
      421 cgccagcggg cggccgaggt gctccacgcc gagcgtgagc gcttccgcac cacgctcacc
      481 agcatcgggg acggggtggt ggtgaccgac gcgcacgggc gggtgaccct gctcaacccg
      541 gtcgcgcagg ccctgacgcg ctgggacgcc gacgcgctcg gccggccgat cgacgaggtc
      601 ttccggatcg tcaacgaggc gacccggagc ccggtggaga acccggtggc caaggtcctg
      661 cgggagggcg cgatcgtggg cctcgccaac cacacggtgc tgatcgggcg ggacgggacc
      721 gagctctcga tcgacgacag cggggccccg atccgcgacg gacgcggccg cgtcgcgggc
      781 gtggtcctcg tgttccgcga catcaccgcg cgccgcgcgg ccgagcagga gctgcacgag
      841 aacgaccggc gcaaggacga gttcctcgcc atgctggccc acgagctgcg caatccgctc
      901 gccccgatcc gcaacgcggc ccacaccctg gccctgctcg gcacccccga cgatcggatc
      961 cactgggtgt cgggcgtgat cgagcgccag gtggggctca tgacccgcct ggtggacgac
     1021 ctgctcgacg tctctcgcat cacgtcgggc aagatcacgt tgcagcgcga gccggtctcg
     1081 atcgcgtcgg tgatcgccca gtcggtggag gccgcgcgcc cgcccgccga gagccggaag
     1141 gagacgctgg aggtggacgt ggaccccgac gtggggtggg tggacggcga ccaggcccgc
     1201 ctcgtccagg cggtgggcaa cctgctcgac aacgcgatca agtacacgga ggaaggcggg
     1261 cacatccggc tgaccgcccg cgtcgacgcc cgggaggtcg tgatcgcggt gcgcgacagc
     1321 ggcgtcggca tcgccggcga tctgctgccg cacgtcttcg atctgttcac ccaggcggac
     1381 cgctcgctcg cgcgccggca gggcgggctc gggatcgggc tcacgctggt gcgacggctg
     1441 gtcgacatgc acggcggccg ggtggaggcc gcgagcgagg ggcccgggca cggcagcgag
     1501 ttcaccatcc ggctgccccg gctggccgtc gacgcggccg agcccgccgc ggagccggcg
     1561 cccggcgcgc cggaggggcc ggccgctccc gcgcgccggg tgctggtggt ggacgatcag
     1621 gccgactcca ccgactcgct ggccctgttc ctgcgcctcc ggggccacca ggtccacacc
     1681 gcctccgacg ggccgggcgc cctcgaggag ttcgcgcgct gccagccgga ggtggtcttt
     1741 ctccgatctg gggctgcccg gcatgagcgg ctacgacgtg gcgcggcggc tgcgggcgat
     1801 gccggaggcg cgggacgtgc ggctggtggc ggtcaccggc tacgggaccg aggccgaccg
     1861 cgagcagacc cgggccgccg gcttcgacgt ccacctcgcc aagccggtgg acccgcacgc
     1921 ggtcgaggcc ctcctcacag cccgcgacgc ttgaggaagc tgctgaacca gggccccacg
     1981 tagttgtcgt gcgcgctctt ccggatcgtg tccttgagcg cgtccgactt atgttcgttg
     2041 atgctctccg cgacgatcgt gagggtcttc gcgctctgct ggtagcgcca ccagaaccag
     2101 gcgagcccac ctccgctgcc cagcaccagc acccccagca ggacgaccgt cgagatcagc
     2161 gcgacctggg tccggttggg cagcccctcg ctcatgccct gccgggcccc gcgcatcgcc
     2221 tggagcatga cctggtgggt gaggccgccg aagcccgcct ggttgaccgg gtcgtcggcc
     2281 ccctcggccg cgcccttgat gagggccagc gacatgttgc ggccgacgag ctggagccgc
     2341 gcctgcatcg gcccctcgag cccggccacc agcgacgcga ccagctgctc gcccatctgc
     2401 cgggccagcc gctgggtggc cgggttcacg tcgcgctcga tcgagtcgcg gagggtggtg
     2461 gtggcggcga ggatgctgcc ggtggcgacc ttccgcagct cgatctcgag ctgatccccc
     2521 gcgcccttga ccagcgcctg gaccgtccgg tcgccgctcc ggtggaggct ctccatggcc
     2581 tgggtcacga gcagatcgac ctcctggcgc atctcgggcg agccgaggcg ggcctgcagg
     2641 gtctgcatcg cggagtcggt catgtcgcgc gcggcccgga cgagggcggg atcctcgagg
     2701 ctgccgcgca aggtttgccg caggggatcc tggagaccgg gaatgccttc gcgcacgccc
     2761 tgcatggccc cggcggccgc gctctgt
//
LOCUS       DATMCV010000182         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_144903, whole genome shotgun sequence.
ACCESSION   DATMCV010000182 DATMCV010000000
VERSION     DATMCV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144903"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1525
                     /locus_tag="VNU03_04290"
     CDS             167..1525
                     /locus_tag="VNU03_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWP73703.1"
                     /translation="MTKDSRTPVVDALVDFVLGLELGSMPAAVVAAAGLSFTDWVGGA
                     IRGSTEPLAGALDAVVAATGGEPQATVLGRGRRTSALLAALANGAQGHALDFDDTHLA
                     SIVHGSAPVAPVVLAVAEWHHRPGVAALEAFVAGFEIETRIGRVIGPMLADRGWHVTA
                     VLGHFGAAAAAGRLLGLTAEQLGRALGIAGTQAAGLEQSLGTMCKPLHPGKAAMNGLL
                     AALLAREGFTGPTGILDARPGLPGTFLGVTDLSPALDDLGTRFEILDNSTKLYAACHL
                     LHATIDAGRAIRARAAPSPEAIATVECHVHPLAVKVAAIAEPRTGLEAKFSAAYCAAL
                     ALTGGEAAESDFSATPDPALVELAGRIRPVADLALGIPEARMRVRLRDGRVLDEVVRA
                     ARGTPGHPVSRPDVEEKFRRLAGVVLPAGQVGRLLETLRHLPALADVGALAALTCKGD
                     QP"
     gene            1817..>2043
                     /locus_tag="VNU03_04295"
     CDS             1817..>2043
                     /locus_tag="VNU03_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP73704.1"
                     /translation="MVTAKAVATLDHLAGGGRVLLGIGVGWLAEEFAALGLPFDDRGP
                     RTDEYVAAMRALWSQECATFKGRFISFDQVF"
BASE COUNT          253 a          774 c          730 g          286 t
ORIGIN      
        1 ggacgccggc ctacgatggg gtggcgatca cgtggttcga cgacacccag gccatgcgcg
       61 cgtcggcggc gagcccgcaa tacccgcggg tccgggccga cgagccgaac ttcatcgcgc
      121 ccggcgacgc accgttcatc atcaccaccg agcacgtgat cgtcccgtga cgaaggattc
      181 acgcacgccc gtcgtggatg cgctcgtcga cttcgtcctc gggctcgagc tcgggtcgat
      241 gcccgccgcg gtggtcgccg ccgcgggcct ctccttcacc gactgggtcg gcggggccat
      301 ccgcggctcg accgagccgc tcgccggcgc gctcgacgcg gtggtcgccg ccaccggcgg
      361 cgagccgcag gcgacggtcc tcggccgcgg ccgccggacg agcgcgctgc tggccgcgct
      421 cgccaacggc gcacagggcc acgcgctcga cttcgacgac acgcacctgg cctccatcgt
      481 ccacggctcg gcgccggtgg ccccggttgt gctcgcggtg gccgaatggc atcatcggcc
      541 gggtgtcgcc gccctcgagg cgttcgtggc cggcttcgag atcgagacgc ggatcggccg
      601 cgtcatcggg ccgatgctcg ccgatcgcgg atggcacgtc accgcggtcc tcggccattt
      661 cggcgcggca gcagccgctg ggaggctact gggcctcacc gccgagcagc tcgggcgggc
      721 gctcgggatc gcgggcaccc aggccgcggg gctcgagcag tcgctcggga ccatgtgcaa
      781 gccgctccac cccggcaagg cggcgatgaa cgggctcctg gccgcgctgc tcgcgcgcga
      841 gggcttcacc ggacccaccg gcatcctcga tgcgcgcccc ggcctgcccg gcacgttcct
      901 cggcgtcacc gacctgagcc cggcgctgga cgacctcggc acgcgcttcg agatcctcga
      961 caacagcacg aagctctatg ccgcctgcca cctgctccac gcgacgatcg acgccggccg
     1021 cgcgatccgg gcccgcgcgg cgccttcccc cgaggcgatc gcgaccgtgg agtgccacgt
     1081 gcatccgctc gcggtcaagg tcgcggcgat tgcagagccg cgcaccgggc tcgaggccaa
     1141 gttcagcgcc gcctactgcg cggcgctcgc gctgaccggc ggcgaggccg cggagtcgga
     1201 cttctcggcg acgccggatc cggccctcgt cgagctcgcc ggccgcatcc ggccggtggc
     1261 cgatctcgcg ctcggcattc ccgaggcgcg gatgcgggtc cggctgcgcg acggtcgcgt
     1321 gctcgacgag gtcgtccggg ccgcccgcgg cactcccggc cacccggtgt ctcggccgga
     1381 cgtggaggag aagttccggc ggctcgcggg cgtcgtcctg cccgcggggc aggtcgggcg
     1441 gctcctcgag acgctccggc atctgcccgc gctcgccgac gtcggggcgc tcgcggccct
     1501 cacctgcaaa ggggaccagc catgaagttc ggactgcgat acgcgagcct cgggcgctac
     1561 agcaacggac ccgccgcggt ggagctggcg caggccgccg aggccgccgg cttcgactcg
     1621 atctggacgg tggagcacgt ggtggtgccg cagggctacc agtcgcgcta tccctattcg
     1681 ccttcggggc gcatgggctc gggactcgag gactatccca tcccggaccc gctgatctgg
     1741 ctggcgtaca tcgcctcggc cacccgcacc atcaaagctc ggcaccgcga tcctcatcct
     1801 gccccagcgc aacccggtgg tgaccgcgaa ggcggtggcg acgctcgatc acctggccgg
     1861 cggcggccgg gtgctgctcg gcatcggcgt gggctggctc gccgaggagt tcgccgccct
     1921 cgggctgccg ttcgacgatc gcgggccgcg caccgacgag tacgtggccg cgatgcgcgc
     1981 gctctggagc caggagtgcg cgaccttcaa gggacgcttc atctccttcg accaggtgtt
     2041 ctg
//
LOCUS       DATMCV010000183         3449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_475937, whole genome shotgun sequence.
ACCESSION   DATMCV010000183 DATMCV010000000
VERSION     DATMCV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475937"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VNU03_04300"
     CDS             <1..442
                     /locus_tag="VNU03_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWP73705.1"
                     /translation="PGSSIMPGKVNPTQCEALTMVCAQVMGNDVAVGFGGASGHFELN
                     VYKPLIIHNVLHSVRLLADACASFDTHCARGIEPDRERIRALVERSLMLVTALAPRLG
                     YDRAAGIAKKAHAEGLTLKEAAVKLGYLTAEEFDREVRPESMVG"
     gene            complement(467..3427)
                     /locus_tag="VNU03_04305"
     CDS             complement(467..3427)
                     /locus_tag="VNU03_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP73706.1"
                     /translation="MSWLALRALLRTILIRLAGIAIFTGALGIGLGWLLDVVASEPEW
                     TETAAIAQRAVDRSGLPELLAGQSARSPKRWADESARFVRELPGVVQIKVWDPQGNVV
                     WAVQSAAIGQHSEGQELRDALAGRVAVRFTALAPPTAAAENFVAPNVADLYVPVYTPG
                     AARVVGVVELSQVPLRMDAALERWSQVAWIIALATGGGLCLILLPAAWRSHRRVRAQA
                     SSTAQRSAHRTQELQRTTEQLREALKAVKQRAEETDRMLEVTEAIGSALEQKQLFEVI
                     AHGAARACRVDRGSIFLRDKSGQLMVPVSSQLADQSTAETSGDGAESLRALALEEMPP
                     FLLEAVRRQEPVVISDPGSDPRVPESWVGLFQAKSALVVPLIHQGRTAGVLILDHLKT
                     LHVFTEEEIRLATTLAAQAAVALEKARLYDEIQQRLQQTETLLAVSQAIGSAPDLTEA
                     IRRTTREMVRILGADAGVAWCVTRGGDRFIPLAGYHVPRELREPLSGLPLAPDDPLID
                     LVKRLRTALQSSNSKTDPRFNQPALNLMPHTSLLVQPVQVSDELLGIFAIAWVREAHV
                     FTMDDMRLADGIAKQAAVAIELHRSQRHIIEQERLNAVGKMASGLAHDFNNALVPISG
                     YAEMLLEHQEILQEPAKATKYLQLIMTGVEDAASVVSRLREFYRKREEGETYRPLSLN
                     QMVEQAVALTRPRWRDEALGSGRTILVEADMRPIPKILGSESELREMLTNLIFNAVDA
                     MPDGGNIRIRTGMREAPAAEEPRTTPPVPQVFLEVSDTGTGMTEEVRRRCLEPFFSTK
                     GERGTGLGLPMVFGIVKRHRGTLDIESAVGKGTTFILHLPADAVQTREVQQRTQAPSG
                     PLHVLVVDDEPIARDVLTEYLTGDGHTVQTAVNGRDGFEKFKAGRFSVVITDRAMPEV
                     GGDQLAAMVKEASPATPVVLLTGFGDLMNAAGEKPDGVDLVVKKPIRLGTLREVLVKM
                     TDPQAAAAGAPAAR"
BASE COUNT          457 a         1197 c         1277 g          518 t
ORIGIN      
        1 gccgggcagc tcgatcatgc ccggcaaggt caacccgacc cagtgcgagg cgctcacgat
       61 ggtgtgcgcc caggtgatgg gcaacgacgt cgcggtcggc ttcggcggcg cgtccggcca
      121 cttcgagctg aacgtctaca aaccgctcat catccacaac gtgttgcact cggtgcggct
      181 cctggccgac gcctgcgcct cgttcgatac ccactgcgcg cggggcatcg agcccgatcg
      241 cgagcggatc cgggccctgg tcgagcgctc gctcatgctg gtcaccgcgc tcgctccccg
      301 gctcggctac gatcgggccg ccgggatcgc caagaaagcg cacgcggagg gactcaccct
      361 caaggaagcg gcggtgaagc tcgggtacct gaccgcggag gagttcgacc gggaggtccg
      421 gccggagtcg atggtgggct aggggagtcc ggggccgggc gcccgctcag cgggcagccg
      481 gggcccccgc ggcggcggcc tgcgggtcgg tcatcttgac caggacctcg cggagggtgc
      541 cgagccggat cggcttcttc acgaccaggt ccaccccgtc cggcttctca cccgccgcgt
      601 tcatcaggtc gccgaagccg gtgaggagga cgaccggggt ggcgggggac gcctccttga
      661 ccatcgccgc gagctggtcg ccgccgacct ccggcatcgc ccggtcggtg atgaccaccg
      721 agaagcggcc cgccttgaac ttctcgaagc cgtcgcgtcc gttgaccgcg gtctgcacgg
      781 tgtggccgtc gccggtgagg tactcggtga gcacgtcgcg ggcgatcggc tcgtcgtcga
      841 ccaccagcac gtggagcggg ccgctcggcg cctgggtgcg ctgctgcacc tcgcgggtct
      901 ggaccgcgtc ggcgggcagg tggaggatga acgtggtgcc cttcccgacc gcgctctcga
      961 tgtcgagggt gccccggtgg cgcttgacga tgccgaacac catcggcagg cccagcccgg
     1021 ttccgcgctc gcccttggtg gagaagaacg gctcgaggca gcgccggcgg acctcctcgg
     1081 tcattccggt tccggtgtcg ctcacctcga ggaacacctg gggcaccggc ggggtcgtgc
     1141 gcggctcctc ggcggcgggc gcctcgcgca tgccggtccg gatccggatg ttgccgccgt
     1201 ccggcattgc gtcgaccgcg ttgaagatga ggttggtgag catctcgcgc agctcggact
     1261 cgctgccgag gatcttggga atcgggcgca tgtccgcctc gaccaggatc gtgcggccgc
     1321 tgccgagggc ctcgtcgcgc caccggggcc gggtgagggc caccgcctgc tcgaccatct
     1381 ggttgaggga gagtgggcgg taggtctcgc cctcctcgcg cttgcggtag aactcccgca
     1441 ggcggctcac cacgctcgcc gcgtcctcca ccccggtcat gatcagctgc aggtacttgg
     1501 tggccttggc gggctcctgg aggatctcct ggtgctcgag cagcatctcc gcgtagccgg
     1561 agatcggcac cagcgcgttg ttgaagtcgt gggcgaggcc gctcgccatc ttgccgaccg
     1621 cgttcaggcg ctcctgctcg atgatgtgcc gctggctacg gtgcagctcg atggccaccg
     1681 cggcctgctt ggcgatgccg tcggccagac gcatgtcgtc catcgtgaag acgtgcgcct
     1741 cgcggaccca ggcgatcgcg aagatgccga gcagctcgtc gctgacctgg accggctgga
     1801 ccagcagcga ggtgtggggc atcaggttga gcgccggctg gttgaagcgc gggtcggtct
     1861 tgctgttgct cgactgcagc gcggtgcgga ggcgcttgac caggtcgatg agcggatcgt
     1921 cgggggccag gggcaggccg gagagcggct cgcgcagctc ccgcggcacg tgatagccgg
     1981 cgagcgggat gaagcggtcc ccgccgcgcg tcacgcacca cgcgacgccc gcgtcggcgc
     2041 ccaggatgcg caccatctcg cgggtcgtgc ggcggatggc ctcggtcagg tccggggccg
     2101 agccgatcgc ctggctgacc gcgagcagcg tctcggtctg ctgcaggcgc tgctggatct
     2161 cgtcgtacag gcgcgccttc tcgagggcga ccgcggcctg ggcggccagg gtggtggcga
     2221 gccggatctc ttcctcggtg aagacgtgga gggtcttcag gtggtccagg atgaggacgc
     2281 ccgcggtgcg gccctggtgg atgagcggta ccaccagcgc ggacttggcc tggaagagac
     2341 cgacccagct ctcgggcacg cgcgggtcgc tcccgggatc ggagatgacc accggctcct
     2401 gtcggcgcac cgcctccagg aggaagggcg gcatctcctc gagggccagg gcgcgcaggg
     2461 actcggcgcc gtcgccgctc gtctccgcgg tggactggtc ggcgagctgc gagctgaccg
     2521 gcaccatgag ctggcccgac ttgtcgcgca ggaagatcga gccgcggtcg acgcggcagg
     2581 cacgcgcggc tccgtgggcg atgacctcga agagctgctt ctgctcgagc gcggagccga
     2641 tggcctccgt cacctccagc atccggtcgg tctcctccgc gcgctgcttg accgccttca
     2701 gggcctcccg gagctgctcg gtcgtgcgct gcagctcctg ggtgcggtgg gccgagcgct
     2761 gggccgtcga gctggcctgc gcgcggaccc gtcgatggct ccgccacgcc gcggggagca
     2821 ggatcaggca gaggccgccg ccggtggcga gcgcgatgat ccacgcgacc tggctccacc
     2881 gctcgagcgc cgcgtccatg cgcagcggca cctgcgacag ctccaccacg ccgaccacgc
     2941 gggccgcacc cggagtgtag accggcacgt agagatcggc cacgttgggc gcgacgaagt
     3001 tctcggcggc ggcggtgggc ggcgcgaggg cggtgaagcg caccgccacc cggcccgcga
     3061 gggcgtcccg cagctcctgg ccctcggagt gctgcccgat cgccgcggac tgcaccgccc
     3121 acaccacgtt gccctggggg tcccacacct tgatctgcac gacgcccgga agctcgcgca
     3181 cgaaccgggc gctctcgtcg gcccagcgct tggggctgcg cgcgctttgc ccggccagca
     3241 gctccggcag gccgctgcga tccaccgctc gctgggcgat cgcggcggtc tcggtccact
     3301 cgggctcgga ggccaccacg tcgaggagcc agcccagccc gatgccgagg gcgccggtga
     3361 agatcgcgat cccggccagg cggatgagga tggtgcgcag caacgcccgg agcgctagcc
     3421 aagacatgag ggactcccgt gccggtcgt
//
LOCUS       DATMCV010000184         6270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_276324, whole genome shotgun sequence.
ACCESSION   DATMCV010000184 DATMCV010000000
VERSION     DATMCV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276324"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VNU03_04310"
     CDS             <1..298
                     /locus_tag="VNU03_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73707.1"
                     /translation="VFAANLEASELSEGPAAPDRLASSDIGNVSQVTPTIHAWVQIAA
                     LGTAIHTREFAAAAAAPSARAGLLAGAKLMALSAVDLLADPSRLQAIKEEFTRR"
     gene            295..1602
                     /locus_tag="VNU03_04315"
     CDS             295..1602
                     /locus_tag="VNU03_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWP73708.1"
                     /translation="MRSRLALVGVAVLLTVALVALSLLYMDPFPPRRIVLVTGQPGGT
                     YDAIGHEYKRRLAREGLRVELQSSAGSLENLQRLLRGKADVAFVQGGTYPLVPDPQGR
                     LRGMAALYREPLWIFYRGRPVTDGLASLAGRRTAIGAPGSGTEATARALLTELGLPTS
                     GDTILGLPSMEARRRLEEGSIDAAFFVSSYRDVHVAALLRHPEVRLLGFTRDVAFARN
                     FRYLTPVRLTEGLLDLKADLPREETTLMAPAALLVCRATLHPRVVEQLLSVAREVHGG
                     GSLIDPPGRFPTREGVDLPLHDAADSFLQRGESFTSRLLPYWALRWVVQLRLILLPLV
                     AVWVPLFKILPWVLRWRGNRILERHYALLRDAEAGIDAAAAPEVLRAGIARLEVLREQ
                     IEGLARRLPLQHQRDAYQCRIHVSLVLAEARDRLRELEHAAAP"
     gene            complement(1599..2249)
                     /locus_tag="VNU03_04320"
     CDS             complement(1599..2249)
                     /locus_tag="VNU03_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73709.1"
                     /translation="MYGTNFLVLLGLAFYLVASFVLWQLLLKEPAPSAARDDRPRPRR
                     WPIRLAKAVITLLGGAASGAATLLVVTTVVNAGKAGTLDRSGMLAVWAVGSVVAMLFI
                     GRAPLIRRAVTRTCLSLGVQGVALPLATLVSFVVAGTRLAGSAGTGGERTVAVLGVRL
                     AGHLPAVWIGLGGFCIGLVLVILGDRPRRRVVGRRGASRRFRSSRAGVLSSRGRPR"
     gene            complement(2257..3099)
                     /locus_tag="VNU03_04325"
     CDS             complement(2257..3099)
                     /locus_tag="VNU03_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73710.1"
                     /translation="MSDTSQEHLARRLDRLERENQRLKRLGALALIGLAALVIMGQAA
                     SAPVANTIEAERFILRDASGNARASLGVRPDGTAALALADDTHQERAVFTVTAQGLAA
                     VELSERSGRLRAGLAVRPDGAATLALLDQADHPRVELRSHGDGRPSVTLFDQAGRPRS
                     LLALTAEGATGLTLYDRNGRRRTAIGLEPAGAPAMWLYDGEGNGRAMLAVTSDGQPTL
                     NLSDAGGKGRLWLRVSSDFQLPFLSLHDRDGKQRVSILLRQGGVPQLALGDANERVIW
                     KAAP"
     gene            complement(3111..3395)
                     /locus_tag="VNU03_04330"
     CDS             complement(3111..3395)
                     /locus_tag="VNU03_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73711.1"
                     /translation="MSRATTTMLVWLLAVALLGGCSRSKEDLVEKSRSASTRAELERA
                     LGKPSDIAKLGPVEQWTYKASNGTVVFLIVGDKVTLQAAGGGTDDGKDKK"
     gene            3623..4090
                     /locus_tag="VNU03_04335"
     CDS             3623..4090
                     /locus_tag="VNU03_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWP73712.1"
                     /translation="MITLVVNGHPQRVDAAPDLPLLWVIREDLGLTGTKYGCGMALCG
                     ACTVHLDGTPIRSCVTPISAAAGKKVTTIEGLSPQGTHPVQRAWVELDVPQCGFCQSG
                     QIMSAAALLAQKASPTDADIDSAMAGNICRCGTYQRIRAAIHRAAALKKGGAR"
     gene            4087..6246
                     /locus_tag="VNU03_04340"
     CDS             4087..6246
                     /locus_tag="VNU03_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWP73713.1"
                     /translation="MKAARLSRRALLKGSVAVGAGLTLGFRLFPRGAGSAHAQTAGVF
                     APNQWLTIDRDGLVTITNSVPEMGQGSMTTTAIIIADELDADWNKIRVNQAPADPKRY
                     ANPVTGSQSYGGSRGVRDHMEMWRKSAAAAREMLRQAAADEWGVPVDSVDTEPGVVVH
                     RPTGRKLAYGQLVDRAQALPVPQSPKLKTPDQFRYIGKNLHRLDAPEKATGRGVYGMD
                     VQVPGMLVASIERAPVVSGGKVQSFDATAARAIPGVKHVVQVTHGIAVVADRFSTAVA
                     GRRALKVTWDLGPMAQVSSAQISSEYAALSRQPGQVARKDGDVEKVFAAGGRTLEAAY
                     EVPFLEHACMEPMNATAHVTPDGCVVWAPTQNPGGTQATAARIAGVPPEKVTVNTTLL
                     GGGFGRRGEIDFIVDAVETAKAVGGTPVKLMWTREDDIQHGFYRPATYNVFRAALDGG
                     GIPVAWSHRIVGPGILAQKGRMPGNATVDPAAVTGARDLPYDIPNLQVEWINKDFGVP
                     LGFWRSVGSSQNAFITESFIDELAHSAGKDPFEYRRALLGKSPRHKAVLELAAARASW
                     GSPLPAGRARGIAVAFSYGSYAAHVIEASVAADGAVRVHRVVCAIDCGIAVNPDQVKA
                     QMEGGAVYALTAALYGKITVERGAVQQSNFHDYPMMRINEMPVVETFILDSGAAPGGL
                     GEPGVPPVAPALCNAIFALTGKRVRTLPIRPEELKKA"
BASE COUNT          862 a         2312 c         2276 g          820 t
ORIGIN      
        1 cgtgttcgcg gccaacctgg aggcgagcga gctctccgag gggcccgcgg cgcccgaccg
       61 cctggcctcc tccgacatcg gcaacgtgag ccaggtgacg ccgaccatcc acgcgtgggt
      121 gcagatcgcc gcgctgggca ccgcgatcca cacccgcgag ttcgcggcgg ccgcggcggc
      181 gccgtccgcg cgcgccggcc tcctggccgg ggccaagctc atggcgctga gcgcggtcga
      241 cctgctcgcg gatccgtcgc gcttgcaggc catcaaggag gagttcaccc ggcggtgagg
      301 agccggctcg cgctcgtggg cgtggccgtg ctgctgaccg tggcgctcgt cgcgctgagc
      361 ctgctctaca tggacccgtt cccgccccgc cgcatcgtgc tggtgaccgg gcagcccggc
      421 ggcacctacg acgcgatcgg gcacgagtac aagcgtcggc tcgcgcgcga gggcctccgc
      481 gtcgagctgc agtcgagcgc gggctcgctc gagaacctgc agcgcctgct ccgcggcaag
      541 gccgacgtgg ccttcgtcca gggtgggacc tacccgctcg tccccgatcc gcagggccgc
      601 ctgcgcggca tggccgcgct gtaccgggag ccgctctgga tcttctaccg cgggcggccg
      661 gtgaccgacg gcctcgcctc gctggcgggc cggcgcaccg cgatcggggc gccgggcagc
      721 ggcaccgagg ccaccgcgcg ggcgctgctc accgagctgg gcctgcccac cagcggcgac
      781 accatcctcg gcctgccctc gatggaagcg cgccggcgcc tcgaggaggg cagcatcgac
      841 gcggccttct tcgtctcctc gtaccgcgac gtccacgtgg ccgcgctgct gcgtcacccg
      901 gaggtgcggc tgctcggctt cacgcgcgac gtggccttcg cccggaactt ccgctacctc
      961 accccggtgc gcctcaccga ggggctgctc gacctcaagg cggacctgcc ccgggaagag
     1021 accacgctca tggcgcccgc cgcgctgctg gtgtgccgcg cgacgctgca tccccgggtg
     1081 gtggagcagc tgctctcggt cgcccgcgag gtgcacggcg ggggcagcct catcgacccg
     1141 ccgggccggt ttcccacccg cgagggagtg gacctgccgc tgcacgacgc ggccgactcc
     1201 ttcctgcagc gcggggaatc cttcacgtcg cgcctgctgc cctactgggc cctgcgctgg
     1261 gtcgtgcagc tccgcctgat cctgctcccg ctcgtcgcgg tgtgggtgcc gctcttcaag
     1321 atcctcccct gggtgctccg ctggcgtggc aaccggatcc tcgagcgcca ctacgcgctc
     1381 ctgcgggacg ccgaggccgg catcgacgcg gcggccgcgc cggaggtgct ccgcgccggc
     1441 atcgcgcggc tcgaggtcct gcgcgagcag atcgaggggc tcgcgcggcg cctgccgctc
     1501 cagcaccagc gcgacgccta ccagtgccgc atccacgtct cgctcgtgct ggcggaggcg
     1561 cgcgaccgcc tgcgcgagct ggagcacgcg gcggctccct agcgcggtcg cccgcgggag
     1621 gagagcacgc ccgcgcgcga ggagcggaac cggcgggagg cgccgcgccg ccccaccacg
     1681 cgccggcgcg gccggtcgcc caggatcacg agcacgaggc cgatgcagaa tccgccgagg
     1741 ccgatccaca ccgcgggcag gtgaccggcc agccgaacac cgagcaccgc taccgtgcgc
     1801 tcgcccccgg tgccggcgga gccggccagc cgggtgcccg ccaccacgaa cgagacgagg
     1861 gtggccagcg gcagcgccac gccctgcacg cccagggaga ggcaggtgcg cgtcaccgcg
     1921 cgccggatca gcggcgcccg tccgatgaac agcattgcca ccaccgaccc gaccgcccac
     1981 accgcgagca tgcccgaccg gtccagggtg cccgccttgc ccgcgttcac caccgtggtg
     2041 accaccagca gcgtggccgc gccggaggcg gcgccgccca gcagggtgat caccgccttg
     2101 gcgaggcgga tcggccagcg gcgggggcga ggccggtcgt cgcgcgcggc ggagggcgcg
     2161 ggctccttga gcagcagctg ccagaggacg aagctcgcca ccagatagaa ggcgagcccg
     2221 agcagcacca ggaaattggt gccgtacatg cggcttctac ggcgccgcct tccagatcac
     2281 ccgctcgttg gcgtctccga gggccagctg cggcactccg ccctggcgca ggaggatcga
     2341 cacgcgctgc ttgccgtccc gatcgtgcag actcaggaac gggagctgga agtccgagct
     2401 gacccgtagc cagagacgcc ccttgccccc ggcgtcggac aggttgagcg tgggctggcc
     2461 gtccgaggta accgcgagca tcgcgcgccc gttgccctcg ccgtcgtaga gccacatcgc
     2521 gggggcgccc gccggctcga gtccgatcgc ggtgcggcga cggccgttgc gatcgtagag
     2581 cgtgaggcca gtggcgccct cggcggtcag ggccagcagc gagcgggggc ggccggcctg
     2641 gtcgaagagg gtgacgctcg ggcggccatc cccgtgcgag cggagctcga cgcggggatg
     2701 gtccgcctga tcgagcagcg ccagcgtcgc ggccccgtcc ggccgcaccg cgagcccggc
     2761 ccggagccgc ccgctccgct cggagagctc gaccgcggcg agcccctggg cggtgacggt
     2821 gaagaccgcg cgctcctggt gagtgtcgtc ggccagcgcg agggccgcgg tgccgtccgg
     2881 ccggaccccg aggcttgcgc gcgcgttgcc ggaggcatcg cgcaggatga agcgctcggc
     2941 ctcgatggtg ttggccaccg gggccgaggc ggcctggccc atgatgacga gggcggcgag
     3001 gccgatcagg gccagcgcgc ccagccgctt caggcgctgg ttctcgcgct cgaggcggtc
     3061 gagccgccgg gcgaggtgct cttgactcgt gtcgctcacg gtcgatcctc ctacttcttg
     3121 tctttgccgt cgtccgtccc gccaccggcc gcctgcagcg tgaccttgtc acccacgatg
     3181 aggaagacga cggttccgtt cgaggccttg taggtccact gctccaccgg gcccagcttg
     3241 gcgatgtcgc tcggcttgcc gagcgcccgc tccagctctg cgcgcgtgga cgcgctccgt
     3301 gacttctcga ccaggtcttc cttgctccgg ctgcagccgc ccagcagggc caccgccagc
     3361 agccacacca gcatcgtcgt cgtcgctcga gacatggcgc gctcctcccg gggccggcat
     3421 gacgcggatt gaacccgccg agtctatcag agtgcccact tctcacgcac cgggggaggt
     3481 gacgcgggcc gcaggcccgg cgcggcacgg cgagggcgcc ggtgctacac tgccggcgga
     3541 tcgtccatcg ggagccggcg cggcccgagc tcccgcccgc agttgaggcg acctgctccc
     3601 tgggagcacg gaggagacct cgatgatcac tctcgtcgtc aacggtcacc cacagcgcgt
     3661 ggatgcggcc ccggacctgc ccctgctctg ggtgatccgc gaggacctgg gcctcaccgg
     3721 aaccaagtac ggctgcggca tggccctctg cggcgcctgc acggtccacc tcgacggcac
     3781 gccgatccgc tcctgcgtga caccgatctc ggccgcggcc ggaaagaagg tcaccacgat
     3841 cgaggggctc tcgccccagg ggacgcaccc cgtgcagcga gcgtgggtgg agctggacgt
     3901 gccgcagtgc gggttctgcc agtcggggca gatcatgtcc gccgccgcgc tgctggccca
     3961 gaaggcctcg cccaccgacg ccgacatcga ctcggcgatg gccggcaaca tctgccggtg
     4021 cggcacctac cagcggatcc gcgccgccat ccatcgggcc gccgcgctca agaagggagg
     4081 cgcgcgatga aggccgcgag gctcagccgc cgcgcgctgc tcaaggggag cgtcgcggtc
     4141 ggggccggcc tgaccctcgg cttccggctc ttcccgcgcg gggccggctc ggcccacgcg
     4201 cagaccgcgg gcgtcttcgc gcccaatcag tggctcacca tcgaccgcga cgggctcgtg
     4261 accatcacga actcggtgcc cgagatgggc cagggctcga tgaccacgac cgccatcatc
     4321 atcgcggacg agctcgacgc cgactggaac aagatccggg tcaaccaggc gcccgccgat
     4381 cccaagcgtt acgccaaccc ggtcaccggc tcccagtcct acggcggcag ccgcggcgtg
     4441 cgcgaccaca tggagatgtg gcggaagtcg gccgccgccg cccgcgagat gctcaggcag
     4501 gccgccgccg acgagtgggg cgtgccggtc gactcggtgg acaccgagcc gggcgtggtg
     4561 gtgcatcggc cgaccggccg caagctcgcc tacggccagc tcgtggaccg ggcccaggcc
     4621 ctcccggtgc cgcagagccc caagctcaag accccggacc agttccgcta catcggcaag
     4681 aacctccatc ggctcgacgc gcccgagaag gcgaccgggc gcggcgtcta cggcatggac
     4741 gtgcaggtgc ccggcatgct ggtggcctcg atcgagcggg ccccggtcgt ctccgggggc
     4801 aaggtgcaga gcttcgacgc caccgcggcc agggccatcc ccggcgtcaa gcacgtggtc
     4861 caggtgaccc acggcatcgc agtggtagcc gaccggttct cgaccgcggt ggcggggcgc
     4921 cgcgcgctca aggtgacgtg ggacctgggc ccgatggcgc aggtgagcag cgcgcagatc
     4981 tcgagcgagt acgcggcgct ctcccggcag ccgggccagg tcgcgcgcaa ggacggggac
     5041 gtggagaagg tgttcgcggc ggggggccgg acgctcgagg cggcctacga ggtgcccttc
     5101 ctggagcacg cgtgcatgga gcccatgaac gcgaccgcgc acgtcacccc ggacggctgc
     5161 gtggtgtggg cgccgaccca gaatccgggc ggcacccagg ccaccgcggc gcgcatcgcg
     5221 ggggttcccc ccgagaaggt caccgtgaac accacgctgc tgggcggcgg cttcgggcgg
     5281 cgcggcgaga tcgacttcat cgtcgacgcg gtggagaccg cgaaggcggt gggcggaacg
     5341 ccggtcaagc tcatgtggac gcgggaggac gacatccagc acgggttcta ccgccccgcc
     5401 acctacaacg tcttccgcgc ggcgctggac gggggcggca tcccggtcgc gtggtcgcac
     5461 cgcatcgtcg gcccgggcat cctggcccag aagggccgca tgcccggcaa cgccaccgtg
     5521 gacccggctg cggtcacggg agcgcgcgac cttccgtacg acatcccgaa ccttcaggtg
     5581 gagtggatca acaaggactt cggcgtgccg ctcggtttct ggcgctcggt cggctcctcg
     5641 cagaacgcgt tcatcaccga gagcttcatc gacgagctgg cccactcggc cggcaaggat
     5701 cccttcgagt accggcgagc gctcctcggc aagtcacccc gtcacaaggc ggtgctcgag
     5761 ctggccgcgg cgcgcgccag ctggggctcg ccgctgcccg cgggtcgcgc gcgcggcatc
     5821 gcggtggcct tctcctacgg cagctacgcc gcgcacgtga tcgaggcgtc ggtggccgcg
     5881 gacggagcgg tgcgcgtgca tcgcgtggtg tgcgcgatcg actgcggcat cgcggtcaac
     5941 ccggatcagg tcaaggccca gatggagggg ggcgcggtct atgcgctcac cgccgcgctc
     6001 tacgggaaga tcaccgtcga gcgtggcgcg gtgcagcagt cgaacttcca cgactacccg
     6061 atgatgcgga tcaacgagat gccggtcgtg gagacgttca tcctggactc gggggcggct
     6121 ccgggtgggc tgggggagcc gggggtgcca ccggtggcgc cggcgctctg caatgcgatc
     6181 ttcgcgctca ccggcaagcg ggtccggacg ctgccgatcc ggcccgagga gctgaagaag
     6241 gcctgagccg ccgcgaggta gtgcgcggcc
//
LOCUS       DATMCV010000185         4643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_411154, whole genome shotgun sequence.
ACCESSION   DATMCV010000185 DATMCV010000000
VERSION     DATMCV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411154"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..457)
                     /locus_tag="VNU03_04345"
     CDS             complement(68..457)
                     /locus_tag="VNU03_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWP73714.1"
                     /translation="MRKSGSRMIDEQIKELGDWRGTMLSRLRRLIKQADPEVVEEWKW
                     RGVPVWSHDGLICTGETYKNVVKMTFARGAALEDPSGLFNASLDGNTRRAIDFHEGEK
                     IDERALKALIRAAVTLNASRARGLSRR"
     gene            736..2808
                     /gene="ppk1"
                     /locus_tag="VNU03_04350"
     CDS             736..2808
                     /gene="ppk1"
                     /locus_tag="VNU03_04350"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HWP73715.1"
                     /translation="MSPVPPDAFVNRELSWLEFNRRVLEEAQDPTVPLLERVKFLAIF
                     SSNLDEFFMVRVAELKRRIRAGDTTMGPDEMSPTKTLAAVADQVHRLVDAQHRCFLEE
                     LGPILASEGVRLVRPKEVTAEQERFLEDYFRRTLMPVLTPLAIDPGHPFPHLANRSLC
                     LIVSVKPVAPSVLPHATLTVVRIPGQVVPRFVALPGPPGQHAFMLLEDVIRLQLPTLY
                     TGYEIVSCHAIRVTRDAEIALPRKTAADLLTSVEAGVRERRMGDAVRLQYDADLPAPL
                     LAMLVDELELGPDDLYAGTGFTAFSDLFQLYAAVDLPRLKDHVLPPRPVPAFEGAADI
                     WSAIRAGDVLAHHPYHSFDAVTRFVSDAAEDPRVLAIKMTLYRVSPTSPIAQALTRAA
                     ENGKEVAVMVELQARFDEEANIRWARALEEVGAHVVYGLPGYKTHCKACLVVRAETDG
                     IRRYCHLATGNYNVRTGGIYGDFSLFTCRESIGEDVTELFNMLTGYTRPRRFRHLLMA
                     PTGMREAFQQRIRREAEHARRSEPARIVAKMNSLVDRTLIEELYAASQAGVRIDLIVR
                     GICCLRPGIPGFSDRIRVVSIVDRYLEHARAYYFHNGGHSEYWLASADWMPRNLDHRV
                     EIAFPILDPRLQSQIREVLEIQLTDTSKAREIQIDGSSIRVRAPEAPPLRSQERLYGL
                     VSAESRTS"
     gene            2999..3199
                     /locus_tag="VNU03_04355"
     CDS             2999..3199
                     /locus_tag="VNU03_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73716.1"
                     /translation="MTSTDLLTDLGRSSDLARLVERVARDQLPWVVVSGTAVTAWMRR
                     DPDGWAKVSAWLAERGVAVVRI"
     gene            complement(3207..4586)
                     /gene="sthA"
                     /locus_tag="VNU03_04360"
     CDS             complement(3207..4586)
                     /gene="sthA"
                     /locus_tag="VNU03_04360"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HWP73717.1"
                     /translation="MTDYDLIVIGSGPAGHHAAIQGAKLGRRVAIVERQPGLGGVCMN
                     TGTIPSKTLREAVLYLSGVHQRGFYGVSYRVKPQITMQDLMFRCHQVIQHEVDVYRAQ
                     FGRNGVDVLQGQAVFQDPHTIRVTGGAGEGLFRAERFVIATGTTPVLPRGVPTDHAEV
                     IDSDGLFRLTNIPRSMIVVGGGVIGVEYACMFSTLGVAVTLVDGRRRILDFLDTEIVE
                     ALVYHMRTNGVTFRLGEVVDRVECVEGRHVVAHLASQKQLQAETLLCAAGRQGNTDTL
                     NLAAAGLEADARGRLAVDDGFRTAVPHIYAAGDVIGFPSLASVSMEQGRVASANAFGI
                     PTVTTPALFPYGIYTIPEVSFVGLTEEQLTEARVPYEVGIANYREIARGGIIGDTTGR
                     LKLVFHRETGAVLGVHIIGDGATELVHIGQAVISLGGTIRYFVDHVFNYPTLAECYKV
                     AALAGINKL"
BASE COUNT          681 a         1695 c         1531 g          736 t
ORIGIN      
        1 cgtcatctgc acccgcgggg tccgggacgg cgtcctgctg cacgaggcct cgaggagcag
       61 agccgcccta cctccgagat aggccgcggg cgcgagacgc gttcagcgtc acggccgcgc
      121 gaatgagcgc cttcaatgcc cgctcgtcga tcttctcgcc ctcgtggaag tcgatggcac
      181 gccgggtgtt gccatcgagg ctcgcattga agaggcctga cgggtcctcg agggcggccc
      241 ccctggcaaa ggtcatcttc acgacgttct tgtacgtctc gccggtgcag atcaatccgt
      301 cgtgagacca caccgggacc cccctccact tccactcctc gaccacctcg gggtcggctt
      361 gcttgatcaa tctgcggagc cgggagagca tcgtgccccg ccagtcgccc agctccttga
      421 tctgctcgtc tatcatccga gacccgctct tcctcatgac attgcgcctc acgtgctgat
      481 tctagctgga gccgggatcg accatagttc ccagttactt ccacctcctc atctgcaccc
      541 gccgactccg gtacagcggc ctgctgcacg aggccgcgcc gaggagcgga gccaccctat
      601 ctgtgagata gtattgacgt tgtcgaacgt aacaattccg taatttgacg ccctggcgct
      661 ggccgaggac actgctgccc atgttcgtcc cgccctcgtc gtctttcctg tccggctagc
      721 gccagcgagg tcgtcatgag cccggttccc cccgacgcgt tcgtcaatcg cgagctgtcc
      781 tggctcgagt tcaaccgccg tgtgctcgag gaggcccagg acccgaccgt cccgctgctc
      841 gagcgggtca agttcctggc catcttcagc tcgaacctcg acgagttctt catggtgcgg
      901 gtcgcggagc tgaagcgccg gatccgcgcg ggcgacacca ccatggggcc ggacgagatg
      961 tccccgacga agaccctcgc cgcggtcgcc gaccaggtcc accggctggt cgacgcccag
     1021 caccgctgct tcctcgagga gctggggccc atcctcgcgt cggaaggcgt ccggctcgtc
     1081 cgtcccaagg aggtcaccgc cgagcaggag cggttcctgg aggactattt ccggcggaca
     1141 ctcatgccgg tcctcacccc gctggcgatc gaccccgggc atccgttccc gcacctcgcc
     1201 aaccgctcgc tctgcctgat cgtctcggtc aagccggtgg cgccgagtgt cctgccgcac
     1261 gccaccctga ccgtggtgcg catcccgggt caggtcgtgc cccgcttcgt ggcgctgccc
     1321 gggccgccgg gccagcacgc gttcatgctg ctcgaggacg tgatccgcct ccagctaccc
     1381 acgctctaca ccggctacga gatcgtctcc tgccacgcca tccgggtcac ccgcgacgcc
     1441 gagatcgcgc tcccccgcaa gaccgcggcc gacctcctga cgagcgtgga ggcgggagtg
     1501 cgggagcgcc gcatggggga cgcggtccgg ctgcagtacg acgccgacct gcccgccccg
     1561 ctcctcgcga tgctggtcga cgagctcgag ctggggccgg acgacctcta cgcgggcacc
     1621 ggcttcaccg ccttctcgga tctcttccag ctctacgccg cggtggatct cccgcggctc
     1681 aaggaccacg tcctgccgcc gcggccggtg cccgccttcg agggcgcggc cgacatctgg
     1741 agcgcgattc gcgccggcga cgtgctcgcg catcacccgt accactcctt cgacgcggtc
     1801 acccgcttcg tgagcgacgc cgcggaggac ccgcgcgtcc tcgcgatcaa gatgaccctc
     1861 taccgggtga gccccacctc gccgatcgcg caggccctga cccgggccgc cgagaacggc
     1921 aaggaggtgg cggtgatggt cgagctgcag gcgcgcttcg acgaggaggc caacatccgc
     1981 tgggcgcgcg cgctggagga ggtcggggct cacgtggtct acggcctgcc cggctacaag
     2041 acccactgca aggcctgcct cgtggtgcgc gcggagacgg acgggatccg ccgctactgc
     2101 cacctcgcga ccggcaacta caacgtccgg accggcggca tctacgggga cttcagcctc
     2161 ttcacctgcc gggagtccat cggcgaggac gtgactgagc tgttcaacat gctcaccggc
     2221 tacacgcggc cccgccggtt ccgccacctc ctgatggccc cgaccggcat gcgggaggcc
     2281 ttccagcagc gcatccgccg cgaggccgag cacgcccgcc ggagcgagcc ggcccgcatc
     2341 gtggccaaga tgaacagcct cgtggaccgc accctcatcg aggagctgta cgcggcgagc
     2401 caggccgggg tccgcatcga cctgatcgtg cgcgggatct gctgcctccg gccgggcatc
     2461 cccggcttct ccgatcgcat ccgggtggtc tcgatcgtgg accgctacct cgagcacgcc
     2521 cgcgcctact acttccacaa cggcggccac tccgagtact ggctggcctc ggcggactgg
     2581 atgccgcgga acctcgacca ccgggtcgag atcgcgttcc cgatcctcga cccgcgcctg
     2641 cagtcccaga tccgcgaggt gctcgagatc cagctcaccg acacctccaa ggcgcgcgag
     2701 atccagatcg acggcagctc gatccgagtt cgcgcccccg aggcgccccc gctccgctcg
     2761 caggagcggc tctacgggct ggtctccgcg gagagccgga cctcctagcc cgcctggcgc
     2821 tcaggcgagc gccatcaagt cctaccgcgc ctggccgtag gccggcgacc agcgtcacga
     2881 gcgagggttc gggagcaggt cgcgcgggag cggctgggcc gccgggagcg tcacgaacac
     2941 gtccgcgcgg cgtgacgtcg gtgtcaccct ctcgcgctac cgtttgaggg acaccgccgt
     3001 gaccagcacc gacctcctca ccgatctcgg acggagctcc gatctcgccc ggctcgtgga
     3061 gcgcgtggct cgcgatcagc tcccgtgggt ggtcgtgtcg ggcacggcgg tgacggcgtg
     3121 gatgcggcgg gatccggacg ggtgggcgaa ggtctcggcg tggctcgccg agcggggagt
     3181 cgcggtcgtc cggatctgac ggctggctag agcttgttga tcccggcgag cgccgcgacc
     3241 ttgtagcact cggccagggt cggatagttg aacacgtggt cgacgaagta ccggatcgtc
     3301 cctccgagcg agatgaccgc ctgcccgatg tggacgagct cggtcgcccc gtccccgatg
     3361 atgtgaacgc ccagcaccgc gccggtctcc cggtggaaga cgagcttgag ccggccggtg
     3421 gtgtcgccga tgatcccgcc gcgggcgatc tcgcggtagt tggcgatgcc gacctcgtag
     3481 ggtacccggg cctccgtgag ctgctcctcg gtgagcccga cgaacgacac ctcggggatc
     3541 gtgtagatcc cgtacgggaa cagggcgggc gtcgtcacgg tcgggatccc gaacgcgttg
     3601 gcgctggcca cccgcccctg ctccatcgag accgaggcca ggctcgggaa gccgatcacg
     3661 tcgccggccg cgtagatgtg gggcaccgcg gtgcggaacc cgtcatccac cgcgaggcgg
     3721 ccgcgggcgt cggcctcgag tcccgccgcc gcgaggttca gggtgtcggt gtttccctga
     3781 cggcccgccg cgcagagcag ggtctcggcc tggagctgct tctggctggc caggtgcgcg
     3841 acgacgtgcc ggccctcgac gcactccacc cggtccacca cttcccccag gcggaaggtc
     3901 actccgttgg tgcgcatgtg gtacacgagc gcctcgacga tctcggtgtc gaggaagtcg
     3961 aggatccgcc gccgcccgtc caccagggtc accgcgaccc cgagggtcga gaacatgcac
     4021 gcgtactcga ctccgatcac tccgccgccc accacgatca tgctgcgggg gatattggtc
     4081 agccggaaga gcccgtccga gtcgatgacc tcggcgtggt cggtcgggac gccgcgcggc
     4141 agcacgggcg tcgtgccggt ggcgatcacg aagcgctcgg cccggaagag tccctcgccg
     4201 gcgccgcccg tcacccggat cgtgtgggga tcctggaaga cggcctggcc ctggagcacg
     4261 tccactccgt tgcggccgaa ctgggcgcgg tagacgtcga cctcatgctg gatgacctgg
     4321 tggcaccgga acatgaggtc ctgcatcgtg atctgcggct tcacccggta ggagaccccg
     4381 tagaacccgc gctggtgcac ccccgagaga tagagcaccg cctcgcgcag cgtcttcgag
     4441 gggatcgtcc cggtgttcat gcacaccccg ccgagccccg gctgccgctc cacgatggcg
     4501 acccggcgcc cgagcttcgc gccctggatg gccgcgtggt gcccggccgg tccgctgccg
     4561 atgacgatca ggtcgtagtc ggtcatgacc cgcccatgaa agcacggacg cgtggcgctc
     4621 tcaacacccg ggtatcggag ccg
//
LOCUS       DATMCV010000186         2917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_943526, whole genome shotgun sequence.
ACCESSION   DATMCV010000186 DATMCV010000000
VERSION     DATMCV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943526"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /gene="ybgF"
                     /locus_tag="VNU03_04365"
     CDS             <1..452
                     /gene="ybgF"
                     /locus_tag="VNU03_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HWP73718.1"
                     /translation="PPPAPVAPRPSAPTVTKEVTPEKLFATAMTSFRAEEHGQAVLEF
                     TELIEKYPKHALAATSQYWIGEAYYRQRDFNQALTEFQKVPDLYPQSQSVAESLLKIG
                     MCYRALHDVPRARETWEQVVRSHPKSEAATQARALLASLGAPGRTPR"
     gene            579..1535
                     /gene="dprA"
                     /locus_tag="VNU03_04370"
     CDS             579..1535
                     /gene="dprA"
                     /locus_tag="VNU03_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWP73719.1"
                     /translation="MTHERIHDDTVRDHAGAARALTRGDAEYPALLLAVPTAPSTLHV
                     RGSLVEGDALAVAVVGSRRATPYGLEVAETLAADLAARGVTIVSGLARGIDTAAHRGA
                     LRVGGRSIAVLGSGVDVVYPPENARLAEEIVARGALVSQFAPGTPPLPHHFPTRNAVI
                     AALSLAVVVVEAAERSGSLITARLAAELGREVLATPGRVTASESRGANRLIQDGAALA
                     LGWEDVVAALPERWKACVDTRERIEATGARGAAAASGAGRPSAATRRVLALLGEDPLE
                     IDHVIERSGLGAGPVSAALLDLELEGRVRQIEGKRFVRVGRG"
     gene            1548..>2917
                     /gene="topA"
                     /locus_tag="VNU03_04375"
     CDS             1548..>2917
                     /gene="topA"
                     /locus_tag="VNU03_04375"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWP73720.1"
                     /translation="MAKRSLVVVESPTKVKTIQKYLDSKYIVKASMGHVRDLPKSKLG
                     VDEKKGFKPEYKVLPAKKKVLDELKKAAEKVDALYIATDPDREGEAIGWHLAQELGVS
                     KSKTHRIMFNEITERAVKAAFLHPGKIDTNKVNAQQARRVLDRLVGYKLSPLLWEKIR
                     RGLSAGRVQSVAVRLITEREREIAAFVPVEYWSLHARLKGKNPPDFTATLKEVKDQKA
                     DLPDEASTMTVMTALHGQAWTVKSVTRGERRRNPAPPFITSTLQQDASRKLHFSAKKT
                     MMLAQQLYEGVELGEEGAVGLITYMRTDAVRVAAEAQEEARQWVAGRLGQEYLPDAPP
                     TYKAKKSAQEAHEAVRPSSVAREPKAVARFLSKDQLALYRIIWERFLASQMLPAVYDT
                     VAADIVAGDCLFRAQGSTLKFAGFTAVYIESREATDVEPEEEAEAVVPPLTVGEILKL
                     LALDPK"
BASE COUNT          461 a         1003 c         1085 g          368 t
ORIGIN      
        1 cgccgccgcc cgcgccggtc gcgccccggc cgtccgcgcc gacggtcacg aaggaggtca
       61 ccccggagaa gctcttcgcc accgcgatga cgagcttccg cgccgaggag cacgggcagg
      121 cggtgctcga gttcaccgag ctcatcgaga agtacccgaa gcacgcgctc gccgcgacct
      181 cccagtactg gatcggggag gcctactacc ggcagcgcga cttcaaccag gccctgaccg
      241 agttccagaa ggtgcccgat ctctatccgc agagccagtc ggtggcggag tcgctgctga
      301 agatcggcat gtgctatcgc gcgctgcacg acgtgccgcg cgcgcgcgag acgtgggagc
      361 aggtggtgcg atcgcacccg aagtccgagg ccgccacgca ggcgcgcgcg ctgctcgctt
      421 cactcggagc gcccggtcgc acgccgcggt gagcgggcgc gcccccggcg ggtgcgcggg
      481 ccctcgcggc cgcggtttgt tgacccaccc cggtcgtccg gtatactgaa acgcactgcc
      541 ctgatctgtc atctgaagac gtgaggcgaa cgatacacgt gacccacgag cgtatccacg
      601 acgacacggt gcgagatcac gcgggcgcgg cgcgcgcgct cacccgcggt gacgcggagt
      661 atcccgcgct cctgctcgcg gtccccaccg cgccctccac gctgcacgtg cggggctcgc
      721 tcgtcgaggg agacgcgctc gcggtcgcgg tggtgggatc gcgccgcgcg acgccgtacg
      781 ggctcgaggt ggccgagacg ctcgcggcgg acctggccgc gcgcggcgtg acgatcgtga
      841 gcggcctcgc gcgcggcatc gacaccgcgg cgcaccgggg cgcgctgcgg gtgggcggcc
      901 gcagcatcgc ggtgctcggc tcgggggtgg acgtggtgta cccgccggag aacgcgcggc
      961 tcgccgagga gatcgtcgcg cgcggggcgc tcgtctcgca gttcgcgccc gggactccgc
     1021 cgctgccgca ccacttcccg acgcgcaacg cggtgatcgc ggcgctgtcg ctcgcggtgg
     1081 tggtggtgga ggcggcggag cgcagcggct cgctgatcac cgcgcgtctc gccgccgagc
     1141 tcggccgcga ggtgctcgcg acccccggtc gcgtgaccgc gtcggagagc cggggggcca
     1201 accggctgat ccaggacggg gccgcgctgg ccctgggctg ggaggacgtg gtggccgcgc
     1261 tgcccgagcg gtggaaggcc tgtgtcgaca cgcgcgagag aatcgaggcg acgggcgccc
     1321 gcggcgcggc ggccgcgtcc ggggcgggcc ggccgagcgc ggcgacccgg cgggtgctcg
     1381 cgctgctcgg ggaggacccg ctcgagatcg accacgtgat cgagcggagc gggctcgggg
     1441 ccggcccggt gtcggcggcc ctgctcgatc tcgagctgga ggggcgggtg cgtcagatcg
     1501 aggggaagcg attcgtgcga gtcggacggg gataggaggc actgccggtg gcgaagcgaa
     1561 gcttggtggt ggtcgagtca cccacgaagg tcaagacgat ccagaagtat ctggactcga
     1621 agtacatcgt caaggcctcg atgggccacg tccgcgacct gcccaagtcc aagctcgggg
     1681 tggacgagaa gaagggcttc aagccggagt acaaggtgct gccggccaag aagaaggtcc
     1741 tcgacgagct gaagaaggcg gccgagaagg tggacgcgct ctacatcgcg accgacccgg
     1801 accgggaagg ggaggcgatc ggctggcatc tggcccagga gctcggggtc agcaagagca
     1861 agacccaccg gatcatgttc aacgagatca ccgagcgcgc ggtcaaggcc gcgttcctgc
     1921 acccgggcaa gatcgacacc aacaaggtca acgcccagca ggcgcgccgg gtgctcgacc
     1981 gtctggtcgg ctacaagctc tcgcccctgc tctgggaaaa gatccgccgt gggctctccg
     2041 ccgggcgggt gcagtcggtc gcggtgcgcc tcatcaccga gcgcgagcgc gagatcgccg
     2101 cgttcgtgcc ggtagagtac tggtcgctcc acgcgcgcct caagggcaag aacccgcccg
     2161 acttcaccgc caccctcaag gaagtgaagg accagaaggc ggacctgccg gacgaggcgt
     2221 ccaccatgac cgtgatgacc gcgctccacg ggcaggcgtg gaccgtgaag tcggtgaccc
     2281 gcggggagcg gcgccggaac ccggcgcctc cgttcatcac ctccaccctc cagcaggacg
     2341 cgagccgcaa gctccacttc agcgccaaga agaccatgat gctggcccag cagctctacg
     2401 agggcgtcga gctcggcgag gaaggggcgg tcggcctcat cacctacatg cggaccgacg
     2461 cggtgcgggt ggccgcggag gcgcaggagg aggcgcggca gtgggtggcc gggcgcctcg
     2521 gccaggagta cctgccggac gcgccaccca cctacaaggc caagaagagc gcgcaggagg
     2581 cccacgaggc ggtgcggccc agctccgtcg cgcgcgagcc caaggcggtg gcccgcttcc
     2641 tcagcaagga tcagctcgcc ctctaccgca tcatctggga gcgcttcctg gccagccaga
     2701 tgctgcccgc ggtctacgac acggtcgccg ccgacatcgt ggcgggcgac tgcctgttcc
     2761 gcgcccaggg ctcgaccctc aagttcgccg gcttcaccgc ggtctacatc gagtcgcgcg
     2821 aggccaccga cgtggagccc gaggaggagg cggaggcggt ggtgccgccg ctcacggtgg
     2881 gcgagatcct gaagctcctg gccctggacc ccaagca
//
LOCUS       DATMCV010000187         6840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_276750, whole genome shotgun sequence.
ACCESSION   DATMCV010000187 DATMCV010000000
VERSION     DATMCV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276750"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            598..951
                     /locus_tag="VNU03_04380"
     CDS             598..951
                     /locus_tag="VNU03_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73721.1"
                     /translation="MECLEHGVAVRMAESLCEGVRYMLDSPVSLVLAEAGMLRMAGPD
                     QARLFDAVAPGVPVVLAVDAGARVEDRVELELKGFHVVSRPFALRDLLAKIERPAKAA
                     PARGEARAQVEALCR"
     gene            963..1175
                     /locus_tag="VNU03_04385"
     CDS             963..1175
                     /locus_tag="VNU03_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73722.1"
                     /translation="MGKRWVFGVLSLAIAACLIGLLAFTGESVHRVAPRATIHHTISN
                     TAPEARPATEPEMGPTYQGNGLMLTY"
     gene            1320..1706
                     /locus_tag="VNU03_04390"
     CDS             1320..1706
                     /locus_tag="VNU03_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWP73723.1"
                     /translation="MASKLYVGGLSYSTTSETLREYFAQCGTVESASVITDKFSGQSR
                     GFGFVEMATAEEAQRAISELNGKDLDGRKITVNVSNPRTPGSGGGRGPGGPRGGGGGR
                     GPGGPRGGGGDRGAYGVPPSKKPFRW"
     gene            complement(1792..3633)
                     /locus_tag="VNU03_04395"
     CDS             complement(1792..3633)
                     /locus_tag="VNU03_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HWP73724.1"
                     /translation="MIDVKTADRELQTYLRPQTFPVAIRMLRADEPIPERARRPARDF
                     KKLSMSCQVIDMSRRYGWTIALTREDHICSLGITAIGFDKPLPIYNVGTLCEGMYTET
                     KEAGQRSEAAIDKFAPGEYHCVLVAPLDRATFEPHVVCVYANPAQVMRLTQAALWKRG
                     GRLTSSFEGRAVCADIIVTTMKTGEPQVILPCSGDRIFGQTQDHEMAFSIPWSRMEEM
                     VEGLRGTHNGGIRYPITQFMEYEAKLPPRYMEVNKLWDAEKGKTALTNRDRVVAAYKR
                     SFSDRVPVYPIVASFAGTLDGLSIEEYCTNPTRAISAMMNYYERYQPDVVLAYNDLAK
                     EAEAFGCGVKYSDYVVPSIETHLLEDKADLAKLSMPDPYKTARLPGFLEQCEALVKAA
                     PPAATGAVAVGPWTIAMLLRNPEMMLLDTFEDPQFIHDLMRLTTDFCKLWGDAIAKTK
                     IGLSFSEPTASISLVSPDNYREFIAPYHKELVDHFKAKKVGVTAHICGVTYPIFEDLI
                     GCGFTTISFDLDQQSDPTLHVDQLERFVQVAKGRAVAIGNVDATMFEKATRPQIEAEV
                     GRCIDTAARQSGFILSTSCEIPPRSNPEIVKWFMDAAHDLGRYERIL"
     gene            3718..4008
                     /locus_tag="VNU03_04400"
     CDS             3718..4008
                     /locus_tag="VNU03_04400"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HWP73725.1"
                     /translation="MARVRIIQWRDLPSLVEAEEGGETARVPLSQRFQDLIDAVAMRE
                     GATESEAYLAGWAPGPESERPGGAQAVAQAVADELEAGFQDLVMRRFLGPPR"
     gene            4067..4270
                     /locus_tag="VNU03_04405"
     CDS             4067..4270
                     /locus_tag="VNU03_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73726.1"
                     /translation="MKAAELVVACFLLFGTCLVWPLLAIANRPVLVAGVPALVLYLFV
                     VWAGMVVVLIALARRLRPPEDET"
     gene            4267..>6840
                     /locus_tag="VNU03_04410"
     CDS             4267..>6840
                     /locus_tag="VNU03_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWP73727.1"
                     /translation="MSGLSPTALLSIVLGYLAFLFLVASVAEALAPRLGRPRLRTLTY
                     VLAASVYCTAWTFYGSVGLAANRGLEFLTIYLGPACVALLWPLLLRKLVRVSKEQRIT
                     SISDFISSRYGKSAALGTLVAALVVCGMIPYIALQLKAVSASFKMILREEAVLEVFDP
                     TLVVAVTLALFGILFGARNLDFTKRQTGLMTAVAVESVVKLVAFLLVGVYVTWGLFGG
                     FADLFGQVARHPEWSKLLTLDQATTASYPRWTAMLLISMLAVMFLPRQFHVLVVQNPR
                     ERDVNAVSWSFPLYLLLINVFVLPIAFAGLIVFPHASAQADGFILRLPLFFDNQLISV
                     LVFLGGFSAATAMIVVDSLALSKMITNDIILPMLLRQRRMEDIYWITLFYTRLAMLAV
                     VGLGFLWARMEHGQLLLVEMGLLSFIAVAQCGPAILLGLYWRRGNRRGAFAGVSAGFF
                     VWFYTLIIPALGQEGLFGPSFLADGPLGFGILRPTAFLGLEGLDTISHGVFWSLFFNV
                     GLFVMVSLLTEQDADDRAQAAAFVGVAGEDKPAPGSPAILSAPEIERLVHHYVGDEDA
                     EDIVRELFGSKAPADLSVPELLEMRIRFERLLAASLGAAAARMIVEDHFTISKEEAQQ
                     LVTSFQRMQESLRVTEEEVKRGERLLASVVESVDDCIFTADTTGRLVTMNPAGRRLLG
                     YEAWEVSRLRYPDLLAGEERGSAAPLVGALEAGRGWGGQVMGRTAQGRAFPAHVAMTS
                     LFDDRGHRMGTVGVLRDLTEQVETQRRLIHREKLASLGEMAAGVAHEIRNPLGGIKMA
                     TNLLSSPEVDGSPLSQEMTRSILSGIVEIETIINSLLDWTRDARLERGDYDLLRILDP
                     VV"
BASE COUNT          963 a         2291 c         2398 g         1188 t
ORIGIN      
        1 caccagcgca cgagacggtg tgagtggcgg atgggcgagc agcgatacct gtgccgacac
       61 ctgttcctgc tggcaatccg cgcattccct ctcgtgggcg atcagcaggg ccagctcggt
      121 cacgccggtc cagccgcgcg aagcgcggga ggacacctcg ggagctccac cgcaattcat
      181 cggcgatgct cgggagcgag caggataacg gcacggacaa agacacacga atccaaccca
      241 atcacggttc caaccgaggg cattgtctgc cctccctcta ccgccgctca gccctctccg
      301 tctgtcggcg tgaacgggta ccttaccaca aaccgggccc gtcgctcggc gcgcttcgac
      361 catggtctcc agcgccgcct gctgtcactc gatcgggcag aacgcccgac cgtcgtcacg
      421 tccgtgcgga aggcccggcc caagtgcgcg tctatgcagg ggtttgacgg tcagattctg
      481 ctggcacgcg cgttgaatct atccacttgg ttcgactcgt agagaactac aaaaggggga
      541 gacatgattc gggtactgac gatcgatggg aatcgcagcc gcgctcagga gctcgccatg
      601 gagtgtctgg agcacggtgt cgccgtgcgc atggcggagt cgttgtgcga gggcgtgcgc
      661 tacatgctgg actcgccggt ctcgctggtg ctcgcggagg cgggcatgct gcggatggcc
      721 ggcccggacc aggcgcgtct gttcgacgcg gtggctcccg gtgtgccggt ggtgctcgcg
      781 gtggacgcgg gcgcgcgggt ggaggatcga gtggagctcg agctgaaggg ctttcacgtg
      841 gtgagtcgcc ccttcgcgct gcgggacctg ctcgcgaaga tcgagcggcc ggccaaggcc
      901 gcgccggcgc gcggtgaggc gcgggcgcag gtcgaggcgc tgtgccgata ggacgcgcga
      961 tcatgggcaa gcgctgggtg ttcggagtcc tgagcttggc gatcgcggcc tgcctcatcg
     1021 gtctgctcgc cttcaccggc gaatccgttc accgcgtggc tccgcgggcc acgattcatc
     1081 acaccatctc gaacacggca ccggaggcgc ggcccgccac cgagccggag atgggaccta
     1141 cctaccaggg caacggtctg atgctgacct actgaggggc ccgccgtggc ggacgcggag
     1201 gtcctggtgt acactcggga ccgatgcgcc gccggcggcg gcgccctcgc ctccgatatt
     1261 ggctcttccg agtattgggg cgcgtacgaa gcggaagagt gaccgaagga gaaaccacga
     1321 tggcttcgaa actgtacgtg gggggtctgt cctattccac gaccagcgag acgctaaggg
     1381 aatacttcgc gcagtgcggt accgtggagt cggcttccgt gatcacggat aagttctcgg
     1441 gccagtcgcg ggggttcggg ttcgtcgaga tggccaccgc cgaggaagcg cagcgcgcca
     1501 tctccgagct gaacggcaag gatctggacg gccggaagat caccgtcaac gtctcgaacc
     1561 cgcgtacgcc gggctcgggc ggcggtcgcg gccccggcgg gccccgcggc ggcggcggcg
     1621 ggcgcggtcc gggtgggcct cgcgggggcg gcggggatcg cggcgcctac ggcgtgccgc
     1681 cctcgaagaa gccgttccgc tggtagcgcc ccacactcgc gggcgagaga tcaaaaaggg
     1741 cgcggtgatc gtcaccgcgc cctttctctt gtcctcgcgc tccccgcgct tctacaagat
     1801 ccgctcgtag cggcccaggt cgtgggccgc gtccatgaac cacttcacga tctcgggatt
     1861 cgaccgcggc gggatctcgc acgaggtgga caggatgaag cccgactgcc gggccgcggt
     1921 gtcgatgcag cggcccacct ccgcctcgat ctgcggccgg gtcgccttct cgaacatcgt
     1981 cgcatcgacg ttgccgatcg ccaccgcgcg gcccttggcc acctgcacga accgctcgag
     2041 ctggtccacg tgcagggtcg gatccgactg ctggtcgaga tcgaacgaga tggtggtgaa
     2101 gccacagccg atcaggtcct cgaagatggg gtaggtgacc ccgcagatgt gggcggtgac
     2161 gccgaccttc ttggccttga agtggtccac cagctccttg tgatagggcg cgatgaactc
     2221 ccggtagttg tcgggagaga ccaggctgat cgacgcggtg ggctcggaga acgagagccc
     2281 gatcttggtc tttgcgatcg cgtcgcccca gagcttgcag aagtcggtgg tgagccgcat
     2341 gaggtcgtgg atgaactggg gatcctcgaa ggtgtcgagc agcatcatct ccgggttgcg
     2401 cagcagcatc gcgatcgtcc acggccccac cgccaccgcg ccggtggccg cgggcggggc
     2461 cgccttcacg agggcctcgc actgctcgag gaagccgggt aggcgcgcgg tcttgtacgg
     2521 atcgggcatc gagagcttcg ccagatccgc cttgtcctcg agcaggtggg tctcgatgga
     2581 gggcaccacg tagtccgagt acttcacccc gcagccgaag gcttccgctt ccttggccag
     2641 atcgttgtag gcgagcacca cgtccggctg gtagcgctcg tagtagttca tcatcgcgct
     2701 gatggcccgg gtcgggttcg tgcagtactc ctcgatgctg agcccgtcga gcgttcccgc
     2761 gaaggaggcc acgatcgggt agaccggcac ccggtccgag aacgagcgct tgtaggcggc
     2821 taccacccgg tcccggttgg tcagggcggt cttgcccttc tccgcgtccc agagcttgtt
     2881 cacctccatg tagcgcggag ggagcttggc ctcgtactcc atgaactggg tgatcgggta
     2941 gcggatgccc ccgttgtggg tgccgcgcag cccctccacc atctcctcca tccggctcca
     3001 cggaatggaa aacgccattt catgatcctg ggtctggccg aagatgcgat cgcccgagca
     3061 cgggaggatg acctgcggct cgccggtctt catggtggtc acgatgatgt ccgcgcacac
     3121 cgcccggccc tcgaacgacg aggtgagccg gccgccgcgc ttccagagcg cggcctgggt
     3181 cagccgcatc acctgggcgg ggttggcgta gacgcacacc acgtgcggct cgaacgtggc
     3241 gcggtcgagg ggagccacca ggacgcagtg gtactcgccg ggggcgaact tgtcgatggc
     3301 ggcctccgag cgctggcccg cctccttggt ctcggtgtac atgccctcgc acagggtgcc
     3361 cacgttgtag atgggcagag gcttgtcgaa gccgatcgcg gtgatgccga gcgagcagat
     3421 gtggtcctcg cgggtgaggg cgatggtcca gccgtagcgg cgggacatgt cgatcacctg
     3481 gcagctcatg gagagcttct tgaaatcgcg cgcgggccgg cgggcgcgct cgggaatcgg
     3541 ctcgtcggcc cggagcatgc ggatggcgac cgggaaggtc tgcggcctca agtaggtctg
     3601 cagctcgcgg tccgccgtct tcacgtcgat catgaatggt ctcctcagtg acggaggcta
     3661 cggggggcct tcggggttgt caagggagcg ggccgccttc cgctacaatg tgcagccatg
     3721 gcgcgcgtgc ggatcatcca gtggcgtgac ctgccgtccc tcgtcgaggc ggaggagggc
     3781 ggcgagacgg cgcgtgtccc cctgtcccag cggttccagg atctcatcga cgcggtggcg
     3841 atgcgggaag gcgccaccga gagcgaggcc tacctcgcgg ggtgggcccc gggtcccgag
     3901 agcgaacggc cgggcggggc gcaggcggtg gctcaggcgg tcgcggacga gctcgaggcc
     3961 gggttccagg acctggtgat gcggcgcttc ctgggcccgc cgcgctaggc ggggggcctc
     4021 ggaggtcgcc gcgccgcggg ctccggcgct ataatccgcg cagccaatga aggccgcaga
     4081 gctggtggtt gcctgcttcc tcctgttcgg gacctgcctc gtctggcccc tgctcgccat
     4141 cgccaaccgg ccggtgctcg tggccggggt gcccgccctg gtgctctacc tgttcgtggt
     4201 gtgggccggg atggtggtgg tcctgatcgc cctggcccgt cggctccggc cgcccgagga
     4261 cgagacgtga gcggcctgtc gccgaccgcg ctgctcagca tcgtcctcgg ctacctggcc
     4321 ttcctcttcc tggtcgcctc ggtcgcggag gccctcgcgc cgcggctcgg ccgcccccgg
     4381 ctgcgaaccc tgacctacgt gctcgccgcc tccgtctact gcaccgcgtg gacgttctac
     4441 ggcagcgtgg ggctcgccgc gaatcgcggg ctcgagttcc tcaccatcta cctgggcccc
     4501 gcctgcgtgg ccctgctctg gccgctcctg ctccgcaagc tggtccgggt ctccaaggag
     4561 cagcgcatca cctcgatctc cgacttcatc tcgagccgct acggcaagtc cgccgcgctc
     4621 ggcaccctgg tggcggcgct ggtggtctgc gggatgatcc cgtacatcgc actgcagctg
     4681 aaggcggtct cggcctcgtt caagatgatc ctgcgcgaag aggcggtgct cgaggtcttc
     4741 gaccccacgc tggtggtggc ggtgaccctc gccctcttcg gcatcctgtt cggggcgcgg
     4801 aacctcgact tcaccaagcg ccagaccggg ctcatgaccg cggtcgcggt cgagtcggtg
     4861 gtgaagctgg tcgcgttcct gctggtcggg gtgtacgtga cctgggggct cttcggcggc
     4921 ttcgcagacc tgttcgggca ggtggcccgg catccggagt ggtccaagct cctgaccctc
     4981 gatcaggcga ccaccgcgtc ctacccgcgg tggaccgcga tgctcctgat ctccatgctg
     5041 gcggtgatgt tcctgccgcg gcagttccac gttctcgtgg tccagaatcc gcgcgagcgc
     5101 gacgtgaacg cggtctcctg gtcgttcccg ctgtacctgc tcctgatcaa cgtgttcgtg
     5161 ctccccatcg cgttcgcggg cctcatcgtg ttcccgcacg cgtcggctca ggccgacggc
     5221 ttcatcctgc gcctgcccct cttcttcgac aaccagctca tctcggtgct ggtgttcctg
     5281 ggcggcttct cggccgccac cgccatgatc gtggtggact ccctcgccct gtcgaagatg
     5341 atcaccaacg acatcatcct gcccatgctg ctgcgccagc ggcggatgga ggacatctac
     5401 tggatcaccc tcttctacac gcgcctggcc atgctcgcgg tggtgggcct gggcttcctc
     5461 tgggcgcgga tggagcacgg ccagctgttg ctggtcgaga tggggctgct gtcgttcatc
     5521 gcggtggccc agtgcggtcc cgccatcctg ctcggcctct actggcggcg cggcaaccgc
     5581 cgcggcgcct tcgcgggcgt ctcggccggc ttcttcgtgt ggttctacac cctgatcatc
     5641 cccgcgctcg gtcaggaggg cctgttcggt ccctccttcc tggccgacgg tccgctcggc
     5701 ttcggcatcc tgcggcccac cgcgttcctc ggcctggaag gcctcgacac catcagccac
     5761 ggcgtcttct ggtcgctctt cttcaacgtg gggctcttcg tgatggtgtc gctgctcacc
     5821 gagcaggacg cggacgaccg ggcccaggcc gccgccttcg tgggggtggc cggcgaggac
     5881 aagccggccc cgggctcgcc cgcgatcctc tccgcgcccg agatcgagcg gctggtgcac
     5941 cactacgtgg gcgacgagga cgcggaggac atcgtgcgcg agctgttcgg gagcaaggcg
     6001 cccgccgacc tgtcggtgcc ggagctcctg gagatgcgca tccgcttcga gcggctgctc
     6061 gccgcctcgc tgggcgcggc cgccgcccgc atgatcgtcg aggaccactt cacgatctcg
     6121 aaggaggagg cccagcagct cgtcacctcc ttccagcgga tgcaggagtc cctccgggtc
     6181 accgaggagg aggtcaagcg cggggagcgc ctgctcgcct cggtggtgga gagcgtcgac
     6241 gactgcatct tcaccgcgga caccaccggg cggctcgtca ccatgaaccc ggccgggcgg
     6301 cggctcctcg gttacgaggc gtgggaggtg agccggctgc gctatccgga cctcctggcc
     6361 ggcgaggagc gcgggagcgc cgcgcccctc gtgggcgcgc tcgaggcggg ccggggctgg
     6421 ggcgggcagg tgatgggccg caccgcgcag ggtcgcgcgt tcccggccca cgtggcgatg
     6481 acgtcgctgt tcgacgaccg cgggcaccgc atggggacgg tcggcgtgct gcgcgacctg
     6541 accgagcagg tggagacgca gcggcggctc atccaccgcg agaagctggc ctccctcggc
     6601 gagatggcgg cgggagtggc ccacgagatc cgcaatccgc tcggcggcat caagatggcc
     6661 accaatctcc tctcctcgcc ggaggtcgac ggcagcccgc tgtcccagga gatgacccgg
     6721 tccatcctct ccggcatcgt ggagatcgag accatcatca acagcctgct ggactggacg
     6781 cgcgacgcgc ggctcgagcg cggtgactac gacctgctcc gcatcctcga cccggtggtg
//
LOCUS       DATMCV010000188         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_209569, whole genome shotgun sequence.
ACCESSION   DATMCV010000188 DATMCV010000000
VERSION     DATMCV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209569"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VNU03_04415"
     CDS             complement(<1..368)
                     /locus_tag="VNU03_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HWP73728.1"
                     /translation="MGALALLNGLFRRHVGGAQRPPFHDIDATYPSLRLLDRNHAVIR
                     EELLAVLSDQERIPRYHEISRAETYISGTVDPGKSWRVFMLAHVSGGIPTNQAQSPAP
                     ARCFARSPGCCRHSSRSSIRA"
     gene            complement(368..1069)
                     /locus_tag="VNU03_04420"
     CDS             complement(368..1069)
                     /locus_tag="VNU03_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016392907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73729.1"
                     /translation="MLSLEDVHTYYGEAHILQGVSLSVGDGEVVTMIGRNGAGKTTTL
                     LSIMGIARARRGAVRMGGQDITHLETHEIVRRGIGWVPEERRVLPNLTVLENLRLGTM
                     AAPGADAERRLEEALEYFPRLRERIAQKGRFLSGGEQQMLAIARGLVSHPRIMLVDEP
                     TEGLAPLLVRSLTEILREINRRGTTILLVEQTLEVAMALSHRLYVMDQGRIQFEGTPE
                     ALRRDPTIQQRFLSV"
     gene            complement(1072..1827)
                     /locus_tag="VNU03_04425"
     CDS             complement(1072..1827)
                     /locus_tag="VNU03_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73730.1"
                     /translation="MTAPDGVLLRTERLTRSFGSLTAVNGVSLTVRKGELRSIIGPNG
                     AGKTTFFRLVSGEMEPSSGRVLFQETDITGLPQHRVCRLGIAKSYQITNIFPHLSVLE
                     NVRVAVQGYARSFNFWSRADQIAGCRERAAEILDTVSLARQADRPAAHLSHGEKRHLE
                     IGIALASDPALLLLDEPTAGMSPEETDETMVLIRELARGRTVVLVEHKMKLVMGISDR
                     VTVLHQGAVLADGTPEEIRANRIVQETYLGAAR"
     gene            complement(1824..>2036)
                     /locus_tag="VNU03_04430"
     CDS             complement(1824..>2036)
                     /locus_tag="VNU03_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWP73731.1"
                     /translation="FDFFEEYCARLPDFTSEDFAMHRVILGAGVVIMEGLTNLAALPR
                     RRVEFAAPFYKIAGTEGAPARFFATV"
BASE COUNT          298 a          710 c          730 g          298 t
ORIGIN      
        1 gcccggatcg aggatcgaga agaatgcctg cagcagcccg gggatctggc gaagcagcgc
       61 gctggtgcgg gggactgcgc ctggttggtc gggatgccgc cgctcacgtg ggccagcatg
      121 aagactcgcc agctcttgcc cgggtccacc gtccccgaga tgtaggtctc ggcccgcgag
      181 atctcgtggt agcgggggat gcgctcctga tccgacagca ccgccaggag ctcctcgcga
      241 atgaccgcgt ggttgcgatc caggagccgg agcgacgggt aggtcgcgtc gatgtcgtga
      301 aagggcgggc gctgcgctcc acccacgtgc cggcggaaca ggccgttcag gagcgccagc
      361 gcgcccacta gacgctcagg aagcgctgct ggatcgtggg atcccggcgc agcgcctcgg
      421 gggtgccctc gaactggatg cggccctggt ccatcacgta gaggcggtgc gagagggcca
      481 tcgcgacctc gagggtctgc tcgacgagga ggatggtggt gccgcgccgg ttgatctcgc
      541 gcaggatctc ggtcaggctc cgcaccagca gcggggcgag cccctcggtc ggctcgtcca
      601 ccagcatgat ccgcggatgg gagacgaggc cgcgggcgat ggccagcatc tgctgctcgc
      661 cgcccgagag gaagcggccc ttctgggcga tgcgctcgcg caggcgcggg aagtactcga
      721 gcgcctcctc gagccggcgc tccgcgtcgg cccccggcgc ggccatggtg ccgagccgca
      781 ggttctccag cacggtcagg ttcggcagga cccgccgctc ctccggcacc cagccgatgc
      841 cccgccgcac gatctcgtgg gtctccaggt gggtgatgtc ctggccgccc atgcgcaccg
      901 ccccgcgccg cgcccgcgcg atgcccatga tcgagagcag ggtcgtggtc ttcccggccc
      961 cgttgcgccc gatcatggtg accacctcgc cgtcgcccac cgacagcgag acgccctgga
     1021 ggatgtgggc ctcgccgtag taggtgtgca cgtcctccag cgagagcagg ctcaccgcgc
     1081 cgccccgagg tacgtctcct gcacgatccg gttcgcgcgg atctcctccg gcgtgccgtc
     1141 ggccagcacc gcgccctggt gcagcacggt gacccggtcc gagatcccca tgaccagctt
     1201 catcttgtgc tcgaccagca ccacggtgcg cccgcgggcc agctcgcgga tcagcaccat
     1261 cgtctcgtcg gtctcctcgg ggctcatgcc cgcggtgggc tcgtccagca ggagcagcgc
     1321 ggggtccgag gccagcgcga tgccgatctc cagatgccgc ttctcgccgt gggagagatg
     1381 cgcggcgggg cggtcggcct ggcgcgcgag ggaaaccgtg tcgaggatct cggccgcgcg
     1441 ctcgcggcag cccgcgatct ggtcggcgcg cgaccagaag ttgaacgagc gcgcgtaccc
     1501 ctgcaccgcg acccgcacgt tctcgagcac cgagaggtgc gggaagatgt tggtgatctg
     1561 gtaggacttg gcgatgccga gccggcacac ccggtgctgg ggcagcccgg tgatgtcggt
     1621 ctcctggaac agcacgcgcc cggaggacgg ctccatctcg cccgagacca ggcggaagaa
     1681 ggtggtcttg cccgccccgt tcggcccgat gatcgagcgc agctcgccct tgcgcaccgt
     1741 gaggctcacc ccgttcaccg cggtcaggct gccgaaggat cgggtgagcc gctcggtccg
     1801 cagcagcacc ccgtcggggg cggtcatacc gtcgcgaaga atcgcgcggg ggctccctcg
     1861 gtgccggcga tcttgtagaa gggggccgcg aactccacgc gccggcgcgg cagcgcggcc
     1921 aggttggtga gcccttccat gatgaccacg cccgcgccca ggatcacccg gtgcatcgcg
     1981 aagtcctccg aggtgaaatc gggcaggcgc gcgcagtact cctcgaagaa gtcgaa
//
LOCUS       DATMCV010000189         3159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_411437, whole genome shotgun sequence.
ACCESSION   DATMCV010000189 DATMCV010000000
VERSION     DATMCV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411437"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1193
                     /locus_tag="VNU03_04435"
     CDS             174..1193
                     /locus_tag="VNU03_04435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP73732.1"
                     /translation="MKLGIYLNAQHPAGDDPARRFAETLEQARLIRSLGFDSIWGGEH
                     HATPGFHYFPLLPMLQRLAAEVPGLEIGTNLVLLPLHNPVEIAEVGAFLDVLTGGRFL
                     LGVGLGYRPEEFAIFGVPMAERVSRLAEGVEIIRRLWTEDKVTHRGRHWTFENLTIRP
                     RPIQSPRPPILVGSQVPAGIARAARIADGWLVVPLPRVDEFAAQARAFTAARAAEGRT
                     DDAQICRLLEVVCAPDEETAIRRAAPYLLDKYAAYFSWGLEGVTLDPGAPPEAQLRGL
                     AANRFAIGSPAQVVDALLAQHAAGATHLTMRVSWPGMPQADVLAGIELLGRRVLSEVR
                     RRTAA"
     gene            1246..2325
                     /locus_tag="VNU03_04440"
     CDS             1246..2325
                     /locus_tag="VNU03_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HWP73733.1"
                     /translation="MRVPAGKIEEQLVAVLGAWGMSAEHAAVTAQMMVETDLRGVDSH
                     GISMLPTYDKEFRAGRLNMRPQWKVVRDRPSMARIDADRSLGHPISAHAMNLAVDKCR
                     ATGVGVVAVFNSHHFGAAGCYAKIAADRGVIGMVTSATRGVTLVPTFAAEPIMGTNPL
                     AFAAPARRNAPFQLDMATTTVAAGKVKVYKLNHKPVPSGWVVDGDGRAVTDETEAFRH
                     VFERPEGGITPLGGGRELGGHKGYGLAVMVHILGGTLAGASFSPLRNRTQRDSDPHNI
                     GHFFMAIDPGAFRDEGEFESDLDDVIDVLHGAKRADPAQPVLVAGDPERLTRTERLEQ
                     GVPIPDDLMEQLRGVAASARVPFVL"
     gene            complement(2327..>3159)
                     /locus_tag="VNU03_04445"
     CDS             complement(2327..>3159)
                     /locus_tag="VNU03_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWP73734.1"
                     /translation="MRSRTAALGPFEEVAAVADHRVPVTGGEITVRVYQPAGVGPHPV
                     LVFYHGGGWVIGDLYTHDGICRSIVNAAGCAVASVDYRLAPEFKYPTPVEDSYAGLQW
                     VAANATRIGLDPARIAVGGDSAGGNLAAVMALLARDRHGPRILLQVLIYPVTNHDFGT
                     ASYRENATGFLLSTEDMRWFWRHYLSREEQGREPSVSPLLAKSLADLPPALVVTAGCD
                     PLRDEGEAYAVRLRDAGVAVTLTQYSGMFHGFLRMTRILDQSRVLLDEVAGALRKSLS
                     "
BASE COUNT          450 a         1116 c         1153 g          440 t
ORIGIN      
        1 caaccgcctg ttcatggccg cgagccagtc gctctacgcg gtctacaccg ccacgcaggg
       61 cgccgccccc gggtagacgg gagccgaaag cggggtcagg tcgtacattc cgacaacgtg
      121 ggccgacatt gtcgcaatgt aagacctgac cccatccgcc atccacgcca gccatgaagc
      181 tcgggatcta tctcaacgcg cagcacccgg cgggcgatga tccggcgcgc cggttcgcgg
      241 agacgctgga gcaggcgcgc ctgatccgct cgctggggtt cgactcgatc tggggtgggg
      301 agcaccacgc cacgcccggc ttccactact tcccgctgct gccgatgctg cagcgattgg
      361 ccgcggaggt gccggggctc gagatcggca ccaacctcgt gctgctgccg ctccacaacc
      421 cggtggagat cgcggaggtg ggggccttcc tcgacgtgct caccggcggc cgcttcctgc
      481 tgggcgtggg tctcggctat cggcccgagg agttcgccat cttcggtgtg ccgatggcgg
      541 agcgggtgag ccgtctcgcg gagggcgtcg agatcattcg gcgactctgg accgaggaca
      601 aagtcaccca ccgcggccgg cactggactt tcgagaacct cacgatccgg ccgcggccga
      661 tccagtcgcc gcgcccgccg atcctagtcg gctcgcaggt gcccgcgggc atcgcgcgcg
      721 ccgcgcgcat cgcggacggc tggctggtgg tgccgctgcc gcgcgtcgac gagttcgccg
      781 cgcaggcgcg ggccttcacc gcggcgcgcg ccgccgaggg gcggaccgat gacgcgcaga
      841 tctgccggct gctcgaggtg gtctgtgcgc ccgacgagga gaccgcgatc cgccgcgccg
      901 cgccctacct gctcgacaag tacgcggcct acttctcctg gggcctcgag ggcgtcacgc
      961 tggacccggg cgctcctccg gaagctcagc tccgcggtct cgcggccaac cgcttcgcga
     1021 tcggctcgcc cgcccaggtc gtcgacgcgc tgctggccca gcacgcggcc ggcgccaccc
     1081 acctgacgat gcgtgtgagc tggccgggga tgccgcaggc ggacgtcctc gccggcatcg
     1141 agctcctggg tcgccgggtc ctttccgaag tccgtcgccg taccgcggcc taggcgggcc
     1201 gccgaatgtc gttttgtaag acctgacccc aaccgaggag atctcatgcg agtgcctgcg
     1261 gggaagatcg aggagcagct ggtggcggtg ctcggggcgt gggggatgtc ggcggagcac
     1321 gcggcggtca cggcccagat gatggtcgag accgatctgc gcggggtgga ctcgcacggc
     1381 atctcgatgc tgccgaccta cgacaaggag ttccgcgccg gccggctcaa catgcggccc
     1441 cagtggaagg tcgtgcgcga ccggccctcg atggcgcgca tcgacgcgga ccgctcgctc
     1501 ggccatccga tctcggccca cgcgatgaac ctggcggtgg acaagtgccg ggccaccggc
     1561 gtcggcgtgg tcgcggtgtt caactcgcat cacttcggcg cggcgggctg ctacgcgaag
     1621 atcgcggcgg accgcggggt gatcggcatg gtcacctcgg ccacccgcgg ggtcacgctg
     1681 gtgccgacct tcgcggcgga gccgatcatg ggcacgaacc cgctcgcctt cgcggcgccc
     1741 gcgcgccgca acgccccgtt ccagctcgac atggcgacca cgacggtggc ggccggcaag
     1801 gtgaaggtct acaagctcaa ccacaagccg gtgccgtcgg gctgggtggt ggacggcgac
     1861 ggacgcgcgg tgaccgacga gacggaggcc tttcgccacg tgttcgagcg gccggagggt
     1921 ggcatcaccc cgctcggggg cggccgcgag ctgggcgggc acaagggcta cggcctcgcg
     1981 gtgatggtgc acatcctggg cgggacgctg gccggcgcgt cgttctcgcc gctgcgcaac
     2041 cggacccagc gcgactcgga tccgcacaac atcggccact tcttcatggc catcgacccg
     2101 ggcgccttcc gcgacgaggg cgagttcgag agcgatctcg acgacgtgat cgacgtgctg
     2161 cacggggcca agcgcgcgga cccggcccag ccggtgctgg tggcgggcga ccccgagcgg
     2221 ctcacccgca cggagcggct cgagcagggc gtgccgatcc cggacgacct gatggagcag
     2281 ctgcgcgggg tggcggcctc cgcgcgggtg cccttcgtgc tctaggctac gagagggact
     2341 tgcgcagggc tcccgcgacc tcgtcgagca gcacgcgcga ctggtcgagg atgcgggtca
     2401 tgcgcaggaa gccatggaac atcccggagt actgggtgag cgtcaccgcc acccccgcgt
     2461 cgcgcagccg caccgcgtag gcctcgccct cgtcccgcag cggatcgcag cccgcggtca
     2521 ccacgagcgc gggcggcaga tcggccaggc tcttggccag gagcggggag accgacggct
     2581 cgcgcccctg ctcctcgcgc gacaggtagt ggcgccagaa ccagcgcatg tcctcggtgc
     2641 tcagcaggaa cccggtggcg ttctcgcgat aggaggcggt gccgaagtcg tggttggtga
     2701 ccgggtagat cagcacctgc agcaggatcc gcggcccgtg gcgatcgcgg gccagcagcg
     2761 ccatcaccgc ggccaggttc ccgcccgcgc tgtcgccgcc gaccgcgatg cgcgccggat
     2821 cgagcccgat gcgcgtggcg ttggccgcca cccactggag ccccgcgtag gagtcctcga
     2881 ccggcgtcgg gtacttgaac tcgggcgcga gccggtagtc caccgacgcg accgcgcagc
     2941 cggccgcgtt gacgatggac cggcagatgc cgtcgtgggt gtagaggtcg ccgatgaccc
     3001 agccgccgcc gtggtagaag accagcactg gatgcgggcc gacgccggcg ggctggtaca
     3061 cgcgcaccgt gatctcgccg ccggtgaccg gcacgcgatg gtcggccacc gcggccacct
     3121 cctcgaacgg gccgagcgcg gcggtgcgcg agcgcatgg
//
LOCUS       DATMCV010000190         5989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_9748, whole genome shotgun sequence.
ACCESSION   DATMCV010000190 DATMCV010000000
VERSION     DATMCV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9748"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1229
                     /locus_tag="VNU03_04450"
     CDS             240..1229
                     /locus_tag="VNU03_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HWP73735.1"
                     /translation="MPGAYWCMVEDDRQGAPKKVMAEVMGEASRLAGQAGAQAEAVWV
                     TDKATPEGIKQLGEWGAKRIWLLENAAFAPYRGEVWTPVVADLAGKESPQAIFAPVSA
                     RQREFMARLAARLGAGLSADSTGMALDSGKLVATRPIYAGKLLSKMTWAKTPWMATLR
                     PNVFRPAESQAGASAAVEKPAATIPGETMKLVERRQEASTGLPELTEAEIVVSGGRGL
                     KGPENFVILEELAKVLGAAVGASRAAVDAGWRPHRFQIGQTGRTISPKLYLGFGISGA
                     IQHLAGMRTSKVICAINKDPEAPIFKIADYGIVGDLFEVAPLLTQEFKHLLDK"
     gene            1231..2388
                     /locus_tag="VNU03_04455"
     CDS             1231..2388
                     /locus_tag="VNU03_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWP73736.1"
                     /translation="MQLDLTPEQTMIRDLARGFADKEVRPIADAIDRDARFPTETVGR
                     MAELGLMGIAVPEAYGGSGSDTVAYALAVEELARGCASHAVIMSVNNSLYCDPVLRHG
                     TEAQKARFLTPFASGRKLGCYALTEPEAGSDASNQTTLARTDGDAYVLDGRKVFVTNG
                     REAAAALVFAQTDRAAGHRGISAFLVEKGTPGFTVVKTEDKLGIRASDTAELLFEGCR
                     VPASQRLGEVGQGFRIALGALDGGRIGIAAQAVGIATAAYEAALAYARERKSFGVPIG
                     QHQMVQWMLADMATTIEAARLLTLRAAWRKDSGAPYGHEAAMAKLFASETAMRVTTDA
                     IQVHGGYGFIKEYQVERHFRDAKITQIYEGTSQIQKLVVARHLLADAGSAA"
     gene            2403..4055
                     /locus_tag="VNU03_04460"
     CDS             2403..4055
                     /locus_tag="VNU03_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWP73737.1"
                     /translation="MADDKERWERESYRPFVRRTPERDVPYETLSGIPVQPLYTPEDL
                     VGWRYEDKLGYPGEFPFTRGPYPSMYRGRLWTMRMFAGFGRPEDTNARFKYLLQQGQT
                     GLSTAFDMPALMGYDADHPRARGEVGREGVSISTLADFEILFRDIPLDQVTTSMTINC
                     TASVALAMYLALADKQGVGWDKIGGTMQNDMLKEFIAQKEWISPPESAVRVVVDTIEF
                     CSRHVPRFNPVSISGYHIREAGSTAVQELAFTLADGFGYVAAAVERGLDVDDFAPRLS
                     FFFDIHNDFFEEIAKLRAARRIWATVMRDRFRARKPESMRLRTHTQTAGVSATAQQPL
                     NNVARVALQALAAVLGGAQSLHTNSYDEVLALPTEEAVTVALRTQQIIADETGVALTA
                     DPLGGAYFLERLTDQMEAQAMDYIRKIDELGGIVRAIDLGYPQQEIADAAYRYQLMDD
                     RGEKAVVGVNKYVMPEEKPVEYLRIDPAIEQEQIERVGRVKASRDAARVERRLKQLAE
                     ACRDNVNVMPVLIDAVKDYVSLGEISDVYRRMFGLYREPIIF"
     gene            4068..5135
                     /locus_tag="VNU03_04465"
     CDS             4068..5135
                     /locus_tag="VNU03_04465"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HWP73738.1"
                     /translation="MTEPIRINRSATRKPKPKDADLGFGQIFTDHMFLADFQEEKGWY
                     DPRIEPYGPLSLDPATAVLHYAQAIFDGLKAFRGVDGKIRLFRPQKHVERMNNSARRL
                     CIPPIDPDFALQSLVRLVDVDRDWVPSTVGTSLYIRPTVIASEPFLGVRPAKSYIYFV
                     ILSPVGAYYPEGMAPVKILVVDKYVRAVDGGLGAAKTSGNYAASLYASDEAKHEGFTQ
                     VLWLDGVHRKYLDEVGTMNIMVKIGDEIITPPLTGTILPGVTRDSTLALLRKWGLKVS
                     ERQVSIDEVVEAHGRGKLEEVWGTGTAAVISPVGELAYKGKKMVINGGKIGPLTQKLY
                     DAIVAIQYGKAADPDGWTLAI"
     gene            5161..5658
                     /locus_tag="VNU03_04470"
     CDS             5161..5658
                     /locus_tag="VNU03_04470"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP73739.1"
                     /translation="MIASVAEFARYWDGVRRRTWAAVDRVEPGLLDWAPRPGEMTCGE
                     IVRHLAGAERFFVTKVVEDRFTEDLSPGAALDHAETRARLEGVHREEMARLATLDDAV
                     LASPRRDLDGGTVKAWRFLMAMVEHEVHHRSQLDSYLSLVGVEPPQIYGWRMEDVVAK
                     VAGAR"
     gene            5655..>5989
                     /locus_tag="VNU03_04475"
     CDS             5655..>5989
                     /locus_tag="VNU03_04475"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HWP73740.1"
                     /translation="MTDCVFCKIRDGQIPSLKIFEDDRTLAFMDINPVSHGHCLVIPK
                     AHAASLFEAEVEDLQATIAAAQQVARAIREALAPDGLNMLQANGAAAFQSVPHFHLHL
                     IPRWANDGKG"
BASE COUNT          994 a         2028 c         2134 g          833 t
ORIGIN      
        1 cgccggaacg gttgtccgcc gactggggag tgcgggagcg cgcgccggaa cggttgtccg
       61 ccgactgggg agtgcgggag cgcgcgccgg aacggttgtc cgccgactgg gggagtgcgg
      121 gggccatttc tgggcccccg cagctgaaaa gcttaaagaa ctaaacggac gaaacaggca
      181 cacgaaaagc ttaaagaact aatcggacga aacgggcaca acaagaaaag ggatcaaaca
      241 tgccaggcgc gtactggtgc atggtggaag acgatcggca gggcgcaccc aagaaggtga
      301 tggccgaggt gatgggcgag gcctcgcgcc tggccgggca ggcgggggcg caggccgagg
      361 cggtgtgggt gaccgacaag gcgactcccg aggggatcaa gcagctcggg gagtggggcg
      421 ccaagcggat ctggctgctc gagaacgcgg cgttcgcgcc gtaccgggga gaggtgtgga
      481 cgccggtggt ggcggatctg gccggcaagg agtcgccgca ggccatcttc gcgccggtct
      541 ccgctcgcca gcgcgagttc atggcgcggc tcgccgcgcg gctcggggcc gggctctccg
      601 ccgactcgac cggcatggcg ctcgactcag gcaagctggt ggccacccgg ccgatctacg
      661 cgggcaagct gctgtcgaag atgacgtggg ccaagacccc gtggatggcc acgctgcggc
      721 ccaacgtgtt ccgtcccgcg gaatcgcagg cgggcgcgag cgctgcggtg gagaagccgg
      781 ccgcgacgat cccgggcgag accatgaagc tggtggagcg gcgccaggaa gcctccaccg
      841 gtcttcccga gctgaccgag gccgagatcg tggtctccgg gggccgcggg ctcaagggcc
      901 cggagaactt cgtcatcctg gaagagctgg ccaaggtgct gggcgcggcg gtcggcgcct
      961 cgcgtgccgc ggtggacgcg ggctggcggc cgcatcgctt ccagatcggc cagaccgggc
     1021 gcacgatctc gcccaagctc tacctgggct tcggcatctc cggcgcgatc cagcacctgg
     1081 ccggcatgcg gacctccaag gtgatctgcg cgatcaacaa ggacccggag gcgccgatct
     1141 tcaagatcgc cgactatggc atcgtcggcg acctcttcga ggtcgccccc ctgctcaccc
     1201 aagagttcaa acacctcctg gacaagtaaa gtgcagctag acctgacgcc agagcagacc
     1261 atgatccggg acctggcccg cggcttcgcg gacaaggagg tgcgcccgat cgcggacgcg
     1321 atcgatcggg acgcgcgctt cccgaccgag accgtggggc gcatggccga gctgggtctg
     1381 atggggatcg cggtgccgga ggcctacggt ggcagcggca gcgacacggt ggcctacgcc
     1441 ctggcggtcg aggagctcgc gcggggctgc gcctcccatg cggtgatcat gtcggtcaac
     1501 aactcgctct actgcgatcc ggtgctgagg cacggcaccg aggcgcagaa ggcgcgcttc
     1561 ctcaccccgt tcgcgtccgg acgcaagctc ggctgctacg cgctcaccga gccggaggcg
     1621 ggctcggacg cgagcaacca gaccacgctg gcgcgaacgg acggagacgc ctacgtgctg
     1681 gacggccgca aggtgttcgt caccaacggg cgcgaggcgg ccgccgcgct cgtgttcgcc
     1741 cagaccgacc gggccgcggg acaccgcggc atcagtgcct tcctggtgga gaagggcacg
     1801 ccgggcttca cggtggtgaa gaccgaggac aagcttggca ttcgcgcgtc ggacaccgcc
     1861 gagctgctct tcgaggggtg ccgggtgccc gcgtcccagc ggctcggcga ggtcgggcag
     1921 gggttccgga tcgccctcgg cgccctcgac ggcggccgca tcgggatcgc ggcccaggcg
     1981 gtcggcatcg cgaccgcggc ctacgaggcc gcgctcgcgt acgcgcgcga gcggaagagc
     2041 ttcggggtgc cgatcggcca gcaccagatg gtgcagtgga tgctcgcgga catggccacc
     2101 accatcgagg cggcccgcct gctcaccctg cgcgcggcgt ggcgcaagga ttcgggcgcg
     2161 ccctacggcc acgaggcggc catggccaag ctcttcgcct ccgagaccgc gatgcgggtg
     2221 accaccgacg cgatccaggt gcacggcggc tatggcttca tcaaggagta ccaggtcgag
     2281 cgccacttcc gcgacgccaa gatcacccag atctacgagg gcacgtccca gatccagaag
     2341 ctggtcgtcg cccgtcacct gctcgccgac gccgggtcgg cggcctgagg gagcgcgagt
     2401 ccatggccga cgacaaggag cgctgggagc gggagagcta ccggccgttc gtgcggcgca
     2461 cgccggaacg ggacgtgccg tacgagacgc tctcgggcat cccggtgcag ccgctctaca
     2521 caccggagga tctggtcggg tggcgctacg aggacaagct cggctacccg ggcgagttcc
     2581 cgttcacgcg cggaccctat ccctcgatgt accgcgggcg gctctggacc atgcggatgt
     2641 tcgccggctt cggccgcccg gaggacacca acgcccgctt caagtacctc ctgcagcagg
     2701 gccagaccgg gctctccacc gccttcgaca tgcccgccct catgggctac gacgcggacc
     2761 acccgcgggc ccgcggcgag gtgggacgcg agggggtgtc gatctccacc ctcgccgact
     2821 tcgagatcct gttccgcgat atcccgctcg accaggtgac cacgtcgatg accatcaact
     2881 gcaccgcgtc ggtcgcgctc gccatgtacc tggcgctcgc cgacaagcag ggggtgggct
     2941 gggacaagat cggcggcacc atgcagaacg acatgctcaa ggagttcatc gcccagaagg
     3001 agtggatctc gccccccgag tccgcggtgc gggtggtggt cgacaccatc gagttctgct
     3061 cccggcacgt gccgcgcttc aacccggtct cgatctccgg ctaccacatc cgggaggccg
     3121 gctcgaccgc ggtgcaggag ctggccttca ccctggccga cggcttcggc tacgtggccg
     3181 ccgcggtcga gcgcggtctc gacgtcgacg acttcgcccc gcgcctgtcg ttcttcttcg
     3241 acatccacaa cgacttcttc gaggagatcg cgaagctgcg ggcggcccgg cggatctggg
     3301 ccaccgtgat gcgcgaccgc ttccgggccc ggaagccgga gtcgatgcgc ctccgcacgc
     3361 acacccagac cgcaggggtg tcggccaccg cccagcagcc gctcaacaat gtggcgcggg
     3421 tggcgctgca ggctctcgcc gcggtgctgg gcggggccca gtccctccac accaactcct
     3481 acgacgaggt gctcgccctc cccaccgagg aggcggtgac ggtggccctg cgcacccagc
     3541 agatcatcgc cgacgagacg ggcgtggcgc tgaccgcgga tccgctgggc ggcgcctact
     3601 tcctggagcg cctcaccgat cagatggaag cgcaggcgat ggactacatc cgcaagatcg
     3661 acgagctggg cggcatcgtg cgcgccatcg acctcggcta cccgcagcag gagatcgcgg
     3721 acgcggccta ccgctaccag ctcatggacg atcgcgggga gaaggcggtg gtgggcgtca
     3781 acaagtacgt gatgcccgag gagaagccgg tcgagtacct gcgcatcgac cccgctatcg
     3841 agcaggagca gatcgagcgg gtggggcggg tgaaggcctc gcgcgacgcg gcccgggtcg
     3901 agcggcgcct caagcagctc gcggaggcct gtcgcgacaa cgtcaacgtc atgccggtgc
     3961 tgatcgacgc ggtgaaggac tacgtatcgc tgggagagat ctcggacgtg taccgccgga
     4021 tgttcgggct gtaccgagag cccatcatct tctgaggaga gtgagccatg actgagccga
     4081 tccggatcaa ccgctccgcc acccgcaagc ccaagccgaa ggacgccgac ctgggattcg
     4141 gacagatctt caccgaccac atgttcctgg ccgacttcca ggaggagaag ggctggtacg
     4201 acccgcgcat cgagccgtac gggccgctct ccctcgaccc ggccaccgcg gtgctgcact
     4261 acgcccaggc catcttcgac gggctcaagg cgttccgcgg ggtggacggc aagatccgcc
     4321 tgttccgtcc gcagaagcac gtggagcgga tgaacaactc ggcgcggcgc ctctgcatcc
     4381 cgccgatcga cccggacttc gcgctgcagt cgctcgtccg gctggtcgac gtggaccggg
     4441 actgggtgcc gagcacggtg ggcacctcgc tctacatccg ccccaccgtc atcgcgagcg
     4501 agcccttcct gggcgtgcgg ccggccaagt cctacatcta cttcgtcatc ctgtccccgg
     4561 tgggcgccta ctaccccgag gggatggccc cggtgaagat cctggtggtc gacaagtacg
     4621 tccgcgcggt cgacggcggc ctgggcgcgg ccaagacctc cggcaactac gcggccagcc
     4681 tctacgcgag cgacgaggcg aagcacgagg gcttcaccca ggtgctctgg ctcgacgggg
     4741 tgcaccgcaa gtatctcgac gaggtcggca ccatgaacat catggtgaag atcggcgacg
     4801 agatcatcac cccgccgctc accgggacga tcctgcccgg cgtcacccgc gactcgaccc
     4861 tggccctgct gcgcaagtgg gggctcaagg tgagcgagcg ccaggtgtcg atcgacgagg
     4921 tggtggaggc gcacgggcgc ggcaagctgg aggaggtgtg ggggaccggg accgccgcgg
     4981 tcatctcgcc ggtgggcgag ctcgcctaca agggcaagaa gatggtgatc aacgggggca
     5041 agatcgggcc gctcacccag aagctctacg acgccatcgt ggcgatccag tacggcaagg
     5101 ccgccgaccc cgatggctgg acgctcgcga tctgagcggg cggcggccgc ggcccccacg
     5161 atgatcgcct ccgtcgccga gttcgcccgg tactgggacg gagtccgccg tcgcacctgg
     5221 gccgcggtcg accgggtcga gcccgggctg ctcgactggg cgccgcggcc cggggagatg
     5281 acgtgcgggg agatcgtccg gcacctcgcg ggcgccgagc gcttcttcgt taccaaggtg
     5341 gtggaggacc ggttcaccga ggacctgagc cccggggccg ccctggacca cgcggagacc
     5401 cgcgcccggc tcgagggcgt gcaccgggag gagatggcgc gcctggccac gctcgacgac
     5461 gcggtgctcg cgagcccgcg ccgcgatctc gacggcggca ccgtgaaggc gtggcgcttc
     5521 ctgatggcga tggtcgagca cgaggtgcat caccggagcc agctcgacag ttacctgtcc
     5581 ctggtcggag tggagccgcc ccagatctac gggtggcgga tggaggacgt ggtggcgaaa
     5641 gtggcgggtg cgcgatgacc gactgtgtct tctgcaagat ccgagatggc cagatcccgt
     5701 cgctcaagat cttcgaggac gaccgcacgc tcgccttcat ggacatcaac ccggtcagcc
     5761 acgggcactg cctggtgatc ccgaaggccc acgcggccag cctgttcgag gcggaggtgg
     5821 aggacctgca ggcgaccatc gccgcggccc agcaggtggc gcgggccatc cgcgaggcgc
     5881 tcgcgccgga cgggctcaac atgctccagg ccaacggcgc cgccgccttc cagtcggtgc
     5941 cgcacttcca cctgcacctc atcccgcgct gggccaacga cggcaaggg
//
LOCUS       DATMCV010000191         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_542760, whole genome shotgun sequence.
ACCESSION   DATMCV010000191 DATMCV010000000
VERSION     DATMCV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542760"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1101
                     /locus_tag="VNU03_04480"
     CDS             121..1101
                     /locus_tag="VNU03_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP73741.1"
                     /translation="MRVVASLVLLATLCTASPALPQTAPAPFGPAPFGEVAHHVVRVD
                     GLRIHYVTAGSGEPVVLIPGWPEMSWIAWRKVIPLLVQAGRQVYVLEPRGFGDSDKPQ
                     GGYDLDTAARDLHGFLAATGLGHPAGVDIVAHDVGTWIAHAHAAAYPGEVKRLVLTES
                     NVPGITPPPPAGIPSEAVNLKSWQFAFNRLEDLPEVLVQGRERAYLTWLFATKTTRPY
                     AIEPAALDEYVRVFSAPGTARAGFAWYRANFSPEGLAQAKVRAAKRLPMPVLALGASD
                     GVGDALRATVATLGDHVKGGAIGQGCGHFLPSECPDDLVAAILAFWRDPR"
     gene            1153..1761
                     /locus_tag="VNU03_04485"
     CDS             1153..1761
                     /locus_tag="VNU03_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWP73742.1"
                     /translation="MGDGEERTVIETGHAAIPGSDAETRRAEIELARRARRGDAAAFD
                     ELVRRFHRPVYRFCWRLVRSPDAEDLAQDTFVRAFVHFERFDPQRPVLPWLIAIARRL
                     CLDLFRRRKVMARVDTLPVTGPPPPGPEGEASLREQLSRLERALDDLAEGPRVAIVLF
                     HIEEMSYRDIAAALEVPMGTVMTWLHRGRAQLKRALESESGT"
     gene            1758..>2038
                     /locus_tag="VNU03_04490"
     CDS             1758..>2038
                     /locus_tag="VNU03_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73743.1"
                     /translation="MSERPDENGRLTSLLQALRGPEPSAAFLARARRRYLVAIEARDR
                     RQVLTGLVAALVGLAVVAILLGPTIEPAALLAPLAGVAADLARWTTGVGV"
BASE COUNT          286 a          751 c          679 g          322 t
ORIGIN      
        1 ccgggtatga ttcgcctggt cgcccgcgcc ccccgtggaa agcacgttct gacccttgcc
       61 gtgaacgatc ccgggctgcg agcgtacgtg tttaccttcg gcccctgagg agagtaaccg
      121 atgcgtgtcg tggcttcgct cgtcctgctc gccaccctct gcaccgcgag cccggccttg
      181 ccgcagacgg cgcccgcgcc gttcggcccc gcgccgttcg gtgaagtcgc ccaccacgtg
      241 gtccgggtgg acgggctccg catccactat gtgaccgcag gctcgggcga gccggtcgtc
      301 ctgatccccg gctggcccga gatgagctgg atcgcctggc gcaaggtcat ccccttgctc
      361 gtccaggccg gacgccaggt ctacgtgctc gaaccgcgcg gctttgggga cagcgacaag
      421 ccccagggcg gctacgacct cgacaccgcg gcccgcgacc tgcacggctt cctggccgcc
      481 accgggctcg gccatccggc cggcgttgac atcgtcgcgc acgatgtcgg gacctggatc
      541 gcccatgccc acgccgcggc ctatccaggt gaggtcaagc gcttggtcct gaccgaatcc
      601 aacgtgcccg gcatcacgcc gcccccgccc gcgggcatcc cgagtgaagc ggtgaacctc
      661 aagagctggc aattcgcctt caatcggctg gaggacctgc ccgaggtcct cgtgcagggg
      721 cgcgagcgcg cctatctcac ctggctcttc gcgacgaaga ccacgcgacc ctacgcgatc
      781 gagcctgccg cgctcgacga atatgtccgt gtcttctccg cgccgggaac cgcgcgggcc
      841 ggcttcgcct ggtaccgcgc caacttcagc cccgaagggc tggcccaggc caaggtgcgc
      901 gccgccaagc ggctgccgat gccggtgctg gcgctcggcg ccagcgacgg cgtcggcgac
      961 gccctgcgcg cgacggtcgc gaccctcggc gaccacgtga agggcggcgc catcggccaa
     1021 ggctgcggcc actttctgcc ctccgagtgc cccgacgacc tcgtcgccgc catcctcgcc
     1081 ttctggcgtg acccgcgctg acgaacaacc tcccggtgcc tgaaataacc ctcgggcatc
     1141 gttcgtactc tcttgggaga tggcgaggag cgtacggtca tagagaccgg tcatgcagca
     1201 atcccaggct cggatgcgga aacccggcga gcagagatcg agctggcgcg tcgcgcccgc
     1261 cgcggcgacg cggcggcctt cgacgagctg gtccggcgct ttcaccggcc ggtctaccgg
     1321 ttctgctggc gactggtccg atcgcccgac gccgaggatc tcgcccagga cacgttcgtg
     1381 cgcgccttcg tccacttcga gcggttcgat ccgcagcgcc cggtcttgcc ctggctcatc
     1441 gccatcgcac ggcggctctg cctggacctg ttccggcggc ggaaggtgat ggccagggtg
     1501 gacacgctgc cggtcaccgg ccccccgccg ccaggtcccg agggcgaggc ctcactgcgg
     1561 gagcagcttt cacgtctcga gcgagctctc gacgatctgg ccgaggggcc gagagtagcc
     1621 atcgtcctct tccacatcga ggagatgtcg taccgggaca tcgccgcggc cctcgaggta
     1681 ccgatgggca cggtgatgac gtggctccat cgcggccgtg cccagctcaa gcgggcgctg
     1741 gaaagcgaat ccgggacatg agcgagcgac ccgacgagaa cgggcggctt accagcctgc
     1801 tccaggcgct tcgcgggccg gagccatcgg ccgcgtttct cgcccgcgcg cggaggcgct
     1861 atctggtggc gatcgaggcg cgcgaccgac gtcaggtcct cacgggactc gtggccgcgc
     1921 tcgtcggcct ggcggtggtc gccattctcc tgggtccgac catcgagccg gcagctcttc
     1981 tcgccccgct cgcgggagta gcggccgacc tcgccagatg gacaaccggc gtcggcgt
//
LOCUS       DATMCV010000192         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_9994, whole genome shotgun sequence.
ACCESSION   DATMCV010000192 DATMCV010000000
VERSION     DATMCV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9994"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VNU03_04495"
     CDS             <1..622
                     /locus_tag="VNU03_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HWP73744.1"
                     /translation="RPPRPVAAPTVLLAPTWGPQSTLHVCGEPLIAALLGAGIRVILR
                     PHYQTARLAPRLVEGLVARFGADPGFRHVDRMEESDSLLDSDLLISDWSAMAIEYALG
                     LGKPVLFVDVPPRVRNPRYGELGLEPMEMRIRHELGTVLPLDRLADAPRYVADLRRDA
                     ESLRKRSVALREEWAFNFGRSVEAGAREIARLAEQQRGRGAAGRPR"
     gene            619..>2020
                     /locus_tag="VNU03_04500"
     CDS             619..>2020
                     /locus_tag="VNU03_04500"
                     /inference="COORDINATES: protein motif:HMM:NF023321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HWP73745.1"
                     /translation="MTLRRRLLRWAHWFTAVHAGLLGLIGLRYLWLYLRLSPTVAGVY
                     APLAYVGHIAVLAYLPIFLLVLPVILLLPRPRVVVPLGVVLAGASAGFLMLDSLVFAE
                     NRYHLGILTATLLAPQTWLFLGIYFALGVAIEGMLARAVWRRTETAPRRHVGRYLALA
                     LVLCFAAANVMHAWFEARYEVSVTSFTRYLPLYFPLRDSKMQVRLGLVDRVAARERGV
                     AAALGRPTAGELQYPLRPLTCSPPRPLPNVLIIVIDAMRADALTPEYAPRMSEFARGA
                     TRFASHYSGGNSSRAGMFSLFYGVPATYWDAFAGFARPPVVMDLFRQHEYQLGIFASS
                     PLYRIVNLDRTALARIPNLRLETKSPYPGSSGRDRTLTEEWFRWLDQRDPARPFLGFL
                     YYNAVVSIEPPDDYGHMFQPPQNPTKQQRLAARYLSAVHFVDGLVGQVLDDLRRRKLL
                     DDLVVLITSDHGLEFDE"
BASE COUNT          229 a          777 c          684 g          330 t
ORIGIN      
        1 ccggccgccg cggcccgtcg ccgcgcccac cgtgctgctc gccccgacgt ggggtccgca
       61 gtcgaccctg cacgtgtgcg gtgagcccct gatcgcggcc ctgctcggcg cggggatccg
      121 cgtgatcctg cggccgcact accagaccgc gcggctcgcc ccgcggctcg tcgagggcct
      181 ggtcgcccgg ttcggggcgg acccgggctt ccggcacgtg gaccggatgg aggagagcga
      241 ctcgctgctc gactcggatc tcctgatcag cgactggtcc gcgatggcga tcgagtacgc
      301 gctcggcctg ggcaagccgg tgctcttcgt cgatgtgccc ccccgggtgc ggaacccgag
      361 gtacggcgag ctcggcctcg agccgatgga gatgcgcatc cgtcacgagc tcgggaccgt
      421 cctgccgctc gaccggctcg ccgacgcgcc ccgctacgtg gccgatctgc ggcgggacgc
      481 ggagtcgctc cgcaagcgca gcgtcgcgct ccgggaggag tgggcgttca acttcggccg
      541 gagcgtcgag gcgggcgcgc gggagatcgc gcgcctcgcc gagcagcagc ggggccgcgg
      601 cgcggccggc cggccgcgat gaccttgcgg cgccggctgt tgcgatgggc gcactggttc
      661 accgcggtgc acgcgggcct gctcgggctc atcggcctgc gctacctctg gctctacctg
      721 cggctctccc cgacggtggc cggggtctac gcgccgctcg cctacgtcgg ccacatcgcg
      781 gtgctcgcgt acctgccgat cttcctgctg gtgctgccgg tcatcctgct gctgccgcgg
      841 ccgcgcgtgg tggtgccgct cggggtggtg ctggccggcg cgtcggccgg cttcctgatg
      901 ctcgacagcc tggtcttcgc ggagaatcgc tatcacctcg gcatcctgac cgcgaccctg
      961 ctggccccgc agacgtggct cttcctcggc atctacttcg cgctcggcgt ggccatcgag
     1021 ggcatgctcg cgcgcgcggt gtggcgccgc accgagaccg ccccccgccg gcacgtcggc
     1081 cgttacctgg ccctcgccct cgtgctgtgc ttcgcggccg cgaacgtcat gcacgcgtgg
     1141 ttcgaggccc gctacgaggt ctcggtgacc tcgttcaccc gctacctgcc gctctacttt
     1201 ccgctgcgcg actccaagat gcaggtgcgg ctgggcctcg tcgatcgcgt cgccgcccgc
     1261 gagcgcgggg tcgcggccgc gctcggccgg cccacggcgg gggagctcca gtacccgctc
     1321 cgccccttga cctgctctcc gccccggccg ctgcccaacg tgctcatcat cgtcatcgac
     1381 gccatgcggg cggacgccct cactccggag tacgcccccc gcatgagcga gttcgcccgg
     1441 ggggcgaccc gcttcgcctc ccactacagc gggggcaatt cctcccgggc cggcatgttc
     1501 tccctcttct acggtgtgcc cgcgacctac tgggatgcct tcgccggctt cgcccggccc
     1561 cccgtggtga tggacctgtt ccgccagcac gagtaccagc tcgggatatt cgcgagctcg
     1621 ccgctgtacc ggatcgtcaa cctcgaccgc acggccctcg cccggatccc gaacctgcgc
     1681 ctcgagacca agtcgcccta cccgggctcg agcgggcggg accgcaccct gaccgaggag
     1741 tggttccggt ggctcgacca gcgggatccc gcgcgtccat ttctcgggtt cctctactac
     1801 aacgcggtgg tgtccatcga gccgcccgac gactacggcc acatgttcca gccgccgcag
     1861 aacccgacca aacagcagcg cctggccgcg cgctacctga gcgcggtgca cttcgtggac
     1921 ggtctggtcg ggcaggtgct cgacgacctg cgccgccgca agctgctcga cgacctggtc
     1981 gtgctcatca cctccgacca cggcctggag ttcgacgaga
//
LOCUS       DATMCV010000193         3095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_744667, whole genome shotgun sequence.
ACCESSION   DATMCV010000193 DATMCV010000000
VERSION     DATMCV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744667"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VNU03_04505"
     CDS             complement(<1..547)
                     /locus_tag="VNU03_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWP73746.1"
                     /translation="MADYVRSRLRQTTHTTRGAVLTGARWLARQEASVLVAALVILLA
                     LFGFIRIAEELGQGQLAHLDEWILHLLRVPGDPHTPIGPPWLLEAARDITPLGGRTML
                     VAVTLFVIGYLALERKFGAMWLVAVATAGGGLLTTGMKDLFGRARPDLVPHLVAVTSP
                     SFPSGHSLLAAVLYLTLGALLA"
     gene            complement(603..1397)
                     /gene="fetB"
                     /locus_tag="VNU03_04510"
     CDS             complement(603..1397)
                     /gene="fetB"
                     /locus_tag="VNU03_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HWP73747.1"
                     /translation="MTSSGAVLDLSLSQLGLALLLVGVIIAISIHQRLGVARDVVVGT
                     LRAMVQLYLVGLILAVVFRAARWYWVLLILVVMTAVAAQAATGRLGKPLPGGGVIAAL
                     ALTVSTAVTLFYVIAVVVRVRPWYEPQYIIPIAGMILGSAMTSAALAGDRLQSDLRAR
                     ADEVEARLALGFSGREAVQPVVRAALRAAMIPTVNGMMTVGLVQLPGMMTGQILAGSS
                     PLLAIKYQLVVVFMQAAATAVAAMLFVRLAVARYLTPAHQFTRSRL"
     gene            complement(1394..2167)
                     /locus_tag="VNU03_04515"
     CDS             complement(1394..2167)
                     /locus_tag="VNU03_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP73748.1"
                     /translation="MAAPPSLRDPLFEIEHLSLAHAGGPNHAPVSILNDVSVGVERGD
                     ALVLVGPSGSGKSTLLRCLNRLVEPTAGRVRFDGRDIRSLDPRELRRRAALVMQTPVL
                     FEGSVRGNLLVRPPGTSGDFSPGRLASALVDVGLDPGLLDRDAATLSGGEKQRVTIAR
                     ALLGDPQALLLDEPTSALDPPNVALVAETVSRLRERYRLTIVAVTHQPELVRRLGGGL
                     LYLVRGEALAYERADDTVSDSRLHAFLSGAQASPPARHP"
     gene            complement(2187..2513)
                     /locus_tag="VNU03_04520"
     CDS             complement(2187..2513)
                     /locus_tag="VNU03_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73749.1"
                     /translation="MTILVSNTLGTALGDFVATSSGLGFERGALVFAGLLALVALAHF
                     FTPLQKSVIFWAAYVLTRPLGATLGDTLTKPHPEGGLGLSRIASSLVIAACIVVGIAA
                     TSAWED"
     gene            complement(2510..2911)
                     /locus_tag="VNU03_04525"
     CDS             complement(2510..2911)
                     /locus_tag="VNU03_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73750.1"
                     /translation="MNKVPHVTLAFWAIKILATTLGETGGDALSMTLELGYAMSTLIF
                     LAFFAVTLVPQVASRRYHAFRYWAVVVATTTVGTTMSDYLDRTLELGYVASSLILCLA
                     GSWPPCSSGAAPRGGSSSTTSPRGRTRSSTG"
BASE COUNT          537 a         1009 c         1171 g          378 t
ORIGIN      
        1 gggccagcaa ggctcccagg gtgaggtaca ggacggcggc caggagggag tgcccgctcg
       61 ggaagctcgg agaggtcacc gcgacgaggt gggggaccag gtcggggcgc gcccggccga
      121 agagatcctt catgccggtg gtgagcaggc ccccacccgc ggtggcgact gccaccagcc
      181 acatcgcgcc aaatttccgc tcgagcgcaa gatatccgat gacgaagagc gtgaccgcga
      241 cgagcatggt ccggccgccg aggggcgtga tgtcccgggc cgcttcgagg agccacggcg
      301 gcccgatcgg cgtgtggggg tcaccgggaa cccggagcag gtggagtatc cactcgtcga
      361 ggtgagccag ctgtccttgg ccgagctcct ccgcgatcct gatgaagccg aagagcgcca
      421 ggaggatgac cagcgcggcc acgagcacgg acgcctcctg ccgggcgagc catcgggccc
      481 cggtcaggac ggcgccgcgg gtcgtgtggg tcgtctggcg caagcggctg cggacgtagt
      541 cggccatcgg gctcgctccc gatccagtgt agccccaagc atcccgcccg tcctgccgag
      601 gtctagagcc gggaccgggt gaactggtgg gccggagtga gatagcgcgc gacggccagc
      661 cggacgaaca gcatggccgc gacggcggtg gccgcggcct gcatgaagac cacgaccagc
      721 tgatacttga tggcgagaag gggagaggag cccgccagga tctgtcccgt catcatgccc
      781 ggcagctgca cgaggccgac cgtcatcatg ccgttgaccg tcggaatcat ggcggcccgg
      841 agcgccgcgc gaaccacggg ctggaccgct tcgcgccccg agaagccgag cgccagccgc
      901 gcctccacct cgtcggcccg ggcgcgaagg tcactctgga ggcggtcgcc ggccagcgcg
      961 gcgctggtca tggcgctgcc caggatcatt cccgcgatcg ggatgatgta ctggggctcg
     1021 taccatgggc gcacgcgtac gacgaccgcg atgacataga acagcgtgac ggcggtcgac
     1081 accgtgagcg ccagcgccgc gatgaccccg ccacccggga gaggcttccc gagccgcccg
     1141 gtcgcggcct gggcggcgac cgcggtcatg acgacgagta tgagcaagac ccagtaccag
     1201 cgggcggcgc ggaagacgac ggccaggatc aggcccacga ggtagagctg gaccatggcg
     1261 cgaagggtac cgacgacgac gtcccgcgcg acgccgagcc gctggtggat ggagatcgcg
     1321 atgatgaccc ccacgaggag caacgcgagc cccagctgcg acagcgacag gtccaggacc
     1381 gcgccgctcg aggtcatgga tgacgggcgg gcggcgaggc ttgggccccg gagaggaagg
     1441 cgtgcagcct cgagtcgctg acggtgtcgt cggctcgctc gtaggcgagg gcctctccgc
     1501 gcaccaggta gagcaagccg cccccgagcc ggcggacgag ctccggctgg tgagtgaccg
     1561 cgacgatcgt gagccggtag cgctcgcgca gtcgcgatac ggtctccgcc accagcgcga
     1621 cgttgggcgg gtcgagggcg gaggtgggct cgtcgaggag cagagcctgc gggtcgccca
     1681 gcagggcgcg ggcgatggtg actcgctgct tctcgccgcc ggacaaggtc gccgcgtccc
     1741 ggtcgagcag cccgggatcg aggccgacgt cgacgagagc cgacgccagc cggcccggcg
     1801 agaaatcgcc ggacgtgccc gggggccgga cgaggaggtt gccccgcacc gagccttcga
     1861 agagcacggg ggtctgcatg accagggccg cgcgccgccg cagctcgcgg gggtcgagcg
     1921 agcggatgtc tcggccgtcg aaccgcacgc gaccggcggt cggctccacc agacggttga
     1981 ggcagcggag gagcgtggac ttgcccgagc ccgacgggcc gacgaggacg agggcgtcgc
     2041 cccgctcgac cccgaccgac acgtcgttga gaatcgagac cggcgcgtgg ttcgggccgc
     2101 ccgcatgggc gagcgagagg tgctcgatct cgaagagcgg gtcccgcagc gatggtgggg
     2161 cggccacggc gcccgcaagc gtaacactag tcctcccagg cggacgtcgc ggctatgccg
     2221 acgacgatgc acgccgcgat gacgagggac gaggcgatgc ggctcagccc gagccctcct
     2281 tcggggtgag gcttggtcag ggtgtcgccc agggtcgcgc cgagagggcg ggtcaggacg
     2341 tacgcggccc agaagatcac gcttttctgg agcggcgtga agaagtgcgc cagcgcgacg
     2401 agggcgagca ggccggcgaa gacgagcgcc ccgcgctcga atccgaggcc cgagctcgtc
     2461 gccacgaagt cgccgagcgc ggtgccgagc gtgttcgaga ccaggatcgt cacccagtag
     2521 aagacctcgt tcggccgcgt ggtgatgtgg tcgaactcga tccgccccgt ggcgcggcgc
     2581 cagacgaaca gggcggccag gaccccgcca aacacaggat gagggaggac gccacgtagc
     2641 cgagctcgag cgtgcggtcg aggtagtccg acatcgtcgt gcccaccgtg gtggtggcga
     2701 ccacgaccgc ccagtaccgg aacgcgtggt accgcctcga tgcgacctga gggaccaacg
     2761 tgaccgcgaa gaaggcgagg aagatcaggg tgctcatcgc gtaaccgagc tcgagggtca
     2821 tcgaaagggc gtcgccgccc gtctcgccca gggtggtcgc gaggatcttg atcgcccaga
     2881 acgccagggt gacgtgcggg accttgttca tgacatcact gaccggcgcg tcggttttcc
     2941 ggccgtcgaa tcgcagggac ggctcctctc gtgattccag gctactcctt gaagaggagg
     3001 acgaagcggc gaccggcgat cggcccgacc gcgagggctc gcaggtgatc cgacgccggg
     3061 aggcgcgaca cgcttcgttc gggcgtcagc agata
//
LOCUS       DATMCV010000194         2966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_78210, whole genome shotgun sequence.
ACCESSION   DATMCV010000194 DATMCV010000000
VERSION     DATMCV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78210"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VNU03_04530"
     CDS             <1..481
                     /locus_tag="VNU03_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HWP73751.1"
                     /translation="DNFITRVFAYRRLTALRYMPPRPRDVVAFHSAHKYLLLAHSPAA
                     YTRLGRLVAAVASEPLAPWLDRYADAFMRALQVTATPRKHVNVLQHIVGFFKSRLSAA
                     EKRELLGVVDDYAGGLVPLIVPITLINHYVARFDTAYVRDQIYLRPHPKELMLRNHV"
     gene            497..>2966
                     /locus_tag="VNU03_04535"
     CDS             497..>2966
                     /locus_tag="VNU03_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWP73752.1"
                     /translation="MPHARPRGERGRVERRASGGERIPWVHVGAFVAGSVLVVLFTAH
                     QIGTERETALLHWKTRIATIAADRARLVSGWLAARRADAEVLAAFPAVRALLAGQGRD
                     EESLARHLTRVASAYGYAGIVVSDAQGRPVARSAADAGAAALEPARAVAEVAASRKPR
                     FELTGTGAGRLLAITVPVFIDGAPAGPDGDPRPLLGVVTLGMKPDASLYPLLADDAEP
                     TRTGETLLVDLDSSPPIYLSPPRQPAPPDSAVVQRSLATLRDLAQAAAQGREPVAETI
                     DYRDVPVVVAVRRVSPTGWTLALKVDRSEVLAEFERSGRLAGAAAAFLLLALAGFLVA
                     LHRARQRARLLQEQMRRERALSNLQGYADTIVASVPAGLLLLSADLHVLSANRAFLEA
                     FRLGEGDVVGRDLKQLVRADRLVRSAGRVLETGAAQPGALYDLYVYARRETKPARVAM
                     TPIRMGDDVPPELLLVIEDLTEEERLQAARQESEERYRDLIQGLDAIVWEADARTLTF
                     SFVSGRAETVLGYPVERWLREPDFWARRIHPEDRDGVMRVYREALAGGRDHEFEYRAV
                     AADGREVWLRDIVHVVRDMDGQPVTLRGLTVDLTELRRSERALHQSEEQLRQAQKMDA
                     VGKLAGGIAHDFNNLLMVIRGDSDLMLRRLPPGHPLRPNAEGVREAADQAATLTRQLL
                     AFSRKQVVAPRLVDLNGIVASIHAMLERLLGETINLVTVTAPDLGGVKADPGQMEQMI
                     LNLCVNARDAMPDGGRLTIRTANVDLDEDAAKEWSDARPGPYVMLEVSDTGGGMDPET
                     RAHLFEPFFTTKEQGKGTGLGLSTV"
BASE COUNT          392 a         1152 c         1039 g          383 t
ORIGIN      
        1 cgacaacttc atcacccgcg tcttcgccta ccgccgcctc accgcgctgc ggtacatgcc
       61 gccgcgaccg cgggacgtcg tcgcgttcca ctccgcgcac aagtacctcc tgctcgccca
      121 cagcccggcc gcctacacgc ggctcggccg cctggtggcc gcggtcgcga gcgagccgct
      181 cgccccctgg ctcgaccgct acgcggacgc gttcatgcgc gcgctccagg taaccgccac
      241 gccgcgcaag cacgtcaacg tcctccagca catcgtgggc ttcttcaaga gccgcctgag
      301 cgcggccgag aagcgcgagc tcctgggggt cgtcgacgac tacgcgggcg ggctcgtgcc
      361 cctgatcgtg ccgatcaccc tgatcaacca ctacgtcgcc cgcttcgaca ccgcctacgt
      421 gcgggaccag atctacctcc gtccccatcc caaggagctg atgctccgaa accacgtctg
      481 agctatcctg gaaagcgtgc ctcatgctcg cccgcgcggg gagcggggac gcgtcgagcg
      541 ccgggcctcc ggcggcgagc gaatcccgtg ggtccacgtc ggcgccttcg tcgccggcag
      601 cgtgctggtc gtcctcttca ccgcccacca gatcggcacc gagcgcgaga ccgcgctcct
      661 gcactggaag acgcgcatcg ccaccatcgc ggccgaccgg gcccggctcg tctcgggctg
      721 gctcgcggcc cggcgcgccg acgcggaggt gctcgccgcg ttccccgcgg tgcgcgcgct
      781 cctcgccggc cagggccggg acgaggagtc gctcgcccgg cacctgaccc gcgtcgcctc
      841 cgcgtacggc tacgcgggca tcgtggtctc cgacgcccag ggccggccgg tggctcgctc
      901 ggcggccgat gcgggcgcgg ccgcgctcga gccggcgcgg gcggtggccg aggtcgcggc
      961 gagccggaag ccgcgcttcg agctgaccgg taccggcgcc gggcggctgc tcgcgatcac
     1021 ggtaccggtc ttcatcgacg gcgcgcccgc cggccccgac ggcgacccgc ggccgctgct
     1081 cggcgtggtc accctgggca tgaagccgga cgcctcgctc tatcccctgc tggccgacga
     1141 cgcggagccc acccgcaccg gggagaccct gctcgtcgac ctggacagct ccccgccgat
     1201 ctacctctcc ccgccacgcc agcccgcgcc gcccgactcg gcggtggtgc agcgctcgct
     1261 cgccacgctg cgcgacctcg cgcaggccgc cgcccagggc cgggagccgg tcgcggagac
     1321 gatcgactac cgcgacgtgc cggtggtggt cgcggtgcgt cgggtgagcc ccaccggctg
     1381 gacgctcgcc ctcaaggtgg accgcagcga ggtgctcgcc gagttcgagc gctcggggcg
     1441 gctcgcgggc gcggcggccg ccttcctgct cctggccctg gccggcttcc tcgtcgcgct
     1501 gcaccgcgcg cgccagcgcg cgcgcctgct ccaggagcag atgcggcggg agcgggccct
     1561 gtccaacctg cagggctacg ccgacaccat cgtggcgagc gtgcccgcgg gcctgctcct
     1621 gctctcggcc gacctgcacg tgctctccgc caaccgcgcc ttcctcgagg cgttccgcct
     1681 cggcgagggg gacgtggtcg gccgcgacct gaagcagctg gtgcgcgcgg accggctcgt
     1741 gcgcagcgcg ggtcgggtgc tcgagaccgg ggcggcccag ccgggcgccc tctacgatct
     1801 ctacgtctac gcgcggcgcg agaccaagcc cgcgcgggtc gccatgaccc cgatccgcat
     1861 gggcgacgac gtcccgcccg agctgctgct ggtcatcgag gacctcaccg aggaggagcg
     1921 cctgcaggcg gcccgccagg agtcggagga gcgctaccgc gacctgatcc agggcctcga
     1981 cgccatcgtg tgggaggcgg acgcgcgcac cctgaccttc tcgttcgtga gcgggcgcgc
     2041 ggagacggtg ctgggctacc cggtcgagcg ctggctgcgg gagcccgact tctgggcccg
     2101 ccgcatccac ccggaggacc gggacggcgt gatgcgcgtg taccgcgagg ccctcgcggg
     2161 cggccgcgac cacgagttcg agtaccgcgc ggtggccgcg gacgggcgcg aggtctggct
     2221 gcgcgacatc gtgcacgtgg tacgcgacat ggacgggcag ccggtgaccc tgcgcggcct
     2281 caccgtcgac ctcaccgagc tccggcggtc ggagcgcgcg ctgcaccaga gcgaggaaca
     2341 gctgcgccag gcccagaaga tggacgcggt gggcaagctg gccggcggca tcgcgcacga
     2401 cttcaacaac ctgctcatgg tcatccgcgg cgacagcgac ctgatgctgc ggcgcctgcc
     2461 gcccggccat ccgctccgcc cgaacgcgga gggggtgcgc gaggcggcgg accaggcggc
     2521 caccctcacc cgccagctcc tcgccttcag ccgcaagcag gtggtggccc cgcgcctcgt
     2581 cgacctcaac gggatcgtgg ccagcatcca cgcgatgctg gaacggctgc tcggcgagac
     2641 catcaacctg gtgacggtga ccgcgcccga cctgggcggg gtcaaggccg accccggcca
     2701 gatggagcag atgatcctga acctctgcgt caatgcgcgc gatgcgatgc cggacggcgg
     2761 ccgcctcacc atccgcaccg ccaacgtgga cctcgacgag gacgcggcca aggagtggag
     2821 cgacgcccgg cccgggccct acgtgatgct cgaggtgagc gacaccggcg gcggcatgga
     2881 tcccgagacc cgcgcgcacc tcttcgagcc cttcttcacc accaaggaac agggcaaggg
     2941 caccgggctc ggcctctcca ccgtct
//
LOCUS       DATMCV010000195         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_477070, whole genome shotgun sequence.
ACCESSION   DATMCV010000195 DATMCV010000000
VERSION     DATMCV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477070"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..518
                     /locus_tag="VNU03_04540"
     CDS             126..518
                     /locus_tag="VNU03_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HWP73753.1"
                     /translation="MPPAFQAIPIFRIFDVDKAKEFYVGFLGFHVDWEARFDETAPLY
                     IQISRDGLLLHLSEHHGDACPGSTVTVQIAGPTGGLESYHREITAKGYRFMRPGIELA
                     PWNARVMEVIDPFGNRLRFNEPVDSAPR"
     gene            complement(487..>2647)
                     /locus_tag="VNU03_04545"
     CDS             complement(487..>2647)
                     /locus_tag="VNU03_04545"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWP73754.1"
                     /translation="VLAVLTGPEAARFCKPYRGVLLHYAGMKTGAMLPLATDRARYVG
                     EPVVAVAAESRALAEDAAARVVVRYEPLPAVLTPEQARERPPLHADLPDNVIYETRLA
                     AGDPAAAFAAAPQRRRFTTGRHTGVPMEPRGLVADFDPFTRALTLWISTQVPHMMQAV
                     VADLFGLPEQRVRVIAPDVGGSFGIKIHVYQDDMAAVALALLLGRPVKFVATRHESFL
                     SDIHAREQRIDVEVAAEADGTLTAIRAAIIAPVGPYSAFPRSSVVEGGQVLRLLPGPY
                     RIRHYEATLAVLAQNKVVTSQYRAVGHPIAAAVTESMVDLVARDLGLDPADVRRRNLV
                     RPEELPYTSAAGNVYDSGSYQAALERLLEAADYVALRREQAAARAAGRHVGIGLSCFI
                     ELTGPGAQFYGIGGAPISGQEGATLRLEPSGAVTALVGVTNQGQGTPLALAQIIADEI
                     GVGVEDITVLSGDTAMGPYGGGTWASRGTPIGGAATLLGARALADRIRRAAAVLLEAA
                     AEDIALDAGRATVRGSDRSLTLREVARAVHFRSSELRGLEPSLEATVHFTNPAPWTFT
                     NGAHLAVVEVDGDTGAIAVRRYVAVDDCGNIVNPALVEGQIAGGIAQGLGGALSEWCV
                     YDEAGQPLATTLMEYAVPTAEVMPAVELHSLESPAPGLPGGFKGAGEAGTTGAPAAIL
                     NAVNDALHPLGVMLTDQPLTPERVWRAISAARSRPAR"
BASE COUNT          334 a          988 c          970 g          355 t
ORIGIN      
        1 atccgctcca tcggcgcgct gtggggcttc ggcgaccgcc gcgagctgac cgacgccggc
       61 gcggacctcc tctgcgagca cccgcgcgcg ctggtggcct gcctgacccg ggccggcggg
      121 tagccatgcc gcctgcgttc caagccatcc cgatcttccg catcttcgac gtcgacaagg
      181 cgaaggagtt ctacgtgggc ttcctcggct tccacgtgga ctgggaggct cggttcgacg
      241 agaccgcgcc gctctacatc cagatctcgc gcgacggcct cctgctccac ctgagcgagc
      301 accacggcga cgcctgcccg ggctcgacgg tgaccgtgca gatcgccggg cccacgggag
      361 gcctggagtc ataccatcgg gagatcaccg cgaagggcta ccgcttcatg cgccccggca
      421 tcgagctggc cccgtggaac gcgcgggtga tggaggtgat cgacccgttc ggcaaccggc
      481 tccgcttcaa cgagccggtc gactccgcgc cgcgctgatc gctcgccaca cccgctccgg
      541 ggtcaggggc tgatcggtga gcatgacgcc cagcgggtgg agcgcgtcgt tcaccgcgtt
      601 caggatcgcg gcgggcgcgc cggtggtgcc ggcctcgccc gcgcccttga agccgccggg
      661 caagccgggc gcggggctct ccagcgagtg gagctccacc gcgggcatga cctccgcggt
      721 cggcaccgcg tactccatca gggtcgtcgc gagcggctgg ccggcctcgt cgtagacgca
      781 ccattccgag agcgcgccgc ccaggccctg ggcgatgccg cccgcgatct gtccttccac
      841 cagcgctgga ttgacgatgt tgccgcagtc gtcgaccgcc acgtagcgcc gcaccgcgat
      901 cgcgccggtg tcgccgtcca cctcgaccac cgcgaggtgc gcgccgttgg tgaaggtcca
      961 cggggcgggg ttcgtgaagt gcacggtggc ctcgaggctc ggctcgagcc cgcgcagctc
     1021 gctggagcgg aagtggaccg cgcgggcgac ttccctcagc gtcaagctcc ggtcgctgcc
     1081 ccgtacggtc gcgcggccgg cgtcgagcgc gatgtcctcc gcggccgcct cgagcagcac
     1141 cgccgccgcg cgccggatcc ggtcggcgag cgcgcgggcg ccgagcagcg tggccgcgcc
     1201 gccgatcggg gtgccccggc tcgcccaggt gccgccgccg tagggcccca tcgcggtgtc
     1261 gccggagagc accgtgatgt cctccacgcc caccccgatc tcgtcggcga tgatctgggc
     1321 gagcgcgagc ggcgtgccct gcccctggtt ggtcaccccg accagcgcgg tcaccgcccc
     1381 cgagggctcg agccgcaggg tggcgccctc ctgccccgag atcggcgcgc cgccgatgcc
     1441 gtagaactgc gcgccgggtc cggtcagctc gatgaagcac gagagcccga tccccacgtg
     1501 gcgcccggcc gcccgcgccg ccgcctgctc ccgtcgcagc gccacatagt ccgccgcctc
     1561 gaggaggcgc tcgagcgcgg cctggtagct gccgctgtcg tagacgttgc ccgccgcgga
     1621 ggtgtagggc agctcctcgg ggcgcaccag gttgcggcgc cgcacgtccg ccgggtcgag
     1681 cccaaggtcg cgcgccacca ggtccaccat gctctcggtg accgcggccg cgatgggatg
     1741 ccccaccgcc cggtactgcg aggtgacgac cttgttctgg gcgagcactg cgagcgtcgc
     1801 ctcgtagtgg cggatgcggt agggcccggg cagcaggcgc agcacctgcc cgccctcgac
     1861 cacgctcgag cgcgggaagg cggagtacgg tcccaccggc gcgatgatgg cggcgcggat
     1921 cgcggtgaga gtgccgtcgg cctcggcggc cacctccacg tcgatccgtt gctcgcgggc
     1981 gtggatgtcg gagaggaacg actcgtggcg cgtcgccacg aacttcaccg gccggcccag
     2041 gagcagcgcg agcgcgaccg cggccatgtc gtcctggtag acgtggatct tgatcccgaa
     2101 gctgccgccc acgtcggggg cgatcactcg cacgcgctgc tcggggaggc cgaagagatc
     2161 ggcgaccacc gcctgcatca tgtggggcac ctgggtcgag atccacagcg tgagcgcccg
     2221 cgtgaacggg tcgaagtcgg ccaccaggcc ccggggctcc atcggcaccc cggtgtggcg
     2281 tcccgtggtg aagcgccggc gctggggcgc ggccgcgaag gcggcggccg ggtcgccggc
     2341 cgcgaggcgc gtctcgtaga tcacgttgtc cgggaggtcg gcgtggaggg gcggccgctc
     2401 ccgcgcctgc tccggggtga gcacggcggg cagcggctcg tagcgcacga ccacccgggc
     2461 cgccgcgtcc tcggcgagcg cgcggctctc ggcggccacc gcgacgaccg gctcgcccac
     2521 gtagcgcgcg cggtcggtgg cgagcgggag catcgcgccc gtcttcatgc ccgcgtagtg
     2581 gagcagcacg ccgcggtagg gcttgcagaa ccgcgccgcc tccggcccgg tcaggaccgc
     2641 caggacc
//
LOCUS       DATMCV010000196         3001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_543176, whole genome shotgun sequence.
ACCESSION   DATMCV010000196 DATMCV010000000
VERSION     DATMCV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543176"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VNU03_04550"
     CDS             <1..371
                     /locus_tag="VNU03_04550"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HWP73755.1"
                     /translation="IQLQNAPDTMSFLELRAYVARLRDGGHRVTRYLVQLYSKLSFPL
                     VHVIMALVAIPFALVSPRSGGRAMGIGVAIVIAVGYWVVHSIALAFAQADLLPAALAA
                     WTANIVFAGIGTALFLSART"
     gene            complement(476..>3001)
                     /locus_tag="VNU03_04555"
     CDS             complement(476..>3001)
                     /locus_tag="VNU03_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWP73756.1"
                     /translation="VFLFVNLSVAARTAPPDVLWELEDQLGLPGRAILEPLVRRLLLP
                     VIGVISIFAGARATGSWPTVIEYLNGTAFGQVDPLFGRDLGFYFFVLPFWRLLHGWGT
                     ALVAGTLVLVAAVYVLQRSLVLTARGPRLAAGARTHLLGLGALLLVIRGIGFWLDRFD
                     LLYSPRGLVFGASYTDVHASLPVLQWLVVLAVLCAAACVFQMFRPGWRFLVAGLVVLV
                     VLWIAGLGVAPALLQTYRVKPNELDFERPYIQHNIRMTRQAYALDRIVEKDFAAEENL
                     NQAALERNNLTIKNVRLWDHRPLLTTYGKLQEIRTYYKFRDVDVDRYMVNGEYRQVTL
                     SARELSYRDLPSRGWINEHLTFTHGYGLVAGPVNRISPEGLPEFFVKDIPPVVAGLPK
                     ITRPEIYYGEIGNEYVFVGTRSQELDYPSGDQNVYTKYEGRGGIPVDSIVRKAAFAAR
                     FGVLTILLSDALGPQSRIMIYRDIATRVQEAAPFLKFDRDPYLVIGADGRLVWMVDGY
                     VTSDRYPYATPVRGFNYIRNSVKATVDAYHGTVSYFIADAEDPLIRTYAKAFPGLLRP
                     MAEMPKDLQPHIRYPEDLFTVQARMYATYHMQDPQVFYNKEDLWVLPRLPQEGRDREM
                     EPYFTVMRLPSEAKEEFVLLAGFNPSGRDNMIALMVARMDPPQYGRLIAYAFPKQKLV
                     FGPRNIQARISQDPIISQQISLWNQQGSRVITGNLLAIPIDQSLLYVQPLYLAATEQG
                     ALPELRRVVVAYGNQIAMEPTLEASLARIFGGRGRPEEAAGRPPERGQTPPPVTAAIT
                     AAVQQAWEAWQRGQEALRKGDWASYGQEQKRIEEALRRLREGR"
BASE COUNT          464 a         1033 c         1043 g          461 t
ORIGIN      
        1 tcatccagct gcagaacgca ccggacacca tgagcttcct cgagctgcgc gcctacgtgg
       61 cgcgcctgcg cgatggcggc caccgcgtca cccgctacct cgtgcagctc tactccaagc
      121 tgtccttccc cctggtgcac gtgatcatgg ccctggtggc gattcccttc gccctggtgt
      181 caccccgcag cgggggccgg gccatgggca tcggggtggc catcgtgatc gcggtgggct
      241 actgggtcgt gcactcgata gccctggcct tcgcccaggc cgacctgctg cccgccgcgc
      301 tcgcggcctg gaccgccaac atcgtcttcg cgggcatcgg caccgccctg ttcctcagcg
      361 ccagaaccta agaggggtca ggtcttacat tccgacattt tttccctcgc tgcccgtctg
      421 cccgcgcccg gcgaaaatgt cggaatgtaa gacctgaccc catcagctga ccccatcagc
      481 ggccttcgcg caggcggcgg agggcctcct cgatgcgctt ctgctcctgg ccgtagctcg
      541 cccagtcgcc cttgcgcagg gcctcctgcc cgcgctgcca cgcctcccag gcctgctgca
      601 cggcggcggt gatcgcggcg gtcaccgggg gcggggtctg gccgcgctcg ggcgggcggc
      661 cggcggcctc ctcgggccgc ccgcgcccgc cgaagatgcg ggcgagcgac gcctcgaggg
      721 tcggctccat cgcgatctgg ttgccgtagg ccaccaccac ccggcgcagc tcgggcagcg
      781 cgccctgctc ggtggcggcc aggtagagcg gctgcacgta gagcagcgac tggtcgatgg
      841 ggatggcgag caggttgccg gtgatcaccc gggagccctg ctggttccac agcgagatct
      901 gctgcgagat gatcgggtcc tggctgatgc gcgcctggat gttgcgcggc ccgaagacca
      961 gcttctgctt ggggaacgcg taggcgatga gacggccgta ctgcggcggg tccatgcggg
     1021 ccaccatgag cgcgatcatg ttgtcgcgcc cgctcggatt gaaccccgcg agcagcacga
     1081 actcctcctt ggcctcggag ggcagccgca tcaccgtgaa gtagggctcc atctcgcggt
     1141 cgcgcccctc ctggggcagc cgcggcagca cccacaggtc ttccttgttg tagaagacct
     1201 ggggatcctg catgtggtag gtcgcgtaca tccgggcctg caccgtgaag agatcctccg
     1261 ggtaccggat gtgcggctgt agatccttcg gcatctcggc catcggccgg agcaggcccg
     1321 ggaacgcctt ggcgtaggtg cggatcagcg gatcctccgc gtccgcgatg aagtagctca
     1381 cggtgccgtg gtaggcgtcc acggtggcct tcaccgagtt gcggatgtag ttgaagccgc
     1441 gcaccggagt ggcgtacggg taccggtcgc tcgtgacgta gccgtcgacc atccacacca
     1501 gccggccgtc cgccccgatc accaggtagg ggtcgcggtc gaacttcagg aagggggccg
     1561 cctcctgcac ccgggtcgcg atgtcgcggt agatcatgat ccggctctgg ggcccgagcg
     1621 cgtccgagag caggatggtc agcaccccga agcgcgccgc gaaggccgcc ttccgcacga
     1681 tggagtcgac cgggatgccc ccccgcccct cgtacttggt gtagacgttc tggtccccgg
     1741 agggatagtc gagctcctgg gagcgggtgc ccacgaacac gtactcgttg ccgatctcgc
     1801 cgtagtagat ctcgggccgc gtgatcttgg gcaatcccgc gaccaccggc gggatgtcct
     1861 tgacgaagaa ctccgggaga ccctccgggc tgatgcggtt gaccggcccg gccacgagcc
     1921 cgtagccgtg ggtgaaggtc aggtgctcgt tgatccagcc ccggctcggc aggtcgcggt
     1981 aggagagctc gcgggccgag agggtgacct gtcggtactc cccgttgacc atgtaccgat
     2041 cgacgtccac gtcgcggaac ttgtagtagg tccggatctc ctggagcttg ccgtaggtgg
     2101 tcagcagcgg ccggtggtcc cagagcctca cgttcttgat cgtgaggttg ttccgctcga
     2161 gcgcggcctg gttcaggttc tcctccgccg cgaagtcctt ctccacgatc cggtcgagcg
     2221 cgtaggcctg ccgcgtcatc cggatgttgt gctggatgta cggccgctcg aagtccagct
     2281 cgttgggctt gacccggtag gtctggagca gcgcgggggc caccccgagg ccggcgatcc
     2341 acagcaccac cagcaccacg agcccggcca ccaggaagcg ccagcccggc cggaacatct
     2401 ggaacacgca cgcggccgcg cacagcaccg cgagcaccac cagccactgc agcaccggca
     2461 gcgaggcgtg cacgtcggtg taggaggcgc cgaacacgag cccgcggggc gagtagagca
     2521 ggtcgaagcg gtccagccag aagccaatcc cccggatgac gagcagcagc gcgccgaggc
     2581 cgagcaggtg ggtgcgcgcc cccgcggcca ggcgggggcc gcgcgcggtg agcaccaggc
     2641 tccgctggag cacgtacacc gccgcgacca gcacgagggt gcccgccacc agcgcggtgc
     2701 cccagccgtg gagcagccgc cagaacggga gcacgaagaa gtagaagccg aggtcgcgcc
     2761 cgaagagcgg atcgacctgg ccgaaggcgg tgccgttgag gtactcgatc acggtcggcc
     2821 aggagccggt ggcgcgggcc cccgcgaaga tcgagatcac cccgatgacc ggcagcagga
     2881 ggcggcgcac cagcggctcg aggatggcgc gcccgggtag cccgagctga tcctccagct
     2941 cccacagcac gtcgggcggc gcggtccggg ccgccaccga gagattgacg aagaggaaga
     3001 c
//
LOCUS       DATMCV010000197         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_674159, whole genome shotgun sequence.
ACCESSION   DATMCV010000197 DATMCV010000000
VERSION     DATMCV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674159"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1087
                     /locus_tag="VNU03_04560"
     CDS             50..1087
                     /locus_tag="VNU03_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP73757.1"
                     /translation="MRLRAAALVPLLILIACASGGTEGVAGPTHHAPGGGFRNLDPTF
                     TRPSGWTRWTFILRRSWQTFTSPRAFDAPRVANDGAALRAVPATASLTWIGHATLLVQ
                     VDGLTILTDPQWSDRAGPTSWIGPRRLSPPGLAFESLPHVDVVVISHDHFDHLDLPTV
                     RRLAATHDPLFLVPLGLKAWFQDNGMTHVEELDWWQEHEQRGVRFVCVPAQHFSQRSL
                     WDASRRLWASWVIAGRERRLYFGGDTGYFDGFKEAGRRLGPFDVAALAIGAYSPAEIM
                     KAVHTTPEEAVQAFVDLDARIMLGIHWGTFDLAEEPLDEPPVRMRADFERRRLDPARG
                     WIPKIGETRPW"
     gene            1101..1790
                     /locus_tag="VNU03_04565"
     CDS             1101..1790
                     /locus_tag="VNU03_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP73758.1"
                     /translation="MAHRVLIVEDEPDIRDLLVFHLEREGYQVVQSRSGPEALRLARA
                     TPPDLVLLDLMLPEMDGLEVCRRLRQDPATMALPIVMLTARGDEVDRVLGLELGADDY
                     VVKPFSPRELVARIRAVLRRARPPAGTTPLVIGGLAIDAAAHQVTVDGAAVSLTRKEF
                     DLLRALAEARGRVLSREYLLDHVWGYTAAGEIESRTVDVHVRRLRQKLGAEGQRIGTV
                     TGVGYRFEGDR"
     gene            1815..>2265
                     /locus_tag="VNU03_04570"
     CDS             1815..>2265
                     /locus_tag="VNU03_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73759.1"
                     /translation="MRALLRLVRRSIALKLTLTLVGFVAVSILAAGLYLNRALEAFAV
                     ESLDTRLAALAAVLDGDARAALVAGGPAAQDFAYRVARPTGSRVTLIAPDGRVVGESD
                     RSLAELSQLENHAGRPEVRAALEGRRGRDLRRSTTVAAPLLYMAAPVR"
BASE COUNT          278 a          837 c          838 g          312 t
ORIGIN      
        1 cggcctaggc tttacgcttt cgccgagacc gcggtagact gcccgcagag tgcgcctgcg
       61 agccgccgcg ctggtcccct tgctgatcct gatcgcctgc gcgagcgggg gcacggaggg
      121 cgtggccggg cctacccatc acgcgccggg cggcggcttc cggaacctcg accccacgtt
      181 cacccggccc tcgggctgga cccgctggac gttcatcctg cggcggagct ggcagacctt
      241 cacgtcgccc cgcgccttcg acgccccccg ggtggccaac gatggggccg cgctccgcgc
      301 ggtgccagcc accgcctcgc tcacctggat cgggcacgcg acgctgctgg tgcaggtcga
      361 cgggctcacc atcctcaccg atccccagtg gagcgaccgg gccggcccca cgtcgtggat
      421 cgggccccgc cgcctgagcc cgccggggct cgcgttcgag agcctgcctc acgtcgacgt
      481 ggtggtgatc tcccacgacc acttcgacca cctcgacctg cccaccgtcc ggcggctcgc
      541 cgcgacgcac gatccgctct tcctggtgcc gctcgggctc aaggcctggt tccaggacaa
      601 cggcatgacg cacgtggagg agctcgactg gtggcaggag cacgagcagc gcggggtgcg
      661 cttcgtctgc gtcccggcgc agcatttctc gcagcgctcg ctctgggacg cgagccggcg
      721 cctgtgggcc tcgtgggtca tcgcgggccg cgagcggcgg ctctacttcg gaggcgacac
      781 cggctacttc gacggcttca aggaggcggg ccggcgcctc ggcccgttcg acgtggccgc
      841 gctcgccatc ggcgcctaca gcccggccga gatcatgaag gcggtccaca ccacgcctga
      901 ggaagcggtc caggccttcg tcgatctcga cgcgcggatc atgctcggca tccactgggg
      961 caccttcgac ctggccgagg agccgctcga cgagcccccg gtgcgcatgc gggccgactt
     1021 cgagcggcgg cggctcgacc cggcccgggg atggatcccc aagatcgggg agacgcggcc
     1081 ctggtgagcg gcgcgaggcc atggcccatc gggtgctgat cgtggaggac gagcccgaca
     1141 tccgagatct cctcgtcttc cacctggagc gcgagggcta ccaggtggtg cagtcccgga
     1201 gcggccccga ggccctccgc ctggcgcggg cgacgccgcc cgacctggta ctgctggacc
     1261 tcatgctccc ggagatggac gggctcgagg tgtgccggcg cctgcgccag gatcccgcga
     1321 ccatggcgct cccgatcgtc atgctcaccg cgcggggcga cgaggtcgac cgcgtgctcg
     1381 gcctcgagct gggggccgac gactacgtgg tgaagccgtt ctcgccgcgc gagctggtgg
     1441 cccgcatccg cgcggtgctg cggcgcgccc ggccgccggc cgggaccacg ccgctcgtga
     1501 tcggcgggct cgccatcgac gcggccgccc accaggtgac ggtggacggc gccgcggtga
     1561 gcctcacccg gaaggagttc gacctccttc gcgcgctggc cgaggcgcgc ggtcgcgtgc
     1621 tgtcgcgcga gtatctcctc gatcacgtgt ggggctacac cgcggcggga gagatcgaat
     1681 cgcggacggt ggacgtccac gtgcgccggc tccggcagaa gctcggcgcg gaggggcagc
     1741 gcatcggcac cgtgaccggg gtgggctacc gcttcgaggg cgaccggtga ggagagaccg
     1801 gtgaggggac gcagatgcgc gcgctgctga ggctcgtccg gcgcagcatc gcgctcaagc
     1861 tcacgctgac cctggtgggc ttcgtggcgg tgagcatcct cgcggccggc ctctacctga
     1921 accgggccct cgaggccttc gcggtcgagt ccctggacac ccggctggcc gcgctggccg
     1981 cggtgctcga cggcgacgcc cgcgccgcgc tcgtggccgg gggaccggcg gcccaggact
     2041 tcgcgtaccg ggtggcgcgg cccaccggct cgcgggtgac cctgatcgcg cccgacggcc
     2101 gcgtggtggg cgagtcggac cggagcctgg ccgagctgag ccagctcgag aaccacgcgg
     2161 gccggccgga ggtgcgggcc gcgctcgagg gccgccgcgg tcgcgacctg cggcggagca
     2221 ccaccgtcgc ggcccccctc ctctacatgg cggcgccggt gcgcg
//
LOCUS       DATMCV010000198         2417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_944712, whole genome shotgun sequence.
ACCESSION   DATMCV010000198 DATMCV010000000
VERSION     DATMCV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944712"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VNU03_04575"
     CDS             <1..492
                     /locus_tag="VNU03_04575"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWP73760.1"
                     /translation="PALVVDGHWNLLMSNQGAQRLMARFVSPEDMAAVGGRPNAMRLF
                     YHPRGMRPYIVNWEATAAALIQWLHHDLARGVGDDETRRLLAELLAYPDVPQKWRSLD
                     LDVTPVPFLAVEMRKDDFHVKFFSTLASLGVPYDITLHELRVECFFPADGASEALLRG
                     LAP"
     gene            489..758
                     /locus_tag="VNU03_04580"
     CDS             489..758
                     /locus_tag="VNU03_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWP73761.1"
                     /translation="MKYVLLYESSPDVRAKAPLHFPAHRARMQDFHWRGTLLMSGPFG
                     DPEREGAMGIFTTREAAEEFAQGDPFVLHGVVARWHVREWNEVLA"
     gene            755..1804
                     /locus_tag="VNU03_04585"
     CDS             755..1804
                     /locus_tag="VNU03_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP73762.1"
                     /translation="MTTAPARPTQFGRIHPPDEAWLAKNPPEPILEPDLPIIDTHHHL
                     WDRGGHRYFLPDFLADAATGHNLVATVFLECHSMYRAGGPAEMRPVGEIEFVTGQAAM
                     SASGMYGSARVAAGIVGFADLTLGDRVEPVLEAQLRAGGGRFRGVRHSAAWDASDIIG
                     NSEAAGGPHLYRRAEFRAGLACLTRLGLSLDAWVFHPQLGEVAELARAHPEANIILGH
                     VGGVLGYGPYTGRQKEVFAAWKAAMTEVARCPNVSLKLGGMMMRLAAYDYRAEPKPPT
                     STELTALWGPYFETGIELFGPSRCLFESNFPVDKMGIGWAPLWNAFKRIVAGASRAEK
                     EAMFAGTARRVYRLD"
     gene            1804..>2417
                     /locus_tag="VNU03_04590"
     CDS             1804..>2417
                     /locus_tag="VNU03_04590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP73763.1"
                     /translation="MDPYREANRGLWDAWTRVNLRSEMYDVEAFAAGRGRDLDPIARA
                     GPGDVRGKSLLHLQCHFGMDTIRWARHGATVTGVDFSEEAITAARALAARMGVPATFV
                     QSDLYDLPSSLTGRFDVVFTSHGVLGWLPDLERWAQIVAHYLAPGGVFYIVEAHPILM
                     CFDDRLTEPDLRLLYPYFHGPEPIRYAHTGCYSTPDSPIESVEH"
BASE COUNT          337 a          894 c          825 g          361 t
ORIGIN      
        1 ccggccctgg tcgtcgacgg gcactggaac ctgctgatga gcaaccaggg cgcccagcgg
       61 ctgatggcgc gcttcgtctc ccccgaggac atggccgcgg tgggcggccg cccgaacgcg
      121 atgcggctct tctatcaccc gcgcggcatg cgcccctaca tcgtgaactg ggaggccacc
      181 gcggcggccc tcatccagtg gctgcaccac gacctggccc gcggcgtcgg cgacgatgag
      241 acgcgccgcc tgctcgccga gctgctcgcc taccccgacg tgccccagaa gtggcgcagc
      301 ctcgatctcg acgtgacccc ggtcccattc ctggccgtcg agatgcgcaa ggacgacttc
      361 cacgtgaagt tcttctccac cctcgcgagc ctcggcgtgc cgtacgacat cacgctgcac
      421 gagctgcggg tggagtgctt cttcccggcg gacggagcga gcgaggcgct gctgcggggg
      481 ctcgccccat gaagtacgtg ctcctctacg agtcgtcgcc cgacgtgcgc gcgaaggcgc
      541 cgctccactt ccccgctcac cgggcccgca tgcaggactt ccactggcgc ggcacgctcc
      601 tgatgtcggg tcccttcggc gatccggagc gggagggtgc gatgggcatc ttcaccaccc
      661 gcgaggctgc ggaggagttc gcgcagggcg acccgttcgt gctccacggc gtcgtcgcgc
      721 ggtggcacgt ccgcgagtgg aacgaggtcc tcgcatgacc accgcgcccg cccggcccac
      781 ccagttcggc cgcatccatc cgcccgacga ggcgtggctg gcgaagaacc cgcccgagcc
      841 gatcctcgag ccggatctgc cgatcatcga cacccaccat cacctgtggg atcgcggcgg
      901 ccatcgctac ttcttgcccg acttcctggc cgacgcggcc accgggcaca acctcgtcgc
      961 caccgtgttc ctcgagtgcc actcgatgta tcgcgcgggc gggcccgcgg agatgcggcc
     1021 ggtgggcgag atcgagttcg tcaccggcca ggccgcgatg agcgccagcg gcatgtacgg
     1081 gtcggcccgc gtggccgccg gcatcgtggg cttcgccgac ctgaccctgg gcgaccgcgt
     1141 cgagccggtg ctcgaagccc agctgcgcgc gggcggcggg cggttccgcg gagtgcgcca
     1201 ctcggcggcg tgggacgcga gcgacatcat cggcaacagc gaggccgcgg gcgggcccca
     1261 tctctaccgg cgcgccgagt tccgcgccgg cctcgcgtgc ctgacccggc tcgggctctc
     1321 gctcgacgcg tgggtgttcc acccgcagct cggcgaggtg gcggagctgg cccgcgctca
     1381 tccggaggcc aacatcatcc tgggccacgt gggcggggtg ctcggctacg gaccctacac
     1441 cggcaggcag aaggaggtct tcgcggcctg gaaggcggcg atgaccgaag tcgccaggtg
     1501 ccccaacgtg agcctgaagc tcggcgggat gatgatgcgc ctggccgcct acgactaccg
     1561 ggccgagccg aagccgccca cctcgaccga gctgaccgcg ctgtggggcc cgtacttcga
     1621 gaccgggatc gagctgttcg ggccgagccg gtgtctcttc gagagcaact tcccggtcga
     1681 caagatgggc atcggctggg ccccgctctg gaacgcgttc aagcgcatcg tcgcgggcgc
     1741 ctcccgggcc gagaaggagg cgatgttcgc gggcaccgcg cgccgggtgt accgcctgga
     1801 ctgatggacc cgtaccgcga ggccaatcgc gggctctggg acgcctggac gcgcgtcaat
     1861 cttcgctccg agatgtacga cgtcgaggcg ttcgcggccg ggcgcggccg agatctcgat
     1921 ccgatcgcgc gggccggacc gggcgacgtg cgcgggaaga gcctcctcca cctgcagtgc
     1981 cacttcggca tggacaccat ccgctgggcc cgccacggcg ccaccgtcac cggggtcgac
     2041 ttctcggagg aggccatcac cgcggcgcgg gcgctcgcgg cgcggatggg cgtgccggcc
     2101 accttcgtgc agagcgatct ctacgacctg ccgtcgagcc tcacgggccg cttcgacgtg
     2161 gtcttcacct cccacggcgt gctcggctgg ctcccggatc tcgagcgctg ggcccagatc
     2221 gtcgcgcact acctggcgcc cggcggcgtc ttctacatcg tcgaggcgca cccaatcctc
     2281 atgtgcttcg acgaccgcct gaccgagccg gatctgcgcc tgctctatcc atacttccac
     2341 ggccccgagc ccatccgcta cgcgcacacc ggctgctaca gcactcccga ctcgccgatc
     2401 gagagcgtcg agcactg
//
LOCUS       DATMCV010000199         4886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_10496, whole genome shotgun sequence.
ACCESSION   DATMCV010000199 DATMCV010000000
VERSION     DATMCV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10496"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VNU03_04595"
     CDS             <1..532
                     /locus_tag="VNU03_04595"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HWP73764.1"
                     /translation="DGVEPPPSRVPSLASRTLVAPDATGFPPIPGVRRVLEVNRISVF
                     GDWVEPRPDSSKTYRPLVSRVDGDGNEVAGIRLPAIAVPLGTHTGWNVYREPFPDGEL
                     CDRDGTFAAFARTRAERLAAGDPRASIEERYGSHARYVARVDAAVAELVRARLLLPED
                     AERMRAAARRSDPLAP"
     gene            536..1063
                     /locus_tag="VNU03_04600"
     CDS             536..1063
                     /locus_tag="VNU03_04600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP73765.1"
                     /translation="MGLELRDARRAPADREWLTNVYPLYLHDLSEFDDHYYTLDDRGV
                     WMPDHRASWLEEDGDHPLILVDAGRRAGFALVNQAPSPHMKPGMDFRMSEFFVLRNLR
                     GQGIGRRAVFALFDRFRGKWEISELPRNAAAISFWRRVIGEYGGGRHEETTDERGVTQ
                     VIDTTGSTSASSPPA"
     gene            complement(1035..2114)
                     /locus_tag="VNU03_04605"
     CDS             complement(1035..2114)
                     /locus_tag="VNU03_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP73766.1"
                     /translation="MRRVALVVALIAAAVAGDGFPAARPSPARAQMPASRPVDGFSAA
                     ERSGSAYKNVDPEYRRASYWARLHSLVIGGTWLFTERRVAALPAGTPDWATLRGGSLA
                     ATVTWIGHSTVLVQLDGVTFLTDPNWNDRSGPFSGFIGVRRYTPPGIALDSLPRIDFV
                     LISHDHYDHLDEPTVRRLARKFDPQFIVPKGIKAWLADRGITNAIELDWGESVVVKGL
                     TVVCTPAQHGSGRTLLDQGRRLWSSWAVLGTKHFYFGGDTGYYRHFQQIGDALGPFEL
                     AALPIGSYTPRELAKPVHMSPEDAVQAFLDLRAAHLLGVHWGTFALAREPYDEPPMRL
                     AAEIERRHLDPATAWVLKPGETRRW"
     gene            complement(2114..3346)
                     /locus_tag="VNU03_04610"
     CDS             complement(2114..3346)
                     /locus_tag="VNU03_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWP73767.1"
                     /translation="MLPVLAAFLLTGACGHVQPHLEIPDLRIAEPSFRATLVGYAGGS
                     VVGGNRVDVLLNGDQIFPAKLDLIRSARKSITYAQYVFEEGAPAADTAQALAERCRAG
                     VKVHVLVDAVGSLMMPSQYRDWLTEAGCELVLYRPLSPWSIDRVNNRNHRRILVVDGW
                     VGVTGGSGTSGKWSGNGKQEGQWRDTDIRLEGPVVAQLQGAFVDNWLEATGAALGGPD
                     YFPSPIEARGAVESQIVRSSPAGGSVAMYTMFLLAMSSARRSIYITNPYFVPDDKMIN
                     ALIQARGRGVRVVLLLPGATDHNLVRQASRSQLGRLLQAGIKIYEYRAALLHAKTMVI
                     DSIWATVGSTNLDRRSFELNEELNLVVYNGEIARRLEGVFEDDLKGSREITYEAWSNR
                     GVVSRFLEVLSVPFRDQM"
     gene            complement(3394..4092)
                     /locus_tag="VNU03_04615"
     CDS             complement(3394..4092)
                     /locus_tag="VNU03_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWP73768.1"
                     /translation="MRTRAIVGGALVAAVFLSGCAIETASTTPASGRTGSTQPAPAAK
                     APAASQKTVDPAQVERLKKAMIPLLKAMDKPLASNQVKVGILDDPSINAANAGNGEFY
                     VTTGLLEKASDDQLLAVMAHEVAHQDLGHVAKAQALGTGIGIGAVILDQIIPGAGRVV
                     PVAGALIQSKYSRSEEYQADRHGVELLQRVGRNKDVMINTLTWLMNTAGDSGGGFLST
                     HPATGERIEELRRL"
     gene            complement(4089..4334)
                     /locus_tag="VNU03_04620"
     CDS             complement(4089..4334)
                     /locus_tag="VNU03_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73769.1"
                     /translation="MSSRFLLGAIVGGIAVYVWGDEIRRFASTRGRTARLAAAETLQA
                     VQSTAEEMFDSAKDQVTSTLQAGQDAIRPARLTRDRR"
     gene            complement(4527..4775)
                     /locus_tag="VNU03_04625"
     CDS             complement(4527..4775)
                     /locus_tag="VNU03_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73770.1"
                     /translation="MPYAQVGRVVTAALLATAGAALLFGLFRRRPAPVPVVRCPIHGI
                     AYDTELEICPECAKTPTVTGAAGPETPHLETKTTGGVR"
     gene            complement(4775..>4886)
                     /locus_tag="VNU03_04630"
     CDS             complement(4775..>4886)
                     /locus_tag="VNU03_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HWP73771.1"
                     /translation="GALPTWPYSSGWGPYPSGGAGLILLVLIILLLTGRL"
BASE COUNT          697 a         1726 c         1726 g          737 t
ORIGIN      
        1 cgacggcgtc gagccgcccc cgagccgcgt gccctccctc gcgagccgga ccctggtggc
       61 tccggatgcg accgggttcc cacccatccc cggcgtgcgc cgcgtgctcg aggtcaaccg
      121 gatctccgtt ttcggcgact gggtcgagcc gcggcccgat tcctcgaaga cctaccggcc
      181 actggtgtca cgcgtggatg gagacggcaa cgaggtggcc ggcatccgcc tgcccgccat
      241 cgcggtgccg ctcggcaccc acaccggctg gaacgtctac cgggagccct ttccggacgg
      301 cgagctctgc gatcgcgacg gcaccttcgc ggccttcgcg cgcacccggg ccgagcggct
      361 ggccgcgggc gacccgcggg cctcgatcga ggagcgctac ggcagccacg cgcggtacgt
      421 ggcgcgcgtc gacgcggcgg tcgcggagct ggtgagggcc cggctgctgc tcccggagga
      481 cgcggagcgg atgcgggccg cggcccggcg ctccgatccg ctcgccccct gagcgatggg
      541 cctcgagctg cgcgacgcgc gtcgcgcgcc ggccgatcgg gagtggctga ccaacgtcta
      601 cccgctctac ctgcacgacc tctccgagtt cgacgaccac tactacacgc tcgacgatcg
      661 cggcgtctgg atgcccgatc accgggcgag ctggctcgag gaggacggcg atcacccgct
      721 gatcctcgtc gacgcgggcc gtcgggcggg cttcgcgctc gtcaaccagg ccccctctcc
      781 ccacatgaag ccgggcatgg acttccggat gtccgagttc ttcgtgctcc gcaatctccg
      841 ggggcagggg atcgggcgcc gcgccgtgtt cgccctcttc gaccggttcc ggggcaagtg
      901 ggagatctcc gagctgcccc gcaacgcggc cgcgatcagc ttctggcgtc gcgtgatcgg
      961 agagtacggg ggcggccgcc acgaggagac gaccgacgag cggggcgtga cgcaggtcat
     1021 cgatacgacc gggtctacca gcgcctcgtc tcccccggct tgaggaccca ggccgtcgcc
     1081 ggatcgaggt gccgccgctc gatctcggcg gcgaggcgca tggggggctc gtcgtagggc
     1141 tcgcgggcga gcgcgaaggt gccccagtgc acgccgagga gatgcgcggc gcgcaggtcg
     1201 aggaaggcct gcaccgcgtc ctcgggtgac atgtgcaccg gcttcgcgag ctcccgcggg
     1261 gtgtaggagc cgatcggcag cgcggcgagt tcgaacgggc cgagggcgtc gccgatctgc
     1321 tggaagtggc ggtagtagcc ggtgtcgccg ccgaagtaga agtgcttcgt ccccagcacc
     1381 gcccaggagg accagagccg gcggccctgg tcgagcaggg tgcgcccgct gccgtgctgg
     1441 gccggcgtgc agaccaccgt gaggcccttc accaccaccg actcgcccca gtcgagctcg
     1501 atcgcgttgg tgatcccccg gtccgcgagc cacgccttga tccccttcgg cacgatgaac
     1561 tgcggatcga acttgcgggc gaggcgccgg acggtcggct cgtcgaggtg atcgtagtgg
     1621 tcgtgggaga tcaggacgaa gtcgatccgc ggcagggagt cgagcgcgat gccgggcggc
     1681 gtgtagcggc gcaccccgat gaagccgctg aacgggccgc tgcgatcgtt ccagttgggg
     1741 tcggtgagga aggtcacccc gtcgagctgc accagcaccg tcgagtggcc gatccaggtg
     1801 acggtcgcgg cgagggagcc tccgcgcagg gtggcccagt ccggcgtgcc cgcggggagg
     1861 gcggcgaccc gccgctcggt gaagagccac gtgcccccga tcaccagcga gtgtaggcgg
     1921 gcccagtagg aggcgcggcg atactccggg tcgacgttct tgtacgcgct cccggagcgc
     1981 tcggcggcgc tgaacccgtc caccggccgc gaggcgggca tctgcgcgcg cgcgggggag
     2041 ggcctcgcgg ccgggaagcc gtctcccgcc accgcggccg cgatgagcgc gacgacgagg
     2101 gcgacgcgac gcatcacatc tgatcgcgga agggcaccga caggacctcg aggaaccggc
     2161 tcaccacgcc gcggttgctc cacgcctcgt aggtgatctc acgcgatccc ttcaagtcgt
     2221 cctcgaacac gccctcgagc cggcgggcga tctctccgtt gtagaccacc aggttcagct
     2281 cctcgttcag ctcgaaggag cgccggtcga ggttggtgct gcccaccgtc gcccagatcg
     2341 agtcgatcac catcgtcttg gcgtggagca gcgcggcccg gtactcgtag atcttgatcc
     2401 ccgcctggag gagccggccc agctgggagc ggctcgcctg gcggacgagg ttgtggtcgg
     2461 tggcgccggg cagcaggagg accacgcgca cgccacgccc gcgggcctgg atcagggcgt
     2521 tgatcatctt gtcgtcgggc acgaagtagg ggttggtgat gtagatcgag cgcctcgccg
     2581 aggacatcgc gagcaggaac atggtgtaca tcgccacgct gccccccgcc ggggagctgc
     2641 gcacgatctg cgactccacc gcgcccctgg cctcgatcgg cgacgggaag tagtcgggtc
     2701 cgccgagggc ggctccggtc gcctcgagcc agttgtcgac gaacgcgccc tgcagctggg
     2761 cgaccaccgg gccctccagc cggatgtcgg tgtcccgcca ctgtccctcc tgcttgccgt
     2821 tgccgctcca cttcccgctc gttccggatc cgccggtgac tccgacccac ccgtccacca
     2881 cgaggatgcg gcggtggttg cggttgttga cgcggtcgat gctccacgga gagagcggcc
     2941 gatagaggac cagctcgcac cccgcctccg tcagccagtc gcggtactgc gacggcatca
     3001 tgagggatcc gaccgcgtcg accagcacgt ggaccttgac ccccgcgcga cagcgctccg
     3061 cgagggcctg cgcggtgtcg gcggcgggcg cgccttcctc gaagacgtac tgggcgtagg
     3121 tgatgctctt ccgcgcggag cggatgaggt cgagcttggc cgggaagatc tggtccccgt
     3181 tcagcaggac gtcgacgcgg ttgccgccga ccaccgagcc gcccgcgtac ccgaccaggg
     3241 tggcccggaa cgacggctcc gcgatccgga ggtcggggat ctccagatga ggctggacgt
     3301 gcccgcaggc gcccgtcagg agaaaagccg ccagcaccgg aagcactgga agtatcacgc
     3361 gggccccggt tgggccgcgc cgcctgaccg ggctcacagc ctccgcagct cttcgatccg
     3421 ctcccccgtc gcagggtggg tgctgaggaa gccgccgccg ctgtcccccg ccgtgttcat
     3481 cagccaggtg agcgtgttga tcatcacgtc cttgttgcgg ccgacgcgct ggagcagctc
     3541 gaccccgtga cgatccgcct ggtactcctc gctgcggctg tacttgctct ggatcagcgc
     3601 cccggcgacc ggtaccaccc ggccggctcc cgggatgatc tggtccagga tgaccgcgcc
     3661 gatcccgatg ccggtgccga gcgcctgagc cttggccacg tggcccaggt cctggtgggc
     3721 gacctcgtgg gccatcacgg ccaggagctg atcgtcgctc gccttctcca ggagcccggt
     3781 cgtgacgtag aactcaccgt tgcccgcatt ggccgcattg atggacggat cgtccaggat
     3841 cccgaccttg acctggttcg aggccagcgg cttgtccatg gccttgagca ggggaatcat
     3901 cgccttcttc aagcgctcga cctgggccgg atcgaccgtc ttctgcgaag cggcgggcgc
     3961 cttcgcggcg ggtgccggct gggtggatcc ggtccggccg gacgcgggcg tcgtcgaggc
     4021 ggtctcgatc gcgcagccgc tcaggaagac ggccgcgacg agggcgcctc caacgatcgc
     4081 ccgcgtcctc atcgccgatc ccgggtgaga cgggccgggc ggatggcgtc ctgacccgcc
     4141 tgcagggtcg aggtcacttg atccttggcc gaatcgaaca tctcctccgc ggtcgactgg
     4201 accgcctgca gcgtctcggc cgccgcgagc cgcgcggtgc ggccccgggt gctcgcgaag
     4261 cgacggatct cgtcgcccca cacgtatacc gcgatgcctc cgacgatcgc tcccagcagg
     4321 aatcgacttg acatcactcg ctcctttctc tctccgcgcc tatcacgcct acgcggcttt
     4381 cggaattgac cactcgaccc ccgcgctccg gtggagctgg tggccgcagg gctcgagctg
     4441 gatcaccagc gcgtcgccgc gctcgtgcca gtcctcgacc gcccggaacg ctccacagga
     4501 aaggcaatac agcgtcggtg tccctctcat cgcacacctc ccgtcgtctt ggtttccagg
     4561 tgaggggtct ccgggccggc ggcaccggtc acggttggcg tcttggcgca ctccgggcat
     4621 atctctagtt cggtgtcgta ggcgatgccg tggatcgggc atctcacgac cgggacgggc
     4681 gcgggacggc ggcggaagag cccgaacaga agggccgcgc ccgcggtggc gagcagcgcc
     4741 gccgtcacca cccgtccgac ctgcgcgtag ggcatcagag ccgtccggtg aggagcagga
     4801 tgatgagaac cagcagaatg aggcccgcgc caccgcttgg atacggcccc cagcccgagc
     4861 tgtacggcca ggtcggcaag gccccc
//
LOCUS       DATMCV010000200         6163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_674469, whole genome shotgun sequence.
ACCESSION   DATMCV010000200 DATMCV010000000
VERSION     DATMCV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674469"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VNU03_04635"
     CDS             complement(<1..936)
                     /locus_tag="VNU03_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73772.1"
                     /translation="MSAPLLEAQGLRKHFPLGGGILQRTRTWVRAVDGVSFSVAPGET
                     LGIVGESGCGKSTLARLLLRLIEPDAGRVTFDGEDVRAASASRMKRLRRDMQLVFQDP
                     YASLNPRLTIEDTVGFSLLVHGTPRAEARERARALLGRVGLDPRLYGRRYAHELSGGQ
                     RQRVNIARALVLDPRLLVLDEPVSALDKSVQAQVLNLLQELKAERALTYVLISHDLNV
                     IEYVSDRVLVMYLGEVVETGSAEALAQAPKHPYTQALFASAPSMDPDRRMDRLPITGD
                     PPNPIDPPSGCRFRTRCPFAMPRCAEAPPPLVPVSA"
     gene            complement(933..1820)
                     /locus_tag="VNU03_04640"
     CDS             complement(933..1820)
                     /locus_tag="VNU03_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73773.1"
                     /translation="MATTVALGEVPVVMAAPMSYWRTVSWRLARDRATLLAGGVLAAI
                     VLSALLAPWLAPYDPTAGSIRLRLAPVGAPGHVLGTDEQGRDMLSRLLWGGRMTLVAG
                     LTPVLVAFVLGSGLGVLAGYLGGIANTLIMRTMDVFYAFPSVLLAIAISGALGPGLAN
                     TVIALSIVFVPRVVRVAESATVQVKSLEYIAAARASGAGVRAIVRHHLLANVLGPILV
                     FSSTQVSLSIILAAGLSFLGLGVTPPAAEWGLMLNTLRQSIYVNALVPALPGLAIFVT
                     SMAFNLLSDGLRDAMEIRG"
     gene            complement(1820..2758)
                     /locus_tag="VNU03_04645"
     CDS             complement(1820..2758)
                     /locus_tag="VNU03_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73774.1"
                     /translation="MWVYVGRRLLLAIPTLLGASVIVFAMVHLAPGDPIAAVMPAESS
                     QAEIEATKRAYGFDRPLPVQYLVWLGRVAQGDLGKSIATRRPVVLDVRDAFANSLVLA
                     LSSSLVAFGLGGALGLLAAFRQGRVVDKLASAIAVVGVSVPHYWAGIILIIVFSVQLN
                     WLPAMGSANDPGLVEYLRHIALPTVALALIPLGVITRVVRSAALDILNQEFVMALRAK
                     GLAARRVFTHVVKNAAPQILTVLGLQFGFQLGGSVLVEAVFSWPGTGYLLNLAIFQRD
                     IPVLQGVILVLATFFVLLNLAVDLAQTLIDPRIARH"
     gene            complement(2768..4363)
                     /locus_tag="VNU03_04650"
     CDS             complement(2768..4363)
                     /locus_tag="VNU03_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73775.1"
                     /translation="MRRIVILLLALALPLSSAGPASAQGTLRIGMTASDIPYTGGQSD
                     NGFEGFRFVGYQIYEPLIQWDLSRGDRLAQLVPGLAESWEVRKDAPTRWVFKLRKGVT
                     FHDGSPFNADAVIFSFESIKKKDAPHFDPYGSGQVGFRVASLVAVTKVDDHTVEFETS
                     KPTSFVPYQICYMVIVSPTQWQKFKDWRKFAEQPSGTGPFRVTKFVPRERLELEAYKG
                     YWDAKRRPKIDKLVLLPMPEPTTRLAALRSGQVDWIEVPPPDAIPQLKGAGFTIALNS
                     YPHNWTHTLRLDKEPWSNKLVRKAANYAIDRVGICKNLLNDTCIPATGVVYKGHPWFG
                     KPKETYDYNPAKAKDLLRQAGFDASKHPAKAVHLISTSGSGQMLPLAMNELIQKNLKD
                     VGIDVDLQPVEWNTLLTRWRAGFKTPENEGLNAWNISWNFLEPWSGFGRFFHSKAVVP
                     VAVNTMPYINPEADKLIDEAERTFDVGKQNEILARLHEVVVDDAPWIFVVHDQNPRAL
                     SPKVKGFVQPQSWFVDLTSVTVR"
     gene            complement(4374..5333)
                     /locus_tag="VNU03_04655"
     CDS             complement(4374..5333)
                     /locus_tag="VNU03_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73776.1"
                     /translation="MALLTVRDLSVAFETRDGTVRAVSDVSFSLEAGRVLAILGESGS
                     GKSVTLRAVLGLHQGPHARVSGVVSVKGQDVNRLDERARESLRGRVVSMVFQEPMTAL
                     DPVYTIGTQIAETLAQHRGLGRAAALGRARELLDLVQVPSPARCLASYPHELSGGMRQ
                     RAMIAIALACEPALLLADEPTTALDVTVQAQILWLLRDLQKRLDLAVVFVTHDLGVAA
                     EIADDAAVMYGGRIVEQAPVRELFRRPAHPYTAGLMQATVRRGQKGRPLVPIAGAPPN
                     LAALPPGCAFAPRCPVAREDCATTAPSFHPVAPGHTARCHLVA"
     gene            5437..6027
                     /locus_tag="VNU03_04660"
     CDS             5437..6027
                     /locus_tag="VNU03_04660"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HWP73777.1"
                     /translation="MPTRIRFLLAPMLLTLAGCVRPPVTPGLVCVPSSQPPPYAAAGS
                     WYCQEVSVSVREPGPQIVQHRTGQFADLIVSAVGRCPERAGQFAPSVTVKNIGPVPAG
                     AFNVAVDVGVFDSGGGAAVSTAAIVRVPGLAAGADTTVAFDRMQGVLLRAGDRLTISA
                     VADPQQVPGSPLNVFGEILELDETNNSAVQNCTVPN"
BASE COUNT          957 a         2139 c         2227 g          840 t
ORIGIN      
        1 ggcgctcacc ggcacgagcg gcggaggcgc ctccgcgcag cggggcatcg cgaacgggca
       61 gcgggtgcgg aagcgacaac cggagggtgg gtcgatcgga ttcggcggat cgcccgtgat
      121 gggcagacgg tccatgcgcc ggtcgggatc catgctcggg gcggaggcga agagcgcctg
      181 ggtgtaggga tgcttcggcg cctgggcgag tgcctccgcg ctcccggtct ccacgacctc
      241 gccgaggtac atgacgagca cccggtcgct cacgtactcg atgacgttca gatcgtgcga
      301 gatgagcacg taggtgagcg cgcgctcggc cttgagctcc tgcagcaggt tcagcacctg
      361 cgcctgcacc gacttgtcga gcgcggacac cggctcgtcc agcaccagca gccgcggatc
      421 gagcacgagc gcgcgcgcga tgttcacccg ctggcgctgg cctccgctca gctcgtgggc
      481 gtagcgccgg ccgtagagcc tcggatcgag gccgacgcgg cccaggagcg cgcgcgcccg
      541 ctcgcgcgcc tccgcccgcg gggtgccgtg cacgagcagg ctgaacccca ccgtgtcctc
      601 gatggtcagg cgcgggttca gcgacgcgta ggggtcctgg aagacgagct gcatgtctcg
      661 gcgcagccgc ttcatgcgcg aggcggaggc cgcccgcacg tcctcgccgt cgaacgtcac
      721 ccggcccgcg tccggctcga tcaatcggag gagcagccgc gccagcgtcg acttgccgca
      781 gcccgactcc cccacgatgc cgagggtctc gcccggcgcc acgctgaacg agacgccgtc
      841 caccgcccgc acccaggtcc gggtacgctg caggatgccc ccccccagcg ggaagtgctt
      901 gcgcagaccc tgcgcctcca gcaggggcgc gctcagccgc gaatctccat cgcgtcgcgc
      961 aggccgtcgg acagcaggtt gaacgccatc gacgtgacga agatggcgag gccgggcagc
     1021 gcgggcacca gcgcgttcac gtagatcgat tggcggagcg tgttcagcat cagcccccac
     1081 tccgcggcgg ggggcgtcac cccgaggccg aggaacgaca ggccggcggc gaggatgatc
     1141 gagagcgaca cctgggtgct cgagaacacc aggatggggc ccagcacgtt ggccaggagg
     1201 tggtgtcgca cgatggcccg cacgcccgcg ccgctggccc gcgccgccgc gatgtactcc
     1261 aggctcttca cctgcacggt ggcggactcc gcgacccgca ccacgcgcgg cacgaagacg
     1321 atcgacagtg cgatgacggt gttcgcgagc ccgggcccga gggcgccgga gatcgcgatc
     1381 gcgagcagga ccgaggggaa cgcgtagaag acgtccatcg tgcgcatgat cagggtgttg
     1441 gcgatcccgc cgaggtagcc ggccagcacc cccagcccgg acccgagcac gaaggccacc
     1501 agcaccgggg tgagcccggc caccagggtc atgcgaccgc cccacagcag gcggctcagc
     1561 atgtcgcgcc cctgctcgtc ggtccccagc acgtggccgg gcgcgccgac cggcgcgagc
     1621 cgcaggcgga tcgaccccgc ggtcgggtcg tacggggcga gccacggcgc cagcagcgcg
     1681 gacagcacga tggccgccag cacacccccg gccaggaggg tggcgcggtc gcgcgcgagc
     1741 cgccagctca ccgtccgcca gtaggacatc ggcgcggcca tgaccaccgg gacctcgccc
     1801 agcgcgacgg tggtggccat cagtggcgcg cgatccgcgg gtcgatcagg gtctgggcca
     1861 gatcgaccgc gagattcagg agcacgaaga acgtggcgag caccaggatc acaccctgca
     1921 gcaccgggat gtcgcgctgg aagatggcca ggttgaggag gtagccggtt cccggccacg
     1981 agaagaccgc ctccaccagc accgagccgc cgagctggaa gccgaactgc agcccgagca
     2041 cggtgaggat ctgcggggcc gcgttcttca ccacgtgcgt gaagacccgc cgggcggcca
     2101 gccccttggc ccgcagggcc atcacgaact cctggttcag gatgtcgagc gcggccgagc
     2161 gcaccacgcg ggtgatgacc ccgaggggga tgagggccag cgccaccgtg ggcagcgcga
     2221 tgtgccgcag gtactccacg aggccgggat cgttcgcgga ccccatggcg ggcagccagt
     2281 tgagctgcac cgagaagacg atgatcagga tgatcccggc ccagtagtgc ggcaccgaga
     2341 ccccgaccac cgcgatcgcg ctcgccagct tgtcgaccac ccgtccctgc cggaacgcgg
     2401 cgagcaaccc gagcgccccg cccagcccga acgcgaccag cgaggacgag agcgccagca
     2461 ccaggctgtt ggcgaaggcg tcgcgcacgt cgaggaccac cggccggcgg gtggcgatcg
     2521 acttgccgag atcaccctgg gccacccgcc cgagccagac gaggtactgc accggcaggg
     2581 gccggtcgaa gccgtacgcg cgcttggtgg cctcgatctc ggcctggctc gactcggccg
     2641 gcatcaccgc cgcgatcgga tcgcccgggg ccaggtgcac catcgcgaac acgatgacgc
     2701 tggccccgag cagggtcggg atcgcgagca ggagacgccg gccgacgtag acccacacga
     2761 ggcggcgtta ccgcacggtc accgaggtca gatccacgaa ccagctctgg ggctggacga
     2821 agcccttcac cttcggggag agcgcgcgcg ggttctggtc gtgcaccacg aagatccacg
     2881 gcgcgtcgtc gaccaccacc tcgtgcaggc gggcgaggat ctcgttctgc ttgcccacgt
     2941 cgaaggtgcg ctccgcctcg tcgatgagct tgtccgcttc cgggttgata tagggcatcg
     3001 tgttgaccgc gaccggcacc accgccttgc tgtggaagaa ccggccgaac ccgctccacg
     3061 gctcgaggaa gttccacgag atgttccagg cgttgaggcc ctcgttctcc ggggtcttga
     3121 agccggcccg ccagcgggtc agcagggtgt tccactccac cggctgcagg tcgacgtcga
     3181 tcccgacgtc cttcaggttc ttctggatca gctcgttcat cgcgagcggc agcatctggc
     3241 ccgagccgga ggtcgagatc aggtgcaccg ccttcgcggg atgcttggag gcgtcgaacc
     3301 cggcctggcg cagcaggtcc ttggccttcg ccgggttgta gtcgtaggtc tccttgggct
     3361 tgccgaacca ggggtggccc ttgtagacca cgccggtggc ggggatgcag gtgtcgttca
     3421 gcaggttctt gcagatgccg acgcgatcga tcgcgtagtt ggccgccttc cgcaccagct
     3481 tgttgctcca cggctccttg tcgagccgca gggtgtgggt ccagttgtgc gggtagctgt
     3541 tgagcgcgat ggtgaagccg gcccccttga gctgggggat cgcgtcgggc ggcggcacct
     3601 cgatccagtc gacctggcca ctgcgcagcg cggccagccg ggtggtcggc tccggcatcg
     3661 gcagcagcac cagcttgtcg atcttggggc ggcgcttggc gtcccagtac cccttgtagg
     3721 cctccagctc gagccgctcc cgcggcacga acttggtcac ccggaaggga ccggtgccgg
     3781 agggctgctc ggcgaacttg cgccagtcct tgaacttctg ccactgggtg ggcgacacga
     3841 tgaccatgta gcagatctgg tagggcacga agctggtcgg cttgctggtc tcgaactcga
     3901 cggtatgatc gtcgaccttg gtgaccgcca ccagggaggc gacccggaag ccgacctgcc
     3961 cggagccgta gggatcgaag tgcggcgcgt ccttcttctt gatcgactcg aaggagaaga
     4021 tcaccgcgtc cgcgttgaac gggctgccgt catggaaggt gacgcccttg cgcagcttga
     4081 acacccaccg ggtcggcgcg tccttgcgca cctcccagga ctcggcgaga ccgggcacca
     4141 gctgcgcgag gcggtcgccg cgactgagat cccactggat cagcggctcg tagatctggt
     4201 agccgacgaa gcggaacccc tcgaacccgt tgtcgctctg gccgccggta taggggatgt
     4261 cgctggcggt catgccgatt cgcagcgtgc cctgggcgga ggccgggccg gccgacgaga
     4321 gcggcagcgc gagggcgaga agcaggatga cgatgcgtct catgacgatc ctcctaggcg
     4381 acgaggtgac agcgggcggt gtggccgggg gccacggggt ggaacgacgg cgcggtggtc
     4441 gcacagtcct cgcgggcaac cgggcaccgg ggcgcgaacg cgcagccggg cggcagcgcg
     4501 gccaggttcg gcggcgcgcc cgcgatcggg acgagggggc ggcccttctg accgcggcgc
     4561 acggtggcct gcatgagccc ggcggtgtac gggtgcgcgg ggcggcggaa cagctcgcgc
     4621 accggggcct gctcgacgat gcgcccgccg tacatcaccg ccgcgtcgtc cgcgatctcg
     4681 gccgcgaccc ccagatcgtg ggtgacgaag acgaccgcga ggtcgaggcg cttctgcaga
     4741 tcgcgcagga gccagaggat ctgggcctgg accgtgacgt cgagggcggt ggtcggctcg
     4801 tcggcgagga gcagggccgg ctcgcacgcg agcgcgatcg cgatcatcgc ccgctggcgc
     4861 atgccgccgg agagctcgtg cgggtagctc gccaggcagc gcgcgggcga cggcacctgc
     4921 accaggtcga gcagctcgcg ggcccgcccg agcgcggccg cgcggccgag gccgcgatgc
     4981 tgggcgaggg tctcggcgat ctgcgtgccg atggtgtaca ccgggtcgag cgcggtcatc
     5041 ggctcctgga acaccatcga gaccacgcgg ccgcgcaggc tctcgcgcgc gcgctcgtcg
     5101 agccggttca cgtcctggcc cttcacggag accacgccgc tcacccgcgc gtgcggaccc
     5161 tgatgcagtc cgaggacggc ccggagggtg acgctcttgc ccgagccgga ctcaccgagg
     5221 atggcgagga cccggcccgc ctcgagcgag aaggagacgt cgctcaccgc gcgcacggtt
     5281 ccgtcgcgcg tctcgaatgc cacgctgagg tcgcggacgg tcaggagggc cacgccagat
     5341 gtccccaagt gcgggcgatc atagcgggat tgccgatcga gcgccaccgc ggaacgtggt
     5401 acgctcgcga tcgcgttcag agggaggagc gctcccatgc cgacgaggat ccggtttctt
     5461 ctcgccccga tgctgctgac cctcgccggt tgcgtccgcc cgccggtgac ccccggcctg
     5521 gtctgcgtgc cgtcgagcca gccgccgccc tacgcggcgg ccgggagctg gtactgccag
     5581 gaggtatcgg tcagcgtgcg cgagcccggc ccccagatcg tccagcaccg gaccggccag
     5641 ttcgcggacc tcatcgtgag cgcggtcggt cgctgcccgg agcgcgcggg ccagttcgcc
     5701 cccagcgtca cggtcaagaa catcgggccg gtgcccgcgg gagcgttcaa cgtggccgtc
     5761 gacgtcgggg ttttcgacag cggcggcgga gccgcggtct cgaccgcggc gatcgtgcgc
     5821 gtcccgggcc tcgccgcggg ggcggacacc accgtcgcct tcgaccgcat gcagggggtg
     5881 ttgctgcggg ccggggatcg gctcaccatc tccgcggtcg ccgatccgca gcaggtgccc
     5941 ggcagccccc tcaacgtgtt cggcgagatc ctcgagctgg acgagaccaa caacagcgcg
     6001 gtccagaact gcacggtacc gaactagccc ggcgccgcct caagggaact cggcccgcag
     6061 cgcgggcatc acgtcccgcg cgaatcgctc catgctgcgc atcgtgaggt cgtgcggcat
     6121 gctccccacc tggaagatgc cctggatgat gccgcagccg gcc
//
LOCUS       DATMCV010000201         4070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_745654, whole genome shotgun sequence.
ACCESSION   DATMCV010000201 DATMCV010000000
VERSION     DATMCV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745654"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VNU03_04665"
     CDS             complement(<1..696)
                     /locus_tag="VNU03_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73778.1"
                     /translation="MTPAIALIVAVLLLLGAPPPAGAQPAGKVWRIGYLSLTSDSVSY
                     RPWLAALRDGLRDLGYVDGQNIIIEPRYAAGQVERLSPLAAELVRLKVDVLVTAPAGT
                     ALAARSASRTIPIVFIGEPDPVGTLVASLAHPGGNVTGLSDAHADLVPKRLELLKQVV
                     PSVTRVGILWSPANPGTAPQVRIAEGAAPALGLTVLPVAVKGPRRDDLDRAFATIERE
                     RLGSLFVIGDSTLG"
     gene            complement(698..1591)
                     /locus_tag="VNU03_04670"
     CDS             complement(698..1591)
                     /locus_tag="VNU03_04670"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HWP73779.1"
                     /translation="MQGYSPPPPPVSPIKGLIDFHTHAAPDIFGRAIDDDELAALAAS
                     RQMEAIVFKNHVTHTADRAWLVRKHVTGIKVFGGITLNRAVGGLNPQAVEWMWRMQGG
                     YGRVVWFPTFDADNHVRKSGTAPSGLRVVDERGQVLPEARAILKICAAQRLVVQTGHA
                     SAEEALALIEAAREEGCDRVVVTHAQFDVVDMSLAQMKKAAALGGKMELCALLMLTGP
                     DSPLEWMRHAPRIPLADTAARIKAVGAQHFVLGTDLGQTGNPTPADGLQMFVTGLLAQ
                     GITREQIETMGREVPGALLMS"
     gene            1914..3071
                     /locus_tag="VNU03_04675"
     CDS             1914..3071
                     /locus_tag="VNU03_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWP73780.1"
                     /translation="MPLPDRPSLEFLKKLAKDRLPEMRRTAPSACLADAQLAVAREHG
                     FPSWRALRAELHRLAAAAPDPPDLVVQLFAAIRSGEGPVVDRLLAAHTGLANARDEEG
                     STPLSAAVDAHRAALIPILLRRGGDPHLVYAHSAHTPLSWAVVIEAYDCARALMEAGV
                     EPDLFCAAGLGEVDRVRTFFSPEGRLRPRASITGSSRYAPDGTRLPCPPATDREVVSD
                     ALYMASRAGHEGTVRELLTHGPDLAFPAFAGATALHWAHFAGAPRVVALLLAAGADPT
                     LRDPVLGCTPEAFGICTPASWAWGSKVRQRLAENPGLVHAESTRGGPLHEAAWAGALE
                     AVKLLLEAGADPARRNAEGKTALDLARERPDQPWCVKVVEWLTSSAPEPRR"
     gene            3074..3394
                     /locus_tag="VNU03_04680"
     CDS             3074..3394
                     /locus_tag="VNU03_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HWP73781.1"
                     /translation="MFFELRQYHVRPGQQEKWVKCMEEEIIPFQVKMGMVVLGSFVGE
                     EDASVYVWIRRFESEPERKRLYDAVYQSDYWKNEISPRVPQMIDREQIKVTRIVATPH
                     SVIQ"
     gene            3405..>4070
                     /locus_tag="VNU03_04685"
     CDS             3405..>4070
                     /locus_tag="VNU03_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73782.1"
                     /translation="MSQAPLEAVRALGPLIRSSAAETDAQRELARPLFEAMADAGLFQ
                     LGLPRAVGGAELDLPSYIQVIEEIGKGDASTGWVTNQVAIFATYAARMPREAARAIWI
                     DTPRSVVANTPMASAQAVVVPGGYRVTGRQGFSTGCRHASWLAAHATVMEQGRPRLDE
                     GQPELRYCFVPRAQAELLDTWQVRGMRGTGTHHFAVDDVFVPEERTVKSVTAPLIETG
                     PLYQ"
BASE COUNT          574 a         1435 c         1504 g          557 t
ORIGIN      
        1 tccgagggtc gagtcaccga tgacgaagag actcccgaga cgctctctct cgatcgtggc
       61 gaacgcccgg tccaggtcgt cacgtcgcgg gcccttgacc gcgaccggga gaacggtcag
      121 gcccagggcg ggagcggcgc cctcggcgat cctcacctgc ggcgcggtgc ccggattggc
      181 gggactccac aggatgccga cccgagtgac cgacgggacg acctgtttga gcagctcgag
      241 ccgcttcggg acgaggtcgg catgggcatc cgacagcccc gtgacgttcc caccgggatg
      301 ggcgaggctc gccacgagcg ttccgaccgg atcgggctcg ccgatgaaca cgatggggat
      361 cgtgcggctg gcgctcctgg ccgccagcgc ggtgccggcc ggcgcggtga cgaggacgtc
      421 gaccttcagc cggacgagct cggccgcgag cggggagagt cgctcgacct gaccggccgc
      481 gtagcgcggc tcgatgatga tgttctggcc gtccacgtat cccagatccc ggagcccgtc
      541 ccgaagggcg gcgagccagg gccggtacga caccgaatcg gaagtgagcg agagatagcc
      601 gatccgccag acctttcccg ccggctgcgc cccggcggga ggcggcgccc cgaggagaag
      661 cagaaccgcg acgatgaggg cgatggccgg cgtcatgtca gctcatcaag agcgcgccgg
      721 ggacctcgcg gcccatggtc tcgatctgct cgcgggtgat gccctgggcg agcaggccgg
      781 tgacgaacat ctggaggccg tcggcgggcg tggggttgcc ggtctggccg aggtcggtgc
      841 cgaggacgaa gtgctgcgcg cccaccgcct tgatgcgcgc ggcggtgtcg gcgagcggga
      901 tgcgcggggc gtggcgcatc cactcgagcg gggagtcggg gccggtcagc atgagcagcg
      961 cgcagagctc catcttgccg cccagcgcgg ccgccttctt catctgcgcg aggctcatgt
     1021 cgaccacgtc gaactgggcg tgggtcacca ccacccggtc gcagccctcc tcgcgcgcgg
     1081 cctcgatcag ggccagcgct tcctcggccg aggcgtggcc ggtctgcacc accaggcgct
     1141 gcgcggcaca gatcttgagg atcgcgcgcg cctcgggcag gacctgcccg cgctcgtcga
     1201 cgacgcggag gcccgacggc gcggtgccgc tcttgcggac gtggttgtcg gcgtcgaagg
     1261 tggggaacca caccacccgg ccgtatccgc cctgcatgcg ccacatccac tcgaccgcct
     1321 gcgggttgag cccgcccacc gcccggttga gggtgatgcc gccgaagacc ttgatgccgg
     1381 tgacgtgctt gcgtacgagc caggcccggt cggcggtgtg ggtcacgtga ttcttgaaca
     1441 cgatcgcttc catctgtcgc gaggcggcca gggcggccag ctcgtcatcg tcgatcgccc
     1501 ggccgaagat gtcgggcgcc gcgtgggtgt ggaagtcgat caggcccttg atcgggctga
     1561 ccgggggcgg aggcggggag tagccctgca cgccggggtt cggggtggcc cgcggcgcct
     1621 ccgcggcggg gcgcccgtga gatccgcccg cggctcgccg ggagcctcgc ggcgatacca
     1681 atccgatcgg agcgacggtg ctcccgtggc agcagccaca cacgagatgg ggcatatctc
     1741 cggcgatttt acacgaggcc ttgacttgac gacggccagt cgtagagtcg gtccgagccg
     1801 tccaggcggc acacggtccg cgtgaagggt cgaacccacc tgctccgtag cgacgaagga
     1861 accaccttcc gcccgattcc gcggacaccg ggcacatgga aaggtggtgc gccatgcccc
     1921 tgccggatcg tcccagcctc gagttcctga agaagctcgc caaggatcgg ctcccggaaa
     1981 tgcgccgcac cgctccgtcc gcttgcctcg cggacgccca gctcgcggtc gcgcgcgagc
     2041 acggcttccc gagctggcgc gcgctgcgcg ccgagctgca ccggctcgcc gctgctgcgc
     2101 ccgacccccc cgacctcgtc gtccagctct tcgccgcgat ccgtagcggc gaggggcccg
     2161 tggtggaccg gttgctcgcc gcccataccg gcctcgccaa cgcgcgcgac gaggagggca
     2221 gcaccccgct gtcggccgcg gtcgacgcgc atcgcgccgc gctcatcccg atcctgctgc
     2281 gccgcggagg ggatccccac ctggtgtacg cccactccgc ccacaccccg ctctcctggg
     2341 cggtggtgat cgaggcctac gactgcgccc gcgctctcat ggaggccggt gtcgagccgg
     2401 acctgttctg tgccgccggg ctgggcgagg tggaccgcgt gcgcaccttc ttcagccccg
     2461 agggccggct aaggccgcgg gcctcgatca ccggcagctc gcgctacgcc ccggacggca
     2521 cgcggctgcc gtgcccgccg gcgaccgacc gcgaggtcgt gtcggacgcg ctctacatgg
     2581 ccagccgcgc cggccacgag ggcacggtgc gggagctgct gacgcacggg cccgacctcg
     2641 cgttcccggc cttcgcgggc gccaccgcgc tgcactgggc ccattttgcg ggcgcccccc
     2701 gggtggtcgc gctcctgctc gcggcggggg cggatcccac gctccgcgat cccgtgctag
     2761 gctgcacgcc ggaggccttc ggcatctgca cccccgcgag ctgggcctgg ggctcgaagg
     2821 tgcgccagcg gctcgccgag aacccggggc tggtccacgc ggagagcacg cgcggagggc
     2881 ccctgcacga ggcggcgtgg gccggcgccc tggaggcggt gaagctgctg ctcgaggcgg
     2941 gggccgatcc ggcgcggcgc aacgcggagg gcaagaccgc gctcgatctg gcgcgggagc
     3001 ggccggatca gccctggtgc gtgaaggtgg tcgagtggct gacgagcagt gcacccgaac
     3061 cgaggagatg acgatgttct tcgagctacg gcagtatcac gtccgtcccg ggcagcagga
     3121 gaagtgggtg aagtgcatgg aggaggagat catccccttc caggtcaaga tgggcatggt
     3181 cgtcctcggc agcttcgtgg gcgaggagga cgcgtcggtg tacgtgtgga tccgccgctt
     3241 cgagagcgag ccggagcgca agcggctcta cgacgcggtc taccagagcg actactggaa
     3301 gaacgagatc tcgccgcggg tcccgcagat gatcgaccgc gagcagatca aggtcacgcg
     3361 catcgtcgcc acgccgcact ccgtgatcca gtgagcgtcg gccggtgagc caggccccgc
     3421 tcgaggcggt ccgcgcgctc gggcccctga tccgctcctc cgccgcggag acggacgcgc
     3481 agcgcgagct ggcgcgcccg ctcttcgagg cgatggcgga cgcggggctc ttccagctcg
     3541 ggctaccccg ggcggtgggc ggggcggagc tcgacctgcc gagctacatc caggtcatcg
     3601 aggagatcgg caagggcgac gcgagcaccg gctgggtgac caaccaggtg gcgatcttcg
     3661 cgacgtacgc ggcgcggatg ccgcgcgagg cggcgcgcgc catctggatc gacacgcccc
     3721 gcagcgtggt cgccaacacc ccgatggcca gcgcgcaggc ggtggtggtg ccgggcggct
     3781 accgggtcac cggccggcag ggcttcagca ccggctgccg gcacgcctcg tggctggccg
     3841 cccacgccac cgtgatggag cagggccgcc cgcgcctcga cgagggccag cccgagctgc
     3901 gctactgctt cgtcccgcgc gcccaggccg agctgctcga cacctggcag gtgcgcggca
     3961 tgcgggggac cgggacccac cacttcgcgg tggacgacgt cttcgtgccc gaggagcgca
     4021 ccgtgaagtc ggtgaccgcg ccgctgatcg agaccgggcc gctctaccag
//
LOCUS       DATMCV010000202         5410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_278342, whole genome shotgun sequence.
ACCESSION   DATMCV010000202 DATMCV010000000
VERSION     DATMCV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278342"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2015)
                     /locus_tag="VNU03_04690"
     CDS             complement(<1..2015)
                     /locus_tag="VNU03_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWP73783.1"
                     /translation="MTRRVTASLRGLVVLLALLRAAPTAAQDQAPASPPAFDAILPPV
                     LVTAPPALSSSSEQLIPGKDFELRPQGRPADVLRLIPGLIINQHQGGGKAEQYLIRGF
                     DADHGTDLALFVDGLPVNLRSHAHGQGYADLHFLIPETVKTVDVLKGPYWAEYGDFDT
                     AGVVSFVTRDHVEENTLEVAGGSFNTQRYLALLSPSRGALKTFIAADLYHNDGPFDHA
                     NGYIRFNLFAKASADIAEDMRLAVSASHYRAEWHGSGQIPARAVRSGLIDRFGAIDPT
                     EGGVTQRTNLNVEYTWKPTENQKLSAHAYLTYYELSLFNDFTFFLNDPTLGDEINQRD
                     RRFLAGFDTQYEIKSRPAGIPVTTTAGFQYRIDTPHVVLANATQRHQTGRVQDVRIVE
                     QSYSPFLKFDIAPVEKVRLVTGARGDVFSYQGTQQVNTVEGDLSGRVTRARPNVKANL
                     VLGPWVETEWFANFGTGYHSNDARAVLADPKLDALPTARGYEFGLRSRLVPRVELFAT
                     YWFLDLGSELVFVGDDGTTSAQGPSHRDGLELGAKVRLLDWLTFTGDFTYTRKAEFTR
                     TGEAVPLAPLWTARADLTARLPWGLASSFEMRYLGDRFADPDRHQTARGYTIFNWTTR
                     YRYKSLDAFLSIENLTNTQWREAQFFFTSRLRGEPAGGVDDIHFTPGT"
     gene            2171..3073
                     /locus_tag="VNU03_04695"
     CDS             2171..3073
                     /locus_tag="VNU03_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HWP73784.1"
                     /translation="MNRKILWLFSGVLVVLFAGSVAFAQSAGPAGMKLYAFSSGGLTI
                     AKSALQSGAPSTQVTVPVGFFVVKHPKGNFVFDTGNNDKIISDPGYWGPFIKGLTPER
                     GPDVAIDTQLKKIGLSPDDIKYVAVGHMHLDHGGNVCKFPKAIFLVQRDEMKNAAWPE
                     PGTAGPYIPGDVACLRNDLGESLPNKFKMDQLTGDLDVFGDGSVVLKAWSGHTPGSQM
                     AIVRLPKTGTVVLTSDNVYFSENVTKNLLPDISLAYNPTGILNAYEYIRMLQGRENAS
                     FMTAHDPDGPAGKARTTHAPQVFE"
     gene            3154..4098
                     /locus_tag="VNU03_04700"
     CDS             3154..4098
                     /locus_tag="VNU03_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73785.1"
                     /translation="MPPVIVADQLTKWYGPRLAVDRVSLEVGAGEVMGLLGPNGSGKT
                     TILRILTGYLRPSAGSARIAGLDVVHDSLAARARVGYVPEDAPLYGWMSVREFLVFMA
                     RIKGLSRQAAPSVVGGVVERLALGDVQGLLIGKLSRGYRQRVAIGQALLGDPALLILD
                     EPTNGLDPRQIIEMRGHIRSLAGERTVVVTSHILGEIERVADRVAILLAGRLLGVHAV
                     RGGELGRRFRLRVRGAEAAVEACLRGVPGVLGVRVEREPGADLASYFVDTASVPTAEA
                     LAAATVAGGFGLHEIAPAPVDLEALFLGLTSGGPEAAA"
     gene            4095..4796
                     /locus_tag="VNU03_04705"
     CDS             4095..4796
                     /locus_tag="VNU03_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP73786.1"
                     /translation="MRHFGTLLLKEERAVFASPIAYATVAVYLLLMGYTFTAVLFLNR
                     TGELVRVFFQAAVLFLLIVPVVTMRLFAEERRTGTLELLLTSPVREIEIVLAKFVAGL
                     TLPALMLALTGSYAIVLGLYGDPDWGPIYSGFLGLLLLAATLTAIGLLVSGLVTNQIV
                     AAVVAMGAGILLWMIDTLGYLLPDPFDGIVTHLSLVAHFSPFATGALFLSDVGFFLTI
                     ILAGLFLSVRALARR"
     gene            4799..>5410
                     /locus_tag="VNU03_04710"
     CDS             4799..>5410
                     /locus_tag="VNU03_04710"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HWP73787.1"
                     /translation="MDRLDVVNTLWFGGWLVALAVLFALGLRLPLQPRLRRLPALGYV
                     VGIVVATLGLVALANVALVLHDAHFDLTREGVFTPSKQAETVVDRLGRDVKLTYFYQS
                     QDQEGRRAKDMVEVLGRRNPRLHVRTVDPDKQPTVADTYGVRIYNAAVLEMDGRRIQV
                     MSTDEDQIALGILRVTREQVTTVCFMEGHGEYPMENFEFHTHFE"
BASE COUNT          776 a         1839 c         1902 g          893 t
ORIGIN      
        1 gtgcccggcg tgaagtggat gtcgtccacc ccgccggcgg gctctccccg gaggcgcgag
       61 gtgaagaaga actgggcttc ccgccactgg gtgttcgtga ggttctcgat gctgagaaac
      121 gcgtcgaggc tcttgtagcg atagcgggtg gtccagttga agatggtgta gccgcgggcg
      181 gtctggtgcc ggtcgggatc cgcgaaccgg tccccgaggt agcgcatctc gaagctcgac
      241 gcgagccccc agggtaggcg cgcggtgaga tccgcccggg cggtccagag cggcgcgagg
      301 gggacggcct ccccggtccg cgtgaactcc gccttccgcg tgtaggtgaa gtcgccggtg
      361 aaggtgagcc agtcgagcaa ccggaccttc gcgccgagct ccaggccgtc gcggtggctc
      421 ggcccctgcg cgctagtggt gccgtcgtcc ccgacgaata ccagctcgct gcccaggtcc
      481 aggaaccagt aggtggcgaa cagctcgacc cggggcacca gccggctgcg cagcccgaac
      541 tcgtagcccc gcgcggtcgg gagcgcgtcg agcttcgggt ccgcgagcac cgcacgcgcg
      601 tcgttgctgt gatagccggt cccgaagttg gcgaaccact cggtctccac ccatggaccc
      661 aggaccaggt tggccttgac gttcgggcgg gcccgcgtca cccggccgct cagatcgccc
      721 tcgacggtgt tgacctgctg cgtgccctgg tagctgaaga cgtcgccgcg ggcgccggtg
      781 accaggcgca ccttctcgac cggcgcgatg tcgaatttca gaaacggcga gtaggactgc
      841 tccacgatgc gcacgtcctg gacgcgcccg gtctggtgcc gctgcgtcgc gttggcgagg
      901 acgacgtgcg gggtgtcgat gcggtactgg aacccggcgg tggtcgtgac cgggatcccg
      961 gccggccgcg acttgatctc gtactgggtg tcgaagccgg ccaggaagcg ccgatcccgc
     1021 tggttgatct cgtcgcccag cgtcgggtcg ttcaggaaga acgtgaagtc gttgaacagg
     1081 ctgagctcgt agtaggtcag gtaggcgtgg gcggacagct tctggttctc ggtcggcttc
     1141 caggtgtact cgacgttcag gttggtgcgc tgggtgaccc cgccctcggt cgggtcgatc
     1201 gccccgaatc ggtcgatgag gccggaccgg accgcgcgcg cggggatctg cccggagccg
     1261 tgccactcgg cgcgatagtg agacgccgac accgccagcc gcatgtcctc cgcgatgtcg
     1321 gcgctcgcct tcgcgaagag attgaagcgg atgtacccgt tggcgtggtc gaacggcccg
     1381 tcgttgtgat acaggtcggc cgcgatgaag gtcttgagcg ccccgcggct cggcgagagg
     1441 agggccagat accgctgggt gttgaagctt ccgccggcca cttccagcgt gttctcctcg
     1501 acgtggtccc gggtgacgaa gctcaccacc ccggcggtgt cgaagtcgcc gtactcggcc
     1561 cagtacggcc ccttcaggac atccaccgtc ttgaccgtct ccgggatcag gaagtgcagg
     1621 tcggcgtagc cttgcccgtg cgcgtggctc cggaggttga ccggcaggcc gtccacgaac
     1681 agggccaggt cggtgccgtg gtcggcgtcg aagccccgga tcaggtactg ctcggccttg
     1741 cccccgccct ggtgctgatt gatgatcagc cccgggatca ggcggaggac atcggccggc
     1801 cggccctgcg gccgcagctc gaagtccttg ccggggatga gctgctccga ggaggacgag
     1861 agcgccggcg gcgcggtgac gagcacggga ggcaggatcg cgtcgaaggc ggggggcgat
     1921 gccggcgctt gatcctgtgc cgcggtcggc gcggcccgga gcagggcgag caggacgacc
     1981 agacctctca atgaagccgt gacgcgtctg gtcatcgggc gcccgtagta tcacgaggcg
     2041 gcggttgctt gacaagggtc tcgccgggcg cctatattcg gcgcgcgagg tcggcagcac
     2101 gccgatgaga gaagatgcgc gccacgtcgt cgcactgcag cgagcgatcg gatccgtgag
     2161 gaggggccgc atgaatcgga agatcctctg gttgttctcg ggagtcctcg tagttctctt
     2221 cgccggctcg gtggcgttcg cgcagagtgc cgggccggcc ggcatgaagc tctacgcgtt
     2281 cagctccggc gggctgacga tcgcgaagag cgcgctgcag tcgggggcgc cgagcacgca
     2341 ggtcacggtg ccggtcggct tcttcgtggt caagcacccg aagggcaact tcgtcttcga
     2401 caccgggaac aacgacaaga tcatcagtga tcccggctac tgggggccct tcatcaaggg
     2461 cttgaccccg gagcgcggcc cggacgtcgc catcgacacg cagctcaaga agatcggcct
     2521 gtcccccgac gacatcaagt acgtggcggt cggccacatg catctggacc acggcgggaa
     2581 cgtctgcaag ttcccgaagg cgatcttcct cgtgcagcgc gacgagatga agaacgcggc
     2641 gtggccggag ccggggaccg cggggccgta cattccgggt gacgtggcct gcctccgcaa
     2701 cgacctcggc gagtcgctgc ccaacaagtt caagatggac cagctgaccg gtgacctgga
     2761 cgtcttcggg gacggcagcg tcgtgctgaa ggcctggtcg ggtcacaccc cgggaagcca
     2821 gatggcgatc gtccgccttc ccaagaccgg gacggtcgtg ctgaccagcg acaacgtcta
     2881 cttctcggag aacgtgacga agaacctgct gcccgatatc tcgctcgcct acaacccgac
     2941 cggcatcctg aacgcgtacg agtacatccg gatgctccag ggccgtgaga acgcgagctt
     3001 catgaccgcc cacgatccgg acggccccgc cggcaaggcg cggaccacgc acgctccgca
     3061 ggtcttcgag taggtcacac gaccacgcag tccaggtagg acgcgaccgt cggccggccc
     3121 ggccggccga cgtcgcggtg gtcgctgccc gccatgcccc cggtcatcgt cgccgaccag
     3181 ctcaccaaat ggtacgggcc ccgactggcc gtcgaccggg tatctctcga ggtcggcgcg
     3241 ggcgaggtca tggggctgct cggcccgaac ggctccggca agaccacgat ccttcgaatc
     3301 ctcaccgggt acctgcggcc gtccgccggc agcgcccgca tcgccgggct cgacgtcgtg
     3361 cacgactcgc tcgcggcgcg cgcccgggtc ggctacgtgc ccgaggatgc gccgctctac
     3421 ggctggatga gcgtccgcga gttcctcgtg ttcatggccc gcatcaaggg cctctcgcgc
     3481 caggccgcgc cctccgtggt gggaggcgtg gtcgagcggc tggcgctcgg ggacgtccag
     3541 gggctcctca tcggcaagct ctcgcgcggc taccggcagc gcgtggccat cggccaggcc
     3601 ctgctgggcg atccggccct cctgatcctc gacgagccca ccaacggcct cgacccgcgg
     3661 cagatcatcg agatgcgcgg acacatccgg tccctggccg gtgagcgcac cgtcgtggtc
     3721 acctcccaca ttctcggcga gatcgagcgg gtggccgacc gggtggcgat cctgctcgcg
     3781 ggacggctgc tcggcgtgca cgcggtgcgg ggcggggagc tcggccggcg cttccggctg
     3841 cgggtgcgcg gagcggaggc ggccgtcgag gcctgtctcc ggggcgttcc cggcgtcctc
     3901 ggcgtgcggg tcgagcgcga gccgggggcc gacctcgcga gctacttcgt ggacaccgcg
     3961 tcggttccga ccgcggaggc gctggccgcc gcgacggtgg ccggcggctt cggcctccac
     4021 gagatcgcgc ccgcgccggt cgatctggag gccctgttcc tcggcctcac gagcggcggg
     4081 ccggaggcgg ccgcgtgagg cacttcggca cgctcctgct caaggaggag cgggcggtct
     4141 tcgcgtcccc catcgcctac gccacggtgg cggtctatct gctcctcatg ggctacacct
     4201 tcaccgcggt cctcttcctg aaccggaccg gcgagctcgt ccgggtcttc ttccaggccg
     4261 cggtgctctt cctcctcatc gtgccggtgg tgaccatgcg gctcttcgcg gaggagcgga
     4321 ggacggggac gctcgagctg ctcctgacct cgccggtgcg agagatcgag atcgtcctgg
     4381 ccaagttcgt ggccggcctg acgctgcccg ccctgatgct cgcgctcacc ggcagctatg
     4441 cgatcgtcct cggcctctac ggcgaccccg actgggggcc gatctacagc gggttcctgg
     4501 ggctcctgct tctggccgcc acgctcaccg ccatcgggct cctggtgtcc gggctcgtga
     4561 cgaatcagat cgtggccgcg gtggtggcga tgggcgccgg gatcctgctc tggatgatcg
     4621 acaccctcgg ctacctgctg cccgatccct tcgacgggat cgtcacccac ctgtcgctgg
     4681 tcgcgcactt ctccccgttc gcgaccggcg ccctgttcct ctccgacgtg ggcttcttcc
     4741 tcacgatcat cctggccggc ctcttcctga gcgtccgggc cctggcgcgg cggtagggat
     4801 ggaccgcctc gacgtcgtca acacgctctg gttcgggggc tggctcgtgg cgctcgcggt
     4861 ccttttcgcg cttgggctgc ggctccccct gcagccgcgc ctccgccgcc tgcctgcact
     4921 gggctacgtg gtcggcattg tcgtggctac cctcgggctc gtggccctcg ccaacgtggc
     4981 cctcgtgctc cacgacgcgc atttcgatct gacccgcgaa ggagtcttca cgccctcgaa
     5041 gcaggccgag actgtcgtcg accggctggg ccgcgacgtg aagctcacct acttctacca
     5101 gtcgcaggat caggaggggc ggcgggcgaa ggacatggtc gaggtgctcg ggcgccgcaa
     5161 cccccgcctc cacgtccgga ccgtcgaccc cgacaagcag ccgaccgtcg ccgataccta
     5221 cggggtgcgg atctacaacg cggccgtgct cgagatggac gggcgccgga tccaggtgat
     5281 gagcaccgac gaggaccaga tcgcgctcgg cattttgcgg gtgacgcggg agcaggtgac
     5341 caccgtctgc ttcatggaag gccacggcga gtaccccatg gagaacttcg agttccacac
     5401 ccacttcgag
//
LOCUS       DATMCV010000203         2977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_146180, whole genome shotgun sequence.
ACCESSION   DATMCV010000203 DATMCV010000000
VERSION     DATMCV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146180"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VNU03_04715"
     CDS             <1..555
                     /locus_tag="VNU03_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HWP73788.1"
                     /translation="QDEQRLRQSIGSYQTRLDNAPKREQEYLDATRDYQGTKELYQTL
                     SRKHDDALLSESMEQRQKGEQFRILDSALPSGTPAAPRRSRLLIAGLALSFVLGAGSM
                     VVMELLDTSFHSARDLRAYTTVPILVNIPRIVTENDARRRRWRFRFAAVGVLIALLVV
                     GGSSYFVAHGNEQLAQLLSRGDRA"
     gene            563..1609
                     /locus_tag="VNU03_04720"
     CDS             563..1609
                     /locus_tag="VNU03_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP73789.1"
                     /translation="MALYLNFYGFDRDPFGVTPDPDFLFMTPGHKEALAQLLYAIQEK
                     KGFILVTADIGLGKTTLLEALRQELDSNTAVAYVSNSLLPFPAILEYMLEEFEIEKPG
                     ESAAQRLVVLQNFLVERHGAGQNTVLILDEAQNLYPQTLEQIRLLSNFETTKEKILQI
                     VLVGQPELLARLEMPELRQLKQRISIMCTIPPMTRSGTRNYIRTRLRMAGARDLGIFT
                     DDAVEHIVAYANGIPRVVNTVCDHCLLIGYADQTRRIDRGIVNQAIGYLEAGHRPRVR
                     TRSSLGARRARARSKPSTVSPLRWGLLGMSAALVSGVAATVFFHPDLLPTAVDISTSY
                     LTDLARSVRTLIPR"
     gene            1606..2688
                     /locus_tag="VNU03_04725"
     CDS             1606..2688
                     /locus_tag="VNU03_04725"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HWP73790.1"
                     /translation="MSKFSKALEQAQRDRAARLPPAPAPPTPHVPQAPAAPPVSQAPP
                     PPPPPIPLTAPLPVTSAPPNGATLPPGAPRRRPATGPRRTATITGEVENHLVSLVSPS
                     GLEAEQYRALRHVVEQRHKEQDLSVIAISSPGVGDGKTTTSINLAGALAQGADNSVLL
                     IEADLRRPSIGHLLGFNGSRTVGLVDAILDPSLTLESVVQTRPPFNLSVVLAGQIPAS
                     PYEVLKSPRLGALLDEARAQYDFIVMDTPPLALVQDCRVVARWVDGVVVVVAAHQTPR
                     SLLETALDVVDPSKLVGIVFNGFDNLGGGHHGRHYAGYYATPETGPEEAARSGALTRI
                     SQKVGSMLRRGEDAAVRNRRSRDRSR"
     gene            2685..>2977
                     /locus_tag="VNU03_04730"
     CDS             2685..>2977
                     /locus_tag="VNU03_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73791.1"
                     /translation="MILAIIEGLVLSLAVTGTAFAWGHRLFVDWLDVATMLGQAAAVS
                     LCCITAFYYNDLYDLQVVRSFSLFASRLLQSFGVALMLLAVFYTIIPDAGMTEG"
BASE COUNT          448 a         1123 c          941 g          465 t
ORIGIN      
        1 caggacgagc agcggctgcg gcagagtatc ggcagctatc aaacccgtct ggacaacgcc
       61 ccgaagcgcg agcaggaata tctcgatgcc acgcgggact atcaaggcac caaggagctc
      121 taccagactc tctcccgcaa gcacgacgac gcccttctgt cggagagcat ggagcagcgg
      181 cagaagggcg aacagttccg gatcctcgac tcggcgctgc cctcgggcac accggcggcg
      241 ccgcgccgct cgcggctcct gatcgcgggc cttgccctct cgttcgtact cggcgcgggc
      301 tccatggtgg tgatggagct gctcgacacc tcgttccatt ccgcccggga cctccgcgcg
      361 tacaccacgg taccgatcct ggtgaacatc ccgcgcatcg tgaccgagaa cgacgcgcgc
      421 cggcgccgct ggcgcttccg cttcgcggcg gtcggggtgc tgatagcgct cctcgtcgtc
      481 ggcgggtcct cgtacttcgt cgcgcacggc aacgagcagc tcgcccagct gctctcccgg
      541 ggagatcggg cctagcgccg ccatggctct ctacctgaac ttctacggat tcgatcgaga
      601 cccgttcggg gtcacccccg atcccgattt cctgttcatg acccctggcc acaaggaggc
      661 gctcgcccag ctcctctacg ccatccagga gaagaagggc ttcatcctgg tgacggccga
      721 catcggcctg ggcaagacga ccctgctgga ggccctgcgc caggagctcg atagcaatac
      781 cgcggtggcc tacgtctcca actcgctgct ccccttcccg gccatcctcg agtacatgct
      841 ggaggagttc gagatcgaga agccgggcga gtctgcggcg cagcgcctgg tggtcctgca
      901 gaacttcctg gtcgagcggc acggcgccgg ccagaacacg gtgctcatcc tcgacgaggc
      961 gcagaacctc tacccgcaga cgctcgagca gattcggctc ttgtcgaact tcgagaccac
     1021 caaggagaag atcctgcaga tcgtgctcgt cggccagccg gagttgctcg cccgcctgga
     1081 gatgccggag ctgcgccagc tcaagcagcg catctcgatc atgtgcacga tcccgccgat
     1141 gacccgctcc ggtacccgga actacattcg gacccggctg cggatggcgg gcgcccggga
     1201 ccttggcatc ttcacggacg acgccgtcga gcacatcgtc gcgtacgcga atggcattcc
     1261 gcgcgtggtc aacacggtct gcgaccactg cctgctgatc ggctacgcgg atcagacccg
     1321 tcggatcgac cgcggcatcg tgaaccaggc catcggctat ctcgaggccg gccaccggcc
     1381 gcgcgtccgc acgcgcagca gcctcggcgc ccggcgggcc cgtgcccgta gcaagccgag
     1441 cacggtcagc ccgctgcgct gggggctcct cggcatgtcg gcggccctgg tgagcggcgt
     1501 cgcggccacc gtgttcttcc atccggatct gctgcccacc gcggtcgaca tctccacctc
     1561 gtacctcacg gacctggccc ggtcggtccg caccctgatc ccgcgatgag caagttctcg
     1621 aaggccctcg agcaggccca gcgcgaccgg gcggcgcgcc tgccgccggc gcccgcgccc
     1681 ccgactcccc acgtgcccca ggcaccggcc gctccgccag tgtcccaggc gccgcctccg
     1741 ccgccacccc cgatcccgct gaccgcaccg ctgcccgtga cctcggcgcc gccgaacggc
     1801 gccacgctgc cgcccggggc cccgcgccgg cgcccggcca ccgggccgcg ccgcaccgcc
     1861 accatcaccg gcgaggtcga gaaccacctg gtgagtctcg tctccccgtc cgggctcgag
     1921 gccgagcagt accgggccct gcgtcacgtg gtggagcagc ggcacaagga gcaggacctg
     1981 tcagtgatcg ccatctcgag ccccggcgtc ggcgacggca aaaccacgac gtcgatcaat
     2041 ctcgcgggcg cgctcgccca gggggccgac aactcggtgc tgctgatcga ggccgatctg
     2101 aggcggccgt ctatcggcca cctgctcggg ttcaacggct ctcggaccgt cggcctggtg
     2161 gatgcgatcc tcgatccgag cctcacgctc gagagcgtcg tgcagacccg gcctccgttc
     2221 aacctgagcg tggtcctggc tggccagatc ccggcgagcc catacgaggt cctgaaatct
     2281 ccgcggctcg gcgcgctcct cgacgaggca cgcgcccagt acgatttcat cgtcatggac
     2341 acgccgcccc tggcgctggt ccaggactgc cgcgtggtcg cccgctgggt cgacggcgtg
     2401 gtggtggtcg tggcggcgca tcagacgccg cgctcgctgc tggagaccgc gctcgacgtc
     2461 gtcgacccca gcaagctggt cggcatcgtg ttcaacggct tcgacaacct gggcggcggc
     2521 caccacggcc gccactacgc cggctactac gccacgccgg agaccggccc cgaggaggcc
     2581 gcgcgcagcg gcgccctcac ccggatcagc cagaaggtcg gctccatgct ccgacgcggc
     2641 gaggacgccg cggtgaggaa ccgccgctcg cgggaccgtt cgcggtgatt ctcgcaatca
     2701 tcgaggggct ggtgctctcg ctggcggtca ccgggaccgc cttcgcatgg ggccaccgtc
     2761 tcttcgtcga ctggctcgac gtggccacca tgctcggcca ggccgcggcg gtttccctct
     2821 gctgcatcac cgccttctac tacaacgacc tctatgacct ccaggtcgtc cgcagcttca
     2881 gcctcttcgc ctcccgcctg ctgcagtcct tcggcgtcgc gctgatgctg ctggccgtct
     2941 tctacacgat catccccgac gccggcatga ccgaggg
//
LOCUS       DATMCV010000204         2954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_345146, whole genome shotgun sequence.
ACCESSION   DATMCV010000204 DATMCV010000000
VERSION     DATMCV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345146"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..959)
                     /locus_tag="VNU03_04735"
     CDS             complement(123..959)
                     /locus_tag="VNU03_04735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP73792.1"
                     /translation="MHIGVCMFATDYAIRADELARAAEERGFESLWVPEHTHIPVSRR
                     TPFPGGAQLPKEYSHTYDPFVSLMAAAAATKRLKVGTGICLIIERDTITTAKEVASLD
                     ALSGGRFLFGIGAGWNAEEMENHGTVFKTRFHRMREQVLAMKEIWTKDEAQYHGQHVN
                     FDPIWAWPKPAQKPHPPVLLGGESGHTLQRVVDFCEGWFPRGRAGDVILAGLKDLQAR
                     AARAGRDMKTISVSVFGAKPDEATLETYAGAGITRSILRLPSEGRDVILPLLDQYAKL
                     IH"
     gene            complement(977..1480)
                     /locus_tag="VNU03_04740"
     CDS             complement(977..1480)
                     /locus_tag="VNU03_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP73793.1"
                     /translation="MSVALIREHYDYHHWANRRLFGLAVDLGDGVTRDMGQHWSFPTL
                     KGMFAHICGADDAWLKRWKGASPGRILGDGDFATVADLRTRWDALEAEQRAFLAGLGE
                     ADLARPVVYKNTQGQEFRVALGPLLQHVVNHATHHRSEAATMITMISGSPPDTGINTY
                     RTAVVKG"
     gene            complement(1503..1718)
                     /locus_tag="VNU03_04745"
     CDS             complement(1503..1718)
                     /locus_tag="VNU03_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HWP73794.1"
                     /translation="MSEDVKAHITGVVFQVTTKPGDAVAAGDPVIVLESMKMEIPVEA
                     PRAGKVREIKVSEGQTVQEGDTVAVLD"
     gene            complement(1797..2591)
                     /locus_tag="VNU03_04750"
     CDS             complement(1797..2591)
                     /locus_tag="VNU03_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73795.1"
                     /translation="MALALTEPGAGSDAANLACRAERDGDHYVITGEKSGISLGMVAQ
                     AAIVFARTDPAGRARGVSAFLVPLDASGVSRSPLRDMGTRAIARAVLAFDHVRVPASH
                     RLGEEGTGFYQVMQGFDYNRIGIALACLGVAQQSVEETIAYVKERKAFGRPIAQFEGV
                     SFPIAEAATHLEACRWLCYRALWLADQGRPHTKESAMTKWWGPRLSVDTIHQCLLLHG
                     HYGYTDELPFEQRMRDVIGLEIGDGAAEVMKMVVARELLGRESLPY"
     gene            complement(2727..>2954)
                     /locus_tag="VNU03_04755"
     CDS             complement(2727..>2954)
                     /locus_tag="VNU03_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73796.1"
                     /translation="AVMDLAFTPEQDELIRILRQFARRDLAPHSAEWDRSGQFPWEAW
                     GRMGELGLLGLRVPVAYGGQESDFVTYGIAM"
BASE COUNT          420 a         1038 c         1025 g          471 t
ORIGIN      
        1 gaaaactagg ccgggggtcg agctagaccg gggtcaggtc ttacattccg acatttcgga
       61 gcagcgcgag atcgcgcgcc gcgccagaaa atgtcggaat gtaagacctg accccttctg
      121 cgtcagtgga tcagcttggc gtactggtcg aggagcggga ggatcacgtc gcgcccctcg
      181 gagggtagcc gcaggatcga gcgggtgatg cccgcgcccg cgtaggtctc gagcgtggcc
      241 tcgtccggct tggccccgaa caccgagacc gagatcgtct tcatgtcgcg ccccgcccgc
      301 gcggcgcggg cctgcaggtc cttaaggccg gccaggatca cgtcgccggc ccggccgcgc
      361 gggaaccagc cctcgcagaa gtcgacgacc ctctgcagcg tgtgaccact ctccccgccc
      421 aggagcaccg ggggatgcgg cttctgggcc ggcttgggcc aggcccagat cgggtcgaag
      481 ttgacgtgct ggccgtggta ctgcgcctcg tccttggtcc agatctcctt catggccagc
      541 acctgctccc gcatccggtg gaagcgggtc ttgaagacgg tgccgtggtt ctccatctcc
      601 tccgcgttcc accccgcccc gatgccgaag aggaagcggc cgcccgacag cgcgtcgagg
      661 cttgccacct ccttggcggt ggtgatggtg tcgcgctcga tgatcaggca gatcccggtg
      721 ccgaccttca ggcgcttggt cgcggcggcc gcggccatca gcgagacgaa cggatcgtag
      781 gtgtgcgaat actccttggg cagctgcgcg ccgcccggga agggggtgcg ccggctgacc
      841 ggaatgtggg tgtgctccgg cacccacagg gactcgaacc cgcgctcctc ggccgcgcgg
      901 gccagctcgt cggcccggat cgcgtagtcg gtggcgaaca tgcagactcc gatgtgcatg
      961 actgtctcct ttcgggtcaa cccttcacga cggcggtgcg ataggtattg attccggtgt
     1021 cgggcgggga gccgctgatc atggtgatca tggtcgcggc ctcgctgcgg tgatgcgtgg
     1081 cgtgattgac cacgtgctgg agcaagggcc cgagcgcgac ccggaactcc tggccctggg
     1141 tgttcttgta cacgaccggg cgggcgaggt cggcctcccc gagcccggcc aggaaggccc
     1201 gctgctcggc ctcgagcgcg tcccagcgcg tgcggagatc ggccacggtg gcgaagtcgc
     1261 cgtcgcccag gatccgtccc ggcgatgcgc ccttccagcg cttgagccac gcgtcgtcag
     1321 cgccgcagat gtgggcgaac atccccttga gggtggggaa gctccagtgc tggcccatgt
     1381 cccgggtcac gccgtccccc aggtccacgg ccaggccgaa gaggcgtcga ttcgcccagt
     1441 ggtgatagtc gtagtgctcg cggatcagtg cgacggacat gctgtctcct cctccggacg
     1501 gctcagtcga ggaccgcgac ggtgtctccc tcctgcaccg tctgtccctc gctgaccttg
     1561 atctcccgca ccttgccggc gcgcggggct tccaccggga tctccatctt catggactcg
     1621 agcacgatga ccgggtcccc ggccgccacc gcgtcgcccg gtttggtcgt gacctggaag
     1681 accacgccgg tgatgtgagc cttgacgtcc tcagacattg ggcgctcctt gtcgtgccac
     1741 ggagggattt gaggcggggg cattctctca catccgggcg ggagcgggcg ggccgcctag
     1801 tagggcaggc tctcccggcc caggagctcg cgggccacca ccatcttcat cacctcggcc
     1861 gcgccgtcgc cgatctcgag cccgatgacg tcgcgcatcc gctgctcgaa cggcagctcg
     1921 tcggtgtagc cgtagtggcc gtgaagcagc aggcactggt ggatggtgtc gaccgagagg
     1981 cgcggccccc accacttggt catggccgac tccttggtgt ggggccgccc ctgatccgcg
     2041 agccagagcg cgcggtagca gagccagcgg caggcttcga ggtgcgtggc cgcctccgcg
     2101 atcgggaaag acaccccctc gaactgggcg atcggccggc cgaaggcctt ccgctccttc
     2161 acgtaggcga tggtctcctc gaccgactgc tgggccacgc ccaggcaggc cagcgcgatg
     2221 ccgatgcggt tgtagtcgaa gccctgcatg acctggtaga agccggtccc ctcctcgccc
     2281 agccggtgcg aggcgggcac ccggacgtgg tcgaaggcga gcaccgcgcg cgcgatggcg
     2341 cgcgtgccca tgtcccgcag cgggctgcgc gagacgccgg acgcgtcgag cggcaccagg
     2401 aacgcgctga ccccgcgggc ccggcccgcc ggatcggtcc gcgcgaagac gatcgcggcc
     2461 tgcgccacca tgccgaggct gatcccggac ttctcgccgg tgatcacgta gtggtcgccg
     2521 tcgcgctcgg cccggcaggc caggttggcc gcgtctgagc ccgcgcccgg ctcggtgagc
     2581 gccagggcca ccaccgactc cccgcgcgcg gtggggggca gccagcgcgt cttgatctcc
     2641 tccgacccgc tccgccccag gatctggccg gccaggcccg cgagctggat gccgtaggtg
     2701 caggcgaagt cgccgcgccc gatctcctac attgcgatgc cgtacgtgac gaagtcggac
     2761 tcctgcccgc cgtacgcgac gggcacgcgc aggcccagca ggcccagctc gcccatccgg
     2821 ccccacgcct cccacgggaa ctgtccgctc cggtcccact cggcggagtg cggggccagg
     2881 tcccgccggg caaactggcg caggatgcgg atcagctcgt cctgctcggg cgtgaatgcc
     2941 aggtccatca cggc
//
LOCUS       DATMCV010000205         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_477964, whole genome shotgun sequence.
ACCESSION   DATMCV010000205 DATMCV010000000
VERSION     DATMCV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477964"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..1103)
                     /locus_tag="VNU03_04760"
     CDS             complement(369..1103)
                     /locus_tag="VNU03_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWP73797.1"
                     /translation="MPPGRLTAPSSVETCLQRIDWEAIARGLGERGWATTPPLLTAAE
                     CAELVALYPEDGRFRSRVDMARFRFGEGEYKYFANPLPPTVTALRRHAYPRLAPIADA
                     WSVALGGKPTHPPDLDGFLAICAERGQTRPTPLLLRYEAGGYNCLHQDLYGAIAFPLQ
                     MTCLLSRPGADFTGGEFLLVEQRPRAQSRGEVVPLEQGQIVIFATRERPVRGARGVYR
                     VGLRHGVSRVTSGLRYALGVIFHDAE"
     gene            complement(1085..1816)
                     /locus_tag="VNU03_04765"
     CDS             complement(1085..1816)
                     /locus_tag="VNU03_04765"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWP73798.1"
                     /translation="MSDMDDGKRPDRLDLLLTDYFRPESAAAELPRRIAAAARESSVA
                     LERLEARLGIEATARGISRVGTGPVGKPATAAARKLVEQARLEIHEYLRGQRAFFRVP
                     VDLTTVPDFQKSVLQAAGHIPFGEARAYAWIAARVGHPRAVRAVGTALGRNPVPLILP
                     CHRVWRSDGGLGGYIFGADVKDRLVALERTTPVLEGCTSTRIVCRVGCIHGRHMRRDN
                     RVVFASVEDARSVGYRPCKVCRPAA"
     gene            complement(1809..2351)
                     /locus_tag="VNU03_04770"
     CDS             complement(1809..2351)
                     /locus_tag="VNU03_04770"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP73799.1"
                     /translation="MDAERGLMARVARGDQAAFTDLAQRYSGKLLAVAGRLLGSRADA
                     EDAVQRAFLQCFAAAAGYDPRWAVSTWLYRILTNVCVDELRRRATRTAHDDRSAAGEL
                     SAGSSNHHPPAAYLDLHRALEQVPREARVLLALRYVDGLSYGELARIRGISINTVKSQ
                     LARGKSLLRAELGKRGADHV"
BASE COUNT          387 a          964 c          862 g          348 t
ORIGIN      
        1 ctccgctact cgcactgttc ctgttcttct tgccgatcag cggcgcggga tacttcggat
       61 tgcaccacgg cccggcattc acaatttatc tcctgctggt gaatctgccc ttcgggatag
      121 tcaccgccct ggccgccaaa gccatcatag gaccgaagcc ctagccagcc ccgcccgcgg
      181 gtttcagaat cagatctaac aacctggtgg cggggggagg gaggggggac ggggggtgtc
      241 cgggacccgt cccctctggc gcaagccgag ccaccaacat cccagactgg gaactcctcc
      301 ccagaagtcc gcgcgagatg ggccgtggtc ccggacaccc cccgtccccc ctccctcccc
      361 ccgccacact actccgcgtc gtggaagatc accccgagcg cataccgcag ccccgatgta
      421 acccgactca cgccatgccg cagccccacc cggtacacgc cgcgggcgcc gcgcaccggc
      481 cgctcccgcg tcgcgaagat cacgatctgc ccctgctcga gcggcaccac ctcgccccgc
      541 gactgggccc ggggccgctg ctcgaccagc aggaactcgc cgccggtgaa gtccgcaccc
      601 gggcggctca ggaggcaggt catctggagc gggaacgcga tcgcgccgta caggtcctgg
      661 tgcagacagt tgtagccgcc ggcctcgtag cgcagcagca gcggcgtggg cctggtctgg
      721 ccgcgctcgg cgcagatggc caggaagccg tcgaggtccg gcgggtgggt gggcttgcct
      781 ccgagggcca cgctccatgc gtccgcgatc ggggccaggc gcgggtaggc gtggcgccgc
      841 aatgccgtca cggtcggagg cagcgggttc gcgaagtact tgtactcgcc ctccccgaag
      901 cggaagcgcg ccatgtcgac ccggctgcgg aaccggccgt cctccggata cagcgcgacc
      961 agctccgcgc actccgcggc ggtcagcagc gggggcgtgg tggcccagcc ccgctcgccg
     1021 agcccgcggg cgatcgcctc ccagtcgatc cgctgcaaac aggtctcgac cgacgacggg
     1081 gcggtcaggc ggccgggcgg catactttgc acggccggta acccaccgac cgcgcgtcct
     1141 ccaccgaggc gaagaccacc cggttgtccc gccgcatgtg ccgtccgtgg atgcagccca
     1201 cccggcacac gatgcgcgtg ctcgtgcagc cctccagcac cggagtggtg cgctcgagcg
     1261 cgaccagccg atccttgacg tccgccccga agatgtagcc gccgaggccg ccgtcgctcc
     1321 gccacacccg gtggcacggc aggatgagcg gcaccgggtt gcggccgagt gcggtgccca
     1381 ccgcccggac cgcgcgcgga tggccaacgc gcgcggcgat ccacgcgtag gcgcgcgcct
     1441 cgccgaacgg gatgtgcccc gccgcctgca gcacgctctt ctggaagtcc ggcacggtcg
     1501 tgagatccac cggcacccgg aagaaggcgc gctgtccgcg caggtactcg tggatctcga
     1561 gacgggcctg ctcgaccagc ttccgcgccg cggcggtggc cggcttgccc accggcccgg
     1621 tgcccacccg cgagatcccg cgcgcggtcg cctcgatgcc gagccgcgcc tcgagccgct
     1681 cgagcgcgac gctggactcc cgggccgcgg ccgcgatgcg tcggggcagc tccgccgccg
     1741 ccgactcggg ccggaagtag tccgtcagca ggaggtccag ccgatccggt cgcttgccgt
     1801 cgtccatgtc agacatgatc cgctcctcgc ttgccgagct cggcccgcag cagggacttg
     1861 ccccgcgcga gctggctctt cacggtgttg atcgagatgc cccggatgcg cgccagctcc
     1921 ccgtaggaga gcccgtcgac gtagcgcagg gccagcagca cccgcgcctc gcgcggtacc
     1981 tgctcgaggg cccggtgcag atccagatag gccgcgggcg ggtggtgatt gctcgagccg
     2041 gccgagagct cccccgccgc cgaccgatcg tcgtgggcgg tccgggtagc ccgccggcgc
     2101 agctcgtcca cgcagacatt cgtgagaatg cgatagagcc acgtggagac cgcccaccgg
     2161 ggatcgtagc cggcggcggc ggcgaagcac tggaggaagg cacgctggac cgcgtcctcg
     2221 gcatcggcgc gggagccgag cagccgcccg gccaccgcga gcagctttcc gctgtagcgc
     2281 tgggccaggt cggtgaacgc cgcctggtcc ccgcgcgcca ccctcgccat cagcccccgc
     2341 tcggcatcca tctcacgccc tcaccgagat atacggccgg gaggcgaaaa cgggcggaag
     2401 ctcaaaaccc aaaaccgcca acgattgccc gccgactggg ggagtgcggg ggccatttct
     2461 gggcccccgc agctgaagag cctgaagatt taggcggtcg cccgccccca ggcctaaatc
     2521 ttctcgccga gcttgttcca gtcgtcgagg aacttcttga g
//
LOCUS       DATMCV010000206         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_146248, whole genome shotgun sequence.
ACCESSION   DATMCV010000206 DATMCV010000000
VERSION     DATMCV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146248"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VNU03_04775"
     CDS             <1..601
                     /locus_tag="VNU03_04775"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HWP73800.1"
                     /translation="SAAAARAAEKRRAESSRPRGDRAYEWLLSVLVPAAQLRILPYNR
                     VIRDVGGRSSQALLAELSACGTVRPVSEPVPPEPGMFCFYLERRWWRLDLPGGSMDRS
                     DPVRSLDVSLLQERVLGPVLGIVDQRTDPRIDFVGGIRGTQALEAQVDAGLAALAISL
                     YPTTVEQVMAVADRGHIMPPKSTWFEPKLLSGLFVHPLD"
     gene            613..906
                     /locus_tag="VNU03_04780"
     CDS             613..906
                     /locus_tag="VNU03_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWP73801.1"
                     /translation="MFARIGTWQGSPADLERWIVRGREQVKPAIRQDPGLTAVYWLVD
                     REAGKGMIVTLWESREAMAASEQARAARQAATSTATGAAVTTDRYEVVDWLTT"
     gene            1027..2325
                     /locus_tag="VNU03_04785"
     CDS             1027..2325
                     /locus_tag="VNU03_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP73802.1"
                     /translation="MSTISRRKFLGSAGLLGAGAVLADGARRSARADKPINFSGWVFK
                     PDTVKDYVEFYNSKHGGQVKYEAIPWATYHSTMETRAFAGEVVDVMYCNSANREHWFE
                     NGLIRALDDLPGMDELKKKIAPASLDSLKNRDGSKIIGLPYFNSVFILMYNEPMLQQA
                     GIKAPAKTWDELIEHSAKLKRDKVSDTPYLPNWNNSASGTMPQFLSDSFSEGANVFDA
                     KNRVIVDQEPGVARAMERWQKAYAAGLVNPEVLTKTSSTDTHRLFWTGRYAYHTNHSY
                     YLRTIAGEPENSKLAPKKAKIAMYPGNGQTHMFTEFYTVNAKTKALEDAWKFAKFLGG
                     NGNGDWYVQRQWCLIAGLDNPYPEMYDHPEIIQSYDKWIDLKLLREQYKKGRTINAFK
                     ESWFSEYDTKAIAIVHNIIRGSSTVPKGLKELVALQKSLV"
BASE COUNT          426 a          844 c          808 g          334 t
ORIGIN      
        1 ctcggccgcc gcggcgcggg cggcggagaa gcgccgcgcc gagagctcgc gcccgcgcgg
       61 cgaccgcgcc tacgagtggc tgctctcggt gctggtgccc gcggcccagc tccgcatcct
      121 gccgtacaac cgcgtgatcc gggacgtcgg cggccggtcc tcgcaggcgc tgctcgccga
      181 gctgtccgcc tgcgggacgg tccggcccgt ctccgagccg gtcccgcccg agccggggat
      241 gttctgcttc tatctcgagc gccgctggtg gcggctcgac ctgcccggcg gctccatgga
      301 ccggagcgac ccggtccgct cgctcgacgt ctcgctgctg caggagcggg tgctggggcc
      361 ggtgctcggc atcgtcgacc agcgcaccga tccccgcatc gacttcgtgg gtggcatccg
      421 gggcacccag gccctcgagg cccaggtcga tgcgggcctg gccgcgctcg cgatctcgct
      481 ctacccgacc accgtggagc aggtcatggc cgtcgcggac cggggacaca tcatgccgcc
      541 caagagcacg tggttcgagc ccaagctgct gagcgggctc ttcgtccacc cgctggactg
      601 acggaggctc ccatgttcgc ccgcatcggc acctggcagg gatcacccgc cgacctggag
      661 cgatggatcg tgcgcggccg cgagcaggtg aagcccgcca tccggcagga tccagggctc
      721 accgcggtct actggctcgt cgaccgggag gcgggcaagg ggatgatcgt gacgctctgg
      781 gagagccgcg aggcgatggc ggccagcgag caggcgcgcg cggcgcggca ggccgcgacg
      841 tccaccgcga ccggcgccgc ggtgaccacc gaccgctacg aggtcgtcga ctggctcacg
      901 acgtgaccga gctcgtcggg ctcccgtctt gacaatccga cgggcgctgc tccagaatcc
      961 cgccagcgct cgcggcccga tcgcccgtcg ccgccgacgt acccccacat acaggaggag
     1021 aaacccgtga gcacgatcag ccgacgcaag ttcctcggga gtgcgggatt gctgggagcg
     1081 ggcgcggtgc tcgccgacgg agcgcggcgg agcgcgcgcg cggacaagcc gatcaacttc
     1141 tcggggtggg tattcaagcc cgacaccgtg aaggactacg tggagttcta caacagcaag
     1201 cacgggggcc aggtgaagta cgaggccatc ccgtgggcca cctaccactc caccatggag
     1261 acgcgcgcct tcgcgggcga ggtggtcgac gtgatgtact gcaactcggc caaccgcgag
     1321 cactggttcg agaacggcct gatccgggcc ctcgacgacc tgcccggcat ggacgagctg
     1381 aagaagaaga tcgcgccggc cagcctggac agcctgaaga accgcgacgg cagcaagatc
     1441 atcgggctgc cctacttcaa cagcgtgttc atcctcatgt acaacgagcc gatgctgcag
     1501 caggccggca tcaaggcccc ggccaagacg tgggacgagc tgatcgagca cagcgcgaag
     1561 ctcaagcgcg acaaggtgag cgacaccccg tacctcccca actggaacaa cagcgcctcc
     1621 ggcaccatgc cccagttcct gagcgacagc ttctcggagg gcgccaacgt cttcgacgcc
     1681 aagaaccggg tcatcgtgga ccaggagccc ggggtggccc gggccatgga gcgctggcag
     1741 aaggcctacg cggcgggcct cgtgaacccg gaggtgctca ccaagacctc ctccaccgac
     1801 acccaccggc tcttctggac cgggcgctac gcctatcaca ccaaccactc ctactacctg
     1861 cggaccatcg cgggggagcc ggagaactcc aagctcgccc ccaagaaggc caagatcgcg
     1921 atgtaccccg gcaacgggca gacccacatg ttcaccgagt tctacaccgt gaacgccaag
     1981 accaaggcgc tcgaggacgc gtggaagttc gccaagttcc tgggcggcaa cggcaacggg
     2041 gactggtacg tgcagcggca gtggtgcctc atcgccggcc tcgacaatcc gtatcccgag
     2101 atgtacgacc acccggagat catccagtcg tacgacaagt ggatcgatct caagctcctg
     2161 cgcgagcagt acaagaaggg gcggaccatc aacgcgttca aggagtcgtg gttctcggag
     2221 tacgacacca aggccatcgc gatcgtccac aacatcatcc gcggctcatc gacggtgccg
     2281 aagggcctca aggagctggt ggcgctgcag aagtccctgg tgtaacccgc ggcgcccgcg
     2341 ggttccatgg tcaagagcgg cgcgggcggg ctccgtctct ccgaggcggg gttcgcctgg
     2401 ctgctcgtcc tc
//
LOCUS       DATMCV010000207         3900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_413069, whole genome shotgun sequence.
ACCESSION   DATMCV010000207 DATMCV010000000
VERSION     DATMCV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413069"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VNU03_04790"
     CDS             complement(<1..1302)
                     /locus_tag="VNU03_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HWP73803.1"
                     /translation="MKLRTFLLAVVGLTLVPLLGVAGLAIWWAHEDERRNMEQALLYH
                     ARSLTVAVDREVETSLAGLKGLATSTDLESGDLRNFYEEARLARDAYRRWLTVALVDP
                     SGRQVLNLLRPLGSPLPSVAALETFQRTLQTGEPQVSDLEIGPAAGRWVISVTLPLLR
                     AGKVHHILVAVMTPESFASVLAAADVAGATIVDRKGVVVATTEGPEQRVGTPASAGFV
                     SRAREQDEAVFTGGATHDGATAYTAFSRAKWSRFTVGIAVPRESLEGPLHRSLWLLSA
                     AAVGALVLSLGLTHLAGRRFAGRLRQLTAAFSAFGRGETVPELPDFTLSELSGVKGAL
                     RDAMALLQTRTAALEESERRYRTLFERNPAGMSLTLADGRIVDCNPAFARILGFESPA
                     DVLGTNVGTLYAQPKERDQLLERVAAEGTAINGELQMRRRDG"
     gene            1376..2836
                     /locus_tag="VNU03_04795"
     CDS             1376..2836
                     /locus_tag="VNU03_04795"
                     /inference="COORDINATES: protein motif:HMM:NF006560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP73804.1"
                     /translation="MKYRSLVLLCLVAVLPLSTSIRPAQSRTATFDLVLTGGRVIDPA
                     SWLDGVRNIGISNGTVAAISTGPLSGKQTIDVANLVVAPGFIDMHAHGQNVPSQTYQV
                     RDGVTTALELEIGAFPLNALGAREGRALINYGYSAGHTAARITVKRGNRQAAYHEALT
                     ESELKEMIGYLATALDEGALGIGVGLDYVSLGVKPDELEALFRLAAARKVPIFVHIRM
                     PDDRNDLTGLTELLDLTGKTGASFHMAHIVSTGLGRVPAFLQLVDQARARGLDVTTEV
                     YPYTAGSTGINSGIFDHDWQSKLGISYRDIEWPPTGQRFTGKEMWDEYRAKYREGVVI
                     IHAMREEWVEEALKHPGVMIASDGMPISSLDERAHPRGMGTFARVLGRYSREKKIITL
                     PDAISRMTLMPAQRFEKMSPAMRRKGRLSVGADADITVFDADQVIDRATFAEPNQFSK
                     GIIHVLVGGTVVVRDGRMVEGVFAGKPVRATMKTGG"
     gene            2845..>3900
                     /locus_tag="VNU03_04800"
     CDS             2845..>3900
                     /locus_tag="VNU03_04800"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP73805.1"
                     /translation="MLSTYDAIVIGAGISGMYQLHRLRGLGLRVRVFEAGTGVGGTWY
                     WNRYPGARFDSESWTYGYSFSDEILREWEWSEHFAAQPETLRYCNFVADKLDLRRDIE
                     FNCRIRTAVYDEAANQWEIETVDGRRARARFLITAIGPLSTPTMPTIPGVETFRGEAY
                     HTGRWPHEPVSFAGKRVGVIGTGATAVQAITEIAKTVGHLTVFQRTPNWCAPLHNSKI
                     DAATQARIKATYPEIFALCRDSFGCFIHQADPRKAMEVSAEEREAFYEKLYREPGFGI
                     WMGNFRDVLVDADANATITEFMRRKIRARVRDPKVAEKLIPTNHGFGTRRVPLESGYY
                     EVYNQPNVRLIDIRETPI"
BASE COUNT          665 a         1314 c         1342 g          579 t
ORIGIN      
        1 cccgtcacgc cggcgcatct gcagctcgcc gttgatcgcg gtgccctccg ccgcgactcg
       61 ctccaggagc tggtcgcgct ccttcggctg ggcgtagagc gtcccgacgt tcgttcccag
      121 cacgtcggcc gggctctcga agccgaggat ccgggcgaag gccgggttgc agtccacgat
      181 gcgcccgtcc gcgagggtga gggacatgcc ggccgggttg cgctcgaaca gcgtgcggta
      241 gcgccgctcc gattcctcca gcgccgcggt gcgcgtctgc aggagcgcca tcgcgtcgcg
      301 gagcgcgccc ttcacccccg agagctcgga gagggtgaag tcggggagct ccggcaccgt
      361 ctcgccgcgg ccgaaggcgc tgaacgcggc ggtgagctgc cgcaggcggc cggcgaaccg
      421 ccgcccggcc aggtgcgtga ggccgaggga gagcacgagc gcaccgaccg cggccgccga
      481 cagcagccag agcgaccggt ggagcgggcc ctcgagcgac tcgcgcggga ccgcgatgcc
      541 caccgtgaac cgggaccact tggcgcggct gaaggcggtg taggcggtcg ctccgtcgtg
      601 ggtggcgccg cccgtgaaca ccgcctcgtc ctgctcgcgg gcccgcgaca cgaagcccgc
      661 gctcgcgggc gtgcccaccc gctgctccgg gccctcggtg gtggcgacca ccacgccctt
      721 gcgatccacg atcgtcgcgc ccgcgacgtc ggcggcggcc agcaccgagg cgaagctctc
      781 cggcgtcatg accgcgacca ggatgtgatg gacctttccg gcccggagca aggggagcgt
      841 gaccgagatc acccagcggc cagccgcggg gccgatctcc agatcggaga cctgcggctc
      901 gccggtctgg agcgtgcgct ggaacgtctc gagcgcggcc acgctcggca gcgggctccc
      961 gagcgggcgc aggaggttga gcacctgccg gcccgacgga tccacgaggg ccaccgtcag
     1021 ccaccgtcga taggcgtcgc gcgcgagccg cgcctcctcg tagaagttcc ggaggtcgcc
     1081 cgactcgaga tcggtggagg tcgcgagtcc cttcagcccg gccagcgagg tctcgacctc
     1141 gcgatccacc gccacggtca gggagcgggc gtgatagagg agcgcctgct ccatgttgcg
     1201 acgctcgtcc tcgtgcgccc accagatcgc gaggccggcc acccccagca gagggacgag
     1261 ggtgaggccg acgacggcga ggagaaacgt ccgaagcttc atgggcgccg gatcggcaag
     1321 ctttctggta gccgcttcac tctgttattc tccggcatgt agtcaaggag aacgcatgaa
     1381 atatcgttcg ctcgtattgt tgtgcctcgt ggcggttctt ccgttgtcga cctcgatccg
     1441 gccggcccag tctcgcaccg ccacctttga tctcgtcctg accgggggcc gggtcatcga
     1501 cccggccagc tggctggacg gggtcaggaa tatcgggatc agcaatggca cggtggccgc
     1561 catctcgacg ggcccgctgt cgggaaagca gacgatcgat gtggcgaacc tcgtcgtcgc
     1621 gccgggcttc atcgacatgc acgcgcacgg acagaacgtg ccttcgcaga cctatcaggt
     1681 cagagacgga gtcaccaccg cgctcgagct cgagatcggc gcgtttcccc tgaacgctct
     1741 cggcgcgcgc gaaggacgag cgctgatcaa ctacggctat tcggccgggc acaccgccgc
     1801 gcgcatcacc gtcaagagag gcaaccggca ggccgcctat cacgaagcgc tgaccgagag
     1861 cgagctgaag gagatgatcg ggtacctcgc gacggcgctc gacgaaggcg cgctcggcat
     1921 cggcgtcggt ctcgactatg tctcgctcgg ggtcaaaccc gacgagctgg aagctctctt
     1981 tcgccttgcc gccgcgcgaa aggttcccat cttcgttcac attcgtatgc cggatgaccg
     2041 caacgacttg accggcctca ccgagcttct cgatctcacg ggaaaaaccg gggcgtcatt
     2101 tcacatggct cacatcgtga gcaccggcct cggccgcgtg cccgcattcc tccagttggt
     2161 cgatcaggcg cgggcccggg gtctcgacgt cacgaccgag gtgtatccgt acacggcggg
     2221 ctctaccggg atcaattcgg gcatcttcga tcacgactgg cagagcaagc tcgggattag
     2281 ctaccgcgac atcgagtggc cgccgaccgg acagcgattc accggcaagg agatgtggga
     2341 cgagtatcga gcgaaatacc gagagggcgt cgtcatcatt cacgcgatga gagaagaatg
     2401 ggtcgaggag gcgctcaagc atccgggggt gatgatcgcc tcggacggga tgccgatctc
     2461 gagcctcgac gagcgcgcgc accctcgagg catgggcacc ttcgcgcgcg tgctcggccg
     2521 ttactcgcgc gagaagaaga tcatcaccct cccggacgcc atcagccgga tgaccctgat
     2581 gcccgctcag agattcgaga agatgtcacc cgcgatgagg aggaagggcc ggctcagcgt
     2641 gggggcggac gccgacatca cggtattcga cgcggatcag gtcatcgatc gagcgacgtt
     2701 tgccgagccc aaccagttct cgaaaggcat cattcacgtg ctggtcggcg gaaccgtggt
     2761 cgtccgggac gggagaatgg tcgagggcgt cttcgcgggc aaaccggttc gggccacgat
     2821 gaagacggga gggtagggcg gatcatgctc tcgacatacg acgccatcgt catcggcgcc
     2881 gggatctccg gcatgtacca gctccaccgg ctgcgcgggc tgggcctgcg ggtgcgggtg
     2941 ttcgaggccg gcaccggggt gggcggtacc tggtactgga accgctaccc cggagcgcgg
     3001 ttcgactccg agagctggac ctacggctac tccttctccg acgagatcct gcgcgagtgg
     3061 gagtggagcg agcacttcgc ggcccagccg gagacgctgc gctactgcaa cttcgtcgcc
     3121 gacaagctcg acctccggcg cgacatcgag ttcaactgcc ggatcaggac cgccgtctac
     3181 gacgaggcgg ccaaccagtg ggagatcgag accgtggacg gccggcgggc gcgggcccgg
     3241 ttcctgatca ccgcgatcgg gcccctgtcg acgccgacca tgccgaccat ccccggcgtc
     3301 gagaccttcc gcggcgaggc ctaccacacc ggccggtggc cgcacgagcc ggtgagcttc
     3361 gcgggcaagc gggtgggggt gatcggcacc ggggcgaccg cggtgcaggc gatcaccgag
     3421 atcgccaaga cggtcgggca cctcacggtg ttccagcgca ccccgaactg gtgcgcgccg
     3481 ctccacaaca gcaagatcga cgcggccacc caggcccgga tcaaggcgac ctatccggag
     3541 atcttcgcgc tctgccgcga ctcgttcggc tgcttcatcc accaggccga tccacgcaag
     3601 gccatggagg tgagcgcgga ggagcgtgag gcgttctacg agaagctcta ccgcgagccc
     3661 ggcttcggga tctggatggg caacttccgc gacgtgctgg tcgacgcgga cgccaacgcc
     3721 accatcaccg agttcatgcg ccggaagatc cgcgcccggg tccgggaccc gaaggtggcg
     3781 gagaagctga tcccgaccaa ccacggcttc gggacccggc gcgtgccgct cgagagcggt
     3841 tactacgagg tttacaacca gccgaacgtg cgcctgatcg acatccgcga gacgccgatc
//
LOCUS       DATMCV010000208         8129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_10894, whole genome shotgun sequence.
ACCESSION   DATMCV010000208 DATMCV010000000
VERSION     DATMCV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10894"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..760)
                     /locus_tag="VNU03_04805"
     CDS             complement(8..760)
                     /locus_tag="VNU03_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73806.1"
                     /translation="MLTVEGLSKRFGGFTAVNHVSFSVERGEIVGLIGPNGSGKSTIF
                     NLLSGALTPNAGSIRFEGHELAGQPPHRIINLGIGRTFQIPRPFRRLSLLENVALAGF
                     FGQGRASRRRAFEAAERALGLVGLPADPGASVDGLGAAALKKLELAKALATDPKLLLA
                     DESLSGLDEQEMDQAALMLRRICTDLGITIIWVEHIMGVLMRVVDRVMVLDHGEKIAE
                     GLPAAVAADPRVIEVYLGTQSGAARAVASARS"
     gene            complement(794..2056)
                     /locus_tag="VNU03_04810"
     CDS             complement(794..2056)
                     /locus_tag="VNU03_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73807.1"
                     /translation="MSTPPIRDAHAQAKKLKIGVVFDYTGPLAGGGSDLHALGAKIMI
                     DHFAKKGGVEGYQIEAIYADAQSKPEIAINEATRLIEQEKVDMLLGFYSSAQCVPAAA
                     RVEQLHKFMWITTCISSAVLENRNLKYVFRIQPSGRQFGLMSTDFVAQNAQAKLGKDP
                     KGLRVAIIHEDGAYGVDVSKGNEEGAKKAGFNVVLKEGYSATAPDLAPLVTKLKRARP
                     DVIFHTGYNPDISLFLRQARELGLRFNALIGHGAGYGVYDKLKEALGKDVNYFYNVDP
                     ISIWLANDKALKPELLPLIKMVGEEYEKARPGTTVRSAHVGMAASNTYVLMTEVLPRA
                     IKKYGGIDADSLRKAALEVNLPEGGTMLGFGVKFAGEGSPMAGQNERAFPVIVQYVDD
                     KPYVVWPKSLQLREPVLKLPSSSPYAAE"
     gene            complement(2220..3557)
                     /locus_tag="VNU03_04815"
     CDS             complement(2220..3557)
                     /locus_tag="VNU03_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP73808.1"
                     /translation="MADPDPQSIDRRTFVKTAAVAAAAAGGIEGILAARRAPAFAQGT
                     KLHWVRWVDFVPESDVELKRQMPEASKALGAEVTLETINANDLQARITAAIQSGSGAD
                     IFLMQYNWPQLYANALVDVSDVANDLAKAEGGFYEIYPPSCNVNGKWLSVPHSVLGNA
                     VAYRKSWLKEAGASEYPKTWDDARKLFAGLKKKGKPYGQTLGHTFGDAPVFTYTMTWA
                     FGAAETDKSGKKVVLDSKGAVEAVKFMQAFWKECCDEGGLAWDDTNNNRAFHAGEVSA
                     TLNGASIYIVAKRQKDKIKDDKGEPMWTDIDHAPLPAGPAGAYLLFLNHSHAVMKYSK
                     NQKLAKDFLRWLHRKENYEKWFLSQEAYSVGATKAWENHPMWANVDGPLKMFRTAARN
                     TRLFGYGGPSTAKSTEVFTKYIITDMYAKGVQGMKAEDAVKWAEGELKKIYES"
     gene            complement(3625..4521)
                     /locus_tag="VNU03_04820"
     CDS             complement(3625..4521)
                     /locus_tag="VNU03_04820"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWP73809.1"
                     /translation="MAEHAHLHGIVPPMTTPFRTDDTIDEGALRAETRYLIDTARVHG
                     LAVTGSTGEGHALTTDEVRGIAAAVVDEARGRVPVIAGIITDSTASAVERGLAVRDLG
                     VAALQVTPVHYLFRPDDDAMVRHFDTLARRTGLPIIIYNVVPWTYLSPQLLARIIDAV
                     PGVIGVKQSAGDMKLLADLLLLVGDRARIMSAVDALLYPSFALGAHGAIAAILTAVPT
                     LCVQLWDAVQAGDHKQALALHEKLLPIWNAISHDNLPANVKHCQELMGRPAGVPRAPM
                     PATSAAQADPIRRALHGAGVLP"
     gene            complement(4670..6385)
                     /locus_tag="VNU03_04825"
     CDS             complement(4670..6385)
                     /locus_tag="VNU03_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015717169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HWP73810.1"
                     /translation="MRIYYDIRLSDRSITKRELTGEAIVKAGRYFIAKTLLELGAATV
                     DPLSPANPLIFSAGPFAGTSFSNANRTSVGCKSPLTGGVKEANGGGSFSYGLGQQHIA
                     GFTLLDASPDWVVIHFKKDGTIEFDDAGPYLGKGNFEAQAMLHARYGKKVTIALCGPV
                     GEYQGLIAGIAFSDKDGRPARLSARGGVGAVMGAKKVKAVVVDLDKIPSFSEPKKVNA
                     AIKNYAKMLQADSMVSNFYAKIGTMGMADLQNTLGGLPVRNFSAGQQVDVSKGEKFKM
                     GGEYISQLNVSRGGEHTHACMPGCVIQCSNIYADKDGKEVVSPVEYETLGLLGTNCGL
                     SDPDDLAQMNYIANDLGVDTIETGAMIAVLMEAGLGAFGDLRFMADCFAEIRNGSEKG
                     RIWAQGTARVGEHYGVKRVPVIKKQAISAYDPRVVEATGITMMATAQGADHTAGNLPR
                     LKTREMETLAIVEQSVAHQARVAANDSLGLCIFGMSVTNPNTEWLTDTINAAHGTSLT
                     KEFFEALGRQTLKLEHEFNKRAGFTAKDDELPAFFYAEALPPTNHVARFHAADVQGMY
                     ERLPA"
     gene            complement(6403..7245)
                     /locus_tag="VNU03_04830"
     CDS             complement(6403..7245)
                     /locus_tag="VNU03_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HWP73811.1"
                     /translation="MTYCVGIWTRDGLVMASDSRTNAGHDQVNIARKMHTFVQPGERV
                     FILLSSGSLSCTQSIITLLKRDFDQGKGLAQAPSLYDAARVIGEQVRRVSDMDRDALE
                     RDDFKFNVNLVLGGQVRGESPGLFIIYPQGNPLQASEDSPYLQIGETKYGRPILDRGI
                     RFHRTTLEEAAKYALLSLDSTMRSNVTVGPPIDLLAYGVDEFEITRLRRFAASDPDLI
                     KIGMRWEQALRQAVGRLPQIRFRARRPAEPSQLPAAETIELVEPGVSEPAGELQPQAK
                     PPSS"
     gene            7354..>8129
                     /locus_tag="VNU03_04835"
     CDS             7354..>8129
                     /locus_tag="VNU03_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWP73812.1"
                     /translation="MRIRIGHELTFEIPRPTPMLLMLYVHPDTVPALRQPEAIQTEPV
                     GDVESFVDWFGNRAARIVAPPGTLRLRYDNVVEDSGRPEPTIEGQPLRPVPELPPEAW
                     RYLLASRYCEVDRMSAIAWDLFGKTPETWERVQAVVDWVHRNVTFGYQFSRPTKTAFD
                     VFTERQGVCRDFQHLTITFLRALNIPARYATGYLGDIGVPKDPNPMDFSAWLEVYLGD
                     RWHTIDARHNEPRIGRVLMGRGRDAVDVALTTSFGSTTLTG"
BASE COUNT         1146 a         2774 c         2847 g         1362 t
ORIGIN      
        1 caccggatca gctccgcgcg gaggcgaccg cgcgggccgc ccccgactgg gtcccgaggt
       61 acacctcgat cacccgggga tcggccgcga cggcggcggg caggccctcc gcgatcttct
      121 cgccgtggtc cagcaccatc acccggtcca ccacccgcat cagcacgccc atgatgtgct
      181 cgacccagat gatcgtgata ccgaggtcgg tgcagatgcg gcgcagcatc agggccgcct
      241 ggtccatctc ctgctcgtcg agcccgctca ggctctcgtc ggccaggagc agcttggggt
      301 cggtggcgag ggccttggcc agctcgagct tcttgagcgc ggcggccccg agcccgtcca
      361 cggaggcccc ggggtcggcg ggcaggccca ccaggcccag ggcccgctcg gcggcctcga
      421 aggcccggcg acgcgaagcc cgcccctggc cgaagaagcc ggcgagggcc acgttctcga
      481 ggagggagag ccggcgaaac gggcgcggga tctggaaggt gcggccgatg cccaggttga
      541 tgatccggtg cggcggctgc cccgcgagct cgtggccctc gaagcggatg gagcccgcgt
      601 tgggcgtgag cgcccccgag agcaggttga agatcgtgct cttgcccgag ccgttgggcc
      661 cgatcagccc gacgatctcg ccccgctcga ccgagaacga gacgtggttc acggcggtga
      721 agccgccgaa gcgcttggac agcccctcga cggtcagcac ggagcgatca cgcgatcgcg
      781 cggccccgcc gcgtcactcc gccgcgtagg gcgaggagga cgggagcttc agcaccggct
      841 cgcggagctg gaggctcttg ggccagacca cgtacggctt gtcgtccacg tactgcacga
      901 tcaccgggaa ggcgcgctcg ttctgcccgg ccatgggcga gccctcgccc gcgaacttga
      961 ccccgaaccc gagcatggtg ccgccctcgg gcaggttgac ctcgagcgcg gccttgcgga
     1021 gcgagtcggc gtcgatcccg ccgtacttct tgatggcccg cggcagcacc tcggtcatga
     1081 gcacgtaggt gttgctcgcc gccatcccca cgtgggccga gcggaccgtg gtccccggcc
     1141 gggccttctc gtactcctcg cccaccatct tgatcagggg cagcagctcg ggcttgaggg
     1201 ccttgtcgtt ggcgagccag atcgagatcg ggtcgacgtt gtagaagtag ttgacgtcct
     1261 tgccgagcgc ctccttcagc ttgtcgtaga ccccgtagcc ggccccgtgg ccgatcaggg
     1321 cgttgaaccg gaggccgagc tcacgcgcct gccgcaggaa gagcgagatg tccgggttgt
     1381 acccggtgtg gaagatgacg tccggccggg cgcgcttgag cttcgtcacg agcggggcca
     1441 gatcgggcgc ggtggcggag tagccctcct tcaggaccac gttgaagccc gccttcttgg
     1501 ccccctcctc gttgcccttc gagacgtcga ccccgtaggc gccgtcctcg tggatgatcg
     1561 cgacccgcag gcccttggga tcctttccga gcttggcctg ggcgttctgg gccacgaagt
     1621 cggtcgacat caggccgaac tgccgcccgc tcggctggat ccggaacacg tacttgaggt
     1681 tgcgattctc gagcaccgcg gaggagatgc aggtggtgat ccacatgaac ttgtggagct
     1741 gctcgacccg ggccgccgcg ggcacgcact gggcggagga gtagaagccg agcagcatgt
     1801 cgaccttctc ctgctcgatc aagcgcgtcg cctcgttgat cgcgatctcc ggcttggact
     1861 gggcgtcggc gtagatcgcc tcgatctggt agccctccac cccgcccttc ttggcgaagt
     1921 gatcgatcat gatcttggcc ccgagcgcgt gcaggtcgga gccgcccccg gccagcgggc
     1981 cggtgtagtc gaacaccacc ccgatcttga gcttcttggc ctgggcgtgc gcgtcgcgga
     2041 tcggcggggt cgacatcagc gcgagacccg cgatcacccc ggcggcgacg agggtccatg
     2101 cgatggatcg gcgcagtgac atgagcgtcc tcccaagcga cgtcggtcgg gaaacggagc
     2161 gtggtgcgcg gattacaagc tggcgggccc ttggttgtca agggcccgcc cgcgatgact
     2221 cagctctcgt agatcttctt cagctcaccc tccgcccact tcaccgcgtc ctccgccttc
     2281 atgccctgga cgcccttggc gtacatgtcg gtgatgatgt acttggtgaa gacctcggtc
     2341 gacttggcgg tcgacgggcc cccgtaaccg aagagccgcg tgttgcgcgc ggcggtgcgg
     2401 aacatcttga ggggcccgtc cacgttcgcc cacatgggat ggttctccca cgccttggtg
     2461 gccccgacgc tgtacgcctc ctggctgagg aaccacttct cgtagttctc cttccggtgg
     2521 agccagcgca ggaagtcctt ggccagcttc tggttcttgc tgtacttcat gaccgcgtgg
     2581 gagtggttca ggaagaggag gtaggccccg gcgggcccgg ccgggagcgg cgcgtggtcg
     2641 atgtcggtcc acatcggctc gcccttgtcg tccttgatct tgtccttctg ccgcttggcc
     2701 acgatgtaga tgctggcccc gttgagggtg gcggagacct ccccggcgtg gaaggcgcgg
     2761 ttgttgttgg tgtcgtccca cgcgagcccg ccctcgtcgc agcactcctt ccagaaggcc
     2821 tgcatgaact tgaccgcctc caccgccccc ttggagtcga gcaccacctt cttgccgctc
     2881 ttgtcggtct cggccgcccc gaaggcccac gtcatggtgt aggtgaacac cggggcatcc
     2941 ccgaaggtgt gccccagggt ctgcccgtag ggcttgccct tcttcttgag cccggcgaag
     3001 agcttgcgcg cgtcgtccca ggtcttgggg tactcgctcg ccccggcctc cttgagccac
     3061 gacttccggt acgccaccgc gttgccgagc accgagtggg gcaccgagag ccacttgccg
     3121 ttgacgttgc acgacggcgg gtagatctcg tagaagccgc cctcggcctt cgcgaggtcg
     3181 ttcgcgacgt cgctgacgtc cacgagcgcg ttggcgtaga gctgcggcca gttgtactgc
     3241 atcaggaaga tgtcggctcc cgagccggac tggatggcgg cggtgatgcg ggcctgcaga
     3301 tcgttggcgt tgatggtctc gagggtcacc tccgccccca gcgccttgga ggcctccggc
     3361 atctggcgct tcagctcgac gtcggactcc ggcacgaagt ccacccagcg cacccagtgc
     3421 agcttggtgc cctgcgcgaa cgcgggggcc cggcgcgcgg ccaggatgcc ctcgatgccc
     3481 ccggccgctg cggcagcgac ggcggcggtc ttcacgaacg tccggcgatc gatggactgc
     3541 gggtcgggat cggccatggt cggggtcctc cttgacccta acggtgaaga aacggacggt
     3601 gaggcggcgt tcgcgcccgg cgcgctaggg cagcacgccg gctccgtgca gcgcccgccg
     3661 gatcgggtcg gcctgggccg ccgaggtggc gggcatcggg gcccgcggca cccccgccgg
     3721 ccggcccatc agctcctggc agtgcttgac gttcgcgggc aggttgtcgt gggagatcgc
     3781 gttccagatc gggagcagct tctcgtgcag ggcgagcgcc tgcttgtggt cgccggcctg
     3841 caccgcgtcc cagagctgca cgcacagggt gggcaccgcg gtgaggatcg cggcgatggc
     3901 cccgtgggcg ccgagggcga acgacgggta gagcagcgcg tccaccgcgc tcatgatccg
     3961 ggcccggtcg ccgaccagga gcagcaggtc ggcgagcagc ttcatgtccc ccgcgctctg
     4021 cttgaccccg atcacgccgg gcaccgcgtc gatgatgcgg gccaggagct gcggagacag
     4081 gtaggtccac ggcaccacgt tgtagatgat gatgggcagc ccggtccggc gggccagggt
     4141 gtcgaagtgc cgcaccatcg cgtcgtcgtc cggacggaac aggtagtgca ccggcgtcac
     4201 ctgcagcgcg gccacgccca ggtcgcggac cgcgagaccg cgctccaccg cggaggcggt
     4261 cgagtcggtg atgatgccgg cgatgaccgg cacccgcccg cgcgcctcgt cgaccaccgc
     4321 cgcggcgatc ccgcgcacct cgtcggtggt gagcgcgtgc ccctcgccgg tgctgccggt
     4381 gaccgcgagg ccgtgcaccc gggcggtgtc gatcaggtag cgcgtctccg cccggagggc
     4441 gccctcgtcg atggtatcgt ccgtgcggaa cggggtggtc atcggaggaa cgatgccgtg
     4501 gaggtgcgcg tgctcagcca tcgcctggtc tcctgagccc ggaccgcggg cgcgttggcc
     4561 gagaccctaa gccgggatcc cgcggctgtc aaggcgccgg gacacgaggg ccccgccgtc
     4621 cgccccggag ggaagacggc ggggcccggg aaaccggagc cgcgccgggt caggcgggca
     4681 ggcgctcgta cattccctgc acgtcggcgg cgtggaagcg cgccacgtgg ttggtcgggg
     4741 ggagcgcctc cgcgtagaag aacgcgggca gctcgtcgtc cttcgcggtg aagccggccc
     4801 gcttgttgaa ctcgtgctcg agcttgaggg tctggcggcc gagggcctcg aagaactcct
     4861 tggtgaggct cgtgccgtgg gccgcgttga tcgtgtcggt gagccactcg gtgttgggat
     4921 tggtcacgct catcccgaag atgcagagac cgagggagtc gttggcggcc acccgcgcct
     4981 gatgggccac gctctgctcc acgatggcga gcgtctccat ctcgcgagtc ttgaggcgcg
     5041 gcaggttgcc cgcggtgtgg tcggccccct gggcggtggc catcatggtg atgccggtgg
     5101 cctcgaccac ccgcggatcg taggcgctga tcgcctgctt cttgatgacc ggcacgcgct
     5161 tcacgccgta gtgctcgccc acccgggcgg taccctgggc ccagatgcgc cccttctcgg
     5221 agccgttccg gatctccgcg aagcagtcgg ccatgaagcg caggtcgccg aaggccccga
     5281 gcccggcctc catcagcacc gcgatcatgg ccccggtctc gatggtgtcc acgcccaggt
     5341 cgttggcgat gtagttcatc tgggcgaggt cgtccggatc ggagagcccg cagttggtgc
     5401 cgagcagccc gagggtctcg tactcgaccg gcgagaccac ctccttgccg tccttgtccg
     5461 cgtagatgtt gctgcactgg atcacgcagc cgggcatgca cgcgtgggtg tgctcgccgc
     5521 cgcgggagac gttgagctgg ctgatgtact cgccgcccat cttgaacttc tcgcccttgg
     5581 agacgtccac ctgctgaccc gcgctgaagt tccgcaccgg caggccgccc agggtgttct
     5641 gcagatctgc catgcccatg gtgccgatct tggcgtagaa gttcgacacc atcgagtccg
     5701 cctgcagcat cttggcgtag ttcttgatgg ccgcgttcac cttcttcggc tcggagaacg
     5761 acgggatctt gtcgaggtcc accacgaccg ccttcacctt cttggcgccc atgaccgcac
     5821 ccactccccc gcgcgcggag aggcgggcgg ggcggccgtc cttgtcgctg aacgcgatgc
     5881 ccgcgatgag cccctggtac tcgcccaccg ggccgcagag cgcgatcgtg accttcttgc
     5941 cgtacctggc gtgcagcatg gcctgggcct cgaagttgcc cttgccgagg tagggcccgg
     6001 cgtcgtcgaa ctcgatggtg ccgtccttct tgaagtggat caccacccag tccggggagg
     6061 cgtcgagcag ggtgaagccc gcgatgtgct gctggccgag cccgtagctg aagctgccgc
     6121 ccccgttggc ctccttgacg ccgccggtca gcgggctctt gcagcccacg ctggtgcggt
     6181 tggcgttgga gaagctcgtg cccgcgaagg gccccgccga gaagatgagc gggttggccg
     6241 gggagagcgg gtcgacagta gccgcaccca gctcgagcag ggtcttggcg atgaagtagc
     6301 ggccggcctt cacgatcgcc tcgccggtga gctcgcgctt cgtgatgctc cggtcgctca
     6361 ggcggatgtc gtagtagatg cgcatcgctc gctcctcgct ggtcaggatg atggaggctt
     6421 ggcttgtggc tgcagctcgc ccgccggctc ggacacgccc ggctccacca gctcgatcgt
     6481 ctcggcggcc gggagctggg acggctccgc agggcggcgg gcccggaagc ggatctgggg
     6541 cagccggccc accgcctggc ggagggcctg ctcccagcgc atgccgatct tgatgaggtc
     6601 cggatcgctc gcggcgaagc ggcgcagccg ggtgatctcg aactcgtcca ccccgtaggc
     6661 gagcagatcg atcggcggtc ccaccgtcac gttcgaccgc atggtcgagt cgagcgagag
     6721 cagcgcgtac ttcgccgcct cttccagggt ggtgcggtgg aagcggatgc cgcggtccag
     6781 gatggggcgg ccgtacttgg tctcgccgat ctggagatac ggcgagtcct cgctggcctg
     6841 cagcgggttg ccctgggggt agatgatgaa gaggccgggc gactccccgc gcacctgccc
     6901 gcccagcacg agattgacgt tgaacttgaa gtcgtcgcgc tcgagcgcgt cgcggtccat
     6961 gtcggacacg cgccgcacct gctcgccgat cacccgggcc gcgtcgtaga gcgacggggc
     7021 ctgggccagg cccttgccct ggtcgaagtc gcgcttgagc agggtgatga tcgactgggt
     7081 gcacgagagg ctgccgctgc tgagcaggat gaacacccgc tcgcccggct ggacgaaggt
     7141 gtgcatcttg cgcgcgatgt tgacctggtc gtgccccgcg ttggtgcggg agtcggacgc
     7201 catcacgagg ccgtcgcggg tccagatgcc gacgcaatag gtcatggcgc gcccgtcggt
     7261 gagcctgccc ggttcctgag catcgtcgag tatcgtacca gaaccgccgc ggatgtgatt
     7321 gaccgacacc accggggcgg ataccgtgac cccatgcgga tccgcatcgg ccacgagctg
     7381 accttcgaga tcccgcgccc cacgccgatg ctcctcatgc tgtacgtgca cccggacacc
     7441 gtcccggccc tgcggcagcc ggaggccatc cagaccgagc cggtcggcga cgtcgagagc
     7501 ttcgtggact ggttcggcaa tcgcgccgcc cgcatcgtgg cgccgccggg cacgctccgc
     7561 ctgcgctacg acaacgtggt ggaggactcg ggccggcccg agccgaccat cgaaggacag
     7621 ccgctccggc cggtgccgga gctgcctccc gaggcctggc ggtacctgct cgcgagccgg
     7681 tactgcgagg tcgaccgcat gtcggccatc gcctgggatc tcttcggcaa gacgcccgag
     7741 acgtgggagc gcgtgcaggc ggtggtggac tgggtgcacc ggaacgtgac cttcggctac
     7801 cagttctcgc gcccgaccaa gaccgcgttc gacgtcttca ccgagcggca gggcgtgtgt
     7861 cgcgacttcc agcacctgac catcacgttc ctgcgcgcgc tgaacatccc ggcccgctac
     7921 gcgaccggct acctggggga catcggcgtg ccgaaggatc cgaacccgat ggacttctcg
     7981 gcctggctcg aggtctacct gggcgatcgg tggcacacca tcgacgcccg ccacaacgag
     8041 ccgcgcatcg gccgcgtgct gatgggccgc ggccgcgacg cggtcgatgt ggccctcacc
     8101 acctcgttcg gcagcactac cctcaccgg
//
LOCUS       DATMCV010000209        11494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_745838, whole genome shotgun sequence.
ACCESSION   DATMCV010000209 DATMCV010000000
VERSION     DATMCV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11494
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745838"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VNU03_04840"
     CDS             complement(<1..185)
                     /locus_tag="VNU03_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73813.1"
                     /translation="MPNSTRPPVGGGPEAVEASAEAGGVVGVGAAPAGGAAAAEGAAG
                     AGVAGTAGAEVGPGAAAL"
     gene            254..577
                     /locus_tag="VNU03_04845"
     CDS             254..577
                     /locus_tag="VNU03_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWP73814.1"
                     /translation="MAHYVMLSTLSESGRKVIHARPGWIRKVNQELSRRGAKVLAQYA
                     VLGPYDFVTILEAPDNETVTSISIEMGARGSVQMMTMPAIALDTFINRLERSRPTPKR
                     KGKRR"
     gene            585..1058
                     /locus_tag="VNU03_04850"
     CDS             585..1058
                     /locus_tag="VNU03_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWP73815.1"
                     /translation="MKVLALHPEKCTGCLRCELACSYMQTGQFQPSKSVIRVSPFEGY
                     TSYAPYTCTQCAEGWCMTSCPVGAIQITAVGAKDVVDDMCVGCKLCTIACPYGTMFYD
                     GDTQKAYKCNLCGGAPACASACPTEAITFEEAENADWIGDFAAERTARVLAMEAR"
     gene            1058..2410
                     /locus_tag="VNU03_04855"
     CDS             1058..2410
                     /locus_tag="VNU03_04855"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP73816.1"
                     /translation="MPARHLIIGGGTAGMNAIRTIREEERMRALDPSEITLVSAERPY
                     SRMVLPYYLWGTISESHVYTAPPAALTAWKVKALVGRKAKALDPAARTLTLDDDAKVE
                     FDDCLIATGSRAAKAPIPGADGPGVHCFWTLDEAKSVIAGMPPGSRVVMVGAGFIAFT
                     ILNAILARGAKLTIVEVAPRILPRMVDDACAGIVEGWLEDHGVAVRTGATVTRIEPVG
                     GKRKLSFKKGDPLTADLVIMATGIRTNLEWLQGSGIETAAEAPGGILVDDHLRSSAKT
                     IYAAGDVARGRNLITGLAEVHAIEPTAQEHGRVVGANMAGRDVAYRGSLLMNIVEVAH
                     LDVASFGQWEDPKAEVYTALRAGRSEYRKLLFHGGRLTGAVICGPAEAVWTTNDVGML
                     KGLVSSGVDLSTWKAHLKANPFDIKPAFIALHTTSRLLPETILGRPSQSPQLPQTVAL
                     "
     gene            2407..4320
                     /locus_tag="VNU03_04860"
     CDS             2407..4320
                     /locus_tag="VNU03_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HWP73817.1"
                     /translation="MSPTATTTRAAAKAAATPTYAYAGKLLRVNLSTGKCWTEAWSEK
                     DRREYLGGIGLGAKILYEEVGPKVQWDHPDNRFIMATGPLAGLPVWGTGGLTVITRGA
                     LTGGATSTQANGFFGANLKYSGYDAIVMQGQAKKLSYLYINDDVVEVRDAAHLAGKDT
                     WETQQALETEHGLAGHRLSVYAIGPAGENLVRFAAIHGDYGHVASKNGCGAVMGKKKL
                     KAVCIVRGTKALAAHDTRGLIQAADDIAHDLKTDPSTSTLYNLGTLPGVVNLSRLGAL
                     PIKNYTTNLTGDVDMKEWEGRKLREGFDHRGHQCNACGMRHCHIQVIPSGPHAGDRVD
                     EPEYEGWSGAGWTIGLTDKIPVSWLNTQIDRACVDVNEFGWVCGWVMECMEKGYLTEK
                     QVGFRLTWGDVEGANKLLQMISHRQGFGDILAEGVKRASEKIGGKAAECAIYTMKGAS
                     PRGHDHRGRWEEMLDTCTSSNGTMESANPTFQTEVGAPGRINPFDGEQVAKLVGMIRG
                     RRHFEDSLGGCSFTFRTRMANLASALSAATGWDFRLEDAVRFGNRTAAILRAFNLRCG
                     IGTEVEYPSARYGSQPVDGPAKEHDVRKQWDRMLDVWYETVNYDRKTGKPNRDLLRSL
                     DLPWLEKDLYGKK"
     gene            4395..4664
                     /locus_tag="VNU03_04865"
     CDS             4395..4664
                     /locus_tag="VNU03_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73818.1"
                     /translation="MTKQVGGDGTGSRLFDEDIRLGDTPRTVLRRFTARFPELDSALW
                     SSDHAELGSHIEVLVNDEVLGVTYELDTPLIGGERITLLGQFMGG"
     gene            complement(4858..6024)
                     /locus_tag="VNU03_04870"
     CDS             complement(4858..6024)
                     /locus_tag="VNU03_04870"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HWP73819.1"
                     /translation="MSTDVSALMKITDRPPVVMVEGHGSWLRDEEGKTYLDFVQGWAV
                     NCLGHSPAVIAETIAFQARRLINCSPAYYNAEMAQLATRLAEASGLAQVFFCNSGAEA
                     NEGAIKLARKWGARYRDGAHEIITMDHGFHGRTLATMAASGKAAWEPLFEPKVPGFTK
                     VTLGDLEAVKRAITPRTVAVMLEPIQGEAGVYPAGDEFLRELRAVTTEAGLLLILDEI
                     QTGIGRTGRFLAYEHAGIEPDILTLGKGLGGGVPLAALVAAEAVCCFEPGDQGGTFNG
                     SPLMTAIGNAVVEAVNQPAFLADVTAKGDYLTQRLLDLSARLGHGEVRGRGLLLALEL
                     RGTDAGKVSRTAMDRGLLVNAPRPDALRFMPALNVTREEIDRMLVLLEAVLRDS"
     gene            complement(6021..6518)
                     /locus_tag="VNU03_04875"
     CDS             complement(6021..6518)
                     /locus_tag="VNU03_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011397770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP73820.1"
                     /translation="MIRPPIHERQDGDILVSTDETRLDLDTVHGFLAGSYWAAGIPRE
                     VVERSIRHSICFGAFDRGGQVGFARVISDRATFAYVADVFVLDSHRGRGIGKQIMAAV
                     TSHPELQNLRLWTLFTRDAHGLYRQSGFREARYPDRLMERRQPQPYRVGAGNGANRAK
                     GEHTA"
     gene            complement(6531..7052)
                     /locus_tag="VNU03_04880"
     CDS             complement(6531..7052)
                     /locus_tag="VNU03_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HWP73821.1"
                     /translation="MKNIYADGSGRGGAGGGEGGRRRRAILAVVGEGGVRSQEDLQRR
                     LRRQGIVVAQPTLSRDLKALGLAKAPTGYVTTPAPAFVPTEARQGALDRALGEFVLSV
                     RAAASLVVIRTPPAGAHPLARALDETELSDVIGTIAGDDTVFVATPGPSAALRLERRL
                     RAPLAPARVGRRA"
     gene            7109..7681
                     /locus_tag="VNU03_04885"
     CDS             7109..7681
                     /locus_tag="VNU03_04885"
                     /inference="COORDINATES: protein motif:HMM:NF022607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HWP73822.1"
                     /translation="MSTRAWWVGVPAALLGLIALAAVYATTTPRYDLYRLGAAVQRHD
                     VDDALRYFDVERIADRATDVLVADYLARQPAPATPAEANGRQLVASLAKRRLRPQVID
                     RVRVEVRRSVERAGAPAGAVVLPVGVVAVLKGFDVSRDGPDAWVSYHDPKEGPVRFRM
                     ARQPGEPWKISEFDPDWVRRRAREDPVRLR"
     gene            7774..8664
                     /locus_tag="VNU03_04890"
     CDS             7774..8664
                     /locus_tag="VNU03_04890"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HWP73823.1"
                     /translation="MAQVVGFIGLGIMGRPMAKNLLKAGYPLIVHSRSQGPVQELVGA
                     GAKAAATPRDVAAQCDVVIAMLPNSPDVELVALGKDGLIEGAKRGQLLADMSTISPLV
                     SQKVGKALAAKGVRMLDAPVSGGEKGAIDAALSIMVGGEKADFDAALPIFQALGKTIT
                     HLGPLGAGGFTKLANQIIVAVNLTALGEALTLARKAGLDRALTLKALGGGLAGSKCLE
                     QKTPNYVGGTYNPGFKIDLHFKDLGLIMESSRALGVPLPCTAVVQELFNAMRVKGKGG
                     LDHSGVITLLEELAGVPEKS"
     gene            8661..9695
                     /locus_tag="VNU03_04895"
     CDS             8661..9695
                     /locus_tag="VNU03_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP73824.1"
                     /translation="MRATVDRPGLDSQGRSPLIKIRRVRKGDLSKVRDVIEQAFGDFF
                     ERQVGTRPRQVFGGAQYVHHRWLMEPWGCFVAEEGDGKIVGAAIAALWGTVGVIGPVA
                     VLTNYQNQDIGQQLLTACQEFFDENKATLQGLATYPYSPKHLVLYQKFGYKPKGLVVV
                     TSKALDRPDSPAAAARPALKPGLAVRRYSTLEEARKKQVMQKVRRITNGFWRGLDLAK
                     EIEIVDGLTLGDTLLLEKGREVIGFAVCHLPPNSEAPHGSAYVKLLAIDSRHRKPEHL
                     QALLQGVEEMASAGQLQRVVVPAYTYYWTAYQTLLERGYHVDFTMVRMKRGKQEDYED
                     ASDLVLDDWR"
     gene            9703..10095
                     /locus_tag="VNU03_04900"
     CDS             9703..10095
                     /locus_tag="VNU03_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HWP73825.1"
                     /translation="MTRSSRPASLAALLLWVPLVLLMVSSASPHAIVLESSPVHDAVL
                     ERAPGQVTLRFNSKLEKRFTRITLATGDRPPAPVAVPGDDDASPPDRIVIPLSPLAPG
                     VYVLRYRVLAVDGHITEGALRFTVGPPR"
     gene            10097..>11494
                     /locus_tag="VNU03_04905"
     CDS             10097..>11494
                     /locus_tag="VNU03_04905"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWP73826.1"
                     /translation="MAQLAGFLDVLLRGFGTVGLAAAVGGLAYILLVLRPGPGAGGLG
                     GAARDRAVGLIEAGALLVAATTALLLLVVHPWALADETGRWPLRDFLTTHFGLAELGR
                     VTLALLLVAAVRGVRGGAATRGRWALALGLGLLLMANAAWLAHAASRLHEREPLMVVT
                     VLHQLGAVVWVGGLTHLVGFALLWRRAGGPDAEPLGVRVLARFSAVAIAALVLVLGPG
                     IVLTWSYVGAWGGLVGTGYGVMVLTKVALLACAMVLGGLNFLLLRGGGLLLRGGGGHR
                     GAAAVAARVPAFIEAEVGFGITLLLAAASLTSLPPSVDVVADRATPAEVAARFRPAMP
                     RLTSPPVAQLLAAAAPMEDTLAQRQPEEYAWSEYNHHAAGFFVFLMGVLALADRAGFV
                     RWARHWPLLFLGLAGFLFVRNDPRAWPLGPAGFWESMVLPDVLQHRLVVLLVVSLGLF
                     EWMVRSGRLTRPGGRL"
BASE COUNT         1672 a         4082 c         4103 g         1637 t
ORIGIN      
        1 agcgccgccg ctcccggccc gacttccgcg ccggcggttc cggcgacgcc ggcacccgcg
       61 gctccttcgg ccgccgccgc tccgcccgcc ggcgcggcgc cgacgcccac cacgccgccg
      121 gcctcggccg aggcctcgac cgcctcgggc ccgccgccca ccggcgggcg cgtggagttc
      181 ggcatcaccg gcgagagcga ctgagcgctt tgttatacta cgcgcaccgt ccgcagcttc
      241 caggaggtgc ctcgtggcgc actacgtgat gctgagcacg ctgagcgaat ccggccggaa
      301 ggtcatccac gcccgtccgg gctggatccg caaggtcaac caggagctgt cgcggcgcgg
      361 cgccaaggtg ctcgcgcagt acgcggtgct cggtccctac gacttcgtga cgatcctcga
      421 ggcgcccgac aacgagacgg tgacgtcgat ctccatcgag atgggcgcgc gcggctcggt
      481 gcagatgatg acgatgcccg cgatcgcgct cgacaccttc atcaaccgcc tcgagcgcag
      541 ccgccccacg ccgaagcgga aaggaaagcg gcgctgaccc cccgatgaaa gtcctcgcgc
      601 tccatcccga gaagtgcacc ggctgtctcc gctgcgagct cgcgtgctcc tacatgcaga
      661 ccgggcagtt ccagccgtcg aagtcggtga tccgggtctc tcccttcgag ggctacacct
      721 cctacgcgcc ctacacctgc acccagtgcg ccgagggctg gtgcatgacg tcctgcccgg
      781 tcggggcgat ccagatcacc gcggtgggcg cgaaggacgt cgtcgacgac atgtgcgtgg
      841 ggtgcaagct ctgtacgatc gcgtgcccct acgggacgat gttctacgac ggcgacaccc
      901 agaaggccta caagtgcaac ttgtgcggcg gcgccccggc ctgcgcgtcc gcgtgcccga
      961 ccgaggcgat caccttcgag gaagccgaga acgccgactg gatcggcgac ttcgcggccg
     1021 agcgcaccgc gcgggtgctc gcgatggagg cccgctgatg ccggcccgtc acctgatcat
     1081 cggcggcggc accgcgggca tgaacgcgat ccggacgatc cgggaagagg agcggatgcg
     1141 ggcactcgac ccgtccgaga tcacgctggt ctccgccgag cgcccctact cccggatggt
     1201 gctgccgtac tacctctggg gcaccatctc cgagagccat gtgtatactg cgccgcccgc
     1261 ggcgctgacc gcctggaagg tgaaggcctt ggtcggccgg aaggcgaagg ccctcgaccc
     1321 ggcggcccgg accctcaccc tggacgacga cgccaaggtg gagttcgacg actgcctgat
     1381 cgccaccggg tcgcgcgccg ccaaggcccc gatcccgggg gccgacggtc ctggggtcca
     1441 ctgcttctgg accctcgacg aggccaagag cgtgatcgcg ggcatgccgc cgggcagccg
     1501 ggtggtcatg gtcggcgcgg ggttcatcgc gttcaccatc ctcaacgcga tcctggcccg
     1561 cggggccaag ctgaccatcg tggaggtggc gccgcggatc ctgccgcgca tggtggacga
     1621 cgcgtgtgcc gggatcgtcg agggctggct cgaagaccac ggtgtggcgg tgcgcaccgg
     1681 cgcgaccgtg acccggatcg agccggtagg gggcaagcga aagctctcct tcaagaaggg
     1741 cgacccgctg accgccgacc tcgtgatcat ggccaccggc atccgcacca atctcgagtg
     1801 gctacagggc tcgggcatcg agacggcggc ggaggccccc ggcggcatcc tggtggacga
     1861 ccacctgcgc tcgagcgcga agacgatcta cgcggcgggc gacgtggcgc gcgggcgcaa
     1921 cctcatcacc ggcctggccg aggtgcacgc gatcgagccc accgcccagg agcacgggcg
     1981 cgtggtgggc gccaacatgg cgggccgcga cgtggcctat cgcggcagcc tgctcatgaa
     2041 catcgtcgag gtcgcgcacc tcgacgtggc ctcgttcggc cagtgggagg acccgaaggc
     2101 cgaggtctac accgcgctgc gcgccggccg ctcggagtac cgcaagctgc tcttccacgg
     2161 cgggcgcctc accggggcgg tcatctgcgg gcccgccgag gcggtgtgga ccaccaacga
     2221 cgtgggaatg ctgaagggtc tcgtgtcttc cggtgtcgat ctctcgacct ggaaggcaca
     2281 cctgaaggcg aacccgttcg acatcaagcc cgcgttcatc gcgctccaca cgacctcgcg
     2341 actgctgccc gagaccatcc tgggccggcc gtcgcaatca ccccagcttc cccagacggt
     2401 ggccctatga gcccgaccgc gaccacgacc cgcgccgccg cgaaggccgc ggcgacgccg
     2461 acctacgcct acgccggcaa gctcctccgg gtgaacctct ccaccggcaa gtgctggacc
     2521 gaagcgtgga gcgagaagga ccggcgcgag tacctcggcg gcatcggcct cggggccaag
     2581 atcctctacg aggaggtcgg tccgaaggtg cagtgggatc accccgacaa ccgcttcatc
     2641 atggccaccg ggcccctcgc gggcctgccg gtgtggggga cgggcgggct gaccgtcatc
     2701 acgcgcggcg cgctcaccgg cggcgccacg tccacgcagg ccaacggctt cttcggcgcc
     2761 aacctcaagt attcgggata cgacgcgatc gtcatgcagg gacaggccaa gaagctgtcg
     2821 tacctgtaca tcaacgacga cgtggtcgag gtgcgcgacg cggcccatct ggccggcaag
     2881 gacacctggg agacccagca ggcgctcgag accgagcacg gcctcgccgg ccaccggctc
     2941 tcggtctacg ccatcgggcc cgcgggcgag aacctggtgc gcttcgcggc catccacggc
     3001 gactacggcc acgtcgcctc caagaacggc tgcggcgcgg tgatgggcaa gaagaagctc
     3061 aaggcggtgt gcatcgtgcg cggcaccaag gccctcgccg cccacgacac ccgcgggctc
     3121 atccaggccg ccgatgacat cgcgcacgac ctcaagaccg acccgtcgac ctccacgctc
     3181 tacaacctgg gcacgctccc cggtgtggtg aacctctcgc gcctcggggc gctgccgatc
     3241 aagaactaca cgaccaatct caccggcgac gtggacatga aggagtggga gggccggaag
     3301 ctccgcgagg gcttcgatca ccgcgggcac cagtgcaacg cctgcggcat gcgccactgc
     3361 cacatccagg tgatcccgag cggtccacac gcgggtgacc gggtcgacga gccggagtac
     3421 gaggggtggt cgggcgcggg ctggaccatc ggcctcaccg ataagatccc ggtctcctgg
     3481 ctgaacacgc agatcgaccg ggcctgcgtg gacgtcaacg agttcggctg ggtctgcggc
     3541 tgggtgatgg agtgcatgga gaaggggtat ctcaccgaga agcaggtggg cttccgcctc
     3601 acctggggcg acgtcgaggg cgcgaacaag ctgctccaga tgatcagcca ccggcagggc
     3661 ttcggcgaca tcctggccga gggcgtcaag cgcgcctcgg agaagatcgg cggcaaggcg
     3721 gcggagtgcg cgatctacac catgaagggt gcgtctccca gaggccatga ccaccgtgga
     3781 cgatgggagg agatgctcga cacgtgcacg tcgtccaacg ggaccatgga gagcgccaac
     3841 ccgacgtttc agaccgaggt aggcgccccg ggtcgcatca atcccttcga cggcgagcag
     3901 gtggccaagc tggtcggcat gatccgcggc cggcgccact tcgaggactc cctggggggc
     3961 tgctcgttca ccttccgcac ccggatggcc aacctggcca gcgcgctctc cgccgcaacc
     4021 ggctgggact tccgcctcga ggacgcggtg cgcttcggca accgcaccgc cgcgatcctg
     4081 cgcgccttca acctgcgctg cggcatcggc accgaggtgg aatacccgtc cgcccggtac
     4141 ggctcgcagc cggtcgacgg ccccgcgaag gagcacgacg tccgcaagca gtgggaccgc
     4201 atgctcgacg tctggtacga gacggtcaac tacgaccgca agaccggcaa gcccaaccgc
     4261 gacctgctcc gctccctcga cctgccctgg ctcgagaagg acctctacgg aaagaagtag
     4321 agggggagag cgagccgttg ccgggcgagg cgcagccgaa ggcgagccga gcgcacatag
     4381 aagtcaccac gtgggtgacc aagcaagtcg gcggtgacgg caccggcagc cgactgttcg
     4441 acgaagacat ccgcctcggt gacacgcctc gcacggtgct ccgccgcttc accgcacggt
     4501 ttccggagct cgacagcgcg ctgtggagct ccgaccacgc cgagctgggc tcccacatcg
     4561 aagtcctggt gaacgacgag gtcctcggcg tgacctacga gctcgacacg ccgctgatag
     4621 gcggcgaacg catcacgctg ctcggccagt tcatgggcgg gtagacgggc ccggaggggc
     4681 agcggcggcc ggcgggggca gtgggggccg ggtaacccgt tccctccggc gctcgtgacg
     4741 ccgatgggta cccggattgg gaactcctga cgtatgagcc gaaggggcag cgggtcgtgg
     4801 ttagaggccc ccactgcccc cgccggccgc cgctgcccct ccgggcccgc cgccgcgcta
     4861 gctatcgcgc agcaccgcct ccagcagcac gagcatccgg tcgatctcct cgcgcgtcac
     4921 gttcaacgct ggcatgaagc ggagcgcgtc cggccgcggg gcattgacga gcagcccgcg
     4981 atccatggca gtccgcgaga ccttgccggc gtcggtgccg cgaagctcga gcgcgagcag
     5041 gagcccgcgc ccacgcacct cgccgtggcc gagcctcgcg gacagatcga ggaggcgctg
     5101 ggtcaggtaa tcgcccttgg cggtgacgtc ggccaggaac gccggctggt tcacggcctc
     5161 gaccaccgcg ttgccgatgg cggtcatgag gggagagccg ttgaaggtgc cgccctggtc
     5221 gccgggctcg aagcagcaca ccgcctcggc ggccacgagg gccgccaggg gcaccccgcc
     5281 tcccaggccc ttgccgagcg tgaggatgtc gggctcgatt cccgcgtgct cgtaggcgag
     5341 gaagcgtccg gtgcggccga tcccggtctg gatctcgtcg aggatcagca ggagcccggc
     5401 ctcggtggtg accgcgcgca gctcccgcag aaactcgtcc cccgcggggt agacgccggc
     5461 ctcgccctgg atgggctcga gcatgaccgc gaccgtccgc ggcgtgatcg cgcgcttcac
     5521 cgcctccagg tcgccgagcg tgaccttggt gaagcccggg accttgggct cgaagagggg
     5581 ctcccacgcc gccttgccgg aggcggccat ggtcgccagg gtgcggccgt ggaagccgtg
     5641 gtccatggtg atgatctcgt gcgcgccgtc gcgataccgc gcgccccatt tccgcgccag
     5701 cttgatcgcg ccctcgttgg cctcggcgcc gctgttgcag aagaagacct gggcgaggcc
     5761 ggatgcctcg gccaggcggg tggccagctg cgccatctcc gcgttgtagt acgcggggct
     5821 gcagttgatg agccgccgcg cctggaacgc gatcgtctcg gcgatgaccg cgggggagtg
     5881 gcccaggcag ttgaccgccc agccctggac gaagtcgaga taggtcttgc cctcctcgtc
     5941 ccggagccag gagccgtgcc cctcgaccat caccaccggc ggccggtcgg tgatcttcat
     6001 gagtgcggag acgtcggtgc tcatgccgtg tgctctcctt tggcgcggtt tgcgccgttg
     6061 cccgcgccca cgcggtaggg ctgcggctgc cgccgctcca tgaggcgatc ggggtagcgc
     6121 gcctcgcgga accccgactg gcggtagagc ccgtgggcgt cccgcgtgaa gagggtccag
     6181 aggcgcaggt tctgaagctc gggatggctc gtgaccgcgg ccatgatctg cttgccgatg
     6241 ccgcggccgc gatggctgtc gagcacgaag acgtcggcca cgtacgcgaa cgtcgcccgg
     6301 tcgctgatca cgcgggcgaa gcccacctga ccgccgcggt cgaaggcgcc gaagcagatc
     6361 gagtgacgga tggagcgctc caccacctcg cgcgggatgc cggcggccca gtaggagccg
     6421 gccagaaagc cgtgcacggt gtcgaggtcg agccgggtct catcggtgga gacgaggatg
     6481 tcgccgtcct ggcgctcgtg gatcgggggc cggatcatgg gctcgtcctt tcaggcgcgc
     6541 cggcccaccc gggccggggc caggggagcg cggaggcgcc gctcgaggcg cagggccgcc
     6601 gagggccccg gggtggccac gaagacggtg tcgtcccccg cgatggtgcc gatcacgtcg
     6661 gacagctccg tctcgtcgag ggcgcgggcc aggggatggg cgcccgcggg cggagtgcgg
     6721 atcaccacca gcgaggcggc ggcgcggacc gacagcacga actcgccgag cgcgcggtcg
     6781 agcgcgccct gccgggcctc ggtgggaacg aacgcgggcg cgggagtggt cacgtagccg
     6841 gtcggcgcct tggccaggcc cagggccttg agatcgcgcg agagcgtggg ctgcgcgacc
     6901 acgatgccct ggcgccgcag ccgccgctgc aggtcctcct gggagcgcac cccgccctcg
     6961 cccaccaccg cgaggatggc ccgccgccgc cggccgccct cgcctcctcc cgcgccgccc
     7021 ctcccgctcc cgtccgcata tatattcttc atggagaata taaatacatc aggacacccc
     7081 ggcggtcaag cggtagcgtg gctcgacgat gtcgacgcgc gcgtggtggg tgggagtgcc
     7141 ggcggccctg ctcgggctga tcgccctcgc ggcggtctat gccaccacca ccccgcgcta
     7201 cgacctctac cgcctgggcg ccgcggtgca gcggcacgac gtggacgacg ccctgcggta
     7261 cttcgacgtg gagcgcatcg ccgaccgcgc caccgacgtg ctggtggcgg actacctcgc
     7321 gcgccagcct gcgcccgcga cgccggccga ggccaatggg cgacagctgg tggcgagcct
     7381 cgccaagcgg cgcctccggc cgcaggtgat cgaccgggtc cgggtggagg tccggcggag
     7441 cgtggagcgg gccggtgccc cggcgggcgc ggtcgtcctg ccggtgggcg tggtggcggt
     7501 gctgaagggc ttcgacgtct cgcgggacgg tccggacgcc tgggtctcct accacgatcc
     7561 gaaggaggga cccgtccgct tccgcatggc gagacagccg ggcgagccgt ggaaaatcag
     7621 cgagttcgat cccgactggg tgcgccgtcg cgcgcgggag gatcccgtcc gccttcgctg
     7681 aggccccgaa tgccttgact cgccgcgaga gcctgtgata ttccactgtc cgggccgctg
     7741 tcgctcctcc catccggtcc ccacgaggtc accatggcgc aagtcgttgg attcattggg
     7801 ctcggaatca tgggccgtcc catggcgaag aacttgctca aggccggcta tccactgatt
     7861 gtccacagcc gaagccaggg gccggtccag gagctggtcg gggcgggggc caaggcggcc
     7921 gccacgccca gggatgtggc cgcgcagtgc gacgtggtga tcgcgatgtt gcccaactcg
     7981 ccggacgtgg agctggtcgc cctcggcaag gacggcctca tcgagggggc caagcgcggg
     8041 cagctcctgg ccgacatgtc cacgatctcg ccgctggtct cccagaaggt cggcaaggcc
     8101 ctcgcggcca agggcgtgcg catgctcgac gcgccggtgt cgggcggcga gaagggcgcc
     8161 atcgacgccg cgctctcgat catggtgggg ggcgagaagg ccgacttcga cgcggccctg
     8221 ccgatcttcc aggctctcgg caagaccatc acgcacctgg gtcccctcgg ggcgggcggc
     8281 ttcaccaagc tcgccaacca gatcatcgtg gcggtgaacc tgaccgcgct cggcgaggcg
     8341 ctcaccctgg cccgcaaggc ggggctcgac cgcgcgctca ccctgaaggc gctgggcggc
     8401 gggctcgcgg gctcgaagtg cctggagcag aagaccccga actacgtggg cgggacctac
     8461 aaccccggct tcaagatcga cctccacttc aaggacctcg gcctgatcat ggagtcctcc
     8521 cgcgcgctcg gggtcccgct gccgtgcacc gcggtggtgc aggagctgtt caacgccatg
     8581 cgggtgaagg gcaagggggg gctggatcac tccggcgtga tcacgctgct cgaggagctg
     8641 gcgggcgtgc cggagaaatc atgagggcga ccgtcgatcg tcccggtctc gactcgcagg
     8701 gccgctcgcc gctcatcaag atccgccgcg tccgcaaggg cgacctctcc aaggtgcggg
     8761 acgtcatcga gcaggccttc ggcgatttct tcgagcggca ggtcggcacc cgcccgcgcc
     8821 aggtcttcgg cggcgcccag tacgtgcacc accgctggct catggagccg tggggctgct
     8881 tcgtggccga ggagggcgac ggcaagatcg tgggggccgc catcgcggcc ctgtggggca
     8941 cggtcggggt catcggcccg gtcgcggtgc tgaccaacta ccagaaccag gacatcggcc
     9001 agcagctgct caccgcgtgc caggagttct tcgacgagaa caaggcgacg ctgcagggcc
     9061 tcgcgaccta cccgtacagc cccaagcacc tggtcctcta ccagaagttc ggctacaagc
     9121 cgaaggggct ggtggtcgtc accagcaagg cgctcgaccg gcccgactcg cccgcggcgg
     9181 cggcccggcc cgcgctcaag ccgggcctgg cggtgcgccg ctactcgacg ctcgaggagg
     9241 cgcgcaagaa gcaggtgatg cagaaggtcc ggcgcatcac caacggcttc tggcgcgggc
     9301 tcgacctcgc caaggagatc gagatcgtcg acgggctcac cctcggcgac acgctcctgc
     9361 tcgagaaggg gcgcgaggtg atcggcttcg cggtctgcca cctgccgccc aacagcgagg
     9421 cgccccacgg cagcgcctac gtgaagctcc tggccatcga cagccgccac cgcaagcccg
     9481 agcacctgca ggccctgctc cagggagtgg aggagatggc ctccgccgga cagctgcagc
     9541 gcgtggtggt gcccgcctac acgtactact ggaccgccta ccagacgctg ctggagcgcg
     9601 gctaccacgt ggacttcacc atggtgcgga tgaagcgggg taagcaggag gactacgaag
     9661 acgccagcga cctggtgctg gacgactggc gctgaccggc ccgtgacccg ctcgtcccgg
     9721 ccggcctccc tggcggcctt gctgctgtgg gtcccgctcg tcctgctgat ggtgtcgtcg
     9781 gcgtcgcctc acgcgatcgt gctcgagtcc tcgccggtcc acgacgcggt gctcgagcgc
     9841 gcgcccgggc aggtgaccct ccgcttcaac agcaagctcg agaagcgctt cacccggatc
     9901 accctcgcca ccggcgaccg gccacccgcg ccggttgcgg tgccgggcga cgacgacgcg
     9961 tccccgcccg accgcatcgt catccccctc tcgccgctcg cgccgggggt ctacgtgctg
    10021 cggtaccgcg tgctcgcggt ggacggccac atcaccgagg gcgccctgcg cttcaccgtc
    10081 ggcccgccgc ggtagcatgg cgcagctggc ggggtttctc gacgtcctcc tgcgcggctt
    10141 cgggacggtg gggctggccg cagcagtcgg cgggctcgcg tacatcctgc tggtgctgcg
    10201 gccgggcccg ggcgcaggcg gtctcggggg ggcggcgcgc gaccgggccg tcggcctgat
    10261 cgaggccggc gcgctgctgg tcgccgcgac caccgcactc ctgctgctgg tcgtccaccc
    10321 ctgggcgctg gccgatgaga ccggccggtg gccgctccgc gacttcctca ccacccactt
    10381 cggcctcgcc gagctcgggc gcgtgacgct ggccttgctg ctggtggccg cggtccgcgg
    10441 ggtgcggggc ggcgcggcga cccggggccg ctgggcgctc gcgctgggcc tcgggctcct
    10501 cctgatggcg aacgcggcct ggctcgcgca cgcggcgagc cgtctccacg agcgcgagcc
    10561 gctcatggtg gtgaccgtgt tgcatcagct cggcgcggtc gtgtgggtgg gcggcctcac
    10621 ccacctggtg ggcttcgcgc tgctctggcg ccgggcaggg gggcccgacg cggagccact
    10681 gggcgtgcgc gtgctcgcgc gcttctcggc ggtggccatc gccgcgctcg tgctggtcct
    10741 gggccccggc atcgtgctca cgtggagcta cgtcggcgcg tggggcggcc tcgtcggaac
    10801 cggctacggg gtgatggtgc tcaccaaggt ggcgctgctg gcctgcgcga tggtactggg
    10861 cggcctcaac tttctgctcc tccgcggggg cgggctcctc ctccgcgggg gcgggggcca
    10921 tcgcggcgcg gccgcggtgg ccgcgcgggt gcccgcgttc atcgaggcgg aggtgggttt
    10981 cggcatcacc ctcctgctgg ccgccgcctc gctgacctcg ctgccgccct cggtggacgt
    11041 ggtggccgat cgagccaccc cggccgaggt ggcggcgcgc ttccggcccg cgatgccgcg
    11101 gctgacctcg ccgccggtgg cgcagctgct ggccgcggcc gcgcccatgg aggacacgct
    11161 ggcccagcgg cagcccgagg agtacgcgtg gtccgaatac aaccaccacg cggccggctt
    11221 cttcgtgttc ctgatgggcg tgctcgcgct cgcggaccgc gcgggcttcg ttcgctgggc
    11281 gcgccactgg ccgctgctct tcctggggct ggccggcttc ctcttcgtcc gcaacgaccc
    11341 gcgggcctgg ccactgggcc ccgccggctt ctgggagagc atggtcctgc ccgacgtgct
    11401 ccagcatcgt ctggtcgtgc tgctcgtggt gtccctcggg ctcttcgagt ggatggtccg
    11461 ctcgggccgg ctcacccggc cgggcgggcg cctg
//
LOCUS       DATMCV010000210         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_877403, whole genome shotgun sequence.
ACCESSION   DATMCV010000210 DATMCV010000000
VERSION     DATMCV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877403"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VNU03_04910"
     CDS             <1..373
                     /locus_tag="VNU03_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73827.1"
                     /translation="LVTLLLGSAFGIVDGTVEAQVSGRPTDQDLRLEWTAAEDRRGRP
                     IVSGYVYNQRAGTYATSMRLQVEALGASGQAVGSTTGFVFGDVPPSGRSYFEIKAPAR
                     AASYRVTIQTFAWRAYGAGGG"
     gene            377..1492
                     /locus_tag="VNU03_04915"
     CDS             377..1492
                     /locus_tag="VNU03_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HWP73828.1"
                     /translation="MRLRSMFRLIVLFLVAPSLVTVGWPGAATAAAPAPDDPYVAGYA
                     GAVLEREFKVSRRAVTVQNGVLTLDAAQLAGADRARILTVLAALPGVSRVEVREPATP
                     VPPGRDPAAPADLSALPTGLLPVGHIFQPLLADPRWPHFSAAYRYYFGDRDLKHVGAV
                     SFGETIPIFRGGGLGTSQWEAGIQAGVFAVFQMDQPSKDLFNADYFAALYGAWRSGPF
                     STMGRLFHQSSHLGDEFLLRSRIERVNLTYESVDLKLSYDLPWGFRAYGGGGYLFDQE
                     PDLLPWSVQGGVEFRSPWTVASGHIRPVAALDLQSREQNGWNVDVSLRGGIQFENVRV
                     LDRNLQLLVEYFHGNSPDGQFFKRRVEYLGLGAHFHF"
     gene            complement(1489..2007)
                     /locus_tag="VNU03_04920"
     CDS             complement(1489..2007)
                     /locus_tag="VNU03_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP73829.1"
                     /translation="MPTVEALCRMLGLVPHPIEGGFFAETYRSAERALAAGLPPRYRG
                     PRALGTAIYYLLTPETFSAMHRLASDEVFHFYLGDPVEMLQLRPDRSHLVVVLGTDLP
                     AGERPQVVVPQGVWQGARLRHGGRLALLGTTVAPGFDYADYEIGDRAALLASHPGARD
                     LVTALTRPAATG"
     gene            2045..2488
                     /locus_tag="VNU03_04925"
     CDS             2045..2488
                     /locus_tag="VNU03_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWP73830.1"
                     /translation="MRRIRRILHATDFSRASTPALRWAIAMAKANRARLTVVHVMAPP
                     ALALPGEGYVSPTLYESLESSARAAAQKRLKVVVGRAKTAGVRASVLLLEGVPHELIT
                     RAARKADLLVIGTHGRSGLAKLFLGSVASRLVAVAPCPVLTVRGR"
BASE COUNT          331 a          904 c          917 g          358 t
ORIGIN      
        1 gctggtcacg ctgctgctcg gctcggcttt cggaatcgtg gacggaaccg tcgaggccca
       61 ggtgtcgggc cgtccgacgg accaggacct gcggctcgaa tggaccgccg cggaagaccg
      121 ccgcgggcgg cccatcgtga gcggctacgt ctacaaccag cgcgcgggga cctacgcgac
      181 ctcgatgcgg ctccaggtgg aggccctcgg cgcctccggc caggcggtgg gctcgacgac
      241 cggcttcgtg ttcggcgacg tgccgccatc cggccgctca tacttcgaga tcaaggcgcc
      301 cgccagggcg gcgagctacc gggtcacgat ccagaccttc gcgtggcgtg cctacggggc
      361 gggaggaggc tagcgcatgc ggctccgatc gatgttccgg ctcatcgtgc tgttcctcgt
      421 cgcgccatcg ctcgtcaccg tcggatggcc cggcgccgcg accgccgccg cgccggcgcc
      481 ggacgacccc tacgtggccg gctacgcggg ggccgtgctc gagcgcgagt tcaaggtgag
      541 ccgccgcgcg gtgaccgtgc agaacggggt gctgacgctc gacgccgcgc agctcgccgg
      601 cgcggaccgc gcccgcatcc tgaccgtgct cgccgcgctg cccggggtga gccgggtgga
      661 ggtgcgcgag ccggccacgc cggtcccgcc gggccgcgac ccggccgcgc ccgccgacct
      721 ctccgcgctg cccaccgggc tgctcccggt cggccacatc ttccagccgc tgctcgccga
      781 cccccgctgg ccgcacttct ccgccgccta ccgctactac ttcggcgatc gagatctcaa
      841 gcacgtgggc gcggtcagct tcggcgagac catccccatc ttccgcggcg gcggcctcgg
      901 gacgagccag tgggaggcgg gcatccaggc cggcgtgttc gcggtgttcc agatggatca
      961 gccgtctaag gacctgttca acgccgacta cttcgccgcg ctctacggcg cctggcgcag
     1021 cggcccgttc tcgacgatgg gccggctgtt ccaccagagc tcgcacctgg gcgacgagtt
     1081 cttgctgcgc tcgcggatcg agcgcgtcaa cctgacctac gagagcgtgg acctcaagct
     1141 ctcgtacgac ctgccgtggg ggttccgcgc ctacggcggc ggcggctacc tcttcgacca
     1201 ggagccggac ctcctgccgt ggtcggtgca gggtggcgtc gagttccgga gcccgtggac
     1261 ggtggcctcc ggccacatcc gcccggtggc cgcgctcgat ctacagagcc gcgagcagaa
     1321 cggctggaac gtggacgtct cgctgcgcgg cggcatccag ttcgagaacg tccgcgtgct
     1381 cgatcgcaac ctgcagctcc tggtcgagta cttccacggc aactccccgg acgggcagtt
     1441 cttcaagcgg cgcgtcgagt acctggggct cggggcgcac ttccacttct agccggtcgc
     1501 ggccgggcgg gtcagagcgg tcacgaggtc gcgggcgccc gggtggctcg ccaggagggc
     1561 ggcgcggtcg ccgatctcgt agtcggcgta gtcgaagccg ggcgcgacgg tggtgccgag
     1621 gagggcgagc cggcccccgt ggcggagccg cgccccctgc cacacgccct gcggcaccac
     1681 gacctgcggc cgctcgccgg ccggcaggtc ggtcccgagc accaccacga ggtgggagcg
     1741 gtcgggccgg agctgcagca tctccaccgg atcgcccagg tagaagtgga acacctcgtc
     1801 cgaggccagg cggtgcatgg cggagaaggt ctccggggtg agcagatagt agatcgcggt
     1861 tccgagcgcg cgcggtccac ggtagcgcgg cggcaggccg gcggcgagcg ctcgctcggc
     1921 gctccggtag gtctccgcga agaacccgcc ctcgatcgga tgggggacga ggccgagcat
     1981 ccggcagagc gcctcgacgg tcggcatgcg gcattgtagc aaggaggtgt agtaagctac
     2041 cgccatgcgc cgcatccgtc ggatcctgca cgcgaccgat ttctcccggg cttccacccc
     2101 cgcgctccga tgggcgatcg cgatggccaa ggccaatcgc gcccgcctca cggtggtgca
     2161 cgtgatggcg ccgccggcgc tggccctgcc cggcgaaggc tacgtgtccc ccacgctcta
     2221 cgagagcctc gaaagctcgg cccgcgccgc cgcccagaag cggctcaagg tggtggtggg
     2281 ccgggccaag accgcgggcg tgcgcgcgag cgtgctcctg ctcgaaggcg tgccccacga
     2341 gctgatcacg cgggccgcgc ggaaggcgga cctgctcgtg atcggtaccc acggccgcag
     2401 cggcctcgcg aagctgttcc tcggcagcgt ggcgagccgc ctggtggcgg tggcgccctg
     2461 cccggtgctg acggtgcggg gccggtaggc gatggacgtc gcgacgccgg
//
LOCUS       DATMCV010000211         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_745915, whole genome shotgun sequence.
ACCESSION   DATMCV010000211 DATMCV010000000
VERSION     DATMCV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745915"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VNU03_04930"
     CDS             <1..372
                     /locus_tag="VNU03_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73831.1"
                     /translation="AEGSYHILFFLPWFPEAMPDGKLAKAFVDEWAKRGHPFEGLTEG
                     FRGHDGVLTAVEAIRIAGKAEPKAVQAALWQVSVMGLNGPIKFEKDGPAGKESGQSKP
                     SIFIVQVKDGKIALPAFAAKK"
     gene            395..1252
                     /locus_tag="VNU03_04935"
     CDS             395..1252
                     /locus_tag="VNU03_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73832.1"
                     /translation="MDQLLQHLLNGLLLGATYSLLGIGLTLVFGLMSVVNFAHGEFYT
                     LGAYAAFAALTLASVHFFLAIPLAIAAGAVAGAICERVLLRPLRGQSIDTVMLVMIGV
                     WIAMQNAELLGWGGVAKSVPTPFPTQPLVLGPVSVAPLRVFVFVVSGGLILGAHLLLS
                     RTKLGRAMRATFQDRETAALMGVSIERIHTITFAFGAGLAAAAGALLGPVFLLYPSMG
                     DLASLKAFSVVILGGLGNMLGCLAAGLLLGLVETAGVYLASSDIRLISSYAVLSLILI
                     LRPSGLFGR"
     gene            1242..2159
                     /locus_tag="VNU03_04940"
     CDS             1242..2159
                     /locus_tag="VNU03_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73833.1"
                     /translation="MAARLGWAALGAALVVAPQVLGPYYRSLALAILADVALAVAWAF
                     FSGPTRYLSLATGAFFGAGAYTTAVVLGRLPWPLPVLAAAAVAAAMALLVGTLALRLR
                     GPYFAVFTFGLAELAKHTLIWFETSITGTVGRLLLSPPGPIALYYTVLAVAALAVTTA
                     VALPRSRWGVALIGIGADEERAETLGIDTTRVKIGVFALSAAFMGAIGGAMAPRWTYL
                     DPQVFNPLVSFQTVIMALLGGPATVAGPILGAVFLGLLSEILLVKFRYLYMLTLGLVL
                     IVVVLVLPGGLAGLVRRRRPACGAARATL"
     gene            2179..>2523
                     /locus_tag="VNU03_04945"
     CDS             2179..>2523
                     /locus_tag="VNU03_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWP73834.1"
                     /translation="MPRRPVIDNTKVAAVFRSYPRGVAAKLLALRRLILDVAASTEGV
                     GEVYEALRWGQPSYLTVRPRSGSTIRIDRIKSRPGRYAMYFHCQTTLVETFRGQYPST
                     FRYEGNRAIVFDE"
BASE COUNT          296 a          912 c          871 g          444 t
ORIGIN      
        1 gccgagggct cgtaccacat cctgttcttc ctgccctggt tcccggaggc gatgccggac
       61 ggcaagctcg cgaaggcctt cgtcgacgag tgggccaagc gcggccaccc cttcgagggg
      121 ctgaccgagg gcttccgcgg ccacgacggc gtgctcaccg cggtggaggc catccggatc
      181 gcgggcaagg cggagccgaa ggcggtacag gccgcgctct ggcaggtgag cgtgatgggg
      241 ctcaacgggc cgatcaagtt cgagaaggac ggtccggccg gcaaggagag cgggcagtcc
      301 aagcccagca tcttcatcgt ccaggtgaag gacggcaaga tcgcgctgcc cgcgttcgcg
      361 gccaagaagt agcgcgggag gcgaccgcga gcgcttggac cagcttctcc agcacctcct
      421 gaacggattg ctgctggggg ccacctacag cctgctcggg atcggcctca ccctggtctt
      481 cgggctgatg agcgtggtga acttcgccca cggcgagttc tacacgctgg gagcctacgc
      541 cgccttcgcg gcgctgacgc tggcctcggt gcacttcttc ctcgcgatcc ccctcgcgat
      601 cgccgccggc gcggtggcgg gcgcgatctg cgagcgggtt ctcctgcgcc ccctgcgcgg
      661 ccagtcgatc gacaccgtga tgctggtgat gatcggggtc tggatcgcca tgcagaacgc
      721 ggagctgctc ggctggggcg gggtcgccaa gagcgtgccg acgccgtttc cgacccagcc
      781 gctggtgctg ggcccggtct cggtcgcccc gctgcgggtg ttcgtcttcg tggtctcggg
      841 gggtctgatc ctcggcgcgc acctgctcct ctcacggacc aagctcggtc gggccatgcg
      901 ggcgaccttc caggatcgcg agaccgccgc cctcatgggg gtgagcatcg agcgcatcca
      961 caccatcacc ttcgcgttcg gggccggcct ggccgccgcg gcgggggccc tgctgggtcc
     1021 cgtgttcctg ctctatccct ccatgggcga cctggcctcg ctcaaggcct tctcggtggt
     1081 catcctgggc gggctcggca acatgctcgg ctgcctggcc gccggcctgc tcctcgggct
     1141 ggtcgagacc gccggggtct acctcgcgtc gtcggacata cggctcatct cgtcctacgc
     1201 ggtgctgtcg ctcatcctga tcctccggcc gtcgggcctc tttggccgct aggctcggct
     1261 gggccgcgct cggcgccgcc ctggtggtcg cgccccaggt gctgggcccg tactaccgct
     1321 cgctcgcgct cgccatcctg gccgacgtcg cgcttgcggt cgcctgggcc ttcttctccg
     1381 gcccgacgcg ctatctctcg ctcgccaccg gcgccttctt cggcgccggg gcctacacca
     1441 ccgcggtggt gctggggcgg ctgccgtggc cgctcccggt gctggccgcc gcggcggtgg
     1501 cggcggcgat ggcgctgctg gtgggcacgc tcgccctgcg gctgcgcggg ccctacttcg
     1561 cggtgttcac cttcgggctg gccgagctgg ccaagcacac cctcatctgg ttcgagacgt
     1621 ccatcaccgg cacggtcgga cggctcctgc tgagcccgcc cggcccgatc gccctctact
     1681 acacggtgct cgcggtcgcc gccctcgcgg tgaccaccgc ggtggcgctg ccccgcagcc
     1741 gctggggcgt cgccctgatc ggcatcgggg ccgacgagga gcgcgcggag accctcggca
     1801 tcgacaccac ccgcgtcaag atcggggtct tcgcgctgag cgccgcgttc atgggcgcga
     1861 tcggcggggc gatggccccg cgctggacct acctcgatcc ccaggtgttc aatcccctgg
     1921 tgtccttcca gaccgtgatc atggccctcc tgggcgggcc ggccaccgtc gcgggcccga
     1981 tcctgggcgc ggtcttcctc ggcctcctgt ccgagatcct cctcgtgaag tttcgctacc
     2041 tctacatgct gaccctgggg ctggtgctga tcgtggtggt gctggtgctg ccgggaggcc
     2101 tcgccggcct cgtgcggaga cggcgcccgg cttgcggcgc cgcgcgcgcg acgctatagt
     2161 cgcgctcgga gtcgagtcat gccacgtcga cccgtcatcg acaacacgaa ggtcgccgcc
     2221 gtcttccgct cgtatccgag gggcgtcgcg gccaagctcc tggctctccg tcgattgatc
     2281 ctcgacgtgg cggcctcgac cgagggagtg ggcgaggtgt acgaggccct ccggtggggc
     2341 cagccgagct atctcaccgt gcggccccgg agcggcagca cgatccgcat cgaccggatc
     2401 aagtcccggc ccgggcgcta cgcgatgtac ttccactgcc agaccacgct ggtcgagact
     2461 ttccgcggtc agtatccgag caccttccgt tacgaaggca accgtgccat cgtgttcgac
     2521 gag
//
LOCUS       DATMCV010000212         2314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_146422, whole genome shotgun sequence.
ACCESSION   DATMCV010000212 DATMCV010000000
VERSION     DATMCV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146422"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VNU03_04950"
     CDS             <1..1294
                     /locus_tag="VNU03_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWP73835.1"
                     /translation="RSASSSSRLDARGAGRVLSCPAMEAPPPRQAASHALWERARRVM
                     PGGVYGHQNGAALGEGHPRFLARAERARVWDVDGNEYVDWMCAYGPMILGYGHPAVEA
                     AVAEQVAAGDCLPLPGPRMVELAERLVALTPRADLVVFGKNGADATSWAVDVARAHTG
                     RPRIVRVRGAYHAARPWALPHLPGVPAHYRDDLLEFAWNDPDELRARFTAHPGEIAVV
                     MTTPFDHQTGAAPAPGFFAGLRALCDEHGALFAMDDVRAGFRFHLGGSCEHWGAAPDL
                     ICYSKAIANGHPLSAALAREALRGAAERIYFTGSFFFASGAFAAALATLAVLERTDAI
                     AQIQRIGGLLVDGLAEQARRHGVPMLPPGQPAMPVIRFPEDSERRRIRRWCSLVTQGG
                     AYAHPGHNWFVSAAHTEEDVARTLEATEGAFRTIAAEA"
     gene            complement(1365..>2314)
                     /locus_tag="VNU03_04955"
     CDS             complement(1365..>2314)
                     /locus_tag="VNU03_04955"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HWP73836.1"
                     /translation="DYVKKARAFGMVMKRTDPSIQLVGCGLNGWSRWDEVVLDGLAEM
                     VDFHSIHLYTGGVDHYTTVMQSHQAERAMRICAALIERVRHMRQIAHPIHIAFDEWNV
                     WWRTRSHEDRVGGVEERYTLTDALAVATYLHGFIRQCRVVRMANFAQLVNAIAPIFTR
                     RQGLFLQTIYHPLRLYAEHTRPVALDVHVDGPQYALPPERETDEAGGRVHHVSDLGPF
                     SLIDAVASCDEAGREITVALVNRDRDRDQPATIEVAGADVAGGVDGWEVNGPEVGATN
                     SFEHPTRVDVQERRATARSGGLDYVCPAHSITLLRLRAR"
BASE COUNT          287 a          885 c          817 g          325 t
ORIGIN      
        1 gcgctcggcc tcgagcagta gtcggctcga cgcccgcggc gcgggccggg tgctatcatg
       61 cccggccatg gaagccccgc ctccccgcca ggccgcgagc catgccctct gggagcgcgc
      121 tcgccgcgtg atgccgggcg gcgtctacgg ccaccagaac ggcgcggccc tgggcgaggg
      181 ccacccgcgc ttccttgccc gggccgagcg ggcgcgggtg tgggacgtcg acggcaacga
      241 atacgtcgac tggatgtgcg cctacggccc gatgatcctg ggctacggcc atcccgcggt
      301 ggaggcggcg gtcgccgagc aggtggccgc gggcgactgc ctgccgctgc ccgggccgcg
      361 catggtcgag ctggccgagc ggctcgtcgc gctcaccccg cgcgccgacc tggtcgtgtt
      421 cggcaagaac ggcgccgacg cgacgagctg ggccgtcgac gtggcgcgcg cccacaccgg
      481 ccggccgcgc atcgtccggg tgcgtggcgc ctatcacgcg gcgcgcccct gggccctccc
      541 ccacctgccc ggggtgcccg cccactaccg cgacgacctg ctcgagttcg cgtggaacga
      601 tccggacgag ctgcgggccc gcttcaccgc gcatccgggc gagatcgcgg tggtgatgac
      661 gacgccgttc gaccaccaga ccggcgccgc gcccgcgccg ggcttcttcg ccggcctgcg
      721 cgccctctgc gacgagcacg gcgcgctctt cgcgatggac gacgtgcgcg ccggctttcg
      781 cttccacctg ggcggctcgt gcgagcactg gggcgcggcc ccggatctca tctgctactc
      841 caaggccatc gccaacggcc atccgctgtc cgccgcgctc gcccgcgagg cgctccgggg
      901 cgccgccgag cgcatctact tcaccggctc cttcttcttc gcgagcggcg ccttcgcggc
      961 cgcgctggcc accctcgcgg tgctcgagcg caccgacgcc atcgcgcaga tccagcggat
     1021 cggcggcctg ctcgtggacg gcctcgccga gcaggcccgg cgtcacgggg tcccgatgct
     1081 cccgccgggt cagcccgcga tgccggtgat ccgcttcccg gaggactccg agcgccggcg
     1141 catccgccgc tggtgctcgc tcgtcaccca gggcggggcc tacgcgcatc cgggccacaa
     1201 ctggttcgtc tcggccgccc acaccgagga ggacgtggcg cgcaccctgg aggcgaccga
     1261 gggcgcgttc cggacgatcg cggccgaggc ctagcccctc accctctagc ccctcaccct
     1321 aaccctctcc cctcagggga gagggcaggg tgaggggccg aagatcagcg cgcgcggagc
     1381 cggagcaagg tgatggagtg ggccgggcac acgtagtcga gcccgccgct ccgcgcggtg
     1441 gcccggcgct cctgcacgtc cacccgggtg ggatgctcga acgagttggt ggcccccacc
     1501 tccggcccat tcacctccca gccgtcgacc ccgcccgcca cgtccgcccc cgccacctcg
     1561 atggtcgccg gctggtcccg gtcccggtcg cggttgacca gggccaccgt gatctcgcgc
     1621 ccggcctcgt cgcagctcgc gaccgcgtcg atcagcgaga acggcccgag atccgagacg
     1681 tggtgcactc gccccccggc ctcgtcggtc tcgcgctcgg gcggcagcgc gtactgcggc
     1741 ccgtccacgt gcacgtcgag ggcgaccggc cgcgtgtgct ccgcgtagag ccggaggggg
     1801 tggtagatgg tctggaggaa caggccctgg cggcgggtga agatcggggc gatggcgttg
     1861 acgagctggg cgaagttggc catgcgcacc acccggcact gacggatgaa gccgtgcaga
     1921 taggtcgcca ccgccagggc gtcggtgagg gtgtagcgct cctccacccc gcccacccgg
     1981 tcctcgtggc tgcgggtccg ccaccacacg ttccactcgt cgaaggcgat gtggatcggg
     2041 tgcgcgatct gccgcatgtg ccgcacccgc tcgatcagcg cggcgcagat gcgcatcgcg
     2101 cgctccgcct ggtgcgactg catgacggtg gtgtagtggt cgacgccccc ggtgtagagg
     2161 tggatcgagt ggaagtcgac catctccgcg aggccgtcga gcaccacctc gtcccaccgg
     2221 ctccagccgt tcaggccgca gcccaccagc tggatggacg ggtcggtgcg cttcatcacc
     2281 atgccgaacg cgcgcgcctt cttcacgtag tcgt
//
LOCUS       DATMCV010000213         8498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_674904, whole genome shotgun sequence.
ACCESSION   DATMCV010000213 DATMCV010000000
VERSION     DATMCV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674904"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VNU03_04960"
     CDS             <1..255
                     /locus_tag="VNU03_04960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939308.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWP73837.1"
                     /translation="FMLVMDGYGIRPPAGEAYFAAEGANGELGFYVVSDGSDRPYRVH
                     CRPPCLPPVAALPKMIEGEMVADIIPTFGSVNMIGGELDR"
     gene            259..744
                     /locus_tag="VNU03_04965"
     CDS             259..744
                     /locus_tag="VNU03_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWP73838.1"
                     /translation="MPDARRPEFTPEQLGEVRRLQGLYPDKKGALLPVLRLAQDVFGY
                     VSLEVEEYVAGLFEMSPAHVHEVVTFYTLFFQQPPGRHVVSVCHNLTCHLLGSKSIVE
                     HLTRRLGIEVGETTADGRVTLLTVECLCACEQAPMMQVDERYEGLLTPEKVDRIIEGL
                     A"
     gene            744..1994
                     /gene="nuoF"
                     /locus_tag="VNU03_04970"
     CDS             744..1994
                     /gene="nuoF"
                     /locus_tag="VNU03_04970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWP73839.1"
                     /translation="MAELIFTRNFDRPGSHTLAIYRETGGYTALPKALQMEPTAITEE
                     VKKSNLRGLGGAAFPTGVKWSFIPKGSTAPKYLVINADEGEPGTFKDRYLMERDPHAM
                     IEGMIIAGRAIDSHLAFVYIRGEYVKPWQIVSGAVKEAYETGLLGKNIQGSGFDFDIV
                     VHRGAGAYICGEETGLLSSLEGKKGWPKIKPPFPAIKGAFGQPTIVNNVETIAAVPHI
                     INRGGEWFAGLGTKTQGGTRLYSVSGHVVKPGVVEVPVSVTLRSLIYDHCGGMRDGRR
                     LKAVVPGGSSAPILTADEIDVTMDADGLKAAGTMIGSAGVIVMDETVSIPEALMVVAR
                     FYAHESCGQCTPCRESTGWIYKIAHRIVEGKGRKEDLDTILDVSKRGAGTTICAFYDG
                     AVGPYVSYIEKFRGEFEALIPHDA"
     gene            2003..3601
                     /locus_tag="VNU03_04975"
     CDS             2003..3601
                     /locus_tag="VNU03_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP73840.1"
                     /translation="MPKLTIDGKEVEVAPGTNLIEAARQAGSEVPHYCYHPGLSIAGQ
                     CRLCMVDIDKNPRPQIGCNTQAADGMVVHTQTERVLETRKSIMEFHLVNHPLDCPVCD
                     QAGECWLQIYYMRHGLYDPRMTDEKVHKPKAVPLGPHVMLDAERCILCSRCVRFCDEV
                     TGGGELGIFNRGDHSEIGLFPGKTLENKYSGNVIDICPVGALTDRDFRFQVRVWYLDT
                     ARSVCNGCARGCNVEVHTNRRRPHHNQGRRIARIKPRHNPDVNQWWMCDDGRYGFRWI
                     DDESRLLVPTRRAAGKDVALGWDEAVTTLVEALRRYRPEEIGILASPQMSNEDLFALQ
                     RVAGQLGIGGIDHQVPPRVAGDEDNLLIRADKNPNRRGAELIGLAQADGHAILDGARQ
                     RRVKLLWVFQHDLLDSGWPQADVTAALAAAEMVVFQGPSANATSAGAHLVLPSAAYVE
                     REGTFTNFQGRVQRFRTVVEPLGEAMADWSILGRVGRALGGTDPVFAATRAEQVFAGL
                     AGQVPALSGMSYRGLRDAGQVVKA"
     gene            3598..4722
                     /locus_tag="VNU03_04980"
     CDS             3598..4722
                     /locus_tag="VNU03_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HWP73841.1"
                     /translation="MSGLALDLLLQAIPVGFVMFLVLNFGGLLTWVERKQSAIMQDRI
                     GANRASISVLGFRIRILGLLHPLADAVKMLTKEDFTPARADVLLFKLAPFVSVFFGLA
                     AFASIPFGDTLQIMGREIQLQAVTLNVGILYVLAMISLGVYGLMMAGWASANNYALLG
                     GQRAAALMISAEIAIGASIMGVVMVYGSLNMQDIARGQGQPLLPQFLGNWIPAWGVLT
                     QPAAFILFLTAGIASTKRIPFDTPEGESEIIGYFVEYSGMKFGMFAMADFLETVVIAG
                     MTTALFLGGWQVPYLQASGFVFPWGASVALPHLAVVGLQVVSFLTKVVVMLWFLMLIR
                     WTLPRFRYDQAMRLGWLALFPLSVLNILVTALVLLAVGKG"
     gene            4726..5322
                     /locus_tag="VNU03_04985"
     CDS             4726..5322
                     /locus_tag="VNU03_04985"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWP73842.1"
                     /translation="MAYQTENTPADPRHSSRLGLRQRFYVVEVLVGLALTSQHFFANM
                     WRHTLKVVFRSKTARGAVTIQYPDERRPYSPRLRSLHRLVRREDGSPRCVACMMCETV
                     CPAHCIYIVATEHPNPEIEKVPERFDIDLGKCVFCGFCVEACPEDAIRMDTGILEFAA
                     YDRASMVYTKDTLLALEAAGADGVPTSPVPIPPSRRPA"
     gene            5319..5810
                     /locus_tag="VNU03_04990"
     CDS             5319..5810
                     /locus_tag="VNU03_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWP73843.1"
                     /translation="MSFVTFFVLAALAVGSAAGLLLRRNPIHGALFLVVNLGTIAALF
                     LTLRAEFLAAVQVIVYAGAIAVLFVFAIMVLIPGKEETGPDPLRGQRLLSVPVVGVFL
                     ALVAVVVGSATLAGSPKPPVAGGADAVGRVLFTDYLFPFEVTSVLLLAAIVGVMALTK
                     RKA"
     gene            5819..6121
                     /gene="nuoK"
                     /locus_tag="VNU03_04995"
     CDS             5819..6121
                     /gene="nuoK"
                     /locus_tag="VNU03_04995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWP73844.1"
                     /translation="MVPESYYVALSAVLFSMGVLGVLIRRNPLVIFMSIELMLNAANL
                     ALVAFGQRHGTTEGQALVFFVMCVAAAEVAVGLAIIVAIFRQRRRLSVDELSTLRG"
     gene            6172..7170
                     /gene="pdhA"
                     /locus_tag="VNU03_05000"
     CDS             6172..7170
                     /gene="pdhA"
                     /locus_tag="VNU03_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HWP73845.1"
                     /translation="MAQTKTAPPPTRLDGELARRLLTQMALIRRFEEKAAEMYALGKV
                     GGFLHLYIGQEAVAVGATSICRPDDYAVSSYREHGHCLAKGSDPRRMMAELFGRMGGL
                     SHGKGGSMHLFDKSVNFLGGHGIVGAHLPLAAGAGFAIKYQGGDQVVLCYFGDGAVPE
                     GEFHESMNLAALWKLPVIFICENNRYAMGTAIHRALAQTEIWRFAETYGIPGEAVDGM
                     DVLAVRDTVGRAVERARRDKTPTLVEARTYRFRGHSMRDPAGSVYRTKEEVEREKLRD
                     PIALFTDRCLKEGALTEADLKNIEKAVNDLADEAVAFAEASPEPPPEELFTDVYKD"
     gene            7175..8155
                     /locus_tag="VNU03_05005"
     CDS             7175..8155
                     /locus_tag="VNU03_05005"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HWP73846.1"
                     /translation="MSVMTYREALNVALREEMRRDPRVFVMGEEVGLYDGAYKVTQGL
                     LKEFGDKRIIDTPICESGFTGVGVGAAMLGLRPVIEMMTFNFSILALDQIVNSAAKMC
                     YMSGGQYNIPLVVRGPGGPAAQLAAQHSQSMETYFYHVPGLKVVRPSTPADAKGLLKS
                     AIRDDNPVIFIEAETLYAVKGEVPDDPDFLIPLGQAVIRREGTDVTVVAYMGMLYRAL
                     EVAEELAKEGISVEIVDPRTLRPMDADTIIGSVRKTHRAVVLEAGAGFAGMGSEIAAQ
                     ITENAFDDLDAPVERVTGENAPMPYAKNLELLKTPSKAKIAAAIRRVCNR"
     gene            8152..>8498
                     /locus_tag="VNU03_05010"
     CDS             8152..>8498
                     /locus_tag="VNU03_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWP73847.1"
                     /translation="MITKVIMPKLSDAMETGKVIKWLKKEGEAVKGGDILVEVETDKA
                     NVEVEAFGSGVLRKIVIAEGDQVPVGDLIAVIADAAEDISSVAATPAKPAPPAPAAPA
                     AAAPAAPAPAPRPA"
BASE COUNT         1288 a         2949 c         2948 g         1313 t
ORIGIN      
        1 ttcatgctgg tgatggacgg ctacggcatc cgtccccccg cgggcgaggc ctacttcgcg
       61 gcggaggggg cgaacggaga gctgggcttc tacgtggtgt cggacggcag cgaccgcccc
      121 taccgcgtgc actgccggcc cccgtgcctg cccccagtgg ccgcgctgcc caagatgatc
      181 gagggcgaga tggtggccga catcatcccc accttcggct ccgtcaacat gatcggcggc
      241 gagctggacc gctagcccat gccagacgcg cggcggcctg agttcacgcc cgagcagctc
      301 ggcgaggtcc ggcggctgca ggggttgtat cccgacaaga agggcgcgct cctaccggtg
      361 ctgcggctgg cccaggacgt gttcggctac gtgtcgctcg aggtggagga gtacgtggcc
      421 gggctcttcg agatgtcgcc cgcgcacgtc cacgaggtcg tgaccttcta caccctcttc
      481 ttccagcagc cccccggccg ccacgtggtc tcggtgtgcc acaacctcac ctgccacctg
      541 ctggggtcga agtcgatcgt cgagcacctg acccggcgtc tgggcatcga ggtcggggag
      601 acgaccgcgg atgggcgggt cacgctcctg accgtggagt gcctgtgcgc gtgcgagcag
      661 gcgccgatga tgcaggtgga cgagcgctac gagggactgc tcaccccgga gaaggtggac
      721 cggatcatcg aggggctcgc ctgatggcgg agctgatctt cacccggaac ttcgaccgcc
      781 cgggctccca caccctcgcg atctaccgcg agaccggcgg ctacaccgcg ctccccaagg
      841 cgctccagat ggagccgacc gcgatcaccg aggaggtgaa gaagtccaac ctccgtgggc
      901 tggggggcgc cgcgttcccc accggggtca agtggagctt catccccaag ggctcgaccg
      961 cccccaagta cctcgtgatc aacgcggacg agggcgagcc gggcaccttc aaggaccgct
     1021 acctgatgga gcgcgatccg cacgccatga tcgagggcat gatcatcgcg gggcgcgcca
     1081 tcgactccca cctggccttc gtttacatcc gcggcgagta cgtcaagccg tggcagatcg
     1141 tgagcggggc ggtgaaggag gcctacgaga cgggcctgct cggcaagaac atccagggct
     1201 cgggcttcga cttcgacatc gtggtccatc gcggggcggg agcctacatc tgcggcgagg
     1261 agaccgggct cctgtcctcg ctcgagggca agaagggctg gcccaagatc aagccgccgt
     1321 tcccggccat caagggcgcc ttcgggcagc ccaccatcgt gaacaacgtg gagaccatcg
     1381 ccgcggtgcc ccacatcatc aaccgcggcg gggagtggtt cgccgggctc ggcaccaaga
     1441 cccagggcgg gacgcggctc tactcggtgt ccggccacgt ggtgaagccg ggcgtggtgg
     1501 aagtgccggt gtcggtgacc ctccgctcgc tgatctacga tcactgcggc ggcatgcgcg
     1561 acgggcgccg gctcaaggcg gtggtccccg gcggctcctc ggcgccgatc ctcaccgcgg
     1621 acgagatcga cgtgaccatg gacgccgacg gcctcaaggc ggccggcacc atgatcggct
     1681 cggcgggggt catcgtgatg gacgagaccg tctcgatccc ggaggccctg atggtggtgg
     1741 cgcgcttcta cgcccacgag tcgtgcgggc agtgcacgcc gtgccgcgag tcgaccggct
     1801 ggatctacaa gatcgcccac cggatcgtgg aggggaaggg ccgcaaggaa gatctggaca
     1861 ccatcctgga cgtgtccaag cgcggggccg gcaccaccat ctgcgccttc tacgacggcg
     1921 cggtgggccc ctacgtgagc tacatcgaga agttccgcgg agagttcgag gccctgatcc
     1981 cccacgatgc ctagaccgac cgatgcctaa gctcacgatc gacggcaagg aagtcgaggt
     2041 cgccccgggc accaacctca tcgaggcggc ccggcaggcc ggctccgagg tgccccacta
     2101 ctgctatcac cccgggctca gcatcgcggg ccagtgccgg ctctgcatgg tggacatcga
     2161 caagaacccg cgcccgcaga tcggctgcaa cacccaggcc gccgacggga tggtcgtgca
     2221 cacgcagacc gagcgcgtgc tcgagacccg caagtcgatc atggagttcc acctcgtcaa
     2281 tcacccgctg gactgcccgg tgtgcgacca ggcgggcgag tgctggctgc agatctacta
     2341 catgcggcac ggcctctacg acccgcgcat gaccgacgag aaggtccaca agccgaaggc
     2401 ggtgccgctc gggccccacg tcatgctgga cgcggagcgc tgcatcctct gctcccgctg
     2461 cgtgcgcttc tgcgacgagg tgaccggcgg gggcgagctc ggcatcttca accgcggcga
     2521 ccactccgag atcggcctct tcccgggcaa gaccctcgag aacaagtact ccggcaacgt
     2581 gatcgacatc tgcccggtgg gcgccctcac cgaccgcgac ttccgcttcc aggtgcgcgt
     2641 gtggtacctc gacaccgcgc gctcggtctg caacgggtgc gcgcggggct gcaacgtcga
     2701 ggtgcacacc aaccgccgcc ggccgcacca caaccagggc cggcgcatcg cgcggatcaa
     2761 gccgcgccac aatcccgacg tcaaccagtg gtggatgtgc gacgacggcc gctacggctt
     2821 ccggtggatc gacgacgaga gccggttgct ggtccccacg aggcgtgcgg cgggcaagga
     2881 cgtcgccctc ggctgggacg aggcggtcac gacgctggtg gaggccctcc ggcgctaccg
     2941 cccggaggag atcggcatcc tcgcctcgcc ccagatgtcg aacgaggacc tcttcgccct
     3001 gcagcgggtc gcgggccagc tcgggatcgg cgggatcgac caccaggtcc cgccgcgggt
     3061 ggccggcgac gaggacaacc tcctcatccg cgcggacaag aaccccaacc ggcggggggc
     3121 cgagctgatc gggctggccc aggcggacgg ccacgccatc ctggacgggg cccggcaacg
     3181 gcgcgtcaag ctcctgtggg tcttccagca cgacctgctc gattcgggct ggccccaggc
     3241 cgacgtgacc gcggccctgg ccgcggccga gatggtggta ttccagggcc cgagcgcgaa
     3301 cgcgaccagc gccggggcgc acctggtgct cccgagcgcg gcctacgtcg agcgcgaggg
     3361 caccttcacc aacttccagg ggcgggtgca gcgcttccgc accgtggtgg agccgctcgg
     3421 cgaggcgatg gcggattggt ccatccttgg ccgggtcggg cgcgccctcg gaggcacgga
     3481 tccggtcttc gcggcgaccc gcgccgagca ggtcttcgcg ggcctggccg gccaggtgcc
     3541 cgccttgtcc ggcatgagct accgcgggct gcgcgacgcg ggccaggtgg tgaaggcgtg
     3601 agcggcctcg ccctggatct cctgctgcag gcgatcccgg tcgggttcgt catgttcctg
     3661 gtgctgaact tcggggggct cctgacctgg gtcgagcgca agcagtccgc gatcatgcag
     3721 gaccgcatcg gggccaaccg ggcgtccatc tcggtgctcg ggttccgcat ccggatcctg
     3781 ggcctgctcc acccgctcgc cgacgcggtg aagatgctca ccaaggagga cttcaccccc
     3841 gcgcgcgcgg acgtcctgct gttcaagctg gcccccttcg tgtcggtgtt cttcggcctc
     3901 gccgccttcg cgtcgatccc gttcggcgac acgctgcaga tcatggggcg cgagatccag
     3961 ctccaggcgg tgaccctcaa cgtgggcatc ctctacgtgc tggccatgat ctcgctcggg
     4021 gtctacgggc tgatgatggc gggctgggcc tcggccaaca actacgccct cctgggcggg
     4081 cagcgcgcgg cggccctcat gatctcggcc gagatcgcga tcggggcctc gatcatgggc
     4141 gtggtgatgg tctacggctc gctcaacatg caggacatcg cgcgtgggca gggccagccg
     4201 ctgctgccgc aattcctcgg caactggatc ccggcctggg gcgtgctcac ccagcccgcc
     4261 gcgttcatcc tgttcctcac cgcgggcatc gcctccacca agcgcatccc cttcgacacc
     4321 cccgagggcg agtcggagat catcggctac ttcgtcgagt acagcgggat gaagttcggc
     4381 atgttcgcga tggccgactt cctcgagacg gtggtgatcg cggggatgac caccgcgctg
     4441 ttcctgggcg gctggcaggt gccgtacctc caggcctccg gcttcgtctt cccgtggggc
     4501 gcgagcgtgg ccctgccgca cctcgcggtg gtcggcctcc aggtcgtgtc cttcctcacc
     4561 aaggtcgtgg tcatgctctg gtttctcatg ctcatccgct ggacgctccc gcggtttcgc
     4621 tacgaccagg cgatgcggct cggctggctc gccctcttcc ccctgtcggt cctcaacatt
     4681 ctcgtcaccg cgctggtgct gctcgcggtg gggaagggct gagccgtggc gtaccagacc
     4741 gagaacaccc ccgccgatcc gcggcactcg agccggctcg gcctccgcca gcgcttctac
     4801 gtggtggagg tgctggtcgg gctcgcgctg acctcccagc acttcttcgc caacatgtgg
     4861 cgccacacgt tgaaggtcgt gttccgcagc aagaccgccc gcggcgcggt caccatccag
     4921 tacccggacg agcggcggcc ctactcgccc cggctgcgca gcctgcaccg gctggtgcgc
     4981 cgggaggacg gctcgccgcg ctgcgtggcc tgcatgatgt gcgagacggt gtgcccggcc
     5041 cactgcatct acatcgtggc caccgagcat ccgaatccgg agatcgagaa ggtcccggag
     5101 cggttcgaca tcgacctcgg caagtgcgtc ttctgcggct tctgcgtcga ggcctgcccc
     5161 gaggacgcca tccggatgga caccggcatc ctcgagttcg cggcctacga ccgggcgagc
     5221 atggtctaca ccaaggacac gctgctggcg ctcgaggcgg ccggcgcgga cggcgtcccc
     5281 acctcgccgg tcccgattcc cccgtcgcgg agaccggcgt gagcttcgtc accttcttcg
     5341 tcctggccgc cctcgcggtc ggctcggcgg cggggctgct cctgcgccgg aacccgatcc
     5401 acggggccct gttcctcgtg gtcaacctgg gcacgatcgc ggccctgttc ctgactttgc
     5461 gggccgagtt cctggccgcg gtgcaggtga tcgtctacgc gggcgccatc gcggtgctct
     5521 tcgtgttcgc gatcatggtg ctgatccccg gcaaggagga gaccgggccc gatccgctgc
     5581 gcggccagcg cctgctgtcg gtgccggtgg tgggcgtctt cctggccctg gtcgcggtgg
     5641 tcgtcggctc ggccaccctc gcgggcagcc cgaagccgcc ggtggccgga ggcgcggacg
     5701 cggtggggcg cgtgctcttc accgactacc tgtttccctt cgaggtcacc tcggtgctgc
     5761 tgctggccgc catcgtgggc gtgatggcgc tgaccaagcg caaggcctag gcccgctgat
     5821 ggtgcccgag tcctactacg tcgcgctgtc cgccgtgctc ttcagcatgg gcgtgctcgg
     5881 cgtgctgatc cggcgaaacc cgctggtgat cttcatgtcg atcgagctga tgctcaacgc
     5941 ggcgaacctg gccctggtcg ccttcggcca gcgccacggg acgaccgagg gacaggctct
     6001 ggtattcttc gtgatgtgcg tggccgccgc cgaggtcgcg gtgggcctcg cgatcatcgt
     6061 ggcgatcttc cgccagcgcc ggcgcctctc ggtcgacgag ctgagcaccc tccgagggta
     6121 gccaagaagc atacggtagc agcggcaacg caacgttcca ggaggagacc gatggcgcag
     6181 acgaaaaccg caccgccccc gacccgcctc gacggcgagc tggcgcggcg gctgctgacc
     6241 cagatggcgc tgatccggcg cttcgaggag aaggcggccg agatgtacgc cctcggcaag
     6301 gtcggcggct tcctccacct ctacatcggg caggaggcgg tcgcggtcgg cgccacctcg
     6361 atctgccgcc ccgacgacta cgcggtgtcg tcctatcgcg agcacggcca ctgcctggcc
     6421 aagggctcgg acccgcgccg catgatggcc gagctgttcg ggcgcatggg cgggctgagc
     6481 cacggcaagg gcggctccat gcatctcttc gacaagagcg tcaacttcct gggcggccac
     6541 ggcatcgtgg gcgcgcacct gccgctcgcg gcgggggcgg gcttcgcgat caagtaccag
     6601 gggggcgacc aggtggtgct gtgctacttc ggcgacgggg cggtgccgga gggcgagttc
     6661 cacgagtcga tgaacctggc cgcgctgtgg aagctgccgg tgatcttcat ctgcgagaac
     6721 aaccgctacg cgatgggcac cgcgatccac cgcgcgctgg cccagaccga gatctggcgc
     6781 ttcgcggaga cctacggcat tcccggcgag gcggtcgacg gcatggacgt gctggcggtc
     6841 cgggacacgg tggggcgggc ggtcgagcgg gcgcggcgcg acaagacccc cacgctggtg
     6901 gaggcgcgca cctatcgctt ccgcggccac tcgatgcgcg acccggccgg ctcggtctac
     6961 cggaccaagg aggaggtcga gcgcgagaag ctgcgggacc ccatcgcgct cttcaccgat
     7021 cgctgcctca aggagggcgc cctgaccgag gccgacctca agaacatcga gaaggcggtc
     7081 aacgacctgg ccgacgaggc ggtcgcgttc gcggaggcct cccccgaacc gccgcccgaa
     7141 gagctgttca ccgacgtgta caaggactga cgccatgtcg gtgatgacct atcgcgaggc
     7201 cctcaacgtc gccctccgcg aggagatgcg ccgggacccg cgcgtgttcg tgatgggcga
     7261 ggaggtcggt ctctacgacg gcgcctacaa ggtcacccag gggctgctca aggagttcgg
     7321 cgacaagcgc atcatcgaca cgcccatctg cgaatcgggc ttcaccgggg tcggggtcgg
     7381 ggcggcgatg ctcgggctgc ggccggtgat cgagatgatg acgttcaact tctcgatcct
     7441 ggccctggac cagatcgtca actcggcggc caagatgtgc tacatgtccg gcggccagta
     7501 caacatcccc ctcgtggtcc gcggtccggg cgggccggcg gcacagctcg cggcgcagca
     7561 ctcccagtcg atggagacgt acttctacca cgtgcccggg ctcaaggtgg tgcggccgtc
     7621 gacgccggcc gacgccaagg gcctgctcaa gtccgccatc cgcgacgaca atcccgtcat
     7681 cttcatcgag gcggagacgt tgtacgcggt gaagggggag gtgccggacg atccggactt
     7741 cctgattccc ctcggccagg cggtgatccg tcgcgagggc accgacgtca cggtggtggc
     7801 ctacatgggc atgctctacc gggcgctcga ggtcgccgag gagctcgcca aggagggcat
     7861 ctcggtcgag atcgtcgacc cccgcaccct ccggccgatg gacgccgaca ccatcatcgg
     7921 ctcggtgcgg aagacgcacc gggccgtcgt gctcgaggcg ggcgcgggct tcgcggggat
     7981 gggctcggag atcgcggccc agatcaccga gaacgccttc gacgatctcg acgcgcccgt
     8041 cgagcgggtc accggcgaga acgccccgat gccgtacgcc aagaacctcg agctgctgaa
     8101 gacgccgtcc aaggccaaga tcgcggcggc catccgccgg gtgtgtaacc gatgatcacc
     8161 aaggtgatca tgcccaagct gtcggacgcc atggagaccg gcaaggtcat caagtggctc
     8221 aagaaggagg gcgaggcggt caagggggga gacatcctcg tcgaggtcga gaccgacaag
     8281 gccaacgtcg aggtcgaggc gttcggcagc ggcgtgctgc gcaagatcgt gatcgccgag
     8341 ggcgaccagg tgccggtcgg cgatctgatc gccgtgatcg cggacgcggc ggaggacatc
     8401 tcgagtgtcg ccgccacccc ggccaagccc gcgcccccgg ctccggcggc cccggccgcc
     8461 gccgcgccgg ccgccccggc gccggctccg cgtcccgc
//
LOCUS       DATMCV010000214        15043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_808532, whole genome shotgun sequence.
ACCESSION   DATMCV010000214 DATMCV010000000
VERSION     DATMCV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 15043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15043
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808532"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /gene="lepA"
                     /locus_tag="VNU03_05015"
     CDS             complement(<1..1486)
                     /gene="lepA"
                     /locus_tag="VNU03_05015"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWP73848.1"
                     /translation="MAIDLIRNFSIVAHIDHGKSTLADRLLSLTGTMDAKKAVDQVLD
                     SMDLEKERGITIKAHTVRLLYKARDGQEYTLNLIDTPGHVDFSYEVSRSLAACEGALL
                     IVDAAQGVEAQTLANFYLALDGGLEIIPVINKIDLPAADLEGTRSQITEILTLDGDEA
                     LAISAKHGTGVPEVLEAIVARIPPPKGDPDAPLKALVFDSFYDSYQGVVVYVRLLDGR
                     VHAGDKILLMSNGKNYEVQQVGVFSPDMRPVEELSAGQVGYLTASIKRVADAKMGETI
                     TDVLHPTAAPCPGYRDAKPMVFAGLYPIEDTDYEGLRDALEKLRLNDSAFNFEPETSL
                     ALGYGFRCGFLGMLHLEIVQERLEREFNLSLIVTAPSVRFKVITMAGETLEVENPSKL
                     PPVDRIERIEEPYVRGSVFVPTEFMSEIYKLAQEKRGEHRSLEYVGKRVHIQFDFPLS
                     EIVVDFYDRLKSISKGYASFDYEFLEFRASDMVKLDILLNGDPVD"
     gene            complement(1598..2734)
                     /locus_tag="VNU03_05020"
     CDS             complement(1598..2734)
                     /locus_tag="VNU03_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HWP73849.1"
                     /translation="MPEGVPDPGRHSRDADRRGGALGPDEITAVILAGGQGTRLRPLT
                     LGTPKPIVPLLNVPFLAYQLALLRRHGIRDVVLSCSYLVDEVRRAMGDGAAHGVRLSY
                     AVETDPLGTAGGVRNAVDLVRGLVVVLNGDILADLDLSAMLRFHRERGARATLYLTRV
                     SDPTQYGLVELEPDGAIRAFVEKPEPDRVNTDTVNAGVYALDLSVVTAIPTGRAVSIE
                     RETFPGLLRDRVPFFGWVSESYWLDIGSPAKYRQGQLDLLAGKVATDVAPPGDARARR
                     FVAPGAHVAPDAIVAAPCVIGPGSRLEAGARVGPNAVLGANCVVGAGAHVEGAILWDD
                     VTVGPGAVLRDCIVASGARIGAGADLGPAAVLGAGTSVPDRARL"
     gene            complement(2653..2823)
                     /locus_tag="VNU03_05025"
     CDS             complement(2653..2823)
                     /locus_tag="VNU03_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HWP73850.1"
                     /translation="MIDEELRAILACPACKGELIFEETRIICPACRKAYPIRDGIPVM
                     LIGEAEPWAPTR"
     gene            complement(2820..3749)
                     /locus_tag="VNU03_05030"
     CDS             complement(2820..3749)
                     /locus_tag="VNU03_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWP73851.1"
                     /translation="MKILVTGGAGFIGSHVVDRFVEAGHDVCVVDNLTTGDARWVNPR
                     ARLHVVDLRNARLAEVFEKERPEVVAHLAAQASVGRSVTDPIFDASVNVVGGLGLLEC
                     CRRFGVRRIIYSSSGGAGYGDTDVIPTPETHPTVPISPYGITKVAMEYYVNAWSGIFG
                     ISGISLRYANIYGPRQNPQGEAGVIAIFCHRLLTSQSPIINGDGDQTRDYTYVEDVAL
                     ANLRALERPDVTGAVNVCTGVETTVNQLYTALVAASGAKVPAQHAPARPGEQRRSCLS
                     PARAEQVLGWKPSVSLAEGLGRTFHFFQKEVSA"
     gene            complement(3746..4681)
                     /locus_tag="VNU03_05035"
     CDS             complement(3746..4681)
                     /locus_tag="VNU03_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HWP73852.1"
                     /translation="MRVLVTGGAGFIGSHLCDFLLARGCEVVCMDNLLTGSTDNIAHI
                     SDSRFLFVKHDVTNYIVVGGPLDYVLHFASPASPIDYLELPIQTLKVGALGTHKALGL
                     AKERRAKFLLASTSEVYGDPLVHPQREDYWGNVNPVGPRGVYDEAKRFAEAMTMAYHR
                     VHGLDARIVRIFNTYGPRMRLTDGRAIPTFVRQALRNEPITVFGDGSQTRSFTYVSDL
                     VEGIWALMQAPINEPVNIGNPREMTLLQLARQIIQMSGSRSEVVFRPLPTDDPKVRQP
                     DITKARRVLGWEPTIEVEDGLRRTIDWCRKLVTQP"
     gene            complement(4695..6005)
                     /locus_tag="VNU03_05040"
     CDS             complement(4695..6005)
                     /locus_tag="VNU03_05040"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWP73853.1"
                     /translation="MNICVVGTGYVGLVTGAVFADLGNDVICVDNALKKIEALRAGQM
                     PIYEPGLEEMVARNKDDGRLTFSTDLPAGVRQADVIFIAVGTPPKETGETDLSHVEAV
                     AAEIGRSMDRYKVVVNKSTVPVGTGELVREVIMRHQPRPLDFDVVSNPEFLREGSAIE
                     DTLRPDRIVIGAPNQQVAMTLVELYAPLERPMIITDLPSAEVIKYASNSFLAAKISFI
                     NAIANICEAAGADVSQVMKGMGLDSRIGMQFLQAGLGYGGSCFPKDVDSLIHTASQLG
                     YDFKLLRSVVEINKERAAHLVEMMRKAMGPLEDKTIAVLGLAFKPNTDDMREAKSLEV
                     VRALHAAGAQVRAYDPVAMENARGLMPEGVVFCESAYEAAAGADGVTLLTEWNEFKYL
                     NLDRLRGLLRRRLIFDARNLYEPERMRRLGFEYYSIGRKPVLAG"
     gene            complement(6081..7016)
                     /gene="cyoE"
                     /locus_tag="VNU03_05045"
     CDS             complement(6081..7016)
                     /gene="cyoE"
                     /locus_tag="VNU03_05045"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWP73854.1"
                     /translation="MPRAAAASSRGLPALGVAARVSAYAELSKFGIVLLVLVSAGAGF
                     MLRAPLGPDFPWAQGLLVLAGVMLLSCGASALNQVQERSRDALMARTAGRPLPSGRLT
                     HAQCLAFAVLGIAAGSALLWLGVGRTAGILGLIASAFYNGVYTPWLKTHSPFAAVPGA
                     VPGAIPPVIGWVAGGGSVTDAGAVILFGILFLWQMPHFWALALRYRGDYAAGGFPMVS
                     ERVGVEGTARLILLYALGLTAWSLAAPTFGVGGAAAFVAAGALGGRLIWLAEKFARQP
                     DDPRGWLSLFLFSNFYLLLLFLVMVGERLIRHTLS"
     gene            complement(7009..7896)
                     /locus_tag="VNU03_05050"
     CDS             complement(7009..7896)
                     /locus_tag="VNU03_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HWP73855.1"
                     /translation="MSAKIALAAVAAVFVLMTLGNVVSATGSGLACPDWPLCHGSVIP
                     PLRADVLIEYSHRLSALAATVLILATSVVTLRGAAPAGLRRLAWLLPILLVVQIALGA
                     ITVLLKLPDLVSTTHLVNALLILGGLIVLALGLRGPARPPAAGAERTRRLARVGLAVL
                     LVQLALGGYVRHAGAGLACPDFPLCSGDLLPSGWLASVHWVHRWLGVLLLGVFVHLAV
                     AGRSTATRLVAVLAVLQVSLGVATVLLQLAPPIRAAHAAVGYALWATLVWASVEAGCW
                     RAASAPTSPPAATRKVAHA"
     gene            complement(7886..8755)
                     /locus_tag="VNU03_05055"
     CDS             complement(7886..8755)
                     /locus_tag="VNU03_05055"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWP73856.1"
                     /translation="MAHVVPGPAGPPPLGPDGFDWTAWSPDPSVVGGLLALGVVYAAA
                     TLWRHRLDPEARVEPAKVVSFAGALAVLLGALTGPVHDLSDYYLFSAHMIQHMLLVFA
                     MPPLLLYGTPGWMARPLLRDARLLQLGRALTRPVGAFATFNVVLVAWHLPPLYNLAME
                     QHPVHIVEHLMIMVASVILWWPMLSPLEELPRAPYPVQMLYLFVVGLPMVMVAIFISM
                     ADSLLYPYYAAAPRIWARLTPQADQHLGGLIMWIPGGMIFLAALSVVFFRWQAAGGDD
                     GAVHPTRTATRVG"
     gene            complement(8757..9101)
                     /locus_tag="VNU03_05060"
     CDS             complement(8757..9101)
                     /locus_tag="VNU03_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWP73857.1"
                     /translation="MADQHAVAAATHGGGGGHATVKTYINVAIVLAIITAVEVASLYI
                     PGIPGSVLVISLLAMSAVKFFLVVGFFMHLRYDHPIMRALFVGPLLIAIVIILAVMAL
                     FGAFVLLPRVVL"
     gene            complement(9112..9717)
                     /locus_tag="VNU03_05065"
     CDS             complement(9112..9717)
                     /locus_tag="VNU03_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWP73858.1"
                     /translation="MADHAAVHAVHDPNIGNTGLDNRKVGMWAFLGSDCMFFGSLIGT
                     YLAYKGKSISGPLPHEVFDIPLTSYSAALLLASSLTMVLALAALQQNRIKPATRWLFV
                     TAMLGLHFVLNQVYEFTSFVLHHNLTLSTSLFGSSFFVLTGFHGAHVTGGVIWLLILV
                     MLGMRGKLDARDYTKVEIAGLYWHFVDIVWIAIFTVIYLIP"
     gene            complement(9728..11593)
                     /gene="ctaD"
                     /locus_tag="VNU03_05070"
     CDS             complement(9728..11593)
                     /gene="ctaD"
                     /locus_tag="VNU03_05070"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HWP73859.1"
                     /translation="MATQAAFMHGSKVETETFWGIVWSWLTTVDHKRIGILYGTSAFI
                     FFLIGGIEALIMRLQLARPDNTLVGPERFNELFTMHGTTMIFLAVMPLSSAFFNYMIP
                     LMIGARDVAFPRLNALSYWIFLSGALFLNSSWLFGLAPDGGWFGYANLTSIQFSPKHT
                     IDFWMLGLQILGLSSMLAGFNFLVTIINMRAPGMTLMRMPVFIWMSFVTQFLLVLAFP
                     AITVALILLMFDRFFGTLFYAPAAGADPLLWQHLFWIFGHPEVYILILPAMGIVSEVL
                     PTFSRKPLFGAPVVIYSGIMIGFFGFGVWSHHMFSVGMGPVADAAFSISTMLIAVPTG
                     VKILNWIGTLWGGSIRFRTPLYFSLGFIAMFTMGGLSGIMHASPPVDLQQTDTYFVVA
                     HFHYVLFGGSIFGLFSGIYYWWPKITGKLLDERLGLWHFWLMMLGFNMTFFPQHYLGL
                     IGMPRRIYTYAPDLGWNFWNMVSTIGAFLIAVSLLFFIANVIRTMKGGQVAGNDPWDA
                     RTLEWAIPSPPPVYNFARVPQVQDRDDFWLAKHGDGHGSAPRPKPAPVTAAEIAAIHM
                     PPTSYWPLFLGLALAAMVTGLMISMYQVAIGGLLTLYLMYKFAMELHRPPAGHGH"
     gene            complement(11603..12652)
                     /gene="coxB"
                     /locus_tag="VNU03_05075"
     CDS             complement(11603..12652)
                     /gene="coxB"
                     /locus_tag="VNU03_05075"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWP73860.1"
                     /translation="MMKPPATIPRPRRRFDWTQACLLGAVLLLASSCSAVGWNWDTPM
                     STVMPKSDFGKMTHEIFMLISWWTLGIFIVVEVGLAYVCWRFRDRAGAPIPKQVHGHT
                     ALEVGWTIAFAVILLVFAIPTIRVIFKTQEAPAATALRVEVFGKQWWWEFKYPQFKIT
                     TANELHLPVGQTATFLLNGPDVIHSFWMPQLGGKRDVVPHRINHITLTPEVAGDYPGQ
                     CAEYCGISHANMRFRVIVHEPADFEKWVKAQQAPPVESADALAQQGKTIFGQSTCVGC
                     HTITGVSAGHIGPDLTHFASRKRFAGSLMESTPDNIAKWVENPDHMKPGALMPNLGMT
                     GEQSKAIAAYLLSLK"
     gene            complement(12670..12936)
                     /locus_tag="VNU03_05080"
     CDS             complement(12670..12936)
                     /locus_tag="VNU03_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73861.1"
                     /translation="MPTIVHLLLALVLTVFLVAQAKSLAFVLRSRRAVSSRSARRGTD
                     LLWTCIPVLLVLFLAARSWVAVVDVHRPAVAATGAPAQSPVTPR"
     gene            complement(13052..14284)
                     /locus_tag="VNU03_05085"
     CDS             complement(13052..14284)
                     /locus_tag="VNU03_05085"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HWP73862.1"
                     /translation="MATSSITLARLKDHIETLSTYGRNADGHGITRSCWSPVHEQARA
                     WLLSRMKAAGLQTRVDAAGNTFGMLPGEGAAVLTGSHIDTVPEGGPLDGALGVLAGLE
                     CLQTVKEVGLPVRRPLAVVAWSDEEGRYGSLFGSRAFTGRLDLAQITTLRSLDGESLV
                     EAMARAGYDASRVAEARCDPRTLHAYVELHIEQGPHLEQARIPIGLVEGIVGIRRNRL
                     TFIGEPDHAGTTPMARRKDAFLAACEYALKAREHIVRKGSGRSVTNFGRIELAPGVSN
                     IVPARAVLMQEMRELDARILARLDRECVTLARTVARRRGLRVVVDTLSRTDPARCAPR
                     VMDAVQAACDALRLRTRRMPSGAGHDAQNLAGVTASGMLFIPSRGGRSHRPDEMSDWK
                     AIGRGTNALLQTLLRLAG"
     gene            14440..>15043
                     /locus_tag="VNU03_05090"
     CDS             14440..>15043
                     /locus_tag="VNU03_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73863.1"
                     /translation="MDFTLTPEQESFRGEVRAWLSKQMRQEWVARLSKGSDIPRPEAY
                     ELLRRWQGQLHEAGFVGLTWPKEAGGRGLTFMEEMILQQEMALSKAPPVLNILAIGMA
                     GPTIIAYGTDEQKRRYPPKMLSCEEIWCQGYSEPNAGSDLASLQTRAVKDGDHYVVNG
                     QKVWTSLAHIADYMMLLARTDPSAPKHKGITYFLLDMKSPG"
BASE COUNT         2468 a         4992 c         5495 g         2088 t
ORIGIN      
        1 cgtccaccgg gtcgccgttg aggaggatgt cgagcttgac catgtccgag gcccggaact
       61 ccaggaactc gtagtcgaag gacgcgtagc ccttcgagat ggacttgaga cggtcgtaga
      121 agtccaccac gatctcgctc aaggggaagt cgaactggat gtgcacccgc ttgcccacgt
      181 actcgagcga ccggtgctcg ccccgcttct cctgggcgag cttgtagatc tcgctcatga
      241 actcggtggg cacgaagacg gagccgcgca cgtagggctc ctcgatgcgc tcgatgcggt
      301 cgacgggcgg cagcttggag gggttctcga cctccagcgt ctcccccgcc atcgtgatca
      361 ccttgaagcg cacgctcggc gcggtgacga tcagggagag gttgaactcc cgctcgagcc
      421 gctcctgcac gatctcgagg tgcagcatgc cgaggaaccc gcagcggaac ccgtaaccaa
      481 gcgcgagtga agtctccggt tcaaaattga acgcggagtc gttcaggcgc agcttctcca
      541 gcgcgtcgcg caggccctcg tagtcggtgt cctcgatcgg gtagaggccc gcgaacacca
      601 tgggcttcgc gtcgcggtag ccgggacagg gcgccgcagt cgggtgcagg acgtcggtga
      661 tggtctcgcc catcttggcg tcggccaccc gcttgatgga ggcggtcagg tagccgacct
      721 gcccggccga cagctcctcg accggccgca tgtccggcga gaagacgccg acctgctgga
      781 cttcatagtt cttgccgttg gacatcagga ggatcttgtc cccggcgtgc acccgcccgt
      841 cgaggagccg gacgtagacc accactccct ggtaggagtc gtagaaggag tcgaagacga
      901 gggccttgag gggggcgtcc ggatcgccct tgggcggcgg gatccgcgcc acgatcgcct
      961 ccaggacctc cggcacgccg gtgccgtgct tggccgagat ggccagggcc tcgtcgccgt
     1021 ccagggtgag gatctcggtg atctggctgc gggtgccctc caggtcggcg gcgggcaggt
     1081 cgatcttgtt gatgaccggg atgatctcga ggccgccgtc gagcgccagg tagaagttgg
     1141 ccagggtctg ggcctcgacg ccctgggccg cgtcgacgat cagcagggcc ccctcgcacg
     1201 cggccagcga ccgggagacc tcgtaggaga agtccacgtg gccgggggta tcgatcaggt
     1261 tgagggtgta ttcttgcccg tcccgcgcct tgtagagcag gcgaacggtg tgggccttga
     1321 tggtgatgcc gcgctccttc tccaggtcca tcgaatccag gacctggtcc accgctttct
     1381 tggcgtccat ggtcccggtc agcgatagca ggcggtccgc cagggtggac ttgccgtggt
     1441 cgatgtgcgc gacgatggag aaattgcgga tcaggtcgat ggccatccgg ctaagaatac
     1501 cacgccgttc ggccaccctg cggccgggcg gcgacgggcc gccctcccgt ctgcgagggg
     1561 agcccggatc gggcgcggcg gaggccgccg aggggcccta gagccgggcc cgatcgggga
     1621 cggaggttcc cgcgccgagc accgcggccg gaccgaggtc ggccccggcg ccgatccgcg
     1681 cccccgaggc cacgatgcag tcgcgcagga ccgcgcccgg cccgacggtc acgtcgtccc
     1741 acaggatggc gccctcgacg tgcgccccgg ctccgaccac gcagttcgcc ccgaggaccg
     1801 cgttcgggcc cacccgggcc cccgcctcga ggcgcgagcc gggcccgatc acgcacgggg
     1861 ccgcgacgat cgcgtcgggg gcgacgtgtg ccccgggggc gacgaagcga cgcgcccggg
     1921 catcgccggg tggggccacg tcggtcgcga ccttgccggc cagcaggtcg agctggccct
     1981 gccggtactt ggcggggctg ccgatgtcga gccagtagct ctccgacacc cagccgaaga
     2041 agggcacccg gtcgcgcagg aggccgggaa acgtctcgcg ctcgatcgag accgcgcggc
     2101 cggtggggat cgcggtcacc accgagaggt cgagcgcgta gacccccgcg ttgacggtgt
     2161 cggtgttcac ccgatccggc tcgggcttct ccacgaaggc gcggatcgcc ccgtcgggct
     2221 cgagctcgac gaggccgtac tgggtagggt cggacacgcg cgtcaggtag agggtcgcgc
     2281 gcgcgccgcg ctcccggtgg aagcgcagca tcgcggagag gtcgaggtcg gccaggatgt
     2341 cgccgttgag caccaccacg agcccgcgca ccaggtccac cgcgttgcgg accccgcccg
     2401 cggtcccgag gggatcggtc tcgaccgcgt aggagagtcg caccccgtgg gcggcgccgt
     2461 cgcccatggc ccggcgcacc tcgtccacca ggtacgagca cgagagcacc acgtcgcgga
     2521 tgccgtgacg ccggagcagc gcgagctggt aggcgaggaa ggggacgttg aggagcggga
     2581 cgatcggctt cggggtgccg agggtcagcg ggcgcagtcg cgtgccctgc ccgccggcca
     2641 ggatgaccgc ggttatctcg tcggggccca gggctccgcc tcgccgatca gcatcacggg
     2701 aatgccgtcc cggatcgggt acgccttccg gcaggccggg cagatgatgc gcgtctcctc
     2761 gaagatcagc tcgcccttgc acgccgggca ggccaggatc gcccgcagct cctcgtcgat
     2821 catgccgaga cctccttctg gaagaagtgg aaggtgcggc cgaggccttc cgcgagcgac
     2881 acggagggct tccagccgag gacctgctcg gcccgcgccg ggctcaggca gctgcgccgc
     2941 tgctcgccgg gccgcgcggg cgcgtgctgg gcggggacct tggcgccgga ggcggcaacc
     3001 agggcggtgt agagctggtt cacggtggtc tcgacgccgg tgcacacgtt gaccgccccg
     3061 gtgacgtcgg gccgctcgag cgcgcgcagg ttcgccaggg ccacgtcctc cacgtaggtg
     3121 tagtcgcggg tctggtcgcc gtcgccgttg atgatcggcg actggctggt gagcagccgg
     3181 tggcagaaga tcgcgatgac gccggcctcg ccctgcgggt tctgccgcgg cccgtagatg
     3241 ttggcgtagc ggagcgagat gccgctgatc ccgaagatgc cgctccaggc gttgacgtag
     3301 tactccatcg cgaccttggt gatcccgtag ggcgagatcg gcaccgtcgg gtgcgtctcc
     3361 ggggtcggga tcacgtcggt gtcgccgtag ccggcgcccc cgctcgacga gtagatgatc
     3421 cggcgcaccc cgaagcggcg gcagcactcg agcagcccca ggccccccac cacgttcacg
     3481 ctcgcgtcga agatgggatc ggtgaccgag cggcccaccg aggcctgggc ggccaggtgc
     3541 gcgaccacct ccggccgctc cttctcgaac acctccgcga gccgcgcgtt ccggaggtcg
     3601 accacgtgca ggcgggcgcg ggggttgacc cagcgcgcgt cgccggtggt gaggttgtcg
     3661 accacgcaga cgtcgtggcc cgcctcgacg aagcggtcca ccacgtggga gccgatgaag
     3721 ccggcgccgc cggtgacgag gatcttcatg gctgggtcac cagctttcgg caccagtcga
     3781 tcgtgcggcg cagaccgtcc tcgacctcga tcgtgggctc ccaccccagc acgcggcgcg
     3841 ccttggtgat gtcgggctgc cgcaccttcg ggtcgtcggt gggcaggggg cggaacacga
     3901 cctcgctgcg ggagccgctc atctggatga tctggcgcgc gagctggagc agggtcatct
     3961 cccgcgggtt tccgatgttg accggctcgt tgatcggggc ctgcatgagg gcccagatgc
     4021 cctccaccag gtcggacacg taggtgaacg agcgggtctg ggagccgtcg ccgaacacgg
     4081 tgatcggctc gttccgcagc gcctggcgga cgaaggtggg gatggcccga ccgtcggtga
     4141 gccgcatgcg cgggccgtag gtgttgaaga tgcgcacgat gcgggcgtcc aggccgtgca
     4201 cgcggtggta ggccatggtc atggcctccg cgaaccgctt ggcctcgtcg tagaccccgc
     4261 gcgggcccac cgggttgacg ttgccccagt agtcctcgcg ctgcgggtgc acgagcggat
     4321 cgccgtagac ctcggaggtc gacgcgagca ggaacttggc ccggcgctcc ttggccagcc
     4381 cgagcgcctt gtgggtcccg agggcgccga ccttgagggt ctggatcggc agctccaggt
     4441 agtcgatcgg ggacgcgggg ctcgcgaagt gcaggacgta gtcgagcggt ccgcccacca
     4501 cgatgtagtt ggtgacgtcg tgcttgacga agaggaatcg cgagtcgctg atgtgggcga
     4561 tgttgtcggt cgacccggtc aggaggttgt ccatgcagac cacctcgcag ccgcgcgcca
     4621 ggaggaagtc gcacaggtgg gagccgatga agccggcccc gcccgtcacc agcactcgca
     4681 tcgggctcgg ctcctcagcc ggccaggacg ggcttgcgcc cgatggagta gtactcgaag
     4741 ccgagtcgcc gcatgcgctc gggctcgtag agattgcggg cgtcgaagat cagtcgacgg
     4801 cgcagcaggc cccggagccg gtccaggttc aggtacttga actcgttcca ctcggtgagc
     4861 agggtcaccc cgtcggcccc cgcggccgcc tcgtaggccg actcgcagaa caccacgccc
     4921 tccggcatga ggccccgcgc gttctccatc gccaccggat cgtaggcgcg gacctgagcc
     4981 cccgccgcgt gcagcgcccg caccacctcg aggctcttgg cctcgcgcat gtcgtcggtg
     5041 ttgggcttga aggcgagccc caggaccgcg atcgtcttgt cctcgagcgg ccccatcgcc
     5101 ttgcgcatca tctccaccag gtgcgcggcg cgctccttgt tgatctccac caccgagcgc
     5161 agcagcttga agtcgtaacc gagctgcgac gcggtgtgga tgagcgagtc cacgtccttg
     5221 gggaagcagg agccgccgta gccgaggccg gcctggagga actgcatgcc gatgcgggag
     5281 tcgaggccca tgcccttcat cacctggctc acgtcggcgc ccgccgcctc gcagatgttg
     5341 gcgatcgcgt tgatgaacga gatcttggcg gccaggaacg agttggaggc gtacttgatc
     5401 acctccgccg agggcaggtc ggtgatgatc atcggccgct cgagcggggc gtacagctcg
     5461 acgagggtca tggccacctg ctggttgggg gcgccgatca cgatgcgatc gggccggagc
     5521 gtgtcctcga tcgcggagcc ctcgcgcagg aactcggggt tggacaccac gtcgaagtcg
     5581 agcgggcgcg gctggtggcg catgatcacc tcgcgcacga gctcgcccgt cccgaccggc
     5641 accgtcgact tgttgaccac caccttgtag cggtccatgg agcggccgat ctcggccgcg
     5701 accgcctcca cgtgcgagag gtcggtctcg cccgtctcct tgggcggggt gccgaccgcg
     5761 atgaagatca cgtccgcctg gcggaccccc gcgggcaggt cggtggagaa ggtgagccgg
     5821 ccgtcgtcct tgttccgggc caccatctcc tcgaggcccg gctcgtagat cggcatctgg
     5881 ccggcccgga gcgcctcgat cttcttgagc gcgttgtcca cacagatcac gtcgttgccg
     5941 agatcggcga agacggcgcc ggtcacgagc ccaacgtaac ccgttcccac cacgcaaatg
     6001 ttcatcggtc acgcccctcc agacggaatc tcaggatagc agacgaccct cccaccgcgc
     6061 caaccccggg gccggccccc tcaggacagg gtgtggcgga tcaggcgctc gcccaccatg
     6121 accaggaaca gcaggagcag gtagaaattc gagaagagga agagcgacag ccacccccgc
     6181 ggatcgtccg gctggcgcgc gaacttctcg gccagccaga tcaggcgccc gccgagcgct
     6241 cccgccgcca cgaaggcggc cgcgccgccg accccgaacg tgggggcggc cagcgaccag
     6301 gcggtgaggc cgagggcgta gagcaggatg aggcgggcgg tgccctccac cccgacccgc
     6361 tcggacacca tcgggaaccc gcccgccgcg tagtcgccgc ggtagcggag ggcgagggcc
     6421 cagaagtgcg gcatctgcca gaggaagagg atgccgaaga ggatcaccgc ccccgcgtca
     6481 gtgaccgagc cgccgcccgc cacccacccg atgacggggg gaatcgcccc cggcaccgcg
     6541 ccgggcaccg ccgcgaacgg cgagtgcgtc ttgagccacg gagtatagac cccgttgtag
     6601 aaggcgctcg cgatcaggcc caagatcccc gcggtgcgtc ccaccccgag ccacagtaga
     6661 gccgagccgg ccgcgatgcc gaggacggcg aacgccaggc actgggcgtg ggtgagccgg
     6721 ccggagggca gcggccggcc cgcggtgcgc gccatgagcg cgtcgcgcga gcgctcctgg
     6781 acctgattca gcgcggaagc gccgcaggag aggagcatca cgccggccag caccagcagg
     6841 ccctgcgccc acggaaagtc ggggccgagc ggcgcgcgaa gcatgaagcc cgcccccgcc
     6901 gagaccagca ccagcagcac gatgccgaac ttggagagct ccgcgtacgc gctcacgcgc
     6961 gcggccacgc cgagggcggg gagcccgcgc gaggacgccg cggcggccct aggcatgggc
     7021 caccttcctc gtggcggccg ggggcgacgt cggggcgctc gcggcccgcc agcagccggc
     7081 ctcgacgctg gcccacacca gcgtggccca cagcgcgtag ccgaccgccg cgtgcgcggc
     7141 ccggatgggc ggggccagct gcagcagcac ggtcgcgacg ccgagcgaga cctgcagcac
     7201 cgcgagcacg gcgacgaggc gcgtcgcggt gctgcgcccg gccaccgcca ggtggacgaa
     7261 gacgccgagg aggagcacgc cgagccagcg gtggacccag tgcaccgagg ccagccaccc
     7321 gctcggcagc aggtcgccgc tgcagagcgg gaagtccggg caggcgagcc ccgcgcccgc
     7381 gtgccgcacg tagccgccca gggccagctg gaccaggagc accgcgaggc cgacccgggc
     7441 caggcggcgc gtccgctcgg cgccggccgc gggtggccgc gcggggccgc gcagcccgag
     7501 ggcgagcacg atgagaccgc ccaggatcag cagagcgttc accaggtgcg tggtgctcac
     7561 caggtccggg agcttcagca gcaccgtgat cgccccgagg gcgatctgga ccacgagcag
     7621 gatcgggagg agccacgcga gccgccggag cccggccggc gcggccccgc gcagcgtgac
     7681 cacgctcgtg gccaggatca gcaccgtcgc ggccagcgcg gagaggcgat ggctgtactc
     7741 gatgagcacg tcggcccgca gcggcgggat caccgagccg tggcagaggg gccagtccgg
     7801 gcacgcgagg cccgagccgg tcgcgctgac cacgttgccg agggtcatga gcacgaagac
     7861 cgcggccacc gcggcgaggg cgatcttagc cgacacgggt cgcggtccgg gtggggtgca
     7921 ccgcgccgtc gtcgcctccg gcggcctgcc agcggaagaa gacgacggag agcgcggcca
     7981 ggaagatcat gccgcccggg atccacatga tgagcccgcc caggtgctga tcggcctgcg
     8041 gggtcagccg cgcccagatg cggggagccg ccgcgtagta cgggtagagc aggctgtccg
     8101 ccatcgagat gaagatcgcc accatcacca tcggcagccc gaccacgaag aggtacagca
     8161 tctgcaccgg gtagggggcg cgcggcagct cctcgagcgg cgacagcatc ggccaccaca
     8221 ggatcaccga ggccaccatg atcatgaggt gctcgacgat gtgcaccggg tgctgctcca
     8281 tggccaggtt gtacagcggc ggcaggtgcc acgccaccag gaccacgttg aaggtggcga
     8341 acgcgccgac cggccgggtc agcgcccggc ccagctggag caggcgcgcg tcgcgcagga
     8401 gcggccgcgc catccagccg ggcgtgccgt agagcaggag cgggggcatc gcgaagacca
     8461 gcagcatgtg ctggatcatg tgcgcgctga acaggtagta gtccgagagg tcgtgcaccg
     8521 ggccggtgag ggcgccgagc aggaccgcga gcgcgcccgc gaacgagacc accttggcgg
     8581 gctccacgcg ggcctcgggg tccagccggt ggcgccagag cgtcgcggcc gcgtagacca
     8641 ctccgagggc gagcaggccg ccgacgacgc tgggatcggg ggaccaggcg gtccaatcga
     8701 agccgtcggg cccgaggggc ggcggccccg cgggcccggg tactacgtgc gccatgtcag
     8761 agaacgacgc ggggcagcag cacgaaggcg ccgaagagcg ccatgacggc caggatgatc
     8821 acgatggcga tgagcagcgg tcccacgaac agcgcgcgca tgatcgggtg gtcgtagcgg
     8881 aggtgcatga agaagcccac caccaggaag aacttcaccg ccgacatcgc gagcaggctg
     8941 atcacgagca ccgagcccgg gatgccgggg atgtagaggg aggccacctc caccgcggtg
     9001 atgatcgcga gcacgatggc cacgttgatg taggtcttga cggtggcgtg gccgcctcct
     9061 cctccgtgcg tcgcagcggc aacggcgtgc tgatcggcca tgtcgcggct cctaggggat
     9121 gaggtagatg acggtgaaga tggcgatcca cacgatgtcg acgaagtgcc agtagagacc
     9181 ggcgatctcg accttggtgt agtcgcgggc gtcgagcttg ccccgcatgc ccagcatcac
     9241 gaggatgagc agccagatca ccccgccggt gacgtgggcc ccgtggaagc cggtgagcac
     9301 gaagaagctc gacccgaaga ggctggtgga cagcgtgagg ttgtggtgca ggacgaagct
     9361 ggtgaactcg tagacctggt tcagcacgaa gtgcagcccc agcatcgcgg tcacgaagag
     9421 ccagcgggtc gcgggcttga tccggttctg ctgcagggcg gcgagcgcga gcaccatggt
     9481 cagcgacgag gcgagcagca gggccgcgct gtacgaggtg agcgggatgt cgaagacctc
     9541 gtggggcagc ggtccggaga tggacttgcc cttgtaggcc aggtacgtcc cgatcaggga
     9601 gccgaagaac atgcagtcgg agccgaggaa cgcccacatc cccaccttcc ggttgtcgag
     9661 accggtgttg ccgatgttgg gatcgtggac ggcgtggacg gcggcgtgat cggccacggg
     9721 ggctctccta gtgtccgtgc ccggcggggg gccggtgcag ctccatggcg aacttgtaca
     9781 tgaggtagag cgtcagcagc ccgccgatcg cgacctggta catgctgatc atcaggccgg
     9841 tgaccatcgc cgcgagcgcg agcccgagga acagcggcca gtaggacgtc ggcggcatgt
     9901 ggatcgccgc gatctcggcc gcggtcaccg gggccggctt gggccgcggg gcgctcccgt
     9961 gcccgtcgcc gtgcttggcg agccagaaat cgtcgcggtc ctgcacctgg ggcacccgcg
    10021 cgaagttgta gaccgggggc ggggagggga tcgcccactc gagcgtgcgc gcgtcccacg
    10081 ggtcgttgcc ggcgacctgg ccgcccttca tcgtccggat cacgttggcg atgaagaaga
    10141 gcagcgacac cgcgatcagg aaggccccga tcgtcgagac catgttccag aagttccagc
    10201 cgaggtcggg ggcgtaggtg tagatccgcc gcggcatccc gatgagcccg aggtagtgct
    10261 gggggaagaa ggtcatgttg aagcccagca tcatgagcca gaagtgccac aggccgaggc
    10321 gctcgtcgag cagcttgccg gtgatcttcg gccaccagta gtagatgccg gagaagagcc
    10381 cgaagatcga gcccccgaag agcacgtagt ggaagtgggc caccacgaag taggtgtcgg
    10441 tctgctgcag gtcgaccggc ggggaggcgt gcatgatccc ggagaggccg cccatcgtga
    10501 acatcgcgat gaagccgagc gagaagtaga gcggggtccg gaagcggatg gacccgcccc
    10561 agagggtgcc gatccagttg aggatcttca ccccggtcgg caccgcgatc agcatggtgg
    10621 agatcgagaa ggcggcgtcc gccaccggcc ccatgcccac cgagaacatg tggtggctcc
    10681 acaccccgaa gccgaagaag ccgatcatga tgccggagta gatcaccacc ggcgccccga
    10741 agagcggctt gcgcgagaag gtgggcagca cctccgagac gatgcccatc gcgggcagga
    10801 tcaggatgta gacctcgggg tggccgaaga tccagaagag gtgctgccag aggagcgggt
    10861 ccgcgcccgc ggcgggcgcg tagaagaggg tgccgaagaa ccggtcgaac atgaggagga
    10921 tgagcgccac cgtgatcgcg gggaaggcga gcacgagcag gaactgggtc acgaagctca
    10981 tccagatgaa caccggcatc cgcatgaggg tcatgccggg cgcgcgcatg ttgatgatcg
    11041 tcaccaggaa gttgaagccg gccagcatcg aggagaggcc gaggatctgc aggccgagca
    11101 tccagaagtc gatggtgtgc ttcggggaga actggatcga cgtcaggttg gcgtagccga
    11161 accagccccc gtccggggcg agcccgaaga gccagctcga gttcaggaac agggcgccgg
    11221 agaggaagat ccagtaggag agcgcgttca ggcgcgggaa tgcgacgtcg cgcgccccga
    11281 tcatgagcgg gatcatgtag ttgaagaagg ccgagctcag cggcatcacg gccaggaaga
    11341 tcatggtggt gccgtgcatc gtgaacagct cgttgaagcg ctcggggccg accagggtgt
    11401 tgtcgggccg cgcgagctgc agccgcatga tgagggcctc gatgccgccg atcaggaaga
    11461 agatgaaggc ggacgtgccg tagaggatcc cgatccgctt gtgatcgacg gtggtgagcc
    11521 aggaccagac gatgccccag aaggtctccg tctcgacctt cgagccgtgc atgaacgcgg
    11581 cctgagtggc catggggtcc ccttatttca ggctgagcag gtaggccgcg atggccttgc
    11641 tctgctcgcc ggtcattccg aggttcggca tgagggcccc gggcttcatg tgatcggggt
    11701 tctcgaccca cttggcgatg ttgtccggcg tcgactccat gagcgacccc gcgaaccgct
    11761 tgcggctggc gaagtgggtg aggtcgggac cgatgtgccc ggccgagacc ccggtgatgg
    11821 tgtggcaccc cacgcaggtg gactggccga agatcgtctt gccctgctgg gcgagcgcgt
    11881 cggcggactc caccggggga gcctgctggg ccttgaccca cttctcgaag tcggcgggct
    11941 cgtggacgat cacccggaag cgcatgttgg cgtgcgagat gccgcagtac tccgcgcact
    12001 ggcccgggta gtcgcccgcc acctcggggg tgagcgtgat gtggttgatg cggtgcggga
    12061 ccacgtcgcg cttgccgccg agctgcggca tccagaagga gtggatgacg tcgggcccgt
    12121 tgagcaggaa ggtcgcggtc tggccgaccg ggaggtggag ctcgttggcg gtggtgatct
    12181 tgaactgcgg gtacttgaac tcccaccacc actgcttgcc gaagacctcc acccgcagcg
    12241 cggtggccgc gggcgcctcc tgggtcttga agatcacccg gatggtcggg atcgcgaaca
    12301 cgaggaggat caccgcgaag gcgatggtcc agccgacctc gagggcggtg tggccgtgca
    12361 cctgcttcgg gatcggcgcg cccgcgcgat cgcggaagcg ccagcagacg taggccaggc
    12421 cgacctccac cacgatgaag atgccgagcg tccaccacga gatcagcatg aagatctcgt
    12481 gggtcatctt cccgaagtcg gacttcggca tgaccgtgct catcggggtg tcccagttcc
    12541 agcccaccgc gctgcaggag ctggcgagga gcaggaccgc gccgagcagg caggcctgcg
    12601 tccagtcgaa gcgcctccgg gggcggggga tcgtcgcggg gggcttcatc atggcgtggt
    12661 gtgggtcctc tagcgcgggg tcaccggcga ctgggccggc gcgccggtgg ccgcgaccgc
    12721 gggccggtga acgtcgacca cggccaccca gctccgggcg gccaggaaga gcacgaggag
    12781 caccgggatg caggtccaca ggagatcggt tccacgacgg gcgctgcgcg aggacaccgc
    12841 ccggcgcgat ctcagcacga aagccagaga cttggcctgg gcgaccagga acacggtcag
    12901 gaccagggcc agcagcaggt gcactatcgt gggcacaaca tcctcattga ggtaatggcg
    12961 ccggaatcac gcggaagtgt acttgatccc gcgcaccatt gccaagtgga aactttcaca
    13021 tgcagccgct gcgctcgcgt cccggcgcgg ctcacccggc caggcgcagg agcgtctgga
    13081 gcagcgcgtt ggtgccccgc ccgatcgcct tccagtcgct catctcgtcg gggcggtggc
    13141 tgcgcccgcc gcgggacggg atgaagagca tgcccgaggc ggtgaccccc gcgaggttct
    13201 gcgcgtcgtg gccggcgccc gacggcatcc gccgggtgcg aagccgcagc gcgtcgcagg
    13261 ccgcctgcac cgcgtccatc acccgcgggg cgcagcgggc cgggtcggtg cgcgagagcg
    13321 tgtcgacgac cacgcgcagg ccccggcgcc gcgccaccgt gcgggccagg gtcacgcact
    13381 cgcggtcgag ccgggcgagg atccgcgcgt cgagctcccg catctcctgc atcagcaccg
    13441 cccgggccgg gacgatgttg gagacgcccg gggccagctc gatgcgcccg aagttggtca
    13501 cgctccggcc gctgcccttc cggacgatgt gctcgcgcgc cttgagcgcg tactcgcagg
    13561 cggccaggaa ggcgtccttg cgtcgcgcca tcggcgtggt gccggcgtgg tccggctcgc
    13621 cgatgaaggt gaggcggttc cggcggatgc ccacgatgcc ttccacgagg ccgatgggga
    13681 tgcgcgcctg ctcgaggtgc gggccctgct cgatgtggag ctccacgtag gcgtgcaggg
    13741 tgcgcggatc gcagcgggcc tccgcgaccc ggctcgcgtc gtagcccgcg cgcgccatcg
    13801 cctccaccag cgattccccg tcgagcgagc gcagggtggt gatctgggcc aggtcgagcc
    13861 ggccggtgaa cgcccgcgag ccgaagaggc tgccgtagcg cccctcctcg tcgctccacg
    13921 ccaccaccgc gagcggccgc cgcacgggca gacccacctc cttgacagtc tggaggcact
    13981 cgaggccggc caggacgccg agggcgccgt ccagcgggcc gccctcgggc acggtgtcga
    14041 tgtgcgagcc ggtgaggacc gccgcgccct cgccgggcag catgccgaag gtgttgcccg
    14101 ccgcgtcgac cctcgtctgg aggcccgccg ccttcatccg ggacagcagc cacgcgcggg
    14161 cctgctcgtg aaccgggctc cagcaggagc gcgtgatgcc gtggccgtcg gcgttgcgac
    14221 cgtaggtgga gagggtctcg atgtggtcct tcaggcgcgc cagggtgatc gaggaggtgg
    14281 ccatggacgc ggcgagtata ccgcacactc cggacggccc gggccgggcg gcgaagacct
    14341 tgacactttc ttgcgcagac tcgtatcatg agcactgaac cttcattcag cctcatctcg
    14401 gtcaacctcg gtgagccgcc cccgcgagcg ggagggagta tggatttcac gctgacccct
    14461 gagcaggagt ccttccgcgg cgaggttcgc gcctggctgt cgaagcagat gcggcaggag
    14521 tgggtggcgc gtctctccaa gggctccgac attccgcgcc cggaggccta cgagctgctc
    14581 cggcgctggc aggggcagct ccacgaagcc ggcttcgtcg gcctcacctg gcccaaggaa
    14641 gcgggcgggc gggggctcac gttcatggag gagatgatcc tccagcagga gatggccctc
    14701 tccaaggctc cgccggtgct caacatcctg gccatcggca tggccggccc caccatcatc
    14761 gcctacggca ccgacgagca gaagcggcgg tacccgccca agatgctctc gtgcgaggag
    14821 atctggtgcc agggctactc cgagcccaac gcgggctccg acctggcgtc gctccagacc
    14881 cgtgcggtga aggacggcga ccactacgtc gtcaacggcc agaaggtgtg gacgtccctc
    14941 gcccacatcg cggactacat gatgctgctg gcccgcaccg atccgagcgc gcccaagcac
    15001 aagggcatca cctatttcct gctcgacatg aagagccccg gcg
//
LOCUS       DATMCV010000215         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_210857, whole genome shotgun sequence.
ACCESSION   DATMCV010000215 DATMCV010000000
VERSION     DATMCV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210857"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VNU03_05095"
     CDS             <1..241
                     /locus_tag="VNU03_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HWP73864.1"
                     /translation="RVFVDCLESAFDGVGDILAPIASGAIRREDVRGDLYDLIGGGVP
                     GRTGPDDITFFKNAGGGHLDLMTAQAIMAALDDGP"
     gene            258..1802
                     /locus_tag="VNU03_05100"
     CDS             258..1802
                     /locus_tag="VNU03_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HWP73865.1"
                     /translation="MNGAESLARTLVAGGVNVCFTNPGTSEMHFVAALDKVEGIRCVL
                     GLFEGVVTGAADGYWRMADRPAATLLHLGPGLGNGLANLHNARKAASGIVNIVGEHAT
                     YHVKHDAPLTSDIEGIARPVSAWVRTSPSARAVAADGADAIVAARTAPGQVATLILPA
                     DTAWNDADGVAPVPAAPARAAVAAEAIERCAKALRSGEPAMLMLTGLAVRERGLTLAG
                     RIATATGARIIAQGSNARIQRGAGRVPIERLPYPVDQALAVLKDVRHLILVGAKAPVA
                     FFAYPDKPSVLTPDGCQIHPLASIAEDSLGALEALADAVGAKKNGEVVQQPGRPALPT
                     GALTPDTLAAALGALLPEGAIVSDESVTTGRGFFKATAGAPPHDWMNNMGGSIGLGLP
                     LATGAAVACPDRKVVCLEGDGSGMYTLQALWTQAREGLDVTTVVFANRTYAILRHELT
                     GVRAGTPGPKAADMLDIGRPDLDWVALARGMGVPASRATTADEFNKQFAAGLAAPGPY
                     LVEAVL"
     gene            1799..2182
                     /locus_tag="VNU03_05105"
     CDS             1799..2182
                     /locus_tag="VNU03_05105"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011849779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP73866.1"
                     /translation="MKKEIVRVGVERPNPNLSPATKFGNLVFVAGQTGRHPATGEVGK
                     DIREQTRNTLERIKVILAAAGTSLDNVLSAMTHLTRVQDLAAYNEEYAKYFPTDKPAR
                     TTVTVATLNSPELLIEITVIACIPD"
     gene            complement(2187..>2661)
                     /locus_tag="VNU03_05110"
     CDS             complement(2187..>2661)
                     /locus_tag="VNU03_05110"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73867.1"
                     /translation="EGAHGFGLLMSSLGAGAVMAGIGLTLLRRGRPSLRFLAVSATVL
                     SAGTAGLGVVDHFGAAALLLVALGACQILFSTGCNTTLQLTTPDALRGRVMGLYALAN
                     AGMTPFGSLLVGAIAEHLGVRAACMLGGGGGLVAVAVLVLVGRRAGLSWTLARES"
BASE COUNT          391 a         1036 c          928 g          306 t
ORIGIN      
        1 ccgcgtcttc gtcgactgcc tcgagtccgc cttcgacggg gtcggcgaca tcctggcccc
       61 gatcgcgagc ggggcgatcc ggagggagga cgtgcgcggc gacctctacg acctgatcgg
      121 cggcggggtg ccgggacgga ccggccccga cgacatcacc ttcttcaaga acgcgggcgg
      181 cgggcatctc gatctgatga ccgcgcaggc catcatggcc gcgctcgacg acggcccctg
      241 agcccgagga gacctcgatg aacggtgcgg agagcctggc gcggacgctg gtcgcgggcg
      301 gagtcaacgt gtgcttcacc aaccccggca cctccgagat gcacttcgtg gccgcgctcg
      361 acaaggtgga gggcatccgc tgcgtcctcg gcctcttcga gggcgtggtg accggcgcgg
      421 ccgacggcta ctggcgcatg gccgaccggc cggccgcgac gctgctccac ctgggccccg
      481 gtctcggcaa cgggctcgcc aatctccaca acgcgcgcaa ggccgcctcg ggcatcgtca
      541 acatcgtggg cgagcacgcg acgtatcacg tcaagcacga cgcgccgctc acctcggaca
      601 tcgagggcat cgcgcgcccg gtgtcggcgt gggtgcgcac ctcgccgagc gcgcgcgcgg
      661 tcgccgccga cggcgccgac gccatcgtgg ccgcgcgcac cgcgcccggc caggtggcga
      721 ccctcatcct ccccgccgac accgcgtgga acgacgcgga cggcgtcgcg ccggtgcccg
      781 ccgcgcccgc ccgcgccgcg gtcgccgcgg aggcgatcga gcgctgcgcg aaggccctgc
      841 ggtcgggcga gccggccatg ctcatgctga ccggcctcgc ggtgcgcgag cgcggcctca
      901 cgctggccgg gcgcatcgcg accgcgaccg gcgcgcgcat catcgcccag ggctccaacg
      961 cccggatcca gcgcggggcc ggccgcgtgc ccatcgagcg gctgccctac ccggtggacc
     1021 aggcgctcgc ggtcctgaag gacgtccgcc acctgatcct ggtgggcgcc aaggcgccgg
     1081 tggccttctt cgcctacccg gacaagccga gcgtgctcac gcccgacggc tgccagatcc
     1141 acccgctcgc ctcgatcgcc gaggactcgc tcggcgcgct ggaggcgctg gccgacgcgg
     1201 tcggcgcgaa gaagaacggc gaggtggtcc agcagcccgg ccgccccgcg ctgccgaccg
     1261 gcgcgctcac ccccgacacc ctggccgccg cgctcggcgc gctgctaccc gagggcgcga
     1321 tcgtctccga cgagtcggtg accaccggcc gcggcttctt caaggccacc gcgggcgccc
     1381 cgccgcacga ctggatgaac aacatgggcg ggtcgatcgg cctcggcctg ccgctcgcca
     1441 ccggcgccgc cgtcgcctgc cccgaccgca aggtcgtctg cctcgagggc gacggcagcg
     1501 gcatgtacac gctccaggcc ctgtggacgc aggcgcgcga gggcctcgac gtgaccacgg
     1561 tggtcttcgc caaccgcacc tacgcgatcc tgcgccacga gctcaccggc gtccgcgccg
     1621 ggacgcccgg ccccaaggcg gccgacatgc tcgacatcgg ccgcccggac ctcgactggg
     1681 tggcgctcgc gcgcggcatg ggggtgcccg cgagccgcgc gaccaccgcg gacgagttca
     1741 acaagcagtt cgcggccggg ctcgcggcgc ccggccccta cctcgtggag gcggtgctat
     1801 gaagaaggag atcgtccgcg tcggcgtcga gcgacccaat ccgaacctgt cgccagccac
     1861 caagttcggc aacctggtgt tcgtggccgg ccagaccggc cggcacccgg ccaccgggga
     1921 agtgggcaag gacatccgcg agcagacccg caacacgctc gagcgcatca aggtgatcct
     1981 ggccgccgcg ggcacctcgc tcgacaacgt gctgagcgcg atgacgcact tgacccgcgt
     2041 ccaggacctg gccgcctaca acgaggagta cgccaagtat ttcccgaccg acaagccggc
     2101 gcgcaccacc gtgacggtgg ccacgctcaa ctcgcccgag ctgctgatcg agatcacggt
     2161 catcgcctgc atccccgact gattctctag ctctcgcggg cgagcgtcca gctgagcccc
     2221 gcgcggcggc cgacgagcac cagcaccgcc accgccacca gcccgccgcc gcccccgagc
     2281 atgcaggcgg cgcgcacgcc gagatgctcc gcgatcgcgc cgaccagcag cgagccgaac
     2341 ggggtcatcc cggcgttggc cagcgcgtag agccccatca cccgcccgcg gagcgcgtcc
     2401 ggggtggtga gctggagcgt ggtgttgcag ccggtcgaga agaggatctg acaggcgccg
     2461 agcgcgacca gcagcagcgc ggcggcgccg aagtgatcca cgaccccgag cccggcggtc
     2521 cccgcgctca gcacggtcgc cgagacggcg aggaaccgga gcgagggccg gcccctccgg
     2581 agcagggtca gcccgatccc ggccatcacc gctcccgcgc cgagcgacga catgagcagg
     2641 ccgaacccgt gcgcgccctc g
//
LOCUS       DATMCV010000216         3810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_877781, whole genome shotgun sequence.
ACCESSION   DATMCV010000216 DATMCV010000000
VERSION     DATMCV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877781"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VNU03_05115"
     CDS             complement(<1..944)
                     /locus_tag="VNU03_05115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP73868.1"
                     /translation="MTFVLTPEQKALKAAVYDLCKQYPGEYWRDLDSRREYPEAFINE
                     LTKAGYLAVLIPEEYGGGGLGIMEAALILEEINRSGGNAGACHAQMYIMGTVLRHGSA
                     EQKQQYLPKLATGELRLQAFGVTEPNAGSDTTKLQTTAVRKGDRYVVNGQKMFISRVL
                     QSDLMLLLARTTPVDQVKKKTDGLSVFLIDIRELKGKGLDVRPLKMMMNHSTNALFFD
                     NMEIPAGSLIGQEGKGFSYILDGMNAERILVASDSIGDARWFIEKAVAYAGQRVIFGK
                     PIGSNQGVQFPIAKAHVNIEAADLMRTKAARMFDAGIP"
     gene            complement(1085..1879)
                     /locus_tag="VNU03_05120"
     CDS             complement(1085..1879)
                     /locus_tag="VNU03_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP73869.1"
                     /translation="MSSSYRTLNVAASAEGHVVTVELNRPEALNAMNTAMGEDLLRCF
                     EALRWDKTVRAVVFTGAGSKAFCVGGDLKEREGMTDETWRAQHVIFEAAAAGVLHCPV
                     PVIAAVEGFAMGGGCELAVLSDFVVCGETAVFAVPEVTRGIFPGVGGTQLLPRILGAP
                     MAKELIFTGRRVDAREARAIGLVNHVVPAGQARAKALEIAATIADNGPIAVRQAKKAI
                     AWGAETDLETGMALAIEAYNNTVTTEDRLEGVRAFNEKRKPRFQGK"
     gene            complement(1863..3056)
                     /locus_tag="VNU03_05125"
     CDS             complement(1863..3056)
                     /locus_tag="VNU03_05125"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP73870.1"
                     /translation="MNAALAGLLVADLTQNVAGPYCTQILGDMGAEVVKVERIGRGDD
                     ARAWGPPFWGRESATFMSVNRNKRSLAVDLKSADGRAVLERLLSRADVFVQSLRAGAA
                     EELDLGWPRLSALNPRLVYCSVTAFGTEGPLKDRPGYDPLMQAYGGIMSINGHPGQPP
                     ARVPVSVVDMGTGMWGVMGILAALRERDRTGRGALVTTALFDTALAWSVFQMSHYFAT
                     GEVPLPQGSGTAMIVPYEAFPVSDGWVMIAAASDALFVKTTGALGVPELARDGRFVDN
                     PRRVEHRAALQEALARVTRGLTAADVLERLQRAGVPSARVSTMDQVAGEPQTEASGML
                     PAMKHPRVPDFRAVALPIKWDGERPAPTRVPPLLGEHSTEVLAELGYDAATIRDLAER
                     HVVQL"
     gene            complement(3119..>3810)
                     /locus_tag="VNU03_05130"
     CDS             complement(3119..>3810)
                     /locus_tag="VNU03_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HWP73871.1"
                     /translation="TAGRRMTELLGLDPPPVAVTFAAAAPAGIPRVSRSGPAGCAYWK
                     QAGEGAVFYTEAADHFNCPVGSYTHGVALPPERAKELEDVVGTMVGLQYIRMEEVGAL
                     PRRKAAFGVAVYAPLALTPTPPDVVLVRGRARQIMLVAEAARAAGLAGDAAAMGRPAC
                     AMIPAVLEGPGGVTSLGCIGNRVYTGLGDDELYFTIPGAKVGEVVERLETVLTANREL
                     EKYHQARRASL"
BASE COUNT          517 a         1349 c         1341 g          603 t
ORIGIN      
        1 cacgggatgc ccgcgtcgaa catccgggcc gccttggtgc gcatgaggtc ggcggcctcg
       61 atgttgacgt gggccttggc gatcgggaac tgcacgccct ggttggaacc gattggcttg
      121 ccgaagatca cccgctggcc cgcgtaggcg accgccttct cgatgaacca gcgggcgtcg
      181 ccgatcgagt cggaggcgac caggatgcgc tccgcgttca tgccgtcgag gatgtaggag
      241 aatcccttgc cctcctggcc gatgaggctt ccggccggga tctccatgtt gtcgaagaag
      301 agcgcgttgg tcgagtggtt catcatcatc ttgagtgggc gcacgtcgag gcccttgccc
      361 ttcagctcgc ggatgtcgat gaggaacacg ctgagcccgt cggtcttctt cttcacttgg
      421 tccaccggag tcgtccgcgc gagcagcagc atcagatccg actggagcac gcgcgagatg
      481 aacatcttct ggccgttgac cacgtagcgg tcgcccttgc ggaccgcggt ggtctgcagc
      541 ttggtggtgt ccgagcccgc gttgggctcg gtgaccccga acgcctgcag ccgcagctcg
      601 ccggtggcga gcttgggcag gtactgctgc ttctgctccg cggagccgtg ccggagcacg
      661 gtgcccatga tgtacatctg ggcgtggcag gcccccgcgt tgccgcccga gcggttgatc
      721 tcctcgagga tgagggcggc ttccatgatg ccgaggccgc ccccgccgta ctcctcgggg
      781 atgaggaccg cgaggtagcc cgccttggtc agctcgttga tgaaggcctc cggatactcg
      841 cgccgggagt cgaggtcccg ccagtattcg ccggggtact gcttgcagag atcgtagacc
      901 gcggccttga gggctttttg ctctggggtg agtacgaagg tcatgtcggc agtcatgcgt
      961 gacccctctt catcttcaac atggctgaag ccatgttgac cgtgggcgag atggtcgggg
     1021 cgacccgttg cttgtcgtcc gcctcgctgc ttgccgtcca tctcgcattg cagtttctcg
     1081 ccatttattt gccctggaat ctgggcttgc gcttctcgtt gaaggcgcgc acgccctcga
     1141 ggcggtcctc ggtggtcacg gtgttgttgt aggcctcgat ggccagcgcc atgccggtct
     1201 cgagatcggt ctcggcgccc caggcgatcg ccttcttggc ctggcgcacc gcgatgggtc
     1261 cattgtccgc gatggtggcc gcgatctcca gcgccttcgc ccgcgcctgg cccgccggca
     1321 ccacgtggtt gacgagcccg atcgccctgg cctcgcgcgc gtccacccgg cggccggtga
     1381 agatcagctc cttcgccatc ggcgcgccca ggatgcgggg caggagctgg gtgccgccca
     1441 cgcccgggaa gatgccgcgg gtgacctcgg gcaccgcgaa gaccgcggtc tcgccgcaca
     1501 ccacgaagtc ggagagcacc gccagctcgc agccaccgcc catcgcgaag ccctccaccg
     1561 ccgcgatgac cggcaccggg cagtggagca cgcccgcggc ggccgcctcg aagatgacgt
     1621 gctgggcgcg ccacgtctcg tcggtcatgc cctcccgctc cttgaggtcg cctcccacgc
     1681 agaacgcctt gcttccggcc ccggtgaaca ccacggcgcg caccgtttta tcccagcgta
     1741 gtgcttcgaa gcagcggagc aggtcctcgc ccatcgcggt gttcatcgcg ttgagggcct
     1801 cggggcggtt cagctccacc gtgaccacgt gcccctcggc ggaggcggcg acgttcagcg
     1861 tcctatagct ggacgacatg gcgctccgcg aggtcccgga tggtggcggc gtcgtagccc
     1921 agctcggcga gcacctccgt cgaatgctcc ccgagcagcg gcggcacgcg ggtgggggcg
     1981 ggccgctcgc cgtcccactt gatcggcagc gccaccgcgc ggaagtcggg cacccgcggg
     2041 tgcttcatcg cggggagcat cccgctcgcc tcggtctgcg gctcgcccgc gacctggtcc
     2101 atggtcgaca cgcgcgccga cgggacgccg gcccgctgca gccgctccag cacgtcggcg
     2161 gcggtgagcc cgcgcgtgac ccgcgcgagc gcctcctgca gggccgcgcg gtgctcgacg
     2221 cggcgcgggt tgtccacgaa gcggccgtcg cgggccagct cgggcacgcc gagggcgccg
     2281 gtcgtcttga cgaagagcgc gtcggaggcg gccgcgatca tcacccagcc gtccgagacc
     2341 gggaacgcct cgtagggcac gatcatcgcg gtgcccgagc cctgcgggag cgggacctcg
     2401 ccggtcgcga agtagtggct catctggaag accgaccacg cgagcgcggt gtcgaacagc
     2461 gcggtggtca ccagcgcccc gcgtccggtg cggtcgcgct cgcgcagcgc ggccaggatg
     2521 cccatcaccc cccacatgcc ggtgcccatg tccaccaccg agaccggcac cctcgcgggc
     2581 ggctggccgg ggtggccgtt gatcgacatg atcccgccgt aggcctgcat cagcggatcg
     2641 tagccgggcc ggtccttgag cgggccctcg gtgccgaagg cggtgaccga gcagtacacc
     2701 aggcgcgggt tgagcgcgga gagacgcggc cagccgaggt cgagctcctc cgccgcgccc
     2761 gcccgcagcg actgcacgaa cacgtcggcg cgggagagga gccgctccag caccgcgcgg
     2821 ccgtcggcgc tcttcagatc caccgcgagg ctccgcttgt tgcggttcac gctcatgaag
     2881 gtggcgctct cgcggcccca gaagggcggt ccccaggcgc gcgcgtcgtc gccccgtccg
     2941 atgcgctcca ccttgacgac ctcggccccc atgtcaccca ggatctgggt gcagtagggc
     3001 ccggcgacgt tctgggtcag gtcggcgacg agaaggccgg cgagggccgc gttcatgggg
     3061 gatcgattat agccccatcc cccggacctt cgacggcgag cctggagaca ggcgacggct
     3121 agaggctggc ccggcgcgcc tggtggtact tctcgagctc gcgattggcg gtcagcaccg
     3181 tctcgaggcg ctcgacgacc tccccgacct tggcgcccgg gatcgtgaag tagagctcgt
     3241 cgtcgcccag gccggtgtag acccggttgc cgatgcagcc gaggctggtg acgccgcccg
     3301 gcccctcgag caccgcgggg atcatcgcgc aggcggggcg gcccatcgcg gccgcatcgc
     3361 ccgcgagccc ggccgcccgc gcggcttcgg ccaccagcat gatctggcgg gcccggcccc
     3421 gcaccagcac cacgtccggc ggcgtgggcg tcagggcgag cggggcgtac accgcgacgc
     3481 cgaacgcggc cttccggcgc ggcagcgcgc cgacctcctc catgcggatg tactgcaggc
     3541 ccaccatggt gccgaccacg tcttcgagct ccttcgcgcg ctcgggcggc agcgcgaccc
     3601 cgtgggtgta ggagccgacc gggcagttga agtgatcggc ggcctcggtg tagaagaccg
     3661 ccccctcccc ggcctgtttc cagtaggcac agccggcggg gccggacctg gacacccgcg
     3721 gaatgccggc gggcgcggcg gcggcgaagg tcactgccac cgggggcgga tccagcccga
     3781 gcagctcggt catgcgtcgt ccggcggtgc
//
LOCUS       DATMCV010000217         2377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_211036, whole genome shotgun sequence.
ACCESSION   DATMCV010000217 DATMCV010000000
VERSION     DATMCV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211036"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VNU03_05135"
     CDS             complement(<1..362)
                     /locus_tag="VNU03_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWP73872.1"
                     /translation="MLTGRRFTLPLQALEPMDLAGDGSATLLLLNPTGGVLGGDRLET
                     EVSLGPGSRVCLSTPSATRVYRSPEHPAVQRVTVTLGAGAVLEWVPDHLIPSPGARLR
                     QATEVTMAPDATLLYLDS"
     gene            complement(430..1056)
                     /gene="ureG"
                     /locus_tag="VNU03_05140"
     CDS             complement(430..1056)
                     /gene="ureG"
                     /locus_tag="VNU03_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HWP73873.1"
                     /translation="MSAPGAVPRPLKIGIGGPVGSGKTALVETLARRLRDHFDMAVIT
                     NDIYTQEDAQFLVRRGALPADRVVGVETGGCPHTAIREDASVNLEAVHDLVTRFPDLE
                     LLFVESGGDNLSATFSPELVDATIYVIDVAEGDKIPRKGGPGIVRSDLLVINKIDLAP
                     YVGADLGVMERDAKRMRGERPFVFTNLREGDGADAIVSWIRRDLLFAP"
     gene            complement(1053..1742)
                     /locus_tag="VNU03_05145"
     CDS             complement(1053..1742)
                     /locus_tag="VNU03_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HWP73874.1"
                     /translation="MTDPRLLSLLHFADSALPTGGYAHSFGLERYCQAGLVRDRHGLE
                     RFLLTQLEGATGPCDATAAAGALRALARGDVEACHRLDEELEAMKVVREFREGSRQMG
                     RQTLRVAATLTGDGRLATYLEAVEARRAPGHHAVAFGMTAGALGWEAGAAATALLYST
                     TALLVGAALRLLPIGQLEGQSVLWSLHPVITRVAREAAERAPGDLWSFTPGQDIQGML
                     HERMDARLFRS"
     gene            complement(1735..2169)
                     /locus_tag="VNU03_05150"
     CDS             complement(1735..2169)
                     /locus_tag="VNU03_05150"
                     /inference="COORDINATES: protein motif:HMM:NF017045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HWP73875.1"
                     /translation="MITDLPRAIEPAALAGKARDTLSLTWEERRWTRKRVTTTGGREV
                     ALALPTGSVLEPGAILAVEEDWYLAVEARPEAVLACFPSSHSEALRIAFDVGNRHFTL
                     AVEAGALVVPDDPAMSQLLSRLGVRWERRESLYAPLGGGGDD"
     gene            complement(2180..>2377)
                     /gene="ureC"
                     /locus_tag="VNU03_05155"
     CDS             complement(2180..>2377)
                     /gene="ureC"
                     /locus_tag="VNU03_05155"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346107.1"
                     /note="ureases catalyze the hydrolysis of urea into
                     ammonia and carbon dioxide; in Helicobacter pylori the
                     ammonia released plays a key role in bacterial survival by
                     neutralizing acids when colonizing the gastric mucosa; the
                     holoenzyme is composed of 3 ureC (alpha) and 3 ureAB
                     (gamma/beta) subunits; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWP73876.1"
                     /translation="TRLGLRKMTAAVRGCRGLAKSAMIHNAYCPRIEVDAETYEVRAD
                     GQLLTCEPARVLPMAQRYFLF"
BASE COUNT          328 a          882 c          867 g          300 t
ORIGIN      
        1 cagctgtcga ggtagagcag cgtggcgtcc ggcgccatcg tcacctcggt cgcctggcgc
       61 agccgggccc ccggcgacgg gatcaggtga tccggcaccc actcgaggac cgctcccgcg
      121 ccgagcgtga ccgtcacccg ctggaccgcg ggatgctcgg ggctccggta cacgcgtgtg
      181 gccgacgggg tcgagagaca gacgcggctg ccgggcccga gcgagacctc cgtctccagg
      241 cggtcgcctc ccagcacgcc gccggtcgga ttcaggagca ggagggtggc cgagccgtcg
      301 cccgcgaggt ccatcggctc gagggcctgg agcgggagcg tgaagcggcg gccggtgagc
      361 acggtgcggc cgtcgcgcgc ctcgaaggcg agacggagcc gcccgtcccg ccccgcgcgg
      421 ccgcgcggct cagggcgcga agaggaggtc gcgtcggatc caggagacga tggcgtcggc
      481 gccgtcgcct tcccggagat tggtgaagac aaagggccgc tctcctcgca tgcgcttggc
      541 gtcgcgctcc atgaccccga ggtcggcgcc cacgtagggc gcgagatcga tcttgttgat
      601 cacgagcagg tccgagcgca cgatccccgg cccgcctttg cgcgggatct tgtcgccctc
      661 cgccacgtcg atgacgtaga tggtcgcgtc gaccagctcc gggctgaagg tcgcggagag
      721 gttgtcgccg ccgctctcca cgaagagcag ctcgagatcc ggaaagcgcg tcaccagatc
      781 gtgcaccgcc tcgaggttca ccgaggcgtc ctcgcggatg gcggtgtgcg ggcagccccc
      841 ggtctccacc cccaccaccc ggtcggcggg cagcgccccg cgccggacca ggaactgggc
      901 gtcctcctgc gtgtagatgt cgttcgtgat caccgccata tcgaagtggt cgcgcaggcg
      961 ccgggccagc gtctccacca gcgcggtctt gcccgagccc accgggcctc cgatgccgat
     1021 cttgagcggc cggggcaccg cgccgggagc gctcacgagc ggaagagccg cgcgtccatg
     1081 cgctcgtgca gcatgccctg gatgtcctgg cccggggtga agctccagag atcgccgggg
     1141 gcccgctccg cggcctcgcg ggccacccgg gtaatcaccg gatgcaggct ccagagcacc
     1201 gactgcccct cgagctgccc gatgggcagg agccggagcg ccgcgccgac cagcagcgcg
     1261 gtggtcgagt agagcagcgc ggtcgcggcc gcgcccgcct cccagccgag ggcgcccgcg
     1321 gtcatgccga acgcgaccgc gtgatggccg ggggcccggc gcgcctcgac cgcctcgagg
     1381 taggtcgcca ggcggccgtc cccggtgagg gtcgcggcga cgcgcagggt ctgccgcccc
     1441 atctgccggc tgccctcgcg gaactcccgc acgaccttca tggcctccag ctcctcgtcc
     1501 aggcgatgac acgcctcgac gtcgccccgg gcgagcgcgc gcagcgcacc cgcggccgcg
     1561 gtggcgtcgc aggggccggt ggcgccctcg agctgcgtga gcaggaagcg ctcgaggccg
     1621 tggcggtcgc gcaccagccc cgcctggcag tagcgctcga gcccgaacga gtgcgcgtag
     1681 ccgccggtgg gcagcgcgct gtccgcgaag tgcagcagcg acagcagtcg cggatcagtc
     1741 atcgccgcct ccgccgagcg gcgcgtacaa gctctcacgg cgctcccacc gcacccccag
     1801 gcgcgagagg agctgggaca tcgcggggtc gtccgggacc accaaggccc cggcctcgac
     1861 cgcgagggtg aagtggcggt tgcccacgtc gaacgcgatc cgcagcgcct ccgagtggct
     1921 cgacgggaaa caggccagca ccgcctccgg ccgggcctcc accgcgaggt accagtcctc
     1981 ctccaccgcg aggatcgccc ccggctccag cacgctcccg gtgggcaggg cgagcgcgac
     2041 ctcgcggcca ccggtggtcg tgacgcgctt gcgcgtccag cgccgctctt cccacgtgag
     2101 gctcaaggtg tcgcgcgcct tgcccgccag cgcggccggc tcgatcgccc gcgggaggtc
     2161 cgtgatcacg gtcatcgcgc tagaacagga agtagcgctg ggccatcggc aacacgcggg
     2221 cgggctcgca ggtgaggagc tggccgtcgg cccgcacctc gtacgtctcc gcgtccacct
     2281 cgatgcgcgg gcagtaggcg ttgtggatca tcgccgactt ggccaggccc cggcagccgc
     2341 gaacggccgc ggtcatcttg cgcaggccga ggcgggt
//
LOCUS       DATMCV010000218         5658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_544495, whole genome shotgun sequence.
ACCESSION   DATMCV010000218 DATMCV010000000
VERSION     DATMCV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544495"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VNU03_05160"
     CDS             <1..195
                     /locus_tag="VNU03_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73877.1"
                     /translation="SGDTLMLVRHDVVPGLMDEMQSMAIFADSPERLAAAGLARGDRV
                     RLTVRQEKDRLVAIEILKIR"
     gene            226..2307
                     /locus_tag="VNU03_05165"
     CDS             226..2307
                     /locus_tag="VNU03_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP73878.1"
                     /translation="MPKTPRRSRTMSLREAASVVFALVALLPILLLVYLLSRHELMHS
                     TEAQVGLLAAIGVSVLGFVVFRRMMDQISALAQGFIAPTEAKPETLRRVERASAVPGL
                     GEVTEIGQVRDAFYHMLDDLRGSTQRLEDLVFKLGTLNEMVELAARIPRIQDLLGLVL
                     QSTMRAVRATVGSIMILDRERTVLKLAASRGIPDEVIPAIEVKVGEGVAGKVVEMGEP
                     VLVDDIAADPRFNRTNNPQYGNGSFICMPIRVGDRVIGVINMAKKRDGGVSDALRLPP
                     FGSTDLQFLNALMTYIGYAVDNSRLLEEAQSSASQLQGVVDDLKSTQAQLVRGETLRA
                     MGQLSSGMAHHLNNLFAVILGRVELLMGKVQEASVRRSLEIIQRTAQDGAEVVRRVQR
                     FSRVQPVSDAVAVDLNQLVQEVVELTRPRWQDEAQLRGSRIEIAVEAGVIGAAAGEPA
                     PLREVLMNLLLNAADSITQAGKITLKTWTRDDRVYCSVADTGGGMPEEVRRRALEPFF
                     TTKGPKATGLGLSVAYGTVQRYGGTLTVESTEGQGTTVEVSLPTASAAASIARTPTGP
                     RSAPVVPLRILVIDDELQVRATLAEMLEEQGHSVTQAPGGREGLSYLDANPQLVDVVV
                     SDLGMPDMNGWDVAKAIQARWPQLPVGLITGWGETEITREERSRVNFVITKPFDKAVL
                     RETMSDIRPRG"
     gene            complement(2320..2697)
                     /locus_tag="VNU03_05170"
     CDS             complement(2320..2697)
                     /locus_tag="VNU03_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWP73879.1"
                     /translation="MKAKWMVVVFGPALLMLIGSLLAMLFLPHPVPKKATGGQRLYLT
                     YCASCHGANGHGAWRAWLFLLRPGDLGDARLIDTRSDEYLLTLIRNGGATIGRPGMPA
                     FGYHLKDEEIREVVRYLRTLPKR"
     gene            2757..4274
                     /locus_tag="VNU03_05175"
     CDS             2757..4274
                     /locus_tag="VNU03_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWP73880.1"
                     /translation="MRLVLMRHGEAGEADPTRWPGDRERPLTDAGRREHARVAGALRR
                     MGVTFDRILTSPLARARETADITARAYGGVPVPEPSELLGDRAEPARTLAGLAAVDAE
                     RLLCVGHEPTLSRLAALLISRDGSARVEMRKSGIAVIDCAGPAVSGRGTLRLHLRPDE
                     ITRLADGLPAPDASPSPDLFMSTVSAYQRSAALRGALDLDLFTAIGAGRETAATVAER
                     CRASVRGTRVLCDYLTVAGLLAKDGERYALTADSAAFLDRRSPSYVASAADFIYAPEL
                     RAAFADVAAAVRRGGTTLPEAGTVTAQHPVWVGFARAMAPLMRGAAHAVVETVEVDEA
                     RPLRVLDVAAGHGMFGIAFAQRYPRAEVAGLDWASVLAVARENARAAGVEARYRTIEG
                     SAFDADLGGPYDLILIPNFLHHFDPPTCERFLARARAALAPAGRAVTVEFVPDEGRVT
                     PPPAAMFALVMLCTTPAGDAYTLGELDSMFRRAGFASSELHVPGPGGPQVIISQR"
     gene            4285..5340
                     /locus_tag="VNU03_05180"
     CDS             4285..5340
                     /locus_tag="VNU03_05180"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HWP73881.1"
                     /translation="MRHRIAVIPGDGIGKEVLPEGIRVLEAAGRRFGVEFEWTHFDWS
                     CEVYARTGRMMPEDGLAQLRPFEAIYLGAVGFPGVADHVSLWGLLIPIRREFHQYVNL
                     RPVRLLRGVRSPLAGRGPADIDMLIVRENNEGEYSEIGGRLYRDTPHELAVQQAVFTR
                     RGCDRVIRYAFELAKKRPRHHVTSATKSNGIIHSMPYWDERFAAMAKHYPDVKTSQYH
                     IDILTAHFVQHPDWFDVVVASNLFGDILSDLGPAIAGSIGIAPGGNINPEKEYPSMFE
                     PVHGSAPDIAGKGIANPIAQIWTGAMMLEHLGHPEAAHAVVDAIERYVEAGKGLTRDL
                     GGTATTAEVGKALAAMF"
     gene            complement(5502..>5658)
                     /locus_tag="VNU03_05185"
     CDS             complement(5502..>5658)
                     /locus_tag="VNU03_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWP73882.1"
                     /translation="AMMLDHLGHPEAAHAVVDAIERYVEAGKGLTRDLGGTATTTEVG
                     KAIASMF"
BASE COUNT          806 a         2047 c         2001 g          804 t
ORIGIN      
        1 agcggcgaca ccctcatgct ggtgagacac gacgtggttc cgggcctcat ggacgagatg
       61 cagtcgatgg ccatcttcgc ggactcgccg gagcggctcg ccgcggcggg gctcgcgcgc
      121 ggagatcgcg tgcggctcac ggttcgccag gagaaggatc ggctggtggc gatcgagatc
      181 ctaaagattc ggtaaatctc tcacccttgc taaggtaggg acatcatgcc gaagaccccg
      241 cgccgatccc ggaccatgag cctccgcgag gcggcttcgg tggtcttcgc cctggtcgcg
      301 ctcctcccga tcctcctgct ggtctacctg ctctcgcgcc acgagctgat gcacagcacc
      361 gaggcgcagg tgggtctgct cgccgcgatc ggggtgtcgg tcctcggctt cgtggtcttc
      421 cggcggatga tggatcagat ctcagctctc gcccagggct tcatcgcgcc caccgaggcc
      481 aagcccgaga ccctccgacg ggtggagcgg gcctccgcgg tccccgggct cggcgaggtc
      541 accgagatcg ggcaggtccg cgacgcgttc taccacatgc tcgacgacct ccgcggctcg
      601 acccagcgcc tggaggatct cgtcttcaag ctcggcaccc tcaacgagat ggtcgagctg
      661 gccgcccgca tcccgcgcat ccaggacctg ctcggcctcg tgctccagtc gacgatgcgc
      721 gcggtgcgcg ccaccgtcgg ctcgatcatg atcctcgacc gggagcgcac ggtgctgaag
      781 ctcgccgcct cccgcggcat cccggacgag gtgatcccgg cgatcgaggt caaggtgggc
      841 gagggcgtgg ccggcaaggt ggtggagatg ggcgagccgg tcctggtcga cgacatcgcg
      901 gccgatcctc gcttcaaccg gaccaacaac ccccagtacg gcaacggctc gttcatctgc
      961 atgccgatcc gggtgggcga ccgggtcatc ggtgtcatca acatggccaa gaaacgcgac
     1021 gggggagtct ccgacgcgct gcggctgccc cccttcggct ccaccgatct ccagttcctc
     1081 aacgccctca tgacctacat cggttacgcg gtcgacaact cgcgcctcct cgaggaggcc
     1141 cagtcgtccg cttcccagct ccagggcgtg gtggacgacc tcaagtcgac ccaggcccag
     1201 ctcgtgcgcg gcgaaacgct gcgggcgatg ggccagctct cctccggcat ggcccaccat
     1261 ctcaacaacc tcttcgcggt gatcctgggc cgggtcgagc tgctcatggg caaggtgcag
     1321 gaggcgagcg tgcggcgctc cctcgagatc atccagcgca ccgcccagga tggcgccgag
     1381 gtggtgcggc gggtgcagcg cttcagccgg gtgcagccgg tctcggacgc ggtggcggtg
     1441 gacctcaacc agctggtgca ggaagtggtc gagctcacgc ggccgcgctg gcaggacgag
     1501 gcccagctac gcggcagccg catcgagatc gcggtcgagg ccggcgtcat cggcgcggcg
     1561 gcgggcgagc ccgccccgct gcgcgaggtg ctgatgaacc tgctcctcaa cgcggcggac
     1621 tcgatcaccc aggccgggaa gatcacgctc aagacgtgga cccgcgacga ccgggtgtac
     1681 tgcagcgtcg ccgacaccgg cggtggcatg cccgaggagg tccggcgccg ggccctggag
     1741 ccgttcttca ccaccaaggg ccccaaggcc accgggctcg ggctcagcgt ggcctacggg
     1801 accgtgcagc gctacggcgg caccctgacc gtcgagagca cggaggggca gggcacgacc
     1861 gtggaggtca gtctgcccac cgcttccgcg gcggcgtcga tcgcccggac tcccaccggt
     1921 ccccggagcg cgccggtcgt gccgctgcgc atcctggtca tcgacgacga gctccaggtg
     1981 cgcgccaccc tggccgagat gctcgaggag caggggcact cggtgaccca ggcgcccggc
     2041 ggccgcgagg gcctctccta cctcgacgcc aacccgcagc tcgtcgacgt ggtggtgagc
     2101 gacctcggca tgcccgacat gaacggctgg gacgtggcca aggccatcca ggcccgctgg
     2161 ccgcagctgc cggtcggtct catcaccggc tggggcgaga ccgagatcac ccgcgaggag
     2221 cgcagccggg tgaacttcgt gatcaccaag cccttcgaca aggccgtcct gcgcgagacg
     2281 atgtcggata ttcgcccgcg cggctaggcg cccgcgcggc tagcgcttcg gcagcgttcg
     2341 caggtaccgc accacctccc ggatctcctc gtccttcaag tggtagccga aggcgggcat
     2401 gccgggcctg ccgatcgtcg cccccccgtt ccggatcagc gtgaggaggt attcgtccga
     2461 gcgcgtgtcg atgagccgcg cgtcgcccag gtcgcccgga cgcaggagga agagccacgc
     2521 gcgccacgcg ccgtggccgt tggcgccgtg gcaggaggcg cagtaggtca ggtacaggcg
     2581 ctggccgccg gtggccttct tggggaccgg atgcggcagg aagagcatcg ccaggaggct
     2641 cccgatcagc atgagcagag cgggaccgaa caccacgacc atccacttcg ctttcacggc
     2701 cgtcgagcat accacgcggg gcggcgcgcg ctcggactcg tgcgatagta gtcgccatgc
     2761 gtctcgtcct catgcgccac ggcgaggccg gagaggcgga tccgacccgc tggcccggcg
     2821 accgcgagcg tccgctcacc gacgccgggc gccgcgagca tgcgcgggtg gccggcgcgc
     2881 tccgccgcat gggcgtgacc ttcgaccgca tcctcacgag cccgctcgcc cgcgcgcgcg
     2941 agaccgccga catcaccgcc cgcgcgtacg ggggcgtgcc ggtgccggag ccgagcgagc
     3001 tcctcggcga ccgggccgag ccggcccgca ccctggccgg gctcgcggcg gtcgatgcgg
     3061 agcggctcct gtgcgtgggg cacgagccca cgctctcccg cctggccgcg ctgctcatca
     3121 gccgcgatgg gtcggcccgg gtcgagatgc gcaagagcgg gatcgcggtc atcgactgcg
     3181 ccggcccggc ggtctccggg cgcggcacgc tgcgcctgca cctgcgaccc gacgagatca
     3241 cccgcctggc cgacggcctc ccggccccgg acgcgtcacc ctctcccgac ctgttcatgt
     3301 ccaccgtgag cgcgtaccag cggagcgccg cgctccgcgg cgcgctggac ctggacctct
     3361 tcaccgcgat cggcgcgggg cgcgagaccg cggccaccgt cgccgagcgc tgccgcgcct
     3421 ccgtgcgcgg cacccgcgtc ctctgcgact acctcacggt ggccggcctg ctcgccaagg
     3481 acggcgagcg ctacgcgctg accgcggact cggccgcctt cctcgaccgg cgctcgccgt
     3541 cgtacgtggc ctccgcggcg gacttcatct acgcgcccga gctgcgcgcg gccttcgccg
     3601 acgtggcggc ggcggtgcgt cggggcggca ccacgctgcc cgaggcgggc accgtgacgg
     3661 cgcagcatcc ggtgtgggtc gggttcgccc gggccatggc gccgctgatg cggggcgccg
     3721 cgcacgcggt ggtggagacg gtcgaggtgg acgaggcgcg gccgctccgg gtgctcgacg
     3781 tggcggccgg gcacgggatg ttcggcatcg cgttcgcgca gcgctacccg cgcgcggagg
     3841 tggccgggct cgactgggcg agtgtgctcg cggtggcgcg cgagaacgcg cgcgcggccg
     3901 gagtcgaggc ccgctatcgg accatcgagg gcagcgcctt cgacgcggac ctcggcgggc
     3961 cctacgacct gatcctcatc cccaacttcc tccaccactt cgaccccccg acctgcgagc
     4021 ggttcctcgc gcgggcccgg gccgccctgg cgcccgccgg ccgcgcggtg acggtggagt
     4081 tcgttcccga cgaggggcgg gtgaccccgc ccccggccgc gatgttcgcc ctcgtgatgc
     4141 tctgcacgac gccggcgggc gacgcctaca ccctcggcga gctcgattcc atgttccgcc
     4201 gggccgggtt cgcctcgagc gagctgcacg tcccggggcc cggcggcccc caggtcatca
     4261 tctctcagcg ttgaggaggc tttcatgagg catcggatcg cggtgattcc cggcgacggc
     4321 atcggcaagg aggtcctgcc cgaaggcatc cgggtcctgg aggcggccgg gcgtcgcttc
     4381 ggggtggagt tcgagtggac ccacttcgac tggagctgcg aggtctacgc ccgcaccgga
     4441 cgcatgatgc ccgaggacgg cctcgcgcag ctacggccct tcgaggcgat ctacctcggc
     4501 gcggtgggct tccccggggt ggccgaccac gtctcgctct gggggctgct catcccgatc
     4561 cgccgcgagt tccaccagta cgtgaacctg cggccggtgc gcctgctgcg cggggtgcgc
     4621 tcgcccctgg ccggacgcgg gcccgcggac atcgacatgc tgatcgtgcg ggagaacaac
     4681 gagggcgagt actccgagat cggcgggcgg ctctaccggg acacgcccca cgagctggcc
     4741 gtgcagcagg cggtgttcac ccggcgcggc tgcgaccggg tgatccgcta cgcgttcgag
     4801 ctggccaaga agcggccccg gcaccacgtg acctcggcca ccaagtcgaa cgggatcatc
     4861 cacagcatgc cctactggga cgagcgcttc gcggcgatgg cgaagcacta ccccgacgtc
     4921 aagaccagcc agtaccacat cgacatcctc accgcccact tcgtgcagca ccccgactgg
     4981 ttcgacgtgg tggtggcctc gaacctcttc ggggacatcc tctccgacct gggacccgcc
     5041 atcgcggggt cgatcggcat cgccccgggc ggcaacatca accccgagaa ggagtacccg
     5101 tcgatgtttg agccggtgca cggctcggcg cccgacatcg cgggcaaggg gatcgcgaat
     5161 ccgatcgccc agatctggac cggcgcgatg atgctcgagc acctcggcca cccggaggcg
     5221 gcccacgcgg tcgtcgacgc gatcgagcgc tacgtcgagg cgggcaaggg cctcacccgc
     5281 gacttaggcg gcaccgcgac caccgccgaa gtaggcaaag ccctcgccgc catgttctga
     5341 cggttgcggg ctaggccggc gcgcataggg gcgtggagaa gggtccagct gcgaggcggc
     5401 gcccgaaggg cgggcgcgag gcgtactccc tgtacgtgga gtgccccggc cgagggcgcc
     5461 aacgaagcag atgggccctt atccgcggcc cgcgccaacc gtcagaacat ggaggcgatc
     5521 gccttgccta cttcggtggt ggtcgcggtg ccgcctaggt cgcgggtgag acccttgccc
     5581 gcttcgacgt agcgctcgat cgcgtcgacg accgcgtggg ccgcctccgg gtggccgagg
     5641 tgatcgagca tcatcgcg
//
LOCUS       DATMCV010000219         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_413632, whole genome shotgun sequence.
ACCESSION   DATMCV010000219 DATMCV010000000
VERSION     DATMCV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413632"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1162)
                     /locus_tag="VNU03_05190"
     CDS             complement(5..1162)
                     /locus_tag="VNU03_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73883.1"
                     /translation="MNALALLFVLAFVVSVDLRIIAPVLPSIATSLHATAGAVGLAMT
                     SYAVAYGSGQLVYGPLSDRFGRVPVVRLAALGWSVCTALSALCATTWQFVAVRLLAGV
                     FAGSVIPLTLVYIGDTVEYARRQVVVGRFSIVTSAGLAFSAAIGGLVAHLLSWRAMLL
                     AYALVGLLPVALLWRLPTDRPARDPGDVEPVARFVDFLRDPFARTVYLSIFAEGFLLW
                     GPVTYLGAYAAARYGLNQLAVGLLIALFGVGTMVGGALMGSLRGRLSEGGLAWVGGLA
                     MGLALLIIAPRIGPLDSWVIFAAAMLLLGLGFVGLHTTLQVHATEISQAARGKAFSLF
                     PFSLFSGVAAGTAAMGPLVDAGGEHVMLAGCGLGLVGVGIVTARIRRRRSA"
     gene            complement(1167..>2004)
                     /locus_tag="VNU03_05195"
     CDS             complement(1167..>2004)
                     /locus_tag="VNU03_05195"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HWP73884.1"
                     /translation="GARYYTFAWTTVLTMRACARAGKPVIVLDRPNPLGGERLEGNWP
                     DGRFPSFVGLHPLPIRHGMTIAELAAYHNEMQEIGCDLTVVPMAGWRREMLWEDTELP
                     WVAPSPNMPTTDTARVYPGGCLLEGTNLSEGRGTTRPFEWVGAPYLDGPRLAAALERR
                     RIPGVRFRAASFEPAFHKWARRTCGGVQVHVTDWDRFQSFAAYLALIIEARRQAPRGF
                     RWKRPPYEFQWKKLPIDLLCGTDAIRLAIERGTPLARLEASWRPGLAAFARARRPYLL
                     YR"
BASE COUNT          332 a          713 c          756 g          203 t
ORIGIN      
        1 aggctcatgc gctcctcctg cgccggatgc gcgcggtgac gatgcccacc ccgaccaggc
       61 cgagcccgca gcccgccagc atcacgtgct cgccacccgc gtcgacgagc gggcccatcg
      121 cggcggtgcc cgcggccact cccgagaaca ggctgaacgg gaagagcgag aaggccttgc
      181 cccgcgcggc ctggctgatc tcggtggcgt ggacctgcag agtggtgtgc agacccacga
      241 accccaggcc gagcagcagc atggccgccg cgaagatgac ccacgagtcg agcggcccga
      301 tccgcggggc gatgatcagg agcgccaggc ccatcgcgag cccgcccacc caggcgagcc
      361 cgccctcgga gaggcgcccc cgcagggacc ccatgagcgc gccgcccacc atggtgccga
      421 ccccgaagag cgcgatcagc aggccgaccg ccaactggtt cagcccgtag cgcgcggccg
      481 cgtaggcgcc gaggtaggtc accgggcccc agagcaggaa gccttccgcg aagatcgaca
      541 ggtagacggt gcgggcgaac ggatcgcgca agaagtcgac gaagcgggcc accggctcca
      601 cgtcgccggg atcgcgcgcc gggcggtcgg tgggcagtcg ccagaggagc gccacgggca
      661 ggagccccac cagcgcgtag gcgagcagca tcgcgcgcca ggagagcagg tgggccacca
      721 gcccgccgat ggcggcggag aacgcgagcc cggcggaggt gacgatcgag aaccgtccca
      781 ccaccacctg ccgccgcgcg tactccaccg tgtcgccgat gtagacgagg gtcagcggga
      841 tgaccgagcc cgcgaagacc ccggccagca ggcgcaccgc cacgaactgc cacgtggtgg
      901 cgcagagcgc ggagagcgcg gtgcacacgc tccacccgag cgcggcgagc cgcaccaccg
      961 gcacgcggcc gaagcggtcg gagagcggac cgtagacgag ctgccccgag ccgtaggcga
     1021 ccgcgtagct cgtcatggcg aggccgacgg cgcccgcggt ggcgtgcagc gacgtcgcga
     1081 tggacggcag caccggggcg atgatgcgca gatccaccga gaccacgaag gccagcacga
     1141 acaggagggc gagcgcattc atccgctcat cgatagagca ggtagggccg ccgagcccgg
     1201 gcgaacgcgg ccaggccggg gcgccacgag gcttcgaggc gggcgagcgg cgtgccccgc
     1261 tcgatcgcga ggcggatcgc gtcggtgccg cagagcaggt cgatcgggag cttcttccac
     1321 tggaactcgt agggcggccg cttccagcgg aatccccgcg gggcctgccg gcgcgcctcg
     1381 atgatcaggg ccaggtacgc ggcgaaggac tggaagcggt cccagtcggt cacgtgcacc
     1441 tgcacgccgc cgcaggtgcg gcgggcccac ttgtggaacg cgggctcgaa ggaggcggcg
     1501 cggaagcgca cgccggggat ccgtcggcgc tcgagcgcgg cggccagccg cgggccgtcg
     1561 aggtagggcg cgccgaccca ctcgaagggc cgcgtggtgc cccggccctc ggagaggttg
     1621 gtgccctcga ggaggcagcc gcccgggtag acgcgcgcgg tgtcggtggt gggcatgttg
     1681 ggcgacggcg ccacccacgg cagctcggtg tcctcccaca gcatctcgcg ccgccagccc
     1741 gccatcggca ccaccgtgag atcgcagccg atctcctgca tctcgttgtg gtaggcggcc
     1801 agctcggcga tggtcatgcc gtgccggatc ggcaggggat ggagccccac gaaggacggg
     1861 aagcgcccgt cgggccagtt gccctcgagc cgctcgccgc ccagcgggtt cgggcggtcc
     1921 agcacgatca ccggcttgcc cgcgcgggcg caggcccgca tggtgagcac ggtggtccac
     1981 gcgaaggtgt agtagcgcgc gccg
//
LOCUS       DATMCV010000220         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_279282, whole genome shotgun sequence.
ACCESSION   DATMCV010000220 DATMCV010000000
VERSION     DATMCV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279282"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VNU03_05200"
     CDS             <1..477
                     /locus_tag="VNU03_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP73885.1"
                     /translation="QIPGRLELARAFGADHTINVKEVTDRRERVKLVRQWTGGVGADV
                     ACDLVGFPAVIPEGIEMLRSGGTYLEIGTISRGAKIELEPSLLVWGSKKIVGVIQYEA
                     WVIPRALDFLVRTRERYPWSRILSHKYPLEQINEAFAASEWHNRETTTITRAALTP"
     gene            474..1310
                     /locus_tag="VNU03_05205"
     CDS             474..1310
                     /locus_tag="VNU03_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWP73886.1"
                     /translation="MSRTIADLIQDRFGLPTEAGRARPAEGPLALLLSHRTQRRYKPD
                     PVPDDVLEMALAAALSAPSKSDLQQVGIVVVRDRSKQSTIGGWIPDMPWIAQAPRFMV
                     FCGDHRRIRRVSELRGRPFPNDTLDMFMNAAVDTGLALQAFITAAEALGLGCCPISVV
                     RNHVEKLAALLELPPGVFPVAGLCVGYPLQAGFISLRLPPAITVHTDRYDDGELPAQL
                     AAYDQRREARHATPKENQRYVERFGYTEPYGWSEDKARQYSTPERHSFGPFIRGHGFG
                     LA"
     gene            complement(1307..1636)
                     /locus_tag="VNU03_05210"
     CDS             complement(1307..1636)
                     /locus_tag="VNU03_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73887.1"
                     /translation="MAENFVILVVIAALAGGFALMLFTAMWRVERRAQFGREAGIKRS
                     QRLERRVFLIGVVLVVAMVTVVVTVQLSRAPGCRGTLVMVAGSDGRPLECACEQGRRG
                     ACFDPGP"
     gene            complement(1755..2375)
                     /locus_tag="VNU03_05215"
     CDS             complement(1755..2375)
                     /locus_tag="VNU03_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWP73888.1"
                     /translation="MASELRVLVADDDAIARRFLESTLRKAGYAVTVASSGDEAWRIL
                     SSQDVPTIAILDWMMPGLTGVEICEKVRAASLPMAPYLIVLTSRSEPGDVVEALRAGA
                     DDHITKPFEIAELRARLAVGVRIVTLQQQLTERVHSLEEALAHVQQLQGLIPICAWCR
                     QVRNDGNFWEQVDSYLTKRSGLQFTHAICPTCRDREVAHARDSRPT"
     gene            2449..2802
                     /locus_tag="VNU03_05220"
     CDS             2449..2802
                     /locus_tag="VNU03_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWP73889.1"
                     /translation="MTESGPLIDLDALLASVGGDRELLDELAATFIAEVPGWIATLRA
                     ALKNREPETVFRVAHGVSGSLGYFRAAGVRKRASDLEAMGRAGRLDDAAIEIDRLEAD
                     LHALSRVLGEAPWRR"
BASE COUNT          412 a         1030 c          970 g          392 t
ORIGIN      
        1 cagatcccgg ggcggctcga gctggcccgc gccttcgggg ccgaccacac catcaacgtc
       61 aaggaggtca cggaccggcg cgagcgggtc aagctggtac gccagtggac cggcggggtc
      121 ggggccgacg tggcgtgcga cctggtgggc tttcccgcgg tgatcccgga gggcatcgag
      181 atgctgcgct cgggcggcac ctatctcgag atcggcacca tcagccgcgg ggccaagatc
      241 gagctcgagc cgtcgctcct ggtgtggggc tccaagaaga tcgtcggggt gatccagtac
      301 gaggcgtggg tgatcccgcg cgcgctcgac ttcctggtgc gcacccgcga gcgctatccg
      361 tggagccgca tcctctcgca caagtacccg ctcgagcaga tcaacgaggc cttcgcggcc
      421 tccgagtggc acaaccgcga gaccaccacg atcacccggg cggcgctcac cccgtgagcc
      481 ggacgatcgc ggatctgatc caggaccgct tcggcctgcc caccgaggcc ggtcgcgcgc
      541 ggcccgcgga gggcccgctc gccctgctgc tctcgcatcg cacccagcgc cgctacaagc
      601 ccgatccggt gcccgacgac gtgctcgaga tggcgctggc cgccgcgctc tccgcgccgt
      661 cgaagtccga cctccagcag gtgggcatcg tcgtcgtgcg cgaccgcagc aagcagtcga
      721 ccatcggcgg gtggatcccc gacatgccgt ggatcgcgca ggcgccgcgg ttcatggtgt
      781 tctgcggcga ccaccgacgg atccgccggg tgagcgagct gcgcggccgc cccttcccca
      841 acgacaccct cgacatgttc atgaacgcgg cggtggacac cggcctcgcg ctgcaggcct
      901 tcatcaccgc cgcggaggcc ctggggctcg gctgttgccc gatcagcgtg gtgcgcaacc
      961 acgtcgagaa gctcgccgcc ctcctcgagc tgccgcccgg ggtgttcccg gtggccggcc
     1021 tctgcgtcgg ctacccgctg caggcgggct tcatcagcct gcgcctaccg cccgcgatca
     1081 cggtgcacac cgaccgctac gacgacgggg agctgcccgc ccagctcgcg gcctacgatc
     1141 agcggcgcga ggcgcgccac gccaccccga aggagaacca gcgctacgtg gagcggttcg
     1201 gctacaccga gccgtacggc tggtcggagg acaaggcccg ccagtactcg acgccggagc
     1261 gacacagctt cggcccgttc atccgcggtc acggcttcgg gctcgcctag ggcccgggat
     1321 cgaagcaggc cccgcgccgg ccctgctcgc acgcgcattc gagcgggcgg ccgtcggacc
     1381 ccgccaccat caccagcgtc ccgcggcagc cgggggcccg ggagagctgc acggtcacca
     1441 ccaccgtgac catcgcgacc accagcacca ccccgatcag gaagacccgt cgctcgaggc
     1501 gctgactccg cttgatcccc gcctcgcgtc caaactgcgc gcgccgctcg acccgccaca
     1561 tggcggtgaa cagcatgagg gcgaacccgc ccgcgagcgc ggcgatgacg acgaggatga
     1621 cgaagttttc ggccactggg atcgggtgtc gacgagaacg ttgtcgcggc agcgggccgt
     1681 cgtcggcccg gcgcggccat cgacccggcg cactgtagca cgatcgcggg gcgccggaaa
     1741 cccgccccgc ggcgctaggt cgggcgtgaa tcccgcgcat gcgccacctc ccggtcccgg
     1801 caggtggggc agatggcgtg ggtgaactgc agccccgagc gcttggtcag gtagctgtcc
     1861 acctgctccc agaagtttcc gtcgttgcgc acctggcggc accaggcgca gatcggaatg
     1921 aggccctgga gctgctgcac gtgggccagc gcctcctcca gcgagtggac tctctcggtg
     1981 agctgctgct ggagcgtgac gatccgcacc ccgaccgcga gacgggcccg cagctcggcg
     2041 atctcgaagg gcttcgtgat gtgatcgtcg gccccggccc ggagcgcctc cacgacgtcc
     2101 ccgggctcgc tccgggacgt cagcacgatc aggtagggcg ccatcggcag gctggccgcg
     2161 cgcaccttct cgcagatctc gaccccggtg aggccgggca tcatccagtc gaggatcgcg
     2221 atggtgggga cgtcctggct gctcaggatt cgccacgcct cgtccccgct cgaggccacg
     2281 gtcaccgcgt agcccgcctt gcgcagggtg ctctcgagga accgccgcgc gatcgcgtcg
     2341 tcatcggcga ccagaacccg gagctcgctg gccatggatt cattctgccc cagaacgccc
     2401 gcttccaggc cgaggggtgg gcggcgggtg gagtacgatg gcccggtcat gaccgagtcc
     2461 ggccccttga tcgatctcga tgccctgctg gcaagcgtcg gcggcgaccg cgagctgctc
     2521 gacgagctgg ccgccacctt catcgcggag gtgcccggct ggatcgccac gctccgggcc
     2581 gccctgaaga accgtgagcc cgagaccgtc ttccgggtgg ctcacggcgt gagcggctcg
     2641 ctgggctact tcagggcggc gggggtccgg aagcgagcgt ccgacctcga ggcgatgggg
     2701 cgagccggcc gtctcgacga cgcggccatc gagatcgatc ggctcgaagc cgatctgcac
     2761 gccttgagcc gcgtcctcgg cgaggccccc tggcgacgct gagc
//
LOCUS       DATMCV010000221         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_146812, whole genome shotgun sequence.
ACCESSION   DATMCV010000221 DATMCV010000000
VERSION     DATMCV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146812"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..693)
                     /locus_tag="VNU03_05225"
     CDS             complement(169..693)
                     /locus_tag="VNU03_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HWP73890.1"
                     /translation="MTVRAHRHLKWPAFDYLERGLMVLCALLLLGFTVCEVADVLFRN
                     LGRPWLNANEFAGGLFTWGVFLGMGVAVRRDQHFRLTAVGEALSGRKRMLVETFNRLV
                     ILGVGLCMVWFGYKNYLNGFGSFLMPSVTPIAVLYAAIPVAGFLVALFTLEELINGWR
                     HGFPARSRVTAPPT"
     gene            complement(743..1753)
                     /locus_tag="VNU03_05230"
     CDS             complement(743..1753)
                     /locus_tag="VNU03_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWP73891.1"
                     /translation="MLTRRSFLARTTTLAVGAAAGTPRRARAQESKTFTFGYDQPRDT
                     AYSNLADFFEKKLLELSGGSLKIRQFPSAALGQEPEMAQKVRAGDIDFAINATANTAT
                     VVPQSGVFSLHFIFRDEAHLEKAMADRAINDAFRQMINDNTTGARSLGLVTQGFRNMY
                     AKFAVSSVNDIVGKKVRVQATKTEDAFFSAYHAIPVHMPFGQVYTSLQTGLIHIAENG
                     NDVVLKNKHYEAAPFVSKTEHEANNSHIWVSQKTWDDLNETQRKQIQQAADYALPAAS
                     RKALELSTSAIGSLQKVGVKFHEQVDKKSFSTIAAPLQDSQAQELGKHAVQLLALVRG
                     IK"
     gene            complement(1854..2210)
                     /locus_tag="VNU03_05235"
     CDS             complement(1854..2210)
                     /locus_tag="VNU03_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP73892.1"
                     /translation="MPRFATLGVLLTLVSLTGGKTLAQAPPGQIEEMVLLNNDSVRLV
                     LITYAPGADSDLHLNVGPEVTIVQEGELVMFAQGRREVLHPGEAHWLPDATTHLARNE
                     GDRPVKFWSLLLKRCD"
     gene            complement(2222..2668)
                     /locus_tag="VNU03_05240"
     CDS             complement(2222..2668)
                     /locus_tag="VNU03_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWP73893.1"
                     /translation="MVVATCSMAVKPQARRLAECALAAVWLLLAATPGGAAHGVPVAV
                     VTTAPEYAKRLLDGGQPIVFVDLRPREDFAHGRLPHALSVPLPELRRRYHEISRTELV
                     ILYCDCAPEALAAAFRFVTGEGYQNASVLAEGFRGWVKRGYPVEPR"
     gene            2761..>2948
                     /locus_tag="VNU03_05245"
     CDS             2761..>2948
                     /locus_tag="VNU03_05245"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWP73894.1"
                     /translation="MPTFMVQASYTSPAWSKLVQRPENRMEALRPVVEKLGGEIIAWY
                     YAFGDYDVVVLFDVPSNV"
BASE COUNT          469 a          925 c         1095 g          459 t
ORIGIN      
        1 cgttgaacat ctgcccgacc atcgacgcga gcgagatctt ggtgaagaag gcggtggtga
       61 cgaacgcgcc cgcggccagg gcgaaggcca ccgggacccc ctggtagccc aggtagagga
      121 aggtcccgac catgacgatg atcgcgagcg agttcgagtc gagatgggtc atgtgggtgg
      181 tgcggtcacc cgggagcggg cggggaagcc gtggcgccag ccgttgatca gctcctcgag
      241 ggtgaagagg gcgaccagga agccggccac cggaatggcg gcgtaaagga ccgcgatcgg
      301 cgtgaccgag ggcatcagga agctgccgaa gccgttcagg tagttcttgt agccgaacca
      361 gaccatgcag aggcccacgc ccaggatgac cagccggttg aacgtctcga ccagcatgcg
      421 cttgcgcccg gagagcgcct cgccgaccgc ggtgagccgg aagtgctggt cgcgccggac
      481 cgcgaccccc atgccgagga agacgcccca ggtgaagagc ccgcccgcga actcgttggc
      541 gttgagccac gggcggccga ggttgcggaa gaggacgtcg gccacctcgc agaccgtgaa
      601 gcccaggagc agcagggcgc agaggaccat cagcccgcgc tccaggtagt cgaaggcggg
      661 ccacttgagg tgacggtgcg cgcgcaccgt catgcgctcg gatggcgggg acgcgccgcc
      721 cggggccggc gacgccgcca cgctacttga tgccgcgaac cagggccagc agctggacgg
      781 cgtgcttgcc gagctcctga gcctgcgaat cctgaagggg cgccgcgatc gtgctgaagc
      841 tcttcttgtc gacctgctcg tggaacttga cccccacctt ctgcagcgag ccgatcgccg
      901 aggtggagag ctcgagggcc ttgcggctgg ccgcgggcaa cgcgtagtcg gccgcctgct
      961 ggatctgctt gcgctgggtc tcgttcaggt cgtcccaggt cttctggctg acccagatgt
     1021 gggagttgtt ggcctcgtgc tcggtcttgg agacgaacgg cgcggcctcg tagtgcttgt
     1081 tcttcagcac cacgtcgttg ccgttctccg cgatgtggat gagcccggtc tggagcgacg
     1141 tgtagacctg gccgaacggc atgtgcaccg ggatggcgtg gtaggcgctg aagaaggcgt
     1201 cctcggtctt ggtggcctgc acccgcacct tcttgcccac gatgtcgttg acgctcgaga
     1261 ccgcgaactt ggcgtacatg ttgcggaagc cctgggtgac gaggccgagg gagcgggcgc
     1321 cggtggtgtt gtcgttgatc atctgccgga aggcgtcgtt gatcgcgcgg tcggccatcg
     1381 ccttctcgag atgggcctcg tcgcggaaga tgaagtgcag ggagaagacg ccggactgcg
     1441 gcaccacggt ggcggtgttg gcggtggcgt tgatcgcgaa gtcgatgtcg ccggcccgca
     1501 ccttctgggc catctccggc tcctggccga gggcggcgct cgggaactgc cggatcttga
     1561 gcgagccgcc cgagagctcg agcagcttct tctcgaagaa gtcggccagg ttgctgtacg
     1621 cggtgtcgcg gggctggtcg tagccgaacg tgaaggtctt cgattcctgg gcgcgggcgc
     1681 ggcgtggcgt ccccgccgcg gcgccgacgg ccagggtcgt ggtgcgcgcc aggaacgagc
     1741 gtctcgtcag catggtcgac ctcctcgtcg acagcgggtg tcgtgatggg cgggcaaaca
     1801 ctagccctgc ggggcccggc gagtcaacgg ctattccggc cgtggcgcgg gcgtcagtcg
     1861 cagcgcttga gcagcaacga ccagaacttg accgggcgat cgccctcgtt gcgggccagg
     1921 tgggtggtcg cgtcgggaag ccagtgggct tcgcccggat ggagcacctc gcgccggccc
     1981 tgcgcgaaca tcaccagctc gccttcctgg acgatcgtga cctcggggcc gacgttcaga
     2041 tgcaggtcgg agtcggcgcc gggcgcgtag gtgatgagca ccagccggac cgagtcgttg
     2101 ttcaggagga ccatctcctc gatctggccc ggcggcgcct gggccagggt cttcccgccg
     2161 gtgagcgaga ccagcgtgag caggacaccg agggtggcga agcgcggcat ggacgctctc
     2221 ctcatcgcgg ctccaccggg tatccgcgct tgacccaccc gcggaatccc tcggccagga
     2281 cgctcgcgtt ctggtagccc tcgccggtca cgaatcggaa cgcggcggcg agggcctcgg
     2341 gggcacagtc acagtacagg atgaccagct cggtccggga gatctcgtgg tagcgccggc
     2401 gcagctcggg caggggaacc gagagggcgt ggggcagccg gccgtgcgcg aagtcctccc
     2461 gcggtctcag atcgacgaag acgatcggct ggcccccgtc gaggagacgc ttggcgtact
     2521 cgggcgcggt ggtgacgacc gcaaccggga cgccgtgtgc agccccgccg ggcgtggcgg
     2581 cgagcagcag ccagaccgcg gcgagcgcgc actccgcgag cctccgcgcc tggggcttca
     2641 ccgccatgga acacgtcgcg acgaccatca gttcccggta gcgtagccga tcccggctgg
     2701 agtcgccgcg cgggcgcggc gggggtatat ttccaggaaa cggcggcgag gaggaccccg
     2761 atgcccacat tcatggtgca ggcgtcctac accagtccgg cgtggagcaa gctcgtccag
     2821 cggcccgaga accggatgga ggcgctccgg ccggtggtgg agaagctggg cggcgagatc
     2881 atcgcgtggt actacgcctt cggcgactac gacgtggtcg tgctcttcga tgtgccgagc
     2941 aacgtcaa
//
LOCUS       DATMCV010000222         3670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_612692, whole genome shotgun sequence.
ACCESSION   DATMCV010000222 DATMCV010000000
VERSION     DATMCV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612692"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VNU03_05250"
     CDS             <1..623
                     /locus_tag="VNU03_05250"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWP73895.1"
                     /translation="GAWAQPRELPAQLVTLGGVAELHRKDTPAWAPAALRAQVGEGDS
                     LRTQLGGRVAIRTASGQALRLGSRSQIGVMASDGDATTPGPTRVRLDSGWLWVAVMPG
                     SPAPTQIEVRAGPTVVTIRGAGVGLRRAADGALLVQVHHGGAVCAGPDRQWERTLTGP
                     QQLLVPASGVPAQPVALAVDPLETTWVRWNAEQDLAGGYGGNTPKP"
     gene            631..1473
                     /locus_tag="VNU03_05255"
     CDS             631..1473
                     /locus_tag="VNU03_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP73896.1"
                     /translation="MSLDALVVGTELPEYRVSATPTHDAWENKIHEDGLAREFGFRGG
                     LVPGVTVYAWMTRPVVAALGGAWLDHGRFSVRFAKPVYFGQPITVRTSVAAHSKDEVT
                     IQARVVNDEDEVCATATMGLPLGPLLALPELAAYPVAPLPVERPVATRAHLAGHPILG
                     SPELLLERSVIDAFLTRVSDTLPVYRDADAPAHPGLYLDQANRALDRNVRVSPWIHVE
                     SHGQHLTVTRLGERLATRAKVKNLFQGKGHEFVELDLLLVAEGTRPVASIRHVAIYQL
                     RATG"
     gene            1478..2869
                     /locus_tag="VNU03_05260"
     CDS             1478..2869
                     /locus_tag="VNU03_05260"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP73897.1"
                     /translation="MIKILVVDDEAEIRSLLAAVLQNKGYEVVTAEDGAAALQAVPRE
                     RPAVILMDLSMPRMNGMDALTEIKRLDAEVPVIICTAHADFATAVRAMKLGAYDYLTK
                     PFDVELLILTLERAVERHRLHSRIEELKRQGQGSSLAERMGGSPAIAQVIQQVSQVAE
                     SNFTVLVQGETGTGKELVARGIHQQSPRRPAPFVAVDCGAIPETLVESELFGHERGAF
                     TGAQARREGHFQLAKGGTLFLDEIGNVPLATQAKLLRALEQREVNPLGATRAVAVDAR
                     IIAATNSELEESVKAGRFRADLYYRLSEFTIALPPLRSRREDIMHLSQRFLDEVSMEL
                     RRPVRRIADEAMQVLLHHDWPGNVRELRNVVRKAALLATDVVTPEHIPALSASAPAPS
                     RAAAEPLGEDLSLREVAELAAGQAEREVIRHALESTKGNKSQAARLLRTDYTTLHAKM
                     KRYGISARDFTPR"
     gene            3098..3424
                     /locus_tag="VNU03_05265"
     CDS             3098..3424
                     /locus_tag="VNU03_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP73898.1"
                     /translation="MNTLEAAPLWMSVPDLVAPSQPAVEAQRPPRPDAPAESRVPMRV
                     GLFSLRQIVDSAVTRAEAEAIRRALASTLGNKSHAARLLRTNYTTLHGKMKRYGISAR
                     EFQRSA"
BASE COUNT          530 a         1314 c         1344 g          482 t
ORIGIN      
        1 ccggagcgtg ggcgcagccg cgcgagctgc ccgcccagct cgtgacgctc ggcggtgtcg
       61 ccgagctgca ccggaaggat acgcccgcgt gggcgccggc cgccctgcgc gcccaagtgg
      121 gagaaggcga cagcctccgc acccagctgg gcgggcgggt cgccatccgg accgccagcg
      181 gtcaggcgct gcggctcggg tcccgctcgc agatcggggt gatggcctcc gacggagacg
      241 ccaccacgcc gggccccacc cgggtgcggc tcgacagcgg atggctctgg gtcgcggtca
      301 tgccgggtag tcccgcgccg acgcagatcg aggtccgggc cgggcccacc gtggtcacca
      361 tccgcggcgc cggggtcggg ctcaggcgcg cggcggacgg cgcccttctc gtgcaggtcc
      421 accacggggg cgcagtatgc gcggggccgg accggcagtg ggagcggacg ctgaccggcc
      481 cgcagcagct cctcgtcccg gcctcgggcg tgcccgcgca gccggtcgcg ctcgcggtgg
      541 atcccctcga gacgacctgg gtccggtgga acgcggagca ggatctcgcc ggaggttacg
      601 gcggcaacac gcccaagccc tgaggacggg atgagcctcg acgccctcgt cgtggggacc
      661 gagctcccgg agtaccgggt gagcgccacc ccgacgcacg acgcgtggga gaacaagatc
      721 cacgaggacg gactcgcccg cgagttcggg tttcgcggcg ggctggtgcc cggggtgacc
      781 gtctacgcgt ggatgacgcg cccggtggtg gccgcgctcg gcggcgcgtg gctcgatcac
      841 gggcggttct cggttcgctt cgccaagccg gtctacttcg ggcagccgat cacggtgcgc
      901 acgagcgtgg ccgcgcactc caaggacgag gtcacaatcc aggcccgggt ggtgaacgac
      961 gaggacgagg tgtgcgccac cgcgacgatg gggctgccgc tggggccgct actcgcgctg
     1021 ccggagctgg ccgcctatcc cgtcgcgccg ctccccgtcg agcgaccggt ggcgacccgg
     1081 gcccacctcg caggccaccc gatcctgggc tcgcccgagc tgctgctcga gcggtcggtg
     1141 atcgacgcgt tcctcacgcg cgtcagcgac acgctgccgg tctaccggga cgccgacgct
     1201 cccgcgcacc ccgggctcta cctcgatcag gccaaccggg cgctggaccg caacgtgcgg
     1261 gtgagcccat ggatccacgt ggagagccac ggccagcacc tcaccgtgac ccggctgggg
     1321 gagcgtctgg ccacgcgcgc caaggtgaag aacctcttcc agggcaaggg gcacgagttc
     1381 gtggagctgg acctgctcct ggtggcggag gggacgcggc cggtggcctc gatccgtcac
     1441 gtcgccatct accagctccg ggccacggga tgatccggtg atcaagatcc tggtggtcga
     1501 cgacgaggcc gagatccgct cgctcctcgc ggcggtcctc cagaacaagg gctacgaggt
     1561 ggtgaccgcc gaggacgggg ccgccgcgct ccaggcggtg ccgcgcgagc gccccgcggt
     1621 gatcctgatg gacctgagca tgccgcgcat gaacggcatg gacgcgctga ccgagatcaa
     1681 gcgcctcgac gcggaggtcc cggtcatcat ctgcaccgcc cacgccgact tcgccaccgc
     1741 ggtgcgggcg atgaagctgg gagcctacga ctacctgacc aagcccttcg acgtggagct
     1801 gctgatcctc accctcgagc gcgcggtgga gcgccaccgg ctgcactcgc gcatcgagga
     1861 gctgaagcgg caggggcagg gcagctcact ggccgagcgc atgggcggca gcccggcgat
     1921 cgcgcaggtg atccagcagg tgtcccaggt ggcggagtcg aacttcacgg tgctcgtcca
     1981 gggcgagacc gggaccggca aggagctggt ggcccgcggg atccaccagc agagcccgcg
     2041 ccgaccggcg ccgttcgtgg cggtcgactg cggcgcgatc ccggagaccc tggtggagtc
     2101 ggagctgttc gggcacgagc ggggggcgtt caccggggcg caggcccgcc gcgagggcca
     2161 cttccagctg gccaagggcg ggaccttgtt cctcgacgag atcggcaacg tgccgctcgc
     2221 cacccaggcc aagctcctgc gggcgctcga gcagcgcgag gtcaacccgc tcggcgccac
     2281 gcgggcggtg gcggtggacg cgcgcatcat cgcggcgacg aactcggagc tcgaggagag
     2341 cgtgaaggcg gggcggttcc gcgccgacct gtactatcgc ctcagcgagt tcacgatcgc
     2401 actgcccccg ctgcggagcc ggcgggagga catcatgcac ctctcgcagc gcttcctcga
     2461 cgaggtgagc atggagctgc ggcgtccggt gcggcggatc gcggacgagg cgatgcaggt
     2521 gctgctgcac cacgactggc ccggcaatgt gcgcgagctg cgcaacgtgg tgcgcaaggc
     2581 cgcgctgctc gcgaccgacg tggtgacgcc cgagcatatt cccgcgctca gcgcatccgc
     2641 ccccgcgcca tctcgcgcgg ccgccgagcc gctgggcgag gatctctcgc tccgcgaggt
     2701 cgccgagctg gccgcgggcc aggccgagcg cgaggtcatt cgccacgcgc tcgagtcgac
     2761 caagggcaac aagagtcagg ccgcgcgcct gctccggacc gactacacca cgctccacgc
     2821 gaagatgaag cgttacggca tctccgcccg ggacttcacg ccccgctagg cccgcgcccg
     2881 ccctgaccgt ctccctatgg cgccgccgcc atagctctgg tgccgctccg ctattgcccg
     2941 tggtttccac ccgccgcccc cagccccgcc gatcacgtaa gtagcggaga tcgttcgcgc
     3001 ctgtcactcg accacgagtc gagcgccatg gcactgcgct tgccctgcta tcagtgcggt
     3061 ttcgtaccac gcgaccacca ctgaaggaga gcaagccatg aacactctcg aggccgcccc
     3121 gctatggatg tccgtccccg atctcgtggc tccgtcccag cccgccgtcg aggcgcagcg
     3181 gccgccacga cccgatgcgc cggcggagag tcgcgtcccg atgcgcgtcg ggctcttctc
     3241 gctccggcag atcgtcgatt cggcggtgac ccgggcggag gcggaggcga tccgtcgcgc
     3301 gctggccagc acgctcggca acaagagcca cgcggcgcgg ttgctgcgca cgaactacac
     3361 cacgctgcac ggaaagatga agcggtacgg gatctccgcg cgggagttcc agcgctccgc
     3421 gtagcgcgcg agagcgagct ctggcggacc agcacgtgac catgggtggt ggtcgaggac
     3481 cgttgcgcgg ggcgccgacc ggtgccgttg cggacccggc ggcgccgggc gcatgggccg
     3541 ggggactggg ccgggttctg ctcgacgcgc tccccgcgat gaccgtgatc gtcgacgacc
     3601 ggggcgtcat cgcggccgcg aacgaggcgt ggcggcgctt cggccgggag aacggcgccg
     3661 gccccgcggt
//
LOCUS       DATMCV010000223         5562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_544662, whole genome shotgun sequence.
ACCESSION   DATMCV010000223 DATMCV010000000
VERSION     DATMCV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544662"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1063)
                     /locus_tag="VNU03_05270"
     CDS             complement(179..1063)
                     /locus_tag="VNU03_05270"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWP73899.1"
                     /translation="MKPLGDPDALISTDDLAARLGQPGLRVYDCTTYLEPPDPGSDEP
                     YKAVAGLKTFVAGHIPGADFLDLQGEFSATDTKLKFMMPPAEPLARAFGRHGLGDGAR
                     VVLYSVGTMMWATRFWWMLRTLGFDGAAVLDGGFDRWQAEGRPIETGDPKGYPRATFT
                     PRPRPGRFVDKTAVKAAIGDPRAVTVNALGPQFHRGLEPSRYGRPGRVPGSVNVPAAD
                     LIDPATKRFVSPAAALASFAAAGIDKDRRVIAYCGGGISATIDLFLLHQLGFQDLTLY
                     DGSMGEWAKDPSLPIETD"
     gene            1144..2514
                     /locus_tag="VNU03_05275"
     CDS             1144..2514
                     /locus_tag="VNU03_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWP73900.1"
                     /translation="MATTTPVKSPQALAETRRWAAPGSKSAALFAQAQGVMPGGNTRT
                     TVYMAPYPPYAASGDGCWITDVEGDRRLDCLNNYTALIHGHAHAALVEAATRRLALGS
                     SFPLPTPEEVELATLLCERLPSAERVRFTNSGSEAVMMAIKGARAWTGRPKIAKFEGA
                     YHGSYDYAEVSLSSAPEQWGSLAAPASTAYSRGVPQAVLDDVVVLPFNHAELAVARIE
                     REAKHLAAVLVDPIPNRVGLIPAQRDFLGALRGVTTAHGIALIFDEVITFRVGYRGAQ
                     GAFGVTPDMTTLGKIIGGGFPVGAVAGRAEVMAVFDPTGGKPAAPHGGTFNANPVTMA
                     AGLAAMRLLTPEAFARLDELGAKLRGSLEACFKHAGVPGAVTGQGSLFRVHATDRELV
                     DYRSTRTTPEEEARLQRLIRGLMERGVLMSITGLGCLSTPMGDAELESLTETFAAVLA
                     AERGPR"
     gene            2511..3923
                     /locus_tag="VNU03_05280"
     CDS             2511..3923
                     /locus_tag="VNU03_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP73901.1"
                     /translation="MTRGGDVHQTDALTLARQIRTKQVSPVEAVEATLRRIDALQPTV
                     NAFITVTADEARDAARRAEAAVMAGHRLGPLHGVPFSVKDLLFTTGVRTTMGSLIFAD
                     QVPVADAVPVRRLREAGAILIGKTTTPEFGHKPLTDSPLFGATRNPWDLSRTAGGSSG
                     GAAAAVATGQGPLALGTDGGGSVRLPASCCGIVGLKPTLGRVPHTQQADLFSSTSYIG
                     PMARSVAETAACFDAIVGFDPGDPYSRPEPPDDPRDVAVRGLRLGWLPRVGNRLVDPE
                     VLAACEAAVRHLEGQGAHVETLEEDVSAYERTFLIGLQAGLTARVGGHMAKFGDKVAP
                     SLRESIERGAQWSAVDWVTALGQRTAMYRRVNALLQRFDFLLSPTLSRPALAVDHDAF
                     EPITIAGEPAGSIRGAWYPYLWPFNLSGHPAVSLPCGWSSDGLPIGLQIVGPWYGDRR
                     VLALAGHLERERPCARPMPL"
     gene            3951..4775
                     /locus_tag="VNU03_05285"
     CDS             3951..4775
                     /locus_tag="VNU03_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HWP73902.1"
                     /translation="MPLSPKGFPVLLSFDIDAETMWTGRDPKNAERPIVMSQGAYGWK
                     VGMPRILDLLARYGLRVTFFVPGLVMEQRPAMVEAILKAGHEIAHHSWSHAWIVNLTL
                     EQEREEMDRGIEIIKRMTGRNPVGYRSPAAEFSPHTLKMLAEYKFGYSSNYFDDDSPY
                     LHRIDGKLTDIVEFPFAWVLDDAPFFQYSITLPGRTMQAPSAVFEAWRREFDVLYRED
                     RYFCLAMHPQIIGRPSRITLLEELIRYILGHPRVWLARCDEAADALRPALRAEARA"
     gene            4772..5515
                     /locus_tag="VNU03_05290"
     CDS             4772..5515
                     /locus_tag="VNU03_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP73903.1"
                     /translation="MRLAGRRVLVTGAASGIGLAIARLFRSEGAAVAMLDRDEAGLAK
                     AQVDGAATLVCDVAEERQVRAAVAKAATALGGLDGVVNSAGIDLMRPFERMTSEEWAR
                     IMAVDLTGPMLVCHAALPALKQAGRGTIVNIASGAALRPLEQRTAYCTAKAGLVMFGK
                     TLAVDLAADHIRVNAICPGIIDTPLFRSSWEGAPDPEAELARILDRYVIKRPGEPEEI
                     AQAALYLTSDESSFVTGAALAVDGGRTFH"
BASE COUNT          747 a         2051 c         1990 g          774 t
ORIGIN      
        1 cggcggaacc ccgccgggag tcgaggcgac ctattatccg ctgcgcccag cggaggcggt
       61 ggtcccgagg gacaccgact ggcggaccct cctcgcctcc ggcgtgccgc ggagcgatgt
      121 gctgcagcgg gcgtagccgg aatctacgag gccgcgcgcc tcggcgacgg agccgacctc
      181 agtcggtctc gatcggcagg ctcgggtcct tggcccactc gcccatggag ccgtcgtaga
      241 gcgtgaggtc ctggaagccg agctggtgca gcaggaagag gtcgatggtc gcggagatgc
      301 cgcccccgca gtaggcgatc acccgccggt ccttgtcgat gccggcggcg gcgaagctgg
      361 ccagcgcggc ggcgggcgag acgaagcgct tggtcgcggg atcgatcagg tcggccgccg
      421 gcacgttgac cgagcccggc acgcggccgg gccggccgta gcgcgagggc tccaggccgc
      481 ggtggaactg ggggccaagc gcgttgacgg tgaccgcgcg gggatcgccg atcgcggcct
      541 tcaccgcggt cttgtcgacg aagcgccccg ggcgtggccg tggcgtgaag gtcgcccgcg
      601 gatagccctt ggggtcgccc gtctcgatcg gccggccctc cgcctgccac cggtcgaagc
      661 cgccgtcgag caccgcggcg ccgtcgaagc cgagcgtgcg gagcatccac cagaagcgcg
      721 tcgcccacat catggtgccg acgctgtaga gcacgacgcg ggcgccgtcg ccgagcccgt
      781 gccggccgaa ggcgcgcgcc agcggctcgg cgggcggcat catgaacttg agcttcgtgt
      841 cggtcgcgga gaattccccc tgcaggtcga ggaagtcggc ccccgggatg tgcccggcca
      901 cgaaggtctt caagcccgcg accgccttgt agggctcgtc cgagccggga tcgggtggct
      961 cgagatacgt cgtgcagtcg tagacacgta gcccgggctg gccgagccgg gcggccaggt
     1021 cgtcggtcga gatcagcgcg tcgggatcgc ccagaggctt catatggggc cgagtctgcc
     1081 acgagaggca cgggccgtcc agagccggac cgcctcgact ataatcaccg ccggaggatc
     1141 cccatggcca cgaccacccc cgtgaagtcc ccgcaggcgc tcgccgagac ccgacgctgg
     1201 gctgcgcccg gctcgaagtc ggccgcgctc ttcgcgcagg cccagggcgt gatgccgggc
     1261 ggcaacacgc gcaccaccgt ctacatggcg ccctacccgc cctacgcggc gtcgggcgac
     1321 ggctgctgga tcacggacgt ggagggcgat cgccggctcg actgcctgaa caactacacc
     1381 gcgctgatcc acgggcacgc acacgcggcg ctcgtcgagg cggccacgcg gcggctcgcg
     1441 ctgggctcgt cgttcccgct gcccacgccg gaggaggtcg agctggccac gctcctctgc
     1501 gagcgcctgc cctcggccga gcgcgtgcgc ttcaccaact ccgggagcga ggcggtgatg
     1561 atggcgatca agggcgcgcg agcctggacg gggcggccca agatcgccaa gttcgagggc
     1621 gcctaccacg gctcctacga ctacgcggag gtgagcctct cctccgcccc cgagcagtgg
     1681 ggcagcctcg ccgcgccggc cagcaccgcg tattcgcgcg gcgtgccgca ggcggtgctc
     1741 gatgacgtgg tggtgctgcc gttcaaccac gccgagctgg ccgtcgcgcg tatcgagcgc
     1801 gaggccaagc acctcgccgc ggtcctggtc gatccgatcc cgaaccgcgt cggtctgatc
     1861 cccgcgcagc gcgacttcct gggcgccctc cgcggggtga ccaccgccca cggcatcgcg
     1921 ctgatcttcg acgaggtcat caccttccgc gtgggctacc ggggcgccca gggcgcgttc
     1981 ggcgtgacgc cggacatgac gacgctcggc aagatcatcg gcggcggctt cccggtgggc
     2041 gcggtggcgg gccgggccga ggtgatggcg gtgttcgatc ccaccggcgg caagccggcg
     2101 gcgccgcacg gcggcacgtt caatgccaac ccggtcacca tggccgcggg cctggccgcg
     2161 atgcgcctgc tgacgcccga ggccttcgcc cggctcgacg agctgggggc caagctgcgc
     2221 ggcagcctcg aggcctgctt caagcacgcc ggggtgcccg gcgcggtcac cggccagggc
     2281 tcgctcttcc gcgtgcacgc gaccgatcgc gagctggtcg actaccgcag cacgcgcacc
     2341 acgcccgagg aggaggcgcg gctgcagcgc ctcatccgcg ggctgatgga gcgcggggtg
     2401 ctgatgtcga tcaccgggct cggttgcctc tccacgccga tgggcgacgc ggagctggag
     2461 agcctgaccg agacgttcgc cgcggtcctg gccgcggagc gcggaccgcg gtgacgagag
     2521 gcggagacgt tcatcagacc gacgcgctga ccctcgcccg gcagatccgc acgaagcagg
     2581 tctcgcccgt cgaggcggtc gaggccacgc tccggcggat cgacgcgctc cagcccaccg
     2641 tcaacgcgtt catcaccgtg accgccgacg aggcgcgcga cgccgcccgc cgggcggagg
     2701 cggcggtgat ggcgggccac cgcctcggcc cgctccacgg cgtgcccttc tcggtcaagg
     2761 acctgctctt caccacgggc gtccgcacca cgatgggctc gctgatcttc gccgaccagg
     2821 tccccgtcgc ggacgcggtg cccgtccggc gtctgcgtga ggccggcgcg atcctgatcg
     2881 gcaagacgac gacgcccgag ttcggccaca agccgctgac cgacagcccg ctcttcggcg
     2941 ccacccgcaa cccgtgggat ctctcgcgca ccgcgggcgg ctcgtcgggc ggcgccgccg
     3001 cggcggttgc caccggccag gggccgctgg ccctcggcac cgacggcggc ggctcggtgc
     3061 gcctgcccgc ctcgtgctgc ggcatcgtcg ggctcaagcc gacgctcggg cgcgtgccgc
     3121 acacgcagca ggccgacctc ttctcctcga cttcctacat cggcccgatg gcgcggagcg
     3181 tggccgagac ggcggcctgc ttcgacgcga tcgtcggatt cgacccgggc gatccgtact
     3241 cgcgcccgga gccgccggac gatccgcgcg acgtcgcggt gcgcggcctg cgcctcggct
     3301 ggctgccgcg ggtgggcaat cggctcgtcg acccggaagt gctcgccgcc tgcgaggccg
     3361 cggtgcggca tctggaaggc cagggcgccc acgtcgagac cctggaggaa gacgtctcgg
     3421 cctacgagcg caccttcctg atcggcctgc aggcgggcct gaccgcgcgc gtcggcgggc
     3481 acatggcgaa gttcggcgac aaggtcgcgc cgagcctgcg cgagtccatc gagcggggcg
     3541 cgcagtggtc ggcggtggac tgggtgaccg cgctcggcca gcggaccgcg atgtaccgcc
     3601 gggtgaacgc gctgctccag cgcttcgatt tcctgctctc gccgacgctg tcccgtcccg
     3661 cgctcgccgt cgaccacgac gcgttcgagc cgatcacgat cgcgggcgag cccgccggct
     3721 ccatccgcgg cgcctggtat ccctatctct ggcccttcaa cctctccggc cacccggcgg
     3781 tctcgctgcc gtgcggctgg tcctccgacg gcctgccgat cgggctccag atcgtcggtc
     3841 cctggtacgg cgaccgccgc gtcctcgcgc tggccggcca cctcgagcgc gagcgtccct
     3901 gcgcgcgccc gatgcctctc taactctctc tatcgctccg gagacacgcc atgccgctct
     3961 cgccgaaggg gttcccggtc ttgctcagct tcgacatcga cgccgagacc atgtggaccg
     4021 ggcgcgaccc caagaacgcc gagcgcccca tcgtgatgag ccagggcgcg tacggctgga
     4081 aggtggggat gccgcgcatc ctcgacctgc tcgcgcgcta cggcctgcgc gtcaccttct
     4141 tcgtgccggg tctcgtcatg gagcagcgcc cggccatggt cgaggccatc ctgaaggccg
     4201 gccacgagat cgcgcatcac tcgtggtcgc acgcctggat cgtcaacctg acgctcgagc
     4261 aggagcgcga ggagatggac cggggcatcg agatcatcaa gcgcatgacg gggcgaaacc
     4321 cggtcggcta ccgctccccg gcggcggagt tcagccccca tacgctgaag atgctcgccg
     4381 agtacaagtt cggctactcc tcgaactact tcgacgacga ctcgccctat ctccaccgga
     4441 tcgacggcaa gctcaccgac atcgtcgagt tcccgttcgc gtgggtgctg gacgacgcgc
     4501 cgttcttcca gtactcgatc acgctgcccg ggcgcaccat gcaggccccg tccgccgtct
     4561 tcgaggcctg gcgccgggag ttcgacgtgc tctaccgcga ggaccggtac ttctgcctcg
     4621 ccatgcatcc ccagatcatc gggcgcccgt cgcgcatcac cctgctggag gagctgatcc
     4681 ggtacatcct cggccacccg cgggtctggc tcgcgcgctg cgacgaggcg gcggacgcgc
     4741 tgcggccggc gctgcgcgcc gaggcgcgcg cgtgaggctc gccggccgcc gggtgctcgt
     4801 gaccggcgcg gcctctggca tcggcctcgc gatcgcgcgg ctgttccgga gcgagggcgc
     4861 ggcggtggcc atgctcgacc gcgacgaggc gggcctggcg aaggcgcagg tcgacggcgc
     4921 cgccaccctg gtctgcgacg tggccgagga acggcaggtg cgcgccgccg tcgcgaaggc
     4981 cgcgactgcg ctgggcggcc tcgacggcgt cgtcaacagc gccggcatcg acctgatgcg
     5041 gcccttcgag cggatgacgt cggaggagtg ggcgcggatc atggcggtgg atctcaccgg
     5101 ccccatgctc gtctgccacg ccgcgctccc ggcgctcaag caggccgggc gcgggacgat
     5161 cgtcaacatc gcctccggcg cggcgctccg gccgctcgag cagcggaccg cctactgcac
     5221 cgcgaaggcg ggcctcgtca tgttcggcaa gaccctcgcg gtggacctgg ccgccgacca
     5281 cattcgggtg aacgcgatct gccccggcat catcgacacg ccgctctttc gctcctcgtg
     5341 ggagggggcg cccgacccgg aggccgagct ggcgcggatc ctcgatcgct acgtgatcaa
     5401 gcggccgggg gagccggagg agatcgcgca ggcggcgctc tatctgacca gcgacgagtc
     5461 gtcgttcgtc accggggcgg cgctggcagt ggacgggggg cggacgtttc actgaggcgc
     5521 gtgcctcagc ctgccccccc cctcaccctg ccccaccccc ca
//
LOCUS       DATMCV010000224         3009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_146861, whole genome shotgun sequence.
ACCESSION   DATMCV010000224 DATMCV010000000
VERSION     DATMCV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146861"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VNU03_05295"
     CDS             complement(<1..466)
                     /locus_tag="VNU03_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73904.1"
                     /translation="MNPLDMSKRQVLKDRRAVLAMRRFATRCTVGVLALLCLGCLAAL
                     RGDREPHGNWPAYGNDAGGGRYSPLSQIDRGNVGRLRVAWTYRTGEADDRSPARQKSA
                     FEATPIMVDRTLFLSTPFNRVIALDPETGAERWVYDPRVDRERTYALVTSRGV"
     gene            complement(694..1407)
                     /locus_tag="VNU03_05300"
     CDS             complement(694..1407)
                     /locus_tag="VNU03_05300"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWP73905.1"
                     /translation="MKLVHQGTILGEDNERMSKSRGNVVNPDDIVREYGADALRLYEM
                     FMGPLEAVKPWQTSQIQGVVRFRDRLHGVCRQPLVEAMDDATRRLLHRTIKKVSTDID
                     AMAFNTAISALMVFVNHLARLSAMPREAVRALVLLVSPFAPHVAEELWQHLGHAASLA
                     YEPWPAWDEALAVDDVIELPVQVNGKVRGRVQLARDASEADARAAALAADGIAARLAG
                     QAVRKFVYVPGQVVNLVVG"
     gene            complement(1404..>3009)
                     /locus_tag="VNU03_05305"
     CDS             complement(1404..>3009)
                     /locus_tag="VNU03_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWP73906.1"
                     /translation="HVGHPEGYTATDILARFRRMNGYHVLHPMGWDAFGLPAEQHAIE
                     TGQHPAETTAHNVATFKRQLKMLGFSYDWSREIDTTDPGYVRWTQWIFLQLFRRGLAY
                     QATVPVNWCPALGTVLANEEVVDGKSERGGHPVYRTPLRQWMLKITAYADRLAEDLAL
                     VDWPEGTLAAQRRWIGGSEGAEIVFGVDGHPDARIAVFTTRPDTLMGATYVVLAPEHE
                     LACTLAAPARREAVVAYVEAAARKSELDRVALVKTKTGASTGAVAIHPITGARLPIWV
                     ADYVLGGYGTGAVMAVPGHDQRDFEFAIAFGLPIVEVVSPDGRLHDALEAAYVDDGVN
                     VRSGEFDGLATPAAKGAVVRDSRRSAAASARSATGCATGSSRVSATGASPSRSTFPSS
                     SLTRPATLGEGPRTRFAMTSRSRSTSPSCRCACPSSPISTRVRTRPARSRASASGASS
                     SATVAGSHARPTPCRNGRARAGTTCGFSTRTTTTSPGRWTRTTRGCPSTSTSAAPSTR
                     CSTSSTPASGTRCSSISVTSGTPSRS"
BASE COUNT          465 a         1049 c         1046 g          449 t
ORIGIN      
        1 agacgccgcg cgaggtcacc agcgcgtagg tccgctcccg atcgacgcgg ggatcgtaga
       61 cccagcgctc ggctccggtc tccggatcga gcgcgatcac gcggttgaag ggcgtgctca
      121 ggaacagcgt gcggtccacc atgatcggcg tggcctcgaa ggccgacttc tggcgcgccg
      181 ggctccgatc gtcggcctcg ccggtccggt aggtccacgc cacccggagg cggccgacat
      241 tcccgcggtc gatctggctc aggggtgagt agcgcccccc gcccgcgtcg ttgccgtagg
      301 ccggccagtt cccgtggggc tcccgatcgc cgcgcagggc ggcaagacag cccaggcaca
      361 gcagcgcgag cacgccgacc gtgcatcggg tggcgaagcg cctcatcgcg aggacagcgc
      421 gtcgatcttt aagcacctgg cgcttcgaca tgtcgagggg gttcatggac cggacctcct
      481 tccttggcag ggtagcggcc cagcgatgcc accgtccaac gcatatctct taccccaccg
      541 atcagccttt gcaatgcccc cgcgcagatc gagggctgga tttgaaccag cgacctttgg
      601 gttatgagag gttcacccgc aatcatccta gttcccgctc tcagcggcac ccaagtcacg
      661 cgatctccat ggcgagccgc catccgagtg tcttcagccg accacgagat tcacgacctg
      721 ccccggcacg tagacgaact tccgcaccgc ctgcccggcc aggcgcgccg cgatcccatc
      781 cgcggccaac gccgccgcgc gcgcgtccgc ctcggaggcg tcgcgggcga gctggacgcg
      841 tccgcgcacc ttgccgttta cctggacggg gagctcgatc acgtcgtcga ccgcgagggc
      901 ctcgtcccac gctggccacg gctcgtacgc gagcgatgcc gcgtggccga gatgctgcca
      961 gagctcctcg gcgacgtgcg gcgcgaacgg cgagacgagc aggaccagcg cccgcaccgc
     1021 ctcgcgcggc atcgcggaga ggcgggcgag gtggttcacg aagaccatga gagctgagat
     1081 ggccgtattg aaggccatcg cgtcgatgtc ggtgctgacc ttcttgatgg tccggtggag
     1141 cagccggcgc gtggcgtcgt ccatcgcctc cacgaggggc tggcgacaga ccccgtggag
     1201 ccggtcgcgg aagcgaacca cgccctggat ctgcgaggtc tgccacggct tcacggcctc
     1261 gagcggcccc atgaacatct cgtagagccg gagcgcgtcg gcgccgtact cccgcacgat
     1321 gtcgtcggga ttcacgacgt tgccccgcga cttcgacatc cgctcgttgt cctcgcccag
     1381 gatcgtcccc tggtgcacga gcttcatgaa cggctcgggg tgccggacgt gaccgagatc
     1441 gaagagcacc ttgtgccaga agcgggcgta gaggaggtgg agcaccgcgt gctcggcgcc
     1501 gccgacgtag aggtcgacgg gcatccacgc gtcgtacgcg tccatcgccc agggcttgtc
     1561 gtcgtcgtcc gggtcgagaa accgcaggta gtaccagcac gagcccgccc attgcggcat
     1621 ggtgttggtc tcgcgtgcga accagcgacc gtcgcgctgg aagaagcgcc agacgccgac
     1681 gcgcgcgagc gggccggccg ggtcctcacc cgggttgaaa tcggcgagct cgggcaggcg
     1741 cagcggcagc tcggactcgt cgaccgcgag cggctggtca tagcgaatcg tgtgcgcggc
     1801 ccctcgccga gggtcgccgg tcgggtcagc gagctcgacg ggaaagtaga tcgggatggg
     1861 ctcgccccag tagcgctgac gcgagaagac ccagtcgcgc agccggtagc tgaccgtgcc
     1921 gaggccgcgg ccgagcgtct cgagtcgcgg acgaccgccc ctttggccgc gggcgtcgcc
     1981 aggccgtcga actcgccgct gcggacgttg acgccgtcgt cgacgtacgc ggcctcgaga
     2041 gcgtcgtgca gtcgcccgtc cgggctcacg acctcgacga tcggaagacc gaaggcgatc
     2101 gcgaactcga agtcgcgctg atcgtgcccg gggacggcca tgacggcgcc ggtcccgtag
     2161 ccgccaagca cgtagtcggc cacccagatc ggcaggcgag ctccggtgat cgggtggatg
     2221 gccaccgcgc cggtcgaagc cccggtcttc gtcttgacga gcgcgacgcg gtccagctcg
     2281 ctcttgcggg cggccgcctc gacgtacgcg acgacggcct cgcgccgagc cggcgccgcc
     2341 agcgtgcacg ccagctcgtg ctcgggcgcg agcacgacgt aggtcgcgcc catgagggta
     2401 tcgggccgcg tcgtgaagac ggcgatgcgt gcgtccgggt gaccgtcgac accgaagacg
     2461 atctcggcgc cctccgaacc gccgatccag cggcgctgcg cggcaagcgt cccctcgggc
     2521 cagtcgacga gcgccaggtc ctcggcgagc cgatcggcat acgcggtgat cttcagcatc
     2581 cattgtcgga gcggcgtgcg atagacggga tggccgccgc gctcgctctt gccatcgacg
     2641 acctcctcgt tggcaagcac ggtgccgagc gcgggacacc agttgacggg caccgtcgcc
     2701 tggtacgcga gcccgcgccg gaacagctgc aggaagatcc actgggtcca gcgcacgtag
     2761 cccggatccg tggtgtcgat ctcccgcgac cagtcgtaac tgaagccgag catcttgagc
     2821 tggcgcttga acgtcgcgac gttgtgcgcc gtcgtctccg cgggatgctg cccggtctcg
     2881 atcgcgtgct gctcggccgg caggccgaag gcgtcccagc ccatcgggtg caggacgtgg
     2941 tagccgttca tccgccggaa ccgggctagg atatcggtcg ccgtgtaccc ctccgggtgg
     3001 ccgacgtgc
//
LOCUS       DATMCV010000225         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_946011, whole genome shotgun sequence.
ACCESSION   DATMCV010000225 DATMCV010000000
VERSION     DATMCV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946011"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VNU03_05310"
     CDS             complement(<1..497)
                     /locus_tag="VNU03_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP73907.1"
                     /translation="MGVLDGKVALVTGAGRLRGIGRATALGLAELGADVAVTGTGRDP
                     ASYPEDEKKAGWRDVESTAEQIRAKGRRAMTWTGDVSQAEPVGRLVDQVVQTFGRLDV
                     LVNNAAYPRGNDRVPLPELDEAVWRKVLDIKLTGAFLLCKRVVPIMVRQKWGRIVNLS
                     SIAGKR"
     gene            complement(515..1756)
                     /locus_tag="VNU03_05315"
     CDS             complement(515..1756)
                     /locus_tag="VNU03_05315"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73908.1"
                     /translation="MSGTRPGVVLTALTGLNGLNYLDRYVAAATLPLILAGLAISDTA
                     GGLLQSAFILTYSLACPIAGWLGDRHPRMRMAAAGVFVWSAATLASGLAPTYAFLLLA
                     RSIIGVGEASYAVVTPSLLSDCYPPERRSRVLGIFYAAIPVGTALGYVIGGLVGEAYG
                     WRAAFFVAGAPGALLAVLLLLLPEPPRGALDPAGAVAATPLGLGASLRALVARRSYLV
                     NTGAQVIYTFAMGGLATWMPTYFVRERGIPLGTAATTFGLILVVAGFAGTLVGGRLSD
                     AFAKRSRSAHFTLSGASLVLSIVFTLPAILAPSPAVFWPAMFATLFLLFMNIGPLNAA
                     MANVLPADLRARGFAVTTMVMHLLGDAASPYLIGLVSDRIGLTAPVLFTGCMLAVSGV
                     VLLLGRATLETDLERLEARRA"
     gene            complement(1782..>2269)
                     /locus_tag="VNU03_05320"
     CDS             complement(1782..>2269)
                     /locus_tag="VNU03_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HWP73909.1"
                     /translation="VTGVEGMPALADAGNVLRPKTSVKLSLRLPPTADADAASRRVKE
                     VLEADPPYGARVTYASGERPGTGWNAPVMADWLRDSVGRASTAQFGAPAMFDGLGGSI
                     PFMAMLGERFPRAQFLVTGVMGPGSNAHGPNEFIDLPTGVKVTACVAQVIADHGASRA
                     R"
BASE COUNT          344 a          837 c          830 g          258 t
ORIGIN      
        1 cgcttgcccg cgatcgagga gaggttgacg atgcgccccc acttctgccg gaccatgatc
       61 gggaccacgc gcttgcagag gaggaacgcg ccggtcagct tgatgtcgag caccttgcgc
      121 cagaccgcct cgtccagctc gggcaagggc acgcgatcgt tgccccgcgg gtacgccgcg
      181 ttgttcacga gcacgtcgag gcgcccgaag gtctggacga cctggtccac caggcgcccc
      241 accggctcgg cctggctcac gtcgccggtc caggtcatgg cgcgacggcc cttcgcgcgg
      301 atctgctcgg cggtgctctc gacgtcgcgc cagcccgcct tcttctcgtc ctccgggtag
      361 ctcgccggat cgcgcccggt cccggtgacc gccacgtccg ccccgagctc ggccaggccc
      421 agcgcggtgg cccgcccgat gccgcggagg cgccccgccc ccgtcaccag cgcgaccttg
      481 ccatccaaga cgcccatgtc gtgtcctttc gcgctcaggc ccgccgggcc tcgagcctct
      541 cgagatcggt ctcgagcgtg gcccgcccga ggagcaggac gacgcccgag accgccagca
      601 tacagccggt gaacagcacc ggcgcggtca gcccgatccg gtcggagacg agcccgatca
      661 ggtagggcga cgccgcgtcg cccaggaggt gcatcaccat cgtggtcacc gcgaagcccc
      721 gcgcgcgcag gtccgcgggc agcacgttgg ccatcgccgc gttgagcggg ccgatgttca
      781 tgaagagcag gaagagcgtc gcgaacatgg cgggccagaa taccgccggc gacggggcca
      841 ggatcgcggg cagcgtgaac acgatcgaga gcaccaggct cgcgccggac agcgtgaagt
      901 gggccgagcg cgagcgcttg gcgaaggcgt cgctcagccg gccgccgacc agggtgcccg
      961 cgaagcccgc gaccaccagg atcaagccga aggtggtggc ggcggtcccc aggggaatgc
     1021 cccgctcgcg cacgaagtag gtcggcatcc acgtcgcgag cccgcccatc gcgaaggtgt
     1081 agatcacctg ggccccggtg ttgaccaggt agctccgccg cgccaccagc gcgcgcagcg
     1141 acgccccgag gccgagcggg gtggccgcga ccgcgcccgc cggatcgagc gcgccgcgtg
     1201 gcggctcggg cagcagaagg agcagcaccg cgagcagcgc gccgggggcg cccgccacga
     1261 agaacgcggc gcgccagccg taggcctcgc cgaccagccc gccgatcacg tagccgagcg
     1321 cggtgcccac cgggatcgcg gcgtagaaga tgccgaggac ccgcgagcgg cgctccggcg
     1381 gatagcagtc ggacagcagc gacggggtga ccaccgcgta gctcgcctcg ccgaccccga
     1441 tgatcgaccg ggcgagcagc aggaaggcgt acgtcggcgc caggccggag gccagcgtcg
     1501 ccgcgctcca gacgaagacg ccggccgcgg ccatgcgcat gcggggatgg cggtcgccga
     1561 gccagcccgc gatcgggcac gccagcgagt aggtcaggat gaaggccgac tggagcagcc
     1621 cgcccgcggt gtccgagatc gcgagcccgg ccaggatgag cggcagcgtc gcggccgcga
     1681 cgtagcgatc gaggtagttg aggccgttga gcccggtgag agcggtgagg acgacgccgg
     1741 gccgggtgcc gctcacgggc cctcggccgg cctcggccgg atcagcgcgc ccggctcgcg
     1801 ccgtggtcgg cgatcacctg cgccacgcag gcggtcacct tcactcccgt cggcagatcg
     1861 atgaactcgt tcggcccgtg cgcgttcgag cccggcccca tcaccccggt gaccaggaac
     1921 tgggcccgcg ggaaccgctc gcccagcatg gccatgaacg ggatcgagcc gccgaggccg
     1981 tcgaacatcg cgggcgcgcc gaactgcgcg gtcgacgcgc ggccgaccga gtcgcggagc
     2041 cagtcggcca tcaccggcgc gttccacccg gtgccgggcc gctcgccgct cgcgtaggtc
     2101 acgcgggcgc cgtagggcgg atcggcctcg agcacttcct tgacccggcg gctagccgcg
     2161 tccgcgtcgg cggtgggcgg caggcgcagc gagagcttca ccgaggtctt cgggcgcagc
     2221 acgttgcccg cgtcggcgag cgcgggcata ccctcgacac cggtgaccg
//
LOCUS       DATMCV010000226         2752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_946040, whole genome shotgun sequence.
ACCESSION   DATMCV010000226 DATMCV010000000
VERSION     DATMCV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946040"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VNU03_05325"
     CDS             <1..168
                     /locus_tag="VNU03_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein"
                     /protein_id="HWP73910.1"
                     /translation="DGVTILLVEQRVAEALERSDHGYVLETGHIVLEGRYETLLADDR
                     VRRSYLGLTEA"
     gene            214..1464
                     /locus_tag="VNU03_05330"
     CDS             214..1464
                     /locus_tag="VNU03_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP73911.1"
                     /translation="MTTPEPIGRRTAIKMGLATAAAAALPTRRARAQAPKEVKIALVV
                     PLSGAWARQGILEQMGAEMAIEDVNKAGGIKALGGAKMKLVLYDTGDTAEKAKNAAQR
                     LVAQEPDVVGGIGCWLSSFTLAATEVSERAELPWLTLSYSDLITGRGYKYVFQSSPTA
                     EAQSTALLPRIVDLATKATNVKPTKVAFVGDNSASSVSFMKPIREHVAKDLGLTIVAD
                     EVYTPPLADATTLVQKIRSGRPDFIVCQSTNVGDDKLLIDKFAERGITPSTVPLIGGG
                     GHWCVPELMKVAGKEHLEGMIVGLANWPGRAVEDVEKRFIAKTGEPWFGHDSIFPYAH
                     VWIFKEALERTGSVDRKKLGEMMHKIDLKDGPAKFFPDGRVKYEENGRRVGAELCIVQ
                     WQKGRPVAVHPESIAASKAIWPKS"
     gene            1543..2487
                     /locus_tag="VNU03_05335"
     CDS             1543..2487
                     /locus_tag="VNU03_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73912.1"
                     /translation="MPSSIGPRRPGYRSIFTTATRRQRAANFEAYWRYTVEHDGEIRE
                     DEKVLSKKQQVLTRFQSNPVRSTHPLPDAERFYRNHVTMRDDPRALDRKTLLLTFLYK
                     FARHEYVGISAAWGASSRITEASSTIAKIGRYHLCEEFSHMRLFHEMFRTFHLDRVEW
                     VPLGKWMSRVYRLFPHVPEALMAPPAFVTELMGLTVYQHVDAILDEILADELDTRDRI
                     RQLLSEIMTDELAHVGQRRNFMGPIGLRAARRFVEPMYRAFFRDIPETRFLFDLARMV
                     QDGKTFDYSTISPGMIERSWVPSYCRASEPRHVGGHLW"
     gene            2502..>2752
                     /locus_tag="VNU03_05340"
     CDS             2502..>2752
                     /locus_tag="VNU03_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HWP73913.1"
                     /translation="MNGIVAYGAYLPYFRLDRKAIGDALGSGGGKGTRVVASYDEDTT
                     SMGVEAARAALRGAPRLAPAAVYFATAEPAYLDKTNATA"
BASE COUNT          475 a          944 c          930 g          403 t
ORIGIN      
        1 gacggggtga cgatcctcct ggtcgagcag cgcgtggccg aggccctcga gcgctcggat
       61 cacggctacg tgctggagac ggggcacatc gtgctcgagg ggcggtacga gaccttgctg
      121 gccgacgatc gggtccgccg ctcgtatctc ggcctgaccg aagcctagat cgattcacca
      181 tcacatcatc accaacgagg gagatgaccg aacatgacga cgcctgagcc gatcggacgc
      241 cggaccgcca tcaagatggg cctcgccacc gctgccgcgg cggcgctccc gacccgccgc
      301 gcgcgcgcgc aagcacccaa ggaagtaaaa atcgccctgg tcgtcccgct atccggggcc
      361 tgggcgcgcc agggcatcct cgagcagatg ggggccgaga tggccatcga ggacgtgaac
      421 aaggcgggcg gcatcaaggc cctcggcggc gccaagatga agctggtcct ctacgacacc
      481 ggcgacaccg cggagaaggc caagaacgcg gcgcagcgcc tggtggcgca ggagcccgac
      541 gtggtcggcg gcatcggctg ctggctgtcg agcttcacgc tcgcggccac cgaggtgtcg
      601 gagcgcgcgg agctgccgtg gctcaccctg tcctactccg acctcatcac cggccgcggc
      661 tacaagtacg tcttccagtc gtcgccgacc gcggaggcgc agtccaccgc gctgctgccg
      721 cgcatcgtgg acctcgcgac caaggccacc aacgtgaagc cgaccaaggt agccttcgtg
      781 ggcgacaact cggcctcgtc ggtgagcttc atgaagccca tccgggagca cgtggccaag
      841 gacctgggcc tcacgatcgt ggccgacgag gtctacactc cgccgctcgc ggacgcgacc
      901 acgctggtgc agaagatccg ctccgggcgg cctgacttca tcgtgtgcca gtccacgaac
      961 gtgggcgacg acaagctcct gatcgacaag ttcgcggagc gggggatcac gccctccacc
     1021 gtcccgctga tcggcggcgg cggccactgg tgcgtgccgg agctgatgaa ggtggcgggc
     1081 aaggagcacc tggagggcat gatcgtgggg ctcgccaact ggccgggccg ggcggtggag
     1141 gacgtcgaga agcgcttcat cgccaagacc ggcgagccgt ggttcggcca cgactcgatc
     1201 ttcccctacg cgcacgtctg gatcttcaag gaggcgctgg agcggaccgg ctccgtcgac
     1261 cgcaagaagc tgggcgagat gatgcacaag atcgacctca aggacggtcc ggccaagttc
     1321 ttcccggacg gccgcgtgaa gtacgaggag aacggccgcc gggtgggcgc cgagctctgc
     1381 atcgtgcagt ggcagaaggg ccggccggtg gcggtgcatc cggagagcat cgcggcctcg
     1441 aaggcgatct ggccgaagtc gtaggggtcg ggtcaggccg gccggtcgac gggaggcccc
     1501 gtcgaccggc cggcactagc gcggtgtgag tacgatgagg ccatgcccag ctccatcggg
     1561 ccccgacgtc ctggctatcg gtcgatcttc accacggcca cgcggcgcca gcgcgccgcg
     1621 aacttcgaag cctactggcg ctacaccgtg gagcacgacg gagagatccg ggaggacgag
     1681 aaggtcctca gcaagaaaca gcaagtcctg acccgctttc agtcgaaccc ggtccggtcc
     1741 acgcacccgc tcccggacgc cgagcggttc taccgcaacc acgtcacgat gcgggacgat
     1801 ccgcgggctc tcgaccgcaa gacgctgctc ctgaccttcc tctacaagtt cgcccggcac
     1861 gagtacgtcg gcatctcggc cgcctggggg gcgagctcgc gaatcaccga ggccagctcg
     1921 acgatcgcca agatcggccg ctaccatctc tgcgaagagt tcagccacat gcgcctcttc
     1981 cacgagatgt tccggacctt ccatcttgac cgggtcgagt gggtgccgct cgggaagtgg
     2041 atgagccgcg tgtaccgtct cttcccgcac gtgccggaag cgctcatggc cccgcccgcc
     2101 ttcgtgaccg agctgatggg gctgaccgtc taccagcacg tcgacgccat cctggacgag
     2161 attctcgccg acgagctgga tactcgtgac cggatccggc agctgctgag cgagatcatg
     2221 accgacgagc tggcccacgt cggccagcgc cgcaacttca tggggccgat cgggctccgt
     2281 gcggcccgcc ggttcgtgga gccgatgtac cgggccttct tccgcgacat ccccgaaacg
     2341 agattcctgt tcgacctcgc gcgcatggtc caggacggga agacattcga ctacagcacg
     2401 atctcgcccg gcatgatcga gcggagctgg gttccctcgt attgccgggc gtccgagccc
     2461 aggcacgtgg gcggccacct gtggtagctt cgaccccacc catgaacggc atcgtcgcct
     2521 acggcgccta tctcccgtac ttccggctcg accggaaggc gatcggcgac gctctcggca
     2581 gcgggggcgg gaaggggacc cgggtcgtgg cctcctacga cgaggacacg acctccatgg
     2641 gcgtggaggc ggcgcgcgcg gccctgcgcg gcgccccgcg gcttgcgccc gcggcggtgt
     2701 acttcgcgac cgccgagccc gcgtatctcg acaagaccaa cgccaccgcg at
//
LOCUS       DATMCV010000227         4024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_809147, whole genome shotgun sequence.
ACCESSION   DATMCV010000227 DATMCV010000000
VERSION     DATMCV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809147"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VNU03_05345"
     CDS             <1..631
                     /locus_tag="VNU03_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP73914.1"
                     /translation="VVRFHNDVVAGKWRVGGFDDQRVYELSRKTLGIVGLGNIGKKVA
                     RRAAAFDMAVQYYDIARLTEAEEDALGVRFVLLPELLRTSDVVSLHVPLDDSTRHLLG
                     AREFAQMKPGAILINTCRGPVVDEAALHAALKSGQVAGAGLDVLVEEPPATNHPLFSL
                     PNVTLTPHSAGPTWENWTARFRNGFDNIQRVAAGRAPKWVIPELQDPQP"
     gene            628..1590
                     /locus_tag="VNU03_05350"
     CDS             628..1590
                     /locus_tag="VNU03_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HWP73915.1"
                     /translation="MKILFAPNLIVSGLSEADRAGILEAAGPGARIVEAKDKASQRRE
                     LPDTDVIFGRAHPELFSLATRLVYYHSIGAGVDSILTPELINSDVPLASEKGEVGIHL
                     AEHAFALLLALTRGLHTAIRTPDYELREPIRVHQRELYEQTMGIVGFGGTGRAVARRA
                     VGFGMRVLGVDVERVDPEPGVEAIWPADRLADLLAASDVVVIGLPLTKATHHLFTRER
                     FLQMKRTAILINVTRGEIVRGEDVLGALTEGLIWGVGLDVTDPEPLPKSHPLWTHPRA
                     IVTPHTAGGSPRRAGRVIATFQENLRRMRDGRPLLALIDKQKGY"
     gene            complement(1620..2873)
                     /gene="glgC"
                     /locus_tag="VNU03_05355"
     CDS             complement(1620..2873)
                     /gene="glgC"
                     /locus_tag="VNU03_05355"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWP73916.1"
                     /translation="MRLRILGIVLAGGKGTRLYPLTKERAKPAVPFGGKYRIIDFVLS
                     NLINSGIHSIYVLTQFKSQSLLQHLADGWQFGGLLKDHFIIPVPAQMRSEGETWYRGT
                     ADAIYQNANLIEQSDPHLVAIFSADHIYRMNISSMIAFHEQKRAEITVAAIPVERRFA
                     PEFGVIETARDGHIIGFHEKRADAPTMPGDPDRVYASMGNYIFSTRTLLRELHADAAR
                     EGSSHDFGRDVLPRLVNRLVMFAYDFQTNEIPGEAVDASVYWRDVGTLDAYYEAHMDL
                     RAVSPALNLYNREWPLRTASYADPPAKFTFDDEGRRGQAIDSVVSGGCILSGGMVRNS
                     VLGRHVRVHTGAIVEDSVILDNCDVGRRARIRRAILDKNVRIAEGTTIGYDLEEDKRR
                     HHVTESGIVVVEGLRSSVDISAISV"
     gene            3071..3388
                     /locus_tag="VNU03_05360"
     CDS             3071..3388
                     /locus_tag="VNU03_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWP73917.1"
                     /translation="MSQKLYVGGIAFSTTSEGLRAHFAQSGTVVSASIVTDQFSGQSR
                     GFGFVEMSTAEEAQQAVSQLNDRALDGRSLKVEVAKPKGDKPRGGGGGFGGGRSSGGG
                     GWR"
     gene            complement(3393..>4024)
                     /locus_tag="VNU03_05365"
     CDS             complement(3393..>4024)
                     /locus_tag="VNU03_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HWP73918.1"
                     /translation="GKNYDTWEVPYLPIDPHHVGRSYEAVIRVNSQSGKGGVAYIMKF
                     EHGLDLPRRLQIEFSSVIQKMVEQSGTEITADQIWAAFERTYLTRQEPLALAHHPTVT
                     TAGESGETTIEAPLRVSGTERLAKGVGNGPIAAFVDAVRRDAGIAVAVLDYHEHAIGG
                     GADASAVAYVEVRVGDGRPRWGVGIHGNIVTASLRAVVSAVNREAVTGM"
BASE COUNT          591 a         1428 c         1395 g          610 t
ORIGIN      
        1 ggtggtccgc tttcacaacg acgtggtggc cggcaaatgg cgggtcggcg gcttcgacga
       61 ccagagggtc tacgagctgt cgcgcaagac cctcggcatc gtgggcctcg gcaacatcgg
      121 caagaaggtc gcgcggcgcg cggccgcgtt cgacatggcg gtgcagtact acgacatcgc
      181 ccgcctcacc gaagcggagg aggacgccct cggggtccgc ttcgtgctgc ttcccgagct
      241 gctgcgcacc tccgacgtgg tgagcctgca cgtgccgctc gacgactcca cccgccacct
      301 gctgggggcg cgcgagttcg cccagatgaa gccgggcgcg atcctgatca acacctgccg
      361 gggcccggtg gtggacgagg ccgcgctcca cgccgcgctc aagagcggcc aggtcgcggg
      421 ggcggggctc gacgtcctgg tcgaggagcc gcccgccacc aaccacccgc tcttctcgct
      481 gcccaacgtg accctgaccc cgcactcggc ggggcccacc tgggagaact ggaccgcgcg
      541 gttccgcaac ggcttcgaca acatccagcg ggtggccgcc ggccgcgccc ccaagtgggt
      601 gatccccgag ctgcaggacc cgcagccgtg aagatcctct tcgcgccgaa cctcatcgtc
      661 tccggcctca gcgaggccga ccgcgccggc atcctcgagg cggccggacc cggcgcgcgc
      721 atcgtggaag cgaaggacaa ggcgagccag cgccgcgagc tgcccgacac cgacgtgatc
      781 ttcggccgcg cgcaccccga gctcttctcg ctcgcgaccc ggctcgtgta ctaccactcg
      841 atcggggcgg gggtggacag catcctcacc ccggagctga tcaacagcga cgtgccgctc
      901 gccagcgaga agggcgaggt gggcattcac ctggccgagc acgccttcgc gctgctgctc
      961 gcgctgaccc gcgggctcca caccgcgatc cgcacccccg actacgagct gcgcgagccg
     1021 atccgcgtgc accagcgcga gctctacgag cagaccatgg gcatcgtggg cttcgggggc
     1081 accgggcgcg cggtggcccg gcgcgcggtg ggcttcggca tgcgggtgct cggggtagac
     1141 gtcgagcgcg tcgatcccga gcccggggtg gaggcgatct ggcccgccga ccggctcgcg
     1201 gatctcctgg cggcctccga cgtggtggtg atcggcctgc cgctcaccaa ggccacccac
     1261 cacctgttca cccgcgagcg cttcctccag atgaagcgca ccgcgatcct gatcaacgtc
     1321 acgcgcgggg agatcgtccg gggcgaggac gtgctcggcg cgctcaccga ggggctcatc
     1381 tggggcgtcg gtctcgacgt caccgacccg gagccgctcc cgaagagcca cccgctctgg
     1441 acgcacccgc gggccatcgt caccccgcac accgcggggg gctcgccccg ccgggcgggc
     1501 cgggtgatcg cgacgttcca ggaaaacctg cgacggatgc gagacggccg gccgctgctc
     1561 gccctgatcg acaagcagaa gggctactag cggcagcggc ggccgagggc cgcggcggct
     1621 cagaccgaga tcgcggagat gtccaccgag gagcgcagcc cctcgaccac cacgatgccc
     1681 gactcggtca cgtggtggcg ccgcttgtcc tcctcgaggt cgtagccgat cgtcgtcccc
     1741 tcggcgatgc gcacgttctt gtcgaggatg gcgcggcgga tccgggcccg gcggccgacg
     1801 tcgcagttgt cgaggatcac cgagtcctcg acgatcgcgc cggtgtgcac ccgcacgtgc
     1861 cggccgagca ccgagttgcg gaccatgccc ccggagagga tgcagccccc cgagaccacc
     1921 gagtcgatgg cctggccgcg gcggccctcg tcgtcgaagg tgaacttggc gggcggatcc
     1981 gcgtagctcg cggtccgcag cggccactcc cggttgtaca ggttgagcgc ggggctcacc
     2041 gcccggaggt ccatgtgggc ctcgtagtag gcgtcgaggg tccccacgtc gcgccagtag
     2101 acggaggcgt ccaccgcctc gcccgggatc tcgttggtct ggaagtcgta ggcgaacatg
     2161 acgagccggt tcaccagccg cggcagcacg tcgcgcccga agtcgtggct gctgccctca
     2221 cgggccgcgt ccgcgtgcag ctcgcgcagc agggtccggg tcgagaagat gtagttgccc
     2281 atcgaggcgt agacccggtc ggggtcgccc ggcatggtcg gcgcgtccgc ccgcttctcg
     2341 tggaagccga tgatgtgacc gtcgcgcgcg gtctcgatca ccccgaactc gggcgcgaag
     2401 cggcgctcca ccgggatcgc ggccaccgtg atctcggcgc gcttctgctc gtggaacgcg
     2461 atcatgctcg agatgttcat ccggtagatg tgatcggcgc tgaagatggc gaccaggtgc
     2521 gggtccgact gctcgatcag gttggcgttc tggtagatcg cgtccgcggt gccccggtac
     2581 cacgtctcgc cctcggaccg catctgggcc ggcaccggga tgatgaagtg gtccttgagc
     2641 aggccgccga actgccagcc gtccgctaag tgctgcagca gggactggct cttgaactgg
     2701 gtgagcacgt agatcgagtg gatgccggag ttgatcaggt tgctgaggac gaagtcgatg
     2761 atccggtact tgcccccgaa gggcaccgcc ggcttggcgc gctccttggt caggggatag
     2821 aggcgagtgc ccttcccacc ggccagcacg attccgagga tccgcagcct catggcatcg
     2881 atgctcgccg gtagcctatc acgcaagggg gcgaataccc tgccgggggc gtgccccgcc
     2941 ctgtatactg gctggcgtct ggtacaggta ccggctcacc ttcgagtccc cctcgtcagg
     3001 cggcgtttcg agcccttccg cgcatccgag acgctcaaac agggcggaag cacattcagg
     3061 aggggcaacc atgtcccaga agctctatgt cggcggcatc gcgttcagca ccaccagtga
     3121 gggccttcgc gcgcacttcg cccagtccgg caccgtggtc tcggccagca tcgtgaccga
     3181 ccagttctcc ggccagtcgc gcggcttcgg cttcgtcgag atgtccaccg ccgaggaggc
     3241 gcagcaggcg gtctcccagc tcaacgatcg cgccctcgac ggccgctcgc tgaaggtgga
     3301 ggtcgccaag cccaagggtg acaagccccg tggcggtggc ggcggcttcg gcggcggtcg
     3361 cagcagcggc ggcggcggct ggcgctaacc gctcacatac ccgtcaccgc ctcgcggttg
     3421 accgcgctga ccaccgcgcg aagcgaggcg gtgacgatgt tgccgtggat ccccactccc
     3481 caccgcgggc ggccgtctcc cacccgcacc tcgacgtagg ccaccgcgga ggcgtcggcg
     3541 cccccgccga tcgcgtgctc gtggtagtcg agcaccgcca ctgcgatgcc cgcatcccgc
     3601 ctcaccgcgt ccacgaaggc cgcgatgggg ccgttaccca cgcctttggc gaggcgctcc
     3661 gtgcccgaga cccgtagcgg cgcctcgatg gtggtctcgc cggactctcc cgcggtggtc
     3721 acggtcgggt ggtgcgccag ggcgagcggc tcctggcggg tgaggtaggt ccgctcgaac
     3781 gcggcccaga tctgatccgc ggtgatctcg gtgccgctct gctcgaccat cttctggatg
     3841 accgaggaga actcgatctg cagccgccgc ggcaggtcga ggccgtgctc gaacttcatg
     3901 atgtaggcga ccccgccctt gccggactgg ctgttgaccc ggatcaccgc ttcgtagctc
     3961 cggcccacgt ggtgcgggtc gatgggcagg tacgggacct cccaggtgtc gtagttcttg
     4021 ccga
//
LOCUS       DATMCV010000228         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_878307, whole genome shotgun sequence.
ACCESSION   DATMCV010000228 DATMCV010000000
VERSION     DATMCV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878307"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="VNU03_05370"
     CDS             <1..1960
                     /locus_tag="VNU03_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 51"
                     /protein_id="HWP73919.1"
                     /translation="LRDAALGERLELRRGGAAAPVAFVERKGANLVRARLTSMLSVPA
                     LYDLDRLDLTARSTLDAPAQDAVARLLRSLREPATLERLGLVGEKLLGGGDPTRVLYS
                     VTLYERVQGQNRVRIQTDNLDQPLDINGGARIDLGSTAKLRTLISYLEAIAALHDRYA
                     RLEPAALAKTSVHPRDRLGEWAVQYLTSARGTDRTLQAMLEASLDRRYSASPGEAFFT
                     GGGLHTFGNFDREDDAKVLTIREGFQNSVNLVFIRLMRDVVTHHLYGSSGSLALVLEN
                     PGDPRRDRYLTRFADREGSEFVRRFYRKYHGLPVEEALELVLSAARPTPQGLAIALRA
                     VYPEASPEVFRDLLASRVPGRALSDKAVAELYEQAAPEAMPLVDRGYVAGVHPLELWL
                     AGYLRRHPGAGMGQVVRASAAERQAVYSWLFFTRHRNAQDHRIASLLELEAFLDLHRS
                     WERLGYPFASLTPSLATAIGSSGDRPAALAELMGIILNNGVRQPTVLIDGLDFAAGTP
                     YETRMGRAEIPPSQVLAPEVAAVVRGALGQVVDGGTARGLKPALTGPGGTHTVGGKTG
                     TGDHRYFVYGKNGSVVESRVVSRAATFVFMLDDRYFGTVTAYVPGPEAARFHFTSALP
                     VRIVGLLMPALDRLAASPAPDRLRTAAR"
     gene            complement(1984..>2336)
                     /locus_tag="VNU03_05375"
     CDS             complement(1984..>2336)
                     /locus_tag="VNU03_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HWP73920.1"
                     /translation="TADGRALSVDRVVAVMEGQAALPRKNGELVFEEPWQGRVFGMAV
                     ALHERGRFEWEEFRRALIAQIAAAEARPGPFVYYEIWLRTFEELLAGKGLVTGAELDE
                     TTYQFEFGERDDVF"
BASE COUNT          301 a          913 c          810 g          312 t
ORIGIN      
        1 cctgcgcgac gcggccctcg gcgagcggct cgagctgcgg cgcgggggcg ccgccgcgcc
       61 ggtggcgttc gtggagcgca agggcgccaa cctggtccgg gcccggctca cctcgatgct
      121 gagcgtgccc gccctctacg atctcgaccg cctcgatctc accgcgcgca gcaccctcga
      181 cgcgcccgcc caggacgcgg tggcgcgcct tctgcgctcg ctgcgggagc cggccaccct
      241 cgagcgcctc gggctcgtgg gcgagaagct cctgggcggc ggcgatccga cccgcgtgct
      301 ctacagcgtc acgctctacg agcgcgtcca ggggcagaac cgggtccgca tccagaccga
      361 caatctggac cagccgctcg acatcaacgg cggggcgcgg atcgacctcg gctcgacggc
      421 caagctccgc accctgatct cgtacctcga ggcgatcgcc gcgctccacg accggtacgc
      481 gcggctcgag ccggccgcgc tggccaagac gtcggtgcac ccgcgggatc ggctcgggga
      541 atgggcggtg cagtacctca cgagcgcgcg gggcaccgac cgcacgctcc aggccatgct
      601 cgaggcctcg ctcgatcggc gctactcggc gagcccgggg gaggccttct tcaccggcgg
      661 cggtctccac accttcggca acttcgaccg ggaggacgac gcgaaggtcc tcacgatccg
      721 cgagggcttc cagaactcgg tgaacctcgt cttcatccgc ctgatgcgcg acgtggtcac
      781 ccatcacctc tacggctcct cgggctccct ggccctcgtg ctggagaacc cgggcgaccc
      841 gcgccgcgac cgctacctga cccgcttcgc cgaccgcgag gggagcgagt tcgtccggcg
      901 cttctaccgc aagtaccacg gcctgccggt ggaggaggcg ctcgagctgg tcctgagcgc
      961 ggcccgcccc accccgcagg gcctcgccat cgcgctgcgc gcggtctacc cggaagcgag
     1021 cccggaggtc ttccgcgacc tgctcgcctc ccgcgtgccg ggccgcgcgc tctccgacaa
     1081 ggcggtggcc gagctctacg agcaggccgc gcccgaggcg atgcccctcg tcgaccgcgg
     1141 ctacgtggcg ggcgtgcatc cgctcgagct gtggctggcc ggctatctgc ggcggcatcc
     1201 cggcgcgggc atgggccagg tggtgcgcgc gagcgcggcc gagcggcagg cggtctacag
     1261 ctggctcttc ttcacccgtc accgcaacgc gcaggatcat cgcatcgcga gcctgctcga
     1321 gctggaagcg ttcctcgacc tgcaccggag ctgggagcgg ctcggctacc cgttcgcctc
     1381 gctgaccccc tcgctggcca ccgcgatcgg cagctccggc gaccggcccg ccgcgctcgc
     1441 cgagctgatg ggcatcatcc tgaacaacgg ggtgcgccag cccaccgtgc tgatcgacgg
     1501 cctcgacttc gcggcgggca ccccgtacga gacgcgcatg ggtcgcgccg agatcccgcc
     1561 gagccaggtg ctcgcgcccg aggtggccgc ggtggtccgc ggggccctcg gccaggtggt
     1621 ggacggcggc accgcccgcg ggctcaagcc cgcgctgacc gggccgggcg gcacgcacac
     1681 ggtgggcggc aagaccggca ccggcgacca ccgctacttc gtctacggca agaacggctc
     1741 ggtggtggag tcgcgggtgg tgagccgcgc ggccaccttc gtgttcatgc tcgacgaccg
     1801 ctacttcggc accgtgaccg cctacgtgcc gggccccgag gcggcgcgct tccacttcac
     1861 gagcgcgctg ccggtgcgca tcgtgggcct gctcatgccc gcgctcgacc ggctcgccgc
     1921 ctcgcccgcg cccgaccgcc tccgcaccgc cgcccgctga gggcgccgcc gcgccgaggc
     1981 gggctagaag acgtcgtccc gctcgccgaa ctcgaactgg taggtggtct cgtcgagctc
     2041 cgcgccggtg acgaggccct tgccggccag cagctcctcg aacgtccgga gccagatctc
     2101 gtagtagacg aacgggcccg ggcgcgcctc cgcggccgcg atctgggcga tgagggctcg
     2161 gcggaactcc tcccactcga accgcccgcg ctcgtgcagg gccaccgcca tgccgaagac
     2221 gcggccctgc cacggctcct cgaagacgag ctcgccgttc ttgcgcggca gggcggcctg
     2281 gccctccatc accgccacca cccggtccac cgagagggcc cgcccgtcgg cggtga
//
LOCUS       DATMCV010000229         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_545090, whole genome shotgun sequence.
ACCESSION   DATMCV010000229 DATMCV010000000
VERSION     DATMCV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545090"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VNU03_05380"
     CDS             complement(<1..1389)
                     /locus_tag="VNU03_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73921.1"
                     /translation="MTLAAFVPALRGQFLDWDDVVNFVENPYYRGLGAAQLRWMLTST
                     LMGHYIPLTWMSFGLNYVLGGMNPWGYHLVNLLLHAANAVLVYLVARRLLAAASGGGA
                     QDGRTDPAVAWASAFSALLFGIHPLRVESVAWITERRDVLCGLFFLLAVLAYLIAVER
                     GDRPRSAWRALSLGAFAAALLSKAAAMPLPVVLLLLDVYPLRRLHRGWRRLLTEKAPY
                     VALAGAAALAALIALRRGVAVTSYETYGVLARLGMVAYSTLFYPIALLWPMRLSPMYE
                     LPHRVSLGAWPFYAALLGVIAVSAILVLVRRRWPAGLAAWIYSALMILPVSGVVHSGS
                     QLVNDRYSYLSGIGFALVGGTILRAGLGLRERGRLSTSTSGALAGAAILILLVLGGTT
                     WVQTYAWRDPETLWRWAVDMDPTCSLCHGNLGAAIGSGPTGKARLDEAEEHLRRAIAL
                     RPDSPIPYFNLGN"
     gene            complement(1434..>2212)
                     /locus_tag="VNU03_05385"
     CDS             complement(1434..>2212)
                     /locus_tag="VNU03_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP73922.1"
                     /translation="SVWAFSALMLAPASAAVRLGVDLAPDRYSYLSGLGFTMLAGGAA
                     LEAIRLARRHPLARPVRWAGIFATGVVLGGFGLTSWSFAEVWRDSEPLWRWAVELDPS
                     CSVCHAKLGESALGGVGGAARAVEAEGLFRRAIALRPDLPDAYFNLGTALSVQGRYRE
                     AEPPLRTYMELVPYTPAGPERLGLVYLVQQRYDTALPLLRAAFIRSPDAPGYRASLVQ
                     ALQGRAAELRAHGRGVEADELLAEARVLGPDVPAGSSTRP"
BASE COUNT          396 a          744 c          820 g          252 t
ORIGIN      
        1 gttgccgagg ttgaagtagg ggatcgggct gtctggacgg agagcgatgg ctcggcgcag
       61 gtgctcctcc gcttcgtcga ggcgggcctt tccggtcggg ccgctgccga tggccgcccc
      121 gagattgccg tggcacagcg agcaagtcgg atccatgtcc accgcccatc gccagagcgt
      181 ctccggatcc cgccaggcgt aggtctggac ccacgtggtc ccgcccagca ccaggaggat
      241 caggatcgcg gccccggcca gggcaccgga ggtgcttgtg gatagccggc cccgctcccg
      301 tagcccgagc cccgcccgga ggatggtgcc gcccacgagg gcgaacccga tcccagagag
      361 atagctgtag cggtcgttca cgagctggct gccggagtgc accaccccgc tgaccggcaa
      421 gatcatcagg gccgagtaga tccacgccgc gaggccggcc ggccagcgtc ggcgaacgag
      481 cacgaggatg gcggagaccg cgatgacgcc gagcagcgcg gcgtagaacg gccacgcgcc
      541 caggctgacc cggtgcggca gctcgtacat gggagagagg cgcatcggcc acaggagcgc
      601 gatcggatag aagagagtgc tgtaggcgac catgccgagg cgtgcgagca ctccgtaggt
      661 ctcgtacgag gtgacggcca ctccccgccg gagagcgatc agcgcggcga gggcggccgc
      721 gcccgccagg gccacgtacg gcgctttctc ggtgaggagg cgtctccagc cgcggtggag
      781 gcgtcgcaga gggtagacgt cgagcaggag gaggacgacg gggaggggca tcgcggccgc
      841 cttggacagg agcgcggcgg cgaacgcccc gagcgagagc gcccgccagg ccgagcgagg
      901 tcggtcgcca cgctccacgg caatcagata tgccaggacc gcgaggagaa agaagaggcc
      961 gcagagcaca tcgcgccgct cggtgatcca ggccaccgat tcgacccgga gcggatggat
     1021 cccgaagaga agggcgctga acgcgcttgc ccaggcgaca gccggatccg ttcgcccgtc
     1081 ctgcgcgccg ccgccgctcg cggccgccag caaccggcgg gcaaccagat acaccaggac
     1141 cgcgttcgcg gcgtgaagga gcaggttgac gaggtgatag ccccatgggt tcatcccgcc
     1201 gagcacgtag ttgaggccga aggacatcca cgtcagcggg atgtagtgcc ccatgagcgt
     1261 ggacgtgagc atccagcgga gctgggctgc cccaaggccc cggtagtagg ggttctccac
     1321 gaagttcacg acgtcgtccc agtccaggaa ctgaccccgc agcgccggga cgaaagccgc
     1381 gagcgtcacg cagaagatta gagccgggag cagtcgccgg aggggcgggc ctgtcacggg
     1441 cgggtggaag agccggcggg gacgtccggg ccgagaactc gcgcctcggc caggagctcg
     1501 tcggcttcga cgcctcgccc atgagcccgg agctccgccg cgcgtccctg gagggcctgc
     1561 acgagggagg ctcgatagcc gggagcgtcg gggctccgga tgaaggcggc gcgcagcagg
     1621 gggagggcgg tgtcgtagcg ttgctggacc aggtacacga ggccgagccg ttcgggacca
     1681 gccggggtgt acggaaccag ctccatgtag gtgcgcagcg gcggctctgc ctcgcggtag
     1741 cgaccctgga ccgacagcgc agtcccgagg ttgaagtacg catcgggcag atccgggcgc
     1801 agggcgatcg cacgacggaa gagcccctcg gcctcgaccg cccgtgccgc gcctccgacc
     1861 ccgccgagcg cgctctcgcc gagcttggcg tgacagacgc tacagctcgg atcgagctcc
     1921 accgcccagc gccacaacgg ctccgagtcc cgccagacct cggcaaagct ccaggaggtg
     1981 agaccgaagc cgcccaggac gacgcccgtc gcgaagatcc cggcccacct gactggccgc
     2041 gccaaggggt gacggcgggc gagccggatc gcctcgagcg cggcaccgcc cgccagcatc
     2101 gtgaagccca acccggacag atagctgtac cggtcggggg cgaggtccac gccgagccgc
     2161 accgccgcgc tcgcgggagc gagcatcagc gcggagaagg cccagacgct ca
//
LOCUS       DATMCV010000230         3972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_809267, whole genome shotgun sequence.
ACCESSION   DATMCV010000230 DATMCV010000000
VERSION     DATMCV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809267"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VNU03_05390"
     CDS             complement(<1..449)
                     /locus_tag="VNU03_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP73923.1"
                     /translation="METRVDEIAERIYRLSTFLPAVGPKGLTFNQFLVDADEPLLFHC
                     GQRALYPAVSTAAARVMDLRRLRFITFSHIEADECGALAEWLDAAPSATVAHGAIGCA
                     IWLNDQSPRPPRALANDEVLDLGGRRVRRLDTPHVPHCWDAGLLYEET"
     gene            521..1585
                     /locus_tag="VNU03_05395"
     CDS             521..1585
                     /locus_tag="VNU03_05395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP73924.1"
                     /translation="MRFGTFFFFQAAPGLSHAEVVHRELEQMEWTEELGFDEIWLTEH
                     HFIDYGLAVDPASLAATAAARTRRVRIGLAAAILPFHHPLRLAEQMALIDIISKGRLD
                     VGVGRGNRPAEFRGYHVPQQENRERFDEAVDIILKAWTQERFTHEGRFYTIGEARVIP
                     KPWQRPHPPIYQVCGSDDGIESSAARGWPMLNSLLTGPVDQLIKRRDSYLTALRKHGR
                     TEPEITTLMKDWGVSRQIYVADTDAQALAEAKDAEMWYQDSLRRFQVPERIEDAHPSL
                     QPGFRAAAERFKKVTWEGLVAETVAFGSPDTVARHIQTMRDMGVGHVLCWMNFGGLPQ
                     DRIRRSMELFAREVMPHFRG"
     gene            1615..2961
                     /gene="thrC"
                     /locus_tag="VNU03_05400"
     CDS             1615..2961
                     /gene="thrC"
                     /locus_tag="VNU03_05400"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWP73925.1"
                     /translation="MAYNAWFQCINGCPGEFDLREVIYRCPTCGDLLEVQHDMDALRS
                     RSAAAWIQLFDDRYRRNIYPYGSGVWGKKEWVVPFIDNQNIVSMYEGNSNLLWADRYG
                     KQLRVEDLWIKQCGNSHSGSFKDLGMTVLVSVVKEMMAGGSPIDAVACASTGDTSAAL
                     GAYCAAAGIPSVVLLPKDKISIAQLVQPLANGALVLSLDTDFDGCMAIVQEITREKRI
                     YLANSMNSLRIEGQKTVAIEIVQQFDWEVPDWIVIPGGNLGNVHALGQGFLMMQELGL
                     VNRLPRICLAQAERANPLYRSFQNDFKTFEPITAQPTLATAIQIGNPVSVKRAIRTLQ
                     RFNGVVEQATEQELADEAARADRTGMFNCPHTGVALAALRKLVDRKVIRSGERVVVLS
                     TANGLKFADQKAAYHMGTLEGIKPSYRNKPVELPADAKRVARTIARHVEKARALVS"
     gene            2961..>3972
                     /locus_tag="VNU03_05405"
     CDS             2961..>3972
                     /locus_tag="VNU03_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWP73926.1"
                     /translation="MADRPEPGPVDPSRKPVGPSTLSVHGGEPRPKPAHALATPIVQT
                     ATYTFSSTQELKDHFDGKIERVEYGRYGNPTQRIAEAKLAALEGAGDCLLFGSGMGAM
                     TTALFAMLSRGTHVVVTDDSYRRTRQFLNQTLSRYGIEVSTVPAGDYEALEDAIKPTT
                     RVLVSESPTNPYNRILDLERFATIGRSHRVKTVIDATFATPYNQRPLEWGVDVVVHSA
                     TKYLGGHNDLLAGAVLGSAELVGAVRGLQGITGAVLDPFAAYLLVRGLKTFALRMERQ
                     NANAQAVAEFLAGHPRIAAVHYAGLSTHPEHAVARKQMRGFGGVVSFEVAGDLDAATR
                     VVD"
BASE COUNT          632 a         1403 c         1377 g          560 t
ORIGIN      
        1 gtctcctcgt acagcaggcc cgcgtcccag cagtgcggca cgtgcggggt gtcgaggcgg
       61 cgcacgcggc ggccgcccag gtcgaggacc tcgtcgttgg cgagggcgcg cgggggccgc
      121 ggcgactgat cgttgagcca gatcgcgcag ccgatggccc cgtgggcgac ggtggccgac
      181 ggcgcggcgt cgagccattc cgcgagggcg ccgcactcgt cggcctcgat gtggctgaag
      241 gtgatgaagc gcagccggcg caggtccatc acgcgggccg ccgcggtcga gacggccggg
      301 tagagggcgc gctggccgca gtggaagagc aggggctcgt ccgcgtcgac caggaactgg
      361 ttgaaggtca agcccttcgg acccaccgcg ggtagaaagg tcgagagccg gtagatccgc
      421 tcggcgatct cgtcgacacg tgtctccatc gagttttcct ccggctcgca tactgctcgg
      481 ggctcgcgga ggtgtcaagg agagctataa tcgcgggacc atgcgcttcg ggaccttctt
      541 cttcttccag gccgcccccg ggctcagcca cgccgaggtg gtccaccgcg agctcgagca
      601 gatggagtgg acggaggagc tcggcttcga cgagatctgg ctgaccgagc accacttcat
      661 cgactacggc ctggcggtgg atccggcgag cctcgccgcc accgcggccg cgcgcacccg
      721 ccgggtccgc atcgggctgg ccgccgcgat cctgcccttc caccatccgc tgcggctggc
      781 cgagcagatg gccctcatcg acatcatctc gaagggccgc ctcgacgtgg gcgtcgggcg
      841 gggcaatcgc ccggccgagt tccgcggcta ccacgtgccc cagcaggaga accgcgagcg
      901 cttcgacgag gcggtcgaca tcatcctgaa ggcgtggacg caagagcggt tcacgcacga
      961 gggccgcttc tacaccatcg gcgaggcgcg ggtgatcccc aagccctggc agcggccgca
     1021 cccacccatc taccaggtgt gcggcagcga cgacggcatc gagtcctccg ccgcgcgcgg
     1081 ctggccgatg ctcaactcgc tgctcaccgg gccggtggac cagctgatca agcgccgcga
     1141 cagctacctc accgcgctgc gcaagcacgg ccgcaccgag cccgagatca ccaccctcat
     1201 gaaggactgg ggcgtgtcgc gccagatcta cgtggcggac accgacgccc aggccttggc
     1261 cgaggccaag gacgccgaga tgtggtacca ggactcgctg cgccggttcc aggtgcccga
     1321 gcgcatcgag gacgcgcacc cgagcctgca gcccggcttc cgcgcggcgg ccgagcgctt
     1381 caagaaggtc acctgggagg ggctggtggc ggagacggtg gccttcggct cgccggacac
     1441 ggtggcgcgc cacatccaga ccatgcgcga catgggcgtc ggccacgtgc tctgctggat
     1501 gaacttcggc gggctgcccc aggaccggat ccggcgctcc atggagctgt tcgcgcgcga
     1561 ggtcatgccg cacttccgcg gctgaccgcc cgcggggcta tactggccgg agagatggcc
     1621 tacaacgcgt ggttccagtg catcaacggc tgcccggggg agttcgacct ccgggaggtg
     1681 atctaccgct gccccacgtg cggggacctg ctcgaggtcc agcacgacat ggacgcgctg
     1741 cggtcgcgct ccgccgcggc gtggatccag ctcttcgacg accggtaccg ccgcaacatc
     1801 tacccctacg gctccggggt ctggggcaag aaggagtggg tggtgccctt cattgacaac
     1861 cagaacatcg tgtcgatgta cgagggcaac tcgaacctcc tgtgggccga ccgctacggc
     1921 aagcagctcc gcgtcgagga cctgtggatc aagcagtgcg gcaactcgca cagcggctcc
     1981 ttcaaggacc tcgggatgac ggtgctcgtc tcggtggtca aggagatgat ggcgggcggc
     2041 tcgccgatcg acgcggtcgc ctgcgcctcc accggcgaca cctccgccgc gctgggcgcc
     2101 tactgcgcgg cggcgggcat cccctcggtg gtgctcctgc ccaaggacaa gatctcgatc
     2161 gcccagctcg tgcagccgct cgccaacggg gcgctggtcc tgtcgctcga caccgacttc
     2221 gacgggtgca tggcgatcgt gcaggagatc acccgggaga agcggatcta cctggccaac
     2281 tcgatgaaca gcctgcgcat cgaggggcag aagacggtgg ccatcgagat cgtccagcag
     2341 ttcgactggg aggtgccgga ctggatcgtg atcccgggcg gcaacctcgg caacgtgcac
     2401 gcgctcgggc agggctttct catgatgcag gagctggggc tggtcaaccg gctgccgcgc
     2461 atctgcctgg cccaggccga gcgggccaac ccgctctacc gctcgttcca gaacgacttc
     2521 aagaccttcg agccgatcac cgcccagccc accctggcca ccgcgatcca gatcggcaac
     2581 ccggtgtccg tcaagcgcgc gatccggacg ctccagcgct tcaacggggt ggtcgagcag
     2641 gccaccgagc aggagctcgc cgacgaggcg gcccgcgccg accgcaccgg catgttcaac
     2701 tgcccccaca ccggcgtggc gctggccgcg ctgcggaagc tggtggaccg caaggtcatc
     2761 cgctccgggg agcgcgtggt ggtcctctcg accgcgaacg ggctcaagtt cgccgaccag
     2821 aaggcggcct atcacatggg cacactcgag ggcatcaagc cgtcctaccg caacaagccg
     2881 gtcgagctgc ccgccgacgc caagcgcgtc gcccggacca tcgcgcgcca cgtcgagaag
     2941 gcgcgcgccc tggtgtcctg atggccgacc gtccggagcc ggggccggtg gatccgtcgc
     3001 ggaagccggt cggccccagc accctctcgg tgcacggggg tgagccgcgg ccgaagccgg
     3061 cgcacgcgct cgcgaccccg atcgtgcaga ccgcgaccta caccttcagc agcacccagg
     3121 agctgaagga ccacttcgac ggcaagatcg agcgcgtcga gtacgggcgc tacggcaacc
     3181 ccacccagcg catcgcggag gccaagctgg ccgcgctcga gggcgcggga gactgcctgc
     3241 tcttcggcag cggcatgggc gcgatgacca ccgcgctctt cgcgatgctc tcgcgcggca
     3301 cccacgtggt ggtgaccgac gacagctacc ggcgcacccg ccagttcctg aaccagaccc
     3361 tctcgcgcta cgggatcgag gtgtccacgg taccggcggg ggactacgag gcgctcgagg
     3421 acgcgatcaa gcccaccacc cgcgtgctgg tgagcgagtc gccgaccaac ccgtacaacc
     3481 gcatcctcga cctcgagcgg ttcgcgacta tcggccgatc tcatcgcgtc aagaccgtga
     3541 tcgacgccac cttcgcgacg ccctacaacc agcgcccgct cgagtggggg gtcgacgtgg
     3601 tggtccactc cgccaccaag tacctcggcg gccacaacga cctgctggcc ggggcggtgc
     3661 tgggctcggc cgagctggtg ggcgcggtcc gggggctgca gggcatcacc ggcgcggtgc
     3721 tcgatccctt cgcggcctac ctgctcgtgc gggggctcaa gaccttcgcc ctgcgcatgg
     3781 agcgccagaa cgcgaatgcc caggcggtgg ccgagttcct cgccggccac ccgcgcatcg
     3841 cggcggtgca ctacgcgggt ctctcgaccc accccgagca cgcggtcgcg cgcaagcaga
     3901 tgcgcggctt cggcggggtg gtctccttcg aggtcgcggg cgatctcgat gcggcgaccc
     3961 gggtggtgga cg
//
LOCUS       DATMCV010000231         3476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_11902, whole genome shotgun sequence.
ACCESSION   DATMCV010000231 DATMCV010000000
VERSION     DATMCV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11902"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VNU03_05410"
     CDS             complement(<1..181)
                     /locus_tag="VNU03_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73927.1"
                     /translation="MALGRALALVLLVCLASLTPLAYASPPDPTWMDGFFDDLDFDDV
                     VVSISWAAWAVEADPL"
     gene            260..1264
                     /locus_tag="VNU03_05415"
     CDS             260..1264
                     /locus_tag="VNU03_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010212491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP73928.1"
                     /translation="MADPQGIDVHAHFFSERFFRAIEQDGAASGGQIDRSNPIGPAIV
                     IKGGSSAPLEPRYWDLAKRVASMNKQGVAVQALSLTAPMVYWGQGDFGARLAREWNDG
                     ASAAHTAHPDRFVVCATLPMQDTAQALAELDRAARLPGVRAVYMGTNVNGRNLSDPAF
                     FPVFERCQALGLPIGLHPFNVNGAERLKAYYLTNLLGNPYDTGIAAAHLIFGGVLDRL
                     PKLQVILPHAGGTFPLVWGRLQRGQKVRPEANKAAKKPVKAYLRRFTYDTISHDAGAL
                     RYLINTVGADRVMLGTDFCFDMGYEKPIDVIRDNVVKLSRADQAKVVRENAARLLRLR
                     "
     gene            1281..2141
                     /locus_tag="VNU03_05420"
     CDS             1281..2141
                     /locus_tag="VNU03_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73929.1"
                     /translation="MSTEFWIAQSFNGISYGALLFLLASGLSLIFGVMRIVNLAHGSY
                     FMLGGYVGLTVALRTRSFTLACLAAAVAIGLVGVGMERFFLRRLQGQVLGQVLMTIGF
                     ALIFQDLALLIWGGDPYTVPIPALLTGTTRLGGAVFPIYRIFIVGVAIVVGLALWLVL
                     DRTRVGAMIRAAVDDAEMAQGVGINVPRVSLAVFALGAALAALGGVIGGGFLGVYPGA
                     DFEVLPYAFVVVIVGGLGSLPGAVAGSLLVGLLDNFGKALFPELSYFTLFAPMALILA
                     IRPTGLFGRG"
     gene            2138..3061
                     /locus_tag="VNU03_05425"
     CDS             2138..3061
                     /locus_tag="VNU03_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP73930.1"
                     /translation="MIARRALLAALVVAALAVVAPLLPAYPLTLLTQALIVGILAMSL
                     DVMLGYTGLSSLGHAAYFGVGAYAVAIMATEKQAGLVACLVVGVLLAAGTAAVFGLLA
                     IRATGTYFLMITLALGMVIWGLAFRWVSLTKGDNGIAGVPRPSLGALDLSGPVPFFYF
                     CLVSATVAWALMGLLVVSPFGLGLRGIRGSETRMQALGYNVWLHKYAAFVISGVFAGF
                     GGTLWAYYNGFVSPNDVQLVTSVETLLMVALGGPGTLIGPALGAGIIVFLKNFVSVYT
                     KRWLLILGGVYIGVILFAPSGIVGALRGRRR"
     gene            3193..>3476
                     /locus_tag="VNU03_05430"
     CDS             3193..>3476
                     /locus_tag="VNU03_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP73931.1"
                     /translation="MDGVTLEVRPGERRALIGPNGAGKTTLFNLISGAMPPSAGRISL
                     FGQDVTGAPAHRRAALGLARTFQITNLFPDLSVLENCLLAVQAHTPTRFA"
BASE COUNT          448 a         1282 c         1173 g          573 t
ORIGIN      
        1 caagcggatc cgcttcaacc gcccaggcgg cccacgagat ggagacgacg acgtcgtcga
       61 agtccaggtc gtcgaagaac ccatccatcc atgtcggatc gggcgggctg gcgtaggcaa
      121 gcggagtcag gctggcgaga cagacaagaa gaacaagcgc gagcgctctc ccgagtgcca
      181 tcggacggga gcctacgccc gccgctgcgc ccggtcaaga cgccgtatgg cgccgggatt
      241 ctatagtaga atccccgccg tggccgaccc ccagggcatc gacgttcacg cgcacttctt
      301 ctccgagcgc ttcttccgcg ccatcgagca ggacggcgcc gcgtccggcg gccagatcga
      361 ccgcagcaac ccgatcggcc ccgccatcgt gatcaagggc ggcagcagcg cgccgctcga
      421 gccgcgctac tgggacctgg ccaagcgcgt ggcctccatg aacaagcagg gcgtggccgt
      481 ccaggccctc tcgctcaccg cgccgatggt ctactggggg cagggcgact tcggtgcccg
      541 gctcgcgcgc gagtggaacg acggcgccag cgccgcccac accgcccatc ccgaccgctt
      601 cgtggtctgc gccaccctgc ccatgcagga caccgcgcag gccctggccg agctggaccg
      661 ggccgcgagg ctgcccggcg tgcgcgcggt ctacatgggc accaacgtca acgggcgcaa
      721 cctctcggat cccgcgttct tcccggtgtt cgagcgctgc caggccctcg gcctgccgat
      781 cgggctgcac ccgttcaacg tcaacggggc ggagcggctc aaggcctact acctgaccaa
      841 cctgctgggc aacccgtacg acaccggcat cgcggcggcg catctcatct tcggaggtgt
      901 gctggaccgg ctgccgaagc tgcaggtgat cctgccgcat gcgggcggca cctttccgct
      961 ggtgtggggg cgcctccagc gcgggcagaa ggtgcggccg gaggcgaaca aggcggccaa
     1021 gaagccggtg aaggcctacc tgcgccgctt cacctacgac acgatcagcc acgatgcggg
     1081 ggcgctgcgc tacctcatca acaccgtggg cgcggaccgg gtgatgctcg gcaccgactt
     1141 ctgcttcgac atgggctacg agaagcccat cgacgtcatc cgcgacaacg tggtcaagct
     1201 ctcgcgggcc gaccaggcca aggtcgtgcg cgagaacgcc gcccggctgc tgcgcctccg
     1261 ctgagagccg ggctcggggc gtgagcaccg agttctggat cgcgcagtcg ttcaacggca
     1321 tctcctacgg cgcgctcctc ttcctcctgg cgagcggcct ctcgctcatc ttcggcgtca
     1381 tgcgcatcgt caatctcgcg cacggctcct acttcatgct gggcggctac gtggggctca
     1441 ccgtggcgct gcgcacgagg tcgttcacgc tggcgtgcct ggccgccgcg gtggccatcg
     1501 ggctggtggg cgtcggcatg gagcgcttct tcctccgacg cctgcagggc caggtgctgg
     1561 gccaggtgct gatgaccatc ggcttcgcgc tgatcttcca ggacctggcg ctgctcatct
     1621 ggggcgggga tccctacacg gtgccgatcc ccgcgctgct caccggcacc acccgcctgg
     1681 gcggcgcggt gttcccgatc taccgcatct tcatcgtcgg cgtggccatc gtggtgggcc
     1741 tggccctctg gctggtgctc gaccgcacgc gcgtgggcgc gatgatccgc gccgcggtgg
     1801 acgacgcgga gatggcccag ggcgtcggca tcaacgtgcc gcgggtgtcg ctggccgtct
     1861 tcgcgctcgg cgccgcgctg gccgcgctgg gcggggtgat cggcggcggc ttcctcggcg
     1921 tgtacccggg ggcggacttc gaggtcctgc cctacgcgtt cgtggtggtc atcgtgggcg
     1981 ggctcggcag cctgcccggc gccgtcgcgg gcagcctgct ggtgggcctg ctcgacaact
     2041 tcggcaaggc gctcttcccc gagctgtcct acttcacgct tttcgcgccc atggcgctga
     2101 tcctcgccat ccgaccgacg ggcctcttcg gccgcggatg atcgcgcgcc gcgcgctgct
     2161 ggccgccctg gtcgtggcgg ccctggccgt ggtggcgcca ctgctgccgg cctatccgct
     2221 gacgctcctc acccaggcgc tcatcgtggg catcctggcc atgagcctcg acgtgatgct
     2281 gggctacacc ggtctctcgt cgctgggcca cgcggcctac ttcggcgtcg gcgcctacgc
     2341 ggtcgcgatc atggccaccg agaagcaggc cgggctcgtg gcctgcctgg tggtgggcgt
     2401 gctgctggcc gcgggcaccg ccgccgtctt cggcctgctc gccatccgcg ccaccggcac
     2461 ctacttcctg atgatcacgc tggcgctcgg catggtgatc tgggggctcg cgttccgctg
     2521 ggtctcgctc accaagggcg acaacggcat cgccggcgtg ccgcggccgt cgctcggggc
     2581 gctcgatctg agcgggcccg tgcccttctt ctatttctgt ctcgtctcgg ccacggtggc
     2641 gtgggcgctg atggggctgc tggtggtctc gcccttcggc ctcggcctgc gcggcatccg
     2701 cggcagcgag acgcgcatgc aggcgctggg ctacaacgtg tggctgcaca agtacgcggc
     2761 cttcgtcatc tcgggcgtgt tcgcgggctt cggcggcacg ctctgggctt actacaacgg
     2821 cttcgtcagc ccgaacgacg tgcagctcgt cacctcggtg gagaccttgc tgatggtcgc
     2881 gctcggcggc ccgggcacgc tgatcggccc cgcgctgggc gccggcatca tcgtgttcct
     2941 gaagaacttc gtcagcgtct acaccaagcg ctggctgctc atcctgggcg gcgtctacat
     3001 cggggtgatc ctcttcgcgc ccagcggcat cgtcggcgcc ctccgaggcc gccgccgatg
     3061 acgcagaatc cccccctctc ccccccggcg agagggcagg gtgaggggcc cagaggcgag
     3121 gggcccagag gtgaggggcc ccccgccctc cgcctcgaca acgtctgccg ctccttcggc
     3181 ggtctccagg ccgtggacgg cgtcaccctc gaggtgcgcc ccggcgagcg ccgcgccctc
     3241 atcggcccca acggcgcagg caagaccacg ctcttcaacc tgatctccgg cgccatgccc
     3301 ccgagcgccg gccgcatctc cctcttcggc caggacgtca ccggcgcccc cgcccaccgc
     3361 cgcgccgcgc tcggtctcgc ccgcacgttc cagatcacca atctcttccc cgacctgagc
     3421 gtgctggaga actgcctgct cgcggtgcag gcccacaccc cgacgcgctt cgcgat
//
LOCUS       DATMCV010000232         2614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_346182, whole genome shotgun sequence.
ACCESSION   DATMCV010000232 DATMCV010000000
VERSION     DATMCV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346182"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VNU03_05435"
     CDS             <1..791
                     /locus_tag="VNU03_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HWP73932.1"
                     /translation="ARGGLMRAPAIRALGLLSGAGPGSGPAPIWTLPRPERAGDRFRR
                     ATRECLLALAAVDAMLEDGKAPREAIAGDRTALLYVTAAAYGASNREFIERRGGVTHF
                     AYTAPAVVPAEVAIEFGVTGEYGILIGGAPSTLRAIDQAARLLDEGRCDRALLLVVEI
                     FEECADLYTRHRRFTRWPLVETAACLWLEPGAGELRFESARGGKPAAATRGGERFAAG
                     PLADLREWLDRAPAGPIELSGRWRDEHARLHWSTERSHARGSAA"
     gene            808..1083
                     /locus_tag="VNU03_05440"
     CDS             808..1083
                     /locus_tag="VNU03_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWP73933.1"
                     /translation="MTQKEVLDELKGLVVERLKFDPRRAAEMTLETTLPKGVEGSLGL
                     DSLDFIELSVALEERFGFVIDEGHDLTDEFLSLDSLSRFILAKIGQA"
     gene            1212..2396
                     /locus_tag="VNU03_05445"
     CDS             1212..2396
                     /locus_tag="VNU03_05445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HWP73934.1"
                     /translation="MSGLGIVSPYGAGVKSFWTGLAAGTCAIKPITLIDTEGFRSRIA
                     AEVPAEVVATLGVSRRRSRADRLALAAAREALADAGLEGAARRRAALLIGAVGGGMLE
                     GENWYWGEARGRPAGSIRALRSILPLSHAETLGGRLGLTGPKETVVMACASGAASIAL
                     GADLVRAGVTPTALVGGVDALTHICFMGFNALKVLDPTPCRPFDRDRRGMSIGEAAAF
                     LVVEDADHCRARGGRVHARLAGYGMTTDAHHVTAPHPEGEGMIHAMEQALADAGLEPS
                     AVGYVNAHGTGTPQNDRAEALALRRVFGEGGVLVSSTKSLVGHTMAAAGSVEAVATIL
                     ALQHGLLPPTAGLEHLDPEVPFDCLPITARPAEVGAALSNSFGFGGQNVSLVFERAGG
                     RA"
     gene            2389..>2614
                     /locus_tag="VNU03_05450"
     CDS             2389..>2614
                     /locus_tag="VNU03_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73935.1"
                     /translation="MPDDRRDRDLRVVVTGLGTINASTAGGREALAGALALGRSAIGP
                     VRAFATAGCASGLAAEVDEATLTSLVDRDAA"
BASE COUNT          308 a          931 c         1025 g          350 t
ORIGIN      
        1 gtgctcgagg gggtttgatg cgcgcccccg ccatccgcgc cctgggcctc ctgagcggcg
       61 ccgggccggg ctcggggccg gccccgatct ggacgctccc gcgaccggag cgggcggggg
      121 accgcttccg gcgggccacc cgcgaatgct tgctggctct tgcggccgtc gacgcgatgc
      181 ttgaagatgg gaaggcgccg cgggaggcga tcgcgggaga ccggaccgcg ctcctgtacg
      241 tgaccgcggc cgcgtacggg gcgtcgaacc gcgagttcat cgagcggcgg ggcggcgtga
      301 cgcacttcgc gtacaccgcc ccggcggtgg tgcccgcgga ggtggccatc gagttcgggg
      361 tgaccgggga gtacggcatc ctgatcggag gggccccgtc gacgctgcgc gcgatcgacc
      421 aggcggcacg gctcctggac gaggggcgct gcgatcgggc gctgctgctg gtggtggaga
      481 tcttcgagga gtgtgcggat ctctacaccc gtcatcggcg tttcacgagg tggccgctgg
      541 tcgagaccgc cgcgtgtctc tggctcgagc ccggcgcggg cgagctgcgg ttcgaatcgg
      601 cgcggggcgg gaagcccgcc gcggcgaccc gcgggggcga gcgcttcgcg gcgggcccgc
      661 tcgcggacct gcgcgagtgg ctcgaccgcg cgccggccgg accgatcgag ctgtcgggac
      721 gctggcgcga cgagcacgcg cggcttcact ggagcacgga gcgatcccac gcgcgcggca
      781 gcgcggcgtg atagggaggt cgaggcgatg acgcagaagg aagtgctgga cgagctgaag
      841 gggctcgtcg tcgagcggct gaagttcgat ccgcgccgcg cggccgagat gaccctggag
      901 acgacgctgc ccaagggtgt cgagggctcg ctcggcctgg actcgctcga cttcatcgag
      961 ctgtcggtgg cgctcgagga gcgcttcggc ttcgtgatcg acgagggcca cgacctcacc
     1021 gacgagttcc tctcgctgga ctccctgtcg cggttcatcc tggcgaagat cggccaggcc
     1081 tgagcgcggg agcgcgcggt ccgaccgatg gacgcgccat gagccggccg cccggtagcc
     1141 gtcggccccc cggtagctgt cggctccccg gtagccgtcg gccccccggt agccgtcggc
     1201 ccgaggtcgt ggtgagcggg ctcggcatcg tgagccccta cggcgcgggc gtcaagagct
     1261 tctggaccgg cctcgcggcc ggcacctgcg cgatcaagcc catcaccctg atcgacaccg
     1321 agggcttccg ctcgcgcatc gcggccgagg tgcccgccga ggtcgtggcc actctcgggg
     1381 tctcgcgacg gcgctcgcgc gcggaccggc tcgcgctcgc cgccgcgcgc gaggcgctgg
     1441 ccgacgccgg gctcgaggga gcggcgcggc ggcgcgccgc gctgctcatc ggggcggtcg
     1501 gcggcggcat gctcgagggc gagaactggt attggggcga ggcgcgcggg cggcccgccg
     1561 gcagcattcg ggccctgcgc tcgatcctgc cgctctccca cgccgagacg ctcggcgggc
     1621 ggctcgggct gaccgggccg aaggagacgg tggtcatggc ctgcgcgtcg ggcgcggcct
     1681 ccatcgcgct cggggccgac ttggtgcggg cgggcgtcac gccgaccgcg ctggtgggcg
     1741 gggtggacgc gctgacccac atctgcttca tgggcttcaa cgcgctcaag gtgctcgatc
     1801 ccactccgtg ccgccccttc gaccgggacc gccggggcat gtcgatcggc gaggccgcgg
     1861 ccttcctcgt cgtcgaggac gccgatcact gccgggcccg cggcgggcgc gtgcacgcgc
     1921 ggctcgccgg ctacgggatg accaccgacg cccatcacgt gaccgcgccg cacccggaag
     1981 gcgagggcat gatccacgcg atggagcagg cgctcgccga cgcgggccta gagccctccg
     2041 cggtgggcta cgtcaacgcc cacggcaccg gcaccccgca gaacgatcgc gcggaggcgc
     2101 tggccctgcg acgcgtcttc ggcgagggtg gggtgctggt cagctccacc aagtcgctcg
     2161 tgggccacac catggccgcg gcggggagcg tggaggccgt cgcgaccatc ctggcgctcc
     2221 agcacggcct gctgccgccc accgcgggcc tcgagcacct ggatcccgag gtgccgttcg
     2281 actgcctgcc gatcacggcg cggcccgccg aggtgggcgc ggcgctctcg aactccttcg
     2341 ggttcggcgg ccagaacgtg agcctcgtct tcgagcgggc gggcggccgt gcctgacgat
     2401 cggcgtgacc gcgaccttcg agtcgtcgtc accggcctcg gcaccatcaa cgcctccacc
     2461 gcgggcgggc gggaggcgct ggcgggcgcg ctcgccctcg ggcgctccgc gatcggcccg
     2521 gtgcgggcct tcgcgacggc gggctgcgcg agcggcctcg cggccgaggt cgacgaggcc
     2581 acgctgacct cgctggtgga ccgcgacgcg gccc
//
LOCUS       DATMCV010000233         6056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_211957, whole genome shotgun sequence.
ACCESSION   DATMCV010000233 DATMCV010000000
VERSION     DATMCV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211957"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1438)
                     /locus_tag="VNU03_05455"
     CDS             complement(56..1438)
                     /locus_tag="VNU03_05455"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HWP73936.1"
                     /translation="MPRAALVLGLALALAAALPGTAAPADPELLVTVGEVTEASAVVW
                     VRGVAWGEVTVRYAPVIRAPDGAAGPPVSARGQIRVAPSHNLTGKLVLQPLEPATRYR
                     YTITQNAAATSGEFVTAPAPTVAIPVRFAWSGDLGSRGHCRKPGGVFPVFRALAQVPA
                     DFFLFVGDTIYADHECGESTRVPGYDFVARRLADFWAKHLYNRRDPAGQAYFRSTSVY
                     AIWDDHEVRNDFSGPSEPLTETGRRAFIDYFPIRPPREEPGRLYRSFRWGSLLEVFIL
                     DTRQYRSPNTDPDGPGKTMLGAAQRRWLIDAVSSSTAVWKVVISSVPLSVPTGSHGHD
                     SWSNANAQGVPEERSTGFAVERDSILRVLRQRGVRNLVVLAADVHFAQLIRHQPTTDW
                     SFHEFIAGPLSATPGRPRPLDQGLNPRSLWSLGGTATFGDIAIDTGGLTVRIVDGDGR
                     ERFVQTIGAE"
     gene            complement(1461..2369)
                     /locus_tag="VNU03_05460"
     CDS             complement(1461..2369)
                     /locus_tag="VNU03_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP73937.1"
                     /translation="MLKDKVAVVTGAARGIGREIALLLAKHGAKVVVNDYGGGSDGAG
                     DARGPADDVVNEIKGQGGQAAASYDSVASMAGGQRIVQTALDHFGHVDIVVNNAGILR
                     DRMIFNMTEEEWDGVINTHLKGTFAVTRAAVPHMRERKWGRIINMTSTSGLVGNIGQA
                     NYAAAKLGIVGFTKVVALDMARYNVTANCISPFAWTRMIGTIPTETEAQKARVEKIKK
                     MGPEHIAPVAVFLASDEAKEISGQVFGVRGKEVMLFSHMRPVRSIHHDLGWTPERLAA
                     MFPGTLKHHLAPLDTSGQFFNYDPMV"
     gene            complement(2437..3639)
                     /locus_tag="VNU03_05465"
     CDS             complement(2437..3639)
                     /locus_tag="VNU03_05465"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP73938.1"
                     /translation="MADHLPPPLSGVRVLELAHLIAGPICGMYLADMGADVVKVESRE
                     APDAGRSVYPRAVRNGEGVLHLTVNRNKRGICLDVKQPEGRDAFLRLAGWADVIIEGF
                     RGGVAERLGIDYDSVKGANPRLIYCSISAFGPDGPWREKPGLDSLAQALGGLMAITGE
                     PAGGPVLCGAPVADTLGGMLAIQGILTALISRAATGQGQRVDASLLNGMLFAHTARLS
                     VFHETGEPLPRYGSGHPEIVPYQAFEAADGWIFVAAWVDRLWLPFCKAVGVDALATDP
                     RFATRKDRLTHRGELEAILAPVFRARPVAQWMETLEQADVLCAPVNDYPGLVRHPQVV
                     ATGFITEQAHPRAGRFKTVATPVKLEKTPGTIRTPAPLLGEHSRDVLAEAGFAPAEIE
                     SLAARGVI"
     gene            complement(3666..4469)
                     /locus_tag="VNU03_05470"
     CDS             complement(3666..4469)
                     /locus_tag="VNU03_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWP73939.1"
                     /translation="MIGAFRTALASFRDRRGMFLASGLAFNFVVCLIPLLFFVVSVAG
                     FVLSRRAVMEAVLNQLSAIVPVYKEELHQNLAVIIRRRRLSGLLGTAVLLLFASQLFN
                     SIRLVLSDIFGFRGGPGFLRGMLRDMVMVLAMGLLFLASIVISDVFGWLKILVFQTLG
                     MPTQWVRSWSLALALALSTAFFFVPYRYFPHRRVPVGAALAGALLAAVLWETAKQLFR
                     LYILSFGVYDQVYGPLGVLVALAMFAYYSGVVFILGAEFAAALMKGGRA"
     gene            complement(4496..5287)
                     /locus_tag="VNU03_05475"
     CDS             complement(4496..5287)
                     /locus_tag="VNU03_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP73940.1"
                     /translation="MDLGIKGKTALVVAASKGMGRASALGLAAEGARVVMCARGEAAL
                     KDAAAEVKQKTGAEVLALTADASRAADISKVVAEANRAFGGVDILVANVGGPPPGPFE
                     AMTDEHWKTAFEQVHLSTVRFIREVLPHMKQARWGRILAIQSSSVKQPVDGLVLSNGI
                     RPGIAGLFKTLAGDLAPHGITVNLVLPGRIMTDRFREHQTDRATRGGVTLEKQMELSS
                     ADIPMGRIGTPEEFAAMVVFLASARASYVTGTAVQVDGGLIRSVV"
     gene            complement(5307..>6056)
                     /locus_tag="VNU03_05480"
     CDS             complement(5307..>6056)
                     /locus_tag="VNU03_05480"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HWP73941.1"
                     /translation="YVVMGGETIQVTFGKGRAMRAELVAQDFEVGLALLRVKRQGLPA
                     VPIADSQSIDRGTPVFALGSTGPRERRVAGGLVTYLGEYAGYWEYLLDRGIVSSAANP
                     GFGGGPLFTLTGQMVGVVSLNLNEIARCSLAIPTECYRRNQEEFMRFGRVVSRPQRAW
                     LGVFAHALEEGVVVAGLVPNGPGARSGIQEGDLIVSLDAQEVATPKELYLSLWKHTPG
                     EKMTLEVMRDNEVRRLSVTGGDRADFYKQSS"
BASE COUNT          905 a         2134 c         2159 g          858 t
ORIGIN      
        1 ggggatctac ctcgtcaacc ggcgcgcctg atccgcgccg tcactccgct ccgccctact
       61 ccgctccgat agtctgcacg aagcgctcgc gcccgtctcc gtcgacgatg cgcacggtga
      121 ggccgccggt atcgatggcg atgtccccga aggtcgcggt tccgcccagg ctccacagcg
      181 agcgcgggtt gaggccctgg tcgagcgggc gcggccgacc cggggtggcc gagagcgggc
      241 ccgcgatgaa ctcgtggaag gaccagtcgg tagtcggctg gtgacggatc agctgcgcga
      301 agtgcacgtc ggcggcgagg acgacgaggt tccggacccc gcgctgccgc aggacgcgga
      361 ggatggagtc gcgctcgacc gcgaaaccgg tggagcgctc ctccggcacc ccctgggcgt
      421 tggcgttgct ccaggagtcg tgcccgtggc ttccggtggg caccgacagg ggcacgctcg
      481 atatcaccac cttccacacc gcggtcgatg acgacaccgc gtcgatcagc cagcgccgct
      541 gcgcggcgcc cagcatggtc ttgcccggtc cgtcggggtc cgtgttgggg ctccggtact
      601 gccgggtatc caggatgaag acctcgagga gcgagcccca gcggaagctg cgatagagtc
      661 ggcccggctc ctcccgggga ggccggatcg ggaagtagtc gatgaaggcg cgccggccgg
      721 tctcggtgag cggctcggag ggacccgaga agtcgttccg cacctcgtgg tcgtcccaga
      781 tcgcgtagac cgaggtggac cggaagtagg cctggccggc ggggtcccgt cggttgtaga
      841 ggtgcttggc ccagaagtcg gcgagccggc gcgcgacgaa gtcgtagccc ggcacgcgtg
      901 tcgactcgcc gcattcatgg tcggcgtaga tggtgtcgcc cacgaagagg aagaagtcgg
      961 ccggcacctg ggcgagggcg cggaagaccg ggaagacgcc gcccggcttg cggcagtggc
     1021 cgcggctgcc caggtcgccg ctccacgcga agcggaccgg gatcgccacg gtgggcgcgg
     1081 gtgcggtcac gaactcgccg gaggtcgcgg ccgcgttctg ggtgatcgtg tagcggtagc
     1141 gggtggccgg ctcgaggggc tggagcacga gcttgccggt gaggttgtgg ctcggggcca
     1201 cccggatctg accgcgcgcg ctcaccgggg gccccgccgc cccgtcggga gctcggatca
     1261 ccggcgcgta gcgcaccgtg acctcgcccc acgcgacgcc gcgcacccag accaccgcgc
     1321 tggcctcggt gacctcgccc accgtgacca gcagctccgg gtcggcgggg gcggccgtcc
     1381 ccgggagcgc cgcggccagt gctaaggcca ggccgagcac gagtgccgcg cggggcatcg
     1441 ctccgcgccc cgctcccggc tcagaccatc gggtcgtagt tgaagaactg acccgaggtg
     1501 tcgagcggcg ccaggtggtg cttgagcgtg ccagggaaca tggcagccag gcgctccggc
     1561 gtccacccga ggtcgtggtg gatgctccgc accggccgca tgtgcgagaa cagcatgacc
     1621 tccttgccgc gcaccccgaa gacctgcccc gagatctcct tggcctcgtc ggaggcgagg
     1681 aacaccgcga ccggcgcgat gtgctcgggg cccatcttct tgatcttctc gacccgcgcc
     1741 ttctgggcct cggtctcggt ggggatggtg ccgatcatgc gcgtccacgc gaagggcgag
     1801 atgcagttgg cggtcacgtt gtagcgggcc atgtcgagcg ccaccacctt ggtgaagccc
     1861 acgatgccga gcttggccgc cgcgtagttg gcctggccga tgttgcccac gaggcccgag
     1921 gtcgacgtca tgttgatgat gcggccccac ttgcgctccc gcatgtgggg caccgcggcg
     1981 cgggtcaccg cgaaggtgcc cttcagatgc gtgttgatga cgccgtccca ctcctcctcg
     2041 gtcatgttga agatcatccg gtcgcggagg atgcccgcgt tgttcaccac gatgtcgacg
     2101 tgcccgaagt ggtcgagcgc ggtctggacg atgcgctggc cgcccgccat gctcgccacc
     2161 gagtcgtagc tggccgcggc ctgcccgccc tgccccttga tctcgttgac cacgtcgtcg
     2221 gccgggccgc gcgcgtcgcc ggccccgtcg gagccgccgc cgtagtcgtt cactaccacc
     2281 ttggccccgt gcttcgccag gagcagcgcg atctcgcggc cgatgccgcg cgctgcgccc
     2341 gtcaccaccg ccaccttgtc cttgagcatc atggaagtct cctaacctcg tctatattcg
     2401 ctcggctcgc cttcggctgc acctcgccca gctcggtcag atgacgccgc gtgcggcaag
     2461 cgattcgatc tccgccggcg cgaagccggc ctcggcgagc acgtcccggc tatgctcgcc
     2521 cagcagcggc gcgggagtgc ggatggtgcc cggcgtcttc tcgagcttga ccggtgtcgc
     2581 caccgtcttg aagcgtcccg cgcgcgggtg cgcctgctcg gtgatgaacc cggtggcgac
     2641 cacctggggg tgccggacca gccctggata gtcgttgacc ggcgcgcaga gcacgtcggc
     2701 ctgctcgagg gtctccatcc actgggcgac cgggcgcgcc cggaacaccg gggccaggat
     2761 cgcctcgagc tcgccgcgat gggtgagccg gtccttgcgg gtggcgaagc gcggatcggt
     2821 ggcgagcgcg tcgacgccga ccgccttgca gaacggcagc cacaggcggt cgacccacgc
     2881 agccacgaag atccagccgt ccgcggcctc gaaggcctgg tacggcacga tctcgggatg
     2941 cccgctgccg tagcgcggca gcggctcccc ggtctcgtgg aacaccgaga ggcgcgcggt
     3001 gtgcgcgaag agcattccgt tcaggagcga cgcgtccacc cgctgcccct gcccggtcgc
     3061 ggcccgcgag atcagcgcgg tgaggatgcc ctggatggcg agcatgccgc cgagggtgtc
     3121 ggccaccggc gccccgcaga gcaccgggcc gcccgccggc tcgccggtga tggccatgag
     3181 gccgcccagg gcctgcgcga gggagtcgag gcccggcttc tcgcgccagg ggccgtcggg
     3241 accgaaggcg gagatcgagc agtagatcag gcgcgggttc gctcccttca ccgagtcgta
     3301 gtcgatgccc aggcgctcgg cgactccgcc ccggaagccc tcgatgatga cgtcggccca
     3361 cccggccagg cggaggaagg cgtcgcgccc ctcgggctgc ttgacgtcga ggcagatccc
     3421 gcgcttgttg cggttgacgg tcaagtgcag cacgccctcg ccgttgcgca ccgcgcgcgg
     3481 gtacaccgac cgtcccgcgt cgggcgcctc gcgcgattcc accttgacca cgtcggcgcc
     3541 catgtcggcc aggtacatcc cgcagatggg tcccgcgatc aggtgagcca gctcgaggac
     3601 ccgcacgccg gagagcggcg gaggcaggtg gtcggccatg accggatcac cctagcacag
     3661 accggtcagg cccggccgcc cttcatcagc gcggcggcga actcggcgcc caggatgaag
     3721 accaccccgg agtagtaggc gaacatggcc agggccacca gcacgcccag gggcccgtag
     3781 acctggtcgt agaccccgaa ggagaggatg tagaggcgga agagctgctt ggccgtttcc
     3841 cacagcaccg cggcgagcag cgcgccggcc agcgccgcgc cgaccggcac ccggcggtgg
     3901 gggaagtagc ggtagggcac gaagaagaac gcggtgctca gcgccagcgc gagcgcgagc
     3961 gaccaggagc gcacccactg cgtcggcatc cccaaggtct ggaacacgag gatcttgagc
     4021 cagccgaaga cgtcgctgat cacgatgctg gcgaggaaca ggagtcccat cgcgaggacc
     4081 atcaccatgt cgcgcagcat gccgcggagg aacccggggc cgccgcggaa cccgaagatg
     4141 tcgctcagca cgaggcggat cgagttgaag agctggctcg cgaagagcag cagcaccgcg
     4201 gtgccgagca gtccggacag gcggcgcctc cggatgatga cggcgaggtt ctggtgcagc
     4261 tcctccttgt agaccgggac gatcgcggag agctggttga gcaccgcctc catcaccgcc
     4321 cggcggctca gcacgaagcc cgccaccgac accacgaaga acagcagcgg gatgaggcag
     4381 acgacgaagt tgaaggcgag cccggaggcg aggaacatcc cgcgccggtc gcggaagctc
     4441 gccagcgcgg tccggaaggc cccgatcaca gggtggggtc cgccgtctcc ccggcctaga
     4501 cgacgctccg gatgagcccg ccgtccacct gcaccgcggt gccggtgacg taggaggccc
     4561 gcgcggaggc gaggaacacc accatcgccg cgaactcctc cggggtgccg atccggccca
     4621 tcgggatgtc ggcggaggac agctccatct gcttctccag cgtgaccccg ccacgggtgg
     4681 ctcggtcggt ctggtgctcg cggaagcggt cggtcatgat ccgtcccggc aggaccaggt
     4741 tgaccgtgat gccgtggggc gcgaggtccc cggccagcgt cttgaagagg cccgcgatgc
     4801 cgggacggat gccgttggag aggacgagcc cgtcgaccgg ctgcttcacc gagctcgact
     4861 ggatcgcgag gatacggccc cagcgcgcct gcttcatgtg cgggaggacc tcccggatga
     4921 agcgaaccgt cgagaggtgg acctgctcga aggcggtctt ccagtgctca tcggtcatcg
     4981 cctcgaatgg tccggggggc gggccgccca cgttggcgac caggatgtcg actccgccga
     5041 aggcccggtt ggcctccgcc accaccttcg agatgtcggc cgcgcggctc gcgtcggcgg
     5101 tcaaggccag cacctccgcg ccggtcttct gcttgacctc cgcggccgcg tccttgaggg
     5161 cggcctcccc gcgcgcgcac atcaccaccc gcgcgccctc ggcggccagg ccgagcgcgc
     5221 tcgcccggcc catccccttg ctcgccgcca ccaccagcgc ggtcttgccc ttgatgccca
     5281 gatccatcgg gagcctccct gtggcgtcag gaactctgct tgtagaagtc ggcgcggtcg
     5341 ccgccggtga cggacagccg gcggacctcg ttgtcgcgca tcacctcgag ggtcatcttc
     5401 tcgcccggcg tgtgcttcca gagcgacagg tacagctcct tgggggtggc cacctcctgc
     5461 gcgtcgagcg acacgatgag gtcgccctcc tggatgccgg agcgcgcgcc gggcccattg
     5521 ggcaccaggc cggccaccac cacgccttcc tccagcgcgt gggcgaagac cccgagccac
     5581 gcgcgctggg ggcggctcac cacgcggccg aagcgcatga actcttcctg gttgcgccgg
     5641 tagcactcgg tcgggatcgc gagcgagcag cgcgcgatct cgttgaggtt gagcgagacc
     5701 acgcccacca tctgcccggt cagggtgaag agcgggccgc ccccgaagcc ggggttggcc
     5761 gcgctcgaca cgatgccgcg atccaggagg tattcccagt agcccgcgta ctcgcccagg
     5821 taggtcacga gcccgcccgc cactcggcgc tcgcggggtc cggtcgagcc gagggcgaag
     5881 accggcgtcc cccgatcgat cgactgcgag tcggcgatcg ggaccgcggg gaggccttga
     5941 cgcttcacgc gcagcagggc gagcccgacc tcgaagtcct gggccaccag ctcggcccgc
     6001 atggcgcggc ccttcccgaa ggtgacctgg atggtctcgc cgcccatcac cacgta
//
LOCUS       DATMCV010000234         3270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_545237, whole genome shotgun sequence.
ACCESSION   DATMCV010000234 DATMCV010000000
VERSION     DATMCV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545237"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VNU03_05485"
     CDS             <1..879
                     /locus_tag="VNU03_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWP73942.1"
                     /translation="LGYRLLMAVSAEQALDALNVVSPDLVLTDVHLGAMSGIELCARL
                     KADPRYELMPVVILTAVGDLEARVAGLAAGADDFFTKPVEFVELRTRLAALLRVKMLL
                     GQLERAEAVITTLALTIEARDPYTLGHCDRLSRYAVALGQALGLDHEMLRALRLGGYL
                     HDLGKIAVPDGILLKPGPLDPMEQERIRAHPGAGSDLVLGLRSMELVRPIMRHHHEKW
                     DGSGYPDGLKGAAIPLGARIISVVDVFDALHTERPYKAALPRSEAVSLLIRETDAGYW
                     DPKVVETFLEILRQYK"
     gene            complement(882..1670)
                     /locus_tag="VNU03_05490"
     CDS             complement(882..1670)
                     /locus_tag="VNU03_05490"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWP73943.1"
                     /translation="MPEWLAVILLGVIEGVTEFLPISSTGHLLLVENSHLLPGQSDLF
                     NITIQSGAALAVLAVFGGRLRDLLARVREPAAGGYLAKLLLAFVITGAGGLAVKRLGF
                     VLPKEVAPIAWATLIGGVVILLVEWGLRGRSARSEVTWAVAVAVGLAQLLAAVFPGTS
                     RAAATIMIAMLLGLARPAATEFSFLVGVPTLIAAGGFETLHALRHPAGPPTKWGMLAL
                     GTVVSALTAFVVVRWLLRWIQTHSFVPFGWYRIALGLLMLARFV"
     gene            complement(1679..2497)
                     /locus_tag="VNU03_05495"
     CDS             complement(1679..2497)
                     /locus_tag="VNU03_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP73944.1"
                     /translation="MSTDASPITLRGWALVLGASSGFGAATSLALARAGMNVFGVHLD
                     RKSTLPNVERLAAEITALGRQARFFNVNAADEEKRAEVATEMERVLRERDEVGQVRVL
                     LHSLAFGTLKLFVADPIKEAVTKAQMDMTLDVMAHSLVYWAQELVGRGLMGRGGRIYA
                     MTSSGGARVLPFYGPVSAAKAALESNIRQLAAELAPHGISANSIRAGVTATPAAQKIP
                     NYEEFSQQAARRNPHRRLTTTEDVARAIVVLSHPDTYWITGNVIGVDGGEEIVG"
     gene            2657..3166
                     /locus_tag="VNU03_05500"
     CDS             2657..3166
                     /locus_tag="VNU03_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73945.1"
                     /translation="MRNVPTPVLFVLAIAALAGCAASPTVQLSSLGHEVDVHPLRGQS
                     VELLKQDDAACESWTRSTKGPNEPLPAAELRYSACALSRGYSVKPWDHRISPPASPTP
                     DLAGVVADLQACGLKRGLYDPGGLVLLGMVEAGMTEARRAEAWGCLKQRGYAVSYQPD
                     ARAESIRKK"
BASE COUNT          502 a         1168 c         1163 g          437 t
ORIGIN      
        1 ctcggctacc gcctgctgat ggcggtctcc gcggagcagg ccctcgacgc gctcaacgtg
       61 gtgagcccgg acctggtgct gaccgacgtg cacctcggcg cgatgagcgg gatcgagctg
      121 tgcgcgcggc tcaaggccga cccgcgctac gagctgatgc cggtggtgat cctcaccgcg
      181 gtgggcgatc tggaggcgag agtggccggc ctcgcggcgg gcgcggacga cttcttcacc
      241 aagccggtgg agttcgtgga gctgcggacg cgtctcgccg cgctcctgcg cgtcaagatg
      301 ctgctcggcc agctcgagcg ggccgaggcg gtcatcacca cgctcgcgct caccatcgag
      361 gcgcgcgatc cctacaccct cggccactgc gaccggctct cgcgctacgc ggtggcgctc
      421 ggccaggccc tcgggctcga tcacgagatg ctgcgggcgc tccgcctcgg tggctatctc
      481 cacgacctcg ggaagatcgc ggtgcccgac gggatcctgc tcaagccggg gccgctcgat
      541 ccgatggagc aggagcggat ccgcgcgcac ccgggggcgg gctcggacct ggtgctcggg
      601 ctgcgcagca tggagctggt gcgcccgatc atgcggcacc accacgagaa gtgggacggc
      661 tccggctatc ccgacgggct caagggcgcg gccatcccgc tcggcgcgcg catcatctcg
      721 gtggtcgacg tcttcgacgc gctccacacc gagcgcccgt acaaggcggc cctgccgcgc
      781 tccgaggcgg tgtccctgct gatccgcgag accgacgcgg gctactggga tcccaaggtg
      841 gtcgagacct tcctcgagat cctccgccag tacaagtagg cctagacgaa gcgggcgagc
      901 atcaagaggc cgagggcgat gcggtaccag ccgaacggca cgaagctgtg ggtctggatc
      961 cagcgcagca gccagcgcac caccacgaag gcggtcagcg ccgacaccac cgtgccgagg
     1021 gccagcatgc cccacttggt gggcggcccg gccgggtggc gaagcgcgtg caaggtctcg
     1081 aacccgcccg cggcgatcag cgtcggaacc cccaccagga acgagaactc ggtggcggcc
     1141 ggccgcgcga ggccgagcag catcgcgatc atgatggtcg ccgccgcgcg cgaggtgccg
     1201 gggaacaccg cagcgagcag ctgagccagc cccaccgcga ccgcgaccgc ccaggtgacc
     1261 tcggaccgcg cggaccggcc gcgcagtccc cactccacca gcaggatcac caccccgccg
     1321 atcagggtcg cccacgcgat cggcgcgacc tccttcggca gcacgaagcc gaggcgcttg
     1381 acggccagcc cgcccgcccc ggtgatcacg aaggcgagca ggagcttcgc caggtagccg
     1441 cccgccgccg gctcgcggac gcgcgcgagg agatcacgca gccggccgcc gaagaccgcg
     1501 agcaccgcga gggcggcgcc ggactggatc gtgatgttga agaggtccga ctggcccggc
     1561 agcagatgcg agttctcgac caggagcagg tgtccggtcg aggagatggg caggaactcg
     1621 gtgacgccct cgatcacgcc cagcaggatg acggccagcc actcgggcat gggagcggtc
     1681 agcccacgat ctcttcgccg ccgtcgacgc cgatcacgtt gccggtgatc cagtacgtgt
     1741 cgggatggct cagcaccacg atggcgcgcg cgacgtcctc ggtggtggtc agccggcgat
     1801 gcgggttgcg ccgggccgcc tgctgggaga actcctcgta gttggggatc ttctgcgccg
     1861 ccggcgtcgc ggtcactccg gcccggatcg agttggcgct gatgccgtgc ggggccagct
     1921 ccgccgcgag ctggcggatg ttggactcca gcgcggcctt cgccgcggac accggcccgt
     1981 agaaggggag cacccgcgcg ccgccggagg aggtcatcgc gtagatccgg ccgccgcgtc
     2041 ccatcaggcc gcggcccacc agctcctggg cccagtacac gaggctgtgg gccatcacgt
     2101 cgagggtcat gtccatctgg gccttcgtca ccgcctcctt gatcggatcg gcgacgaaga
     2161 gcttgagggt gccgaaggcg agcgagtgga gcagcacccg cacctggccc acctcgtcgc
     2221 gctcccgcag cacgcgctcc atctcggtcg ccacctcggc ccgcttctcc tcgtcggcgg
     2281 cgttgacgtt gaagaagcgc gcctgccgcc ccagcgcggt gatctcggcg gcgagccgct
     2341 ccacgttggg cagggtggac ttgcgatcga ggtgcacccc gaacacgttc atcccggccc
     2401 gcgcgagggc caggctggtg gcggcgccga aaccggacga cgcgcccagc acgagggccc
     2461 agccgcgcag cgtgatcgga gaggcgtccg tgctcattcc cttcctcgtg tgagaggttt
     2521 caccggctcg agccgggccc attctagcag aagtcctgct gggggacctc gacctccggc
     2581 atccatttcg cgcacgcccc gggacctttc cgggacatca tggctcgacc gacttcgcta
     2641 ccaggaggac ccgacgatgc ggaatgtccc gactccggtc ctgttcgtgc tcgcgatcgc
     2701 ggcgctggcc ggctgcgccg cgagccccac cgtgcagctg agctcgctcg gccacgaggt
     2761 cgacgtccat ccgcttcggg ggcaatccgt cgagctgctc aagcaggacg acgcggcctg
     2821 cgaatcctgg acgaggagca cgaaggggcc gaatgagccg ttgcccgccg ccgagctccg
     2881 gtacagcgcc tgcgccctga gccgcggcta cagcgtcaag ccctgggacc atcggatcag
     2941 cccgcccgcg agccccactc cggacctcgc gggcgtggtg gccgacctgc aggcctgcgg
     3001 gctcaagcgc gggctgtacg acccgggcgg gctggtcctg ctggggatgg tcgaggccgg
     3061 catgaccgag gcgcgacgag cggaggcgtg ggggtgcctc aagcagcgcg gctatgcggt
     3121 gagctaccaa cccgacgccc gcgccgagtc catccggaag aagtgaaggg ccgccgacaa
     3181 cggcgcccga tagccacgac gagccggggg cgcccccgga gtcccggcgg gagtccgtgc
     3241 tacgatgtgg accgcctatg gcgaagacgg
//
LOCUS       DATMCV010000235         3633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_479303, whole genome shotgun sequence.
ACCESSION   DATMCV010000235 DATMCV010000000
VERSION     DATMCV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479303"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VNU03_05505"
     CDS             <1..192
                     /locus_tag="VNU03_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013880959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HWP73946.1"
                     /translation="YKAVRTERAKLIHWVNRDGVDELYDLAADPYELANLIDDPARAR
                     EREDLRRELARLVVEALGL"
     gene            complement(199..996)
                     /locus_tag="VNU03_05510"
     CDS             complement(199..996)
                     /locus_tag="VNU03_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HWP73947.1"
                     /translation="MTTTDKYDWDAIVNGLNQYLRLRSIPIGMKLFETVEEMEAIPKI
                     RRPKAKHTTDQIVAQARQLGWTVGITMADLVGAQCGAVIGLSPQDDEWLSGKRMAGVW
                     FKTQDDASLHQHAMDCVPYGRYKAMAVAPLTSGRLEPPDICLIYGTPGQMIFFINGLQ
                     WIGYKKMSFTSVGESACADSWGKALKTGEPALTIPCYAERRYGGVADDEMLMAIPPRF
                     LPKVIEGLAALSKNGLRYPIPPFGIQSDAGAGLAVSYPSPATGAGGR"
     gene            complement(993..1559)
                     /locus_tag="VNU03_05515"
     CDS             complement(993..1559)
                     /locus_tag="VNU03_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP73948.1"
                     /translation="MPTMDEYLTRPLGVRLERLRSTPAELARRIEGCDAAALARRPDA
                     GGWSAGEIVCHLRDVEELFLVRFQTILAADDPQILTLGASPEALAAWGIGGEIGHPLD
                     PDRWADDRQYERQDAIRALQAFGRRRGEVVTLLDALSPTQWQRGGIHQARGRMSLGEW
                     VASLAGHDDNHLDQLGQVLGHVPGRRTP"
     gene            complement(1563..2066)
                     /locus_tag="VNU03_05520"
     CDS             complement(1563..2066)
                     /locus_tag="VNU03_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73949.1"
                     /translation="MFGEKHDPEFLKHLFETTEILRKPVAGIIAGYHVLPYILVGPQE
                     AQPASSVEIRGKIKVSPRLVLAPGRAGQTYGDLFKEHELMDEALVARVFSFAYSNRHQ
                     VTLESEDLKIQRWDRNPQAQVERALDELSQREVINTGVILAPNVRFYPVSLDRFITEI
                     LDQEFKP"
     gene            complement(2073..2630)
                     /locus_tag="VNU03_05525"
     CDS             complement(2073..2630)
                     /locus_tag="VNU03_05525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP73950.1"
                     /translation="MTKTATRMLRLMDRAVTLWRIPVIGRAKGRVLRRLLLRHQPRRG
                     IEVGSLFGYSAILVAGHMPRGGRLVCVEENAYLAKFVTSNVEKAGLARQVRVVVGDAL
                     RVLPLLRGPVDFLLIDANKEDYLDYLRAVESRLVKGALVVADNTGIYRRDVKPYLDHV
                     RNDSRYESREYDFDFDCMEVSVFRG"
     gene            complement(2627..3589)
                     /locus_tag="VNU03_05530"
     CDS             complement(2627..3589)
                     /locus_tag="VNU03_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HWP73951.1"
                     /translation="MKLPRRVTICEVGTRDGFQIEPDFIPTDRKIDVVNRLAAAGMPR
                     IEVTSFVSPKAIPQLRDAAEVMAAIVRRPGTVYSALVPNDKGAVRAVDARVDEIHTVV
                     SASESHNLANVNMTIAESLTKLAAVAEVARRAAVPVVSGISCSFGCPFEGDVPVDHLE
                     SVVARLVDVGARGIGLADTTGMANPALVGRVLERLVPRFPGVEWTLHTHDTRAMAIPN
                     IMAAMEHGVAHFDASIGGLGGCPFAPGASGNVCTEDLIHCLHAMGVETGVDLDALIAV
                     SKRVQEIVGRALPGQLVKAGKWDRRYPLPDGVRERIPARTLARP"
BASE COUNT          524 a         1242 c         1307 g          560 t
ORIGIN      
        1 tacaaggcgg tgcggaccga gcgggccaag ctgatccact gggtcaaccg cgacggggtg
       61 gacgagctct acgacctcgc ggccgatccc tatgagctcg cgaacctcat cgacgatccg
      121 gcccgggcga gggagcgcga ggacctgcgg cgagagctcg cccggctcgt ggtcgaggcg
      181 ctcgggctct gagccgcgtc agcggccgcc cgccccggtg gccggactcg ggtagctgac
      241 cgcgagcccg gctcccgcgt cggactggat gccgaagggc gggatcgggt agcggagccc
      301 gttcttgctc agcgcggcca gaccctcgat gaccttgggc aggaagcgcg gcgggatcgc
      361 catcagcatc tcgtcgtcgg ccactccgcc gtagcggcgc tccgcgtagc aggggatggt
      421 gagcgcgggc tcgccggtct tgagcgcctt gccccacgag tccgcgcagg ccgactcgcc
      481 caccgaggtg aacgacatct tcttgtagcc gatccactgg agcccgttga tgaagaagat
      541 catctggccg ggggtgccgt agatcaggca gatgtcgggc ggctcgagac ggccggaggt
      601 gagcggggcc accgccatcg ccttgtagcg cccgtagggc acgcagtcca tcgcgtgctg
      661 gtggaggctc gcgtcgtcct gggtcttgaa ccagaccccg gccatgcgct tgccggagag
      721 ccactcgtcg tcctgcggcg agagcccgat caccgcgccg cactgggcgc ccaccaggtc
      781 cgccatcgtg atgcccaccg tccagccgag ctggcgggcc tgggccacga tctggtcggt
      841 ggtgtgcttg gccttgggac ggcggatctt cgggatcgcc tccatctcct ccacggtctc
      901 gaagagcttc atgccgatcg ggatcgagcg caggcgcagg tactggttga ggccgttcac
      961 gatcgcgtcc cagtcgtact tgtcggtggt ggtcatggcg tcctccgtcc cgggacatgc
     1021 ccgaggacct ggccgagctg atcgagatga ttgtcgtcgt ggccggcgag gctcgccacc
     1081 cactcgccca ggctcatgcg cccgcgcgcc tggtggatgc cgccgcgctg ccattgcgtc
     1141 ggggagagcg cgtcgagcag ggtgacgacc tcgccgcgcc ggcgaccgaa ggcctggagc
     1201 gcccggatcg cgtcctggcg ctcgtactgc cggtcgtcgg cccagcgatc cggatcgagc
     1261 gggtggccga tctccccgcc gatcccccac gccgcgaggg cctccgggga ggcgccgagg
     1321 gtgaggatct gcgggtcatc cgccgcgagg atggtctgga agcggacgag gaacagctcc
     1381 tcgacgtcgc gcaggtggca gacgatctcc cccgccgacc acccgcccgc gtcgggccgg
     1441 cgagcgaggg ccgccgcgtc gcagccctcg atccggcgcg ccagctccgc cggcgtgctc
     1501 cggaggcgct cgaggcggac gccgagcggg cgggtgaggt actcgtccat cgtgggcatg
     1561 ggctacggct tgaactcctg gtcgaggatc tcggtgatga agcgatcgag cgagaccggg
     1621 tagaagcgga cgttgggggc caggatcact ccggtgttga tcacctcgcg ctgggagagc
     1681 tcgtcgaggg cgcgctccac ctgggcctgc gggttgcggt cccagcgctg gatcttgagg
     1741 tcctcgctct cgagcgtcac ctggtgacgg ttcgagtagg cgaaggagaa gacccgggcc
     1801 accagggcct cgtccatcag ctcgtgctcc ttgaagagat cgccgtaggt ctgtccggcg
     1861 cgccccgggg ccagcacgag gcgcggcgag accttgatct tgccgcggat ctccaccgac
     1921 gaggccggct gcgcctcctg cgggccgacc aggatgtagg gcagcacgtg gtagcccgcg
     1981 atgatgcccg cgaccggctt gcgcaggatc tcggtggtct cgaagaggtg cttgaggaac
     2041 tccgggtcgt gcttctcgcc gaacatgccc gcctagccgc ggaagaccga gacttccatg
     2101 cagtcgaagt cgaagtcgta ctcgcggctc tcgtaccgcg aatcgttccg cacgtggtcg
     2161 aggtagggct tcacgtcgcg ccggtagatg ccggtgttgt cggcgaccac gagggctccc
     2221 ttgaccaggc gcgactccac cgcgcgcagg tagtcgaggt agtcctcctt gttggcgtcg
     2281 atcagcagga agtcgaccgg gccgcgcagc agcggcagga ctcggagggc gtcgcccacc
     2341 accacccgga cctgccgggc gaggcccgcc ttctcgacgt tgctcgtcac gaacttggcc
     2401 aggtaggcat tctcctccac gcagaccagg cgtccgcccc gcggcatgtg gccggccacc
     2461 aggatcgccg agtagccgaa gagggagccg acctcgatgc cgcgccgcgg ctggtggcgg
     2521 agcagcaggc gtcgcagcac ccggcccttc gcccggccga tgaccggaat gcgccagagg
     2581 gtgactgcac ggtccatcaa ccggagcatt cgcgtggcgg tcttggtcat gggcgcgcga
     2641 gggtgcgggc cgggatccgc tcgcgcacgc cgtcgggcaa gggatagcgc cggtcccact
     2701 tgccggcctt cacgagctgt ccgggcaggg cgcggcccac gatctcctgc acccgcttcg
     2761 agaccgcgat cagcgcgtcg agatccaccc cggtctccac gcccatggcg tggagacagt
     2821 ggatcaggtc ctcggtgcac acgttgccgg aggcgccggg cgcgaagggg cagccaccga
     2881 gtcctccgat ggaggcgtcg aagtgggcca ccccgtgctc catcgcggcc atgatgttgg
     2941 gaatggccat cgcgcgggtg tcgtgggtgt ggagcgtcca ctcgacgccc gggaagcggg
     3001 gcaccagccg ctcgagcacg cgaccgacca gggccgggtt ggccatgccg gtggtgtcgg
     3061 cgaggccgat gccgcgggcg cccacgtcga cgagccgcgc caccaccgac tcgaggtggt
     3121 cgaccggcac gtcgccctcg aaggggcagc cgaacgagca ggagatgccg gagaccaccg
     3181 gcaccgccgc gcgccgcgcg acctcggcca ccgcggcgag cttggtgagc gactccgcga
     3241 tggtcatgtt cacgttggcc aggttgtggc tctcgctcgc cgagaccacg gtatggatct
     3301 cgtccacccg cgcgtcgacc gcgcgcaccg cccccttgtc gttgggcacc agcgcggagt
     3361 agacggtgcc cgggcgccgc acgatggcgg ccatgacctc ggccgcgtcg cgcagctgcg
     3421 ggatcgcctt gggggacacg aacgaggtga cctcgatgcg cggcatgccg gccgcggcca
     3481 gtcggttcac cacgtcgatc tttcgatcgg tggggatgaa gtcgggctcg atctggaacc
     3541 cgtcccgcgt tcccacctcg cagatcgtca cgcgccgggg cagcttcatg gtttcttctc
     3601 ctgggtcgct tcccactcgt ctctcaggat ctc
//
LOCUS       DATMCV010000236         3726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_414543, whole genome shotgun sequence.
ACCESSION   DATMCV010000236 DATMCV010000000
VERSION     DATMCV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414543"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VNU03_05535"
     CDS             <1..666
                     /locus_tag="VNU03_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP73952.1"
                     /translation="LKADLDARRLAVFRGRWDLVQVLRTHRPEHKALEGRSLADLARE
                     TGKHPVDAWLDLVTADGLDVEFLAGLLNTDEQVVGDLIVHPLTLITLSDAGAHLSLMC
                     DAGYSSTLLGKWVRGLRRLPLEAAVKRLTDDPARAYRIPGRGRLEPGYWADVAIFDPA
                     TVDALPAEWVSDLPAGEPRFVSHARGVEWSLVNGRPVLERGRVVEQPAGARPGRVLRR
                     FDA"
     gene            complement(692..1906)
                     /locus_tag="VNU03_05540"
     CDS             complement(692..1906)
                     /locus_tag="VNU03_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73953.1"
                     /translation="MSDPSRSLLRHPPFALLSSSRVLASVGFQMVGVAVAWQLYELTG
                     SALDLGLVGLVQFVPMILLTLVVGQVADRYDRRVIVVVCEVVKAATAAALALGALGGW
                     QTRTTIFALIALLAAAQAFENPAMSALVPEVVDRRLIAPAMAWVVSAGQTAQVVGPAL
                     GGLLYAFGPAAAYLTAGALFVLAGGLTVAIRIARTVRPREPITVETVFSGVVFIASRR
                     VLLGGMSLDLFAVLFGGATALLPVYAKDILGTGPVGLGLLRSAPAVGALATSIFLARF
                     PLERRIGPTLFWSVMVFGAATVVFGLSTSFALSLAALTVLGAADVVSMVIRLSLAQIR
                     TPDAMRGRVSAVHSLFTGTSNQLGAFESGVAAALLGTVPAVLLGGIGTIAVAALWMLL
                     FPELRRLRRFEE"
     gene            complement(1918..3162)
                     /locus_tag="VNU03_05545"
     CDS             complement(1918..3162)
                     /locus_tag="VNU03_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HWP73954.1"
                     /translation="MSTSELFGIDAPRFWSTVMRSGEIGPGKAGGLRRLALTDADKEM
                     RDLFITWCTEAGCTVTIDRVGNIFARRPGVDDHLPPVLMGSHLDTQFAGGKYDGIVGV
                     LAGLEVLRTLDDRRVRTKRPLELVCWTNEEGARFTPPMVASGAFAGVFDVGWVLDLRD
                     DDGKRFGTELERIGYQGKEAVGGRALDAYFELHIEQGPILDREAVPVGVVVGGYAARG
                     MHVDVHGETAHSGPTPMDKRRNALVGAAMLAVAVNDVGWKYHATEGKATVARLVAWPN
                     KAGILAEYAQLTCDVRHADRAVADRMWTEVKAAMPDCARRANVEMRIAGEWQFGSERF
                     DPGCVRLIREAAQALDVPHRDILSQAGHDAYYVSRVAPTAMIFTPCRDGISHNEAEHA
                     ELDYTSPGVNVLLHAVLARANR"
     gene            complement(3238..>3726)
                     /locus_tag="VNU03_05550"
     CDS             complement(3238..>3726)
                     /locus_tag="VNU03_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWP73955.1"
                     /translation="TCDPAVSLAGANRALRDVEAAMAAILAMPFVDSSRVVIGGVSRG
                     GILSVAYAGQHPEQIKGVINFVGGWLGTGCSSAPTVNRALFSRGGRYPYDSIWIYGDG
                     DPFYPVAHSRDNFGAFQTAGGRGAFHEFVGGHQVSSHPDLWGHAVESYLRQRGLPAEP
                     SP"
BASE COUNT          564 a         1363 c         1329 g          470 t
ORIGIN      
        1 ctcaaggccg atctcgacgc gcgccggctc gcggtcttcc gcggccgctg ggacctggtc
       61 caggtgctgc gcacccaccg gccggagcac aaggccctcg agggccggag cctggccgac
      121 ctcgcacgcg agaccggcaa gcacccggtg gacgcctggc tcgacctcgt gaccgccgac
      181 ggcctcgacg tggagttcct ggccggcctg ctcaacaccg acgagcaggt ggtcggcgat
      241 ctgatcgtcc acccgctcac cctgatcacg ctctccgatg cgggcgcgca tctctcgctg
      301 atgtgcgacg cgggctactc gagcacgctg ctcggcaagt gggtgcgcgg cctccggcgc
      361 ctgccgctcg aggccgcggt caagcggctc accgacgatc ccgcgcgggc ctaccgcatc
      421 cccgggcgtg gacgactcga gcccggctac tgggccgacg tggcgatctt cgacccggcc
      481 accgtggacg cgctgcccgc cgagtgggtg agcgacctgc cggcgggcga gccgcgcttc
      541 gtctcccacg cgcgcggcgt ggagtggagc ctggtcaacg gccggccggt gctcgagcgc
      601 ggccgcgtgg tcgagcagcc ggccggggcg cgcccgggcc gggtgctgcg ccgcttcgac
      661 gcctgaggcg gctgctgaaa gagtcgccgg cctactcctc gaagcggcgg agccggcgga
      721 gctcggggaa gagcagcatc cagagcgcgg ccaccgcgat ggtgccgatg ccgcccagca
      781 gcaccgcggg cacggtgccc agcagggccg cggccacccc ggactcgaac gccccgagct
      841 ggttcgaggt gccggtgaag agcgagtgga ccgcgctcac ccgcccgcgc atcgcgtccg
      901 gcgtgcggat ctgggcgagc gacaggcgga tgaccatgct gaccacgtcg gcggctccga
      961 gcacggtgag cgcggccagc gagagggcga agctcgtgga gaggccgaag accacggtgg
     1021 ccgccccgaa caccatcacc gaccagaaga gggtcggccc gatgcggcgc tcgagcggga
     1081 agcgcgccag gaagatcgag gtcgcgagcg cccccaccgc gggggccgag cgcagcagcc
     1141 cgagcccgac cggcccggtg cccaggatgt ccttggcgta gaccggcagc agcgcggtgg
     1201 cccccccgaa gagcaccgcg aagagatcga gcgacatgcc gcccaggagc acccgccggc
     1261 tcgcgatgaa caccaccccg gagaacacgg tctcgaccgt gatcggctcg cgcggacgga
     1321 cggtgcgcgc gatgcggatc gccaccgtga gcccgccggc caggacgaac agggcccccg
     1381 cggtgaggta ggccgcggcc gggccgaacg catagagcag cccaccgagc gcggggccga
     1441 cgacctgggc ggtctgcccg gcggagacga cccacgccat cgcgggggcg atcagcctcc
     1501 ggtccaccac ctcgggcacc agcgcgctca tggcggggtt ctcgaatgcc tgggcggcgg
     1561 cgagcagggc gatcagggcg aagatcgtgg tgcgcgtctg ccaaccgccc agcgcgccca
     1621 gggcgagggc cgccgccgtc gccgccttca cgacctcgca gacgacgacg atcacgcggc
     1681 ggtcgtagcg atccgcgacc tgtccgacca cgagggtcag caggatcatc ggcacgaact
     1741 gcacgaggcc gacgaggccc aggtcgagcg cgctcccggt cagctcgtac agctgccagg
     1801 ccaccgcgac ccccaccatc tggaagccca cgctggccag gacccgcgac gaggagagca
     1861 gcgcgaacgg cggatgccgc aggagcgagc gcgacgggtc ggagatctcg cgggcctcta
     1921 ccggttggcg cgggccagca ccgcgtggag gagcacgttc acccccggcg aggtgtagtc
     1981 gagctcggcg tgctcggcct cgttgtgcga gatgccgtcg cggcacgggg tgaagatcat
     2041 cgcggtgggc gcgacccggg agacgtagta ggcgtcgtgc ccggcctggc tcaggatgtc
     2101 gcgatggggc acgtcgagcg cctgggccgc ctcccggatc aggcgcacgc agccgggatc
     2161 gaagcgctcg ctgccgaact gccactcgcc cgcgatgcgc atctccacgt tggcccggcg
     2221 ggcgcagtcg ggcatcgcgg ccttgacctc ggtccacatc cgatccgcca ccgcgcggtc
     2281 ggcgtggcgc acgtcgcagg tgagctgggc gtactcggcg aggatgcccg ccttgttggg
     2341 ccacgccacc aggcgcgcca ccgtggcctt gccctcggtc gcgtggtact tccaccccac
     2401 atcgttgacc gccaccgcca gcatcgcggc gcccaccagc gcgttgcgcc gcttgtccat
     2461 cggggtcgga cccgagtgcg cggtctcccc gtgcacgtcg acgtgcatgc cgcgcgccgc
     2521 gtagccgccc accacgacgc cgaccgggac cgcctcacga tcgaggatcg gcccctgctc
     2581 gatgtgcagc tcgaagtagg cgtcgagcgc ccggccgccg accgcctcct tgccctggta
     2641 gccgatgcgc tcgagctcgg tgccgaaccg cttgccgtcg tcgtcgcgca ggtcgagcac
     2701 ccagcccacg tcgaagactc ccgcgaaggc gcccgaggcc accatcgggg gcgtgaagcg
     2761 cgcgccctcc tcgttggtcc agcacaccag ctcgagcggc cgcttggtgc gcacgcggcg
     2821 gtcgtcgagg gtgcgcagca cctcgagccc ggccaggacg cccacgatgc cgtcgtactt
     2881 gccgcccgcg aactgggtgt cgaggtggct gcccatcagc accggcggca ggtggtcgtc
     2941 gacgccggga cgtcgcgcga agatgttgcc cacgcggtcg atcgtcaccg tgcagcccgc
     3001 ctcggtgcac caggtgatga agagatcgcg catctccttg tccgcgtcgg tgagcgccag
     3061 gcggcggaga ccgcccgcct tgccgggccc gatctcgccc gaccgcatga cggtggacca
     3121 gaagcgcggc gcgtcgatgc cgaacaactc gctcgtggac atggctctcg cgccctcccg
     3181 gcgcgctccg gtcttaccct gtcgcgcgcg aagctgtcaa gcgtgtgggg ttccgactca
     3241 cggtgagggc tcggccggca ggccgcgctg cctcaggtaa gactccacgg cgtgtcccca
     3301 cagatcggga tggctcgaca cctgatggcc gccgacgaac tcatggaacg cccctctccc
     3361 accggcggtc tgaaaggcgc cgaaattgtc ccggctgtgc gccacgggat agaaggggtc
     3421 gccgtctccg tagatccaga tcgagtcgta gggatagcgt ccgccgcgcg agaagagagc
     3481 gcgattgacg gtcggggcgg aactgcaacc cgtcccgagc cagccgccga cgaagttgat
     3541 cactcccttg atctgttcgg gatgctgtcc ggcgtatgcc acgctcagga ttccgccgcg
     3601 cgagacaccg ccgatcacca cgcgggagct atccacgaat ggcatcgcga ggatcgcagc
     3661 catcgccgcc tcgacgtcac ggagggcgcg gttcgccccc gccaacgaga ccgcagggtc
     3721 gcatgt
//
LOCUS       DATMCV010000237         5163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_414561, whole genome shotgun sequence.
ACCESSION   DATMCV010000237 DATMCV010000000
VERSION     DATMCV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414561"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="VNU03_05555"
     CDS             complement(<1..1889)
                     /locus_tag="VNU03_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73956.1"
                     /translation="MTRAWLPPTLLAAYAIAFGWRALGGGLLVFDDHPGQLYRVVQAI
                     TVGLAPWRFDPGWWAGYAELQYYPPGFAWLGALLHHAAGGALSAATVYQALLWLAWVL
                     PGAATYVLLRRLLGDPWLALPGALVALTLSAGCRSGVEEGLRWGLVGARLGWGALSLQ
                     ALVLLRWAEGAPRPPLGAAGLVAAVTLLHPAHTSTAVAMVLLAAAWRAPRRRRLVEGL
                     ALVALGLGLAGVWLLPLLAHLDMALPLAWQDRSLPVLGWRVISQPVLPVLALLGLIAW
                     RSSAPGREARGRWLLAMAPLTIGLVLVDALVAEPLGAAWLPADRLVDGLHWALVLGGA
                     LGVAALGARLARPRVVVPLALAACVPLAWGPYEPGLSLWPWAGQWPKEQEVTRGLRLD
                     ALWPALQAAPPGRILFVRSGVSLDWRPEWWRPHTHLTALTPIRTGRAIVGGTFTHPSP
                     VAGLIYTGSAANRPLTLLAEQLDGRTLFGVPLEALGAETFDRLAARLGISTVVAMDED
                     AGVAPFLVSNPALRRTSRIGSFNVFVFRDSREDLTSIGPQRWQVPVSPDASGWAGLAI
                     AFSPLWVARAGGTRIPTRRDALGLLEVMPPAGTTVVELEHRAGAAEWAGVAVSLLSLS
                     ALGLLS"
     gene            2040..3296
                     /gene="thrC"
                     /locus_tag="VNU03_05560"
     CDS             2040..3296
                     /gene="thrC"
                     /locus_tag="VNU03_05560"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWP73957.1"
                     /translation="MDTVKGLKCRECGRGYPASPIHVCEFCFGPLEVDYDYEAIGARV
                     SRARIEAGPPSIWRYADLLPLDVPDGEPPIGPHVGFTPLVRAHNLAREWGVRELYVKN
                     DAVCHPTCSFKDRVVAVAVAKAREFGFDTVACASTGNLANSVAAHAAEAGLKACVFIP
                     ADLEQGKVIGTLVYAPTLVEVQGTYDEVNRLCAEIGDKYHWAFVNINLRPYYAEGSKT
                     MGYEIAEQLGWRAPAHVVVPCAGGSLLTKIAKAFQELARLGLIPQARTRMYAAQAAGC
                     GPIVTMIQKDTDVLEPVRPQTIAKSLAIGNPADGYYAYRAVKDSGGYGEHATDEEIID
                     GMRLLARTEGIFAETAGGVTVAATRKLIEAGRIPRDEPIVMCVTGNGLKTPDVLHDRL
                     GRDVTIQPSLQAFDRALADLTSRTVA"
     gene            3319..3597
                     /locus_tag="VNU03_05565"
     CDS             3319..3597
                     /locus_tag="VNU03_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HWP73958.1"
                     /translation="MAVLVRIPTPLRTLTKGAAEVQADASTVTDLIDVLERQFPGLKE
                     RLLEDGGQLRRFINIYINQEDIRFLQGATTSLKQGDEVSIVPAIAGGR"
     gene            3625..4788
                     /locus_tag="VNU03_05570"
     CDS             3625..4788
                     /locus_tag="VNU03_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWP73959.1"
                     /translation="MISKRVQGFTESVIREMTRVNNQYDGINLAQGMPNFPPPRELVE
                     AAHRAIDGDFHQYAITWGTPRLRQAIAEKYRTFYGMEIEADRHVTVCCGSTETMLATL
                     LAVLNPGDEVIIFEPFYENYGPGCIIAGAEPVFVPLEPPDFTFDPERLARAVTSRTRA
                     VVFNSPNNPSGKVFSRAELQVIADVCLKHDLLAITDEIYEHIVYDGLDHTPIATLPGM
                     ADRTVTISGISKSYSVTGWRVGYAVASPELSLGIRRAHDFITVGAPHPLQEAAVTALG
                     LPDSYYVHLRESYQARRDLLLGKVEEAGFVAFRPHGAYYILTEAEHFLKRFGCKDDHE
                     FALYLVREVGVATVPGSSFYAHGELGRTKIRFCFPKTDDMLIEAGRRLQKLAR"
     gene            4785..>5163
                     /locus_tag="VNU03_05575"
     CDS             4785..>5163
                     /locus_tag="VNU03_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HWP73960.1"
                     /translation="MTLREAARSVFDAGLAAADVAPLVRRALADVRLAPSGRVLVVGA
                     GKASGAMATAAEAALGDAITDGLVVVKDGYRSATRRIRLVEAGHPVPDARGEAAARQI
                     RAMAEAAGADDLLLVLVSGGGSAL"
BASE COUNT          812 a         1918 c         1805 g          628 t
ORIGIN      
        1 gacagcagcc cgagcgcgct caggctcagc aggctgaccg ccacgcccgc ccattccgcc
       61 gcgccggcgc ggtgctcgag ctccaccacc gtggtcccgg ccggcggcat cacctcgagc
      121 aggcccagcg cgtcccggcg cgtcgggatg cgcgtaccgc cggcccgggc cacccagagc
      181 ggggagaagg cgatcgcgag cccggcccac cccgacgcgt ccggcgacac cggtacctgc
      241 cagcgctggg gaccgatcga ggtcagatcc tcccgcgagt cgcggaagac gaagacgtta
      301 aaggagccga tgcgagaggt acgccgaaga gctggattcg acacgagaaa cggcgcgacg
      361 ccagcgtcct cgtccatcgc caccaccgtc gagatcccga ggcgggccgc gagccggtcg
      421 aacgtctccg cgccgagcgc ctcgagcggg acgccgaaga gggtccggcc gtcgagctgc
      481 tcggcgagca gggtgagggg acggttggcc gcggagccgg tgtagatcag gcccgccacc
      541 ggtgacgggt gcgtgaacgt cccgccgacg atcgcgcgcc cggtccggat cggcgtgagc
      601 gcggtgaggt gggtgtgcgg gcgccaccat tcgggccgcc agtcgaggga cacacccgag
      661 cgcacgaaca ggattcggcc cggcggcgcg gcctggagcg cgggccagag ggcgtcgagc
      721 ctgagcccgc gcgtcacctc ctgctccttc ggccattgcc cggcccatgg ccacagcgac
      781 aggccgggct cgtacgggcc ccaggcgagc ggcacacacg cggccagcgc cagcgggacg
      841 accacgcggg gccgggcgag gcgggcgccc agcgcggcca cgccgagcgc gccgccgagc
      901 acgagcgccc agtggagccc gtccaccagc cggtcggcgg gcagccacgc ggctccgagc
      961 ggctccgcga cgagcgcatc gaccagcacc aggccgatcg tcagcggcgc catggcgagc
     1021 agccatcgcc cgcgggcttc gcggcccggc gccgacgagc gccaggcgat gaggccgagc
     1081 agcgcgagca ccgggagcac cggctgcgag atgacacgcc agccgaggac ggggagcgag
     1141 cggtcctgcc acgcgagcgg gagcgccatg tccagatgcg ccagcagcgg cagcagccac
     1201 acaccggcca ggcccagccc gagcgccacg agggcgagcc cctcgaccag ccgccggcgg
     1261 cgcggcgcgc gccacgccgc ggcgagcaag accatcgcca ccgcggtgga agtatgtgcc
     1321 ggatggagga gcgtcaccgc agcgacgaga ccggcggctc cgagcggcgg ccgcggcgca
     1381 ccctccgccc agcgcaggag gacgagggcc tgcagggaga gcgcgcccca gccgagccgg
     1441 gcgccgacga gcccccagcg caggccttcc tccacgccgc tccggcagcc cgctgagagc
     1501 gtgagcgcca cgagcgcgcc gggcagcgcg agccacgggt cgccgaggag ccgacgcagg
     1561 agcacgtagg tggccgcgcc cggaagcacc cacgcgagcc agagcagcgc ctggtacacc
     1621 gtcgcggcgg agagcgcacc gcccgccgcg tgatgcagca gggcgccgag ccacgcgaag
     1681 cccggcgggt agtactgcag ctcggcgtag cccgcccacc atcccggatc gaagcgccac
     1741 ggcgcgagcc cgaccgtgat cgcctggacc acgcggtaga gctgacccgg atggtcgtcg
     1801 aagacgagca ggccaccgcc gagcgcccgc cacccgaagg cgatggcgta ggcggccagc
     1861 agcgtgggcg gcagccaggc gcgggtcacg ggtggcgatg atacggcatc gcgttgacaa
     1921 tccgcgtcgt ggagtgttaa gtaaacggaa ttgttgccgc tagatcggtg gtccggacga
     1981 ggccaacccg gaccgtgcag ctaattccgg ggctcaaatc ccctaccgat ggagaggcca
     2041 tggacaccgt gaagggcttg aagtgccggg agtgtggccg cggctacccc gcctccccga
     2101 ttcacgtctg cgaattctgc ttcggtcccc tcgaggtcga ctacgactac gaggcgatcg
     2161 gcgcgcgggt gagccgcgcg cgcatcgagg ccggtccccc gtcgatctgg cgctacgccg
     2221 atctcctccc gctcgacgtg cccgacggcg agccgccgat cgggccccac gtgggcttca
     2281 cgccgctggt ccgcgcgcac aacctggccc gagagtgggg cgtgcgcgag ctctacgtga
     2341 agaacgacgc ggtctgccat ccgacctgct ccttcaagga ccgggtggtg gcggtcgcgg
     2401 tggccaaggc gcgggagttc ggcttcgaca cggtggcgtg cgcctcgacc ggcaacctcg
     2461 ccaactcggt ggccgcccac gcggcggaag cgggtctcaa ggcctgcgtc ttcatccccg
     2521 ccgatctgga gcagggcaag gtgatcggga cgctcgtcta cgccccgacc ctggtcgagg
     2581 tgcaggggac ctacgacgag gtcaaccggc tctgcgccga gatcggcgac aagtaccact
     2641 gggccttcgt caacatcaac ctccggccct actacgccga gggctcgaag acgatgggct
     2701 acgagatcgc cgagcagctc ggctggcggg cgcccgccca cgtggtggtg ccctgcgcgg
     2761 gcggctcgct gctcaccaag atcgccaagg ccttccagga gctggcgcgc ctcgggctca
     2821 tcccccaggc ccgcacccgc atgtacgcgg cgcaggccgc gggctgcggc cccatcgtca
     2881 ccatgatcca gaaggacacc gacgtgctcg agccggtgcg gccgcagacc atcgccaagt
     2941 ccctcgccat cggcaacccg gccgacggct actacgccta ccgcgcggtc aaggactcgg
     3001 gcggctatgg cgagcacgcc accgacgagg agatcatcga cgggatgcgg ctgctcgccc
     3061 gcaccgaggg gatcttcgcg gagaccgccg gcggggtgac ggtggcggcc acccgcaagc
     3121 tgatcgaggc gggccgcatc ccgcgtgacg agccgatcgt gatgtgcgtc accggcaacg
     3181 ggctcaagac gcccgacgtg ctgcacgatc gactcggccg cgacgtcacc atccagccat
     3241 cgctccaggc cttcgaccgg gcgctggccg acctcacatc cagaaccgtg gcgtaggcgc
     3301 cgcgtcgagg agatccccat ggccgttctc gtccgcattc cgaccccgct gcgcaccctc
     3361 accaagggcg cggccgaggt ccaggccgac gcttccaccg tcaccgacct gatcgacgtg
     3421 ctcgagcgcc agttccccgg cctcaaggag cgcctgctcg aggacggcgg gcagctgcgc
     3481 cgcttcatca acatctacat caatcaggaa gacatccggt tcctccaggg cgccaccacg
     3541 tcgctcaagc agggcgacga ggtctccatc gtcccggcca tcgcgggcgg ccgctagccg
     3601 cccccggcac gcctccgtcg ggccatgatc tcgaagcggg tccagggctt caccgagtcg
     3661 gtgatccggg agatgacccg ggtcaacaac cagtacgacg gcatcaatct ggcccagggc
     3721 atgccgaact tcccgccgcc gcgcgagctg gtggaggcgg ctcaccgggc catcgacggc
     3781 gacttccacc agtacgcgat cacctggggc accccgcgcc tccggcaggc gatcgccgag
     3841 aagtaccgga cgttctacgg catggagatc gaggccgacc gccacgtcac ggtctgctgc
     3901 ggctccaccg agaccatgct ggccaccctg ctcgcggtgc tcaatccggg cgacgaggtg
     3961 atcatcttcg agcccttcta cgagaactac gggcccgggt gcatcatcgc gggggccgag
     4021 ccggtcttcg tgccgctgga gccgcccgac ttcacgttcg acccggagcg cctcgcccgc
     4081 gcggtcacct cgcggacccg cgcggtcgtg ttcaacagcc cgaacaatcc ctccggcaag
     4141 gtcttctccc gggccgagct acaggtgatc gccgatgtct gcctcaagca cgatctcctg
     4201 gccatcaccg acgagatcta cgagcacatc gtctacgacg gcctcgacca caccccgatc
     4261 gccacgctgc ccggcatggc cgaccgcacc gtgacgatct ccggcatctc caagtcctac
     4321 tcggtgaccg gctggcgggt cggctacgcg gtggcgagcc ccgagctgtc gctcggcatc
     4381 cggcgcgccc acgacttcat caccgtcgga gcgccgcacc cgctccagga agccgcggtg
     4441 accgcgctcg gcctgcccga ctcgtactac gtgcatctgc gcgagagcta ccaggcccgc
     4501 cgcgatctcc tgctgggcaa ggtggaggag gcgggcttcg tcgccttcag gccccacggt
     4561 gcgtactaca tcctcaccga ggccgagcac ttcctgaagc gcttcggctg caaggacgac
     4621 cacgagttcg ccctttacct ggtgcgggag gtcggggtcg ccaccgtgcc cggctcgtcc
     4681 ttctacgccc acggcgagct cgggcggacc aagatccgct tctgcttccc caagaccgac
     4741 gacatgctga tcgaggcggg gcggcggctg cagaagctcg cccgttgacc ctccgcgagg
     4801 cggcccgctc ggtcttcgac gccgggctcg cggccgccga cgtcgctccg ctggtccgcc
     4861 gcgccctcgc cgacgtgcgt ctcgcgccgt ccggccgcgt gctcgtggtg ggcgcgggca
     4921 aggcctcggg ggcgatggcc accgcggccg aggccgcgct gggcgacgcg atcacggacg
     4981 ggctggtcgt ggtgaaggac ggttaccgga gcgccacccg ccgcatccgt ctcgtggagg
     5041 ccggccaccc ggtgcccgac gcgcgcggag aggcggcggc ccgtcagatc cgcgcgatgg
     5101 cggaggcggc cggggcggac gacctgctcc tcgtcctggt ctcgggcggc ggctcggccc
     5161 tga
//
LOCUS       DATMCV010000238         3971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_414640, whole genome shotgun sequence.
ACCESSION   DATMCV010000238 DATMCV010000000
VERSION     DATMCV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414640"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VNU03_05580"
     CDS             <1..678
                     /locus_tag="VNU03_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HWP73961.1"
                     /translation="FQGMDAAGKDGAIKHVMSGVNPQGCDVRSFKAPSPEELDHDFLW
                     RAHKAAPERGRIGIFNRSYYEEVLVVRVHRELLAAERLPEPLVGKHIWDERYEDINAH
                     ERYLTRNGVVIRKFFLHLSKEEQRKRFLARLDDPAKNWKFSLGDIQERKFWSDYQDAY
                     EEMVRQTATKHAPWYVVPADHKWYTRLVVAAAIVDTLAGLDLAFPEEDRAKRKELEKV
                     REALLKS"
     gene            complement(668..1558)
                     /locus_tag="VNU03_05585"
     CDS             complement(668..1558)
                     /locus_tag="VNU03_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP73962.1"
                     /translation="MTAPSPLSPQGRGQGEGLQGEGLQGEGLQGEGRQGEGRTVSVWQ
                     GRVPFRVHVKGAGPAVMFLHGPWGLTWGPFLDALAQQFTVYAPEHPGTSPGEPEAIQQ
                     VDTLWDLVLCYDELLEALKLDGLMLVGHSFGAMMACEVAALRPARVRRLALLDPIGLW
                     LEEAPVVNWMLLGPAEMAAHVFDKPDGAAARAMFAVPEDPEEGALARTRLTWAMGSTG
                     KFIWPIPDKGLKKRIHRVGAPALLVWGEADRLVPRAYAKEFAGRLHDARIEVVPGAGH
                     APHLEQPEATARVVAAFLKT"
     gene            complement(1555..2793)
                     /locus_tag="VNU03_05590"
     CDS             complement(1555..2793)
                     /locus_tag="VNU03_05590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP73963.1"
                     /translation="MKVSAFHLMPHRELPPDFERRYPSVWVTPPWGELADARRVGQYY
                     NWTLDELLFAARAGFDGVCTNEHHQNAYGFMPSPNLMGSVLAKQTNGLDVAIVQMGMT
                     LPTTSPPIRVAEEYGMLDCISGGRLVAGLPLGSPMDVNLAYGITPMEHRERYREAFEL
                     VLKAWQAKEIFAWNGKYFQLGQVNLWPRPIQEPHPPVWVPGSGSISTFDFATEHNVCY
                     CFLSYAGARAASTMMDGYWDVVDKKGLERNPYRAGFLQLVAVSETDAQAEIDYGKHVE
                     YFYHKCLHVPPQWFAPPGNQDYRSLLAANRNPVRRAEDPKTLRYKDFVNKGYVIAGSP
                     ATVRERLVEEVIKRLHVGNLMLLIQIGSMPHELTLKNTELLAREVLPGLRDIWDDKGW
                     ENHWWPKSLRGARQPAAAAR"
     gene            2978..>3971
                     /locus_tag="VNU03_05595"
     CDS             2978..>3971
                     /locus_tag="VNU03_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73964.1"
                     /translation="MTAWRNLAAYPPGHPARASALATAHTRLRASLAAASPLVLGISR
                     DALVSGGKKIEASHVRAFARALYRRNGGVLRFEEDVEPRELETFLALLGDTGPARDRP
                     LTVEELQAAGITHIHVAAIDYSALVTTSDLDEPGGDDGSLWDGLLQTLLAGQPVFGGG
                     MTLPRERYSAETIASLFRGGPGAGGSGPGGSESGGSPGPGGGPPGAGTGSGGPIGGGP
                     GGGGPGGSGSAGGGWPGSGAGRGAGAQAPPPAGETGGRGGQGARLGLAAAHTLAAAVA
                     ARLASADDDQRETLAPQIIQLIRALPNEVREPVLAAALRVLATEDGASAHLARLA"
BASE COUNT          560 a         1425 c         1431 g          555 t
ORIGIN      
        1 ttccagggca tggacgcggc gggcaaggac ggcgcgatca agcacgtcat gtccggggtc
       61 aatccgcagg gctgcgacgt gcgctccttc aaggcgccga gccccgagga gctcgaccac
      121 gacttcctct ggcgggccca caaggccgcc ccggagcgcg ggcggatcgg gatcttcaac
      181 cgctcctact acgaggaggt gctggtggtc cgggtccacc gggagctgct cgcggccgag
      241 cgcctgccgg agccgctcgt gggcaagcac atctgggacg agcgctacga ggacatcaac
      301 gcccacgagc gctatctcac ccggaacggg gtggtgatcc gcaagttctt cctgcacctc
      361 tcgaaggagg agcaacgcaa gcgcttcctg gcccggctcg acgatccggc caagaactgg
      421 aagttctcgc tcggcgacat ccaggagcgg aagttctggt ccgactacca ggacgcctac
      481 gaggagatgg tgcgccagac cgcgaccaag cacgccccgt ggtacgtggt gccggccgac
      541 cacaagtggt acacgcgcct ggtggtggcc gcggccatcg tggacacgct cgccgggctc
      601 gacctcgcgt tccccgaaga ggatcgagcg aagcggaagg agctggagaa ggtccgggag
      661 gcgctcctca agtcttgagg aaagcggcca ccacgcgcgc ggtcgcctcc ggctgctcga
      721 ggtgcggcgc gtggccggcc cccggcacga cctcgatgcg cgcgtcgtgg aggcgcccgg
      781 cgaactcctt ggcgtaggcc cgcggcacga ggcgatcggc ctcgccccac actagcaggg
      841 ccggcgcgcc gacgcggtgg atgcgcttct tgaggccctt gtcggggatc ggccagatga
      901 acttgccggt cgatcccatc gcccacgtca ggcgggtgcg cgcgagcgcc ccttcctcgg
      961 gatcctcggg caccgcgaac atcgcgcggg cggcggcgcc gtcgggcttg tcgaacacgt
     1021 gggccgccat ctcggccggc cccagcagca tccagttcac gaccggcgcc tcctcgagcc
     1081 agagcccgat ggggtcgagg agggcgaggc ggcgcacccg cgccgggcgc agcgcggcca
     1141 cctcgcacgc catcatcgcg ccgaaggaat ggccgaccag catcagcccg tcgagcttca
     1201 gcgcctccag cagctcgtcg tagcagagga cgaggtccca gagggtgtcg acctgctgga
     1261 tcgcctccgg ctcgcccggc gaggtgccgg gatgctcggg ggcgtagacc gtgaactgct
     1321 gggcgagggc gtcgaggaac ggcccccacg tgaggcccca cgggccgtgc aggaacatca
     1381 ccgccgggcc cgcgcccttc acgtggaccc ggaagggcac gcgcccctgc cagacgctga
     1441 cggtgcgccc ctcaccctgc cgcccctcac cttgtagccc ctcaccttgt agcccctcac
     1501 cctgtagccc ctcaccctgc cctctcccct ggggggagag ggggctgggg gcggtcatcg
     1561 ggcggcggcg gctggctggc gcgcgccgcg caggctcttg ggccaccagt ggttctccca
     1621 gcccttgtcg tcccagatgt cgcgcaggcc gggcagcacc tcgcgggcga gcagctcggt
     1681 gttcttgagc gtcagctcgt gcggcatcga gccgatctgg atcagcagca tcaggttgcc
     1741 cacgtgcagg cgcttgatga cctcttccac gaggcgctcg cgcaccgtcg ccgggctgcc
     1801 cgcgatgacg tagcccttgt tcacgaagtc cttgtagcgg agcgtcttgg gatcctcggc
     1861 ccgccgcacc ggattgcgat tggccgcgag caggctccgg tagtcctgat tgcccggcgg
     1921 ggcgaaccac tgcggcggca cgtggaggca cttgtggtag aagtactcga cgtgcttgcc
     1981 gtagtcgatc tccgcctgcg cgtcggtctc cgacaccgcg accagctgca ggaaccccgc
     2041 gcgataggga ttgcgctcga gccctttctt gtccaccacg tcccagtagc cgtccatcat
     2101 ggtggaggcc gcgcgcgccc ccgcgtagga gaggaagcag tagcagacgt tgtgctcggt
     2161 cgcgaagtcg aaggtgctga tgctgcccga gccgggcacc cacaccggcg ggtgcggctc
     2221 ctggatgggc cgcggccaga ggttgacctg gccgagctgg aagtacttcc cgttccacgc
     2281 gaagatctcc ttcgcctgcc acgccttcag caccagctcg aacgcctcgc ggtagcgctc
     2341 gcggtgctcc atcggcgtga tgccgtaggc gaggttgacg tccatcgggc tgccgagggg
     2401 gagccccgcc accaggcggc ccccgctgat gcagtcgagc atgccgtact cctcggccac
     2461 ccggatgggc ggcgaggtcg tcggcagcgt catgcccatc tggacgatgg ccacgtcgag
     2521 gccgttggtc tgcttggcca gcacgctgcc catcaggttg gggctcggca tgaagccgta
     2581 ggcgttctgg tgatgctcgt tggtgcagac cccgtcgaag ccggcgcgcg cggcgaacag
     2641 gagctcgtcg agcgtccagt tgtagtactg cccgacccgg cgggcgtcgg ccagctcgcc
     2701 ccagggcggg gtcacccaga cgctcgggta ccggcgctcg aaatccgggg gcagctcacg
     2761 atgcggcatc aggtggaacg cgctgacttt catgaccgag gctctccctt cccgatcgga
     2821 tggtgcgagc ataagcgacg cggcccgggc gctcaagttg cgggaaaagc gcgcggcgtc
     2881 ggctagactg ctgacgggct agacggtcgc ccagaacgcc aacaacgtgg agacacccgg
     2941 ccccccgccc actctgctcg agctgatgtc gtcgctggtg acggcatggc ggaatctggc
     3001 cgcctacccg cccgggcacc cggcgcgggc ctcggccctg gccaccgccc acacccggct
     3061 tcgggcctcc ctggcggccg cgagcccgct cgtcctcggg atctcccgcg acgccctcgt
     3121 gagcggcggc aagaagatcg aggcctcgca cgtgcgtgcg ttcgcccggg cgctctaccg
     3181 ccgcaacggc ggcgtgctgc gcttcgagga ggacgtcgag ccgcgcgagc tggagacctt
     3241 cctcgcgctc ctgggcgaca ccgggcccgc ccgggaccgg ccattgaccg tcgaggagct
     3301 gcaagcggcc ggcatcaccc acatccacgt cgccgccatc gactacagcg cgctcgtcac
     3361 gacctccgac ctggacgagc ccggcggcga cgatgggtcg ctctgggatg ggctcctcca
     3421 gacgctgctc gccggccagc cggtgttcgg tggtgggatg accctccccc gcgagcgcta
     3481 ctcggcggag accatcgcct cgctcttccg cggcggcccg ggcgcaggcg gctccggccc
     3541 gggtggatcc gagagcggcg gctcgcccgg ccccggcggc ggcccgccgg gggccggcac
     3601 ggggagcggt ggtccgatcg gcggagggcc aggaggtggc gggccgggtg ggagcgggtc
     3661 ggccgggggt ggctggcccg gtagcggcgc cgggcgcggc gcgggggccc aggccccccc
     3721 gccggccggc gagaccggtg gccgcggcgg ccagggggcc cggctcggcc tcgccgccgc
     3781 gcacacgctc gccgccgcgg tcgcggcgcg cctcgcgagc gcggacgacg atcagcggga
     3841 gacgctggcg ccgcagatca tccagctgat ccgggccctg cccaacgagg tgcgcgagcc
     3901 ggtgctggcc gccgccctcc gggtcctcgc gaccgaggac ggggcgagcg cgcacctggc
     3961 gaggctcgcg g
//
LOCUS       DATMCV010000239         2879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_346449, whole genome shotgun sequence.
ACCESSION   DATMCV010000239 DATMCV010000000
VERSION     DATMCV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346449"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VNU03_05600"
     CDS             complement(<1..991)
                     /locus_tag="VNU03_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWP73965.1"
                     /translation="MKITTVESLHADGGWRSLDFLKISTDEGLVGWSEYNESFGGVGV
                     SAAIAGLAPLLIGKDPRPWEAHVALLSAVRRQAAGGVIQQAIGAIENALLDIKARSLG
                     VPVYSLFGGPVRDRIRLYWSHCATYRVSRAKEMSIPPVRTLDDIVAMGREVVAKGYTA
                     LKTNALLLGDNPRAHVPGFGRSAGFPELNADRHVLDAIRAELAAFREGAGPGMDILVD
                     LNFNYKTEGFLKMARAMEPYDLFWVEMDTRDAKALAYVRSRTTIPVASCECLFGRRDY
                     RPFFEQQAVDVAIIDTPWNGVGESLKIAAMADTYEVNVAPHNFYGHLATMMNAH"
     gene            complement(1005..1832)
                     /locus_tag="VNU03_05605"
     CDS             complement(1005..1832)
                     /locus_tag="VNU03_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWP73966.1"
                     /translation="MRRGRHPWLSTAGFYALASLFMLPTVFVFYWMITLSLKPQVEAM
                     AYPPAFVRFSVTAAGYREVFTKYPFFLYTWNSLVVAAGSTALGLVVGLPAAYSIARWR
                     QQRLAVVILVARIIPGIAYLIPWYIFFRQLRMVDTYGALILTHLIVSLPIIIWVMISF
                     FEDVPAEMEDAALIDGCSYWSAFWRIALPLVKPGVVATAILSFVFSWNNFLFSVILAG
                     RATRTLPIAVYTMISYEEINWGTLAAAATLITLPVLLVALVAQRHIVSGLTFGAVKQ"
     gene            complement(1829..2653)
                     /locus_tag="VNU03_05610"
     CDS             complement(1829..2653)
                     /locus_tag="VNU03_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWP73967.1"
                     /translation="MLLVAVIIVYPIAYTVWMSLQEWFASSLTAPHFIGLANYRKIAL
                     ADARFQEAVGRTVYFTIVAVGAETILGLAMALLFNRQFWGRGLLRTLAILPMVATPTA
                     IGLVFVMMYHPSLGVANYLLSVVGLSPWRWTYSSQTALYALALVDVWQWTPLIMLIAL
                     AGLASLPREPYEAAHIDGASTLRAFWHITLPLLRPALVVAVLFRAIDALKTFDIIFVM
                     TQGGPANASETINILLFNQAFSYFNMGYASSMAVALFAVVMGASLILIKVRRTTTW"
BASE COUNT          515 a          906 c         1040 g          418 t
ORIGIN      
        1 agtgggcgtt catcatggtc gcgaggtggc cgtagaagtt gtgcggggcc acgttgacct
       61 cgtaggtgtc ggccatcgcc gcgatcttga gcgattcgcc caccccgttc cacggcgtgt
      121 cgatgatggc cacgtccacc gcctgctgct cgaagaacgg ccggtagtcg cgccggccga
      181 agaggcactc gcacgaggcg accgggatcg tggtgcggct ccgcacgtag gcgagcgcct
      241 tcgcgtcgcg ggtgtccatc tcgacccaga agagatcgta gggctccatg gcccgcgcca
      301 tcttgaggaa gccctcggtc ttgtagttga agttgaggtc caccaggatg tccatgccgg
      361 gccccgcgcc ctcgcggaag gcggcgagct cggcgcggat ggcgtcgagc acgtgccggt
      421 cggcgttgag ctcggggaag ccggcgctgc ggccgaaccc agggacgtgc gcgcgcggat
      481 tgtcgcccag caggagcgcg ttggtcttca gcgcggtata gcccttggcc accacctcgc
      541 gtcccatcgc cacgatgtcg tcgagcgtgc gcaccggcgg gatgctcatc tccttggcgc
      601 ggctcacgcg gtaggtggcg cagtgcgacc agtacaggcg gatgcggtca cgcaccgggc
      661 cgccgaagag ggaatagacc ggcacgccga gggagcgggc cttgatgtcg agcagcgcgt
      721 tctcgatggc cccgatggcc tgctggatga ccccgccggc ggcctgccgg cgcaccgcgg
      781 acaggagagc cacgtgggcc tcccaggggc gggggtcctt gccgatgagc agcggcgcga
      841 gccccgcgat cgcggcggag acgccgaccc cgccgaagga ctcgttgtac tcgctccacc
      901 cgacgaggcc ctcgtcggtg ctgatcttga ggaagtcgag gctgcgccag cccccgtcgg
      961 cgtgcagcga ttccaccgtg gtgatcttca tcgcgtctcc ttggctactg cttgaccgcg
     1021 ccgaacgtga ggccggagac gatgtgccgc tgcgcgacga gcgcgaccag gagcaccggc
     1081 agcgtgatga gcgtggcggc cgcggccagg gtgccccagt tgatctcctc gtaggagatc
     1141 atggtgtaga ccgcgatggg caaagtgcgc gtggcgcggc cggcgaggat caccgagaag
     1201 aggaagttgt tccaggagaa gacgaaggag aggatggcgg tcgccaccac acccggcttg
     1261 accagcggca gggcgatgcg ccagaacgcg ctccagtagg agcagccgtc gatgagcgcc
     1321 gcgtcttcca tctccgcggg gacgtcctcg aagaacgaga tcatcaccca gatgatgatg
     1381 ggcaggctca cgatgagatg cgtcaggatc agcgcgccgt aggtgtccac catccggagc
     1441 tggcggaaga agatgtacca ggggatgagg taggcgatgc cgggaatgat gcgcgccacc
     1501 aggatgacca cggccagccg ctgctggcgc cagcgcgcga tcgagtaggc ggcggggagc
     1561 ccgaccacca ggccgagcgc ggtgctgccc gccgccacca ccaggctgtt ccacgtgtag
     1621 aggaagaagg ggtacttggt gaacacctcg cggtagccgg ccgcggtgac gctgaagcgc
     1681 acgaacgccg gcgggtacgc catcgcctcg acctggggct tgagcgacag cgtgatcatc
     1741 cagtagaaga cgaagacggt gggcagcatg aacaatgagg ccaaggcgta gaagccggcg
     1801 gtggacagcc aggggtgccg gccgcgcctc accacgtcgt ggtcctccgg accttgatca
     1861 ggatcagcga ggcgcccatg accaccgcga agagcgcgac cgccatggac gaggcgtagc
     1921 ccatgttgaa ataggagaag gcctggttga acagcaggat gttgatggtc tccgaggcgt
     1981 tggcgggccc cccctgggtc atcacgaaga tgatgtcgaa ggtcttgagc gcgtcgatgg
     2041 cgcggaagag cacggccacc acgagcgcgg gccggagcag cgggagcgtg atgtgccaga
     2101 acgcccgcag cgtcgaggcg ccgtcgatgt gggcggcctc gtagggctcg cggggcaggc
     2161 tggccagccc ggccagcgcg atgagcatga tgaggggcgt ccactgccac acgtcgacca
     2221 gcgcgagcgc gtacagcgcc gtctggctcg agtacgtcca gcgccacggg gagagcccga
     2281 cgacggagag caggtagttc gccacgccga ggctcgggtg atacatcatc acgaacacga
     2341 gcccgatggc cgtcggggtc gccaccatgg gcaggatggc cagcgtgcgc agcagacccc
     2401 gcccccagaa ctgccggttg aagagcagcg ccatggcgag gccgaggatc gtctcggcgc
     2461 cgacagccac gatggtgaag tagacggtcc gccccaccgc ctcctggaag cgcgcgtcag
     2521 cgagcgcgat cttgcggtag ttcgcaagac cgatgaagtg cggtgcggtc aggctggagg
     2581 cgaaccactc ctggaggctc atccataccg tgtaggcgat gggatagacg atgatcaccg
     2641 ctacgagcag cacagctgga gcgggaaaca gccaccgcgc gcgtcggtgt gcccagcggc
     2701 cgagccgacc tgagaaggtc ggcaccccgc ctggcgccag gagcctgttc aagtagttcc
     2761 catgctctcg cgagcaagaa tgctcctatc tagccctccg tcctgttgag catctcctgg
     2821 aagtccttct gggcttgcgc caggacctgg gcgggcggcg cgccctcgat ggccttctg
//
LOCUS       DATMCV010000240         5677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_346466, whole genome shotgun sequence.
ACCESSION   DATMCV010000240 DATMCV010000000
VERSION     DATMCV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346466"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VNU03_05615"
     CDS             <1..591
                     /locus_tag="VNU03_05615"
                     /inference="COORDINATES: protein motif:HMM:NF020671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HWP73968.1"
                     /translation="LLEDFHREYPLRSGISREELRSRAGHAQERVFAQLLGALETEAV
                     VRSERDQVRLASHAIRLSPEQERVVKGLEADFRSGGAAPPSPEEALGRHGVKGTEKHE
                     LFQVLVADRTLVRVKDSLFYHADALRVVQDQLVILLRQKKEIGPADFKDLFGVSRKFA
                     IPLMEYFDAQRVTVRVGERRVLREGYGRVDNPGTGA"
     gene            628..816
                     /gene="tatA"
                     /locus_tag="VNU03_05620"
     CDS             628..816
                     /gene="tatA"
                     /locus_tag="VNU03_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWP73969.1"
                     /translation="MFGLGYQELLLILVIVLILFGAQRLPDLARSLGSSVKEFKKGVT
                     ELKDDSTAAAKKDDEKKA"
     gene            949..2601
                     /locus_tag="VNU03_05625"
     CDS             949..2601
                     /locus_tag="VNU03_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HWP73970.1"
                     /translation="MLAGMASGGRRAAARLAPHGDLAVALGLAVVTLLSRWPYRARML
                     YNWDAVQFALALHEFDIAKHQPHPPGYLLYVALGRLLNASLGDPTLAYVALAMLFSAG
                     TTFVLYWLARRLFDRATALIAASLLAVSPLFWFYGSVGLTYAGEAFAASVVAWFAYGA
                     LTGHVSSLYWGALALGLTGGMRQSVLLLLLPLWLGSALLGIRSRRRVAIAGAILLATV
                     LAWLLPMLWLTGGPAAYVGASTQLYGSVLLPTSVLGGSLEVTLAQARYLLESTLVGLG
                     PLGLVALALPAYARRMGWSRPEWFLLGWIVPPIIFYMLIHFGQAGYVLTFLPALVILL
                     SRVLAWVVAAGSERLRRPNWRWALTTATLLPLVLINTGFFVSARPLPREFNNRGGDGW
                     VWRARDEFHDWIMSRTAAALREHEAVIRTYVETIRAVYDPSDTALVTELGNPRSYPWL
                     RHAMFYMPEYPTYQVQVGAMPLGFYAPQYAATMILTPGARITLPAGMRQLVWFVDHWD
                     PATQPPAGLREVQLPYGRFLYVLPLTRTPVEYAGYTFERAAR"
     gene            2598..4190
                     /locus_tag="VNU03_05630"
     CDS             2598..4190
                     /locus_tag="VNU03_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73971.1"
                     /translation="MTGRALTLGAAAAALGLGVVVFLWKTRQIAGSIGVGAFPLDDSW
                     IHMQFARNLAEGRGFAYNPGVPVSGSTAPLWTLALGGVFTVFGPHPALAKALGLGLAL
                     ATAWLTGSLAELWTGRRELGLLALVLTALAGPMLWGALSGMEVSLAAFLVTTALVLRA
                     REREGAAAVALGLAALARPEAILLLPLFWLSGSLTWRRAVTWLGPVAACLAPWVAFNL
                     VTIGGPLPGTAVAKIEGGLLGALSGVREPLATSLVSRPGQYTWEWIRWLWRVDALLPL
                     LLVPGIAWLGRRLGRAALIPACVLLAHPLAMALLAPYRGPGFQEGRYSIHLLPLAIAT
                     AVAPLAAVTAVRARGVAALALLVGALVALPGAASRYGWAVQNIEAMQVHLAHWVTDHT
                     PPAARLGLNDVGAITYFSRREIVDVMGLVTPAILPYRREGESGVLRYLEQACPDYLIV
                     FPEWFPTISAMTDRFRPVYRVRLDHNTVAGADELVVYEAIWSRWAADRHPCPGALAVA
                     PGPAGPGAAEKGRDRIIVTWSP"
     gene            4187..4819
                     /locus_tag="VNU03_05635"
     CDS             4187..4819
                     /locus_tag="VNU03_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HWP73972.1"
                     /translation="MKGLGRVLAAGMVLGALVCGAAAPASAQIYRWTDERGEVRFSQG
                     INSVPPQARGGAVMMSTPGQPAPSAPAPAETPASTAAPAGAARIPFTPGRPIMVNARI
                     NGGGTTQLILDTGAQGTVISPTALAALGVSYRNAARGSIRGVTGETSVLAVRIESLEV
                     EGARFGPLMVISHDAGLGSGADGLLGRDFLDNFIVTIDSSSRVVTLTPKK"
     gene            4913..>5677
                     /locus_tag="VNU03_05640"
     CDS             4913..>5677
                     /locus_tag="VNU03_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWP73973.1"
                     /translation="MDYKDTLNLPKTAFPMKANLPKLEPEWLERWAAMDIYGRIRQAS
                     ASRPLWILHDGPPYANGHIHMGTALNKILKDLVVKSRSMLGHNAVYVPGWDCHGLPIE
                     HQVDKELGLDQASVDVRQAMDPLEKRRRCREYASKFIDIQREEFRRLGVFGDWRDPYR
                     TMAPAYQATIAREFGRFVGRDLVHKGLKPVHWCMHCKTALAQAEVEYEDQTTPSVYVK
                     FPLKASAPVLAALGSRPVSLVIWTTTPWTLPANLAIA"
BASE COUNT          705 a         2103 c         1959 g          910 t
ORIGIN      
        1 ctgctcgagg acttccaccg ggagtacccg ctgcggagcg ggatctcccg cgaggagctg
       61 cgcagccggg ccggccacgc ccaggagcgg gtcttcgccc agctcctcgg cgcgctcgag
      121 acggaagcgg tggtccggag cgagcgcgac caggtccggc tggcctccca cgcgatccgc
      181 ctgagcccgg agcaggagcg ggtggtcaag gggctcgagg ccgacttccg gagcggcggc
      241 gcggcgcccc cgagtccgga ggaggcgctg ggtcgccacg gggtcaaggg caccgagaag
      301 cacgagctct tccaggtgct ggtcgcggac cggacgctcg tgcgggtcaa ggactcgctc
      361 ttctaccacg ccgacgccct gcgcgtggtc caggaccagc tcgtcatcct cctgcgccag
      421 aagaaggaga tcggtcccgc cgacttcaag gacctcttcg gggtcagccg gaagttcgcc
      481 atcccgctga tggagtactt cgacgcccag cgcgtcacgg tccgggtcgg agagcgtcgg
      541 gtgcttcgcg aggggtacgg gcgcgttgac aaccccggga ccggcgcgta gtgtaagggg
      601 gcgtcagtaa aggaaaggag tcccgccatg tttggtctgg gatatcaaga gctgctgttg
      661 atcctggtga tcgtgctgat cctgttcggc gcgcagcgtc tgccggatct cgcgcgctcg
      721 ctcggcagca gcgtcaagga gttcaagaag ggcgtgaccg agctgaagga cgattcgacg
      781 gcggccgcga agaaggacga cgagaagaag gcgtagcccg gtttcctctc tcccccacac
      841 actgctcggg tcgccgatgt atcaggtcct cttcgacgtc ggcacaggct cgagcggcgg
      901 ccgctccgtg atccctcgcg tctcgccgcc ccttccgtga tccgaccgtt gctcgccggc
      961 atggcgtcgg gcggccgacg ggccgccgcg cgcctggccc cccacggcga cctcgcggtc
     1021 gcgctcggcc tcgcggtggt caccctgctc tctcgctggc cctaccgcgc gcggatgctc
     1081 tacaactggg acgcggtcca gttcgcgctg gcgctccacg agttcgacat cgccaagcac
     1141 cagccgcacc cgcccggcta ccttctgtac gtcgcgctcg gccgcctgct caacgcctcg
     1201 ctcggcgacc ccacgctcgc ctacgtggct ctcgcgatgc tcttcagcgc gggcactacc
     1261 ttcgtcctct actggctcgc gcgccggctc ttcgatcgcg ccaccgcgct catcgccgcc
     1321 tcgctgctcg cggtgagccc gctcttctgg ttctacggct cggtggggct cacctacgcg
     1381 ggggaggcgt tcgcggcctc ggtggtggcg tggttcgcct acggggcgct caccggccac
     1441 gtgtcgtcgc tctactgggg cgcgctcgcg ctcgggctga ccggcgggat gcggcagtcg
     1501 gtgctcctcc tgctgctgcc gctctggctc ggctcggccc tgctcggcat ccggtcacgc
     1561 cggcgggtgg ccatcgcggg cgcgatcctc ctggcgaccg tgctcgcctg gctgctgccg
     1621 atgctctggc tgaccggggg cccggccgcg tacgtgggcg cgtcgaccca gctctacggg
     1681 tcggtgctgc tcccgacctc ggtgctgggc ggctcgctcg aggtcacgct cgcccaggcc
     1741 cgctatctcc tcgagtccac cctggtgggg ctgggcccgc tcggcctggt ggcgctggcg
     1801 ctgcccgcct acgcgcggcg gatgggctgg agccgccccg aatggttcct tctcggatgg
     1861 atcgtcccgc cgatcatctt ctacatgctc atccacttcg gtcaggctgg ctacgtgctg
     1921 accttcctgc ccgccctggt gatcctcctg agccgcgtgc tcgcctgggt ggtcgcggcg
     1981 ggctcggagc ggctgcgccg tcccaactgg cgctgggccc tcaccaccgc gacgctgctg
     2041 cccctggtgc tgatcaacac cgggttcttc gtgagcgcgc ggccgctgcc gcgcgagttc
     2101 aacaatcgcg ggggcgacgg gtgggtgtgg cgggcgcgtg acgagttcca cgactggatc
     2161 atgagccgca ccgcggccgc gttacgcgag cacgaggcgg tgatccgcac ctacgtggag
     2221 accatccgcg cggtctacga tccgagcgac accgcgctcg tgaccgagct cggcaacccg
     2281 cgctcgtatc cgtggctgcg tcacgccatg ttctacatgc cggagtaccc gacctatcag
     2341 gtgcaggtgg gcgcgatgcc gctcggcttc tacgccccgc agtacgcggc caccatgatc
     2401 ctgaccccgg gcgcccgcat cacgctcccg gccgggatgc gtcagctcgt ctggttcgtc
     2461 gatcactggg acccggccac ccagccgccg gccggcctgc gcgaggtgca gctgccctac
     2521 gggcgcttcc tctacgtcct gcccctcacc cgcacgcccg tggagtacgc cggctacacc
     2581 ttcgagcgcg ccgcgcggtg accggacgcg ccctgaccct cggcgcggcc gccgccgcgc
     2641 tcgggctcgg cgtcgtggtc ttcctctgga agacccgcca gatcgccggc tcgatcggcg
     2701 tcggcgcgtt tcccctcgac gactcgtgga tccacatgca gttcgcccgc aacctggccg
     2761 agggccgcgg cttcgcgtac aacccgggcg tgccggtgtc ggggtcgacg gccccgctgt
     2821 ggacgctcgc gctcggcggc gtcttcacgg tcttcggccc ccatccggcc ctggccaagg
     2881 ctctgggact gggcctggcc ctggcgaccg cctggctcac cggatccctc gcggagctct
     2941 ggaccggccg ccgcgagctc gggctcctgg ctctggtcct cactgccctc gcggggccga
     3001 tgctgtgggg cgcgctctcg ggcatggagg tctcgctcgc cgccttcctg gtgaccaccg
     3061 cgctggtgct ccgagcccgg gagcgcgagg gcgcggccgc ggtcgcactc gggctggccg
     3121 ccctcgcgcg gccggaggcg attctcctgc tccccctctt ctggctgtcc ggatcgctca
     3181 cgtggcgtcg cgcggtcacg tggctcggcc cggtcgcggc ctgcctcgcg ccgtgggtcg
     3241 ccttcaacct ggtgacgatc ggcggtccgc tgcccggcac cgcggtggcg aagatcgagg
     3301 ggggactgct gggtgcgctc tcgggggtcc gcgagccgct cgccacctcg ctcgtgagcc
     3361 ggcccggcca gtacacctgg gaatggattc gctggctctg gcgcgtggat gcgctcctgc
     3421 cgctgctgct cgtgcccggc atcgcctggc taggccggcg cctgggccgc gcggcgctca
     3481 tccccgcctg cgtgctgctg gcgcatccgc tggcgatggc gctgctggcg ccctaccggg
     3541 gacccgggtt ccaggagggc cgctactcca ttcacctgtt acccctggcg atcgcgacgg
     3601 ccgtcgcgcc gctggccgcg gtcaccgcgg tgcgggcccg cggggtggcg gccctcgccc
     3661 tgctggtggg cgcgctcgtg gccctgcccg gggcggcgtc acgctacggc tgggcggtcc
     3721 agaacatcga ggccatgcag gtccacctgg cgcactgggt caccgaccac accccgccgg
     3781 ccgcccggct cggcctcaac gatgtgggcg cgatcaccta tttctcccgc cgcgagatcg
     3841 tcgacgtcat ggggctggtg accccagcca tcctcccgta ccgccgggaa ggggagagcg
     3901 gcgtgctccg gtatctcgag caggcgtgcc ccgactacct gatcgtcttc ccggagtggt
     3961 tcccgaccat ctcggcgatg accgaccggt tccggccggt ctaccgcgtg cgcctggacc
     4021 acaacaccgt cgcgggagcc gacgagctgg tggtctacga ggccatctgg agccgctggg
     4081 cggcggaccg gcacccttgc ccaggtgccc tggcggtcgc cccgggccct gccggcccgg
     4141 gtgccgcgga aaaaggccgg gaccgtataa tcgtcacgtg gagcccatga agggtctcgg
     4201 tcgggtcctc gccgccggta tggtgctggg cgccctcgtc tgcggcgcgg ccgcccccgc
     4261 ctcggcccag atctaccgct ggaccgacga gcggggagag gtgcggttct cccaggggat
     4321 caacagcgtc ccgccccagg cccgcggcgg cgccgtcatg atgagcaccc ccggtcagcc
     4381 ggccccgtcg gcgcccgcgc ccgccgagac cccggccagc accgccgcgc cggccggcgc
     4441 tgcccgcatc ccgttcaccc cgggccgccc catcatggtg aacgcccgga tcaacggagg
     4501 cggcaccacc cagctcatcc tcgacaccgg cgcccagggc acggtgatca gccccaccgc
     4561 gctcgcggcc ctcggggtca gctaccgcaa cgcggcccgc ggctccatcc gcggcgtcac
     4621 cggggagacc agcgtcctcg cggtgcggat cgagagcctc gaggtggaag gcgcccgctt
     4681 cggtccgctg atggtcatct cccacgatgc cgggctcggg tcgggggccg acggcctgct
     4741 cgggcgggac ttcctcgaca acttcatcgt cacgatcgac agctcgagcc gcgtcgtcac
     4801 cctgaccccg aagaagtagt accgacgtag cctcaggcgt cgcccgacgc cgcccgcctc
     4861 ggacggcccc attcttgccg ggagcaggga tgagtgctag cgttggccct ctatggatta
     4921 caaagacacg ctgaacctgc cgaaaaccgc ctttcccatg aaggccaacc tgcccaagct
     4981 ggagccggaa tggctcgagc gctgggcggc gatggacatc tacgggcgga tccggcaggc
     5041 ctcggcgagt cgtcccctct ggatcctgca cgacggcccg ccgtacgcca acggccacat
     5101 ccatatgggg accgcgctca acaagatcct gaaggatctg gtggtgaagt cgcgctccat
     5161 gctcgggcac aacgcggtct acgtgccggg ctgggactgc cacgggctgc cgatcgagca
     5221 ccaggtggac aaggagctgg ggctcgacca ggcctcggtc gacgtgcggc aggcgatgga
     5281 tccgctcgag aagcgccggc gctgccgcga gtacgcgagc aagttcatcg acatccagcg
     5341 cgaggagttc cgacgcctcg gggtcttcgg cgactggcgt gatccgtacc ggacgatggc
     5401 gcccgcctac caggcgacca tcgcgcgcga gttcggccgc ttcgtggggc gcgacctggt
     5461 gcacaagggg ctcaagccgg tgcactggtg catgcactgc aagaccgcgc tggcccaggc
     5521 cgaggtggag tacgaggacc agaccacgcc ctcggtctac gtgaagttcc ccctcaaggc
     5581 gtccgcgccg gtcttggccg ccctcggcag tcgcccggtc tcgctcgtga tctggaccac
     5641 gacgccgtgg accttgcccg cgaatctcgc catcgcg
//
LOCUS       DATMCV010000241         3280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_946861, whole genome shotgun sequence.
ACCESSION   DATMCV010000241 DATMCV010000000
VERSION     DATMCV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946861"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VNU03_05645"
     CDS             complement(<1..773)
                     /locus_tag="VNU03_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73974.1"
                     /translation="MRRLLLMGALSLAAASPASGQEASGASLRGDCQDRLAEYLKKPS
                     PGYFFYVEAPGSRKYACDGSVEERGEFDRYPGSAQRAFTACQNAADDSGITARCEVIA
                     RGPAIVARSYAEAQGRDDAAGLVRDAMRCGQPPLGRWFWAERAFCDMRWHGAASANGV
                     VIWNHGIHGTVGQHAAPVPPVLRLLQARGWDVVKIARNNLGETTGEQSLNRAVRRTQE
                     EIAARRREGYGKVVLAGQSFGGYITLETAEASRDVYGVVA"
     gene            complement(773..1252)
                     /locus_tag="VNU03_05650"
     CDS             complement(773..1252)
                     /locus_tag="VNU03_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73975.1"
                     /translation="MRATALALLLALGGLSIGPPARGEEIPRLTFAQLADSMRANLGV
                     TQGIKAYNGREIEIQGFIIPAGPPDLSFFLLSRVSGTGNYCCELPSGQDETVYVYTAR
                     GVSLRYDPVRVYRIRGVFEAGHQVDRSYGVSFFRVRDARVEEAVGARIFKVGETKGP"
     gene            complement(1257..2444)
                     /locus_tag="VNU03_05655"
     CDS             complement(1257..2444)
                     /locus_tag="VNU03_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP73976.1"
                     /translation="MTPVTVVWRYLQGRFVTSVLTVVSVALGVSLVIASVLLTRGIKE
                     GFIAGATDYNLIVGAKGSPTQLVLNVVFRMDAPTPNIPLSAYEDLRADPRVEAAVPVA
                     MGDAYQGFRYVATSDAYFAPLPWRRHVPVLATGRLFRSDAPERPDYEAVLGADAARGT
                     GLKPEDRFYEGEEMAAYPLRVVGVLRPTGTADDRAIFISLASYWEMNEISRKALIKPL
                     TAVLIRPKRLSDLTALHRGWNVGPDLQAALPSAILLNIFNLLGLVEDVLAVVLAVVVV
                     VVGLYLFVTMYNATLERRREIATMRALGARRATVLGIVLLESCVIAGLGGLAGILGGH
                     GVAALGASLLAARGGPVTHALALSALQPVTFGAVVALGALAGLLPAVLAYRTEVAENL
                     APL"
     gene            complement(2441..3142)
                     /locus_tag="VNU03_05660"
     CDS             complement(2441..3142)
                     /locus_tag="VNU03_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010503566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP73977.1"
                     /translation="MTPAAPVLSLRGVTKSFRLADGGALQVLDVEHLELPAGGCTVIR
                     GRSGSGKTTLLNLIAGVSAPTGGTIVVHGTEITALPEARRDRFRAERIGYVFQTFNLL
                     PAFSALENVVLAMMFARAVPRREQRTRARAILARLGLSARLGHKPGQLSRGEQQRVAI
                     ARALANDPPLILADEPCASLDAATAREVLAVLLAVCREAAKTLLVVSHDEAVLGAGDR
                     LLDMGTINARAGAAG"
BASE COUNT          488 a         1221 c         1148 g          423 t
ORIGIN      
        1 gcgacgacac cgtaaacatc cctggaggcc tccgccgtct ccagcgtgat gtagccgccg
       61 aacgactgcc cggccagcac gacctttccg tatccctcgc ggcggcgcgc ggcgatctcc
      121 tcctgggtcc gccggacggc ccggttgagg gattgctcgc cggtggtctc cccgagattg
      181 ttgcgcgcga tcttcaccac gtcccagccg cgcgcctgca agagccggag gaccggcggc
      241 accggcgccg cgtgctgccc caccgtcccg tgaatgccgt ggttccagat caccaccccg
      301 ttggcggagg cggccccgtg ccaccgcatg tcgcagaacg cccgctcggc ccagaaccac
      361 cggccgagcg gcggctggcc gcagcgcatc gcgtcccgga ccaggccggc cgcatcgtcc
      421 cgcccctgcg cctcggcgta ggagcgggcc acgatcgcgg ggccgcgcgc gatcacctcg
      481 caccgcgccg tgatgccgct gtcgtcggcc gcgttctggc acgcggtgaa cgcgcgctgg
      541 gccgacccgg ggtagcggtc gaactcaccc cgctcctcga cgctcccgtc acacgcgtac
      601 ttcctcgaac cgggggcctc gacgtagaag aagtagccgg ggctcggctt cttcaagtac
      661 tcggcgaggc gatcctggca atcgccgcgg agcgatgctc cgctcgcctc ctggccggag
      721 gcgggtgacg ccgccgcgag ggagagcgcg cccatcagca ggagtcggcg catcacggcc
      781 ccttggtctc gcccaccttg aagatgcgcg cccccaccgc ctcctcgacg cgcgcgtcgc
      841 gcacccggaa gaagctgacc ccgtagctcc ggtccacctg gtggccggcc tcgaagacgc
      901 cgcggatccg gtagacccgg accgggtcgt agcggaggga gactccccgc gcggtgtaca
      961 cgtagaccgt ctcgtcctgc ccgctcggta gctcgcagca gtagttcccc gtgcccgaga
     1021 cgcggctcag caggaagaac gacaggtcgg gcgggcccgc gggaatgatg aacccctgga
     1081 tctcgatctc ccgcccgttg taggccttga tcccctgggt cactccgagg ttcgcccgca
     1141 tcgagtccgc gagctgggcg aaggtgaggc gcgggatctc ctcgccccgc gccggcggtc
     1201 cgatcgagag accaccgagc gccagcagga gcgcgagcgc cgtcgcgcgc atccgatcag
     1261 aggggcgcaa ggttctcggc gacctccgtg cgataggcga gcaccgccgg cagcaagccg
     1321 gccagcgccc ccagggcgac gaccgccccg aaggtcaccg gctggagcgc gctcagggcg
     1381 agggcgtgcg tgaccggccc accccgcgcc gcgagcaggg aggcgccgag agccgcgacg
     1441 ccgtggccgc ccaggatccc ggcgagcccg ccgaggcccg cgatcacgca ggactccagc
     1501 aggacgatgc cgagcacggt cgcccggcgg gcgcccagcg cgcgcatcgt ggcgatctcc
     1561 cgccggcgct cgagggtggc gttgtacatg gtcacgaaca ggtagagtcc gaccacgacc
     1621 accacgaccg cgagcaccac cgccagcacg tcctccacca gacccagcag gttgaagatg
     1681 ttgagcagga tcgcgctggg aagggccgcc tgcagatccg gcccgacgtt ccacccgcga
     1741 tgcagcgcgg tcaggtcgga gagacgcttc ggccggatca ggaccgcggt gaggggcttg
     1801 atcagcgcct tccgcgagat ctcgttcatc tcccagtagc tcgcgagcga gatgaagatg
     1861 gcgcgatcgt cggcggtccc ggtgggccgg agcacgccga ccacccggag cgggtacgcg
     1921 gccatctcct cgccctcgta gaagcggtcc tccggcttga ggcccgtccc gcgggcggcg
     1981 tcggccccga gcaccgcctc gtagtccggc cgctccggcg cgtcgctccg gaacagacga
     2041 cccgtcgcca ggacgggaac gtgccgccgc cacggcaagg gcgcgaagta ggcatcgctc
     2101 gtcgccacgt agcggaaacc ctggtacgcg tcgcccatgg ccaccggcac cgcggcctcg
     2161 acgcgcggat ccgcgcggag atcctcgtag gcgctgagcg ggatgttcgg ggtcggcgcg
     2221 tccatgcgga agacgacgtt cagcacgagc tgggtcgggc tgcccttggc gccgacgatc
     2281 agattgtagt ccgtcgcccc cgcgatgaac ccctccttga tgccgcgggt cagcagcacg
     2341 ctcgcgatga ccaggctcac ccccagggcg accgagacca cggtcaggac gctggtgacg
     2401 aagcgcccct ggagataccg ccacacgacg gtgaccggcg tcacccggcc gccccggccc
     2461 gcgcgttgat cgtccccatg tcgagaagcc gatcgcccgc gccgaggacc gcctcgtcgt
     2521 gcgagacgac gagcagggtc ttggccgcct cccggcagac cgcgaggagg accgcgagga
     2581 cctcgcgcgc ggtcgccgcg tccaggctgg cgcagggctc gtcggccagg atcagcgggg
     2641 ggtcgttcgc gagcgcccgc gcgatcgcca cccgctgctg ctccccccgc gagagctgtc
     2701 ccggcttgtg gccgaggcgc gcgctgagcc ccaggcgggc gaggatcgcg cgggcccggg
     2761 tgcgctgctc ccgccgcggg accgcgcgcg cgaacatcat cgcgagcacg acgttctcga
     2821 gcgcgctgaa ggcgggcagg aggttgaagg tctggaagac gtagccgatc cgctccgccc
     2881 ggaaccggtc acgccgcgcc tcgggcagcg cggtgatctc ggtgccgtgg acgacgatcg
     2941 tgccgccggt gggcgccgac actcccgcga tcaggttgag cagcgtggtc ttgcccgagc
     3001 cgctgcggcc cctgatcacc gtgcagcccc ccgcgggcag ctccaggtgc tcgacgtcga
     3061 gcacctggag cgccccgccg tccgcgaggc ggaacgactt cgtcaccccg cgcaggctca
     3121 ggaccggcgc cgccggggtc atgcctcttg ctaccggatg gcgatggggt tcaccggcac
     3181 cccgatcccg ccgcgcagca ggagcggagc gacgacgaag aggaactcgt aggcctggtc
     3241 cttggccagc tcgtccaggt tcatgatctc gaggatgtag
//
LOCUS       DATMCV010000242         2730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_414797, whole genome shotgun sequence.
ACCESSION   DATMCV010000242 DATMCV010000000
VERSION     DATMCV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414797"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VNU03_05665"
     CDS             complement(<1..936)
                     /locus_tag="VNU03_05665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73978.1"
                     /translation="MATAPAGGPGRYRWVVVGVSTAVNALAWGARSTFALFFVAMLEE
                     FGWGRGPTALGYSLSWLAFVVFAPAAGWLGDRAGARAVVATGGLILGGALVLTGRATS
                     RADYYLCFGVLAAAGTAAIIVPATTIVTRWFVRARGTAMGILSTGGPASAVAFYPVNA
                     WLITALGWRRALAVFGCLVAAATISLALLYRDPPASGDGSVRDPRPGPPRHADLRAEE
                     WTLRRALRSGRLWAAFVMTAFGVIGFQIMATHQVAHAVDRGVPPVTVVWLFSFGALCM
                     MAGNLLGGWLSDRLGPGRVFTLGSIVAIAGIACLAA"
     gene            1004..1702
                     /locus_tag="VNU03_05670"
     CDS             1004..1702
                     /locus_tag="VNU03_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010451468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWP73979.1"
                     /translation="MDAEVSIASVAGLVADPARARMLTALMDGRARTAKELAYGARVT
                     PQTASSHLAKLLAAGLLAMERQSRHRYFRLASPSVGHAIEALMAVSSVPPRAARPAPA
                     GPLEGLRLARTCYDHLAGRLGVSVTDAMVRRHWLTPGERDYRLTAAGARVLARLGVDV
                     DRTRRERRAFAQQCLDWSERRTHLAGSLGAAVARACLELHWVQRVAEERTLRLTPKGQ
                     RGLRTWFGITWRLP"
     gene            1711..>2730
                     /locus_tag="VNU03_05675"
     CDS             1711..>2730
                     /locus_tag="VNU03_05675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP73980.1"
                     /translation="MYQGWKVVGCAFLIAVFGWGFGFYGIGVFLAELVERHGWATGSV
                     ASAVTILYLVGAVLIAFIGSAFERFGPRRVVLAGMSAMGAAAVGLTWITQPWHLYLVF
                     PLMAMGWAAMSGAALNVIVAPWFERRRGLAISIAFNGAAVGGVLLVPALVFLIGRIGF
                     RPAVLFLVALMVAVLTPLVLWLLARGPEAVGLGPDGDPPAARAPAPKAAGVAAPRREL
                     MRTWHFWSVALPFALGLTAQISLIIHQVSFLKPTLGTEGAAWAVSLTSVCAVIGRLAV
                     GSFVDRVNRRAVIAGNFLLQAASIGLMLSDPGPALLYVACAMFGLGVGNTTSLPSVIV
                     QAEFPK"
BASE COUNT          349 a          982 c         1030 g          369 t
ORIGIN      
        1 cgcggcgagg caggcgatgc ccgcgatcgc gacgatggag ccgagggtga acacccggcc
       61 gggcccgagc cggtcggaga gccagccccc gagaagattg ccggccatca tgcagagagc
      121 cccgaaggag aagagccaca ccacggtgac cggtgggacg ccgcggtcca ccgcgtgggc
      181 gacctgatgg gtcgccatga tctggaatcc gatgaccccg aaggcggtca tgacgaacgc
      241 ggcccagagc cggccgctcc gcagcgcgcg ccggagcgtc cactcctcgg cgcggagatc
      301 cgcgtgccgc ggcggcccgg gccgcgggtc gcgcacggac ccgtcaccgg aggcgggcgg
      361 gtcccggtag aggagcgcga gcgagatcgt cgcggccgcg accaggcacc cgaagaccgc
      421 gagcgcccgg cgccagccca gggcggtgat gagccaggcg ttcaccgggt agaaggccac
      481 cgcgctggcc ggaccgcccg tgctcaggat ccccatcgcg gtcccgcgcg cacgcacgaa
      541 ccagcgcgtg acgatcgtgg tggcggggac gatgatcgcg gcggttcccg ccgcggcgag
      601 gacgccgaag cacaggtagt agtcggcccg cgacgtcgcc cggccggtga ggacgagagc
      661 gcccccgagg atcaatccgc ccgtcgccac caccgcccgc gcccccgccc gatcgccgag
      721 ccagccggcc gccggcgcga agacgacgaa ggcgagccag ctcagcgaat agccgagcgc
      781 ggtcgggccg cgcccccagc cgaactcctc cagcatcgcg acgaagaaca gcgcgaaggt
      841 ggagcgcgcc ccccacgcga gcgcgttgac cgcggtggag acccccacca cgacccatcg
      901 gtatcgtccg ggcccgcccg ccggcgccgt cgccatcctg ctctcgacgg tgcgcgccgc
      961 ccgggcgctt gtaaagcaca gactagtcga ctaccatcga actatggatg cagaggtcag
     1021 catcgcgtcg gtggcgggcc tggtggcgga tccggcccgc gcccggatgc tcaccgccct
     1081 gatggacggc cgcgcgcgga ccgcgaagga gctggcctac ggcgcccgcg tcacgccgca
     1141 gaccgcgagc agccatctcg cgaagctgct cgcggccggc ctgctcgcga tggagcgcca
     1201 gagccgtcac cgctacttcc ggctggcctc gcccagcgtg gggcacgcga tcgaggccct
     1261 gatggcggtc tcgtccgtcc cgccgcgcgc ggcccggccg gctcccgcgg ggcccctcga
     1321 ggggctccgg ctggcccgga cctgctacga ccatctggcc gggcgcctcg gggtgagcgt
     1381 cacggacgcg atggtgcgcc gtcactggct cacgccgggg gagcgcgact atcgcctgac
     1441 cgcggcgggc gcgcgcgtcc tcgcccgtct gggcgttgac gtggacagga cccggcgaga
     1501 gcgccgggcg ttcgcgcagc agtgtctcga ctggagcgag cgccgcacgc acctcgcggg
     1561 ctcgctcggc gccgcggtgg ctcgcgcctg cctcgagctg cactgggtgc agcgggtggc
     1621 ggaggagcgc acgctccggc tgaccccgaa ggggcagcgg ggcctgcgga cgtggttcgg
     1681 tatcacctgg cgcctcccgt agcctcgcgc atgtaccagg gatggaaggt cgtcggctgc
     1741 gcgttcctga tcgcggtgtt cgggtggggg tttggcttct acgggatcgg ggtcttcctg
     1801 gccgagctgg tcgagcgcca cggctgggcg acgggctcgg tggcgtccgc ggtcaccatt
     1861 ctctacctgg tgggggcggt cctgatcgcg ttcatcggga gcgcgttcga gcgcttcggc
     1921 ccgcgccggg tggtgctggc cggcatgtcg gccatggggg cggccgccgt cgggctcacg
     1981 tggatcacgc agccgtggca cctctacctg gtgtttccgc tgatggcgat gggatgggcg
     2041 gccatgagcg gggcggccct caacgtcatc gtcgcgccct ggttcgagcg ccgacggggc
     2101 ctcgccatca gcatcgcgtt caacggcgcg gcggtcggcg gagtcctgct cgtgccggcg
     2161 ctcgtgttcc tcatcgggcg gatcggattc aggcccgcgg tgctgttcct ggtcgcgctg
     2221 atggtcgcgg tgctcacccc gctcgtgctg tggctgctcg cgcgcgggcc cgaggcggtg
     2281 gggctggggc cggacggcga tccgcccgcg gcccgggcgc cggccccgaa ggcggccgga
     2341 gtcgccgcgc cgcggcggga gctcatgcgc acctggcact tctggtcggt cgcgctgccg
     2401 ttcgccctcg ggctgaccgc gcagatcagc ctcatcatcc accaggtgtc ctttctcaag
     2461 ccgacgctcg ggacggaggg cgccgcctgg gcggtgagcc tgacttccgt ctgcgcggtg
     2521 atcggacggc tggcggtggg ctcgttcgtg gaccgcgtga accgccgcgc cgtgatcgcg
     2581 ggcaacttcc tgctgcaggc ggcctcgatc gggctgatgc tctccgaccc ggggccggcc
     2641 ctgctctacg tcgcctgcgc gatgttcggg ctcggagtgg gcaacaccac gagcctgccg
     2701 agcgtcatcg tgcaggcgga gttccccaag
//
LOCUS       DATMCV010000243         3187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_613698, whole genome shotgun sequence.
ACCESSION   DATMCV010000243 DATMCV010000000
VERSION     DATMCV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613698"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VNU03_05680"
     CDS             complement(<1..356)
                     /locus_tag="VNU03_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP73981.1"
                     /translation="MHIKDLYSIVVTEKRVECRDFYVRWFGFQVVFEASWFVYLAASG
                     DHPFGLAFMTPDHPSRPPGRDVFNGKGLLLTLQVEDARAEFERLKGAGLEIAYPLHDE
                     PWGQRRFAVHDPAGTW"
     gene            complement(388..684)
                     /locus_tag="VNU03_05685"
     CDS             complement(388..684)
                     /locus_tag="VNU03_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP73982.1"
                     /translation="MQAGADEEKVREVPTWRESARFSPVERAALEYAEAMTITGQRVG
                     DELFARVRSFFDEAQIVELTAAVALENFRSKLNVPLGIEAQGFCVIGPRSALGD"
     gene            complement(709..951)
                     /locus_tag="VNU03_05690"
     CDS             complement(709..951)
                     /locus_tag="VNU03_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP73983.1"
                     /translation="MPRVREIEDDAGDPTLHELFERERGVFGALLNTTKVLAHCPPIL
                     KAAKQLGASIEKSGRLPAALLPLVYLRVALINGCPF"
     gene            complement(955..1434)
                     /locus_tag="VNU03_05695"
     CDS             complement(955..1434)
                     /locus_tag="VNU03_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP73984.1"
                     /translation="MGARAESLAKKYEAKAAELTATIQKLSDADWKKVTTAEKWPVGV
                     TAHHVAGGHEPISGIVKTIAAGQSIPNFTPGMLDEMNAKHAKEFAGCTKAETLALHEK
                     GVKAAVAVVRSLGDADLDKSGTVLVGLPAMTAQQAIEGILINHIDDHMGSIRKTVGA"
     gene            1619..>3187
                     /locus_tag="VNU03_05700"
     CDS             1619..>3187
                     /locus_tag="VNU03_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWP73985.1"
                     /translation="MTRSLLLSCEAVGKAYGTRSLFEELSFGLFEGDQVGLVGPNGAG
                     KSTLLKILADLESPDRGRRSLRGGVRVGYVPQDPAFPPGGTVEDVVASALSAVDEADR
                     PGRIAHALGRAGFADGRPEVAALSGGWQKRLAIARELAAEPDVLLMDEPTNHLDVDGI
                     LWLEALLAERARACLVVSHDRYFLEHVATRMLEIDRKYPSGLFETDGRYSEFLVRRDE
                     FLRGQAAYEESLANTVRREIEWLRRGAKARSTKAKGRIKEADRLIEELRDARTRGATR
                     TAGIDFVASGRRTRELLVARGLTKALGGRRLVRGLDLIITPGVRVGLMGPNGSGKTTL
                     LNLLAGALEPDAGSIERADGLRTVRFEQQRAWLDPDLPLRRALAPEGDAVVFQGRNVH
                     VAAWAKRFLFPPEHLEVPVGRLSGGEQARIHIARLMREPADLLILDEPTNDLDIPTLD
                     VLEESLAAFEGGLVLVTHDRFMLDRIATVIVALDGQGGAETFADYAQWEGARRERAPT
                     SKLASTSLATGPLTL"
BASE COUNT          475 a         1112 c         1115 g          485 t
ORIGIN      
        1 atccaggttc ccgctgggtc gtgcaccgcg aagcggcgct ggccccaagg ctcgtcatgc
       61 aacgggtacg cgatctcgag gccggcgccc ttcaggcgct cgaactcggc ccgggcgtcc
      121 tcgacctgaa gagtcagtag cagccctttc ccgttgaaca catcgcggcc gggtggccgg
      181 gacggatgat ccggggtcat gaacgcgagg ccaaagggat ggtccccgct ggcggcgaga
      241 tagacgaacc aggacgcctc gaacaccacc tgaaagccga accaccgaac gtagaagtcg
      301 cgacactcga cgcgcttctc ggtcaccacg atcgagtaca ggtccttgat gtgcatgccg
      361 gcgcgtgggg cggtacccgg tgggacccta gtcgcccagg gccgatctcg gtccgatcac
      421 gcagaagccc tgggcctcga tgcccagcgg aacgttgagc ttgctccgga agttctcgag
      481 cgccaccgcc gcggtcagct cgacgatctg ggcctcgtcg aagaaggacc gcacgcgcgc
      541 gaacagctcg tccccgaccc gctggccggt gatggtcatg gcctccgcgt actccagcgc
      601 ggcgcgctcc accggcgaga agcgcgcgct ctcccgccag gtcggcacct cccgcacctt
      661 ctcctcgtcc gcccccgcct gcatcccccg ggatgcgttg atgtcaattc agaaggggca
      721 gccgttgatc agcgcgaccc ggagatagac cagcggcagc agggcggcgg ggagccgccc
      781 ggacttctcg atcgacgccc cgagctgctt ggccgccttg aggatcggcg ggcagtgggc
      841 gaggaccttg gtagtgttga ggagcgcgcc gaagaccccc cgctcccgct cgaacagctc
      901 gtggagggtg gggtctccgg cgtcgtcctc gatctcgcgg acccgcggca cgggtcaggc
      961 gccgacggtc ttgcggatgc ttcccatgtg atcgtcgatg tggttgatca ggatgccttc
     1021 gatggcctgc tgggcggtca tggccggcag gccgacgagc accgtcccgc tcttgtcgag
     1081 gtcggcatcg ccgagcgagc gcaccaccgc gaccgccgcc ttgacaccct tctcgtgcag
     1141 ggcgagggtc tcggccttgg tgcagcccgc gaactccttg gcgtgcttgg cgttcatctc
     1201 gtcgagcatg cccggggtga agttggggat ggactggccg gcggcgatcg tcttcacgat
     1261 ccccgagatc ggctcgtgtc cgcccgccac atggtgcgcg gtgacgccca ccggccactt
     1321 ctcggcagtg gtcaccttct tccagtcggc gtcggagagc ttctggatgg tggcggtcag
     1381 ctcggcggcc ttggcctcgt acttcttcgc cagcgattcg gctcgtgcgc ccatgtgggt
     1441 gcctcctccg ttggggtatg ggacgtcccg aagtatacct gaggcggcaa gaccgcatat
     1501 aatcagatgg cgaaacccgc acctcgcaca caccggaagg ggggtgaagc acgtggccaa
     1561 gggacagaac atgaagaagg aaaagaaaaa gccgaagaag gagaagaagt agcgtcgcgt
     1621 gacccgatcg ctgctcctga gctgtgaagc agtcggcaag gcctacggca cccggtccct
     1681 cttcgaggag ctgtccttcg ggctcttcga gggggaccag gtgggccttg tgggtccgaa
     1741 cggggccggc aagtcgacgc tcctcaagat cctggccgac ctcgagtcgc ccgatcgcgg
     1801 gcggcgctcg ctgcgcggcg gggtgcgcgt ggggtacgtg ccgcaggatc ccgccttccc
     1861 tccgggcggc accgtggagg acgtggtggc ctccgcgctg agcgcggtcg acgaggccga
     1921 tcgtcccggc cgcatcgcgc acgcgctcgg ccgggccggc ttcgcggacg gccgcccgga
     1981 ggtggccgcg ctctccgggg gctggcagaa gcgcctcgcg atcgcccgcg agctggccgc
     2041 cgagcccgac gtcctcctga tggacgagcc caccaaccac ctggacgtcg acggcatcct
     2101 ctggctcgag gcgctcctcg ccgagcgggc gcgtgcgtgc ctggtggtga gccacgaccg
     2161 ctacttcctc gagcacgtcg ccacccgcat gctcgagatc gaccgaaagt atccctccgg
     2221 cctcttcgag accgacgggc gctacagcga gttcctcgtc cggcgcgacg agttcctccg
     2281 ggggcaggcc gcgtacgagg agtcgctcgc caacaccgtc cggcgcgaga tcgagtggct
     2341 gcggcgcggg gccaaggcgc gctccaccaa ggcgaagggt cgtatcaagg aggccgatcg
     2401 cctcatcgag gagctgcggg acgcgcggac ccggggcgcc acccggaccg ccggcatcga
     2461 cttcgtcgcc tccggccgtc gcacccgcga gctgctggtc gcccgcgggc tgaccaaggc
     2521 cctcgggggt cggcggctgg tcagggggct cgacctgatc atcaccccgg gcgtgcgcgt
     2581 gggtctcatg gggccgaacg gcagcggcaa gaccacgctg ctcaacctgc tggccggcgc
     2641 cctcgagccg gacgcgggct cgatcgagcg cgcggacggc ctccgcaccg tgcgcttcga
     2701 gcagcagcgc gcctggctcg atcccgacct gccgctgcgc cgcgcgctcg cgcccgaggg
     2761 cgacgcggtc gtgttccagg gtcgcaacgt gcacgtggcc gcgtgggcca agcgcttcct
     2821 gttcccgccg gagcacctcg aggtgccggt gggccggctc tcgggcggcg agcaggcgcg
     2881 tattcacatc gcgcgcctca tgcgcgagcc cgccgacctg ctgatcctcg acgagccgac
     2941 caacgatctg gacatcccga cgctcgacgt gctcgaggag agcctggccg cgttcgaggg
     3001 cgggctggtg ctggtgaccc acgaccggtt catgctggat cgcatcgcca ccgtcatcgt
     3061 ggccctcgac gggcagggcg gggcggagac gttcgcggac tatgcgcagt gggagggggc
     3121 gcggcgggag cgggccccga cctcgaagct cgcctccacc tccctcgcca ccggacccct
     3181 cacccta
//
LOCUS       DATMCV010000244         2413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_747323, whole genome shotgun sequence.
ACCESSION   DATMCV010000244 DATMCV010000000
VERSION     DATMCV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747323"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VNU03_05705"
     CDS             complement(<1..816)
                     /locus_tag="VNU03_05705"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP73986.1"
                     /translation="MFDAIIVGAGFAGMYMLHRLRGLGLTARVFEAGKGVGGTWYWNR
                     YPGARCDVESMDYSYSFSDELQQEWRWSERYSAQPEILRYANHVADRFDLRRDIQFET
                     RVIRAAFDEAASRWDVETDRGDRVSARFCVMATGCLSDAQVPDFEGLSSFEGKWYHTG
                     KWPHEGVDFTGRRVGMIGTGSSAIQSIPIIARQATHLYVFQRTPNYSMPAQNAPLDAE
                     YERRVKAAYADFRRQARESRVGFVTERSGDSALAVPAEEREGEYEKRWRRGGLG"
     gene            complement(816..2123)
                     /gene="larA"
                     /locus_tag="VNU03_05710"
     CDS             complement(816..2123)
                     /gene="larA"
                     /locus_tag="VNU03_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HWP73987.1"
                     /translation="MADERCPAAIPLLYGRGTLPVTPPRGCVATVIEKRAMPVLADPA
                     GAVRGALARPVGTPPLAELARGKRSACILICDITRPVPNGLFLAPLIRTLLDAGVPPA
                     GITVLVATGLHRPNEGAELAELVGDPWVLDAVTVVNHDATVDRDHVLLGRTPGRATVV
                     RLDRRLVEADLKIATGLVEPHFMAGWSGGRKVIAPGVAHAETITTFHNSAFMAHPRAA
                     NCVLDGNPLHEEQLAIVEMLGGALALNTVIDDRRRLAFVNFGEVVQSHLQAVEFARGY
                     AQVSVPRRFRTVVTSAAGYPLDKTYYQTVKGMVGPLDILEPGGELIVASACSEGLGSK
                     HYVEAQRRLVDLGPDGFAASIAGKPHADIDEWQTQMQLKPMRVGRVRLYTDGLDAVGA
                     GLTGVERVHDLSAAVAESMARQRDPRVAFVPEGPYVVPVHEEA"
BASE COUNT          335 a          883 c          866 g          329 t
ORIGIN      
        1 gccgaggccg ccccgcctcc agcgcttctc gtactcgccc tcccgctcct cggccggcac
       61 cgccagggcg gagtcgccgc tccgctcggt cacgaagccc acccgcgatt cgcgggcctg
      121 tcgccggaag tcggcgtagg ccgccttcac gcgccgctcg tactcggcgt cgaggggggc
      181 gttctgcgcg ggcatgctgt agttgggggt gcgctggaag acgtagaggt gggtggcctg
      241 ccgggcgatg atcgggatcg actggatcgc ggaggacccg gtgccgatca tcccgacgcg
      301 ccggccggtg aagtcgacgc cctcgtgcgg ccacttgccg gtgtggtacc acttgccctc
      361 gaacgaggag aggccctcga agtcgggcac ctgcgcgtcg gagaggcagc cggtcgccat
      421 cacgcagaag cgggccgaga cccggtcgcc gcggtcggtc tccacgtccc agcggctcgc
      481 cgcctcgtcg aacgccgcgc gaatcacgcg ggtctcgaac tggatgtcgc ggcgcaggtc
      541 gaagcggtcc gccacgtggt tggcgtatct caggatctcc ggctgggccg agtaccgctc
      601 gctccagcgc cactcctgct gcagctcgtc ggagaacgag taggagtagt ccatgctctc
      661 cacgtcgcag cgcgcccccg ggtaacggtt ccagtaccac gtgccgccga cgcccttgcc
      721 cgcctcgaac acccgcgcgg tgagcccgag cccccgcagc cggtggagca tgtacatccc
      781 ggcgaacccc gccccgacga tgatcgcgtc gaacatcagg cctcctcgtg caccggcacc
      841 acgtaggggc cctcgggcac gaacgccacg cggggatccc gctggcgcgc catgctctcg
      901 gccaccgcag ccgagagatc gtggacgcgc tccaccccgg tcaggcccgc gccgaccgcg
      961 tcgaggccgt cggtgtagag ccgcacgcgc ccgacccgca tcggcttgag ctgcatctgg
     1021 gtctgccact cgtcgatgtc ggcgtggggc ttgcccgcga tgctcgccgc gaagccgtcg
     1081 gggcccaggt ccaccaggcg gcgctgcgcc tcgacatagt gcttcgagcc gagcccctcc
     1141 gagcaggcgg aggccacgat cagctcgccg cccggctcca ggatgtcgag cggccccacc
     1201 atgcccttca cggtctggta gtacgtcttg tcgagcgggt agccggccgc gctcgtgacc
     1261 acggtgcgga agcggcgcgg caccgacacc tgcgcgtagc cccgcgcgaa ctccaccgcc
     1321 tggagatggc tctgcacgac ctcgccgaag ttgacgaagg cgagccgccg ccgatcgtcg
     1381 atcacggtgt tgagcgcgag cgcgccgccc agcatctcca cgatggcgag ctgctcctcg
     1441 tgcagcgggt tgccgtccag cacgcagttg gccgcgcgcg ggtgcgccat gaatgccgag
     1501 ttgtgaaagg tggtgatggt ctcggcgtgg gccaccccgg gcgcgatcac cttgcgcccg
     1561 cccgaccagc cggccatgaa gtgcggctcc accaggccgg tggcgatctt gagatcggcc
     1621 tccaccaggc gccgatcgag gcggacgacg gtggcgcggc ccggcgtgcg ccccagaagc
     1681 acgtggtcgc ggtccaccgt ggcgtcgtgg ttcaccaccg tcaccgcgtc gagcacccac
     1741 gggtcgccga ccagctcggc cagctccgcg ccctcgttcg gccggtggag gccggtggcg
     1801 accaggaccg tgatcccggc cggcggcacg cccgcgtcca gcagggtccg gatcagcgga
     1861 gccaggaaga ggccgttcgg cacggggcgg gtgatgtcgc agatcaggat gcaggccgag
     1921 cgcttgccgc gcgccagctc ggcgagcggt ggagtgccga ccggccgggc gagggcgccg
     1981 cgcaccgcgc cggcggggtc cgccagcacc ggcatcgcgc gcttctcgat gacggtcgcc
     2041 acgcagccgc gcggcggggt caccggcagc gtgccgcggc catagagcag gggaatcgcc
     2101 gccgggcagc gctcgtccgc catggccccg agtatgccat ggcggcacgg gatccggtcc
     2161 ggcggctcac cgtctgggcg cggcgcaacc ccacattggc atccgcactc ctacgagtgg
     2221 ccgtattatt cgaagggtcg ggcggcccgg ctcgaggaca agagatgact cccgaaggag
     2281 gctcggccat acgccatcgg gtccggtgcg cgaccgtggg attggccgtg gcgttgctga
     2341 ccgccgtctg cgcggcgccg cccgccaggg cccaggccgg cgcgggggga agcgagccat
     2401 cgcggcccga gta
//
LOCUS       DATMCV010000245         2346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_946992, whole genome shotgun sequence.
ACCESSION   DATMCV010000245 DATMCV010000000
VERSION     DATMCV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946992"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VNU03_05715"
     CDS             complement(<1..339)
                     /locus_tag="VNU03_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWP73988.1"
                     /translation="MMINGAPQSHEVEPRLLLVHYIRELAGLTGTHVGCDTSQCGACT
                     VHVNGVAVKSCTLLSVQCDGAQVMTIEGLAKDGKLHPIQDAFWEKHGLQCGFCTPGMV
                     MAIVSLLKENP"
     gene            complement(430..1692)
                     /gene="larC"
                     /locus_tag="VNU03_05720"
     CDS             complement(430..1692)
                     /gene="larC"
                     /locus_tag="VNU03_05720"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HWP73989.1"
                     /translation="MKSISRLAYFDCPSGASGDMILGALVDAGVPFETLERELAGLGV
                     DGYRLERSEVMKAGFRATKIDVHLAGYDRDPGYMGRVPDPGAHQPHHPAGHRGLGEIL
                     AILDRSHLAPEVRDMASRVFRRLADAEARVHGTTPERVQFHDVGAIDAIVDVTGSCIG
                     LHLLGIDAVHFGALPVGGGFIDGPHGRIPVPGPATAELLKGFPTLDTGIRRELVTPTG
                     AAILTTLATGAGAMPAMRVTAVGYGAGTMELETPNVLRVFVGEAAVAAPTGTPTETII
                     QVETTVDDMSPQLYEPLLERLLESGALDAWLTPVIMKRSRPGVVLTALCEPGRVADLS
                     RLLFEESPTIGVRWTAYQRARLEREMVRLDTAHGPVTFKVSRLDGRAITVTPEFEEIR
                     RIARDRGLAVREVLEQARTEGRRLLSSP"
     gene            complement(1760..>2346)
                     /gene="larB"
                     /locus_tag="VNU03_05725"
     CDS             complement(1760..>2346)
                     /gene="larB"
                     /locus_tag="VNU03_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWP73990.1"
                     /translation="KTPEQVVAIVERLAQHHDNVLATRAEPVVAAALVASGLPCRVHE
                     AARLVVVKPTARDGVGLIVVVSAGTSDLPVAEEAAVTAEVMGNHVERLFDCGVAGLHR
                     LVPHLDRLNEANAIVAVAGMDGALPSVLGGLVDRPVIAVPTSVGYGASFGGVAALLAM
                     MNSCAPGVSVVNIDNGYGAAAQANQINKLATKLR"
BASE COUNT          325 a          853 c          834 g          334 t
ORIGIN      
        1 cgggttctcc ttcagcagcg acacgatggc catcaccatg cccggggtac agaagccgca
       61 ctgcagcccg tgcttctccc agaaggcgtc ctggatcgga tggagcttgc cgtccttggc
      121 caggccctcg atggtcatca cctgcgcgcc gtcgcactgg accgacagca gcgtgcacga
      181 cttcaccgcc acgccgttga cgtgcaccgt gcaggctccg cactggctcg tgtcgcatcc
      241 cacgtgagtg ccggtgaggc cggccagctc gcggatgtag tgcacgagca gcagccgcgg
      301 ctccacctca tgggattggg gggcaccgtt gatcatcatc gacacggtct ttttcaccgc
      361 gctacctcct gagtgaaggg tccgaggccg ggaaagcgcc cccaaccgta gtcccggccg
      421 ggggttctgt caaggggacg agaggagccg gcgaccctcg gtgcgcgcct gctcgaggac
      481 ctcgcgcacc gcgaggcccc ggtcgcgcgc gatgcgacgg atctcctcga actcgggggt
      541 caccgtgatc gcccgcccgt cgagccggga caccttgaag gtcaccgggc cgtgcgcggt
      601 gtcgagccgg accatctcgc gctcgaggcg ggcgcgctgg taggcggtcc accgcacgcc
      661 gatggtcggg gactcctcga agagcagccg ggagaggtcg gccacccggc ccggctcgca
      721 gagggcggtc agaaccaccc cggggcggct gcgcttcatg atgaccgggg tgagccaggc
      781 gtccagggcg ccgctctcga gcaggcgctc gagcagcggc tcgtagagct ggggcgacat
      841 gtcgtccacg gtggtctcga cctggatgat ggtctcggtc ggggtcccgg tgggcgcggc
      901 caccgccgcc tccccgacga agacccggag cacgttcggg gtctcgagct ccatggtccc
      961 cgccccgtag cccaccgcgg tcacccgcat cgccggcatc gcgcccgcgc cggtggccag
     1021 cgtggtcagg atggcggccc cggtcggggt caccagctcc cggcggatcc cggtgtcgag
     1081 ggtcgggaag cccttcagca gctccgcggt ggccgggccc ggcaccggga tgcgcccgtg
     1141 cgggccgtcg atgaacccgc cgcccaccgg gagcgcgccg aagtggaccg cgtcgatgcc
     1201 gagcaggtgg aggccgatgc acgaaccggt cacgtccacg atcgcgtcga tcgcgcccac
     1261 gtcgtggaac tgcacccgct cgggcgtggt gccgtgcacc cgggcctcag cgtcggcgag
     1321 ccggcggaag acgcgggagg ccatgtcccg gacctccggc gccaggtggc tccggtcgag
     1381 gatggccagg atctcgccga gcccccggtg gcccgccggg tggtgcggct gatgcgcgcc
     1441 cgggtccggc acgcgtccca tgtagcccgg gtcgcgatcg tagccggcca ggtgcacgtc
     1501 gatcttggtg gcgcgaaagc cggccttcat gacctccgag cgctcgagcc ggtagccgtc
     1561 gacgccgagc ccggccagct cgcgctcgag ggtctcgaag gggacgcccg cgtcgaccag
     1621 cgcgcccagg atcatgtcgc cggaggcgcc gcttgggcag tcgaagtacg cgagtcttga
     1681 aatgctcttc atctgcgggg gcccagaaat ggcccccgca ctccccctgt ccgcggtcga
     1741 ctgctcgtca tttcggggcc tagcggagct tggtcgcgag cttgttgatc tggttggcct
     1801 gggcggccgc gccgtagccg ttgtcgatgt tgaccaccga cacgccggga gcgcaggagt
     1861 tcatcatggc gagcagcgcg gccacgccgc cgaaggaggc gccgtagccc acgctggtgg
     1921 gcaccgcgat gaccggccgg tccacgaggc cgcccagcac gctcggcagc gcgccgtcca
     1981 tgcccgccac cgccacgatc gcgttggcct cgttgagccg gtccagatgc gggacgagac
     2041 gatgcagccc cgccaccccg caatcgaaca gccgctcgac gtggttgccc atcacctccg
     2101 cggtcaccgc ggcctcctct gccaccggga ggtccgaggt gcccgcgctg accaccacga
     2161 tgagcccgac cccgtcgcgc gctgtgggct tcaccaccac caggcgcgcg gcctcgtgta
     2221 cgcggcacgg cagcccgctc gcgaccaggg cggcggccac cacgggctcc gcgcgggtgg
     2281 ccagcacgtt gtcgtggtgc tgggccaggc gctccacgat ggccaccacc tgctccgggg
     2341 tcttgc
//
LOCUS       DATMCV010000246         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_809749, whole genome shotgun sequence.
ACCESSION   DATMCV010000246 DATMCV010000000
VERSION     DATMCV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809749"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1932)
                     /locus_tag="VNU03_05730"
     CDS             complement(190..1932)
                     /locus_tag="VNU03_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HWP73991.1"
                     /translation="MSDAHLLIDGGIVCAYFAMITAIGLYLGRRDRSLSDFALAGRRV
                     PRWAVMASIIAAETSAATFLGVPGEGYTTRSFAYVQIIVGLILGRVVVGHVFLKPYYT
                     FKVYTVYDYLGVRFGPWTKAYVSALFLFMRTLASGTRLFIPSLLMVLAYRLFVSGTAP
                     RFGDEGVTTVGPYLVAIVALTVVTCLYTAVGGIKAVIWTDVIQAALMFGGALVAIGTL
                     LAHVGGLSAVLQAVPQLATHEGYFLHGFEADRVAQWREAHHLTAMGVWDYVKLVLASD
                     YTLFSAIVGATLGNMAAFGTDQDMVQRMLTAETHQKARRSLITAAFMDLPVAGAFVFI
                     GILLFVYYQQDPTFRPRATADVFVSYILNVMPVGVRGFVVAGVFATAMGSFSAALNAL
                     ATSATNDWYIRWVPGRSETHYVRAARWFTVLFAGLMVVIAGLFAYAKVTSPDVRIIPV
                     VLGIAGFILGPMLGVFLVGMFTTGRGSDRGNMLAITAGLLATVVAGKLHLTILNGLAP
                     WLGLAGTLQQPAWIPEVAFTWWAMIGAVVVFAVGVLFRTPEAVRTALARHARQAQLAE
                     TVPLALRDQGRGPA"
BASE COUNT          352 a          669 c          798 g          261 t
ORIGIN      
        1 ccactcggtc tgctcgacgg gcaaccggta gaggaactgg ctcgacattg gccctctcct
       61 tcgtagcttt ggcggtggct ttggggcgtg acaccccggc gtgcccccgg gggtgaatcg
      121 gttgcggaag gatagcactg aacggcggtt caatggaacc ccccgcgccg cagcgcggcg
      181 gacgacgagt caggccgggc cgcggccctg gtcccgcagg gcgagcggga ccgtctcggc
      241 gagctgggcc tggcgcgcgt ggcgggcgag cgcggtccgc accgcctcgg gggtgcggaa
      301 caggacgccg accgcgaaca cgaccacggc gccgatcatc gcccaccagg tgaaggcgac
      361 ctcggggatc caggcgggct gctggagggt gccggccagg ccgagccacg gggcgagccc
      421 gttcaggatc gtgaggtgga gcttgccggc caccacggtg gcgaggaggc cggcggtgat
      481 ggcgagcatg ttgccgcggt ccgagccgcg gccggtggtg aacatcccga ccaggaagac
      541 cccgagcatg gggcccagga tgaagcccgc gatgccgagc accaccggga tgatccgcac
      601 gtcggggctc gtcaccttgg cgtaggcgaa gaggcccgcg atcacgacca tcaggcccgc
      661 gaagagcacc gtgaaccagc gggcggcccg cacgtagtgg gtctcgctcc ggccgggcac
      721 ccagcggatg taccagtcgt tggtggcgct ggtggcgagc gcgttcagcg cggccgagaa
      781 cgagcccatc gcggtggcga agacgccggc caccacgaag ccgcgcaccc cgaccggcat
      841 gacgttcagg atgtaggaga cgaagacgtc ggcggtggcg cggggccgga aggtcggatc
      901 ttgctggtag tagacgaaga gcaggatgcc gatgaacacg aaggcccccg ccaccggcag
      961 gtccatgaag gcggcggtga tcaggctgcg gcgcgccttc tggtgggtct ccgcggtcag
     1021 catgcgctgg accatgtcct ggtcggtgcc gaacgcggcc atgttgccga gcgtcgcccc
     1081 cacgatggcc gagaagagcg tgtagtcgct ggccaggacc agcttgacgt agtcccacac
     1141 gcccatcgcg gtgaggtggt gggcctcccg ccactgcgcg acgcggtccg cctcgaagcc
     1201 gtggaggaag tagccctcgt gcgtggccag ctgcgggacg gcctgcagca cggcggagag
     1261 cccgcccacg tgggccagca gggtgccgat cgcgacgagc gcgccgccga acatcagggc
     1321 cgcctggatc acgtcggtcc agatgaccgc cttgatcccg cccaccgcgg tgtagaggca
     1381 ggtgaccacc gtgagcgcga cgatcgccac caggtagggg ccgacggtgg tgacgccctc
     1441 gtcgccgaac cggggcgcgg tgccggagac gaagaggcga taggccagca ccatgagcag
     1501 cgaggggatg aagagccgcg tgccggaggc gagggtgcgc atgaagagga agagggccga
     1561 gacgtaggcc ttggtccacg ggccgaagcg caccccgagg tagtcgtaga cggtgtagac
     1621 cttgaacgtg tagtagggct tgaggaagac gtggccgacc accacgcggc cgaggatcag
     1681 gccgacgatg atctgcacgt aggcgaagct gcgggtcgtg tagccctcgc cgggcacgcc
     1741 caggaaggtg gccgcgctgg tctccgccgc gatgatcgag gccatcaccg cccaccgcgg
     1801 cacgcggcgg cccgcgagcg cgaagtcgct caggctccgg tcgcgccgcc cgagatagag
     1861 gccgatggcg gtgatcatcg cgaagtaggc gcagacgatg ccgccgtcga tcagcaggtg
     1921 cgcgtcgctc atcgggccgg gcatggccca ttatcgcgga cgcggcaccg ggagccgcca
     1981 atctgcggcg gccacgcttg ccgccggtcg cggcgccggt ctatgatcgg caaccgggac
     2041 cacgagatga cgaggctttc ggatcggcgc cggggagtgg
//
LOCUS       DATMCV010000247         3133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_80892, whole genome shotgun sequence.
ACCESSION   DATMCV010000247 DATMCV010000000
VERSION     DATMCV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80892"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2202)
                     /locus_tag="VNU03_05735"
     CDS             complement(<1..2202)
                     /locus_tag="VNU03_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP73992.1"
                     /translation="MNLSRPFILRPVATTLLMAALLLAGGLAYRLLPVSALPQVDYPT
                     IQIFTFYPGASPDVMASSVTAPLERFLGQMPGLNQMTSTSSSGSSVITLQFTLSLGLD
                     VAEQEVQAAINSALTLLPPDLPNPPVYSKVNPADTPILTLGLTSATLPLPQVEDLADT
                     RLAQKIAQLPGVGLVSISGGQRPAVRVQANPAALAAYGLTLEDLRTAVATANVNQAKG
                     SFDGPRQSSVIGANDQLLTSRDYKPLIVAFRNNAPVTLENVADVIDDAENVRQAAWMN
                     GTPAVILNIQRQPGANVIAVVDRIHRLLPQLRASLPAAVEVSVLTDRTVTIRGSVTDV
                     QFELMLSVALVVLVIFLFLRTVPATLIPAVAVPLSLVGTFGVMVLLGFSLNNLSLMAL
                     TISTGFVVDDAIVMIENIARYIEAGDPPRQAALKGAAQIGFTILSLTVSLIAVLIPLL
                     FMADVVGRLFREFAITLGITILLSAVVSLTLTPMMCARLLRHTPPERQGRLYRWSERA
                     FDRIIAAYGTSLRWVLGHQPATLAVAVVTLGLTLVLYVVVPKGFFPVQDTGVIQGISE
                     ASQTISFPAMAERQQALAAVILTDPAVESLSSFIGVDGTNVSVNSGRILINLKPLARR
                     GGVSATDVIHRLKPALARVDGIQLFMQPVQDLTVEDRVSRTQYQYSLEDPDGRELNLW
                     APRLVDALRARRELADVSTDQQDQGLGTALVVDRRTASRFGIGPQAIDNTLY"
     gene            complement(2199..>3133)
                     /locus_tag="VNU03_05740"
     CDS             complement(2199..>3133)
                     /locus_tag="VNU03_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HWP73993.1"
                     /translation="LNTVTVRSRVDGELMAVNFREGQLVNRGDLLAEIDPRPFQAQLT
                     QFEGQLERDQALLSNARLDLGRFEALVKTDAVPRQQLDTQASLVQQLEGTVKNDQGQI
                     EATKVQLIYCRITSPITGRVGLRLVDPGNIVHATDTGGLLIVTQLQPITVIFTIPEDS
                     IPTVLDRLRSGVRLPVEAYDREVRTKLAEGALLTIDNQVDPTTGTVRLRAQFPNTDNR
                     LFPSQFVNARLRLETRRGATVVPTAAIQSSPKGPFVYVVRPDRTVTVRPVGVGAADGD
                     DVSIERGLAVGEQVVVEGAERLRDGAAIALRNGP"
BASE COUNT          490 a         1024 c         1155 g          464 t
ORIGIN      
        1 gtagagggtg ttgtcgatcg cctgaggacc tatgccaaag cgcgaggcgg tgcggcggtc
       61 gaccaccagc gcggtgccga gtccctgatc ctgctggtcg gtgctgacgt cggccagctc
      121 gcgccgcgcg cgcagcgcgt cgaccaggcg gggcgcccag aggttgagct cgcgcccatc
      181 cggatcctcg aggctgtact ggtactgcgt gcggctcacc cggtcctcca cggtgagatc
      241 ctgcaccggc tgcatgaaga gctggatgcc gtcgacccga gccagcgcgg gcttcaggcg
      301 gtggatgacg tccgtcgcgc tcaccccgcc gcgccgggcg agcggcttca ggttgatgag
      361 gatgcgcccg ctgttgaccg agacgttggt gccgtccacg ccgatgaagg aggacaggct
      421 ctcgaccgct ggatcggtga ggatcaccgc ggccagggcc tgctgccgct cggccatcgc
      481 cgggaacgag atcgtctgcg acgcttccga gatgccctgg atgaccccgg tgtcctggac
      541 cgggaagaac cccttcggca ccaccacgta gagcaccagc gtgagcccga gggtgacgac
      601 cgccaccgcg agggtcgccg gctggtggcc gagcacccag cgcaggctcg tgccgtaggc
      661 cgcgatgatg cggtcgaagg cgcgctcgga ccaccggtag agccggccct ggcgctcggg
      721 aggcgtgtgg cgcagcagcc gggcgcacat catcggggtc agggtcagcg acaccaccgc
      781 ggagaggagg atcgtgatcc cgagggtgat cgcgaactca cggaacagcc ggcccaccac
      841 gtcggccatg aacaggagcg ggatgagcac cgcgatcagc gagacggtga gggacaggat
      901 ggtgaagccg atctgggcgg cgcccttgag cgcggcctgt cgcggcgggt cgcccgcctc
      961 gatatagcgc gcgatgttct cgatcatgac gatggcatcg tccaccacga acccggtgga
     1021 gatggtgagc gccatcagcg agagattgtt caggctgaac ccgaggagca ccatcacgcc
     1081 gaaggtccct accagcgaga gggggaccgc gaccgccggg atgagggtgg cgggaacggt
     1141 gcgcaggaag aggaagatga ccagcacgac cagcgccacc gacagcatca gctcgaactg
     1201 gacatccgtg accgatccgc gaatcgtcac ggtgcggtcg gtgagcacgg aaacctcgac
     1261 cgcggccggc agggaggccc gcagctgggg caggagccgg tggatgcggt ccacgaccgc
     1321 gatcacgttg gcgccgggct gccgctggat gttcaggatg accgccgggg tgccgttcat
     1381 ccaggccgcc tggcgcacgt tctcggcgtc gtcgatcacg tcggccacgt tttcgagggt
     1441 gaccggggca ttgttgcgga acgcgacgat cagcggcttg tagtcccggc tcgtcaggag
     1501 ctggtcgttg gccccgatga ccgacgactg gcgcggcccg tcgaagctgc ccttggcctg
     1561 gttgacgttc gcggtcgcca ccgcggtacg caaatcttcc agggtgaggc cgtacgccgc
     1621 gagcgcggcc gggttggcct gcacccgcac cgcgggccgc tgcccgccgc tgatgctgac
     1681 gagcccgacg ccgggaagct gggcgatctt ctgggcgagc cgcgtatcgg ccaggtcctc
     1741 gacctggggt agcggcaagg tcgccgaggt cagcccgagg gtgaggatgg gcgtatcggc
     1801 cgggttcacc ttgctgtaca cgggcgggtt cgggagatcc ggcggcagga gcgtcagcgc
     1861 cgagttgatc gccgcctgca cctcctgctc ggccacgtcg agacccaggc tcagggtgaa
     1921 ctgcagcgtg atgaccgagc tcccactcga gctggtggac gtcatctggt tcaggccggg
     1981 catctgcccg aggaaccgct cgagcggggc ggtgaccgac gacgccatca cgtcggggct
     2041 cgcccctggg tagaacgtga agatctggat ggtcgggtag tccacctggg gcaaggcgga
     2101 gaccggcagc aggcggtagg cgaggccgcc cgccagcagg agggcggcca tgagcagggt
     2161 cgtcgcgacc ggccgcagga tgaacgggcg cgacaggttc acgggccgtt ccgcagcgcg
     2221 atggccgctc cgtcgcgaag gcgctcggcg ccttcgacca cgacctgctc ccccaccgcc
     2281 agaccccgct cgatcgacac gtcgtcacca tccgccgccc cgacgccgac cgggcggacc
     2341 gtgaccgtcc ggtcgggccg caccacgtag acgaacggcc ccttcgggct cgactggatg
     2401 gccgcggtcg gcaccaccgt cgcgcctcga cgggtctcga gccggagacg ggcattgacg
     2461 aactggctgg ggaacaaccg gttgtcggtg ttcgggaact gggcccggag gcggaccgtg
     2521 ccggtggtgg gatcgacctg gttgtcgatg gtgagcagcg cgccctccgc gagcttggtc
     2581 cgcacctccc ggtcgtaggc ctccaccggc aggcggacgc cgctccggag ccggtccagc
     2641 acggtgggaa tgctgtcctc ggggatggtg aagatcacgg tgatcggctg gagctgggtg
     2701 acgatgagga ggccgccggt gtcggtcgca tgcacgatgt tgccgggatc caccagccgg
     2761 agccccacgc gccccgtgat gggtgacgtg atgcgacagt agatgagctg gaccttcgtg
     2821 gcctcgatct ggccctggtc gttcttgacc gtgccctcga gctgctggac gagcgaggcc
     2881 tgggtgtcca gctgctgccg cggcaccgcg tccgtcttga cgagcgcctc gaaccgcccg
     2941 agatccaggc gggcgttgct gagcagtgcc tggtcgcgct cgagctgccc ctcgaactgg
     3001 gtgagctgcg cctggaacgg gcgcgggtcg atctcggcga gcaggtcccc gcgattcacg
     3061 agctggccct cgcggaagtt gaccgccatc agctcgccgt cgacccggct tcgcaccgtg
     3121 accgtgttca gcg
//
LOCUS       DATMCV010000248         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_212417, whole genome shotgun sequence.
ACCESSION   DATMCV010000248 DATMCV010000000
VERSION     DATMCV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212417"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VNU03_05745"
     CDS             <1..801
                     /locus_tag="VNU03_05745"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWP73994.1"
                     /translation="IDSSHISFLHAPIDHRNSEVTQDMDRVSFGVGAAVSTGDRAPRF
                     EVVDTDYGVLIGARRANPDGRWYWRVTQFLLPFYTMPPTDLDERVVQSHIWVPMDDSH
                     VVNWMVTWHPDRKLTPEEIQLHVEGKGAHVCDYAPATSEPYGDVRTAANRENDYFMDW
                     EVHRSRMYCGIPGFGVQDQAVQESQGDIVDRSQERLGSSDTAIIQVRRRMMTAARALR
                     EHGTPAPGANPRSFLVRSTSVVLAPGESWVDGARARVLVRAGDQLTLV"
     gene            825..1589
                     /locus_tag="VNU03_05750"
     CDS             825..1589
                     /locus_tag="VNU03_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP73995.1"
                     /translation="MAGRVALVTGGGGGIGEATGRLFAEEGAAVALLDADGDAVARAA
                     EAIRTAVPGASLLPLVGDVTREAEAARIVEETAARLGGLHTLVNVAGVRVYAPLAEAD
                     AADWERIIGVNVLGTAHCCKAALPRMRSTGRGTIVNVSSVYGLMGRTGMGQYDTTKAA
                     VLGFTRALAVEEAPHRVRVNAVCPGGTITPYHIRRAAARGVSETALRGERTRDNLLGR
                     WAEPREVAYPILFLACDESSFVTGATLMVDAGRSIL"
     gene            1640..>2174
                     /locus_tag="VNU03_05755"
     CDS             1640..>2174
                     /locus_tag="VNU03_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HWP73996.1"
                     /translation="MDVLRGLTVAAMVLVNNPGSWASVYWPLRHAEWSGFTPTDMIFP
                     FFLFIVGVSIPLALGPRLEERQAAGLVGRVLWRSFAIFALGLLLHALPWFHFATLRIP
                     GVLQRIAVCYLLAALLVIASSGGAGWRLQAVVAGGLLVGYWLLMTRLPPPGGVAGDLS
                     PAGNLAGYVDRVVLGPHL"
BASE COUNT          264 a          792 c          800 g          318 t
ORIGIN      
        1 atcgactcga gccacatctc gttcctccac gcgccgatcg accaccgcaa cagcgaggtc
       61 acccaggaca tggaccgggt gagcttcggg gtcggggcgg ccgtctccac cggtgaccgc
      121 gccccgcgct tcgaggtggt cgacaccgac tacggcgtcc tgatcggcgc ccgccgcgcc
      181 aacccggacg gccgctggta ctggcgggtc acccagttcc tcctgccgtt ctacacgatg
      241 ccgcccaccg atctcgacga gcgcgtcgtg cagtcgcaca tctgggtgcc gatggacgac
      301 tcccacgtgg tgaactggat ggtgacgtgg cacccggatc gcaagctcac tcccgaggag
      361 atccagctcc acgtcgaggg caagggcgcc cacgtctgcg actacgcccc ggccacctcc
      421 gagccctacg gcgacgtgcg caccgcggcc aatcgagaga acgactactt catggactgg
      481 gaggtccacc gcagccggat gtactgcggc atccccggct tcggggtgca ggaccaggcg
      541 gtgcaggaga gccagggcga tatcgtcgac cgcagccagg agcgcctcgg cagcagcgac
      601 accgcgatca tccaggtgcg ccggcgcatg atgacggccg cccgcgccct ccgcgagcac
      661 ggcacgcccg cccccggcgc gaacccgcgc tccttcctgg tgcgctccac ctccgtggtg
      721 ctggccccgg gcgagagctg ggtggacggc gcccgggcgc gagtgctggt gcgggcgggg
      781 gaccagctca cgctcgtctg acgcaggccc cgatgcccgg gcgcatggcc ggccgcgtcg
      841 cgctcgtcac cggcggtggt ggcggcatcg gcgaggcgac cgggcgactc ttcgcggagg
      901 agggcgcggc ggtggccctg ctcgatgccg acggggacgc cgtcgcccga gcggccgagg
      961 ccatccggac cgcggtgccg ggcgcctcgc tgctgccgct cgtcggcgac gtcacccgcg
     1021 aggcggaggc ggcccgcatc gtggaggaga ccgcggcgcg cctcggcggg ctccacaccc
     1081 tggtgaacgt ggccggggtg cgggtgtacg cgccgctggc cgaggccgac gccgcggact
     1141 gggagcggat catcggcgtc aacgtgctcg gcaccgccca ctgctgcaag gcggcgctgc
     1201 cgcggatgcg gagcaccggg cgcgggacca tcgtcaacgt gtcgtcggtg tacgggctga
     1261 tgggacgcac cggcatgggc cagtacgaca ccaccaaggc cgcggtgctc ggcttcaccc
     1321 gggcgctcgc ggtcgaggag gcgccgcacc gggtgcgggt caacgcggtg tgcccgggcg
     1381 ggaccatcac gccgtaccac atccgccggg ccgccgcgcg gggtgtctcg gagaccgcgc
     1441 tgcgcgggga gcggacccgg gacaacctgc tgggccgctg ggccgagccg cgcgaggtgg
     1501 cgtatcccat cctgttcctg gcctgcgacg agtcctcgtt cgtgaccggc gccacgctca
     1561 tggtggacgc gggccgatcg atcctgtgac cgcgccgccg ggcgccccgc ctcgggtcgt
     1621 ctcgtcccgg gtcatctcag tggacgtgct ccgcggtctc acggtggccg cgatggtgct
     1681 ggtgaacaat cccggctcgt gggcgtccgt ctactggccc ctccggcacg cggagtggag
     1741 cgggttcacc ccgacggaca tgatcttccc gttcttcctc ttcatcgtcg gcgtgtcgat
     1801 cccgttggcg ctcgggcccc ggctggagga gcggcaggcc gcggggctgg tgggccgcgt
     1861 gctgtggcga tccttcgcga tcttcgctct cgggctcttg ctgcacgcgc tgccctggtt
     1921 ccacttcgcc accctccgca tccccggcgt gctccagcgg atcgcggtct gctacctgct
     1981 cgccgcgctg ctggtgatcg cgagcagcgg cggcgcgggc tggcgtctgc aggccgtcgt
     2041 ggcgggcggg ctgctcgtcg gctactggct gctcatgacg cggctgcctc ctccgggcgg
     2101 ggtggccggg gacctgagcc cggcgggcaa cctggccggc tacgtcgacc gcgtggtgct
     2161 cgggccgcac ctct
//
LOCUS       DATMCV010000249         6709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_747466, whole genome shotgun sequence.
ACCESSION   DATMCV010000249 DATMCV010000000
VERSION     DATMCV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747466"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VNU03_05760"
     CDS             complement(<1..1058)
                     /locus_tag="VNU03_05760"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387596.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HWP73997.1"
                     /translation="MAVSPLAGRPAPASMLIDPARLEREYYARRPDVDDPTQLVAFGT
                     SGHRGSPLRGSFTEGHILAITQAICDYRRGQGIDGPLYMGKDTHAVSGPAERTALEVL
                     AANGVEVVIQRDDGVTPTPVVSHAILVYNHGRTAHVADGIVVTPSHNPPEDGGFKYNP
                     PNGGPADTDVTGWVERRANQLLREGLTGVKRVPVEAAMKAATTHPEDLVTPYVADLGS
                     VVDLDAVRAAGLRIAVDPLGGASQPYWEPISALYGLDITVVNPVIDPTFAFMTVDHDG
                     KIRMDCSSPYAMARLVGLRDRYRLAFANDPDADRHGIVTPAAGLMNPNHYLAVAVDYL
                     LSHRPRWSTAAAVGKTLVS"
     gene            complement(1063..3543)
                     /locus_tag="VNU03_05765"
     CDS             complement(1063..3543)
                     /locus_tag="VNU03_05765"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWP73998.1"
                     /translation="MRVVRRGLGWALGLTLAVVTAVHAQSGEPPADTGGGPIPLAEVA
                     ARAAEIRSLLETQDTAITPSREVQAVLDRLPALTDELRVRLDQTATRLGTNPPLPVVE
                     QLGSMWEATRARLKSWNETVTQWTTRVEQERGRLASLRESWTRSREAAAVSGAPKVVL
                     DQIDGVVSGLNAARGRLESRLSALLATQYRLALLQRRCDEGLSRVAQVRADRIRRLGE
                     RDGPPLWSGVLWKGAMSQTRAAAGNVLEAEQAVVGEVAREHATRIPLHALVFAVLVAA
                     LWRGRRAARRWVADDPSAASVAEVWSRPISSALVLACFAAPWMYPEATPLLGRVVALI
                     AIVPVLRLIRPSLDRVLAAALYVFGVLFLVDAVRSVLSTAPLFEHMLFLAEMLVASLV
                     MIRLLTSARRAAPLPEPGAPAEADTRAEADAPAEADTDVMAAAARRLVGRLLLAAFAG
                     AFALAGTGYVELGRLVGAGALGSSYYGLAILAAVQVARGLTGVLLRSRIFALLASVQQ
                     NRAVIERRISRVLRWAGVIAWIAATLDALTLLSAAVRGTRAALAAQWGWGAIRISLGD
                     MVVFGLTIWLAFVAAAALRLVLAEDVFPRVRMARGVPLALSAVIQYVVIFGGFTLALA
                     ALGVDLTKLTILASAFGVGAGLGLQTVVNNFASGLILLFERPIHLGDVVQVAGIGGEV
                     RHIGVRATLVRTADGAEVFVPNSLLIAQSVTNWTYSDMRRRIVLPVRVAYGATPQRVT
                     ELLTAIGARHPQVSAEPAAAAVFMGFGENSLDFELRAWTDRFGDAETIQSQLADSVYA
                     ALNEARIDLPVPRRDVRLRQVAAEPGKD"
     gene            complement(3549..4535)
                     /locus_tag="VNU03_05770"
     CDS             complement(3549..4535)
                     /locus_tag="VNU03_05770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP73999.1"
                     /translation="MPTDIALTGLAMPLAEFPAIAREAEGRGYRTAWVGEASGAEAIV
                     LSTLIATHTAKIGIANGVIPVQTRTPIVYGQAAATLAHLAPGRFGLGLGLSSEIIVGQ
                     WHGLPFAPSIQQMREAVQIIRTVAAGERVTFEGKFYRMKNFRLAIPAPAPPPRIYLAA
                     LGPRMCELGGEIADGVLLNWIPPEAVGASLAHVETGAKRAGRRLADVDIAVYVRTCVT
                     DEPAPARDTLARDITGYAIVNAYARFFEECGYAPEVAAVNRAWKAGDRAGAVKGISER
                     VLDGLGAVGPADHCREQIAAFARPGVTPVVLPFAPPGPAARASMLTTFRTFP"
     gene            complement(4535..5158)
                     /locus_tag="VNU03_05775"
     CDS             complement(4535..5158)
                     /locus_tag="VNU03_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74000.1"
                     /translation="MAPRGLFLVRMDVAHDHEALFNEVYDKEHVPLLQAVPGVIRASR
                     YRQSSPTEPRYVAAYELESLAVLQSPEWKAAGEAGRWPKDIRPHTMNRHLAAYEWVGS
                     SPGLTYRSPYVFWVMMDIEPHREALFNELYDVEHVPLLLKLPGCVNVARYRTETAGEP
                     RYLAAYEVERTDLPMSKLWNDTSDIGRWKPEVRPYTYNKRYIVSERL"
     gene            5300..5860
                     /locus_tag="VNU03_05780"
     CDS             5300..5860
                     /locus_tag="VNU03_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74001.1"
                     /translation="MPPVDPNEVLMTGENSFIRLSPDGGKTLSDRASHWRVLWCPAGG
                     GHVLFLQSTLTDGRVRIYSDNIAVARWLQTTIEKLLFPAFADTSLPVVAARFDRHGGP
                     WSTATDARVTMSWYDCIEPFVLNAPPGFNDRPIGVFSTFFPARAAQLEVNGKVASGQV
                     WAEMRGDRQGSSATLAWSETWVKPRG"
     gene            5872..>6709
                     /locus_tag="VNU03_05785"
     CDS             5872..>6709
                     /locus_tag="VNU03_05785"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWP74002.1"
                     /translation="MSGRLDGKVAIVTGGGSGIGRASALTFLREGARVVIGDLNDASL
                     AETVALAKAQGGADRLAALRADVSAERDVAALVALGVERFGGLDCLFNNAGVGGAFGP
                     IGETTVEEWDFTFAVLVRGVFLGIKHGATRMKAQGRGGSIVNTASIAGIGGGAGPHAY
                     SAAKAAVANLSRSVSAELAAHRIRVNAIAPGMIKTPLATGRREEAWDRLAREKQPWPD
                     LGLPEHIADAALFLASDDSRFITGQVLVVDGGLTAMGPDLFGHEAGSRMLRKAGLNTG
                     STG"
BASE COUNT          946 a         2478 c         2415 g          870 t
ORIGIN      
        1 gagacgaggg tcttgcccac cgcggcggcg gtcgaccacc gcggccgatg gctcagcagg
       61 tagtcgaccg ccaccgccag gtagtggttc gggttcatca gccccgccgc gggcgtgacg
      121 ataccgtggc ggtccgcgtc gggatcgttg gcgaaggcca ggcgataccg gtcccggagg
      181 ccgacgagtc gcgccatcgc gtaggggctc gagcagtcca tgcggatctt gccgtcgtga
      241 tccacggtca tgaacgcgaa ggtgggatcg atgaccgggt tgaccaccgt gatgtcgagc
      301 ccgtagagcg cgctgatcgg ctcccagtag ggctgcgacg cgccgccgag cggatccacc
      361 gcgatccgca gcccggcggc ccgcaccgcg tccaggtcca ccacgctccc gagatcggcc
      421 acgtagggcg tcaccagatc ttccggatgc gtggtcgccg ccttcatcgc ggcctcgacc
      481 ggcacccgct tcaccccggt gagcccctcg cgcaggagct ggttggcgcg ccgctccacc
      541 cagcccgtca cgtcggtgtc ggccggcccg ccgttgggag ggttgtactt gaaacccccg
      601 tcctcgggcg ggttgtgaga cggcgtgacc acgatgccgt cggccacgtg cgcggtgcgc
      661 ccgtggttgt agaccaggat cgcgtgggag acgaccggtg tcggggtgac cccgtcgtcg
      721 cgctggatga ccacctcgac gccgttggcc gcgagcacct cgagggcggt ccgctcggcg
      781 gggcccgaca ccgcgtgggt gtccttgccc atgtagagcg gtccgtcgat cccctgcccc
      841 cgccggtagt cgcagatcgc ctgggtgatg gcgaggatgt ggccttcggt gaacgagccg
      901 cgcagcggcg agccccggtg cccgctggtg ccgaaggcga cgagctgggt cgggtcgtcc
      961 acgtcgggcc ggcgcgcgta gtactcgcgc tcgagccggg ccgggtcgat cagcatcgac
     1021 gcgggcgcgg gccggccggc gagaggggag acggccatgg cctcagtcct ttccgggctc
     1081 ggccgcgacc tgccgcagcc gcacgtcgcg ccgcggcacc ggcaggtcga tccgggcctc
     1141 gttcagcgcg gcgtagacgg aatccgcgag ctggctctgg atcgtctccg cgtcgccgaa
     1201 gcgatcggtc cacgcccgca gctcgaagtc gagcgagttc tccccgaagc ccatgaagac
     1261 cgcggcggcg gccggctccg cgctgacctg gggatgccgc gccccgatcg cggtcagcag
     1321 ctcggtgacg cgctggggcg tggccccgta ggcgacccgc accggcagca cgatgcgccg
     1381 ccgcatgtcc gagtaggtcc agttggtgac cgactgcgcg atcaggaggg agttgggcac
     1441 gaacacctcc gcgccgtccg cggtccgcac cagggtcgcg cgcaccccga tgtgacgcac
     1501 ctcgcccccg atccccgcga cctgcaccac gtcgcccagg tggatcgggc gctcgaataa
     1561 caaaatgagc ccggaggcga agttgttcac caccgtctgc agcccgagcc cggcgccgac
     1621 cccgaacgcg ctcgccagga tcgtgagctt ggtgaggtcc accccgagcg cggcgagcgc
     1681 gagggtgaag cccccgaaga tgaccacgta ctggatcacc gccgacagcg cgagcggcac
     1741 cccgcgcgcc atgcgcaccc gggggaacac gtcctcggcg agcacgagac ggagcgcggc
     1801 ggcggccacg aaggcgagcc agatcgtgag cccgaagacc accatgtcgc cgagcgagat
     1861 gcggatcgcg ccccagcccc actgcgcggc cagcgcggcg cgggtccccc tcaccgccgc
     1921 ggacaggagg gtgagcgcgt ccagggtggc cgcgatccac gcgatcacgc cggcccaccg
     1981 gagcacccgg ctgatgcgac gctcgatcac cgcgcggttc tgctgcaccg aggccaggag
     2041 cgcgaagatc cggctccgca gcagcacccc ggtgagcccc cgggccacct gcaccgcggc
     2101 caggatcgcc aggccgtagt agctgctgcc gagcgccccg gccccgacca ggcgccccag
     2161 ctccacgtag ccagtcccgg ccagcgcgaa ggcccccgcg aacgcggcga gcagcagccg
     2221 gccgacgagc cgccgcgccg ccgccgccat gacatctgta tcagcctcgg cgggcgcatc
     2281 cgcctctgcg cgcgtatcgg cctcggcggg cgcgccgggc tcggggagcg gggcagcccg
     2341 gcgggccgag gtcaggagcc ggatcatcac gaggctcgca accagcatct cggcgaggaa
     2401 gagcatgtgc tcgaagaggg gcgcggtcga gagcaccgac cggaccgcgt cgaccaggaa
     2461 gagcactccg aagacgtaga gcgcggcggc caggacgcgg tcgagcgagg gccggatgag
     2521 gcgcagcacc ggcacgatcg cgatcagcgc caccacgcgg ccgagcagcg gcgtggcctc
     2581 gggatacatc cagggggccg cgaagcaggc cagcaccagg gccgaggaga tgggccggct
     2641 ccagacctcg gccaccgagg cggcgctcgg gtcgtccgcg acccagcggc gcgcggcgcg
     2701 acggccacgc cagagcgccg cgaccagcac cgcgaagacg agcgcgtgga gcgggatccg
     2761 ggtcgcgtgc tcccgggcga cctcgcccac caccgcctgc tcggcctcga gcacgttccc
     2821 cgccgccgcg cgcgtctggc tcatcgcccc cttccacagc accccgctcc agagcggcgg
     2881 gccgtcccgc tcaccgagcc gccggatccg gtccgcccgc acctgcgcca cacgcgagag
     2941 cccctcgtcg cagcgccgct ggagcagggc caggcggtac tgggtggcga gcagcgcgga
     3001 gaggcgtgac tccagccggc cgcgcgccgc attgagcccg ctcacgaccc cgtcgatctg
     3061 gtcgaggacg accttgggcg cgcccgacac cgcggccgcc tcccgcgagc gtgtccagct
     3121 ctcgcgcagg ctcgcgagcc gcccgcgctc ctgctcgacg cgggtcgtcc actgggtcac
     3181 ggtctcgttc cagctcttga gcctcgcgcg ggtcgcctcc cacatggagc cgagctgctc
     3241 gacgaccggc agcggcggat tcgtcccgag acgcgtggcg gtctggtcga gccggacgcg
     3301 cagctcgtcg gtcagtgcgg gcaagcgatc caggaccgcc tggacctccc ggctcggcgt
     3361 gatcgcggtg tcctgcgtct cgagcaggct ccggatctcg gcggcgcgcg ccgcgacctc
     3421 ggcgagcggg atcgggccgc caccggtgtc cgcgggcggc tcacccgact gcgcgtgcac
     3481 agcggtgacc acggccaggg tgaggccgag ggcccagccg agtcctcgcc ggacgacgcg
     3541 catgtcgatc aggggaaggt gcgaaacgtc gtcagcatgg aggcgcgcgc ggccgggccc
     3601 ggcggcgcga acggcaacac caccggcgtg accccggggc gggcgaaggc cgcgatctgc
     3661 tcccggcagt ggtcggcggg gcccaccgcg cccaggccgt ccagcacgcg ctcggagatc
     3721 cccttgaccg cacccgcccg atcgccggcc ttccacgcgc ggttcaccgc cgccacctcg
     3781 ggcgcgtagc cgcactcctc gaagaagcgg gcgtaggcgt tcacgatggc gtagccggtg
     3841 atgtcgcggg ccagcgtgtc ccgcgccggc gcgggctcgt cggtgacgca cgtgcgcacg
     3901 tagaccgcga tgtcgacgtc ggccagccgc cgtcccgcgc gcttggcgcc ggtctcgacg
     3961 tgagcgagcg atgcccccac cgcctccggc gggatccagt tgagcaggac gccgtcggcg
     4021 atctcgccgc ccagctcgca catgcggggg cccagcgcgg ccaggtagat gcgcggcggc
     4081 ggcgccggcg cggggatggc caggcggaag ttcttcatcc ggtagaactt cccctcgaag
     4141 gtcacccgct cacccgccgc caccgtgcgg atgatctgca ccgcctcgcg catctgctgg
     4201 atcgacggcg cgaacggcag cccgtgccac tgcccgacga tgatctcgct cgagagcccg
     4261 aggccgagcc cgaagcgccc gggggccagg tgcgccaggg tcgccgcggc ctggccgtac
     4321 acgatcgggg tgcgggtctg caccgggatc accccgttgg cgattccgat cttcgcggtg
     4381 tgggtggcga tcagggtcga gagcacgatg gcctcggcgc cggaggcctc gcccacccag
     4441 gcggtgcggt agccgcgtcc ttccgcctcg cgcgcgatgg cggggaactc ggccaggggc
     4501 atcgcgagcc cggtcagcgc gatgtcggtc ggcatcagag gcgctcgctc acgatgtacc
     4561 gcttgttgta ggtgtacggg cgcacctcgg gcttccagcg tccgatgtcg gacgtgtcgt
     4621 tccagagctt cgacatgggc aggtccgtcc gctcgacctc gtaggcggcc aggtagcggg
     4681 gctcgcccgc ggtctcggtc cggtagcggg ccacgttcac gcagccgggc agcttcagca
     4741 gcagcggcac gtgctcgacg tcgtagagct cgttgaagag cgcctcgcgg tgcggctcga
     4801 tgtccatcat gacccagaag acgtacgggc tccggtaggt gagccccggg ctgctcccca
     4861 cccactcgta ggccgcgagg tgccggttca tggtgtgggg ccggatgtcc ttgggccagc
     4921 gcccggcctc cccggccgct ttccactcgg ggctctgcag caccgccagg ctctcgagct
     4981 cgtacgcggc cacgtagcgc ggctcggtag gcgacgactg ccgatagcgg ctggcccgga
     5041 tgaccccggg caccgcctgc agcagcggca cgtgctcctt gtcgtagacc tcgttgaaca
     5101 gcgcctcgtg gtcgtgggcc acgtccatcc gcaccaggaa caggccgcgt ggggccatcg
     5161 tcgctctcct ctcccccctt gctccgagcg ggcttccctc gtatcttcgt cgggaccggc
     5221 tgccggcggc tgctgcccgg ctgaatgtac cgcgccggga gtggccgcgc acacaaacct
     5281 ctcaagggag acgaccccga tgccgccagt agacccgaac gaagtcctga tgaccggcga
     5341 gaactccttc atccgcctga gccccgacgg cggcaagacg ctctccgacc gcgcgagcca
     5401 ctggcgcgtc ctctggtgcc cggcgggcgg aggccacgtg ctctttctcc agtccacgct
     5461 cacggacgga cgggtgcgga tctacagcga caacatcgcg gtggcgcggt ggctccagac
     5521 gaccatcgag aagctgctct tccccgcctt cgccgacacg tcgctgccgg tggtcgcggc
     5581 gcgcttcgac cggcacggcg gcccctggtc caccgccacc gacgcccggg tcacgatgtc
     5641 ctggtacgac tgcatcgagc ccttcgtgct caacgcccca cccggcttca acgaccggcc
     5701 gatcggcgtc ttcagcacct tcttcccggc ccgcgcggcc cagctcgagg tgaacggcaa
     5761 ggtcgcctcc ggccaggtgt gggccgagat gcgcggagac cgccagggca gcagcgccac
     5821 gctcgcgtgg tccgagacct gggtgaagcc gcgcggatag cggagtccat cgtgagcgga
     5881 cggctcgacg gcaaggtcgc catcgtcacc ggaggtggaa gcggcatcgg ccgcgccagc
     5941 gcgctcacct tcctgcgcga gggcgcccgg gtggtcatcg gcgatctgaa cgacgcgagc
     6001 ctcgccgaga ccgtcgcgct cgcgaaggcc cagggcggcg ccgaccgcct cgccgcgctc
     6061 cgggccgacg tctcggcgga gcgcgacgtg gcggccctgg tggcgctggg ggtggagcgg
     6121 ttcggcggcc tcgactgtct cttcaacaac gcgggcgtcg gcggcgcctt cgggccgatc
     6181 ggcgagacca ccgtcgagga gtgggacttc accttcgcgg tgctggtgcg gggcgtgttc
     6241 ctcgggatca agcacggcgc cacgcgcatg aaggcgcagg gccgcggcgg cagcatcgtc
     6301 aacaccgcgt cgatcgcggg gatcggcggc ggggccgggc cccacgccta ctccgccgcg
     6361 aaggcggcgg tcgccaacct gagccggtcg gtctccgccg agctggccgc gcatcgcatc
     6421 cgggtgaacg cgatcgcacc cggcatgatc aagaccccgc tcgccacggg ccgccgggag
     6481 gaggcctggg atcgcctcgc gcgggagaag cagccgtggc ccgacctggg tctgcccgag
     6541 cacatcgccg acgccgccct cttcctggcc agcgacgact cgcgcttcat caccggccag
     6601 gtcctcgtgg tggacggcgg cctcaccgcg atgggcccgg atctcttcgg gcacgaggcg
     6661 ggcagccgca tgctccgcaa ggcggggctc aacacggggt cgaccgggc
//
LOCUS       DATMCV010000250         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_415153, whole genome shotgun sequence.
ACCESSION   DATMCV010000250 DATMCV010000000
VERSION     DATMCV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415153"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VNU03_05790"
     CDS             complement(<1..473)
                     /locus_tag="VNU03_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74003.1"
                     /translation="MRAPVAAMILALALGACASSSLNLPYKPEVQPSGATISAAYTIV
                     ADRLRVEIATEGRRLERAQVVRPDGGLLEAQTIEYTPPVGGGGSPVGIGFGIGSFGGM
                     GGGVSTGVGVSTGTTVGGGSSSSAVGPSTIASFPLAYAGPSPWRVRVKLQGIEPA"
     gene            509..1084
                     /locus_tag="VNU03_05795"
     CDS             509..1084
                     /locus_tag="VNU03_05795"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin family protein"
                     /protein_id="HWP74004.1"
                     /translation="MSVEDEEARVPPGADLERYLRELAEFVGLSEADAVVIRESAPAV
                     LKHEAALTSALYEHFLRFPATARFFLAPDGTPDRTRIERRKHSLARWLKETAEAAITH
                     DFAYYLLMIGISHSHREHGPGGKIPPQFMVAAMSLTQTALAGIFRDELGDPARALAAA
                     VAWNKLLLLHLNVLLIGYLMPPRPAPHGSSD"
     gene            1137..>2171
                     /locus_tag="VNU03_05800"
     CDS             1137..>2171
                     /locus_tag="VNU03_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWP74005.1"
                     /translation="MAKPTSIRELRDSGYVARSVKQELRDNLIARLRKGEGLFSGIVG
                     YEQTVVPQIENAVLSGQDIVFLGERGQAKTRMARLLVGLLDEEMPALAGCEISDEPFA
                     PICSACRQRVAEQGDKTPIAWIPRDRRYGEKLATPDITIADLIGEVDPIKVAEGRYLS
                     DELTIHYGLLPRTNRGIFALNELPDLAERIQVGLLNIMEERDVQIRGYKVRLPIDLYV
                     VASANPEDYTNRGRIITPLKDRFGSQIRTHYPRRVEDEIAIMESERTAYTDVGLETRS
                     PEFMKQLVAEVTHLARRSPEISQRSGVSVRVTICNYENLLSSALKRAIRLGETVAAPR
                     VSDLGAIAAST"
BASE COUNT          352 a          772 c          761 g          286 t
ORIGIN      
        1 tcggcgggct caatgccctg cagcttgacg cgcacccgcc aggggctcgg cccggcgtag
       61 gcgagcggga acgaggcgat ggtggacggg cccaccgcgg aggaggagct gcctccgccc
      121 accgtggtcc cggtcgagac gccgacgccg gtgctgacgc cgccgcccat gccgccgaag
      181 ctaccgatgc cgaacccgat cccgaccggg ctgccgccgc cgcccaccgg tggggtgtac
      241 tcgatggtct gggcctcgag cagcccgccg tcgggccgga cgacctgcgc ccgctccagc
      301 cggcggccct cggtggcgat ctcgacgcgc aggcggtcgg ccacgatggt gtaagcggct
      361 gagatggtgg ccccgctcgg ctggacctcg ggcttgtagg gcaggttgag cgaggaggac
      421 gcgcaggcgc cgagagccag cgccaggatc atcgccgcga ccggggcccg catgggcggt
      481 attatcgcac gcggacagga ggcagtgcat gagcgtggaa gacgaggagg cccgggttcc
      541 cccgggggcg gatctcgagc ggtacctgcg cgagctggcc gagttcgtgg ggctcagcga
      601 ggccgacgcg gtggtgatcc gggaaagcgc gccggcggtg ctcaagcacg aggccgcgct
      661 gaccagcgcg ctctacgagc acttcctccg cttcccggcc accgcgcggt tcttcctggc
      721 cccggacgga acgccggatc gcacccgcat cgagcgccgc aagcacagcc tcgcccgctg
      781 gctcaaggag accgccgagg ccgcgatcac ccacgacttc gcctactacc tgctgatgat
      841 cggcatctcg cacagccacc gggagcacgg cccgggcggc aagattccgc cgcagttcat
      901 ggtggccgcg atgagtctca cccagaccgc gctggccggg atcttccgcg acgagctggg
      961 agacccggcg cgggcgctcg cggcggcggt ggcgtggaac aagctgttgc tgctccatct
     1021 gaacgtgctg ctgatcggct acctgatgcc gccccggccc gcgccgcacg gctcgtccga
     1081 ttgacagcgg gcaccgtccg ccgcatagtg agcgcacgat agcgaaggga gccctcatgg
     1141 cgaagccgac ctcgatccgg gagctgcgcg acagcgggta cgtcgctaga tctgtcaagc
     1201 aggagttgcg cgacaacctc atcgcgcgcc tgcggaaggg cgagggactc ttctcgggca
     1261 tcgtgggcta cgagcagacc gtcgtccccc agatcgagaa cgcggtgctg agcggacagg
     1321 acatcgtgtt cctcggcgag cggggccagg ccaagacccg catggcccgc ctgctggtgg
     1381 gactgctcga cgaggagatg ccggccctcg cgggctgcga gatcagcgac gagccgttcg
     1441 cgccgatctg ctcggcgtgc cggcagcggg tggccgagca gggcgacaag acgccgatcg
     1501 cgtggatccc gcgcgaccgc cgctacggcg agaagctcgc gacccccgac atcaccatcg
     1561 ccgacctcat cggcgaggtg gatccgatca aggtggcgga gggccgctac ctctccgacg
     1621 agctgacgat ccactacggg ctgctgcccc gcacgaaccg cggcatcttc gccctgaacg
     1681 agctgccgga cctggccgag cgcatccagg tcggcctgct caacatcatg gaagagcgcg
     1741 acgtgcagat ccgcggctac aaggtgcggc tgcccatcga tctctacgtg gtggcgagcg
     1801 ccaacccgga ggactacacc aaccgcggcc gcatcatcac cccgctcaag gaccgcttcg
     1861 gctcccagat ccgcacccac tatccccggc gggtcgagga cgagatcgcg atcatggagt
     1921 ccgagcgcac cgcctacacc gacgtgggcc tcgagacgag gagcccggag ttcatgaagc
     1981 aactggtggc cgaggtcacc cacctcgcgc ggcggtcgcc cgagatcagc cagcgctccg
     2041 gggtctcggt gcgcgtcacc atctgcaact acgagaacct gctctcgagc gcgctcaagc
     2101 gggcgatccg gctcggggag acggtggccg ccccgcgggt cagcgacctc ggcgccatcg
     2161 ccgcctccac c
//
LOCUS       DATMCV010000251         2382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_147920, whole genome shotgun sequence.
ACCESSION   DATMCV010000251 DATMCV010000000
VERSION     DATMCV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147920"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VNU03_05805"
     CDS             <1..202
                     /locus_tag="VNU03_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWP74006.1"
                     /translation="EFAEAASGVVGLETAVPLLLDRLVRPGVVDLPTLVARLSTGPAR
                     LLNLPGGSLAPGAAGDVTILDL"
     gene            322..1446
                     /gene="carA"
                     /locus_tag="VNU03_05810"
     CDS             322..1446
                     /gene="carA"
                     /locus_tag="VNU03_05810"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWP74007.1"
                     /translation="MTRPAQLALADGRVFRGEALGASGEGHGEVVFNTSMTGYQEILT
                     DPSYRGQMICMTYPLIGNYGINLEDVESRRPWLSGFIVKEACPYPSSWRSRIPLDAYL
                     RENGIVGIQGIDTRALTRHLRDHGAQEGIISTEERDGARLAERARALPGLLGRDLVTE
                     VSVEAPHGWTEGPWDLARGYTRPATASLRVVAFDSGIKQNILRCLAGLGCDVQVVPAS
                     TGAAAVLERKPHGIFLSNGPGDPEAVPYLVETVRGLVGKVPIFGICLGNQILGLALGG
                     STYKLKFGHHGGNHPVKDLETGRVEITAQNHGFAVDPRSVEKLGLVETHVNLNDGTSE
                     GMRHRELPIFSVQYHPEASPGPHDAHYLFRRFADLMTKGG"
     gene            1450..1716
                     /locus_tag="VNU03_05815"
     CDS             1450..1716
                     /locus_tag="VNU03_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74008.1"
                     /translation="MPKYLIAYRKTEESGQKPEWAVFTTDSDLSLEAHAVKERVEKRM
                     SVLGEKLWGNGEAIWIGNGRLDDVLYRRDEAAPEMSIVYGSIDA"
     gene            1725..>2382
                     /gene="carB"
                     /locus_tag="VNU03_05820"
     CDS             1725..>2382
                     /gene="carB"
                     /locus_tag="VNU03_05820"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243131.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HWP74009.1"
                     /translation="MPKRTDLRKILLIGSGPIVIGQACEFDYSGTQACKALREEGFEV
                     VLVNSNPATIMTDPEMADRTYIEPLTPEYVAKVIERERPDAMLPTVGGQTGLNLAVAL
                     AEDGTLDRYGVELIGAKLPAIKTAEDRNLFGAAMERIGLAMPLGFYASSPEDALAGMR
                     QRRMQFPLIIRPSFTLGGTGGSVAYHPEELETAIKWGLQQSPLQQVLVEESVIGWKEF
                     E"
BASE COUNT          375 a          838 c          834 g          335 t
ORIGIN      
        1 cgagttcgcg gaggcggcca gcggcgtcgt cgggctcgag actgcggtgc cgctcctgct
       61 cgaccggctg gtgcgccccg gggtggtgga cctgcccacc ctggtcgccc ggctcagcac
      121 cggaccggcg cgcctgctga acctgcccgg cggcagcctc gcgcccggtg cggcgggcga
      181 cgtcaccatc ctcgatctgt agcgggcctt cacggtcgac ccggctgcgt tccactcgcg
      241 gagccggaac acgcccttcg gcggcttcac cggaaccggc gccccgtgga tgacgctggt
      301 cggcggggtc aaggtgacgc cgtgacgcgc ccggcccagc tcgcgctcgc cgacggccgc
      361 gtgttccgcg gggaggctct cggcgcctcc ggcgaaggcc acggcgaggt ggtcttcaac
      421 acctcgatga ccggctacca ggagatcctc accgatccgt cgtaccgcgg gcagatgatc
      481 tgcatgacct acccgctcat cggcaactac gggatcaacc tggaggacgt cgagtcgcgc
      541 cgcccctggc tcagcgggtt catcgtcaag gaagcctgtc cctacccctc gagctggcgc
      601 tcgcggatcc cgctcgacgc ctacctgcgc gagaacggga tcgtcggcat ccagggaatc
      661 gacacccgcg cgctcacccg tcacctgcgc gaccacggcg cgcaggaagg catcatctcg
      721 accgaggagc gggacggcgc gcgcctcgcc gagcgggcgc gggccttgcc cggattgctc
      781 gggcgcgacc tggtcaccga ggtgagcgtg gaggctcccc acggctggac cgagggcccc
      841 tgggacctgg cgcgcggcta cacgcgaccc gccaccgcga gcttgcgggt cgtcgccttc
      901 gattccggga tcaagcagaa catcctgcgc tgcctggccg ggctcggctg cgacgtgcag
      961 gtggtgccgg cgagcaccgg ggcggccgcg gtgctcgagc ggaagcccca cggcatcttc
     1021 ctctccaacg ggccgggcga tcccgaggcg gtgccctacc tggtggagac ggtgcggggg
     1081 ctcgtcggca aggtgccgat cttcggcatc tgcctcggga atcagatcct cgggctcgcc
     1141 ctcggcggca gcacctacaa gctcaagttc ggccatcacg gcggcaacca tccggtcaag
     1201 gatctcgaga ccggccgggt ggagatcacc gcccagaacc acggcttcgc ggtggatcca
     1261 cgatcggtgg agaagctcgg gctggtggag acgcacgtca acctcaacga cggcacctcg
     1321 gagggcatgc ggcaccgcga gctgccgatc ttctcggtgc agtaccaccc ggaggcctcg
     1381 ccgggcccgc acgacgcgca ctacctgttc cgtcgtttcg ccgacctcat gacgaaggga
     1441 gggtgagcca tgcccaagta cctcatcgcc taccgcaaga cggaggaatc gggccagaag
     1501 ccggagtggg cggtcttcac gaccgacagc gacctgagcc tcgaggcgca cgcggtcaag
     1561 gagcgcgtcg agaagcgcat gagcgtgctc ggcgagaagc tctggggcaa cggcgaggcg
     1621 atctggatcg gcaacgggcg cctcgacgac gtgctctacc ggcgtgacga ggccgcgccg
     1681 gagatgtcga tcgtctacgg gtcgatcgac gcctaggagc ctacgtgccg aagcggaccg
     1741 acctgcgcaa gatcctcctc atcggctccg ggccgatcgt catcggccag gcctgcgagt
     1801 tcgactactc gggcacccag gcctgcaagg ccctccggga ggaaggcttc gaggtcgtgc
     1861 tggtgaacag caacccggcc accatcatga ccgatcccga gatggccgac cgcacgtaca
     1921 tcgagccgct cactcccgag tacgtggcca aggtgatcga gcgcgagcgg cccgacgcga
     1981 tgctgccgac ggtgggcggg cagaccgggc tcaacctggc ggtggccctc gcggaggacg
     2041 gcacgctcga ccgctacggg gtcgagctga tcggggccaa gctgcccgcg atcaagaccg
     2101 cggaggaccg caacctcttc ggcgccgcga tggagcgcat cgggctcgcg atgcccctgg
     2161 gcttctacgc gtcgagcccg gaggacgccc tcgccggcat gcggcagcgg cgcatgcagt
     2221 tccccctgat catccggccc tccttcaccc tcggcggcac cggcggctcg gtcgcctatc
     2281 acccggagga gctggagacc gcgatcaagt gggggctcca gcagagcccg ctccagcagg
     2341 tgctggtgga ggagtcggtc atcgggtgga aggagttcga gc
//
LOCUS       DATMCV010000252         6016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_879054, whole genome shotgun sequence.
ACCESSION   DATMCV010000252 DATMCV010000000
VERSION     DATMCV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879054"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..137
                     /locus_tag="VNU03_05825"
     CDS             6..137
                     /locus_tag="VNU03_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWP74010.1"
                     /translation="MTLERIGNRLGLTRERVRQIEGAALNQLRGLLAARNVEPSDLQ"
     gene            159..377
                     /locus_tag="VNU03_05830"
     CDS             159..377
                     /locus_tag="VNU03_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWP74011.1"
                     /translation="MSPKNKKLGQVVRELRERQGLTQAQLAERAQLALSYITLLESGQ
                     EANLSPSAIGRLARALGITSRQLSEIGG"
     gene            380..763
                     /locus_tag="VNU03_05835"
     CDS             380..763
                     /locus_tag="VNU03_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74012.1"
                     /translation="MATDPLRKAHADGQRLRQAIDTEYRSARRDSTWGRTEPQMVERW
                     RLAVRAWTQAVEAAMGAQEALLGRFRTAPPTAEPAPAGESAAWAEIRNALAGKVATVG
                     KLIEEKSAGGSGPGGTPPPSPFRKR"
     gene            complement(786..1613)
                     /locus_tag="VNU03_05840"
     CDS             complement(786..1613)
                     /locus_tag="VNU03_05840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP74013.1"
                     /translation="MSPVDSGPHASLERMNRFQEVEARQIIRDLDLPAGSQGLDVGCG
                     VGLWALWLAEAVGPGGRVVGIEPEAEKVEAARALVGGKTEPGRLEFTPGDGTAIPLPD
                     RSVDWVWCGDVLHHVVETERALREFLRVVRPGGRVIVKESQVLSAMFLPGHPELERRI
                     QLAEIRRTLGEGAGRSFQERRQTTLASLHAVGLASATTRSYMLERRAPLAPADRDYIQ
                     HTVFSRNWGDRLRDLLTAEDWQARTRLCDEGSAANVLRSPAYYCLYPVTVFSTRAPA"
     gene            complement(1610..2455)
                     /locus_tag="VNU03_05845"
     CDS             complement(1610..2455)
                     /locus_tag="VNU03_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74014.1"
                     /translation="MKTMIRVAIGALLVGTVCLAAGSTPASATVIGIPGNAPTNLPAG
                     PADPNLAGYNLYPFMLSGFLPPSANPLPTGLFAESPLQFNLALQAGFGAAGKVDPLVV
                     PQTFNFFIIGDFWSVQAQLEYKPKSTFLFPSDIVTMTGDAVHRIAPHAGEFAPGPAVP
                     FNVVLNAGSVVTLPPDLDVPGAALQKAIQDAKLPAGARFGLDVRSVNHALGPDDDTVF
                     AILAGQIGSPGNPFVGNELEFWVGGVAGLHSPTPEPGVVLLWGTTALGLGVVARRRRR
                     GTVSA"
     gene            2618..3241
                     /locus_tag="VNU03_05850"
     CDS             2618..3241
                     /locus_tag="VNU03_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWP74015.1"
                     /translation="MIVLGAGALSLGLHAPTVAVAPQAAAPPLAGADLLAALRAGGNI
                     LYFRHADTDHSQVDRGVRLEDCTTQRNLTDRGRDHSRALGEAIRSLGIPIGAVLASPM
                     CRTMETATLAFGTALKSQAVREGGSLAPGTPGRFPALRELLSTKVAPGANTVIVGHAY
                     PYFTLVGGQYLNEGEADVVQPRGTDFEVLARLGLKQWRDLATLPVRQ"
     gene            3582..3743
                     /locus_tag="VNU03_05855"
     CDS             3582..3743
                     /locus_tag="VNU03_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74016.1"
                     /translation="MPRLLQVAPDGGGRYIAVDRDGQIWRGEAKRSKPDGTEVIAWTP
                     MPSEFPSGA"
     gene            3740..4024
                     /locus_tag="VNU03_05860"
     CDS             3740..4024
                     /locus_tag="VNU03_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74017.1"
                     /translation="MSRVTPLLALLGVLTLATSASAEGAWVLWLGTGTTYTPFGAYGG
                     ATGERECKEAVAQLMTEMRKNSTQLSEFLKSSSRYICLPDTVDPRGSKGK"
     gene            4077..4442
                     /locus_tag="VNU03_05865"
     CDS             4077..4442
                     /locus_tag="VNU03_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74018.1"
                     /translation="MPKRSKPVDPTFLLASRIARPVGGPLPHDLPVAAWARLFVVALL
                     RLERAKPHWASLTASEVENLLASWSVFEQDKSLSKAELDMRLEEALEQLEGQPPWAMK
                     ARRPAESPRGRRPKKGSRR"
     gene            complement(4460..5017)
                     /locus_tag="VNU03_05870"
     CDS             complement(4460..5017)
                     /locus_tag="VNU03_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWP74019.1"
                     /translation="MLAIWVKVKIKAAMRDRFLKAIEADALGSERDEPGCLRFNVFQD
                     EKDENVYYLFEVYKDQAALEAHCNTPHYAMWGGVADVLEGPLAATSTAGAIRRLGGTR
                     WQWLRRLVYVIGLWPTEPTRCRPVFPAASAYWGKMNDPRQGVPLTRVGSAGQEPLDRP
                     PFLPLLDPAPVSGRLSLGPDPVAGA"
     gene            5537..5743
                     /locus_tag="VNU03_05875"
     CDS             5537..5743
                     /locus_tag="VNU03_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74020.1"
                     /translation="MELLPKKAQEDLTKSGIRYHPEFGEYLLKVGEHMWDGRTDAGLA
                     RIKRRGLAALASEEAKKAAAGPAR"
     gene            5740..5871
                     /locus_tag="VNU03_05880"
     CDS             5740..5871
                     /locus_tag="VNU03_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74021.1"
                     /translation="MSEAREVVECGGCNRILLIPVAWGVVAYLCLACRGRLAAPRAA"
BASE COUNT          932 a         2088 c         2166 g          830 t
ORIGIN      
        1 agccgctgac cctcgagcgc atcggcaatc gcctgggcct cacccgtgag cgggtgcggc
       61 agatcgaggg cgcggccctc aaccagctgc ggggcctgct cgccgctcgc aacgtcgagc
      121 cttcggatct acagtgaggc gccggcagtg aggcgccggt gagccccaag aacaagaagc
      181 tcggccaggt cgtgcgcgag ctgcgcgagc ggcaggggct cacccaggcc cagctcgcgg
      241 agcgggccca actcgcgctc agctacatca cgctgctcga gtccggccag gaggcgaacc
      301 tctcgccctc cgccatcggg cgcctggccc gcgccctcgg catcaccagc cggcagctca
      361 gcgagatcgg cggctgaccg tggccaccga cccgctccgc aaggcgcacg cggacggcca
      421 gcgcctgcgg caggccatcg acaccgagta ccgctccgcc cgccgcgact ccacgtgggg
      481 ccgcaccgag cctcagatgg tcgagcgctg gcgcctggcg gtgcgcgcct ggacccaagc
      541 cgtcgaagca gccatggggg cccaggaggc gctgctgggt cgcttccgga cggcacctcc
      601 gaccgcggag cccgccccgg ccggcgagag cgcggcgtgg gcggagatcc ggaacgcgct
      661 ggcgggcaag gtggcgaccg tcggcaagct gatcgaggag aagagcgccg ggggctccgg
      721 cccaggcggc accccgccgc cgagcccgtt ccgcaagcgc tgagcgacgc gcggctgcgg
      781 cgccctcagg cgggcgcgcg cgtcgagaac accgtgaccg ggtagaggca gtagtaggcc
      841 gggctccgca gcacgttcgc cgccgagccc tcgtcgcaga gccgggtgcg cgcctgccag
      901 tcctccgcgg tcagcagatc gcgcagccga tcgccccagt tgcgcgagaa gacggtgtgc
      961 tggatgtagt cgcgatcggc cggggcgagc ggcgcccggc gctccagcat gtagctgcgc
     1021 gtggtcgcgc tcgcgagccc caccgcgtgc agcgaggcca gcgtggtctg ccgccgctcc
     1081 tggaaggagc gcccggcccc ttcgccgagc gtccgccgga tctcggcgag ctggatgcgc
     1141 cgctcgagct cgggatgccc gggcaggaac atcgcggaca ggacctgcga ctccttcacg
     1201 atgacgcgcc cgccgggccg cacgacgcgc aggaactcgc gcagggcgcg ctccgtctcc
     1261 accacgtggt gcagcacgtc gccgcaccac acccagtcca cgctccggtc gggcagggga
     1321 atcgcggtgc cgtcgcccgg cgtgaactcg agccgccccg gctccgtctt gccgcccacc
     1381 agcgcccgcg ccgcctccac cttctccgcc tcgggctcga tgccgaccac acgtccgccc
     1441 gggcccaccg cctcggccag ccagagcgcc cagagcccga ccccgcagcc cacgtcgagc
     1501 ccctgcgagc cggcgggcag atcgagatcg cggatgatct ggcgcgcctc cacctcctgg
     1561 aagcggttca tccgctcgag cgaagcgtgg ggaccggagt cgaccgggct catgccgaga
     1621 cggtgccccg ccggcgccga cgcgccacga ctccgagccc gagggcggtc gtgccccaga
     1681 gcaggaccac gcccggctcc ggcgtcggcg aatgcaggcc ggccacgccc ccgacccaga
     1741 actcgagctc gttgccgacg aacggattcc ccgggctccc gatctgcccg gccaggatcg
     1801 cgaagacggt atcgtcgtcc ggcccgagag catgattcac ggaccgcacg tcgagcccga
     1861 agcgggcccc cgccgggagc ttggcgtcct ggatcgcctt ctggagcgcg gcgcccggca
     1921 cgtcgagatc gggcggcaac gtcacgacac ttcccgcgtt cagcacgacg ttgaagggga
     1981 cggcggggcc cggcgcgaac tccccggcgt gcggcgcgat gcggtggacg gcgtcgcccg
     2041 tcatcgtcac gatatcgctc gggaacagga aggtcgactt gggcttgtac tcgagctgcg
     2101 cctgcacgga ccagaagtcg ccgatgatga agaagttgaa ggtctggggc acgacgagcg
     2161 ggtccacctt gccggccgct ccgaagccgg cctgcagggc caggttgaac tgcaacgggc
     2221 tctcggcgaa caggccggtc ggcagtgggt tcgccgacgg ggggagaaac ccgctgagca
     2281 tgaaggggta gaggttgtat ccggccagat tgggatccgc cggcccggcc gggagattcg
     2341 tgggcgcatt gccggggatg ccgatcacgg tggccgatgc cggcgtgctg ccggcggcca
     2401 ggcagacggt cccgacgagg agtgcaccga tcgcgacccg gatcatcgtc ttcatccctt
     2461 gttcctcctg gcacgcaggc tcggcccggc gccaggctag gtgaacctcg cagccctgtc
     2521 aatgcgtgct aggctccacg ctactggccg gcgccgggtg acggcgccaa ggaggaaggg
     2581 cgccatgcgg agtcgggcgc tgcggagtcg ggcgctaatg atcgtgttgg gtgccggagc
     2641 tctctcgctc ggcctccacg cgccgacggt cgccgtcgcg ccgcaggcag cggcgccacc
     2701 gctggcgggc gccgacctgc tcgcggccct gcgggcgggc ggcaacattc tctacttccg
     2761 tcacgccgac accgaccaca gccaggtcga ccgcggggta cggctcgagg actgcacgac
     2821 gcaacgcaac ctgaccgacc gcggtcgcga ccactcgcgc gcgctgggcg aagcgatccg
     2881 gtccctcggc atccccatcg gcgccgtgct tgcgagcccc atgtgccgga ccatggagac
     2941 ggcgacgctc gccttcggca ccgcgctgaa gtcgcaggcg gtgcgcgagg gtgggtccct
     3001 tgccccgggc accccggggc ggtttcccgc cctgcgcgag ctgctctcca ccaaggtggc
     3061 gcccggagcg aacaccgtca tcgtcgggca cgcctacccg tacttcaccc tggtcggcgg
     3121 acagtacctc aacgagggcg aggccgacgt ggtgcagccg cgggggaccg atttcgaggt
     3181 cctggcccgc ctgggcttga agcagtggcg cgacctggcc acgttgccgg tacggcaatg
     3241 agcgggagac ggagacgagc tggcgcggcg ctcgtgctgg gttgcctcgc catcgccacc
     3301 acggtccacg ccgagggcgg gtgggtgctg tggacccgct cgtgcagcgt caagagtgag
     3361 acgtgcgggg gcgactggca gcgacggcag acgtacgagg ccgagcggtg gtgtcgagcg
     3421 gcccggacca ccctcgtcga tcaggcgttg accccggaag cgcgagagac cgccatgagg
     3481 aaagggtcgg tcgtggagta tcagtgcctc cccgaggcca ccgacccgcg tcggccgaag
     3541 gcaccgtaac cgaagagtga cgagagagga gtactcgagc gatgcccaga ctgctacagg
     3601 tggcccccga cggtggcggc cgttacatcg cggtggatcg cgacggccag atctggcgag
     3661 gcgaggcgaa gcggagcaag cccgacggca ccgaggtcat cgcgtggacg ccgatgccgt
     3721 cggagttccc gagcggcgct tgagccgcgt caccccgctg ctcgcgctgc tcggggtgct
     3781 caccctcgcg acgtcggcgt ccgccgaggg cgcgtgggtg ctctggctcg ggaccggcac
     3841 cacgtacacg ccgttcggcg cctacggggg agcgacgggg gagcgggagt gcaaggaggc
     3901 ggtcgcccag ctcatgaccg agatgcggaa gaacagcacc cagctgagcg agttcctcaa
     3961 atcgagcagt cgctacatct gcctgcccga caccgtggac ccccgcggat cgaaggggaa
     4021 gtgaccctgc gcccgggaga tgtctgcttt cttcagggta cgtgggggga acattcatgc
     4081 cgaagcggtc caagcccgtt gatccgacgt tcctcctggc gagtcgtatt gcgcggccgg
     4141 tcggaggtcc gcttccgcac gaccttccgg tagcggcctg ggctcgcctc tttgtcgtag
     4201 ccctcttgcg cctcgaacgg gccaagccgc actgggcatc cctcacggct tcagaggtcg
     4261 agaatctgct ggcctcgtgg tcggtcttcg agcaggacaa atcgctgtcg aaggcggaac
     4321 tcgacatgag actcgaggag gctttggagc agcttgaggg ccagccgcca tgggcgatga
     4381 aagcgcgccg gcctgcggag tcgccgcgtg gtcgccggcc caagaaaggc tcacgccggt
     4441 agaggggcgg ggggttaact catgcgccag ctactggatc tggcccgaga gaaagccgcc
     4501 ctgaaacggg agctggatcg agtaggggaa gaaacggcgg gcggtccagc ggttcctggc
     4561 cggcactccc gacacgcgta aggggcacac cttgccgagg atcattcatc ttgccccagt
     4621 aggcggaggc ggccgggaag accggcctgc accgggtggg ctccgtgggc cagagtccga
     4681 tcacgtagac gagccggcgc agccactgcc accgggtccc gcccaggcgc cgaatcgcgc
     4741 cggcggtgga ggtggcggcc agcggccctt ccaggacgtc ggccaccccg ccccacatcg
     4801 catagtgcgg cgtgttgcaa tgcgcctcca gcgcggcctg gtccttgtag acctcgaaga
     4861 ggtagtacac gttctcgtcc ttctcgtcct ggaacacgtt gaagcgcagg cagcccggct
     4921 cgtcccgctc cgaaccgagc gcgtcggcct cgatggcctt caggaacctg tcccgcatcg
     4981 ccgccttgat cttcaccttc acccagatcg cgagcatcgt ggccctcctt ggtgcttcgt
     5041 tagcgcaccc cgagggcgtc ttacagcttc gacatgccag attccgcggg gtctaggcgc
     5101 ccggaacccc gtcggccggg ccatgggcgc tacggagtgt gcgaggaccg ccccaagcgt
     5161 gtcaattcga ccttcgtcga tctattgaga ctttggtcga agggacatga tgcgctgcaa
     5221 gtcccccaac atcaggagcc ccccgacgac gttgaagtat gcacggaacc cgcccaagca
     5281 agccggggcg cggaagcggc tgacagtggg accacgcatg atcgcggcca tctacgcaca
     5341 cgtcgcctcg agcggcgggg agggctggcg gagtacccag gatgctctcg tcggtcagct
     5401 caccatcaca acgtcgtgcc cggctcgggg ccctcgcgga acgggccccg gtcctgagtg
     5461 gcgaggaggc cgagttggcc ctcgagcagc ggtgggggag tcggtacgag cagccgatcg
     5521 cgcgggccac ggaggtgtgg aactgctccc caagaaggcc caggaggatc tcacgaagag
     5581 cggcatccgc tatcacccgg agttcgggga gtacctgctg aaggtgggcg agcacatgtg
     5641 ggacgggcgt acggatgctg gcttggcgcg gatcaagcgg cgcggtctcg cggcgctcgc
     5701 gtcggaggag gcgaagaagg ccgcggccgg gcctgcgcga tgagcgaagc gcgcgaagtg
     5761 gtcgaatgcg gcggctgtaa ccgcatcctg ttgatcccgg tggcctgggg cgtggtggcg
     5821 tatctctgcc tcgcgtgtcg cggccgcctg gccgcgcccc gcgccgcatg agtcgactcg
     5881 gcgtggtggt cacccggcgg ttcgtggagc gggttcaccg cgccgaccgc gtggtcgaag
     5941 cccgccgcgc cgtggtgctg ccgttcccgc ccacgccggg catgctgctg acgttcggag
     6001 acggctcggg tgagtg
//
LOCUS       DATMCV010000253         2582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_614314, whole genome shotgun sequence.
ACCESSION   DATMCV010000253 DATMCV010000000
VERSION     DATMCV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614314"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1870)
                     /locus_tag="VNU03_05885"
     CDS             complement(140..1870)
                     /locus_tag="VNU03_05885"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HWP74022.1"
                     /translation="MMKRATVESTADAYLELLAARGVEYFFANAGTDFAPLIEAYARR
                     SAQGQASPRPITVPHEVPAVAMAHGYTMVTGRAQVVMVHVIVGAGNAAGGVINAARSN
                     VPMLFSAGRNPITEAGDAGSRDRPIHWAQEAFDQAGMLREFVKWDYELKRFDQLETVV
                     DRALTLAQSEPRGPVYLTLPREVLAERHEAIEYADPARAVVPAATLPDPRAIDEAAAM
                     LAGARNPIIITKALGRDPAAVPVLVRFAETLGAPVFDQFHTYVNFPQDHPLHAGFEPL
                     DGRFEPAAHLSEADVILVLESDVPWFPQLNRPKPEARVIHVAVDPLFSRYPVRGFPAD
                     LALAGAPRLTLAALADALAGRVDPVAVAERRQRWTQAGARRREAALAKGQAARAEAPL
                     NMLWVSRAIGDVLDDRTIVVNEYDLDPTQCTFTRPGTYFAAPPSGGLGWGLGAALGAK
                     LASPERDVICCVGDGAYIFGSPTASHFVSRAYNLPVLFVVFNNRTWNAVKRAVQTYAP
                     DGWGVRTDAMPLTALEPSPDYEMLCQASGGHGEKVEDPAALPDALRRGLRIVREEKRQ
                     VVLNVICKKP"
     gene            2004..2573
                     /locus_tag="VNU03_05890"
     CDS             2004..2573
                     /locus_tag="VNU03_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74023.1"
                     /translation="MKAIGGLGIAIGMGAMALLASGCANIEHYAVEHDPVRVHWQQRA
                     DQVTRHFGVLPVHVMVVDATLDGAYTWSTATIELGRDNSDLGMRWLLAHELGHHLDRS
                     EGGSQLEREMTANRTAVQILQVWGASTDDAVTLVEAMLFTSQLRRAVVSGHNWCAELT
                     DIHAHFSDSRYDPAVVVSKCPGQEPLSSG"
BASE COUNT          386 a          866 c          942 g          388 t
ORIGIN      
        1 gccgccgcac ccaggtcggc ctccgaatag gccgcgcgcc ccggttggtc gagcgggcgg
       61 ccgatcgcca cctcgtggcc ggccgcgcgg agcgggtcga gctcgccggg cggaccgctg
      121 ggccccgagg cgaggatctt cacggcttct tgcagatcac gttcaggacg acctgccgct
      181 tctcttccct gacgatgcgg agcccgcgtc gcagggcgtc cggcagcgcg gccgggtcct
      241 ccaccttctc gccgtgcccg cccgaggcct ggcagagcat ctcgtagtcg ggcgagggct
      301 cgagcgcggt gagcggcatc gcgtcggtgc ggacccccca gccgtcgggg gcgtaggtct
      361 gcaccgcgcg cttcaccgcg ttccacgtgc ggttgttgaa gaccacgaac agcaccggta
      421 ggttgtaggc ccgcgagacg aagtgcgacg cggtcgggga gccgaagatg taggccccgt
      481 cgccgacgca gcagatcacg tcgcgctccg gcgaggccag cttggccccc agcgcggcgc
      541 ccagccccca gccgagcccg cccgagggcg gcgccgcgaa gtaggtgccg gggcgggtga
      601 acgtgcactg ggtgggatcc agatcgtact cgttcaccac gatggtgcgg tcgtcgagca
      661 cgtcgccgat ggcgcgcgag acccagagca tgttcagcgg tgcctcggcg cgcgcggcct
      721 gccccttcgc cagcgccgcc tcacgccggc gggccccggc ctgggtccag cgctggcggc
      781 gctcggcgac cgcgacgggg tccacccgcc cggcgagcgc atccgccagc gccgccagcg
      841 tcagccgcgg tgcgcccgcg agcgccaggt cggcggggaa accgcgcacc gggtagcgtg
      901 agaagagagg gtccaccgcc acgtggatca cccgcgcctc gggcttcggc cggttcagct
      961 gcgggaacca cggcacgtcc gactcgagca cgaggatgac gtcggcctcg ctcagatggg
     1021 ccgcgggctc gaagcggccg tcgagcggct cgaagccggc gtgaagcggg tgatcctgcg
     1081 ggaagttcac gtaggtgtgg aactggtcga agaccggggc cccgagcgtc tcggcgaagc
     1141 gcaccagtac gggcaccgcg gccggatcac ggccgagcgc cttggtgatg atgatcggat
     1201 tgcgcgcgcc ggccagcatc gcggccgcct cgtcgatcgc gcgcggatcc ggcagcgtgg
     1261 ccgccggcac caccgcccgc gcggggtcgg cgtactcgat ggcctcgtgc cgctcggcca
     1321 gcacctcgcg cggcagcgtg agatagaccg ggccgcgtgg ctcggactgg gccagggtca
     1381 gcgcccggtc caccaccgtc tcgagctggt cgaagcgctt cagctcgtag tcccacttga
     1441 cgaactcgcg cagcatgccc gcctggtcga acgcctcctg cgcccagtgg atgggccggt
     1501 cgcggctacc cgcgtccccc gcctcggtga tcgggttgcg cccggccgag aagagcatgg
     1561 gcacgttgga gcgcgccgcg ttgatcaccc cgcccgccgc gttgcccgcg cccacgatga
     1621 cgtggaccat caccacctgg gcgcggccgg tgaccatggt gtagccgtgc gccatcgcca
     1681 ccgccggaac ctcgtgcggc accgtgatgg ggcgcggact cgcctggcct tgcgcgctcc
     1741 ggcgcgcgta ggcctcgatg agcggggcaa agtcggtgcc cgcattcgcg aagaagtact
     1801 ccactccgcg cgcggccagc agctcgagat aggcgtcggc ggtggactcg acggtcgctc
     1861 gtttcatcat ggtgctgggg agggtagcgc cgggcggtgg ggtggtcaag gaccatccgt
     1921 actaggcaat cgcgccgcgg gtcgaagcga ttccggaact cgcgagactc ctgactgctc
     1981 taaagaagtg gagggggtat gccttgaaag cgatcggcgg cctgggtatt gcgatcggca
     2041 tgggagcaat ggccttgctc gcatccggct gcgccaatat tgagcactat gccgtcgagc
     2101 atgatccggt acgcgtgcac tggcagcagc gcgctgacca ggtgacgaga cactttggcg
     2161 tgctcccggt tcacgtcatg gtggtggatg ccacgctgga cggcgcgtac acgtggtcaa
     2221 cggcaaccat tgagttgggt cgggacaact cggatctcgg gatgcgctgg ctgttggccc
     2281 acgagctagg gcatcatctc gatcgatctg agggagggag tcagctcgag cgagagatga
     2341 cggccaatcg aacggcggtg cagatcctcc aggtatgggg cgcttcgact gacgacgccg
     2401 tgacgttggt tgaggcgatg ttgttcacga gccagctgcg ccgagcggta gtatccggcc
     2461 ataactggtg cgcggagctg acggacatcc acgcgcattt ctcagacagc cgctatgacc
     2521 ccgctgtggt ggtctcgaag tgtccgggac aggagccgct gagctccggt taagagagtt
     2581 cg
//
LOCUS       DATMCV010000254         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_415637, whole genome shotgun sequence.
ACCESSION   DATMCV010000254 DATMCV010000000
VERSION     DATMCV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415637"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VNU03_05895"
     CDS             <1..1939
                     /locus_tag="VNU03_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP74024.1"
                     /translation="RVVGEVAGPTGGVTVPDTVHGVLMARIDQLPEAPKRLLQTASVL
                     GRAFSVRVLEVIFDEAGPLAPLLLELARLEFLYEETGAEEPVYVFKHALTQDVAQTTL
                     VVPRRRALHRRAAEALETLYPARMHELAPVLAHHYSEAEAWGPAVAHATRAAEAARGA
                     YANREALARYDQAITAAERAGLSPALRIPLLEARAEVAAVLGEFERARGDLEAALNLV
                     ETLGEPTGRGRLLWTLGALWGGHKDYQKGIELTRRAVAVLEAAGDRRVLAGARAQLGM
                     MELNLGRMAECRSELERALVLFRELGDEAGQARTLEMLAMNAWMVGAVSDTERYVDAA
                     LPRLRRLGDRGTELSALLSLAAVRSAHGGWEACEPAFRQALELAQATGARGAEAYVRA
                     AAAEFAIPLGLYGLAAEQAGIALDIAREIGHREWTALALAGRGRVLRACGDVAGARCV
                     HEEMLETARELGTSLWTADALGNLGEDLLQAGDDEAAARYLAEAIAKAGGGIKHALRP
                     LIAQAELGLRAGRAEDGLAAARRVAALAPEMRVFVAEARCIEGEALAMLGRSAEALAV
                     LGDAKAEAVAIGAAPPRWRSALALRRVLDRCGRRDEARREAAEARAVLERVSAQLPDP
                     QLRRLFGRSDAWREANTDSPS"
     gene            1973..2077
                     /locus_tag="VNU03_05900"
                     /pseudo
     CDS             1973..2077
                     /locus_tag="VNU03_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2077..>2495
                     /locus_tag="VNU03_05905"
     CDS             2077..>2495
                     /locus_tag="VNU03_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74025.1"
                     /translation="MDRRTFFGTLAGGLLAAPLAAGAQQARAVPRLGMLLTGSPSDMR
                     QSRELDALTKKLGELGWVEGRTLGVETRWAKNTDSLPGLAADLVRLGVDIILTPGPEA
                     TNAVRTATSAIPIVMIASTDPRAVGAAGLARPGGNVTG"
BASE COUNT          333 a          863 c          969 g          330 t
ORIGIN      
        1 ccgggtcgtc ggggaggtgg cgggaccgac cggcggcgtc acggtccccg acaccgtcca
       61 cggggtcctg atggcccgga tcgaccagct gccggaggct ccgaagcggc tgctccagac
      121 cgcctcggtg ctggggcgcg cattctcggt gcgagtgctc gaggtgattt tcgacgaggc
      181 gggcccgctc gcgccgcttc tcctcgagct cgcgcgcctc gagttcctgt acgaggagac
      241 tggcgcggag gagccagtct acgtgttcaa gcacgcgctg acccaggacg tggcccagac
      301 caccctggtc gttccgcggc gtcgcgccct gcaccgccga gccgccgagg cgctcgagac
      361 actgtatccg gctcggatgc acgagctggc gccagtgctg gcccatcact actcggaagc
      421 ggaggcatgg gggcccgcag tggcgcacgc cacgcgtgcg gccgaagcgg cgcgcggcgc
      481 ctacgcgaac cgcgaggcgc tggcgcgtta cgaccaggcg atcaccgcgg cggagcgggc
      541 gggcctgtcc ccggctctgc gcatcccgct gcttgaggcg cgcgccgagg tggccgcggt
      601 cctcggcgag ttcgagcgcg cgcgcgggga ccttgaggct gcgctgaacc tggtcgagac
      661 gctcggcgag ccgacggggc gtgggcggct cttgtggacg ctcggcgctc tctggggcgg
      721 ccacaaggac tatcagaagg ggatcgagct gacccgccgc gcggtggctg tgctcgaggc
      781 cgcaggcgat cggcgcgtgt tggccggggc gcgggcgcag ctcgggatga tggagctcaa
      841 cctcggccgg atggcggagt gtcggagcga gctcgagcga gccctcgtgc tcttccgtga
      901 gctcggcgac gaggcgggcc aggcgcgaac gctcgagatg ctggccatga acgcgtggat
      961 ggtcggggcg gtgagcgaca ccgagcgcta cgtcgacgcc gcgctgccgc ggctgcgccg
     1021 gctcggggac cggggcaccg agctgtcggc gctgctgagc ctggccgcgg tgcgcagcgc
     1081 ccacggggga tgggaggcgt gcgagcccgc gttccggcag gcgctcgagc tggcccaggc
     1141 gaccggagcc cgtggggccg aggcctacgt gcgggccgcg gccgccgagt tcgcgatccc
     1201 gctcggtctc tacggactgg ccgccgagca ggccggcatc gcgctcgaca tcgcccgcga
     1261 gatcgggcac cgcgaatgga ccgcgctggc gctggcgggg cggggccgcg tcctccgggc
     1321 gtgcggagac gtcgcgggcg cgcggtgtgt gcacgaggag atgctcgaga cggcgcgcga
     1381 gctcgggacg tcgctctgga cggccgacgc gctcggcaat ctcggggagg acctcctgca
     1441 ggccggcgac gatgaagcgg ccgcgcggta cctggccgag gcgatcgcca aggcgggcgg
     1501 cggcatcaag cacgcgctgc gccctctgat cgcccaggcg gagctcggac tgcgcgccgg
     1561 gcgcgcggaa gacggcctcg cggcggcccg gcgcgtggcc gcactcgccc cggagatgcg
     1621 cgtgttcgtg gcggaggccc ggtgcatcga gggggaagcg ctcgcgatgc tcggccggtc
     1681 cgcggaagcg ctcgcggtcc tgggcgatgc caaggcggag gccgtggcca tcggcgcggc
     1741 gcccccccgc tggcgcagcg ccctggccct ccgccgtgtc ctcgaccgct gcgggcggcg
     1801 cgacgaggcc cggcgggaag cggcggaggc acgtgcggta ctcgagcgcg tctccgctca
     1861 gctgcccgat ccgcagctgc ggcgactctt cgggcgctca gatgcatggc gtgaagcgaa
     1921 cacagacagc ccctcatgag gaagatctct ctggtcgtgc agccagctgc tgcaaccgac
     1981 caggttcgaa atggtcatca acctcaagac cgccaaagcc ctcggcctga cgatcccgcc
     2041 gtcgctcctg cagcgggcgg atcaggtgat cgagtaatgg atcggcgcac cttcttcggc
     2101 accttggcag gcggcctcct cgccgcgccg ctcgccgccg gagcgcagca ggcgcgtgcg
     2161 gtcccgcggt tggggatgtt gctcacgggg tccccatcgg acatgcgcca gtcgcgcgaa
     2221 ctggacgcct tgacgaagaa gctgggcgag cttggctggg tcgagggacg gaccctcggt
     2281 gtcgagaccc ggtgggcgaa aaacaccgac agcctgcccg gcctcgcggc cgacctggtc
     2341 cgactgggcg tcgacatcat cctgacgcca ggccccgaag cgacgaacgc ggtcaggacg
     2401 gcgacgtccg cgatccccat cgtcatgatc gcttcgaccg accctcgggc cgttggcgcc
     2461 gccggcctgg cccgcccggg cggaaatgtg acggg
//
LOCUS       DATMCV010000255         7269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_676610, whole genome shotgun sequence.
ACCESSION   DATMCV010000255 DATMCV010000000
VERSION     DATMCV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676610"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VNU03_05910"
     CDS             complement(<1..356)
                     /locus_tag="VNU03_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74026.1"
                     /translation="MPRRPLRSVALGLAIVVAAVCLAGAPAEAQGAKPKRGGVLNTVL
                     IEDPPGLIIHESATVSNTWPMSPCYSNLVFFDPLKGQESVDTVIPELAERWSWQDNYR
                     NLVFFLRKNVKWHDGKP"
     gene            complement(382..1161)
                     /locus_tag="VNU03_05915"
     CDS             complement(382..1161)
                     /locus_tag="VNU03_05915"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP74027.1"
                     /translation="MELQGQTAIVTGAGRNIGRAIALELAGMGADVMVAEVDRATGER
                     TAEEVRKRGRRAMALQTDVTRLADLQAMADRAVAEWGRVDILVNNAGIHKSAHTADVT
                     EADWDRLLAVNAKGVFFASQAVLRHMVKARRGNIISLASMAGKMGLKTSLVYGITKAA
                     VISMTRSLALAHAADGIRANCVCPGIVETDMIFQVDREAGEGLMGLKPGEYLKQRVEQ
                     IPLGRIEKAEDVANVVGFLASSKSSYMTGQAINVTGGMVMY"
     gene            complement(1178..1594)
                     /locus_tag="VNU03_05920"
     CDS             complement(1178..1594)
                     /locus_tag="VNU03_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP74028.1"
                     /translation="MPYRINHIHLKASDPRKTADWYVKAFAFKIVSDETRVFGDRFVR
                     CQSEDGTPVNISGARTNEKLGAGDARAHHGLEHFGFDSENLEADIKRLEGLGAKLLEG
                     PIEIPGGPRICFVQAPDDVRLELIQRSKASIAGGCC"
     gene            1707..2594
                     /locus_tag="VNU03_05925"
     CDS             1707..2594
                     /locus_tag="VNU03_05925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP74029.1"
                     /translation="MDIGCHLPNQGPLANGESLVRFARAAEERAVASLWVSDHVIFPR
                     SATGSYPGGRFPHPPDKPYLEPVTVLAAAAVCTSRARLGASVFILGHRHPVVMAKMLT
                     SIDALSNGRLICGVGVGWWKEELEILGVPFHARGRHGDEILRAFKALWTEENPRLDGE
                     FFRFRELGFAPKPVQKPHPPIWVGGDSPGAFRRVVTLGDGWHATSKDPDQLREGLGRL
                     RAVADQHRRDMKTIDISVRYSLSDGMLGKGAQMVIDQLGEYKRIGLCHVLIDFRRDDL
                     AKMLELLDLVTTTIRPAVA"
     gene            complement(2598..4016)
                     /locus_tag="VNU03_05930"
     CDS             complement(2598..4016)
                     /locus_tag="VNU03_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP74030.1"
                     /translation="MTAPSPGGSADETTILIVDDEPRVLDALEAILAAEFRVLRANHG
                     EEALERLAAEADVAVIVTDHRMPGMTGVELLRRSQERTPDAVRIVLTAYTDVDSLMEA
                     INTGRIYHFVPKPWDPNELLVVVRRAAERWRLARENARLRDELELAYNALRREATAGR
                     EKPASFDKLIGAETGLRGAVELARKVLDGDTSVLLLGETGTGKELFAHLIHTNGSRRT
                     RPFVAQSCGALPDTLLESELFGHARGAFTGAVGERKGLFEEADGGTIFLDEVGETSPA
                     MQLRLLRVLQEGEVRRVGGSGTRRVDVRLIAATNADLEADVAAGRFRRDLFYRLSVFP
                     VRLPPLRERAEDIPTLAEHFLRQACRRGRRAVPAVGPEALSLLRGYPWPGNVRELENE
                     IERAVALADDGRPLGPAHLSERIAAGAGAAPSVRTLSEAVEALKRRMIEDALRECGSK
                     TRAAEQLGLTRQSLQQMLRRRQ"
     gene            complement(4013..5176)
                     /locus_tag="VNU03_05935"
     CDS             complement(4013..5176)
                     /locus_tag="VNU03_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP74031.1"
                     /translation="MTPATVLLVDDEPRVLDALEALLAMEHRILRAERPEIALRHLRE
                     EDVAVVVSDQRMAGLTGTELLTRSRDVAPDTVRILLTAFTDTEALMDSINAAGVYHFL
                     LKPWDPNELRHVVGRAVEHHRLCRERETLLGNLAAKNAELEQALTHVRDAQDDLIREA
                     SVRSQLQRYVSPRLVDLAVANPGILELPGDWREATVLFADIRGFTRLIESTTAPVVIQ
                     LLNEYFTEMIDVIFRHQGTVEQLVGDEIVALFGVPEPGADVALRAVRAAADMVEAVRR
                     LAARWTADGRPTFDIGIGISSGRVLAGTIGSDRRRELIVVGRAMIAASRMQRMTRLFD
                     AHIIVGEETFHQVNHLVDARELGRPHLKGIRQRQTLYEICRLREPAVAVPAST"
     gene            complement(5173..>7269)
                     /locus_tag="VNU03_05940"
     CDS             complement(5173..>7269)
                     /locus_tag="VNU03_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP74032.1"
                     /translation="LMREPDEDRWARRYGESFMDWKPREERSYLGTYSMGIGNPRAAY
                     QRVSTIYEQQSRGLYRLDTLEVGRRHARYRWTPMAGQTIPVWSCTWRKVQFERFPTNW
                     GLPRAHILETTCAARGGDACRWDVRWKNPSRGPRFWAPSLIGGAAAALLALVAASPGG
                     SWPLLAASPLPLLAGVALGYALREGQQRRHTQRLLDLQSEEIIYSNRELEKKFVELET
                     RIEQLSLLTDLGAAVSGTLDPEKIYDQALSRLVHRMPYERAYLLLVDRERRVLRGHRS
                     AGADPAAPPFEGVELPLEATSSASARAAITGQPVLVSDVETATEPVHHALSRAHRVRS
                     LVAAPLRVREQVLGVLSVTSTEAGRFDRGDVDLLTAVASHVALAIDRAASFQTIEDLS
                     RSLEDKVRVRTEQLRAANEELRTAYRDLQTTQLQLIQREKMASVGQLVAGVAHELNNP
                     IGFISSNVATLADFVQRLRAMLATYQEAALPEADRARVDARRRELQVDYALRYLDSMI
                     SGIREGADRTRKIVGDLRVFTRTPDDVWQAVDLHEELDSSLTLLNHLLKDRVTVIRKY
                     GSLGPVECVRSQIDQVFLNVLANAAQAIPGPGTITIETRRVGPMAEIAIADTGLGIPP
                     DVLRRVFDPFFTTKPVGEGTGLGLSISYEIVTKHGGEIRAESPVDGGAVFTLRLPVAR
                     AGKPAETTPAAAPRVP"
BASE COUNT          965 a         2563 c         2665 g         1076 t
ORIGIN      
        1 ggcttgccgt cgtgccactt cacgttcttg cggaggaaga acaccaggtt ccggtagttg
       61 tcctgccacg accagcgctc ggccagctcc gggatcacgg tgtccacgct ctcctggccc
      121 ttgagcgggt cgaagaagac caggttcgag tagcacggcg acatcggcca cgtgttcgag
      181 acggtggccg actcgtggat gatgaggccg ggcggatcct cgatcagcac ggtgttgagg
      241 acgccgccgc gcttgggctt ggcgccctgc gcctcggccg gcgccccggc gaggcacacc
      301 gcggccacga caatggcgag cccgagggcg acggagcgga gcggacgtct cggcatgaag
      361 gtctccatgg gacgggtggg ctcagtacat caccatgcca ccggtgacgt tgatggcctg
      421 gccggtcatg taggacgact tgctcgaggc gaggaagccc accacgttgg ccacgtcctc
      481 ggccttctcg atccgcccga gcgggatctg ctccacgcgc tgcttgaggt actcgccggg
      541 cttgaggccc atgagccctt cgcccgcctc gcggtccacc tggaagatca tgtcggtctc
      601 cacgatgccg gggcagacgc agttggcgcg gatgccgtcg gcggcgtggg ccaaggccag
      661 gctccgggtc atgctgatca ccgcggcctt ggtgatgccg tagaccagcg aggtcttgag
      721 gcccatcttg cccgccatcg aggccaggct gatgatgttg ccccgcctgg ccttcaccat
      781 gtggcggagc accgcctggc tcgcgaagaa cacgcccttg gcgttgaccg cgagcagccg
      841 gtcccagtct gcctcggtca cgtccgcggt gtgggcggac ttgtggatgc ccgcgttgtt
      901 gacgaggatg tcgacgcgcc cccactcggc caccgcgcgg tcggccatcg cctgcaggtc
      961 ggccaggcgg gtgacgtcgg tctggagcgc catcgcccgg cgcccgcgct tgcgcacctc
     1021 ctcggcggtg cgttcgccgg tggcccggtc cacctccgcc accatcacgt cggcgcccat
     1081 gccggccagc tccagcgcga tggcgcggcc gatgttgcgc ccggcgccag tgacgatcgc
     1141 ggtctgccct tgcagctcca tctagatcgc ctccatctca gcagcagccg cccgcgatgg
     1201 acgccttgct tcgctggatc agctcgaggc ggacgtcgtc cggggcctgg acgaagcaga
     1261 tgcgcgggcc gcccgggatc tcgatcggcc cttccaggag cttcgcgccc agcccctcga
     1321 gccgcttgat gtcggcctcg aggttctcgg agtcgaagcc gaagtgctcg aggccgtgat
     1381 gggcccgggc gtcccccgcg ccgagcttct cgttggtgcg cgcgcccgag atgttcaccg
     1441 gggtgccgtc ctcgctctgg cagcgcacga agcggtcccc gaacacgcgc gtctcgtccg
     1501 agacgatctt gaacgcgaag gccttcacgt accagtcggc ggtcttgcgg gggtcggagg
     1561 ccttcaggtg gatgtggttg atccggtagg gcatggcggt gtcctttcgg ctgaggtatc
     1621 gtcggggccg cgcccgcggc gcggctagag aatagtagga cccgaccgcc ggttcaagga
     1681 ccggcgcatc ccggaggcca tcgcgcatgg acatcggctg ccacctcccc aaccagggcc
     1741 ccctcgccaa cggcgagtcg ctcgtgcgct tcgcgcgcgc ggccgaggag cgcgcggtcg
     1801 cctcgctctg ggtgagcgat cacgtgatct tcccgcgctc cgcgaccggc agctatccgg
     1861 gcgggcgctt cccgcatccg cccgacaagc cgtacctcga gccggtgacg gtgctggccg
     1921 ccgccgcggt ctgcacgagc cgggcccggc tcggcgcctc cgtgttcatc ctgggccacc
     1981 ggcatccggt ggtcatggcc aagatgctca ccagcatcga cgcgctctcc aacggccggc
     2041 tcatctgcgg cgtcggggtc ggctggtgga aggaggagct cgagatcctg ggcgtgccct
     2101 tccacgcccg cggccgccac ggcgacgaga tcctgcgcgc cttcaaggcc ctctggaccg
     2161 aggagaaccc gcgcctcgac ggcgagttct tccgcttccg cgagctgggc ttcgcgccca
     2221 agccggtgca gaagccgcat ccgccgatct gggtgggcgg cgacagcccc ggcgcgttcc
     2281 ggcgggtggt gacgctcggc gacggctggc acgcgacctc gaaggacccc gatcagctcc
     2341 gcgaggggct cggccgcctg cgcgcggttg cggaccagca ccggcgcgac atgaagacga
     2401 tcgacatctc ggtgcgctac agcctcagcg acgggatgct gggcaagggc gcccagatgg
     2461 tgatcgacca gctcggggag tacaagcgca tcggcctgtg ccacgtcctg atcgacttcc
     2521 gccgcgacga cctcgccaag atgctcgagc tgctcgacct ggtcaccacc accatccgcc
     2581 ccgcggtcgc gtagctctta ttgccggcgg cggagcatct gctggagact ctggcgggtg
     2641 aggccgagct gttcggcggc gcgggtcttc gagccgcact cgcgcagggc gtcctcgatc
     2701 atccggcgct tgagggcttc caccgcctcg ctgagcgtgc gcaccgacgg ggcggcaccc
     2761 gcgccggccg cgatgcgctc cgagaggtgc gcggggccga gcgggcgccc gtcgtcggcc
     2821 agggccaccg cgcgctcgat ctcgttctcc agctcgcgca cgttgcccgg ccaggggtac
     2881 ccgcgcagca ggctgagggc ctccggcccc accgcgggca ccgcgcgccg gccccgccgg
     2941 caggcctggc gcaggaagtg ctccgcgagg gtcgggatgt cctcggcccg ctcgcgcagc
     3001 ggtgggaggc ggaccgggaa gacgctcagg cgatagaaga ggtcgcgacg gaaccggccg
     3061 gcggccacgt cggcctcgag atcggcgttg gtggccgcga tcagccgcac gtccacccgg
     3121 cgcgtccccg agccgccgac ccggcgcacc tcgccctcct gcagcacgcg cagcaggcgc
     3181 agctgcatcg cgggcgatgt ctcgccgacc tcgtccagga agatcgtgcc cccgtcggcc
     3241 tcctcgaaga ggcccttgcg ctcgcccacc gcgccggtga aggcgccgcg ggcgtggccg
     3301 aacagctccg actcgagcag cgtgtcgggc agcgccccgc agctctgggc cacgaacggg
     3361 cgggtgcggc gggaaccgtt ggtgtggatg aggtgggcga agagctcctt gccggtgccg
     3421 gtctcgccga gcagcagcac gctggtgtcg ccgtcgagga ccttgcgcgc cagctcgacc
     3481 gcgccgcgca gcccggtctc cgcgccgatc agcttgtcga acgacgccgg cttctcgcgg
     3541 ccggcggtgg cctcgcgccg gagcgcgttg taggccagct cgagctcgtc gcggagccgg
     3601 gcgttctccc gggccagccg ccaccgctcc gcggcccggc gcaccaccac cagcagctcg
     3661 ttcgggtccc agggcttggg gacgaagtgg tagatccggc cggtgttgat cgcctccatc
     3721 aggctgtcga cgtcggtgta cgcggtcagc acgatccgga ccgcgtccgg cgtccgctcc
     3781 tggctgcgtc gcagcagctc gaccccggtc atgccgggca tccggtggtc ggtcacgatc
     3841 accgccacgt ccgcctcggc ggcgaggcgc tcgagcgcct cctcgccgtg gttggcgcgg
     3901 agcacgcgga actcggccgc gaggatcgcc tcgagcgcgt cgagcacccg cggctcgtcg
     3961 tccacgatca ggatcgtggt ctcgtccgcc gagcctccgg gcgaaggggc cgtcacgtcg
     4021 aggccggaac cgcgaccgcc ggctcccgca ggcggcagat ctcgtagagc gtctggcgct
     4081 ggcggatgcc cttgaggtgc ggccggccga gctcgcgggc gtcgacgaga tggttcacct
     4141 ggtggaaggt ctcctcgccc acgatgatat gcgcgtcgaa gaggcgtgtc atccgctgca
     4201 ttcgcgacgc cgcgatcatc gcgcggccca ccacgatgag ctcgcgccgg cggtccgagc
     4261 cgatcgtccc cgccagcacc cgaccggagc tgatgccgat cccgatgtcg aaggtgggcc
     4321 gcccgtcggc ggtccagcgg gcggccagcc gccgcactgc ctccaccatg tcggcggcgg
     4381 cccggaccgc ccgcagcgcg acgtcggccc ccggctccgg gacgccgaag agggcgacga
     4441 tctcgtcgcc gaccagctgc tccaccgtgc cctggtggcg gaagatcacg tcgatcatct
     4501 cggtgaagta ctcgttgagg agctggatca ccaccggggc ggtcgtcgac tcgatgaggc
     4561 gggtgaagcc gcggatgtcc gcgaagagca cggtggcctc gcgccagtcg cccggcagct
     4621 ccaggatgcc gggattggcc accgcgaggt cgaccagccg gggcgacacg tagcgctgga
     4681 gctgcgagcg caccgacgcc tcccggatca ggtcgtcctg ggcgtcgcgc acgtgggtga
     4741 gggcctgctc cagctcggca ttcttggccg cgagattccc cagcaacgtc tcgcgctccc
     4801 ggcagagccg gtgatgctcg accgcccggc ccaccacgtg acggagctcg ttcgggtccc
     4861 acggcttcag gaggaagtgg tagaccccgg ccgcgttgat ggagtccatc agggcttcgg
     4921 tgtcggtgaa ggcggtgagg aggatgcgga ccgtgtcggg cgccacgtcg cggctccggg
     4981 tcaggagctc ggtgccggtc agcccggcca tgcgctggtc gctcaccacg acggccacgt
     5041 cctcctcgcg gaggtgacgg agggcgatct cggggcgctc cgcgcgaagg atgcggtgct
     5101 ccatcgcgag gagcgcctcg agcgcgtcga ggacgcgcgg ctcgtcgtcg accaggagga
     5161 cggtggcggg tgtcatggga ctcgcggggc ggcggccggc gtcgtctcgg cgggcttgcc
     5221 ggcccgggcc accggcagcc gcagggtgaa caccgcgccg ccgtcgaccg ggctctcggc
     5281 ccggatctcg ccgccgtgct tcgtgacgat ctcgtagctg atcgagaggc cgagcccggt
     5341 gccctcgccc accggcttgg tggtgaagaa cggatcgaag acgcggcgga gcacgtcggg
     5401 cgggatgccg aggccggtgt cggcgatggc gatctccgcc atcggcccca cgcggcgggt
     5461 ctcgatggtg atcgtgccgg gtccggggat cgcctgggcc gcgttggcga gcacgttcag
     5521 gaagacctgg tcgatctgcg accggacgca ctccaccggg ccgagcgagc cgtacttgcg
     5581 gatcaccgtg acccgatcct tcagcaggtg gttcaggagg gtcaggctcg aatcgagctc
     5641 ctcgtgcagg tcgaccgcct gccacacgtc gtcgggggtc cgggtgaaga cccgcaggtc
     5701 gccgacgatc ttgcgggtcc ggtcggcgcc ctcgcggatg cccgagatca tcgagtcgag
     5761 gtaccggagc gcgtagtcga cctggagctc gcgccggcgc gcgtcgaccc gggcccggtc
     5821 cgcctcgggc agcgcggcct cctggtaggt cgcgagcatc gcccgcaggc gctggacgaa
     5881 gtcggccagc gtggccacgt tggaggagat gaacccgatg ggattgttga gctcgtgggc
     5941 cacgccggcc acgagctggc cgaccgaggc catcttctcg cgctggatga gctgcagctg
     6001 cgtggtctgg agatcgcggt aggcggtccg cagctcctcg ttggcggccc ggagctgctc
     6061 ggtgcggacg cgcaccttgt cctcgaggct ccgggagagg tcctcgatgg tctggaagct
     6121 cgcggcccgg tcgatggcga gggccacgtg gctggccacc gcggtgagca ggtccacgtc
     6181 gccccggtcg aagcgtccgg cctccgtcga ggtgaccgag agcacgccga gcacctgctc
     6241 gcgcacgcgg agcggcgccg ccacgaggct ccgcacccgg tgggctcggc tcagcgcgtg
     6301 gtgcaccggc tcggtcgccg tctccacgtc gctgacgagc accggctgcc cggtgatcgc
     6361 ggcccgcgcg ctcgcgctgc tcgtcgcctc gagggggagc tcgaccccct cgaacggggg
     6421 cgccgcgggg tcggcgcccg cggagcggtg cccgcggagc acgcggcgct cgcggtccac
     6481 caggaggagg taggcccgct cgtacggcat ccggtggacg agccggctca gcgcctggtc
     6541 gtagatcttc tccgggtcga gcgtcccgct caccgcggcg ccgaggtcgg tgaggagcga
     6601 gagctgctcg atccgcgtct cgagctcgac gaacttcttc tccagctcgc ggttcgagta
     6661 gatgatctcc tcggactgga gatcgagcag gcgctgggtg tggcggcgct gctggccctc
     6721 gcggagggcg tagccgagcg cgacgcccgc gaggaggggc aggggcgacg cggccaggag
     6781 cggccagctc ccgcccggcg aggcggcgac cagggcgagg agggcggcag ccgcgccgcc
     6841 gatcaggctc ggcgcccaga agcggggccc ccgcgacgga ttcttccagc ggacgtccca
     6901 gcggcaggcg tcgccgccgc gggccgcgca cgtggtctcg agaatgtggg cgcgcggcaa
     6961 tccccagttg gtcgggaagc gctcgaactg gaccttgcgc cacgtgcagc tccagaccgg
     7021 gatggtctgc ccggccatcg gcgtccagcg gtaacgggcg tggcggcggc cgacctcgag
     7081 cgtgtcgagc cggtagaggc cgcggctctg ctgctcgtag atggtggaca cccgctggta
     7141 ggccgcgcgc gggttgccga tgcccatgga gtaggtgccg agatacgagc gctcctcgcg
     7201 gggcttccag tccatgaagg actcgccgta gcgccgcgcc cagcggtcct cgtcgggctc
     7261 gcgcatgag
//
LOCUS       DATMCV010000256         2452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_480318, whole genome shotgun sequence.
ACCESSION   DATMCV010000256 DATMCV010000000
VERSION     DATMCV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480318"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VNU03_05945"
     CDS             <1..1187
                     /locus_tag="VNU03_05945"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWP74033.1"
                     /translation="ERVIGASPSREEAVRILQALGFAVDDSGDDLQVVVPSFRRDIHQ
                     EDDLVEEIVRIWGYDKIPLILAGGGEIIPVKRPAGLRVARVMSRALNAAGLYECVTWA
                     FLDPDRLKRMGWSDRDALIALQNPLSVERSILRPSLLPGLLEILAINANRQMPDARCF
                     EVGNVFAPHRPEDGDRPAHEELRLGLVLTGLRAPRAWFTGGRDRVDVYDAKGIAEAAL
                     AAAGVTDAEAVLWPDDQAPAYLEPGRAARLVRGSADLGWFGEVALAAREAFDLPGGVF
                     AAELSVTALAAQPPAGVRYEPLPRFPAVQRDLAIVVSAEVTAGQVEAAIRAMNAPLLT
                     RVTLFDVYTGGQVGAGRRSLAWSLTFQAPDRTLTDAEVNRVHEQIVAEVARRFRAEVR
                     GA"
     gene            1184..1444
                     /gene="zapB"
                     /locus_tag="VNU03_05950"
     CDS             1184..1444
                     /gene="zapB"
                     /locus_tag="VNU03_05950"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HWP74034.1"
                     /translation="MTEERMAEGLTERLEQLEKSVRRAAETITRLRKERDSLQAKVVG
                     LEAKLGAAEGDRAELASLRQERKEVLSQVDGILKELDRLDIQ"
     gene            1463..1765
                     /locus_tag="VNU03_05955"
     CDS             1463..1765
                     /locus_tag="VNU03_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HWP74035.1"
                     /translation="MAEKQRVDIEILGQKYPIRSEAGPDYVRQLASFVDRRAREIRGD
                     AAGQDAVRVLALTALDITDELFRLRDERKEQDIDHKDVTARLGALRQLLDAVVPEK"
     gene            1807..1989
                     /gene="ssrS"
                     /locus_tag="VNU03_05960"
     ncRNA           1807..1989
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VNU03_05960"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2191..>2452
                     /locus_tag="VNU03_05965"
     CDS             2191..>2452
                     /locus_tag="VNU03_05965"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74036.1"
                     /translation="MAVLALLVGYFGSRAATARTLGDAETRASRILEDAKRLVDQARS
                     DADGKVREADTKVREAEAKIRGADLEAKELVLKVRGELDQESR"
BASE COUNT          371 a          837 c          892 g          352 t
ORIGIN      
        1 tcgagcgtgt catcggagcg agcccgtcgc gcgaggaggc ggtgcgcatc ctgcaggcgc
       61 tcggcttcgc ggtggacgac tcgggcgacg atctgcaggt ggtggtgccg agcttccggc
      121 gcgacatcca ccaggaggac gatctcgtcg aggagatcgt ccggatctgg ggctacgaca
      181 agatccccct gatcctggcc ggaggcgggg agatcatccc ggtcaagcgc cccgccgggc
      241 tccgggtggc ccgcgtgatg agccgcgcgc tgaacgcggc gggcctctac gagtgcgtga
      301 cgtgggcctt cctcgatccg gatcggctca agcggatggg ctggagcgat cgggacgcgc
      361 tcatcgcgct ccagaacccg ctctcggtgg agcgctcgat cctgcgcccg tcgctgctgc
      421 ccggcctgct cgagatcctg gccatcaacg ccaaccgcca gatgccggac gcgcggtgct
      481 tcgaggtcgg caacgtcttc gcgccgcacc ggcccgagga cggcgatcgg ccggcccacg
      541 aggagctgcg gctcggcctc gtgctcaccg ggctccgggc cccgcgggcc tggttcaccg
      601 ggggacgcga ccgagtggac gtgtacgacg cgaaggggat agccgaggcg gccctcgccg
      661 cggccggcgt caccgacgcg gaggccgtgc tctggcccga cgaccaggcg cccgcctacc
      721 tcgagccggg gcgcgccgcg cgcctcgtgc ggggcagtgc cgacctcggc tggttcggcg
      781 aggtggcgct cgccgcccgc gaggccttcg acctgccggg cggggtcttc gcggccgagc
      841 tgtcggtgac cgcgctggcc gcgcagccac ccgccggcgt gaggtacgag ccgctcccgc
      901 gcttccccgc ggtccagcgc gatctcgcga tcgtggtgag cgccgaggtg accgcgggcc
      961 aggtcgaggc cgcgatccgc gcgatgaacg cgcccctgct cacccgcgtg accctgttcg
     1021 acgtgtacac tggcggccag gtcggggcgg gtcgccgcag tctcgcctgg agcctcacgt
     1081 tccaggcgcc cgatcgcacc ctcaccgacg ccgaggtgaa ccgggtacac gagcagatcg
     1141 tcgccgaggt cgcgcggcgg ttccgggccg aggtccgcgg cgcatgacgg aggagcggat
     1201 ggcggaaggg ctgacggagc gtctggagca gctcgagaag tcggtgcggc gcgcggccga
     1261 gaccatcacg cgcctgcgca aggagcgcga ttcgctgcag gccaaggtgg tgggcctcga
     1321 ggccaagctg ggggccgcgg agggggatcg ggccgagctg gcgagcctgc gccaggagcg
     1381 gaaggaggtg ctgtcccagg tggacggcat cctgaaagag ctggaccggc tcgacatcca
     1441 atagccgcag gaaggggacg ccatggcaga gaagcagcgg gtcgacatcg agatcctggg
     1501 gcagaagtac ccgatccgca gcgaggccgg ccccgactac gtgcgccagc tggcctcctt
     1561 cgtcgaccgc cgggcgcgtg agatccgggg cgacgcggcc gggcaggacg cggtccgggt
     1621 gctggccctg accgcgctcg acatcaccga tgagctgttc cgcctccgcg acgagcgcaa
     1681 ggagcaggac atcgaccaca aggacgtgac cgcccgcctc ggcgcactgc ggcaactcct
     1741 cgacgcggtg gtgcccgaga agtaacgatt cgcttgaccc gcccacgggg ccgggggtac
     1801 acaagagagg ccctgcactg ttcgtgatgc cggggggaag tttcaacctc gtttaaagat
     1861 tgggagccgc tcacgagggt ggtgcgcatg cccctccaag aggggaagcc tgaagccctc
     1921 cattttggcg cccacctggt gcataccagg ttcgaaacac cgtcggcaca cggcagaggt
     1981 ggggcacctc cttctcgccg cgcttcctcc cgtagcactt ctgcagcact cccgcgttcg
     2041 ttgacattcc acttcgcagc ggcctataat tcgtcaactt tagcggctat ttgccgcacg
     2101 caaccccgag tgcacacgat ccggaccggg cctcgtcccg ggatggagtg atcgatgatt
     2161 tctatgccgt ggctggtggt gcttccgcta gtggcggttc tcgccttact ggtcggatac
     2221 ttcggctccc gcgcggccac cgcgcgaacg ctcggcgacg ccgagacgcg tgcctcccgc
     2281 attctcgagg acgccaagcg cctggtcgat caggcgcgct ccgacgccga cggcaaggtg
     2341 cgtgaggccg acaccaaggt ccgcgaggcc gaggccaaga tccgcggcgc cgacctcgag
     2401 gccaaggagc tggtcctcaa ggtccggggc gagctggatc aggagtcccg gg
//
LOCUS       DATMCV010000257         4262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_614794, whole genome shotgun sequence.
ACCESSION   DATMCV010000257 DATMCV010000000
VERSION     DATMCV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614794"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VNU03_05970"
     CDS             complement(<1..267)
                     /locus_tag="VNU03_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP74037.1"
                     /translation="MLEVRDLSVAYGGLRALERVTLSVAEGRFVTLVGPNGAGKSTLF
                     KTISGVVTPVSGSISFAGEDLLARRASDRAHLGIAHVPEGRQVFK"
     gene            complement(260..2038)
                     /locus_tag="VNU03_05975"
     CDS             complement(260..2038)
                     /locus_tag="VNU03_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HWP74038.1"
                     /translation="MKQGAGPHLRRHLTVLAVVSVVYLVVAMFVTNSYYQLILTLVPV
                     WALFGVSWNILSGYGGQLSFGHASFFGIGAYTVTLAVIYWGLSPWLGIPLGMVIGGLA
                     AIAIGFPTFRLRGHYFALSMLAYPLAILYVMQYLGFQEVSMPMHRENGLRFMEFSEPR
                     IYTLLAVLLLVGGVVVSLIIENSRFGLALLAIRQNELAAEAAGINARLWKMRSLVVSG
                     MMGAAAGGFYARVLLVVTPDSVFGMLVSAQAVVVTLFGGVASVWGPVIGAAILVPLAE
                     TLQAELGTYLPGIQGVVYGIAIIAIMLLSPDGLFWTVRDRWFRREAPQPIPDVEPDPV
                     PLPAFKRRASTGVAGPLLQVEGLSRAFGGLRAVSEVSFSVAEGEILGIIGPNGAGKTT
                     LFNVLNGVLTADEGAARLDGQPMLGKKTFEVCRLGVGRTFQVVRSFPRLPVLDNVIVG
                     AYGAGLSDREAIAQAVRALHQVGLHDRSGVAAGQLTNKQLRLMELARALAGSPRLLLL
                     DETLAGLGREECDDVLGVLARVRAQGMTICIIEHTMHAMMRLADRFVVLDHGRVLATG
                     LPRVVVEDTSVIEAYLGKKWLARRDA"
     gene            complement(2035..2991)
                     /locus_tag="VNU03_05980"
     CDS             complement(2035..2991)
                     /locus_tag="VNU03_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74039.1"
                     /translation="MDLGILVENVLNAVTAGITIGCIYGLMCIGLGLIFGIMRIVNFA
                     QGELLMLGMFASFYLVSEGRVLSFLGPYWGPFIGAMCAAPLLFSGGALLHKFLISRVS
                     GLRTVGSLDEGHFGQLVVTLGISLILQNGGQILFGSTPVAVRTPLSSQAFQIGPIYQG
                     ATVFLNKAKLVAFFVAVLVAAVLYVTLEYTRLGKALRAAADNPTAATYMGIDVDRAYR
                     IAFGLGCGITAVAGGLMATYISFGPFVGFDYVIIMYSGVVLGGMGSIFGAFWGGVTVG
                     FVQQFSALILPPQLQNAAIFVVFLMIVLLRPQGLFGRSAERA"
     gene            complement(3199..3669)
                     /locus_tag="VNU03_05985"
     CDS             complement(3199..3669)
                     /locus_tag="VNU03_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74040.1"
                     /translation="MHHDASSAPLAQEVRRATDRYQDVSAATAAGYALFLGCVSSPTG
                     GAMGIHYVNGDLVGDGKIDAARPEALIYEPKGGKLELVGVEYIVIAAAWDAANKTPPT
                     LMGQLFHLNSAPNRYGIPAFYALHVWAWRHNPQGTFVDYNPRVSCDGYPTEAAR"
     gene            complement(3770..4042)
                     /locus_tag="VNU03_05990"
     CDS             complement(3770..4042)
                     /locus_tag="VNU03_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HWP74041.1"
                     /translation="MPRYLVERTFPDGLAIPLSEAGAQAVQGVVAINAEQLVTWVHSY
                     VTADRKHTFCVYDAPTPEAIRQVAERNKLPVDRITEVRVLDPYFYR"
BASE COUNT          741 a         1479 c         1477 g          565 t
ORIGIN      
        1 cttgaacacc tgccggcctt ccgggacgtg cgcgatgccc agatgcgcgc ggtccgacgc
       61 ccggcgggcc agcaggtcct cgcccgcgaa cgagatggag ccggagacgg gcgtgacgac
      121 cccgctgatc gtcttgaaca gggtggactt gccggctccg ttcgggccga ccagggtgac
      181 gaagcgaccc tccgcgacgg acagggtcac gcgctcgagg gcgcgcagcc caccgtaggc
      241 gacggagagg tctcggacct caagcatcgc ggcgcgccag ccatttcttg ccgaggtagg
      301 cctcgatcac ggaggtatcc tccacgacga cccggggcag cccggtcgcg agcacgcggc
      361 cgtggtccag caccacgaac cggtccgcga ggcgcatcat cgcatgcatt gtatgctcga
      421 tgatgcagat cgtcatgccc tgcgcgcgca cccgggccag cacgcccagg acgtcgtcgc
      481 actcctcccg cccgagcccg gccagcgtct cgtccagcag caggagccgc gggctgccgg
      541 ccagcgcgcg ggccagctcc atcaggcgca gctgcttgtt ggtgagctgg ccggcggcca
      601 cgcccgaccg atcgtgcaat cccacctgat gcagcgcccg cactgcctgg gcgatcgcct
      661 cccgatcgga caggccggct ccgtaggcgc cgacgatcac gttgtcgagc acgggcaggc
      721 gcgggaagct gcgcacgacc tggaacgtgc ggccgacgcc caagcggcag acctcgaagg
      781 tcttcttgcc cagcatgggc tgcccgtcga gccgggccgc cccctcgtcg gcggtgagca
      841 cgccgttcag cacgttgaac agcgtcgtct tccccgcgcc gttcggtccg atgatgccga
      901 ggatctctcc ctcggcgacc gagaagctga cctcgctgac cgcgcggagc ccgccgaagg
      961 cgcgcgagag cccctcgacc tgcaggagcg gccccgccac gccggtcgac gcgcgccgct
     1021 tgaaggccgg gagcggcacc ggatcgggct ccacgtccgg gatcggctgc ggcgcctccc
     1081 ggcggaacca gcgatcgcgc accgtccaga atagtccgtc gggcgagagc agcatgatcg
     1141 cgatgatggc gatgccgtag accactccct ggataccggg caagtaggtg cccagctcgg
     1201 cctgcagcgt ctccgcgagc gggaccagga tggcggcgcc gatcaccggc ccccacaccg
     1261 aggccacacc gccgaacagc gtgaccacca ccgcctgggc ggatacgagc atgccgaata
     1321 ccgaatccgg cgtcaccacc agcagcacgc gcgcgtagaa gccgccagcc gccgcgccca
     1381 tcatccccga caccaccagc gagcgcatct tccagaggcg cgcgttgatg cccgccgcct
     1441 ccgcggccag ctcgttctgg cggatggcga gcaacgccag tccgaagcgc gagttctcga
     1501 tgatcagcga gacgaccacc ccgccgacca gcaggaggac cgcgagcagc gtgtagatgc
     1561 gcggctcgct gaactccatg aagcggagcc cgttctcgcg gtgcatcggc atcgacactt
     1621 cctggaagcc gaggtactgc atcacgtaga ggatggcgag cggataggcc agcatcgaca
     1681 gcgcgaagta gtgcccgcgc aggcgaaacg tggggaagcc gatcgcgatg gcggcgagcc
     1741 cgccgatgac catgccgagc gggatgccga gccacggcga gaggccccag tagatgaccg
     1801 cgagcgtgac cgtgtacgcg ccgatgccga agaacgacgc gtggccgaag gagagctggc
     1861 cgccgtagcc ggagaggatg ttccacgaga cgccgaagag cgcccacacc ggcaccaggg
     1921 tcaggatcag ctggtagtag ctgttggtca cgaacatcgc gaccaccagg tagacgacag
     1981 agacgacggc gagcaccgtc aggtgccgcc gcaggtgggg gccggcgccc tgtttcatgc
     2041 ccgctccgcc gagcggccga acagaccctg cggccgcagc agcacgatca tcaggaacac
     2101 gacgaagatc gccgcgttct ggagctgggg cggcaggatc agggccgaga actgctggac
     2161 gaagcccacc gtcaccccgc cccagaacgc gccgaagatg ctccccatgc cgcccagcac
     2221 cacgcccgaa tacatgatga tgacgtagtc gaagccgacg aagggaccga aggagatgta
     2281 cgtcgccatc aacccgcccg cgaccgccgt gatgccgcac cccaggccga aggcgatgcg
     2341 gtaggcccgg tccacgtcga ttcccatgta ggtggccgcc gtcgggttgt ccgccgccgc
     2401 ccgcagggcc ttgcccaggc gcgtgtactc gagcgtcacg tagagaaccg ccgcgaccag
     2461 gaccgcgacg aagaaggcca ccagcttggc cttgttcagg aacacggtgg cgccctggta
     2521 gatcgggccg atctggaatg cctgcgagga gagcggcgtg cgcaccgcga ccggcgtgga
     2581 gccgaacagg atctgcccgc cgttctgcag gatcagcgag atccccaggg tgacgacgag
     2641 ctggccgaag tgcccctcgt ccagagagcc cacggtgcgc aggcccgaca cgcgcgagat
     2701 gaggaacttg tgcaggagcg cgccgccgct gaagagcagc ggggccgcgc acatcgcgcc
     2761 gatgaacggc ccccagtacg ggcccaggaa ggacagcacc cgcccctcgc tgaccagata
     2821 gaaggaggcg aacatgccca gcatgagcag ctcgccctgg gcgaagttga cgatccgcat
     2881 gatgccgaag atgagcccga ggccgatgca catcagaccg tagatgcaac cgatcgtgat
     2941 gcccgcggtg accgcgttca gcacgttctc gacgaggatt cccaggtcca ccgtgcttgc
     3001 gcctacctcc cgtgggcggc ggccgggggg gctggcgcgc ccttgcgcag cgcgcctccc
     3061 ggacacagcc cggcgacttt accacgtccg cggcgagcgc gaggcaaacg gggagcgggt
     3121 ggtcaggcgg agggagccgg ccggggggcc ggccggctcg ggctcgttct tcgcgagcgg
     3181 gctgctacga gctgccgctc agcgtgccgc ctcggtcggg tagccgtcgc aggacacgcg
     3241 cgggttgtag tccacgaacg tcccctgcgg gttgtgccgc cacgcccaca cgtgcagcgc
     3301 gtagaaggcg ggaatgccgt agcggttggg ggccgagttg aggtggaaga gctggcccat
     3361 cagcgtgggc ggcgtcttgt tggccgcgtc ccaggcggcc gcgatcacga tgtactccac
     3421 gcccaccagc tcgagcttgc cgcccttcgg ctcgtagatc agggcctcgg gacgcgcggc
     3481 gtcgatcttg ccgtcaccga ccaggtcgcc gttgacgtag tggatgccca tcgccccgcc
     3541 cgtcgggctg ctgacgcagc cgaggaagag cgcgtagccg gcggcggtgg ccgccgagac
     3601 gtcctggtag cgatcggtgg cccggcgcac ttcctgggcg agcggcgccg acgaggcatc
     3661 gtggtgcatg ccgcccgacg agcacgagac gagaaagact gcggagagtc cgatcaacac
     3721 gcgactcgat gtcttcactg gaggctcctt ggtctcaacg ggattggtct caccggtaga
     3781 agtagggatc gagcacgcgc acctcggtga tgcggtcgac cggcagcttg ttgcgctcgg
     3841 ccacctggcg gatcgcctcc ggggtcggcg cgtcgtacac gcagaaggtg tgcttgcggt
     3901 cggccgtgac gtaggagtgg acccaggtca cgagctgctc ggcgttgatc gcgacgacgc
     3961 cctggacggc ctgggcgccg gcctccgaga gcgggatcgc gagaccatcg gggaaggtcc
     4021 gttccacgag atagcggggc atatggtgat gctcctttcc gccgcgagag ctcgatggct
     4081 cggccgcggc atcctcgcgt cgagatgtac cagcgccccg tcaccgcacc gtcaccggcg
     4141 ccgtctattc accgtcaccc agccccacgg cgcggtgcta tagtcggcag acgacggtgg
     4201 catggcgatg acgcagctct cgcttcgact cctcggtggg ctccgcgccc agcacggctc
     4261 cg
//
LOCUS       DATMCV010000258         2220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_614797, whole genome shotgun sequence.
ACCESSION   DATMCV010000258 DATMCV010000000
VERSION     DATMCV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614797"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VNU03_05995"
     CDS             complement(<1..907)
                     /locus_tag="VNU03_05995"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWP74042.1"
                     /translation="MEVPFLDLTRQHRRVRDEIGAAITRTLERGRYVLGEEVAGFERE
                     FAAYCGARLAVGVGSGTDALHLALRAAGVQAGDRVITAPNIAVPTVCAIVAAGAVPVF
                     VDVDPRTCTLDPEKLRAHLEASPPTRRPRAVIPVHLYGHPAALDEIAQICAEHDLTLI
                     EDAAQAHGAEYAGRRIGAHAAFSCWSFYPTKNLGAYGDAGMVVTDDAQGAERVRMLRN
                     YGEEAKYRNRVPGVNSRLDELQAAMLRVKLRHLDEWVSARRRLASLYDRLLVDAPVTR
                     PVEVGRARHGYHLYVIRSPRRDALQQ"
     gene            complement(913..1908)
                     /locus_tag="VNU03_06000"
     CDS             complement(913..1908)
                     /locus_tag="VNU03_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWP74043.1"
                     /translation="MTAALDCFRDRRVLITGGLGFLGSNLAIRLVGLGARVTLLDAMV
                     DDHGGNPFNVEPIRDQVAVNFSDIRDEASLKYLVTGQDYVFHLAGQNDHVLSLRDPFP
                     DIDINIKGSAMLLEACRRFNRSARLVYGGTRGEYGPAVMLPVAEDHPMRPRGIYELSS
                     LTAQQLFQIYHDNHGVASVTLRLTNIYGERAQMKHDRFGVANWFVRQALDDGTIKVFG
                     DGSLLRDFVYVQDAVEAMVQCAATPDAFGQVFNVGNNRASSFRELAETVVRVAGNGRW
                     ELAPFSPERAAQEPGDFCSDIRKIKRVVGWEPTTPLGEGLDRTVGYYRLHRHRYW"
     gene            complement(1908..>2220)
                     /locus_tag="VNU03_06005"
     CDS             complement(1908..>2220)
                     /locus_tag="VNU03_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP74044.1"
                     /translation="VNFSVMPLRVSTVVGLACSVLGLALGVEVLVERLLRPDIPVGWA
                     SVLMPVVLFSGVQLVMLGLLGEYLGRLFLTENQTPQFVVREVVEAGAVVPASSSPEPR
                     P"
BASE COUNT          341 a          794 c          781 g          304 t
ORIGIN      
        1 gctgctggag tgcgtcccgc cggggcgagc ggatcacgta gaggtgatag ccatgacgcg
       61 cccgcccgac ctcgaccggc cgcgtcaccg gggcatccac caggagccgg tcgtagagtg
      121 aggcgagtcg gcggcgcgcg ctgacccact cgtcgagatg gcggagcttc acccgcagca
      181 tcgcggcctg gagctcgtcg agccggctgt tgactcccgg cacgcggttc cgatacttgg
      241 cctcctcgcc atagttgcga agcatgcgca cccgctcggc cccctgggcg tcgtcggtca
      301 ccaccatgcc cgcgtcacca taggccccga ggttcttggt cgggtagaag ctccagcagg
      361 agaaagccgc gtgcgccccg atccgccggc ccgcgtactc ggcgccgtgc gcctgtgcgg
      421 cgtcctcgat cagggtgagg tcgtgctcgg cgcagatctg cgcgatctcg tccagggcgg
      481 cggggtggcc gtagaggtgc accgggatga ccgcgcgggg ccgccgcgtg ggcggcgagg
      541 cctccaggtg ggcccggagc ttctcgggat cgagggtgca ggtacgcggg tcgacgtcga
      601 cgaagaccgg caccgcgccc gcggccacga tggcgcagac ggtgggcacc gcgatgttgg
      661 gagcggtgat cacccgatcc ccggcctgca ccccggcggc gcgcagggcc agatggagcg
      721 cgtcggtccc cgagcccacg ccgaccgcga ggcgggcgcc gcagtaggcg gcgaactcgc
      781 gctcgaagcc ggccacctcc tcgcccagga cgtaccgccc cctctcgagc gtccgcgtga
      841 tcgccgcgcc gatctcgtcc cggacgcggc ggtgctgccg ggtcaggtcc aggaagggca
      901 cctccatggc ggtcaccagt agcgatgccg gtggaggcgg tagtagccca ccgtccggtc
      961 caggccctcg ccgagcggcg tggtgggctc ccagccgacc acgcgcttga tcttgcggat
     1021 gtccgagcag aagtcgcccg gctcctgggc cgcccgctcc ggggagaacg gagccagctc
     1081 ccagcggcca ttccccgcca cccgcaccac cgtctcggcc agctcgcgaa agctggacgc
     1141 gcggttgttg cccacgttga agacctgacc gaaagcgtcg ggggtcgccg cgcactggac
     1201 catggcctcg accgcgtcct gcacgtagac gaagtcgcgc aggagcgagc cgtccccgaa
     1261 caccttgatc gtcccgtcgt cgagggcctg ccgcacgaac cagttggcca ccccgaagcg
     1321 atcgtgcttc atctgggcgc gctcgccata gatgttggtg agcctgagcg tcaccgaggc
     1381 gacgccgtgg ttgtcgtggt agatctggaa gagctgctgc gcggtcaggc tcgacagctc
     1441 gtagatgccc cgcggcctca tcggatggtc ctccgccacc ggcagcatca ccgcggggcc
     1501 gtactccccg cgcgtgcccc cgtagaccag gcgggcggac cgattgaaac gccggcaggc
     1561 ctcgagcagc atggccgagc ccttgatgtt gatgtcgatg tccgggaacg ggtcccggag
     1621 gctcagcacg tggtcgttct gcccggccag gtggaacacg tagtcctggc cggtcacgag
     1681 gtacttgagg ctcgcctcgt cgcggatgtc gctgaagttg accgcgacct ggtcgcggat
     1741 cggctccacg ttgaacgggt tgccgccgtg gtcgtcgacc atcgcgtcga gcagggtcac
     1801 ccgggccccg aggccgacca gccggatcgc gaggttgctg cccaggaacc cgagcccgcc
     1861 ggtgatcagc acccgacggt cgcggaagca gtccagggcg gcggtcatca gggtcgcggc
     1921 tccggcgacg acgaagccgg caccacggcg cccgcctcga ccacctcgcg caccacgaac
     1981 tgcggggtct ggttctcggt caggaagagg cgccccaggt actcgccgag cagaccgagc
     2041 atcacgagct gcacgcccga gaacagcacg accggcatca gcaccgaggc ccaaccgacc
     2101 gggatgtcgg gtcggagcag ccgctcgacg agcacctcga cgcccagggc gagccccagc
     2161 acgctgcagg ccagccccac gaccgtgctc acccggagcg gcatgaccga gaagttcacg
//
LOCUS       DATMCV010000259         5584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_281224, whole genome shotgun sequence.
ACCESSION   DATMCV010000259 DATMCV010000000
VERSION     DATMCV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281224"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VNU03_06010"
     CDS             <1..1058
                     /locus_tag="VNU03_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWP74045.1"
                     /translation="GMRGWLERLGWSRAPERRAMVLAGGGVIGGMYEVGALAALDELP
                     GFRANDFDLYVGSSAGSVVGALMANGVRPAELYAILDEERDDPLNFQKGAVYHKGSFS
                     GAARNLAQFVWAIGKRAVTDFRLEWPDLLARSGGDMPPGFFSLAPLEAYVREALLAKG
                     LSNDFAGTPRPLLIAAIALDQAERVVFGSGDVIDVPISQAIAASSAIPGFFEPYRIRG
                     RDYVDGDVGYTGHADLAVDAGATVLVALNPAVPQCLNGADMAIRKGGLYAIMEQTTHI
                     ASLNLFNVGLREIKLMHPEVQIMVVQPEPVPSPLVGPSMGFEASRAALRYGYRTVKEW
                     LAGPGAEVTARFTQSRS"
     gene            complement(1059..1922)
                     /locus_tag="VNU03_06015"
     CDS             complement(1059..1922)
                     /locus_tag="VNU03_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HWP74046.1"
                     /translation="MTSRVPALIVHGGAGADPGGRDELRAGMREAMLAGWRALESGGT
                     ALDAVEAAVRSLEDHPRFNAGRGSVLTSEGTVETDASIMEGDRLRNGAVASVSGVRNP
                     ITLARRILEDGHHSLFVGPGAAVRARALGVPLCDPEELITEHQRRRLAAVQAGTVGAV
                     ALDRAGTIAAATSTGGTAGKLPGRVGDSALIGCGTYAESTLGGVSCTGDGEAIIRVAL
                     ARRTLEILKTVGDPTHAAEVAQSVLVEEGRGQGGLIVMDWRGRMGWARSTVLMPVGLM
                     SPGRAEPELPF"
     gene            1955..2659
                     /locus_tag="VNU03_06020"
     CDS             1955..2659
                     /locus_tag="VNU03_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWP74047.1"
                     /translation="MKTDSSRRDLVSLAERITRCRLCPRLVAHREAVGAVPPPRYRGQ
                     VYWARPLPGFGDPRARVLLMGLAPAAHGGNRTGRMFTGDRSGDWLFRALHEAGFANQP
                     TSTHAGDGLRLDGAYITATIRCAPPANKPLPVETAHCRPFLLEELGLLSRVQVVVALG
                     KIGWDAYLRTRRELGLGLPRPLPRFGHGARAVMPGDGVTLLGSFHPSQQNTFTGKLTR
                     PMLRAVFRQARQLADR"
     gene            2703..4076
                     /locus_tag="VNU03_06025"
     CDS             2703..4076
                     /locus_tag="VNU03_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWP74048.1"
                     /translation="MVGMPRSHVLSLWTLVFCLGLAAHAAAQPATTGPARLAVTEATL
                     DNGLRVLIQEDPRNPIVAVQMFYRVGSRNELPGATGLAHFLEHMMFKGTPTRGRGEIS
                     RLIELNGGRDNAFTTKDMTGYHVNIAADRLDLVLTIEADRMRNLLLAPAEIDSERKVV
                     MEERRSRIDDDPEGVVYEAMSSVAFLAHPYRWPIIGWMSDIARINPAELRAFYDAYYQ
                     PNNAILVIAGDVKAPAALALVRRHFGPIPRGANPAPVTALEPAQSDERRVIVRKEDAQ
                     LPVVNIAWHVPNFRSDDAPALEVLSTILSEGRASRLYRHLVYEKRMALGAGGEYSYSA
                     LDPTLFWFYATLLPGQTPEAVEQALLGEVEHLKKEPVPAEELERARNQIEASFVWQQD
                     SVFSRASILGRFEMLGSWRLLDDYLPRIRKVTAADLQQVARRYFLVDRKNISILLPAE
                     PAAAAAR"
     gene            4073..5398
                     /locus_tag="VNU03_06030"
     CDS             4073..5398
                     /locus_tag="VNU03_06030"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWP74049.1"
                     /translation="MRRPRRLWAVAAALALDVVLAPMAVTPAQAVGPLARREVLPNGA
                     VLLVAERPAIPIVVVRLSVPAGAVRDPADGLGLANLTADMLTRGTARRSGPALDQAIE
                     FVGGSLEADAGRDSATITLSVLKKDLTLGLDLLAEVLRQPTFPADELSRKIADIQASL
                     RRSEQSPETVAGRVLGPLIFPGHPYGQPAAGTVESVGKLTREQVVAFHRLHYRPDGAN
                     IVVVGDVTEPEIRRALLQRLAGWTAPASPLPPVPAPTATVAASSRQITRDLSQTTVFL
                     GRPSIRQDDPDYFPLAVATYALGGGSASRLYMRVREEKGLAYSVYAGAQPGRYGASYL
                     VSLQTRTDAVAEAVRLVREEMARMGREPMAPGELELAKSYLIGSFPLRLDTSGKLARF
                     LGAVEEFGLGLDYPERYKASIARVTAEEAQRVAAKYLDPATFSSVMVGK"
BASE COUNT          738 a         2050 c         2029 g          767 t
ORIGIN      
        1 agggaatgcg cggctggctc gagcgcctcg gctggagccg cgcgcccgag cggcgggcca
       61 tggtgctggc cgggggcggg gtcatcggcg ggatgtacga ggtgggcgcg ctcgccgctc
      121 tcgacgagct gcccgggttc cgtgccaacg acttcgacct ctacgtgggc tccagcgcgg
      181 gctcggtggt gggcgccctc atggccaacg gggtgcgccc ggccgagctc tacgcgatcc
      241 tcgacgagga gcgcgacgac ccgctcaact tccagaaggg cgcggtgtac cacaagggct
      301 ccttctcggg agcggcccgc aatctcgcgc agttcgtgtg ggccatcggc aagcgggcgg
      361 tgaccgactt ccgcctcgag tggcccgacc tgctcgcccg cagcggcggc gacatgccgc
      421 ccggcttctt ctcgctggct ccgctggagg cgtacgtgcg ggaggcgctg ctggccaagg
      481 ggctctcgaa cgacttcgcg ggcaccccgc ggcccctgct catcgcggcg atcgcgctgg
      541 accaggccga gcgcgtggtg ttcgggagcg gggacgtgat agacgtgccg atctcgcagg
      601 cgatcgcggc ctcctcggcc attcccggct tcttcgagcc gtaccgcatc cgggggcgcg
      661 actacgtcga cggcgacgtg ggctacaccg gccacgccga cctcgcggtg gatgcggggg
      721 ccacggtact ggtcgcgctc aacccggcgg tgccgcagtg cctcaacggg gccgacatgg
      781 cgattcgcaa gggcggcctc tacgcgatca tggagcagac cacccacatc gcgagcctca
      841 acctcttcaa cgtcgggctg cgggagatca agctgatgca tccggaggtg cagatcatgg
      901 tggtccagcc cgagcccgtc ccctcgccgc tggtggggcc gtcgatgggc ttcgaggcga
      961 gccgggccgc cctgcgctac ggctaccgca ccgtgaagga gtggctcgcg gggccggggg
     1021 ccgaggtgac cgcccggttc acgcagtcgc gctcctgatc agaagggcag ctcgggctcg
     1081 gcccgcccgg gcgacatcag gcccaccggc atcagcacgg tcgatcgcgc ccagcccatc
     1141 cggccgcgcc agtccatcac gatgagcccg ccctggccgc gcccctcttc caccagcacg
     1201 ctctgagcca cctcggcggc gtgggtcgga tcgcccaccg ttttcaggat ctcgagcgtg
     1261 cggcgggcga gcgcgacccg gatgatggcc tcgccgtcgc cggtgcagga gacgccgccg
     1321 agcgtgctct ccgcgtaggt gccgcagccg atgagggccg agtcgcccac gcgccccggc
     1381 agcttgcccg cggtgccgcc ggtggaggtg gccgccgcga tcgtcccggc gcggtcgagc
     1441 gcgacggcgc cgaccgtccc ggcctgcacc gcggcgaggc ggcgccgctg atgctcggtg
     1501 atcagctcct ccggatcgca cagagggacg ccgagcgcgc gggcccggac ggccgcgccc
     1561 ggtcccacga agaggctgtg gtgaccgtcc tcgaggatgc gccgggccag ggtgatcgga
     1621 ttgcgcactc cgctgaccga ggccaccgcc ccgttgcgca gccggtcgcc ctccatgatg
     1681 gaggcgtcgg tctcgacggt gccctcgctg gtgaggacgg acccacggcc ggcgttgaag
     1741 cggggatgat cctcgaggct ccggaccgcg gcctcgaccg cgtcgagcgc ggtgccgccg
     1801 gactcgaggg cgcgccagcc ggccagcatc gcctcgcgca tgcccgcgcg cagctcgtcg
     1861 cggccgcccg gatcggcgcc ggcgccgccg tgcacgatca gggcggggac tcgactcgtc
     1921 atgccggaga gcttagcatc cgggtcgggc tcccatgaag acggactcct cgcgccggga
     1981 tctcgtctcg ctcgccgagc ggatcacccg ctgccgcctc tgcccgcggc tggtggccca
     2041 ccgcgaggcg gtgggcgcgg tcccgccgcc gcgctaccgc gggcaggtgt actgggcgcg
     2101 cccgctcccg gggttcggcg atccgcgcgc gcgggtgctc ctgatgggcc tggcgcccgc
     2161 ggcccacggc ggcaaccgga ccgggcgcat gttcacgggc gaccgcagcg gcgactggct
     2221 cttccgcgcc ctccacgagg cgggcttcgc gaaccagccc acgtcgacgc acgcgggcga
     2281 cgggctccgg ctcgacggcg cctacatcac ggcgacgatc cggtgcgcgc cacccgcgaa
     2341 caagccgctc ccggtcgaga ccgctcactg ccggccgttc ctgctcgagg agctcgggct
     2401 gctctcccgg gtgcaggtgg tggtcgcgct cggcaagatc ggctgggacg cctatctccg
     2461 cacgcgacgc gagctggggc tcgggctgcc gcggccgctg ccccggttcg gccacggggc
     2521 ccgggccgtg atgccgggag acggcgtcac gctgctcggg agcttccacc cgagccagca
     2581 gaacacgttc accggcaagc tgactcgccc catgctgcgc gcggtgttca ggcaggcccg
     2641 acagctggcc gatcggtagc acaccgcctg ttgtccgggg ggcccgggcg cgggatgtta
     2701 ccatggtcgg catgccgcga tcacacgtgc tctcgctctg gactctcgtc ttctgcctgg
     2761 ggctcgccgc tcatgcggcc gctcagcccg cgaccaccgg ccccgcgcgc ctcgcggtga
     2821 ccgaggccac cctcgacaac gggctccggg tgctgatcca ggaggacccg cgcaacccca
     2881 tcgtcgcggt gcagatgttc taccgggtgg gctcgcgcaa cgagctgccg ggggccaccg
     2941 ggctcgccca cttcctcgag cacatgatgt tcaagggcac gcccacgcgc gggcgcgggg
     3001 agatctcgcg cctgatcgag ctgaacggcg ggcgcgacaa cgcgttcacc accaaggaca
     3061 tgaccggcta ccacgtgaac atcgcggccg accgcctcga cctggtgctg accatcgagg
     3121 ccgatcgcat gcggaacctc ctgctcgccc cggccgagat cgactcggag cgcaaggtcg
     3181 tcatggagga gcgccggagc cggatcgacg acgatccgga gggcgtggtc tacgaggcga
     3241 tgagctcggt ggcgttcctg gcgcatccgt accgctggcc gatcatcggg tggatgtcgg
     3301 acatcgcgcg gatcaacccc gccgagctgc gcgccttcta cgacgcctac taccagccga
     3361 acaacgcgat cctggtgatc gcgggcgacg tgaaggcccc cgccgcgctc gcgctcgtgc
     3421 gccggcactt cgggccgatc ccgcggggcg cgaatcccgc cccggtgacc gcgctcgagc
     3481 cggcccagag cgacgagcgg cgggtgatcg tgcgcaagga ggacgcgcag ctgccggtgg
     3541 tgaacatcgc ctggcacgtg ccgaacttcc gctcggacga cgcgcccgcg ctcgaggtgc
     3601 tgtcgacgat cctctcggaa ggacgcgcct cgcgcctcta tcgccatctg gtctacgaga
     3661 agcgcatggc gctgggggcg ggcggcgagt actcctactc cgctctcgac ccgaccctct
     3721 tctggttcta cgccacgctc ctgcccggcc agaccccgga ggcggtggag caggcgcttc
     3781 tcggcgaggt ggagcacctc aagaaggagc cggtgccggc cgaggagctg gagcgggcgc
     3841 ggaaccagat cgaggcctcg ttcgtgtggc agcaggactc ggtcttctcg cgcgcgtcga
     3901 tcctgggccg cttcgagatg ctgggctcgt ggcgcctgct ggacgactac ctgccgcgga
     3961 tccgcaaggt gaccgcggcc gatctgcagc aggtcgcccg gcgctacttc ctggtcgacc
     4021 ggaagaacat ctcgatcctg ctgccggccg agcccgccgc ggcggccgcc cgatgaggcg
     4081 gccccggagg ctctgggcgg ttgcggccgc cctcgccctc gacgtcgtgc tcgccccgat
     4141 ggcggtgacg cccgcccagg cggtgggtcc gctggcccgg cgcgaggtgc tccccaacgg
     4201 agcggtgctg ctcgtggcgg agcggccggc catcccgatc gtggtggtcc ggctttcggt
     4261 gccggcgggc gcggtgcgtg atcccgcgga cgggctcggc ctcgccaacc tgaccgccga
     4321 catgctcacg cgcggcaccg cgcggcgctc cggccccgcg ctggaccagg ccatcgagtt
     4381 cgtgggcggc agcctcgagg ccgacgccgg gcgcgacagc gcgaccatca cgctctcggt
     4441 gctcaagaag gacctgaccc tcgggctcga cctgctggcg gaggtgctgc ggcagccgac
     4501 gttccccgcc gacgagctga gccgaaagat cgccgacatc caggcctccc tgcgccggtc
     4561 cgagcagagc ccggagacgg tggcggggcg ggtgctgggc ccgctgatct ttccgggcca
     4621 tccatacggt cagcccgcgg ccggcaccgt cgagtcggtc ggcaagctga cccgcgagca
     4681 ggtcgtggct ttccaccggc tgcactaccg gcccgacggg gcgaacatcg tggtagtcgg
     4741 cgacgtgacc gagcccgaga tccgccgggc gctcctgcag cgcctcgcgg gctggacggc
     4801 gccggccagc ccgctgcccc cggtccccgc gccgaccgcg acggtcgccg cgagcagccg
     4861 gcagatcacc cgcgacctga gccagaccac cgtgttcctc gggcggccct ccatccgcca
     4921 ggacgacccc gactacttcc cgctcgcggt ggcgacctac gcgctgggcg gcggctccgc
     4981 ctcgcggctc tacatgcgcg tgcgggagga gaagggcctc gcctactcgg tctacgcggg
     5041 cgcccagccg ggccgctacg gggcctcgta cctcgtctcg ctccagaccc ggaccgacgc
     5101 ggtggcggaa gcggtgcggc tggtgcggga ggagatggcg cgcatgggcc gggagccgat
     5161 ggccccgggg gagctggagc tggcgaagtc gtacctgatc ggcagcttcc cgctgcgcct
     5221 cgacacctcc ggcaagctcg cccgcttcct gggggcggtg gaggagttcg ggctcgggct
     5281 cgactatccg gagcgctaca aggcgagcat cgcgcgcgtc accgccgagg aggcgcagcg
     5341 ggtcgccgcg aagtacctcg atcccgcgac cttcagctcg gtgatggtgg gcaagtagcc
     5401 gggcggcggc gccccgctca ccaccaccag ccgtacggcc caaagcggga gcgcgggccg
     5461 aagtagtagg ggtcgtaata gtagggcggc ccgtacggcc aggcgcccca gggatagtag
     5521 gcgccgtact ccgcgacctc ctgcggccag aggcggatcc gctccaccgc gatcaccggg
     5581 tacc
//
LOCUS       DATMCV010000260         2566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_947982, whole genome shotgun sequence.
ACCESSION   DATMCV010000260 DATMCV010000000
VERSION     DATMCV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947982"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1909
                     /locus_tag="VNU03_06035"
     CDS             <1..1909
                     /locus_tag="VNU03_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP74050.1"
                     /translation="TYRFNPEVDALAGYRTKSMLVLPMNTPGGDIIGVLELINCKRES
                     GRPFASCEAIERDALPYSQASRNLAASLASQAAVALSNSNLFRELTRRQGRLEALVEV
                     SQRVTRLWPPATVQRRIADLYRGLLQAETVAFHLVDEDRLVRTEARGEGVDVLLPAEI
                     AVGEGPCGGVAASGEPLAIQDLAGSAILNPEQAEMAAKLGLRAWLSVPVRAGDRLVGV
                     LSAVASQSSTCSEDDVAVATTFAAQAGIAVENSRLYAELERALEGVQKSQDQLVQVER
                     LRALGEMAAGVAHDFNNLLAIVMLRTELLLARGQAPEVAESLSLIRQAAQDGAQTVRR
                     IQEFTRTRSIRPFAPVDMNRVIREVVDLARPRWRDQAQSCGVTYDVRIETGKIPLIAG
                     TAEELREAFLNLLNNALDAMPAGGQFTFRTAGEGGRVVVRAEDGGCGMSEETRRRVFE
                     PFFSTKGAQGNGLGLAVVWGIVKRHGGAIEVESALGRGTTFVVNLPVPAELPADGSTR
                     GSTAVPAGKRVLIVEDNPEILQSLADLLRENGCHVIEAADGLAGISRLEAEPVDLVLT
                     DLAMPGASGWEVATACRERFPGTPIGLITGFGDRLEPDKIERHGVQFVVAKPFTSTEL
                     LREVTAALTAS"
     gene            complement(1906..>2566)
                     /locus_tag="VNU03_06040"
     CDS             complement(1906..>2566)
                     /locus_tag="VNU03_06040"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HWP74051.1"
                     /translation="RPMAELSAPPEEPFAFNAGRLCLDFANTVRFRPSSDRLDLIGAY
                     EDLLAWARQATILTPGEAATLVNAAHQGARAAGEALTRARILRESMYAVFAARAAGLP
                     APPADLQTLNRAIARAMAQSGLTAAGARFEWGWPDSPPGLDRVSWWVARSAAELLTSA
                     DLTFVRECASYDCGWLFMDTTKNRSRRWCDMRVCGNRAKSRRHYERRRAAGGSRRPAE
                     R"
BASE COUNT          387 a          890 c          915 g          374 t
ORIGIN      
        1 cacctaccgc ttcaacccgg aggtcgacgc cctcgccggc taccgcacca agtcgatgct
       61 ggtcctcccg atgaatacgc caggtgggga catcatcggg gtgctcgagc tgatcaactg
      121 caagcgcgag agcggccgcc cgttcgcctc ctgcgaggcc atcgagcgcg acgccctgcc
      181 ctattcccag gcgtcgcgga acctggccgc ctccctcgct tcccaggccg cggtggccct
      241 gtccaacagc aacctgttcc gcgagctgac ccggcggcag ggccggctcg aggccctcgt
      301 ggaggtgagc cagcgcgtga cacggctctg gccgccggcg accgtccagc ggcgcatcgc
      361 cgacctctac cgcggcctgc tccaggccga gaccgtggcc ttccacctag tcgacgagga
      421 ccggctggta cggaccgaag cccgcggcga gggcgtcgac gtcctgctcc cggccgagat
      481 cgccgtgggc gagggtccgt gcggcggggt ggccgcctcg ggtgagccgc tggccatcca
      541 ggaccttgcc ggctccgcca tcctgaaccc ggagcaggcc gagatggcgg ccaagctcgg
      601 cctccgggcc tggctctctg ttccggtgcg ggcgggtgat cggctggtcg gcgtgctgag
      661 cgcggtggcc agccagtcgt ccacctgctc ggaggacgac gtggcggtgg ccaccacctt
      721 cgccgcgcag gccggcatcg cggtcgagaa ctcgcggctg tacgcggagc tggagcgcgc
      781 cctcgagggg gtccagaagt cccaggatca gctcgtccag gtcgagcgac tgcgcgcact
      841 gggtgagatg gccgcgggtg tggcccacga tttcaacaac ctgctggcga tcgtgatgct
      901 gcggaccgag ttgctccttg cgcggggcca ggccccggaa gtggccgaga gcctgagcct
      961 gatccgccag gccgcccagg acggcgccca gactgtccgg cgaatccagg agttcacccg
     1021 cacccgcagc atccgaccgt tcgccccggt ggacatgaac cgggtgatcc gggaggtggt
     1081 cgacctggcg cgcccgcggt ggcgggacca ggcgcagagc tgcggcgtga cctacgacgt
     1141 gcggatcgag acgggcaaga ttcccctgat cgcagggacc gccgaggagc tgcgcgaggc
     1201 gttcctcaac ctgctcaaca acgcgctcga tgcgatgccc gcgggtgggc agttcacctt
     1261 ccggactgcc ggcgaagggg gccgggtcgt ggtccgggcc gaggacgggg gctgcggcat
     1321 gtcagaggag acccggcgtc gggtgttcga gcccttcttc agcaccaagg gcgcccaggg
     1381 caacgggctc ggcctggcgg tggtctgggg catcgtgaag cgccacggcg gggcgatcga
     1441 ggtggagagt gcgctcgggc gaggcaccac cttcgtggtc aacctcccgg tcccggccga
     1501 gttaccggcc gacggcagta cccgcggctc gacggctgtc ccggcaggca agcgggtgct
     1561 gatcgtcgag gacaatccgg agatcctcca gtccctggcc gacctcctcc gcgagaacgg
     1621 ctgccacgtg atcgaggccg cggacggcct ggccggcatc tcccggctcg aggcggagcc
     1681 ggtcgacctg gtgctgaccg acctggccat gcccggggca tcgggctggg aggtcgcgac
     1741 tgcctgccgc gagcgattcc cgggcacccc catcgggctc atcaccggct tcggtgatcg
     1801 gttggagccc gacaagatcg agcggcacgg cgtccagttc gtggtcgcca agccgttcac
     1861 atcgaccgag ctgctgcgcg aagtcacggc agctcttaca gcgagctagc gttcggcggg
     1921 ccggcggctc ccgccggcgg cccgccgacg ctcgtagtgg cgcctgctct tcgcccggtt
     1981 cccacagaca cgcatgtcgc accatcgacg gctccggttc ttggtcgtgt ccatgaagag
     2041 ccatccgcag tcataactgg cgcactcgcg aacgaaggtt agatccgccg aggtcagaag
     2101 ctcggcagcg gagcgcgcca cccaccaaga gacccggtcc aggccgggcg gcgagtcggg
     2161 ccagccccac tcgaagcgcg caccggcggc cgtcaatcca ctctgagcca tagccctggc
     2221 tatcgcccga ttgagggttt gaagatcagc gggcggcgcg gggaggccgg cggccctggc
     2281 cgcgaacacg gcatacatcg attcgcggag gatccgagcc ctcgtcaggg cctcgcctgc
     2341 cgcacgggct ccctggtggg ccgcattgac cagggtggcc gcctctcctg gcgtgaggat
     2401 ggtggcctgt cgagcccacg cgagaagatc ctcataagct ccgatcagat cgagtcgatc
     2461 gctggacggg cgaaaccgga ccgtattcgc gaagtcaaga cagagtcggc cggcgttgaa
     2521 tgcgaacggc tcttccgggg gcgcgctgag ctcggccatg ggccgc
//
LOCUS       DATMCV010000261         9691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_415947, whole genome shotgun sequence.
ACCESSION   DATMCV010000261 DATMCV010000000
VERSION     DATMCV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415947"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VNU03_06045"
     CDS             complement(<1..616)
                     /locus_tag="VNU03_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HWP74052.1"
                     /translation="MSVKKNFYSDNVSGAAPEILEALVAANAGDTAPYGADAITEGLP
                     AKFSALFETAVEVYPVGTGTAANGLCASIVSAPYGAIYLSDAGHLHGSECGGPEFWSG
                     GNARTVPVPSVDGKLVAAGLARLADAAVARPATEVPPRAVSITQGTEASTVYTLAEIR
                     AIVEVARERNLWVHMDGARLGNAVARLGCTPAEATWKAGVDVLSF"
     gene            818..1336
                     /locus_tag="VNU03_06050"
     CDS             818..1336
                     /locus_tag="VNU03_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWP74053.1"
                     /translation="MARFAAAGAVFAFLIAGLVTGPGAQGLVTMQKLSAPLANELVGE
                     SVAQCALKGYTVTAVVVDLDGVRQALLRGNGAPIHTLDNAFFKAYSAASLTLARKEDS
                     TKAVAERMSKSPATTVPQTPLPNVTYAVGGVTIMAGSTPIGAIGVSGAPGGQFDEECA
                     RAAIAKIQERMK"
     gene            1347..1910
                     /locus_tag="VNU03_06055"
     CDS             1347..1910
                     /locus_tag="VNU03_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase"
                     /protein_id="HWP74054.1"
                     /translation="MSDAPTFGRYAELPVDRMTPEQREGYRFLVDGPRGRLPGPYKVW
                     VHNPKLVHAAAPLGQHFTPGQSSVSEREREIAVLVMTGRWHSAYPTAAHEKRGREVGL
                     PAAAVEALVAGQPTSFADPREQVVYEVATALAGGRLVPQGLYDRAVGLLGHEGVTDVI
                     VLMGYYTAVSLTMNFYAVPAGSPGLAR"
     gene            complement(1933..2679)
                     /locus_tag="VNU03_06060"
     CDS             complement(1933..2679)
                     /locus_tag="VNU03_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP74055.1"
                     /translation="MPVFTRPDAEIHYEVHGSGFPLLLFAPGGLRSQLEYWRHSPSNP
                     AAAPPWMNPMADLADRFTVIGMDQRNAGQSHGAVTGTHGWHTYAGDHLALMDHLGHRR
                     FHVMGGCIGGTFCLTLCELAPERIAAAVLQNPIGLHENRDTWDEAVAGFAKTMLERDP
                     TLTEDVIRKFGHNMFGGDFVFSVSRDFVRRCPTPLLLQPGSDKPHPAETSAEIARLAP
                     NLEIQKDWRAPAHLAESISRVGDFLGRHTP"
     gene            2811..4433
                     /locus_tag="VNU03_06065"
     CDS             2811..4433
                     /locus_tag="VNU03_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP74056.1"
                     /translation="MEPSLGPKVLVVYVLGLTGSLAEWNELVDRFKADPAVGHPDSIV
                     YQPKKSLLSVGNAQDLANGLRARIDEKWITEGESYTNVILVGHSFGGLLVRKAYLLAC
                     GAARGTHTSRWHPLVSRIVLFAATNRGVDARRTLRMRLLRRLYRAARTFHAIPHLLLS
                     DALWGSAFLTQLRLEWIRHFRDVDNDRLPEVVQLLGTRDHLVTREDSTDLLQFPKVTA
                     FDVADATHDNLHRPDASEDPASRYKLLRHAVFEPLSAPEPELPPRSKNPVVFVLHGIR
                     ASNSGWPDQLRELLLNANTDAEVILPSYNYFSALNFCLPWIRRRYIEWFQDAYSEALA
                     RSSQSDFHFVGHSNGTYLLGASLKALPAMRFQRIALIGSVLPRDYDWSVRVRDGQVRE
                     IRNHRSSVDWPVGLLCSGLRALQMRDIGTGGFDGFEWNTASVSREIYYHRGGHSGPLT
                     PTCLTDIATHILTGQDPPAPYLVPSPPFFFSWASRLLGHWWFTIPLIAGVLAIAWGAT
                     YLVAMPFHLPPQWHWTFFLGLLLVIAIVLATV"
     gene            complement(4441..5880)
                     /locus_tag="VNU03_06070"
     CDS             complement(4441..5880)
                     /locus_tag="VNU03_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWP74057.1"
                     /translation="MVNHRAATTRTGIIHLINPKTDSLTTRPLYLNRALYSPLAGLLA
                     VAAGIPRDRYEVVLTDENIESIDFDLKADLVGLSAMTSYVNRGYEIADAFRARGVPVV
                     MGGVHPSFMPQEALKHCDAVVIGEAELVLDKLLDDLEQGTMRGTYRADRLHPMEGLSM
                     PRYDLLKKNRYVNRTFVQTSRGCHQGCTFCAEPLMNGLKFRYRPVDEVIREIENCGSR
                     MISINDADFFGTPERPKEVMRALRGRGVTWQAGVTSKLAQDDRMLELAAASGCTLLSI
                     GFESISRSTLTSVHKHVNRPETFAALVEKVHSYGIMVFGLFMFGFDGDDPSVFRETAR
                     FNIEADYDACAYSVLTPYPGTLTWYELKKANRIVSFDWVKYDQANVVYRPAQMTGDEL
                     RLGQNTAYETFYAPSSMARRFPVRGGRRRLQWTIYNLFMKKGAATDRKDAVAEPTAVP
                     DVVPMPPILPLKREWREAVLEGTGGARPS"
     gene            complement(5945..6658)
                     /locus_tag="VNU03_06075"
     CDS             complement(5945..6658)
                     /locus_tag="VNU03_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74058.1"
                     /translation="MSALLEVEGLHAHYDKSHILRGVHLTIGKGEIVSLLGRNGSGRS
                     TTLKAIMGLVPPSAGSVRLDGVDLAGVRAFRVARAGLAYVPEEREIFANLTVDENMRI
                     GMQTARRGAPQWTPEQMYGYFPQIKERRDTQAGMLSGGEQQMLTICRSLLGNPRVILI
                     DEPTEGLAPRVVEVVTEVVRDICRQGVAVLLVEQKLTIALRVAERVYVMGHGQIVFEG
                     TPAALRGAPDVRRDWLEVA"
     gene            complement(6655..7422)
                     /locus_tag="VNU03_06080"
     CDS             complement(6655..7422)
                     /locus_tag="VNU03_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74059.1"
                     /translation="MSAESRGGPVLVLRDLHKSFGATAIIRGVNLEVTRGQRHGLIGP
                     NGAGKSTLFNLISGHYVPSAGEILLNGQPIAGLTPHAINRRGLSRSFQITNLFPRLSV
                     AENLRISVMGRHHHRFTLFRPVTRLTRVAAEVDEYLEKLRLTRRRDDPAGALAYSEQR
                     TLEIGMALATDPEILLLDEPTGGMSREESAYTVALIRTVTEGKTLLVVEHDMNVVFTL
                     CDQLSVLVYGEVIASGRPEAVRANREVQQAYLGEEVA"
     gene            complement(7419..8672)
                     /locus_tag="VNU03_06085"
     CDS             complement(7419..8672)
                     /locus_tag="VNU03_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74060.1"
                     /translation="MSRAGRLGLLALVAILLVLPLPLTPNLVNAAIKMMIAGLFALSF
                     SLAMGQAGMLSFGHSAYYGLGAFATLHLMKAVEGHLVAWPTPLLPLAGAAVGLFFGLV
                     FGWLATQRTGVYFAMVTLALAELLFTLAPTWTSVFGGESGISTMRMNSWAINFATDTG
                     VYYLTLAWVAVSTWCLWAFTRTPMGRLALAVRENEHRVRFLGFNTHGARTVIFAISTM
                     FTGVAGALLAIANEATNYTIFSAQASANVVLQTFIGGAGTFFGPALGAAVMTFFARVT
                     SDLTRSWLLYQGLVFVIVMLFVPDGIGGLISLHARRLRATAVRHLTLPYLLCLVVGVL
                     LMVGVVFTVESVHVVLSDAYGVLRRANGGAMVPYSLFGRQFDPSSPWTWAIPVVSIAG
                     GAALLPLARRLTRRGWSQALLEETA"
     gene            complement(8669..9610)
                     /locus_tag="VNU03_06090"
     CDS             complement(8669..9610)
                     /locus_tag="VNU03_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74061.1"
                     /translation="MEFLVVSVLNGVVHGLLLFMVSAGLTLIFGMMGVLNFAHAWFYM
                     LGAYVGFTLSRTLGFWAGLIAAPIIAGAGGVLVERYMLRRVHKYGHAHELLLTFGLAF
                     IIEEVIKLFYGDFPVNYAMPPYMKFAAFTIYDTNYPFYRLFMGIVALAMFAVLYVVLT
                     RTRVGIIVRAATYRPVMVEALGHNLPMVFMGVFAAGAALAGLAGAVAGAFFPTGPNMA
                     VDFGTLVFVVVVVGGLGSLEGALVASLMIGLFISFSVGFSWSLSDVLALVGLGDWARG
                     VGGLMTLQLSTIAASMPFLLMLLVLLIRPAGLFGDRQ"
BASE COUNT         1524 a         3411 c         3358 g         1398 t
ORIGIN      
        1 cgaaggagag cacgtcgacg cccgccttcc acgtcgcctc ggccggcgtg cagcccaggc
       61 gggcgaccgc gttgcccagg cgcgcgccgt ccatatgcac ccacagattg cgctcgcgag
      121 cgacctcgac gatggcgcgg atctcggcca gcgtgtacac cgtgctggcc tcggtcccct
      181 gggtgatgct gaccgcccgc ggcggaacct cggtggccgg acgcgcgacc gccgcgtcgg
      241 ccagccgcgc gagcccggcc gcgaccagct tgccgtccac gctcggcacc ggcacggtgc
      301 gcgcgttgcc ccccgaccag aactccggcc cgccgcactc gctcccgtgg aggtggccgg
      361 cgtcggagag gtagatggcc ccgtaggggg cggagacgat cgacgcgcag agcccgttgg
      421 cggcggtgcc ggtccccacc ggataaacct cgacggcggt ctcgaagagc gccgagaact
      481 tggccggcag gccctcggtg atcgcgtcgg cgccgtaggg cgcggtgtcc cccgcgttgg
      541 ccgccacgag cgcttccagg atctcgggcg ccgcgcccga cacgttgtcg ctgtagaagt
      601 tcttcttcac gctcacgcaa gcctccctct gtcggcgggc tcgggacgga cggcgcgacg
      661 gcccgggcgc cgcggcccgt ctaccatagg cgaggaggcg gcccccagca accgccgatc
      721 cgcgcggcct cgctggagta tgattcgcgc gggtccggca ctctcgccgc cccgacttcc
      781 aggcgctcaa cggagaacaa ccgacaagga ggggatcatg gctcgattcg cggcggccgg
      841 cgcggtgttc gcgttcctca tcgcaggtct cgtcacgggc cccggggccc agggtctggt
      901 caccatgcag aagctgtcgg cgccgctggc caacgagctg gtcggcgaat cggtcgccca
      961 gtgcgccctg aagggttaca ccgtgaccgc ggtcgtcgtc gacctggacg gggtccgcca
     1021 agcgctcctg cggggcaacg gggcgccgat ccacaccctc gacaacgcct tcttcaaggc
     1081 gtactcggcg gcgtcgctga cgctcgcgcg caaggaggac agcaccaagg ccgtcgccga
     1141 gcggatgtcc aagagccccg cgaccacggt gccgcagacg ccgctgccca acgtcaccta
     1201 cgcggtcggc ggggtgacga tcatggcggg cagcacgccc atcggcgcga tcggcgtgag
     1261 cggggcgccg ggcggccagt tcgacgagga gtgcgcccgc gcggccatcg ccaagatcca
     1321 ggagcggatg aagtagggcc ccgcgcatga gcgacgcccc gaccttcgga cgctacgccg
     1381 agctgccggt cgaccggatg accccggagc agcgcgaagg gtatcggttc ctggtcgacg
     1441 ggccgcgcgg ccgcctcccc gggccctaca aggtgtgggt gcacaacccg aagctggtgc
     1501 acgcggcggc gccgctcggc cagcacttca cgcccggcca gtcctcggtg tcggagcgcg
     1561 agcgggagat cgcggtcctc gtcatgaccg gcaggtggca ttccgcctac cccaccgcgg
     1621 cgcacgagaa gcgcgggaga gaggtcggcc tgccggccgc cgcggtggag gcgctcgtgg
     1681 ccgggcagcc gacctcgttc gccgacccgc gcgagcaggt ggtctacgag gtggcgacgg
     1741 ccctcgccgg cggtcgcctc gtgcctcagg ggctctacga ccgcgcggtg gggctcctgg
     1801 gccacgaggg cgtcaccgac gtgatcgtcc tcatgggcta ctacaccgcg gtctcgctga
     1861 ccatgaactt ctacgcggtg ccggcgggga gcccgggcct ggcgcgctga gggcgccggg
     1921 tcagtcgccc ttctacgggg tgtgacgccc gaggaagtcg ccgacccggc tgatcgactc
     1981 ggccaggtgg gccggggccc gccagtcctt ctggatctcg aggttcgggg ccaggcgggc
     2041 gatctccgcg ctggtctcgg cggggtgcgg cttgtcgctg ccgggctgga gcaggagcgg
     2101 ggtggggcag cgccgcacga agtcgcggct caccgagaag acgaagtcgc cgccgaacat
     2161 gttgtggccg aacttccgga tgacgtcctc ggtcaaggtc gggtcgcgct ccagcatggt
     2221 cttggcgaag ccggccaccg cctcgtccca ggtgtcccgg ttctcgtgca ggccgatcgg
     2281 gttctgcagc accgcggcgg cgatgcgctc cggggccagc tcgcacaggg tcaggcagaa
     2341 ggtgccgccg atgcagccgc ccatgacgtg aaagcggcgg tggccgagat gatccatcag
     2401 ggccaggtga tcgcccgcgt aggtgtgcca cccgtgggtc ccggtcaccg caccgtggga
     2461 ttggcccgcg ttgcgctggt ccatgccgat cacggtgaac cggtcggcca ggtcggccat
     2521 ggggttcatc caggggggcg cggcggcggg gttactcggg ctgtggcgcc agtactcgag
     2581 ctgcgagcgc aggccgcccg gcgcgaagag cagcagcgga aagccggagc cgtggacttc
     2641 gtagtggatc tcggcatcgg gtcgcgtgaa taccggcatc gtcatcctcc cgggggtgtg
     2701 gtcaccgcga ttgccgggcc acgataacat cgttgggcgg cgctccgtcg gcccagtaag
     2761 ggcacatcgt cgttgacagc ctccccgtca cgccctacgg tgcggccacc gtggagccca
     2821 gtctcgggcc gaaagtcctc gtggtctacg tcctcggcct caccggttcc ctcgccgagt
     2881 ggaacgagct ggtggaccgg ttcaaggcgg atccggcggt ggggcatccc gactcgatcg
     2941 tctaccagcc gaagaaatcc ctgctgagcg tcggcaacgc ccaggacctc gccaacggcc
     3001 tgcgggctcg catcgacgag aagtggatca ccgaggggga gagctacacc aacgtgatcc
     3061 tggtcggcca cagctttggc ggccttctcg tccggaaggc ctatctcctg gcctgcggag
     3121 ccgcgcgagg cactcacacc tcgcggtggc atcccctcgt cagccgtatc gtcctgttcg
     3181 ccgccaccaa tcgaggcgtc gacgcccggc ggaccctgcg catgcgcctc ctgcgccgtc
     3241 tctaccgcgc cgcccggacg tttcatgcca tccctcacct cctcctgagc gacgcgctgt
     3301 ggggctcggc cttcctcacc cagcttcgcc tggaatggat tcgccacttc cgtgacgtcg
     3361 acaacgaccg gctcccggaa gtcgtccagc tgctcggcac ccgggaccat ctggtcaccc
     3421 gggaagacag cacggacctc ctccagttcc cgaaggtcac cgccttcgac gtcgccgacg
     3481 ccacccacga caacctgcac cggcccgacg cctcggagga cccggcctcc cgctacaagc
     3541 tgctgcgcca cgccgtcttc gagccgctct ccgcgcccga gccggagctg cctccccgct
     3601 ccaagaaccc ggtggtcttc gtgctccacg gcatccgggc gtcgaacagc ggctggccgg
     3661 atcagctgag ggagctgctg ctcaacgcca acaccgacgc cgaggtcatt ctccccagct
     3721 acaactactt ctcggccttg aacttctgcc tcccgtggat ccgccggcga tacatcgagt
     3781 ggttccagga cgcctacagc gaagcgctgg cgcgttcctc ccagagcgac ttccacttcg
     3841 tcgggcacag caacggcacc tacctgctcg gcgcctcgct caaggccctg cccgccatgc
     3901 gcttccagcg aatcgccctg atcggctccg tcctgccccg cgactacgac tggagcgtgc
     3961 gcgtgcgcga cggccaggtc cgggagatcc ggaatcatcg gtcgtcggtc gattggccgg
     4021 tgggactcct gtgcagcggt cttcgcgccc tgcagatgag ggacatcgga accggcggat
     4081 tcgacggctt cgaatggaac accgcctcgg tcagccggga gatctactat catcgaggag
     4141 gacacagcgg cccgctgacg ccgacgtgtc tgaccgatat cgcgacccac atcctcaccg
     4201 gccaggaccc gcccgccccg tacctcgttc cgagcccgcc cttcttcttc tcctgggcct
     4261 ctcgcctgct gggtcactgg tggttcacga ttccgctgat cgccggcgtc ctggccatcg
     4321 cgtggggggc cacctacctg gtcgcgatgc ccttccacct cccaccccag tggcactgga
     4381 cgttcttcct cggtctcctt ctcgtcatcg ccatcgtcct cgcgaccgtg tagccgcgat
     4441 tcagctcggg cgcgccccgc cggtcccttc cagcaccgcc tcgcgccatt cccgcttgag
     4501 cggcaggatc ggcggcatcg gcaccacgtc cggcaccgcg gtcggctcgg cgaccgcgtc
     4561 cttgcgatcc gtcgcggccc ccttcttcat gaacaggttg tagatggtcc actggagccg
     4621 ccggcggccg ccgcggaccg gaaagcgccg cgccatcgac gacggggcgt agaacgtctc
     4681 gtaggcggtg ttctggccca gccgcagctc gtcgccggtc atctgggccg gccggtacac
     4741 cacgttggcc tgatcgtact tcacccagtc gaaggagacg atgcggttgg ccttcttgag
     4801 ctcgtaccag gtgagcgtgc ccgggtaggg cgtgaggacc gagtacgcgc acgcgtcgta
     4861 gtccgcctcg atgttgaacc gcgccgtctc gcggaacacg gacggatcgt cgccgtcgaa
     4921 gccgaacatg aacaggccga acaccatgat gccgtaggag tgcaccttct ccacgagcgc
     4981 ggcgaaggtc tcgggacggt tcacgtgctt gtggacgctg gtgagggtgg accgcgagat
     5041 cgactcgaag ccgatgctca gcagcgtaca cccgctcgcg gcggcgagct cgagcatccg
     5101 atcgtcctgc gcgagcttcg aggtgacccc ggcctgccag gtcaccccgc ggccccggag
     5161 cgcgcgcatg acctccttcg ggcgctcggg ggtgccgaag aagtcggcgt cgttgatcga
     5221 gatcatccgc gagccgcagt tctcgatctc ccggatcacc tcgtcgaccg gccggtagcg
     5281 gaacttgagg ccgttcatca ggggctcggc gcagaacgta cagccctggt ggcagccgcg
     5341 cgaggtctgc acgaaggtgc ggttcacgta gcggttcttc ttgagcaggt cgtaccgcgg
     5401 catggacagg ccttccatcg ggtgcagccg gtccgcccgg taggtgccgc gcatcgtgcc
     5461 ctgctcgaga tcgtccagga gcttgtcgag caccagctcc gcctcgccga tcaccaccgc
     5521 gtcgcagtgc ttgagcgcct cctggggcat gaagctcggg tgcacgccgc ccatcacgac
     5581 cggcactccc cgcgcgcgga aggcgtcggc gatctcgtac ccgcggttca cgtagctcgt
     5641 catcgcggag agcccgacca gatccgcctt caggtcgaag tcgatcgact cgatgttctc
     5701 gtcggtcagc acgacctcgt agcggtcgcg cgggatgccg gccgcgaccg cgagcagacc
     5761 cgcgagcggg gagtagaggg cccggttcag atagagcggg cgggtggtga gcgaatccgt
     5821 cttcggattg atgaggtgga tgattccggt ccgcgtggtg gccgcccgat gattgaccat
     5881 ggccgggtcc tcctgtcggg aatggtaggt ccgccgcggc gggcggtcaa gatatccgag
     5941 cgcctcaggc gacctccagc cagtcgcgcc ggacgtccgg ggcgccgcgc agcgcggccg
     6001 gggtgccctc gaagacgatc tggccgtggc ccatcacgta cacgcgctcc gcgacccgga
     6061 gcgcgatggt gagcttctgc tcgaccagca gcaccgcgac gccctgccgg cagatgtcgc
     6121 ggacgacctc ggtgaccacc tcgacgaccc tcggggccag cccctcggtc ggctcgtcga
     6181 tcaggatcac gcgcgggttg ccgagcagcg agcggcagat ggtcagcatc tgctgctccc
     6241 cgcccgagag catgccggcc tgggtatcgc ggcgctcctt gatctgggga aagtagccgt
     6301 acatctgctc gggggtccac tggggggcgc cccgccgggc ggtctgcatg ccgatccgca
     6361 tgttctcgtc gacggtcagg ttggcgaaga tctcgcgctc ctcgggcacg taggccagcc
     6421 ccgcgcgggc gacgcggaac gcccgcaccc ctgcgaggtc gacgccgtcg agccgcaccg
     6481 agccggcgct cggcgggacc agccccatga tcgccttcag ggtcgtggag cggccggagc
     6541 cgttgcgccc gagcaggctc acgatctcgc ccttcccgat ggtgaggtgc accccgcgca
     6601 ggatgtggct cttgtcgtag tgggcgtgca ggccctcgac ctcgaggagc gcgctcacgc
     6661 gacctcctcg cccaggtagg cctgctggac ctcgcggttg gcccgcaccg cttcgggccg
     6721 gccgctcgcg atgacctcgc cgtagacgag caccgagagc tggtcgcaga gggtgaacac
     6781 cacgttcatg tcgtgctcga ccaccagcag tgtcttgccc tcggtgaccg tccggatgag
     6841 cgccaccgtg taggcggact cctcgcgcga catgccgccg gtcggctcgt ccaggaggag
     6901 gatctcgggg tcggtggcga gcgccatgcc gatctcgagg gtgcgctgct cggagtaggc
     6961 gagggcgccg gcgggatcgt cgcggcgacg ggtcagacgc agcttctcga ggtactcgtc
     7021 gacctcggcg gccacccgcg tgagccgggt caccggacgg aagagggtga agcgatggtg
     7081 atgccggccc atcaccgaga tgcgcaggtt ctcggccacg ctcaggcgcg ggaagaggtt
     7141 ggtgatctgg aacgagcgcg acaggccgcg gcgattgatc gcgtgcggcg tcaaccccgc
     7201 gatcggctgg ccgttgagca ggatctcccc cgcgctcggc acgtagtggc cggagatcag
     7261 gttgaagagg gtcgacttgc ccgccccgtt ggggccgatc agcccgtggc gctgaccccg
     7321 cgtcacctcc aggttgaccc cgcggatgat cgcggtcgcg ccgaacgact tgtgcagatc
     7381 gcgcagcacc aggaccggcc cgccccggct ctcggcgctc acgcggtttc ctcgaggagc
     7441 gcctggctcc acccccggcg ggtgaggcgc cgggcgagcg gcagcagcgc ggcgccgccc
     7501 gcgatcgaga ccaccgggat cgcccaggtc cacggagacg aagggtcgaa ctgacgaccg
     7561 aacagcgagt acgggaccat ggccccgccg ttggcccggc gcagcacccc gtaggcgtcc
     7621 gacagcacca cgtgcacgct ctcgacggtg aagacgacgc cgaccatgag cagcaccccg
     7681 accacgaggc acaggagata cggcagggtc aggtggcgca ccgcggtggc gcgaagccgg
     7741 cgggcgtgca gggagatcag gccgccgatg ccgtccggca cgaacagcat cacgatcacg
     7801 aagacgagcc cctggtagag cagccacgag cgcgtgagat cggaggtcac ccgcgcgaag
     7861 aaggtcatga ccgcggcccc gagggccggg ccgaagaacg tgcccgcccc gccgatgaag
     7921 gtctgcagca ccacgttggc ggaggcctgc gcgctgaaga tcgtgtagtt ggtggcctcg
     7981 ttggcgatgg cgagcagggc ccccgccacg ccggtgaaca tggtcgagat ggcgaagatc
     8041 accgtccggg cgccgtgggt gttgaagccg aggaagcgga cccggtgctc gttctcgcgg
     8101 accgcgagcg cgagccggcc catcggggtc cgggtgaagg cccacaggca ccaggtcgag
     8161 acggcgaccc aggcgagcgt caggtagtag accccggtgt cggtcgcgaa gttgatcgcc
     8221 caggagttca tccgcatggt ggagatgccg gactcgcccc cgaacaccga cgtccaggtg
     8281 ggcgccagcg tgaacagcag ctccgcgagc gcgagcgtca ccatcgcgaa gtacaccccg
     8341 gtgcgctggg tggcgagcca gccgaacacg aggccgaaga agaggccgac cgccgcgccg
     8401 gccagcggca gcagcggggt cggccacgcc accaggtgcc cctccaccgc cttcatcagg
     8461 tggagcgtgg cgaaggcccc gaggccgtag tacgcggagt gaccgaagga cagcattccg
     8521 gcctgcccca tcgcgaggct gaacgagagc gcgaacagcc ccgcgatcat catcttgatc
     8581 gcggcgttga ccaggttggg agtgagcggc agcggcagca cgagcaggat cgcgacgagc
     8641 gcgaggagtc ccagccggcc cgcccggctc actggcggtc gccgaagagc ccggcgggac
     8701 ggatcagcag caccagcagc atgagcagga agggcatgct ggccgcgatg gtcgagagct
     8761 gcagcgtcat cagcccgccc accccgcgcg cccagtctcc gaggccgacc agggccagca
     8821 cgtcggagag cgaccagctg aagccgaccg agaacgagat gaagagcccg atcatcagcg
     8881 aggcgaccag cgcgccctcg agcgagccga gcccgccgac caccaccacc acgaagacca
     8941 gggtgccgaa gtcgaccgcc atgttcggcc cggtcggaaa gaacgcgccc gccaccgccc
     9001 cggcgagccc ggccagcgcc gcgccggccg cgaagacccc catgaagacc atcggcagat
     9061 tgtggccgag ggcctcgacc atcaccggcc ggtaggtggc ggcccgcacg atgatgccga
     9121 cccgggtgcg ggtcagcacc acgtagagca ccgcgaacat cgcgagggcc acgatgccca
     9181 tgaacaggcg gtagaaggga tagttcgtgt cgtagatggt gaacgcggcg aacttcatgt
     9241 agggcggcat cgcgtagttc accgggaagt cgccgtagaa cagcttgatg acttcctcga
     9301 tgatgaaggc gaggccgaag gtcagcagca gctcgtgggc gtggccgtac ttgtgcaccc
     9361 ggcgcagcat gtagcgctcg acgagcaccc cgccggcccc cgcgatgatc ggcgccgcga
     9421 tcagcccggc ccagaagccg agggtgcgcg agagcgtgaa gcccacgtag gcgcccagca
     9481 tgtagaacca ggcgtgggcg aagttgagca cgcccatcat cccgaagatg agggtgaggc
     9541 cggcggacac catgaacagc aggagcccgt gcaccacccc gttcaacacc gagacgacga
     9601 ggaactccac gccgcgcgct ccgctccggg gcgcccgcta cctaggagcc cggccgctcc
     9661 atcttgcagc tcgcctgcac cgggctcgcg g
//
LOCUS       DATMCV010000262         4954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_480518, whole genome shotgun sequence.
ACCESSION   DATMCV010000262 DATMCV010000000
VERSION     DATMCV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480518"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VNU03_06095"
     CDS             complement(<1..330)
                     /locus_tag="VNU03_06095"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74062.1"
                     /translation="MTRYLAVRLYSMGLTLIGLTLLIFLMLRLIPGTVVEQMIGADAV
                     ASPAMVAELKRFFGLDQPWYVQYVKWLGRLSHGDLGTSWRTGKPVILLILERLPVTLE
                     LTMLSVGF"
     gene            complement(476..2044)
                     /locus_tag="VNU03_06100"
     CDS             complement(476..2044)
                     /locus_tag="VNU03_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74063.1"
                     /translation="MTIDPGHIDASRLSRRQLLQAGGLALGALAVARPAAAQSPRTGG
                     SLISAQATEATGLDPQLVPALSRSRRSPLTYNQLVRFDDQMNPTTELAESWETSKDGL
                     SWTFKLRQGVKFHDGQEMTSADVKFTFDRLFEKSPGKSDFIAVDKVEPAGKYAVKFIT
                     KEPFAGMLAALGGFWGFIISEAGVKAAGGDLNKTAMGTGPYMFVDWKVEQQLVLKKHP
                     GYFKKGLPHVDELTVRTIPDEANIVAALRTGQIHHAFIEDNKNYNLLKEEKTLTGYRS
                     SRLGYDYLNMSANRGPLKDVRVRQAISWAVDRSQVLRVAAAGFGRLTAPATAPMKQWQ
                     LPEEAWMKYYKPDVEKAKKLMAEAGQSAGFTVKCMVIPTFPTMVAGAPVISAQLKRIG
                     ITMEIEQVEYAIWIKRWLAHDFDMTMNSTPGYADPDTAFFRALHSTKGQNWNSWSVPE
                     LDALLEDGRRTMDQKKRKEIYDRVQIMILEQVPHLWLFSADTIDFTQASVKGFKQHPT
                     ALLYGLEGAWLDKA"
     gene            complement(2085..2951)
                     /locus_tag="VNU03_06105"
     CDS             complement(2085..2951)
                     /locus_tag="VNU03_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74064.1"
                     /translation="MLGSTGVVPHRLRWSRQLRRVTHSVVVVAGALCVALIVAVAVFA
                     GSIAPQDPDEQNFDLIEARPGAKALFGTDRFGRDVLSRVIHGSRISLYVAVTSITVAM
                     AAGGVLGLTGGYLGGGWDNVINRVLDVFFSIPGLLLSVGIAAMRGPGVNSAVIAIAIV
                     YVPLFARVMRGPVLAEREKEYVEAIRALGATRWVVALKHVLPNVLSPFVVQGTVALSQ
                     AILIEASLSYLGLSAQPPTPSWGNMLNEGRTYLETAPWISVFPGIAIMVTVLAFNLLG
                     DGLRDVLDPSGH"
     gene            3073..3876
                     /locus_tag="VNU03_06110"
     CDS             3073..3876
                     /locus_tag="VNU03_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWP74065.1"
                     /translation="MRVRLGVSVMCAVVATLAPAARAQVPDTVLLEDLTWTEVRDAVR
                     SGKTTIIVPSGGTEQNGPHMALGKHNARVRILSERIARSLGDALVAPVIAYVPEGGIA
                     PPSGHMRFAGTITTPEDAFEKVLESAARSFKLHGFRHIVLLGDHGSTQAGQKAVAARL
                     NREWAATPVRVLAIDEYYRAAEVEAAQLLKARGYRDAELGRHAGLTDTALTLATEPRM
                     VRADRLRPARDGDGVDGDPSRATAELGRAGVELIVTRTVEAIRKSIPTR"
     gene            3996..>4954
                     /locus_tag="VNU03_06115"
     CDS             3996..>4954
                     /locus_tag="VNU03_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWP74066.1"
                     /translation="MPPVVNPTNLYSEIRPDNVSPTVSGALSRIYVPNRQGNDVWVID
                     PATMRVVDKFPVGVNPQHVVPSWDLKTLWVTNNAEGRTDGTLTPIDPTTGKPGKSIAV
                     DDPYNMYFSPDGRSAIVVAEALKRLDLRDPQTMALQGSIPVPQCKGINHADFSIDGRI
                     AIFTCEFQGGLARIDMVGRKVLGYLKLSRGGMPQDIRVSPDGKIFYVADMKAGGVFVI
                     DGDAFKEIGFLKTGIGTHGLYPSRDGSKLYVANRGSAHVFGPPRGKGTVSVIDFTSRL
                     IVATWPIPGGGSPDMGNVSIDGKVLWLSGRFDNVVYAIDTATG"
BASE COUNT          759 a         1645 c         1783 g          767 t
ORIGIN      
        1 gaatcccacc gacagcatgg tcagctcgag cgtcacgggc agccgctcga ggatgagaag
       61 gatcacgggc ttgccggtac gccacgacgt gcccaggtcc ccgtgagaga gccggccgag
      121 ccacttgacg tactggacgt accacggctg gtcgagcccg aagaagcgct tgagctcggc
      181 caccatggcc gggctggcga cggcgtcggc gccgatcatc tgctcgacca cggtgcccgg
      241 gatcaggcgg agcatgagaa agatcaggag cgtgaggcct atcagcgtca ggcccatcga
      301 gtacaggcgg acggccagat agcgggtcat ggtggggcga ccggctggcg gacagccgtt
      361 tgcggcagag cattgttctg attggggggg agcggcgccg ctcctccccc aagccccccc
      421 accgccctat gcatcgcgtt ctatcgaggc gttgcgttgt ggtttcgggt ttgccctagg
      481 ctttgtccag ccaggcgccc tcgaggccgt agagcagggc ggtggggtgc tgcttgaagc
      541 ccttcacgct ggcctgggtg aagtcgatgg tgtcggcgga gaagagccac agatgcggca
      601 cctgctccag gatcatgatc tgcacccggt cgtagatctc cttgcgcttc ttctgatcca
      661 tggtgcggcg gccgtcctcg agcagcgcat cgagctcggg cacgctccag ctgttccagt
      721 tctggccctt ggtggagtgg agcgcccgga agaacgcggt gtccggatcg gcgtagccgg
      781 gggtggagtt catggtcatg tcgaagtcgt gggcgagcca gcgcttgatc cagatcgcgt
      841 actccacctg ctcgatctcc atggtgatgc cgatgcgctt gagctgggcc gagatgaccg
      901 gcgcccccgc taccatggtg gggaaggtgg ggatgaccat gcacttcacg gtgaagcccg
      961 cgctctgccc ggcctccgcc atgagcttct tggccttctc cacgtccggc ttgtagtact
     1021 tcatccacgc ctcttccggc agctgccact gcttcatcgg cgcggtggcc ggggcggtga
     1081 ggcggccgaa gccggccgcg gccacccgca gcacctggct gcggtcgacc gcccagctga
     1141 tggcctggcg cacccgcacg tccttcagcg ggccgcggtt ggcgctcatg ttgaggtagt
     1201 cgtagcccag gcgcgagctg cggtagccgg tgagggtctt ctcctccttc aggaggttgt
     1261 agttcttgtt gtcctcgatg aaggcgtggt ggatctggcc ggtgcgcagc gcggccacga
     1321 tgttggcctc gtccgggatg gtgcggacgg tcagctcgtc gacatggggc agccccttct
     1381 tgaagtagcc ggggtgcttc ttcagcacga gctgctgctc caccttccag tccacgaaca
     1441 tgtagggccc ggtgcccatc gcggtcttgt tgaggtctcc ccccgcggcc ttcaccccgg
     1501 cctcgctgat gatgaagccc cagaagccgc cgagggcggc gagcattccc gcgaagggct
     1561 ccttggtgat gaacttcacc gcgtacttgc ccgccggctc caccttgtcc accgcgatga
     1621 agtcggactt gccggggctc ttctcgaaga ggcggtcgaa ggtgaacttg acgtcggcgc
     1681 tggtcatctc ctggccgtcg tggaacttca ccccctgccg cagcttgaag gtccagctga
     1741 ggccgtcctt gctcgtctcc cacgactcgg cgagctcggt ggtcgggttc atctggtcgt
     1801 cgaagcgcac gagctggttg taggtgaggg gggagcgccg gctgcgcgag agcgcgggga
     1861 cgagctgggg atcgagcccg gtggcctcgg tggcctgcgc ggagatgagc gagccgccgg
     1921 tccgggggct ctgggccgcg gcggggcggg cgaccgcgag ggcgccaagg gcgaggccgc
     1981 ccgcctgcag gagctggcgg cggctgagac ggcttgcgtc gatgtggccg ggatcgatcg
     2041 tcatggggcg ttcctcctgt gaggggtcgc gggtgagggc cgggtcagtg gccactgggg
     2101 tcgaggacgt cgcgcaggcc gtcgcccagc aggttgaagg cgaggacggt gaccatgatc
     2161 gcgatgccgg ggaagaccga gatccacggc gcggtttcga gataggtgcg gccttcgttg
     2221 agcatgttgc cccaggacgg ggtgggcggc tgggccgaga ggcccaggta gctgagcgag
     2281 gcctcgatga ggatcgcctg cgacagggcc accgtgccct ggaccacgaa cggcgagagg
     2341 acgttgggca gcacgtgctt gagggcgacc acccagcggg tggccccgag ggcgcggatg
     2401 gcctccacgt actccttctc gcgctcggcc agcaccggcc cgcgcatgac gcgggcgaag
     2461 agcggcacgt agacgatcgc gatggcgatg accgcgctgt tgaccccggg cccgcgcatc
     2521 gcggcgatgc ccaccgagag cagcaacccc gggatgctga agaacacgtc cagcacgcgg
     2581 ttgatcacgt tgtcccagcc gccgcccagg tacccgccgg tgaggccgag gacgccgccc
     2641 gcggccatcg ccaccgtgat cgaggtgacc gcgacgtaga gcgagatgcg ggagccgtgg
     2701 atcacccggc tcagcacgtc gcggccgaag cggtcggtgc cgaagagggc cttcgcgccg
     2761 ggccgggcct cgatcaggtc gaagttctgc tcgtcgggat cctggggcgc gatggagccc
     2821 gcgaagaccg cgaccgccac gatgagagcc acgcacaggg cgcccgcgac gaccacgaca
     2881 ctgtgggtga cgcgacggag ctgacggctc catcgaagtc ggtgcggcac gacgcccgtc
     2941 gagccgagca ccccttcggt cctgacttcc acgggggaac actaggctct gcgcccgcac
     3001 ctgtcaagcg cgcggcggcc ggggcgggga aagatttcgg agggttaatc gttacagctt
     3061 atgatcgggt tgatgcgcgt ccggctcggg gtgagcgtga tgtgcgcggt ggtcgcgacg
     3121 ctggcgccgg cggcgcgggc ccaggtcccg gacaccgtgc tcctggagga cctgacctgg
     3181 accgaggtcc gcgacgcggt ccggtccggc aagaccacga tcatcgtccc gtcggggggc
     3241 accgagcaga acggccccca catggccctg ggcaagcaca acgcgcgggt gcggatcctc
     3301 tccgagcgga tcgcccgctc gctcggggac gccctcgtgg ccccggtgat cgcctacgtc
     3361 ccggaaggcg ggatcgcgcc gccgtccggg cacatgcgct tcgcgggcac catcaccacg
     3421 ccggaggacg ccttcgagaa ggtgctcgag tccgccgcgc ggagcttcaa gctgcacggc
     3481 ttccgtcaca tcgtgctgct cggcgatcac gggtcgacgc aggccggcca gaaggcggtg
     3541 gccgcccggc tgaaccgcga gtgggccgcc accccggtgc gggtcctggc gatcgacgag
     3601 tactaccgcg ccgcggaggt ggaggcggcg cagctcctga aggcccgcgg gtaccgcgac
     3661 gccgagctgg gacgccacgc ggggctcacc gacaccgcgc tgaccctcgc caccgagccc
     3721 cggatggtgc gggcggaccg cctgcggccc gcccgcgacg gggacggggt cgacggcgac
     3781 ccgagccgcg ccaccgccga gctgggacgg gccggggtcg agctgatcgt gacccggacc
     3841 gtggaggcca ttcgcaaatc catcccgacg cgctgaagga gacgatcggc atgacgactc
     3901 gactgcgctt catctcgctc gcggccgcgg tgtgcctctg gggcggcgtg gccgcggccc
     3961 agaccggccc gcgcccggtg gagacgatcc ccgggatgcc gccggtggtg aacccgacca
     4021 acctctacag cgagatccgc ccggacaacg tgagcccgac ggtgagcggc gcgctgtcgc
     4081 gcatctacgt gcccaatcgc cagggcaacg acgtctgggt gatcgatccc gccaccatga
     4141 gggtcgtcga caagttcccg gtgggggtga acccgcagca cgtggtgccc tcgtgggacc
     4201 tcaagaccct gtgggtgacc aacaacgcgg agggccggac cgacggcacc ctgaccccga
     4261 tcgatccgac caccggcaag ccgggcaagt cgatcgcggt cgacgatccc tacaacatgt
     4321 acttctcgcc ggacgggcgc tccgccatcg tggtggcgga ggcgctcaag cggctcgacc
     4381 tgcgggatcc gcagacgatg gccctgcagg gctcgatccc ggtgccgcag tgcaagggga
     4441 tcaaccacgc cgacttctcc atcgacgggc gcatcgccat cttcacctgc gagttccagg
     4501 gcgggctggc ccggatcgac atggtcggcc gcaaggtgct cggctacctc aagctctccc
     4561 gcgggggcat gccgcaggac atccgcgtct cgcccgacgg caagatcttc tacgtggccg
     4621 acatgaaggc gggcggggtg ttcgtgatcg acggcgacgc cttcaaggag atcggcttcc
     4681 tcaagaccgg catcgggacc cacgggctct acccgagccg cgacggctcc aagctctacg
     4741 tggccaaccg cggctccgcg cacgtgttcg ggcccccgcg gggcaagggg accgtctcgg
     4801 tcatcgactt cacgagccgg ctgatcgtcg ccacctggcc gatcccgggc ggcggcagcc
     4861 ccgacatggg taacgtgagc atcgacggca aggtgctgtg gctctccggc cgcttcgaca
     4921 atgtcgttta cgcgatcgac accgcgaccg gcga
//
LOCUS       DATMCV010000263         3755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_614949, whole genome shotgun sequence.
ACCESSION   DATMCV010000263 DATMCV010000000
VERSION     DATMCV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614949"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /gene="groL"
                     /locus_tag="VNU03_06120"
     CDS             complement(<1..1388)
                     /gene="groL"
                     /locus_tag="VNU03_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554876.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWP74067.1"
                     /translation="MSKQLQFKEEARAALLRGVNVLSHAVKVTLGPKGRNVVIDKKFG
                     SPTITKDGVTVAKEIELKDHFENMGAQMLKEVASKTSDIAGDGTTTATVLAQAIFRAG
                     LKNVTAGANPTALQRGIERAVEKVVQELQKMSKSTKDKKEIAQVATIAANNDATVGSL
                     IAEAMEKVGKDGVVTVEEAKGMDTTLEVVEGMQFDRGYLSPYFVTDAERMEAVLEDCL
                     ILISEKKLSVMKDMLPLLEQVAQSGKPLLIVAEDVEGEGLATLVVNKLRGTLSACAVK
                     APGFGDRRKAMLEDIALLTAGKAITEDLGIKLENLKLEDLGRAKKVVVTKDNTTLIDG
                     AGKSASIEGRIKQIRAQIEETTSDYDREKLQERLAKLAGGVAVIKVGAATETAMKEKK
                     ARVEDALNATRAAVEEGIVPGGGVALLRASVALDSLKLSGDEATGVDIVRRALEEPLR
                     QIVHNAGLEGSIV"
     gene            complement(1441..1752)
                     /locus_tag="VNU03_06125"
     CDS             complement(1441..1752)
                     /locus_tag="VNU03_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWP74068.1"
                     /translation="MAQAKHTTTLHPLHDRVLIKRIEDAPARRGAIIIPDSAKEKPQE
                     GKVIAVGKGRVSEDGKKIPLDVKAGDRVLFGKYSGNDITLDGQDLLIMKEEDVLGVIA
                     S"
     gene            1855..2202
                     /locus_tag="VNU03_06130"
     CDS             1855..2202
                     /locus_tag="VNU03_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74069.1"
                     /translation="MTSPATDAHAFSEVFGQREGPYHLHDAGGRPLIRAESGAHVLGA
                     LLRKRTRLVFLDDAGCPELSALRIAYPTREWCPQPPEPTACECEQSGCHGRLVITLHD
                     AHAGPGWMLTFRG"
     gene            2219..2611
                     /locus_tag="VNU03_06135"
     CDS             2219..2611
                     /locus_tag="VNU03_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74070.1"
                     /translation="MEPSKVKASLGQRWSAARPTKTSVFWFGVGAAVVTMIVGFTWGG
                     WVTGGTARRMAEASGEEAVAKRMAPMCVVRFKADPDRSEKLKQLRAVSAWEQGEFVKK
                     QGWATMPGDKEPESRTADECVKLLLLVS"
     gene            2621..3091
                     /locus_tag="VNU03_06140"
     CDS             2621..3091
                     /locus_tag="VNU03_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74071.1"
                     /translation="MTLTRAIDDLARAGFGEHFGVDGDDLRSFTSGRRYRAAEVVIRE
                     FHRFEGVSDPDDMCIVYAIEAQGGARGTLADAFGVYSDPVVSAFFEKVPIRGARRFGG
                     GLDERPWEFGSPGEIRQRKRIPEPARFGAYRHGVPLPVPSDPWQDEGGESGPGQ"
     gene            3179..>3755
                     /locus_tag="VNU03_06145"
     CDS             3179..>3755
                     /locus_tag="VNU03_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP74072.1"
                     /translation="MSGDWVVWQVVVGAVFILSLLLLAIGLMFERAWRRRAEVLLGER
                     LQFETLLSEQVATFSRISAADVDREIQRALRRIADLLAVDWGNLTEYSDGSRTARVTH
                     WWVADGVGPQPEAIGFEEIPWVVTRLRRGELVRFSKIDELPEGDATVDRETYRRLGVK
                     SQVAVPLTVEGTVVGALAFSTVGTERAWRDEF"
BASE COUNT          510 a         1206 c         1367 g          672 t
ORIGIN      
        1 acgatggagc cctccagccc tgcattgtgc acgatctgac gcagcggctc ctcgagcgcc
       61 cgccgcacga tgtccacgcc ggtcgcctcg tcgccggaca gcttgagcga gtcgagggcc
      121 accgaggccc gcagcagcgc gaccccgccg cccggcacga tgccttcctc caccgcggcc
      181 cgcgtcgcgt tgagcgcatc ctccacgcga gccttcttct ccttcatggc ggtctcggtc
      241 gcggcgccga ccttgatgac cgcgacgccg cccgccagct tggcgagccg ctcctgcagc
      301 ttctcccggt cgtagtccga ggtggtctcc tcgatctggg cccggatctg cttgatgcgc
      361 ccctcgatgc tcgcggactt gcccgccccg tcgatgaggg tggtgttgtc cttggtgacg
      421 accaccttct tggcccggcc caggtcttcg agcttcaggt tctcgagctt gatccccagg
      481 tcttccgtga tggccttgcc cgcggtgagc agggcgatgt cttccagcat ggccttgcgg
      541 cggtcgccga agcccggcgc cttgaccgcg cacgcggaga gggtcccgcg cagcttgttg
      601 accacgaggg tggccagccc ctcgccttcc acgtcctccg cgacgatcag gaggggcttg
      661 cccgactggg cgacctgctc cagcaggggc agcatgtcct tcatgacgct gagcttcttc
      721 tcggagatca ggatcaggca gtcctcgagg accgcttcca tgcgctcggc gtctgtcacg
      781 aaataggggg acaggtagcc gcggtcgaac tgcatgccct cgacgacctc gagggtggtg
      841 tccatcccct tggcctcctc gacggtgacc accccgtcct tgccgacctt ctccatggcc
      901 tcggcgatga ggctgccgac ggtcgcgtcg ttgttggccg cgatggtggc gacctgcgcg
      961 atctccttct tgtccttggt ggacttcgac atcttctgga gctcctggac caccttctcg
     1021 accgcccgct cgatgccccg ctgcagcgcg gtggggttcg cgcccgcggt cacgttcttg
     1081 aggccggcgc ggaagatggc ctgggccagg acggtggcgg tggtggtgcc gtcgcccgcg
     1141 atgtcggagg tcttggaggc gacctccttg agcatctggg cgcccatgtt ctcgaagtgg
     1201 tctttcagct cgatttcctt ggcgacggtc accccgtcct tcgtgatggt ggggctgccg
     1261 aacttcttgt cgatgacgac gttgcggccc ttggggccca gggtcacctt gaccgcgtgg
     1321 gacaggacgt tgaccccgcg caggagggcc gcgcgggctt cctctttgaa ctggagctgc
     1381 tttgacatgg atctgttcct ccggagaatg tggactggac gttaactgcg actgcgaatg
     1441 tcaggaagcg atgacgccga ggacgtcctc ctctttcatg atgaggaggt cctggccgtc
     1501 gagggtgatg tcgttgccgg agtactttcc gaagagcacg cgatcgcccg ccttgacgtc
     1561 gagcgggatc ttcttgccgt cttccgatac ccgtcccttg ccgaccgcga tgaccttgcc
     1621 ctcctgcggc ttctccttgg cgctgtccgg aatgatgatg gcgccccgcc gagccggcgc
     1681 gtcctcgatc cgcttgatga ggacccggtc gtgcagggga tgtagcgtcg tggtgtgttt
     1741 tgcctgagcc atgatgtcgg tccctcccac ggtcacgcta cgcgctcggc cccgggcggg
     1801 gtagtggacc aaggtctaat aggctcgccg gggcgcggcg actagagtcg gggcatgacg
     1861 tccccggcga cggacgcgca cgccttctcg gaggtgtttg gccagcggga agggccctac
     1921 cacctgcacg acgcgggagg tcgccccttg atccgggccg aatcgggcgc ccacgtgctg
     1981 ggagcgctgc tccggaagcg aacccgactg gtcttcctgg acgatgcggg atgtccggag
     2041 ctctccgcgc tgcggatcgc gtatccgacc cgggagtggt gcccacagcc gcccgagccc
     2101 accgcgtgcg agtgcgaaca gagcggctgc cacgggcggc tggtgatcac gctacacgac
     2161 gcgcacgcgg gcccgggctg gatgctgacg ttcaggggct aagagaggag cgaggaccat
     2221 ggagccgagc aaggtgaagg cgagtctggg gcagcgctgg agcgcggccc gcccgacgaa
     2281 gacgagcgtg ttctggttcg gggtgggcgc ggcggtggtg acgatgatcg tgggattcac
     2341 ctggggtgga tgggtgaccg ggggcaccgc tcgacggatg gcggaggcga gcggcgagga
     2401 ggcggtcgcc aagcggatgg cgccgatgtg cgtcgttcgc ttcaaggccg acccggaccg
     2461 gagcgagaag ctcaagcagc tccgggcagt cagcgcctgg gagcaggggg agttcgtcaa
     2521 gaagcagggc tgggcgacga tgccgggcga caaggagccc gagagccgga ccgccgatga
     2581 gtgcgtcaag ctgctcctgc tggtcagcta gcgcgacgac gtgacgctga cgcgggcgat
     2641 cgatgatctg gcgcgggccg ggttcggcga gcacttcggg gtggacggcg atgatctgcg
     2701 ctccttcacc agcggacggc ggtaccgggc ggccgaggtg gtgatccggg agttccaccg
     2761 gttcgagggg gtctcggatc ccgacgacat gtgcatcgtg tacgcgatcg aggcgcaggg
     2821 cggcgcacgg ggaaccctgg cggatgcgtt cggggtctac tccgacccgg tggtgagcgc
     2881 gtttttcgag aaggtcccga tccgcggggc ccggcggttc ggcggcgggc tcgacgagcg
     2941 gccgtgggag ttcggttctc cgggcgagat ccgacagcgg aagcgcatcc cggagcccgc
     3001 gcgcttcggt gcctaccgcc acggcgttcc gctcccggtt ccctccgacc cgtggcagga
     3061 cgagggtggg gagagcgggc cggggcagtg atcgtctacg cgccccgcgc ttgaccccgg
     3121 ccgcctgtcg aggcctcgcg ttgccggagg ggccggggca gactatagtg tcgactggat
     3181 gtcgggcgat tgggtcgtgt ggcaggtcgt cgtgggcgcg gtattcatcc tctcgctgct
     3241 gctgctcgcc atcgggctca tgttcgagcg ggcgtggcgg cgccgggcgg aggtcttgct
     3301 cggcgagcgg ctgcagttcg agaccttgct gtccgagcag gtcgcgacct tcagccgcat
     3361 ttcggcggcc gacgtcgatc gcgagatcca gcgggcgcta cgccggatcg ccgacctgct
     3421 cgcggtcgat tggggcaacc tgaccgagta ctcggatggc agccggaccg cccgggtcac
     3481 gcactggtgg gtggccgacg gggtggggcc gcagccggag gcgatcggat tcgaggagat
     3541 cccctgggtg gtgacgcgtc tgcgccgcgg agagctcgtc cggttctcga agatcgatga
     3601 gctccccgaa ggcgacgcga cggtcgaccg ggagacctac cgccgcctcg gcgtcaagtc
     3661 gcaggtcgcg gtgccgctga ccgtggaagg gacggtcgtc ggcgccctcg cgttcagcac
     3721 cgtcgggacc gagcgggcct ggcgggacga gttcg
//
LOCUS       DATMCV010000264         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_810591, whole genome shotgun sequence.
ACCESSION   DATMCV010000264 DATMCV010000000
VERSION     DATMCV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810591"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VNU03_06150"
     CDS             <1..433
                     /locus_tag="VNU03_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HWP74073.1"
                     /translation="RASGIDADARRDHPFAAYGELGLRVPVWESGDVKARTMVRIEEA
                     RESAALVRRALTSLPTGPLAAPLGPLPPFAPAFGLVEGWRGGILHWVMADGAGRLHRV
                     KIMDPSFLNWRPLSHALLHNIVPDFPLCNKSFNQSYSGNDL"
     gene            453..1046
                     /locus_tag="VNU03_06155"
     CDS             453..1046
                     /locus_tag="VNU03_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWP74074.1"
                     /translation="MKTGLIVLGVIILIVVGGIGWAIGINNQLVGQEQLVNEKWAQVQ
                     NVYQRRADLIPNLVETVKGFATQERTVLEEVTKARASVAGIKATPELINDPAAFQKFQ
                     QAQAELGGALSRLLVTVERYPDLKSNQNLLTLQSQLEGTENRIAVERMRYSEAVRDYN
                     TRIRLFPGSLVASLRGFKEKAYFEAAPGSQEAPKVKF"
     gene            1070..1849
                     /locus_tag="VNU03_06160"
     CDS             1070..1849
                     /locus_tag="VNU03_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWP74075.1"
                     /translation="MLRRLAPALLLLAVLAAPGAAALRIPPPPDHRVNDYAGALAPAD
                     RDRLEQALIAREATSRNQVVVAIFRSLEGESLEDYSIRLAKAWRIGQKGLDNGVIFLV
                     FLDDRKMRIEVGYGLEGSLTDAISSSILRDVVAPRFRAGQVGDGIGAGLDAIYRAIAG
                     TYQRPPGPNRGQPTHGFGWREGLGLLFVGLLIVVLVQNRMQQASLRRRGWTGGSTGWG
                     GPFIGGTGLGGGWGGGGGSSGGGGFSGGGGSFGGGGSSGSW"
     gene            1849..2301
                     /locus_tag="VNU03_06165"
     CDS             1849..2301
                     /locus_tag="VNU03_06165"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWP74076.1"
                     /translation="MARHPRWVRGFLAEADLDAVTRAIGAAELRTSAEIRVHLDHRCP
                     GEPMARAVAVFEHLGMHRTAARHGVLIYVSVGDHKLAVLGDQGIHERVGQVYWERLVD
                     AVLAHFREERPRDGLLHAVAEVGAVLARHFPRRPGDVNELSDQVSIDP"
     gene            complement(2374..>2742)
                     /locus_tag="VNU03_06170"
     CDS             complement(2374..>2742)
                     /locus_tag="VNU03_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74077.1"
                     /translation="IGSERLDAGALLLLSIGLPWGLSLDVNVGAAVLGQRRPEGFLPK
                     GVAAGSLSWAVTERLSTITELFFATKEERDSRANLRTTVALLYKLTPLMAVDAGMRTT
                     LVGPGPDWSAFVGFSARFGR"
BASE COUNT          393 a          953 c         1019 g          377 t
ORIGIN      
        1 gcgggcctcc gggatcgacg cggacgcgcg ccgcgatcat ccgttcgcgg cctacggcga
       61 gctgggtctg cgggtcccgg tgtgggagag cggcgacgtc aaggcgcgaa cgatggtgcg
      121 catcgaggag gcgcgggagt cggccgcgct cgtccgccgc gccttgacct ccctgcccac
      181 cggacccctc gccgcgccgc tcgggccgtt gccgcccttc gcacccgcgt tcgggctcgt
      241 ggaaggctgg cggggcggta tcctgcactg ggtgatggcc gacggggcgg ggcggctcca
      301 ccgggtcaag atcatggacc cgtcgttcct caactggcgg ccgctctccc acgcgctgct
      361 ccacaacatc gttccggact tcccgctctg caacaagtcc ttcaaccagt cgtattcggg
      421 aaacgacctc tgacgggggg tatactgcca gcatgaaaac cggactgatc gttctgggcg
      481 tgatcatcct gatcgtggtc ggcggcatcg gctgggcgat cgggatcaac aaccagctgg
      541 tggggcagga gcagctggtc aacgagaagt gggcccaggt gcagaacgtc taccagcgcc
      601 gcgcggacct gatcccgaac ctggtggaga ccgtgaaggg gttcgcgacc caggagcgga
      661 cggtgctgga agaggtgacg aaggcccgcg ccagcgtcgc cggcatcaag gccacccccg
      721 agctgatcaa cgacccggcc gcgttccaga agttccagca ggcccaggcc gagctgggcg
      781 gcgcgctcag ccggctcctc gtcaccgtcg agcgctatcc ggacctcaag tccaaccaga
      841 acctcctcac gctccagagc cagctcgagg gcaccgagaa ccgcatcgcg gtcgagcgca
      901 tgcgctacag cgaggcggtg cgcgactaca acacgcgcat ccggctgttc ccggggagcc
      961 tggtggcctc gctgcgcggc ttcaaggaga aggcctactt cgaggccgct cccggctccc
     1021 aggaagctcc caaggtgaag ttctaggcgg cgcgccgggc gggggaatcg tgctccgccg
     1081 cctcgctccc gcgctgctcc tgctcgccgt gctcgccgcc ccgggcgcgg cggccctgcg
     1141 catcccgccg ccccccgacc accgcgtcaa cgactacgcg ggggcgctcg cccccgccga
     1201 ccgcgaccgg ctcgagcagg ccctgatcgc gcgcgaggcc acgagccgca accaggtggt
     1261 ggtggcgatc ttccgctcgc tcgagggcga gagcctcgag gactactcga tccggctcgc
     1321 caaggcgtgg cgcatcgggc agaagggcct cgacaacggg gtcatcttcc tggtcttcct
     1381 cgacgatcgg aagatgcgca tcgaggtggg ctacgggctc gagggcagcc tgaccgacgc
     1441 catctcgtcc tccattcttc gagacgtcgt cgccccgcgt ttccgcgcgg gccaggtcgg
     1501 cgacggcatc ggggcggggc tcgacgcgat ctaccgcgcg atcgcgggga cgtaccagcg
     1561 gccgcccggc ccgaatcggg ggcagccgac ccatggcttc ggctggcggg aagggctcgg
     1621 gctgctcttc gtcggcctcc tgatcgtcgt gctcgtgcag aaccggatgc agcaggcgtc
     1681 gctccggcgc cgcgggtgga ccggcgggtc gaccggctgg ggtggtccct tcatcggcgg
     1741 gaccggcctg ggcgggggat ggggcggcgg cggtggcagc agcgggggcg gcgggttcag
     1801 cgggggcggg ggcagcttcg gcggcggcgg ctcgagcggg agctggtgat ggcgcgtcat
     1861 ccgaggtggg tgcgcgggtt cctcgccgag gccgatctcg acgcggtgac gcgggccatc
     1921 ggggcggcgg agctgcgcac gtccgcggag atccgggtgc acctcgacca tcgctgcccg
     1981 ggcgagccga tggcgcgggc ggtcgcggtc ttcgagcacc tcggcatgca ccggaccgcg
     2041 gcccgccacg gcgtgctgat ctacgtgagc gtgggggacc acaagctggc cgtgctgggc
     2101 gaccagggga tccacgagcg ggtcgggcag gtctactggg agcggctcgt cgacgcggtg
     2161 ctcgcgcact tccgggagga gcgcccgcgc gacgggctgc tgcacgcggt ggccgaggtg
     2221 ggcgcggtgc tcgcccggca tttcccgcgg aggccggggg acgtcaacga gctgtcggat
     2281 caggtgagca tcgatcccta gcggccgaag cggggcccta gcggccaaag cgggccctag
     2341 cggccgaagg ggccctagcg gccgaaacgg gccctagcgg ccaaagcggg cgctgaagcc
     2401 gacgaaggcc gaccagtcgg gcccgggacc gacgagcgtg gtgcgcatcc cggcgtcgac
     2461 cgccatgagc ggcgtgagct tgtagaggag cgccaccgtc gtccggagat tcgcgcggct
     2521 gtcgcgctcc tccttggtgg cgaagaagag ctcggtgatc gtcgacaggc gctcggtcac
     2581 cgcccacgag agcgatcccg ccgccacccc cttcggcagg aacccctccg ggcgccgctg
     2641 accgagcacg gccgccccca cgttgacgtc gaggctcagc ccccacggca ggccgatgct
     2701 cagcagcagg agggcccctg cgtccagccg ctcgctaccg at
//
LOCUS       DATMCV010000265         2142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_748636, whole genome shotgun sequence.
ACCESSION   DATMCV010000265 DATMCV010000000
VERSION     DATMCV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748636"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VNU03_06175"
     CDS             <1..532
                     /locus_tag="VNU03_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWP74078.1"
                     /translation="GHAKASTPEERRRLFLSRKSQFRRLFERANAVLENLEQATIAAV
                     NGHAVGGGWGLALACDFRIAAAEAQFWIPEVDLGVPLGVGTTTRFVRMVGPARAKEII
                     MGCARYSAADAQAWGLVHRVVPGAGLAAAVADYAAGLAKKPFRPMAEVKARINAIART
                     GIPEINAMTEGFLDRE"
     gene            complement(495..857)
                     /locus_tag="VNU03_06180"
     CDS             complement(495..857)
                     /locus_tag="VNU03_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP74079.1"
                     /translation="MARTPRPGRPVRGSQTGRPIMALMDLLGRRWTLRVIWELRGGRL
                     TFRALQEACGGLSPTVLNQRVRELRESGLLEAEGAAGYGLTPLGRELVDQVLPLVAWS
                     TRWAAALTRGRGTPRSSR"
     gene            949..1554
                     /locus_tag="VNU03_06185"
     CDS             949..1554
                     /locus_tag="VNU03_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP74080.1"
                     /translation="MSTPRITPLEPPFPEPVQAALDRIMPKGVPPLALFRTLARNERV
                     FQRVMAGGLLDRGSITLREREIVIDRTCARCGSEYEWGVHVAFFAERVGFSPEQVAAT
                     TGGDATAFSERERLLLRLVDELHDRALVSDELWAALRAHFAEEQLIELVALAGFYHLI
                     SFVTNALRIPLESYGARFPHSPAPAGAGGGRSPRASRGAGR"
     gene            complement(1448..>2142)
                     /locus_tag="VNU03_06190"
     CDS             complement(1448..>2142)
                     /locus_tag="VNU03_06190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP74081.1"
                     /translation="QVLRNPYTWPPFLCFFFLYAAMGNFFLWSVPFLRDVYGLTTTRA
                     ALYASLPSMALLASAPFTGYLSDRVLRRRKLPYTVLASGQFLVWLAFVLTLGTLPLPG
                     VCLLFLLLGVMGGAFVLTWPLGAEVNPPGLAGIAVAVTNLGGFVGAALTQGPIGAVLD
                     AGWMGAMAEGARVYPVASYRAGFALCALFVLIGAAITCLLHETRGANVHHQLRPARAS
                     AGSGPRKTRGES"
BASE COUNT          331 a          765 c          783 g          263 t
ORIGIN      
        1 gggccacgcc aaggcgtcca cgccggagga gcggcggcgc ctcttcctct cgcgcaagag
       61 ccagttccgg cgcctcttcg agcgtgccaa cgcggtcctc gagaacctcg agcaggcgac
      121 catcgcggcg gtgaacggcc acgcggtggg gggcgggtgg ggcctggccc tggcctgcga
      181 cttccggatc gcggccgccg aggcccagtt ctggatcccg gaggtggacc tgggcgtccc
      241 gctcggagtg gggaccacga cccgcttcgt ccggatggtc gggcccgcgc gcgccaagga
      301 gatcatcatg ggttgcgcgc gctactccgc cgccgacgcc caggcgtggg gcctggtcca
      361 ccgggtggtg ccgggcgccg ggctcgccgc cgcggtcgcc gactacgcgg cggggctcgc
      421 gaagaagccg ttccggccga tggcggaggt gaaggcgcgc atcaacgcga tcgcgcgcac
      481 cggcatcccc gagatcaacg cgatgaccga ggggttcctc gaccgcgagt gagcgcggcg
      541 gcccagcgcg tcgaccaggc gaccagcggc agcacctgat cgaccagctc gcgcccgagc
      601 ggtgtgagcc cgtagcccgc ggcgccctcc gcctcgagca gcccgctctc ccgcagctcg
      661 cgcacccgct ggttcagcac ggtggggctc agcccaccgc acgcttcctg cagggcccgg
      721 aaggtcagcc ggccgccccg cagctcccag atcacccgca gggtccagcg ccggccgagc
      781 aggtccatga gggccatgat cggccgcccg gtctgcgagc cccgcaccgg ccgtcccggc
      841 cggggagtcc tggccatcgg tcgcgatctc cttgcgctat cgaaacagta gcggtagcat
      901 cgtagctact gaaatgatag caccacccgg acccggagga tcgacgacat gagcaccccg
      961 cgcatcaccc cgctggagcc cccgttcccc gagccggtcc aggccgcgct ggaccgcatc
     1021 atgccgaagg gcgtcccccc gctcgccctc ttccgcacgc tggcccgcaa cgagcgcgtg
     1081 ttccagcgcg tgatggcggg cggcctcctc gaccggggct cgatcacgct gcgcgagcgc
     1141 gagatcgtca tcgaccggac ctgcgcccgc tgcggctccg agtacgagtg gggcgtgcac
     1201 gtggcgttct tcgccgagcg ggtggggttc agcccggagc aggtggccgc caccaccggg
     1261 ggggatgcca ccgccttctc ggagcgagag cgcctgctcc tccggctggt ggacgagctg
     1321 cacgaccggg cgctggtgag cgacgagctg tgggcggcgc tgcgggcgca cttcgccgag
     1381 gagcagctca tcgagctggt ggcgctggcc ggcttctacc acctgatctc gttcgtcacc
     1441 aatgcgctca ggattcccct cgagtcttac ggggcccgct tcccgcactc gcccgcgccg
     1501 gccggagctg gtggtggacg ttcgccccgc gcgtctcgtg gagcaggcag gtgatcgcag
     1561 ccccgatcag gacgaagagc gcgcagagcg cgaagccggc gcggtaggac gcgaccgggt
     1621 agacgcgcgc tccctcggcc atcgcgccca tccagcccgc gtcgagcacc gctccgatcg
     1681 gaccctgggt gagggcggcg cccacgaagc caccgagatt cgtcaccgcc accgcgatgc
     1741 cggccagacc gggcgggttc acctcggccc cgagcggcca ggtgaggacg aaggcgccgc
     1801 ccatcacgcc cagcaggagg aagaggaggc agaccccggg gagcggcagc gtgccgaggg
     1861 tcagcacgaa cgcgagccac acgaggaact ggccggaggc cagcacggtg tacgggagct
     1921 tccggcggcg caggacccga tcggagaggt agccggtgaa cggggcggag gcgagcagcg
     1981 ccatcgacgg caatgaagca tagagcgcgg ccctggtggt ggtgaggccg tagacgtcgc
     2041 gcaggaacgg cacgctccag aggaagaagt tccccatcgc ggcgtagagg aagaagaagc
     2101 agaggaacgg cggccaggtg tacgggttcc ggagcacctg ga
//
LOCUS       DATMCV010000266         8202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_281569, whole genome shotgun sequence.
ACCESSION   DATMCV010000266 DATMCV010000000
VERSION     DATMCV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281569"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VNU03_06195"
     CDS             complement(<1..508)
                     /locus_tag="VNU03_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP74082.1"
                     /translation="MESSDEELCRAVAERKPGAFDLLVERYQERAYRIAWSVVRDREE
                     ARDCSQEAFLRLHESAGSFAGQSKFSTWFYRILVNCCLDQKRRGRGWRQLLGWRDRRD
                     DGPDEPDPVEQAPAPFADPADHVDAERRMSRVWALVEELSPQQRAAVTLSCREGMATK
                     DIAAVLSLS"
     gene            complement(623..1084)
                     /locus_tag="VNU03_06200"
     CDS             complement(623..1084)
                     /locus_tag="VNU03_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74083.1"
                     /translation="MDKHMGWLVRTLAVAATGVTVGLLSTAASAQEIRNDVQDLRQDR
                     KEIRQDNREIGGDRRELRGDTKEIQGDRRELQGDRRDLRDAQKSGDPNAVKDAKQDLR
                     GDRRDLRSDVKDRRGDGRDLRQDKRDRRGDVKDLRHDRRDLRKDVAEKSAK"
     gene            1229..1420
                     /locus_tag="VNU03_06205"
     CDS             1229..1420
                     /locus_tag="VNU03_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74084.1"
                     /translation="MWIGAAVLAILVVAAVPAFIEAGGASLRGLGWLIVATGAAVLLI
                     DLTDRLTEWVERRRWGRRR"
     gene            complement(1487..2485)
                     /locus_tag="VNU03_06210"
     CDS             complement(1487..2485)
                     /locus_tag="VNU03_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWP74085.1"
                     /translation="MSSYARLGKILLGLVVSAGLLLYFFWDVDLRVVGARLRETLWGF
                     LALSVALNILSLWARARRWRYLFAPGAQAGHLFRALMVGYMGNNLLPLRAGEIVRVYV
                     ASRHGPRFWTAFATVVVERVLDGLALGLLVAGVLLIVPVPREMRWSILVFLAVDLLGM
                     LLLAAIAVAPGACRALIQVLFHRFGWIERRMLGMLDTMTEGLRGLRAPRHLLPIAVYS
                     VAIWLFLALSVWTALHAAHLDLPLAAAWTVLAFLGLGVSLPSSPGFVGVVQAATVLAL
                     ALFDVPRTEALSFSLLMHASQFLPVTAVGLVFLFLEHVRLTDATRVDVGRVVSSER"
     gene            complement(2526..3824)
                     /locus_tag="VNU03_06215"
     CDS             complement(2526..3824)
                     /locus_tag="VNU03_06215"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWP74086.1"
                     /translation="MNEIKIALLGLGTVGCGVAKILQSHGAELAERAGCRLAFGRVAD
                     ADLTRPRDGLDLARLPLTADAGQAIDDPSVQIVIELIGGLEPARTFILRALQAGKHVV
                     TANKALLAHHGTELFEEARRHRVMLGFEAAVAGGIPLIRAVKDGLPANRILSTFGIVN
                     GTCNYILSKMTDEGRDFGEVLKEAQAHGYAEADPTLDIEGLDSAHKLQILATLAFRTA
                     VDLKDIYTEGITAVGQQDVVNATELGYRIKLLAIAKASEGALEVRVNPTMIPAGSPLA
                     AVSGVFNAVFITGDNVGDLMFYGRGAGQLPTASAVWSDTIDIARRVAHGIPALDTDLP
                     TIGRNPLPLRPVDDIRSAYYLRVMALDRPGVLAQVAGILGQHDISLVSVLQKVRARHE
                     AVPVVMMTHEARERDMRAALAAIDKLPVVAARTTMIRVEA"
     gene            complement(3890..5062)
                     /gene="alaC"
                     /locus_tag="VNU03_06220"
     CDS             complement(3890..5062)
                     /gene="alaC"
                     /locus_tag="VNU03_06220"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HWP74087.1"
                     /translation="MDEFYRIKRLPPYVFAIVNDLKSKARAKGEDIIDLGMGNPDQET
                     PKHIVNKLVEAARNPRNHRYSASKGITRLRKAVTTWYRTRYGVELDAETEAIVTIGAK
                     EGMAHLALAVLQPGDGVLVPNPTYPIHSYSVVIADGDLRSVPLIPGDDFFARLQETAR
                     LSWPKAKLLILSFPHNPTTLCVDRDFFVKVVDFAREHQLMVVHDFAYADFAYDGYKPP
                     SFLEVPGAKDVGVEIFSLSKSYNMPGWRVGFVCGNSRMVNALARIKSYLDYGAFQPIQ
                     IAAIIALEGEQGCVGDIVELHRKRRDVLVDGLNKIGWSVPKPKGTMFVWAPIPEAFRS
                     MGSLEFSKLLIQECKVAVSPGIGFGEYGDGHVRFALVENAQRIRQALRGLKTLTGR"
     gene            5339..5749
                     /locus_tag="VNU03_06225"
     CDS             5339..5749
                     /locus_tag="VNU03_06225"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWP74088.1"
                     /translation="MFGRRTKGPRRGDLTAFIDEGSEMEGRYTFRGTVMLNGKFKGEI
                     SSGDTLIIGERAVVEADVRAGRVTVSGEVTGNLRATERVELKRTARVYGDVEAPIVVV
                     EEGVIFEGHCRMAKPTPVTEVAAARDLAVVPLKR"
     gene            5752..6675
                     /locus_tag="VNU03_06230"
     CDS             5752..6675
                     /locus_tag="VNU03_06230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWP74089.1"
                     /translation="MGKPRQFNLLMVRDDGSRVLRLSVPGWVVGAVVGGLVVTLIGLA
                     FIYPDYATLRHQRGTFATLSARLHTQQALLDAAQSRLREIRGEIDSWRDLHAKIWEPF
                     GPEAGPAKRGVGIGGGNAAAEPAEPDRAPVREDMDRLLGVVKEEGESIRTLERFLGRA
                     SRVLASLPSRWPVRGPVNSDFGQRRSPWAPNSEFHSGIDIGAAIGTPVKSPAPGTVVF
                     AGQHPEYGVTLIIDHGNDTKSLYGHLSRLSVGLDQLVQRGETVALTGNTGRSSGPHLH
                     YEIQVKGQPVNPHSYIWEEPGPAVARSTARR"
     gene            complement(6694..7506)
                     /locus_tag="VNU03_06235"
     CDS             complement(6694..7506)
                     /locus_tag="VNU03_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP74090.1"
                     /translation="MPTVTANGVRLFYEEAGAPDAPPLVLIMGWGGDHTAWALQMPAL
                     GADYRVIALDNRGAGQSEVPEGPYTIPGMAADVVGLMDALGVARAHICGASMGGMIAQ
                     ELALRHPERVRTLQLHCTAPIIDGYGHFLVDTLLAVKARGDREENVRAIMPWILCRKT
                     MVERPEFIRFWIERGLTYPYPITFEGLSRQADAIKGHDTLARLGEIRMPTLLTTGTED
                     ILVPPVSSRELHARIPGAELVEIGDAGHLHFIEQGEKFNAVCLEFLRKHRGA"
     gene            complement(7512..>8202)
                     /locus_tag="VNU03_06240"
     CDS             complement(7512..>8202)
                     /locus_tag="VNU03_06240"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWP74091.1"
                     /translation="RGRVPVAVGVSASGTHLATAFARMAREHGADALMVAPPTGLKNL
                     DTVAEHYRIVAAATGLPIVLQDEPVTTQVNMPAPFIAQVCADLPAIQAVKLEEPPTLP
                     KITRLRALFGSRVAIFGGLGGVYFFEELSRGADGAMTGFPYPEALRAIREHFVAGRRD
                     EARALFYRWLPLIRYESQPGATPGTAVGIRKEILRRRGWIASALVRPPAPALDAGTHA
                     ELAEILAAVGA"
BASE COUNT         1197 a         2871 c         2957 g         1177 t
ORIGIN      
        1 ccgacaggct cagcacggcg gcgatgtcct tggtcgccat tccctcgcga caggagagcg
       61 tcaccgccgc gcgctgctgc ggcgagagct cttccaccag cgcccacacc cggctcatgc
      121 gtcgctccgc gtccacgtga tccgcggggt ccgcgaacgg cgcgggcgcc tgctcgaccg
      181 gatccggctc gtcgggcccg tcgtcgcgcc ggtcgcgcca gcccagcagc tgccgccagc
      241 cccgcccgcg acgcttctgg tcgaggcagc agttgaccag aatgcggtag aaccacgtcg
      301 agaacttgga ctgcccggcg aaggaaccgg ccgactcgtg caggcgaagg aacgcttcct
      361 gggagcagtc gcgggcttcc tcccgatcgc gcacgacgga ccaggcgatg cggtaggccc
      421 gctcctgata gcgctcgacc aggaggtcga aggcccccgg cttccgctcc gccaccgcac
      481 gacacagctc ctcatcgctc gactccacgg gcgcctctcg ccccgatctt cccatacgcg
      541 cgcattcgac tccaagcctc ccgccgctcg cgggtgggct ccgggagcga tcgcagccgc
      601 tcccggagcc cggcccaccg ctctacttcg cgctcttctc cgcgacgtcc ttccgcagat
      661 cgcgccgatc gtggcgcagg tccttcacgt cgccgcgccg gtcgcgcttg tcctgccgca
      721 gatcgcgccc gtctccccgc cggtccttca cgtcgctccg gagatcgcgg cggtcgcccc
      781 gcaggtcctg cttggcgtcc ttgaccgcgt tcgggtcgcc ggacttctgg gcgtcccgga
      841 ggtcgcgccg gtcaccctgc agctcgcgcc ggtcgccctg gatctccttc gtgtcgccgc
      901 gcagctcgcg ccggtcaccg ccgatctcgc ggttgtcctg gcggatctcc ttccgatcct
      961 gccgcaggtc ctggacgtcg ttccggatct cctgggccga ggcggccgtg gagagcagcc
     1021 cgaccgtcac tccggtcgcc gccaccgcca gcgtccgtac cagccaaccc atgtgcttgt
     1081 ccatcggtat cctcctggat ttgcagggat tttggagttg agccccgggg tgctgcgatc
     1141 actggatgag acgccgtggc ccgtgcccgc gtttacatcc gcttattctt cttggcgttc
     1201 cggggggtgg ctctctacgc ttgctggcat gtggatcggt gcggcggtcc tggcgatcct
     1261 ggtggtggcg gcggtgccgg ccttcatcga ggccggcggc gcgagtctgc gcgggctcgg
     1321 ctggctgatc gtcgcgaccg gggccgcggt gctgctgatc gacctgaccg accggctcac
     1381 cgaatgggtg gagcgccggc ggtggggccg tcgccgctag cttcgccgcc tcgcccgctc
     1441 ccgctcgcgc ctcaccgctt cccgctcccg ttcaccgctc gtcccttcac cgctctgaag
     1501 acacgacccg gcccacgtcg acgcgggtgg cgtccgtcag ccggacgtgc tccaggaaca
     1561 ggaacaccag gcccaccgcg gtcaccggca ggaactgcga ggcgtgcatg aggagcgaga
     1621 aggagagcgc ctcggtgcgc ggcacgtcga agagggcgag cgcgagcacc gtcgcggcct
     1681 ggaccacgcc gacgaagccg gggctcgacg gcaggctcac gcccaggccg aggaaggcga
     1741 gcacggtcca cgcggcggcg agcgggagat ccaggtgggc cgcgtggagc gcggtccaca
     1801 ccgacagggc caggaagagc cagatcgcca ccgagtaaac cgcgatcggc agcaggtggc
     1861 ggggcgcgcg aaggccgcgc agcccctcgg tcatggtgtc gagcatgccg agcatccgcc
     1921 gctcgatcca gccgaaccgg tgaaagagga cctggatcag cgcgcggcac gcgccggggg
     1981 cgaccgcgat cgcggccagc agcagcatgc cgagcaggtc cacggcgagg aagacgagga
     2041 tcgaccagcg catctcgcgg ggcaccggca cgatcagcag gaccccggcc acgagcaggc
     2101 ccagcgcgag cccgtcgagc acgcgctcca ccaccacggt ggcgaaggcg gtccagaacc
     2161 gcgggccgtg gcgcgacgcc acgtagaccc gcacgatctc tccggcccgg agcgggagca
     2221 ggttgttgcc catgtagccc accatcaggg cccggaagag atggccggcc tgcgcccccg
     2281 gcgcgaacag gtagcgccac cgccgggcgc gggcccacag ggagaggatg ttgagcgcga
     2341 cgctcaaggc caggaagccc cagagggtct cgcggagtcg cgcgccgacg acgcggaggt
     2401 ccacgtccca gaagaagtag agcaggagcc cggcgctcac cacgaggccg aggaggatct
     2461 tccccagtcg tgcgtacgac gacatgggcg ggcggctcag cgcccgggcc ggcccgaccg
     2521 cccgctcaag cctccacccg gatcatggtg gtccgggcgg ccaccaccgg cagcttgtcg
     2581 atggcggcga gggccgcgcg catgtcccgc tcgcgggcct cgtgcgtcat catcaccacc
     2641 ggcaccgctt cgtggcgcgc gcgcaccttc tggagcaccg agaccagcga aatgtcgtgc
     2701 tgaccgagga tgcccgccac ctgggcgagg acgccgggcc gatccagcgc catcacgcgc
     2761 aggtagtagg cggagcggat gtcgtcgacc ggccgcaggg gcagcgggtt gcgcccgatc
     2821 gtggggagat cggtgtcgag cgcggggatg ccgtgggcca cccggcgcgc gatgtcgatg
     2881 gtgtcggacc agaccgcgga ggcggtgggg agctggccgg ccccgcggcc gtagaacatg
     2941 aggtcgccca cgttgtcgcc ggtgatgaag accgcgttga acacgccgga gaccgcggcg
     3001 agcggggagc cggccgggat catggtcggg ttcacccgga cttccagggc tccctcggag
     3061 gccttggcga tcgcgagcag cttgatgcgg tagccgagct cggtcgcgtt caccacgtcc
     3121 tgctggccga ccgcggtgat gccctcggtg tagatgtcct tcagatcgac cgcggtgcgg
     3181 aaggccaggg tggccaggat ctgcagcttg tgggccgagt cgagcccctc gatgtcgagg
     3241 gtcggatccg cctccgcgta gccgtgcgcc tgcgcctcct tgaggacctc tccgaagtcg
     3301 cgtccctcgt cggtcatctt ggagaggatg taattgcagg tgccgttgac gatcccgaag
     3361 gtcgagagga tgcggttcgc gggcagcccg tccttcaccg cgcggatgag cgggatgccg
     3421 cccgccaccg cggcctcgaa cccgagcatg acccggtggc gccgggcctc ctcgaacagc
     3481 tcggttccgt ggtgggcgag cagcgccttg ttggcggtga ccacgtgctt gcccgcctgc
     3541 agggcccgca ggatgaacgt ccgcgccggc tcgagcccgc cgatcagctc gatcacgatc
     3601 tgcaccgagg gatcgtcgat ggcctggccc gcgtcggcgg tgaggggcag ccgcgccagg
     3661 tcgagcccgt cgcggggccg ggtgaggtcc gcgtccgcca cgcgcccgaa ggcgaggcgg
     3721 catcccgcgc gctcggccag ctcggcgccg tggctctgga ggatcttggc cacgccgcag
     3781 ccgaccgtgc cgaggcccag aagggcgatc ttgatctcgt tcatgggtcc atttcgaggc
     3841 cgcccgggcg gccgaccccc ggggaagccc gtgagccgct cccggcccgc taccggccgg
     3901 tgagggtctt gaggccgcgc agggcctgtc ggatgcgctg cgcgttctcg acgagggcga
     3961 agcggacatg gccgtcgccg tactcgccga agccgatgcc cggggacacc gccaccttgc
     4021 actcctggat gagcagcttg gagaactcga gcgagcccat gctccggaag gcctccggga
     4081 tcggggccca cacgaacatg gtgcccttgg gcttggggac ggaccagccg atcttgttga
     4141 ggccgtcgac gagcacgtcg cggcgcttgc ggtgcagctc gacgatgtcg cccacgcagc
     4201 cctgctcgcc ctcgagcgcg atgatcgccg cgatctggat gggctggaag gcgccgtagt
     4261 cgaggtacga cttgatgcgg gccagcgcgt tgaccatccg ggagttgccg cacacgaagc
     4321 ccacccgcca gcccggcatg ttgtaggact tcgacagcga gaagatctcg acccccacgt
     4381 ccttggcccc cggcacctct aggaaggagg ggggcttgta gccgtcgtag gcgaagtcgg
     4441 cgtaggcgaa gtcgtgcacc accatcagct ggtgctcgcg ggcgaagtcg acgaccttga
     4501 cgaagaagtc gcggtccacg cagagggtgg tcgggttgtg cgggaaggag aggatcagga
     4561 gcttggcctt gggccaggag agccgggccg tctcctgcag ccgggcgaag aagtcgtcgc
     4621 ccgggatgag cggcaccgag cgcaggtcgc cgtccgcgat caccaccgag taggagtgga
     4681 tggggtaggt cgggttgggc accagcaccc cgtcccccgg ttggagcacc gcgagggcca
     4741 ggtgggccat gccctccttg gccccgatgg tgacgatggc ctcggtctcc gcgtccagct
     4801 cgaccccgta gcgggtccgg taccaggtcg tgaccgcctt ccgcagccgg gtgatcccct
     4861 tggaggccga gtatcggtgg ttgcgggggt tgcgggccgc ctcgaccagc ttgttgacga
     4921 tgtgcttggg ggtttcttgg tcgggattgc ccatcccgag gtcgatgatg tcctcgccct
     4981 tggcgcgcgc cttcgacttc aggtcgttga cgatcgcgaa gacgtagggc gggaggcgct
     5041 tgattcgata gaactcgtcc atggtttcgt ctccagggac ctcgatcata ctgtgccgct
     5101 cgcgcgagtg tcaacgaagt cgccctgatg ccgcgcgggg atagcgcgca cgccggccgg
     5161 aggccggacg ggggttgaca ccattttaaa gggcgtgcta gaagagcctt accgggcgaa
     5221 cccgtaacca acacccacct tccagcacgc tccgagcgaa cagtcgagtt tcccgacccc
     5281 cgcgggggtc ccagggcccg gcaggacggc agtcaagcgc gtcgagggga cgagtcttat
     5341 gttcggacga aggaccaagg gaccgcggcg gggcgatctg accgccttca tcgacgaggg
     5401 ttcggagatg gagggccggt acaccttccg gggcaccgtc atgctcaacg gcaagttcaa
     5461 gggcgagatc agctccggcg acaccctgat catcggggag cgcgcggtcg tcgaggccga
     5521 cgtccgggcc ggccgggtga cggtgagcgg cgaggtcacg ggcaacctgc gggccaccga
     5581 gcgggtggag ctcaagcgca ccgcgcgcgt ctacggcgac gtcgaggccc cgatcgtggt
     5641 ggtggaagag ggcgtgatct tcgagggcca ctgccgcatg gccaagccga ccccggtgac
     5701 ggaggtcgcc gccgcgcggg acctcgcggt cgtgccgctc aagcggtgac gatgggcaag
     5761 cccaggcagt tcaacctgct gatggtgcgc gacgacggca gccgtgtgct gcggctgtcg
     5821 gtgccgggct gggtggtggg cgcggtggtg ggcgggctcg tggtgaccct gatcgggctc
     5881 gcgttcatct atcccgacta cgccaccctc cgccaccagc gcggcacctt cgcgaccctg
     5941 agcgcgcggc tgcacaccca gcaggccctc ctcgacgcgg cccagtcgcg cctgcgcgag
     6001 atccgcggcg agatcgacag ctggcgcgac ctgcacgcca agatctggga gccgttcggg
     6061 ccggaggccg gcccggccaa gcgcggcgtc ggcatcggcg ggggcaacgc cgccgcggag
     6121 cccgcggagc ccgatcgcgc cccggtccgc gaggacatgg accggctgct cggggtcgtc
     6181 aaggaagagg gcgagtcgat ccggacgctc gagcggttcc tcggccgcgc cagtcgcgtg
     6241 ctcgcctcgc tgccgtcgcg ctggccggtg cgcggcccgg tcaactccga cttcggccag
     6301 cgccgctcgc cgtgggcgcc caactccgag ttccacagcg gcatcgacat cggggccgcg
     6361 atcggcaccc cggtgaagtc gcccgccccc ggcaccgtgg tgttcgcggg ccagcatccg
     6421 gagtacggcg tcaccctcat catcgaccac ggcaacgaca ccaagtcgct ctacggccac
     6481 ctgtcgcgac tgagcgtcgg gctggaccag ctggtgcagc ggggggagac ggtggcgctc
     6541 accggcaaca ccggacgctc ctccggcccg cacctgcact acgagatcca ggtcaagggc
     6601 cagccggtga acccgcacag ctacatctgg gaagagccgg gaccggccgt cgccaggtcc
     6661 accgcgcgac gctgaccggc cggacgcggg ccgtcaggcc ccgcggtgct ttcgcaggaa
     6721 ctccaggcag accgcgttga acttctcccc ctgctcgatg aagtgcaggt gtcccgcgtc
     6781 gccgatctcg accagctccg cccccgggat gcgcgcgtgc agctcgcggg aggagacggg
     6841 gggaaccagg atgtcctcgg tgccggtggt gagcagcgtc ggcatccgga tctcgcccag
     6901 gcgcgcgagc gtgtcgtggc ccttgatggc gtccgcctgc cgcgacaatc cctcgaaggt
     6961 gatcggatag ggataggtca ggccgcgctc gatccagaag cggatgaact ccggccgctc
     7021 gaccatggtc ttgcggcaga ggatccaggg catgatcgcg cgcacgttct cctcgcgatc
     7081 gccccgcgcc ttcaccgcga gcagcgtgtc gaccaggaag tgtccgtagc cgtcgatgat
     7141 gggcgcggtg cagtgcagct ggagcgtgcg cacccgctcc gggtgccgca gggccagctc
     7201 ctgcgcgatc atgccgccca tcgaggcgcc gcagatgtgc gcgcgcgcga cgccgagggc
     7261 gtccatcagg cccaccacgt ccgcggccat gccgggaatc gtgtacggcc cctccggcac
     7321 ctcgctctgt cccgcgccgc ggttgtcgag ggcgatcacc cggtagtcgg cgcccagggc
     7381 gggcatctgc agggcccacg cggtgtgatc gccgccccag cccatgatca gcaccagggg
     7441 tggggcgtcc ggcgcgcccg cctcctcgta gaagagacgg acgccgttcg cggtgacggt
     7501 gggcatcgcg atcaggcccc caccgcggcc aggatctcgg ccagctcggc gtgggtgccc
     7561 gcgtcgaggg cgggagccgg cgggcgcacc agggcggagg cgatccaccc ccggcgccgc
     7621 aggatctcct tgcggatgcc gaccgcggtg ccgggggtcg ctcccggctg gctctcgtag
     7681 cggatgaggg gcagccaccg gtagaagagc gcgcgcgcct cgtcgcggcg cccggccacg
     7741 aagtgctcgc ggatcgcccg cagcgcttcc gggtagggga agccggtcat ggccccgtcc
     7801 gcgccgcgcg agagctcctc gaagaagtag acgccgccga gcccgccgaa gatcgcgacc
     7861 cggctcccga agagcgcgcg gagccgcgtg atcttgggca gggtcggcgg ctcctcgagc
     7921 ttgaccgcct ggatcgcggg gaggtcggcg catacctggg cgatgaacgg cgccggcatg
     7981 ttcacctggg tggtgaccgg ctcgtcctgc agcacgatcg gcaagccagt ggccgccgcg
     8041 actatgcggt agtgctccgc caccgtgtcg aggttcttga gcccggtcgg cggcgcgacc
     8101 atgagcgcgt ccgcgccgtg ctcgcgcgcc atgcgggcga aggcggtggc caggtgggtg
     8161 ccagaggcgg acacccccac cgccaccggc acgcggccgc gc
//
LOCUS       DATMCV010000267         6489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_347610, whole genome shotgun sequence.
ACCESSION   DATMCV010000267 DATMCV010000000
VERSION     DATMCV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347610"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1372
                     /locus_tag="VNU03_06245"
     CDS             173..1372
                     /locus_tag="VNU03_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74092.1"
                     /translation="MRMHRRIATLATLAFAAALCGGGAVQAEDKTLTIGLLGPLSGGA
                     ASYGVELMRGAELRVEEINKGGGLKVGADVYRIKLVSYDHKAQAADTATATNKLIFQD
                     KVKYIIGNAVGATCNAAQTITEPNKVMFAFVCWGTNNLAPEKPYSFRSMLSQWELAEP
                     FYRWVKENHPTIKRVAAISPNDTSGKDTNTAVVKALKALGFEVAADEYYERGTKDFYP
                     VLTKILAQKPDMIDVAAAPPGEAGLILKQAMELGFKGAKGWTAGINPFTIISVAGPEA
                     AEGVWSPANINVKGDHVSPAVRKFGETYEKRYGEVPGAIAVANYAAFDVFTQAMQKAG
                     SIDTDRVLAVITKEQFQTVWGPLALGGKETYGIDRQFLYPLVISEVRQGKVVDIAQVL
                     PAALKKK"
     gene            1431..2291
                     /locus_tag="VNU03_06250"
     CDS             1431..2291
                     /locus_tag="VNU03_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74093.1"
                     /translation="MPIELLIQALLNGFGLAVVYILVALGLTLIFSILEVINFAHGEF
                     YMLGGFAAYYLSAVGGLNYVATLVLAMLLVGLAGVVAERLVFRHLRGKTLNAFIVSLG
                     LLWVLQATAQLSFGVLDKSVPSAVSGIVRLGGVIVSRERMFVILTAVALIVGLHLFLK
                     FTRTGQAMRAVAQDAEAAALQGVNIELTSALGFGIGCALAGAAGALLAPIFAVSPTMG
                     ALPVVKAFIIIIVGGMGSLPGAVLGGLLLGAVEGVGTLFMSSAAVNILGFLMVIAILL
                     LRPRGLLGAA"
     gene            2281..3297
                     /locus_tag="VNU03_06255"
     CDS             2281..3297
                     /locus_tag="VNU03_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74094.1"
                     /translation="MPPEPRRVDGWIGFGVLLVAGVSVPLLTRDSYHLDVATVILRNL
                     LLTMSLRYVMSTGQLNMAHVSFMGIAAYASALLVTRAGWSFWAALAVAPLLAALAAVP
                     MGRVALRVSGPYYFLISFAFLEVLRLVFNNFFVDYLGGPSGLVEIPPPHPLLLTGGAV
                     TFIGKLAQYWLMLVLFLASAAVLIRLECSRVGLVNGAIRQGELLAETLGVNVPRYKLG
                     AFVLGAFFAGVAGVFFAHSQRVLHSSDFGLEPMVLLVVFTVIGGAGSVWGPVVGTLVL
                     AVASELLRELHHYELLVYGLVLMVVMLCFPDGLVALPGRVARRFGWGRAKDGASTLGA
                     GVAP"
     gene            3287..4054
                     /locus_tag="VNU03_06260"
     CDS             3287..4054
                     /locus_tag="VNU03_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74095.1"
                     /translation="MLLELREVGKRFGGLQAVAAFSMTVRAGTIHGLIGPNGAGKSTV
                     FNLITGVLPVTSGEIHFKGERISGLRPSEICRRGIARTFQATTFFREFTVLDNVMAAS
                     HLAVERGFVKTLLATAGYRAREAAALARAQDLLDAVRLQDVAGRRAGTLPHRDQKALS
                     LALVLATGAGLVILDEPLAGLTTQEGEDAMRTLQALRADGKTILLVEHDMRAVMGLCD
                     VITVLDHGVRIAEGVPRDIQANPAVIEAYLGAEDTRA"
     gene            4041..4754
                     /locus_tag="VNU03_06265"
     CDS             4041..4754
                     /locus_tag="VNU03_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74096.1"
                     /translation="MPVLEVSGLEVRYGSVTAVKGISLEVAEGEVVAIIGPNGAGKTS
                     TLRAITGLVPTAGGRVVFRGTDVSRWRAHRIVTLGLGHAPEGRRLFPQMTVMENLRMG
                     AYRRRGPAEIGRTLAQVEELFPRLAERRAQLAGTLSGGEQQMLAIGRALMAEPRLLVL
                     DEPSFGLAPMIVREIGRIVRSINRERGVSVLLVEQNARMALGIAGRAYVMETGRVALS
                     GPSATLAESPHVKAAYLGG"
     gene            4757..5692
                     /locus_tag="VNU03_06270"
     CDS             4757..5692
                     /locus_tag="VNU03_06270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP74097.1"
                     /translation="MSTVKFGIAVGAYSAGVPSGRELADFARTAEAVGFDSVQVGDHI
                     QWHAPIIESTVLLATFAAVTTRVRIASDVVILPLRDPVLIAKTVASLDVLSGGRMIFG
                     VGVGGDHPGEYAAMRVPLAERGARANESLEIIRGLWTNERFSYAGRHYAVQDVGIAPR
                     PLQPLLPIWVGGTSDAALRRAARYGDGWICAFASERKFARLVEALRGFLAEAKRPADG
                     FTLGTFLFANVGPDPAQALAEGAEYVRRVYQLDGAAIVERFGAAGPVEHCVERVRALV
                     AAGAQYIVLSPVCGYRDWPRQLEAFGEVIARVGRG"
     gene            5695..>6489
                     /locus_tag="VNU03_06275"
     CDS             5695..>6489
                     /locus_tag="VNU03_06275"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWP74098.1"
                     /translation="MSAETVVVEHHGPVLRVRMNRPDKLNAQNPTLITELDTAFAAGA
                     ADADVRVIVLSGQGRAFSAGHDLEYPGYDQRLYTDQSRLEMERGLFIDNLLRLRNLPK
                     PTIAQVHGHCVAAGLMLACMCDLIVCDETARFANPVVAMAMPGVQLLIEPWEIGARKA
                     KELLFTGEALDAAEAHRLGLVNRVVAAGRLEDEVTALAARIAAMPPFAVQTVKRSIND
                     ALDFMGQGRSWEHHFPLHVAGKRSAEFRQWWQAVEQQGGGFRPGGLR"
BASE COUNT          920 a         2181 c         2299 g         1089 t
ORIGIN      
        1 gagcggatcc agacctggca tcgtgaggtc ggcgccgact acttcctgat ccgcttccgc
       61 cacccaggcg gccccgcaca ctcgcaggtg ctggaagccc tggagctctt tggccgcgag
      121 atcatccccc ggttccgctg atccgcaccc cgatccctca ggaggattgc tcatgcgcat
      181 gcaccgccgc atcgcgacgc tcgccactct cgccttcgcg gccgcgctct gtggcggcgg
      241 cgcggtccag gcggaggaca agaccctgac catcggtctg ctgggcccgc tgtctggcgg
      301 ggcggcgtcc tacggcgtcg agctgatgcg cggcgctgaa ctgcgggtgg aggagatcaa
      361 caagggcggc gggctcaagg tcggtgcgga cgtctaccgc atcaagcttg tgtcgtacga
      421 ccacaaggcg caggcggcgg acacggccac cgcgaccaat aagctcatct ttcaggacaa
      481 ggtgaagtac atcatcggca acgcggtggg cgccacctgc aacgccgcgc agacgatcac
      541 cgagcccaac aaggtgatgt ttgccttcgt atgctggggc accaacaacc tggccccaga
      601 gaagccgtac tcgttccggt cgatgctgag tcagtgggag ctggccgagc cgttctaccg
      661 ctgggtgaag gagaaccacc ccacgatcaa gcgggtggcg gccatctcac ccaatgacac
      721 gagcggcaag gacaccaaca ctgccgtagt gaaggcactc aaggcgctcg ggttcgaggt
      781 cgcggcggac gagtactacg aacgcgggac caaggacttc taccccgtgc tgacgaagat
      841 cctggcccag aagcccgaca tgatcgacgt ggcggccgcg ccgccgggcg aggccggcct
      901 gatcctcaag caggcgatgg agctcggctt caagggtgcc aagggctgga ccgcgggcat
      961 caaccccttc acgatcatca gcgtcgccgg gcccgaggcg gccgaaggcg tgtggagccc
     1021 cgcgaacatc aacgtcaagg gcgaccacgt gagcccggcc gtccggaagt tcggcgagac
     1081 gtacgagaag cgctacggcg aggtgcccgg cgccatcgcg gtggccaact acgcggcctt
     1141 cgacgtgttc acgcaggcca tgcagaaggc gggcagcatc gacaccgacc gggtgctggc
     1201 cgtcatcacg aaggagcagt tccagacggt ctgggggccg ctggccctgg gcggcaagga
     1261 gacgtacggc atcgaccggc agttcctcta tccgctggtc atcagcgaag tgcgacaggg
     1321 caaggtggtc gatatcgccc aggtgctgcc ggccgcattg aagaagaaat agggatccga
     1381 ccctgccggc ggggacgggg ctccgtccct ccccgctggc cgcactgccc atgcccatcg
     1441 agctgctgat ccaggcgctg ctgaacggct tcggcctggc cgtggtctat atcctggtcg
     1501 cgctgggcct gacgctcatt ttctcgattc tggaggtcat caacttcgcc cacggcgagt
     1561 tctacatgct gggcgggttc gcggcctact atctctccgc ggtgggcggg ctgaactacg
     1621 tcgccaccct cgtgctggct atgctcctgg tggggctggc gggcgtggtg gccgagcggc
     1681 tggtattccg tcacttgcgc ggcaagaccc tcaacgcgtt catcgtgtcg ctggggctcc
     1741 tgtgggtgct gcaggccacc gcccagctct cgttcggcgt gctcgacaag tcggtgccgt
     1801 cggcggtatc cggtattgtg cgcctggggg gcgtcatcgt gtcccgcgag cggatgttcg
     1861 tcatcctcac cgccgtcgcg ttgatcgtcg ggctccacct gtttctcaag ttcacccgca
     1921 ccggacaggc catgcgggcg gtggcgcagg acgcggaggc cgctgcgttg cagggcgtaa
     1981 acatcgagct gacctccgcg ctggggttcg gcatcggctg cgcgctggcc ggcgcggccg
     2041 gagccctgct cgcccccatc ttcgcggtga gcccgaccat gggcgcgctt cccgtggtca
     2101 aggccttcat catcatcatc gtgggcggga tgggcagcct acccggcgcc gtcctcggcg
     2161 gtctgctcct gggcgcggtg gagggcgtgg gcaccctgtt catgagcagc gccgcggtga
     2221 acatcctggg cttcctcatg gtgatcgcca tcctcctgct gcgaccgcga gggctgctcg
     2281 gtgccgcctg agccccggcg ggtcgacggc tggatcggct tcggcgtgct gctcgtcgcg
     2341 ggcgtctcgg tacccctgct cacgcgcgac tcctaccatc tcgacgtggc aaccgtgatc
     2401 ctgcgtaacc tgctgttgac catgagcctg cgctacgtca tgagtaccgg ccagctcaac
     2461 atggcccacg tgtcattcat ggggatcgcg gcctacgcct ccgccctcct cgtcacgcgg
     2521 gcgggctggt cgttctgggc ggccctggcc gtcgccccac tgctcgcggc gctggccgcg
     2581 gtcccgatgg ggcgcgtggc cctacgggtc agcggaccgt actactttct catcagcttc
     2641 gccttcctcg aggtcctgcg cctcgtcttc aacaacttct tcgtcgacta cctgggcggt
     2701 ccgtcggggc tggtcgagat cccgccgccc catccactgc tcctgacggg gggcgcggtg
     2761 accttcatcg gcaagctcgc ccagtactgg ctgatgctgg tgctgttcct ggcgagcgcg
     2821 gcggtgctca tccgcctcga gtgctcccgc gtcgggctgg tcaacggcgc catccgccag
     2881 ggcgagctgc tggccgagac gctgggcgtg aacgtccccc ggtacaagct gggggccttc
     2941 gtgctcgggg ccttcttcgc gggggtggcc ggcgtcttct ttgcgcactc gcagcgcgtg
     3001 ctgcactcga gtgacttcgg gctggagccg atggtgctgc tggtcgtgtt caccgtcatc
     3061 ggcggcgccg gcagcgtgtg ggggccggtg gtcgggacgc tcgtgctggc cgtcgcctcg
     3121 gagctgctgc gcgagctgca tcactacgag cttctcgtgt acggcttagt gctgatggtt
     3181 gtgatgctgt gcttcccgga cgggttggtg gcgctgcccg ggcgggtggc ccgccgcttc
     3241 ggctgggggc gggcgaagga cggggcgtcc accctggggg ccggcgttgc tccttgaact
     3301 gcgggaggtc ggcaagcggt tcggcggtct gcaggctgtg gccgcgttct cgatgacggt
     3361 gagagccggg acgatccacg gcctgatcgg cccgaatggc gcgggcaaga gcacggtgtt
     3421 caacctgatt accggcgtgt tgccggtgac ctccggcgag atccacttca agggcgagcg
     3481 catctcgggg ttgcggccct cggagatctg ccggcgcggc atcgcgcgga cgttccaggc
     3541 cacgaccttc ttccgcgagt tcaccgtgct cgacaacgtc atggctgcct cccacctcgc
     3601 cgtggagcgc ggcttcgtca agaccctgct cgccaccgcc ggctaccggg cccgcgaggc
     3661 cgccgcgctg gcccgggccc aggatctgct ggacgccgtc cggcttcagg acgtggccgg
     3721 ccggcgcgcg gggacgctgc cgcaccggga ccagaaggcg ctgagcctgg ctctagtgct
     3781 cgccaccggt gccggcctcg tcatcctgga cgagccgctg gcggggctga cgacgcagga
     3841 gggcgaggac gccatgcgta cgctgcaggc gctgcgcgcc gacggcaaaa cgatcctgct
     3901 cgtcgagcac gacatgcgcg cggtcatggg gttgtgcgac gtcatcactg tgctggacca
     3961 cggggtgcgg atcgccgagg gagtgccgcg cgacatccag gcgaacccgg cggtgatcga
     4021 agcctacctg ggcgcagagg atacccgtgc ttgaggtgag cggcctcgag gtgcgctacg
     4081 gcagcgtgac cgccgtgaag gggatctccc tcgaggtcgc cgagggtgag gtcgtggcga
     4141 tcatcgggcc caacggggcg ggcaaaacct cgaccctcag ggccatcacg ggcctggtgc
     4201 ccacggccgg cgggcgcgtg gtcttccggg gcaccgacgt ctcacgctgg agggcccacc
     4261 gcatcgtcac actcggcctg ggccacgcgc ccgagggtcg gcgcctgttc ccgcagatga
     4321 ccgtgatgga gaacctccgg atgggcgcct accggcggcg tgggcctgcc gagatcggcc
     4381 gcacgctcgc ccaggtcgag gagctcttcc cgcggctggc cgagcggcgg gcgcagctgg
     4441 cggggacgct gagcggcggc gagcagcaga tgctggcgat tggccgggca ctcatggcgg
     4501 agccgcggct gctggtgctc gatgagccct cgttcgggct ggcccccatg atcgtgcgcg
     4561 agatcgggcg gatcgtgcgc tcgatcaacc gcgagcgcgg ggtgtccgtt ctgctggtgg
     4621 agcagaacgc gcgcatggca ctggggatcg ccgggcgcgc ctatgtcatg gagacgggcc
     4681 gcgtagccct cagcggccca tcggccacgc tggccgagag cccgcacgtc aaagccgcct
     4741 acttgggagg gtaaggatga gcacggtcaa gttcggcatc gcggtcggag cgtactcggc
     4801 cggagtgccc tccggacgcg agttggccga cttcgcgcgt acagccgagg ccgttggctt
     4861 cgactcggtg caggtcggcg atcatatcca gtggcacgcc ccgatcatcg agtccaccgt
     4921 gctgctggcc actttcgcgg ccgtcaccac ccgagtccgg atcgccagcg acgtcgtcat
     4981 cctgcccctg cgcgacccgg tgctgatcgc caagacggtg gccagcctcg acgtgctatc
     5041 cggcggccgc atgattttcg gcgtcggtgt gggtggcgac catcccggtg agtacgccgc
     5101 catgcgggtg cccctggccg agcgcggcgc gcgggccaac gagagtctcg agatcatccg
     5161 tggcctgtgg accaacgaac gtttcagcta cgccgggcgg cactacgcgg tgcaggacgt
     5221 cggcatcgcg ccccggccgc tgcagccgtt gctcccgatc tgggtgggcg gcacctcgga
     5281 cgcagcgctg cgccgcgcgg cccgctacgg cgacggctgg atctgcgcct tcgcgagcga
     5341 gcgcaagttc gcgcggctgg tcgaggcgct gcggggattc ctggccgagg cgaagcgccc
     5401 ggccgatggc ttcacgctcg gcaccttcct gttcgccaac gtaggcccgg atcccgccca
     5461 ggccctggcg gagggcgccg agtacgtgcg ccgagtctat cagctggacg gggccgccat
     5521 cgtggagcgc ttcggggccg caggtcccgt cgagcattgc gtcgagcggg ttcgcgccct
     5581 ggtggccgcg ggggcgcagt acatcgtgct gtcgcccgtg tgtggctacc gcgactggcc
     5641 ccgtcaactc gaggctttcg gcgaggtcat cgcacgcgtg ggacggggct gagcgtgtcc
     5701 gccgagacgg tcgttgtcga gcaccacggc ccggtgctac gcgtgcgcat gaatcgtccg
     5761 gataagctca acgcgcagaa cccgaccctc atcacggagc tggacaccgc gttcgccgca
     5821 ggcgccgccg acgccgacgt gcgcgtcatc gtgctgagcg gtcagggccg cgccttctcc
     5881 gcgggtcacg acctcgagta tcccggctac gatcagcgac tctatactga ccagagccgg
     5941 ctggagatgg agcgcggcct gttcatcgac aatctcttgc gtctacgcaa cctgccgaag
     6001 ccgacgatcg ctcaggtgca cggacactgc gtggcggctg gcctcatgct cgcctgcatg
     6061 tgtgacctga tcgtgtgcga cgaaacggcc cgcttcgcca accccgtagt ggccatggcc
     6121 atgcccggcg tgcagctact gatcgagccc tgggagatcg gcgcccgcaa ggccaaggag
     6181 ctcttgttca cgggggaggc gctcgacgcg gctgaggcgc atcgcctcgg gctcgtgaac
     6241 cgggtcgtgg cggccggtcg cctcgaggac gaggtcacgg cgctggccgc gcgcatcgcc
     6301 gccatgccgc ccttcgcggt gcagacagtg aagcggtcaa tcaacgacgc gctcgacttc
     6361 atgggtcagg ggcgctcctg ggagcatcac ttcccgctcc atgtcgcggg caaacgcagc
     6421 gcagagttcc ggcaatggtg gcaggctgtc gagcagcagg gcggcgggtt ccggcccgga
     6481 ggcctccgg
//
LOCUS       DATMCV010000268         2296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_281918, whole genome shotgun sequence.
ACCESSION   DATMCV010000268 DATMCV010000000
VERSION     DATMCV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281918"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VNU03_06280"
     CDS             complement(<1..814)
                     /locus_tag="VNU03_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWP74099.1"
                     /translation="MSERPPAPDQAARDLIRERLDVNLLVEAGAGSGKTECLAQRMAM
                     GVLGGRYQVQQMAAVTFTRKAAAELRARFQLTLESALAAESDPVRRERALHALRHLER
                     LFAGTIHAFCAHLLRERPVEAGVAPGFSELDEVAEVDQRRWAWRDYLDRQRGEGAAAL
                     RELLDAGVRPVDLDRAFEKVCNYPEVAFPAGDAPRPDPARARAALEGFWAALTALLPD
                     GGPREATCSVQRRMREFTRRLRVASLDDPRVIAELAAEWESEPRVVQKWWPRG"
     gene            complement(807..>2296)
                     /locus_tag="VNU03_06285"
     CDS             complement(807..>2296)
                     /locus_tag="VNU03_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWP74100.1"
                     /translation="TRTFVIALEEGGVFPALVEDPVLLDAERKAIDPSLRTSEDRAGE
                     ALYRIVSRLGGLGGHVCVSYSGRDLRQARATFPSWVLLQAVRVAKPAGVWTYGRLMEE
                     LGEPVSAVPAEPGQALSDAGWWLAGLRRADASALPAVREGFPALAQGAAAEAARDSDE
                     FTVYDGLVPEAGPRLDPRVSGAAVSPTSLEELAKCPFRYFLQRGLGLGSVDDTEPNPD
                     VWLDPMTRGTILHDLFAAIMREIRDRKETPDPARHGARLRELGLAALARHRALVPPPS
                     DGVYEREERELLSDLALFLKFEAEDSARRRPLGFEVAFGGATEGEALGRREPVTIDLG
                     DGLRFELRGRIDRLDRLADGTYEAVDYKTGGAFLPGGLDAVFAGGRQLQHALYALAAV
                     ELLRETDGLARVVGSYYFPTTRGRRERQVRPVSTQAQATAVLRDLFDLLAAGAFVHTP
                     NQDEDCRFCEFDRACGPRAAERARAKIERGDNDPLEAYRRLSAHE"
BASE COUNT          301 a          885 c          833 g          277 t
ORIGIN      
        1 tgccgcgcgg ccaccacttc tggacgaccc ggggctcgct ctcccactcg gcggccagct
       61 cggcgatcac gcgcgggtcg tcgagggagg ccacccgcag gcgccgggtg aactcgcgca
      121 tgcgcctctg aacgctgcac gtggcctcgc ggggcccgcc gtcgggcagc agggcggtga
      181 gcgcggccca gaagccttcc agcgcggccc gcgcccgggc gggatcgggt cgcggcgcgt
      241 cgccggccgg gaacgccacc tcggggtagt tgcacacctt ctcgaacgcg cgatcgagat
      301 cgaccggccg cacgccggcg tcgagcagct cgcgcagggc cgcggcgccc tcgccgcgct
      361 ggcgatcgag gtagtcgcgc caggcccatc gccgctggtc cacctcggcc acctcgtcca
      421 gctcgctgaa gcccggcgcc acgccggcct ccacgggccg ctcccgcagc aggtgggcgc
      481 agaacgcgtg aatggttccc gcgaagagcc gctccaggtg ccgcagcgca tgcagggcgc
      541 gctcccgccg gaccggatcc gattcggccg cgagcgcgct ctccagcgtg agctgaaagc
      601 gcgcgcgcag ctccgccgcg gccttccggg tgaaggtgac ggcggccatc tgctggacct
      661 ggtagcggcc cccgaggact cccatcgcca tccgctgggc cagacactcg gtcttgcccg
      721 agccggcgcc cgcctcgacc agcaggttga cgtcgaggcg ctcgcggatc aggtcccggg
      781 cggcctggtc gggcgcgggg ggccgttcac tcatgcgcgc tcaatctccg gtacgcctcg
      841 agcgggtcgt tgtcgccacg ctcgatcttc gcccgcgccc gctcggcggc gcgggggccg
      901 cacgcccggt cgaattcaca gaagcggcag tcctcgtcct ggttcggcgt gtggacgaag
      961 gcgcccgcgg cgagcagatc gaagaggtcg cgcagcaccg cggtcgcctg cgcctgggtc
     1021 gagaccgggc ggacctgtcg ctcgcgccgg ccccgcgtgg tggggaagta gtacgagccc
     1081 accacccggg caagcccgtc cgtctcgcgc agcagctcga cggcggccag cgcgtacagg
     1141 gcgtgctgga gctggcgccc gcccgcgaac accgcgtcga gtccgccggg caggaacgcg
     1201 ccgccggtct tgtagtccac cgcctcgtag gtgccgtcgg ccagccggtc gagccggtcg
     1261 atccggccgc gcagctcgaa gcggaggccg tcgcccagat cgatcgtcac cggctcacgc
     1321 cggccgagcg cctcgccctc ggtggcgccg ccgaacgcca cctcgaagcc gagcggccgg
     1381 cgccgggcgg agtcctccgc ctcgaacttg agaaagagcg ccagatcgct cagcagctcg
     1441 cgctcctccc gctcgtagac gccgtccgac ggcggcggca ccagcgcgcg atggcgggcc
     1501 agggccgcca gccccagctc gcgcaggcga gccccgtggc gcgccgggtc cggcgtctcc
     1561 ttccggtccc tgatctctcg catgatcgcg gcgaagaggt cgtgcaggat cgtcccccgg
     1621 gtcatcgggt cgagccacac gtcgggattg ggctccgtgt cgtctaccga gccgagcccg
     1681 aggccgcgct ggaggaagta gcggaacggg cacttggcca gctcctcgag actggtgggc
     1741 gagacggcgg ctcccgacac gcgcggatcg aggcgcggcc ccgcttccgg cacgagcccg
     1801 tcgtagacgg tgaactcgtc ggagtcacgc gcggcctcgg ccgccgcgcc ctgcgcgagg
     1861 gcggggaagc cctcgcgcac cgcgggcagg gccgaggcgt cggcccggcg caggcccgcg
     1921 agccaccatc cggcgtcgct cagcgcctgg cccggctccg ccggcaccgc ggagacgggc
     1981 tcgcccagtt cttccatcag ccggccgtag gtccagacgc ccgcgggctt cgccacccgc
     2041 accgcctgca gcagcaccca cgacggaaag gtggcgcgcg cctgccgcag gtcgcggccc
     2101 gagtagctca cgcagacgtg cccgccgaga ccgcccagcc gcgagacgat gcggtagagg
     2161 gcctcgcccg cgcgatcctc cgacgtgcgc agggacgggt cgatggcctt gcgctcggcg
     2221 tccagcagca ccggatcctc caccagcgcg gggaagacgc cgccttcctc cagcgcgatg
     2281 acgaaggtgc gggtcc
//
LOCUS       DATMCV010000269         2501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_677188, whole genome shotgun sequence.
ACCESSION   DATMCV010000269 DATMCV010000000
VERSION     DATMCV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677188"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VNU03_06290"
     CDS             complement(<1..737)
                     /locus_tag="VNU03_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74101.1"
                     /translation="MSTDAAAAAATARSAPGRRRRGGRAAGALIVAAVAMVAALAPAL
                     PLADPNRTQPSGRLAPPLSPDHPLGSDHLGRDLLSRVVWGARVSLVVGVLATLLAMLA
                     GTAVGLVTGFYGGLTDNLAMRGIDLIMAFPYILLAMLLVAALGPGLVNAMLAVAVVNV
                     SFYARGVRAAVLLLREQEFVEAARAVGSTEGRILRRHILPGIAGPILVFVSMNIGVMI
                     TESAGLSFIGLGAQPPTADWGTMLADGR"
     gene            complement(727..1680)
                     /locus_tag="VNU03_06295"
     CDS             complement(727..1680)
                     /locus_tag="VNU03_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74102.1"
                     /translation="MRRYLTHRLFSALLVLVGVSFVVFLALHLAPGDPAQVLLGPMAT
                     PSELTNLRTQLGLDRPMIVQYGRWAGRAVQGDLGRSIMLRRPVLPELARRFQATLILA
                     GGALVLAFPTGILLGVLAATRPGSSIDRLSRIVAMVGISMPAFWVGLLLIIGFSVKLG
                     WLPGTGMYSPAGDGGARDLLVHLILPAVTLSLVPLAIVSRLTRSSMLDVIAQDYVRTA
                     RAKGASETRVIGWHAFRNTLVALVTVLGLQVGYLLAGAVYVETVFAWPGIGFMMVNAI
                     LTRDFPLVQGGVLLVAATYVVINLATDLFYAVVDPRVRHVH"
     gene            1837..>2501
                     /locus_tag="VNU03_06300"
     CDS             1837..>2501
                     /locus_tag="VNU03_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HWP74103.1"
                     /translation="MAIEAKSELRADVHSIEPIPESDKDSTGPQQMWIWAGANIAPIN
                     WALGALGIVLKLGLWETIAVIVLGNLVGCAIFSAFTVMGHQTGVNQMVLSRSAFGRRG
                     AYLPSALMFLMTLGWIGVNTYFPVKIAVAILGQFGIPDTWLTNFVVVTVVMVIQVLIG
                     IYGFYAIRTFEKYTVPVTVVIMALMSVLAWTRPGVVNWGLTSSLPPAAHFAMLTLLTT
                     AIGV"
BASE COUNT          413 a          887 c          898 g          303 t
ORIGIN      
        1 cggccgtcgg ccagcatggt gccccagtcc gcggtgggcg gctgggcgcc gaggccgatg
       61 aaggagaggc ccgcgctctc cgtgatcatg acgccgatgt tcatcgacac gaacaccagg
      121 atgggccccg cgatgccggg caggatgtgc cggcgcagga tgcgcccctc ggtggagccg
      181 actgcgcgcg ccgcctccac gaactcctgc tcgcgcagca gcagcaccgc cgcgcgcacc
      241 ccgcgggcgt agaacgacac gttcaccacc gccaccgcga gcatcgcgtt caccagcccg
      301 ggcccgagcg cggccaccag gagcatcgcg agcaggatgt agggaaaggc catgatgagg
      361 tcgatcccgc gcatcgccag gttgtcggtg aggccgccgt agaagccggt caccaggccg
      421 accgcggtcc ccgcgagcat ggccaggagc gtggcgagca cgcccaccac cagggagacg
      481 cgcgcgcccc acaccacgcg gctcaggagg tcgcggccca ggtggtccga gccgagaggg
      541 tgatccgggc tgagcggcgg ggccaggcgc ccgctcggct gcgtgcggtt gggatcggcg
      601 agcggcaggg ccggagccag cgccgcgacc atggccaccg cggccacgat cagggccccg
      661 gcggcccgcc cgccccggcg ccttcgcccg ggcgccgacc gcgcggtcgc ggccgccgca
      721 gccgcgtcag tggacatggc gcacgcgcgg atccaccacc gcgtagaaga gatcggtggc
      781 caggttgatc accacgtagg tggccgcgac cagcagcacg cccccctgca cgagcgggaa
      841 gtcgcgggtc aggatggcgt tgaccatcat gaagccgatg cccggccacg cgaacaccgt
      901 ctccacgtag accgcgcccg cgagcaggta gcccacctgc aggccgagca cggtgacgag
      961 ggcgacgagc gtgttgcgga aggcgtgcca gccgatcacc cgcgtctcgg aggcgccctt
     1021 ggcgcgcgcg gtgcgcacgt agtcctgcgc gatcacgtcg agcatgctcg agcgggtgag
     1081 ccgcgacacg atggcgagcg gcaccagcga cagggtgacc gcaggcagga tcaggtgcac
     1141 gagcagatcc cgcgcgccgc cgtcgcccgc cggcgagtac atgccggtgc cgggcagcca
     1201 gccgagcttc accgagaagc cgatgatcag caggagcccg acccagaacg cgggcatgct
     1261 gatccccacc atcgccacga tccgggagag ccggtcgatc gacgatccgg gccgcgtggc
     1321 cgccagcacc ccgagcagga tcccggtcgg gaaggccagc accagcgcgc cgccggccag
     1381 gatgagcgtg gcctggaagc gccgcgccag ctccggcagc accggccggc gcagcatgat
     1441 cgagcgcccg aggtccccct gcaccgcgcg cccggcccag cgcccgtact gcacgatcat
     1501 gggccggtcg aggccgagct gggtccggag gttcgtgagc tcgctcggag tcgccatcgg
     1561 gccgagcagg acctgggccg gatcgccggg cgccaggtgc agcgcgagga acaccacgaa
     1621 ggagacgccg accaggacga ggagcgcgga gaagaggcgg tgcgtcaggt agcgtcgcat
     1681 gcggtcggcg cgagtatagg aggggccggc ccgcgagggc aaggcgactc cgtcttgaca
     1741 cgagcggccc gaggggcgca gtatcgcgcc ggccgcgacg gcgtcgtccg agcccagcat
     1801 tgcccagtca caccgacacg ggtaaggggg ggcaccatgg cgatcgaagc caagagcgag
     1861 ctcagggccg acgttcacag catcgaaccc attccggagt ccgacaagga ctccaccggc
     1921 ccgcagcaga tgtggatctg ggccggcgcc aacatcgccc cgatcaactg ggcgctcggc
     1981 gcgctcggca tcgtgctcaa gctcggcctc tgggagacca tcgcggtcat cgtgctgggc
     2041 aacctggtgg gctgcgcgat cttctccgcc ttcacggtga tgggccacca gaccggcgtg
     2101 aaccagatgg tcctgagccg ctccgccttc ggccggcgcg gcgcctacct gccgagcgcg
     2161 ctgatgttcc tcatgaccct cggctggatc ggggtcaaca cctacttccc ggtgaagatc
     2221 gcggtcgcga tcctcggcca gttcgggatc cccgacacgt ggctgaccaa cttcgtggtg
     2281 gtgacggtgg tgatggtgat ccaggtgctg atcggcatct acgggttcta cgcgatccgc
     2341 accttcgaga agtacaccgt cccggtcacg gtggtgatca tggccctgat gagcgtgctg
     2401 gcctggacgc gcccgggggt cgtcaactgg ggcctcacca gctcgctccc gcccgcggcc
     2461 cacttcgcga tgctgaccct gctcacgacc gcgatcgggg t
//
LOCUS       DATMCV010000270         2210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_948674, whole genome shotgun sequence.
ACCESSION   DATMCV010000270 DATMCV010000000
VERSION     DATMCV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948674"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VNU03_06305"
     CDS             complement(<1..533)
                     /locus_tag="VNU03_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWP74104.1"
                     /translation="MTARKPLLDALVAMLTPRDVVVSCLGANARYLPHMTVPCPVFAL
                     CDSMGAAIPLGLGMALQRPDRHVIALEGDGSLLMSPNVLATVAAAAPPNLTMLLWVNG
                     RYESSGGQALPAVTVDWAALARAAGLRHVATVGDVAGLSSAFTAARSAGGPAAIVVEI
                     AFDPAEQIPPYSERPDE"
     gene            complement(530..1024)
                     /locus_tag="VNU03_06310"
     CDS             complement(530..1024)
                     /locus_tag="VNU03_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWP74105.1"
                     /translation="MTGIERIVAGLEAAQVSIVASVPDTWIGRLMEGVRRSPRLRAVD
                     VAREEEAVAVACGANLAGGRGAVLIQNAGLLNCGGVLAGLVELYHLPCFFIVSLRGDH
                     RDPVYYHAPKGRVTEETLRAWRLPYARADRRGDLAVQVKQGVEFCVESRGPFVLLISG
                     EDLA"
     gene            complement(1021..2100)
                     /locus_tag="VNU03_06315"
     CDS             complement(1021..2100)
                     /locus_tag="VNU03_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74106.1"
                     /translation="MARAVVELAGCSREYGGVRAVAGLDLVVYEGELLSLLGPSGCGK
                     TTTLNLIAGFVLPTAGRVRIDGQDVTDRPAHQRGLGMVFQSYALFPHLSVFENVAFGL
                     RERRTPRAEIERRVRAALALVRLDTRADHRPAQLSGGMQQRVALARALVYEPRVLLLD
                     EPLAALDKKLREEMRSELREIQRAVGITTIFVTHDQAEALGLSDRIAVMHAGRVEQLG
                     SPREIYERPATRFVAEFIGASTVLRGHGAAADRVTLAGGEALRVLGLPGIRAGEAIEL
                     AIRPERIRLAGGAGENVMDGRVTGVVYQGVQTELTVEIGAGQRLLVFVAEPAPTALAV
                     GQALRLHLPADAFMRLGPAGAEAGR"
BASE COUNT          314 a          846 c          797 g          253 t
ORIGIN      
        1 atctcgtcgg gccgctcgga gtagggtgga atctgctccg cggggtcgaa cgcgatctcg
       61 accacgatgg cggcggggcc gcccgcgctg cgcgcggccg tgaaggcgct gctcagcccg
      121 gccacgtcgc ccaccgtggc cacgtgccgc aggcccgccg cgcgcgctag cgcggcccag
      181 tccaccgtca ccgcgggcag cgcctggcca ccggaggact cgtagcggcc gttgacccag
      241 agcagcatgg tgagattggg cggtgccgcc gcggccaccg tggccagcac gttcgggctc
      301 atcagcagcg agccgtcgcc ctccagcgcg atcacgtggc ggtcgggccg ctggagcgcc
      361 atgccgaggc ccagcgggat ggccgcgccc atcgaatcgc agagggcgaa gaccgggcac
      421 ggcaccgtca tgtgcggcag gtagcgggcg ttggcgccga ggcaggagac gaccacgtcg
      481 cgtggggtca gcatcgcgac cagcgcgtcc agcagcggct tgcgggcggt cacgccaggt
      541 cctcgccgct gatcagcagc acgaagggcc cgcgcgactc cacgcagaac tcgacgccct
      601 gcttcacctg gaccgcgagg tcgccccggc gatcggcccg cgcgtaaggc aggcgccagg
      661 cccgcagcgt ctcctcggtc acgcggccct tgggcgcgtg gtaatagacg gggtcgcggt
      721 ggtcgccgcg cagcgacacg atgaagaagc acggcaggtg gtagagctcg accagcccgg
      781 cgagcacgcc gccgcagttg agcagccccg cgttctggat caggaccgcg ccgcggccac
      841 cggccaggtt ggccccgcac gcgaccgcca ccgcctcctc ctcccgcgcc acgtcgactg
      901 cgcgcagccg cggtgaccgg cgcacgcctt ccatcagccg cccgatccac gtgtccggca
      961 ccgaggccac gatcgagacc tgggccgcct cgaggcccgc gacgatccgc tcgatcccgg
     1021 tcaccggccc gcctccgcgc cagcgggacc gaggcgcatg aacgcgtccg cgggcagatg
     1081 caggcgcagc gcctgcccca ccgcgagcgc ggtgggcgcc ggctcggcca cgaagacgag
     1141 cagccgctgc ccggccccga tctcgacggt cagctcggtc tggacgccct ggtagaccac
     1201 gccggtgacg cgcccgtcca tcacgttctc ccccgcgccg ccggcgagcc ggatgcgctc
     1261 cggccggatg gccagctcga tcgcctcccc cgcccggatc ccgggcaggc ccagcacccg
     1321 cagcgcctcg ccgccggcca gcgtcacccg atccgcggcg gcgccgtggc cgcgcagcac
     1381 ggtggaggcg ccgatgaact cggccacgaa ccgggtcgcg ggccgctcgt agatctcgcg
     1441 cgggctgccg agctgctcga cgcgcccggc gtgcatcacc gcgatgcggt cggacaggcc
     1501 gagcgcctcg gcctggtcgt gggtgacgaa gatggtggtg atgccgaccg cgcgctggat
     1561 ctcccgcagc tcgctccgca tctcctcgcg gagcttcttg tcgagcgcgg cgagcggctc
     1621 gtccagcagc agcacccgcg gctcgtagac gagcgcccgg gccagggcta cccgctgctg
     1681 catgccgccc gagagctggg ccgggcggtg gtcggcacgg gtgtcgagtc gcaccagggc
     1741 gagggccgcg cgcacccgcc gctcgatctc cgcccgcggc gttcgccgct cgcgcagccc
     1801 gaaggccacg ttctcgaaca ccgagaggtg cgggaagagc gcgtacgact ggaacaccat
     1861 cccgagcccc cgctggtggg cggggcggtc ggtcacgtcc tggccgtcga tccgcacccg
     1921 gcccgcggtc ggcagcacga agcccgcgat caggttgagg gtggtggtct tgccgcagcc
     1981 ggacgggccg aggagcgaga gcagctcgcc ctcgtacacg accaggtcga ggccggcgac
     2041 cgcacgcact cccccgtatt cccggctgca gccggccagc tcgaccaccg cgcgggccat
     2101 cagcgccgcc tcaccgatgg tggaggaaca cgccgcccag gtccaggtac cggtcggcga
     2161 gccagacgat caggaaggtg cccacgatga acagggtcga gagcgcggcg
//
LOCUS       DATMCV010000271         2828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_880003, whole genome shotgun sequence.
ACCESSION   DATMCV010000271 DATMCV010000000
VERSION     DATMCV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880003"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VNU03_06320"
     CDS             <1..632
                     /locus_tag="VNU03_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HWP74107.1"
                     /translation="RSGAVIYEIRTYRVKVGSLPEVEKRFGEAYEQRKKISPLAAFWH
                     TEIGPLNEIIHVWPYENISERNRLRADAVKAGNWPPKIAEFIETMQSEIVVPFAFLPD
                     LKPGKMGPYFEMRYYAMKAGTLPDLIKRWESKIEERVKMSPIALAGHVEHGEANRFIH
                     IWAYKSLDERAAVRNKAREMGVWPPPGGSDTLLTMANKIMMPAAFSPVQ"
     gene            complement(650..1417)
                     /locus_tag="VNU03_06325"
     CDS             complement(650..1417)
                     /locus_tag="VNU03_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP74108.1"
                     /translation="MTRASLNGIDIDYQASGSGPAILLSHGYSATRRMWDAQHAVLGE
                     QHRVISWSMRGHGDTESPADPAQYSADLTVRDMHSLLGHLGVERAVIGGLSLGGYVSL
                     AFYLAHPEMVRALVICDSGPGYRSADARAAWNQRAQERAAALEARGLEALGKSREVRE
                     SASLHRSAQGLAHAARGMLAQEGSRVIDGLAGIAVPTLIIVGDQDQPFLAPCEYMAKK
                     IPGARLEVIRGAGHSSNLDQPEAFNRVLLDFLSGLPV"
     gene            1445..>2828
                     /gene="iaaH"
                     /locus_tag="VNU03_06330"
     CDS             1445..>2828
                     /gene="iaaH"
                     /locus_tag="VNU03_06330"
                     /inference="COORDINATES: protein motif:HMM:NF005688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indoleacetamide hydrolase"
                     /protein_id="HWP74109.1"
                     /translation="MHTIGWPGSWRWGWRWVTGLMLLAVPAGAAAQDLTELSASEAAA
                     RIRAGTLKSEDLVRALIDVIERKRDLNAFITFDRDRAIASARKADTLAARKSFAGPLH
                     GVPIVVKDNIHVAGLPNSAGTPALRDFVPTRNAPVAEKLIRAGAIVLGKANMHELAFG
                     VTSNNAAFGPARNAYDPSRIAGGSSGGTANAIAARMAAAGLGSDTGGSVRIPAALNGI
                     AGLRPTLGRYSQEGITPIAHTRDTAGPMAREVVDLVLLDTIITGARDRVAAPPLKGLR
                     LGVCRSLFFRSLDSETERLTEATIDRLQAAGVEIVEVEMPGLVDLNGKIGFPVALYEA
                     RVDLTAYLKRYRPALDLRGLAERIASPDVKGLFASAIVPGAKDAIPQKAYLDALAARP
                     LLQQLYAETFRKHNIVALVFPTTPLPAAPVGDDATTLLNGSQVPTFPTFIQNTDPGSN
                     AGIPGLSVPIG"
BASE COUNT          436 a         1026 c          986 g          380 t
ORIGIN      
        1 aaaggagtgg agccgtgatc tacgagatcc gcacgtaccg cgtgaaggtc ggcagtctcc
       61 ccgaggtgga gaagcgcttc ggcgaggcct acgagcagcg caagaagatc tcgcccctcg
      121 ccgcgttctg gcacaccgag atcgggccgc tcaacgagat catccacgtg tggccctacg
      181 agaacatcag cgagcgcaac cgcctccggg cggacgcggt caaggcgggc aactggccgc
      241 ccaagatcgc ggagttcatc gagaccatgc agtcggagat cgtggtgccg ttcgcgttcc
      301 tgcccgacct caagcccggc aagatgggcc cctacttcga gatgcgctac tacgcgatga
      361 aggcgggcac cctgccggac ctgatcaagc gctgggagtc gaagatcgag gagcgggtca
      421 agatgtcgcc gatcgcgctg gccggccacg tcgagcacgg cgaggccaac cgcttcatcc
      481 acatctgggc ctacaagagc ctggacgagc gggccgcggt ccggaacaag gcgcgcgaga
      541 tgggcgtgtg gccgcccccg ggcgggagcg acaccctgct cacgatggcc aacaagatca
      601 tgatgccggc cgccttctcg ccggtccagt aggccggccg ccggctaggt tagacgggga
      661 ggccgcttag gaagtcgagc agcacgcggt tgaaggcctc cggctggtcg aggttcgagg
      721 agtgtccggc gccccggatg acctcgagcc tcgccccggg gatcttcttg gccatgtact
      781 cgcacggggc caggaagggc tggtcctggt cgcccacgat gatcagcgtc ggcaccgcga
      841 tgccagcgag gccgtcgatc acccgcgagc cctcctgggc gagcattccg cgcgcggcgt
      901 gcgccagccc ctgggccgag cggtgcaggc tcgccgactc gcgcacctcg cggctcttgc
      961 cgagcgcctc gaggccgcgg gcctcgagcg cggcggcgcg ctcctgggct cgctggttcc
     1021 aggccgcgcg cgcgtccgcg ctgcggtacc cggggccgga gtcgcagatg acgagcgcgc
     1081 gcaccatctc gggatgcgcc aggtagaagg cgagcgagac gtagccgccc agggagagcc
     1141 cgccgatcac cgcgcgctcg acgcccaggt ggcccagcag cgagtgcatg tcgcgcaccg
     1201 tgaggtctgc tgagtactgg gccggatccg ccgggctctc ggtgtcgccg tggccgcgca
     1261 tgctccagga gatgacccgg tgctgctcgc ccagcaccgc gtgctgcgcg tcccacatgc
     1321 ggcgggtcgc cgagtagccg tggctcagca ggatggccgg gccggagccg ctcgcctggt
     1381 agtcgatatc gatgccgttg agactggctc gcgtcatcgc gcccgagtat gccagaatcg
     1441 cgccatgcac accatcggat ggccgggcag ctggcgatgg ggatggcggt gggtcacggg
     1501 cctcatgctg ctcgcggtgc cggcgggggc ggcggcccag gacctgaccg agctgtcggc
     1561 ctccgaggcg gcggcgcgca tccgggccgg cacgctcaag agcgaggacc tggtgcgggc
     1621 gctcatcgac gtgatcgagc ggaagcgcga cctcaacgcc ttcatcacct tcgatcgcga
     1681 tcgggcgatc gcctccgcgc gcaaggcgga caccctcgcg gcccgcaaga gcttcgcggg
     1741 gccgctgcac ggcgtgccga tcgtggtgaa ggacaacatc cacgtggccg gcctgccgaa
     1801 ctccgcgggc acccccgcgc tgcgcgactt cgtcccgacc cgcaacgccc cggtggccga
     1861 gaagctgatc cgggccgggg ccatcgtgct cggcaaggcc aacatgcacg agctggcctt
     1921 cggggtcacg agcaacaacg cggccttcgg gccggcgcgc aacgcctacg acccgagccg
     1981 catcgcgggc ggatcgtcgg gcggcaccgc caacgccatc gcggcccgca tggccgcggc
     2041 ggggctcggc agcgacaccg gtggctcggt gcgcatcccc gccgccctca acggcatcgc
     2101 ggggctccgc ccgacgctcg gccgctactc ccaggagggc atcactccga tcgcccacac
     2161 gcgggatacc gcggggccga tggcccgcga ggtcgtcgac ctggtgctgc tcgacaccat
     2221 catcaccggc gcgcgcgatc gcgtcgccgc cccgccgctc aagggcctcc gcctgggagt
     2281 ctgccggtcg ctcttcttcc ggagcctcga ctccgagacc gagcggctca ccgaggcgac
     2341 gatcgaccgg ctccaggccg cgggggtgga gatcgtcgag gtcgagatgc cgggcctcgt
     2401 cgacctcaac ggcaagatcg gcttcccggt cgccctctac gaggcccgcg tggacctcac
     2461 cgcgtacttg aagcgctacc gccccgccct cgacctgagg gggctcgccg agcgcatcgc
     2521 gagcccggac gtgaaggggc tcttcgccag cgcgatcgtt cccggggcca aggacgcgat
     2581 cccccagaag gcctacctgg acgcgctcgc cgcgcggccg ctgctccagc agctctacgc
     2641 ggagaccttc cgcaagcaca acatcgtcgc gctcgtgttc cccaccacgc cgctgcccgc
     2701 cgcgccggtc ggagacgacg ccacgacgct gctcaacggg tcccaggtcc cgaccttccc
     2761 taccttcatc cagaacaccg atccgggcag caacgcgggc atccccgggc tgagcgtgcc
     2821 gatcgggc
//
LOCUS       DATMCV010000272         3016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_547351, whole genome shotgun sequence.
ACCESSION   DATMCV010000272 DATMCV010000000
VERSION     DATMCV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547351"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2983
                     /locus_tag="VNU03_06335"
     CDS             <1..2983
                     /locus_tag="VNU03_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWP74110.1"
                     /translation="PDLTDPTVVVVTLYPGFAAEEVEQQVTVPIERALNNTPYVISRR
                     SRTIFGLSVVELTFEDGTNDFLGRQLVLERLQGAQLPDGVTPTLGPLSGGVSEFYRYV
                     VAGPGVETATLREIQDWVIGPRLLQVPGVADVATFGGLVRQYEVQVDPRALDKYHLSM
                     RQIADAVKSNNRNAGGALLRMGQQELPIRGSGLIQSPDDLEGIVLDAPRGVPIRMGDI
                     GRVRMGALPQTGIFAMDDEPVGSGGVEGIVLMRRGENPSEVLPRVREAVDELNATRLP
                     SGLRIVAIHDRSDLVANTLHTVSHVLIAGFTIVVIALLAFLLSARAALLTAVVIPLSL
                     LFALVCMYASGVSLSLLSIGALDFGIIVDGTIVMVERIIRGLENNPHGDPRETIIAAA
                     VEAQRPILFSMLVIIAAYIPLLTLQRVERRLFMPMALTVCYALLGSLLLALTLIPALA
                     TFLFRGHIRILRHPPLERLNDWYGRAIRSLVDRAGWVVGAAAVVVAGALGLGTTLGTE
                     FLPQLDEGVIWIRANLPAGIALGKSAELARQIRMLIRQSPEVRRVASQTGRNDSGTDP
                     FGPNRNELLVDLAPYDTWAGGRTKAGLVDELARRLTTTIPGAAFNFTQPIIDTATEIV
                     TGSSADLAVIISGSDLRELRRLAGQTLGILAPMRGAADTSIEQEADQPQLRIAIDRPR
                     VARYGINVGDVQDVIDLALGGAPIGGVFEGERRFDIVARFTPESRGSPGAIAELLIPT
                     RDGARVPLLQLADIRTSDGATIIARRENRRAITVRTNIRGRDQGGFVGEAQERFARGV
                     TLPPGYSVDWGGQFENFARAKARLAFIMPITLAVIFVLLFVTFGTALDAGLVLLTVPF
                     SMAGGVLALYLRGMNLNVSAAVGFISLFGVAVMSGVLYISDITRRRGRAGVPLRDAVV
                     AASRAQLRPVLLVIVVATLGMIPAMVAHGIGSDIQRPLATVVVGGLLSTLFLTPLALP
                     AVYVLAERFRPAPPDAGAAHR"
BASE COUNT          397 a         1084 c         1057 g          478 t
ORIGIN      
        1 tccggatctg accgacccga cggtggtcgt ggtcaccctc tatcccggct tcgcggccga
       61 ggaggtcgag cagcaggtca cggtcccgat cgagcgggcg ctcaacaaca cgccctacgt
      121 catctcgcgc cgctcgcgca cgatcttcgg gctctcggtc gtcgagctga ccttcgagga
      181 cggcaccaac gacttcctgg gccggcagct cgtcctggag cggctgcagg gcgcccagct
      241 cccggacggc gtcaccccca ccctcggccc gctgtcgggc ggcgtgagcg agttctaccg
      301 atacgtggtg gcggggcccg gggtcgagac cgcgaccctg cgggagatcc aggactgggt
      361 gatcggcccg cggctcctcc aggtgccggg agtggccgac gtcgccacgt tcggcggcct
      421 cgtgcgtcag tacgaggtgc aggtcgatcc ccgtgcgctc gacaagtatc acctgagcat
      481 gcggcagatt gcggacgcgg tgaagagtaa caaccggaac gcgggtgggg ccctgctccg
      541 gatggggcag caggagcttc ccatccgcgg ctccgggttg atccagtctc ccgacgacct
      601 cgaaggcatc gtgctcgacg cgccgcgggg cgtgccgatc cggatgggcg acatcggccg
      661 ggtgcggatg ggcgccctgc cccagaccgg gatcttcgcg atggacgacg agccggtcgg
      721 ctccggaggg gtcgagggca tcgtcctcat gcggcgcggc gagaacccga gcgaggtgct
      781 gccccgggtg cgcgaggcgg tggacgagct gaacgccacg cggctgccca gcggcctgcg
      841 gatcgtggcc atccacgacc gatcggacct ggtcgccaac acgctccaca ccgtgtcgca
      901 cgtgctgatc gcgggcttca cgatcgtggt gatcgccctg ctcgcgttcc tcctgagcgc
      961 gcgcgccgcc ctgctgaccg cggtcgtgat ccccctgtcg ctgctgttcg cgctcgtctg
     1021 catgtacgcg agcggcgtct cgctgagcct gctcagcatc ggcgccctcg acttcgggat
     1081 catcgtggac ggcacgatcg tgatggtcga gcgcatcatt agaggtctgg agaacaaccc
     1141 gcacggtgac ccgcgggaga ccatcatcgc cgccgcggtg gaagcccagc gacccatcct
     1201 cttctcgatg ctggtgatca tcgccgccta catcccgctc ctgaccctcc agcgggtgga
     1261 gcgccgcctg ttcatgccca tggcgctcac cgtctgctac gccctgctgg gctcgctgct
     1321 cctcgccctg accctgatcc cggcgctcgc gacctttctc ttccgaggcc acatccggat
     1381 ccttcgccac ccgccgctcg agcggctcaa cgactggtac ggccgggcaa tccggagcct
     1441 cgtggaccgg gccggctggg tggtgggcgc cgcggcggtg gtcgtcgccg gcgcgctcgg
     1501 cctcgggacc acgctcggca ccgagttcct gccgcagctc gacgagggag tgatctggat
     1561 ccgcgccaac ctgccggccg gcatcgcgct cggcaaatcg gccgagctgg cgcgccagat
     1621 ccggatgttg atccgccagt cgcccgaggt gcggcgagtg gcctcgcaga ccgggcggaa
     1681 cgactcgggg accgatccct tcggtccgaa ccggaacgag ctgctcgtgg acctcgcgcc
     1741 ctacgacacc tgggcggggg gacgtaccaa ggcggggctg gtggacgagc tggcgcgccg
     1801 gctcaccacc accatccccg gggcggcctt caacttcacc cagccgatca tcgacaccgc
     1861 gacggagatc gtgaccgggt cgtcggcgga cctggccgtg atcatcagcg gctcggatct
     1921 ccgggagctg cgccgcctcg cggggcagac gctcggaatc ctcgcgccga tgcgcggcgc
     1981 ggccgacacg tccatcgagc aggaggcgga tcagccgcag ctccggatcg cgatcgaccg
     2041 gccgcgcgtg gcccgctacg gcatcaacgt gggcgacgtg caggacgtca tcgacctggc
     2101 gctgggcggc gcgcccatcg gaggggtgtt cgagggggag cggcgcttcg acatcgtggc
     2161 gcggttcacc cccgaatcgc gcgggagccc cggggccatc gcggagctgc tgatcccgac
     2221 ccgcgacggc gcccgggtgc cgctgctcca gctcgccgac atccgcacct ccgacggcgc
     2281 caccatcatc gcgcgacggg aaaaccgccg ggccatcacc gtgcgcacca acatccgggg
     2341 gcgggaccag ggcggcttcg tgggagaggc ccaggagcgg ttcgcccggg gcgtcaccct
     2401 gcctcccggg tattcggtgg actggggcgg ccagttcgag aacttcgcgc gggccaaggc
     2461 ccgcctcgcc ttcatcatgc cgatcaccct cgcggtgatc ttcgtcctgc tcttcgtgac
     2521 cttcgggacc gcgctcgacg cggggctggt cctcctcacg gtgccgttct cgatggcggg
     2581 aggggtcctg gctctctacc ttcggggcat gaacctcaac gtctcggccg cggtgggctt
     2641 catctctctc ttcggggtcg cggtgatgag cggggtgctc tacatctccg acatcacgcg
     2701 gcggcgcggc cgggccgggg tgcctctgcg ggatgcggtg gtggcggcgt cgcgcgccca
     2761 gctgcggccg gtgctgctgg tgatcgtggt ggccacgctc ggcatgatcc cggccatggt
     2821 cgcgcacggc atcggctccg acatccagcg gccgctcgcc accgtcgtgg tcggcggcct
     2881 gctctcgacc ctcttcctga ccccgctcgc gctgcccgcg gtctatgtgc tggccgagcg
     2941 ctttcgaccg gctccgcccg acgcgggggc cgcgcatagg tagctgatcg acctggaacc
     3001 tagcgccggc gcggcg
//
LOCUS       DATMCV010000273         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_880077, whole genome shotgun sequence.
ACCESSION   DATMCV010000273 DATMCV010000000
VERSION     DATMCV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880077"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VNU03_06340"
     CDS             complement(<1..773)
                     /locus_tag="VNU03_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74111.1"
                     /translation="MTGWRSTTTAMLLLALGLLAAPGALLAQTPTPKYGGVLTLMQRE
                     ELPQGLSILETSTIATVWPASPCFNNLVYFDPAKPMETPDTIVGELAEKWSWQDGYRN
                     LVFFLRRDVKWHDGQPFTSRDVKFTFDLVREAPDAVGKLRLNPRKDWYANVEAIEAAD
                     PYTVVFRLKRPQPSILMMLASGQSPIYPAHVPLADQRSRCVGTGPFKFKEWRRGEYVE
                     YVKNPDYFVKGRPYLDGLKYVIISERGTRTAALQAGRLD"
     gene            complement(792..>2428)
                     /locus_tag="VNU03_06345"
     CDS             complement(792..>2428)
                     /locus_tag="VNU03_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWP74112.1"
                     /translation="ESLIAHGVEYIFGNPGTTESPILDALLDYPQLRYIVALHEGVAL
                     GAASYYAQATGRPAVVNVHVAPGLGNALGMLYNAFKARAPLVVTAGQQDTRLRLRGPV
                     LGHDLVAMAAPLTKWSVQVERADEFPLIMHRALKIASDPPAGPVFVALPIDVMEQETD
                     QAPLPPGRLHRASEPDPAGIEAAATLLLGSRRPVIVVGDDAAGATGELTTLAEQLGAA
                     VWCEGIRAHQVMPSAHPNFRLGLPFDAVAIRRALDDADVVVLVGGPFFEEVWYAPGSP
                     LPPGAAAIQVESSPERLAHNLPVTVGLVSHPRAALSALRAALERGAAAAFRSAAAARN
                     EALRALKAQDAQAQRARAAKRWDSAPISMPRFMAEIEAALPPDAIVVDESITAGPDLA
                     RTVQFERAGDYVGARGGGIGQALPGALGIKLARPDRPVVALSGDGSAMYSIQALWTAA
                     HHDLAVAFVILNNREYRILKHNMDTYRQRFGAKPDRGYPNMDLVSPDLGFVDIARGLG
                     VEGARVSTPGELRPALHRALGAGRPFLLDVAIEGRP"
BASE COUNT          325 a          843 c          920 g          340 t
ORIGIN      
        1 ctgtcgagcc gcccggcctg cagcgcggcg gtccgggtgc cgcgctcgct gatgatcacg
       61 tacttgagcc cgtcgaggta gggccgcccc ttcacgaagt agtccgggtt cttcacgtac
      121 tcgacgtact cgccccgccg ccactccttg aacttgaacg ggccggtgcc cacgcaacgg
      181 ctgcgctggt cggcgagcgg cacgtgggcc ggatagatcg gcgactggcc ggaggcgagc
      241 atcatgagga tcgagggctg cggccgcttg agccggaaca cgacggtgta gggatccgcc
      301 gcctcgatcg cctcgacgtt ggcgtaccaa tctttcctcg ggttgagtcg gagcttgccg
      361 accgcgtcgg gggcctcgcg caccaggtcg aaggtgaact tgacgtcgcg cgaggtgaac
      421 ggctggccgt cgtgccactt cacgtcccgg cgcaggaaga acaccaggtt gcgatagccg
      481 tcctgccacg accacttctc ggccagctcg cccacgatgg tgtcgggcgt ctccatgggc
      541 ttcgcgggat cgaagtagac caggttgttg aagcagggcg acgcgggcca gacggtggcg
      601 atcgtcgagg tctcgaggat cgagaggccc tgtgggagct cttcccgctg catgagggtg
      661 agcacgccgc cgtactttgg cgtcggcgtc tgggcgagga gcgcgcccgg ggccgcgaga
      721 aggcccagcg ccaggagcag catcgccgtc gtcgtcgatc gccacccggt cattcgggac
      781 tcctcgagcc gctaggggcg gccctcgatc gcgacgtcga gcaggaaggg gcgcccggcg
      841 ccgagcgccc ggtgaagcgc cgggcgtagc tcgccgggcg tgctcacacg cgcgccctcc
      901 acccccaggc cgcgcgcgat gtcgacgaag cccagatcgg gggacacgag atccatgttc
      961 gggtagcccc gatccggttt cgcgccgaag cgctggcggt aggtatccat gttgtgcttc
     1021 aggatgcggt actcgcggtt gttcaggatc acgaaggcga ccgcgaggtc gtgatgggcc
     1081 gcggtccaga gcgcctggat gctgtacatc gcggagccgt cgccggagag cgcgaccacc
     1141 gggcggtccg gccgggccag cttgatgccg agggcgcccg gcagcgcctg gccgatgccg
     1201 ccgccgcggg cgcccacgta gtcgcccgcg cgctcgaact ggacggtgcg ggcgaggtcg
     1261 gggccggcgg tgatcgactc gtcgaccacg atcgcgtccg gcggcagcgc ggcctcgatc
     1321 tcggccatga agcgcggcat cgagatcggc gcgctgtccc agcgcttcgc ggcgcgcgcc
     1381 cgctgcgcct gcgcgtcctg ggccttgagc gcgcggagcg cctcgttccg cgccgccgcg
     1441 gccgaccgga aggcggccgc ggcgccgcgc tccagcgccg cgcgcagggc cgagagggcc
     1501 gcgcggggat ggctcacgag gccgaccgtc accggcaggt tgtgggcgag gcgctccggg
     1561 gacgactcga cctggatcgc ggcggcgccg ggcgggagtg gggagccggg ggcgtaccag
     1621 acctcctcga agaacgggcc gccgaccagc accaccacgt ccgcgtcgtc gagggcccgg
     1681 cggatggcga ccgcgtcgaa gggcaggccg aggcggaagt tcgggtgcgc gctcggcatc
     1741 acctggtggg cccggatccc ttcgcaccag accgcggcgc cgagctgctc ggccagcgtg
     1801 gtcagctcgc cggtcgcgcc ggccgcgtcg tcgcccacga cgatgaccgg ccgccggctg
     1861 ccgagcagga gcgtggcggc cgcctcgatc ccggccgggt cgggctcgga cgcgcggtgc
     1921 agccggcccg ggggcagggg ggcctggtcg gtctcctgtt ccatgacgtc gatcggcagc
     1981 gcgacgaaca ccggccccgc cggtgggtcg ctggcgatct tcagcgcgcg gtgcatgatc
     2041 aaggggaact cgtcggcgcg ctcgacctgg acgctccact tggtgagcgg cgcggccatc
     2101 gccaccaggt cgtgcccgag caccgggccc cgcaggcgca ggcgggtgtc ctgctgtccc
     2161 gcggtcacca cgagcggcgc ccgggccttg aacgcgttgt agagcatgcc gagcgcgttg
     2221 ccgagccccg gcgccacgtg cacgttgacc accgcgggcc gcccggtcgc ctgcgcgtag
     2281 tagctggccg cgccgagggc caccccttcg tggagcgcca cgatgtaccg cagctgcggg
     2341 tagtccagca gcgcgtcgag gatggggctc tcggtggtgc cggggttgcc gaagatgtac
     2401 tccaccccgt gcgcgatcag gctctcca
//
LOCUS       DATMCV010000274         5494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_282232, whole genome shotgun sequence.
ACCESSION   DATMCV010000274 DATMCV010000000
VERSION     DATMCV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282232"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1966)
                     /locus_tag="VNU03_06350"
     CDS             complement(236..1966)
                     /locus_tag="VNU03_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74113.1"
                     /translation="MRIPDEQMDLFAKRLEAIGVGRRDFLKVVGAMAAFGGLGFATTA
                     QAAKPSKPGPNEKLAKEQAFRFGGGGWFQNDPASHDFNKDLYCSGVPALFAGLMVFNA
                     DFVSVPWMASKVEGNKDGSVWTFTIRKDSRWSDNSPVSARDFEWSWKRQLDPASAAPY
                     ASFLYDIKNGEAYNKKQITDASQVGVRAKDDWTLEVTLDGPRGYFPVLASYLAALPAH
                     RGAVEKFGDKWTEAGNIVSNGPFTLEAWEHNKQIVLKKNPHYFGAKDVHLSRVVIPIV
                     PVASGSLPYENNELDMTALQSGDLKRLQSDPRMGKDVFRYPFPGTWYLLPQVSKPPFD
                     NLKVRKAVAQAIDRENVVKVSQGFAIPAWSMIPPGFPGAIDDAGIKAIQRYDKKAALD
                     ALKGTPYEGGKNWPKITLTMREEGLGSKPLAEAVQAVLLDTLNMKTDLEVLEQRVFRE
                     RLWKQDLQFVWIRWFMDYPDPHNEYFDTFYGKKTTGKRQAWVNDSFDKELEAGRDTRD
                     PKKRLEHYKKAEEIMQQDVGYVPVAWVVRYAAKKPWVGGIEKNKAGEFVVDGNIYVDM
                     LQHIYITEKG"
     gene            complement(1983..2891)
                     /locus_tag="VNU03_06355"
     CDS             complement(1983..2891)
                     /locus_tag="VNU03_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74114.1"
                     /translation="MALSTPASDTSLAPVIVPREAPRGSSLYRDAWRRLLRNRLAVVG
                     GAVVILLCLVAIFADLIAPQSYTKANFGHLYESPNREFPLGTDQLGRDVLSRLIYGAR
                     VSMLVGLGAQVIIVLIGVPIGALSGYIGGRTDLLLTRFVDVMYAFPRLLFVILVMSML
                     GAGLVNIFIALGLTGWVGIARQTRAQVLSIKEKEFVEGARALGAGFWRVITRHVLPSA
                     LTPIVVSITFGIPEAIFTEAALSFIGVGINPPTPSWGQMVGENQQFLRSYWHLCVFPA
                     IAIAVTMLSFTFFGDGVRDALDPKLK"
     gene            complement(2860..3786)
                     /locus_tag="VNU03_06360"
     CDS             complement(2860..3786)
                     /locus_tag="VNU03_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74115.1"
                     /translation="MLRYVVYRLLWLVPTLLAMALVTFLVMHVTPGSPLDPVAEGANP
                     LSPEAQKNLAEVYGLDKPLYAQFGIFLGKALQGDFGKSFVYKTRTVTEIMVETFPISL
                     LLGSMALALAVVGGVTLGILAAVYQNRSWDYVSVSVATLGVSVPNFVLAVFLIVLFSF
                     VLPIFPTGGWNSPKDWVLPTVTLALAPMGIIARFTRSSMLEVIRSDYIVTARAKGLAE
                     GPVILKHVLKNGFIPVVTLLGPMFAAVGTGSFFVESIFRVPGMGRFFVLSMTGRDYPM
                     IMAVVLVYGAFLALMNLVVDLLYGTLDPRIRY"
     gene            3893..>5494
                     /locus_tag="VNU03_06365"
     CDS             3893..>5494
                     /locus_tag="VNU03_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HWP74116.1"
                     /translation="MAIPLTILPGRHVLDTKPGTTILKSAHASGVDITATCGGRGRCT
                     SCRVKLVAGTAPPPTIMDELQLGDALVREGYRLSCQWPVTEPVTVQIAPPLDDTTFQI
                     LGGERPAGAPRPVSIDAGIRKRAVTVALPKDEHQQTSDVEALVAAAGGTVEDVPASLL
                     KSLPATLRAQDGLVTVTTFGRHVLAIEADDTALHKFGLAIDIGTTSVVTTLMELESGE
                     QLAAVSSLNPQAVFGGDLMSRIAFAQFDAGNLRKLQTRIIGLLNQHIEQITRESGVLA
                     KWIYKVVIVGNTCMHHLLLGIDPSYVGLAPYAPVVRQPLILPARELFLKLSPEARVCL
                     LPLVAGFVGADAVAVALATRLDESPTLRIAVDIGTNGEVLLGSRERLWACSAPAGPAL
                     EGAQIRHGMRAAIGAIDRVEVAAGDLRLHTIGDAPAQGLCGSGLLDAVAALLDLGVLD
                     WTGLIDVDARDRLPAPIRERVLMRGEERLVVLARVGEHGAEREITLTQDDVRQVQLCK
                     GAIASGVAMLQHVAGVPTERVDELML"
BASE COUNT          902 a         1876 c         1881 g          835 t
ORIGIN      
        1 accaacaccg acgattcccc ccatcagatc acggtggcgg gcacgccgct caagaccggg
       61 ttcctgctga agggcgagaa cgcgtccctc accttcaagg acgaaggcgt cttcaactac
      121 aactgcgcgc tgcaccccaa catgaagggc accatccagg tggcgaagta gctccggccc
      181 ctcaccctgc cttctccccc gcggagagag ggcagggtga gagatcgacg tctggctagc
      241 ccttctcggt gatgtagatg tgctgcagca tgtcgacgta gatgttgccg tcgaccacga
      301 actcgcccgc cttgttcttc tcgattccgc ccacccacgg cttcttggcc gcgtagcgca
      361 cgacccaggc caccggcacg tagccgacgt cctgctgcat gatctcctcc gccttcttgt
      421 agtgctcgag ccgcttcttg gggtcgcggg tgtcgcggcc cgcctcgagt tccttgtcga
      481 aggagtcgtt gacccaggcc tgccgcttgc cggtggtctt cttgccgtag aaggtgtcga
      541 agtactcgtt gtgcggatcc gggtagtcca tgaaccagcg gatccacacg aactggagat
      601 cctgcttcca gagccgctcg cggaagacac gctgctcgag gacctcgagg tcggtcttca
      661 tgttgagcgt gtcgagcagc accgcctgca ccgcctccgc cagaggcttg gagcccaggc
      721 cctcctcgcg catcgtcagc gtgatcttgg gccagttctt gccgccctcg tagggcgtgc
      781 ccttgagcgc gtcgagggcc gccttcttgt cgtaccgctg gatggccttg atacccgcgt
      841 cgtcgatggc gcccgggaag ccgggcggga tcatggacca cgccgggatc gcgaagccct
      901 ggctcacctt gaccacgttc tcccggtcga tggcctgggc gaccgccttg cggaccttga
      961 ggttgtcgaa gggcggcttg ctcacctggg gtagcaggta ccaggtgccg gggaacggat
     1021 agcggaacac gtccttgccc atgcgcgggt ccgactgcag ccgcttgaga tcgccggact
     1081 ggagcgcggt catgtcgagc tcgttgttct cgtaggggag cgagccggag gcgaccggga
     1141 cgatcgggat caccactcgg ctgaggtgca cgtccttggc cccgaagtag tgggggttct
     1201 tcttcagcac gatctgcttg ttgtgctccc acgcctcgag ggtgaacggg ccgttgctga
     1261 cgatgttgcc ggcctccgtc cacttgtcgc cgaacttctc caccgcgccc cggtgcgcgg
     1321 gcagcgcggc caggtaggag gcgagcaccg ggaagtagcc gcgcggcccg tcgagggtca
     1381 cctcgagcgt ccagtcgtcc ttggcgcgga ccccgacctg gctcgcgtcg gtgatctgct
     1441 tcttgttgta ggcctccccg ttcttgatgt cgtacaggaa cgaggcgtac ggcgcggcgc
     1501 tcgcggggtc gagctggcgc ttccacgacc actcgaagtc gcgcgccgag accggggagt
     1561 tgtcggacca gcggctgtct ttccgaatgg tgaacgtcca caccgacccg tccttgttgc
     1621 cctcgacctt gctcgccatc cacggcaccg acacgaagtc cgcgttgaag accatcaggc
     1681 ccgcgaagag ggccggcacc ccggagcagt agagatcctt gttgaagtcg tggctggccg
     1741 ggtcgttctg gaaccagccg ccgccgccga agcggaaggc ctgctccttg gccagcttct
     1801 cgttggggcc gggcttgctc ggcttggcgg cctgggcggt ggtggcgaag ccgaggccgc
     1861 cgaacgcggc catcgccccg accaccttca ggaagtcccg gcgaccgacc ccgatggcct
     1921 cgaggcgctt ggcgaagaga tccatctgct catccggaat ccgcatggag gtcctcctgt
     1981 ggttacttca gcttggggtc cagcgcgtcg cggaccccgt cgccgaagaa ggtgaacgag
     2041 agcatggtca ccgcgatggc gatcgcggga aagacgcaga ggtgccagta ggagcggagg
     2101 aactgctggt tctcccccac catctgcccc cacgaggggg tgggcgggtt gatgccgacc
     2161 ccgatgaacg acagggcggc ctcggtgaag atcgcctccg ggatgccgaa ggtgatcgag
     2221 accacgatgg gggtgagcgc gctcggcagg acgtggcggg tgatcactcg ccagaagccg
     2281 gccccgaggg cgcgggcacc ctccacgaac tccttctcct tgatcgacag cacctgggca
     2341 cgggtctggc gcgcgatgcc cacccacccg gtgaggccga gggcgatgaa gatgttgacg
     2401 agcccggccc ccagcatcga catcaccagg atgacgaaga gcaggcgcgg gaaggcgtac
     2461 atcacgtcaa cgaagcgggt gagcaggaga tcggtccgcc ctccgatgta gccggagagg
     2521 gccccgatgg gcaccccgat cagcacgatg atcacctgcg ctccgaggcc caccagcatc
     2581 gagacccgcg cgccgtagat caggcgcgag agcacgtcgc gcccgagctg gtcggtgccg
     2641 agcgggaact cgcggttcgg cgactcgtag aggtggccga agttcgcctt ggtgtaggac
     2701 tggggcgcga tcaggtccgc gaagatcgcg acgaggcaga gcaggatgac cactgcgccg
     2761 cccaccaccg cgagccggtt gcgcagcagg cggcgccacg cgtcgcggta gaggctcgag
     2821 ccccggggcg cctcacgcgg gacgatgacc ggcgcgaggc tagtatcgga tgcgggggtc
     2881 gagagtgcca tagaggaggt ccaccaccag gttcatgagg gcgaggaacg ccccgtagac
     2941 cagcaccacc gccatgatca tcgggtagtc gcgcccggtc atcgagagca cgaagaagcg
     3001 gcccatgccc gggacccgga agatcgactc cacgaagaac gagccggtcc ccaccgcggc
     3061 gaacatgggg ccgagcaggg tgaccaccgg gatgaagccg ttcttgagca cgtgcttgag
     3121 gatgaccgga ccctcggcca ggcccttggc ccgcgcggtc acgatgtagt cggagcggat
     3181 cacctcgagc atgctcgagc gggtgaagcg agcgatgatg cccatcgggg cgagggcgag
     3241 cgtgaccgtc ggcagcaccc agtccttcgg cgagttccac cccccggtcg ggaagatggg
     3301 cagcacgaac gagaagagca cgatcaggaa caccgcgagc acgaagttcg gcacgctcac
     3361 cccgagggtg gccacgctca ccgagacgta gtcccacgag cgattctggt agaccgcggc
     3421 caggatcccg agggtgaccc cacccaccac cgccagcgcg agcgccatgc tgccgagcag
     3481 gagcgagatc ggaaacgtct ccaccatgat ctcggtcacg gtgcgcgtct tgtagacgaa
     3541 cgacttcccg aagtcgccct gcagcgcctt gcccaggaag atcccgaact gggcgtagag
     3601 gggcttgtcg agaccgtaga cctcggcgag gttcttctgg gcctcgggcg agagtgggtt
     3661 ggcgccctcc gcgaccgggt ccagcgggct gcccggagtg acgtgcatca ccaggaaggt
     3721 gacgagcgcc atcgcgagca gcgtcggaac caaccagagg aggcgataga cgacgtagcg
     3781 gagcaaccac accccccgaa gcagtgcccg ccgagccttc tgacgggtta atagggaact
     3841 attggcaaac ccccgcggga agtcaagaaa gaagtggtat cctgcgcccg gaatggcgat
     3901 tccgctgacc atcctgcccg gccgccacgt cctcgacacg aagcccggca ccacgatcct
     3961 gaagtcggcc cacgcctcgg gggtcgacat caccgccacc tgcggcgggc gcggtcgatg
     4021 cacgtcgtgc cgggtcaagc tcgtcgcggg caccgcgccg ccgcccacca tcatggacga
     4081 gctgcagctg ggcgacgcgc tggtgcgcga gggctaccgg ctctcgtgcc agtggccggt
     4141 gaccgagccg gtgaccgtcc agatcgcccc cccgctcgac gacaccacct tccagatcct
     4201 cggcggggag cgcccagcgg gcgcgcctcg gccggtctcg atcgacgcgg gcatccgcaa
     4261 gcgcgcggtg acggtggccc tgcccaagga cgagcaccag cagacctccg acgtggaggc
     4321 cctggtggcc gccgcgggcg gcacggtgga ggacgtgccc gcctcgctgc tcaagagcct
     4381 gcccgcgacg ctccgcgccc aggacggcct cgtcaccgtg accaccttcg gccggcacgt
     4441 cctcgcgatc gaggccgacg acaccgcgct ccacaagttc ggcctcgcca tcgacatcgg
     4501 caccaccagc gtggtcacca ccctgatgga gctcgagtcg ggcgagcagc tcgcggcggt
     4561 ctcgagcctc aacccccagg cggtgttcgg gggcgacctg atgtcgcgca tcgccttcgc
     4621 ccagttcgac gcgggcaacc tccgcaagct gcagacccgc atcatcggcc tgctcaacca
     4681 gcacatcgag cagatcacgc gcgagtccgg cgtgctcgcc aagtggatct acaaggtggt
     4741 gatcgtcggc aacacctgca tgcaccacct gctcctgggc atcgatccgt cctacgtggg
     4801 gctggccccg tacgcgccgg tggtgcggca gcccctgatc ctgcccgccc gcgagctgtt
     4861 cctgaagctg tcgccggagg cgcgggtctg cctgctgccg ctggtggcgg gcttcgtggg
     4921 cgccgacgcg gtcgcggtgg cgctggccac ccgcctcgac gaatcgccca ctctccgcat
     4981 cgcggtcgac atcggcacca acggcgaggt cctgctgggc tcccgcgagc ggctgtgggc
     5041 ctgctcggcc cccgccggcc ccgcgctcga gggggcgcag atccgccacg gcatgcgcgc
     5101 ggccatcggg gccatcgacc gcgtcgaggt cgcggcgggc gacctgcgcc tgcacaccat
     5161 cggcgacgcg cccgcgcagg ggctctgcgg ctcgggcctc ctggacgcgg tggccgcgct
     5221 gctcgacctg ggcgtgctcg actggaccgg cctcatcgac gtggacgccc gcgaccggct
     5281 gcccgcgccc atccgggagc gcgtcctgat gcgcggcgag gagcggctcg tggtgctcgc
     5341 gcgggtgggc gagcacggcg cggagcggga gatcacgctg acccaggacg acgtccggca
     5401 ggtgcagctc tgcaagggcg cgatcgcctc cggggtggcc atgctccagc acgtggccgg
     5461 ggtgccgacc gagcgagtcg acgagctgat gctg
//
LOCUS       DATMCV010000275        10212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_213543, whole genome shotgun sequence.
ACCESSION   DATMCV010000275 DATMCV010000000
VERSION     DATMCV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213543"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VNU03_06370"
     CDS             complement(<1..758)
                     /locus_tag="VNU03_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74117.1"
                     /translation="MAKRLLVTSAGTGPSNNLIRSLRAGHPSMVVIGAHDDPFILQNS
                     TADRRYRVPAAGPGWARALRRIAREEAVDLVIPATDADTVLLSRMRAGLARHLFLPRP
                     SLIDLCADKYRLAGRLHALGLPAPATARVRSLREVGAIFRRLAGGRGGPLWCRVRRGA
                     GSRGAIPVATAAQARSWIAYWRDMRGVPVTAFTLSEYLPGRDLGCQSVWKDGVQLLVK
                     TYERLSYLSSGTQAAEISSVPALTKTVREPEIATL"
     gene            complement(951..1472)
                     /locus_tag="VNU03_06375"
     CDS             complement(951..1472)
                     /locus_tag="VNU03_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HWP74118.1"
                     /translation="MRPLLLLGMAAVLAGCASTPADRGPLSAEAVLKDKEGKQVGVAT
                     MIQTPEGVRIAVTGYRLPPGGHGLHVHAVGLCEPPDFTSAGAHFNPGNKQHGTQNPAG
                     AHAGDLPNLVVAASGEGGIDVTTKAFTLAGGTISLFGDKGTALVVHANPDDEKTDPTG
                     NSGGRIACGVITK"
     gene            complement(1491..4970)
                     /locus_tag="VNU03_06380"
     CDS             complement(1491..4970)
                     /locus_tag="VNU03_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HWP74119.1"
                     /translation="MTTQSGEFSLEAKYTQERGRIFLSGIQALVRLPLDHHRADLRRG
                     LNTATFISGYRGSPLGGFDQTLERQSVLLKAHDVVFSSGLNEDLGATAVYGSQMAGLF
                     PKPKYDGVVGMWYGKAPGVDRTGDLFKHANYAGVGKNGGVLALAGDDPLSKSSTLPSA
                     SEVALYDAFMPTLFPGNVQEILDLGLHGFMLSRTSGLWVGVKIVTNVADEIGTAEVDA
                     ERVSPIIPVVELDGKPFQHQINVNLIPPYGLDMERTIHGARLELARRYAYENKLNRIT
                     VPTPNAWLGILTSGKTYYDVRQALTELGLDEAALRRYGIRILKMGMLFPMEPRVVREF
                     SRGLQEILVVEEKRSFLEMFCKDILYGMTDRPQVVGKFDEEDRPLLQPVGELDSDLVA
                     RAIARRLARRIRIDSVEARIQHLDELKRRPKPFTLARSAFFCSGCPHNRSTVVPEGSV
                     ASAGIGCHGMAMGMDRGIIGVTHMGAEGAQWVGVAPFTETTHLFQNIGDGTFFHSGGL
                     ALNYAVASGVNITYKILYNGAVAMTGGQNAAGALPIPALSRRLEAEGVKKIIITSDDT
                     AKYSGTSIAANAEVWHRDRLLEAQSVLAATRGVTVLIHDQHCAAEKRRLRKRGKMVDP
                     QTRVFINERVCEGCGDCGKKSNCLSVQPLDTEFGRKTAIHQSSCNKDYSCLLGDCPSF
                     LTIVGAGTAPKRERKLTPLEVDLPEPSLKVPTEGFALHMMGIGGTGVVTMNQILGTAA
                     MLDGRHVRGLDQTGLSQKGGPVVSDLKLASATIESANKVSAGSADLYLGFDLLVATDP
                     INLDKTESGRTIAVVSTSQIPTGQMVVDTAVHFPELSSMLMSIDRVTRKDPNVYLDAQ
                     AIAEALFGDHMAVNPIMLGAAYQAGALPVSAASIEKAIRLNGVSVEMNLLAFRWGRMA
                     VVDRAQVESAVAQATTRVAEQPAALSAVAQALVDRVGATGELRRLLEIRVPELIAYQN
                     AAYAGEYVDFVARVAQVEAERAPGQRGLAEAAARYLHKLMAYKDEYEVARLHLDAAVQ
                     AQLQSKFGPRIRTYWHLHPPLLRALGLKKKIRLGSWFAPAFRLLAAMKGLRGTRLDVF
                     GYAAIRQVERALIEEYRQHLETAMVRLSPVTHDLAVKLAELPDEVRGYEHIKMENVER
                     FRDRAQQLIAQIN"
     gene            complement(5060..5698)
                     /locus_tag="VNU03_06385"
     CDS             complement(5060..5698)
                     /locus_tag="VNU03_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWP74120.1"
                     /translation="MRGLLGLLLVVLQLTACAAAPAAPPPGVQSGRVIVAFGDSLTAG
                     FGVPADQTYPARLGERLKREGYDYRVVNAGVSGDTTAGGVRRVDWALQLKPEIVILEL
                     GVNDALRGQKLAGVRANLDQMITRFQAAGARVLVAGMRLPPNYGDRYAEDFHRLYAEV
                     ARAHQAALMPFFLDGVGAVARLNQADGIHPTAEGYAIVVDRLWPYLVPLLQR"
     gene            5720..6751
                     /locus_tag="VNU03_06390"
     CDS             5720..6751
                     /locus_tag="VNU03_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HWP74121.1"
                     /translation="MRTHRLLRLVMVAAGLATWWPLAAPGAAVALEAAQLGVVVNTAD
                     PFSVEIGDYYVRRRGIPRANVVFVTLPTGQDEISRSAFESARAQVQAGLPGRVQALAL
                     AWTTPFRVDCLSVTTAFAFGFDPAFCAQGCHRTRPSPYFDSPSATPFQAWGMRPAMLL
                     AGASVAEAKALIDRGVAADSSFPSGTAYLLSTSDRARNTRAARYSLATALDVPVRVRR
                     IDADALRDRHDVLFYFIGAVTVAGLDTLRFLPGAIADHLTSTGGVLTGQDQMSVLRWL
                     EAGATASYGAVVEPCNFPSKFPQPAVVIARYTRGETAIEAYWKSVAWPGQGVFVGEPL
                     AAPFRPRPR"
     gene            6911..7510
                     /locus_tag="VNU03_06395"
     CDS             6911..7510
                     /locus_tag="VNU03_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74122.1"
                     /translation="MEGPVSSPSEQSELDHIRASLDTRYTSEREDDPETGTPLLGVGI
                     AFAAFVPTFLLIVMGLPYLLAPTSVPVATAPDPATLSLLSTAVMDPSSRSDSRDRSAA
                     SLSELVETRAVSGSWASPDRARATRPGDDEAKPARIPSPDAMSWTRAAAFADDQAAAR
                     LARSLRGQGYRVDLRREDGATLPWVVWIGKPPTPPRPSR"
     gene            7569..8246
                     /locus_tag="VNU03_06400"
     CDS             7569..8246
                     /locus_tag="VNU03_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74123.1"
                     /translation="MDAIMIRVQSLSMRLSSGGRAVDVLTDVSLEVPAGQFVAIAGPS
                     GSGKSTLLGLIAGLDQPTAGRIEVAGVTITGLDEDALARFRGDHVGYVFQSFHLIPTL
                     TAIENVAVPLELAGRADAETRATALLAEVGLAERGHHYPVQLSGGEQQRVAVARALAR
                     RPPLLLADEPTGNLDSATGKQIIELLVGANRRLGSTLVLVTHDAALAAHADRVVTLSD
                     GRIVSDE"
     gene            8243..>10212
                     /locus_tag="VNU03_06405"
     CDS             8243..>10212
                     /locus_tag="VNU03_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74124.1"
                     /translation="MSRFVLRLAWRETRGAWRHFGYFFACITLGVSALVGVGSFADSL
                     ERTVARSAKSLMGGDVEIRSTQPLPLPSLEDPAGAARGVAVTQLRELVAMAQAGPAST
                     QLVELKAVEPGYPFYGALVTDPPGPLDTLIGEGRALVHESFLARLGLRVGDRFRVGQA
                     ELTVAGVIVKEPDRAVGVFSLGPRVMIAAADLDRTGLVRPGSRVRYRTLFRLPEGADA
                     SAFRDRLAERLPAAQRVTTYTQAQPGLRRFWDQLTMYLGLTGLVALMVGGIGVAVSVS
                     AFVKEKLSAIAIMKALGAGWRPLLAAYLLQTALLGLGGSLAGALLGSLVQPLLAPTLT
                     RLLPIELQLSFSLRAVLGGVAMGVGVTLLYALWPLLQIRRVPPALILRHEVEPRLLGR
                     RPWGAAVPIAAGLAALALWQAGSWKIGALFAGGLAGALLLLALGARGVIALARRVRWR
                     APAWRQGAANLHRPGSHAGPVLVSLGLAVMLVVSIALLETSLRSQLKDRSPGQAPAFF
                     FIDIQPDQAESFAALAAGHGADAPAELTPVVRSRLAAVNGAPVAQGEKTRGEEAWYLT
                     REYVLTWAAEPPGHNTVVAGRWWTPAEAAREPLISVEEEIARQLGVGLGDTLTFDVQG
                     VPISARVTSLRHVDWRSLASNFFVIFSPGALEG"
BASE COUNT         1352 a         3714 c         3590 g         1556 t
ORIGIN      
        1 agcgtggcga tctccggctc ccgcaccgtc ttggtgagcg cgggcaccga cgagatctcg
       61 gccgcctggg tcccgctcga caggtaggag aggcgctcgt aggtcttgac cagcagctgg
      121 accccgtcct tccacacgct ctggcacccg aggtcgcggc cgggcaggta ctcgctcagg
      181 gtgaaggcgg tgaccggcac cccgcgcatg tcgcgccagt aggcgatcca gctccgcgcc
      241 tgcgccgcgg tcgccaccgg gatggccccc cgcgagcccg cgccccggcg cacgcggcac
      301 cagagcggcc cgccgcgccc gccggcgagg cgacggaaga tcgcgccgac ctcccggagg
      361 ctccggacgc gcgcggtggc gggagcgggc aggccgagcg cgtggagccg cccggcgaga
      421 cgatacttgt ccgcgcacag gtcgatcagc gacgggcgcg gcaggaagag gtggcgcgcg
      481 aggccggccc gcatccggga gagcaggacg gtgtcggcgt cggtggcggg aatgaccagg
      541 tcgaccgcct cctcgcgcgc gatgcgccgc agggcgcggg cccacccggg cccggcggcg
      601 ggcacccggt atcggcgatc ggcggtggag ttctgcagga tgaacggatc gtcgtgggcg
      661 ccgatgacga ccatcgacgg gtgaccggcg cggagactgc gaatcaggtt gttgctggga
      721 ccggttccgg cgctcgtgac gagaagccgc ttcgccatct cacctccacg tcgtgcgcct
      781 cgaggatgag gcgcggccga tactaggcag cgcgtggaaa gcttgtcaac gcgatacgtc
      841 ggtgtcctta ctgggtgccc gtcctccggc ggcgatccga cctccgaaac gacgagaggg
      901 ccaggtcatt gcgacctggc cctctggcgc cgcgcgcgag tggcggcccg ttacttcgtg
      961 atgactccgc aggcgatgcg accgccgctg ttgccggtgg gatcggtctt ctcgtcgtcc
     1021 ggattggcgt ggaccacgag cgcggtcccc ttgtcgccga acagcgatat ggtgcccccc
     1081 gcgagggtga aggccttcgt ggtcacgtcg atgccgcctt cgccggacgc ggccaccacc
     1141 aggttcggca gatcgcccgc gtgcgctccg gccgggttct gggtcccgtg ctgcttgttg
     1201 cccggattga agtgcgcgcc cgcggacgtg aagtcgggtg gctcgcacag gcccaccgcg
     1261 tggacgtgga gaccgtggcc gcccggtggc agccggtagc cggtcaccgc gatccgcacg
     1321 ccctccgggg tctggatcat cgtggccact cccacctgct tgccttcctt gtccttgagc
     1381 acggcctccg ccgaaagcgg tccccggtcg gccggggtgc tcgcacatcc ggccagcact
     1441 gcggccatac cgagcagcag aagtggtctc accgttgagt gccctcccgt tcagttgatc
     1501 tgcgcaatga gctgctgggc ccggtcgcgg aagcgctcga cgttctccat cttgatgtgc
     1561 tcgtagcccc ggacctcgtc cggcagctcg gcgagcttca cggccaggtc gtgcgtgacc
     1621 gggctgagcc ggaccatcgc ggtctccaga tgctgccggt actcctcgat cagcgcccgc
     1681 tccacctgcc ggatcgcggc gtacccgaag acgtcgagcc gggtgccccg cagccccttc
     1741 atggcggcca ggaggcggaa ggcgggggcg aaccagctcc cgagccggat cttcttcttg
     1801 aggccgagcg cgcgcaggag cggcgggtgc aggtgccagt aggtgcggat cctcggtccg
     1861 aacttcgact gcagctgggc ctgcaccgcc gcgtcgaggt gcagccgcgc cacctcgtac
     1921 tcgtccttgt aggccatcag cttgtggaga tagcgggcgg cggcctccgc caggccccgc
     1981 tggccgggcg cgcgctccgc ctccacctgc gcgacccgcg cgacgaagtc cacgtactcg
     2041 cccgcgtagg ccgcgttctg gtaggcgatc agctcgggca cccggatctc gagcaggcgc
     2101 cgcagctccc cggtggcgcc cacccggtcc accagcgcct gcgcgaccgc actcagcgcg
     2161 gcgggctgct cggcgacccg ggtggtggcc tgggcgaccg cgctctcgac ctgcgcgcgg
     2221 tccaccaccg ccatgcggcc ccaccggaac gcgagcaggt tcatctcgac cgacaccccg
     2281 ttgagacgga tcgccttctc gatcgacgcg gcggagaccg ggagcgcgcc cgcctggtac
     2341 gcggcgccga gcatgatcgg gttgaccgcc atgtgatcgc cgaagagcgc ctccgcgatg
     2401 gcctgggcgt cgaggtagac gttgggatcc ttgcgggtca cccgatcgat gctcatcagc
     2461 atgctgctca gctcggggaa gtgcaccgcg gtgtccacca ccatctggcc ggtgggaatc
     2521 tggctcgtcg agaccaccgc gatggtccgc cccgactccg tcttgtcgag gttgatcggg
     2581 tcggtggcca ccagcaggtc gaagcccagg tagagatcgg cggagccggc cgagaccttg
     2641 ttggcggact cgatcgtggc ggaggcgagc ttgagatcgg agaccaccgg cccgcccttc
     2701 tggctgagcc cggtctggtc gaggccgcgc acgtggcggc cgtcgagcat cgcggcggtg
     2761 ccgaggatct ggttcatggt caccaccccg gtgcccccga tgcccatcat gtgcagcgcg
     2821 aagccctcgg tcggcacctt gagcgacggc tcgggcagat ccacctcgag cggggtcagc
     2881 ttccgttccc ttttgggcgc cgtcccggcg cccacgatgg tgaggaacga cgggcagtcg
     2941 ccgagcaggc acgagtagtc cttgttgcag gacgactggt ggatggcggt cttgcgcccg
     3001 aactcggtgt cgaggggctg gaccgagagg cagttcgact tcttgccgca gtccccgcag
     3061 ccctcgcaca cccgctcgtt gatgaacacc cgggtctgcg gatccaccat cttgccgcgc
     3121 ttgcgcaggc gccgcttctc cgccgcgcag tgctggtcgt ggatgagcac ggtgactccg
     3181 cgggtggccg cgagcaccga ctgggcctcg aggaggcgat cgcgatgcca gacctccgcg
     3241 ttcgccgcga tcgacgtgcc cgaatacttg gcggtgtcgt cgctggtgat gatgatcttc
     3301 ttcacgccct cggcctcgag ccgccggctg agcgctggga tcgggagcgc gcccgccgcg
     3361 ttctgtccgc cggtcatcgc caccgcgccg ttgtagagga tcttgtaggt gatgttgacg
     3421 cccgaggcga ccgcgtagtt gagcgcgagg ccgcccgagt ggaagaacgt cccgtcgccg
     3481 atgttctgga agaggtgcgt cgtctcggtg aagggcgcga cgccgaccca ctgcgcgccc
     3541 tccgcgccca tgtgggtgac cccgatgatg ccccggtcca tgcccatggc catgccgtgg
     3601 cagccgatgc ccgcggaggc cacgctgcct tccggcacca cggtggagcg attgtgcggg
     3661 cagcccgagc agaagaaggc gctgcgggcc agggtgaacg gcttcgggcg gcgcttcagc
     3721 tcgtcgaggt gctggatgcg cgcctccacc gagtcgatgc ggatgcggcg cgcgagccgc
     3781 cgggcgatcg cgcgggccac caggtcggag tccagctcgc cgaccggctg cagcagcggc
     3841 cggtcctcct cgtcgaactt gcccaccacc tgcggccggt cggtcatgcc gtagaggatg
     3901 tccttgcaga acatctcgag gaacgaccgc ttctcctcga cgacgaggat ctcctgcagg
     3961 ccccgcgaga actcccgcac cacccgcggc tccatcggga acagcatgcc catcttgagg
     4021 atccggatgc cgtagcggcg cagcgcggcc tcgtcgaggc cgagctcggt gagggcctgg
     4081 cggacgtcgt agtaggtctt gccgctggtg aggataccga gccacgcgtt cggggtcggc
     4141 accgtgatcc ggttgagctt gttctcgtac gcgtagcgcc gggccagctc gaggcgcgcc
     4201 ccgtggatgg tgcgctccat gtcgagaccg tagggcggga tcaggttcac gttgatctgg
     4261 tgctggaagg gcttgccgtc gagctccacc accgggatga tcgggctcac ccgctccgcg
     4321 tcgacctccg cggtgccgat ctcgtccgcc acgttggtga cgatcttgac cccgacccac
     4381 aaccccgacg tacgggagag catgaacccg tgcaggccca ggtcgaggat ctcctgcacg
     4441 ttgccgggga acagcgtcgg catgaacgcg tcgtagaggg cgacctcgga ggcgctcggc
     4501 agcgtagaag atttcgaaag tggatcatcc cccgccagcg cgagcactcc gccgttcttg
     4561 cccacccccg cgtagttggc gtgcttgaac aggtcgccgg tgcggtcgac gccgggggcc
     4621 ttgccgtacc acatgccgac cacgccgtcg tacttgggct tcgggaaaag gcccgccatc
     4681 tggctgccgt acaccgcggt cgcgcccagg tcctcgttga ggcccgagga gaagaccacg
     4741 tcgtgggcct tgagcagcac cgactggcgc tcgagggtct ggtcgaagcc tccgagcggg
     4801 gagccccggt agccggagat gaacgtcgcg gtgttgaggc cacgccggag atccgctcga
     4861 tggtgatcga gcggcagccg caccagcgcc tggatgccgg acaggaagat gcgcccgcgc
     4921 tcctgggtgt acttggcctc gagcgagaac tccccggact gtgtggtcat gagctcccct
     4981 tggggcgacc gcagcgttta gaacgtcctg acgacggcca tatcgtagtc gggaaaggcg
     5041 tttctggcaa cacgatcccc tagcgctgca ggaggggcac gagatacggc cagagccggt
     5101 cgaccacgat cgcgtaccct tccgcggtcg gatggatgcc gtcggcctgg ttgagccggg
     5161 ccaccgcgcc gaccccgtcg aggaagaacg gcatgagcgc cgcctggtgg gcccgggcca
     5221 cctccgcgta gaggcgatgg aagtcctccg cgtagcggtc gccgtagttc ggcggcagcc
     5281 gcatgcccgc caccagcacc cgcgcccccg cggcctggaa gcgcgtgatc atctgatcca
     5341 ggttggcgcg aaccccggcg agcttctggc cgcggagcgc gtcgttgacc ccgagctcca
     5401 ggatcacgat ctcgggcttg agctggagcg cccagtcgac gcgccgcact ccgcccgcgg
     5461 tggtgtcgcc cgatactccc gcgttcacca cccggtagtc gtagccctcc cgcttgagac
     5521 gctccccgag ccgggccggg tacgtctgat ccgccggaac cccgaacccg gcggtgaggc
     5581 tgtcgccgaa ggccacgatg acgcgtcccg actggacgcc gggcggtggg gcagcgggcg
     5641 ccgccgcaca ggcggtgagc tggaggacga cgaggagcag gccgagaagg ccgcgcatga
     5701 ggccagtgta gtatgagccg tgcgcacgca ccggctgctc cggctggtga tggtggcggc
     5761 cggcctcgcc acgtggtggc ccctcgcggc gcccggcgcg gccgtcgcgc tcgaggccgc
     5821 gcagctcggc gtggtcgtca acaccgccga tcccttcagc gtggagatcg gcgactacta
     5881 cgtgcggcgc cgcgggatcc cgcgcgccaa cgtggtgttc gtcaccctcc cgaccgggca
     5941 ggacgagatc agccgctcgg ccttcgagtc ggcccgtgcc caggtgcagg cgggtcttcc
     6001 cgggcgcgtg caggcgctcg cgctcgcgtg gaccaccccg ttccgggtgg actgcctgtc
     6061 ggtgaccacc gcgttcgcgt tcggcttcga tcccgcgttc tgcgcgcagg gctgccaccg
     6121 cacgcggccc agcccctact tcgactcgcc gagcgcgacc ccgttccagg cgtggggcat
     6181 gcgcccggcc atgctactgg ccggggccag cgtggcggag gcgaaggccc tgatcgatcg
     6241 cggcgtggcc gccgactcct cctttccgtc cggcaccgcg tatctcctgt caacctccga
     6301 ccgagcccgc aacacccgcg cggcccgcta ctcgctcgcc accgcgctcg acgtcccggt
     6361 tcgcgtgcgc cgcatcgacg ccgacgcgct gcgggaccgg cacgacgtgc tcttctactt
     6421 catcggcgcg gtgacggtag cggggctcga caccctccgc ttcctgcccg gggccatcgc
     6481 cgaccacctc acctccaccg gcggcgtgct gaccggacag gaccagatga gcgtgctgcg
     6541 ctggctcgag gcgggcgcca ccgccagcta cggcgcggtc gtggagccgt gcaacttccc
     6601 gtcgaagttc ccgcagcccg cggtggtcat cgcccgctac acgcgcgggg agaccgcgat
     6661 cgaggcctac tggaagagcg tggcctggcc cggccagggc gtgttcgtgg gcgagcccct
     6721 cgccgccccg ttccgtccgc gccctcgcta gccggcccgg taacgactta tccacagatt
     6781 gtccaggtta tccacaggcc gagatgccaa tgtgggcggt gagttacagc tcacaggcac
     6841 cggatcgtga ttgacaatca gccaatgatc acttatgttc tggccgcccc aacttcgcag
     6901 gaggccgcac gtggaaggtc ccgtctccag cccgtcagag cagagcgagc ttgaccacat
     6961 ccgtgcctcg ctcgacaccc gctacaccag cgagcgcgag gacgatccgg agaccgggac
     7021 tcccttgctg ggcgtcggga tcgcgttcgc cgcattcgtt cccacgttcc tgctgatcgt
     7081 catggggctg ccatatctgt tagcgcccac ctcggttccg gtcgccaccg ccccggaccc
     7141 ggcgacgctc tcgctgctct cgaccgcggt gatggacccc tcctcccggt ctgactcgcg
     7201 cgatcgatcg gccgcgtcgc tgtccgagct cgtggagacc cgagcggtca gcggcagctg
     7261 ggcgagcccc gaccgcgcgc gcgcgaccag acccggcgac gacgaggcca agccggcccg
     7321 catcccctcc ccggacgcga tgtcgtggac gcgcgcggcc gcgttcgcgg acgatcaggc
     7381 ggccgcgcgg ctcgccagga gcctgcgcgg ccagggttat cgcgtggacc tccgccggga
     7441 ggacggcgcg acgttgccat gggtcgtctg gatcggcaag ccgcccactc cgccccgccc
     7501 ctccaggtga tcctctcgct cttgtcctag cgaccgcccg ccccgcctcg gcggtcgcgg
     7561 cgtgtagtat ggacgccatc atgatccgcg tgcagtcgct gtccatgcgt ctctcgagcg
     7621 gcggccgtgc ggtcgacgtg ctcaccgacg tgtcgctcga ggtgccggcc gggcagttcg
     7681 tcgccatcgc ggggccgtcg ggcagcggca agtcgacgct gctggggctg atcgccgggc
     7741 tcgaccagcc gaccgcgggc cgcatcgagg tggcgggcgt cacgatcacc ggcctcgacg
     7801 aggacgcgct cgcgcgcttc cggggcgacc acgtgggcta cgtcttccag tccttccacc
     7861 tgatcccgac cctgaccgcg atcgagaacg tggcggtgcc gctcgagctg gccggccggg
     7921 ccgacgcgga gacgcgggcg acggcgctgc tcgccgaggt gggcctcgcc gagcgcggcc
     7981 atcactaccc ggtgcagctc tcgggcggcg agcagcagcg ggtcgcggtg gcgcgcgcgc
     8041 tcgcccggcg gccgcccctg ctcctggccg acgagcccac cggcaatctc gactccgcga
     8101 ccggcaagca gatcatcgag ctgctggtgg gcgccaaccg acgcctcggc agcaccctgg
     8161 tgctggtcac ccacgacgcc gcgctcgccg cccacgccga ccgggtcgtc accctgagcg
     8221 atggccgcat cgtgagcgac gagtgagccg cttcgtcctc cggctcgcct ggcgcgagac
     8281 ccgcggcgcg tggcggcact tcggctactt cttcgcgtgc atcaccctcg gggtgagcgc
     8341 gctcgtcggg gtcggcagct tcgccgacag cctcgagcgc accgtggccc gctccgccaa
     8401 gtcgctgatg ggcggcgacg tcgagatccg cagcacccag ccccttcccc tgccgtcgct
     8461 cgaggacccg gccggggccg ctcgcggcgt cgcggtcacg cagttgcgcg agctcgtggc
     8521 gatggcccag gcgggccccg cgtcgaccca gctcgtcgag ctcaaggcag tggagccggg
     8581 ctacccgttc tacggcgcgc tggtgaccga cccgccggga ccgctcgaca cgctcatcgg
     8641 cgagggccgg gcgctcgtgc acgaatcctt cctggcccgg ctgggcctgc gggtgggcga
     8701 tcgattccgg gtgggccagg ccgagctgac cgtcgcgggc gtcatcgtga aggagcccga
     8761 ccgcgcggtc ggggtcttct cgctcggccc gcgcgtgatg atcgccgccg ccgatctgga
     8821 ccgcaccggg ctcgtccgcc cgggcagccg cgtgcgctac cgcaccctct tccgcctgcc
     8881 ggagggcgcg gacgcgagcg cattccgcga ccggctcgcg gagcgcctgc ccgcggccca
     8941 gcgggtaacg acgtacaccc aggcccagcc cgggctccgg cgcttctggg accagctcac
     9001 gatgtacctc gggctcaccg ggctggtcgc cctcatggtc ggcggcatcg gcgtcgcggt
     9061 cagcgtgagc gccttcgtca aggagaagct ctccgcgatc gcgatcatga aggcgctcgg
     9121 cgccggctgg cggccgctgc tcgccgccta cctgctccag accgcgctgc tgggcctggg
     9181 cggcagcctg gccggcgcgc tgctcggcag tctcgtccag ccgctgctcg cccccaccct
     9241 gacccggctc ctgccgatcg agctccagct ctccttctcc ctccgcgcgg tcctgggcgg
     9301 cgtggcgatg ggcgtgggcg tgaccctgct ctacgcgctc tggccgctgc tccagatccg
     9361 gcgcgtgccg cccgccctga tcctgcgcca cgaggtcgag ccgcgcctgc tcggccgccg
     9421 gccgtgggga gcggcggtgc cgatcgcggc cggcctggcc gcactcgcgc tctggcaggc
     9481 cggctcctgg aagatcggcg cactgttcgc gggcggcctc gccggcgcgc tcctgctcct
     9541 ggccctgggc gcgcgcggcg tcatcgcgct ggcccgccgg gtgcgctggc gcgcgcccgc
     9601 ctggcggcag ggagcggcca acctccaccg gccgggcagc cacgcagggc cggtgctcgt
     9661 gtcgctcggc ctcgcggtga tgctggtcgt ctcgatcgcg ctgctcgaga ccagcctccg
     9721 ttcccagttg aaagatcgga gcccgggcca ggcacccgcg ttcttcttca tcgacatcca
     9781 gcccgaccag gccgagtcgt tcgcggccct cgcggccggg cacggcgccg acgcgcccgc
     9841 cgagctgacc ccggtggtgc gctcccggct cgccgcggtc aacggcgcgc cggtggccca
     9901 gggcgagaag acgcgtggcg aggaggcgtg gtacctcacg cgcgagtacg tgctcacgtg
     9961 ggccgccgag cctcccggcc acaacaccgt cgtggccggg cgctggtgga cgcccgcgga
    10021 agccgcgcgc gagcccctga tctcggtgga ggaggagatc gcgcgccagc tcggagtcgg
    10081 cctcggcgac accctcacct tcgacgtcca gggcgtgccg atctcggcgc gggtgacgag
    10141 cctccgccac gtggactggc gcagcctcgc ctccaacttc ttcgtgatct tctcgcccgg
    10201 cgccctcgag gg
//
LOCUS       DATMCV010000276         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_880177, whole genome shotgun sequence.
ACCESSION   DATMCV010000276 DATMCV010000000
VERSION     DATMCV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880177"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VNU03_06410"
     CDS             complement(<1..507)
                     /locus_tag="VNU03_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HWP74125.1"
                     /translation="MKTRKIRKELARLAARYDVRKGHKFRLEDVDPGDTSGIESEAQA
                     LKLLSRSTEVLSHMQETLYAQDRWALLLIFQGMDAAGKDGAIKHVMSGVNPQGCDVRS
                     FKAPSPEELDHDYLWRAHKACPERGRIGIFNRSYYEEVLVVRVHGELLAAERLPEPLM
                     SKHIWDERY"
     gene            666..2084
                     /locus_tag="VNU03_06415"
     CDS             666..2084
                     /locus_tag="VNU03_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HWP74126.1"
                     /translation="MSKNTPVASYSLTVRLRIANQPGMLGKVTSAIGEAGGDIGGVDL
                     VEAGSKEMTRDIGFKASDEAHGQAIVDRLRTVPGVTVVNVSDRTFLMHLGGKIEVNGR
                     VTVKTRDDLSMAYTPGVARVCMAIHDDPEKVYNLTIKQHTVAVVTDGSAVLGLGDIGP
                     EAALPVMEGKCLIFKSFAGVDAFPIALATKDVDEIVRTVKAISPVFGGINLEDISAPR
                     CFEIEERLQRDLDIPVFHDDQHGTAVVTLAALLNALKVVNKKLSEVKIVFTGAGASAI
                     ATAKLLMLEGARNIIACDRAGALYRGRTENMNPMKTWFAEHTNAEGIRGAVREAMVGA
                     DVFIGLSGPGVVKVDDLGAMHRDAIVFAMANPTPEIMPEEAGKYVKVMATGRSDYPNQ
                     INNSCGFPGFFKGLFDVRARRVNDEMKLAAAHALANIVSDSELGEEYITPSMFDARVV
                     PAVASAVADAAVRTGVARRKRG"
     gene            2145..>2546
                     /locus_tag="VNU03_06420"
     CDS             2145..>2546
                     /locus_tag="VNU03_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74127.1"
                     /translation="MKKVLAAVLAVLVVLGAGLYFFVARPLLRPSEVTAVAERALATD
                     DLLLLAGINVKQAVFLEKWLLGSPRVTPVAATPPPAVADRSLLDHLRAAGVDPRHDVD
                     HALYALYPADAAGSRHAAILIGRFNPAAVNTY"
BASE COUNT          391 a          903 c          866 g          386 t
ORIGIN      
        1 gtagcgctcg tcccagatgt gcttgctcat gagcggctcc ggcaaccgct cggccgcgag
       61 cagctccccg tgaacccgga ccaccagcac ctcctcgtag taggagcggt tgaagatccc
      121 gatccgcccg cgctccgggc acgccttgtg ggcccgccag aggtagtcgt ggtcgagctc
      181 ctcggggctc ggcgccttga aggatcgcac gtcgcagccc tgcggattga ccccggacat
      241 gacgtgcttg atcgcgccgt ccttgcccgc cgcgtccatg ccctggaaga tcagcaggag
      301 ggcccaccgg tcctgggcgt agagcgtctc ctgcatgtgg ctcagcacct cggtgctccg
      361 cgagaggagc ttgagcgcct gggcctccga ctcgatgccg ctcgtgtcgc ccggatcgac
      421 gtcctcgagg cggaacttgt ggcccttccg cacgtcgtat cgcgcggcca ggcgggccag
      481 ctccttccgg atcttgcgcg tcttcatcgt ggaggcagtc tagtacggaa ggcgagcgct
      541 tgccttccgc ggcgtccgtc gactattctt ggccaccccg tgaccccggc cggccgtgac
      601 gcgctgcgcg gccgttcccc atgtttccag gtgcgcacca catcggcaag gaggctcgcc
      661 acgccatgag caagaacacc ccggtcgcaa gctatagcct caccgttcgc cttcgcatcg
      721 ccaaccagcc cggcatgctc ggcaaggtga cctcggccat cggcgaggcg gggggcgaca
      781 tcgggggggt ggacctggtg gaggcgggct cgaaggagat gacgcgcgac atcgggttca
      841 aggcctccga cgaggcccac ggacaggcca tcgtcgatcg gctgcgcacg gtccccggcg
      901 tgacggtggt gaacgtgtcc gaccggacct tcctcatgca cctgggcggc aagatcgagg
      961 tcaacggccg ggtgacggtg aagacccgcg acgacctgtc gatggcctac acccccgggg
     1021 tggcgcgggt gtgcatggcg atccacgacg atccggagaa ggtctacaac ctgacgatca
     1081 agcagcacac ggtggccgtc gtcaccgacg gctccgcggt gctgggcctg ggcgacatcg
     1141 ggcccgaggc cgcgctcccg gtcatggaag gcaagtgcct catcttcaag tccttcgcgg
     1201 gagtcgacgc gttcccgatc gcgctcgcca ccaaggacgt cgacgagatc gtgcgcaccg
     1261 tgaaggcgat ctcgcccgtc ttcggcggca tcaacctgga ggacatctcg gcgccgcggt
     1321 gcttcgagat cgaggagcgc ctgcagcgcg acctcgacat cccggtcttc cacgacgacc
     1381 agcacggcac cgcggtggtg accctcgccg cgctcctcaa cgcgctgaag gtcgtcaaca
     1441 agaagctcag cgaggtgaag atcgtcttca ccggcgcggg ggcgagcgcg atcgccaccg
     1501 ccaagctcct gatgctggag ggcgcccgca acatcatcgc ctgcgaccgg gccggcgcgc
     1561 tctaccgcgg ccggaccgag aacatgaacc cgatgaagac gtggttcgcg gagcacacca
     1621 acgcggaggg catccgcggg gcggtgcggg aggcgatggt cggggccgac gtgttcatcg
     1681 gcctgagcgg acccggggtg gtgaaggtgg acgatctcgg cgcgatgcac cgcgacgcca
     1741 tcgtcttcgc gatggcgaac cccacccccg agatcatgcc ggaggaggcc gggaagtacg
     1801 tgaaggtcat ggccaccggc cggtcggact acccgaacca gatcaacaac tcgtgcggct
     1861 tcccgggctt cttcaagggc ctgttcgacg tgcgggcgcg ccgggtcaac gacgagatga
     1921 agctggccgc cgcgcacgcg ctcgccaaca tcgtgagcga cagcgagctc ggcgaggagt
     1981 acatcacccc ctcgatgttc gacgcgcgcg tggtgcccgc ggtggcctcc gcggtggccg
     2041 acgcggcggt gcggaccggg gtggcccgga ggaagcgggg ctagacctcc tccccggcaa
     2101 caaatcgctg cggtcccgcg aagccgctcc cgtaccatcg gcgcatgaag aaggtgctgg
     2161 ccgcggtgct cgcggtcctg gtggtgctcg gggccggcct gtacttcttc gtggcccgcc
     2221 cgctgctccg gccctccgag gtgaccgcgg tcgcggagcg ggcgctcgcc accgacgacc
     2281 tcctgctgct ggccgggatc aacgtcaagc aggcggtctt cctcgagaag tggctcctcg
     2341 gctcgccgcg cgtcaccccg gtcgcggcca cgccgccgcc cgcggtggcg gaccgctccc
     2401 tgctcgacca cctccgcgcg gcgggcgtgg atccccggca cgacgtcgat cacgcgctct
     2461 acgccctcta tccggccgac gccgccggct cccggcacgc cgcgatcctg atcggccgct
     2521 tcaaccccgc cgcggtcaac acctat
//
LOCUS       DATMCV010000277         5154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_481273, whole genome shotgun sequence.
ACCESSION   DATMCV010000277 DATMCV010000000
VERSION     DATMCV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481273"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VNU03_06425"
     CDS             complement(<1..554)
                     /locus_tag="VNU03_06425"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HWP74128.1"
                     /translation="MDIAIPLFEGVTALDAIGPYEVLSRLPGARVRFIAVTAGPYRTD
                     NRQLTLMADEPLSAVPHPEVVMVPGGFGTRALEAPNPLLDWIRTAHATSQWTTSVCTG
                     SMLLAAAGILKGLEATTHWMSLDRLPAYGARPTLKRVVEQGKVITAAGVSSGIDMALT
                     LAAHIAGDDVAQAIQLGIEYDPQPP"
     gene            complement(567..1235)
                     /locus_tag="VNU03_06430"
     CDS             complement(567..1235)
                     /locus_tag="VNU03_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWP74129.1"
                     /translation="MASTLLRPGDAAPSVTLPAVNREGQVSLDDYRGRSPVLIALFRG
                     LHCPFCRRQLVQLSTTQDKLKAFGVETVAVVNTPLERARLYFKYRPARVLLTADPEAA
                     THRSFGVPAGEVVEKESEASWAQGKLTMGQLLAMKIDPTGELPEPQNPFAAMETLNKK
                     DGFEVTEIDGQIAHAHGTQMTGHFLIDREGIIRWEHIECAERLEDLSKFPTDEEILQA
                     ARGL"
     gene            complement(1272..4283)
                     /locus_tag="VNU03_06435"
     CDS             complement(1272..4283)
                     /locus_tag="VNU03_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP74130.1"
                     /translation="MLFADLAGFTSLSEGLDPEDVRGIQADLFREMSASIEQYEGFVE
                     KFVGDAVMAVFGAPTAHEDDPERGLRAALMMRDRMGTLNRRWERRVGRPLALHIGVNT
                     GPVVAGRIGSTADAAYAVTGDTVNTAARLQSAAPSGEILIGDATYQLTHHVFSFAPRE
                     EVALKGKSEPVGVHRLLGPLAAPRSARGLEALGLAAPLVGRERELARMEAAFADMRAG
                     RAQVLSLIGEPGAGKSRLQREFFARLESDGRLEGVTIRRAACSALGEQTYGTAAELLR
                     DAYGVEHGDSVEVVRGKLAAGLETLGVDGENRAAMAAALARVFGLEQDDARVRHLEPE
                     QLKRQIFMVVRVLVERRLAASPLVLLVEDLHWADAASIELLGAVADRLADRPLLVLLM
                     YRPTLEPDALGTSHTPHTAIEVTPLSRSGSEDLLAAWFGDATHLFPEHLRELILERTG
                     GNPLYLEEVVRALVAAGVLVRDGLVWRCTAEAVTAQVPATLHGLLLARLDRLAAAERR
                     VIQEAAVIGPRFEVPLLKVVSAEPGAVDVALDALASADLVTPGPDHRFRHGLLQEIVY
                     QNILVTRRTELHTRVGTALEAQGAGDRESLERLVALGHHWSLGADKRRGARYLTGAAD
                     WARGMYANADAIQHYQRALDALEQCDEPAAAAQIETERLVARERMADLLGPTGRRAAA
                     LGEYEIVEAGYRAGGDAAAQARILRKMGGLHWDAGARARALGCFEAGLALLGDHREHI
                     EQAHLYQEMGRQAFRSGDSPRAVEWAEQARLHAERLAADPALDADGRVEAMTAAAQSH
                     NTLGVAYARLGRLEEAVGHIERSVALARETGMLQAACRGLANLSVLYSTLDPARAIET
                     CAAGLEMAKKIGDLGFQSRLYTNLAVAYCALTNRCDEQGIGAAHAAIDLDRRLGQLDH
                     LAVPLIVLGQIYQCHGEPTRAIAHYREALVLAEEADEPQLLFPCYDGLATVHLDLGDE
                     VQAEEFMVKAQAVCERAGLEPDALVVLPFLD"
     gene            complement(4424..4915)
                     /locus_tag="VNU03_06440"
     CDS             complement(4424..4915)
                     /locus_tag="VNU03_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP74131.1"
                     /translation="MSVSGVGLKRLQHLVLWVSNVDRSVRFYCDVLGFEVKTRYPNAA
                     FLRIAGSSEDHHLGLFAQPGVHGVDEGAVRMYHSAWEVGELTDLVRARKRLIEAGSLV
                     GSSDHGVSLSLYAKDPDGLEFEVFWTVPGGRSVGTRALDLESELSRRGIPMPQEIDVA
                     PST"
     gene            complement(5000..5137)
                     /locus_tag="VNU03_06445"
     CDS             complement(5000..5137)
                     /locus_tag="VNU03_06445"
                     /inference="COORDINATES: protein motif:HMM:NF021125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74132.1"
                     /translation="MERVKKELPHGEVTVRPVAGGLKVPGADTIIACAAITVSVEYPG
                     R"
BASE COUNT          712 a         1879 c         1825 g          738 t
ORIGIN      
        1 ggcggctgcg gatcgtactc gatacccagc tggatggcct gcgccacgtc gtcgcccgcg
       61 atgtgggcgg ccagcgtgag cgccatgtcg atgccggagg acacgcccgc tgcggtgatc
      121 accttgccct gctccaccac gcgcttgagg gtcggccgcg cgccgtacgc gggcaggcgg
      181 tcgagcgaca tccagtgggt ggtggcctcg agtcccttca gaatgccggc ggcggccagc
      241 agcatggagc cggtgcagac cgacgtggtc cactggctcg tcgcgtgggc cgtgcggatc
      301 cagtccagca gtgggttcgg cgcctcgagc gcgcgcgtcc cgaagccgcc cggcaccatg
      361 accacctccg gatgcggcac cgccgacagc ggctcgtccg ccatcagggt gagctggcgg
      421 ttgtcggtgc ggtaggggcc cgcggtcacc gcgatgaagc ggacccgggc gccgggcagg
      481 cgcgagagca cctcgtaggg gccgatagcg tcgagcgctg tgacgccctc gaagagcggg
      541 atcgcgatgt ccatggggat gatctcctag aggccgcggg ccgcttgcag tatttcctcg
      601 tcggtcggga acttggagag atcctcgagg cgctccgcgc actcgatgtg ctcccaccgg
      661 atgataccct cccggtcaat gaggaagtgc ccggtcatct gggtgccgtg cgcgtgcgcg
      721 atctgtccgt cgatctcggt cacctcgaac ccgtccttct tgttcagggt ctccatcgcc
      781 gcgaaggggt tctggggctc gggcagctcc ccggtgggat ctatcttcat cgcgagcagc
      841 tggcccatcg tgagcttgcc ctgggcccac gaggcttccg actccttctc gacgacctcc
      901 ccggccggca ctccgaacga ccggtgcgtg gcggcctcgg ggtccgcggt gagcaacaca
      961 cgcgcggggc ggtacttgaa gtagagccgc gcgcgctcca gcggagtgtt gaccaccgcc
     1021 accgtctcca cgccgaacgc cttgagcttg tcctgcgtgg tgctcagctg tacgagctgg
     1081 cgacgacaga aggggcagtg cagcccgcgg aacagggcga tcaggaccgg gctgcgcccg
     1141 cgatagtcgt cgagcgagac ctggccttct cggttcaccg cgggaagcgt gacggaagga
     1201 gcggcatcac cgggacgaag gagtgtagac gccatcagag ggctgccttt ctggttcggg
     1261 ttgtgttcgc gtcaatccag gaacggcagc accaccagcg cgtcgggttc gagcccggcc
     1321 cgctcgcaca ccgcctgggc cttcaccatg aactcctcgg cttgaacctc gtcgcccagg
     1381 tccaggtgca cggtggcgag gccatcgtag cagggaaaca gcagctgcgg ctcgtcggcc
     1441 tcctccgcga ggacgagcgc ttcgcggtag tgcgcgatcg cgcgggtcgg ctcgccgtgg
     1501 cactggtaga tctgcccgag cacgatgagc ggcaccgcga ggtgatcgag ctggccgagg
     1561 cgccggtcga gatcgatggc ggcatgcgcc gcgccgatgc cctgctcgtc gcagcggttg
     1621 gtgagcgcgc agtaggccac cgcgagattc gtgtagaggc gcgactggaa cccgaggtcg
     1681 ccgatcttct tggccatctc gaggccggcc gcgcaggtct cgatggcccg ggccggatcg
     1741 agcgtgctgt agagcacgct caggttcgcg agcccgcgac aggcggcctg gagcatgccg
     1801 gtctcccggg cgagggccac actgcgctcg atgtgcccga ccgcctcctc cagccgcccc
     1861 aggcgggcat acgcgacccc gagcgtgttg tggctctgcg cggcggcggt catcgcctcc
     1921 acgcgtccgt ccgcgtcgag cgcggggtcg gccgcgagcc gctcggcgtg cagccgcgcc
     1981 tgctccgccc actcgacggc ccgcgggctg tcgccgctgc ggaaggcctg ccggcccatc
     2041 tcctggtaga ggtgcgcctg ctcgatgtgc tctcgatggt cgccgagcag ggcgagcccg
     2101 gcctcgaagc acccgagcgc ccgggcccgc gcgcccgcgt cccagtggag gccgcccatc
     2161 ttgcgaagga tccgcgcctg ggccgccgcg tctccccccg cgcggtatcc ggcctcgacg
     2221 atctcgtact cgccgagcgc ggctgcccgc cgaccggtcg ggccgagcag atcggccatc
     2281 cgctcccggg ccacgagccg ctccgtctcg atctgggcgg ccgccgccgg ctcgtcgcac
     2341 tgctcgagcg cgtcgagggc ccgctggtag tgctggatcg cgtccgcgtt ggcgtacatg
     2401 ccgcgcgccc agtcggcggc ccccgtcagg taccgcgcgc ctcgccgctt gtcggcgccg
     2461 agggaccagt ggtggccgag ggccaccagc cgttcgaggc tctcgcggtc gcccgcgccc
     2521 tgggcttcga gggcggtccc cacgcgggtg tgcagctcgg tgcgccgggt cacgaggatg
     2581 ttctggtaca cgatctcctg gagaaggccg tggcggaacc ggtgatccgg ccccggcgtc
     2641 accaggtcgg ccgaggcgag cgcgtcgagc gccacgtcca ccgcgcccgg ctcagccgag
     2701 accaccttca gcagcgggac ctcgaagcgg ggcccgatca ccgccgcctc ctggatgacc
     2761 cgtcgctccg cggcggcgag ccggtccagc cgcgccagca gcagcccgtg cagcgtcgcg
     2821 ggcacctgcg cggtcacggc ctccgcggtg cagcgccaca ccaggccgtc gcggaccagc
     2881 acccccgccg cgaccagcgc gcgcaccacc tcctcgaggt acagcgggtt gcccccggtc
     2941 cgctccagga tcagctctcg caggtgctcc gggaagagat gcgtcgcgtc tccgaaccac
     3001 gcggccagga ggtcctcgct gccggaccgc gacagcggcg tgacctcgat cgcggtgtgc
     3061 ggggtgtggc tcgtgccgag cgcgtccggc tcgagggtcg gtcgatacat caggaggacc
     3121 aggagcggac gatcggcgag tcggtccgcg accgcgccga gcagctcgat ggaggcggcg
     3181 tcggcccagt gcagatcctc gacgagcagc accaggggac tggcagcgag ccggcgctcg
     3241 accaagaccc gcaccaccat gaagatctgg cgcttgagct gctccggctc caggtgacgg
     3301 acgcgcgcgt cgtcctgctc gagcccgaag acccgggcga gcgcggccgc catcgccgcg
     3361 cggttctccc cgtcgacccc gagcgtctcc aggcccgccg cgagcttgcc gcggacgacc
     3421 tcgacggagt cgccgtgctc caccccgtag gcgtcgcgca gcagctcggc ggccgtccca
     3481 taggtctgct cccccagcgc cgagcacgcg gcgcgccgga tcgtgacgcc ttccaggcgg
     3541 ccgtccgact cgagacgcgc gaagaactcc cgctgcagtc gcgacttccc ggcccccggc
     3601 tcgccgatga ggctcagcac ctgggcgcgc cctgcccgca tgtccgcgaa ggccgcctcc
     3661 atccgggcga gctcgcgctc gcggcccacg aggggcgcgg ccagcccgag ggcctcgagg
     3721 ccgcgggccg agcggggcgc cgcgagcggg cccagcaggc gatgcacccc caccggctcg
     3781 ctcttgccct tcagcgccac ctcctcgcgc ggggcgaagg agaagacgtg gtgggtgagc
     3841 tgatacgtcg cgtcgccgat caggatctcg cccgagggcg ccgcgctctg caggcgcgcg
     3901 gcggtgttga ccgtgtcgcc ggtcaccgcg taggccgcgt cggcggtcga gccgatccgc
     3961 cccgccacca ccggaccggt gttgaccccg atgtgcagcg cgagcggcct gcccacccgc
     4021 cgctcccagc ggcggttgag cgtccccatc cgatcgcgca tcatgagggc ggcgcgcagg
     4081 ccgcgctcgg gatcgtcctc gtgcgcggtg ggagcgccga agacggccat caccgcgtcg
     4141 ccgacgaact tctcgacgaa cccctcgtac tgctcgatcg aggcggacat ctcgcggaac
     4201 aggtcggcct ggatcccccg cacgtcctcg gggtcgagtc cttcgctcag cgaggtgaag
     4261 ccggccagat ccgcgaagag cacggtgacc ggccgccggt cgccctcggg cgtggagggc
     4321 gctgagggcg gaggcgaagg cgccggcgcg ggggccagct tggctccgca cttcggacag
     4381 aatgcgaagt cggggggtgc ttcgaagcta caaccgctac acgtcatgtg gacggagcga
     4441 cgtcgatctc ctggggcatg gggatgccgc gtcgggacag ctcgctctcc agatcgaggg
     4501 cgcgggtgcc caccgagcgg ccccccggca ccgtccagaa cacctcgaac tcgaggccgt
     4561 cgggatcctt ggcgtagagc gacaggctca ccccgtgatc cgacgagccc acgagcgagc
     4621 ccgcctcgat gaggcgcttc cgcgcccgca ccaggtcggt cagctcgccc acctcccacg
     4681 ccgagtggta catgcgcacc gcgccctcgt caacgccgtg gacgccgggc tgggcgaaga
     4741 ggcccaggtg gtggtcctcg ctactccccg cgatgcgcag gaacgcggcg ttgggatagc
     4801 gggtcttcac ctcgaagccg aggacgtcac agtagaagcg cacgctgcga tcgacgttcg
     4861 acacccagag caccaggtgc tgcagccgct tgagcccgac gccgctgacg ctcattgcgc
     4921 gacctccaac ggtaatgcgc ccggttcccg ggcgctcatt gcgcgacctc cgacggtgat
     4981 gcgcccggtt cccgggcgct catcgacccg ggtactccac gctgaccgtg atggccgcgc
     5041 acgcgatgat ggtgtcggct cccggcacct tgaggccgcc cgcgacggga cgcaccgtca
     5101 cctcgccgtg gggcagctcc ttcttcacgc gctccacgtc gatcttgccg ggat
//
LOCUS       DATMCV010000278         2300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_547533, whole genome shotgun sequence.
ACCESSION   DATMCV010000278 DATMCV010000000
VERSION     DATMCV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547533"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1098)
                     /locus_tag="VNU03_06450"
     CDS             complement(115..1098)
                     /locus_tag="VNU03_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWP74133.1"
                     /translation="MTKRERVLSAARRGPVDRPPVSFWRHVPDVDHTAAGLAEAMLTF
                     QRKWDLDFVKVMSSGVYCVEDWGCRVAYQGSPNGAKQCTEHAVRSRADWARIAPLDEG
                     AGALGRELEAVRLIARGRADDAPILHTLFSPLTVARKLAGDRLVEDLRAQPAAVEPAL
                     DAITDTMARHARAALEAGADGFFFATQTAAPDVVTVEEAARFELPRMRRILDAIAGRS
                     ALTLLHVHGRDVYFDQAAALPVHAMNWHDRLATPTLGDGHRRFAGAVVGGLSEHRTLQ
                     AGPPDAVAAEVRDAIAQTGGRGVIIGPGCVLPLATPDAHLAAVVVTVKAAT"
     gene            1206..1763
                     /locus_tag="VNU03_06455"
     CDS             1206..1763
                     /locus_tag="VNU03_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP74134.1"
                     /translation="MREAEAGDAPRFLDRLRAGETAAFEELVITYQHRVFGVAVRMLG
                     NAAEAQEVAQEAFLRAHRGLAEFRGDAKLSTWLYAITSRLCLNRLATAERRLHRQGEE
                     TLLRLAGGEWPDVAVERSEVEAALHRAIAELPEERRIVVVLRDLEGLSYEEIAEALEL
                     ELGTVRSRLHRARADLKNKLERFLP"
     gene            1760..>2300
                     /locus_tag="VNU03_06460"
     CDS             1760..>2300
                     /locus_tag="VNU03_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWP74135.1"
                     /translation="MTHDDARESLSAFLDEELPLEERREVAAHIEGCPECRRELAHLE
                     QTVALLRRVEPARAPVGFVDRVLEAARPRPWYRRAAAAVFLPFSVKLPAEATALVMVA
                     LLAVYVFERTPALQESARPQPGVREIPREKTARPAPPTARTDRFARDSSIGERSPESK
                     ARPVEKDQDRREVPAAREKI"
BASE COUNT          293 a          854 c          856 g          297 t
ORIGIN      
        1 cccggatctc gcccgccgcc tgggcccgcg ccggagcggg cgcggtcccg atcgcggcga
       61 ccgcgagcgc ggccagcgcc aggacgctcg ccacgccggg cgcgcggcgg cggctcatgt
      121 cgccgccttc accgtcacga ccaccgcggc caggtgcgcg tccggcgtcg cgagcggaag
      181 cacgcagccg ggcccgatga tcaccccgcg ccccccggtc tgcgcgatcg cgtcgcgcac
      241 ctcggcggcc accgcgtcgg gcgggccggc ctggagcgtc cggtgctcgc tgaggccgcc
      301 caccactgct cccgcgaagc gccggtggcc gtctccgagc gtgggcgtgg ccagccggtc
      361 gtgccagttc atcgcgtgga ccggcagggc cgccgcctgg tcgaagtaga cgtcgcgccc
      421 gtgcacgtgc aggagggtca gggcggagcg cccggcgatc gcgtccagga tgcgccgcat
      481 gcgcggcagc tcgaaccgcg cggcctcctc gacggtgacg acgtcgggcg cggcggtctg
      541 ggtggcgaag aaaaagccgt cggcgcccgc ttcgagcgcg gcgcgcgcgt ggcgggccat
      601 ggtgtcggtg atggcgtcga gcgccggctc gaccgcggcg ggctgggcgc gcagatcctc
      661 gaccagccgg tcccccgcga gcttgcgcgc caccgtgagc ggggagaaga gcgtgtgcag
      721 gatgggcgcg tcgtcggcgc gcccccgcgc gatcaggcgc accgcctcga gctcgcggcc
      781 gagcgcgccg gcaccctcgt cgagaggcgc gatgcgggcc cagtcggctc ggctccgcac
      841 cgcgtgctcg gtgcactgct tggctccgtt gggcgagccc tggtacgcca cccggcagcc
      901 ccagtcctcc acgcagtaca cgccgctcga catcaccttg acgaagtcca ggtcccactt
      961 ccgctggaag gtcagcatgg cctcagccag cccggccgcg gtgtggtcga cgtcgggcac
     1021 gtgacgccag aaggacacgg ggggacgatc caccggccct cgacgggccg ccgacaggac
     1081 acgctcgcgc ttggtcatga tgccgcggag tctaccatgc cgatccgcgc ggctcgggag
     1141 gggccgacgt cattgctcac cgctttcgtt acgcgggaac ttccccgccg cggtatggtc
     1201 ttaccgtgcg agaggcggag gcaggcgacg cgccgcgctt ccttgaccgg ctgcgtgccg
     1261 gcgagactgc cgccttcgag gagctggtga tcacgtacca gcatcgcgtg ttcggggttg
     1321 cggtccgtat gctgggcaac gcggccgagg cccaggaggt ggcccaggag gccttcctcc
     1381 gcgcccaccg cggcctggcc gagttccgcg gggacgccaa gctctcgacc tggctctacg
     1441 ccatcacctc gcgcctctgc ctcaaccggc tcgcgaccgc cgagcggcgg ctgcaccggc
     1501 agggcgagga gacgctgctg cggctggccg gcggcgaatg gccggacgtc gcggtcgagc
     1561 gcagcgaggt cgaggcggcg ctccaccgcg ccatcgcgga gctgcccgag gagcgccgca
     1621 tcgtggtggt gctgcgtgac ctcgagggcc tctcgtacga ggagatcgcc gaagcgctcg
     1681 agctcgagct ggggacggtc cgctcgcgcc tgcaccgggc gcgcgcggac ctgaagaaca
     1741 agctggagcg ctttctccca tgacccacga cgacgcgcgc gaatcgctct ccgccttcct
     1801 ggacgaggag ctccctctgg aggaacgccg agaggtggcc gcccacatcg agggctgccc
     1861 ggagtgccgg cgcgagctgg cgcacctcga gcagacggtg gccctgctcc ggcgggtcga
     1921 gcccgcccgc gcgccggtgg gcttcgtgga tcgcgtcctc gaggctgcgc ggccccggcc
     1981 ctggtaccgc cgcgccgccg ccgcggtgtt cctgcccttc tcggtgaagc tgcccgccga
     2041 ggccaccgcg ctcgtgatgg tcgcgctgct cgcggtgtac gtgttcgagc gcacgcccgc
     2101 gcttcaggag tcggcccgac cccagcccgg ggtccgagag attccccgtg agaagacggc
     2161 gcggcccgcg ccgccgacgg cacggaccga ccgcttcgcg cgtgactcgt ccatcggaga
     2221 gcgctccccg gaaagcaagg cgcgtccggt cgagaaggac caggaccggc gcgaggttcc
     2281 ggccgcgcgc gagaagatcg
//
LOCUS       DATMCV010000279         4944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_481334, whole genome shotgun sequence.
ACCESSION   DATMCV010000279 DATMCV010000000
VERSION     DATMCV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481334"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..3524)
                     /locus_tag="VNU03_06465"
     CDS             complement(156..3524)
                     /locus_tag="VNU03_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP74136.1"
                     /translation="MSPGSLACAHCQTPNPPSHRYCVECGQALLVACPRCGFAPEAGA
                     RFCGGCGHAIAAPAPIPDRFASPDVYTPPDLAEKIRTARGAVEGERKQVTVLFADMKG
                     SLELLADRDPEDARRMLDPVLALMMEAVHRYEGTVNQVLGDGIMAIFGAPVAHENHVV
                     RAAYAALRMHEGVRGLARELRRAEGLDVQIRIGLNSGEVVVRSIGSDLRMEYTAVGQT
                     TYLAAQMERLARPGATLLTAESRRLAEGYIAVTPLGPVPVRGAAEPIEVYELTGPGPA
                     RYRLQAATVRGLSRFVGRDEEVTRLRQALELARAGHGRMVALVGEPGVGKSRVIREFT
                     EGPWIAGALVLEGRPIVYRRTPSWLPVIEALRRYFQVEPRDDARVIREKIAAKVVALD
                     PALEPTVPALHALADLEPDDPAWRQLDPPQRRRRIQDAVRNLLLAETRRQPVVLIVED
                     LQRIDEETQALLDGLVDRLPGTRLLILVAYRPEYQHAWGTRPGYSQIRVDPFVPETAR
                     VLLETLLGPDRDLEPLKALLVSRTDGNPLFLEESVRALVETQALVGDPGSRRLVKALE
                     TVQVPGSVQAVLAARIDRLSALAKHVLQSASVIGKDVSLPVLEAIADLPGEALRAALG
                     QLQAGELLHEASFFPEVEYTFKHALTHEVAYGSLLHETRRALHARIVEVTEALYPERL
                     GEHVERLAFHALRGGLRDRAVDFLRQAGLRAAARSAHREAVSFFEQALEILEADGPRE
                     GTLPAAIDLVFDLRASLVPLGEFTRTLAHLRRAEERAETLGDRWRLGWVSAYLTQSYY
                     TLGEQAAAIRSAERALAIGRELGDRPLQIAASFGLGQAHHVLGAYPAAQRHLRSAVAA
                     VDGELVRERWGMAGLVSVAARVWLAASLAEVGEFGEALECMHAGLAVAEAADHPWSIA
                     GSLMTVGFAHLSRGHLEAALPVVDRGIAFSREMDLTAWLPMLLCVRGAADVRGGRVAE
                     GVLLLEEGVGRAASLRILSRQALRLTWLAEGYRLAGRVEEAVTTAREAHRLASEQEEQ
                     GGAAGALRMVGEALAPADPASAARHGESALAAAQALGMRPLEALCRLDLGALAARAGR
                     RDEARDHLETAVRMLRAMDMRHWLPGAEGALAAL"
     gene            complement(3529..3957)
                     /locus_tag="VNU03_06470"
     CDS             complement(3529..3957)
                     /locus_tag="VNU03_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74137.1"
                     /translation="MSRPDPAELGRRLDEILAAAERLIRPVPEAEMESLRDPAFQLFR
                     LGLGFVDGMDRGRLAGDWRHERAPEDLRDGGSVARYGALVRARVGGWFEGAGPRELDR
                     VIDGPEGPRPGRALLEQVTHEAAAQLRLLQAALDRSGRVS"
     gene            complement(3978..4766)
                     /locus_tag="VNU03_06475"
     CDS             complement(3978..4766)
                     /locus_tag="VNU03_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWP74138.1"
                     /translation="MGYETLRYEVKEGVGTITLNRPEAYNALNLALGRDLFHAALEAD
                     EDRAVRCIVITGAGKAFCAGGDVKDFADNPDRIGALVKELTTYLHGAISRLARAPKPV
                     VMAVNGIAAGGGMSLALSGDLVVAAESTKFTMAYSKIAASPDGSSSYFLPRMIGLRRA
                     LELHYTNRVLSAREAMEWGLVNRVHPDAEFPGAVAGLARELAQGPTLAFGRAKLLFHQ
                     STQESLETQMELEAQAIAASGHTEDFRNGVVAFARKQPVTFAGR"
BASE COUNT          656 a         1910 c         1770 g          608 t
ORIGIN      
        1 ctcccgcgcg gccacgcagt gggacccccg agtccggtgc gccgctcgaa gagcggcgag
       61 tccggcgagt gggtgacgga gacggccgcg cgggaggcgc ccacgccacc aggacccgtc
      121 cgcctctcgc gatgaccgtc cccgagtagc tcgggctaga gcgcggccag ggccccctcc
      181 gcgccgggca gccagtgccg catgtccatg gcgcggagca ttcgcaccgc ggtctccagg
      241 tggtcacgcg cctcatcgcg ccggcccgcg cgcgcggcca gcgcgcccag atcgaggcgg
      301 cagagagcct cgagcgggcg catgccgagg gcctgcgccg ccgcgagcgc gctctcgcca
      361 tgccgcgcgg ccgaggccgg atccgcgggc gcgagcgctt cgcccaccat gcgaagcgct
      421 cccgccgcgc caccctgctc ctcctgctcg gaggcgagcc gatgcgcttc ccgcgcggtg
      481 gtcaccgcct cctccacgcg cccggccagg cggtagccct cggcgagcca agtgaggcgg
      541 agcgcctggc ggctcaggat ccggaggctc gccgcgcgcc cgacgccctc ctcgagcagc
      601 agcacgcctt cggccacgcg cccgccgcgg acatcggccg cgccgcgcac gcagagcagc
      661 atgggcagcc aggccgtgag gtccatctcg cgcgagaagg cgatgccgcg atcgaccacc
      721 ggcagcgcgg cctccaggtg gccgcggctc aggtgcgcga agcccaccgt catcaggctc
      781 cccgcaatgc tccacggatg atccgccgcc tctgcgacgg cgaggccggc gtgcatgcac
      841 tcgagcgcct cgccgaactc gccgacctcg gccagcgaag cggcgagcca gacccgcgcg
      901 gccaccgaca cgaggccggc catgccccag cgctcccgga ccagctcgcc gtcgaccgcg
      961 gccaccgcgc tgcgcaggtg gcgctgggcc gccgggtagg ccccgagcac gtggtgagcc
     1021 tggccgagcc cgaaggacgc cgcgatctgc agcggccgat caccgagctc cctcccgatc
     1081 gcgagcgcgc gctccgccga gcggatggcc gcggcctgct cgccgagcgt gtagtacgac
     1141 tgggtcaggt acgcggagac ccacccgagc cgccagcggt cgccgagggt ctcggcgcgc
     1201 tcttccgcgc gccgcaggtg cgccagcgtg cgggtgaact cccccagcgg gacgagcgag
     1261 gcgcgcaggt cgaagaccag gtcgatcgcg gcggggagcg tcccctcgcg cgggccgtcg
     1321 gcctcgagga tctcgagcgc ctgctcgaag aagctcaccg cctcgcggtg cgccgagcgc
     1381 gcggcggccc ggagcccggc ctggcgcaga aagtccaccg cccggtcgcg caggccgccg
     1441 cggagcgcgt ggaaggccag gcgctcgacg tgctcaccca gacgttccgg gtagagggcc
     1501 tccgtcacct cgacgatccg cgcgtgcagc gcccgtcggg tctcgtgcag caggctgccg
     1561 taggcgacct cgtgcgtgag cgcgtgcttg aacgtgtact cgacctccgg gaagaagctc
     1621 gcctcgtgga gcagctcgcc ggcctggagc tggccgaggg ccgcccgcag ggcctccccg
     1681 ggcaggtccg cgatggcctc cagcaccggc agggacacgt ccttgccgat caccgaggcg
     1741 gactgcagca cgtgcttggc cagggcgctc aggcggtcga tgcgggccgc cagcaccgcc
     1801 tgcacgctgc ccggcacctg tacggtctcg agcgccttca cgagccgccg cgagcccggg
     1861 tcgcccacca gggcctgcgt ctccaccagc gcccgcacgc tctcctccag gaagagcggg
     1921 ttcccgtcgg ttcgcgacac cagcagcgcc ttgaggggct ccaggtcgcg atcgggtccg
     1981 aggagggtct cgagcagcac gcgggcggtc tcgggcacga agggatcgac ccggatctgg
     2041 gagtaacccg gccgggtgcc ccacgcgtgc tggtactcgg gccggtacgc gaccaggatc
     2101 aggagccgag tccccgggag ccggtccacc aggccgtcga gcagggcctg cgtctcctcg
     2161 tcgatgcgct gcaggtcctc gacgatcagc accaccggct gccggcgcgt ctcggcgagc
     2221 agcaggttgc ggaccgcgtc ctggatgcgg cggcggcgct gcggcggatc gagctgccgc
     2281 cacgcgggat cgtccggctc gaggtcggcc agggcgtgga gggccgggac ggtgggctcg
     2341 agcgccgggt cgagcgccac gaccttggcc gcgatcttct cgcggatcac ccgcgcgtcg
     2401 tcccgcggct ccacctggaa gtagcgccgg agcgcctcga tcaccggcag ccacgacggg
     2461 gttcgccggt agacgatcgg ccggccctcg agcaccagcg cgcccgcgat ccacggccct
     2521 tccgtgaact cgcggatgac ccgcgacttg ccgaccccgg gctcgccgac cagggccacc
     2581 atgcgcccgt gtccggcgcg ggcgagctcg agcgcctggc ggagccgggt cacctcctcg
     2641 tcgcggccca cgaagcggga gagcccgcgc acggtggcgg cctgcaggcg gtagcgggcc
     2701 ggccccggcc cggtcagctc gtacacctcg atcggctccg cggcgccccg caccggcacc
     2761 gggccgaggg gcgtcaccgc gatgtagccc tcggccaggc gccggctctc ggcggtgagg
     2821 agcgtcgcac cgggacgggc gagccgctcc atctgcgcgg ccaggtacgt cgtctggccg
     2881 accgcggtgt actccatgcg caggtcgctg ccgatcgagc gcaccaccac ctcgcccgag
     2941 ttgagcccga tgcggatctg cacgtcgagc ccctcggcgc ggcgcagctc gcgggccagg
     3001 ccgcgcacgc cctcgtgcat gcgcagcgcc gcgtaggccg cgcgcaccac gtggttctcg
     3061 tgggcgaccg gggcgccgaa gatcgccatg atcccgtcgc ccagcacctg gttgacggtg
     3121 ccctcgtacc ggtgcaccgc ctccatcatc agggccagca ccggatccag catgcgccgc
     3181 gcgtcctcgg gatcgcggtc ggccagcagc tcgagcgagc ccttcatgtc cgcgaagagc
     3241 acggtcacct gcttgcgctc gccctccact gccccgcgcg cggtccggat cttctcggcc
     3301 aggtccggcg gggtatagac gtcgggggag gcgaagcgat ccgggatcgg ggcgggcgcc
     3361 gcgatcgcat ggccgcaccc gccgcagaag cgcgcgcccg cctcgggcgc gaagccgcag
     3421 cgggggcatg ccaccaggag cgcctgcccg cactccacgc agtagcgatg gctgggcggg
     3481 ttcggggtct gacagtgggc gcaggcgagc gatcccggac tcacgcggct acgacacccg
     3541 gccggagcga tcgagcgcgg cctggagcag gcgcagctgc gcggccgcct cgtgggtgac
     3601 ctgctcgagc agcgcccgtc ccggccgcgg cccctcgggc ccgtcgatga cccggtcgag
     3661 ctcccggggc cccgcgcctt cgaaccagcc ccccacccgc gcccgcacga gcgcgccgta
     3721 ccgcgcgacc gagccgccgt cgcgcagatc ctcgggcgcg cgctcgtgcc gccagtcgcc
     3781 cgccagccgg ccgcggtcca tgccgtcgac gaagcccagc ccgaggcgga agagctggaa
     3841 cgcggggtcg cgaaggcttt ccatctccgc ctcgggcacc gggcggatca atcgctccgc
     3901 cgcggccagg atctcgtcca gccggcgccc gagctccgcg ggatccggtc ggctcacgcg
     3961 gtcacgcggc gcgacgctca gcggcccgcg aacgtgaccg gctgcttgcg cgcgaacgcg
     4021 acgaccccgt tgcggaagtc ctcggtgtgg ccgctggccg cgatggcctg cgcctccagc
     4081 tccatctgcg tctcgaggct ctcctgggtc gactggtgga agagcagctt ggcgcggccg
     4141 aaggcgagcg tcggcccctg cgcgagctcg cgggcgagcc ccgcgaccgc ccccgggaac
     4201 tccgcgtcgg ggtgcacccg gttgaccagg ccccactcca tcgcctcccg ggccgagagc
     4261 acccggttgg tgtagtgcag ctcgagcgcg cggcggaggc cgatcatgcg cggcaggaag
     4321 taggacgacg agccgtccgg cgaggccgcg atcttcgagt aggccatggt gaacttcgtc
     4381 gactccgcgg ccaccaccag gtcgccggag agagccaggc tcatgccccc gcccgccgcg
     4441 atcccgttga ccgccatcac caccggcttg ggcgcgcggg ccagccgcga gatcgcgccg
     4501 tgcaggtagg tcgtcagctc cttgaccagg gcgccgatgc ggtccggatt gtccgcgaag
     4561 tccttcacgt cgccgcccgc gcagaacgcc ttgcccgccc cggtgatcac gatgcagcgc
     4621 accgcccgat cctcgtcggc ctcgagcgca gcgtggaaca gatcccgccc cagcgccaga
     4681 ttcagcgcgt tgtacgcctc cggccgattc aacgtgatcg tcccaacccc ctccttcacc
     4741 tcataccgca gcgtctcata gcccatgtct ctctccagtc gtggggatcg tcggtgatcc
     4801 cgccagcggg atcgacccgg gcggggtggg gccggggggg tgccctcccc gggaccgtgg
     4861 cttctgcggg ttatcgaagt cgtctacatc ccatctcgtg gctcgacaat acgcgcgacg
     4921 cgctgcgagt cagcgtgtcc cggg
//
LOCUS       DATMCV010000280         7090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_949246, whole genome shotgun sequence.
ACCESSION   DATMCV010000280 DATMCV010000000
VERSION     DATMCV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949246"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VNU03_06480"
     CDS             <1..597
                     /locus_tag="VNU03_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP74139.1"
                     /translation="AAIGSGPTGKARLDEAEEHLRRAIALRPDSPIPYFNLGNVLLVR
                     KQYPDAELAFRRYGDLSPGSPQGLARLGLLAILRGDYAEAVSLLARARGAPAGLDPAP
                     GTPARLLVVAVDLVEDDAGTLTLLGQVLVEQGHPSEAVGALRRAAALDPANAPARLTL
                     IQAYRDSGHRDLARQEMEKLGSLAPATPGGALRPLTAR"
     gene            661..1851
                     /locus_tag="VNU03_06485"
     CDS             661..1851
                     /locus_tag="VNU03_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWP74140.1"
                     /translation="MRMPRLRLVITSIAALALVLPVTSVADAQSKGTIKIATQSPLSG
                     GQAVLGEGIKLGAQLAVEKMKGNLEKMGYKVDFVPFDDQAKPDVGVANAKNIIADKDI
                     MAIIGHLNSGVAIPSSEVYKEVSLAMISPANTNPVVTDRNYPNVNRVCGRDDVQGVVG
                     SEFAHGTLKIKSAYIVHDKTQYGQSIAEFFKADAEKKGVKVVGFEGTEEKSNFDPLLT
                     PIKAKNPDLIYFGGIYDQGAPFFKQAREKGIKSKFMGPDGMDSSDLTKIAGKAVVGMN
                     YTSAAGPASALPKAKAFVDDFKKKFSKNPEPYAAEAYDATVMAIKAIEDVAKTGKMSR
                     EAVSAAVRKVKLSGITGEIAFDGKGDRLKAQYFVLSVASDNPEKWGDNKIIKQLTIAP
                     PAAK"
     gene            1924..2895
                     /locus_tag="VNU03_06490"
     CDS             1924..2895
                     /locus_tag="VNU03_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74141.1"
                     /translation="MDFEILIGIFPQVLIDGITLGFMYALIALGYTMVYGVLEFINFA
                     HSEIFIIGAFVGVEILLSFKAAGWLDVLPWALVLLIILVAGMLVSGVVAVVIERTAYR
                     PLRHAPRLIPLISAIGVSFFLQDVIRLFESLWRNSFNLVYPTMEVLNHRFELTDTIDV
                     SVKSLVVIVAALLMLWGLHTLVNRTKVGKAIRAVAEDQAAASLMGINVNRIISLTFLI
                     GGAMGGGAGVLFGVQYSLINPYTGFIPGLKAFTAAVLGGIGNVPGAMLGGLVLGLLEA
                     FAASYLSLLTGGAFGAEYKDIFAFSILILILIFRPKGLLGEIVRERA"
     gene            2892..4157
                     /locus_tag="VNU03_06495"
     CDS             2892..4157
                     /locus_tag="VNU03_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74142.1"
                     /translation="MIKDLLDRPGPATALVVALLLVTTWGVTYSPRSIVLFMLFQGSI
                     LLLYFARIPTPLKAALTILTLGVLMPLLGTINAYYMEIAIQVGIFVALALGLNIVVGL
                     AGLLDLGYVAFFAVGAYSWAIFGSPQANLIFGGEHFPLGPWWFFVFLFVGVAVAAGAG
                     ILLGLPVLRLHGDYLALVTLGFGEVIRVLANNLDKPINFTNGPKGITPINRPPIFFAP
                     LLRSLGIDPDPNVIYPLYLYALVLLIVGATVLANRRLEDSHIGRAWEAIREDQTAAQA
                     MGVPLVRMKLLAFGCGASFAGTVGVLFSAKQIFINPESFTFMESIGVLAMIILGGMGS
                     IPGAILGASVVTILNLQILKGLSLWLNELRNAGTVILGYDLTNLPTQLEPAKYERMIF
                     GLILVLMMIFRPQGILPAKRRHRELEEER"
     gene            4157..4960
                     /locus_tag="VNU03_06500"
     CDS             4157..4960
                     /locus_tag="VNU03_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74143.1"
                     /translation="MALLEARGVSKRFGGLTALNAVDFTLEEGHIASIIGPNGAGKTT
                     FFNVFTGIYVPEEGVVRFRDTVVQGRRPDQITELGICRTFQNIRLFSRMTAIENVLVG
                     MHSRVPLGFWDVISRNARWRAHEQRQWEQGAELLAVAGLRDKANELARNLPYGEQRRL
                     ELARALASNPSLLLLDEPTAGMTQGEARSLMALLRRLVEERHLTLLLIEHNMRVVMEV
                     SDHVTVLDYGEKIAEGRPAEVQRNPRVIEAYLGRRRWTPGSGTAGSAHA"
     gene            4953..5657
                     /locus_tag="VNU03_06505"
     CDS             4953..5657
                     /locus_tag="VNU03_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74144.1"
                     /translation="MLEVTDLHVYYGQIHALKGISFRVGQGEIVTLLGNNGAGKTTTL
                     RTLSGLLAPRQGDVRFEGTSLLGIAAHDVVLKGITHVPEGRRIFNRLSVVENLEMGAY
                     TRSDAAIAEDMDRVFTTFPRLKERRTQVAGTLSGGEQQMLAIGRALMARPRLLLLDEP
                     SMGLAPVLVEQIFETVLAINQQGVTVLLVEQNAAMALSIAERGYVLETGRLPLEGAAR
                     ELADNPEVRRAYLGEA"
     gene            5672..6127
                     /locus_tag="VNU03_06510"
     CDS             5672..6127
                     /locus_tag="VNU03_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWP74145.1"
                     /translation="MKTRGLTYEEHAVGAVYQTLGRTVSEADICAFVNLCGFNEPLFM
                     DMEYVARESVFKGRAAPGAMTFALSEGLIMQTGLIHGTGMSYLGGEIRIVSPVLEGDT
                     LHVSVTITDKRETKKPDRGIVTYRHEVTNQRGEIVLEATVKRMIKRVAG"
     gene            6368..>7090
                     /locus_tag="VNU03_06515"
     CDS             6368..>7090
                     /locus_tag="VNU03_06515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP74146.1"
                     /translation="MRTADAIIIGGGVIGASIAFHLAGLGVRRVLVLERSALASGGTG
                     YSVGIIRQLYPTRETSEMVLRSLAVFENFGAAVGGDSGFIRCGALIGVAPAMRSTLER
                     TLVLQRDIGIEAEIVEPDALARIEPRIDPSGLGAVLWEPRSGYGDPTAVTAGFAGAAR
                     RRGAVIEQGVEVVAVRREGHRVTGVDTKAGDQIDAPIVVDAAGLWAPHVATLASIELP
                     IVVGRHPVFIVERDAAFGPAHPV"
BASE COUNT         1128 a         2504 c         2319 g         1139 t
ORIGIN      
        1 gcggccatcg gcagcggccc gaccggaaag gcccgcctcg acgaagcgga ggagcacctg
       61 cgccgagcca tcgctctccg tccagacagc ccgatcccct acttcaacct cggcaacgtc
      121 ctgctcgtcc ggaagcagta cccggacgcg gagctcgcgt tccggagata cggtgacctc
      181 tctcccggct cgccccaggg cctggctcga ctcgggctcc tcgccatcct gcgcggcgac
      241 tacgcggagg cggtgtccct tctggcgagg gcccggggcg ctccggccgg gctcgacccc
      301 gctcccggca cgccggccag gctcctggtg gtggcggtcg acctcgtgga ggacgatgcc
      361 gggaccctca ccctgctggg gcaggtcctc gtcgagcagg gtcacccatc cgaggcggtg
      421 ggggcgctcc ggcgggcagc cgcgctggac cccgcgaacg cgccggcccg cctcacgctg
      481 atccaggcgt atcgggacag tgggcaccgg gacctcgcgc ggcaggagat ggagaagctc
      541 gggagcctgg cccccgcaac ccccggaggg gctctccgtc cgttgaccgc tcggtagaaa
      601 ccgctataat ggccgcgctc gaacctaccc gttcgccctg gtcacaagga ggaggagccg
      661 atgagaatgc cgcgactacg cctggtcatc acctcgattg ctgcattggc gctggtgctg
      721 ccggtgacct ccgtcgccga cgcccagtcc aagggcacca tcaagatcgc cacccagagc
      781 ccgctctcgg gcggccaggc cgtactcggg gaaggcatca agctcggcgc ccagctggcg
      841 gtcgagaaga tgaagggcaa cctggagaag atgggctaca aggtcgactt cgtgccgttc
      901 gacgaccagg ccaagcccga cgtcggcgtc gccaacgcca agaacatcat cgccgacaag
      961 gacatcatgg cgatcatcgg gcacctgaac tcgggagtcg ccatcccctc ctccgaggtc
     1021 tacaaggaag tctcgctggc gatgatctcc cccgccaaca ccaacccggt ggtcaccgac
     1081 cgcaactacc cgaacgtcaa ccgggtgtgc gggcgtgacg acgtgcaggg cgtggtgggc
     1141 tccgagttcg cgcacggtac cctgaagatc aagagcgcgt acatcgtcca cgacaagacc
     1201 cagtacgggc agagcatcgc cgagttcttc aaggcggacg ccgagaagaa gggggtcaag
     1261 gtggtgggct tcgagggtac cgaggagaag tcgaacttcg acccgctcct gaccccgatc
     1321 aaggccaaga accccgacct catctacttc ggcgggatct acgaccaggg cgcgccgttc
     1381 ttcaagcagg cgcgcgagaa gggcatcaag tccaagttca tgggcccgga cggcatggac
     1441 tcctcggacc tgaccaagat cgcgggcaag gcggtggtgg gcatgaacta cacctccgcg
     1501 gcgggcccgg cctccgcgct gcccaaggcc aaggccttcg tcgacgactt caagaagaag
     1561 ttcagcaaga accccgagcc ctacgcggcc gaggcctatg acgcgacggt catggcgatc
     1621 aaggccatcg aggacgtcgc caagaccggc aagatgagcc gggaggcggt gtcggccgcg
     1681 gtgcgcaagg tcaagctttc cggcatcacc ggcgagatcg ccttcgacgg caagggcgac
     1741 cggctgaagg cgcagtactt cgtgctgtcg gtggcgagcg acaacccgga gaagtggggc
     1801 gacaacaaga tcatcaagca gctcacgatc gcgccccccg cggccaagta gccgccacgg
     1861 tcatcggcgg gaggggcgga gtcgcgcccc tcccgcccac ccccgtcttc ccgcccgccg
     1921 ccgatggact ttgagatcct gatcggcatc ttcccgcagg tcctgatcga cggcatcacc
     1981 ctgggcttca tgtacgccct gatcgcgctc ggctacacca tggtctacgg cgtgctcgag
     2041 ttcatcaact tcgcccactc cgagatcttc atcatcgggg ccttcgtcgg cgtcgagata
     2101 ctcctctcct ttaaggcggc gggctggctc gacgtgctgc cgtgggcgct cgtgctcctc
     2161 atcatcctgg tggcggggat gctggtgagc ggggtggtag cggtggtgat cgagcgcacc
     2221 gcctaccggc cgctgcgcca cgcgccccgg ctcatccccc tgatctccgc gatcggggtc
     2281 tcgttcttcc tccaggacgt gatccggctc ttcgaatcgc tctggcgcaa ctccttcaat
     2341 ctcgtatacc ccaccatgga ggtgctgaac caccggttcg agctgaccga caccatcgac
     2401 gtctcggtca agtcgctggt ggtcatcgtc gcggccctgc tcatgctctg gggcctgcac
     2461 accctggtta accggaccaa ggtgggcaag gcgatccggg cggtcgcgga ggaccaggcc
     2521 gccgcgtcgc tcatgggcat caacgtgaac cggatcatct cgctgacctt cctcatcggc
     2581 ggggcgatgg gcgggggcgc gggagtgctc ttcggcgtcc agtacagcct catcaacccg
     2641 tataccggct tcattcccgg gctcaaggcc ttcaccgccg cggtcctcgg cggcatcggc
     2701 aatgtgccgg gagcgatgct gggcgggctc gtgctggggc tgctcgaggc cttcgcggcc
     2761 tcctatctgt cgctgctcac cggcggcgcc ttcggagccg agtacaagga catcttcgcg
     2821 ttctcgatcc tcatcctgat cctcatcttc aggccgaagg gcctcctggg agagatcgtc
     2881 cgcgagcggg catgatcaag gacctcctcg atcgtccggg gcccgcgacc gcgctagtgg
     2941 tcgcgctcct gctggtcacc acctggggcg tgacctacag tccccgctcg atcgtgctct
     3001 tcatgctgtt ccagggctcg atcctcctgc tctacttcgc ccggatcccg accccgctga
     3061 aggcggccct caccatcctc accctcggcg tgctcatgcc gctcctcggc acgatcaacg
     3121 cgtactacat ggagatcgcg atccaggtcg ggatcttcgt ggccctggcc ctcgggctca
     3181 acatcgtggt ggggctggcc ggtctgctgg atctgggcta cgtcgccttc ttcgcggtcg
     3241 gcgcctactc ctgggcgatc ttcggctcgc cccaggcgaa cctgatcttc ggcggagagc
     3301 acttcccgct cggcccgtgg tggttcttcg tgttcctctt cgtgggagtg gcggtggcgg
     3361 cgggggcggg catcctgctc ggccttccgg tgctgcggct ccacggcgac tacctggccc
     3421 tggtgaccct cggattcggc gaggtgatcc gcgtgctcgc caacaacctc gacaagccga
     3481 tcaacttcac caatggcccc aagggcatca cgccgatcaa ccggccgccg atcttcttcg
     3541 ccccgcttct ccgatcgctg gggatcgacc ccgaccccaa cgtgatctac ccgctctatc
     3601 tctacgcgct cgtgctgctg atcgtgggcg cgacggtgct cgcgaaccga cgtctggagg
     3661 actcccacat cgggcgcgcc tgggaagcga tccgcgagga ccagaccgcg gcgcaggcca
     3721 tgggcgtgcc cctggtgcgg atgaagctcc tcgccttcgg gtgcggggcc tcgttcgcgg
     3781 ggacggtagg cgtgctcttc tcggccaagc agatcttcat caaccccgag tccttcacct
     3841 tcatggagtc gatcggcgtc ctggccatga tcatcctggg cggcatgggc tccatccccg
     3901 gcgccatcct gggggcctcg gtcgtgacca tcctgaatct ccagatcctg aagggcctct
     3961 ccctctggct caacgagctg cgcaacgcgg gtaccgtgat cctcggctac gatctcacca
     4021 acctgcccac ccagctcgag ccggccaagt acgagcggat gatcttcggg ctcatcctgg
     4081 tcctcatgat gatattccgg ccccagggca tcctcccggc caagcgccgc catcgcgagc
     4141 tcgaggagga gcgctgatgg cgctgctgga ggcccgcggg gtgagcaagc ggttcggcgg
     4201 cctgaccgcg ctcaacgcgg tggacttcac cctcgaggaa gggcacatcg cctcgatcat
     4261 cgggcccaac ggagcgggca agaccacctt cttcaacgtc ttcaccggca tctacgtgcc
     4321 ggaggaaggc gtcgtccgct tccgcgacac cgtggtgcag ggccgccgcc ccgaccagat
     4381 caccgagctc ggcatctgcc ggaccttcca gaacatccgc ctgttctccc gcatgaccgc
     4441 catcgagaac gtgctggtcg ggatgcacag ccgggtgccg ctcggcttct gggacgtgat
     4501 cagccggaac gcgcgctggc gcgcccacga gcagcggcag tgggagcagg gcgcggagct
     4561 gctcgcggtg gccgggctgc gcgacaaggc gaacgagctg gcccgcaacc tgccctacgg
     4621 cgagcagcgg cgcctcgagc tggcccgggc gctcgcctcg aatccctcgc tgctgctcct
     4681 ggacgagccg accgcgggca tgacccaggg cgaggcccgg agcctgatgg ccctgctgcg
     4741 gcgcctcgtc gaggagcgcc acctcaccct cctgctcatc gagcacaaca tgcgggtggt
     4801 catggaggtc tccgaccacg tcaccgtcct ggactacgga gagaagatcg cagagggtcg
     4861 gcccgccgag gtgcagcgga atccccgcgt gatcgaggcc tacctgggcc gtcgccgttg
     4921 gacccccggg tcgggcacgg ccgggagcgc gcatgcttga ggtgaccgat ctccacgtct
     4981 actacggcca gatccacgcc ctgaagggca tctcctttcg ggtgggccag ggcgagatcg
     5041 tcaccctgct cggcaacaac ggcgccggca agaccaccac cctgcgtacg ctctccggcc
     5101 tcctcgcccc gcggcaaggc gacgtgcggt tcgaggggac ctcgcttctc ggcatcgcgg
     5161 cccacgacgt ggtgctcaag ggcatcaccc acgtgcccga ggggcgccgc atcttcaacc
     5221 gcctgagcgt ggtcgagaac ctcgagatgg gcgcctacac ccgcagcgat gcggccatcg
     5281 cggaggacat ggaccgcgtg ttcacgacct tcccgcgcct caaggagcgc cgcacccagg
     5341 tggcggggac cctctccggg ggcgagcagc agatgctcgc catcgggcgc gccctcatgg
     5401 cccggccccg gctcctcttg ctggacgagc cctcgatggg cctcgccccg gtgctcgtcg
     5461 agcagatctt cgagacggtg ctcgccatca accaacaggg ggtcaccgtc ctgctggtgg
     5521 aacagaacgc ggccatggcg ctctcgatcg ccgagcgcgg ctacgtgctg gagaccggcc
     5581 gcctgcccct ggagggcgcc gcccgcgagc tggccgacaa ccccgaagtg cgccgcgcct
     5641 atctgggcga ggcctgatcc cggaggagcg cgtgaagacg cgaggactca cgtacgagga
     5701 gcacgcggtc ggcgcggtgt accagacgct cggccgcacg gtctcggagg cggacatctg
     5761 cgccttcgtc aacctctgtg gcttcaacga gcccctgttc atggacatgg aatacgtggc
     5821 cagggagtcg gtgttcaagg gacgggccgc cccgggggcc atgaccttcg ccctctccga
     5881 ggggctcatc atgcagaccg ggctcatcca cggcaccggc atgtcctatc tcggcgggga
     5941 gatccgcatc gtctccccgg tcctggaggg cgacaccctc cacgtgagcg tcaccatcac
     6001 cgacaagcgc gagaccaaga agcccgatcg cggcatcgtc acctaccggc acgaggtgac
     6061 caaccagcgc ggcgagatcg tcctggaggc gaccgtgaag cggatgatca agcgcgtcgc
     6121 cggttagccg ggctggactc tcgtccagcg ccttcggggc cagaggaacc gggaccggcg
     6181 tagacgagga ttagccgaaa aataccggtg tggtggatac ttgaggggtg gcatccagcg
     6241 ataccccgag cggcagaccc cagatagtcg agatcagcgt ctcgtccctc tgcttgcccc
     6301 gcgactactc ccgggtgtcg cataggctgc tggcccagga cacgccccgc acgctcatct
     6361 cgccgcgatg agaaccgccg acgcgatcat catcggcggc ggggtgatcg gcgcctcgat
     6421 cgccttccac ctcgcgggcc tcggagtcag gcgcgtgctg gtcctcgagc ggagcgcgct
     6481 cgcgtccggc ggcaccggct attcggtcgg gatcatccgg cagctgtatc ccacccggga
     6541 gaccagtgag atggtcctcc gctcactcgc ggtcttcgag aacttcggcg cggcggtggg
     6601 aggcgactcc gggttcatca ggtgcggggc gctcatcggc gtcgctccgg cgatgcgatc
     6661 gaccctggag cggacgctcg tcctgcaacg cgacatcggg atcgaagccg agatcgtcga
     6721 acccgacgcg ctcgcgcgca tcgagcctcg catcgacccg tctggcctcg gggccgtcct
     6781 ctgggagccg cgctccggat acggagaccc gaccgccgtg accgccgggt tcgccggcgc
     6841 cgcgcgccgc cgcggcgccg tgatcgagca aggcgtcgag gtcgtcgccg tccgccggga
     6901 gggccaccgc gtcaccggcg tggacaccaa ggccggggac cagatcgacg cgccgatcgt
     6961 ggtcgacgcg gccggtctct gggcgccaca cgtcgccacg ctggccagca tcgagctgcc
     7021 gatcgtggtg ggacggcacc cggtgttcat cgtggagcgc gacgccgcct tcggccccgc
     7081 ccacccggtc
//
LOCUS       DATMCV010000281         3559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_149277, whole genome shotgun sequence.
ACCESSION   DATMCV010000281 DATMCV010000000
VERSION     DATMCV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149277"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VNU03_06520"
     CDS             complement(<1..990)
                     /locus_tag="VNU03_06520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP74147.1"
                     /translation="MADPVPAMNILMLGRWVGAPRYPLKATREYHFGRVLAQKHSLTL
                     AFTTDNPNAVGPISSLRSEFGDLEFTVVPRTWKSLASAVSLMTGESCTLSYFRSEALR
                     SRLADRIRTTPYDLVFVTSSSMIQYAMEVDPSIPVIVDFAHVDSEWWTRQASRGSFPG
                     SRFFRTEGVRLRATEALAARRAGHCLVATPEAAKLVRGFKPAGPVTLVADGVDVENFR
                     ARPRRGAPPTLVLATALGGASESHDALEFIRHVMPTVRSQYPEVRLVISSHESPRDAA
                     LTYRIPGVEVDAPRPDMRPALARASVAVAPLWTSDNMRQTVLQPMGGGIPVVVT"
     gene            complement(966..1988)
                     /locus_tag="VNU03_06525"
     CDS             complement(966..1988)
                     /locus_tag="VNU03_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HWP74148.1"
                     /translation="MDVRPLTTETVEWDAFVRSTPGGSPFHLIAWKRAVETAFRHRPH
                     YLLAFEGGAVAGVLPLFEVRGLFGGRGLVSVPYGVYGGVCAASASAASALVQAATELA
                     RRVKASYVELRHRPGRALDLPTKTLYVTFSRPISASEDENLAAIPRKQRRMTRQGLKH
                     GLRAELGLRHLDGFWDVYAHSVHALGSPVFPRRLFQAIAQEFGKECELLTIWKEETTV
                     AGVLSLFFEDQVLPYYGGALHEALPYAVNDFMYWELTCHAARIGSRSFDFGRSREGTG
                     AYHFKRHWGFEPVPLPYQYILLDGHPMPNLSPSNPKMRTAVDLWKRLPLPVTKVVGPW
                     LTRYLP"
     gene            complement(1994..3082)
                     /gene="wecB"
                     /locus_tag="VNU03_06530"
     CDS             complement(1994..3082)
                     /gene="wecB"
                     /locus_tag="VNU03_06530"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HWP74149.1"
                     /translation="MKILAVVGARPNFVKIAPIMAELREYPYLTTTLVHTGQHYDAQM
                     SDSFFANLEIPRPDVNLKVQSSGAVTQIADIMTRLEPVVEGIRPDVVLVVGDVNSTVA
                     AALTAVKLGRPVAHVEAGLRSFDRGMPEEINRILTDSISDLLFTTEPAGNENLAREGI
                     PASKVHFVGNVMIDTLFRYRELAGQSDVLGRLGLAAGQYVALTLHRPSNVDDEGTLAV
                     LLAAVARIQAEIPIVFPVHPRTRRRLEALAAVLPPMPALRLADPLPYLDFLQLTANAR
                     CVLTDSGGIQEETTALGVPCLTLRQNTERPITVTKGTNRVIGTDEETIYRSWRSVADG
                     QWPRGELPELWDGKAAGRIVQVLLGMGR"
     gene            complement(3079..>3559)
                     /locus_tag="VNU03_06535"
     CDS             complement(3079..>3559)
                     /locus_tag="VNU03_06535"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HWP74150.1"
                     /translation="TLWSLKVLAEAGFQYDSSIFPIVHDRYGIPDAPRFPHRLAFGPS
                     LELVEFPLSTVRLLRGRVPVAGGGYFRLFPYWLTRRAIRHLNHREGKPAIVYLHPWEI
                     DPGQPRLPVGRFAHLRHTLNMRSTESKLRRLLRDFRFGPVRDVLAASGALASAGGVA"
BASE COUNT          576 a         1221 c         1238 g          524 t
ORIGIN      
        1 ggtcaccacc accgggatcc caccgcccat gggttggagc acggtctggc gcatgttgtc
       61 gctcgtccag agcggcgcga ccgccaccga ggcccgggcc agtgccggcc gcatgtccgg
      121 gcgtggcgcg tcgacctcga caccggggat ccggtacgtg agcgccgcgt cgcgtggcga
      181 ctcgtggctg ctgatcacca ggcggacttc cgggtattgc gagcggacgg tgggcatcac
      241 gtggcggatg aactccagcg catcgtggct ctccgacgca ccgcccaggg cagtggccag
      301 gactagggtc ggcggggctc cccgccgcgg ccgcgcccgg aagttctcca cgtcgacccc
      361 gtccgccacg agggtgaccg ggccggccgg cttgaaaccc cgcaccagct tcgcggcctc
      421 cggcgtcgcc accagacagt ggcccgcgcg ccgcgccgcc agagcttcgg tggcgcggag
      481 ccggacgccc tcggtccgga agaaccggga tccggggaac gatccgcgcg aggcctgccg
      541 cgtccaccac tcggagtcga cgtgggcgaa gtcgacgatc accgggatcg aggggtcgac
      601 ctccatcgca tactggatca tgctggacga cgtcacgaac accagatcgt agggcgtggt
      661 ccggatccgg tcggcgagtc gcgatcggag cgcctccgag cggaaatacg agagcgtgca
      721 cgactcgccg gtcatcaggc tgaccgcgct ggcgagcgac ttccacgtcc gcggcaccac
      781 cgtgaactcg agatccccga actccgaccg gagcgacgag atcggcccga ccgcgttggg
      841 gttgtccgtc gtgaacgcga gagtgaggct gtgcttctgg gctagcaccc ggccgaagtg
      901 atactcgcgc gtcgccttga ggggatagcg cggcgccccg acccaccttc cgagcatcag
      961 gatgttcatg gcaggtaccg ggtcagccac ggccccacga ccttggtcac gggcaggggc
     1021 aggcgcttcc acaggtccac cgcggtccgc atcttgggat tcgacgggct caggttcggc
     1081 atcgggtgtc catcgaggag gatgtactga tacggcagcg gcaccggctc gaagccccag
     1141 tgacgtttga agtgatacgc cccggtgccc tcgcggctac gtccgaagtc gaagctccgg
     1201 ctcccgatcc tcgcggcgtg gcaggtcagc tcccagtaca tgaagtcgtt caccgcgtac
     1261 ggcagggcct cgtgcagtgc gcccccgtag taggggagca cctgatcctc gaagaacagg
     1321 ctgagcaccc cggccaccgt cgtctcttcc ttccagatcg tgaggagctc gcactccttg
     1381 ccgaactcct gggcgatcgc ctggaagagc cgccgcggaa acaccggtga gcccagggcg
     1441 tgtacgctgt gggcgtagac gtcccagaac ccgtcgagat ggcgcaggcc gagctccgcc
     1501 cgcagcccgt gcttcaggcc ctggcgcgtc atccggcgct gctttcgcgg aatcgcggcg
     1561 aggttctcgt cctcgctggc cgagatgggc cgggagaagg tcacgtagag cgtcttggtc
     1621 ggcagatcga gggcccgacc ggggcgatgg cgaagctcga cgtaagacgc cttcacgcgg
     1681 cgggccagct cggtcgcggc ctgcacgagg gcggacgccg cgctcgcgga ggcggcgcag
     1741 acgcctccgt agacgccgta ggggaccgag acgagccccc gcccgccgaa cagtccgcgc
     1801 acctcgaaca gggggaggac tcccgccacc gcgcccccct cgaacgccag aaggtagtgc
     1861 ggccggtgcc ggaaggccgt ctcgaccgca cgcttccagg cgatcagatg gaacgggctg
     1921 ccgcccggcg tgcttcgcac gaaggcgtcc cattccacgg tctcggtggt cagcggtcgg
     1981 acgtccacgg tcgtcagcgc cccattccga ggagtacctg cacgatccgg cccgcggcct
     2041 tgccgtccca cagctccggc agctccccac ggggccactg gccgtccgcg accgagcgcc
     2101 agctccgata gatcgtctcc tcgtcggtac cgatcacccg gttggtcccc ttggtcaccg
     2161 tgatcgggcg ctcggtgttc tgccgcagcg tgaggcacgg gactccgagc gcggtcgtct
     2221 cctcctggat gcctcccgag tcggtcaaga cgcaccgggc gttcgcggtc agctggagga
     2281 agtccagata cgggagggga tccgccagcc gcagcgccgg catcggcggc agcaccgcgg
     2341 ccagcgcctc gagtcgccgc cgggtacgtg ggtgcacggg gaagacgatc gggatctcgg
     2401 cctggatgcg tgcgaccgcg gccaggagga ccgccagcgt tccctcgtcg tccacgttgc
     2461 tcggccggtg cagggtcaag gcgacgtact gcccggccgc gagaccgagg cggccgagga
     2521 cgtccgactg gccggccagc tcccggtagc ggaacagggt gtcgatcatc acgttgccca
     2581 cgaagtgcac cttgctcgcc gggatgccct cgcgggccag gttctcgttg cccgccggct
     2641 ccgtggtgaa gaggaggtcc gagatcgagt cggtcaggat gcgattgatc tcctccggca
     2701 tgccccgatc gaagctacgc agtcccgcct ccacgtgcgc gaccgggcgc ccgagcttca
     2761 cggcggtgag agccgcggcc accgtcgagt tcacgtcacc caccaccagc accacgtcgg
     2821 gacggatacc ctccacgacc ggctccaggc gggtcatgat gtcggcgatc tgggtcaccg
     2881 cgcccgagga ctgcaccttc aggttgacgt ccggacgcgg aatctcgaga ttcgcgaaga
     2941 aggagtcgga catctgcgcg tcgtagtgtt gcccggtgtg cacgagcgtg gtggtgagat
     3001 acgggtactc ccgcagctcg gccatgatcg gcgcgatctt gacgaagttc ggacgtgccc
     3061 cgacgacggc caggatcttc acgccacgcc tcccgcgctg gccagggccc ccgacgcggc
     3121 caggacgtcc cgcacgggtc cgaagcgaaa gtcccgaagc agccggcgta gcttggactc
     3181 ggtcgagcgc atgttgaggg tatgacggag atgggcgaac cggcccaccg gaagccgcgg
     3241 ctgtcccggg tcgatctccc atggatgcag gtacacgatc gctggcttgc cctcgcggtg
     3301 attcagatga cggatggccc ggcgggtgag ccagtacgga aacagccgga agtatccccc
     3361 tcctgctacc ggaacacggc cgcgcaacag ccgcaccgtc gaaagcggaa actcgaccag
     3421 ctcgaggctc ggtccgaacg cgagccggtg gggaaaacgt ggcgcgtcgg ggatcccgta
     3481 gcggtcgtgc acgatgggga agatgctcga gtcgtattgg aagccggcct cggcgaggac
     3541 cttgaggctc cacagggtc
//
LOCUS       DATMCV010000282         2140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_213760, whole genome shotgun sequence.
ACCESSION   DATMCV010000282 DATMCV010000000
VERSION     DATMCV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213760"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VNU03_06540"
     CDS             <1..648
                     /locus_tag="VNU03_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWP74151.1"
                     /translation="VDYQVAVSMLQGISTLGVVPLARVPWNDPARLMKILDAGAYGVI
                     CPMINTAQEAEAMVRACKYPPRGFRSWGPVRASLYAGSDYGDHANVETIVMPMIETAE
                     ALKNLDEILSVDGVDAIYVGPSDLSLAMGLKPRLDQTDPPVVDAQKKIAAACKRHNVI
                     AGIHNATAAYSLKMIAEGYQFVTLASDSRHMAAKAAEEVGIVRKTGTKTGKLPAY"
     gene            648..1271
                     /locus_tag="VNU03_06545"
     CDS             648..1271
                     /locus_tag="VNU03_06545"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWP74152.1"
                     /translation="MPARLDGALLDRARANLAAFERLALPDDGRRRAAVALVLLPDGD
                     GRPCFLITRRAATLRQHTGQWALPGGRVDAGETAERAALRELQEEVGLTLHESTVLGV
                     LDDYGTRSGYIITPVVVWAEPDHELVPNPAEVAKIYRVPLDDLEGPDVPRLISIPESD
                     RPVIQLPLLGTLIHAPTAAVLYQMREVVVHGRPTRVNHFEQPVWAWR"
     gene            1303..2091
                     /locus_tag="VNU03_06550"
     CDS             1303..2091
                     /locus_tag="VNU03_06550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008110127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP74153.1"
                     /translation="MGALDGQVAIMTGGGSGIGRAAALMLAAEGAQVVVAGRRKAPVE
                     EVAGEIARAGGRAVARTCDVEKPAEARALAEWTVAQYGRADILVNNAGHSSKARNVRW
                     VGQEEWDSVLAVNLTGVYALTQAVLPSMIERGGGTIITISSLAALKPGLIGGAPYGAA
                     KAAVLNLMGHVHNVLRDKGIRATTIMPAEVDTPILDKRPFRPDAKARSTMMLAEDVAR
                     AILLCATLPPRTVIETIVMSPTILRDQSQDIKVARDEGATPGAR"
BASE COUNT          334 a          755 c          792 g          259 t
ORIGIN      
        1 gtcgactacc aggtcgcggt gagcatgctc cagggcatct cgaccctggg cgtggtgccg
       61 ctggcccggg tgccgtggaa cgaccccgcg aggctcatga agatcctcga cgcgggcgcc
      121 tacggcgtca tctgcccgat gatcaacacc gcgcaggagg cggaggcgat ggtgcgcgcg
      181 tgcaagtacc cgccgcgcgg cttccgcagc tggggcccgg tgcgcgcctc gctctacgcc
      241 ggctccgatt acggcgacca cgccaacgtc gagaccatcg tgatgccgat gatcgagacc
      301 gcggaggcgc tcaagaacct cgacgagatc ctgagcgtgg acggcgtgga cgccatctac
      361 gtgggcccct ccgacctgag cctggccatg ggcctcaagc cgcggctcga tcagaccgat
      421 ccgccggtgg tggacgccca gaagaagatc gcggccgcct gcaagcggca caacgtgatc
      481 gcgggcatcc acaacgccac cgcggcctat tccctcaaga tgatcgcgga gggctaccag
      541 ttcgtgaccc tggcgagcga cagccgacac atggcggcca aggccgccga ggaagtcggc
      601 atcgtgcgca agaccggcac caagaccggg aagctgccgg cctactagtg ccggcccggc
      661 tggacggcgc gctgctcgat cgggcgcgcg ccaacctggc cgccttcgag cggctcgcgc
      721 tgcccgacga cgggcggcgc cgggccgcgg tggccctggt gctgctgccc gacggggacg
      781 gtcgcccgtg cttcctcatc acccggcggg ccgcgacgct gcgccagcac accggccagt
      841 gggccctgcc gggcgggcgc gtcgacgcag gcgagaccgc ggagcgcgcg gcgctgcgcg
      901 agctgcagga ggaggtcggc ctgaccctcc acgagagcac cgtgctgggc gtgctggacg
      961 actacggcac ccgctcgggc tacatcatca ccccggtggt ggtatgggcc gagcccgatc
     1021 acgagctggt gccgaacccg gccgaggtcg cgaagatcta ccgggtgccg ctcgacgatc
     1081 tggagggccc ggacgtgccg cggctcatca gcatcccgga gagcgaccgg ccggtgatcc
     1141 agctcccgct gctcggcacg ttgatccacg cgccgaccgc ggcggtgctc taccagatgc
     1201 gcgaggtggt cgtccacgga cgacccacgc gcgtgaacca cttcgagcag cccgtgtggg
     1261 cctggcgctg aggcgccggg caccccgggc ggaaggagcc tcatgggagc tctcgacggg
     1321 caggtcgcga tcatgacggg cggcggcagc ggcatcggcc gcgccgcggc gctgatgctg
     1381 gccgccgaag gggcgcaggt ggtggtggcg gggcggcgaa aggccccggt cgaggaggtg
     1441 gccggcgaga tcgcgcgggc gggcggccgc gcggtggcca ggacctgcga cgtggaaaag
     1501 cccgccgagg cccgcgccct cgcggagtgg acggtggccc agtacgggcg cgccgacatc
     1561 ctggtgaaca acgctgggca ctcgagcaag gcccgcaacg tccggtgggt cggtcaggag
     1621 gagtgggaca gcgtgctcgc ggtgaacctg accggggtct acgcgctcac ccaggcggtg
     1681 ctcccgagca tgatcgagcg cggcggcggc accatcatca cgatcagctc gctggccgcg
     1741 ctgaagccgg gcctgatcgg cggcgccccc tacggggcgg ccaaggccgc ggtgctcaac
     1801 ctgatggggc acgtccacaa cgtgctccgc gacaagggga tccgggccac caccatcatg
     1861 cccgccgagg tcgacacccc gatcctcgac aagcggccct tccgacccga cgccaaggcc
     1921 cgctccacca tgatgctcgc ggaggacgtg gcccgggcga tcctgctctg cgcgacgctg
     1981 cctccgcgca ccgtgatcga gaccatcgtg atgagcccga cgatcctgcg ggaccagagc
     2041 caggacatca aggtggcgcg cgacgagggg gcgacgccgg gcgcgaggta gacggaccgc
     2101 tcagcccgcc gcggggggcg gcccgatcga ggcctgctgc
//
LOCUS       DATMCV010000283         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_416829, whole genome shotgun sequence.
ACCESSION   DATMCV010000283 DATMCV010000000
VERSION     DATMCV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416829"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VNU03_06555"
     CDS             complement(<1..462)
                     /locus_tag="VNU03_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP74154.1"
                     /translation="MTERIGDLVLEHAAPAGRAHSRSVLFVHGMWGGSWYLRNYLYAA
                     AQSGWDAWAVNLRGHGESPAEGGLGSVSFLDYVDDVRRCLDRLGEAVLVGHSMGGLIA
                     QKVVEGGGVSAAVFMTSAPPRGIVTLEWPVLVRATRYLPAVLSGRAFTMTRA"
     gene            complement(566..1711)
                     /locus_tag="VNU03_06560"
     CDS             complement(566..1711)
                     /locus_tag="VNU03_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP74155.1"
                     /translation="MINAPRLKEEFIELVSISSPSKREGTIARRLEAILKSMGGSVEV
                     DGAGEKIEGNTGNLLARFPGNTPGAPSFLLSGHMDTVGPAEKIRPVVEGDVVRTDGTS
                     VLGGDDKAGVVAILEAIRVLREKSIPHGDIEVVLTICEEYGLLGAKHFDTGRLRARRG
                     LVLDVDGVCELITRAPAANRMAFTVHGLPAHAGICPEQGMSAIQIAAEAIAAMKLGRV
                     DAETTANLGLIQGGLATNIVPAMLTVRGEARSLSVEKLEAQTLHMRRCFDEAVRRHSV
                     RVGDRSYQARVEAQVDRQYSRLDIRNDASIVRLVQAAATATGRTCPTRSTGGGSDANV
                     FAERGLEVANLACGMREIHTVNEWVDVRDLVLTAGLVVETVRLNAAA"
     gene            complement(1708..>2474)
                     /locus_tag="VNU03_06565"
     CDS             complement(1708..>2474)
                     /locus_tag="VNU03_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP74156.1"
                     /translation="RQYLPPLRPRLGVSESRFRVTVGAAEVAGVLHLPEDGRGRRPCV
                     VACHGMGASKDSDKYLLLGRELPSAGLALARFDFRGSGESGGRHEDATIETRMADLEG
                     VLDHLAKRPELDGRFGLLGSSLGGFVALWVAAARAGGAPLPVVTWNAPASLRELPGAD
                     PDQAGRALVAEVRRGERADAPAGVARVLVIQADRDEVVPPAHGRRLFERAAEPRALHV
                     IAGADHRLTDPGHRREALEESRRFLLHHLAPQRTAP"
BASE COUNT          333 a          886 c          924 g          331 t
ORIGIN      
        1 cgcgcgggtc atcgtgaacg cgcggccgct gagcaccgcg ggcaggtagc gggtcgcgcg
       61 cacgagcacc ggccactcga gggtcacgat cccgcgcggg ggcgcgctgg tcatgaacac
      121 cgcggccgag accccgccgc cctcgaccac cttctgggcg atgagcccac ccatcgagtg
      181 gccgaccagc accgcctccc cgagccgatc caggcagcgg cggacgtcgt cgacgtagtc
      241 gaggaaggag accgagccga gcccgccctc ggccgggctc tccccgtgac cgcgcaggtt
      301 gacggcccag gcgtcccagc cggactgggc ggcggcgtag agatagttgc gcaggtacca
      361 gctgccgccc cacatgccgt ggacgaagag gacagaccgg gaatgggccc gtccggcggg
      421 ggcggcatgc tcgaggacca ggtcgccgat gcgctcggtc aaatcgtatc gtctcggggt
      481 cgtatcgtcc cgggtgggat ccgccccggt atcttacgcc aaccggccgt cccgcgggcg
      541 gtgggggtca cgggagacgg acgggtcagg ccgcggcgtt gaggcggacg gtctcgacca
      601 ccaggccggc ggtgagcacc aggtcgcgca cgtcgaccca ctcgttgacg gtgtggatct
      661 cgcgcatgcc gcaggcgagg ttggcgacct cgaggccgcg ctccgcgaag acgttggcgt
      721 ccgagccgcc gccggtggag cgcgtgggac aggtgcgacc ggtggcggtg gcggcggcct
      781 gcaccaggcg gacgatcgag gcgtcgttcc ggatgtcgag ccgcgagtac tgccggtcga
      841 cctgggcctc gacgcgggcc tggtagctcc ggtcgcccac ccggaccgag tgccgccgga
      901 ccgcctcgtc gaagcagcgc cgcatgtgaa gcgtctgggc ctcgagcttc tccacgctca
      961 ggctgcgggc ctcaccgcgc accgtcagca tcgcgggcac gatgttggtg gcgagcccgc
     1021 cctggatcag gccgagattc gcggtggtct ccgcgtcgac cctcccgagc ttcatcgccg
     1081 cgatggcctc cgccgcgatc tggatcgcgc tcatcccctg ctccgggcag atgccggcgt
     1141 gggcgggcag gccgtgcacg gtgaaggcca tgcggttggc cgcgggggcg cgggtgatca
     1201 gctcgcagac cccgtccacg tcgaggacga ggccgcgccg cgcccggagc cggccggtgt
     1261 cgaagtgctt ggcgccgagc aggccgtatt cctcgcagat ggtcagcacc acctcgatgt
     1321 ctccgtgcgg gatcgacttc tcgcggagca cccggatcgc ctccaggatc gccaccaccc
     1381 ccgccttgtc gtcgcccccc agcacgctgg tgccgtcggt gcgcaccacg tcgccctcca
     1441 ccaccgggcg gatcttctcg gcgggaccca ccgtgtccat gtggccggag agcaggaacg
     1501 acggcgcgcc cggggtgttg ccggggaatc gcgcgaggag gttgccggta ttgccctcga
     1561 tcttctcgcc cgcgccgtcg acctccacgc tgccgcccat ggacttgagg atcgcctcga
     1621 ggcgccgcgc gatcgtgccc tcgcgcttcg acgggctcga gatcgagacg agctcgatga
     1681 actcttcctt cagccgtgga gcgttgatca tggagcggtc ctctgggggg cgagatggtg
     1741 gagcaggaag cgccggctct cctcgagcgc ttcccggcga tggcccggat cggtgaggcg
     1801 gtggtcggcc cccgcgatga cgtgcagggc tcggggttcg gcggcgcgct cgaagagccg
     1861 ccggccgtgg gcgggcggca ccacctcgtc gcggtcggcc tggatcacga gcacccgggc
     1921 gacgccggcg ggcgcgtcgg cccgctctcc ccgccgcacc tccgcgacga gggcgcggcc
     1981 ggcctggtcc ggatcggcgc cgggcagctc gcgcagtgac gcgggcgcat tccaggtcac
     2041 gaccggcagc ggggcgccgc ccgcccgcgc ggcggccacc cagagcgcga cgaagccgcc
     2101 gaggctcgag ccgagcagcc cgaagcggcc gtccagctcg ggccgcttgg ccaggtggtc
     2161 gagcacgccc tccagatcgg ccatgcgcgt ctcgatggtg gcgtcctcgt ggcggccgcc
     2221 cgattcgccg ctgccgcgga agtcgaagcg cgcgagggcc aggcccgcgg agggaagctc
     2281 gcgtccgagg aggaggtact tgtcgctgtc cttggaggcg cccatgccgt ggcaggccac
     2341 tacgcagggc cggcggccac gcccgtcctc gggcagatgg agcacccccg cgacctcggc
     2401 ggcgccgacg gtgacgcgga agcgcgactc gctcacgccc agacgaggtc gaagaggcgg
     2461 caggtattga cggt
//
LOCUS       DATMCV010000284         2503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_949663, whole genome shotgun sequence.
ACCESSION   DATMCV010000284 DATMCV010000000
VERSION     DATMCV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949663"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VNU03_06570"
     CDS             <1..469
                     /locus_tag="VNU03_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74157.1"
                     /translation="AARREKLTRERIAAASSALTGGGKGPVLAQMAETAGDSAGALAE
                     VYRAQTEYVAGAMAEGADGAEARRAHEATTVLQTNMERTRANLERANEAGLAMTMRLS
                     QSGVLEKVAQLETIARDAGARLAARWELERAARERDREQREREAGERARQQRR"
     gene            492..1205
                     /locus_tag="VNU03_06575"
     CDS             492..1205
                     /locus_tag="VNU03_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP74158.1"
                     /translation="MPRIPDLSDPRYLDEVGWFLYHEKHRRDRFGGSYNAERLAYSRL
                     LRDEVAAFLGRDTRWFEGKTVVSIGCGCTGDLAAFPATVKVAIDPLLYAYQHLDMLVP
                     DEAGGRTLYLSLGAEELPLLDRAADLVICRNALDHMPDPRVALNEMWRILSDDGALFV
                     SVDLGGDPTPDEPTVFSVESLGALLKERFEVVTLSGQHRPHSASRTGNVRALARKIPD
                     AGRSLDREQILMAYEARLP"
     gene            1210..2457
                     /locus_tag="VNU03_06580"
     CDS             1210..2457
                     /locus_tag="VNU03_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP74159.1"
                     /translation="MPVGPTLVILGMMGRMPVAGVTWQVLHYLEGFRRLGFDAYYVED
                     TGEWSYDPDQNTISEDPTYAVNSIRAVMALHRFPDRWAYRAADGRIFGLPAPELGRLF
                     ERAEVLVNLTGATVLRDEHRRVPVRLYLETDPVLPQIEVATGNRFHVDLLSAHTHHFT
                     YGENIGQPDCGVPVSRFPYRPTRPPVVLDWWPSAAVSPGPCFTTVASWRQAGKDVEWR
                     GHTYTWSKHHEFLKIIDLPRRTPQPLELALAVRGRAADGETAWVPRDQEDAEAIQSLT
                     RHGWRVVNAMPLSHDNARYRDYILASRGEFTVAKDQNVRLRSGWFSDRSACYLAAGRP
                     VVTQDTAFGKFVPTGEGLFAFASLDDILAALEAINSDYGRHARAAREVAETYFRAETV
                     LARLVEDAGVSPSLRNPGMMRQP"
BASE COUNT          385 a          906 c          872 g          340 t
ORIGIN      
        1 ggccgcgcgc cgcgagaagc tcacccggga gcggatcgcc gccgcgagct ccgccctgac
       61 cggcggcggc aagggccccg tgctggccca gatggccgag accgccgggg acagcgccgg
      121 cgcgctggcc gaggtgtacc gggcgcagac cgagtacgtc gcgggcgcga tggcggaggg
      181 ggcggacggg gcggaggcca ggcgggccca cgaagccacc accgtgctgc agacgaacat
      241 ggagcggacc agagcgaacc tggagcgcgc aaacgaggcc ggcctggcga tgaccatgcg
      301 gctgtcgcag tcgggagtgc tcgagaaggt ggcgcagctc gagaccatcg cccgggacgc
      361 gggcgcgcgg ctcgccgcgc gctgggagct cgagcgggcc gcacgcgagc gcgaccggga
      421 gcaacgagag cgagaagccg gcgagcgcgc caggcagcag cggcggtgac cggtccgagt
      481 ggaggagagc gatgccacgg atccccgacc tttccgaccc ccgctacctg gacgaagtgg
      541 ggtggttcct gtaccacgag aagcaccggc gtgaccgctt cggaggctcc tacaacgccg
      601 agcggctcgc gtactcccga ctcctgcggg acgaagtcgc cgcctttctg ggccgggaca
      661 cccgctggtt cgagggcaag accgtcgtca gcatcgggtg cggctgtacc ggagacctcg
      721 ccgccttccc ggcgaccgtc aaggtcgcga tcgaccccct gctctatgcg taccagcacc
      781 tggacatgct ggttcccgac gaggcgggcg gccgcacgct ctacctgtcg ctgggagccg
      841 aagagctccc cctgctggat cgcgccgccg acctcgtgat ttgccggaac gccctcgatc
      901 acatgcccga tccccgcgtg gcgctgaacg agatgtggcg gatcctgtcc gacgacggcg
      961 ccctgttcgt gagcgtggat ctcgggggcg acccgactcc cgacgagccg accgtcttct
     1021 cggtggagag tctgggcgcc ctcctgaagg agcgcttcga ggtcgtcacc ctctccggcc
     1081 agcaccgtcc gcacagtgcc agccggaccg ggaacgtccg ggccctcgcg cggaagatcc
     1141 ccgatgccgg ccgctccctc gaccgggagc aaatcctgat ggcgtacgaa gcgcgtctcc
     1201 cgtagaccga tgcccgtcgg gccgacgctg gtcatcctgg gcatgatggg tcgcatgccg
     1261 gtcgccggcg tgacctggca ggtccttcac tacctcgagg gcttccgccg gctcggcttc
     1321 gacgcgtact acgtggaaga caccggcgag tggtcctacg accccgacca gaacacgatc
     1381 agcgaggacc ccacctacgc ggtcaactcc atccgcgcgg tcatggccct ccaccgcttc
     1441 ccggatcggt gggcctatcg cgccgcggac gggcggatct tcgggctccc cgcccccgag
     1501 ctcggccggc tcttcgagcg ggccgaggtg ctcgtcaacc tcaccggggc aaccgtgctg
     1561 cgcgacgagc accggcgcgt gccggtgcgc ctctacctcg agacggatcc ggtgctgccg
     1621 cagatcgagg tcgcgaccgg gaaccgcttc cacgtcgatc tgctgagcgc ccacacccac
     1681 cacttcacct acggcgagaa catcggacag cccgattgcg gggtcccggt ctcgcgtttt
     1741 ccctatcgcc cgacccgtcc gccggtggtg ctcgactggt ggccgtccgc cgccgtctcc
     1801 cccggtccgt gcttcaccac cgtggcgagc tggcgccagg ccggcaagga cgtcgagtgg
     1861 cgaggtcaca cgtacacgtg gagcaagcac cacgagttcc tcaagatcat cgacctgccc
     1921 cgccggacgc cgcagcccct ggagctggcc ctcgcggtgc ggggccgcgc ggcagacggg
     1981 gagaccgcgt gggtgccgcg ggaccaggag gacgccgagg ccatccagag cctgacgcgg
     2041 cacggatggc gcgtcgtcaa cgcgatgccg ctgtcccacg acaacgcgcg ctatcgcgac
     2101 tacatcctcg cctcgcgcgg ggagttcacg gtggccaagg accagaacgt ccgcctgcgg
     2161 agcggctggt tcagcgatcg gagcgcctgc tatctcgccg cgggccggcc ggtggtcacc
     2221 caggacaccg ccttcgggaa gttcgtcccg accggcgagg ggctgttcgc gttcgcgtcc
     2281 ctcgacgaca tcctcgccgc gctcgaggcc atcaactccg actacgggcg ccacgctcga
     2341 gcggcccgcg aggtcgccga gacctacttc agggccgaga cggtgctggc gaggttggtc
     2401 gaggatgccg gagtgagccc ctcgctgcga aatccaggta tgatgcgcca gccatgaccc
     2461 gcgtcctgac cggcgccacc ctgatcgacg ggaccggcgc agc
//
LOCUS       DATMCV010000285         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_880555, whole genome shotgun sequence.
ACCESSION   DATMCV010000285 DATMCV010000000
VERSION     DATMCV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880555"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1091)
                     /locus_tag="VNU03_06585"
     CDS             complement(90..1091)
                     /locus_tag="VNU03_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74160.1"
                     /translation="MNASRSPEMPRRTFMTVVAGSLLVAPRAARAQPVAKVWRIGLFH
                     VGLDHVPPSLDGLRDGLKALGYEEGRNLQLDFRNVADEAAARVTAADLARGRPDLIVA
                     FENQTLRAAKAATSDIPVVFLHVTDPVGDGVALSLAHPGRNMTGFVALGNAPSKEAEL
                     FKQLVPGLRHLLALTDPDDPGGRAYRRQVRQVGAALKFQLVERDVRSQADIERVFASL
                     SHGAVDGVFIASLNLRVKFHSLILRLASERRLPVAGHRKEWVEQGALFSYAENIREVG
                     RAAAARYVDKILKGARPADLPIDAYEHPELAINLKTARTLGLTIPPALLQRADQVIE"
     gene            complement(1162..1482)
                     /locus_tag="VNU03_06590"
     CDS             complement(1162..1482)
                     /locus_tag="VNU03_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74161.1"
                     /translation="MPTQEPTREEVVTKTLDTLDRPDITHPADINNLMTVLLGFPQME
                     QNKPCPNANQIWRRAVPYLLAYRYSDCESLKREEFPEDTLGAWMKQALRDELRRRSVK
                     SDEA"
     gene            complement(1868..>3254)
                     /locus_tag="VNU03_06595"
     CDS             complement(1868..>3254)
                     /locus_tag="VNU03_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74162.1"
                     /translation="ENLDPTMNLSSIRAKVGVSLFDSLVGRDADNKLVPQLAESWRAL
                     DDVTWQLKLRRGVVFHNGEPFNAEAVRFTFERVLDPNQKSPNRANVGEVARVEVVDDY
                     TVNLVLRQPYAPLLNRLIDFPIVPPKYTAEKGNQGLALRPVGTGPFRFVELVKDDHLI
                     VEAFDRHWRGAPKVRRIVYKPIPEPFTRAAALRNNEVDLIDTVPPNLASELERAGGIR
                     VQRVPSTWIIYLGLNALKKPLSDVRVRQALNYATDADALVKSVLDGNARRMDGPFTPH
                     MFGFDPSVKGYAPDPARARRLLAEAGYPDGLEITLEAPAGRYQGDKEIAEALGGQWQK
                     AGFKPRVQVAEWGAYFKRYLGKQFQDAYLLGLGGPMQDGDELYNLVSSKGRGLYYKND
                     KVDALFDAGRTTMDPAKRKKVYADLARAMVEDATWVFLMQQVDIYASRERVTWTPRSD
                     QWMPLHEAIVK"
BASE COUNT          476 a         1084 c         1128 g          566 t
ORIGIN      
        1 gcccgatcac ggccggccgg cttccgcagg aagacccgct tgcccacggc gggccggagc
       61 ggcgctcgag gcatgcgagg acctccgggc tactcgatca cctggtccgc ccgttgaagg
      121 agcgcgggcg ggatcgtcag gccgagggtc ctggcggtct tgaggttgat ggcgagctcg
      181 gggtgctcgt aggcatcgat cggaaggtcg gcgggcctgg cgcctttcag gatcttgtcc
      241 acgtagcgcg ccgccgccgc gcgcccgacc tcgcggatgt tctcggcgta cgagaagagc
      301 gcgccctgct ccacccactc cttgcggtga cccgccaccg ggaggcgccg ctcggaggcg
      361 aggcgaagga tgagcgagtg gaacttcacg cgcagattca gcgaggcgat gaacacgccg
      421 tcgaccgcac cgtgggagag cgaggcgaat acgcgctcga tgtcggcctg cgaccggacg
      481 tcacgctcca ccagctggaa cttgagcgcc gcccccacct ggcggacctg gcgacgatag
      541 gcccgcccgc ccggatcgtc cgggtccgtc agggcgagga gatggcggag accgggcacg
      601 agctgcttga acagctccgc ctccttcgac ggcgcgttcc cgagggcgac gaagccggtc
      661 atgttgcggc ccggatgcgc gaggctcagg gcaaccccgt cccctaccgg gtcggtcacg
      721 tggaggaata ccaccgggat gtcgctggtc gccgccttcg cggcgcggag cgtctggttc
      781 tcgaaggcga cgattaggtc cgggcgccct cgagccaggt cggcggcggt gacgcgcgcc
      841 gcggcctcgt cggcgacgtt ccggaagtcg agctggaggt tcctgccttc ctcgtagccc
      901 agcgccttca ggccgtcgcg gaggccatcg agggagggag gcacgtggtc gaggccgaca
      961 tggaacagcc cgatccgcca taccttcgcg accggctgcg cgcgggccgc gcgcggtgcg
     1021 acgaggaggc tgccggcgac gaccgtcatg aaggtccgtc gcggcatctc cggcgagcga
     1081 ctggcgttca tgtggtcctc cgcccgatgg ctagcgacac tgtatcaccc gcgccgctcg
     1141 cctcccgccc ttgaatcagc atcaagcttc gtccgacttc actgaccggc gtcggagttc
     1201 gtctcgcagg gcttgcttca tccacgcgcc caaggtgtct tcagggaact cctctcgctt
     1261 cagagattcg caatcggagt acctatacgc gagcagatag ggtaccgccc gtcgccatat
     1321 ctgattcgcg tttgggcagg gcttgttctg ttccatctgg ggaaagccca acagaacggt
     1381 catgaggttg ttgatgtcgg ccgggtgcgt gatgtcgggg cggtccagag tgtcgagtgt
     1441 tttggtgaca acctcctcgc gggtgggctc ctgggtgggc atctccgtta cctctccact
     1501 ctctggtctg ggacctcatc gcggtacacg tcggctagca ccgccttcgg acgacatctc
     1561 ttccctcgga gcttgctcag gacgtggcag cgcgcttgca gggcgggctc accgtggtgg
     1621 gcgcaatgtc ctgcggggcc tgggcgctcg gatcgttgtg catggccccg aggtggagtc
     1681 cacgggcaca atcggacaaa tgatttcccg gaagatgcgt ttgccaaacc cccagctttc
     1741 gtgggcggcc cgtggataag tcggtgggct cgcgccgctc cggctcggga tttcacgcac
     1801 taagagcaag tcgccttgtt tctgtgcagc caccgctgaa ccgcgattcg ccgattcgcg
     1861 gggccggcta cttgacgatc gcctcgtgca gcggcatcca ctggtcgctg cgcggagtcc
     1921 acgtcacgcg ctcgcggctc gcgtagatgt cgacctgctg catcaggaac acccacgtgg
     1981 cgtcctcgac catcgcgcgg gccagatccg cgtagacctt cttccgcttc gccgggtcca
     2041 tcgtggtgcg gcccgcgtcg aagagcgcgt ccaccttgtc gttcttgtag tagaggccgc
     2101 ggcccttcga cgagaccagg ttgtacagct cgtcgccgtc ctgcatgggg ccgcccaggc
     2161 cgaggaggta ggcgtcctgg aactgcttgc cgagatagcg cttgaagtag gcgccccact
     2221 cggccacctg cacgcgcggc ttgaagcccg ccttctgcca ctggccgccg agcgcttccg
     2281 cgatctcctt gtcgccctga tagcgccccg ccggcgcctc gagcgtgatc tcgaggccgt
     2341 ccggataccc ggcttccgcc agcaggcggc gggcccgggc cggatccggc gcgtagccct
     2401 tcaccgacgg atcgaagccg aacatgtgcg gggtgaacgg gccgtccatc cggcgggcgt
     2461 tgccgtcgag cacgctcttg acgagcgcgt ccgcgtcggt cgcgtagttg agggcctggc
     2521 gcacccgcac atcggagagc ggcttcttga gggcgttgag ccccagatag atgatccacg
     2581 tgctgggcac gcgctggacc cggatgccgc ccgcccgctc cagctcggac gccaggttgg
     2641 gcggcaccgt gtcgatcagg tcgacctcgt tgttgcgcag ggcggccgcg cgggtgaagg
     2701 gctccgggat cggcttgtag acgatgcgac ggaccttcgg ggcgccccgc cagtgccggt
     2761 cgaaggcctc cacgatcagg tggtcgtcct tcaccagctc gacgaagcgg aacgggccgg
     2821 tgcccaccgg gcgcagggcc aagccctggt tgcccttctc cgcggtgtac ttcggcggga
     2881 cgatcgggaa gtcgatgagc cggttgagga gcggtgcgta gggctggcgc agcaccagat
     2941 tgaccgtgta gtcgtccacg acctcgacgc gcgccacctc gcccacgttc gcccggttgg
     3001 gcgacttctg gttggggtcg agcacccgct cgaaggtgaa gcgtaccgcc tccgcgttga
     3061 acggctcgcc attgtggaac acgacgccgc ggcgcagctt gagctgccac gtcacgtcgt
     3121 cgagcgccct ccaggactct gcgagctggg gaacgagctt gttgtcggcg tcgcggccca
     3181 ccagcgagtc gaagaggctc acgcccacct tcgcgcggat ggacgagagg ttcatggtcg
     3241 ggtcgaggtt ctcc
//
LOCUS       DATMCV010000286         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_214020, whole genome shotgun sequence.
ACCESSION   DATMCV010000286 DATMCV010000000
VERSION     DATMCV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214020"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VNU03_06600"
     CDS             <1..248
                     /locus_tag="VNU03_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74163.1"
                     /translation="ASLTDVHWTMSGEVVLMTLLGGLGTVFGPVVGAVIVIGLETYLA
                     QLGAWVTVVQGTIFVLCVLAFRRGVVGELARLLRKPL"
     gene            complement(232..540)
                     /locus_tag="VNU03_06605"
     CDS             complement(232..540)
                     /locus_tag="VNU03_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74164.1"
                     /translation="MLGPEQDCERPAEARAFIARLRLAEIEGAEDIHREAMWQAFGRC
                     PSGAAGGACRAEEQRRFDARWEVERREIEAKYRDVLGAFEQRCRAVISLIGWAVRAAS
                     "
     gene            808..>2012
                     /locus_tag="VNU03_06610"
     CDS             808..>2012
                     /locus_tag="VNU03_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP74165.1"
                     /translation="MLDPSPDTFPKLALQNARRLSGKVAIREKHLGIWQSYTWSQYAE
                     QARLIALGLAALGFARGDKTAVVGDNRPALYWAMLATQALGGVAVPLYQDSIEKEMEY
                     IVDHAEARFAIVEDQEQVDKLLHVREQCPGLEYVIYDDPGGLRHYKDSCLLSLQELQE
                     RGRKFAEDRPGYFQQEVAKGAAEDTAVIAYTSGTTGQPKGAMLSHRNLIETARSAVSR
                     ERLDETEEVMAYLPMAWIGDHMFSFSQSMVAGFTTNCPESAETVLADLKEIGPTYFFA
                     PPRIWENILTSVMIRVDDTAWIKRGLVRFFLDLARRVERCRLGHRPVALRDRLLYPLG
                     RLLVYGPLRDNLGMSRIRVAYTAGEAIGPELFEFYRSLGINVKQLYGMTESSALICIQ
                     QDGDVKLDTV"
BASE COUNT          309 a          708 c          674 g          321 t
ORIGIN      
        1 tcgcgtcgct caccgacgtg cactggacga tgtcgggcga ggtggtgctg atgacgctcc
       61 tcggcgggct cgggaccgtg ttcgggcccg tggtgggcgc ggtgatcgtc atcgggctcg
      121 agacctacct ggcccagctc ggggcctggg tcaccgtggt gcagggaacc atcttcgtcc
      181 tgtgcgtgct ggccttccgg cgcggggtcg tgggcgagct cgcccgtctt ctcaggaagc
      241 cgctctaact gcccatccga tcaggctgat gacggctcga cagcgctgct cgaatgcgcc
      301 gagcacgtca cggtacttgg cttcgatttc ccggcgctcg acctcccacc gcgcgtcgaa
      361 ccgccgctgc tcctctgccc gacaggctcc tccggcggcc cctgatgggc agcggccgaa
      421 tgcctgccac atggcctccc ggtggatgtc ctccgcccct tcgatctcgg ccaggcgcag
      481 gcgggcgatg aaggcacggg cctcggccgg gcgctcgcag tcctgctccg ggccgagcac
      541 ggccgggaca ccgggcgcga ggggcccggt cgcccagagg actgtcagcc agatgaccgt
      601 aaatatcgcc gtaaaccctt gtcctggcgg ccgaagtctt gacaccccgg aggccctggg
      661 ctagactctc gccactctat cacccccggc aacgggcgct tcccggcgag cgcttcctta
      721 ggcaatgacg gaaattctct ccaatggtgg aggtggctgt cgtctcgcgc gaccccgcga
      781 gaatggcgcc cacatccgcg cggcctgatg ctcgatccgt ccccggacac ctttcccaag
      841 ctcgcgctcc agaacgcccg gcggctctcc ggcaaggtcg ccatccgtga gaagcacctc
      901 gggatctggc agtcgtacac ctggagccag tacgcggagc aggcccgcct gatcgccctg
      961 ggcctggccg cgctcggctt cgcgcgcggg gacaagaccg cggtggtcgg cgacaaccgg
     1021 cccgcgctgt actgggcgat gctggccacg caggcgctcg ggggggtcgc ggtcccgctc
     1081 taccaggact ccatcgagaa ggagatggag tacatcgtcg accacgcgga ggcccgcttc
     1141 gcgatcgtcg aggaccagga gcaggtcgac aagctgctcc acgtccggga gcagtgcccc
     1201 gggctcgaat acgtcatcta cgacgacccg ggagggctcc ggcactacaa ggattcctgc
     1261 ctgctgagcc tccaggagct gcaggagcgc gggaggaagt tcgccgagga ccggccgggc
     1321 tacttccagc aggaggtggc caagggcgcg gccgaggata cggcggtcat cgcctacacc
     1381 tccgggacca ccggtcagcc caagggggcc atgctctccc accgcaacct catcgagacc
     1441 gcgcgcagcg cggtctcgcg ggagcggctc gacgagaccg aggaggtcat ggcctacctg
     1501 ccgatggcgt ggatcggcga ccacatgttc tccttcagcc agtcgatggt ggcgggcttt
     1561 accaccaact gccccgagag cgccgagacg gtgctggccg acctcaagga gatcggcccg
     1621 acctacttct tcgcgccgcc gcgcatctgg gagaacatcc tcacctcggt gatgatccgg
     1681 gtggacgata ccgcctggat caagcgcggc ctggtgcgct tcttcctcga cctggcgcgc
     1741 cgcgtggagc gctgccgtct cggccaccga ccggtcgcgc tgcgggaccg gctgctctac
     1801 ccgctcgggc gactgctggt ctacggcccg ctgcgggaca acctcggcat gagccgtatc
     1861 cgggtcgcct acaccgcggg ggaggccatc ggacccgagc tgttcgagtt ctaccggtcc
     1921 ctcggcatca acgtgaagca gctctacggc atgaccgagt cgagcgcgct gatctgcatc
     1981 cagcaggacg gcgacgtcaa gctcgacacg gt
//
LOCUS       DATMCV010000287         3169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_615973, whole genome shotgun sequence.
ACCESSION   DATMCV010000287 DATMCV010000000
VERSION     DATMCV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615973"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VNU03_06615"
     CDS             <1..141
                     /locus_tag="VNU03_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWP74166.1"
                     /translation="EMVMPGDNVTMTIELIQPVAMEKELRFAIREGGRTVGAGVVAEI
                     TD"
     gene            145..465
                     /gene="rpsJ"
                     /locus_tag="VNU03_06620"
     CDS             145..465
                     /gene="rpsJ"
                     /locus_tag="VNU03_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWP74167.1"
                     /translation="MVTVSADQKIRIRLKAYDHNLLDRSMKEIVETVRRTGARVTGPV
                     LLPTMINRWTVLRSPHVDKTSREQFEMRTHKRLIDILDPTPQTVDALMKLDLPSGVDV
                     EIKL"
     gene            497..1177
                     /gene="rplC"
                     /locus_tag="VNU03_06625"
     CDS             497..1177
                     /gene="rplC"
                     /locus_tag="VNU03_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWP74168.1"
                     /translation="MAMKEGLIGRKKGMTQVFGEDGNLIPVTVVEAGPCTVVGLRTKP
                     THGYDAIQLGFEPRKKNVNKAMAGLYKKAGVAPAMRVLREIRLQKTEAVSAYQVGQTL
                     TVDLFTPGEYVDVVGTTKGKGFQGGVRRYGWAGGDETHGSMFHRRPGSIGASSDPSRV
                     WPGHHLPGRMGADRRTVLNVVVARVIPEQNLVLLRGAVPGAIGGLVVIRKSVKQTKAQ
                     QQKAKEAK"
     gene            1180..1809
                     /gene="rplD"
                     /locus_tag="VNU03_06630"
     CDS             1180..1809
                     /gene="rplD"
                     /locus_tag="VNU03_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWP74169.1"
                     /translation="MPSVPIVDAKGKSQQTLELSADVFGVTPRVPLLHQAVVKELAAR
                     RAGTHSTRGRSDVRGGGRKPWKQKGTGRARQGSIRATQWKGGGKPFGPRPRKYDKAMP
                     STMRRAALREAVAAKLGAGHVSVVDTLGVAEAKTKALTTRLKGLGIDGQPTLLVVAQV
                     TDALTRAARNVPWLSVELPGHTSVYQVIRNDRLVFEKAALLTLQETLKP"
     gene            1806..2096
                     /gene="rplW"
                     /locus_tag="VNU03_06635"
     CDS             1806..2096
                     /gene="rplW"
                     /locus_tag="VNU03_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWP74170.1"
                     /translation="MRDPRSVLVRPLMTEKSMQQKEAHNMVTFEVAVDANKVEIRHAV
                     EKVFNVKVADVRTQAREGKWKRMGKFEGRRRGWKKAMVTLAPGHKIELVEGA"
     gene            2101..2928
                     /gene="rplB"
                     /locus_tag="VNU03_06640"
     CDS             2101..2928
                     /gene="rplB"
                     /locus_tag="VNU03_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006445778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWP74171.1"
                     /translation="MGIRTVKPTSPARRYMSFLTNEEITKKTPEKSLLRSKGNRTNGR
                     NVYGRITVRHRGGGVKRMLRDVDFRREKIGIPAKVAGIEYDPNRSARIALLHYRDGEK
                     RYIIAPLGLKPGDVIMSGPQADILPGNALPIRNIPLGTLVHNVELQPGKGGQLCRSAG
                     TLAQLLAKEGDHANIKLPSGEVRQVKIDCMATIGQVGNLDHENVSIGKAGRVRWKGFR
                     PTVRGTVMNPVDHPMGGGEGKGKGNHPMTPWGKPTKGYKTRRGARPSDRYIVTRRTK"
     gene            2947..>3169
                     /gene="rpsS"
                     /locus_tag="VNU03_06645"
     CDS             2947..>3169
                     /gene="rpsS"
                     /locus_tag="VNU03_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWP74172.1"
                     /translation="MGRSLKKGPYIDERLEKRVDELNRTRQKKVLKTWARRSTISPEF
                     VGHTLAVHNGKKFIPVYIAENMVGHRLGEF"
BASE COUNT          595 a         1006 c         1184 g          384 t
ORIGIN      
        1 gagatggtga tgccggggga caacgtgacg atgacgatcg agttgatcca gccggtggcg
       61 atggagaagg agctgcggtt cgcgattcgg gaggggggcc ggacggtggg cgccggcgtg
      121 gtcgcggaga tcacggacta gcccatggtt accgtgagcg ccgaccagaa gatccgcatc
      181 cggctcaagg cgtacgacca caatctcctc gaccggtcca tgaaggagat cgtggagacg
      241 gtgcgccgga ccggcgcgcg cgtcacgggc cccgtgctgc tgcccaccat gatcaaccgg
      301 tggacggtgc tgcgctcccc gcacgtggac aagacctcgc gcgagcagtt cgagatgcgc
      361 acgcacaagc ggctcatcga catcctggac ccgaccccgc agacggtgga cgcgctgatg
      421 aagctcgatc tgccgagcgg cgtcgacgtc gaaatcaagc tctagtccgg gcccgagaga
      481 tagcgagaac ctcctcatgg cgatgaagga agggctgatc ggccgcaaga agggcatgac
      541 ccaggtcttc ggggaggacg gcaacctcat cccggtgacg gtggtcgagg cgggcccctg
      601 cacggtggtg gggctgcgga ccaagcccac ccacggctac gacgccatcc agctcggctt
      661 cgagccgagg aagaagaacg tcaacaaggc gatggcgggg ctctacaaga aggcgggggt
      721 cgcccccgcg atgcgggtgc tgcgggagat ccggctccag aagacggagg cggtgagcgc
      781 gtaccaggtg ggccagaccc tcacggtgga cctcttcacg cccggcgagt acgtggacgt
      841 ggtggggacg accaagggca agggcttcca gggcggcgtg cggcgctacg gctgggcggg
      901 cggcgacgag acccacggct ccatgttcca ccggcggccg gggtccatcg gcgcctcctc
      961 ggatccgtcc cgggtctggc cgggccatca cctgccgggc cgcatgggcg cggaccggcg
     1021 caccgtcctc aacgtggtgg tggcgcgggt gatcccggag cagaacctgg tcctgctccg
     1081 cggcgcggtg ccgggagcca tcggagggct ggtggtgatc cgcaagagcg tgaagcagac
     1141 caaggcgcag cagcagaagg cgaaagaggc caagtagcga tgcccagcgt gccgatcgtc
     1201 gacgcaaagg ggaagtccca gcagacgctc gagctgtccg ccgacgtgtt cggcgtcact
     1261 ccgcgggtgc cgctcctgca ccaggcggtg gtgaaggagc tcgccgcgcg ccgggcgggc
     1321 acgcactcca cccgtgggcg cagcgacgtg cggggcggcg gccgcaagcc gtggaagcag
     1381 aagggcaccg gccgcgcccg gcagggctcg atccgggcca cccagtggaa gggcggcggc
     1441 aagccgttcg gcccccggcc gcgcaagtac gacaaggcga tgccgtcgac gatgcggcgc
     1501 gccgcgctgc gcgaggcggt cgcggccaag ctcggagccg gccacgtgtc ggtggtcgac
     1561 accctcgggg tcgcggaggc caagaccaag gccctcacca cgcggctgaa ggggctcggc
     1621 atcgacgggc agcccacgct cctggtggtg gcccaggtga ccgacgcgct cacccgcgcc
     1681 gcccgcaacg tgccgtggct gtcggtggag ctgcccgggc acacctcggt ctaccaggtg
     1741 atccgcaacg accggctcgt gttcgagaag gcggcgctgc tgacgctgca ggagacgctc
     1801 aagccatgag ggacccccgc tcggtgctgg tgcggccgct gatgaccgag aagagcatgc
     1861 agcagaagga agcccacaac atggtcacgt tcgaggtggc ggtggacgcc aacaaggtgg
     1921 agatccgcca cgcggtggag aaggtcttca acgtgaaggt ggcggacgtg cgcacccagg
     1981 cccgcgaggg caagtggaag cgcatgggca agttcgaggg acggcgccgg ggctggaaga
     2041 aggcgatggt caccctcgcc cccggtcaca agatcgagct cgtggagggg gcctaggacc
     2101 atgggaatcc gcacggtcaa gccgacctcg ccggcccgac gctacatgtc gttcctgacg
     2161 aacgaggaga tcaccaagaa gaccccggag aagagcctcc tccggtccaa gggcaaccgg
     2221 acgaacgggc gcaacgtcta cggccgcatc acggtgcggc accggggcgg cggggtcaag
     2281 cgcatgctcc gggacgtgga cttccggcgc gagaagatcg ggatcccggc gaaggtggcg
     2341 gggatcgagt acgacccgaa ccgctccgcc cgcatcgcgc tgctccacta ccgcgacggg
     2401 gagaagcggt acatcatcgc gccgctgggg ctcaagccgg gcgacgtgat catgtcgggg
     2461 ccgcaggccg acatcctgcc cggcaacgcg ctgccgatcc ggaacatccc gctcggtacc
     2521 ctcgtgcaca acgtggagct gcagcccggc aagggcggcc agctctgccg gagcgcggga
     2581 acgctggcgc agctgctcgc caaggaaggc gatcacgcca acatcaagct gccctcgggc
     2641 gaggtgcggc aggtcaagat cgactgcatg gccaccatcg gccaggtggg caacctggat
     2701 cacgagaacg tctcgatcgg caaggcgggg cgcgtgcggt ggaagggctt ccgacccacg
     2761 gtgcgcggca ccgtgatgaa cccggtggac caccccatgg gcggcggcga gggcaagggc
     2821 aagggcaacc acccgatgac cccgtggggc aagcccacca agggctacaa gacgcggcgc
     2881 ggggcgaggc cgtccgatcg ctacatcgtg acccggcgga cgaaataggg agcgagggca
     2941 gaagccatgg gacggtcgct gaagaagggc ccgtacatcg acgagcgtct ggagaagcgc
     3001 gtggacgagc tgaaccgcac gcgccagaag aaggtgctga agacgtgggc gcggcgctcg
     3061 acgatctcgc cggagttcgt gggccacacc ctcgcggtgc acaacgggaa gaagttcatc
     3121 ccggtgtaca tcgccgagaa catggtgggg caccggctgg gcgagttcg
//
LOCUS       DATMCV010000288         6468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_677879, whole genome shotgun sequence.
ACCESSION   DATMCV010000288 DATMCV010000000
VERSION     DATMCV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677879"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..1235
                     /locus_tag="VNU03_06650"
     CDS             495..1235
                     /locus_tag="VNU03_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWP74173.1"
                     /translation="MAPGEESSTLSVVRPAAVPSRPSAEAFPPLYRLVRGACAPVLRR
                     LFDLRVEGAEHLPASGSFILAANHHNYLDGVVLGVAVPRPIAFLVMPRVYRATPLHPS
                     LHRRIGSIPVNLERPDPGAIKRVLRALDAGRIVGIFPEGPFSQEGRLVTGQPGAALIA
                     LRAGVPIVPAAIEGTYEALRSRRFYVPRRRPLSVRFGAPLVFDRPRHRPIARAEREEV
                     TRRIMSEIAGLFHPASEPAAAAGRAGAS"
     gene            1232..2641
                     /gene="argH"
                     /locus_tag="VNU03_06655"
     CDS             1232..2641
                     /gene="argH"
                     /locus_tag="VNU03_06655"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWP74174.1"
                     /translation="MTAAGTPPGKAWSGRFTEGADPTAEAFTSSLSFDRRLWPYDLVG
                     SAAWARALERAKLITPAELEDLLAGIEAVRAELAGGRFPFRRELEDIHMNVERRLVEL
                     AGSVGGKLHTGRSRNDQIALDERLYLRDVIDGVDAGLREVQRALVAQAEKHRDAPMPG
                     YTHLQRAQPVLLAHHLLAYVFMLERDRQRFRDCRARVNVMPLGAAALAGTAFAIDREA
                     LARDLGMSGPSANSMDAVADRDFLVEFLAHAAIVGMHGSRLAADLTLWATAEFGFVEF
                     SDAFATGSSIMPQKKNPDVAELVRGKTGRLYGNLTAALTTMKGLPLTYNSDMQEDKEP
                     LFDTVDTLEAIFRVLPPMLGSLTFRVDRMRDAAGAHYSTATDLADYLVRQGLPFRDAH
                     EVVGKAVRHAITRGRELGDLPIEELRQFSSLIGRDVYAALTVEASLRARGVTGGTAPE
                     SVRQQLAQVRSWLAQEPGA"
     gene            2638..3720
                     /locus_tag="VNU03_06660"
     CDS             2638..3720
                     /locus_tag="VNU03_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74175.1"
                     /translation="MSPATRRRLLALCLAVGALAACGKKGPPVAPEIRVPTVPASLHG
                     AVDEQSIVVSWNAPGTRVDGSRLRTIALYRLYRREEADSGPAKSAMLSSGHIVGYDEV
                     ATIRPEAPAPATVQGGSVTWVDRRALSMGRRYVYVVTAEDELGRTSGPSGRLIVPFLT
                     APPAPRGLTGAPGDRRVILTWQAPAITEAAGGSTGEIRYLVLRGVGPTGALASITAEP
                     VAGTTFTDGGVDNDVDYRYAVRSVRVETAVTATGEASTPIMVAPANTTPPGAPTGLVA
                     VPTSGAVRLAWNPSPETDVATYAVYRAGESGDFMRIATAVPPGTVYIDRDVRAGSTYR
                     YAVTAIDRARKPNESARSNVVSVRVE"
     gene            complement(3724..4983)
                     /locus_tag="VNU03_06665"
     CDS             complement(3724..4983)
                     /locus_tag="VNU03_06665"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWP74176.1"
                     /translation="MDSHPTPVRRAQLHADVAAEVRRRFGTPCYVYDRAALESAARAA
                     LAFPAPFGLTLRYAMKANPSRGILTLFRDLGLHVDASSDHEVERALRAGFSPERIQLT
                     SQMPSRTLKEHVGRGVLFNACSLHQLEEFGRIAPGGEVSVRVNPGLGTGSTKRTNTGG
                     PASSFGIWHEAMGEVAAIAKRHRLRIRRLHSHVGSGTDPEVWKRCARMTLDLVGALPD
                     VATVNLGGGFKVGRMPEEPSVDLADVGAHVRREIEAFRDRDGRALHLEIEPGTFLVAN
                     AGVIVASCVDVVDTGADGYAFAKLDAGMPEITRPSLYGAQHPIDVLARGREQAAIVFV
                     GPCCESGDILTPAPGDPEALAPRWVPRPQIGDLVVVGGAGAYCAGMATINYNSYPQAP
                     EVMLDADGSLTLLRRRQVPEQVWQNEV"
     gene            5104..5997
                     /gene="dapA"
                     /locus_tag="VNU03_06670"
     CDS             5104..5997
                     /gene="dapA"
                     /locus_tag="VNU03_06670"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWP74177.1"
                     /translation="MRPTFQGSIVALVTPFRSGKVDEAKLRELVEWHVASGTDGIVPC
                     GTTGESPTLSHDEHKRVVEIVIETARGRLAVIAGTGSNSTAEAIELTAHARKAGATGA
                     LVVNPYYNKPTQEGLYRHFRAIADAVDIPILVYNIAGRTAVNVETDTLARLARDCGNI
                     VGVKEASGSLDQMTQVILACGPEFSVLSGDDNVTLPLMSVGGRGVISVIANLVPRETA
                     EMTHAALAGDWKQARELHLKLFPLSRAMFLQTNPIPVKEALAMMGKLAEAEFRLPMCR
                     LSDAHRPRLKAVLASLGLVKG"
     gene            6008..>6468
                     /gene="dapB"
                     /locus_tag="VNU03_06675"
     CDS             6008..>6468
                     /gene="dapB"
                     /locus_tag="VNU03_06675"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWP74178.1"
                     /translation="MADVVVAGAAGRMGSRLVALLQQETELRLVAALEAPGHPALGRD
                     AGESAGVGRLDIPIGADVDAVLGKDRILIEFSVPEASLAHARLVGRHGGRAVIGTTGF
                     TAAQREELAGIGRATPLLVSPNMSVAVNVAFKVLADMARMLGDDYDVEVVET"
BASE COUNT          862 a         2365 c         2324 g          917 t
ORIGIN      
        1 ggcgctaacc gcccgctgag cgatcctcaa ctttgatttg tggatcactc gttgcgggca
       61 atctcggcct ttgcgggggc ccgcaaccac cgatacaaac atcctcagct tcgagatcga
      121 tttgacctac cgtgttgcgc gaagaggatt gatagaggcg cgattctgtc ctggcatcca
      181 cggcctcatt tcaggcgtga cggctgagac gagcggcgct gcccgccttg ccctcgccct
      241 cgacgccttc atccccgagc atcgcgactc ctggcggttg tttgggtagg ggctggagag
      301 cgtggaggat gctgaagccc tgctagtcga gtgcgatgga tgcgagaccg cgagggtgag
      361 cggtgggaag gctcgagaca cggaccgccc agagccgagg cccgcgattc ctccctatgg
      421 ggtggcttgg cgtggcttcg gggggggtgg tggcgcggtt gtcaggtggg ggggtggatg
      481 ctataagggg cgctatggcg ccgggagagg agtcctccac cctgtccgtg gtgcggccgg
      541 ccgccgtgcc gtcccgcccg agcgccgagg cgtttccgcc gctgtatcgc ctggtgcgcg
      601 gcgcgtgcgc gccggtgctg cgccggctct tcgacctgcg cgtggagggg gccgagcacc
      661 tgccggccag cggctcgttc atcctcgccg cgaaccatca caactatctc gatggcgtgg
      721 tgctgggcgt cgcggtgccg cggccgatcg cgttcctcgt catgccgcgg gtctatcgcg
      781 ccaccccgct gcacccgtcg ctccaccggc gcatcggctc gatcccggtg aacctggagc
      841 ggccggaccc gggggcgatc aagcgcgtgc tgcgcgcgct cgacgcggga cgcatcgtcg
      901 ggatctttcc cgagggcccg ttcagccagg agggccggct cgtgaccggc cagcccggcg
      961 cggccctcat cgcgctgcgc gccggcgtgc cgatcgtgcc cgccgcgatc gagggcacct
     1021 acgaggccct gcggtcgcgg cggttctacg tgccccgccg ccggcccctc tcggtgcgct
     1081 tcggcgcgcc gctcgtgttc gatcgcccgc gccatcggcc catcgcgcgc gccgagcgcg
     1141 aggaggtcac ccgccgcatc atgtcggaga tcgccgggct gttccatccg gcctccgagc
     1201 ccgccgcggc cgccggccgc gcgggtgcct cgtgaccgcc gccggcacgc ccccgggcaa
     1261 ggcctggtcc gggcggttca ccgagggcgc cgatccgacc gcggaggcct tcacctcctc
     1321 gctgtccttc gaccggcggc tctggccgta cgacctggtg gggagcgcgg cgtgggcgcg
     1381 cgcgctcgag cgggcgaagc tgatcacgcc cgccgagctc gaggatctgc tcgccgggat
     1441 cgaggcggtg cgggcggagc tcgcgggggg ccggttcccg ttccgccgcg agctcgagga
     1501 catccacatg aacgtggagc ggcgcctggt ggagctggcc ggatcggtgg gcggcaagct
     1561 ccacaccggg cggtcgcgca acgaccagat cgcgctcgac gagcgcctct acctgcgcga
     1621 cgtcatcgac ggggtcgacg cggggctccg cgaggtccag cgggcgctgg tggcccaggc
     1681 cgagaagcat cgcgacgcgc cgatgcccgg ctacacccac ctgcagcggg cgcagccggt
     1741 gctgctggcc caccatctgc tcgcctacgt gttcatgctc gagcgcgacc ggcagcggtt
     1801 ccgcgactgc cgcgcgcggg tcaacgtgat gccgctcggc gccgccgcgc tcgccggcac
     1861 cgccttcgcg atcgaccgcg aggccctcgc gcgcgacctc ggcatgagcg gcccgagcgc
     1921 caacagcatg gacgcggtcg ccgaccgcga cttcctggtc gagttccttg cccacgccgc
     1981 catcgtgggc atgcacggct cgcggctggc cgccgatctc accctgtggg ccaccgccga
     2041 gttcggcttc gtggagttct cggacgcctt cgccaccggc tcgtcgatca tgccgcagaa
     2101 gaagaacccc gacgtggccg agctggtccg cggcaagacc gggcgcctct acggcaacct
     2161 gaccgcggcc ctcaccacca tgaaggggct gccgctcacc tacaactccg acatgcagga
     2221 ggacaaggag ccgctcttcg acaccgtcga cacgctcgag gcgatcttcc gcgtgctgcc
     2281 cccgatgctc ggctcgctga ccttccgggt ggaccggatg cgcgacgcgg cgggcgcgca
     2341 ctactcgacc gccaccgacc tggccgacta cctggtccgc cagggcctgc ccttccggga
     2401 cgcgcacgag gtggtgggca aggcggtgcg ccacgcgatc acgcgcggcc gcgagctggg
     2461 cgacctgccg atcgaggagc tgcggcagtt ctcgtcgctg atcggacgcg acgtctacgc
     2521 ggccctgacc gtcgaggcct cgctgcgcgc gcgcggggtg accgggggca ccgcccccga
     2581 gtcggtgcgg cagcagctcg cgcaggtccg cagctggctc gcgcaagagc cgggcgcgtg
     2641 agcccggcca cgcggcggcg cctgctcgcg ctgtgcctgg ccgtcggcgc cctcgccgcc
     2701 tgcggcaaga aaggcccgcc ggtggcgccc gagatccggg tgcccaccgt cccggcctcc
     2761 ctgcacggcg cggtggacga gcagtccatc gtggtgagct ggaacgcccc cggcacgcgg
     2821 gtcgacggca gccggctgcg cacgatcgcg ctctaccgcc tctatcgccg ggaggaagcc
     2881 gactcggggc cggccaagtc ggccatgctc tcgtcggggc acatcgtggg ctacgacgag
     2941 gtcgcgacca tccgaccgga ggcgcccgcg cccgcgaccg tgcagggtgg ctcggtgacg
     3001 tgggtggatc ggcgcgcgct ctcgatgggg cggcgctacg tgtacgtggt caccgccgag
     3061 gacgagctcg ggcggacgag cggcccctcc ggccgcctga tcgtgccgtt cctgaccgcg
     3121 cccccggcgc cgcgcgggct caccggcgcg ccgggggatc gccgcgtcat cctcacgtgg
     3181 caggcgccgg cgatcacgga ggccgcgggg ggctccaccg gcgagatccg gtacctggtc
     3241 ctgcgcgggg tcgggccgac cggcgccctg gcttcgatca cggccgagcc ggtcgccggc
     3301 acgaccttca cggacggagg cgtcgacaac gacgtggact accgctacgc ggtgcgctcg
     3361 gtccgggtgg agaccgcggt caccgcgacg ggggaggcgt cgacgccgat catggtcgcg
     3421 cccgcgaata ccacgccgcc cggcgcgccc accggcctgg tcgcggtgcc gacgagcggc
     3481 gcggtgcgcc tggcctggaa cccgagtccg gagacggacg tggcgaccta cgcggtgtat
     3541 cgggcgggcg agagcgggga cttcatgcgc atcgccaccg cggtgcctcc cggcaccgtc
     3601 tatatcgacc gggacgtgcg ggcgggctcg acctatcgct acgcggtcac cgccatcgat
     3661 cgcgcccgca agcccaacga gagcgcgcgg tccaacgtgg tctcggtgcg cgtcgagtaa
     3721 cccttagacc tcgttctgcc agacctgctc cggcacctgg cggcgccgca gcagcgtgag
     3781 gctcccgtcg gcgtcgagca tgacctcggg agcctgcggg taggagttgt agttgatggt
     3841 cgccatgccc gcgcagtacg cgcccgctcc gcccaccacc accaggtcgc cgatctgcgg
     3901 gcgcggcacc cagcgcggcg cgagcgcctc cggatcgccg ggggccggcg tgaggatgtc
     3961 gcccgactcg cagcacggcc ccacgaagac gatcgcggcc tgctcgcgcc cgcgcgcgag
     4021 cacgtcgatg gggtgctggg ccccgtagag cgacggccgc gtgatctcgg gcatgcccgc
     4081 gtcgagcttg gcgaaggcgt agccgtcggc cccggtgtcg accacgtcca cgcacgaggc
     4141 cacgatgacc cccgcgttgg cgaccaggaa ggtgccgggc tcgatctcga ggtgcagcgc
     4201 gcgaccatcg cggtcgcgga aggcctcgat ctcgcgccgc acgtgcgccc ccacgtcggc
     4261 caggtccacc gagggctcct ccggcatccg ccccaccttg aagccgcccc ccaggttgac
     4321 ggtcgccacg tcgggcagcg cccccaccag gtcgagcgtc atgcgcgcgc agcgcttcca
     4381 cacctcggga tcggtgccgg agccgacgtg cgagtgcagg cggcggatgc gcaggcggtg
     4441 gcgcttggcg atggcggcca cttcccccat cgcctcgtgc cagatcccga agctcgaggc
     4501 ggggccgccg gtgttggtgc gcttggtgga gccggtgccg agccccgggt tcacccggac
     4561 cgacacctcg ccgccgggcg cgatccgccc gaactcctcg agctgatgca gcgagcaggc
     4621 gttgaagagc accccgcgcc ccacgtgctc cttgagggtc cgcgacggca tctgcgaggt
     4681 gagctggata cgctcgggcg agaagccggc gcgcagggct cgctccacct cgtgatcgct
     4741 ggaggcgtcc acgtgcaggc cgaggtcgcg gaagagggtg aggatgcccc ggctcgggtt
     4801 ggccttcatc gcgtagcgca gggtcaggcc gaagggcgcg gggaacgcga gcgcggcccg
     4861 ggccgccgac tccagcgcgg cgcgatcgta gacgtagcac ggcgtgccga agcggcggcg
     4921 cacctcggcc gcgacgtcgg cgtggagctg ggcccgtcgc acgggcgtgg ggtgactgtc
     4981 catccgcagg agtctaccgc atcgcggcag ccgcttgaag tgcttcctcc tgtgatgtta
     5041 gagtcaagga actcttcgtc ccacatcggc cccgattggg atgagcaacg gggggactca
     5101 accatgcgcc cgactttcca aggctcgatc gtcgctctcg tgacgccgtt ccgctccggc
     5161 aaggtggacg aggccaagct gcgcgagctg gtcgagtggc acgtcgccag cggcaccgac
     5221 ggtatcgtgc cctgcggcac caccggcgag tcccccaccc tcagtcacga cgagcacaag
     5281 cgcgtggtgg agatcgtgat cgagaccgcg cgcggccgcc tcgcggtcat cgcgggcacc
     5341 ggctcgaact ccaccgccga ggccatcgag ctcaccgccc acgccaggaa ggcgggcgcc
     5401 accggcgcgc tggtggtgaa tccctactac aacaagccca cccaggaggg cctctatcgc
     5461 cacttccgcg cgatcgcgga cgcggtggac attccgatcc tggtctacaa catcgcgggg
     5521 cgcaccgcgg tgaacgtgga gaccgacacc ctggcccgcc tcgcccgcga ctgcggcaac
     5581 atcgtgggcg tcaaggaagc ctcgggctcg ctcgaccaga tgacccaggt catcctcgcc
     5641 tgcggccccg agttctcggt cctctccggc gacgacaacg tcacgctgcc gctcatgtcg
     5701 gtgggcgggc gcggggtgat ctcggtcatc gccaacctcg tgccgcgcga gaccgccgag
     5761 atgacccacg ccgccctcgc gggtgactgg aagcaggcgc gcgagctgca cctcaagctg
     5821 ttcccgctct cgcgggcgat gttcctccag accaatccga tcccggtcaa ggaggccctg
     5881 gccatgatgg gcaagctggc ggaggccgag ttccggcttc ccatgtgccg cctgtccgac
     5941 gcccaccggc ctcgcctcaa ggcggtcctg gcctcgctcg gcctcgtcaa gggctgaccc
     6001 gccctccatg gcggatgtcg tcgtcgccgg cgccgccggc cgcatgggaa gccgtctcgt
     6061 cgcgctgctc cagcaggaga ccgagctgcg cctggtcgcc gcgctcgagg ccccgggaca
     6121 tcccgcgctc gggcgcgacg cgggcgagtc ggccggcgtc gggcggctcg acatcccgat
     6181 cggggccgac gtggacgcgg tcctcggaaa ggaccgcatc ctcatcgagt tctcggtgcc
     6241 ggaggcgagc ctcgcccacg cccgcctggt gggccgtcac ggcggccgcg cggtcatcgg
     6301 caccaccggc ttcaccgcgg cccagcgcga ggagctggcc ggtatcggcc gcgccacgcc
     6361 cctgctcgtg tcgcccaaca tgagcgtggc ggtcaacgtc gcgttcaagg tgctcgccga
     6421 catggcccgg atgctgggcg acgactacga cgtggaggtc gtcgagac
//
LOCUS       DATMCV010000289         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_417127, whole genome shotgun sequence.
ACCESSION   DATMCV010000289 DATMCV010000000
VERSION     DATMCV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417127"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..>2932)
                     /locus_tag="VNU03_06680"
     CDS             complement(27..>2932)
                     /locus_tag="VNU03_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP74179.1"
                     /translation="LYFPSPFSWRVPTLEEGAVTIGLGLLASVIPAGLYGRMRNHYAW
                     RVVLSVSFTAYSYLFVFISGGSIEMHFHFFMIMALLVMYSDWRLGWLVLVLTALHHAV
                     LNYLQPNWVYFYGRNDFAIVAHGLPVGVMALFTTRLCQIQRQSVAILEETRRVQEEDL
                     LKRREAEETLRESEEKSRYVIESAADAIVSIDAEGAVREFNRAAERLFGYAAAEMAGE
                     PLTRLIPERFHEAHRADLHRSLKSGERRFRSWRNIDLVGLTQDGREVPLETSFSRFEA
                     RGRSFFTGVLRDITQRKQVEAELAAARDRALEASRLKSEFLARMSHEIRTPMNGIIGM
                     TGLLLDTELTARQRHHAETVRKSADALLTIINDILDFSKIEAGRLRLEPVSFDIMIAV
                     EEVGELLSGAAAEKGLDLIIRVAPDVPRYVVGDPGRIRQILINLTNNAIKFTATGHVL
                     LNLECEESGGGEVRLRFGVSDTGIGIADDRIEHIFDKFTQVDASSTRRYGGTGLGLAI
                     SRELVNLMGGTLGVTSRLGEGSTFTARVRLQMGAASPRATAAAPQLSGVRALIVDDNE
                     INRQVLRERLASWRMRADDVASAAAAMAALRAAAGAGDPYGIVLTDFEMPDGDGETLA
                     RSVQSDPVLRGVPMVLLTSVDQPDGADFVRAAGFGAYLVKPVRASLLMDALLTVWGAR
                     LESETGEMVTRATLMARRGEPAQVAGAGTLLHVRVLLVEDNPVNQQVATAMLEQLGAR
                     VDVAADGKEALEQVALLPYDLIFMDCEMPEMDGYAATAEIRRREDGGRRIPIVAMTAH
                     AMEGDRERCLAAGMDDYITKPVSPAAIGAVGRRWVRASGPDKAAGPAPRPPAALDATR
                     IAQLRATLGRGNGAQFRKLVETFLGDAGVRLGELRRCLNRKDEAMVRRLAHTLRGSCL
                     NLGAVGMTEVASALERASAQGDGAAASLVDQLEAEFRRAESALVALLEPGQTS"
BASE COUNT          473 a         1073 c          943 g          443 t
ORIGIN      
        1 ggcatcgtcg tcgaccatga ggatcttcat gacgtctgcc ccggctcgag gagggcgacg
       61 agcgcggact cggcccgtcg gaactccgcc tcgagctggt cgaccagtga ggccgccgcc
      121 ccgtcccctt gcgccgacgc gcgctccaga gcgctcgcca cttcggtcat gcccaccgcg
      181 ccgagattca gacagctacc tcgcagcgtg tgcgcgagcc gacgcaccat ggcctcatcc
      241 tttcgattca ggcaccgccg gagctctccg aggcggacac ccgcgtcgcc gaggaaggtc
      301 tcgacgagct tgcgaaactg ggctccattc ccccgtccaa gggtggcccg aagctgggcg
      361 atccgagtgg catccagcgc cgccggcggt ctgggtgcgg ggccggccgc cttgtcgggc
      421 ccggaagccc gcacccaccg gcggccgaca gccccgatcg ccgcggggct gaccggcttg
      481 gttatgtaat cgtccatccc ggcggccaga caccgctcgc ggtccccctc catggcgtga
      541 gcggtcatcg ccacgatcgg gatccggcgg cccccgtctt cccggcgcct gatctcggct
      601 gtcgctgcgt acccgtccat ctcgggcatc tcgcagtcca tgaagatgag gtcatacggc
      661 agaagcgcta cctgctcgag cgcctccttc ccgtcggcgg cgacgtccac gcgcgcccca
      721 agctgctcca gcatcgcggt cgcgacctgc tggttgaccg gattgtcctc gaccagcagc
      781 acccggacgt ggagcagcgt accggctcca gcaacctgtg ccggctcgcc ccggcgcgcc
      841 atcagcgtcg cccgcgtcac catctccccg gtctcacttt ccagccgagc gccccacacc
      901 gtcagcagcg catccatgag gagcgacgcc cgaaccggtt tgaccagata ggcgccgaag
      961 ccggcggccc gaacgaaatc ggcaccgtcg ggctgatcca ccgacgtcag cagcaccatg
     1021 ggcaccccgc gcaacacggg atcgctctgc accgaccgcg cgagcgtctc cccgtccccg
     1081 tccggcatct cgaagtccgt gagcacgatg ccgtagggat cgccggcccc cgccgcggcc
     1141 cgcagcgcgg ccatggccgc cgcggcagac gccacgtcgt cggctcgcat ccgccagctc
     1201 gcgagccgtt cgcgtagcac ctgtcggttg atctcgttgt catccacgat cagcgcccgg
     1261 acccccgaga gctgcggggc ggccgccgtt gcgcgcgggc tcgcggcccc catctggagc
     1321 cggacgcggg ccgtgaacgt cgacccctca ccgaggcggc tcgtgacgcc cagcgtgccg
     1381 cccatcaggt tcaccagctc gcggctgatg gccagcccga gcccggtacc gccatagcgt
     1441 cgcgtcgacg acgcgtcgac ctgggtgaac ttgtcgaaga tgtgctcgat ccggtcatcg
     1501 gcgatcccga tcccggtgtc actgaccccg aatcgcagcc gtacctcacc accgcccgac
     1561 tcctcacact ccaggttcag cagaacatgg cccgtggccg tgaacttgat ggcgttgttg
     1621 gtcaggttga tcaggatctg ccggatccgg cccggatccc cgacgacgta acgcgggaca
     1681 tcgggggcga cccgaatgat gagatcgagt cccttctcgg ccgccgcgcc cgaaagcagc
     1741 tcgccgactt cctccaccgc gatcatgatg tcgaacgaca ccggctccag ccgaaggcga
     1801 ccggcctcga tcttcgaaaa gtcgaggatg tcgttgatga tcgtcagcag cgcatccgcc
     1861 gacttccgca ccgtctcggc gtggtgccgc tggcgcgcgg tcagctcggt gtcgagcagc
     1921 agtccggtca tgccgatgat cccgttcatc ggggtccgaa tctcgtggct catccgggcc
     1981 aggaactccg acttgagccg cgaggcttcc agagcccgat cgcgagcggc cgcgagctcg
     2041 gcctccacct gcttccgctg ggtgatgtcc cggaggacgc ccgtgaagaa gctacggccc
     2101 cgcgcctcga agcgcgagaa cgacgtctcc agcggcacct cccgcccgtc ctgcgtcaac
     2161 ccgaccaggt cgatatttcg ccagctgcgg aaccggcgct cgccgctctt gagggaccgg
     2221 tggaggtcgg cccgatgcgc ctcgtggaag cgctcgggaa tcaggcgcgt cagaggctcc
     2281 ccggccatct cggccgcggc gtagccgaac agccgctcgg cggctcggtt gaactcgcgg
     2341 accgctccct ccgcgtcgat cgacacgatc gcgtcggcgg ccgactcgat gacgtaccgg
     2401 gacttctcct cgctctcgcg cagggtctcc tcggcctcac ggcgcttgag cagatcctcc
     2461 tcctggacgc gccgcgtctc ctcgaggatc gcgaccgact ggcgctggat ctggcagagg
     2521 cgcgtcgtga acagcgccat caccccgacg ggcaggccgt gcgccacgat cgcgaaatcg
     2581 ttccggccgt agaagtagac ccagttcggc tggagatagt tgaggacggc gtggtgcagc
     2641 gcggtcagca ccaggacgag ccagccgagc cgccagtccg agtacatcac gaggagagcc
     2701 atgatcatga agaagtggaa gtgcatctcg atcgagccgc cgctgatgaa cacgaacagg
     2761 taggaatagg cggtgaagga gacgctgagc acgacccgcc aggcgtagtg gttgcgcatg
     2821 cggccgtaca gcccggcggg aatcacgctg gccaggaggc cgagcccgat ggtcaccgcg
     2881 ccctcctcga gcgtggggac ccgccacgag aagggggacg ggaagtagag tc
//
LOCUS       DATMCV010000290         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_149674, whole genome shotgun sequence.
ACCESSION   DATMCV010000290 DATMCV010000000
VERSION     DATMCV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149674"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VNU03_06685"
     CDS             complement(<1..462)
                     /locus_tag="VNU03_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWP74180.1"
                     /translation="MTIREIQADHYRIPLPVALSDSTHGIIEGFELVTVRLRDRDGAE
                     GVGYTYTVGTGGAAVHALIARDLAPRLAGREAEDIEALWQTMWWALHYGGRGGAQALA
                     ISAVDIALWDLRARRQGTPLWRALGGFDPRVPCYAGGIDLEFPLDALLRQTD"
     gene            complement(459..1250)
                     /locus_tag="VNU03_06690"
     CDS             complement(459..1250)
                     /locus_tag="VNU03_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74181.1"
                     /translation="MLAAKIAASAFFVLGGTAAAEWLGTRLGSILGSAPQLAVLSLIF
                     FSIEQGPAFAAESAFWTIPGMGAAVPVYLGYLLATRLVPAPRAWSVAAGVSLGTATFV
                     VATLVLSTIPLGPLTAMPFAAVVCLAAAFLVRRLPDTAPLKRGPISMSLLAIRIAVSA
                     LTVLAVTSVAHALGPKWSGLVVGFPVNGLPVMALLHARYGTAVIMPFIRMFPVGAFGI
                     CIFNLVASRTLVRIGLPATIALAYAVDVAYLAAVAWLRRPRPVSP"
     gene            complement(1271..>2106)
                     /locus_tag="VNU03_06695"
     CDS             complement(1271..>2106)
                     /locus_tag="VNU03_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74182.1"
                     /translation="GHGGFMGIGAYVTALAWNHGAVSPWIGIPVALGAGGVLAVLVAY
                     PCFRFRITGHYFALVTLALSGIVLQVITATRDHTGGSLGYTPTRYQGGSSLYALQFTG
                     KTTWYLIALSVWAAGLYAWHRVDRSMTRYALEAISEDEDGAAAAGVHVTAEKLKITVL
                     SAMMTALAGALYCQYQMFISPDTVSGISISLQMVFAVVVGGIYVSLGPTVGAVITILL
                     AEVLRISFGTRAVGWDNLVYGVLLVLFIVFLPKGILGSLLDLRRRAAPAAPPRTQTGA
                     A"
BASE COUNT          361 a          744 c          775 g          226 t
ORIGIN      
        1 gtcggtctgg cgcaacagag cgtcgagcgg gaactccagg tcgatcccgc ccgcatagca
       61 cggcacccgc gggtcgaagc cgccgagcgc gcgccagagc ggcgttccct gccggcgcgc
      121 ccgcaggtcc cagagcgcga tgtccaccgc ggagatggcg agggcctggg cgccgccgcg
      181 gccgccgtag tggagcgccc accacatggt ctgccagagc gcctcgatgt cctccgcctc
      241 ccggccggcc aggcgcgggg ccaggtcgcg cgcgatcagc gcgtgcaccg cggcgccccc
      301 ggttcccacc gtgtaggtgt agccgacccc ctcggcgccg tcgcgatcgc gcaggcgaac
      361 cgtgaccagc tcgaagccct cgatgatgcc gtgggtggag tcggagaggg ccaccggcag
      421 cgggatgcga tagtggtcgg cctggatctc gcggatcgtc atgggctcac cgggcgcggg
      481 cggcgcagcc aggccaccgc cgcgaggtag gcgacgtcca ccgcgtacgc gagcgcgatc
      541 gtagcgggca gcccgatccg cacgagggtg cgtgacgcca ccaggttgaa gatgcagatg
      601 ccgaaggcgc ccaccgggaa catccggatg aacggcatga tcaccgcggt cccgtagcga
      661 gcgtgcagca gcgccatcac cggcaggccg ttgaccggga agcccaccac cagcccggac
      721 cacttggggc cgagcgcgtg ggccaccgag gtgaccgcga gcacggtgag ggccgagacc
      781 gcgatgcgga tcgcgaggag cgacatcgag atggggccgc gcttgagcgg cgcggtatcg
      841 ggcaggcggc gcacgaggaa ggcggcggcc aggcacacca cggccgcgaa cggcatcgcg
      901 gtgagcggcc cgagcgggat cgtgctcagg accagggtcg cgaccacgaa cgtcgcggtg
      961 ccgagcgaca ccccggcggc caccgaccac gcgcggggcg ccggcaccag tcgcgtcgcc
     1021 agcaggtaac cgaggtacac cggcaccgcc gcccccatgc ccgggatggt ccagaaggcg
     1081 ctctccgccg cgaaggcggg cccctgctcg atcgagaaga agatgagcga caggaccgcg
     1141 agctgcggcg ccgagcccag gatggagccg agccgcgtgc ccagccactc ggcggccgcg
     1201 gtgccgccca ggacgaagaa cgccgaggcc gcgatcttcg cggccagcaa gacgagagcg
     1261 gacgagtcga tcaggcggcc ccggtctgcg tgcgaggcgg ggccgccggc gccgcccggc
     1321 ggcgcaggtc gagcaggctc cccaggatgc ccttgggcag gaacacgatg aacaacacga
     1381 gtagcacacc gtagaccagg ttgtcccagc ccaccgcgcg ggtgccgaag gagatgcgga
     1441 gcacctccgc gagcaggatc gtgatcaccg cccccaccgt cgggcccagc gagacgtaga
     1501 tgccgcccac caccaccgcg aagaccatct ggagcgagat cgagatgccg ctcaccgtgt
     1561 ccggggagat gaacatctgg tactggcagt agagcgcgcc ggccagcgcg gtcatcatcg
     1621 cgctcagcac cgtgatcttg agcttctccg cggtcacgtg cacgcccgcc gcggcggcgc
     1681 cgtcctcgtc ctccgagatg gcctcgagcg cgtagcgggt catgctccgg tccacccggt
     1741 gccaggcgta gagcccggcg gcccacaccg agagcgcgat cagataccag gtggtcttgc
     1801 cggtgaactg cagggcgtag agcgagctgc cgccctggta gcgcgtcggg gtgtagccaa
     1861 gcgagccgcc ggtgtggtcg cgcgtggcgg tgatgacctg gagcacgatg ccggacagcg
     1921 cgagggtgac cagggcgaag taatggccgg tgatgcggaa gcggaagcag ggataggcca
     1981 ccagcaccgc cagcacgccc cccgcgccca gggccaccgg aatgccgatc cacgggctca
     2041 ccgcgccgtg gttccaggcc agcgcggtca cgtaggcccc gatgcccatg aacccgccgt
     2101 ggccca
//
LOCUS       DATMCV010000291         2396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_149681, whole genome shotgun sequence.
ACCESSION   DATMCV010000291 DATMCV010000000
VERSION     DATMCV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149681"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VNU03_06700"
     CDS             <1..463
                     /locus_tag="VNU03_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74183.1"
                     /translation="AQALKERQKIGWSDTLMVSTGPLTDETYLGLAGDASDGVEGLAT
                     LPDPLRADQPGVKLYREHLQKYFPKNEPNRYSLAGYVAGMLFAEGAKRAGRNLTRDGL
                     VTALESLKGFDTGILPPVTIGADHDTQKQGFWVRVENGRFKPLSDWLKSEP"
     gene            460..1239
                     /locus_tag="VNU03_06705"
     CDS             460..1239
                     /locus_tag="VNU03_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74184.1"
                     /translation="MTRPSAPLLEIEGLSIRFGGLAALSEVDLAVGAGEIFALIGPNG
                     AGKTTVFNVLTGLYRPSGGRVRFDGHDLLARAPHAVARLGVARTFQNTEVFRGLSALD
                     NVLIGAHARLRGHVIAAALGLPGVRREEARARDRARALLARVGLGDVAGRDAAGLPLG
                     LQKRVELARALALEPRLLLLDEPAGGLNPTETQELMRLIRSLRDDDRLTILLVEHNME
                     LVMGVSDRVAVLQHGRRLAEGTPAEVSRDPAVVAAYLGTDE"
     gene            1236..1979
                     /locus_tag="VNU03_06710"
     CDS             1236..1979
                     /locus_tag="VNU03_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74185.1"
                     /translation="MSVLLGLDGVSVAYGKRRALDGVSLTVAAGEIVTLLGANGSGKS
                     TTLRTISGLVRPSAGRVVFDGRDITRAAPDAIVAGGVGHVPEGREIFPEFTVRENLLV
                     GGHTAPRAAVTEGTERAFALFPVLGQRARQLAGTLSGGEQQMLAIARALMIRPRLLLL
                     DEPSLGLSPRLAREIFDVIARLNAEGTTILLVEQNARRALALASRAYVLETGRVVVSG
                     PARELAADPRIRAAYLGLAEPAAPGAGRV"
     gene            2004..>2396
                     /locus_tag="VNU03_06715"
     CDS             2004..>2396
                     /locus_tag="VNU03_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP74186.1"
                     /translation="MPFVASNGLTEEGTSRTISVGGQAGDQAGKMTVHYHDVGAGDPE
                     LFLHSYGPGTTAWITFHKAIGALAPHFRCILMDLPNFSKTGPIVYREGVHAVQARTAV
                     ALLDALGIERAHWVGSSQGGQSSMVAAIT"
BASE COUNT          321 a          857 c          900 g          318 t
ORIGIN      
        1 cgcccaggcg ctcaaggagc ggcagaagat cggctggagc gataccttga tggtctcgac
       61 gggcccgctg accgacgaga cctacctggg cctggccggc gatgcctccg acggggtgga
      121 ggggctggcg accctgcccg atccgctgcg cgccgatcag ccgggggtca agctctaccg
      181 ggagcacctg cagaagtact tcccgaagaa cgagccgaac cgctactcgc tcgccggcta
      241 cgtggccggc atgctcttcg ccgagggcgc caagcgcgcg gggcgcaatc tcacccgcga
      301 cgggctcgtg accgcgctcg agtcgctcaa gggcttcgac accgggatcc tgccgccggt
      361 cacgatcggg gccgaccacg acacccagaa gcagggattc tgggtccggg tggagaacgg
      421 gcgcttcaag ccgctctcgg actggctgaa gtcggagccg tgacgcggcc gtccgcgccg
      481 ctgctcgaga tcgaagggct ctcgatccgc ttcggcgggc tcgccgcgct gagcgaggtg
      541 gatctggccg tcggggcggg ggagatcttc gcgctcatcg gcccgaacgg ggccggaaag
      601 accaccgtct tcaacgtcct gaccggactc taccggccgt cgggcgggcg cgtgcgcttc
      661 gacggccacg atctgctggc ccgcgccccc cacgcggtgg cgcgcctcgg cgtggcgcgg
      721 accttccaga acacggaggt gttccgcggg ctctccgcgc tcgacaacgt gctgatcggc
      781 gcgcacgcgc ggctgcgggg gcacgtgatc gcggccgcgc tcgggctgcc cggggtgcgc
      841 cgggaggagg cgcgcgcccg cgatcgggcg cgggcgctcc tggcgcgggt ggggctcggg
      901 gacgtcgcgg gccgcgacgc ggcggggctg ccgctcgggc tccagaagcg cgtggagctg
      961 gcgcgggcgc tggccctcga gccgcgcctg ctcctgctcg acgagccggc gggcgggctc
     1021 aacccgaccg agacccagga gctgatgcgc ctgatccgga gcctccgcga cgacgaccgc
     1081 ctgacgatcc tgctcgtcga gcacaacatg gagctggtga tgggcgtctc cgaccgggtg
     1141 gcggtgctcc agcacgggcg acggctcgcc gaggggacgc ccgccgaggt gagccgggat
     1201 cccgcggtgg tggcggccta cctcggcacc gacgaatgag cgtgctgctc ggcctcgacg
     1261 gcgtctcggt ggcctacggc aagcgccgcg cgctggacgg cgtgtcgctc accgtcgcgg
     1321 cgggcgagat cgtgaccctg ctcggcgcca acggcagcgg caagagcacc acgctgcgga
     1381 cgatctcggg actggtgcgc ccgagcgcgg gccgcgtcgt cttcgacggc cgcgacatca
     1441 cccgcgcggc ccccgacgcc atcgtcgccg gcggcgtcgg ccacgtgccg gagggacgcg
     1501 agatcttccc cgagttcacg gtgcgcgaga atctcctggt gggcggccac accgccccgc
     1561 gcgccgcggt gacagaggga accgagcgcg cgttcgcgct cttcccggtg ctcggccagc
     1621 gcgcgcgcca gctcgcgggg acgctgtcgg ggggcgagca gcagatgctc gcgatcgcgc
     1681 gggccctgat gatccggccg cgattgctgc tgctcgacga gccgtcgctg gggttgtcgc
     1741 cgcggctggc ccgcgagatc ttcgacgtca tcgcccggct caacgccgag ggtacgacca
     1801 tcttgctggt cgagcagaac gcgcggcgcg ccctggccct ggcctcgcgc gcctacgtgc
     1861 tcgagaccgg gcgcgtggtg gtcagcgggc cggctcgcga gctggccgcc gacccgcgca
     1921 tccgcgccgc ctacctgggc ctcgccgagc cggccgcccc tggagccggt cgggtctgaa
     1981 ggccccgccc gaaggaggaa gacatgccgt tcgtcgcgtc gaatgggctc accgaggagg
     2041 gcaccagccg gaccatttca gtcgggggcc aagccgggga ccaggccggg aagatgacgg
     2101 tccactatca cgacgtgggc gcgggcgatc cggagctgtt cctgcactcc tacggccccg
     2161 gcaccaccgc gtggatcacg ttccacaagg cgatcggcgc gctcgcgccg cacttccgct
     2221 gcatcctcat ggacctgccg aacttcagca agaccggccc catcgtctac cgggaagggg
     2281 tgcacgcggt ccaggcgcgg accgcggtcg cgctgctgga cgccctcggc atcgagcgag
     2341 cgcactgggt cggcagctcg cagggcgggc agtcgtcgat ggtggcggcc atcacg
//
LOCUS       DATMCV010000292         2676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_214086, whole genome shotgun sequence.
ACCESSION   DATMCV010000292 DATMCV010000000
VERSION     DATMCV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214086"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1539)
                     /locus_tag="VNU03_06720"
     CDS             complement(154..1539)
                     /locus_tag="VNU03_06720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWP74187.1"
                     /translation="MATNVIMPALELAQETGKLLRWIKAPGDTVRKGEPIAEIETDKV
                     TIELEAPASGVLSAVSAQEGDVVPVGHTIAQIAAATEASSTLSPQGRGQGEGPKGEGP
                     KGEGPEAPAPDSRPKASPLARKIAEEHGVDLARVKTASGKIEKADVLAFVESQRVVAA
                     PTNGSPGRLVAASPKARRLAAERGIDLRALKGSGPGGAVLTLDVPAATVLSTTGPLTT
                     GPLTTSPLTLPSPQRGEGGGEGGGVGTVWRIMAERMTASWTSAPHFYLVREVNVTRLA
                     AWLQTARKQTGAHVTYTDLLVKLVGATLAQHPRVNVSWKDGTLERHADINIGLAVALE
                     DGLVVPVLHKADTLGLKEIATRREDLVTRAQSGKLRPADIQGGVFTISNLGMYGVDAF
                     SAIVNPPQAAILAVGRITDRVVPVNGQPAVQPTMVLTLSCDHRALDGARGAQFLGALA
                     DLVEEPLALLV"
     gene            complement(1577..2554)
                     /locus_tag="VNU03_06725"
     CDS             complement(1577..2554)
                     /locus_tag="VNU03_06725"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015500947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWP74188.1"
                     /translation="MRELTYGEAVKEAIAEEMRRDPRVFLIGEDVAEAGHPFKTLVGL
                     VQEFGTDRVIDTPISEPGYAGIGVGAAMTGMRPVVDVMFGDFITLTMDQMVNQAAKAH
                     YMSGGKIKVPIVFRTTLGATRRSAAQHSQSLHAWVSHIPGLKVALPSGPYEAKGLMKT
                     AIRDDSPVVIFEDKMMYRMKGPVPEGDYTIPFGVAEVKRAGKDVTIVATSSMVGVALG
                     AAKMLEDAGISAEVIDPRTTWPLDKQALIESAKKTSRALVVDEGYERYGVTAEMAAVI
                     AEGAFYYLDAPVKRMGAMDVPVPFSPVLEDLTVPSEKTVFEAARTLCRK"
BASE COUNT          363 a          944 c          929 g          440 t
ORIGIN      
        1 cgggatcggc ctggagcgcc tcggccatcc gctcctcgcc ggtggggccg taggacagca
       61 gatcgtccat gcccttctgg aagcgctcga aggcgacggg tgaatcggtc gtgacggtca
      121 ggccgtagcg atcggtgaac gccatgagag tcctcagacg agcagggcca gcggctcctc
      181 gacgagatcc gcgagcgccc cgaggaactg ggcgccccgg gcgccgtcga gggcgcgatg
      241 gtcgcaggac agcgtgagca ccatggtcgg ctgcacggcc ggctggccgt tgaccgggac
      301 gacgcgatcg gtgatgcggc ccaccgccag gatcgcggcc tgcggcgggt tgacgatggc
      361 gctgaacgcg tccacgccgt acatgccgag gttgctgatg gtgaagacgc cgccctggat
      421 gtcggccggc cgcagcttgc cggactgggc ccgcgtcacc aggtcctcgc gacgggtggc
      481 gatctccttg agccccagcg tgtcggcctt gtggagcacc gggacgacga gcccgtcttc
      541 cagcgcgacg gccaggccga tgttgatgtc ggcgtgacgc tcgagcgtgc cgtccttcca
      601 cgacacgttc acgcgcgggt gctgggccag cgtggccccc accagcttga ccagcaggtc
      661 cgtgtaggtg acgtgcgcgc ccgtctgctt gcgcgccgtc tgaagccacg cggccagccg
      721 cgtcacgttg acctcgcgca ccaggtagaa gtgcggcgcg ctcgtccacg acgcggtcat
      781 ccgctccgcc atgatccgcc acaccgtccc cacccctccc ccctcgcctc ccccctctcc
      841 cctctgggga gagggcaggg tgagggggct ggtggtgagg ggccccgtgg tgaggggccc
      901 ggtggtagaa agcacggtgg cggccggcac atccagcgtc agcaccgcgc cccccggccc
      961 cgaccccttc agcgcacgta gatcgatccc gcgctccgcc gccagccgcc gcgccttcgg
     1021 cgacgcggcg acgaggcgcc ccggcgagcc gttcgtcggc gccgcgacca cgcgctggct
     1081 ctcgacgaag gcgagcacgt cggccttctc gatcttgccg ctcgcggtct tgacgcgggc
     1141 gaggtcgacg ccgtgctcct cggcgatctt gcgcgcgagc ggggaggcct tcgggcggga
     1201 gtcgggcgcg ggggcttcgg gcccctcccc tttgggcccc tcacctttgg gcccctcacc
     1261 ctgccctctc ccctgagggg agagggtgga ggaggcttcg gtggcggcgg cgatctgggc
     1321 gatggtgtgg ccgacgggaa ccacgtcgcc ttcctgggcg ctgaccgcgc tcaggacgcc
     1381 ggaggcgggg gcctcgagct cgatcgtgac cttgtcggtc tcgatctcgg cgatgggctc
     1441 gcccttgcgg accgtgtcgc ccggggcctt gatccagcgc aggagcttgc cggtctcctg
     1501 ggccagctcg agcgcgggca tgatgacgtt ggtcgccatc gctaccccct cgcctttggg
     1561 cccctcaccg ttggaattac ttgcggcata gggtgcgggc ggcctcgaag acggtcttct
     1621 cggaggggac cgtgaggtct tccagcacgg gggagaacgg caccggcacg tccatggcgc
     1681 ccatgcgctt gaccggcgcg tccaggtagt agaaggcgcc ctccgcgatg accgccgcca
     1741 tctcggcggt gacgccgtag cgctcgtagc cctcgtccac caccagcgcg cgcgaggtct
     1801 tcttggccga ctcgatcagc gcctgcttgt cgagcggcca ggtggtgcgc gggtcgatca
     1861 cctccgcgct gatgcccgcg tcctccagca tcttggccgc gccgagcgcc acgcccacca
     1921 tgctgctggt ggcgacgatg gtgacgtcct tgcccgcgcg cttcacctcg gcgacgccga
     1981 acgggatcgt gtagtcgccc tccggcaccg ggcccttcat ccggtacatc atcttgtcct
     2041 cgaagatgac caccgggctg tcgtcgcgga tggcggtctt catgagcccc ttggcctcgt
     2101 agggaccgga gggcagcgcc accttgaggc cggggatgtg gctcacccag gcgtggagcg
     2161 actgcgagtg ctgggcggcc gagcggcggg tggccccgag cgtggtgcgg aagacgatcg
     2221 gcaccttgat cttgccgccc gacatgtagt gcgccttggc ggcctggttc accatctggt
     2281 ccatggtcag ggtgatgaag tcgccgaaca tcacgtccac caccgggcgc atgccggtca
     2341 tggccgcgcc gacgccgatg cccgcgtagc cgggctcgga gatcggcgtg tcgatgacgc
     2401 gatccgtccc gaactcctgc acgagcccga ccagtgtctt gaacggatgc cccgcctcgg
     2461 ccacgtcctc gccgatgagg aacacgcggg ggtcccgccg catctcctcc gcgatcgctt
     2521 ccttgaccgc ctcgccgtag gtcagctctc gcaccgccgt ctgaaccgtc gacatcgtca
     2581 tccacctcgc ggacgctgtg ataggcaaga ggtatctgta tcacatctca ggtgcaatcg
     2641 acggcctcct cagtaatgaa actccgcgat ggagat
//
LOCUS       DATMCV010000293         4315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_14154, whole genome shotgun sequence.
ACCESSION   DATMCV010000293 DATMCV010000000
VERSION     DATMCV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14154"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VNU03_06730"
     CDS             <1..984
                     /locus_tag="VNU03_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP74189.1"
                     /translation="ETGMTFDTKYLWTLPMFHCNGWCFTWAVTAVAGTHVCLRKVDPG
                     RIWDLIDAEGITHYNGAPTVQIGVVNDPKAHTLSRPVTVTVAGAPPSPTLLGKLKELG
                     FRPVHVYGLTETYGPHTVGAWNADWDALPVEQQASLAARQGQGYALFDLVRVVDGEMN
                     DVPRDGETLGEVVMVGNNVAKGYYEQPDATAEAFRGGWFHSGDIGVWHPDGYVELRDR
                     KKDIIISGGENISTIEVEQCVARHPAVMECAVVAIPDEKWGERPKAFVTLKPGQHATE
                     AEIIDFCKRHIAGFKAPAAVDFGELPKTSTGKIQKFVLRDREWKGKAKRIN"
     gene            989..1996
                     /locus_tag="VNU03_06735"
     CDS             989..1996
                     /locus_tag="VNU03_06735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019914932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP74190.1"
                     /translation="MRIGVLDQSPVRSGGTPADAIHETLELARAADRWGYHRYWLAEH
                     HSTPGLAGASPEVLITQVAAVTSRIRVGSGGVMLPHYSALKVAEQFRMLETLHPGRID
                     LGIGRAPGSDQRTARILRAGSGALGAEQYPRQVQDLVAFLHDDLPAEHPWRGVHAMPA
                     GPSIPELWMLGSSDDSALLAAHLGQAFCFAHFINEQGGAEVTRAYRGRFCASPDVRAP
                     RSSVAVFAVCAETEEQARRLARSRDLFIVRLYTGRPAPYPSVEEAERYPYNAQELAIA
                     QHAARRSVVGTPEQVRERLLAMVAEYQADELIVVTITHDFKARLRSYELLAEAFDLAR
                     Q"
     gene            2006..2809
                     /locus_tag="VNU03_06740"
     CDS             2006..2809
                     /locus_tag="VNU03_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWP74191.1"
                     /translation="MTMRLSLHTWTLDTTPLSEALAVIKKTGWDAVELRRLDFERAAQ
                     AGQSAEQVLALVRASGLPVACVGVELGWLWATGEERARLLQVFAEQAARAKALGCATI
                     MSPVDRGRGDAADAARSIREVGDIAREHGVRLAVEFNSQCEQLNALGPMREVMARAGH
                     PQTGLLLDTYHLGRTGASPREIADVRPEEIAYVQYSDVPRTGLEPGKALDRLPPGQGS
                     VPFKEFFAAFTRQGYTGYASYEAPNPAAWKRPAEDVAREALAATRAVMP"
     gene            2806..>4315
                     /locus_tag="VNU03_06745"
     CDS             2806..>4315
                     /locus_tag="VNU03_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74192.1"
                     /translation="MTRRGWLVAGLLLLTIGRTAPVAAASEGELTWAVHVSLAPTWFD
                     PAETPGVIVPFMVLYALHDALAKPMPGAPMAPSLAESWSVSPDGRVYDFVLRAGVRFH
                     NGDPVTAEDVKFSLERYRGGAAATLKARVAGVDAVDARHVRIRLKQPWPDFMTFYATP
                     ATGAAWIVPKRYVERVGEDGFKKAPIGAGPYRFVSFTPGIELVLEAFDGYWRKVPAVK
                     RLVFRSVPDESTRLAMLKRGEADIAYAFRGPLAEELRRTPGLTLKATSPTFTEWLTFT
                     EQWDPKSPWADRRVRQATNLAIDRKSINEAEYLGYGRVSTSIIPHDFQFYWPAPAPPY
                     DPARARQLLAEAGYPRGFDAGEIASDLTYAPTVEAVINNLQPVGIRARLRALERAAYL
                     RADQEKGFKRVARIGSAAAGNAATRIEAFVITDGIRSYGGYPEIDALYREQAGERDAK
                     KREALLHRIQQAMHERVMFAPIVEPAFLNGIGPRVDEAGLGLITGHLYSAPYE"
BASE COUNT          635 a         1556 c         1545 g          579 t
ORIGIN      
        1 gagaccggca tgaccttcga cacgaagtat ctctggaccc tgcccatgtt ccactgcaac
       61 ggatggtgct tcacgtgggc ggtcaccgcg gtggccggca cccacgtctg tctccggaag
      121 gtcgacccgg gccgcatctg ggacctgatc gacgcggagg gcatcaccca ctacaatggc
      181 gcgcccaccg tgcagatcgg cgtggtcaac gacccgaagg cccacacgct ctcgcgcccg
      241 gtgacggtga cggtggcggg cgccccgccg tcgcccacgc tcctcggcaa gctcaaggag
      301 ctcggcttcc ggccggtcca cgtctacggc ctcaccgaga cctacgggcc ccacaccgtc
      361 ggcgcgtgga acgcggactg ggacgcgctg ccggtcgagc agcaggcgag cctggccgcg
      421 cgccagggcc agggctacgc gctcttcgac ctcgtccggg tggtggacgg cgagatgaac
      481 gacgtgcccc gcgacggcga gaccctgggc gaggtggtga tggtcggcaa caacgtggcc
      541 aagggctatt acgagcagcc ggatgcgacc gcggaggctt tccggggcgg ctggttccac
      601 tcgggcgaca tcggggtctg gcatcccgac ggctacgtcg agctgcgcga ccgcaagaag
      661 gacatcatca tctcgggagg cgagaacatc tcgaccatcg aggtcgagca gtgcgtggcc
      721 cggcaccccg cggtgatgga gtgcgcggtg gtcgccatcc cggatgaaaa atggggcgag
      781 cggcccaagg ccttcgtcac cctcaagccg ggccagcacg cgaccgaggc ggagatcatc
      841 gacttctgca agcggcacat cgcgggcttc aaggcgcccg ccgcggtcga cttcggcgag
      901 ctgcccaaga cctcaaccgg caagatccag aagttcgtcc tgcgcgaccg ggagtggaag
      961 ggcaaggcga agcggatcaa ctagccccat gcgcatcggg gtcctcgatc agtcgccggt
     1021 gcgcagcggg ggcactcccg ccgacgccat ccacgagacg ctcgagctgg cgcgggccgc
     1081 ggatcgctgg ggttatcatc gctactggct cgccgagcac cactcgaccc ccggcctcgc
     1141 gggggcgagc cccgaggtgc tgatcaccca ggtggccgcg gtgacgagcc gcatccgggt
     1201 cggctcgggc ggcgtgatgc tgccacacta cagcgcgctc aaggtggccg agcagttccg
     1261 catgctcgag acgctccacc ccggccgcat cgatctcggg atcggccgcg cgccgggcag
     1321 cgaccagcgg acggcccgga tcctccgggc cggatccggc gccctcgggg ccgagcagta
     1381 tccgcggcag gtgcaggacc tggtggcgtt cctccacgac gacctgcccg ccgagcatcc
     1441 ctggcgcggc gtgcacgcca tgcccgcggg cccgagcatc cccgagctgt ggatgctggg
     1501 ctcgagcgac gacagcgcgc tgctcgcggc ccatctcggg caggccttct gcttcgccca
     1561 cttcatcaac gagcaggggg gcgcggaggt cacgcgcgcc taccgcgggc ggttctgcgc
     1621 ttcgcccgat gttcgcgcgc cgcgcagcag cgtcgccgtc ttcgcggtct gcgcggaaac
     1681 ggaagagcag gcgcggcgcc tggcccggag ccgcgacctc ttcatcgtgc gcctctacac
     1741 cgggcggccc gcgccgtacc cgtcggtgga ggaggcggag cgctatccct acaacgccca
     1801 ggagctcgcg atcgcccagc acgcggcccg tcgcagcgtg gtgggcaccc ccgagcaggt
     1861 gcgcgagcgc ctcctggcga tggtcgcgga ataccaggcc gacgagctga tcgtggtcac
     1921 gatcacccac gacttcaagg cgcgcctgcg ctcctacgaa ttgctggccg aggcgttcga
     1981 tctagcgcgg caataggaga ccatcatgac catgcgactg tcgctgcaca cctggacgct
     2041 cgacacgacg ccgctgtcgg aggccctcgc ggtcatcaag aagacgggct gggacgcggt
     2101 cgagctgcgc cgcctcgact tcgagcgggc ggcccaggcc ggccagtccg ccgagcaggt
     2161 gctggccctc gtgcgcgcct ccgggctgcc ggtggcctgc gtgggcgtgg agctcggctg
     2221 gctgtgggcc accggcgagg agcgcgcccg gctcctgcag gtgttcgccg agcaggccgc
     2281 gcgcgcgaag gcgctcggct gcgccaccat catgagcccg gtggatcgcg ggcgcgggga
     2341 cgcggccgac gcggcgcgga gcattcgcga ggtcggcgac atcgcgcgcg agcacggagt
     2401 gcgcctggcc gtcgagttca actcccagtg cgagcagctc aacgccctgg gcccgatgcg
     2461 cgaggtgatg gcgcgggccg gccatcctca gaccgggctc ctgctcgaca cgtaccacct
     2521 cgggcgcacc ggggcctccc cgcgcgagat cgcggacgtg cggccggagg agatcgccta
     2581 cgtgcagtac agcgacgtgc cacgcaccgg cctcgagccg ggcaaggcgc tcgaccgcct
     2641 gccgccgggg cagggaagcg tgccgttcaa ggagttcttc gccgcgttca cccggcaggg
     2701 ctacaccggc tacgcgagct acgaggcgcc caaccccgcc gcgtggaagc ggccggccga
     2761 ggacgtggcg cgcgaggcgc tcgccgcgac gcgcgccgtg atgccgtgac gcggcggggc
     2821 tggctggtcg ccggcctcct gctgctgacg atcggccgca ccgcccccgt cgccgccgcg
     2881 tcggagggcg agctgacctg ggcggtgcac gtctcgctcg cgcccacgtg gttcgacccc
     2941 gcggagacgc ccggggtcat cgtgccgttc atggtcctct acgcgctgca cgacgccctc
     3001 gccaagccga tgccgggtgc cccgatggcg ccgagcctgg ccgagtcgtg gagcgtctcg
     3061 cccgacgggc gagtctacga cttcgtgctc cgcgccgggg tgcgcttcca caacggcgac
     3121 ccggtcaccg cggaggacgt gaagttctcc ctcgagcgct accggggcgg agcggcggcc
     3181 accctcaagg cgcgggtggc cggggtggac gcggtcgacg cccgccacgt gcgcatccgc
     3241 ctcaagcagc cctggccgga cttcatgacg ttctacgcga cgcccgcgac cggggccgcg
     3301 tggatcgtcc ccaagcgcta cgtggagcgg gtgggcgagg acggcttcaa gaaggcgccg
     3361 atcggcgcgg ggccgtaccg cttcgtctcg ttcacccccg ggatcgagct ggtgctcgag
     3421 gcgttcgacg gctactggcg caaggtacct gcggtgaagc gcctggtgtt ccgctcggtc
     3481 ccggacgagt cgacgcgcct ggccatgctc aagcggggcg aggccgacat cgcctacgcg
     3541 ttccgcggtc cgctcgcgga ggagctgcgc cggactccgg ggctcaccct caaggcgacc
     3601 tcgcccacct tcaccgagtg gctgaccttc accgagcagt gggatccgaa gtctccgtgg
     3661 gccgaccgcc gggtgcgcca ggccacgaac ctcgccatcg atcggaagtc gatcaacgag
     3721 gccgagtacc tgggctacgg acgggtcagc acgagcatca tcccgcacga cttccagttc
     3781 tactggccgg cgcccgcgcc tccctacgat ccggcccggg cccggcagct cctcgccgag
     3841 gccggctatc cgcggggctt cgacgcgggc gagatcgcct ccgacctcac ctacgcgccc
     3901 acggtggagg cggtcatcaa caacctgcag ccggtcggca tccgggcgcg gctgcgcgcg
     3961 ctggagcggg ccgcctacct gcgggccgac caggagaagg ggttcaagcg ggtcgcgcgc
     4021 atcggcagcg cggcggcggg caacgcggcg acccgcatcg aggccttcgt gatcaccgac
     4081 ggcatccggt cctacggcgg ctaccccgag atcgacgcgc tgtaccgcga gcaggcgggc
     4141 gagcgcgacg cgaagaagcg ggaggcgctc ctgcatcgca tccagcaggc gatgcacgag
     4201 cgggtcatgt tcgccccgat cgtggagccc gccttcctca acgggatcgg gccgcgggtg
     4261 gacgaggcgg gtctcggcct gatcaccggc cacctctact ccgcgcccta cgagg
//
LOCUS       DATMCV010000294         9656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_880832, whole genome shotgun sequence.
ACCESSION   DATMCV010000294 DATMCV010000000
VERSION     DATMCV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880832"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1263)
                     /gene="lpxK"
                     /locus_tag="VNU03_06750"
     CDS             complement(139..1263)
                     /gene="lpxK"
                     /locus_tag="VNU03_06750"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWP74193.1"
                     /translation="MSTPRRAPEVLLRRYWEDPDTPVVTAGLSVLSWAYRAAIAVRDR
                     AYRWGVLPTGRLPCPVVSVGNLTLGGSGKTPTVELAVRTLLDMGAIPAVLSRGYGRVT
                     RGVHVVADRDGIRAEPRSAGDEPLLLAERLPGVPVVVGENRYEAGRVAVERCGATALV
                     LDDGFQHRTLAKDLEIVVVPGRAPWGNARVFPRGMLREPLSGLGRAHVVVVTNPSGPD
                     TVDEVTATVRRHHATAAVLAARYQLQDAIETQRHRRIPVAELAGRRLLAFAGLGSPQG
                     FADTLDAAGIRRVGFAEFPDHHWFTAGDLAELARDARAVGAQGLVTTEKDWVRVRDLP
                     PPPLPLWVLPVRLVIESGQETWQRLLAAVLAPTALGRPCS"
     gene            complement(1260..2549)
                     /locus_tag="VNU03_06755"
     CDS             complement(1260..2549)
                     /locus_tag="VNU03_06755"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HWP74194.1"
                     /translation="MYALYSALLRLGLLAYLPAFLVRRRRAGYGHDLPQRLGRLGDGL
                     PAEPRGWVHAVSVGESAAAVPLVEAIHRRWPELGIVVSTITPTGARIVRDRLAGTAVH
                     RYFPLDLPGPVRRALDAARPRFFIAIETELWPNFLRALARRRIPSMIANGRISDRSFH
                     RYLRVRWLMRRVLADVTVFAMQSEEDARRILALGAPPSRVVVTGNLKSDLVPEAELVD
                     AAWRERLGLGATDRLWIAGSTHRGEEAIVLDVFLRARARCPGLGLLLAPRHPERAGEV
                     EDLIRERGLVPVRRSRLPDGHAPGAVVILDTVGELAALYGVAEVVFVGGSLVPIGGHN
                     VLEPAMRGKPVLYGPHTSNFREGAERLQRSGGGIVVKDGLELERELSLLLEDPELARR
                     TGDAARAAFAGRQGAVHATLELIALHLWPARGSSPTP"
     gene            complement(2549..3202)
                     /locus_tag="VNU03_06760"
     CDS             complement(2549..3202)
                     /locus_tag="VNU03_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWP74195.1"
                     /translation="MKIPAPLALAVGPALGASALRALALTLHVRREEAAVMPCWTARA
                     PVIYAVWHGRMLLLPLFYGRRGSHALASRSRDGELVARWISRFGLVPVRGSSTRGGGE
                     ALRQLARVLRQGGEVVVVPDGPTGPREVLKAGVIALARLSGAPIVPTAVGASREWRLR
                     SWDEFRIPKPFARCVIRFGEPIRVPADGDRAALEAARKDVESALRSLTWQVDEEARA"
     gene            complement(3199..4392)
                     /gene="lpxB"
                     /locus_tag="VNU03_06765"
     CDS             complement(3199..4392)
                     /gene="lpxB"
                     /locus_tag="VNU03_06765"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWP74196.1"
                     /translation="MAAAEPATSRAPVMLSAGEASGDLHGGMLCRALREMEPGVRLVG
                     MGGGHMRAAGMDVVVDPTEHAAVGTSEAVGRIPALYRAYRALGARLHVERPRALVLID
                     FPEFNLRLARRARRAGVPVVYFIPPQLWAWRQGRVRQMARRVSRVLAVFPFEPALYER
                     AGVPVEFVGHPLLDVLPLDLTHDEARRRLPADPGHSLVGLLPGSRREEVERLLPPMLE
                     AARRLAAADGRRRFVLGLAPTVPLEQVQAHLRHGGPGPAIEIVAGRTYEVMAAADALL
                     IASGTATLEAALLGTPMVLCYRVSRTTEVIARLLARVRWIGLPNLVSGRLVVPELIQA
                     QVTGERLAAEATRLLDDPVAATAQRAALKELRARLGEPGVGRRAARAVLATARAAPQA
                     APHAR"
     gene            complement(4379..5185)
                     /gene="lpxI"
                     /locus_tag="VNU03_06770"
     CDS             complement(4379..5185)
                     /gene="lpxI"
                     /locus_tag="VNU03_06770"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HWP74197.1"
                     /translation="MERSLGLMAGAGTLPGRAALEARRHGWRVVAFAFEEAPGLADSA
                     DHVIPSAITDIQAVLAGLAAQRVEAAVFVGKFWKSSAFSQYDQADAAGRGLARGGLSD
                     GALAQMVVATLSGMGIDVLDQREFLAPWLLPAGTLSARAPSPEEWDEIREGFRLAGRL
                     ADDGIGQTVVRARGVTVAVEAAEGTDETIRRGGRLAGPGAVVVKTVAASHDYRFDVPT
                     IGTATLEAMAESGATALAVPAGRVLLVDREPVVRLADRSGIALVSVDGGR"
     gene            complement(5164..6195)
                     /locus_tag="VNU03_06775"
     CDS             complement(5164..6195)
                     /locus_tag="VNU03_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWP74198.1"
                     /translation="MTNGTEPKIRGAVVGAGHMGQYHILALAEIWGVELVGVVDTDFA
                     RAQQVAAPYGARAFRDHRELAGLVDFATVAVPTEQHFAVARDLLEAGAHVLVEKPMTS
                     TLEQAKELFRLARQQRRVLHVGHVERFNGAVQELRKIVERPILIESRRLGPFVPRVQD
                     DSVVMDLMIHDIDIVLGLVDGEPRKITAVGSSVHSGATDVANVQIVFDSGAMATITAS
                     RATEEKIRTLAITQPDAYILLDYSHQDIQIHRRAAQEYTLNRESIRYRQASFVEHLLV
                     HKDNPLKLEIRHLVSAVRAADSSGPVELPEADDLRSLAVALEIERMIREGRGETAWPE
                     DLPWSVRSA"
     gene            complement(6192..7016)
                     /gene="lpxA"
                     /locus_tag="VNU03_06780"
     CDS             complement(6192..7016)
                     /gene="lpxA"
                     /locus_tag="VNU03_06780"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWP74199.1"
                     /translation="MIHPTAVVDRGAELAADVRVGPYSVIGPEVTVAAGAEIGAHAVL
                     EGRVRVGARCRVGHGAIIGGVPQDLKFREGTPVGVSLGDDTVVREYVTIHRATHEGHD
                     TTVGRHCLVMASSHIAHDCVLGDHIIMINYAGLTGHITVEDRVTIGGLTGIHPFSRIG
                     TYAYIGGCSKVTQDVPPFIMADGIPAVARSVNVVGMRRGGIDSASRLKVRAAFRLLYR
                     SGMAPGAAIKHVKQELGGHPLVDRLVEFVEASKRGVVPGADRTGRARLDAVEEEIS"
     gene            complement(7048..8088)
                     /gene="lpxD"
                     /locus_tag="VNU03_06785"
     CDS             complement(7048..8088)
                     /gene="lpxD"
                     /locus_tag="VNU03_06785"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWP74200.1"
                     /translation="MSDGVSFTLGRLADALGASLHGDSGRVVRGVAPLDVAGPDQVSF
                     LTDRKYLKQAQASRAGALLVARDVTGLPGALLQVDHPQQALIALLGLFHPEPGAEPGV
                     HPTACVAADARVDRSASIGPLAVVEAEAVIGPRSRVGALSFVGRGAVLGEDVRLYPHV
                     VVREDVRIGNRVIVHSGAVLGADGFGYAFDGQAHRKIPQVGGLRIEDDVEIGANTAID
                     RATLGETIVRRGSKIDNLVQIGHNCDVGEDVILVSQVGIAGSSRVGNRAMLAGQVGVA
                     DHVTIGAGAILTAKSGVPNDVPAGEVWSGIPSRPTSEQKRIWAGESQLPELIKRVRAL
                     EKRLRELEERRS"
     gene            complement(8089..8667)
                     /locus_tag="VNU03_06790"
     CDS             complement(8089..8667)
                     /locus_tag="VNU03_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HWP74201.1"
                     /translation="MRQWMAGWWTAATLTLLTSVGGAMWAHAQAQTPAPSPASNGRVA
                     IVDIQRILARSVAGAAAREQLEKDKTAMQRQLDGQKVELEKMRDELEKKGQLLSADAR
                     REKQDALERKVRDVRRLVDDLQAQLQKKEDALLQKVLQDVAGLIQRLGKEKGFAVVVE
                     RQRAGVLYASTDADLTDDVLRAYDDQTKKATK"
     gene            complement(8683..>9656)
                     /locus_tag="VNU03_06795"
     CDS             complement(8683..>9656)
                     /locus_tag="VNU03_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HWP74202.1"
                     /translation="IGGGYSSQDGVLGTLDLSQRNFLGKGWEVFLRLRGGQNIQTGTI
                     GFTEPWLFDQPLAAGFDIFNTRRLLPDYTVNSLGGDIRLGHPIGEYSRWNALYRVSQD
                     KISDVNPLGSPELISQEGTHLTSLVGLNLSRDTRDNVYDPTRGSTVSIGVDFAGVGFG
                     EKFARSVFSTTYFQPMPWLDHVLSFRLMAGYSFGWSKDPVPLFERFYLGGSNSLRQFK
                     SLQVSPKDNTGTRIGGNSEALGTVEYQIPLFFGIKAAVFYDVGQVWGPDISAGTKIDL
                     SDLRHGVGAGLRWNSPFGPIRIDYGIKLDQKKGESFGNFNFSAGSSF"
BASE COUNT         1331 a         3487 c         3593 g         1245 t
ORIGIN      
        1 gcccgatcaa ggtgatgcgc gccgctcccg cggcccggcg cagtgcggtc agcatgggca
       61 gcgccatcac ggtgtccccg agccagttcg gggtccggac cacgacgtgg ccggcgcgcg
      121 agtcgagggc gagcccgctc acgagcacgg tctccccagc gcggtgggcg ccaggaccgc
      181 ggcgagcaga cgctgccatg tctcctgccc ggactcgatc acgagccgga ctggcagcac
      241 ccagagcggc aggggcggcg gcggcaggtc gcgcacgcgc acccagtcct tctcggtggt
      301 gaccagtccc tgcgccccga ccgcgcgcgc gtcgcgagcg agctcggcga ggtcgccggc
      361 ggtgaaccag tggtggtccg ggaactccgc gaagccgacc cggcggatcc cggccgcgtc
      421 gagcgtgtcg gcgaatccct ggggcgagcc cagccccgcg aaggccagca ggcggcggcc
      481 ggccagctcg gccaccggga tccgccggtg gcgctgcgtc tcgatcgcgt cctggagctg
      541 gtagcgcgcg gccagcaccg cggcggtcgc gtggtgacga cgcaccgtgg cggtcacctc
      601 gtccaccgtg tccggcccgg agggattggt caccaccacc acgtgggcgc gcccgagccc
      661 cgagagcggc tcgcgcagca tgccgcgggg gaagacgcgg gcgttgcccc acggcgcgcg
      721 ccccggcacg accacgatct cgaggtcctt ggcgagcgtg cggtgctgga agccgtcgtc
      781 gagcacgagc gcggtggcac cgcagcgctc caccgcgacc cgtccggctt cgtaccggtt
      841 ctcgcccacc accaccggca ccccgggcag gcgctcggcg agcaggagcg gctcgtcgcc
      901 cgcgctgcgc ggctcggcgc ggatcccgtc gcgatcggcc accacgtgca cgcctcgggt
      961 gacgcggccg tagccccggc tcaggaccgc ggggatcgcg cccatgtcga gcagggtgcg
     1021 caccgccagc tccacggtcg gggtcttgcc gcttccgccg agggtcaggt tgccgaccga
     1081 gaccaccggg cacggcagcc gtccggtcgg cagcacgccc cagcggtacg cgcggtcgcg
     1141 caccgcgatg gcggcgcgat aggcccagct gagcacggag agcccggcgg tcaccaccgg
     1201 cgtgtccggg tcttcccagt agcggcggag caggacctcg ggggcgcggc gcggcgtgct
     1261 catggggtgg gcgacgatcc gcgcgcgggc cagaggtgga gggcgatcag ctcgagcgtg
     1321 gcgtggaccg ccccctgccg acccgcgaac gcggcgcggg ccgcgtcgcc ggtgcgccgg
     1381 gcgagctccg gatcctcgag caggagcgag agctcgcgct ccagctcgag gccgtccttg
     1441 accacgatgc cgccgccgct ccgctgcagc cgctcggcgc cctcgcgaaa gttgctcgtg
     1501 tgcggcccgt acagcaccgg cttgccccgc atcgcgggct ccaggacgtt gtgtccgccg
     1561 atcggcacga ggcttccgcc gacgaacacg acctcggcca ccccgtagag ggcggcgagc
     1621 tccccgaccg tgtcgaggat gacgacggcg ccgggcgcgt gcccgtccgg cagccggctc
     1681 cgccggaccg ggacgagccc gcgctcgcgg atcaggtcct cgacctcgcc ggcgcgctcg
     1741 gggtggcgcg gggccagcag caggccgagg ccggggcacc gtgcccgcgc ccggaggaag
     1801 acgtcgagga cgatcgcctc ctcgccccgg tgcgtgctgc ccgcgatcca gagccgatcg
     1861 gtcgcgccga gcccgagccg ctcgcgccag gccgcgtcga ccagttccgc ctcggggacg
     1921 agatcgctct tgagattgcc ggtgaccacc acgcgcgagg gcggcgcgcc cagggcgagg
     1981 atccggcggg cgtcctcctc gctctgcatc gcgaacacgg tgacgtcggc gagcacccgc
     2041 cgcatgagcc agcgcactcg gaggtatcgg tggaaggagc ggtccgagat gcggccgttg
     2101 gcgatcatgg acgggatgcg ccggcgcgcg agcgcgcgca ggaagttggg ccagagctcg
     2161 gtctcgatcg cgatgaagaa gcgcgggcgc gcggcgtcga gggcgcggcg caccgggccg
     2221 ggcaggtcga gcgggaagta gcggtgcacc gcggtgcccg cgagccggtc cctcacgatc
     2281 cgcgcgccgg tcggggtgat ggtgctgacc acgatgccga gctccggcca gcggcggtgg
     2341 atggcctcga ccagcggcac cgccgcggcc gactccccca ccgagaccgc gtgcacccag
     2401 ccgcgcggct ccgcgggcaa accgtcgccg agccgcccga ggcgctgcgg gagatcgtgg
     2461 ccgtagccgg cgcggcggcg gcgcacgagg aaggccggca ggtacgcgag gaggccgagc
     2521 cggaggaggg cggagtagag cgcgtacatc aggcgcgggc ctcctcgtcc acctgccagg
     2581 tcaggctgcg gagcgcggat tcaacgtcct tccgggcggc ctcgagggcg gcgcggtcgc
     2641 cgtcggcggg gacccggatg ggctcgccga agcggatcac gcagcgggcg aagggcttcg
     2701 ggatgcggaa ctcgtcccag gagcgcaggc gccactcgcg cgaggccccg accgccgtcg
     2761 gcacgatcgg cgcgccggag aggcgggcca gcgcgatcac gccggccttg aggacctcgc
     2821 gcgggccggt ggggccgtct ggaacgacca ccacctcgcc cccctgccgg agcacccgcg
     2881 cgagctgacg cagggcctcg ccgccccctc gcgtggagga gccgcgcacc gggacgaggc
     2941 cgaagcggct gatccagcgt gcgaccagct cgccgtcgcg ggagcggctc gcgagcgcgt
     3001 ggctgcctcg gcggccgtag aagaggggaa gaagcagcat gcgcccgtgc cacaccgcgt
     3061 agatgacggg ggcgcgggcg gtccagcacg gcatcaccgc ggcctcctcg cgccgcacgt
     3121 ggagcgtgag agcgagcgcg cgcagcgcgg aggcgccgag ggcaggcccg accgcgaggg
     3181 cgagcggtgc cgggatcttc atcttgcgtg tggggcggcc tgcggggcag ctcgcgcggt
     3241 ggcgagcacc gcgcgggcgg cgcgccgccc gactccgggc tcgccgagcc gcgcccgcag
     3301 ctccttgaga gccgcgcgct gggcggtggc cgccaccgga tcgtcgagga ggcgcgtcgc
     3361 ctcggccgcg aggcgctcgc cggtcacctg cgcctggatc agctcgggca ccaccaggcg
     3421 gcccgacacc aaattaggca gtccgatcca ccgcacgcgc gcgagcaggc gggcgatgac
     3481 ctcggtggtg cgggagacgc gatagcagag caccatcggc gtcccgagca gcgccgcctc
     3541 gagggtggcg gtgccggagg cgatgagcaa cgcgtcggcc gcggccatca cctcgtaggt
     3601 gcggccggcg acgatctcga tcgcgggccc ggggcctccg tggcgcaggt gggcctgcac
     3661 ctgctcgaga ggcacggtgg gcgccaggcc cagcacgaag cgtcgccgac cgtcggcggc
     3721 ggcgagccgc cgcgccgcct ccagcatggg gggcagcagc cgctcgactt cctcgcgccg
     3781 gctgcccggc agcagtccaa ccagcgagtg gccggggtcc gccgggaggc ggcggcgcgc
     3841 ctcgtcgtgg gtcaggtcga gcgggagcac gtcgagcagc gggtgtccca cgaactcgac
     3901 cggcacgccg gcccgctcgt agagcgcggg ctcgaaggga aagaccgcga gcacccggct
     3961 cacccgacgc gccatctggc ggacccggcc ctggcgccag gcccagagct ggggcgggat
     4021 gaagtagacc accggcaccc cggcccggcg ggcgcggcgc gcgagccgca ggttgaactc
     4081 cgggaagtcg atcaggacca gcgcgcgggg acgctccacg tggagccggg ccccgagcgc
     4141 gcggtaggcg cggtagaggg ccgggatgcg gccgaccgcc tcgctcgtgc cgaccgcggc
     4201 gtgctcggtc ggatcgacga cgacgtccat cccggccgcg cgcatgtgcc cgccgcccat
     4261 gccgacgagg cgcaccccgg gctccatctc gcgcagggcc cggcagagca tgccgccgtg
     4321 gagatcgccg gaggcctcgc cggccgagag catgaccggc gcgcggctcg tcgcgggctc
     4381 agcggccgcc atcgacgctc accagcgcga tccccgaccg atcggcgagc cgcacgacgg
     4441 gctcgcggtc gacgagcagg acgcgtccgg ccgggacggc cagcgcggtg gcgccgctct
     4501 cggccatcgc ctcgagggtg gcggtgccga tggtggggac gtcgaagcgg tagtcgtgcg
     4561 aggcggctac cgtcttgacc accaccgcgc ccggcccggc gaggcggcct ccgcgccgga
     4621 tcgtctcgtc ggttccctcg gccgcctcca ccgccaccgt cacccctcgc gcgcgcacca
     4681 cggtctgccc gatgccgtcg tcggccaggc gaccggcgag ccggaagccc tcgcggatct
     4741 cgtcccactc ctcgggcgac ggcgcgcggg cggagagcgt gccggcgggg agcagccacg
     4801 gggccaggaa ctcgcgctgg tcgaggacgt cgatgcccat gccggagagc gtggccacca
     4861 ccatctgggc gagtgctccg tcggagaggc cgccgcgggc gaggccgcgc ccggccgcgt
     4921 ccgcctggtc gtactgggag aaggcgctcg acttccagaa cttgccgacg aagacggcgg
     4981 cctcgacccg ctgggcggcc aggcccgcga gcaccgcctg gatgtcggtg atcgccgacg
     5041 ggatgacgtg gtcggcggag tcggccaggc cgggagcctc ctcgaaggcg aaggccacga
     5101 cgcgccagcc gtggcgccgg gcttccaggg cggcgcggcc gggaagcgtc ccggccccgg
     5161 ccatcaggcc gagcgaacgc tccacggaag atcctccggc caggcggtct cgccccggcc
     5221 ctcgcggatc atccgctcga tctccagcgc gacggccagc gatcgcaggt cgtccgcctc
     5281 gggcagctcc accggcccgc tgctgtcggc ggcccgcacc gccgagacca ggtggcggat
     5341 ctcgagcttc aaggggttgt ccttgtgcac gaggaggtgc tccacgaagg acgcctggcg
     5401 gtagcggatg gactcgcggt tgagggtgta ctcctgggcc gcgcgccggt ggatctggat
     5461 gtcctggtgc gagtagtcga gcaggatgta ggcgtcgggc tgggtgatgg cgagggtgcg
     5521 gatcttctcc tcggtggccc ggctcgcggt gatggtggcc atcgcgcccg agtcgaacac
     5581 gatctgcacg ttggccacgt cggtcgcgcc cgagtgcacc gacgagccga ccgcggtgat
     5641 cttgcgcggc tccccgtcga cgagcccgag cacgatgtcg atgtcgtgga tcatgagatc
     5701 catcaccacc gaatcgtcct gcacgcgcgg cacgaacggg ccgagccgcc gggactcgat
     5761 gaggatcggc cgctccacga tcttgcgcag ctcctggacc gcgccgttga accgctcgac
     5821 gtggccgacg tggagcaccc ggcgctgctg gcgggcgagg cggaagagct ccttggcctg
     5881 ctccagggtc gaggtcatcg gcttctcgac cagcacgtgg gcgccggctt ccagcaggtc
     5941 gcgcgccacc gcgaagtgct gctcggtggg gaccgccacg gtggcgaagt cgacgaggcc
     6001 cgcgagctcg cggtgatcgc ggaaggcccg cgccccgtag ggcgcggcca cctgctgcgc
     6061 gcgcgcgaag tcggtgtcca ccaccccgac cagctcgacg ccccagatct cggcgagggc
     6121 caggatgtgg tactggccca tgtggccggc ccccaccacc gcgccccgga tcttgggctc
     6181 ggtcccgttc gtcacgagat ctcctcctcg accgcgtcga ggcgcgcgcg accggtccgg
     6241 tccgcccccg ggaccacccc gcgcttggag gcctccacga actccaccag gcggtcgacc
     6301 agcgggtggc cgcccagctc ctgcttcacg tgcttgatcg cggcgccggg ggccatgccg
     6361 gaccggtaga ggagccggaa cgcggcccgc accttcaggc gcgacgcgga atcgatgccg
     6421 ccgcggcgca tcccgaccac gttcaccgag cgggccaccg cggggatgcc atccgccatg
     6481 atgaagggcg ggacgtcctg ggtgaccttc gagcagccgc cgatgtaggc gtaggtaccg
     6541 atgcggctga acgggtggat gccggtgagc ccgccgatgg tcacccggtc ctcgaccgtg
     6601 atatgcccgg tcaggcccgc gtagttgatc atgatgatgt ggtcgccgag cacgcagtcg
     6661 tgcgcgatgt ggctcgaggc catcaccagg cagtggcggc cgacggtggt gtcgtggccc
     6721 tcgtgggtgg cgcgatggat ggtcacgtac tcgcggacca ccgtgtcgtc gcccaggctc
     6781 acccccaccg gggtgccctc gcggaacttg aggtcctggg gcacgccccc gatgatcgcc
     6841 ccgtgcccga cccggcagcg ggctccgacc cggacccggc cctcgagcac cgcgtgggcg
     6901 ccgatctccg ccccggccgc caccgtgacc tcggggccga tcaccgagta cggcccgacg
     6961 cgcacgtcgg cggccagctc cgcgccgcgg tcgacgacgg cggtgggatg gatcacgacc
     7021 gccgctcctc cagctcgaga ggatggatca cgaccgccgc tcctccagct cccgcagccg
     7081 cttctcgagg gcacggaccc gcttgatcag ctcggggagc tggctctccc ccgcccagat
     7141 gcgcttctgc tccgaggtcg gccggctcgg gatgccgctc cacacttccc cggccgggac
     7201 gtcgttgggc acgccggact tggcggtgag gatggcgccc gccccgatcg tcacgtggtc
     7261 ggccacgcca acctgcccgg ccagcatggc ccggttgccc acccgcgagg agcccgcgat
     7321 cccgacctgg gagaccagga tcacgtcttc gcccacgtcg cagttgtgcc cgatctgcac
     7381 caggttgtcg atcttgctgc cgcggcgcac gatcgtctcg ccgagggtcg cgcggtcgat
     7441 cgcggtgttg gccccgatct cgacgtcgtc ctcgatgcgc aggccgccca cctgcgggat
     7501 cttgcggtgg gcctgcccgt cgaacgcata gccgaagccg tcggcgccca gcaccgctcc
     7561 cgagtggacg atgacccggt tgccgatacg cacgtcctcg cggaccacca cgtgggggta
     7621 caggcggacg tcctcgccga gcaccgcgcc gcgccccacg aacgagagcg cccccacgcg
     7681 gctgcgcggc ccgatcaccg cctccgcctc caccaccgcg agcgggccga tggacgccga
     7741 gcggtcgacg cgggcgtccg cggccacgca ggcggtcggg tgcactccgg gctcggcgcc
     7801 cggctcggga tggaagagcc cgagcagcgc gatcagcgcc tgctgcggat gatccacctg
     7861 gagcagcgcg ccggggaggc cggtgacgtc gcgcgccacc agcagggcgc cggcccggct
     7921 cgcctgggcc tgcttgagat atttccgatc ggtgaggaag gagacctgat cggggcccgc
     7981 gacgtcgagc ggcgccaccc cgcgcaccac gcgcccggag tcaccgtgga gcgacgcccc
     8041 gagcgcgtcg gcgagccggc cgagcgtgaa cgagacgccg tcgctcactt acttcgtggc
     8101 cttcttggtc tggtcgtcgt aggccctgag gacgtcgtcg gtcaggtccg cgtcggtgga
     8161 ggcgtagagc acgccggcgc gctggcgctc gacaaccacc gcgaagccct tctcttttcc
     8221 gaggcgctgg atgaggccgg ccacgtcctg gagcaccttc tggaggaggg cgtcctcctt
     8281 cttctggagc tgggcctgga gatcgtccac caggcgccgg acgtcccgga ccttgcgctc
     8341 gagcgcgtcc tgcttctcgc gccgggcgtc cgcggagagg agctggccct tcttctcgag
     8401 ctcgtcccgc atcttctcca gctcgacctt ttgcccgtcg agctggcgct gcatggccgt
     8461 cttgtccttc tcgagctgct cgcgcgccgc cgcgcccgcg accgagcggg cgaggatccg
     8521 ctggatatcc acgatcgcca cccgcccgtt cgacgcgggg gagggggccg gggtctgcgc
     8581 ctgggcgtgg gcccacatcg cgcccccgac gctcgtcagg agcgtcaagg tcgccgcggt
     8641 ccaccaccct gccatccatt gcctcatgtg ctacctcctc aactagaacg aggagccggc
     8701 ggagaagttg aagttgccga agctctcgcc cttcttctgg tcgagtttga tcccgtagtc
     8761 gatgcggatg gggccgaagg gcgagttcca ccggaggccc gcgcccaccc catgccgcag
     8821 gtcgctgagg tcgatcttgg tccccgccga gatgtcaggc ccccacacct gccctacatc
     8881 gtagaagacg gcggccttga tcccgaagaa aagagggatc tgatactcga cggtacccag
     8941 ggcctcgctg ttgccgccga tccgggtgcc ggtgttgtcc ttgggggaga cctggaggga
     9001 tttgaactgc cgcagggagt tcgagccacc caggtagaag cgctcgaaga gcggcaccgg
     9061 gtccttgctc cagccgaagg agtagcccgc catcaaccgg aagctcagga cgtgatcgag
     9121 ccagggcatg ggctggaagt aggtggtaga gaagacgctg cgggcgaact tctccccgaa
     9181 gccgacgccc gcgaagtcga ccccgatcga gacggtgctg ccccgggtcg gatcgtagac
     9241 gttgtcgcgg gtgtcccggg agagattcag cccgaccaga gaggtgaggt gggtaccctc
     9301 ttgggagatg agttccgggc tgccgagcgg gttgacgtcg ctgatcttgt cctggctgac
     9361 tcgatagagc gcgttccagc gcgagtactc tccgatgggg tgcccgagtc tgatgtcgcc
     9421 tccgagcgag ttgacggtgt agtcgggcag gagtcgtcgg gtgttgaaga tgtcgaagcc
     9481 ggcggccagt ggctgatcga agagccaggg ctcggtgaag ccgatggtcc cggtctggat
     9541 gttctggccg ccccgtaggc ggaggaacac ctcccagccc ttgccgagga agttccgctg
     9601 cgacaggtcg agggtgccga ggacgccgtc ctgggagctg tagccgccgc cgatcg
//
LOCUS       DATMCV010000295         2743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_750195, whole genome shotgun sequence.
ACCESSION   DATMCV010000295 DATMCV010000000
VERSION     DATMCV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750195"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /gene="tcuB"
                     /locus_tag="VNU03_06800"
     CDS             <1..864
                     /gene="tcuB"
                     /locus_tag="VNU03_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HWP74203.1"
                     /translation="RAETWEEYAWPGFLAGLFRRNGTVVALATALGIALLLLLTGLLR
                     DPAVLYGVHTAPGAFYLVIPSWAMISVAAATFSFSIVALIAGAGRYWKGTASGPFPGA
                     RAWWRALVDVATLRNLGGGGDGCNEQDEGFSQARRWLHHALFYGFALCFASTTVAAFY
                     EHGLGRLSPFPFASLPVLLGTLGGLGMTVGTAGMLWFKIAADPEPGAPRLLGTDFAFI
                     LLILLTAVTGLVLLALRATGAMGTLLAVHLGCVLALFVVMPYSKFVHGIYRGAALLRN
                     AVEQAAAPRDD"
     gene            877..2091
                     /locus_tag="VNU03_06805"
     CDS             877..2091
                     /locus_tag="VNU03_06805"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP74204.1"
                     /translation="MRLLDGIRVIDAASYIAGPVATTAMADFGADVIKIEPPSGDTYR
                     LRNAGYPPSPHNFPWIVDNRNKRSVVLDLRSEDGRGVLHRLVRGVDVFVTNAPLDGRG
                     RLGVRWEDLSPLNPRLIYGSITAYGEAGAEASRPGFDSTALWARTGLMDLVRPSPEAP
                     PSRSLPGMGDHPTGMSLFGAIMAALYQRERTGRGAMVSTSLLANGLWLNAIAVQGILC
                     GARTVVRPPREDALDALGNLYRCRDGRWFLIALTPDGRRWQDFTAAIGRPELFADPRF
                     ATPTARRGNTRALIAILDAVFVERDWAEWRRVLEASGIAFGVVGTLDDIAHDPQMRDS
                     GALVPLDDPRAGAALTVSSPLWIEGQEKVAATMAPGLGEHTLEVLREAGLDEADLARL
                     QQAGVIVQGKPA"
     gene            complement(2096..>2743)
                     /locus_tag="VNU03_06810"
     CDS             complement(2096..>2743)
                     /locus_tag="VNU03_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HWP74205.1"
                     /translation="TIPIVAMDLDGDALAPALAAGRPPADNVTGVALDVADLSVRQLE
                     LLRELLPSLAAVTILGDPEVHIAHFQAMQRAGSARSVTIRDLEIRSAQEFGDAFAMPP
                     GRAGGALVVLSSPRLFAFRRDAAEIARERGMPALFLYPAYADAGGLAAYGPDLPEVFR
                     RCGEYVGRILAGARPAELPLARPERFRLVVNERTARALGVTIPESVRRRADQVIE"
BASE COUNT          334 a         1059 c          975 g          375 t
ORIGIN      
        1 cgcgccgaga cctgggagga gtacgcgtgg cccgggttcc tcgccgggct gttccgccgg
       61 aacggcacgg tggtggccct cgccaccgcg ctcggcatcg cgctgctgct cctcctcacc
      121 gggctcctgc gcgatccggc ggtgctctac ggcgtgcaca ccgcgccggg cgcgttctat
      181 ctggtgatcc cgtcgtgggc catgatctcc gtggcggcgg ccaccttctc gttctcgatc
      241 gtcgccctga tcgcgggcgc gggccgctac tggaagggca ccgcgtcggg cccgttcccg
      301 ggcgcgcgcg cgtggtggcg cgcgctcgtc gacgtggcca ccctgcgcaa cctgggaggc
      361 ggcggcgacg ggtgcaacga gcaggacgag ggcttctccc aggcgcgccg ctggctgcac
      421 cacgccctct tctacggctt cgccctctgc ttcgcctcca ccaccgtcgc cgcgttctac
      481 gagcacgggc tggggcggct ctccccgttt cccttcgcga gcctgccggt gctgctcggc
      541 acgctgggcg ggctcggcat gaccgtcggc accgcgggca tgctctggtt caagatcgcg
      601 gcggacccgg agcccggcgc gccgcgcctg ctgggcaccg acttcgcgtt catcctcctc
      661 atcctgctga ccgcggtgac cgggctcgtg ctcctcgccc tgcgcgcgac gggcgccatg
      721 ggcaccctgc tcgcggtgca cctgggctgc gtgctggccc tcttcgtggt gatgccctac
      781 agcaagttcg tgcacggcat ctaccgcggg gccgcgctcc tgcgcaacgc ggtcgagcag
      841 gcggccgccc cgcgcgatga ctgaggcgag gcgccggtga ggctgctgga cggcatccgg
      901 gtgatcgacg ccgcgagcta catcgcgggg ccggtggcca ccaccgccat ggccgacttc
      961 ggcgccgacg tgatcaagat cgagccgccg agcggcgaca cctaccgcct gcgcaacgcg
     1021 ggctacccgc ccagccccca caacttcccc tggatcgtcg acaaccggaa caagcgcagc
     1081 gtggtcctcg acctccgctc cgaggacgga cgcggggtgc tgcaccgcct ggtgcggggc
     1141 gtcgacgtgt tcgtcaccaa cgctccgctc gacggacggg gccgcctcgg cgtgcgctgg
     1201 gaggacctgt ctccgctcaa cccccggctg atctacggct cgatcaccgc ctacggggag
     1261 gccggcgcgg aggcgagccg gcccggcttc gacagcaccg cgctctgggc ccgcaccggg
     1321 ctgatggacc tggtccgccc gtcgccggag gcgccgccct cgcgctcgct gcccggcatg
     1381 ggcgaccatc cgaccggcat gtcgctcttc ggcgcgatca tggccgccct ctaccagcgc
     1441 gagcgcaccg gccgcggagc gatggtctcc acgtcgctcc tcgccaacgg gctctggctg
     1501 aacgccatcg cggtccaggg catcctgtgc ggcgcgcgca ccgtggtgcg cccgccgcgc
     1561 gaggacgcgc tcgacgccct cggcaacctc tatcgctgcc gcgacggccg gtggttcctc
     1621 atcgccctca ccccggacgg ccgccggtgg caggacttca cggcagcgat cggccggccg
     1681 gagctcttcg ccgacccgcg cttcgccacc ccgaccgccc gccgcggcaa cacccgcgcc
     1741 ctcatcgcga tcctcgacgc ggtcttcgtc gagcgtgact gggcggagtg gcgccgggta
     1801 ctcgaggcga gcggcatcgc cttcggcgtg gtgggcacgc tcgacgacat cgcccacgac
     1861 ccgcagatgc gcgatagcgg cgcgctcgtc ccgctcgacg atccccgcgc cggcgccgcg
     1921 ctcaccgtca gcagcccgct ctggatcgag gggcaggaga aggtggccgc caccatggct
     1981 ccgggcctgg gcgagcacac cctcgaggtc ctccgcgagg cgggcctcga cgaggcggat
     2041 ctcgcgcgcc tgcagcaggc cggcgtgatc gtccagggca agcccgcctg agcggtcact
     2101 cgatcacctg gtcggcgcgc cgccgcaccg attcgggaat ggtcaccccg agcgcccggg
     2161 cggtccgctc gttgacgacc agccggaagc gctccggccg cgcgagcggc agctcggcgg
     2221 gccgggcgcc ggccaggatc cggcccacgt actccccgca gcgccggaac acctccggaa
     2281 gatcgggccc gtaggcggcg agaccgccgg cgtccgcgta ggccgggtag aggaagagcg
     2341 cgggcatgcc ccgctcgcgc gcgatctcgg ccgcgtcgcg ccggaacgcg aagagccgcg
     2401 gcgaggagag cacgacgagg gcgccgcccg cccggcccgg cggcatcgcg aaggcgtcgc
     2461 cgaactcctg ggcactgcgg atctcgaggt cccggatcgt caccgagcgc gcgctcccgg
     2521 cccgctgcat ggcctggaag tgggcgatgt ggacctcggg atcgcccagg atcgtcaccg
     2581 cggcgaggct cggcagcagc tccctgagca gctccagttg ccggacgctc aggtcggcca
     2641 cgtcgagcgc cacgccggtc acgttgtccg ccggcggccg gcccgccgcg agagccgggg
     2701 cgagggcatc gccgtccagg tccatggcca cgatggggat ggt
//
LOCUS       DATMCV010000296        12033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_616273, whole genome shotgun sequence.
ACCESSION   DATMCV010000296 DATMCV010000000
VERSION     DATMCV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12033
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616273"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..2246)
                     /locus_tag="VNU03_06815"
     CDS             complement(48..2246)
                     /locus_tag="VNU03_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP74206.1"
                     /translation="MDAVREFVERDVRPTASALEHADRYPRDLVERMARMGLFGALVP
                     AQYGGLGLDVSTYARIIEELCRGFMSLAGVINSHTMAALIVYHHGTEEQRLRLLPRFA
                     SGQARGGLCLTEPHAGSDVQAIRTVARRAGDRYLLSGSKMFVTNGREGNTFALLALTE
                     PDARPRHRGMSCFIVEKGAPGLEVVKSIAKLGYKGVDTAELVFDGFPCPAANLVGGVE
                     GRGFKHVMSGLETGRINIAARAVGVAQAGLDEAVRVARALPEPPAALADIATRVESAR
                     LVTYWAAAMKDRGDRCDLEAGMAKLHASEAAQEVAVETMRLEGARGQLASGLAERLYR
                     DAPLMIIGEGTNEIQRLIICRNLLERYGERPGALTSRDDLPDEQRQLVLAVRQAVEKD
                     VAPVIHDYDAGRRDPEPLVARLAELGLFGALAEPEHGGLGLSLATAAMIVEEVARGSA
                     TLGGWLGAQLAASLAIARHSPRDVRERLLPPMTRGERWTAPIIGPSIGARQAGKDWVL
                     TGSAPVVANAGRAKLFLVNTALEDGRSRCFAVEAGRAGLTVGLASDTVGLRGLGAADV
                     VLVGVRLGTTAILREGAETLLEAVSSVGTAATAVGLAQAAFEAALRYSQQRSTFGQPI
                     CQHQAIQLKLADMATRITVARLLTARAAARLDVDSRDDAGATMARLAAGETAYAVSLE
                     AMRIHGGYGYTSEFPVERYYRDAAALLLGPREPAAERRRLAARWLASQLP"
     gene            2404..3096
                     /locus_tag="VNU03_06820"
     CDS             2404..3096
                     /locus_tag="VNU03_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWP74207.1"
                     /translation="MNFTVASYNIHKCAGLDGRVDLDRTADVIQEIDADLVGLQEVFR
                     PQALSLAERLGVQVAMGPTRERDGLPYGNAILTRLAIRGSRIFDLSQPDREPRGGIRL
                     DLEMAEGPLHLFNVHFGLQIRERAEQVRRLVREHILHDELTGPRVVVGDLNEWFWFPG
                     AVGRTLRRELHGPRIRRTHPAPLPLFPLDRIYWDRDLAADGFHVHRSRLARVASDHLP
                     VVARLRMAPARG"
     gene            3122..4465
                     /gene="mgtE"
                     /locus_tag="VNU03_06825"
     CDS             3122..4465
                     /gene="mgtE"
                     /locus_tag="VNU03_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWP74208.1"
                     /translation="MADTERLLHAAQQLGRGEADVADVELVRDAHPADLAHVLQHLAP
                     EERHGLFRSFGRDRAGDVLSEMDDHTLLELIRGLDDVELSRILEQMPAEHAADVVDEL
                     PTEQAEKILDLMEEQKSEEVQGILEYPEQSAGRLMSPDFVARPEDATVEDAIQYIRRS
                     IAQERPFELYVVDDHQHLVGRVPLRRLLIAAPRTTLATLLDDELVSVRPEMDREDVAR
                     LVAKYDLVSLPVVDEQNRLLGTISVDDVIDIMQEEASEDIFRIAGSDAAELERRTPYQ
                     VALLRLPWVLSTLLIELLAGLIIHFFDRTLGQVILLASFMPVIQAISGNTGLQSVTMV
                     VRGLATGQVQLSRWWEPLRRQAQTSSILGAVCALVVGVVGWLWHSPAFALVVSVSMFI
                     SVNLSGTAGTVIPMLSKRLGFDPALTAGPFETAFQDVLGVTIFLSLATLLLHWLT"
     gene            4643..5053
                     /locus_tag="VNU03_06830"
     CDS             4643..5053
                     /locus_tag="VNU03_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP74209.1"
                     /translation="MTYPTQALAESGPSRLPSRALVVDDSMVVADVVTALLRRTGWTV
                     DVANTVDAALEHVRGVPYDLVVCDVCMPDGGGPAVYYAATTRRPDLASRFLFITGNMD
                     DPEPWRFLAKIRAHVLEKPFTGRALRHALIKVIA"
     gene            5158..5622
                     /locus_tag="VNU03_06835"
     CDS             5158..5622
                     /locus_tag="VNU03_06835"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74210.1"
                     /translation="MKVLQDPALDQTQRRAEIRAIALEAFDVTEAARRTLGPHWPKRT
                     PAERQEFIGLFQGLLERGYLSRLGEYGGESVQYVGERIEGEYATVRALIVTQKGTQVP
                     VEARVLRQGDRWRMYDVLIENVSLIASYRSQFDRVIRTSSYEELVRRLKSRE"
     gene            complement(5606..6847)
                     /locus_tag="VNU03_06840"
     CDS             complement(5606..6847)
                     /locus_tag="VNU03_06840"
                     /inference="COORDINATES: protein motif:HMM:NF006769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HWP74211.1"
                     /translation="MRINRARLAESMETLGKIGETARGGLHRVALTDDDRRGRDLLVR
                     WMKEAGLAVTVDQMGNIFGLRAGTEARPPVFMGSHADSVPSGGKYDGQLGVLCALETL
                     RSLNDRQARTRHPVGMIVFTNEEGARFQPAMIASGVMAGKIPLEDAYNTRDRDGLRLV
                     DELERIGYLGSEPCVPRPMRAYLELHIEQGPLLEEEGLPLGVVEGIVAIAWSRITLHG
                     VQDHAGPTPMRLRHDALVAAAEIVTGVRGIARRIAGDMVATVGRLDLTPNIPNAIPGR
                     VSLSVDFRDPQEANLDRALAMLDHVVHVAARQEGVRAEVEHYWRVPRTPFDPEVVEVV
                     EAAATSLGCGHRRILSGAGHDAQYMAAICPTGMIFVPSRGGRSHCEEEFTPLDDIEHG
                     ANTLLLACARLAGAESATRAT"
     gene            complement(6859..7563)
                     /locus_tag="VNU03_06845"
     CDS             complement(6859..7563)
                     /locus_tag="VNU03_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74212.1"
                     /translation="MLTLEGVDAFYGDLQALHGVSLEVRAGELMALVGANAAGKTTTL
                     RVISGTLPARGGRVLFDGQDLASMPAHLRVAQGIVQVPEGRRLFPFMTVHENLLLGAH
                     APQARAQQEASLDYVLRLFPVLKDRATQLAGSLSGGEQQMCAIGRALMARPRLLMLDE
                     PTLGIAPVLVQRIFETVAAINRDGVTVLLVEQNVRRALALAHRAVALENGRVVLSGPA
                     RELLADDRLKKAYLGM"
     gene            complement(7550..8275)
                     /locus_tag="VNU03_06850"
     CDS             complement(7550..8275)
                     /locus_tag="VNU03_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74213.1"
                     /translation="MLELDAVTKRFGGLTAVKDVSLRAAPGEILGIIGPNGAGKTTLF
                     NVISGFYAPNTGTVRLDGRDISGLAPHHVCALGLTRTFQLVKPFGNLSVLDNVMIGAL
                     TRVHAVSAAREAARRVIATCRLEAFAESRARSLPIGLRKRLEVARALATQPRLLLLDE
                     VMSGLNPTELVEMIEILRRLHGDGLGLIVIEHIMAAMMRLAQRIVVLHHGEKIAEGRP
                     EEISRDPRVVDAYLGEEFVFADA"
     gene            complement(8262..9233)
                     /locus_tag="VNU03_06855"
     CDS             complement(8262..9233)
                     /locus_tag="VNU03_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74214.1"
                     /translation="MKRDVPALVLFGLAALAPLVTRDGFLLDSLILILLWGAVSGAWN
                     VAGGYAGQVSLGHSAFFGLGAYSAALLATRWNQSPWLGLLVGALLATVAGLVIGYLAN
                     RLKGPYFGLATIAFSQVLLILASRWRAVTSGSEGIPVPFRPGFATLGFQSKIPWVYLV
                     LTLAVGIYLVARYLETSRPGYQLAGVREDEDAAEALGIPCRRLKVAAVAASAALTSVC
                     GGLWAQYVGFVDPFYVFSVDLSVRFALNSIIGGLGTALGPFVGSLLITSLETYLRATF
                     SDFKAGLIGIYLIIYGCLLIVVVRFVPQGLVGWIGERLRAMGRYARA"
     gene            complement(9238..10119)
                     /locus_tag="VNU03_06860"
     CDS             complement(9238..10119)
                     /locus_tag="VNU03_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74215.1"
                     /translation="MGAVPQLIVSTVLLGGIYALIAVGLTLIFGIMRVVNFAHGEFLM
                     LGMYLAFWSFTLWGLDPYVILLVAVPLFFAIGLLTYALVMRGVVKASHNVQIFTTVGL
                     STALQNAALVAWTGDFRFVRPWHSGVVIRIGEAAFNLSQVVAFLVAVLFTAALVAFMK
                     WTRAGRIMRATAQDVEAATLMGIDTDRVYRLAWSIGLVCVGIAGVLMSPLYSVYPTAG
                     LQFVLLAYVVVVLGGLGDMIGALLGSLIVAGVEVLGSYYLGSAWKEVIYFVLFIAVLV
                     FRPAGLFGQRGSETLGA"
     gene            complement(10135..11421)
                     /locus_tag="VNU03_06865"
     CDS             complement(10135..11421)
                     /locus_tag="VNU03_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74216.1"
                     /translation="MSKRRGRLTALVLLIAVTLVSAALIRGTEGQALQGEYKIGVLEP
                     LTGALAFEGKRHLEGYEIMRDMINERGGVMGKKLVFVVGDGVDPTAAASEANRLITRE
                     GVKIITGTYSSTLCGAASEAASRQNVIYWESSCVDPRFSKRGLKNVFRTEIDGTGFGW
                     YNVEFIAKHLAPRFNLKPNQIKIAFLSEDSSYGQGVTEAARERAKKDFGMQEVAMEYY
                     AFASTNDFSPIVAKLKQVNPDVVHHIARGNDAVLFWRQSREQNFMFKALVHAGATGYG
                     SPDFGKALGAEGNGPFVLAEPSTGLRLEAFRPEAQKLERDFTEAVKKRAGTEPYASHR
                     LAGAGLWLLKLVLDQAKTDELEKFRSAVMSMDLPVGSMLNGWGVKFAENGQNANERVQ
                     HYMLQWQNGQMVTVWPEEFSTLRAKWMPLPAWDQRK"
     gene            complement(11498..>12033)
                     /locus_tag="VNU03_06870"
     CDS             complement(11498..>12033)
                     /locus_tag="VNU03_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWP74217.1"
                     /translation="EHFTYRKNVTRYDSTTDDFLGLGAGVCQDFTHLMLGLLRLRQIP
                     CRYVSGYLHVSPAEGEVAQSHAWIEFHSPAAGWVPFDPTHNRPVDDHYVVVGHGRHYD
                     DVPPNKGIFRGNADEILKTEVRTQASTPKDISALHEEMEQIDVPVYQEVPERRGDRLR
                     QAAEEIAAQEQQQAEEQ"
BASE COUNT         1738 a         4263 c         4320 g         1712 t
ORIGIN      
        1 ggtgaactcc tggcccgcgg cgaactcctc gtagtaccgc ccgtagctca tgggagctgg
       61 ctggcgagcc accgggccgc gagccgccgc cgctcggcgg cgggctcgcg cgggccgagc
      121 agcagcgcgg ccgcgtcgcg atagtagcgc tcgaccggga actcgctggt gtagccgtac
      181 ccgccgtgga tgcgcatcgc ctcgaggctc accgcgtagg cggtctcgcc cgccgcgagc
      241 cgggccatgg tggcgcccgc gtcgtcgcgc gagtccacgt cgaggcgcgc ggccgcgcgc
      301 gcggtgagca gtcgcgcgac ggtgatgcgg gtggccatgt cggccagctt gagctggatg
      361 gcctggtgct ggcagatcgg ctggccgaac gtggagcgct gctgcgagta gcggagcgcg
      421 gcctcgaagg cggcctgggc cagcccgacc gcggtggcgg cggtgcccac gctgctcacc
      481 gcctcgagca gggtctcggc gccctcgcgg aggatggcgg tggtgcccag gcggacgccg
      541 accagcacca cgtccgccgc gcccaggccg cgcaggccca cggtgtcgga ggcgaggccc
      601 acggtgaggc cggcccgtcc cgcttccacc gcgaagcagc ggctccggcc gtcctcgagc
      661 gcggtattca cgaggaagag cttggcccgg cccgcgttgg ccaccaccgg ggccgatccg
      721 gtcaggaccc agtccttgcc ggcctggcgc gccccgatcg acgggccgat gatcggcgcg
      781 gtccagcgct cgccgcgggt catcggcgga agcagccgct cgcggacgtc gcggggcgag
      841 tggcgcgcga tggcgaggct ggccgcgagc tgggcgccga gccagccgcc cagggtcgcc
      901 gagccgcggg ccacctcctc cacgatcatg gccgcggtgg cgagcgagag accgagaccg
      961 ccgtgctcgg gctcggcgag cgcgccgaag aggccgagct ccgcgagccg cgcgacgagc
     1021 ggctcggggt cccggcgccc cgcgtcgtag tcgtggatca ccggcgccac gtccttctcc
     1081 accgcctgcc gcaccgccag gaccagctgc cgctgctcgt cggggagatc gtcccggctg
     1141 gtgagggccc cggggcgctc gccgtaccgc tccagcaggt tccggcagat gatgagccgc
     1201 tggatctcgt tggtgccctc gccgatgatc atgagcggag cgtcgcgata gagtcgctcg
     1261 gccagcccgg aggcgagctg gccgcgcgct ccctcgagcc gcatggtctc gacggccact
     1321 tcctgggcgg cctccgaggc gtgcagcttg gccatgccgg cctccagatc gcagcgatcg
     1381 ccccggtcct tcatcgccgc ggcccagtag gtgaccagcc gcgcggattc cacgcgcgtg
     1441 gcgatgtcgg ccagggccgc gggcggctcg ggcagggcgc gcgccacccg caccgcctcg
     1501 tcgaggccgg cctgggccac cccgaccgcc cgggccgcga tgttgatccg tccggtctcg
     1561 aggcccgaca tcacgtgctt gaacccgcgg ccctcgaccc cgcccaccag gttggccgcg
     1621 gggcagggga agccgtcgaa gaccagctcc gcggtgtcca cccccttgta gcccagcttg
     1681 gcgatggact tgacgacctc gaggccgggg gcgcccttct ccacgatgaa gcaggacatg
     1741 ccgcggtgac ggggccgcgc gtcgggctcg gtgagcgcga gcagcgcgaa ggtattaccc
     1801 tcccggccgt tggtcacgaa catcttggag ccgctgagca ggtagcggtc gccggcccgg
     1861 cgggccaccg tccggatcgc ctgcacgtcg gagccggcgt ggggctcggt gaggcagagc
     1921 ccgccccggg cctggccgct ggcgaagcgg ggcaggaggc ggaggcgctg ctcctcggtg
     1981 ccgtggtggt agacgatcag cgcggccatc gtgtggctgt tgatgacccc ggccagggac
     2041 atgaagcccc ggcacagctc ctcgatgatg cgggcgtagg tgctgacgtc gaggccgagg
     2101 ccaccgtact gggcggggac cagagccccg aagagcccca tccgcgccat gcgctcgacc
     2161 agatcccggg ggtaccggtc cgcgtgctcc agcgccgacg cggtgggccg cacgtcccgc
     2221 tcgacgaact cgcggacggc gtccacgagg gagggaagac ggcttgaacc tggactcacg
     2281 ggggccaacc tcgcgatgat cgctctcttt gctgcgtcga gactagcaaa gcccgcgagg
     2341 ccattgcaag gctcggcccc tcgtttcttg gacgcgggtc gccgggcccg gtatgctggg
     2401 ggcatgaact tcaccgtggc cagctacaac atccacaagt gcgcgggcct ggacggccgc
     2461 gtggatctcg accgcaccgc cgacgtgatc caggagatcg acgccgacct ggtcggcttg
     2521 caggaggtct tccggccgca ggccctcagc ctggccgagc gtctcggcgt ccaggtggcg
     2581 atggggccga cccgggagcg cgacgggctg ccctacggca acgcgatcct cacgcggctc
     2641 gccatccggg gcagccgcat cttcgatctc tcgcagccgg accgtgagcc gcgcggcggc
     2701 atccggctgg acctcgagat ggcggagggg ccgctccacc tcttcaatgt gcacttcggc
     2761 ctccagatcc gcgagcgggc cgagcaggtg cggcggctgg tgcgcgagca catcctccac
     2821 gacgagctga ccggcccgcg cgtggtggtg ggcgatctca acgagtggtt ctggtttccg
     2881 ggcgcggtcg gccggaccct gcggcgggag ctgcacgggc cgcgcatccg gcggacgcat
     2941 ccggctccgc tcccgctgtt cccgctcgat cgcatctact gggatcgaga tcttgccgcc
     3001 gacggcttcc acgttcaccg gagccgtctt gcccgcgtgg cctcggacca cctcccggtg
     3061 gtcgcccggc tgcggatggc gcccgcgcgc ggctgatccg ccgcgcgctg ccggtcgggt
     3121 cgtggcggac accgagcggc tcctgcacgc ggcccagcag ctgggccgcg gcgaggccga
     3181 cgtggcggac gtcgagctgg tgcgggacgc ccacccggcg gatctcgccc acgtcctcca
     3241 gcacctggcc ccggaggagc gccacgggct cttccgctcc ttcggacgcg accgggccgg
     3301 cgacgtcctg tccgagatgg acgaccacac cctgctcgag ctgatccgcg gcctcgacga
     3361 cgtcgagctc tcgcgcatcc tcgagcagat gccggccgag cacgccgccg acgtggtgga
     3421 cgagctgccc accgagcagg ccgagaagat cctcgacctc atggaggagc agaagtccga
     3481 ggaggtccag ggcatcctcg agtaccccga gcagtcggcg gggcggctca tgtccccgga
     3541 cttcgtggcc cgtcccgagg acgcgacggt ggaggacgcg atccagtaca tccgccgctc
     3601 gatcgcccag gagcgcccct tcgagctgta cgtggtcgac gaccaccagc acctggtcgg
     3661 gcgcgtcccg ctgcgccgcc tgctcatcgc ggcgccccgc accacgctgg ccacgctcct
     3721 cgacgacgag ctggtgagcg tgcggccgga gatggaccgc gaggacgtgg cccggctcgt
     3781 ggccaagtac gacctggtga gcctgccggt ggtggacgag cagaaccggc tcctcggcac
     3841 catctcggtc gacgacgtca tcgacatcat gcaggaggag gcctcggagg acatcttccg
     3901 catcgcgggc tccgacgcgg ccgagctcga gcggcgcacc ccgtaccagg tggccctgct
     3961 gcgcctgccc tgggtgctga gcacgctcct catcgagctg ctggccgggc tgatcatcca
     4021 cttcttcgac cgcacgctcg gtcaggtcat cctgctcgcg tcgttcatgc cggtgatcca
     4081 ggcgatctcc ggcaacaccg gtctgcagtc ggtgaccatg gtggtgcgcg gcctcgccac
     4141 cggccaggtc cagctctccc gctggtggga gccgctgcgg cggcaggcgc agacctcgag
     4201 catcctgggc gcggtgtgcg cgctcgtggt gggcgtggtc ggctggctct ggcactcgcc
     4261 cgcgttcgcc ctcgtggtct cggtgtcgat gttcatctcg gtcaacctct cgggcaccgc
     4321 ggggaccgtg atccccatgc tgtccaagcg attgggcttc gatccggcgc tgaccgcggg
     4381 ccccttcgag accgcgttcc aggacgtcct cggcgtgacc atcttcctct ccctggccac
     4441 gctgctcctg cactggctca catgaccacc gacgctttgt tgccgaccgg tgccacgctt
     4501 tgtatcggca gggggacgcg caatcttgcg gggtcaggag atcgatcaat tttctcgcta
     4561 tgttgggggc tctatcgcgc tggcaagcgc cttgctctct ccctctatcg ccatggacgt
     4621 gcggtcctcg gaaccggagc tggtgacgta cccgacgcag gcgctcgccg agtcgggacc
     4681 gtcccgtctg ccgtcgcggg ccctggtggt ggacgactcg atggtcgtgg ccgacgtggt
     4741 gaccgcgctg ctgcgccgca ccggctggac ggtggacgtg gcgaacacgg tcgacgcggc
     4801 gctcgagcac gtgcgcgggg tgccctacga tctggtggtc tgcgacgtgt gcatgcccga
     4861 tggtgggggg cccgcggtct actacgcggc cacgacccgg cgaccggacc tggcgagccg
     4921 cttcctgttc atcaccggga acatggacga cccggagccc tggcgcttcc tggccaagat
     4981 ccgcgcccac gtgctggaga agcccttcac cggccgggcg ctccgtcacg ccctgatcaa
     5041 ggtcatcgcc tgacccgccg gctcgcgccg gcgctgcttc tgctcctgac gctcgtcgcc
     5101 gcccccgcct gggcgggcgc tcccaccgag cagctccgcg cgctcaccga ccgcgtggtg
     5161 aaggtcctcc aggatcccgc gctcgaccag acccagcgcc gcgccgagat ccgcgccatc
     5221 gccctcgagg ccttcgacgt cacggaggcg gcgcgacgta ccctgggccc gcactggccg
     5281 aagcgcaccc cggccgagcg gcaggagttc atcggcctct tccagggcct gctcgagcgg
     5341 ggctatctct cccgcctcgg cgagtacggg ggcgagagcg tgcagtacgt gggcgagcgg
     5401 atcgagggcg agtacgcgac ggtgcgggcc ctcatcgtca cccagaaggg cacccaggtg
     5461 ccggtggagg cgcgggtgct gcgccagggc gaccgctggc gcatgtacga cgtgctgatc
     5521 gagaacgtga gcctgatcgc gagctaccgc tcccagttcg accgggtgat ccgcacctcc
     5581 tcctacgagg agctggtgcg ccgcctcaag tcgcgcgagt agccgactcg gcgcccgcca
     5641 ggcgcgcgca cgcgagcagg agcgtgttgg cgccgtgctc gatgtcgtcg agcggggtga
     5701 actcctcctc gcagtggctg cggccgccgc gggagggaac gaagatcatc ccggtggggc
     5761 agatggcggc catgtactgc gcgtcgtggc cggcgcccga gaggatgcgg cgatggccgc
     5821 agccgagcga ggtcgccgcg gcctcgacga cctccaccac ctccggatcg aagggcgtcc
     5881 gcggcacccg ccagtagtgc tcgacctccg cgcggacgcc ctcctgccgc gcggccacgt
     5941 gcaccacgtg gtccagcatc gcgagcgccc gatcgaggtt cgcctcctgc gggtcccgga
     6001 agtccaccga gaggctgacc cttcccggga tcgcgttggg gatgttgggg gtcaggtcga
     6061 ggcgccccac cgtggccacc atgtcccccg cgatcctccg ggcgatgccg cgcaccccgg
     6121 tcacgatctc ggccgcggcc accagcgcgt cgtggcgcag gcgcatcggc gtcggccccg
     6181 cgtggtcctg caccccgtgc agcgtgatcc gcgaccaggc gatggccacg atgccctcca
     6241 cgacgccgag ggggagccct tcctcttcca ggagcggacc ctgctcgatg tgcagctcca
     6301 ggtaggcccg catgggccgc ggcacgcacg gctcggagcc gagatacccg atgcgctcga
     6361 gctcgtcgac caggcgcagc ccgtcgcggt cgcgggtgtt gtaggcgtcc tcgagcggga
     6421 tcttgccggc catgaccccc gaggcgatca tcgcgggctg gaagcgcgcg ccctcctcgt
     6481 tggtgaacac gatcatcccg accgggtgac gggtacgcgc ctgccggtcg ttcaggctcc
     6541 ggagcgtctc gagcgcgcag agcacaccga gctggccgtc gtacttgccg ccgctcggca
     6601 ccgagtccgc atgcgagccc atgaacaccg gcggccgcgc ttccgtcccc gcccggaggc
     6661 cgaagatgtt gcccatctgg tcgaccgtga ccgcgagacc cgcctccttc atccagcgca
     6721 ccaggaggtc gcggccgcgc cggtcgtcgt cggtgagggc cacccggtgg aggccgcccc
     6781 gcgcggtctc gccgatcttg ccgagcgtct ccatgctctc cgccagccgg gcccgattga
     6841 tccgcatcgg ctccctcctc acatgccaag ataggccttc ttgagccggt cgtcggccag
     6901 gagctcgcgg gcgggcccgg acaggacgac gcgaccgttc tcgagggcca ccgcgcgatg
     6961 cgccagcgcg agcgcgcggc gcacgttctg ctccaccaag aggacggtga ccccgtcgcg
     7021 gttgatcgcg gccaccgtct cgaagatccg ctgcacgagc accggggcga tcccgagcgt
     7081 cggctcgtcc agcatgagca gccggggccg cgccatcagg gcccggccga tcgcgcacat
     7141 ctgctgctca ccgccggaga gcgagcccgc gagctgggtg gcgcggtcct tcagcaccgg
     7201 gaagagccgc agcacgtagt cgagcgaagc ctcctgctgc gcgcgcgcct ggggggcgtg
     7261 cgcgccgagc agcaggttct cgtgcacggt catgaacggg aagaggcggc ggccctccgg
     7321 gacctggacg atgccctggg ccactcgcag gtgggccggc atcgaggcga ggtcctggcc
     7381 atcgaacagc acacggccgc cgcgggccgg cagcgtgccc gagatcaccc gcagggtggt
     7441 ggtcttgccc gccgcgttgg cgccgaccag ggccatcagc tcgcccgcgc gcacctcgag
     7501 gctcaccccg tggagcgcct ggaggtcgcc gtagaacgcg tccacgccct caagcgtcag
     7561 caaacacgaa ctcctccccg agataggcgt cgaccacgcg ggggtcgcgg ctgatctcct
     7621 ccggccggcc ctccgcgatc ttctcgccgt ggtggagcac cacgatccgc tgggcgagcc
     7681 gcatcatggc ggccatgatg tgctcgatca cgatcaggcc gaggccgtct ccgtgcaggc
     7741 ggcgcaggat ctcgatcatc tcgaccagct cggtggggtt gagccccgac atgacctcgt
     7801 cgagcagcag gaggcggggc tgggtggcga gggcgcgggc cacctcgagc cgcttgcgca
     7861 ggccgatggg cagcgagcgg gcgcggctct ccgcgaaggc ctcgagccgg cacgtcgcga
     7921 tcacccggcg cgcggcctcc cgcgccgcgc tcaccgcgtg cacgcgggtg agcgccccga
     7981 tcatcacgtt gtcgagcacc gagaggttgc cgaagggttt cacgagctgg aaggtgcggg
     8041 tgagccccag cgcgcacacg tggtggggcg ccagcccgct gatgtcgcgc ccgtcgagcc
     8101 gcaccgtccc ggtgttgggg gcgtagaagc cggagatgac gttgaagagc gtggtcttgc
     8161 ccgccccgtt gggcccgatg atgccgagga tctccccggg cgccgcgcgc aggctgacgt
     8221 ccttcaccgc ggtgaggccg ccgaagcgct tggtgaccgc gtcaagctcg agcatagcgt
     8281 cccatcgcgc gcagccgctc cccgatccag ccgaccaggc cctggggcac gaagcgcacg
     8341 accacgatga gcaggcagcc gtagatgatc aggtagatgc cgatgagccc cgccttgaag
     8401 tcgctgaacg tcgcgcgcag gtaggtctcg agcgaggtga tgaggagcga gcccacgaag
     8461 gggccgagcg cggtgccgag cccgccgatg atactgttca gcgcgaagcg caccgagagg
     8521 tcgaccgaga agacgtagaa gggatccacg aagcccacgt actgggccca gagcccgccg
     8581 cacaccgagg tgagcgcggc gctcgccgcg accgccgcga ccttgagacg gcggcacggg
     8641 atgccgagcg cctccgccgc gtcctcgtcc tcgcgcaccc cggcgagctg atagccgggc
     8701 cgcgaggtct cgaggtagcg cgcgacgagg tagatgccga cggccagggt cagcaccagg
     8761 tagacccacg ggatcttgga ctggaagccg agggtcgcga acccggggcg gaacggcacc
     8821 gggatgccct cggacccgga cgtgaccgcg cgccagcggc tggccaggat caggaggacc
     8881 tgggagaagg cgatggtggc gaggccgaag tagggaccct tgaggcgatt cgcgaggtag
     8941 ccgatgacga ggccggccac ggtggcgagc agcgcgccca ccagcagccc gagccacggc
     9001 gactggttcc accgggtcgc gagcagggcc gccgagtagg cgccgagccc gaagaacgcg
     9061 gagtggccga gcgagacctg gcccgcgtag ccgccggcca cgttccacgc gcccgacacc
     9121 gcgccccaca ggaggatcag gatcaggctg tcgagcagga agccgtcgcg ggtcacgagc
     9181 ggggccagcg cggccaggcc gaagaggacg agggcgggga cgtcgcgctt cacgagttca
     9241 cgcgccgagc gtctcggacc cgcgctggcc gaagaggccg gcggggcgga acacgaggac
     9301 cgcgatgaag agcacgaagt agatgacttc cttccacgcg ctccccaggt agtaggagcc
     9361 gagcacctcg acccccgcca cgatcaggct gccgagcagg gccccgatca tgtcgccgag
     9421 gccgcccagc accaccacca cgtaggcgag cagcacgaac tggagcccgg cggtgggata
     9481 caccgagtag agcggcgaca tcaggacccc cgcgatgccg acgcagacca ggccgatcga
     9541 ccacgcgagc cggtagaccc ggtcggtgtc gatgcccatc agggtggccg cctccacgtc
     9601 ctgggcggtg gcccgcatga tgcggccggc ccgcgtccac ttcatgaagg ccaccagcgc
     9661 ggcggtgaac agcaccgcga ccaggaacgc caccacctgg gagaggttga aggcggcctc
     9721 gccgatccgg atcaccaccc cggagtgcca cggccgcacg aagcggaagt cgccggtcca
     9781 ggccaccagg gccgcgttct ggagcgcggt cgacaatccc acggtggtga agatctggac
     9841 gttgtggctc gccttgacca ccccgcgcat cacgagcgcg taggtgagga gcccgatggc
     9901 gaagaagagg ggcaccgcca ccagcaggat cacgtagggg tcgaggcccc agagggtgaa
     9961 cgaccagaag gcgaggtaca tccccagcat caggaactcg ccgtgggcga agttgacgac
    10021 gcgcatgatg ccgaagatga gggtcagccc caccgcgatc agggcgtaga tcccgcccag
    10081 gagcacggtg gagacgatca gctgggggac ggcgcccacc gggcgcggag cccgctactt
    10141 gcgctggtcc caggccggca gcggcatcca cttggcccgc agggtggaga actcctcggg
    10201 ccacaccgtc accatctggc cgttctgcca ctgcagcatg tagtgctgca cgcgctcgtt
    10261 ggcgttctgg ccgttctccg cgaacttgac cccccagccg ttgagcatgc tgcccaccgg
    10321 caggtccatc gacatcaccg cggagcggaa cttctcgagc tcgtcggtct tggcctggtc
    10381 gagcacgagc ttgagcagcc agaggccggc cccggcgagc cggtggctcg cgtagggctc
    10441 ggtgccggcc cgcttcttca ccgcctcggt gaagtcccgc tcgagcttct gggcctcggg
    10501 gcggaaggcc tccaggcgca gcccggtgct gggctcggcg agcacgaacg gcccgttgcc
    10561 ctcggcgccc agggccttgc cgaagtcggg ggagccgtag ccggtggcac ccgcgtgcac
    10621 gagggccttg aacatgaagt tctgctcgcg cgactggcgc cagaagagca ccgcgtcgtt
    10681 gccgcgcgcg atgtgatgga ccacgtccgg gttgacctgc ttgagcttgg ccacgatcgg
    10741 ggagaagtcg ttggtggagg cgaaggcgta gtactccatc gccacctcct gcatcccgaa
    10801 gtccttcttg gcccgctcgc gcgcggcctc ggtgaccccc tggccgtagg aggagtcctc
    10861 ggagaggaac gcgatcttga tctggttcgg cttcaggttg aagcgggggg cgaggtgctt
    10921 ggcgatgaac tcgacgttgt accagccgaa gccggtgccg tcgatctcgg tgcggaacac
    10981 gttcttgagg ccccgcttgc tgaagcgcgg gtccacgcag gaggactccc agtagatgac
    11041 gttctgccgc gaggccgcct cgctcgccgc gccgcagagg gtcgaggagt aggtcccggt
    11101 gatgatcttg acgccctcgc gggtgatgag gcggttggcc tcgctcgccg ccgcggtcgg
    11161 gtccaccccg tcgcccacca cgaagacgag cttcttgccc atgaccccgc cgcgctcgtt
    11221 gatcatgtcg cgcatgatct cgtagccctc gaggtgccgc ttgccctcga aggcgagcgc
    11281 gccggtcagc ggctcgagga cgccgatctt gtactcgccc tggagcgcct gcccctcggt
    11341 gccgcggatc agcgcggccg agaccagggt gacggcgatg agcagcacga gggcggtcag
    11401 gcgtccgcgg cgcttcgaca tgggggctcc tcctcgggat aggggatggc tcggcagttc
    11461 tagcatcgcg cccggcgcgg tgtcaacggt ccggcgatca ctgctcctcg gcctgctgct
    11521 gctcctgggc cgcgatctcc tcggcggcct gccggaggcg gtcgccgcgc cgctccggga
    11581 cctcctggta gaccggcacg tcgatctgct ccatctcctc gtgcagggcc gagatgtcct
    11641 tgggggtcga cgcctgggtc cgcacctcgg tcttcaaaat ttcgtccgcg ttgccgcgga
    11701 agatgccctt gttgggcggg acgtcgtcgt agtgccgccc gtggccgacg accacgtagt
    11761 gatcgtcgac cgggcggttg tgggtcgggt cgaagggcac ccagcccgcc gcgggcgagt
    11821 ggaactcgat ccacgcgtgg ctctgcgcca cctcgccctc ggccggcgac acgtggaggt
    11881 agccgctcac gtagcggcag gggatctggc ggagccgaag caggccgagc atgaggtggg
    11941 tgaagtcctg acagaccccg gcgcccaggc cgaggaagtc gtcggtggtg gagtcgtaac
    12001 gggtgacgtt cttgcggtag gtgaagtgct cct
//
LOCUS       DATMCV010000297         5045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_678340, whole genome shotgun sequence.
ACCESSION   DATMCV010000297 DATMCV010000000
VERSION     DATMCV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678340"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VNU03_06875"
     CDS             <1..268
                     /locus_tag="VNU03_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HWP74218.1"
                     /translation="FVVNLGELMARWTNDRWPATLHRVVNPPRAEALGSRRQSIVFFH
                     NPNYDAEIRCIPSCADPGRPAKYPVTTSGEHLKRQFTRTQTAAY"
     gene            complement(281..871)
                     /locus_tag="VNU03_06880"
     CDS             complement(281..871)
                     /locus_tag="VNU03_06880"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HWP74219.1"
                     /translation="MTVMWARGVLLALLVSGGTAAAESPPVVPYPGDYKTSLVRYAVV
                     DRIDGLSRDLYVSRAAIEALKRDPRLREFPVGVLFALDVHRAKLVARDHRTGAPTFAS
                     GPDGHLARSKDERTLHLMQKIRAGSGSQNWAFGGFDPVTGAPLKLELPGDCLLCHQAA
                     VVSDMTFSLTLLKRFAESGAVQYRLCAQPGRQVCPF"
     gene            916..1905
                     /locus_tag="VNU03_06885"
     CDS             916..1905
                     /locus_tag="VNU03_06885"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74220.1"
                     /translation="MERRRFLTLTAGGFLAASFAAEAQQADKVHRVGILGNVPISDPG
                     GARLWGAFADGLRALGYVDGRNIRIEHPSSEGRYERLPALAADLVRLKVDVIVAPAAQ
                     NVVAAKLATGTIPIVMVSVGDPVGNGLVASLARPGGNVTGTSFLTSAMVGKQLDLLKQ
                     ITPHGSRLAILLNPANPGHPLAREEAKVAAHSLGVQLQAVEARGPADFDNAFAAMTRE
                     RAGALFVPWDGTFLLHVVRIVQLAAKSRLAGMYGQRRYVDVGGLASYGPSAPESFRNA
                     AAYVDKILKGAKPGDLPVEQPTQFEFVINQKTAKALVLTISPPLLGRADEVIQ"
     gene            2022..4301
                     /locus_tag="VNU03_06890"
     CDS             2022..4301
                     /locus_tag="VNU03_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWP74221.1"
                     /translation="MPILKVFVDKDDRSAVLQQSTLIEPYDAFLLVQATPAGARRLAR
                     KYPVEDITDHYQVHLPNKTLNTARPRITTAGKVRHPAYKAERLSPGPHHYLVQFRGPI
                     KARWLAALKATGAKLREPEGSFAYVVWARKTSLPKIAALPFVRWMGHLPHASRVAPGL
                     ERPARARKILPRRRIRPNVYRVEVFDGRDTGRIAAAARRLRFVVLSQEPGARVLVLEG
                     ASSDGARARQIQALSAVHGVKFIRQQIVPRTTNNVATGVMGNAFAAASPTGPRLSGVG
                     EIIAVCDTGLDTGNPAAIHPDFAGRVVAIKSYPITSDWSSIITNPAGNDGPADLDSGH
                     GTHVSGSVLGDGTASVGGPALIRGHAHKAKLVFQAVEQEMKWKPSAPPNLSGERYLLA
                     GLPANLGPLFQFAYTSGARVHSNSWGGGDPGAYDDQCRQFDQFVWDHKDLCFVIAAGN
                     DGTDNDGDGKINLKSVTSPGTAKNCVTVGACENLRPEFDSETYGEWWPSDFPASPIKN
                     DPMANRPDQVVAFSSRGPTADNRVKPDVVAPGTFILSTRSSKVAANNFAWAAYPPDKA
                     HYFHMGGTSMATPLTSGAVALLREFLRKKQGIANPSAALLKALLIAGAKRLPGTAPST
                     AIVDNHQGFGRVNVDRSVKRALATLDGPALKTGTKSTFTVKVPTASNTLRIALCYSDF
                     PGSSLINNLNLIVTDPAGKRYVGNQPSSAGGTLTLDSTNNVEVVQVGKARKGTWTIEV
                     VASNVSAGPQDFALAVVLV"
     gene            complement(4337..>5045)
                     /locus_tag="VNU03_06895"
     CDS             complement(4337..>5045)
                     /locus_tag="VNU03_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HWP74222.1"
                     /translation="EKELTRRSDELARRRQALPWVRLDKEYRFETDEGSVSLAGLFRG
                     RSQLLVYHFMFGPDYKAGCPSCSAIADGFNGFAAHLAHHDVMLWAVSRAPLAKLQAYK
                     RRMGWTFPWASSFGGDFNPDFSVGFTEDQQRGGGIEYNYRREPAWQPREGSESADEGP
                     VAFAATTGTDVATYIRERPGVSAFALEDGVVYHAYSTYSRGVDSLWGMYQWLDRAPKG
                     RNETGLWWRRHDEYGKG"
BASE COUNT          827 a         1837 c         1632 g          749 t
ORIGIN      
        1 gttcgtggtc aacctcggcg agttgatggc gcgctggacc aacgaccgct ggccggccac
       61 gcttcatcgg gtggtgaacc caccgcgcgc ggaggcgctc ggcagccggc gccagtccat
      121 cgtgttcttc cacaacccga actacgacgc cgagatccgc tgcatcccga gctgcgccga
      181 cccgggtcgt ccggcgaagt acccggtcac gacgtcgggc gagcacctca agcgccagtt
      241 cacacggacg cagaccgcgg cctactgacc gccgcggcct tcagaagggg cagacctgtc
      301 gtccgggttg ggcgcagagc cgatactgca ccgccccgct ctcggcgaac cgcttcagca
      361 aggtcagaga gaacgtcatg tcgctcacca ccgcggcctg atggcacagg aggcagtcgc
      421 cgggcagctc gagcttcaat ggcgcgccgg tgaccgggtc gaaccctccg aacgcccagt
      481 tctgactgcc ggaccccgcc cggatcttct gcatcaggtg cagcgtgcgc tcgtccttgc
      541 tccgcgcgag atgaccgtcg gggccggacg cgaaggtggg ggcgcccgtc cggtggtccc
      601 gggcgaccag cttcgcgcgg tgaacgtcca gggcgaacag cacccccacc gggaactcgc
      661 ggaggcgcgg atcgcgcttc agggcttcga tcgccgcacg cgagacgtac agatctctcg
      721 agagcccgtc gatgcggtcc accaccgcgt acctcaccag gctggtcttg tagtcgccgg
      781 gatacggaac gaccggcggc gactccgccg ccgccgtgcc tcccgacacc agaagcgcca
      841 gcagcacccc acgggcccac atgacggtca tggagactat aaatcacccg actctgctag
      901 actccgccca ctcgcatgga gcgtcgaagg ttcctcaccc tgaccgcggg cggcttcctc
      961 gccgcgtcgt tcgccgccga ggcgcagcag gcggacaagg tccaccgcgt cggcatcctg
     1021 gggaacgtcc cgatctccga cccgggcggt gctcgtctct ggggggcatt cgcggacggg
     1081 ctacgggctc tcggctacgt ggacggccgg aacatccgga tcgagcaccc ctcatcggag
     1141 gggcggtacg agaggctccc ggcgctcgcg gccgacctgg tccgcctcaa ggtcgacgtc
     1201 atcgtggcgc ccgcggctca gaacgtggtg gccgccaagc tagccaccgg gacgatcccg
     1261 atcgtcatgg tcagtgtcgg cgacccggtg ggcaacgggc tcgtcgccag tctcgcgcgg
     1321 cccgggggga acgtcaccgg cacctccttc ctgacctcgg cgatggtcgg caagcagctg
     1381 gacctgctca agcagatcac tccccatggg tcgcggctgg ccatcctcct caacccggcg
     1441 aatcccggcc accccctcgc gcgggaggag gccaaggtgg cggcgcactc gctgggcgtc
     1501 cagcttcaag ccgtcgaggc gcgagggccg gccgacttcg acaacgcgtt cgcagccatg
     1561 acccgggagc gtgccggggc cctcttcgtg ccgtgggacg ggacgttcct cctgcacgtc
     1621 gtccggatcg tccagctcgc ggcgaagagc cgcctcgccg ggatgtacgg gcagcggcga
     1681 tacgtggacg tcggaggcct ggcctcctac ggaccgtccg ccccggagag ctttcgaaac
     1741 gccgccgcct acgtggacaa gattctcaag ggcgcgaagc ccggcgacct tccggtcgag
     1801 cagccaacgc agttcgagtt cgtgatcaac cagaagacgg ccaaagccct cgttctgacg
     1861 atttcaccgc cgctgctggg gcgcgcggac gaggtgatcc agtgagcgca gccgctgagc
     1921 ggtcggatcc tccccccggc gtcgtatcgc gttgacaccg tcgtcggggc gggtctagca
     1981 tcgcggcaag tggtcgacgc tccgcgaaca ggaggccgct catgccaatc ctgaaggtat
     2041 tcgtcgacaa ggacgatcgt tcggccgttc tccagcaatc cacgctgatc gagccgtacg
     2101 acgcgttctt gctcgtgcag gccacgccag ccggcgcgcg ccgcctcgcg cgcaagtatc
     2161 cggtcgagga catcacggac cactaccagg tccacctgcc gaacaagacc ctcaataccg
     2221 cgcgcccgcg catcaccacc gccggcaagg tgcgccaccc cgcctacaag gcggagcggc
     2281 tctcgccggg cccgcatcac tatctcgtgc agtttcgcgg ccccatcaag gcgcgctggc
     2341 tcgctgctct caaggcgacc ggggcgaaac tccgtgagcc ggagggcagc ttcgcctacg
     2401 tggtctgggc ccggaagacc tcgctgccga agatcgccgc gctgcctttc gtacggtgga
     2461 tggggcacct tccgcacgcc tcccgcgtcg cgccggggct cgagcggccc gcccgcgccc
     2521 gaaagatcct cccccgccgc cgcatccgcc cgaacgtcta tcgcgtcgag gtcttcgacg
     2581 gccgggacac cggaaggatc gcggccgccg cacgccggct tcgcttcgtc gtgctgtcgc
     2641 aggagccggg cgcccgcgtc ctcgtcctcg aaggcgccag ctccgacggg gcccgcgccc
     2701 ggcagatcca ggcgctctcc gccgtgcacg gcgtgaagtt catccgccag cagatcgtgc
     2761 cccgcaccac caacaacgtc gccaccgggg tcatggggaa cgccttcgcg gcggcgagcc
     2821 cgaccggtcc caggctgagc ggggtcggcg agatcatcgc cgtctgtgac accggcctcg
     2881 acaccggcaa ccccgccgcg atccacccgg acttcgccgg gcgcgtggtg gcgatcaaga
     2941 gctacccgat cacgtcggac tggagctcga tcatcacgaa tccggccggc aacgatgggc
     3001 ccgcggatct ggattcggga cacggcaccc acgtcagtgg atccgtcctc ggcgacggga
     3061 ccgcgtcggt gggcggcccc gccctcatcc ggggccacgc ccacaaggcc aagctcgtct
     3121 tccaggcggt ggaacaggag atgaagtgga agcccagcgc gccgcccaac ctatcgggtg
     3181 agcggtacct cctcgccggt cttcccgcga acctcgggcc cctcttccag tttgcctaca
     3241 ccagcggggc ccgcgtccac tcgaattcct ggggcggcgg cgacccggga gcgtacgacg
     3301 atcaatgccg gcagttcgac cagttcgtct gggaccacaa ggatctctgc ttcgtgatag
     3361 cggccggcaa tgacggcacc gacaacgacg gagacggcaa gatcaacctg aagagcgtga
     3421 cctcgccggg caccgccaag aactgcgtca cggtgggggc gtgcgagaat ctgcggcccg
     3481 agttcgattc cgagacgtat ggagaatggt ggccctcgga ttttcccgcg agccccatca
     3541 agaacgatcc gatggcgaat cgccccgatc aggtcgtggc cttcagcagc cgtggaccga
     3601 ccgcggacaa tcgcgtgaag ccggacgtgg tcgctccggg caccttcatc ctgtccaccc
     3661 gctcctcgaa ggtggccgcg aacaacttcg cgtgggccgc ctatccgccg gacaaggcgc
     3721 actacttcca catgggcggc accagcatgg cgacccctct cacctcggga gcggtggcct
     3781 tgctgcgtga gttcctgaga aagaagcagg gcatcgccaa cccctcggcg gctctcctca
     3841 aggccttgct catcgcgggg gcgaagcgct tgccgggaac cgcgccgagc accgcgatcg
     3901 tcgacaacca ccagggcttc ggtcgggtga acgtcgaccg gtcggtcaag cgagcgctcg
     3961 ccaccctgga cgggcccgcg ctcaagaccg gcaccaagtc gacgttcacg gtcaaggtcc
     4021 ctaccgcgag caacacgctg cggatcgcgc tctgctactc agacttcccg ggatcctcac
     4081 tgatcaacaa cctgaacctg atcgtgacgg atccggccgg gaagcgatac gtcggcaatc
     4141 agccatcctc ggccggaggc accctgaccc tcgattcgac gaacaacgtc gaggtggtcc
     4201 aggtggggaa ggccaggaag ggcacctgga ccatcgaggt cgtcgcctcg aacgtcagcg
     4261 ccggccccca ggacttcgcc ctggcggtgg tcctggtgta gccgcctatc ctccccgccc
     4321 gcccggcggg ccgcgctcaa cccttgccgt actcgtcgtg gcggcgccac cagaggcccg
     4381 tctcgttgcg ccccttgggg gcgcggtcga gccactggta catgccccag aggctgtcca
     4441 ctccgcgcga ataggtggaa taggcgtggt agacgacgcc gtcctcgagc gcgaacgcgc
     4501 tcacgccggg cctctcgcgt atgtacgtgg ccacgtcggt tccggtggtg gccgcgaagg
     4561 cgaccggtcc ctcgtcggcg ctctccgaac cctcgcgcgg ctgccaggcc ggctcgcgcc
     4621 ggtagttgta ttcgatacct cccccgcgct gttggtcctc ggtgaacccg acgctgaagt
     4681 cggggttgaa gtcaccgccg aacgaggacg cccagggaaa cgtccacccc atccgccgct
     4741 tgtacgcctg cagtttcgcg agcggcgccc gcgacaccgc ccagagcatg acgtcgtgat
     4801 gcgccaggtg cgcggcgaac ccgttgaagc cgtccgcgat cgccgagcag gacggacacc
     4861 ccgccttgta gtccggcccg aacatgaagt ggtagaccag aagctgcgag cgcccccgga
     4921 agaggccggc cagcgaaacg ctcccctcgt cggtctcgaa tcggtactcc ttgtcgagcc
     4981 gcacccacgg cagcgcctga cgccgtcgcg ccagctcgtc gctacgccgc gtcagctcct
     5041 tctct
//
LOCUS       DATMCV010000298         3544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_881097, whole genome shotgun sequence.
ACCESSION   DATMCV010000298 DATMCV010000000
VERSION     DATMCV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881097"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VNU03_06900"
     CDS             complement(<1..895)
                     /locus_tag="VNU03_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HWP74223.1"
                     /translation="MNFLSPFALLLGTLAVPLLLLYFLKVRRRQMPVSSLLLWQAALR
                     DREASTFFQRLQRDPLLILQILALLALTVALARPAVTVMGQGAKRVAIVMDTSASMKA
                     TDVSPSRFVQAQRAALGLLGRLGEGAEVMVVEAGVQPKVVVPFTREHDRVASALRSME
                     GHDLPNRLAEGLRTARALVGSDPRAEIHVFTDGAHPEAAQAQGDDVRVRWTGVGRRSH
                     NVGITSLAVRRNYFGTYNSQAFFSVGNFSSERQSFSLRLTLDDETLTERTLTLDPQVR
                     RAIVVPFSEQRGGVLKLRLDVS"
     gene            complement(892..1785)
                     /locus_tag="VNU03_06905"
     CDS             complement(892..1785)
                     /locus_tag="VNU03_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWP74224.1"
                     /translation="MATVRAGQELLSADFLSQLERLSLLSRRSFRGRVKGERRSPRKG
                     ISVEFSDYRPYGVGDDLRYVDWNVYARLDRLYLKLFVDEEDLCLHLLVDASASMGFGD
                     PSKLDHAARLAAALGFVGLVGLERVGVGVVRERMAEGFNPARGRSQVLPLMNFLGRLE
                     PGGATSLNEGLAAYALRSREAGLAVLVSDLMDPGGYERGLKALLERRFDVHVIHVLAP
                     DEMNPGFGGDLRLLDAETNELRDLTLDAEALRGYHQRLREFLERAEQFCRGNEIAYHR
                     VVTDSPVEEFMLRQLKGLLLA"
     gene            complement(1790..3274)
                     /locus_tag="VNU03_06910"
     CDS             complement(1790..3274)
                     /locus_tag="VNU03_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74225.1"
                     /translation="MPGPTVIERLVADVARQIRLRRAEFYGLRGLFWGAVAAVLPLLL
                     KESLGPWSYVGAVLCLLAGAAAGAGYGVLLKLPAAEAARLADRGYGLQERISTALEWA
                     ERPDRTPIVDALVADATTRAGQLGARRIIPRRVPREARLVPVPLLLGLVLAAAPPIPL
                     PQARLPNFSVSREEDEEKAAVDRSGEAQQAERKQATRRDQVQRTDVQERNLSPRLGGG
                     GQSQPGDLAAVFKDTSLGGRAPDFNSFLKKGDERLRMLDSLERLPDLQSDFTQSQARM
                     VFQKAKALRGGLDPNKKVSPEKLRELLSEMERLGRKNGGQGQNNWSGDAYEGMEALEG
                     GQTDKAMEAMERALNKMRSMEERGRDGKGLKGGRESERRGGKRGDKGSGQPGGPGDEG
                     DFPEGEGLLPGRGKSGAPKGDATARLRGSPFDVGVEGEARAGRKQGIDTNMVGRGAQM
                     PSRLQYMGVLGQYRKMMEESIAREQVPRDFQSQVKEYFQSLDEK"
     gene            complement(3289..>3544)
                     /locus_tag="VNU03_06915"
     CDS             complement(3289..>3544)
                     /locus_tag="VNU03_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP74226.1"
                     /translation="YIRYGASPRGAQALVLGAKVRALSDGRFNVSVDDLRAMALPALR
                     HRLILNFEGEAAGVDVDDLVGQIVESAEAMSGTGKEVFLR"
BASE COUNT          495 a         1336 c         1217 g          496 t
ORIGIN      
        1 cggagacgtc gagccggagc ttgaggaccc cgccccgctg ctcgctgaag ggcaccacga
       61 tcgcgcgccg cacctggggg tcgagggtga gggtgcgctc ggtgagggtc tcgtcgtcga
      121 gggtcaggcg gagggagaag ctctgccgct cggaggagaa gttccccacc gagaagaagg
      181 cctgcgagtt gtaggtgccg aagtagttgc ggcgcaccgc gaggctcgtg atgcccacgt
      241 tgtgggagcg gcggcccacc ccggtccagc gcacccgcac gtcgtcgccc tgcgcctggg
      301 cggcctcggg gtgcgccccg tcggtgaaga cgtggatctc ggcgcgcgga tccgagccga
      361 ccagggcccg cgcggtgcgg agcccctccg cgagccggtt cggcaggtcg tgcccctcca
      421 tcgaccggag ggcggaggcc acccggtcgt gctcgcgggt gaacggcacc accaccttcg
      481 gctgcacgcc cgcctcgacc accatcacct cggcgccctc acccaggcgc ccgagcagcc
      541 ccagcgcggc gcgctgcgcc tgcacgaagc gcgacggaga gacgtcggtg gccttcatgg
      601 acgccgaggt gtccatgacg atggcgaccc gcttcgcccc ctgccccatc acggtcaccg
      661 cgggccgggc cagcgcgacg gtgagcgcga gcagggccag gatctgcaag atcaggagcg
      721 gatcgcgctg caggcgctgg aagaaggtgg aggcctcgcg gtcgcgcagg gccgcctgcc
      781 agagcaagag gctcgagacc ggcatctgcc ggcggcgcac cttcaggaag tacaggagca
      841 gcagcggcac cgcgagggtg ccgagcagca gcgcgaacgg gctcaggaag ttcatgccag
      901 cagcagcccc ttgagctggc ggagcatgaa ctcctccacc gggctatcgg tcaccacgcg
      961 gtgataggcg atctcgttcc cgcggcagaa ctgctcggcg cgctcgagga actcgcgcag
     1021 ccgctggtgg taaccgcgca gggcctcggc gtcgagcgtc aggtcgcgca gctcgttggt
     1081 ctccgcgtcg agcagccgca ggtccccgcc gaagccgggg ttcatctcgt cgggggcgag
     1141 cacgtggatg acgtgcacgt cgaagcgccg ctcgagcagg gccttgagcc cgcgctcgta
     1201 cccgcccggg tccatcaggt ccgagacgag cacggccagg cccgcctcgc gcgagcggag
     1261 cgcgtaggcg gcgagcccct cgttgagcga ggtcgccccg ccgggctcga gccgtcccag
     1321 gaagttcatg agcggcagca cctggctgcg cccgcgggcg gggttgaagc cctccgccat
     1381 ccgctcccgc accacgccca ccccgacgcg ctcgagcccc accaggccca cgaagcccag
     1441 cgcggcggcc agtcgcgcgg cgtggtcgag cttggagggg tctccgaagc ccatggaggc
     1501 ggaggcgtcc accaggaggt gcagacagag atcctcctcg tcgacgaaga gcttgaggta
     1561 gagccgatcg agacgggcgt agacgttcca gtcgacgtag cgcaggtcgt cgccgacgcc
     1621 gtacgggcgg tagtcggaga actcgacgct gatgcccttg cgcgggctac ggcgctctcc
     1681 cttgacccgc ccgcggaacg agcggcggga gagcaggctc agacgctcga gctggctgag
     1741 gaagtcggcg gacagcagct cctggcccgc gcggacggtg gccacgccgc tatttctcat
     1801 ccagcgactg gaagtactcc ttcacttgac tctggaagtc gcgcggcacc tgctcccgcg
     1861 cgatggactc ttccatcatc ttccggtact gcccgagcac gcccatgtac tgcagccgcg
     1921 acggcatctg ggcgccgcgc cccaccatgt tggtgtcgat gccctgcttc cgcccggccc
     1981 gcgcctcgcc ctcgaccccc acgtcgaatg ggctgccccg cagccgcgcg gtcgcgtcgc
     2041 ccttcggtgc cccgctcttg ccgcgccccg gcagcaggcc ctcgccctca ggaaaatcac
     2101 cctcgtcccc cggcccgccc ggctgcccgc tgcccttgtc gccgcgcttg ccgccccggc
     2161 gctcgctctc ccgcccgccc ttcaggccct tgccgtcgcg gccgcgctct tccatcgagc
     2221 gcatcttgtt gagcgcgcgc tccatcgctt ccatcgcctt gtcggtctgc ccgccctcga
     2281 gcgcttccat gccctcgtag gcgtcgcccg accagttgtt ctggccctgc ccgccgttct
     2341 tgcgcccgag ccgctccatc tcgctcagca gctcgcgcag cttctcgggc gagaccttct
     2401 tgttcgggtc gagcccgcct cttagcgcct tggccttctg gaagaccatg cgggcctggc
     2461 tctgggtgaa atcactctgg agatccggca gccgctcgag cgagtcgagc atgcggagac
     2521 gctcgtcgcc cttcttgagg aacgagttga agtcgggcgc gcgcccgccc agggaggtgt
     2581 ccttgaatac cgcggccagg tcgccgggct ggctctgccc gccgccgccc aggcgcgggc
     2641 tcaggttgcg ctcctgcacg tcggtgcgct gcacctggtc gcgccgggtg gcctgcttgc
     2701 gctccgcctg ctgggcctcc ccgctgcggt cgaccgccgc cttctcctcg tcctcctcgc
     2761 gggagactga gaagttgggc agacgggcct ggggaagcgg gatcggcggc gcggccgcga
     2821 gcacgagccc gagcagcagg gggaccggca cgagccgggc ctcgcgcgga acccgccgcg
     2881 ggatgatgcg ccgggcgccc agctgcccgg cccgcgtcgt cgcgtcggcc accagcgcat
     2941 ccacgatcgg cgtgcggtcg gggcgctccg cccactcgag cgcggtgctg atccgctcct
     3001 gcagcccgta gccgcgatcg gcgagccggg ccgcctcggc ggcggggagc ttgaggagga
     3061 caccgtagcc ggcgcccgcg gccgcgccgg cgagcaggca cagcaccgcg cccacgtagc
     3121 tccacgggcc cagcgactcc ttgagcagga gcggcagcac cgcggccacc gcgccccaga
     3181 acagcccgcg caggccgtag aactccgccc gccgcagccg gatctgccgg gcgacgtccg
     3241 cgaccagccg ctcgatcacc gtgggaccgg gcatcgactg cctcctctct acctcaggaa
     3301 cacctctttc cccgtcccac tcatcgcctc ggcggactcg acgatctggc ccacgaggtc
     3361 gtccacgtcc acccccgccg cctcaccctc gaagttcaag atgaggcggt gacgcagggc
     3421 gggcagcgcc atcgcccgca gatcgtcgac actgacattg aaccggccgt cggacagagc
     3481 gcgaaccttc gcccccagca ccagcgcctg cgcgccgcgc gggctcgccc cgtagcggat
     3541 gtag
//
LOCUS       DATMCV010000299         8022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_750476, whole genome shotgun sequence.
ACCESSION   DATMCV010000299 DATMCV010000000
VERSION     DATMCV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750476"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2902
                     /gene="carB"
                     /locus_tag="VNU03_06920"
     CDS             <1..2902
                     /gene="carB"
                     /locus_tag="VNU03_06920"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWP74227.1"
                     /translation="GVELIGAKLPAIKTAEDRNLFGAAMARIGLAMPLGFYASSPEEA
                     LAGMRQRRMQFPLIIRPSFTLGGTGGSVAYHPEELEAAIKWGLQQSPLQQVLVEESVI
                     GWKEFELEVMRDLKDNVVIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQLMRDASL
                     AIIREIGVETGGSNIQFGINPVDGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLA
                     VGYTLDELRNDITRETPASFEPAIDYCVVKIPRWAFEKFPEADQTLTTQMKSVGEVMA
                     IGRTFKEALQKAVRSLEQDRWGLTLDRPVADLDELRQKIRIPNADRVFAIGEAYRRGL
                     GTRELHELSAIDPWFLDNIRQIVEYEIVITAAGLGDLDVLRRAKQMGFADRRIAQLTS
                     TTEPEVRQARLQAGIRPVYKMVDTCAAEFVAHTPYLYSTYEEEDEAPPTSREKVVILG
                     SGPNRIGQGIEFDYACVHAAFALREAGVEAIMVNCNPETVSTDYDTSDRLYFEPLTFE
                     DVMSIVEREQPRGVIVQFGGQTPLKLVVPLEQAGVPILGTPPDAIDRAEDRRRFSDLL
                     DRLGLAQAPGSTARSFEEAARIAANIGYPVLVRPSYVLGGRAMQIVYDETDLQGYMKE
                     AVRVSPEHPILVDKFLEDALEIDVDAIADGERVVVGGVMEHVEKAGIHSGDSACALPP
                     YSLGDDQIERIQAQTRALARELGVIGLLNIQFAIKNEQVFVLEVNPRASRTVPFVSKA
                     IGVPLAKLATRVMLGSTLASLGFAEEREVSHIAVKEAVFPFIKFPGVDTILGPEMKST
                     GEVMGIDYDFRKAYVKSQLSAGSPLPTSGKVFLSVKNRDKRAVLQIAKRLAEMGFSLV
                     ATVGTAKLLQRQGMTVEVVHKVNENYRPNIVDLMKRGEIALVFNTPEDGRARRDSYLI
                     RRSAVTQNISYYTTVDGAQAAIGGIEALLKGEISVKPLQEYYHPLPPGERAG"
     gene            2911..3639
                     /gene="pyrF"
                     /locus_tag="VNU03_06925"
     CDS             2911..3639
                     /gene="pyrF"
                     /locus_tag="VNU03_06925"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWP74228.1"
                     /translation="MHPPTHVKDRLIVALDVETLADAERMVDRLEGLATRFKIGSQLF
                     TAAGPGAVEAIQKRGAEVFLDLKFHDIPNTVAGAAREATRMGVLMFNVHASGGRAMMA
                     AAAEGAATAARELGGRRPIVLAVTVLTSLDRAALSRELGVADSVEGHVLHLAVLAAAA
                     GLDGCVASPNEVGALRTSRGAGWVIVTPGVRPAGSAMGDQSRIATPGAAVSAGAHYLV
                     VGRPITAAPDPARAAEAVLREMGA"
     gene            3671..4285
                     /gene="pyrE"
                     /locus_tag="VNU03_06930"
     CDS             3671..4285
                     /gene="pyrE"
                     /locus_tag="VNU03_06930"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWP74229.1"
                     /translation="MSAGDAPAALIRRLFEIGAIRFGEFTLKSGIVSPFYIDLRVVIS
                     FPDVLEQIGALIAALVSRCGGDRVGGIPYAGLPLAVAASLAGRVPLIYPRREEKGYGT
                     RKRIEGQFKPGERVVLIDDIITDGASKLEAIAPLEEAGLVVRDLVILVDREQGGRELL
                     AARGYTLHAVLTISQCFDEGERASLAPVALIRQSREFLQATRFA"
     gene            4285..4815
                     /gene="hpt"
                     /locus_tag="VNU03_06935"
     CDS             4285..4815
                     /gene="hpt"
                     /locus_tag="VNU03_06935"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWP74230.1"
                     /translation="MPAASDGPGEILVEREVIQADVARLAAQISADYQDRPVHLIGVL
                     KGSAVFLSDLMRAITVPVTVDFISVGAYGPTITASGVVRIRKDLDEPIEGRDIVVVEG
                     ICASGRTLAYLLRNFQTRNPASIRVCALLAKQCARAQELTLHYVGREIPDVFVVGYGL
                     DRDERFRNLPHVARLA"
     gene            4867..5502
                     /locus_tag="VNU03_06940"
     CDS             4867..5502
                     /locus_tag="VNU03_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74231.1"
                     /translation="MRLRRPLQAAAGLLATVALSIVLAGCESGGISRTVHIAERPIWT
                     AGNSWTYRGKGPNGTYTVTRKVLSEGIFEGRDCYQIEGGDARYWYTKQLGYLARTSGD
                     KTVRLAAPPEDWQWPLQVGKQWSATVTWTDKGEQERTFVLTGVWLVETYEEVKTPAGT
                     FKAFKVSRREVESGASQEFWYSPEARGWVKIKGINTADGNYEEELTAYTAR"
     gene            complement(5506..6321)
                     /locus_tag="VNU03_06945"
     CDS             complement(5506..6321)
                     /locus_tag="VNU03_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74232.1"
                     /translation="MTRDRLRVLGWQVLILAVVLVGWQYLTGIKAVSKTPGLYWIDPF
                     FISRPSAIAERFVYLMSPRVRLTIWQMALSTVQSTVWGFLAGVSTGFVAGLVLGRGDR
                     LARVFQPYIVAFNSLPRIALVPLITMIFGFGLLAKIVLAWSIVFFIVFFNTFQGARSV
                     DADLIHSARFLGASERQIMTSVIVPSALAWTFASLTPSISFALIGVVVGEFIGGESGG
                     GLGYLIIQSLGTLNAADMMVALFVLGTIGIVMALGIKQLEARLLRWRPENRQR"
     gene            complement(6479..7243)
                     /locus_tag="VNU03_06950"
     CDS             complement(6479..7243)
                     /locus_tag="VNU03_06950"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74233.1"
                     /translation="MRDLSKTFGQGPREVPAVSPVSFDVHEREFVAIVGPSGCGKSTI
                     LNMIGGLVAPSGGEIVVDGLRVTGAPPPQVGYVFQKDTVFPWRTVERNIALGLEYRHV
                     APAARAARVREAIALAGLQGFEDAFPATLSGGMRQRVALMRSLVVDPEILLMDEPFGA
                     LDTHTKLNLHAELLAIWQARQQTVIFVTHDLSEAITLADRIVVMTRRPGRIKLIYDVN
                     LPRPRDVIRVRETAEYSREYGEIWHALGEEFRPVAT"
     gene            complement(7278..>8022)
                     /locus_tag="VNU03_06955"
     CDS             complement(7278..>8022)
                     /locus_tag="VNU03_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74234.1"
                     /translation="FATDTRCSYANIVVRKELWDKGVKTVEALADGKLVGRKAVVAAT
                     AIGSGTYVYGVYVLQNIKGADGRPVNDHVEWVGGGASSTMLGGLKAGKFDAIMAVPEW
                     QAAAVEEDFGKPIYDIRDEKAWNRVFGGPIPVTVGYVLKETIEKSPDLVQAYVNANYR
                     AQQWIRKAKDDEVTDLLWKPYMDAFKREVVLESVRYYRTIFDWDFVIEERDYERGMKV
                     WVPTAVDKPIPYKQAVDMSFVKKAQARYK"
BASE COUNT         1278 a         2794 c         2773 g         1177 t
ORIGIN      
        1 cggggtcgag ctgatcgggg ccaagctgcc cgcgatcaag accgcggagg accgcaacct
       61 cttcggcgcc gcgatggcgc gcatcgggct cgcgatgccc ctgggcttct acgcgtcgag
      121 cccggaggaa gcgctcgccg gcatgcggca gcggcggatg cagttccccc tcatcatccg
      181 gccctccttc accctcggcg gcaccggcgg atcggtcgcc tatcacccgg aggagctcga
      241 ggccgcgatc aagtggggac tccagcagag cccgctccag caggtgctgg tggaggagtc
      301 ggtcatcggg tggaaggagt tcgagctcga ggtgatgcga gacctgaagg acaacgtggt
      361 catcgtctgc tcgatcgaga acttcgatcc gatgggagtg cacaccggcg actcgatcac
      421 ggtggcgccg gcccagacgc tcaccgacaa ggagtaccag ctcatgcggg acgcctccct
      481 cgcgatcatc cgcgagatcg gcgtcgagac cggcggctcc aacatccagt tcgggatcaa
      541 cccggtggac ggccgcatgg tggtgatcga gatgaacccg cgggtgtcgc gctcctccgc
      601 gctcgcctcc aaggccaccg gcttcccgat cgccaagatc gcggccaagc tcgcggtggg
      661 ctacaccctg gacgagctgc gcaacgacat cacccgcgag acgcccgcgt cgttcgagcc
      721 cgccatcgac tactgcgtgg tgaagatccc gcgctgggcc ttcgagaagt tccccgaggc
      781 ggaccagacc ctgaccaccc agatgaagtc ggtgggcgag gtcatggcga tcgggcgcac
      841 cttcaaggag gcgctccaga aggcggtgcg ctcgctggag caggaccgct gggggctcac
      901 cctggaccgg ccggtcgccg acctcgacga gctgcgccag aagatccgca tcccgaacgc
      961 ggaccgcgtc ttcgcgatcg gcgaggccta ccgccgcggg ctcggcaccc gtgagctcca
     1021 cgagctctcc gcgatcgacc cgtggttcct cgacaacatc cggcagatcg tggagtacga
     1081 gatcgtcatc accgcggcgg gcctgggtga cctcgacgtc ctgcggcggg ccaagcagat
     1141 gggcttcgcg gaccggcgga tcgcgcagct cacctcgacc accgaacccg aggtccggca
     1201 ggcgcgcctc caggccggca tccggccggt ctataaaatg gtcgacacct gcgcggccga
     1261 gttcgtggcc cacactccct acctctactc gacctacgag gaggaggacg aggcgccgcc
     1321 gacgtctcga gagaaagtgg taatcctggg aagcgggccc aaccgcatcg gccagggcat
     1381 cgagttcgac tacgcgtgcg tacacgccgc cttcgcgcta cgggaggcgg gcgtggaagc
     1441 gatcatggtc aactgcaacc cggagacggt gtcgaccgac tacgacacct ccgatcgcct
     1501 ctacttcgag ccactcacgt tcgaggacgt gatgagcatc gtggagcgcg agcagccccg
     1561 cggggtgatc gtgcagttcg gcggccagac cccgctgaag ctggtggtgc cgctcgagca
     1621 ggccggcgtg ccgatcctcg gcacgccgcc cgacgccatc gaccgcgcgg aggaccgccg
     1681 ccgcttctcc gacctcctcg accggctggg gctcgcccag gcccccggct ccaccgcgcg
     1741 ctcgttcgag gaggcggcgc ggatcgcggc caacatcggc tacccggtgc tggtgcggcc
     1801 ctcctacgtc ctgggcgggc gcgccatgca gatcgtttac gacgagaccg atctccaggg
     1861 ctatatgaag gaagcggtgc gggtcagccc ggagcacccg atcctggtcg acaagttcct
     1921 cgaggacgcc ctcgagatcg acgtggacgc catcgcggac ggtgagcggg tggtggtggg
     1981 cggggtcatg gagcacgtgg agaaggccgg catccactcg ggcgactccg cgtgcgcgct
     2041 gccgccctac tcgctcggcg acgaccagat cgagcgcatc caggcccaga cccgagcgct
     2101 ggcccgcgag ctgggggtga tcggcctcct caacatccag ttcgcgatca agaacgagca
     2161 ggtcttcgtg ctcgaggtca acccgcgcgc ctcccgcacg gtgcccttcg tctccaaggc
     2221 gatcggggtg ccgctcgcca agctggccac ccgggtcatg ctcgggtcga cgctggcctc
     2281 gctcggcttc gccgaggagc gcgaggtctc gcacatcgcg gtgaaggaag cggtgttccc
     2341 gttcatcaag ttcccgggcg tcgacaccat cctgggaccg gagatgaagt cgaccggcga
     2401 ggtgatgggc atcgactacg acttccggaa ggcctacgtc aagtcgcagc tctcggccgg
     2461 gtcgccgctg cccacctccg gcaaggtctt cctctcggtg aagaaccggg acaagcgcgc
     2521 ggtgctgcag atcgccaagc ggctggccga gatgggcttc agcctggtgg ccaccgtcgg
     2581 caccgccaag ctcctgcagc gacagggcat gacggtggag gtcgtgcaca aggtcaacga
     2641 gaactaccgg cccaacatcg tcgacctcat gaagcgcggc gagatcgcgc tcgtgttcaa
     2701 cacccccgag gacgggcggg cccgccgcga ctcctacctg atccgccgga gcgcggtgac
     2761 ccagaacatc tcctactaca ccaccgtcga cggagcgcag gccgccatcg gcggcatcga
     2821 ggcgctgctc aagggcgaga tctccgtcaa gccactccag gagtactacc atccgctccc
     2881 tcccggggag cgggccggct gaggcccccc atgcatccgc ccacccacgt caaggaccgc
     2941 ctgatcgtcg cgctcgacgt cgagaccctg gccgacgcag agcggatggt cgaccgcctc
     3001 gaggggctcg ccactcgctt caagatcggc tcgcagctct tcaccgccgc cgggcccggc
     3061 gcggtcgagg ccatccagaa gcggggcgcc gaggtcttcc tcgacctcaa gttccacgac
     3121 atccccaaca cagtggcggg cgccgcgcgc gaggcgaccc ggatgggcgt gctgatgttc
     3181 aacgtccacg cctccggcgg gcgcgccatg atggcggccg cggccgaggg ggccgcgacc
     3241 gcggcccggg agctgggagg ccggcggccg atcgtcctcg cggtcaccgt gctcacgagc
     3301 ctcgaccggg ccgcgctgag ccgcgagctc ggcgtggccg actcggtgga gggccacgtg
     3361 ctccacctgg ccgtcctcgc cgccgcagcg gggctggacg gctgcgtggc gtcgcccaac
     3421 gaggtcggcg cgctccggac cagccggggg gcgggctggg tgatcgtgac gccgggcgtg
     3481 cggccggcgg gcagcgcgat gggtgaccag tcccgcatcg ccacgccggg ggcggccgtc
     3541 tccgccggcg cgcactatct cgtggtcggg cgcccgatca ccgccgcgcc cgacccggcc
     3601 cgcgccgcgg aagcggtgct gcgggagatg ggcgcatgag cacgaccgcg gtgccgcggg
     3661 agatgggcgc atgagcgccg gggacgcgcc cgcggccctg atccggcggc tcttcgagat
     3721 cggggcgatc cgcttcggcg agttcacgct caagtccggc atcgtctcgc ccttctacat
     3781 cgacctccgc gtggtcatct ccttccccga cgtcctcgag cagatcggcg cgctcatcgc
     3841 cgccctggtg agccggtgtg gcggcgatcg ggtgggcggc atcccctacg cggggctgcc
     3901 cctggcggtg gcggccagcc tggccgggcg cgtcccgctc atctacccgc gacgcgagga
     3961 gaagggctac ggcacccgga agcgcatcga gggacagttc aagcccggcg agcgcgtggt
     4021 gctcatcgac gacatcatca ccgacggggc gagcaagctc gaggcgatcg ccccgctcga
     4081 ggaggcgggc ctggtggtcc gggacctcgt catcctggtg gatcgcgagc agggcggtcg
     4141 cgagctgctc gccgcgcgcg gctacacgct gcacgcggtg ctcaccatct cccagtgctt
     4201 cgacgaggga gagcgggcct cgctggcccc ggtggccctg atccgccagt cgcgcgagtt
     4261 cctccaggcc acccggttcg cctgatgccg gcggcgagcg atggtccggg cgagatcctc
     4321 gtcgagcgcg aggtgatcca ggccgacgtc gcccggctcg ccgcccagat ctcggccgac
     4381 taccaggacc ggccggtgca cctgatcggg gtgctcaagg gctccgccgt cttcctctcc
     4441 gatctcatgc gggcgatcac ggtcccggtc acggtggact tcatctcggt gggggcctat
     4501 ggcccgacca tcaccgcctc gggcgtggtg cgcatccgga aggacctgga cgagccgatc
     4561 gagggccgcg acatcgtggt cgtcgagggc atctgcgcga gcgggcgcac cctggcctat
     4621 ctcctccgca acttccagac ccgcaacccg gcctcgatcc gggtctgcgc gctcctggcc
     4681 aagcagtgcg cccgggccca ggagctgacc ctgcactacg tgggccgcga gatcccggac
     4741 gtgttcgtgg tcggctatgg cctcgaccgg gacgagcgat ttcgcaacct gccccacgtc
     4801 gcccgcctcg cctaggcccc ttcgtaggtg tccggttgtt tggcgcactt gcggtatgct
     4861 ttcctggtga gactccgacg accactccag gccgcggccg ggctcctggc caccgtcgcc
     4921 ctctcgatcg tgctcgccgg gtgcgagagc ggcggcatct cccgcaccgt ccacatcgcg
     4981 gagcggccga tctggaccgc gggcaactcg tggacctacc ggggcaaagg cccgaacggg
     5041 acgtacacgg tcacccgcaa ggtgctgagc gagggaatct tcgagggccg cgactgctac
     5101 cagatcgagg ggggcgacgc ccgctactgg tacaccaagc agctcggcta cctggcccgg
     5161 acgagtggag acaagacggt gcggctggcc gcgccgcccg aggactggca gtggccgctc
     5221 caggtcggca agcagtggtc ggccaccgtg acgtggacgg acaagggtga gcaggagcgg
     5281 accttcgtgc tgaccggcgt gtggctggtg gagacctacg aggaggtcaa gaccccggcc
     5341 ggaacgttca aggccttcaa ggtctcccgc cgcgaggtcg agagcggcgc ctcccaggag
     5401 ttctggtaca gcccggaagc caggggctgg gtcaagatca agggcatcaa caccgccgac
     5461 ggcaactacg aggaagagct gaccgcctac accgcgcgct gatcctcagc gctgccggtt
     5521 ctccggccgc caccgcagca ggcgcgcctc gagctgcttg atccccagcg ccatcacgat
     5581 gccgatcgtg cccagcacga agagcgccac catcatgtcc gccgcgttga gggtgccgag
     5641 cgactggatg atcaggtagc cgagcccgcc tcccgattcg cccccgatga actcgcccac
     5701 caccactccg atgagggcga acgagatgga gggggtcagc gacgcgaagg tccaggcgag
     5761 cgcggagggc acgatcacgc tggtcatgat ctggcgctcg gaggcgccca ggaagcgcgc
     5821 ggagtggatg aggtcggcgt cgacgctccg cgcgccctgg aaggtgttga agaagacgat
     5881 gaagaacacg atgctccagg ccagcacgat cttggcgagc agaccgaagc cgaagatcat
     5941 cgtgatgagc ggcaccagcg cgatgcgggg cagcgagttg aaggccacga tgtagggctg
     6001 gaacacgcgg gccagccggt cgccgcgccc gagcaccagg cccgccacga agccggtgga
     6061 gacgccggcg aggaagcccc agacggtgga ttgcacggtc gagagcgcca tctgccagat
     6121 ggtgagccgt acccggggcg acatcaggta gacgaaccgc tccgcgatcg cggagggccg
     6181 gctgatgaag aacggatcga tccagtagag ccccggcgtc ttcgacaccg ccttgatccc
     6241 ggtcaggtac tgccagccga ccagcaccac cgccaggatc agcacctgcc agccgagcac
     6301 gcggaggcgg tctcttgtca tggccgccgg gagtaagcgg ttgtccgcaa cccggggtga
     6361 acggttgtcc gccgctgggg gagtgcgggg gccatttctg ggcccccgca gatgaaagag
     6421 gggcgggttc gtgtgtacgg agcacgccgg gtcgccccga ccgagatgtt tcgcctgatc
     6481 atgtggccac cggccggaat tcttcgccca gcgcgtgcca gatctcgccg tactcccgtg
     6541 aatattccgc ggtctcgcgc acgcggatca cgtcgcgggg gcgcgggagg ttcacgtcgt
     6601 agatgagctt gatgcggccg gggcggcggg tcatcaccac gatgcggtcg gccagggtga
     6661 tggcctcgga gagatcgtgg gtgacgaaga tcacggtctg ctgcctggcc tgccagatcg
     6721 cgagcagctc ggcgtggagg ttcagcttgg tgtgggtgtc gagcgcgccg aagggctcgt
     6781 ccatcagcaa gatctccggg tcgaccacca gcgaccgcat cagcgccacg cgctgccgca
     6841 ttccgccgga gagcgtggcc gggaacgcgt cctcgaagcc ctgcagcccg gccagcgcga
     6901 tggcctcgcg cacgcgcgcg gcgcgcgcgg cgggagccac gtggcggtac tcgaggccga
     6961 gcgcgatgtt gcgctcgacg gtccgccagg gaaacacggt gtccttctgg aagacgtagc
     7021 ccacctgggg tggcggggcg ccggtgaccc gcaggccgtc caccacgatc tcgccgcccg
     7081 acggcgcgac gagcccgccg atcatgttga ggatggtgga cttcccgcac ccggacggcc
     7141 ccacgatggc cacgaactcg cgctcgtgga cgtcgaagga caccggagag acggcgggga
     7201 cctctcgagg accctggccg aaggtcttcg agaggtcccg gatcgagacg cgagcgcgcg
     7261 gcgccactgc cgccggctta cttgtaccgg gcctgcgcct tcttgacgaa gctcatgtcc
     7321 accgcctgct tgtacgggat cggcttgtcg accgcggtgg gcacccacac cttcatgccg
     7381 cgctcgtagt ctcgctcctc gatcacgaag tcccagtcga agatggtccg gtagtagcgg
     7441 accgactcga gcaccacctc gcgcttgaag gcgtccatgt agggcttcca gagcaggtcg
     7501 gtcacctcgt cgtccttcgc cttccggatc cactgctggg cgcggtagtt cgcgttgacg
     7561 taggcctgca cgaggtcggg cgacttctcg atggtctcct tgagcacgta cccgaccgtg
     7621 accgggatcg gcccgccgaa cacccggttc cacgccttct cgtcccggat gtcgtagatc
     7681 ggcttgccga agtcctcctc gaccgcggcg gcctgccact cgggcaccgc catgatcgcg
     7741 tcgaacttgc ccgccttgag cccgcccagc atggtgctgg acgccccgcc gccgacccac
     7801 tcgacgtggt cgttgaccgg gcgcccgtcc gcccccttga tgttctggag cacgtagacg
     7861 ccgtagacgt aggtgccgga gccaatggcg gtcgcggcca ccaccgcctt gcgccccacc
     7921 agcttgccgt cggcgagggc ctccaccgtc ttgaccccct tgtcccacag ctccttgcgc
     7981 accacgatgt tcgcgtagga gcagcgggtg tcggtggcga ag
//
LOCUS       DATMCV010000300         8237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_548602, whole genome shotgun sequence.
ACCESSION   DATMCV010000300 DATMCV010000000
VERSION     DATMCV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548602"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VNU03_06960"
     CDS             complement(<1..139)
                     /locus_tag="VNU03_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HWP74235.1"
                     /translation="MDGVHDLGGMHGFGPVEREENEPVFHAAWEAAMLAIQRAGGARG
                     YY"
     gene            complement(186..770)
                     /locus_tag="VNU03_06965"
     CDS             complement(186..770)
                     /locus_tag="VNU03_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74236.1"
                     /translation="MSTDAMTVQEHLDGSRTEYFAVECTEAALLALLREVFEEHWEHV
                     IFGPCIEGAVFEGRFAARPRLTLLDGYATVQLQGSESWHFHLCIGLHRGSPGLPTPPE
                     LAEWRRCRRAAFFQSRDPAGRPSAWGLRLWNGRDEQMLTVFLPNPWIDPARGGRYVDT
                     PDWSRLRLWMDLRARHAGVPAEPPPADLTPPRTH"
     gene            complement(760..1383)
                     /locus_tag="VNU03_06970"
     CDS             complement(760..1383)
                     /locus_tag="VNU03_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74237.1"
                     /translation="MAALGSDPGFTPRELRALQALGSPVGVQRALDAMPYHLAGTAWS
                     PRRVLRERTAHCLEGAVFAAAALRVLGFPPLLLDLEAVQDTDHVVAVFQVRGHWGAIA
                     KSNFSGLRYREPVHRNLRELVMSYFDGYINLRGERTLRAYSRPVNLARFDRRRPGWAT
                     GEGDLWWIAEHLLEIRHTRLLDPAMVRALSRVDRRALAAGMVGYREH"
     gene            complement(1402..2682)
                     /locus_tag="VNU03_06975"
     CDS             complement(1402..2682)
                     /locus_tag="VNU03_06975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP74238.1"
                     /translation="MTSSPRDADVLVIGAGPGGTTTATFLAQGGLRVALVEREAFPRF
                     KVGESLIPTCMDICKRLGVLDRVMGHGFQVKYGATFHDQELDLETTFDFQPGRPWPSF
                     TLDVHRAEFDQILLEHAVSHPGVTLHQPATVEKVAFDADGVTARLSDQEGERELRASF
                     VVDASGRDAFLAARQGQRKPRPGLGKVAIFAYFRGARRFPGRSEGNVRLYIFPEGWFW
                     YIPLARDETSVGCVLHQRVVKARRGTLQELFDEMVERCVKISENLKGSERVTELYTTS
                     NFAYTIEPITGDRFLCVGDAVAFVDPIFSPGVFLAMQTGELAAGAILRAFRTGRFEAR
                     RFRAYERAVRRGTKPFERFIESYYDRAFLEVFMRPKNVLGSVPAVTGVLAGGSFGLLP
                     KHLHASLWFFHQVVRLTRWANLRRGVILESRLDW"
     gene            2811..3245
                     /locus_tag="VNU03_06980"
     CDS             2811..3245
                     /locus_tag="VNU03_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP74239.1"
                     /translation="MSEIRIESDPHASESLKQAVTDHLDMYNVGVTGFTEYSPVNLFL
                     RDGGDEVQGGLLAAIWGGVMYVRILWVARALRGKGHGQRLLAAAEQRAIERGCRHVFL
                     DTFNFQAPEFYVKQGYEIYARADDWPVGHAHFFLRKALPSPR"
     gene            3242..3619
                     /locus_tag="VNU03_06985"
     CDS             3242..3619
                     /locus_tag="VNU03_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HWP74240.1"
                     /translation="MIKLVFCLRRLPHLSREDFQRYWLESHGPLVRELAPHLRVKRYV
                     QVHTVTSAFSEAMSRHRGAPDDFDGVAELWWDSVDDFARAGTTKEGRDAGRRLLEDEK
                     RFIDLSRSPIWFGEEHALVDLTR"
     gene            3760..4431
                     /locus_tag="VNU03_06990"
     CDS             3760..4431
                     /locus_tag="VNU03_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWP74241.1"
                     /translation="MRKKALLLSSLALLAVSAAAAPRAEAISCINVVNVTLLPAAGCD
                     FGGLTFSNFNVSASAGFSAATVGTGAASTATSSNVNLIYQLSSTPGVGPGDVLLSYAV
                     TASPGIKLTGIDLTNDAIMGPVVIGEVACRVMFSPTGTCAPADRLAALVATTGQTMNA
                     FFASPVDFVFLHKDIDIGVGGFISDFSNSHDISPTPEPATLLLLGSTLTGMGLAWRRR
                     GGHLG"
     gene            4969..5655
                     /locus_tag="VNU03_06995"
     CDS             4969..5655
                     /locus_tag="VNU03_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWP74242.1"
                     /translation="MGRFAIFALVALIVLMGSVALTPSAEAVPSCMNAGNVTGLGSIG
                     CDLGSLNFSNFVVSPVGVAANIFLGGLSAVVANNVNLTFQVSHDPSPASLADILFYYT
                     VKTLSGQTELGGVDLYNPGKNVTIRETICATNFLNGVCTSGSLADYVVTGGSIGNATF
                     TPLESTIYVRKDIQLLQNSFISEFTNSHDEPPAEAPEPATLLLLGSSLTALGVAARRR
                     RARRNTGLAA"
     gene            complement(5720..7615)
                     /locus_tag="VNU03_07000"
     CDS             complement(5720..7615)
                     /locus_tag="VNU03_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HWP74243.1"
                     /translation="MATIYDEVYRRAQRDPEGFWAAAAEDIHWERRWDRVLDASRPPY
                     YRWFAGGRLNTCYNALDVHVDRGRGKQIALIYDSPVTGTVRSFTYFELRDEVARFAGA
                     LRRQGVEPGDRVILYMPMVPEAVIAMLACARIGAVHSVVFGGFASKELATRIDDAKPT
                     VMLSASCGIEGSRVIPYKPLLDQAIEMASHKPRRCLILQRPMEPARMVPGRDVDWQEA
                     TAAASPAECVPVEATDPLYILYTSGTTGIPKGVVRDNGGHAVALKWSMGAVYGMGAGE
                     VYWAASDIGWVVGHSYIVYAPLLKGCTTILYEGKPVGTPDPGAFWRVCAEHGVSALFT
                     APTAFRAIKKEDPKGEHMARYDLSRFRTLFLAGERCDPDTLIWARERLEVPVVDHWWQ
                     TETGWPMAANCVGLGMLPVKPGSPTKIVPGYDIRVLGEDNQEMPAGQIGSIAVKLPMP
                     PGSLPTLWNNDAGYERSYLTRHPGFYLTGDAGYKDEDDYIYIMSRVDDIINVAGHRLS
                     TGAMEEVLSSHPDVAECAVMGVADEIKGEVPVGLVVTKAGVARADADLCRELVELVRE
                     RIGPVAAFKTVAVVKRLPKTRSGKILRGTMKKIADGVEHGVPATIDDPTVLDEIGDSL
                     KALGYPR"
     gene            7720..>8237
                     /locus_tag="VNU03_07005"
     CDS             7720..>8237
                     /locus_tag="VNU03_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP74244.1"
                     /translation="MTRRRRLAALGLALLLGGGLSASAHAADEEVRTIPARPGVTESF
                     LLMRPDGAPVATVVIFAGGDGNLALTRAGLGQLQGNFLVRTRPRWVREGLMVAILDTP
                     SDHPRGLWNFRTTKEHAADVKQAIAAVREIAPAPVWLVGTSMGTLSAANAAARLTEGG
                     PDGIVLTSSVTGT"
BASE COUNT         1252 a         2965 c         2759 g         1261 t
ORIGIN      
        1 tgtagtagcc gcgcgcgccg cccgcgcgct ggatcgcgag catcgccgct tcccacgccg
       61 cgtggaagac gggctcgttc tcctcccgct ccaccggccc gaagccgtgc atgccgccca
      121 ggtcgtgcac gccgtccatg cccgcgattc tatcggctgg ggtcaggtct tacaatccga
      181 cattgtcagt gggttcgcgg cggcgtgagg tcggccggcg ggggctcggc gggaacgccc
      241 gcgtggcggg cgcgcaggtc catccagagg cggaggcggg accagtcggg cgtgtccacg
      301 tagcggccgc cgcgggcggg gtcgatccac gggttcggca ggaagacggt cagcatctgc
      361 tcgtcacgcc cgttccacag gcgcaggccc cacgcgctcg gccgaccggc cggatcgcgg
      421 ctctggaaga acgcggcccg ccggcagcgg cgccactccg ccagctcggg cggcgtgggc
      481 aggccaggcg agccccggtg gaggccgatg cagaggtgga agtgccagga ctcgctgccc
      541 tggagctgca ccgtggcgta gccgtcgagc agcgtcaggc gcggccgcgc cgcgaagcgc
      601 ccctcgaaca ccgcgccctc gatgcacggg ccgaagatga cgtgctccca gtgctcctcg
      661 aagacctcgc gcagcagcgc gagcagcgcg gcctccgtgc actcgaccgc gaagtactcg
      721 gtgcggctgc cgtccaggtg ctcctgcacg gtcatcgcgt cagtgctcac ggtagcccac
      781 catcccggcc gcgagcgcgc gtcggtccac gcgcgagagc gcgcgaacca tcgcggggtc
      841 gaggagccgg gtgtgccgga tctcgagcag gtgctccgcg atccaccaga ggtctccctc
      901 cccggtcgcc caccccggcc gccgccggtc gaagcgggcc aggttcaccg gacgcgagta
      961 ggcccgcagg gtgcgctcgc cccgcaggtt gatgtagccg tcgaagtagc tcatgaccag
     1021 ctcccgcagg ttccggtgca ccggctcgcg atagcgcagc ccggagaagt tggacttggc
     1081 gatggcgccc cagtgcccgc gcacctggaa caccgccacc acgtggtcgg tatcctgcac
     1141 cgcctccagg tcgagcagca gcggcgggaa gccgagcacc cggagcgccg ccgccgcgaa
     1201 gacagcgccc tcgaggcagt gggcggtccg ctcgcgcagc acgcggcgcg gcgaccacgc
     1261 ggtcccggcc aggtggtagg gcatcgcgtc gagcgcgcgc tggacgccca ccgggcttcc
     1321 gagggcctgc agcgcgcgca gctcgcgcgg ggtgaagccc ggatccgacc cgagggcggc
     1381 cacgccgggc gggtcggcta gctaccagtc gaggcgggac tcgagaatga ccccgcgtcg
     1441 caggttcgcc cagcgggtga gccgcaccac ctggtggaag aaccacagcg aggcgtgcag
     1501 gtgcttcggc agcaggccga acgatccgcc cgcgagcacc ccagtcaccg cggggaccga
     1561 cccgagcacg ttcttcggtc gcatgaagac ctcgaggaag gcgcggtcgt agtacgactc
     1621 gatgaagcgc tcgaacggct tggtgccccg gcgcaccgcc cgctcgtacg cgcggaaccg
     1681 ccgggcctcg aaccggccgg tgcggaacgc gcgcaggatc gcccccgcgg ccagctcgcc
     1741 ggtctgcatc gccaggaaca cgccgggcga gaagatcgga tccacgaagg ccaccgcgtc
     1801 gcccacgcag aggaaccggt cgccggtgat cggctcgatg gtgtaggcga agttggacgt
     1861 ggtgtagagc tcggtgaccc gctcggagcc cttcaggttc tccgagatct tcacgcagcg
     1921 ctccaccatc tcgtcgaaca gctcctgcag cgtcccccgg cgggccttca ccacgcgctg
     1981 gtggagcacg cagcccacgc tggtctcgtc gcgcgcgagc gggatgtacc agaaccagcc
     2041 ctccgggaag atgtagaggc ggacgttgcc ctccgaccgg cccgggaagc gccgcgcccc
     2101 gcggaagtag gcgaagatcg ccaccttgcc caggcccggc cgcggcttgc gctgcccctg
     2161 gcgggcggcc aggaacgcgt cgcgcccgct cgcgtccacc acgaacgagg cgcgcagctc
     2221 gcgctcgcct tcctggtcgc tgaggcgcgc ggtcaccccg tcggcgtcga aggccacctt
     2281 ctccactgtg gccggctggt ggagggtcac gccgggatgg gacaccgcgt gctcgaggag
     2341 gatctggtcg aactcggcgc ggtgcacgtc gagcgtgaac gagggccacg gccgcccggg
     2401 ctggaagtcg aaggtggtct cgagatcgag ctcctggtcg tggaaggtcg ccccgtactt
     2461 cacctggaag ccgtgaccca tcacccggtc gaggaccccg agccgcttgc agatgtccat
     2521 gcaagtcggg atgagcgact cgccgacctt gaagcgcggg aaggcctcgc gctcgaccag
     2581 ggccacgcgc aggccaccct gggccaggaa ggtcgcggtc gtggttccgc ccggcccggc
     2641 gccgatcacc agcacgtcgg cgtcacgcgg agagctcgtc acccccggca tcttacccga
     2701 taggccgccg tcgggcgagt ctgccgaagg ccccggattt gcgaccttcc ttggcccgtg
     2761 ctagcctgcg cccggccaag ccccggcgcc ggcaggaggc cacgacgacc atgagcgaga
     2821 tccggatcga gtccgacccc cacgcatccg agagcctcaa gcaggcggtc accgaccacc
     2881 tcgacatgta caacgtgggc gtgaccgggt tcaccgagta ctccccggtc aacctcttcc
     2941 tccgcgatgg cggcgacgag gtccagggcg gcctcctggc cgcgatctgg ggcggcgtca
     3001 tgtacgtccg gatcctctgg gtcgcccggg ccctgcgggg caagggccac gggcagcggc
     3061 tgctggccgc ggcggagcag cgcgcgatcg agcggggctg ccgccacgtc ttcctcgaca
     3121 cgttcaattt ccaggccccc gagttctacg tcaagcaggg ctacgagatc tacgcccgcg
     3181 cggacgactg gccggtgggg cacgcccact tcttcctccg gaaagctctc ccgagcccga
     3241 gatgatcaag ctggtgttct gcctgcgccg cctccctcac ctctcgcggg aggatttcca
     3301 gcgttactgg ctcgagtcgc acggcccact ggtgcgcgag ctcgcgcccc atctgcgggt
     3361 gaagcgctac gtgcaggtgc acaccgtgac gtccgccttc agcgaggcca tgagccgcca
     3421 ccggggcgcc ccggacgatt tcgacggcgt cgccgagctg tggtgggact cggtggacga
     3481 cttcgcccgt gcgggcacca cgaaggaagg ccgcgacgcg gggcgtcgcc tgctcgagga
     3541 cgagaagcgc ttcatcgacc tgtcgcgctc tccgatctgg ttcggcgagg agcacgccct
     3601 ggtcgatctt acgcggtagc ttttcgtcgc tactcagtca aagattctcc ccttcacgct
     3661 aactcgtcga tatctcgtcg cccatgttct ggcatgtcag ttgctcgacg acggcagccc
     3721 cccgttgatg tcttggagag ggggtgaagg aggtttccaa tgcggaagaa agctcttctc
     3781 ctctcttctc tggccctcct cgcggtcagt gcggccgccg caccgcgagc cgaggcgatc
     3841 agctgcatca acgtggtgaa cgtcacgctc ctgccggccg ccggatgcga ctttggcggc
     3901 ctcacgttct ccaacttcaa cgtcagcgcg agcgccggat tcagcgccgc cacggtggga
     3961 acgggtgccg cttccaccgc cacgtccagc aacgtcaacc tgatctatca gctgtcctcg
     4021 accccgggcg tgggaccggg cgacgtgctg ctcagctacg cggtgacggc ttcaccggga
     4081 atcaagctga ccggcatcga tctcacgaat gacgccatca tgggcccggt cgtgatcggc
     4141 gaagtggcgt gccgcgtgat gttctccccg acgggcacct gcgcgcccgc cgaccggctg
     4201 gccgcgctgg tagccaccac gggtcagacc atgaacgcct tcttcgccag ccccgtggat
     4261 ttcgtgttcc tgcataaaga catcgacatc ggcgtcggag gcttcatcag cgacttcagc
     4321 aacagccacg acatttcccc gaccccggag ccggccacgc tcctgctgct cggcagcacg
     4381 ttgaccggga tgggactggc gtggcggcgc cggggaggcc acctcgggta aatgtcaccc
     4441 agaccggtgt cacccacgag cgtcgagcct cctcggcgct cgtgcggtgt tcacttcccg
     4501 agccataaaa cttctgagaa cgcccggccc cgtgtagcct catcggtatg tcgcctgtcg
     4561 atctcgatct gagccccgat ctggcctgtc tggtcgaccg cgtccgtcag aaccggcagc
     4621 cctgggtggt cgtcgcgacg gcggccctcg aggcatggga gaagcgagat cccgcgggtt
     4681 gggagcgggt atccgagtgg ctcgccgaga atggggtgac cgtcgtgcga atctgaagct
     4741 cccggactta cgcggtaact ttcgggaaaa ctgtatccct tacacagtaa ctagtaccta
     4801 ccggggtccc ggccccaaca ggatgtagtc tcggggcaac cgaactcgct gcattggtgg
     4861 gcgtttcgcc acccggcctc agacgcgcga tttgcctccc accgacgggc gcgtggcacg
     4921 caacttgtct ccctctgacc catcttcaac tttccaagga gggttcagat gggtcggttc
     4981 gccatattcg ctctggttgc gctcatcgtg ctgatgggca gcgtcgcgct cactccttcc
     5041 gccgaggccg ttccctcgtg catgaacgcc ggcaacgtga cgggcctggg ctcgatcggc
     5101 tgcgatctcg gctccctgaa cttctccaac ttcgtggtga gcccggtggg cgtggccgcg
     5161 aacatttttc tcggcggcct ctccgccgtc gtcgccaaca acgtcaacct gaccttccag
     5221 gtgtcgcacg atccctcgcc cgcgagcctg gccgacatcc tgttctacta cacggtcaag
     5281 accctcagcg ggcagaccga gctgggcggg gtggatctct acaacccggg caagaacgtc
     5341 acgatccgcg agacgatctg cgccaccaac ttcctgaatg gagtgtgcac gagcggctcg
     5401 ctcgcggact acgtcgtgac cggtggaagc atcggcaatg cgaccttcac cccgctcgag
     5461 tcgacgatct acgtgcgaaa ggacatccag ctcctgcaga actccttcat cagcgagttc
     5521 accaacagcc acgacgagcc cccggccgaa gcgccggagc cggccaccct gctgctcctc
     5581 ggtagcagcc tgaccgcgct gggcgtggcc gcccgccgcc ggcgggcccg ccgcaacacc
     5641 ggcctcgccg cctagtcccg gggtcaggtc ttacattccg acaatgtagg aatgtaagac
     5701 ctgaccccag cccgaggcgc tatcgcgggt agccgagagc cttgagggag tcgccgatct
     5761 cgtcgaggac ggtggggtcg tcgatggtgg cgggcacgcc gtgctcgaca ccgtcggcga
     5821 tcttcttcat ggtcccgcgg aggatcttgc ccgagcgcgt cttgggcagc cgcttcacga
     5881 ccgccaccgt cttgaacgcg gccaccggcc cgatccgctc gcgcaccagc tcgaccagct
     5941 cgcggcagag atctgcgtcc gcgcgcgcga cgccggcctt ggtgaccacg agacccaccg
     6001 ggacctcgcc cttgatctcg tcggccaccc ccatcaccgc gcactccgcc acgtccggat
     6061 gcgaggagag cacctcctcc atggcgccgg tggagagccg gtgcccggcc acgttgatga
     6121 tgtcgtccac ccggctcatg atgtagatgt agtcgtcctc gtccttgtag cccgcgtccc
     6181 cggtgaggta gaagccggga tgccgcgtga ggtaggagcg ctcgtacccc gcgtcgttgt
     6241 tccacagcgt gggcaggctc ccgggcggca tcggcagctt caccgcgatc gacccgatct
     6301 ggcccgccgg catctcctgg ttgtcctcgc ccagcacgcg gatgtcgtag cccggcacga
     6361 tcttggtcgg cgagcccggc ttcaccggga gcatgccgag ccccacgcag ttcgcggcca
     6421 tcggccagcc ggtctcggtc tgccaccagt ggtcgaccac cggcacttcg agccgctcgc
     6481 gcgcccagat cagggtgtcg gggtcgcagc gctcgccggc gaggaagagg gtgcgaaagc
     6541 gcgagagatc atatcgcgcc atgtgctcac ccttcgggtc ttccttcttg atcgcccgga
     6601 acgcggtggg cgcggtgaac agggcgctca ccccgtgctc ggcgcagacc cgccagaacg
     6661 cgcccggatc cggggtgccg accggcttgc cctcgtagag gatcgtcgtg cagcccttga
     6721 gcagcggcgc gtacacgatg taggagtggc ccaccaccca gccgatgtcg gaggcggccc
     6781 agtacacctc ccccgccccc atgccgtaca ccgcgcccat gctccacttg agcgcgaccg
     6841 cgtggccgcc gttgtcgcgc accacgccct tggggatccc ggtggtgccc gaggtgtaga
     6901 ggatgtagag cgggtcggtg gcctcgaccg gcacgcactc cgcgggagac gcggccgcgg
     6961 tcgcctcctg ccagtcgacg tcgcgcccgg gcaccatgcg cgccggctcc atcggccgct
     7021 gcaggatcag gcagcggcgc ggcttgtgcg aggccatctc gatcgcctga tcgagcagcg
     7081 gcttgtaggg aatgacccgg ctaccctcga tcccgcagga ggcggagagc atcacggtgg
     7141 gcttcgcgtc gtcgatgcgg gtggccagct ccttggacgc gaagccgccg aacaccaccg
     7201 agtggaccgc cccgatacgc gcgcaggcga gcatcgcgat caccgcctcc ggcaccatgg
     7261 gcatgtagag gatgacgcga tcgcccggct cgacgccctg ccgccgcagc gcgcccgcga
     7321 accgggccac ctcgtcgcgc agctcgaagt aggtgaagga ccgcacggtg ccggtcaccg
     7381 gcgaatcgta gatcagcgcg atctgcttgc cgcgaccgcg gtccacgtgc acgtcgaggg
     7441 cgttgtagca ggtgttgagc cgaccgcccg cgaaccagcg atagtagggc gggcggctcg
     7501 cgtcgagcac gcggtcccac cgccgctccc agtggatgtc ctcggccgcc gcggcccaga
     7561 acccctccgg gtcccgctgg gcgcgccggt agacctcatc gtagatggtc gccatcctca
     7621 ccgcctcctc gggcgtcgac tcctgacgcg accggactgt acaccgtcgc cccgatgcct
     7681 tgccagcgga accggccacg ccgtatcatc gacgcgatca tgacgcgccg tcgccggctg
     7741 gccgctctcg ggctcgcgct gctcctgggg ggcggcctct ccgcctccgc tcacgccgcc
     7801 gacgaggagg tgcgcacgat cccggcccgt cccggcgtca ccgagtcgtt cctgctgatg
     7861 cgcccggacg gtgccccggt cgccaccgtg gtcatcttcg cgggcggcga cggcaacctg
     7921 gccctgacgc gcgccgggct cggtcagctc cagggcaatt tcctggtgcg gacccgcccg
     7981 cgctgggtcc gcgagggcct catggtggcg atcctcgaca cgccctccga ccacccgcgc
     8041 gggctgtgga acttccgcac caccaaggag cacgcggccg acgtgaagca ggcgatcgcg
     8101 gcggtgcgcg agatagcccc ggcgccggta tggctggtgg gtacgagcat gggcacgctc
     8161 tccgcggcca acgcggcggc ccggctcacc gagggcggac ccgacggtat cgtgctcacg
     8221 tcctcggtga ccgggac
//
LOCUS       DATMCV010000301         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_418079, whole genome shotgun sequence.
ACCESSION   DATMCV010000301 DATMCV010000000
VERSION     DATMCV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418079"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1765)
                     /locus_tag="VNU03_07010"
     CDS             complement(95..1765)
                     /locus_tag="VNU03_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74245.1"
                     /translation="MIAAAIAVLVFVLYIATYASIPAGDGYWVLNNVDRVDLELLFNP
                     PSLLTQLGFLGLRRLADLLGLQIATLTIIQTVNAMAAGVGAALLYGIVRTLGGSRLLG
                     GLTAGLLAVSFGYWYFADGEAQLLSLVVLLAIVWLITRARARGDWTWRFVAGVALLNS
                     LAGLLRQENVIFGFAAVAFLAVGRPWRRALSDALVYAAAGSLGTWTAVLFIGLGWAPG
                     VHTVADAVRWYLWVFRVHVGAVQDFQGFEHATKFDVPRLVKGQMTAFIAGTQAVVDSM
                     RDRALLGRAYVLGLIALTVAAYAMMVTLAVELRRLRRLVETRLAAMAVASAVWIVAYK
                     ALVHGWLWPTVTKYQVVTVPPLIILCVLGVIAAQKAGDGARARRLTGLVAALVALVFV
                     VDLSGGILPWRHYGQMKAALEVRRSRDFRAEDLFISSESGIDPIFARLYQGGDAHHVG
                     VKDVFVQKPTREAFASIRAAIDGQLALGRRVFVYNLVPGPYSLVGMNQAPTRAGVPLG
                     ARDFEIFFDELRATYALRPVFSYWEESKAPLYLFGERQEPFFEVGARS"
     gene            complement(1762..2355)
                     /locus_tag="VNU03_07015"
     CDS             complement(1762..2355)
                     /locus_tag="VNU03_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74246.1"
                     /translation="MRHRFTVKYEIDCPVAIAVAVYLDAEHYVFLHKKYSDTYEVLSY
                     EPETRKIFIRESWELFGLRIGQTCMTEYVPPAEFLNYDIEPYPRWLPSVHHVMKTRTR
                     VIYTEIPERNSTWSRLEIDLELPFWLWPFRRFIERAITRLKVEKDEQDIDMIRRRAAL
                     FGRENNSIYLADHQFILHKDEYLRHFGPNSKPLTPLA"
BASE COUNT          421 a          805 c          878 g          333 t
ORIGIN      
        1 aacagatcgc cgagcaccgc gttgttcagg accccgagcc ggttggcgac cccgtaggcg
       61 gagtggaggc cttcgaccac gcgcccgcgg aggctcacga tcgcgccccg acctcgaaga
      121 acggctcctg ccgctcgccg aagagataga ggggggcctt gctctcctcc cagtagctga
      181 acactggccg cagggcgtag gtcgcccgca gctcgtcgaa gaagatctcg aaatcccgcg
      241 cgccgagcgg cacgcccgcc cgggtggggg cctggttcat gccgaccagc gagtacggcc
      301 cggggacgag gttgtacacg aagacgcggc gcccgagcgc gagctggccg tcgatggcgg
      361 cgcggatcga cgcgaacgcc tcccgcgtcg gcttctggac gaagacgtcc ttgacgccga
      421 cgtggtgcgc gtcgcctccc tggtagagac gcgcgaagat cgggtcgatg ccggactcgg
      481 acgagatgaa gagatcctcc gcgcggaagt cgcggctgcg ccggacctcg agggcggcct
      541 tcatctgccc gtagtggcgc cacgggagga tcccgccgct gagatcgacg acgaagacca
      601 gcgcgaccag cgccgcgacg agcccggtga gccggcgggc ccgcgcgccg tccccggcct
      661 tctgcgccgc gatcactccg agcacgcaca ggatgatgag cggcgggacg gtgaccacct
      721 ggtacttggt cacggtcggc cagagccacc catggaccag ggccttgtag gccacgatcc
      781 agaccgcgga ggcgaccgcc atcgcggcga gccgggtctc gacgagacgg cggagccgtc
      841 gcagctcgac cgcgagggtc accatcatcg cgtaggccgc gacggtgagc gcgatgaggc
      901 cgaggacgta ggcccggccc agtagcgccc ggtcgcgcat cgagtcgacg accgcctggg
      961 tgcccgcgat gaacgcggtc atctggccct tcaccagccg cggcacgtcg aacttggtgg
     1021 cgtgctcgaa gccctggaag tcctgcaccg cgcccacgtg cacgcggaag acccacaggt
     1081 accagcgcac cgcgtcggcg acggtgtgga ctccgggggc ccatccgagc ccgatgaaga
     1141 gcaccgcggt ccacgttccg agcgatccgg ccgccgcgta cacgagcgcg tcgctcaggg
     1201 cccgccgcca cgggcgaccc accgcgagga aagcgacggc ggcgaacccg aatatgacgt
     1261 tctcctgcct caacaggccg gcgagcgagt tcagcagcgc gacccccgcg acgaagcgcc
     1321 aggtccagtc gccgcgggcg cgagcccgcg tgatcagcca gacgatcgcc aggaggacga
     1381 cgagcgagag gagctgcgcc tcgccgtccg cgaagtacca gtagccgaac gagaccgcga
     1441 gtaggccggc ggtcagcccc ccgaggagcc gcgagccgcc gagggtccgg acgatcccgt
     1501 agagcagggc cgcgccgacc ccggcggcca tcgcgttcac ggtctggatg atcgtgaggg
     1561 tcgcgatctg gagcccgagc aggtccgcga gccgtcggag cccgaggaag ccgagctggg
     1621 tcagcagcga ggggggattg aagagcagct cgagatcgac gcggtcgacg ttgttcagga
     1681 cccagtagcc gtcgccggcc gggatcgagg cgtaggtggc aatgtagagg acgaagacca
     1741 gcaccgcgat cgcggcggcg atcacgcgag gggcgtgagc ggcttcgagt tgggtccgaa
     1801 gtgccggagg tactcgtcct tgtggaggat gaactggtgg tcggcgaggt agatcgagtt
     1861 gttctcgcgg ccgaacagcg cggcgcgccg tcggatcatg tcgatgtcct gctcgtcctt
     1921 ctcgaccttc agccgggtga tcgccctctc gatgaacctg cggaacggcc agagccagaa
     1981 cggcagctcc aggtcgatct cgagccggct ccacgtcgag ttccgctcgg ggatctcggt
     2041 gtagatgaca cgggtcctcg tcttcatgac gtggtggacg ctcggcagcc accggggata
     2101 gggctcgatg tcgtagttga ggaactcggc gggcgggacg tactcggtca tgcaggtctg
     2161 gccgatgcgc agcccgaaca gctcccacga ctcccggatg aagatcttgc gggtctccgg
     2221 ctcgtaggag agcacctcgt aggtgtcgga gtacttcttg tggaggaaga cgtagtgctc
     2281 cgcgtcgaga tagaccgcga ccgcgatggc gaccggacag tcgatctcgt atttgaccgt
     2341 gaagcggtgc ctcacggctc gctccgccgg cgccgcacgt aagcgcggtc gccgaccatc
     2401 tggtagtcca ggggagcgag cgcgtaccgg ggcgcct
//
LOCUS       DATMCV010000302         6428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_750746, whole genome shotgun sequence.
ACCESSION   DATMCV010000302 DATMCV010000000
VERSION     DATMCV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750746"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1266
                     /locus_tag="VNU03_07020"
     CDS             106..1266
                     /locus_tag="VNU03_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWP74247.1"
                     /translation="MQRSEERILTTHVGSLPRPPALRDLLVRQDRGEAVDAAALEREV
                     GAALSRVVTGQLEAGIDVGNNGEQPRAGFSTYVASRMKGFGGTSRRELAQDLIDFPDY
                     AEMLARRRRDAARIGDAPQAIAEIDYADLGPAERECDAFLRETDARPRRFAERFMTAA
                     SPGVIATILLSAHYASHEQYVMALSREMRKEYELIHRRGLLLQVDCPDLAMERSRFFR
                     RDSLERFLQMVELHVEAINRATASIPPERIRLHLCWGNYDGPHTHDVPLEAVLPIVAR
                     ARVGALSLPLANPRHQHEYRVLRQHRLPDAMLLLPGVIDTTTNYVEHPEVVADRICAA
                     VEAVGDRTRVIASTDCGFGTFAGSEMVAHGVVWAKLGALTEGAAIASRRLWS"
     gene            1314..2018
                     /locus_tag="VNU03_07025"
     CDS             1314..2018
                     /locus_tag="VNU03_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWP74248.1"
                     /translation="MNSERFAKGMTFDEYVKFIGSPENLAREGFDIRIFSLVRPRVDW
                     SAYLRDRFARARLSAEQTAAIQWLAAQPGGPAKIAVISEEWSSDCRRDVPYLARLAQA
                     GGLELRIFPRDGDAMLMKGLPEPTVGNGDLVCEYANDKNGQRWASVPVAVFFTADFSE
                     IYRYVEYPAIYRKERVIGHLRAARADETADQTKARSGKEITALLESPFPDVWAQAGIA
                     EILSALHERILTGARG"
     gene            complement(2043..2465)
                     /locus_tag="VNU03_07030"
     CDS             complement(2043..2465)
                     /locus_tag="VNU03_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74249.1"
                     /translation="MDLMLSTHDTGYERPDGPTIAKVLASLDGGRNVLATLGTSDSSY
                     IQASGSVQAGFGLDLQEGSIERRFRTRDRALPLAWVTEVFQRYAQADLGWRDRVEWEQ
                     ERIVVPRETWMNSWAAYIALMIAVAGAIWLWHTWRAVP"
     gene            complement(2481..3644)
                     /locus_tag="VNU03_07035"
     CDS             complement(2481..3644)
                     /locus_tag="VNU03_07035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP74250.1"
                     /translation="MDFALTESQEVIKKEVGALARTFSLDYWLEKDDKAEYPSDFVKA
                     FADNGWLGITIPEEYGGAGLGVTEAALMLHEICASGAGTSGCSPIHFYVFPPTPLVKY
                     GTEAMKRKYLPGIVSGETVMAFGVTEPNAGTDTSRITTRAEKKGDRWVVNGRKVWTTN
                     AQHANRILLLARTSDRDPAKPLKGMTLFFADFNREAITVRLIHKLGRAAVDSNEIFID
                     NLEIPEEDVVGAVGEGFYHLLDSLNPERIVIGIEAVGIGRAALDRAVQYAKERVVFDR
                     PIGKNQAIAHPLAHAMAQLDAAEMMCLKAAWLFDNGKPCGREANAAKMLAAEAGFNAC
                     DAALQTFGGYGYAKEFHVERLWREIRLYKIAPVSQQMALNYLAEHVLGLPRSY"
     gene            3728..4459
                     /locus_tag="VNU03_07040"
     CDS             3728..4459
                     /locus_tag="VNU03_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWP74251.1"
                     /translation="MDETTPAIQDYLAAIYDLAGTDKPVIGARLARHMHISAPSITEA
                     IRRMQKDGYIRIGGRKEIRLTTKGLGIAETMARRHRLIERWLTDELGLDWSRAHDEAH
                     RLEHSLSPVVEERLAEKLGMPSTCPHGNPIPGMPAPEAHRPIPLNQAPAGHPFVVERI
                     TEEAEADRQLLRFLWESGIRPGSRIVVSEIAPYAGTVSIVLDGRTVTMGLSASNKIWV
                     YDPMEPEPAARQKGARAHRPAKSRA"
     gene            4463..5218
                     /locus_tag="VNU03_07045"
     CDS             4463..5218
                     /locus_tag="VNU03_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HWP74252.1"
                     /translation="MAVKALYALQNGYLGFERTGLFYGERSPERVAIPVTCYLIRTSD
                     ATILFDTGISPRAVPGLLRQDPLCRFSDEDLLVHRLDSIGLEPKDVDLVVISHLHFDH
                     AGGAAIFDTSELVVQKDEFAYAQYPAAFFASFYYRKNFDLPGYRWRLLDGDAELVPGV
                     TALRSDGHTPGHQSLLVQLPVAGPVILAGDCCYWQRSIDEEIPPGVVWDPTRAMHSIQ
                     RLKTLARLTGGRIFPSHDPAFWERAIKAPEAYK"
     gene            5289..>6428
                     /locus_tag="VNU03_07050"
     CDS             5289..>6428
                     /locus_tag="VNU03_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWP74253.1"
                     /translation="MGVLDGIKVLEMARVPPAEMPGMFLADMGADVLKVETPPETTED
                     AATKRRAAFAYVNRNKRSLALNMKAPEAQAIFKKLAASADVIVEGFRPGVMKRLGADY
                     ATLSAANPRLVYCSLSGFGHNGPYKDYPAHDGNYLSLAGVLNLIGEPDRKPIFPLNLV
                     ADYGGASMHGALGIMMALFARERTGRGQHVDVSYLDTSLALLAATPNMRFFWSDGMAP
                     KRGEGFLGGSYPYYAVYETKDRKYLTIGCTEPWLWENFCKAINRPDMVKFARKADQFV
                     RAANPEEVKAREEIEGIIRTRTRDEWYDFLVKADVCVGKVYEPEEVVTDPQVQARDMI
                     VEMQHPVHGTVKQFGQPIKLSDTPGTMRTAAPYSGEHTDQVLGELG"
BASE COUNT          974 a         2316 c         2205 g          933 t
ORIGIN      
        1 catcgatcac ctcagctacg agaaggctct gctcggagac tgggtgcggc gcgagctcgg
       61 ccgcgggacc cgctcgtgac gggcggcccg gtcgagggct gagccatgca gcgcagcgag
      121 gagcgcatcc tcaccaccca cgttggcagc ctcccccgcc cgcccgcgct ccgggatcta
      181 ctggtgcgcc aggaccgggg cgaggcggtg gacgcggccg cgctcgagcg cgaggtcggc
      241 gcggcgctct cgcgcgtcgt caccgggcag ctcgaggccg gcatcgacgt gggcaacaac
      301 ggcgagcagc cgcgcgcggg cttctccacc tacgtggcct cccgcatgaa gggcttcggg
      361 ggcacgagcc gccgggagct ggcccaggac ctgatcgact ttcccgacta cgcggagatg
      421 ctggcccggc ggcggcgcga cgcggcccgc atcggcgacg cgccgcaggc catcgccgag
      481 atcgactacg cggacctggg gccggccgag cgggagtgcg acgcattcct gcgcgagacc
      541 gacgcgcggc cccggcggtt cgccgagcgc ttcatgaccg cggcgtcccc cggggtgatc
      601 gccaccatcc tgctctccgc ccactacgcc tcgcacgagc agtacgtcat ggcgctcagc
      661 cgcgagatgc gcaaggagta cgagctgatc caccgccggg gcctgctgct gcaggtggac
      721 tgcccggacc tcgcgatgga gcgctcccgg ttcttccgcc gcgactcgct cgagcgcttc
      781 ctgcagatgg tcgagctgca cgtggaggcg atcaaccggg ccaccgcctc catcccgccc
      841 gagcgcatcc gcctgcacct ctgctggggc aactacgacg ggccgcatac ccacgacgtg
      901 ccgctcgagg cggtgctgcc gatcgtggcc cgggcgcggg tcggggccct ctcgctgccg
      961 ctcgccaacc cgcgccacca gcacgagtac cgcgtcctcc gtcagcatcg cctgcccgac
     1021 gcgatgctgc tcctgcccgg ggtcatcgat accaccacga actacgtcga gcaccccgag
     1081 gtggtggccg accggatctg cgccgcggtg gaggcggtgg gcgaccggac gcgcgtgatc
     1141 gcctcgaccg actgcggctt cggcaccttc gcgggctcgg agatggtggc ccacggcgtg
     1201 gtgtgggcca agctcggtgc cctcaccgag ggcgccgcca tcgcgagccg gcgcctctgg
     1261 tcctgacgcg ggacgggcgc cacaaggccg ggccactctg gtatcctccg cgcatgaact
     1321 ccgagcgctt cgccaagggc atgaccttcg acgagtacgt gaagttcatc ggctcccccg
     1381 agaacctcgc ccgggaggga ttcgacatcc ggatcttcag cctggtccgc ccgcgggtgg
     1441 actggagcgc ctacctgcgc gaccgcttcg cccgggcgcg gctcagcgcc gagcagaccg
     1501 cggccatcca gtggctggcc gcgcagccgg gcgggcccgc caagatcgcg gtgatctcgg
     1561 aggagtggtc gtcggactgc cggcgcgacg tgccgtacct ggcgcgcctg gctcaggcgg
     1621 gcgggctcga gctccgcatc ttcccgcgcg acggcgacgc gatgctgatg aaggggctcc
     1681 cggagccgac ggtgggcaac ggcgacctgg tgtgcgagta cgccaacgac aagaacggcc
     1741 agcgctgggc gtcggtcccg gtggcggtct tcttcaccgc cgacttcagc gagatctacc
     1801 gctacgtcga gtacccggcc atctaccgca aggagcgggt gatcgggcac ttgcgggccg
     1861 cgcgcgcgga cgagaccgcc gaccagacca aggcccgcag cggcaaggag atcacggccc
     1921 tgctggagtc gcccttcccc gacgtgtggg cccaggccgg gatcgcggag atcctgagcg
     1981 cgctccacga acggatcctc accggcgccc gcggctgagc gacggccggg cgcccgccgc
     2041 ggtcagggga cggcgcgcca ggtgtgccag agccagatcg ccccggcgac cgcgatcatg
     2101 agcgcgatgt aggcggccca gctgttcatc cacgtttcgc gcgggacgac gatgcgctcc
     2161 tgctcccact ccacccggtc ccgccagccc aggtccgcct gcgcgtagcg ctggaacacc
     2221 tcggtcaccc aggcgagcgg cagggcgcgg tcgcgggtgc ggaagcgccg ctcgatggag
     2281 ccctcctgca gatcgaggcc gaagccggcc tgcacgctcc cggacgcctg gatgtagctc
     2341 gagtcggagg taccgagggt ggccagcacg ttgcgcccgc cgtcgagcga cgccagcacc
     2401 ttggcgatgg tgggcccgtc gggccgctcg tagccggtat cgtgggtgct gagcatcagg
     2461 tccatgggag atccctgggg ctaatacgag cgcggcaggc cgagcacgtg ctcggcgagg
     2521 tagttgaggg ccatctgctg cgacaccggc gcgatcttgt agaggcggat ctcgcgccac
     2581 agccgctcca cgtggaactc cttggcgtac ccgtagcccc cgaaggtctg gagcgccgca
     2641 tcgcaggcgt tgaagccggc ctcggccgcg agcatcttgg ccgcgttggc ctcgcgtccg
     2701 cacggcttac cgttgtcgaa gagccacgcg gccttgagac acatcatctc ggccgcgtcg
     2761 agctgggcca tcgcgtgggc gagcggatgg gcgatcgcct ggttcttccc gatcggccgg
     2821 tcgaacacca cgcgctcctt ggcgtactgc accgcgcggt cgagggccgc gcgtccgatc
     2881 cccaccgcct cgatgccgat gacgatgcgc tccgggttga gcgagtcgag caggtggtag
     2941 aagccctccc ccaccgcgcc caccacgtcc tcctcgggga tctccaggtt gtcgatgaag
     3001 atctcgttgg agtcgaccgc ggcccgcccg agcttgtgga tcaggcgcac cgtgatcgcc
     3061 tcgcggttga agtcggcgaa gaagagggtc atgcccttga gcggcttggc cgggtcacgg
     3121 tcggacgtgc gcgcgaggag caggatccgg ttggcgtgct gggcgttggt ggtccagacc
     3181 ttccggccgt tcaccaccca gcggtcgccc ttcttctccg cccgggtggt gatgcgcgag
     3241 gtgtcggtgc ccgcgttggg ctcggtgacc ccgaacgcca tcaccgtctc cccgctcacg
     3301 atccccggca ggtacttccg cttcatcgcc tcggtgccgt acttgacgag cggggtcggc
     3361 ggaaacacgt agaagtggat cggggagcag ccggaggtgc ccgcgcccga cgcgcagatc
     3421 tcgtgcagca tcagggcggc ctcggtgacc ccgagcccgg ccccgccgta ttcctcgggg
     3481 atcgtgatgc cgagccagcc gttgtcggcg aaggccttga cgaagtcgct cggatactcg
     3541 gccttgtcgt ccttctcgag ccagtagtcg agcgagaagg tgcgggccag cgcccccacc
     3601 tctttcttga tcacctcctg cgattcggtc agcgcgaagt ccattgagcc cgcctcctgg
     3661 tcgggaaaga atatcacggc cgcccgggga cttgcccccc gtcgccccgg tcggtatgct
     3721 ggcgcccatg gacgagacca cgccggcgat ccaggactat ctcgcggcca tctacgatct
     3781 cgccggcacc gacaagccgg tgatcggcgc gcggctcgcg cgccacatgc acatctcggc
     3841 gccgtccatc accgaggcga tccgccgcat gcagaaggac ggctacatcc gcatcggcgg
     3901 ccgcaaggag atccggctga ccaccaaggg gctcgggatc gccgagacca tggcgcgccg
     3961 gcaccgcctg atcgagcgct ggctcaccga cgagctcggg ctcgactggt cgcgcgcgca
     4021 cgacgaggcc caccggctcg agcactccct gtcgccggtg gtcgaggagc ggctcgccga
     4081 gaagctcggg atgcccagca cctgcccgca tggcaacccg atcccgggca tgccggcgcc
     4141 cgaggcgcac cggccgatcc ccctcaacca ggcgcccgcc ggtcacccgt tcgtggtcga
     4201 gcgcatcacc gaggaggcgg aggccgaccg ccagctcctg cgcttcctgt gggagtcggg
     4261 gatccgcccg ggcagccgca tcgtggtgag cgagatcgca ccctacgcgg gcacggtgtc
     4321 gatcgtgctc gacggccgca ccgtgaccat gggcctctcc gcctcgaaca agatctgggt
     4381 gtacgacccg atggagcccg agcccgcggc ccgccagaag ggggcgcgcg cgcaccgtcc
     4441 cgccaagtcc cgcgcctgat ccatggcggt aaaggccctc tacgcgctgc agaacggata
     4501 tctcgggttc gagcgcacgg ggctcttcta cggcgagcgc tctcccgagc gggtggcgat
     4561 cccggtcacc tgctacctga tccggacgag cgacgcgacg atcctcttcg acaccggcat
     4621 ctccccgcgc gcggtccccg gtctgctgcg gcaggatccc ctctgccgct tcagcgacga
     4681 ggacctgctc gtgcaccggc tcgactcgat cgggctcgag cccaaggacg tggacctggt
     4741 ggtgatcagc cacctgcact tcgaccacgc gggcggtgcg gcgatcttcg acacctccga
     4801 gctggtggtg cagaaggacg agttcgccta cgcccagtac ccggccgcct tcttcgcctc
     4861 gttctactac cgcaagaact tcgacctgcc cggctaccgc tggcgcctgc tcgacggcga
     4921 cgcggagctg gtgcccggcg tgaccgcgct gcgtagcgac ggccacaccc ccggccacca
     4981 gtcgctgctg gtgcagctgc cggtggccgg cccggtcatc ctggccgggg actgctgcta
     5041 ctggcagcgg tcgatcgacg aggagatccc gcccggcgtc gtgtgggacc cgacgcgggc
     5101 gatgcactcg atccagcgcc tcaagacgct cgcgcggctc accggggggc gcatcttccc
     5161 gagccacgac ccggccttct gggagcgggc gatcaaggca cccgaggcgt acaagtaacc
     5221 ctcagcctca accacccctc accctgccct ctccccctta ggggagaggg agatcagcgg
     5281 agatcgccat gggcgtgctg gacggaatca aggtgctgga gatggcgcgg gtcccgcccg
     5341 ccgagatgcc cgggatgttc ctggccgaca tgggggccga cgtgctgaag gtcgagaccc
     5401 cacccgagac caccgaggac gcggccacca agcgccgcgc cgctttcgcc tacgtgaatc
     5461 gcaacaagcg gtcgctcgcg ctcaacatga aggcgccgga ggcgcaggcg atcttcaaga
     5521 agctggccgc ctccgccgac gtcatcgtgg agggcttccg cccgggcgtg atgaagcggc
     5581 tgggggccga ctacgccacg ctctccgccg cgaaccctcg actggtgtac tgctcgctct
     5641 ccggcttcgg gcacaacggc ccctacaagg actacccggc ccacgacggc aactatctct
     5701 ccctcgccgg ggtgctgaac ctgatcgggg agccggaccg caagccgatc ttcccgctga
     5761 acctggtggc cgactacggc ggagccagca tgcacggcgc cctcggcatc atgatggcgc
     5821 tcttcgcgcg cgagcggacc ggccgcggcc agcacgtgga cgtctcctac ctcgacacct
     5881 cgctggcgct gctcgcggcc acgcccaaca tgcgcttctt ctggagcgac ggcatggcgc
     5941 ccaagcgcgg cgagggcttc ctgggcggct cctatccgta ctacgcggtc tacgagacca
     6001 aggaccgcaa gtatctgacc atcgggtgca ccgagccctg gctgtgggag aacttctgca
     6061 aggcgatcaa ccggccggac atggtgaagt tcgcccgcaa ggcggatcag ttcgtgcgag
     6121 cggccaaccc ggaagaggtg aaggcgcgcg aggagatcga gggcatcatc cggacccgca
     6181 cccgcgacga gtggtacgac ttcctggtca aggccgacgt ctgcgtgggc aaggtctacg
     6241 agccggagga ggtcgtcacc gacccgcagg tgcaggcccg cgacatgatc gtggagatgc
     6301 agcacccggt gcacgggacc gtgaagcagt tcggccagcc catcaagctc tccgacaccc
     6361 cggggaccat gcggaccgcg gcgccctact cgggtgagca caccgatcag gtgctgggcg
     6421 agctcggc
//
LOCUS       DATMCV010000303         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_549061, whole genome shotgun sequence.
ACCESSION   DATMCV010000303 DATMCV010000000
VERSION     DATMCV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549061"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..727
                     /locus_tag="VNU03_07055"
     CDS             20..727
                     /locus_tag="VNU03_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74254.1"
                     /translation="MLDVRGLSVAYGDVRVLYDVTLRVGEREIVSLVGANGAGKTTTL
                     RAVSGLLPILDGEATFEGERLTGLPPSRVVERGIAHVPEGRQLFTNMTVGENLEMGAY
                     LPRAKAAMAQTLAWVTGLFPRLGERRAQLAGTLSGGEQQMLAIGRALMSRPRLLILDE
                     PSLGLAPIMVKAIFDAVRRINAEGTTVLLVEQNLVQSLRLSHRGYVLETGRIVLAGTG
                     EELLANPHTRKAFLGLS"
     gene            743..1996
                     /locus_tag="VNU03_07060"
     CDS             743..1996
                     /locus_tag="VNU03_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74255.1"
                     /translation="MTPTTRREFLKSSAMAAAATTVAPRFAAAQSPAAQSKEVLLAAV
                     VALTGTNAAWGQRTWNAFQLGCDLINEQGGIKSMGGAKLKYIVADTESKPEVAGSQAE
                     KVVGRGAVAITGTNQSAATIVATQIAEREGVPFVCATDVDPLITSRGFKYTFRTSPLV
                     SAYAADLLEYVKELGQKAGKPARTLAILSENSIVGQSSVEGATKVGKELGYEVVAGDT
                     YDASKTQNFASYISKYKGGNVDVVIGHNKPSDAILITRTMKELNYNPMAYGGILGGHV
                     STEYVNALGKDADNVLATTSWSPDADIAGLKELAKKYLDRFKEPLDSTSAGGFTAIAV
                     LWDALERAGSADRKKLRDALAATQLKTGEKMYMQLRGAKFTASGENERAGGLVFVVKD
                     KTWVTVAPKEFAKTQAPFPKPRWGA"
BASE COUNT          359 a          770 c          729 g          274 t
ORIGIN      
        1 ctcggcgagg cgtactccct tgcttgacgt gcgcggcctc tcggtggcct acggcgacgt
       61 gcgggtgctg tacgacgtca ccctgcgcgt cggcgagcgc gagatcgtct cgctggtggg
      121 ggccaacgga gcgggcaaga ccaccacgct gcgcgcggtc tccgggctcc tgcccatcct
      181 cgacggcgag gcgacgttcg agggcgagcg cctcaccggc ctgcctccct cgcgcgtggt
      241 ggagcggggc atcgcccacg tcccggaagg gcggcagctg ttcaccaaca tgacggtggg
      301 cgagaacctc gagatggggg cctacctgcc ccgcgccaag gccgcgatgg cgcagaccct
      361 ggcctgggtg accgggctct tccctcgcct gggcgagcgc cgcgcgcagc tcgcgggcac
      421 cctctccggc ggcgagcagc agatgctcgc catcggccgc gcgctcatgt cccgcccgcg
      481 cctgctcatc ctcgacgagc cgtcgctggg cctggccccg atcatggtga aggccatctt
      541 cgacgcggtg cgccgcatca acgcggaggg gaccaccgtg ctgctggtcg agcagaacct
      601 cgtgcagtca ctccgtctct cgcaccgtgg ctacgtactg gagacgggac ggatcgtact
      661 cgcgggcacg ggcgaggagc tcctcgccaa cccgcacacc cgcaaggcat tcctgggact
      721 ctcctagagg aggacacgaa ccatgacccc gaccacccgc cgcgaattcc tcaagtcgtc
      781 cgcgatggcg gccgccgcca ccaccgtcgc gccccgcttc gccgccgccc agtcccccgc
      841 cgcccagtcc aaggaggtcc tgctcgccgc ggtcgtggcg ctcaccggga ccaacgcggc
      901 ctggggccag cgcacctgga acgccttcca gctcggctgc gacctcatca acgagcaggg
      961 cggcatcaag tcgatggggg gcgccaagct caagtacatc gtcgcggaca ccgagtccaa
     1021 gccggaggtc gccggctccc aggccgagaa ggtcgtgggg cgcggcgcgg tggccatcac
     1081 cggcaccaac cagagcgcgg ccaccatcgt ggccacccag atcgcggagc gcgagggcgt
     1141 gcccttcgtc tgcgccaccg acgtggaccc gctgatcacc tcgcgcggct tcaagtacac
     1201 cttccgcacc tcgccgctgg tctcggccta cgcggccgac ctgctcgagt acgtcaagga
     1261 gctgggccag aaggcgggca agccggcccg gaccctcgcg atcctgagcg agaactccat
     1321 cgtggggcag tcgtcggtgg agggggccac caaggtgggc aaggagctgg gctacgaggt
     1381 ggtggcgggc gacacctacg atgcgtcgaa gacgcagaac ttcgccagct acatctccaa
     1441 gtacaagggc ggcaacgtcg acgtggtcat cggtcacaac aagccgagcg acgccatcct
     1501 gatcacccgc accatgaagg agctgaacta caacccgatg gcctacgggg gcatcctcgg
     1561 cggccacgtc tccaccgagt acgtgaacgc actcggcaag gacgcggaca acgtcctcgc
     1621 caccacgagc tggtcacccg acgccgacat cgccggcctc aaggagctgg ccaagaagta
     1681 cctcgaccgg ttcaaggagc cgctcgactc caccagcgcg ggcgggttca ccgcgatcgc
     1741 ggtgctgtgg gacgcgctgg agcgggcggg gagcgccgac cgcaagaagc tgcgcgacgc
     1801 cctcgcggcc acccagctca agaccggcga gaagatgtac atgcagctcc ggggggccaa
     1861 gttcacggcc agcggcgaga acgagcgggc cggcggcctc gtgttcgtgg tgaaggacaa
     1921 gacgtgggtc acggtggcgc cgaaggagtt cgccaagacc caggccccct tcccgaagcc
     1981 ccggtggggg gcctagaggc cccggaggcg acgctcagcg ccgacggggc gaccgggacc
     2041 gagccgagat ccgcggcttc ggaaaggcgc cgcgcagtcc cttcgcccgc acctggaaca
     2101 aggtctccgc ctccggcagg ccgggcagac gg
//
LOCUS       DATMCV010000304         5365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_151123, whole genome shotgun sequence.
ACCESSION   DATMCV010000304 DATMCV010000000
VERSION     DATMCV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151123"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VNU03_07065"
     CDS             <1..328
                     /locus_tag="VNU03_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74256.1"
                     /translation="AQDGRALPGSTATLLDDSIKFMHVSAPIRVMERDEWAPSELREF
                     GLDPPGYTAILYRGSTPVLGAEFGAANPQKVLQYMKVQGHDQVYLMSRFIGEEWEKVL
                     RAAVSG"
     gene            325..1389
                     /locus_tag="VNU03_07070"
     CDS             325..1389
                     /locus_tag="VNU03_07070"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HWP74257.1"
                     /translation="MKRPLARTGLGALLVAGAVAGAWAHAGGSTGYASITVSRTTVRY
                     RLTLPTATLPSELAEALRLEQKGSAPSREKLLDLLRTRIVLQADGVRCEPGPGQVLPS
                     SFDATSFTMLVDFACGGLVRELTVQDDIFDVLGLDHHTLAKLEAPNGTQQFAFAPDTR
                     HARFVVGEPPGVARSTGSFFVLGIDHILTGYDHLLFLIGLLLPGGGLLSLAKIITAFT
                     IAHSVTLSLAVLQVVTLPDRLVEAVIALSIAFVAAENLFLKPTVSRRWLVSFAFGLVH
                     GFGFSSALRELGLPAHGLLLSLFGFNVGVEVGQALVVAVCLPCLLLLRRTRWESRAVA
                     SFSLVILVVGLTLFVERALP"
     gene            1442..3214
                     /locus_tag="VNU03_07075"
     CDS             1442..3214
                     /locus_tag="VNU03_07075"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWP74258.1"
                     /translation="MSPAGPRPDIEHHAADRATTRPVVSGTQGVVAAGHPLVAMAGMR
                     MLLGGGNAFDAAVAAGFAAAVVEPTASYTLMGECVALVHEARSRRTLALSGQGTAPAL
                     ATIGAFSGRGLDRIPTGPGSDAHLSFTVPGAVDAYLTLLETLGTKTLPEVLEPAVGYA
                     RRGFPVYEFLHRMLDQPATRAQFAIYPPGGEAVFYPGGRVPPVGALLVQPVLAGTLER
                     LVEAGAGARDRAAGIAAARRRFYSGDIAAAVGAFSERLGGLLRASDLAGYRATLEPPV
                     GITFAGREILAQSAWTQGPVLLQALGMLAGFDLRAMGHNSTRYIHVVTEALKLAFADR
                     ERHYGDSAAVPLAGLLAPGYLRERAGLIRLDRAVPVAPAPGDPGGGGVGAGAPLVARV
                     ADPAPADGTTHIAAIDRDGNMIGLTPSGGVFRKSAFAPELGCTLSTRSEMFVLEEGHP
                     NALAPGRRPRTTLVSYLIVENGEPTVTIGCPGGDDQAQADLQLVLNVLVFGMNPQQAV
                     EAPRFSTQTLVNSFYPRAYHPGELNVEAGIPPATRAELSALGHSVSESGACGIGAIVT
                     RRDPATGVLSAGADPRRPTYAISW"
     gene            3275..3682
                     /locus_tag="VNU03_07080"
     CDS             3275..3682
                     /locus_tag="VNU03_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010491057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWP74259.1"
                     /translation="MASIRREIQVEAGAERVWDAIADVGAIHTRLAPGFVVDTRLEDG
                     ARVVTFGNGLVARELIVDVDHASRRLVWAVVGGRPTHHNASLQVLVEGERRSRVIWIA
                     DLLPDELAGPIASLIDQGMAVMKQTLESRVEVG"
     gene            complement(3696..4574)
                     /locus_tag="VNU03_07085"
     CDS             complement(3696..4574)
                     /locus_tag="VNU03_07085"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP74260.1"
                     /translation="MILVTGANGLNGRALLERLSARGVPARALVRSLAKAEGLSALPK
                     VEIVEGDMARPDTLARALRGVDRAMLISSSDPAMLDVQSSFIDAARAAGVAHVVKLSG
                     IMPELDSPFRFARMHGEIERRLEASGMAFTHLRAGEFMPAYFRQVPSIVAKGALFLPM
                     EDARIASIDVGDIAEVAAVALTTPGHEGKIYPLTGPEALTMTEIAERLSTATGKAIRY
                     VNVAPEDARKAQLAAGMPPYLVDALAELFAERRRGKESHVSPAVETILGRRPTSFAEF
                     ALRNAAVFRGEPPPRA"
     gene            4704..5057
                     /locus_tag="VNU03_07090"
     CDS             4704..5057
                     /locus_tag="VNU03_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP74261.1"
                     /translation="MASSKAAGTDGCPVAAFQKMISGKYKLRIVWDLKDGPLRYGQIR
                     SGLLRGSDATREIAPRVLSRELKALTQTGLIDRRDYGVVPPKVEYRLTAKGRSFVPVI
                     SAIRAWGTRHLRLQS"
     gene            complement(5143..>5365)
                     /locus_tag="VNU03_07095"
     CDS             complement(5143..>5365)
                     /locus_tag="VNU03_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74262.1"
                     /translation="MGLSPILVETIFGIIRDINRLGTTILLVEQNARMALRVAHRGYV
                     IQTGRIVLHDAAPALLHSDVVRKAYLGEK"
BASE COUNT          727 a         1862 c         1960 g          816 t
ORIGIN      
        1 ggcccaggat ggccgcgctc tgcccggctc gacggccact ctgctcgacg actcgatcaa
       61 gttcatgcac gtgtcggccc cgatccgggt gatggagcgg gacgagtggg ccccgagcga
      121 gctgcgggag ttcggactcg atccgcccgg ctacaccgcg atcctctatc ggggaagcac
      181 cccggtgctc ggcgccgagt tcggggccgc gaatccgcag aaggtcctcc agtacatgaa
      241 ggtgcagggt cacgaccagg tctacctgat gtcccggttc atcggcgagg agtgggagaa
      301 ggtgctgcgc gcggcggtaa gcgggtgaag cggccgctcg cccggaccgg gctgggcgcc
      361 ctcctcgtgg ccggagcggt ggcgggcgcg tgggcccacg cggggggaag caccggctac
      421 gcctcgatca cggtcagccg gaccacggtg cgctatcgcc tgaccctgcc gaccgccacg
      481 cttccgtccg agttggccga ggcgctccga ctcgagcaga aggggagcgc gccgagccgc
      541 gagaagctgc tcgacctgct tcgcacgcgg atcgtcctcc aggccgacgg ggtccggtgc
      601 gagccggggc ccggccaggt gctcccgtcg agcttcgatg ccacgagctt cacgatgctg
      661 gtcgacttcg cgtgcggggg cctggtgcgg gagctcaccg tgcaggacga catcttcgac
      721 gtcctcggcc tggatcatca caccctggcg aagctcgagg cgccgaacgg cacccagcag
      781 ttcgcgttcg cccccgacac gcgccacgcg cgcttcgtgg tcggcgagcc gcctggggtc
      841 gcgcgctcga ccggcagctt cttcgtgctc gggatcgacc acatcctgac cgggtacgac
      901 cacctgctgt tcctgatcgg gttgctcctg ccgggcgggg ggctcctctc gctcgccaag
      961 atcatcaccg cgttcacgat cgcccacagc gtcaccctct ccctggcggt gctgcaggtg
     1021 gtcacgttgc cggatcggct ggtcgaggcg gtgatcgcgc tgtccatcgc gttcgtggcg
     1081 gccgagaacc tgttcctgaa gccgaccgtc tcgcgtcgct ggctggtcag cttcgccttc
     1141 ggcctcgtgc acggcttcgg gttctcgtcg gccctccgcg agctcggcct gcccgctcac
     1201 gggctgctgc tctcgctgtt cgggttcaac gtgggcgtcg aggtgggcca ggccctggtg
     1261 gtggcggtct gcctgccgtg tctcctcctt ctccggcgca cacgctggga gagccgggcc
     1321 gtggcgagct tctcgctggt cattctcgtg gtcgggctga ccctgttcgt cgagcgcgcg
     1381 cttccctgaa tcttccatcg aggcctggcc cggctcgctc cgaacgcggt atgctgccgc
     1441 ggtgagcccg gccggtcccc gccccgacat cgagcaccac gccgccgacc gggcaaccac
     1501 gcgcccggtc gtctccggca cccagggtgt ggtggcggcc ggccatcccc tggtggcgat
     1561 ggccggcatg cggatgctcc tcggcggggg caacgccttc gacgccgcgg tggcggccgg
     1621 cttcgccgcc gcggtcgtgg agcccaccgc gtcctacacg ctcatggggg agtgcgtggc
     1681 gctcgtccac gaggcgcgga gccggcgcac cctggcgctg agcgggcagg gcaccgcccc
     1741 ggcgctggcc acgatcggag cgttctccgg ccgcggcctc gatcgcattc cgaccggtcc
     1801 ggggtccgac gcccacctgt cgttcacggt gccgggagcg gtcgacgcct acctcacgct
     1861 gctcgagacg ctgggcacga agacgctgcc cgaggtgctc gagcccgcgg tcggctacgc
     1921 ccggcgcggc ttcccggtgt acgagttcct gcaccggatg ctcgaccagc cagcgacgcg
     1981 cgcgcagttc gcgatctatc cgcccggggg cgaggcggtg ttctacccgg gcggtcgcgt
     2041 ccctccggtg ggcgcgctgc tcgtgcagcc ggtgctcgcc ggcacgctgg agcgactggt
     2101 cgaggccggc gcgggcgccc gcgatcgggc ggcgggcatc gcggccgcgc gccggcgctt
     2161 ctattcgggc gacatcgcgg cggcggtcgg cgcgttctcc gagcggctgg gcggcctgct
     2221 gcgcgcgagc gacctggccg gctaccgggc gactctcgag ccgccggtcg ggatcacgtt
     2281 tgcgggacgg gagatcctcg cccagtcggc gtggacccag ggcccggtgc tgctgcaggc
     2341 cctcggcatg ctggccggct tcgatctgcg cgcgatgggc cacaactcga cgcgctacat
     2401 ccacgtggtg accgaggccc tcaagctggc cttcgccgat cgcgagcgcc actacggcga
     2461 ctccgccgcg gtgcctctgg ccgggctgct ggcgcccggc tacctgcgcg agcgggccgg
     2521 gctcatccgc ctcgatcgtg cggtgccggt ggctccggcg cccggcgacc cggggggcgg
     2581 aggcgtcggg gcgggcgcgc cgctcgtggc gcgggtcgcg gacccggcgc cggccgacgg
     2641 caccacgcac atcgcggcga tcgatcgcga cggcaacatg atcggcctga ccccgagcgg
     2701 tggggtcttc cggaagtcgg cgttcgcgcc ggagctgggc tgcaccctca gcacgcggag
     2761 cgagatgttc gtgctcgagg agggccatcc gaatgccctc gcccccggcc ggcggccccg
     2821 aacgacgctg gtgagctacc tgatcgtgga gaacggcgag cccaccgtca cgatcgggtg
     2881 ccccggcggc gacgaccagg cccaggccga tctgcagctc gtgctgaacg tcctggtctt
     2941 cggcatgaac ccgcagcagg cggtcgaggc gccgcgcttc tcgacccaga ccctggtgaa
     3001 ctcgttctac ccgcgcgcct accatcccgg cgagctcaac gtggaggccg gcatcccgcc
     3061 cgccacgcgc gccgagctct ccgccctcgg gcactcggtg agcgagagcg gggcatgcgg
     3121 catcggggcc atcgtgacgc gccgcgatcc cgccaccgga gtcctctccg ccggcgccga
     3181 tccacgtcgg ccgacgtacg cgatctcctg gtagccgcgt ttcacgaagc gccgccgccg
     3241 gggtctcaga gatgcagggc cggaggaggt cgccatggcg tcgatacggc gggaaattca
     3301 ggtggaagct ggtgcggagc gggtgtggga cgcgattgcc gacgtggggg cgatacacac
     3361 ccggctggct cccggcttcg tggtcgacac gcgtctcgag gacggggcgc gggtcgtcac
     3421 gtttggcaac ggcctggtgg cccgcgagct catcgtggac gtggaccatg ccagccggcg
     3481 gctcgtgtgg gcggtggtcg gcgggcgccc cacccatcac aacgcgtcgt tgcaggtcct
     3541 cgtcgagggc gagcggcgca gccgggtgat ctggatcgcg gacctgctac cggacgagct
     3601 ggcaggaccc atcgcctcgc tcatcgatca gggcatggcg gtgatgaagc agaccctgga
     3661 gagccgcgtc gaggtggggt gagcccgcac cggcgtcagg cgcggggcgg cggctccccg
     3721 cggaagacgg cggcattgcg cagcgcgaac tcggcgaagc tggtgggtcg ccggccgagg
     3781 atcgtctcca ccgccggaga gacgtgcgac tccttgcccc gtcggcgctc cgcgaagagc
     3841 tcggccagcg cgtcgaccag gtacgggggc atgcccgcgg cgagctgggc ttttcgcgcg
     3901 tcctccggag cgacgttgac gtagcggatg gccttgccgg tggcggtgga gagccgctcc
     3961 gcgatctccg tcatggtcag ggcctcgggg ccggtgagcg ggtagatctt cccctcgtgc
     4021 ccgggcgtgg tcagggcgac cgcggcgacc tcggcgatgt cgcccacgtc gatcgaggcg
     4081 atgcgcgcgt cctccatggg caggaacagc gcccccttgg cgacgatcga cgggacctgg
     4141 cggaagtagg cgggcatgaa ctcgcccgca cgcagatggg tgaacgccat cccggacgcc
     4201 tccagcctcc gctcgatctc gccgtgcatc cgggcgaagc ggaacgggga atcgagctcc
     4261 ggcatgatcc cggagagctt gacgacgtgt gccacccccg cggcgcgagc ggcgtcgatg
     4321 aagctcgact ggacgtcgag catggcggga tccgaggagg agatcaacat cgcgcgatcg
     4381 acgccacgca aggcccgggc gagcgtgtcg ggccgcgcca tgtcgccctc cacgatctcg
     4441 accttgggga gcgcggacag cccctcggcc ttggcgaggc tgcgcaccag cgcgcgggcg
     4501 ggaacgcctc gtgccgagag ccgctccagc agcgctcgtc cattcaatcc gttggcaccg
     4561 gtcacgagga tcatcgtccg cctccctgac tccgtctcgg tcgttgcgag tgctatgatt
     4621 tggccccggt aggagcgatt aggcaagtac ggacaaaaac gatactgtcg gagaccgatg
     4681 ggacgggagg aggacgcccg acgatggcca gctcgaaggc ggcggggacc gatgggtgcc
     4741 cggtcgccgc gttccagaag atgatcagtg gcaagtacaa gctccggatc gtctgggacc
     4801 tgaaggacgg tccgctgcgg tacggtcaga tccggagcgg tctcctgaga ggctcggacg
     4861 cgacccggga gatcgccccg cgcgtcctga gtcgcgaatt gaaggcgctc acccagaccg
     4921 ggctcatcga ccggcgagac tacggggtcg tgccccccaa ggtcgagtac cgcttgaccg
     4981 ccaaaggtcg aagcttcgtg ccggtcatct cggcgattcg cgcctggggc acgcgccacc
     5041 tgaggctaca gagctgatgt gtcgaagccg tcccaggtac gaagcagtgg caggacacct
     5101 cgtacgctgg gggccggtag cccgcggggc gtgctcggca cgctatttct cgccgaggta
     5161 ggccttgcgc accacgtcgg agtgcagcag agccggggcg gcgtcatgca gcacgatgcg
     5221 tccggtctgg atgacgtagc cccgatgcgc cacccgcagc gccatgcgcg cgttctgctc
     5281 cacgagcagg atggtggtcc cgagccgatt gatgtcgcgg atgatgccga agatggtctc
     5341 gaccaggatc ggagagaggc ccatc
//
LOCUS       DATMCV010000305         2233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_15012, whole genome shotgun sequence.
ACCESSION   DATMCV010000305 DATMCV010000000
VERSION     DATMCV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15012"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2233)
                     /locus_tag="VNU03_07100"
     CDS             complement(<1..>2233)
                     /locus_tag="VNU03_07100"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP74263.1"
                     /translation="LSRLLGILAEEGVLQRDGTGWRVVAPLPEADVEAAERALLALFP
                     AGAPEATVTMDCGRALAAVLRGQTDPLQILFGPETRANIERLYSDTPAARTFNALVAD
                     AVAGAIGTAPRARRVRILEIGAGTGGTTGAILSALPPEATEYVFTDVSPAFTTRAADR
                     LGAPNRSFRPLDIERDPAAQGFAGERFDIVVAANVIHATRDLRRTLGHVRDLLAPGGL
                     LLLLEGTARYRWVDLTFGLTDGWWHFTDLDLRPEHALLDGERWIELLRACGLQDPARL
                     PARPSGRALAGQTVLIARAPAAEPAGITSGRDWLVLADQGGIGRRLVSSLEARGQRVR
                     ALEPGDAARIAEAVRSGAGWHAVVDLRALDAGSATSAEVASRLHAQALDLVKVLAGAS
                     GVPRLWLVTRGAQPAGDGQESVAVAQAPLWGLGRVVALEQPELWGGLVDLDSSDTPAE
                     AAERLAEQLADPDEEDQLAFRAGHRMVPRLVRGDAPGDQPLAVHGDGTYLITGGLGGL
                     GLKIARWLVEQGARHLVLTGRRGLPAREAWAAMPADDRRSAQIEAIRELEQLGARVDT
                     VAADVAERAAMAALFASFGSTRPALRGIVHAAADVRGEPLAEATAEVRDAVLAPKVTG
                     SWLLHELSRALPLDFFVLFSSTTALLGSALLGHYAAANQFLDALAHHRRREGLPALSV
                     NWGTWDEMRVATAADRRAFAQGGLLPMRSDDALALLGRLAAGPAAQVTVAAVDWDALI
                     ALYE"
BASE COUNT          308 a          855 c          814 g          256 t
ORIGIN      
        1 cctcgtagag cgcgatgagg gcgtcccagt ccaccgccgc caccgtgacc tgcgccgcgg
       61 ggccggcggc caggcggccg agcagcgcga gcgcatcatc ggagcgcatc ggcagcaggc
      121 cgccctgggc gaacgcgcgc cggtcggcgg ccgtcgccac ccgcatctcg tcccaggtgc
      181 cccagttcac gctgagggcc ggcaggcctt cgcggcggcg gtggtgggcg agcgcatcga
      241 ggaactggtt ggcggccgcg tagtggccca gcagggccga ccccagcagc gcggtggtcg
      301 acgagaacag cacgaagaag tcgagcggca gggcgcggct cagctcgtgc agcagccacg
      361 agccggtcac cttcggcgcg agcaccgcgt cgcgcacctc cgcggtcgcc tcggcgagcg
      421 gctcaccgcg gacgtccgcc gcggcatgca cgatgccgcg gagcgccgga cgcgtcgacc
      481 cgaacgaggc gaagagcgcg gccatcgcgg cgcgctcggc aacatcggcc gccaccgtat
      541 cgaccctggc tccgagctgc tcgagctccc tgatcgcttc gatctgggcc gagcggcggt
      601 cgtccgcggg catcgcggcc catgcctccc gggcgggcaa gcctcggcgc ccggtcagga
      661 ccaggtgccg cgcgccctgc tccaccagcc accgcgcgat cttgagaccc aagccgccga
      721 ggccgccggt gatcaggtac gtgccgtcgc cgtggaccgc gagcggctga tccccgggcg
      781 cgtcaccgcg gacgaggcgc ggcaccatgc ggtgaccggc tcggaaggcg agctgatcct
      841 cttcgtcggg atcggccagt tgctccgcga ggcgctccgc cgcttcggca ggggtgtccg
      901 aggaatcgag atccacgagc ccgccccaga gctcgggttg ctccagcgcg acgacacgac
      961 cgagccccca gagcggcgcc tgggcgaccg ccacggactc ctgaccatcg ccggccggct
     1021 gcgccccgcg ggtcaccagc cagaggcgcg gcacgccact cgcgcccgcc agcaccttga
     1081 ccaaatcgag agcctgcgcg tggagcctgc tcgcgacctc cgcgctcgtg gcggagccgg
     1141 catcgagggc ccggaggtcg accaccgcgt gccagccggc tccgctccgg accgcctccg
     1201 cgatccgggc ggcgtccccg ggctcgagcg ctctcacccg ctggccgcgg gcctcgagcg
     1261 acgatacgag ccgccgaccg attccaccct ggtccgccag caccagccag tcgcggccgc
     1321 tcgtgatgcc cgccggctcc gccgccggag cccgcgcgat cagcaccgtc tggcccgcga
     1381 gcgctcgccc cgacggccgc gccggcagcc gcgccggatc ttgaagcccg caagcgcgga
     1441 gcagctcgat ccagcgctcg ccgtcgagca gcgcgtgctc ggggcgcagg tcgagatccg
     1501 tgaagtgcca ccatccgtcg gtgagcccga acgtgaggtc gacccagcgg tagcgcgcgg
     1561 tgccttccag cagcagcagg aggccaccgg gcgcgagaag atctcgcacg tggcccaggg
     1621 tccgccgcag gtcgcgggtg gcgtggatca cgttggccgc caccacgatg tcgaaccgct
     1681 cacccgcgaa gccctgtgcc gccggatcgc gctcgatgtc gaggggccgg aagctccggt
     1741 tcggcgcccc gagacgatcg gccgcgcgag tggtgaaggc aggagagacg tcggtgaaca
     1801 cgtactccgt cgcctccggc ggcagcgcgg acaggatggc gcccgtggtg ccgccggttc
     1861 cggcgccaat ctccaggatg cggacccggc gggcccgcgg cgcggtcccg atcgcgccgg
     1921 ccaccgcgtc cgcgacgagg gcgttgaacg tgcgtgcggc cggcgtgtcg ctgtagagcc
     1981 gctcgatgtt cgcgcgcgtc tcgggcccga acaggatctg cagcggatcg gtctggccgc
     2041 gaagcacggc ggccagggcg cggccgcagt ccatcgtcac ggtggcctcc ggcgctcccg
     2101 ccgggaacaa cgcgagcagc gcgcgctcgg cggcctcgac gtcggcctcc ggcagtggcg
     2161 cgaccacgcg ccagcccgtt ccgtcgcgct gcagcacgcc ttcctcggcc aggatgccga
     2221 ggagccggga cag
//
LOCUS       DATMCV010000306         3269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_349401, whole genome shotgun sequence.
ACCESSION   DATMCV010000306 DATMCV010000000
VERSION     DATMCV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349401"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VNU03_07105"
     CDS             complement(<1..229)
                     /locus_tag="VNU03_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWP74264.1"
                     /translation="MTRLAESSEQRYRALVESSRGLICTHDMAGILLSVNRAAADRLG
                     YAADELVGRSLGDVLAPSVRAQFPQYLERLAH"
     gene            complement(242..616)
                     /locus_tag="VNU03_07110"
     CDS             complement(242..616)
                     /locus_tag="VNU03_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP74265.1"
                     /translation="MPDPRILVVEDETNVRELEAEILREEWEHVDTAVDGTDALRLLA
                     EHVYAAIVSDLAMPKMDGRGLYEEVGRRYPSAHGRLVFVTGYAEAEEYDAFLRQSGVP
                     VLAKPFTIADLIGAVQRAFSPA"
     gene            complement(812..2191)
                     /locus_tag="VNU03_07115"
     CDS             complement(812..2191)
                     /locus_tag="VNU03_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP74266.1"
                     /translation="MPPQSLKIDHARYVLTLDRQRRIIQDGAVLVENGRISRVGKAAE
                     LAEARADRVIDGRHLVITPGFFNGHMHISYAHPVRGIFPDEQASPLVHVFNLQAAMTE
                     EEEYHTTLLGLVELVRNGTVCFLDPGSTKFPDACLQAYEDAGIRVVLGECVTDQPAPW
                     ALPRYPAPEAVARTSSFLAKWNGRLDGRIRAWAMPFSYETCSAGLLESLKRVVDEQHT
                     GLTLHHNSGTKARQDAVARHGHSPTAYLESIGVLGPNVLLAHCLGLDEAELDCIARTG
                     TTVAMCPVTAAKGGRGVPEHGRMPELLARGVRIALGCDSPNNSNHLDMVRTMNMAAIQ
                     YKDARQDTRQIPAEAALEMATIHGAEALGLGEELGSIEVGKKADLVAFDILRPEWQAL
                     WNPVNNLVYNADGRSVHTVVIDGRVVVDAYRQTFVDEPRLYARVQDIGERLQARTGIS
                     FPRSRWPLS"
     gene            2302..3189
                     /locus_tag="VNU03_07120"
     CDS             2302..3189
                     /locus_tag="VNU03_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWP74267.1"
                     /translation="MPLAHVALALLVTIIWGVAFVATRLALDDFSPAALTALRFLVAA
                     LPAALLPRPRVSWRALVLIGLTLYTGQFLFQFLGIAAGMPPGLAAIVVQTQALFTIVL
                     AALAAGERPTGREWSGTAVAFAGLAVIAATVGGDLTAIGLGLTAMSAVSWAVGNVLVK
                     RLPAVDPLALAVWLSLVPPLPGLALAALWDGPRALGASARGASWLTVAAVLYLGLVAT
                     VLAYAIWGFLLRRHPAARVAPFALLVPFVATASSALVLGERFGPHRLVGMGLVLLGLA
                     IIMVRPGAAGVTEPARRSR"
BASE COUNT          390 a         1184 c         1165 g          530 t
ORIGIN      
        1 cgtgggcgag gcgctcgagg tactggggga actgggcccg caccgacggc gccaggacgt
       61 cgcccaggct ccggccgacc agctcgtcgg ccgcgtagcc caggcgatcg gcggcggccc
      121 ggttgaccga gagcaggatg cccgccatgt cgtgggtgca gatcaggccg cggctcgact
      181 ccaccagcgc tcgatagcgc tgctcggacg attcggcgag acgggtcacc ggctggctcc
      241 gctaggcggg cgagaacgcc cgctggaccg ccccgatcag gtcggcgatc gtgaacggct
      301 tggccagcac cggcacgccg ctctggcgga ggaaggcgtc gtactcctcg gcctccgcgt
      361 agccggtcac gaacacgagg cgcccgtgcg cgctcgggta gcggcgcccg acctcctcgt
      421 agagcccgcg gccgtccatc ttgggcatcg cgagatcgct cacgatcgcg gcgtagacgt
      481 gctcggcgag cagccggagg gcgtccgtgc cgtcgactgc ggtgtcgacg tgctcccact
      541 cctccctcag gatctccgcc tccagctcac ggacgttcgt ctcgtcctcg actaccagga
      601 tgcgcgggtc gggcactggc ttctcctctc ggggttggac gtccgggctc gagcgttggg
      661 gccggcaagg gcgtcggccc ggctcaagta gtgcaaggac tgcaccatga agcggctcag
      721 cgaaaacagg gagttagtcg gccatccctg cgtcgggact gatgggaaat gtcagccctc
      781 gcgcaattgg gcagccggcc cgctcgtcgc ctcagctgag gggccagcgg gagcgcggga
      841 agctgatgcc ggtccgcgcc tgcagccgct ccccgatgtc ctgcacccgg gcgtagagcc
      901 gaggctcgtc gacgaaggtc tggcggtagg cgtcgaccac cacccgaccg tcgatcacca
      961 ccgtgtgcac gctgcggccg tcggcgttgt agacgaggtt gttgaccggg ttccagaggg
     1021 cctgccactc cgggcgcagg atgtcgaagg ccaccaggtc cgccttcttg ccgacctcga
     1081 tggagcccag ctcctccccc aggccgaggg cctccgcgcc gtggatcgtg gccatctcga
     1141 gcgcggcctc ggccgggatc tgccgggtgt cctggcgcgc gtccttgtac tggatggcgg
     1201 ccatgttcat ggtgcgcacc atgtcgaggt ggttcgagtt gttcggcgag tcgcagccga
     1261 gggcgatccg gaccccgcga gccagcagct cgggcatacg cccgtgctcg ggcacgccgc
     1321 gtccgccctt ggccgcggtg accgggcaca tggccaccgt ggtgccggtg cgggcgatgc
     1381 agtcgagctc ggcctcgtcg agccccaggc agtgggcgag tagcacgttg ggccccagca
     1441 ccccgatcga ctcgaggtag gcggtgggac tatggccgtg ccgcgcgacc gcgtcctggc
     1501 gcgccttggt cccgctgttg tggtgcaggg tcagcccggt gtgctgctcg tcgacgacgc
     1561 gcttcaggga ctcgagcagc cccgcgctgc aggtctcgta ggagaacggc atcgcccagg
     1621 cgcgaatccg gccgtcgagg cgcccgttcc acttcgcgag gaacgagctc gtccgcgcca
     1681 ccgcctccgg cgccgggtag cgcggcaggg cccacggcgc gggctgatcg gtcacgcact
     1741 cgcccagcac cacccggatg cccgcgtcct cgtaggcctg cagacacgcg tcggggaact
     1801 tggtgctccc gggatcgaga aagcacaccg tcccgttccg gaccagctcg accaggccga
     1861 gcagcgtggt gtggtactcc tcctcctcgg tcatcgcggc ctggaggttg aagacgtgca
     1921 cgagcgggct cgcctgctca tcggggaaga tgccgcgcac cgggtgcgcg tagctgatgt
     1981 gcatgtggcc gttgaagaag cccggggtga tcacgaggtg ccggccgtcg atcacccggt
     2041 cggcgcgcgc ctccgcgagc tcggccgcct tccccacccg gctgatgcgt ccgttctcca
     2101 ccagcaccgc accgtcctgg atgatgcggc gctgccggtc gagggtcagg acgtagcggg
     2161 cgtggtcgat cttgaggctc tgcggtggca tgggggcggc ctccctggct gagtgcctga
     2221 gcgagtggtg aacgcccgga gtctacgccg ccgccccgcg gcttgtccat cgcgcggccg
     2281 ccggatgcta tggtcgagcg cgtgccgctc gcccacgtcg cgctggccct gctggtgacg
     2341 atcatctggg gcgtggcctt cgtggccacg cgcctggccc tcgacgattt ctcgcccgcc
     2401 gcgctcaccg cgctgcgctt cctggtggcc gccctgcccg ccgcactgct gccgcgccct
     2461 cgcgtctcgt ggcgggcgct ggtgctgatc ggcctcaccc tctacaccgg gcagttcctg
     2521 ttccagttcc tcggcatcgc ggccggaatg ccgcccgggc tcgccgcgat cgtggtgcag
     2581 acccaggccc tcttcaccat cgtgctcgcg gccctcgcgg cgggcgagcg gcccaccggc
     2641 cgtgagtgga gcgggaccgc ggtggccttc gcgggcctgg ccgtcatcgc ggccacggtg
     2701 ggaggcgacc tgaccgcgat cggtctcggc ctcaccgcga tgtccgcggt gagctgggcc
     2761 gtcggcaacg tgctggtcaa gcgcctcccc gcggtcgacc cgctcgccct cgcggtctgg
     2821 ctgagcctcg tgccgcccct gcccggcctg gcgctcgccg cgctgtggga cggcccgcgc
     2881 gccctcgggg cctcggcgcg cggcgcctcg tggctcacgg tggccgcggt cctctacctg
     2941 ggcctcgtcg ccaccgtgct cgcctacgcg atctggggct ttctcctgcg ccggcatccc
     3001 gccgcgcggg tggcgccctt cgcgctcctc gttcccttcg tggcgaccgc gtcgtccgcg
     3061 ctcgtgctcg gcgagcggtt cgggccgcac cggctcgtcg gcatgggatt ggtgctgctg
     3121 ggcctcgcga tcatcatggt gcgaccggga gcggcaggag ttacggagcc ggctcgtcgt
     3181 tcccggtaag ccttacccgc gcgcctcccc gacgcccgcc cgatgcgcgt caaacacgcg
     3241 atctgcgcgt cttgcacggg aaatgcttc
//
LOCUS       DATMCV010000307         2256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_751343, whole genome shotgun sequence.
ACCESSION   DATMCV010000307 DATMCV010000000
VERSION     DATMCV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751343"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VNU03_07125"
     CDS             complement(<1..1011)
                     /locus_tag="VNU03_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP74268.1"
                     /translation="MLDLVIRNARIVDGTGAPAFMGDLGVAGGKIRYVGPSSGQTAER
                     VLDADGLVLAPGFVDPHTHYDAQIAWDPMLTCSPWHGVTTVIMGNCGVGVAPVRPETR
                     EILMQDLVNVEAIPYDVMKAGIDWQWESYGQYLDAVGRGGLGINVAGLVAFTPLRHYV
                     MGEASFERAATPDEIASMRRLLREAMEAGAFGFTTTTSRNHVGYQGRPLACRNASREE
                     LIGVVAGLHDVGRGAIEIILNSGGMYAVSDDDVDLLRQITRASGRPVTWLALFAHPGE
                     PGFHDQTFAKLGDLVKQAIPQVTPRPIMSQGDLKSPTMFGSFVSWQKAFNRTPAEQMA
                     LYR"
     gene            1151..>2256
                     /gene="chrA"
                     /locus_tag="VNU03_07130"
     CDS             1151..>2256
                     /gene="chrA"
                     /locus_tag="VNU03_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013489379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HWP74269.1"
                     /translation="MPSGRLSAALEVLAVATRLGLTSFGGPVAHLGYFHREYVVRRRW
                     LDEPTYADVVALCQFLPGPASSQVGIAVGILRAGLPGGLAAWLGFTLPSALALVLFAL
                     FIRGADLSGAGWLLGLKIAAVAVVAQAVWGMARALCPDRERASIAIAAALVVTAWPGA
                     WGQVAVIALAGVIGLGLLPAAADDSPTRVRVPIGRSLAAAALTLFFALLLVLPILRYT
                     TGAHALAVFDSFYRAGALVFGGGHVVLPLLQAEVVPPGWVTSEEFLAGYGAAQAVPGP
                     LFTLSAYLGAVMRGRPSGWLGAALALTAIFLPAFLLVVGALPFWDALRGRPRFRAALR
                     GINAAVVGLLLAALYHPVWTSAIHGPADFALALAA"
BASE COUNT          244 a          840 c          811 g          361 t
ORIGIN      
        1 gcggtagagc gccatctgct cggcgggcgt gcgattgaac gccttctgcc acgagacgaa
       61 ggagccgaac atggtcgggc tcttgagatc gccctggctc atgatcggcc gcggcgtcac
      121 ctgcgggatg gcctgcttca ccaggtcgcc gagcttggcg aaggtctggt cgtggaagcc
      181 cggctcaccc gggtgggcga agagggccag ccacgtcacc ggccgcccgc tcgcgcgcgt
      241 gatctgccgc agcagatcga cgtcgtcgtc gctcaccgcg tacatgccgc ccgagttcag
      301 gatgatctcg atcgcgccgc ggccgacgtc gtggaggccg gccaccaccc cgatcagctc
      361 ctcgcggctc gcgttgcggc aggccagcgg ccggccttgg tacccgacgt ggttgcgcga
      421 ggtggtggtg gtgaagccga aggcgcccgc ctccatggcc tcccggagca gccgtcgcat
      481 gctcgcgatc tcgtccggag tggccgcccg ctcgaacgag gcctcgccca tcacgtagtg
      541 gcggagcggc gtgaaggcga ccaggcccgc cacgttgatg ccgagcccgc cccggcccac
      601 cgcgtcgagg tactggccgt agctctccca ctgccagtcg atgcccgcct tcatgacgtc
      661 gtaggggatg gcctcgacgt tgaccaggtc ctgcatcagg atctcgcgcg tctcgggacg
      721 caccggcgcc acgccgacgc cgcagttgcc catgatgacg gtggtgacgc cgtgccaggg
      781 cgagcaggtg agcatcgggt cccacgcgat ctgcgcgtcg tagtgggtgt ggggatcgac
      841 gaagccggga gcgagcacga gcccgtcggc gtccagcacc cgctccgcgg tctgcccgct
      901 cgacgggccc acgtaccgga tcttgccgcc cgccaccccc aggtcgccca tgaaggcggg
      961 tgccccggtg ccgtcgacga tgcgcgcgtt cctgatcacg agatcgagca tggcgctccc
     1021 cttttctcca cgtcgggcga tgcaggccgg cggcccgggt gggtcgccga cggctcccgt
     1081 gcgtcaacaa ctatagagga tggcgagtcc cggcgacggg gtcaagccta tatactcgcg
     1141 gcaccgagcg atgccgtccg gccgcctctc cgccgctctc gaggtcctcg cggtcgccac
     1201 ccgcctgggc ctcacctcct tcggcggccc ggtggcccat ctcggctact tccaccgcga
     1261 gtacgtcgtg cggcggcggt ggctcgacga gccgacgtat gccgacgtgg tggccctctg
     1321 ccagttcctg cccggccccg cgagcagcca ggtcggcatc gcggtcggga tcctccgcgc
     1381 cggcctgccg ggcgggctgg ccgcctggct cggcttcacc ctgccgtcgg cgctggcgct
     1441 ggtgctgttc gccctgttca tccggggcgc cgatctctcc ggagccggct ggctgctcgg
     1501 cctcaagatc gcggcggtgg cggtggtggc gcaggcggtg tgggggatgg cccgcgcgct
     1561 ctgccccgac cgcgagcgcg cctcgatcgc gatcgcggcc gcgctcgtgg tgaccgcgtg
     1621 gccgggggcg tggggccagg tggcggtcat cgcgctggcc ggcgtgatcg ggctcgggct
     1681 cctccccgcc gccgccgacg acagcccgac gcgcgtgcgc gtgccgatcg gccggtcgct
     1741 cgcggccgcg gccttgacgc tgttcttcgc gctgctgctg gtcctgccga tcctgcgcta
     1801 tacgaccggc gcgcacgccc tcgccgtctt cgacagcttc tatcgcgccg gcgcgctcgt
     1861 cttcggaggc gggcacgtcg tgctgcccct cctgcaggcc gaggtcgtgc cgcccggctg
     1921 ggtgacgagc gaggagttcc tcgccgggta cggcgcggcc caggcggtgc cgggcccgct
     1981 cttcaccctc tccgcctacc tgggcgcggt catgcgcggg cggcctagcg gctggctcgg
     2041 cgcggccctc gcgctcaccg caatcttcct ccccgcgttc ctgctcgtgg tgggcgcgct
     2101 gccgttctgg gacgcgctgc gggggcggcc gcggttccgg gcggcgctgc gcgggatcaa
     2161 cgcggcggtc gtcggcctcc tgctcgccgc gctctaccac ccggtgtgga cgagcgcgat
     2221 ccacgggccc gccgacttcg cgctggccct cgccgc
//
LOCUS       DATMCV010000308        19408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_418716, whole genome shotgun sequence.
ACCESSION   DATMCV010000308 DATMCV010000000
VERSION     DATMCV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 19408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19408
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418716"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..2363)
                     /locus_tag="VNU03_07135"
     CDS             complement(255..2363)
                     /locus_tag="VNU03_07135"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HWP74270.1"
                     /translation="MRSLTASWRVLLAGSLAGLAVVVLATVNPLRFFEIAELKALDAQ
                     FSIRGPKTPASPIVVVTIDEDDFDGLNLAWPWPRAVHAKFLDLVGRGNPAAIGMDILF
                     TEPSLHGPSDDVALGAAVDRIRDRIVLAAALTTVGDASFVKQALNPPIPEIRGKAAFG
                     SADYDSDADAFVRRATLRRDFQDRDWLGFDMLIYRLAEKAGLTRAPAPSGHELLVNYR
                     GGPGTFPMVAYHRIVTGEVPPEVFAGKIVLVGATTPTLHDIFPTPFASQSGMPGVEIH
                     AHTLETLLQGIPLRRASRLLVPVVAVLAAAGAAWTATALRPLPAFGIVAAAIGGYLAA
                     SHAAFLWARYWVEVVPVPLALLATYAGTVAKNFTQEQREKRRLSRFFSPDVVAEIIRH
                     KDDSTLAATRRRVTVLFSDIRGFTSMSEKMQPEEVVTFLREYLTVMTEAVFKHGGTVD
                     KYIGDAIMALYNVPLEAPDHALRAVRTALEFQERVRPLGERFAARHGGHLACGVGIHT
                     GDAVVGTIGSEQRLEYTAIGDTINLGSRLEGITKEFSVPIIISEGTYAEVREHFAVRD
                     LGEVTVKGKAIPVKIYAVLGELGPAPDDAHREPAGFNSRTAPRRRQEGRVSISDGELT
                     VVAEASDLSRTGIALRAVPRRLEPGEIVKLQMELGPGAPTVTVDRAEVMWSRKDTAGL
                     RFLDLSPTARTTLDTLTSKP"
     gene            2426..2836
                     /locus_tag="VNU03_07140"
     CDS             2426..2836
                     /locus_tag="VNU03_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74271.1"
                     /translation="MRVRSILLAAFWVAMFIWVGYNGMQAISSYFQTNDIAEQAFRDA
                     SDKQRQRNPGELVSSDFLADLRTGVLAGARRQGVQLDPSTLKVSAEGALVRVNLGWTY
                     RTWPLTMGGFDTALPVPMWLGRSFDPQLGMRRLF"
     gene            2850..4238
                     /locus_tag="VNU03_07145"
     CDS             2850..4238
                     /locus_tag="VNU03_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP74272.1"
                     /translation="MSERIIIAEDDEDLAFVLREALRRQRYDVEVAPTAGGLLDGLKA
                     APYDLILLDVRLPDMDGLDAIPRCRELAPDTPIIVMTAHGTRQTAMDAITRGAYDFFT
                     KPLKMDEFQVVVARALDRRRLQKQVKALGDSQPSGLDGLIGQSEALERVVDMAHRAAP
                     TDLTILVQGESGTGKEVLARAIHRLSARKDGPFVPVNCAAIPEGLLESELFGHERGAF
                     TGAVRARPGRFELAKEGTLFLDEIGDMPLSMQAKILRVLQEREFERVGGTRTMTADVR
                     VIAATHRDLDAAVAQGTFRQDLFYRLQGVGIVLPPLRERVDDLPLLATHLLERAAQRL
                     TRQPAVLSPEALRCLWTYSWPGNVRELQHVLEGAMVLSDGVIRPDHLPPAIQRAVQAR
                     PAAEAGPVLSGSLDEALEEWERRMILDALQQTQGVQARAAKILGVSERSLWYRIKKLG
                     IQVRTPDDAPSA"
     gene            4281..5144
                     /gene="galU"
                     /locus_tag="VNU03_07150"
     CDS             4281..5144
                     /gene="galU"
                     /locus_tag="VNU03_07150"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HWP74273.1"
                     /translation="MKVRKAVFPAAGLGTRFLPATKAQPKEMLPLVDKPTIQYVVEEA
                     VASGLEEIILVTGRNKRAIEDHFDAAFELEYYLQDRGKMEELAQIKTISELASVSYVR
                     QKEPLGLGHAILCAKPLVGDEPFGVFLGDDIIVSRVPCMRQLIDVFERHGGPVLAVMR
                     MPREEIGRYGVIAARGLGANVYEVQDLVEKPDPRDAPSDLAIIGRYVLTPDLFGILKE
                     TAPDSRGEIQLTNGLRVLRGRRPIYAVEFEGKRHDTGEKLGFLKATVEFALARPDLSD
                     AFREYLRSLKL"
     gene            5276..6493
                     /locus_tag="VNU03_07155"
     CDS             5276..6493
                     /locus_tag="VNU03_07155"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWP74274.1"
                     /translation="MKSQPKRTFHTASEPEIKAGEVSDVYFDRTVEILRAKGLNKHVK
                     AEIRLKSFPQPDWTFGVLAGIEEASGLLEGLPVNVWAMDEGTLFGPEDTVLLIEGIYP
                     EWAKYETALLGFLCQASGIATKAARCKRAAGPRQVISFGARRMHPALAPMIERNAFVG
                     GCDGVAVTKSAELIDADPTGTIPHALVLLFGDTVEALRAFHEVVDAKVRRVALIDTLQ
                     DEKFEAIRVAEALGRELYAVRLDTPSSRRGDFFKILEEVRWELDLRGFDYVKILASGG
                     IDEYEILKLNPLVDGYGVGTSIANAPVLSFALDIMEIEGVPMAKRGKRSGAKQVWRFP
                     GTVQNTVLRAGAPAPVGPDGRAAEALLKPLIAGGRLVRDLPPPRTLREYVMEQMARID
                     ITAGRNPGLRGDY"
     gene            6498..7283
                     /gene="trpC"
                     /locus_tag="VNU03_07160"
     CDS             6498..7283
                     /gene="trpC"
                     /locus_tag="VNU03_07160"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWP74275.1"
                     /translation="MGVLDEIVAHKRAEVAELKARRPMATVVGACRGLASARDFEAAL
                     RPGPGARVRLIAEVKRASPSQGLFRADLDPIAQAATYAAAGAAAVSVLTDARYFHGSL
                     DDLVQVRGAVPVPVLRKEFIVDEYQLWEAREAGADAVLLIVAALEDAALRDLLSAAKG
                     VGLATLVEVHTAGELDRALRLGAPVIGVNNRDLQTLTTTLAPSLALLPQIPLGPIAVS
                     ESGLRTGADVAQVVAAGAHAVLVGETLLRADDVAAKVRELSLR"
     gene            7280..7900
                     /locus_tag="VNU03_07165"
     CDS             7280..7900
                     /locus_tag="VNU03_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWP74276.1"
                     /translation="MRIKICGITNVDDARLCVEAGADALGFIFVPNTPRCVTTETAAL
                     IIAALPPFVTPVGVFWDHAPGQVKAVAEECRLGALQFHGDEAPEDLLEHRLPVIKTLK
                     VAGAADLARMADYRVAAFLLDSPSRWSEGESRPPIPWEVAAAAARRHPVLLSAGLTPD
                     NVSLAVRRVRPYGVDVNSGVEARPGRKDPVRVRRFVTEARAAAEQP"
     gene            7897..9150
                     /gene="trpB"
                     /locus_tag="VNU03_07170"
     CDS             7897..9150
                     /gene="trpB"
                     /locus_tag="VNU03_07170"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWP74277.1"
                     /translation="MSATRVRPAALPDAGGHFGRFGGRFVPETLMQPLVDLEKAYRAA
                     RGDARFRRRLRELLSTYAGRPTPLYFAERLTRHVGGARIYLKREDLCHTGAHKINNVL
                     GQALLAQRMGKPRVVAETGAGQHGVASATAAALFGLECQVYMGRVDVERQALNVFRMK
                     LLGATVIPVESGSRTLKDAVNEALRDWVTNVRTTYYLLGSVMGPHPYPMMVRDFHRVI
                     GEETRAQARRATGRLPDLLVACVGGGSNAIGLFWPFIGDRRVRIVGVEPGGHGIKSGK
                     HGASLGSGAVGVLHGSMSYVLQNDDGQVAEAHSISAGLDYPGVGPEHSYYKDTGRFEY
                     VSVTDAEALEGFQVLTRLEGIMPALESAHAVAHAMRAAARMKKTQTVVVGLSGRGDKD
                     VHTVGEALDARPGNGLGGNGHGGAR"
     gene            9147..9974
                     /gene="trpA"
                     /locus_tag="VNU03_07175"
     CDS             9147..9974
                     /gene="trpA"
                     /locus_tag="VNU03_07175"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWP74278.1"
                     /translation="MKAATGRAPSRLDATFAALRDRHERALVAYFTAGDPSLALTRKL
                     VVEAARRGADVIELGIPFSDPLADGPVIQRATQRALAAGVTLPRVLELVREMRGEVSV
                     PLVFLTYYNPILAFGLKAFCQTSVEAGIDGVIVADLPPEESGPLRSEAMAAGLDLVHL
                     VAPTSTPERMRKIARASEGFIYLVALTGVTGERTALAPELAQQVRALRAITTKPVCVG
                     FGIGTPEQAALVGQLADGVVVGSAIVRLVEQHVGSPELLARVGDFIAGLKAPLRGGS"
     gene            9978..10844
                     /gene="accD"
                     /locus_tag="VNU03_07180"
     CDS             9978..10844
                     /gene="accD"
                     /locus_tag="VNU03_07180"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HWP74279.1"
                     /translation="MAWFWKKKEDGDEDRPKKVVIAEGLWVKCDSCREIVYRAEVERA
                     GRVCPKCGYPFRISARERIASIVDEGSFEEREGGLRSKDPLSFKDTKRYSDRLKAARA
                     KSSMEEAVITGTARIGGYPVALAVFEFAFLGGSMASVVGEKLTRTIELALQKRVPLLI
                     VSASGGARMQEGILSLMQMAKTSAALARLAAERVPYISLLTDPTTGGVTASFAMLGDL
                     ILAEPRALIGFAGPRVIAETIRQPLPDGFQRSEFLLEHGQLDLVVERRELRATLRRIL
                     AFFGGRDPAPAP"
     gene            10859..12133
                     /locus_tag="VNU03_07185"
     CDS             10859..12133
                     /locus_tag="VNU03_07185"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HWP74280.1"
                     /translation="MARILGLRGGERVGMRPGLERIEALLDAIGRPERAFTIAQVGGT
                     NGKGSISAMLAAILQASGRRVGLYTSPHLRHYRERIRVDGQPISEADFVDGVDALGTL
                     IARLDVTVFEAGTALALDHFCRSRVDVAVLEVGLGGRLDATTVGRPAVVVLGPIDYDH
                     QLELGDTLTAIAGEKAAIIRSGVAFSARQDPEAARVLEERAARVGVPLVREGHELHVT
                     PRGFTLEAQRLDLAGPDWHLADVACGLLGVFQPGNALLAAAAGRALGADEPAIRAGLR
                     GARWPGRFQVFRRDPVVILDGAHNPAGARALAASLRAYFPGRPVTFVIGILADKDAAG
                     ILGALSPLAAGVVLTTSGNPRSAAPASLRALLPADLRVEVAPSPSEALARATAGAAGG
                     LVCVAGSLSLIGDVLAAAGDGQDVLFTAGARC"
     gene            12175..14379
                     /gene="lptD"
                     /locus_tag="VNU03_07190"
     CDS             12175..14379
                     /gene="lptD"
                     /locus_tag="VNU03_07190"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HWP74281.1"
                     /translation="MCGPRRLARLALLLLSALVSIGIGAAPGWGQPASPAGGVTVRAG
                     GEEATVVADQIQQVGGSSDLLIAVGNVEITRGQSRLLADRVELNRDTGQAVAQGKVVF
                     YDGPDRLVGERIDYNLKTGTGVVYNGSAMSAPYYRLSAERMDRVGDSVYEVRRGVFTT
                     CEGDDPAWSFHFASGNADLEDIVYGRDASFWIKSVPVWPWFPVFAAALRRERQSGFLF
                     PEFGQSSRKGFFTRVPYFWAIDDSQDLTVTLDAFSQRGLGVEGQYRYVLSRDQRGDLN
                     VFGVNEVLRNDAHAHVPENRGWASFHHDWQITLSFKVNANVVSDDLIFRDYGNRLPDR
                     ANQRAETNVFVTQVWDAWSLVGNVKWYQDLTTARAIELQRVPDIQLTGIRQPLPGVPG
                     VLYENSASFVNFIREVGPEGMRADFHPRFLMPIPVGGLLTVTPWAGGRLTYYDQRVID
                     QRITRVGGLLVEESVYDPRVRRQVEVGVDVESRISRVYTTDGSGNVAAYQHVIEPRVR
                     LIEIRGLDQKAFPTYDPGASTATGIDPGYERRTGIDRLGKANEVTYYLTNILNAKTVA
                     GPDQAPVRWEMIRFTLSQTFKMDPVSQPLKDLYGDLTIQPNQRIGFHADARYNVYNLG
                     LREANADIRLTYPRVTVGVGPRFNEEGNSRYLHAESVVKVLNNLDVRGATSWDVLKGR
                     AIENRVGIDWRFSCWSVSAEYVNRNSDESEFRVMVNLLGVGQVGSSARAGGF"
     gene            14379..15485
                     /locus_tag="VNU03_07195"
     CDS             14379..15485
                     /locus_tag="VNU03_07195"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWP74282.1"
                     /translation="MSIPQIDLKRQHEALRAELMDASARVLDSCRFVLGPEVQTLERE
                     LAALCGARLGVGTGSGTDALLLALKAVGVVPGDEVITSAFSFVASATTVVMAGATPVF
                     VDVDPATYNLDPAQVERAITPRTRAVVPVHLYGQPAAMDAICALARAHRVAVVGDAAQ
                     AVGAAYGGRPVAAWGDVSILSFYPTKNLGGCGDGGMVLTTRDDVAERVRLLRDHGAPR
                     RYTHVELGGCSRLDELQAALLRVKLKRLPQWNERRRAIAAHYSAALAGLPLGLPIEQA
                     PAHHIYHQYTVRSAKREALAAALAARGVGTSIHYPSTIPAQPVFSRPNADREFPVATQ
                     AAAEVLCLPCFPELTDLEVAEVVEAVKRSVAEVM"
     gene            15487..16809
                     /locus_tag="VNU03_07200"
     CDS             15487..16809
                     /locus_tag="VNU03_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWP74283.1"
                     /translation="MTPLGALGSRLEERLTTHAATVGVVGLGYVGLPLAVGFARAGFR
                     VIGVDADPARSALLASGTSPVEDVPTAELRPLVESGRLVVRSTPEALGEADAIIICVP
                     TPLGKSKEPDISFIVAAAEGVAGVLRPGRLVVLESTTYPGTTDEVLLPRFSRGGLRVG
                     EDFFLAFSPERIDPGNRTWGLSRIPKVVGGVTPACRHLAETLYRQIIDRVVPVSTTQV
                     AEMVKLFENTFRSVNIALVNELAIMCRRLGMPVWEVIAAAATKPFGYMAFQPGPGIGG
                     HCLPSDPHYLSWRSRLAGYEAKFIAFADEINGGMPRYVVELVRDALNEQTRAVKGSRI
                     LALGVAYKAGVGDIRDSPALEIIESLRALGAHVDYADPRVPQITVGGHRLEAVAWEQA
                     DLGAYDLVLLLTAHAEFDPKRLVSDARLVVDTRNATGGLGELPHVVRL"
     gene            16772..17740
                     /locus_tag="VNU03_07205"
     CDS             16772..17740
                     /locus_tag="VNU03_07205"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HWP74284.1"
                     /translation="MASASSRTWSVSERPGRTVVSTLVAAFVALRPRQWVKNFFVFAG
                     LIFSQSLFTPLIWPALGAFLIFCALSGAIYVFNDLADVEKDRLHPTKRHRPIARGALS
                     VPAAAGLGVVLLAGSLVAAYFLSVPFGVVATAYAGLLTAYSVWLKHMVILDVLTVAAG
                     FVLRAVAGAAAIGVEISGWLVICTILIALFLALGKRRHEYLTLKGDAAAHRPILAEYS
                     EGFLDQMVAVVTASTVTAYALYTMSPETVGKFHTHLLPVTLPFVLYGIFRYLYLLYRR
                     EMGGNPSDLLLNDRALLVNTVLWMIALLVIIYGPEWGPAWSARWSR"
     gene            17728..19080
                     /locus_tag="VNU03_07210"
     CDS             17728..19080
                     /locus_tag="VNU03_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP74285.1"
                     /translation="MESLSVEPGEEEIAGWGRHPVGRGRVERPERLALPAGGRPLVPR
                     GLGRAYGDAALPANPGGLVLETTRADRIHSFEPATGRLHCEAGLSLAEILRVFVPRGW
                     FPPVTPGTKFVTVGACVACDVHGKNHHRDGSFGSFVDRVTLCTADGRLVECGPDRERE
                     LFLATLGGMGLTGLIVDVTLRLRRVESPWIVLETQGMASLDAMLEGLRLSATHWPYTV
                     GWIDCLARGADLGRGILMRGRHATQVEAGTRRAPRALPLRVPFDAPEWLLNPLFMRWF
                     NRLYAWSHGTALRRRVVPYDAFFYPLDAVGQWNRLYGRRGFLQYQCVLPRAAGPRPVA
                     ILLERLGVAGAASFLAVIKDCGPASDAYLSFPIEGTTLALDLPNRGAVTEALVHDLNA
                     TVIEHGGRVYLAKDAVTRAEDFARMMPRLAEWRAVRERWDPGHRFRSAQSVRLFGDSA
                     "
     gene            19112..>19408
                     /locus_tag="VNU03_07215"
     CDS             19112..>19408
                     /locus_tag="VNU03_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP74286.1"
                     /translation="MKIAIVGATRGMGRALARRLAERGDALFLLGRDETELGLSARDL
                     EARGAPAPVGQARLDLGESAGFAAALDAADRSLSRTDALVVTGGLFARQDELAAD"
BASE COUNT         2583 a         6988 c         6987 g         2850 t
ORIGIN      
        1 aacagatatt caacgcctcg cgcgacgggg gtgggggggt ggccgggctc ccgcccggga
       61 accgtggctt ctgcgggaag gcgaagcgga ggccggtctg tctcgtggct cgaacgagac
      121 ggcgcggcgc ggtgcgactc agcgtgttcc cgggcgggag cccggccacc ccccaccccc
      181 gtcgcgcgag gcgttgaata tctgttaaac agcggtgtgt cacatctcgc gccgtatgct
      241 cctgcgcgcc gggactaggg cttcgaggtg agggtgtcga gggtggtgcg ggcggtgggg
      301 gagaggtcga ggaagcggag gccggcggtg tccttgcggg accacatgac ctcggcccgg
      361 tccacggtta ccgtgggggc gccgggcccg agctccatct ggagcttcac gatctcgccc
      421 ggctcgagtc gccggggcac cgcgcgcagg gcgatgccgg tccgcgagag gtcgctcgcc
      481 tccgcgacca cggtgagctc gccgtcggag atggagacgc gcccctcctg gcggcggcgc
      541 ggggcggtcc gcgagttgaa gcccgcgggc tcgcggtggg cgtcgtcggg cgccgggccg
      601 agctcgccca gcaccgcgta gatcttcacc gggatcgcct tgcccttgac ggtgacctcg
      661 cccaggtcgc gcaccgcgaa gtgctcgcgg acctcggcgt aggtgccctc gctgatgatg
      721 atcggcacgc tgaactcctt ggtgatgccc tcgagccggg agcccaggtt gatcgtgtcg
      781 ccgatcgcgg tgtactcgag gcgctgctcg gagccgatcg tgcccaccac cgcgtcgccg
      841 gtgtggatgc cgaccccgca ggcgaggtgg ccgccgtgcc gcgccgcgaa gcgctcgccg
      901 aggggccgca cccgctcctg gaactcgagc gcggttcgca ccgcgcgcag cgcgtggtcc
      961 ggcgcctcga ggggcacgtt gtaaagcgcc atgatcgcgt cgccgatgta cttgtcgacg
     1021 gtgcccccgt gcttgaacac cgcctcggtc atcacggtca ggtactcgcg caggaaggtg
     1081 acgacctctt ccggctgcat cttctccgac atggacgtga agccgcggat gtcggagaag
     1141 agcacggtca cgcgacgccg ggtggcggcc agcgtactgt cgtccttgtg ccggatgatc
     1201 tcggccacca cgtccggcga gaagaagcgc gagaggcggc gcttctcccg ctgctcctgg
     1261 gtgaagttct tggccaccgt gcccgcgtag gtggcgagca gcgcgagcgg caccggcacc
     1321 acctcgaccc agtagcgggc ccacaggaag gcggcgtggc tggccgcgag gtagccgccg
     1381 atcgcggcgg ccacgatccc gaaggcgggc aggggacgca gcgcggtggc ggtccacgcc
     1441 gcgcccgccg ccgcgagcac cgccaccacc ggcacgagca gcctcgaggc ccggcgcagg
     1501 ggaatgccct gcagcagggt ctccagggtg tgggcgtgga tctcgacgcc cggcatgccg
     1561 gactgagagg cgaacggggt ggggaagatg tcgtgcagcg tgggtgtcgt cgcgcccacc
     1621 aggacgatct tccccgcgaa gacctcgggc gggacctcgc cggtgacgat ccggtggtag
     1681 gcgaccatgg ggaacgtgcc ggggccgccg cgatagttga cgaggagctc gtgccccgag
     1741 ggggcggggg cccgggtcag cccggccttc tcggcgaggc ggtagatcag catgtcgaag
     1801 ccgagccagt cgcggtcctg gaaatcgcgg cggagggtgg ctcggcggac gaacgcgtcg
     1861 gcgtccgagt cgtagtcggc cgagccgaag gcggccttgc cgcggatctc cgggatcggc
     1921 ggattcagcg cctgcttcac gaaggaggcg tcgccgacgg tggtgagcgc ggcggccagg
     1981 acgatccgat cccggattcg atcgaccgcg gccccgagcg ccacatcgtc ggacgggccg
     2041 tgcaacgagg gctcggtgaa caggatatcc atgccgatcg cggccgggtt cccgcgcccc
     2101 accaggtcca ggaacttggc gtgcaccgcg cggggccagg gccaggcgag attcagcccg
     2161 tcgaagtcgt cctcgtcgat ggtgacgacc acgatgggcg acgcgggggt cttcgggccg
     2221 cggatcgaga actgggcgtc cagggccttc agctcggcga tctcgaagaa gcgcagaggg
     2281 ttgacggtgg cgagcaccac cacggccagg ccggccaggc tgccggcgag gagcacgcgc
     2341 caggaggcgg tgagggatcg caccgacgtc aagcgtagca caagggaagt tgccgccgcc
     2401 gcggggaaac gtctacactc gtgtcatgcg ggttcgctcg atcctgctcg ccgcgttctg
     2461 ggtcgccatg ttcatctggg tcggctacaa cggcatgcag gcgatctcct cctacttcca
     2521 gaccaacgac atcgccgagc aggcgttccg ggacgcctcc gacaagcagc gccagcgcaa
     2581 cccgggcgag ctggtctcct ccgatttcct cgccgatctc cggaccgggg tgctggccgg
     2641 cgcgcgccgg cagggcgtgc agctggaccc gtcgaccctc aaggtctccg cggagggcgc
     2701 cctcgtccgg gtcaacctcg ggtggaccta tcgcacctgg ccgctgacca tgggcggctt
     2761 cgacaccgcc ctgccggtgc cgatgtggct cggccggagc ttcgatccgc agctcggcat
     2821 gcgccgcctg ttctgacccg gccgggggca tgagcgagcg catcatcatc gcggaggacg
     2881 acgaggatct cgccttcgtc ctccgcgagg cgctgcggcg ccagcgctac gacgtcgagg
     2941 tcgcgcccac cgcgggcggt ctgctcgacg ggctcaaggc cgctccgtac gacctgatcc
     3001 tcctcgacgt gcgcctgccc gacatggacg gactcgacgc gatcccgcgc tgtcgcgagc
     3061 tggcgccgga cacgcccatc atcgtgatga ccgcgcacgg gacgcgacag accgcgatgg
     3121 acgccatcac gcgcggggcc tacgacttct tcaccaagcc gctcaagatg gacgagttcc
     3181 aggtggtcgt cgcgcgcgcg ctcgaccgcc ggcgtctgca gaagcaggtc aaggcgctgg
     3241 gcgacagcca gccgagcggg ctcgatgggc tgatcggcca gagcgaggcg ctcgagcggg
     3301 tggtggacat ggcccaccgc gcggcgccca ccgacctgac catcctcgtc cagggcgaga
     3361 gcggcaccgg caaggaagtg ctggcccgcg cgatccaccg gttgagcgcc cgcaaggacg
     3421 gcccgttcgt gccggtcaac tgcgcggcga tcccggaggg cctcctcgag tcggagctgt
     3481 tcggccacga gcggggcgcc ttcaccggcg cggtgcgggc ccgtcccggc cgcttcgagc
     3541 tggccaagga gggtaccctc ttcctcgacg agatcggcga catgccgctg tccatgcagg
     3601 ccaagatcct gcgcgtcctt caggagcgcg agttcgagcg ggtgggcggc acgcgcacca
     3661 tgaccgcgga cgtgcgggtg atcgcggcca cccaccgcga cctcgatgcg gcggtggccc
     3721 agggcacctt ccggcaggac ctgttctacc ggctccaggg cgtcgggatc gtgctcccgc
     3781 ccctccggga gcgcgtcgac gacctgccgc tgctggccac ccacctgctg gagcgggcgg
     3841 cccagcgcct cacccgccag cccgcggtgc tctcgcccga ggccctgcgc tgcctctgga
     3901 cctactcgtg gcccggcaac gtgcgcgagc tccagcacgt gctcgagggg gccatggtgc
     3961 tctccgacgg ggtgatccgc cccgatcacc tcccccccgc gatccagcgc gcggtccagg
     4021 cgcggcccgc cgcggaggcc ggtccggtac tgtcgggctc gctcgacgag gcgctcgagg
     4081 agtgggagcg gcgcatgatc ctggacgcgc tccagcagac ccagggcgtg caggcgcgcg
     4141 cggccaagat cctcggggtc tcggagcgca gcctctggta ccggatcaag aagctcggca
     4201 tccaggtgcg cacgccggac gacgcgccgt cggcctgacc cggtttcatt tccgattccc
     4261 ctcgtgtatt gtcaggttcg atgaaagtcc gcaaggccgt gttccccgcc gccggtctcg
     4321 gcactcgctt cctgcccgcc accaaggccc agcccaagga gatgctcccg ctggtggaca
     4381 agcccaccat ccagtacgtg gtggaggagg cggtggcgtc cggtctcgag gagatcatcc
     4441 tggtcaccgg gcggaacaag cgcgcgatcg aggaccactt cgacgcggcg ttcgagctcg
     4501 agtactacct gcaggatcgc ggcaagatgg aagagctggc ccagatcaag acgatctccg
     4561 agctggcctc cgtctcctac gtccgccaga aggagccgct cgggctcggg cacgcgatcc
     4621 tctgtgccaa gccgctggtg ggcgacgagc cgttcggggt gttcctgggc gacgacatca
     4681 tcgtgtcgcg ggtgccgtgc atgcggcagc tcatcgacgt gttcgagcgc cacggcgggc
     4741 cggtcctggc cgtgatgcgg atgccgcgcg aggagatcgg ccgctacggc gtcatcgcgg
     4801 cgcgcggcct gggcgccaat gtctacgagg tgcaggacct ggtcgagaag ccggaccccc
     4861 gtgacgcccc gtcggacctg gccatcatcg ggcgctacgt gctcaccccg gatctcttcg
     4921 gcatcctgaa ggagaccgcg ccggattcgc ggggagagat ccagctcacc aacgggctcc
     4981 gcgtgctccg cgggcggcgg ccgatctacg cggtcgagtt cgagggcaag cgccacgaca
     5041 ccggcgagaa gctgggcttc ctcaaggcca ccgtcgagtt cgccctggcc cggccggacc
     5101 tctccgacgc cttccgcgag tacctgagga gcctgaagct gtaaggacga ggcagatatg
     5161 tcttgtgggg cgctactttg cccctcgtca tcccgcgtcc attgattgac ttcccccggg
     5221 gattctggga caatccctgc actttatttc agggccggga ggtgtcggca cctatatgaa
     5281 gagccagccc aagcgcacct tccacacggc ctccgagccc gagatcaagg ccggtgaggt
     5341 ctcggacgtc tacttcgatc gcaccgtcga gatccttcgt gccaagggtc tcaacaagca
     5401 cgtcaaggcc gagatccggc tgaagagctt cccgcagccc gactggacgt tcggagtcct
     5461 ggccggcatc gaggaggcct cgggcctgct cgagggcctg ccggtcaacg tctgggccat
     5521 ggacgagggc acgctcttcg gccccgagga cacggtgctc ctcatcgagg gcatctaccc
     5581 cgagtgggcg aagtacgaga ccgcgctgct cggcttcctc tgccaggcct ccggcatcgc
     5641 caccaaggcg gcccggtgca agcgcgcggc cggcccgcgc caggtcatct ccttcggggc
     5701 gcggcgcatg cacccggcgc tcgcgcccat gatcgagcgc aacgccttcg tcggtggctg
     5761 cgacggggtg gcggttacca agtcggccga gctgatcgac gccgatccca ccggcaccat
     5821 cccgcacgcc ctggtcctcc tcttcggcga cacggtggag gcgctccgcg ccttccacga
     5881 ggtggtcgac gccaaggtgc gccgggtggc cctgatcgac accctgcagg acgagaagtt
     5941 cgaggcgatc cgggtggcgg aagcgctcgg tcgagagctc tacgcggtgc gcctcgacac
     6001 gccctcctcg cgccgcggcg acttcttcaa gatcctcgag gaggtccgct gggagctcga
     6061 cctgcgcggc ttcgactacg tgaagatcct ggcctccggc gggatcgacg agtacgagat
     6121 cctcaagctc aaccccctcg tggacggcta cggcgtcggc acctcgatcg cgaacgcccc
     6181 ggtgctctcg tttgcgctcg acatcatgga gatcgagggc gtgccgatgg ccaagcgcgg
     6241 caagcgctcg ggggccaagc aggtgtggcg cttcccgggc accgtccaga acaccgtcct
     6301 ccgtgccggc gcccccgcgc cggtcgggcc cgacggccgg gccgcggagg cgctgctgaa
     6361 gccgctgatc gcgggcggcc ggctggtgcg cgacctgccg cccccgcgca ccctgcgcga
     6421 gtacgtgatg gagcagatgg cgcgcattga catcacggcg gggcggaatc ccgggctgcg
     6481 cggcgactac tagccgaatg ggcgtcctgg atgagatcgt cgcccacaag cgcgccgagg
     6541 tggccgagct caaggcccgg cggccgatgg cgacggtggt gggagcctgc cgcggcctcg
     6601 cgtccgcccg cgacttcgag gcggccctcc ggcctgggcc cggcgcgcga gttcgcctga
     6661 tcgccgaggt caagcgcgcc tcgccctccc agggcctctt ccgcgccgat ctcgacccga
     6721 tcgcccaggc cgcgacctac gccgccgcgg gcgccgccgc ggtctcggtg ctgaccgacg
     6781 cgcgctactt ccacgggagc ctcgacgacc tggtccaggt gcgcggtgcg gtcccggtac
     6841 cggtgctccg caaggaattc atcgtcgacg agtaccagct ctgggaggcc cgcgaggcgg
     6901 gcgcggacgc ggtgctgctg atcgtggccg cgctcgagga cgcggcgctg cgcgacctgc
     6961 tctcggcggc caagggcgtg ggactggcca ccctggtgga ggtgcacacc gcgggcgagc
     7021 tggatcgcgc gctgcgcctg ggcgctccgg tgatcggggt caacaatcgc gatctgcaga
     7081 ccctgaccac gaccctcgcc ccgtcgctgg ccctcctgcc gcagatcccg ctcgggccca
     7141 tcgcggtgag cgagagcggg ctccgcaccg gagccgacgt ggcgcaggtg gtggcggccg
     7201 gcgcccacgc ggtgctggtg ggcgagacgc tcctgcgggc ggacgacgtg gccgcgaagg
     7261 tgcgcgagct gtcgcttcga tgaggatcaa gatctgcggc atcaccaatg tcgacgacgc
     7321 gcggctctgc gtggaggcgg gggccgacgc cctcggattc atcttcgtcc cgaacacgcc
     7381 ccgctgcgtg accacggaga ccgccgccct catcatcgcc gcgctgccgc cgttcgtgac
     7441 gccggtgggc gtcttctggg accacgcgcc cgggcaggtg aaggcggtgg ccgaggagtg
     7501 ccggctcggc gcgctgcagt tccacggcga cgaggcgccc gaggatctgc tcgagcatcg
     7561 cctgccggtg atcaagacct tgaaggtcgc cggcgccgcg gacctggccc ggatggccga
     7621 ctaccgcgtc gccgcgttcc tgctcgactc gccgtcgcgg tggagcgagg gcgagtcccg
     7681 gccgccgatt ccctgggagg tggccgccgc ggcggcgcgc cggcacccgg tgctgctctc
     7741 cgccgggctc accccggaca acgtgagcct cgcggtccgg cgcgtccgac cctacggggt
     7801 ggacgtcaac tccggggtgg aggcccgtcc gggccgcaag gatccggtcc gcgtgcgacg
     7861 cttcgtgacc gaggcgcgcg cggcggcgga gcagccgtga gcgcgacccg cgtgcggccc
     7921 gccgcgctgc cggacgcggg cggtcacttc ggtcgcttcg gcggccgctt cgtgcccgag
     7981 accctcatgc agccgctggt cgatctcgag aaggcctacc gggccgcgcg cggcgacgca
     8041 cgcttccgcc ggcgcctgcg cgagctgctc tcgacctacg cgggccgacc caccccgctc
     8101 tacttcgcgg agcgcctgac ccgccacgtg ggcggcgcgc gcatctacct caagcgcgag
     8161 gacctctgcc acaccggcgc tcacaagatc aacaacgtgc tgggccaggc cctgctcgcg
     8221 cagcggatgg gcaagccgcg cgtggtcgcg gagaccggcg cgggccagca cggggtggcc
     8281 tccgccaccg cggcggccct tttcggactc gagtgccagg tctacatggg ccgggtcgac
     8341 gtggagcgcc aggccctcaa cgtcttccgc atgaagctgc tcggcgcgac ggtgatcccg
     8401 gtcgagagcg gctcccggac gctcaaggac gcggtcaacg aggcgctgcg cgactgggtc
     8461 accaacgtgc gcaccaccta ctacctcctc ggctcggtga tgggtccgca tccgtacccg
     8521 atgatggtgc gcgacttcca ccgggtgatc ggcgaggaga cgcgggccca ggcccgccgc
     8581 gcgaccggcc ggctcccgga tctcctggtg gcctgcgtgg gcggcggctc caacgcgatc
     8641 ggtctcttct ggccgttcat cggcgaccgg cgggtgcgca tcgtgggcgt cgagcccggc
     8701 ggccacggca tcaagagcgg caagcacggc gcctcgctcg gctcgggcgc ggtcggcgtg
     8761 ctgcacggca gcatgagcta cgtgctccag aacgacgacg ggcaggtggc ggaggcgcac
     8821 tcgatctcgg ccgggctcga ctatcccggg gtggggcccg agcactctta ctacaaggac
     8881 accggccgct tcgagtacgt ctcggtgacc gacgccgagg cgctcgaggg gttccaggtt
     8941 ctcacgcggc tcgagggcat catgcccgcg ctcgagtccg cccacgcggt cgcgcacgcg
     9001 atgcgggccg ccgcgcggat gaagaagacg cagacggtgg tggtgggcct gtcgggccgc
     9061 ggcgacaagg acgtgcacac cgtcggcgag gccctcgatg cccggccggg caacgggctc
     9121 gggggcaacg gccacggagg cgcccggtga aggcggcgac cggccgcgcg ccctcgcgcc
     9181 tcgacgccac cttcgcggcg ctccgcgacc gtcacgagcg cgccctcgtc gcctacttca
     9241 cggccggcga cccgtcgctc gcgctcaccc gcaagctggt ggtggaggcg gcccgccgcg
     9301 gcgcggacgt catcgagctc ggcatcccgt tctcggatcc gctcgcggac ggaccggtga
     9361 tccagcgggc tacccagcgc gcgctggcgg ccggggtcac cctgccgagg gtcctcgagc
     9421 tggtgcggga gatgcggggc gaggtgtcgg tgccgctcgt cttcctcacg tactacaacc
     9481 cgatcctggc cttcgggctc aaggccttct gccagacctc ggtggaggcg ggcatcgacg
     9541 gggtgatcgt cgcggacctg ccgcccgagg agtcggggcc cctgcggtcg gaggcgatgg
     9601 cggccgggct cgatctcgtc cacctggtgg cgccgacctc gacacccgag cgcatgcgca
     9661 agatcgcgcg ggcgagcgag ggcttcatct atctggtcgc gctcaccggc gtcaccgggg
     9721 agcgcaccgc gctcgccccc gagctggccc agcaggtgcg cgccctgcgc gcgatcacca
     9781 ccaagccggt gtgcgtgggc ttcggcatcg gcacgcccga gcaggccgcc ctggtgggcc
     9841 agctggccga cggggtcgtc gtgggctcgg ccatcgtgcg cctggtggag cagcacgtgg
     9901 gctcgcccga gctgctggcc cgggtgggcg atttcatcgc ggggctgaag gccccgctgc
     9961 ggggcgggag ctagcgcgtg gcctggttct ggaagaagaa ggaggacggc gacgaggacc
    10021 ggccgaagaa ggtcgtgatc gccgagggcc tgtgggtcaa gtgcgactcc tgccgcgaga
    10081 tcgtctaccg cgcggaggtc gagcgcgcgg gccgcgtgtg ccccaagtgc ggctacccct
    10141 tccgcatctc ggcccgcgag cgcatcgcct cgatcgtcga cgagggctcc ttcgaggagc
    10201 gcgagggggg cctgcgctcc aaggatccgc tcagcttcaa ggacaccaag cgctactcgg
    10261 accggctcaa ggccgcgcgg gccaagtcct cgatggagga ggcggtgatc accgggaccg
    10321 cccggatcgg cggctacccg gtcgcgctcg cggtgttcga gttcgcgttc ctgggcggca
    10381 gcatggcctc ggtggtcggc gagaagctca cccgcaccat cgagctcgcg ctgcagaagc
    10441 gcgtgccgct cctgatcgtc tccgcctccg gcggagcgcg catgcaggaa ggcatcctct
    10501 ccctcatgca gatggccaag acctccgccg cgctcgcacg cctggccgcc gagcgggtgc
    10561 cctacatctc gctgctcacc gatcccacca ccggcggcgt caccgcgagc ttcgcgatgc
    10621 tgggcgacct gatcctggcc gagccccgcg ccctcatcgg tttcgcgggg ccccgcgtca
    10681 tcgcggagac gatccggcag ccgctgcccg acggcttcca gcgctcggag ttcctgctcg
    10741 agcacggcca gctcgacctg gtcgtggagc ggcgggagct gcgggccacg ctccggagga
    10801 tcctcgcctt cttcggcgga cgcgaccccg cgcccgcccc atgacctacc cggaggccgt
    10861 ggcccggatc ctcggcctcc gcggcgggga gcgggtcggc atgcgccccg gcctcgagcg
    10921 catcgaggcg ctgctcgacg ccatcggccg tccggagcgg gcgttcacca tcgcccaggt
    10981 cggcggcacc aacggcaagg gctccatctc ggcgatgctg gccgccatcc tccaggcgtc
    11041 ggggcggcgc gtcggcctct acacctcgcc gcatctccgg cactaccgcg agcggatccg
    11101 ggtggacggc cagcccatct cggaggccga cttcgtggac ggggtggacg cgctcggcac
    11161 gctcatcgcg cggctcgacg tcaccgtctt cgaggccggc accgcgctgg ccctcgacca
    11221 cttctgccgc tcgcgcgtcg acgtggcggt gctcgaggtg ggcctgggcg gccggctcga
    11281 cgccaccacg gtgggccggc cggcggtggt ggtgctcggg cccatcgact acgaccacca
    11341 gctcgagctg ggcgacaccc tgaccgccat cgcgggcgag aaggcggcca tcatccggag
    11401 cggggtggcc ttctcggccc ggcaggaccc ggaggcggcc cgggttctcg aggagcgggc
    11461 cgcccgggtc ggcgtgccgc tcgtgcgcga gggccacgag ctgcacgtga cgccgcgcgg
    11521 cttcaccctc gaggcgcagc ggctcgacct cgccggtccg gactggcacc tcgcggacgt
    11581 ggcgtgtggg ctcctgggcg tcttccagcc gggtaacgcg ctactggccg ccgccgcggg
    11641 ccgcgcgctg ggtgccgacg agcccgcgat ccgggccggc ctccgcggcg cgcgctggcc
    11701 ggggcgcttc caggtctttc gccgcgaccc ggtcgtgatc ctggacggcg cgcacaatcc
    11761 ggccggggct cgggccctcg ccgcgtcgct gcgcgcctac ttcccgggcc ggccggtcac
    11821 cttcgtgatc gggatcctcg cggacaagga cgcggccggg atcctcggcg ccctgtcgcc
    11881 cctggcggcc ggcgtcgtgc tgaccacctc cgggaacccg cggtccgccg ccccggcgtc
    11941 gctccgcgcg ctcctgcccg cggacctgcg ggtggaggtg gcgccgtccc cgtcggaggc
    12001 gctcgcgcgg gccaccgccg gggcggccgg cgggctcgtg tgcgtggccg gctcgctctc
    12061 gctgatcggc gacgtcctgg ccgccgccgg cgacgggcag gatgtccttt tcaccgcggg
    12121 ggcccgctgt tagcatgggc actcatgccg ccggtctcgg ctcctgctcg ctcgatgtgc
    12181 ggccctaggc ggctcgcgcg actcgccctt ctcctcctct cggccctcgt ctcgatcggc
    12241 atcggcgccg cgccggggtg ggggcagccc gcgagccccg cgggcggcgt cacggtccgc
    12301 gcgggcggcg aggaggcgac cgtggtcgcc gatcagatcc agcaggtggg cggcagctcg
    12361 gacctgctga tcgcggtcgg caacgtcgag atcacgcggg gccagagccg gctcctcgcc
    12421 gaccgggtcg agctcaaccg cgacaccggc caggcggtgg cgcagggcaa ggtggtgttc
    12481 tacgacgggc cggaccgtct cgtcggtgag cgcatcgact acaacctgaa gaccggcacc
    12541 ggggtggtct acaacggctc cgcgatgtcc gcgccgtact accgcctctc cgcggagcgg
    12601 atggaccggg tcggcgacag cgtctacgag gtgcggcgcg gcgtcttcac cacgtgcgag
    12661 ggcgacgatc ccgcctggtc gttccacttt gcctccggca acgccgacct ggaggacatc
    12721 gtctacggcc gcgacgcctc gttctggatc aagagcgtgc cggtgtggcc gtggtttccc
    12781 gtcttcgcgg ccgcgctccg gcgcgagcgc cagtccggct tcctcttccc ggagttcggg
    12841 cagtcctcgc gcaagggctt cttcacgcgg gtgccgtact tctgggcgat cgacgacagc
    12901 caggacctca ccgtcacgct cgacgccttc agccagcggg gcctcggcgt ggagggacag
    12961 taccgctacg ttctgtcgcg cgaccagcgg ggcgatctca acgtgttcgg ggtgaacgag
    13021 gtgctgcgca acgacgcgca cgcccacgtg cccgagaacc gcgggtgggc gtcgttccac
    13081 cacgactggc agatcaccct ttcgttcaag gtcaacgcca acgtggtcag cgacgacctg
    13141 atctttcgcg actacggcaa ccggctcccc gaccgggcga accagcgggc ggagaccaac
    13201 gtcttcgtca cccaggtgtg ggacgcctgg agcctcgtcg gcaacgtgaa gtggtaccag
    13261 gacctcacga ccgcgcgggc gatcgagctg cagcgggtgc cggacatcca gctcaccggt
    13321 attcgccagc ccctccccgg cgtgccgggg gtgctgtacg agaactcggc gagcttcgtc
    13381 aacttcatcc gcgaggtcgg acccgagggc atgcgggccg acttccatcc ccgcttcctg
    13441 atgccgatcc cggtgggcgg cctgctcacg gtgaccccct gggcgggcgg ccggctgacc
    13501 tactacgacc agcgcgtgat cgaccagcgg atcacgcgag tgggcgggct cctcgtggag
    13561 gagtcggtgt acgatccgcg cgtccggcgc caggtcgagg tgggcgtcga cgtcgagagc
    13621 cggatctccc gcgtctacac caccgacggc agcgggaacg tcgccgccta tcagcacgtg
    13681 atcgagcccc gggtgcggct catcgagatc cggggcctgg atcagaaggc gtttcccacc
    13741 tacgacccgg gcgccagcac cgcgaccggg atcgatcccg gctacgagcg gcgcaccggg
    13801 atcgaccggc tgggcaaggc caacgaggtg acctactacc tcaccaacat cctgaacgcc
    13861 aagacggtcg ccggtcccga ccaggctccc gtccggtggg agatgatccg cttcacgctc
    13921 agccagacct tcaagatgga cccggtctcc cagcccctca aggacctgta cggcgacctc
    13981 accatccagc ccaaccaaag gatcggcttc cacgccgacg cccggtacaa tgtgtacaac
    14041 ctcgggctgc gtgaggccaa cgcggacatc cgcctgacct acccgcgggt gaccgtcgga
    14101 gtcggcccgc gtttcaacga ggagggcaac tctcggtacc tgcacgcgga gtccgtggtc
    14161 aaggtgctga acaatctcga cgtccgcggg gccacctcct gggacgtcct gaaggggcga
    14221 gccatcgaga atcgcgtcgg catcgactgg cgcttcagct gctggtccgt cagcgccgag
    14281 tacgtcaacc ggaacagcga cgagagcgaa ttccgggtca tggtgaatct gctgggcgtg
    14341 ggccaggtgg gctcctcggc ccgggccggc ggcttctgat gtcgatcccc cagatcgacc
    14401 tcaagcgcca gcacgaggcc ctgcgcgccg agctgatgga cgcctcggcc cgggtgctgg
    14461 actcctgccg cttcgtcctc gggcccgagg tccagaccct cgagcgcgag ctcgccgccc
    14521 tgtgcggcgc gcgcctcggg gtgggcaccg gctccggcac cgacgcgctg ctgctggcgc
    14581 tcaaggcggt gggcgtcgtg ccgggcgacg aggtcatcac ctccgcgttc tccttcgtgg
    14641 cctccgccac caccgtggtc atggcgggag cgacgccggt cttcgtcgac gtcgatcccg
    14701 ccacctacaa tctcgacccc gcgcaggtgg agcgcgcgat caccccgcgc acgcgggccg
    14761 tcgtcccggt gcacctctac ggtcagcccg ccgcgatgga cgcgatctgt gccctcgcgc
    14821 gggcccaccg ggtggcggtg gtgggcgacg cggcccaggc ggtgggcgcg gcctacggcg
    14881 gccggccggt ggccgcgtgg ggcgacgtct cgatcctctc cttctatccc accaagaacc
    14941 tgggcggctg cggcgacggc ggcatggtgc tgaccacgcg cgacgacgtg gccgagcggg
    15001 tgcggctgct ccgcgatcac ggcgcgcccc gccgctacac ccacgtcgag ctgggcggct
    15061 gcagccgcct cgacgagctg caggccgcgc tgctgcgggt caagctcaag cgcctgccgc
    15121 agtggaacga gcgccggcgc gccatcgcgg cccactactc ggccgcgctc gccggcctgc
    15181 ccctcgggct gccgatcgag caggcgcccg cgcaccacat ctaccaccag tacacggtgc
    15241 gctccgccaa gcgggaggcg ctcgcggcgg ccctggccgc gcgcggcgtc gggacctcga
    15301 tccactatcc gagcacgatc ccggcccagc cggtcttctc gcggcccaac gcggatcgcg
    15361 agttcccggt ggcgacgcag gccgccgcgg aggtgctctg cctgccctgc ttcccggagc
    15421 tgaccgacct cgaggtggcc gaggtggtgg aggcggtgaa gcggtccgtc gccgaggtga
    15481 tgtgaggtga cgccgctcgg cgcactcggg tcccggctcg aggagcgcct gacgacacac
    15541 gccgcgacgg tgggggtggt gggcctcggc tacgtcggcc ttccgctcgc ggtcggcttc
    15601 gcgcgggccg gcttccgggt gatcggcgtc gacgccgatc cggcccgcag cgcgctgctg
    15661 gcgtccggga ccagcccggt ggaggacgtg ccgaccgccg agctgcgccc gctggtggag
    15721 agcgggcgcc tcgtggtgcg ctcgactccc gaggccctcg gcgaggccga cgccatcatc
    15781 atctgcgtgc ccaccccgct cgggaagtcg aaggagcccg acatctcctt catcgtggcc
    15841 gccgccgagg gggtggccgg ggtgctgcgg ccgggccggc tcgtggtgct ggagtccacc
    15901 acctatcccg gcaccaccga cgaggtgctc ctgccgcgct tcagccgcgg cggcctgcgg
    15961 gtgggggagg acttcttcct cgccttctcc cccgagcgga tcgatcccgg caaccgcacc
    16021 tgggggctct cgcgcattcc caaggtggtg ggcggggtca cccccgcgtg ccgccacctc
    16081 gccgagacgc tctaccggca gatcatcgac cgggtggtcc cggtgtcgac gacgcaggtc
    16141 gcggagatgg tgaagctctt cgagaacacc ttccgcagcg tgaacatcgc gttggtcaac
    16201 gagctggcca tcatgtgccg ccggctcggc atgccggtgt gggaggtcat cgccgccgcc
    16261 gcgaccaagc ccttcggcta catggcgttc cagccggggc cgggcatcgg cggccactgc
    16321 ctgccgagcg atccgcacta tctctcctgg cgtagccggc tggccgggta cgaggccaag
    16381 ttcatcgcct tcgcggacga gatcaacggg ggcatgccgc gctacgtggt ggagctggtg
    16441 cgggacgcgc tcaacgagca gacccgcgca gtgaagggat cgcggatcct cgccctcggc
    16501 gtcgcctaca aggccggggt cggcgatatc cgcgactcgc ccgcgctcga gatcatcgag
    16561 tcgctccggg ccctcggagc ccacgtcgac tacgcggatc cgcgggtgcc gcagatcacg
    16621 gtgggtggcc accggctcga ggcggtggcg tgggagcagg cggacctcgg cgcctacgac
    16681 ctggtgctgc tcttgaccgc ccacgcggag ttcgacccga agcgcctggt ctccgacgcg
    16741 cggctcgtgg tggatacccg caacgccacc ggtggcctcg gcgagctccc gcacgtggtc
    16801 cgtctctgag cggcccggcc ggacggtcgt gagcaccctc gtcgctgcct tcgtcgcgct
    16861 gcggccccgg cagtgggtca agaacttctt cgtcttcgcg ggcctgatct tctcgcagag
    16921 cctgttcacc ccgctgatct ggcccgccct gggcgccttc ctgatcttct gcgcgctctc
    16981 cggcgcgatc tacgtcttca acgacctggc cgacgtcgag aaggaccgtc tgcacccgac
    17041 caagcgccat cgtccgatcg cgcggggcgc gctctcggtg cccgcggccg cggggctggg
    17101 cgtcgtgctg ctggcgggca gcctggtcgc ggcctacttc ctctcggtgc ccttcggggt
    17161 ggtggccacc gcctacgcgg ggctgctcac cgcctactcg gtctggctca agcacatggt
    17221 gatcctcgac gtgctcacgg tggccgcggg cttcgtgctg cgcgcggtgg cgggggccgc
    17281 cgcgatcggg gtcgagatct cgggctggct ggtgatctgc acgatcctga tcgcgctgtt
    17341 cctggccctc ggcaagcgcc gccacgagta cctcaccctg aagggggacg cggcggccca
    17401 ccggccgatc ctggccgagt acagcgaggg cttcctcgac cagatggtgg cggtggtgac
    17461 cgcctccacc gtcaccgcgt acgcgctcta caccatgtcg cccgagacgg tgggcaagtt
    17521 ccacacccac ctgctcccgg tgaccctgcc gttcgtgctc tacggcatct tccgctacct
    17581 ctacctgctc taccgccgcg agatgggcgg caacccgtcc gatctactgc tcaacgatcg
    17641 cgcgctgctg gtcaacaccg tgctctggat gatcgcgctg ctcgtgatca tctacgggcc
    17701 ggagtggggg cccgcgtgga gcgcccggtg gagtcgctga gcgtggagcc cggggaggag
    17761 gagatcgcgg gctgggggcg ccatccggtg gggcgcggcc gggtggagcg cccggagcgc
    17821 ctggcgctgc ccgcgggcgg tcggcccctg gtgccccgcg ggctcggtcg cgcctacggc
    17881 gacgcggcgc tgcccgcgaa cccgggcggg ctcgtgctcg agaccacgcg cgccgatcgc
    17941 atccactcgt tcgagcccgc gaccgggcgc ctgcactgcg aggcggggct gagcctcgcc
    18001 gagatcctgc gcgtcttcgt gccgcgcgga tggttcccgc cggtcacgcc gggcaccaag
    18061 ttcgtcaccg tcggcgcgtg cgtggcctgc gacgtccacg gcaagaacca tcaccgcgac
    18121 ggatcgttcg ggagcttcgt cgatcgcgtc acgctctgca ccgcggacgg gcgcctcgtc
    18181 gagtgcggcc ccgaccggga gcgcgagctg ttcctggcca cgctcggtgg catggggctc
    18241 accgggctca tcgtggacgt caccctgcgg ctgcgacggg tggagagccc gtggatcgtg
    18301 ctggagaccc aggggatggc gagcctcgac gcgatgctgg agggcctgcg gctgagcgcg
    18361 acccactggc cctacacggt gggctggatc gactgcctcg cccggggcgc cgacctcgga
    18421 cgcggcatcc tcatgcgcgg ccgccacgcc acccaggtcg aggccggcac ccggcgggcc
    18481 ccccgcgcgc tgccgctccg ggtgccgttc gacgcgccgg agtggctgtt gaacccgctc
    18541 ttcatgcgct ggttcaaccg gctctacgcg tggagccacg ggaccgcgct ccggcgccgc
    18601 gtggtcccgt acgacgcgtt cttctacccg ctcgacgcgg tcgggcagtg gaaccgcctg
    18661 tacggccgcc ggggcttcct ccagtaccag tgcgtgctac cgcgcgcggc ggggccccgg
    18721 ccggtggcga tcctgctcga gcggctcggg gtggcggggg ccgcctcgtt cctggccgtg
    18781 atcaaggact gcggccccgc ctccgacgcc tatctgtcgt ttcccatcga gggcacgacg
    18841 ctcgcgctcg acctgcccaa ccgcggcgcg gtgaccgagg cgctggtcca cgacctcaac
    18901 gcgaccgtca tcgagcacgg cgggcgcgtc tacctcgcca aggacgcggt gacgcgggcc
    18961 gaggacttcg cgcggatgat gccccgcctg gccgagtggc gggcggtgcg ggagcgctgg
    19021 gatcccggcc accgcttccg ctccgcccag tcggtgcggc tcttcggaga ctccgcgtga
    19081 aggttgcccc aagcatgaag attgcgcccg catgaaaatt gcgatcgtcg gggcgacccg
    19141 cggcatgggg cgcgcgctcg cccgccggct cgccgagcgc ggcgacgcgc tcttcctgct
    19201 gggccgcgac gagaccgagt tgggcctctc cgcgcgcgac ctggaggccc gcggcgcccc
    19261 cgcgccggtg ggtcaggcgc ggctcgacct cggcgagtcc gccggcttcg cggcggcgct
    19321 cgacgcggca gatcgctcgc tctcgcgcac cgacgcgctc gtggtcaccg gcggcctctt
    19381 cgcgcgccag gacgagctgg ccgccgat
//
LOCUS       DATMCV010000309         5631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_679022, whole genome shotgun sequence.
ACCESSION   DATMCV010000309 DATMCV010000000
VERSION     DATMCV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679022"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1691
                     /locus_tag="VNU03_07220"
     CDS             30..1691
                     /locus_tag="VNU03_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP74287.1"
                     /translation="MLPPGEGNPFLRATVGDMLATQAARVGDREAIVATDRRISYAEL
                     YRDAQRLARGLLACGVRKDDKVALWMPGRPEWLAVQHGCALIGAVLVALNTRYKAHEL
                     RYILRQSDATTLVCADHSGPVDYLETLAEVLPDLSASVPGELDVAEFPALRRVIVHAD
                     DPYPGCLAWRDIEELGDAPETAAALDAARATVAPDDPFTILYTSGTTSFPKGAVISHR
                     NCLPHGWWSGAVMRLTEADRVLHALPLSGTWGGLCIPLATFTHGATLVLMETFEAAVA
                     LRLMEDEGITVWNGVDAMAVPMLEHPDLARRDRSRLRTGGFAATGGGVQGLFEAVVER
                     LGVRLAFQPYGMTEVNAMALVHDLDESPESLAVAGVWPADGLEARVVDPETGRDQPID
                     GEGELWLRGRLVTAGYYQKPEETARAFSADGWFKTGDLAVRHADGRVVFRGRLREVLR
                     ISHFMVAPGEIEAYLQTHPKVLQAFVIGVPDPRTNEAAVAYVIPRPGCADLTEEEVIA
                     HCRGRIASFKVPRHVRVVADVPRTPGPHGDKVQKARLREMFLGGR"
     gene            complement(1695..3143)
                     /locus_tag="VNU03_07225"
     CDS             complement(1695..3143)
                     /locus_tag="VNU03_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWP74288.1"
                     /translation="MHAAGIGLVVLLGVGCSGGLEDWKAKLPDIGLPTAKGEKATLIS
                     ELKPGPYQPSTVAIGEEKDLARQRGQGFGFVRQARVEEYLSGVRAKLLAVSGKTGVPG
                     RIRIVASQDFNAYSTADGNVYVAIGCLEELESADEVAAVLAHEVSHVLLGHHSADLFA
                     DVQHRGRALYQLGIETKTMLNSRKTPSKGDGRGLDTAETVVRVTDKLVLPAWTRGQER
                     EADLLGVDLLIESGYSPAAMVSMLEKLRGWEKTDEERNKALLGRLQEPGTQTLKEKGN
                     LIYKDLLDRFATNHPKADERIGQTVTYLERHYGDRTLAEPKPGPWKALTGRPDVREVM
                     ANYRLAFSAQSALDNSKGREAYAAAKRAASGSTATDAYPNVILSRAAATVGNRPESVS
                     ALVRAVNAKEPVALLYEELIDVHERVRNFPAALGWADKASDVFAEDPQWRPHKIRLLR
                     KAGRNAEAMAATQECSLKAPEWKGLCDRANRT"
     gene            complement(3146..3412)
                     /locus_tag="VNU03_07230"
     CDS             complement(3146..3412)
                     /locus_tag="VNU03_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74289.1"
                     /translation="MPLFDCGDLSKPVLRVNPGPLKSPWAATNDKDKPTHLIAKVDGV
                     EYCVKKILVETNRVVPATKGGAECRRSGDKPPRPGFNRGVGEKC"
     gene            complement(3420..4979)
                     /locus_tag="VNU03_07235"
     CDS             complement(3420..4979)
                     /locus_tag="VNU03_07235"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HWP74290.1"
                     /translation="MINEIVDRFHPDPDLQDTAVVLFQEKNLRELGAGYPVSYAVQAE
                     VLDAIASYRPRSVFIDFVFMDERGDSQRLRDSMCNLSTVVGGRLYLAAPLDEEGLARM
                     EEKFDGCAKFATALVQSDSGVSGVLRYCSGTWGLRECAPLPKDANGTFLDSPAFAMAR
                     DRVTGIPPVAAEQMEILWPSGTTTLNRKWMTCDRSESAKARVFARLRHDPLEVKEICP
                     YTNTVSVAHLLGTFDPDVEQAIRGKAVFYGAAFGASGDRVASPTYHDLPGVYLHAMAY
                     DNLVWLDGHYLVVDRPLLQAPISWAAVVDVGVLMALVVFWVGASNVPWPGRLEGWFDP
                     EEPGYSPQGFVVRVVLTIVLLLMVVGLITRGILYSDGTVLLTVVAGYVVYKAIWKDGL
                     ALAAIVLIALAVIISYRWLHLGPRNIVGYLAFFEIARHLADHLHDGAQRYLGFRTVEY
                     DGKWGRLRWWTWLLDRLIGVWLRQDGRKRHGSLGAHPQGGRGVAAGGYRPGRAGRVGG
                     RGTVRDQEAPG"
     gene            complement(5241..5516)
                     /locus_tag="VNU03_07240"
     CDS             complement(5241..5516)
                     /locus_tag="VNU03_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74291.1"
                     /translation="MSKPTDASSDGLARALDFLAAGEWEPAHKIVQEEKSTLGSWLHG
                     IVHTLEGDLDNAQYWYRKAKREFPGPDAVQQEIAAARRTLPTGVAGD"
     gene            complement(5513..>5631)
                     /locus_tag="VNU03_07245"
     CDS             complement(5513..>5631)
                     /locus_tag="VNU03_07245"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173414.1"
                     /note="catalyzes the formation of 2-oxobutanoate from
                     L-threonine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HWP74292.1"
                     /translation="GGAAAFSALLGSAYGPAPGERVGVLVSGGNTTAVDFGR"
BASE COUNT          852 a         2045 c         1836 g          898 t
ORIGIN      
        1 ctgtaaagcc ctgcgttagc atgtggccca tgctgcctcc cggcgagggc aaccccttcc
       61 tgcgcgcgac ggtgggcgac atgctcgcga cgcaggccgc gcgcgtggga gaccgcgagg
      121 cgatcgtcgc gaccgaccgc cgcatcagct acgccgagct ctaccgggac gcccagcgtc
      181 tcgcgcgcgg gctgctcgcc tgcggcgtcc gcaaggacga caaggtggcc ctctggatgc
      241 ccggccgccc ggagtggctc gcggtccagc acggctgcgc gctgatcggc gcggtgctgg
      301 tggcgctcaa cacccgctac aaggcccacg agctgcgcta catcctgcgt cagtcggacg
      361 cgaccacgct cgtctgcgcc gatcactcgg ggccggtgga ctacctcgag accctggccg
      421 aggtgctgcc cgatctgtcc gcgtccgtcc ccggcgagct ggacgtcgcg gagttcccgg
      481 cactccggcg ggtgatcgtg cacgccgacg atccatatcc cggctgcctg gcctggcgtg
      541 acatcgagga gctcggcgat gcgcccgaga ccgccgccgc gctcgacgcc gcgcgcgcca
      601 cggtcgcgcc cgacgatcct ttcaccatcc tctacacctc cgggaccacg tccttcccga
      661 agggcgcggt catcagccac cgcaactgcc tgcctcacgg ctggtggtcc ggggcggtca
      721 tgcggctgac cgaggccgac cgcgtgctcc acgcgctccc gctctccggc acatggggcg
      781 ggctctgcat cccgctggcg accttcaccc acggagccac cctcgtcctc atggagacct
      841 tcgaggccgc ggtggcgctg cgcctgatgg aggacgaggg gatcacggtg tggaacgggg
      901 tggacgcgat ggcggtgccg atgctcgagc atcccgatct ggcgcgacgc gaccgctcgc
      961 gcctgcgcac cggcggcttc gccgcgaccg gcggcggcgt gcagggcctc ttcgaggcgg
     1021 tggtggaacg gctcggcgtg cgcctggcct tccagcccta cggcatgacc gaggtgaatg
     1081 cgatggcgct cgtccacgac ctcgacgagt cgcccgagag cctcgcggtt gcgggcgtct
     1141 ggcccgccga cggtctcgag gcgcgcgtgg tcgatccgga gaccggccgg gaccagccca
     1201 tcgacggcga gggcgagctg tggctgcgcg ggcggctggt gaccgcgggc tactaccaga
     1261 agccggagga gaccgcccgg gccttcagcg ccgacgggtg gttcaagacc ggcgacctgg
     1321 ccgtgcgcca cgcggacggc cgcgtcgtct tccgcggccg cctgcgcgag gtgctgcgca
     1381 tcagccactt catggttgcc cccggggaga tcgaggccta tctacagacc catccgaagg
     1441 tgctgcaggc gttcgtgata ggcgtgcccg acccgcgcac caacgaggcc gcggtggcct
     1501 acgtgatccc ccgccccggc tgcgcggatc tcaccgagga ggaggtgatc gcccattgcc
     1561 gcggccgcat cgcctcgttc aaggtgcccc gccacgtgcg cgtggtcgcc gacgtgccgc
     1621 gcaccccggg cccgcacggc gacaaggtgc agaaggcgcg gctgcgggag atgttcctcg
     1681 gcgggcgctg agggctacgt tcggttcgcc cggtcgcaga ggcccttcca ctccggtgcc
     1741 ttgagcgagc actcctgcgt ggccgccatc gcctcggcgt tgcggcccgc cttccggagg
     1801 aggcggatct tgtgcgggcg ccactgggga tcctccgcga acacgtccga cgccttgtcc
     1861 gcccagccga gcgccgccgg gaagttcctc acgcgctcgt ggacatcgat cagctcctcg
     1921 tagagcaggg ccaccggctc cttcgcgttg accgcgcgaa ccagggcgct gaccgactcg
     1981 gggcggttcc ccacggtggc cgccgcgcgc gacaggatca cgttcggata ggcgtcggtg
     2041 gcggtggaac cactcgcggc ccgcttcgcg gcggcgtatg cctcgcgacc cttgctattg
     2101 tcgagcgcgc tctgcgccga gaacgcgagc cggtagttcg ccatcacctc ccggacgtcg
     2161 ggccgcccgg tcagggcctt ccaggggccg ggcttcggct ccgccagggt gcggtcgcca
     2221 tagtggcgct cgaggtaggt gacggtctgg ccgatccgct cgtcggcctt cgggtgattc
     2281 gtggcgaatc gatccaggag gtccttgtag atcaggttgc ccttctcctt caaggtctgg
     2341 gtcccgggct cctggaggcg gccgagcagc gccttgttcc gctcctcgtc ggtcttctcc
     2401 catccgcgca gcttctccag catcgagacc atcgcggccg gcgagtagcc cgactcgatc
     2461 agcaggtcca ccccgagcag gtccgcctcc cgctcctgcc cacgagtcca cgcgggcaag
     2521 accagcttgt cggtgaccct caccacggtc tccgccgtgt cgagccctct tccgtcgccc
     2581 ttcgagggcg tcttccggct gttcagcatg gtcttcgtct cgatcccgag ctggtaaagc
     2641 gcccggccgc gatgctggac gtccgcgaaa agatcggcgc tgtgatggcc gagcagtacg
     2701 tgagacacct cgtgggccag gaccgccgcc acctcgtcgg cactctcgag ctcctcgagg
     2761 cagccgatcg cgacgtagac gtttccgtcc gcggtggagt acgcgttgaa gtcctggctg
     2821 gccacgatcc ggatccggcc aggcacgccg gtcttcccgg agaccgcgag cagcttcgcc
     2881 cggactccgg acaggtactc ctcgacgcgg gcctgccgca cgaatccgaa tccctggccg
     2941 cgctgccggg ccaggtcctt ctcctccccg atcgccaccg tcgagggctg atagggaccc
     3001 ggcttgagct ccgagatgag cgtggccttc tcgcccttgg cggtcggtaa cccgatatcg
     3061 ggtagcttcg ccttccagtc ctcgagcccg ccgctgcacc cgaccccgag tagcaccacc
     3121 aggccgatcc ccgcggcgtg caccgtcaac atttctcgcc cacgccccgg ttgaagccgg
     3181 gcctcggtgg cttgtcgcct gacctcctgc actccgcgcc gcccttcgtg gccggcacca
     3241 ctctattggt ctcgacgagg atcttcttca cgcagtactc cacgccgtcc accttcgcga
     3301 tcaggtgcgt cggcttgtcc ttgtcattcg tcgcggccca ggggctcttc agtgggccgg
     3361 gattcacgcg caggaccggc ttcgagagat cgccgcaatc gaagaggggc accggatcgt
     3421 caccccggag cctcctgatc tcgaactgtt cctcgtccgc cgacgcggcc ggcccggcca
     3481 ggccgataac cgccagcagc gacaccccga ccgccctgtg gatgagcgcc caatgatcca
     3541 tgccgtttcc tcccatcctg ccgtagccat actccaatca gccggtcgag caaccatgtc
     3601 caccaccgga gtctccccca cttgccgtcg tactcgacgg tccggaagcc gaggtatctc
     3661 tgcgctccgt catgcagatg gtcggcgaga tgacgggcga tctcgaagaa ggccagatat
     3721 ccgacgatgt tccgcggccc gagatgcagc cagcggtagc tgatgatcac ggcaagcgcg
     3781 atgagcacta tcgccgcgag ggccaacccg tccttccaga tcgccttgta gacgacgtag
     3841 ccggccacca cggtcagcag caccgtccca tcggagtaga ggatcccacg cgtgatcaag
     3901 ccgaccacca tgagcagcag cacgatcgtc aggaccaccc ggacgacgaa cccctgcggc
     3961 gagtacccgg gctcctccgg gtcgaaccag ccctccaggc gccccggcca cggcacgttg
     4021 ctcgcgccta cccagaacac gacgagcgcc atcagcaccc ccacatcgac caccgcggcc
     4081 cacgagatcg gtgcttgcaa aaggggccgg tccacgacca ggtagtgacc gtcgagccac
     4141 acgagattgt cgtacgccat cgcgtggagg tagactccgg gcagatcgtg atacgtcggc
     4201 gatgccacgc gatcccccga ggcgccgaac gcagctccgt agaagacggc cttgcctcgg
     4261 atcgcctgct ccacgtccgg atcgaaggtc ccgaggagat gcgccaccga aaccgtgttc
     4321 gtgtaggggc agatctcctt cacctccagt gggtcgtgcc ggagccgggc gaacacgcgg
     4381 gccttcgcac tctccgaccg gtcacacgtc atccacttcc gattcaaggt ggtggtcccg
     4441 ctcggccaca ggatctccat ctgctccgcg gccaccggag ggatcccggt gactcgatcc
     4501 ctcgccatcg cgaaagccgg gctgtccagg aatgtcccgt tcgcgtcctt cggcagcggt
     4561 gcgcactccc ggaggcccca cgttccgctg caatacctca gtactccgct gaccccggaa
     4621 tccgactgca cgagcgcggt cgcgaacttc gcgcacccat cgaacttctc ctccatcctg
     4681 gcgagcccct cctcatccag gggcgcggcc aggtagaggc gtccgcccac caccgtggac
     4741 aggttgcaca tggagtcccg caagcgctgc gagtcccctc gttcatccat gaacacgaag
     4801 tcgatgaaaa ccgatctcgg gcggtaggac gcaatggcgt cgagaacctc cgcctgcacg
     4861 gcgtatgaca ccgggtaacc cgcccccagc tcacggaggt tcttctcctg gaacaggacc
     4921 accgccgtat cctggaggtc ggggtccggg tggaaccgat cgacgatctc gttgatcatc
     4981 acctgcgacc agggcgtcaa gtcgaacgag atgccgtgga ggttcaggac cgcgaggccg
     5041 accagcagga acaacatgga ttgcaccgcg atccgcactt cccagcgggg gatgcgcggg
     5101 gaccggcggg agtcgcgtcc ggaggacgag tatcggccct cggatggcat cgagtcggga
     5161 cagtctagtg cgattctccg ggccgtcaag ggggaagcca ccgtgtaagg acaccgccgg
     5221 gagccggcgg ggataccgcg ctagtcgccc gctactccgg tcggcagcgt ccggcgcgcg
     5281 gccgcgatct cttgctggac cgcgtccggg ccggggaact cgcgcttggc cttgcgatac
     5341 cagtactgcg cgttgtcgag gtctccctcg agcgtgtgca cgataccgtg cagccaggag
     5401 ccgagcgtgg acttctcctc ttggacgatc ttgtgcgccg gctcccactc gccggcggcc
     5461 aggaagtcga gcgcccgggc gagcccgtca gacgaggcgt cggtaggctt gctcatcggc
     5521 cgaagtctac cgcagtcgtg ttgcctccgc tcaccaggac gccgacccgc tcgcccgggg
     5581 ccggcccgta ggctgagccg agcagcgcgg agaaggccgc ggcaccaccg g
//
LOCUS       DATMCV010000310         2739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_151374, whole genome shotgun sequence.
ACCESSION   DATMCV010000310 DATMCV010000000
VERSION     DATMCV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151374"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VNU03_07250"
     CDS             <1..248
                     /locus_tag="VNU03_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HWP74293.1"
                     /translation="LIENMINPAVAGHGGFVQLVDVKDNKVYLQMGGGCQGCGAADVT
                     LKQGIERLIKEELPEIEEVLDTTDHDSGENPYYTPSK"
     gene            complement(263..889)
                     /locus_tag="VNU03_07255"
     CDS             complement(263..889)
                     /locus_tag="VNU03_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HWP74294.1"
                     /translation="MTRRGRVALKVVAWAACLAPLGWLGYRAGTGDLTANPISFITNW
                     LGDWTLRILLVSLAMTPLRTLSGWSWPIALRRLLGLFALAYASLHFGVWLILDHFFAW
                     KQMGADIVKRPYITVGMTALVLMIPLAATSTAGAIRRLGGARWRWLHRLVYVTGVCAV
                     LHYLWLAKKANPAPYYYAAAFIVLMAIRAWDWTRRASKNPLPGGERVG"
     gene            complement(891..1856)
                     /gene="msrP"
                     /locus_tag="VNU03_07260"
     CDS             complement(891..1856)
                     /gene="msrP"
                     /locus_tag="VNU03_07260"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HWP74295.1"
                     /translation="MLIKRGGDIRGGEITDEALYLNRRAFMAGAAAVALASGVAAPGM
                     AAAAPPAAGQPLAATRNKAFDIPDAATKFQDATTYNNFYEFGVNKDDPSSLAGTLKPR
                     PWKVQVDGLVHKPATFDVDDVLKMAPLEERVYRLRCVEGWSMVIPWIGFPLSVLLKRV
                     DPTGSAKYVEFSTLVDPNQFPGQKKGLFNFGGLDWPYTEGLRLDEALHPLTLLTVGMY
                     GQVLPNQNGAPIRVVIPWKYGFKSAKSIVRIRVTAEEPKTAWNKAAPQEYGFYSNVNP
                     KVDHPRWSQATERRIGEFRRRETLMWNGYDQVAGLYTGMDLKKQY"
     gene            2032..2721
                     /locus_tag="VNU03_07265"
     CDS             2032..2721
                     /locus_tag="VNU03_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWP74296.1"
                     /translation="MKIRGCVTALLAALLLAACATAPEGTYFATPSDPSTPRMAQILS
                     RAAVAGGDDPARYGFAFIKSPTAAAYSDEDAVFYFTDGLMRQPTPVIEAVVAHEVAHE
                     VLGHVGTRRKLSLSLTASFGALGLFAPGAGLLDFVVNPLVVRAFGRYQEMQADQKAVE
                     ILRAMGYRSPRRALASALESLAAYAPKEREGLEGLLATHPSLDERLAALEPLEGPAKP
                     VNPVARVRPPR"
BASE COUNT          418 a          973 c          933 g          415 t
ORIGIN      
        1 acctgatcga gaacatgatc aatccggcgg tggccggcca cggcggattc gtgcagctgg
       61 tcgacgtcaa ggacaacaag gtctacttgc agatgggtgg cggctgtcag ggctgcggag
      121 ccgcggacgt cacgctcaag cagggcatcg agcggctgat caaggaagag cttcccgaga
      181 tcgaggaggt gctggacacc accgatcacg actcgggcga gaacccgtac tacacgcctt
      241 ccaagtaggc ccgcctcgcc cctcacccca ccctctcccc tccggggaga gggtttttgc
      301 ttgcccggcg cgtccagtcc caggcgcgaa tcgccatcag cacgatgaac gccgccgcgt
      361 agtagtaggg cgccgggttc gccttcttgg cgagccagag atagtgcagc accgcgcaca
      421 ccccggtcac gtagaccagc cggtgcagcc accgccagcg cgcgccgccg agccggcgaa
      481 tggcccccgc ggtggaggtg gcggccaggg ggatcatcag caccagcgcg gtcatgccca
      541 ccgtgatgta gggccgcttc acgatgtcgg cgcccatctg cttccaggcg aagaaatggt
      601 cgaggatgag ccacacgccg aagtgcagcg aggcgtaggc gagcgcgaag aggcccagca
      661 gccggcgcag cgcgatcggc cacgaccagc cggacagcgt gcgcagcggg gtcatggcca
      721 ggctgacgag caggatgcgc agcgtccagt cgcccagcca gttggtgatg aagctgatcg
      781 ggttggcggt caggtcgccg gtgccggcgc ggtagccgag ccatccgagc ggggccaggc
      841 acgcggccca cgcgaccacc ttcagcgcga cgcggccgcg tcgggtcacc ctagtactgc
      901 ttcttcagat ccatccccgt gtagagcccc gccacctggt cgtagccgtt ccacatgagc
      961 gtctcgcgac ggcggaactc gccgatgcgc cgctcggtgg cctggctcca gcgcgggtgg
     1021 tccaccttcg ggttgacgtt ggagtagaag ccgtactcct ggggcgccgc cttgttccag
     1081 gccgtcttcg gctcctcggc ggtgacccgg atgcgcacga tggacttggc gctcttgaag
     1141 ccgtacttcc acggaatcac gacccggatc ggcgcgccgt tctggttcgg cagcacctga
     1201 ccgtacatcc caaccgtgag cagggtcagc gggtggagcg cctcgtcgag acgcaagccc
     1261 tcggtgtagg gccagtcgag gcccccgaag ttgaagaggc ccttcttctg gccgggaaac
     1321 tgattgggat ccacgagggt cgagaactcg acgtacttgg ccgagccggt cgggtccacg
     1381 cgcttgagga gcacggagag ggggaagccg atccacggga tgaccatgga ccacccctcg
     1441 acgcagcgca gccgatagac gcgctcctcg agcggagcca tcttcagcac gtcgtcgacg
     1501 tcgaaggtcg cgggcttgtg cacgaggccg tccacctgca ccttccacgg ccggggcttg
     1561 agcgtcccgg ccaggctcga cgggtcatcc ttgttgacgc cgaactcgta gaagttgttg
     1621 taggtggtgg cgtcctggaa cttggtcgcc gcgtccggga tgtcgaaggc cttgttgcga
     1681 gtggccgcga gcggctggcc ggcggccggt ggcgcggcgg cggccatccc cggcgcggcc
     1741 acgcccgagg ccagcgccac cgcggcggct cccgccatga aggcgcgccg gttcaggtac
     1801 agcgcctcgt cggtgatctc cccgcccctg atgtccccgc cccgcttgat gagcatgtcg
     1861 acccgtcccc ctgtgtcgcc gagtgtagca gccgcccgga ccgggatgtc acgacggtgt
     1921 gaaccctcgc cccccaccgg gagagggcgt ccgcgtggcc gccccaagcc ctagaagccc
     1981 ttatttcctc gggccctgcc ggggccgcct tctaagatga aggcggccat catgaagatt
     2041 cgcggctgcg tcaccgcgct gctggctgcc ctgctgctgg cggcctgcgc caccgcgccg
     2101 gaggggacct atttcgccac gccctccgac cccagcacgc cgcggatggc ccagatcctg
     2161 agccgcgccg ccgtcgcggg cggcgacgat ccggcccgct acggcttcgc gttcatcaag
     2221 agcccgaccg ccgccgccta cagcgacgag gacgcggtct tctacttcac cgacgggctc
     2281 atgcgccagc ccaccccggt catcgaggcg gtggtcgcgc acgaggtcgc ccacgaggtg
     2341 ctgggccacg tgggcacccg ccgcaagctc tcgctctcgc tcaccgcgag cttcggcgcg
     2401 ctcggcctct tcgcgcccgg cgccgggctg ctcgacttcg tggtgaaccc gctggtggtc
     2461 cgcgccttcg ggcgctatca ggagatgcag gccgatcaga aggccgtcga gatcctgcgc
     2521 gcgatgggct accgctcgcc gcgccgcgcc ctcgcctccg cgctcgagtc gctggccgcc
     2581 tacgcgccca aggagcgaga ggggctcgag gggctgctcg ccacgcatcc ctcgctcgac
     2641 gagcggctcg ccgcgctcga gccgctcgaa gggccggcga agccggtcaa tcccgtcgcg
     2701 cgggtgcgcc cgccgcgata ggttgcccct cgatccctc
//
LOCUS       DATMCV010000311         5308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_151381, whole genome shotgun sequence.
ACCESSION   DATMCV010000311 DATMCV010000000
VERSION     DATMCV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151381"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VNU03_07270"
     CDS             complement(<1..454)
                     /locus_tag="VNU03_07270"
                     /inference="COORDINATES: protein motif:HMM:NF015412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP74297.1"
                     /translation="MLKVGIVGMGVIGRHVADALTRGIPGTTLVGVSVRQPATATGYP
                     MLALDELIERADLIVEAATQAALREFGPAVLSAGKHLMVLSVGALVGVLDGWAALAEK
                     HGCRILVPSGAIAGLDGVKGAREGAITAVTMETRKPPRGLAGAPYIEQQ"
     gene            547..1431
                     /locus_tag="VNU03_07275"
     CDS             547..1431
                     /locus_tag="VNU03_07275"
                     /inference="COORDINATES: protein motif:HMM:NF021210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HWP74298.1"
                     /translation="MTATPRLQEPLERLYREFDWAARADLDAIRFPLRYPERADREVV
                     ALLASCMAYGRVDLFGPWVDWALARMGESPARFVLGFDPAKHAARFEDFRYRFNRPRD
                     LAAFCLATQRVLIRHGSLGAAFCSGFSPGDAHVGPALERFVDAFLTQDLSAVFPRNRL
                     SYGYRHWFPRPSTGGACKRMLLFLRWMVRREAPDFGLWTEIPTAALLIPVDTHIENMA
                     RSLGLTRRRSRNWKMTEEITSGLRLLDPADPVKYDFSLCHTRMSGQCLDRRDAIVCAP
                     CRLRPVCRHWRGRRSAAR"
     gene            1428..2531
                     /locus_tag="VNU03_07280"
     CDS             1428..2531
                     /locus_tag="VNU03_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWP74299.1"
                     /translation="MTAGWVWVVVAALALLLGVTAWALWRAVARLGALETARQQPDQA
                     LLLLQRDIQTARAEAHQARSETLATVKEELHQFASHMTQQMGQVGSGVQQQLQHVGQV
                     VGAVQGSLGKLGEVTQRVFDVGRDIAGLEQLLKSPKIRGGVGETLLENLLAQMLPREQ
                     YSLQHAFATGDRVDAAIRIGERIVPVDAKFPLENFRRVLEETDEDKRGPLRRAFLRDV
                     KNRVDEIAKKYILPDEGTFDFALMYIPAENIYYEIIVRDDSEEDSPAAYALARRVVPV
                     SPNTFYAYLRVIVLGLRGLRIERDAQEIQARLGRLRGDLDKFREAFDVVGRHLTNARN
                     KYDDAASGLGRLEGKLEGIERHGEQGALPGIAP"
     gene            2620..2922
                     /locus_tag="VNU03_07285"
     CDS             2620..2922
                     /locus_tag="VNU03_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74300.1"
                     /translation="MPDIQFQVYGINLLVRLMAEPPADVRVHCPKGSPIRYGEVVGRG
                     DGFDEGANAFRDMPPVRSVVAFEENPEEVEGHYFYISGEEHRVIRLDAIILSFPLE"
     gene            2935..4167
                     /locus_tag="VNU03_07290"
     CDS             2935..4167
                     /locus_tag="VNU03_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWP74301.1"
                     /translation="MTALSQVLRKVDADEVVELTRALVRIPSVYRPGDPEATEARVAD
                     FVESWLRREGFQVEIQPVAPGRPNVIGSLGEKRPGQKSLLLEGHTDVVTEGDPAEWSH
                     PPFGADLVDGRIYGRGTADMKSGLAAAMVAAAAFKRAGVGLRGKLVVGALVDEEDGMI
                     GVRHLCETAVGRELDAAIICEPEENELCLEQRGVVWARFRVRGKMAHGAMPEAGINPI
                     ACLGRLLAAAPGLERRLRKACEKSRYLRPPTVTPTIIQGPPPRVGVPQSNVIPAVAET
                     TFDIRLTPGIGDDGIRAELEAICRDAAAAQPGIKVEWEPVNAFRLATKVDRSEALVEA
                     MARGVKQATRRAPRYGGVPGSTDGTILRMQLGIPIVTCGPGDRLIPHQVDEFVAADQV
                     VEAARIYVASALNYLEAE"
     gene            4164..>5308
                     /gene="amrB"
                     /locus_tag="VNU03_07295"
     CDS             4164..>5308
                     /gene="amrB"
                     /locus_tag="VNU03_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HWP74302.1"
                     /translation="MSPAGNPRLRAVEAFPVEHEGQRCIALRDPAGFTDQIAVLPGPL
                     LDLVSLFDGEHPVQAIRDILRRRHGEAPSVEQIAALVDRLDDAGFLDSERFRARRRAT
                     DDAFRQSPVRPAAHAGGAYAGEAAALREQIAGFFAADRSDKRAGTLSALIAPHIDFHR
                     GGTTYGSAYQEVLDRSDADLYVILGTCHAGMSDPFAVTLKPYDTPLGPVGVDRDFYEA
                     LAGRAGQDLLASEPAHRAEHSIEFQAVMLQSLLGGRRPFTILPVLASYLHEAVWAGTE
                     PEADPRVPRFLDALREAVVASSRRVCLVAGVDLAHVGPRFGDPDPNTEASLAIVERED
                     RAMLESVVAGDARGFFAGVAADRDARRICGLSPIYSLLRVLPEAPGRL"
BASE COUNT          708 a         1944 c         1877 g          779 t
ORIGIN      
        1 gctgctgctc gatgtagggc gcccccgcga gcccgcgcgg cggcttgcgg gtctccatgg
       61 tgaccgcggt gatcgcgcct tcccgtgcgc ccttgacgcc gtcgaggccg gcgatcgcgc
      121 cggatggcac caggatgcga cacccgtgct tctcggccag cgcggcccac ccgtcgagca
      181 cgccgaccag ggcgcccacc gagaggacca tcaggtgctt gccagccgag agcaccgcgg
      241 gcccgaactc gcggagcgcg gcttgggtcg ccgcctccac gatcaggtcg gcccgctcga
      301 tcagctcgtc gagggccagc atggggtagc cggtcgcggt ggcgggctgg cgcacgctga
      361 cgcccaccag cgtggtgccg ggaatgcccc gcgtcagtgc gtccgcgacg tggcggccga
      421 tcacgcccat ccccacgatt cccaccttca gcatgcgctc gtccttcccg ccgcgtgcgc
      481 ggcgcagcga tgatagcagc gggcgccggg ccgggacaag ggaccgtgct agaatcagcg
      541 ccgaagatga ctgcaacgcc ccggctccag gagccgctcg agcggctcta ccgcgagttc
      601 gactgggccg cccgcgccga cctggacgcg atccgctttc cccttcgcta ccccgagcgc
      661 gccgatcgcg aggtcgtcgc gctgctggcc tcctgcatgg cctacgggcg cgtggatctg
      721 ttcggcccct gggtcgactg ggcgctcgcc cgcatgggag agtcgcccgc gcgcttcgtg
      781 ctgggcttcg atccggccaa gcacgcggcg cgcttcgagg acttccgcta ccggttcaac
      841 cgtccgcgcg atctcgccgc gttctgcctc gccacccagc gggtcctgat ccggcacggc
      901 tcgctcggcg ccgcgttttg ctccggtttc tcgcccggcg acgcccacgt gggcccggcc
      961 ctggagcgct tcgtcgacgc tttcctgacc caggacctct ccgcggtctt tccgcgcaac
     1021 cggctctcgt acgggtaccg tcactggttc ccgcgcccgt ccaccggcgg cgcgtgcaag
     1081 cggatgctgc tcttcctccg gtggatggtc cgccgggagg cccccgactt cgggctctgg
     1141 accgagatcc cgaccgccgc gctcctgatc ccggtggaca cccacatcga gaacatggcc
     1201 cggtcgctcg gtctcacccg gcggcgtagc cggaactgga agatgacgga ggagatcacg
     1261 agcgggctgc gcctgctcga cccggccgat ccggtgaagt acgacttctc gctctgccac
     1321 acccggatgt ccggccagtg cctcgaccgc cgcgacgcga tcgtgtgcgc cccgtgccgg
     1381 ctgcggccgg tgtgccgcca ctggcgcggg cgacggagcg cggcccggtg accgcggggt
     1441 gggtgtgggt cgtggtggcg gccctcgcgc tgctgctcgg ggtgaccgcg tgggccctct
     1501 ggcgcgcggt ggcgcggctc ggcgcgctcg agacggcccg gcagcagccg gatcaggcgc
     1561 tgctcctgct ccagcgcgac atccagaccg cgcgggcgga ggctcaccag gcgcggagcg
     1621 agacgctcgc caccgtgaag gaggagctgc accagttcgc gagccacatg acccagcaga
     1681 tgggccaggt cggcagcggc gtccagcagc agctccagca cgtgggccag gtggtgggcg
     1741 cggtgcaggg gagcctcggg aagctgggcg aggtgaccca gcgcgtcttc gacgtggggc
     1801 gggatatcgc gggcctcgag cagctgctca agtcgcccaa gatccggggc ggggtggggg
     1861 agacgctcct cgagaacctc ctcgcccaga tgctcccgcg ggagcagtac tcgctgcagc
     1921 acgcgttcgc caccggggac cgggtcgacg cggccatccg catcggggag cggatcgtcc
     1981 cggtcgacgc caagttcccc ctggagaact tccggcgcgt cctcgaggag accgacgagg
     2041 acaagcgcgg cccgctgcgg cgcgcgttcc tgcgcgacgt caagaaccgg gtcgacgaga
     2101 tcgccaagaa gtatatcctg ccggacgagg gcaccttcga cttcgccctc atgtacatcc
     2161 cggccgagaa catctactac gagatcatcg tccgggacga cagcgaggag gattcgcccg
     2221 ccgcgtacgc gctggcccgc cgggtggtgc cggtctctcc caacacgttc tacgcgtacc
     2281 tgcgggtcat cgtcctgggg ctgcgcgggc tgcggatcga gcgcgatgcc caggagatcc
     2341 aggcgcggct cggccgcctg cgcggggatc tcgacaagtt ccgcgaggcg ttcgacgtgg
     2401 tgggccggca tctcaccaat gcccgcaaca agtacgacga tgccgcctcc ggcctgggcc
     2461 ggctcgaggg caagctggag ggcatcgagc gccacggcga gcagggcgcc ctgcccggca
     2521 tcgcgccctg accgccgagc tccacctcgc gggccctccg ccgaattcgg gaaccagcgg
     2581 ttgtggtatg atcgaagaaa tccaccatca gagggtccca tgcccgacat tcagttccag
     2641 gtctacggca tcaacctcct cgtccgactc atggccgagc ccccggcgga cgtgcgtgtg
     2701 cactgcccca aggggtcgcc gatccgttac ggcgaggtgg tgggccgtgg cgacggcttc
     2761 gacgaggggg ccaatgcctt ccgcgacatg ccgccggtcc gctcggtggt cgccttcgag
     2821 gagaaccccg aggaagtgga agggcactac ttctacatct cgggtgaaga gcatcgcgtc
     2881 atccgcctcg acgccatcat cctctcgttc ccgctggaat agcccgctcc cgctgtgacc
     2941 gcgctgtcgc aggtcctccg caaggtcgac gcggacgagg tggtcgagct cacccgcgcc
     3001 ctcgtccgga tccccagcgt gtatcgtccc ggcgaccccg aggcgaccga ggcgcgggtc
     3061 gccgacttcg tggagtcgtg gctccgccgc gagggcttcc aggtcgagat ccagccggtc
     3121 gctcccggcc gtccgaacgt gatcggctct ctcggcgaga agcggcccgg ccagaagagc
     3181 ctgctcctcg aagggcacac cgatgtggtg accgaggggg atccggcgga gtggtcgcat
     3241 cccccgttcg gcgccgacct ggtcgacggg cggatctacg ggcgcggcac cgcggacatg
     3301 aagagcggcc tggccgccgc gatggtggcc gccgccgcgt tcaagcgggc cggcgtcggc
     3361 ctccggggca agctggtggt gggcgcgctc gtcgacgagg aggacggcat gatcggcgtg
     3421 cgccacctct gcgagaccgc ggtcggccgc gagctggacg ccgccatcat ctgtgaaccc
     3481 gaggagaacg agctctgcct ggagcagcgc ggggtggtct gggcgcggtt ccgggtgcgc
     3541 ggcaagatgg cccacggggc catgcccgag gcgggcatca atcccatcgc gtgcctcggc
     3601 cggctgctgg ccgccgcccc cggcctcgag cggcggctgc ggaaggcctg cgaaaagagc
     3661 cgctatctgc ggccgcccac cgtgacgccg accatcatcc agggcccgcc gccccgggtc
     3721 ggagtcccgc agtccaacgt gatccccgcg gtggccgaga cgacctttga catccgcctc
     3781 acgccgggca tcggcgacga cggcatccgc gccgagctgg aggcgatctg ccgcgacgcc
     3841 gcggccgccc agccggggat caaggtggag tgggagccgg tcaacgcgtt ccgcctcgcc
     3901 accaaggtcg atcgctccga ggccctcgtg gaggccatgg cccgcggcgt gaagcaggcc
     3961 acgcgccggg ccccgcggta cggcggcgtg cccggctcca ccgacggcac gatcctccgg
     4021 atgcagctcg gcatcccgat cgtcacctgc gggcccggtg accgcctgat cccgcaccag
     4081 gtcgacgagt tcgtcgccgc ggatcaggtc gtcgaggcgg cccggatcta cgtcgcgtcc
     4141 gcgctcaact acctcgaagc cgagtgagcc ccgccggcaa tccgcgcctg cgggctgtcg
     4201 aggcctttcc ggtcgagcac gaagggcagc gctgcatcgc gctgcgggat cccgccggct
     4261 tcaccgacca gatcgcggtg ctgccggggc ccctgctcga cctggtctcc ctcttcgacg
     4321 gtgagcaccc ggtgcaggcg atccgcgaca tcctgcgccg gcgtcacggc gaggcgccct
     4381 cggtcgagca gatcgccgcg ttggtggacc ggctcgacga cgcgggcttc ctcgacagcg
     4441 agcggttccg ggcgcggcgc cgcgcgaccg acgacgcctt ccggcagagc ccggtgcggc
     4501 ctgcggccca cgcgggcggt gcgtacgcgg gcgaggcggc ggcgctgcgc gagcagatcg
     4561 cgggcttctt cgccgccgac cggtccgaca agcgcgcggg gaccctctcg gccctgatcg
     4621 ctcctcacat cgattttcac cgtgggggaa caacttacgg ctccgcgtat caggaagtgc
     4681 tcgatcgatc ggatgccgat ctctacgtca ttctcgggac gtgccacgcc gggatgtccg
     4741 acccgttcgc ggtcaccctc aagccgtacg acaccccgct cggccccgtc ggcgtcgacc
     4801 gggacttcta cgaggccctt gccgggcggg ccggccagga tctgctcgcc tccgagccgg
     4861 cgcatcgcgc cgagcattcc atcgagttcc aggcggtcat gctccagtcc ctgctgggcg
     4921 gccgccggcc attcaccatc ctcccggtgc tcgcctcgta cctgcacgag gcggtgtggg
     4981 ccggcaccga gccggaggcc gacccgcgcg tgccccgctt cctcgacgct ctccgggagg
     5041 cggtggtggc ctcgtcccgt cgcgtctgcc tggtggcggg cgtggatctg gcccacgtcg
     5101 gcccgcgctt cggcgatccc gacccgaaca cggaggcgtc cctggcgatc gtcgagcgcg
     5161 aggaccgggc gatgctcgag tcggtggtgg ccggcgacgc ccgcggcttc ttcgccgggg
     5221 tggcggccga ccgcgacgcg cggcgcatct gcggcctctc gccgatctac tcgctgctcc
     5281 gggtcctgcc ggaggcgccg gggcgtct
//
LOCUS       DATMCV010000312         3544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_482936, whole genome shotgun sequence.
ACCESSION   DATMCV010000312 DATMCV010000000
VERSION     DATMCV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482936"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1020
                     /locus_tag="VNU03_07300"
     CDS             115..1020
                     /locus_tag="VNU03_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HWP74303.1"
                     /translation="MAKWRVHPDEEPSRQAQALAEQVERDGGTVLTIYQEPVGDHWQI
                     FALLPMDKVEPTPYQRDLSPTHAKKLQEVVKKIDRFVDPVVVMSPRPGVYWTPNGNHR
                     RVVLGKLKAKVIPAILVPEAEVAFQILALNTEKAHNLKEKSLEVIRMYRGLVDEAPSE
                     GEEGYAFQFESAHFITLGLLYEQNKRFAGGAFAPILRRVDKFLKGHFPKTLKEREERA
                     ALVREADEALVGAVARIKKRGISHPYVKNFVLARTTPLTRQRKTLPGFEQTFRKLVDN
                     IEAFDVSKVRYEDIQRSAIMAVPAG"
     gene            1025..1336
                     /locus_tag="VNU03_07305"
     CDS             1025..1336
                     /locus_tag="VNU03_07305"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWP74304.1"
                     /translation="MARPSKLAAAEITSHLSTLPGWSIAGGKLHRELTFADFAQAFAF
                     MTEVAREAEALQHHPEWFNVYNRVVIDLNTHDAGGITTLDFELARRVEDLAARRARPA
                     P"
     gene            1333..2028
                     /locus_tag="VNU03_07310"
     CDS             1333..2028
                     /locus_tag="VNU03_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP74305.1"
                     /translation="MTGRVAIVTGGTGALGQAITGALLEAGATVAIPYAVAEERAALE
                     GRLAPELRARLLPVAADVTDEAAVAKFVRTVHERHSRVDGLVNGVGGFAGGDLVSTPL
                     AEWERMMKLNLTSTVIACRAVLPGMIAGGGGRIVNIASRAVLPPQGGFIAYTVSKAAV
                     ITLTQALAQEVKPHRITVNAVLPSTMDTPANRRAMPDADRSGWVSTADVASVVAFLLS
                     DRAAAVTGASLTV"
     gene            2043..3440
                     /locus_tag="VNU03_07315"
     CDS             2043..3440
                     /locus_tag="VNU03_07315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP74306.1"
                     /translation="MERFDLVVIGAGAAGITAAKTAAELGARVALVDRGPLGGLCINR
                     GCIPKKALVTAGRVHRMVREAASFGTAPGSGQLDWDAVQRRQREVVETLRPAAGDLEK
                     RGIRVAVGTGRFSDPHIVEVDGTPLGAERFVIAAGSEPVIPEVPGRERLITSDQLLFL
                     PSFPASLTFIGGGAVAMELAGAYTDLGSRVTVLARDAEILPGVDPDLARTLRKRMEAR
                     GVTFRLQTTVTRLAADPAGVRLEFDSAGMANRMTSSQVCAAVGRRFHPRSVGAARIGL
                     EMGRLGLRTTPYLATSVPHIYAAGDAAGNRQLTPVAAHEGRIAAINALRGDVERADEA
                     VIPQVLFTTPEVGLVGLSYAEAPAHGIQAAVGRHDARGASNSVATGDEADYFKLVFDQ
                     DTQRLIGAQIVSSAAAELIQLCALAVRSRVPAGLVAGQLSVHPSHGERLLRAFGPGHG
                     MAHPPEGVGVVESRP"
BASE COUNT          498 a         1313 c         1255 g          478 t
ORIGIN      
        1 cccctccccg gtagttatgc cggaattgat aatcggccag accaattcat tgcggaatct
       61 tcgtaagtac gcgtcacgtt gacaggttcc cggagcggat tcatactccc tcgcatggcg
      121 aagtggcggg tccatcccga cgaggagccg agccggcagg cgcaggcgct ggcggagcag
      181 gtcgagcgcg acggcggcac ggtgctgacg atctaccagg agccggtcgg cgatcactgg
      241 cagatcttcg cgctgctccc gatggacaag gtggagccga ccccgtacca gcgcgatctc
      301 tccccgaccc acgccaagaa gctgcaggaa gtggtcaaga agatcgatcg cttcgtcgat
      361 ccggtggtgg tcatgtcgcc ccgccccggc gtgtactgga ctcccaacgg caaccaccgg
      421 cgggtggtcc tcggcaagct caaggccaag gtcatccccg cgatcctggt gcccgaggcc
      481 gaggtggcct tccagatcct cgcgctcaac accgagaagg cgcacaacct caaggagaag
      541 tccctcgagg tcatccgcat gtaccgcggg ctcgtggacg aggcgccgag cgagggcgag
      601 gagggctacg ccttccagtt cgagtccgcc cacttcatca ccctgggcct gctctacgag
      661 cagaacaagc gcttcgcggg cggcgcgttc gcgccgatcc tgcgccgggt ggacaagttc
      721 ctgaagggcc acttcccgaa gacgctcaag gagcgggagg agcgggccgc gctcgtgcgg
      781 gaggccgacg aggcgctggt gggggcggtg gcccggatca agaagcgcgg gatcagccac
      841 ccgtacgtca agaacttcgt cctcgcgcgc accacaccgc tcacccggca gcgcaagacg
      901 ctccccggct tcgagcagac gtttcgcaag ctcgtcgaca acatcgaggc cttcgacgtc
      961 tccaaggtgc gctacgagga catccagcgc tcggccatca tggccgtgcc ggcgggctga
     1021 gccgatggcc cgcccgagca agctcgccgc ggccgagatc acgtcgcacc tgtccacgct
     1081 gcccggctgg tcgatcgcgg gcggcaagct ccaccgcgag ctcaccttcg cggacttcgc
     1141 ccaggccttc gcgttcatga ccgaggtggc ccgcgaggcc gaggccctcc agcaccatcc
     1201 ggagtggttc aacgtctaca accgcgtggt gatcgatctc aacacccacg acgcgggcgg
     1261 catcaccacc ctcgacttcg agctggcccg gcgggttgag gatctggccg cccgccgcgc
     1321 ccggcccgcg ccatgaccgg tcgggtcgcg atcgtcaccg gcggcaccgg cgcgctcggc
     1381 caggcgatca ccggggccct gctcgaggcc ggcgccaccg tcgcgattcc ctacgcggtg
     1441 gccgaggaac gcgccgcgct cgaggggcgg ctcgcgcccg agctgcgcgc gcggctcctg
     1501 ccggtggccg ccgacgtgac cgacgaggcg gcggtggcca agttcgtgcg gacggtccac
     1561 gagcggcaca gccgggtcga cggcctggtc aacggggtgg gcggcttcgc gggcggcgac
     1621 ctcgtctcga cgccgctcgc cgagtgggag cggatgatga agctcaacct cacctccacc
     1681 gtgatcgcct gccgcgcggt gctgcccggc atgatcgcgg ggggcggcgg gcgcatcgtc
     1741 aacatcgcct cgcgcgcggt gctgccgccc cagggcggct tcatcgccta caccgtctcc
     1801 aaggccgcgg tcatcaccct cacccaggcc ctggcccagg aagtgaagcc gcaccggatc
     1861 accgtgaacg cggtgctgcc gagcaccatg gacacgccgg ccaaccgccg cgcgatgccc
     1921 gacgccgacc gctccggctg ggtcagcacc gccgacgtgg cgagcgtggt ggccttcctg
     1981 ctgtccgacc gcgcagccgc cgtcaccggc gcgagcctca ccgtctgagc ggggcccgct
     2041 cgatggagcg cttcgacctc gtggtcatcg gggccggggc cgccgggatc acggcggcca
     2101 agaccgccgc cgagctgggc gcacgcgtcg ccctcgtgga ccgcggcccg ctgggcggtc
     2161 tctgcatcaa ccgcggctgc attcccaaga aggcgctcgt caccgccggc cgcgtgcacc
     2221 gcatggtccg cgaggccgcc tcgttcggca ccgcgccggg gagcgggcag ctcgactggg
     2281 atgcggtgca gcggcgccag cgggaggtgg tggagaccct ccggcccgcc gccggcgacc
     2341 tcgagaagcg cggcatccgg gtggcggtcg gcaccgggcg gttcagcgat ccccacatcg
     2401 tggaggtgga cggcaccccg ctcggcgcgg agcgcttcgt gatcgcggcc ggctcggagc
     2461 cggtgatccc cgaggtgccg ggccgcgagc ggctcatcac ctcggaccag ctcctcttcc
     2521 tcccctcgtt ccccgcctcg ctcaccttca tcggcggcgg ggcggtggcg atggagctgg
     2581 cgggcgccta caccgacctg ggctcgcgcg tcacggtgct ggcccgcgac gcggagatcc
     2641 tgccgggggt ggaccccgac ctggcgcgca cgctgcgcaa gcggatggag gcccgcgggg
     2701 tcacgttccg cctgcagacc accgtgaccc ggctcgcggc ggatccggcc ggcgttcgcc
     2761 tggagttcga cagcgcgggc atggcgaacc ggatgacgag cagccaggtg tgcgcggcgg
     2821 tgggccgtcg cttccaccca cgcagcgtcg gcgccgcgcg catcgggctc gagatggggc
     2881 ggctcgggct gcgcacgacc ccgtacctgg ccacctcggt gccccacatc tacgcggcgg
     2941 gcgacgcggc cggcaatcgc cagctcaccc cggtggccgc ccacgagggc cggatcgccg
     3001 cgatcaacgc cctgcggggc gacgtggagc gggccgacga ggcggtgatc ccgcaggtcc
     3061 tcttcacgac gcccgaggtg ggcctggtcg ggctctcgta cgcggaggcc cccgcgcacg
     3121 gcatccaggc cgcggtgggc cgtcacgacg cgcgcggggc cagcaacagc gtcgccaccg
     3181 gtgacgaggc ggactacttc aaactggtct tcgaccagga cacccagcgg ctcatcggcg
     3241 cgcagatcgt ctcctccgcc gccgcggagc tgatccagct ctgcgcgctg gcggtgcgct
     3301 cgcgcgttcc cgccggcctg gtggcgggcc agctctcggt gcacccgagc cacggggagc
     3361 gcctgctgcg ggccttcggg cccgggcacg gcatggcgca cccccccgag ggcgtgggcg
     3421 tagtagaatc gcgcccgtga ctccactgcc gctccggcgc gcggccctct ggctgatggc
     3481 cgccgtcctc ctcgccggct gcgcgggctc gggcgcccgc gccgaccggt ccgcctccgc
     3541 ggac
//
LOCUS       DATMCV010000313         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_617153, whole genome shotgun sequence.
ACCESSION   DATMCV010000313 DATMCV010000000
VERSION     DATMCV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617153"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VNU03_07320"
     CDS             <1..174
                     /locus_tag="VNU03_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWP74307.1"
                     /translation="IQAGSGAVFSLLPPENATGNFVKVVQRVPVKLLFEPGENAQHLL
                     VPGMSVVPIISIR"
     gene            266..1081
                     /locus_tag="VNU03_07325"
     CDS             266..1081
                     /locus_tag="VNU03_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP74308.1"
                     /translation="MKIDVLLIPVGARYPDMRAAALAAEESGFDGVWTWDHLRDPDEA
                     AEAGVPEAWTVLTALAEATRRITIGPLVLNTGLRHPGLLANMAATLQQVSGGRLLLGI
                     GAGATPSMAYAREQEALGMEVAGDRVRAERVAETAQVLRRLWSGDASSFAGSQFRLDR
                     PSGYLRPDPPPPIIVGGFGPRMAAIAGRHADGFNTQAMHPDLAGLGRVAREAHAASGR
                     DPARFGLSVFAGLSERWLREGTPERARLERAGVDRLILLTSPPYDVDTIRRRS"
     gene            complement(1120..>2064)
                     /locus_tag="VNU03_07330"
     CDS             complement(1120..>2064)
                     /locus_tag="VNU03_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWP74309.1"
                     /translation="ALMDAAVAYIVAVQEAAGIDVVSDGEWRRRSYIGIIAEIMHGFA
                     REVRDGLSWHTVVEPMTPRRPGLLGEEARFLVGRTRRATKVALPSPYLLAQRMWDPDR
                     SRAAYPTREAFMRALVPVLRAELLAVRAAGVTVAQFDDPHLCLLVDPKVRAGFADPDA
                     EAALCVDLLNEIVAGVDGIVTAVHLCRRNKARQGWIGEGGYDPILPFLRRLAVKQYVL
                     EFTIPVAGDFAVLRQLPDDRQIGLGCVDCRGAQIDSPEAIAERVGQALRHVDAERLWL
                     NPDCGFAPGSAADIPIDEAYAKLRNEAAAATLLRARFP"
BASE COUNT          275 a          729 c          778 g          282 t
ORIGIN      
        1 atccaggccg gctcgggcgc ggtgttctcg ctgctgccgc ccgagaacgc caccggcaac
       61 ttcgtgaagg tggtgcagcg cgtgccggtg aagctgctct tcgagcccgg cgagaacgcc
      121 cagcacctgc tcgtgcctgg catgtccgtc gttccgatca tctccattcg ctgatttctc
      181 cctctcccct ccggggagag ggcagggtga ggggagtaag gggtgagggg agtatagggt
      241 gaggggagta tagggtgagg ggagtatgaa gatcgacgtg ctcctgattc ccgtcggcgc
      301 gcgctatccg gacatgcgcg ccgccgcgct cgccgccgag gagtccggct tcgacggggt
      361 gtggacgtgg gaccacctgc gcgatcccga cgaggcggcc gaagccggcg tgcccgaggc
      421 gtggaccgtc ctcaccgcgc tcgccgaggc cacgcggcgg atcacgatcg gccccctcgt
      481 gctgaacacc gggctgcgcc atcccggcct gctcgccaac atggcggcca cgctccagca
      541 ggtctccggc ggccggctgc tgctcggcat cggcgcgggc gccacgccct cgatggccta
      601 cgcgcgcgag caggaagcgc tcggcatgga ggtggccggg gaccgggtgc gcgcggagcg
      661 cgtggccgag accgcgcagg tgctgcgacg gctgtggagc ggcgacgcct cgagctttgc
      721 ggggagccag ttccgcctgg accgcccgtc gggctacctg cggcccgatc cgcccccgcc
      781 catcatcgtg ggcggcttcg ggccgcgcat ggccgcgatc gcgggccgtc acgccgacgg
      841 gttcaacacc caggccatgc atccggatct ggccgggctg ggccgcgtgg cccgggaagc
      901 ccacgccgcg tcggggcgcg atcccgcgcg gttcggcctg agcgtcttcg cgggcctgag
      961 cgagcgctgg ctgcgcgagg gcacgccgga gcgggcgcgc ctcgagcggg cgggggtcga
     1021 tcggctgatc ttgctgacgt cgccgccgta cgacgtggac acgattcgac ggaggagctg
     1081 accgcccctc accctgccct ctccccaggg gggagagggt taggggaagc gggcgcgcaa
     1141 gagggtagcg gcggcggcct cgttgcgcag cttggcgtag gcctcgtcga tcgggatgtc
     1201 ggccgcgctc ccgggagcga agccgcagtc ggggttgagc cagaggcgct cggcgtcgac
     1261 gtggcgcagg gcctgcccga cgcgctccgc gatcgcctcc gggctgtcga tctgcgcgcc
     1321 gcggcagtcc acgcagccga ggccgatctg gcgatcgtcc gggagctgtc gcagcaccgc
     1381 gaagtcgcct gccaccggga tggtgaactc caggacgtac tgtttcaccg cgaggcgccg
     1441 caggaacggc aggatcggat cgtagccgcc ctcgccgatc cagccctggc gcgccttgtt
     1501 gcggcggcag aggtgcaccg cggtcacgat gccgtcgacg ccggccacga tctcgttgag
     1561 caggtccacg cagagcgcgg cctccgcgtc cgggtccgcg aagccggcgc gcaccttcgg
     1621 gtccaccagc aggcagaggt gcgggtcgtc gaactgggcc accgtgaccc cggccgcgcg
     1681 caccgcgagc agctccgcgc gcagcaccgg caccagcgcg cgcatgaacg cctcgcgcgt
     1741 ggggtacgcg gcgcgcgagc ggtccgggtc ccacatgcgc tgggccagga ggtaggggga
     1801 gggcagggcg accttggtgg cgcgccgggt gcggccgacc aggaagcgcg cctcctcgcc
     1861 gagcagtccc ggccgacgcg gcgtcatcgg ctccaccacc gtgtgccacg agagcccgtc
     1921 gcgcacctcg cgcgcgaagc cgtgcatgat ctccgcgatg atcccgatgt aggagcgccg
     1981 gcgccattct ccgtcggaga ccacgtcgat gccggccgct tcctgcaccg ccacgatgta
     2041 ggccaccgcc gcgtccatca gggc
//
LOCUS       DATMCV010000314         4352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_284480, whole genome shotgun sequence.
ACCESSION   DATMCV010000314 DATMCV010000000
VERSION     DATMCV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284480"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VNU03_07335"
     CDS             complement(<1..769)
                     /locus_tag="VNU03_07335"
                     /inference="COORDINATES: protein motif:HMM:NF025174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74310.1"
                     /translation="MKRDGSSLRFGDGASDVTVTVLSPRIVRVALAGGEATPSFVADR
                     QWAPTPFEVSDGEPLRVATSDLGIEIATDPLRLSFLDPRGEWLLREPPDGGMSAEVVD
                     GSGRRRLRACLAFSGEQHFYGLGQGGGDLDRLGVTRQIWNTQLGHGPGSDLGIPLLIS
                     NRGYGIFWDNPADAVVAVGRSDNGVRIVYAAETGALTWYFLMGGDVRGVLREAAELLG
                     RAALPPRWALGFLQSTRHFHDTDELRRLPRDIRAHRIP"
     gene            831..2222
                     /locus_tag="VNU03_07340"
     CDS             831..2222
                     /locus_tag="VNU03_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP74311.1"
                     /translation="MPTDAFVALEDLWARAGREPGALERATLTGADPILPTDFKIGTA
                     ASATIAAGALAASEIWRLRTGRGQTVSVDLRAAVAAFRSERYLRLETDPSLDRRDPVF
                     GFYRAGDGRWIQIHSNLPHHHAGALKVLGCEGTREAVAAAVAKWTAADLENAFAAAAL
                     PTGMVRSRAEWHAHPQGAAVAALPLLEIERIGEAPPEPAGEGPRALSGVRVLDLTRVI
                     AGPVCGRTLAAYGADVLLVTAPHLPNLPGLVVDTGLGKLSASLDLRQAGNLDTLQGLI
                     RGADVFCQSYRPGALAHLGLAPEEVARLRPGIVYVTLSAFGHEGPWRARRGFETIVQS
                     VSGMAHEQGIFQGGESPRHLPAQVVDHGTGYLAAFGAMIALARRAREGGSYLVRVSLA
                     QTGRWVDALGRVDGRSTPDLPLDAVQDLLADMDSPFGRLRHVIPAARFSETPARWARP
                     TVPPGTHPAAWPA"
     gene            2331..2696
                     /locus_tag="VNU03_07345"
     CDS             2331..2696
                     /locus_tag="VNU03_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74312.1"
                     /translation="MMVQPVWLKPGIWGAVIGGVLTMIVGFGWGGWTTSGTAGQMAVK
                     QADAAVTAALVPICLAGQKVDVARVKKLGELTAITSSWEQTEFVMKTGWATFPGQTDP
                     NRAVAEGCASALLKTAATK"
     gene            2834..3235
                     /locus_tag="VNU03_07350"
     CDS             2834..3235
                     /locus_tag="VNU03_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74313.1"
                     /translation="MWRRIVVAGLLGLGLVSSARALEPQAIVGDWVGEWNNGLGVHDA
                     VYMTVTKVLGDRVEGTVYWKATPGTASDNRDLVFVGTLIGSTLSVRGGPTVPGSPAMS
                     FSCSVSRDGARMDGFFQGTGRSAVSFARKNP"
     gene            3247..3720
                     /locus_tag="VNU03_07355"
     CDS             3247..3720
                     /locus_tag="VNU03_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74314.1"
                     /translation="MTSPVTEEKSYPVTPPSMTVKSGIITGEVVDLKVTERVEKGTDR
                     VVSPARLTGTLRLENTSANRTVRLVEGKLLFMDVHGQVIKLEGDRAEPIVRFTPYGDE
                     RLDPGQDTTRLLDVEFPVEALKAERLKEIRLELAYIPSPFREEVVNFVVSIGAGK"
     gene            3737..3889
                     /locus_tag="VNU03_07360"
     CDS             3737..3889
                     /locus_tag="VNU03_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74315.1"
                     /translation="MTRFIQLCASQNNLFALDEEGHVYQYHFNVRTWVKLAVTRDVQQ
                     EDGAAS"
     gene            complement(3939..>4352)
                     /locus_tag="VNU03_07365"
     CDS             complement(3939..>4352)
                     /locus_tag="VNU03_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HWP74316.1"
                     /translation="AAVEEGIVPGGGVALLRASVALDSLKLSGDEATGVDIVRRALEE
                     PLRQIVHNAGLEGSIVVEKVKSSKDASYGFDAESNQYVDMVKAGIIDPTKVERVALQH
                     AASIASLLLTTEALITDNPEEKSAMPAMPHGGGDF"
BASE COUNT          643 a         1537 c         1556 g          616 t
ORIGIN      
        1 agggaatccg atgggcgcga atgtcacggg ggagccggcg cagctcgtcg gtgtcgtgga
       61 agtgccgcgt cgactgcagg aagccgaggg cccagcgcgg cggcagcgcc gcccgtccca
      121 gcagctccgc cgcctcgcgc aggacgccgc gcacgtcgcc gcccatcagg aagtaccagg
      181 tgagcgcgcc ggtctccgcg gcgtagacga tgcgtacccc gttgtccgag cgcccgaccg
      241 ccaccaccgc gtcggccggg ttgtcccaga agatcccgta gccccggttc gagatcagga
      301 gtggaatgcc cagatccgac cccgggccgt gacccagctg cgtgttccag atctggcgcg
      361 tcaccccgag acggtcgagg tcgccacccc cttgccccag cccgtagaaa tgctgctcgc
      421 cggagaaggc caggcaggcc cggagccggc gccggccgga gccgtcgacg acctcggcgg
      481 acatgccccc gtcgggcggc tcgcggagca gccactcgcc gcgcggatcg agaaagctga
      541 ggcgaagcgg gtcggtggcg atctcgatcc cgagatcgct cgtcgcgacc cggagcggct
      601 ccccgtccga cacctcgaac ggcgtgggcg cccactgccg atcggcgacg aaggacggcg
      661 tcgcctcccc gcccgccagc gccacccgca cgatccgtgg ggacaggacc gtcaccgtca
      721 cgtccgacgc gccgtcaccg aaccgcaggc tcgatccgtc tcgcttcacg cccggcatga
      781 taccccacgg atttgacgtg cccgcgacca tctggcaggc tagcggcgcg atgccgaccg
      841 acgccttcgt cgctctcgag gacctgtggg cccgggcggg gcgtgagccg ggcgcgctcg
      901 agcgcgcgac gctcaccgga gccgacccga tcctgcccac cgacttcaag atcgggacgg
      961 cggcctccgc caccatcgcg gcgggggcgc tcgcggccag cgagatctgg cggctgcgga
     1021 ccggccgggg ccagaccgtc tccgtggacc tgcgcgccgc ggtcgccgcg ttccgaagcg
     1081 agcggtacct gcggctcgag accgacccgt cgctcgatcg tcgcgacccg gtcttcgggt
     1141 tctaccgcgc cggcgacggg cgctggatcc agatccactc gaacctgccg caccaccacg
     1201 cgggggctct gaaggtcctc gggtgcgagg ggacgcgcga ggccgtcgcg gccgcggtgg
     1261 ccaagtggac cgccgccgac ctcgagaacg ccttcgcggc cgcggccctg cccaccggca
     1321 tggtgcgctc gcgggcggag tggcacgccc acccgcaggg cgccgcggtc gccgcgctgc
     1381 cgctgctcga gatcgagcgg atcggcgagg cgccgcccga gccggcgggc gaagggcccc
     1441 gcgcgctctc gggcgtccgg gtgctcgacc tcacgcgcgt catcgcggga ccggtgtgcg
     1501 ggcgcaccct ggccgcgtac ggggccgacg tgctgctcgt caccgcgccg cacctgccga
     1561 atctccccgg cctcgtggtc gacaccggcc tcggcaagct ctcggcgtcg ctcgatctcc
     1621 gccaggcggg gaacctcgac acgctgcagg ggctgatccg cggcgccgac gtcttctgcc
     1681 agtcctatcg gccgggcgcg ctcgcgcatc tcggactcgc gccggaggag gtggcccggc
     1741 tccgtcccgg catcgtctac gtgaccctgt ccgccttcgg acacgagggg ccgtggcggg
     1801 cgcgacgggg attcgagacc atcgtccagt cggtcagcgg catggcgcac gagcagggga
     1861 tcttccaggg tggcgagtct ccgcggcacc tgcccgcgca ggtggtggac cacggaaccg
     1921 gctatctcgc cgccttcggg gcgatgatcg cgctggcccg ccgcgcccgc gaaggcggga
     1981 gctatctcgt gcgggtctcg ctggcccaga ccggtcgctg ggtcgacgcc ctcggccgcg
     2041 tggatgggcg cagcacgccc gaccttccgc tggacgcggt ccaggacctg ctcgcggaca
     2101 tggactcgcc gttcggccga ctccggcacg tcattcccgc cgcgcgcttc tcggagacgc
     2161 ccgcgcgctg ggcccgcccg accgtgccgc ccggcacgca ccccgccgcc tggcccgcct
     2221 aggcggcatc gcaccgtagt agacctaggt ccaatacccg gcccgcggcg accctcctac
     2281 actgggggtc cgattcacca attctccgag gggccaggag gtaactcatc atgatggtgc
     2341 aacctgtgtg gctgaagccg ggaatctggg gtgcggtcat cggtggcgtg ctgacgatga
     2401 tcgtggggtt cggttggggg ggctggacga cgagcggcac tgcgggccag atggcggtca
     2461 agcaggccga cgctgcggtc accgcggcgc tggtgccgat ctgcctcgcc gggcagaagg
     2521 tcgacgtcgc ccgcgtcaag aagctgggcg agctgaccgc gatcacctcg tcctgggagc
     2581 agaccgagtt cgtgatgaag accgggtggg cgaccttccc gggccagacg gatccgaatc
     2641 gagcggtggc ggaaggctgc gcgtcggcgc tgctcaagac cgccgcgacc aagtagcgcg
     2701 atagagtctc cgctccgcgc gatggcacag catcgcggcg agtggaccaa ggtccaatag
     2761 gcgtcgttgc ggcggactga tatcgtcccc tgacggaaga gcggagtcga tagccgtcag
     2821 cgggaggcgc cggatgtggc gaaggatcgt cgtggccggg ctgctggggc tgggcctggt
     2881 gtcgagcgcc cgggcgctgg agccgcaagc catcgtcggg gactgggtag gcgagtggaa
     2941 caacggcctc ggcgtccatg acgcggtata catgacggtc accaaggtgc tgggagaccg
     3001 ggtcgaaggc accgtgtact ggaaggccac cccggggacg gcctcggaca accgcgacct
     3061 ggtgttcgtg gggaccctga tcgggagcac gctgtcggtc cggggcgggc ccaccgtgcc
     3121 ggggagcccg gcgatgagct tctcctgcag cgtcagccgg gacggcgccc gaatggacgg
     3181 gttcttccag ggcaccggtc ggtctgcggt gtcgttcgcc aggaagaacc cctgaggagc
     3241 gcggtgatga catctccggt gaccgaagag aagagctatc cggtcacgcc cccgtcgatg
     3301 acggtcaagt ccgggatcat caccggagaa gtcgtcgacc tgaaggtcac cgagcgcgtc
     3361 gagaagggga ccgaccgggt ggtctcgccc gcccggctca ccgggacgct gcggctcgag
     3421 aacacctccg cgaatcggac ggtgcgcctg gtggagggca agctcctgtt catggacgtc
     3481 cacgggcagg tcatcaagct cgagggcgat cgcgccgagc cgatcgtccg gttcaccccg
     3541 tacggcgacg agcgcctcga tcccggtcag gacacgaccc ggctcctcga cgtggaattc
     3601 ccggtggagg cgctgaaggc ggagcggctg aaggagatcc gcctggagct ggcgtacatc
     3661 ccgtccccgt tccgcgagga ggtcgtcaac ttcgtggtgt cgatcggcgc gggcaagtag
     3721 gcaggaggat cagccgatga ccagattcat ccagctgtgc gcgtcgcaga acaacctctt
     3781 cgcgctcgac gaggagggcc acgtgtacca gtaccacttc aacgtcagga cctgggtcaa
     3841 gctggccgtc actcgtgacg tgcagcagga ggacggagcg gcgtcctagc cgccccgtcc
     3901 gcgcccggct cccgccgccg ggctggcgtg ttcagggtct agaagtcgcc gccgccgtgg
     3961 ggcatggccg gcatggccga cttctcctcc ggattgtcgg tgatcagcgc ctcggtggtg
     4021 agcagcaggg aggcgatcga cgcggcatgc tggagcgcca cgcgctccac cttggtcgga
     4081 tcgatgatcc cggccttcac catgtccacg tactggttcg actcggcgtc gaatccgtac
     4141 gaggcgtcct tggagctctt caccttctcg accacgatgg agccctccag ccctgcattg
     4201 tgcacgatct gacgcagcgg ctcctcgagc gcccgccgca cgatgtccac gccggtcgcc
     4261 tcgtcaccgg acagcttgag cgagtcgagg gccaccgagg cccgcagcag ggcgaccccg
     4321 ccgcccggca cgatgccttc ctccaccgcg gc
//
LOCUS       DATMCV010000315         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_882047, whole genome shotgun sequence.
ACCESSION   DATMCV010000315 DATMCV010000000
VERSION     DATMCV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882047"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1891)
                     /gene="uvrC"
                     /locus_tag="VNU03_07370"
     CDS             complement(17..1891)
                     /gene="uvrC"
                     /locus_tag="VNU03_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWP74317.1"
                     /translation="MTLEEKVDQVPARPGVYLYKDAKAQIIYIGKAASLRNRVRSYFQ
                     ESRTPDPKTDALVGQIRDLEYIVTANELEAMILESTLVKKHRPRYNIILRDDKHYPFL
                     KLTTNEEFPRLVVARRVQKDGASYFGPFYPATAMRETLRLVRQLFPLRTCRIKIDGTL
                     SRPCLQYFIHRCNAPCTGWETAEGYAKTVKDVERFLEGKSDDLAAQLTREMETAAEEM
                     KFERAAALRDRVQALHTVRERQQMISTEEEDQDVVGVVRQGSEACVQLFFVRKGRLLG
                     RESFFFERVSGWSDGEILSAFIRQFYARNVVPPPEILLSEAVPEADLTVEWLVQRRGG
                     RVELQAPQRGRKRDLVGMAEENAALALQTHLLARGNRQQVVLEDIERSLGLPGPPHRI
                     EAFDISTHQGKETVASMVVWQDGDMKKDDYKRFKIRTVVGTDDFASMQEVLTRRYGRA
                     LESEGVMPDLILLDGGRGQLGAGLKALEDLGLDYVPVAALAKRAEEVYTPDRLEPLVL
                     DMGSPALQALQKIRDEAHRFAITYHKKLRHRRAIASVLDQIPGVGPTLRTSLLKTLGS
                     AKGVREASVADLASVPKITPKLAQRIYDFFHPAPGQGEPSPGRGGLRLETEPKGGDVK
                     "
     gene            complement(1902..2117)
                     /locus_tag="VNU03_07375"
     CDS             complement(1902..2117)
                     /locus_tag="VNU03_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74318.1"
                     /translation="MLFKWVLIFFFALSIVLFVLSKAIAFMNPPRPGRPQPVLLGRVR
                     RALTWTVLVPALAALVLLAVTLLRQPP"
     gene            complement(2124..>3441)
                     /locus_tag="VNU03_07380"
     CDS             complement(2124..>3441)
                     /locus_tag="VNU03_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWP74319.1"
                     /translation="PEVRVYPASHYVTPAAQLERAIEGIMDELRERLGFLKARGKLLE
                     AQRLEQRTLFDMEMLRELGYCHGVENYSRHLSGRKPGQNPPTLMDYLPKDALVILDES
                     HVTAPQLRGMYPGDRSRKEALVEYGFRLPSAFDNRPLTFEEFVRLVPQTLYVSATPGP
                     YELEKAGVERSGVGRGLAGGTMAEQIIRPTGLMDPQITVRPASSQVDDLMAEVRARAE
                     RDERVLITTLTKRMAEDLTEYYQQNGLRVRYLHSDIDTLERVALIRDLRLGKFDGLIG
                     INLLREGLDLPEVSLVAILDADKEGYLRSATSLIQTSGRAARHVNGEVIMYADTVTGS
                     MREALGETERRRVVQGAYNQAHGITPESVRRSIRDLLESVPERDYYTVEVERTPATQW
                     ESPAALAAHVTELETAMKDAARRLDFERAAELRDRIKALRKVDLGV"
BASE COUNT          499 a         1184 c         1212 g          546 t
ORIGIN      
        1 gccatcatga ggatcttcat ttgacgtcac ctcctttcgg ttcggtctcc aaccgtaaac
       61 ccccccgccc cggggaaggt tccccctgcc ccggagccgg gtggaagaaa tcatagatgc
      121 gttgcgccag cttcggggtg attttcggca ccgaggccag atccgccacc gaggcctccc
      181 ggacgccctt ggccgaaccc agcgtcttga gcaggctggt gcgaagcgtc ggccccaccc
      241 cgggaatctg gtcgagcacc gacgcgatgg cccgccggtg acggagcttc ttgtggtacg
      301 tgatcgcgaa acggtgcgcc tcgtcccgga tcttctggag cgcctgcagc gcgggcgagc
      361 ccatgtcgag cacgagcggc tcgagccggt ccggggtgta gacctcctcg gcgcgcttgg
      421 ccagcgcggc caccgggacg tagtcgagac ccaggtcctc gagcgccttg agcccggcgc
      481 cgagctggcc gcgtccgccg tccagcagga tcaagtcggg catcacgccc tcgctctcga
      541 gcgcgcggcc gtagcggcgg gtcagcacct cctgcatcga ggcgaagtcg tcggtgccga
      601 cgacggtgcg gatcttgaag cgcttgtagt cgtccttctt catgtccccg tcctgccaca
      661 ccaccatcga ggccaccgtc tccttgccct ggtgggtgga gatgtcgaag gcctcgatcc
      721 ggtgcggcgg cccgggcagg ccgagcgagc gctcgatgtc ctccaggacc acctgctggc
      781 gattgccgcg cgcgagcagg tgcgtctgga gcgcgagtgc cgcgttctcc tcggccatgc
      841 ccaccaggtc gcgcttgcgc ccgcgctggg gcgcctggag ctccacccgg ccgccccgcc
      901 gctgaacgag ccactccacg gtcaggtccg cctcgggaac cgcctccgag agcaggatct
      961 cgggcggcgg caccacgttg cgcgcgtaga actggcggat gaacgcggag aggatctcgc
     1021 cgtcgctcca gccggagacc cgctcgaaga agaacgactc gcgcccgagg agccggccct
     1081 tgcgcacgaa gaagagctgc acgcacgcct ccgacccctg ccgcaccacc cccaccacgt
     1141 cctggtcctc ctcctcggtg gagatcatct gctgccgctc ccgcaccgtg tggagcgcct
     1201 ggacccggtc gcgcagggcc gcggcccgct cgaacttcat ctcctccgcc gcggtctcca
     1261 tctcgcgggt gagctgggcc gcgaggtcgt cgctcttgcc ctcgaggaag cgctccacgt
     1321 ccttgaccgt cttggcgtag ccctccgcgg tctcccatcc cgtgcagggg gcgttgcagc
     1381 gatggatgaa gtactgcagg cacggccgcg acagggtgcc gtcgatcttg atccggcagg
     1441 tgcggagcgg gaagagctgg cgcacgaggc gcagggtctc ccgcatcgcg gtggcgggat
     1501 agaaggggcc gaagtagctc gcgccgtcct tctgcacccg ccgggccacc accagccggg
     1561 gaaactcctc gttggtggtc agcttgagga acgggtagtg cttgtcgtcc cgcaggatga
     1621 tgttgtagcg gggccggtgc ttcttcacca gcgtcgactc gaggatcatc gcctcgagct
     1681 cgttggcggt gacgatgtac tcgagatcgc ggatctgccc gaccagggcg tcggtcttgg
     1741 ggtcgggggt gcgcgattcc tggaagtagg agcgcacgcg gttccgcagg gaggcggcct
     1801 tgccgatgta gatgatctgg gccttggcgt ccttgtagag gtagacgccc ggccgcgccg
     1861 ggacctggtc gaccttctcc tcgagggtca tcgacccgtg ctcacggggg ctggcgcagg
     1921 agcgtgaccg cgagcaggac cagcgcggcg agcgcgggca cgagcaccgt ccaggtgagc
     1981 gcgcggcgga cgcggcccag cagcaccggc tgggggcggc cgggacgcgg cggattcatg
     2041 aacgcgatgg ccttcgagag cacgaagagc acgatcgaca gcgcgaagaa gaagatcagc
     2101 acccacttga agagcatcag ccttcaaaca ccaagatcga ccttccggag ggccttgatg
     2161 cggtcgcgca gctcggcggc ccgctcgaag tcgaggcggc gcgccgcgtc cttcatcgcg
     2221 gtctcgagct cggtcacgtg ggccgcgagc gccgcggggc tctcccactg ggtcgcgggg
     2281 gtccgctcga cctcgaccgt gtagtagtcg cgctccggca ccgactccag caggtcgcgg
     2341 atcgagcggc gcaccgactc cggggtgatg ccgtgggcct gattgtacgc cccctgcacc
     2401 acgcgacggc gctcggtctc gccgagggcc tcccgcatcg agccggtcac ggtgtcggcg
     2461 tacatgatga cctcgccgtt gacgtgccgg gccgcgcggc cggacgtctg gatcagggag
     2521 gtcgcggagc gcaggtagcc ctccttgtcc gcgtccagga tcgcgaccag ggacacctcg
     2581 gggaggtcga gcccctcccg cagcaggttg atgccgatga ggccgtcgaa cttgccgagg
     2641 cgcaggtcgc ggatcagggc gacccgctcc agggtgtcga tgtcggagtg caggtagcgg
     2701 acccgcagcc cgttctgctg gtagtactcg gtgaggtcct ccgccatgcg cttggtcagc
     2761 gtggtgatca gcacccgctc gtcgcgctcc gcccgggccc gcacctcggc catgagatcg
     2821 tccacctggg aggaggcggg gcgcaccgtg atctgcgggt ccatcagccc ggtgggccgg
     2881 atgatctgct ccgccatcgt cccgcccgcg aggccccggc cgaccccgct ccgctcgaca
     2941 ccggccttct ccagctcgta gggccccggg gtcgccgaga cgtagagggt ctggggcacg
     3001 aggcgaacga actcctcgaa ggtgagcggc cggttgtcga acgcggacgg caggcggaag
     3061 ccgtactcga cgagcgcctc cttgcgcgag cgatcacccg ggtacatccc gcgcagctgg
     3121 ggcgcggtga cgtgcgactc gtcgaggatc accagcgcgt ccttggggag gtagtccatc
     3181 agggtgggcg ggttctgccc cggcttgcgc ccggagaggt ggcgcgagta gttctcgacc
     3241 ccgtggcagt agccgagctc gcgcagcatc tccatgtcga agagggtgcg ctgctcgagc
     3301 cgttgcgcct ccaggagctt gccgcgcgcc ttgaggaacc cgagccgctc gcgtagctcg
     3361 tccatgatgc cctcgatcgc ccgctcgagc tgggccgcgg gggtcacgta gtggctcgcc
     3421 gggtagacgc ggacctcggg c
//
LOCUS       DATMCV010000316         4193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_15429, whole genome shotgun sequence.
ACCESSION   DATMCV010000316 DATMCV010000000
VERSION     DATMCV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15429"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VNU03_07385"
     CDS             complement(<1..943)
                     /locus_tag="VNU03_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP74320.1"
                     /translation="MATTARAAVFFGPGKPFEIKQVPIPEIEPDGVLIRVTHANICGS
                     DLHFWRGDAPLRLPDDGWIFGHEMTGRVARLGARVKTDSLGRALKEGDRVAYTYFYPC
                     GRCYACVHEERAACPNKIERPLGPSAPPHLHGAFADHYYLRPGGALFTVPDVLSDEAV
                     AGVNCALSQVFYGLHVAGVRQGDRVVIQGAGGLGIQAAAVAKDMGAETVIVIDQIPGR
                     LELARAFGADHAINVKEVTDRRERVKLVRQWTGGVGADVACDLVGFPAVIPEGIEMLR
                     SGGTYLEIGTISRGAKIELEPSLLVWGSKKIVGVIQYE"
     gene            complement(946..2175)
                     /locus_tag="VNU03_07390"
     CDS             complement(946..2175)
                     /locus_tag="VNU03_07390"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWP74321.1"
                     /translation="MAQLIVQKYGGSSVADPEKIKNVARRVAGYAREGHRLVVVVSAM
                     GKTTDGLLALASAITPTPDPREMDMLLATGEQVTIGLLAMALQALGQPACSFTGAQIG
                     LVTDQAHTRARIKRISAERIHAALDAGRVAVVAGFQGTTEDGEITTLGRGGSDLTGVA
                     LAAALKADVCEIFTDVDGVYTADPNVVPDAHKLPRISYDEMLEMAALGAKVLQARSVE
                     FAKKYNVPVHVRSTFKPDPGTLVTREDHSMEDVVVTGITHDRGQAKVSILRVPDRPGI
                     ASRVFGGLGKENIVVDMIVQNISREGLTDISFTLPRADRARAEGVLGALAREIGAAGV
                     VADDRVAKISILGVGMRSHSGVAARMFETLSGEGINIQMISTSEIALSCVIEDKYAEL
                     AVRALHDAFALGAERGG"
     gene            complement(2226..2852)
                     /locus_tag="VNU03_07395"
     CDS             complement(2226..2852)
                     /locus_tag="VNU03_07395"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HWP74322.1"
                     /translation="MTYRTALVLALLALPAGPAAAQAPPPPPAAPGAPAAPGAPIQGL
                     ERLSPEERAVAERNLERWRSMTPEQQQRAMDNYRHWRSLSPEERQSARQNLQRLRQMP
                     PGERARVMQDFQRWNQLPDERKRELERAYQRYQQLPPERQRQIQQRFERYQSLSPEQR
                     QRMDRNLERWRSMTPEERQRAREQVREKRQERQKEIRPREDAPGRRPR"
     gene            complement(2854..3165)
                     /locus_tag="VNU03_07400"
     CDS             complement(2854..3165)
                     /locus_tag="VNU03_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74323.1"
                     /translation="MRAWLWPAGLLVMTLAGTIEVGAADRGATTKRAGAPEAVDAEML
                     RDLDLLSSPDYTRDREVARRMGLLERMRMLEAQRAQEAAGGATGASAPEPPPPPARDG
                     R"
     gene            complement(3169..3663)
                     /locus_tag="VNU03_07405"
     CDS             complement(3169..3663)
                     /locus_tag="VNU03_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWP74324.1"
                     /translation="MTDAHVDELLVDHLRGDLEGPERARVDAHLAACAECRASREGFA
                     RIVAALADTAPPAPPIHWGAYRAELRERIERAGAGRGGRWAWLARPVPALVAAALVAA
                     LVVAGLPGLRGPGAPDPLALDNTILASRLDMIARLDVVQDLDLLEDFNVINELDELPG
                     PSKS"
     gene            complement(3660..>4193)
                     /locus_tag="VNU03_07410"
     CDS             complement(3660..>4193)
                     /locus_tag="VNU03_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP74325.1"
                     /translation="KPGAFDLLVERYQERAYRIAWSVVRDREEARDCSQEAFLRLHES
                     AGSFAGQSKFSTWFYRILVNCCLDQKRRGRGWRQLLGWRDRRGDGPDEPDPVEQAPAP
                     FADPADHVDAERRMSQVWALVEELSPQQRAAVTLSCREGMATKDIAAVLSLSEATVRV
                     HLHRALATLRRRIGDQA"
BASE COUNT          541 a         1523 c         1523 g          606 t
ORIGIN      
        1 cctcgtactg gatcaccccg acgatcttct tggagcccca caccaggagc gacggctcga
       61 gctcgatctt ggctccgcga ctgatggtgc cgatctcgag ataggtgccg cccgagcgca
      121 gcatctcgat gccctcgggg atcaccgcgg gaaagccgac caggtcgcac gccacgtcgg
      181 ccccgacccc gccggtccac tggcgtacca gcttcacccg ctcgcgccgg tccgtgacct
      241 ccttgacgtt gatggcgtgg tcggccccga aggcgcgggc cagctcgagc cgccccggga
      301 tctgatcgat cacgatcacg gtctcggcgc ccatgtcctt ggccaccgcg gcggcctgga
      361 tgccgagccc acccgcgccc tggatcacca cccggtcgcc ctgccgcacc ccggccacgt
      421 gcaggccgta gaagacctgg gagagcgcgc agttcacgcc ggccaccgcc tcgtcggaga
      481 gcacgtccgg caccgtgaac agcgcgccgc cggggcggag gtagtagtgg tcggcgaagg
      541 cgccgtggag atgcggcggc gcgctcggac cgagcggccg ctcgatcttg ttggggcagg
      601 ccgcgcgctc ctcgtgcacg cacgcgtagc agcggccgca ggggtagaag taggtgtagg
      661 cgacgcggtc gccctccttg agcgcgcgcc cgagcgagtc ggtcttcacc cgcgcgccga
      721 gccgggccac ccgcccggtc atctcgtggc cgaagatcca gccgtcgtcg ggcaggcgca
      781 ggggcgcgtc gccacgccag aagtggaggt cggagccgca gatgttggcg tgggtcaccc
      841 ggatcagcac gccgtccggc tcgatctccg ggatcggcac ctgcttgatc tcgaacggct
      901 tgccggggcc gaagaagacg gcggcgcgcg cggtggtcgc catggtcagc ctccccgctc
      961 cgcgccgagg gcgaaggcgt cgtgcagcgc ccgcaccgcc agctcggcgt acttgtcctc
     1021 gatcacgcag gagagcgcga tctcggaggt ggagatcatc tggatgttga tgccctcgcc
     1081 ggacagggtc tcgaacatgc gcgccgcgac ccccgagtgg ctccgcatcc cgacgcccag
     1141 gatggagatc ttggccaccc ggtcgtcggc gaccacgccc gccgcgccga tctcgcgggc
     1201 gagagcgccc agcacgccct ccgcgcgggc gcggtccgcg cgaggcaggg tgaacgagat
     1261 gtcggtgagc ccctcgcggc tgatgttctg cacgatcatg tcgaccacga tgttctcctt
     1321 gccgaggccg ccgaacaccc gcgaggcgat gccgggccgg tcgggcacgc gcaggatcga
     1381 gaccttggcc tgcccgcgat cgtgggtgat gccggtcacc accacgtctt ccatgctgtg
     1441 atcctccctg gtcaccagcg tgcccgggtc cggcttgaag gtggagcgga cgtggaccgg
     1501 gacgttgtac ttcttggcga actcgaccga gcgggcctgc aagaccttgg cccccagggc
     1561 ggccatctcc agcatctcgt cgtaggagat gcgcggcagc ttgtgggcgt cgggcaccac
     1621 gttgggatcg gcggtgtaga cgccgtccac gtcggtgaag atctcgcaca cgtcggcctt
     1681 gagcgcggcg gccagcgcga cgccggtgag atcggagccg ccgcggccga gggtcgtgat
     1741 ctccccgtcc tcggtggtgc cctggaagcc ggccaccacc gcgacacggc ccgcgtccag
     1801 cgccgcgtgg atccgctccg cgctgatgcg cttgatgcgg gcacgagtat gcgcctggtc
     1861 ggtgacgagg ccgatctgcg cgccggtgaa ggagcacgcg ggttggccga gcgcctgcag
     1921 ggccatcgcg aggaggccga tggtgacctg ctcgccggtg gcgagcagca tgtccatctc
     1981 gcgcgggtcg ggggtcgggg tgatcgcgga ggcgagcgcg agcagcccgt cggtggtctt
     2041 gcccatcgcg gagaccacga ccaccagacg gtgtccctca cgcgcatagc ccgccacacg
     2101 ccgggccacg ttcttgatct tctcggggtc ggcgaccgac gagccgccgt acttctggac
     2161 gatcagctgc gccatgagag ggccatcgta cacgaccccc gcgcgcagtg taaagtcgcg
     2221 ccccttcagc gagggcgacg tcccggcgcg tcctcgcgcg ggcggatctc tttctggcgc
     2281 tcctgccgct tctcgcggac ctgctcgcgc gcgcgctggc gctcctcggg ggtcatgctc
     2341 cgccagcgct cgaggttccg gtccatccgc tggcgctgct cggggctcag cgactggtag
     2401 cgctcgaagc gctgctggat ctgccgctgc cgctccgggg ggagctgctg gtagcgctgg
     2461 taggcgcgct ccagctcgcg cttccgctcg tccggcagct ggttccagcg ctggaagtcc
     2521 tgcatgaccc gcgcgcgctc gccggggggc atctggcgga ggcgctggag gttctgacgg
     2581 gcgctctggc gctcctccgg ggagaggctg cgccagtggc ggtagttgtc catcgcgcgc
     2641 tgctgctgct cgggagtcat gctgcgccac cgctcgaggt tgcgctcggc cactgcgcgc
     2701 tcctccggcg agaggcgctc gagcccctgg atcggcgcgc cgggcgccgc gggcgcgcct
     2761 ggggccgcgg gaggcggggg cggagcctgg gccgccgcgg ggccggcggg cagcgccagc
     2821 agtgccagga cgagcgcggt ccggtatgtc atgtcagcgg ccgtcccggg ccggaggcgg
     2881 agggggctcg ggggcggacg caccggtggc tccgcccgcg gcctcctgcg cgcgctgggc
     2941 ttcgagcatg cgcatgcgct cgaggaggcc catgcggcgg gccacctcgc ggtcccgggt
     3001 gtagtccggg ctgctcagca gatccagatc tctcagcatc tccgcgtcca ccgcctccgg
     3061 cgcgccggcg cgcttcgtcg tcgcgcctcg atcggccgcg cccacctcga tcgttcccgc
     3121 cagcgtcatc acgagcaggc cggcgggcca gagccatgcc ctcacggctc agctcttgct
     3181 cggccccggc agctcgtcca gctcgttgat gacgttgaag tcctcgagca ggtcgaggtc
     3241 ctgcaccacg tcgagccggg cgatcatgtc gagccggctc gcgaggatcg tgttgtcgag
     3301 cgcgagcgga tcgggcgcgc cgggtccgcg caggccgggc agcccggcca ccaccagcgc
     3361 ggccaccagg gccgccgcga ccagcgccgg caccggtcgg gcgagccagg cccagcgccc
     3421 gccgcgcccg gcaccggcgc gctcgatgcg ctcgcgcagc tcggcccggt acgcgcccca
     3481 gtggatgggg ggcgccggtg gcgcggtgtc ggccagcgcg gccacgatcc gcgcgaagcc
     3541 ctcgcgggag gcgcggcact ccgcgcacgc ggccaggtgc gcgtcgaccc gggcccgctc
     3601 cggtccctcg aggtcgccgc ggaggtgatc gaccagcagc tcgtccacgt gcgcgtcggt
     3661 catgcctgat ctccgatccg ccgcctgagc gtggcgagcg cgcggtgcag gtgtacccgc
     3721 accgtggcct ccgaaaggct cagcacggcg gcgatgtcct tggtcgccat tccctcgcga
     3781 caggagagcg tcaccgccgc gcgctgctgc ggcgagagct cttccaccag cgcccacacc
     3841 tggctcatgc gtcgctccgc gtccacgtga tccgcggggt ccgcgaacgg cgcgggcgcc
     3901 tgctcgaccg gatccggctc gtcgggcccg tcgccgcgcc ggtcgcgcca gcccagcagc
     3961 tggcgccagc cccgcccgcg acgcttctgg tcgaggcagc agttgaccag gatgcggtag
     4021 aaccacgtcg agaacttgga ctgcccggcg aaggagccgg ccgactcgtg caggcgaagg
     4081 aacgcttcct gggagcagtc gcgggcttcc tcccgatcgc gcacgacgga ccaggcgatg
     4141 cggtaggccc gctcctgata gcgctcgacc aggaggtcga aggcccccgg ctt
//
LOCUS       DATMCV010000317         3747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_951618, whole genome shotgun sequence.
ACCESSION   DATMCV010000317 DATMCV010000000
VERSION     DATMCV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951618"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..777)
                     /locus_tag="VNU03_07415"
     CDS             complement(16..777)
                     /locus_tag="VNU03_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74326.1"
                     /translation="MSEPGTGLVLETRGLSKSFGGVRAVSSVDLAMPKGEIRALIGPN
                     GAGKTTFFNMLTGQLRADAGSVLFKGERLSGLPPYAVWRRGVSRTFQITATFATLSAL
                     DNVRVARLSHAGKTYSLLEAAARLQADEARALLDQVGLVDQADRVAAVLAYGDLKKLE
                     LAVALANDPEVLLLDEPTAGMAPAERGALMALTERIARARGLTVLFTEHDMDVVFAVA
                     DRIMVLHQGRVIAEGAPAAVRADALVQRVYLGEDE"
     gene            complement(774..2600)
                     /locus_tag="VNU03_07420"
     CDS             complement(774..2600)
                     /locus_tag="VNU03_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74327.1"
                     /translation="MSLLLVQLLSGLANAMFLFLIASGLSLIFGVTRIVNFAHGSFYM
                     LAAYLTYSLAAMLPGGPAAFYLAALLAALAVAAIGGLVEVLLLRRVYRAPELYQLLLT
                     FALVLVTADLVRYLWGADNKTGPAAPGLAGSVPIAGQLFPSYDLAIILFGPLVALGLW
                     LLFHRTRWGVLIRAATQDREMVAALGVDQSRLFTSVFVLGSFLAGLGGALQVPRLALT
                     TVMDTSVIVEAFVVVVIGGMGSVWGALLASLLIGVLNAYGVLLLPKAAIVLIFVVMAV
                     VLVVRPWGLLGRPEIQLRPPGSGTVADRPALSLHPGWTVAALVVLAALPLALPTFWIS
                     IAVEIFAFALFAASLHLLMAVGGMVSFGHAAYFGLGAYGAALLLKLAGLPMPVAFVVA
                     PLVAALAAAVFGYFCVRLTSIYFAMLTLAFAQIVYAIVHQWDDVTGGDNGVLSVWPAS
                     WLATPGRYYYWALVATVAGIVLLRRVAASPFGLTLRAARDHARRAEALGVNIRALQWT
                     AFVVAGFVAGIGGAVFAFLKGSVFPVYTESPMSVQPLVMVLLGGIASPSGPLIGAAVY
                     KLLDTVITRYTDYWQLVLGAILVVLVLAFPRGIAGVLDGRRP"
     gene            complement(2673..>3747)
                     /locus_tag="VNU03_07425"
     CDS             complement(2673..>3747)
                     /locus_tag="VNU03_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74328.1"
                     /translation="YRAGVEMAVEEINAKGGVLGRKIEVLFRDDKGQPAEAVKHAQEL
                     VESDKVAMITGSFLSNVGLAVSDWAKQNKTMFLAAESLTEALTWSKGHDYAVRLRPNT
                     YEQGRMLADKAGKMKYVKWATIGPNYEYGKRAWETFRDRLKELKPDVQVVGEHWPTLG
                     KIEPGPLVTAILNQNPDALYVSLFGSDWLAFVREAGKRGLFQKMFVVGILLGEPEYID
                     PLKLEAPEGMLVTGYPWYDVKIAGHQEWVARYVKRGDKNPVLGSLIGYVTYLSVAEAL
                     KKAGAPETPKLVAAFKGLKVETPMGPVTFRASDGQSTMGAWVGTTKLDAQRGVGIMVN
                     HELIPGEKVLPSDDEVKKLRPAN"
BASE COUNT          615 a         1319 c         1343 g          470 t
ORIGIN      
        1 gcggccacgg cggcctcact catcctcgcc gaggtagacg cgctggacga gggcgtcggc
       61 gcggacggcc gcgggggcgc cctcggcgat cacccgcccc tggtggagca ccatgatgcg
      121 gtcggcgacc gcgaagacca cgtccatgtc gtgctcggtg aagagcacgg tgagcccgcg
      181 cgcccgcgcg atgcgctcgg tgagggccat gagggccccg cgctcggcgg gggccatgcc
      241 cgcggtgggc tcgtcgagca ggagcacctc ggggtcgttg gcgagcgcca ccgccagctc
      301 gagcttcttg agatcgccgt aggccaggac cgcggcgacc cggtccgcct ggtcgaccag
      361 gcccacctgg tcgagcagtg cgcgcgcctc gtccgcctgc agacgggcgg ccgcctccag
      421 gagggaatag gtcttgcccg cgtgcgagag gcgcgcgacc cggacgttgt cgagcgcgga
      481 gagcgtggcg aaggtggcgg tgatctggaa cgtccggctc actccgcgac gccagaccgc
      541 gtacggcggt agcccgctga ggcgctcgcc cttgaagagc accgatcccg cgtcggcccg
      601 gagctggccg gtgagcatgt tgaagaacgt ggtcttgccg gccccgttgg ggccgatcag
      661 ggcgcggatc tcccccttcg gcatcgcgag gtcgaccgag ctgaccgccc ggaccccgcc
      721 gaaggacttg gagagtccgc gcgtctccag caccagcccc gtcccgggct cgctcacggt
      781 cgccggccgt cgagcacgcc cgcgatgccc cgcgggaacg cgagcaccag caccaccagg
      841 atcgcgccca gcacgagctg ccagtagtcg gtgtagcgcg tgatcacggt gtcgagcagc
      901 ttgtagaccg ccgcgccgat gagcggaccg gagggcgacg cgatgccgcc caggagcacc
      961 atcaccagcg gctgcaccga catcggcgac tcggtgtaga ccgggaacac gctgcccttg
     1021 aggaacgcga agaccgcccc gccgatcccg gccacgaagc ccgccaccac gaacgcggtc
     1081 cactgcagcg cccggatgtt cacccccagc gcctcggcgc gccgggcgtg atcgcgggcc
     1141 gcgcgcaggg tgagcccgaa gggcgaggcc gcgacccggc gcagcagcac gatgccggcc
     1201 acggtggcca ccagggccca gtagtagtag cggccgggag tcgcgagcca ggacgcgggc
     1261 cagacgctca gcaccccgtt gtccccgccg gtgacgtcgt cccactggtg gacgatcgcg
     1321 tagacgatct gggcgaaggc cagggtgagc atcgcgaagt agatgctggt gagccgcacg
     1381 cagaagtagc cgaacacagc ggcggcgagc gcggccacga gcggggccac cacgaaggcg
     1441 accggcatgg gcagacccgc gagcttgagg aggagcgcgg ccccgtaggc gccgaggccg
     1501 aagtacgccg cgtgcccgaa ggacaccatg ccgcccaccg ccatcagcag gtgcaggctc
     1561 gccgcgaaga gcgcgaacgc gaagatctcc accgcgatcg agatccagaa ggtcggcagc
     1621 gcgagcggca gggcggccag gaccacgagc gccgcgacgg tccagccggg atggaggctc
     1681 agcgccgggc ggtcggccac cgtcccgctc cccggcggcc ggagctggat ctcgggccgg
     1741 ccgaggaggc cccacggccg caccaccagc accaccgcca tcaccacgaa gatgagcacg
     1801 atggccgcct tcggcagcag cagcaccccg taggcgttga gcaccccgat cagcagcgag
     1861 gccagcagcg ccccccacac cgagcccatg cccccgatca ccaccaccac gaaggcctcc
     1921 acgatcaccg aggtgtccat cacggtggtg agggcgagcc gcggtacctg aagcgcgccg
     1981 ccgagcccgg ccaggaacga gccgagcacg aagacggagg tgaagagccg cgactggtcg
     2041 acgccgagcg cggccaccat ctcgcggtcc tgggtggccg cccggatcag tacgccccag
     2101 cgggtgcggt ggaagagcag ccagaggccg agggccacca gcggcccgaa caggatgatc
     2161 gccagatcgt aggaggggaa gagctggccc gcgatcggca ccgacccggc cagccccggc
     2221 gcggcggggc cggtcttgtt gtcggcgccc cagaggtagc ggacgaggtc ggcggtcacc
     2281 aggacgagcg cgaaggtgag caggagctgg tagagctcgg gcgcgcggta gacgcggcgg
     2341 aggagcagca cctccaccag ccccccgatc gcggccaccg ccagcgcggc gaggagcgcg
     2401 gccaggtaaa aggcggccgg gccgcccggc agcatcgcgg cgagcgaata ggtcaggtac
     2461 gcggccagca tgtagaagga gccgtgcgcg aagttgacga tgcgggtgac gccgaagatg
     2521 agcgagaggc ccgacgcgat gaggaacagg aacatcgcgt tcgcgagccc gctcaggagc
     2581 tgtacgagga ggagggacat gcggtcgaag gtcccctcac cctgccccct cccccgcggc
     2641 gggggagaga gtgatcacga ggggcggggc gctcagttgg ccgggcggag cttcttcact
     2701 tcgtcgtcgg acggcagcac cttctcgccc gggatcagct cgtggttgac catgatgccg
     2761 acgccccgct gcgcgtcgag cttggtcgtg cccacccacg cgcccatcgt cgactggccg
     2821 tcggaggcgc ggaaggtgac cggccccatc ggggtctcga ccttcaggcc cttgaaggcg
     2881 gccaccagct tcggggtctc gggcgcgccc gccttcttga gcgcctccgc caccgagaga
     2941 taggtgacgt agccgatcag cgagcccagc accgggttct tgtccccgcg cttgacgtag
     3001 cgggcgaccc actcctggtg gccggcgatc ttcacgtcgt accacggata gccggtgacc
     3061 agcatgccct cgggggcctc gagcttgagg gggtcgatgt actcgggctc gccgagcagg
     3121 atgcccacca cgaacatctt ctggaagagc ccgcgcttgc ccgcctcgcg cacgaaggcg
     3181 agccagtcgc tgccgaagag cgagacgtag agcgcgtcgg ggttctggtt caggatcgcg
     3241 gtcacgagcg ggccgggctc gatcttgccg agggtcggcc agtgctcgcc caccacctgc
     3301 acgtccggct tcagctcctt gagccggtcc cggaacgtct cccacgcgcg cttgccgtac
     3361 tcgtagttgg gcccgatggt ggcccacttg acgtacttca tcttgccggc cttgtcggcc
     3421 agcatccgcc cctgctcgta ggtgttcggg cgcaggcgca ccgcgtagtc gtgtcccttg
     3481 gaccaggtga gcgcctcggt gagcgactcg gcggcgagga acatggtctt gttctgcttg
     3541 gcccagtcgg agaccgcgag ccccacgttg gagaggaagc tcccggtgat catcgcgacc
     3601 ttgtccgact ccaccagctc ctgggcgtgc ttgaccgcct ccgcgggctg gcccttgtcg
     3661 tcgcggaaca gcacctcgat cttgcgaccg agcacgcctc ccttggcgtt gatctcctcg
     3721 acggccatct cgacgccggc gcggtac
//
LOCUS       DATMCV010000318         3433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_549657, whole genome shotgun sequence.
ACCESSION   DATMCV010000318 DATMCV010000000
VERSION     DATMCV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549657"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VNU03_07430"
     CDS             <1..344
                     /locus_tag="VNU03_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74329.1"
                     /translation="DPGESGALRHARGGALTTMEPESLRRLIREKLADGRLPQNSIPR
                     LWGGAGNGETCDACEEIISKTQFVMEGVSTTGGKGIQFHVGCLHLWDSERCAPGREDT
                     SAPLPKYGPGE"
     gene            447..908
                     /locus_tag="VNU03_07435"
     CDS             447..908
                     /locus_tag="VNU03_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74330.1"
                     /translation="MITTLRHLRTLNIALLAAGTLAACAQLPSSQQPVQASNPTVTYS
                     YRTDQELLRASQNATTYCGQYQTAPRAATITNNADGSKTAVFECVRTAAVAPPPVNPN
                     LSYTYRTDQELVQASQTASAYCLRNGSQSTTSSNMMANSDGTRTVTFQCSR"
     gene            complement(1202..2140)
                     /locus_tag="VNU03_07440"
     CDS             complement(1202..2140)
                     /locus_tag="VNU03_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWP74331.1"
                     /translation="MSGVTIGVDVGGTTVAAGLVTADGLVLEHAQAPTHAHGAGRALE
                     TIVELLDRLRDGARARGVAVTGVGVGVPGTVDAEQGVVGVDVNYAPELASLPLAERLR
                     SRVEVPVFVDNDVNALVLAEWMWGAGRGARSLVMLTLGTGVGGGIVLDGHLHRGAAGS
                     AGELGHVPIDFNGPPCVCGGRGCFKSYVSGTDIAQRAEERLGRPVAAAEVFRLAAAGQ
                     PEAEAIVEEACRALGAGLAVIVNGLNPERLLLAGSVAKSLRPLEARIRASLADYAFAA
                     AIAATRLEILSLDKDATVRGGAALVRYETNRRKTPR"
     gene            complement(2153..2911)
                     /locus_tag="VNU03_07445"
     CDS             complement(2153..2911)
                     /locus_tag="VNU03_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWP74332.1"
                     /translation="MKPARSLIVNADDYGLTAGVSRGIIDAHRRGIVTSTTLLVNRPV
                     DPALIEALQSSGLGVGLHMNLTLGPPVAPAKRVASLLDGEGRFVRDAREAARQARPDE
                     ARIELGMQIDEFRRIMGRFPTHLDSHHHVGRQEPILELVLDFARAIKVPVRTQDPEVR
                     AMARKLALKTPDHFMGESGPEAYWSSERVLGHLRELPGGVTEFMTHPGYYDDELSYSR
                     YGRQRETEMTGLTDPAARALIEREGIRLIHFGNL"
     gene            complement(2908..>3433)
                     /locus_tag="VNU03_07450"
     CDS             complement(2908..>3433)
                     /locus_tag="VNU03_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74333.1"
                     /translation="VIATDVVEAAVRCARENAALNGVADRVEVRLGDGFDPVRGLAFD
                     LICTSPPQMPTPPDRERSDAMAAADNGGRDGWSLLDRVIAEAPAHLRPGGRLVFTLFG
                     FLGIKAALGKLADVGLEPAILGQETQGFPRIGYERLEHIRALDVEGTLPRSGWPATVE
                     RYVVQGTKAREDSR"
BASE COUNT          517 a         1255 c         1187 g          474 t
ORIGIN      
        1 gagaccccgg tgaatctggc gccctccgac atgccagagg aggagccttg accaccatgg
       61 aaccggaatc ccttcgtcgc ctcatccgtg agaagctcgc cgacggtcgc ttacctcaga
      121 acagcatccc gcgcctctgg ggtggtgcgg gcaacggcga gacctgtgac gcctgcgagg
      181 aaatcatctc caagacgcag ttcgtcatgg aaggcgtcag cacgacgggc ggcaagggca
      241 ttcagtttca tgtcgggtgc ctccacctgt gggactcgga gcgctgcgca cccggccgag
      301 aggacaccag cgcaccgctc ccgaagtacg gtcccggcga gtagcgcggc ggtcaccgct
      361 cgagcgccat tggacctagg tccgatacct tccccgctcg cgacgcgcta ccgtggtagg
      421 cacaccatca gccgggaggc ctgctcatga tcacgaccct tcgtcatttg cgcaccttga
      481 acatcgcgtt gctcgccgcg ggaacgctcg ccgcctgcgc ccagctgccc tcgtcgcagc
      541 aaccggtgca agcgagcaac cccaccgtca cctatagcta tcggaccgac caggagctgc
      601 tccgagcgag ccagaacgcg accacgtatt gcggtcagta ccagacggcc ccgcgcgcgg
      661 cgaccatcac gaacaacgcg gacggcagca agaccgcggt cttcgaatgc gtgcggaccg
      721 ccgccgtcgc gccgccgccg gtcaatccca acctgagcta cacgtatcgg acggaccagg
      781 agctggtgca agcatcacag accgccagcg cctactgcct gaggaacggc tcgcagtcga
      841 cgacctcgag caacatgatg gccaactcgg acgggacccg gaccgtgacg ttccagtgct
      901 cgcgttgagg ggagcgcggc cgtcatgacg acctcgttac tctcacctga gcagctgagc
      961 cagcagatac gggttcggct cgcccaggag cggctgcccg gcgccaatgg cgtgtacaag
     1021 acgcgccgag gcacgagccg gccgtgcatc atatgccgtc gcgcgatcga ggcaaccgac
     1081 atggcatttg aagtcgccga cgcaggtgtc gtcctgaccg cgcacgaggc gtgttacatg
     1141 ctctggcggg agcagtcggt atcgagcctc gcaccgaggc gaccgtagcg agagatcggc
     1201 ttcatcgagg cgtctttcgt cggttggttt cgtagcgcac gagggccgcg ccgccccgca
     1261 cggtcgcgtc cttgtcgagg ctcaggatct cgaggcgggt cgcggcgatc gccgccgcga
     1321 acgcatagtc ggcgaggctc gcccggatcc gcgcctcgag cgggcggagc gacttggcga
     1381 cgctgcccgc cagcagcagc cgctccgggt tgagcccgtt cacgatgacg gccagcccgg
     1441 ccccgagggc ccggcaggcc tcctccacga tcgcctccgc ctccggctga ccggccgcgg
     1501 cgagccggaa aacctcggcg gcggcgacgg gccggccgag ccgctcctcc gcccgctgcg
     1561 cgatgtcggt gccggagacg tagctcttga agcagccccg gccgccgcac acgcagggcg
     1621 gcccgttgaa gtcgatcggc acgtgaccga gctcgccggc gctgccggcc gccccgcggt
     1681 gcaggtggcc gtcgagaacg atgccacccc ccacgccggt gccgagggtc agcatgacca
     1741 gggaccgcgc gccgcggccc gcgccccaca tccattcggc gaggaccagc gcgttgacgt
     1801 cgttgtcgac gaacaccggg acctccaccc gcgagcggag acgctcggcg agcggcagcg
     1861 aggccagctc gggcgcgtag ttgacgtcca cgcccaccac tccctgctcg gcgtccacag
     1921 tgccgggcac cccgacgccg acgccggtca ccgcgacgcc gcgcgcccgg gcgccgtccc
     1981 ggagccggtc gagcagctcc acgatggtct cgagcgcgcg gccggcgccg tgcgcgtggg
     2041 tgggcgcctg ggcgtgctcg agcacgagcc cgtcggcggt cacgaggccg gccgcgacgg
     2101 tggtgccgcc cacgtccacc ccgatcgtga cgccgctcac cgccgggacg ccctacaggt
     2161 tcccgaagtg gatgagccgg atcccttccc gctcgatgag ggcgcgcgcc gccggatcgg
     2221 tgagcccggt catctcggtc tcccgctggc ggccgtagcg gctgtaggag agctcgtcgt
     2281 cgtagtagcc ggggtgggtc atgaactcgg tgacgccgcc gggcagctcg cgcaggtggc
     2341 cgagcacgcg ctcggacgac cagtaggcct ccggccccga ctcgcccatg aagtggtcgg
     2401 gggtcttgag cgcgagcttc cgggccatcg cccgcacctc tggatcctgg gtccgcaccg
     2461 ggaccttgat ggcccgcgcg aagtcgagca ccagctcgag gatgggctct tgccggccca
     2521 cgtggtggtg cgaatcgagg tgggtcggga agcggcccat gatccggcgg aactcgtcga
     2581 tctgcatccc gagctcgatc cgcgcctcgt ccggccgggc ctgtcgcgcc gcctcgcgcg
     2641 cgtcccgcac gaagcgcccc tctccgtcga gcagcgacgc cacccgcttc gcgggcgcga
     2701 ccggcggccc cagcgtgagg ttcatgtgca ggccgacgcc cagccccgag gactggagcg
     2761 cctcgatcag cgcgggatcc accggccggt tgacgagcag cgtggtgctg gtcacgatgc
     2821 cgcggcggtg cgcgtcgatg atgccgcggc tcaccccggc ggtgaggccg tagtcgtcgg
     2881 cgttgacgat gagcgagcgg gccggcttca tcgcgaatcc tccctggcct tggtgccctg
     2941 gacgacgtag cgctcgaccg tcgcgggcca tccgctccgc ggcagggtgc cctccacgtc
     3001 gagcgcgcgg atgtgctcga gccgctcgta tccgatccgg gggaagccct gggtctcctg
     3061 gccgaggatc gccggctcga gcccaacatc ggcgagcttg ccgagcgcag ccttgatgcc
     3121 gaggaacccg aagagcgtga agacgaggcg gccgccgggc cggaggtgcg cgggcgcctc
     3181 cgcgatcacc cggtcgagca ggctccaccc gtcccggccg ccattgtcgg cggcggccat
     3241 cgcgtcgctc cgctcgcggt cggggggcgt cggcatctgg ggcgggctcg tgcagatgag
     3301 atcgaacgcg agcccgcgca ccgggtcgaa gccgtcgccg agccggacct cgacccggtc
     3361 ggccaccccg ttgagcgccg cgttctcccg cgcgcagcgc accgccgcct ccaccacgtc
     3421 ggtggcgatc acc
//
LOCUS       DATMCV010000319         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_419230, whole genome shotgun sequence.
ACCESSION   DATMCV010000319 DATMCV010000000
VERSION     DATMCV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419230"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VNU03_07455"
     CDS             complement(<1..838)
                     /locus_tag="VNU03_07455"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWP74334.1"
                     /translation="MPIPLSRPPLDDEIKAAVLAAIDSRQYILGPECTAFEEEFARYT
                     GSRHAVVSSSATAALWMALRAFGVKPGDEVLVPSHTAFPTVEAVCFAGGVPIFVDVDE
                     SYTVDLTDAAAKITPRTVGFVPVHLYGHPADLPGIGQLCAERGLWLLEDCAQAHGATW
                     QGRKVGGFGRAGAFSFYPSKNLTVMGDGGLLVTDDDDVAARCRRLRDHGRRSKDVHLE
                     IGFNLRFNDIQAAAGRVLLRRLDAINDHRRHLASRYHAGLSGLPLALPGERVGARHVY
                     HLY"
     gene            complement(1308..2453)
                     /locus_tag="VNU03_07460"
     CDS             complement(1308..2453)
                     /locus_tag="VNU03_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWP74335.1"
                     /translation="MRLAARSIAGRTVTVRNLVAALRAGASIIAVPAALRDRAVERAL
                     AHMPALAAAVRWLEPGSRLPAGPPDQPWLLVPAASLVHTRTLQSLIAGAATSDRVIMT
                     TSPLDPAPVAILPRAIVGALWSRLASGSPVGPDLARLLKEWGAQPRASTDPFVAVRDA
                     AARSRAEDALFDDLGIEADTGLDRCFHRRCSRWITRLLVNTPVTPSQVSIASLAVGSA
                     AIWSFWRATPLSAVWGVILYAIATILDHADGEIARLTYQESPSGANLDWTVDTIIHAG
                     LVLGMAVTAGGPLMQGVGLFGALGVTLSALFARHLPLGMTAGAGAGGMLKTLGSRDLV
                     YLLLLSFVAFRWLAPSLLPPLAVVVGIGSQAYWIACLARILKERTSR"
     gene            complement(2552..>2742)
                     /locus_tag="VNU03_07465"
     CDS             complement(2552..>2742)
                     /locus_tag="VNU03_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWP74336.1"
                     /translation="ASTLTEVLAPDRLRAAGVLDAEAVGRLVTEHVGGVRDHRRILWA
                     LLVFETWRAHHCGTPSPR"
BASE COUNT          461 a          868 c         1050 g          363 t
ORIGIN      
        1 tgtacaggtg gtagacatga cgggcgccaa cccgctctcc gggtagggcc aggggcagcc
       61 cggaaaggcc ggcgtggtac cggctggcca ggtggcggcg gtgatcgttt atggcgtcca
      121 ggcgacggag cagcacgcgg cccgcggcgg cctggatgtc gttgaaacgg aggttgaatc
      181 cgatctcgag gtggacgtcc ttgctgcgac gcccgtggtc gcgcagccgg cggcatcggg
      241 cggccacgtc gtcgtcgtcg gtgacgagga ggccgccgtc gcccatcacg gtgaggttct
      301 tggacgggta gaaggagaag gcaccggccc gcccgaagcc gcccaccttg cgtccctgcc
      361 aggtcgcgcc gtgcgcctgg gcgcaatcct cgagaagcca gaggccgcgc tccgcgcaga
      421 gctgcccgat gccgggcagg tcggccggat gcccgtagag gtgcaccggt acgaagccca
      481 cggtgcgcgg ggtgatcttg gcggcggcgt ccgtcagatc caccgtgtag gactcgtcga
      541 cgtccacgaa gatgggcacg ccgcccgcga agcaaacggc ctcgacggtc gggaatgcgg
      601 tatgcgaggg aacgaggacc tcgtcgcctg gcttgactcc gaaggccctg agcgccatcc
      661 agagggccgc ggtggcgctg ctgctcacca cggcgtgccg gctgccggtg tagcgggcga
      721 actcctcctc gaaggccgtg cattcgggac cgaggatgta ctggcgcgag tcgatggcgg
      781 cgaggaccgc ggccttgatc tcatcgtcga gcgggggacg ggaaagcgga atcggcatga
      841 actcgatcct cctggcgatg aatgccggtg tactcgttgg ccgccagtac ggtgcgttac
      901 gcctcggcgg cgatggcagc aggcatagag cgacacactg atgtgacccg gcgcgagccg
      961 ggactgcgac gggtcagccg ccgctaccgc gatgggggat gcttaggaga gcggcggggc
     1021 tcgagcgtct gcggtggagg agtacggcgt tgagcctgga ccctggggtt cgagggcgac
     1081 ggcgggcccg atcacgggaa acggcgtcca gcagcacaag gggaacgcat cgagcgtggc
     1141 cagacttgaa gtgatgagga agacaccatt gtcattgtcg tcgtcatcaa agaagccact
     1201 cacccaggtc gggtctggag gttcagcatg ggcgagtggt gtcagcgcca ggagcgcggt
     1261 cagcaatgcg gcgacgagcg cgagcgtcgg gagccggcgc gccaacgtca ccgactcgtg
     1321 cgttctttga ggatacgggc cagacatgcg atccagtagg cctgcgaacc gatcccgacg
     1381 accaccgcca gcggagggag caaggagggc gccagccacc ggaaggccac gaagctcagc
     1441 aacagcaggt acacgagatc gcgactgccg agggttttca gcatcccgcc cgcgcccgcg
     1501 ccggccgtca tcccgagcgg gagatgccgg gcgaacagcg cgctgagggt gacgccaagg
     1561 gcgccgaaga ggccgacccc ctgcatcagc ggtccccccg ccgtcaccgc catgcccagc
     1621 acgaggcccg cgtggatgat cgtgtcgacg gtccagtcga gattggcgcc ggacggcgat
     1681 tcctggtagg tgagccgcgc gatctccccg tctgcgtgat ccagaatggt cgcgatcgcg
     1741 tagaggatca cgccccagac tgcactgagc ggggtggcgc gccagaagga ccagatggcg
     1801 gcgctcccga cggccaggct ggcgatactg acttggctcg gcgtgaccgg ggtgttcacc
     1861 agcagccggg tgatccagcg agagcagcga cggtggaagc aacggtcgag ccccgtgtcg
     1921 gcctcgatac cgaggtcatc gaagagagcg tcctccgcgc gggaccgagc cgccgcgtcc
     1981 ctcaccgcaa cgaaggggtc ggtggacgcg cggggctggg cgccccattc cttgaggagc
     2041 cgagccagat ccgggcctac cggcgagccg gaggccagtc ggctccagag ggcgccgaca
     2101 atcgcccgcg gcaggatggc caccggggcc gggtcgagag gcgaggtggt catgatcact
     2161 cggtcggagg tcgcggctcc cgcgatcagg ctctgaaggg tgcgggtgtg caccaggctc
     2221 gcggcgggga cgagcagcca aggctgatcg ggtgggccgg ccggcagtcg gctgccgggc
     2281 tcgagccagc ggacggcggc ggccagggcc ggcatgtgtg cgagcgcccg ctcgacggcc
     2341 cggtcacgga gggcggcggg caccgcgatg atggaagcgc cggcgcgcag cgcggcgacc
     2401 agattccgga ccgtgacggt ccggccagcg atggagcgag ccgcgagccg cacggacgcg
     2461 gcatccggaa ggtagagaag ggccgcaccc gatcgacttg tgcgcaccgg gcgggtccga
     2521 accgggcgaa gcgtttccgg ggccggtggt ctcatcgcgg cgacggggtc ccgcagtggt
     2581 gggcccgcca ggtctcgaac acgagtagcg cccagaggat tcgccggtgg tctcgaacgc
     2641 caccgacgtg ttcggtcacc agtcgtccca ccgcctccgc gtcgagcacg ccggcggcgc
     2701 gcagtcgatc gggggcgagc acctcggtga gggtgctcgc ga
//
LOCUS       DATMCV010000320         2621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_419326, whole genome shotgun sequence.
ACCESSION   DATMCV010000320 DATMCV010000000
VERSION     DATMCV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419326"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="VNU03_07470"
     CDS             complement(<1..98)
                     /locus_tag="VNU03_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259446985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(54..56),aa:Sec)
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWP74337.1"
                     /translation="MTEPVTVTIFSDYVUPWCYLSTVRIEKLRTEHG"
     gene            complement(108..1073)
                     /locus_tag="VNU03_07475"
     CDS             complement(108..1073)
                     /locus_tag="VNU03_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HWP74338.1"
                     /translation="MTPVVLGAGPYRYEVHDAWAKLPPGREFNADVAAVGVDAQDRVY
                     AFNRGRHPMVVFDRDGNFLRSWGEGVFHRPHGVHVAPDDTLWLTDDGDHTVRHCTLDG
                     KVLLTLGIPGSPKPYMSGEPFHRCTHAALSPQGDLYVSDGYGNARVHKYDPSGRLLLS
                     WGEPGTDPGQFNIPHNICCDADGWVYVADRENHRIQVFNGNGRFETQWHDMHRPSGLH
                     LERAGEGRFYVGEIGGALPVNYDVPNIGPRIAIYSHRGERLARLGDRPAGLEPGQFVS
                     PHGLAVDSRGDIYVGEVSFTNWGNRYKGQPLPPGLRSLQKLVKAR"
     gene            complement(1070..2026)
                     /locus_tag="VNU03_07480"
     CDS             complement(1070..2026)
                     /locus_tag="VNU03_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HWP74339.1"
                     /translation="MTTTVETALGSIPTSELGATLMHEHIVTRSPGVQENWPHLWDRA
                     GILALAEKKMADLHARGIRAIVDLTTVDLGRDLDLIVQVARRSRVHVIVATGVWWMPQ
                     RYFSGHGVDEVAALFIRDIQQGIGATGVKAAIIKCATDTAGVTPVIENILRASARAQK
                     ATGVPISTHTWAAGRTGEAQQAIFAQEGVDLRRVIIGHSGDSDDLGYLRGLMERGSTI
                     GMDRFGLEHFLPTAKRVEVIARLCAEGYAPRMVLSHDANCWSDMLSEDDKRRTRPLWH
                     YNHIPDDILPALRKAGVSDDQIDQMLVRNPRAIFEARNGAAA"
     gene            complement(2052..>2621)
                     /locus_tag="VNU03_07485"
     CDS             complement(2052..>2621)
                     /locus_tag="VNU03_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWP74340.1"
                     /translation="RIGCHMLSATGRKYLRGPRGTGFLYVRRDTITALEPPFIDLEAA
                     AWIDADTYTVRDDARRFENWERFVAGQIGLGVAARYAQALGLDLIEARVKALAASLRG
                     ELAKRPGITVHDQGTERCGIVTFLRAGEAPAATRERLRALDINVTHTRASSSRLDLPA
                     RGLDALVRASVHYYNDEAEVERFLRAVAG"
BASE COUNT          359 a          872 c          980 g          410 t
ORIGIN      
        1 ccgtgctcgg tccggagctt ctcaatacgc acggtactga gatagcacca gggtcagacg
       61 tagtcggaga agatcgtcac cgtcaccggc tcggtcatcg tggctcctca gcgcgccttc
      121 acgagcttct gcaggctgcg cagcccgggt ggaaggggct gacccttgta gcgattgccc
      181 cagttggtga atgatacctc gcccacgtag atgtcgccgc gcgagtcgac cgcgaggccg
      241 tgcggggaga cgaactggcc cggctcgagg ccggccggcc ggtcgcccag gcgggcgaga
      301 cgctcgccac ggtggctata gatggcgatg cgcggcccga tgttgggcac gtcgtagttg
      361 accggcagcg ccccgccgat ctcgcccacg tagaagcgcc cctcgccggc gcgctcgaga
      421 tgaaggcccg acggccggtg catgtcgtgc cactgggtct cgaagcgccc gttgccgttg
      481 aagacctgga tgcggtggtt ctcccggtcg gccacgtaga cccagccgtc ggcgtcgcag
      541 cagatgttgt gcgggatgtt gaactggccc gggtcggtgc ccggctcgcc ccacgagagg
      601 agcaggcggc cgctcggatc gtacttgtgc acgcgcgcgt tgccgtagcc gtcggagacg
      661 tagaggtccc cctgcggcga gagcgcggcg tgagtgcagc ggtggaacgg ctcgccgctc
      721 atgtagggct tcgggctccc cggaatgccg agggtgagca gcaccttgcc gtcgagcgtg
      781 cagtgccgga cggtgtggtc gccgtcgtcg gtgagccaga gggtgtcgtc gggcgccacg
      841 tggacgccgt ggggccggtg gaacacgccc tcgccccagg agcgcaggaa gttgccgtcg
      901 cggtcgaaga ccaccatcgg atgccggccc cggttgaagg cgtagacgcg atcctgcgcg
      961 tcgacgccga ccgcggccac gtcggcgttg aactcgcggc ccggcggcag cttggcccac
     1021 gcgtcgtgga cctcgtagcg ataggggccg gcgcccagga cgaccggcgt cacgcggccg
     1081 ccccgttgcg ggcctcgaag atcgcccggg gattgcggac cagcatctgg tcgatctggt
     1141 cgtcggagac tcccgccttg cgcagggcgg gcaggatgtc gtccgggatg tggttgtagt
     1201 gccagagcgg gcgggtgcgg cgcttgtcgt cctcggagag catgtcgctc cagcagttgg
     1261 cgtcgtgcga gaggaccatg cggggcgcgt agccctcggc gcacagccgg gcgatgacct
     1321 cgacccgctt ggcggtgggc aggaagtgct cgagcccgaa gcggtccatc ccgatggtgc
     1381 tcccgcgctc catgagcccg cgcaggtagc cgaggtcgtc gctgtcgccg ctgtggccga
     1441 tgatcacgcg gcggaggtcg acgccctcct gggcgaagat cgcctgctgg gcctccccgg
     1501 tccgtcccgc ggcccaggtg tgggtcgaga tcgggactcc ggtcgccttc tgggcccgcg
     1561 cggaggcgcg caggatgttc tcgatgaccg gggtcacgcc ggcggtgtcg gtggcgcact
     1621 tgatgatggc ggccttgacc ccggtggcgc cgatcccctg ctgaatatcg cggatgaaga
     1681 gcgcggccac ctcgtcgacc ccgtggccgc tgaagtagcg ctgcggcatc caccagaccc
     1741 cggtggccac gatcacgtgc acgcgcgagc gccgggccac ctgcacgatc aggtcgaggt
     1801 cgcgcccgag atcgacggtg gtcaggtcca cgatcgcccg gatgccccgg gcgtggaggt
     1861 cggccatctt cttctcggcg agcgcgagga tgcccgcgcg atcccacagg tgcggccagt
     1921 tctcctgcac cccgggcgag cgggtcacga tgtgctcgtg catcagcgtg gccccgagct
     1981 cggaggttgg gatcgaaccg agcgctgtct ctaccgtcgt cgtcatggcg acctccagcc
     2041 ggctccgagc ttcatcccgc caccgcgcgc aggaagcgct cgacctcggc ctcgtcgttg
     2101 tagtagtgga cgctcgcgcg cacgagggcg tcgagcccgc gcgcgggcag atccaggcgt
     2161 gaggacgacg cccgggtgtg ggtgacgttg atgtcgagcg cccggagccg ctcgcgcgtc
     2221 gcggccggcg cctcgccggc ccggagaaag gtcacgatgc cgcatcgttc cgtgccctgg
     2281 tcgtgcaccg tgatgcccgg tcgcttcgcg agctcgccgc ggagcgaggc cgcgagcgcc
     2341 ttgacccgcg cttcgatcag gtcgaggccg agggcctggg cgtagcgggc cgcgaccccg
     2401 agcccgatct gtccggccac gaaccgctcc cagttctcga agcggcgcgc gtcgtcgcgt
     2461 acggtgtagg tgtcggcgtc gatccacgcg gcggcctcga gatcgatgaa gggcggctcg
     2521 agggcggtga tggtgtcgcg ccggacgtac aggaatccgg tgccgcgggg gccacggaga
     2581 tacttgcgcc cggtggccga gagcatgtgg cagccgatgc g
//
LOCUS       DATMCV010000321         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_882420, whole genome shotgun sequence.
ACCESSION   DATMCV010000321 DATMCV010000000
VERSION     DATMCV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882420"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VNU03_07490"
     CDS             complement(<1..823)
                     /locus_tag="VNU03_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWP74341.1"
                     /translation="MPFLSGKQAFLQILKQEGVDTMFGNPGTTELPLMDGLVREPSIR
                     YILALQEAVAISMADGYAQASGKLGVVNVHTSPGLGNAMGMLYDAHEAGTPLLITAGQ
                     HDQAMNLTEPILWSDLPPVARPYVKWSHEITRIEDLPRAVRRAAKTALAHPTGPVFIS
                     LPVDVLNAERELDLLQPTRVAPRIRGDRAAIEAAADLLVKAGRPILIAGDAVAHGDAL
                     AEMAELAETLGAPVYTECVPSTCSFPFTHPLYHGPFPRLGQPIRQLLMQHDLLFSV"
     gene            900..1937
                     /gene="hemE"
                     /locus_tag="VNU03_07495"
     CDS             900..1937
                     /gene="hemE"
                     /locus_tag="VNU03_07495"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWP74342.1"
                     /translation="MAPGASPPFLQACLRRPVPFTPIWLMRQAGRYMPEYRAVRSRLG
                     FLELCKNPEAAAEVTLQPVERLGVDAAILFADILLVLEPLGVGLEFTRGDGPRIGRPV
                     RSSQAARALPQIDVDDALGFVYETVARVRKALADRVPLIGFAGAPFTLASYLIEGGPS
                     REFLLTKAFMRQEPEAWAAMMTRLADVTADYLNGQIGAGVQAVQLFDSWVGALSPADY
                     RAYVLPHSRRVMQALRPGVPVIHFGVGTGGLLPLMREAGGDVIGLDWRVELGSTWARL
                     GHDVAVQGNLDPAVLLANVDAIRVGAREILDAAAGRPGHVFNLGHGIHKETPVDHVRA
                     LVDIVHELSAR"
     gene            1934..>2586
                     /locus_tag="VNU03_07500"
     CDS             1934..>2586
                     /locus_tag="VNU03_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWP74343.1"
                     /translation="MSAVDSVLLVAFGGPERPEDIRPFLQIVTSGRAIPAARLEEVAH
                     HYEIIGGGSPLNALTRRQGDALATAPAVRDRALPVWVGMRNWHPFLHETLAEMKDRGR
                     RRAVGIILSSLQTEASWARYVDDVAAAQEKVGPDAPEVVFAPPWSDHPRFVDAMADRT
                     RAALEAIEPARRAAARLVFTAHSVPVAMAAGSPYAAQLEAAGRAIAGRLGRARFTIA"
BASE COUNT          326 a          911 c          970 g          379 t
ORIGIN      
        1 ccaccgagaa cagcaggtcg tgctgcatga ggagctggcg gatcggctgg cccaggcgcg
       61 ggaaggggcc gtggtagagc ggatgcgtga acgggaacga gcaggtggac ggcacgcact
      121 cggtgtagac cggggcgccc agcgtctcgg ccagctccgc catctcggcc agcgcgtcgc
      181 cgtgggcgac cgcgtcgccc gcgatgagga tgggccggcc cgccttcacg agcagatcgg
      241 cggccgcctc gatcgcggcg cgatcgccgc ggatgcgcgg ggcgacccgg gtcggctgca
      301 gcaggtcgag ctcgcgctcc gcgttcagca cgtcgaccgg cagcgagatg aacaccgggc
      361 cggtcggatg ggcgagcgcg gtcttggccg cgcggcgcac cgcgcggggc aggtcctcga
      421 tgcgggtgat ctcgtgcgac cacttcacgt agggccgcgc caccggcggc aggtccgacc
      481 agagaatggg ctcggtgagg ttcatcgcct ggtcgtgctg gcccgcggtg atgagcagcg
      541 gcgtgccggc ctcgtgggcg tcgtagagca tgcccatcgc gttgccgagg cccggcgagg
      601 tgtggacgtt caccaccccg agcttcccgc tcgcctgcgc gtagccgtcc gccatgctga
      661 tcgccaccgc ctcctgcagg gccaggatgt agcggatgct cggctcgcgc acgagcccgt
      721 ccatcagcgg cagctcggtg gtgcccgggt tgccgaacat ggtgtcgacg ccttcctgct
      781 tcaggatctg gagaaacgcc tgcttgccgg agagaaaggg catggggaag cctcctggcg
      841 cgggatgata gccccgcttc cttgactgcg ccagaccccg cctgtaggct cggaccagca
      901 tggcgccggg cgcgtcacct cccttcctgc aggcgtgtct ccgccggccc gtcccgttca
      961 ccccgatctg gctgatgcgg caggccggcc gctacatgcc ggagtaccgg gcggtgcggt
     1021 cgcggctcgg cttcctcgag ctgtgcaaga acccggaggc ggccgcggag gtgacgctgc
     1081 agccggtgga gcgcctgggc gtcgacgcgg cgatcctctt cgccgacatc ctgctcgtgc
     1141 tggagcccct cggggtcgga ctcgagttca cgcggggcga cggtccgcgc atcggccgcc
     1201 cggtccgtag tagccaagcg gcgcgtgctc ttccgcaaat agacgtcgac gacgcgctcg
     1261 gcttcgtcta cgagacggtg gcgcgcgtgc gcaaggccct cgccgatcgc gtgccgctga
     1321 tcggcttcgc gggggcgccc ttcacgctcg cgtcctacct gatcgagggc ggcccctcgc
     1381 gcgagttcct cctgaccaag gccttcatgc ggcaggagcc ggaggcgtgg gccgccatga
     1441 tgacgcgact cgccgacgtc accgccgact atctgaacgg ccagatcggc gccggagtgc
     1501 aggcggtgca gctcttcgac tcgtgggtgg gcgcgctgtc gccggccgac tatcgggcct
     1561 acgtgctgcc ccacagccga cgggtcatgc aggccctgcg gccgggcgtt ccggtcatcc
     1621 acttcggcgt cggtaccggc ggcctgctgc cgctgatgcg agaggcgggc ggcgacgtga
     1681 tcgggctcga ctggcgggtg gagctcgggt ccacctgggc gcggctcggc cacgacgtgg
     1741 cggtgcaggg gaatctcgac cccgcggtgc tgctggcgaa cgtcgacgcg atccgggtcg
     1801 gggcgcgcga gatcctcgac gcggcggcgg gtcgcccggg gcacgtcttc aacctcgggc
     1861 acggcatcca caaggagacg ccggtcgacc acgtgcgggc gctggtcgac atcgtccacg
     1921 agctgtccgc gcgatgagcg cggtggactc ggtgctgctc gtcgccttcg gcgggccgga
     1981 gcggcccgag gacatccgcc cgttcctcca gatcgtcacg agcgggcgcg cgatccccgc
     2041 cgcgcgcctg gaggaggtcg cgcaccacta cgagatcatc ggcgggggct cgcccctcaa
     2101 tgccctcacg cgccggcagg gcgacgcgct cgccaccgcg cccgcggtcc gggatcgcgc
     2161 gctgccggtc tgggtcggca tgcggaactg gcaccccttt ctccacgaga ccctcgctga
     2221 gatgaaggac cggggtcggc gccgcgcggt cggcatcatc ctctcgtcgc tgcagaccga
     2281 ggcctcgtgg gcccgctacg tggacgacgt ggccgccgcg caggagaagg tcggccccga
     2341 cgcgcccgag gtcgtcttcg cgccgccgtg gtctgatcac ccgcgcttcg ttgacgcgat
     2401 ggcggaccgc acccgcgcgg cgctggaggc gatcgagccg gcgcgccggg ccgccgcgcg
     2461 cctcgtgttc accgcgcata gcgtgccggt ggccatggcc gcgggctcgc cgtatgcggc
     2521 gcagctggag gcggcggggc gggcgatcgc ggggcggctc ggccgcgcgc gcttcacgat
     2581 cgccta
//
LOCUS       DATMCV010000322         8261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_549812, whole genome shotgun sequence.
ACCESSION   DATMCV010000322 DATMCV010000000
VERSION     DATMCV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549812"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /gene="lhgO"
                     /locus_tag="VNU03_07505"
     CDS             complement(<1..700)
                     /gene="lhgO"
                     /locus_tag="VNU03_07505"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HWP74344.1"
                     /translation="MIQSADLAVVGGGIVGLATALALTEAHPRLRLLLLEKEPQIAAH
                     QTGHNSGVIHSGIYYKPGSLKARTCVEGARRMKQFCDTHGIKWEGCGKVIVATDAAEL
                     PRLQSIYERGQANGLSGLKMLSAEEVRELEPNCRAVRAIHVPETGIVDYVQVAGKMAE
                     LIQQRGGRILTGAGVSAIRRLGSGLVLETAQGPVEARHLVNCGGLHSDRVAGLTGVTP
                     EVRIIPFRGEYYMIR"
     gene            complement(771..1532)
                     /locus_tag="VNU03_07510"
     CDS             complement(771..1532)
                     /locus_tag="VNU03_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWP74345.1"
                     /translation="MKLVSWNVNGLRSAWKKGFPDFLAAQAADVVCVQETKLQAEQVT
                     DEMRSPGGYASHWSFAERPGYSGVVTYSRAVPLAASTVCDHPVLDCEGRLVHVELPDF
                     HLLNVYFPNGGMGPERVRHKLAFYEAFLAFAESLWKRGKGVVVCGDVNTAHTELDLAR
                     PRENEATSGFLPAERAWVARFIAAGYHDTFRLFVADPGHYTWWDLKTGARARNVGWRI
                     DYFFVSDELRGRIAGARILPGILGSDHCPIVLELA"
     gene            complement(1529..2287)
                     /locus_tag="VNU03_07515"
     CDS             complement(1529..2287)
                     /locus_tag="VNU03_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP74346.1"
                     /translation="MRRVLVTGMSGLIGTAVRRRLEGRFALRALNRRPVAGVECHQAD
                     IGDLAAIVPAFDGVDAVVHLAANVEANPPVDAIVRDNVVGTYNVFEAARRAGVRRVIY
                     ASSGATVSDWERDEPYAALVAGRYDQVAEWPILTHESPTRPHGLYGCSKIWGEALARH
                     FADTTPLSMICLRIGRVTAADRPVGPRELSVWCSQRDIAQMVERCLVAPDNLRFDVFF
                     VCSENKWTYRDIEHARAMVGFVPEDRAEDHRGRS"
     gene            2383..3156
                     /locus_tag="VNU03_07520"
     CDS             2383..3156
                     /locus_tag="VNU03_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP74347.1"
                     /translation="MLDLGLTGKVAIVTGGSEGLGRATAMRFAQEGCHVVICARRKDV
                     LERAAEVIRKQTGVQVLAQTADVTRPADVEAVVNATLAAHGGVDILVNNAGTSSAGPF
                     EKVDDATWVADIEVKLMAAVRFCRLVIPQMKKRGGGRIVNVTTVGGKVPVARSLPTSV
                     TRAAGINLTKSLANEYAPDHILVNTVCLGLVKSAQWERRAKGGDMEAYYREAAKRVPV
                     GRVGEAEEFADLVAFLCSARAAYITGTAVNFDGGMSAAF"
     gene            3261..4712
                     /locus_tag="VNU03_07525"
     CDS             3261..4712
                     /locus_tag="VNU03_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HWP74348.1"
                     /translation="MGPLAATPVGAKVLSALRWSAATRLVSQAVSWAMTVFVIRLLSP
                     SDYGVLAMAVILPSALYLLNDLGLDVVLVQRLAPGEEFRRQVFGVVIVVNLLCALILI
                     TTAPLVAAFFSEPILVPILRVLSLQFLLLVFETLPRARLEQGLDFRSQSLISLGANWL
                     GGLTTLALALTGWGVWALVWGRLVSTAATTLALNVAAPSLCLPSFSLAMVRRALSFGG
                     IVTLERGAWQFFSDADKVIGGRLWSDATLGLYSVAQDLATMPMHRTGSLISSIGLPAF
                     SQVQGRLDDLRFYLLKATRITSVLSFPIFIGLAVTAPEAVAVLLGAKWPAAAPLLQIL
                     ALIMPLRMVSTLLPPALWSIGRPGISAGNVLIAALIIPSACLIGAQWGAVGMAMAWLG
                     AYPLVFWITLQRSGGVLGLGVPDFGRAMRAPALASLAMAGVVIGTRYLLPDHAGAVAR
                     LLVLVPAGALTYLAALLGLERGALRETAQLVGR"
     gene            complement(4740..5480)
                     /locus_tag="VNU03_07530"
     CDS             complement(4740..5480)
                     /locus_tag="VNU03_07530"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HWP74349.1"
                     /translation="MLGGLLLGGLLGGLFFGSGGGGIGMIEILIIGGLAWFAISYMRR
                     QQAAAPSGYATSGGYGPVPASRYEPSSAGGTATPAGTATPAGGTATMEAPAGPADLER
                     GLTHIRQMDPGFDPRAFSETASDVFFKVQGAWTARSMAGVSSLLMPEMSGTLQRDCDR
                     LKAEHKVNRLENIAVRSAEVTEAWQESGQDFVTVHFLASLLDYTTDESGAQVLEGSRT
                     EPVKFEEYWTFTRPVGPAAFRLSAIQQA"
     gene            5820..6863
                     /locus_tag="VNU03_07535"
     CDS             5820..6863
                     /locus_tag="VNU03_07535"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HWP74350.1"
                     /translation="MNALRRSFLKLLGAAGAAAVTPGPALADRIRILPDGAGPVDPGL
                     PGQVSPDLQRTLGQLTVGPAVTQAGLSVLWLVGGDAKGPAALEVLTLDEARARGALTV
                     VERAQASVPDLIAENRGKAHVLLLAGEILIGGKQNRVLREDLLLPPLSGPRNVSVYCV
                     EQGRWNEGRKDFESRSSVVQPSVRSEVLRKAEQGRIWSGVAAASRNLQAASPTGSYQA
                     VYDTPEVKAHLDRATRSLETVPLPVVVGAAAFVGPSLAGVDAFHAGGLFAREWPKLLR
                     AYALDGYRAAPSGWDEAAARSRVDGLLRGAARVEGTLRGSVGVGRLFEFALDKQRGAA
                     LLFEGQAIHVAIL"
     gene            6970..7512
                     /locus_tag="VNU03_07540"
     CDS             6970..7512
                     /locus_tag="VNU03_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP74351.1"
                     /translation="MAARLPYLDRAQVPPEIQSLYDTLQAGSGRVLNIFRLMAHHARS
                     LPPFHAWYPRLREGPLDLRLRYLAYVRASQLNRCRYCVTHNGAAGRRAGIPKEQLEAL
                     ADHRTSPLFSELERLVITYAEAMTTEVQVDGALVETLRQRLGPEALVQLTLTVAAANF
                     TNRFNEALGTELEREEARDA"
     gene            7505..7918
                     /locus_tag="VNU03_07545"
     CDS             7505..7918
                     /locus_tag="VNU03_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74352.1"
                     /translation="MPDTVQRVDYFHVQAPNKPGEGARYLAALQEAGINLVAFSGFPE
                     GRGSQLDFVPADAAAFRGFARRSKWKITGPKRAFLLGGEDRVGVICEMLGKLAAARIN
                     VTAVDAVCAGAGRYGAILWVAARDYNRAATLLGAI"
     gene            complement(7962..>8261)
                     /locus_tag="VNU03_07550"
     CDS             complement(7962..>8261)
                     /locus_tag="VNU03_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74353.1"
                     /translation="LAVGLARDLARIALEGRPVATAPDRHPGELRLCLESTLGLGAVA
                     AGLAWRLGAASAARPVSVGGLVLALAAVATLGHLTRNLVVVLPREPAHRNVVFWS"
BASE COUNT         1109 a         3007 c         2971 g         1174 t
ORIGIN      
        1 cccggatcat gtagtactcg ccgcggaacg ggatgatgcg cacctcgggc gtcaccccgg
       61 tgagcccggc gacccggtcg gagtggagcc cgccgcagtt gacgaggtgg cgcgcctcga
      121 ccgggccctg cgcggtctcg agcacgaggc cgctccccag ccgccggatc gcgctcaccc
      181 cggcgccggt caggatgcga ccgccgcgct gctggatcag ctccgccatc ttgccggcca
      241 cctggacgta gtccacgatg ccggtctcgg gcacgtggat cgcccgcacc gcgcgacagt
      301 tcggctcgag ctcgcgcacc tcctcggccg agagcatctt gaggccggag agcccgttgg
      361 cctggccgcg ctcgtagatg ctctgcagcc gcggcagctc ggccgcgtcg gtggccacga
      421 tgaccttgcc gcagccctcc cacttgatgc cgtgggtgtc gcagaactgc ttcatccggc
      481 gcgcgccctc cacgcaggtc cgcgccttga gcgagccggg cttgtagtag atgccggagt
      541 ggatgacgcc gctgttgtgg ccggtctggt gagccgcgat ctgcggctcc ttctccagca
      601 ggagcaggcg gaggcgcgga tgggcctcgg tcagggccag cgcggtggcg agcccgacga
      661 tgccgccccc gacgaccgcg agatcggcgc tctggatcac ggccgagaga ataccaccac
      721 cctctcccct ccggggagag ggcagggtga ggggcccagg gtgaggggta ctaggccagc
      781 tcgagcacga tcgggcagtg atccgacccc aggatcccgg gcaggatgcg cgcgcccgcg
      841 atccggccgc gcagctcgtc ggagacgaag aagtagtcga tgcgccagcc cacgttccgc
      901 gcccgcgccc ccgtcttcag atcccaccag gtgtagtgcc ccgggtccgc gacgaagagg
      961 cggaaggtgt cgtggtagcc cgccgcgatg aagcgggcca cccacgcgcg ctcggcgggc
     1021 aggaaccccg acgtcgcctc gttctcgcgc gggcgggcga gatcgagctc ggtgtgcgcg
     1081 gtgttcacgt cgccgcacac cacgaccccc ttcccccgct tccagagcga ttccgcgaac
     1141 gcgaggaacg cctcgtagaa ggcgagcttg tgccggacgc gctccggacc cattcccccg
     1201 ttcgggaagt agacgttgag cagatggaag tcgggcagct cgacgtgcac gaggcggcct
     1261 tcacagtcca gcaccggatg gtcgcagacg gtgctcgccg cgagcgggac cgcgcgggag
     1321 taggtgacca ccccgctgta gcccgggcgc tccgcgaagg accaatggct cgcgtagcct
     1381 ccgggggacc gcatctcgtc ggtcacctgc tccgcctgca gcttggtctc ctgcacgcac
     1441 acgacgtcgg cggcctgggc ggccaggaaa tcggggaagc ctttcttcca cgccgagcgg
     1501 agcccgttga cgttccagga gacgagcttc acgagcggcc gcggtggtcc tcggcgcggt
     1561 cctcgggcac gaagcccacc atcgcccgcg cgtgctcgat gtcgcggtac gtccacttgt
     1621 tctccgagca cacgaagaac acgtcgaatc gcaggttgtc cggcgcgacc aggcagcgct
     1681 cgaccatctg ggcgatgtcg cgctggctgc accataccga gagctcgcgc ggcccgaccg
     1741 gccggtccgc cgcggtcacc cgaccgatgc gcaggcagat catcgagagc ggcgtggtgt
     1801 ccgcgaagtg gcgggcgagg gcctcgcccc agatcttgct gcagccgtag agtccgtggg
     1861 gccgcgtggg cgactcgtgg gtgaggatcg gccactccgc cacctggtcg tagcggcccg
     1921 cgaccagcgc cgcgtagggc tcgtcccgct cccagtcgct cacggtggcc ccgctgctgg
     1981 cgtagatcac gcggcgcacc cccgctcggc gggccgcctc gaagacgttg taggtgccta
     2041 ccacgttgtc ccgcacgatc gcgtcgaccg gcgggttcgc ctccacgttc gcggccagat
     2101 gcaccaccgc gtccactccg tcgaacgcgg ggacgatcgc cgcgagatcg ccgatgtcgg
     2161 cctggtggca ctccacgccc gcgaccggcc ggcgattcag cgcgcgcagg gcgaagcgcc
     2221 cctcgagccg acggcgcacc gcggtcccga tgagcccgct catcccggtg accaggaccc
     2281 gacgcatggc gctgaccata tcacgggggg tgccgggggt gcgcgccgcg ccttgaccgc
     2341 ggtcgaggcc gcggctagta tccgcgaccg ggaggaccca ccatgctcga tctcggtctc
     2401 accggcaagg tcgccatcgt caccggaggc agcgaggggc tcgggcgcgc caccgcgatg
     2461 cgattcgccc aggagggctg tcacgtggtg atctgcgcgc gccgcaagga cgtgctggaa
     2521 cgcgcggcgg aggtcatccg gaagcagacc ggcgtgcagg tgctggccca gaccgccgac
     2581 gtcacccggc ccgccgacgt ggaggcggtg gtgaatgcga cgctggccgc gcacggcggc
     2641 gtggacatcc tcgtcaacaa cgcgggcacc tcgtccgcgg ggccgttcga gaaggtggac
     2701 gacgccacat gggtggccga catcgaggtc aagctcatgg ccgcggtgcg cttctgtcgc
     2761 ctcgtcatcc cccagatgaa gaagcgcggc ggcggccgga tcgtcaacgt gaccacggtg
     2821 ggcggcaagg tcccggtggc ccgctcgctg cccaccagcg tgacccgcgc ggccggcatc
     2881 aacctcacca agtcgctcgc caacgagtac gcgcccgacc acatcctggt gaacacggtc
     2941 tgcctggggc tggtgaagag cgcccagtgg gagcgccggg ccaagggcgg cgacatggag
     3001 gcctactacc gcgaggccgc caagcgggtg ccggtgggcc gggtgggcga ggccgaggag
     3061 ttcgccgacc tggtggcctt cctctgctcg gctcgcgccg cgtatatcac cggcaccgca
     3121 gtcaatttcg acgggggcat gtccgccgcc ttctagcgcc ccgggccggc ctcccgggcc
     3181 gggagccgcc ggcctcgggg ggccgtgttc gttgtccgaa aagcccccgc ccggttagtc
     3241 tgcccggtta gtctggatgg gtgggtcccc tcgccgccac cccggttgga gccaaggtcc
     3301 tgtccgcgct gcgctggtcg gccgccaccc ggctggtcag ccaggcggtg tcctgggcga
     3361 tgaccgtctt cgtcatccgc ctgctctcgc cgtccgacta cggcgtgctg gcgatggcgg
     3421 tgatcctccc ctccgcgctc tacctcctga acgatctggg cctggacgtg gtcctcgtcc
     3481 agcggctggc cccgggcgag gagttccgcc gccaggtgtt cggcgtggtg atcgtggtga
     3541 acctgctctg tgccctgatc ctgatcacca ccgcgccgct cgtggccgcg ttcttcagcg
     3601 agccgatcct ggtcccgatc ctgcgcgtgc tgtcgctgca gttcctgctg ctcgtcttcg
     3661 agacgctgcc gcgcgcccgc ctcgagcagg ggctcgactt ccgcagccag tccctgatca
     3721 gcctcggcgc gaactggctc ggcggcctca ccacgctcgc gctcgccttg acgggctggg
     3781 gcgtctgggc gctggtgtgg gggcgcctgg tgtcgaccgc cgccaccacc ctcgccctca
     3841 acgtggccgc gccgtcgctc tgcctgccga gcttctcgct cgcgatggtg cggcgcgcgc
     3901 tctccttcgg cggcatcgtc acgctcgagc gcggcgcctg gcagttcttc agcgacgccg
     3961 acaaggtcat cgggggccgg ctctggtccg acgccaccct cggcctctac tcggtggcgc
     4021 aggacctcgc gacgatgccg atgcaccgga ccggcagcct gatctcctcg atcggcctgc
     4081 ccgcgttctc ccaggtgcag ggtcgcctgg acgacttgcg gttctatctg ctcaaggcca
     4141 cccgcatcac cagcgtgctg tcgttcccga tcttcatcgg gctcgcggtc accgcgccgg
     4201 aggcggtcgc ggtcctcctc ggcgccaagt ggccggccgc ggccccgctc ctgcagatcc
     4261 tcgccctgat catgccgctg cgcatggtgt cgaccctgct gccccccgcc ctctggagca
     4321 tcggccgccc gggcatcagc gcgggcaatg tgctgatcgc cgcgctcatc atcccgtcgg
     4381 cctgcctcat cggcgcccag tggggcgcgg tgggcatggc gatggcatgg ctcggggcct
     4441 acccgctggt gttctggatc acgctgcagc gctcgggcgg ggtgctgggg cttggggtgc
     4501 cggacttcgg acgggcgatg cgggccccgg ccctggcgag cctggcgatg gccggcgtgg
     4561 tgatcgggac gcgatatctc ctgcccgatc acgccggcgc cgtcgcgcgc ctcctggtgc
     4621 tggtgccggc gggcgcgctc acctacctgg ccgcgctcct cggtctcgag cgcggcgcgc
     4681 tccgcgagac ggcccagctc gtcgggcgct gacccggcga gccgcggccg ccgccgcggt
     4741 caggcctgct ggatcgcgct gaggcggaac gcggcgggtc ccaccggccg cgtgaacgtc
     4801 cagtactcct cgaacttcac cggctcggtc cggctgccct cgagcacctg cgcgccgctc
     4861 tcgtcggtgg tgtagtcgag caggctcgcc aggaagtgca cggtgacgaa gtcctggccg
     4921 ctctcctgcc aggcttcggt gacctccgca gagcggaccg cgatgttctc gagccgattc
     4981 accttgtgct cggccttcag ccgatcgcag tcgcgctgga gcgtccccga catctcgggc
     5041 atgagcagcg acgacacccc cgccatgctg cgcgccgtcc aggcgccctg caccttgaag
     5101 aagacatccg aggcggtctc cgagaacgcg cgaggatcga agcccggatc catctggcgg
     5161 atgtgggtca ggccacgctc gagatcggcc gggccggccg gcgcctccat cgtggcggtg
     5221 ccgccggccg gcgtggcggt gccggccggc gtggcggtgc cgcccgcgga cgacggctcg
     5281 tagcgcgagg ccgggacggg tccgtagccg ccggatgtcg cgtagccgga gggcgcggcc
     5341 gcctgctggc gccgcatgta ggagatcgcg aaccaagcga gcccaccgat gatcaggatc
     5401 tcgatcatcc cgatgccgcc cccgccggaa ccgaagaaca gcccgccgag caggccgccc
     5461 agcaggaggc ctccgagcat cccgcccatc atgccgcccc agcccgagcg cgcaggcgca
     5521 ggctgctgaa ggcccggcga cgtcggctgg ctcggggcag cggggctcga ggacggcggc
     5581 cgggagggtg acgagtagct ccgcgagccg cggctgccgc tcgagctgcc cccgcccgcc
     5641 cgggcccagg cctcggtgac ataaagaacc ggagagagcg ccagtgccac caggatgatc
     5701 gttgccaagg tccgcttcat agcgtccctc ctcgagacct gtcggtgcgt cgccgtcagt
     5761 atacgcgatg cggggaactt tcggcgccgg agcaggtcta agcgggcacg gaggacgata
     5821 tgaatgcatt gcgacgctcg tttctgaagc tcctgggcgc ggccggcgct gccgcggtca
     5881 cgccggggcc agccctggcc gaccgcatcc gcatcctgcc cgacggcgcc ggccccgtcg
     5941 atcccgggct gcccggccag gtcagccccg acctccagcg caccctcggg cagctcacgg
     6001 tcggcccggc ggtcacccag gcggggctgt cggtgctctg gctggtcggg ggggacgcga
     6061 aaggccccgc cgccctcgag gtcctgacgc tcgacgaggc ccgcgctcgc ggcgctctca
     6121 cggtggtgga gcgcgcgcag gccagcgtgc ccgacctgat cgcggagaac cgcggcaagg
     6181 ctcacgtcct gctgctggcc ggcgagatcc tgatcggtgg caagcagaac cgggtgctgc
     6241 gcgaggatct gctgctcccg ccgctcagcg gaccccggaa cgtctcggtg tactgcgtgg
     6301 agcagggccg ctggaacgag ggtcgcaagg acttcgagag ccgatcgagc gtggtgcagc
     6361 cgagcgtgcg ctcggaggtg ctgcgcaagg cggagcaggg ccgcatctgg tcgggggtcg
     6421 ccgccgcgtc gcggaacctg caggcggcct ccccgaccgg gagctatcag gcggtctacg
     6481 acacgcccga ggtgaaggcg catctcgacc gggcgactcg cagcctggag acggtcccgc
     6541 tgcccgtggt ggtcggcgcc gcggccttcg tgggcccgag cctggccgga gtggacgcgt
     6601 tccacgccgg cgggctcttc gcccgagaat ggcccaagct gttgcgcgcc tacgcgctcg
     6661 acggctaccg ggccgccccg agcggctggg acgaagcggc ggcgcgaagc cgcgtggacg
     6721 gcctgctgcg cggagccgcg cgcgtcgagg gcaccctgcg cgggagcgtg ggggtcggac
     6781 ggctcttcga gttcgccctc gacaagcagc gcggcgccgc gctcctcttc gaggggcagg
     6841 cgatccacgt ggcgatcctg tgagcggcgg cttgaccgcc cggaggggcc gcgcaatgat
     6901 gcggccacgc gccgagccgc gcgcggggcc gagcccgccg ctttccccgg cgcggaggag
     6961 acggccccga tggcggcgag acttccctac ctggaccggg cgcaggtacc gcccgagatc
     7021 cagtcgctct acgacacgct ccaggcgggt agcgggcgcg tgctcaacat cttccggctc
     7081 atggcccacc acgcgcggtc cctgccgccg ttccacgcct ggtacccgcg cctgcgcgag
     7141 gggccgctcg atctgcggct acgctacctc gcctacgtgc gggcctcgca gctcaatcgc
     7201 tgtcgctact gcgtgactca caacggggcg gcgggtcgcc gggcggggat cccgaaggag
     7261 cagctcgagg cgctggccga ccatcgcacg agcccgctct tctccgagct cgagcggctg
     7321 gtgatcacct acgcggaggc catgaccacc gaggtccagg tggacggcgc cctcgtggag
     7381 acgctccgtc agcgtctggg gccggaggcg ctcgtccagc tcaccctcac cgtggccgcg
     7441 gccaacttca ccaaccgctt caacgaggcg ctcggcaccg agctcgagcg cgaggaggcc
     7501 cgcgatgcct gacaccgtgc agcgcgtcga ctacttccac gtgcaggcgc cgaacaagcc
     7561 gggggaaggc gcgcgctacc tcgccgcgct ccaggaggcc ggcatcaacc tggtcgcgtt
     7621 ctccggcttc ccggagggac ggggctccca gctcgacttc gtgcccgccg acgccgccgc
     7681 cttccggggc ttcgcccggc gctcgaagtg gaagatcacc gggcccaagc gcgccttcct
     7741 gctcggcggc gaagaccgcg tgggggtgat ctgcgagatg ctcggcaagc tcgcggcggc
     7801 gcgcatcaac gtcaccgcgg tcgacgcggt gtgcgcgggt gcgggccgct acggggccat
     7861 cctgtgggtg gccgcgcgcg actacaaccg ggccgccacg ctgctgggcg cgatctgagg
     7921 ccacgccacg cgggaggtga cagcgggcgc gcgacgcgcc ctcaggacca gaagacgacg
     7981 ttgcggtggg ccggctcccg ggggaggaca acgaccagat tccgcgtgag gtgaccgagc
     8041 gtcgccaccg cggccagcgc gagcacgagg ccgccgaccg acaccggccg cgccgcgctc
     8101 gcggccccga ggcgccaggc gagacccgcc gcgaccgcgc cgaggccgag agtggactcg
     8161 aggcagaggc gcagctcacc ggggtggcga tccggggcgg tggcgaccgg gcggccctcg
     8221 agcgcgatgc gcgcgaggtc gcgagcgagc ccgaccgcga g
//
LOCUS       DATMCV010000323         2314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_216105, whole genome shotgun sequence.
ACCESSION   DATMCV010000323 DATMCV010000000
VERSION     DATMCV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216105"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="VNU03_07555"
     CDS             <1..1560
                     /locus_tag="VNU03_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP74354.1"
                     /translation="VDVALLLERGPAGGSGDLVVEDLSRAFGGVRAVSGVGFTARRGH
                     ITSVIGPNGAGKTTLINLISGVIRADAGRARLGARDLSGLPAHAVARAGVTRTYQTSQ
                     LFAGMSVLDNVLVGLQRGRLGSPVAPRRAGTSPDIAVAASLLDFVGYAGSLDAPAGAL
                     PHVDRRLVEIARALAPRPAALLLDEPAAGLSAEDTRRVGALLRAIAARGVAVLLVEHD
                     MELVMAVSTHVVVLDAGLVLAEGTPATIQADPAVRAAYLGDRRSAGRARAAAAPASRD
                     TALNVAGLVAGHGGAPVLPGLDLSVGAGELVSVLGANGAGKSTLMGALSGLLRAEGGG
                     VQLFGADVSALDAPDRVRAGLVLVPEGRLVFPDLSVRDNLRLGALLRRDADIGADVEA
                     MLDRFPRLRDRADGRAGLLSGGEQQMLALARGLMARPRVLLLDEPSLGLAPQLVEQVF
                     EELATLRDAGRTLLLVDQNAELSLAIADRGYVLERGRVVAEGPAATLLGDGALLDAYL
                     GGARPKEAPTS"
     gene            1557..>2314
                     /locus_tag="VNU03_07560"
     CDS             1557..>2314
                     /locus_tag="VNU03_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HWP74355.1"
                     /translation="MTLMHVPVIDIAPYRTGDRAAARAVASQVGEACRDIGFLVIAGH
                     GIDPALVQRVDDTARAFFDLPLADKMRVARPAPDVTRGYVPIEGEAVAASRGEVTPGD
                     LNESMMIGPVDVPETDPYFRAPEAGKHFHPNLWPERPTDLRPAWTAYFRAMGDLAVVV
                     MRIFATALDLREDFFDGKIDRHISRLRLRNYPEQPEPPRPGQLRAGAHADYGSLTILK
                     AEANPGGLEVCNQAGDWVAVPIVPGTFVVNLGEL"
BASE COUNT          257 a          925 c          826 g          306 t
ORIGIN      
        1 gtcgacgtcg ccctcctcct cgagcggggg cccgccggcg ggagcggcga tctcgtcgtc
       61 gaggatctct cgcgcgcgtt cggcggcgtc cgcgcggtgt ccggcgtcgg cttcaccgcg
      121 cggcgcggcc acatcacgag cgtcatcggg ccgaacggcg cgggcaagac cacgctcatc
      181 aacctgatca gcggcgtgat ccgggcggac gcgggccgcg cgcgcctcgg cgcccgcgat
      241 ctctccggtc tgcccgccca cgcggtggcc cgcgccggcg tgacgcgcac ctatcagacc
      301 tcccagctct tcgccggcat gtcggtcctc gacaacgtgc tggtcgggct ccagcgcggg
      361 cggctcggct ccccggtggc gccccggcgc gccggcacct cgccggacat cgcggtcgcc
      421 gcctcgctcc tcgatttcgt cggctacgcc gggagcctcg acgccccggc cggcgccctc
      481 ccccacgtgg accggcgcct ggtcgagatc gcgcgcgccc tcgccccgcg gccggccgcg
      541 ctcctgctcg acgagcccgc cgccgggctc agcgccgagg acacgcgccg cgtgggagcc
      601 ctgctgcggg ccatcgccgc gcgcggcgtg gcggtcctgc tggtcgagca cgacatggag
      661 ctggtgatgg cggtctccac ccacgtcgtg gtgctcgacg ccggtctcgt cctggccgaa
      721 ggcacgccgg cgacgatcca ggccgacccc gcggtgcgcg ccgcctacct gggcgatcgc
      781 cgctcggcgg gccgcgcccg ggcggccgcg gcgcccgctt cgcgcgacac ggccctgaac
      841 gtggccggcc tcgtcgccgg ccacggcggc gcccccgttc tgcccggcct cgacctgtcg
      901 gtgggcgcgg gcgagctcgt ctcggtcctc ggcgccaacg gggccggcaa atcgaccctg
      961 atgggggcgc tctccgggct cctccgcgcc gagggcggcg gcgtgcagct cttcggcgcc
     1021 gacgtgagcg cgctcgacgc gccggaccgc gtgcgcgccg gcctcgtgct ggtgcccgag
     1081 ggccgcctgg tcttccccga cctgagcgtg cgcgacaacc tgcggctcgg cgcgctgctc
     1141 cggcgcgacg ccgacatcgg cgccgacgtc gaggcgatgc tggatcgctt cccgcgcctg
     1201 cgcgatcggg ccgacgggcg cgcggggctc ctctcgggcg gcgagcagca gatgctggcg
     1261 ctcgcccggg gcctgatggc gcgtccgcgc gtcctcctcc tcgacgagcc gtcgctcggc
     1321 ctggccccgc agctcgtcga gcaggtcttc gaggagctgg ccaccctccg cgacgccggg
     1381 cgaacgctcc tgctcgtcga ccagaacgcc gagctgagcc tcgccatcgc cgaccggggc
     1441 tacgtgctcg agcggggacg cgtggtcgcc gagggcccgg cggccacgct cctcggcgac
     1501 ggggccttgc tcgacgccta cctcggcggg gcccgtccga aggaggcgcc cacgtcatga
     1561 ccctcatgca cgtgcccgtc atcgacatcg cgccctaccg caccggcgac cgcgccgccg
     1621 cccgcgcggt ggccagccag gtgggcgagg cgtgccgcga catcggcttc ctcgtcatcg
     1681 ccggtcacgg catcgacccc gccctcgtcc agcgggtcga cgacaccgcc cgcgccttct
     1741 tcgatctgcc cctggccgac aagatgcgcg tggcgcggcc cgcgcccgac gtgacccgcg
     1801 gctacgtccc gatcgagggc gaggcggtgg cggcgagccg gggcgaggtg accccgggcg
     1861 acctcaacga gtcgatgatg atcggcccgg tcgacgtgcc ggagaccgac ccgtacttcc
     1921 gcgctcccga ggccggcaag cacttccatc caaatctctg gcccgagcgg ccaacggacc
     1981 tgcgaccggc gtggaccgcc tacttccgcg cgatgggcga cctggccgtc gtcgtcatgc
     2041 gcatcttcgc gacggccctc gacctgcgcg aggacttctt cgacggcaag atcgatcggc
     2101 acatcagccg cctgcgcctg cgcaattacc ccgagcagcc ggagcccccg cgaccgggcc
     2161 agctccgcgc cggcgcgcac gcggactacg gaagcctcac catcctgaag gcggaggcca
     2221 accccggcgg gctcgaggtg tgcaaccagg ccggggactg ggtcgcggtg ccgatcgtgc
     2281 ccggcacgtt cgtggtcaac ctcggcgagt tgat
//
LOCUS       DATMCV010000324         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_679698, whole genome shotgun sequence.
ACCESSION   DATMCV010000324 DATMCV010000000
VERSION     DATMCV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679698"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VNU03_07565"
     CDS             complement(<1..110)
                     /locus_tag="VNU03_07565"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWP74356.1"
                     /translation="MIKVYVSSVIEAPADAVWAQIRDFNGLPKWTPFVADS"
     gene            complement(115..1833)
                     /locus_tag="VNU03_07570"
     CDS             complement(115..1833)
                     /locus_tag="VNU03_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HWP74357.1"
                     /translation="MTGAQFGSAILGLVVAAIVVAIVVWLMNWLYLRSSKERAFVRTG
                     LGGQKVVMNGGAFVLPIVHEVVPVNMNTLRLEVSRGRDKALITKDRMRVDVVSEFYVR
                     VQAGPDAIASAAQTLGQRTLEPETLKELVEGKFVDALRTVAAEMTMEELHEKRGEYVK
                     RVKGVVAADLLQNGLELEAVSLTQLDQTAMEFFNPSNAFDAEGLTRLTEQIERRKKIR
                     NDIEQDTMIQVRNKNLETERQALDIDRELEYARLAQERELEMQRASQRADVARQRADK
                     EQEAERAQIGAREAVDRARISSERAVEEERIGKEREVEQLEIARRQAVELAEQQRAIA
                     VAEQSKAQSEAQGAADLARARAVAEEERVFTVRETEMAERRKRIDLIGAQQEAERDAL
                     RVRVGAEAEKAAAGDRGAAVRAEAEAYADSERVRAMAARIRNEVEAEGTRLMNEAQNL
                     LSPEARLSAMRIRLIDKLEGIIRESVKPMERIEGIKILQVDGLGGGGGGRGDGRDGGS
                     GGGFADSVVNSALRFRAQAPLVDQLLREIGVDSGDVGKAARGLLDRSQSAALPQPGGL
                     DQTPAE"
     gene            complement(1862..>2395)
                     /locus_tag="VNU03_07575"
     CDS             complement(1862..>2395)
                     /locus_tag="VNU03_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HWP74358.1"
                     /translation="FMITDDFLRRLIALGFSGVQNFPTVGLFDGTIRAQLEETGMGYG
                     LEVEMIRAAHERDLLTTPYVFSEQNAVDMAKAGADIVVCHLGLTAGGAIGAETALKLR
                     DCVPLVDAWAAAARSVNKDVIVLCHGGPIARPADARFILKHCRQCHGFYGASSMERLP
                     TEVALTETTRKFKKIRR"
BASE COUNT          308 a          910 c          782 g          395 t
ORIGIN      
        1 gagtcggcca cgaaaggcgt ccacttgggc agaccgttga agtcgcggat ctgcgcccac
       61 accgcgtccg cgggcgcctc gatcacgctc gacacgtaga ccttgatcac ggggctattc
      121 ggcgggagtc tggtcgagcc cgccgggctg cggcagcgcc gcgctctggc tgcgatcgag
      181 caggccgcgg gccgccttgc cgacgtcgcc cgagtccacc ccgatctcgc gcaggagctg
      241 gtccaccagc ggcgcctgcg cccggaaccg gagcgccgag ttcaccacgc tgtccgcgaa
      301 gcctcccccg ctgccgccgt cgcgcccgtc gccgcggccg cccccacccc cgccgagacc
      361 atcgacctgt aggatcttga tgccctcgat acgttccatc ggcttgaccg actcgcggat
      421 gatgccctcg agcttgtcga tcaaccggat gcgcatcgcg gacaggcgcg cctccgggga
      481 gagcaggttc tgggcctcgt tcatcaggcg agtcccctcc gcctccacct cgttgcggat
      541 gcgcgcggcc atcgcccgca cgcgctccga atcggcgtac gcctcggcct ccgcgcgcac
      601 cgcggcgccc cggtctcccg cggccgcctt ctcggcctcc gcgccgacgc gcacccggag
      661 ggcgtcgcgc tccgcctcct gctgggcccc gatcaggtcg atgcgcttgc gccgctcggc
      721 catctcggtc tcgcgcacgg tgaagacgcg ctcctcctcg gccaccgccc gggcccgggc
      781 cagatcggcc gcgccctggg cctccgattg cgccttcgat tgctcggcca ccgcgatggc
      841 ccgctgctgc tcggccagct cgacggcctg gcgccgcgcg atctcgagct gctctacctc
      901 gcgctccttg ccgatccgct cctcctcgac cgcccgctcc gaggagatgc gcgcgcggtc
      961 gaccgcctcc cgcgccccga tctgggcccg ctcggcctcc tgctccttgt cggcgcgctg
     1021 gcgggccacg tcggcccgct gggacgcgcg ctgcatctcg agctcgcgct cctgggccag
     1081 ccgcgcgtac tccagctcgc ggtcgatgtc gagcgcctgg cgctcggtct cgagattctt
     1141 gttgcgcacc tggatcatgg tgtcctgctc gatgtcgttg cggatcttct tgcgccgctc
     1201 gatctgctcg gtgagccggg tgaggccctc cgcgtcgaag gcgttggacg ggttgaagaa
     1261 ctccatcgcg gtctggtcga gctgggtgag cgagaccgcc tccagctcga gtccgttctg
     1321 caggaggtcg gccgccacca cccccttcac ccgcttgacg tactcgcccc gcttctcgtg
     1381 cagctcctcc atggtcatct cggccgccac ggtgcgcagc gcgtccacga acttgccctc
     1441 gaccagctcc ttcagcgtct ccggctcgag cgtgcgctgg cccagggtct gggccgcgga
     1501 ggcgatcgcg tcggggcccg cctgcacacg cacgtagaac tcggagacca cgtcgacccg
     1561 catgcggtcc ttggtgatca gcgccttgtc ccgcccgcgg gagacctcga ggcgcagcgt
     1621 gttcatgttg accggcacca cctcgtggac gatcggcagc acgaaggccc cgccgttcat
     1681 caccaccttc tggcccccga ggccggtacg cacgaaggcg cgctccttcg aggagcgcag
     1741 gtacagccag ttcatcagcc acaccacgat cgcgaccacg atggccgcga ccacgagccc
     1801 gaggatcgcc gatccgaact gtgctccagt catgactgtc tcctaggttg ctgcatatct
     1861 ctcaccggcg gatcttcttg aacttgcggg tcgtctcggt cagcgccacc tcggtcggca
     1921 gccgctccat gctggaggcg ccgtagaagc cgtggcactg ccggcaatgc ttcaggatga
     1981 agcgcgcgtc ggcgggccgg gcgatcggtc cgccgtggca gagcacgatg acgtccttgt
     2041 tcaccgagcg ggcggcggcc gcccacgcgt cgacgagcgg cacgcagtcc cggagcttga
     2101 gcgcggtctc ggcgccgatg gcgccgccgg cggtgagccc gaggtggcag accacgatgt
     2161 cggcccccgc cttggccatg tccaccgcgt tctgctccga gaacacgtag ggggtggtga
     2221 gcaggtcgcg ctcgtgggcc gcgcggatca tctcgacctc gaggccgtag cccatcccgg
     2281 tctcctccag ctgggcgcgg atggtcccgt cgaagaggcc gacggtgggg aagttctgca
     2341 ccccggagaa gccgagcgcg atcagccgcc ggaggaagtc gtccgtgatc atgaa
//
LOCUS       DATMCV010000325         4174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_350332, whole genome shotgun sequence.
ACCESSION   DATMCV010000325 DATMCV010000000
VERSION     DATMCV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350332"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VNU03_07580"
     CDS             <1..547
                     /locus_tag="VNU03_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP74359.1"
                     /translation="VGLGNIGSAFARRVAALETHVIACDPYVDDARFKSLGVERVDLA
                     TLAARSDYVSVHTLLNDETRGLINEAFLRRMKPTACLINTSRGPVVDEAALAPALEQG
                     WLAGAALDVFEEEPLGAESPLRRLDNVILTPHAAYFSSPAVAQVPRRCGEEVARVLTK
                     ERPLHVVNPEVYAPGHRRRGP"
     gene            562..1398
                     /locus_tag="VNU03_07585"
     CDS             562..1398
                     /locus_tag="VNU03_07585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP74360.1"
                     /translation="MHLGIFSYNVDYGARPDELARAAEERGFESFWVGEHTHIPASRV
                     TPYPGGEPLPKPYYHMADPFVSLMAAAAATRTIKLGTGICLVVEHDPIVLAKSVATLD
                     WLSNGRVLFGIGGGWNREEMEDHGTPFDRRWQVLRERVLAMKALWTQEEASFHGEFVN
                     FDRAISFPKPVQRPHPPILFGGATAKGRARVVEYCDGWIPIDVLLDDLPAAIVDLRHK
                     AETAGRGLDTLSVSVFAFQPPKADAVARLRDMGIERVTMVAPRRLADALPFLDRMASL
                     NR"
     gene            1401..2696
                     /locus_tag="VNU03_07590"
     CDS             1401..2696
                     /locus_tag="VNU03_07590"
                     /inference="COORDINATES: protein motif:HMM:NF027805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HWP74361.1"
                     /translation="MHTLAFLDPAHFHAALTLGDRHPQIRDDLVVHAPKGPELGDFLA
                     LVDAFNQRPERPTAWRVDVRPGEGSLERLVAERPGDVVVLAGRNDRKLATIRRLHDAG
                     FHVLADKPWMTGPDGLDDLRHVLAAGPLAVEMMTGRHEITAILAARLAGDEPVFGRFA
                     DGGADEAGIEITSVHHLEKTVNGRPLRRPPWYFDVRVQGDGIADIPTHMVDQAQRLVR
                     AARPGPAGDALALELLGARTSSTPVPRALFARVTGMAEFPPELASVVSSAELAYRGNA
                     QLSFRAGPVRVALDTRWELSAPPGGGDAHRSVIRGTRSVIRVEQGPSTGFRRRLSVEP
                     RGEPGPVQAALERALAKWSGAHPGLSLAPAAGGWEIRVPPALDTGHERQFPVVLAEFL
                     TLVEEGRRPARLAADTLAKYTLLAQASTEARQRDGAAPP"
     gene            2693..3505
                     /locus_tag="VNU03_07595"
     CDS             2693..3505
                     /locus_tag="VNU03_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP74362.1"
                     /translation="MTAESDRIAQYWGREHLAEAILAALTARGVSLDALTVDVLAPVD
                     QFHGGGKPVTGRLARLAAIRPGTRVLDVGGGFGGPARTLAVEFGCHVTGIDLTDSYVR
                     AAKVLTARMGLDDRVTHQVGDALALPFPDGAFDLVWTQNTGMNIADKERLYAGFHRVL
                     RPGGLLALQEPMAGPVQPVVYPVMWARDPSTSFLRTPEAMRAVIEAAGFRARVWEDMT
                     TETSGPVTGAEVPAHSIQRLVMGDAIDAIIAAGHRNRSEGRIVSIQAVFERP"
     gene            3549..>4174
                     /locus_tag="VNU03_07600"
     CDS             3549..>4174
                     /locus_tag="VNU03_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP74363.1"
                     /translation="MSDALTGQTVELLQQMIRNQCVNDGTVQSGQEVRTSDLLRTYLG
                     GSDVEVYEPVAATGRTSLVARIEGSDPEAPTLCLMGHTDVVPVNPKTWTRDPFGGELV
                     NGEVWGRGALDMLNITASQAVAFKTLARRGWRPRGTLVYLACADEEAGGTLGAGHVCA
                     QHWDALDADYCLTENGGTVKAGPGGHGLNVTVHVGEKGVAWRRLRVKGT"
BASE COUNT          558 a         1590 c         1477 g          549 t
ORIGIN      
        1 cgtgggcctg ggcaacatcg gcagcgcctt cgcccgccgc gtcgcagcgc tcgagacgca
       61 cgtcatcgcg tgcgatccgt acgtcgacga cgcgcgcttc aagtcgctcg gggtcgagcg
      121 cgtcgatctg gccaccctgg ccgcccggtc ggactacgtc tcggtccaca cgctcctgaa
      181 cgacgagacg cgcgggctga tcaacgaggc cttcctccgg cgcatgaagc ccaccgcgtg
      241 cctgatcaac acctctcgcg gcccggtcgt cgacgaggcg gcgctcgcgc ccgcgctcga
      301 gcagggctgg ctcgccggcg ccgcgctcga tgtcttcgag gaggaaccgc tcggcgccga
      361 gagcccgctg cgccgcctcg acaacgtcat cctcaccccg cacgccgcct acttctcgtc
      421 gcccgcggtg gctcaggtgc cgcgccgctg cggcgaggag gtcgcccggg tcctcaccaa
      481 ggagcggccg ctgcacgtgg tcaaccccga ggtctacgcg cccggccatc ggcggcgcgg
      541 gccctgagcg ggcggcgcgc gatgcacctc ggcatcttca gctacaacgt ggactacggc
      601 gcgcggcccg acgagctggc gcgcgccgcg gaggagcggg gcttcgagtc cttctgggtg
      661 ggcgagcaca cccacatccc ggccagccgc gtcactccgt atccgggcgg cgagcccctg
      721 cccaagccgt actaccacat ggccgatccg ttcgtctcgc tcatggcggc cgccgcggcc
      781 acccgcacca tcaagctcgg caccggcatc tgcctggtgg tggagcacga ccccatcgtg
      841 ctggccaaga gcgtggccac gctcgactgg ctctcgaacg ggcgcgtgct cttcggcatc
      901 ggcggcggct ggaaccgcga ggagatggag gaccacggca ccccgttcga ccggcgctgg
      961 caggtgctcc gcgagcgggt gctggccatg aaggccctct ggacccagga ggaggcgtcg
     1021 ttccacggcg agttcgtcaa cttcgaccgc gcgatctcgt tcccgaagcc ggtgcagcga
     1081 ccgcatccgc cgattctctt cggaggcgcc accgccaagg gccgcgcccg cgtcgtcgag
     1141 tactgcgacg ggtggatccc catcgacgtc ctgctcgacg accttcccgc cgccatcgtc
     1201 gatctgcgcc acaaggcgga gaccgccggc cgcgggctcg acacgctctc cgtctccgtc
     1261 ttcgccttcc agccgccgaa ggccgacgcg gtcgcgcgtc tgcgggacat ggggatcgag
     1321 cgcgtcacca tggtggcgcc gcggcgcctg gccgacgccc tgcccttcct cgaccggatg
     1381 gcgagcctca accggtagcc gtgcacaccc tggcgttcct cgatccggcc cacttccacg
     1441 ccgcgctcac gctcggcgac cgacacccgc agatccggga cgatctggtg gtgcacgccc
     1501 cgaaaggccc cgagctgggc gatttcctcg ccctggtcga cgcgttcaac cagcgccccg
     1561 agcgccccac cgcctggcgt gtggacgtcc gccccggcga ggggtcgctc gagcgcctgg
     1621 tggccgagag gcccggcgac gtggtggtgc tcgccggccg gaacgaccgc aagctggcga
     1681 cgatccgccg gctgcacgac gccggctttc acgtcctggc cgacaagccc tggatgaccg
     1741 gcccggacgg gctcgacgat ctccgccacg tgctcgcggc cggaccgctc gcggtcgaga
     1801 tgatgaccgg gcggcacgag atcaccgcga tcctcgccgc ccggctggcg ggagacgagc
     1861 cggtgttcgg ccgcttcgcg gacggcggag ccgacgaggc gggcatcgag atcacgagcg
     1921 tccatcacct ggagaagacg gtcaacggcc gccccctccg gcgcccgccc tggtacttcg
     1981 acgtccgcgt ccagggcgac gggatcgccg acatcccgac ccacatggtg gatcaggccc
     2041 agcgcctcgt gcgcgccgcc cgcccgggcc cggccggcga tgcgctcgcc ctcgagctgc
     2101 tcggcgcgcg cacgtcgagc acgccggtgc cgcgggcgct cttcgcgcgg gtgaccggca
     2161 tggccgagtt cccgcccgag ctcgcctccg tggtcagcag cgcggagctg gcctatcgcg
     2221 gcaacgcgca gctctcgttc cgcgccgggc cggtccgggt ggccctcgac acgcgctggg
     2281 agctatccgc tccgcccggc gggggcgacg cccaccggtc ggtgatccgc ggcacgcgca
     2341 gcgtgatccg cgtcgagcag gggcccagca ccgggttccg gcgtcgcctc tcggtcgagc
     2401 cgcgtggcga gccgggtccc gtgcaggccg cgctcgagcg cgcgctggcc aagtggtcgg
     2461 gcgcgcatcc cggcctctcc ctcgcgcccg cggccggcgg gtgggagatt cgcgtgccgc
     2521 ccgcgctcga cacgggccac gagcgccagt tcccggtcgt gctggccgag ttcctgaccc
     2581 tggtcgagga gggccgccga cccgcccgcc tcgccgcgga caccctcgcc aagtacaccc
     2641 tgctcgcgca ggcttccacc gaggcccggc agcgcgatgg agccgcaccg ccatgaccgc
     2701 cgagtcggac cgcatcgcgc agtactgggg gcgcgagcac ctggccgagg cgatcctcgc
     2761 cgcgctgact gcgcgcgggg tgagcctcga cgccctcacg gtagacgttc tcgccccggt
     2821 cgaccagttc cacggcggag gcaagccggt caccggccgc ctcgcgcgcc tggccgcgat
     2881 ccggcccggg acccgcgtcc tcgacgtggg cggcggcttc ggcggccccg cccgcacgct
     2941 cgcggtcgag ttcggctgcc acgtcaccgg gatcgacctg actgactcct acgtgcgcgc
     3001 cgcgaaggta ttgaccgccc ggatgggcct cgacgaccgg gtcacccacc aggtcggcga
     3061 cgcgctcgcc ctgcccttcc ccgacggcgc gttcgacctg gtgtggacac agaacaccgg
     3121 catgaacatc gccgacaagg agcgcctgta cgcgggcttc catcgcgtcc tgcgtccggg
     3181 cgggctgctc gccctgcagg agccgatggc ggggccggtg cagccggtcg tctacccggt
     3241 catgtgggcg cgcgacccat ccacgagctt cctgcgcacg ccggaggcga tgcgcgcggt
     3301 gatcgaggcc gccggcttcc gggcgcgcgt gtgggaggac atgaccaccg agacctccgg
     3361 gcccgtcacc ggggcggagg tgcccgccca cagcatccag cgcctggtga tgggcgacgc
     3421 gatcgacgcg atcatcgcgg cgggccaccg caaccgcagc gagggccgca tcgtgtcgat
     3481 ccaggcggtc ttcgagcgcc cgtgaccgcc ctcgacaacg cgcggcccgc tggctaggat
     3541 accgacccat gagcgacgcg ctgaccggcc agaccgtgga gctgctgcag cagatgatcc
     3601 gcaaccagtg cgtcaacgac ggcaccgtgc agtccggcca ggaggtccgc accagcgatc
     3661 tcctgcgcac ctacctcggg ggcagcgacg tcgaggtcta cgagccggtg gcggccacgg
     3721 gccgcacgag cctggtcgcc cgcatcgagg gcagcgatcc ggaggcgccc acgctgtgcc
     3781 tcatgggcca caccgacgtg gtgccggtga accccaagac ctggacgcgc gatcccttcg
     3841 ggggcgagct ggtgaacggc gaggtctggg ggcgcggcgc gctcgacatg ctgaacatca
     3901 ccgcctccca ggcggtggcc ttcaagaccc tggcgcgccg cggatggcgg ccgcggggca
     3961 cgctggtcta cctggcctgc gcggacgagg aggccggggg caccctcggg gccgggcacg
     4021 tgtgcgcgca gcactgggac gcgctcgacg cggactactg tctgaccgag aacggcggca
     4081 cggtcaaggc gggcccgggc ggccacgggc tcaacgtcac ggtgcacgtg ggcgagaagg
     4141 gcgtggcctg gcggcggctc cgtgtgaagg gcac
//
LOCUS       DATMCV010000326         3008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_882618, whole genome shotgun sequence.
ACCESSION   DATMCV010000326 DATMCV010000000
VERSION     DATMCV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882618"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VNU03_07605"
     CDS             complement(<1..658)
                     /locus_tag="VNU03_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWP74364.1"
                     /translation="MTHSTGSNADWIRRDDEAILGWRHFPRVVFERAQGLRLWDVEGR
                     EYLDFVAGHISLLLGHNHPELREVLIEQSAKLWHHYKYFAATPVVEFAETLKAALPPE
                     LDVINFAAVGSEANEVALRVARGVTKAFDVVAVIGGLYGGTLTVEGLNSIGGPRKRHL
                     GPLLSPARTSTILTPDCYRCPLGLKYPSCDIECVKASEEMTERVSTGDVAAIITETIL
                     G"
     gene            complement(695..1624)
                     /locus_tag="VNU03_07610"
     CDS             complement(695..1624)
                     /locus_tag="VNU03_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74365.1"
                     /translation="MSHVDVSAPAGPAAVPAAFAAETAPRPRRHRLTFKPAFGMVVGA
                     ALLLATLFPGQIAPSSPTDQRLAERLRPPAWMDGGTTGHLLGTDSLGRDVCSRVIYGA
                     RTTFLVALVSSLIGGIGGTGLGLLAGYHGGMLDELVRKLVDMQLAFPYLLLAISVMAV
                     AGRSLPILVVVLALSSWPAFARIVRGEVLSLRERQFIEGARAIGCDDRRILLAHVLPG
                     VTASLCVVWSFDLARVVVLESSLSFLGLGVQPPTPSWGMDLSESRQFLQIAYWTVLFP
                     GLAISLVVLAANLVGDWVRDVLDPIVKRNRAAA"
     gene            complement(1621..2550)
                     /locus_tag="VNU03_07615"
     CDS             complement(1621..2550)
                     /locus_tag="VNU03_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74366.1"
                     /translation="MGWLGEAGRALGHLLVVMLGTASLVFVVLRLTGDPAMMVAPPGA
                     TLDDLAAIRRELGLDHSIGVQYVEFLRRLAVGDLADSFRYRRPALEIVSERAMATVQL
                     ALVAQLLAIAVAIPLGVIAAIRRDSWLDGTLTGLATLGQSIPHFWLGLMAIVVFAVHL
                     GWLPTSGRGGWLHLVLPAITLSSYSMASLTRLMRSSVLEVLGSDYIRTARAKGLGAGL
                     VLFKHALKNAMIPVVTLTGLQLGVLLSGAVIVETVFAWPGVGRLIIEAIHTRDYPLVQ
                     AGVVLMALLFVVVNQGVDLCYRWLDPRMAGRRG"
     gene            complement(2563..>3008)
                     /locus_tag="VNU03_07620"
     CDS             complement(2563..>3008)
                     /locus_tag="VNU03_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74367.1"
                     /translation="DVATVNATTEMLRKVGINARAEIVDIQTRLEMIKNGNVTGLLLI
                     NPQGTNFDTDGVVWRLMGPGGILAVHWKGSDPDQEFARLMEEARYSVDPKKRQQNYYR
                     AAEIFADELPWIPVFQDVATFGVSAKVKNFKPRPDWLVLPQRLGL"
BASE COUNT          507 a         1041 c         1056 g          404 t
ORIGIN      
        1 ccccgaggat ggtctcggtg atgatcgcgg ccacgtcgcc ggtgctcacc cgctccgtca
       61 tctcctcgct cgccttcacg cactcgatgt cgcacgacgg gtacttgagg cccagcggac
      121 accggtagca atccggggtc aggatcgtcg aggtccgggc cgggctcagg agcgggccaa
      181 gatggcgctt gcgcggaccc ccgatgctgt tgagcccctc cacggtcagg gtgcccccgt
      241 agaggccccc gatcaccgcc accacgtcga acgccttggt gacgccacgg gccacccgca
      301 gcgcgacctc gttggcctcc gatcccactg ccgcgaagtt gatcacgtcg agctcggggg
      361 gcagggcggc cttgagggtc tccgcgaact cgaccaccgg ggtcgccgcg aagtacttgt
      421 agtggtgcca gagcttggcg gattgctcga tcaggacctc acgcagctcg gggtggttgt
      481 gcccgagcag cagcgagatg tggccggcca cgaagtcgag gtactcccgg ccctccacgt
      541 cccagaggcg caggccctgg gcgcgctcga agacgactcg cgggaagtgg cgccagccaa
      601 gaatggcctc gtcgtcacgc cgaatccagt ccgcgttgga acccgtcgaa tgtgtcatgt
      661 tcccgtctcg ctcccttggt gtcggagccg cccgctacgc cgcggcgcgg ttccgcttga
      721 cgatggggtc gaggacgtct cgcacccagt cgccgacgag gttggcagcc agaacgacca
      781 gactgatcgc caggccggga aacagcacgg tccagtaggc gatctgcagg aattgccgcg
      841 attcactcag atccatgccc cagctcgggg tgggcggctg gacgccgagc ccgaggaagc
      901 tcaggctcga ctccagcacg acgacccgcg cgagatcgaa gctccacacc acgcagagcg
      961 aggcggtcac cccgggcagc acgtgggcga gcaggatgcg ccggtcatcg cacccgatgg
     1021 cccgcgcccc ctcgatgaac tgacgctcgc ggagggacag cacctcgccg cgcacgatgc
     1081 gggcgaaggc cggccagctg ctgagcgcga ggaccacgac caggatgggc aagctgcgcc
     1141 cggcgacggc catgaccgag atggccagga ggaggtaggg gaaggccagc tgcatgtcga
     1201 ccagcttccg cacgagctcg tcgagcatgc caccgtggta gccggccagg agccccaggc
     1261 cggttccgcc gatgcccccg atcagggacg agacgagcgc caccaggaag gtcgtgcgcg
     1321 ccccgtagat cacccggctg cacacgtcgc gcccgaggga gtcggtgccg agcaggtggc
     1381 ccgtggtgcc gccgtccatc cacgcgggcg ggcgcagccg ctcggcgagc cgctggtcgg
     1441 tggggctgga cggagcgatc tggccgggaa agagcgtggc gagcagcagc gccgccccga
     1501 cgaccatgcc gaaggcgggc ttgaacgtca ggcgatggcg ccgcggccgc ggcgcggtct
     1561 ccgccgcgaa cgccgccggg acggccgcgg ggccggcggg ggccgagacg tccacgtggc
     1621 tcatccgcgc cgcccggcca tgcggggatc gagccagcga tagcagagat cgaccccctg
     1681 gttcaccacc acgaagagca gcgccatgag cacgacgccc gcctggacga gcgggtagtc
     1741 gcgggtgtgg atggcctcga tgatgagccg gccgacgccc ggccacgcga acaccgtctc
     1801 gacgatgacc gccccggaca gcaagacgcc gagctggaga ccggtgaggg tgacgaccgg
     1861 gatcatcgcg ttcttcagcg cgtgcttgaa gagcaccagc cccgccccga gccccttggc
     1921 ccgggcggtg cgaatgtagt ccgacccgag cacctcgagc acgctcgagc gcatcagccg
     1981 cgtgaggctc gccatcgaat aggacgagag cgtgatcgcg ggcagcacca ggtgcagcca
     2041 cccgccgcgg ccggaggtgg ggagccagcc gaggtgaacc gcgaacacca cgatcgccat
     2101 caggcccagc cagaagtggg gaatcgactg ccccagggtc gcgagcccgg tcagcgtccc
     2161 gtccagccac gagtcccggc gaatggccgc gatcacgccc agggggatcg cgaccgcgat
     2221 ggcgaggagc tgggcgacca gggcgagctg gacggtggcc atcgcccgct cgctcacgat
     2281 ctcgagcgcg ggccggcggt agcggaacga gtcggccaga tcccccaccg cgagccttcg
     2341 aaggaattcg acgtactgca ctccgatgga gtgatcgagg ccgagctcgc ggcggatcgc
     2401 ggccaggtcg tcgagggtcg ctcccggtgg cgcgaccatc atcgcgggat cgccggtgag
     2461 ccggagcacc acgaacacga ggctcgcggt gcccagcatc acgacgagga gatggcctag
     2521 cgcccgtccc gcctcgccaa gccatcccat cgggtaactc cgctacagcc ccaggcgctg
     2581 cggcagcacc agccagtcgg ggcgcggctt gaagttcttg accttggccg agaccccgaa
     2641 ggtcgccacg tcctggaaga ccgggatcca cggcagctcg tcggcgaaga tctcggcggc
     2701 ccggtagtag ttctgctggc gcttcttcgg gtccaccgag taccgcgcct cctccatgag
     2761 gcgggcgaac tcctggtccg ggtcactccc cttccagtgc accgcgagga taccgccggg
     2821 ccccatgagc cgccagacca ctccgtcggt gtcgaagttc gtcccctgcg gattgatcag
     2881 gaggagcccg gtgacgttgc cgttcttgat catctcgaga cgggtctgga tgtcgacgat
     2941 ctcggcgcgg gcgttgatgc cgaccttgcg gagcatctcg gtggtggcat tgacggtggc
     3001 cacgtcgt
//
LOCUS       DATMCV010000327         3135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_84851, whole genome shotgun sequence.
ACCESSION   DATMCV010000327 DATMCV010000000
VERSION     DATMCV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84851"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VNU03_07625"
     CDS             complement(<1..191)
                     /locus_tag="VNU03_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74368.1"
                     /translation="MSRQWVDDPGLQAEKEVALGFDLQRLEPEFLEDPYPTYHRLRRW
                     DPVHRCPDGSYFLTRYDDVA"
     gene            complement(204..869)
                     /locus_tag="VNU03_07630"
     CDS             complement(204..869)
                     /locus_tag="VNU03_07630"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWP74369.1"
                     /translation="MSRFIDLSVTVDENTFSPPSTNMKLEVTPHRRGPGFWQVSSVNQ
                     SLHTGAHIDSPLHVFKNGITTAEITLDQVMGEALVVDLSFVGANHEITIEDLKKGGAG
                     DVKRGDIVLLKTGWTDQMYGRWPDYFTRSPYLPPGAAQWLVERGPKNIGFDFFEEYCA
                     RLPDFTSEDFAMHRVILGAGVVIMEGLTNLGALPRRRVDFAAPFYKIAGTEGAPARFF
                     ATV"
     gene            complement(877..2310)
                     /locus_tag="VNU03_07635"
     CDS             complement(877..2310)
                     /locus_tag="VNU03_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74370.1"
                     /translation="MSTRSGWSAALMLAGLLACAPAAPAEEQRNMRLVGMDDLQARSA
                     YQPVVHHQGARWIAYLGHHGGKRMNPLTGQEELNGTSILDVTDPKHPRYLHHIPGAEG
                     EAEAGGAAMVRVCDGKTLPRAQKEKTYLLRTLGNLAHEVWDVTDPAKPARLVTVLDGL
                     KSTHKNWWECDTGIAYLVSDGAPSGWRTNRMTKIYDLGDPAQPRFIRDFGLPGQEPGS
                     TGVAPEGVHGPIALRNRVYFAYGTGAKGVLQIVDREKLLAGNPQAADRFAPTAENLLY
                     PQIGRLDMSPAWGGHTSFPILGVSVPHWSPGVPGRTRDFVLLVSESLRNECQEIRQLS
                     FMVDVTTERTPYSVATFEVPDPTGDFCRRGGRFGPHSSNESFSPIYYGRLAFIAYFNA
                     GVRAVDVRDPFHPTEAGFYIPSVTPKTDKRCVEVAGGPPRCKTAIQTNNVDVDDRGFI
                     YLADRADTGLHIVELTGPARAIAYFTK"
     gene            2488..>3135
                     /locus_tag="VNU03_07640"
     CDS             2488..>3135
                     /locus_tag="VNU03_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74371.1"
                     /translation="MAEPRGTARVTFLVNAAGNFLGAAVAFLYFRLVDHHAASLPRVS
                     LHEVVHPLLVFAGLLALGNWLNNRWVAPINRATQGHPLSEREAALVRRRALLFPYVLA
                     GVSFAGWVVAGLIWGFGMPLMMGRVVSLSDSVRIVFGITVLAGTVTTAFIFFVSERIW
                     RTRLLLLFPEGDLSAVPRVPRLAVRVRLLAIFLLTAVVPLAVLGIVAYARGLDLLD"
BASE COUNT          433 a         1067 c         1103 g          532 t
ORIGIN      
        1 gccacgtcgt cgtagcgcgt caggaaatag gagccgtccg ggcagcgatg taccgggtcc
       61 catcgccgca gtcggtgata ggtcgggtac gggtcctcga gaaattcggg ctccaggcgc
      121 tgcaggtcga agccgagcgc aacctccttc tctgcctgga gccctggatc atccacccac
      181 tgccggctca tcacgcgggc cgcttacacc gtcgcgaaga agcgcgcggg ggcgccctcg
      241 gtgcccgcga tcttgtagaa gggcgcggca aagtctacgc gacggcgggg cagcgcgccc
      301 aggttggtca ggccttccat gatgaccacg cccgcgccca ggatcacccg gtgcatcgcg
      361 aagtcctccg aggtgaagtc gggcagccgc gcgcagtact cctcgaagaa gtcgaagccg
      421 atgttcttgg gcccgcgctc caccagccac tgggcggccc cgggcggcag gtacggcgac
      481 cgggtgaagt agtcgggcca gcggccgtac atctggtcgg tccagccggt cttgagcagc
      541 acgatgtcgc cgcgcttgac gtcgcccgcc ccgcccttct tgaggtcctc gatggtgatc
      601 tcgtggttgg cccccacgaa ggacagatcc accacgagtg cctcgcccat gacctggtcc
      661 agcgtgatct cggcggtggt gatgccgttc ttgaagacgt gcagcgggga gtcgatgtgg
      721 gcgccagtgt gcagactctg gttcacactc gacacctgcc agaagccggg cccgcggcgg
      781 tgcggcgtca cctcgagctt catgttggtg gagggcgggc tgaacgtgtt ctcgtccacc
      841 gtcacggaca ggtcgatgaa gcggctcatg cgtgggtcat ttcgtgaagt aggcgatggc
      901 gcgggcgggc ccggtcagct ccacgatgtg gagcccggtg tcggcccggt ccgccaggta
      961 gatgaacccg cggtcgtcca cgtccacgtt gttggtctgg atcgcggtct tgcagcgggg
     1021 cgggccgccc gcgacctcga cgcagcgctt gtccgtcttg ggagtaaccg acggaatata
     1081 gaagccggcc tcggtcggat ggaacgggtc gcgcacgtcc accgcccgca ctcccgcgtt
     1141 gaagtacgcg atgaacgcga gacgtccgta gtagatcggg ctgaacgact cgttgctcga
     1201 gtgggggccg aagcgcccgc cgcggcggca gaagtcgccg gtgggatccg gcacctcgaa
     1261 ggtggccacc gagtacggcg tgcgctcggt ggtcacgtcc accatgaacg agagctggcg
     1321 gatctcctgg cactcgttgc ggagcgactc ggagaccagc agcacgaagt cgcgggtgcg
     1381 gcccggcacg cccggcgacc agtgcggcac cgaaaccccc aggatcggga agctggtgtg
     1441 cccgccccag gccggcgaca tgtcgagccg gccgatctgg ggatagagca ggttctcggc
     1501 ggtcggcgcg aaccgatcgg cggcctgcgg attgcccgcg agcagcttct cgcggtcgac
     1561 gatctgcagc acgcccttgg ccccggtgcc gtacgcgaag tagacgcggt tccgcagcgc
     1621 gatgggcccg tgcacgccct ccggcgccac gccggtcgat ccgggctcct gcccgggcag
     1681 gccgaagtcg cggatgaagc gcggctgcgc cggatcgccc aggtcgtaga tcttggtcat
     1741 gcggttggtg cgccagccgg acggcgcgcc gtccgagacc aggtaggcga tgccggtgtc
     1801 gcactcccac cagttcttgt gcgtgctctt gaggccgtcg agcaccgtga cgagccgggc
     1861 cggcttcgcg ggatcggtca cgtcccagac ctcgtgcgcg aggttgccga gcgtgcgcag
     1921 caggtaggtc ttctccttct gtgcgcgcgg cagcgtcttg ccgtcgcaga cccgcaccat
     1981 ggccgccccg cccgcttccg cctccccctc cgcgccgggg atgtgatgca ggtagcgcgg
     2041 gtgcttcggg tccgtgacgt ccaggatgga cgtgccgttc agctcctcct gcccggtgag
     2101 cgggttcatc cgcttgccgc cgtggtgacc gaggtaggcg atccagcgcg cgccctggtg
     2161 gtggacgacc ggctggtagg ccgagcgggc ctggaggtcg tccatgccga cgaggcgcat
     2221 gttgcgctgc tcctcggccg gggcggcggg tgcgcacgcg agcagaccgg ccagcatgag
     2281 cgcggcgctc catccgcttc gcgtgctcac ggcgttatgt ccgtcgacat cgacgctcct
     2341 ccctgggtga cccgatgtgg catgaaagcg ggccccaagc ctacccgggc gtccgccggg
     2401 cggtcaatag ccaatcccgg cggccacttc gcggaagcgg cggacgcgtc tcgaccggag
     2461 actagggcgg cggtttacaa tcgcggcatg gccgagccgc gcggtaccgc tagggtcacc
     2521 ttcctggtca acgcggccgg aaatttcctg ggcgcggccg tcgcgttcct gtacttccgc
     2581 ctggtggacc atcacgccgc gagcctgcct cgggtcagcc tgcacgaggt cgtccatccg
     2641 ctcctcgtct tcgcggggct gctcgccctg ggcaactggc tcaacaatcg ttgggtggcc
     2701 ccaatcaacc gggccaccca ggggcacccg ctgtccgagc gagaggccgc gctcgtccgc
     2761 cggcgcgcgc tgctcttccc ctacgtcctg gccggagtct ccttcgcggg ctgggtggtg
     2821 gccggcctga tctggggctt cggcatgccg ctcatgatgg gacgagtcgt ctcgctgtcc
     2881 gacagcgtgc ggatcgtctt cgggatcacc gtgctcgcgg gcaccgtgac taccgccttc
     2941 atcttcttcg tcagcgagcg catctggcgg acgcggctgc tactcctctt cccggaaggc
     3001 gacctgagcg cggtgccgcg cgtgccccgc ctggcggtgc gcgtccggct gctcgccatc
     3061 ttcctgctca ccgcggtagt tccgctcgcg gtgcttggca tcgtggcgta cgcgcgcggg
     3121 ctcgacctgc tcgac
//
LOCUS       DATMCV010000328         2673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_84863, whole genome shotgun sequence.
ACCESSION   DATMCV010000328 DATMCV010000000
VERSION     DATMCV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84863"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..982
                     /locus_tag="VNU03_07645"
     CDS             179..982
                     /locus_tag="VNU03_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP74372.1"
                     /translation="MPHVSIGDADIYYEEQGQGPALMLVPGLGGQGAFWTPQVRDFAR
                     DFRVIVHDHRGAGRSTHSRIRYSVDQMAADTIALMDRLGIARAHYVGHSTGGAMGQTI
                     AQDHPDRLLSLVLSATWPGQDAYFRRCFETRKELLLHRGFESYWRASVVMLRPPRWIS
                     ENDAALAEEMQRLTAAPPPAEVLASRIDAIVAFDRRARLGEIRCPTLVVVAADDALTP
                     LFYSEELAARIPDAKLVVLPTGGHFVPNLLPAEYNRAVGDFLRSHRERS"
     gene            1068..1649
                     /locus_tag="VNU03_07650"
     CDS             1068..1649
                     /locus_tag="VNU03_07650"
                     /inference="COORDINATES: protein motif:HMM:NF025457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4269 domain-containing protein"
                     /protein_id="HWP74373.1"
                     /translation="MGPRDRSWRDPAYLASGSPAQRAAARALAESGVLERLAAFDPVL
                     AGTYPLDLSVAGSDLDILCHAPDLMSFERAVRGAFANRPGFACERQPAGAMLATLARF
                     HSRELPVEVFGQALPVDLQRGYRHLLVEARLLDLGGPRLRRAVRDLREDGMKTEPAFA
                     RALGLRGDPHERLWALSWCDDVELRAILGAGDP"
     gene            1646..1858
                     /locus_tag="VNU03_07655"
     CDS             1646..1858
                     /locus_tag="VNU03_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74374.1"
                     /translation="MTTQPRAVSDTWEQGDPYEQYIGQWSRRLALDADARSRLRELLR
                     ARLPAAADGSISLTARAWAARGAVSA"
     gene            1862..2401
                     /locus_tag="VNU03_07660"
     CDS             1862..2401
                     /locus_tag="VNU03_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74375.1"
                     /translation="MGFLRWLLRRGPRIEAHTLGVLDLSGGTAGALMAADRTALAPLF
                     RRVSESTDAPPRSTLLLVYCTIAADGAILNSARSLREIIRDAGAPVVVVATPNSGQSY
                     TVAANRNKQLARANLVMTLDRKGETFAHFFRRLVADMKQGTSMPRAWVKLAPQTPRGT
                     HPDCPDAIFACELGQLAFS"
     gene            2406..>2673
                     /locus_tag="VNU03_07665"
     CDS             2406..>2673
                     /locus_tag="VNU03_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74376.1"
                     /translation="MTETGLPYDRALLLAGARRNAVLDLEEVRRYGADSYRDPDYVSI
                     YGLPPAEWYARGIRLLGRTAVECTRDDLGDAIGRDVAGIALMAPP"
BASE COUNT          341 a         1030 c          939 g          363 t
ORIGIN      
        1 cgaggacctg gtgatgctct gggtgatcac cccgcccggt ctcgaggatt tcttcgagag
       61 catcgggcgc ccacggcagc ccggcgcgcc cgcccccgcg ccgttcgagc ggccgcagga
      121 cgtgatcgcg atcgagcgcc gcatgggcat gaacgacacg aagtagtcgg cgcgcgccat
      181 gccgcacgtc tcgatcggcg acgccgacat ctactacgag gagcagggcc agggcccggc
      241 gctgatgctg gtccccggcc tgggcggcca gggcgcgttc tggaccccgc aggtgcgcga
      301 cttcgcccgc gacttccggg tgatcgtcca cgaccaccgc ggcgccggcc gcagcaccca
      361 ctcgcgcatc cgctattcgg tcgaccagat ggccgccgac accatcgcgc tcatggaccg
      421 cctgggcatc gcgcgcgcgc actacgtcgg ccactccacc ggcggggcca tgggccagac
      481 catcgcgcag gaccacccgg accggttgct gagcctggtg ctgagcgcga cctggcccgg
      541 acaggacgcc tacttccgcc gctgcttcga gacgcgcaag gagctgctgc tccaccgcgg
      601 gttcgagagc tactggcggg cctcggtggt catgctgcgc ccgccgcgct ggatcagcga
      661 gaacgacgcc gccctcgccg aggagatgca gcggctgacc gcggccccgc cgcccgcgga
      721 ggtgctggcg agccgcatcg acgcgatcgt ggccttcgac cggcgcgccc gcctgggcga
      781 gatccgctgc cccaccctgg tggtggtcgc ggcggacgac gcgctcacgc cgctgttcta
      841 ctccgaggag ctggccgcgc gcatccccga cgccaagctg gtggtgctgc ccaccggcgg
      901 tcacttcgtg ccgaatctcc tgcccgcgga gtacaaccgc gcggtgggcg actttctccg
      961 ctcccaccgc gagcggagct agcgcgccgg tggaccaggt caatctcgcg gccaagctcg
     1021 ccacgttcgg cgaccactgg cagccgcggc gggtcgtcta gccgcgcgtg gggccgcgag
     1081 accggagctg gcgcgatccg gcctatctcg cgtccggctc gccggcgcag cgggcggccg
     1141 cgcgcgccct cgcggagagc ggcgtgctcg agcggctcgc cgcgttcgat cccgtgctgg
     1201 ccggcacgta tcccctcgac ctctcggtgg ccggcagcga cctggacatc ctgtgccacg
     1261 cgcccgacct catgagcttc gagcgcgccg tgcgcggggc attcgccaat cggcccggct
     1321 tcgcctgcga gcggcagccg gccggcgcca tgctcgcgac cctggcccgc tttcactccc
     1381 gggagctgcc ggtcgaggtg ttcggtcagg cgcttccggt ggatctgcaa cgcgggtacc
     1441 ggcacctgct cgtggaggcg cggctgctcg acctcggcgg ccctcgcctg cgccgcgccg
     1501 tccgcgacct gagagaagac ggaatgaaga ccgagcccgc cttcgcccgg gcgctcgggc
     1561 tgcgcggcga tccgcacgag cggctgtggg cgctatcctg gtgcgacgac gtggagctcc
     1621 gcgctatcct gggcgcgggc gacccatgac cacgcagccg cgcgccgtct cggacacctg
     1681 ggagcagggc gacccgtacg agcagtacat cggccagtgg agccgccggc tggcgctcga
     1741 cgcggacgcg cggagccgcc tccgcgagct tctgcgcgcg cgcctgcccg cggcggcgga
     1801 cggctcgatc agcctgaccg cgcgggcctg ggccgcgcgc ggcgcggtct cggcttgagc
     1861 gatgggcttc ctgcgctggc tgctccgccg cgggccccgg atcgaggccc acaccctggg
     1921 cgtgctcgat ctctccggtg gcaccgccgg cgcgctgatg gccgccgatc ggacggcgct
     1981 ggccccgctc ttccggcggg tgagcgagag caccgacgcc ccgccgcgca gcaccctgct
     2041 gctcgtctac tgcacgatcg cggccgacgg cgcgatcctc aactcggcgc ggagcctgcg
     2101 ggagatcatc cgcgacgcgg gcgcgccggt ggtggtcgtg gccaccccca actcgggcca
     2161 gagctacacc gtcgccgcga accgcaacaa gcagctcgcc cgcgccaacc tcgtgatgac
     2221 gctcgatcga aagggcgaaa ccttcgccca cttcttccgg cgcctcgtcg ccgacatgaa
     2281 acagggcaca tcgatgccgc gcgcctgggt gaagctcgcc ccccagactc cgcggggcac
     2341 ccaccccgac tgccccgacg cgatcttcgc gtgcgagctg gggcagctcg ccttctcctg
     2401 accgcgtgac cgagaccggg ctgccttacg atcgtgcgct cctgctcgcc ggcgcccggc
     2461 gcaacgcggt gctcgacctg gaggaggtgc ggcgctacgg cgccgacagc taccgcgacc
     2521 ccgactacgt ctcgatctac ggcctgccgc cggccgaatg gtacgcgcgg ggcatccgcc
     2581 tgctcgggcg caccgcggtc gagtgcacgc gggacgacct gggcgacgcg atcggccggg
     2641 acgtggccgg catcgccctc atggcgccgc cct
//
LOCUS       DATMCV010000329         4573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_419683, whole genome shotgun sequence.
ACCESSION   DATMCV010000329 DATMCV010000000
VERSION     DATMCV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419683"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VNU03_07670"
     CDS             complement(<1..493)
                     /locus_tag="VNU03_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWP74377.1"
                     /translation="MRVLLVHPSPLMYSELYLRLEPLGLERVAAAVRAAGHEVRLLDL
                     QIFSHADYWRELETFAPQAVGFSVNYLANVPEVIDLAKDTKAKRREAFVFVGGHSASF
                     VAAEFLEHAEGAIDCIVRGEGEVIAPRVLEAIGDPKLDTLPGVVTLTGTGPSPTLLDN
                     LDLH"
     gene            777..1202
                     /locus_tag="VNU03_07675"
     CDS             777..1202
                     /locus_tag="VNU03_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP74378.1"
                     /translation="MATIEFNGGVEAGSRYRLLLVDPYGHSREGLGAALRGGGVLVET
                     AEGASQAMAVMQAGEVDLAIVDLDLPPTHGLAEDGWDLVHLLRAASPTLPLILIAAEC
                     RPQTQDEALRLGARLLEKPINPRELRTIVRALRPETAPA"
     gene            1199..1327
                     /locus_tag="VNU03_07680"
     CDS             1199..1327
                     /locus_tag="VNU03_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74379.1"
                     /translation="MKRATIGFGVLVTFFLLAMFLMADAEVPTVDDTAVVTSTSAR"
     gene            1402..1698
                     /locus_tag="VNU03_07685"
     CDS             1402..1698
                     /locus_tag="VNU03_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74380.1"
                     /translation="MATMLIAYPWLVVLLAGWVFGAIFVGMVGGATRGVQGFFVWILS
                     ALIFSPLLAMLGLIAVLLGDLVREVEIKAIHGADELPAPRRSSGRFAHLAGEDD"
     gene            1709..2554
                     /locus_tag="VNU03_07690"
     CDS             1709..2554
                     /locus_tag="VNU03_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HWP74381.1"
                     /translation="MPTIGILHPGDMGSVVGACAVAAGARVLWASEGRSAGSRERAAA
                     AGLEDAGTVAALVAASDVILSVCPPHAALDLARDVAGRGFAGLFVDANAVAPATAREI
                     GAAVEAGGATFVDGGLIGPPPRNAGSTRLYLSGREAKRAAALFEPSALEAIAIGDGAG
                     AASALKMTYAAYTKGSSALLMAIRALAASEGVDDALLAEWRRSQPDLPKRSESAARDN
                     ARKAWRFVGEMEEIAAAFEAAGLPGGFHEAAAVIYERLSGYKDATTPPSMADVVAPLT
                     RPAAR"
     gene            2603..2905
                     /locus_tag="VNU03_07695"
     CDS             2603..2905
                     /locus_tag="VNU03_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWP74382.1"
                     /translation="MVTVALYVQLEAKPGKETEVEAFLKGGLAIVQGEPATTAWFALR
                     LGPTTFGIFDAFPDEAGRQAHLSGRVAAALMQKAGELLAKPPAIEKVDVLAAKLPK"
     gene            3006..3081
                     /locus_tag="VNU03_07700"
     tRNA            3006..3081
                     /locus_tag="VNU03_07700"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3039..3041,aa:Lys,seq:ttt)
     gene            complement(3102..3230)
                     /locus_tag="VNU03_07705"
     CDS             complement(3102..3230)
                     /locus_tag="VNU03_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWP74383.1"
                     /translation="MDDFLYSEPAVVPEPATLLLFGTTAAGLGLARWRRWRHGQQR"
     gene            complement(3412..3798)
                     /locus_tag="VNU03_07710"
     CDS             complement(3412..3798)
                     /locus_tag="VNU03_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP74384.1"
                     /translation="MTGHALVLVVEDEPDVRELIADVLAENGYEIEVAPDGAAALQLS
                     DQRKYDLILSDLRMPKMDGAALYWALQLRHGTAMPRMIYVTGQAHSLDYAGFLAASRV
                     PVLSKPFSPDDLRQAVRQALAKNNGS"
     gene            complement(3857..4225)
                     /locus_tag="VNU03_07715"
     CDS             complement(3857..4225)
                     /locus_tag="VNU03_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74385.1"
                     /translation="MKFAYFTNDAPVAGTLLYTAFRRNMPPELKEFTVRNPVTFFYGI
                     QQTGGLLNIQVRWINPDGVVARTSDQPMDQSREGALWTWQVDRLEQRDLSQAGVWVVE
                     LMVSGHRVGRYPLLVRPLAR"
BASE COUNT          659 a         1574 c         1621 g          719 t
ORIGIN      
        1 tgtggagatc caggttgtcg agcagggtcg ggctcgggcc ggtcccggtc agcgtcacca
       61 ccccgggcag cgtgtcgagc ttcgggtcgc cgatggcctc gagcacccgc ggggcgatga
      121 cctcgccctc gccgcgcacg atgcagtcga tggcgccctc cgcgtgctcg aggaactcgg
      181 cggccacgaa cgacgcgctg tggccgccga cgaacacgaa cgcctcgcgc cgcttcgcct
      241 tcgtgtcctt ggcgaggtcg atcacctccg gcacgttggc caggtagttc accgagaagc
      301 ccaccgcctg cggggcgaac gtctcgagct cccgccagta gtcggcgtgg ctgaagatct
      361 gcagatcgag cagccggacc tcgtggccgg ccgcgcggac cgccgcggcc acccgctcga
      421 ggccgagcgg ctcgagcctc aagtagagct cggagtacat cagaggactc gggtgcacca
      481 gcagcacgcg catagcgtgg accctccttt cgcacgcatg atacgtgaga gctacgcatc
      541 ccgcctcctt cggcttgcat tttggggtgc ctcccaagtc cgcgtcagac agtaaaaatt
      601 actcgtttcc ttgtaacgct tacacgacaa gtgtcagcgc ggtgacgcgg atcgtccagg
      661 cggcgctcag ggtcgaaaag ctaactgatc ggaattctga atgttcggca ccccgcagga
      721 cggcgctttg cgggtggcat tcgggttgcc tacggtagga ccggagcccg acgacaatgg
      781 cgacgatcga gttcaacggc ggtgtggagg cggggagccg gtaccgcctg ctcctcgtcg
      841 atccctacgg ccacagccgc gagggactgg gcgccgcgct ccgcggcggc ggtgtcctgg
      901 tcgagaccgc cgagggggcc tcccaggcga tggccgtgat gcaggccggg gaagtggacc
      961 tggccatcgt cgacctcgac ctcccgccga cccacggtct cgccgaggac ggctgggacc
     1021 tggtccatct cctgcgggcc gcgagcccga ccctgccgct gatcctgatc gcggccgaat
     1081 gccgacccca gacgcaggac gaagcgctcc gcctcggggc gcgcctgctc gagaagccca
     1141 tcaacccgcg cgagctgcgg accatcgtcc gcgccctgcg cccggagacc gcaccagcat
     1201 gaagcgcgca acgatcggtt ttggcgtcct cgtgaccttc ttccttctcg cgatgttcct
     1261 gatggccgac gccgaagtgc cgacggtgga cgacaccgcc gtcgtgacca gcacctccgc
     1321 ccgctgaccg cctgaccgct tcctcccgag cggccgcccg ccgcctactc cttccttgcg
     1381 gactgcggta ccatgccgtc cgtggccacg atgctgatcg cgtatccctg gctcgtcgtc
     1441 ctgctcgccg gctgggtgtt cggtgccatc ttcgtcggga tggtcggcgg ggccacccgt
     1501 ggggtgcagg gcttcttcgt ctggatcctg tccgcgttga tcttctcgcc gctgctcgcc
     1561 atgctcggcc tcatcgcggt gctgctgggc gatctggtgc gcgaggtgga gatcaaggcg
     1621 atccacggag cagacgagct gccggccccg cgccgctcgt cggggcgctt cgcccatctg
     1681 gccggagagg acgactgagg agaggacgat gccgacgatc gggatcctgc atccgggcga
     1741 catggggagc gtggtgggcg cctgcgcggt ggccgcgggg gcgcgggtgc tctgggcctc
     1801 cgaggggcgc agcgcgggca gtcgcgagcg cgcggccgcg gccgggctcg aggacgcggg
     1861 cacggtggcc gcgctggtgg cggccagcga cgtgatcctc tcggtgtgcc ccccgcacgc
     1921 cgcgctcgac ctggcgcgtg acgtggcggg ccgcggcttc gccggcctct tcgtcgacgc
     1981 caacgcggtg gcccccgcga ccgctcgcga gatcggcgcg gccgtcgagg ccggcggcgc
     2041 caccttcgtc gacggcggtc tgatcggccc gcccccgcgg aacgcgggct cgacccggct
     2101 ctacctgtcc gggcgcgagg ccaagcgcgc cgccgccctc ttcgagccga gcgcgctcga
     2161 ggccatcgcc atcggcgacg gagccggcgc cgcctccgcg ctcaagatga cctacgccgc
     2221 ctacaccaag ggctcctccg cgctcctgat ggcgatccgg gcgctggccg catcggaggg
     2281 cgtggacgac gcgctgctcg cggagtggcg gcgctcgcag ccggacctgc ccaagcggtc
     2341 ggagtcggcc gcccgcgaca acgcccgcaa ggcgtggcgc ttcgtggggg agatggagga
     2401 gatcgcggcc gcgttcgagg ccgcgggtct gccgggcggc ttccacgagg cggccgcggt
     2461 gatctacgag cgcctctccg ggtacaagga tgccaccacg ccgccgagca tggcggacgt
     2521 ggtcgccccg ctcacgcgtc cggcggccag gtagcgccgg ctctgcgacg cgcggtctcg
     2581 cgccgcgcaa gggagaaccc acatggtgac cgtcgctctc tacgtccagc tcgaggcgaa
     2641 gcccggcaag gagaccgaag tcgaggcatt cctcaaggga ggcctggcga tcgtgcaggg
     2701 cgagccggcc accaccgcct ggtttgcgct ccggctgggc ccgaccacct tcggcatctt
     2761 cgacgcgttc ccggacgagg cggggcggca ggcccacctc tccggccggg tcgcggccgc
     2821 gctcatgcag aaggccggcg agctcctggc gaagccgccg gcgatcgaga aggtggacgt
     2881 cctcgcggcc aagctgccca agtagcctgc gcgcgtactc cgcgtggcgg cggtggactc
     2941 tgcgggccgg cgcggcgccg tgatagactc cggctcgcgc atcagttcgg tgatgtgacg
     3001 ggcgcgagcc gttagctcag ttggtagagc acctgacttt taatcagggg gtcggtggtt
     3061 cgatcccacc acggctcacc accgctgttg ccgccgccgc tctaccgctg ttgtccgtgt
     3121 ctccaccgcc gccagcgagc cagccccaga cctgccgcag tcgtgccaaa gaggagcaga
     3181 gtggccggct ccggaacgac ggcgggctcg ctgtagagga agtcgtccat cacgatgatg
     3241 tcgatggcgc cactctcgct cggcccgagc gggacattgc tggagcctcc tagagagcgg
     3301 aagggtcgag gaacgccgat tctagctctc gcgaatcgtg ccgccaacta cgccgctatt
     3361 acattttgtc tgcggcgaag acgcagataa gactaacggc tctgacgcct actagctgcc
     3421 gttgttcttg gcgagcgcct gtcggacggc ctggcggaga tcatcggggc tgaacggctt
     3481 cgagagcacg ggcacccgcg aggcggcgag gaagcccgcg tagtcgagcg agtgggcctg
     3541 gccggtgacg tagatcatgc ggggcatcgc cgtgccgtgg cggagctgga gcgcccagta
     3601 gagcgcggcc ccgtccatct tgggcatgcg caggtcgctc aggatcaggt cgtacttgcg
     3661 ctggtcggag agctggagcg cggcggcccc gtccggcgcc acctcgatct cgtagccatt
     3721 ctcggccagc acgtcggcga tcagctcgcg gacgtccggc tcgtcctcca cgaccaggac
     3781 cagggcgtgg ccagtcatat cgtcgagttt agcaaaggcg tgggaggtga gccccatttg
     3841 cttgtggcct cgcgggttag cgggcgaggg ggcggaccag gagcgggtag cggcccaccc
     3901 ggtggcccga gaccatcagc tccaccaccc acacgccggc ctgggagaga tcgcgctgct
     3961 cgagccggtc cacttgccac gtccagagtg cgccttcgcg ggattgatcc atcggctgat
     4021 cgctcgtccg ggccaccacc ccgtcggggt tgatccatcg gacctggatg ttcaacaggc
     4081 cgccggtctg ctggatgccg tagaagaacg tgaccgggtt ccggaccgtg aactccttga
     4141 gctcgggcgg catgttccgc cggaacgcgg tgtagagcag cgtgcccgcg acgggcgcgt
     4201 cgttggtgaa gtaggcgaac ttcaccacac tcgccgcttg gaagcccgag agctggagcg
     4261 cggtcggcag gggccgtccg ggggcttgcc gagactgctc ccgccggggt cggttgcgca
     4321 cgctcgttca gggctgcaag tccaaggcca taggtaagtc tagacgaatc ccaccccttt
     4381 gcccaggacg gtccagagct gcgtccgcct gcgaccggac gcggtgacga atgtggtcgg
     4441 gcggtcgcac gccgcggcgg cagtgacagt gtcaccggct cctgcgtcca tcggacgccg
     4501 gagcgtgcgt gatggacgcg cggcgcgctg tagcggcgcc atcttgcgtc gtgcgtcgga
     4561 tggcaagcgc ctt
//
LOCUS       DATMCV010000330         3165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_550142, whole genome shotgun sequence.
ACCESSION   DATMCV010000330 DATMCV010000000
VERSION     DATMCV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550142"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VNU03_07720"
     CDS             complement(<1..658)
                     /locus_tag="VNU03_07720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP74386.1"
                     /translation="MSQTADVVIVGAGIIGGSVAFHLLERDPGLRVVVLEKEPEPGTG
                     ATSKATGGVRFQFSTEANVRLTQLSYRYFTHAEEILGSPVDFTRHGYLFVTTDPGTLE
                     QCRRNVALQRGLGVASEILSPAQIQTHLASLVTHDLVGGSFCADDGSADPYSMLQAFL
                     GRAKGRGLRLLTGHPVTGLVKSADRVTGVRTPQGEWSAPIVVDCAGPYADQIGEMAGV
                     S"
     gene            complement(672..1400)
                     /locus_tag="VNU03_07725"
     CDS             complement(672..1400)
                     /locus_tag="VNU03_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008883125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HWP74387.1"
                     /translation="MRVQGGTNLYGFSVGILMLDTRFPRIPGDMGNATTFDFPVRYHR
                     VTGASPDRVVRQGQRELLPAFVEGARLLEREGVRAITTNCGFLAKFQQELAASVSVPV
                     FTSSLMLVPLVQRMLPPGRAVGVLTVNAASLHREHMAGAGIAADAPVVVTGLETEKEF
                     TRVLLDDQLILDVEAARQEHLTVAGRLVAEHPEIGAIVLECTNMPPYRADIQAATGLP
                     VFDITTLVRMVHDSVCHGLAPRPA"
     gene            complement(1407..2237)
                     /locus_tag="VNU03_07730"
     CDS             complement(1407..2237)
                     /locus_tag="VNU03_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWP74388.1"
                     /translation="MDPDPSLADFTAAVTRADADIPLARAALLIAAAEQPALDVDKEL
                     ARLDDLAEAAAPRDGQDELRRLHRLREFLFEEQGFAGDRGDYFDPRNSYLNHVLDRRL
                     GIPITLSLVLIEVGRRLGLDMEGVGLPGHFITGIRIGGEHVLLDPFNRGALLTVEACG
                     EVVRRALGRPVRLQAEHFTPVTNRQFLVRVLANLKGIYWRLEAWDKVVRVIDRTLALH
                     PTAAGERRDRGAAWSNMGRLERGAADWERYLTEFPDAADHEQVRGQLRRVRQKIAQLN
                     "
     gene            complement(2237..2896)
                     /locus_tag="VNU03_07735"
     CDS             complement(2237..2896)
                     /locus_tag="VNU03_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWP74389.1"
                     /translation="MTAAEFVVALDEANQAVLRRLEPEATLKPEVSGALNVTNLLKVA
                     LKNEIEATEIASRWLVTTDDVEVKMAFARQAGDEAKHYRMIADRLGELGVDARALDPL
                     AQGFGPLFKYLDSLETTVERVAAGQFTREAVAVVKNRQFIEFCDRAGDRLTATLYREV
                     IEPDERFHQQLGRTLLLKLATTPEAQDAARRAAAHTLALAEELQGAALRTAGIHHAPG
                     C"
     gene            complement(2893..>3165)
                     /locus_tag="VNU03_07740"
     CDS             complement(2893..>3165)
                     /locus_tag="VNU03_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74390.1"
                     /translation="RTPEEIATQLAPEPPALVPALVIVDLTTNGWDYAAIFDALAAAP
                     DVPVLGFTTHALARETQPLHARCRRVVTKETLTQELPGLLQHGLAA"
BASE COUNT          429 a         1098 c         1188 g          450 t
ORIGIN      
        1 tcgagacgcc ggccatctcg ccgatctggt ccgcgtaggg cccggcgcag tcgaccacga
       61 tcggcgcgct ccactctccc tgcggcgtcc gcacgccggt gacgcggtcg gccgacttca
      121 cgaggccggt gaccgggtgc ccggtcagca gtcgcaggcc ccggcccttc gcgcggccga
      181 ggaacgcctg gagcatcgag tacggatcgg cggagccgtc gtcggcgcag aagctgcccc
      241 cgaccaggtc gtgggtgacc agcgaggcca gatgggtctg gatctgggcc gggctcagga
      301 tctcgctcgc cacgccgagc ccgcgctgga gcgccacgtt gcgccggcac tgctcgaggg
      361 tgcccgggtc ggtggtcacg aagaggtagc cgtggcgcgt gaagtcgacc ggcgagccca
      421 ggatctcctc ggcgtgggtg aagtaacggt aggagagctg ggtgaggcgc acgttggcct
      481 cggtggagaa ctggaagcgc acgcccccgg tggccttcga ggtcgcgccg gttccgggct
      541 cgggctcctt ctcgagcacg accacccgca ggcccggatc tcgctcgagg aggtgaaacg
      601 ccaccgagcc gccgatgata ccggcgccca cgatgacgac gtcggcggtc tggctcacgc
      661 gggcggcggg ctcaggcggg tcgcggcgcg aggccgtggc acaccgagtc gtgcaccatc
      721 cggaccagcg tggtgatgtc gaagacgggc aggccggtgg cggcctggat gtcggcgcgg
      781 tagggcggca tgttggtgca ctcgagcacg atcgcgccga tctcggggtg ctcggccacc
      841 agacgcccgg ccaccgtgag gtgctcctgc cgggccgcct ccacgtcgag gatcagctgg
      901 tcgtcgagca ggacccgggt gaactccttt tcggtctcga gcccggtcac caccaccggg
      961 gcgtccgccg cgatgcccgc gcccgccatg tgctcgcgat ggagcgaggc cgcgttcacg
     1021 gtgagcacgc cgaccgcccg gcccggcggc agcatccgct gcacgagcgg caccagcatg
     1081 aggctcgagg tgaagaccgg cacgctcacc gaggcggcga gctcttgctg gaacttggcc
     1141 aggaagccgc agttggtggt gatcgcgcgg accccttccc gctcgagtaa gcgcgcgccc
     1201 tccacgaagg cgggcagcag ctcgcgctga ccctggcgca ccacgcggtc cggcgaggcg
     1261 ccggtgacgc ggtggtagcg caccgggaag tcgaaggtcg tcgcgttgcc catgtcgccc
     1321 gggatgcgcg ggaagcgggt gtcgagcatg aggatgccga cgctgaagcc gtagaggttg
     1381 gttccgccct ggacgcgcac gccgccctag ttgagctgcg cgatcttctg ccgcacccgg
     1441 cgcagctggc cgcgcacctg ctcgtggtcg gccgcgtccg ggaactcggt caggtagcgc
     1501 tcccagtcgg cggccccgcg ctcgagccgc cccatgttgc tccaggccgc gccgcggtcg
     1561 cgccgctcgc ccgccgcggt ggggtgcagc gccagggtgc ggtcgatcac ccgcaccacc
     1621 ttgtcccagg cttccaggcg ccagtagatg cccttcaggt tggcgagcac ccgcaccagg
     1681 aactgccggt tggtcaccgg cgtgaagtgc tcggcctgca ggcgcaccgg ccgcccgagc
     1741 gcgcgccgca ccacctcgcc gcaggcctcc accgtgagga gcgcgccgcg gttgaacgga
     1801 tcgagcagca cgtgctcgcc tccgatccgg atcccggtga tgaagtggcc cgggaggccc
     1861 acgccctcca tgtcgaggcc gaggcgccgc cccacctcga tcagcaccag cgagagcgtg
     1921 atcgggatgc ccagccgacg gtcgaggacg tggttgagat agctgttgcg cggatcgaag
     1981 tagtcgccgc gatcgcccgc gaagccctgc tcctcgaaga ggaactcccg gaggcgatgc
     2041 aggcggcgca gctcatcttg cccgtcgcgg ggggcggccg cctccgcgag atcgtcgagg
     2101 cgggccagct ccttgtccac gtcgagcgcg ggctgctcgg cggccgcgat caggagggcg
     2161 gcgcgggcca gcgggatgtc ggcgtcggcg cgggtgaccg ccgcggtgaa gtcggccagc
     2221 gaagggtcgg gatccatcaa cagccgggcg cgtggtggat gccggcggtc cggagcgcgg
     2281 cgccctgcag ctcctccgcc agcgcgaggg tgtgggcggc ggcgcgccgc gcggcgtcct
     2341 gggcctcggg ggtggtcgcg agcttgagca gcagcgtgcg cccgagctgc tgatggaagc
     2401 gctcgtccgg ctcgatgact tcgcgataga gcgtcgcggt cagtcggtcc cccgcccggt
     2461 cgcagaactc gatgaactgg cggttcttca ccaccgccac cgcctcccgg gtgaactggc
     2521 ccgccgcgac gcgctccacg gtggtctcga gcgagtcgag gtacttgaag agcggcccga
     2581 atccctgcgc gagcggatcg agcgcgcgcg cgtccacccc cagctccccg aggcggtcgg
     2641 cgatcatgcg gtagtgcttg gcctcgtctc ccgcctggcg ggcgaaggcc atttttacct
     2701 ccacgtcgtc ggtggtcacg agccagcgag acgcgatctc ggtcgcctcg atctcgttct
     2761 tgagggcgac cttcaggagg ttcgtgacgt tgagcgcccc gctcacctcc ggcttgaggg
     2821 tggcctccgg ctccaggcgg cggaggaccg cctggttggc ctcgtcgagg gcgacgacga
     2881 actcggccgc ggtcacgcgg cgagcccgtg ctgcaggagc ccgggcagct cctgggtcag
     2941 ggtctccttg gtcaccaccc gccggcagcg ggcgtggagc ggctgggtct cccgggccag
     3001 cgcgtgggtc gtgaagccca gcaccgggac gtcgggggcg gccgcgagcg cgtcgaagat
     3061 ggcggcgtag tcccaaccgt tggtggtgag atcgacgatc acgagcgcgg gcaccagcgc
     3121 gggcggctcc ggtgcgagct gggtcgcgat ctcctccgga gtgcg
//
LOCUS       DATMCV010000331         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_752380, whole genome shotgun sequence.
ACCESSION   DATMCV010000331 DATMCV010000000
VERSION     DATMCV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752380"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VNU03_07745"
     CDS             complement(<1..278)
                     /locus_tag="VNU03_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74391.1"
                     /translation="MWNSILQAVTIRRSRLRVYLDPASTYQRHDSADDKGLPGYFCHL
                     LVRNEGGAPARRCRGRLLDVSVRNPDGSTRPAPGFFAPVFLKWAREPD"
     gene            complement(342..2045)
                     /locus_tag="VNU03_07750"
     CDS             complement(342..2045)
                     /locus_tag="VNU03_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HWP74392.1"
                     /translation="MIRPYWVSEDRWPALGLLLVVVSLTLGMVYLSVLLNRWNNDFFS
                     ALQEKNAVAFRRQLLQVGWLVGSFIVLAVYQLYLNQMLEIRWRRWLTERYLRAWLTDR
                     AYYRMQLVDGEADNPDQRIAEDVQLLISHTLALFIGGLRAIVTLGTFVAILWGLSGRL
                     AVPIGGLTLILPGYMVWVSILYAAGGTWLTDWIGRPLVRLNFDRQRYEADFRFSLVRF
                     RENTEGVALYRGEPDEFRGFRELFEAVVRNWWGIMRRQKRLTTFTSGYSQGAWIVPSV
                     VAAPRYFRGELGLGGLMQTVGAFNQVQDALSFFVVSYKEIADWCAVVERLAGFERALE
                     RMRRAAALGDGVRHVDGDDTRLTVEDVDLHLPDGRPLVTHVNLSLLRGDSVLLGGASG
                     SGKSTLFRAIAGIWPFGRGEIRASPRARVLFLPQRPYLPIGTLRSVVSYPMPAAGVDD
                     ATLREALEAVDLGGLAGRLDEAGHWALQLSPGEQQRIAFARALVQKPDWLFLDEATAA
                     VDEVTEARLYRLVRDRLPGTTLFSVGHRATLRPFHARRLVVERTGNGPASIVEEAATP
                     G"
     gene            2216..>2805
                     /locus_tag="VNU03_07755"
     CDS             2216..>2805
                     /locus_tag="VNU03_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74393.1"
                     /translation="MRQLLARRALRSGISLVGLAVLVVGCASHSLVPKEQAGAIRDRD
                     RALTPHAAAIEDVIRRSGTLGALAFLDAGDGRLVVLPGDTPTDAWARHTAAGPGTAGG
                     PVSVPTVVDFVYRPDVPKAPETVTHAFLQQQETLRARIAALDGELGKLSDALAETRKE
                     TQASLATARDDTQKALDALAGDLAAARKFMLQTAQLG"
BASE COUNT          461 a          985 c         1002 g          357 t
ORIGIN      
        1 aaatccggct cgcgcgccca cttcaggaac acgggggcga agaaccccgg cgccggcctt
       61 gtggagccgt cggggttgcg tacgctcacg tcgagcagcc ggcctcgaca ccgtcgggcc
      121 ggggcgcctc cctcgttgcg aaccaggagg tggcagaagt atcccgggag ccccttgtca
      181 tcggccgagt catgccgctg atacgtcgac gccggatcga ggtagacgcg gagccgcgag
      241 cgccggatcg tcaccgcctg gaggatcgag ttccacaccc cagcagggta cacacgaccc
      301 cggccacgtc gcaaccgggg gccggcgcaa ccgggcggcc cttacccggg agtggccgcc
      361 tcctccacga tggacgcggg accgttcccg gtgcgctcca ccacgagccg gcgggcgtgg
      421 aaggggcgca gcgtcgcccg atggcccacg ctgaacagcg tggtccccgg cagccgatcg
      481 cgcaccaggc gatagagccg cgcctcggtc acctcgtcca ccgccgcggt cgcttcgtcg
      541 agaaagagcc agtccggctt ctggaccagg gcgcgggcga acgcgatgcg ctgctgctcg
      601 ccgggggaga gctggagcgc ccagtggccg gcctcgtcca gccggccggc caggccaccc
      661 agatccaccg cctcgagggc ctcgcgcagc gtggcgtcgt ccacccccgc ggcgggcatc
      721 gggtagctca ccacgctgcg gagggtgccg atcggcaggt acggtcgctg ggggagaaag
      781 aggacgcggg cccggggcga cgcgcggatc tcgccgcgcc cgaagggcca gattccggcg
      841 atcgcccgga agagcgtgct cttgcccgat cccgaggcgc cgcccaggag cacgctgtcc
      901 ccgcggagca gcgagagatt cacgtgggtc acgaggggcc ggccgtcggg gaggtggagg
      961 tccacgtcct cgacggtcag gcgcgtgtcg tcgccgtcga cgtggcggac gccgtcgccc
     1021 agcgcggcgg cgcggcgcat ccgttcgagc gcgcgctcga agccggcgag ccgctcaacc
     1081 accgcgcacc agtccgcgat ctccttgtac gacacgacga agaagctgag cgcgtcctgg
     1141 acctggttga aggccccgac cgtctgcatc aggcccccca ggccgagctc gccccggaag
     1201 tagcgcgggg cggccacgac ggaggggacg atccacgcgc cctgcgagta gccggaggtg
     1261 aacgtggtga gccgcttctg ccggcgcatg atgccccacc agttgcgcac caccgcctcg
     1321 aagagctcgc ggaacccgcg gaactcgtcg ggctcgccgc gatagagcgc cacgccctcc
     1381 gtgttctccc ggaagcgcac caggctgaac cggaagtcgg cctcgtagcg ctgccgatcg
     1441 aagttcagcc ggaccagcgg gcggccgatc cagtcggtca gccacgtgcc ccccgccgcg
     1501 tacaggatcg agacccagac catgtagccg ggcaggatga gggtgagccc accgatcggc
     1561 accgccagcc gcccggagag gccccagagg atggccacga acgtccccag ggtcacgatc
     1621 gcgcgcaggc ccccgatgaa gagcgcgagg gtgtgggaga tcaggagctg cacgtcttcc
     1681 gcgatccgct gatcgggatt gtcggcctcg ccgtccacca gctgcatccg gtagtaggcg
     1741 cggtcggtca gccaggctcg gaggtaccgc tcggtgagcc agcggcgcca gcggatctcg
     1801 agcatctggt tgagataaag ctggtagacc gcgagcacga tgaacgaccc gacgagccag
     1861 ccgacctgga gcagctgccg gcgaaacgcc accgcgttct tttcctgcag ggcgctgaag
     1921 aagtcgttgt tccagcgatt caggaggacg ctcagataga ccatcccgag agtgagcgac
     1981 acgaccacga gcaggagtcc gagcgccggc caccgatcct ccgagaccca gtaggggcgg
     2041 atcatcctcc acgcgtcggg aatggttcgg cggagacgac cgacggaggg atcggtggga
     2101 gcatcggaga gggctgcaga catgattctg ggatgctaca actgtttccc aacccagacc
     2161 accgtgggat gtggtgatag agttcgatga agccgttccg acggaggacg tgacgatgcg
     2221 ccaactgctc gcccgacgcg cgctgcgctc gggcatctcg ctcgtgggtc tcgcggtgct
     2281 ggtcgtcggc tgtgcgagcc actcgctcgt gccgaaggag caggccgggg cgatccggga
     2341 ccgcgatcgc gcgctgaccc cgcacgccgc ggccatcgag gacgtcatcc ggcgatccgg
     2401 caccctgggc gcgctggcct tcctcgacgc cggcgatggt cgtctcgtgg tcctgcccgg
     2461 cgacaccccg accgacgcct gggcgcggca caccgcggcc gggccgggga ccgcgggcgg
     2521 cccggtgtcg gtaccgacgg tggtcgactt cgtgtaccgc ccggacgtgc ccaaggcgcc
     2581 cgagaccgtc acgcacgcgt tcctgcagca gcaggagacc ctccgggcca ggatcgccgc
     2641 cctcgatggc gagctcggca agctctccga tgcgctcgcg gagaccagga aggagacgca
     2701 ggcctcgctc gcgacggccc gagacgacac ccagaaggcg ctggacgccc tcgcggggga
     2761 cctggccgcg gcgcggaagt tcatgctgca gaccgcccag ctggg
//
LOCUS       DATMCV010000332         4349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_350592, whole genome shotgun sequence.
ACCESSION   DATMCV010000332 DATMCV010000000
VERSION     DATMCV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350592"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..431)
                     /locus_tag="VNU03_07760"
     CDS             complement(18..431)
                     /locus_tag="VNU03_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP74394.1"
                     /translation="MSAETSPVLLRAEGLTAGYGKMAILHDVTLEVRAGEMVSVIGPN
                     GAGKSTAFKTIVGFLRPTSGRVRFDGQDITGLRPDQVLPRGLAYVPQGRIVFPQMTVL
                     ENLEMGAYIERDRARIRAALERVYALFPVLAERRS"
     gene            complement(428..1075)
                     /locus_tag="VNU03_07765"
     CDS             complement(428..1075)
                     /locus_tag="VNU03_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP74395.1"
                     /translation="MRSEAIRSVIRAYDDPVVRAYCWGRFWILRQRFLDEIGQYLPVR
                     GRVLDLGCGFGLFSLYYASVHPGLTVEGFDRNARRIGMAQAAAERLGLGNVRYQVGDV
                     MDFRGGERFDAAYMLDIVHHIPEEAVRPLLEQVAKTLPEGGRLLVKDVDRQPAYKRWF
                     THALDRVMDPATPVRYWGAEELQTLLESVGFDVHRHLLVDVLPYPHVLYIARRRS"
     gene            complement(1053..2528)
                     /locus_tag="VNU03_07770"
     CDS             complement(1053..2528)
                     /locus_tag="VNU03_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74396.1"
                     /translation="MKLISAASAVLLAYLVWGSRGWPLIHDAPLMHYIAWLIGQGAVP
                     YRDAFDMNLPGVYLLHLAVLRVGGAGDWAWRLFDLGWLAATCGLLLAYCHRISHGWAA
                     VSAALLFALYHLSGGAWHAGQRDFLLCLFLLLGAWGVARGTEQASSAALCWGGLALGA
                     AVTLKPHAALYWVLCAAIAGRDRSWAMSGWRAAALVIAAGLLVPGAVFGWLAWRGGLG
                     PFLEILTGYVLPLYSRVGHVSPWQAIAWHSFGRSVAGLLLLLTVLALLVPPPREPARK
                     GLALVGVLYGLVHFVVQGKGWEYQLYPLILFCCALAPAAVASWRAVGWSRGLDLFGAR
                     RPIALAAWALLVIVLGAKGVEALDAPWIGEKARRVTAITRDLGPLAPPGATVQVLDTT
                     SGGIHALLRLGIRQPTRFIYDFHFFHDVDDPRIQALRAELVAGLERERPAAVVVLEES
                     WPELGYARLAAFPELARTLDRSFTLAVTGAGYRIYAKRSDS"
     gene            complement(2506..3210)
                     /locus_tag="VNU03_07775"
     CDS             complement(2506..3210)
                     /locus_tag="VNU03_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74397.1"
                     /translation="MAPILEIRSAIKRFGGVAAVNRVSLALEPGRIYGLIGPNGSGKT
                     TLFNCITGVDHVDEGEILLKGRRVDRLKPHQIFHLGVGRTFQVIRVFPELTALENLMV
                     VTTGGYPEARRRAQELLALVKLERLADEFAGNLSYGQQKLVEFIRVLMTDPELILLDE
                     PAAGVNRTLLNELLDAVRRLRDQGKTVLLVEHDMKVVMGLCETVFVLDHGEKLAEGAP
                     GVIQTDERVIEAYFGR"
     gene            complement(3217..4134)
                     /locus_tag="VNU03_07780"
     CDS             complement(3217..4134)
                     /locus_tag="VNU03_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74398.1"
                     /translation="MKRWFPVALGLLAVGLALYPLEATGYGVRVGLQLFMWIALAQSW
                     NLISGLTGYVSFGHVVFFGTGAYASSLLVNAGLPWPVACLGGGVAGAVLALIIGWPCL
                     RLKGPYFAIAMLGLNEVMRVIVSYAEGLTGGGNGLSLPTLDASRSIYYAMGLVAAAVT
                     AVTYAIVTSRFGLRLMTIREDEVAAEAMGIDTLRYKIYAFMLSAIGPGIVGGLSARDQ
                     GYIEPISVFPLIMTITMIVMALFGGKGTIWGPVLGAVVLFIFQEAVWARFVYLHQLLF
                     GAIIVAVVLMMPRGILGVLQQKYNLPRTI"
     gene            complement(4131..>4349)
                     /locus_tag="VNU03_07785"
     CDS             complement(4131..>4349)
                     /locus_tag="VNU03_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74399.1"
                     /translation="EMGQVYTFKSFLVIVLGGAGNYPGALLGGLLLGLVEQLSSLFLT
                     AQVNEAVAYVLLVLVLLIRPTGLLKGRV"
BASE COUNT          747 a         1532 c         1547 g          523 t
ORIGIN      
        1 gacatggtgc cggccttcta gctccgccgc tcggccagga ccgggaacag ggcgtagacg
       61 cgctcgagcg cggcccggat gcgcgcccgg tcacgctcga tgtaggctcc catctcgagg
      121 ttctcgagta ccgtcatctg cgggaacacg atgcgcccct ggggcacgta ggcgaggccc
      181 cggggcagca cctggtcggg tcggagcccg gtgatgtcct ggccgtcgaa gcgcacccgg
      241 ccgctggtgg gtcgcaggaa gccgacgatg gtcttgaacg cggtcgactt gcccgcgccg
      301 ttgggcccga tgacgctcac catctcgccg gcgcgcacct cgagggtgac gtcgtggagg
      361 atggccatct tgccgtagcc cgcggtcagg ccctcggcgc gcaggaggac cgggctcgtc
      421 tccgcgctca cgaacggcgc cgggcgatgt agaggacgtg cggatagggc agtacgtcga
      481 ccagcagatg gcggtgcacg tcgaagccca cgctctcgag cagcgtctgc agctcctcgg
      541 cgccccagta gcggaccggc gtggccggat ccatcacgcg gtccagcgcg tgggtgaacc
      601 agcgcttgta ggccggctgc cgatccacgt ccttgacgag cagccgcccg ccttccggca
      661 gggtcttggc cacctgctcg aggagcgggc gcaccgcctc ctccgggatg tggtgcacga
      721 tgtcgagcat gtaggcggcg tcgaaccgct ccccgccgcg gaagtccatc acgtcgccca
      781 cctggtagcg cacgttgccg agtccgaggc gctccgcggc ggcctgcgcc atgccgatcc
      841 gccgcgcgtt gcggtcgaag ccctcgaccg tgaggcccgg gtgcacgctc gcgtagtaga
      901 gcgagaagag cccgaagccg cagcccagat cgagcacgcg gccgcgaacg ggcaggtact
      961 ggccgatctc gtcgaggaag cgctggcgca ggatccagaa gcggccccag cagtaggcgc
     1021 gcaccaccgg atcgtcgtag gcgcggatga cgctacgaat cgcttcgctt cgcatagatc
     1081 cggtatcccg cgccggtgac cgccagggtg aaggagcgat cgagcgtccg cgccagctcg
     1141 gggaaggcgg ccaggcgcgc gtagcccagc tcgggccagc tctcctcgag gaccaccacc
     1201 gcggcgggac gctcccgctc gaggccggcc accagctcgg cgcgcagcgc ctggatgcgc
     1261 gggtcgtcca cgtcgtggaa gaagtggaag tcgtagatga agcgggtggg ctgccggatg
     1321 cccaggcgca gcagggcgtg gatgccgccc gacgtggtgt cgagcacctg cacggtggcg
     1381 cccggcgggg ccagcggccc caggtcgcgc gtgatggcgg tcacccggcg ggccttctcc
     1441 ccgatccagg gcgcgtcgag cgcctccaca cccttggccc cgagcacgat caccaggagg
     1501 gcccacgccg ccagcgcgat cggacggcgc gcgccgaaga ggtcgagccc gcgggaccag
     1561 ccgaccgccc gccacgaggc caccgccgcc ggggccaggg cgcagcagaa caggatcaac
     1621 gggtagagct gatactccca gcccttcccc tggacgacga agtggacgag cccgtagagc
     1681 acgcccacca gggccagtcc cttgcgcgcg ggctcgcgcg ggggcggcac gagcagggcg
     1741 agcacggtca ggagcagcag cagcccggcg accgaccggc cgaaggagtg ccacgcgatc
     1801 gcctgccacg gcgagacgtg gccgacacgg ctgtagagcg gaagcacgta gccggtcagg
     1861 atctcgagga acggccccag gcccccgcgc caggcgagcc agccgaagac cgcgcccggc
     1921 accagcagcc cggccgcgat cacgagcgcg gccgcgcgcc agcccgacat ggcccaggat
     1981 cgatcccgcc ccgcgatggc cgcgcagagc acccagtaga gcgcggcgtg cggcttgagc
     2041 gtcaccgccg ccccgagcgc gagaccgccc cagcagagcg ccgcggagga agcctgctcg
     2101 gtgccccggg cgacgcccca ggcgccgagc aggagaaaga ggcagagcag gaagtcgcgc
     2161 tggcccgcgt gccacgcccc gccgctcagg tgatagagcg cgaagaggag cgccgcggag
     2221 acggcggccc agccgtggct gatgcgatgg cagtacgcga ggagcaggcc gcacgtcgcg
     2281 gcgagccagc ccagatcgaa gagccgccag gcccaatcgc cggctccgcc cacgcgcagc
     2341 accgccaggt gcaggagata gaccccgggc aggttcatgt cgaaggcgtc gcgataaggc
     2401 accgccccct gcccgatcag ccacgcgatg tagtgcatca gcggcgcgtc gtggatcagg
     2461 ggccagccgc gcgagcccca gaccaggtac gcgagcagga cggcgctagc ggccgaaata
     2521 agcttcaatg acccgctcgt cggtctggat gacgcccggc gctccctcgg cgagcttctc
     2581 gccgtgatcc agcacgaaca ccgtctcgca gagccccatc accaccttca tgtcgtgctc
     2641 gaccagcagg acggtcttgc cctggtcgcg caggcggcgc accgcgtcca gcagctcgtt
     2701 gagcagggtc cggttgacgc ccgcggccgg ctcgtcgagc aggatcagct cgggatcggt
     2761 catgagcacc cggatgaact cgacgagctt ctgctggccg taggagaggt tgcccgcgaa
     2821 ctcgtcggcg aggcgctcga gcttcaccag ggcgagcagc tcctgggcgc gccgccgggc
     2881 ctccgggtag ccaccggtgg tcaccaccat caggttctcg agcgcggtca gctccgggaa
     2941 gacccggatc acctggaacg tgcggcccac tccgaggtgg aagatctggt gcggcttcag
     3001 ccgatcgacg cggcggccct tcaggaggat ctcgccctcg tcgacgtggt cgaccccggt
     3061 gatgcagttg aagagggtcg tcttgccgga gccgttgggg ccgatgagcc cgtagatgcg
     3121 ccccggctcg agggccagcg acacgcggtt gaccgcggcg acgccgccga agcgcttgat
     3181 cgcggagcgg atctcgagga tgggggccac gggcgactag atggtccggg gcagattgta
     3241 cttctgctgc agcacgccca ggatcccgcg cggcatcatc agcaccaccg ccacgatgat
     3301 cgccccgaac aggagctggt ggaggtagac gaaccgcgcc cacaccgctt cctggaatat
     3361 gaacagcacc accgccccga gcaccgggcc ccagatcgtg cccttcccgc cgaagagcgc
     3421 catcacgatc atggtgatgg tcatgatcag ggggaagacc gagatgggct cgatgtagcc
     3481 ctggtcgcgc gcggagagcc cgcccacgat gccggggccg atcgcggaga gcatgaacgc
     3541 gtagatcttg tagcggaggg tgtcgatccc catcgcctcc gcggccacct cgtcctcgcg
     3601 aatggtcatg agccggagcc cgaagcgcga ggtgacgatc gcgtaggtga ccgcggtcac
     3661 cgccgccgcg accaggccca tggcgtagta gatggaccgg ctcgcgtcga gggtgggcag
     3721 ggagagcccg ttgccgccgc cggtgaggcc ctcggcgtac gacacgatca cccgcatcac
     3781 ctcgttgagt ccgagcatcg cgatcgcgaa gtagggaccc ttgagccgca ggcacggcca
     3841 gccgatgatc agcgcgagca ccgcgcccgc cacgccgccg cccaggcagg cgaccggcca
     3901 cggcaggccc gcgttcacga ggaggctcga ggcgtaggcc ccggtgccga agaacaccac
     3961 gtgcccgaag gacacgtagc cggtcagccc cgagatcagg ttccaggact gggcgagcgc
     4021 gatccacatg aagagctgga gccccacccg caccccgtag ccggtggcct cgagcgggta
     4081 gagggccagg cccaccgcca gcagcccgag cgcgaccggg aaccagcgct tcacacgcgg
     4141 cccttgagca gcccggtggg ccggatcagc agcaccagca ccagcaggac gtaggcgacc
     4201 gcctcgttga cctgcgcggt gaggaacagc gaggagagct gctcgaccag cccgagcagg
     4261 agcccaccca ggagcgcgcc cgggtagttg ccggcgccgc ccaggacgat gaccaggaac
     4321 gacttgaagg tgtagacctg gcccatctc
//
LOCUS       DATMCV010000333         2977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_550260, whole genome shotgun sequence.
ACCESSION   DATMCV010000333 DATMCV010000000
VERSION     DATMCV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550260"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VNU03_07790"
     CDS             complement(<1..1133)
                     /locus_tag="VNU03_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWP74400.1"
                     /translation="MADDKVFTASADPRHAGASIPDQVPILPLRDTVLFPQSALPLGA
                     GRESSVRLLEDVVRGNRLIGVFTQRDASTEEPQEADLHRIGTLATVHKILKQPDGSVR
                     LVVQGPHRIRIVELSQVRPYLVARIAEEPELSPPAGDLETEALARNAVNLFRQVVELS
                     PMLPDELTTVVQNVTQLDRLGDLIAATLPALTTQAKQELLETPDVKARLARLVAVLTK
                     EAEVLALGSKIQSQVESEVGKSQREYYLREQLKAIQKELGATDDRTQELDELREKVEA
                     AGMTEEAKKEALRELDRLSKMPPAAAEYTVARTYLDWLLALPWSKQTTDNLDIAEARR
                     VLDADHSGLEKVKDRILEYLAVKSMRPAGKDPILCFVGPPGVG"
     gene            complement(1154..1540)
                     /locus_tag="VNU03_07795"
     CDS             complement(1154..1540)
                     /locus_tag="VNU03_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWP74401.1"
                     /translation="MTERSRPLYMIGVVAEMVKIHPQTLRMYEKKGLIRPSRTEGKTR
                     MYSAEDVEEIARVTRLARDLGVNLAGIEIILKMRRRMLDMQARIEELTDHVRGDMAEV
                     RDETRRGKGEELVRAASSQLSPVDLF"
     gene            complement(1537..2532)
                     /locus_tag="VNU03_07800"
     CDS             complement(1537..2532)
                     /locus_tag="VNU03_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWP74402.1"
                     /translation="MRDYYAVLEVEAGATAAQIRRAYQRLARRYSPDVNLWEREARAL
                     FEEIAEAYRILGDPTTRIVYDRHGAQPAGRARAGTNEGRRGDDVHVPVELSFQQAVAG
                     VEVDVPVDRLSPCAACGATGAAPGAEPSACSHCGGLGTVWREASGLGVRCPACDGAGA
                     RVSAPCSACRGRGVSTSRASIHVLLPPGLDTGAQLRVAGEGHAGPFGGSRGDLIVVAR
                     VHEDPRYTRKGDNLYCEVPLTLVEAVLGSRVRVRGVDGVVDLAIPPGTQSGQTFRLRG
                     KGVRRLASDGRGDLYVTTRVEIPRDVDTRTQEMFRELGRLLPGRPAVENPGSARE"
     gene            complement(2602..>2977)
                     /locus_tag="VNU03_07805"
     CDS             complement(2602..>2977)
                     /locus_tag="VNU03_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWP74403.1"
                     /translation="NAADDARQRESIEVKNQGDSTVYNTEKMLRDYGDKVDATDKQTA
                     ETALTELREALKTDDLERIKKSQEAVTQASHKLAEAMYKDAQAKTQPGAAGGPQPGAA
                     ADGGAPKGDVVDAEFEDLGEKK"
BASE COUNT          406 a         1098 c          968 g          505 t
ORIGIN      
        1 ttgcccactc cgggagggcc cacgaagcac aggatcgggt ccttgccggc cgggcgcatc
       61 gacttcacag ccaggtactc gaggatccgg tccttgacct tctccagacc tgagtgatcg
      121 gcgtcgagca cccgccgcgc ctccgcgatg tccaggttgt cggtcgtctg cttgctccac
      181 ggcagtgcga gcagccagtc gaggtacgtc cgcgccaccg tgtactccgc ggcggcgggt
      241 ggcatcttgc tgagccggtc gagttcgcgc agggcctcct tcttggcctc ctcggtcatc
      301 ccggccgcct cgaccttctc ccggagctcg tcgagctcct gggtgcggtc gtcggtcgcg
      361 ccgagctcct tctggatcgc cttgagctgc tcccggagat agtactcgcg ctggctcttg
      421 ccgacctcgg actccacctg cgactggatc ttcgacccga gcgccaggac ctcggcttcc
      481 ttggtcagca ccgcgaccaa gcgcgccagg cgcgccttga cgtcgggcgt ctccaggagc
      541 tcctgcttgg cctgggtcgt cagcgcgggc agcgtggccg cgatgaggtc gccgagccga
      601 tcgagctggg tgacgttctg cacgacggtc gtcagctcgt ccggcagcat cggcgacaac
      661 tcgaccacct ggcggaagag gttcaccgcg ttccgcgcga gggcctccgt ctccaggtcg
      721 ccggccggcg gagagagctc aggctcttcc gcgatccgag ctacgagata cggacgcacc
      781 tgcgacagct cgacgatacg gatccgatgc ggaccctgga cgaccagccg caccgacccg
      841 tccggctgct tgaggatctt gtgcacggtg gcgagcgtcc cgatccgatg cagatccgcc
      901 tcctgcggct cctccgtcga ggcgtcccgc tgggtgaaga cgccgatcag gcggttcccc
      961 cggaccacgt cttcgagcag gcggaccgag gactctcgcc ccgcgccgag cggcagagcg
     1021 gactgcggga acagcacggt gtcacgcaac ggcaggatcg gaacctggtc gggaatcgaa
     1081 gcacccgcgt gccgcggatc ggcgctcgcg gtgaacacct tgtcgtctgc catggtgatg
     1141 gccttccttt ccgtcaaaag agatcgacgg gggagagctg gctcgaagcc gcccggacga
     1201 gctcttcgcc tttgccgcgc cgcgtttcgt cccgcacttc cgccatgtca ccccgtacgt
     1261 gatcggtcaa ttcctcgatc cgcgcctgca tgtcgagcat ccggcgcctc atctttagga
     1321 tgatctcgat gcctgcgaga ttcaccccga gatcgcgcgc gagccgggtg acgcgggcga
     1381 tctcctcgac gtcctccgcc gagtacatcc gcgtcttccc ttcggtgcgg ctcggacgga
     1441 tgagtccctt cttctcgtac atccggaggg tctggggatg gatcttcacc atctccgcga
     1501 ccaccccgat catgtagagg ggccggcttc gctcggtcat tcccgtgcgc tccccgggtt
     1561 ctcgacggcg ggccgcccgg gaagcaggcg ccccagctct cggaacatct cctgggtccg
     1621 ggtgtcgacg tcgcggggga tctccacccg ggtggtgacg tagaggtcgc ctcgaccgtc
     1681 gctcgccagg cgccggacac ccttgccgcg caggcgaaac gtctggccgc tctgggtccc
     1741 gggaggaatc gccaggtcca cgacgccgtc cactccccgg acgcgcaccc ggctacccag
     1801 caccgcctca accagcgtca ggggcacctc gcagtagagg ttgtccccct tgcgggtgta
     1861 tctcggatcc tcgtggacgc gggcaacgac gatcagatct ccccgcgacc cgccgaacgg
     1921 gccggcgtga ccctctcccg cgacccgcag ctgggcgccg gtgtcgagac ccgggggcag
     1981 caggacgtga atcgacgcac ggctcgtcga gaccccgcgg ccgcgacacg ccgagcaggg
     2041 cgcggacacc cgggcgccgg caccgtcaca ggcggggcac cggacgccca gtccggaggc
     2101 ctcgcgccag acggtgccga gcccgccgca gtgcgagcac gcgctcggct cggctcccgg
     2161 agcggccccg gtcgccccgc aggctgcaca cggggacaat cggtcaaccg gcacgtccac
     2221 ctcgactccg gccaccgcct gctggaacga cagctcgacc ggaacgtgga cgtcatcgcc
     2281 ccggcgcccc tcgttcgtgc cggcccgggc acggccagcc ggctgggccc cgtgtcgatc
     2341 gtagacgatc cgggtcgtgg gatcacccag gatgcggtat gcctcggcga tctcctcgaa
     2401 cagcgcgcgc gcctcgcgct cccacaagtt gacgtccggg gagtaccggc gcgccagacg
     2461 ctggtaggca cgccggatct gcgccgcggt cgcgccggcc tccacctcga gcaccgcata
     2521 gtagtcgcgc atggtcacgc gcggggctca cgcgatcgcg cgagccccgc gctcctcaat
     2581 gccgtccgta ccggcccggc gttatttctt ctcgcccaga tcctcgaact cggcatccac
     2641 gacgtcgccc ttcggcgcac caccatcggc ggcggcgccg ggctgaggcc cgcccgcggc
     2701 tccgggctgg gtcttggcct gggcgtcctt gtacatcgcc tcggccagct tgtgcgacgc
     2761 ctgggtcacc gcctcctgcg acttcttgat ccgctccaga tcgtcggtct tcagcgcctc
     2821 ccgcagctcg gtcagtgcgg tctcggccgt ctgcttgtcg gtggcgtcga ccttgtcccc
     2881 gtagtcgcgg agcatcttct cggtgttgta gaccgtcgag tcaccctggt tcttgacctc
     2941 gatgctctcg cgctgacggg catcgtcggc cgcgttc
//
LOCUS       DATMCV010000334         3047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_952287, whole genome shotgun sequence.
ACCESSION   DATMCV010000334 DATMCV010000000
VERSION     DATMCV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952287"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VNU03_07810"
     CDS             complement(<1..1514)
                     /locus_tag="VNU03_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP74404.1"
                     /translation="MAGRVRSGKTKRKAARPPSRRAPAQPAGERGASSRRALEQSLAE
                     ALEQQAATSNILKVISGSPTALQPVLDAVVENAARFCGADDASLFQVDGDHLRGDAHL
                     GPVHQPPGLQLPIARGSLGGRTVLERRTIHVADLQAETEEFPDASANARRFGFRTTLS
                     VPLVRDGVAIGVIQLRRGAVSPFSDKQIGLLQTFADQAVIAIENVRLLTELGARNREL
                     TDSLTQQTATAEILRVISSSPADLQPVLDTVVESAARYCGATDAQIWRRDGDGLRLVA
                     HHGPLASPRGRVLPITRETITARSVLDQRVVHVPDIEAVAAEFPRTVHHARELGFYAS
                     VAVPLMREGAAIGALQLRRGETGPFTDKQIELLHTFADQAVIAIENVRLFRALEARTG
                     ELVRTVEQLTALGEVGRAVGSTLDLATVLTTIVSRAVELSGLDGGVVFEYDEATEEFV
                     HRAAAETGGALAAARRSIRIAKGEGAIGRTAITREPVQVPDITVAGAYESRIRD"
     gene            1637..>3047
                     /locus_tag="VNU03_07815"
     CDS             1637..>3047
                     /locus_tag="VNU03_07815"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWP74405.1"
                     /translation="METFTAEERAALAPYFTELDGPVFALVNLPEVVKGALFARYSRS
                     AKSLRRLFLDEFLEPIGRPAEAGSIGTARADRLYERVFSEFGDDSVAQLGGVHLACEG
                     ASNILTKVLEWGRLMAYLEQSTRYVPYTDRPGGRFKYHVPAELDGLAVRERYVEVLDR
                     AFETYTRWIDPMREYWMRRFPQPAGESDAAYRMTIRAKALDSLRGLLPAATRSNVGIF
                     GTGQGYEALLLRMRAHPLREVRDYGALMLVELRKVIPAFLLRLERPERGGVWARYLEE
                     TREATASVAARLLEDVEPEPRPEVTLTDFDPDGEVKVVAAALYAVSALPDDQLSAVAR
                     KLGDEARREVLDAYVGRRENRRHKPGRAFERTVYRFDVLGDYGAFRDLQRHRLLTLEW
                     QPLSTRHGYVTPDELAEAGVLPEWARVMEDSAALHDALAAAVPAAAAYAVPMAYRVRF
                     YMELNAREAMHMIELRTAPQ"
BASE COUNT          398 a         1081 c         1114 g          454 t
ORIGIN      
        1 ttgtctcgga tccggctctc gtacgcgccc gccaccgtga tgtcgggcac ctgcaccggc
       61 tcccgggtga tcgcggtgcg cccgatcgcg ccctcgccct tcgcgatccg gatgctgcgc
      121 cgggctgccg cgagggcccc accggtctcg gcggcggccc ggtggacgaa ctcctcggtg
      181 gcctcgtcgt actcgaacac gactcctccg tcgagcccgg agagctcgac cgcgcgggag
      241 acgatggtgg tcagcacggt ggccaggtcg agggtcgagc cgaccgcacg gccgacctcg
      301 cccagcgcgg tgagctgctc caccgtgcga accagctccc cggtgcgggc ctccagcgcc
      361 cggaacagac ggacgttctc gatcgcgatg accgcctggt ccgcgaaagt gtgcagcagc
      421 tcgatctgct tgtcggtgaa cggtccggtc tcaccgcggc ggagctggag ggcgccgatc
      481 gccgcgccct cgcgcatgag gggcacggcc accgacgcgt agaagccgag ctcccgggcg
      541 tggtgcacgg tccgaggaaa ctcggcggcc acggcttcga tatcgggcac gtggaccacg
      601 cgttgatcga gcacgctccg ggcggtgatg gtctcgcgcg tgatcggcag gacccgcccc
      661 cgcggggagg cgagtggtcc gtggtgggcg accagccgca gcccgtcgcc gtcccgccgc
      721 cagatctggg cgtcggtggc gccgcagtag cgggccgcgc tctccaccac cgtgtccagc
      781 accggctgca gatcggcggg tgagctgctg atcacgcgaa ggatctcggc ggtggcggtc
      841 tgctgggtga ggctgtcggt gagctcccgg ttgcgggccc ctagctcggt gaggagacgg
      901 acgttctcga tcgcgatcac cgcctggtcc gcgaaggtct gcaggagacc gatctgcttg
      961 tcgctgaacg ggctcaccgc gccgcgccgc agctggatga ccccgatcgc cacgccgtct
     1021 cgcacgagcg gcacgctgag cgtggtccgg aagccaaacc tccgcgcgtt cgcgctagcg
     1081 tcggggaatt cctccgtctc cgcttgcagg tccgcgacgt ggatggttct ccgctcgagc
     1141 acggtgcggc ccccgagcga gcctcgcgcg atgggcagct ggaggccggg cggctggtgg
     1201 accgggccga ggtgcgcgtc accgcgcagg tgatcaccgt cgacctggaa taacgaggcg
     1261 tcgtccgccc cgcagaagcg ggccgcgttc tcgaccaccg cgtcgagcac cggctgcagg
     1321 gcggtggggg acccgctgat caccttcagg atgttggagg tggcggcctg ctgctcgagg
     1381 gcctcggcca gggattgctc cagcgcgcgc cgggacgagg ctccgcgctc tccggccggt
     1441 tgtgcgggcg cacggcggct cggcgggcgc gccgccttgc gcttcgtctt gccggatctg
     1501 acccgccccg ccatcggttc tcctcgcctt tcgcggaaag cgcaacgtcc cgcggtcggc
     1561 gataggctag gggaagatgt ccaccggagc aagggccggc cccggggact cgaggccgcg
     1621 tgctaggatg accgccgtgg aaaccttcac cgccgaggag cgggcggccc tggcgcccta
     1681 cttcaccgag ctcgacggcc ccgtcttcgc cctggtcaac ctgccggagg tcgtcaaggg
     1741 cgcgctcttc gcgcgctact cgcgatcggc caagtcccta cgccggctct tcctggacga
     1801 gttcctcgag ccgatcgggc ggccggcgga ggcgggttcc atcggcaccg cgcgcgcgga
     1861 tcggctctac gagcgggtct tcagcgagtt cggcgacgat tcggtggccc agctgggcgg
     1921 agtgcacctg gcctgcgagg gcgcgtccaa cattctgacc aaggtgctcg agtgggggcg
     1981 gctcatggcc tatctcgagc agtccacccg ctacgtgccg tataccgacc gcccgggcgg
     2041 gcgattcaag taccacgtgc ccgccgagct ggacgggctc gccgtgcgcg agcgctacgt
     2101 ggaggtcctg gatcgcgcgt tcgagaccta cacccgctgg atcgatccga tgcgcgagta
     2161 ctggatgcgg cgcttcccgc agccggcggg ggagagcgac gcggcctacc gcatgacgat
     2221 ccgggcgaag gcgctcgaca gtctccgcgg gctcttgccc gcggcgaccc ggtcgaatgt
     2281 gggcatcttc gggaccggcc agggttacga ggcgctcctg ctccgcatgc gggcccaccc
     2341 gctccgggag gtgcgcgact acggggcgct gatgctggtc gagctgcgga aggtgattcc
     2401 cgcgttcctc ctgcgcctcg agcggccgga gcgtggcggc gtctgggcgc ggtacctgga
     2461 ggagacgcgc gaggccaccg cctcggtggc ggcgcgcctg ctcgaggacg tcgagcccga
     2521 gccccggccc gaggtcacgc tcaccgactt cgatccggac ggggaggtga aggtggtggc
     2581 cgccgcgctc tacgcggtct ccgcgcttcc tgacgaccag ctctcggccg ttgcgcgcaa
     2641 gctcggcgac gaggcgcgcc gcgaggtgct cgacgcctac gtcggccgtc gcgagaaccg
     2701 tcgccacaag ccgggccggg cgttcgagcg caccgtctac cgcttcgacg tgctcggcga
     2761 ctacggcgcc ttccgggacc tgcagcgcca ccggctgctg acgctcgagt ggcaaccgct
     2821 ctcgacccgg cacggctacg tgacgcccga cgagctcgcg gaagcggggg tgctcccgga
     2881 gtgggcccgc gtgatggagg actcggccgc gctccacgac gccctcgcgg ccgccgtgcc
     2941 cgccgcggcc gcctacgcgg tgccgatggc ctaccgggtg cggttctaca tggagctgaa
     3001 cgcgcgcgag gccatgcaca tgatcgagct ccgcaccgcg ccgcagg
//
LOCUS       DATMCV010000335         6310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_85247, whole genome shotgun sequence.
ACCESSION   DATMCV010000335 DATMCV010000000
VERSION     DATMCV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85247"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..887
                     /locus_tag="VNU03_07820"
     CDS             336..887
                     /locus_tag="VNU03_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74406.1"
                     /translation="MPSGLLGGAQRCLALALAVSGLSVACTIDPPAGTPPPPMSGVLE
                     RGPRETAFGRTGDEVVNNPQLRDKIRSMFGTDWAPGGKLVRGAMPYLERTEAPRGVRI
                     GDRNYVAVTGCVPATCRTDRVVLLIQEGGDALLARLDEGGFSHYYAYGGDWGLAGSTA
                     RIVDSGLRALERGGDPYPPYPRS"
     gene            1044..1850
                     /gene="otsB"
                     /locus_tag="VNU03_07825"
     CDS             1044..1850
                     /gene="otsB"
                     /locus_tag="VNU03_07825"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWP74407.1"
                     /translation="MSDLARILGWVGDWVAGDGRLLLMTDYDGTLTPIVDDPADAILA
                     EETREQLLRIARSARGSVAVISGRGLEDLKTRVSIPEVVYAGCHGLEIEGPGLAFRHP
                     DALAQQPMLWAVGEELLRRAPTVAGMRIEAKGLAVAVHYRHVAPDEIRRVEIELARAI
                     REQGSCLKIFHGTKVIEVLPQVAWTKGECARWIQEHVLPAGSGPALWLYLGDDWTDEH
                     AFEVLKGLALTIRVGDTVPASKAAYRLRDVGEVRELLGALAEGATAGSRV"
     gene            1847..4102
                     /locus_tag="VNU03_07830"
     CDS             1847..4102
                     /locus_tag="VNU03_07830"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWP74408.1"
                     /translation="MKFQLRLVAGLWLGSLLVVGAFAYFQFVEDRQRLTGELDRRAAL
                     VSEGLKEVLDPALGPRVSKPQVDRLIKKFSKPDLGLAVYDRVASLIAATPDVTKQLEN
                     PPPEATWALTSGAVQTGFRKVGGTMRYVYADPILREDKPSGALVIFLDASDLQTAEWE
                     LWRLNAIRFVVLAVALALIALLVVRMSLTQPLAKMARWTKAVRRGHAIDPPELPDESL
                     FGPIAREVTVLAKNLQRARAAAEEEAALRLIGQTLWTEERLKQFAKMRLGERPLVVVS
                     NREPVSHVWKEGQIQVVTPASGLVTAMDPVMRACGGVWVAQASGDADRETADARGRLR
                     VPPDDPRFTLKRVWLTPEEEAGYYYGFSNEGLWPLCHIVHNRPLFRPEDWAQYRAVNE
                     KFAAAVLEEIAGTESPMVLIQDYHFALLPGLIKRERPDARTAIFWHIPWPNFEAFSIC
                     PWQDELLLGMLGADIIGFHTQYYCNNFLDTVERAIEARIDWERFSVTRGQHVTSVKPF
                     PISVAPGFVDNPPATSRQELLASLDITAEFIGVGVERLDYTKGLPERFRALGRFFERF
                     PEYRERLVFVQLAAPSRSTIPRYQALEAEVDAAVQEVNGAYQTKRWKPILYLKRHHEH
                     RDIWPFYRHADFCMVTSLHDGMNLVAKEFISVRDDDDGALILSQFTGASSELRDAILV
                     NPYDVDGMADAIRSAVAMAPDERRARMARMRQAVREHNIYRWAGLLLSELERIPEGSA
                     NHVTAGASSVS"
     gene            complement(4234..4869)
                     /locus_tag="VNU03_07835"
     CDS             complement(4234..4869)
                     /locus_tag="VNU03_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWP74409.1"
                     /translation="MRQMMKIGVLMPAAVLLVSGCATKDWVREQFTKSDAIVDQRFVK
                     VEGQVDGVSTEVKGVTTQVKSLEGAVAEAKGRADAAMTKAEGVDTRLTRLWSNRYNQK
                     TADTLEVYFGFDKAELTDGAQTALAGVVKELQGNPTLIVELGGYTDPKGTVDYNYGLS
                     QRRVEAVRRFLVEKGVQLSRVHAVGLGPIAAKESPDEKKRRVTVKMLVDQD"
     gene            complement(5109..>6310)
                     /gene="hpnR"
                     /locus_tag="VNU03_07840"
     CDS             complement(5109..>6310)
                     /gene="hpnR"
                     /locus_tag="VNU03_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HWP74410.1"
                     /translation="FVGGHSASFVAAEFLEHAEGAIDCIVRGEGEVIAPRVLEAIGDP
                     KLDTLPGVVTLTGAGPSPTLLDNLDLHLPARDLGRKRHKYFIGVLDPCASVEFTRGCP
                     WDCSFCSAWTFYGRSYRKGTPEAAGEDLARIREPNVFIVDDVAFIHAEHGDAIANEIE
                     RRRIRKQYYLETRCDVLIKNQELFARWKRLGLFYMFLGIEALDEEALKLHRKRVTLNE
                     NLRALEIARSLGLTVAINIIADPSWDEARFAAVRDWALTVPEVVHLTVATPYPGTELW
                     LTESRQLTSRDYRLFDVQHAVLPTKLPLKRFYELLVNTQSVLNRKHLGWGALKLYGGA
                     ALRAASRGQTNYLRMLWKFSSVYNTERQYADHLKPVRYEMKPPRPLLGSKPSKQELYI
                     HMPAPVH"
BASE COUNT          940 a         2156 c         2230 g          984 t
ORIGIN      
        1 cctcgcgact ggtgcgagag cagcgagaga actacttgac gatccagagc ttcctggcga
       61 aggcccgaga ggccctctcg acccgcgacg tgcagcgtgc gttcaccctg gccgacaagg
      121 cctacctgct cgccgacgag ctctcgaagg gcccgcgctg aagggcgtgt aaggcttacc
      181 cggctcggta agctttaccg accgggcccg agccccgcca tcgctcgacc acccctaagc
      241 ctgtgagtct gcggcctctt ttggccgcgc tggtatggca cgggggttgc gaagttaccc
      301 gccgggaatt cgacatctgc cggagaggag aacgtatgcc cagcggactg ctcggcggag
      361 cacagcggtg cctggccctc gcgctcgccg tgagcggcct cagcgtggcc tgcacgatcg
      421 accctccggc ggggaccccg ccaccgccga tgtcgggcgt cctcgagcgc ggcccgcggg
      481 agaccgcctt cggccgcacc ggcgacgagg tcgtcaacaa cccgcagctc cgtgacaaga
      541 tccgcagcat gttcggcacg gactgggcgc cgggcggcaa gctcgtgcgg ggcgcgatgc
      601 cgtacctcga gcgcacggag gcgccccgcg gcgtgcggat cggcgaccgg aactacgtcg
      661 cggtcaccgg ctgcgtgccg gccacctgcc ggacggaccg cgtggtgctc ctgatccagg
      721 agggcggcga cgccctgctc gcgcggctcg acgagggtgg cttctcgcac tactacgcct
      781 acggtggcga ctggggcctc gcgggctcga ctgcccgcat cgtggacagc gggctccgcg
      841 ccctcgagcg cggcggggac ccgtatcccc cgtacccgcg gtcctgaggc cggccgaccc
      901 ttgacccgcc ccggcccgct cgctatgctg acgtctgccc ttcggacccg ctggtactcg
      961 caaaaaagat gcgccttgcc gatatcgagc gcgcgacgtg ttcaggcggc accgacggcg
     1021 tccccttgcc gggagcgcgg cgcatgagtg accttgcccg catcctggga tgggtcggcg
     1081 actgggtcgc cggcgacgga aggctgctgc tcatgaccga ctacgacggg accctgacgc
     1141 cgatcgtgga cgatcccgcc gacgcgatcc tggccgagga gacccgcgag cagctcctgc
     1201 ggatcgcccg ctctgcccgc ggctccgtgg cggtcatctc ggggcgggga ctcgaagatc
     1261 tgaagacgcg tgtgtccatc ccggaggtgg tctacgccgg ctgccacggg ctcgagatcg
     1321 aggggccggg actcgcattc cgccatcccg acgcgctcgc ccagcagccg atgctgtggg
     1381 cggtgggcga ggagctgttg cggcgggccc cgacggtcgc cggcatgcgg atcgaggcca
     1441 agggcctggc ggtggccgtc cactaccgcc acgtggcccc ggacgagatc cggcgcgtcg
     1501 agatcgagct ggcccgggcc atccgcgagc agggaagctg cctcaagatc ttccacggga
     1561 ccaaggtgat cgaggtgctg ccccaggtcg cgtggaccaa gggcgagtgt gcgcggtgga
     1621 tccaggagca cgtgctgccg gccgggtccg ggccggccct ctggctctac ctgggcgacg
     1681 actggacgga cgagcatgcc ttcgaggtgc tgaagggcct ggccctcacg atccgggtgg
     1741 gcgatacggt acccgcctcc aaggcggcct accggctgcg cgacgtgggc gaggtgcggg
     1801 agctgctggg cgcgctcgcc gagggcgcga cggcggggag tcgggtatga agttccagct
     1861 ccggctggtg gcggggttgt ggctcggctc gctcctcgtg gtcggagcgt tcgcgtactt
     1921 ccagttcgtc gaggatcggc agcgcctgac cggggagctc gaccggcggg cggccctggt
     1981 gagcgaggga ctcaaggagg tgctcgaccc cgcgctcggc ccgcgggtga gcaagccgca
     2041 ggtcgaccgg ctgatcaaga agttcagcaa gccggatctg ggcctggcgg tctacgaccg
     2101 cgtggcgtcc ctgatcgcgg cgaccccgga cgtgaccaag cagctcgaga acccgccgcc
     2161 cgaagcgacc tgggcgctca cctcgggggc ggtgcagacc ggcttccgca aggtcggcgg
     2221 cacgatgcgc tacgtctacg ccgatcccat cctgcgcgag gacaagccgt ccggcgcgct
     2281 ggtgatcttc ctcgacgcaa gcgatctcca gaccgcggag tgggagctgt ggcgcctgaa
     2341 cgccatccgg ttcgtggtgc tcgcggtcgc cctcgccctc atcgccctgc tcgtcgtccg
     2401 catgagcctg acccagccgc tcgccaagat ggcgcgctgg accaaggcgg tgcggcgcgg
     2461 ccacgcgatc gatccgcccg agctgccgga cgagagcctc ttcgggccca tcgcccggga
     2521 ggtcacggtg ctggccaaga acctccagcg ggcgcgcgcc gccgccgagg aggaggccgc
     2581 gctgcgcctg atcggccaga ccctgtggac cgaggagcgg ctgaagcagt tcgccaagat
     2641 gcggctcggc gagcggccgc tggtggtggt ctcgaaccgc gagccggtca gccacgtctg
     2701 gaaggagggc cagatccagg tggtgacgcc ggccagcggc ctggtcaccg cgatggatcc
     2761 ggtcatgcgg gcgtgcggag gcgtgtgggt ggcgcaggcc agcggcgacg cggatcgcga
     2821 gaccgcggac gcgcggggac gcctgcgcgt gcccccggac gatccgcgct tcaccctcaa
     2881 gcgggtctgg ctcacgcccg aggaggaggc gggctactac tacggcttct ccaacgaggg
     2941 cctgtggccg ctctgccaca tcgtgcacaa ccgtcccctc ttccgcccgg aggactgggc
     3001 gcagtaccgc gcggtcaacg agaagttcgc ggccgcggtg ctggaggaga tcgcgggcac
     3061 cgagtcgccg atggtgctga tccaggacta ccacttcgcg ctgctgccgg gcctcatcaa
     3121 gcgcgagcgg ccggacgcgc gcaccgcgat cttctggcac atcccgtggc cgaacttcga
     3181 ggccttctcg atctgcccgt ggcaggacga gctgttgctg gggatgctgg gggccgacat
     3241 catcggcttc cacacccagt actactgcaa caacttcctg gacacggtgg agcgcgcgat
     3301 cgaggcccgc atcgactggg agcgcttctc ggtcactcgc ggccagcacg tcacctcggt
     3361 gaaaccgttc ccgatcagcg tggccccggg cttcgtggac aatccgcccg ccacgtcacg
     3421 ccaggagctg ctcgcgagcc tcgatatcac cgccgagttc atcggggtcg gggtggagcg
     3481 gctcgactac accaagggcc tgcccgagcg cttccgcgcc ctgggccgct tcttcgagcg
     3541 cttcccggag taccgcgagc gcctcgtgtt cgtgcagctc gcggcgccca gccgcagcac
     3601 cattccccgc taccaggcgc tggaggcgga ggtggacgcg gcggtgcagg aggtcaacgg
     3661 cgcctaccag accaagcgct ggaagccgat tctctatctc aagcgccacc acgagcatcg
     3721 cgacatctgg cccttctacc gccacgccga cttctgcatg gtcacgtcgc tgcacgacgg
     3781 catgaacctg gtcgccaagg agttcatctc ggtgcgggac gacgacgacg gggcgctcat
     3841 cctgagccag ttcaccggcg cctcgtcgga gctgcgcgac gccatcctgg tgaacccgta
     3901 cgatgtcgac gggatggccg atgccatccg ctccgcggtc gcgatggcgc ccgacgagcg
     3961 ccgggcgcgc atggcgcgga tgcgccaggc ggtgcgggag cacaacatct accggtgggc
     4021 ggggctgctc ctgagcgagc tcgagcgcat tccggagggg tcggccaacc acgtcaccgc
     4081 cggcgccagc tcggtgtcct gagctcggcg tcccgagcct gggccgggag agaaacagca
     4141 agggggaggg acgtgagtcc ctcccccttc cttcactaaa gaccacccct caccctgccc
     4201 tctcccctga ggggagaggg agggaggcgg gttctagtcc tggtcgacca gcatcttgac
     4261 ggtcacccgg cgcttcttct cgtcggggct ctccttggcg gcgatcggac ccaggcccac
     4321 cgcgtggacg cgggacagct gcacgccctt ctccaccagg aagcggcgca ccgcctccac
     4381 ccgacgctgg ctgagcccgt agttgtagtc cacggtcccc ttcgggtcgg tatagccgcc
     4441 gagctccacg atcagggtcg ggttgccctg cagctccttc accacgccgg ccagcgccgt
     4501 ctgggcgccg tccgtcagct cggccttgtc gaagccgaag tagacctcga gggtgtcggc
     4561 cgtcttctgg ttgtagcggt tcgaccacag gcgggtcagg cgggtgtcca cgccctcggc
     4621 cttggtcatc gcggcatcgg cgcggccctt ggcctcggcc acggcgcctt ccaggctctt
     4681 cacctgggtg gtcacgccct tgacctcggt gctcaccccg tcgacctggc cctctacctt
     4741 cacgaagcgc tgatccacga tggcgtccga cttcgtgaac tgctcacgga cccagtcctt
     4801 ggtggcgcag ccggacacca gcagcacagc cgccggcatc agaacgccga tcttcatcat
     4861 ctgtcgcata gaaccgcctc ctttgtgaac gttggccaac ctatcagcac gaatgcgaac
     4921 tgcctgggag agcaacaagt aagccagatc cggtgcgcga ggatcgctcg gtcggcgcgg
     4981 ccactttggg taggtatggc ctctacttgg gcggcgcgtc acgtgggcta atcgaggctc
     5041 ggagcggccg accggccgct ccggcattat aaagattaca cggtgggtgt aaccgttaca
     5101 cgcccgtatt aatggacggg ggcgggcatg tggatgtaga gctcctgctt gctcggcttg
     5161 ctccccagga gcgggcgggg tggcttcatc tcgtagcgga ccggcttcag gtggtcggcg
     5221 tactgtcgct cggtgttgta gaccgagctg aacttccaga gcatacggag gtagttggtc
     5281 tggccccgcg aggcggcccg gagggcggcg ccgccgtaga gcttgagggc cccccagccc
     5341 aggtgcttgc ggttgaggac ggactgggtg ttcaccagca gctcgtagaa gcgcttgagg
     5401 ggcagcttgg tgggcaggac cgcgtgctgc acatcgaaga ggcgatagtc gcgcgaggtg
     5461 agctgccgcg actcggtcag ccagagctcg gtgccggggt agggggtggc caccgtgagg
     5521 tggacgacct cgggcacggt cagcgcccag tcgcgcaccg ccgcgaagcg cgcctcgtcc
     5581 cagctcgggt ccgcgatgat gttgatcgcc accgtgagcc cgagcgagcg ggcgatctcg
     5641 agcgcgcgca ggttctcgtt gagggtgacc cgcttgcggt gcagcttgag cgcctcctcg
     5701 tcgagggcct cgatgccgag gaacatgtag aagagcccga gccgcttcca gcgggcgaac
     5761 agctcctggt tcttgatcag cacgtcgcag cgggtctcga ggtagtactg cttgcggatg
     5821 cgccgccgct cgatctcgtt cgcgatcgcg tcgccgtgct cggcgtggat gaaggccacg
     5881 tcgtcgacga tgaacacgtt gggctcgcgg atgcgggcca ggtcctcgcc cgccgcttcc
     5941 ggggtgccct tgcggtagct gcgtccgtag aaggtccacg cgctgcagaa cgagcagtcc
     6001 cacggacagc cgcgggtgaa ctccaccgag gcgcaggggt cgaggacgcc gatgaagtac
     6061 ttgtggcgct tgcggcccag atcgcgcgcg ggcaggtgca ggtcgaggtt gtcgagcagg
     6121 gtcgggctcg gccccgcccc ggtcagcgtc accaccccgg ggagggtgtc gagcttcggg
     6181 tcgccgatgg cctccagcac gcgcggggcg atgacctcgc cctcgccacg cacgatacag
     6241 tcgatggcgc cctccgcgtg ctcgaggaac tcggcggcca cgaacgacgc gctgtggccg
     6301 ccgacgaaca
//
LOCUS       DATMCV010000336         6545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_618347, whole genome shotgun sequence.
ACCESSION   DATMCV010000336 DATMCV010000000
VERSION     DATMCV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618347"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VNU03_07845"
     CDS             complement(<1..712)
                     /locus_tag="VNU03_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74411.1"
                     /translation="MLNATRDLILPTTITGSYPRPHWFTEELRGRSFKDALGDSRFRE
                     QYLDAVACLVREQEMAGLDLVTDGDSRFDLTVGGKSWFYYVIERLRGITGAQDRSTGG
                     GWKALRPGHILYEVMEAYQPAVVGEPLARGPLQYSALFKTAQRLSAKPVKFGAITAQS
                     LAKMLVNQHYGGDRDVILALADIFNAELREVAAAGCRVIQVEEPQHHIAGAAGASDRD
                     LEFYTESVNREIAGVDTEI"
     gene            835..2067
                     /locus_tag="VNU03_07850"
     CDS             835..2067
                     /locus_tag="VNU03_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74412.1"
                     /translation="MHRRRFLMAMSGAMAAAAVRPARAADPIRIGYFGPLTGNFSQTG
                     KDMTDGFNLFWEEVGNKVAGREVKVIVEDSDPEPTGALTKVRRLVEQEKVHTVAGGLL
                     AATGYAIAPYIEQNKIPTLYPVTAPDDITQRKPVRWVVRTSASGSQATHPLGDYAYKQ
                     LKLRRVATISMDYAFGWETTAGFQRVFEELGGKVVQRIWTPLTIQDYAPYLASLKKDI
                     DGVYACHTGGLSPRFLKTWSDAGLKNIPLVGIGTLTDENVLKGMGDEALGVVTSLIYS
                     SALDTPANRKFSAAYEKRFGRGTSLYSAQGYTGARFYYEALKAINGEIENKEKFMAAL
                     RKVEIAEDPRGPMKMDDLGNPIENVYVRKVERVGGKLQNTVIHTYPNVSQFWTYKQDE
                     YLKTPAYDRNYPPCKFCD"
     gene            complement(2080..2298)
                     /locus_tag="VNU03_07855"
     CDS             complement(2080..2298)
                     /locus_tag="VNU03_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74413.1"
                     /translation="MIFRGAAAGRGAAAGALLMIALAACATGPPVPPAYTQEELAERC
                     VRTGGWWHGVEPDSLFSGFCEYQSPRFP"
     gene            complement(2295..3650)
                     /locus_tag="VNU03_07860"
     CDS             complement(2295..3650)
                     /locus_tag="VNU03_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP74414.1"
                     /translation="MAQSDPTAKALSRIDQDVVTKLAVDLVSIPSPTGSERELATYVA
                     DYMAAGGLEVTLQEVGPNRANAIGVLRGEGDGPRLMFNGHLDTSITGIEEEDYPMTGP
                     HGAASRTKGFVKDGHVLGCGAYNMKGGVAAMVGAALAIKAAGVRLRGDLVVAAVAGEI
                     EKAPVKGLLRTYAGEAYNGGGVGTRHLLSRGNITDYALVPEPTHMGVLRSRLGLLFIK
                     LTTRGDMVRACFADKGRTAMAKMFDVLSALERDFNPRIARYTQVQEDSVYTPGFSIGA
                     IEAGWPYKPWASPAVCCAYIDFRMPLGHTVLGAERELRGFLEELQARDPDLKVEMEIF
                     LSKASNTTPADSHVYTSCRQHFETLIGPYERCTHPLASYSDDTCIMREQAMEAVVFGP
                     GGLRYRGQEDSKGLGIGGEAVSIEDMLNVARVFTATAVDVCSRTRDVLKIRDQWSDAP
                     A"
     gene            3759..4517
                     /locus_tag="VNU03_07865"
     CDS             3759..4517
                     /locus_tag="VNU03_07865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWP74415.1"
                     /translation="MTPQRVAVRWIDGESQAAPFAYVARPASDPPIGGVLLLHEAFGL
                     NADIQTLAGKLAERGYAVLAPDLYRRGPDRLAGYDERAKAIAMLKTLPDGQVLDDTAR
                     GLDLLQRETGGAALGVVGLRIGGRYALLAAAREPGRIRAAVSYYGAGIDGGTISPLWT
                     LNAIEQVQALPTPMLLFFVGDDPTVPSAEVDSIDARLRRGGATYEIVRYPGVKPGFVF
                     PGRDTYREGEAEDAWNRQLGFLERNLGRGARRAP"
     gene            4581..5816
                     /locus_tag="VNU03_07870"
     CDS             4581..5816
                     /locus_tag="VNU03_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74416.1"
                     /translation="MRLIRPGLIVLCLLAAALAPVRPASAAESIRIGYLGPLTGIFAQ
                     AGKDMLDGLKLSLEQVGYQAGGRKIELIEEDTEGNSATAIAKYRKLVGHDKIHVLTGV
                     LLVNVGNSLVPLIEKDQLPTLFLTTPDDLTKRKIAKWILRSNFSASQIMHPLGDYAAK
                     TLKYKRVAVIAMDNGFGHEEAGGFQRVFEDAGGRVVQKIWVPLNVLDFAPYLSQVAKD
                     VDAVCAVFVAGQAVRFVKQYGEAGLKGKTPLIGTGVMIDESALRGMGDEAVGTIGTLL
                     WSPTLQTPANQTFMKLAEGKLGRTPAYFHAIMYSSGRWITEAANALNGQVEDREKLVA
                     AIRRSIETTPDPRGPIKLDEWGNPTENVYVLRVDQVGGKLATTVIHTYPAVSQFWTYK
                     PEEFLKSPPYSRDYPPVKP"
     gene            5829..>6545
                     /locus_tag="VNU03_07875"
     CDS             5829..>6545
                     /locus_tag="VNU03_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP74417.1"
                     /translation="MRVGFFFWPYTPEYTARMARLGEELGFDLVGIADTPGNALDPWV
                     AMTLAAAATSRVRLATCVTNLVTRHPLITASAAASVDAAAGGRTILGIGAGHSGVANV
                     GGVPTRAAELREGLAFLKTALAGAPASWRGAATHLAWVKRPVPVYAAASGPAALRAAG
                     AVADGAFVNYGLGAAEVARVRGLIAEGAAAAGRPASAVDVWSIACLDVADRREDALDK
                     LGNILGFVAAYILGPDPEGRG"
BASE COUNT         1015 a         2339 c         2234 g          957 t
ORIGIN      
        1 agatctcggt gtcgacgccc gcgatctccc ggttcaccga ctcggtgtag aactcgaggt
       61 cccggtcgga ggcccccgcc gctcccgcga tgtgatgctg cggctcctcg acctggatga
      121 cccggcagcc cgccgccgcg acctcccgga gctcggcgtt gaagatgtcg gccagggcca
      181 ggatcacgtc gcggtcgcca ccatagtgct ggttcacgag catcttcgcg agcgactggg
      241 cggtgatcgc cccgaacttc accggcttgg ccgagagccg ctgggcggtc ttgaagagcg
      301 ccgagtactg gagcgggccg cgcgcgagcg gctcccccac caccgcgggc tggtaggcct
      361 ccatcacctc gtagaggatg tgcccgggtc gcagcgcctt ccagccgccg ccggtcgagc
      421 ggtcctgggc gccggtgatg ccccggagtc gctcgatcac gtagtagaac cacgacttgc
      481 cgcccaccgt gaggtcgaat cgggagtcgc cgtcggtgac caggtcgagc ccggccatct
      541 cctgctcgcg caccaggcac gccaccgcgt ccaggtactg ctcgcggaag cgcgagtcgc
      601 cgagtgcgtc cttgaagctc cgcccgcgca gctcctcggt gaaccagtgc gggcgcgggt
      661 aggagccggt gatggtggtg ggcaggatca agtcccgggt cgcgttcagc atggcccgac
      721 tctgccgact gcgccgcggg ctgtcaagcc cgccgcggtt gacaaaccgc ggggcgctcc
      781 atagtctagc cgcccgggca cccgcaattc cctcgcggta gaggaggcca gtccatgcac
      841 aggcgacgat tcctcatggc catgagcggc gcgatggcgg ccgcggcggt tcgaccggcc
      901 cgggccgccg acccgattcg catcggctac ttcgggccgc tcaccggcaa cttctcccag
      961 accgggaagg acatgaccga cggcttcaac ctcttctggg aggaggtcgg caacaaggtc
     1021 gcgggccgcg aggtgaaggt catcgtggag gactcggatc ccgagcccac cggcgcgctg
     1081 acgaaggtgc ggcgcctggt cgagcaggag aaggtgcaca ccgtggccgg cgggctgctc
     1141 gcggccaccg gctatgcgat cgcgccctac atcgagcaga acaagatccc gacactctat
     1201 ccggtgaccg cgcccgacga catcacgcag cgcaagccgg tccgctgggt cgtgcgcacg
     1261 tcggcctcgg ggagccaggc gacccatccg ctgggcgact acgcgtacaa gcagctcaag
     1321 ctgcggcggg tggcgaccat cagcatggac tacgccttcg gctgggagac caccgccggc
     1381 ttccagcggg tgttcgagga gctggggggc aaggtggtgc agcgcatctg gacgccgctc
     1441 accatccagg actacgcgcc ctacctggcg tccctgaaga aggacatcga cggggtctac
     1501 gcctgccata ccggcggcct ctctccgcgc ttcctgaaga cctggagcga cgccggcctg
     1561 aagaacatcc cgctcgtcgg catcggcacc ctcaccgacg agaacgtgct caagggcatg
     1621 ggagacgagg cgctcggggt cgtcacctcg ctgatctaca gcagcgcgct cgacaccccg
     1681 gccaaccgga agttctccgc cgcctacgag aagcgcttcg gccgcggcac ctcgctctat
     1741 tccgcccagg gctacaccgg ggcgcgcttc tactacgagg cgctgaaggc catcaacggc
     1801 gagatcgaga acaaggagaa gttcatggcc gccctccgca aggtggagat cgcggaggac
     1861 ccgcgcgggc ccatgaagat ggacgacctc ggcaatccga tcgagaacgt ctacgtccgg
     1921 aaggtggagc gggtgggcgg caagctccag aacacggtga tccacaccta tccgaacgtc
     1981 tcgcagttct ggacctacaa gcaggacgaa tatctcaaga ccccggcgta cgatcgcaac
     2041 tatccgccgt gcaagttctg cgactagcga ggcacggcat caaggaaatc tcggggactg
     2101 gtactcgcag aacccgctga acagcgaatc cggctccacc ccgtgccacc atccgccggt
     2161 gcggacgcac cgctccgcga gctcctcctg cgtgtaggcc ggcgggaccg gcggcccggt
     2221 ggcgcacgcc gcgagcgcga tcatcagcag ggcgcccgcg gcggcgcctc gacccgcggc
     2281 ggcgcctcga aagatcacgc gggtgcgtcg gaccactggt cgcggatctt gagcacgtcc
     2341 cgcgtgcgcg agcacacgtc caccgcggtg gcggtgaaca cccgcgccac gttgagcatg
     2401 tcctcgatgc tcaccgcctc gcccccgatc ccgaggccct tgctgtcttc ctggccgcga
     2461 tagcggagcc cgccggggcc gaacaccacc gcttccatcg cctgctcccg catgatgcac
     2521 gtgtcgtcgc tgtagctggc gagcggatgg gtgcaccgct cgtacggccc gatgagggtc
     2581 tcgaagtgct ggcggcacga cgtgtagacg tgggagtcgg ccggcgtggt gttggaagcc
     2641 ttcgacagga agatctccat ctcgactttc agatctgggt cgcgagcctg gagctcctcg
     2701 aggaagccgc gcagctcgcg ctccgcgccc aggacggtgt ggccgagggg catgcggaaa
     2761 tcgatgtacg cgcagcagac cgccggggac gcccacggct tgtagggcca gcccgcctcg
     2821 atggccccga tcgagaagcc cggggtgtac accgagtcct cctgcacctg cgtgtagcgg
     2881 gcgatccgcg gattgaagtc gcgctccagc gcgctcagca cgtcgaacat cttggccatc
     2941 gcggtccggc ccttgtcggc gaagcaggcg cggaccatgt ccccgcgcgt cgtcagcttg
     3001 atgaacagga ggcccagccg gctgcgcagc acgcccatgt gggtcggctc cggcaccagc
     3061 gcgtagtccg tgatgttgcc ccggctaagc aggtggcggg tgcccacgcc gccgccgttg
     3121 taggcctcgc cggcgtaggt ccgcagcagg cccttcaccg gcgccttctc gatctcgccg
     3181 gcaaccgctg cgaccaccag gtcgccgcgc agccgcacgc cggccgcctt gatggccagc
     3241 gccgcgccga ccatcgccgc gaccccgccc ttcatgttgt acgctccgca gccgagcacg
     3301 tgcccgtcct tgacgaagcc cttggtccgg gacgcggcgc cgtgcggccc ggtcatcggg
     3361 tagtcctcct cctcgatccc ggtgatcgag gtatcgagat gcccattgaa catgagccgc
     3421 ggcccgtcgc cctcgccgcg gagcaccccg atcgcgttgg cgcggttcgg ccccacctcc
     3481 tgcagggtca cctcgagacc gcccgcggcc atgtagtcgg ccacgtaggt cgccagctcg
     3541 cgctccgatc cggtggggct cgggatgctg acgaggtcga ccgcgagctt ggtcaccacg
     3601 tcctggtcga tacggctgag ggctttcgcg gtcgggtcac tctgggccat ggagacctcg
     3661 cggttcgggt gaatgggctc ttgtatacta ctgacatact agaagacggg cgacacggta
     3721 ccccgctcgc ccgcacgttg tcaacgtgag gagccaggat gacgccgcag cgcgttgccg
     3781 tccgatggat cgatggggag tcccaggccg caccgttcgc ctacgttgcc cggccggcga
     3841 gcgacccacc catcggcggc gtgctgctcc tgcacgaggc gttcggtctg aacgccgaca
     3901 tccagaccct ggccgggaag ctcgcggagc gcgggtacgc ggtgctggcg ccggacctgt
     3961 accggcgcgg acctgatcgc ctcgcgggct acgacgagcg cgccaaggcc atcgcgatgc
     4021 tcaagacgct tcccgacggt caggtgctgg acgataccgc gcgcgggctt gacctcctgc
     4081 agcgcgagac cggcggcgcg gccctggggg tcgtgggcct gcggatcggg ggccgctacg
     4141 ccctgctggc cgcggcacgt gagccgggtc ggatccgggc cgcggtctcc tactacggcg
     4201 ccggaatcga cggcggcacc atctcgccgc tgtggaccct caacgcgatc gagcaggtcc
     4261 aggccctgcc cacgccgatg ctgctgttct tcgtcggcga cgatcccacc gtgccgtcgg
     4321 ccgaggtcga ttcgatcgac gcccgtctcc ggaggggcgg cgccacgtac gagatcgtcc
     4381 gctacccggg ggtgaagccc ggattcgtgt tccccggccg cgacacctat cgcgagggcg
     4441 aggccgagga tgcctggaat cggcagctag gatttctgga gcgcaatctg ggaagaggcg
     4501 cgcgccgcgc gccttgaccg ggtcgggagc gaccgagtag cctaggccgc cgtgaccttc
     4561 gcgccaaagg agccgcgccc atgcgactga tccgccccgg cctgatcgtg ctgtgcctgc
     4621 tcgctgccgc gctcgcgccg gtgaggccgg cgtcggccgc cgaatcgatc cgcatcggct
     4681 atctcggccc gctcaccggc atcttcgccc aggccggcaa ggacatgctc gacgggctca
     4741 agctgtcgct cgagcaggtc ggctaccagg cgggcgggcg caagatcgag ctgatcgagg
     4801 aagacaccga gggcaactcg gccaccgcca tcgccaagta ccgcaagctc gtcggccacg
     4861 acaagatcca cgtgctcacc ggcgtgctgc tggtcaacgt gggcaacagc ctggtgccgc
     4921 tcatcgagaa ggaccagctc ccgaccctgt tcctcaccac cccggacgac ctcaccaagc
     4981 gcaagatcgc caagtggatc ctccgctcca acttctccgc gagccagatc atgcacccgc
     5041 tcggcgacta cgcggcgaag acgttgaagt acaagcgggt ggcggtcatc gcgatggaca
     5101 acggcttcgg ccacgaggag gcgggcggct tccagcgggt gttcgaggac gcggggggcc
     5161 gggtggtgca gaagatctgg gtgccgctca acgtgctcga cttcgcgccc tacctctcgc
     5221 aggtggccaa ggatgtcgac gcggtctgcg cggtcttcgt ggccggccag gcggtgcgct
     5281 tcgtgaagca gtacggcgag gccgggctca agggcaagac cccgctcatc ggcaccgggg
     5341 tcatgatcga cgagagcgcg ctgcgcggca tgggggacga ggcggtcggc accatcggca
     5401 cgctcctctg gagccccacg ctgcagacgc cggccaacca gaccttcatg aagctggccg
     5461 agggcaagct cgggcgcacc cccgcctact tccacgcgat catgtacagc agcgggcgct
     5521 ggatcaccga ggccgccaac gccctcaacg gccaggtcga ggaccgcgag aagctggtgg
     5581 ccgcgatccg ccggtcgatc gagaccaccc cggacccgcg cgggcccatc aagctcgacg
     5641 agtggggcaa cccgaccgag aacgtctacg tgctgcgggt ggaccaggtc ggcggcaagc
     5701 tcgccaccac cgtgatccac acctacccgg cggtctcgca gttctggacc tacaagccgg
     5761 aggagttcct caagagcccg ccctactcgc gcgactatcc acctgtgaag ccctgagcca
     5821 ggcgcccgat gcgcgtcggc ttcttcttct ggccgtacac gccggagtac accgcgcgca
     5881 tggctcgcct cggcgaggag ctgggcttcg atctcgtcgg catcgccgac accccgggca
     5941 acgcgctcga cccgtgggtc gccatgaccc tcgcggccgc cgcgacctcg cgcgttcggc
     6001 tcgcgacctg cgtgaccaac ctggtcacgc gccacccgtt gatcaccgcc tccgccgccg
     6061 cctccgttga cgcggccgcg ggcgggcgga ccatcctggg catcggcgcc gggcacagcg
     6121 gcgtcgccaa cgtgggcggc gtgcccaccc gcgcggccga gctgcgcgag ggcctcgcgt
     6181 tcctcaagac cgcgctcgcc ggcgcgcccg cctcgtggcg cggggcggcc actcacctgg
     6241 cctgggtcaa gcgcccggtg ccggtctacg ccgccgcgtc gggcccggcc gcgctccggg
     6301 cggcgggcgc cgtcgcggac ggtgcgttcg tgaactacgg gctcggcgcc gccgaggtgg
     6361 cccgggtccg cgggctgatc gcggaaggcg cggccgcggc gggccggccc gcgagcgcgg
     6421 tcgacgtctg gtcgatcgcc tgcctcgacg tggccgaccg gcgcgaggac gcgctcgaca
     6481 agctcggcaa catcctgggc ttcgtggccg cctacatcct ggggccggat cccgagggcc
     6541 gcggc
//
LOCUS       DATMCV010000337         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_152997, whole genome shotgun sequence.
ACCESSION   DATMCV010000337 DATMCV010000000
VERSION     DATMCV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152997"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1090
                     /gene="queG"
                     /locus_tag="VNU03_07880"
     CDS             158..1090
                     /gene="queG"
                     /locus_tag="VNU03_07880"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWP74418.1"
                     /translation="MSGGSLGALTLTGSVKALALELGFDLVAVGPAAPPEHGAALRRW
                     VAAGHAGTMGYLERRLEERLDPDRVLPGARAAVCVALNYYQGEPVDPSWRPIARYAWG
                     RDYHDVIGPRLERLATHLADAAGARSRGYVDTGPVLERDLAARAGLGWIGKNTMLLHP
                     ALGSWFFIGVLLTTAELVSDAPLADRCGSCRACLDACPTGAFLAPYVLDSRRCISYLT
                     IEHRGDIDPDVHPDMTGWQFGCDVCQDVCPWNRKAPPTPHPEFRPRAAYPGAEAISHM
                     DDASICDAFRDTALLRAKPAGLRRNALIYLENDS"
     gene            1206..1754
                     /gene="def"
                     /locus_tag="VNU03_07885"
     CDS             1206..1754
                     /gene="def"
                     /locus_tag="VNU03_07885"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWP74419.1"
                     /translation="MAILKVARLGHPVLRQLAASVPMGEIRSAGTQRLIDDMIETMRE
                     YNGAGLAAPQVHELQQICVIEVHGNPRYPDAPAIPLTVLINSVVTPLTEEMEDGWEGC
                     LSVPDMRGVVPRFGSVRLEAFDREGGRIDVVAKEFFARVIQHETDHLHGRVYLDRMRD
                     FTTLSHLAEWNRYWLGNQEQDD"
     gene            1766..>2078
                     /locus_tag="VNU03_07890"
     CDS             1766..>2078
                     /locus_tag="VNU03_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74420.1"
                     /translation="MKVVAVVRDLFFVARIRETARLVGTPLVFARTPQEIAAQLAPEP
                     PALVPALVIIDLTTNGWDYAAIFDALAAAPDVPVLGFTTHALARETQPLHARCRRVVT
                     KE"
BASE COUNT          278 a          780 c          714 g          306 t
ORIGIN      
        1 tgcggcgctt cgcatccgtc gaggagggcc tggccgccgc ggtcggcctg gaggaccggt
       61 ggcgaaccga cggccgctgg ttccgctaca tcgccttcct cgagtccggc ggccgcacgc
      121 tcacccgcag ccacctgctc gccaaggcgc ggcgcgcgtg agcggcggga gcctgggggc
      181 gctgacgctc accgggtcgg tgaaggccct ggcgctggag ctggggttcg acctcgtcgc
      241 ggtgggcccc gccgcaccgc ccgagcacgg cgcggcgctc aggcgctggg tcgcggccgg
      301 ccacgccggc accatgggct acctcgagcg acggctcgag gagcggctcg atccggaccg
      361 ggtgctgccg ggtgcgcgcg cagccgtgtg cgtggcgctc aattactacc agggcgagcc
      421 cgtcgatccc tcctggcgcc cgatcgcgcg ctacgcctgg ggccgtgact atcacgacgt
      481 gatcgggccg cggctcgagc gcctggccac tcatctggcc gacgccgccg gcgcgcggag
      541 ccgcgggtac gtggacacgg ggccggtgct cgagcgcgat ctcgcggcgc gggccgggct
      601 cggctggatc gggaagaaca ccatgctgct gcacccggcg ctcggctcgt ggttcttcat
      661 cggggtgctc ctgaccaccg cggagctcgt ctccgacgcc ccgctggccg accgctgcgg
      721 ctcctgtcgc gcctgcctcg acgcctgtcc gaccggggcc ttcctcgccc cctacgtgct
      781 cgactcgcgc cgctgcatct cgtatctgac gatcgagcac cggggcgaca tcgaccccga
      841 cgtgcacccc gacatgaccg gctggcagtt cggctgcgac gtctgccagg acgtgtgccc
      901 gtggaaccgg aaggcgccgc cgacgccgca tcccgagttc cgtccccggg cggcatatcc
      961 cggggcggag gcgatctcgc acatggacga cgcctcgatc tgcgacgcgt tccgcgacac
     1021 tgcgctcctt cgagccaagc ctgcgggact ccgccgcaat gccctgatat atctggagaa
     1081 cgactcgtga atccggcggt cgtgttccgg ggacccaccc tcaacgccgc gcgacggcgc
     1141 agcgtgctgg ccggcgtcga gccgtcgcga ggccctgtgc tacaattcga ccgcgagggc
     1201 accccatggc catcctgaaa gtcgcacgtc tcgggcaccc ggtacttcgc cagctcgccg
     1261 cttccgtccc gatgggggag atccgctccg ccgggaccca gcgcctgatc gacgacatga
     1321 tcgagaccat gcgcgagtac aacggggccg ggctcgccgc tccccaggtg cacgagctcc
     1381 agcagatctg cgtgatcgag gtgcacggca acccgcgcta ccccgacgcc cccgcgattc
     1441 cgctcaccgt gctgatcaat tcggtggtga ccccgctgac cgaggagatg gaggacggct
     1501 gggagggctg cctctcggtg cccgacatgc ggggcgtggt cccgcgcttc ggcagcgtgc
     1561 gtctcgaggc gttcgaccgc gagggcggcc ggatcgacgt ggtggccaag gagttcttcg
     1621 cccgcgtcat ccagcacgag accgaccacc tgcacggccg ggtctacctc gaccggatgc
     1681 gggacttcac caccctctcg catctggccg agtggaaccg ctactggctc ggcaaccagg
     1741 agcaggacga ctaggctcgc gacggatgaa ggtcgtcgcc gtcgtccgcg atctcttctt
     1801 cgtggcccgc atccgggaga ccgcgcgcct ggtgggcacc ccgctggtct tcgcccgcac
     1861 cccgcaggag atcgcggccc agctcgcgcc ggagccgccc gcgctggtgc ccgcactcgt
     1921 gatcatcgat ctcaccacca acggctggga ctacgccgcc atcttcgacg ccctcgcggc
     1981 cgcccccgac gtgccggtgc tgggcttcac gacccacgcg ctggctcgcg agacccagcc
     2041 gctgcacgcc cgctgccggc gggtggtgac caaggaga
//
LOCUS       DATMCV010000338         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_420277, whole genome shotgun sequence.
ACCESSION   DATMCV010000338 DATMCV010000000
VERSION     DATMCV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420277"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VNU03_07895"
     CDS             complement(<1..183)
                     /locus_tag="VNU03_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74421.1"
                     /translation="MSRAKARAKPKKAGAKPKKAPPKKDAVRAEVRALVHGPSYMTFD
                     DVVADFPIEHINTRPPN"
     gene            complement(180..485)
                     /locus_tag="VNU03_07900"
     CDS             complement(180..485)
                     /locus_tag="VNU03_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74422.1"
                     /translation="MKAQGTVKGILVEGGDGQEYLLGFRPGSPGPTPTLSRRRADGRF
                     EPVEDVQEASRLIVKQVGISGPHLIGPGWGPHVFLWSAFKEALKALPTVPSWPTWRP"
     gene            580..2046
                     /locus_tag="VNU03_07905"
     CDS             580..2046
                     /locus_tag="VNU03_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP74423.1"
                     /translation="MAATRNRYAFRLSPGMRRGFEAETPIPTRLVSNEEFPPLPQTPA
                     QRQVEERILAEAARLAPRLGLSRRDFLRTSGGVATSLLAMNAVFGHFWDVRPVEAADP
                     AAFKERSGDPYFIFDVQLHYVGAGYDPRDGETGRRGAVSKQALLGLRQGSKRLNPKLA
                     ADRGTLADLAWANMVKEVFLDSETDIGLISTPPGPYPQEAVVPPKEMTHIRDEINRVT
                     QSQRMLAHGLITPQLGQVDLDFMDQQAAMLKVDAWKGYTGAAPKGFDRGWFVDDEKIA
                     YPMLERARKLGIKRVCLHKGLPLGPVSDYNHPRDVIKAARDFPDIDFVLYHAGLLTVP
                     RTQSADVPWTTEFCRMKKKQLGLRNIYMELGSTFGQLATTNPTACAHLMGQIVDAFGA
                     DHVLWGTDSIWYGTPQWQIEAFRRFEIPQALVDAHGYQPLNRLVKEQIFGLNAARLFG
                     VDVRAKRNEVPKDYLSQIKMAYLDEGPTPSHRFYGWIA"
BASE COUNT          335 a          774 c          711 g          316 t
ORIGIN      
        1 gttgggaggg cgcgtgttga tgtgctcgat cgggaagtcg gccaccacgt cgtcgaaggt
       61 catgtacgac ggcccgtgga ccagggcgcg gacctccgcg cgcaccgcgt ccttcttcgg
      121 aggggccttc ttcggcttcg cgccggcctt cttcggcttc gcccgggcct ttgcgcgact
      181 catggccgcc acgtgggcca gctcggcacg gtgggaagcg ccttcagcgc ctccttgaag
      241 gcgctccaca ggaagacgtg cggcccccat cccggcccga tcaggtgcgg gcccgagatg
      301 ccgacctgct tcacgatcaa gcgggacgcc tcctgcacgt cctccaccgg ctcgaagcgg
      361 ccgtcggcgc gacggcggct cagcgtgggc gtcgggcccg gcgagcccgg ccggaagccc
      421 agcaggtact cctggccgtc gccgccctcc accagaatgc ccttcacggt accctgtgcc
      481 ttcatggcgc gctcctcccg agcccaaggt acgcgcggcg gcgcatcatt gacaaggccg
      541 cctccgggtc tagactcggt ggccaggagg acgacgatca tggccgcgac ccgcaatcgt
      601 tacgcgttcc gactctcccc cggcatgcgg cggggcttcg aggccgagac gccgatcccc
      661 acccgcctcg tctccaacga ggagttcccg cccctgccgc agacgcccgc gcagcggcag
      721 gtcgaggagc gcatcctcgc cgaggccgcg cggctcgccc cgcggctcgg cctgagccgc
      781 cgcgacttcc tgcgcacgag cggcggggtg gcgacctcgc tgctggcgat gaacgcggtc
      841 ttcgggcact tctgggatgt gcgcccggtc gaggccgccg accccgccgc cttcaaggag
      901 cggagcggcg atccctactt catcttcgac gtgcagctcc actacgtggg tgcgggctac
      961 gatccccgcg acggggagac gggccgacgg ggcgcggtga gcaagcaggc cctcctcggg
     1021 ctgcggcagg gctcgaagcg cctgaacccg aagctggccg ccgaccgtgg caccctggcg
     1081 gacctggcgt gggccaacat ggtcaaggag gtcttcctcg acagcgagac cgacatcggg
     1141 ctcatctcca cgcccccggg cccgtatccg caagaggccg tggtgcctcc gaaagagatg
     1201 acgcacatcc gcgacgagat caaccgggtc acccagtccc agcggatgct cgcccacggg
     1261 ctcatcaccc cgcagctcgg ccaggtcgac ctcgatttca tggaccagca ggccgcgatg
     1321 ctcaaggtgg acgcgtggaa gggctacacc ggcgccgcgc ccaagggctt cgaccgcggg
     1381 tggttcgtgg acgacgagaa gatcgcgtac ccgatgctgg agcgtgcgcg caagctcggg
     1441 atcaagcggg tgtgcctgca caagggcctg ccactcggcc cggtctccga ctacaaccat
     1501 ccgcgcgacg tcatcaaggc ggcgcgcgat tttcccgaca tcgacttcgt gctctaccac
     1561 gccgggctgc tcaccgtgcc tcgcacgcaa tcggcggacg tgccgtggac caccgagttc
     1621 tgccggatga agaagaagca gctggggctc cggaacatct acatggagct cgggtcgacc
     1681 ttcggccagc tcgccaccac caaccccacc gcgtgcgcgc acctcatggg ccagatcgtc
     1741 gacgccttcg gcgccgacca tgtgctgtgg ggcaccgact cgatctggta cggcaccccg
     1801 cagtggcaga tcgaggcctt ccgccgcttc gagatcccgc aggccctggt cgatgcccac
     1861 ggctatcagc ccctcaaccg cctcgtgaag gagcagatct tcggcctcaa cgcggcccgc
     1921 ctcttcggcg tggacgtgcg ggccaagcgc aacgaggtgc ccaaggacta cctgagccag
     1981 atcaagatgg cctacctcga cgagggcccg acgccgagcc accgcttcta cggctggatc
     2041 gcctgagccc ctaccctctc cccggagggg agagggtagg gtgaggggcg ctaagggtga
     2101 ggggcgctag acgtacacgc gcgggatccg cgtgtt
//
LOCUS       DATMCV010000339         6699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_16557, whole genome shotgun sequence.
ACCESSION   DATMCV010000339 DATMCV010000000
VERSION     DATMCV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16557"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VNU03_07910"
     CDS             <1..521
                     /locus_tag="VNU03_07910"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWP74424.1"
                     /translation="KPRGYVTTRSDPDGRPVVIDLLPPAAAAVVYPVGRLDFDAEGLL
                     LLTNDGELANRLLHPRYEIPRVYEVEVEGHVAEADLPRWRRGVALDDGPAVPRAVTRL
                     AARGHTSRLRVTFAEGRYREVKRYCKALGHPVRRLKRVQFGPLRLGGLAPGGHRALTR
                     AELAALNDLRGG"
     gene            575..1636
                     /gene="pheA"
                     /locus_tag="VNU03_07915"
     CDS             575..1636
                     /gene="pheA"
                     /locus_tag="VNU03_07915"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWP74425.1"
                     /translation="MNLDDWRSRINNLDDEILKLLNQRGIAALRIGELKRQQDLPYFS
                     PEREAQVLDRLVALNDGPLGADAIRVIWREILSASLALEHPLPIAYLGPPGTFTHQAA
                     TRRFGSSAQLLPVHTIAEIFDEVERGRAEYGVVPVENSTEGAVNVTLDRLIDSDVTIT
                     GEMTLDISQHLISRATDLAGVKVVCSHPQGLAQCRAWLAAHLPEATTEETASTSGAVE
                     RARDDATVAAIASDMAAAVHGVPILRPRIEDNPSNSTRFLVIGRTTVPPTGRDKTSIL
                     FSMKNEPGVLYSILQPFATRGLNLTKIESRPTKRRPWEYVNFLDFEGHCETANVRDVL
                     DEVRERCQFLKVLGSYPAV"
     gene            1648..2763
                     /gene="hisC"
                     /locus_tag="VNU03_07920"
     CDS             1648..2763
                     /gene="hisC"
                     /locus_tag="VNU03_07920"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWP74426.1"
                     /translation="MSVSWESLANDHILGIAPYEPGKPIEELERELGISDVIKLASNE
                     NPLPPSDRVMKAMHTALTHINRYPDGSGHYLRQAIARKQGLTSDHIILGNGSNDLIEL
                     MVRAFLRSGEEAVIPHPSFVVYPMIVQAVGGIRVVVTLKDQRLDLDAMARAITPMTKM
                     VFIANPNNPTATIVTADEVEHFMDRVPDKVIVVFDEAYIEFAQGPDFPDALAYLKQGR
                     KVAVLRTFSKAASLAGLRVGYAAADPDCIALLNRIRQPFNVNSVAQAAALAALEDDSH
                     VRECLSMIDAGRHYLVQEFSAMGLKYAPSRANFILVDAGRSASEVFHWLLKEGVIVRP
                     MTSFGMESALRVTIGTPEENRRFVKALKKVLAGAKSA"
     gene            2760..3608
                     /locus_tag="VNU03_07925"
     CDS             2760..3608
                     /locus_tag="VNU03_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HWP74427.1"
                     /translation="MIRRLALVGLGLLGGSVAKAARALGLADEIVAVGRDRERLEPAR
                     RDGVVDRVTTDLAEGVGDADFCLLATPVATLAALLPAVWRALPSDAVLTDVGSTKATI
                     VHAAERLGEQRPLAFVGSHPMAGSEQAGYRVSRQDLFRGATVILTPTERTDSHAVKRV
                     GEFWEALGGRLVTLDPLTHDRATAAISHLPHLVADALVDAVVRMDPRFFEVAGRGFKD
                     TTRIAASDARMWREIFQENRAGLAEALTAFRAALDHLEGLLATGDVPAIEGALDRIRR
                     VREGVA"
     gene            3667..4383
                     /gene="cmk"
                     /locus_tag="VNU03_07930"
     CDS             3667..4383
                     /gene="cmk"
                     /locus_tag="VNU03_07930"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWP74428.1"
                     /translation="MRPAAGGDLVKRSGEQRDIVITIDGPAGAGKSTVAAALARRLGF
                     TLIDTGALYRGLAWATREAAVPLEEGPALRGLLDRTSVRLDGGRVLVDDRDVTVDIRT
                     PAISQLTSRLTALASVRDKMTPIQRSLAAPGAAVLEGRDTGSVVWPEAEVKFYLDADL
                     DTRARRRHDELAARGLDTALETVRDDIARRDRQDMERALAPLVKPEGAVVVDTTGLGI
                     AEVVDTLLRVVEPARCCTRS"
     gene            4365..5021
                     /locus_tag="VNU03_07935"
     CDS             4365..5021
                     /locus_tag="VNU03_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWP74429.1"
                     /translation="MLYAILKPIAVALMRLLFRLEVVSAGLVPATGPVLIVSNHVSLL
                     DPPLVGGAAPRPLFFMAKEELFRIPLFGRLIRSLNARPVRRDGSDTRALKAALAQLEE
                     GHALLVFPEGTRGEEGQPLRDGKPGVGMLAVLSGAPVVPVYVSGSGAALPRGRTLPRP
                     ARVRVTFGSPLTFKRASKSEGPREGKRAGDEGRKEAYREAAQEMMRAIGQLRDQQVKS
                     "
     gene            5093..>6699
                     /locus_tag="VNU03_07940"
     CDS             5093..>6699
                     /locus_tag="VNU03_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWP74430.1"
                     /translation="METENRKSKTLVGVSKEVDATEESMEYWFNRGVSDVEEGEVVRG
                     RVVEVRDSEILVDIGYKSEGTIPIEEFRHAGSLPKVGEEIEVYLESKEDNEGLIVLSK
                     DKADKIKVWDQISKAHDSGTPVEGRVAEVVKGGLAVDVGVRAFLPGSQVDLRPVKNLA
                     AMVGQTIRAKVIKLNRRRGNVVLSRRAVLEEEREEKKKHTLSVLAEGMVLTGTVKNLT
                     DYGAFIDLGGIDGLLHVTDMSWGRVGHPSEIFQIGDQVEVVVLHFDRETGRVSLGYKQ
                     KSSDPWANVDDRYPVGAKVSGRIVSLTNYGAFVELEPGVEGLVHVSEMSWTRRVRHPS
                     KLVNVGDPVEVMVLEVNKATKRISLGMKQVESDPWATITERYQPGNRVQGKVRNLTDF
                     GAFVELEPGVDGLLHISDMSWTRNIGHPSELLKKGQPIDTQILNVDRDNKRISLGLKQ
                     IQPDPWDSVASRYPMGSRVTGKVVRLTDFGAFVELEPGVDGLLHISQMSNRPIATPAD
                     ILNVGDELTLMVIRVDPNERRIGLSLK"
BASE COUNT         1051 a         2326 c         2390 g          932 t
ORIGIN      
        1 acaagccgcg cggctacgtg acgacccgga gcgatcccga cggccggccg gtggtgatcg
       61 acctgctgcc gcccgccgcc gcggcggtgg tctatccggt ggggcggctc gacttcgacg
      121 cggaggggct cctcctgctc accaacgacg gcgagctcgc caaccgcctc ctccacccgc
      181 gctacgagat cccgcgcgtg tacgaggtgg aggtggaggg gcacgtggcg gaggcggatc
      241 tcccgcgctg gcgccgcggc gtcgccctgg acgacggccc cgcggtgccc cgggcggtga
      301 cgcggctcgc cgcccgggga catacgagcc gtctgcgggt caccttcgcg gaggggcgat
      361 accgcgaggt caagcgctac tgcaaggcgc tcgggcaccc ggtgcgccgc ctcaagcgcg
      421 tgcagttcgg gccgcttcgc ctgggggggc tcgcgccggg cgggcaccgg gccctgaccc
      481 gggccgagct cgcggccctg aatgacttgc gtggggggta gcgatctcct atactcgtgc
      541 gggttgcgtg accgacgtgc ttcaggagcc cccgatgaac ctggacgatt ggcgatccag
      601 gatcaataac ctcgacgacg aaatcctcaa actcctcaac cagcgcggta tcgccgccct
      661 ccggatcggc gagctcaaga ggcagcagga tctcccgtac ttcagccccg agcgcgaggc
      721 ccaggtgctc gaccgccttg tcgcgctcaa cgacggaccg ctcggcgccg acgccatccg
      781 ggtgatctgg cgcgagatcc tgtccgcctc cctcgccctc gagcacccgc tgcccatcgc
      841 ctacctgggc ccgcccggca ccttcacgca ccaggcggcc acgcgccgct tcggatcctc
      901 cgcccagctg ctgccggtac acaccatcgc cgagatcttc gacgaggtgg agcgcgggcg
      961 cgccgagtac ggggtcgtgc cggtggagaa ctccaccgag ggggcggtca acgtgaccct
     1021 cgaccgcctg atcgactccg acgtgacgat caccggcgag atgaccctcg acatctcgca
     1081 gcacctgatc tcgcgcgcca ccgatctcgc gggggtgaag gtggtctgct cgcacccgca
     1141 gggcctcgcc cagtgccggg cctggctcgc cgcgcacctg ccggaggcga cgaccgagga
     1201 gaccgcgtcg acctcggggg cggtggagcg ggcgcgcgac gacgccacgg tggccgccat
     1261 cgcttccgat atggccgcgg ccgtgcacgg ggtgcccatc ctgcgacccc gcatcgagga
     1321 caacccgtcc aactcgaccc gcttcctcgt catcgggcgg acgacggtgc cgcccaccgg
     1381 gcgggacaag acgtcgatcc tcttctcgat gaagaacgag cccggggtgc tctacagcat
     1441 cctccagccc ttcgccacgc gcgggctgaa cctcaccaag atcgaatcgc gccccaccaa
     1501 gcggcgcccc tgggagtacg tgaactttct cgacttcgag ggtcactgcg agaccgccaa
     1561 cgtccgggac gtgctggacg aggtccggga gcgctgccag ttcctgaagg tcctcggctc
     1621 ctacccggcg gtgtgaagga gcaacgaatg agcgtgtcct gggaatccct cgccaacgat
     1681 cacatcctgg gcatcgcgcc ctacgagccc ggcaagccca tcgaggagct cgagcgcgag
     1741 ctcggcatca gcgacgtcat caagctcgcc tccaacgaga acccgctgcc tccgtcggac
     1801 cgggtcatga aggcgatgca caccgcgctc acccacatca accgctaccc cgacggcagc
     1861 gggcactatc tgcgccaggc catcgcccgg aagcagggcc tcacgagcga ccacatcatc
     1921 ctcggcaacg gctccaacga cctcatcgag ctgatggtgc gcgcgttcct gcgctccggc
     1981 gaggaggcgg tcatcccgca cccctcgttc gtcgtctacc cgatgatcgt gcaggcggtg
     2041 ggcgggatcc gggtggtggt gacgctgaag gaccagcgcc tcgacctgga tgcgatggct
     2101 cgcgcgatca ccccgatgac caagatggtg ttcatcgcga accccaacaa ccccaccgcg
     2161 accatcgtga ccgcggacga ggtcgagcac ttcatggacc gggtgcccga caaggtcatc
     2221 gtggtcttcg acgaggccta catcgagttc gcccagggcc cggatttccc cgacgcgctc
     2281 gcctacctca agcaggggcg gaaggtcgcg gtgctccgca ccttctcgaa ggcggcgagc
     2341 ctggccggcc tgcgggtggg ctacgcggcg gccgatccgg actgcatcgc gctgctcaac
     2401 cgcatccgcc agcccttcaa cgtcaactcg gtggcccagg ccgccgcgct cgccgccctg
     2461 gaggacgact cccacgtgcg cgagtgcctc agcatgatcg acgcgggccg ccactacctg
     2521 gtccaggagt tcagcgcgat ggggctcaag tacgccccgt cgcgcgccaa cttcatcctg
     2581 gtcgacgcgg ggcggagcgc gagcgaggtc ttccactggc tcctcaagga aggcgtcatc
     2641 gtgcgcccca tgacgagctt cgggatggag agcgcgctcc gcgtgacgat cggcacgccg
     2701 gaggagaacc gccggttcgt gaaggcgctc aagaaggtcc tggccggagc caagagcgcg
     2761 tgatccgtcg tctcgctctg gtcggtctcg ggctcctggg cgggtcggtg gcgaaggccg
     2821 cgcgcgccct gggcctggcc gacgagatcg tggcggtggg gcgcgaccgc gagcggctcg
     2881 agccggcccg gcgcgacggg gtggtggacc gcgtcaccac cgatctcgcg gagggagtgg
     2941 gcgacgccga cttctgcctc ctcgccaccc cggtcgcgac cctcgccgcg ctcctgcccg
     3001 cggtgtggcg cgcgctgccg tcggacgcgg tgctcaccga tgtgggcagc accaaggcca
     3061 ccatcgtgca cgcggcggag cgcctcggcg agcagcggcc gctcgccttc gtgggtagcc
     3121 atcccatggc cggctcggag caggccggct accgggtgtc gcgccaggat ctcttccgcg
     3181 gcgcgaccgt gatcctcacc cccaccgagc ggaccgactc gcacgcggtc aagcgggtgg
     3241 gggagttctg ggaagccctc ggcgggcgcc tcgtcacgct cgatccgctg acccacgacc
     3301 gggccaccgc cgccatcagc cacctgccgc acctggtggc cgacgcgctg gtggacgcgg
     3361 tggtgcgcat ggacccgcgc ttcttcgagg tggccgggcg cggcttcaag gacaccacgc
     3421 ggatcgcggc gtcggacgcc cgcatgtggc gcgagatctt ccaggagaac cgcgccggcc
     3481 tcgccgaggc gctcaccgcc ttccgcgccg ccctcgatca cctggaggga ctcctcgcga
     3541 ccggggacgt ccccgcgatc gagggggcgc tcgaccggat ccgccgcgtc cgcgaggggg
     3601 ttgcttgagc ctccgcgtca cccgcgtggc gcagcgcgtg ggcgagatcg cggggcccgg
     3661 cgaccggtgc gcccggcggc gggtggcgac ctcgtgaagc gcagcgggga gcagcgggac
     3721 atcgtgatca cgatcgacgg accggcgggc gcgggcaagt ccaccgtggc cgccgcgctc
     3781 gcccggcgcc tcggcttcac cctcatcgac accggggcgc tctaccgcgg gctcgcgtgg
     3841 gccacgcggg aggccgcggt gcccctcgag gagggtcccg cgctgcgcgg cctcctcgac
     3901 cggacgagcg tgcggctcga cggggggcgc gtgctcgtcg acgaccgcga cgtgacggtc
     3961 gacatccgca cccccgcgat cagccagctc acgtcgcggt tgacggcgct ggcctcggtg
     4021 agagataaga tgacgcccat ccagcgcagc ctggccgccc ccggggccgc cgtcctcgaa
     4081 ggacgcgaca ccggcagcgt ggtgtggccg gaggccgagg tgaagttcta cctcgacgcc
     4141 gacctcgaca cccgcgcccg acgccggcac gacgagctcg ccgcgcgcgg cctcgacacc
     4201 gcgctggaga cggtgcggga cgacatcgcg cgacgcgacc ggcaggacat ggagcgcgcg
     4261 ctggccccgc tggtgaagcc ggaaggcgcg gtggtggtgg acaccaccgg gctgggcatc
     4321 gcggaggtcg tggatacgct gctacgggtg gtggagccgg cgagatgctg tacgcgatcc
     4381 tgaagccgat cgcggtggcg ctgatgcgcc tcctgttccg gctcgaggtc gtgagcgcgg
     4441 ggctggtgcc cgccaccggc ccggtgctca tcgtgtccaa ccacgtgagc ctgctggacc
     4501 cgccgctggt cggcggcgcg gccccgcgcc cgctcttctt catggccaag gaagagctct
     4561 tccgcatccc gctcttcggt cggctcatcc ggagcctgaa cgcccggccg gtgcgccggg
     4621 acggctcgga cacgcgggcg ctcaaggcgg ccctcgcgca gctcgaggaa ggccacgccc
     4681 tgctggtgtt cccggagggc acgcggggcg aggaagggca gcccctgcgc gacggcaagc
     4741 ccggggtcgg catgctggcg gtcctgagcg gggcgccggt ggtgccggtg tacgtgtcgg
     4801 ggagcggggc ggcgctgcct cgcgggcgga cgctgccgcg gccggcccgg gtccgggtga
     4861 ccttcgggtc gccgctgacg ttcaagcgcg cgagcaagag cgaggggccc cgggagggca
     4921 agagagcggg tgacgaaggg cgtaaggagg cgtatcgtga agcggcccag gagatgatgc
     4981 gggcgatcgg tcagctccgg gaccagcagg tgaagtcctg aggcacggct gggatgccga
     5041 acgcggcaag agcgcgcggc cggcaaaata gattctaagg agaggtggag tcatggagac
     5101 cgaaaaccgg aagtcgaaga ccctcgtcgg cgtatcgaag gaggtggacg ccaccgagga
     5161 gagcatggag tactggttca accggggcgt gagcgacgtc gaggaaggtg aggtcgtacg
     5221 cggtcgcgtg gtcgaggtgc gcgacagcga gatcctcgtc gacatcggct acaagagcga
     5281 gggcacgatc ccgatcgagg agttccggca cgcgggcagc ctgcccaagg tcggcgagga
     5341 gatcgaggtc tacctcgagt ccaaggagga caacgagggg ctgatcgtcc tctccaagga
     5401 caaggccgac aagatcaagg tctgggacca gatctcgaag gcccacgaca gcggcacccc
     5461 ggtcgagggc cgagtcgccg aggtggtcaa gggcggcctc gcggtcgacg tgggggtccg
     5521 cgcgttcctg cccggctccc aggtggacct gcgtccggtc aagaacctgg ccgcgatggt
     5581 gggtcagacg atccgggcca aggtgatcaa gctcaaccga cggcgcggca acgtggtgct
     5641 ctcgcgccgc gcggtgctgg aagaggagcg cgaggagaag aagaagcaca ccctgtcggt
     5701 gctggccgag ggcatggtcc tcaccggcac cgtgaagaac ctgaccgact acggggcctt
     5761 catcgacctg ggcggcatcg acgggctgct gcacgtcacc gacatgtcgt ggggtcgggt
     5821 cgggcatccc tccgagatct tccagatcgg cgaccaggtc gaggtggtgg tgctgcactt
     5881 cgaccgcgag accggccggg tgtccctcgg ctacaagcag aagtcgtccg acccgtgggc
     5941 caacgtcgac gaccggtatc cggtcggggc caaggtcagc ggccgcatcg tgagcctcac
     6001 caactacggg gccttcgtgg agctggagcc cggagtggag ggcctcgtgc acgtctcgga
     6061 gatgtcgtgg acgcggcggg tgcgtcatcc ctcgaagctc gtcaacgtgg gggaccccgt
     6121 cgaggtcatg gtgctcgagg tcaacaaggc caccaagcgg atctccctcg gcatgaagca
     6181 ggtggagagc gatccgtggg ccaccatcac cgagcgctat cagcccggca atcgcgtgca
     6241 gggcaaggtg cgcaacctga ccgacttcgg cgccttcgtg gagctggagc cgggggtgga
     6301 cgggctcctg cacatctcgg acatgtcctg gacccgcaac atcgggcatc cctccgagct
     6361 gctcaagaag ggtcagccga tcgacaccca gatcctcaac gtggatcgcg acaacaagcg
     6421 catctcgctc gggctcaagc agatccagcc cgatccgtgg gacagcgtgg cgtcgcgcta
     6481 cccgatgggc tcccgggtca ccggcaaggt ggtgcgtctc accgacttcg gcgccttcgt
     6541 ggagctggag ccgggagtgg acgggctcct gcacatctcg cagatgtcga accggcccat
     6601 cgccaccccg gccgacatcc tgaacgtggg ggacgagctc accctcatgg tgatccgggt
     6661 ggatcccaac gagcggcgca tcgggctctc gctcaagga
//
LOCUS       DATMCV010000340         3364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_680852, whole genome shotgun sequence.
ACCESSION   DATMCV010000340 DATMCV010000000
VERSION     DATMCV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680852"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VNU03_07945"
     CDS             <1..1065
                     /locus_tag="VNU03_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74431.1"
                     /translation="QPMKTSGSTGLPLEFSWDRRNRDLYFASYLFSLEWAGTALWDTR
                     VTIAVPTYFSTNVEAASPLTRLARRLVLGEHTIGLGADTVTATALRSAIGGLGPRARY
                     FVRGYPAALTRVAGELLDAAASLPRDPRVVIAYSETLTASNAASIRRAFHCPVVNYYT
                     SWEIPQIAQTCPDRPDVLHVNGDRVALRVVLPDGSDALPGEAGRVVATDLTNEVMPFV
                     NYELGDRAVAGPPCACGRGWPTLLGVEGRDTERIRTPQGRHIHSGTLGHFLTFEAGAI
                     PQIWEYQALQVRLDHVVLLVVPTRAFSSEVAATLRERLQTFLGPGMSVAVEAVDRIPA
                     EASGKRLIIKSLIDDPTGRA"
     gene            complement(1028..1873)
                     /locus_tag="VNU03_07950"
     CDS             complement(1028..1873)
                     /locus_tag="VNU03_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP74432.1"
                     /translation="MAPHPDPWRAVDRQPDPASYVRMMESRGRTPTQARVRRRFLRFC
                     RLAPGWTVLEVGSGSGVVTRDVAARVGPRGHVIGIDKSRTLVDAARRRAGEAGLGRRV
                     TFRRGDAARLPFRSGRFDCALAVTVLLHVARPEAMLRELVRVTRTGGVVALQDQDFGT
                     QVLDHPDRALTRMILEGVTARMYPEPYSGRTLVRRLVELGLERVRLLADVYQVRTLDP
                     FTHGLLKRRAQQATEFGLIGAGRAARWLAAIEAADAHGHFVFTFNYYGASGLRPGRSD
                     RRSAT"
     gene            complement(1882..2721)
                     /locus_tag="VNU03_07955"
     CDS             complement(1882..2721)
                     /locus_tag="VNU03_07955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HWP74433.1"
                     /translation="MSGGRDGAAPAGYDLLVSDLDGTLLDDEMRLDPALVEAFHRAAA
                     RGLAISLATGRMPAAADPYRDELGITAPVIYYNGAMVRPTEGGPDLVSLTLPRGVLGR
                     AWTVVEQAPVHPIFYRDDRLYCAERTPAVRRYCEEEGLTVDVISDVREFLGLGSFIKS
                     LIIGHPRDLDIVREDLTPIVAEHGRLVRTRHDYLEIIPVAASKGAGLARLAEHVGVPL
                     ARVVAVGDQENDLEMLREAGLGVAMPHAPEPVRRAADRVAPAASEGGLLRLFAELFPR
                     HFA"
     gene            complement(2718..2963)
                     /locus_tag="VNU03_07960"
     CDS             complement(2718..2963)
                     /locus_tag="VNU03_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74434.1"
                     /translation="MRALLILACLLLVVMALSRLPSALKGRLPAAPPRRDELVKDPVC
                     QTYVLMSRAIREEAAGAPAYFCSRECAARFHQGERRA"
     gene            complement(2971..3189)
                     /locus_tag="VNU03_07965"
     CDS             complement(2971..3189)
                     /locus_tag="VNU03_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74435.1"
                     /translation="MDKLLRAIEVSMKATEASREAVQEILRRGAETGIFFSGGRGGVA
                     AEASPEGFDLTEQDRDFLRSLSIRPDPR"
BASE COUNT          470 a         1264 c         1197 g          433 t
ORIGIN      
        1 caaccgatga agacctcggg ctcaaccggg ctgccgctcg agttctcctg ggaccgccgc
       61 aaccgggatc tgtactttgc ctcgtacctg ttctcgctcg agtgggccgg gaccgccctc
      121 tgggacacgc gggtcacgat cgcggttccg acctacttct cgacgaacgt ggaggccgcc
      181 tcacccctca cccgcctcgc gcgccgtctg gtgctgggcg agcacaccat cggcctcggg
      241 gccgacaccg tcaccgcgac cgcgcttcgg tccgccatcg gcggcctggg accgcgggcc
      301 cggtacttcg tgcggggcta tcccgccgcg ctcacccggg tggcgggcga gctgctcgac
      361 gcggccgcgt ccctcccccg cgatccccgg gtggtcatcg cctattcgga gaccctgacc
      421 gcgagcaacg cggcctccat ccggcgcgcc ttccactgcc cggtggtgaa ctactacacg
      481 agctgggaga tcccccagat cgcccagacg tgtcccgacc ggccggacgt cctgcacgtc
      541 aacggcgatc gggtcgcgct ccgggtggtc cttcccgacg ggagcgacgc cctgcccggc
      601 gaggccggtc gggtggtggc gaccgatctg accaacgagg tgatgccctt cgtcaactac
      661 gagctcggcg accgtgcggt cgcgggcccg ccgtgcgcct gcgggcgagg ctggccgacc
      721 ctgctcggcg tcgagggccg agacaccgag cgcatccgga ccccacaggg gcgccacatc
      781 cacagcggga cgctcgggca cttcctcacc ttcgaggccg gggccatccc gcagatctgg
      841 gagtaccagg ccctccaggt ccgtctggac cacgtggtgc tgctcgtcgt cccgacgcgc
      901 gccttctcgt cggaggttgc cgccaccctg cgcgagcggc tccagacctt cctggggccc
      961 gggatgtcgg tcgccgtcga ggccgtcgac cggatcccgg ccgaggcctc gggaaagcgg
     1021 ctgatcatca agtcgctgat cgacgatccg accggccggg cctgaggccc gacgcaccgt
     1081 agtagttgaa cgtgaacacg aaatggccgt gcgcgtcggc ggcctcgatc gcggccagcc
     1141 agcgggcggc ccggcccgcc ccgatcaggc cgaactcggt ggcctgctgg gcccgccgct
     1201 tcagcagccc gtgggtgaac ggatcgaggg tccggacctg gtagacgtcg gccaggaggc
     1261 ggacccgctc gagccccagc tcgaccaggc ggcgcaccag ggtccgtccc gagtacggct
     1321 cgggatacat ccgggcggtc acgccttcga ggatcatgcg cgtgagggcg cggtccgggt
     1381 gatcgagcac ctgggtgccg aagtcctggt cctgcagcgc caccaccccg cccgtgcgcg
     1441 tcacccggac cagctcgcgg agcatcgcct cggggcgggc cacgtgcagg agtaccgtga
     1501 ccgccagcgc gcagtcgaag cggccggagc ggaacgggag ccgagccgcg tcgccccgcc
     1561 ggaacgtcac gcggcgtccg agccctgcct cgcccgcccg ccgccgcgcg gcgtccacca
     1621 gggtccggct cttgtcgatg ccgatcacgt gcccccgcgg ccccacccgc gcggccacgt
     1681 cccgcgtcac caccccgctg cccgagccga cctcgagcac cgtccaaccc ggcgcgaggc
     1741 ggcagaagcg caggaaccgg cgccgcaccc gcgcctgggt cggcgtccgg ccgcgcgact
     1801 ccatcatgcg gacgtagctc gcgggatcgg gctggcggtc gaccgcccgc cacggatcgg
     1861 ggtgcggcgc catgcgccgg ctcaggcgaa gtggcggggg aagagctcgg cgaagagccg
     1921 caggagcccg ccctcggagg cggccggcgc cacccggtcc gcggcccggc gcaccggctc
     1981 gggcgcgtgt ggcatggcga cgccgaggcc ggcctcgcgc agcatctcca gatcgttctc
     2041 ctggtcgccc accgccacca cccgcgcgag gggcacgccc acgtgctcgg cgagccgggc
     2101 cagacccgcg cccttggagg ccgcgacggg gatgatctcg agatagtcgt ggcgggtgcg
     2161 caccaggcgg ccgtgctcgg ccacgatcgg cgtcagatcc tctcgcacga tgtcgaggtc
     2221 gcggggatgg ccgatgatga ggctcttgat gaaggagccc aggcccagga actcgcgcac
     2281 gtccgagatc acgtcgacgg tgagcccttc ctcctcgcag tagcgccgga ccgccggggt
     2341 ccgctccgcg cagtagagcc gatcgtcgcg gtagaagatc gggtggaccg gggcctgctc
     2401 gaccaccgtc cacgcgcgac ccagcacccc gcggggcaag gtcagggaga ccaggtccgg
     2461 cccaccttcg gtcgggcgca ccatcgcgcc gttgtagtag atgaccggcg cggtgatgcc
     2521 cagctcgtcg cggtagggat cggccgccgc gggcatgcgt ccggtggcca gcgagatggc
     2581 gaggccgcgc gcggccgccc ggtggaaggc ctccaccagc gccgggtcta gccgcatctc
     2641 gtcgtcgagc agggtcccgt cgagatcaga gacgagcaga tcgtagccgg ccggcgccgc
     2701 gccgtcgcgc ccgccgctca cgcccggcgc tcgccctggt ggaagcgcgc cgcgcactcc
     2761 cggctgcaga agtaggccgg cgcgcccgcc gcctcctcgc ggatcgcgcg cgacatcagc
     2821 acgtaggtct ggcagaccgg gtccttgacc agctcgtcgc ggcggggcgg tgctgcgggc
     2881 agccggccct tcagggccga cggcaggcgc gagagggcca tcacgaccag gaggaggcag
     2941 gccaggatca gcagcgcgcg catggcgcga tcagcgggga tcgggacgga tggacagcga
     3001 ccggaggaaa tcgcgatcct gctcggtgag gtcgaagcct tccggagagg cctcggcggc
     3061 gaccccgccc cgaccgcccg agaagaagat gccggtctcg gccccgcgcc gcaggatctc
     3121 ctgcaccgct tcgcgggagg cctcggtggc cttcatcgac acctcgatgg cacggagcag
     3181 cttgtccatc agccgatccg cgggccgctc ttgcgccatg tgccttacga tagcacactg
     3241 acgcggcgag gcttcccgtg ccccccgacg ccggcgaccg cgagtggcgc ttcatccgca
     3301 gccacgagcg cgcgcgcatg ggcaagctca cgctgcgcga ggacgtctgg cggctgccca
     3361 ccgg
//
LOCUS       DATMCV010000341         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_883398, whole genome shotgun sequence.
ACCESSION   DATMCV010000341 DATMCV010000000
VERSION     DATMCV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883398"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VNU03_07970"
     CDS             complement(<1..192)
                     /locus_tag="VNU03_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP74436.1"
                     /translation="MADSELLYEVKDRIATITLNRPDKMNAFTGSMIERWAASLHEAQ
                     HDPNVNVVIVTGAGKAFCSG"
     gene            complement(210..1403)
                     /locus_tag="VNU03_07975"
     CDS             complement(210..1403)
                     /locus_tag="VNU03_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP74437.1"
                     /translation="MDFDFTQEQERFRQEVRAWLAENVPADLKGRGFASSRADVKEVE
                     RLRDWQRTLHKAGYVGMDWPAEYGGRGASIMESIILYEEMSRAGSPQPLNRSGLSMLG
                     PTLMKHGTPAQQAKHLDRILTAEDIWCQGFSEPNAGSDLANLQTRAVLDGDHYVLNGQ
                     KVWTSMAHVAAWGFFLVRTDPTLAKHKGITFLLLDMKSPGLTIRPLRQITGEAEFNEV
                     FLENVRVPATNVVGKVNEGWGVALTTLAYERDVLTMIRHISLRTALHKLLALAKRTMK
                     NGRPASEDPVVRQKIAALSIGERVLQLNGYRSLTKILKGGTPGPEGSTSKLFWSLLDQ
                     ELAELATEIIGPYSQIKAGSAHAPDEGEWEFYCLLARGSGIRAGTTEILRNILGERVL
                     GLPKD"
     gene            complement(1478..1867)
                     /locus_tag="VNU03_07980"
     CDS             complement(1478..1867)
                     /locus_tag="VNU03_07980"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HWP74438.1"
                     /translation="MLTLFLCAGVALAQSARPDPKTAAVPVMKQLEAFRRDDFDTAFT
                     FASGAIRAQFDRPSFETMVRRGYPEIARSSFAAVVKTELPSESVAYVTVKIRGANGQS
                     IEALYELTWEDDWKISGVVTRPDAGVI"
     gene            complement(1911..>2178)
                     /locus_tag="VNU03_07985"
     CDS             complement(1911..>2178)
                     /locus_tag="VNU03_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP74439.1"
                     /translation="GVTVNAICPGVLWTAFWEKLAQFIADTNPAFKGMTARQVFDKRV
                     GDVIPMKREQTPEDIGHAAVFLASEEARNITGQALNVDGGSVMH"
BASE COUNT          313 a          739 c          741 g          385 t
ORIGIN      
        1 gcccgagcag aaggccttgc ccgcgccggt cacgatcacc acgttcacgt tggggtcgtg
       61 ctgcgcctcg tgcagcgagg cggcccagcg ctcgatcatg gacccggtga aggcgttcat
      121 cttgtccggg cggttcaggg tgatggtggc gatgcggtcc ttcacctcgt agagcagctc
      181 gctgtcggcc atgggggtct ctccttggac tagtccttcg gcaggccgag cacgcgctcg
      241 cccaggatgt tgcgcaggat ctcagtggtg ccggcgcgga tgccgctgcc gcgcgcgagc
      301 aggcagtaga actcccactc gccctcgtcg ggcgcgtgcg cgctgcccgc cttgatctgc
      361 gagtaggggc cgatgatctc ggtggccagc tcggccagct cctggtccag caggctccag
      421 aaaagcttgg aggtcgagcc ctccgggccc ggcgtgccgc ccttcaggat cttggtgaga
      481 ctccgatagc cgttgagctg cagcacccgc tcgccgatgg agagcgcggc gatcttctgc
      541 cgcaccaccg ggtcctcgga ggcggggcgc ccgttcttca tggtgcgctt ggccagggcg
      601 agcagcttgt ggagcgcggt gcgcaggctg atgtggcgga tcattgtgag cacgtcgcgc
      661 tcgtaggcca gggtggtgag cgccacgccc cagccctcgt tcaccttgcc caccacgttg
      721 gtggccggca cgcgcacgtt ctcgaggaag acctcgttga actcggcctc gccggtaatc
      781 tggcgcagcg gccggatggt gaggcccggg ctcttcatgt cgagcaacag gaacgtgatg
      841 cccttgtgct tggccagggt cggatcggtg cgcaccagga agaagcccca cgcggccacg
      901 tgggccatgc tcgtccacac cttctggccg ttcagcacgt agtgatcgcc gtcgagcacc
      961 gcgcgggtct ggaggttggc caggtcgctc cccgcgttgg gctcggagaa gccctggcac
     1021 cagatgtcct ccgcggtcag gatgcggtcg aggtgcttcg cctgctgggc cggggtaccg
     1081 tgcttcatca gcgtgggccc cagcatgctg aggccgctgc ggttcagcgg ctgcgggctg
     1141 cccgcgcgcg acatctcctc gtagaggatg atcgactcca tgatcgaggc gccgcgcccg
     1201 ccgtactcgg ccggccagtc catgcccacg tagcccgcct tgtggagcgt gcgctgccag
     1261 tcgcgcaggc gctcgacctc cttgacgtcg gcgcgcgagg aggcgaagcc gcgccccttg
     1321 agatcggccg gcacgttctc ggccagccag gcgcggacct cctggcgaaa cctctcctgc
     1381 tcctgcgtga aatcgaagtc catcgcgctc ctagtcccct tctccctctc ccctgagggg
     1441 agagggcagg gtgaggggtg gtggtgctca gtgctcgtca aatgacccca gcatccggcc
     1501 tcgtcacaac cccgctgatc ttccaatcgt cctcccacgt gagctcgtac agcgcctcga
     1561 tgctctggcc gttggccccg cggatcttca ccgtgacgta ggcgacgctc tccgacggca
     1621 gctcggtctt gaccaccgcc gcgaacgacg accgcgcgat ctcggggtag ccgcgacgca
     1681 ccatcgtctc gaagctcggg cgatcgaact gcgcgcggat cgctcccgac gcgaaggtga
     1741 acgcggtgtc gaagtcgtcg cggcggaagg cctcgagctg cttcatcacc ggtaccgccg
     1801 cggtcttggg atcgggcctg gccgactgcg cgagagcgac acccgcgcag aggaatagtg
     1861 tgagcacggc ggcgaatccg aacgcgatgc gtttagccac ggcttcgacc ctagtgcatg
     1921 accgagccgc cgtcgacgtt cagggcctgg ccggtgatgt tgcgcgcttc ctcggaggcc
     1981 aggaagaccg cggcgtggcc gatgtcctcc ggcgtctgct cccgcttcat cgggatgaca
     2041 tcgcccaccc gcttgtcgaa aacctgccgc gcggtcatgc ccttgaaggc ggggttggtg
     2101 tcggcgatga actgggccag cttctcccag aacgcggtcc acagcacgcc ggggcagatc
     2161 gcgttcacgg tgaccccg
//
LOCUS       DATMCV010000342         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_351341, whole genome shotgun sequence.
ACCESSION   DATMCV010000342 DATMCV010000000
VERSION     DATMCV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351341"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VNU03_07990"
     CDS             <1..204
                     /locus_tag="VNU03_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HWP74440.1"
                     /translation="HMDNVLVSPHIASATARMAPETRRRLGREIATVLQGKWPRSAVN
                     PGVLPKTSLIRWQPYPMGRGPNR"
     gene            complement(361..1350)
                     /locus_tag="VNU03_07995"
     CDS             complement(361..1350)
                     /locus_tag="VNU03_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP74441.1"
                     /translation="MEYRVLGRTGLRVSALGFGCGDVGGLMVRGAPADRERGVARALE
                     LGINYLDTAPAYGSGESEKNLGQVLRALKPPVIVGTKWRLVAADLADVGGAVARSVET
                     SLGRLGLERVDLLHLHNLIGRVGEERPLGVARVLEAVVPAVRRLQEQGKVRFFGVTAS
                     GETGALHRALASGAIDTAQAVFNLLNPSGAYEVPAGYPAQDYDRLLTLAQEQRVGAIG
                     IRVLAGGALSGQVARHPTAIPSVAPIASGPDYATDARRARALEPLVAQGHAGSVVEAA
                     LRFAITGPALSTVLIGCSDLAQLEFAAAAVEKGPLPPAALASLHEIWGRLADH"
     gene            1450..>2323
                     /locus_tag="VNU03_08000"
     CDS             1450..>2323
                     /locus_tag="VNU03_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP74442.1"
                     /translation="MRHVRLGRTGLAVSGLCLGTMTFGLQCDEVASRAILDRAAAGGI
                     TFLDTADVYPVGGGFETVGRTEEIVGRWLEGRRHDFVVATKCCGAMSERRWDRGASRK
                     HILDAIDGSLRRLRTDYVDLYQLHHPDPATPIDESLRALDDVVRAGKARYVGCSNFPA
                     YQVARALGRSEVLGVARFDSVQPRYNLLFRQIERDLLPLCREEGMGVIPYNPLAGGLL
                     SGKHRRDSGPTPGTRFTLGNAAERYQDRYWHDREFETVETLRPLAAAAGISPTSLALA
                     WVLAEPGITAPIVGA"
BASE COUNT          300 a          899 c          827 g          297 t
ORIGIN      
        1 cacatggaca acgtgctggt gagcccgcac atcgcctccg cgacggcccg gatggcgccc
       61 gagacgcgcc ggcgcctggg ccgcgagatc gccaccgtgc tgcagggcaa gtggccccgc
      121 tccgcggtca acccgggcgt cctccccaag acctccctga tccgctggca gccctatccg
      181 atgggccgcg gccccaaccg ttagcgccgg gcggagctgg gggggcgggc tgccgggaac
      241 acgctgagtc gccgcgcttt gtgggcactc gagcgagcca cgagatggag gctcctcgcc
      301 ctggcgacgc gcagaagaca cggttcccgg cagcccgccc ccccagcccc gccctgacga
      361 tcagtggtcc gcgagtcgac cccagatctc gtgcaacgag gcgagggcgg cgggggggag
      421 cgggcccttc tcgaccgcgg cggcggcgaa ctcgagctgg gccaggtcgg agcagccgat
      481 caggacggtg gagagcgcgg gcccggtgat cgcgaagcgc agcgcggcct cgacgacgct
      541 gcccgcgtgg ccctgggcca ccagcggctc gagcgcgcgc gcccgccgcg cgtcggtcgc
      601 gtagtcgggc ccagaggcga tcggggccac gctggggatc gcggtcggat ggcgcgccac
      661 ctggccgctc agcgccccgc ccgcgagcac ccggatgccg atggcgccga cgcgctgctc
      721 ctgcgccagc gtgagcagcc ggtcgtagtc ctgcgcggga tagcccgcag gcacctcgta
      781 ggccccgctc gggttgagca ggttgaagac cgcctgcgcg gtgtcgatcg ccccgctcgc
      841 gagcgcgcga tggagcgcgc cggtctcgcc ggaggcggtg accccgaaga agcgcacctt
      901 gccctgctcc tgcagccggc gcaccgccgg gacgacggcc tccagcaccc gcgcgacccc
      961 gaggggccgc tcctcgccca cgcgcccgat caggttgtgg agatgtagca ggtccacgcg
     1021 ctcgagcccg agccggccga ggctggtctc gaccgagcgc gccaccgcgc cgcccacgtc
     1081 ggccaggtcg gccgcgacga gccgccactt ggtgcccacg atgaccggcg gcttgagcgc
     1141 gcgcagcacc tggcccaggt tcttctccga ctcgcccgag ccgtaggccg gtgcggtgtc
     1201 gaggtagttg atcccgagct cgagcgcgcg cgcgaccccg cgctctcgat cggcgggcgc
     1261 gccccgcacc atgagccccc ccacgtcgcc acagccgaac cccagcgccg acacccgcag
     1321 tcccgtccgc ccgagcaccc ggtattccat ggccgattct tataccactt ccggcgcgga
     1381 tcgcggcgtc cggctgggcc gcgccgtccg ttgcttgccc ccggcgcgcc ccttgggtac
     1441 tctcaccgca tgcgccacgt ccgcctcggc cgcaccgggc tcgccgtctc cggcctctgc
     1501 ctcggcacca tgaccttcgg cctgcagtgc gacgaggtcg cctcgcgcgc gatcctcgat
     1561 cgggcggccg cgggcgggat caccttcctc gacaccgcgg acgtttaccc ggtggggggc
     1621 ggcttcgaga cggtggggcg caccgaggag atcgtggggc gctggctcga gggccgacgg
     1681 cacgacttcg tggtggccac caagtgctgc ggggccatga gcgagcggcg gtgggaccgc
     1741 ggcgcgtcgc gcaagcacat cctggacgcc atcgacggct ccctgcgccg gctgcgcacc
     1801 gactacgtcg atctctacca gctccatcac ccggacccgg cgacgccgat cgacgagagc
     1861 ctgcgcgcgc tcgacgacgt ggtccgggcg ggcaaggccc gctacgtggg ctgctcgaac
     1921 ttccccgctt accaggtggc gcgggccctc gggcgcagcg aggtgctcgg ggtcgcgcgc
     1981 ttcgactcgg tgcagccgcg ctacaacctg ctcttccgcc agatcgagcg cgatctgctg
     2041 ccgctctgcc gggaggaggg catgggcgtc attccctaca acccgctggc gggcggcctg
     2101 ctgtccggca agcaccgtcg ggactcgggc cccaccccgg gcacccgctt cacgctgggc
     2161 aacgcggccg agcgctacca ggaccggtac tggcacgacc gcgagttcga gaccgtcgag
     2221 accctgcgcc cgctcgctgc cgccgccggc atctcgccga cgagcctggc cctggcctgg
     2281 gtgctcgcgg agcccgggat caccgcgccg atcgtgggcg cga
//
LOCUS       DATMCV010000343         6334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_753513, whole genome shotgun sequence.
ACCESSION   DATMCV010000343 DATMCV010000000
VERSION     DATMCV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753513"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VNU03_08005"
     CDS             <1..377
                     /locus_tag="VNU03_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWP74443.1"
                     /translation="FAAGVMVPEAVAASRALAATGICASVFAVTSPDLLYRGLRATRP
                     YVLDLVSADEEGVPVVSVLDGHSHALAFLGSALGVPQLPLGVDDFGQSGSRSDLYRHY
                     GVDAAAIARAAHSLLAGGPSLS"
     gene            436..1797
                     /locus_tag="VNU03_08010"
     CDS             436..1797
                     /locus_tag="VNU03_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP74444.1"
                     /translation="MDSRLKNLAEQLDAGLIARREFLRKAAIVTGGTAAGLHALRGMA
                     SAQAKTKLRLWLFKSFVTAGNDVVAKQVETWAKEKNVDVEMDWATFGDREQKFVAAIE
                     AGNPPDMAEMNLYGPMRYKAALRDVTKVAGDVAASKGGLLPFAERLMKVDGKFLGVAR
                     YSMTTVFFIRKDVMEAKGLKVPKVYDPDVVEFAKKSQDPAKDLWGFGQTLNRSDDGDG
                     FMSNILWDYGGGVFDKDGKPAIGTTFLKQNTAALQFAVDTIKKHGIQPPGVMGWTDVS
                     NNEAYMAGKLVTTNNGASLYYAMVSKKHELAPKTTLVLTPGGPAGSFVGSSCYNWAIF
                     HKSAKAELCEDLIRYVEDEKRFAEYIQVSVGQAGPVYKGRVDNPYWKSDPNFDAILQN
                     ILRSVNIGYPGPMTPAAAEVQGQKILTDMAGRVVVGGLSPEAALKEAHARVEEIHKIR
                     GKA"
     gene            1858..2808
                     /locus_tag="VNU03_08015"
     CDS             1858..2808
                     /locus_tag="VNU03_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWP74445.1"
                     /translation="MRGGFHPDVAPRPRPRAALEVTLRRLLGPDYRMGFLFVAPIVVL
                     VLALVAYPFCYAVYLSMTRKYVGMPPVWVGLENYIRLTYDGFFQRAVVNSFTFTFASV
                     AIKTVLGMGMALVLTSKIRFRSFWTGVLLIPWVAPTVVSALNFLWIYDYSLGVLNYLL
                     VHVFGVLSHGVGWLSEPGTAMSSVIGVNVWRGFPFFGISFLAGMKAIPGEQYEAAAVD
                     GATVLQRFRHVTLPGIRNIVMIVLLLSTIWTFNDFAIIYILTKGGPGGATMVLPVLTY
                     EIAFGAQRLGEAIAVALYMLPPLAFVIIVLSNYMRKGRAR"
     gene            2855..3712
                     /locus_tag="VNU03_08020"
     CDS             2855..3712
                     /locus_tag="VNU03_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWP74446.1"
                     /translation="MIVRKGRSTVQIERTQSAVSYAILITFVLFVVFPFYWMIVTSFK
                     GETQMRSVVSMFWPSPFVAENYEHLFKKTEFVSWFGNSVIVAVSSTFLATAVGTLGAY
                     ALARLKFLGRAFMASTVLITYLVPPSILFIPLYAQIRNLGMADSLTGLIAAYPSFTVP
                     FVTWLLMGYFESIPVELEESAMIDGATRFGAFRRVILPLAAPGLLASALYAFTQAWNE
                     FLYALVFITNVKLRTLPVGLSTFITGDVYGWGYLMAGAVLTTLPVIAAYIYLQKYMVE
                     GLTAGSVKG"
     gene            complement(3687..4409)
                     /locus_tag="VNU03_08025"
     CDS             complement(3687..4409)
                     /locus_tag="VNU03_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWP74447.1"
                     /translation="MRTPPPPDTGPTGPPARTYRLLLGHAMALVRRGRIPSVPELAVK
                     ARVSRATAYRYFPSRSKLVSAVVAESLAPVRRFEPAAADGLVRVRELFDKTFPLFKRF
                     EPHMRAALQLSLEHESLERTGLLEEEPYRRGHRRYMLHRAAAPLAATLGPEAYERLLK
                     ALSLIYGIESYVVLRDIWGASYREVEVVARWMVEALIESALRRAPHPAAPHPPAPHLA
                     APHPALSPKGRGRKPSPSRSRR"
     gene            4570..5856
                     /locus_tag="VNU03_08030"
     CDS             4570..5856
                     /locus_tag="VNU03_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP74448.1"
                     /translation="MTSVLFTNVRIFDGTGALAYPGEVLVQGNRISRIARGARALPTA
                     GVATVDGGGATLMPGLVEAHTHFSWNDQPGLSDIQRMPTEEHILWCAHIAKRYLDMGF
                     TSCIGAATAKPRLDVVIRNAIESGQIPGPRYLAASQEITVPGGLGDETLPHLPYPEFS
                     FGAIVNGPEEMRKTVRMFLKYGVDTVKLNLSGEYIAGIPAEFTPMTEEEIAVAVKEAH
                     VRGKRVSAHARSAESVKQCVRHGIEIIYHASFADEEALDMLEAKKEHHFVAPGIAWLI
                     NTSFHAAPWGISPDAAKQMGYHRELEVACETLKKMHRRGIRILPGGDYGFAWIKHGTN
                     AKDLEYFVKYLGFTPMEALLAATRLGGQIMMRGDELGRIQEGHLADLLLVDGDPVADI
                     TVLQDGARLLGVMKDGAFHKDPQKGPARGRFSRSAA"
     gene            5876..>6334
                     /locus_tag="VNU03_08035"
     CDS             5876..>6334
                     /locus_tag="VNU03_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP74449.1"
                     /translation="MTTQFVERIAVETDGSGEAVLMIHGLGGTSNVWTPVLPAMGHHR
                     TIRPDLPGSGRSHRVEGPLSIERFVQAMQRVCAVLGLERVHVAAHSLGTIVAFHLAVA
                     APRLVRSLALFGPVLCPPDSARPAIRARGEKARAEGEAGMQAIADALVQGA"
BASE COUNT          975 a         2194 c         2170 g          995 t
ORIGIN      
        1 tcttcgcggc cggcgtgatg gtgcccgagg cggtggcggc gagccgcgcg ctggccgcga
       61 ccgggatctg cgcgagcgtc ttcgcggtca cgagcccgga cctgctctac cgcggtctgc
      121 gcgctacgcg gccctacgta ctcgacctgg tgtcggcgga cgaggagggc gtgccggtgg
      181 tgtcggtgct ggacggccac tcccacgcgc tggccttcct gggcagcgcg ctcggcgtcc
      241 cgcagctgcc gctcggggtg gatgacttcg gccagtcggg ctcgcgtagc gatctctacc
      301 gccactacgg cgtcgacgcc gcggccatcg cgcgcgccgc gcacagtctc cttgccgggg
      361 gcccctccct gagttagcat ccgatcatcc ccgccgcact ccaccgcggc gccgcgctcc
      421 aaaggaggag cctccatgga cagccgcctc aagaatctgg ccgagcagct cgacgcaggc
      481 ctcatcgcgc gccgtgagtt tttgcgaaag gcggcgatcg tcaccggggg caccgcggcc
      541 ggcctgcacg cgctccgtgg catggcctcc gcccaggcca agaccaagct gcgcctctgg
      601 ctgttcaaga gcttcgtcac cgcgggcaac gacgtggtgg ccaagcaggt cgagacctgg
      661 gccaaggaga agaacgtcga cgtggagatg gactgggcca ccttcggcga ccgcgagcag
      721 aagttcgtcg ccgccatcga ggcgggcaac ccgcccgaca tggccgagat gaacctctac
      781 gggccgatgc gctacaaggc cgcgctccgc gacgtcacca aggtcgcggg cgacgtcgcc
      841 gcgagcaagg gcgggctgct gcccttcgcg gagcgactga tgaaggtcga cggcaagttc
      901 ctcggcgtcg cgcgctactc gatgaccacc gtgttcttca tccgcaagga cgtgatggag
      961 gccaaggggc tcaaggtccc gaaggtctac gatccggacg tggtggagtt cgccaagaag
     1021 tcccaggacc ccgccaagga tctgtggggc ttcggccaga ccctgaaccg ctccgacgac
     1081 ggcgacgggt tcatgtccaa catcctctgg gactacggcg ggggcgtgtt cgacaaggac
     1141 ggcaagccgg ccatcggcac gaccttcctc aagcagaaca ccgccgcgct gcagttcgcg
     1201 gtcgacacca tcaagaagca cggcatccag ccgcccggcg tgatgggctg gaccgacgtg
     1261 tccaacaacg aggcctacat ggcgggcaag ctggtcacca ccaacaacgg ggccagcctc
     1321 tactacgcga tggtgtcgaa gaagcatgag ctggcgccca agaccaccct ggtgctgacc
     1381 ccgggcggcc ccgcgggcag cttcgtgggc tcgtcgtgct acaactgggc catcttccac
     1441 aagtcggcga aggccgagct ctgcgaggac ctcatccgct acgtggagga cgagaagcgc
     1501 ttcgccgagt acatccaggt gtcggtgggg caggcggggc cggtctacaa gggccgggtc
     1561 gacaatccct actggaagtc ggatccgaac ttcgacgcga tcctgcagaa catcctccgc
     1621 agcgtcaaca tcggctatcc gggccccatg acgcccgcgg ccgccgaggt gcaggggcag
     1681 aagatcctga ccgacatggc cggtcgcgtg gtggtgggcg ggctctcgcc cgaggccgcg
     1741 ctgaaggaag cccacgcgcg ggtcgaggag atccacaaga tccgcggcaa ggcctagctt
     1801 cagatggcca ccgtccatcc ctcgtccccc cctctccccg gaggcgagag ggtcggggtg
     1861 aggggcggct tccaccccga cgtcgcgccc cggcctcggc cccgcgccgc gctcgaggtc
     1921 acgctgcggc gtctcctcgg ccccgactac cgcatgggct tcctcttcgt ggcgcccatc
     1981 gtggtgctgg tgctggccct ggtcgcgtac ccgttctgct acgcggtgta cctcagcatg
     2041 acccgcaagt acgtgggcat gccgccggtg tgggtcggcc tcgagaacta catccgcctc
     2101 acctacgacg gcttcttcca gcgcgcggtc gtcaacagct tcacgttcac gttcgcctcc
     2161 gtcgcgatca agacggtgct gggcatgggc atggcgctgg tgctcacctc gaagatccgc
     2221 ttccgctcgt tctggaccgg ggtgctcctg attccgtggg tggcccccac cgtggtttcc
     2281 gcgctcaact tcctgtggat ctacgactac agcctgggcg tgctgaacta cctgctggtc
     2341 cacgtgttcg gcgtgctctc gcacggggtg ggctggctca gcgagcccgg caccgcgatg
     2401 tcctccgtca tcggggtcaa cgtgtggcgc ggcttcccgt tcttcgggat cagcttcctg
     2461 gccggcatga aggcgattcc cggggagcag tacgaggcgg ccgcggtgga cggggccacc
     2521 gtgctccagc gcttccgcca cgtcacgctg cccggcatcc gcaacatcgt gatgatcgtg
     2581 ctgctgctct cgacgatctg gaccttcaac gacttcgcga tcatctacat cctgaccaag
     2641 ggcgggccgg gcggcgccac catggtcctg ccggtgctga cctacgagat cgcattcggg
     2701 gcccagcgcc tgggcgaggc catcgcggtc gcgctctaca tgctgccccc gctcgccttc
     2761 gtgatcatcg tcctgtcgaa ctacatgcgc aaaggtaggg cgaggtgagt ccgttcgacg
     2821 gacgagacga gcgaatacaa gagtggggtc agggatgatc gtccgcaagg gccgcagcac
     2881 cgtccagatc gagcggaccc agtccgcggt ctcctacgcg atcctgatca cgttcgtcct
     2941 gttcgtggtg ttcccgttct actggatgat cgtcacgtcc ttcaagggcg agacccagat
     3001 gcgcagcgtg gtctcgatgt tctggccgag cccgttcgtg gccgagaact acgagcacct
     3061 gttcaagaag accgagttcg tctcctggtt cggcaacagc gtcatcgtcg cggtgtcgag
     3121 caccttcctg gccaccgcgg tgggcacgct cggcgcctac gcgctggccc ggctcaagtt
     3181 cctggggcgg gcgttcatgg ccagcacggt gctgatcacg tacctggtgc cgccgtcgat
     3241 cctgttcatc ccgctctacg cccagatccg caatctgggc atggccgaca gcctcaccgg
     3301 gctgatcgcg gcgtacccgt ccttcacggt gccgttcgtg acctggctgc tgatgggcta
     3361 cttcgagtcg atcccggtgg agctggaaga gtccgccatg atcgacgggg ccacgcgctt
     3421 cggcgcgttc cgccgggtga tcctgccgct ggccgccccc ggcctgctcg cgagcgcgct
     3481 ctacgccttc acccaggcct ggaacgagtt cctctacgcc ctggtcttca tcaccaacgt
     3541 gaagctgcgc acgctgccgg tgggcctctc caccttcatc accggcgacg tctacggctg
     3601 gggctacctc atggcgggcg cggtcctgac caccctgccg gtcatcgcgg cctacatcta
     3661 cctccagaag tacatggtgg aaggcctcac cgccgggagc gtgaagggct aggctttctc
     3721 cctctcccct ttggggagag ggcagggtga ggggctgcaa ggtgaggggc tggtgggtga
     3781 ggggctgccg ggtgaggggc ccgccgcagc gccgactcga tcaacgcctc caccatccac
     3841 cgcgcgacga cctccacctc gcggtagctc gccccccaga tgtcccgcag caccacgtac
     3901 gactcgatcc cgtagatcag cgacagcgcc ttgagcagcc gctcgtaggc ctccggcccc
     3961 agcgtggcgg ccagcggggc ggcggcccgg tgcagcatgt agcggcggtg cccgcgccgg
     4021 tagggctcct cctccagcag ccccgtccgc tcgagcgact cgtgctccag cgagagctgc
     4081 agggccgccc gcatgtgggg ctcgaagcgc ttgaacaggg ggaaggtctt gtcgaacagc
     4141 tcgcgcaccc gcaccaggcc gtcggccgct gcgggctcga agcggcgcac cggggccagg
     4201 ctctccgcca ccaccgcgct cacgagcttg ctgcggctcg ggaagtagcg gtacgcggtg
     4261 gcgcgcgaga cgcgggcctt caccgcgagc tccgggaccg acgggatgcg cccgcgccgc
     4321 accagcgcca tcgcgtggcc gagcagcagc cggtaggtgc gcgccggggg accggtcggg
     4381 ccggtgtcgg ggggcggcgg ggtcctcacg gctcgctact gtgaccgcgt aattgccggt
     4441 tgacaagagg ggaaggcact tcctagtatg acccactcgc gtgagacagg tgtctcatgg
     4501 aatccggagc tgcccaccgc cgcgcagcac ccgacccgag gcggggccgc tcccaggcag
     4561 gagggcggca tgaccagcgt tctgttcacg aacgtccgga tcttcgacgg gaccggcgct
     4621 ctggcgtatc cgggagaggt cctcgtccag ggcaaccgca tctcccgcat cgcgcgcggg
     4681 gcccgggcgc tgcccaccgc gggggtcgcc accgtggacg ggggcggcgc caccctgatg
     4741 cccggcctcg tggaggcgca cacccacttc tcctggaacg accagcccgg cctcagcgat
     4801 atccagcgca tgcccaccga ggagcacatc ctctggtgcg cccacatcgc caagcggtat
     4861 ctcgacatgg gcttcacctc gtgcatcggc gccgccaccg ccaagccgcg cctcgacgtc
     4921 gtgatccgga acgccatcga atcgggccag atcccgggcc cgcgctacct ggccgccagc
     4981 caggagatca cggtgccggg cggcctgggc gacgagacgc tgccgcacct gccgtatccg
     5041 gagttcagct tcggggccat cgtcaacggg cccgaggaga tgcggaagac ggtgcgcatg
     5101 ttcctcaagt acggggtgga caccgtcaag ctgaacctct cgggggagta catcgcgggg
     5161 atcccggccg agttcacgcc gatgaccgag gaggagatcg cggtcgcggt gaaggaggcc
     5221 cacgtgcgcg gcaagcgggt ttcggcccac gcgcgatcgg cggaatcggt gaagcagtgc
     5281 gtgcgccacg gcatcgagat catctaccac gcgagcttcg cggacgagga agcgctcgac
     5341 atgctggagg cgaagaagga gcatcacttc gtcgcccccg gcatcgcgtg gctcatcaac
     5401 acctcgttcc acgcggcccc gtggggcatc tcgcccgacg ccgccaagca gatgggctac
     5461 caccgcgagc tcgaggtcgc ctgcgagacc ctcaagaaga tgcaccggcg cgggatccgc
     5521 atcctgcccg ggggcgacta cggcttcgcg tggatcaagc acggcaccaa cgcgaaggat
     5581 ctcgagtact tcgtcaagta cctgggcttc accccgatgg aggcgctcct ggccgcgacc
     5641 aggctcgggg gtcagatcat gatgcggggc gacgagctcg gccggatcca ggaggggcat
     5701 ctcgccgacc tgctgctggt cgacggcgac ccggtcgccg acatcacggt gctgcaggac
     5761 ggggcgcggc tgctcggcgt catgaaagac ggcgcgttcc acaaggatcc gcagaagggg
     5821 ccggcgcgcg ggcgcttcag ccggtccgcc gcgtgaggat gggcggaagc tccgcgtgac
     5881 cacgcagttc gtcgagcgca tcgcggtcga gaccgacggg agcggcgagg cggtgctgat
     5941 gatccacggc ctcggcggga cttccaacgt gtggacgccg gtcctgcccg cgatgggtca
     6001 ccaccggacg atccgtccgg acctgcccgg gtcgggccgc tcgcatcggg tcgagggccc
     6061 gctctcgatc gagcgcttcg tccaggcgat gcagcgcgtg tgcgcggtgc tcggcctgga
     6121 gcgggtgcac gtggccgccc actcgctcgg caccatcgtg gcctttcact tggcggtggc
     6181 ggccccgcgg ctggtacgga gccttgcgct cttcggcccg gtgctgtgcc cgccggacag
     6241 cgcgcggccg gccatccgcg cgcgagggga gaaggcgcgc gcggaagggg aggcgggcat
     6301 gcaggccatc gccgacgcgc tggtgcaggg cgcg
//
LOCUS       DATMCV010000344         4047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_86064, whole genome shotgun sequence.
ACCESSION   DATMCV010000344 DATMCV010000000
VERSION     DATMCV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86064"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VNU03_08040"
     CDS             <1..219
                     /locus_tag="VNU03_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HWP74450.1"
                     /translation="LKARVIARHVSLIREHFREKYALIQMKKHLAWYTEGLGHATECR
                     ARIFQTRSPEEVWDVFQSYWTSTRGLVA"
     gene            complement(381..1058)
                     /locus_tag="VNU03_08045"
     CDS             complement(381..1058)
                     /locus_tag="VNU03_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74451.1"
                     /translation="MPAAPYLIAMCDVLGFTRLVATTPLDEIHERYRALLAQLEPHPL
                     FRIKAGTYAREIVLNRVVFSDTILMWAPAGEAMELLPGVLGQIMGRAMGSMPLRAGLA
                     FGECVIDPANELYVGQPIIDAYHTEQSQEWVGGAFHRSCWRRPGLRDVLCRGYEGSAV
                     KYPVPVKAGAEPLEYALNWPALAGPDFTVADLVASEGGAAPGPKVKWQNARRFYDEVA
                     ANRGSSR"
     gene            complement(1073..1261)
                     /locus_tag="VNU03_08050"
     CDS             complement(1073..1261)
                     /locus_tag="VNU03_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74452.1"
                     /translation="MEIDPHDRRLHTYEYRTIKGAKGEKVFESHVETKATAAAHRVFW
                     HHGPLPDYIIVVAITAYP"
     gene            complement(1381..1698)
                     /locus_tag="VNU03_08055"
     CDS             complement(1381..1698)
                     /locus_tag="VNU03_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74453.1"
                     /translation="MAIKLDELEGIGETIADSKARVTLARALRQLRARFGDRKIRFNV
                     FIEESTGAIVLAPATTVPLREAWLHENPKALKAVRTGIAQAGRGEAIKGRSFAKHADD
                     DAG"
     gene            complement(1795..2895)
                     /locus_tag="VNU03_08060"
     CDS             complement(1795..2895)
                     /locus_tag="VNU03_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HWP74454.1"
                     /translation="MNRAEIPTPALLLDLDRFERNLQKMAAHVKAAGKKLRPHAKTHK
                     CPEIARRQIAAGAVGVCVAKVGEAEVMAAAGVRNLLITTEVVGPEKIGRLLGVLRRQP
                     ETLVVVDHPDNVRELGAAVAKAGLVLNVLVDVDVGGRRTGAQPGEPARDLGREVMAQP
                     ALHLRGLQGYAGHCAHVMGFDDRRRTSRRWMGRLMKTRELFEKHGMAVDIVTGGSSGT
                     FNIDSELEGMTELQSGSYCVMDLDYRRIGSPRGAAYDEFEMALTVLTTVVSVPSQEMA
                     MVDGGFKAFSTDRPFVPEAVEWPGVEYSWAGDEHGRLMTAEPERLPKLGQLIEFFPPH
                     CDPTINLYDRIYAMRGSTVEAVWEVSGRGRSQ"
     gene            complement(2950..3459)
                     /locus_tag="VNU03_08065"
     CDS             complement(2950..3459)
                     /locus_tag="VNU03_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74455.1"
                     /translation="MKHRTQVALLLLFLLTTAVALPVIPTAEAAGVDWAAEIQAMDAA
                     LARGDAANAQAAWRDAYAAAHVGREWPGMVAVGDAALRLGRATDTGAVAESRARRVYL
                     TALFRARRHGSLDGVLAAGEAFGRLGDRAVVQQALAMATEMARRSGDDLAQRRVQAFR
                     SQWLARPLT"
     gene            3641..>4047
                     /locus_tag="VNU03_08070"
     CDS             3641..>4047
                     /locus_tag="VNU03_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HWP74456.1"
                     /translation="MNLLVAVIPSALICLWPLVALAQSFNPDRPDLTTSAELVPVGAL
                     QIETGLEYERARVGGGPTQQQISTQAVLRAGVATVLEVSLEGEPFVWQRADHDSAGSG
                     DYTLGLKYRLYAPPEGSSGPLVAVKPFVKLPAAR"
BASE COUNT          567 a         1481 c         1398 g          601 t
ORIGIN      
        1 ctcaaggcgc gggtcatcgc ccgccacgtg agcctcattc gcgagcactt ccgggagaag
       61 tacgcgctga tccagatgaa gaagcacctc gcgtggtaca cggaggggct cggccacgcc
      121 accgagtgcc gcgcccgcat cttccagacc cgcagccccg aggaagtctg ggacgtgttc
      181 cagtcctact ggacgtccac gcgcggcctc gtcgcctaga ttcgtcggcg cgggcgcgtt
      241 caacgccgcc ttcacggtca acggcgagct ggagccggtc ccggagccgg ccaccctggc
      301 cctgctcggc actactatgg tggatctcgg cgccgcgggt tggaggcggc gccgggagcg
      361 gtagcccgct ccaccggctc ctaccgggaa gagccccggt tggccgcgac ctcgtcgtag
      421 aaccgccggg cgttctgcca ctttaccttc gggcccggcg ccgcgcctcc ctccgaggcg
      481 acgagatccg cgaccgtgaa gtcgggaccg gccagcgcgg gccagttcag ggcgtactcg
      541 agcggctcgg ccccggcctt cacgggaacc gggtacttca ccgcactccc ctcgtagccc
      601 cggcacagca cgtcgcgaag gccgggccgg cgccagcagg atcggtgaaa cgcgccgccg
      661 acccattcct gcgactgctc ggtgtgatag gcgtcgatga tcggctgccc gacgtagagc
      721 tcgttggccg gatcgatgac gcactcgccg aaggccaggc ccgcgcggag cggcatcgag
      781 cccatcgccc tccccatgat ctgccccagc acgcccggaa ggagctccat cgcctcgccg
      841 gcgggcgccc acatgaggat cgtgtcgctg aacacgacgc gattgagaac gatctcgcgc
      901 gcgtaggtcc cggccttgat gcggaacagg ggatgcggct cgagctgggc gagcagcgcc
      961 cggtaccgct cgtggatctc gtcgagcggg gtcgtggcca ccaggcgggt gaaccccagc
     1021 acgtcgcaca tggcgatcag atacggggcg gcgggcatcc ggggcccgat cctcagggat
     1081 acgcggtgat ggccacgacg atgatgtagt ccggcagcgg cccgtggtgc cagaagactc
     1141 gatgggccgc ggcggtggcc ttggtctcca cgtgggactc gaagaccttc tcgcccttcg
     1201 cgcccttgat cgtgcggtac tcgtaggtgt gcagcctacg gtcgtgcgga tcgatctcca
     1261 tgagagcgag agtcttgcgg accgcggtca gccgcttcgc ccgcttcgga tcgtgctcga
     1321 gggccgtcag ctcctggtcg gcgaccggtg tgcagaggag ggtgaagcgc gggatcggag
     1381 ctacccggcg tcgtcgtccg cgtgcttggc gaacgatctg cccttgatcg cctcaccgcg
     1441 gccggcctga gcgatgccgg tccggaccgc cttcagcgcc ttggggttct cgtggagcca
     1501 cgcctcccgc agcggtaccg tcgtcgccgg agcgaggacg atcgcgcccg tcgactcctc
     1561 gatgaagacg ttgaaccgga tcttgcggtc accgaagcgg gcccggagct gccggagggc
     1621 gcgcgccagc gtcacgcgcg ccttgctgtc cgcgatggtc tccccgatgc cctcgagctc
     1681 atccagcttg atggccatgg cgtggctcct tagtgggatt atcccacctc cccactccct
     1741 gtcaaccccg cccgacctcc cgggcccagc tggtatatgc ccgtctctac aggcttattg
     1801 ggaccgtccc cgcccggaga cctcccagac cgcctccacc gtgctcccgc gcatcgcgta
     1861 gatgcggtcg tagaggttga tggtggggtc gcagtgcggc gggaagaact cgatgagctg
     1921 cccgagcttg ggcagccgct ccggctcggc ggtcatgagc cgcccgtgct cgtcgccggc
     1981 ccaggagtac tccacgcccg gccactccac cgcctccggc acgaagggcc ggtccgtcga
     2041 gaaggccttg aagccgccgt ccaccatcgc catctcctgg ctcggcacac tcaccaccgt
     2101 ggtcagcacg gtgagcgcca tctcgaactc gtcgtaggcc gccccgcgcg ggctgccgat
     2161 gcgccggtag tcgaggtcca tcacgcagta ggagccggac tgcagctcgg tcatcccctc
     2221 gagctcgctg tcgatgttga aggtgccgct cgagccgccg gtcacgatgt ccaccgccat
     2281 gccgtgcttc tcgaacagct cgcgcgtctt catgagccgg cccatccagc gccgcgaggt
     2341 gcgccgccgg tcgtcgaagc ccatcacgtg cgcgcagtgg cccgcgtagc cctgcagccc
     2401 gcgcaggtgg agcgcgggct gggccatcac ctcgcgaccc aggtcgcgcg cgggctcgcc
     2461 gggctgggcg ccggtccgcc ggccgcccac gtcgacgtcc accagcacgt tgagcacgag
     2521 gccggccttg gccaccgccg cccccagctc ccgcacgttg tccgggtggt ccaccaccac
     2581 gagcgtctcg ggctgtcgcc gcaggacgcc cagcagccgc ccgatcttct ccgggcccac
     2641 cacctcggtc gtgatcagga gattgcgcac gcccgcggcc gccatcacct cggcctcgcc
     2701 caccttggcc acgcagacgc cgaccgcgcc ggccgcgatc tggcggcggg cgatctcggg
     2761 gcacttgtgg gtcttggcgt gggggcggag cttcttgccg gccgccttca cgtgggcggc
     2821 catcttctgg agattacgct cgaagcgatc gaggtccagc agcagcgcgg gggtggggat
     2881 ctcggcgcgg ttcatggcgg ggggagcata ccacgcgccg gaatccgctc ccgagccggc
     2941 cgccggcgat caggtgagcg gacgcgccag ccactggctg cggaaggcct gcacgcgtcg
     3001 ttgcgccaga tcgtccccgg agcgcctggc catctcggtg gccatcgcga gggcctgctg
     3061 caccaccgcg cggtcgccga gccggccgaa cgcctcgccg gccgccagca ccccgtccag
     3121 cgagccgtgc cggcgcgcgc ggaacagggc ggtcaggtag acgcgccggg cgcggctctc
     3181 cgcgaccgca cccgtgtcgg tggcgcgacc gagccggagg gccgcgtcgc cgaccgccac
     3241 catgccgggc cattcgcgcc cgacgtgggc ggcggcgtag gcatcccgcc acgcggcctg
     3301 ggcgttggct gcgtcgcccc gcgcgagggc tgcgtccatc gcctggatct cggcggccca
     3361 gtcgaccccg gccgcctcgg cggtcgggat gaccggcagc gccaccgcag tcgtcaggag
     3421 gaacaggagg agcagcgcga cctgcgtccg gtgtttcatg ggagcctcct cggctggatc
     3481 ggcatccgcc gggaaggcaa gcaccggcca tgccagtggg caagtatctg gaaacccgtc
     3541 ggaagctccc gagaaccgct cgtcgacgga cgggcatgac gcccggcgct ggcagaatgc
     3601 ctgacgccgg gttggggccc accacatgta tgatggccgc ttgaacctcc tggttgccgt
     3661 catcccgagc gcgctgatct gcctgtggcc cctggtggcc ctcgcccaga gcttcaaccc
     3721 ggaccggcct gatctcacca ccagcgccga gctggtcccg gtgggcgcgc tgcagatcga
     3781 gaccggcctc gagtacgagc gcgcgcgggt cggcggcggg cccacccagc agcagatcag
     3841 cacccaggcg gtgctgcgcg cgggcgtggc gaccgtgctc gaggtctcgc tggagggtga
     3901 gccgttcgtg tggcagcgcg ccgaccacga ctccgccggg agcggcgact acacgctcgg
     3961 cctcaagtac cgcctttacg ccccgcccga agggagtagc ggcccgctcg tggccgtgaa
     4021 gccgttcgtg aagctgccgg ccgcccg
//
LOCUS       DATMCV010000345         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_883769, whole genome shotgun sequence.
ACCESSION   DATMCV010000345 DATMCV010000000
VERSION     DATMCV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883769"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VNU03_08075"
     CDS             complement(<1..213)
                     /locus_tag="VNU03_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWP74457.1"
                     /translation="MSSARRVASLDEVPAGQPKLVELDGTRIVLARVGSAVYACGDVC
                     AHRGGPLSEGKLTGTRLACPWHGWMYD"
     gene            complement(210..893)
                     /locus_tag="VNU03_08080"
     CDS             complement(210..893)
                     /locus_tag="VNU03_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWP74458.1"
                     /translation="MTNASPFRTQLEHAVNARHSRLNPFTEKWVNGELTRAQLGQWVC
                     QHYQYVSQFARWCAIVYGGCPDPDARDFLLENIIEEESGTKHVDLLIRFGEACGVSRA
                     EVEGTRQLPSTRALTAWCYEMSRKPFHVAAAGLLVGLESQVPGIYKRNLPPLRTHYGF
                     NDHEVEFFAIHIEADEVHGERGYQIVERHSPTPEKQAEAAEAVREATEMRWQYMTGLH
                     RAFVLKEDA"
     gene            complement(919..1422)
                     /gene="purE"
                     /locus_tag="VNU03_08085"
     CDS             complement(919..1422)
                     /gene="purE"
                     /locus_tag="VNU03_08085"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWP74459.1"
                     /translation="MSAGSAAPLVGILMGSKSDWETMAHAAKALDALGVAYEVNVMSA
                     HRTPALVDEYVTSAEGRGLEVIIAGAGGAAHLAGVCAARTLLPVLGVPMQSKALNGMD
                     SLLSTVQMPAGIPVGTLAIGQAGATNAGLLAGAILAGKHPPIRAALRKYRDSQTQALL
                     AQTDPRA"
     gene            1734..>2079
                     /locus_tag="VNU03_08090"
     CDS             1734..>2079
                     /locus_tag="VNU03_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWP74460.1"
                     /translation="MTTETSEQLLREMVRIRRIEETLAELYPSQEMRTPTHFSIGQEA
                     VAVGVCSVLRRTDAVYSGHRCHAHYLAKGGDLGGMVAELYGRETGCARGRGGSVHLND
                     PDAGVVASSAILG"
BASE COUNT          303 a          707 c          728 g          341 t
ORIGIN      
        1 gtcgtacatc cacccgtgcc atgggcacgc gagccgcgtc cccgtgagct tgccctcgct
       61 gaggggaccg ccccggtggg cgcacacgtc accgcacgcg tacaccgcgc tgcccacccg
      121 ggccaggacg atccgggtgc cgtcgagctc caccagcttt ggttgaccgg ccggcacctc
      181 gtcgagcgag gccacccgcc gggccgagct cacgcgtctt ccttgaggac gaacgcccga
      241 tgcaggccgg tcatgtactg ccagcgcatc tcggtcgcct cgcgcaccgc ctcggcggcc
      301 tcggcctgct tctccggggt cgggctgtgc cgctcgacga tctgatagcc ccgctccccg
      361 tgcacctcgt cggcctcgat gtgaatcgcg aagaactcca cctcgtgatc gttgaacccg
      421 tagtgggtcc gcagcggagg aaggttgcgc ttgtagatgc cgggcacctg cgactcgagc
      481 ccgaccagga gcccggccgc cgcgacgtgg aagggcttgc gcgacatctc gtagcaccag
      541 gcggtgaggg cgcgcgtgct cggcagctgc cgggttccct cgacctcggc gcggctcacc
      601 ccgcacgcct cgccgaagcg gatgaggagg tccacgtgct tggtgccgga ctcctcctcg
      661 atgatgttct cgagcaggaa gtcccgggcg tccgggtcgg ggcagccgcc gtacacgatc
      721 gcgcaccacc gcgcgaactg cgagacgtac tggtagtgct ggcagaccca ctggccgagc
      781 tgggcgcggg tcagctctcc gttgacccac ttctcggtga acgggttcag ccggctgtgc
      841 cgggcgttga cggcgtgctc gagctgggtc cggaacgggc tcgcgttggt catggcgtcc
      901 ctccctgcgt gtttgaactc atgcgcgcgg atcggtctga gcgagcagcg cctgggtctg
      961 gctgtcgcga tacttgcgga gcgcggcgcg gatcggagga tgcttgccgg ccaggatggc
     1021 ccccgcgagc aggcccgcat tggtggcgcc ggcctgcccg atggccagcg tgccgaccgg
     1081 gatgcctgcc ggcatctgca ccgtcgagag cagcgagtcc atcccgttga gcgccttcga
     1141 ctgcatgggg acacccagca ccggcaggag ggtgcgcgcc gcgcacactc ccgcgaggtg
     1201 ggcggcgcct ccggctcccg cgatgatgac ctcgaggccg cgtccctccg ccgaggtcac
     1261 gtactcgtcg accagcgcgg gcgtgcggtg ggccgacatc acgttgacct cgtaagcgac
     1321 gccgagcgcg tccagcgcct tggccgcgtg ggccatcgtc tcccaatccg acttggagcc
     1381 catgaggatg cccaccagag gcgctgcgga tccggctgac atggcggcaa ttctagcaag
     1441 ggctcagctc gccgtggagc cgtttgcact gagccggccg tggggtgccc ttggcatggc
     1501 cgatgcaagt tgtgcgtgcc aagcagccgc ctgcatcgga agatccccgt gctggccggg
     1561 tggtcgatgc tcagcaagat gctatttgta ttggtgtttt agggttgcgg cggaccttct
     1621 tcggaccgcc tccgacccgc gatggcgagc gtgcgagtga ccaagagcgt cgtcagcgag
     1681 acgctgctcg taagcgtgag agtgcccatt cacgccgttg gcaggcgagg acgatgacga
     1741 cagagacgag cgagcaactg ctacgcgaaa tggtccggat ccgacggatc gaggagacgc
     1801 tggccgagct ctatccgtcg caggagatgc gcacgcccac ccacttctcc atcgggcagg
     1861 aggcggtggc ggtcggcgtg tgctcggtgc tgcggcggac cgacgcagtc tacagcggcc
     1921 atcggtgcca cgcacactat ctcgcgaagg gtggcgacct cggcggaatg gtcgccgagc
     1981 tctacggacg cgagaccgga tgcgcgcgcg gccgcggcgg ctccgttcac ctcaacgatc
     2041 ccgacgcggg tgtcgtcgcg tcgtcggcga ttctcgggc
//
LOCUS       DATMCV010000346         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_551346, whole genome shotgun sequence.
ACCESSION   DATMCV010000346 DATMCV010000000
VERSION     DATMCV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551346"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1735)
                     /locus_tag="VNU03_08095"
     CDS             complement(182..1735)
                     /locus_tag="VNU03_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74461.1"
                     /translation="MRRTHLGSWVVLGLTALALMAVGPAEAQKKTLVVALNQDPDLLD
                     PTLSRTYVGRIIFAHMCEKLFEIDENLRLSPQLAAALPAISDGGKTVTIKLRSGVKFN
                     DGTPMNAEAVKFSLDRHREMKGSNRRSELDPVTAVEVVDPLTVRLRLKSAFSPLVATL
                     ADRSGMPISPTAANKLGDKFATAPVCVGPWSFVERVPQDRIVLEKSTHYFDPGQAHFD
                     RIVFRVIPDDNVRLANLRSGDIDVMHLVAPTDSLNLKKEGRFEVASVTGLGYTGMTIN
                     LRNKTGKTQPPGDLGTPLANDPRVREALDLSLDREAINQVAWDGQYTPACTPIAPVSP
                     FYDKSRKCPGRDVAKAKQLLASAGLPNGYAFELTIVNDPQQRRFGEVIQGMAREAGFN
                     ITLKPSEFASALTDDDNGKLQAFAIGWSGRVDPDGNLHQFHTCGGSLNTTLVCDEAVD
                     ALLNKAREVSDQGQRAKLYREAIEKITARRNIVYLYHLNYIVAFPKNLKGYRAVPDGL
                     IRIKGVSWN"
     gene            1895..2068
                     /locus_tag="VNU03_08100"
     CDS             1895..2068
                     /locus_tag="VNU03_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74462.1"
                     /translation="MSENKFQQAELADLLGVIERLAGELSLAEEPARFAAALEDGAPA
                     AGGGAAPGSGSPR"
     gene            2080..>2259
                     /locus_tag="VNU03_08105"
     CDS             2080..>2259
                     /locus_tag="VNU03_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit A"
                     /protein_id="HWP74463.1"
                     /translation="MPDFPSLAAVAASIQQGKVSPVEVARHHLDRIARLDDRLRAFIT
                     VDADRALAAARRLEDD"
BASE COUNT          304 a          742 c          830 g          383 t
ORIGIN      
        1 gagcccgtac ttctggcgga tctcggcgag cccggccgcg tcggcgtcgg tgccgcccat
       61 gacgcgggcc gggtcgccgg ggatcatgcg gacgccggtg aagacgatca ggctgatcac
      121 gaacagggtc acgatcgaga tcgcgacccg ccggatcagg aagtcgagca ccggggctca
      181 cttagttcca ggacaccccc ttgatgcgga tcagcccgtc gggcaccgcc cggtaaccct
      241 tcaggttctt cgggaacgcg acgatgtagt tgaggtggta gagatagacg atgttgcgcc
      301 gcgcggtgat cttctcgatc gcctcgcggt agagcttcgc gcgctgcccc tggtcgctga
      361 cctcgcgcgc cttgttcagg agggcgtcga cggcctcgtc gcagaccagg gtggtgttca
      421 ggctgccgcc gcaggtgtgg aactggtgca ggttgccgtc gggatcgacg cgcccgctcc
      481 agccgatggc gaaggcctgg agcttgccgt tgtcgtcgtc ggtcagcgcg gaggcgaact
      541 cgctcggctt gagggtgatg ttgaagccgg cctcgcgggc catcccctgg atcacctcgc
      601 cgaagcggcg ctgctgcggg tcgttcacga tggtgagctc gaaggcgtag ccgttgggca
      661 gcccggccga ggcgaggagc tgcttggcct tggccacgtc gcggccgggg cacttgcggc
      721 tcttgtcgta gaacgggctc accggggcga tcggcgtgca ggcgggcgtg tactggccgt
      781 cccacgccac ctggttgatc gcctcgcggt cgaggctcag gtcgagggcc tcgcgcaccc
      841 gcgggtcgtt ggcgagcggg gtgcccaggt cgccgggcgg ctgggtcttg ccggtcttgt
      901 tccgcaggtt gatggtcatg ccggtgtagc cgaggccggt cacgctcgcc acctcgaagc
      961 gcccctcctt cttgaggttg agcgagtcgg tgggggccac caggtgcatg acgtcgatgt
     1021 cgcccgagcg caggttggcg aggcgcacgt tgtcgtcggg gatgacgcgg aacacgatcc
     1081 ggtcgaagtg ggcctggccg ggatcgaagt agtgggtcga cttctcgagc acgatgcggt
     1141 cctgcggcac gcgctccacg aaggaccacg gtcccacgca caccggggcg gtggcgaact
     1201 tgtcgccgag cttgttggcc gcggtaggcg agatcggcat gccggagcgg tcggcgaggg
     1261 tggccaccag cggggagaag gcgctcttca gccggagccg gacggtgagc ggatccacca
     1321 cctcgaccgc ggtcaccggg tccagctcgc tgcgccggtt cgagcccttc atctcgcggt
     1381 gccgatcgag ggagaacttc accgcctccg cgttcatcgg ggtcccgtcg ttgaacttca
     1441 ccccgctgcg cagcttgatg gtcaccgtct tgccgccgtc ggagatcgcg ggcagcgcgg
     1501 cggcgagctg gggggagagg cgcaggttct cgtcgatctc gaagagcttc tcgcacatgt
     1561 gggcgaagat gatccggccc acgtaggtgc gcgacagggt cgggtccagc aggtcgggat
     1621 cctggttgag cgctaccacg agggtcttct tctgggcctc ggccggcccc accgccatca
     1681 gggccagggc cgtgagcccg agcacgaccc acgatccaag gtgcgtcctg cgcatggtct
     1741 tctcctttct gaaagcgggt aaccagccgg ctgggctcgc ggttcccctc gcggttcccc
     1801 tgcggtttcc gaagtcgacc gcctgagcgt gagtaccctc gggggcctcg tgttgtcaag
     1861 gattgtactc gggcccgcgt agtatgctcc cgccatgagt gagaacaagt tccagcaggc
     1921 ggagctggcc gacctcctgg gggtgatcga gcggctggcc ggcgagctgt cgctggccga
     1981 ggagcccgcg cgcttcgcgg ccgcgctgga ggacggcgcg ccggccgccg gcggcggcgc
     2041 cgcgcccggc tccgggtctc cccgctaacc ggcgcggcgt tgcccgactt cccgagcctg
     2101 gccgcggtcg ccgcgtcgat ccagcagggc aaggtctccc cggtcgaggt cgcgcgccac
     2161 cacctcgatc ggatcgcacg gctcgacgat cgcctgcgcg ccttcatcac ggtggacgcc
     2221 gatcgcgcgc tggccgcggc ccgccgcctc gaggacgac
//
LOCUS       DATMCV010000347         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_351797, whole genome shotgun sequence.
ACCESSION   DATMCV010000347 DATMCV010000000
VERSION     DATMCV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351797"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..675
                     /locus_tag="VNU03_08110"
     CDS             157..675
                     /locus_tag="VNU03_08110"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP74464.1"
                     /translation="MIGSVAEFTRYWAGVRRRTWAAVDRVEPARLDWAPRPGEMTCGE
                     IVRHLAGAERFFVTKVVEDRFTDDLTPGAGLDHAQTRALLERVHREEMGRLAALDDGV
                     LASPRRDLDGGTIKAWRFLMAMVEHEVHHRSQLDCYLAEMGVDAPQIFGRRFEDVAAQ
                     VRAEQAPAGDPR"
     gene            672..1085
                     /locus_tag="VNU03_08115"
     CDS             672..1085
                     /locus_tag="VNU03_08115"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HWP74465.1"
                     /translation="MTDCIFCKIRDGQVPSMKIYEDERTLAFMDIHPLSPGHCLVIPK
                     AHAATLFEVEPDDLQAAVATAQRVALAIREGLAPDGLNMLQANGAAAFQSVSHFHLHL
                     IPRWANDGKGFDWKLVPGHRDQIMKAGERIRALLR"
     gene            1100..1504
                     /locus_tag="VNU03_08120"
     CDS             1100..1504
                     /locus_tag="VNU03_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWP74466.1"
                     /translation="MAERKIRVLVAKPGLDGHDRGAKIVARALRDAGFEVIYTGLHQT
                     PEQIVATAVQEDVDAIGLSVLSGAHNYLFGRVLDLLKEKGAADIAVFGGGIIPQDDIQ
                     ALKALGVKELFTPGTSTQDIIRFVRDNIRAAV"
     gene            1521..>2171
                     /locus_tag="VNU03_08125"
     CDS             1521..>2171
                     /locus_tag="VNU03_08125"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP74467.1"
                     /translation="MAQPDNREAILDQFTRQAVPFSTAPGIRDEEALRLLVTASGAGP
                     ADTVLDVACGPGLVAAAFARVARHVTGIDLTPAMIDRAQALVSERGLANVTLQVGDVR
                     PLPYADRAFALVVSRFAFHHFPDPGAVLREMARVCAPGGRVVVADAAVSPDPARAAAF
                     NRMERLRDPSHVRALSLAELAGLFEAAGLPMPAVTRCEVRAEVEGLLSRSFPAPGDA"
BASE COUNT          308 a          776 c          786 g          301 t
ORIGIN      
        1 gcgagctcgc ctacaagggc aagaagatgg tgatcaacgg gggcaagatc gggccgctca
       61 cccagaagct ctacgacgcc atcgtggcca tccagtacgg caaggcaccg gacccggacg
      121 gctggacgct cgcgatttga ggtcgcgagg ccgtcgatga tcggctcggt cgcggagttc
      181 acccggtact gggccggcgt ccgccgccgg acctgggccg cggtggaccg cgtcgagccg
      241 gcccggctcg actgggcccc gcggccgggc gagatgacct gcggggagat cgtgcggcac
      301 ctcgcggggg cggagcggtt cttcgtgacc aaggtggtcg aggaccggtt caccgacgac
      361 ctgaccccgg gggcgggcct cgatcacgcg cagacccgag ccctgctcga gcgcgtgcat
      421 cgagaggaga tgggtcggct ggccgcgctc gacgacgggg tgctggcgag cccgcgccga
      481 gacctcgacg gtggcaccat caaggcctgg cgattcctga tggcgatggt ggagcacgag
      541 gtgcaccatc ggagccagct cgactgctac ctggccgaga tgggagtgga cgcgccccag
      601 atcttcggcc ggcggttcga ggacgtggcg gcgcaggtga gggcggagca ggccccggcc
      661 ggagacccgc gatgaccgac tgcatcttct gcaagatccg cgacgggcaa gtcccctcga
      721 tgaagatcta cgaggacgag cgcaccctcg ccttcatgga catccacccc ctgagcccgg
      781 gccactgcct ggtgatcccg aaggcgcacg cggccaccct gttcgaggtg gagcccgacg
      841 acctgcaggc cgcggtcgcc accgcccagc gggtggcgct ggcgatccgc gaggggctcg
      901 ccccggacgg gctcaacatg ctgcaggcca acggcgccgc cgccttccag tcggtgtcgc
      961 acttccatct gcacctcatc ccgcgctggg ccaacgacgg caagggcttc gactggaagc
     1021 tcgtgcccgg tcaccgggac cagatcatga aggcgggcga gcgcattcgc gcgctcctgc
     1081 gctgaacggg ggaccgacca tggccgagcg aaagatccgc gtgctggtgg cgaagcccgg
     1141 tctcgatggg cacgaccgcg gcgcgaagat cgtggcgcgg gccctgcggg acgcgggctt
     1201 cgaggtgatc tacaccggcc tgcaccagac ccccgagcag atcgtggcca ccgcggtgca
     1261 ggaggacgtc gacgcgatcg ggctctcggt gctctcgggc gcccacaact acctcttcgg
     1321 ccgcgtgctc gacctgttga aggagaaggg cgccgccgac atcgcggtct tcggcggcgg
     1381 catcatcccc caggacgaca tccaggcgct caaggcgctc ggggtgaagg agctgttcac
     1441 ccccgggacc tcgacccagg acatcatccg gttcgtccgc gacaatatcc gggcggcggt
     1501 ctagccgcgc gcgtcacccc atggctcagc cggacaaccg cgaggcgatc ctcgatcagt
     1561 tcacccggca ggcggtgccg ttctcgaccg cgcccggcat ccgcgacgag gaggccctcc
     1621 gtctgctggt caccgcaagc ggggcggggc cggccgacac cgtgctcgac gtggcctgcg
     1681 gccccgggct cgtggcggcg gccttcgcgc gggtcgcccg tcacgtcacc ggcatcgatc
     1741 tcacccccgc gatgatcgac cgcgcccagg ccctcgtctc cgagcgcggg ctcgccaacg
     1801 tgaccctgca ggtcggcgac gtccggccgc tgccctacgc cgatcgggcc ttcgccctcg
     1861 tggtctcccg gttcgccttc caccacttcc cggatcccgg ggcggtcctg cgggagatgg
     1921 cgcgggtgtg cgctccgggc ggccgcgtgg tggtggccga cgccgcggtc tcgcccgatc
     1981 cggcgcgggc ggccgcgttc aaccggatgg agcggctgcg cgatccctcc cacgtgcgcg
     2041 ccctgtcgct ggccgaactg gccgggctct tcgaggcggc cgggctgccc atgcccgcgg
     2101 tcacgcgctg cgaggtacgg gcggaggtgg aggggctgct gtcgcgctcg ttcccggccc
     2161 cgggcgacgc c
//
LOCUS       DATMCV010000348         2050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_286798, whole genome shotgun sequence.
ACCESSION   DATMCV010000348 DATMCV010000000
VERSION     DATMCV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286798"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VNU03_08130"
     CDS             complement(<1..988)
                     /locus_tag="VNU03_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP74468.1"
                     /translation="MTATALELEVVGLTKRFGGFTALDDVSLRVAAGSFHALLGENGA
                     GKSTLVKCVVGYQRPDAGVVLVGRRERDIRSPADAHGLGVGMVYQHFTLVPSMTVAEN
                     LVMARDDVPLRVQWRRERERLAAFMTTVPFALDLDRPVWQLAAGEKQKVEILKLLYLR
                     RRLVILDEPTSVLTPAEADEVLGMLQAMARAGQVTVLMITHKFREVMAFADAVSVLRR
                     GRLAGEGRVAELTPARLAEMMVGAREIPEAHAERMNGWDPGRAARLVIDGLVVEDEAG
                     LVAVDRVSLAVRPGEIVGIAGVSGNGQDEFFAALSGERLSPEAGNVVVDGTGV"
     gene            complement(985..1677)
                     /locus_tag="VNU03_08135"
     CDS             complement(985..1677)
                     /locus_tag="VNU03_08135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWP74469.1"
                     /translation="MTQRFVVADPYPWPYDGDLRPGNTALLVIDMQTDFCGVGGYVDV
                     MGYDLALTRSPIKPIQAVLEAARAAGFHVIHTREGHRPDLSDLPANKRWRSRRIGAGI
                     GDPGPCGRILVRGEPGWEIIPELAPAPGEAVIDKPGKGSFCATDLELILRTRGITNLV
                     LTGITTDVCVHTTMREANDRGFECLLLEDCTGATDAGNHRAAVKMVTMQGGVFGAVAR
                     SEALLAALERRA"
     gene            complement(1715..>2050)
                     /locus_tag="VNU03_08140"
     CDS             complement(1715..>2050)
                     /locus_tag="VNU03_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74470.1"
                     /translation="TALGGFLAGVGGSSLSLYYPGSWTEGLSSGQGLMAVALVIFARW
                     DPLRCFYAALLFGGASALGPALQSIGISGGYYLFNAAPYVVTLGIMIATCSPRRTLAG
                     VPGELSINR"
BASE COUNT          307 a          737 c          744 g          262 t
ORIGIN      
        1 cgaccccggt gccgtcgacc accacattgc cggcttcggg ggagagccgc tcgcccgaca
       61 acgcggcgaa aaattcgtcc tggccgttgc cggagacgcc ggcgatgccc acgatctctc
      121 ccgggcgcac cgcgagggag acgcggtcca ccgcgacgag cccggcctcg tcctcgacca
      181 cgagcccgtc gatcaccagg cgggccgccc ggcccggatc ccacccgttc atgcgttcgg
      241 cgtgggcctc ggggatctcc cgcgcgccca ccatcatctc ggccaggcgt gcgggcgtca
      301 gctcggccac ccgcccctca ccggcgagcc ggccccggcg cagcaccgac accgcgtcgg
      361 cgaacgccat gacctcgcgg aacttgtggg tgatcatgag cacggtcacc tggccggccc
      421 gggccatggc ctggagcatg ccgagcacct cgtcggcctc ggcgggcgtg aggacggagg
      481 taggctcgtc gaggatgacg aggcgccggc ggaggtacag cagcttgagg atctcgacct
      541 tctgcttctc gccggcggcg agctgccaca ccggccggtc gagatcgagg gcgaacggga
      601 cggtggtcat gaaggcggcg agccgctcgc gctcacgccg ccactggacg cggagcggca
      661 cgtcgtcgcg cgccatcacc aggttctccg ccaccgtcat gctcggcacc agcgtgaagt
      721 gctggtagac catgccgacg ccgaggccgt gcgcgtcggc cggggagcgg atgtcccgtt
      781 cccggcgccc gacgaggacc acgcccgcgt cgggccgctg gtagccgacc acgcacttca
      841 cgagggtgct cttgcccgcg ccgttctccc cgagaagggc gtggaacgat cccgcggcca
      901 cgcggagcga gacgtcgtcg agggcggtga agcccccgaa gcgcttggtg aggccgacca
      961 cctcgagctc gagggcggtc gcggtcacgc gcggcgctcg agggcggcga gcaaggcctc
     1021 ggagcgggcc accgcgccga acacgccgcc ctgcatcgtg accatcttga ccgcggcgcg
     1081 gtgattgccc gcgtcggtgg cccccgtgca gtcctcgagg agcaggcact cgaagccgcg
     1141 gtcgttggcc tcgcgcatgg tcgtgtgcac gcacacgtcc gtggtgatgc cggtgagcac
     1201 gaggttcgtg ataccgcggg tgcggaggat cagctcgagg tcggtggcgc agaacgagcc
     1261 cttgccgggc ttgtcgatga ccgcctcgcc cggggcgggc gccagctcgg ggatgatctc
     1321 ccacccgggc tccccgcgga cgaggatgcg gccgcagggg ccggggtcgc cgatgccggc
     1381 gccgatgcgg cgggaccgcc accgcttgtt ggcgggcagg tcggacaggt ccggccggtg
     1441 cccttcccgc gtgtggatca cgtgaaagcc cgccgcgcgc gcggcctcga gcacggcctg
     1501 gatcggcttg atgggcgagc gcgtgagcgc gaggtcgtag cccatcacgt cgacgtagcc
     1561 gccgacgccg cagaaatcgg tctgcatgtc gatgaccagg agggcggtgt tgcccggacg
     1621 gagatcgccg tcgtacggcc acggatacgg gtcggccacc acgaaacgct gcgtcatcgt
     1681 cgtcacctcg ctgcgctcac gcgccggggg cgcgctagcg gttgatgctc agctcgccgg
     1741 ggacgcccgc gagcgtccgg cgcggcgagc acgtcgcgat catgatcccg agcgtcacca
     1801 cgtacggcgc ggcgttgaac agatagtagc cgccgctgat cccgatcgac tggagcgccg
     1861 ggcccagcgc gctcgcgcca ccgaacagga gcgcggcgta gaagcagcgg aggggatccc
     1921 agcgcgcgaa gatgaccagc gccaccgcca tcaacccctg cccgctcgac aggccctccg
     1981 tccagctccc gggatagtac aacgacaggg acgagccacc cacgccggcg aggaagccgc
     2041 cgagggcggt
//
LOCUS       DATMCV010000349         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_154311, whole genome shotgun sequence.
ACCESSION   DATMCV010000349 DATMCV010000000
VERSION     DATMCV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154311"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /locus_tag="VNU03_08145"
     CDS             <1..1662
                     /locus_tag="VNU03_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWP74471.1"
                     /translation="VEYLFTNPGSAETGLFAAIAEDGDNRLVVGKHEGLVAAMADGYH
                     RVSGKVGVVIAHVMGGSYQLAGQLFNAQVAGSSLLVIAGDWASELQDYRGLAPFPGLS
                     QAESMRPITKEARCAYQVHTNPKAISVATIRALREATTPPTGPVYLSISAELLHTEGL
                     EAVVGEGANYQIERPGPARAKTVEAIAKRLGEARCPVLMFGDDVWREGAQAEAVRLAE
                     ALEAPVFASRQIFPNFPTRHPLFCGNYPVSKDFTKTTGLEPDLIFLVGCQGVHGSVNE
                     PVIMQIGPNPLLMGRHYPLDVAAQCELPETLKAITQALGRVPGADKGPKQRSKVRAFA
                     AMLIEREEQLVREHEHDTPVHPAVLEAHLASVLPKNTVMVQESSTARTALMAFGHGGM
                     DWTRSGGGSLGFGVGAAIGAKVAVGRDRPVVLHLGDGALGYSASGFWTIARYNTAILT
                     IVSNNESYQIVRHNWAKEMPDSKMIREGKYPGLWLSAPATDHVALARSQGVDGEAVTS
                     VKELESALRRGMDKITRENRPYLIDVSVQREGIGAESTWYQDWQL"
     gene            complement(1694..>2078)
                     /locus_tag="VNU03_08150"
     CDS             complement(1694..>2078)
                     /locus_tag="VNU03_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74472.1"
                     /translation="YYFLVTRSHGDPHVESMNVGVSPHAMTRPEHDDERRRVQERLQA
                     DGWKPGRFVYRTPEQQALHGGKTSEAGRTYWLKGEALLHLEPKRVDEEQRGEDEATAG
                     RFMLAVSIFERTASSTYPRLDFTAP"
BASE COUNT          310 a          700 c          775 g          293 t
ORIGIN      
        1 gtggagtacc tgttcaccaa cccgggctcc gcggagacgg gtctcttcgc cgccatcgcg
       61 gaggatgggg acaaccggtt ggtcgtcggc aagcacgagg gcctggtggc cgcgatggcg
      121 gacggctacc accgggtcag cggcaaggtg ggcgtggtca tcgcgcacgt catgggtggg
      181 tcctaccagc tcgccggcca gctcttcaac gcgcaggtgg cgggctcctc gctgctggtg
      241 atcgcgggcg actgggcctc ggagctgcag gattatcgcg gcctcgcccc gttccccggg
      301 ctgagccagg cggagtcgat gcggcccatc accaaggaag cgcggtgcgc ctaccaggtg
      361 cacaccaacc cgaaggcgat cagcgtggcg acgattcgcg cgctgcgcga ggcgaccacg
      421 ccgcccaccg ggccggtgta cctctcgatc agcgcggagc tgctgcacac ggaagggctc
      481 gaggcggtcg tcggtgaggg cgcgaactac cagatcgagc ggccggggcc ggcgcgggcg
      541 aagacggtgg aggcgatcgc caagcggctc ggtgaggcgc gctgcccggt gctgatgttc
      601 ggcgacgacg tgtggcgcga gggcgcgcag gcggaggcgg tgcggctggc cgaggcgctg
      661 gaggcgccgg tgttcgcgag ccggcagatc ttcccgaact tcccgacgcg gcatccgctc
      721 ttctgcggga actacccggt gtcgaaggac ttcaccaaga cgaccgggct cgagccggac
      781 ctgatcttcc tggtgggctg ccagggcgtg cacggcagcg tgaacgagcc cgtcatcatg
      841 cagatcgggc cgaacccgct cctgatgggc cggcactacc cgctcgacgt ggccgcgcag
      901 tgcgagctgc ccgagacgct caaggccatc acccaggcgc tcggccgggt gcccggggcc
      961 gacaaggggc cgaagcagcg ctcgaaggtg cgcgcgttcg cggccatgct gatcgagcgg
     1021 gaggagcagc tcgtgcgcga gcacgagcac gacacgccgg tgcaccccgc ggtgctggag
     1081 gcgcacctgg cgagcgtact gccgaagaac acggtgatgg tgcaggagag ctcgaccgcg
     1141 cgcaccgcgc tgatggcgtt cggccacggc gggatggact ggacgcggag cggcggcggc
     1201 tcgctcggct tcggggtcgg cgcggcgatc ggcgcgaagg tcgcggtggg gcgcgaccgg
     1261 ccggtcgtcc tgcatctcgg cgacggggcg ctcggctaca gcgcctcggg tttctggacg
     1321 atcgcgcgct acaacaccgc gatcctcacc atcgtgtcca acaacgagag ctaccagatc
     1381 gtgcgccaca actgggccaa ggagatgccg gactccaaga tgatccgcga gggcaagtat
     1441 cccggcctct ggctcagcgc ccccgccacc gatcacgtgg cgctggcgcg ctcgcagggt
     1501 gtggacggcg aggcggtgac ctcggtgaag gagctcgagt cggcgctccg ccgcggcatg
     1561 gacaagatca cccgcgagaa ccggccctac ctgatcgacg tgtcggtcca gcgcgagggg
     1621 atcggcgccg agtcgacctg gtaccaggac tggcagctct agcccaggcg gcgcgtctct
     1681 aggtaggcgc gcgtcagggc gcggtgaagt cgagccgcgg gtaggtgctg gacgcggtgc
     1741 gctcgaagat gctcaccgcg agcatgaagc gcccggccgt cgcctcatcc tcgcctcgct
     1801 gctcctcgtc cacccgcttc ggctccaggt ggagcagcgc ctcgcccttg agccagtagg
     1861 tccggccggc ctccgacgtc ttgccgccgt gcagcgcctg ctgctccggc gtgcggtaga
     1921 cgaagcgccc gggcttccac ccgtcggcct gcaggcgctc ctgcacccgc cggcgctcgt
     1981 cgtcgtgctc cggccgggtc atcgcgtgcg gcgagacgcc cacgttcatg gactccacgt
     2041 gcgggtcgcc gtgcgagcgc gtcacgagga agtaatag
//
LOCUS       DATMCV010000350         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_286874, whole genome shotgun sequence.
ACCESSION   DATMCV010000350 DATMCV010000000
VERSION     DATMCV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286874"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1185
                     /locus_tag="VNU03_08155"
     CDS             94..1185
                     /locus_tag="VNU03_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74473.1"
                     /translation="MSAASPAAKVFRLKGDYSAINRHYQGQGWTDGLPIVPPTEDDVR
                     EFLRFTDRGPREVVAVLPPRQGEATVERITINAVMAGCRPEYLPIVITAVEALADPAF
                     NLDSIQATTHPVAPLIIVNGPIAKEVGINSGYNAFGQGFRANLTIGRAIRLLLMNVGG
                     GLPGTGDRATQGTPAKIAYCVAENEGANPWEPLHVEHGFPADTSVVTAIACEGPHNIQ
                     DHFSYTGLGVLKVIAGAMGQAGSNNILAGGHPLLSLGPEHAATIARDGFTKRQVKEFL
                     VEHARFPLARLGDEYRAHLIQRGAKDAPDTMVPAVHSVDDVTVIVTGGAGKHSCWQPT
                     FGDQTWPARRAIARRDGTPLKAIAELARS"
     gene            1225..2538
                     /locus_tag="VNU03_08160"
     CDS             1225..2538
                     /locus_tag="VNU03_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HWP74474.1"
                     /translation="MRSHSIRGFLVALVLLVLAWATPALADETCNSPYMGNLIKAQED
                     YVYVWTLGMKGVGDGSDKLVTIDANPSSARYGKVIHKVSVGGRGEAHHMGFTDDRRFL
                     WAGGLDDSRIVVFDVGTDPARPRIVKRITDMPKRTGYVGPHTFYALPGRMLVQGLSNS
                     KDKGGVTGMALYSNQGELVTTYAMPTAEGGDGYGYDIAINPRKNVLLTSSFTGWNNYM
                     MELGKLVANPEAMKRFGSTMVIWDLKAFTPKKVLAVPGAPLEIRWSLKEGDNWAITAT
                     ALTSKLWLVKPDANGEWQAKEVATIGDPAKIPLPVDISIRNDGTGLWVNTFMDGKTRY
                     FDLSNPEQPRQTYEKVTGKQVNMISQSWDGTRVYISSSLLANWDKAGADNEQFVRAFK
                     WDGKELIPAFEVDFNKEKLGRAHHMKFGSRAMKAAYRDAPLASAR"
     gene            2543..>2938
                     /locus_tag="VNU03_08165"
     CDS             2543..>2938
                     /locus_tag="VNU03_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74475.1"
                     /translation="MPRPLLPTAAWLVVGLALRGLALAGPAWGHGDEARDQPPPVKAD
                     FVPPVPGTYTLHSIMRAPGGQVVDLDGRDRRLAEYTTGKITLLSFVYTRCRDAWGCPL
                     AYRVFDAVGAAVERSPDLRSRVRLVTLSFD"
BASE COUNT          509 a         1045 c          978 g          406 t
ORIGIN      
        1 catcccccac ccgctggggg gcatccgcga ggacgaggtc atgaagaagg cggagctggt
       61 ggtcgaggtc gtccggaagg cggtcacgcg gggatgagcg cggccagccc ggcggcgaag
      121 gtcttccgcc tcaagggcga ctactctgcc atcaaccggc actaccaggg gcagggctgg
      181 accgacggcc tgccgatcgt gccgccgacg gaagacgacg tgcgcgagtt cctgcgcttc
      241 accgatcgtg ggcctcgcga ggtggtggcg gtactgccgc cgcggcaggg cgaggccacg
      301 gtggagcgca tcacgatcaa cgcggtgatg gcggggtgcc ggccggaata ccttccaatt
      361 gttattacgg ccgtcgaggc gctagccgat cccgcgttca acctcgactc gatccaggcc
      421 accacgcacc cggtggcccc gctgatcatc gtcaacgggc cgatcgcgaa ggaggtcggg
      481 atcaactcgg ggtacaacgc gttcggccag ggcttccgcg ccaacctcac catcggacgc
      541 gcgatccggc tgctcctgat gaacgtgggc ggcggcctgc ccggcaccgg cgaccgcgcc
      601 acccagggca ccccggcgaa gatcgcctac tgcgtggccg agaacgaggg cgcgaatccg
      661 tgggagcctc tccacgtcga gcacggcttc ccggccgaca cgagcgtggt caccgcgatc
      721 gcctgcgagg ggccgcacaa catccaggac cacttcagct acaccgggct cggcgtcctc
      781 aaggtgatcg caggggcaat gggccaggcg ggcagcaaca acatcctggc cggcggccat
      841 cccctgctct cgctcgggcc cgagcacgcc gccaccatcg cacgcgacgg cttcaccaag
      901 cggcaggtga aggagttcct ggtcgagcac gcgcgcttcc ccctcgcccg cctcggcgac
      961 gagtatcgcg cgcatctgat ccagcgcggc gcgaaggacg cgcccgacac catggtgccc
     1021 gcggtccact cggtcgacga cgtgaccgtg atcgtgaccg gcggcgcggg caagcattcc
     1081 tgctggcaac ccaccttcgg cgatcagacg tggccggccc gtcgggccat cgcacggaga
     1141 gacggcaccc cgctcaaggc aatcgcggag ctggcccgca gctagcacgc ggcacacccg
     1201 actcacctag aaggaggctc aaccatgcgc tcacactcta ttcgcggatt cctcgtcgcg
     1261 ctcgttctgc tggtcctggc ctgggcgact ccggccctcg cggacgagac gtgcaactcc
     1321 ccctacatgg gcaatctgat caaggcccag gaagactacg tctacgtgtg gacgctcggg
     1381 atgaagggcg tcggcgacgg ctcggacaag ctcgtcacca tcgacgcgaa cccctcgtcg
     1441 gcgcgctacg ggaaggtgat ccacaaggtc tcggtcggcg ggcgggggga agcgcaccac
     1501 atgggcttca ccgacgaccg gcgcttcctc tgggcgggcg ggctcgacga cagccggatc
     1561 gtcgtcttcg acgtgggcac cgacccggcc cggccgcgaa tcgtcaagcg catcaccgac
     1621 atgccgaagc gcaccggcta cgtcgggccc cacaccttct acgccctgcc gggacggatg
     1681 ctggtgcagg gtctgtccaa cagcaaggac aagggcgggg tcaccggcat ggccctctac
     1741 agcaaccagg gtgaactggt caccacctac gcgatgccga ccgcggaggg cggggatggc
     1801 tacggctacg acatcgcgat caacccgcgg aagaacgtgc tgctgacctc gagcttcacc
     1861 gggtggaaca actacatgat ggagctgggc aaactcgtgg ccaaccccga ggccatgaag
     1921 cgcttcggca gcaccatggt gatctgggac ctcaaggcgt tcacgcccaa gaaggtgctc
     1981 gcggtcccgg gggcgccgct cgagatccgc tggtcgctga aggagggcga caactgggcg
     2041 atcaccgcga ccgcgctcac ctcgaagctc tggctcgtga agccggacgc gaacggggag
     2101 tggcaggcca aggaggtggc caccatcggc gacccggcca agatcccgct cccggtcgac
     2161 atcagcatcc gcaacgacgg caccgggctc tgggtgaaca ccttcatgga cggcaagacc
     2221 cgctacttcg acctgagcaa ccccgagcag ccccggcaga cctacgagaa ggtcaccggc
     2281 aagcaggtca acatgatctc ccagagctgg gacggcaccc gcgtctacat ctcgtcctcc
     2341 ctgctcgcca actgggacaa ggcgggcgcg gacaacgagc aattcgtgcg cgcgttcaag
     2401 tgggacggca aggagctgat ccccgcgttc gaggtggact tcaacaagga gaagctcggc
     2461 cgcgcgcacc acatgaagtt cggcagccgg gccatgaagg ccgcctaccg ggacgcgccc
     2521 ctggccagtg cgcggtagct cgatgccgcg cccgctcctc cccaccgcgg cgtggctcgt
     2581 cgtgggcctg gccctcaggg gcctggccct cgccgggccg gcctggggcc acggcgacga
     2641 ggcgcgcgat cagccgccgc cggtgaaggc cgacttcgtc ccgcccgtcc ccggcaccta
     2701 caccctgcat tccatcatgc gggctcccgg cggccaggtg gtcgacctcg acggccgcga
     2761 ccggcggctc gccgagtaca ccaccggcaa gatcacactg ctgagcttcg tctacacgcg
     2821 ctgccgcgac gcgtggggct gtcccctcgc ctatcgggtc ttcgacgcgg tgggcgccgc
     2881 ggtcgagcgc tccccggacc tccgctcgcg cgtgcggctg gtcacgctct ccttcgat
//
LOCUS       DATMCV010000351         5197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_86407, whole genome shotgun sequence.
ACCESSION   DATMCV010000351 DATMCV010000000
VERSION     DATMCV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86407"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VNU03_08170"
     CDS             complement(<1..916)
                     /locus_tag="VNU03_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWP74476.1"
                     /translation="MRRWPFQSSEQAPLEHFPIIEDLLRRNIEDIFRVERQEARGVTF
                     GWGGTLLVEPARALALIEPRFKSFGYTPFLGRQGDLAWIRAVPLAEVSERTKAGVNLV
                     LFALTVLSTLAAGSLVSGSFPFVTFNPSLEPFRLLDGVPFAFTLLAILGTHEFGHYFT
                     ARYHGASVSLPYFIPAPPPIFLFGTMGAVIRMRSPARDRNSLFDIAVAGPLAGLVVAV
                     PALLLGLSWSRVGMVLPDHTGLTFGDSLLMRALTWLVFGSIPPGMDVFVHPVALAGWV
                     GLFVTALNLFPVGQLDGGRIAYALFGPWH"
     gene            complement(926..1375)
                     /locus_tag="VNU03_08175"
     CDS             complement(926..1375)
                     /locus_tag="VNU03_08175"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HWP74477.1"
                     /translation="MTAAADPRDALGGRLGHTFRDPDLLREALTHRSFANEIAGSRDN
                     ERLALLGDAMLSLVVAERLLAIAPADPVGVLTPRRAALVSGENLARWAAALDLGVHLR
                     LGRGEEQMGGRAKESVLATALEAVLGVVYLEGGLDAGRRAIALLAVW"
     gene            complement(1376..2629)
                     /gene="fabF"
                     /locus_tag="VNU03_08180"
     CDS             complement(1376..2629)
                     /gene="fabF"
                     /locus_tag="VNU03_08180"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWP74478.1"
                     /translation="MKTVELRRVVITGVGAVTPVGNTAEEFWAALIQGKSGIGPITRF
                     DASPLPTKIAGELKGFDPLRYMDKKDDRKLDPFLKYAIACAAMAVEDAGLNVEKVDRT
                     RFGVLVGSGIGGITTLLETHKTLLEKGPDRVSPFFIPMLIVNMASGLISMRFGAKGPN
                     SSIVTACATGNHAIGDAMKIIQRNDADIMIAGGSEAIILPLTFAGFCQMKAMSTRNDD
                     PGRASRPFDATRDGFVCGEGGGLLVLESLDHALGRDARIYAEVVGYGMTGDAHHMTAP
                     DPEADGAARAMALAMKDAEVEPSAVGYINAHGTSTPYNDKSETMAIKRVFGEHARKLA
                     VSSTKSMTGHLLGAAGGIEAIATALAIHHGILPPTINYETPDPDCDLDYVPNQARKQD
                     VEVALSNAFGFGGTNATIVLRKYRP"
     gene            complement(2644..2880)
                     /locus_tag="VNU03_08185"
     CDS             complement(2644..2880)
                     /locus_tag="VNU03_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWP74479.1"
                     /translation="MAKPVEERVKEIIVEQLGVEEDEVLPAAKFIEDLGADSLDTVEL
                     VMAFEEEFDLEIPDEDAEKIATVGDAISYIKENQ"
     gene            complement(2917..3669)
                     /gene="fabG"
                     /locus_tag="VNU03_08190"
     CDS             complement(2917..3669)
                     /gene="fabG"
                     /locus_tag="VNU03_08190"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003394969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HWP74480.1"
                     /translation="MSEGKTLAGKVAIVTGGTRGIGLAIARLLAEDGASVVVSGRDAA
                     RLEAAVKELESLGAPAMAVAADAAKREDADRLVEATREGFGRIDVLVNNAGITRDQLL
                     VRMKDDDWDQVLDTNLRGVFLMTRAVGKVMMRQKSGRIINIASTAGAMGNPGQVNYSA
                     AKAGVIGLTKAAGRELAHWNILVNAVAPGLIETDMAASIPAEAREAMLQQVPLKRIGQ
                     GREVAEVVRFLAGDGASYITGQTIHVNGGLYV"
     gene            complement(3666..4616)
                     /gene="fabD"
                     /locus_tag="VNU03_08195"
     CDS             complement(3666..4616)
                     /gene="fabD"
                     /locus_tag="VNU03_08195"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWP74481.1"
                     /translation="MVAGPIAFLFPGQGSQEVGMGRALAEASPGARAVWAEADAALGF
                     PLSRLCFEGPAEELALTANTQPAVLTASVAAAAALAERGVTPDLAAGHSLGEYSALVV
                     GGALSFKDAVRLVRRRGEFMQEAVPVGTGAMAALMGVDLPTVEQVCAEAAQGEVVEVA
                     NINSPGQIVVAGHRTAVERAVAGAGARGGKKSVMLPVSAPFHCALMKPAADRLAAELD
                     RVAVSAPRIRIVRNVDGGVTISADEVKPFLVQQVASPVRWTDCTQRLSREGATAFLEV
                     GPGRVLTGLLKRILDGVRGHAVEDPASLEKAVAALAGGEP"
     gene            complement(4610..>5197)
                     /locus_tag="VNU03_08200"
     CDS             complement(4610..>5197)
                     /locus_tag="VNU03_08200"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HWP74482.1"
                     /translation="YRTVLCIGAECLSRITDFTDRGTCILLADAAGAVVLRPSDGDAG
                     ILDTDLYSDGRYGELLIQPAGGSRHPATHETVDKRMHFARMKGNEVFKVAVRMFGESA
                     ERILKNNGFTAADLDLFVPHQANLRIIEAAVKRLKVPMERVMVNVERYGNTGAASVYV
                     ALEEAWTNGRVKPGDLILLAAFGGGFTWGAALLRW"
BASE COUNT          757 a         1918 c         1774 g          748 t
ORIGIN      
        1 ggtgccaggg cccgaacagc gcgtaggcga tgcggccgcc gtcgagctgc cccaccggga
       61 acaggttgag ggcggtcacg aagagcccga cccagccggc cagggccacc ggatggacga
      121 acacgtccat gccgggcggg atcgatccga agaccagcca ggtgagcgcg cgcatcagca
      181 gcgagtcccc gaaggtgagc ccggtgtggt ccggcagcac catgcccacc cgggaccacg
      241 agagccccag caggagcgcg ggcaccgcga ccaccagtcc ggccagcggc cccgcgaccg
      301 cgatgtcgaa gagcgagttg cggtcgcgcg cgggcgagcg catgcggatc accgccccca
      361 tggtgccgaa gaggaagatc ggcggcgggg ccgggatgaa gtagggcagg ctcaccgagg
      421 cgccgtggta gcgggcggtg aagtagtggc cgaactcgtg ggtgccgagg atcgcgagaa
      481 gggtgaacgc gaacgggacg ccgtcgagca gccggaacgg ctccagggag gggttgaagg
      541 tcacgaacgg gaacgagccg ctcaccaggc tgcccgcggc cagggtggag agcacggtga
      601 gcgcgaacag gaccaggttg acgcccgcct tcgtccgctc gctcacctcg gcgagcggca
      661 ccgcgcggat ccaggccaga tccccctgcc gccccaggaa cggggtgtag ccgaaggact
      721 tgaaccgcgg ctcgatcagg gcgagcgcgc gcgccggctc gaccagcagg gtcccgcccc
      781 atccgaaggt gaccccgcgg gcttcctggc gctcgacgcg gaagatgtcc tcgatgttgc
      841 gccgcagcag gtcctcgatg atcgggaagt gctcgagcgg cgcctgctcg gacgattgaa
      901 acggccagcg gcgcatgagt gcaggttacc acacggccag gagggcgatc gcgcgccggc
      961 ctgcgtcgag cccgccctcg agatagacca ccccgagcac cgcttcgagc gcggtggcga
     1021 gcaccgactc cttggcgcgc ccgcccatct gctcctcgcc gcggccgagc cgcaggtgca
     1081 cgcccagatc cagcgcggcc gcccagcgcg cgaggttctc gcccgagacc agggcggccc
     1141 gccgcggggt gagcacgccc accgggtccg ccggcgcgat cgcgagcagg cgctcggcca
     1201 ccaccaggga cagcatcgcg tccccgagca gggcgagccg ctcgttgtcg cggctgcccg
     1261 cgatctcgtt ggcgaaggag cggtgggtga gcgcctcccg caggagatcc gggtcccgga
     1321 aggtatggcc gagccggcca cccagcgcgt cccgcgggtc ggccgcggcc gtcaactagg
     1381 ggcgatactt ccggaggacg atggtggcgt tggtgccgcc gaagccgaac gcgttgctga
     1441 gcgcgacctc cacgtcctgc ttgcgcgcct ggttcggcac gtagtcgagg tcgcagtccg
     1501 ggtcgggcgt ctcgtagttg atggtgggcg gcaggatccc gtggtggatg gcgagcgcgg
     1561 tggcgatcgc ctcgatcccg cccgcggcgc cgagcaggtg cccggtcatc gacttggtgg
     1621 acgacaccgc gagcttgcgc gcgtgctcgc cgaacacccg cttgatggcc atggtctccg
     1681 acttgtcgtt gtagggcgtc gacgtcccgt gggcgttgat gtagcccacc gcggacggct
     1741 cgacctccgc gtccttcatg gcgagcgcca tcgcgcgggc cgccccgtcc gcctccgggt
     1801 ccggcgcggt catgtggtgg gcgtcgccgg tcatgccgta gcccaccacc tccgcgtaga
     1861 tgcgcgcgtc acgaccgagg gcgtggtcga gcgactccag caccaggagc ccgcccccct
     1921 ccccgcacac gaagccgtcc cgggtggcgt cgaacggccg actggcgcgg cccggatcat
     1981 cgttcctggt ggacatcgcc ttcatctggc agaagcccgc gaaggtgagc ggcaggatga
     2041 tcgcctccga cccgcccgcg atcatgatgt cggcgtcgtt gcgctggatg atcttcatcg
     2101 cgtcgccgat cgcgtggttg ccggtcgcgc acgcggtgac gatcgacgag ttcggcccct
     2161 tggccccgaa gcgcatcgag atcagccccg aggccatgtt cacgatcagc atcgggatga
     2221 agaacgggga gacgcgatcc gggcccttct cgagcagggt cttgtgggtc tcgagcagcg
     2281 tggtgatgcc accgatgccg gagcccacca gcaccccgaa gcgggtgcgg tccaccttct
     2341 ccacgttcag gcccgcatcc tccaccgcca tcgccgcgca ggcgatcgcg tacttgagga
     2401 agggatcgag cttgcgatcg tccttcttgt ccatgtagcg gagcgggtcg aaccccttca
     2461 gctcgcccgc gatcttggtg ggcagcggag aggcgtcgaa ccgggtgatg gggccgatcc
     2521 ccgacttccc ctggatcagc gcggcccaga actcctcggc cgtgttgccc acgggagtga
     2581 ccgcccccac cccggtgatg acgacgcgac gcagctccac cgtcttcatc ccgccccgcc
     2641 cgcctactgg ttctctttga tgtagctgat ggcgtccccg acggtggcga tcttctccgc
     2701 gtcctcgtcg gggatctcca gatcgaactc ctcctcgaac gccatcacga gctcgacggt
     2761 gtcgagcgag tccgccccca ggtcctcgat gaacttggcc gcgggcagca cctcgtcctc
     2821 ctccactccg agctgctcga cgatgatctc cttcacgcgc tcttcaaccg gcttcgccat
     2881 gccctccctc ctgactgcga gtgattctgg attcgctcag acgtacagcc cgccgttgac
     2941 gtggatcgtc tggcccgtga tgtagctcgc cccgtctccg gccaggaacc gcaccacctc
     3001 cgccacctcg cgcccctggc cgatccgctt cagcgggacc tgctggagca tcgcctcccg
     3061 cgcctccgcg ggaatcgacg ccgccatgtc ggtctcgatg aggccgggcg ccaccgcgtt
     3121 caccaggatg ttccagtgcg ccagctcgcg ccccgccgcc ttggtcagcc cgatcacgcc
     3181 cgccttggcc gccgagtagt tcacctgtcc cggattgccc atcgcgcccg cggtggacgc
     3241 gatgttgatg atgcgcccgc tcttctgcct catcatcacc ttgccgaccg cgcgggtcat
     3301 caggaacacg ccccgcaggt tcgtgtcgag cacctggtcc cagtcgtcgt ccttcatgcg
     3361 gaccaggagc tgatcccggg tgatccccgc gttgttcacc agcacgtcga tgcgcccgaa
     3421 gccctcccgg gtcgcctcca cgaggcggtc ggcgtcctcg cgcttcgccg cgtccgccgc
     3481 caccgccatc gcgggcgcgc ccagcgattc cagctctttg accgccgcct ccagacgcgc
     3541 cgcgtcgcgg ccagagacta ccacggaggc gccgtcctct gccagcaacc gggcgatcgc
     3601 gagaccgatt ccgcgagtgc cgccggtgac gatggcgacc ttgccggcca gggtcttccc
     3661 ctcgctcacg gctcccctcc cgccagtgcg gccaccgcct tctcgagcga cgcgggatcc
     3721 tcgaccgcgt ggccgcgcac cccgtcgagg atgcgcttga gcagaccggt cagcacccgc
     3781 ccggggccca cctcgaggaa cgcggtggcc ccctcgcgcg agagacgctg cgtgcagtcg
     3841 gtccagcgca ccgggctcgc cacctgctgg accaggaagg gcttcacctc gtctgcagag
     3901 atggtgaccc cgccgtccac gttgcgcacg atccggatgc ggggcgccga gaccgcgacc
     3961 cggtcgagct cggcggcgag gcgatccgcc gccggcttca tgagcgcgca gtggaagggc
     4021 gcgctcaccg ggagcatcac gctcttcttg cccccgcgcg cgcccgcgcc cgccaccgcg
     4081 cgctccaccg cggtgcgatg gccggccacc acgatctggc ccggcgagtt gatgttggcc
     4141 acctccacca cctcgccctg cgcggcctcc gcacacacct gctcgacggt gggcaggtcc
     4201 acccccatca gggcggccat cgccccggtg cccaccggca ccgcctcctg catgaactcg
     4261 ccgcgccgcc gcaccaggcg caccgcgtcc ttgaaggaga gcgccccgcc caccaccagg
     4321 gccgagtact cgcccaggct gtgaccggcg gcgaggtcgg gggtcacgcc ccgctcggcg
     4381 agcgcggccg ccgcggccac gctggcggtc agcaccgcgg gctgggtgtt ggcggtcagg
     4441 gccagctcct cggccggccc ctcgaagcag aggcgcgaga gcggaaagcc gagcgcggca
     4501 tcggcctcgg cccacaccgc gcgcgcgccg ggcgaggcct ccgccagggc gcggcccatc
     4561 ccgacctcct gcgagccctg gccggggaag aggaaggcga tcgggcccgc taccatcgca
     4621 gcagcgccgc cccccacgtg aacccgcccc cgaaggcggc gagcaggatg agatcgcccg
     4681 gcttgacccg gccgttcgtc cacgcctcct cgagggccac gtagaccgag gccgcgccgg
     4741 tgttgccgta gcgctccacg ttcaccatca cgcgctccat cggcaccttc aggcgcttca
     4801 ctgccgcctc gatgatgcgg aggttcgcct ggtggggcac gaagaggtcg aggtcggcgg
     4861 cggtgaagcc gttgttcttc aggatgcgct cggcggactc gccgaacatg cgcaccgcga
     4921 ccttgaagac ctcgttgccc ttcatcctgg cgaagtgcat ccgcttgtcg accgtttcgt
     4981 gggtcgccgg gtggcgcgag ccgcccgccg gttggatcag cagctcgccg tagcgcccgt
     5041 cggagtagag gtcggtatcg aggatcccgg cgtctccgtc ggaggggcgg agcaccaccg
     5101 cgccggccgc gtcggcaagg aggatgcagg tgccgcgatc ggtgaagtcg gtgatgcgcg
     5161 agaggcactc ggccccgatg cagagcacgg tgcggta
//
LOCUS       DATMCV010000352         4679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_551825, whole genome shotgun sequence.
ACCESSION   DATMCV010000352 DATMCV010000000
VERSION     DATMCV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551825"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..287
                     /locus_tag="VNU03_08205"
     CDS             99..287
                     /locus_tag="VNU03_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74483.1"
                     /translation="MKPKIPWLPHEVQPGQPTEVCPRCGARKLMPWTLRRDPKRVTLL
                     RTWVCTACQTTEEREEPE"
     gene            complement(309..1343)
                     /locus_tag="VNU03_08210"
     CDS             complement(309..1343)
                     /locus_tag="VNU03_08210"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HWP74484.1"
                     /translation="MSADRRYQLQSEDANRRLDALLEHLRVPEASWPLYAEMLTTALK
                     MFEDGTGVADLKIANAAFKEIRYGFKVFAPWRNVPKVTVFGSARTPREHPISEQAYAF
                     GKRITGAGWMVMTGAGGGVMSSAQEGAGGERSFGLNIRLPFEQEANPWIASDPKLISF
                     KYFFTRKLFLVREAHAMAFLPGGFGTADEAFEALTLIQTGKSPVLPLVMLDEPGGTYW
                     KTFDTFVRREMVGRGMVGSSDPDLYRITDDAETAAAEVEAFYRNYHSQRYVRDSLVLR
                     MRRPLAAETLAELNARFTDILSGPATQAAGPVPGEGDELPDLPRLVLPFNRSGFSRLR
                     SLIDVVNAPA"
     gene            complement(1369..2025)
                     /gene="phoU"
                     /locus_tag="VNU03_08215"
     CDS             complement(1369..2025)
                     /gene="phoU"
                     /locus_tag="VNU03_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWP74485.1"
                     /translation="MSRHFHEELEALKQTLLAMGGLVEDQIRRVMRALLERDDVMAQE
                     VIERDRQVNTYDVEVDEQCVSLLALHQPAAGDLRFITTAMKIVTDLERIGDQAVNIAQ
                     RVLELNREPQLKPYIDLPRMAEKAQRMVKESLDAFVARDTALARQVCGEDAEVDALKE
                     QIFRELLTFMMEDPRTIPRAIRVILISRFMERVADHATNIAEMVVYLVEGKMVRHTLA
                     "
     gene            complement(2038..2796)
                     /gene="pstB"
                     /locus_tag="VNU03_08220"
     CDS             complement(2038..2796)
                     /gene="pstB"
                     /locus_tag="VNU03_08220"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWP74486.1"
                     /translation="MGDGIYVKGLRAWFGETLVLKGVDLTVAPNAVTAIIGPSGCGKS
                     TFIRCVNRMHETVPGARVAGQVLLNSEDLYAPGVDPVQVRRRIGMVFQKANPFPTMSV
                     FDNVAAGLRLAGVRRKSRLQEEVERALRHAALWDEVKDDLDKSGVSLSGGQQQRLCIA
                     RALAVDPQILLMDEPCSALDPIATAKIEELIHDLKKELTIAIVTHNMQQAARVSEWTG
                     FFLLGELVEFGITKEMFTTPGDKRTEDYITGRFG"
     gene            complement(2804..3607)
                     /gene="pstA"
                     /locus_tag="VNU03_08225"
     CDS             complement(2804..3607)
                     /gene="pstA"
                     /locus_tag="VNU03_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWP74487.1"
                     /translation="MSGLTAGAALLVVCPLLLIFGFLLYQGVSAVNVDFFLHLPKPVG
                     EPGGGVANAIVGSLIMIGLAVSIGLPVGILGGVYLAESRDRRLPWVLRFVADVMNGVP
                     SIVFGIFAYTVVVLPMRRFSALAGGFALGLILIPIVLRTSEEMVRLVPSALREAGLGL
                     GLPEWKVSLRIVIPAARAGIITGVMVAVARIAGETAPLLFTALGNRFWHQGLDQPIAA
                     LPLQIYAYAIAPYDDWHRQAWAGALVLITLVFLASLAARLATRGRIVAR"
     gene            complement(3640..4635)
                     /gene="pstC"
                     /locus_tag="VNU03_08230"
     CDS             complement(3640..4635)
                     /gene="pstC"
                     /locus_tag="VNU03_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWP74488.1"
                     /translation="MAADSISGVGTADRLSLPPRTVSRGRSAFGDRVYRTLILASVWL
                     VLILAAGLVAALVWESWTAMRAFGLRFLVTSHWDPVAGEFGALPFIYGTLVSSLLALL
                     IAVPLSLGAAVFLAELAPAWIRPPVAFLIEMLAAVPSVVYGLWGIFVLVPWLRDWVQP
                     ALGRSLGFLPLFQGPPYGIGMLAAGLILSIMIVPYITSVSREVLLAVPGSQREAALGL
                     GATRWETTRIAVLRYGRSGLIGAILLGLGRALGETMAVTMVIGNRPEVSVSLFAPGYT
                     MASVLANEFTEATSDLYVSALVEIGLLLLVVTVLVNGLARLLVWSVGGPARGARG"
BASE COUNT          762 a         1684 c         1599 g          634 t
ORIGIN      
        1 ctcgggttct acgcccggct ggtctgggcg atggtccagc gggtccgcca gcggtaggcg
       61 cgcgctcgcg ggcacgctct tggcgtataa tcgcgcgcat gaagcccaag atcccgtggc
      121 tcccgcacga ggtgcagccg ggtcagccga ccgaggtgtg cccgcgctgc ggcgcccgca
      181 agctgatgcc gtggacgctg aggcgcgatc cgaagcgcgt caccctgctc cgcacgtggg
      241 tctgcaccgc ctgccagacc accgaggagc gcgaggagcc ggagtaggtc ggagacggcg
      301 gagacgcgtc aggccggcgc gttcaccacg tcgatcaggc tccgcagccg cgagaacccg
      361 gagcggttga agggcagcac cagcctcggc aggtccggca gctcgtcccc ctcgcccggc
      421 accggaccgg ccgcctgcgt cgcgggcccg ctcaggatgt cggtgaagcg cgcgttcagc
      481 tcggcgaggg tctcggccgc cagcgggcgg cgcatccgca gcacgagcga gtcgcgcacg
      541 tagcgctgcg agtggtagtt ccggtagaac gcctcgacct ccgccgccgc cgtctccgcg
      601 tcgtcggtga tccggtagag gtcgggatcg ctcgacccga ccatgccgcg gcccaccatc
      661 tcgcgccgca cgaaggtgtc gaacgtcttc cagtaagtgc cgcccggctc gtcgagcatc
      721 accagcggca gcaccggcga cttgccggtc tggatcaggg tcagcgcctc gaaggcctcg
      781 tcggcggtcc cgaagccgcc gggcaggaac gccatcgcgt gcgcctcccg caccaggaag
      841 agcttgcggg tgaagaagta cttgaacgag atcagcttgg gatcggaggc gatccacgga
      901 ttcgcctcct gctcgaacgg caggcggatg ttgagaccga acgagcgctc gccgcccgcg
      961 ccctcctggg ccgagctcat gaccccgccg cccgcgccgg tcatgaccat ccaccccgcc
     1021 ccggtgatgc gcttgccgaa ggcgtacgcc tgctcggaga tcgggtgctc gcgcggggtg
     1081 cgggccgagc cgaacacggt caccttcggc acgttccgcc acggggcgaa gaccttgaag
     1141 ccgtagcgga tctccttgaa cgcggcgttc gcgatcttga ggtcggccac tccggtcccg
     1201 tcctcgaaca tcttgagcgc ggtggtgagc atctccgcgt agagcggcca cgaggcctcc
     1261 ggcacgcgca ggtgctcgag gagggcgtcg aggcgccggt tggcgtcctc ggactgcagc
     1321 tgatagcgac gatccgcact cacacttgca gtatacacgc gcgtcctctc aggccagtgt
     1381 atgccggacc atcttgccct cgaccaggta cacgaccatc tccgcgatgt tggtggcgtg
     1441 gtcggccacc cgctccatga agcgggagat caggatcacc cggatcgcgc gcgggatcgt
     1501 ccgggggtcc tccatcatga acgtgagcag ctcccggaag atctgctcct tgagcgcgtc
     1561 gacctcggcg tcctcgccgc acacctgccg ggccagcgcg gtgtcgcggg ccacgaacgc
     1621 gtcgaggctc tccttcacca tccgctgcgc cttctcggcc atgcggggca ggtcgatgta
     1681 gggcttgagc tggggctccc ggttcagctc gagcacccgc tgggcgatgt tcaccgcctg
     1741 gtcgccgatg cgctccagat cggtcacgat cttcatcgcg gtggtgatga agcgcaggtc
     1801 gcccgcggcg ggctggtgga gcgcgagcag gctcacgcac tgctcgtcga cctccacgtc
     1861 gtaggtgttg acctgccggt cccgctcgat gacctcctgg gccatcacgt cgtcgcgctc
     1921 gagcagggcc cgcatgaccc gccggatctg gtcctccacg agcccgccca tcgcgagcag
     1981 ggtctgcttg agggcctcga gctcttcgtg gaaatgccgt gacatggcgt cctccgctta
     2041 cccgaaccga ccggtgatgt agtcttccgt ccgcttgtcg cccggggtcg tgaacatctc
     2101 cttggtgatc ccgaactcca cgagctcgcc gaggaggaag aagcccgtcc actccgagac
     2161 gcgcgcggcc tgctgcatgt tgtgggtgac gatcgcgatc gtcagctcct tcttgagatc
     2221 gtggatcagc tcctcgatct tggcggtggc gatggggtcg agggccgagc acggctcgtc
     2281 catcagcaag atctgcggat ccaccgcgag ggcgcgggcg atgcagagcc gctgctgctg
     2341 gccgcccgag aggctcaccc cggacttgtc cagatcgtcc ttcacctcgt cccacagggc
     2401 cgcgtggcgg agcgcccgct ccacctcctc ctgcagccgc gacttccggc gcacccccgc
     2461 gagccggagg ccggccgcca cgttgtcgaa gacggacatg gtggggaacg ggttggcctt
     2521 ctggaagacc atgccgatcc gccggcgcac ctgcaccgga tccacccccg gcgcgtagag
     2581 gtcctcgctg ttgagcagga cctggccggc cacccgcgct cccggcaccg tctcgtgcat
     2641 ccggttgaca cagcggatga acgtggactt gccgcagccc gacggcccga tgatggcggt
     2701 caccgcgttc ggcgcgaccg tgaggtcgac gcccttcagg accagggtct cgccgaacca
     2761 ggcccgcagc cccttgacgt agatgccgtc tcccatggtc acctcagcgg gcaacgatcc
     2821 ggcctcgcgt ggccagcctc gccgccagac tcgccaggaa caccagcgtg atcagcacga
     2881 gcgccccggc ccaggcctgc cggtgccagt cgtcgtaggg tgcgatggcg tacgcgtaga
     2941 tctgcagcgg cagcgccgcg atgggctggt cgagcccctg gtgccagaac cgattgccga
     3001 gcgcggtgaa cagcaggggc gcggtctccc ccgcgatccg ggccaccgcc accatgaccc
     3061 cggtgatgat gcccgcgcgg gccgccggga tgacgatgcg caggctcacc ttccactccg
     3121 gcaggccgag cccgaggccg gcctcccgca gcgcgctcgg caccaggcgc accatctcct
     3181 cgctggtgcg gagcacgatc gggatcagga tcaggccgag cgcgaagccg cccgcgaggg
     3241 ccgagaagcg ccgcatcggg agcacgacca cggtgtacgc gaagatgccg aagacgatcg
     3301 agggcacccc gttcatcacg tcggccacga accggagcac ccacggcagc cggcgatcgc
     3361 gggactcggc caggtacacc ccgcccagga tccccaccgg cagcccgatc gacaccgcga
     3421 gaccgatcat gatcaggctg cccacgatcg cgttggccac gcccccgccg ggctcgccca
     3481 ccggtttcgg caggtgcagg aagaagtcca cgttgaccgc cgacacgccc tggtagagga
     3541 ggaagccgaa gatgagcagc agcgggcaga ccacgagcag cgccgcgccg gcggtgaggc
     3601 cgctcaccgc agtgctgacg agccggcgcc cgccggggct catccccgag caccgcgggc
     3661 cgggccgccc acgctccaca ccaggagacg ggccagcccg ttgacgagca cggtgaccac
     3721 gagcagcagc aggccgatct cgaccagggc tgacacgtag aggtccgacg tggcctcggt
     3781 gaactcgttc gcgagcacgc tcgccatggt gtagccgggc gcgaacagcg agaccgagac
     3841 ctcggggcga ttgccgatca ccatggtcac cgccatggtc tcgccgaggg cccggccgag
     3901 cccgaggagg atcgcgccga tgagcccgga gcgaccgtag cgcagcaccg cgatccgggt
     3961 cgtctcccac cgcgtggcgc cgagcccgag cgcggcctcg cgctgggagc ccggcacggc
     4021 gagcagcacc tcgcggctca ccgaggtgat gtagggcacg atcatgatcg acaggatcag
     4081 ccccgccgcg agcatcccga tgccgtaggg cggcccctgg aagagcggca ggaagccgag
     4141 gctccgtccg agcgcgggct gcacccagtc ccgcagccac gggaccagga cgaagatccc
     4201 ccagaggccg tagaccacgc tcggcaccgc ggccagcatc tcgatcagga aggccaccgg
     4261 cggccggatc caggccggcg ccaactccgc gaggaagacg gccgcgccga gcgacagcgg
     4321 gaccgcgatc agcagcgcca gcagcgacga caccagggtg ccgtagatga acgggagcgc
     4381 gccgaactcg cccgcgaccg gatcccagtg cgaggtgacc aggaagcgga gcccgaaggc
     4441 gcgcatggcg gtccacgact cccacacgag cgccgcgacg agccccgcgg ccagaatcag
     4501 gaccagccaa accgaggcca ggatcagggt gcggtaaacc cggtcgccga atgcgctgcg
     4561 cccccgactg accgttcgcg ggggcaggga cagcctgtcg gccgtcccca cccccgagat
     4621 cgagtcggcg gccaagcgct actgcgccgc caggagcggc ttgccggaga aggtgatct
//
LOCUS       DATMCV010000353         3434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_485399, whole genome shotgun sequence.
ACCESSION   DATMCV010000353 DATMCV010000000
VERSION     DATMCV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485399"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VNU03_08235"
     CDS             <1..537
                     /locus_tag="VNU03_08235"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWP74489.1"
                     /translation="PDSPMAMDNDSRPRVWRKVGEEILAHPLTGAGFGRGVLSKAYPA
                     LLPADESQVFWHPHNLVLTYGVSAGVPGMVAVLALFASLAWRFWQLACRGEPLARLAG
                     LAGTVMVAGVLARNMTNDFFVRDGALLFWALAGMLFGYALRRPPASGADEADLRPARQ
                     GVVVGGAATGDRGRERAA"
     gene            534..1199
                     /locus_tag="VNU03_08240"
     CDS             534..1199
                     /locus_tag="VNU03_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HWP74490.1"
                     /translation="MSHHAPRFRECDDVLAEANDGVVVIGAGGHAKVLISTLAACGVP
                     VAAVVDDDDTKWGMDAQGTRVGRIERELGGRGIVGIGDNAQRREMAGALSLDWQTVVH
                     PSAYVHPSAKLGRGTVVFAGAVVQPDAVIGDHVIVNTGATIDHDCIVGDYAHLAPGVH
                     LAGAVQVGEGAFLGIGSVVCPGIKVGRWATLGAGGVAIRDVADGVVAVGVPARALPGE
                     RPS"
     gene            1196..2080
                     /locus_tag="VNU03_08245"
     CDS             1196..2080
                     /locus_tag="VNU03_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015196820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWP74491.1"
                     /translation="MSPPILTAEQLALLPDDSDVASYEMNGYYVSKEGVVPEALIDAA
                     WDGAHRFYQGERDGTLAVDTGFSNWKPGDGDGQRNNEFVSLQKKELKALAVYPLLGAI
                     AAKLTRSSAIRVLDDQLIYKPSDTRDASSTVTGWHADRAYWATCSSDKMISVWIPLHS
                     VEITRSPLVVMAGSHRWQGLEDVRFFNHRDLAEIEERFRAGGRDVRIVPMTLRKGQVS
                     FHHGWTVHASYPNTSGLPRVSYAVHLQDGDNRYRPYRNREGREIHIFDETLCRRLANG
                     DPDFSDPAVFPTVWAERP"
     gene            2080..3132
                     /locus_tag="VNU03_08250"
     CDS             2080..3132
                     /locus_tag="VNU03_08250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP74492.1"
                     /translation="MDVEHLAIDDPRWPDALRRLRHDFYHLPEYVRLDGEWNRAQPMA
                     FLARSGDEELFIPYLLRRCESFGPESMAGEEVYDVVSPYGYPGLLLSDAARRSPRFAR
                     EAMRRLSETLREMGACSAFLRMHPLLSDGLSDLFPEGFFTPAGETVAMDLTLDDAGLW
                     KGVREGHQWTIKKCRRLGFVARMVSLRAYVECVMEIYRETMDRVQAKESYYFGRDYFA
                     RLAEMPGHVHCGVVESEGRPAAACIFFECGGIVQAHLGGTRSEFLNRSPFHLLLYSAA
                     EWARSRGNRYLHIGGGVGGSNDRLLQFKRGFSPLRFPFSTLRLVTDEGKYRELTTRRA
                     QAAAVSDFFPAYRAPV"
     gene            complement(3154..>3434)
                     /locus_tag="VNU03_08255"
     CDS             complement(3154..>3434)
                     /locus_tag="VNU03_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74493.1"
                     /translation="VVSGAGAQYMFSAAPGRLVTTIVNNSGVEWNGAITASIPGAVTA
                     VREYAADTAAACARTGPTVTVKGSVPAYDLRIFAIEYAPGAPAPGPAC"
BASE COUNT          504 a         1130 c         1253 g          547 t
ORIGIN      
        1 ccggattcgc cgatggcgat ggacaatgat tcgcggcccc gggtgtggag gaaggtgggc
       61 gaggagatcc tcgcgcaccc gttgaccggc gccggattcg gacgcggcgt cctgagcaag
      121 gcctacccgg ccttgcttcc ggccgacgag agccaggtgt tctggcaccc tcacaatctc
      181 gtgctcacct acggcgtcag cgccggcgtg ccgggcatgg tggcggtgct ggcattgttc
      241 gcctcgctcg cctggcgctt ctggcagctg gcgtgtcgcg gcgagcctct cgcgcgtctg
      301 gccgggcttg cggggacggt catggtcgcg ggcgtgctgg cccgcaacat gaccaatgac
      361 ttcttcgtgc gggatggggc gttgctgttc tgggcactcg ccggaatgct cttcggatac
      421 gcgctgcgtc gtccgcccgc gtcgggagcg gatgaagccg atcttcggcc cgcgcggcaa
      481 ggcgtcgtgg tcgggggcgc cgccactggc gatcgcggcc gcgagcgggc cgcatgagtc
      541 accacgcccc gcgcttccgg gagtgcgacg acgtgctcgc ggaggcgaac gacggcgtcg
      601 tggtcatcgg cgcgggaggc cacgccaagg tgctcatcag caccctcgcc gcctgcggag
      661 tgccggtcgc ggcggtggtc gacgacgacg acaccaagtg gggcatggac gctcaaggta
      721 cccgcgtggg ccggatcgag cgcgagctcg gcggccgcgg gatcgtgggg atcggggaca
      781 acgcgcagcg ccgggagatg gcgggggcgc tcagcctgga ctggcagacg gtggtccatc
      841 cgtccgccta cgtgcacccg tcggcgaagc tcgggcgggg cacggtggtc tttgccggcg
      901 cggtcgtgca gccggatgcg gtgatcggcg accacgtcat cgtgaacacg ggcgccacga
      961 tcgaccacga ctgcatcgtc ggcgactacg cgcatctcgc gcccggcgtg cacctcgcgg
     1021 gcgcggtgca ggtcggagag ggcgcgtttc tcggcatcgg aagcgtggtg tgcccgggca
     1081 tcaaggtcgg gcgctgggcc accctcggag cgggaggggt ggcgattcgc gacgtggcgg
     1141 acggagtcgt ggcggtcggc gttccggccc gagcgctccc gggcgagcgt ccgtcatgag
     1201 cccgccgatc ttgaccgccg agcagctcgc gctcttgccc gacgactcgg acgtggcctc
     1261 ctacgagatg aacgggtact acgtctcgaa ggagggggtc gtgccggagg ccctgatcga
     1321 cgcggcctgg gacggggccc accggttcta tcagggggag cgggacggca ccctggccgt
     1381 cgacaccggc ttctcgaact ggaagccggg cgacggagac ggccagcgaa acaacgagtt
     1441 cgtgtcgctg cagaagaagg agctgaaggc tctcgcggtc tacccgctcc tgggggccat
     1501 cgcggcgaag ctgacccgga gctccgccat tcgcgtgctg gacgatcagc tcatctacaa
     1561 gccgtccgac acccgggacg cctcgagcac ggtgaccggc tggcacgcgg accgggccta
     1621 ctgggcgacc tgcagctccg acaagatgat ctcggtgtgg attccactcc actcggtgga
     1681 gatcacccgc agcccgctcg tggtgatggc ggggagccat cggtggcagg gtctggagga
     1741 cgtgcggttc ttcaaccacc gggatctggc cgagatcgag gagcggttcc gcgcgggcgg
     1801 ccgggacgtt cgcatcgtcc ccatgaccct gcgcaagggg caggtgagct tccatcacgg
     1861 ctggacggtc cacgcgagct atcccaacac gagcggactc ccgcgggtgt cgtatgcggt
     1921 ccacttgcag gacggcgaca atcgctatcg tccctaccgc aaccgcgaag ggcgggagat
     1981 ccacatcttc gacgagacgc tgtgccgccg gctggccaac ggcgatccgg acttcagcga
     2041 cccggcggtg tttcccaccg tctgggcgga gaggccctga tggacgtcga gcacctggcg
     2101 atcgacgatc cgcgatggcc cgacgccctg cgccggctgc gccacgattt ctaccatctc
     2161 cccgagtacg tgagactgga cggcgagtgg aaccgggcac agccgatggc gttcctggcc
     2221 agatccggag atgaggagct gttcattccc tatctgctgc gccggtgcga gagcttcggg
     2281 ccggaatcca tggcgggcga agaggtgtac gacgtcgtgt cgccctatgg gtacccgggc
     2341 ctgctgctga gcgacgcggc ccgacggtcc ccccggttcg cccgggaagc gatgcgtcga
     2401 ttgagcgaga ccctccgcga gatgggcgcg tgctccgcgt tcctccgcat gcatcccctg
     2461 ctgagcgacg ggttgtcgga cctcttcccg gagggattct tcaccccggc cggcgagacg
     2521 gtcgcgatgg atctgaccct ggacgacgcc ggattgtgga agggggtccg ggaagggcac
     2581 cagtggacga tcaagaagtg caggcgcctg ggcttcgtgg cccgcatggt ctcactgcgc
     2641 gcgtacgtcg agtgcgtcat ggagatctac cgggagacga tggaccgggt ccaggccaag
     2701 gagtcctact acttcggccg ggactacttc gcgaggctcg ccgagatgcc cgggcacgtg
     2761 cattgcgggg tggtggaatc ggagggccgg cccgcggccg cctgcatctt cttcgagtgc
     2821 ggcggaatcg tgcaggccca cctgggcggc acccggtcgg aatttctcaa caggtcaccg
     2881 tttcacctgc tcctgtattc cgccgccgag tgggccaggt cccgcggcaa ccggtacctc
     2941 cacatcgggg gaggcgtcgg cggctccaac gaccgcctcc tccagttcaa gcgcggattc
     3001 tcgccgttgc gctttccgtt ctcgacgctg cgtctcgtca ccgatgaagg gaagtaccgt
     3061 gagctgacga cacgccgggc gcaggccgcc gcggtgtcgg acttctttcc cgcctaccgg
     3121 gctcccgtct gagaggcacc gccgctccgg ggctcagcac gccgggccgg gcgcgggcgc
     3181 gccgggcgcg tattcgatcg cgaagatccg gagatcgtag gcgggaacgc tccccttgac
     3241 cgtcacggtg ggcccggtgc gcgcacaggc cgccgcggtg tcggcggcat actcgcgaac
     3301 cgcggtgacg gcgccgggga tgctcgcggt gatcgcgccg ttccactcca cccccgagtt
     3361 gttgacgatc gtggtgacga gccgccccgg cgcggccgag aacatgtact gagccccggc
     3421 accggacacc acgg
//
LOCUS       DATMCV010000354         2972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_287088, whole genome shotgun sequence.
ACCESSION   DATMCV010000354 DATMCV010000000
VERSION     DATMCV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287088"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VNU03_08260"
     CDS             <1..380
                     /locus_tag="VNU03_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP74494.1"
                     /translation="RAMATRLRMPPEISGGMRKRVAIARALVTEPEIVFFDEPTTGLD
                     PILVNTIHHLIVDLHRKFRFTAVMVSHEIPEIFEIADRVAMLHDGTIVEVGPPEAVRG
                     STKSVVQQFIRGDIEAVPAEHHR"
     gene            396..836
                     /gene="mlaD"
                     /locus_tag="VNU03_08265"
     CDS             396..836
                     /gene="mlaD"
                     /locus_tag="VNU03_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HWP74495.1"
                     /translation="MKRSALDLGVGVFVLIGLIALGWMSVKLGRVDLLGNRGYVVSAD
                     FPTVGGLKAGSTIEIAGVEVGRVDRIVLQDYQAHVVMVIQPGVKLQDDSIASIKTKGL
                     IGERYVRISPGGSDKIIPPNGRIREVEPPVDIEELLSKYIFGKV"
     gene            859..1494
                     /locus_tag="VNU03_08270"
     CDS             859..1494
                     /locus_tag="VNU03_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74496.1"
                     /translation="MNWFHVKTALLALGLAVITAVPATAGVPTDQLRGAVDRVLKTLD
                     DPSLKGADKVADRRVAVRKIANEIFDFGEIAKRSMARHWQPLSEPQRTEFVGLFADLL
                     ERSYISKIETYGGEKIQYTGERIDGEYATVSTRIITKNGTEVPVDYRMKKGGDRWLVY
                     DVSIEGVSLVSSYRTQFNKIIQTSSYNELVSKLKNKQDELLAEDKGAKKKL"
     gene            1491..>2972
                     /locus_tag="VNU03_08275"
     CDS             1491..>2972
                     /locus_tag="VNU03_08275"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWP74497.1"
                     /translation="MTKSGLLRAILRRPGLVPMVGAHDALSAKLIEAAGFPVVWSSSF
                     AVSAAQRAVPDVNLLTMTENLEAALFIDRAVAAPVIADVDNGYGNVVNVVRTVREYER
                     AGIAGICMEDNVFPKKCSLYPGMKRELLSVEEFAGKVRASVDTRQDPDFVVIARVEAL
                     IAGWGMKEALRRAHAYADAGADMVLMHSKSKTAAEILEFMRLWDRPTPVVVVPTLYPS
                     VTFEELEQAGVKMVIWANHVLRGAVQGMQQTLATLARERRLDALDPHIVPLEEIYKHV
                     GVDEFKAIEDAYLPASAGPARAIIIAAGSGKSLLPLTEDRPKCMLDIKGRTILERQID
                     TLRACGVQDIVVVRGYKKDMVTVPGVRYYDHDRYEETGELGSLFAAERELRGPVLFLY
                     SDILFEQTVLDKLLRAETDCAIVVDRAWVDQRERLLPLGKPLDLVVTARTPEAGLRAL
                     PGAPDDRLVSVGQRIAPERATGEFIGLALFSERGTTAMREAYQQ"
BASE COUNT          498 a         1019 c         1018 g          437 t
ORIGIN      
        1 cccgcgcgat ggcgacccgc ttgcgcatgc cccccgagat ctcggggggc atgcgcaagc
       61 gggtcgccat cgcgcgggcc ctggtgaccg agccggagat cgtcttcttc gacgagccga
      121 ccaccggact ggatccgatc ctggtcaaca cgatccacca tctgatcgtg gacctgcacc
      181 gcaagttccg gttcaccgcg gtcatggtga gccacgagat cccggagatc ttcgagatcg
      241 cggaccgggt cgcgatgctc cacgacggca cgatcgtcga ggtcggcccg cccgaggcgg
      301 tgcggggctc gacgaaatcg gtcgtccagc agttcatccg gggcgacatc gaggcggtgc
      361 ccgcggagca ccatcggtag tcggaggaga tcacgatgaa gcgatcggca ctggatctgg
      421 gcgtgggcgt gttcgtgctc atcggcctga tcgccctggg atggatgtcg gtgaagctcg
      481 gccgcgtcga tctcctgggc aaccggggct acgtggtgag cgcggacttc cccaccgtgg
      541 gcgggctgaa ggcgggctcg accatcgaga tcgcgggggt cgaggtgggc cgggtggatc
      601 gcatcgtgct gcaggactac caggcccacg tggtcatggt gatccagccc ggggtgaagc
      661 tgcaggacga ctcgatcgcc tccatcaaga ccaagggcct gatcggcgag cgctacgtcc
      721 gcatcagccc gggcggctcg gacaagatca tcccgcccaa cgggcggatc cgggaggtcg
      781 agccgccggt cgacatcgag gagctgctct ccaagtacat cttcggcaag gtgtagactc
      841 tcgaaccggg aggacgcgat gaattggttt cacgtgaaga ccgcactgct cgccctgggg
      901 ctcgccgtga tcaccgcggt ccccgccacg gcgggagtgc cgaccgacca gctccggggc
      961 gcagtggacc gcgtcctcaa gaccctggac gacccgagtc tcaagggggc ggacaaggtc
     1021 gccgatcggc gcgtcgccgt ccggaagatc gccaacgaga tcttcgactt cggcgagatc
     1081 gcgaagcgct cgatggcccg ccactggcag ccgctctccg agccccagcg caccgagttc
     1141 gtcggcctct tcgcggacct gctcgagcgc tcctacatct cgaagatcga gacgtacggg
     1201 ggcgagaaga tccagtacac cggggagcgg atcgacggcg agtacgccac cgtgtccacc
     1261 cggatcatca ccaagaacgg caccgaggta ccggtcgact accgcatgaa aaagggggga
     1321 gaccgctggc tcgtctacga cgtgagcatc gagggcgtca gcctggtctc cagctaccgc
     1381 acccagttca acaagatcat ccagacctcc tcctacaacg agctcgtctc caagctgaag
     1441 aacaagcagg acgagctcct cgcggaggac aagggggcga agaagaagct atgaccaaga
     1501 gcggcctcct ccgcgcgatc ctgcgtcggc cgggtctggt gcccatggtg ggcgcccacg
     1561 acgcgctctc cgccaagctc atcgaggcgg ccggcttccc ggtggtgtgg tcctccagct
     1621 tcgcggtctc cgccgcgcag cgggcggtgc ccgacgtgaa cctcctgacc atgaccgaga
     1681 acctggaggc cgccctcttc atcgaccgag cggtggcggc cccggtcatc gcggacgtcg
     1741 acaacggcta cggcaacgtg gtcaacgtcg tccgcaccgt gcgggagtac gagcgggccg
     1801 gtatcgcggg catctgcatg gaggacaacg tcttccccaa gaagtgcagc ctctacccgg
     1861 gcatgaagcg ggagctgctc tcggtggagg agttcgcggg caaggtgcgc gcctcggtcg
     1921 acacccgcca ggaccccgac ttcgtggtga tcgcgcgcgt cgaggccctc atcgcgggct
     1981 ggggcatgaa ggaggcgctc cgccgggccc acgcctacgc cgacgcgggc gcggacatgg
     2041 tgctgatgca ctccaagtcg aagaccgcgg ccgagatcct cgagttcatg cgtctctggg
     2101 accggccgac cccggtggtg gtggtgccga ccctctaccc gtcggtcacc ttcgaggagc
     2161 tcgagcaggc cggggtcaag atggtgatct gggcgaacca cgtgctgcgc ggcgcggtcc
     2221 agggcatgca gcagaccctc gcgaccctcg cccgcgagcg ccgcctcgac gcgctcgacc
     2281 cccacatcgt gccgctggag gagatctaca agcacgtggg ggtcgacgag ttcaaggcga
     2341 tcgaggacgc ctacctcccc gcctccgccg gccccgcccg cgcgatcatc atcgcggcgg
     2401 gctccggcaa gtcgctgctg ccgctcaccg aggaccggcc caagtgcatg ctcgacatca
     2461 agggccgcac catcctggag cgccagatcg acaccctgcg ggcctgcggg gtgcaggaca
     2521 tcgtggtggt gcgcggctac aagaaggaca tggtcacggt gccgggggtg cgctactacg
     2581 accacgaccg ctacgaggag accggcgagc tgggctcgct cttcgcggcc gagcgcgagc
     2641 tgcgcgggcc ggtgctgttc ctctactcgg acatcctgtt cgagcagacc gtgctcgaca
     2701 agctcctgcg cgcggagacc gactgcgcca tcgtcgtcga ccgcgcctgg gtggaccagc
     2761 gcgagcggct gctgccgctc gggaagccgc tcgacctggt ggtgaccgcc cgcacgcccg
     2821 aggccggcct gcgcgccctg cccggcgccc ccgacgaccg gctggtctcg gtcggccagc
     2881 gcatcgcccc cgagcgggcc accggcgagt tcatcgggct cgcgctcttc tccgagcggg
     2941 gcaccaccgc gatgcgcgag gcctaccagc ag
//
LOCUS       DATMCV010000355         3999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_485471, whole genome shotgun sequence.
ACCESSION   DATMCV010000355 DATMCV010000000
VERSION     DATMCV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485471"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VNU03_08280"
     CDS             complement(<1..965)
                     /locus_tag="VNU03_08280"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWP74498.1"
                     /translation="MVDVARGLERPWGLAFLPDGRLLVTERPGRLRIVTGGGLSAPLA
                     GVPAVFARGQGGLLDVALSPAFAQDRLVYLSFAEPGEGGAGTAVARGRLGPRGLEDTQ
                     VIWRQQPKVEGPNHWGSRLVFRPDGTLFVTLGERFAYRERAQDLSTTLGKIVRINPDG
                     SIPRDNPFVGRRGVLPEIWSYGHRNVQAATLDAQGRLWTVEHGPMGGDELNRPEAGKN
                     YGWPVITYGLDYSGARIGIGTSKPGMEQPVYYWDPVIAPSGATFYTGSAFPDWRGDLL
                     VGSLRPGGLVRLRLEHDRVVLEERYLAELDERIRDVRQGPDGGLYL"
     gene            complement(1085..2278)
                     /locus_tag="VNU03_08285"
     CDS             complement(1085..2278)
                     /locus_tag="VNU03_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWP74499.1"
                     /translation="MADVQFNPMDPAFVEDPYPTYHVLRSQDPVHHSPLGFWVLTRYA
                     DVIAMLRDPRLIKEPIAAFVAARFGMAVPPGLGLSMLDRDPPDHTRLRGLVSKAFTPK
                     ALESLRPRIQQIVDGLVDQVESRGEMDLVEEFAYPLPVVVICEMLGVPVQDHERFKHW
                     GLDIARGLDAIMLPPDSEVGKRSMIGRKALAEYFRELIAERRAAPRGDMLSALIAAEE
                     AGDKLDEDELLATCILLLVAGHETTVNLIGNGTLALLRHPDQLRRLREDPALIGSAVE
                     ELLRYDGPVQRTARIPSEDITIGTETIPKGEMVMPFLGAANRDPAQFPDPDRLDITRT
                     DNRHIAFGMGIHFCLGAPLARMEGQIAINTLLRRMPKLALASQQPEHRKSLTLRGLVS
                     LPVAF"
     gene            2355..>3999
                     /locus_tag="VNU03_08290"
     CDS             2355..>3999
                     /locus_tag="VNU03_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWP74500.1"
                     /translation="MATITGSEVLAQSLRSQGVDTMFYLMGGPMLETEAACIKLGIRA
                     IDTRHEQAAALAAHAWTRVTRRPGVAMGCSGPGAINLATGVANAFTDCAPLVAIGGSS
                     PRVYLGMEAFQEIDQVAIMKPITKWAERIYDARRIPDVVDTAFRQATSGRPGPVYIDM
                     PGDILGEKIEEEQLHYPGPWKPAPRSLGDPGAVREAIALLAKAERPVIIAGSGVWWSD
                     GAAALRAFVEATGIPFYTTPISRGLIPEDHELAFLNARSKAFTDADVVLAVGTRFNWV
                     IQFGRPPRFAADLKVIHVDINPTQLGHNRSVDVPIAGDARAVLDQLRAEAEGKISPKR
                     YAAWTGKLRTLDAEKGAEQDKSMSREETPIHPLRLCKEIRDFMRRDAILVVDGQEILN
                     FGRQAIPTYVAGHRLNSGAFGTMGVGLPFGVGAKVAKPDTQVLVLHGDGSYGINAMEI
                     DTAVRHKIPVVVVISNNGGWTADAPWTRPLPKPGRNLGHTRYDRVAQELGAHGEYVEK
                     PHDIRPALERAFASGKPAVVNVITDSKARAQTVRFSAYTT"
BASE COUNT          599 a         1440 c         1425 g          535 t
ORIGIN      
        1 aggtagagcc cgccgtccgg gccctggcgc acgtcccgga tccgctcgtc cagctcggcg
       61 agatagcgct cctcgagcac cacccgatcg tgctcgagcc ggagccgcac gagtccgccg
      121 gggcggaggg agccgaccag caggtcgccg cgccagtcgg ggaatgcgga gccggtgtag
      181 aacgtcgcgc ccgacggggc gatcaccggg tcccagtagt agacgggctg ctccatcccc
      241 ggcttcgagg tgccgatgcc gatgcgggcg cccgagtagt cgagcccgta ggtgatcacc
      301 ggccacccgt agttcttgcc ggcctccggc cggttcagct cgtcgccacc catcggaccg
      361 tgctccaccg tccacaggcg cccctgcgcg tcgagcgtgg cggcctggac gttgcgatgc
      421 ccgtacgacc agatctcggg cagcacgccg cgccggccga cgaagggatt atcgcgcggg
      481 atcgacccgt ccggattgat gcgcacgatc ttgccgagcg tcgtcgagag atcctgcgcg
      541 cgctcgcgat aggcgaagcg ctcgccgagg gtgacgaaga gcgtgccgtc gggccggaag
      601 accagccgcg agccccagtg gttcggcccc tccaccttgg gctgctgccg ccagatcacc
      661 tgcgtgtcct cgaggccgcg tggcccgagg cgcccgcgcg cgaccgcggt tccggcaccg
      721 ccctccccgg gctcggcgaa cgagagatag accagccggt cctgggcgaa ggccgggctg
      781 agcgcgacgt cgagcagccc gccctgcccg cgcgcgaaca ccgccggcac cccggcgagc
      841 ggcgccgaca gcccgccgcc ggtgacgatg cgcaggcgcc cgggccgctc ggtgacgagc
      901 aggcgcccgt cgggcaggaa cgcgaggccc cacggccgct cgagcccgcg cgccacgtcg
      961 accacgcggg cggcgagctt gagcggggcg ggcgtgggcg agcgcggcgc gtcttgaccc
     1021 caagcggggc cccacgcggg cccggccagc aggagggccg cggcgagaac gcgaccgcgc
     1081 gtggtcagaa cgcgaccggc agcgacacga gcccccgcag cgtgaggctc ttccggtgct
     1141 ccggctgctg gctcgcgagg gccagcttcg gcatccgccg cagcagggtg ttgatcgcga
     1201 tctggccttc catgcgcgcg agcggggcgc ccaggcagaa gtggatgccc atgccgaagg
     1261 cgatgtgccg gttgtcggtg cgggtgatgt cgagccggtc cgggtccggg aactgggcgg
     1321 ggtcgcgatt ggccgcgccc aggaagggca tcaccatctc gcccttggga atggtctctg
     1381 tcccgatcgt gatgtcctcg ctcgggatgc gcgcggtgcg ctgcaccggc ccgtcgtagc
     1441 gcagcagctc ctcgaccgca ctcccgatca gcgcggggtc ttcccgcagg cgtcgcagct
     1501 gatccgggtg acgcagcagc gccagggtgc cgttgccgat caggttcacg gtggtctcgt
     1561 gcccggccac cagcagcagg atgcaggtgg ccagcagctc gtcctcgtcg agcttgtcgc
     1621 ccgcctcctc cgccgcgatg agggcggaga gcatgtcgcc ccgcggggcg gcccggcgct
     1681 ccgcgatcag ctcgcggaag tactccgcga gcgccttgcg gccgatcatg ctccgcttgc
     1741 ccacctccga gtcgggcggc agcatgatcg cgtcgaggcc gcgcgcgatg tcgagccccc
     1801 agtgcttgaa ccgctcgtga tcctggaccg gcaccccgag catctcgcag atcacgacga
     1861 ccggcagcgg gtaggcgaac tcctccacca ggtccatctc gccccggctc tcgacctggt
     1921 cgacgagccc gtccacgatc tgctggatgc gcgggcgcag gctctcgagc gccttcgggg
     1981 tgaacgcctt gctgaccagg ccgcgcaggc gggtgtggtc gggcgggtcc cggtccagca
     2041 tggagaggcc gagcccgggc ggcaccgcca tcccgaagcg ggcggccacg aaggcggcga
     2101 tgggctcctt gatcaggcgg ggatcgcgca gcatggcgat cacgtccgca tagcgggtga
     2161 gcacccagaa gccgagcggg ctgtggtgga ccgggtcctg gctgcgcagc acgtggtagg
     2221 tcgggtacgg atcctccacg aacgccggat ccatcgggtt gaactgaacg tcggccatgt
     2281 tggtcccctc ccgcggccat tctacatctg ctagagtctc ggcaccccct cacagcggag
     2341 gaaaggacaa ggccatggcg acgatcaccg gctcggaagt gctggcgcag tccctcaggt
     2401 cgcagggtgt cgacacgatg ttctatctga tgggcggccc catgctcgaa acggaggcgg
     2461 cctgcatcaa gctcggcatc cgcgcgatcg acacgcgcca cgagcaggcc gccgcgctgg
     2521 ccgcccacgc ctggacgcgg gtgacgcgcc ggcccggggt ggccatgggc tgctcgggcc
     2581 ccggggccat caacctggcc accggggtgg ccaatgcctt caccgactgc gccccgctgg
     2641 tcgccatcgg cggatcgagc ccgcgcgtct acctgggcat ggaggccttc caggagatcg
     2701 accaggtcgc gatcatgaag ccgatcacca agtgggccga gcgcatctac gacgcccggc
     2761 gcatcccgga cgtggtggac accgcgttcc gccaggccac gtccggccgt cccggcccgg
     2821 tctacatcga catgccgggc gacatcctgg gcgagaagat cgaggaggag cagctccact
     2881 acccggggcc ctggaagccg gccccgcgca gcctgggcga tcccggcgcg gtgcgcgagg
     2941 ccatcgcgct gctcgcgaag gcggagcgtc cggtgatcat cgcgggcagc ggggtgtggt
     3001 ggtccgacgg cgctgccgcg ctccgggcct tcgtggaggc caccggcatc ccgttctaca
     3061 ccacgccgat ctcgcgcggc ctgatccccg aggatcacga gctggccttc ctgaacgcgc
     3121 gcagcaaggc cttcaccgat gccgacgtgg tgctcgcggt gggcacgcgc ttcaactggg
     3181 tgatccagtt cggccgcccg ccgcgcttcg cggccgacct caaggtgatc cacgtcgaca
     3241 tcaaccccac ccagctcggc cacaaccgct cggtcgacgt gccgatcgcg ggcgacgcgc
     3301 gcgcggtgct cgaccagctg cgcgcggagg cggagggcaa gatcagcccc aagcgctacg
     3361 cggcctggac cggcaagctg cggacgctcg acgccgagaa gggcgcggag caggacaaat
     3421 ccatgtcccg cgaggagaca ccgatccacc cgctgcggct ctgcaaggag atccgcgact
     3481 tcatgcggcg cgatgccatc ctggtggtgg acggccagga gatcctgaac ttcggccgcc
     3541 aggccatccc gacgtacgtg gccggccacc ggctgaactc gggcgccttc ggcacgatgg
     3601 gcgtgggcct gcccttcggg gtgggcgcga aggtcgccaa gcccgacacc caggtgctgg
     3661 tgctgcacgg cgacggctcc tacggcatca atgcgatgga gatcgatacc gcggtgcgac
     3721 acaagatccc ggtcgtggtg gtgatcagca acaacggcgg ctggaccgcc gacgccccgt
     3781 ggacccggcc gctgccgaag cccgggcgca acctcggcca cacccgctac gaccgggtgg
     3841 cccaggagct gggcgcgcac ggcgaatacg tggagaagcc gcacgacatc cgccccgcgc
     3901 tcgagcgcgc gttcgcctcg ggcaagccgg cggtggtcaa cgtcatcacc gactccaagg
     3961 cgcgggcgca gaccgtccgc ttctccgcct acaccacgt
//
LOCUS       DATMCV010000356         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_485519, whole genome shotgun sequence.
ACCESSION   DATMCV010000356 DATMCV010000000
VERSION     DATMCV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485519"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..929)
                     /locus_tag="VNU03_08295"
     CDS             complement(189..929)
                     /locus_tag="VNU03_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74501.1"
                     /translation="MYGQPFFDLQLQFARKVAALSGLPFADALLRYTNLYVRFGLGRD
                     FDPDHPAWREYVAGLPGAADTAEWTYRFYLTRPETEPPGIVQTCGCFSYARLGEDRVR
                     LHFRNAESAGGSALGSDRRSERLADLAALFEHVRRTVDGPVRVVGASWLYNLDAYRRL
                     FPDAYLATARVAARRFQHMPLWGQFLDRHGKVRESMARPFLDRLESHSSLDTLERCFP
                     LQVLTLEAPAPEFYEFYRIETAGTQARH"
     gene            complement(995..1684)
                     /locus_tag="VNU03_08300"
     CDS             complement(995..1684)
                     /locus_tag="VNU03_08300"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HWP74502.1"
                     /translation="MPKHVGIVGVSAEGAALCYRALCVEGAELFGRHGHPEVTLHTYS
                     LADYMRHIDAGDWPAVGELLLSSVAHLQKSGAELLICPDNTVHQAIDLVRDRSPLPWL
                     HIAEEVAAVAPGRGFRRVLVLGTRYLMESPVYPAKLGPRGIRHETPGPEDRARINALT
                     LDELVNGRFTDATRSYFSGIIADGRHRGCDSVALACTEFPLLIGEADSVLPILDSTRI
                     LARAALRAAMP"
     gene            complement(1700..>2001)
                     /locus_tag="VNU03_08305"
     CDS             complement(1700..>2001)
                     /locus_tag="VNU03_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP74503.1"
                     /translation="AVGSKLGVSDEKILALADYATSPLYDEAERTALEYADCMTISGR
                     EVGDELFARLRRLYDDDALVELTATIAWENASSKYNRALRIPSQGLWKRSPEPRS"
BASE COUNT          321 a          708 c          688 g          284 t
ORIGIN      
        1 cgtcggctca tcctggtagg ccaggtaggc caggatgggc gcgaggatgg gcagggcgcc
       61 ggcccagcgg tagtaggtga cctcggtcag ccgggcgttc atcatttccc ttctatcaca
      121 cccctgtccc gggcccgcca ctccgggtgc gagatcaggc gggacgggct cgacgcggca
      181 accggccgtc agtggcgcgc ctgggtcccg gcggtctcga tccggtagaa ctcgtagaac
      241 tccggcgcgg gagcctcgag cgtcagcacc tggagcggga agcaccgctc cagcgtgtcc
      301 agactggagt gactctcgag ccgatccagg aacggacgcg ccatgctctc cctgaccttc
      361 ccgtgtcgat ccaggaactg tccccacagt ggcatgtgct ggaatcggcg cgcggccacc
      421 cgagccgtcg ccaggtatgc gtcggggaac aagcggcggt acgcgtcgag attgtacagc
      481 cacgacgccc cgacgacccg caccggccca tccaccgtgc gtctcacatg ctcgaagagg
      541 gcggccaggt ccgcgaggcg ctcactccgc cgatcgctcc cgagcgcgga gcccccggcg
      601 ctctccgcgt tccggaagtg caggcggacg cgatcctcac ccagccgcgc gtaggagaaa
      661 cagccgcagg tctgcacgat acccggcggc tccgtctccg ggcgcgtcag gtagaagcga
      721 taggtccact ccgcggtgtc cgcggcgccc gggagcccgg cgacatactc ccgccaggcg
      781 ggatgatccg gatcgaagtc gcggcccagc ccaaagcgga cgtagagatt ggtgtatcgc
      841 aggagcgcat ccgcgaaggg caacccggac agcgcggcga ccttgcgcgc gaactgaagc
      901 tggaggtcaa agaacggctg accgtacatg gcgctcctcg cggcggcgac tcggcagctc
      961 caatctggcg gccagagtat cagatgccgg cgggtcaggg catcgccgcg cgcagcgcgg
     1021 cgcgcgcaag gatccgcgta gaatcgagga tcggcagcac cgagtccgcc tcgccgatca
     1081 gcaaggggaa ctcggtgcac gcgagcgcga ccgagtcgca gccgcggtgc cgcccgtcgg
     1141 cgatgatccc gctgaagtat gatcgcgtcg cgtcggtgaa ccggccgttc accagctcgt
     1201 cgagcgtcag cgcgttgatc cgcgcccggt cctccgggcc gggggtctcg tgccggatcc
     1261 ctcgtggccc gagcttcgcc gggtacaccg ggctctccat caggtaccgg gtcccgagga
     1321 ccagaacgcg ccggaagcca cgcccgggcg cgaccgcggc cacctcttcc gcgatgtgca
     1381 gccacggcag cggcgatcgg tcccgcacca ggtcgatggc ctggtggacg gtattgtccg
     1441 ggcagatcag cagctccgcg ccgctcttct gaaggtgcgc caccgacgag agcagcagct
     1501 cacccaccgc gggccaatca cccgcatcga tgtgccgcat gtagtccgcg agcgagtacg
     1561 tgtgcagggt gacctccgga tgcccgtgcc ggccgaagag ctcggcccct tccacgcaca
     1621 gcgcccggta gcagagggcg gccccctccg cgctcacccc gacgatcccg acgtgcttcg
     1681 gcacccggac gcggccggcc taggagcgcg gctcggggct ccgcttccac agcccctggg
     1741 acggaatccg cagggcgcgg ttgtacttgc tggaggcgtt ctcccaggcg atggtcgcgg
     1801 tcagctccac cagcgcgtcg tcgtcgtaga ggcggcgaag ccgggcgaag agctcgtccc
     1861 cgacctcgcg gccgctgatg gtcatgcagt cggcatattc gagcgcggtc cgctcggcct
     1921 cgtcgtagag cggactggtc gcgtagtcgg ccagcgcgag gatcttctca tcggacacac
     1981 ccagcttgct gcccacggca g
//
LOCUS       DATMCV010000357         4718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_154883, whole genome shotgun sequence.
ACCESSION   DATMCV010000357 DATMCV010000000
VERSION     DATMCV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154883"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..476)
                     /locus_tag="VNU03_08310"
     CDS             complement(144..476)
                     /locus_tag="VNU03_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP74504.1"
                     /translation="MTTPPVSLFVNASTVPWVERRPGVFWKTLWEDADGRHKAILMRY
                     EPGSVVPRHRHVGDEQIYVLEGSVADETGVCTAGNYARRPPGCIHTVTSPAGALVLAI
                     TSGPTEAV"
     gene            complement(494..1123)
                     /locus_tag="VNU03_08315"
     CDS             complement(494..1123)
                     /locus_tag="VNU03_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWP74505.1"
                     /translation="MMNPIARSRVYTLLLAAGLLLLTEVAGAAPGVPGVIVDPARTRG
                     PEAAPVTILEWSDFQCPFCGEVQPVLARLLAEFPQQVRLVFKDFPLRSHELAVPAALA
                     ARCASPEGRFWEYHDLLFVAQPAFARDELVGYARRLGLDVPRFTECLDAARFQEDVIA
                     DQREGRAAGVRATPTFFVNGRKIEGALPIEEFRDAVRRALREAGVAPPR"
     gene            1110..1481
                     /locus_tag="VNU03_08320"
     CDS             1110..1481
                     /locus_tag="VNU03_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWP74506.1"
                     /translation="MGFIIRVVVYTLALLLAAHVVPGISLSGLTSAVVAGLVLGIINA
                     VVRPILVVLTFPITLLTLGLFLLVLNAFCLWLVSVFVAGFHVTGFGPAFWGALLVSVV
                     SWILTALISDSGRVKAIHRRD"
     gene            complement(1482..2243)
                     /locus_tag="VNU03_08325"
     CDS             complement(1482..2243)
                     /locus_tag="VNU03_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWP74507.1"
                     /translation="MSDDRKLAMFMDFENIVRGVKEAQYKQFEIKLVLERLVEKGKIM
                     VKRAYADWNRYAEYKRPFHEHAIELIDVPQSRYSGKNSADIRMVVDAMDLAYAKQHVD
                     SFALVSGDSDFSPLVSKLRENDRYVIGLGVKSSSSELLVGNCDEFIFYEDLIRESKKT
                     TSLRGLPEKKAEAFAQLIEAIQALQRENKDTLWGSMVKQTMIRKNPAFNESYYGYSTF
                     SKLLEEAAKQKILTLERDAKSGTYIITSIEGDRAA"
     gene            2420..2635
                     /locus_tag="VNU03_08330"
     CDS             2420..2635
                     /locus_tag="VNU03_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74508.1"
                     /translation="MRAIRVLTLCAAALLLAVTGRATTPGDAAGRQVRVGQAAPEITG
                     GPWINSEPLSLAGLRGRVVMVEFWTYG"
     gene            2651..2962
                     /locus_tag="VNU03_08335"
     CDS             2651..2962
                     /locus_tag="VNU03_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWP74509.1"
                     /translation="MIPQLRDWHAKYGKQGLTIVGVHSPEFFWEKPLDKLTSATKDLG
                     VRYPVVQDNDFAIWRRYDNWAWPAAVIVDKKGVVRFTHVGEGAYAEMEQLIQRLLLEP
                     A"
     gene            2959..4464
                     /locus_tag="VNU03_08340"
     CDS             2959..4464
                     /locus_tag="VNU03_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWP74510.1"
                     /translation="MTGPRLPLLIFGVVFAAALVLAALGYLSLRRWEQAAERQLREQA
                     RDTALMAAEKVEMAVVRAEQDGIADIQAVALDPAFGPRAVEAWRSRHPLFDAVYLCGR
                     DGRVLYPVPSSPEDGLVIAALRGEISQGFWDRGGRRHLDVDGRVVLAAVLQGSPRGPL
                     LIGLRRDDRTLGRDVLGRALAATEGRSALVVVDASGRSVWASRPPESAAPVLTVAFGE
                     ALPAWRVALYEPAGPAPRDMVRRQAMTFMAAFALLLALIGTGLVATYRLQRRESEIAR
                     LKSDFVANVSHDLKTPLSLIRMFAETLEMDRVPDEARRREYYAVLTRESERLTRLIDN
                     VLDFSRIESGRQRYDIAPSPVEPVVHEVLESFRHPLHQQGFALEVSIAPDLPDVPLDA
                     EALKQALANLLDNAMKYSADRRKIAVTARREGDGVAVEVADEGIGIPLSERERIFEKF
                     YRVGRSETQGRRGSGVGLALVKHIVEAHGGRVTVDGGPGEGSRFTLHLPGA"
     gene            4472..>4718
                     /locus_tag="VNU03_08345"
     CDS             4472..>4718
                     /locus_tag="VNU03_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP74511.1"
                     /translation="MPRILIVDDEPEMVRGLEDNLRFEGYQTVAAPDGRRGLALALSE
                     APDLILLDVMMPGMSGWDLCRELRRRGLDVPVIMLTAR"
BASE COUNT          630 a         1616 c         1736 g          736 t
ORIGIN      
        1 gcctgaggcg ctcgtctcgt ggccccattg agtggccccg ggggatgcga gtcagcggct
       61 ccggggagga cccccgcccc gccccctgcc ccggaccccc accgggccag cacgtgagac
      121 cgccgacgcc gcgcccgcga cagctacacc gcttccgtgg gcccggaggt gattgcgagg
      181 acgagggcgc cggcggggct ggtcacggtg tggatgcagc cgggggggcg gcgggcgtag
      241 ttgcccgcgg tgcagacgcc ggtctcgtcg gccacgctgc cctcgaggac gtagatctgc
      301 tcgtcgccca cgtggcgatg gcgtgggacc acggagcccg gctcgtagcg catcaggatc
      361 gccttgtggc ggccgtcggc gtcttcccag agcgtcttcc agaagacgcc cggacggcgc
      421 tcgacccacg gcacggtgct cgcgttcacg aagaggctga ccggcggtgt cgtcacgaga
      481 gcgggtctcc ttgtcagcgg ggtggggcga cgccggcctc gcggagcgcg cgacggaccg
      541 cgtcgcggaa ctcctcgatg gggagcgcgc cctcgatctt ccggccgttg acgaagaacg
      601 tgggcgtggc gcgcaccccc gccgcgcgcc cctcgcgctg gtcggcgatc acgtcctcct
      661 ggaaccgggc cgcgtccagg cactcggtga agcgcggcac gtcgaggccc agccgccgcg
      721 cgtaccccac gagctcgtca cgggcgaagg cgggctgcgc gacgaagagc aggtcgtggt
      781 attcccagaa ccggccctcg gggctcgcgc agcgcgcggc gagcgccgcg ggcaccgcca
      841 gctcgtgaga ccggagcggg aagtccttga agacgagacg cacctgttgc gggaactcgg
      901 ccaggagccg ggccaggacc ggctggacct ccccgcagaa cgggcactgg aaatcgctcc
      961 actcgaggat cgtcaccggc gcggcctccg ggccccgggt ccgggccgga tcgacgatga
     1021 cgccgggtac gccgggcgcg gctccggcca cctcggtcag caggagcagg cccgcggcga
     1081 ggagcagggt gtagactcgg gaccgggcga tgggattcat catccgggtg gtggtgtaca
     1141 cgctggcgct gctgctggcc gcgcacgtgg tgccgggcat cagcctgtcc gggctcacct
     1201 ccgcggtcgt ggcggggctc gtgctgggca tcatcaacgc ggtggtgcgg cccatcctgg
     1261 tggtgctcac ctttccgatc accctgctga ccctcgggtt gttcctgctg gtgctgaacg
     1321 cgttctgcct gtggctcgtg tccgtgttcg tggccggctt ccacgtgacc gggttcgggc
     1381 cggccttctg gggcgccctg ctggtgagcg tggtgagctg gatcctgacc gcgctgatca
     1441 gcgacagcgg gcgggtgaag gccatccacc ggcgcgacta gctaggcggc gcggtcgccc
     1501 tcgatcgacg tgatgatgta ggtgccgctc ttggcgtccc gctcgagcgt caggatcttc
     1561 tgcttggccg cctcctccag gagcttcgag aacgtcgagt agccgtagta ggactcgttg
     1621 aaggcgggat tcttccggat catggtctgc ttcaccatgg agccccagag ggtgtccttg
     1681 ttctcccgct gcagggcctg gatcgcctcg atgagctggg cgaacgcctc cgccttcttc
     1741 tcgggcagcc cgcgcaggga cgtggtcttc ttcgactcgc ggatcaggtc ctcgtagaag
     1801 atgaactcgt cgcagttgcc caccaggagc tccgacgagg aggacttcac cccgaggccg
     1861 atcacgtagc ggtcgttctc ccggagcttc gagaccaggg gggagaagtc ggagtccccc
     1921 gacacgagcg cgaagctgtc cacgtgctgc ttggcgtagg ccaggtccat cgcgtcgacc
     1981 accatccgga tgtcggccga gttcttgccg ctgtagcggc tctgggggac gtcgatcagc
     2041 tcgatggcgt gctcgtggaa ggggcgcttg tactccgcgt accggttcca gtccgcgtag
     2101 gcccgcttga ccatgatctt gcccttctcg accagccgct ccaggaccag cttgatctcg
     2161 aactgcttgt actgcgcttc cttgaccccg cggacgatgt tctcgaagtc catgaacatc
     2221 gccagcttcc ggtcgtcact catgcgttcc atccttcccg ctgcaggttg ccccggcgtc
     2281 ctgtgctgcc gggcgggccc atgcccgccc gctcgccgat tctcattata ggcggccccg
     2341 ccggggggta ccggcgagaa ttcacagccg cgtgacatcg cggggccccg tccggctaga
     2401 ctctccctgg aggtggacca tgcgcgcgat ccgagtgctc acactgtgtg cggcggcgct
     2461 cctgctggcc gtcacgggcc gggcgaccac cccgggggac gcggcggggc gccaggtgcg
     2521 ggtggggcag gcggcccccg agatcaccgg cggcccctgg atcaatagcg agccgctctc
     2581 gctcgccggt ctgcgggggc gggtggtgat ggtggagttc tggacctacg gctgatacaa
     2641 ctgcaagaac gtgatcccgc agttgcggga ctggcacgcg aagtacggca agcaggggct
     2701 caccatcgtg ggcgtgcact ccccggagtt cttctgggag aagccgctcg acaagctcac
     2761 gagcgcgacg aaggatctgg gcgtccgcta cccggtggtg caggacaatg acttcgcgat
     2821 ctggcgccgc tacgacaact gggcgtggcc ggccgcggtc atcgtggaca agaagggtgt
     2881 cgtgcggttc acccacgtgg gcgagggggc ctacgcagag atggagcagc tgatccagcg
     2941 cctcctgctc gagccggcgt gaccggcccg cggctgccgc tcctgatctt cggggtggtc
     3001 ttcgccgcgg ccctcgtgct ggccgcgctc gggtatctct cgctccggcg ctgggagcaa
     3061 gccgcggagc ggcagctgcg cgagcaggcg cgcgacaccg ccctcatggc ggccgagaag
     3121 gtggagatgg cggtggtccg cgccgagcag gacggcatcg cggacatcca ggccgtcgcg
     3181 ctcgatcccg ccttcggccc ccgggcggtg gaggcgtggc ggagccgtca ccccctcttc
     3241 gacgcggtct acctgtgcgg tcgcgacggg cgcgttctct acccggtccc gtcgtcgccg
     3301 gaggacggcc tggtcatcgc ggcgctccgg ggcgagatct cccagggctt ctgggaccgg
     3361 ggcgggcggc gtcacctcga cgtggacggg cgcgtggtgc tggccgccgt gctccagggc
     3421 tcgccgcgcg gaccgctcct gatcggcctc cggcgcgacg accgcacgct gggccgcgac
     3481 gtgctcggcc gcgcgctcgc ggccaccgag ggccggagcg cgctggtggt ggtcgacgcg
     3541 agcggccggt cggtgtgggc ctcgcggccc cccgagagcg cggcgccggt cttgacggtg
     3601 gccttcgggg aggcgctgcc cgcctggcgg gtggcgctct acgagccagc cggcccggcc
     3661 ccgcgcgaca tggtgcggcg ccaagccatg accttcatgg ccgccttcgc gctgctgctg
     3721 gccctcatcg gcaccgggct ggtggcgacc taccgcctcc agcggcgcga gtccgagatc
     3781 gcgcggctca agtccgactt cgtggccaac gtctcccacg atctcaagac gccgctctcg
     3841 ctgatccgga tgttcgcgga gaccctcgag atggaccggg tgcccgacga ggcgcggcgg
     3901 cgcgagtact acgcggtgct cacccgcgag agcgagcggc tcacccgcct catcgacaac
     3961 gtgctcgact tctcccgcat cgagagcggg cggcagcgct acgacatcgc accgagcccg
     4021 gtggagccgg tcgtccacga ggtgctcgag agcttccgcc accccctcca ccagcagggc
     4081 ttcgcgctgg aggtgtcgat cgccccggat ctgcccgacg tgccgctgga cgcggaggcc
     4141 ttgaagcagg ccctcgccaa cctgctcgac aacgccatga agtactcggc ggaccggcgc
     4201 aagatcgcgg tcaccgcccg ccgggagggc gacggggtgg cggtggaggt ggccgacgag
     4261 gggatcggga tcccgctctc cgagcgcgag cgaatcttcg agaagttcta ccgcgttgga
     4321 cggagcgaga cgcagggacg tcggggaagc ggggtggggc tggcgctggt caagcacatc
     4381 gtcgaggccc acgggggccg ggtgacggtg gacggcgggc ccggcgaggg cagccggttc
     4441 acgctgcatc tcccgggcgc gtgaggacgc ggtgccacgc atcctgatcg tcgacgacga
     4501 gcccgagatg gtgcgcgggc tggaggacaa cctgcgcttc gagggctacc agaccgtcgc
     4561 ggcgccggac gggcgccggg gcctggccct cgccctgtcg gaggcgccgg acctgatcct
     4621 gctcgacgtc atgatgccgg gcatgagcgg ctgggacctc tgccgcgagc tgcggcggcg
     4681 cgggctcgac gtgccggtca tcatgctgac cgcgcggg
//
LOCUS       DATMCV010000358         4866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_154915, whole genome shotgun sequence.
ACCESSION   DATMCV010000358 DATMCV010000000
VERSION     DATMCV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154915"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VNU03_08350"
     CDS             complement(<1..854)
                     /locus_tag="VNU03_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74512.1"
                     /translation="MSNRVRHSLTRSFRSAGLAFAAAVVFAGHPAFAVAQTLAPSDVL
                     RIAQIEQVTNFDPALMSTQDMGVLIDNVYEGLIGTDRDTARLVPRLAEKWAIAPDGKS
                     ITFALRKNVKFHDGTALDADAVKYSIERVKTINKGPAVFIRGISAVDVVDKSTVRLRV
                     SENPRLALKAMALVMVVSPAALKAHEEGGDLGQKWIQRNSVGTGPYKLGEVKPNERQV
                     YTRFDDYWRGWTKGQVSRVEYIVQLDPAAARLMLERGDVDMTAIYSNDAIEPLRANPQ
                     VNVYLKEFP"
     gene            999..1496
                     /locus_tag="VNU03_08355"
     CDS             999..1496
                     /locus_tag="VNU03_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWP74513.1"
                     /translation="MGKALGALDLDIIRCLQDDARSSVVGIAGRLRVPESTVRHRLNR
                     LVKGGVVEFATLTNPLHMGYQVWVLIDIQTELRRTRAVAERVAEAPEVYWVGITTGTH
                     DLHVGAVFRSNEELLDFVTGRLSKIPGIVRTSTSSILELIKRSYTIRLPEPAIRKAPS
                     RRRKR"
     gene            1642..2280
                     /locus_tag="VNU03_08360"
     CDS             1642..2280
                     /locus_tag="VNU03_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWP74514.1"
                     /translation="MRDPYLRPSDLTRRAFLTTAGASAAAAALAGCAPASQPGVQRGA
                     AGAVADAPKTGAVAVTLRINGQEQRVSVDPRTTLLDCLRETVGLTGTKKGCDHGQCGA
                     CTVHVNGRRVNSCLSLALMHDGEEITTIEGLGSGLGAPEALHPMQAAFLARDGYQCGY
                     CTSGQIMSAVALLKEPCGRDDADVKEFMSGNICRCGAYPNIVAAIQQVRGNR"
     gene            2300..3301
                     /locus_tag="VNU03_08365"
     CDS             2300..3301
                     /locus_tag="VNU03_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWP74515.1"
                     /translation="MHAFDFMRPGDAEAAIRAAAQTKTAQQGADVRFLAGGTTLLDLM
                     KLNVETPARVVDINRLPFAKVEATPEGGLRIGATVRNSDLAYHPTVRRDYLVLSEAIL
                     AGASAQLRNMATTAGNLLQRTRCMYFRDTAMPCNKREPGSGCPAITGSNRTLAVLGAS
                     ESCIATNPSDMNVALAALEATVHVQGTKGTRAVPIGEFHLLPGQTPERETVLEPGDLI
                     THVTLPPPVAGSKQTYLKLRDRASYEFALASAAVVLTLASGRVTWARVALGGVGTKPW
                     RSPEAEAALVGQPAEAASFRRAAEAALRDARPQSENAFKIELAKRCLAHALHTATQS"
     gene            3317..>4866
                     /locus_tag="VNU03_08370"
     CDS             3317..>4866
                     /locus_tag="VNU03_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWP74516.1"
                     /translation="MATSSIGRDTPRVDGPLKVSGKAQYASDFHFPGMLYAMPVAATI
                     ANGRVTAIDSAVATKMPGVRAVFHRENIGKIFRSTFGPGFQGVTDERRPPFEDDVVRY
                     YGQYVALAVADTFEAARAAADAVRVTYAREEPNVATELAADDEADVVATSFGPRDRRQ
                     SQRGDADAAFAAAPVKLDQVYVTPAETHNPIELHATTAVWEGSTLTLYEGSQAVFNHR
                     GVIAQMFGLPVENVRVVTRFLGSGFGGKLWPWTHSSLAAAAARQLGRPVQLVVSRKMM
                     FQTVGHRARTQQRVRLGATAEGKLLSLQHDYVYHRSMLDAHHEDCGEATSFQYSVPNL
                     RVTFGRAKRHVGSACDMRGPGAVPGLYATEAAMNELAAQLRIDPLRLRVMNEPKIDEG
                     LGIPFSSRHQLECFELGAQKFGWSRRTPGVGSMTRDGLTLGWGMAGAAWIAARFPAEA
                     SVQLHDDGSARVACGTQDIGTGMYTILAQLASEKTGVPLGKVEVALGDTSLPAGPISG
                     GSMVTGSVV"
BASE COUNT          747 a         1683 c         1746 g          690 t
ORIGIN      
        1 gggaactcct tcaggtatac gttcacctgc gggttcgcgc gcaggggctc gatggcgtcg
       61 ttgctgtaga tggcggtcat gtcgacgtcg ccacgctcca gcatgagccg cgccgcggcg
      121 ggatcgagct ggacgatata ctccacgcgg gagacctggc ccttggtcca gccgcgccag
      181 tagtcgtcga agcgcgtgta gacctgccgc tcgttcggct tcacctcccc cagcttgtag
      241 gggccggtgc cgacgctgtt ccgctggatc cacttctggc cgagatcgcc gccctcctcg
      301 tgggccttga gcgcggcggg gctcacgacc atgaccagag ccatcgcctt cagggccagg
      361 cgcgggttct ccgagacgcg caggcgcacg gtggacttgt cgaccacgtc gaccgccgag
      421 atcccgcgga tgaacaccgc cgggcccttg ttgatcgtct tcacgcgctc gatcgagtac
      481 ttgaccgcgt cggcgtccag cgccgtcccg tcgtggaact tgacgttctt ccggagggcg
      541 aaggtgatgc tcttgccgtc gggtgcgatg gcccacttct cggcgagacg tgggacgagg
      601 cgggcggtgt cgcggtcggt gccgatcagg ccctcgtaca cgttgtcgat gaggacgccc
      661 atgtcctggg tcgacatcag cgcggggtcg aagttggtca cctgctcgat ctgcgcgatc
      721 cggagcacat ccgacggcgc cagggtctgc gcgacggcga acgccgggtg ccccgcgaag
      781 accacggccg ccgcgaaggc gaggccggcg cttcggaagg aacgggtgag agagtggcgg
      841 acgcggttcg acataacctc tcctccttcg atcggatggg acggatgcta tactcgccgc
      901 cgaggcgctg tcaacgcagt attgctgttt cagcaatgca agggcgtcga tgttgaggaa
      961 ttcgcaagat gagaccgacg ggacgacgga gaccggcaat ggggaaggcg ctgggggccc
     1021 tcgacctcga catcatccgg tgtctgcagg acgacgcgcg cagctcggtc gtggggatcg
     1081 cgggccggct tcgcgtgccg gagtccaccg tccgccaccg gctgaaccgg ctggtgaagg
     1141 ggggcgtggt agagttcgcc acgctgacga atcccctcca catgggatac caggtgtggg
     1201 tgctgatcga catccagacc gagctccgcc gcacgcgggc ggtggccgag cgagtggcgg
     1261 aagcgccgga ggtgtactgg gtcggcatca ccacgggcac ccacgacctc cacgtgggcg
     1321 cggtgttccg gtcgaacgag gagctcctgg acttcgtcac cggccgcctc tcgaagattc
     1381 cgggaatcgt gcgtacgtcc acctcgagca tcctcgagct gatcaagcgc agctacacga
     1441 ttcggctccc cgagccggcg attcggaagg cgccgagccg gcggcgcaag aggtgagcgg
     1501 cgcgcggccg acctgatcca gccgggcgca tacctgcaag ccacggcaca cgccttgccg
     1561 tcagtgtcct gttcttgcgg caaagcggag tggcgcgtca ccggagtcgt gcccgcggcg
     1621 tcacaagcgc acggaggatc tatgcgagac ccatacctgc ggccgtcgga cctgacccgg
     1681 cgggcgtttc tcaccacggc gggtgcttca gcggcggccg cggccctggc cggatgcgcg
     1741 ccggcgtcac agcccggcgt tcagcgcggg gcggccgggg cggtggcgga cgcgccgaag
     1801 accggcgcgg tcgcggtcac gctgcggatc aacggccagg agcaacgcgt ctccgtcgac
     1861 ccgcggacga ccctgctcga ctgcctccgc gagaccgtgg gcctcaccgg taccaagaag
     1921 ggctgcgacc acgggcagtg cggggcgtgc acggtgcacg tgaacgggcg ccgggtcaac
     1981 tcctgcctca gcctcgccct gatgcacgac ggggaggaga tcacgaccat tgaaggcctg
     2041 ggctcgggcc tgggcgcgcc ggaggcgctg cacccgatgc aggccgcgtt cctggcccgc
     2101 gacggctacc agtgcggcta ttgcacgtcg ggccagatca tgtcggcggt ggcgctgctc
     2161 aaggagccct gcgggcgcga cgacgcggac gtgaaggagt tcatgagcgg caacatctgc
     2221 cgctgcggcg cgtatccgaa catcgtggcc gcgatccagc aggtccgcgg aaaccgctag
     2281 cccgagcagg agcgatccca tgcatgcttt cgacttcatg cgtccgggcg atgccgaggc
     2341 cgcgatccgg gccgccgccc agacgaagac cgcgcagcag ggggcggacg tgcggttcct
     2401 cgcgggcggg acgaccctgc tcgacctgat gaagctgaac gtggagaccc cggcccgggt
     2461 ggtcgacatc aaccggctgc ccttcgccaa ggtcgaggcc acgcccgagg gcgggctccg
     2521 gatcggcgcc accgtccgga actcggatct ggcctaccac ccgacggtgc ggcgggacta
     2581 cctcgtgctg tcggaggcca tcctggccgg cgcgtcggcg cagctccgca acatggcgac
     2641 gacggccggc aacctgctcc agcgcacgcg gtgcatgtac ttccgcgaca ccgcgatgcc
     2701 gtgcaacaag cgcgagcccg gaagcggctg ccctgccatc accggcagca atcgcacgct
     2761 cgcggtgctg ggcgcgagcg agagttgcat cgccaccaac ccgtcggaca tgaacgtggc
     2821 gctggccgcc ctggaggcga cggtgcacgt gcaggggacg aaggggaccc gcgcggtccc
     2881 gatcggggag ttccatctcc tgcccggcca gaccccggag cgggagacgg tgctggagcc
     2941 gggcgacctg atcacccacg tcacgctgcc gccgcccgtc gcgggaagca agcagaccta
     3001 cctgaagctc cgcgatcgcg cgtcctacga gttcgccctc gcctccgccg cggtcgtgct
     3061 caccctcgcg agcggaagag tgacatgggc ccgcgtggcc cttgggggcg tgggcaccaa
     3121 gccgtggcgg tctcccgagg cggaggccgc gctcgtgggg cagccggccg aggcggccag
     3181 cttccgccgc gcggcggagg cggccctgcg cgacgcccgg ccgcagagcg agaacgcctt
     3241 caagatcgag ctggcgaagc gctgcctcgc gcatgcgctg cacaccgcca cgcagagctg
     3301 agcccaggga gcccacatgg cgacgtcatc gatcggccgc gacaccccgc gcgtggacgg
     3361 cccgctgaag gtgagcggga aggcccagta cgcctccgac ttccacttcc cgggcatgct
     3421 gtacgcgatg ccggtggcgg cgacgatcgc gaacggccgc gtgaccgcga tcgactccgc
     3481 cgtggccacg aagatgccgg gcgtgcgggc cgtcttccat cgagaaaaca tcgggaagat
     3541 cttccgctcc accttcgggc cgggcttcca gggtgtcacc gacgagcgcc gcccgccgtt
     3601 cgaggacgac gtggtccgct actacggcca gtacgtcgcg ctcgcagtgg cggatacctt
     3661 cgaggccgcc agagcggcgg ccgacgcggt acgggtcacc tacgctcgcg aggagccgaa
     3721 cgtggccacc gagctcgcgg cggacgacga ggcggacgtc gtcgccacct cgttcggccc
     3781 ccgcgatcgc cgccagagcc agcgcggcga cgcggatgcc gcgttcgccg ccgcgccggt
     3841 caagctcgac caggtctacg tcacgccggc cgagacgcac aaccccatcg agctgcacgc
     3901 cacgaccgcg gtctgggagg gctcgacgct gaccctgtac gaggggtcgc aggccgtctt
     3961 caaccaccgc ggcgtcatcg cccagatgtt cgggctgccg gtcgagaacg tccgggtcgt
     4021 cacccggttc ctgggctcgg gcttcggggg caagctgtgg ccctggaccc acagctcgct
     4081 cgccgccgcg gcggcgcggc agctcggccg gccggtgcag ctcgtggtca gccgcaagat
     4141 gatgttccag accgtcgggc atcgggcgcg cacccagcag cgggtgcgcc tcggggcgac
     4201 cgccgagggc aagctgctct cgctccagca cgactacgtc taccaccggt cgatgctcga
     4261 cgcgcaccac gaggactgcg gcgaggcgac ctcgttccag tacagcgtgc ccaacctccg
     4321 ggtgaccttc gggcgcgcca agcgccacgt cggctcggcg tgcgacatgc gcggccccgg
     4381 cgcggtgccc gggctctacg cgaccgaggc ggcgatgaac gagctggccg cgcagctgcg
     4441 gatcgatccg ctgcggctcc gcgtcatgaa cgagccgaag atcgacgagg gcctggggat
     4501 cccgttctcg tcgcgccacc agctcgagtg cttcgagctg ggcgcgcaga aattcggatg
     4561 gtcacgacgc acgcccggcg tcggctccat gacgcgcgac ggcctgaccc tcgggtgggg
     4621 tatggcgggg gccgcgtgga tcgcggcgcg cttccccgca gaggcgagcg tgcagctcca
     4681 cgacgacggc tcggcgcgcg tggcgtgcgg cacgcaggac atcggcaccg gcatgtacac
     4741 catcctcgcc cagctcgcct ccgagaagac cggggtgccg ctcggcaagg tggaggtggc
     4801 gctcggggac acctcgctac cggcgggacc gatctcgggc ggctcgatgg tgaccgggtc
     4861 ggtggt
//
LOCUS       DATMCV010000359         2689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_352700, whole genome shotgun sequence.
ACCESSION   DATMCV010000359 DATMCV010000000
VERSION     DATMCV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352700"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VNU03_08375"
     CDS             complement(<1..322)
                     /locus_tag="VNU03_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74517.1"
                     /translation="MLDESLDLAMVVGRIAPVRGWEPHFVGSLHELEQAVQAHGRPAL
                     TVVNLQPPLTAWELGQRLRGLGLESPVVVLGARGSEGAAPALAGVQWLERPAGEAELA
                     AALER"
     gene            complement(357..854)
                     /locus_tag="VNU03_08380"
     CDS             complement(357..854)
                     /locus_tag="VNU03_08380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWP74518.1"
                     /translation="MTEFMDTQTGVFHEDSFRHLLTREAGRATRYQDFFSVCILRPDV
                     PGDRQQIGEEIERALSEKIPEFVRATDLVGQLSSAIAVILLHTAGSDALRVAERIRAN
                     IEQVAFREGPEGRPQRLTVSVGKVSFPHDGHTDSILLSQALAYLEQATQRGGNQVTHS
                     TATPD"
     gene            complement(1134..2600)
                     /locus_tag="VNU03_08385"
     CDS             complement(1134..2600)
                     /locus_tag="VNU03_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP74519.1"
                     /translation="MTPGAILVVDGKAESRAALVRVVKEAGYSVLEAGTGAEALALLS
                     SLHPQLVLLDVDQLDANALDLARQVKLESADVPVVILSTTGERDLVVKAMRTGAYDFL
                     VKPVAAQALKDVLKAALAQTDAAPIVTSPGGNGSAGPQAPLDVELLFRNSERMRAVED
                     IVRRAADTNATILLQGESGTGKEMVARAIHYISDRRDKPFLKVNCASLPGDLLESELF
                     GHEKGSFTGAHRRKPGKFELAHRGTFLLDEIGEMPLGLQAKLLHVLQDGQFFRVGGSE
                     VITSDARLIAATNRNLASVMTTGMFREDLYYRLNVVAISIPPLRERREEIPVLVEYFM
                     ARFCRQYNRDALKISQATMRLLQDYSWPGNVRELENMIKRAVVLQTEALVHQEIALRS
                     EKPWAPKPEGAPSPAPSAAPPATVGSAIPEAEMGLKDIARRAAMAAEKAVIKEVLEKV
                     RWNRAEAARLLKISYKAMLYKIRQVGLDDRPERGSGRK"
BASE COUNT          417 a          955 c          892 g          425 t
ORIGIN      
        1 cccgctccag cgccgccgcc agctccgcct cccccgccgg ccgctccagc cactgcaccc
       61 ccgccagcgc tggcgccgcc ccctccgagc cccgcgctcc cagcaccacc accgggctct
      121 ccagccccag cccccgcagc cgctgcccca gctcccacgc cgtcagcggc ggctgcagat
      181 tcaccaccgt cagcgccggc cgcccgtgcg cctgcaccgc ctgctccagc tcgtgcaacg
      241 accctacgaa atgcggctcc caaccgcgaa cgggggcgat gcgcccgacc accatggcga
      301 gatccagcga ttcgtcaagc accaggagat tcacgcgaag tcgttccttc cgaagcctag
      361 tccggggttg ccgtgctgtg cgtaacctgg ttgccaccgc gctgcgtggc ctgctccagg
      421 taggcgagcg cctgcgacag cagaatcgaa tcggtatggc cgtcgtgcgg gaaggacacc
      481 ttcccgacgc tgaccgtgag ccgctgcggg cggccttccg ggccctcgcg gaatgccacc
      541 tgctcgatgt tcgcgcggat ccgctcggcc actcgcagcg catcgctccc cgcggtgtgc
      601 agcaggatga ccgcgatggc agacgagagc tggccgacca ggtccgtcgc ccggacgaac
      661 tccgggatct tctccgagag ggctcgctcg atctcctcgc caatctgttg ccggtcgccg
      721 gggacgtcgg gccgcagaat gcagaccgag aagaagtcct ggtaccgcgt agcgcggccc
      781 gcctcgcgag tcagcagatg gcggaatgag tcctcatgaa atacccctgt ctgggtatcc
      841 atgaattcgg tcacatgtcc ctcccttgct gtgacaggtt aactcccgat ctgggcgcgg
      901 ccaaccccgg gccggttccg gagggatccc tgagcaagtc ttgcgccacg ggcgcaagag
      961 tgatccgatc cagccggggc aggggagccc tatcgcgacg gtttcactgc gagttgcgcg
     1021 tgcgatacga gggaggacgt gaaggcgaaa cggacggcgg gggcgctacc tgcgacgggt
     1081 acgctaggag ttgaggaacg gacttaggta gtcaccgggg caaacgaagc aaatcatttc
     1141 ctacccgagc ctcgctcggg tcgatcgtcg agcccgacct ggcggatctt gtacagcatg
     1201 gccttgtagc tgatcttcag cagccgcgcc gcctcggccc ggttccatcg cactttctcg
     1261 agcacttcct tgatcaccgc cttctcggcc gccatcgccg cccggcgggc gatgtccttg
     1321 agccccatct ccgcctcggg gatggcgctg ccgaccgtcg ccggtggtgc ggccgaaggg
     1381 gcgggggacg gagcgccctc cggcttgggg gcccacggct tctcgctccg gagggcgatc
     1441 tcctggtgca cgagggcctc ggtctggagc accaccgccc gcttgatcat gttctcgagc
     1501 tcccggacgt tgccgggcca ggagtagtcc tggagcaggc gcatcgtcgc ctgggagatc
     1561 ttgagcgcgt cgcggttgta ttggcggcag aaccgcgcca tgaagtactc gacgagcacc
     1621 gggatctcct cgcggcgctc gcgcaggggc ggaatcgaga tcgccaccac gttcagccgg
     1681 tagtagagat cctcccggaa catcccggtg gtcatcaccg acgccaggtt ccggttggtg
     1741 gccgcgatga gccgcgcgtc cgacgtgatc acctcgctgc ccccgacgcg gaagaactgc
     1801 ccgtcctgca gcacgtggag cagcttggcc tgcagcccca gcggcatctc cccgatctcg
     1861 tccaggagga aggtgccccg atgggccagc tcgaacttgc ccggcttgcg gcgatgggct
     1921 cccgtgaacg aacccttctc gtggccgaac agctccgact cgaggaggtc acccggcagc
     1981 gaggcgcagt tgaccttgag gaacggcttg tcccgccggt cggagatgta gtggatggcc
     2041 cgggccacca tctccttgcc ggtgccgctc tctccctgca gcaggatggt ggcgttggtg
     2101 tcggcggccc gccgcacgat gtcttcgacc gcccgcatcc gctcgctgtt ccggaacagc
     2161 agctcgacgt cgagcggtgc ctggggaccc gcggacccat tgccgcccgg cgacgtgacg
     2221 atcggcgcgg catcggtctg ggccagggcc gccttcagga catccttgag ggcctgggcg
     2281 gcgaccggct tgaccaggaa gtcataggcc ccggtacgca tggccttgac cacgaggtcc
     2341 cgctcgccgg tcgtggagag gatgaccacg ggcacgtcgg cactctcgag cttgacctgc
     2401 cgggccaggt ccagggcatt cgcgtcgagc tgatcgacgt ccaggaggac cagctgggga
     2461 tgcaggctcg acaggagggc gagcgcctcg gcccccgtcc cggcctcgag gacggagtag
     2521 ccggcctcct tgacgacccg gaccagtgcg gcccgactct ccgccttgcc gtcgacgacg
     2581 agaatcgctc cgggagtcat ggggcggcga tcagacccgg gcggcgccgg gtgaaccagg
     2641 cccttgacga cggggacgcg ggtcccgata ctggcacccg gcgaagcca
//
LOCUS       DATMCV010000360         5930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_552252, whole genome shotgun sequence.
ACCESSION   DATMCV010000360 DATMCV010000000
VERSION     DATMCV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552252"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..970)
                     /locus_tag="VNU03_08390"
     CDS             complement(143..970)
                     /locus_tag="VNU03_08390"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWP74520.1"
                     /translation="MFRQRSGSSLCYRCGKLNRVDAAVCFYCGARRPGLWGFAPMVGR
                     LVGGLDFTGAVIAACAVLYVASILLDPAAAFRARSPFDMLSPSDSALVRLGMAGAAPW
                     AAGWWWTLLTGVYLHGSLLHIVFNMLWVRQLAPAVEQLFGRARLVVIYTAAGVLGFAL
                     SNLVGIRLTLGASGAVFGLLGAMVAYGRTRGGTFGVAVLRQYGQWALFIFIASFFLAG
                     VNNWGHAGGFAGGYLAALAFGHLERRPERGLDRLAALGAVVLTVLSFALAIWNGFLR"
     gene            complement(1014..2258)
                     /locus_tag="VNU03_08395"
     CDS             complement(1014..2258)
                     /locus_tag="VNU03_08395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWP74521.1"
                     /translation="MKRAHCLLSAAVLLALGVVGLAVPTREPAPPAIAEAPAPEPAAA
                     PEPAPPVEITLKRGETLETALRRAGLERAEAAAIVAGLRGQVNMRRLAPGERLSVKPA
                     APDGAPEITWTRSPAERYEIRSTDGGWTVAAVRPDVETRVAAIAGEVRDSLFASVERL
                     GETAGLTARLVSLFEWDFDFAADSLPGDRFRFLVEKRYVGDELLGYGDILVAQYASAG
                     RPMLTSVAFEEAGGRADYFDASGRSVKKMFLRAPLDFTRVTSGFSHARHHPILGGLRP
                     HLAVDYGAPVGTPVRAVADGVVTMAGWDGGFGLSLSLRHARGYETMYNHLSRLDVKRG
                     ERVRQRQIIGRVGTTGLSTGPHLDYRVRKAGVFVNPLGEKFIPGSPVPAHRRGAFTVH
                     VKALLERLDAQAPFPPRVPGRP"
     gene            complement(2332..3534)
                     /locus_tag="VNU03_08400"
     CDS             complement(2332..3534)
                     /locus_tag="VNU03_08400"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HWP74522.1"
                     /translation="MDLEFLHSLAQPATTKIVLCSLDGLGGLPEPRTRKSELETARIP
                     TLHALAGRSACGLLRHVGPGITPGSGPGHLGLFGYDPLRFPVGRGVLEALGIDFELQG
                     GDVAARGNFCTVDARGAITDRRAGRIATDVCVRLCERLRTIRLDGVQLLVEPVKEHRF
                     VLVLRGAGLSGRLSETDPQQLGAPPLPVSALAPEAERTAALVNAFVEAARPLLRDQAP
                     ANMLLLRGFDQRPHLPLFPDTFRLRAAAVASYPMYRGLSRLVGMDVLKTGGTFSEEID
                     TLEQQWEHYDFFFLHYKDTDKAGEDGDFAAKVAALERFDALLPRVLALRPDVLAISGD
                     HATPSILKGHGWQGVPALVASAYCGDDLVSRFTERDCASGSLGVMPAQDLMPLLMANA
                     LRFTKYGA"
     gene            complement(3525..4439)
                     /locus_tag="VNU03_08405"
     CDS             complement(3525..4439)
                     /locus_tag="VNU03_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP74523.1"
                     /translation="MAVRTFLHYALEVPDQTVGQRFYEDFGLVDGTGRSDAVRLRPER
                     LGRDTVLLYGGARKRLHHLCYGAPGEDFIRVRESLRRAGVREMDPPAGAPEGGVWIRD
                     PDGNAVNIRDEAPAAVPAEAPLTLNGPGYVQREVARGYPERGRRIAPRKLGHVLLFTP
                     DMERLMRFYTATLGMKLSDRSRGIIAFLRCNTDHHTLALLSSHAPGFHHASFQVGSVD
                     EIAMGAVHMAERGWQPGWGLGRHVIGSNFFYYLRDPWGSFSEYYYDLDYIPENCAWEP
                     RDFPPEDSLYVWGPPCPADFGENKEAWT"
     gene            complement(4453..5385)
                     /locus_tag="VNU03_08410"
     CDS             complement(4453..5385)
                     /locus_tag="VNU03_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWP74524.1"
                     /translation="MKIVRFSQNGHAPRLGCFLGQDRVADLEATATAYLAGRGVVRAA
                     TIAAALFPADSTRGFLEGGSASQDMLTAMMESARGGKFQPVAHPLNAVRLHAPIADPG
                     KFICIGLNYKDHAAETNNAPPKEPPVFPKWANAIQDPGEPILRPRGEKTLDWEVELGV
                     VIGRTARFVKKEQALDYVYGYTIINDASARDFQFHTSQWGAGKAGDTLAPVGPYIADR
                     AEIPDPHVLDLKTWVNGTLMQNGSTRNFIFDVGYIIQYLTNFMTLSPGDLIATGTPAG
                     VGFSRKPQVTLQPGDKVKLEITGLGTLENPVKDA"
     gene            5672..>5930
                     /locus_tag="VNU03_08415"
     CDS             5672..>5930
                     /locus_tag="VNU03_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74525.1"
                     /translation="MRVATKWLCGLLVAVMALTSPLLARVAVVRAAELSPETSPADAD
                     VSDGDRVGAGFLNVGYVPGKAIVCGLGTVASVGVVIITLGSA"
BASE COUNT          861 a         2008 c         2268 g          793 t
ORIGIN      
        1 ggagcggccg gacggcaccg cggtggtcaa tccgtcggcg gagacgcggc tgcgcccggg
       61 cgaccggctc cgcgtcttcg gcctacccga gcaggtcgac gcgttccgcg cagaggcgga
      121 tcgccccctc tgagccgccc ggtcagcgca ggaagccgtt ccagatcgcg agcgcgaacg
      181 agagcacggt gaggaccacc gccccgagcg cggcgagccg gtcgagcccg cgctcggggc
      241 gacgctcgag gtggccgaag gcgagcgcgg cgaggtagcc gcccgcgaag ccgcccgcgt
      301 ggccccagtt gttcacgccg gccaggaaga agctggcgat gaagatgaag agcgcccact
      361 gcccgtactg ccggagcacc gcgactccga aggtcccgcc ccgggtccgt ccgtaggcga
      421 ccatggcgcc gagcaggccg aagaccgcgc cggaggcgcc gagggtgagg cggatgccga
      481 ccaggttcga gagcgcgaac ccgagcacgc ccgccgcggt gtagatgacg acgaggcgcg
      541 cgcggccgaa gagctgctcg accgcgggcg cgagctgacg gacccagagc atgttgaaga
      601 cgatgtggag caggctgccg tggaggtaga cgccggtgag gagggtccac caccagccgg
      661 ccgcccacgg ggccgcgccc gccatgccga gccggacgag ggcgctatcg ctcggcgaca
      721 gcatgtcgaa ggggctccgg gcccggaagg cggccgcggg gtcgagcagg atcgacgcga
      781 cgtagagcac cgcgcaggcc gcgatgaccg ctcccgtgaa gtccagcccg cccacgaggc
      841 ggccgaccat cggagcgaat ccccagaggc cgggccggcg tgcgccgcag tagaagcaca
      901 ccgccgcgtc cacccggttg agctttccgc agcggtagca gaggctcgag ccggaccgct
      961 ggcggaacat ggacggaatg cctaccgcga cgggagagcg agcggccctg gggtcagggt
     1021 cggccgggga cgcgcggcgg gaagggggcc tgggcgtcga ggcgctcgag cagtgccttc
     1081 acgtgaaccg tgaaggcgcc ccgccgatgg gcgggcaccg gggagcccgg gatgaatttc
     1141 tcgccgagcg gattcacgaa gacgccggcc ttgcgcacgc ggtagtcgag gtggggaccg
     1201 gtggagagcc cggtggtgcc gacgcgcccg atgatctgcc gctggcgcac ccgctcgccc
     1261 cgcttcacgt cgagccggga gaggtggttg tacatggtct cgtagccgcg ggcgtggcgg
     1321 agcgagagcg agagcccgaa gccgccgtcc cagcccgcca tcgtcaccac cccgtcggcg
     1381 accgcccgca ccggggtgcc gaccggcgcc ccgtagtcga ccgcgaggtg cggacggagc
     1441 ccgcccagga tcggatggtg acgcgcgtgg gagaagccgg aggtgacgcg cgtgaagtcg
     1501 agcggggcgc gcaggaacat cttcttcacc gagcggccgg aggcgtcgaa gtagtccgcc
     1561 cgcccccccg cctcctcgaa ggccacgctg gtcagcatcg ggcgcccggc gctcgcgtac
     1621 tgggcgacca ggatgtcgcc gtagcccagg agctcgtcgc cgacgtaccg tttctccacc
     1681 aggaagcgga agcggtcgcc cgggagcgag tccgccgcga agtcgaagtc ccactcgaac
     1741 aggctgacca ggcgcgcggt caggccggcg gtctccccga ggcgctccac gctcgcgaag
     1801 agcgagtcgc ggacctcgcc cgcgatcgcc gccacgcggg tctcgacgtc cgggcgcacc
     1861 gccgcgaccg tccagccgcc gtcggtcgag cggatctcgt agcgctccgc gggcgagcgg
     1921 gtccaggtga tctcgggggc gccgtcggga gccgccggct tcacgctgag ccgctcgccg
     1981 ggcgcgagcc gccgcatgtt cacctggccg cgtaggccgg ccacgatggc ggcggcctcc
     2041 gcgcgctcga gcccggcgcg gcgcagcgcg gtctcgaggg tctcgccgcg cttgagggtg
     2101 atctcgaccg gcggcgcggg ctccggcgcc gcggctggct cgggggccgg ggcctcggcg
     2161 atcgcgggag gggcgggctc ccgggtgggg acggcgaggc cgaccacgcc tagggcgaga
     2221 aggaccgcgg cggagaggag gcagtgcgcc cgcttcatga ctctgcggtc aggataccgc
     2281 gggaggattg tgctcccaac ctaaaccgcc ccggtgacgg ggcccgccgc cctacgcccc
     2341 gtacttcgtg aagcgaagcg cgttcgccat cagcaggggc atcaggtcct gggcgggcat
     2401 caccccgagc gagccgctcg cgcaatcccg ctcggtgaag cggctgacca ggtcgtcgcc
     2461 gcagtacgcg cttgcgacga gcgcgggcac cccctgccag ccgtggccct tcaggatgga
     2521 cggggtcgcg tggtcgcccg agatggcgag cacgtccggg cgcagggcga gcacgcgcgg
     2581 cagcagcgcg tcgaagcgct cgagcgcggc gaccttggcc gcgaagtcgc cgtcctcgcc
     2641 ggccttgtcg gtgtccttgt agtgcaggaa gaagaagtcg tagtgctccc actgctgctc
     2701 gagcgtgtcg atctcctccg agaacgtccc gccggtcttg agcacgtcca tgccgaccag
     2761 gcgcgagagg ccgcggtaca tcgggtacga cgcgaccgcg gcggcccgca gccggaacgt
     2821 gtcggggaag agcggcaggt ggggccgctg gtcgaagccg cgcaggagca gcatgttcgc
     2881 gggggcctgg tcgcggagga gcggccgcgc cgcctccacg aaggcgttga ccagcgccgc
     2941 ggtccgctcc gcctccgggg ccagcgcgga gacgggtagc ggcggcgccc cgagctgctg
     3001 tggatccgtc tcggagaggc ggccggacag acccgcgccc cgcaggacga gcacgaagcg
     3061 gtgctccttg accggctcca ccaggagctg caccccgtcg aggcggatcg tccgcagccg
     3121 ctcgcagagt cgcacacaga cgtcggtggc gatgcgtccc gcccgccggt cggtgatagc
     3181 gccgcgcgcg tcgacggtgc agaagttgcc ccgcgcggcc acgtccccgc cctgcagctc
     3241 gaagtcgatg ccgagcgctt cgaggacccc gcgtcccacc gggaagcgga ggggatcgta
     3301 gccgaagagg cccaggtggc cgggcccgct gccgggggtg atgccgggcc cgacgtggcg
     3361 cagcaggccg caggccgagc gcccggcgag ggcgtgcagc gtcgggatcc gcgcggtctc
     3421 gagctcgctc ttgcgggtgc gcggctcggg caggccgccg aggccgtcga gggagcagag
     3481 cacgatcttg gtggtggcgg gctgggccag ggagtggagg aactctaggt ccatgcttcc
     3541 ttgttctcgc cgaagtcggc cgggcacggc gggccccata cgtagaggga atcctcgggc
     3601 gggaagtccc gcggctccca cgcgcagttc tccgggatgt agtcgaggtc gtagtagtac
     3661 tcgctgaagc tgccccacgg gtcgcggagg tagtagaaga agttcgagcc gatcacgtga
     3721 cgcccgaggc cccagcccgg ctgccagccc cgctcggcca tgtgcaccgc ccccatcgcg
     3781 atctcgtcca cgctgcccac ctggaacgac gcgtggtgga agccgggggc gtgcgaggag
     3841 agcagggcca gggtgtggtg gtcggtgttg cagcgcagga acgcgatgat cccccgggag
     3901 cgatcggaga gcttcatgcc gagcgtcgcg gtgtagaagc gcatgaggcg ctccatgtcg
     3961 ggggtgaaga gcagcacgtg cccgagcttg cgcggggcga tgcgccggcc ccgctccggg
     4021 tagccgcgcg cgacctcgcg ctgcacgtag ccggggccgt tgagggtgag cggcgcctcg
     4081 gccggcacgg cggcgggcgc ctcgtcgcgg atgttgaccg cattgccgtc gggatcgcgg
     4141 atccagacgc cgccctccgg agcgcccgcg ggcggatcca tctcgcgcac gccggcgcgc
     4201 cgcagcgact cgcgcacccg gatgaagtcc tcgcccgggg cgccgtagca gagatggtga
     4261 aggcgcttcc gcgcgccccc gtagagcagc acggtatcgc ggccgagccg ctcgggacgc
     4321 aggcgcaccg cgtcgctccg gccggtgccg tccacgaggc cgaagtcctc gtagaagcgc
     4381 tggccgacgg tctggtcggg cacctccagc gcgtagtgga gaaaggtccg gaccgccatg
     4441 gcgggcggcc ggctaggcgt ccttcaccgg gttctcgagc gtgccgaggc cggtgatctc
     4501 gagcttgacc ttgtcgcccg gctggagcgt gacctgcggc ttgcgcgaga agcccacccc
     4561 ggcgggggtg ccggtggcga tgaggtcgcc cggcgagaga gtcatgaagt tggtcaggta
     4621 ctggatgatg tagcccacgt cgaagatgaa gttgcgggtg gagccgttct gcatgagcgt
     4681 gccgttcacc caggtcttga gatcgagcac gtgcgggtcc gggatctcgg cgcggtccgc
     4741 gatgtacggg ccgaccgggg ccagggtgtc gcccgccttg cccgcgcccc actggctcgt
     4801 gtggaactgg aagtcgcgcg cgctcgcgtc gttgatgatg gtgtagccgt agacgtagtc
     4861 gagggcctgc tccttcttca cgaagcgggc ggtgcggccg atcaccacgc ccagctccac
     4921 ctcccagtcg agcgtcttct ccccgcgggg ccgcaggatc ggctcgcccg gatcctggat
     4981 cgcgttggcc cacttcggga agaccggcgg ctccttcggc ggcgcgttgt tggtctcggc
     5041 cgcgtggtcc ttgtagttga ggccgatgca gatgaacttg cccggatccg cgatcggcgc
     5101 gtgcagccgc accgcgttca ggggatgggc caccggctgg aacttgccgc cccgcgcgct
     5161 ttccatcatc gcggtcagca tgtcctggct cgcgctgccc ccctcgagga agccgcgggt
     5221 gctgtcggcc gggaacagag ccgccgcgat ggtggccgcg cgcaccacgc cccgacccgc
     5281 gaggtatgcg gtcgcggtcg cctcgaggtc ggcgacgcga tcctgaccga ggaagcagcc
     5341 gaggcgggga gcgtgaccat tctgcgagaa gcggacgatc ttcatggaga gtctccttgc
     5401 ggtgagggcc ggtctcggct aggccccgtt ggccagcacg cagagagtct acgatcccgg
     5461 tccggcgatg tcgagggggg tgccatggag ggcaaggggg tgattcgact ggggtttggg
     5521 tgtgacgcat cgcgttgccg atgccccgac gggtccgatc gtggcgccga ccggcgggta
     5581 ccgctcatct aggtcaggat attcaattcg tgtaccttag atacgtcaga tcagttgggt
     5641 aaccgcactc gatccggaga ggagatacac catgcgggtt gccacgaagt ggttgtgcgg
     5701 cctgctcgtc gcggtcatgg cgctgacgag cccgctgctg gcgcgggtgg ccgtggtccg
     5761 ggctgccgag ctctcgcccg agacctcccc ggccgacgcg gacgtgtccg acggcgatcg
     5821 ggtgggcgcg gggttcctca acgtcggcta cgtgcccggc aaggccatcg tctgcggact
     5881 cggcaccgtc gccagtgtcg gggtcgtgat catcacgctc ggcagcgcct
//
LOCUS       DATMCV010000361         6246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_620000, whole genome shotgun sequence.
ACCESSION   DATMCV010000361 DATMCV010000000
VERSION     DATMCV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620000"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VNU03_08420"
     CDS             complement(<1..651)
                     /locus_tag="VNU03_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74526.1"
                     /translation="MRRLVRLSCRRPAVTVAISLLLAAVAVVYTMQALTFKTSTRSLL
                     PQNAGFVVRYAEYSKEFGELEDIVVVVEAGSFEGARAYAARLTDELESGPVKFHRIAY
                     RIDPKRFEGRQLLYISTPELREIRDKIFDHQEFMESFAGDPSLARLLEGVNTQMAAAF
                     VSNLFDIGLQDKDLPVDTRFLRVLLDQMASRLERPGPYRSPWGTLFSFGEDPPADAG"
     gene            complement(721..1794)
                     /locus_tag="VNU03_08425"
     CDS             complement(721..1794)
                     /locus_tag="VNU03_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HWP74527.1"
                     /translation="MGSVRVAVIGVGNCASALVQGIHYYKGVADDGFIPGLMRPRLGG
                     YHVGDIEFSAAFDIDANKVGLDLSEAIWAKPNNTVRFGEVPRLDVPVQRGMTHDGLGH
                     YLSQIITKASGTTADITQILRETRTDVVVNFLPVGSEMATKWYVEQVLDAGCGFVNCI
                     PVFIARENYWRRRFEDRGLPIVGDDVKSQVGATIIHRILTKLFMDRGVRLERTSQLNV
                     GGNTDFYNMLERERLASKKISKTDAVRSQLAHDMADDNIHIGPSDYVPWLQDRKWAHI
                     RLEGRSFGDQPINVELKLEVWDSPNSAGVVIDAIRSCKIAMDRGIKGALLAPSAYLMK
                     SPPEQWSDEVARTRLEKFIGGAD"
     gene            complement(1877..2878)
                     /locus_tag="VNU03_08430"
     CDS             complement(1877..2878)
                     /locus_tag="VNU03_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HWP74528.1"
                     /translation="MSRWVRPLLVLTGLAVVAYLVAQIGPAAIWSSFVTLGWRLPLVL
                     AFPYALAALVDTIAWRLLLPGPVPLSLVFRARLAGEAVNLATPTMAVGGEPVKALLLR
                     PRVSLLDGLVSVIADKTTIVVGQIIFLAGGLLAARTALPPSSRLVPIMTGLLAVEILA
                     IGGFALVQASGGLGGGGRLLGKIGARVGLGGERYLQGLGDLDRALAGLYRHGRRRLMA
                     SALVHSVAWATGGLEIYIVLSLLGAPISLGTALIIESFAGGVKFASFMVPASLGALEG
                     GYVAFFEAFGLTATAGLSFVLVRRLREMAWAGLGFLALGVSQRAHPPLPPDERERAA"
     gene            complement(2878..4095)
                     /locus_tag="VNU03_08435"
     CDS             complement(2878..4095)
                     /locus_tag="VNU03_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWP74529.1"
                     /translation="MVRALLYLPDAAADRLAATPVAGRTLAVRAMVAALRAGASRIAV
                     PSTLRDPAVERALHRMPALADALEWLDPGADAPADGPAARWLLLPASALIHASGLEGL
                     LAAPAGRPAVLAGSAGGSAPVALLPATLPATPWPSPLWFNGLWKDLAAGRPVGPELAR
                     WLREAGAVPLAVTGPYVDVRDPADLVRAQAALEARLSIPGDSGMDRLLHRRCSVWITR
                     FLVRTDLTPNHVSLASLAIGLAAIWCFWRATPASAFAGVLLYALACIVDHADGELARL
                     TFQESRFGASLDWTIDTLIHAGLVLGIGLTAGGPAMLVVGLLGAAGVTLSALFARYLP
                     HEIEIGPTVGGVLRNIANRDLFYLLLLGAATLRLLAPGLVSVAAVLVAVGSQAYWVGC
                     LTRIRRLGRERAV"
     gene            complement(4097..4837)
                     /locus_tag="VNU03_08440"
     CDS             complement(4097..4837)
                     /locus_tag="VNU03_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HWP74530.1"
                     /translation="MKAVILAAGVARRLAPLTDHTHKCLLPVGGRSLLDRMLDALSAA
                     GVDEAVLVLGHCEDQVRRAAGPARGAMRIGYVVNPDYQKGSILSLLCARDTLLRDSTL
                     IMDADVLFPDRFLRGLIDSPAESALLLDRGFQDTGEEVKLYAVGDRVIALGKKFVPQA
                     WDVVGEGVGFFKCSAGHAPEYLRLMEESVRETGGANEYEDALHRLLARVPVGWVDVTG
                     LPWTEVDFAEDLRRAETQVLPKIERLEP"
     gene            complement(4834..6012)
                     /locus_tag="VNU03_08445"
     CDS             complement(4834..6012)
                     /locus_tag="VNU03_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWP74531.1"
                     /translation="MGGDQGLTGPALAGLLEAQGYDFFTGVPCSMIEGLIATLETHPR
                     LPYVPAVREDVAVGLAAGAWLAGRRPMVLMQNSGLGTSMNALVSLSLMYRLPALLLVT
                     WRGHRGKDAPEHLLMGEISPALLELLRIPHRILSAETIADDVAWGRAESERLSQPVAL
                     LLPPGILDTASHAAAASAPAGAPVSRATSQDDPIPAPRISRFEALRAAVAPLTAEPVV
                     HANGYICRESFGVKDRPENFYMIGSMGMASAIGLGVALAVPDRSTVVFDGDGNLLMSL
                     GILPMIGGGPVMGRQRPANLVHVVFDNALYGSTGNQASPSRAVGLHRIARAAGYERSV
                     AVAGADEIRDAVTTALCGGGPSFILARVTAEEQPAPRIPYPPEEIRDRFRASFGRERR
                     "
     gene            complement(5991..>6246)
                     /locus_tag="VNU03_08450"
     CDS             complement(5991..>6246)
                     /locus_tag="VNU03_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HWP74532.1"
                     /translation="GEFIGLALFSERGTAAMREAYQQRMAAGTGPFHEADRPERGAFT
                     DLLQELVQRGETVACVDVYKGWLEVDTFEDYQRAWAAIKA"
BASE COUNT          963 a         2198 c         2316 g          769 t
ORIGIN      
        1 gcccgcgtcg gcgggcgggt cctcgccgaa ggagaagagg gtgccccagg gcgaccggta
       61 ggggccgggc cgctcgagcc ggctcgccat ctggtcgagc agcacgcgca ggaagcgcgt
      121 gtccaccggg aggtccttgt cttgcaggcc gatgtcgaag aggttcgaca cgaaggcggc
      181 cgccatctgg gtgttgacgc cttccagcag ccgggccagg ctcggatcgc ccgcgaagct
      241 ctccatgaac tcctggtggt cgaagatctt gtcgcggatc tcgcgcagct cgggggtcga
      301 gatgtagagg agctggcgcc cctcgaaccg cttgggatcg atccggtagg cgatgcggtg
      361 gaacttgacc ggcccgctct cgagctcgtc ggtgaggcgc gccgcatagg cgcgggcccc
      421 ctcgaaggag ccggcctcca ccaccaccac gatgtcctcg agctcgccga actccttgga
      481 gtattccgcg tagcgcacca cgaagcccgc gttctggggc agcagcgagc gggtggaggt
      541 cttgaaggtg agggcctgca tggtgtagac gaccgccacc gccgcgagca gcagggagat
      601 cgcgacggtg accgcggggc gtcggcaaga cagccggacc agccggcgca acaggcggcc
      661 ggctcgggag atcgcgacca ttggcagtgc ggggagtggt gcgggaggtg cgcggcgggc
      721 ctagtccgcg cccccgatga acttttccag gcgcgtccgc gccacctcgt cggaccactg
      781 ctcgggcggg gacttcatga gataggcgga cggcgcgagc agcgctccct tgatgcctcg
      841 atccatcgcg atcttgcagg agcggatcgc gtcgatgacc accccggccg agttggggga
      901 atcccacacc tcgagcttca gctccacgtt gatgggctgg tcgccgaagc tgcggccctc
      961 caggcggatg tgggcccact tgcgatcctg gagccacggc acgtagtcgc tcggcccgat
     1021 gtggatgttg tcatcggcca tgtcgtgggc gagctgggag cgcaccgcgt cggtcttgga
     1081 gatcttcttg gaggcgaggc gctcgcgctc cagcatgttg tagaagtcgg tgttgccgcc
     1141 cacgttcagc tggctggtcc gctccagccg caccccccgg tccatgaaga gcttggtgag
     1201 gatgcggtgg atgatggtgg cccccacctg ggacttcacg tcgtcgccca cgatcggcag
     1261 gccccggtcc tcgaagcgcc ggcgccagta gttctcccgg gcgatgaaca ccgggatgca
     1321 gttgacgaac ccgcagcccg cgtcgagcac ctgctccacg taccacttgg tggccatctc
     1381 gctgcccacc ggcaggaagt tcaccaccac gtcggtgcgc gtctcgcgca ggatctgggt
     1441 gatgtccgcg gtggtgcccg aggccttggt gatgatctgc gagaggtagt ggccgagccc
     1501 gtcgtgggtc atgccccgct gcaccggcac gtcgaggcgc gggacctccc cgaagcgcac
     1561 cgtgttgttc ggcttggccc agatcgcctc ggacaggtcg agccccacct tgttggcgtc
     1621 gatgtcgaag gcggccgaga actcgatgtc gcccacgtgg tagccgccga ggcgcgggcg
     1681 catgaggccc gggatgaagc cgtcgtcggc gacgcccttg tagtagtgaa tcccctggac
     1741 gagagcggac gcgcagttac cgacgccgat gaccgcgacg cgaacagacc ccacggacgc
     1801 ctcctcgaga atcgccgccg ctgacggcgg cacaaaaggt tttcacgaag tctagcacag
     1861 ggtcagcggg cccgcctcaa gctgcgcgct cccgctcgtc gggcgggagc ggcgggtggg
     1921 cgcgctggga gactcccagg gccaggaagc cgaggccggc ccacgccatc tcccgcagcc
     1981 ggcgcaccag cacgaaggag agtccggcgg tggcggtgag gccgaaggcc tcgaagaagg
     2041 ccacgtagcc gccctcgagg gcgccgagcg aggcgggcac catgaagctg gcgaacttca
     2101 cgcctcccgc gaaggactcg atgatcagcg cggtcccgag cgagatcggc gcccccagca
     2161 gggagaggac gatgtagatc tcgagcccgc cggtcgccca cgcgaccgag tggaccaggg
     2221 ccgaggccat gaggcggcga cgcccgtggc gatagagccc ggcgagcgcc cggtcgagat
     2281 cgcccagccc ctgcaggtag cgctcgccgc ccaggccgac ccgcgcgccg atcttgccga
     2341 gcagccgtcc gcccccgccg agccccccgc tggcctgcac cagcgcgaag ccgccgatgg
     2401 cgaggatctc gaccgcgagc aggccggtca tgatcggcac cagccggctg gacggcggga
     2461 gcgcggtgcg ggcagccagc aggcccccgg cgagaaagat gatctgcccg accacgatgg
     2521 tggtcttgtc cgcgatgacc gacacgaggc cgtcgaggag ggagacccgc ggccggagga
     2581 gcagggcctt caccggctcg ccccccaccg ccatcgtcgg ggtggccagg ttcaccgcct
     2641 ctccggccag ccgcgcgcgg aagaccaggg agagcggcac cgggccgggt aggagcaggc
     2701 gccaggcgat cgtgtcgacc agggcggcca gcgcgtaggg gaaggcgagc accagcggca
     2761 gccgccaccc gagcgtgacg aacgacgacc agatcgcggc gggcccgatc tgcgcgacga
     2821 ggtaggcgac caccgccagc ccggtcagca ccagcagcgg gcggacccag cgcgacacta
     2881 gacggcgcgc tcgcgcccca gccgccggat gcgcgtgagg cagccgaccc agtacgcctg
     2941 cgagcccacc gccaccagca ctgcggccac gctcaccagc ccaggagcca gcaggcgcag
     3001 cgtcgcggcg ccgagcagca gcaggtagaa gaggtcgcgg ttggcgatgt tccggagcac
     3061 gccgcccacg gtggggccga tctcgatctc gtgcggcagg taccgcgcga agagcgcgct
     3121 gagcgtcacg ccggccgcgc ccagcaggcc caccaccagc atcgcggggc cgcccgcggt
     3181 gagcccgatg ccgagcacca ggccggcgtg gatgagggtg tcgatcgtcc agtcgaggct
     3241 cgccccgaag cgcgactcct ggaaggtcag gcgggccagc tcgccgtcgg cgtgatcgac
     3301 gatgcaggcg agcgcgtaga ggaggacgcc ggcgaaggcg ctcgccggcg tggcccgcca
     3361 gaagcaccag atggcggcga ggccgatggc caggctcgcg aggctcacgt ggttcggggt
     3421 cagatccgtg cggacgagga agcgggtgat ccagacggag catcgacggt gaagcaaccg
     3481 gtccatgccg gagtcgcccg ggatcgagag cctggcctcg agcgcggcct gggcgcggac
     3541 caggtccgcc gggtcgcgca catcgacgta ggggcccgtg accgcaaggg gcaccgcgcc
     3601 cgcctcgcgc agccagcgag ccagctcggg gcccaccgga cggcctgcgg ccaggtcttt
     3661 ccagagaccg ttgaaccaga gggggctggg ccagggcgtc gcgggcagag tcgcgggcag
     3721 cagcgccacc ggggcgctcc ccccggccga tccggccagc acggcgggcc ggccggcggg
     3781 cgccgcgagc aggccctcca gcccgctcgc gtggatcagc gcggaggcgg ggagcagcaa
     3841 ccagcgggcg gccggaccgt ccgcgggcgc gtcggcgccc gggtcgagcc actcgagcgc
     3901 gtcggcgaga gcgggcatcc ggtgcagcgc gcgctccacc gccgggtccc gcagggtcga
     3961 gggcaccgcg atgcgggagg cgccggcgcg gagggccgcc accatcgcgc gcaccgcgag
     4021 ggtgcgcccg gcgaccggag tcgcggctag tcggtcggcg gccgcgtcgg ggagatacag
     4081 gagcgcgcga accacatcag ggctcgagtc gctcgatctt ggggaggacc tgggtctcgg
     4141 cccggcgcag gtcctccgcg aagtccacct cggtccacgg caggccggtc acgtccaccc
     4201 agcctaccgg cacccgggcc agcaggcgat ggagcgcgtc ctcgtactcg ttggcgccgc
     4261 cggtctcccg gacggactcc tccatcaggc ggagatactc gggcgcgtgg cccgcgctgc
     4321 acttgaagaa gccgactccc tccccgacca cgtcccaggc ctgcggcacg aacttcttgc
     4381 cgagggcgat cacccggtcg cccaccgcgt agagcttcac ttcctccccg gtgtcctgga
     4441 agccgcggtc cagcagcagc gcgctctccg ccggggagtc gatcagcccg cgcaggaagc
     4501 gatcggggaa gagcacgtcg gcgtccatga tgagagtgct gtcgcgaagc agggtgtccc
     4561 gcgcacacag tagcgacagg atcgaaccct tctggtagtc gggattcacc acgtacccga
     4621 tccgcatcgc gccgcgagcg gggcccgccg cgcgacggac ctggtcctcg cagtggccga
     4681 gcaccaggac cgcctcgtcc acccccgcgg ccgagagggc gtcgagcatg cgatcgagca
     4741 gggagcggcc gcccaccggc agcaggcact tgtgggtgtg atcggtgagc ggcgcgaggc
     4801 ggcgcgccac ccccgcggcc aggatgaccg ccttcaccgg cgctcgcggc cgaaggaggc
     4861 gcggaaccgg tcgcggatct cctccggcgg gtacgggatg cgcggcgcgg gctgctcctc
     4921 ggcggtcacc cgcgccagga tgaagctcgg gccaccgccg cagagcgcgg tggtcaccgc
     4981 gtcacgaatc tcgtcggcgc ccgcgaccgc gacgctccgc tcgtagcccg cggcccgcgc
     5041 gatccggtgc aggccgaccg cgcgcgaggg cgaggcctgg ttgccggtcg agccgtagag
     5101 cgcgttgtcg aagaccacgt gcaccaggtt ggccggccgc tggcgtccca tcaccgggcc
     5161 cccgccgatc atgggcagga tcccgaggct catgagcagg ttgccgtcgc cgtcgaagac
     5221 cacggtggag cggtcgggca ccgcgagggc gactccgagc ccgatcgcgg aggccatccc
     5281 catcgacccg atcatgtaga agttctcggg gcggtccttg accccgaacg actcgcggca
     5341 gatgtagccg ttggcgtgca ccaccggctc cgcggtgagg ggagccaccg cggcgcgcag
     5401 cgcctcgaag cgcgagatgc gcggggccgg gatcgggtcg tcctggctcg tcgcccgcga
     5461 gaccggcgcg ccggcggggg cggacgccgc ggccgcgtgg gacgcggtgt cgaggatgcc
     5521 gggcggcagg agcagcgcca ccggctggct caggcgctcc gactccgcgc ggccccacgc
     5581 gacgtcgtcg gcgatggtct cggccgagag gatgcggtga gggatgcgca gcagctcgag
     5641 cagggcgggg gagatctcgc ccatcagcag gtgctcgggc gcgtccttgc cgcggtggcc
     5701 gcgccaggtc accagcagca gcgcgggcag ccggtacatc agggagagcg acaccagcgc
     5761 gttcatgctc gtgccgagcc ccgagttctg catcagcacc atcggccggc ggcccgcgag
     5821 ccacgcgccg gccgcgagcc ccaccgccac gtcctcgcgc accgcgggca cgtagggcag
     5881 gcgcgggtgg gtctccaggg tcgcgatcag gccctcgatc atcgagcagg gcaccccggt
     5941 gaagaagtcg tagccctggg cttcgagcag cccggcgagg gcggggccgg tcaggccttg
     6001 atcgccgccc atgcccgctg gtagtcctcg aacgtgtcga cctcgagcca gcccttgtag
     6061 acgtcgacgc acgccaccgt ctcgccccgc tgcaccagct cctgcagcag atcggtgaag
     6121 gcgccgcgct ccggccggtc cgcctcgtgg aagggcccgg tgcccgcggc catccgctgc
     6181 tggtaggcct cgcgcatcgc ggcggtgccc cgctcggaga agagggcgag cccgatgaac
     6241 tcgccg
//
LOCUS       DATMCV010000362         3819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_422010, whole genome shotgun sequence.
ACCESSION   DATMCV010000362 DATMCV010000000
VERSION     DATMCV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422010"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VNU03_08455"
     CDS             <1..202
                     /locus_tag="VNU03_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWP74533.1"
                     /translation="ELVGRILDCAPTAVRLQKELMVRWRNTDQRTAVEYGINAFAQSF
                     ATDEPREAMDAYLGKRKPRFAP"
     gene            199..954
                     /locus_tag="VNU03_08460"
     CDS             199..954
                     /locus_tag="VNU03_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP74534.1"
                     /translation="MIRLVTFDFWDTLVTDSPANLRAQRTLRVTAIRRALRDAGEMVS
                     EAEAEDIHERSGVLLAERFWSLNRDPSPEEQLRLVLDTVQPGVAARLAPPAFAAALEA
                     YTEPVLAYPPELCPNAAQAVRALAARGVAVGIVSNTGRTPGAVLRRVLERYGLLAYFG
                     AVSYSDEVGVRKPAAEIFRVTLARAGVAAGEALHIGDNPDADVVGAQGVGMRAAHYTA
                     GFRRASAQADLIIPDLSMLADEVFRVASPATGG"
     gene            967..1491
                     /locus_tag="VNU03_08465"
     CDS             967..1491
                     /locus_tag="VNU03_08465"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWP74535.1"
                     /translation="MDLDELRAKIRDIKDFPTEGILFKDITTLLRDGPAWAAVIDHLA
                     ARYHTARVDLVVGVESRGFIFGGALAHQLKAGFVPVRKRGKLPARTIEEEYELEYGRD
                     VLAIHEDAVTPGQRVLVVDDLLATGGTMAATLRLIARLGGTVVGAAFLIELAFLKGRA
                     RLAGQRIDSLIVYE"
     gene            1536..1880
                     /locus_tag="VNU03_08470"
     CDS             1536..1880
                     /locus_tag="VNU03_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74536.1"
                     /translation="MIDRALAGGLLAVVAGLGLPAVAGAAGETPSASSIILNLTNRPL
                     ESRERAFSEDIKRDALAPRPSAIDEWEPQPDGSMRNKKTGISVKLMNPCPPGDMEHEF
                     ALAAYNRSKSRR"
     gene            1961..2818
                     /locus_tag="VNU03_08475"
     CDS             1961..2818
                     /locus_tag="VNU03_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016423138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HWP74537.1"
                     /translation="MAIRNVGVLGCGLMGSGIAQVSAQAGFPTVVVEASQELLDRGLG
                     GLRRGLDALVAKAKLDERGRDEIMGRIKGVVGLEAFRDCDLVIEAITENQPLKNETFA
                     KLDRICPPHALLASNTSSCNITAMAAATKRPGQVLGLHFFNPVPLMKLVEVVQTILTD
                     EKSVLAAYEWVQAVAKVPVRAKDSTAFIVNRLLVPYLLDAIRVYEGGLASLEDIDQAM
                     KLGCGYPMGPFTLLDLVGLDTTMFVAEVMFDEYREPRYAPPPLLKRMVMAGQLGRKSG
                     KGFYDYSKK"
     gene            2834..3129
                     /locus_tag="VNU03_08480"
                     /pseudo
     CDS             2834..3129
                     /locus_tag="VNU03_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            3190..>3819
                     /locus_tag="VNU03_08485"
     CDS             3190..>3819
                     /locus_tag="VNU03_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP74538.1"
                     /translation="MTAAFSLSEPGSGSDAAAVTCRAVRKGDRYVLNGSKMWVTNGSH
                     AGVITVFVTTEPGKRAKGVTALLVEPGLKGFTVGKPERKLGIHGSPTVALHFGDCEVP
                     VENRLGAEGEGFKFAMKTLEQSRPTIGAQAVGIAQAGLDASVAYAKERRAFDQPIGQF
                     QGLQFMLADMAMAVHASRLVVHHAAGLIDHGITGTALEASVAKCLASDTA"
BASE COUNT          571 a         1326 c         1353 g          569 t
ORIGIN      
        1 ggagctggtc ggccgcatcc tcgactgcgc gcccaccgcg gtgcgcctgc agaaggagct
       61 gatggtccgg tggcgcaaca ccgaccagcg caccgcggtc gagtacggga tcaacgcctt
      121 cgcccagtcg ttcgcgaccg acgagccccg cgaggcgatg gacgcctacc tcggcaagcg
      181 caagccccgc ttcgctccgt gatccgtctc gtcacgttcg acttctggga caccctcgtc
      241 accgactcgc ccgccaacct gcgcgctcag cgcacgctcc gggtcaccgc catccggcgg
      301 gcgctccgcg acgcggggga gatggtgagc gaggccgagg cggaggacat ccacgagcgc
      361 tcgggggtgc tgctcgccga gcgcttctgg agcctcaacc gggacccgag tcccgaggag
      421 cagctgcgcc tcgtgctcga taccgtccag ccgggggtgg ccgcgcgcct cgccccgccc
      481 gcgttcgccg ccgcgctcga ggcctacacc gagccggtgc tggcctatcc cccggagctc
      541 tgcccgaacg cggcccaggc ggtgcgcgcg ctggccgcgc gcggcgttgc ggtcggcatc
      601 gtgtccaaca ccggccgcac gcccggggcg gtcctgcgcc gggtcctcga gcgctacggc
      661 ctgctcgcgt acttcggcgc ggtctcctat tccgacgagg tcggcgtccg gaagccggcg
      721 gccgagatct tccgcgtgac cctcgcgcgc gcgggcgtgg cggcgggcga ggcgcttcac
      781 atcggcgaca acccggatgc cgacgtggtc ggcgcgcagg gggtcggaat gcgcgcggcg
      841 cactacaccg cgggctttcg ccgcgcctcc gcccaggcgg acctgatcat cccggatctc
      901 tcgatgctgg cggacgaggt ttttcgggtt gcgtcacctg cgaccggcgg gtaacatccc
      961 agcgccatgg acctggatga gctgcgcgca aagatccgcg acatcaagga tttccccacc
     1021 gagggcatcc tcttcaagga catcaccacg ctgctgcgcg acgggccggc gtgggccgcg
     1081 gtcatcgacc acctggccgc gcgctatcac accgcccggg tggatctggt ggtgggcgtc
     1141 gaatcgcgtg gcttcatctt tggcggcgcc ctcgcgcacc agctcaaggc ggggttcgtg
     1201 ccggtgcgga agcgcggcaa gctcccggcc cgcacgatcg aggaggagta cgagctcgag
     1261 tacggccgcg acgtgctggc cattcacgag gacgcggtga caccgggcca gcgggtgctg
     1321 gtggtcgacg atctcctcgc gaccggaggc accatggccg ccacgctgcg actcatcgcc
     1381 cgcctggggg gcaccgtggt cggggccgcc ttcctgatcg agctggcgtt cctcaaggga
     1441 cgcgcccgcc tcgccggtca gcggatcgac tccctgatcg tctacgagta agcgcccgga
     1501 agtagttgta tgatgggggc cgggaggcaa ccgcaatgat cgatcgagcg ttggccggcg
     1561 gtctgctggc ggtggtggcg gggctcgggc tgcccgcggt ggcgggggcg gccggggaga
     1621 ctccctcggc gtcgtccatc atcctgaacc tgacgaaccg gcccctggag tcccgcgagc
     1681 gcgccttcag cgaggacatc aagcgggacg cgctggcgcc gaggccgtcc gccatcgacg
     1741 agtgggagcc ccagccggac ggttcgatgc gcaacaagaa gaccgggatc agtgtgaagc
     1801 tcatgaaccc gtgtccgccg ggcgacatgg aacacgagtt cgcgctggcc gcttacaacc
     1861 ggtcgaagtc ccgccgctga ccgtcgcgcc gatcccttcc attcgaggct gacacctgcc
     1921 cccgggcccc gggggcgacg ctgatctgga gacaccacac atggcaattc gcaacgtcgg
     1981 cgtgctgggc tgcgggctga tggggtcggg gatcgcccag gtctcggccc aggcgggctt
     2041 cccgacggtg gtggtggagg cgagccagga gctgctcgat cgcgggctcg gcggcctccg
     2101 ccggggcctg gacgcgctgg tggccaaggc caagctcgac gagcgcggcc gggacgagat
     2161 catgggccgc atcaagggcg tggtcgggct cgaggccttc cgcgattgtg acctggtgat
     2221 cgaggccatc accgagaacc agccgctcaa gaacgagacc ttcgccaagc tcgaccggat
     2281 ctgcccgccg cacgcgctgc tcgcctcgaa cacgtcgtcc tgcaacatca ccgcgatggc
     2341 cgccgccacc aagcggccgg gccaggtgct ggggcttcac ttcttcaacc cggtgcctct
     2401 gatgaagctg gtggaagtgg tgcagaccat tctcaccgac gagaagagcg tgctcgcggc
     2461 ctacgagtgg gtgcaggcgg tggccaaggt accggtgcgg gccaaggact cgaccgcgtt
     2521 catcgtgaac cggttgctgg tgccgtacct gctcgacgcc atccgggtct acgagggtgg
     2581 cctggccagc ctcgaggaca tcgaccaggc catgaagctc ggctgcggct atccgatggg
     2641 gcccttcacc ctcctcgacc tggtcgggct cgacacaacc atgttcgtcg ccgaggtgat
     2701 gttcgacgag taccgcgagc cccgctacgc gccgcccccg ctgctcaagc ggatggtgat
     2761 ggcgggccag ctgggccgga agagcggcaa gggcttctac gattactcga agaagtagga
     2821 ccgcgagacc ggcatgaaca atctcttcta tctcaccgac gagcagcgcg cggtccgcga
     2881 tctggcccgc gagatcgccc gcgagcgcat cgcgccgctg gccgcccacg tcgacgagac
     2941 tgggagtacc cccacgagca gctcaagcta ctgggccagc agggattgat gggcctccac
     3001 atcccggaag cctacggcgg gagcgcggcc ggctctctcg cgttctgctt ggccgccgag
     3061 gaggtggcgt gggcctgtgc ctcgacgtcc accatcttcc tggtgcagaa cctcgggggc
     3121 tacccgatca tcttcggggg cagcgaggag cagaagcggc gctacctgcc caagctggcc
     3181 tccggggaga tgaccgcggc gttctcgctc tccgagcccg gctcgggctc cgacgcggcg
     3241 gccgtgacgt gccgcgcggt gcgcaagggc gatcgctacg tgctgaacgg ctccaagatg
     3301 tgggtgacca acggctcgca cgcgggcgtg atcacggtgt tcgtcacgac cgagcccggc
     3361 aagcgggcca agggcgtcac cgcgctcctg gtggagcccg gcctgaaggg cttcacggtg
     3421 ggcaagcccg agcgcaagct cggtatccac ggctcgccca cggtggcgct ccacttcggc
     3481 gactgcgagg tgccggtgga gaaccggctc ggcgccgagg gcgaggggtt caagttcgcg
     3541 atgaagaccc tcgagcagtc gcgccccacc atcggggccc aggcggtggg catcgcccag
     3601 gccgggctcg acgcgtcggt ggcctacgcg aaggagcggc gggcgttcga tcagcccatc
     3661 ggccagttcc aggggctcca gttcatgctc gcggacatgg cgatggcggt ccacgcctcg
     3721 cgcctggtgg tgcaccacgc ggccggtctc atcgaccacg ggatcaccgg taccgcgctc
     3781 gaggcctcgg tggcgaagtg cctggcctcc gataccgcc
//
LOCUS       DATMCV010000363         2467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_885147, whole genome shotgun sequence.
ACCESSION   DATMCV010000363 DATMCV010000000
VERSION     DATMCV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885147"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1360
                     /locus_tag="VNU03_08490"
     CDS             68..1360
                     /locus_tag="VNU03_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HWP74539.1"
                     /translation="MTLREAARSVFDAGLAAADVAPLVRRALADVRLAPSGRVRVVGA
                     GKAPGAMAAAAEAALGDVIADGLVVVKDGYRAPTHRITLVEAGHPVPDARGEAAAHRI
                     RALAEACGADDLLLVLVSGGGSALTPAPVPPITLADKQAMTRLLLAAGANINQLNAVR
                     KHCSLLKGGQLARAAAPARVHALLLSDVIGDPLDVIASGPTAPDVSTFADARAILERF
                     DLLARAPAPIVDRLERGIRGEVPETPKPDDPVFRRVTNTVIGNNALVVDAASVRARAL
                     GYTPHVLTRSLEGEAREAADRLVVLARQFREGRGPVAAPACVIAGGETTVTVRGKGAG
                     GRCQEFGLAAALALEGQDGVVALAAGTDGTDGPTDAAGALVDGGSARRARERGLDPRA
                     SLDANDSNPVLAATGDLVVTGPTNTNLLDLYLLLIDPR"
     gene            complement(1357..2364)
                     /locus_tag="VNU03_08495"
     CDS             complement(1357..2364)
                     /locus_tag="VNU03_08495"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HWP74540.1"
                     /translation="MRIRPLLVALILLAAALPAAAADDAQLARGIQAVRRMAGCFLVD
                     YSYVEVQALKPGYVRDPRVYDVNRDKSAKEWIVAETISPRRIRLQRVLFLADLAGVLR
                     PGTEVRHQSEDWEFDAPFLYDFVSPLHWRPRDLRPTPGLWTRRVTNLDDGLRYQCAAR
                     WSEDTAYPEWACGSNYAPIPGRETRDMGRSDYQALERSTRIVVYGASQGGGASPGGSW
                     LERQDNVKTIHGDGGRVPLVRELGKNWYVRLPDAECAGGQAFAEAHRSIWTLLRETWD
                     GVLDGSGPFVERAPAGQPPRFVKMFELEDSVLDRDLSDQAIRQEIRRRILDVIQEYRA
                     P"
BASE COUNT          313 a          920 c          926 g          308 t
ORIGIN      
        1 gcttctgttt ccccaagacc gacgacatgc tgatcgaggc ggggcgccgg ctgcagaagc
       61 tcgcccgttg accctccgcg aggcggcgcg ctcggtgttc gacgccgggc tcgcggccgc
      121 cgacgtcgct ccgctggtcc gccgcgcgct cgcggacgtg cgcctcgccc cgtccggccg
      181 cgtgcgggtg gtgggcgcgg ggaaggcccc gggggcgatg gccgcggcgg ccgaggccgc
      241 gctcggcgac gtcatcgcgg acgggctggt cgtggtcaag gacggctacc gcgcgcccac
      301 ccaccgcatc acgctggtcg aggccggcca cccggtgccg gacgcgcgcg gcgaggcggc
      361 cgcgcaccgg atccgggcgc tcgcggaggc gtgcggggcg gacgacctgc tgctggtcct
      421 ggtctcgggc gggggctcgg ccctcacccc ggcgccggtg ccacccatca cgctggcgga
      481 caagcaggcc atgacgcggc tgctcctcgc cgccggcgcc aatatcaacc agctgaacgc
      541 ggtgcggaag cactgctcgc tgctcaaggg cggccagctc gcgcgcgcgg ccgcgcccgc
      601 ccgcgtgcac gcgctcttgc tctcggacgt gatcggcgat ccgctcgacg tcatcgcctc
      661 cggccccacc gcgcccgacg tctccacctt cgcggacgcg cgggcgatcc tcgagcgctt
      721 cgacctgctg gcccgcgcgc ccgccccgat cgtggaccgg ctcgagcgcg ggatccgggg
      781 cgaggtcccg gagacgccga agcccgacga cccggtcttc cgtcgcgtca ccaacaccgt
      841 catcggcaac aacgcgctgg tcgtcgacgc ggcgtccgtg cgggcgcgcg ccctcggcta
      901 caccccgcac gtgctgaccc gctcgctcga gggcgaggcg cgcgaggcgg ccgaccggct
      961 ggtcgtgctg gcgcggcagt tccgcgaggg gcgggggccg gtggccgcgc ccgcctgcgt
     1021 gatcgcgggc ggcgagacca ccgtgacggt gcggggcaag ggagccggcg ggcgctgcca
     1081 ggagttcggc ctggccgccg ctctcgcgct cgagggtcag gatggcgtgg tggcgctggc
     1141 cgcgggcacc gacggcaccg acgggccgac cgacgcggcg ggagcgctgg tggacggcgg
     1201 cagcgcccgg cgggcgcgcg agcggggcct cgatccacgg gcgagcctcg acgcgaacga
     1261 ctcgaacccg gtgctggcgg ccaccggtga cctggtcgtc acgggcccca ccaacacgaa
     1321 cctcctcgac ctctacctac tcctgatcga cccgcgctaa ggtgcgcggt attcctgaat
     1381 cacgtccaag atgcgccggc ggatctcctg ccggatcgcc tgatccgaga gatcgcggtc
     1441 gagcaccgag tcctcgagct cgaacatctt gacgaagcgc ggcggctgcc cggccggcgc
     1501 acgctccacg aagggcccgg agccgtccag gaccccgtcc cacgtctcgc gcagcagggt
     1561 ccagatcgag cggtgggcct cggcaaaggc ctgcccgcct gcgcactccg cgtccggcag
     1621 ccgcacgtac cagttcttgc cgagctcgcg caccagcggg acccggccac cgtcgccgtg
     1681 aatggtcttc acgttgtcct ggcgctcgag ccagctgccg cccggcgagg cccccccgcc
     1741 ctgcgaggcc ccgtagacca cgatgcgggt cgatcgctcg agggcttgat agtccgagcg
     1801 gcccatgtcg cgggtctcgc gccctgggat cggcgcgtag ttgctcccgc acgcccactc
     1861 ggggtacgcg gtgtcctcgc tccagcgcgc cgcgcactgg tagcgcagcc catcgtcgag
     1921 attggtcacg cggcgggtcc agaggccggg cgtcgggcgc aggtcgcgcg gccgccagtg
     1981 cagcggcgac acgaagtcgt agaggaacgg ggcgtcgaac tcccagtcct ccgactggtg
     2041 ccgcacctcg gtgccgggcc ggaggacgcc ggccaggtcg gccaggaaca ggacgcgctg
     2101 cagccggatc cgccggggcg agatcgtctc ggcgacgatc cactccttcg ccgacttgtc
     2161 gcggttgacg tcgtagacgc gcgggtcgcg cacgtagccc ggcttcagcg cctgcacctc
     2221 gacgtagctg tagtcgacca ggaagcagcc cgccatgcgc cgcaccgcct ggatgccgcg
     2281 ggcgagctgg gcgtcgtcgg ccgcggcggc aggcagggcg gcggcgagca ggatcagcgc
     2341 gacgaggagc gggcggatgc gcatcgagag gcctcgcgcc ctagggtcgc ccggggcgcg
     2401 aggcctgtca actgcgcggc gagccggcgc ggctagaagc gcacgcggac ccggtaggcg
     2461 agggtgg
//
LOCUS       DATMCV010000364         3204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_87695, whole genome shotgun sequence.
ACCESSION   DATMCV010000364 DATMCV010000000
VERSION     DATMCV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87695"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VNU03_08500"
     CDS             complement(<1..218)
                     /locus_tag="VNU03_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74541.1"
                     /translation="MTRHRADRRAFVATVATGMLLMGRPAAAQSPPPVPAPGAPRVWR
                     IGFLTPSAVSPRGIMPDMRAKLRELGYV"
     gene            290..667
                     /locus_tag="VNU03_08505"
     CDS             290..667
                     /locus_tag="VNU03_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP74542.1"
                     /translation="MGRARILVIDDERAVRELISDALKIEGHDVHTAENGKEGLDLIG
                     RNRFDLVFCDLRMPEMDGQQLYEEVQRDHPQVLKRIVFVTAQANSSDYGPFLRTTGIP
                     VIEKPFTLSQLRQMVGKMVGVMR"
     gene            complement(664..1563)
                     /gene="rho"
                     /locus_tag="VNU03_08510"
     CDS             complement(664..1563)
                     /gene="rho"
                     /locus_tag="VNU03_08510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWP74543.1"
                     /translation="MPPRPPSRPQVPSLFEGLTAVQPYEKLKLETTADQLSMRALDMI
                     APIGRGQRGLIVAPPRTGKTILLQQIAKAVLANHPEVAVIVLLVDERPEEVTDFKMQI
                     GKQSEIVASSNDNPYRRHIEVTEQALDRAKRMVEEKRDVLILFDSLTRMTRAYNNELS
                     SRGRTMSGGIDSRAFQMPRAFFGAARKLEEGGSLTIIGTVLVDTGSRMDQIIFEEFKG
                     TGNMELYLSRELADRRIFPSFDLMRSGTRREELLFSEEEMKKIHLLRRALSGTKPQEA
                     MEAMLGRLKLTATNKDFLKGLGA"
     gene            1866..3017
                     /locus_tag="VNU03_08515"
     CDS             1866..3017
                     /locus_tag="VNU03_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWP74544.1"
                     /translation="MTPRADVVGSLLRPEALRLARQGAREGRVTPEALRAAEDRAVRE
                     AIALQEGAGLDVITDGELRRSSWVITIPLREDAAGRPPLGGFEFLPADPGWWSLWKEP
                     DGRRAQVWTSPTRPFITRPIQVARDVVSDEYAFLRANARARSKFTIPSPSWHRIFWHP
                     EYSRAAYPTPEDFLRAIAGYLREQVVPRIIALGGDYIQMDAPNYAQWHVDGENRAAFE
                     AWGHDMAAELVADAEIDDTVFEGVRGVTRAIHICRGNAPGGRWLANGGYERIAAEVFP
                     RLTRYDRLLLEYDTPRAGDFGPLRHVRPEATVVLGLLTTKQGELEDAALVETRIREAA
                     RQVPLERLALSPQCGFASGEAGNPLTPAQQEAKLRRVATVARRVWPGQG"
BASE COUNT          470 a         1103 c         1172 g          459 t
ORIGIN      
        1 tccacgtagc ccagctcgcg cagcttcgcg cgcatgtcgg gcatgatccc gcgcggcgac
       61 accgcggagg gggtgaggaa gccgatccgc cagacccggg gcgcgccggg ggccggcacg
      121 ggcggcgggg actgggcggc ggcgggccgt cccatcagca gcatgccggt cgccacggtg
      181 gcgacgaagg cgcggcggtc ggcccggtgc ctggtcattg gagttctcac cctcccccgt
      241 cgtagaatag ctggaaccgc gaccacgtca acatctgacg gggaccaaca tgggacgagc
      301 ccgaatcctg gtgatcgacg acgagcgtgc cgtccgggag ctgatctcgg acgcgctgaa
      361 gatcgagggg cacgacgtcc acaccgcgga gaacggcaag gaaggcctcg acctcatcgg
      421 gcgcaaccgc ttcgacctcg tgttctgcga cctccgcatg cccgagatgg acgggcagca
      481 gctctacgag gaggtccagc gggaccatcc ccaggtgctc aagcggatcg tgttcgtgac
      541 cgcccaggcc aactcgtcgg actacggccc cttcctgcgc accaccggga tcccggtcat
      601 cgagaagccc ttcaccctga gccagcttcg ccagatggtc ggcaagatgg tcggggtgat
      661 gcgctaggcg cccagcccct tcaggaagtc cttgttggtg gcggtgagct tgagccgtcc
      721 cagcatcgcc tccatcgcct cctgcggctt ggtgccggac agggcccggc gcagcaggtg
      781 gatcttcttc atctcctcct cggagaagag cagctcctcc cggcgggtgc ccgaccgcat
      841 caggtcgaac gaggggaaga tgcgccggtc ggccagctcg cgcgagaggt agagctccat
      901 gttgccggtg cccttgaact cctcgaagat gatctggtcc atccgcgagc cggtgtccac
      961 caggacggtg ccgatgatgg tgagcgagcc gccctcctcg agcttgcggg cggccccgaa
     1021 gaaggcgcgc ggcatctgga aggcccggct gtcgatgccg cccgacatgg tgcgcccccg
     1081 cgaggacagc tcgttgttgt aggcgcgggt catgcgggtg agggagtcga agaggatcag
     1141 cacgtcgcgc ttctcctcga ccatccgctt ggcccggtcg agggcctgct cggtcacctc
     1201 gatgtggcgg cggtaggggt tgtcgttgct cgacgccacg atctccgact gcttgccgat
     1261 ctgcatcttg aagtcggtga cctcctcggg ccgctcgtcc accagcagca cgatcaccgc
     1321 gacctcgggg tggttggcga ggaccgcctt ggcgatctgc tgcagcagga tggtcttgcc
     1381 ggtccgcggc ggggccacga tgagcccgcg ctggccgcgg ccgatgggcg cgatcatgtc
     1441 gagcgcccgc atggacagct gatccgcggt ggtctcgagc ttcagcttct cgtacggctg
     1501 gaccgcggtg aggccttcga agagggaggg aacctgaggt cgggaagggg ggcgcggggg
     1561 catcggcccg cgtggccctt gcccttgtcc ttggccctgg ccttgccctt ggccctgtcc
     1621 ctgcccctgc ggcggtcgat taccggaatg ccggcggcgg tgagaccgcc tactcaacgg
     1681 acacgctcat ggcgcacgaa ccctttcggt acagttagag gacaatcgcc acgcccatca
     1741 accagcggtg gagggggcgg cggcgggggg acggccacac cacccgggtg cccacatggt
     1801 acaacggctg cgcccactaa agtaagcacc ccgtgtggca tgatggggcc cccgagggac
     1861 cgccgatgac accccgagcc gacgtggtgg gcagcctgct ccgtccggag gccctgcgcc
     1921 tggcgcggca gggcgcccgc gagggacggg tgacgcccga ggccctgcgg gcggccgagg
     1981 accgggcggt ccgcgaggcc atcgcgctcc aggagggcgc ggggctcgat gtgatcaccg
     2041 acggcgagct ccggcgctcg tcctgggtca tcacgatccc gctccgggag gacgcggccg
     2101 gccggccgcc gcttgggggc ttcgagttcc tccccgccga cccgggctgg tggtcgctgt
     2161 ggaaggagcc ggacggccgt cgggcccagg tgtggacgag ccccacccgt cccttcatca
     2221 cccggccgat ccaggtcgcg cgcgacgtgg tcagcgacga gtacgcgttc ctcagggcca
     2281 atgcgcgggc ccggtccaag ttcacgatcc cctccccgag ctggcaccgc atcttctggc
     2341 accccgagta ctcgcgcgcg gcctacccga ccccggagga cttcctgcgc gcgatcgcgg
     2401 gctacctgcg cgagcaggtg gtgccgcgga tcatcgcgct gggcggcgac tacatccaga
     2461 tggacgcgcc caactacgcc cagtggcacg tggacgggga gaaccgcgcc gccttcgagg
     2521 cctggggcca cgacatggcg gcggagctgg tcgccgacgc ggagatcgac gacaccgtgt
     2581 tcgagggtgt gcgcggagtc acccgcgcca tccacatctg ccggggcaac gcgcccgggg
     2641 gacgctggct cgccaacggg ggctacgagc ggatcgccgc ggaggtcttc ccacgcctca
     2701 cccggtacga tcgtctcctc ctcgagtacg acaccccgcg cgcgggcgac ttcgggccgc
     2761 tccgtcacgt gcggccggag gccaccgtgg tgctcgggct gctgaccacc aagcagggtg
     2821 agctcgagga cgcggcgctc gtggagacgc gcatccgcga ggcggcgcgt caggtcccgc
     2881 tggagcgcct ggccctgagc ccgcagtgcg gcttcgcctc gggcgaggcg ggcaacccgc
     2941 tcacgcccgc gcagcaggag gcgaagctcc ggcgggtggc gaccgtagcc cggcgggtct
     3001 ggccgggcca agggtagcgc caggtgagga caggcgcccg gcgggtctgg ccgggctaag
     3061 ggtagcgcca ggtgaggaca ggcgcgcggc gggtctggcc cgcgggctag cgccgcgcgc
     3121 tcggcgcgta gaacgggtcg cgcggggcct cgtcgagcca gccgtcgtag aaccgccgca
     3181 cgtgcgggaa gacctcgcgg gcga
//
LOCUS       DATMCV010000365         6766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_87779, whole genome shotgun sequence.
ACCESSION   DATMCV010000365 DATMCV010000000
VERSION     DATMCV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87779"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="VNU03_08520"
     CDS             complement(<1..1678)
                     /locus_tag="VNU03_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007826045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWP74545.1"
                     /translation="MAMSAEQIRLLDAYWRAANYLTVGQIYLRDNPLLREPLTPAHVK
                     PRLLGHWGTSPGLSFIYAHLNRLVRETDADVIYLAGPGHGGPAIVANVYLEGTYSEIY
                     PDVSQDAAGLARLFRQFSTPGGIPSHVSVTTPGSIHEGGELGYVLVHAFGAAFDNPEL
                     IVAAVVGDGEAETGPLAGSWTGIDFLSPARDGAVLPILHLNGYKIGGPTVLGRLDDDD
                     IVSLLRGHGWDPHLVEGEDPLVMHPRFAQVLDDCHARIRAIQAEARRPGVDPARLARP
                     RWPAIVLRTPKGWTGPRMVDGKPVEGTFRAHQVPVDRVREDPAHLALLDGWMRSYRPE
                     ELFDDRGRLRAELAALAPSGDRRLGANPHANGGRVLRPLDLPDYADYALPVPRPATEL
                     HGSTRQLGKLMRDIFTRNRAEANFRLFCPDETNSNRLGDVFDVENRCSVLPRKADDDH
                     VAPDGRVMEILSEHCCQGWLEGYLLTGRHGLFASYEAFAMVSASMTVQHAKWLQEADH
                     LPWRAPVASLNILLTSTSWRQDHNGFSHQGPGLIDVVLSKKGTVSRIYLPP"
     gene            complement(1683..2396)
                     /locus_tag="VNU03_08525"
     CDS             complement(1683..2396)
                     /locus_tag="VNU03_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP74546.1"
                     /translation="MSSRPAAVAFLVDVDNTLLDNDRVTEDLRHYLDRTVGPEGRARY
                     WAHFERLRVELGYADYLGALQRYRLEFPRDPHLLTVSAFMIDYPFANRLYPGSLDVLE
                     RFRGWGPTVIMSDGDVVFQPRKIQRSGLGDAVDGVLIYVHKERELDDVEQRYPADHYV
                     LVDDKLRVLAAVKGAWGARVTTVFPRQGHYAHDPEILATNPAADLTVERIGDLVDFDL
                     STLRARASAGVAAGPGSGR"
     gene            complement(2393..3406)
                     /gene="gnd"
                     /locus_tag="VNU03_08530"
     CDS             complement(2393..3406)
                     /gene="gnd"
                     /locus_tag="VNU03_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWP74547.1"
                     /translation="MQLGMIGLGRMGANMVRRVQKAGHECVVYDVHADAVSALAREGA
                     SGAASLPAFVKALARPRTVWLMVPAGVVDRTLEDLVPLLEPDDVIIDGGNSHYHDDIR
                     RAGELRARRLHYVDVGTSGGVWGLERGFCLMIGGEAPVVQRLDPIFEALAPGIGAAPR
                     TGGREQIGGTAEHGYLHCGPSGAGHFVKMVHNGIEYGLMAAYAEGLNILHHANAGASQ
                     RTTDAETSPLRNPEHYRYDLDLRDITEVWRRGSVVASWLLDLTASALTKSHDLASFSG
                     RVSDSGEGRWTVQAAIDESVPAPVLSTALYERFSSRGEGDFAGKVLSALRFEFGGHHE
                     KPA"
     gene            complement(3391..4743)
                     /locus_tag="VNU03_08535"
     CDS             complement(3391..4743)
                     /locus_tag="VNU03_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74548.1"
                     /translation="MTPMSASAEGLLAHAVASFEAGVPTLQAIAKDFQAQIAQGLSGV
                     GGSLKMLRTFTHQPSGRERGRVVVVDWGGTKARAGLVELAGGGSARIVTEEALTFPEA
                     MKRGAPEPVFDLIAGAVGRVARAQGVDGAPLAFVYSYPARLERIDRAVALASTKGWRL
                     QGLVGRDVAAMLTAALERAELARIAVAAVANDTVAALILQSYQARSRDAQARPADVGL
                     ILGTGTNLAADLGPAGIRNLESGNFDGVGAVGTPYDVALDRELDEPPPGAQRFEKMVS
                     GHYLGEILRRALVDLGTRTGWFRWSSAPVIATPFALDSEYLSRIAADRTAALDDVDAL
                     LRSLGIESTREERHGVGALGAAIARRAGRLVAAAFVGTLRFVDPRLDERRTIAVDGSL
                     WGGYPGIEGLARGALAELLGRERADRVEIAFVKDSTSAGAAVIAAVAAAQRSEGCSSA
                     "
     gene            4785..5993
                     /gene="ispG"
                     /locus_tag="VNU03_08540"
                     /gene_synonym="gcpE"
     CDS             4785..5993
                     /gene="ispG"
                     /locus_tag="VNU03_08540"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWP74549.1"
                     /translation="MSTTERRKTVAVDVGGVKVGGKRPIVVQSMTNTDTADVAGTVAQ
                     VNALHAAGSELVRVTVNTEAAARAVPEIVKQVAVPVIGDFHYNGHVLLTKYPDCARAL
                     AKYRINPGNVGGKHHDENFSAIVRVAVDNGKPVRIGVNWGSLDQNLLTQMMDANAKSK
                     DPLTARDVTMNAMVESAIQSAELAEQTGLGHDRIILSAKVSGVQDLVDVYRKLAPRSD
                     YPLHLGLTEAGMGAKGIVASTAGLAILLQEGIGDTIRVSLTPKPNGDRTEEVQVAQLI
                     LQSLGLRSFLPLVTACPGCGRTTSTFFQEMAEEIQAYLRDQMPVWKGRYTGVEELKVA
                     VMGCVVNGPGESKHADIGISLPGTFEEPKAPVFVDGALKLTLKGDHIVADFLKILDDY
                     VEKRYAGSRT"
     gene            5998..6474
                     /locus_tag="VNU03_08545"
     CDS             5998..6474
                     /locus_tag="VNU03_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWP74550.1"
                     /translation="MGISDYLHDLRAKIGHDLLLTPGVAAVILNDAGHVLLQRRSDDG
                     EWGLPGGAMEPGEEPAESLVREVREETALEVVPERIVGVYSGPDFHVRYGNGDEAMIV
                     SITFACRSLGGEPRINDDESLELRYFAPDALPAMESRHRMRIEDALRGDPRARFRG"
     gene            complement(6484..>6766)
                     /locus_tag="VNU03_08550"
     CDS             complement(6484..>6766)
                     /locus_tag="VNU03_08550"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWP74551.1"
                     /translation="KQLQKALAEFDKVGERGAAQAAVLRFEIEFCERYLPKTMDEAGV
                     RALVKERIAALGIADPKQAGRLVGDIMKTHKGQVEAGDVKRVADELLKG"
BASE COUNT          990 a         2424 c         2423 g          929 t
ORIGIN      
        1 cgggcggcaa gtagatccgc gacaccgtcc ccttcttcga gagcaccacg tcgatcagcc
       61 cggggccctg gtggctgaag ccgttgtggt cctgacgcca ggaggtcgac gtgagcagga
      121 tgttgagcga ggcgaccggt gcccgccacg gcaggtggtc cgcctcctgc agccacttgg
      181 cgtgctgcac cgtcatcgac gccgagacca tcgcgaacgc ctcgtagctc gcgaagagcc
      241 cgtggcgacc ggtgagcagg tagccctcga gccagccctg gcagcagtgc tcgctcagga
      301 tctccatcac ccggccgtcg ggggccacgt ggtcgtcgtc ggccttgcgc gggagtaccg
      361 agcagcggtt ctccacgtcg aagacgtcac cgagccggtt ggagttggtc tcgtccgggc
      421 agaagagccg gaagttggcc tcggcgcgat tgcgcgtgaa gatgtcgcgc atgagcttcc
      481 cgagctggcg ggtcgagccg tgcagctcgg tggccgggcg cggaaccggc agcgcgtagt
      541 cggcgtagtc gggcaggtcg agcggccgga gcacgcggcc accgttggcg tgcggattcg
      601 cgccgagccg gcggtcgccg ctgggcgcga gcgcggccag ctcggcgcgg agccggcccc
      661 ggtcgtcgaa cagctcctcc ggtcgatagc tccgcatcca gccgtcgagc agcgccaggt
      721 gcgcgggatc ttcccgcacg cggtccaccg gcacctggtg cgcgcggaag gtgccctcga
      781 ccggcttgcc gtccaccatc ctgggcccgg tccagccctt gggcgtgcgg agcacgatcg
      841 cgggccagcg cggtcgcgca aggcgcgcag ggtcgacgcc gggacgtcgg gcctccgcct
      901 ggatggcccg gatccgggcg tggcaatcgt cgagcacctg ggcgaagcgc gggtgcatca
      961 ccagcgggtc ctcgccttcc acgagatggg ggtcccagcc gtggccacgc agcagactca
     1021 cgatgtcgtc gtcgtccagg cggccgagca cggtcggccc accgatcttg tagccgttca
     1081 ggtggaggat cggcagcacc gccccgtcac gggccggact caggaagtcg atgccggtcc
     1141 agctcccggc gagggggccg gtctccgcct ccccgtcgcc caccaccgcg gccacgatca
     1201 gctccgggtt gtcgaacgcg gcgccgaagg cgtggacgag cacgtagccc agctcgccgc
     1261 cctcgtggat cgagccggga gtcgtcacgc tcacgtggct cgggatgccg ccgggcgtcg
     1321 agaactggcg gaagagccgg gccagcccgg ccgcgtcctg cgacacgtcg gggtagatct
     1381 cggagtaggt gccctcgagg tacacgttgg ccacgatcgc gggcccgccg tgcccgggcc
     1441 cggccaggta gatcacgtcc gcgtcggtct cgcgcaccag acggttgagg tgcgcgtaga
     1501 tgaacgacag gcccggtgag gtgccccagt ggccgagcag ccggggcttg acgtgcgccg
     1561 gcgtgagcgg ctcccgcagc agcgggttgt cgcggagata gatctggccg acggtcaggt
     1621 agttcgcggc ccgccagtac gcgtcgagga gtcggatctg ctcggccgac atcgccatgc
     1681 gctcatctcc cgctaccagg tcccgcggcg acgccggcgg aggcgcgcgc ccgcagcgtg
     1741 gacaggtcga agtcgacgag gtcgccgatg cgctcgacgg tgagatcggc ggccggattg
     1801 gtggcgagga tctccggatc gtgggcgtag tggccctgcc gggggaacac ggtggtcacc
     1861 cgggcacccc acgcgccctt cacggcggcg aggacgcgga gcttgtcgtc caccaggacg
     1921 tagtgatcgg cggggtatcg ctgctcgacg tcgtcgagct cccgctcctt gtgcacgtag
     1981 atcaggaccc catcgaccgc gtcgcccagg ccggagcgct ggatcttgcg cggctggaac
     2041 accacgtcgc cgtccgacat gatcacggtc gggccccacc cgcggaagcg ctcgagcacg
     2101 tcgagcgagc ccgggtagag acggttcgcg aacgggtagt cgatcatgaa cgcggacacc
     2161 gtgagcagat gcgggtcgcg ggggaactcc aggcgatagc gctgcagcgc gcccaggtag
     2221 tccgcgtacc ccagctcgac gcggagccgc tcgaagtggg cccagtagcg cgcccggccc
     2281 tcgggcccca ccgtccggtc gaggtagtgc cggagatcct cggtgacgcg gtcgttgtcg
     2341 agcagggtgt tgtccacgtc gaccaggaac gcgaccgcgg cgggcctcga gctcacgcgg
     2401 gcttctcgtg gtggccgccg aactcgaagc gcagggccga gagcaccttg cccgcgaagt
     2461 cgccctcccc gcgcgagctg aagcgctcgt agagcgcggt gctcagcacc ggggcgggaa
     2521 cgctctcgtc gatggcggct tgcaccgtcc agcggccctc gcccgagtcc gacacccggc
     2581 ccgagaagct cgcgaggtcg tggctcttgg tgagcgcgct cgcggtgagg tcgagcagcc
     2641 acgaggcgac cacgctgccg cgccgccaca cctcggtgat gtcgcgcagg tccaggtcgt
     2701 agcgatagtg ctccgggttc cggagcggcg aggtctccgc gtcggtggtg cgctggctcg
     2761 cccccgcgtt ggcgtggtgc aggatgttga gcccttcggc gtacgcggcc atcaggccgt
     2821 actcgatgcc gttgtgcacc atcttcacga agtgcccggc ccccgacggc ccgcagtgca
     2881 ggtagccgtg ctcggcggtg ccgccgatct gctcgcggcc cccggtgcgc ggagcggcgc
     2941 cgatgccggg ggccagcgcc tcgaagatcg ggtcgaggcg ctgcaccacc ggggcctcgc
     3001 cgccgatcat gaggcagaac ccgcgctcga gcccccacac cccgccgctc gtccccacgt
     3061 cgacgtagtg gagccggcgg gcccgcagct cgcccgcccg ccggatgtcg tcgtgatagt
     3121 gggagttgcc cccgtcgatg atcacgtcgt ccggctcgag cagcgggacg agatcctcga
     3181 gggtccggtc caccaccccg gccgggacca tgagccacac cgtgcgcggg cgggcgagcg
     3241 ccttcacgaa ggccgggagc gacgcggccc cggaggcacc ctcgcgcgcg agggccgaca
     3301 ccgcgtcggc gtgcacgtcg tagaccacgc actcgtgccc cgccttctgc acccgccgca
     3361 ccatgttggc gcccatgcgc cccagtccga tcatgccgag ctgcatccct cgctcctttg
     3421 cgcggcggcc acggccgcga tgacggccgc ccccgcgctg gtcgaatcct tcacgaacgc
     3481 gatctcgacg cggtcggcgc gctcccggcc gagcagctcg gccagggccc cgcgcgcgag
     3541 tccctcgatg cccgggtagc cgccccagag acttccgtcc actgcgatgg tacgccgttc
     3601 gtcgagccgc ggatcgacga agcgcagcgt gcccacgaac gcggccgcca ccagccggcc
     3661 cgcgcggcgg gcgatggccg cgccgagtgc gccgacgccg tggcgctcct cccgcgtgct
     3721 ctcgatgccg agcgagcgca gcagcgcatc gacgtcgtcg agcgcggcgg tgcgatcggc
     3781 cgcgatgcgc gagagatact cgctgtcgag agcgaacggg gtggcgatca ccggcgccga
     3841 cgaccaccga aaccatccgg tccgggtccc caggtccacg agcgcgcgcc gcaggatctc
     3901 gccgaggtag tggccggaga ccatcttctc gaagcgctgc gccccgggcg gcggctcgtc
     3961 cagctcccgg tcgagggcga cgtcataggg ggttccgact gcgcccaccc cgtcgaagtt
     4021 ccccgactcc aggttgcgga tgccggccgg gcccaggtcc gcggccaggt tggtcccggt
     4081 gcccaggatg aggccgacgt cggccgggcg cgcctgcgcg tcgcggctcc gcgcctggta
     4141 gctctggagg atgagcgccg ccaccgtgtc gttggccacc gccgcgaccg cgatccgcgc
     4201 gagctccgcg cgctcgagcg cggcggtgag catggccgcc acgtcccggc cgacgaggcc
     4261 ctggagccgc cagcccttgg tcgaggccag cgccaccgct cgatcgatgc gctcgagccg
     4321 tgccgggtag gagtagacga aggcgagcgg ggcgccgtcg acgccctggg cccgggccac
     4381 gcggccgact gcgcccgcga tgaggtcgaa gaccggctcc ggcgccccgc gcttcatcgc
     4441 ctccgggaag gtcagcgcct cctcggtcac gatgcgggcc gaccccccgc cggccagctc
     4501 gaccaggccc gctcgcgcct tggtgccccc ccagtccacc accacgaccc gcccccgctc
     4561 ccggccggag ggctggtgcg taaacgtgcg cagcatcttg agcgagccac ccacgccgga
     4621 caggccctgg gcgatctggg cctgaaaatc cttcgcgatg gcctgaagcg taggcacccc
     4681 cgcctcgaac gaggccacgg cgtgcgccag gaggccctcg gcggaggcgg acatgggcgt
     4741 catgatactc cccggggtgg ggtggtatta ttggcatcgc gccaatgagc actaccgagc
     4801 ggcggaagac ggtggcggtg gacgtgggtg gggtgaaggt gggcggcaag cggcccatcg
     4861 tggtgcagtc catgaccaac accgacaccg cggacgtggc cggcacggtg gcccaggtca
     4921 acgcgctcca cgcggcgggc agcgagctgg tgcgcgtgac cgtgaacacc gaggcggcgg
     4981 cccgcgcggt gcccgagatc gtcaagcagg tcgccgtccc ggtgatcggc gacttccact
     5041 acaacggcca cgtgctgctg accaagtacc ccgactgcgc gcgggcgctc gccaagtacc
     5101 gcatcaaccc gggcaacgtg ggcggcaagc accacgacga gaacttcagc gcgatcgtcc
     5161 gggtcgcggt cgacaacggg aagccggtgc gcatcggcgt caactggggc agcctcgacc
     5221 agaacctgct cacccagatg atggacgcca acgccaagag caaggatccg ctcaccgcgc
     5281 gggacgtgac gatgaacgcg atggtggaga gcgcgatcca gtccgccgag ctggccgagc
     5341 agaccggcct cgggcacgac cggatcatcc tctcggccaa ggtctcgggc gtgcaggacc
     5401 tggtcgacgt gtaccgcaag ctcgcgcccc gctcggacta tccgctgcac ctcggcctca
     5461 ccgaggcggg catgggcgcc aagggcatcg tggcgagcac cgccggcctg gcgatcctgt
     5521 tgcaggaggg catcggcgac accatccgcg tctcgctgac cccgaagccc aacggcgatc
     5581 gcaccgagga ggtgcaggtc gcccagctga tcctccagtc cctcgggctc cggagcttcc
     5641 tgccgctggt gaccgcgtgc ccgggctgcg ggcgcaccac ctcgaccttc ttccaggaga
     5701 tggccgagga gatccaggct tacctgcgcg accagatgcc ggtgtggaag ggtcgctaca
     5761 cgggagtgga ggagctgaag gtcgcggtga tgggctgcgt ggtgaacggg cccggcgagt
     5821 ccaagcacgc cgacatcggc atctcgctgc ctggcacctt cgaggagccc aaggccccgg
     5881 tcttcgtgga cggcgcgctc aagctcaccc tcaagggcga ccacatcgtg gccgacttcc
     5941 tgaagatcct cgacgactac gtcgagaagc ggtacgccgg aagccgcacc tagcggcttg
     6001 ggcatctccg actacctcca cgacctccgg gccaagatcg gccacgacct cctgctgacc
     6061 ccgggggtcg ccgcggtcat cctgaacgac gcgggccacg tgctgctcca gcgccggagc
     6121 gacgacggcg agtgggggct ccccggcggc gccatggagc cgggcgagga gcccgcggaa
     6181 agcctggtgc gtgaagtgcg ggaggagacc gcgctcgagg tcgtgccgga gcgcatcgtg
     6241 ggcgtctaca gcggcccgga cttccacgtc cgctacggta acggggacga ggcgatgatc
     6301 gtcagcatca ccttcgcatg ccgctcgctg ggcggcgagc cgcggatcaa cgacgacgag
     6361 tcgctcgagc tgcgctactt cgcgcccgac gccctgcccg cgatggagtc gcggcatcgg
     6421 atgcggatcg aggacgccct tcgcggcgat ccgcgcgccc gcttccgcgg ctgagcggag
     6481 cgctcagccc ttcagcagct cgtcggcgac gcgcttgacg tcgcccgcct ccacctggcc
     6541 cttgtgggtc ttcatgatgt cgccgaccag gcggccggcc tgcttgggat cggcgatgcc
     6601 gagggccgcg atgcgctcct tcaccagcgc ccggaccccc gcctcgtcca tggtcttcgg
     6661 caagtagcgc tcgcagaact cgatctcgaa gcggagcacc gcggcctggg ccgccccacg
     6721 ctcgcccacc ttgtcgaact cggcgagcgc cttctgcagc tgcttg
//
LOCUS       DATMCV010000366         7417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_87835, whole genome shotgun sequence.
ACCESSION   DATMCV010000366 DATMCV010000000
VERSION     DATMCV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87835"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VNU03_08555"
     CDS             <1..407
                     /locus_tag="VNU03_08555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWP74552.1"
                     /translation="RPMLLSLPLRWPVRGPLNSRFGMRQSPWTGEQEFHRGLDISANR
                     GTAVLAPASGTVYFAGSSGEYGTTVILDHGHELRSLYGHLQEIRVKPGDRIDRGQVIA
                     LTGSTGRTSGPHLHYEIQIHSQAVDPRQFLWD"
     gene            504..1313
                     /locus_tag="VNU03_08560"
     CDS             504..1313
                     /locus_tag="VNU03_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWP74553.1"
                     /translation="MPSLSAKELTTDLLIRYGFQILGALVILGVGLLLARYVGNLTGR
                     WLERRSLEPPVRSLMVKTVRVVVLIFTLVVALDKFGFQVAPLVAGIGVAGLGVGIALQ
                     GVLGNLVAGLSIILTKPFRVGQWISIVGVYGQVSSIELFATTLLHPDQSRVIIPNRKI
                     VGEILHNHGTIRQLELAVGVSYATDLPKALAAARETLLANSRVLKTPAPMVGIASLGD
                     SAINLALGPWVAVPDYAPAQGELYQSVVERFRAAGIEMPFPQREVRLLGAA"
     gene            1352..2113
                     /locus_tag="VNU03_08565"
     CDS             1352..2113
                     /locus_tag="VNU03_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HWP74554.1"
                     /translation="MVLADQPVGLAIMAKAPRAGAVKTRLCPPLRPPEAAELARCFLL
                     DAVERAAGVVGVRPVLAYAPVEARGEFESVAPGFALLAQQGEDLGARQARLISEVLSL
                     GHQGALVIGTDSPTLPRECLDEAVSLVMAPDVDLVLGPTDDGGYYLIGLRAPCPALFE
                     GMPWSTSAVLPRTLDRAQHLGLRVACLPAWFDVDTKADLERLAAELAVTPGLLPRHTR
                     DFLARRISPGPAGDRSDDHASHATHGRGAVASRGD"
     gene            2120..2830
                     /gene="rpiA"
                     /locus_tag="VNU03_08570"
     CDS             2120..2830
                     /gene="rpiA"
                     /locus_tag="VNU03_08570"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HWP74555.1"
                     /translation="MATPTTVGQDQGPLERLASSALEYVRDGMRVGLGSGRAASAFVR
                     ALGARVREGLAVRGVPTSENTGRLARDLGVPLSGLDEGLIDLTVDGADEVDPRLDLIK
                     GYGGALVRERIVAAASRRVVILVGREKLVPVLGTRGRLPVEVVPFAAPLTIRELADLG
                     CRPVLRIEEGRPFVTDNGNWTVDCGVRAIEAPAAFARDLRALPGVVDTGLFLGVPDLV
                     LVADGAVIREMRRGDPAA"
     gene            2847..3602
                     /gene="pgl"
                     /locus_tag="VNU03_08575"
     CDS             2847..3602
                     /gene="pgl"
                     /locus_tag="VNU03_08575"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWP74556.1"
                     /translation="MRAIRRYPDAEAVSRAAAQDLVEVARAAIAARGRFCVALSGGST
                     PRRMYEILAEGPRWARLEWDRVEFFWGDERAVPPDHPESNYGAAAGALLSKLAIPPER
                     VHRILGELPDAEEAGARYQAELARVFAAPVDGLPPIFDLVLLGMGPDGHTASLFPYSQ
                     ALTERRRWVVGHHVARLGKARITMTPPILNRAAEVRLLVTGADKAGVLREALVGAREP
                     ERLPVQLVAPEAGRLVWMLDRAAAAELPADRPV"
     gene            complement(3645..5105)
                     /locus_tag="VNU03_08580"
     CDS             complement(3645..5105)
                     /locus_tag="VNU03_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP74557.1"
                     /translation="MSVPEPDWAGLRHAVTTRHRPIMEGLAEFLRLDTVSQKPERVRT
                     GAEWLSRAMRARGLEARVLETGGNPAVFGALPAPRAERTLLIYCHYDVKPAPPAGWLQ
                     PSPFEPVLRAGPAEEGAPALELGGVADEALPRHQLYGRGASDDKGPIWAHLTAVELMR
                     ARGISPRVAVKFIFDGEEEMGSQHFGPFTERHRDLLAADLVLVTDGPKDASGRPTVAF
                     GARGILSLELTLESARRDVHSGNFSVPNPAWRLVGLLASMAAPDGTPLVEGLEDGVVP
                     PTAAERDLMGRIPLDRAAIERELGVALPPQYLERLMFRSTLSIRGLKSGFTGQEAQTI
                     IPHQATVAFDARLVKNQRVETVYRRILEHIRGQGFTVIESPDAPIPDDLRGRAIREVE
                     RRGYDPAKTAADLPVSRRVIEVVEQAHGGEPAVVLPTMGGSVPLWAFTDILKLPTIVV
                     PYANANNRQHSPNEHLRLDHLYQGVLTTAHLLHGLG"
     gene            5239..6897
                     /locus_tag="VNU03_08585"
     CDS             5239..6897
                     /locus_tag="VNU03_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP74558.1"
                     /translation="MSTLSDRLGFRRTPRALLALLLALDLLAANAALLTIARPAAAQQ
                     SEADVFVAQAILAYDARRYDEALSLLKEALAQDPNNVEALYYSGLVLMAQQKTDQAVE
                     VLEKARARSPRDLSIQFLLGVAYFALERYDQAEAPLTQVFNERPQTEGVGYYVGLIRY
                     RKKDYQGALKAFRAETSKNPNIQQLVKFYSGLTLAILGLPEQAVAEVDAALRLQTGSS
                     LTGPAERIRDTLASARDAERRFHAEVRVGFLYDSNVAVVPQPSHDPTAESLRGRKTAT
                     PGELGALRLDYAWLRRGPWESTVTYSFFQTYNNRLPSFNVQNHLVAGAFSYRAAIGSM
                     PFQVGAQYSWDYLSLGGDDFLQRHTATLFGTLVENKGNLTTLLARFQNKDFYATPGQL
                     RDEDRDAKNWMVGGIHVFRFAEDRHYIRVGYQFDYENADGRNYTYAGNRFLAGGQYTL
                     PWGSTRLKYDFDLHQRHYLHANTLLPAVNPGTREREDLEQNHVFRIEQVLAKNLGPTS
                     IGCAPTAPCPLTLAAEYQRTIADSNLAVYAFNRNVWSLTLSWQY"
     gene            complement(6910..>7417)
                     /locus_tag="VNU03_08590"
     CDS             complement(6910..>7417)
                     /locus_tag="VNU03_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74559.1"
                     /translation="APLLSLTRSAALATGSSLADISGGASVKLGQLASLTASSLTVQG
                     HGLSLSGGATMTVAGDLFRIANGSTLTISNGALLNLSGGSSLTVAGALINFIGTGNTL
                     NITNNLCGNGCAMIGSLPVFVPAGVPINQAIILSNPILNLTGNHLNITAGSAAIVVTG
                     GAQVKQGP"
BASE COUNT         1029 a         2569 c         2690 g         1129 t
ORIGIN      
        1 cccgccccat gctcctgtcg ctgccgctgc gctggccggt gcgcgggccg ctcaactcgc
       61 ggttcggcat gcgccagtcc ccgtggaccg gcgagcagga gttccaccgc ggcctcgaca
      121 tcagcgcgaa tcgggggacc gcggtcctgg ccccggcctc cggcaccgtg tacttcgcgg
      181 gcagcagcgg cgagtacggc accaccgtca tcctcgatca cggccacgag ctgcgctcgc
      241 tctacggaca cctgcaggag atccgcgtga agccgggcga ccgcatcgac cggggacagg
      301 tcatcgcgct caccggaagc accggccgca cctccggccc gcacctgcac tacgagatcc
      361 agattcacag tcaggcggtg gatcctcgcc agttcctctg ggactagccg agccgcttcc
      421 tcctccgggg cctcgccccg gccccgcagt tgtgctgtga tcctcctcac tcgacgtcac
      481 tcgactcacc gaggaggact cgcatgccgt cgctgtccgc gaaggagctc acgaccgatc
      541 tgctgatccg ctacggcttc cagatcctgg gcgcgctcgt catcctgggg gtgggcctgc
      601 tcctggcccg ctacgtgggc aatctgaccg ggcgctggct cgagcgccgg tcgctcgagc
      661 cgccggtgcg cagcctcatg gtgaagacgg tgcgggtggt ggtgctgatc ttcaccctcg
      721 tggtggccct cgacaagttc ggcttccagg tggccccgct ggtggcgggc atcggggtgg
      781 ccggcctcgg cgtcgggatc gcgctgcagg gcgtgctggg caacctggtg gccgggctca
      841 gcatcatcct caccaagccc ttccgggtgg gccagtggat ctcgatcgtg ggggtgtacg
      901 ggcaggtgag cagcatcgag ctgttcgcca ccacgctgct gcatccggac cagtcgcggg
      961 tgatcatccc caaccggaag atcgtcggag agattctcca caaccacggg acgatcagac
     1021 agctcgagct cgcggtcggg gtgagctacg cgaccgatct gccgaaggct ctggccgcgg
     1081 cccgcgagac cctcctggcg aactcgcgcg tgctcaagac gccggcgccg atggtgggca
     1141 tcgcgagcct cggtgactcg gccatcaacc tggcgctggg accctgggtc gcggtcccgg
     1201 actacgcccc ggcgcaggga gagctgtacc agtcggtggt ggagcgcttc cgggccgccg
     1261 gcatcgagat gccgttccca cagcgggagg tccgcctgct cggcgccgcc tgacgggagc
     1321 gggcgctcca ccggcgtggc atgatggccc catggtgctt gcagatcagc cggtgggcct
     1381 ggcgatcatg gcgaaggcgc cccgggcggg ggcggtcaag acgcggctct gcccgccgct
     1441 gcgcccgccc gaagcggccg agctggcccg atgcttcctc ctggacgcgg tggagcgggc
     1501 ggcgggtgtg gtcggcgtcc ggccggtgct cgcgtacgcc ccggtggagg cacggggtga
     1561 gttcgagagc gtcgcgccgg gattcgccct gttggcccag cagggggagg acctgggcgc
     1621 ccggcaggcg cggctcatct ccgaggtcct gtcccttgga caccaggggg ccctcgtgat
     1681 cggcacggat tcgccgaccc tgcctcggga gtgcctcgac gaggcggtga gcctggtgat
     1741 ggcgcccgac gtggacctgg tcctcggacc caccgacgac ggcggctatt acctgatcgg
     1801 gctccgggct ccgtgcccgg ccctgttcga gggcatgccc tggagcactt cggcggtact
     1861 gccccgcacg ctcgaccggg cccagcacct cgggctccgg gtggcgtgtc tgcccgcgtg
     1921 gttcgatgtc gacaccaagg ccgacctgga gcgcctggcc gccgagctgg cggtgactcc
     1981 cgggctcctg ccgcgccaca cccgtgattt tctcgcgcgg cgaatctcgc cgggccccgc
     2041 cggcgacaga tctgatgatc atgcaagcca cgcgacgcac ggcagaggag ccgtggcgtc
     2101 gcgcggtgac tgaccgagcg tggcgacgcc cacgacggtc ggccaggacc agggtccgct
     2161 cgagcggctg gcgagcagcg cgctcgaata cgttcgcgac gggatgcggg tcggcctcgg
     2221 ctccggccgg gccgcatcgg cgttcgtgcg cgcgctgggt gcgagggtga gggaagggct
     2281 cgcggtgcgg ggcgtgccga cgtcggagaa caccgggcga ctggcccgag atctcggggt
     2341 gcccctgtcg ggactcgacg agggcctcat cgacctgacg gtcgacgggg cggacgaggt
     2401 cgacccgcgg ctcgacctga tcaagggcta tggcggcgcg ctggtgcggg agcggatcgt
     2461 cgcggcggcg tcgcggcggg tcgtcatcct ggtgggccgc gagaagctcg tgccggtgct
     2521 cgggacccgc ggtcggctgc cggtcgaggt cgtaccgttc gcggccccgt tgaccatacg
     2581 cgagctggcc gatctcggct gccgcccggt gctccggatc gaggaaggac ggccgttcgt
     2641 gaccgacaac ggcaactgga ccgtggactg cggcgtgcga gccatcgagg cgccggccgc
     2701 cttcgcccgc gacctccggg cgctgcccgg agtggtggac acggggctct ttctcggcgt
     2761 gccggacctg gtgctggtgg ccgatggtgc ggtcatccgg gagatgaggc gaggagatcc
     2821 ggcggcgtga gacggctccg gcagcggtga gggcgatccg gcggtacccc gacgccgagg
     2881 cggtgagccg ggcggcggcc caggacctgg tggaggtggc gcgcgcggcg atcgcggccc
     2941 gtggacggtt ctgcgtggcg ctctccggtg ggtcgacgcc ccggcgcatg tacgagatcc
     3001 tggccgaggg tccacgctgg gcgcgactcg agtgggaccg ggtggagttc ttctggggcg
     3061 acgagcgcgc ggttccgccc gaccacccgg agtcgaacta cggggcggcg gccggcgctc
     3121 tgctcagcaa gctcgccatt cctcccgagc gcgtccaccg aatcctgggc gagctgccgg
     3181 atgccgagga ggcgggcgcg cgctaccagg cggagctggc ccgtgtgttc gccgcgcccg
     3241 tcgacgggct gccgccgatc ttcgatctgg tcctgctcgg catgggcccg gacggtcaca
     3301 ccgcctcgct gtttccctat agccaggcgc tcaccgagcg acgccgatgg gtcgtcggcc
     3361 accacgtggc ccgtctcggc aaggcgcgga tcaccatgac gccgccgatc ctgaatcgcg
     3421 ccgccgaggt ccgcctgctg gtgaccggag ccgacaaggc cggcgtcctc cgagaggccc
     3481 tcgtgggagc gcgggagccg gagcgccttc cggtacagtt ggtggcgccc gaggccggcc
     3541 gcctggtctg gatgctcgat cgggccgccg ccgctgagct gcccgccgac cgtccggtct
     3601 gatcgagccc caccaccgcg gggcccgctt cgcgcggagt aggcctagcc cagcccgtgg
     3661 agcaggtgcg cggtggtgag cacaccctgg tagaggtgat cgaggcgcag atgctcgttg
     3721 gggctgtgct gccggttatt ggcgttggcg tatggcacca cgatggtcgg gagcttcagg
     3781 atgtcggtga aggcccagag tggcacgctg cctcccatgg tcggcaggac caccgccggc
     3841 tcgccgccgt gggcttgctc caccacctcg atcacccggc gggagaccgg caggtcggcg
     3901 gcagtcttgg ccggatcgta gccccgccgc tcgacctccc ggatcgcgcg cccgcgcagg
     3961 tcgtcgggga tcggcgcgtc gggcgattcg atcaccgtga aaccctggcc ccggatgtgc
     4021 tcgagaattc gccggtatac cgtctcgacg cgctggttct tcacgaggcg cgcgtcgaac
     4081 gccaccgtcg cctggtgcgg gatgatcgtc tgggcttcct gaccggtgaa gccgctcttg
     4141 aggccgcgga tgctcagggt ggagcggaac atgagccgct cgaggtactg gggagggagc
     4201 gcgaccccga gctcccgctc gatcgcggcc cggtcgagcg ggatgcggcc catgaggtcg
     4261 cgctccgccg cggtgggcgg gaccacgccg tcctccagac cctccacgag cggagtgccg
     4321 tcgggcgcgg ccatcgaggc gagcaggccc accagccgcc aggccgggtt gggaacgctg
     4381 aagttgcccg agtgcacgtc gcggcgggcg gactcgagcg tcagctcgag cgagagaatc
     4441 ccgcgcgcgc cgaaggccac ggtgggccgg ccgctggcgt ccttcggccc atcggtcacc
     4501 aggacgaggt cggcggccag caagtcacgg tgccgctcgg tgaaggggcc gaagtgctgg
     4561 ctgcccatct cctcctcgcc gtcgaagatg aacttgaccg ccacccgggg gctgatgccc
     4621 cgggcacgca tcagctcgac cgcggtgagg tgcgcccaga tcggcccctt gtcatcggag
     4681 gcgccacggc cgtagagctg gtggcggggt agggcctcgt cggcgacccc gcctagctcc
     4741 agggcagggg cgccctcctc ggcaggcccg gcccggagga ccggctcgaa tggggaaggc
     4801 tggagccagc cggccggggg ggccggcttg acgtcatagt ggcagtagat caggagcgtg
     4861 cgctcggccc tcggggccgg gagggccccg aaaaccgctg ggttgccgcc cgtttccaga
     4921 acgcgagcct cgagcccccg cgcccgcatg gcccggctga gccactcggc tcccgtgcgt
     4981 acgcgctcgg gcttctggct caccgtatcg aggcggagga actcggcaag gccctccatg
     5041 atggggcggt ggcgggtggt gaccgcgtga cggaggcctg cccaatcggg ctcggggacg
     5101 ctcatcggcc caccttaccc acggatgacg ctctgcgcta gtccctttcg gcaggtgcgc
     5161 aaaattggac tatccggggc tcttctaggt agagtagcgg ctcgcgtgac ggccggctcg
     5221 gtccggtccc acgccccgat gagcacgctc tccgatcgac tcggcttcag gcggacgcca
     5281 cgcgcgctgc tggcgctgct actcgcgctg gatctgctcg ccgccaacgc cgctctcctg
     5341 acgatcgccc ggccggccgc ggctcagcag tccgaggccg acgtgttcgt cgcccaggcc
     5401 atcctggcgt acgacgcccg gcgttacgat gaggccctgt ccctactcaa ggaggccctg
     5461 gcccaggacc ccaacaacgt cgaggctctc tactacagcg gcctcgtcct gatggcccag
     5521 cagaagacgg accaggccgt cgaggtgctg gagaaggccc gggcccggtc gccccgggat
     5581 ctctcgatcc agttcctgct cggggttgcc tacttcgccc tcgagcgcta cgaccaggcg
     5641 gaagctccgc tcacccaggt cttcaacgag cggccccaga cggagggcgt cggctactac
     5701 gtcggcctga tccggtaccg gaagaaggac tatcaggggg cgctgaaggc cttccgggcc
     5761 gagacctcga agaacccgaa catccagcag ctggtgaagt tctactccgg gctgaccctc
     5821 gccatcctgg gcctcccgga gcaggcggtg gccgaggtgg acgcggccct gcggctgcag
     5881 accgggtcgt cactcaccgg cccggccgag cgcatccgcg acacgctggc ctccgcccgc
     5941 gacgccgagc ggcgattcca cgccgaggtg cgggtgggct tcctctacga cagcaacgtg
     6001 gcggtggtgc cgcagccgag ccacgacccg acggcggagt cgctgcgggg tcgcaagacc
     6061 gcgacgccgg gagagctggg cgccctgcgg ctcgactacg cgtggctgcg ccgcgggccc
     6121 tgggaatcga ccgtcaccta ctcgttcttc cagacgtaca acaaccggct gccgtccttc
     6181 aacgtgcaga accatctggt cgcgggcgcc ttctcgtatc gcgcggcgat cggctccatg
     6241 cccttccagg tgggcgcgca gtactcgtgg gactacctat cgctcggggg cgacgacttc
     6301 ctccagcgcc acaccgcgac cctgttcggc acgctggtgg agaacaaggg caacctgacc
     6361 acgctcctgg cccggttcca gaacaaggat ttctacgcga ccccggggca gctccgcgac
     6421 gaggatcgcg acgcgaagaa ctggatggtc ggcgggatcc acgtcttccg gttcgcggag
     6481 gaccggcact acatccgggt gggctaccag ttcgactacg agaacgcgga cggccggaac
     6541 tacacgtacg cgggcaaccg cttcctggcc ggcgggcagt acacgctccc gtggggatcg
     6601 acccggctga agtacgactt cgatctgcac cagcgtcact acctgcacgc caacacgctg
     6661 ctgccggcgg tcaacccggg gacccgggag cgcgaggatc tcgagcagaa ccacgtgttc
     6721 cgcatcgagc aggtcctggc caagaacctg gggccgacct ccatcggctg cgcgccgacc
     6781 gcgccctgtc cgctcacgct cgccgccgag taccagcgga ccatcgcgga ctccaacctg
     6841 gcggtctacg ccttcaaccg caacgtctgg tcgctgaccc tgtcctggca gtactgaggc
     6901 ggggtggggc tacgggccct gcttgacctg cgcgccgccg gtcacgacga tcgcggcgga
     6961 gccggcggtg atgttgaggt ggttgccggt caggttcagg atcgggttgc tgaggatgat
     7021 cgcctgattg atcggcacgc ccgccggcac gaagaccggc aggctcccga tcatcgcgca
     7081 gccgttgccg cagaggttgt tcgtgatgtt gagggtgttg ccggtgccga tgaagttgat
     7141 gagcgcgccg gcgacggtca gggatgatcc gccgctcaaa ttcagcagcg cgccgttgct
     7201 gatggtcagc gtgctgccgt tggcgatgcg gaagagatcc cccgcgaccg tcatggtggc
     7261 gcccccgctc aggctcaggc cgtgcccctg gacggtcaag ctgctcgcgg tcagcgaggc
     7321 gagctggccg agcttgacgc tcgcgccccc cgagatgtcg gcgaggctgc ttccggtcgc
     7381 cagcgccgcg ctgcgggtca agctgagcag aggggcg
//
LOCUS       DATMCV010000367         2691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_682686, whole genome shotgun sequence.
ACCESSION   DATMCV010000367 DATMCV010000000
VERSION     DATMCV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682686"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VNU03_08595"
     CDS             <1..202
                     /locus_tag="VNU03_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HWP74560.1"
                     /translation="ELVEEVVRSMGKAIRPERVLFARELPKTRSAKIMRRVVRATYLG
                     RDPGDLSSLDNPGGVKAIADAS"
     gene            complement(294..1004)
                     /locus_tag="VNU03_08600"
     CDS             complement(294..1004)
                     /locus_tag="VNU03_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HWP74561.1"
                     /translation="MPPEVQALLFDTYGTVVDWRSGVLEALVALGRARGLSIACEQLL
                     ADWDPRPIMDRVNSGELPWMTMEDVHRQALGESLARHGITGLTEREIERLVQARWRLA
                     PWPDAVRGLTRLKQRYVISPLSNASFTGMVRLAKFAGLPWDCVITAENARRYKPHPDV
                     YRTAVALLGWRPDQVMLVAAHNYDLRAAQGEGLRTAFVPRPREYGPGQTTDLAPEGSW
                     DVVAKDFLDLAEKLGARD"
     gene            complement(994..>2691)
                     /locus_tag="VNU03_08605"
     CDS             complement(994..>2691)
                     /locus_tag="VNU03_08605"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c/FTR1 family iron permease"
                     /protein_id="HWP74562.1"
                     /translation="PARPERAALEATAAGLLGAIRDKRPGEEVAAISGDLRRRIIGLY
                     DVRVTPRQVPDLAPAAADFTTHCAVCHGAAGRGDGPGAKGLTPPPADLTDAARMGEHS
                     LFGLYNTITLGIKGTAMTGFAPLGEGQRWALAFYVSTLATPPAERERGAQLWKSGAGR
                     GELRDLRSLVMATPKEVAARAGGDGVAVLAYLRGDPAALAAVRESPLDLSTRLLVESL
                     AAYRQGDATRAHQLAITSYLEGFELVEVQLSAVDGALKTRVEGEMLRYRTLIQSHAPR
                     DAVEAEARTILGLLETTRQRLDAARLSPATTFSSALVILLREGLEAILVLAALVAMLI
                     KSGRREALRYVHAGWIAALALGAVTWVAASYVVTLSGASREVTEGVTALIAAGMLLYV
                     GFWMHRHAHAARWKTFIETQVQAALSGRTRWALASIAFLAVYREAFETVLFYQALWIE
                     AGPSGHVAVAGGFAAAAAGLVLLAWLILKMGLRLPVGWFFGVGSALMALLAVVLAGKG
                     IAALQHAGRLPVEPLDLPTIPSLGVYPTWQGVLTQLALVLLILTAFTYSRRHPRHAA"
BASE COUNT          377 a          979 c         1004 g          331 t
ORIGIN      
        1 cgagctggtg gaggaggtcg tgcgctcgat gggcaaggcg atccggccgg agcgcgtgct
       61 cttcgcgcgc gagctgccga agacccgcag cgccaagatc atgcgccgcg tggtgcgcgc
      121 gacctacctc gggcgcgacc cgggcgacct ctcctcgctc gacaacccgg gcggcgtcaa
      181 ggccatcgcc gacgcctctt gaagagcctt cgtccctctg accctctccc ctcaggggag
      241 agggcagggt gaggggtggt cagggtgagg ggcgcatcag gctgaggggc gggtcaatcc
      301 ctcgccccca gcttctccgc caggtcgagg aagtccttcg ccaccacgtc ccagctcccc
      361 tccggcgcca gatcggtcgt ctgccccggg ccgtactcgc gcggccgcgg cacgaacgcg
      421 gtgcgcagcc cctcgccctg cgccgcgcgc agatcgtagt tgtgcgcggc gaccagcatc
      481 acctggtccg gccgccaccc gagcaatgcc accgcggtgc gatagacgtc cgggtgcggc
      541 ttgtagcggc gcgcgttctc ggcggtgatc acgcagtccc aggggagccc ggcgaacttg
      601 gccaggcgca ccatgccggt gaagctcgcg ttggagagcg ggctgatcac gtagcgctgc
      661 ttcaggcgcg tcagcccgcg gaccgcgtcg ggccagggcg cgaggcgcca ccgggcctgg
      721 accaggcgct cgatctcccg ctcggtgagc ccggtgatgc cgtggcgggc cagcgactcg
      781 cccagggcct gccgatggac gtcctccatc gtcatccagg ggagctcgcc cgagttcacg
      841 cgatccatga tcggccgcgg gtcccagtcg gccaggagct gctcacacgc gatcgagagc
      901 ccgcgcgcgc ggcccagggc caccagcgcc tcgagcaccc cgctccgcca gtcgacgacg
      961 gtgccgtagg tgtcgaagag gagcgcctgg acctcaggcg gcatgccgtg ggtggcgccg
     1021 ggagtaggtg aaggcggtga ggatcagcag caccagcgcg agctgggtga gcacgccctg
     1081 ccacgtcgga tacacgccga gcgacgggat ggtgggcagg tcgagcggct cgacgggcag
     1141 ccggcccgcg tgctggagcg cggcgatgcc cttgccggcc agcaccaccg cgagcagcgc
     1201 catcagcgcc gagcccacgc cgaagaacca gccgaccggc agccgcagcc ccatcttgag
     1261 gatcagccag gccagcagca cgagccccgc cgcggctgcc gcgaagccgc ccgcgaccgc
     1321 cacgtggccc gacgggccgg cctcgatcca gagcgcctgg tagaagagca cggtctcgaa
     1381 ggcctcgcgg tagaccgcga ggaacgcgat cgaggccagc gcccagcggg tgcggccgga
     1441 cagcgcggcc tgcacctgcg tctcgatgaa cgtcttccag cgcgccgcgt gggcgtggcg
     1501 atgcatccag aagcccacgt agaggagcat gcccgccgcg atcagcgcgg tgaccccctc
     1561 ggtgacctcg cggctcgccc cgctcagcgt gaccacgtag gaggcggcga cccaggtgac
     1621 cgcgcccagg gccagcgcgg cgatccagcc cgcgtgcacg tagcgaagcg cctcgcgccg
     1681 ccccgacttg atcagcatcg cgacgagggc ggccagcacc aggatggcct cgaggccctc
     1741 ccgcagcagg atgacgagcg cgctggagaa cgtggtggcc ggcgagagcc gcgccgcgtc
     1801 gagccgctgc cgcgtcgttt cgagcaggcc gaggatggtg cgtgcctccg cctcgaccgc
     1861 gtcgcgcggg gcgtgggact ggatcagggt gcggtagcgg agcatctcgc cctcgacccg
     1921 ggtcttgagc gcgccgtcca ccgcgctcag ctgtacctcc accagctcga agccttccag
     1981 gtagctcgtg atcgcgagct ggtgcgcgcg cgtcgcgtcg ccctgccggt acgcggcgag
     2041 gctctccacg agaaggcgcg tgctcaggtc gaggggggac tcgcggaccg ccgcgagcgc
     2101 ggccggatcg ccgcgcagat aagcaagcac cgcgaccccg tcgccgcccg cgcgggccgc
     2161 cacctccttc ggggtggcca tcaccagcga tcgcagatcg cgcagctcgc cccgtccggc
     2221 cccgctcttc cacagctgcg cgccgcgctc ccgctccgcg gggggcgtgg cgagcgtgct
     2281 cacgtagaag gccagcgccc agcgctggcc ctcgccgagg ggcgcgaagc cggtcatggc
     2341 ggtgcccttg atgccgaggg tgatcgtgtt gtagaggccg aagaggctgt gctcgcccat
     2401 gcgggccgcg tcggtgagat cggcgggcgg cggggtcagg cccttcgcgc cgggcccgtc
     2461 gccgcggccg gccgcgccgt ggcagaccgc gcagtgggtg gtgaagtcgg cggcggcggg
     2521 cgccaggtcg gggacctgcc gcggggtcac gcgcacgtcg tagagaccga tgatgcggcg
     2581 gcggagatcg cccgagatcg ccgcgacctc ctcgccggga cgcttgtcgc ggatcgcgcc
     2641 caggaggccg gccgcggtgg cctcgagcgc ggcccgctcg gggcgggcgg g
//
LOCUS       DATMCV010000368         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_815893, whole genome shotgun sequence.
ACCESSION   DATMCV010000368 DATMCV010000000
VERSION     DATMCV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815893"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(531..1337)
                     /locus_tag="VNU03_08610"
     CDS             complement(531..1337)
                     /locus_tag="VNU03_08610"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP74563.1"
                     /translation="MPSIFNARDADSYEQTMGRWSRRLAPLFIEHAGVAAGERLLEVG
                     CGTGSLTFTLAGAVAFGELTAIDYADVYVAAARARNRDPRIHIEQGDAVALRFPDAHF
                     DRALSLLVLHFVSEPGAAVAQMRRVTRPGGVVAAAVWDSGGGQIAQRMFWDTAAMLDP
                     AAVEHRGRAMSKPVVAPGGLARVFRAAGLADVDERPLLIRMDHPDFADYWDPFLRGEG
                     PIGGYVVGLGEAERARLEQHVRAAYLAGAPDGPRSFAAVAWSCRGVVPRS"
     gene            1489..>2198
                     /locus_tag="VNU03_08615"
     CDS             1489..>2198
                     /locus_tag="VNU03_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74564.1"
                     /translation="MGDERTHAAPLGERERLAIVGLGLSPLGVGPIRMGRDVAEQVQR
                     VGREARVSGRIEERALGQATGLVEPAEPQRGPTQCVAGLGVEPDDSPPRLTLEELLAL
                     LESGHGQVDLVDLREGPRRAGHRVGQVQHDVPGPVGRDPVLDQGARPRPVPLEQVQPA
                     RGQMGHADRIGMARGLGEAERLRLVPGGPGETAELREAPDQEVAIVDRDRHGGPERLV
                     LRVGGQRREVLGGQLHHSL"
BASE COUNT          329 a          823 c          761 g          285 t
ORIGIN      
        1 tgatgcaata cagcgcgttg cccccggacg ggacctggcc gaggacctcc gcctcgcgct
       61 gaccggtcac gccgccttgc tcgttcaggc cctcgacccg gagccggacg tgggcggcgg
      121 tcatgggccg gtcattggac acgtgcccgc aaaccgcctg caggccactc cgcaccgggg
      181 ccacgtccca gcgtacctgc aggcccgccg ggccctctgc tccctcgccc tgcccccccg
      241 cggcagacag gaccagcaaa cccatcagag ccgcgcaccc gatccatcgt ctgagcatgt
      301 ttcctccgcg cgattgagat gatttcagcc cggcgctggg tgcgcacggc ctccggtcca
      361 gcaagaatcc gacatgcgaa cccttcgagc acccggccgc gtctagatag acagggaagc
      421 actcccggga gaaaccgaca tgttaaagga cgcgatgaag ctcgagcggt gggttgctgt
      481 cgtttccgtg ctcctcctcc tggccctggc cgagatgggg tggcgctgac tcacgagcgc
      541 ggtacgaccc cccggcagga ccaggcgacc gccgcaaagg agcgaggccc gtcgggtgcg
      601 ccggcgaggt acgccgcccg cacatgctgc tcgagccgtg cccgctccgc ttcgccgagc
      661 ccgacgacat aaccgccgat cggcccctcg ccgcggagga acgggtccca gtagtcggcg
      721 aagtcggggt gatccatccg gatcagcaag ggccgctcgt cgacgtcggc gaggccggct
      781 gcccggaaca cccgcgcgag cccgccggga gcgaccaccg gcttcgacat ggcgcggccg
      841 cgatgctcga ccgccgcggg gtcgagcatc gcggcggtat cccagaacat ccgctgggcg
      901 atctgcccgc cgccggaatc ccagaccgcc gcggcgacca ctccccccgg ccgggtgacc
      961 cgccgcatct gcgccaccgc cgcccccggc tcggagacga agtgcaggac caggagcgac
     1021 agcgcgcgat cgaagtgtgc gtcggggaag cgcagcgcga ccgcgtcccc ctgctcgatg
     1081 tggatgcgcg ggtcgcgatt cctggcgcgg gccgccgcga cgtagacgtc ggcgtagtcg
     1141 atggcggtca gctccccgaa ggcgaccgct ccggccaggg tgaaggtcag gctgccggtg
     1201 ccgcagccca cctccagcag ccgctcgccc gcggcgacgc cggcgtgctc gatgaacagc
     1261 ggcgccagac ggcggctcca gcgccccatc gtctgctcgt agctgtcggc atcgcgcgcg
     1321 ttgaagatcg acggcatggc ctaaggccct cctcgcccct tcctcggcat tcgtgagcca
     1381 gtacgtcatg tccatctccc ggtacatcat cgtcgcgatg gccagatgct cccgagccag
     1441 cgcgccctcg cctccgcgcc gggccagccg gccgagaccg agatagcagt gggcgacgag
     1501 cggacgcatg ccgcacccct cggcgagcgc gagcgccttg cgatagtggg cctcggcctc
     1561 tccccgctcg gcgtggggcc gatccggatg ggccgcgacg tcgccgagca ggtgcagcgc
     1621 gtgggccgcg aagcccgggt gagcgggaga atagaagagc gcgcgctcgg ccaggcgacg
     1681 ggcctcgtcg agccggccga gccgcagcgc ggcccgaccc agtgcgtggc aggcctcggc
     1741 gtggagcccg acgattcccc gccccgcctc acgctggagg agttgctcgc cctcctggaa
     1801 tcgggtcacg gccaggtcga cctcgtcgac ctgcgcgagg gcccacgccg agcaggccac
     1861 cgcgtggggc aggttcagca cgatgttccc ggtccggtag gccgcgaccc cgtgctcgat
     1921 cagggtgcgc gcccgcgccc agtccccctc gagcaggtgc agccagctcg cggccagatg
     1981 ggccatgccg accgcatagg catggcgcgt gggctcggcg aggcggagcg cctccgcctc
     2041 gtaccgggcg gcccgggaga aacggccgag ctgcgcgagg ctcctgacca ggaggtagcg
     2101 atcgtagatc gcgatcggca tggcggcccc gaacgactcg tactccgagt cggcgggcag
     2161 cgccgcgagg ttctcggtgg ccagctccac cactcgct
//
LOCUS       DATMCV010000369         3071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_885951, whole genome shotgun sequence.
ACCESSION   DATMCV010000369 DATMCV010000000
VERSION     DATMCV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885951"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..791
                     /locus_tag="VNU03_08620"
     CDS             123..791
                     /locus_tag="VNU03_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP74565.1"
                     /translation="MIRAGRLGASLTIALIIGLLAAAAAAQTKIPLTREQALTEIKSE
                     DLELRRRAAAWLGELGGPADAPALLGVLADPDEVVRVLAEHSVWQVWSRSGDKDIDGQ
                     FQVGVEQMSRGDGEAAVETFTRIIQRKPDFAEAWNKRATLYFLMGENEKSLHDCDEVM
                     KRNPAHFGALAGYGQIYLRLDQPERALTYFRRALRINPNMRGIEQVIPEIEQLLTERR
                     KGTI"
     gene            827..1522
                     /locus_tag="VNU03_08625"
     CDS             827..1522
                     /locus_tag="VNU03_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HWP74566.1"
                     /translation="MPLTTQEVEALKKITSPSVANAIETFKVRPREQGNVSSAIRALF
                     PEMGPMVGYAVPCVIRAEAGPIEGHRASTYGWWDFVLSIPAPRVIVVHDLDDPRGQGA
                     QWGEVQANIHRALGCVGVVTDGSVRDLDEVRALGFQFCAAHVSVSHANVHMVDFGIPV
                     KVGGLWIKPGDLIHGDQHGVCTIPPEIAPKIPEAIAKVEADEREIIKVCQSPGFTADK
                     LKELFKRIRPGTY"
     gene            1577..2011
                     /locus_tag="VNU03_08630"
     CDS             1577..2011
                     /locus_tag="VNU03_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP74567.1"
                     /translation="MIEIRLPRVADSVGCTTLAAQVIGEERAPAFIRSHMERHHLIVA
                     EAEGEVVGFIAYRLDWFQCTFVSLVVVREDFRRKSIAREFFKSVEVVSPTPRLFSSTE
                     ETNVASIRMHTALGFAPSGHIDNLPQGVRELLFYKRIPPRGA"
     gene            2025..2486
                     /locus_tag="VNU03_08635"
     CDS             2025..2486
                     /locus_tag="VNU03_08635"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HWP74568.1"
                     /translation="MPEKTTLLSGAETEFVALKRAINGLGEAQMREVWLGTWSARDIV
                     AHISGWHRELAPALERMAKGERPIPEGVSYDDVDAWNARFADAKKMAATADILKELDA
                     SHAAFLQAAGSVPEERYVPGKTAHKIVDFNSRHHYQEHRAQIEEWRKRKGI"
     gene            2495..>3071
                     /locus_tag="VNU03_08640"
     CDS             2495..>3071
                     /locus_tag="VNU03_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP74569.1"
                     /translation="MGRIREMAEQGWSGAVEPYALWTPTGAAEEIAPGVFFLHAFANV
                     TVIRAGDGVVLIDTSSQAARARTFAAVRAIDSAPVRAAIYTHGHADHAFGLPPFLAEA
                     EEQGWPRPRIVAHRDVAARFDRYRATAGYNGLINARQFSRPPAWPTEYVYPDTTYERT
                     LRFEADGLALELEHARGETDDHTWLWWPERRV"
BASE COUNT          492 a         1105 c         1059 g          415 t
ORIGIN      
        1 gtctcgaaga agacggacta cgtggtggtc ggcgaggacg cgggctcgaa ggcggaggac
       61 gctaagcgcc tcggggtggc cacgctcgac gaggacgcgt tcctcaagct ggtgggacgc
      121 tcgtgatccg cgccggccgc ctcggagcga gcctcaccat cgccctgatc atcgggctgc
      181 tcgcggcggc ggccgccgcc cagaccaaga tccccctcac ccgcgagcag gcgctgaccg
      241 agatcaagag cgaggacctc gagctgcgcc gccgcgcggc cgcgtggctg ggcgagctgg
      301 gcggaccggc cgacgccccc gccctgctcg gggtgctggc cgaccccgac gaggtggtgc
      361 gcgtcctcgc cgagcactcg gtctggcagg tgtggagccg ctccggcgac aaggacatcg
      421 acgggcaatt ccaggtgggc gtggagcaga tgagccgggg cgacggcgag gccgcggtgg
      481 agaccttcac ccgcatcatc cagcgcaagc ccgacttcgc ggaggcctgg aacaagcgcg
      541 ccacgctcta cttcctgatg ggcgagaacg agaagtcgct ccacgactgc gacgaggtca
      601 tgaagcgcaa cccggcccac ttcggagccc tggccggcta cgggcagatc tatctccgcc
      661 tcgaccagcc ggagcgtgcg ctcacctact tccgccgggc tctccgcatc aacccgaaca
      721 tgcgcggcat cgagcaggtc atccccgaga tcgagcagtt gctgacggag cgacgaaagg
      781 gcactatctg acccgatcca gccgtcaaac ccacagggag gacaccgtgc cgctcactac
      841 ccaagaggtc gaagcgctca agaagatcac cagccccagc gtcgccaacg ccatcgagac
      901 cttcaaggtg cggccgcgcg agcagggcaa cgtctcctcg gccatccgcg cgctcttccc
      961 ggagatgggg ccgatggtgg gctacgccgt cccctgcgtc atccgcgccg aggctggccc
     1021 catcgagggc caccgggcca gcacctacgg ctggtgggac ttcgtgctca gcatcccggc
     1081 gccgcgggtg atcgtggtcc acgacctcga cgacccgcgg ggtcagggcg cccagtgggg
     1141 cgaggtgcag gccaacatcc accgcgcgct cggctgcgtg ggcgtcgtca ccgatggctc
     1201 ggtgcgcgat ctggacgagg tgcgcgcgct cggcttccag ttctgcgccg cccacgtgtc
     1261 ggtctcccac gccaacgtgc acatggtgga cttcggcatc ccggtcaagg tcggcggcct
     1321 ctggatcaag ccgggcgacc tgatccacgg cgaccagcac ggggtctgca cgatcccgcc
     1381 cgagatcgcg cccaagatcc cggaggccat cgccaaggtc gaggccgacg agcgcgagat
     1441 catcaaggtc tgccagagcc ccggcttcac cgccgacaag ctcaaggagc tcttcaagcg
     1501 catccgaccc ggtacctact aggcgcctcc ggttgagcgg gcgccccggc gcgcttacaa
     1561 tgcgccgggg cgccccatga tcgagattcg actcccgcgc gtggcggatt cggtgggctg
     1621 cacgaccctc gccgcgcagg tcatcgggga ggagcgggcg cccgccttca tccgctcgca
     1681 catggagcgg caccacctga tcgtggccga ggcggagggt gaggtggtgg gcttcatcgc
     1741 ctaccggctg gactggttcc agtgcacctt cgtgagcctc gtggtggtcc gcgaggactt
     1801 ccggcggaag agcatcgccc gcgagttctt caagtcggtc gaggtggtct cgcccacccc
     1861 gcgcctcttc tcgtccaccg aggagaccaa cgtcgcctcg atccgcatgc acaccgcgct
     1921 cggcttcgcg ccgagcggcc acatcgacaa cctgccccag ggcgtccggg agctgctctt
     1981 ctacaagcgc atcccgcccc gcggggcctg agacggaggc cacgatgccc gagaagacga
     2041 cgctgctgag cggggccgag accgagttcg tggcgttgaa gcgagcgatc aacgggctcg
     2101 gcgaggcgca gatgcgcgag gtgtggctcg gcacgtggag cgcgcgcgac atcgtcgctc
     2161 acatctcggg ctggcatcgt gagctggcgc cggcgctcga gcgcatggcg aagggggagc
     2221 gcccgatccc ggagggcgtc tcctacgacg acgtggacgc ctggaacgcc cgcttcgccg
     2281 acgccaaaaa gatggcggcc accgccgaca tcctgaagga gctggacgcc tcccacgcgg
     2341 ccttcctgca ggccgccggg agcgtgccgg aggagcggta cgtgcccggc aagaccgccc
     2401 acaagatcgt cgacttcaac agccgccacc actaccagga gcaccgggcg cagatcgagg
     2461 agtggcgcaa gcgcaagggc atctagccgc cggcatgggc cgcatccgcg agatggccga
     2521 gcaggggtgg tcgggcgcgg tcgagcccta cgcgctctgg acgccgaccg gggccgccga
     2581 ggagatcgcg cccggcgtct tcttcctgca cgccttcgcc aacgtcacgg tgatccgcgc
     2641 gggcgacggc gtcgtgctga tcgatacctc gagccaggcc gcgcgggccc ggaccttcgc
     2701 ggcggtccgc gccatcgatt ccgcgccggt gcgcgccgcg atctacacgc acgggcacgc
     2761 cgatcacgcc ttcgggctgc cgcccttcct ggccgaggcc gaggagcagg gctggccgcg
     2821 gccgcggatc gtggcgcatc gggacgtcgc cgcgcgcttc gatcgctatc gcgccaccgc
     2881 gggctacaac ggcctgatca acgcgcgcca gttctcgcgc cccccggcct ggcccaccga
     2941 gtacgtctac ccggacacga cctacgagcg caccctgcgc ttcgaggccg acggcctggc
     3001 gctcgagctc gagcacgccc ggggcgagac cgacgatcac acctggctct ggtggcccga
     3061 gcgccgggtg c
//
LOCUS       DATMCV010000370         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_219722, whole genome shotgun sequence.
ACCESSION   DATMCV010000370 DATMCV010000000
VERSION     DATMCV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219722"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VNU03_08645"
     CDS             <1..353
                     /locus_tag="VNU03_08645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP74570.1"
                     /translation="LEALLADAWALVAPSLWAEPLGFVAIEAIVRDVPVIASELGGFG
                     ETIERDSSGLLFANGDETALYEQLARVAHRQAFPGHSLPADARRQVADRHDLGRHVGR
                     VRAIFRGLTPAGPG"
     gene            571..>2174
                     /locus_tag="VNU03_08650"
     CDS             571..>2174
                     /locus_tag="VNU03_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWP74571.1"
                     /translation="MFATPGPLDFVAVATAMREFWERERVFSRLVAQNRGGRRFSFLD
                     GPITANNPMGIHHAWGRTFKDVVQRYRALCGYEQRFQNGFDCQGLWVEVEVEKTLGFN
                     SKREIEAYGLERFSRACRERVTHFARVQTEQSQKLGQWMDWPSSYFTMSDPNIECNWT
                     FLRRCHERGWLYMGHRPMPWCTRCGTSISQHEMLDAYAELTHESVTVALPLRDRPDHR
                     LLVWTTTPWTLPANVAVAVHPELDYVECALADTVYYVAAGLAGRYPSLGPPRRTVKGA
                     ELVGLAYVGPFDDLPVQRDVEHRVIPWDEVSAAEGTGIVHIAPGCGLEDFDLGRRHGL
                     PVIAPVDPEGRYLDGLGALSGTPVADAAGPITRELKERGLLFARAAYRHRYPTCWRCG
                     QELIFRVADEWFLAAAEIRPLARAANERVTWLPGHMQLRMDDWLANMGDWCISRKRYW
                     GLPLPFYPCECGRLTVVGSRRELRELATDPAAVDAVPELHRPWLDGILVRCPHCARSV
                     RRIPEVGDCWLDAGIVPFSTLGYLEDR"
BASE COUNT          290 a          804 c          735 g          345 t
ORIGIN      
        1 ccctcgaagc cttgctcgcc gacgcgtggg ctctcgtcgc gccgtccctc tgggccgagc
       61 cgctcggctt cgtcgcgatc gaggcaatcg tgcgcgacgt cccggtgatc gccagcgagc
      121 tcggcggctt cggcgagacc atcgaacggg actcgagcgg gctcctcttc gccaacggcg
      181 acgagaccgc gctctacgag cagctggccc gggtcgcgca ccgccaggcc ttccccggcc
      241 actccctccc cgcggatgcg cgccggcagg tggccgaccg gcacgacctc gggcgccacg
      301 tcggccgcgt gcgggcgatc ttccgcgggc tgaccccggc cggcccggga taggcggaga
      361 ctcgccccgt cccttgccgg atatcgcggt tgtgctattc tctggtcatg ttccgaatta
      421 gtcggcccgt cacgagcagg gagattcctt cgagctgacg gtccgcccag cagtctcacg
      481 tatcccacgg ccgtcagggt tcctggcggc cgtttagttt tcccgcaacg gccccggggt
      541 cgctctacac cgagagtagg ggaggcgagc atgtttgcca caccggggcc gcttgacttc
      601 gtcgccgtcg cgaccgccat gcgggagttc tgggagcggg agcgtgtctt ctcgcgtctc
      661 gtggcccaga accgcggagg gcgacgcttc tccttcctcg acggcccgat caccgcgaac
      721 aacccgatgg gcatccacca cgcgtgggga cgcaccttca aggacgtcgt ccagcgctac
      781 cgggccctgt gcggctacga gcagcgattc cagaacggct tcgactgcca ggggctgtgg
      841 gtggaagtcg aggtcgagaa gacgctgggg ttcaactcga agcgggagat cgaggcgtac
      901 ggactggagc gcttctctcg cgcctgccgc gagcgggtga cccacttcgc gcgggtccag
      961 accgagcagt cgcagaagct cggccagtgg atggactggc ccagctccta cttcacgatg
     1021 tcggacccca acatcgaatg taactggacc ttcctgaggc ggtgccacga gcggggctgg
     1081 ctctacatgg gacaccgccc gatgccgtgg tgcacgcggt gcggcacctc gatctcccag
     1141 cacgagatgc tggacgccta cgccgagctc acgcacgagt cggtgaccgt cgccctgccc
     1201 ctccgggacc gccccgacca tcggctcctc gtgtggacga cgacgccgtg gaccctgccc
     1261 gccaacgtgg cggtggcggt tcacccggag ctcgactacg tcgagtgcgc gctggcggac
     1321 accgtctact atgtcgccgc cggcctcgcc ggacgctacc cctcgctcgg cccgccgcgg
     1381 cgcaccgtca agggcgccga gctcgtgggg ctagcgtacg tcgggccgtt cgacgatctg
     1441 cccgtccagc gggacgtgga gcatcgggtg atcccgtggg acgaggtgtc cgccgcggag
     1501 ggcaccggca tcgttcacat cgcgccgggc tgcggcctcg aggacttcga cctcggccgc
     1561 cggcacggac tgcccgtcat cgccccggta gatcccgagg ggcgctacct cgacggcctg
     1621 ggtgccctct cggggacgcc ggtcgccgac gccgccgggc cgatcacccg cgagctcaag
     1681 gagcgcggcc tgctcttcgc gcgggccgcg taccgccatc gctatcccac ctgctggcgg
     1741 tgcggccagg agctgatctt ccgcgtcgcg gacgagtggt tcctcgccgc ggccgagatc
     1801 cgaccgctcg cccgggccgc caacgagcgc gtcacgtggc tcccgggcca catgcagctc
     1861 cggatggacg actggctcgc caacatgggc gactggtgca tctcgcgcaa gcggtactgg
     1921 ggcctgcccc tgcccttcta cccctgcgag tgcggacgtc tcaccgtggt cggctcgcgc
     1981 cgcgagctcc gcgagctggc caccgacccc gccgcggtcg acgcggtccc ggagctgcac
     2041 cgtccgtggc tcgacggcat cctcgtccgc tgcccccact gcgcccggtc cgtccgtcgc
     2101 atccccgagg tcggcgactg ctggctcgac gccggcatcg tcccgttctc gacgctgggg
     2161 tacctcgagg atcg
//
LOCUS       DATMCV010000371         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_219732, whole genome shotgun sequence.
ACCESSION   DATMCV010000371 DATMCV010000000
VERSION     DATMCV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219732"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="VNU03_08655"
     CDS             <1..1387
                     /locus_tag="VNU03_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74572.1"
                     /translation="MTRPLIAFATLLLSLALPVGIAHAGEDWPVDPGVAERPPDLPSW
                     LIASRLGRPLDDRSRDDAMGSSGLSDLGAVLLRQGPGAPINSDPLTLRTSGEDSTDLR
                     ALLGRGDRWHLMSFGAAAANRPQDMKVTAYGAVAYLPSLSSSGPDGSLGFGPATAPGA
                     AFGLGLDDPEEPVSLGFKGELAGLGLEGGAEYRSVGKRLERVVTGPASQKDREGTEVW
                     LAQTLGLLRLKLSQSDLSDNVDRDPAVPRTTKVQTAVSAQLAPRDWPIFGLTYATGDS
                     ERTWLTGEGRTRTVERQTFDSVAGSAYYGARWFDLSGTSTYSYSREPGRSDRDMNSLY
                     HDLSLTLRPVNVLSVTPSLSSGVDRYEWSSTRYQFGSASLLLTYTPPASRFSLWTLGA
                     YTTSQSTDRSVDGRTMSVSGGLACGLGRILGGQASVAVQAGYDRYVDGIYPDSSTRGA
                     FGLVLLKVAAF"
     gene            complement(1418..2260)
                     /locus_tag="VNU03_08660"
     CDS             complement(1418..2260)
                     /locus_tag="VNU03_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74573.1"
                     /translation="MRLPRVLHRIPRNQLALAAGMVMAAAVLASLLAPYLPLLDPDAV
                     DTRNRLRPPGSPGHLLGTDEFGRDMLARLVWGGRISLLAGFATASVSMLVGGLLGVHA
                     GYWGGRVDTVVMRLTDILMAFPYILLAIAIVAGLGPGLANAMVAIAIVGFPLYTRLAR
                     GLVLSLREREFVEAARALGSSDRSILRRHLLPHLLAPVVVAWSLDVGAKILATAGLSF
                     LGLGTQPPTADWGSMLATARTFVILRPHVALLPGLAIFVVVLSLNILGDALQDFLDPR
                     LRHG"
     gene            complement(2271..>3128)
                     /locus_tag="VNU03_08665"
     CDS             complement(2271..>3128)
                     /locus_tag="VNU03_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74574.1"
                     /translation="GDPAEIMLGSQASQEDVARLRADLGLDEPLPLQYARWMAHVLQG
                     DLGRSIRLRQPVLGEVMIRFRATLLLTTTALLLSTVCGIALGVLSAARPRSLVDRLST
                     LGSIFGASMPSFWLGLVLMVCFSLWLGWLPASGMYGAHGTGGLADLLSHLALPAVTLA
                     AASTTIIARLTRSTMLDILRQDFVRTARAKGLVERGVVLRHALRNALIPTVTVVGVQA
                     GYLLGGAILTETVFAWPGVGTLMIQGILARDFPLVQGCVLVIALGFVLFNLAVDLLYA
                     FLDPRIRYE"
BASE COUNT          484 a         1116 c         1133 g          395 t
ORIGIN      
        1 catgacccgc ccgctgatcg ctttcgcaac cttgctcctc tcgctggcgc tgccggtcgg
       61 catcgcgcac gcgggtgagg actggccggt cgatcccggc gtcgcggagc gccccccgga
      121 tctcccgtcc tggctgatcg cctcccggct cgggcggccg ctcgacgaca gatcccgtga
      181 tgatgccatg ggcagcagcg gcctcagtga cctgggcgcg gtgctgctgc gccagggtcc
      241 cggcgccccg atcaattccg atcccctgac gctccggacg tccggcgagg actcgaccga
      301 tctgcgcgcg ctcctcgggc gtggcgatcg ctggcacctg atgagcttcg gcgccgcggc
      361 cgccaaccgg ccacaagata tgaaggtgac cgcgtacggg gccgtggcct atctcccgag
      421 cctgtcgagc tccggtcccg acgggagtct cggcttcggg ccggccaccg cgcccggcgc
      481 ggccttcggc ctcggcctgg acgatccgga ggagccggtg tcgctcggct tcaagggcga
      541 gctcgccggc ctcgggctcg agggcggcgc ggagtaccgc tcggtcggca agcgactgga
      601 gcgggtcgtg accgggcccg cttcccagaa ggaccgggag ggtaccgagg tctggctggc
      661 ccagacccta ggcctcctgc ggctcaagct gtcgcagtcc gatctctccg acaacgtgga
      721 ccgggatccc gcggtgcccc ggaccaccaa ggtccagacc gcggtcagcg cgcagctcgc
      781 cccgcgcgac tggccgatct tcggcctcac ctatgccacc ggggactccg agcgcacctg
      841 gctcaccggc gagggccgca cccgcacggt cgagcggcag accttcgaca gcgtggccgg
      901 gtcggcgtac tacggggcgc ggtggttcga tctctcgggg acctccacct acagctacag
      961 tcgagagccc ggccgatccg atcgcgacat gaactcgctt taccacgacc tgagcctcac
     1021 cctgcgcccg gtcaacgtcc tgtccgtcac cccgtctctc agcagcgggg tggaccggta
     1081 cgagtggtcg tcgacccgct accagttcgg ctcggcgtcg ctcctgctca cctacacgcc
     1141 gcccgcgagc cgcttcagcc tgtggacgct cggggcctac accacgtccc agagcacgga
     1201 ccggtcggtg gacgggcgca ccatgagcgt gagcggaggc ctggcgtgcg ggctcggccg
     1261 gatcctcggg ggacaggcga gcgtcgcggt ccaggccggc tacgatcgct acgtggacgg
     1321 gatctacccc gacagctcga cgcgcggagc cttcgggctg gtcctcctga aagtcgccgc
     1381 cttctagccg ttccccacct ccgcgggcgg cggcgggtca gccgtgccgg agccgcggat
     1441 ccaggaagtc ctgcagtgcg tcgccgagga tattgagcga cagcaccacc acgaagatcg
     1501 ccagcccggg cagcagcgcc acgtgcgggc gcaggatcac gaaggtgcgc gcggtggcca
     1561 gcatgctgcc ccagtccgcg gtgggcggct gcgtgcccag gccgaggaag ctgagcccgg
     1621 cggtggcgag gatcttggcc ccgacgtcga ggctccacgc caccaccacc ggcgccagca
     1681 ggtggggcag caggtggcgg cgcaggatcg accggtcgct gctgccgagg gcgcgcgccg
     1741 cctccacgaa ctcacgctcc cgcagggaga ggaccaagcc ccgcgccagg cgcgtgtaga
     1801 gggggaagcc cacaattgcg atcgccacca tcgcgttggc gaggccgggg ccgaggccgg
     1861 ccacgatggc gatggcgagc aggatgtagg ggaacgccat caggatgtcg gtcaggcgca
     1921 tcaccaccgt gtccacccgc ccgccccagt agccggcgtg caccccgagg agcccgccga
     1981 ccagcatcga gacgctcgcg gtggcgaacc cggccagcag cgagatccgt ccgccccaca
     2041 ccaggcgggc cagcatgtcg cgcccgaact cgtcggtacc gagcaggtgg ccggggctcc
     2101 ccggcgggcg cagccggttg cgggtgtcga ccgcgtcggg atcgagcagc ggcaggtagg
     2161 gcgcgagcag cgaggcgagc accgccgcgg ccatgaccat gcccgcggcg agggcgagct
     2221 ggttgcgcgg gatacggtgc agcacgcgag ggagcctcat tcgtggaagc tcattcgtac
     2281 cggatgcgcg ggtccaggaa ggcgtagagc aggtcgaccg cgagattgaa gagcacgaag
     2341 ccgagcgcga tcaccagcac gcagccctgc acgagcggga agtcgcgcgc gaggatgccc
     2401 tggatcatca gggtgcccac ccccggccac gcgaacacgg tctcggtgag gatcgcgccg
     2461 cccagcaggt agccggcctg cacgcccacc accgtcaccg tcgggatcag cgcgttgcgc
     2521 agcgcgtggc gcagcacgac gccgcgctcc accaggccct tggcgcgagc ggtgcgcacg
     2581 aagtcctggc gcaggatgtc gagcatcgtg ctgcgggtga gccgcgcgat gatggtcgtg
     2641 gacgcggcgg ccagggtgac cgcgggcagg gcgaggtggc tcagcagatc ggcgagaccg
     2701 ccggtgccgt gggcgccgta catcccggag gcgggaagcc agccgagcca gagcgagaag
     2761 cacaccatga ggacgagccc cagccagaag ctcggcatgc tcgcgccgaa gatggagccg
     2821 agcgtgctga gccggtccac gagcgaccgc gggcgcgccg ccgagagcac tccgagcgcg
     2881 atgccgcata cggtggagag gagcaaggcg gtggtggtga ggaggagggt ggcccggaag
     2941 cggatcatca cctcgccgag caccggctgg cgcagccgga tggaccggcc gagatcaccc
     3001 tgcagcacgt gggccatcca gcgcgcgtac tgcagcggca agggctcgtc gagccccagg
     3061 tcggcccgca ggcgcgccac gtcctcctgg ctcgcctgcg accccagcat gatctcggcg
     3121 ggatcccc
//
LOCUS       DATMCV010000372         2315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_18481, whole genome shotgun sequence.
ACCESSION   DATMCV010000372 DATMCV010000000
VERSION     DATMCV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18481"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VNU03_08670"
     CDS             <1..1324
                     /locus_tag="VNU03_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP74575.1"
                     /translation="IIGGRIEVTALRADGTEFPVELTVTAPRVGGPPAFGAYVRDLTE
                     QRRADTARASLEMQLQQSQKMEAIGRLAGGVAHDFNNLLTVITGRAHMLLGRLKAGEP
                     MHRDVELIQKTSQRAVALTSQLLAFSRKQVVQPRVVELGPLVAELVPMLQRMIREDVE
                     LAVEPVEGSGRVKVDPSQMQQVLMNLAVNARDAMPGGGRIGVAIRDVEVSEAAAASRP
                     NLPPGPYVTLAVSDAGTGMSAETAAHIFEPFFTTKEQGKGTGLGLSTVYGIVEQSRGH
                     IEVQSELGRGTTFTIYLPRVDEPLVETPVDTGRRLRTTARTVLVVDDEPEVLELATEI
                     LRRVGYTVLAATDGANALEVAGRHEGEIHLLVTDMVMPGMSGRDLAERLSALRASLRV
                     LYISGYVQDASARAALASETSAFVAKPFTPELLTDRVRELLATAEAEA"
     gene            complement(1456..>2315)
                     /locus_tag="VNU03_08675"
     CDS             complement(1456..>2315)
                     /locus_tag="VNU03_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWP74576.1"
                     /translation="SVGGDLFTLSLPSDVEPMPEGLPLIHLDLDPWEIGKNYPAQVAI
                     QGDPKATLPELVEALRRRLSPAQAKEVGARVERLRIAQEARREALRQEAASQASRSPI
                     SPLALVGAVAGAVPDEAIIVDESISSGHGVRELLRARDHRGFYGLRGGGIGWGLPASL
                     GVKLAQPDRPVVALVGDGSAMYTIQSLWSAAHDSIAVVFVIFNNQSYRILKQRTQALK
                     GFSAEDNHYVAMDLVKPGLDYVGLARAQGVPGEIVEKTADVATALRRGLASGGPYLLD
                     VRIDGNLRG"
BASE COUNT          335 a          842 c          844 g          294 t
ORIGIN      
        1 gatcatcggg ggccgcatcg aggtgactgc cctgcgcgcc gacggcaccg agttcccggt
       61 ggagctgacc gtgaccgccc cgcgggtggg cggcccgccc gcgttcggcg cgtacgtccg
      121 tgacctcacc gagcagcggc gggccgacac cgcgcgcgcg agcctggaga tgcagctcca
      181 gcagtcccag aagatggagg cgatcggccg gctcgcgggc ggagtcgccc acgacttcaa
      241 caacctgctc accgtgatca ccggccgggc gcacatgctg ctgggccggc tcaaggccgg
      301 cgagccgatg caccgcgacg tcgagctgat ccagaagacg tcgcagcgcg cggtggcgct
      361 cacgagccag ctcctggcct tcagccgcaa gcaggtcgtg cagccgcggg tggtcgagct
      421 ggggcccctc gtggcggagc tggtcccgat gctccagcgc atgatccggg aggacgtcga
      481 gctggcggtc gagccggtcg agggctcggg gcgggtgaag gtcgatccga gccagatgca
      541 gcaggtcctc atgaacctcg cggtgaacgc gcgcgacgcc atgccgggcg gcgggcgcat
      601 cggggtcgcc attcgcgacg tcgaggtcag cgaggcggcc gcggcgagcc ggcccaacct
      661 cccgccgggg ccctacgtga ccctcgcggt gagcgacgcc ggcaccggca tgagcgcgga
      721 gaccgccgcc cacatcttcg agccgttctt caccaccaag gagcagggga agggaaccgg
      781 gctcggcctc tccaccgtgt acggcatcgt ggagcagagc cgcggccaca tcgaggtgca
      841 gagcgagctc gggcgcggca ccaccttcac gatctacctg ccccgggtgg acgagccgct
      901 ggtcgagacc ccggtcgata ccggccgccg gctacgcacc accgcccgga cggtgctggt
      961 ggtggacgac gagccggaag tgctcgagct ggccaccgag atcctccgcc gcgtcggcta
     1021 cacggtgctg gccgcgaccg acggggcgaa cgcactcgag gtggccgggc ggcacgaggg
     1081 ggagatccac ctgctggtga ccgacatggt gatgccgggc atgtcgggcc gcgacctggc
     1141 cgagcgcctg agcgcgctcc gcgcctcgct gcgcgtgctc tacatctcgg gctacgtgca
     1201 ggacgcctcc gcccgcgccg cgctcgcgag cgagacgtcc gcgttcgtgg ccaagccgtt
     1261 cacccccgag ctgctgaccg accgcgtgcg cgagctcctc gccaccgccg aagccgaggc
     1321 ctagcccgag ccggttggcg agaaggggtc agcggcgagg cggcgcccga aggtcgcacg
     1381 cgaggcgtac tccccgtacg tcgagcgtgc ggccgagggc gccaacgaag ccgatggccc
     1441 cttctcgccg accggctagc ctcgcaggtt gccgtcgatg cggacgtcca gcagataggg
     1501 gccgccggag gcgagacctc ggcggagtgc cgtcgcgacg tcggccgtct tctccacgat
     1561 ctcgcccggc acgccctgcg cccgggcgag gcccacgtag tcgaggcccg gcttcaccag
     1621 gtccatcgcc acgtagtggt tgtcctcggc cgagaagccc ttcagcgcct gggtccgctg
     1681 cttgaggatg cggtaggact ggttgttgaa gatcacgaag accaccgcga tggagtcgtg
     1741 cgcggcggac cagagggact ggatggtgta catcgcgctg ccgtcgccga ccagcgcgac
     1801 gaccggccgg tcgggctggg ccagcttcac cccgagggag gcgggcaggc cccagccgat
     1861 gccgccgccg cgcaggccgt agaagccgcg gtgatcgcgc gcgcgcagca gctcgcgcac
     1921 cccgtggccc gaggagatcg actcgtcgac gatgatcgcc tcgtcgggca ccgcgcccgc
     1981 caccgccccc accagcgcga gcgggctgat cggggagcgg ctcgcctgcg acgccgcctc
     2041 ctggcgcagc gcctcgcggc gcgcctcctg cgcgatgcgc agccgctcca cccgcgcgcc
     2101 cacctccttg gcctgggccg gactcaggcg ccgccgcagc gcctccacca gctcgggcag
     2161 cgtcgccttc gggtcgccct ggatcgccac ctgcgccggg tagttcttgc cgatctccca
     2221 cgggtcgaga tcgaggtgga tgagcggcag gccttccggc atcggctcca cgtcggacgg
     2281 cagcgagagg gtgaagaggt cgccgcccac cgaga
//
LOCUS       DATMCV010000373         3126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_353907, whole genome shotgun sequence.
ACCESSION   DATMCV010000373 DATMCV010000000
VERSION     DATMCV010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353907"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VNU03_08680"
     CDS             complement(<1..219)
                     /locus_tag="VNU03_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74577.1"
                     /translation="MITATVRYKLPPHIDYAACRAHFHKIAPGFQDVKGLISKHFIWS
                     ENGWAGGVYQWETREDAQSFYGGAWLDGI"
     gene            complement(239..1195)
                     /locus_tag="VNU03_08685"
     CDS             complement(239..1195)
                     /locus_tag="VNU03_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWP74578.1"
                     /translation="MTHKVLLTTPRFFPFEAMIREHLAEHGCEVLEHCAHDMQMKEET
                     LLTLVPEAAGLITALEPVTARVLAAAPRLRVICATGVGYDHIDVEAATARGVTVCISA
                     GSNHQAVAELTLGLMVAVARQIAYGDRLLRGGGWERLVGPELWGKTLGIVGLGRVGKS
                     VALLARAFGMRVLATDVVLDHTFGSAHAVEYVPLAKLLRRADFVSLHCPLTPGTRGLM
                     NERTFGLMKPGVYLINTARGPVVEEDALVQALRSRQIAGAALDVFPDEPHIGAGLREA
                     PNVVLTPHMGGSANEALERMMELALQNVTQVLGGGAPVGRVN"
     gene            complement(1192..2085)
                     /gene="dapA"
                     /locus_tag="VNU03_08690"
     CDS             complement(1192..2085)
                     /gene="dapA"
                     /locus_tag="VNU03_08690"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWP74579.1"
                     /translation="MKLTKDQLIGLYPALPTPTDPSGTVDRKATARLVEFLCAAGVNG
                     LVPVGGTGEYAALLPAERRTMVEVTVDAAAGRVPVVAGILAPGFKESVQAAKDFVVAG
                     AVGVMLVTPYYVTPTQEGIREYFRAFAQEVPVPVMLYEIPYRTGISMKAETIAQMADD
                     GSIIGMKACNPDFNQFARVIALAGDRISVMSGEEPFFATHVAMGARGGVLATANVFPK
                     IWQEIFALASAGNLKGALARQQQIVPLLDAAFSETNPGPLKELMAMMGQPVGHVLRPL
                     QRPRPETMEKLQAAVRVLQAT"
     gene            complement(2110..>3126)
                     /locus_tag="VNU03_08695"
     CDS             complement(2110..>3126)
                     /locus_tag="VNU03_08695"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74580.1"
                     /translation="LLLVAALVVFYLYDRALGMSTLASGTPALDRRGDGRENLISRLS
                     AWAGGRLIAILGWLCDRAAEVTERLVPARPDRPARPWARGVLVIASLLILAFLAVPAF
                     FMVPVSFTTSGFIDWPPRGFSLKWYRAYLESPQWMAATVRSVGVGVVSATLAMLIGVP
                     AAFALARREFAGKTGALALILSPLVIPRMIIAVALFYLYARIGLVGTSLGLVLGHAVL
                     AIPFVVVTVVAVLKNYDERLDQAASSLGANRARTLYHVTLPLIRGGLVAAFLFAFVTS
                     FDELTIALFVTGGLTTTLPKQMWDDALLKVSPVLAAVSTVLIVFVAVTISLAERLRQG
                     AARA"
BASE COUNT          493 a         1138 c         1081 g          414 t
ORIGIN      
        1 gatgccgtcg agccacgccc cgccgtaaaa ggactgggcg tcctcgcgag tctcccactg
       61 ataaacgccg cccgcccagc cgttctcgct ccagatgaag tgcttgctga tcagtccctt
      121 cacgtcctgg aatccgggcg cgatcttgtg gaagtgcgcg cggcaggcag cgtagtcgat
      181 gtggggcggg agcttgtagc ggaccgtggc ggtgatcata tggactcctc gcgcccggct
      241 agttcacccg gccgaccggc gcgccgcccc cgagcacctg cgtcacgttc tggagggcga
      301 gctccatcat ccgctcgagg gcctcgttcg cgctgccgcc catgtgaggg gtcaggacga
      361 cgttcggggc ctcgcgtagc ccggcgccga tgtggggctc gtccggaaac acgtcgagcg
      421 cggcccccgc gatctgccgc gagcggaggg cctggaccag ggcatcctcc tccaccaccg
      481 ggccgcgggc ggtgttgatc aggtagacgc ccggcttcat caggccgaag gttcgctcgt
      541 tcatcagccc gcgcgtcccc ggcgtgagcg gacagtggag cgagacgaag tcggcgcggc
      601 gcagcagctt cgccagcggc acgtactcga cggcgtgcgc gctcccgaac gtgtggtcga
      661 gcacgacgtc ggtcgcgagg acccgcatgc cgaacgcgcg cgcgagcagc gccacgctct
      721 tgcccacccg ccccagcccg acgatgccca gcgtcttgcc ccacagctcg ggcccgacga
      781 ggcgctccca ccccccgccc cgcagcaggc ggtcgccgta cgcgatctgc cgcgccaccg
      841 ccaccatgag ccctaacgtg agctcggcga ccgcctggtg gttgctcccc gccgagatgc
      901 agaccgtcac cccccgcgcg gtcgccgcct ccacgtcgat gtggtcgtac ccgacgccgg
      961 tcgcgcagat cacccgcagg cgcggcgccg cggcgagcac ccgggccgtc accggctcga
     1021 gcgcggtgat cagccccgcg gcctccggca ccagcgtgag cagcgtctcc tccttcatct
     1081 gcatgtcgtg cgcgcaatgc tcgagcacct cgcacccgtg ctccgccaga tgctcgcgga
     1141 tcatcgcctc gaaggggaag aaccgcggcg tggtgagcag gaccttgtgc gtcatgtcgc
     1201 ctgcagcacc cgcacggccg cctgcagctt ctccatcgtc tcgggccggg gccgctggag
     1261 gggacgcagc acgtggccga ccggctggcc catcatggcc atgagctcct tgagcggccc
     1321 ggggttcgtc tccgagaacg ccgcgtccag gagcgggacg atctgctgct ggcgggccag
     1381 cgcccccttc aggttgccgg cgctcgccag cgcgaagatc tcctgccaga tcttcgggaa
     1441 cacgttcgcg gtcgcgagca cgccgccgcg cgcgcccatg gccacgtggg tcgcgaagaa
     1501 cggctcctcg ccgctcatga cgctgatacg gtcgcccgcc agcgcgatca cccgcgcgaa
     1561 ctggttgaag tcggggttgc acgccttcat cccgatgatc gagccgtcgt cggccatctg
     1621 cgcgatggtc tcggccttca tcgagatgcc ggtgcggtac gggatctcgt agagcatcac
     1681 cggcaccggc acctcctggg cgaacgcccg gaagtactcg cggatcccct cctgggtcgg
     1741 ggtcacgtag tacggcgtga ccagcatgac gccgacggcg cccgccacca cgaagtcctt
     1801 ggcggcctgg acggactcct tgaagccggg cgcgaggatg cccgccacca cgggcacgcg
     1861 gccggccgcc gcgtccaccg tcacctccac catcgtgcgg cgctccgccg ggaggagcgc
     1921 ggcgtactcc ccggtgccgc ccacgggcac cagcccgttg acgccggcgg cgcagaggaa
     1981 ctcgacgagc cgcgcggtcg ccttgcggtc cacggtgccg ctcgggtcgg tgggggtcgg
     2041 cagagccgga tagaggccga tgagctggtc cttggtcaat ttcatgcggt gttcctcctc
     2101 atgccagggt caggctcgag cggcgccctg gcggagccgc tcggccagcg agatggtaac
     2161 agccacgaac acgatgagca cggtcgacac cgcggcgagg accgggctga ccttcagcag
     2221 ggcgtcgtcc cacatctgct tcggcaaggt cgtggtgagg ccgccggtga cgaagagcgc
     2281 gatcgtcagc tcgtcgaacg aggtgacgaa cgcgaacagg aacgcggcca cgagcccgcc
     2341 gcggatgagc gggagggtca cgtggtacag ggtgcgggcc cggttcgccc cgaggctcga
     2401 ggcggcctgg tcgagccgct cgtcgtagtt cttgagcacc gccaccaccg tcacgaccac
     2461 gaacgggatc gccaggaccg cgtgccccag gacgaggccg agcgaggtcc ccaccagacc
     2521 gatgcgcgcg tacaggtaga agagggccac cgcgatgatc atacgcggga tgaccagcgg
     2581 cgacaggatg agcgcgaggg cgcccgtctt ccccgcgaac tcgcgccggg ccagcgcgaa
     2641 cgcggcgggc acgccgatga gcatcgccag ggtcgccgac acgaccccca cccccaccga
     2701 tcggacggtc gcggccatcc actgcggcga ctcgagatag gcgcggtacc acttcaacga
     2761 gaagccccgc ggcggccagt cgatgaaccc ggacgtcgtg aacgagaccg gcaccatgaa
     2821 gaacgcgggc accgcgagaa aggccaggat cagcagggag gcgatgacga ggacgccccg
     2881 cgcccacggg cgggccggcc gatcgggccg cgccgggacc aggcgctcgg tcacctcggc
     2941 cgcgcgatcg cagagccatc cgaggatcgc gatcagccgg cctcccgccc acgccgagag
     3001 ccgcgagatc agattctccc ggccgtcgcc gcgccggtcg agcgccggcg tcccgctggc
     3061 gagcgtcgac atgccaaggg cgcggtcgta caggtagaag acgacgagcg cggcgaccag
     3121 caggag
//
LOCUS       DATMCV010000374         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_88768, whole genome shotgun sequence.
ACCESSION   DATMCV010000374 DATMCV010000000
VERSION     DATMCV010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88768"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VNU03_08700"
     CDS             <1..537
                     /locus_tag="VNU03_08700"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74581.1"
                     /translation="RAEDGAALMARALAAGHEGLMAKDPASPYEPGGRGKKWLKLKAA
                     LTVDCVIVAADRGSGRRVGWLSNYHLAVRDGDGWAEVGKTFKGLTDREFTEMTERLRA
                     LATADDGYTVRVRPEVVVEVDYNEIQKSPTYPSGFALRFARIARVRPDKAPGQATTLE
                     ELRSLYERQFATKGRAEP"
     gene            complement(534..1181)
                     /locus_tag="VNU03_08705"
     CDS             complement(534..1181)
                     /locus_tag="VNU03_08705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP74582.1"
                     /translation="MTQEMTAYYARRAAEYERVYTGPRWQDDLARLRPRVTELFAGRR
                     VFEVACGTGYWTHVAAERARAVHATDLNDDTLAIARAKSYTAPVSFERRDAYAPAAGP
                     ARFDGGLAGLWLSHVDLARMDEFLRAFHSHLEPGARVLMFDERATEERPNPPVSRFDE
                     AGNRYEMRRLQDGERFEIVKNLFDRARLEALVAPHAASISYRELQFFWLLEYAVA"
     gene            complement(1192..2229)
                     /locus_tag="VNU03_08710"
     CDS             complement(1192..2229)
                     /locus_tag="VNU03_08710"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWP74583.1"
                     /translation="MPPSTMLDTTIAGSLPKPEWLAPPNVLWAPWRLEGRPLEEGKRD
                     AVLLALREQEQAGIDIVTDGEQTRRHFVWGFVEQLDGIDFSKMVTIGIRADRYQATVP
                     TVTGPVRRRGPIHGDDVRWARANTGQRLKLTIPGPMTIVDTIHDGHYGSRARLAMDLA
                     RLINEEARELEALGLDMLQLDEPAFNVYMDEVRDWGVAALDRAVEGLRCRTAVHICYG
                     YGIKANTDWKQTLGGEWRQYEQTFPLLAGSRIGGVSLECAGSRVPISLIGLLGDKDVL
                     VGAVDVATDRVETPEEVAAVIRSAMQHVPAARIFPCTNCGMVPLSRGVARDKLRALGA
                     GAAIVRRELAG"
     gene            2236..2505
                     /locus_tag="VNU03_08715"
     CDS             2236..2505
                     /locus_tag="VNU03_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74584.1"
                     /translation="MTAVIRRWCLLIVVATGLLIGCSSERDRQWYKPNVNYTVDEFKR
                     DRDGCTSKDNVLDEQCMKERGWVPLTGDRAAPPKPSSTNPRGPRY"
     gene            2489..>2975
                     /locus_tag="VNU03_08720"
     CDS             2489..>2975
                     /locus_tag="VNU03_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP74585.1"
                     /translation="MGLATSLRAIVFDFGGVLWDMRWDVARELDRAHDLPRSSIFQTL
                     YGSPVWAEIECGVGDPVAWTDGAHRELERRAGRTLPRLHDEWRKAQAPIELNVALVRA
                     LRPPYRCSILSNADVSLRGRLEGELALHHLFDDIVVSAEVGMAKPTPAIFRLAAERLG
                     LP"
BASE COUNT          411 a         1051 c         1099 g          414 t
ORIGIN      
        1 cgggcggagg acggcgccgc gctcatggcc cgcgccctcg cggccggcca cgagggcctg
       61 atggccaagg acccggcgag cccctacgag ccgggcggcc gcggcaagaa gtggctcaag
      121 ctgaaggccg cgctcacggt ggactgcgtg atcgtggccg cggaccgcgg ctccggccgg
      181 cgcgtgggct ggctctccaa ctaccacctg gcggtgcgcg acggggacgg ctgggcggag
      241 gtgggcaaga ccttcaaggg gctcaccgat cgcgagttca ccgagatgac cgagcggctg
      301 cgcgcgctgg ccaccgccga cgacggctac acggtgcggg tgcggccgga ggtcgtggtg
      361 gaggtggact acaacgagat ccagaagagc ccgacctacc cgtccggctt cgcgctgcgc
      421 ttcgcccgca tcgcgcgcgt ccgcccggac aaggccccgg gccaggccac cacgctcgag
      481 gagctgcgct cgctctacga gcgccagttc gcgacgaagg gccgggccga gccctaggcc
      541 accgcgtact cgagcagcca gaagaactgc agctcccggt acgagatgga cgccgcgtgc
      601 ggggcgacca gcgcctccag gcgcgcgcga tcgaagaggt tcttgacgat ctcgaagcgc
      661 tcgccgtcct ggaggcgccg catctcgtag cggttacccg cctcgtcgaa gcggctgacc
      721 ggcgggttcg ggcgctcctc ggtggcgcgc tcgtcgaaca tcagcacgcg ggcgcccggc
      781 tccaggtggg agtggaacgc gcggaggaac tcgtccatcc gcgcgagatc cacgtgcgac
      841 agccacaggc cggcgaggcc gccgtcgaag cgcgcgggtc ccgctgcggg cgcgtaggcg
      901 tcgcgacgct cgaacgagac cggggccgtg tacgacttgg cccgggcgat cgcgagagtg
      961 tcgtcgttga ggtcggtggc gtggacggcg cgggcgcgct ccgccgcgac gtgggtccag
     1021 tagccggtgc cgcaggcgac ctcgaacacc cggcgccccg cgaagagctc cgtcacacgc
     1081 gggcgcagcc gggccaggtc gtcctgccag cgcgggccgg tgtagacccg ctcgtactcc
     1141 gccgcccgtc gggcgtagta cgcggtcatc tcctgcgtca tcggctgggg atcagccggc
     1201 cagctcgcgc cgcacgatgg cggcccccgc gccgagcgcc cgcagcttgt cgcgcgccac
     1261 gccgcgcgag agcgggacca tcccgcagtt ggtgcagggg aagatgcggg ccgcgggcac
     1321 gtgctgcatc gcggagcgga tcaccgcggc gacctcctcc ggcgtctcca cccgatcggt
     1381 ggccacgtcg accgcgccga ccagcacgtc cttgtcgccg agcaggccga tgagggagat
     1441 cggcacgcgc gagcccgcgc actcgaggga cacgccgccg atgcggctgc cggccaggag
     1501 cgggaaggtc tgctcgtact gccgccactc gccgcccagg gtctgcttcc agtcggtgtt
     1561 ggccttgatg ccgtagccgt agcagatgtg caccgcggtg cggcagcgga ggccctcgac
     1621 cgcgcggtcg agcgcggcga cgccccagtc gcgcacctcg tccatgtaca cgttgaaggc
     1681 gggctcgtcg agctgcagca tgtcgagccc gagggcctcc agctcgcgcg cctcctcgtt
     1741 gatgaggcgg gcgaggtcca tcgcgagccg ggcccggctg ccgtagtgcc cgtcgtggat
     1801 ggtatcgacg atggtcatcg gcccggggat cgtcagcttg agccgttggc cggtgttggc
     1861 ccgggcccag cgtacgtcgt cgccgtggat cgggcctcgg cggcgcaccg gcccggtcac
     1921 ggtcggcacc gtcgcctggt agcggtccgc ccggatgccg atcgtcacca tcttcgagaa
     1981 gtcgatgccg tcgagctgct cgacgaagcc ccagacgaag tgccggcggg tctgctcgcc
     2041 gtcggtgacg atgtcgatgc cggcctgctc ctgctcgcgc agggccagga gcaccgcgtc
     2101 gcgcttgccc tcctcgagcg ggcggccctc gaggcgccac ggcgcccaca gcacgttggg
     2161 gggcgcgagc cactcgggct tggggaggct gccggcgatg gtggtgtcca gcatggtcga
     2221 gggcggcatg gtatcatgac cgccgtgatc cggcgttggt gcctcctcat cgtcgtcgca
     2281 acgggtctcc tgatcggctg cagctccgag cgggatcggc agtggtacaa gccgaacgtc
     2341 aactacacgg tggatgagtt caagcgcgac cgcgacggct gcaccagcaa ggacaacgtc
     2401 ctcgacgagc agtgcatgaa ggagcgcggc tgggtgccgc tcaccggcga ccgcgccgcg
     2461 ccgcccaagc cgtcgtcgac gaatccgcgt gggcctcgct actagcctcc gcgcgatcgt
     2521 cttcgacttc ggcggggtcc tgtgggacat gcgctgggac gtggcccggg agctggaccg
     2581 agctcacgac ctgccgcgct cctcgatctt ccagaccctc tatggctcgc cggtctgggc
     2641 cgagatcgag tgcggagtgg gcgacccggt cgcgtggacc gacggcgcgc accgggagct
     2701 ggagcgccgg gcggggcgga ccctgccgcg cctgcacgat gagtggcgca aggcgcaggc
     2761 gcccatcgag ctcaacgtcg ccctggtgcg ggcgctgcgc ccgccctacc gctgctcgat
     2821 cctgagcaac gcagacgtgt ccctgcgtgg gcgtctcgaa ggcgagctgg cgctgcatca
     2881 tctcttcgac gacatcgtgg tctccgccga ggtcggcatg gcgaagccga cgcccgcgat
     2941 cttccggctc gcggccgagc gcctcggcct gcccc
//
LOCUS       DATMCV010000375         2005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_88805, whole genome shotgun sequence.
ACCESSION   DATMCV010000375 DATMCV010000000
VERSION     DATMCV010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88805"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VNU03_08725"
     CDS             <1..1195
                     /locus_tag="VNU03_08725"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387596.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HWP74586.1"
                     /translation="DGGFKYNPPNGGPADTDVTGLVERRANQLLREGITEVKRVPVEA
                     AMKAATTHPEDLVTPYVADLRSVVDLDAVRAAGLRIAVDPLGGASRPYWEPINALYGL
                     DITVVNPVIDPTFAFMTVDHDGKIRMDCSSPYAMARLVGLRDRYRLAFANDPDADRHG
                     IVTPAAGLMNPSHYLAVAVDYLLSHRPRWSTAAAVGKTLVSSGLIDRVTAARGRRLFE
                     APVGLKWFVPGLLDGSLCFGGEESAGATFPRHDGRVWTTDKDGLILNLLAAEITARTG
                     RDPGERYLELTRELGTPWYTRIDAAATPAEKARLARLSPDSVKEAQLGGEPITDKLTR
                     APGNGAPIGGLKVVAASGWFAARPSGTEDIYKIYAESFRDQAHLDTIVGEAQAIVGNA
                     LRAPA"
     gene            1211..1816
                     /locus_tag="VNU03_08730"
     CDS             1211..1816
                     /locus_tag="VNU03_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP74587.1"
                     /translation="MDARDLFLEQHAAVHTAAVGGNKMSAAERTFAGLTDEQMRVRPR
                     EDLNSIAWLMWHIARAEDILVGQVVAGGPQLFDEGWKKRLNVIRPDFGIGMTSPEVTD
                     LTRQIDVGALREYRDAVGHRTRQIVSAFKPSDWDGAVQADAVERAAASGAFGPRTEAL
                     LKGFPGRPRTGLLSAIALLHSAAHMGEATTVRTSGGFGTGI"
     gene            complement(1827..1994)
                     /locus_tag="VNU03_08735"
     CDS             complement(1827..1994)
                     /locus_tag="VNU03_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74588.1"
                     /translation="MRIMEIDPDMVPWQGDLVTVKPDIADGHLRLPAGPGWGTEVDEA
                     AVRAHPPRAAR"
BASE COUNT          293 a          717 c          737 g          258 t
ORIGIN      
        1 ggacgggggc ttcaagtaca accctcccaa cggcgggccg gccgacaccg acgtgacggg
       61 cttggtggag cggcgcgcca accagctcct gcgcgagggg atcaccgagg tgaagcgggt
      121 gccggtcgag gccgcgatga aggcggcgac cacgcatccg gaagatctgg tgacgcccta
      181 cgtggccgat ctccggagcg tggtggacct ggacgcggtg cgggccgccg ggctgcggat
      241 cgcggtggac ccgctcggcg gcgcgtcccg gccctactgg gagccgatca acgcgctcta
      301 cgggctcgac atcacggtgg tgaacccggt catcgatccc accttcgcgt tcatgaccgt
      361 cgatcacgac ggcaagatcc gcatggactg ctcgagcccc tacgcgatgg cgcggctcgt
      421 cggcctccgg gaccggtacc ggctggcctt cgccaacgat cccgacgcgg accgccacgg
      481 catcgtcacg cccgcggcgg ggctgatgaa cccgagccac tacctggcgg tggcggtcga
      541 ctacctgctg agccatcggc cgcggtggtc gaccgccgcc gcggtgggca agaccctcgt
      601 ctccagcggc ctgatcgacc gggtgaccgc cgcgcgcggg cgccggctct tcgaggcgcc
      661 ggtggggctc aagtggttcg tgccgggcct gctcgacggc tcgctctgct tcgggggcga
      721 ggagagcgcg ggggcgacgt tcccgcgcca cgacggccgc gtctggacca ccgacaagga
      781 tggactgatc ctcaacctgc tcgcggccga gatcaccgcc cgcaccggcc gcgaccccgg
      841 cgagcgatac ctcgagctga ccagagagct cggcaccccg tggtacacgc gcatcgacgc
      901 ggccgcgacg cccgccgaga aggcgcggct cgcccggctc tcgccggact cggtgaagga
      961 ggcgcagctg ggcggcgagc cgatcaccga caagctcacc cgcgcccccg gcaacggggc
     1021 gccgatcggc gggctcaagg tggtggccgc gagcggctgg ttcgcggccc ggccgtccgg
     1081 caccgaggac atctacaaga tctacgcgga gagcttccgc gaccaggccc acctggacac
     1141 catcgttggc gaggcgcagg cgatcgtggg caacgccctg cgtgcgcccg cctgagaagg
     1201 agcgaacctc atggatgctc gtgacctgtt cctggagcag cacgccgccg tgcacaccgc
     1261 cgcggtcggc ggcaacaaga tgtcggccgc ggagcgcacc ttcgccggcc tcaccgacga
     1321 gcagatgcgg gtgcgcccgc gcgaggacct caactccatc gcctggctca tgtggcacat
     1381 cgcccgcgcg gaagacatcc tggtcggcca ggtcgtcgcc ggcggcccgc agctcttcga
     1441 cgaggggtgg aagaagcggc tcaacgtcat ccgccccgac ttcggcatcg gcatgacgag
     1501 cccggaggtg accgatctca cgcgacagat cgacgtgggg gcgctgcggg agtaccgcga
     1561 cgcggtgggg caccgcacgc ggcagatcgt ctccgccttc aagccgagcg actgggacgg
     1621 cgcggtgcag gccgacgcgg tcgagcgggc cgccgcgtcg ggcgccttcg ggccgcgaac
     1681 cgaggctctc ctcaagggtt tcccgggacg gccgcgcacc gggctgctga gcgcgatcgc
     1741 gctcttgcac tcggccgctc acatggggga ggccaccacc gtccgaacct cgggcggctt
     1801 cggcaccggg atctgagcgg gcgggcctag cgcgccgcgc ggggcgggtg cgcgcggacg
     1861 gctgcctcgt ccacctcggt gccccagccg gggccggcgg gcaggcggag gtggccgtcc
     1921 gcgatgtcgg gcttcacggt cacgaggtcg ccctgccacg gcaccatgtc ggggtcgatc
     1981 tccatgatgc gcaggttggg gacca
//
LOCUS       DATMCV010000376         4742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_156443, whole genome shotgun sequence.
ACCESSION   DATMCV010000376 DATMCV010000000
VERSION     DATMCV010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156443"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2720)
                     /locus_tag="VNU03_08740"
     CDS             complement(<1..2720)
                     /locus_tag="VNU03_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWP74589.1"
                     /translation="MASGADILVIDDDPIVRETVGDVLQEHGYHVDRVDRGEDALARI
                     GEPRPVDLAIVDCRLPDISGLELLQSIKTRSPETEVILITGHASLDGALAAMAGDASY
                     LVKPVDTAQLLSAVDRALARQGLLRALRGSEERYRLITESMTEALFLLDLDGALAMVN
                     GYGEQMTGFRSDELIGRPVFSVFDRAGALLVRDRLEAVRAGRKVAPQFECQVVRRDGR
                     RIPVEMTCTGIVRSGQVVGQLAVARDVTERKQSQAVQALLSSIVESSEDAIVGRTLEG
                     RIVSWNPAAERLYGYRAEEAIGRSISMIVPPERRDELPGILIRLHHGHRISNFETVCL
                     TKTGRRVDVSLTISPIRDAAGRVTGASSTARDISGRLRAERTARALAQVGHELLGTLD
                     LDQIAPRIVRAVLDVFRARRAVLYELEPASRTLVCVAAAGPADAAKWVGCHLPAGAGI
                     AWLAVNARRLVASEGALTDGPPVERAVEPFAEEMGQSVIAVPLMGQDRVLGALLVGSD
                     PERVYSGEEQHLLAVFGDQAALALRNAQLFTETERGRHIAERLAIVARSVPQSLTFTE
                     LGRQIADGVAGLLEARAAAVYRLDPETWDLVRVATSSVGGTALVADPVLSRGAGLEGI
                     AATTRQPAPTADLLEDPRVILSPAARQEVTASDHRAALALPLLVQGRVVGAVVARDRA
                     GRSWTGEDIALARAFADQAAVALENSHLYQGLQTALDDVRAGQHELIETERLRAIASL
                     TDGVTDYVRQVLQMIMGKVQVVLPQLEGTPGYDQLATIKTTVKEAAKVLKRLQSFAEV
                     RTLTEAPPLDFNDVVRAALQATRPCCTIGEGADARHVEVHVDHGELPAVAGEASVLIE
                     AVTVVLMNAIEALPDGGAITVKTWTDGSHVLCAIQDDGVGIAPDDQRRAL"
     gene            complement(2873..3265)
                     /locus_tag="VNU03_08745"
     CDS             complement(2873..3265)
                     /locus_tag="VNU03_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP74590.1"
                     /translation="MAESELHRKGNEIRRRLLGDKYVERVNATTYQDPIMRTFIDVAT
                     ENVFGALWARPGLDLKTRTLVCVVSDAATGREPELRIHLRMALGQGWTEQELVEVLIH
                     MSGYVGVPIIREAMICARDVFAEVRAER"
     gene            complement(3295..3624)
                     /locus_tag="VNU03_08750"
     CDS             complement(3295..3624)
                     /locus_tag="VNU03_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74591.1"
                     /translation="MGLGGAAPFTRVSGCDLCRAARITPWHHEDDICWIAECDVCDVP
                     MVVWRYHGTEPPAEHVTHMHTLLREVATRVLGGECYIDDNMRNIPDHYHAHGRPKGGF
                     FGPRSGR"
     gene            complement(3615..4010)
                     /locus_tag="VNU03_08755"
     CDS             complement(3615..4010)
                     /locus_tag="VNU03_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HWP74592.1"
                     /translation="MFRKPAAPPPERAAARRGPEARGDRSLTIPPSVAASLSRTVREQ
                     LAYLTPDQQRAFLWRYRAQAKSPLIALLFLPLGWHYLYLRKRGLQVLFWLTLGGLAAW
                     WVLDLFRIRRLVRDYNRRVAVHVMRALWG"
     gene            complement(4197..>4742)
                     /locus_tag="VNU03_08760"
     CDS             complement(4197..>4742)
                     /locus_tag="VNU03_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74593.1"
                     /translation="ALARYLVNEGARRRLAFAYLRDMQFDDGVSVPMHYLNPEGDIAL
                     VPVTMNCTVPPIPTPERAYEVGTALREMVHAYPGAERVAVLATGGLSHEPGGPRYFWV
                     DEEFDRWFLDLLRKGDHVALLRECTLERMEAAGSGGTAELLAWMVALAFTTGPAEVLA
                     YMPAIAWRSGTGMVVWPDVRN"
BASE COUNT          685 a         1695 c         1710 g          652 t
ORIGIN      
        1 tcgagcgcgc ggcgctggtc gtccggggcg atgcccaccc cgtcgtcctg gatggcgcag
       61 agcacgtgcg acccgtcggt ccaggtcttg accgtgatgg cgccgccgtc cgggagcgct
      121 tcgatcgcgt tcatgagcac caccgtgacc gcctcgatca gcaccgaagc ctcgccggcc
      181 accgcgggca gctcgccgtg gtcgacgtgc acctcgacgt gccgcgcgtc ggccccttcc
      241 ccgatcgtgc agcagggtcg ggtggcctga agcgccgccc gcaccacgtc gttgaagtcg
      301 agcggcggcg cctcggtcag ggtgcgtacc tccgcgaagg actgcagccg cttgagcacc
      361 ttggccgcct ccttcacggt ggtcttgatg gtggcgagct ggtcgtaacc cggggtgccc
      421 tcgagctgcg gcaggaccac ctgcaccttg cccatgatca tctggaggac ctggcggacg
      481 tagtcggtca ccccgtcggt cagcgaggcg atcgcgcgca gccgctccgt ctcgatcagc
      541 tcatgctgcc cggcccgcac gtcgtcgagg gcggtctgca gcccctggta gaggtggctg
      601 ttctcgaggg ccaccgcggc ctggtccgcg aacgcgcgcg cgagcgcgat gtcctcgccg
      661 gtccagctcc gtccggcccg gtcccgcgcc accaccgcgc cgaccacacg cccctgcacg
      721 agcagcggca gcgcgagcgc ggcgcgatgg tcgctcgcgg tcacttcctg ccgcgccgcc
      781 gggctcagga tcacgcgtgg gtcctcgagc aggtccgcgg tgggagccgg ctggcgggtg
      841 gtggccgcga tgccttcgag gccggctccg cgcgacagga ccgggtccgc gacgagcgct
      901 gtgcccccga ccgacgaggt cgccacccgc accaggtccc acgtctccgg gtcgagccgg
      961 tagaccgccg cggcgcgcgc ctcgagcagg ccggccaccc cgtcggcgat ctggcgtccc
     1021 agctcggtga aggtcagcga ctgcggcacg ctgcgggcga cgatcgcgag gcgctcggcg
     1081 atgtggcggc cccgctcggt ctcggtgaag agctgggcgt tgcgcagcgc cagcgcggcc
     1141 tggtcgccga agaccgcgag caggtgctgc tcctcgccgg agtacacgcg ctccggatcg
     1201 ctgcccacca ggagcgcgcc gagcacgcgg tcctgcccca tgaggggcac cgcgatcacg
     1261 gactgtccca tctcctccgc gaacggctcg acggcgcgct cgaccggggg gccatccgtc
     1321 agcgcgccct cggacgccac caggcgccgc gcgttcaccg cgagccaggc gatcccggcc
     1381 ccggccggga ggtggcagcc gacccacttg gccgcatcgg ccgggccggc ggcggccacg
     1441 cagacgagcg tgcgggaggc cggctcgagc tcgtacagca cggcgcgccg ggcccggaag
     1501 acgtcgagga ccgcgcgcac gatccggggc gcgatctggt cgaggtcgag cgtgccgagc
     1561 agctcgtggc cgacctgggc cagggcccgc gcggtgcgct cggcgcggag ccggccactg
     1621 atgtcgcgcg cggtggagga cgcgccggtg acgcgcccgg ccgcgtcccg gatcggcgag
     1681 atggtcagcg acacgtcgac gcggcggccc gtcttggtga ggcagaccgt ctcgaagttg
     1741 ctgatgcgat gtccatggtg cagccggatg aggatgccgg gcagctcgtc gcggcgctcc
     1801 ggcgggacga tcatcgagat cgaccggccg atggcctcct ccgcgcggta gccgtagagc
     1861 cgctccgcgg ccgggttcca gctcacgatc cggccctcga gcgtccggcc gacgatggcg
     1921 tcctccgacg actcgacgat cgaggacagc agcgcctgca ccgcctggga ttgcttccgc
     1981 tcggtgacgt cccgggccac cgcgagctgg cccaccacct ggcccgagcg cacgatgccg
     2041 gtacaggtca tctccaccgg gatgcgccgg ccgtcgcggc gcaccacctg gcactcgaac
     2101 tggggcgcga ccttgcgacc ggcccggacc gcttcgagac ggtcgcggac gagcagcgcg
     2161 ccggcccggt cgaacaccga gaagaccggc cgcccgatca gctcgtcgga gcggaagccg
     2221 gtcatctgct cgccgtagcc gttgaccatc gcgagcgcgc cgtccaggtc gagcaggaag
     2281 agcgcctcgg tcatgctctc ggtgatcagg cgatagcgct cctcggagcc ccgcagcgcg
     2341 cgcagcagac cctggcgggc gagggcgcga tccaccgcgg acaggagctg cgcggtgtcg
     2401 accggcttca cgaggtacga ggcgtccccc gccatcgcgg cgagcgcgcc gtcgagcgag
     2461 gcgtggccgg tgatcaggat gacttcggtc tcgggcgagc gggtcttgat gctctgcagc
     2521 agctcgaggc ccgaaatgtc cgggagccgg cagtcgacga tcgcgagatc taccggccgg
     2581 ggctcgccga tgcgcgcgag ggcatcctcg ccgcggtcga cccggtcgac gtggtagccg
     2641 tgctcctgca gcacgtcgcc cacggtctcc cggacgatcg ggtcatcgtc gatgacgagg
     2701 atgtcggcgc cggatgccat gggcggagga gtgtgcacgg gaagtgccac ccgttcggct
     2761 ccgacatgtg caacaaatca acaacttgga aataatcggt aatctttcca gatggttctg
     2821 gatcgacagg cgtgcccggc tgacactcgg acccgggccc cggagccccg ggtcaacgct
     2881 cggcgcgaac ctccgcgaac acgtcccggg cgcagatcat ggcctcgcgg atgatgggca
     2941 ccccgacgta gcccgacatg tggatcagca cctcgaccag ctcctgctcg gtccagccct
     3001 gcccgagcgc catgcgcagg tggatccgca gctcgggctc ccggccggtc gccgcgtcgg
     3061 acaccacgca caccagcgtc cgggtcttga ggtcgagacc gggccgggcc cagagcgcgc
     3121 cgaagacgtt ctcggtcgcc acgtcgatga aggtgcgcat gatcgggtcc tggtacgtcg
     3181 tcgcgttcac ccgctccacg tacttgtcgc ccagcagccg gcgccggatc tcgttgccct
     3241 tgcgatgcag ctcgctctcc gccatggaag cctcctgatg tgggggcgga cgcgctagcg
     3301 cccgctccgg ggaccgaaga acccgccctt gggccggccg tgggcgtggt agtggtcggg
     3361 gatgttgcgc atgttgtcgt cgatgtagca ctccccgccg aggacgcgcg tggccacctc
     3421 gcgcagcagg gtgtgcatgt gggtcacgtg ctcggcgggc ggctcggtgc cgtggtagcg
     3481 ccagaccacc atgggcacgt cgcagacgtc acactcggcg atccagcaga tgtcgtcctc
     3541 gtggtgccag ggcgtgatcc gggccgcccg gcacaggtcg catccgctga cgcgagtgaa
     3601 gggggcggcg cctcctagcc ccacagagcg cgcatgacgt ggaccgccac gcgacggttg
     3661 tagtcgcgga cgaggcggcg gatgcggaac aggtcgagca cccaccacgc ggcgagcccg
     3721 cccagcgtga gccagaacag cacctgcagg ccccgcttgc gcaggtagag gtagtgccac
     3781 ccgaggggca ggaacagcag cgcgatcagc ggcgacttcg cctgcgcgcg ataccgccag
     3841 aggaacgcgc gctgctggtc gggggtgaga tacgcgagct gctcgcgcac cgtgcgcgag
     3901 agcgacgcgg cgacggacgg aggaatcgtc aggctccgat cgccccgggc ctcggggccg
     3961 cgccgggccg ccgcgcgctc gggcggcggg gcggctggct tacggaacac cgatgattcc
     4021 cgctcagact gtgtccggct catgcccact ctcccactgg ggcgcgaggg agcaagaaca
     4081 gcgccagccg ggcgggtcag acggggcttg aacttagccg caggtcgctg atcggcgctt
     4141 ctcaagtgtc actccggtcg tccgcaggag gacggttgca agtcattgca gaggtgtcaa
     4201 ttcctgacat cgggccagac caccatgccg gttcccgagc gccaggcgat cgcgggcatg
     4261 tacgcgagca cctcggccgg tccggtcgtg aacgccagcg cgaccatcca cgcgagcagc
     4321 tccgcggtcc cgccggagcc ggccgcctcc atccgctcga gcgtgcactc gcgcaggagc
     4381 gcaacgtgat cgcccttgcg gaggagatcg aggaaccagc ggtcgaactc ctcgtcgacc
     4441 cagaagtagc gcggcccgcc cggctcgtgg gagagcccgc cggtcgcgag caccgcgacc
     4501 cgctcggcgc cgggatacgc gtgcaccatc tcgcgcagcg cggtcccgac ctcgtacgcg
     4561 cgctcgggcg tggggatcgg cggcaccgtg cagttcatgg tcaccggcac cagcgcgatg
     4621 tcgccctccg ggttcaggta gtgcatgggc accgagaccc cgtcgtcgaa ttgcatgtcg
     4681 cgcaggtagg cgaaggccag gcgccggcgc gcgccctcgt tgacgagata ccgggcgagc
     4741 gc
//
LOCUS       DATMCV010000377         3118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_156445, whole genome shotgun sequence.
ACCESSION   DATMCV010000377 DATMCV010000000
VERSION     DATMCV010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156445"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VNU03_08765"
     CDS             <1..184
                     /locus_tag="VNU03_08765"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWP74594.1"
                     /translation="QGIVQTSLETDVPVLSAVLTPHHFHEHAVHEEFFKQHMATKGKE
                     LAEACVAIIGQLAAVA"
     gene            complement(156..1550)
                     /locus_tag="VNU03_08770"
     CDS             complement(156..1550)
                     /locus_tag="VNU03_08770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HWP74595.1"
                     /translation="MPQINHRLIIRHPSKPAILIVSDAGRLRLPAFVSDDRHTAEVDH
                     INLTVQERFGLRTTVLRSLHHSDPLADVVVRIHELEAHGAGAPLPSGISWCGLDGLPS
                     GADGEDADVIAEWLAADAAPRAVVDGREWTLPGWFEEVCGWLEQALPDAGLREVREIV
                     QVRAWTSSCVLLVRTGAGTFYFKTVSESGRHECTVTEYLAQHFPETVARIVATKPERR
                     WFLMTALEGGKLEDVSDVMLWERAAAVYGQLQRACMERVRDLQTLGCPTRGLDTLAAE
                     IEPLSADVTALRPGEPDGLSAAEVDRLRAYGPELRRRCERLAACGIPLTLEHGDLWPG
                     NVLVDDATCAIIDWEDVAIGHPFISLAPLIVGLGMFQPRLSSPEVTQRLERAYLAPFA
                     GLAPPQRLREALRLAGPLSFVDMAVRYRGQRPSVVRLHPWMRDLVPHTLRLALAQLDT
                     EEGTRPTPPRRAGR"
     gene            1814..>3118
                     /locus_tag="VNU03_08775"
     CDS             1814..>3118
                     /locus_tag="VNU03_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWP74596.1"
                     /translation="MSIASQPVPAPSHPFADLDRLGDEIAELSAHLDAATARLLTLIR
                     EFDARGGWNSGFSSCAAWLGWRIGLDLGAARERVRVARALGTLPRLSEALACGELSYA
                     KVRALTRVATPATEARLLAVGRAGTAAHIERIVRGWRRVDRQAEARQATRQHADRGLH
                     VYPDDDGTVLVRGRLTPEVGSLLVKALDAARETLYQRARATAGAAPADPALAPPTRAQ
                     QQADALALLAETALLHEIDPGAPGERYQVVVHVDAAVLADPEQPGQSVLEEGSHVSAE
                     TSRRLACDASRVVMRHDDEGRLLEVGARTRTIPPALRRALLHRDRSCRFPGCHARVGE
                     GHHVRHWAQGGPTTLGNLVLLCRRHHHAVHDEGYRVFRGPDGALDFRRPDGRPLPEVP
                     PTDPVPADPIHTLHAQHAAQGLRLHARTACPGWLGERLDLGWA"
BASE COUNT          486 a         1110 c         1070 g          452 t
ORIGIN      
        1 gcagggcatc gtgcagacct cgctggagac cgacgtcccg gtgctctccg ccgtgctcac
       61 gccccatcac ttccacgagc acgcggtcca tgaggagttc ttcaagcagc acatggccac
      121 caagggcaag gagctggccg aggcctgcgt cgccatcatc ggccagctcg ccgcggtggc
      181 gtagggcgcg tcccctcctc ggtgtcgagc tgcgccaggg cgagccgtag agtgtgcggc
      241 acaagatcgc gcatccacgg atggagtcgc acgacggacg gtcgctggcc gcgatagcga
      301 accgccatgt cgacgaagct gagcgggccg gccaggcgca acgcctcgcg cagacgctga
      361 ggcggcgcca gacccgcaaa gggcgccagg taggcgcgct cgaggcgctg cgtcacctca
      421 ggcgaggaga ggcgtggctg aaacatgccc agccccacta tcagtggcgc caggctgatg
      481 aatggatgcc cgatggccac gtcctcccag tcgatgatgg cgcaggtcgc gtcatcgacc
      541 agaacgttac ccggccacag gtcgccatgc tcgagcgtca acggaatgcc acaggccgcc
      601 aggcgctcgc accttcgccg cagctccggc ccgtacgcgc gcagccggtc gacttcggcg
      661 gccgagagcc cgtccggctc gcccggccgc agcgccgtga cgtcggcgct gagcggctcg
      721 atctcggcgg cgagcgtgtc caggccgcga gttgggcagc caagcgtttg caggtcacgt
      781 acccgctcca tgcaggcgcg ctgcagctgt ccatacaccg ccgcggcccg ctcccagagc
      841 atcacatcgc tgacatcctc gagcttgccg ccttcgaggg ccgtcatgag gaaccagcgg
      901 cgctctggct tcgtggcaac gatccgcgcg accgtttccg gaaaatgctg ggcgaggtac
      961 tccgtcacag tgcattcgtg cctgccactc tctgacaccg tcttgaagta gaacgtaccg
     1021 gctccagtac gcacgaggag cacgcacgac gacgtccagg cgcgcacctg gacgatctcg
     1081 cgcacctctc gcagcccggc atctgggagc gcctgctcca gccagccgca aacctcttcg
     1141 aaccatccgg gcaacgtcca ttcgcggccg tccacgacag cacggggtgc cgcgtcggca
     1201 gcgagccact cggcaatgac gtccgcgtcc tcaccgtcgg cccccgaggg caagccatcc
     1261 agtccacacc agctgatgcc ggagggcagt ggcgccccgg cgccgtgcgc ctccagctcg
     1321 tggatccgaa ccaccacgtc ggcgagcgga tcggaatggt ggagactgcg cagaaccgtg
     1381 gtccgaagac cgaaacgctc ctggaccgtc agattgatgt ggtccacctc ggcagtgtgg
     1441 cggtcgtcgg atacgaacgc ggggaggcgc aggcggcccg catcgctgac gatgagaatc
     1501 gccggcttcg agggatgccg aatgatcagt cggtgattga tctgcggcac ggtctcggct
     1561 ctgctggttc cgggcgatcg tagcatgcct atgaaggtcg gttttctgct tgacaccctc
     1621 ggagcgaagc attgtcgacc gggcgggggc ctgcgaaaag aggcgtgctc agcgcaccac
     1681 gcgggaccgt gaagcgcgtt catcgccgcg atagtgaaaa gtccctcgtc cgcgtgcgct
     1741 cgcttgacgt aggcgctctc cggccttgcg aaatccgggg tgtagagtcc ttccacctgg
     1801 aggagggctc gccatgtcta tcgcctcgca acccgtgccc gccccgtcgc atccattcgc
     1861 agacctggac cgcctcgggg acgagatcgc cgagctgtcc gcgcacctcg atgccgcgac
     1921 tgcgcgcctg ctgaccctca tccgcgagtt cgacgcgcgt ggcggctgga acagcggctt
     1981 ctcctcctgc gctgcctggc tcggctggcg catcggcctc gacctcgggg ccgcccgcga
     2041 gcgggtgcgt gtcgcgcgcg ccctcggaac gctgccgcgc ctgtccgagg cgctggcctg
     2101 cggagagctc tcctacgcca aggtccgggc cctgacccgg gtggccacgc cagcgaccga
     2161 ggcgcggttg ctggcggtcg ggcgagcggg caccgcggct cacatcgagc gcatcgtgcg
     2221 gggctggcga cgtgtggacc ggcaggccga ggcccggcag gcgacgcggc agcacgcaga
     2281 ccgggggctg cacgtctacc cggacgacga tggcacggtg ctcgtccgcg gccggctgac
     2341 ccccgaggtc ggctccttgc tagtcaaagc gctcgacgcc gcccgcgaga cgctgtacca
     2401 gcgggcgcgc gccaccgccg gcgcggcacc ggcagatccc gccttggcgc ccccgactcg
     2461 cgcccagcag caggccgacg cgctggccct gctggccgag acggcgctac ttcacgagat
     2521 tgaccccggc gccccgggcg agcgctacca ggtagtggtc catgtggacg ccgcggtgct
     2581 agcggatccc gagcaacccg gccaatccgt gctggaagaa gggagccacg tttccgcgga
     2641 aacgtcgcgg cgcctggcct gcgacgcgag ccgggtggtc atgcggcacg acgacgaggg
     2701 gcgcttactc gaggtcgggg cccggacccg gacgattccg ccggcgcttc gacgcgcgct
     2761 gctccatcgg gaccggagct gtcgcttccc gggctgccat gcacgggtcg gagagggcca
     2821 tcacgtgcgt cactgggcgc agggcggccc aaccacgctc ggcaacctgg tactgctgtg
     2881 ccgtcgccat caccacgcgg tccatgacga gggctaccgg gtctttcgag gacccgacgg
     2941 cgcgctcgac ttccgtcggc cggatggccg gccgctaccg gaggtgccgc cgacagaccc
     3001 ggtgcccgcg gatccgattc atacgctgca cgcgcaacat gccgcgcagg gacttcggct
     3061 ccacgcgcga acggcgtgcc ccgggtggct aggtgagcgg ctcgacctcg gctgggcg
//
LOCUS       DATMCV010000378         3587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_620773, whole genome shotgun sequence.
ACCESSION   DATMCV010000378 DATMCV010000000
VERSION     DATMCV010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620773"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VNU03_08780"
     CDS             <1..648
                     /locus_tag="VNU03_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWP74597.1"
                     /translation="AEMQPGTQAKFLRVLEEGAFRRLGGKTEITVDVRVVAATNKDPV
                     AAVKDGAFREDLYYRLNVFTLAVPPLRQRVEDIPLLVTGFIEEFNAKYDKRITGVDDA
                     TLKILMSHAWPGNVRELRNVVERAFIACEGDVIVPRIPLGISPPVVAWHVDPGTLTFA
                     VGLPLREVEKQFVLRTLAAENNNKTRAAVRLEISTKTLHNMLRRWGLLHPQVLRS"
     gene            complement(653..1021)
                     /locus_tag="VNU03_08785"
     CDS             complement(653..1021)
                     /locus_tag="VNU03_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP74598.1"
                     /translation="MISIVDDDLSVRRALRRLIGSAGYLVETFASAPEFLRSAPSTRT
                     ACLVLDIYLEGMTGFELQEQLVADRVVIPIIFMTAHDDAATRERIRRSGAAGYLGKPF
                     DGQALLDAIHRVAGPGATGC"
     gene            complement(1140..1769)
                     /locus_tag="VNU03_08790"
     CDS             complement(1140..1769)
                     /locus_tag="VNU03_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP74599.1"
                     /translation="MTAATAPLVFVVDDDASVRKSLGRLIKTAGYEAEAFASVGEFLA
                     RRPYDGPCCLVLDVRMPGLTGLDLQEALRAAGQRLSIVFITGYRDVPVSVKAMKGGAV
                     DFLTKPVDEAVLLAAIRQAVARTQSDRRQLARLMEVQSRIKTLTPREAGVFALVVTGM
                     LNKQIGSELGIGEKTVKVHRARVMEKMRAGSLAELVRLAGEASVIAAGS"
     gene            complement(1766..3118)
                     /locus_tag="VNU03_08795"
     CDS             complement(1766..3118)
                     /locus_tag="VNU03_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP74600.1"
                     /translation="MWQVAVGAVFILSLLLLAIGLILERAWRRRAEVLLGERLRFETL
                     LSEQVATFSRISAADVDREIHRALRRIADLLAVDWGNLTEYSDDSRTARVTHWWVADG
                     VGPQPEAIGFEEIPWVVTRLRRGELVRFSRIEELPEGDAMVDRETYRRLGVKSQVAVP
                     LTVEGAVVGALAFSTVGTERAWRDEFVQRLHLLGDVFAHTLSRRRSELEGQRLRQDLA
                     HVGRVSTMGELTASLAHELNQPLTAILSNAQAAQRILVSDKADLAEIREILGDIVEDD
                     KRAGEVIHRLRGFLKKSNTELAALDIGELVAQVARLVSSDAIIRNVAIRLDLAPGLPP
                     VCGDRVQLQQVILNLLMNGLDAMRESGEGERALVLRTVDGPESVVVAVEDSGVGIDEA
                     DLDHVFHAFYTTKPDGLGMGLAIARSIVEAHGGHLEARNNPGCGATFSFTLPVIKATP
                     "
     gene            complement(3224..>3587)
                     /locus_tag="VNU03_08800"
     CDS             complement(3224..>3587)
                     /locus_tag="VNU03_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74601.1"
                     /translation="GEVVIREFHRFEGVSDPDDMCIVYAIEAQGGARGTLVDAFGVYS
                     DPVVSAFFQKVPIRGARRFGGGLDERPWEFGPPGEIRERKRRIPEPARFGAYRHGVPL
                     PVPSDPWQDEGGESGPGQ"
BASE COUNT          573 a         1337 c         1163 g          514 t
ORIGIN      
        1 gccgagatgc agcccggcac ccaggccaag ttcctccgcg tcctcgagga gggcgccttc
       61 cgccgcctcg gcggcaagac cgagatcacc gtcgacgtcc gcgtcgtcgc cgccaccaac
      121 aaggacccgg tcgcggcggt gaaggacggg gccttccgcg aagacctgta ctaccggctc
      181 aacgtcttca ccctcgcggt gcccccgctc cgccagcggg tcgaggacat ccccctcctc
      241 gtcaccggct tcatcgagga gttcaacgcc aagtacgaca agcgcatcac cggcgtcgac
      301 gacgccaccc tcaagatcct catgtcccac gcgtggccgg gcaacgtccg cgagctgcgc
      361 aacgtcgtgg agcgcgcgtt catcgcctgc gagggcgacg tgatcgtccc ccggatcccg
      421 ctcggcatct cgccgccggt ggtcgcgtgg cacgtcgacc ccggcacgct caccttcgcg
      481 gtcggcctgc ccctgcgcga ggtcgagaag cagttcgtgc tgcgcaccct ggccgccgag
      541 aacaacaaca agacgcgggc ggccgtccgg ctggagatca gcaccaagac cctgcacaac
      601 atgctccggc ggtggggact cctgcacccg caggtgcttc gcagctagtc ggtcagcacc
      661 ccgtcgcccc cggccccgcg acccgatgga tggcgtcgag cagggcctgg ccgtcgaatg
      721 gcttccccag gtagccggcc gccccggatc gccggatgcg ctcgcgcgtc gcggcgtcgt
      781 cgtgggcagt catgaagatg atggggatga cgacccgatc cgccaccagc tgctcctgga
      841 gctcgaaccc ggtcatgcct tcgaggtaga tgtcgaggac caggcacgcg gtgcgggtcg
      901 acggcgccga ccggaggaac tcgggggccg acgcgaacgt ctcgacgaga tacccggcgg
      961 agccgatcaa tcgacgcagg gcgcggcgca ccgacaggtc gtcgtccacg atcgagatca
     1021 tcgggccaga tttgagcatg gcatgggaac actccacccg atcctacagg gcggggccgg
     1081 gactggtagt ggaccttggt ccaccccgcg cgcccgcgac gcctcgacgc ccggctcgct
     1141 cacgatccgg ccgcgatcac gctcgcctcg ccggcgagcc ggaccagctc cgcgagggag
     1201 ccggcccgca tcttctccat gacccgggcc cgatgcacct tgaccgtctt ctcgccgatg
     1261 ccgagttcgg acccgatctg cttgttcagc atgccggtca ccaccagggc gaagactccg
     1321 gcctctctcg gggtcagggt cttgatgcga ctctgaacct ccatgagccg ggccagctgc
     1381 cgccggtccg actgggtccg ggccaccgct tgccggatcg ccgccaggag gaccgcctcg
     1441 tccaccggct tggtgaggaa atcgaccgcg ccacccttca tggccttgac gctcaccggg
     1501 acgtcccgat agccggtgat gaacacgatc gacaggcgct gcccggccgc ccggagggct
     1561 tcctgcaaat cgagtccggt cagcccgggc atgcgcacgt cgaggaccag gcagcacggg
     1621 ccgtcgtacg ggcggcgggc gaggaactcc ccgaccgacg cgaaggcctc cgcctcgtag
     1681 ccggcggtct tgatcagccg tccgaggctc ttgcggaccg acgcgtcgtc gtccaccacg
     1741 aacaccagcg gggcggtggc cgcggtcacg gcgtggcctt gatgaccggc agcgtgaacg
     1801 agaaggtggc cccgcacccg ggattgtttc tcgcctccag gtggcctccg tgcgcctcca
     1861 cgatggagcg cgcgatggcc agccccatcc cgagcccgtc gggcttcgtg gtatagaacg
     1921 cgtggaagac gtggtcgagg tcggcctcgt cgatgccgac ccccgagtcc tccaccgcca
     1981 ccaccacgct ctccggaccg tcgacggtcc ggagcaccag ggcccgctcg ccctcgccgg
     2041 attcccgcat cgcgtccagc ccgttcatga gcaggttcag gatcacctgc tgcagctgca
     2101 cccggtcgcc gcacaccggt gggagccccg gcgcgagatc gagccggatg gcgacgttgc
     2161 gaatgatcgc gtcactgctc acgagccggg ccacctgagc gaccagctcc ccgatgtcga
     2221 gcgccgcgag ctcggtattg ctcttcttga ggaagccacg cagccgatgg atcacctcgc
     2281 cggcgcgctt gtcgtcctcg acgatgtcgc ccaggatctc gcgaatctcg gccagatcgg
     2341 ccttgtcgga gacgaggatg cgctgagccg cctgcgcgtt gctgaggatc gcggtcaggg
     2401 gctggttcaa ctcgtgggcc agcgaggcgg tcagctcccc catcgtcgag acgcgcccca
     2461 cgtgggcgag gtcttgacgg aggcgctgtc cctccagctc ggatcggcgg cgcgacagcg
     2521 tgtgggcgaa cacgtccccg aggagatgga gtcgctgcac gaactcgtcc cgccaggccc
     2581 gctcggtccc gacggtgctg aacgcgaggg cgccgaccac cgccccctcc accgtcaacg
     2641 gcaccgcgac ctgcgacttg acgccgaggc ggcggtacgt ctcccggtcg accatggcgt
     2701 cgccttccgg aagctcctcg atccttgaaa accggaccag ctctccgcgg cgcagacgcg
     2761 tcaccaccca gggaatctcc tcgaatccga tcgcctccgg ctgcggcccc accccgtcgg
     2821 ccacccacca gtgcgtgacc cgggcggtcc ggctgtcatc ggagtactcg gtcaggttgc
     2881 cccaatcgac cgcgagcagg tcggcgatcc ggcgcagcgc ccggtggatc tcgcgatcga
     2941 cgtcggccgc cgagatgcgg ctgaaggtcg cgacctgctc ggacagcaag gtctcgaacc
     3001 ggagccgctc gccgagcaag acctccgccc ggcgccgcca ggcccgctcg agtatgagtc
     3061 cgatggcgag cagcagcagc gagaggatga ataccgcgcc cacggcgacc tgccacacga
     3121 cccaatcgcc cgacattcag tcgacactat agtctgccca gggccccccg gcaacgccgg
     3181 gacccggtat gagaccgggg tccaaagggg ggcgcgtaga cgatcactgc cccggtccgc
     3241 tctccccgcc ctcgtcttgc cacgggtcgg agggaaccgg gagcggaacg ccgtggcggt
     3301 aggcaccgaa gcgcgcgggc tccgggatcc gcctcttccg ctcccggatc tcgcccggcg
     3361 gaccgaattc ccacggtcgc tcgtccaggc ctccgccgaa ccgtcgggcc ccgcggatcg
     3421 gcaccttctg gaagaaagcg ctcaccaccg ggtcggagta gaccccgaag gcgtccacca
     3481 gggttccccg agcaccgcct tgcgcctcga tcgcgtagac gatgcacata tcgtcgggat
     3541 ccgagacccc ctcgaaccgg tggaactccc ggatcaccac ctcgccc
//
LOCUS       DATMCV010000379         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_219959, whole genome shotgun sequence.
ACCESSION   DATMCV010000379 DATMCV010000000
VERSION     DATMCV010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219959"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..768)
                     /locus_tag="VNU03_08805"
     CDS             complement(52..768)
                     /locus_tag="VNU03_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP74602.1"
                     /translation="MKLAGQVAVVTGAGRGIGRAIAGALGREGAAVVLAARSDSELEI
                     VAREIEQAGGRALAVPTDVRQEASVEALVRRALAEWRQVDVLVNAAGVATFAPVTDSK
                     LDDWDQMLAVNLRGAVLCCRAVLPAMVGRHRGTIINVGSVVTSRSLAGSAAYTASKYG
                     LLGFSRVLAEEMRPHGVRVGVLSAGATDTPLWDAMPAAPARDRMLRADQVAEAALLMA
                     GLGPNASLEEVTLLPAGGIL"
     gene            complement(765..1325)
                     /gene="folE"
                     /locus_tag="VNU03_08810"
     CDS             complement(765..1325)
                     /gene="folE"
                     /locus_tag="VNU03_08810"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWP74603.1"
                     /translation="MERLIRDMLKEMGEDPAREGLEKTPTRVAKAWEYLTSGYRQNVH
                     EVLNEALFTEEYDEMVVVKDIDFYSICEHHLLPFFGKCHIAYMPSRKIVGLSKLPRLV
                     EMFARRLQVQERFTTQVAHTLEEVLQPRGVAVVVEALHMCMLMRGVEKQNSKAVTSAM
                     LGAFRENAETRAEFMELIRARGGLVI"
     gene            complement(1353..1775)
                     /locus_tag="VNU03_08815"
     CDS             complement(1353..1775)
                     /locus_tag="VNU03_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWP74604.1"
                     /translation="MSAYAVTRAYHFSAAHRLANDAFSDEENAELYGQCFRQHGHNYL
                     LEVTVSGALDPATGMSVDIGVVDRAVKAAVLDRVDHYDLSATVPALAGVITTGENLAR
                     TFWEWLEGALPPGSLRRVTLVETANNVFEYRGESAPAR"
     gene            complement(1772..2173)
                     /locus_tag="VNU03_08820"
     CDS             complement(1772..2173)
                     /locus_tag="VNU03_08820"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWP74605.1"
                     /translation="MVYATRKFTFSAGHRYWRPEWSAEENARTFGSLTVAHGHNYALE
                     VTIRGAVDPRTGMVMDLGELKRVVGDAVIGRFDHADLNQDPLFPPGTIPTTENLVRVI
                     WELLGPKLGADRLHRLRLWEDPTFYVEYLGE"
     gene            complement(2179..>2975)
                     /locus_tag="VNU03_08825"
     CDS             complement(2179..>2975)
                     /locus_tag="VNU03_08825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP74606.1"
                     /translation="LRNERGRVRALLAVQARWERRNVERADLVMVTSRYSAEVAQREY
                     GVPPERLAVVPEPIDLEVWDDQFWRAPRRPRGAPVVLCVARMYPRKRIQDLMRAAGIL
                     RARIPGVQVRVVGRGPEWPMLSRLHAELGLGDAVALLGDLTRERLAEEYVNATVFCLP
                     SVQEGFGIVFLEAMAAELPVVACRIAAVPEVVLDGVTGLLSDPRDPGALAGLIERLIV
                     DPALARGLGREGRRRALGFTPRHVAERFLHAVHSSQDRLGQQARGG"
BASE COUNT          432 a         1115 c         1019 g          409 t
ORIGIN      
        1 atagcagaag gaccgtgggc ttgccgggcg ccctccctgc cggccggcgg cctagaggat
       61 gcctcccgcg ggcagcagcg tgacctcctc gaggctcgca ttcgggccca gtcccgccat
      121 gagcagcgcc gcctcggcca cctgatcggc ccggagcatg cggtcccggg ccggggccgc
      181 gggcatggcg tcccagagcg gggtgtcggt ggccccggcg gagagcacgc ccacgcggac
      241 cccgtggggc cgcatctcct cggcgagcac ccgggagaag cccagcaacc cgtacttgga
      301 ggcggtatag gccgcactgc cggccagcga ccggctcgtg accaccgagc ccacgttgat
      361 gatggtgccc cgatggcggc ccaccatggc cgggaggacc gcgcggcagc agaggaccgc
      421 gccgcggagg ttcacggcca gcatctggtc ccagtcgtcg agcttggagt cggttaccgg
      481 cgcgaaggtg gcgacgccgg ccgcgttgac cagaacgtcg acctgtcgcc actcggcgag
      541 cgcccggcgg accagcgcct ccacggaggc ctcctgccgt acgtcggtcg gcacggccag
      601 ggcccggccc cccgcctgct cgatctcgcg cgccacgatc tccagctcgg aatcggagcg
      661 cgcggccagc accaccgcgg cgccctcccg cccgagcgcg cccgcgatcg cccgaccgat
      721 gccccgcccg gcgccggtga cgaccgcgac ctggcccgcg agcttcaaat gacgagcccg
      781 ccgcgggccc ggatcagctc catgaactcg gcgcgcgtct ccgcgttctc gcggaaggcc
      841 ccgagcatgg ccgaggtgac cgccttggag ttctgcttct cgaccccgcg catcagcatg
      901 cacatgtgca gggcctcgac caccaccgcc accccccgcg gctggagcac ctcctcgagg
      961 gtgtgcgcga cctgggtggt gaatcgctcc tgcacctgca ggcgccgcgc gaacatctcg
     1021 accagccgcg gcagcttgga cagccccacg atcttgcgcg agggcatgta ggcgatgtgg
     1081 cacttgccga agaaggggag caggtgatgc tcgcagatgg agtagaagtc gatgtccttc
     1141 accaccacca tctcgtcgta ctcttcggtg aagagcgcct cgttgagcac ctcgtgcacg
     1201 ttctgccggt agcccgacgt caggtactcc cacgccttgg ccacgcgggt cggggtcttc
     1261 tcgagcccct cccgggccgg gtcctcgccc atctccttca gcatgtcccg gatgaggcgc
     1321 tccatcgtcg tgctctcatc tcccatggct cgtcacctcg cgggggcgga ctcgccgcgg
     1381 tattcgaaca cgttgttggc cgtctccacg agcgtcacgc gccggagact ccccggcggc
     1441 agcgcgccct cgagccactc ccagaaggtg cgggccaggt tctccccggt ggtgatcacc
     1501 cccgccagcg cgggcacggt ggcggagaga tcgtagtgat cgacccggtc cagcaccgcg
     1561 gccttcaccg cccggtcgac cactccgatg tcgaccgaca tgccggtggc gggatcgagg
     1621 gcgccggaca ccgtgacctc gagcaggtag ttgtggccgt gctggcggaa gcactgccca
     1681 tacagctccg cgttctcctc gtcggagaag gcgtcgttgg cgaggcggtg cgcggcactg
     1741 aagtggtaag cgcgggtgac cgcgtaggcg ctcattcgcc gagatactcg acatagaagg
     1801 tggggtcctc ccagagccgc agccggtgca gccgatcggc gccgagcttg gggccgagga
     1861 gctcccagat cacccgcacc aggttctcgg tggtgggaat cgtgccgggc gggaagagcg
     1921 ggtcctggtt gagatcggcg tggtcgaagc ggccgatcac cgcgtcgccc accacgcgct
     1981 tcagctcgcc caggtccatc accatgccgg tgcgcggatc gaccgccccg cggatcgtca
     2041 cctcgagcgc gtagttgtgg ccgtgggcca ccgtgagcga gccgaaggtg cgcgcgttct
     2101 cctccgcgct ccactccggg cgccagtagc ggtgccccgc cgagaacgtg aacttccggg
     2161 tcgcgtacac catgcggctc agcctcctcg cgcctgttgt cccaggcgat cctgcgacga
     2221 gtgtaccgcg tggaggaagc gctccgcgac gtgccgcggg gtgaacccga gcgcgcggcg
     2281 ccggccttcg cggcccaggc cgcgggccag cgccggatcc acgattagcc gttcgatgag
     2341 gccggcgagc gcgcccggat cccgcggatc gctcagcagg ccggtgaccc cgtcgagcac
     2401 cacctccggg accgcggcga tgcggcaggc caccaccggc agctcggccg ccatcgcctc
     2461 caggaacacg atgccgaatc cctcctgcac cgacggcagg cagaacacgg tcgcgttgac
     2521 gtactcctcg gcgaggcgct cacgggtgag atcgcccagc aaagccaccg cgtccccgag
     2581 gcccagctcg gcgtgcaggc gcgagagcat cggccactcc ggcccgcgcc ccaccacccg
     2641 gacctggacg cccgggatgc gggcgcggag gatcccggcc gcgcgcatca ggtcctggat
     2701 ccgcttgcgc gggtacatgc gggccacgca gagcaccacc ggggccccgc gcggccgccg
     2761 cggggcgcgc cagaactgat cgtcccacac ctcgaggtcg atgggctccg gcaccaccgc
     2821 gagccgctcc ggcggcacgc cgtattcccg ctgggcgacc tccgcggagt agcggctcgt
     2881 caccatgacg aggtcggccc gctcgacgtt gcgccgctcc cagcgcgcct gcaccgcgag
     2941 cagggcgcgc acgcggccgc gctcgttcct gagct
//
LOCUS       DATMCV010000380         3923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_486930, whole genome shotgun sequence.
ACCESSION   DATMCV010000380 DATMCV010000000
VERSION     DATMCV010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486930"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VNU03_08830"
     CDS             <1..218
                     /locus_tag="VNU03_08830"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74607.1"
                     /translation="RLATTHGIRLAVRVVDMVYNLAGATAVYDGNPIQRHFQDVHVIS
                     QHLQARLSHYELVGRHWLGLKIDEGRL"
     gene            232..1800
                     /locus_tag="VNU03_08835"
     CDS             232..1800
                     /locus_tag="VNU03_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74608.1"
                     /translation="MAAKDISRRIFLQTALASGALAPAGLLASRGAGPAWAQETPKKG
                     GTLRVGFYIEAATMDPHLSGSKIDRQVYHNIYEPLVVLDANLGIKPGLAESWQQPDAK
                     TLIFKLRRGVKFHDGTDFNAEAVKFNFNRMKTEPKSVRKGEVASIDTVDILDAYTVKL
                     NLKRPDAALLATLTDRAGMMVSPKTAQERGAELERNAKGAGTGPFVFVEWVKDDHLLI
                     RRNDSYWNKQGGPYLEQIRYRPIPDDTVKLQSLQAGEIQVMDYVQPRDVAAVKADKNV
                     VVLDVPSLADFAYQLNHTKPPFDNKALRQAVAYSLDLEQIVKGVWLNVGVPANGPIPP
                     TSWAYDRSIPFIKRDLAKVKAKLAEGGKAGGFTFTMTTNNIPINVQEAEVIQAQLAEA
                     GITMKIKLVDAGTLISDGNGKNFEMISYQWSGRPDPDGNTYQFYKTTPGTSLNWSGIS
                     NPQIDALLDKSREVSSQAERKKLFSQLVRILQDELPMVFIVHPIEPKAFSPRVQGYDP
                     IPDGMMRFKDVWLK"
     gene            1816..2760
                     /locus_tag="VNU03_08840"
     CDS             1816..2760
                     /locus_tag="VNU03_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015717097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74609.1"
                     /translation="MGRLILRRLAATIPVLLLVTAGVFALIHLTPGDPIDAMMAESVD
                     DTVKRELRRDLGLDRPLYLQYATWMGRLLQGDLGHSIRNREPVIENVSRRIKPSLQLA
                     GLAMAISLLVATPIGILSAARRNSTIDRVGTSFALFGICMPNFLIALLLIFFFGVTLR
                     WLPISGYVDPMEEPWEGLRSLTLPAITLGLALAAVITRTLRSSMLEALSEDYIRTARA
                     KGLSDGAVVRRHALKNALIPVVTVLGLQLGTLIGGAVITEYVFALPGVGRLVVDAVFA
                     RDYPLVQGVVLLIAVGFILSNLAVDLLYGFIDPRIRHR"
     gene            2726..3646
                     /locus_tag="VNU03_08845"
     CDS             2726..3646
                     /locus_tag="VNU03_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74610.1"
                     /translation="MASSTPGSGIAEPAAAALAPPVVPRPVRARRWRTLRHAARARLA
                     PFGAAVLLVAVAAALLAPLVAPHDPLKQDLNHALARPDRAHLLGTDNVGRDVLSRVIW
                     GTRVSLVAGLGSVGIAVLVGGLLGLLAGYAGGRVDGLVMRLMDAVLSFPPLVLALALG
                     AVLGAGLTGVVIALGVVYTPTFARLMRGQVLSITAREYVDAARALGVPGWRIAWSHVL
                     PNATAPIVVQASLSVAFAILAEASLSFLGLGVQPPGASWGSMINAGRGYLQQAPWIVF
                     GPGAALFVTVVGLNFVGDAVRDALDPRLRV"
     gene            3662..>3923
                     /locus_tag="VNU03_08850"
     CDS             3662..>3923
                     /locus_tag="VNU03_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HWP74611.1"
                     /translation="MARIATLVAVALLLAASAALAAAGEREEANKKAVVEFYDKALNQ
                     KDFEAAAPYFGSRYVQHNPNAPDGIDGFKAFLGFLTEKFPQSR"
BASE COUNT          587 a         1395 c         1346 g          595 t
ORIGIN      
        1 tgcggctcgc caccacccac gggatccgcc tcgcggtccg ggtggtggac atggtctaca
       61 acctggccgg cgcgaccgcg gtgtacgacg gcaacccgat ccagcgccac ttccaggacg
      121 tgcacgtgat cagccagcac ctgcaggccc ggctgtcgca ctacgagctg gtcgggcggc
      181 actggctcgg cctcaagatc gacgagggtc ggctctaaca ggaggagacg aatggcggcc
      241 aaggacatct cacgacgcat ctttctgcag accgcgctcg cctcgggcgc gctggccccc
      301 gcggggctgc tcgcgtcccg cggggcggga ccggcgtggg cccaggagac gccgaagaag
      361 ggcggcacgc tgcgcgtcgg cttctacatc gaggcggcca ccatggatcc gcacctgtcg
      421 ggcagcaaga tcgaccggca ggtctaccac aacatctacg agccgctggt cgtgctcgac
      481 gccaacctcg ggatcaagcc cggcctggcc gagtcctggc agcagccgga cgcgaagacg
      541 ctgatcttca agctccgccg cggggtgaag ttccacgacg gcaccgactt caacgcggag
      601 gcggtgaagt tcaacttcaa ccgcatgaag accgagccca agtcggtgcg caagggcgag
      661 gtggccagca tcgacacggt cgacatcctc gacgcctata cggtcaagct caatctcaaa
      721 cgcccggatg cggcgttact cgcgacgctc accgatcgcg cggggatgat ggtatcgccc
      781 aagaccgcgc aggagcgcgg ggccgagctg gagcgcaacg ccaaaggcgc ggggaccggg
      841 ccgttcgtgt tcgtggagtg ggtgaaggac gaccacctgc tgatccggcg caacgacagc
      901 tactggaaca agcagggcgg cccctacctc gagcagatcc gctaccggcc gatcccggac
      961 gacaccgtca agctgcagag cctgcaggcg ggcgagatcc aggtgatgga ctacgtgcag
     1021 ccccgcgacg tggccgcggt caaggcggac aagaacgtgg tggtgctcga cgtgccgtcg
     1081 ctggccgact tcgcctacca gctcaaccac accaagccgc ccttcgacaa caaggcgctc
     1141 cgccaggcgg tggcctactc gctcgacctc gagcagatcg tcaagggggt ctggctcaac
     1201 gtgggcgtgc ccgccaacgg gccgatcccg cccaccagct gggcctacga ccgctcgatc
     1261 ccgttcatca agcgcgacct cgccaaggtc aaggccaagc tcgcggaagg cggcaaggcg
     1321 ggcggcttca cgttcacgat gaccaccaac aacatcccga tcaacgtgca ggaggccgag
     1381 gtcatccagg cccagctcgc ggaggcgggg atcaccatga agatcaagct ggtggacgcg
     1441 ggcaccctga tctcggacgg caacggcaag aacttcgaga tgatcagcta ccagtggagc
     1501 gggcggcccg atcccgacgg caacacctac cagttctaca agaccacgcc cgggacgtcg
     1561 ctcaactggt cgggcatctc caatccgcag atcgacgccc tgctcgacaa gtctcgcgag
     1621 gtctcgagcc aggccgagcg caagaagctc ttcagccagc tcgtgcggat cctgcaggac
     1681 gagctgccga tggtgttcat cgtccacccg atcgagccga aggccttcag cccccgggtg
     1741 cagggctacg acccgatccc ggacgggatg atgcgcttca aggacgtctg gctgaagtag
     1801 cgtccgggcg gtcccgtggg acgcctgatc ctccgccggc tcgccgcgac gatcccggtg
     1861 ctgctgctgg tcaccgccgg ggtgttcgcc ctgatccacc tgacccccgg cgacccgatc
     1921 gacgcgatga tggcggagtc ggtcgacgac accgtcaagc gcgagctgcg gcgggacctc
     1981 ggcctcgacc ggccgctcta cctccagtac gcgacgtgga tggggcgcct gctccagggc
     2041 gacctcggcc actcgatccg caatcgggag ccggtgatcg agaacgtgag ccgccggatc
     2101 aagccgagcc tgcagctggc cgggctcgcc atggcgatct cgctgctggt ggccaccccg
     2161 atcggcatcc tctccgccgc gcgccgcaac tcgacgatcg accgcgtcgg cacctcgttc
     2221 gccctcttcg gcatctgcat gccgaacttc ctgatcgcgc tcctgctgat cttcttcttc
     2281 ggggtgaccc tgcgctggct gccgatctcg ggctacgtcg atccgatgga ggagccgtgg
     2341 gagggcctcc gctcgctgac ccttcccgcg atcaccctcg ggctcgccct cgccgcggtg
     2401 atcacgcgga ccctgcgctc gagcatgctc gaggcgctct ccgaggacta catccggacc
     2461 gcccgcgcca aggggctctc cgacggcgcg gtggtgcgcc gccacgcgct gaagaacgca
     2521 ctgatcccgg tggtcaccgt gctcgggctg cagctcggca ccttgatcgg cggggcggtc
     2581 atcaccgagt acgtgttcgc gctcccgggg gtgggccgcc tggtggtgga cgcggtcttc
     2641 gcccgcgact acccgctggt gcagggcgtg gtgctgctga tcgcggtggg cttcatcctg
     2701 agcaacctcg cggtggacct gctctatggc ttcatcgacc cccggatccg gcatcgctga
     2761 gccggcggcc gcggccctgg cgccgccggt cgttccccgt ccggtgcgcg cgcgccgctg
     2821 gcgcaccctg cgccacgccg cgcgcgcgcg gctggccccg ttcggggccg cggtcctgct
     2881 cgtcgcggtg gccgcggccc tgctggcgcc gctggtcgcg ccccacgatc cgctcaagca
     2941 ggacctgaac cacgcgctcg cccggcccga ccgcgcccat ctcctgggca ccgacaacgt
     3001 gggccgcgac gtgctctcgc gggtgatctg gggcacgcgg gtgtcgctgg tggcgggcct
     3061 cgggtcggtc ggcatcgcgg tgctggtcgg aggcctgctg ggcctgctgg ccggctacgc
     3121 gggcgggcgg gtcgacgggc tggtgatgcg gctgatggac gcggtgctgt cgttcccgcc
     3181 gctggtgctg gctctcgccc tgggcgcggt gctcggcgcc gggctcaccg gcgtcgtcat
     3241 cgccctcggc gtggtctaca cgccgacgtt cgcgcggctg atgaggggcc aggtgctctc
     3301 gatcactgcg cgcgagtacg tggacgcggc ccgcgccctc ggcgtgcccg gctggcgcat
     3361 cgcgtggtcg cacgtgctcc ccaacgcgac cgcgcccatc gtggtgcagg cctcgctgag
     3421 cgtggccttc gcgatcctcg ccgaggcgtc cctgtcgttc ctcggcctgg gcgtgcagcc
     3481 gcccggcgcc tcctggggca gcatgatcaa cgcgggccgc gggtacctgc agcaggcccc
     3541 ctggatcgtg ttcggccccg gggccgccct cttcgtcacg gtggtcgggc tcaacttcgt
     3601 gggcgacgcg gtccgcgacg ccctcgaccc gcggctgcgg gtctagggta tgatcggggc
     3661 catggcccgg atcgcgaccc tggtggcggt ggccctgctg ctggcggcga gcgccgcgct
     3721 cgcggcggcg ggcgagcggg aagaggccaa caagaaggcg gtcgtcgagt tctatgacaa
     3781 ggcgctcaac cagaaggact tcgaggcggc ggccccgtac ttcgggagcc gctacgtgca
     3841 gcacaacccg aacgcccccg acggcatcga tggcttcaag gcgttcctcg ggttcctgac
     3901 cgagaagttc ccccagtcgc gca
//
LOCUS       DATMCV010000381         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_219980, whole genome shotgun sequence.
ACCESSION   DATMCV010000381 DATMCV010000000
VERSION     DATMCV010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219980"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..946
                     /locus_tag="VNU03_08855"
     CDS             29..946
                     /locus_tag="VNU03_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74612.1"
                     /translation="MRRLTVLMPFHTPFYTPLAAGVALGHFRDAGLDVAAVPAATFGK
                     PTIDALLGGDIEISLGGLMRSFELADRTGQIVVHFAEVCSRNGFFLLAREPRPAFRWS
                     DLVGKTVLSFAEAPTPWQCMLTVLRGHGVDPAQVRIERHRPGPEAVAAFLAGTGDFLE
                     QPQPIIERLLTEGRGHLVASMGEATGPVPFSSYMTTPAFLAREPDVIARFTRATYRTQ
                     RWLASQGAGDIARAVAPTFPETELPLLERAIGRYLGQGTWARDPLLRREGYDYLERIL
                     LDGAFIRRGRPYEDLVDTAAARQVMAEAP"
     gene            943..2004
                     /locus_tag="VNU03_08860"
     CDS             943..2004
                     /locus_tag="VNU03_08860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP74613.1"
                     /translation="MKFGIGFFPDGGPDRISARQYFEEALALAERADALGYDSVKMVE
                     HHFTSYGGYSPDPSVFLAACAQRAPRMRMVTGAVLPAFNHPLKLAGQLAMLDALCGGR
                     LDVGVARAFMPYEFDGFGVSMEESRPRFEEGIQALRRLWTEERVTFEGRFHRFRDVTV
                     LPRPTQRPHPPIWVAAVVSPESFVWAGEQGYHMMVVPYLGEHHELAEKLQLYRRAYRE
                     HGHEATRGPGEVMMVLHLYVAPTAREAREEGRAYMEQYLLNFRASARAWTGRESGQYK
                     AYSGLEQMLAEITYERVLDETRAIIGDPEGAARQIAYVRDTFGDVHASFQINFGVMEE
                     ARARRSIELFAEQVMPRFR"
     gene            complement(2012..>2500)
                     /locus_tag="VNU03_08865"
     CDS             complement(2012..>2500)
                     /locus_tag="VNU03_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74614.1"
                     /translation="LDGDGRMLDNVRVARPRALAVDESDHLWVGSDGAAEAPWQAGPG
                     AIWRVSPRGEPRLVLEGPMAQALAAGPAGLLLVADRQGSEIFALTADGGRTSLARFTD
                     GDAPRGLAFVPATAETRAAGLAGDLLVAVVRSGAFQLNEIVRIAGPFADLTARPATPP
                     TR"
BASE COUNT          325 a          885 c          938 g          352 t
ORIGIN      
        1 tggtcgatcg cttcgtgaag gaggagctgt gaggcgcctc accgtcctga tgccattcca
       61 cacgccgttc tacactcctc tggccgccgg cgtggcgctc ggccacttcc gggacgccgg
      121 gctcgacgtc gccgcggtgc ccgcggccac gttcggcaag ccgacgatcg acgcgctcct
      181 cggcggcgac atcgagatca gcctgggcgg gctcatgcgg agcttcgagc tggccgaccg
      241 gaccgggcag atcgtggtcc acttcgccga ggtgtgcagc cgcaacgggt tcttcctgct
      301 cgcgcgcgag ccgcgcccgg ccttccgctg gagtgacctg gtcgggaaga ccgtgctgtc
      361 cttcgcggag gcgccgaccc cgtggcagtg catgttgacc gtgctccgtg gccacggcgt
      421 cgatccggcc caggtgcgga tcgagcggca tcgcccggga cccgaggcgg tggccgcgtt
      481 cctggccggg accggcgact tcctggagca gccccagccg atcatcgagc gcctcctcac
      541 cgaagggcgc ggacacctcg tcgcctcgat gggcgaggcg accgggccgg tgccgttctc
      601 ctcctacatg acgacgcccg ccttcctcgc ccgcgagccc gacgtgatcg cccgcttcac
      661 ccgcgcgacc taccggaccc agcgctggct cgcctcgcag ggggccggtg acatcgcccg
      721 cgcggtcgcg ccgactttcc cggagacgga gctgccgctg ctcgagcggg cgatcggccg
      781 gtacctcggg cagggaacct gggcgcggga cccgctgctg cggcgggagg ggtacgacta
      841 cctcgagcgc atcctgctgg acggcgcgtt catccgccgc gggcggccgt acgaggacct
      901 ggtcgacacc gcggcggccc ggcaggtgat ggcggaggcg ccgtgaagtt cggcatcggc
      961 ttcttccccg acggcggccc ggaccggatc tcggcccggc agtacttcga ggaggcgctg
     1021 gccctggccg agcgggcgga cgccctcggc tacgatagcg tgaagatggt cgagcaccac
     1081 ttcacgtcct acggcggcta cagccccgac ccgtccgtgt tcctggccgc gtgcgcccag
     1141 cgagcgccgc gcatgcggat ggtcaccggc gcggtcctgc ccgcgttcaa ccatcccctg
     1201 aagctggccg gccagctcgc gatgctcgac gcgctctgcg ggggacggct cgacgtgggg
     1261 gtggcgcggg ccttcatgcc ctacgagttc gacggcttcg gcgtctccat ggaggagagc
     1321 cggccgcgct tcgaggaggg aatccaggcc ctgcggaggc tgtggaccga ggagcgcgtg
     1381 acgttcgagg gccgcttcca ccgcttccgc gacgtcacgg tgctgccgcg tccgacccag
     1441 cgtccgcatc cgccgatctg ggtggcggcg gtggtgagcc cggagtcgtt cgtgtgggcc
     1501 ggcgagcagg gctaccacat gatggtggtg ccgtacctgg gcgagcatca cgagctggcc
     1561 gagaagctcc agctctaccg ccgcgcgtac cgcgagcacg ggcacgaagc gacgcgcggc
     1621 cccggcgagg tgatgatggt gctccatctc tacgtggcgc cgaccgcgcg cgaggcgcgg
     1681 gaggaaggcc gcgcctacat ggagcagtac ctcctcaact tccgtgcctc cgcgcgcgcg
     1741 tggacgggac gggagtcggg gcagtacaag gcctactccg ggctcgagca gatgctggcg
     1801 gagatcacct acgagcgggt gctcgacgag acgcgcgcga tcatcggcga tcccgagggc
     1861 gcggcgcggc agatcgcgta cgtgcgcgac accttcggcg acgtgcacgc gagcttccag
     1921 atcaacttcg gggtgatgga ggaggcgcgc gcgcggcgaa gcatcgagct gttcgccgag
     1981 caggtgatgc cgcgcttccg ctagtgggtg tttagcgggt gggcggggtg gccgggcggg
     2041 cggtgaggtc ggcgaagggg cccgcgatgc gcacgatctc gttgagctgg aaggcgcccg
     2101 agcggacgac cgcgaccagc aggtcgccgg ccaggccggc cgcgcgcgtc tccgcggtgg
     2161 cgggcacgaa ggccaggcct cgcggcgcgt cgccgtcggt gaagcgcgcc aggctggtgc
     2221 gccccccatc ggcggtgagg gcgaagatct cgctgccctg ccggtcggcc acgaggagca
     2281 gcccggccgg tccggccgcc agggcctgcg ccatcgggcc ctcgaggacg aggcggggct
     2341 cgccgcgagg gctcacccgc cagatcgcgc cggggcccgc ctgccacggc gcctccgcgg
     2401 cgccgtcgct gccgacccag aggtggtccg attcgtccac cgcgagcgcg cggggcctcg
     2461 cgacccgcac gttgtcgagc atgcgcccgt cgccgtcgag
//
LOCUS       DATMCV010000382         3931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_886351, whole genome shotgun sequence.
ACCESSION   DATMCV010000382 DATMCV010000000
VERSION     DATMCV010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886351"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..227
                     /locus_tag="VNU03_08870"
     CDS             84..227
                     /locus_tag="VNU03_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74615.1"
                     /translation="MKQRRKPTHTPIHMKVRKPMPAKPPKVEVPRTVYRRRPKHRRPA
                     EAE"
     gene            227..1486
                     /gene="thiI"
                     /locus_tag="VNU03_08875"
     CDS             227..1486
                     /gene="thiI"
                     /locus_tag="VNU03_08875"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HWP74616.1"
                     /translation="MAGPSPGHQTVPAAILHYHEISLKRGNRPLFLRHLQENLLRAIG
                     DLGPVRLVQLTGRIMLDLSRHPDPARLRERLARVCGVANFSLALRTGVSREALRAAVG
                     QAIEGRLFRTFRISARRAFKTFPMTSVELNRDLGAHVLALRPDVRVSLEHAELDVRVE
                     VLPGEAFVYADRDQGAGGLPVGASGTVAALLSGGIDSPVAAWRLMKRGCRVVFVHFHS
                     APYLPDLSRPKARELVTRLTQWQYHSRLFLVPFGEIQREVVLAVPPPARVVVYRRLMV
                     RIAERLARHAGAPALITGESLGQVASQTLANITRIDEVAGMPILRPLIGMDKLEITEQ
                     AHALGTYEISIEPDADCCTLFTPRHPDTRMEAAAVAAMEARLDIERLVTLGVETAEQE
                     TFEFPAGAGPYPMRGERRRSVRESISE"
     gene            1483..2865
                     /locus_tag="VNU03_08880"
     CDS             1483..2865
                     /locus_tag="VNU03_08880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP74617.1"
                     /translation="MTATLTFLGAAGGTVTGSKHLLEDGASRLLLDCGLFQGLKPLRL
                     RNWAPPPVDPKTLHAVVLSHAHIDHSGYLPRLCRDGFTGVIYCTGGTLDLLRIMLPDA
                     AHLQEEEAAFANRHRTSKHDPALPLYTVADADRVLAQSRRVAFGERFTPAPGVEARFT
                     PSGHILGAGLVTCVIGGRRLVFSGDLGRYDVPIMVDPDPVAEADVLLVESTYGNRVHP
                     PDDPVDRLAAAVRRAVDQKGWLLIPAFAVGRSQEILHDLRALETKGAIPSLPVFLDSP
                     MGIQATAIYASHTEEHDAALIALEADGTRPFAPRRLTLARTVDDSKRLNDTPGPRIIV
                     AGSGMATGGRILHHLRRLLPDPRTTVLFVGYQAAGTRGRRLKDGATEMKMLGAEVPVR
                     AEIMVSDAYSAHADREEILRWLGGFTRPPGMTYVVHGERDAASALRDAITSRLGWPAA
                     VAEDGQRVSL"
     gene            2943..>3931
                     /locus_tag="VNU03_08885"
     CDS             2943..>3931
                     /locus_tag="VNU03_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HWP74618.1"
                     /translation="MIGVERIPPTGPLIVAANHHNALVDGVLLLGHFPRRLVSVAKAP
                     LFSNPLIGPFLRLIGALPVHRRQEGGTDPARNAALFEASSATFRSGQAILIFPEGVSQ
                     PEPLLMPLRTGAARMLLAAEADAVGPVTLLPVGLMFHEPGTFRTGWAVVSIGEPVPVA
                     DCVALAAASPEEAARRLTARLGEAMRGLIALVDDRQTLRLVEHAERLWRQERGGEWDA
                     SARADWRRRATRAYRYLSHHDATRLSRLRGQMERYVKDLDEAGLRTAVLLKPPAPSVA
                     LRYAGGQLAALLGGLPLALWGLASHTLPYWLTWAAVRLLRPEGDVEATYKLVAG"
BASE COUNT          498 a         1474 c         1421 g          538 t
ORIGIN      
        1 caaggcggcg ggccgtcccg ccgggatcga caccttcccg ggcgcgccct attgaccccg
       61 gaccggcggg atatacttgc cggatgaagc agcgccggaa gcccacccac acgcccatcc
      121 acatgaaggt ccgcaagccg atgcccgcga agccgcccaa ggtggaggtg ccccggacgg
      181 tctaccggcg gcggcccaag catcgccggc cggcggaggc cgagtgatgg cgggcccgtc
      241 ccccgggcat caaacggtgc ccgccgcgat cctccactac cacgagatca gcctcaagcg
      301 gggcaaccgg cccctcttcc tccggcacct gcaagaaaac ctgctgcgcg cgatcggcga
      361 tctcggtccg gtgcggctcg tgcagctcac cgggcgcatc atgctggatc tgtcacgcca
      421 cccggatccg gcccgcctgc gcgagcggct cgcccgcgtg tgcggggtgg ccaacttctc
      481 gctggccctg cgcaccggcg tctcgcgcga ggcgctgcgg gccgcggtgg ggcaggcgat
      541 cgaggggcgc ctattccgga ccttccggat ctcggcccgg cgcgccttca agaccttccc
      601 gatgacctcg gtcgagctca atcgcgacct gggcgcgcac gtgctcgcgc tccgacccga
      661 cgtgcgggtc agcctcgagc acgccgagct cgacgtgcgg gtggaggtgc tgcccggaga
      721 ggcgttcgtc tacgcggacc gcgaccaggg ggcgggcggc ctgccggtgg gagcgagcgg
      781 cacggtggcc gcgctgctgt cgggcggcat cgactcgccg gtggcggcgt ggcgcctcat
      841 gaagcggggc tgccgcgtgg tcttcgtgca cttccacagc gcgccctacc tgcccgatct
      901 ctcccgcccc aaggcgcgcg agctggtgac ccgtctcacc cagtggcagt accactcgcg
      961 cctcttcctc gtccccttcg gggagatcca gcgcgaggtg gtgctggcgg tgcccccgcc
     1021 cgcccgcgtg gtcgtctacc ggcggctcat ggtgcgcatc gccgagcgcc tggcccggca
     1081 cgcgggcgcg cccgcgctga tcaccggcga gagcctgggg caggtggcct cgcagacgct
     1141 ggccaacatc acccgcatcg acgaggtggc cggcatgccg atcctgcggc ccctgatcgg
     1201 catggacaag ctcgagatca ccgagcaggc gcacgcgctc gggacgtacg agatctccat
     1261 cgagccggac gcggactgct gcacgctctt caccccgcgc catccggata cccggatgga
     1321 ggccgccgcg gtggcggcga tggaggcgcg gctcgacatc gagcgcctgg tcaccctggg
     1381 ggtggagacc gcggagcagg agacgttcga gttcccggcg ggggcggggc cgtacccgat
     1441 gcgcggcgag cgccggcggt cggtgcggga gtccatctcc gagtgaccgc gaccctgacc
     1501 ttcctcgggg cggccggggg caccgtgacc ggctccaagc atctgctcga ggacggcgcg
     1561 agccggctcc tgctcgactg cgggctcttc cagggtctca agcccctgcg cctgcgcaac
     1621 tgggccccgc cgccggtgga tccgaagacg ctgcacgcgg tcgtgctgag ccacgcccac
     1681 atcgaccact ccggctacct gccgcgcctc tgccgcgacg gcttcaccgg agtgatctac
     1741 tgcaccggcg ggacgctcga tctcctccgg atcatgctgc cggacgcggc gcacctccag
     1801 gaggaggagg ccgcgttcgc gaatcggcac cggacgagca agcacgaccc cgcgctgccg
     1861 ctctacacgg tggcggacgc cgaccgtgtg ctcgcccagt cccgccgcgt ggccttcggg
     1921 gagcgcttca cgcccgcgcc cggcgtcgag gcccgcttca cgccgtcggg gcacatcctg
     1981 ggagcggggc tcgtcacgtg cgtgatcggc gggcggcgcc tcgtgttctc cggcgatctc
     2041 ggccgctacg acgtgccgat catggtggac ccggacccgg tggcggaggc cgacgtgctg
     2101 ctggtggagt ccacctacgg caatcgcgtg cacccaccgg acgatcccgt cgaccggctc
     2161 gcggccgcgg tgcgacgggc ggtcgatcag aagggctggc tcctcatccc cgccttcgca
     2221 gtgggccgct cgcaggagat cctgcacgac ctgcgcgccc tcgagacgaa aggggcgatc
     2281 ccgagcctcc cggtcttcct cgacagcccg atggggatcc aggccaccgc gatctacgcg
     2341 agccacaccg aggagcacga cgccgcgctg atcgcgctgg aagcggacgg cacgcggccc
     2401 ttcgcgccgc gccgcttgac cctggcccgc acggtggacg attccaagcg gctgaacgac
     2461 acgccgggcc cgcgcatcat cgtggcgggc agcggcatgg ccaccggagg gcgcatcctc
     2521 caccacctga ggcgcctcct gcccgatccc cgcaccaccg tgctcttcgt cggctaccag
     2581 gccgcgggca cccgcgggcg ccggctcaag gacggcgcga ccgagatgaa gatgctgggc
     2641 gcggaggtcc cggtgcgcgc ggagatcatg gtgagcgacg cgtactcggc gcacgccgac
     2701 cgcgaggaga tcctgcgctg gctcggcggc ttcacgcggc cgcccggcat gacctacgtc
     2761 gtccacggcg agcgggacgc ggcctccgcg ctccgcgatg cgatcacgag ccgtctgggc
     2821 tggccggccg ccgtggccga ggacggccag cgcgtgtcgc tctgagcgac gcccccgcgc
     2881 cctatcggct ggtccgggcg ctggcccggc tgctgctcgc gctcttctat cgtcgcgtcg
     2941 acgtgatcgg cgtcgagcgc atcccgccca cggggccgct catcgtggcc gccaaccacc
     3001 acaacgcgct cgtggacggg gtgctgctgc tgggccactt tccccggcgc ctggtctcgg
     3061 tggccaaggc gccgctcttc tcgaatcccc tgatcgggcc cttcctccgc ctgatcggcg
     3121 cgctgcccgt ccaccgtcgc caggagggcg gcaccgatcc ggcgcgcaat gccgcgctct
     3181 tcgaggcctc gtcggcgact tttcggagcg gccaggcgat cctgatcttc ccggagggcg
     3241 tgagccagcc cgagccgctg ctgatgccgc tccggaccgg cgcggcgcgg atgctgctgg
     3301 ccgcggaggc ggacgcggtc ggtcccgtga ccctgctgcc ggtcgggctc atgttccacg
     3361 agccggggac gttccgcacc ggctgggccg tggtctcgat cggcgagccg gtgccggtgg
     3421 ccgactgcgt ggcgctcgcg gccgcctcgc ccgaggaggc cgcgcgccgg ctcaccgcgc
     3481 ggctcggaga agcgatgcgc gggctcatcg cgctggtgga cgatcgccag acgctgcggc
     3541 tcgtcgagca cgccgagcgg ctctggcgcc aggagcgggg cggggagtgg gacgcgagcg
     3601 cgcgcgcgga ctggcggcgg cgcgccacgc gcgcctatcg gtacctgagc caccacgacg
     3661 cgacgcgcct ctcgcggctg cgcggccaga tggagcgcta cgtgaaggac ctggacgagg
     3721 cgggcctgcg caccgcggtg ctgctgaagc cgccggcccc gagtgtggcg ctgcgctacg
     3781 cgggcggcca gctcgcggcc ctgctcggcg ggctcccgct ggcgctctgg gggctcgcca
     3841 gccacacgct gccgtactgg ctcacctggg ccgcggtgcg gctgctccgg cccgagggcg
     3901 acgtggaggc cacctacaag ctggtggccg g
//
LOCUS       DATMCV010000383         3201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_288555, whole genome shotgun sequence.
ACCESSION   DATMCV010000383 DATMCV010000000
VERSION     DATMCV010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288555"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VNU03_08890"
     CDS             <1..575
                     /locus_tag="VNU03_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74619.1"
                     /translation="TPQWAKIKRTGAQATIGEIYGSSGPILFNQWFELKVPSVVAHMG
                     ATVSAQTFIDQHVKQTDLAIVNNRWWPAKYSAVSEPMMAAYKKKTGTDPTNFAVQAHD
                     ATLVLLDAIQKAGSLDPQKIQAALESTTFVTAWGTRKFTPLAEGHRMPIQTVVVQIQS
                     GKKVPIYPESVAAAAGGKYVPVPPYAWDKK"
     gene            560..1315
                     /locus_tag="VNU03_08895"
     CDS             560..1315
                     /locus_tag="VNU03_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74620.1"
                     /translation="MGQEVAPPPKLALAGLSKRFGGLRAVSACSFDIAPGTVVGLIGP
                     NGSGKSTVFNLVTGLLRPDEGTILYDGRRIDGLATHEIARRGIGRTFQAVKIFRDLPV
                     RENLAIAAMGRRLHGWEPRAREWLGRMGLSHLADTHAGSLSIGQQRLLELTMNLVIDP
                     EFLLLDEPLAGVHPVVRRQIAETLSDLRARGRTLLIIEHHMPFVMGLCDKIVVMDHGE
                     KIAEGPPAAIRADARVIAALLGRAEGQGDDARA"
     gene            1302..2042
                     /locus_tag="VNU03_08900"
     CDS             1302..2042
                     /locus_tag="VNU03_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74621.1"
                     /translation="MLALEGVRSGYGKLEIVQAASLEVGDGAIVGIIGPNGAGKSTLL
                     KTAFGYLPPFAGRIALDGREIAGRRPDEIMRLGVGYLAQAGGLFAEMTVHDNLVLGGY
                     TVSRADKRRALDAVYARFPLFRDRGRQLAGALSGGEQRLLAIARALIVRPRLLLLDEP
                     SAALAPRFIDLIYATLTALNRDGLAMLIVEQNVEAILAVAHRVFVLDLGRNAFDGTPA
                     ELRASDRIRRLYLGEEGGEDEPAAGPVR"
     gene            2042..2926
                     /locus_tag="VNU03_08905"
     CDS             2042..2926
                     /locus_tag="VNU03_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74622.1"
                     /translation="MLESLLEFLIQGVVLGGLYALFAVGLSLIFGVLDVINVAHGEFF
                     AVGGYLAFAAVVLLHLPGAAGVLGAAVGTFLLGLCVYPLLIAPLKRRLGGRPQGPLYL
                     VLTLGLSTFLQSSLLAIAGGDYLRVPPQVSGILDLGVTAVSHQRLLVLGVAAALLTAL
                     FAFLRLHPVGLAVRAVALNPDAAQAMGINLPRIFSLTLALGVALAGVGGALMAPLFNV
                     YPAVGFPLTIKAFAITILGGMGNPVGALVASFLISIAESLSVMVIPSQWQNLIAFAVM
                     IVMLLVRPRGLFGRPVPR"
     gene            2923..>3201
                     /locus_tag="VNU03_08910"
     CDS             2923..>3201
                     /locus_tag="VNU03_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74623.1"
                     /translation="MSRRALALVAAAGVALLPVLLPHPFVLTIATQAAIWALLAASWD
                     LLSGYTGQISFGHAGFFALGAYAAAAATKHAGVSPWLGLLVGATIAAAV"
BASE COUNT          409 a         1197 c         1097 g          498 t
ORIGIN      
        1 tgaccccgca gtgggccaag atcaagcgca ccggggccca ggccaccatc ggcgagatct
       61 acgggtcgag cgggccgatt ctgttcaatc agtggttcga gctcaaggtg ccgtcggtgg
      121 tcgcccacat gggcgcgacg gtgagcgcgc aaaccttcat cgaccagcac gtcaagcaga
      181 cggatctcgc catcgtcaac aatcgctggt ggccggccaa gtactccgcg gtgtcggagc
      241 cgatgatggc ggcttacaag aagaagaccg gcaccgatcc caccaacttc gccgtgcagg
      301 cccacgacgc caccctggtg ctgctcgacg cgatccagaa ggcgggcagc ctcgatcccc
      361 agaagatcca ggccgcgctc gagagcacca ccttcgtgac cgcgtggggc acccgcaagt
      421 tcaccccgct cgccgagggg caccgcatgc ccatccagac cgtggtggtg cagatccagt
      481 ccggcaagaa ggtgccgatc tacccggagt cggtcgcggc ggccgcgggc ggcaagtacg
      541 tgccggtgcc cccgtacgcg tgggacaaga agtagcgccc ccgccgaagc tcgccctcgc
      601 cggcctcagc aagcggttcg gcggcctccg cgcggtgtcc gcctgctcgt tcgacatcgc
      661 gcccggcacg gtggtgggcc tcatcggccc caacggctcc ggcaagagca cggtcttcaa
      721 cctggtgacc ggcctcctgc gtcccgacga ggggacgatc ctgtacgacg gccggcgcat
      781 cgacggcctc gccacccacg agatcgcccg ccgcgggatc ggccgcacgt tccaggcggt
      841 gaagatcttc cgcgacctgc cggtgcgcga gaacctcgcc atcgcggcca tggggcggag
      901 gctccacggc tgggagccgc gcgcgcggga gtggctcggc cgcatggggc tgagccacct
      961 ggccgatacc cacgcgggca gcctctccat cggccagcag cgcctgctcg agctgacgat
     1021 gaacctggtg atcgatcccg agttcctgct gctcgacgag cccctcgccg gcgtgcaccc
     1081 ggtggtccgc cgtcagatcg cggagaccct gagcgacctg cgggcccgcg gccgcacgct
     1141 cctgatcatc gagcaccaca tgccgttcgt gatgggcctc tgcgacaaga tcgtggtgat
     1201 ggaccacggc gagaagatcg cggaggggcc gcccgccgcg atccgcgccg acgcgcgcgt
     1261 gatcgcggcg ctgctcgggc gggcggaggg ccagggcgac gatgctcgcg cttgagggcg
     1321 tgcgaagcgg ctacggcaag ctcgagatcg tccaggccgc gtcgctcgag gtcggggacg
     1381 gcgcgatcgt cggcatcatc gggcccaacg gggcgggcaa gtccaccctg ctcaagaccg
     1441 ccttcggcta tctgccgccc ttcgcgggcc gcatcgcgct cgacgggcgg gagatcgcgg
     1501 ggcgccggcc cgacgagatc atgcgcctgg gcgtcggcta cctggcccag gcgggcgggc
     1561 tcttcgcgga gatgaccgtg cacgacaacc tcgtgctcgg cggctacacc gtctcgcgcg
     1621 ccgacaagcg ccgcgccctc gatgcggtgt acgcgcgctt cccgctcttc cgggatcggg
     1681 gccgccagct ggccggcgcg ctgtccgggg gcgagcagcg cctcctcgcc atcgcccgcg
     1741 ccctcatcgt ccgcccccgc ctcctgctcc tcgacgagcc gtccgctgcg ctggccccgc
     1801 gcttcatcga cctgatctac gcgacgctca cggccctcaa tcgcgacggc ctcgccatgc
     1861 tgatcgtgga gcagaacgtg gaggcgatcc tggcggtggc ccaccgggtg ttcgtgctcg
     1921 acctgggccg caacgccttc gacgggacgc cggccgagct gcgggcctcc gaccggatcc
     1981 gccggctcta cctgggcgaa gaggggggcg aggacgagcc ggccgcgggg cccgtgcgct
     2041 gatgctcgag tccctcctcg agttcctgat ccagggcgtg gtcctcggcg gtctctacgc
     2101 gctcttcgcg gtcgggctca gcctgatctt cggcgtgctc gacgtcatca acgtggcgca
     2161 cggggagttc ttcgcggtcg gcggctacct ggccttcgcg gcggtggtgc tgctgcacct
     2221 gcccggcgcg gccggcgtgc tgggggccgc ggtcgggacc ttcctgctcg gcctctgcgt
     2281 ctaccctctc ctgatcgcgc cactcaagcg gcggctcgga ggccggccgc aggggccgct
     2341 ctacctcgtg ctcaccctcg ggctctccac gttcctccag tcgagcctgc tcgcgatcgc
     2401 gggaggcgac tacctgcgcg tcccgcccca ggtgagcggc atcctcgacc tgggcgtcac
     2461 cgcggtgagc caccagcgcc tgctcgtgct gggcgtcgcg gccgcgctgc tcaccgccct
     2521 cttcgcgttc ctgcgcctcc acccggtggg actcgcggtg cgcgcggtgg cgctcaaccc
     2581 ggacgcggcc caggcgatgg gcatcaacct gccgcggatc ttctcgctca ccctggccct
     2641 cggcgtcgcg ctggcggggg tgggcggcgc gctcatggct ccgctcttca acgtctaccc
     2701 ggcggtgggc ttcccgctca ccatcaaggc cttcgccatc accatcctcg gcgggatggg
     2761 caacccggtg ggcgcgctgg tggcgagctt tctcatcagc atcgcggagt cgctctcggt
     2821 gatggtgatc ccctcgcagt ggcagaacct catcgcgttc gcggtgatga tcgtgatgct
     2881 cctggtccgg ccccgcggcc tcttcggccg ccccgtgccg cgatgagtcg ccgcgcgctt
     2941 gccctcgtcg ccgccgccgg cgtcgcgctc ctgcccgtgc tgctgccgca cccgttcgtg
     3001 ctcaccatcg cgacgcaggc ggcgatctgg gccctgctcg ccgcgagctg ggatctcctg
     3061 agcggctaca cggggcagat ctcgttcggt cacgccggct tcttcgcact cggggcctac
     3121 gccgcggccg ccgcgaccaa gcacgcaggc gtctcgccgt ggctcggcct gctcgtcggc
     3181 gcgacgatcg ccgccgccgt c
//
LOCUS       DATMCV010000384         4568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_487122, whole genome shotgun sequence.
ACCESSION   DATMCV010000384 DATMCV010000000
VERSION     DATMCV010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487122"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VNU03_08915"
     CDS             <1..1247
                     /locus_tag="VNU03_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP74624.1"
                     /translation="VVGILWFAGASLACGIAPNAPALIVARALQGIGAALLTPGSLAI
                     IETTFHPEDRSAAIGAWSGLGGVMTAIGPVVGGYLTTVISWRLIFFINLPLAAVAAWA
                     ALRHLPESREAHVAGRLDYLGAALAALGLGGVVFAVTAGPVDGWATPPVLATGVGGII
                     ALIGFVVVERSRRHPLVPLEMFRVRQFTAANAVTFVVYGALGGALFLLPIQLQRVVGL
                     SPLESGSALIPMTVAMLLLSSFAGRLAQRAGPRRPMTVGPFVAAIGLALLVRVVPGGG
                     YWLTVFPGVVVFGLGLSITVAPLTATVLAAAGPERAGIAAAVNNDVARAAGLVAVAIL
                     PLVAGITGGGDLDPQILSAGFRTAMWVTASLAAAGGALSYLTIRDDAVAAGTSATAAP
                     DLAHHLSCPLDAPPLLGMQGRR"
     gene            1330..1842
                     /locus_tag="VNU03_08920"
     CDS             1330..1842
                     /locus_tag="VNU03_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP74625.1"
                     /translation="MPKLPKITPFLWFDTEAEEAANFYVSIFKNSRIKGLARYDGDTA
                     QAAGRPKGSVMTVQFELDGQDFVALNGGPTFKFTEAISLVVNCETQAEIDHFWQKLSA
                     GGQEVECGWLKDRFGLSWQVVPADIEEMLQDKDLEKPKRVMAAIMKMKKLDMAEMRRA
                     YEGRPEPSRS"
     gene            1885..3942
                     /locus_tag="VNU03_08925"
     CDS             1885..3942
                     /locus_tag="VNU03_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP74626.1"
                     /translation="MQIGVDIGGTFTDIVALDRTGRLALTKVPSTPKDLLDGIATATT
                     RVLALAGAAPGDVERFIHGTTVATNAVLEQKGATTAILTTEGFEDVLEMGRMKRSRMY
                     DLDMDPEVPTFIAPRRRRLGIRERLDAKGRVLVPLHEADVRRAVEVLRAQGVSAVAVC
                     YLFSFLNGAHERRTRDILAELAPDISVSLSSEVDPTFREYERSCVTAFDAYLGPVVKR
                     YLAGLTDTLRGLGVPGVPLIMRSRGGIVSAALAAQQPVTLFLSGPAGGVIGAAFAAER
                     SEVRDFVSLDMGGTSNDVALVRDGRPLLVGEGTIGSYPVRTPMVDVNTIGAGGGSIAW
                     IDAAGGLRVGPRSAGAEPGPACYARGGDEATVTDASLVLGYLNPARFAGGALTLDVAA
                     GERAVGTLGHRLGLDPVAAAAGIHRVVNARMADQIRLVTIKRGYDPRQFALVVLGGAG
                     PVHGAPLAEELGLAEVVVPEAPGVLAAFGLLAAAIEHHHARTLQTRTDAADLGAINAC
                     LGELDAAGRARMDEEGAPAPAVRVAYAADMRYVGQAYELEVPIPAPVTPPSVPAIVSA
                     FHAVHERVYGYARAQQPVEFVNFRAVHTFPLPRPAVTPAARATGTLAEAQVGERRAYF
                     GRFVPTAIYERDRLPLGARLAGPAIVEQTDTTTVIPPGVTALVDPVGNLRLRRDPPSG
                     GHP"
     gene            3939..>4568
                     /locus_tag="VNU03_08930"
     CDS             3939..>4568
                     /locus_tag="VNU03_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP74627.1"
                     /translation="MTAIDPILLEVLRNRLDAIADEMELTLLKSAASPIVKEGLDASA
                     ALFNTRGETIAQAAAIPIHLGALQFAAQRIVRAFPPEAMREGDAFLLNDPYDGGTHLP
                     DITLAVPVFADGAAVALACTMCHHQDVGGRTPGSVPTDATELYQEGVIIPPTQLFRAG
                     VLDDNLFRLLTRNVRLPEVFTGDLMAQVAAGRLGGVRLRELFAAHGAPTV"
BASE COUNT          597 a         1702 c         1592 g          677 t
ORIGIN      
        1 tcgtggtcgg cattctctgg ttcgccggcg cgtccctggc ctgtggcatc gcccccaatg
       61 ccccagccct gatcgtggcc agggcgctcc aggggatcgg cgccgccctg ctcacgccgg
      121 gaagcctcgc gatcatcgag accaccttcc acccggagga ccgctcggcc gcgatcggcg
      181 cctggtcggg cctcggcggc gtgatgaccg cgatcggccc cgtggttggc gggtatctga
      241 cgacggtcat cagctggcgg ctcatcttct tcatcaacct gcccctggcg gcggtggcgg
      301 catgggccgc cctgcggcat ctgcccgaga gccgggaggc gcacgtggcg ggccgtctcg
      361 actatctcgg ggccgcgctc gccgccctcg gcctgggcgg ggtcgtcttc gcggtcaccg
      421 ccggtcccgt tgacggctgg gccacgcctc cggtgctcgc gaccggagtc ggcgggatca
      481 tcgcgctcat cggcttcgtc gtcgtggagc gttcccgccg tcatccgctg gtgccgctcg
      541 agatgttcag ggtccgccag ttcaccgcgg cgaacgcggt cacgttcgtc gtctatggcg
      601 ccctgggcgg ggcgctgttt ctgctcccga tccagctgca gcgcgtggtc ggcttgtccc
      661 ctctggagtc cggcagcgcg ctgatcccca tgacggtcgc gatgttgctc ctctcgtcgt
      721 tcgccggtcg gctggcgcag cgagccgggc cgcgccggcc gatgacggtc gggcccttcg
      781 tggcggccat cggcctggcg ctgctggtgc gcgtcgtgcc cgggggcggc tactggctca
      841 ccgtcttccc gggcgtggtc gtcttcggtc tgggcctctc gatcacggtc gctccgctga
      901 ccgcaaccgt gctggccgcc gccggccccg agcgcgccgg catcgcggcg gcggtcaaca
      961 acgacgtggc gcgggcggca ggtctcgtcg cggtcgcgat cctgccgctg gtcgccggca
     1021 tcacgggtgg cggcgacctc gaccctcaaa tcctctccgc cggctttcgt accgcgatgt
     1081 gggtgacggc ctcgctggcg gccgccggcg gggcgctcag ctacctcacg atccgtgacg
     1141 atgcggttgc ggcgggcacg tccgccactg cggcgcccga cctcgcccac catctcagct
     1201 gtccgctgga cgccccgccc ttgctcggca tgcaagggcg gagatgaccg cgcgacaatg
     1261 accgctgtcg atcgagccgg tggccgttcg accaaccagt agacgaacat tccgacccag
     1321 gagggatcga tgcccaagct gccgaagatc acgccgtttc tctggttcga taccgaggcc
     1381 gaagaggccg cgaatttcta cgtctcgatc ttcaagaact cccggatcaa gggcctggcc
     1441 cgctacgacg gcgatacggc acaggccgcc gggagaccga agggctcggt gatgaccgtc
     1501 cagttcgagc tcgacgggca ggacttcgtc gccctgaacg gcggccccac cttcaagttc
     1561 accgaggcga tctccctcgt ggtgaactgc gagacgcagg ccgagatcga ccacttctgg
     1621 cagaagctgt ccgccggcgg ccaggaggtc gaatgcgggt ggctgaagga ccggttcggc
     1681 ctctcgtggc aggtcgttcc ggccgacata gaagagatgc tccaggacaa ggacctggag
     1741 aagccgaagc gggtcatggc ggcgatcatg aagatgaaga agctcgacat ggccgagatg
     1801 cgccgggcct acgagggcag gccggagccg tcgcgcagct gagcccacgt atgtgttaac
     1861 gttggcgccc tcgggagggc gcgcgtgcag atcggcgtgg acatcggcgg caccttcacg
     1921 gacatcgtgg ccctggaccg caccggccgg ctcgcgctga ccaaggtgcc gagcacgccc
     1981 aaggacctgc tcgacggcat cgccaccgcc accacccggg tgctggcgct cgccggcgcc
     2041 gcgccgggcg acgtcgagcg gttcatccac ggcaccaccg tggccacgaa cgcggtcctc
     2101 gagcagaagg gggccacgac cgccatcctg accaccgagg gcttcgagga cgtcctcgag
     2161 atggggcgga tgaagcgctc gcgcatgtac gacctcgaca tggacccgga agtgcccacc
     2221 ttcatcgcgc cgcgccgccg ccgcctgggc atccgcgagc gcctcgacgc gaaggggcgc
     2281 gtgctggtcc cgctgcacga ggccgacgtg cggcgggcgg tggaggttct gcgcgcgcag
     2341 ggggtgtcgg ccgtcgcggt gtgctacctg ttctcgttcc tgaacggagc gcacgagcgg
     2401 cggacgcgcg acatcctcgc ggagctcgcg ccggacatca gcgtgtccct ctcttctgag
     2461 gttgacccaa ctttcagaga atacgaacga tcctgtgtga ccgccttcga cgcctacctc
     2521 ggcccggtgg tgaagcgcta cctcgcgggc ctcaccgaca ccctgcgcgg cctcggggtg
     2581 ccgggcgtgc cgctgatcat gcgctcgcgc ggcggcatcg tgtcggcggc gctcgccgcc
     2641 cagcagcccg tcacgctctt cctctccggg ccggccggcg gggtgatcgg tgccgcattc
     2701 gcggccgagc gctccgaggt gcgcgacttc gtctcgctcg acatgggggg caccagcaac
     2761 gacgtggccc tggtgcggga cggccggccc ctgctcgtcg gcgagggcac gatcggatcc
     2821 tacccggtgc gcaccccgat ggtggacgtc aacaccatcg gggccggcgg cggcagcatc
     2881 gcgtggatcg acgccgcggg aggcttgcgg gtggggccgc gcagcgcggg cgccgagccg
     2941 ggccccgcct gctacgcccg gggcggcgac gaggccaccg tgaccgacgc cagcctcgtg
     3001 ctcggctatc tcaatcccgc gcgcttcgcg ggcggcgccc tgaccctcga cgtggccgcc
     3061 ggcgagcgcg cggtgggcac cctcggccac cggctggggc tcgacccggt ggccgcagcg
     3121 gcgggcatcc atcgcgtggt caacgcccgc atggcggacc agatccgcct ggtgacgatc
     3181 aagcgaggct acgaccctcg acagttcgct ctggtcgtcc tcggcggggc cggccccgtg
     3241 cacggcgccc ccctggccga ggagctgggc ctggcggaag tggtcgtccc ggaggcgccc
     3301 ggggtgctcg ccgccttcgg cctgcttgcc gcggccatcg agcaccacca tgcgcgcacc
     3361 ctccagacgc gcaccgacgc ggccgacctg ggagccatca acgcctgcct cggcgagctc
     3421 gacgccgccg gccgcgcgcg catggacgag gaaggggcgc ccgcgccggc ggtgcgcgtg
     3481 gcctacgcgg ccgacatgcg gtacgtgggc caggcctacg agctggaggt accgatcccg
     3541 gccccggtca cgcctccgtc cgtacccgcc atcgtgtccg cgttccacgc cgtgcacgag
     3601 cgcgtgtacg gctatgcgcg cgcgcagcag ccggtggagt tcgtcaactt ccgcgcggtc
     3661 cacaccttcc cgctgccgcg gccggcggtg acgccggccg cgcgcgcgac cggcacgctg
     3721 gccgaggcgc aggtcggcga gcgtcgcgcg tacttcggcc ggttcgtgcc gaccgcgatc
     3781 tacgagcgcg atcgcctgcc gctcggcgcc cgcctcgccg gaccggccat cgtggagcag
     3841 accgacacca ccaccgtgat cccccccggc gtcaccgcgc tcgtggaccc ggtcggcaac
     3901 ctgcgcctca ggagagaccc cccgtccggg ggacacccat gaccgccatc gaccccatcc
     3961 tcctggaagt cctgcgcaac cgtctcgacg ccatcgccga cgagatggag ctgaccctgc
     4021 tgaagagcgc ggcctcgccg atcgtgaagg agggcctgga cgcctcggcc gcgctgttca
     4081 acacccgcgg cgagaccatc gcccaggcgg ccgccatccc gatccacctg ggcgccctgc
     4141 agttcgcggc gcagcgcatc gtccgcgcct ttcccccgga ggccatgcgc gagggcgacg
     4201 ccttcctgtt gaacgacccc tacgatggcg gcacccacct gcccgacatc accctcgcgg
     4261 tgccggtctt cgcggacggc gcggccgtcg cgctggcctg caccatgtgc caccaccagg
     4321 acgtgggcgg ccgcaccccg ggcagcgtgc cgaccgacgc caccgagctc taccaggaag
     4381 gcgtcatcat cccgcccacc cagctcttcc gcgccggggt gctggacgac aacctcttcc
     4441 gcctgctcac ccgcaacgtg cggctcccgg aggtgttcac cggcgacctc atggcccagg
     4501 tggcggcggg gcgactcggc ggcgtccgcc tgcgtgagct gttcgccgcc cacggcgccc
     4561 ccacggtg
//
LOCUS       DATMCV010000385         3794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_956213, whole genome shotgun sequence.
ACCESSION   DATMCV010000385 DATMCV010000000
VERSION     DATMCV010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956213"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="VNU03_08935"
     CDS             <1..1639
                     /locus_tag="VNU03_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP74628.1"
                     /translation="DTALDEAAVAASAHRLAAQGVEGLVISFIHAYREASHERRARAL
                     AETAAPAVPVTASHEVWPEAREYERTALAVMNAYVQPKVRRYLEGFEAALTTRAVPAV
                     PYVTKSNGGIMPVAAARSQTVSTLLSGPASGVIGAAYVATAAGLGNLITLDVGGTSAD
                     IAVVEDGRPRYSTAEHIGGIPVMMPVVGVTAIGAGGGSVAWVDEVGVPKVGPQSTGAS
                     PGPACYGRGGKDATLSDAFLVCGFLDPDRFLGGRMRLDRTLAEEAVQRFADRLGMSLH
                     EAAESAVRVAVSNMYAASTKILSRAGVDPRDFALVAFGGAGPLVGSLLARDVGIPTVF
                     VPRSPGTLCALGAITTDVLNDAVRTVHGRLDALDLAALAREQRALEAELGAWVDEHRI
                     EVESVAFRHGADMRYVGQSYEIEVPVEPEWLATDGRHRLLEAFHRAHERVFGHADPHA
                     PVEVVNLRAQLRGVRPRVPLVEVAAGTGARATGARRIWLDGRPAQAQVYERASLGRGD
                     RVIGPAIVEQADTTVLVPAGDAATVDRFGNLLIRREA"
     gene            1639..3642
                     /locus_tag="VNU03_08940"
     CDS             1639..3642
                     /locus_tag="VNU03_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP74629.1"
                     /translation="MRLDPLTLELLRNYLHGAVEDMAYAVERTAHTTFVKETADFTCG
                     LITPAGDFFAYPVELGVASMAGINYARTLEVVGPLEPGDVVITNDPYGSAAAATHLPD
                     LHLVRPIFWEGRLVGHGAGFLHCSDIGGMVPASISPRASEIFQEGLRLPPKKLFVRGE
                     PNRDLLDVILANCRIPEQNWGDLQALVAGLVTGERRVHALIRRFGVDTVEKGMDDLID
                     YTARKVEALIRRMPRGRYQFCDFIEDDVVSDIPIRLNVGMTVGDGEIHLDFTGSDVQV
                     GSALNVPTGGRAHPFMAIALFNYFITKDPGIPLNAGVLRPIRMTLPVGSVVNPQFPAA
                     CGVRYATVLRIYDAVLGALARALPAEIPAASGGQGCMVALALPDLEAGRRHVTVIEPM
                     IGGGGGRPGLDGIDGCDASLGFLKTTPVETLEVEVPAILIRRFHLVADSAGPGRWRGG
                     HAVRLDMQVFRPEGQVTARGMERLRFAPWGVAGGRAGATGRAVLNPGTSRERAVPKID
                     LLALEPGDVLSMRTPGGGGNGDPLERPIEAVAADVEAGLVTPAHARAAYGVVMADGAV
                     DPVATSALRAERRAAAGPLGPFDFGAARDAHERRWPPELQDALVALLMTLPAPYRAYV
                     RRALYSRITARTDHGPVTTDDLARLWAELAASSGLSAGKGGNP"
     gene            3639..>3794
                     /locus_tag="VNU03_08945"
     CDS             3639..>3794
                     /locus_tag="VNU03_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP74630.1"
                     /translation="MKNLAGKVAVVTGAASGIGLALAERFAAEGMKVVMADIEAGPLA
                     TAAAAVRQ"
BASE COUNT          483 a         1362 c         1442 g          507 t
ORIGIN      
        1 cgataccgcg ctggacgagg ccgccgtggc cgcgtccgcc caccggctgg ccgcgcaggg
       61 cgtcgagggg ctcgtgatca gcttcatcca cgcctaccgc gaggcgtccc acgagcgccg
      121 ggcgcgcgcc ctggccgaga ccgcggcgcc ggccgtgccg gtgaccgcct cgcacgaggt
      181 gtggccggaa gcgcgcgagt acgagcgcac cgcgctcgcg gtgatgaacg cctacgtgca
      241 gccgaaggtg cgccggtacc tcgagggctt cgaggcggcg ctgaccacgc gcgcggtgcc
      301 cgcggtcccc tacgtgacca agtccaacgg cggcatcatg ccggtggcgg cggcccggtc
      361 gcagacggtg tcgacgctcc tgtcggggcc cgcgtccggg gtcatcgggg cggcttacgt
      421 cgccaccgcg gccggcctcg gcaacctgat cacgctcgac gtgggcggca cgagcgcgga
      481 tatcgcggtg gtggaggacg gacgcccgcg ctacagcacc gcggagcaca tcgggggcat
      541 cccggtgatg atgccggtgg tgggggtcac cgccatcggg gccgggggcg gctccgtcgc
      601 ctgggtcgac gaggtcggag tgcccaaggt cggtccccag agcacgggcg ccagtccggg
      661 cccggcgtgc tacggccgcg gcggcaagga cgccacgctc tccgacgcct tcctggtgtg
      721 cggcttcctc gaccccgacc ggttcctggg cggacggatg cggctcgacc gcacgctcgc
      781 cgaggaggcg gtccagcgct tcgcggaccg gctcggcatg agcctgcacg aggcggcgga
      841 gagcgcggtg cgggtcgcgg tgtcgaacat gtacgcggct tccaccaaga tcctctcgcg
      901 cgccggcgtc gacccgcgcg acttcgcgct ggtcgccttc ggtggcgcgg gaccgctggt
      961 cgggagcctc ctcgcccgcg acgtgggcat ccccaccgtc ttcgtgccgc gatcgcccgg
     1021 gacactctgc gccctcggcg ccatcaccac cgacgtcctg aacgacgcgg tgcggacggt
     1081 gcacggccgc ctcgacgcgc tcgatctggc cgcgctcgcc cgcgagcagc gggcgctcga
     1141 ggccgagctc ggcgcctggg tcgacgagca ccgcatcgag gtggagagcg tggcgttccg
     1201 ccacggcgcg gacatgcgct acgtcggcca gtcctacgag atcgaggtgc ccgtcgagcc
     1261 ggagtggctc gcgacggacg gacgtcaccg gttgctcgag gcctttcacc gggcgcacga
     1321 gcgggtgttc ggccacgccg acccccacgc gccggtcgag gtggtgaacc tccgggccca
     1381 gctccgcggg gtgcggccgc gcgtcccgct ggtcgaggtg gccgccggca ccggggcgag
     1441 ggcgaccgga gcccggcgga tctggctcga cggccggccg gcgcaggccc aggtgtacga
     1501 gcgcgcgagc ctgggccggg gcgaccgtgt gatcgggccg gccatcgtgg agcaggcgga
     1561 caccaccgtg ctcgtcccgg ccggcgacgc cgcgacggtc gatcggttcg gcaatctcct
     1621 catccgacgg gaggcgtgat gcgtctcgat cccctgactc tcgagctgct ccgaaactac
     1681 ctgcacggcg cggtggagga catggcctac gcggtggagc gcaccgcgca caccacgttc
     1741 gtgaaggaga ccgcggactt cacgtgcggc ctcatcactc cggccgggga cttcttcgcc
     1801 tatccggtcg agctcggcgt ggcgagcatg gccgggatca actacgcgcg gaccctcgag
     1861 gtggtgggtc cgctcgagcc gggcgacgtg gtgatcacca acgatcccta cggctccgcg
     1921 gccgccgcca cccatctccc cgacctgcac ctggtgcgcc cgatcttctg ggaggggcgg
     1981 ctcgtgggcc acggcgccgg cttcctccac tgctcggaca tcggcgggat ggtgccggcc
     2041 agcatctcgc cccgggcgag cgagatcttc caggaggggc tccgcctgcc gccgaagaag
     2101 ctattcgtgc gcggagagcc gaaccgggac ctcctcgacg tgatcctggc caactgtcgc
     2161 atccccgagc agaactgggg cgacctccag gccctggtgg ccggcctggt gactggcgag
     2221 cgccgcgtgc acgcgctgat cagacgcttc ggcgtcgata cggtcgagaa gggcatggac
     2281 gatctcatcg actacaccgc gcggaaggtc gaggcgctga tccggcggat gccccgcggc
     2341 cgctaccagt tctgcgactt catcgaggac gacgtggtgt cggacatccc gatccgcctc
     2401 aacgtcggga tgacggtggg cgacggcgag atccacctcg acttcaccgg cagcgacgtg
     2461 caggtgggct ccgccctcaa cgtcccgacc gggggccgcg cgcatccgtt catggcgatc
     2521 gcgctgttca actatttcat caccaaggat cccggcatcc cgctgaacgc gggcgtgcta
     2581 cggccgatcc ggatgacgct gccggtcggg tcggtggtaa acccgcagtt ccccgcggcg
     2641 tgcggcgtcc gctacgccac cgtgctgcgc atctacgacg cggtgctggg cgcgctggcc
     2701 cgcgcgctgc ccgcggagat cccggccgcg agcggcgggc agggctgcat ggtcgccctc
     2761 gcgctgccgg acctcgaggc ggggcggcgc cacgtgaccg tgatcgagcc gatgatcggt
     2821 ggcgggggcg gtcgccccgg gctcgacggg atcgacggct gcgacgcctc gctcggcttc
     2881 ctcaagacga ccccggtgga gaccctcgag gtcgaggtgc ccgcgatcct gatccgccgc
     2941 ttccacctcg tcgccgactc cgccgggccc gggcgctggc gcggcgggca cgcggtacgg
     3001 ctcgacatgc aggtgttccg gcccgaggga caggtcaccg cgcgagggat ggagcggctc
     3061 cgcttcgcgc cctggggggt ggcgggaggc cgcgcgggcg ccaccggccg ggccgtgctc
     3121 aaccccggca cgtcgcgcga gcgcgcggtg cccaagatcg atctgctcgc gctggagccg
     3181 ggcgacgtgc tgagcatgcg gacgcccggc ggcggcggga acggtgatcc cctggagcgg
     3241 ccgatcgagg cggtagccgc cgacgtggag gccggcctcg tgacgccggc gcacgcgcgc
     3301 gcggcgtacg gcgtggtgat ggcggacggc gccgtcgacc cggtggcgac gagcgcgctg
     3361 cgggcggagc gccgtgccgc cgccggcccg ctcggcccgt tcgacttcgg cgcggcccgc
     3421 gacgcccacg agcggcgctg gccgcccgag ctgcaggacg cgctcgtcgc gctgctcatg
     3481 accttgcccg cgccgtaccg ggcgtacgtc cgccgggcgc tctattcgcg gatcacggcg
     3541 cggacggacc acggaccggt gacgacggac gatctggccc gcctctgggc ggagctcgcc
     3601 gcgtcgtccg gcctgtcggc gggcaaggga gggaacccgt gaagaatctc gccgggaagg
     3661 tcgcggtggt caccggcgcg gcgagcggga tcgggctcgc gctcgcggag cgcttcgcgg
     3721 ccgagggcat gaaggtggtg atggccgaca tcgaggccgg gccgctcgcg accgcggcgg
     3781 cggcggtgcg acag
//
LOCUS       DATMCV010000386         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_354571, whole genome shotgun sequence.
ACCESSION   DATMCV010000386 DATMCV010000000
VERSION     DATMCV010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354571"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="VNU03_08950"
     CDS             <1..2047
                     /locus_tag="VNU03_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWP74631.1"
                     /translation="RANPHVLVLATSREPLRAPGEYVYRLLSLQVPAEDTDGHEDLLE
                     AAAVKLFLARAQAMDRRFFLDARTAARTATLGIEALAARLDDRFRLLTGGHRTALRRH
                     QTLHATLDWSYDLLPPVERTVLHRLAVFAGGFTLQAASAVATGPDLGGPEVMDSVANL
                     VAKSLVVVEVADAVTRYRLLETMRAYALEKLTERGELDQVARRHAEFYRDLLERSETE
                     RETRSTVEWVALYGRQIDNLRTALDWAFSPRGDATLGVALTAAAVPLWFQRSLMVECR
                     GRAEHALGSLGPRPGRDARREMQLCAALGVSLMQTKGPAPDTIAAWTTALEIAERLAD
                     TEYQLRALWGLWHFRVSRGECRAALDLAERFSALVARTANPADRPVGERMVGASLHYL
                     GDQPNARRHLESMLDHYVASARRSHAIRFQYDQPLVARMILARILWLQGFPDQGLRLA
                     HANVEDAKAIGHGLSLCGALEVACLVAIWSGELADAERSVAMLLDQSGHHALSVWHTR
                     GQCLNGVVLIRRGDVGPGFEVLRSALDELRETDFVPYYTGLLGTLAQGLVGVGQVAEG
                     LTTIDEALARSERDEEHWCIAELLRIKADLLLLAGGPGAAAAAEGQFQQALDWTRRQA
                     ILSMELRCAVGLAGLWHDQGRTGPARELLAPVYNRFTEGFDTAELQAANALLDRLRQ"
     gene            complement(2118..2381)
                     /locus_tag="VNU03_08955"
     CDS             complement(2118..2381)
                     /locus_tag="VNU03_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74632.1"
                     /translation="MIVCDSDTLSIDARWLSGRSSATAAVASLSTGTLATHAKDAIEA
                     ALTESKGRVAGPFGAAGRLGVPASTLESKIKALNIHKRRFKSG"
     gene            complement(2368..2721)
                     /locus_tag="VNU03_08960"
     CDS             complement(2368..2721)
                     /locus_tag="VNU03_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWP74633.1"
                     /translation="MLLRVLQEREFERVGGSGPVRVNVRVIAATNRDLHAAVADGTFR
                     ADLFYRLNVFPLDVPALRRCPPARRILHPSLRQAPRQADPPRHQGDVAAASVVRLAGQ
                     YPRAAERHRARGDRV"
BASE COUNT          381 a          998 c          967 g          402 t
ORIGIN      
        1 gcgcgccaat ccccacgtcc tggtgctggc gaccagccgc gagccgctgc gcgcccccgg
       61 cgaatacgtc taccggctac tctcgctcca ggtgccggcc gaggacaccg atgggcacga
      121 ggatctgctg gaggcggccg cggtcaagct gttcctggct cgcgcccagg cgatggaccg
      181 gcgcttcttc ctggacgccc gcaccgccgc ccgcaccgcc acgctcggca tcgaagcgct
      241 cgcggcgcgg ctggacgatc gcttccggct gctcaccggt ggccatcgga ccgcgctgcg
      301 gcgacatcag acgctgcacg caactctcga ctggagctac gacctgctgc ccccggtcga
      361 gcgcaccgtc ctgcaccggc tcgcggtctt cgcgggcggc ttcacgctgc aggcggcgag
      421 cgcggtcgcg accggccccg acctcggcgg gcccgaggtc atggacagcg tcgcgaacct
      481 ggtcgcgaag tcgctcgtgg tcgtggaggt cgccgacgcg gtcacccgat atcgcctcct
      541 cgagacgatg cgcgcctatg cgctcgagaa gctcaccgag cggggcgagc tcgaccaggt
      601 ggcccgccgc cacgccgaat tctaccggga cctgctcgag cggtcggaga ccgagcgcga
      661 gacgcgctcc accgtcgagt gggtggccct ctacggccgc cagatcgaca acctgcggac
      721 cgcgctggac tgggccttct ccccgcgcgg cgacgcgacg ctcggagtgg cgctcaccgc
      781 cgccgcggtc ccgctctggt tccagcggtc gctcatggtt gagtgccgcg gccgcgccga
      841 gcacgcgctg ggaagcctcg ggccgcgccc ggggcgcgac gcgcggcggg agatgcagct
      901 ctgcgccgcg ctcggggtgt cgctcatgca gacgaagggg ccggctcccg ataccatcgc
      961 ggcgtggacc accgcgctcg agatcgcgga gcgcctcgcc gacaccgagt accagctccg
     1021 cgccctctgg ggtctctggc atttccgcgt cagccgcggc gagtgtcgcg cggcgctgga
     1081 cctggcggag cgcttcagcg cgctggtggc ccgcaccgcc aatccggccg accggccggt
     1141 cggcgagcgg atggtcggcg cctcgctcca ttacctcggg gaccagccga atgcgcggcg
     1201 acatctcgag agcatgctcg atcactacgt cgcctccgct cggcgatccc acgcgatccg
     1261 ctttcagtac gaccagccgc tggtggcgcg catgatcctg gcccggatcc tctggctgca
     1321 ggggttcccc gaccagggcc tgcggctcgc ccacgccaac gtcgaggacg ccaaggcgat
     1381 cggccacggg ctctcgctgt gcggcgcgct ggaggtcgcg tgcctggtcg cgatctggag
     1441 cggggagctg gccgacgccg agcgctcggt ggcgatgctg ctcgaccagt cggggcacca
     1501 cgcgctgtcc gtctggcaca cgcggggcca gtgcctgaac ggcgtcgtgc tgatcaggcg
     1561 cggagacgtc ggccccggat tcgaggtcct gcgctcggcc ctcgacgagc tccgcgagac
     1621 ggacttcgtt ccctactaca ccggcctgct cggcacgctg gcccagggcc tcgtcggggt
     1681 cggtcaggtc gccgaggggc tcacgacgat cgacgaggcg ctcgcccgat ccgagcgcga
     1741 cgaggaacac tggtgcatcg cggagctcct gcgcatcaag gccgacctcc ttctgctggc
     1801 ggggggcccg ggcgccgccg cggcggccga ggggcagttt cagcaggcgc tcgactggac
     1861 gcggcggcaa gcgatcctct ccatggagct gcgctgcgcc gtcggcctcg ccgggctgtg
     1921 gcacgatcag ggccggaccg ggccggcgcg cgagctgctg gcgccggtct acaaccggtt
     1981 caccgaaggc ttcgacaccg ccgagctcca ggccgcgaac gccctgctcg accggctccg
     2041 ccaatagcat tgaaggtctg ccaggacggc gttcctgcgc gtcgggctag cgcagctcgg
     2101 ccaggcgctt tctgcagcta ccccgacttg aagcgccgct tgtggatgtt cagcgccttg
     2161 atctttgact ccagcgtcga cgccggcacc ccgagccgcc ctgcggcgcc gaagggcccg
     2221 gccacgcgac ccttactttc ggtgagcgcc gcctcgatgg cgtcctttgc gtgggtcgcc
     2281 agcgtcccgg tcgagaggct ggcgaccgcc gcggtcgcag aggaccgacc ggagagccac
     2341 cgggcatcga tcgagagcgt gtccgaatca cacacgatca ccgcgcgctc gatgacgttc
     2401 tgcagctcgc ggatattgcc cggccagtcg tacgactgaa gcagctgcga cgtctccttg
     2461 gtgacgcggc ggatccgctt gccgaggcgc ctggcgtagc gatgggtgaa gtattcgacg
     2521 agcaggcgga catcggcgca gggcaggcac gtccaagggg aacacgttga gccgatagaa
     2581 gaggtccgcg cggaaggtgc cgtcggccac cgcggcgtgc agatcgcgat tcgtcgcggc
     2641 gatcacacgc acgttcacgc ggacgggccc gctgccaccg acccgctcga attcgcgctc
     2701 ttgcaacacg cgcagcagca tgatctgagt ctcggcgggg agttcgcc
//
LOCUS       DATMCV010000387         4667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_220263, whole genome shotgun sequence.
ACCESSION   DATMCV010000387 DATMCV010000000
VERSION     DATMCV010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220263"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2304)
                     /locus_tag="VNU03_08965"
     CDS             complement(<1..2304)
                     /locus_tag="VNU03_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HWP74634.1"
                     /translation="MRIAIGCDDTGFPLKEPLVAALEAEGHDVLDLGTFSTDPVDYPD
                     YARAVGQAVLRGFVDASLLLCGSGAGGVIAANKMRGIRAALCHDAAAARTSREEADAN
                     VLCLAAPGLEAKDAIEIAQTFIATQYEPSEQQARHVTKIGQLEAGLLGADKAPIKPGA
                     ADAKAAPTPKAAAPKGASKPARREAAAATAPRAAAAPERPAPPPAVVNPPPVLPDPLK
                     LPAVEETLRFLESREFLDRLWVKDATLWKGDAATVRNRLGWLTSPTIMRGHSEDIRGF
                     ADEVRRLQYSHVVLLGMGGSSSTGAVFNETFGSKMGFPDMLVLDSTDPGAVKQVADGV
                     NLSRALFVVSSKSGTTSETMAAYAYFRGQVEAASSPRPGMQFVAITDPGRPLDKLATE
                     TGFRRTFLNPASIGGRFSALSFFGLVPAAMIGVDIKILLERAHGMVETCGNEVGVRGN
                     PAVQLGAVLAGLARAGRDKATLVFSEKIRSLGPWIEQLLAESLGKDGTGIVPVVDEPL
                     GAPTVYGDDRVFVALVLEGDDSHDAALLKLADAGHPVLRLVLRDPMDLGAEFFRWELA
                     AATAGAVLGVNPFDEPDVARAKENTTTLLTEWRRSHRLPEWPTDVEEDGIVLMSKSNK
                     KPSSVSRGLAAHLAMAAPGDYLAIQAYLTPTAEAWRVLQEIRVALRDRLRIATTVGWG
                     PSHLHSTGQMHKGGPTSGLFVQITGDDREDLAIPGAGYGFSTLKAAQALGDLQSLRDA
                     ARRVIRLHLNGKQSQGLQQLLQMVQGLA"
     gene            complement(2327..3796)
                     /locus_tag="VNU03_08970"
     CDS             complement(2327..3796)
                     /locus_tag="VNU03_08970"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HWP74635.1"
                     /translation="MLVVALDVGTSSARARVFDATGRAQAGAEGHVAYEPTTSADGGV
                     ELHPGALMEAVLGALDRCLAGVGARAGDVAAVGVSVFWHSLLALDGDGRPLTPVITWA
                     DTRSAAAALALRREHDERAIHARTGAPLHSAFFPAKLRWLRQARPEVFRHAATWCGFG
                     EYLQAQLTGLMSASLSMASGTGLLDQASGRWDEAMLEAAGIAAATLPRIDDTAAPGLR
                     APWAARWPALARVPWHPARGDGACSNVGSDCWGPGRIALNVGTSAALRVVLPEPLGPH
                     AATPWGLWRYRIDARRALVGGATSEGGNVPAWCRKVLALPAHEDALEQAIAEVPPDGH
                     RLTALPFFAGERSPGWQPEARAAVAGLSLDTRAVEITRALLEAVAFRLAEIYDRLRPM
                     AAVDHVVVGSGGALGHSAAWAQIITDALGVPIALGLDAEASARGAALLALEALGQPPP
                     PAPAPARVLQPDPARHARYRAARARQSRLYDNIVGYRDS"
     gene            3817..4050
                     /locus_tag="VNU03_08975"
     CDS             3817..4050
                     /locus_tag="VNU03_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74636.1"
                     /translation="MEASPARGAEPEWELLTLRGLAMTDETASEFTGTLVIHRRGSPE
                     PVENVSVRIKRGMFEEMAGAMQRVLARSTPFTR"
     gene            4068..4355
                     /locus_tag="VNU03_08980"
     CDS             4068..4355
                     /locus_tag="VNU03_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74637.1"
                     /translation="MSLITVLSPVAELRAATTAIPRLPADLRGLTVGFLDNTKHNFDR
                     LVEGIGLILKERHGVERVVIRRKANAATGAAHEIIEALAKECDLVFAGSAD"
     gene            4437..4610
                     /locus_tag="VNU03_08985"
     CDS             4437..4610
                     /locus_tag="VNU03_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74638.1"
                     /translation="MFQRLALGEMAALGATGLPLLVIQHPLGGEAPEGVSRRTVQAVE
                     QLAAMLGGASAPR"
BASE COUNT          632 a         1656 c         1770 g          609 t
ORIGIN      
        1 ggccagtccc tggaccatct gcagcagctg ctgcaggccc tgcgactgct tgccgttcag
       61 atggagccgg atgacccggc gcgcggcgtc gcgcagactc tggagatcgc ccagcgcctg
      121 ggcggccttc aacgtggaga atccgtagcc cgcgccgggg atggcgagat cctcgcggtc
      181 gtcgccggtg atctggacga agagcccgct ggtggggcca cccttgtgca tctgcccggt
      241 cgagtgcagg tggctcgggc cccagcccac cgtggtcgcg atgcggaggc gatcgcgcag
      301 ggccacccgg atctcctgca gcacccgcca ggcctccgcg gtcggggtga ggtaggcctg
      361 gatcgcgagg tagtcgcccg gggcggccat cgcgagatgg gccgcgaggc cacggctcac
      421 cgagctcggc ttcttgttgg acttgctcat gagcacgatg ccgtcttcct cgacgtcggt
      481 cggccactcg ggcagacggt gggagcgccg ccactcggtg agcagcgtcg tggtgttctc
      541 cttggcgcgc gcgacgtcgg gctcgtcgaa gggattcacc cccagcaccg cgcccgcggt
      601 ggccgcggcc agctcccagc ggaagaactc cgcccccagg tccatggggt cgcgcaggac
      661 gaggcgcagc accgggtgtc ccgcgtcggc cagcttgagg agcgccgcgt cgtgcgagtc
      721 gtcgccctcg agcacgaggg ccacgaagac ccggtcgtca ccgtagacgg tcggggcgcc
      781 gagcggctcg tcgaccaccg ggacgatgcc ggtcccgtcc ttgccgagcg actcggcgag
      841 cagctgctcg atccaggggc cgagggagcg gatcttctcg gagaagacga gggtggcctt
      901 gtcgcgcccg gcccgggcga ggccggcgag caccgcgccg agctgcaccg cggggttgcc
      961 gcgcactccg acctcgttgc cgcacgtctc caccatgccg tgggcccgct cgagcaggat
     1021 cttgatgtcg accccgatca tcgcggcggg aaccagcccg aagaaggaca gggcggagaa
     1081 ccgcccgccg atcgacgcgg gattgaggaa ggtgcggcgg aagccggtct cggtggcgag
     1141 cttgtccagc ggccggcccg gatcggtgat ggccacgaac tgcatgccgg gccgcggcga
     1201 ggaggccgcc tcgacctggc cccggaagta ggcgtaggcg gccatggtct cgctggtggt
     1261 gcccgacttg ctcgagacca cgaagagcgc gcggctcagg ttgacgccgt cggccacctg
     1321 cttgaccgcg cccggatcgg tggagtcgag cacgagcatg tccgggaagc ccatcttcga
     1381 tccgaaggtc tcgttgaaga ccgcgccggt cgacgaggag ccgcccatgc cgagcaggac
     1441 cacgtgggag tactgcaggc gccgcacctc gtccgcgaag ccgcggatgt cctccgagtg
     1501 cccgcgcatg atggtgggcg aggtgagcca gcccagccgg ttccgcaccg tcgccgcgtc
     1561 gcccttccag agggtggcgt ccttcaccca gaggcgatcg aggaactcgc gcgactcgag
     1621 gaagcgcagg gtctcctcca ccgcgggcag cttgagcggg tccggcagca ccggcggcgg
     1681 attgaccacc gcgggcggcg gggccgggcg ctcgggcgcg gcggcggcgc gcggggcggt
     1741 ggccgccgcg gcctcgcggc gcgcgggctt ggaggctcct ttcggcgcgg cggccttcgg
     1801 ggtgggcgca gccttcgcgt ccgccgcgcc cggcttgatc ggggccttgt cggccccgag
     1861 cagccccgcc tcgagctgcc cgatcttggt cacgtgtcgc gcctgctgct cgctgggctc
     1921 gtactgggtg gcgatgaagg tctgggcgat ctcgatcgcg tccttggcct cgaggccggg
     1981 cgcggccagg cagaggacgt tggcgtccgc ctcctcacgg ctcgtccggg ccgccgccgc
     2041 gtcgtggcag agggcggcgc ggatgccacg catcttgttg gccgcgatca ccccgccggc
     2101 gccgctgccg cagaggagca ggctcgcgtc cacgaaacca cggaggacgg cctgcccgac
     2161 cgcccgggcg tagtccgggt agtccaccgg atccgtcgag aaggtgccga ggtcgagaac
     2221 atcgtgacct tcggcctcga gcgcggccac caggggttcc ttgagcggaa atccggtgtc
     2281 gtcgcagccg atcgcgattc gcatggtccc cgcgccgtct attctctcag gagtccctat
     2341 acccgacaat gttgtcgtag aggcgcgatt ggcgggcccg ggcggcccgg taccgggcgt
     2401 gccgagccgg gtcgggctgg aggacgcgcg ccggggccgg ggcgggcggt gggggctggc
     2461 ccagagcttc cagggcgagc agggcggcgc cccgggcgga ggcttccgcg tcgaggccaa
     2521 gcgcgatggg gaccccgagg gcgtcggtga tgatctgggc ccacgcggcg gagtggccca
     2581 gcgcgccgcc cgagcccacc accacgtgat cgaccgcggc catcgggcgc aggcggtcgt
     2641 agatctcggc caggcggaag gccaccgcct cgagcagcgc ccgcgtgatc tcgaccgcac
     2701 gcgtgtcgag cgagagcccg gcgaccgcgg cgcgggcctc cggctgccag cccgggctac
     2761 gctcaccggc gaagaacggc agcgcggtca accggtgccc gtccggcggt acctcggcga
     2821 tcgcctgctc gagcgcgtcc tcgtgcgcgg gcagggcgag caccttgcgg caccaggccg
     2881 gcacgttccc gccctcggag gtggcgccgc ccacgagcgc ccgcctggcg tcgatccggt
     2941 accgccagag cccccacggg gtggcggcgt gcgggccgag cggctcgggc aggaccacgc
     3001 gcagggcggc ggaggtgccc acgttgagcg cgatccgtcc ggggccccag caatccgagc
     3061 ccacgttgga gcaggcgccg tcgccgcgcg ccggatgcca cggcacccgc gcgagcgcgg
     3121 gccaccgggc ggcccacggc gcgcgcagcc cgggcgcggc ggtgtcgtcg attcggggaa
     3181 gcgtcgccgc ggcgatgccc gccgcctcca acatcgcctc gtcccaccgg ccgctcgcct
     3241 ggtcgagcag cccggtgccc gaggccatcg acaggctcgc gctcatgagg ccggtgagct
     3301 gcgcctgcag gtattcgccg aagccgcacc aggtggcggc gtgccggaag acctcgggcc
     3361 gcgcctgtcg cagccagcgg agcttggccg ggaagaacgc ggagtggagc ggggcgccgg
     3421 tgcgcgcgtg gatcgcgcgc tcgtcgtgct cgcggcgcag cgcgagcgcg gcggcggcgc
     3481 tgcgcgtgtc ggcccaggtg atgaccggcg tgagcggtcg gccgtctccg tccagcgcga
     3541 gcaggctgtg ccagaagacg gagacgccca ccgcggcgac gtcgcccgcg cgggcgccca
     3601 cgccggccag acagcgatcg agcgcgccca ggaccgcttc catcagtgcg ccgggatgaa
     3661 gctcgacgcc gccgtcggcc gaggtggtgg gctcgtaggc cacgtggcct tccgcgcccg
     3721 cctgcgcgcg gcccgtcgcg tcgaagaccc gggcccgggc ggagctggtg ccgacgtcga
     3781 gcgccaccac gagcatgtgc ggtagtatac gagcctatgg aggcgagccc ggcgcggggg
     3841 gcggagccgg agtgggaact gctgactctc cgcggcctgg ccatgaccga cgagaccgcc
     3901 tccgagttca ccggtacgct cgtgatccac cgccggggca gcccggagcc ggtcgagaac
     3961 gtgagcgtcc ggatcaagcg cggcatgttc gaggagatgg ccggggcgat gcagcgcgtg
     4021 ctggcccgct ccacgccgtt cacccgctga acaggagagc gctgaccatg tccctcatca
     4081 cggtgctgag cccggtggcc gagctccggg cggcgacgac cgcgattccg cggctgcccg
     4141 ccgacctccg gggcctcacg gtgggcttcc tcgacaacac caagcacaac ttcgaccgcc
     4201 tggtggaagg catcggcctg atcctcaaag agcgccacgg cgtcgagcgc gtcgtcatcc
     4261 gtcgcaaggc caacgcggcc accggggccg cgcacgagat catcgaggcc ctcgccaagg
     4321 agtgcgatct cgtcttcgcg ggctccgcgg actgaggctc ctgcacgtcg tggagtctcc
     4381 acgactcgct ggagctggcg aaggcgggaa cccccgcggg actgatcacc accaccgtgt
     4441 tccagcgcct cgcgctgggt gagatggccg cgctcggcgc gaccggcctc ccgctgctcg
     4501 tgatccagca cccgctaggg ggcgaggcgc cggagggcgt gagccgccgg acggtgcagg
     4561 cggtcgagca gctcgccgcg atgctcgggg gggcgtccgc cccccgatga gcgagacggt
     4621 cgacgccagc ctgaccctgc cggaagaaga cgtctacgcg gagttct
//
LOCUS       DATMCV010000388         4166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_423105, whole genome shotgun sequence.
ACCESSION   DATMCV010000388 DATMCV010000000
VERSION     DATMCV010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423105"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..2710
                     /locus_tag="VNU03_08990"
     CDS             68..2710
                     /locus_tag="VNU03_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74639.1"
                     /translation="MKKVLAAVLAVLVVLGAGLYFFVARPLLRPSEVTAVAERALATD
                     DLLLLAGINVKQAVFLEKWLLGSPRVTPVAATPPPALADRSLLDHLRAAGVDPRHDVD
                     HALYALYPADAAGSRHAVILVGRFNPAAVNAYLTRELAATPRPGPGPASFDVTRIDPA
                     TCQPGASWAVTVAREWILMADPISQPALIARLTGAPADTPERLAWWRGLAREDVASVG
                     IPGLERLESGATQPFVKGGAKALAGEATAFGRVYLGLGVKTVPPQGVLRIVVDAKDAA
                     RGAEKIRAWEQTVNASRDRWKESMPSVAKLYDSIRVQTVGARSTIEFTVDRTLAANSQ
                     RVIDEVLAAAFGGLGIRVNPPSAEAPAERIDTEPVVFLPAVTLAALPAYDPQAQFAEE
                     VDQIQGPFGVRLGELRLGAEPPAGSPAGSPAAGLEIMVEGFANEIPNVGASEDRVRLF
                     VDSVKAAGGQELLRPEACGRERNNQPVSFKSTGGKRLKATKAVRLVAGADPKALQSLT
                     GRVQLRLPTRTEVVTLEHPTPGATAERYGAVFTVTGIAPGRVSYQIAGAGSRLLLYRA
                     LNAKGQPLASPSAFSSDFMFGEGVSGQRQFAGTVDRLEIVFAAEEETVELPFTLTDFA
                     PAGKPSGLALDRTPAFRPYSVRELQREFPRPRGAGPLEPFELSLDRVQSFFAMRFDLT
                     VRSPAVPNFEHGFAAGQLRLTRVELKDGSVLTPPAAAPENPVAVRSRWESAVRFTGSP
                     KDGRLQTSLSLWVDTKAKPEDLKSLQGTLTLLYPRTLETLTLDDLTVGRQVQHGELTV
                     TVAARGRKSITLATSRDGDRIYYVRLLGADGQALAYFGPNITEGPGGAWRFDLSPLNP
                     PVKAEIVLAGQVERQTLPVSLTPR"
     gene            2758..3753
                     /locus_tag="VNU03_08995"
     CDS             2758..3753
                     /locus_tag="VNU03_08995"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWP74640.1"
                     /translation="MKLLVALLVGILLLAPAAAFAQAPLQLKAGSGVGPGNPVNDALF
                     DLAKSLDQKSGGRIKLDVVISNGLAKGEAAHLEGAQLGTIDIVPIGSAPIGGMFEAAY
                     QALDLPFFWSSREQVWKVMDGPIGQELLKRMEAKGVKGFCFGGGWGFRNMMVNKRPIM
                     VPDDMKGLTIRVQESPTYVAFMKALGANPVPMPYVEVYLAMKQGTIDGMELPSFTMTS
                     DKFQEVTKYYSLTRHSYPPIAYFMNMKRYQSLAPDLQKVVADSMQQACAAHRRFEVEK
                     EKQDLDVMKKAGTQVNEVKDLKAFQALMKPVYAGVEQQVGKEFMDRLTAAAQAAR"
     gene            3764..4054
                     /locus_tag="VNU03_09000"
     CDS             3764..4054
                     /locus_tag="VNU03_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HWP74641.1"
                     /translation="MPAYVIANVTVTDLPAMEEYRKQVPATLAKYGGRFLVRGGGHQT
                     VEGDWKPTRLVVIEFPTLAAAQRWYDSEEYRAPRALRMKAGRTDMVMVDGVA"
BASE COUNT          619 a         1485 c         1479 g          583 t
ORIGIN      
        1 gggctagacc tcctccccgg caacaaatcg ctgcggtccc gcgaagccgc tcccgtacca
       61 tcggcgcatg aagaaggtgc tggccgcggt gctcgcggtc ctggtggtgc tcggggctgg
      121 actctacttc ttcgtggccc gcccgctgct ccggccctcc gaggtgaccg cggtcgcgga
      181 gcgggcgctc gcgaccgacg acctcctgct gctggccggg atcaacgtca agcaggcggt
      241 cttcctcgag aaatggctcc tcggctcgcc gcgcgtcacc ccggtcgcgg ccacgccgcc
      301 ccccgcgctg gcggaccgct ccctgctcga tcacctccgc gcggcgggcg tggatccccg
      361 ccacgacgtc gaccacgcgc tctacgccct ctacccggcc gacgccgccg gctcgcgtca
      421 cgccgtgatc ctggtcggcc gcttcaaccc cgccgcggtc aacgcctatc tcacccgcga
      481 gctcgcggcg acgccgcggc ccggcccggg cccggcctcg ttcgacgtga cccggatcga
      541 tcccgccacc tgtcagcccg gcgcgagctg ggcggtgacg gtggcccggg agtggatcct
      601 gatggccgac ccgatctccc agccggccct catcgcccgc ctgaccggcg cgcccgccga
      661 cacgccggag cggctcgcct ggtggcgcgg cctcgcgcgc gaagacgtgg cgagcgtggg
      721 catcccgggc ctcgagcggc tcgagagtgg cgccacccag cccttcgtga agggcggggc
      781 caaggccctc gccggcgagg ccaccgcgtt cggccgggtc tacctggggc tcggcgtgaa
      841 gacggtgccg ccccagggtg tcctgcgcat cgtcgtcgac gccaaggacg cggcgcgcgg
      901 cgcggagaag atccgggcct gggagcagac ggtgaacgcg tcgcgcgacc gttggaagga
      961 gtcgatgccg agcgtggcga agctctacga cagcatccgg gtgcagaccg tgggcgcgcg
     1021 cagcacgatc gagttcacgg tcgaccgcac gctcgcggcc aattcgcagc gggtgatcga
     1081 cgaggtgctg gccgccgcct tcggcggcct cggcatccgc gtcaacccgc cgagcgcgga
     1141 ggcgcccgcc gagcggatcg acaccgagcc ggtcgtgttc ctccccgcgg tgaccctggc
     1201 cgcgctgccc gcctacgatc cgcaggccca gttcgccgag gaggtggacc agatccaggg
     1261 cccgttcggg gtgaggctcg gcgagctgcg cctgggcgcg gagccgccgg cgggatcgcc
     1321 cgccggatcg cccgcggcgg gcctggagat catggtcgag ggcttcgcca acgagatacc
     1381 gaacgtgggc gcgagcgagg accgcgtgcg cctgttcgtc gacagcgtga aggcggccgg
     1441 cggccaggag ctgctgcggc ccgaggcgtg cggccgggag cggaacaatc agccggtatc
     1501 gttcaagtcg acggggggca agcggctcaa ggccacgaag gcagtgcggc tcgtcgcggg
     1561 cgctgatccg aaggcgctgc agagcctgac cggccgggtc cagctccgcc tgcccacccg
     1621 gaccgaggtg gtgacgctgg agcatcctac tcccggcgcc accgccgagc ggtacggggc
     1681 ggtcttcacg gtcaccggga tcgcccccgg ccgcgtctcc taccagatcg cgggcgccgg
     1741 cagccggctg ctcctctatc gcgccctgaa cgccaagggg cagccgctgg cctcgccgag
     1801 cgcgttctcc tccgacttca tgttcggcga gggcgtgtcc gggcagcggc agttcgcggg
     1861 gacggtggac cggctcgaga tcgtgttcgc ggccgaggag gagacggtgg agctgccgtt
     1921 cacgctgacc gacttcgcgc cggccggcaa gccgagtggg ctcgccctcg accggacacc
     1981 cgcgttccga ccctacagcg ttcgggagct gcagcgcgag ttcccgcgac cgcgcggggc
     2041 cggcccgctc gagccgttcg agctctcgct cgatcgggtg caatcgttct tcgccatgcg
     2101 gttcgatctc acggtgcgga gcccggcggt tccgaacttc gagcacggct tcgcggccgg
     2161 ccagctccgc ctcacccggg tcgagctcaa ggacggcagc gtgctcaccc cgcccgcggc
     2221 ggccccggag aacccggtcg cggtgcggtc gagatgggag agcgcggtgc gcttcaccgg
     2281 ctcccccaag gacgggcgcc tccagacttc gctgagcctc tgggtcgaca ccaaggccaa
     2341 gcccgaagat ctgaagtcgc tgcagggaac gctcaccctg ctgtatcccc gcacgctcga
     2401 gaccctgacc ctcgacgatc tcacggtcgg gcgccaggtc cagcacggcg agctgaccgt
     2461 gaccgtcgcg gcccgcgggc ggaagagcat caccctcgcg acgagccggg acggtgaccg
     2521 gatctactac gtccgcctcc tcggcgccga cggccaggcc ctggcctact tcggcccgaa
     2581 catcaccgag ggtcccggcg gggcgtggcg cttcgacctc tcgccgctca acccgccggt
     2641 caaggcggag atcgtcctgg cggggcaggt ggagcggcag acccttccgg tgagcctcac
     2701 gccccgctag cgcctcgtcg cgtgctatcc tcgcggtcct cacaaggagg tgtggccatg
     2761 aagctcctcg tcgcgctgct cgtaggcatt ctgctgctcg cgcccgcggc cgcgttcgcc
     2821 caggcgccgc tccagctcaa ggccgggagc ggagtcggcc ccggcaaccc ggtgaacgac
     2881 gcgctcttcg atctggccaa gagcctcgat cagaagagcg ggggtcggat caagctcgac
     2941 gtggtcatca gcaacggtct cgccaagggc gaggccgcgc atctcgaggg cgcgcagctc
     3001 ggcaccatcg acatcgtgcc gatcggctcc gcgcccatcg gcggcatgtt cgaggcggcc
     3061 taccaggccc tcgacctgcc cttcttctgg tcctcgcgcg agcaggtgtg gaaggtgatg
     3121 gacggcccga tcggccagga gctgctcaag cggatggagg ccaagggggt gaagggcttt
     3181 tgcttcggcg gcggctgggg cttccgcaac atgatggtca acaagcggcc gatcatggtc
     3241 cccgacgaca tgaaggggct gaccatccgc gtgcaggagt cgcccaccta cgtggcgttc
     3301 atgaaggcgc tcggcgcgaa cccggtgccg atgccctacg tcgaggtcta cctcgccatg
     3361 aagcagggga cgatcgacgg catggagctg ccgtccttca ccatgacctc ggacaagttc
     3421 caggaggtca ccaagtacta ctcgctcacg cgccactcct acccgccaat cgcgtacttc
     3481 atgaacatga agcgctacca gtcgctcgcc ccggatctcc agaaagtggt ggccgactcg
     3541 atgcagcagg cgtgcgccgc gcaccgccgc ttcgaggtgg agaaggagaa gcaggacctc
     3601 gacgtcatga agaaggcggg gacgcaggtg aacgaggtga aggacctgaa ggcgttccag
     3661 gcgctgatga agccggtcta cgcgggggtc gagcagcagg tgggcaagga gttcatggac
     3721 cggctcaccg ccgcggcgca ggccgcgaga taggagccgg gcgatgcccg cctacgtgat
     3781 cgccaacgtg acggtgaccg atctccccgc gatggaggaa taccgcaagc aggtgcccgc
     3841 caccctcgcc aagtacgggg gccgattcct cgtccgcggc ggtgggcacc agacggtcga
     3901 gggcgactgg aagcccaccc ggctggtggt gatcgagttc ccgaccctgg ccgccgccca
     3961 gcgctggtac gactcggagg aataccgcgc gccgcgggcg ctccggatga aggcgggccg
     4021 caccgacatg gtgatggtgg acggcgtcgc gtagcgaggc gcctaccgga agacgatgct
     4081 gctggggctg cccaccgaca cgacctggcc ggtgggagtg agggtgcccg cctgcgggtc
     4141 cacgcggaag gccacgatcg tgtcgg
//
LOCUS       DATMCV010000389         4653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_354658, whole genome shotgun sequence.
ACCESSION   DATMCV010000389 DATMCV010000000
VERSION     DATMCV010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354658"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VNU03_09005"
     CDS             <1..471
                     /locus_tag="VNU03_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWP74642.1"
                     /translation="LVVAAGGIADGRGLVAALALGAAGVWMGTRFIATAEAHGHDNYK
                     QKIAAIDEEGTVVTRAVSGKPNRQIRNAFTREWEGRQAEIEPFPIQYERVGKPASIRA
                     REEGDVEHGSAAAGQSAGLVRDVRPAGRVVASIVEEAEAILQVLGRRLGARGEG"
     gene            468..1271
                     /locus_tag="VNU03_09010"
     CDS             468..1271
                     /locus_tag="VNU03_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP74643.1"
                     /translation="MSGPLAGRVAVVTGGSRGIGRGIALYLAREGADCVITYRRNDAA
                     AAETVAALEATGRRARAERLELAEPDQVGPAFERIGAAFGRVDVLVANAAATAFRPVL
                     EQKPHNVRRTFAITVDAFVAMTQAVVPLMAGRPGRIVLVSGIDSFQAMGGHSVLGAAK
                     AAAEGLVRGLAFELGPLGVTVNAVTPGFIRTESSTYYVTEGLGLDYERATARLREATP
                     VRRHGTPEDVAALVGYLASDAASFVTGQCIVIDGGLTITSPMTRMGEEP"
     gene            1312..3324
                     /gene="shc"
                     /locus_tag="VNU03_09015"
     CDS             1312..3324
                     /gene="shc"
                     /locus_tag="VNU03_09015"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWP74644.1"
                     /translation="MPSSWFEPLQARASYQSEVASESRAALAEAAALACSRAAPALLA
                     RQYPEGYWVGDLLADSTLESDYVLLQLWLHPPRDGEWKPPTWERILRARRAILDRQRR
                     DGGFDIYPDGPADVNATIKAYVALRLSGLEAASEPLRRARDTILGLGGIQEANSYVRI
                     NLSLFGLYPKRHVPTIPVELALVPGGLIYEMSSWTRAIVMPLSIIQAKTDTRPVPAGF
                     DLDELVVPGKTFKLPKRDRLSALFRQLDVALKVWESRGPEMIRKPAIREAEKWILDHT
                     RNSDGLGAIFPSMMYLVMALDALGYPRDHPDLIEGIRQFEGLLTETPDTFYFQPCVSP
                     VWDTGIAMFALGEMGAVAPAPMRRAADWLIAREIRRRGDWSVKRPDLEPSGWAFEFAN
                     EHYPDIDDTAMVLLALQHASASDAAAQERCERRAVAWLVGMQSSDGGWAAFDADNNWA
                     FLNRVPFADHNAMLDPSCPDITGRVLEALCRRGFKLSDPPIERGVGFLLETQERDGSW
                     YGRWGVNYLYGTFLALRGLRVAQDPGASKAMWKAGKWIVSVQNEDGGWGESCASYNEG
                     RFVPAPSTPSQTAWALLGLVAAGQGETEALARGVRHLVETQKADGTWDESLATGTGFP
                     NVFYLRYTLYRNYFPLLALTHARRALDEAERSLRLVQESEPSRPIR"
     gene            complement(3358..3930)
                     /locus_tag="VNU03_09020"
     CDS             complement(3358..3930)
                     /locus_tag="VNU03_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP74645.1"
                     /translation="MDALEQLRAAIPDAARDIRLNLQAVLRGGVLSDAQRWGVAAAAA
                     VAARHRRLRDAILSAAAAVAGPAVVEDAKAAASIMAMNNVYYRFRHMVGKPVYGEKPA
                     GLRMNRLMQPATNRTDFELMALAVSAINGCEMCVRAHEKTVTEGGLTPDHVNDAVRIA
                     ATVYAAAVALEAGESGAGDPAVIEVSVQGA"
     gene            complement(3932..4471)
                     /locus_tag="VNU03_09025"
     CDS             complement(3932..4471)
                     /locus_tag="VNU03_09025"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWP74646.1"
                     /translation="MLTVGDRLPQFNLQATVGLEKGREFAQITDRSYPGRWLVLFSWP
                     MDFTFVCPTEIAEFGKRNGDFQDRDTQVLGMSTDTHFVHLAWRRDHADLKSLPFPMLA
                     DTKRELSTALGILHRGEGVPLRATFIVDPQGVIRWASVNDLSVGRNVDEVLRVLDALQ
                     TGELCPCNWKAGEPTLQVA"
BASE COUNT          645 a         1664 c         1691 g          653 t
ORIGIN      
        1 ctcgtggtgg cggcgggcgg gatcgccgac gggcgcgggc tcgtggccgc gctggcgctg
       61 ggggcggccg gcgtctggat gggcacgcgc ttcatcgcca ccgccgaggc gcacggacac
      121 gacaactaca agcagaagat cgccgccatc gacgaggaag gcacggtggt gacccgcgcg
      181 gtgtccggca agcccaaccg ccagatccgc aatgcgttca cccgcgagtg ggagggccgg
      241 caggccgaga tcgagccgtt cccgatccag tacgagcgcg tgggcaagcc cgcctcgatc
      301 cgggcgcggg aagagggcga cgtggagcac ggcagcgcgg cggccggaca gtcggccggc
      361 ctcgtccgcg acgtgcgccc cgcgggccgg gtggtggcgt cgatcgtgga ggaggccgag
      421 gcgatcctcc aggtgttggg gcgccgcctc ggcgcgcggg gcgaagggtg agcggtccgc
      481 tcgcggggcg ggtcgcggtc gtcaccggcg gctcgcgcgg catcggccgc ggcatcgcgc
      541 tgtacctggc gcgcgagggc gccgactgcg tgatcaccta ccggcgcaac gacgcggcgg
      601 ccgcggagac ggtggccgcg ctcgaggcca ccggccggcg cgcccgcgcc gagcgcctcg
      661 agctggccga gcccgaccag gtggggcccg ccttcgagcg catcggggcc gccttcggcc
      721 gggtggacgt gctggttgcg aacgcggcgg ccaccgcctt ccgcccggtg ctcgagcaga
      781 agccccacaa cgtgcggcgc accttcgcga tcacggtaga cgccttcgtc gcgatgaccc
      841 aggcggtggt gccgctcatg gcgggccggc ccggccgtat cgtgctggtc tcggggatcg
      901 acagcttcca ggccatgggc ggccacagcg tgctgggcgc ggccaaggcg gcggcggagg
      961 gtctcgtccg ggggctcgcg ttcgagctgg gcccgctcgg tgtcaccgtg aacgcggtca
     1021 cgccgggctt catccggacc gagtcgtcca cctactacgt gacggagggg ctcgggctcg
     1081 actacgagcg ggccaccgcc cggctgcggg aggcgacacc ggtgcgccgg cacggaaccc
     1141 ccgaggacgt ggcggccctg gtgggctatc tagcctccga tgcagcctct ttcgtcaccg
     1201 ggcagtgcat cgtgatcgac ggcgggctca ccatcacctc gccgatgacc cgcatgggag
     1261 aggagcccta gagggggtgg ttggggcccc ccttcgtgta gactgggcgt catgccctcg
     1321 tcatggttcg agccgctcca ggcgcgcgca agctaccagt cggaggtagc cagcgagagt
     1381 cgcgccgccc tcgcggaggc cgccgcgctg gcgtgctcgc gcgcggcgcc cgcgctgctg
     1441 gcccgccagt atcccgaggg ctactgggtg ggggacctgc tcgccgattc caccctcgag
     1501 tccgactacg tgctgctgca gctctggctc cacccgcccc gcgacggcga gtggaagccc
     1561 cccacgtggg agcggatcct ccgggcgcgt cgggccatcc tcgaccggca gcggcgcgac
     1621 ggcggcttcg acatctatcc ggacgggccc gccgacgtca acgccaccat caaggcgtac
     1681 gtggccctcc ggctctcggg gctcgaggcc gcgagcgagc cgctgcggcg ggcgcgcgac
     1741 accatcctcg ggctgggcgg catccaggag gccaacagct acgtccgcat caacctgagc
     1801 ctcttcggtc tctatccgaa gcggcacgtg cccaccatcc cggtggagct ggccctggtg
     1861 ccgggcggcc tgatctacga gatgtcgtcg tggacgcgcg cgatcgtgat gccgctctcg
     1921 atcatccagg ccaagaccga cacccggccg gtgccggccg gcttcgacct cgacgagctg
     1981 gtggtccccg gcaagacctt caagctgccg aagcgcgatc gcctctccgc gctcttccgc
     2041 cagctcgacg tggccctcaa ggtgtgggag agccgcggcc ccgagatgat ccggaagccg
     2101 gccatccgcg aggccgagaa gtggatcctc gatcacaccc gcaacagcga cggcctcggc
     2161 gcgatcttcc cctccatgat gtacctcgtc atggcgctcg acgcgctcgg gtacccgcgc
     2221 gaccatcccg atctcatcga gggcatccgg cagttcgagg gcctgctgac cgagaccccc
     2281 gacaccttct acttccagcc ctgcgtttcg ccggtgtggg acaccgggat cgcgatgttc
     2341 gcgctcggcg agatgggcgc ggtggcgccc gccccgatgc gccgggccgc cgactggctc
     2401 atcgcgcggg agatccgtcg ccgcggagac tggtcggtga agcggcccga cctcgagccg
     2461 tccggctggg ccttcgagtt cgccaacgag cactaccccg acatcgacga caccgcgatg
     2521 gtgctgctcg cgctgcagca cgcgagcgcc tccgacgcgg cggcccagga gcgctgcgag
     2581 cgccgggcgg tcgcctggct cgtgggcatg cagtcgagcg acggcggctg ggccgccttc
     2641 gacgccgaca acaactgggc cttcctcaat cgcgtgccct tcgccgacca caacgcgatg
     2701 ctcgacccct cgtgcccgga catcaccggg cgggtgctcg aggcgctctg ccggcggggc
     2761 ttcaagctgt cggacccgcc gatcgagcgg ggcgtgggct tcctcctgga gacccaggag
     2821 cgcgacggga gctggtacgg tcgctggggc gtgaactacc tctacggaac cttcctcgcc
     2881 ctgcgcggcc tgcgggtcgc ccaggatccg ggcgcctcca aggccatgtg gaaggccggc
     2941 aagtggatcg tctcggtgca gaacgaggat ggcgggtggg gcgagagctg cgcgagctac
     3001 aacgagggcc ggttcgtgcc cgcgccgagc actccgtcgc agaccgcgtg ggcgctgctc
     3061 ggcctcgtgg cggccggaca gggcgagacc gaggcgctgg cgcgcggggt gcggcacctg
     3121 gtcgagaccc agaaggccga cggcacgtgg gacgaatcgc tcgccaccgg caccggcttc
     3181 cccaacgtgt tctacctgcg ctacacgctc taccggaact acttcccgct gctggcgctc
     3241 acccacgcgc gccgggccct cgacgaagcg gagcgctcgc tgcgcctcgt gcaggaatcg
     3301 gagccgtccc gcccgatccg ctgagcgcga ccgggcgcga cggccccccg acccgcgcta
     3361 cgcgccctgg accgacacct cgatcacggc cgggtcgccc gctccggact cccccgcctc
     3421 gagcgccacc gccgccgcgt agaccgtcgc ggcgatccgc accgcgtcgt tgacgtgatc
     3481 cggggtgagc ccgccctcgg tcaccgtctt ctcgtgggcg cgcacgcaca tctcgcaccc
     3541 gttgatcgcg ctcaccgcga gcgccatcag ctcgaagtcg gtccggttgg tggccggctg
     3601 catcaagcga ttcatgcgga ggccggccgg cttctcgccg tagaccggct tgcccaccat
     3661 gtgacggaag cggtagtaga cgttgttcat cgccatgatc gaggcggcgg ccttggcgtc
     3721 ctccaccacc gcggggcccg cgacggcggc cgccgcggac aggatcgcgt cgcgcagacg
     3781 ccggtggcgg gcggcgaccg cggcggcggc cgccaccccc cagcgctgcg cgtccgagag
     3841 caccccgccc cgcagcaccg cctgcaggtt caggcggatg tctcgagccg cgtcgggaat
     3901 ggcggcgcgc agctgctcga gcgcgtccac gtcaggccac ctggagcgtg ggctcgcccg
     3961 ccttccagtt gcacgggcag agctcgccgg tctgcagcgc gtccagtacc cgcagcacct
     4021 cgtcgacgtt gcggcccacc gagaggtcgt tgacgctggc ccagcggatc acgccctggg
     4081 gatccacgat gaaggtggcc cggagcggca cgccctcgcc gcggtgcagg atgccgagcg
     4141 cggtcgacag ctcgcgcttg gtgtcggcca gcatcgggaa cggcaggctc ttgaggtcgg
     4201 cgtgatcgcg ccgccacgcg aggtggacga agtgggtgtc ggtgctcatg ccgagcacct
     4261 gggtatcgcg gtcctggaag tcgccgttcc gcttgccgaa ctcggcgatc tcggtcgggc
     4321 agacgaacgt gaagtccatc ggccaggaga agaggaccag ccagcgcccg ggatagctcc
     4381 ggtcggtgat ctgcgcgaat tcacggccct tctcgaggcc gacggtggcc tgcaggttga
     4441 actggggtag acggtcgcca acggtcagca tatcgggctc ctttgtggtc gcattagggt
     4501 gacttcggct tccctacttc ggatgattag ggtaccctaa tcgatgcagg cgggcgaggg
     4561 aaaggcggga ggcggatcgg gctacggcag cgcggcggct ccagcccggg ccagggccgg
     4621 cagctcggga tcgcgcgcgc ccatgatgag cac
//
LOCUS       DATMCV010000390         5586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_886778, whole genome shotgun sequence.
ACCESSION   DATMCV010000390 DATMCV010000000
VERSION     DATMCV010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886778"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VNU03_09030"
     CDS             <1..446
                     /locus_tag="VNU03_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWP74647.1"
                     /translation="VEPVVHEVLESFRHPLHQQGFALEVSIAPDLPDVPLDGEALKQA
                     LANLLDNAMKYSADRRKIAVTARREGDGVAVAVADEGIGIPLSERERIFEKFYRVGRS
                     ETQGRRGSGVGLALVKHIVEAHGGRVTVDGGPGVGSRFTLHLPGA"
     gene            454..1149
                     /locus_tag="VNU03_09035"
     CDS             454..1149
                     /locus_tag="VNU03_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWP74648.1"
                     /translation="MPRILIVDDEPEMVRGLEDNLRFEGYQTVAAPDGRRGLALALSE
                     APDLILLDVMMPGMSGWDLCRELRRRGLDVPVIMLTARGEEVDRVLGLELGADDYVTK
                     PFSLRELMARIRAVLRRPGPRQKFEEFAFGDVRVHLRARQAFKSGREVRLTRKEFDLL
                     RYLIEHPGEVITRDRLLDEVWGYEQFPTTRTVDTHILRLRQKFEDDPERPAHILTAHG
                     QGYRFVAERGASS"
     gene            1146..1787
                     /locus_tag="VNU03_09040"
     CDS             1146..1787
                     /locus_tag="VNU03_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HWP74649.1"
                     /translation="MSEIARDLARRTPPGQVLTTKWPVLTYGRTPRFDPRKWSFRAFG
                     LVEEETSFSWEELQALPRVEVACDIHCVTRWSRLDNRFEGVSVREIVRRVRPKPEAKY
                     VMVHADPDYTTNLALADLVDDDVVLALRHDGQDLTPDHGGPLRLVVPKLYFWKSAKWV
                     RGFEFLDVNPPGFWEVNGYHMHADPWAEERYSDQETHAMQTMRAEAARRLRAR"
     gene            complement(1896..2723)
                     /locus_tag="VNU03_09045"
     CDS             complement(1896..2723)
                     /locus_tag="VNU03_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP74650.1"
                     /translation="MSFLERTAKVNGITLHYLDWGPADAPPVVLLHGITGHGRVWDHL
                     AARLVPQRRVLALDQRGHGDSDPAPDDDYRVGTMADDVAAFAGSLRLPRFALVGHSMG
                     GRIAIKYAGDHAARLERLVIIDIGPDINLAGLQRVRDMMAKSPERIESEEWAVEYIRR
                     ANPLQDVDMLRERVRHGLKRLPDGELTWKYAKGLRDMMREGRRDAVDLWEPLPRIPCP
                     TLIVRGAESDILSAEVAKKMAERLPDGRVVEIGGAGHTVPADRPEEFVRTIREFLGA"
     gene            2867..4411
                     /locus_tag="VNU03_09050"
     CDS             2867..4411
                     /locus_tag="VNU03_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HWP74651.1"
                     /translation="MATHAITDVAIVLKPEDDVAIAKKEIPAGTILEDAGGRIEVRQD
                     IKPGHKVARRARRTGETVRRYGQPIGFATADIAVGDHVHTQNVAVGDLKQQYEVGTDV
                     QPVAFYPPDEMRYFDGYLRDDGRVGTRNYVAVISGVNCSASVSQFVKDKFRDVTRDYP
                     NIDGVLAITHKSGCGTKLFGEDHLALQRVLAGYAKHPNIAAYILIGLGCEVNQAAVMV
                     DRQRMAAPGHPERKPFVVNIQEAGGIRKTVETAVREVAKLLPLANAAKRTKQPVSELT
                     LATNCGGSDGASGITANPALGWAVDELVRYGGTGVLAETPEIYGAEHLLIRRAINEGV
                     AKKLIDRYKWWEWYCRGIEAMDNNPAPGNKAGGITTVYEKSLGGVSKGGTTPMMDVFQ
                     YGEPITTRGFVFMDTPGHDPVSITGLVAGGCNMICFTTGRGSVFGCKPVPSIKLATNS
                     LMYRHMSEDMDINCGVILEGTPLPTVGRQIFEEIVEVASGKRSKSELSGVGEEEFAPW
                     IIGPVM"
     gene            complement(4589..5143)
                     /locus_tag="VNU03_09055"
     CDS             complement(4589..5143)
                     /locus_tag="VNU03_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74652.1"
                     /translation="MSPGGHLVTTAAACAAVYAGTGSVELVAGLAAGGFLIDVDHVVD
                     YVLFERQRDLRPASFLRYYLGGRVERVVLVLHSYELLVLLAALAWFTRIDWLWGWVFG
                     MLLHLPLDIIFNAKFASGGFVHFYSFIVRARAGFRADRFTDRRVVPVPPDEFWRPFFA
                     GGRLADEVVEPPAPPEPRSVAVRG"
     gene            complement(5154..5279)
                     /locus_tag="VNU03_09060"
     CDS             complement(5154..5279)
                     /locus_tag="VNU03_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74653.1"
                     /translation="MLKRLGLRILDLIDTIPLGWLEPYRRPVPVPVRRLRRPYPR"
BASE COUNT          948 a         1851 c         2038 g          749 t
ORIGIN      
        1 cggtggagcc ggtcgtccac gaggtgctcg agagcttccg ccaccccctc caccagcagg
       61 gcttcgcgct ggaggtgtcg atcgccccgg acctgcccga cgtgccgctg gacggggagg
      121 ccttgaagca ggccctcgcc aacctgctcg acaacgccat gaagtactcg gcggaccggc
      181 ggaagatcgc ggtcaccgcc cgtcgggagg gcgacggggt tgcggtggcg gtggcggacg
      241 aggggatcgg gatcccgctc tccgagcgcg agcgaatctt cgagaagttc taccgcgtcg
      301 gtcggagcga gacgcagggt cgccggggaa gcggggtggg gctggcgctg gtcaagcaca
      361 tcgtcgaggc ccacggcggc cgggtgacgg tggacggcgg gcccggcgtg ggcagccggt
      421 tcacgttgca tctcccggga gcgtgaggac gcggtgccgc gcatcctgat cgtcgacgac
      481 gagcccgaga tggtgcgcgg gctggaggac aacctgcgct tcgagggcta tcagaccgtc
      541 gcggcgccgg acgggcgccg agggctggcc ctggccctgt cggaggcgcc ggacctgatc
      601 ctgctcgacg tcatgatgcc gggcatgagc ggctgggacc tctgccgcga gctgcggcgg
      661 cgcgggctcg acgtgccggt catcatgctg accgcgcggg gcgaggaggt ggaccgggtg
      721 ctcgggctcg agctcggcgc ggacgactac gtcaccaagc cgttcagcct gcgggagctg
      781 atggcgcgca tccgggccgt gctccgccgg ccgggcccgc ggcagaagtt cgaggagttc
      841 gcgttcggtg acgtgcgggt gcacctccgg gcgcgccagg ccttcaagag cgggcgcgag
      901 gtgcggctca cccgcaagga gttcgacctg ctgcgctacc tgatcgagca cccgggcgag
      961 gtcatcactc gcgaccggct cctcgacgag gtgtggggct acgagcagtt cccgaccacc
     1021 cgcaccgtcg acacgcatat cctgaggctg cggcagaaat tcgaggacga tccggagcgg
     1081 cccgcgcaca tcctcaccgc gcacgggcag ggctaccgct tcgtcgccga gagaggagcg
     1141 tcatcatgag cgagatcgcc cgggacctgg cccggcgcac gccgcccggc caggtgctga
     1201 ccacgaaatg gccggtgctc acctacggcc ggaccccgcg cttcgacccc cggaagtggt
     1261 cgttccgcgc cttcggcctc gtcgaggagg agacgagctt cagctgggag gagctccagg
     1321 ccctgccccg cgtcgaggtc gcgtgcgaca tccactgcgt gacgcgctgg tcgcgactcg
     1381 acaaccgctt cgagggcgtg tccgtccggg agatcgtgcg acgcgtccgg ccgaagcccg
     1441 aggcgaagta cgtcatggtc cacgccgacc ccgactacac caccaacctg gccctggcgg
     1501 acctggtcga cgacgacgtg gtgctggccc tccggcacga cggccaggac ctcacgccgg
     1561 accacggcgg accgctgcgc ctggtggtgc cgaagctcta cttctggaag agcgcgaagt
     1621 gggtgcgcgg cttcgagttc ctggacgtga acccgcccgg cttctgggag gtgaacggct
     1681 accacatgca cgccgatccc tgggccgagg agcgctactc cgaccaggag acgcacgcga
     1741 tgcagacgat gcgggccgag gccgctcgcc gcctccgcgc ccgctaaccc tcaatacccc
     1801 tctcccctct ggggagaggg cagggtgagg ggcccgaggc tgaggcgtcc gaggctgagg
     1861 ggtccgaggg tgaggggccc gaggccgagg agaccctacg ccccgaggaa ctcgcggatc
     1921 gtccgcacga actcctccgg ccgatccgcg ggcacggtgt ggccggcgcc cccgatctcg
     1981 accacgcgcc catcgggcag ccgctccgcc atcttcttgg ccacctcggc ggagaggatg
     2041 tcgctctccg cgcctcggac gatcagggtg gggcagggaa tgcgcggcag cggctcccag
     2101 agatccaccg cgtcgcgccg gccctcgcgc atcatgtcgc gcagcccctt ggcgtacttc
     2161 caggtcagct cgccgtccgg cagccgcttg agcccgtggc ggacccgctc gcggagcatg
     2221 tcgacatcct ggagcggatt ggcgcgccgg atatattcca ccgcccactc ctcgctctcg
     2281 atgcgctcgg gggacttggc catcatgtcg cgcacccgct ggagcccggc caggttgatg
     2341 tcggggccga tgtcgatgat cacgaggcgc tcgagccggg ccgcgtgatc tcccgcgtac
     2401 ttgatcgcga tgcgcccacc catcgagtgg ccgaccaggg cgaagcgcgg caggcgcagg
     2461 ctgcccgcga aggcggccac gtcgtcggcc atggtgccca cccggtagtc gtcgtcgggc
     2521 gcgggatcgc tgtcgccgtg cccgcgctgg tcgagcgcga gcacccggcg ctgcggcacg
     2581 agccgtgcgg ccaggtggtc ccacacccgc ccgtggccgg tgatgccgtg cagcagcacc
     2641 accggcgggg cgtcggccgg gccccagtcg agatagtgca gcgtgatgcc gttgaccttg
     2701 gcggtgcgct cgaggaagga catgccgggc agtctagtct gcagatggcc tccggtcaaa
     2761 ctcgcgcggc ttgcctatcg tcgacgcggg acggtagagt gggctacccg gctgcccctc
     2821 accccggccc tctcccccga ggggagaggg ggaagagaga cccgacatgg cgacgcacgc
     2881 gatcacggat gtggcgatcg ttctgaagcc cgaagacgac gtggcgatcg ccaagaagga
     2941 gatcccggcc ggcaccatcc tggaggacgc gggcggccgc atcgaggtca ggcaggacat
     3001 caagccgggc cacaaggtcg cgcggcgggc gcggcgcacc ggggagacgg tccgccgcta
     3061 tgggcagccc atcgggttcg ccaccgccga catcgcggtc ggcgaccacg tccacaccca
     3121 gaacgtggcg gtgggggacc tcaagcagca atatgaagtg ggcaccgatg ttcagccggt
     3181 cgcgttctat ccgccggacg agatgcgtta cttcgacggt tatcttcgag acgacggtcg
     3241 agtcggcacc cgcaattatg tagcggtgat ctcgggggtg aactgctcgg ccagcgtgag
     3301 ccagttcgtg aaggacaagt tccgcgacgt gacgcgcgac tatcccaaca tcgacggggt
     3361 gctcgcgatc acccacaagt cgggctgcgg caccaagctc ttcggcgagg accacctggc
     3421 gctccagcgg gtgctggccg gctacgccaa gcacccgaac atcgcggcct acatcctgat
     3481 cgggctcggc tgcgaggtga accaggccgc ggtcatggtc gaccggcagc gcatggccgc
     3541 gccgggccat ccggagcgga agcccttcgt ggtcaacatc caggaggccg gtgggatccg
     3601 gaagacggtg gagaccgcgg ttcgcgaggt ggccaagctg ctgcccctgg ccaacgcggc
     3661 gaagcgcacg aagcagccgg tgtcggagct cacgctcgcc accaactgcg gcggctccga
     3721 cggagcctcc ggcatcaccg cgaaccccgc gctcggctgg gcggtcgacg agctagtgcg
     3781 ctacggcggc accggggtgc tcgcggagac cccggagatc tacggggccg agcacctgct
     3841 gatccgccgc gcgatcaacg agggcgtggc caagaagctg atcgaccggt acaagtggtg
     3901 ggagtggtac tgccgcggca tcgaggcgat ggacaacaac cccgcccccg gcaacaaggc
     3961 gggcggcatc accaccgtct acgagaagtc gctcggcggc gtgagcaagg gcggcaccac
     4021 cccgatgatg gacgtgttcc agtatggcga gccgatcacc acgcgcggct tcgtgttcat
     4081 ggacaccccg gggcacgacc cggtgtcgat caccgggctc gtcgcgggcg gctgcaacat
     4141 gatctgcttc accaccggac gcggctccgt gttcggctgc aagccggtgc cctcgatcaa
     4201 gctcgccacc aacagcctca tgtaccggca catgtcggag gacatggaca tcaactgcgg
     4261 ggtgatcctg gagggcacgc cgctgcccac ggtcggccgc cagatcttcg aggagatcgt
     4321 ggaggtcgcc tcgggcaagc gcagcaagag cgagctctcc ggcgtcggcg aggaggagtt
     4381 cgcgccgtgg atcatcgggc cggttatgta gtgtgaggtg aagccgaagg caagccgaac
     4441 gcattccaaa aatgcagtaa ggaagtaaag catcgagagc tgtaccgcga gggtaacagc
     4501 gcggccagga gaccgcagcg gcgggaggcg caggccgaat gccagggaac ccgggaatgt
     4561 atcccggcaa ccggcgccgc agggaagatc acccgcggac cgcgacggag cggggctcgg
     4621 gcggggcggg cggctcgacg acctcgtcgg ccagccgtcc tcccgcgaag aacgggcgcc
     4681 agaactcgtc cgggggcacc ggcacgacgc ggcgatcggt gaagcggtcg gcccggaagc
     4741 cggcgcgggc gcgcacgatg aagctgtaga agtggacgaa cccgcccgag gcgaacttgg
     4801 cgttgaagat gatgtcgagc ggcagatgca gtagcatgcc gaagacccag ccccacagcc
     4861 agtcgatccg ggtgaaccac gcgagcgcgg ccagcaggac cagcagctcg taggagtgga
     4921 gcacgaggac gacccgctcg acgcgcccgc cgaggtaata ccggaggaac gaggcggggc
     4981 ggaggtcgcg ctggcgctcg aagagcacgt agtcgaccac gtgatcgacg tcgatcagga
     5041 agccgcccgc ggccaggccg gccacgagct cgacgctgcc ggtgcccgcg tagaccgcgg
     5101 cgcaggccgc agcggtggtg acgagatgtc ctccggggct catggcgctt cggctaccgc
     5161 gggtaggggc ggcggagacg gcggaccgga accgggaccg gccggcggta tggctcgagc
     5221 cacccgagcg ggatcgtgtc gatcaggtcg aggatgcgaa gaccgagtcg cttcaacatg
     5281 gagcccagag ggagcaactc cggtgccagt ggcgcccggg ctgcaagtac ctgaattttc
     5341 gagagaccat gggctgggtg gggacttcgt gggccaaaac aggaagatcc taggaccaaa
     5401 atggggagat cgatgccagg agagtgggca tgacgacgag aagggcaacc gggatcggct
     5461 tcgctgagat cgtctcctcc gagtcgacgg cctgggagat ccagaccacc ggtccgggcc
     5521 cgcagggcag cctgcccctc accgccgaga tgctgctcga gcggccgagc ggcgacatct
     5581 tcggga
//
LOCUS       DATMCV010000391         2485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_220411, whole genome shotgun sequence.
ACCESSION   DATMCV010000391 DATMCV010000000
VERSION     DATMCV010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220411"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VNU03_09065"
     CDS             <1..184
                     /locus_tag="VNU03_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWP74654.1"
                     /translation="EYWIVNLIDRVVEVYRDPGPDALAVHGWRYRSVARLGLAGTVEP
                     VALPSARVRVADLLLR"
     gene            complement(198..653)
                     /locus_tag="VNU03_09070"
     CDS             complement(198..653)
                     /locus_tag="VNU03_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74655.1"
                     /translation="MAMREIAFALEFKGRGGPVAGSTTERRARTTAPSQALRTVLAPD
                     GISGSVEALPGESAVLESRVERFPDGTFAEDGTITYGRAGSISFDTVGRGHVGPAAGG
                     IGSHGVVMWRITGGDGRFAGAQGLITSNFTVTPDGLVTDDHFTRLYLPE"
     gene            complement(653..1744)
                     /locus_tag="VNU03_09075"
     CDS             complement(653..1744)
                     /locus_tag="VNU03_09075"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP74656.1"
                     /translation="MRAAVLFEQGKPQRVEEVALEAPRVGEVSVRMVASGVCHSCLHA
                     ADGSWKGVPVPIVLGDEGAGVVEALGPGVTELAVGDHVILSWAPTCGRCHYCVIGRPN
                     LCERRQPGKGVLPDGTTRMSLGGSPVYHYGHVATYASRTVVHESCAIRIDPAMPLDRA
                     ALVGCSVMTGVGAVLNTAAVPAGASMAVFGVGGVGLNVVQGGAMVAAHPIIAVDLLPT
                     KLEHARAVGATHLIDASRQDPAAAIRAITGRGADFTFVAVGATRPITQAIDALAPGGT
                     CVLIGVPETGATVPLDARPMVTGERVIRGSSYGSARTREDLPRLVALYQAGKLRIDEL
                     ITHRYALEDADEAFRALAAGELGRGIIVF"
     gene            complement(1762..>2485)
                     /locus_tag="VNU03_09080"
     CDS             complement(1762..>2485)
                     /locus_tag="VNU03_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74657.1"
                     /translation="DLGTSWRTGKPVVALILERLPVTLELTLFSVAFALVLGIAAGIV
                     SAVRRDRAIDNVTRVGTLLGLSVPVFWQGTMLILFFSLYLRWMPPVVWVDFSADWRRN
                     LTIMVLPALCLGTASAANVARTTRSCMLDVLGSEYVRTAAAKGLTGRVVVLKHALRNA
                     LIPVVTVAGLQIGILLGGTVVVEEVFTLPGIGRLVLWSIYQRDYPLTQSTILFIAVLF
                     MAINLAVDLLYGYLDPRIRYGR"
BASE COUNT          365 a          867 c          942 g          311 t
ORIGIN      
        1 ggagtactgg atcgtcaacc tgatcgaccg cgtcgtggag gtgtatcgcg acccgggacc
       61 ggacgcattg gccgtgcacg gctggcggta tcgctcggtg gcgcgtctcg gactcgccgg
      121 gacggtggag ccggtggcgc tgccgtcggc gcgggtgcgc gtcgccgacc tgttgcttcg
      181 gtgagccgcc gcccgcgcta ctccggcagg tagaggcggg tgaagtggtc gtcggtgacg
      241 agcccgtccg gcgtcaccgt gaagttggag gtgatcagcc cctgcgcccc cgcgaagcgg
      301 ccgtcgccgc cggtgatgcg ccacatcacc acgccgtggc tcccgatgcc ccccgcggcg
      361 gggccgacgt gaccccggcc gacggtgtcg aacgagatcg acccggcgcg gccgtaggtg
      421 atggtgccgt cctccgcgaa ggtgccgtcg gggaagcgct ccaccctcga ctcgaggacg
      481 gcggactcgc ccggtagcgc ctccaccgat ccgctgatcc catccggtgc gagcaccgtg
      541 cgcagcgcct ggctcggcgc ggtggtgcgc gcgcggcgct cggtggtcga gccggccacc
      601 gggcccccgc ggcccttgaa ctcgagcgcg aacgcgatct cgcgcatggc catcagaaca
      661 cgatgatgcc gcggccgagc tcgccggcgg cgagcgcccg gaaggcctcg tcggcatcct
      721 cgagcgcgta gcggtgggtg atcagctcgt cgatgcggag cttgcccgcc tggtagagcg
      781 cgaccagccg gggcaggtcc tcgcgggtgc gcgcgctgcc gtagctcgag ccgcggatga
      841 cgcgctcgcc ggtgaccatc gggcgcgcgt ccagcggcac ggtcgcgccg gtctcgggca
      901 ccccgatcag cacgcaggtg ccgccgggcg cgagggcgtc gatggcctgg gtgatgggac
      961 gggtggcgcc caccgccacg aaggtgaagt cggcgccgcg gccggtgatc gcgcggatgg
     1021 cggcggcggg atcctggcgg gacgcgtcga tcaggtgggt cgccccgacc gcgcgggcgt
     1081 gctcgagctt ggtggggagc aggtcgaccg cgatgatcgg gtgcgccgcc accatcgcgc
     1141 cgccctggac cacgttgagc ccgacgccgc ccaccccgaa gacggccatg ctcgcaccgg
     1201 ccggcaccgc cgcggtgttg agcaccgcac ccaccccggt catgaccgag cagccgacga
     1261 gcgcggcgcg gtcgagcggc atcgcggggt cgatgcggat cgcgcacgac tcgtgcacca
     1321 cggtgcgcga ggcgtaggtg gccacgtggc cgtagtggta gaccggggag ccgccgagac
     1381 tcatccgggt ggtcccgtcg gggagcacgc ccttgccggg ctggcggcgc tcgcagaggt
     1441 tcggccggcc gatgacgcag tagtggcagc gaccgcaggt gggcgcccac gagaggatca
     1501 cgtgatcgcc gaccgcgagc tcggtcacgc cgggcccgag cgcctccacc acgcccgcgc
     1561 cctcgtcgcc cagcacgatc ggcacgggca cgcccttcca gctcccgtct gccgcgtgca
     1621 ggcagctgtg gcagaccccg ctggccacca tgcgaacgga gacctcgccg acccgcggcg
     1681 cctcgagcgc cacctcttcc acccgctggg gcttgccctg ctcgaagagc accgctgcgc
     1741 gcatgaacgc ctccgtggga atcagcggcc gtaccggatc cgcggatcga ggtagccgta
     1801 gagcaggtcg acggccaggt tgatggccat gaacagcacc gcgatgaaca ggatggtgga
     1861 ctgggtgagc gggtagtcgc gctggtagat cgaccagagc acgagccggc cgatgccggg
     1921 cagggtgaag acctcctcga ccaccaccgt gccgccgagc aggatgccga tctgcagccc
     1981 ggccaccgtg accaccggga tcagcgcgtt gcggagggcg tgcttgagca ccaccacgcg
     2041 gccggtgagg cccttggccg ccgcggtgcg gacgtactcg gagccgagca catcgagcat
     2101 gcacgagcgg gtggtgcggg ccacgttggc cgcgctcgcg gtgccgaggc agagggcggg
     2161 cagcaccatg atcgtgaggt tgcggcgcca gtcggcgctg aagtcgaccc acaccaccgg
     2221 cggcatccag cgcaggtaga gcgagaagaa gaggatcagc atggtgccct gccagaacac
     2281 cggcaccgag aggccgagga gcgtgccgac ccgggtgacg ttgtcgatgg cgcggtcgcg
     2341 ccgcaccgcc gagacgatgc cggccgcgat gccgagcacc agggcgaagg ccaccgagaa
     2401 gagggtcagc tcgagggtga ccggcaggcg ctccaggatc agggccacca ccggcttgcc
     2461 ggtccgccac gaggtgccga ggtcg
//
LOCUS       DATMCV010000392         5576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_956493, whole genome shotgun sequence.
ACCESSION   DATMCV010000392 DATMCV010000000
VERSION     DATMCV010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956493"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VNU03_09085"
     CDS             <1..1172
                     /locus_tag="VNU03_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP74658.1"
                     /translation="GMLAIIRDMTAQREIEAQMHQSERLTSLGQMAGGIAHDFNNLLQ
                     AILGYAQLMARSPGNVDVVKRGIDVIEKAATSGAETVRRMQKFARIRQQDEPFVPMDV
                     NQVVRDSLAITRPRWEEKKVKGGVPLQLELELGPVPVVMGRPAELNEVITNLVLNAID
                     AMPRGGTLRIRTRLADYRHAAITVADTGMGMTDEVRKRVFDPYFTTKGEEGTGLGLSV
                     SHSIVERHGGDLRVDSRPGEGTTFTITLPIGMKPTSESTTGSHPGGERTGRILLVDND
                     PQVLSILGEMLKDAGHHVLPVPSGPEALRVFIPSGFDLIITNVGMPEMSGWDVAERLR
                     ASDPQVPVIFITGWGLQDEDQARSRRLGISALLFKPVSPQDLHRTVQVALAARTR"
     gene            complement(1257..2402)
                     /locus_tag="VNU03_09090"
     CDS             complement(1257..2402)
                     /locus_tag="VNU03_09090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWP74659.1"
                     /translation="MHNAVIVGAVRTAVGRKNGKLAGVRPDDLLADTLKALVERVKID
                     PSEVEDVVMGCVDQVGEQGFNIARNAALIAGFPLDVCGTTLDRMCGSGQQAANFAAMG
                     VMSGQYECVIAGGVENMSRVPMGSNGAGPGDGPLSPRLQALYNIVPQGLSAEMIAEQW
                     GIKREELDEFSAGSHERAGRAIAEGRFKREIAPITLPDGSVFEVDEGVRVPVNREKMA
                     ALAPSFKPDGVVTAANSSQISDGAAALLIMSEARAKALGLKARARIVATALAGVDPTI
                     MLTGPIPATQRVLKKAGLKLDQMDLFEINEAFASVVLAWERELHPDMSRVNVNGGAIA
                     LGHPLGCSGGKLMTTLLHELERTKKRYGLQTMCIGFGQGIATIIERL"
     gene            complement(2416..3321)
                     /locus_tag="VNU03_09095"
     CDS             complement(2416..3321)
                     /locus_tag="VNU03_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP74660.1"
                     /translation="MKCRKCGGAASLELRRHNAAFCSPHFIEFFRKQVAEAVHRHHMF
                     TTDETIMVAVSGGKDSLALWDVLIEDGYRTAGLYLDLGIFDYSKESRAKCEAFAEARG
                     LTLHVERVAEAVGAPIPVVQQATRRPTCSACGLSKRYLMNRAALEHGYPVVATGHNLD
                     DEAATLFGSVMHWQTDALPRQSPALASTHPKLVRRVKPLYRLSELETAAYAFLRGIDY
                     IVEECPFAKGATSIAHKQVLDRLEEASPGSKHNFLFGFLDRARPAFERVENVELHECT
                     SCGQVTTGAVCAFCKLADLVKRRTS"
     gene            complement(3318..3527)
                     /locus_tag="VNU03_09100"
     CDS             complement(3318..3527)
                     /locus_tag="VNU03_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWP74661.1"
                     /translation="MKVILRNPRREVDVDGKRRVKDLLSELGILPSTVLVIRDNELLT
                     SDAVVGGDDVLEVRPVMSGGSGAGA"
     gene            complement(3524..4063)
                     /locus_tag="VNU03_09105"
     CDS             complement(3524..4063)
                     /locus_tag="VNU03_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP74662.1"
                     /translation="MAWPAPVETLWNDMESVRAQLLKEAEGLSQAQSNWRPAPDEWSV
                     GEILHHLTLAEVGTGKLTSKLLRDAPQPLPPFPPDLQAFSPLPPPPPGPARAPEVVFP
                     ERGHALERLIADLKTIRERSRQSVERLAAVDPRGLRWRHVALGEIDLAQWWLLHVRHE
                     TDHLQQLRDVKAATGFPAS"
     gene            4101..4718
                     /locus_tag="VNU03_09110"
     CDS             4101..4718
                     /locus_tag="VNU03_09110"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWP74663.1"
                     /translation="MTQPTPADPGESPPGRPASDPPASNPLARTPWRATSTRPIYRNR
                     WIDVREDIVELPDGHTTVYGVVECSECVGVLPFLDPDTVLLVGQYRYVAKAFFWEMPT
                     GGQDAGETRLQAAQRELAEEAGYEAVRHVELGDFHTSKSILREVAHLYLAEDLRPAAR
                     RADHTEFIERRAFPFAEVLAMVTRGEIKDAMTIIAVLHAARRRGM"
     gene            complement(4728..5489)
                     /locus_tag="VNU03_09115"
     CDS             complement(4728..5489)
                     /locus_tag="VNU03_09115"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWP74664.1"
                     /translation="MNRDEIDELAAAYALGGLEGEDRVRFEALLEAGDPDALRALRDC
                     EDTLVGLAAAAPEVPPLGVKAALMERIAATPAPVRPLPRRPRPLWPVVLSGAMAAGLA
                     AVVVGWSVSATYEKRLDALAQSAEQLRAELRSQQTVIAILRDPATQVVALAGLPPAPA
                     ARARMMWHAQAGGVFVATGLPAPPDGKAYQLWAIAGGNAPVSAGVFTVDAAGTGSLAV
                     RPLPGVAAVNAFAVTLEPAGGLPAPSGEMYLLGKS"
BASE COUNT          798 a         2064 c         1941 g          773 t
ORIGIN      
        1 acggcatgct cgccatcatc cgcgacatga ccgcgcagcg ggagatcgag gcgcagatgc
       61 accagtccga gcgcctcacc tccctcggcc agatggcagg cgggatcgcg cacgacttca
      121 acaacctgct gcaggccatc ctcggctacg cccagctcat ggcgcgcagc cccggcaacg
      181 tcgacgtggt caaacgcggc atcgacgtga tcgagaaggc ggcgacgagc ggagccgaga
      241 ccgtccggcg catgcagaag ttcgcgcgga tccgccagca ggacgagccg ttcgtgccga
      301 tggacgtcaa ccaggtggtc cgcgactcgc tcgccatcac gcggccccgc tgggaggaga
      361 agaaggtcaa gggcggcgtg ccgctccagc tcgagctgga gctcggcccg gtgccggtgg
      421 tgatgggccg gcccgcggag ctgaacgagg tcatcaccaa cctcgtgctc aacgccatcg
      481 acgcgatgcc tcgcggcggc actctccgca tccgcactcg cctcgccgac tatcgccacg
      541 cggccatcac ggtggccgac accggcatgg gcatgaccga cgaggtccgc aagcgggtct
      601 tcgaccccta cttcaccacc aagggcgagg agggcaccgg gctcggtctc tcggtctcgc
      661 actcgatcgt ggagcgtcac ggcggcgatc tgcgggtgga cagccgaccg ggcgagggca
      721 ccacgttcac gatcaccctg cccatcggca tgaagccgac cagcgagagc accaccggga
      781 gccatcccgg cggcgagcgc accggccgaa tcctgctggt ggacaacgat ccccaggtgc
      841 tctccatcct gggggagatg ctcaaggacg cgggacacca cgtgctgccc gtgccgagcg
      901 ggcccgaggc gctccgcgtc ttcatcccga gcggcttcga cctgatcatc accaacgtgg
      961 gcatgcccga gatgagcggc tgggacgtgg ccgagcgcct gcgcgcgagc gacccgcagg
     1021 tgccggtgat cttcatcacc ggctggggtc ttcaggacga ggaccaggcg cgctcccgcc
     1081 ggctcggcat ctccgcgctc ctgttcaagc cggtgtcccc ccaggacctg caccgcaccg
     1141 tgcaggtcgc gctggccgcg cgcacccgct agcgcggcaa cccgcggaga cacgacggcc
     1201 gcccgggagg cttccgggcg gccgcgcctt taccgcttcc cgccggtcgg agcgggctag
     1261 agccgctcga tgatggtcgc gatcccctgc ccgaacccga tgcacatcgt ctgcagcccg
     1321 tagcgcttct tggtgcgctc cagctcgtgc agcagggtgg tcatgagctt gccccccgag
     1381 cacccgagcg ggtgccccag cgcgatcgcg ccgccgttga cgttcacccg gctcatgtcc
     1441 gggtgcagct cgcgctccca cgccagcacc accgaggcga acgcctcgtt gatctcgaag
     1501 aggtccatct ggtcgagctt caggccggcc ttcttgagga cgcgctgggt ggccgggatc
     1561 ggaccggtca gcatgatggt gggatcgacg ccggccagcg cggtggccac gatccgcgcc
     1621 cgggccttga gcccgagggc cttggcccgc gcctccgaca tgatgaggag cgcggcggcg
     1681 ccgtcggaga tctgcgagga gttcgccgcg gtcaccaccc cgtccggctt gaaggacggc
     1741 gccagggccg ccatcttctc gcggttcacc ggcacccgca cgccctcgtc gacctcgaag
     1801 acgctgccgt ccggcagggt gatcggcgcg atctcccgct tgaaccgccc ctccgcgatg
     1861 gcccgccccg cccgctcgtg gctgccggcg gagaactcgt cgagctcctc gcgcttgatg
     1921 ccccactgct cggcgatcat ctcggcggag agcccctggg ggacgatgtt gtagagggcc
     1981 tgcagccgcg gggagagcgg cccgtcgccg ggcccggccc cgttcgagcc catgggcacg
     2041 cgactcatgt tctcgacgcc gcccgcgatc acgcactcgt actggccgga catcactccc
     2101 atcgcggcga agttggccgc ctgctggccc gagccgcaca tgcggtcgag ggtggtgccg
     2161 cagacgtcca gcgggaaccc cgcgatgagc gccgcgttgc gcgcgatgtt gaacccctgc
     2221 tcccccacct ggtccacgca gcccatcacc acgtcctcga cctcgctcgg gtcgatcttg
     2281 acgcgctcga cgagcgcctt gagcgtatcg gccagcaggt cgtcggggcg caccccggcc
     2341 agcttgccgt tcttccggcc gacggcggta cggaccgcgc ccacgatcac cgcgttgtgc
     2401 atggctatct cctcgtcatg atgtgcgccg cttgaccaga tcggcgagct tgcagaaggc
     2461 gcagaccgcc ccggtggtca cctggccgca cgaggtgcac tcgtgcagct ccacgttctc
     2521 cacccgctcg aacgcaggcc gggcgcggtc gaggaagccg aaaaggaagt tgtgcttgga
     2581 cccgggcgag gcctcctcca ggcggtccag cacctgcttg tgggcgatcg acgtggcccc
     2641 cttggcgaac gggcactcct ccacgatata gtcgatgccg cgcaggaacg cgtaggccgc
     2701 ggtctcgagc tcagagagcc ggtagagggg cttcacgcga cggaccagct tggggtgcgt
     2761 ggaggccagc gcgggcgact ggcgcggcag ggcgtcggtc tgccagtgca tcaccgagcc
     2821 gaacagggtg gccgcctcgt cgtccaggtt gtggccggtc gccaccaccg ggtagccgtg
     2881 ctcgagcgcg gcgcggttca tcaggtaacg cttggagagc ccgcacgccg agcaggtggg
     2941 ccggcgcgtg gcctgctgca cgactgggat cggggcgccc accgcctcgg ccacccgctc
     3001 gacgtgcagg gtcaggcccc gggcctccgc gaaggcctcg cacttggcgc gcgactcctt
     3061 ggagtagtcg aagatgccga gatccaggta gaggccggcg gtccggtagc cgtcctcgat
     3121 caggacgtcc cagagcgcga gcgagtcctt gccgcccgag accgcgacca tgatcgtctc
     3181 gtcggtggtg aacatgtggt gccggtggac ggcctccgcc acctgcttgc ggaagaactc
     3241 gatgaagtgg ggcgagcaga acgcggcgtt gtggcgccgc agctcgagcg aggcggcgcc
     3301 gccgcacttg cggcatttca cgcgcccgct ccgctgccgc ccgacatcac cgggcgcacc
     3361 tcgagcacgt cgtcgccgcc gaccaccgcg tcgctggtca ggagctcgtt gtcgcggatg
     3421 accagcaccg tgctgggcag gatgccgagc tcggagagca gatccttcac ccggcgcttg
     3481 ccgtccacgt ccacctcccg ccgcggattc cggaggatga ccttcacgag gcggggaatc
     3541 ccgtggccgc cttcacgtcg cggagctgct ggagatgatc cgtctcgtgg cgcacgtgca
     3601 gcagccacca ctgggcgagg tcaatctcgc cgagcgcgac gtgccgccag cgcagccccc
     3661 gcgggtccac cgcggccagg cgctccaccg actgccggct ccgctcccgg atcgtcttca
     3721 gatccgcgat cagccgctcc agcgcgtgac cgcgctccgg gaacacgacc tcgggcgccc
     3781 gggccgggcc ggggggaggc ggcggcagcg gcgagaacgc ctggaggtcg ggcggaaagg
     3841 gcggcagggg ctgcggggcg tcccggagca gcttggaggt gagcttgccg gtgccgacct
     3901 cggccagcgt caggtggtgg aggatctcgc cgaccgacca ctcgtccggg gccgggcgcc
     3961 agttgctctg cgcctggctc aacccttccg cttccttgag cagctgcgcc cggacgctct
     4021 ccatgtcgtt ccacagcgtt tccaccggag ccggccatgc cattcgtggt tccccctggg
     4081 cgacatgata caaccggaag gtgacccagc ccactccggc cgatcccggc gaatcccccc
     4141 cgggccggcc ggcctcggac ccgcccgcgt cgaatcccct cgcccggacc ccgtggcgcg
     4201 ccacgtccac gcgcccgatc taccggaatc gctggatcga cgtgcgcgag gacatcgtcg
     4261 agctgcccga cggccacacc acggtgtacg gggtcgtcga gtgcagcgag tgcgtcggcg
     4321 tcctaccgtt cctcgatccc gacacggtgc tcctggtcgg gcagtaccgc tacgtggcca
     4381 aggcgttctt ctgggagatg ccgaccggcg ggcaggacgc gggcgagacg cggctgcagg
     4441 cggcccagcg cgagctcgcg gaggaggcgg gctacgaggc cgtccgccac gtcgagctcg
     4501 gcgacttcca cacctccaag tcgatcctcc gcgaggtcgc gcacctctac ctcgcggagg
     4561 acctgcgccc ggccgcgcga cgggccgacc acaccgagtt catcgagcgc cgcgcctttc
     4621 ccttcgcgga ggtgctcgcc atggtgacgc gcggggagat caaggacgcc atgactatca
     4681 tcgccgtgct gcacgcggcg cggcggcggg ggatgtagcg gtccggctca ggacttgccg
     4741 agcaggtaca tctccccgct gggcgcgggc aggccgccgg ccggctcgag ggtcaccgcg
     4801 aaggcgttga ccgccgccac cccgggcagc ggccgcaccg cgaggctccc cgtcccggcc
     4861 gcgtcgacgg tgaagacacc cgcggagacc ggcgcgttgc cccccgcgat ggcccagagc
     4921 tggtaggcct tgccgtcggg cggcgcgggc agcccggtgg ccacgaacac cccacccgcc
     4981 tgggcgtgcc acatcatgcg ggcccgcgcc gcgggagccg gcggcaggcc ggcgagcgcg
     5041 acgacctggg tggccggatc gcgcaggatc gcgatcaccg tctgctggct gcgcagctcg
     5101 gcgcggagct gctcggcgct ctgcgcgagc gcgtcgaggc gcttctcgta ggtcgcggac
     5161 acgctccagc ccaccaccac cgcggccagg cccgcggcca tcgcgccgct cagcacgacg
     5221 ggccagagcg ggcggggccg ccgcggcagc ggccgcaccg gtgcgggggt cgccgcgatg
     5281 cgctccatca gcgccgcctt caccccgagc ggcggcacct cgggggccgc ggcggcgagc
     5341 ccgaccagcg tgtcctcgca gtcacgtagc gcgcgcagcg cgtccgggtc gcccgcctcg
     5401 agcagggcct cgaagcggac ccggtcctcg ccctcgagcc cgcccagggc gtacgcggcc
     5461 gccagctcgt cgatctcgtc gcggttcatg tggccgacag cctctcccct ccgaggaccc
     5521 cgcgcaggcg gtcgagcccg agccgggcgc gcgtcttcac cgttccgagc ggctcg
//
LOCUS       DATMCV010000393         3276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_886846, whole genome shotgun sequence.
ACCESSION   DATMCV010000393 DATMCV010000000
VERSION     DATMCV010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886846"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VNU03_09120"
     CDS             <1..294
                     /locus_tag="VNU03_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74665.1"
                     /translation="WNVGIIATGGLSHFPELSLPRVGETDTVFDRKLVHWMERGEHEP
                     LQELTVGELHKTGEHEFLNWMVLIGAITPARAQVRYFGELRRINLAAVEWSLP"
     gene            291..506
                     /locus_tag="VNU03_09125"
     CDS             291..506
                     /locus_tag="VNU03_09125"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HWP74666.1"
                     /translation="MSRYAVNSLLYRLKKEPEFRARFQADAAAALAGLDLTEAERAAF
                     VAHDMRRINELGGYLHLVMSVPGLAAH"
     gene            516..1364
                     /locus_tag="VNU03_09130"
     CDS             516..1364
                     /locus_tag="VNU03_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWP74667.1"
                     /translation="MKLVRYGRAGAERPGLVDATGRLRDLSRVVEDITPEVLTPAGLK
                     RLRAVKTDRLPAVKGKPRLGCPLASIGKIVCIGLNYTDHAQEVSLPLPKEPTIFIKAN
                     SAISGPDDAIARPPGAVKLDYEVELAAVIGRAARSVDEARALGHVAAYCLLNDVSERE
                     FQMEHGGGTTKGKSADTFAPIGPWLVTADEIADPQALALWTTVNGERRQHGSTKDMVF
                     PVRQLVAYLSRVLTLLPGDLVSTGTPAGVGHGARPPRYLVPGDTVEQGIDGLGRQRHR
                     VTQRRA"
     gene            1361..2719
                     /locus_tag="VNU03_09135"
     CDS             1361..2719
                     /locus_tag="VNU03_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP74668.1"
                     /translation="MILVEHATVITVDGERRVFADGSVLVDGARILQVGPSREVHPPR
                     EPDRVIDGSRLVVTPGFVDTHVHLSEHLNRGLLLDDIPVDRYLPDWLIPLYAVMTAED
                     EQHAALLAGIEMIRTGTTTFCEAGTLFDTSAVADAVERLGMRAILGRWTWDLESGPDR
                     MRQTTAEALAANAAMLEDLGARDGLVRGWPLLLGFGTCSEELIRGAKALADRHGVGWG
                     MMHLASHPSRRTRDRLPLATLDAWGVLAPNAKLSHMVYLDDADIARLVRRGVKISHCP
                     TAGLKHTKGLAAHARVPEMVRDGVCVSLGGDSGNGSNHFDMLRMMYLVANIYKDARLD
                     VRVMPPETVVEMATIRGAEALLMEREIGSLEVGKRADLVLFDRDAPEWRPLLNPLNNL
                     VYAASGASVRSVMIDGRLVLDEGRITTVDERAVYDRVEVLARDQVRRAGLTVLNRWPL
                     RP"
     gene            complement(2726..>3276)
                     /locus_tag="VNU03_09140"
     CDS             complement(2726..>3276)
                     /locus_tag="VNU03_09140"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWP74669.1"
                     /translation="YDLGPSAPRAKRELTPDSVISTVTAKPSGEAAPGPTVASAPAPP
                     AVALPPAPVTPRPAPVGPKAPAGPRESTPAPREAKATVAPHEAKPAPAARESKPASAA
                     AAAVAGRRTFTVQIAAFKNARQAGRVAETVKRAGYAADVLRVESSAVPWVVRIGGYST
                     REQAESARDALARKGFRGGFII"
BASE COUNT          444 a         1179 c         1223 g          430 t
ORIGIN      
        1 tggaacgtgg ggatcatcgc caccggcggc ctctcgcact tcccggagct gtcgctgccg
       61 cgggtgggcg agaccgacac cgtcttcgac cgcaagctcg tccactggat ggagcggggc
      121 gagcacgagc cgctgcagga gctgaccgtc ggcgagctgc acaagaccgg cgagcacgag
      181 ttcctcaact ggatggtgct gatcggcgcg atcaccccgg cccgggccca ggtgcgctac
      241 ttcggcgagc tgagacggat caacctcgcc gcggtggagt ggagcctccc gtgagccgct
      301 acgcggtcaa cagcctgctc taccgcctca agaaggagcc ggagttccgg gcccgcttcc
      361 aggccgacgc ggcggccgcg ctggccggcc tcgacctgac cgaggccgag cgggccgcct
      421 tcgtggcgca cgacatgcgc cggatcaacg agctgggcgg ctatctccac ctcgtgatgt
      481 cggtccccgg cctggccgcg cactagggcc cggccatgaa gctcgtccgg tatggccgcg
      541 cgggcgccga gcgcccgggg ctggtggacg cgacgggtcg cctgcgcgac ctctcgcggg
      601 tggtggagga catcaccccg gaggtgctga cgcccgccgg gctcaagcgg ctgcgggcgg
      661 tgaagacgga tcggctgccc gcggtgaagg gcaagccgcg gctgggctgc ccgctcgcct
      721 ccatcggcaa gatcgtgtgc atcgggctca actacaccga ccacgcccag gaggtctcgc
      781 tgccgctgcc gaaggagccg accatcttca tcaaggccaa cagcgcgatc agcgggcccg
      841 acgacgcgat cgcgcggccc ccgggcgcgg tcaagctcga ctacgaggtg gagctggccg
      901 cggtgatcgg gcgcgccgcg cgctcggtgg acgaggcgcg cgcgctcggc cacgtggccg
      961 cctactgcct gctgaacgac gtctccgagc gcgaattcca gatggagcac ggaggcggca
     1021 ccaccaaggg caagagcgcg gacaccttcg cgccgatcgg cccgtggctc gtcaccgccg
     1081 acgagatcgc ggatccgcag gccctcgccc tctggaccac cgtcaacggg gagcggcgcc
     1141 agcacggcag caccaaggac atggtcttcc cggtgcggca gctcgtcgcc tacctgagcc
     1201 gcgtcctgac gctgctcccc ggcgacctgg tgtcgaccgg cacgcccgca ggggtcggcc
     1261 acggggcgcg gccgccgcgc tacctcgtgc ccggcgacac ggtggagcag gggatcgacg
     1321 gcctcggacg acagcggcac cgggtgaccc agcgccgggc gtgatcctcg tcgagcacgc
     1381 caccgtcatc acggtggacg gcgagcggcg cgtcttcgcc gacggctctg tcctggtgga
     1441 cggcgcccgc atcctccagg tcggtccctc gcgcgaggtc cacccgcccc gcgagccgga
     1501 tcgcgtgatc gacggaagcc ggctggtggt gacgcccggc ttcgtggaca cccacgtgca
     1561 tctctccgag catctcaatc gcgggctgct gctcgacgac ataccggtcg accgctacct
     1621 gcccgactgg ctgatcccgc tctacgcggt gatgaccgcg gaggacgagc agcacgcggc
     1681 cctgctggcc gggatcgaga tgatccgcac cgggacgacc accttctgcg aggcgggcac
     1741 gctcttcgac acgtccgcgg tggcggacgc ggtcgagcgg ctcgggatgc gcgcgatcct
     1801 gggccgctgg acgtgggatc tggagtcggg tcccgaccgc atgcggcaga ccaccgcgga
     1861 ggcgctcgcg gccaacgcgg cgatgctcga ggacctgggc gcgcgcgacg gcctcgtgcg
     1921 cggctggccg ctcctgctcg gcttcggcac ctgctcggag gagctgatcc gcggggccaa
     1981 ggcgctggcc gatcggcacg gggtggggtg ggggatgatg cacctggcct cgcatccctc
     2041 gcgccgcacc cgggaccgcc tgccgctcgc gacgctcgac gcctggggcg tgctcgcgcc
     2101 gaacgccaag ctctcccaca tggtctatct cgacgacgcg gacatcgcgc ggctcgtgcg
     2161 gcgcggggtg aagatcagcc actgtccgac cgcggggctc aagcacacca aggggctcgc
     2221 cgcgcacgcg cgcgtcccgg agatggtccg ggacggggtc tgcgtctcgc tgggcggcga
     2281 cagcggcaac gggtcgaatc acttcgacat gctgcggatg atgtatctcg tcgccaacat
     2341 ctacaaggac gcgcggctcg acgtgcgggt gatgccgccc gagacggtgg tcgagatggc
     2401 gacgatccgc ggagcggagg cgctcctgat ggagcgggag atcggctcgc tcgaggtggg
     2461 caagcgcgcc gatctcgtgc tcttcgaccg cgacgcgccg gagtggcgcc cgctgctcaa
     2521 cccgctcaac aacctggtct acgcggcgag cggggcgagc gtgcgcagcg tgatgatcga
     2581 cgggcggctc gtgctggacg aggggcggat caccaccgtc gacgagcggg ccgtctacga
     2641 tcgagtggaa gtgctggcgc gcgaccaggt gcgccgcgcc gggctcaccg tcctgaaccg
     2701 gtggccgctc cggccctgac cgggcctaga tgatgaagcc gccccggaag cccttccggg
     2761 cgagcgcgtc gcgggccgac tcggcctgct cgcgcgtcga gtacccgccg atccgcacca
     2821 cccacggcac cgcggaggac tccacccgga gtacgtcggc cgcgtagccc gcgcgcttga
     2881 ccgtctcggc cacgcgcccg gcctgccggg cattcttgaa ggcggcgatc tgcaccgtga
     2941 acgtccgccg gccggccacc gccgccgcgg cggcgctcgc gggcttgctc tcgcgcgcgg
     3001 ccggcgcggg cttcgcctcg tgcggggcga ccgtcgcctt cgcctcgcgg ggcgcgggcg
     3061 tgctctcgcg tgggccggcc ggggccttcg gcccgaccgg ggccgggcgc ggcgtcaccg
     3121 gggcgggcgg aagcgcgacg gccggcggcg cgggggcgga cgcgaccgtc gggccggggg
     3181 cggcctcgcc cgagggcttg gcggtgacgg tcgagatcac cgaatccggg gtcagctcgc
     3241 gcttggcccg cggggccgag ggcccgaggt cgtacc
//
LOCUS       DATMCV010000394         2450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_354875, whole genome shotgun sequence.
ACCESSION   DATMCV010000394 DATMCV010000000
VERSION     DATMCV010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354875"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VNU03_09145"
     CDS             complement(<1..282)
                     /locus_tag="VNU03_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP74670.1"
                     /translation="MTLEHLDVGGIPLDILRRGAGRPLLVLHGEAPLDPGAPVVDLLA
                     THAEVIAPTHPGFGHSARPDDFDTVYDLVHLYLDLLEHLPPGPVTLMGLS"
     gene            complement(282..1001)
                     /locus_tag="VNU03_09150"
     CDS             complement(282..1001)
                     /locus_tag="VNU03_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74671.1"
                     /translation="MASDALELHDIEAFYGDSHVLHRMSLRLGEGRLLGLLGRNGAGK
                     STSMSVTVGLLPPRHGRVAVFGRAVTGRAPEAIAAQGVALVPQGRRIFRSLTVRENLA
                     VAGRRPRGGAATSWTLDAVYALFPRIGERRRQVAGSLSGGEQQMLAIGRALMSNPRVL
                     LMDEPSEGLAPQLVAEVMATIRRLKEQGLSILLVEQNPKLVFQVADDIVILNGGRVVA
                     EGPAAEFRHRDVDLRQHLGIF"
     gene            complement(991..1740)
                     /locus_tag="VNU03_09155"
     CDS             complement(991..1740)
                     /locus_tag="VNU03_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74672.1"
                     /translation="MIEPATALSVQGLTKSFGGLLVTASVDLTVEAGERRLIIGPNGA
                     GKTTLFNLVTGELRPDGGTVTLFGRDITRIPGWHRAHLGISRTYQIITLFPRETLLRN
                     VTLSLLGRSPLRWNPVARLERQTALMGRAREALARVGLGHLAERPLSQTSYGERRRVE
                     IAMALAQAPRILLLDEPFAGLSIEERRDVLQLVTRIPRDVTIVMIEHDMDVALDFAER
                     ITVLHFGHVVVEGTRGEVVAHPRTREIYLGE"
     gene            complement(1737..>2450)
                     /locus_tag="VNU03_09160"
     CDS             complement(1737..>2450)
                     /locus_tag="VNU03_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74673.1"
                     /translation="ALFATLALRGSGSGIGFVMITLAIGQIVWGVAYRWISITNGDNG
                     ISVSSRPSPLGLSLAAPAHFYWATLVVFLVALGSIAMLVASPFGASLRGTRDQPRRMA
                     ALGYHVWMIRFLAFLLSGFWSGVAGLLFLYYNQFVSPQAVALTASAEALLMVIAGGSG
                     TLLGPVVGAGLVVVMKNVASAYIERWNFVLGAIFVAIVVFMPEGLVPGAARLWRRATA
                     RPGAPAVAPVPAPGEGPAQ"
BASE COUNT          388 a          872 c          895 g          295 t
ORIGIN      
        1 cgacaggccc atcagggtca ccggcccggg aggcaggtgc tcgagcaggt cgagatagag
       61 gtgcaccagg tcgtagaccg tgtcgaagtc gtcggggcgg gccgagtgcc cgaaccccgg
      121 gtgcgtgggt gcgatgacct cggcgtgggt cgcgagcagg tcgaccaccg gcgcgccggg
      181 gtcgagcggc gcctcgccgt ggagcacgag gagcggccgc ccggccccgc gtcgcaggat
      241 gtcgagcggg atgccgccca cgtccaggtg ctcgagggtc atcagaagat tcccaggtgc
      301 tggcgtaggt ccacgtcccg atgccggaac tccgcggcgg gcccttcggc cacgacgcgg
      361 ccgccgttga ggatgacgat gtcgtcggcg acctggaaca ccagcttggg attctgctcg
      421 accagcaaga tcgacaaccc ctgctccttg agccgccgga tcgtcgccat gacctcggcg
      481 acgagctgag gcgcgaggcc ctccgaaggc tcgtccatca gcagcacccg gggattggac
      541 atcagggccc ggccgatggc gagcatctgc tgctcgccac ccgagagcga gcccgcgacc
      601 tgccgccggc gctccccgat gcgcgggaag agcgcgtaga ccgcgtcgag cgtccagctc
      661 gtcgcggcgc cgccccgggg ccgacggccc gccaccgcga ggttctcgcg cacggtgagg
      721 ctccggaaga tccggcgccc ctgcggcacc agcgccacgc cctgggccgc gatggcctcg
      781 ggcgcgcgac cggtcaccgc ccggccgaag accgcgacgc ggccgtgacg ggggggcagg
      841 agcccgacgg tcacgctcat ggaggtggac ttgccggcgc cgttgcggcc gagcaatccg
      901 aggagccggc cctcgccgag gcggagcgac atccggtgca gcacgtggct gtcaccgtag
      961 aacgcctcga tgtcgtggag ctccagcgcg tcactcgcca agatagatct cccgggtccg
     1021 cgggtgcgcc accacctcgc cgcgcgtgcc ctcgaccacc acgtggccga agtgcagcac
     1081 ggtgatccgc tccgcgaagt ccagcgccac gtccatgtcg tgctcgatca tcacgatcgt
     1141 gacatcccga ggtatgcgtg tcaccagctg caggacgtcg cggcgctcct cgatggagag
     1201 cccggcgaag ggctcgtcca gtaacaggat ccggggggcc tgggccaggg ccatggcgat
     1261 ctccacgcgg cgccgctccc cgtaggacgt ctgcgagagc gggcgctccg ccaggtgccc
     1321 gagcccgacg cgggccagcg cctcgcgcgc ccggcccatc agcgcggtct ggcgctccag
     1381 ccgggcaacc ggattccagc ggagcggcga gcgcccgaga agcgagaggg tgacgttccg
     1441 cagcagcgtc tcgcgcggga acagcgtgat gatctggtag gtgcgggaga tcccgagatg
     1501 cgcgcggtgc cacccgggga tccgggtgat gtcccggccg aagagcgtga ccgtcccgcc
     1561 gtcggggcgg agctcgccgg tcaccaggtt gaagagcgtg gtcttgcccg cgccgttcgg
     1621 gccgatgatc aggcgccgct cccccgcctc gacggtgaga tccaccgaag cggtgacgag
     1681 caggccgccg aaggacttcg tcaggccctg taccgacagg gcggtcgcgg gctcgatcac
     1741 tgggccggcc cctcgcccgg cgcgggcacc ggcgccaccg ccggggcccc gggccgcgcc
     1801 gtcgcccgcc gccatagccg ggccgcgccg ggcaccaggc cttccggcat gaagaccacg
     1861 atcgccacga agatggcgcc gaggacgaag ttccagcgct cgatgtaggc gctcgcgacg
     1921 ttcttcatga ccaccacgag gccggcgccg accaccgggc ccagcagggt cccgctcccg
     1981 cccgcgatca ccatcaggag cgcctcggcc gaggcggtga gcgccaccgc ctgcgggctc
     2041 acgaactggt tgtagtagag gaagagcagg ccggccactc cgctccagaa gcccgagagg
     2101 aggaacgcga ggaaccggat catccagacg tggtagccga gcgcggccat gcgccgcggc
     2161 tggtcgcgcg tgccgcgaag gctcgcgccg aacggcgagg ccaccagcat cgcgatggag
     2221 cccagggcga cgaggaagac cacgagggtc gcccagtaga agtgggccgg cgcggccagc
     2281 gacagaccga gcgggctcgg ccgcgagctc accgagatgc cgttgtcccc gttcgtgatg
     2341 ctgatccagc gatacgcgac gccccagacg atctggccga tcgccagggt gatcatgacg
     2401 aagccgatgc cgctgccgct gccgcgcagc gcgagggtcg cgaagagggc
//
LOCUS       DATMCV010000395        10517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_683731, whole genome shotgun sequence.
ACCESSION   DATMCV010000395 DATMCV010000000
VERSION     DATMCV010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683731"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VNU03_09165"
     CDS             <1..1255
                     /locus_tag="VNU03_09165"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP74674.1"
                     /translation="PPLGAYRGVGMTMGAFVMERTLDLLADRLGLDPALVRQRNLIPR
                     DAYPFTSATGFVYDSGDYPKALEAALTLAGYERLVREREAGRARGRLLGVGLACYTEY
                     TGIGSETYRRRGMVDMPGPEAATVRVEADGAVTCLVSFPSQGQGHATTTAQLVADQLG
                     VPLEAVTVRQPDTDEAPPGTGTFASRGAIAQSGAADAAAATVRGALLAAAGGLLEASP
                     ADLVLADGMVHVRGVPDRGMTVAEVARRAHATHGGGARLAATQRFDPPGPTFSGAVHV
                     ASVEVDPETGRVTVREYAVVEDCGPVINPMIVEGQIHGAVAQGIGEALGERLVYDESG
                     QLLTGTLMDYPLPTAAMLPAFTIGHLETPSPLTPGGFKGMGEGGTIGAPAAIANAVAD
                     AVKPLGVAVTGLPILSADLVRRHGS"
     gene            1245..3275
                     /locus_tag="VNU03_09170"
     CDS             1245..3275
                     /locus_tag="VNU03_09170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWP74675.1"
                     /translation="MDPSEPAVPSDVAVTGAARSGPRALWWIVAQVGVLALAAGLRLW
                     RLDQNGFDNEYYAAAVRSMMASWHAFFYNSFDPAGFVSVDKPPLALWIQVASVKLFGF
                     RPSAVLLPQVIEGVAAVAVLSHLVRRHWGAAAGLLAGLFLALTPIAVAVDRSGNTDTG
                     LVLVVLLAAWALLLAAERGHRGFLLLAMALIGLGFNVKMLAAFVVLPTFAAVYWLGAA
                     APWRRRLVDLALGGLVLAAVSLPWMIAYDLTPAARRPFVGSSPQNSMIDLAVGHNGVG
                     RFVRLWQSGRTASADETAARRRASAPPAAGPAEGYARLFVRAPVGPLRLGDGLLAAQA
                     EWLLPLALVGAVALGRGGARPPLRPAQLGLLLWIGWTLTYAVVYSAAGGIFHFYYLST
                     LGPPLAALAATGVIDLWQRYRDGRAAAVALAGALLVTAMWQVYVHTAGLPAVSDEWQR
                     RMPIGALLAVVLAAAVLLARLLRRGERPGVGISERIAVAVGLGALLVMPAAWALSSVL
                     VRGVAVIPSADVGRLRSPSPTPTLRARVRVGEVVDRRQLIAFLEANRHGERFLLATPS
                     AQLASPLIVATGLPVMAMGGFHGLDPILTPERLAGLVAAGEVRFVMLGELSIASRLMG
                     AESAGRPLAAWIRANGTPVDPALWSESPGVPGRAWRRELFDLKPGTGLVPAS"
     gene            3289..4089
                     /locus_tag="VNU03_09175"
     CDS             3289..4089
                     /locus_tag="VNU03_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWP74676.1"
                     /translation="MGIGRAHVRGTRWWLAMGLALSAGLGGAAATPARADEIKVWSAG
                     AVRAIVTELSEAFRQETGHTVALSFGTVGVTRGKIASGEPVDVVIMTDAAVDDVIREG
                     ALVAGSRADLARTGMGVGVREGAPRPDISTPEAFKATLLAAKSLVYVDPAQGATSGIH
                     FKGVLERLGIADAVRAKTQLVAGGYPAEKVASGEAEIVVHQISEIVPVKGVVMIGPLP
                     AELQKVTVYSAGLAARTTHEATARAFMGFLVRPAFRAKFAAAGLDYKP"
     gene            4151..4999
                     /gene="lgt"
                     /locus_tag="VNU03_09180"
     CDS             4151..4999
                     /gene="lgt"
                     /locus_tag="VNU03_09180"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWP74677.1"
                     /translation="MFASPGAIAFHLGPLTVRWYGILMATAILVGFWLAQRRATEEGL
                     PADEILRAAQWAVVAGLIGARLYEVIFNWDYYGRFPEKIVAVWEGGLAMHGGLIVGPL
                     VGIWLARRWGVPIRRALDVIAPSMILGQAIGRWGNFFNEEAFGAPTDLPWKLYISPLH
                     RPPELRDVEYFHPTFLYESLWDLLVFVLLVWWVRPRVARRPGAVFFAYIGLYSVGRFF
                     IEALRLDSFWVGSLRVPQLASVAGVLVAAAGLLWVLRGAPVEPAAPVREPTGKAPATR
                     GRRPRG"
     gene            complement(4953..6233)
                     /locus_tag="VNU03_09185"
     CDS             complement(4953..6233)
                     /locus_tag="VNU03_09185"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWP74678.1"
                     /translation="MTDATDPTDSPEFDSELHRTALAQLDRVAARLSLDHDIHERLRF
                     PRRALVVSVPVRMDSGKTQVFIGYRVHHNTALGPTKGGLRYDLGVNLGEVTALAMLMS
                     WKCALVGLPYGGAKGGIRCNPRAMSAHEREHLTRRYTTEIILMIGPDIDIPAPDLGTD
                     GQTMAWMMDTYSMTQGKSVPGVVTGKPLIVGGSVGRREATGRGIVFVLYQAAKSLGQE
                     LRGKKIVVQGFGNVGGTAARLLWNDGCTIAGISDFKGGVWNPNGLDVRQLQAYVAETG
                     SVTGFPGGEAIGNDQILEQPCDVLIPAAIDSVINERNADRIKALIVAEGANGPTTPEA
                     DVILRDRGVTVIPDILCNAGGVVVSYFEWVQGLQHYFWKESEIISRLQEIMARAYTRV
                     WQTAQKGGVDLRTGALMEGVSRVADAHVSRGLYP"
     gene            complement(6236..8401)
                     /gene="glgX"
                     /locus_tag="VNU03_09190"
     CDS             complement(6236..8401)
                     /gene="glgX"
                     /locus_tag="VNU03_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWP74679.1"
                     /translation="MSEDLPRTRPGRPYPLGATWDGGGVNFALFSEHATAVALCFYAE
                     RDPGKEIRQIRIEQRTDQVWHVYVPGIRPGTLYAFRVNGPYEPRQGHRFNPAKALLDP
                     YAKAIAGSLQWNDAFFGYRTGDPEGDVQPDDRDSAPYLPKSVVVDPTFDWEGDRPPLT
                     RWSRTVIYEVHVKGFTRLHPEVPPELRGTYAGLAAPAAVRHLTELGVTAVELLPVHHS
                     VTEKYQADRGLANYWGYNSIGFFCPDSRYSAAGHVGQQVDEFKQMVKTLHRAGIEVIL
                     DVVYNHTAEGNHLGPTLSFRGIDNEAYYRLSPEDQRYHVDYTGTGNTLNMRHPRTIQL
                     MMDSLRYWVLDMHVDGFRFDLAAALARELHDVDRLSAFFDVIHQDPVISQVKLIAEPW
                     DLGEGGYQVGNFPVGWAEWNGKYRDTIRSYWKGDAGQVAELAYRLTGSSDLYEMGGRR
                     PYASVNFVTAHDGFTLADLVSYEAKHNLANGEDNRDGSDDNRSWNCGVEGPTRDPKIV
                     ALRERQMRNFLATLFLSQGIPMLLAGDEIGRTQRGNNNAYCQDNELSWLAWPPTETGR
                     RLIDFTRRLIRLKHENPVFHRRTFFQGRRIQGSAVKDLAWLRPDGHEMTDEEWPDNQS
                     RCLGLRLAGDAIEEVDDMGEPIVGDTFLILLNADHEPVPFVLPAHEARVLWEPVLDTR
                     DWDGAADRPPQRTGEHYRLEGRSLAVLRLHRNPRPGEGA"
     gene            complement(8415..>10517)
                     /gene="glgB"
                     /locus_tag="VNU03_09195"
     CDS             complement(8415..>10517)
                     /gene="glgB"
                     /locus_tag="VNU03_09195"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWP74680.1"
                     /translation="EGMVSVRALVPTARAVAAVMPDGTVSPLRAVHPAGFWEGEVPGR
                     RLPLAYRLRVVDEQGQVSEVEDPYRFPPTLSAYDLHLLGEGTHYRLFERLGAHPIRHE
                     GVEGVRFVVWAPNARRVSVVGDWNGWDGRRQPMRLHPGNGLWELFVPGVTLGARYKYE
                     IIGRDGGLLALKTDPVAFAFEPDEPRTASAVTDLNTYTWGDAEWMDERKRRNALHRPM
                     SVYEVHLGSWRRPEGGTLPSYREVAEQLADYVTELGFTHVELLPVTEHPYYASWGYQT
                     LGYYAPTRRYGTPTDFMAFVDTLHRRGIGVIMDWVPAHFPKDAHGLAFFDGTHLYEHD
                     DPNLRDHPDWGTRVFNFGRREVSNFLIANAVFWLERYHIDGLRVDAVASMIYRDYSRK
                     AGEWIPNEFGGRENLEALAFLKKLNEVVYGGQPGASTIAEESTAWPQVSRPVYLGGLG
                     FGFKWNMGWMHDILEYMQHQPVHRKYHHNQLTFGLLYAWTENFVLPLSHDEVVYGKRS
                     LAHKMPGDDWQRFANLRLLYAFMWAYPGKKLLFMGGEFGQSDEWNHDHALDWWLLDTG
                     PFHRGVQQLVKDLNRVYRSDPSLYEVDFEPAGFQWIDCSDWEQSVVTFCRFSRRQERL
                     LLCACNFTPVARTGYRVGVPRPGYYREILNTDAALYGGGDVGNSGGVSSEPTPWHGQP
                     HSVVLTIPPLAAVWLVPA"
BASE COUNT         1321 a         3622 c         3953 g         1621 t
ORIGIN      
        1 gccgcccctc ggcgcctacc gcggggtggg catgaccatg ggcgccttcg tgatggagcg
       61 caccctcgac ctgctcgcgg accggctcgg gctcgatccc gccctcgtcc gccagcgcaa
      121 cctgatcccg cgcgacgcct acccgttcac ctccgccacc ggcttcgtct acgacagcgg
      181 ggattatccc aaggcgctcg aggccgcgct gaccctggcc ggctacgagc ggctggtgcg
      241 cgagcgcgag gccgggcgcg cgcgcgggcg gctgctcggg gtcgggctgg cgtgctacac
      301 cgagtacacc gggatcggct ccgagaccta tcgccggcgc gggatggtcg acatgccggg
      361 gccggaggcg gcgacggttc gcgtggaggc cgacggggca gtgacctgcc tcgtgtcgtt
      421 tccgtcgcag ggccaggggc acgccaccac caccgcgcag ctcgtggccg accagctcgg
      481 cgtgccgctc gaggcggtga cggtgcggca gcccgacacc gacgaggcgc cgcccggcac
      541 cggcaccttc gcgagccggg gcgccatcgc gcagagcggc gcggccgacg cggcggcggc
      601 caccgtgcgc ggcgcgctgc tggccgcggc gggcgggctg ctcgaggcga gcccggcgga
      661 cctggtgctg gccgacggca tggtgcacgt gcgcggcgtg cccgatcgcg ggatgacggt
      721 ggcggaggtg gcgcgtcggg ctcacgcgac ccacggcggg ggcgcacggc tcgccgcgac
      781 ccagcgcttc gatcccccgg ggcccacgtt ctccggcgcg gttcacgtcg cgtcggtgga
      841 ggtcgatccg gagaccggcc gggtcacggt gcgcgagtac gcggtggtcg aggactgcgg
      901 gccggtgatc aacccgatga tcgtcgaggg gcagatccac ggcgcggtcg cccaggggat
      961 cggcgaggcg ctgggcgagc ggctggtata cgacgagagc ggccagctcc tgaccggcac
     1021 cctgatggac taccccctgc cgaccgcggc gatgctgccc gcgttcacga tcggccatct
     1081 cgagacgccc tcgccgctca ctcccggcgg cttcaagggc atgggcgagg gcggcaccat
     1141 cggcgcgccc gccgccatcg ccaatgcggt ggccgacgcg gtcaagccgc tcggggtggc
     1201 ggtgaccggg ctgccgatcc tgtccgccga tctcgttcga cgccatggat cctagcgagc
     1261 ccgccgttcc atccgacgtc gcggtcaccg gggccgcgcg gtcagggccg cgagcgctct
     1321 ggtggatcgt cgcccaggtc ggggtgctcg cgctggccgc gggcctgcgc ctctggcggc
     1381 tcgaccagaa cgggttcgac aacgagtact acgcggccgc ggtgcgcagc atgatggcga
     1441 gctggcacgc gttcttctac aactccttcg acccggccgg cttcgtctcg gtcgacaagc
     1501 cgccgctcgc gctgtggatc caggtggcga gcgtgaagct cttcggcttc cggccgtccg
     1561 cggtgctgct gccgcaggtg atcgagggcg tggccgcggt ggcggtgctc tcgcacctgg
     1621 tgcggcgtca ctggggcgcc gccgcgggcc tgctggccgg gctcttcctg gctctcacgc
     1681 cgatcgcggt cgcggtggac cgctccggaa acacggacac cggcctcgtg ctggtggtgc
     1741 tgctggcggc ctgggcgctc ctgctcgccg ccgagcgcgg tcaccgcggt ttcctgctgc
     1801 tcgccatggc cctgatcggg ctcggcttca acgtgaagat gctggccgcg ttcgtggtcc
     1861 tgccgacgtt cgcggcggtc tactggctgg gcgcggccgc gccatggcgg cgtcgcctgg
     1921 tcgacctggc gctcgggggt ctcgtgctcg ccgcggtgtc gctgccgtgg atgatcgcct
     1981 acgatctcac ccccgccgcc cggcgcccgt tcgtgggcag cagcccgcag aactccatga
     2041 tcgatctcgc ggtgggccac aacggcgtcg gtcggttcgt gcggctctgg cagtccggac
     2101 ggacggcgag tgcggacgag acggcggcgc ggcggcgggc gagcgcgccc ccggccgcgg
     2161 gcccggccga agggtacgcg cggctcttcg tgcgcgcgcc ggtgggcccg ctgcgcctgg
     2221 gcgacggcct gctcgcggcg caggcggagt ggctgctgcc gctggccctg gtgggcgcag
     2281 tcgcgctcgg gcgagggggc gcgcggccgc cgctccggcc cgcgcagctc ggcctgctcc
     2341 tctggatcgg ctggacgctc acctacgcgg tcgtctacag cgcggcgggc gggatctttc
     2401 acttctacta cctctccacg ctcgggcccc cgctggccgc gctggccgcg accggcgtga
     2461 tcgacctgtg gcagcgctac cgcgacggcc gcgcggccgc ggtggcgctc gcgggtgccc
     2521 tcctggtcac cgcgatgtgg caggtctacg tgcacaccgc cgggctcccc gcggtctccg
     2581 acgagtggca gcgccggatg ccgatcggcg cgctcctcgc ggtggtgctc gcggccgcgg
     2641 tgctcctggc gcgcttgctc cgccgcggcg agcggccggg agtcggtatc tccgagcgca
     2701 tcgcggtggc tgtcgggctc ggcgcgctgc tggtgatgcc ggcggcgtgg gcgctcagca
     2761 gcgtgctggt gcgcggggtg gcggtgatcc cgtccgccga cgtcggccgg ctccgctcac
     2821 cgtcgccgac gcccaccctg cgggcccgcg tccgcgtggg cgaggtcgtc gatcgccgtc
     2881 agctgatcgc gttcctcgag gccaaccgcc acggcgagcg cttcctgctc gccaccccga
     2941 gcgcgcagct ggcctccccg ctcatcgtcg ccaccggcct gccggtgatg gcgatgggcg
     3001 gcttccacgg cctcgacccc atcctgaccc ccgagcgcct ggcggggctc gtggccgcgg
     3061 gggaggtgcg cttcgtgatg ctgggcgagc tctcgatcgc gagccgcctc atgggcgcgg
     3121 agtcggcggg gcgtccgctc gcggcctgga tccgcgccaa cgggacgccg gtggatccgg
     3181 cgctctggag cgagtcgccc ggcgtccccg gccgggcctg gcggcgggag ctcttcgacc
     3241 tgaaaccggg cacgggactc gtcccggcct cctgagagga gacgacgcat gggtatcgga
     3301 cgcgcgcacg ttcgcggtac gcggtggtgg ctcgcgatgg ggctggcgct gtcggccggg
     3361 ctgggcgggg ccgcggccac gcccgctcgc gccgacgaga tcaaggtgtg gagcgcgggc
     3421 gcggtgcgcg cgatcgtgac cgagctctcg gaggccttcc ggcaggagac cgggcacacg
     3481 gtggcgctgt cgttcggcac cgtcggggtc acgcgcggca agatcgcgag cggggagccg
     3541 gtggacgtgg tcatcatgac cgacgcggcg gtggacgacg tgatccgcga gggcgcgctg
     3601 gtcgcgggta gccgcgccga cctcgcccgc accgggatgg gagtcggcgt ccgcgagggc
     3661 gcgccgcgcc cggacatctc gacgccggag gccttcaagg cgaccctgct cgccgcgaag
     3721 tcgctggtct acgtcgaccc cgcgcagggg gccacgagcg gcatccactt caagggcgtg
     3781 ctcgagcgcc tcggcatcgc ggacgcggtc cgggccaaga cccagctggt ggcgggcggc
     3841 tatcccgccg agaaggtggc gagcggcgag gccgagatcg tggtgcacca gatcagcgag
     3901 atcgtcccgg tcaagggcgt ggtgatgatc gggccgctgc ccgcggagtt gcagaaggtc
     3961 accgtctact cggcgggcct ggccgcgcgc accacgcatg aggcgaccgc gcgcgccttc
     4021 atggggttcc tggtgcggcc ggccttcagg gcgaagttcg ccgcggcggg gctcgactac
     4081 aagccctgag cgatcccggc ggcgctcgtc gcgggcgatg cgccgccatc gccgtggtat
     4141 cgtgctctcc gtgttcgcct cgcccggcgc catcgccttc catctcgggc cgctcacggt
     4201 ccgctggtac ggcatcctca tggccaccgc gatcctggtc ggcttctggc tcgcccagcg
     4261 acgcgcgacc gaggaaggtc tccccgcgga cgagatcctg cgcgcggccc agtgggcggt
     4321 ggtggccggg ctgatcggcg cgcgcctcta cgaggtgatc ttcaactggg actactacgg
     4381 ccgcttcccg gagaagatcg tcgcggtgtg ggaaggcggc ctggcgatgc acggcggcct
     4441 catcgtgggg ccgctggtcg ggatctggct cgcgcgccgc tggggggtgc cgatccggcg
     4501 cgcgctcgac gtcatcgcgc cgagcatgat cctggggcag gccatcggcc gctgggggaa
     4561 cttcttcaac gaggaggcct tcggcgcgcc gaccgatctc ccgtggaagc tctacatctc
     4621 gccgctccac cggccgcccg agctgcgcga cgtcgagtac ttccacccga cgtttctcta
     4681 cgagtcgctg tgggacctgc tcgtcttcgt cctgctcgtc tggtgggtgc gcccgcgggt
     4741 cgcccggcgg ccgggcgcgg tcttcttcgc ctacatcggg ctctactcgg tggggcggtt
     4801 cttcatcgag gcgctgcggc tcgacagctt ctgggtgggc tcgctgcggg tgccgcagct
     4861 cgcgagcgtg gccggcgtgc tggtcgcggc ggcggggctg ctctgggtgc tgcgcggcgc
     4921 cccggtcgag ccggcggcgc cggtgcggga gcctacgggt aaagcccccg cgacacgtgg
     4981 gcgtcggcca cgcggctgac gccttccatc agcgcaccgg tccggagatc caccccgccc
     5041 ttctgagcgg tctgccacac ccgggtgtac gcgcgcgcca tgatctcctg caggcgcgag
     5101 atgatctcgc tctccttcca gaagtagtgc tgcagaccct ggacccactc gaagtacgag
     5161 acgaccaccc cacccgcatt gcacaggatg tcggggatca cggtcacccc gcggtcgcgc
     5221 aggatcacgt cggcctcggg ggtggtggga ccgttggcgc cctcggccac gatgagggcc
     5281 ttgatgcggt cggcgttgcg ctcgttgatc accgagtcga tcgcggcggg gatcagcacg
     5341 tcacagggct gctcgaggat ctggtcgttg ccgatggcct cgccccccgg gaagccggtc
     5401 acgctcccgg tctcggccac gtaggcctgg agctggcgca cgtcgaggcc gttcggattc
     5461 cacaccccgc ccttgaagtc gctgattccc gcgatggtgc acccgtcgtt ccacagcagg
     5521 cgcgcggcgg tcccgcccac gttgccgaag ccctgcacca cgatcttctt gccccgcagc
     5581 tcctgcccga ggctcttggc cgcctggtag agcacgaaga cgatgccgcg gccggtggcc
     5641 tcgcggcggc cgaccgagcc gcccacgatc aagggcttgc cggtgaccac cccggggacg
     5701 ctcttgccct gggtcatcga gtaggtgtcc atcatccagg ccatggtctg cccgtcggtg
     5761 ccgaggtcgg gcgccgggat gtcgatgtcc ggcccgatca tgaggatgat ctcggtggtg
     5821 tagcggcggg tgaggtgctc gcgctcgtgg gcggacatcg cgcgcggatt gcagcggatc
     5881 ccgcccttgg cgcccccgta gggcagcccc accagcgcgc acttccagct catcagcatc
     5941 gcgagcgcgg tcacctcgcc gaggttcacc ccgaggtcgt agcgcaggcc gcccttggtg
     6001 ggaccgagcg cggtgttgtg gtggacgcgg tagccgatga agacctgggt cttcccgctg
     6061 tccatgcgca ccggcaccga gaccaccagg gcgcgccgcg ggaagcgcag tcgctcgtgg
     6121 atgtcgtgat cgaggctcag gcgcgccgcc acccggtcga gctgggccag cgcggtgcgg
     6181 tgcagctcgg agtcgaactc gggcgagtcg gtggggtcgg tcgcatcggt catggtcagg
     6241 ctccttcgcc tgggcgggga ttccggtgca ggcgcagcac cgcgagcgag cggccctcga
     6301 gccggtagtg ctcgccggtg cgctggggcg gccgatcggc cgcgccgtcc cagtcgcggg
     6361 tgtcgagcac cggctcccag agcacgcgcg cctcgtgggc gggcagcacg aacgggaccg
     6421 gctcgtgatc ggcgttgagg aggatcagga aggtgtcgcc cacgatgggc tcgcccatgt
     6481 cgtccacctc ctcgatggcg tcgcccgcga ggcgcaggcc gaggcagcga ctctggttgt
     6541 cgggccactc ctcgtcggtc atctcgtgac cgtccggccg gagccaggcg aggtccttca
     6601 ccgccgagcc ctggatgcgg cggccctgga agaacgtgcg ccggtggaac accgggttct
     6661 cgtgcttgag ccggatcagg cggcgcgtga agtcgatgag gcgccggccg gtctcggtgg
     6721 gcggccacgc gagccacgac agctcgttgt cctggcagta ggcgttgttg ttgccgcgct
     6781 gggtgcggcc gatctcgtcg ccggccagca gcatcgggat gccctgggac aggaagaggg
     6841 tggccaggaa gttgcgcatc tgccgctcgc ggagggccac gatcttgggg tcgcgggtcg
     6901 ggccctcgac gccgcagttc cacgagcggt tgtcgtcgct gccgtcgcga ttgtcctcgc
     6961 cgttggccag gttgtgcttg gcctcgtacg agaccaggtc cgcgagggtg aagccgtcgt
     7021 gggcggtgac gaagttgacc gacgcgtacg ggcgccggcc gcccatctcg tagaggtcgc
     7081 tcgagccggt gaggcggtag gccagctccg ccacctggcc cgcgtcgccc ttccagtagc
     7141 tgcggatggt gtcgcggtac ttgccgttcc actccgccca gcccaccggg aaattgccga
     7201 cctggtagcc gccctcgccc aggtcccacg gctccgcgat cagcttgacc tgcgagatca
     7261 ccggatcctg gtggatcacg tcgaagaagg cggagaggcg atccacgtcg tgcagctcgc
     7321 gggcgagggc ggccgcgagg tcgaagcgga agccgtccac gtgcatgtcg agcacccagt
     7381 agcgcaggct gtccatcatg agctggatgg tgcgcgggtg ccgcatgttc agcgtgttgc
     7441 cggtcccggt gtagtcgacg tggtagcgct ggtcctcggg ggagagacgg tagtaggcct
     7501 cgttgtcgat gccgcgaaag gagagcgtgg gcccgaggtg attgccctcc gcggtgtggt
     7561 tgtagaccac gtcgaggatc acctcgatgc cggcccggtg gagcgtcttc accatctgct
     7621 tgaactcgtc cacctgctgg ccgacgtgcc cggcggccga gtagcgcgag tcggggcaga
     7681 agaagccgat cgagttgtag ccccagtagt tggcgaggcc gcggtcggcc tggtacttct
     7741 cggtcaccga gtgatggacg ggcagcagct ccaccgcggt cacgcccagc tcggtgaggt
     7801 gacggaccgc cgcgggcgcg gcgaggcccg cgtaggtgcc gcgcagctcc gggggcacct
     7861 cgggatggag ccgggtgaag cccttgacgt gcacctcgta gatgacggtg cgcgaccagc
     7921 gcgtcagcgg aggccggtcg ccttcccagt cgaaggtggg atcgaccacc acgctcttgg
     7981 gcagataggg ggcactgtcc cggtcgtcgg gctggacgtc accctcgggg tcgccggtcc
     8041 ggtagccgaa gaacgcatcg ttccactgga gcgagcccgc gatggccttc gcgtagggat
     8101 cgagcagggc cttggccggg ttgaagcggt gcccctgtcg gggctcgtag gggccgttga
     8161 cccggaaggc gtagagggtg ccgggtctta tccccgggac gtagacgtgc cacacctgat
     8221 cggtgcgctg ctcgatccgg atctggcgga tctccttgcc gggatcgcgc tcggcgtaga
     8281 agcagagggc caccgcggtg gcgtgctcgc tgaagagggc gaagttgacg ccgccgccgt
     8341 cccaggtggc gccgagtgga taggggcggc ccgggcgggt gcgcgggagg tcctcgctca
     8401 tgcccgccgc gccctcaggc ggggacgagc cagaccgcgg cgagcggcgg gatcgtcagc
     8461 accaccgagt gcggctggcc gtgccacggg gtgggctcgc tcgacacgcc gcccgagttg
     8521 cccacgtcgc cgccgccgta gagcgccgcg tcggtgttga ggatctcgcg gtagtagccg
     8581 gggcggggga ccccgacccg gtagccggtg cgggccaccg gggtgaagtt gcacgcgcac
     8641 aggaggagcc gctcctgccg gcgggagaaa cggcagaacg tgaccacgct ctgctcccag
     8701 tcgctgcagt cgatccactg gaagccggcc ggctcgaagt ccacctcgta gagcgacgga
     8761 tcgctccggt agacgcggtt caggtccttc acgagctgct ggaccccgcg atggaacggc
     8821 ccggtgtcga gcagccacca gtcgagcgcg tggtcgtggt tccactcgtc ggactgcccg
     8881 aactccccgc ccatgaacag gagcttcttg cccgggtagg cccacatgaa cgcgtagagc
     8941 aggcgcaggt tggcgaagcg ctgccagtcg tcgcccggca tcttgtgggc gagcgagcgc
     9001 ttgccgtaga ccacctcgtc gtgggagagc ggcaacacga agttctcggt ccacgcgtag
     9061 agcagcccga aggtgagctg gttgtggtgg tacttccggt ggaccggctg gtgctgcatg
     9121 tactcgagga tgtcgtgcat ccaccccatg ttccacttga agccgaagcc gaggccgccc
     9181 aggtagaccg ggcgcgacac ctgcggccac gcggtggact cctccgcgat ggtggaggcg
     9241 cccggctggc ccccgtatac cacctcgttc agcttcttga ggaaggcgag ggcctcgagg
     9301 ttctcccggc cgccgaactc gttgggtatc cactcgcccg ccttgcggga gtagtcgcgg
     9361 tagatcatgg aggcgaccgc gtcgacccgc aggccgtcga tgtggtagcg ctcgagccag
     9421 aacaccgcgt tggcgatgag gaagttggag acctcgcgcc gcccgaagtt gaacacccgg
     9481 gtgccccagt ccggatggtc gcgcaggttc gggtcgtcgt gctcgtacag gtgagtgccg
     9541 tcgaagaagg cgagcccgtg cgcgtccttg ggaaagtggg cgggcaccca gtccatgatg
     9601 accccgatgc cccgccggtg gagggtgtcc acgaaggcca tgaagtcggt gggcgtgccg
     9661 tagcggcggg tgggcgcgta gtagccgagg gtctggtagc cccaggaggc gtagtagggg
     9721 tgctcggtca ccgggagcag ctccacgtgg gtgaagccca gctcggtcac gtagtcggcg
     9781 agttgctcgg ccacctcgcg gtagctcggc agggtgcccc cctcggggcg ccgccatgag
     9841 cccaggtgca cctcgtacac cgacatcggc cggtgcaggg cgttccggcg cttgcgctcg
     9901 tccatccact ccgcgtcgcc ccacgtgtag gtgttgaggt cggtgaccgc ggaggcggtg
     9961 cgcggctcgt cgggctcgaa ggcgaacgcc accgggtcgg tcttgagcgc gagcagcccg
    10021 ccgtcccgtc cgatgatctc gtacttgtag cgcgcgccga gggtcacccc ggggacgaac
    10081 agctcccaca gcccgttgcc ggggtgcagg cgcatcggct gacgccgccc gtcccagccg
    10141 ttccagtcgc cgaccacgct gacccgccgc gcgttgggcg cccacacaac gaagcgcacg
    10201 ccctccacgc cctcgtgacg gatgggatgc gcgccgagcc gctcgaagag gcggtagtgg
    10261 gtgccctcgc cgagcaggtg caggtcgtag gcgctgaggg tcggtgggaa gcggtagggg
    10321 tcctcgacct cggagacctg cccctgctcg tcgaccactc gcagccggta cgcgaggggc
    10381 aggcggcggc ccggcacctc gccttcccag aagccggccg gatgcaccgc gcgcagcggg
    10441 ctgacggtgc cgtcgggcat caccgccgcc accgcgcggg cggtggggac cagggcccgc
    10501 acgctgacca tgccctc
//
LOCUS       DATMCV010000396         3539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_683747, whole genome shotgun sequence.
ACCESSION   DATMCV010000396 DATMCV010000000
VERSION     DATMCV010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683747"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VNU03_09200"
     CDS             <1..506
                     /locus_tag="VNU03_09200"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HWP74681.1"
                     /translation="AGRGFVERLMKRPVPASYGQTTYYAEHAFRFTSADNTSRFGRYR
                     FVPEAGEAFLSPDEAGKRSASFLSNELDGRLRTGPVAFRLLLQVAESGDPTDDVTALW
                     PEGRPLVELGRLEITGISPTSAGDERRLIFDPTNRTDGIDLSADPILLARSAAYSISY
                     ERRSKGE"
     gene            complement(538..2139)
                     /locus_tag="VNU03_09205"
     CDS             complement(538..2139)
                     /locus_tag="VNU03_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74682.1"
                     /translation="MRGRLIVLILAILAVLVAGVSAGAQESPRRGGELIFLVPAEPPS
                     YDAHREETFAVVHPAAPHYSTLLRVDPTDRTGTRIVGDLAESWTVGADGRTYTVRIRR
                     GVRFHDGSELTAHDVKASYDKIIFPPSGVTSNRKGEYIAVETVTAPDPYTVVFRLKWP
                     SASFLSSLASPFNWIYKADILARDIHWYEKNVMGTGPFLFVEYVKGSHWTGRRNPDYW
                     DKGKPYLDGYRALFVRDSAAQIAAIRGQRAMIQFRGFSPPERDTLVQALGNAVTVQES
                     PWECSNPLAINHERKPFDDPRVRRALSLALDRHQGATVLSRLTILREVAGVLGVGTPL
                     ATPPADLEKLAGYSRDIAKSRAEARRLLREAGVPEGFAFTFKNRGIPNPYDALGVWLV
                     DQWRQIGLNVRIETLELGMLYRAVFSGDFEVAADFHCSYIVEPDLALFKFQSMDVSPI
                     NYSRYVDRTLDELYQKQNRAADREARRRYIRDFERRLLDEEAHYIYTFQWHRIVVHSA
                     RVRGWTITPSHFLNNQLDTVWLAEP"
     gene            complement(2141..3190)
                     /locus_tag="VNU03_09210"
     CDS             complement(2141..3190)
                     /locus_tag="VNU03_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWP74683.1"
                     /translation="MMKAVVLHEADEMRVEQRPVPDPGSGEVLLKVNVTSICGTDVKV
                     LHRKLLGQPDGPFIMGHEYAGTVAALGPGVDEFKVGDRVAVEVHKGCERCENCIKGWY
                     TSCLNYGNLAKGHRAKGLTCDGGFAEYAVNHVNTLYKLPDNLTFEQACMVTTAASPLW
                     AIDLMGGYLAGETVLVQGPGPIGLLAVQLCKALGAERVILSGTRPERLEIGRRLGANF
                     LVNVRQESLARRVADITDGKGADSVLECAGGPTSMQEALESVKRGGRIGVVAWYAAPV
                     EMDMNLAVRSNVRIYAARGEGGMNSGRSLALMGAGKLDADAIITHRFALDEVHEAFHT
                     YVDRIGNALKVVMRV"
     gene            complement(3297..>3539)
                     /locus_tag="VNU03_09215"
     CDS             complement(3297..>3539)
                     /locus_tag="VNU03_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HWP74684.1"
                     /translation="EGQAAPNITPDNATGIGEWTEEQIVTYLGTGNRPDGDVAGGLMG
                     ENIQGTLAGYKDMTQADLRAIAQFLKSIPAVKNRIE"
BASE COUNT          540 a         1214 c         1217 g          568 t
ORIGIN      
        1 cggccgggcg cggcttcgtc gagcgcctga tgaagcggcc ggtaccggcc agctacgggc
       61 agacaaccta ctatgccgag cacgccttcc gcttcacgtc cgcggataac acgagccggt
      121 tcggtcgcta tcgcttcgtg ccggaggccg gcgaggcctt tctctcccct gacgaggccg
      181 gcaagcgaag cgcgagcttc ctgagcaacg agctggacgg ccgcttgcgg accggaccgg
      241 tcgcgttccg gcttctcctg caagtagccg agtccggcga cccgacggac gacgtcaccg
      301 cgctctggcc cgaggggcgg cccctggtcg agctggggcg tctggagatc accggcatct
      361 ctccgacgag cgcgggcgac gagcggcgcc tgatcttcga cccgaccaac cggacggacg
      421 gcatcgatct ctcggcggat ccgatcctgc tggcgcggtc ggccgcctat tccatctcct
      481 acgagcggcg cagcaagggc gagtgagcca gcatccgtcc gtctagctcg acgagcgcta
      541 gggctcggcc agccacacgg tgtcgagctg gttgttcagg aaatggctcg gggtgatcgt
      601 ccagccccgg acccgcgcgc tgtggacgac gatccggtgc cactggaacg tgtagatgta
      661 gtgcgcctcc tcgtcgagca ggcgccgctc gaagtcgcgg atgtaacgcc ggcgcgcctc
      721 gcgatcggcg gcccggttct gcttctggta gagctcgtcg agcgtgcggt ccacgtagcg
      781 gctgtagttg atggggctca cgtccatcga ctggaacttg aacagggcca ggtccggctc
      841 gacgatgtag ctgcagtgga agtcggccgc gacctcgaag tcgccgctga agacggcgcg
      901 gtagagcatg cccagctcga gcgtctcgat ccgcacgttg aggccgatct gccgccactg
      961 gtcgacgagc cacaccccga gcgcgtcgta gggattcggg atcccccggt tcttgaaggt
     1021 gaaggcgaag ccctcgggga cccccgcctc gcgcaggagg cgacgggcct ccgcccgcga
     1081 cttcgcgatg tcgcgcgagt agccggcgag cttctccaga tctgcgggcg gcgtggccag
     1141 gggggtcccc acgcccagca cgcccgccac ctcgcggagg atggtgagcc gcgacagaac
     1201 cgtcgcgccc tggtgacgat ccagagcgag cgagagcgcg cgccgcacgc gcgggtcgtc
     1261 gaacggcttg cgctcgtggt tgatcgcgag cggattgctg cactcccagg ggctctcctg
     1321 cacggtgacc gcgttgccca gggcctgcac gagggtgtca cgctccgggg gcgagaagcc
     1381 tcggaactgg atcatcgcgc gctggccgcg gatggccgcg atctgcgcgg ccgagtcccg
     1441 cacgaatagc gcccgatacc cgtcgaggta cggtttgccc ttgtcccagt agtcggggtt
     1501 ccgccggccg gtccagtggg agcccttcac gtactcgacg aagaggaacg gcccggtccc
     1561 catgacgttc ttctcgtacc agtggatgtc ccgggccagg atgtccgcct tgtagatcca
     1621 gttgaagggg gaagcgagcg aggacagaaa cgaggcggaa ggccacttca ggcgaaagac
     1681 cacggtgtac ggatcgggag ccgtgaccgt ctcgaccgcg atgtactcgc ccttgcgatt
     1741 cgaggtgacc ccggacgggg gaaagatgat cttgtcgtag ctcgccttga cgtcgtgcgc
     1801 ggtcagctcg ctgccgtcgt gaaagcggac gccgcgccgg atccgaaccg tgtaggtccg
     1861 gccgtccgcg ccgaccgtcc acgactcggc gaggtcgccg acgatccgcg tgcccgtgcg
     1921 atcggtgggg tccacccgca gcagcgtgct gtagtgcggc gcggcggggt gcaccaccgc
     1981 gaaggtctcc tcgcggtggg cgtcgtacga cggaggctcg gcgggcacca ggaagatcag
     2041 ctcgccgccg cgccgcggac tctcctgggc gccggccgag acgcccgcca ccaggaccgc
     2101 caggatcgcc aggatcagga cgatcagccg cccgcgcatg ctacacgcgc atgaccacct
     2161 tgagcgcgtt gccgatccgg tcgacgtagg tgtggaaggc ctcgtgcact tcgtctagcg
     2221 cgaagcgatg ggtgatgatg gcgtccgcgt cgagcttgcc ggcgcccatc agggccaggg
     2281 agcggcccga gttcatcccg ccttcccccc gggccgcata gatccgcacg ttgctgcgga
     2341 cggccaggtt catgtccatt tcgacgggag cggcgtacca cgcgacgacc ccgatgcggc
     2401 cgccgcgctt gacgctctcg agcgcttcct gcatcgacgt ggggccgccc gcgcactcga
     2461 ggacggaatc cgcccccttg ccgtcggtga tgtcggccac gcgccgggcg aggctctcct
     2521 ggcggacgtt gacgaggaag ttggccccga gtcgccggcc gatctccagg cgctcgggac
     2581 gcgtgccgga gagaatcacc cgctcggccc cgagcgcctt gcacagctgg acggcgagca
     2641 ggccgatggg gcccgggccc tggacgagca cggtctcgcc ggcgagatag ccccccatga
     2701 gatcgatcgc ccagagggga gaggccgccg tggtcaccat gcaggcctgc tcgaacgtga
     2761 ggttgtcggg gagcttgtag agcgtgttga cgtgattcac cgcgtactcg gcgaacccgc
     2821 cgtcgcacgt cagccccttg gcgcgatggc ccttggcgag gttgccgtag ttgagacacg
     2881 acgtgtacca gcccttgatg cagttctcgc agcgctcgca gcccttgtgg acctcgacgg
     2941 ccacgcgatc gcccaccttg aactcgtcca ctcccggccc cagcgcggcc accgtccccg
     3001 cgtactcgtg ccccatgatg aagggcccgt cgggctggcc gaggagcttg cggtgcagga
     3061 ccttcacgtc ggtcccgcag atggaggtga cgttgacctt gagcaggacc tcccccgatc
     3121 ccggatcggg caccggccgc tgctccacgc gcatctcgtc ggcctcgtgc aggaccaccg
     3181 ctttcatcat cgcgccacca cctcccgcga gacgggagtc tacgtggacg aagagaccgc
     3241 tgtcaaaggc ctctcagtag cagcagcccc agtggggcgg cacccagacg ggggcgctac
     3301 tcgatccggt tcttcaccgc gggaatgctc ttgagaaact gcgcgatcgc gcgcagatcg
     3361 gcctgggtca tgtccttgta gccggccagc gtcccctgga tgttctcgcc catgagcccg
     3421 ccggccacgt cgccatcggg tcgattgccc gttccgaggt acgtgacgat ctgctcctcg
     3481 gtccactcgc cgatcccggt cgcgttgtcg ggggtgatgt tcggcgccgc ctgaccctc
//
LOCUS       DATMCV010000397         3314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_756311, whole genome shotgun sequence.
ACCESSION   DATMCV010000397 DATMCV010000000
VERSION     DATMCV010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756311"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VNU03_09220"
     CDS             complement(<1..216)
                     /locus_tag="VNU03_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWP74685.1"
                     /translation="MLLNLAKAGGPLYRRVYLALKTKIRAGGLGPATRVPSTRALARD
                     LGVSRNTVMLAYEQLAAEGYLVSRHRGA"
     gene            340..705
                     /locus_tag="VNU03_09225"
     CDS             340..705
                     /locus_tag="VNU03_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWP74686.1"
                     /translation="MASRTDDLETLEALNQGYLLAAEKSDVAWYGEHLAEDYRATNPD
                     GSFVDKAGFLARIGRPHTFTNFRAPEVRIQRVGDLALIHASFEDTRPDGGHGRGRYTD
                     IWQRRDGRWLCVSAHFTRC"
     gene            708..1064
                     /locus_tag="VNU03_09230"
     CDS             708..1064
                     /locus_tag="VNU03_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWP74687.1"
                     /translation="MATDQATLEALNREYIRSVSEADVRWFDANLSADFVNSNPDCSL
                     LDRAGFLAQIARGSTVKNLDIEDVMIRVLGDGALIHARTTYTKADGQPGAGRYTDVWA
                     RRGAGWVCVAANVTRL"
     gene            complement(1071..1550)
                     /locus_tag="VNU03_09235"
     CDS             complement(1071..1550)
                     /locus_tag="VNU03_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP74688.1"
                     /translation="MGPKGEEFARQFEAKVEQATALLEGLTDADWKKTTAAEKWTVAV
                     TAHHIASSYESATRIIETIAAGRTLPHFTRAMLDEMNAQHARQFAGCTRAETIALHKK
                     GAAAAAAVVRGLSDSELARAGTVFARMPPVSAEELVQRILLAHIDAHFGSIRKAIAG"
     gene            1755..2042
                     /locus_tag="VNU03_09240"
     CDS             1755..2042
                     /locus_tag="VNU03_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74689.1"
                     /translation="MRKSQRIAAAVTVGVLLWIGSALGQGKPTCTSQGKVQTAQKVVG
                     QVVKVDAGLDKLTVREDNGTVHEFQASKDALREFKAGDHIEAMLREAPKCQ"
     gene            2103..>3314
                     /locus_tag="VNU03_09245"
     CDS             2103..>3314
                     /locus_tag="VNU03_09245"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWP74690.1"
                     /translation="MPDVSVWRRLGMASAVGLWALASVSSLGAQVPREEPRLALLIGN
                     SAYRESPLRNPVNDVRAMAQRLRELGFTVLVHENATKRTMEAAIIEFGRRLAEGGVGA
                     FYYAGHGLQVRGRNYLVPVDAEIEDEASTRVAAVDVELLLEQMAEAKNRVNMVILDAC
                     RNNPFERRMRGGSRGLAAVDAARGTLVAYATAPGSVAADGDGKNGLYTEELLEALREP
                     GLKIEEVFKRVRINVARRSKGAQTPWESSSLTGDLVVNVTVNVTAAAVSPAQAPDREG
                     LFWMSIKDSTDPAAFEAYLKQYPQGTFAPLARLRLASVSQPARPRDLGRFDGAWNVTV
                     ECPAHGGASAYTRRLLAQVTDGTLAAQLGNVGQAGSLTLSGKIQPDGKASLDARGMVG
                     DPRNTANRLSQG"
BASE COUNT          526 a         1135 c         1151 g          502 t
ORIGIN      
        1 cgcgccccgg tggcggctca ccaggtagcc ctccgccgcg agctgctcgt aggccagcat
       61 cacggtgttg cgcgacacgc cgaggtccct cgccagcgca cgcgtggagg gaaccctggt
      121 ggccggtccg aggcccccgg cccggatctt ggtcttgagc gcgaggtaca cgcggcgata
      181 gagcggcccg cccgcctttg ccaggttcag cagcatggcg cctcgctcgg ggcctggccg
      241 atctggcccc tttgattcgt ctgaatctgg ctcttcccag gtgccagcat gggagctagg
      301 atagccctgc tcgaacgaac ccggaaggag gaaatcacca tggcgagcag gacggacgac
      361 ctcgagaccc tcgaggcctt gaaccaggga tatctcctcg cggcggagaa gtccgacgtg
      421 gcctggtacg gagagcacct ggccgaggac taccgcgcca ccaaccccga cggctccttc
      481 gtggacaagg cgggcttcct ggcccgtatc ggacggcccc acacgttcac gaacttccgc
      541 gcccccgagg tccgcattca gcgggtgggc gacctcgccc tgatccacgc cagcttcgag
      601 gacacgcggc cggacggcgg ccacggccgc ggacgctata ccgacatctg gcagcggcgg
      661 gacgggcgct ggctctgcgt gtccgcccac ttcacgaggt gctgaccatg gcgaccgacc
      721 aggcgacgct cgaggcgctc aaccgtgagt acatccgatc ggtcagcgag gcggacgtgc
      781 ggtggttcga cgcgaatctc tcggccgact tcgtcaacag caatcccgac tgctcgctcc
      841 tcgaccgggc gggcttcctc gcccagatcg cgcgcggatc gacggtgaag aacctcgata
      901 tcgaggacgt catgatccgc gtgctgggtg acggggcgct gatccacgcc cgcaccacgt
      961 acaccaaggc ggacggtcag ccgggcgccg ggcgctacac cgacgtgtgg gcgcgacgcg
     1021 gggccggctg ggtgtgcgtg gcggccaacg tcacgcgtct ctgagtcgac tcagcccgcg
     1081 atcgccttcc ggatgctgcc gaaatgcgcg tcgatgtggg cgaggaggat gcgttgcacc
     1141 agctcctcgg cgctcaccgg tggcatcctc gcgaacacgg taccggctcg ggccagctcg
     1201 ctatccgaga ggcctcgcac gactgcggcc gccgccgcgg cccccttctt gtggagcgcg
     1261 atcgtctccg cccgtgtgca ccccgcgaac tgccgggcat gctgggcgtt catctcgtcg
     1321 agcatcgcgc gggtgaagtg cggcagcgtc cggccggcgg cgatcgtctc gatgatccgg
     1381 gtagccgact cgtagctgct ggcaatgtgg tgcgccgtca ccgcgaccgt ccatttctcg
     1441 gccgcggtcg tcttcttcca gtcggcgtcg gtcaaccctt ccagaagggc cgtcgcctgt
     1501 tccactttgg cctcgaactg cctggcgaat tcttctccct tgggtcccat gaatacctcc
     1561 ccggagtctg cctccactag cacgacgaac gaggcggggt gaaatcgacg tcggccgtcc
     1621 cggaaccttc ctgcgctcac gaccgccgcc ctgctctata ctggggccca tctcgcatcc
     1681 gggcgccctc atcggcaccg gcgttgggcc cggcgacgtc atccacgact tcacgaagag
     1741 acagggaggg gacgatgcgc aagagccagc ggatcgcggc agccgtgacg gtgggagtac
     1801 tgctctggat tggaagcgct ctggggcagg gcaagccgac ctgtacgtcc caggggaagg
     1861 tccagacggc gcagaaggtc gtggggcagg tcgtgaaggt cgatgcggga ctcgataagc
     1921 tgacggtgcg agaggacaac ggaacggtcc acgagttcca ggcctccaag gacgcgcttc
     1981 gggagttcaa ggccggcgat cacatcgagg cgatgctccg cgaggccccc aagtgccagt
     2041 agcccaagtg ccaatagatt gacgaggcgg cctggctcct ctttcccgcc ggggaggacc
     2101 ggatgccgga tgtgagcgtg tggcggcgcc tcgggatggc ctccgcggtc ggcctgtggg
     2161 ctctcgcctc ggtgtcgtct ctcggagccc aggtgccccg cgaggagccc cggctcgccc
     2221 tgctgatcgg caacagcgcc taccgcgagt cgcccctgcg caacccggtg aacgacgtgc
     2281 gggcgatggc gcagcggctc agggagctcg gcttcaccgt cctcgtccac gagaacgcca
     2341 ccaagcgcac gatggaggcg gcgatcatcg agttcgggcg tcgcctcgcc gagggcggcg
     2401 tcggcgcctt ctactacgcg ggccacgggc tgcaggtccg tgggcgcaac tacctggtgc
     2461 cggtcgacgc cgagatcgag gacgaggcat ccacccgcgt cgccgccgtc gacgtggagc
     2521 tgctgctcga gcagatggcc gaggcgaaga atcgcgtcaa catggtgatc ctcgatgcct
     2581 gccgcaacaa cccgttcgag cgccggatgc gtggtgggtc ccggggtctg gcggcggtcg
     2641 acgcggcccg ggggacgctg gtggcctacg ccaccgcgcc gggatcggtg gccgcggacg
     2701 gcgacggcaa gaacggtctg tacaccgagg agctgctgga ggcgctgcgc gagcctggcc
     2761 tcaagatcga ggaggtattc aagcgcgtac ggatcaacgt cgcgcgccga tcgaagggcg
     2821 cccagacccc atgggaatcg tcctcgctca ccggagacct cgtggtgaac gtgaccgtca
     2881 acgtcacggc ggcggccgta tcgccggcgc aggcgcccga tcgcgagggc ctgttctgga
     2941 tgtcgatcaa ggacagcacc gatcccgcgg ccttcgaggc ctatctcaag cagtatcccc
     3001 aggggacgtt cgcccccctc gcccgcctgc ggctggccag cgtgagccag ccggcccggc
     3061 cgcgggatct cgggcgcttc gatggcgcct ggaacgtcac cgtcgaatgc ccggcacacg
     3121 ggggcgcctc cgcctatacg agacggctcc tcgcgcaggt gacggacggg accctggcgg
     3181 cgcagctcgg aaacgtgggg caggcgggct cgctgacgct cagcgggaag atccagccgg
     3241 acggcaaggc cagcctcgac gcccgcggca tggtgggcga tccgaggaac accgccaacc
     3301 gcttgagcca gggc
//
LOCUS       DATMCV010000398         3004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_90224, whole genome shotgun sequence.
ACCESSION   DATMCV010000398 DATMCV010000000
VERSION     DATMCV010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90224"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VNU03_09250"
     CDS             <1..272
                     /locus_tag="VNU03_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP74691.1"
                     /translation="LFGAASGPVGPLDLQRLNAGGSLFVTRPSLNHHIATRDELVTRS
                     TELLGWIRDGKVKLRVEHQFPLEQAAEAHRQLEGRKTTGKILLIP"
     gene            337..1944
                     /locus_tag="VNU03_09255"
     CDS             337..1944
                     /locus_tag="VNU03_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HWP74692.1"
                     /translation="MIKWLLIGLIAVGVLVVGALLAVPLLLDSPAMQTYVQQQASHAL
                     GRPVKFGGLSISALPLPSVRLRQLQVAEDPAFGPGPFVTVNEGRMRIRLRPLLQGRVE
                     LADLTLDEPRIHVIEDAAGRLNLATLGPSGPPGTSPPPRVGGGRPGPAGAGAVLLSRV
                     RITNGAVEYEKIEGPGKKSGALALDRINVTVTQGAVGEPLRVTGDARGPGDVKLTIAE
                     GSVSLGPGRAVGDAQVKAMVDVEGRDVRAVVGTLVPAPAVSGPIKGRVQVSGTAARLT
                     ATGAVTFDRLTLTEAPPHCPDPKPRTLVLDEVRVPLLYTPPQLESQQVQTKVAKGTVT
                     FRLLVALGPPRVVTLRDIQVKGVQLEPVLVDYLCQRNAVTGPLDLTGEMSLRLPESMP
                     TLAGSGRLSIGAGRVVGPDLLKAAGQALALTDLVGAALTPGGRGGSGAKSPLNFDSIT
                     ATYTITNGVIRTDDLVYTARDVRVTGAGTYGLVDGRTAMDVTVNQGTNRVKARVAGGP
                     GALSIVPTDVRLKEPKDLKKALDRLLR"
     gene            complement(1913..2761)
                     /locus_tag="VNU03_09260"
     CDS             complement(1913..2761)
                     /locus_tag="VNU03_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP74693.1"
                     /translation="MREPAPDVVDFYDRHPINETQVLAAARDGGAGLRALRPEDLWRW
                     DQDHYGGLAAVEALAQRAAIAPGMAVLDVCAGLGGPARFLAYRFGARVTGVDLTHSRC
                     AAGARLTALVQLGPLVRHVRGDAQVLPFRSRAFDAAVSQEGLLHVPDKPAVLAECARV
                     LRPGARLAFSDWIARPRLEDNERRRLFAWMAAASLQSIDGYRGLLTRAGFDGIVAEDL
                     SGEWIAILQQRLRMYRALRKQTVARLGQARYDEYNQLYGFFVALVEAGKLGGARLSAA
                     AGRAPS"
     gene            2856..>3004
                     /locus_tag="VNU03_09265"
     CDS             2856..>3004
                     /locus_tag="VNU03_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006910478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWP74694.1"
                     /translation="MTDAAVNPLKAGLEDVVVSPSEICFIDGRKGRLIYRGYDVDDLV
                     AHSTF"
BASE COUNT          429 a         1071 c         1116 g          388 t
ORIGIN      
        1 cgctcttcgg ggcggcgagc gggccggtgg ggccgctcga tctccagcgg ctgaacgcgg
       61 gcggctcgct cttcgtgacg cggccgagcc tgaaccatca catcgccacc cgcgacgagc
      121 tggtgacgcg ctccaccgag ctgctcggct ggatccgcga cggcaaggtc aagctgcgcg
      181 tcgagcacca gttccccctc gagcaggccg ccgaggcgca ccggcagctc gagggacgca
      241 agaccaccgg caagattctc ctgattccat gattcgcgat cggcgcacgg ggcggcccgc
      301 cgtcgccccg tgcgctgacg gcacgacgct cccgccatga tcaagtggct gctgatcggc
      361 ctgatcgccg tcggggtgct ggtggtggga gcgctgctcg cggtgcctct gctgctcgac
      421 agtccggcca tgcagaccta cgtgcagcag caggcgagcc acgccctggg gcggccggtg
      481 aagttcggcg ggctctcgat ctccgccctg ccgctcccga gcgttcggct ccgccagctc
      541 caggttgccg aggacccggc gttcgggccg ggcccgttcg tgacggtcaa cgaaggtcgc
      601 atgcgcatcc ggctccggcc cctgctgcag ggccgcgtgg agctggccga tctcacgctc
      661 gacgagcccc ggatccacgt gatcgaggac gcggccggcc ggctcaacct ggccacgctg
      721 ggcccctcgg gcccgcccgg cacgagcccg ccgcctcgcg tgggcggggg gcggcccggg
      781 cctgcaggag cgggggcggt gctgctctcc cgggtgcgca tcaccaacgg cgcggtcgag
      841 tacgagaaga tcgaggggcc cggcaagaag agcggcgcgc tcgcgctcga tcgcatcaac
      901 gtcacggtga cccagggggc ggtgggcgag cccctgcgcg tgaccggcga cgcgcggggc
      961 ccgggcgacg tcaagctgac gatcgcggag ggcagcgtga gcctcgggcc cggccgggcg
     1021 gtcggcgacg cgcaggtgaa ggcgatggtc gacgtggagg gccgcgacgt gcgcgcggtg
     1081 gtgggcacgc tcgtgccggc gccggcggtg tcgggcccga tcaaggggcg cgtccaggtc
     1141 tcggggacgg cggcgcgact gaccgccacc ggcgccgtca ccttcgaccg cctcaccctg
     1201 acggaggcgc cgccgcactg ccccgacccg aagccgcgga cgctcgtcct cgacgaggtg
     1261 cgggtgccgc tgctctacac gccgccgcag ctcgagagcc agcaggtgca gacgaaggtg
     1321 gcgaagggga cggtcacctt ccggctgctg gtggcactcg gcccgccgcg cgtggtcacc
     1381 ctgcgggaca tccaggtgaa gggcgtgcag ctcgagccgg tcctggtcga ctacctctgc
     1441 cagcgcaacg cggtgacggg gccgctcgac ctgaccggtg agatgagcct gcgactgccg
     1501 gagtcgatgc cgactctcgc cggctccggc cggctctcga tcggggccgg gcgcgtggtc
     1561 ggtcccgatc tgctcaaggc ggcaggccag gccctcgcgc tgaccgacct cgtgggggcc
     1621 gcgctcaccc cggggggacg cggcgggtcc ggggcgaagt caccgctcaa cttcgactcg
     1681 atcaccgcca cctacaccat caccaacggc gtgatccgca ccgacgacct ggtctacacg
     1741 gcgcgcgacg tgcgcgtgac cggagccggc acctacggcc tggtggacgg ccgcaccgcg
     1801 atggacgtca cggtgaacca gggcacgaat cgggtgaagg cgcgggtggc gggcggcccg
     1861 ggcgcgctct ccatcgtgcc caccgacgtg cggctcaagg agccgaagga cctcaagaag
     1921 gcgctcgacc ggctgctgcg ctgaggcgcg cgccgccgag cttgcccgcc tccacgagcg
     1981 ccacgaagaa gccgtagagc tggttgtact cgtcgtagcg cgcctggccg agccgggcca
     2041 ccgtctgctt ccgcagggcc cggtacatcc gcaggcgctg ttgcaagatt gcgatccact
     2101 cgccggagag gtcctcggcc acgatgccgt cgaagccggc gcgggtcagc aggccgcggt
     2161 agccgtcgat gctctggaga ctcgccgcgg ccatccacgc gaagagccgc cggcgctcgt
     2221 tgtcctcgag tcgcggccgc gcgatccagt cgctgaaggc gagccgggcc ccgggcctca
     2281 gcacgcgcgc gcactccgcg agcaccgccg gtttgtccgg cacgtgcagc agaccctcct
     2341 ggctgaccgc agcgtcgaag gcgcgggagc ggaagggcag aacctgggca tcgccgcgca
     2401 cgtgccggac cagggggccg agctggacca gcgcggtgag gcgcgccccc gcggcgcagc
     2461 gcgagtgggt gaggtcgacg ccggtcaccc gggccccgaa gcgataggcg aggaagcgcg
     2521 cggggccgcc gagcccggcg cagacgtcga gcaccgccat cccgggagcg atggcggcgc
     2581 gctgcgccag cgcctccacc gccgcgaggc cgccgtagtg atcctggtcc catcgccaga
     2641 gatcctccgg ccggagcgcg cgcaggccgg cgccgccgtc gcgcgcggcg gccagcactt
     2701 gcgtctcgtt gatcgggtgg cgatcgtaga aatccaccac gtcgggagcg ggctcgcgca
     2761 cgacgggcag tgtaggggag ggctgccttg aagtcacgga gggcgtatgg tagaaggagt
     2821 cgtttccatc cgccaccaag gagagccgga acatcatgac ggacgccgcg gtcaatcccc
     2881 tgaaggccgg tctcgaggac gtcgtcgtct ccccgtctga gatctgtttc atcgacggcc
     2941 gcaagggccg cctgatctac cgaggctacg acgtcgacga tctggtggcg cactcgacct
     3001 tcga
//
LOCUS       DATMCV010000399         4180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_19740, whole genome shotgun sequence.
ACCESSION   DATMCV010000399 DATMCV010000000
VERSION     DATMCV010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19740"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VNU03_09270"
     CDS             complement(<1..271)
                     /locus_tag="VNU03_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWP74695.1"
                     /translation="MAYMEGRSGVLPAVIVGLCLVIGLSVAGYFIGRGATRFRSDSRV
                     VTVKGLVEREVKADQAVWTLSLRRASETLPDAHARISADRDAAVAF"
     gene            complement(294..1466)
                     /locus_tag="VNU03_09275"
     CDS             complement(294..1466)
                     /locus_tag="VNU03_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HWP74696.1"
                     /translation="MSLVRDLSVSAVVAGFVTVLVGFASAAVIVFQAAGAVGATEAQV
                     SSWLLALGLGMGLTCSLLSLRYRVPIVTAWSTPGAALLATSGAGLSIEEATGAFLVSA
                     ALVTLVGFSGWFERAMDRIPVPIASGMLAGVLLRFGLGLFTAMSTQLTLALAMFLAYL
                     AARRLAPRYAVIVALLVGVAIALVQGLLHVDAVRLALARPVWVTPRFTPGAVVGVALP
                     LFVVTMASQNVPGVAVIRASGYTVPISPLIGWTGLANLALAPFGAFTVNLAAITAAIC
                     MGREAHEDPRRRYVAAVFAGIFYLIVGIFGATVVALFLAFPAELVAAIAGLALLATIG
                     NGLASAVGSENQREPALVTFLVTASGIAPAGIGSAFWGLVAGVMTLAILRAAGRRA"
     gene            1537..2460
                     /locus_tag="VNU03_09280"
     CDS             1537..2460
                     /locus_tag="VNU03_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74697.1"
                     /translation="MRAYLLSRLGQTLLVVFLSLTAVFFLVRLGGDPVLLFLPMDIQA
                     KDLNEFRQRLGFNDPLGVQYGRFMGRALHGDFGESLRYKNDALQLVLGRLPATLWLAL
                     AALLLTFCVAVPVGVFSAVRRGSLFDLFGMTGAVLGQAVPGFWLGLMMIYLFSVRLGW
                     LPTGGMGGPLHFVMPCVVLASFYAARMARLTRSSVLDTLNEEFVLTARAKGLAERIVI
                     GKHTLKNSAIPLVTLAGLDTGQLLGGAVITETIFAWPGLGRLTVQALLNRDFPVVLAA
                     VFVFSVTYTLINFAVDLLYGWLDPRTRRQRA"
     gene            2457..3281
                     /locus_tag="VNU03_09285"
     CDS             2457..3281
                     /locus_tag="VNU03_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74698.1"
                     /translation="MRVFVARHGRGVLGLLIATLVLLAAVAAPVLVAQDPVQTDFAAG
                     LKPPGTPGHPLGTDQLGRDLLARVLYGARVALFIGLCCVLLTALVGGLLGLLAGFYEG
                     WPGAVLMRIADVQLSFPFILLALTINAIVGLGLRNIIVSLSVAGWVVYARVVRGEVLS
                     VKQRDFVHAAQALGVGRARVLFRHVLPNVAPSIVIVASLQFAQFIVAEAAISFLGFGV
                     QPPTPAWGSMLSESRDYLYVAWWLAAFPGAALAFTALGINLVGDWLRDVLDPRFRV"
     gene            3281..3715
                     /locus_tag="VNU03_09290"
     CDS             3281..3715
                     /locus_tag="VNU03_09290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP74699.1"
                     /translation="MAVVIRPCRPDEGPALLDLWHHADTSPSPTDTPEQVDQVIQDPA
                     ASVLVAVDRDLLVGSIIGGWDGWRGNIYRLAVLPSYRRRGIARALVAAAEERLAARGA
                     RRVSALVEHDHPWAVGFWDSLYEPDPRMIRYVRKVDSTRRPS"
     gene            3712..>4180
                     /locus_tag="VNU03_09295"
     CDS             3712..>4180
                     /locus_tag="VNU03_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74700.1"
                     /translation="MTRFTRMLAALSLLLGVAVAVPLADPAGAQDRAKTKEIVVAFAA
                     EPRTLLPDTIVDWTTNNMVEHMYDRLVDRDPKSYKPSPMLATAWKVVNDTTWEFTLRQ
                     GVKFHNGEPFNAQSVKATMEYIKDPANKTHYLPRWSQVKEVQIVNDYTVRFVTE"
BASE COUNT          605 a         1546 c         1423 g          606 t
ORIGIN      
        1 ggaacgcgac cgcggcatcc cggtcggcgc tgatccgcgc gtgggcgtcc gggagcgtct
       61 cgctcgcccg gcggaggctg agcgtccaca ccgcctggtc cgccttcacc tcgcgctcga
      121 ccagtccctt gaccgtgacc accctcgagt ccgaccggaa ccgcgtggcg ccccggccga
      181 tgaagtaacc cgcgaccgac agcccgatca ccaggcagag gcccacgatg accgcgggga
      241 gcacccccga ccgcccttcc atgtacgcca tgcccgctcc ttccggggcc gcgctaggcg
      301 cgacggcccg ccgcccgcag tatcgccagg gtcatcactc ccgccaccag tccccagaac
      361 gccgatccga tgccggccgg cgcgatgccg gacgcggtca ccagaaacgt gacgagcgcc
      421 ggctcccgct gattctcgct gccgaccgcg gaggcgaggc cattgccgat cgtggccagc
      481 agggccagcc ccgcgatcgc cgccaccagc tcggccggga acgcgaggaa gagggccacc
      541 acggtggccc cgaagatccc gacgatcaga tagaagatcc cggcgaagac cgccgccacg
      601 tagcgccggc gcggatcctc gtgcgcctcg cggcccatgc agatggccgc ggtgatcgcg
      661 gccaggttca cggtgaacgc gccgaagggc gccagcgcca ggttggccag cccggtccag
      721 ccgatgagcg gcgagatcgg caccgtgtag ccggaggccc ggatgaccgc gacccccggc
      781 acgttctggg acgccatggt gaccacgaac aggggcagcg ccaccccgac caccgcgccc
      841 ggggtgaagc ggggcgtgac ccagaccggc cgggccagcg cgaggcggac cgcatccacg
      901 tgcaggagac cctggacgag cgcgatcgcg accccgacca gcagcgccac gatcaccgcg
      961 taccggggcg cgagccgccg ggccgcgaga tacgcgagga acatggcgag ggccagggtg
     1021 agctgcgtgc tcatcgcggt gaagagaccg aggccgaagc gcagcagcac gccggccagc
     1081 atgccggagg cgatcggcac cgggatgcga tccatcgccc gctcgaacca gcccgagaag
     1141 ccgaccaggg tgacgagcgc ggccgagacc aggaacgcgc cggtcgcctc ctcgatcgag
     1201 agcccggcgc cgctggtggc cagcagggcc gcccccggcg tggaccacgc ggtgacgatc
     1261 ggaacccggt agcgcagcga cagcaggctg cacgtgagcc ccatgccgag cccgagggcg
     1321 agcaaccacg agctgacctg ggcctccgtg gcgccgaccg ctcccgccgc ctggaagacg
     1381 atgaccgccg cgctggcgaa gcccaccagc acggtcacga acccggcgac gaccgccgaa
     1441 accgagagat cccggacgag cgacatgaaa aagaccccgc ccactatagc aatggtccgc
     1501 tccgggccga ccgggggatc gggtacactc cccgggatgc gcgcgtacct cctgtcgcgg
     1561 ctcgggcaga ccctgctggt cgtcttcctc tcgctgaccg ccgtcttctt cctggtccgg
     1621 ctcggcggcg acccggtcct gctgttcctg ccgatggaca tccaggccaa ggacctgaac
     1681 gagttccgcc agcgcctggg cttcaacgat ccgctcggcg tgcagtacgg ccgcttcatg
     1741 ggacgcgcgc tgcacggcga cttcggcgag tcgctgcgct acaagaacga cgcgctgcag
     1801 ctcgtgctcg ggcgcctacc cgcgacgctg tggctcgcgc tggccgcgct cctgctcacc
     1861 ttctgcgtgg cggtgccggt cggggtcttc tcggcggtcc ggcgcggctc gctcttcgac
     1921 ctgttcggca tgaccggcgc ggtgctcgga caggcggtgc ccggattctg gctcggcctg
     1981 atgatgatct acctcttctc ggtgcgactc ggctggctgc cgaccggggg catgggcggc
     2041 ccgctccact tcgtgatgcc gtgcgtggtg ctggcctcgt tctacgccgc ccgcatggcc
     2101 cggctcacgc gctcctcggt gctcgacacc ctcaacgagg agttcgtgct gaccgcgcgg
     2161 gccaaggggc tggcggagcg catcgtcatc ggcaagcaca ccctgaagaa ctcggcgatc
     2221 cccctcgtga ccctggccgg gctcgacacc ggtcagctcc tgggcggggc ggtcatcacc
     2281 gagaccatct tcgcgtggcc cgggctcggg cgcctcaccg tgcaggccct gctgaaccgc
     2341 gacttcccgg tggtgctggc cgcggtcttc gtgttctcgg tcacctacac cctgatcaac
     2401 ttcgcggtcg acctgctcta cggctggctc gatccgcgga cgcggcggca gcgggcgtga
     2461 gggtcttcgt cgcccgccac ggccgcggcg tgctcggcct gctcatcgcg acgctggtgc
     2521 tgctcgccgc cgtcgcggct ccggtcctgg tggcccagga cccggtgcag accgacttcg
     2581 cggccggcct caagccgccg ggcacgcccg gccacccgct cggcaccgac cagctcgggc
     2641 gcgatctgct ggcccgcgtg ctctacggcg cgcgggtggc gctcttcatc gggctctgct
     2701 gcgtgctgct caccgcgctg gtgggcgggc tgctcggcct cctcgccggc ttctacgagg
     2761 gctggccggg cgcggtgctc atgcggatcg ccgacgtgca gctctcgttc ccgttcatcc
     2821 tcctcgcgct gaccatcaac gcgatcgtgg gattggggct gcgcaacatc atcgtcagtt
     2881 tgtcagtcgc gggctgggtc gtgtatgctc gcgtggtccg gggggaggtg ctgtcggtga
     2941 agcagcgcga cttcgtgcac gcggcccagg cgctgggagt cgggcgcgcg cgcgtgctct
     3001 tccggcacgt gctccccaac gtggcaccct ccatcgtcat cgtggccagc ctccagttcg
     3061 cccagttcat cgtggccgag gccgcgatct cgttcctcgg cttcggggtg cagccgccga
     3121 ccccggcgtg gggcagcatg ctctcggaga gccgcgacta cctctacgtg gcgtggtggc
     3181 tggccgcctt cccgggcgcc gcgctcgcct tcaccgcgct cggcatcaat ctggtgggcg
     3241 actggctgcg cgacgtgctc gatccacgct tccgggtctg atggcggtcg tcatccgacc
     3301 ctgccgtccg gacgagggcc ccgcactgct ggacctctgg caccacgccg acacgagccc
     3361 gagccccacc gacacccccg agcaggtcga ccaggtgatc caggaccccg ccgcctcggt
     3421 gctggtggcg gtcgaccgcg atctgttggt gggctcgatc atcggcggct gggacggctg
     3481 gcgcggcaac atctaccgtc tggccgtgct gccatcgtac cgccggcgcg gcatcgcgcg
     3541 ggcgctggtc gcggccgcgg aggagcggct ggccgcgcgc ggcgcgcggc gcgtctccgc
     3601 gctcgtggag cacgatcacc cctgggcggt cggcttctgg gactcgctct acgagcccga
     3661 cccccgcatg atccgttacg tccggaaagt cgactcgaca aggagaccct catgacgcga
     3721 ttcacccgga tgctggccgc gctctcgctg ctgctgggcg tggcggtggc cgttcccctc
     3781 gccgatcccg ccggggccca ggaccgcgcg aagaccaagg agatcgtggt ggccttcgcg
     3841 gccgagccgc gcacgctcct gcccgacacc atcgtggact ggaccaccaa caacatggtc
     3901 gagcacatgt acgaccggct ggtggatcgc gatcccaaga gctacaaacc gtccccgatg
     3961 ctggccaccg cgtggaaggt ggtcaacgac accacctggg agttcacgct gcggcagggg
     4021 gtcaagttcc acaacggtga gcccttcaac gcccagagcg tgaaggccac catggagtac
     4081 atcaaggacc ccgccaacaa gacccactac ctgccgcgct ggtcccaggt gaaggaggtc
     4141 cagatcgtca acgactatac ggtgcgcttc gtcaccgaga
//
LOCUS       DATMCV010000400         4515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_684300, whole genome shotgun sequence.
ACCESSION   DATMCV010000400 DATMCV010000000
VERSION     DATMCV010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684300"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1014
                     /locus_tag="VNU03_09300"
     CDS             82..1014
                     /locus_tag="VNU03_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP74701.1"
                     /translation="MSSLQITLIVTVVLPALAIVLWPLVRGRSGDVVTTEERPRDDRR
                     LELEEEKVALYRALRELEFDHDAGHLSDADYDGLRERYETRAAALITDLDALGPAPAP
                     RVKPAAAAAEAGSARISWTRQPVVLAAGAVVLVIFGVVIGINASRFTEPDQTMTPPGS
                     RLPVQGDSPVGPPNVRLEPGKPIPPEMLAGMLNAARQSLFDGNYSQAIAAYQAVLKRD
                     ARNVDALTHMGLIVAIGGHADTAIETIDKALAIDPKYAPAYLYRGQVLFEQKQDYAGA
                     VKAWESFLKLVPKGEDHDRVVALVETAKSKRQAR"
     gene            complement(1093..4485)
                     /locus_tag="VNU03_09305"
     CDS             complement(1093..4485)
                     /locus_tag="VNU03_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP74702.1"
                     /translation="MIERAALFTEGALITGPVLEPLAAERPIPATPALDASASTVTPE
                     EAMRQYLRRVLEQSAGNISHAAARLGIARNTLYARLEKYRVRAPLPHPAPVRRAGRPA
                     LVATPAPPGPRIQWERRGIALLRVALVEPVGVEASLHTSRALEGVIDKLQMFGGRVEE
                     LTPTGVVTSFGVDRVDDAPRRAAHAAMVIHKGAARAREGTSRVPGVKIGIHVAQLLVG
                     RSASRIDIDADAKRAQWPVLDQLLQTIAPDETGASAAAAPFLERRFELVPIDGGAGGA
                     GQPYRLTGQERRGLGLWGTMTQFVGRHEELEVLRGRLAAAKHGQGQFVAVVGEPGVGK
                     SRLLWEFNHSAHVAGWLVLESGAVPYGNATPYLPVIDLLKVYCGIAERDDQRVIREKL
                     AGKLLALEREFEVALPAFLALLDLPVDDRAWQALDPAGRRRRTLDALKRLLLRESQVQ
                     PLALVFEDLHWIDGETQALLEGLIESLPGARLLLLVSHRSEYAHDWGCATSSTELRID
                     PLRREGAEELLEVLLGTDPGLGPLKRLLIERTEGNPLFVEESVRALVETGAVTGARGA
                     YRLARGLPPVHVPDTVQAILTARIDRLPPEGKRLLETAAVIGKDVPFALLQAVADESE
                     EMLGRGLGDLQTGEFLCESRRFPDLEYTFKHALTHEVAYGSLLHDRRRDLHTRIVGAI
                     ERSYQDRLTEHAERLAHHAMRGEMWEQAVTYLRQAGVKALVRSANREAVRGFEQALTA
                     LGHLPATRERLERGVDLLFDLRTALFPLGEFERIFGCLREAEGLARTLEDQPRLGQLS
                     VYLCHNFQMTGHSAEARAFGENTQAIAEALQDVPLQVTGNLYFGLACVGTGDHRRAED
                     LLLHVLQLLEAFPSQERFGQPGFPAVSVRGFLGWVLAERGKFEEGIMHGQAGLRLAET
                     LDHPYSVANVCWHLACLHIIRGEFSHVVGVLERGLALSREWNLTFFSALSTGSLGYAY
                     ALLGRVAEGIPLLEQALGANETMGHGVHQPLFLVYLGEAYVLADRLEDAFACAGRALT
                     LARERGQRGREARALRLLGEVAARRDPPEKAEACYRDALALAEELGMRPLVAHCRLGI
                     GKLLRRTGDRPRAQEHLTIAATMYREMDMRFWLEEAETRELT"
BASE COUNT          723 a         1621 c         1535 g          636 t
ORIGIN      
        1 gcggcccgcc gcgcccgagc ctcccccgct ggacccggcc atgcgcgcac gcatccggcg
       61 ggagatggcg gagcgcgagc cgtgagcagc ctgcagatca cgctgatcgt caccgtcgtc
      121 ctgcccgcgc tcgcgatcgt gctctggccg ctcgtgcgcg gccgcagcgg cgacgtcgtg
      181 acgacggagg agcgcccgcg ggacgaccgg cgcctcgagc tggaggagga gaaggtcgcc
      241 ctctaccgcg ccctgcgcga gctggagttc gaccacgacg cggggcatct ctcggacgcc
      301 gactacgacg gcctgcgcga gcgctacgag actcgcgccg ccgcgctgat cacggatctc
      361 gacgcgctgg ggccggcgcc cgcgccgcgc gtgaagcccg ctgcggcggc ggccgaggcg
      421 gggtcggcgc gcatctcctg gacgcggcag ccggtcgtgc tggcggccgg cgcggtcgtg
      481 ctggtgatct tcggcgtcgt catcggcatc aacgccagcc gcttcacgga gccggaccag
      541 accatgacgc cccccggctc gcgcctgccg gttcagggcg actccccggt gggcccgccc
      601 aacgtgcgcc tcgagccggg caagcccatc ccccccgaga tgctggccgg catgctcaac
      661 gccgcgcgcc agagcctctt cgacggcaac tactcgcagg ccatcgccgc ctaccaggcc
      721 gtgctcaagc gcgacgcccg gaacgtggac gcgctcaccc acatgggcct catcgtggcc
      781 atcggcggcc acgccgacac cgcgatcgag accatcgaca aggcgctcgc catcgacccc
      841 aagtacgcgc ccgcctacct ctaccgcggc caggtgctct tcgagcagaa gcaggactac
      901 gcgggagcgg tgaaggcgtg ggagagcttc ctcaagctcg tgcccaaggg cgaggaccac
      961 gaccgggtcg tggcgctggt ggagaccgcg aagagcaagc gccaggcgcg gtagaggcgg
     1021 gggaggtccg gggggacgac caaggcaccg cagacgatcg gccctccgct gcgaaccggc
     1081 cctcgtcctt cctcatgtca actcccgcgt ctccgcctcc tcaagccaga agcgcatgtc
     1141 catctcgcgg tacatcgtcg cggcgatagt caggtgctcc tgcgctcgcg gccggtcacc
     1201 tgtgcgtcgg aggagttttc cgatgccgag gcgacaatgg gcgacgagag ggcgcatccc
     1261 gagttcctcg gcgagcgcga gcgcgtcgcg gtagcaagcc tcagctttct cagggggatc
     1321 gcggcgggcg gcgacctccc cgaggagccg gagggcccgc gcctcccggc cgcgttgacc
     1381 gcgctcgcgg gcaagggtca gggctcgccc agcgcatgcg aaagcgtcct cgagccggtc
     1441 ggcgaggacg tacgcctcac ccaggtacac caggaaaagc ggctggtgaa ctccgtgtcc
     1501 catcgtttca ttggcgccca gcgcctgctc cagcaatggg atgccctccg caactcgccc
     1561 caagagcgcg tacgcgtagc ccaggctccc cgtggagagc gctgaaaaga aggtcaggtt
     1621 ccactcgcga gacagcgcca gcccgcgctc gagcacgccg acgacgtggc tgaactctcc
     1681 cctgatgatg tggagacagg cgagatgcca gcacacgttg gccacgctgt aggggtgatc
     1741 gagcgtctca gcgaggcgga ggcctgcctg gccgtgcatg atgccttcct cgaactttcc
     1801 ccgctcggcg agaacccaac ccaaaaagcc gcggaccgac acagccggaa atccgggctg
     1861 gccaaaccgt tcctgactcg gaaaagcctc cagcaactgc agaacatgca ggagaagatc
     1921 ctcggcccgt cgatggtccc ccgtcccgac gcaggccaat ccgaaataga gatttcccgt
     1981 cacctggagc ggaacatctt gaagcgcctc ggcaatggcc tgggtgttct cgccgaacgc
     2041 gcgcgcttcc gccgaatgcc cagtcatctg gaagttgtgg cacagataga cggacagctg
     2101 cccgagtcgc ggttgatcct cgagcgtcct ggccaggccc tccgcttcgc gaagacagcc
     2161 gaagattcgc tcgaactcgc cgagcggaaa gagtgcagtt ctcaggtcaa agagaagatc
     2221 gacgccccgc tccagcctct cgcgggtcgc gggcagatgc ccgagggccg tcagcgcttg
     2281 ctcgaagccg cgtaccgcct cccggttggc agaccgcacc aaggccttga cgccagcctg
     2341 ccggaggtac gtgaccgcct gctcccacat ctcaccacgc atggcatggt gggcgagccg
     2401 ctcggcgtgc tcggtcagcc ggtcctgata ggagcgttcg atggctccga cgatccgggt
     2461 gtggaggtcg cggcgccggt cgtggaggag actcccatag gccacctcat gcgtgagcgc
     2521 gtgcttgaag gtgtactcga ggtcggggaa ccggcgcgac tcgcaaagaa actcgcccgt
     2581 ctgaaggtcg cccagcccgc gaccgagcat ctcctcgctc tcgtccgcga ccgcctgaag
     2641 gagggcaaag gggacgtcct tcccgatgac tgcggcggtc tcgaggagcc gttttccttc
     2701 ggggggcagg cggtcgatgc gcgcggtcag aatggcctgt accgtgtcgg gtacgtggac
     2761 cggaggcagg ccacgcgcca gccggtaggc gcctcgtgcg cccgtgactg cgcccgtctc
     2821 gacgagcgcc cggacgctct cctcgacgaa gagcggattg ccctcggtgc gctcgatcag
     2881 aagccgcttg agcggaccca gcccggggtc ggtcccgagg agcacctcga gcagctcctc
     2941 ggcgccctcc cggcgcagcg ggtcgatccg cagctccgtg gaggaggtcg cgcagcccca
     3001 gtcatgcgca tactcggacc gatggctgac gagcagcaat agacgggccc cggggagact
     3061 ctcgatcagg ccctcgagca gcgcctgcgt ctcgccatca atccagtgca gatcctcgaa
     3121 cacgagcgcc agcggctgca cctggctctc gcgcaggagg aggcgcttga gggcgtccag
     3181 ggtccgccgc cgccgcccgg ccgggtcgag cgcctgccac gcccggtcgt ccacgggcag
     3241 atcgagcagc gccaggaatg cagggagggc gacctcgaac tcccgctcca gggccaggag
     3301 cttgccggcg agcttctcgc ggatgacgcg ctggtcatcc cgctcggcga tcccgcagta
     3361 gaccttgagc aggtcgatca cgggcagata gggcgtggcg ttgccgtagg gtacggcgcc
     3421 cgattccagc acgagccagc cagccacgtg cgccgagtgg ttgaactccc agaggaggcg
     3481 cgacttgccc actccgggct ccccgacgac cgccacgaac tggccctgcc catgcttcgc
     3541 cgcggccagg cggccgcgta agacctcgag ctcttcgtgc cggcccacga actgcgtcat
     3601 ggtgccccac agccccaacc cccggcgttc ttgcccggtc aggcgatacg gctggcccgc
     3661 gcccccggcg ccgccgtcga tcgggaccag ctcgaaccgg cgctccagga agggggcggc
     3721 ggcggcactc gcccccgttt cgtccggggc gatggtctgt agaagctgat cgaggactgg
     3781 ccattgggcg cgcttcgcat ctgcatcgat gtcgatccgg ctcgccgagc gtccgacgag
     3841 caactgcgcc acgtggatac cgatcttgac ccccggcaca cggctggtgc cctcacgggc
     3901 gcgcgcggcc cccttgtgaa tgaccatcgc ggcatgcgct gcgcgccgcg gcgcatcgtc
     3961 cacccgatcg accccgaacg aggtcacgac cccggtcggg gtcagctctt cgacccgccc
     4021 gccgaacatc tgcagcttgt cgatcacacc ttcgagggcc cggctcgtgt gcaaggaggc
     4081 ctcgacaccg accggctcga cgagggcgac gcggagcagc gcgatcccgc gacgttccca
     4141 ttgaatccga gggcccggcg gcgcaggggt cgcgacaagc gcgggccggc cggcgcggcg
     4201 gaccggcgcc gggtgaggga gcggggcgcg gacgcgatat ttctccaggc gggcatagag
     4261 cgtgttgcgc gcaatgccga gtcgcgccgc cgcgtgcgaa atattccccg cgctctgctc
     4321 cagcacccgc cggaggtact ggcgcatcgc ttcctccggc gtcacggtgc tcgcactcgc
     4381 gtcgagcgcc ggggtggcgg ggatcggcct ctccgccgcc agcggctcca gcacgggccc
     4441 cgtgatcaag gccccctcgg tgaacaacgc cgcgcgttcg atcacgttgg cgagttcgcg
     4501 gatattgccc ggcca
//
LOCUS       DATMCV010000401         4681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_756460, whole genome shotgun sequence.
ACCESSION   DATMCV010000401 DATMCV010000000
VERSION     DATMCV010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756460"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VNU03_09310"
     CDS             <1..406
                     /locus_tag="VNU03_09310"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HWP74703.1"
                     /translation="QHRTGQFADLIVSAVGRCPERAGLFAPSVTVKNIGPVPAGAFNV
                     AVDVGVFDSGGGAAVSTAAIVRVPGLAAGADTTVAFDRMQGVILRAGDRLTISAVADP
                     QQVPGSPLNVFGEILELDETNNSAVQNCTVPN"
     gene            complement(419..1567)
                     /locus_tag="VNU03_09315"
     CDS             complement(419..1567)
                     /locus_tag="VNU03_09315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP74704.1"
                     /translation="MQFVCFHLMPWPHLPADFEQRHESAWLDLPNTLYDPARGHALYN
                     DYLDQLVAAERLGFDVIGVNEHHQNAYGLMPSPNIMAASLIQRTSRIKILVLGNALPL
                     YDHPQRVAEELAMLDVLSNGRLIAGMVVGLGVEAFTYEINPTFIRERYREAHELIVRA
                     WTEPGPFHFEGKHYDFRWVNVWPRPIQKPHPPIWIPGSGSMETIRFVAERRYPWIALP
                     YSQFDVMKEHFEMLREYAATHCGYDVPPAQIGWPAMIYVGETDRQAREEFEPHFWYLA
                     KKGLRLPREYMFPPGHTSVESQLRPSHVKRKFISLLSSWQEAEEGTYAIVGSAETVRQ
                     KLREGIKAAGCGIIQGIFQVGSMPHDLTMRSMERFARDVMPALRAEFP"
     gene            complement(1587..2366)
                     /locus_tag="VNU03_09320"
     CDS             complement(1587..2366)
                     /locus_tag="VNU03_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP74705.1"
                     /translation="MAGSEQKTIAVGGKAIQLFTGGAGAPLLYLHGAGVYWWMPAHDL
                     LAARHRVHAPVHPGFGASQGYDEIEAMDDLVFHTLDVMDALGLDRADVVGLSLGGWLA
                     AELALRHPGRVSRLVLVDAAGTRVPGVERADLFMAGPARARGLLFADPESAVARQIVP
                     DTPPPDRLEAALRGREAAARLLWNPAGSYRKLTSRLGRIKAPTLVIWGAQDRVLPPAL
                     GQAYQRGIPGARLETLDGCGHLPPFEQPERFARLVLDFLQT"
     gene            complement(2393..3328)
                     /locus_tag="VNU03_09325"
     CDS             complement(2393..3328)
                     /locus_tag="VNU03_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74706.1"
                     /translation="MTRWPALVIGVALALAPLGVGRLYSAYYLNLLTWILIFALFAAG
                     LDLALGFGGLVSFGHAGFFGIGAYGAALALRHVAFSLWTALAVAIVAAALLAVVVGYF
                     AVHSRGVYMAMLTFAFAQLLYEVAIKWVAVTGGSDGLPGAGRPRVGWGALALDLSDKR
                     HMYWLVLAFVALGFALARRIVVSPFGQALIALRESEERARAIGIDVTRHRRVALVFSG
                     ALAGLAGGLFAIYQNFVSPELLFFALSGEVVVIALLGGMGTLYGSLVGAVIAIGLREI
                     LSTYTDNWLVVLGALYVACVMCFPQGLTRLFARTT"
     gene            complement(3325..4206)
                     /locus_tag="VNU03_09330"
     CDS             complement(3325..4206)
                     /locus_tag="VNU03_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74707.1"
                     /translation="MSLEAILTQLLMGLTVGVNLVLVATGLTLVFGMMGVINFAHSAL
                     FMLGAYAALMVSSRLGSFWLALAVAPPAIALLGAAVERWLLRPLRARYPLSHLLPLLL
                     TFGLSLFFEGSAKVLWGADIVSLDKPALLGGALALGRVTFPTYWLFALVAAAVLMVGV
                     WLVVERTSLGLSIRATTQDPMMAAVLGVDTQRLNTVVFALGSGLAAAAGVILGAMTSL
                     SPQMGAGIVLDAFIAIVIGGLGSFTGAIVGSLLLGQVQSFGVALLPAFHTPLVFLVMA
                     VVLVLRPSGLFGAAERR"
     gene            complement(4212..>4681)
                     /locus_tag="VNU03_09335"
     CDS             complement(4212..>4681)
                     /locus_tag="VNU03_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74708.1"
                     /translation="KQYADFGLKGPIKVIGPSNLVSEDVLSAQGDAALGVYSNSYYTP
                     SYDIPKNRAFVKACKDAGKEADHFLCAGYDAAQALFGALRETKGDVANKERLIQIITD
                     MRIDSPRGPLRFDPKNHNPISDIHLRTVQAGPLRHVVVDVLKDVKHPDMGCVL"
BASE COUNT          763 a         1630 c         1698 g          590 t
ORIGIN      
        1 ccagcaccgg accgggcagt tcgcggacct catcgtgagc gcggtcggtc gctgcccgga
       61 gcgggcgggc ctgttcgccc ccagcgtcac ggtcaagaac atcggcccgg tgcccgcggg
      121 agccttcaac gtggcggtcg acgttggggt cttcgacagc ggcggcggcg ccgcggtctc
      181 gaccgcggcg atcgtgcgcg tcccgggcct cgccgcgggc gcggacacca ccgtggcctt
      241 cgaccgcatg cagggggtga tcctgcgggc cggggatcgg ctcaccatct ccgcggtcgc
      301 cgatccccag caggtgcccg gcagcccgct caacgtgttc ggcgagatcc tcgagctgga
      361 cgagaccaac aacagcgcgg tccagaactg cacggtaccg aactagcccg gcgccgcctc
      421 aggggaactc ggcccgcagc gcgggcatca cgtcccgcgc gaatcgctcc atgctgcgca
      481 tcgtgaggtc gtgcggcatg ctccccacct ggaagatgcc ctggatgatg ccgcagccgg
      541 ccgccttgat gccctcgcgg agcttctgcc gcaccgtctc cgcgctgccc acgatggcgt
      601 aggtcccctc ctccgcctcc tgccacgacg agagcagcga gatgaacttg cgcttgacgt
      661 ggctcgggcg gagctgcgac tcgaccgagg tgtggccggg cgggaacatg tactcgcgcg
      721 ggaggcggag gcccttcttg gccaggtacc agaagtgggg ctcgaactcc tcgcgcgcct
      781 ggcgatcggt ctcccccacg tagatcatgg ccggccagcc gatctgggcg ggcggcacgt
      841 cgtagccgca atgggtcgcc gcatactcgc gcagcatctc gaagtgctcc ttcatgacgt
      901 cgaactgcga gtacggcagc gcgatccacg ggtaccgccg ctcggccacg aagcggatgg
      961 tctccatgct gcccgagccc gggatccaga tcggcgggtg gggcttctgg atcggccggg
     1021 gccagacgtt cacccagcgg aagtcgtagt gcttgccctc gaagtggaac gggccgggct
     1081 cggtccacgc gcgcacgatc agctcgtggg cctcgcgata gcgctcgcgg atgaaggtgg
     1141 ggttgatctc gtaggtgaag gcctcgaccc cgagccccac caccatgccg gcgatcagcc
     1201 gcccgttgga cagcacgtcg agcatcgcca gctcctcggc cacccgctgg ggatgatcgt
     1261 agagcggcag cgcgttgccg aggaccagga tcttgatgcg gctcgtgcgc tggatcagcg
     1321 aggcggccat gatgttcggc gacggcatca ggccgtacgc gttctggtgg tgctcgttga
     1381 cgccgatcac gtcgaagccg aggcgctccg ccgcgacgag ctgatccagg tagtcgttgt
     1441 agagcgcgtg cccgcgggcc ggatcgtaga gcgtgttggg caggtcgagc caggcggact
     1501 cgtggcgctg ctcgaagtcg gcgggcagat ggggccacgg catcaggtgg aagcagacga
     1561 attgcatggc ggcgtctcct cggggctcag gtctgcagga agtcgagcac gagacgcgcg
     1621 aagcgctcgg gctgctcgaa gggcggcagg tggccgcagc cgtccagggt ctcgagccgc
     1681 gcgccgggga tcccgcgctg gtaggcctgg ccgagcgcgg gcggcagcac ccggtcctgc
     1741 gcgccccaga tcaccagggt gggcgccttg atccggccga gccggctcgt gagcttacgg
     1801 tacgacccgg ccgggttcca cagcaggcgc gcggcggcct cgcgtccgcg cagcgcggcc
     1861 tcgagccgat cgggcggcgg cgtgtccggc acgatctggc gcgccaccgc cgactcgggg
     1921 tcggcgaaca ggaggccacg ggcccgcgcc ggaccggcca tgaagaggtc cgcgcgttcg
     1981 accccgggca cccgcgtgcc cgccgcgtcc accagcacga ggcggctcac ccggcccgga
     2041 tgccgcaggg ccagctccgc ggcgagccag ccgccgagcg atagaccgac cacgtccgcc
     2101 cgatcgagcc cgagcgcgtc catcacgtcg agggtgtgga agaccaggtc gtccatcgcc
     2161 tcgatctcgt cgtagccctg cgaggccccg aaacccgggt gcaccggcgc gtgcacgcgg
     2221 tgccgcgccg cgaggaggtc gtgcgccggc atccaccagt agacgccggc gccgtggagg
     2281 tagaggaggg gcgcgcccgc tcccccggtg aagagctgga tggccttgcc gccgaccgcg
     2341 atggtcttct gttccgatcc cgccattcga gcgctcccgt gtgacgcggc tctcaggtcg
     2401 tccgggcgaa caggcgcgtg agcccctgcg ggaagcacat cacgcaggcc acgtagagcg
     2461 cgccgaggac gaccagccag ttgtcggtgt aggtggagag gatctcgcgg agaccgatgg
     2521 cgatgacggc ccccaccagc gagccgtaga gggtgcccat gccgcccagc agcgcgatca
     2581 ccaccacctc gcccgagagc gcgaagaaga gcagctccgg ggacacgaag ttctggtaga
     2641 tcgcgaacag gccgccggcc agcccggcca gcgcccccga gaacaccagc gccacgcgcc
     2701 ggtgccgggt gacgtcgatg ccgatcgcgc gcgcccgctc ctcgctctcc cgcagcgcga
     2761 tcagggcctg gccgaagggc gagaccacga tgcgccgggc cagcgcgaac cccagcgcca
     2821 cgaaggcgag caccagccag tacatgtggc gcttgtccga gaggtcgagc gccaaggcgc
     2881 cccacccgac acgcggccgg cccgcgcccg gcaggccgtc gctaccgccg gtgaccgcca
     2941 cccacttgat cgccacctcg tagaggagct gggcgaaggc gaaggtgagc atcgccatgt
     3001 acaccccgcg cgagtggacc gcgaaatagc cgaccaccac cgcgaggagc gcggccgcca
     3061 cgatcgccac cgcgagcgcg gtccagagcg agaacgccac gtggcggagg gccagcgcgg
     3121 ctccgtaggc cccgatgccg aagaacccgg cgtgcccgaa cgagacgagc ccgccgaagc
     3181 cgagggccag gtcgagcccg gccgcgaaga gggcgaagat caggatccag gtgagcaggt
     3241 tcaggtagta ggcggagtag agacgcccca ccccgagggg ggccagcgcc agggccacgc
     3301 cgatcaccag cgccggccag cgggtcatcg gcgctccgcc gcgccgaaga ggccgctcgg
     3361 ccgcaggacc agcaccaccg ccatgaccag gaacacgagc ggggtgtgga aggcaggcag
     3421 cagggccacg ccgaaggact ggacctggcc cagcaggagc gagccgacga tggcgccggt
     3481 gaagctgccg agcccgccga tgacgatggc gatgaaggcg tcgagcacga tgcccgcccc
     3541 catctgcggc gagaggctcg tcatcgcgcc caggatcacc ccggccgcgg cggccagccc
     3601 cgagccgagc gcgaacacca ccgtgttgag ccgctgggtg tcgacgccga gcaccgcggc
     3661 catcatcgga tcctgggtgg tggcccggat cgagaggccg agcgaggtgc gctcgaccac
     3721 gagccacacg ccgaccatga gcaccgcggc cgcgaccagc gcgaagagcc agtaggtggg
     3781 gaacgtgacc cggccgagcg ccagcgcgcc gcccaggagc gcgggcttgt cgagcgagac
     3841 gatgtccgcg ccccagagga ccttggccga gccctcgaag aagagcgaca ggccgaacgt
     3901 gagcagcagc ggcaggaggt gcgagagcgg atagcgcgcg cgcagcgggc gcaggagcca
     3961 gcgctccacc gccgcgccca gcagcgcgat cgcgggcggc gcgacggcga gggcgagcca
     4021 gaagctgccc aggcggctcg agaccatcag cgcggcgtag gcgcccagca tgaagagcgc
     4081 gctgtgggcg aagttgatca cccccatcat cccgaacacg agggtgagcc cggtggcgac
     4141 cagcacgagg ttcaccccca ccgtgagccc catcaggagc tgggtcagga tcgcctcgag
     4201 cgacatcggg gctacagcac gcagcccatg tccgggtgct tcacgtcctt gaggacgtcg
     4261 accaccacgt ggcgcagcgg tccggcctgc accgtgcgca ggtggatgtc ggagatcggg
     4321 ttgtggttct tggggtcgaa gcggagcggg ccgcgcgggc tgtcgatccg catgtcggtg
     4381 atgatctgga tcagccgctc cttgttcgcc acgtcgccct tggtctcgcg cagggcgccg
     4441 aagagggcct gggccgcgtc gtagcccgcg cacaggaagt ggtcggcctc cttgcccgcg
     4501 tccttgcacg ccttcacgaa ggcgcggttc ttggggatgt cgtagctcgg ggtgtagtag
     4561 gagttggagt agacgccgag cgcggcgtcg ccctgggcgc tcagcacgtc ctccgagacc
     4621 aggttcgacg gtccgatgac cttgatcggc cccttcagcc cgaagtcggc gtactgcttg
     4681 a
//
LOCUS       DATMCV010000402         3394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_957299, whole genome shotgun sequence.
ACCESSION   DATMCV010000402 DATMCV010000000
VERSION     DATMCV010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957299"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VNU03_09340"
     CDS             <1..663
                     /locus_tag="VNU03_09340"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP74709.1"
                     /translation="IGLLVFVGLPGRIGGPSGRDVTSVVASLGAGFREAWREPTVRAV
                     LLITVMMNVLFFPYQHMLPVFARHVLAVGPEWLGGLVAADGLGALLGALAIAGRRGFL
                     PHGRIFGVAVLTAPFLLLGFTSLRWPWACLPVLVLIGACESAFAAMQSTLVLLQAPEA
                     SRGRAMGILSACIGTQPAGTLWIGFLAGGIGVPAAMAINGMAALVAMLPAAWPLARRG
                     RI"
     gene            771..1559
                     /locus_tag="VNU03_09345"
     CDS             771..1559
                     /locus_tag="VNU03_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74710.1"
                     /translation="MKMMRVVLTLILVAAVSLSLPGATSLAQEVLTNDSVIQMVKAGL
                     PEAVVIGKIKSTATKFDLKTDSLVSLKKAGVSDKVLEAMVAAGSGAAPSTAAAPTPAP
                     PAPALAAGALKNQDVIYQLVAGKYVELFATSANLETNMAFFQSKSEVVLEGKKAQYRT
                     SEKQPVFLSTYSSTDAPLVRLKPGDEHNDRNLKIGSGAFMPFGGTQKMGVRNEDKIPV
                     TIERDARGFYKVTPAAALAPGEYGFILATGFGAGSGKVYDFGVD"
     gene            1627..2736
                     /locus_tag="VNU03_09350"
     CDS             1627..2736
                     /locus_tag="VNU03_09350"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP74711.1"
                     /translation="MSERTEEERRLPEPVLRALHDASLFRLLLPAFLDGGEIDPVTFV
                     LVIEEVAKHDASAAWCLCQASGCSMTAAYLPTEVGREIFGDRRAVLAWGPSNDARAVA
                     VAGGYRIDGTWSFGSGCRHATWLGGYCPIQGSDGKTRRRPDGTPDGRTMLFPAGQATI
                     VDVWHVTGLRGTGSDAFTVTDLFVPDAHTVARDDPAERRHPGLLYCFPSGSLYAAGFS
                     GVAMGIARSSLDAFVELARDKEPRSSKRQLRDSGVIQFQVAEAEAKLGAARVFLHDAL
                     ARIWAEVSRSGLPLSLEQRVQIRLASTYGIHQAKQVVDTVHHAAGSSAIFTSSAFERR
                     FRDIHTVTQQLQGRQAHLETVGRYLLGLEPDQTFL"
     gene            2799..>3394
                     /locus_tag="VNU03_09355"
     CDS             2799..>3394
                     /locus_tag="VNU03_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP74712.1"
                     /translation="MDSGIEYQGLVLGHLDSLYNYALVLTRRSEDAEDLLQEVLVRGF
                     QGFASFDQTLSFKGWMFTIIKNAYIDRFRRQRRLPPEEPLWASGEEPVLSLDNPLCSV
                     PLAPEDLLLRREAIEQVREAIRRLPEEMREVVELRDVEGLSYREIAHVVNRPIGTVMS
                     RLYRGRNLLRTCLVELRRSVEQSPPRPADQASKAKAHDR"
BASE COUNT          481 a         1213 c         1181 g          519 t
ORIGIN      
        1 atcggcctgc tcgtcttcgt gggcctgccc gggcgcatcg gcgggccgag cgggcgcgac
       61 gtcacctcgg tggtggccag cctcggcgcc ggcttccgcg aggcgtggcg cgagcccacc
      121 gtgcgcgcgg tgctgctgat cacggtgatg atgaacgtgc tcttcttccc ctaccagcac
      181 atgctgccgg tcttcgcgcg ccacgtgctc gcggtgggcc cggagtggct cggcggcctg
      241 gtggccgcgg acgggctcgg cgcgctgctg ggggcgctcg ccatcgcggg acgccgcggc
      301 ttcctgccgc atgggcgcat cttcggggtg gcggtcctga ccgcaccgtt cctgctgctc
      361 ggcttcacga gcctccgctg gccatgggcc tgcctgccgg tgctggtgct gatcggggcg
      421 tgcgagtcgg ccttcgcggc catgcagagc acgctggtgc tcctgcaggc cccggaggcg
      481 agccgcgggc gggccatggg catcctctcg gcctgcatcg gcacccagcc ggccggcacc
      541 ctctggatcg gcttcctcgc cggagggatc ggggtcccgg cggccatggc gatcaatggt
      601 atggccgcgc tcgtcgcgat gctgccggct gcctggccgc tggcccgccg gggccggatt
      661 tgaccgctat tgaccgacct tacgaagtac cctaagttta tggccggtta ccgtgaccgc
      721 cggggtgggc ccggccggtg ccaaccgttt cccgtcgccc aggaggcctc atgaagatga
      781 tgcgtgtggt cctgaccctg atcctggtgg ccgccgtcag cctgtcgctg ccgggcgcca
      841 cctcgctcgc ccaggaagtg ctgaccaacg actcggtgat ccagatggtc aaggccggcc
      901 tgcccgaggc ggtcgtcatc ggcaagatca agtcgaccgc gaccaagttc gatctcaaga
      961 ccgactcgct ggtgagcctg aagaaggccg gcgtctccga caaggtgctc gaggccatgg
     1021 tggccgcggg ctccggcgcg gcgccgagca ccgccgcggc gccgacgccc gcgccgccgg
     1081 cgcccgcgct ggcggcggga gcgctcaaga accaggacgt gatctaccag ctcgtcgccg
     1141 gcaagtacgt cgagctgttc gcgacctcgg ccaacctcga gaccaacatg gccttcttcc
     1201 agagcaagag cgaggtggtg ctggaaggca agaaggccca gtaccggacc agcgagaagc
     1261 agccggtgtt cctcagcacg tactcctcga ccgacgcgcc actggtgcgg ctcaagcccg
     1321 gtgacgagca caacgaccgc aacctcaaga tcggcagcgg agcgttcatg ccgttcggcg
     1381 ggacccagaa gatgggcgtg cgcaacgagg acaagatccc ggtgacgatc gagcgggatg
     1441 cgcgcggctt ctacaaggtg acgccggccg ccgcgctcgc gcccggcgag tacggcttca
     1501 tcctcgccac cgggttcggg gcgggctcgg gcaaggtcta cgacttcgga gtggactaat
     1561 acggatccgc accacgccgc gggcccgctc gagcgggccc gcgcgctggc cccgctcctc
     1621 gaggacctga gcgagcgcac cgaggaggag cgacggctcc ccgagccggt gctgcgggcg
     1681 ctccacgacg cctccctctt ccgcctcctc ctgcccgcgt tcctggacgg gggcgagatc
     1741 gacccggtca ccttcgtcct ggtgatcgag gaggtcgcca agcacgacgc gagcgccgcg
     1801 tggtgtctct gccaggcctc cggctgctcg atgaccgcgg cctacctgcc caccgaggtc
     1861 gggcgcgaga tcttcggcga ccggcgcgcg gtgctggcct gggggccgag caacgacgcc
     1921 cgcgcggtgg cggtcgccgg cggctaccgc atcgacggca cgtggagctt cggcagcggc
     1981 tgccggcacg cgacctggct cggcggctac tgcccgatcc aggggtccga cggcaagacg
     2041 cgccgccgcc cggacggcac gcccgacggg cgcaccatgc tcttcccggc cgggcaggcg
     2101 accatcgtcg acgtctggca cgtgaccggg ctgcgcggga ccggcagcga cgcgttcacg
     2161 gtcaccgatc tcttcgtccc ggacgcgcac accgtggcgc gcgacgatcc cgccgagcgc
     2221 cgacatcccg gcctgctcta ctgcttcccc tccgggagcc tctacgcggc cggcttctcc
     2281 ggcgtggcca tgggcatcgc gcggagcagc ctcgacgcct tcgtcgagct ggcccgcgac
     2341 aaggagccgc gctcgtccaa gcggcagctg cgcgacagcg gggtcatcca gttccaggtg
     2401 gccgaggccg aggcgaagct gggcgcagcg cgcgtcttcc tgcacgacgc gctcgcccgc
     2461 atctgggccg aggtgagccg gtcgggcctg ccgctcagcc tggagcagcg ggtgcagatc
     2521 cggctggcct ccacctacgg gatccatcag gccaagcagg tggtggacac cgtgcaccac
     2581 gccgccggct cgagcgccat cttcacgagc agcgccttcg agcgccgctt ccgggacatc
     2641 cacaccgtga cccagcagct ccagggccgc caggcccacc tcgagaccgt cggccgctac
     2701 ctcctcggcc tcgagcccga ccagaccttt ctgtagtttc cgccctctgc cgggaatatc
     2761 ggaccttccg gggtcgtagt agcgggtgag gaggccctgt ggacagcggc atcgaatatc
     2821 agggtctggt actaggtcat ctggattcgc tgtacaacta cgcgctggtc ttgacgcgtc
     2881 gctccgagga cgccgaggac ctgctgcaag aggtcctcgt ccgtggattt cagggcttcg
     2941 cctcgttcga ccagacgctg agcttcaagg gctggatgtt caccatcatc aagaacgcat
     3001 acatcgaccg attccgccgc cagcgcaggc tgccgcccga ggagccgctc tgggcctccg
     3061 gcgaggagcc ggtgctctcg ctcgacaacc cgctctgctc ggtgcccctg gccccggagg
     3121 atctgctgtt gcgccgggaa gccatcgagc aggtccggga ggcgatccgc cggctgccgg
     3181 aggagatgcg cgaggtcgtg gagctgcgcg acgtcgaggg tctctcctat cgcgagatcg
     3241 cccacgtcgt gaaccggccg atcggcaccg tgatgtcacg gctctatcgg ggacgcaacc
     3301 tgctccggac ctgcctggtc gagctcaggc ggtccgtcga gcagtcgccg ccccgcccgg
     3361 cggatcaggc gagcaaggca aaggcacatg atcg
//
LOCUS       DATMCV010000403         3843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_488413, whole genome shotgun sequence.
ACCESSION   DATMCV010000403 DATMCV010000000
VERSION     DATMCV010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488413"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VNU03_09360"
     CDS             <1..1341
                     /locus_tag="VNU03_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HWP74713.1"
                     /translation="MGISETHQVLTLNRLREKVLLRTDGGIKTGRDVVIAAMLGAEEY
                     GIGTASLIAMGCIMVRQCHSNTCPVGVCTQDPKLRAKFAGRAEFVENFFRYIAEEVRE
                     HMARLGFRTMDEMIGRVDRIDVAPAVEHWKARGLDFSAILHRPAVGPEVAVRRVVAQD
                     HGLDRSLDMTTLLPLCRPALERREPVDIRLPIQNVNRTVGTILGSEITTRYGADGLPD
                     DTIRIHFTGSAGQSFGAFLPRGVSLTLEGDANDYIGKGLSGGKLVVFPPREATFVAEE
                     NILVGNVVLYGATGGEAYFRGVAGERFAVRNSGTLAVVEGVGDHGCEYMTGGRVVVIG
                     RTGRNFAAGMSGGLAFVLDETGDFKARCNLGMVDLEPLVDTEDVELVKDLLARHIRYT
                     QSPVAARLLVDWKRSQARFVKVIPQDFKRVMAAIKKAQETGMSADEAVMASAHN"
     gene            1345..2793
                     /locus_tag="VNU03_09365"
     CDS             1345..2793
                     /locus_tag="VNU03_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HWP74714.1"
                     /translation="MGKVTGFLEFKRDKQPYRPVEERVRDWKQVMLPWPGDGLRKQGA
                     RCMDCGIPFCHQGCPLGNLIPDWNDLVYRDRWREAIDRLHATNNFPEFTGTLCPAPCE
                     GSCVLGINDDPVTIKGIEISIIDRAFAEGWVGPEPPAVRTGKTVAVIGSGPAGLAAAQ
                     QLCRAGHAVTVFERADRIGGLLRYGIPEFKMEKRVLDRRLAQMEAEGVRFVVNAHIGV
                     DRPVEALRREFDALVLCGGACAPRDLPIPGRDLDGVHFAMDYLTQQNRRCEGEAVPDE
                     TFVTAQGKRVVIIGGGDTGADCLGTTHRQGALEVHQLEILPRPPDQRAEDNPWPQWPN
                     IYRVSSAHAEGGERVYSVSTLRFVGDERGRVKGLEIARVEPVREGGRLTFREVPDSTS
                     TLPCELVLLAMGFVGPERPGLLQQLGVALTERGNVKRDPSWMTSVPGVFTAGDMQRGQ
                     SLIVWAIAEGRSAARGVDRFLMGRSDLPAPLA"
     gene            complement(2801..3328)
                     /locus_tag="VNU03_09370"
     CDS             complement(2801..3328)
                     /locus_tag="VNU03_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74715.1"
                     /translation="MKASLCNGGWRRRLAATVIGMCLSTTVAHAQPAQAPGRLDAAGL
                     RAALTAVGYQPREVKNDAGVEYEIVLRLRTGAAVTTRAALSKDGTLVWLVAWLRKLPP
                     GRTISGNAILDMLVENDAIGPTHFSYNERVRWFFLNSPVVNQDLTPERLRGEIDHVAN
                     VAARTEALWDFERWR"
     gene            3331..3828
                     /locus_tag="VNU03_09375"
     CDS             3331..3828
                     /locus_tag="VNU03_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP74716.1"
                     /translation="MPLTRSERERLIQQYADGPARLEGALAAVPAAARQWRPAPGEWS
                     AHEVACHCADSEVNAAARIRYVVCEPQPVVQAYDESVWATTLDYHGHPLEPALAAVRA
                     VRAHTTALIRRLPESAWSREGRHTGSGRYTAEDWLGIYAEHLEIHARQIEANVAAWQA
                     AGRPS"
BASE COUNT          551 a         1384 c         1384 g          524 t
ORIGIN      
        1 atgggcatct cggagaccca tcaggtgctg acgctcaatc gcctgcgcga gaaagtgctg
       61 ctgcgcaccg acggcggcat caagaccgga cgcgacgtgg tgatcgccgc catgctgggg
      121 gccgaggaat acggcatcgg caccgcctcg ctgatcgcca tgggctgcat catggtgcgc
      181 cagtgccatt ccaacacctg cccggtcggc gtctgcacgc aggatccgaa gctgcgcgcc
      241 aagttcgccg ggcgcgcgga gttcgtggag aacttcttcc gctacatcgc ggaggaggtg
      301 cgcgagcaca tggcgcgcct cggcttccgc accatggacg agatgatcgg ccgcgtcgac
      361 cgcatcgacg tcgcgcccgc ggtcgagcac tggaaggccc gcggcctcga cttctcggcg
      421 atcctccacc gccccgcggt ggggcccgag gtggcggtcc ggcgggtggt cgcgcaggat
      481 cacggcctcg accgctcgct cgacatgacc accctgctgc cgctctgccg gcccgcgctc
      541 gagcggcgcg agccggtcga catccggctg cccatccaga acgtgaaccg cacggtgggc
      601 acgatcctgg gctcggagat caccacccgg tacggggccg acggcctgcc cgacgacacc
      661 atccggatcc acttcaccgg ctccgccggg cagagcttcg gcgccttcct gccccggggc
      721 gtgagcctca ccctcgaggg cgacgccaac gactacatcg gcaagggcct ctccggcggc
      781 aagctcgtgg tcttcccgcc gcgcgaggcc accttcgtgg ccgaggagaa catcctggtc
      841 ggcaacgtgg tgctctacgg ggccaccggc ggcgaggcct acttccgcgg ggtcgccggc
      901 gagcgcttcg cggtccggaa cagcgggacc ctcgcggtgg tggagggcgt gggcgaccac
      961 ggctgcgagt acatgaccgg cgggcgggtg gtcgtcatcg gccgcaccgg gcgcaacttc
     1021 gcggccggca tgtcgggcgg tctcgccttc gtgctggacg agaccggcga cttcaaggcg
     1081 cgctgcaacc tgggcatggt cgacctggag ccgctcgtcg acaccgagga cgtggagctg
     1141 gtcaaggacc tgctcgcgcg ccacatccgc tacacgcaga gcccggtggc cgcgcgactg
     1201 ctggtggact ggaagcggag ccaggccagg ttcgtgaagg tgattcctca agatttcaag
     1261 cgggtgatgg ccgcgatcaa gaaggcccag gagaccggca tgtcggccga cgaggccgtg
     1321 atggcgagcg cgcacaacta ggtcatgggc aaggtcaccg gcttcctcga gttcaagcgg
     1381 gacaagcagc cctaccgccc ggtggaggag cgcgtccgcg actggaagca ggtgatgctg
     1441 ccgtggccgg gcgacggcct gcgcaagcag ggcgcgcgct gcatggactg cggcatcccc
     1501 ttctgccacc agggctgccc gctcggcaac ctgatcccgg actggaacga tctcgtctac
     1561 cgcgaccggt ggcgcgaggc catcgatcgc ctgcacgcga ccaacaactt cccggagttc
     1621 accgggaccc tttgcccggc cccctgcgag ggctcctgcg tgctcgggat caacgacgac
     1681 ccggtcacca tcaagggcat cgagatctcg atcatcgacc gcgccttcgc ggagggctgg
     1741 gtcgggccgg agccgcccgc ggtccggacg ggcaagacgg tcgcggtgat cggctcgggt
     1801 cccgccggcc tcgcggcggc ccagcagctc tgccgcgccg gccacgcggt gaccgtcttc
     1861 gagcgggccg atcgcatcgg cggcctgctc cgctatggca tccccgagtt caagatggag
     1921 aagcgggtcc tggaccggcg gctcgcgcag atggaggcgg agggcgtgcg cttcgtggtc
     1981 aacgcccaca tcggggtgga ccggcccgtc gaggcgctgc ggcgggagtt cgacgccctc
     2041 gtgctctgcg gcggggcctg cgccccccgc gacctgccga tcccgggccg ggacctcgac
     2101 ggggtccact tcgcgatgga ctacctgacc cagcagaacc gccgctgcga gggcgaggcg
     2161 gtacccgacg agaccttcgt caccgcccag ggcaagcgcg tggtcatcat cggagggggc
     2221 gacaccgggg ccgactgcct gggcaccacg caccgccagg gcgcgctcga ggtgcaccag
     2281 ctcgagatcc tcccgcgccc gcccgaccag cgcgcggagg acaacccgtg gccgcagtgg
     2341 cccaacatct accgggtctc ctcggcccac gccgagggcg gcgagcgggt ctactcggtc
     2401 tcgaccctcc gcttcgtggg cgacgagcgc ggccgggtga agggcctcga gatcgcgcgg
     2461 gtcgagccgg tgcgcgaggg gggccggctc acgttccggg aggtcccgga ctccacgtcg
     2521 acgctgccgt gcgagctggt gctgctggcc atgggcttcg tcgggcccga gcggcccggc
     2581 ctcctgcagc agctcggggt ggcgctcacc gagcgcggca acgtgaagcg cgacccgagc
     2641 tggatgacca gcgtgccggg cgtgttcacc gccggcgaca tgcagcgtgg ccagtcgctg
     2701 atcgtctggg cgatcgcgga aggacgcagc gccgcccgtg gagtggaccg cttcctcatg
     2761 ggccggtccg acctgcccgc ccccctggcc tgagcggcgc ctagcgccag cgctcgaagt
     2821 cccagagcgc ctcggtgcgc gccgcgacgt tcgccacgtg atcgatctct ccccgcagcc
     2881 gctcgggcgt cagatcctgg ttgaccaccg ggctgttcag gaagaaccag cgcacccgct
     2941 cgttgtacga gaagtgggtc ggcccgatcg cgtcgttctc gacgagcatg tcgaggatcg
     3001 cgttgccgct gatggtccgt cccggcggca gcttgcggag ccaggccacc agccagacga
     3061 gggtgccgtc cttggagagg gccgcccggg tggtcaccgc cgcgccggtg cgcaggcgca
     3121 ggacgatctc gtactccacg cccgcgtcgt tcttgacctc gcgcggctgg tagccgaccg
     3181 cggtgagcgc cgcgcgcagc ccggccgcgt cgaggcgacc cggcgcctgc gcgggctggg
     3241 cgtgggcgac cgtcgtcgac aagcacatcc cgatcaccgt ggcggccagc cggcgtcgcc
     3301 acccaccatt gcacagggag gccttcatcg atgcccctca cccgttccga gcgagagcgc
     3361 ctgatccagc agtacgccga cggccccgcg cgcctggagg gcgccctggc cgcggtgccc
     3421 gcggcggcgc gccagtggcg ccccgcgccc ggcgagtggt ccgcgcacga ggtggcctgc
     3481 cactgcgcgg actcggaggt caacgcggcg gcccgcatcc gctacgtggt gtgcgagccc
     3541 cagcccgtgg tccaggccta cgacgagagc gtgtgggcga cgaccctcga ctaccacggc
     3601 catcccctgg agccggcgct cgccgcggtc cgggcggtgc gcgcccacac caccgccctc
     3661 attcgtcgcc tgccggagtc cgcgtggagc cgcgagggtc gccacaccgg gtcgggccgg
     3721 tacaccgcgg aggactggct cggcatctac gccgagcacc tggaaatcca cgcccggcag
     3781 atcgaggcca acgtggccgc gtggcaggcg gcgggccgcc ctagctgagg ggacggacga
     3841 tgg
//
LOCUS       DATMCV010000404         3548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_888279, whole genome shotgun sequence.
ACCESSION   DATMCV010000404 DATMCV010000000
VERSION     DATMCV010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888279"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VNU03_09380"
     CDS             <1..287
                     /locus_tag="VNU03_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HWP74717.1"
                     /translation="GGAAANDFLCQFQADVLGVEVLRPSVTETTGLGAAYLAGVGAGL
                     WKSRDLAGRWKLEREFTPAMSPAAREVGYAGWRRAVERSRGWIEPDRRDA"
     gene            284..1420
                     /locus_tag="VNU03_09385"
     CDS             284..1420
                     /locus_tag="VNU03_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP74718.1"
                     /translation="MMRSTVTRAALALIAALSVLAPIGAPARAQAPIEGELNLITPVS
                     KFIHDAALKAFADYAKEKWNVTVKVSAIPAGTPVAYGRITEWKGKPEVDIFWGGESAL
                     FEKLAEQKLLQKVEISKEAWESIPAAIGKPKPIPLKDRDGYWVGTTLEPYGIVYNPKR
                     LQRLGVAPLKDWDDLLNPKLKGEVAQCAPTRSSSSNATYEVILSLQGEEPGWGWLRKL
                     AANTGHFTARSRDVPTVVAKGEFATGFAVPSYMAFEEKLAGFDIKYVAPKNAFVTPEP
                     MAILAGARNPRAARAFIEFLLTERGQKVFMERGLFSVNPKYKVQGAPGSTAEIAVEFT
                     GGVRSYFDGDVANVYDEAVAARRSEALKTRFRSDIEAKWDELKK"
     gene            1455..2528
                     /locus_tag="VNU03_09390"
     CDS             1455..2528
                     /locus_tag="VNU03_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011249527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74719.1"
                     /translation="MIVTIQGVVKRFGVVTAVDAVDLTVADGELFTLLGPSGCGKTTL
                     LRLLAGFYQPDGGTIRFGDRVVNGLAPYERRIGMVFQNYALWPHMTVAENIGYGLRLR
                     RLPGEEVARRLQEGLRKVNLTGFEARYPGQLSGGQQQRVALARALVLNPDILLLDEPL
                     SNLDAKIRIQVRAEIRRLQRELAITTIYVTHDQEEALSLSDRVAVMRDGRVLQVGPPR
                     DLYERPRTRFVADFVGTNNLVPGEVESRAAEGVDMVVRTALGPLRAIANGAVSARCVL
                     AVRPENVALGPAATGEGNRVAGRVSLVSYLGSTVRYDVETEAGLVLKADIRDAWHHDP
                     LPVGRPVTLSFPSSVTLAVADDA"
     gene            2521..>3548
                     /locus_tag="VNU03_09395"
     CDS             2521..>3548
                     /locus_tag="VNU03_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP74720.1"
                     /translation="MRSPRLALGVGLIWAFLILFLVYPLGRIFYDAVTDEAGRWTLAN
                     FHEFFTDRFYLRSLWHSMILGVATVIASSVLGITVAFLLVRYDFVGRSLFSYLTMIPI
                     ISPPLVGVLGFTFILGRAGTVNVLLMDLFDLLRPVNFLYGIHGVVLVETLHLFPMITL
                     NVVDALGKVDPSLEEAAESVGARGWSKLWRVTLPLTTPGYVAGALLVFIWTFSDFATP
                     LVLGVQDLLAPQAYLNIVQFVDRRIFRMGIVISALMVLLAVLFLLAARHYVAIKDYSS
                     LSYSRVARRRLTAGRQALVVAFLSFLLLLSFIPYIGVTLASVGKGWALTPLPVAYTLQ
                     YYERVIVET"
BASE COUNT          493 a         1206 c         1285 g          564 t
ORIGIN      
        1 acggcggcgc ggcggccaac gacttcctct gccagttcca ggccgacgtg ctgggggtgg
       61 aggtgctgcg tccctcggtg accgagacca ccgggctcgg cgcggcctac ctcgccgggg
      121 tcggcgcggg actctggaag agccgggacc tggccggccg ctggaagctc gagcgcgagt
      181 tcacccccgc gatgtcgccc gccgcgcgcg aggtgggtta cgcgggctgg cgccgcgcgg
      241 tggagcgctc gcgcggatgg atcgaacccg acaggaggga tgcatgatgc gatcgaccgt
      301 gacgagggcc gccctggccc tgattgccgc gctgagcgtg ctcgccccga tcggagcgcc
      361 ggcccgggcg caggcgccca tcgagggcga gctgaacctg atcaccccgg tctccaagtt
      421 catccacgac gccgcgctca aggccttcgc ggactacgcg aaggagaagt ggaacgtcac
      481 cgtgaaggtg agcgcgatcc cggccggcac cccggtggcg tacgggcgca tcaccgagtg
      541 gaagggcaag ccggaggtcg acatcttctg gggcggggag tcggcgctgt tcgagaagct
      601 ggccgagcag aagctcctgc agaaggtgga gatctcgaag gaggcgtggg agtccatccc
      661 ggccgcgatc ggcaagccca agccgatccc cctcaaggac cgggacggct actgggtcgg
      721 caccacgctc gagccgtacg ggatcgtcta caacccgaag cgcctgcagc gcctcggggt
      781 ggcgcccctc aaggactggg acgacctgct caatccgaag ctcaagggcg aggtggccca
      841 gtgcgccccg acccgctcgt cctcgtcgaa cgcgacgtac gaggtcatcc tctcgctgca
      901 gggcgaggag ccgggctggg gctggctgcg caagctcgcg gccaacaccg ggcacttcac
      961 cgcgcggagc cgggacgtgc cgacggtggt ggccaagggc gagttcgcga ccggcttcgc
     1021 ggtgccgtcc tacatggcgt tcgaggagaa gctggccggc ttcgacatca agtacgtggc
     1081 gcccaaaaac gccttcgtga ccccggagcc gatggcgatc ctggccgggg cccgcaaccc
     1141 cagggcggcg cgcgcgttca tcgagttcct gctgaccgag cgcgggcaga aggtgttcat
     1201 ggagcgcggg ctcttctcgg tcaacccgaa gtacaaggtg cagggcgcgc cgggctcgac
     1261 cgcggagatt gcggtggagt tcaccggcgg ggtgcggtcg tacttcgacg gcgacgtcgc
     1321 caacgtgtac gacgaggcgg tggcggccag gcggtcggag gcgctcaaga cccgcttccg
     1381 ctcggacatc gaggccaagt gggacgagct gaagaagtag cgcggctcgg cgccgcgccg
     1441 cgcgccgcga tcggatgatc gtcaccatcc agggggtggt gaagcgcttc ggtgtggtga
     1501 ccgcggtcga cgcggtggac ctgaccgtcg ccgacggcga gctgttcacg ctgctggggc
     1561 cgtcgggctg cggcaagacc accctcctgc ggctgctcgc cggcttctac cagccggacg
     1621 gcggcaccat ccgcttcggc gatcgggtcg tcaacgggct cgcgccctac gagcggcgca
     1681 tcggcatggt cttccagaac tacgcgctct ggccgcatat gacggtggcc gagaacatcg
     1741 gctacggcct tcgcctgcgc cgactgcccg gcgaggaggt cgcccggcgg ctgcaggaag
     1801 ggctgcgcaa ggtcaacctg accggcttcg aggcccgcta cccgggccag ctctcgggcg
     1861 gccagcagca gcgggtggcg ctcgcgcggg cgctggtgct gaacccggac atcctgctgc
     1921 tggacgagcc gctctcgaac ctggacgcca agatccgcat ccaggtgcgg gcggagatcc
     1981 ggcggctgca gcgggagctc gcgatcacca ccatctacgt gacccacgac caggaggagg
     2041 cgctgtcgct ctccgaccgg gtcgcggtca tgcgcgacgg gcgcgtgctg caggtcggtc
     2101 cgccccgcga cctctacgag cgcccgcgca cgcgcttcgt ggccgacttc gtggggacca
     2161 acaacctggt gccgggcgag gtggagagcc gggccgcgga gggcgtcgac atggtggtcc
     2221 gcaccgcgct cggcccgctg cgggccatcg ccaacggggc cgtctcggcg cgctgcgtgc
     2281 tcgcggtgcg gcccgagaac gtggcgctcg ggccggccgc aacgggcgag ggcaaccggg
     2341 tcgcggggcg cgtcagcctc gtctcctatc tcgggagcac ggtgcgctac gacgtggaga
     2401 ccgaggcggg cctggtgctg aaggccgaca tccgggacgc gtggcaccac gacccgctgc
     2461 cggtgggccg gccggtgacc ttgagcttcc cgtcgtcggt gaccctggcc gtcgcggacg
     2521 atgcgtagcc cgcgcctggc gctgggcgtc gggctcatct gggcgtttct gatcctcttc
     2581 ctggtctacc cgctcgggcg catcttctac gacgcggtga ccgacgaggc ggggcggtgg
     2641 acgctggcca acttccatga gttcttcacc gaccggttct acctgcgctc gctctggcac
     2701 tcgatgatcc tgggcgtggc gacggtgatc gcgtcctcgg tgctgggcat cacggtggcc
     2761 ttcctgctgg tccgctacga cttcgtcggc cgcagcctct tcagctacct caccatgatc
     2821 ccgatcatct cgcccccgct ggtgggggtg ctgggcttca ccttcatcct cgggcgcgcg
     2881 ggcaccgtga acgtgctcct gatggatctc ttcgacctcc tgcgaccggt gaacttcctc
     2941 tacggcatcc acggggtggt gctcgtcgag acgctccatc tcttcccgat gatcacgctc
     3001 aacgtggtgg acgcgctcgg caaggtcgac ccctcgctgg aggaggcggc ggagagcgtg
     3061 ggggcgcgcg ggtggagcaa gctctggcgg gtgaccctgc cgctcaccac cccgggctac
     3121 gtggcggggg cgctgctcgt gttcatctgg accttctccg atttcgcgac gccgctggtg
     3181 ctgggggtgc aggacctgct ggccccgcag gcctacctca acatcgtgca gttcgtggat
     3241 cggcgcatct tccgcatggg catcgtgatc tccgcgctga tggtgctgct cgcggtcctc
     3301 ttcctgctcg cggcgcggca ctacgtggcg atcaaggact actcgtcgct ctcctacagc
     3361 cgggtcgccc ggcggcgcct caccgcgggc cggcaggcgc tggtggtggc cttcctgtcc
     3421 ttcctgctgc tgctgtcctt catcccctac atcggggtga cgctcgcctc ggtgggcaag
     3481 ggctgggcgc tcaccccgct gccggtggcc tacacgctgc agtactacga gcgcgtgatc
     3541 gtggagac
//
LOCUS       DATMCV010000405         3805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_684607, whole genome shotgun sequence.
ACCESSION   DATMCV010000405 DATMCV010000000
VERSION     DATMCV010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684607"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VNU03_09400"
     CDS             <1..733
                     /locus_tag="VNU03_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP74721.1"
                     /translation="VGLENFTKLLADPDYYASVVNSFVFAGGVTVLAVLGSLVLAGLA
                     TQKIRGLAVYRTLLLWPYGIAPAVAGIIFLFIFHPAYGILPYFLSFVTDYQFNWLLKS
                     WVAMILVIVATAWTHLGYNIAFYIAGLLAIPASVIEAADVDGAGPIRRFTAIVFPLVS
                     PITFYLVVLNMVFAFFESFGVIDAVTKGGPGRSTEIMVYKLYRDGFIGLNLGSSGAQS
                     VILMAMVIALTVLQFRFAEKKVTYR"
     gene            899..1795
                     /gene="ugpE"
                     /locus_tag="VNU03_09405"
     CDS             899..1795
                     /gene="ugpE"
                     /locus_tag="VNU03_09405"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009816492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HWP74722.1"
                     /translation="MSAVGATQLEQAQVRARAADAPRRERAGGGALLVHALLIACIAV
                     VVFPLYFAFVVSTQDLKEVVQRPPRLLPSTHLVENYTEAWRKSGMGVMLFNSAVMAVT
                     VAVGKIIISMLSAFAVVYFSFRGRMVCFWMIFVTLMLPVPVRLVSTYEVIGTLGWLNS
                     YQGLTVPLMASATATFLFRQFYLTIPNEMAEAAQLDGAGPLRFLFRFLLPLSWANIAA
                     LFVVLFIFGWNQYLWPLMITNTEAMRTVVIGLEHLIPRSGTDLPTWNLIMAGAMMALL
                     PPVAIIVFMQRWFVKGLVESDK"
     gene            1792..2622
                     /locus_tag="VNU03_09410"
     CDS             1792..2622
                     /locus_tag="VNU03_09410"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWP74723.1"
                     /translation="MRGLLLWGLALGGFSAVLGTVGAAAPDMIAPLFAAHRGGAALWP
                     ENSLLAFGNALALGVDYLELDVHLSRDGEVMVIHDATLERTTTGSGTVRERTAAELGA
                     LRLKDRGGAVTAEPVPTLDQVVALAAAGKRQMLLEIKTDERRQRYPGIEEKVFAVLDR
                     QGYTPLAIVMSFEGETWRRLRQLRPETGTGALYSPRMLPASEIERELQSLRQAGVGFV
                     GLDQRLVSADVARQARLAGLTLGVWTVNEREAIERFIGHGVGIVITDRPDLAKAALGR
                     "
     gene            2619..>3805
                     /locus_tag="VNU03_09415"
     CDS             2619..>3805
                     /locus_tag="VNU03_09415"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HWP74724.1"
                     /translation="MTARRFVLALDQGTTGSTALVVDPDGLVRARGYAELPQHYPQPG
                     WVEHDPAQIWATTVQALGQAVAAAGIAPAEIAAVGITNQRETAVLWERASGRPVGNAI
                     VWQCRRTAALCDRLRADGAEPEIRRKTGLVVDAYFSGTKIRWMLDHQPALRPCAERGE
                     LCFGTVDSWLLWQLTGGAVHATDPSNASRTLCYDIHALGWDEGLCRILGVPMALLPAV
                     KPSAGVFGETTATAAPPLPGGIPVAGIAGDQQAALFGQACVEPGMAKNTYGTGCFALL
                     NTGTRAVGSSEGLLTTVAWTVEGATTYALEGAVFIAGAAVQWLRDGLGVIQQAAETQA
                     LAQSVPDTGGVYLVPAFVGLGAPYWDPYARGTLVGITRGTTRAHLARAALEAIAYQSR
                     DVL"
BASE COUNT          505 a         1353 c         1350 g          597 t
ORIGIN      
        1 cgtcggtctg gagaacttca ccaagctgct ggccgatccc gactactacg cgagcgtggt
       61 gaactcgttc gtgttcgcgg gcggggtgac cgtcctcgcg gtgctgggct cgctggtcct
      121 cgccggtctc gcgacccaga agatccgcgg gctcgcggtc taccgcaccc tgctgctgtg
      181 gccctacggc atcgcgcccg cggtggcggg catcatcttc ctgttcatct tccatcccgc
      241 ctacgggatc ctgccctact tcctctcctt cgtgaccgac taccagttca actggctgct
      301 caagagctgg gtggccatga tcctggtgat cgtggccacc gcgtggaccc acctcggcta
      361 caacatcgcg ttctacatcg cggggctcct cgcgatcccg gcgtcggtga tcgaggcggc
      421 ggacgtggac ggagccgggc cgatccggcg cttcaccgcc atcgtgttcc ccctcgtctc
      481 gccgatcacc ttctacctgg tcgtgctcaa catggtgttc gcgttcttcg aatccttcgg
      541 ggtcatcgac gcggtgacca agggcgggcc cggccggtcc accgagatca tggtctacaa
      601 gctctaccgc gacggcttca tcgggctcaa cctgggctcc tcgggcgccc agtcggtgat
      661 cctcatggcg atggtcatcg ccctcaccgt cctccagttc cgcttcgccg agaaaaaggt
      721 cacctaccga tgaggccagg gggcggcgag caggagcgcg ggttggccgg gcgccccgat
      781 gagcgccaaa ggcgcgaaga ggccctaccg ccccgcgatc tcctccaccg aagcggacta
      841 agaacaggaa gtcacgaatg accgcggatc gccggcggcc cggcacgaga ggtgaggaat
      901 gagcgcggtc ggcgcgaccc agctcgagca ggcccaggtc cgggcgcgcg cggccgacgc
      961 cccgcgacgg gagcgtgccg gcggaggcgc gctgctcgtc cacgcgctcc tgatcgcgtg
     1021 catcgcggtg gtggtgttcc cgctctactt cgcgttcgtc gtttccaccc aggacctgaa
     1081 ggaggtggtg cagcggccgc cgcggctcct gccgtccacc cacctggtgg agaactacac
     1141 cgaggcgtgg cgcaagtcgg gcatgggcgt catgctgttc aacagcgcgg tgatggcggt
     1201 gacggtggcg gtgggcaaga tcatcatctc gatgctgtcc gccttcgcgg tggtgtactt
     1261 cagcttccgc ggccgcatgg tctgcttctg gatgatcttc gtgaccctca tgctgccggt
     1321 gccggtgcgg ctcgtctcca cctacgaggt catcggcacc ctgggctggc tcaacagcta
     1381 ccaggggctg accgtgcccc tcatggcctc cgcgaccgcc accttcctgt tccggcagtt
     1441 ctacctgacc atcccgaacg agatggcgga ggcggcccag ctcgacgggg cgggcccgct
     1501 gcgcttcctg ttccggttcc tgctgccgct gtcgtgggcc aacatcgcgg cgctcttcgt
     1561 ggtgctgttc atcttcggct ggaaccagta cctgtggccg ctcatgatca ccaacaccga
     1621 ggcgatgcgc acggtggtga tcgggctcga gcacctcatc ccgcgcagcg gcaccgacct
     1681 gcccacctgg aacctcatca tggcgggggc catgatggcc ctgctgcccc cggtggccat
     1741 catcgtcttc atgcagcgct ggttcgtgaa gggcctggtg gagagcgaca agtgaggggg
     1801 ctcctgctct ggggcctggc cctgggcggc ttctccgcgg tgctcggcac cgtgggcgcg
     1861 gcggcgcccg acatgatcgc gccgctgttc gcggcccacc ggggcggggc cgccctctgg
     1921 ccggagaaca gcctgctcgc cttcggcaac gcgctcgcgc tgggcgtcga ctatctcgag
     1981 ctggacgtgc acctgagccg cgacggcgag gtgatggtga tccacgacgc caccctcgag
     2041 cgcaccacca ccgggagcgg gacggtgcgg gagcgcaccg cggccgagct cggggctctg
     2101 cggctcaagg accggggcgg ggcggtcacc gcggagccgg tgcccaccct ggaccaggtg
     2161 gtggccctcg ccgcggcggg caagcggcag atgctgctcg agatcaagac cgacgagcgg
     2221 cgccagcgct accccggcat cgaggagaag gtcttcgcgg tgctggaccg gcaggggtac
     2281 acgccgctcg cgatcgtgat gtcgttcgag ggggagacct ggcggcggct caggcagctc
     2341 cgcccggaga cgggcacggg agcgctctac tcgccgcgga tgctgcccgc gtcggagatc
     2401 gagcgggagc ttcagtcgct gcgccaggcc ggcgtcggct tcgtcgggct cgaccagcgc
     2461 ctggtgagcg ccgacgtggc gcggcaggcg cggctggccg ggctgaccct cggggtgtgg
     2521 acggtgaacg agcgtgaggc catcgagcgc ttcatcggcc acggggtcgg catcgtgatc
     2581 accgatcgcc ccgacctggc caaggcggcg ctcggccggt gaccgcgcgc cgcttcgtcc
     2641 tggcgctcga tcagggcacg accggctcga ccgcgctggt ggtggatccc gacggcctcg
     2701 tgcgggcccg gggctacgcg gagctgccgc agcactaccc gcagccgggc tgggtcgagc
     2761 acgatccggc ccagatctgg gccaccaccg tgcaggccct cggccaggcg gtggccgccg
     2821 ccggcatcgc gccggccgag atcgcggcgg tgggcatcac caaccagcgc gagaccgccg
     2881 tcctctggga gcgcgcctcc gggcggccgg tcggcaacgc gatcgtctgg cagtgccggc
     2941 gcaccgccgc gctctgcgac cgcctccgcg ccgacggggc cgagcccgag atccggcgga
     3001 agaccgggct ggtggtggac gcctacttct cgggcaccaa gatccgctgg atgctcgacc
     3061 accagccggc gctgcggccc tgcgctgagc gcggcgagct ctgctttggc accgtggact
     3121 cgtggctgct ctggcagctc accggcgggg cggtgcacgc gaccgatccg tccaacgcct
     3181 cgcgcacgct ctgctacgac atccacgcgc tcgggtggga cgagggactc tgccgcatcc
     3241 tcggcgtgcc gatggcgctc ctgcccgcgg tgaagccgtc ggccggcgtg ttcggggaga
     3301 ccacggcgac ggcggctccc ccgctgccgg gcggcatccc ggtggccggc atcgcgggcg
     3361 atcagcaggc cgccctcttc ggccaggcct gcgtcgagcc cggcatggcc aagaacacct
     3421 acggcaccgg gtgcttcgcg ctgctcaaca ccgggacccg cgcggtcggc tccagcgagg
     3481 gcctgctgac cacggtggcg tggacggtgg agggcgcgac cacctacgcg ctcgagggcg
     3541 cggtgttcat cgcgggcgcg gcggtccaat ggctgcggga cggcctgggc gtgatccagc
     3601 aggcggccga gacacaggcc ctggcccagt cggtgccgga caccggcggg gtctacctgg
     3661 tgcccgcttt cgtcggcctg ggcgcgccgt actgggaccc gtacgcgcgc ggcaccctgg
     3721 tgggcatcac gcgcggcacc acgcgcgccc acctggcccg cgccgcgctc gaggccatcg
     3781 cctaccagag ccgcgacgtg ctcga
//
LOCUS       DATMCV010000406         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_488848, whole genome shotgun sequence.
ACCESSION   DATMCV010000406 DATMCV010000000
VERSION     DATMCV010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488848"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2216)
                     /locus_tag="VNU03_09420"
     CDS             complement(<1..>2216)
                     /locus_tag="VNU03_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWP74725.1"
                     /translation="RLRASLAVASPLVLGISRDALVSGGKKIEASHVRAFARALYRRN
                     GGVLRFEEDVEPRELETFLAMLGDTGPARDRALTVEELRAAGIIHIHVSAIDYSALVT
                     TSDLDEPGGGDGSLWDGLLQTLLAGQPVFGGGMALPRERYSAETIASLFRGGPGAGGS
                     GPGGSESGGSPGPGGGPSGAGPGSGGPIGGGPGGGGPGGSGSAGGGWPGSGAGRGAGA
                     QASPPAGETGGRGGQGARLGLAAAHTLAAAVAARLASADDDQRESLAPQIIQLIRALP
                     GEVREPVLAAALRVLATEDAASAHLARLAAALPPADVLRGLRRLAQEQGQLSSHALRM
                     AQALAEAHEDLPPTPGLPEPPADFAAMAALFREEDVDRYNPEDHRALLAQKPTVDLAA
                     IAVELAADPDAFGPDTESDDAIERRVVTTLLDMTAVSPDVVRPLVLGRLRDIFVRALQ
                     ASRFAQAISMIRGIRDLAADPSQAERRRALDEFVEHLADAPTLAALVAASRQPGGPPF
                     VQVQSLVLALGASAAHGLLEALAAEPDRARRLRLIELAASLGPAIVPETRRLLADSRW
                     YVVRNMVLLLRRVHDKSAMSEILHCADHPDLRVRLEAIRALFAFDTKVPRDLLARTIN
                     HPDPRLAEAAVLLSGQHGITQATDLLVEILLRWDFLGRRRSLRLKALRALADLADPAV
                     LPRLDRFFRERPFPVVALEERRAAYRLLGSYDQAARAAYLARGEKSRDSLTRDICR"
BASE COUNT          274 a          816 c          880 g          246 t
ORIGIN      
        1 ccggcagatg tcgcgggtca ggctgtcgcg ggacttctcg ccccgtgcca ggtaggccgc
       61 gcgcgcggcc tggtcgtagg agccgagcag gcgataggcc gcgcgccgct cctcgagggc
      121 caccaccggg aacggccgct cgcggaagaa gcgatcgagc cgcgggagca ccgcggggtc
      181 ggccaggtcc gcgagcgcgc gcagggcctt gagccggaga gagcgcctcc ggccgaggaa
      241 gtcccagcgg agcaggatct cgacgaggag atcggtggcc tgggtgatcc cgtgctggcc
      301 ggagagcagc accgcggcct cggccaggcg cggatccggg tggttgatgg tgcgcgcgag
      361 caggtcccgc ggcaccttgg tgtcgaaggc gaagagcgcg cggatggcct cgagccgcac
      421 gcggaggtcc gggtgatcgg cgcagtgcag gatctcgctc atcgccgact tgtcgtgcac
      481 gcggcggagc aggagcacca tgttccgcac cacgtaccag cgggagtcgg cgaggaggcg
      541 ccgcgtctcg ggcacgatgg cggggccgag cgaggccgcc agctcgatca agcggagccg
      601 ccgcgcccgg tccggctcgg cggccagcgc ctcgaggagg ccgtgggccg ccgaggcgcc
      661 gagggccagc accaggctct gcacctggac gaagggcggg ccgcccggct gccggctcgc
      721 ggccaccagc gcggccaggg tcggcgcgtc ggccaggtgc tccacgaact cgtcgagcgc
      781 ccgccggcgc tccgcctggc tcgggtcggc ggccaggtcg cggatcccgc ggatcatcga
      841 gatggcctgg gcgaagcggc tcgcctggag cgcgcgcacg aagatgtcgc gcagacggcc
      901 gagcacgagg gggcggacca cgtcggggga caccgcggtc atgtcgagca gggtcgtcac
      961 cacgcggcgc tcgatcgcgt cgtcgctctc ggtgtccggg ccgaaggcgt ccgggtcggc
     1021 ggccagctcg accgcgatcg cggccaggtc caccgtcggc ttctgcgcga ggagggcgcg
     1081 gtgatcctcc gggttgtagc ggtcgacgtc ctcctcgcgg aagagcgcgg ccatcgccgc
     1141 gaagtcggcc ggcggctcgg ggaggcccgg ggtcggcggc agatcctcgt gcgcctcggc
     1201 cagcgcctgc gccatgcgca gcgcgtgact cgagagttgg ccctgctcct gcgccaggcg
     1261 gcggagcccg cgaagcacgt ccgcgggcgg cagcgcggcc gcgagcctcg ccaggtgcgc
     1321 gctcgccgcg tcctcggtcg cgaggacccg gagggcggcg gccagcactg gctcgcgcac
     1381 ctcgccgggc agggcccgga tcagctggat gatctgcggc gccagcgact cccgctggtc
     1441 gtcgtctgcg ctcgcgaggc gggccgcgac cgcggcggcg agggtgtgcg cggcggcgag
     1501 gccgagccgg gccccctggc cgccgcggcc acccgtctcg ccggccggcg gggaagcctg
     1561 ggcccccgcg ccgcgcccgg cgccgctgcc gggccagcca cccccggccg atccgctccc
     1621 acccggcccg ccacctcctg gccctccgcc gatcgggccg ccactccccg ggccggcccc
     1681 cgacggcccg ccgccggggc cgggcgagcc gccgctctcg gatccacccg ggccggaccc
     1741 tcccgcgccg gggccgccgc ggaagagcga ggcgatggtc tccgccgagt agcgctcgcg
     1801 ggggagggcc atgccacccc cgaacaccgg ctgaccggcg agcagcgtct ggaggagccc
     1861 atcccagagc gatccatcac cgccgccggg ctcgtccagg tcggaggtcg tgaccagcgc
     1921 gctgtagtcg atggcggaga cgtggatgtg gatgatgccg gccgcgcgca gctcctcgac
     1981 ggtcagggcc cggtcccggg cggggccggt gtcgcccagc attgcgagga aggtctccag
     2041 ctcgcgtggc tcgacgtcct cctcgaagcg gagcacgccg ccgttgcggc ggtagagcgc
     2101 ccgggcgaac gcccgcacgt gtgaggcctc gatcttcttg cctccgctca ccagggcgtc
     2161 gcgggagatc ccgaggacca gcgggctcgc gacggccagg gaggcgcgga gccgcg
//
LOCUS       DATMCV010000407         6553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_289923, whole genome shotgun sequence.
ACCESSION   DATMCV010000407 DATMCV010000000
VERSION     DATMCV010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289923"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VNU03_09425"
     CDS             <1..590
                     /locus_tag="VNU03_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP74726.1"
                     /translation="AFEELTPQRAREFILRNRRLLGAVRTAPFPTIAAINGHCLGTGL
                     GLALVADIRIAVPHATFGLPEIKVGVPSVCDVALLQQHIGLAKAKEVILTGDSYPVAE
                     LAPYGLLNAVVPAEQLVSETRRMLARVAGHTRAVTAGQKKLFEIWQNHPLTVSIDMSV
                     DVFAGVFAAPETYEQIERHRRDVGGKRRTDPGGLV"
     gene            623..1795
                     /locus_tag="VNU03_09430"
     CDS             623..1795
                     /locus_tag="VNU03_09430"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWP74727.1"
                     /translation="MEFAERMSRLGTESAFEVLARAKALERQGKEIIHLEIGEPDFDT
                     PKHIREAGKRALDSGATHYGPAAGLPELREAIVKDVEGTRSLPVTADEIVVTPGAKPI
                     MYFVITALVNPGDEVVHPNPGFPIYESVINFVGGKPVPIPLREESGFGFDLDVLEKSV
                     SRKTKLIIINSPQNPTGGVLEVDQLGRIAEIAKHYRIPVLADEIYKAFLYEGEFASIT
                     RFPGMKDLVIILDGFSKAYAMTGWRLGYGVMPVALAEHVTRLMVNSNSCTASFTQHAG
                     IAALQGDQTPVTEMVAEFKRRRDVLVAGLNRLPGVSCRSPRGAFYVFPNVKTLKRPST
                     EIAEALLQEAGVAVLGGSAFGQYGEGYLRLSYASSEANLNKALERMEPVLRRLAGG"
     gene            1844..3487
                     /locus_tag="VNU03_09435"
     CDS             1844..3487
                     /locus_tag="VNU03_09435"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWP74728.1"
                     /translation="MPTVADLLVEGLLRAEVPRLFGVPGGGSNLEVLEAARARGLPFV
                     LCHQEWAACIMAAVTGELTGHPGAVLSTLGPGVTASATGLAHARLDRSPLIYISDRHP
                     ASVLEYATHQYLDHTAHLGPITKGSVTVGADSAAHWIAHAVQLALAEPRGPVHLDLPA
                     DVAGRSAMPVATSVTPAPPAALDGDLVERAAQMIRAARRPLVIAGLGCRAADAKWLRA
                     FAEALPAPVLTTYKAKGAIPDPHPLALGIFTGGALEEPLVRRADLIIAFGLDTVELIP
                     RRWAYTAPVLNLTRCAAADARLRAPGGDAYFTPALEVVGEPGAILEDLASRIMGPGAR
                     ADWDVAEVDRIRRERLAALEVPVPGLAPHRVAQMTRELTAAGSIATVDAGAHMFQATT
                     YWQALEPGELLISNGLATMGFALPAAIAAQLVHPDRRVICFTGDGGLMMVASELETVA
                     RLRLPLLIVVFNDEALSLIEVKQEQKGFEGVSMRYAGPDLRALARAFGLRAFTATDEA
                     SLHTALIAAQTAPGPALIDARVDPSGYRRMLEIVRGAPK"
     gene            3507..3932
                     /locus_tag="VNU03_09440"
     CDS             3507..3932
                     /locus_tag="VNU03_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74729.1"
                     /translation="MAFLQARDEAAVKKEFERIGGPVKLTVFATELGGDSNAQAVALI
                     REVAALSDQLSVTVLNPHIDRDQAAAYGITESPAVVVEGARDYGIRFLGIPAGYEFSN
                     LIDSIIAVSTGEAGLTDETKAALAGLTEDVTIKVFATPT"
     gene            3945..4169
                     /locus_tag="VNU03_09445"
                     /pseudo
     CDS             3945..4169
                     /locus_tag="VNU03_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            4339..4740
                     /locus_tag="VNU03_09450"
     CDS             4339..4740
                     /locus_tag="VNU03_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HWP74730.1"
                     /translation="MAGDLLFTYGTLMRGFRLHRLLEGRAAYLGRGRVVGRLLDLGSY
                     PAAVADPGGTSAIHGELYRLRDPALWGTLDSAEGPQYHRREVTVRSEDGRDQAACIYW
                     YIGPLDRGVPIPGGDYRAHAPARSIYHQPSP"
     gene            4751..6106
                     /locus_tag="VNU03_09455"
     CDS             4751..6106
                     /locus_tag="VNU03_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWP74731.1"
                     /translation="MPVEAAQLIKDDQDHLIHPLHHPSDHLEPMVYVRGRGAVLTDVQ
                     GREYIDGLAGLWNVNVGHGRTELADAAANQIRELAYYSAYSGSTNIPAIQLASKLMTI
                     AYPNMQGVFFTSGGAESNESAFKTARFYWKTKGKPDKVKIIARSLAYHGVTLQAMSAT
                     GMSAYWKMFEPRVPGFVHIPTCYPYRQEGSKPGETAGQTAARLLEEAIVREGADTVAA
                     FIAEPIHGGGGVLYPTDDYFPLVRKVCDKHNVLFIADEVITGFCRTGKWFALEHWNVL
                     PDIVSFAKGVSSGYLPLGGIMVSKTIKQAMDEVKPEDKWMHAYTYSGHPTCCAVGIAN
                     LEIMERERLWERAAAMGTRLHKGLQQALGDHKHVGDIRGGKGLLACVELVEDRGTKAS
                     FGADKKVGPRVLAEMTKRGVITRARLEHIFFAPPLIVSEEQVDRLVSVTHDAIKAVTG
                     A"
     gene            complement(6169..>6553)
                     /locus_tag="VNU03_09460"
     CDS             complement(6169..>6553)
                     /locus_tag="VNU03_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP74732.1"
                     /translation="DLGSGDGRIVIAAARDRGARGVGFEIDPALVAQAVERARRLGLA
                     DRVTFRQDDLFQADLREATVVTLYLSPDLNRRLRPKLLSELRPGARVVSHSFDMGDWA
                     PTQRLQVTSNEGTHTLYLWVVPGKQ"
BASE COUNT          984 a         2316 c         2313 g          940 t
ORIGIN      
        1 cagcgttcga ggagctcacg ccgcagcggg cccgtgagtt catcctcagg aaccggcggc
       61 tcctgggcgc ggtccggacc gcgccgtttc cgacgatcgc ggcgatcaac gggcactgtc
      121 tcggcaccgg gctcgggctg gcgctggtcg cggacatccg gatcgcggtg ccgcacgcca
      181 ccttcgggct cccggagatc aaggtcgggg tgccgtcggt gtgcgacgtc gccctcctcc
      241 agcaacacat cgggctggcg aaggccaagg aggtgatcct caccggggac agctacccgg
      301 tcgccgagct ggccccctac ggattgctga acgcggtcgt cccggcggaa cagctcgtgt
      361 ccgagacccg gcgcatgctt gcgcgggtgg ccgggcacac gcgggcggtg acggcggggc
      421 agaagaagct gttcgagatc tggcagaacc atccgctgac cgtgagcatc gacatgagcg
      481 tggacgtgtt cgcgggagtg tttgccgcgc cggagacgta cgagcagatc gagcgccacc
      541 ggcgggacgt cggcggcaag cggaggacgg acccgggcgg gcttgtctga tgcgcggaag
      601 ctctgctacg atcggcgcca ccatggaatt cgcggagcgg atgtcccggc tgggcacgga
      661 atcggcgttc gaggtgctgg cgcgcgccaa ggccctcgag cggcagggca aggagatcat
      721 tcacctcgag atcggcgagc cggacttcga cacgccgaag cacatccgcg aggcgggcaa
      781 gcgggcgctc gacagcgggg ccacccacta cgggccggcc gcgggcctcc ccgagctgcg
      841 cgaggccatc gtgaaggacg tggagggcac gcggagcctc ccggtcaccg cggacgagat
      901 cgtggtgacg ccgggggcca agcccatcat gtacttcgtg atcaccgcgc tggtgaaccc
      961 aggcgacgag gtcgtccacc cgaacccggg cttcccgatc tacgaatcgg tgatcaactt
     1021 cgtgggcggc aagccggtgc cgatccccct gcgcgaggag agcggcttcg gcttcgacct
     1081 cgacgtgctc gagaagagcg tctcgcgcaa gaccaagctg atcatcatca actcacccca
     1141 gaaccccacc ggcggcgtgc tcgaggtcga ccagctcggc cgcatcgccg agatcgcgaa
     1201 gcactaccgg atcccggtgc tcgccgacga gatctacaag gcgttcctct acgagggcga
     1261 gttcgcctcc atcacccgct tcccggggat gaaggacctg gtcatcatcc tcgacggctt
     1321 ctccaaggcc tacgcgatga ccggctggcg actcggctac ggggtgatgc cggtggcgct
     1381 ggccgagcac gtgacccgcc tcatggtgaa ctccaactcc tgcaccgcgt cgttcaccca
     1441 gcacgcgggc atcgcggcgc tgcagggcga ccagacgccg gtgaccgaga tggtggccga
     1501 gttcaagcgc cggcgcgacg tgctggtggc gggcctcaac cggctgcccg gcgtctcctg
     1561 ccgctcgccg cgcggcgcct tctacgtctt ccccaacgtg aagacgctga agcggccctc
     1621 gaccgagatc gcggaggcgc tgctgcagga ggcgggcgtg gcggtgctcg gtggctccgc
     1681 gttcggccag tacggcgagg gctacctgcg cctgtcctac gccagctcgg aggccaacct
     1741 caacaaggcg ctcgagcgca tggagccggt gctccggcgc ttggcggggg gctagcgggc
     1801 gcctagtggt ccggtccgtg cgcgccgccg ccggaggcgc gtgatgccca ccgtcgccga
     1861 tctcctggtc gagggcctgc tgcgggccga ggtgccccgc ctcttcgggg ttccgggcgg
     1921 aggatccaac ctcgaggtcc tcgaggccgc gcgggcccgc gggctgccct tcgtgctctg
     1981 ccaccaggag tgggccgcct gcatcatggc tgcggtcacc ggcgagctga ccggccaccc
     2041 gggggcggtg ctctccacgc tcggaccggg ggtcaccgcc agcgccaccg ggctggctca
     2101 cgcgcgactc gatcgctcgc cgctgatcta catcagcgac cgacatccgg cgagcgtgct
     2161 cgagtacgcg acgcatcagt acctcgacca caccgcccac ctggggccga tcaccaaggg
     2221 cagtgtcacg gtcggcgcgg actcggccgc ccactggatc gcccacgcgg tgcagctcgc
     2281 gctcgcggag ccgcgggggc ccgtgcacct cgacctgccg gcggacgtcg cgggcaggag
     2341 cgcgatgccg gtggcgacgt cggtgacccc ggcccccccg gcagccctcg acggcgacct
     2401 ggtcgagcgt gcggcccaga tgatccgcgc ggcccggcgc ccgctcgtga tcgcgggcct
     2461 cggctgccgc gccgcggatg ccaagtggct gcgcgcgttc gcggaggcgc tgcccgcccc
     2521 ggtgctgacc acctacaagg ccaagggcgc gatcccggat ccgcacccgc tcgccctcgg
     2581 catcttcacc ggcggggcac tcgaggagcc gctggtcagg cgcgcggacc tgatcatcgc
     2641 cttcggcctc gacaccgtcg agctgatccc gcggcggtgg gcctacactg cgccggtgct
     2701 caacctgacc cgctgcgcgg ccgccgatgc gcggctgcgc gcgccgggcg gcgacgccta
     2761 cttcacgccc gcgctcgagg tggtggggga gccgggcgcg atcctggagg atctggcgtc
     2821 gcggatcatg gggccgggcg cgcgggcgga ctgggacgtg gccgaggtgg accggatccg
     2881 gcgggagcgc ctggccgccc tcgaggtccc ggtgccgggg ctggccccgc accgggtggc
     2941 ccagatgacc cgcgagctga ccgcggcggg cagcatcgcc accgtggacg cgggggccca
     3001 catgttccag gccaccacct actggcaggc cctcgagccc ggcgagctgc tgatctcgaa
     3061 cgggctcgcc accatgggct tcgcgctgcc cgcggccatc gcggcgcagc tcgtccaccc
     3121 cgaccgtcgg gtgatctgct tcaccgggga cggcgggctg atgatggtgg cctccgagct
     3181 cgagacggtg gcgcggctcc ggctgccgct gctcatcgtg gtcttcaacg acgaggcgct
     3241 ctcgctgatc gaggtcaagc aggagcagaa gggcttcgag ggcgtctcga tgcgctacgc
     3301 ggggcccgat ctccgcgcgc tggcccgcgc cttcggcctg cgcgcgttca ccgcgaccga
     3361 cgaggcctcg ctccataccg ccctgatcgc ggcgcagacc gcgccaggtc ccgcgctgat
     3421 cgacgcgcgc gtcgatccct ccggctatcg ccggatgctc gaaatcgtcc gcggggcgcc
     3481 gaagtaaagc ccgtatactt cgagccatgg catttctcca ggcgcgagac gaagcggcgg
     3541 tgaagaagga gttcgagcgg atcggcggcc cggtgaagct caccgtgttc gccaccgaac
     3601 tgggcggcga cagcaacgcc caggcggtgg ccctgatccg ggaggtggcc gcgctgtccg
     3661 accagctgtc ggtcaccgtg ctgaaccccc acatcgatcg cgaccaggcg gcggcctacg
     3721 ggatcaccga gtcgcccgcg gtggtggtgg agggggcgcg ggactacggg atccggttcc
     3781 tgggcatccc ggccgggtac gagttctcca acctgatcga ttccatcatc gcggtgtcca
     3841 ccggagaagc cgggttgacc gacgagacca aggccgccct ggccgggctc accgaggacg
     3901 tcacgatcaa ggtcttcgcg accccgactt gaccgcactg cccccgggcc gtgcgcctgg
     3961 cgcagcacat ggcgatcgcg tccccccggg ttcgggccga gtgcatcgag gccaacgagt
     4021 tcccggagct gtcccagcgc taccaggtga tggcggtccc caagattgtc atcaacgatc
     4081 gcgtcggctt cgagggcgcg atccccgagc gggacttcct gggcgcggtc ctgcaggcgg
     4141 tgagcggcga agagcaggcg gagtcctgat cgatggaagc cgcgctcgcc gtcgtcgccc
     4201 tcctgatcgg ctggttcgcg ctcaatcgct gggtgctgcc gcgtctcggc atccagacct
     4261 gagccgctcc cggagcctgc tcccgtgagg agcaggaggc gaaggacccg caaccccgcg
     4321 cgtagcgggc gccccgcggt ggccggcgat ctcctcttca cctacggcac gctgatgcgg
     4381 ggcttccgcc tgcatcggct cctcgagggc cgcgcggcct acctggggcg cgggcgggtg
     4441 gtcggacgcc tcctggacct cgggtcgtac cccgccgcgg tggccgatcc cggcggcacg
     4501 agcgcgatcc acggagagct ctaccgcctc cgcgatcccg cgctgtgggg gacgctggac
     4561 tcggcggagg gaccccagta tcatcgccgg gaggtcaccg tccggagcga ggacggccgc
     4621 gatcaggccg cgtgcatcta ctggtacatc ggcccgctcg accgcggcgt gccgataccg
     4681 ggcggcgact accgggccca cgcgcccgcg aggtccatct accaccagcc gagcccgtag
     4741 gaggagtccg atgcccgtcg aggccgcgca gctcatcaag gacgaccagg atcacctgat
     4801 ccacccgctg catcacccga gcgaccatct ggagccgatg gtctacgtgc gcggccgcgg
     4861 cgcggtgctg accgacgtgc agggccgcga gtacatcgac gggctcgccg ggttgtggaa
     4921 cgtcaacgtg ggccacggcc ggaccgagct ggcggacgcg gcggccaacc agatccgcga
     4981 gctggcgtac tactcggcct attccggctc gaccaatatc ccggccatcc agctcgcgtc
     5041 caagctgatg accatcgcgt acccgaacat gcagggggtc ttcttcacct cggggggcgc
     5101 ggagtccaac gagtccgcgt tcaagaccgc ccgcttctac tggaagacca agggcaagcc
     5161 cgacaaggtg aagatcatcg cgcgctcgct cgcctatcac ggcgtgacgc tgcaggcgat
     5221 gagcgcgacc ggcatgtcgg cctactggaa gatgttcgag ccgcgggtgc cgggcttcgt
     5281 acacatcccg acctgctatc cgtatcggca ggaagggagc aagcccggag agaccgccgg
     5341 ccagaccgcg gcgcggctcc tcgaggaggc catcgtgcgc gagggcgccg acacggtcgc
     5401 cgccttcatc gccgagccga tccacggcgg gggcggcgtg ctctatccca ccgacgacta
     5461 cttcccgctg gtgcggaagg tctgcgacaa gcacaacgtg ctcttcatcg ccgacgaggt
     5521 catcaccggc ttctgccgca ccggcaagtg gttcgcgctc gagcactgga acgtgctgcc
     5581 cgacatcgtc tccttcgcca agggcgtctc gtcgggttat ctgccgctcg gcggcatcat
     5641 ggtctcgaag acgatcaagc aggccatgga cgaggtgaag cccgaggaca agtggatgca
     5701 cgcctacacc tactcggggc atccgacgtg ctgcgcggtc gggatcgcga acctcgagat
     5761 catggagcgc gagcggctct gggagcgcgc ggcggccatg ggcacgcgcc tgcacaaggg
     5821 gctccagcag gcgctgggcg atcacaagca tgtgggtgac atccgcgggg gcaagggcct
     5881 gctggcctgc gtcgaactgg tggaggaccg cgggaccaag gcctcgttcg gggccgacaa
     5941 gaaagtcggg ccgcgcgtgc tggccgagat gaccaagcgc ggcgtcatca cccgcgcccg
     6001 cctcgagcac atcttcttcg ccccgccgct gatcgtcagc gaggagcagg tcgaccgcct
     6061 cgtctcggtc acccacgacg cgatcaaggc cgtcaccggc gcctaagcgc gtctcatcct
     6121 ctcctccctc tcccccttca agggggagag ggcagggtga ggggagttct actgtttccc
     6181 cggcaccacc cacaggtaca gcgtgtgggt cccctcgttg ctcgtcacct ggagcctctg
     6241 cgtgggcgcc cagtccccca tgtcgaagct gtgcgagacg acgcgcgccc ccggccggag
     6301 ctcgctcagc agcttgggac gcagtcgccg gttgaggtcg ggggagaggt agagcgtgac
     6361 gacggtcgcc tctcgcaggt cggcctggaa gaggtcgtcc tgccggaacg tgacccgatc
     6421 ggcgagcccg agccgccgcg cccgctcgac ggcctgcgcc accagcgctg gatcgatctc
     6481 gaagcccacg ccgcgagctc cacggtcgcg ggcggcggcg atgacgatcc gcccatcgcc
     6541 agagccgaga tcg
//
LOCUS       DATMCV010000408         2968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_888587, whole genome shotgun sequence.
ACCESSION   DATMCV010000408 DATMCV010000000
VERSION     DATMCV010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888587"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..508
                     /locus_tag="VNU03_09465"
     CDS             119..508
                     /locus_tag="VNU03_09465"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWP74733.1"
                     /translation="MRRDDERTWTTRVLLGEIGRQLVRLAEEEVDLARAELASDARSA
                     GWTLVGFGLALVAGLVGLTLLLVAVVLALGQVMPAWLAALVVAAGVLAAGGLAGWLGW
                     VYRPRAALALTRKSLKEDWNWLKDLVA"
     gene            505..960
                     /locus_tag="VNU03_09470"
     CDS             505..960
                     /locus_tag="VNU03_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74734.1"
                     /translation="MIVTAAGRPRAPRAIEEEIRRRRAELGALAGELHRRGRELTNPR
                     LLIRRHAFGVTVALLAVGAATAGSIAYRARRARREGTLLARGGRLRDALGRMIDRPER
                     VAVEPTATQRIIGSAGSAAAAFLIKAALEHLSRPRQEARPRGRFARPSR"
     gene            complement(950..1669)
                     /locus_tag="VNU03_09475"
     CDS             complement(950..1669)
                     /locus_tag="VNU03_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74735.1"
                     /translation="MATEQTNGQEDDGGMGRLLRERLTRHPAPPMLRATVREALEPRT
                     ARQPWWMLWGAPAATAIATALVMLLWIAPSLPSAPATDPAQLVVRAVLADHARNIFWG
                     ESRTDVVPGALPLAMAESGVALNWVFTGDEDIRLINAKATYMEGRRGIELAYQDSAGH
                     TVTYVIVPGGSVTLPERGRVQIDRWRPVVRKENGFSLIIWRQQSLLCVLASDLVSDED
                     LGRFKQYFVKVRSSTDPYVTY"
     gene            complement(1686..2192)
                     /locus_tag="VNU03_09480"
     CDS             complement(1686..2192)
                     /locus_tag="VNU03_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP74736.1"
                     /translation="MADDEFRQQALQHLDALHNLAVYLTRNGSEAEDLVQETYVRAMR
                     FSHRFQPGTHLRAWLFQILRNTFLTFYRLRERESALSEDGVPESEAPMFHDAPSRDSE
                     SAGAHMDLDRALTKLPEEFKTPLLLAEVEGLALEDVARIMDCPVGTVKSRIFRAKERL
                     RGFLRDYE"
     gene            complement(2213..2755)
                     /gene="folK"
                     /locus_tag="VNU03_09485"
     CDS             complement(2213..2755)
                     /gene="folK"
                     /locus_tag="VNU03_09485"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWP74737.1"
                     /translation="MARVFLSVGSNLGDRLESLRRAVDQLRAMSDLRFLDASPLYRTE
                     PWEGAPGRKAEEWFFNCAIVIETELPPAALLARLQEVEQALGRVRRPGTPEAQRYDPR
                     PLDIDILLYDDRVISGPEHLHIPHLLMHERRFVLRPLADLAPDLEHPVLYQTIQELLE
                     VLEDEHEVLMSDLPRRWFED"
     gene            complement(2762..>2968)
                     /locus_tag="VNU03_09490"
     CDS             complement(2762..>2968)
                     /locus_tag="VNU03_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWP74738.1"
                     /translation="DYGVIAARIVEVSTKERVNLLERLAGRLAQMLLAEFPCDEVRVR
                     VRKLTPPMEGLQGIPGVELTRRRR"
BASE COUNT          410 a         1009 c         1108 g          441 t
ORIGIN      
        1 tggaacggct gagggcagag ctcgtcaagg ccagggaagc gctctccggg ggcatcctgg
       61 accggctcgt cgggctggtc cggccctgaa gcgggattcg ggtcagccgg aggagccgat
      121 gcggcgcgac gacgagcgga cgtggactac ccgggtgctg ctgggtgaga tcggccgtca
      181 gctcgtgcgg ctggccgagg aagaggtgga cctggcgcgc gccgagctgg cgagcgatgc
      241 gcggtccgcg ggctggaccc tcgtcggatt cggccttgcg ctcgtggcgg ggctcgtcgg
      301 actgaccttg ctcctcgtgg ccgtcgtgct cgcgctcggt caggtgatgc cggcctggct
      361 ggccgcgctc gtcgtggccg ccggcgtgct cgcggccggc gggctggccg gctggctggg
      421 gtgggtgtat cgcccgcggg cggccctggc cctcacccgg aaatcgctga aggaggactg
      481 gaattggctg aaggacctgg tggcgtgatc gtgaccgccg cggggcggcc ccgggccccg
      541 cgggcgatcg aggaagagat ccggcgccga cgggccgagc tcggcgcgct ggcgggggaa
      601 ctgcaccgcc gcggacggga gctgaccaac ccgagactgc tgatccgccg acacgccttc
      661 ggggtcacgg tggcgctcct ggcggtgggt gccgccaccg ccggctcgat cgcctatcgc
      721 gcccggcggg cccgacgcga ggggacgctc ctggcccgag gcgggcgcct gcgggacgcg
      781 ctgggccgga tgatcgaccg gccggagcgc gtcgcggtcg agccgaccgc gacgcagcgg
      841 atcatcggct cggccggctc ggcggcggcc gcgttcctga tcaaggcggc cctggagcac
      901 ctgagccgtc cacgtcagga ggctcgaccc cgcggccggt tcgcccggcc tagtaggtga
      961 cgtacgggtc ggtcgaggag cggaccttca cgaagtactg cttgaagcgc ccgaggtcct
     1021 cgtcggagac caggtccgac gcgagcacgc agagcaggct ctgctggcgc cagatgatga
     1081 ggctgaagcc gttttccttg cgcaccaccg gcctccagcg gtcgatctgc acccgcccgc
     1141 gctcgggcag ggtcacgctg ccgcccggca cgatcacgta ggtcacggtg tggccggccg
     1201 agtcctggta ggcgagctcg atgccgcggc gaccttccat gtaagtcgcc ttggcgttga
     1261 tgagccggat gtcctcgtcg ccggtgaaga cccagttgag cgcgaccccg ctctccgcca
     1321 tcgccagggg cagggccccg ggcaccacgt cggtccgcga ctcgccccag aagatgttgc
     1381 gggcgtgatc cgcgagcacc gcgcggacga ccagctgggc cgggtcggtc gcgggggccg
     1441 acggcaggct cggcgcgatc cagaggagca tgacgagggc ggtagcgatc gcggtcgcgg
     1501 cgggggcgcc ccagagcatc caccagggct ggcgcgcggt ccgcggctcg agagcctccc
     1561 gcaccgtcgc gcgcagcatg ggcggcgccg ggtggcgcgt cagccgctcg cgcagcagcc
     1621 ggcccattcc gccgtcgtcc tcctgtccgt tcgtctgttc ggtggccatg tcgttcgtga
     1681 ctcgactact cgtagtccct caggaacccg cgcagccgct ccttggcgcg gaagatccgg
     1741 gatttcacgg tgcccacggg gcaatccatg atgcgcgcca cgtcctccag ggccagcccc
     1801 tcgacctcgg ccagcaggag cggcgtcttg aactcctcgg gcagcttggt cagggcccgg
     1861 tccagatcca tatgggcccc ggcgctctca ctatcccggc tgggggcgtc gtgaaacatg
     1921 ggcgcctcgc tttccgggac tccatcctcc gagagggccg attcccgctc ccggaggcgg
     1981 tagaacgtca ggaacgtgtt tctcagtatt tggaacaacc aggcgcgcag gtgggttccc
     2041 ggctggaacc gatgggagaa ccgcatggcc cggacatagg tctcctggac caggtcctcg
     2101 gcctcggagc cgttccgggt cagatagacg gccagattgt gcagggcgtc caggtgctgg
     2161 agggcctgct ggcggaactc gtcgtcagcc acggaacact gccgggatca cgctagtctt
     2221 cgaaccagcg gcggggcagg tccgacatca gcacctcgtg ctcgtcctcc aggacctcca
     2281 ggagctcctg gatggtttga tagagcaccg ggtgctccag gtcgggagcc aggtcggcca
     2341 ggggccgcag cacgaaccgg cgctcgtgca tgagcaggtg ggggatgtgc aggtgctcgg
     2401 gaccgctgat cacccggtcg tcgtagagca ggatgtcgat gtcgagcgga cggggatcgt
     2461 agcgctgcgc ctccggggtg cccgggcgcc ggacgcgccc cagggcctgc tccacctcct
     2521 gcaggcgggc cagcagggcc gcggggggca gctcggtctc gatcacgatc gcgcagttga
     2581 agaaccactc ctccgccttg cggcccggcg cgccctccca gggctcggtg cggtagaggg
     2641 gcgaggcgtc gaggaaccgc aggtcgctca tggcgcgcag ctggtcgacc gcgcggcgga
     2701 gagactccag ccggtcgccc aggttcgatc cgacggacag gaacacgcgc gccatggctc
     2761 tctaccgccg ccgacgggtg agctccacgc cggggatgcc ctgcagccct tccatcgggg
     2821 gcgtgagctt gcggacccgt acccgcactt cgtcgcaggg gaactcggcg agcagcatct
     2881 gcgcgagccg tccggcgagg cgctccagca ggttcacccg ctccttcgtc gagacctcca
     2941 cgatgcgcgc ggcgatcact ccgtagtc
//
LOCUS       DATMCV010000409         5162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_757066, whole genome shotgun sequence.
ACCESSION   DATMCV010000409 DATMCV010000000
VERSION     DATMCV010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757066"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VNU03_09495"
     CDS             <1..1484
                     /locus_tag="VNU03_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP74739.1"
                     /translation="KRIAEKMNLSLTDADVASFLGLMKPSIDGYNVVDQLPDNLPPVK
                     YPRTPGYRPGPAENKHNAWYYKSKIEGAPQGKLKGKRVVIKDNVMVAGVPMMNGASTL
                     EGYTPEVDATVVTRILDAGGTILGKAHCEYYCLSGGSHTNATGPVHNPYKMGYSAGGS
                     SSGSAVLVALGEADMAIGGDQGGSIRMPASFCGIYGMKATHGLVPYTGIMPIEIYVDH
                     TGPMTATVADNALLLEAIAGPDGYDPRQFAPKVHPYTQLLGGGAAGLRIGVVKEGFGH
                     PQSEAVVDAKVRQAADLFKRLGATVTDISIPEHLLAGALWLPIGVEGLTQTMMWGDGY
                     GVSRQDLYVTSLMDHQRGWRQRANEMSETTKLFTMLGTYINEQHGSRYYGKAMNITRR
                     LTAAYDAALADCDLLLMPTTPMKAQAMPGPGASREEYVGGALNMITNTAPFDISHHPA
                     MAIPCGMADGLPVSVMLAGKHWDEPTIYRAAHAFEQAGDWKAM"
     gene            1519..2727
                     /locus_tag="VNU03_09500"
     CDS             1519..2727
                     /locus_tag="VNU03_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWP74740.1"
                     /translation="MTRRVLVTAITLIALVTMLTGLPGGLTAQGKVVKIGLSLPLTGA
                     DADGADAILKGAQMAIAEINAKGGAGGYKLEAMVFDSATPAAGQYDPAQAATNYKKFI
                     ADALVLAAVGPNMSGEGKAISPILSEADMATITPSSTNPDITDPKFQSQYRPKGKAVY
                     FRTVTTDAYQGPNMANYLFHAQKMKSVFVLDDGGAFGVGIADSFQKRATDLGVKILGR
                     DRLDPKEADYKTILTKIKGLNPDALYYGGVMQAAAKLATQGFEVMPRVIKAGSDGVYD
                     LSWPKQSGKESVEGWFCSQASPELVTSQKLEAWVTKFKNEMKREPTNYAITSYNAVLV
                     IADAVGRTVSANKPITRSSLRDAIQATKLSSIQGPIAFDANGDILTRTVSIYQWRDGE
                     IKYVGVAPEK"
     gene            2797..3723
                     /locus_tag="VNU03_09505"
     CDS             2797..3723
                     /locus_tag="VNU03_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74741.1"
                     /translation="MMDVVLQQVVNGLALGMMYALLALGFTMVYGIIELINFSHFSVF
                     MTGTFVGLTVLNLMGVTAVSRALTGWTLVATLIVLFLVTMVITGLLGMAIERLCLRPL
                     RNVSGTAPMITTIGVAFVLINVMLLTFGPQAQRFPSLLPNWRWPLYGTAEITFKQLVL
                     FAVGLVLMVILSHLVKRTTLGKAMRATAQDADAARMMGIDVDRVVVLTFFLGSALAGA
                     GSLFFGLYYGFTGYYIGYTTGLRAFTSAVLGGIGSIPGAVLGGLCIGLTQSLGGQLLG
                     VKWTDVMIFSILIFVMVLRPSGLLGVRAPQKA"
     gene            3720..>5162
                     /locus_tag="VNU03_09510"
     CDS             3720..>5162
                     /locus_tag="VNU03_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP74742.1"
                     /translation="MIARLRRLGARLGMERWRALPLSRRRQILTPALLGLGLLFPVLS
                     GNAGNVDAAANAFTYVLLALGLNIVVGFAGLLDLGYAAFFAIGAYGYGIAASGQLKPE
                     WSSFWAPLEWAGQVSRLVIPGQPDTVQFHVAFWPMLAVAAIICAIFGMLFGAPTLRLR
                     GDYLAIVTLGFGEIVPVVARNWEGLTNGAQGLGGIRTPRWFGYDFGFSPYPYYFLGLA
                     LVAAAVWISIRLQESRVGRAWMAIREDELAAGAMGVNHVHYKLLAFAMGAAVGGLGGT
                     FYVAKLTTATPDMFTFPVSVMLLVMVVLGGLGSIRGVVLAALFVSFLQSVILQDLTEH
                     IHALGRLVGSAFLQRVELITSLELIFGVILVVMMIFRREGLWPAVRRVSALTREQQTA
                     APTRGSVVELAWARPEAAGGAGRPLLEIEGLRKSFGGVAAADDIALTVRAGELVSVIG
                     PNGSGKTTLFNLITGLIAPDGGSIRLEGREI"
BASE COUNT          795 a         1814 c         1758 g          795 t
ORIGIN      
        1 tcaagcgcat cgccgagaag atgaacctct cgctcaccga cgccgacgtg gcgtcgttcc
       61 tcggcctgat gaagccgagc atcgacggct acaacgtcgt cgatcagctc ccggacaacc
      121 tgccgccggt gaagtacccg cgcacccccg gctaccggcc cgggcccgcg gagaacaagc
      181 acaacgcctg gtactacaag tcgaagatcg agggcgcgcc ccagggcaag ctcaagggca
      241 agcgcgtcgt gatcaaggac aacgtgatgg tggccggcgt gccgatgatg aacggggcct
      301 cgactctcga aggctacacc ccggaggtgg acgcgacggt ggtgacccgc atcctggacg
      361 cgggcggcac catcctcggc aaggcccact gcgagtacta ctgcctctcg ggcggcagtc
      421 acaccaacgc caccggcccg gtgcacaacc cgtacaagat gggctactcg gcgggcggct
      481 cgtcgtcggg cagcgcggtg ctggtggcgc tgggcgaggc cgacatggcg atcggcggtg
      541 accagggcgg ctcgatccgc atgcccgcct cgttctgcgg catctacggg atgaaggcga
      601 cacacgggct cgtgccctac accggcatca tgccgatcga gatctacgtg gatcacaccg
      661 gcccgatgac cgccaccgtg gccgacaacg cgctgctcct cgaggcgatc gcggggccgg
      721 acggctacga cccgcggcag ttcgcgccga aggtccaccc ctacacccag ctcctgggcg
      781 gcggggcggc cgggctccgc atcggggtgg tgaaggaggg cttcgggcat ccgcagtcgg
      841 aggcggtggt cgacgccaag gtgcggcagg ccgccgatct cttcaagcgc ctcggggcca
      901 ccgtcaccga tatctcgatc ccggagcacc tgctcgcggg cgccctctgg ctgccgatcg
      961 gagtcgaggg tctcacccag accatgatgt ggggcgacgg ctacggggtc agccggcagg
     1021 acctctacgt gaccagcctg atggaccacc agcgtggctg gcgccagcgg gccaacgaga
     1081 tgtccgagac caccaagctc ttcacgatgc tcggcaccta catcaacgag cagcatggca
     1141 gccgctacta cggcaaggcc atgaacatca cccggcggct caccgcggcc tacgatgcgg
     1201 cgctggccga ctgcgacctc ctgctgatgc cgacgacccc gatgaaggcc caggcgatgc
     1261 cggggccggg ggcgtcccgg gaggaatacg tcgggggcgc gctcaacatg atcaccaaca
     1321 ccgcgccctt cgacatcagc caccatccgg cgatggcgat cccgtgtggc atggccgacg
     1381 ggctgccggt cagcgtgatg ctggcgggca agcactggga cgagccgacg atctaccgcg
     1441 cggcgcatgc cttcgagcag gccggcgact ggaaggcgat gtaatcaggc cctacaccat
     1501 cgctcgggag gagacaccat gactcgacgt gtgctcgtga cggcaatcac cctcatcgcc
     1561 ctggtgacga tgctcaccgg gctgcccgga ggcctgaccg cgcagggcaa ggtcgtgaag
     1621 atcgggctct cgctgccgct gaccggggcg gacgccgacg gcgcggacgc gatcctcaag
     1681 ggcgcccaga tggcgatcgc cgagatcaac gccaagggcg gcgcgggggg ctacaagctc
     1741 gaggcgatgg tcttcgacag cgccaccccg gcggcgggcc agtacgatcc ggcccaggcg
     1801 gccaccaact acaagaagtt catcgcggac gccctcgtgc tcgccgcggt cgggcccaac
     1861 atgagcgggg agggcaaggc gatctcgccg atcctgtccg aggccgacat ggccacgatc
     1921 acccccagct ccaccaaccc cgacatcacc gatcccaagt tccagtccca gtaccggccc
     1981 aagggcaagg cggtgtactt ccgcaccgtg accaccgacg cctaccaggg cccgaacatg
     2041 gccaactacc tcttccacgc ccagaagatg aagagcgtct tcgtgctcga cgacggcggg
     2101 gccttcgggg tgggcatcgc ggacagcttc cagaaacggg ccaccgatct cggagtgaag
     2161 atcctcggcc gcgaccgcct cgatcccaag gaagcggact acaagaccat cctcaccaag
     2221 atcaagggcc tgaacccgga cgcgctctac tacggcgggg tcatgcaggc cgcggccaag
     2281 ctcgcgaccc agggcttcga ggtgatgccc cgggtgatca aggcgggctc ggacggggtc
     2341 tacgacctct cgtggccgaa gcagtcgggc aaggagtcgg tcgagggctg gttctgctcg
     2401 caggcctccc cggagctggt gacctcccag aagctcgagg cgtgggtgac caagttcaag
     2461 aacgagatga agcgcgagcc caccaactac gcgatcacgt cctacaacgc ggtgctggtg
     2521 atcgcggacg cggtcggacg caccgtctcc gccaacaagc cgatcacccg cagcagcctg
     2581 cgggacgcga tccaggccac caagctgtcc tcgatccagg ggccgatcgc cttcgacgcc
     2641 aacggcgaca tcctgacccg gaccgtctcg atctaccagt ggcgggacgg cgagatcaag
     2701 tacgtcggcg tggcgcccga gaagtaggcg gaagcccgcg ggagagagcc ccgggcccgg
     2761 cgccgtcgcg ccggcccggg gcgcctctcg cccgacatga tggacgtcgt cctgcagcag
     2821 gtggtcaacg ggctcgcgct cggcatgatg tacgccctgc tcgcgctcgg cttcaccatg
     2881 gtctacggca tcatcgagct gatcaacttc tcccacttca gcgtcttcat gaccgggacc
     2941 ttcgtggggc tcaccgtcct gaacctcatg ggcgtcaccg cggtgtcccg ggcgctgacc
     3001 ggctggaccc tggtggccac cctgatcgtc ctcttcctcg tcaccatggt catcaccggc
     3061 ctgctcggga tggcgatcga gcggctctgt ctccgcccgc tgcgcaacgt ctccgggacc
     3121 gcgccgatga tcaccaccat cggggtcgcc ttcgttctca tcaacgtcat gctgctgacc
     3181 ttcgggccgc aggcccagcg cttcccgtcg ctcctgccga actggcggtg gccgctctac
     3241 ggcaccgccg agatcacctt caagcagctc gtgctgttcg cggtggggct cgtgctcatg
     3301 gtgatcctct cccacctggt caagcggacc acgctcggca aggcgatgcg cgccaccgcg
     3361 caggacgcgg acgcggcccg catgatgggc atcgacgtgg accgggtggt ggtgctcacg
     3421 ttcttcctgg gctcggcgct ggccggggcg gggagcctct tcttcggcct ctactacggc
     3481 ttcaccggct actacatcgg ctacaccacc gggctgcgcg ccttcacctc ggcggtgctc
     3541 ggaggcatcg ggagcatccc gggcgcggtg ctgggcggcc tctgcatcgg gctcacccag
     3601 tccctcgggg gccagctcct gggcgtgaag tggaccgacg tgatgatctt ctcgatcctg
     3661 atcttcgtga tggtcctccg cccgagcggc ctgctcgggg tgcgcgcgcc gcagaaggca
     3721 tgatcgcgcg gctgcggcgg ctcggcgcgc gcctggggat ggagcgctgg cgcgcgctgc
     3781 cgctctcccg gcggcggcag atcctcaccc ccgcgctgct cggcctcggg ctgctgttcc
     3841 cggtcctgag cggcaacgcg ggcaacgtgg acgcggcggc caacgccttc acctacgtcc
     3901 tgctcgccct cggcctcaac atcgtggtcg gcttcgcggg cctgctcgac ctggggtacg
     3961 ccgcgttctt cgccatcggc gcctacggct acgggatcgc cgcctccggc cagctcaagc
     4021 ccgagtggtc gagcttctgg gcgccgctcg agtgggccgg gcaggtgagc cggctcgtga
     4081 tccccggcca gccggacacc gtgcagttcc acgtcgcgtt ctggcccatg ctggcggtgg
     4141 ccgcgatcat ctgcgcgatc ttcggcatgc tcttcggcgc gcccaccctg cggctgcgcg
     4201 gcgactacct cgcgatcgtc accctcggct tcggcgagat cgtgccggtg gtggcgcgca
     4261 actgggaagg gctcaccaac ggggcccagg ggctgggcgg catccggacc ccgcgctggt
     4321 tcggctacga cttcggcttc tcgccgtacc cctactactt cctggggctg gcgctggtgg
     4381 ccgccgcggt gtggatctcg atccggctgc aggagtcgcg ggtggggcgg gcctggatgg
     4441 ccatccgcga ggacgagctg gccgcggggg cgatgggggt caaccacgtc cactacaagc
     4501 tgctcgcctt cgcgatgggc gccgcggtgg gcgggctcgg cggcaccttc tacgtggcca
     4561 agctcaccac cgccaccccg gacatgttca cgttcccggt gtcggtgatg ctgctggtca
     4621 tggtggtgct gggcggcctc gggtccattc gcggggtggt gctggccgcc ctcttcgtgt
     4681 ccttcctcca gtcggtgatc ctgcaggacc tcaccgagca catccacgcc ctcgggcggc
     4741 tcgtcgggag cgcctttctc cagcgggtcg agctgatcac ctccctcgag ctgatcttcg
     4801 gggtcatcct ggtcgtgatg atgatcttcc ggcgcgaggg cctgtggccg gcggtccggc
     4861 gggtgtcggc gctgacgcgc gagcagcaga ccgcggcgcc cacccgcggc tccgtcgtgg
     4921 agctggcgtg ggcgcgcccg gaggcggcgg gcggggcggg ccggcccctg ctcgagatcg
     4981 aggggctgcg caagagcttc ggcggggtcg cggcggccga cgacatcgcc ctgaccgttc
     5041 gcgccggcga gctggtcagc gtgatcgggc ccaacggctc cggcaagacc acgctcttca
     5101 acctgatcac cgggctcatc gcgccggacg gagggagcat ccggctggag ggccgcgaga
     5161 tc
//
LOCUS       DATMCV010000410         3721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_685033, whole genome shotgun sequence.
ACCESSION   DATMCV010000410 DATMCV010000000
VERSION     DATMCV010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685033"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="VNU03_09515"
     CDS             <1..1575
                     /locus_tag="VNU03_09515"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HWP74743.1"
                     /translation="FISLMPESMHMIMWVMSDRAIPRSLRMMEGFGVHTFRLVDARGT
                     ARFVKFHWKPKLGVHSVLWDEALKISGKDPDFHRRDLWEAIAAGAYPEWELGLQVIDE
                     ADEHKFAFDLLDPTKVVPEEQVPVERVGRLVLDRNPDNFFAETEQVAFHPGHLVPGIE
                     FTDDPLLQGRLFSYTDTQLTRLGGPNFHEIPINRPVAGMHNNQRDGHMRQNINVGRVA
                     YHPNTLGGGCPMQAGRAAGGFATHPEAMDGRKVRERGEKFFDHFSQATLFWNSQSDPE
                     KQHIVRALRFELGKLEAAAVRERMVGMLAQVDRTLAGQVAEGLGLAVPKLAGPLNLSV
                     PADADPKKFQPIALKQAAVARSEALSMAQTVMDSAATRKVAILAAHGVDQDAVGAMQR
                     ALLAAGAVPKIVAPHGGSLKTGDGGALAVDFTLLTVGSVLFDAVFIPGGAPSTSALAG
                     DAAAVLFVREAYKHCKAIAASGTGLAVLEAAGVDVGEVLTRREGANGQLTAAFIKAIA
                     QHRDWSREAKAQTVPA"
     gene            1587..2210
                     /locus_tag="VNU03_09520"
     CDS             1587..2210
                     /locus_tag="VNU03_09520"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HWP74744.1"
                     /translation="MGRWPDVVGATDESRLPPSGLPPSGLPPNIQRVAKLEEAQLRAR
                     TISERIAAAVTRAAGTATFAIAHLVWFTVWIAVNGKWAHRLEPFEPFDPFPFNLLTML
                     VSLEAIFLSIWILISQNQMTRQAERRAHLDLQINLLAEQESTATLRIVHDIAEHLGIG
                     DRASRRDAALVEETDIEHLVSRMDEALPDTPGTSPSEPPRPGGMASP"
     gene            2256..2498
                     /locus_tag="VNU03_09525"
     CDS             2256..2498
                     /locus_tag="VNU03_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HWP74745.1"
                     /translation="MDIDEQVADSSVQAGLGRAGAVVKDVADKAREKFASYREGGLDQ
                     ISGDIEDYARRQPATALLIAAGVGLLVGMLLRPGRE"
     gene            2513..3544
                     /locus_tag="VNU03_09530"
     CDS             2513..3544
                     /locus_tag="VNU03_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWP74746.1"
                     /translation="MRSFGATLLAVSLLGVVGLGVAGSAEAQGVAELFGKVASTVVVV
                     RAKDGGADAQGRVSDIGSGVVVSGDGKIMTAAHLVHGAEGISVQFLGGRTVGARVVAS
                     ELAADLSLIQLAQVPAGLQPATLANSDAVRVGEQVIVVGAPYGLGHALSVGWISARWA
                     PSTVYKAIPLAEFFQTDASINVGNSGGPMFNMAGEVIGIVSHVISKSGGSEGLGFVVT
                     MNTAKQLLLERRSIWSGLEGQLLSDDLADLFNLPPKTSGFLVKTVAAGSPGEAMGLKG
                     GRKTATIDGQSLVVGGDIILTVEGIAVTTAADLAKIRERTSRLASAAPVAVTVLRAGR
                     QLKLTGKMP"
BASE COUNT          593 a         1245 c         1349 g          534 t
ORIGIN      
        1 ttcatctcgc tgatgcccga gtccatgcac atgatcatgt gggtcatgtc ggaccgggcc
       61 attccccgga gcctccggat gatggagggg ttcggcgtcc acaccttccg gctcgtcgac
      121 gcccggggca ccgcgcgctt cgtgaagttc cactggaagc cgaagctcgg ggtgcactcg
      181 gtgctgtggg acgaggcgct caagatctcg ggcaaggacc cggacttcca ccgccgcgac
      241 ctgtgggaag ccatcgccgc gggggcgtat ccggagtggg agctcggcct gcaggtcatc
      301 gatgaggccg acgagcacaa gttcgccttc gacctcctgg acccgaccaa ggtcgtgccc
      361 gaggagcagg tcccggtcga gcgggtgggc cgcctcgtgc tcgatcggaa cccggacaac
      421 ttcttcgccg agaccgagca ggtggcattc catcccggcc acctggtgcc cggcatcgag
      481 ttcaccgacg acccgctcct gcagggtcgg ctcttctcct acaccgacac ccagctcacg
      541 cgcctgggcg ggcccaactt ccacgagatc ccgatcaacc ggccggtggc ggggatgcac
      601 aacaatcagc gcgacgggca catgcggcag aacatcaacg tcggacgcgt cgcctaccat
      661 cccaacaccc tcggcggcgg ctgcccgatg caggccggcc gggccgccgg cgggttcgcc
      721 acccatccgg aggcgatgga cggccggaag gtgcgggagc gtggcgagaa gttcttcgac
      781 cacttcagcc aggcgacgct gttctggaac agccagtccg atccggagaa gcagcacatc
      841 gtccgggccc tccggttcga actcggcaag ctggaggcgg cggcggttcg cgagcggatg
      901 gtgggcatgc tggcccaggt ggaccgcacg ctggccggcc aggtggccga ggggctggga
      961 ctcgcggtgc cgaagctggc cggcccgttg aacctgagcg tgccggccga cgccgatcca
     1021 aagaagttcc agccgatcgc tctgaagcag gcggcggtcg ctcggtcgga agccctgagc
     1081 atggcgcaga ccgtcatgga ctccgcggcg acccggaagg tggccatcct ggccgcccat
     1141 ggcgtcgacc aggacgcggt cggggccatg cagcgggccc tgctggccgc aggggcggtg
     1201 ccgaagatcg tggcccccca cggcggctct ctcaagaccg gggacggggg cgccctggcg
     1261 gtcgacttca cgcttctcac cgtcggctcg gttctcttcg atgccgtatt catccccggg
     1321 ggcgctccgt cgacgagcgc gctcgccggc gacgcggccg ccgtgctctt cgtgcgcgag
     1381 gcctacaagc actgcaaggc catcgcggcg agcggaaccg ggctcgcggt cctggaagcc
     1441 gccggggtgg acgtgggaga ggtgctgacc cggcgggagg gggccaacgg ccagctgacc
     1501 gccgccttca tcaaggccat cgcccagcat cgggactgga gtcgggaagc caaggcccaa
     1561 accgtgccgg catgagggct gcccacatgg ggcgctggcc ggacgtggta ggagcgaccg
     1621 atgagtcgag gctcccgcct tcggggctcc caccctccgg cctcccaccc aatatccagc
     1681 gggtcgccaa gctcgaggag gcgcagctcc gggcccggac gatcagcgag cgcatcgcgg
     1741 ccgcggtcac ccgggcggcc gggaccgcga ccttcgccat cgctcacctc gtctggttca
     1801 cggtgtggat cgccgtcaac ggcaagtggg cgcaccgcct cgagccgttc gagcccttcg
     1861 atccctttcc gttcaacctc ctgaccatgc tggtgtcgct ggaggcgatc ttcctgtcca
     1921 tctggatcct gatcagtcag aaccagatga cgcggcaggc ggagcggcgc gcgcacctcg
     1981 acctgcagat caatctgctc gccgagcagg agtcgaccgc cactctccgc atcgtgcacg
     2041 acatcgcgga gcatctcggc atcggggacc gcgcgtcgcg tcgggacgcg gcgctcgtcg
     2101 aggagacgga catcgagcat ctggtgtcga ggatggacga agcgctaccg gataccccag
     2161 ggacgtcccc gtcggagccc ccacggcccg gcggcatggc ctccccctag cgggggaggg
     2221 gcgagcagct caccacaagc cagagggagc gaaccatgga catcgacgaa caggtggcgg
     2281 actcgagcgt gcaggcgggt ctcggacggg cgggagcggt cgtgaaggac gtggccgaca
     2341 aggcccgcga gaaattcgcc agctaccggg agggcgggct cgatcagatc tcgggggaca
     2401 tcgaggacta cgcgcgccgc cagcccgcga ccgccctcct cattgccgcc ggagtcgggc
     2461 tgctggtcgg gatgctgctg cgtccgggtc gagagtaggc ggccggccgg gaatgcgctc
     2521 cttcggggcc accctgctgg ccgtatccct cctcggagtc gtaggcctcg gagtcgcagg
     2581 gtcggcggag gcgcaggggg tcgcggagct gttcggcaag gtcgcctcca ccgtggtggt
     2641 ggtccgggcc aaggacggtg gggcggacgc gcaagggcgg gtcagcgaca tcggctccgg
     2701 cgtcgtggtt tcgggcgacg ggaagatcat gaccgcggcg cacctggtgc acggggcgga
     2761 gggcatcagc gtccagttcc tgggaggccg caccgtgggc gcccgggtcg tggcctcgga
     2821 gctggccgcc gacctgtccc tcatccagct ggctcaggtg ccggccggcc tgcagcccgc
     2881 gacgctcgcc aactcggacg cggtgcgggt gggcgagcag gtgatcgtgg tgggcgcccc
     2941 ctacgggctg gggcacgccc tcagcgtcgg ctggatcagc gcccggtggg ccccgagcac
     3001 ggtgtacaag gcgattccgc tcgccgagtt cttccagacc gacgccagca tcaacgtcgg
     3061 caactccggc gggccgatgt tcaacatggc gggagaggtc atcggcatcg tgagccacgt
     3121 catctcgaag agcggcggca gcgagggcct cggcttcgtg gtgacgatga acactgcgaa
     3181 gcagctactc ctggagcggc gctccatctg gtccggcctc gagggtcagc tcctgtccga
     3241 cgacctggcc gacctgttca acctgccccc gaagacgagc gggttcctcg tgaagaccgt
     3301 cgcggcggga tcacccggcg aggcgatggg gctcaagggc ggacggaaga ccgcgaccat
     3361 cgacggccag tcgctggtgg tcggggggga catcattctc accgtcgagg gcatcgcggt
     3421 cacgaccgcg gccgatctgg ccaagattcg cgagcgcacg agccggctgg ccagcgcggc
     3481 tccggtggcg gtgacggtgc tgcgggcggg tcggcagctg aagctcaccg ggaagatgcc
     3541 gtagcggccg ctcgctcgag cggcgaggag atatcgatgc cggcaccgaa gacggtcaag
     3601 cggcggacgc agaaggacgc ggtggccatt ctcaaggagg atcatgtgag ggtccgtaag
     3661 ctgctcggcg agctcgtgaa gaccgggcgc agctgctcgc caccatcgag cacgagctca
     3721 a
//
LOCUS       DATMCV010000411         2443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_221900, whole genome shotgun sequence.
ACCESSION   DATMCV010000411 DATMCV010000000
VERSION     DATMCV010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221900"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VNU03_09535"
     CDS             complement(<1..703)
                     /locus_tag="VNU03_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP74747.1"
                     /translation="MTDGLVKTDRHTTAFLEAGAPDAPLIVFVHGWPELSLSWRHQLP
                     CFADLGFRAVAPDMRGYGRSSVYRRHEDYALEHAVADMIELLDGLGREKAVWVGHDWG
                     SPVVWSLASHHPERCWGVANLCVPYVAAGFSPRNIIPLVDRTIYPEAQYPAGQWDYML
                     FYEESFEKACAAFDADPRNTVKALFRKGSPSGKGKPSRTAQTRKDGGWFGGAGRAPDV
                     PMDADVVTAEELETYA"
     gene            complement(729..1922)
                     /locus_tag="VNU03_09540"
     CDS             complement(729..1922)
                     /locus_tag="VNU03_09540"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP74748.1"
                     /translation="MSKPFAGVRILDFTRYLAGPYGTYQLALLGADVVKIESHEGDET
                     RISPADPKWAERKMAPSFLSMNGNKRSITLDLRRKEAIEIVKRLVVGADVVWENFRGG
                     VMDRLGLGYEALSAINPRLIYCGVSGFGRTGPEKATAAFDGKLQAMSGIMSITGEPAS
                     GPTRAGFALCDTIGGITAALAVASALYQRTHTGRGQLVDVAMLDAALAFIAGPVSEYT
                     VAGVEQKQIGNGSVSRKPTASRFRCGGGYIVLAVLTEKQFTGLLKTLGRADALADPRF
                     KDWAARTANEPALRQIIEAAMTEGDPRGWETRLTAADVPCGSIWKIDEIVHHPQLEHR
                     DVLQTIDSRYGPMRLVGAGFRLEHDGPGIDREPPTLGEHTDEILAEAGYTAEEIAQLR
                     RDGVA"
     gene            complement(1919..>2443)
                     /locus_tag="VNU03_09545"
     CDS             complement(1919..>2443)
                     /locus_tag="VNU03_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2306..2308),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HWP74749.1"
                     /translation="IGGLGPRFHGAPTNRSHRVTMESDAPELLRRCREDGADVAVLVP
                     SUPVCHQTVGLVARHLEANGLSTVIMGCAKDIVEHCGVPRFVWSDFPLGNSAGRPFDV
                     ESQARTLELALRLLESAAAPRTTVASPLRWTADAAWKHRYLDVGKLSPEDIAERRKEF
                     EVVKAVAKAKREGT"
BASE COUNT          328 a          877 c          871 g          367 t
ORIGIN      
        1 ccgcgtaggt ctcgagctcc tccgcggtca ccacgtcggc atccatcggc acgtcgggcg
       61 cccggccggc gccgccgaac cagccgccgt ccttgcgggt ctgggcggtg cgcgacggct
      121 tgcccttgcc gctcgggctc cccttccgga acagggcctt gaccgtgttg cggggatcgg
      181 cgtcgaaggc ggcgcaggcc ttctcgaagc tctcctcgta gaagagcatg tagtcccact
      241 gccccgccgg gtactgcgcc tccggataga tcgtgcggtc caccagcggg atgatgttcc
      301 tcggtgagaa ccccgccgcg acatacggca cgcagaggtt cgccacgccc cagcagcgct
      361 cgggatggtg gctggccagg ctccacacca ccgggctgcc ccagtcgtgg ccgacccaca
      421 ccgccttctc ccggccgagc ccgtcgagca gctcgatcat gtcggccacc gcgtgctcga
      481 gcgcgtagtc ctcgtggcgg cggtacacgc tcgagcggcc gtagccgcgc atgtcggggg
      541 cgaccgcgcg gaagccgagg tcggcgaagc acgggagctg gtggcgccag gagagcgaca
      601 gctccggcca gccgtggacg aacacgatca ggggagcgtc gggagcgccc gcctcgagaa
      661 aggcggtggt gtgccggtcg gtcttcacga ggccgtcggt caccgcggat ctcatcgggc
      721 gcccgcggtc aggccacgcc gtcgcgccgg agctgcgcga tctcctcggc ggtgtacccg
      781 gcttccgcca ggatctcgtc ggtgtgctcg ccgagggtcg gcggctcccg atcgatcccg
      841 ggcccgtcgt gctcgaggcg gaagcccgcc cccaccagcc gcatcggccc gtagcgggag
      901 tcgatggtct ggagcacgtc gcggtgctcg agctgcgggt ggtggacgat ctcgtcgatc
      961 ttccagatgg agccgcacgg cacgtcggcc gcggtgaggc gcgtctccca gccgcgggga
     1021 tcgccctcgg tcatggcggc ctcgatgatc tggcgcaggg ccggctcgtt ggcggtgcgg
     1081 gccgcccagt ccttgaagcg tgggtcggcg agcgcgtcgg cgcgaccgag cgtcttcagc
     1141 aggccggtga actgcttctc ggtgaggacg gccaggacga tgtatccgcc gccgcaccgg
     1201 aaccggctcg cggtcggttt gcggctcacc gagccgttgc cgatctgctt ctgctcgacg
     1261 ccggccaccg tgtactcgga caccggcccc gcgatgaagg ccagcgccgc gtccagcatc
     1321 gccacgtcaa cgagctggcc gcgcccggtg tgggtccgct ggtagagcgc gctcgccacc
     1381 gccagggccg cggtgatccc gccgatggtg tcgcagagcg cgaagcccgc gcgggtgggg
     1441 ccggaggccg gctcgccggt gatcgacatg atgccagaca tcgcctggag cttgccgtcg
     1501 aaggccgcgg tggccttctc cgggccggtg cggccgaagc cggagacgcc gcagtagatg
     1561 agcctggggt tgatggcgga cagcgcctcg tacccgagcc ccagccggtc catgaccccg
     1621 ccccggaagt tctcccagac cacgtcggcg ccgaccacca ggcgcttgac gatctcgatc
     1681 gcctctttcc gccgcaggtc cagcgtgatc gatcgcttgt tgccgttcat ggagaggaac
     1741 gacggggcca tcttccgctc cgcccacttg ggatcagccg gcgagatgcg ggtctcgtcc
     1801 ccctcgtgcg actcgatctt gacaacgtcg gcgcccagga gggcgagctg gtaggtcccg
     1861 taggggccgg cgagatagcg ggtgaagtcc aggatgcgga cccccgcgaa gggcttgctc
     1921 acgtgccctc gcgcttggcc ttcgccaccg ccttcaccac ctcgaactcc ttccgccgct
     1981 ccgcgatgtc ctccggcgag agcttcccca cgtcgaggta cctgtgcttc caggccgcgt
     2041 ccgcggtcca gcgcagcggc gacgccaccg tggtccgggg ggcggccgcg ctctcgagca
     2101 gccgcagggc cagctcgagg gtgcgcgcct gggactccac gtcgaacggc cgccccgcgg
     2161 agttgccgag cgggaagtcg ctccatacga agcgcgggac gccgcagtgc tcgacgatgt
     2221 ccttggcgca gcccatgatc acggtcgaca ggccgttcgc ctcgagatgc cgggcgacca
     2281 gacccacggt ctggtgacag accggtcagc tggggacgag gaccgccacg tcggcgccgt
     2341 cctcgcggca gcgccgcagc agctcgggcg cgtcgctctc catggtcacg cgatggctcc
     2401 ggttggtcgg cgccccgtga aagcgcgggc ccaggccgcc gat
//
LOCUS       DATMCV010000412         9217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_20577, whole genome shotgun sequence.
ACCESSION   DATMCV010000412 DATMCV010000000
VERSION     DATMCV010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20577"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..372)
                     /locus_tag="VNU03_09550"
     CDS             complement(166..372)
                     /locus_tag="VNU03_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74750.1"
                     /translation="MKWPSSRSVALVWLLAALSLIGWLIVNLDTAGWIILTLTVGLMA
                     FELTRRRDGSGGQPKRRIPDEPTG"
     gene            complement(430..1269)
                     /locus_tag="VNU03_09555"
     CDS             complement(430..1269)
                     /locus_tag="VNU03_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP74751.1"
                     /translation="MKRAGSRKTAPASGIFLTGIRRAGVLREPDRFPFTIPLLRGLDR
                     LDLRARVTFLVGENGCGKSTLLEGIAAGVGAVAAGGRDLERDPTLAGARAFARAFRCR
                     RARHPRTTLFLRAEDVFGFTLRVRRDIEGLGEVEDELRGALREGSWGQRLATGAAAGQ
                     RAALIRRYGADPDARSHGETFLHLLQQRLQPRGLYFLDEPETPLSPTRVLALLAILRD
                     RAAQDCQFVIATHSPILMALPGAEILLLEGETIAPIDYREVEHVRITRAFLDDPESFL
                     RRL"
     gene            complement(1283..2254)
                     /locus_tag="VNU03_09560"
     CDS             complement(1283..2254)
                     /locus_tag="VNU03_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HWP74752.1"
                     /translation="MATKILVSPKFPAAMLEIARSFVPAGFEMVVVDAGTPEFTAALK
                     DAEYYVGFPRPELNSEFFKTASGLKLVQLISAGYDRLDVAAAKRAGVPVANNGGSNSV
                     AVAEHTLMLMLAVYRKLSWHHGNVITGKWRVGDFASNRTYELEGKTLGIIGLGTIGKK
                     VARRAAAFDMKIVYYDIARLAEDAEDALGVRFVLLPELLRTADIVSLHVPLNTVTRNM
                     FGARELGMMRPTAVLINTCRGPVVDEKALHTALTTKVIAAAGLDVMVEEPPIANHPLF
                     ALENVTFTPHTAGPTWDNWFKAFRNAFDNVQRVARGDRPLWVIPELR"
     gene            2515..2757
                     /locus_tag="VNU03_09565"
     CDS             2515..2757
                     /locus_tag="VNU03_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74753.1"
                     /translation="MKRFVVTLAWVLWAHELAQVGDRLVDRGYTAVDSFETRQQCHAA
                     LADYSALKLVRQGKVKVEFTCLPEKTEPRPPRTAVG"
     gene            complement(2773..4005)
                     /locus_tag="VNU03_09570"
     CDS             complement(2773..4005)
                     /locus_tag="VNU03_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP74754.1"
                     /translation="MHLMYFTEQPMSAYPADEGRKRGATALMFSNRHFDPVAGSRLYN
                     EYLEHYRLAEEVGFDGIMLNEHHNAPFCMQAKCNVFASILAAMTKRVKIVLLGNPLPL
                     ADNPVRLAEELAMIDMVSRGRLVSGFVRGGGQEQLATGVNPAFNRERFEEAHELIVRA
                     WTQEGPFRFEGNHYQHRVVNPWAVPLQKPHPRIWIPGVLSKETIIWAARQRYPYIALS
                     TAIDATKKIWELYDSVAAEAGYTGGPENRGYLQRCHVAETEEKALANARQFMWMQGEF
                     TGLAHPVWSSPSGYFSPSYRKAFVEYAVGRGSNPRAASFEDQIRDQQIIAGTPKTVLP
                     KFRRLLEETRPSILGLWASDGFVSNQDATTCIRLLGQEVLPAVREMGKELGLWSPFEA
                     NAPVSITQPAPAVHRNGA"
     gene            complement(4019..4816)
                     /locus_tag="VNU03_09575"
     CDS             complement(4019..4816)
                     /locus_tag="VNU03_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP74755.1"
                     /translation="MTPTASALAWTTEEVRVAGSSVRLRTTGRGAPLLVLHHDIGTPE
                     QLPFYEALAQRFTVLLPSHPGYDGSERPAWMRSVRDVAVTYQALLAARGLADVSLVGL
                     GFGGWIAAEMATMSPRAFRRLVLVGAMGIKPAEGEILDQALVSYIDYARAGFADQAAF
                     DRLFGADPPTPQLEQWDLNREMTFRIAWKPYMYSPTLPPLLEGVQTETLVVWGSDDRV
                     VPAASGEQYARALPRSRLVVVEGAGHLVDMEQPEALAKLIVQFVGQS"
     gene            complement(4864..5718)
                     /locus_tag="VNU03_09580"
     CDS             complement(4864..5718)
                     /locus_tag="VNU03_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HWP74756.1"
                     /translation="MLIAQITDTHISTPGSVNDRHFRTPEHLERAVAHLNRLTPRPDV
                     VLATGDLVERGEPEEYARLRAILDRLAMPLYVIPGNHDSREGLARAFADRGYLPTDGG
                     FLHYTVEEWPVRLIGLDTQVPGQPGGRLCAERLAWLDARLGEAAARPTVVFMHHPPFV
                     TGLQAMDAMGLEGKEELATVIRRHPQVERLVCGHVHRPMTRRFAGTVATTCPATAHQI
                     ALELPPTPRLSVVMEPPACMLHLWLGADAGLVSHVSVIGDERPPFTLFDGQRWLREAV
                     PPPAFHPG"
     gene            5983..7062
                     /gene="asd"
                     /locus_tag="VNU03_09585"
     CDS             5983..7062
                     /gene="asd"
                     /locus_tag="VNU03_09585"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWP74757.1"
                     /translation="MKKRVAVVGATGIAGQQFLVALAGHPWFEVALLAASERSAGKTY
                     AEALRDPRSGARRWWCLEEPPAELLDRPVERADGLNLAGIDIVFAAVESDAARVLEPQ
                     YARTTPVLSTASAFRYEADVPILVPGVNPAHAGLIEHQRRARGWRGFIVPLPNCTTMG
                     LVVTLRPLLDRFGIERVLMTSMQGLSGAGRSPGVIALDVLDNIIPYIPGEEEKVARET
                     GKILGRMAASGIVPTAFPVSATCTRAAVSEGHTEAVSVSLGAPAEPADVAAALSEFDG
                     GLGGLDLPSAPRRLIRVHDDPFRPQPRLDRDVDGGMATSVGRIREDPALERGVKYVLV
                     SHNTKMGAAKGAVLTAEYLATSGHL"
     gene            7157..8143
                     /locus_tag="VNU03_09590"
     CDS             7157..8143
                     /locus_tag="VNU03_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HWP74758.1"
                     /translation="MAGTGTLKYGVVEGWEQLPKGYVHRDVAGVAVDSEDRVYLICRG
                     DHPIIAYDRDGKFLGSWGEGQFTYRTHGITVGPDQTLYCTDDGNHTIRKFTPKGKLLM
                     TLGTMDKPADTGYDGKDYMTITKPADGFNRPTNIAVGPKGDLYVSDGYGNCRVHQFGP
                     NGERKRSWGVPGTGPGQFYLPHGIAAAADGRIFVCDRENDRIQIFSPDGEYLSEWTDT
                     QRPTHLVFDGQGRAYVSELWWHKGDPAPRQGRTITEPLYGRVSVFDRDGRLLTRWGTP
                     EATAPGSFAAPHGIAVDSTGAIYVAEVTWTFAESRGRVPAGCHTFQKFTLKG"
     gene            8177..8611
                     /locus_tag="VNU03_09595"
     CDS             8177..8611
                     /locus_tag="VNU03_09595"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HWP74759.1"
                     /translation="MGAFKLAKGLALVTAGVTALYLAGHDSAQTLTRWVARLHLDPGA
                     HWIDRPLAAALALDPRSLRAIGLGTFVYAAVFLTEGVGLLLRRHWAEYLAVVATASFL
                     PLEVYELIQHVRPTQLLIVAANLAIVLYLIWRLRTEHGRHRE"
     gene            8614..9099
                     /locus_tag="VNU03_09600"
     CDS             8614..9099
                     /locus_tag="VNU03_09600"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWP74760.1"
                     /translation="MSYTPSEKIGTLTDDEAVAFLEEPWTGRIATVDGEGWPYVAPVW
                     YEFERERRSFLVVGREASRWIAHIRVNPRVAFHVADDLHAHHTRVQVQATARILEGPV
                     APTASPALLELTHRLSRRYLGPDGPRYAERTLARPRVLVRLEPVRWTTWTGQEWHPRY
                     R"
BASE COUNT         1301 a         3249 c         3337 g         1330 t
ORIGIN      
        1 ggctgcagcc gcccatgacg gagaacgagc cccgctcgcg gctgctgcgc tgggtcatct
       61 ggctcgcgac gctgttcttc gtcgcgatgc tcttcgttcc gctcgtccgc gaggcgctgc
      121 gccgcgccgc gctggtcccg cccggccact tctagcgcgg gtgcgctagc cggtcggctc
      181 gtcggggatc cggcgcttgg gctggccccc gctcccgtcc cgtcggcgcg tcagctcgaa
      241 ggccatcagc ccgaccgtga gcgtgaggat gatccagccc gcggtgtcga ggttgacgat
      301 cagccagccg atgaggctga gggcggcgag gagccacacc agggccacgg agcgggaact
      361 gggccacttc atcggactct attgtcgcgg cggtcggcgc cgaagtcaaa ccgacgcgcg
      421 ctcacgcgct cagaggcggc ggaggaagct ctcgggatcg tcgaggaagg cgcgcgtgat
      481 gcggacgtgc tcgacctcgc ggtagtcgat cggcgcgatc gtctcgccct cgagcagcag
      541 gatctcggcc cccggcagcg ccatcaggat cggcgagtgg gtggcgatga cgaactggca
      601 atcctgggcc gcgcggtcgc gcaggatcgc gagcagcgcc agcacgcggg tcggcgagag
      661 cggggtctcc ggctcgtcga ggaagtagag gccgcgcggc tgcagccgct gctggagcag
      721 gtgcaggaac gtctccccgt gcgagcgcgc gtcgggatcc gccccgtagc gccggatcag
      781 ggccgcgcgc tgccccgccg cggcgccggt ggcgaggcgc tggccccacg agccctcgcg
      841 cagggcgccc cgcagctcgt cctccacctc gccgagcccc tcgatgtcgc gccgcacgcg
      901 cagggtgaag ccgaagacgt cctccgcccg caggaagagc gtggtgcgcg ggtgccgggc
      961 ccgccggcac cggaaggcgc gggcgaaggc gcgcgccccg gccagggtgg gatcgcgctc
     1021 gaggtcgcgg ccgcccgcgg cgaccgcccc caccccggcg gcgatcccct cgagcagggt
     1081 cgacttgccg cagccgttct cccccaccag gaaggtgacg cgcgcccgga ggtcgagccg
     1141 gtccaggccc cggaggagcg ggatcgtgaa cggaaagcga tcgggctccc gcagcacgcc
     1201 ggcgcggcgg atcccggtga ggaagatccc cgacgccggc gccgtcttgc gggagcccgc
     1261 gcgcttcact cggtcccggc ggctagcgaa gctccgggat cacccagagc ggccggtcgc
     1321 cgcgggccac tcgctgcacg ttgtcgaagg cgttccggaa tgccttgaac cagttgtccc
     1381 aggtggggcc cgcggtgtgc ggggtgaacg tgacgttctc gagcgcgaac agcgggtggt
     1441 tggcgatcgg cggctcctcc accatgacgt cgagcccggc cgccgcgatg accttggtgg
     1501 tgagcgcggt gtggagtgct ttctcgtcca ccaccgggcc ccggcaggtg ttgatgagca
     1561 ccgcggtggg ccgcatcatg ccgagctcgc gcgccccgaa catgttgcgg gtgaccgtgt
     1621 tgagcggcac gtggagcgag acgatgtccg cggtgcgcag cagctcgggc aggagcacga
     1681 agcggacgcc gagggcgtcc tcggcgtcct cggcgaggcg cgcgatgtcg taatagacga
     1741 tcttcatgtc gaaggcggcg gcccggcgcg cgaccttctt gccgatggtg ccgagcccga
     1801 tgatgccgag ggtcttgccc tcgagctcgt aggtccggtt cgaggcgaag tcccccaccc
     1861 gccacttgcc ggtgatcacg ttgccgtggt gccaggagag cttgcggtag accgcgagca
     1921 tcagcatgag ggtgtgctcg gccaccgcga ccgagttgga gccgccgttg ttggccaccg
     1981 gcacccccgc ccgcttggcc gccgccacgt cgagacggtc gtagccggcg ctgatgagct
     2041 gcaccagctt caggcccgag gccgtcttga agaactcgct gttcagctcg gggcgcggga
     2101 agcccacgta gtactcggcg tccttgaggg ccgcggtgaa ctcgggcgtg cccgcgtcca
     2161 ccacgaccat ctcgaagccg gcgggcacga agcttcgcgc gatctcgagc atcgcggcgg
     2221 ggaacttggg agagaccagg atcttggtgg ccacggcagt ctcctttacg tgacaggggt
     2281 gattggcggc gtcacgcgcg ccagtatacg gcttcgcccg gcgccgtgtc cgccgcgtgt
     2341 gcggtcggca tctcttgcgc gggggcggtc gcctcaggct gggtactggc ctgcccagcc
     2401 tcataccccc gcttctctgc aactctgagg cgatcggcac caagtgtccg aaagactgtc
     2461 tgttttcaga tggtcccaag cttgctctat ccggggcgta caaaggggga ttccatgaag
     2521 cgattcgtcg tcacgctggc ctgggtgctg tgggcgcacg agctggcgca ggtgggcgac
     2581 cggctcgtcg accggggcta caccgcggtc gactcgttcg agacccggca gcagtgccac
     2641 gccgccctgg ccgactactc ggccctcaag ctcgttcgtc agggcaaggt gaaggtcgag
     2701 ttcacctgcc tgcccgagaa gaccgagccc cggcctcccc gcaccgcggt cggctgagcg
     2761 ccggctcccg gctcacgccc cgttgcggtg caccgccggg gcgggctggg tgatgctcac
     2821 cggagcgttg gcctcgaagg ggctccagag cccgagctcc ttgcccatct cgcgcaccgc
     2881 cgggagcacc tcctgcccga gcaggcggat gcaggtggtc gcgtcctggt tggacacgaa
     2941 cccgtcgctg gcccagagcc ccaggatgga cggccgcgtc tcctcgagca ggcgccggaa
     3001 cttcgggagc accgtcttcg gggtgcccgc gatgatctgc tggtcgcgga tctgatcctc
     3061 gaaggaggcc gcgcggggat tcgagccgcg tcccaccgcg tactcgacga aggccttccg
     3121 gtaggacggt gagaagtagc cggacgggct cgaccacacc gggtgcgcga gcccggtgaa
     3181 ctcgccctgc atccacatga actggcgcgc gttggcgagc gccttctcct cggtctcggc
     3241 cacgtggcag cgctgcaggt atccgcggtt ctcggggccg ccggtgtagc cggcctcggc
     3301 cgccaccgag tcgtagagct cccagatctt cttggtcgcg tcgatcgcgg tgctgagcgc
     3361 gatgtagggg tagcgctggc gcgcggccca gatgatcgtc tccttgctga gcacgcccgg
     3421 gatccagatg cgggggtgcg gcttctgcag cggcaccgcc cacgggttca ccacccggtg
     3481 ctggtagtgg ttgccctcga agcggaacgg gccctcctgg gtccacgcgc gcacgatcag
     3541 ctcgtgggcc tcctcgaagc gctcgcggtt gaacgcgggg ttgaccccgg tggcgagctg
     3601 ctcctgcccg cccccgcgca cgaagccgga gacgaggcgg ccgcgcgaga ccatgtcgat
     3661 catggccagc tcctcggcca gccgcaccgg gttgtcggcg agcggcagcg ggttgccgag
     3721 cagcacgatc ttcacccgct tggtcatggc ggccaggatc gaggcgaaca cgttgcactt
     3781 ggcctgcatg cagaacggcg cgttgtgatg ctcgttcagc atgatgccgt cgaagccgac
     3841 ctcttccgcg aggcggtagt gctcgaggta ctcgttgtag agccggctgc ccgccaccgg
     3901 atcgaagtgg cggttggaga acatcagcgc ggtggcgccg cgcttgcggc cctcgtccgc
     3961 ggggtaggcc gacatcggct gctcggtgaa gtacatgagg tgcatgggga tcccccgctc
     4021 aggactggcc gacgaactgg acgatgagct tcgcgagcgc ctcgggctgc tccatgtcga
     4081 ccaggtggcc ggcgccctcc accaccacga gccggctccg gggcagcgcg cgcgcgtact
     4141 gctcgccgct cgcggccggc acgacccggt cgtcgcttcc ccacaccacc agggtctcgg
     4201 tctgcacccc ttcgagcagc ggcggcagcg tcgggctgta catgtagggc ttccacgcga
     4261 tgcggaacgt catctcgcgg ttgaggtccc actgctcgag ctgcggggtg ggcggatccg
     4321 cgccgaagag ccggtcgaac gcggcctggt ccgcgaagcc cgcgcgggcg tagtcgatgt
     4381 agctgaccag cgcctggtcg aggatctccc cttccgcggg cttgatcccc atcgcgccca
     4441 ccagcaccag ccggcgaaac gcgcgcggcg acatggtggc catctcggcc gcgatccacc
     4501 ccccgaagcc gagccccacc agcgagacgt cggctaagcc gcgggcggcg aggagggcct
     4561 gataggtgac cgcgacgtcc cgcacgctcc gcatccacgc gggccgttcg gagccgtcgt
     4621 agcccgggtg cgagggcagg agcaccgtga agcgctgcgc gagcgcctcg tagaacggca
     4681 gttgctccgg cgtcccgatg tcgtggtgga gcacgagcag cggggcgccg cgcccggtag
     4741 tacgtagtcg tacggaggag cccgccacgc ggacctcctc ggtggtccac gcgagagcgg
     4801 aggcggtggg ggtcatgctc gcgacgctat caaatcgacc ggaggcccgc aagcacgacc
     4861 ggctcagccc ggatggaagg cgggcggcgg caccgcctcc cgcagccacc gctgcccgtc
     4921 gaagagcgtg aagggcgggc gctcgtcgcc gatcacgctg acgtggctca cgagccccgc
     4981 gtccgcgccg agccagaggt gcagcatgca cgcgggcggc tccatgacga cggacagccg
     5041 cggggtgggc gggagctcga gcgcgatctg gtgcgcggtg gccgggcacg tggtggccac
     5101 cgtgcccgcg aaccgtcgcg tcatcgggcg gtgcacgtgg ccgcacacga ggcgctccac
     5161 ctgggggtgg cggcggatca cggtggcgag ctcctccttg ccctcgaggc ccatcgcgtc
     5221 catcgcctgg agcccggtca cgaacggcgg gtggtgcatg aacaccacgg tgggccgcgc
     5281 cgcggcctcg ccgagccgcg cgtcgagcca ggcgagccgc tccgcgcaga ggcgcccgcc
     5341 gggctggccg ggcacctgcg tgtcgaggcc gatcagccgc accggccact cctccaccgt
     5401 gtagtgcagg aagcctccgt ccgtcggcag gtagccgcga tcggcgaacg cgcgcgcgag
     5461 gccctcccgc gagtcgtggt tgcccgggat cacgtagagc ggcatcgcga gccggtcgag
     5521 gatggcgcgc aggcgcgcgt actcctcggg ctcgccgcgc tcgaccaggt cgccggtggc
     5581 cagcaccacg tccgggcgcg gcgtgagccg gttcaggtgc gccaccgcgc gctcgaggtg
     5641 ctcgggggtg cggaagtggc ggtcgttcac gctccccggc gtgctgatgt gggtgtcggt
     5701 gatctgggcg atgagcatgg ctcgatcata cattgacagc ggaccgcccc tggattacaa
     5761 gagagcccat gccgtaccgg gccacccgca agaccgacaa gacccggctg gggtccccga
     5821 ccgtcgacgc gacgggggga cgcccagcgg gaccggtggc gcgcgcccga agaccgacct
     5881 gaccgactcg atcacgttct cggatcctcg aggcccgcga ccaccacccg gtgatcgcgg
     5941 gcctcttgcg ttcggggact cgagggcata ggaggccccg tcatgaaaaa gcgtgttgcg
     6001 gtggtgggag cgaccggcat tgccgggcag cagttcctcg tcgcgctggc cgggcatcca
     6061 tggttcgagg tcgcgctgct ggccgcctcc gagcgctcgg ccggcaagac gtacgcggag
     6121 gcgctgcgcg atccccggag cggggcgcgg cgctggtggt gcctcgagga gccgcccgcc
     6181 gagctgctcg accggccggt cgagcgcgcg gacggcctca acctcgccgg catcgacatc
     6241 gtgttcgccg cggtcgaatc ggacgccgcg cgcgtcctcg agccgcagta cgcgaggacg
     6301 acgcccgtcc tcagcaccgc ctccgccttc cgctacgagg cggacgtgcc gatcctggtg
     6361 ccgggggtga atccggcgca cgccgggctg atcgagcacc agcgccgcgc ccgcggctgg
     6421 aggggcttca tcgttcccct gcccaactgc acgaccatgg ggctggtggt gacactcagg
     6481 ccactcctcg accgcttcgg catcgagcgg gtgctgatga cctccatgca gggactctcc
     6541 ggggccggcc gctcccctgg ggtgatcgcg ctcgacgtgc tcgacaacat catcccctac
     6601 atcccgggcg aggaggagaa ggtggcgcgc gagaccggca agattctcgg gcggatggcg
     6661 gcgagcggga tcgtgcccac cgcgttcccg gtcagcgcga cctgcacgcg ggccgcggtc
     6721 agcgaggggc acaccgaggc ggtctcggtc tcgctcggcg cgccggcgga gccggccgac
     6781 gtggccgccg cgctgtccga gttcgacgga ggcctcggcg gcctcgatct gccgtcggcg
     6841 ccgcggcggc tgatccgcgt gcacgacgat ccgttccggc cccagccgcg gctcgaccgc
     6901 gacgtggacg gcggtatggc cacgagtgtc ggccgcatcc gggaggatcc ggcgctcgag
     6961 cgcggcgtga agtacgtgct ggtgtcgcac aacacgaaga tgggcgccgc gaagggagcg
     7021 gtgctgaccg cggaatacct cgcgacgagc gggcatctct aggcggtgcc cggcttgact
     7081 cggctgggcc tttctcccat agtcggacgc ggcgggcgca gcgcattcga tcggacggaa
     7141 ccgggaggag aacccaatgg cggggacggg cacgctgaag tacggggtgg tcgagggctg
     7201 ggagcagctg ccgaagggct acgtgcatcg cgacgtggcg ggggtggcgg tggactcgga
     7261 ggaccgcgtc tatctgatct gccgcggcga ccatccgatc atcgcgtacg accgcgacgg
     7321 caagttcctc ggctcgtggg gcgagggcca gttcacctac cgcacccacg gcatcacggt
     7381 cggcccggac cagacgctct actgcaccga cgacggcaat cacacgatcc gcaagttcac
     7441 cccgaagggc aagctgctga tgaccctcgg cacgatggac aagcccgccg acaccggcta
     7501 cgacggcaag gactacatga cgatcacgaa gccggccgac ggcttcaacc gccccacgaa
     7561 tatcgcggtg ggcccgaagg gcgacctcta cgtgtccgac gggtacggca actgccgcgt
     7621 gcaccagttc ggccccaacg gagagcggaa gcgctcgtgg ggcgtgcccg gcaccgggcc
     7681 cggccagttc tacctgcccc acggcatcgc ggccgccgcg gacgggcgca tcttcgtctg
     7741 cgaccgggag aacgaccgca tccagatctt cagccccgac ggcgagtacc tctcggagtg
     7801 gaccgacacc cagcggccca cccacctggt gttcgacggt caggggcgcg cctacgtctc
     7861 cgagctctgg tggcacaagg gcgaccccgc gccgcgccag ggccgcacca tcaccgagcc
     7921 gctctacggg cgcgtcagcg tgttcgaccg cgacggccgg ctcctcaccc ggtggggcac
     7981 gcccgaggcg accgccccgg gtagcttcgc ggccccgcac ggcatcgcgg tcgactccac
     8041 cggcgcgatc tacgtggccg aggtgacctg gaccttcgcg gagagccggg gccgcgtgcc
     8101 ggcgggctgc cacacgttcc agaagttcac gctcaagggc tgaggcctga cgcgcggcga
     8161 tcggctgctc cggctcatcg gcgcgttcaa gctcgccaag gggctggcgc tggtgaccgc
     8221 gggggtgacc gcgctgtacc tcgccgggca cgactcggcc cagacgctca cccgctgggt
     8281 cgcgcgcctg cacctggatc cgggggccca ctggatcgac cggcccctcg cggccgcgct
     8341 cgcgctcgat ccccggtcgc tgcgcgcgat cgggctcggc acgttcgtct acgcggcggt
     8401 cttcctcacc gagggggtgg gcctcctgct ccggcgccac tgggccgagt acctcgcggt
     8461 ggtcgccacc gcgtccttcc tgccgctcga ggtgtacgag ctgatccagc acgtgcggcc
     8521 cacccagctg ctcatcgtcg ccgcgaacct cgccatcgtg ctctacctga tatggcgcct
     8581 gcgcaccgag cacggccggc accgggagtg accatgtcct acacgccgag cgagaagatc
     8641 gggaccctga ccgacgacga ggcggtcgcc ttcctcgagg agccgtggac cggccgcatc
     8701 gccaccgtcg atggggaggg ctggccgtac gtcgcgccgg tctggtacga gttcgagcgc
     8761 gagcggcgga gcttcctcgt ggtgggacgc gaggcctccc gctggatcgc ccacatccgc
     8821 gtcaacccgc gcgtggcctt ccacgtggcg gacgatctcc acgcccacca cacccgggtg
     8881 caggtgcagg ccaccgcgcg aatcctggag ggtccggtcg cgcccaccgc cagcccggcc
     8941 ctgctcgagc tgacccatcg gctctcgcgg cgctacctgg gccccgacgg tcctcgctac
     9001 gcggagcgca cgctcgcgcg gccccgggtg ctggttcgcc tcgagccggt caggtggacg
     9061 acctggaccg gccaggagtg gcacccgcgc tatcggtgag tcggctcagg agtcggctcg
     9121 tctctcccgc gggagtccgg tatcatcctt ggcatgtcgc tctcctccgg tgcgccggtc
     9181 accgccggcg acctgctcac gctggaggag ctgggcc
//
LOCUS       DATMCV010000413         6197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_554823, whole genome shotgun sequence.
ACCESSION   DATMCV010000413 DATMCV010000000
VERSION     DATMCV010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554823"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VNU03_09605"
     CDS             complement(<1..720)
                     /locus_tag="VNU03_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74761.1"
                     /translation="MDRAALARLRGWLARADLAAIDPEGVERLRLALDEPDPPVTVGR
                     GGTLTAYAPGPGRRRLLQLDRRGHLVAAWRWHPDGRLEWAKCLLPDGRWLGIEPAART
                     HPAWGLSDRVWQLEPDAPWAPREALTCFQAVEYARPDFIPPLAEPRRLPAGGGTALLN
                     LIASVMKDHGVATVRYRGPYPTEQLFTALLECFRYDAAEALPLERFLADGALDWHPAP
                     FETHQVTPGVVVQLRHEVDKVM"
     gene            747..1304
                     /locus_tag="VNU03_09610"
     CDS             747..1304
                     /locus_tag="VNU03_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HWP74762.1"
                     /translation="MSVTTRLAGPDDAAAIARIYNQGIEDRVATFETEPRTPEDIRAQ
                     LASKGDRYPTVVVERDGVVVAWASAGAYRARPAYAGVAEHSVYVDRSARGHGAGRAAL
                     DALFRCYEARGFWKLVSRIFPENVASLALHERAGFRTVGVYARHGKLAGEWRDCVIVE
                     KLLGEAAAASGSTPSPPPRPPAAPR"
     gene            complement(1216..1911)
                     /locus_tag="VNU03_09615"
     CDS             complement(1216..1911)
                     /locus_tag="VNU03_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HWP74763.1"
                     /translation="MTSGSERPDLPRRVTAEAVGTAFLLAAVIGSGIMGERLAGGNVA
                     VALLANTLATGAALVALILTFGEISGAHFNPAVTLVEAAGGGLAWRDVPVYVLAQIAG
                     AFAGVAVAHLMFGEPVFFASRHVRAGPAQLLGELVATFGLLAVIQGCARHRAAAVPYA
                     VAAYITAAYWFTSSTSFANPAVTLARAASDTFAGIRPADAPGFIAAQLVGAAAATAYF
                     RWLLRPPRAASRR"
     gene            complement(1908..2357)
                     /locus_tag="VNU03_09620"
     CDS             complement(1908..2357)
                     /locus_tag="VNU03_09620"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWP74764.1"
                     /translation="MTDHSSGRDRALILCTHNSARSQMAERFLRELGGDRFEVASAGT
                     VATAVHPLAARVMEEIGLDLRGHSSKTLDRYLGERWDYVITVCDQAGERCPLFPGPAR
                     RIHWSFEDPSAATGSEADRLATFRRVRDAIRDRLRAWLAEIAPRPAA"
     gene            2652..2981
                     /locus_tag="VNU03_09625"
     CDS             2652..2981
                     /locus_tag="VNU03_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP74765.1"
                     /translation="MSHYHDSDDLKVLGEFKKLAPAEFKGFVELDSIVGRDDGKIPRK
                     YRELIALAVACTTQCPYCLDVHTRNAKKAGATREEVAESALLAAALRAGAAVTHGALA
                     VKLFDGA"
     gene            3168..3491
                     /locus_tag="VNU03_09630"
     CDS             3168..3491
                     /locus_tag="VNU03_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74766.1"
                     /translation="MKRITGVAAGVMATFLAVGPGFAADAMRDSTSIEKPGKLAMPHR
                     VTGSVVSVDESARTFTVQDSNGKDYVLTADPALGSQLDQVKAGGHVKVSYKKNSSGQM
                     IATNR"
     gene            3671..4075
                     /locus_tag="VNU03_09635"
     CDS             3671..4075
                     /locus_tag="VNU03_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74767.1"
                     /translation="MNPRVIAIGIALCLGACAPVAVPMPIQPFPDVPVPAEWTPYSDD
                     WVMIRSPKITAARLIYFTRESVDETLAHARRLLARAGWIETHSERFVNAEKFSGVWVE
                     FGKDADVCRLTVIEGTGATHVDYTLARLNPGS"
     gene            4075..5487
                     /locus_tag="VNU03_09640"
     CDS             4075..5487
                     /locus_tag="VNU03_09640"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP74768.1"
                     /translation="MATTDLVWRSALELAALIRAKQVSPVEVVDAVLARIERLNPALN
                     CYATVTAEEARDAAVAAEVAVETGEELGPLHGVPVSIKDLVFTRRVLTTGGSRLFTDH
                     VPEEDSVCVERLKGAGAIIVGKTTTPEFGHKAVTDSPLFGITRNPWNPALTPGGSSGG
                     AGAAVAAGLGPLAVGTDGGGSIRIPASFCGVYGLKPSFGRVPQFPGFPGWETLSHSGP
                     LTRTVRDAALMLDALAGPDDRDRHSLPAAGRSYLEACEGGIAGLSVAWSPDLGYARLD
                     PEVADLCAAAAERFESFGGHVEVVSPSWEDPEEIFRTMVGAETWGAWGERLEADGEKM
                     DRSLRALMKFGAGVTAEQYLRAMRRRQDFWTDVQRFLARFDLLITPTVAVPPFEVGRP
                     GLNEINGQPSSPLGWTPFCFPFNLTGQPAATVPVGFTSAGLPVGLQIVGRRQADHTVL
                     AASAAFEAAQPWAERRPPVD"
     gene            5515..5904
                     /gene="crcB"
                     /locus_tag="VNU03_09645"
     CDS             5515..5904
                     /gene="crcB"
                     /locus_tag="VNU03_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HWP74769.1"
                     /translation="MTRFALICLGGAFGTGARYVLGGVVTRWAGPEFPYGTLLINVLG
                     SFLIGVVQQVGLSTLLIPETLRLVLAVGVMGGFTTYSSFSYETIRLLEAGSWMAATLY
                     VVLTTALCLACCVAGLSLGRIMVEGRW"
     gene            complement(5915..6130)
                     /locus_tag="VNU03_09650"
     CDS             complement(5915..6130)
                     /locus_tag="VNU03_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74770.1"
                     /translation="MTRSSGPRRVLQGLALVVIVLVAFFAGMLVERLQSHAQRDDMLR
                     RYDRALQEHRSLIMESEKRQEGGTPRR"
BASE COUNT          862 a         2142 c         2331 g          862 t
ORIGIN      
        1 catcaccttg tccacctcgt ggcggagctg caccaccacg ccgggcgtca cttggtgcgt
       61 ctcgaacggg gcggggtgcc agtcgagcgc gccgtcggcg aggaagcgct cgagcggcag
      121 ggcctcggcc gcgtcgtagc ggaagcactc gagcagcgcg gtgaagagct gctcggtcgg
      181 gtagggcccg cggtaccgca cggtggcgac gccgtggtcc ttcatgacgc tcgcgatcag
      241 gttcagcagc gcggtcccgc cgccagccgg caggcggcgc ggctcggcga gcggcgggat
      301 gaagtccggg cgcgcgtact cgaccgcctg gaagcaggtg agcgcctcgc ggggcgccca
      361 cggcgcgtcg ggctcgagct gccacacccg gtcggagagg ccccacgcgg gatgggtccg
      421 ggcggcgggc tcgatgccga gccagcgtcc gtcgggcagg agacacttcg cccactcgag
      481 ccgtccgtcg ggatgccatc gccacgcggc gaccaggtgc ccgcgccggt cgagctggag
      541 caggcgacgg cggccagggc ccggcgcgta tgcggtcagc gtgccgccgc gcccgacggt
      601 caccggcggg tcgggctcgt cgagcgcgag ccggaggcgc tccacgccct ccgggtcgat
      661 ggcggcgagg tcggcgcgcg cgagccagcc ccgcagccgc gcgagcgcgg cgcggtccat
      721 gggcgcgatg atacagtcac ccggacatga gcgtgaccac tcggctcgcc gggcccgacg
      781 acgccgcggc catcgcccgc atctacaacc aggggatcga ggaccgcgtc gcgaccttcg
      841 agaccgagcc gcggaccccg gaggacatcc gggcccagct cgcgagcaag ggcgaccggt
      901 acccgacggt ggtggtcgag cgcgacgggg tggtggtggc gtgggcgtcg gcgggggcat
      961 atcgcgcccg gccggcctac gcgggggtag ccgagcactc ggtctacgtg gaccggagcg
     1021 cgcggggcca cggcgcgggc cgcgccgcgc tcgacgcgct cttccgctgc tacgaggcgc
     1081 gcggcttctg gaagctcgtc tcccgcatct tcccggagaa cgtggcgagc ctcgccctgc
     1141 acgagcgggc cggcttccgc accgtcgggg tgtacgcccg ccacggcaag ctcgcgggcg
     1201 agtggcgcga ctgcgtcatc gtcgagaagc tgctcgggga ggccgcagca gccagcggaa
     1261 gtacgccgtc gccgccgccg cgcccaccag ctgcgccgcg atgaagccgg gggcatcggc
     1321 cggccggatg cccgcgaagg tgtcgctcgc cgcgcgcgcg agggtcaccg ccgggttcgc
     1381 gaacgaggtc gacgaggtga accagtacgc ggcggtgatg taggccgcga ccgcgtacgg
     1441 caccgcggcc gcccggtgac gggcgcagcc ctggatcacc gcgagcaggc cgaaggtcgc
     1501 gaccagctcg ccgaggagct gggccggacc ggcgcggacg tggcgggagg cgaagaagac
     1561 cggctcgccg aacatgaggt gggcgaccgc cacgcccgcg aaggcgcccg cgatctgggc
     1621 gaggacgtag accggcacgt ctcgccacgc gagcccgccg cccgccgctt ccaccagggt
     1681 caccgccggg ttgaagtggg ctccggagat ctcgccgaag gtcaggatga gggcgacgag
     1741 ggccgcgccg gtcgcgagcg tgttggcgag cagggccacc gccacgttgc cgccggccag
     1801 ccgctcgccc atgatgccgg agccgatcac cgcggcgagc aggaaggcgg tccccaccgc
     1861 ttccgcggtc acccgccgcg ggaggtcggg gcgctcgctt ccggacgtca tgccgcgggg
     1921 cggggggcga tctcggcgag ccaggcccgc agccgatcgc ggatcgcgtc gcgaacgcga
     1981 cggaaggtcg cgaggcggtc ggcctcgctg ccggtcgcgg ccgagggatc ctcgaagctc
     2041 cagtggatcc ggcgggccgg gccggggaag agaggacagc gctcgcccgc ctggtcacag
     2101 acggtgatca cgtagtccca ccgctcgccg aggtagcggt cgagcgtctt ggacgagtgc
     2161 ccccggaggt cgagcccgat ctcctccatc acgcgggccg cgagcggatg gaccgcggtg
     2221 gctaccgtgc ccgcgctggc cacctcgaat cggtcgccgc ccagctcacg caggaagcgc
     2281 tccgccatct ggctgcgggc cgagttgtgc gtgcagagga tgagcgcgcg gtcgcgcccg
     2341 ctcgagtggt cagtcacagc ggcgcagtac ccggagtccg cggctagggt gcgtccgaga
     2401 cctttgtggt cggcgccatc gccggcacta tcgatcaact ccggttgatc agtcaagagg
     2461 gcgggtagcc cgaatccggc cccccttcgg gcataactcc tcgacgagcg acgcgggcgc
     2521 gcggtccaac tccccggttt tctgctcccg cggcgcgtgg tatgacgttt gcgttgcagc
     2581 gctgctcttg cggatcaatt tcgtggcgcg ggacttgcgg tgaacgattc acacgacacc
     2641 gggggggcac gatgagccac tatcacgatt cggatgacct gaaggtcctg ggggagttca
     2701 agaagctcgc cccggcggag ttcaaggggt tcgtcgagct cgacagcatc gtcgggcggg
     2761 acgacggcaa gatcccgcgc aagtatcgcg agctgatcgc gctggcggtg gcctgcacca
     2821 cccagtgccc gtactgtctc gacgtccaca cccgcaacgc gaagaaggcg ggcgccaccc
     2881 gcgaagaggt cgcggagtcg gcgctcctgg ccgccgccct gcgcgcgggc gcggcggtga
     2941 cgcacggggc gctcgcggtg aagctgttcg acggggccta ggggaatcga cgccacaact
     3001 tacccggagc tacgacacta ccgagacctt ccttccggtc gcgagcgcag aaattgggga
     3061 atggagcgca cgagaccctc ttcgaggcct ggcacatgcc ttgcggcatg atctggcagg
     3121 cgggcacgat cgcccgttct tgacgagtac ggaaaggagg gtcagacatg aaacggatta
     3181 caggggtggc ggccggtgtg atggccacgt tcttggcggt gggcccgggt ttcgcggcgg
     3241 acgccatgcg ggattcgacg tcgatcgaga agccgggcaa gctcgcgatg cctcaccggg
     3301 tcacgggtag cgtggtcagc gtggacgaga gcgcgcgcac cttcaccgtg caggactcga
     3361 acgggaagga ttacgtgctc accgccgatc cggccctcgg ctcgcagctg gatcaggtga
     3421 aggcgggggg tcacgtcaag gtcagctaca agaagaactc cagcggtcag atgatcgcta
     3481 cgaacagatg atcgctacga agatcgcgcc ggccgatgcg atgaggaccc cgtcgcgata
     3541 gcgcaggctc gtcgcccgga gggggacggc cgatcggggc cgcccctccg ggctcttctt
     3601 tcgcagtgct cgcggcgagc tcgggtttga gccgttgctc gcgggtgccc cagacgttac
     3661 aatcgcgcgc gtgaatcctc gggtcatcgc gatcgggatt gcgctgtgcc tgggggcctg
     3721 cgcgcccgtc gcggtaccga tgccgatcca gccgttcccc gacgtgccgg tgccggccga
     3781 gtggacgccg tactcggacg actgggtgat gattcgctcg ccgaagatca ccgcggcgcg
     3841 gctcatctac ttcactcggg agagcgtcga cgagacgctc gcgcacgcgc ggcggctgct
     3901 ggcccgcgcg gggtggatcg agacccacag cgagcgcttc gtgaacgcgg agaagttttc
     3961 cggggtgtgg gtcgagttcg gcaaggacgc cgacgtctgc cgcctgaccg tgatcgaggg
     4021 caccggggcc acccacgtcg actacaccct cgcgcgcttg aaccccgggt cctgatggcc
     4081 accaccgacc tggtctggcg ctccgcgctc gagctggccg ccctcatccg ggccaagcag
     4141 gtgtcgccgg tggaggtggt cgacgcggtg ctggcccgca tcgagcggct gaacccggcg
     4201 ctcaactgct acgcgaccgt gaccgccgag gaggcgcgcg acgcggcggt ggccgccgag
     4261 gtggcggtgg agaccggcga ggagctcggg ccgctgcacg gcgtgccggt gtccatcaag
     4321 gacctggtgt tcacgcggcg cgtgctcacc accggtggct cgcggctctt caccgatcac
     4381 gtgccggagg aggactcggt ctgcgtcgag cggctcaagg gcgcgggcgc catcatcgtc
     4441 ggcaagacca cgacccccga gttcggccac aaggcggtga ccgacagccc gctcttcggc
     4501 atcacccgca acccgtggaa cccggccctc acgccgggcg gctcgagcgg cggcgcgggc
     4561 gcggcggtgg ccgctggcct gggcccgctc gcggtgggca ccgacggggg cggctccatc
     4621 cgcatccccg cctcgttctg cggcgtctac gggctcaagc cgtccttcgg gcgtgtgccg
     4681 cagttccccg gtttcccggg ctgggagacg ctctcccaca gcgggccgct cacgcggacc
     4741 gtgcgcgacg ccgcgctgat gctcgacgcc ctcgcgggcc ccgacgatcg ggaccggcac
     4801 tcgctgcccg cggccggccg gtcgtatctc gaggcgtgcg agggcggcat cgcggggctg
     4861 agcgtggcgt ggtcgcccga cctgggctac gcgcggctcg atcccgaggt cgccgatctg
     4921 tgcgcggcgg cggccgagcg cttcgagagc ttcggcgggc acgtcgaggt ggtgtcgccc
     4981 tcctgggagg atccggagga gatcttccgg accatggtgg gcgccgagac gtggggcgcg
     5041 tggggcgagc gcctggaggc cgacggcgag aagatggacc gctcgctgcg cgcgctgatg
     5101 aagttcgggg cgggcgtcac cgccgagcaa tacctgcggg cgatgcgccg tcgccaggac
     5161 ttctggaccg acgtgcagcg cttcctggcc cgcttcgacc tcctgatcac gcccacggtg
     5221 gcggtgccgc cgttcgaggt cggccggccc gggctcaacg agatcaacgg ccagccgtcg
     5281 tcgccgctcg ggtggacccc gttctgcttc ccgttcaacc tgaccggcca gccggccgcg
     5341 acggtgccgg tcggcttcac gagcgcgggg ctgccggtgg gcctgcagat cgtcggccgt
     5401 cgtcaggccg accacaccgt gctggccgcg tccgccgcct tcgaggcggc ccagccctgg
     5461 gcggagcgcc gcccgccggt cgactaggcg gcgccccggc gcgccggccc cgacgtgacg
     5521 cgattcgcgc tgatctgtct cggcggagcg ttcggaaccg gcgcccggta cgtcctgggc
     5581 ggtgtcgtga cccgctgggc cgggcccgag tttccctacg ggacgctgct catcaacgtg
     5641 ctcggatcgt ttctcatcgg ggtcgtccag caggtggggc tctccacgct gctgatcccg
     5701 gagacgctgc gcctggtcct cgcggtcggg gtcatggggg gattcaccac ctactcgtcc
     5761 ttctcctacg agaccatccg gctgctcgag gcgggctcct ggatggcggc gaccctctac
     5821 gtcgtcctca ccaccgcgct gtgcctcgcg tgctgcgtgg cgggcctgtc gctcggtcgg
     5881 atcatggtcg agggacggtg gtgagcgggg ccggctagcg tcggggtgtg ccgccctcct
     5941 gccgcttctc cgactccatg atcagcgacc ggtgctcctg cagggcgcgg tcgtagcggc
     6001 gcagcatgtc gtcgcgctgg gcgtgcgact gcaggcgctc cacgagcatg cccgcgaaga
     6061 acgccaccag cacgatcacc acgagggcca ggccctgcag cacccggcgc ggaccggacg
     6121 accgcgtcat gggcgcatga tagcaaggcc gggccgcccc cgcgcttgcg ggcggggtga
     6181 gaatggccag agggcgc
//
LOCUS       DATMCV010000414         3834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_554845, whole genome shotgun sequence.
ACCESSION   DATMCV010000414 DATMCV010000000
VERSION     DATMCV010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554845"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VNU03_09655"
     CDS             complement(<1..541)
                     /locus_tag="VNU03_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74771.1"
                     /translation="MLHWIKRIGSTLLALAFTLTTGTAYAQGTIKVMYTDPLSGPFAQ
                     VGDQNLQQFKYIIDYINGRGGALGKKFELVSFDNKSQPSEALLALKSATDQNIPVIMQ
                     CSGSNIAAALIEGVDKHNERNPTNRIVYVNCGAVATELTNEKCSFWHFRMDAHVGMKA
                     ETMVRALPKDVTKVYLLNQD"
     gene            1011..1502
                     /locus_tag="VNU03_09660"
                     /pseudo
     CDS             1011..1502
                     /locus_tag="VNU03_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(1540..2640)
                     /locus_tag="VNU03_09665"
     CDS             complement(1540..2640)
                     /locus_tag="VNU03_09665"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWP74772.1"
                     /translation="MEARLVDEIPAGPGWQYEPKWDGFRCLAFRDGDRIELQSKSGQP
                     LARYFPEVVEHLRSLPARSFVLDGEIVVPVDGRLSFDDLLQRIHPAASRIARLSQERP
                     AVLIVFDLLVDAAGTSLVDRPLRDRRRMLETFAAKALAGRDDLRLSPATEDTGQARAW
                     LDMTGGTLDGIVAKRLDLPYQTGERTGMQKVKRRRTVDCVVGGFRYATGARVVGSLLL
                     GLYDADGLLHHVGFTSGFATAERKALLTRLAPLRKPPGFTGRAPGGPSRWSTERSGEW
                     EPLEPRLVVEVSYDHFTGDRFRHATRFLRWRPDKAPRQCTLEQVAASSRAPDPPGSAS
                     APRPGSGGGSRRRAPSTSRRSSAGSRRRAARG"
     gene            complement(2670..3647)
                     /gene="ligD"
                     /locus_tag="VNU03_09670"
     CDS             complement(2670..3647)
                     /gene="ligD"
                     /locus_tag="VNU03_09670"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWP74773.1"
                     /translation="MSPRPAARRRAPRAAAASPARLLPRDTNTVEARVGGHAVTLTNL
                     RKPFWPEEGRTKRDLLQYYFDLAPVLLPHLKDRAMVMKRYPNGADGPFFFMKRAPSPR
                     PDWIETCAIEHASKSVIDFPVIQDAAALLWVVNLGCIDLNPWYARCDDVDRPDFLHFD
                     LDPVKGAGFEEVRQTALVLRDALDGLKMPSYPKSTGSRGIHVYVPIVRGPTQKEVWGV
                     SKAIAQALAIARPGLITAEYRIAKRPLGRVLVDYNQNAWGRTLASVYSVRPRPRAPVS
                     APVTWREVERGFQIDDFRVDNMAARVAKQGDLWKPLLARTGRFRLERLR"
     gene            complement(3644..>3834)
                     /locus_tag="VNU03_09675"
     CDS             complement(3644..>3834)
                     /locus_tag="VNU03_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74774.1"
                     /translation="ALGDRLRFPADFVGRGDMSRGGLLLRCVRTGRELAYVPVTGAVR
                     RGGRPARLEHLPRLERLP"
BASE COUNT          533 a         1314 c         1437 g          550 t
ORIGIN      
        1 agtcctggtt gagcaggtac accttggtca cgtccttggg cagggcgcgc accatggtct
       61 cggccttcat gcccacgtgg gcgtccatgc ggaagtgcca gaagctgcac ttctcgttgg
      121 tcagctcggt ggccaccgcc ccgcagttca cgtagacgat acggttggtc gggttgcgct
      181 cgttgtgctt gtcgacgccc tcgatcaggg cggccgcgat gttggagccc gagcactgca
      241 tgatgaccgg gatgttctgg tcggtggccg acttcagcgc gagcagggcc tcggacggct
      301 gcgacttgtt gtcgaacgag accagctcga acttcttgcc gagggccccg ccgcggccgt
      361 tgatgtagtc gatgatgtac ttgaactgct gcaggttctg atcgccgacc tgggcgaagg
      421 ggcccgagag cgggtcggtg tacatgacct tgatcgtgcc ctgcgcgtag gcggttccgg
      481 tggtgagggt gaaggccagg gccaggagcg tcgagccgat ccgcttgatc cagtgaagca
      541 tggggcctcc tccttgagtg gcgcggcgcg ccttcatccg gggtctgagc agggcgaggg
      601 tatccgagtg cgccaggcaa gtcaaagggg gcgggggctc gcccgcggag cggggagagg
      661 ctaggatggc ggccatgctc aacgtgctcg tgaccatgcg cttcgaccag gcccagctcg
      721 accggctgcg ggcggtctcc cccgagatct cggcgtccca ggccgatccg gccaccgccg
      781 actacgccaa gacccacgtc ctctacgcgg ggacgccccc gcgtgatctg tcgcgcgccc
      841 ccgcgctgcg atgggtgcag ctgcacatgg cgggggtgaa cgccctcgcc gaccacccgc
      901 tctacacgga gaccgcgctg ccgctcacca ccaccagcgg cgtgcacgcg gcgaccatcg
      961 ccgagtacgc ggtgacgatg ctgctggccc tggcccaccg ggtgccgcgc atggtcgagt
     1021 ggcaggggca cggcggctgg ccacccgacg agcagcgctg gccgctcttc gtgccgtcgg
     1081 aggtgcgggg ggcgacactc ggcatcatcg gctacggcag catcgggcgc gagctcgcgc
     1141 gcatcgccaa gtctgccttc ggcatgacgg tgctggccgg caagcgggat ccgtcacggc
     1201 gtgtcgacga cggctactgc ctgcccggga ccggcgatcc ggagggccgg ctgcccgacc
     1261 aatggctggg catggccgag atcgacacgc tgctggcgcg ctcggacgtg gtcgtgctct
     1321 gcgcgccgct gaccgcggag acccgcggtc tgatcgggga ggccgcgctg cggcgcatga
     1381 agccgtcggc ctacttcatc aacgtggggc ggggcgcgag cgtcgacgag gccgcgctga
     1441 cccgtgcgct gcgcgagcgg tggatcgcgg gcgcggccct cgacgtcttc gcccaggagc
     1501 cgccgcccgc ggggcatccg ttctacggcc tcgacaacgt catcctctcg ccgcacgtct
     1561 ccggcttcct gccgagctac gacgagaagt gctcggtgct cttcgccgag aacctccgcc
     1621 gctacctggc cggggagccg atgctgaacc tggtggatcg ggcgcgcggc tactagccgc
     1681 gacctgctcg agcgtgcact gccgcggcgc cttgtcgggc cgccagcgga ggaagcgggt
     1741 ggcgtgccgg aagcggtcgc ccgtgaagtg gtcgtagctg acctcgacga ccagccgcgg
     1801 ctcgagcggc tcccactcgc cggagcgctc cgtgctccag cggctgggcc cgcccggcgc
     1861 gcgcccggtg aagccgggcg gcttgcgcag gggcgcgagc cgggtgagga gcgccttgcg
     1921 ctccgcggtt gcgaagccgg aggtgaagcc cacatggtgg agcagcccgt cggcgtcgta
     1981 gaggccgagc aggagcgagc ccaccacccg ggcccccgtg gcgtaccgga atcccccgac
     2041 cacgcagtcg acggtgcgcc ggcgcttgac cttctgcatg ccggtgcgct cgccggtctg
     2101 gtagggcagg tcgaggcgct tggccacgat gccgtcgagc gtgccgcccg tcatgtcgag
     2161 ccaggcgcgc gcctggccgg tgtcctcggt ggccggagaa aggcggaggt cgtcgcggcc
     2221 ggcgagcgct ttggccgcga aggtctcgag catccgccgg cggtctcgga gcggccggtc
     2281 gacgagcgat gtcccggccg cgtccaccag caggtcgaag acgatgagca ccgccggccg
     2341 ctcctgggag aggcgcgcga tgcggctcgc ggccgggtgg atgcgctgga ggaggtcgtc
     2401 gaaggagagc cgcccgtcga ccggaaccac gatctcgccg tcgagcacga acgagcgggc
     2461 gggcagcgag cgcaggtgct ccaccacctc cgggaagtag cgggcgagcg gctggccgct
     2521 cttggactgc agctcgatgc gatcgccgtc gcggaaggcg aggcagcgga agccatccca
     2581 cttgggctcg tactgccagc ccgggccggc cgggatctcg tcgacgagcc gcgcctccat
     2641 cggcgcgtag ggcggccgca gcggcaggct caccgcaggc gctcgaggcg gaagcggccg
     2701 gtccgggcca ggagcggctt ccagaggtcg ccctgcttcg ccacgcgcgc ggccatgttg
     2761 tcgacgcgga aatcgtcgat ctggaagccg cgctccacct cgcgccaggt caccggggcc
     2821 gacaccgggg cccgcggccg gggccgcacc gagtacaccg aggcgagcgt gcgcccccac
     2881 gcgttctggt tgtagtccac cagcacgcgg ccgagcggcc gcttggcgat ccggtactcg
     2941 gcggtgatca ggccgggccg cgcgatggcc agggcctgcg cgatggcctt cgacacgccc
     3001 cagacctcct tctgggtcgg gccacgcacg atcggcacgt acacgtggat cccgcgcgag
     3061 ccggtggact tggggtagct cggcatcttc aggccgtcga gcgcgtcgcg cagcaccagg
     3121 gcggtctgcc gcacctcctc gaagccggcg cccttgaccg ggtcgagatc gaagtgcagg
     3181 aagtcggggc gatcgacgtc gtcgcagcgc gcgtaccagg gattcaggtc gatgcacccg
     3241 aggttgacca cccacaggag cgcggcggcg tcttggatca ccgggaagtc gatcacgctc
     3301 ttcgacgcgt gctcgatcgc gcaggtctcg atccagtccg ggcggggaga cggggcgcgc
     3361 ttcatgaaga agaacgggcc gtctgccccg ttcgggtacc gcttcatgac catcgcgcga
     3421 tccttcagat ggggcaggag caccggcgcc aggtcgaagt agtactggag gaggtctctc
     3481 ttggtgcggc cctcctcggg ccagaacggc ttgcgcaggt tggtgagcgt gaccgcgtgg
     3541 cccccgaccc gagcctcgac ggtattcgta tcgcgcggga gcaggcgagc gggggacgcg
     3601 gcggccgcgc gcggggcccg gcgcctggcg gccggccgcg ggctcatggc agccgctcca
     3661 gccggggcag gtgctcgagg cgggcgggtc ggccgccgcg gcgcaccgcg ccggtcaccg
     3721 gcacgtaggc gagctcgcgc cccgtccgca cgcagcggag cagcagcccg ccgcggctca
     3781 tgtcgcctcg accgacgaag tcggccggga agcggagccg atccccgagc gcct
//
LOCUS       DATMCV010000415         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_889025, whole genome shotgun sequence.
ACCESSION   DATMCV010000415 DATMCV010000000
VERSION     DATMCV010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889025"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VNU03_09680"
     CDS             <1..276
                     /locus_tag="VNU03_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP74775.1"
                     /translation="RLAPEKIRVNAVCPGPIDTPMLRVFVARPDQKSTAGMDPEDLVR
                     RRAAGSVPLGRTGRPEEIANAALFLLSDEASFITGAALPVDGGVTAA"
     gene            273..1529
                     /locus_tag="VNU03_09685"
     CDS             273..1529
                     /locus_tag="VNU03_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74776.1"
                     /translation="MSERRRTRSTPRARLAVVALVLAVLATCPAAIAPASAAGFLEGT
                     HEGADFLIGVPARWNGGLVLFAHGYEGEGDGRGSVRASPLSSHLEARGYAWAASGFRS
                     RGYRPDWFLADMIALRSRFIAQFGVPRWTILHGQSMGGHVTIASLELHPEIYQGGLIE
                     CGIVDGVSLVDWLYAYTAAAEYVSGVRLLDAPDRQAFSALVNGPWLGAVGTPGSYTER
                     GRRFDSVVKHLMGGELPLRLEGMRQRYIMNLNSRDPGPDRAREFARHASTTHIRYDID
                     PGLGLTADELNRGIRRITPAPGARSREGNPVFAELTGKIRVPLMTIHETADFRVPFRL
                     QQDYRRRTLAAGTSHLLVQRAVRWAGHCAFDGTVRERAFDDLVEWIERGVVPAGDDVL
                     APDVSRLGLRWTPALHPADPLAPAGR"
     gene            1564..2028
                     /locus_tag="VNU03_09690"
     CDS             1564..2028
                     /locus_tag="VNU03_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWP74777.1"
                     /translation="MPTSKPLSPQIEQFIHDNPQGVLTSFRRNGMPQLSIVTVYPRDG
                     GVGISITETRMKFKNFLRDPRCSVLISHADWWSGFLVFEGKAELTHSGNTDPDALRLA
                     RRHIFSATTRRRSRDWEEYDRITEADKRVAMVLRPEHVYGSALAKMRDALAK"
     gene            complement(2056..>2801)
                     /locus_tag="VNU03_09695"
     CDS             complement(2056..>2801)
                     /locus_tag="VNU03_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP74778.1"
                     /translation="LRLLPLAEQKRRLADPTTRRRLVAEEAAMKPRDNVFQGGGAATT
                     DPRRPDYDNLYAMKDVEWNDPTVAQLAAARGQHPVDVMIDLALANDNQVFVQPLVNEH
                     PDHVLGMLKHPHTLATFSDSGAHVCQEMGSSLQTHMLSYWVRAKGAFTLEQAVRKLSF
                     DNAAAWGLDDRGLLKQGYRADLVLFDEARVRPAMPTVEADLPGGARRLVQKAEGIAAT
                     IVNGEVTLENGQSTGRLPGVLLRGPAAGR"
BASE COUNT          384 a         1060 c          950 g          407 t
ORIGIN      
        1 cgcctggccc ccgagaagat ccgagtcaac gcggtgtgcc cgggccccat cgacacgccg
       61 atgctgcgcg tcttcgtcgc gcgccccgac cagaagtcga ccgcgggcat ggatccggaa
      121 gatctcgtcc gccgccgcgc cgccggatcc gtgccgctcg gccgcaccgg ccggccggag
      181 gagatcgcca acgccgcgct cttcctgctc tccgacgagg cgtcgttcat cacgggcgcg
      241 gccctgccgg tcgatggagg cgtgaccgcg gcgtgagcga gcgacggaga acccggtcca
      301 ccccgcgggc ccgcctggcg gtcgtcgcac tcgtactcgc agttctcgcg acgtgcccgg
      361 ccgcgatcgc cccggcatcc gcggccggct tcctcgaagg cacccacgag ggcgccgact
      421 tcctgatcgg cgtccccgcg cgctggaacg gcggcctggt cctgttcgcc cacggctacg
      481 aaggcgaggg cgacggccgc gggagcgtgc gcgcctcgcc gctcagcagc catctcgagg
      541 cgcgcggcta cgcttgggcc gcgtccggct ttcgcagccg cggctatcgg cccgactggt
      601 tcctggccga catgatcgcg ctgcgctccc gcttcatcgc ccagttcggc gtcccccgat
      661 ggaccatcct ccacggccag tcgatgggcg gccacgtcac gatcgcatcg ctcgagctcc
      721 atccggagat ctaccagggg ggtctgatcg agtgcggcat cgtggatggg gtgagcctgg
      781 tggactggct ctacgcctac accgcggccg cggagtacgt ctccggagtc cgtctcctcg
      841 acgccccgga ccgccaggcg ttcagcgcgc tcgtcaacgg tccctggctc ggtgcggtgg
      901 gcacgcccgg gagctatacc gagcgcgggc gccggttcga cagcgtggtg aagcacctga
      961 tgggcggcga gctgccgctc cgcctcgagg gcatgcggca gcgctacatc atgaacctca
     1021 actcccgcga tccggggccc gaccgcgcgc gggagttcgc ccggcacgcg agcacgacgc
     1081 acatccgcta cgacatcgac cccggcctgg gcctgaccgc ggacgagctg aaccgcggga
     1141 tccgccggat cacccccgcc ccgggcgcgc gatcccgcga gggcaacccg gtgttcgcgg
     1201 agctgaccgg caagatccgc gtgcccctga tgacgatcca cgagacggcg gacttccgcg
     1261 tcccctttcg cctgcagcag gactatcgcc gacggacact cgccgcggga acctcgcacc
     1321 tcctggtgca gcgggcggtg cgctgggccg ggcactgcgc cttcgacggt acggtgcgcg
     1381 agcgcgcgtt cgacgatctg gtcgagtgga tcgagcgggg cgtggttccg gcgggcgacg
     1441 acgtgctcgc gcccgacgtc tcgaggctcg gcctgcgctg gacgccggct ctccaccccg
     1501 cggatcccct ggccccggcc ggacgctgag cgcgtctcca tcccctcacg aaggagcgca
     1561 ccgatgccga cgtccaagcc cctgtcgccg cagatcgagc agttcatcca cgacaatcca
     1621 cagggcgtgc tgacgtcctt ccggcgcaac ggcatgcccc agctcagcat cgtcaccgtc
     1681 tatccacgcg acggcggcgt cgggatctcc atcaccgaga cccgcatgaa gttcaagaac
     1741 ttcctgcgcg acccacgctg ctcggtgctg atctcccacg ccgactggtg gtcgggcttc
     1801 ctggtcttcg agggcaaggc ggagctcacc cactccggca acaccgaccc cgacgcgctc
     1861 cgcctggccc gccgccacat cttcagcgcc accacccggc gccggagccg cgactgggag
     1921 gagtacgacc gcatcaccga ggcggacaag cgcgtggcga tggtgctgcg tcccgagcac
     1981 gtgtacggct ccgcgctggc caagatgcgc gacgccctgg ccaagtagcg ccgccgcggc
     2041 gcgcgccggc tcccgtcagc gccccgcggc cggaccccgc agcaggacgc cgggcagccg
     2101 gccggtcgac tggccattct cgagcgtgac ctcaccgttg acgatggtgg ccgcgatgcc
     2161 ctccgccttc tgcaccaggc ggcgggcgcc gccgggcaga tcggcctcga ccgtcggcat
     2221 cgccggcctg acgcgcgcct cgtcgaacag caccaggtcg gcccggtaac cctgcttcag
     2281 cagcccgcga tcgtcgagcc cccaggcggc cgcgttgtcg aagctcagct tgcgcaccgc
     2341 ctgctcgagc gtgaacgcgc ccttcgcgcg cacccagtag ctcagcatgt gggtctgcag
     2401 cgacgagccc atctcctggc agacgtgggc gcccgagtcc gagaaggtcg cgagggtgtg
     2461 cgggtgcttg agcatgccga gcacgtgatc cgggtgctcg ttgacgagcg gctgcacgaa
     2521 tacctggttg tcgttggcga gcgccagatc gatcatcacg tcgacgggat gctggccccg
     2581 cgcggccgcg agctgggcca ccgtggggtc gttccactcc acgtccttca tcgcgtagag
     2641 gttgtcgtag tccggccggc gcgggtcggt ggtggccgcc ccgccaccct ggaacacgtt
     2701 gtcgcggggc ttcatcgccg cctcctccgc caccagccgc cgacgggtgg tcggatcggc
     2761 gaggcgccgc ttctgctctg cgagcggcag gagccgcagc t
//
LOCUS       DATMCV010000416         2013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_958411, whole genome shotgun sequence.
ACCESSION   DATMCV010000416 DATMCV010000000
VERSION     DATMCV010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958411"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VNU03_09700"
     CDS             complement(<1..475)
                     /locus_tag="VNU03_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP74779.1"
                     /translation="MISETFILNQVTGLIDLGHEVDIFARRPSADRAVHPEVERYGLL
                     ARTHWFDLPRTRWGRVKRATAAFLRAFPRHPLALLRCLNLPRYGSFYAVLNNVMHILP
                     FLEGRYDVVMCHYGGNGAEFIFLKDLLPGLKFVTMFHKGDIQLGDERTPAVYRHLP"
     gene            complement(616..>2013)
                     /locus_tag="VNU03_09705"
     CDS             complement(616..>2013)
                     /locus_tag="VNU03_09705"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP74780.1"
                     /translation="DDEPGVRESLRLILGDEFEVLEAADGAAALAILGGRRVDVALLD
                     VRMPGEPGSRVLPQILAIDEAIPVILITADPHLRTAVDAMKAGAYDYLAKPFDVDEIL
                     GLVRQAAQQRGMERELHYLRAELDQAHGFDQLVGRHPRMVRLYEVIAQVSQTHATVLI
                     TAESGTGKELVARAIHNQSPRRAQPFVAVNLAAIPDSLLESELFGHEKGAFTGAYARK
                     PGKFELAHGGTLFLDEVGSLRVDLQAKLLRALQQREIERLGGTRTIQVDVRVIAATNS
                     DLRQAIRARTFREDLYYRLHVVPIGVPPLRERKSDLPDLAAHFVRKYAREFKKDVRAL
                     SRGARVALDGYDWPGNVRELENIIERGVALATRPVIGLDDLPLELAMNEAAPAHGEAE
                     PGPLTLREARDRFEQAYVLRALEREGWNQSRAARGLGVHRNTLLARLATWGFRRDEPA
                     TGSRPAMGGEPTARA"
BASE COUNT          298 a          690 c          728 g          297 t
ORIGIN      
        1 ccggcaggtg gcggtagacg gcgggtgtcc gctcgtcacc cagctggatg tcacccttgt
       61 ggaacatcgt cacgaacttc agtcccggca ggaggtcctt gaggaagatg aactcggcgc
      121 cgttgccccc gtagtggcac atgacgacgt cgtagcgccc ctcgaggaac ggcaaaatgt
      181 gcatgacatt gttgaggacc gcgtagaaac ttccgtagcg gggaaggttc aagcagcgta
      241 acagcgcgag cggatgccgg ggaaacgccc gcaggaacgc ggcggtcgcg cgtttcaccc
      301 gtccccagcg ggttctcggc aggtcaaacc agtgggtccg cgcgagcaga ccgtagcgct
      361 ccacttccgg atgcaccgcc cgatccgcgc tgggccggcg cgcgaagatg tcgacctcgt
      421 ggcccaggtc gatcaacccg gtaacttggt tcagtatgaa cgtctcgctg atcatcggga
      481 agccgccgac tatgaaagct atgcgcacgt cggctcgctc cgctgatccg cgctcgggga
      541 gggcatggag ctacgctacg cgaagatcgg ttacgcggct actaagttgc gggagcggcg
      601 gagcgggaga tcgggtcagg cgcgagcggt cggctcgccg cccatcgcgg gccgcgagcc
      661 ggtcgccggc tcgtcgcggc ggaagcccca cgtggcgagg cgcgcgagga gcgtattgcg
      721 atgcacgccg agcccgcgcg cggcccggct ctggttccag ccctcccgct cgagcgcgcg
      781 cagcacgtag gcctgctcga agcggtcgcg cgcctcgcgc agggtgagcg gaccgggctc
      841 cgcctcgccg tgcgcgggcg ccgcctcgtt catggccagc tccagcggca ggtcgtcgag
      901 gccgatcacc gggcgcgtgg ccagcgccac gccgcgctcg atgatgttct cgagctcgcg
      961 cacgttgccc ggccagtcgt agccgtcgag cgccacgcgc gccccgcggg agagcgcgcg
     1021 cacgtccttc ttgaactcgc gggcatactt gcgcacgaag tgggcggcca gatccggcag
     1081 gtccgacttg cgctcccgca gcgggggcac cccgatgggc accacgtgga gacggtagta
     1141 gaggtcctcg cggaacgtgc gcgcccggat cgcctgccgc aggtcgctgt tggtggccgc
     1201 gatcacccgc acgtccacct ggatggtgcg cgtgccgccg aggcgctcga tctcccgctg
     1261 ctgcagggcg cggaggagct tcgcctgcag gtcgacgcga aggctgccga cctcgtcgag
     1321 gaagagggtg cccccgtggg ccagctcgaa cttgcccggc ttgcgcgcat acgcgccggt
     1381 gaaggcgccc ttctcgtggc cgaacagctc ggactcgagc agggaatccg ggatcgcggc
     1441 caggttgacc gccacgaaag gctgcgcgcg ccgcgggctc tggttgtgga tcgcgcgggc
     1501 gacgagctcc ttgccggtgc cgctctccgc ggtgatcagc acggtcgcgt gagtctgcga
     1561 cacctgggcg atcacctcgt agagccggac catccgcggg tgccgtccca cgagctggtc
     1621 gaagccgtgg gcctgatcga gctcggcccg caggtagtgc agctcgcgct ccatgccgcg
     1681 ctgctgggcg gcctggcgga ccaggcccag gatctcgtcc acgtcgaagg gcttggcgag
     1741 gtagtcgtag gcgcccgcct tcatggcgtc caccgccgtg cgcaggtgag gatccgcggt
     1801 gatcaggatg accgggatcg cctcgtcgat ggccaggatc tgcggcagca cgcgggagcc
     1861 gggctccccg ggcatccgca cgtctagcag ggccacgtcg acccgccggc cgcccaggat
     1921 cgcgagggcg gcggcgccgt cggcggcctc cagcacctcg aactcgtcgc cgaggatcag
     1981 ccgcagcgac tcgcggaccc cgggctcgtc gtc
//
LOCUS       DATMCV010000417         2206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_91102, whole genome shotgun sequence.
ACCESSION   DATMCV010000417 DATMCV010000000
VERSION     DATMCV010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91102"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VNU03_09710"
     CDS             <1..785
                     /locus_tag="VNU03_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74781.1"
                     /translation="IALQYLRWVGRALRGDLGESIRTRESVVWTVARKLPITMELAGL
                     SLLIALGIAIPSGIFSAVRRNTVWDFLVNAVSLCGLSVPSFWLGLMLILLLSVRLHWL
                     PASGFVPPWEDLGANLRRMLMPAFVLGAGLAAVLMRQTRNSMIEILSADYIRTAWSKG
                     LAGRAVIFRHAFRNGLIPVVTILGLQTGALMGGAVVTESIFVVPGFGRLIVESVFTRD
                     YPLVQGVVLITASTYVLVNLLVDLSYSLLNPRIRIGGAASGG"
     gene            775..1620
                     /locus_tag="VNU03_09715"
     CDS             775..1620
                     /locus_tag="VNU03_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74782.1"
                     /translation="MAAERGGRLWRRLWRRPPAVVGALVVLFFVALAVGAPWIATSDP
                     QRSDYGKIRKAPTAANPFGTDDLGRDNFSRVVWGTRISMQAGVFSIVLAIAIGVPAGL
                     VAGYWSGGVIDQLIMRLTDAWLAFPFLILAIGLVTIMGASLTNATVAIGLAATPTFIR
                     LTRGLVLGTREEEYVQAARALGAGGPRTILRHILPNIVSALLVQATVSIPAAIIAEAI
                     LSFLGLGVQPPTPSWGTMLNTAQQFLETAPWMAWWPGLAIFSLTLSFNLAGDGVRDLL
                     DPKDY"
     gene            complement(1673..>2206)
                     /locus_tag="VNU03_09720"
     CDS             complement(1673..>2206)
                     /locus_tag="VNU03_09720"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWP74783.1"
                     /translation="ALDALLAMTRAGEVRADEVERVVVTTIPFGPRMAGTAPASMLGA
                     KFSIPYAVAAALVLGRADVSAFVEPALGDPRIRALAGRVEVRVDPEMSPRRADAPTAR
                     VEVGLKDGRALTGVTSVVRGDFEDPVPTEEVVEKFVALASDVLDVDRARAVVQIVDRV
                     DTLKNLHDLTELLAPAT"
BASE COUNT          274 a          815 c          763 g          354 t
ORIGIN      
        1 cgatcgccct gcagtacctg cgctgggtcg ggcgcgcgct gcggggcgac ctcggggagt
       61 cgatccgcac ccgcgagtcg gtggtatgga cggtggcccg caagctgccg atcacgatgg
      121 agctggccgg cctgtcgctc ctgatcgcgc tcggcatcgc gatcccctcc ggcatcttct
      181 ccgcggtccg ccgcaacacg gtgtgggatt tcctcgtcaa cgcggtctcg ctctgcggcc
      241 tctcggtgcc gagcttctgg ctcgggctca tgctgatcct gctcctgtcg gtgcggctgc
      301 actggctccc cgcctccggc ttcgtgccgc cgtgggagga cctgggggcc aacctccggc
      361 gcatgctgat gcccgcgttc gtgctggggg ccgggctcgc ggcggtgctg atgcgccaga
      421 cccggaacag catgatcgag atcctgagcg cggactacat ccgcaccgcc tggagcaagg
      481 ggctggccgg ccgcgcggtc atcttccgcc acgccttccg caacgggctg atcccggtgg
      541 tcaccatcct gggcctccag accggcgccc tcatgggcgg cgcggtggtc accgaatcga
      601 tcttcgtggt acccggcttc gggcgactga tcgtggagtc ggtgttcacc cgcgactacc
      661 ccctcgtgca gggcgtggtg ctgatcaccg ccagcaccta cgtcctggtc aacctgctgg
      721 tggacctctc ctactccctg ctgaacccgc gcatccgcat cggaggggcc gcgagtggcg
      781 gctgagcggg gcgggcgcct ctggcggcgg ctgtggcggc gcccgcccgc ggtggtgggc
      841 gcgctggtcg tgctcttctt cgtggcgctc gcggtcgggg ccccgtggat cgcgacgtcg
      901 gatccgcagc gctccgacta cggcaagatc cgcaaagcgc cgaccgccgc gaacccgttc
      961 ggcaccgacg acctcggccg ggacaacttc tcccgcgtgg tctggggcac ccgcatctcg
     1021 atgcaggcgg gggtgttctc gatcgtgctc gcgatcgcca tcggggtgcc ggcggggctg
     1081 gtggccgggt actggagcgg cggcgtcatc gaccagctca tcatgcggct gaccgacgcc
     1141 tggctcgcct tcccgttcct gatcctggcg atcggcctcg tgacgatcat gggggcctcg
     1201 ctcaccaacg cgacggtggc catcggcctc gcggccacgc ccaccttcat ccggctcacc
     1261 cgggggctcg tgctcgggac gcgcgaggag gagtacgtgc aggcggcgcg cgcgctcggc
     1321 gcgggcgggc cgcggacgat cctccgccac atcctgccca acatcgtgag cgccctgctc
     1381 gtgcaggcca ccgtgtcgat cccggccgcc atcatcgcgg aggccattct ctcgttcctg
     1441 ggtctgggcg tgcagccgcc cacgccgagc tggggcacca tgctcaacac cgcccagcag
     1501 ttcctcgaga ccgcgccgtg gatggcctgg tggcccggcc tcgcgatctt ctcgctgacg
     1561 ctgtcgttca acctggccgg cgacggcgtc cgcgacctgc tcgaccccaa ggactactag
     1621 cctctccctc tcccctccgg ggagagggca gggtgagggg tgatgcgctt cgctacgtcg
     1681 ccggcgccag aagctcggtc aagtcgtgca ggttcttgag tgtgtcgaca cggtccacga
     1741 tctgcaccac cgcgcgagcc cgatcgacgt caagaacatc cgacgccagc gcgacgaatt
     1801 tctccacgac ttcctcggtc ggcaccggat cctcgaagtc gccgcgcacc acgctggtga
     1861 ccccggtgag cgcgcgccca tccttgagcc cgacctcgac ccgcgcggtc ggggcgtcgg
     1921 cgcgccgggg cgacatctcc gggtccaccc gcacctcgac gcgacccgcg agggcccgga
     1981 tgcgcgggtc gccgagcgcg ggctccacga aggccgagac gtcggcgcgg ccgaggacga
     2041 gcgcggccgc caccgcgtac gggatggaga acttggcgcc cagcatcgag gccggcgcgg
     2101 tgccggccat ccgcgggccg aacgggatcg tggtgacgac cacgcgctcg acctcgtcgg
     2161 cccgcacctc cccggcgcgc gtcatcgcga gcagcgcgtc cagcgc
//
LOCUS       DATMCV010000418         2954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_158923, whole genome shotgun sequence.
ACCESSION   DATMCV010000418 DATMCV010000000
VERSION     DATMCV010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158923"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VNU03_09725"
     CDS             complement(<1..1197)
                     /locus_tag="VNU03_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWP74784.1"
                     /translation="MADDSPPPSASSADATEEWRPRTSPVLIAISVMLGTFMEVLDTS
                     VANVALPHIAGSLSATPEQATWVLTSYLISNAIVLPVTAWLSAVFGRKRFLLVCIVIF
                     TLASAACGAATSLELLILARVVQGIGGGALQPIAQAILLESFAPARRGRAMAAYSMGV
                     IVAPILGPTLGGWITDSYSWRWIFYINLPVGLAAVIMTQAFIEDPPYLQRTVLRRMDY
                     WGLGFMALWLATLQVVLDKGQQVDWLAAEWIRWTLTISCGAMVAFIVRELVTDEPIVN
                     LRILGDRNFATGTVLITAMGVVLYGTTALLPLFLQTLLGYPALEAGLATSPRGLGALL
                     SAIVVGRLVGVLDARLLVAVGFGFLALSGVWLSRLTFDVAWSNIAFVTFLNGLANPLI
                     FIALSTM"
     gene            1245..2567
                     /locus_tag="VNU03_09730"
     CDS             1245..2567
                     /locus_tag="VNU03_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWP74785.1"
                     /translation="MTDLDQEGISGRLRARFVTYQEAPPRRRYALLGLTLLALVAIGY
                     GIYTWYYWRHHISTDDAYITAHIAPMSARVPGIVVEVAVNDNQDVKPDQVLLRLDPRD
                     YEVAVAQARAAVVSAKGDLQNAVVSVPLIDDSTRSLVQQADAALGATQHGTEMARQDL
                     EQRHAEIQSRLAAVAAAEAAVRSAQADYDRAKLDRDRTVALVKSLLVAQQEMDHAEAT
                     YRTTEAALDAARKRLDQARSDVFQAQASQRSQSAAVAQSVRRVDESRASLENARSQRQ
                     QVGVRQAQVEAAQGRLAQAIANLQQAELNLVYCTIRAPMAGRVSRKSVELGQVVTAGQ
                     ALLAVVDLDDVWVLANYKETELTDVRPGQSATVTVDTYPGKKFRARVDSIQAGSGAVF
                     SLLPPENATGNFVKVVQRVPVKLLFEPGENAQHLLVPGMSVVPIISIR"
     gene            2626..>2954
                     /locus_tag="VNU03_09735"
     CDS             2626..>2954
                     /locus_tag="VNU03_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP74786.1"
                     /translation="MKIDVLLIPVGARYPDMRAAALAAEESGFDGVWTWDHLRDPDEA
                     AESGVPEAWTVLTALAEATRRITLGPLVLNTGLRHPGLLANMAATLQQVSGGRLLLGI
                     GAGATPS"
BASE COUNT          476 a         1012 c         1080 g          386 t
ORIGIN      
        1 catggtggag agcgcgatga agatgagcgg gttggcgagc ccgttcagga aggtgacgaa
       61 cgcgatgttc gaccacgcca cgtcgaaggt gaggcgcgag agccagactc cggacagcgc
      121 gaggaagccg aagccgaccg cgaccagcag gcgcgcgtcc aggaccccga cgagtcggcc
      181 caccacgatg gcggagagca gcgcgcccag gccccgcggg ctggtggcga gcccggcctc
      241 gagcgcgggg tagccgagca gggtctggag gaagagcggg agcagggcgg tcgtcccgta
      301 gaggacgacg cccatcgcgg tgatgagcac ggtgccggtc gcgaagttgc gatcgcccag
      361 gatgcgcagg ttgacgatcg gctcgtcggt gacgagctcg cggacgatga aggccaccat
      421 cgccccgcag gagatggtga gcgtccagcg gatccactcc gccgcgagcc agtcgacctg
      481 ctggcccttg tcgagcacca cctgcagggt ggcgagccag agggccatga atccgagccc
      541 ccagtagtcc atccgccgca ggacggtgcg ctggagatac ggcgggtcct cgatgaaggc
      601 ctgggtcatg atgaccgcgg ccaggcccac cggcaggttg atgtagaaga tccaccgcca
      661 cgagtagctg tcggtgatcc agccgccgag ggtcgggccc aggatcgggg ccacgatcac
      721 tcccatcgag taggccgcca tcgcgcggcc gcgccgggct ggcgcgaagc tctcgagcag
      781 gatcgcctgc gcgatcggct gcagcgcccc gccgccgatc ccctgcacga cgcgggcgag
      841 gatcagcagc tcgagactgg tggccgcgcc gcacgccgcg gaggcgaggg tgaagatcac
      901 gatgcacacc aggaggaagc gcttgcggcc gaagaccgcg ctgagccacg cggtcaccgg
      961 gagcacgatc gcgttggaga tgaggtagct ggtgagcacc cacgtcgcct gctccggggt
     1021 ggcggagagg ctgcccgcga tgtgggggag cgccacgttg gcgaccgagg tgtcgagcac
     1081 ctccatgaag gtgccgagca tcaccgagat cgcgatgagg accgggctcg tccgcggccg
     1141 ccactcctcc gtggcgtccg cggacgaggc gctcggcggc gggctgtcgt ctgccatttg
     1201 cttgacgggt accggtaccg accgccatta tagtcgcgaa cccgatgacg gatctcgatc
     1261 aggaggggat ctccggtcgg ctgcgggccc gcttcgtcac ctatcaggag gcgccgcccc
     1321 gacgccgata cgccctgctc ggtctcacgc tgctcgcgct cgtcgcgatc ggctacggga
     1381 tctacacctg gtactactgg cgccatcaca tctccaccga cgacgcctac atcaccgccc
     1441 acatcgcgcc gatgagcgcg cgggtgcccg gcatcgtggt cgaggtggcg gtcaacgaca
     1501 accaggacgt gaagcccgac caggtgctgc tgcgcctcga cccgcgcgac tacgaggtcg
     1561 cggtggccca ggcgcgcgcg gcggtggtct cggccaaggg cgatctgcag aacgcggtgg
     1621 tcagcgtgcc gctcatcgac gactccacga gaagtctcgt ccagcaggcc gacgccgcgc
     1681 tcggcgccac ccagcacggc accgagatgg cccgccagga cctcgagcag cgccacgccg
     1741 agatccagtc gcgcctcgcc gcggtggccg ccgcggaggc ggcggtgcgc tcggcccagg
     1801 ccgactacga ccgcgccaag ctggaccgcg atcggaccgt ggcactcgtg aagagcttgc
     1861 tggtggccca gcaggagatg gaccacgcgg aggccaccta ccgcaccacg gaggccgcac
     1921 tggacgcggc ccgcaagcgc ctcgaccagg cgcgcagcga cgtgttccag gctcaggcct
     1981 cccagcgctc gcagtcggcc gcggtcgccc agtcggtgcg acgggtggat gagagccgcg
     2041 cgtcgctcga gaacgcgcgc agccagcgcc agcaggtggg ggtgcgccag gcccaggtgg
     2101 aggccgcgca ggggcgcctg gctcaggcga tcgccaacct gcagcaggcg gagctgaacc
     2161 tggtctactg cacgatccgg gcgccgatgg cgggccgggt gagccgcaag tcggtcgagc
     2221 tggggcaggt ggtgaccgcg ggccaggcgc tgctcgcggt ggtggacctc gacgacgtgt
     2281 gggtgctggc caactacaag gagaccgagc tgaccgacgt gcggcccggg cagtcggcga
     2341 cggtcaccgt cgacacctat ccgggcaaga agttccgcgc gcgggtcgac tcgatccagg
     2401 ccggctcggg cgcggtgttc tcgctgctgc cgcccgagaa cgccaccggc aacttcgtga
     2461 aggtggtgca gcgcgtgccg gtgaagctgc tcttcgagcc cggcgagaac gcccagcacc
     2521 tgctcgtgcc cggcatgtcc gtagtcccga tcatctccat ccgctgactc tccctctccc
     2581 ctccggggag agggcagggt gaggggagca tggggtgagg agcgtatgaa gatcgacgtg
     2641 cttctgattc ccgtcggcgc gcgctatccc gacatgcgcg ccgccgcgct cgcggccgag
     2701 gagtccggct tcgacggggt gtggacctgg gatcacctgc gcgatcccga cgaggcggcc
     2761 gagtccggcg tgccggaggc gtggacggtg ctgaccgcac tcgccgaggc cacgcggcgc
     2821 atcaccctcg gccccctcgt gctgaacacc gggctgcgcc atcccggcct gctcgccaac
     2881 atggcggcca cgctccagca ggtctccggc ggccggctgc tgctcggcat cggcgcgggc
     2941 gccacgccct cgat
//
LOCUS       DATMCV010000419         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_424772, whole genome shotgun sequence.
ACCESSION   DATMCV010000419 DATMCV010000000
VERSION     DATMCV010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424772"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..933
                     /locus_tag="VNU03_09740"
     CDS             136..933
                     /locus_tag="VNU03_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74787.1"
                     /translation="MTPAPTGRIIPLVPGYPLSADSIGAEPGEQFTWVNGDPARGEIQ
                     LEFDRVAGLPEVSSKSGVYTARFAAPGIYPYTVTGLSRSGVQLVPRRGEVVVREPTPA
                     AAPGAPRPEPAAPARPPSPPPRRDTPPAGADISRLKGSAEVYLAYHYQSEHGIVLKVE
                     RGAAAPTSLRPGSQVVLAVTYTVIAPPEVPPLVVKETRTIRFGDQDLRRLEKTVTVAP
                     GTYSSEQRLTVPSDAAEGPYTVTTTIELPSAARLQGQVSSAFSVRLP"
     gene            939..>2274
                     /locus_tag="VNU03_09745"
     CDS             939..>2274
                     /locus_tag="VNU03_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWP74788.1"
                     /translation="MPLPFGTPEEFAACRRLLQDVGYTEDALLAHHHIDTLSRLSSDR
                     GRAESVDDRLDLLTQLFVDGHYAARGQIESLLSPGALETLTALGLLAPSARNRELWAG
                     TVMLYPRAGLYFVSDRPRSVDGAPTTPMQDIVFSALTDDTERFLDLLPDEPCDTLLDL
                     GTGAGVAALIAARSARHVWAVDITERAARFAEFNQRLNGVANVTVLQGDLCAPVHDLR
                     FDRIVLHPPYVPALRQTLIYQDAGLDGQDITRRAVAQLVRHLAPGGRFYCLTVGVDCE
                     DAPFEQTLRLWLEPDDPEFDVAFIPRQAISVGFMARSVALKSGQGAPGAARLEDAFGR
                     LKIREFVYGAIVIQRHRASRPAFTVRRQRSPAGGRAAVAWLLRWETELAGRGSEWLLD
                     TRPRAAPTVEFLVTHRMVAGELSPTAYRMQTDHPFSTEARSAAWMGALIARCD"
BASE COUNT          293 a          844 c          784 g          353 t
ORIGIN      
        1 ctccgttgct gccggaagag aacggctctg tgctaggtta ggcgcggctt cgccatgcga
       61 acgtccagga cggggcaatc cgtgagccga ctactcggcg ctctgatcgt tgcgctatcg
      121 ggcgtggcgg cgtgcgtgac cccggccccg accggccgga tcatcccgct cgttccgggt
      181 tacccgctct ccgccgactc gatcggcgcg gagcccggcg agcagttcac gtgggtgaac
      241 ggcgatcccg cgcgaggtga gatccagctc gagttcgacc gcgtggcggg cctgccggag
      301 gtgtcgtcga agtccggtgt ctacacggcc cgcttcgccg cgccaggcat ctacccgtat
      361 acggtgaccg ggctcagccg cagcggggtc cagctcgttc cgaggcgggg cgaggtggtg
      421 gtgcgcgagc cgaccccggc cgcggccccc ggggcgcccc gacccgagcc ggcggccccc
      481 gcccgacccc cgtctccgcc tcctcgacgc gacacgccac cggccggcgc ggacatctca
      541 cggttgaagg gcagcgccga ggtctacctc gcgtaccact accagtcgga gcacggcatc
      601 gtcctcaagg tggagcgggg cgcggccgcg cccacctcgc tccgtcccgg atcccaggtc
      661 gtgctcgcgg tcacgtacac ggtcatcgcg ccgcccgagg tgccgccgct ggtggtcaag
      721 gagacccgga ccatccggtt cggcgatcag gatctgcggc gcctcgagaa gacggtgacg
      781 gtggctccgg gcacctactc cagcgagcag cggctcaccg ttccgtccga cgcggcggag
      841 ggaccgtaca ccgtgacgac gaccatcgag ctcccgtcgg ccgcgcggct ccagggtcag
      901 gtgtcgtccg cattctcggt caggctcccc tgaccggagt gcccctcccc ttcgggacgc
      961 ccgaggagtt cgccgcgtgc cggcggctcc tgcaggacgt cggctacacc gaggatgcgc
     1021 tcctcgccca ccatcacatt gacaccctgt cgcgcctgtc ttcggaccga ggccgggcgg
     1081 aatcggtcga cgaccgcctg gacctcctga cccagctctt cgtcgatggc cactacgccg
     1141 cgcgcggtca gatcgagtcg ctgctctcgc cgggtgcgct ggagacgctg accgcgctgg
     1201 ggttgctggc cccgagcgct cgcaatcgcg agctgtgggc cggcaccgtg atgctgtacc
     1261 cgcgcgcggg cctctatttc gtgtccgacc ggccccggag cgtcgacggc gcgcccacga
     1321 cgcccatgca ggacatcgtg ttctccgcgc tgaccgacga cactgagcgc ttcctcgacc
     1381 tgctgccgga cgagccgtgc gatacgctcc tcgatctcgg caccggggcc ggcgtggccg
     1441 cgctgatcgc ggcccgttcg gcccgtcacg tgtgggccgt cgatatcacg gagcgcgccg
     1501 cgcgctttgc ggagttcaac cagcggctca acggcgtggc caacgtgacg gtgctgcagg
     1561 gcgacctgtg cgcgccggtc cacgatcttc gcttcgaccg gatcgtcctc cacccgccgt
     1621 acgtgcccgc gctccgccag accctcatct accaggatgc cggcctggac ggccaggaca
     1681 tcacccgccg ggcggtggcc cagctcgtcc gtcatctcgc tcccggcggc cggttctact
     1741 gcctcaccgt cggcgtcgac tgcgaggacg ccccgttcga gcagacgctc aggctgtggc
     1801 tcgagcccga cgatcccgaa ttcgatgtcg cgttcattcc ccggcaggcc atcagcgtcg
     1861 gcttcatggc gcgaagcgtg gcgctcaagt cgggccaggg ggcgccgggc gcggctcggc
     1921 tggaggacgc gttcggccgg ctgaagatcc gcgagttcgt ctacggcgcg atcgtgatcc
     1981 agcgccaccg cgcctcacgt ccggcgttca ccgtccggcg tcagcgatcg ccggcgggtg
     2041 ggcgcgctgc ggtcgcctgg ctcctgcgct gggagaccga gctggccggc cggggctcgg
     2101 agtggctgct cgacacccgg ccgcgggccg cgccgacggt cgagttcctc gtcacccatc
     2161 ggatggtggc gggcgagctc tcgccaaccg cctaccggat gcaaaccgat catccgttca
     2221 gcacggaagc gcggtcggcg gcgtggatgg gcgccctcat cgcgcgatgc gacg
//
LOCUS       DATMCV010000420         7086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_685495, whole genome shotgun sequence.
ACCESSION   DATMCV010000420 DATMCV010000000
VERSION     DATMCV010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685495"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VNU03_09750"
     CDS             <1..1446
                     /locus_tag="VNU03_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWP74789.1"
                     /translation="MSAAAKLGWGTGWVVVLALVLTGCFAKVKDLPALAEKPAFAPIP
                     EAAPDLLVGLAISGGGSRAATFGAGVLEALARVRVKDARGERSVLEKVQYISSVSGGS
                     LATGYFAARKPPRSQPVLGSSGLSPAYEQFFKDYKAAMQEDFELPAAWRQVLLFRAFN
                     PTKLAYSLAEVWDARFFHGMTFGQLYERERAGDSPRIILNGTSYNSGRRFALTTLPAA
                     DFDYDFVGGLLQDLMRRSDRVTPEGKAIIQRNLEGARDQFLPMTFEQIGADHRRLPLS
                     LGVVTSASFPPVVGPVTYGIAGRPPYQHVGDGGLFDNLGTESLTTLFLKKIPKGSPKR
                     GLIIVIDSAFPFDANAAVLDTNPRGYQVFKKDPSRIVGIMEERANAYQLMLWDALRTE
                     GVALPDFANLRVEVLQHTEPDWGTYQDLPEECRGEFDANVTAKAVKDTVSEIPTLFRI
                     SKCHGALMIKAAEKVVEKHRARIVEFLERSR"
     gene            1475..3976
                     /locus_tag="VNU03_09755"
     CDS             1475..3976
                     /locus_tag="VNU03_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74790.1"
                     /translation="MSRRRLVTLGTIVIACAAVFVLGRPAPGEQAGPVLDEARQAGLP
                     AKHFLDHVADEDYFHDMDGAIALTPAEIKGRNTWLVWTGGNDRLWDVLSTASFGNLDF
                     LKTVSSHPGLKFSRDNRWSYLGLVNEPCFEKATGPDPGRFGLWLDKRRADCPADPFEN
                     EAKYPGVPVGARGKDLGDGRKLPVGSSYGYASGIVGLRLFPNPAFDAEAARKWDATRY
                     YEDPAYYLSKDLVRPYRVAMSCAFCHVGPNPLKPPADPENPRWANLSSNVGAQYFWLD
                     RIFGWDANPSSFIVQLFRTSRPGTLDTSLVSTDNINNPRTMNALYHLGPRLGRARQVG
                     KETLGPGSRDNKQFNDFVGSGPLTQFFEPPATVWTPRVLKDGADSVGALGALNRVYLN
                     IGLFSEEWLLHFNPLIGGKRTTPIQIKLARENSSYWGATEGQTVDMALFFLKSTGPHL
                     LKDAPGGAAYLTRDQEKLTRGREVFAERCARCHSTKTPEVPATLGLRACEGPGYLECF
                     KTYWRWTKTDDYKRRMREIASRGDFLDDNLLASEVRVPVTLLETNACSPLATNAIRDN
                     IWDNFSSETYKNLPSVGEITVHHPITGAPYQYRMPAGGRGYTRPASLVSVWSTAPFLL
                     NNSVGHFEGSPSVEARMRSFQDSIEKLLWPELREYDKLLGTKVPGLIDRTTAPSYLRV
                     PAGYLPDFLQPLVGRLHRIFPTIFGDGGLEIGPIPTDTPVGLLANLNLVSEDSGVVDR
                     VKYQARVFALLVKAKHDLGRLSERASDTEARAIFANLVPDLLKLSKCPDFVVNRGHYF
                     GTGFFRDPYKESGEVDEPGLSDADKRALIEFVKTF"
     gene            3977..5962
                     /locus_tag="VNU03_09760"
     CDS             3977..5962
                     /locus_tag="VNU03_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWP74791.1"
                     /translation="MKLDRRTFLKAAAALGAAVGRPPGAAVPPAAAQPSTPSGGAASA
                     DAECDYVVVGSGAGGGTVAARLAEAGYRVVLLEAGGDPMQLQGGNAAYPDAARLPDDY
                     QVPCFHALSTENEAMKWDFFVRHYADETQQRRDSKYVTEERGVLYPRAGTLGGCTAHN
                     AMIMVYPHNEDWDRIAELTRDESWRADRMRAYFERLEDCRHRPLARFFGRLGINPSRH
                     GWGGWLSTEKAIPLAALMGDPELIQVIQESALRAFAETGSPIEALRGLTDSRADPNDW
                     RMVSDRAVGIRYTPLTTGHHQRVGTRERVRDVERRYPERLQVRLHALATRVLFAPDNT
                     AVGVEYLEGPRLYRAHSGVSARPGVLRQVRASREVILAGGAFNTPQLLMLSGVGPADG
                     LTRHGIPVRVDLPVGKNLQDRYEVGVVNRMSRDWNVLEGARFDKDDAQYAQWAARRDG
                     VYTTNGAVLAVVKASGFEPSGLPDLFCFGLLGRFAGYRPRYSVELRDHHNYLTWAVLK
                     AHTVNQGGEVTLASPDPRDRPDVNFRYFEEGTDAGRRDLDAVVDGVRFVRKMTADLKR
                     RGRIVEEELPGDRCVSDEDLRTFVRDNAWGHHASCTCPIGPVLDSELRVLGTRRLRVV
                     DASVFPRIPGFFIASAVYMVAEKAADALVATAKRESA"
     gene            6094..6927
                     /locus_tag="VNU03_09765"
     CDS             6094..6927
                     /locus_tag="VNU03_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HWP74792.1"
                     /translation="MAILTISQEMGSGGAEIGMAVATRLGYTYVDQEELLGRAKRYGL
                     AEDRLARLAEDRPSWVERFDAETRRCILALQVVLYEFAQDDNVVLMGGGGQWLLRGLP
                     HVLRTRIVAPFPVRVTRLTATLSGQGKERVTPKTVAQFIRRDDIQKAARMRYLFDAEV
                     KDPSLYDLLINTAVLSREAAVGLLAEAARRSELATTDAARDLVVDRVVASQVEVALAG
                     HPEMRRHRIDVECVSGEVTLELPGGADPNVALAVARGAAGTQNVKLRIAEIPVVTPFP
                     G"
BASE COUNT         1075 a         2468 c         2487 g         1056 t
ORIGIN      
        1 atgagtgcgg ccgcgaagct cgggtggggg acgggttggg tcgtggtgct ggcgttggtg
       61 ctcaccggct gctttgccaa ggtgaaggat cttcccgcgc tcgccgagaa gccggcgttc
      121 gcgcccatcc cggaggccgc ccccgacctg ctcgtggggc tggcgatctc gggcggagga
      181 agccgggcgg ccaccttcgg ggccggcgtc ctggaagcgc tcgcccgcgt ccgggtgaag
      241 gatgcaaggg gggagcgcag cgtgctggag aaggtgcagt acatctcgag cgtgtccggg
      301 ggcagcctcg ccaccggata cttcgcggcg cgaaagccgc cgaggtccca gccggttctg
      361 ggaagctcgg ggttgtcgcc cgcctacgag cagttcttca aggactacaa ggcggcgatg
      421 caggaggact tcgagctgcc ggcggcctgg cgccaggtgc ttctgttccg cgcgttcaat
      481 cccacgaagc tcgcctactc cctcgccgaa gtgtgggacg cgcggttctt ccacgggatg
      541 accttcggcc agctctacga gcgcgagcgg gcgggggata gcccgcggat catcctgaac
      601 gggacctcgt acaactcggg ccgccggttc gccctcacca cccttcccgc cgccgacttc
      661 gactacgact tcgtgggcgg gctgctccag gacctcatgc ggcgctccga ccgggtgacc
      721 ccggagggga aagcgatcat ccagcgcaac ctggagggcg cgcgggacca gttcctgccg
      781 atgacgttcg agcagatcgg cgccgatcac cggagacttc ccctctcgct aggagttgtg
      841 acctccgcct cgttcccgcc cgtggtcggg ccggtcacct acgggatcgc gggtcggccg
      901 ccgtaccagc acgtgggcga cggcgggctc ttcgacaacc tgggtaccga gtccctgacc
      961 acgctgttcc tcaagaagat cccgaagggc tccccgaagc ggggcttgat catcgtgatc
     1021 gactcggcct tcccgttcga cgcgaacgcc gcggtcctcg atacgaatcc gagaggctac
     1081 caggtgttca agaaggatcc gtcgcggatc gtcggcatca tggaggagcg ggccaacgcg
     1141 tatcaactca tgctgtggga cgcgctccgg accgaggggg tcgcgctgcc cgacttcgcc
     1201 aatctgcggg tggaggtcct ccagcacacc gagcccgact ggggcaccta ccaggacctg
     1261 cccgaggagt gccggggcga gttcgacgcc aacgtgacgg ccaaggcggt caaggacacg
     1321 gtgagcgaga tcccgaccct ctttcgcatc tcgaagtgtc acggggcgct catgatcaag
     1381 gcggcggaga aggtggtgga gaagcatcgc gcgcggatcg tggagttcct cgagcggtcg
     1441 cggtagccga cgaccacgaa agcgagggtt cgccatgagt cgacgacggc ttgtcaccct
     1501 gggcaccatc gtgatcgcct gcgccgcggt gttcgtcctg ggccgcccgg cgcccggcga
     1561 gcaggcgggg ccggtgctcg acgaggcgcg ccaggcgggc cttcccgcga agcacttcct
     1621 cgaccacgtg gccgacgagg actacttcca cgacatggac ggggcgatcg cgctcacccc
     1681 cgccgagatc aagggtcgca acacctggct cgtgtggacc ggcggcaacg atcggctgtg
     1741 ggacgtgctg tcgaccgcga gcttcggcaa cctcgacttc ctcaagacgg tgtcgtcgca
     1801 tccgggtctc aagttcagcc gcgacaaccg ctggagctac ctcggcctcg tgaacgagcc
     1861 ctgcttcgag aaggccaccg ggccggaccc ggggcgcttc ggcctctggc tcgacaagcg
     1921 tcgcgcggac tgcccggccg atcccttcga gaacgaggcc aagtacccgg gggtgccggt
     1981 gggggcgcgg ggcaaggacc tgggggacgg acggaagctg ccggtcggct cgtcctacgg
     2041 ctacgcgagc gggatcgtgg gcctgcggct cttccccaac cccgccttcg acgcggaggc
     2101 cgcgcggaag tgggacgcga cgcgctacta cgaggacccg gcctactatc tgtcgaagga
     2161 cctcgtgagg ccctaccggg tcgcgatgtc ctgcgcgttc tgccacgtcg ggccgaaccc
     2221 gctcaagccg ccggccgacc cggagaaccc ccgctgggcg aacctcagct ccaacgtggg
     2281 ggcccagtac ttctggctcg accgcatctt cggctgggac gcgaacccgt ccagcttcat
     2341 cgtccagctc ttccgcacct cgcggccggg gacgctcgat acgtcgctcg tctccaccga
     2401 caacatcaac aacccgcgca ccatgaacgc gctctaccac ctcggccccc gcctcgggcg
     2461 cgcgcgccag gtgggcaagg agacgctcgg gcccggcagc cgcgacaaca agcagttcaa
     2521 cgacttcgtc gggagcgggc cgctcaccca gttcttcgag ccgcccgcga cggtgtggac
     2581 gccgcgggtg ctgaaggacg gcgcggattc ggtcggtgcc ctcggcgcgc tcaaccgggt
     2641 gtacctgaac atcggtctgt tcagcgagga gtggctgctg cacttcaacc cgctgatcgg
     2701 aggcaagcgg accaccccga tccagatcaa gcttgcgcgc gagaactcga gctactgggg
     2761 ggccaccgaa ggccagaccg tcgacatggc gctcttcttc ctcaagagca ccggcccgca
     2821 cctcctcaag gacgcacccg ggggggccgc gtacctgacg cgcgatcagg agaagctgac
     2881 ccggggccgc gaggtgttcg ccgagcgctg cgcgcggtgc cactcgacca agacccccga
     2941 ggtgcccgcc acgctcggcc tgcgcgcgtg cgagggcccc ggctatctcg agtgcttcaa
     3001 gacctactgg cgctggacca agaccgacga ctacaagcgc cggatgcgcg agatcgcgag
     3061 ccggggcgac ttcctggacg acaacttgct cgccagcgag gtccgggttc cggtcaccct
     3121 gctcgagacc aacgcctgca gcccgctggc caccaacgcg atccgggaca acatctggga
     3181 caatttctcg tccgagacct acaagaacct gccgtcggtg ggcgagatca cggtccacca
     3241 tccgatcacc ggcgcgccct accagtaccg gatgcccgcc gggggccggg gctacacccg
     3301 cccggcctcg ctcgtgagcg tgtggtcgac cgctccgttc ctgctcaaca acagcgtggg
     3361 ccacttcgaa ggcagcccct cggtcgaggc gcggatgcgc tcgttccagg actcgatcga
     3421 gaagctgctc tggccggagc tgcgcgagta cgacaagctg ctcggcacca aggtgcccgg
     3481 cctcatcgac cggaccaccg cacccagcta cctgcgcgtc cccgccgggt acctccccga
     3541 cttcctccag ccgctcgtcg ggcggctgca ccggatcttt ccgacgatct tcggcgacgg
     3601 ggggctcgag atcggcccca ttcccaccga caccccggtg ggactgctgg ccaacctgaa
     3661 cctggtctcg gaggattcgg gcgtggtcga ccgcgtcaag taccaggccc gggtgttcgc
     3721 gctgctcgtg aaggcgaagc acgacctggg ccggctttcg gagcgcgcca gcgacaccga
     3781 ggcgcgggcg atcttcgcga acctcgtgcc ggatctgctg aagctgagca agtgcccgga
     3841 cttcgtggtc aatcgcgggc actacttcgg caccgggttc ttccgggacc cctacaagga
     3901 gagcggcgag gtcgacgagc ccggcttgag cgacgccgac aagcgcgcgc tgatcgagtt
     3961 cgtcaagacg ttctagatga agctcgatcg acgcaccttc ctgaaggcgg ccgccgcgct
     4021 cggggccgcg gtggggagac cgccgggagc ggcggtgccg cccgcggccg cccagccgtc
     4081 gacgccgagc ggcggcgcgg cctcggcgga cgccgagtgc gactacgtgg tcgtcggctc
     4141 cggcgcgggc ggcggcacgg tggccgcgcg tctggccgag gccggctacc gggtcgtgct
     4201 gctggaggcg ggcggcgacc cgatgcagct gcagggcggc aacgccgcgt atccggacgc
     4261 ggcccgcctt ccggacgact accaggtgcc ctgcttccac gccctctcga ccgagaacga
     4321 ggcgatgaag tgggacttct tcgtccggca ctacgccgac gagacccagc agcggcgcga
     4381 ctccaagtac gtgacggagg agcggggcgt gctctatccc cgggcgggga cgctcggcgg
     4441 gtgcaccgcc cacaacgcca tgatcatggt ctacccccac aacgaggact gggatcggat
     4501 cgccgagctg acccgggacg agtcatggcg cgcggatcga atgcgcgcgt acttcgagcg
     4561 gctcgaggac tgccgccacc ggcccctcgc ccgcttcttc ggccggctcg ggatcaaccc
     4621 gagccgccac ggctggggtg gctggctgtc caccgagaag gcgattcccc tcgcggccct
     4681 catgggcgat ccggagctga tccaggtgat ccaggagtcg gcgctccggg ccttcgcgga
     4741 gaccggctcc ccgatcgagg cgctccgcgg cctcaccgac agccgcgccg atccgaacga
     4801 ctggcggatg gtgagcgatc gggcggtcgg gatccgctac accccgctga ccaccgggca
     4861 ccaccagcgg gtggggacgc gagagcgcgt gcgcgacgtg gagcgccggt acccggagcg
     4921 gctgcaggtg aggctccacg ccctcgccac acgggtgctg ttcgcgcccg acaacaccgc
     4981 ggtgggcgtc gagtatctcg agggtccgcg cctgtaccgc gcgcactccg gggtgagcgc
     5041 ccgcccgggc gtgctgcggc aggtccgggc ctcccgggag gtcatcctgg ccggcggggc
     5101 gttcaatact ccgcagctcc tgatgctgtc cggcgtggga cccgcggatg gcctcacccg
     5161 gcatggcatc ccggtgcggg tggaccttcc ggtggggaag aacctgcagg accgctacga
     5221 ggtcggcgtg gtcaaccgga tgtcacggga ctggaacgtg ctcgagggcg cgcggttcga
     5281 caaggacgac gcccagtacg cccagtgggc ggcccgacgg gacggggtgt acacgacgaa
     5341 cggtgcggtg ctcgcggtgg tgaaagcctc cgggttcgag ccgtcgggct tgccggacct
     5401 gttctgcttc ggcctgctgg gccgctttgc gggctaccgg ccgcgatact cggtcgagct
     5461 gcgcgaccac cacaactacc tcacctgggc ggtgctgaag gcgcacaccg tcaaccaggg
     5521 cggcgaggtg acgctggcgt ccccggaccc gcgtgaccgg cccgacgtca acttccgcta
     5581 cttcgaggaa ggaaccgacg cgggtcgccg cgatctcgac gcggtcgtcg acggggtgag
     5641 gttcgtgagg aagatgaccg cggacctcaa gcggcgtggc cggatcgtgg aggaggagct
     5701 gccgggcgac cggtgcgtgt ccgacgagga cctccggacc ttcgtccggg acaacgcgtg
     5761 ggggcaccac gcctcgtgca cctgcccgat cggcccggtg ctcgactccg agctccgcgt
     5821 gctcggcacc cggcgcctgc gcgtggtcga cgcctcggtc tttccaagga tccccggctt
     5881 cttcatcgcg agcgcggtct acatggtcgc ggagaaggcg gcggacgcgc tggtcgcgac
     5941 cgcgaagcgc gagagcgcct agccgggcct cgcgggcgaa gcgtttacag gggcttgact
     6001 cggtcgcccg ccgagaataa gcttccgcca ccgttcgacc ggctcggccc ggctcgcccg
     6061 acccgagggc ccgcggggca ggaggatcac gccatggcga ttctgacgat ctcccaggaa
     6121 atgggcagcg gcggcgccga gatcggcatg gcggtggcaa cccgcctcgg ctacacctac
     6181 gtcgatcagg aggagctgct gggccgggcg aagcgctacg gcctggcgga ggatcgtctc
     6241 gcccgtctcg ccgaggaccg gccatcctgg gtggagcgct tcgacgcgga gacccgccgg
     6301 tgcatcctcg cgctgcaggt ggtcctgtac gagttcgcgc aggatgacaa cgtcgtcctg
     6361 atgggtggcg ggggccagtg gctgctccgg ggactgcccc acgtgctccg gacccggatc
     6421 gtggcgccct ttccggtgcg cgtgacgcgg ctgaccgcga ccctgtccgg ccagggcaag
     6481 gagcgcgtca ctccgaagac ggtcgcccag ttcatccgcc gggacgacat ccagaaggcg
     6541 gcgcggatgc gctatctctt cgacgccgag gtcaaggacc cgagcctcta cgacctcctc
     6601 atcaacaccg cggtgctctc ccgcgaggcg gccgtcggtc ttctcgcgga ggcggcgcgg
     6661 cgatccgagc tcgccaccac ggacgcggcc cgggatctgg tagtcgatcg cgtggtcgcc
     6721 tcccaggtgg aggtcgcgct cgccggccac ccggagatgc ggcgccaccg gatcgacgtg
     6781 gagtgcgtga gcggcgaggt gacgctggag ctgcccggcg gggccgatcc caacgtggcg
     6841 ctcgcggtgg cccggggcgc ggcggggact cagaacgtca agctccggat cgcggagatc
     6901 ccggtggtga ccccgttccc ggggtgagcc ggcccggtct tccgcctctc ttcggctccg
     6961 gcccggccgt cccgcggacc tagggccggc ctcgcttcgc ggctcccatg acgtccccat
     7021 ttcgatgggt cctcaccgcc tttcgagacc tcggcctgaa cgtcttcggc ttcctcctga
     7081 tgttcg
//
LOCUS       DATMCV010000421         3319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_489785, whole genome shotgun sequence.
ACCESSION   DATMCV010000421 DATMCV010000000
VERSION     DATMCV010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489785"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VNU03_09770"
     CDS             complement(<1..801)
                     /locus_tag="VNU03_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74793.1"
                     /translation="MSVLRAIAARVAAHPVLAFWIFFAVFPFIVPNRTMATQVLIFGL
                     LAMGFNLLYGYTGLLSFGHAAYYGLGAYGCGIALAKLHVGSLWLGLGAGFFLAGVGGL
                     VIGFFCLRRRGIYFAMLTLAFAQLLYFIGFHLADVTGGDDGLRGIPQLALGLGPWRYS
                     IETTIAFYYFAYVLVGLAVLALRRILDSPFGAVLQAIRENSSRAVACGYDVDRVKHLS
                     FVFSACFAGLAGSLDALRLTVVPVESLYWTTSGQVVMMTLLGGAGTFFG"
     gene            complement(798..1676)
                     /locus_tag="VNU03_09775"
     CDS             complement(798..1676)
                     /locus_tag="VNU03_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74794.1"
                     /translation="MPEFSTLLAQVFTGLVLGMIYVLLAIGLSLIFGLMTVVNFAHGA
                     LFMLGAYFGVFVLTYTKSFWIALIVAPLMVGALGLLMERFLIRRLYGRSPDDPLLLTF
                     GLSLILVEGVKLLWGKIGLTLDPPRALAGAVNLGFMSFPAYRIFLIGVTVAVLIGLYF
                     FLGRTNIGLIIRAGSRDPLMVRALGIDLGRVWLVVFGIGTALAGLAGILAGPMRGAYA
                     EMGVTMVIESFVVIVVGGMGSLLGAVVAGLLIGQVVGLTTLFMPKAAEIMVFVVMAVV
                     LLVRPSGLFGEAGLAE"
     gene            complement(1681..2892)
                     /locus_tag="VNU03_09780"
     CDS             complement(1681..2892)
                     /locus_tag="VNU03_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74795.1"
                     /translation="MDRRSFLATTAAGAAALSFPAILRAQSKDPFRVGCPLPLTGPFA
                     ALAADMQRGAQLAEAELNARGGVMGRKVEVLFRDDQLKPAVGAQRTKELIENDKCQFI
                     VGGLAAHVQMAINEQTKKSKVLYISTSQSDEISAKPDTSPITFHEALNPTITCRVLGK
                     WAAENLGKKWWIIYADYAWGKQCNQVLQDALKKSGGTLLGATPYPLGSAEFSAHLPKI
                     QAAKPDVLMNVAPGADNIALHKQVISFGMKKDMKIAQPLLWLSYLKEGGPELYQDVYG
                     AVNWYWELQDSIPTAKKFVDASMKKFNLPPGDYGAYSYSGVLEVARGTELAKSTDAEA
                     VADALRKNPTYDHFKGKQWWRACDNKSFQDLWIVKGRDKVKGDWGLMDIVTKVGANEE
                     FDRTCSEKGFA"
     gene            2988..>3319
                     /locus_tag="VNU03_09785"
     CDS             2988..>3319
                     /locus_tag="VNU03_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWP74796.1"
                     /translation="MPHVLRHVGARLKRPDDPRLLTGRGRYLDDLTLPRMAHVAFVRS
                     PHAHAVIAGIECDAARLCPGVLAVLTGPEAARFCKPYRGVLLHYAGMKTGAMLPLATD
                     RARYVGEPV"
BASE COUNT          572 a         1121 c         1154 g          472 t
ORIGIN      
        1 cccgaagaag gttccggccc cgcccaggag cgtcatcatg accacctggc ccgaggtggt
       61 ccagtatagc gactcgaccg gcacgacggt gagccgcagc gcgtcgagcg atccggcgag
      121 cccggcgaag caggcggaga acacgaacga cagatgcttg acgcgatcga cgtcgtaccc
      181 gcaggccacc gcccggctgc tgttctcgcg gatggcttgc agcaccgccc cgaacggcga
      241 gtcgaggatg cgccgcagcg ccagcaccgc cagcccgacc agcacgtagg cgaagtagta
      301 gaaggcgatg gtggtctcga tcgagtagcg ccaggggcca aggcccagcg cgagctgggg
      361 gatgccgcgc agcccgtcgt cgccaccggt cacgtcggcc aggtggaagc cgatgaagta
      421 gaggagctgg gcgaaggcca gggtcagcat cgcgaagtag atgccgcgcc ggcgcaggca
      481 gaagaagccg atgacgaggc cgcccacgcc ggcgaggaag aaccccgcgc cgagcccgag
      541 ccagagcgag cccacgtgga gcttggcgag cgcgatgccg cagccgtagg cgccgaggcc
      601 gtagtaggcg gcgtggccga aggagagcag gccggtgtag ccgtagagca ggttgaagcc
      661 catggccagc aggccgaaga tcagcacctg ggtcgccatg gtgcgattgg gcacgatgaa
      721 ggggaagacc gcgaagaaga tccagaacgc gagcaccggg tgcgcggcca cgcgcgcggc
      781 gatcgcgcgg agcacgctca ttcggccagc cccgcctcgc cgaagagacc gctcggccgc
      841 accagcagca ccaccgccat gaccacgaag accatgatct ccgccgcctt gggcatgaag
      901 agggtggtga ggcccaccac ctgcccgatg agcaggcccg ccaccaccgc gccgagcagg
      961 ctgcccatcc cacccaccac gatgaccacg aacgactcga tcaccatggt cacgcccatc
     1021 tccgcgtagg cgccgcgcat cggcccggcc aggatgccgg ccagcccggc gagcgcggtg
     1081 ccgatgccga agacgaccag ccagacgcgc ccgaggtcga tgccgagcgc ccggaccatc
     1141 agcgggtcgc gcgagcccgc gcggatgatg aggccgatgt tggtgcgtcc gaggaagaag
     1201 tagaggccga tcagcaccgc caccgtgacc ccgatgagga agatgcgata ggccgggaag
     1261 gacatgaagc ccagattcac cgcgccggcc agggcccgcg gcggatcgag ggtgagcccg
     1321 atcttgcccc acaggagctt gaccccctcg accaggatca gcgagaggcc gaaggtgagc
     1381 agcagcggat cgtcgggcga gcggccgtag agccgccgga tgaggaaccg ctccatcagg
     1441 agccccagcg ccccgaccat gagcggggcc acgatcaggg cgatccagaa gctcttggtg
     1501 taggtcagca cgaagacgcc gaagtacgcc cccagcatga acagcgcgcc gtgcgcgaag
     1561 ttcaccaccg tcatcagccc gaagatcagg gacaggccga tggcgagcag gacgtagatc
     1621 atcccgagca cgaggccggt gaagacctgt gccaggagcg tggagaactc gggcaccggg
     1681 ctaggcgaac cccttctcgg agcaggtgcg gtcgaactcc tcgttcgccc ccaccttggt
     1741 gacgatgtcc atcaggcccc agtctccctt gaccttgtcg cggcccttga cgatccagag
     1801 gtcctggaac gacttgttgt cgcaggcccg ccaccactgt ttgcccttga agtggtcgta
     1861 ggtcgggttc ttgcggagcg cgtcggcgac cgcttccgcg tcggtggact tggccagctc
     1921 cgtgccgcgg gccacctcga gcacgccgct gtacgagtag gccccgtagt cgccgggcgg
     1981 gaggttgaac ttcttcatcg acgcgtcgac gaacttcttg gcggtgggga tggagtcctg
     2041 cagctcccag taccagttga ccgcgccgta gacgtcctgg tagagctccg gcccgccctc
     2101 cttcaggtag gagagccaga ggagcggctg ggcgatcttc atgtccttct tcatcccgaa
     2161 gctgatcacc tgcttgtgga gcgcaatgtt gtccgcgccg ggcgccacgt tcatcaggac
     2221 gtccggcttc gcggcctgga tcttgggcag gtgcgcggag aattcggccg acccgagcgg
     2281 gtacggagtg gcgcccagca gggtgccgcc cgacttcttg agcgcgtcct gcagcacctg
     2341 gttgcactgc ttgccccacg cgtagtccgc gtagatgatc caccacttct tgccgaggtt
     2401 ctcggccgcc cacttgccca gcacccggca ggtgatggtg gggttcagcg cctcgtggaa
     2461 ggtgatcggg ctcgtgtcgg gcttggcgct gatctcgtcg gactggctcg tggagatgta
     2521 gagcaccttg gacttcttgg tctgctcgtt gatggccatc tgcacgtgcg cggcgaggcc
     2581 gcccacgatg aactggcact tgtcgttctc gatcagctcc ttggtgcgct gcgcgcccac
     2641 cgcgggcttg agctggtcgt cacgaaagag cacctcgacc ttgcggccca tcacgccgcc
     2701 cctggcgttc agctccgcct ccgcgagctg ggcgccgcgc tgcatgtcgg ccgcgagggc
     2761 ggcgaacggc ccggtcagcg gcagcgggca gccgacccgg aacggatcct tggactgggc
     2821 gcgcaggatg gccgggaagg acagggccgc ggcgccggcc gcggtggttg ccaggaatga
     2881 gcgacgatcc atggagttgc ctcctctgcg ttgagtgagc gcgacgtctg cggcgacggg
     2941 tggcgggtat cataggaggc cctccggagc gtgtcaatcg tgttaccatg ccgcacgttt
     3001 tgcgtcacgt cggagcccgc ctcaagcgcc cggacgaccc ccgcctcctc accgggcgcg
     3061 gccgctacct cgacgacctc acgctgccgc ggatggccca cgtggccttc gtccgcagcc
     3121 cgcacgccca cgcggtcatc gccgggatcg agtgcgacgc ggcgcgcctc tgcccggggg
     3181 tcctggcggt cctgaccggg ccggaggcgg cgcggttctg caagccctac cgcggcgtgc
     3241 tgctccacta cgcgggcatg aagacgggcg cgatgctccc gctcgccacc gaccgcgcgc
     3301 gctacgtggg cgagccggt
//
LOCUS       DATMCV010000422         7147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_222268, whole genome shotgun sequence.
ACCESSION   DATMCV010000422 DATMCV010000000
VERSION     DATMCV010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222268"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2143)
                     /locus_tag="VNU03_09790"
     CDS             complement(<1..2143)
                     /locus_tag="VNU03_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP74797.1"
                     /translation="MGRLTRHFAIPFLVLAVPLGILAGLGFISAERQLARTREGAIGR
                     TVDAVIALVGDYQDALKRETLLLARDPAIIEGTAKGDWATLARGASPRVLAVTQDGLA
                     DFIAIRDGRGASLVQVPAGPPPSLPGMPASSEPITALRLAGGRPYFLVVAPISGATER
                     APFGATGTVVAGRRLDGLAASLARLPARPAVLFLAGDRALATSRADLPATGWTPAVGS
                     GIATVAGEPWALRRLDFPAEASPDGALWVALPVREFAQAERRLLFEFLALLAGGLVVL
                     AGVVLAFLPSAGRARPVAGPRTENPRIALERRNRELEALNAIATTIGRGANLETTARE
                     TLEVVRSLAGMDVGSVYQLDASGRELIMVAGSGFDPRHVETSRVRPVDGSRIGDAVRT
                     NQILVTHLDAVPPSEEGLRQMAAERAHRTQLAMPIPVEEQTWGVMALVSRETREFSDE
                     EMKILSSVAQQVGLAVERARLRDMAAARLSRLEAQRLIERHISEQLATEQLLVVIARA
                     AQRLIGGGYAALYMLEGDTLHPRAWSDIGDWVRDLRFTTDRGVAGAAMHTGRGVMVND
                     YPDSQHAMAEFVPFTSRLLAAPLMAGGRALGVMTAGRGPGAAPFTEEDFSTLTDFATQ
                     AAVALEHARLFDEATRNAAQYQALLEVSTVVSSTLEVDRVLDLVVDRCQALLGVAALG
                     ISRLDPSTGLLHYEGGRGLSAEFLSSLRIRLG"
     gene            complement(2195..2908)
                     /locus_tag="VNU03_09795"
     CDS             complement(2195..2908)
                     /locus_tag="VNU03_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74798.1"
                     /translation="MSTLLRVDGIEVGYGDLTAVRDVSLEVREGEAVALIGANGAGKT
                     TTLRAVSGLLPLRRGRIEFEGQRLDGLGPAEIVARGIAHVPEGRQLFPTMTVRENLEL
                     GARTRAARGSRAETLERVFELFPRLRERGAQLAGTLSGGEQQMCAIGRGLMARPRLLM
                     LDEPSLGLAPVLVKAIFEDLGRINGQGLTVLLVEQNVLRSLHLAHRGYVLENGRMTLS
                     GPRDDLLASRHIKQAYLGL"
     gene            complement(2886..3629)
                     /locus_tag="VNU03_09800"
     CDS             complement(2886..3629)
                     /locus_tag="VNU03_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74799.1"
                     /translation="MSTPPLLEVRGIGKRFGGLQAVRNLSFDMARGEVLGLIGPNGAG
                     KTTVFHLLSGFLAPDGGDVRFDGRSLAGLKPHAICRLGLARTFQIVRPFPHLSVWENV
                     RVGALARRPQMAEARRRAEAVIDQVGLGAKAGQPAGGLTLAERKRLELARALATEPTL
                     LLLDEVMAGLNPTEIEAIVALIQGIHASGISILLIEHNMRAVMSLSHRIVVLSFGEKI
                     VEGPPAAIANHPRVIEAYLGEEYVHAPAR"
     gene            complement(3626..4573)
                     /locus_tag="VNU03_09805"
     CDS             complement(3626..4573)
                     /locus_tag="VNU03_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74800.1"
                     /translation="MKWVVAAAIALLLALPLWLNSYAVTVFIFIFFYAFLGQAWNIVG
                     GYAGQLSAGHAAFVGIGAYTSALLSAQAGLTPWIGMLVGGVLAAVLGTVVGYLGFRFG
                     LRGFYFVLLTVAFAEVCRIVALNTDAVGGALGFYITFTGNPRQFQFQDNRVYYYVALG
                     LMLVATGLVAAIERRRFGAYLIAIREDEQACEALGVNTFRYKMLAMVVSSFLTGVGGT
                     FYAFYLFSLQPNSVFGIPLSVEIVIRPIVGGAGTVLGPILGSFILSPLAEASRIYFGQ
                     GSWSGAHLIAYGLLLIAVVLFLPQGAYPALRRLWVKAAR"
     gene            complement(4570..5442)
                     /locus_tag="VNU03_09810"
     CDS             complement(4570..5442)
                     /locus_tag="VNU03_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74801.1"
                     /translation="MDPALIAQGLLSGLLFGGVYSLMAVGLTLIFGVMRVVNFAHGDM
                     MMWGMYLAWLLATRAGIDPYIGFVACAAALFVLGVVIQRVLVDRVVDAPHEMQILLML
                     GVALVLENSALVAFGPDPQRVRSPLRAATIWLGPVFVDVARLVTFGLAMLLSLALYLF
                     LTRTDLGRTMRAAADNAYGARVIGADVRRVYAVAFGIGAACVGAAGALVSPIVPFQPA
                     GGLGLSVASFNIVIIGGMGSLPGAFLGGLIVSVAESLGAVFLSPSMKELVSFSLLIVI
                     LLFRPAGLFGRPAS"
     gene            complement(5449..6744)
                     /locus_tag="VNU03_09815"
     CDS             complement(5449..6744)
                     /locus_tag="VNU03_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74802.1"
                     /translation="MSDRRGPSRVHRRTFLKAGLKAAGTAAGAAALGFPAVLRAQVPT
                     FKVGAIHPVTGPLAEPGQACRLGAQMAADAINAAGGIKSLNGMKIDLIVGDTQTKPDI
                     GRTEAERVINQGAQMLMGSFDSGSTQAIVPVAQQRRVPFLVDIAAADPITANVAKAVK
                     EGQQKVQYVYRNFPTGSSFGRKAVQYFTEIFQEAKVSPKRVVLMYCNDLFGQNNAKGF
                     QAAHAAAKPGWEIVDVIPWPEPPQDLSTEVSRAKAAKPDIIAPITRPASAQLLLPEIR
                     KQRLEIMGIVGPGSPGLYEAGQIALLKEDLEYVLNSTPWANFKNARTQKVAEEYLKRS
                     QGKTFDTNSGYSYDGVSLIADVLERAKSTDPDAIVEAIHKTNWTAGLMQYAGPVHFNE
                     IGDNPNAVTTMLQILGGKPVAVWPKEAAIQKFVFPRPKA"
     gene            complement(6770..>7147)
                     /locus_tag="VNU03_09820"
     CDS             complement(6770..>7147)
                     /locus_tag="VNU03_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP74803.1"
                     /translation="LDASVAYAKERRAFDQPIAQFQGLQFMLADMAIAVHASRLVVHH
                     AAGLIDHGITGTALEASVAKCLASDTAMKVTTDAVQIFGGYGYTREFPVERFMRDAKI
                     TQIYEGTNQIQRVVIAQQLLGKS"
BASE COUNT         1118 a         2518 c         2573 g          938 t
ORIGIN      
        1 cgccgagccg gatccgcagc gaggagagga actcggccga gaggccgcgg ccgccctcgt
       61 agtggaggag gccggtggac gggtccaggc gcgagattcc gagggccgcg acccccagca
      121 gggcctggca gcgatcgacc acaagatcga gcacgcggtc gacctcgagg gtcgagctca
      181 ccaccgtgct gacctcgagc agggcctggt actgggccgc gttgcgggtg gcctcgtcga
      241 agaggcgcgc gtgctcgagg gccaccgcgg cctgggtcgc gaagtcggtg agggtcgaga
      301 aatcctcctc ggtgaagggc gccgcccccg gcccgcggcc cgcggtcatg acgccgaggg
      361 cccggccgcc cgccatcagc ggcgccgcga gcaggcggct ggtgaacggc acgaactcgg
      421 ccatcgcgtg ctgagaatcc gggtagtcgt tgaccatcac cccgcgcccg gtgtgcatcg
      481 cggcgccggc caccccgcgg tcggtggtga agcgcaggtc ccggacccag tcgccgatgt
      541 ccgaccacgc gcgcgggtgc agggtgtcgc cctcgagcat gtagagggcc gcgtagccgc
      601 cgccgatcag gcgctgggcc gcccgggcga tgaccaccag cagctgctcg gtcgcgagct
      661 gctcggagat gtggcgctcg atgagccgct gcgcctccag ccggctcaga cgagccgccg
      721 ccatgtcgcg caggcgtgca cgctcgaccg cgaggcccac ctgctgggcc acgctcgaga
      781 ggatcttcat ctcctcgtcc gagaactcgc gtgtctcgcg ggagacgagg gccatcacgc
      841 cccacgtctg ctcctccacc gggatcggca tcgcgagctg ggtgcggtgc gcccgctccg
      901 ccgccatctg gcggagccct tcctccgacg ggggcaccgc gtcgaggtgg gtcaccagga
      961 tctggttggt gcgcaccgcg tcgccgatgc gcgagccgtc caccgggcgc acccgcgacg
     1021 tctccacgtg acgcgggtcg aacccgctgc ccgcgaccat gatcagctcg cgcccggagg
     1081 cgtcgagctg gtagacgctg cccacgtcca tgcccgcgag actgcgcacc acctcgaggg
     1141 tctcgcgcgc ggtggtctcc agattcgcgc cgcggccgat cgtggtggcg atcgcgttca
     1201 gggcctcgag ctcgcggttg cgccgctcga gggcgatccg cgggttctcc gtccgcggtc
     1261 ctgcgaccgg ccgggcccgg cccgcggacg gcaggaaggc cagcaccaca ccggccagga
     1321 ccaccaggcc acccgcgagg agcgcgagga actcgaagag cagccgccgc tcggcctggg
     1381 cgaactcgcg caccggcaag gccacccaga gcgcgccgtc gggcgaggcc tcggccggga
     1441 agtcgaggcg gcggagggcc cacggctcgc cggccaccgt cgcgatcccg gatcccaccg
     1501 cgggcgtcca cccggtggcg ggcaggtccg cgcggctcgt cgcgagcgcc cggtcgccgg
     1561 ccaggaagag caccgcggga cgcgcgggca gccgcgcgag cgacgccgcc agcccgtcca
     1621 ggcgccggcc cgccaccacg gtgccggtgg caccgaacgg cgcgcgctcg gtcgcaccgc
     1681 tgatgggtgc caccaccagg aagtaggggc ggcccccggc caggcgcagg gccgtgatcg
     1741 gctcgctgga ggccggcatt ccggggaggc tcggcggcgg cccggccggc acctgtacca
     1801 gggacgcgcc gcggccatcg cggatcgcga tgaagtcggc gaggccgtcc tgcgtcaccg
     1861 ccagcacccg cggcgatgcg ccgcgcgcga gcgtggccca gtctcccttg gcggtgccct
     1921 cgatgatcgc cggatcgcgg gcgaggagca gggtctcccg cttgagcgcg tcctggtagt
     1981 cgccgacgag cgcgatcacc gcgtccacgg tccgcccgat cgcaccttcg cgggtgcgag
     2041 caagctgacg ctccgcgctg atgaatccga gcccggccag gatgcccagc gggacggcca
     2101 gcaccaggaa cgggatcgcg aagtggcgag tgagacgccc cacgagccta ttctcggccc
     2161 gcagggcgcc taaatgaaca ggtttttcgg gggcctagag gcccaggtag gcctgcttga
     2221 tgtgccggct ggccaggaga tcgtcccgcg ggccggacag cgtcatccgc ccgttctcga
     2281 ggacgtaacc ccggtgagcc aagtgtagag agcgcaatac gttctgctcc accaggagga
     2341 cggtgagccc ctgcccgttg atgcgcccca ggtcctcgaa gatggccttc accaggaccg
     2401 gggccaggcc caggctcggc tcgtccagca tgagcaggcg cgggcgggcc atgagcccgc
     2461 gcccgatggc gcacatctgc tgctcgcccc ccgagagcgt gccggccagc tgggcgcccc
     2521 gctcccggag gcggggaaac agctcgaaga cgcgctcgag ggtctcggcg cgcgagccgc
     2581 gggcggcgcg cgtgcgcgcg cccagctcca ggttctcccg caccgtcatg gtcgggaaga
     2641 gctggcggcc ctccggcacg tgcgcgatgc cgcgggccac gatctcggcc gggccgagcc
     2701 cgtccagccg ctggccctcg aactcgatcc ggccccggcg gagcgggagg agcccggaga
     2761 ccgcgcggag cgtggtggtc ttgcccgcgc cgttggcccc gatcagcgcc accgcctcgc
     2821 cctcgcgcac ctcgagcgag acgtcgcgga ccgcggtcag gtcgccgtag ccgacctcga
     2881 tgccgtcaac gcgcaggagc gtggacatat tcctcgccga ggtaggcctc gatgacgcgc
     2941 gggtggttgg cgatggcggc cggcgggccc tccacgatct tctcgccgaa gctcaggacc
     3001 acgatgcggt gggagaggct catgaccgcc cgcatgttgt gctcgatgag gaggatcgag
     3061 atgcccgagg cgtggatgcc ctggatcagc gccacgatcg cctcgatctc ggtcgggttg
     3121 aggcccgcca tcacctcgtc gagcagcagc agggtgggct cggtggcgag cgcgcgcgcc
     3181 agctcgagcc gcttgcgctc ggccagggtg agcccgcccg ccggctggcc cgccttggcc
     3241 cccagcccca cctggtcgat cacggcctcc gctcgccggc gcgcctccgc catctggggc
     3301 cgacgcgcga gcgcgcccac cctcacgttc tcccacaccg aaaggtgcgg aaaggggcga
     3361 acgatctgga atgtgcgggc caggccgagc cggcagatgg cgtgcggctt gagcccggcg
     3421 agcgatcggc cgtcgaagcg gacgtcgccg ccatccgggg ccaggaagcc ggagagcaga
     3481 tggaagaccg tggtcttgcc cgccccgttg ggcccgatga gccccagcac ctcgccgcgg
     3541 gccatgtcga acgagagatt tcgcaccgcc tgcaggccgc cgaaccgctt gccgattcca
     3601 cgaacctcga gcaggggcgg cgtgctcatc gggcggcctt cacccagaga cggcgcaggg
     3661 cgggatacgc cccctggggc aggaacagca ccaccgcgat gagcagcagg ccgtaggcga
     3721 tcaggtgggc gcccgaccag ctcccctgcc cgaagtagat ccgcgaggcc tccgcgagcg
     3781 ggctcaggat gaacgagccg aggatcgggc cgagcacggt ccctgccccg cccacgatcg
     3841 ggcggatcac gatctcgacc gagagcggga tgccgaacac cgagttcggc tggagcgaga
     3901 acaggtagaa ggcgtagaac gtccccccca ccccggtgag gaacgaggac accaccatcg
     3961 cgagcatctt gtagcggaag gtgttcaccc cgagcgcctc gcacgcctgc tcgtcctccc
     4021 ggatcgcgat caggtaggcc ccgaagcgac gccgctcgat ggcggccacg aggccggtgg
     4081 ccacgagcat caggccgagc gcgacgtagt agtagacgcg gttgtcctgg aactggaact
     4141 ggcgcgggtt gccggtgaac gtgatgtaga agccgagcgc gccgcccacc gcgtcggtgt
     4201 tgagcgccac gatgcggcag acctccgcga aggccaccgt caggaggacg aagtagaagc
     4261 cgcgcagccc gaaccggaag cccaggtagc cgaccacggt gccgagcacg gcggcgagca
     4321 cgccacccac cagcatcccg atccacgggg tgagcccggc ctgggcggag agcagcgcgg
     4381 aggtgtaggc cccgatgccg acgaaggcgg cgtgaccggc cgagagctgg cccgcgtagc
     4441 cccccacgat gttccaggcc tggccgagga acgcgtagaa gaagatgaag atgaacacgg
     4501 tgaccgcgta ggagttgagc cagagcggca atgcgagcag gagcgcgatc gcggccgcca
     4561 cgacccactt cacgacgcgg gccgcccgaa gaggccggcg ggacggaaga gcaggatgac
     4621 gatgagcagc gagaagctca ccagctcctt catcgacggg ctcaggaaca ccgcgccgag
     4681 cgactccgcc accgagacga tcaggccgcc caggaatgcg cccggcaggc tgcccatgcc
     4741 cccgatgatc acgatgttga acgaggcgac cgagagcccg agcccgcccg cgggctggaa
     4801 gggcacgatc ggcgacacca gcgcgcccgc cgcccccacg cacgccgccc cgatgccgaa
     4861 ggccaccgcg tagacccggc gcacgtcggc cccgatgacc cgggccccat aggcgttgtc
     4921 ggcggccgcc cgcatggtgc gcccgagatc ggtgcgggtc aggaagagat agagcgcgag
     4981 gctgaggagc atcgcgagcc cgaaggtgac cagccgggcc acgtccacga acaccgggcc
     5041 cagccagatg gtggcggcgc ggaggggcga gcggacccgc tggggatcgg ggccgaaggc
     5101 caccagcgcc gagttctcca gcaccagggc caccccgagc atcaggagga tctgcatctc
     5161 gtggggggcg tccacgacgc ggtcgaccag gacgcgctgg atcaccacgc ccagcacgaa
     5221 gagcgcggcc gcgcacgcca cgaacccgat gtacgggtcg atcccggccc gggtcgccag
     5281 gagccaggcg aggtacatcc cccacatcat catgtcgccg tgggcgaagt tcaccacccg
     5341 catcaccccg aagatgaggg tgagccccac cgccatgagg ctgtagacac ctccgaacag
     5401 gagcccgctg agcaggccct gagcgatgag cgccgggtcc aggggcggct acgccttggg
     5461 acgcgggaag acgaacttct ggatcgcggc ctccttcggc cagaccgcga cgggcttgcc
     5521 gccgagaatc tggagcatgg tggtcaccgc gttcggattg tcgccgatct cgttgaagtg
     5581 cacgggaccc gcatactgca tgaggccggc cgtccagttc gtcttgtgga tcgcctccac
     5641 gatcgcgtcg ggatcggtgg acttggcccg ctcgagcacg tccgcgatca ggctgacccc
     5701 gtcgtaggag tacccggagt tcgtatcgaa ggtcttgccc tgggagcgct tgaggtactc
     5761 ctcggccacc ttctgggtcc gcgcgttctt gaagttggcc cagggcgtgc tgttcagcac
     5821 gtactccaga tcctccttga gcagcgcgat ctggccggcc tcgtagagcc ccgggctgcc
     5881 cgggcccacg atgcccatga tctcgaggcg ctgcttgcgg atctcgggca gcagcagctg
     5941 cgcgctcgcc ggccgggtga tcggcgcgat gatgtcgggc ttggccgcct tcgcccgcga
     6001 cacctcggtg gagaggtcct ggggcggctc gggccatgga atcacgtcga cgatctccca
     6061 gcccggcttg gccgcggcgt gggcggcctg gaagcccttg gcgttgttct ggccgaagag
     6121 gtcgttgcag tacatcagca ccactcgctt cggcgagacc ttggcttcct ggaagatctc
     6181 ggtgaagtac tggaccgcct tgcggccgaa cgaggagccg gtggggaagt tccggtagac
     6241 gtactggacc ttctgctgtc cctccttcac cgccttcgcc acgttcgcgg tgatcggatc
     6301 ggcggccgcg atgtcgacca ggaacgggac gcggcgctgc tgggccaccg ggacgatcgc
     6361 ctgggtcgag ccggagtcga aggagcccat cagcatctgg gcgccctggt tgatcacccg
     6421 ctccgcctcg gtccgtccga tatccggttt cgtctgggtg tcgcccacga tgaggtcgat
     6481 cttcatgccg ttgagcgact tgatcccccc cgccgcgttg atggcgtcgg ccgccatctg
     6541 cgcgccgagc cggcaggcct gaccgggctc cgcgaggggg ccggtcaccg ggtggatggc
     6601 ccccaccttg aaggtcggga cctgggcgcg cagcaccgcc gggaagccca gggcggcggc
     6661 gccggcggcg gtcccggcgg ccttcagacc cgcctttagg aacgtgcgac ggtgcactcg
     6721 ggacgggcct cggcgatcgc tcatcgactc ctccgtgatg gaaagacggt caggacttgc
     6781 ccaggagctg ctgggcgatg accacgcgct ggatctggtt cgtgccttcg tagatctggg
     6841 tgatcttggc gtcgcgcatg aagcgctcca ccgggaactc gcgggtgtag ccgtagccgc
     6901 cgaagatctg caccgcgtcg gtggtcacct tcatcgcggt gtcggaggcc aggcacttcg
     6961 ccaccgaggc ctcgagcgcg gtgccggtga tcccgtggtc gatgaggccg gccgcgtggt
     7021 gcacgaccag gcgcgaggcg tggaccgcga tcgccatgtc cgcgagcatg aactggagcc
     7081 cctggaactg ggcgatgggc tgatcgaacg cccgccgctc cttcgcgtag gccaccgacg
     7141 cgtcgag
//
LOCUS       DATMCV010000423         3579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_958839, whole genome shotgun sequence.
ACCESSION   DATMCV010000423 DATMCV010000000
VERSION     DATMCV010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958839"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VNU03_09825"
     CDS             <1..136
                     /locus_tag="VNU03_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWP74804.1"
                     /translation="YIYDVGFKFWDQAYAAALTLVLLVILAAVALGQFLLLDRRVHYQ
                     "
     gene            133..933
                     /locus_tag="VNU03_09830"
     CDS             133..933
                     /locus_tag="VNU03_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWP74805.1"
                     /translation="MSRALETAGAWLLGVLWALPLAYAVWTAFHPAEFSTRFVPGAPL
                     TLENFAKAWAAAPFARYFVNTTLLVAMILAVQLVLCTLAAYAFARFAFAGRGVAFLLV
                     LVQLMIMPDVLIVENYRTMSRLGILDTLWAIGLPYMASAFGIFLLRQTFKTVPKELDE
                     AARVEGAGPLETLWRVYVPLARPVYIAYGLVSVSYHWNNFLWPLIVTNSVQSRPLTVG
                     LQVFSSVDQGVDWSIITAATLMTSGPLLVAFLLFQRQFVQSFMRAGIR"
     gene            complement(950..2929)
                     /locus_tag="VNU03_09835"
     CDS             complement(950..2929)
                     /locus_tag="VNU03_09835"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HWP74806.1"
                     /translation="MATTRRICAGLLVFAILLAGVAPVRAGSSAPPPPAPAAPPAPAP
                     QAAPAAPAESRAEEPAEATPPRVSYLHGDVSLWRPGAQDWTAAALNTPLAPGDVLYTG
                     PAGNVEIQVGPRAFVRAAYGAQIGLDNQEPDFVQLRVTSGYAALDLRELQPGHAVELD
                     TPGGAFTMERAGYYHAEVSADTTTFRTHRGGTATMTPSGGAAAPVAANQQVVISGGAS
                     PRVETGAAAPLSSWDRWNYQRTDYLIQPASAKYVPAGVYGAEALDQHGSWRSVESYGS
                     VWVPAGVPSGWSPYTTGHWIWDPRFGWTWLDDAPWGWAPYHHGRWVFVGPYWAWAPGP
                     VLVRPVYSPALVVFLGGPVVVGRPVYWAPLGWGEPVIRWWGGPRFVGVAWWGGWGGPR
                     VVNNVVIARTATVNVTNITVYHNVNVTNAVVGVPAGRFGQAGVRAARLTDAAEVRQLA
                     PVRGALEVRPVAASLTAGAGPAASRPPEAVRSRAVVATRAPRDFSPELRAEGLAAATP
                     ATSPQPQQRIVPAPARTVTPQAAPGGSGAPRAAGGAGRAASEDGPRRERGRGGAERGA
                     GQPAPPGGAAREAAPSRQAVPPAPPAPGVQSAPGGQPAATGQAEPKARRQPAEHPGRA
                     EGPARGGNPERGAVERGGGERGGGEKGGARGERDR"
     gene            3029..3373
                     /locus_tag="VNU03_09840"
     CDS             3029..3373
                     /locus_tag="VNU03_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74807.1"
                     /translation="MKKTLLVLLLVVGVLALTVAPGYTWGHWRGGVFVGVGPYWGPYP
                     YYYYPPPPPYYYSPPTVVIEQPPVYVEQPAPPPAPQAPTAFWYYCSSAKGYYPTVASC
                     PEEWVKVPERPR"
BASE COUNT          423 a         1333 c         1304 g          519 t
ORIGIN      
        1 ctacatctac gacgtgggct tcaagttctg ggaccaggcc tacgcggcgg ccctcaccct
       61 ggtcctgctc gtgatcctgg ccgcggtggc cctggggcag ttcctgctgc tggaccggcg
      121 ggtgcactat caatgagtcg cgcgctcgag acggcgggcg cgtggctgct cggggtgctc
      181 tgggcgctgc cgctggccta cgcggtgtgg accgccttcc acccggccga gttctcgacg
      241 cgcttcgtcc ccggcgcgcc gctcaccctc gagaacttcg cgaaggcgtg ggcggccgcg
      301 cccttcgccc gctacttcgt caacaccacg ctgctggtgg ccatgatcct ggccgtccag
      361 ctcgtgctct gtacgctcgc ggcctacgcc ttcgcgcgct tcgcgttcgc ggggcgcggg
      421 gtcgcgttcc tgctggtgct ggtacagctc atgatcatgc cggacgtgct gatcgtggag
      481 aactaccgga ccatgagccg gctcggcatc ctcgacacgc tctgggccat cggcctgccc
      541 tacatggcct ccgcgttcgg catcttcctc ctgcgccaga ccttcaagac ggtgccgaag
      601 gagctggacg aggcggcgcg ggtggaaggc gcgggaccgc tcgagacgct ctggcgcgtc
      661 tacgtgccgc tcgcgcggcc ggtctacatc gcctacgggc tggtctcggt gagctaccac
      721 tggaacaact tcctgtggcc gctgatcgtc acgaactcgg tgcagtcgcg gccgctcacc
      781 gtggggctac aggtgttctc ctcggtcgat cagggggtgg actggtcgat catcaccgcg
      841 gcgaccttga tgacctcggg cccgctgctg gtggcgttcc tgctcttcca gcgacagttc
      901 gtgcagagct tcatgcgcgc cggcatccgc tgaccgcggc ccgcgccgcc tagcggtcgc
      961 gctcgccgcg cgcgccaccc ttctcgccgc cgccgcgctc gccaccgccg cgctcgaccg
     1021 cgccgcgctc cgggttgccg ccgcgcgccg gtccctcggc gcgcccggga tgctccgccg
     1081 gctggcgacg ggccttcggc tcggcctgcc ccgtcgcggc cggctggccc ccgggggcgc
     1141 tctggacgcc gggggcgggt ggcgcgggcg gcaccgcctg gcgcgacggc gccgcttctc
     1201 gcgcggcgcc gcccggcggc gcgggctggc ccgcgccgcg ctccgccccg ccgcggcctc
     1261 gctcgcgacg cgggccgtcc tcggacgcgg cgcgtccggc tccaccggcg gcgcgcggcg
     1321 ctccgcttcc gcccggcgcg gcctgcggcg tcacggtgcg cgcgggcgcg ggcacgatgc
     1381 gctgctgcgg ctgcggtgag gtggcgggcg tcgcggcggc caggccctcg gcccgcagct
     1441 ccggcgagaa gtcccgcggt gcgcgcgtcg ccacgaccgc acgtgagcgc accgcctccg
     1501 gcggccgcga cgccgccggc cccgcgccgg cggtgaggct cgcggcgacc ggccgcacct
     1561 cgagcgcgcc gcgcaccggg gcgagctggc gcacctcggc ggcatccgtc agacgcgcgg
     1621 cccggacgcc ggcttgaccg aagcggccgg ccggcacgcc caccaccgcg ttggtcacgt
     1681 tcacgttgtg atagaccgtg atgttggtga cgttgacggt ggcggtgcgg gcgatcacca
     1741 cgttgttgac cacgcgcggc ccgccccagc ctccccacca ggccacgccc acgaagcgcg
     1801 gcccgcccca ccagcggatc accggctcgc cccagccgag gggagcccag tagaccgggc
     1861 gaccgaccac gaccgggccg ccgaggaaga ccacgagcgc gggcgagtag accgggcgga
     1921 ccagcaccgg gccgggggcc cacgcccagt acgggccgac gaagacccag cggccgtgat
     1981 ggtacggcgc ccagccccac ggggcgtcat cgagccacgt ccagccgaag cgcggatccc
     2041 agatccagtg cccggtcgtg tagggcgacc agcccgacgg cacgccggcc ggcacccaga
     2101 cgctgccgta gctctcgacg ctccgccagc tcccgtgctg gtcgagcgcc tcggccccgt
     2161 agacgcccgc gggaacgtac ttggcgctcg cgggctggat caggtagtcg gtgcgctggt
     2221 agttccagcg gtcccaggag ctgagcggcg cggcggctcc tgtctccacg cgaggcgatg
     2281 cgccgcccga gatcaccacc tgctggttcg cggccaccgg ggcggcggcg ccgcccgacg
     2341 gtgtcatggt cgcggtgccg ccccgatgcg tgcggaacgt ggtcgtgtcc gccgagacct
     2401 ccgcgtggta gtagccggcg cgctccatcg tgaacgcgcc gccgggcgtg tcgagctcca
     2461 ccgcgtgccc cggctggagc tcgcgcaggt ccagcgcggc gtagccggag gtgacgcgga
     2521 gctgcacgaa gtccggctcc tggttgtcga ggccgatctg ggcgccgtag gcggcgcgca
     2581 cgaaagcgcg cggtcccacc tggatctcca cgttgccggc cgggcccgtg tagaggacat
     2641 cgcccggggc cagcggcgtg ttgagtgcgg cggcggtcca gtcctgcgct cccggccgcc
     2701 agagggagac gtcgccgtga agatagctga cgcgcggcgg cgtagcctcc gccggctctt
     2761 ctgcgcgact ctccgccggc gccgccgggg ccgcctgagg cgcgggggcg gggggtgccg
     2821 ccggggccgg cggcggtggt gcgctactcc ccgcccggac gggcgcgacg cccgccagga
     2881 gaatcgcgaa gaccagcaat cccgcgcaga tccgtcgtgt cgttgccatc gtatgatcat
     2941 cgtaacagat ggcgtccggg gcctcgggag gatatgcttg gaacgatgta aacgaagtcg
     3001 gatcgacgct cgtcataacg aggcgacgat gaaaaagacg ctcttggtcc ttctgctggt
     3061 cgtcggggtt ctcgcgctca ccgtcgcccc tggctacacc tggggccact ggcgcggcgg
     3121 agtgttcgtc ggggtcgggc cctactgggg cccttacccc tactactact acccgcctcc
     3181 gcccccgtac tactattcgc cccccacggt ggtgatcgag cagccgccgg tctacgtcga
     3241 gcagcccgcg ccgcctccgg cgccgcaggc gcccaccgcg ttctggtact actgctcgag
     3301 cgcgaagggc tattacccga cggtggcgag ctgcccggaa gagtgggtga aggtgccgga
     3361 gcggccgcgg tgagtcggtt gacaccatca cgcaccggcg cctccgcgcc gatgccgggc
     3421 ccggcgctcg cgtccgtcat cggggcggtg atcgtcgcgc tggtggtgag cgggtgcgcc
     3481 agcgtcccga ccgggccgag cgtgatggtc atgccgggca ccggcaagag cttcgagacc
     3541 ttccaggtcg acgacgcggt atgccgccag tgggcggcg
//
LOCUS       DATMCV010000424         2751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_555302, whole genome shotgun sequence.
ACCESSION   DATMCV010000424 DATMCV010000000
VERSION     DATMCV010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555302"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..755
                     /locus_tag="VNU03_09845"
     CDS             189..755
                     /locus_tag="VNU03_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP74808.1"
                     /translation="MANKGFSHIGLSTLDLDRTRDFYENVLGFKAVRCDVLRVKEGGQ
                     IRHIFFDTGRDQLIAFMEARGVPGVPAAYDAGINRGLGVPSVFYHFAFEAGSEAALQD
                     KRDELLARGVEVTEIVDHDWAKSIYFKDPNGLQLEFCSFTRNLNADDARLQDRAEISV
                     RRLGLQDPDALRREVPAGAVMAATADRP"
     gene            complement(752..1048)
                     /locus_tag="VNU03_09850"
     CDS             complement(752..1048)
                     /locus_tag="VNU03_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWP74809.1"
                     /translation="MIRVILPAHLRTLARVDGEVKVEVAGPITVGAVLDALEAAYPML
                     RGTIRDQVTRQRRAFLRFFACEEDLSHDPPDTPLPEAIASGAEPLLVVGAMAGG"
     gene            complement(1056..2171)
                     /locus_tag="VNU03_09855"
     CDS             complement(1056..2171)
                     /locus_tag="VNU03_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HWP74810.1"
                     /translation="MSRVRVLVGTRKGAFVLTADAKREQWDVTGPHFGGWEIYHLKGS
                     PADPDRLYASQSSGWFGQVIQRSSDGGRTWETVGNKFSYDGATGTHQWYDGTPHPWEF
                     ARVWHLEPSLTDPDTVYAGVEDAALFRTTDGGQSWHELSGLRTHTSAPSWQPGAGGMC
                     LHTILLDPSRPGRIFVAISAAGAFRSDDDGATWRPINRGLRSEGIPDPHAEVGHCVHR
                     IAMHRSRPGVLFMQKHWDVMRSDDAGDSWREVSGGLPSDFGFPIDVHAHDPETIYVVP
                     IKSDSEHYPPDGKLRVYRSRTGGNEWEALTSGLPQRHCYVNVLRDAMAVDSLDPCGVY
                     FGTTGGQVYASADGGDRWAPIVRDLPSVVSVEVQTLP"
     gene            2374..2748
                     /locus_tag="VNU03_09860"
     CDS             2374..2748
                     /locus_tag="VNU03_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWP74811.1"
                     /translation="MTYALWILQILLGLLFLFSGSMKFVMSVAEMTKDMPSMPGWFLH
                     FIGVMEILGGLGLILPSLLRIRPGLTPLAAAGLVIIMSGATAITLSTMGVGPAVLPLI
                     VGILCAFVAYGRWRVAPLSARG"
BASE COUNT          408 a          969 c          925 g          449 t
ORIGIN      
        1 actgtcgatc cgggcgatct ccttcatccc gaacacgttg acgtagaagg cggcggtctt
       61 gtccacgtcc tgggtcgaca gcgcgatgtg cttgatcttg gccatggaat cctcctcggg
      121 gggctcgtgt ggcgaatgat accacggcgc ggtactgtac ccgctgtacc cctcaaggag
      181 ggcatcccat ggccaacaag ggcttttctc acatcggtct ctccaccctc gacctcgacc
      241 ggacccggga tttctacgag aacgtcctgg gcttcaaggc ggtgcgctgc gacgtgctcc
      301 gggtgaagga gggcgggcag atccgccaca tcttcttcga caccgggcgc gaccagctca
      361 tcgccttcat ggaggcgcgc ggggtgccgg gggtaccggc ggcctacgac gcggggatca
      421 accgggggct cggggtgccg agcgtgttct accacttcgc cttcgaggcg ggctccgagg
      481 ccgcgctcca ggacaagcgc gacgagctgc tcgcccgggg cgtcgaggtc accgagatcg
      541 tcgaccacga ctgggccaag tccatctact tcaaggaccc gaacgggctg cagctggagt
      601 tctgcagctt cacccgcaac ctgaatgcgg acgacgcccg gctgcaggac cgcgccgaga
      661 tctcggtccg acggctcggc ctccaggacc ccgacgcgct ccggcgcgag gtgccggccg
      721 gcgcggtgat ggcggcgacg gcggaccggc cctagccgcc cgccatcgcg cccaccacca
      781 ggagcggctc cgcccccgag gcgatcgcct cgggcagcgg ggtatccggg gggtcgtggg
      841 agaggtcctc ctcgcacgcg aagaaccgga ggaaggcccg ccgctgccgc gtgacctgat
      901 cgcggatcgt cccgcgcagc atggggtagg ccgcctccag cgcgtcgagg accgcgccca
      961 ccgtgatcgg ccccgcgacc tcgaccttca cctcgccgtc cacccgcgcg agcgtccgga
     1021 gatgagcggg caggatcacg cggatcatgg gtggctcacg gcagggtctg gacctcgacg
     1081 gacaccaccg aggggaggtc ccggacgatg ggcgcccagc gatccccccc gtcggccgac
     1141 gcgtagacct gaccgccggt ggtgccgaag tacaccccgc atggatccag cgagtcgacc
     1201 gccatcgcgt cgcgcagcac gttgacgtag cagtggcgct gcgggaggcc gctggtcagc
     1261 gcctcccact cgttcccgcc ggtccggctg cgatagaccc gcagcttgcc gtccggcggg
     1321 tagtgctccg agtcgctctt gatgggcacc acgtagatgg tctccgggtc gtgggcgtgc
     1381 acgtcgatcg ggaacccgaa gtcgctcggc aggccgccgc tcacctcccg ccacgaatcg
     1441 cccgcgtcgt cgctgcgcat cacgtcccag tgcttctgca tgaacagcac ccccggccgc
     1501 gagcggtgca tcgcgatgcg atggacgcag tgccccacct cggcgtgcgg atccgggatc
     1561 ccctccgacc ggaggccgcg gttgatcggc cgccacgtcg cgccgtcgtc gtcgctccgg
     1621 aacgcgcccg ccgcggagat cgcgacgaag atccggccgg gccggctcgg gtcgaggagg
     1681 atcgtgtgca ggcacatccc gcccgcgccc ggctgccagg acggcgcgga ggtgtgggtg
     1741 cgcagtccgg atagctcgtg ccagctctgg ccgccgtcgg tggtgcggaa gagagccgcg
     1801 tcctcgaccc cggcgtagac cgtatccgga tccgtcaggg agggctccag atgccagacc
     1861 cgcgcgaact cccaggggtg gggcgtcccg tcgtaccact ggtgggtccc ggtggccccg
     1921 tcgtacgaga acttgtttcc caccgtctcc cacgtccggc cgccgtcgct cgagcgctgg
     1981 atcacctggc cgaaccagcc actggactgc gaggcataga ggcgatccgg gtccgcgggc
     2041 gatcccttga ggtgatagat ctcccatccc ccgaagtggg ggccggtcac gtcccactgc
     2101 tcgcgcttgg cgtcggccgt caggacgaac gcgcccttgc gcgtgccgac cagtacccgt
     2161 accctgctca ttcgtcgctc ctttgccggg ccccccatcg cgcgaggtgt agtcccggcg
     2221 gcatgaattg atttctacta tatggtcgta ccggccggcc cgccatcgac aggccggccg
     2281 aacaatcgga tcgacgatcc gtgtcgattc caggactccc gggacgacta cccccgaaac
     2341 caacgtgtac tcaccttgtg gaggagatgg atcatgacct atgcgctctg gattcttcag
     2401 atattgctgg gacttctgtt cctgttctcc ggcagcatga agttcgtcat gtcggtcgcg
     2461 gagatgacaa aggacatgcc atcgatgccg ggctggttcc tgcacttcat cggggtgatg
     2521 gagatcctcg gggggctcgg cctgatcctg ccgagcctcc tgcgcatccg gcccgggctg
     2581 acccccctcg ccgcagcggg cctcgtcatc atcatgagcg gggcgacggc gatcacgctc
     2641 agcacgatgg gagtcggccc cgcggtgctc ccgctgatcg tcgggatcct ctgcgccttc
     2701 gtcgcctacg gtcgatggcg agtggccccg ctgtcggccc gcggctgatg c
//
LOCUS       DATMCV010000425         4285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_489904, whole genome shotgun sequence.
ACCESSION   DATMCV010000425 DATMCV010000000
VERSION     DATMCV010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489904"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1317)
                     /locus_tag="VNU03_09865"
     CDS             complement(31..1317)
                     /locus_tag="VNU03_09865"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP74812.1"
                     /translation="MLARDDNERVTRVGPGTPMGATMRRYWLPALLGWELPEPDCAPV
                     RVKLLGEELVAFRDSRGRIGLLDERCPHRQASLFLGRNEECGLRCVYHGWKFDADGQC
                     VDMMNEPPELAFAAKIRTGAYPTVEAGGIIWAYLGPAACKPPLPHFAWTQAPPTHRHV
                     SKVIQESNWLQGLEGGIDTSHVPILHRVLATGSARPGFTPADPLVRGGAPTLELDVTD
                     YGYRYAGLRPLADGELHVRAYHFILPFHQIRPARTGSGARGFSGAAGHIWVPMDDETT
                     MVYNWEHSTTEPLTDEDRLERRLGNGPLDVDQTTFRSKRNWENNYLLDRRVQKTETFT
                     GIDGVNAQDRAVQESMGSVVDRTREHLGPADRAVIHARRLLLQATRTVEAGGTPPGVE
                     GTYYPLHPAEAVVPKDTDWRTLLASGAPRSDVLQRA"
     gene            complement(1352..1600)
                     /locus_tag="VNU03_09870"
     CDS             complement(1352..1600)
                     /locus_tag="VNU03_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74813.1"
                     /translation="MDDRRPLAYYEAVPYLLVLESVERGGEWLRRAEHPELPDCVAEA
                     HSAVEALDRLERERLRVLRQMWDRGAAIPVPRPPLRQP"
     gene            complement(1600..2901)
                     /locus_tag="VNU03_09875"
     CDS             complement(1600..2901)
                     /locus_tag="VNU03_09875"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP74814.1"
                     /translation="MLSSEDNELLCRVGRGTPMGDLLRHYWMPCLPAAELPAPDCPPK
                     KVRLLGEDLVAFRDSRGEVGLLAANCPHRGASLFFGRNEECGLRCSYHGWKYDVTGRC
                     VDMPNEPEESTFKDKIRARAYPCRDVNGVIWTYMGPRETAPPLPAFEINTLPAAQVYP
                     PLMMLEECNWVQALEGDIDSSHIDFVHAKRTPDSRQRGTFHRDKRPRLEVLPTDYGAC
                     YSARRRSDVEGLYWHRITQFILPFYSMIAASDPHIVSARAWVPLDDHYNLQFVMRARL
                     DRPVTEEEARQVRDPFASWGGYVESDGDPRRRYYTAANIHNDYRQDHELKKELTLGIP
                     FLGNLQDRAMTETMGTIYDRTQEHLGTTDAMVILVRRRLIEAARALRSQGALPPNVDD
                     PNLCRVRPASAVLPDGASWISATEKARQSDAGVPIAWVPFV"
     gene            complement(2923..3885)
                     /locus_tag="VNU03_09880"
     CDS             complement(2923..3885)
                     /locus_tag="VNU03_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HWP74815.1"
                     /translation="MAQITLGIGSSHSPMLSTPHEAFAGLGDLDRSRLPEFATKAREC
                     AAWIGRELAAEVTRARHEATQAAIRQLGAVLAEEAPDAVVVIGDDQNEWFGPDNQPAL
                     CIYWGESVDNLPPPIEKVPPLRRLSYWGLYGDGANRTFPVDAALGRHLVESLTRDFDF
                     DVAHLRVQPRHGPLGHAWSFVHQRLMGDRVVPVVPVLLNTYYPPNQPTPRRCMQLGRA
                     IRQAIEAWPIHKRVAVVASGGLSHFFVDEELDRHVLDLLAKKDGDALGALPAAQLESG
                     NSEIRNWIATAGAVEHLAMRLVDYVPSYRSEAGSGVGMAFAVWQ"
     gene            complement(3888..>4285)
                     /locus_tag="VNU03_09885"
     CDS             complement(3888..>4285)
                     /locus_tag="VNU03_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74816.1"
                     /translation="VGAFRAVHFGKTEAEAVALLRDTNYSGFQHYFGGFGFWEAFRTP
                     EDAAKYPLDPYTPLPPAEWTVDRMRKVKYALAGTVDQIKREVEALQKIGGQGDLEWFG
                     WFFDQGFMSWDEEERQMELFAKHIIPAFR"
BASE COUNT          586 a         1484 c         1567 g          648 t
ORIGIN      
        1 gaccgggcgc gtggcctcga agatttccgg ctacgcccgc tgcagcacgt cgcttcgcgg
       61 cgcgccggag gcgaggaggg tccgccagtc ggtgtccttc ggcaccaccg cctccgccgg
      121 gtgcagcgga tagtaggtcc cctcgactcc cggcggggtc ccgccggcct ccaccgtgcg
      181 ggtcgcctgc aggagcagcc ggcgcgcatg gatcacggcg cggtcggccg ggcccaggtg
      241 ctcgcgggtg cgatcgacga cggaccccat gctctcctgc accgcgcggt cctgggcgtt
      301 gacgccgtcg atgccggtga aggtctcggt cttctgcacc cgccggtcca gcaggtagtt
      361 gttctcccag ttgcgcttcg agcggaacgt cgtctggtcg acgtcgagcg gaccgttgcc
      421 gagcctccgc tcgagccggt cctcgtcggt gagcggctcg gtcgtgctgt gctcccagtt
      481 gtagaccatc gtggtctcgt catccatcgg cacccagatg tggccggccg cgccggagaa
      541 cccgcgcgcc cccgagccgg tgcgggccgg gcggatctgg tggaagggca ggatgaagtg
      601 gtaggcgcgc acgtggagct cgccgtccgc gaggggccgc aggccggcgt agcggtagcc
      661 gtagtccgtg acgtcgagct ccagcgtggg cgccccgccg cgcaccaacg gatccgccgg
      721 ggtgaaaccg ggccgcgcgg agccggtcgc gagcacgcgg tgcaggatgg gcacgtgcga
      781 ggtgtcgatg ccgccctcga gcccctggag ccagttgctc tcctggatca ccttcgagac
      841 gtggcggtgc gtgggcggcg cctgggtcca ggcgaagtgc gggagcggag gcttgcacgc
      901 agcaggcccg agataggccc agatgatgcc gcccgcttcc acggtcggat aggctccggt
      961 gcggatcttg gccgcgaagg ccagctccgg cggctcgttc atcatgtcca cgcactggcc
     1021 gtcggcgtcg aacttccagc cgtggtacac gcagcgcagc ccgcactcct cgttgcgccc
     1081 gagaaagagc gaggcctggc ggtgcgggca gcgctcgtcg aggagcccga tacggccgcg
     1141 gctgtcgcgg aaggccacga gctcctcgcc caggagcttc acgcgcacgg gcgcgcagtc
     1201 gggctccggc agctcccacc cgagcagcgc gggcagccag taccgtcgca tcgtggcgcc
     1261 catcggggtg ccggggccga cgcgggtcac tcgctcgttg tcatcgcgtg ccagcatgga
     1321 gggtctcctt ggggcaccgg gcctagcgtc gctagggttg ccggagcggt ggccgcggaa
     1381 cggggatggc ggcgccgcga tcccacatct gccgcagcac ccgcagccgc tcccgttcca
     1441 gccggtcgag ggcctcgacc gcggagtgcg cctcggcgac gcagtcgggc agctccggat
     1501 gctcggcgcg ccggagccat tcgccgccgc gctcgaccga ctcgagcacg agcaggtacg
     1561 gcaccgcctc gtagtaggcg agcggcctcc gatcgtccat cacacgaacg gcacccacgc
     1621 gatgggcacg cccgcgtcgg actggcgcgc cttctcggtc gcgctgatcc agctcgcgcc
     1681 gtcgggcagg accgcggagg ccggccgcac ccggcagaga ttcgggtcgt cgacgttggg
     1741 gggcagcgcg ccctggctcc gcagcgcccg cgcggcctcg atgagccggc gccgcacgag
     1801 gatcaccatg gcgtcggtgg tgcccaggtg ctcctgcgtc cggtcgtaga tcgtccccat
     1861 cgtctcggtc atggcgcggt cttgcaggtt gccgaggaac gggatgccga gggtcagctc
     1921 cttcttcagc tcatggtcct gccggtagtc gttgtggatg ttggccgcgg tgtagtagcg
     1981 gcggcgcgga tcgccgtccg actcgacgta gccgccccag gaggcgaagg gatcgcgcac
     2041 ctggcgcgcc tcctcctcgg tcaccgggcg gtcgaggcgc gcgcgcatca cgaactggag
     2101 attgtagtgg tcgtcgagcg gcacccaggc ccgcgcggag acgatgtggg gatcgctcgc
     2161 cgcgatcatc gagtagaacg gcaggatgaa ctgggtgatg cggtgccagt acagcccctc
     2221 gacgtccgag cgccggcggg ccgagtagca cgcgccgtag tcggtgggca gcacctcgag
     2281 gcgcggccgc ttgtcccggt ggaacgtgcc gcgctgcctg gagtcgggcg tgcgcttggc
     2341 gtgcacgaag tcgatgtggg acgagtcgat gtcgccctcg agcgcctgca cccagttgca
     2401 ctcctccagc atcatgagcg gcgggtagac ctgcgccgcc ggcagggtgt tgatctcgaa
     2461 ggccggcaag ggcggcgccg tctcgcgcgg ccccatgtag gtccagatca cgccgttgac
     2521 gtcgcggcac ggatacgcgc gggcgcggat cttgtccttg aaggtggact cctccggctc
     2581 gttcggcatg tccacgcagc gcccggtgac gtcgtacttc cagccgtggt acgagcagcg
     2641 gagcccgcat tcttcattgc ggccgaagaa gagcgacgcg ccgcggtgcg ggcagttggc
     2701 ggccagcagc ccgacctcgc cccggctgtc gcggaacgcg accaggtcct cgccgagcag
     2761 gcgcaccttc ttcggcgggc agtcgggcgc gggcagctcg gccgcgggca ggcacggcat
     2821 ccagtagtgg cgcaggaggt cgcccatcgg cgttccgcgt cccacccggc acagcagctc
     2881 gttgtcctcg ctcgagagca tggcgatctc ctcttgcgtt tcctattgcc agaccgcgaa
     2941 ggccatcccg acgccggagc cggcctcgga ccggtagctc gggacgtagt ccacgagacg
     3001 catcgccagg tgctcgaccg cgccggccgt cgcgatccag ttgcggatct cggagttccc
     3061 ggactcgagc tgcgccgccg gcagcgcgcc gagcgcgtcg ccgtccttct tggccagcag
     3121 gtcgaggacg tggcggtcca gctcctcgtc gacgaagaag tggctgagcc cgcccgaggc
     3181 cacgaccgcc actcgcttgt ggatgggcca ggcctcgatc gcctgccgga tggcgcgccc
     3241 gagctgcatg caccgtcgcg gcgtcggctg gttgggcggg tagtacgtgt tgagcaggac
     3301 cggcacgacg ggcaccacgc ggtcacccat gagccgctgg tgcacgaagc tccaggcgtg
     3361 gccgaggggg ccgtggcgcg gctgcacgcg caggtgggcg acgtcgaagt cgaagtcgcg
     3421 cgtgagcgac tccaccaggt gccggccgag ggccgcgtcg acgggaaacg tccgattcgc
     3481 cccgtcgccg tagagacccc agtaggagag ccggcgcagg ggtggcacct tctcgatcgg
     3541 cggcggcagg ttgtcgaccg actcgcccca gtagatgcag agcgcgggct ggttatcggg
     3601 gccgaaccac tcgttctggt cgtcgccgat cacgacgacc gcgtcgggcg cttcctcggc
     3661 cagcacggcg cccagctgcc ggatggcggc ctgcgtcgcc tcgtggcggg cgcgggtcac
     3721 ctcggcggcg agctcgcggc cgatccaggc cgcgcactcg cgcgccttcg tggcgaactc
     3781 cggcaggcgg gagcggtcaa gatcgccgag gcccgcgaac gcctcgtggg gcgtgctcag
     3841 catggggctg tgcgagctgc cgatgccgag ggtgatctgc gccatgccta gcggaacgcg
     3901 gggatgatgt gcttggcgaa gagctccatc tgccgctcct cctcgtccca cgacatgaac
     3961 ccctgatcga agaaccatcc gaaccactcg agatcgccct ggccgccgat cttctgcagg
     4021 gcctcgacct cgcgcttgat ctggtccacc gttccggcca gcgcgtactt gaccttgcgc
     4081 atccggtcga ccgtccactc cgccggcggc aggggcgtgt acgggtccag cggatacttc
     4141 gcggcgtcct cgggcgtgcg gaaggcctcc cagaagccga agcccccgaa gtagtgctgg
     4201 aagcccgagt agttggtgtc gcggagcagg gccaccgcct cggcctcggt cttgccgaag
     4261 tgcacggcgc ggaacgcgcc cacgc
//
LOCUS       DATMCV010000426         7790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_555361, whole genome shotgun sequence.
ACCESSION   DATMCV010000426 DATMCV010000000
VERSION     DATMCV010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555361"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3152)
                     /locus_tag="VNU03_09890"
     CDS             complement(<1..3152)
                     /locus_tag="VNU03_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP74817.1"
                     /translation="MNCPRCNAENRPDRRFCGACGLSFVSRCLACGFSNEASERFCGG
                     CGTALAPAAPGGGTRFGPPQSYTPKHLAARILDSKAALEGERKLVTVLFADLQSSMEL
                     LADRDPEEARRVLDPVLQLMMAAVHRYEGTVNQVMGDGIMALFGAPLAHEDHATRGCY
                     AALQMQESIKRYAEEVRQSLGVAVRIRVGLNSGEVIVRTIRSDLRMDYTAVGQTTHLA
                     SRMEQLADPGRILLTPGTLALAEGFIQVTPLGPTPVKGLAGPVEVYELTGAGPARSRL
                     RASATRGLTRFVGRTTEMAHLIGAMGRARAGHGQLVAVLGEAGVGKSRLCYEFTHSPD
                     AREWVVLETGAVSYGRGTSYSPVIDLLKQYFGIEGRDDHPRVREKVAEKLMALDPALA
                     RYHAALLWLLDVPSDDPHWADLDPTQRRRRALDGVRMLLVRESARRPVLLLCEDLHWI
                     DTETQTLLDEVVASLPTARLLLVVTYRPEYEHGWANISVCTQFRVEALPVETTDELLE
                     TLLGRDPGLRPLKRLLVQLTDRNPFFLEETVRTLSEVGVLAGEPGAYRQARVIEALQI
                     PHTVQAILAARIDRLPDGAKRLLQSAAVVGTDVPLVLLQEIADEGDADLPGQLAALQA
                     GEFLYESRLFPDFEYTFRHVLTHDVAYGSLSQARRRALHARIAGAIERLRPKRLIEDV
                     DRLAHHTYRGEVWDRAVRYSQQAGAKAQQRSAHREAVAWLEQALAALGHLPAEADTRA
                     HAIDLRLELRGSLYSIGELEKMHGRLREAETLADQLGDARRRGWVSMHLGEYSRQIGH
                     FREASALIQRAREIAVAIDDMPLRLASDQYLGMAFHALGDYRRAAEHERRAAEFPRDE
                     PAPGGFGPTQAGSPAGFQVVSLGWLARCLAEIGQFDEGIEVGREAVRLAEEIDRPYTL
                     VSACWGLGYLHGVRGDLDAATLLLDRALTAARGAGLTRLLPQVMRALGSAYALMGRVA
                     DGTALLEGAVALSESINLRVAESNSLVLLGEVYLLGGQLDDGQVAGTRALAMARGRGQ
                     RGDEACALHLLGDVTAHREG"
     gene            complement(3202..4392)
                     /locus_tag="VNU03_09895"
     CDS             complement(3202..4392)
                     /locus_tag="VNU03_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HWP74818.1"
                     /translation="MPELSSRITALDALRGVGVLGILPVHMQSFAMVVAARVNPTVAG
                     DLQGPNGWIWLATAVLAEGKFISIFAMLFGAGLLLLSGRGGDGLFPVERLHRRRMALL
                     LGVGLLHAYLLWYGDMLVTFALCGAVVFGLRALPPRGLLTRGALALACASLIALGVGC
                     SLPWWSPDTKARFVQVWAPSREMVEREVAAYRGGWWGQMAYRVPEAFQIETSQLVLRG
                     FWETSGLMLVGMGLFRLGVLSGTRSARFYAAMTGLGFGVGLPIAWYGAARARALDWDL
                     ADFMVVGTQLAYWSDLLVGVGWVGLVMLLCRAGWRARAVAAVGRTALSNYLLQTVLCT
                     TIFYGHGLGLFARVERVGQLLIVLGIWIVQLAWSVWWTRRFAVGPLEWVWRRAARGSA
                     VPAR"
     gene            complement(4398..5399)
                     /locus_tag="VNU03_09900"
     CDS             complement(4398..5399)
                     /locus_tag="VNU03_09900"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP74819.1"
                     /translation="MKVATWRGGSRFSLDEAPDPTAGPGQVVVEVTAAGICGTDVHAT
                     QGLFPWTPPMVMGHEYSGVVREVGRGVNRRLLGRAVACEPSYGCGTCLPCKQGRISQC
                     ARATRVGGFAERAALPAHCVHPLPKGLDEVTAALAEPASCCLSGLEMFTMPRDATVVV
                     MGGGIMGLLTMVLARRRGAARLILSDPLEERRQLARKLGARVVVDPTRENLAERVMAL
                     TGGRGAEVVCEAVGKPELVAEAFAMVKPTGVLQLVGVNPKGSRMPIDLFDAHFREVRI
                     HGAYGRGTAFRRALALLPKLGVKRLVGAKFPLARIEEAFTHAAAGRGAKTVITPGVV"
     gene            complement(5396..6505)
                     /locus_tag="VNU03_09905"
     CDS             complement(5396..6505)
                     /locus_tag="VNU03_09905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP74820.1"
                     /translation="MTVKLGMFTMPFHHPDREYSAILEEDQEAIVLADRLGFTEAFVG
                     EHFSSWSERITSPLIFFATLIPRTTQIRFGTGVINLPQLHPATVAAHAAMFDQLCRGR
                     FIMGIGPGGLVSDLEMFDVGQAELRPQMVIESIETVLKLWAQDPPYEIDGRFWKISLT
                     NGIWPEFKVGYVPRPYQKPHPPIALSLLTPNSNSAATAGERGWIPVSGQFFHRRYLKG
                     HWEKYVEGCERAGRRPDPNIWRVSRSVLVTESDAEAEEYLADPDNGLSFYYHFFHHSF
                     SKGRKALFMLKPDLLIPDEDVTEDMIKRALVIAGSPRRVLEQLVALREETGHFGTLLM
                     GGHDWDRPALWRRSMELLATEVMPKFSQHASAPRT"
     gene            complement(6536..7135)
                     /locus_tag="VNU03_09910"
     CDS             complement(6536..7135)
                     /locus_tag="VNU03_09910"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWP74821.1"
                     /translation="MAQLTIPLDAKRFATGLTWKDYMAQMGDTRARTEQNYANSKLTD
                     EEQKFFAGATGVKHVMMLAENWCGDVHRNSPLIAHIVEAIPGAELRVFFRDQVPDLRD
                     TFLNNGYQSIPVVVFFDKDWNEIGRWMERAHGATGAATGIRARTLGAVPQGASKEQQD
                     AAMNEFRKQVQAEYDKPGGALWRAAAAEVKLLIEARAKK"
     gene            complement(7184..7465)
                     /locus_tag="VNU03_09915"
     CDS             complement(7184..7465)
                     /locus_tag="VNU03_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWP74822.1"
                     /translation="MLAAGASLGDLLEALGLDGTIDLTAAVDGELAERDTPLRDGADV
                     MLLVAMEGGAEAEGEATIRNAVKVVAVNQRVPGSECEAVAGSSSNRSKP"
BASE COUNT         1152 a         2834 c         2740 g         1064 t
ORIGIN      
        1 tcgccctcgc ggtgagccgt gacgtcgccg agcagatgga gggcgcaggc ttcatcgccg
       61 cgctgacccc gcccgcgcgc catcgcgagc gcgcgggtcc ccgccacctg gccgtcgtcg
      121 agctgtccac ccagcaggta cacctcgccg agcaggacga gcgagttcga ctcggcgacc
      181 ctcaggttga tggattccga gagggccacc gccccctcca gcagcgcggt gccgtcggcc
      241 acccggccca tcagcgcgta cgccgatccc agggcgcgca tcacctgcgg caggaggcgc
      301 gtcagcccgg cgccccgggc cgcggtcagc gcccgatcga ggagcagcgt ggcggcgtcg
      361 aggtcgcccc ggactccgtg gagatagccg agcccccagc acgcggacac gagcgtgtac
      421 gggcggtcga tctcctccgc gagacggacc gcctcgcgcc cgacctcgat cccctcgtcg
      481 aactggccga tctcggcgag gcagcgcgcg agccagccga gcgagactac ctggaatccc
      541 gccggcgagc cggcctgggt ggggccgaag cctcccggag cgggctcgtc ccgcggaaac
      601 tccgcggccc ggcgctcgtg ctccgcggcc cggcggtagt cgccgagggc gtggaaggcc
      661 atgccgaggt actggtcgga cgccagccgc aggggcatat cgtcgatcgc gacggcgatc
      721 tcgcgggccc gctggatcaa ggccgacgcc tcgcggaagt gcccgatctg acggctgtac
      781 tcacccagat gcatcgagac ccacccccgc cgccgcgcgt cgcccagctg atcggccagg
      841 gtctcggcct cgcggaggcg cccgtgcatc ttctcgagct cgccgatgga gtagagcgac
      901 ccgcgcagct cgaggcgcag gtcgatcgca tgcgcgcggg tgtcggcttc cgccggcagg
      961 tggccgagcg cggccagcgc ctgctcgagc cacgcgaccg cctcccgatg cgccgagcgc
     1021 tgctgggcct tggcgccggc ctgctgcgag taccggaccg cgcgatccca cacttctccc
     1081 cggtaggtgt ggtgcgcgag ccggtcgacg tcctcgatca gtcgcttcgg ccgcagccgc
     1141 tcgatcgccc ccgcgatgcg cgcgtgcagc gcgcgccggc gcgcctgcga gaggctgccg
     1201 taggcgacgt cgtgagtgag cacgtggcga aaggtgtact cgaagtcggg gaagagccgc
     1261 gactcgtaga ggaactcgcc ggcctgcagc gcggcgagct gccccggcag gtcggcgtca
     1321 ccctcgtccg cgatctcctg gagcaggacc agcgggacgt cggtgcccac caccgcggcc
     1381 gactggagga ggcgcttggc gccgtccggc agccggtcga tgcgggcggc caggatcgcc
     1441 tggacggtgt gcgggatctg gagggcctcg atcacccgcg cctgccggta ggcgccgggc
     1501 tctcccgcga gcacgccgac ctcgctcaag gtccggaccg tctcctcgag gaagaagggg
     1561 ttgcggtccg tcagctggac cagcagccgc ttgaggggcc ggagacccgg gtcccggccg
     1621 agcagggtct cgaggagctc gtcggtcgtc tccacgggca gagcctccac gcgaaactgc
     1681 gtgcagaccg agatgttggc ccagccgtgc tcgtattcgg gccggtaggt gaccaccagc
     1741 agcaggcgcg cggtgggcag gctcgccacc acctcgtcga ggagcgtctg ggtctcggtg
     1801 tcgatccagt gcaggtcctc gcacaggagc agcaccggcc gccgggcgct ctcccgcacg
     1861 agcagcatcc tcaccccatc gagcgcccgc cgccggcgct gcgtgggatc gagatccgcc
     1921 cagtgcggat cgtcgctcgg cacgtcgagg agccagagca gcgcggcgtg gtagcgggcc
     1981 agggccgggt cgagcgccat cagcttctcg gcgaccttct cccggacccg gggatgatcg
     2041 tcccggccct cgatcccgaa gtactgcttc aggagatcga tcaccggcga gtagctcgtg
     2101 ccgcggccgt acgacaccgc gccggtctcg agcacgaccc actcgcgtgc gtccggtgag
     2161 tgggtgaact cgtagcacag gcgggacttc ccgacgcccg cctccccgag gaccgcgacg
     2221 agctgcccgt gcccggcgcg ggcgcgcccc atcgccccga tcaggtgggc catctcggtg
     2281 gtgcgcccca cgaatcgcgt gaggccgcgc gtcgcggagg cgcgcaggcg ggaccgcgcg
     2341 ggcccggccc cggtcagctc gtaaacctcg accgggcccg cgagcccctt caccggcgtg
     2401 ggcccgagcg gcgtcacctg gatgaagcct tctgcgagcg cgagggtacc gggggtgagg
     2461 aggatgcgcc ccggatccgc gagctgctcc atgcgcgagg ccagatgggt ggtctggccc
     2521 accgcggtgt agtccatccg caagtcgctg cggatggtcc gcacgatcac ctcgcccgag
     2581 ttcaggccca cccggatcct caccgcgacg ccgagcgact gccggacctc ctccgcgtag
     2641 cgcttgatcg actcctgcat ctgcagcgcc gcgtagcagc cgcgcgtcgc gtgatcctcg
     2701 tgggcgagcg gcgcgccgaa cagcgccatg atcccgtcac ccatcacctg gttcacggtg
     2761 ccctcgtagc ggtgcaccgc ggccatcatg agctgcagga ccgggtcgag gacccggcgc
     2821 gcctcctccg ggtcgcggtc ggcgagcagc tccatcgagc tctggagatc ggcgaagagc
     2881 acggtcacca gcttccgctc gccctcgagg gccgccttcg agtcgaggat gcgggccgcg
     2941 aggtgcttcg gggtgtacga ctggggcggg ccgaagcgcg tccccccgcc gggagcggcg
     3001 ggagccagcg cggtgccgca gccgccgcag aatcgctcgg aggcctcgtt cgagaatccg
     3061 cacgcgaggc agcgcgacac gaacgagaga ccgcacgcgc cgcagaagcg tcggtccggg
     3121 cggttctcgg cgttacagcg cggacaattc accgaatcaa catacggtgg gccgcggggc
     3181 gggtcaacgg gcgaccgcgg gctagcgggc gggcaccgcg ctcccccggg ccgcccggcg
     3241 ccacacccac tcgagcgggc cgaccgcgaa gcgacgggtc caccagaccg accaggcgag
     3301 ctggacgatc cagatcccga gcacgatcag cagctggccg acccgctcca cgcgcgcgaa
     3361 gagcccgagg ccgtgcccgt agaagatcgt cgtgcagagg acggtctgga gcaggtagtt
     3421 gctaagcgcg gtccgaccca ccgcggcgac ggcgcgcgcc cgccatcccg cgcggcagag
     3481 gagcatcacc aggccgaccc atccgacgcc caccaataga tcgctccagt aggcgagctg
     3541 ggttcccacg accatgaagt cggccagatc ccagtcgagg gcccgggcgc gcgcggcgcc
     3601 gtaccacgcg atcggcagac ccaccccgaa cccgagcccg gtcatcgcgg cgtagaagcg
     3661 cgccgaccgg gtcccgctca gcacacccag cctgaacagg cccatcccca ccagcatcag
     3721 gccgctggtc tcccagaacc cccgcagcac gagctgcgac gtctcgatct ggaaggcctc
     3781 cgggacgcga tacgccatct gcccccacca tccgccccga tacgccgcga cctcccgctc
     3841 gaccatctcg cgcgacgggg cccagacctg aacgaaccgc gccttcgtgt ccggcgacca
     3901 ccagggcagc gagcatccca cgcccagcgc gatcagcgac gcgcacgcca gagccagcgc
     3961 gccgcgggtg agcaggccgc gcgggggcag cgcccgcagc ccgaacacca ccgcgccgca
     4021 gagcgcgaac gtgaccagca tgtcgccgta ccagaggaga taggcgtgca ggaggcccac
     4081 cccgaggagc agggccatgc gccggcggtg gaggcgctcg accgggaaga gcccgtcacc
     4141 cccgcggccc gagaggagca ggagcccggc tccgaacagc atcgcgaaga tcgagatgaa
     4201 cttgccctcg gccaggacgg ccgtggcgag ccagatccat ccgttcgggc cctggaggtc
     4261 gccggcgacg gtcgggttga cccgggccgc caccaccatc gcgaacgact gcatgtgcac
     4321 cggcaggatg cccagcacgc ccaccccgcg gagcgcgtcg agggcggtga tgcgcgacga
     4381 gagctccggc acccccgcta cacgacgccc ggcgtgatca cggtcttcgc cccccgcccg
     4441 gccgcggcgt gggtgaaggc ctcctcgatc cgcgcgagcg ggaacttcgc gccgacgagc
     4501 cgcttcactc cgagcttcgg cagcagcgcc agcgcgcggc ggaaggcggt gccgcggccg
     4561 taggcgccgt gaatgcggac ctcgcggaag tgcgcgtcga agaggtcgat cggcatccgg
     4621 ctgcccttgg ggttgacccc gaccagctgc aggacgccgg tcggcttcac catcgcgaag
     4681 gcctcggcga ccagctcggg cttgcccacc gcctcgcaca cgacctcggc gccgcggccc
     4741 ccggtcagcg ccatcacgcg ctcggccaga ttctcgcggg tcggatcgac caccacacgc
     4801 gcgcccagct tgcgcgcgag ctgccgccgc tcctcgagtg gatcggagag gatcaggcgc
     4861 gcggcgcccc ggcgtcgggc cagcaccatg gtgaggagcc ccatgatccc gccccccatg
     4921 accaccacgg tggcgtcgcg cggcatcgtg aacatctcga gtccggagag gcagcaggag
     4981 gcgggctccg cgagggcggc ggtgacctcg tcgagcccct tcggcagcgg gtgcacgcag
     5041 tgcgccggga gcgcggcgcg ctccgcgaag ccccccaccc gggtggccct cgcgcactgg
     5101 ctgatccgcc cctgcttgca gggcagacac gtgccgcagc cgtaggaggg ttcgcaggcg
     5161 accgcccggc cgagaagccg ccggttgacg ccgcggccga cctcgcgcac cacgcccgag
     5221 tactcgtggc ccatcaccat cggcggcgtc cacgggaaga ggccctgggt ggcgtgcacg
     5281 tcggtgccgc agatgccggc cgcggtgacc tcgaccacca cctgccccgg gccggcggtc
     5341 ggatccggcg cctcgtcgag gctgaaccgg ctgccgccgc gccaggtggc gaccttcacg
     5401 tccggggcgc gctcgcgtgc tgcgagaact tcggcatcac ctcggtggcg agcagctcca
     5461 tcgagcggcg ccagagcgcc ggccgatccc agtcgtggcc gcccatgagc agggtgccga
     5521 agtgacccgt ctcctcgcgg agggccacca gctgctcgag cacccggcgc gggctgcccg
     5581 cgatgacgag ggcccgcttg atcatgtcct cggtgacgtc ctcgtcgggg atcagcagat
     5641 cgggcttcag catgaagagc gccttgcggc ccttggagaa gctgtggtgg aagaagtggt
     5701 agtagaacga gagcccgttg tccgggtccg cgaggtactc ctccgcctcc gcgtcgctct
     5761 cggtgaccag cacgctgcgc gacacgcgcc agatgttcgg atcgggtcgc cgcccggccc
     5821 gctcgcagcc ctccacgtat ttctcccagt ggcccttgag atagcggcgg tggaagaact
     5881 ggccggatac cgggatccag ccccgctcgc ccgcggtggc cgcgctgttc gagttggggg
     5941 tgagcagcga gagcgcgatg ggcgggtgcg gcttctggta gggccgcggc acgtagccca
     6001 ccttgaactc gggccagatg ccgttggtga gcgagatctt ccagaagcgc ccgtcgatct
     6061 cgtagggcgg gtcctgggcc cacagcttga gcacggtctc gatggactcg atgaccatct
     6121 gcggccgcag ctcggcctgg cccacgtcga acatctcgag gtcgctcact aggccgccgg
     6181 ggccgatgcc catgatgaag cggccgcggc agagctggtc gaacatcgcg gcgtgggcgg
     6241 ccaccgtggc tggatgcagc tggggcaggt tgatgacccc ggtgccgaag cgaatctggg
     6301 tggtgcgcgg gatcagggtg gcgaagaaga tgaggggcga ggtgatgcgc tcgctccacg
     6361 acgagaaatg ctcgcccacg aaggcctcgg tgaagcccag ccggtccgcc aggacgatgg
     6421 cctcctggtc ctcttccagg atggccgagt actcgcggtc cggatggtgg aacggcatgg
     6481 tgaacatgcc gagcttgacc gtcatcgagg cctccgtggg cgcatggatc gcccgctact
     6541 tcttggcgcg ggcttcgatc aggagcttca cctccgccgc cgcggcgcgc cagagcgccc
     6601 cacccggctt gtcgtactcg gcctggacct gcttgcggaa ctcgttcatc gccgcgtcct
     6661 gctgctcctt ggacgcgccc tggggcaccg cgcccagcgt gcgggcccgg atgccggtgg
     6721 ccgcgccggt cgcgccgtgc gcgcgctcca tccagcggcc gatctcgttc cagtccttgt
     6781 cgaagaacac caccaccggg atggactggt acccgttgtt gaggaaggtg tcgcgcaggt
     6841 ccggtacctg atcgcggaag aagacccgca gctcggcacc gggtatcgcc tcgacgatgt
     6901 gggcgatcag cggcgagttg cggtgcacgt cgccgcacca gttctcggcg agcatcatca
     6961 cgtgcttgac cccggtcgcc cccgcgaaga acttctgctc ctcgtcggtg agcttggagt
     7021 tggcgtagtt ctgctcggtg cgggcccggg tgtcgcccat ctgggccatg tagtccttcc
     7081 aggtcaaccc ggtggcaaag cgcttcgcgt cgaggggaat cgtcagctga gccatgtcgt
     7141 cctcgcttcg gtgagagtga tgggtttggg acgttgccgg ctgctacggt ttactgcggt
     7201 tgctgctact gcctgctact gcctcgcact cactgcctgg cactctttgg tttactgcaa
     7261 ctactttcac tgcatttctg atggtcgcct cgccttcggc ttcggctccc ccttccattg
     7321 ccaccagcaa catgacgtcc gctccgtctc ggaggggggt atcgcgctcg gccagttcgc
     7381 cgtccaccgc cgcggtcagg tcgatcgttc cgtcgaggcc gagggcctcc agcaggtcgc
     7441 ccaggctcgc acccgccgcg agcacgtggc gctgggggcc gtccccgctg ccgggccgcc
     7501 ggcggcggag ggcggcggac agggtgaccg acacggcgac cgtcggggcc ccggtgcgcg
     7561 atgcgtcgtg aagccgactc atgaccacgg cggagtatac tacgccgcga tggggctcgg
     7621 ctcgccgcga tgaagcgccg caaccgcgac gggacggcgg aatggttcac gagcttcttc
     7681 gacgcggcct acgtggccca gctccgcgag gagaaacccc ccgcccagac ccgtcgcgag
     7741 gtcgacttcc tcctgcgcgc gctgcggccg gcgccggggg cccggatcct
//
LOCUS       DATMCV010000427         2660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_555366, whole genome shotgun sequence.
ACCESSION   DATMCV010000427 DATMCV010000000
VERSION     DATMCV010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555366"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VNU03_09920"
     CDS             <1..1050
                     /locus_tag="VNU03_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP74823.1"
                     /translation="FPEGDLSAVPRVPRLAVRVRLLAIFLLTAVVPLAVLGIVAYARG
                     LDLLDADPATARGIVSGLRTTVLFFVAVGVVVAVGLALFAANSVATPLKDIETAMARV
                     ERGHLDSHCPVVSTDEIGAVAEGFNRMLHGLREREQVKETFGKYVAPEIRDEILAGRI
                     SGDGELKEVTVLFADIRDFTPWVEATAPRDVVRDLNEYFTEMAEAIRAQRGLVLQFIG
                     DEIEAVFGAPIAARDHAAMAVRAALDMRARLHTWNAGREAAGKPALRHGIGIHTGTVL
                     AGNIGGAERLSYALVGDPVNLASRIQGLTKEFEVDILISEATRKSIDPAVPVEELPAV
                     RVKGRAEEVNVYKVV"
     gene            1063..2613
                     /locus_tag="VNU03_09925"
     CDS             1063..2613
                     /locus_tag="VNU03_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP74824.1"
                     /translation="MGSSRDSTSWVILRILGVGNLLGALVSFFYFRFIDSTAAELPEV
                     GLLEIGYSIVAFGVLVFIGYQVGRRWAAPLISWRGPEGLPDATVAMIRRRSLLFPYAL
                     AGISVVGWLLAGVLWGVVWPLLAGTLAPHRIARSIFGNTVIAGGVTCTFIFFSVEHLW
                     RRRIPKFFPDGDLSSVRNAPRFSVRARLLAIFFMVSLVPLSVLAMLAYTRALALVGAD
                     PAAAGEAVDGLRLIILFILAAGIVAAVGLSIFAANSVAAPLKDVETAMAEVERGRLDG
                     HAPVVSTDEIGAVAEGFNRMLHGLREREIVKETFGKYVTPEIRDEILAGRISGEGELK
                     EVTVLFADIRDFTPWVEATAPRDVVRDLNEYFTEMAEAIRAQHGLVLQFIGDEIEAVF
                     GAPIASRDHAAMAVRAALDMRGRLRAWNARREATGKPALRHGIGIHTGTVLAGNIGGA
                     ERLSYALVGDPVNLASRIQGLTKDFKVDILISEATRKSIDSGVTVEELPAVRVKGRAE
                     EVNVYKVV"
BASE COUNT          367 a          961 c          886 g          446 t
ORIGIN      
        1 ttcccggaag gtgacctgag cgcggtgccg cgcgtgcccc gcctcgcggt gcgcgtccgg
       61 ttgctcgcca tcttcctgct caccgcggtg gtcccgctcg cggtgctcgg aatcgtggcg
      121 tacgcgcggg gactcgacct gctcgacgcc gacccggcca ccgcccgggg aatcgtgtcc
      181 ggcctgcgca cgacggtgct gttcttcgtg gccgtcggtg tcgtcgtcgc ggtcggcctg
      241 gccctcttcg cggccaacag tgtcgcgacg cccctgaagg atatcgagac cgccatggcc
      301 cgggtcgagc ggggccacct cgacagccac tgcccggtgg tcagcaccga cgagatcggc
      361 gcggtggccg agggcttcaa ccgcatgctg cacggcttgc gcgagcgcga gcaggtcaag
      421 gagaccttcg gcaagtacgt cgcccccgag atccgtgacg agatcctcgc cggccgcatc
      481 agcggcgacg gagaattgaa ggaggtcacc gtcctcttcg ccgacatccg cgacttcacc
      541 ccctgggtcg aagccaccgc tccccgcgac gtcgtccgcg acctcaacga gtacttcacc
      601 gagatggccg aggccatccg cgctcagcgc ggcctggtgc tgcagttcat cggcgacgag
      661 atcgaggcgg tcttcggcgc ccccatcgcc gcccgcgacc acgccgccat ggccgtgcgc
      721 gccgcgctcg acatgcgcgc ccgcctccac acctggaacg ccggccgtga ggccgccggc
      781 aagcccgcgc tgcggcacgg catcggcatc cacaccggca ccgtgctggc cggcaatatc
      841 ggcggcgccg agcgcctctc gtacgccctg gtcggcgatc cggtcaacct cgcttcccgc
      901 atccagggcc tcacgaagga gttcgaggtc gacatcctca tcagcgaggc cacccgaaag
      961 agcatcgatc ccgcggtgcc ggtcgaggag ctccccgcgg tccgcgtcaa gggccgcgcc
     1021 gaggaagtca acgtgtacaa ggtggtgtga gaccttctcg tcatgggctc ttcccgagat
     1081 tcgacgtcgt gggtcatcct ccgcatcctg ggcgtcggca acttactcgg cgccctcgta
     1141 tcgttcttct atttccggtt catcgactcg actgcggcgg agctgccgga agtgggactt
     1201 ctcgagatcg gctactcgat tgtcgcgttc ggcgtgctcg tcttcatcgg gtaccaggtc
     1261 ggccggcgtt gggccgctcc cctgatcagc tggcgcgggc ccgaaggtct tccggacgcc
     1321 acggtcgcca tgatccgccg gcgctcgttg ctcttccctt atgcgctggc aggtatctcg
     1381 gtcgtgggct ggctgctggc gggcgtgctc tggggcgtcg tctggccgtt gctcgccggc
     1441 accctggcgc cgcaccgtat cgcccgctcg atcttcggca acacggtgat cgccggcggc
     1501 gtgacctgca cgttcatctt cttctcggtc gagcacctgt ggcggcgccg gatccccaag
     1561 ttcttccccg acggcgacct gagcagcgtc cggaatgccc cgcggttctc ggtccgcgcc
     1621 cgactgctcg cgatcttctt catggtgagc ctcgtccccc tgagcgtgct cgcgatgctg
     1681 gcgtacacgc gggcgctggc cctggtgggc gccgatcccg cggcggccgg cgaggccgtg
     1741 gacggactgc gcctgatcat cctcttcatc ctcgcggcgg gcattgtcgc ggcggtgggc
     1801 ctctccatct tcgcggccaa tagcgtcgcg gccccgctga aggacgtcga gaccgcgatg
     1861 gccgaggtcg agcggggccg cctcgacggc cacgccccgg tggtcagcac cgacgagatc
     1921 ggcgcggtgg ccgagggctt caaccgcatg ctccacggcc tgcgtgagcg cgagatcgtc
     1981 aaggagacct tcggcaagta cgtcactccc gagatccgcg acgagatcct cgccggccgc
     2041 atcagtggcg agggagagct gaaggaggtc accgtcctgt tcgcggacat ccgtgacttc
     2101 actccgtggg tcgaggccac cgctccccgc gatgtcgtcc gcgacctcaa cgagtacttc
     2161 accgagatgg cggaggccat ccgcgcccag cacggcctcg tgctgcagtt catcggcgac
     2221 gagatcgagg cggtgttcgg agccccgatc gcctcgcgcg atcacgccgc gatggcggtg
     2281 cgggccgcgc tcgacatgcg cgggcggctc cgcgcctgga atgcccgccg cgaggccacc
     2341 ggtaagcccg cgctgcggca cggcatcggc atccataccg gcaccgtcct ggccggcaac
     2401 atcggcggcg cggagcggct ctcctacgcg ctggtgggcg atccggtgaa cctcgcttcc
     2461 cgcatccagg ggctcaccaa ggacttcaag gtcgacatcc tcatcagcga ggccacccgc
     2521 aagagcatcg acagcggcgt gacggtcgag gaactgcccg cggtgcgcgt gaagggccgc
     2581 gccgaggaag tgaacgtcta caaggtggtc tgacggtggc gctccacccg ttccgcttcg
     2641 gcgtcaccgt cccgaccgtc
//
LOCUS       DATMCV010000428         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_91476, whole genome shotgun sequence.
ACCESSION   DATMCV010000428 DATMCV010000000
VERSION     DATMCV010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91476"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..688
                     /locus_tag="VNU03_09930"
     CDS             74..688
                     /locus_tag="VNU03_09930"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HWP74825.1"
                     /translation="MSNDPVRARSQFFGGRVCLDFANTVDWRTSASPQELIPDYATLL
                     AWSRMRGTLPAAAITRLRARSVKRAAAAAAVLDDAHALRIEIWQAAIALSGGRPAPLQ
                     PFNRRLAALPAQPPLVRSGARYVLALPGKALHEPLWPVLWSLTALLSSEDGGRVGSCR
                     AEGCGWFFVDESPNRSRMWCSSDVCGNRERARRAYDKRRSKPRG"
     gene            774..1157
                     /locus_tag="VNU03_09935"
     CDS             774..1157
                     /locus_tag="VNU03_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74826.1"
                     /translation="MWAPTRESFPVLAISGYLAFTAATMLAVVASDAQSPRAAVGLLA
                     VSLPALAAWLYIESTGWVGTELASRIVRRAVIAVALGFSLAGFLTVIWAFSRLAVFAI
                     VLEASVWYLAISGLSYLREEPDPRR"
     gene            complement(1436..>2381)
                     /locus_tag="VNU03_09940"
     CDS             complement(1436..>2381)
                     /locus_tag="VNU03_09940"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74827.1"
                     /translation="LAAPLAPEAQPAARIPTVGLLLPTTPASSAHLVEAFRQGLRELG
                     DVGGNTFTLLLRYAEGRSERLPELARDLVSLKVDVIVTSTDLAIAAVKRETRTIPTVM
                     AFSGDPVGTGFVASLARPGGNVTGLSGVSPELSGKRLELLKQVVPGLARVAFLWNPDV
                     RGAVLDYKETEATASSLHLELQSVELSAAADVGRAFSVLTGQRAQALIVPPGSAVMLS
                     NRAEILALTLKNRLPAMFPVKEWVIEGGLMSYGPNLPAMYRRAATYADKILKGAKPAD
                     LPVERPTKFELAINLKIAKVLGLTIPPSLLQRADQVIE"
BASE COUNT          367 a          826 c          794 g          394 t
ORIGIN      
        1 aggcggtgaa cggcggccgg gccgcgccgg gttctctcat ggtcaagggc atttagaagt
       61 cgagggacgc tcgatgtcaa atgatccggt ccgcgcccgc tcgcagttct tcggcggccg
      121 cgtctgcctg gacttcgcca acaccgtcga ctggcgcacc tccgcgtctc cccaggagct
      181 gattcccgac tacgccaccc tgcttgcctg gagtcgcatg cgcggcacgc tgcccgcggc
      241 cgcgatcacg cggctccggg cgcgcagcgt gaagcgggcg gccgccgcgg cggcggtgct
      301 cgacgacgcg cacgccctgc ggatcgagat ctggcaggcc gcgatcgcgc tcagcggcgg
      361 gagaccggcc ccgctgcagc cgttcaaccg gcggctcgcg gccctgcccg cccagccgcc
      421 cctggtcagg agcggggcgc gctacgtgct cgccctgccg ggcaaggcgc tgcacgagcc
      481 gctctggccc gtgctctggt cgctgaccgc gctgctctcg tccgaggacg gcgggcgcgt
      541 gggctcctgc cgggcggaag ggtgcgggtg gttcttcgtg gacgagagcc cgaaccgctc
      601 ccggatgtgg tgctcgagcg acgtctgcgg caatcgcgag cgcgcccgcc gcgcctacga
      661 caagcgccgg tccaagcccc gcgggtgagt tgcacccggg cgcgtggatc gcctatagtc
      721 cgagacgcat cacccggctg aggagaagca gacgggcctt cgtggaggtc cacatgtggg
      781 cgccgacgcg tgagtccttc cccgtcctcg cgatctctgg ctatctggcc ttcaccgcgg
      841 ccaccatgct cgcggtggtc gcttccgatg cccaatcgcc ccgcgcggcg gtcggtctcc
      901 tcgcggtctc gctgcccgcg ctggccgcct ggctgtacat cgaatccacc ggatgggtgg
      961 ggacggagct ggccagccgg atcgtgcgcc gggccgtcat cgcggtggca ctcgggttca
     1021 gcctcgcggg cttcctgacc gtgatctggg ccttctcacg gctcgcggtg ttcgcgatcg
     1081 tcctggaggc gtccgtgtgg tacctggcga tcagcgggct gtcctacctg cgggaggagc
     1141 cggacccgcg gcggtgaggc gcgcgcggcg atccgcgcga gcgagaaaag tggtaggcgg
     1201 ggggaggatt gaactcccga cctcggccga gcacttgcag accgacaacg gcgactgtct
     1261 ccaccccgcg gctttccatt gatggggcaa tggcccacaa gttgctcttc gacggcttcg
     1321 aattgggttc cttccctccg cagctcacgt gagatgctca ccaccccgaa gagaccaccc
     1381 tctttccaaa taaactcttc ccgatagccc atccagtact gacgttcatc cagcatcact
     1441 cgatcacctg atcggcccgc tgcaggagcg acggcgggat cgtcaggccg aggaccttgg
     1501 cgatcttgag attgatggcc agctcgaact tcgtcgggcg ttccacgggc agatcggcag
     1561 gcttggcgcc tttcaggatc ttgtccgcgt acgtggccgc ccgacggtac atggcgggca
     1621 ggtttggccc ataggacatg agcccgccct cgattaccca ctccttgacg ggaaacatgg
     1681 ccggaagccg attcttcagc gtcaaggcca ggatctccgc tcggttcgac aacatcacgg
     1741 cgctcccagg cggcacgatt aacgcctgcg cgcgttggcc agtcagaaca gagaaagcgc
     1801 ggccgacgtc cgccgcagcg gagagttcaa cggattggag ttcaagatgc agggaactcg
     1861 cggtcgcctc ggtctccttg tagtcgagaa cggcgccccg cacgtcggga ttccagagga
     1921 acgctacgcg agcgagaccc gggacgacct gcttcagcag ctccagccgt ttgccactca
     1981 gctccggcga gacaccgctg agcccggtca cattgccacc gggccgcgcc aggctcgcca
     2041 cgaacccagt cccgaccggg tctccactga aggccattac ggtcggaatc gtccgcgtct
     2101 ctcgcttgac cgccgcgatc gccagatcgg tgcttgtcac gatcacgtcg accttgaggc
     2161 tgaccaggtc ccgtgcaagc tcgggaaggc gctcggatct accttcggcg tagcgaagca
     2221 gcaaggtgaa ggtgttcccc ccgacatcgc caagctcccg caatccttgc ctgaacgcct
     2281 ccacgaggtg cgctgagcta gccggagtgg tcggaagaag cagcccgacc gtcggaatcc
     2341 gcgccgccgg ctgcgcctca ggggcgagcg gcgcggcgag g
//
LOCUS       DATMCV010000429         2654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_91665, whole genome shotgun sequence.
ACCESSION   DATMCV010000429 DATMCV010000000
VERSION     DATMCV010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91665"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..795
                     /locus_tag="VNU03_09945"
     CDS             73..795
                     /locus_tag="VNU03_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74828.1"
                     /translation="MRFGGHVAVDHVSLDVAPFTLKSIIGPNGAGKTTFFNLLSGQYR
                     PTAGRILFEGRDITRRGAAARTRLGIGRSFQLTNIFPTLSVLENVRLAVQARRGLGFT
                     AWRDYRRFSDLEDTAYGLLETVLLEGKWRAAAGTLAHGEKRKLELGILLALEPAVLLL
                     DEPTAGMSLEEVPAIIELIERIRARRDRTILLVEHKIDMVMALSDSIAVLQDGRLIAD
                     GPPAAVSASAEVQAAYFGTARA"
     gene            788..1492
                     /locus_tag="VNU03_09950"
     CDS             788..1492
                     /locus_tag="VNU03_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74829.1"
                     /translation="MPDVLELEGVHTDIGQHHILQGVSLRVRPDAVTVLLGRNGAGKT
                     TTMRTVMGVLPLKRGAVRFEGRAIQGLPPHAIARLGIGYVPEGQGIFATLTVEENLRV
                     AMRAETAECRERMRRVLALFPDLERFRPARAGTLSGGQKQMLAIGRALVNPTRLLLID
                     EPSKGLAPIVIEHLIAALEAMKRETTVVLVEQNFAMASRLGDDCYVMDDGRTVYAGSM
                     AALAADRNLKQKYLGV"
     gene            1489..2466
                     /locus_tag="VNU03_09955"
     CDS             1489..2466
                     /locus_tag="VNU03_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74830.1"
                     /translation="MRRVLPLAVGAALLLLLPLGAIEPRAWLTLTVAGLAMGMLIFLV
                     ASGLTLIFGLMDVLNFAHGALFSWGAYAGFTVAVAINGRWGWAASPSIWLNAGLLLLA
                     VAAAVAVAFTLGVALERVIVRPVYGQHLFQILITLGATIVLEELIRVAWGPNDQVMPV
                     PATFQGSWDVADVVVLRFPAIAIGLGLLVYGSMQWVLTRTRVGLIVRAGVENREMVQV
                     MGHDIHRYFTAVFAAGSGLAGLGGLMWGMFSQSIRPSMGGEQLIFAFIVVIIGGLGSV
                     TGSLVGALLVGLAYNYVAYLVPKAAIGVTMGIMVLVLLVRPTGLYGAGR"
     gene            2463..>2654
                     /locus_tag="VNU03_09960"
     CDS             2463..>2654
                     /locus_tag="VNU03_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP74831.1"
                     /translation="MSAGALTPVQAGRAAARRERWRAPTLYLRLAVVLAWVLLPTVGP
                     SFQVLDLAVKIALFGTLAAS"
BASE COUNT          325 a          948 c          961 g          420 t
ORIGIN      
        1 cgcgctgcgc gccttcctcg agggggccta gcgacggccg cggccccggt catctccacc
       61 cgcgacctca ccatccgctt cggcggtcac gtcgcggtcg accacgtcag cctcgacgtg
      121 gctccgttca cgctcaagtc catcatcggg cccaacgggg cgggcaagac caccttcttc
      181 aacctgctga gcggccagta ccggccgacc gcgggccgca tcctgttcga ggggcgcgac
      241 atcacccggc gcggggcggc ggcccgcacc cggctcggga tcggccgctc cttccagctc
      301 accaacatct tcccgacgct cagcgtgctc gagaacgtgc gcctcgcggt gcaggcgcgg
      361 cgcgggctcg gcttcaccgc ctggcgggac taccgccgct tctccgacct cgaggacacc
      421 gcgtacggcc tgctcgagac ggtgctgctc gagggcaagt ggcgcgcggc ggccgggacg
      481 ctggcccacg gcgagaagcg caagctcgag ctgggcatcc tgctcgcgct cgagccggcg
      541 gtgctgctgc tcgacgagcc gaccgcgggg atgtcgctcg aggaggtgcc cgcgatcatc
      601 gagctcatcg agcggatccg ggcgcgccgg gaccgcacca tcctgctcgt cgagcacaag
      661 atcgacatgg tcatggcgct gtcggactcc atcgcggtgc tgcaggacgg ccggctcatc
      721 gccgacggtc cgcccgccgc ggtcagcgcg agcgcggagg tgcaggccgc ctacttcggg
      781 accgcgcgtg cctgacgtgc tcgagctgga gggggtgcac accgacatcg ggcagcacca
      841 catcctccag ggcgtctccc tgcgggtgcg gccggacgcg gtcacggtgc tcctcgggcg
      901 gaacggggcc ggcaagacca ccaccatgcg caccgtgatg ggggtgctgc cgctgaagcg
      961 gggcgcggtg cgcttcgagg ggcgcgcgat tcagggcctg ccgccccacg cgatcgcgcg
     1021 gctcggcatc ggctacgtgc ctgaagggca ggggatcttc gccaccctca cggtggagga
     1081 gaatctgcgg gtggccatgc gggccgagac cgcggagtgc cgcgagcgga tgcgccgcgt
     1141 gctcgccctc tttcccgacc tcgagcgctt ccggcccgcg cgggccggca ccctctcggg
     1201 tggccagaag cagatgctcg ccatcgggcg ggcgctcgtc aacccgaccc gcctgctcct
     1261 gatcgacgag ccgtccaagg gccttgcgcc catcgtgatc gagcacctga tcgcggcgct
     1321 cgaggccatg aagcgggaga ccaccgtggt gctggtggag cagaacttcg cgatggcgag
     1381 ccggctcggc gacgactgct acgtgatgga cgacggccgc accgtctacg cgggatcgat
     1441 ggccgcgctg gccgccgatc gcaacctcaa gcagaagtac ctcggggtat gaggcgcgtc
     1501 cttcccctcg cggtgggcgc ggcactgctc ctgctgctgc cgctcggggc catcgagccg
     1561 cgggcgtggc tcaccctcac ggtggccggg ctcgcgatgg gcatgctgat cttcctggtg
     1621 gcctcggggc tcaccctgat cttcggcctc atggacgtgc tcaacttcgc ccacggcgcg
     1681 ctcttctcct ggggcgccta cgccggcttc acggtggcgg tggcgatcaa cgggcgctgg
     1741 ggctgggccg cctccccgtc gatctggctg aacgcgggcc tgctcctgct cgcggtggcc
     1801 gccgcggtgg cggtcgcctt caccctcggc gtcgcgctgg agcgggtgat cgtgcgaccg
     1861 gtctacgggc agcacctctt ccagatcctg atcacgctcg gggccaccat cgtgctcgag
     1921 gagctgatcc gcgtcgcgtg gggaccgaac gaccaggtga tgccggtgcc cgccaccttc
     1981 cagggctcgt gggacgtggc cgacgtggtg gtgctccgct tccccgcgat cgcgatcggg
     2041 ctcggcctgc tcgtctacgg ctccatgcag tgggtgctca cccgcacgcg ggtggggctg
     2101 atcgtgcgcg ccggcgtcga gaaccgggag atggtgcagg tgatgggcca cgacatccac
     2161 cgctacttca ccgcggtgtt cgcggccggc tccgggctcg cggggctggg cgggctcatg
     2221 tggggcatgt tcagccagtc gatccggccc tcgatgggcg gcgagcagct catcttcgcg
     2281 ttcatcgtgg tgatcatcgg cggtctcggc agcgtcaccg gctcgctggt cggcgcgctg
     2341 ctggtgggcc tcgcctacaa ctacgtggcg tacctggtgc ccaaggccgc gatcggggtg
     2401 accatgggca tcatggtgct ggtgctgctc gtgcgcccga ccgggctcta cggagccggc
     2461 aggtgagcgc gggtgccctc accccggtgc aggccggccg cgcggcggcg cgccgcgagc
     2521 gctggcgggc gcccaccctc tacctgcgcc tggcggtggt cctggcgtgg gtgctgctcc
     2581 cgaccgtggg gccgtccttc caggtgctcg acctggcggt gaagatcgcg ctcttcggca
     2641 ccctcgcggc gagc
//
LOCUS       DATMCV010000430         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_222513, whole genome shotgun sequence.
ACCESSION   DATMCV010000430 DATMCV010000000
VERSION     DATMCV010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222513"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1880)
                     /locus_tag="VNU03_09965"
     CDS             complement(<1..1880)
                     /locus_tag="VNU03_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HWP74832.1"
                     /translation="MSAREPGAEDRMEPIAIVGMAGRMPGAENLQQYWSNLRDGVESI
                     AFFSDEELAAAGVDPALLRNPRYVRARGVLQGAELFDAGLFGLSPREAEIMDPQHRVF
                     LETAWEALENAGYAPGVAALAAGVYAGASLNSYLIASLMTNPEVMLAAGAYQAMLAND
                     KDFLATRVSYKLNLKGPGVTIQTACSTSLVAVQMACQALLTRQCDLALAGGVSINVPR
                     VTGYLYEPGMILSPDGHCRAFDARAQGTVAGEGAGVVVLKRLRDALADGDHVRAVIRG
                     AAINNDGSLKVGYTAPSVDGQAAVIARAHAMAGFDPSTITYVEAHGTGTELGDPIEIA
                     ALTKAFRAGTDRRGFCALGSVKTNIGHLDAAAGVAGLIKTVLALEHRALPPSLHFEAP
                     NPNIDFGSGPFRVNAGLADWARSDDAPRRAGVSSFGIGGTNAHVVLEEAPALPAPAPS
                     RPVQIFTLSARSDEALERAGAAFAEHLVAEHSELAPGDVAFTLHRGRATFPHRRVVVA
                     ASLDEAARALREGDARRVLSRVEAGKDRPVVFMFSGQGSQYVGMGRGVYESEPLFREI
                     VDRGAEALAPSLGLDLRTVLYPAPEAADAARARLATTAITQPALFVVEYALARLYMEW
                     GLR"
     gene            complement(1877..>2284)
                     /locus_tag="VNU03_09970"
     CDS             complement(1877..>2284)
                     /locus_tag="VNU03_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HWP74833.1"
                     /translation="ELRAFLKEYLPDYMVPSTFTSLPSLPLTPNGKVDRKRLPEPEDR
                     RAVSQAPFLAPRGRTEDALAAVWREVLRLEQVGIDDNFFDLGGHSLLLVQVQAKLRSG
                     LGQDVPIVEMFQYPTIRTMAAHLSRAAAHAGVS"
BASE COUNT          332 a          840 c          808 g          304 t
ORIGIN      
        1 cgcaggcccc actccatgta caggcgggcc agcgcgtact ccacaacgaa cagcgcgggc
       61 tgggtgatcg cggtcgtcgc gagccgggcg cgagctgcgt cggcggcctc gggcgcgggg
      121 tagagcaccg tgcgcaggtc gaggccgagg ctcggcgcga gcgcctccgc gccgcgatcg
      181 acgatctccc ggaagagcgg ctccgactcg tacaccccgc gccccatgcc cacgtactgc
      241 gagccctggc ccgagaacat gaagaccacc ggccggtcct tcccggcctc gacccggctc
      301 agcacgcggc gcgcatcgcc ctcgcggagg gcccgggccg cctcatccag gctcgccgcc
      361 accaccacac ggcggtgcgg gaacgtggcc cggccgcggt gtagggtgaa ggccacgtcg
      421 ccgggggcca actcggagtg ctccgcgacg aggtgctccg caaaggcggc gccggcgcgc
      481 tccagcgcct cgtcggagcg ggcggacagg gtgaagatct gcaccggacg cgagggagcg
      541 ggcgccggga gcgcgggcgc ttcctccagc accacgtgcg cgttggtgcc tccgatgccg
      601 aacgagctca cgccggcgcg gcgcggggca tcgtccgacc gcgcccagtc ggcgagaccc
      661 gcgttcaccc ggaacgggcc gctcccgaag tcgatgttcg ggttcggcgc ctcgaagtgc
      721 aggctcggcg gcagggcgcg gtgctcgagg gccaggaccg tcttgatgag ccccgcgacg
      781 cccgcggcgg cgtcgagatg gccgatgttg gtcttcaccg aaccgagcgc gcagaacccg
      841 cgccggtcgg tgccggcccg gaaggccttg gtcagcgccg cgatctcgat gggatcgccg
      901 agctcggtgc cggttccgtg tgcctccacg taggtgatcg tggagggatc gaagcccgcc
      961 atcgcgtgag cccgggcgat gaccgcggcc tgcccgtcca cgctcggggc ggtgtagccc
     1021 accttcaagg agccatcgtt gttgatggcc gcccctcgga tcaccgcccg tacgtggtcg
     1081 ccgtcggcga gcgcgtcgcg gaggcgtttg agcacgacca cccccgcgcc ctcgcccgcc
     1141 accgtgccct gcgcgcgcgc gtcgaacgcg cggcagtggc catcggggga caggatcata
     1201 cccggctcgt agagatagcc ggtgacccgc ggcacgttga tcgagacgcc gcccgcgagc
     1261 gccaggtcgc actggcgggt gagcagagcc tgacaggcca tctgcaccgc gaccagcgag
     1321 gtggagcaag cggtctggat ggtgacgccc gggcccttca ggttcagctt gtaggacacc
     1381 cgcgtggcga ggaagtcctt gtcgttggcc agcatcgcct ggtaggcccc ggcggccagc
     1441 atcacctccg ggttcgtcat caggctcgcg atcaggtaac tgttcaggct cgcccccgcg
     1501 tacaccccgg cggcgagcgc agcgacaccg ggcgcgtagc ccgcgttctc gagcgcctcc
     1561 cacgcggtct cgaggaacac gcggtgctgg gggtccatga tctcggcctc acgcgggctc
     1621 agtccgaaca agcccgcgtc gaacagctcg gcgccctgca gcacgccccg cgcgcgcacg
     1681 taccgcgggt tgcgcaggag cgccgggtcg acccccgccg ccgcgagctc ctcgtcgctg
     1741 aaaaacgcga tcgactccac cccgtcacgc aggttgctcc agtactgctg caggttctcc
     1801 gcgcccggca tgcggccggc catccccacg atggcgatcg gctccatccg gtcctccgcg
     1861 ccgggctcgc gcgcgctcat gagacacccg cgtgggccgc cgcccgcgag aggtgcgcgg
     1921 ccatcgtgcg aatcgtcggg tactggaaca tctccacgat cggcacatcc tgaccgagcc
     1981 cggagcgtag cttcgcctgc acctgcacca gcaggagcga gtggccgccg agatcgaaga
     2041 agttgtcgtc gatgccgacc tgctcgagcc ggagcacctc ccgccacacg gccgcgagcg
     2101 cgtcctcggt ccgcccccgg ggagccagga agggcgcctg cgacaccgcg cgacggtcct
     2161 ccggctccgg gagacgcttg cgatccacct tgccgttcgg ggtcagcggc aggctcggca
     2221 ggctcgtgaa ggtcgagggc accatgtagt cgggaaggta ctccttcagg aacgcgcgca
     2281 gctc
//
LOCUS       DATMCV010000431         5363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_555551, whole genome shotgun sequence.
ACCESSION   DATMCV010000431 DATMCV010000000
VERSION     DATMCV010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555551"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VNU03_09975"
     CDS             complement(<1..430)
                     /locus_tag="VNU03_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74834.1"
                     /translation="MKVSVLCFDVSDNAVGRAWLLARLLEPLGPVEIVGPRFGAAVWE
                     PLVEESIPVRSLPGGRLPAFATRLPALARMADGDLLYASKPRLTSAGIGYLARVAGHR
                     PLLLDVDDWEVGFFLRGGPWGTVGRALNLSNPSGLTWTWLM"
     gene            complement(427..1569)
                     /locus_tag="VNU03_09980"
     CDS             complement(427..1569)
                     /locus_tag="VNU03_09980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP74835.1"
                     /translation="MTILQIVSCRGWSSDAYWAARATVELERAGHDVTLVCKRRSEAR
                     VIRRARDAGVNHIETLTLTSGIGPVADVRDLRRLVAWLPEAQVLHVHRGKEHWLAALA
                     NRMSYLRRPLIRTRHIVQPVRPHALNRWLYGHATDQVVTVTEAIRRQLVAGGLGVERR
                     VVTMPGGVDVDQFRPGAGAPPDHGIRATLGLPHDVPIIGLVSGFRVMKGHQTVVDAAA
                     RLAAGGRRFHLLLIGQGPFVPKVRAQVEAAGLGDRISILGFVDDLPAAMAVLDIALYS
                     ALESDGMSRVLFEYLAAGVPVVASRVGVVPEILEDGRSALLVPSGEPGPLADAIARLL
                     DDPGRRREIGAAGAALVRARYSGARLAEQLTALYLSLAVASVQPSS"
     gene            complement(1566..2693)
                     /locus_tag="VNU03_09985"
     CDS             complement(1566..2693)
                     /locus_tag="VNU03_09985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP74836.1"
                     /translation="MSAERPLTVLHLVANRWWTGSAEPVIRLLTGLRARGHRVLLGLI
                     TGDRFEQKAREAGLEPLPGLSLEVRGNPVHAVGDLRRVRQAVRAERVDVVHVHHSHDH
                     WLGWLGRSPAGLVRTFHNARAVRLTGLSPWLYRHTDALIAVSREIEERGRRAGIPPER
                     LVRVDGVVDVGRFEDGDGAAIRKEFDLGSAPVVGCVARLAPRRGHELLIRAFQQLLPE
                     YPHARLLLVGKGEARARLETLVSDLGLAREVLFAGYRDADLPAVLQALDAFALMGAGS
                     DESCRAALEAMAAGRPVVGRRVGALPETVVHGVTGLLVDDDRPESVAAALRVLVARPE
                     QAAAMGRAGRERARTLFGSDAHAAQVERVYRRIVESREPDR"
     gene            complement(2690..3769)
                     /locus_tag="VNU03_09990"
     CDS             complement(2690..3769)
                     /locus_tag="VNU03_09990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP74837.1"
                     /translation="MKTVLHTEASPGLGGQEVRTLNEARWTADRGWRVLLACQPDGRL
                     LERAREAGVETAAIRMRGPLDPLGLVALARLIRRERVDLVHTHSSIDAWLGGMAARLC
                     RVPVVRTRHVSIAIRRGLNPVYRWLADRVVTSGEAIRQVVVGAGAPPERVVAIPAGVN
                     LEAFPFGGRAPDTARALDLGSPVIGSVAMFRGSKGHPQLLQAFAQLREKRPSASLLLV
                     GDGIRRAWVQQLAQAAGLAESVVFTGFRPDVPALLATMDCFVLASTRTEGVPQALLQA
                     FATGVPVVASRIGGIPEVVTDGETGLLVESDSVPALVAAIGRVLDDPDASARRARAAR
                     ALVEAHFSHARSIDRLLHLYEELLA"
     gene            complement(3766..4785)
                     /gene="waaF"
                     /locus_tag="VNU03_09995"
     CDS             complement(3766..4785)
                     /gene="waaF"
                     /locus_tag="VNU03_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HWP74838.1"
                     /translation="MSALDLDPRTGHIVVRTPNWLGDTVMALPMLAALRRAAGEARIT
                     LIGRWAPLLAGQGVGDVLLPYPRPLAARRQLARALAADRADVALLLPSSLEAALAATR
                     WRARRRVGYATDGRRALLTDAIPLPAPRLHQVDEYAALLSLLGLDGASPAPEWKLPAC
                     PTADAEVAGLLAGAELDAGARLVGLHLGAAFGSSKLWPPEAFGRLARELAADGLCPVL
                     LGTAEDAATAGEVARAAATPLASLVGRDRPGLLARLLARLRCLVSGDTGVAHLAAAVG
                     VATVTLFGPTDRRLTAPRGRGARALERPVPCAPCFLPTCPIDHICLTRIDPETVLREV
                     RLAVA"
     gene            complement(4782..>5363)
                     /locus_tag="VNU03_10000"
     CDS             complement(4782..>5363)
                     /locus_tag="VNU03_10000"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWP74839.1"
                     /translation="RAPWGNARVFPRGMLREPLSGLARAHVVVVTNPSGPDTVAEVTA
                     TVRRHNAAAAVLAARYQLQDAIETPGARRIPVAELAGRRLLAFAGLGSPQGFADTLDA
                     AGIRRVGFAEFPDHHWFTAGDLAELTRDARAAGAQGLVTTEKDWVRVRDLPPPPLPLW
                     VLPVQLVIESGAETWQRLLAAVLAPAALGRPCS"
BASE COUNT          730 a         2023 c         2063 g          547 t
ORIGIN      
        1 ccatcagcca ggtccatgta agaccgctcg gatttgagag attcagggcc ctcccaacgg
       61 tcccccacgg tccgccgcgc aggaagaagc ccacctccca gtcgtcgacg tcgaggagca
      121 gcggccggtg cccggccacg cgcgcgaggt agccgatgcc ggcgctcgtg agccgcggct
      181 tggaggcgta gaggaggtcg ccgtcggcca tccgggcgag tgcgggcagc cgggtcgcga
      241 aggccgggag ccggccgccg ggcagcgagc gcaccgggat cgactcctcc acgagcggct
      301 cccagaccgc ggcgccgaag cgcgggccga cgatctccac cggcccgagg ggctcgagca
      361 gccgggccag gagccaagcg cgcccgaccg cgttgtccga gacgtcgaag cagaggaccg
      421 agaccttcac gacgacggct ggaccgacgc gaccgcgagc gacaggtaga gggcggtgag
      481 ctgctccgcg agccgcgcgc cggagtatcg ggcgcggacc agcgccgccc cggccgcgcc
      541 gatctcgcgc cgccggcccg ggtcgtcgag cagtcgggcg atcgcgtcgg cgaggggccc
      601 gggctcgccg gacgggacca gcagcgcgct gcgcccgtcc tccaggatct cggggacgac
      661 gccgacgcga ctggccacca ccggcacacc ggcggcgaga tactcgaaga gcacgcgcga
      721 catcccgtcc gactccagcg cggagtagag agcgatgtcg agcaccgcca tcgcggcggg
      781 caggtcgtcc acgaagccca ggatcgagat gcggtcgccg agcccggccg cctcgacctg
      841 ggcgcgcacc ttcgggacga agggcccctg cccgatcagc agcaggtgga agcggcgacc
      901 gccggcggcg aggcgggccg ccgcgtcgac caccgtctgg tggcccttca tcacccggaa
      961 gcccgacacg aggccgatga tcggcacgtc gtgcggcagc ccgagcgtgg cccggatgcc
     1021 gtgatcgggc ggcgcccccg cgccgggccg gaactggtcc acgtccacgc cccccggcat
     1081 cgtcaccacg cggcgctcca ccccgaggcc tccggccacg agctggcggc ggatggcctc
     1141 ggtcacggtg acgacctggt cggtggcgtg gccgtagagc cagcgattga gggcgtgggg
     1201 ccgcaccggc tggacgatgt gccgggtgcg gatgaggggc cgacggaggt aagacatgcg
     1261 attcgcgagc gcggccagcc agtgctcctt gccccggtgg acgtggagga cctgggcctc
     1321 gggcagccac gcgacgagcc ggcgcaggtc gcgcacgtcg gccaccgggc cgatgccgct
     1381 cgtcagggtc agggtctcga tgtggttcac gcccgcgtcc cgcgcgcggc ggatgacgcg
     1441 cgcctcgctg cgccgcttgc agacgagcgt gacgtcgtgg cccgcgcgct cgagctcgac
     1501 ggtggcgcgc gcggcccagt aggcgtcgga ggaccagccg cggcacgaga cgatctggag
     1561 gatcgtcacc ggtccggctc gcgcgactcc acgatgcgcc ggtacacccg ctcgacctgg
     1621 gccgcgtggg cgtcggagcc gaagagcgtc cgggcccgtt cccgcccggc gcgtcccatc
     1681 gccgcggcct gctccggccg cgccacgagg acgcgcaggg ccgccgcgac cgactccggg
     1741 cgatcgtcgt cgaccagcag gccggtgaca ccgtgcacca ccgtctcggg cagggcgccg
     1801 acgcgccggc ccaccaccgg ccgcccggcg gccatggctt ccagcgccgc gcggcaggac
     1861 tcgtccgagc ccgcgcccat cagcgcgaag gcgtcgaggg cctgcagcac cgcggggagg
     1921 tccgcgtccc ggtagcccgc gaacaggacc tcgcgcgcga ggccgaggtc gctcacgagc
     1981 gtctccaggc gggcgcgcgc ctcgcccttg ccgacgagca gcagccgcgc gtgcgggtac
     2041 tcgggcagca gctgctggaa ggcgcggatc aggagctcgt gcccgcggcg gggcgccagg
     2101 cgcgccacgc agccgacgac cggcgcgctt cccaggtcga actccttgcg gatcgcggcg
     2161 ccgtcgccgt cctcgaagcg cccgacgtcc accaccccgt cgacccgcac cagccgctcg
     2221 ggcgggatcc cggcgcggcg gccgcgctcc tcgatctcgc ggctcaccgc gatgagagcg
     2281 tcggtgtgcc ggtagagcca gggggagagc ccggtgagcc gcaccgcgcg cgcattgtgg
     2341 aaggtgcgca ccaggccggc cgggctacgg ccgagccacc cgagccagtg atcgtgcgag
     2401 tggtggacgt ggaccacgtc gacgcgctcg gcccgcaccg cctgtcgcac ccggcggagg
     2461 tcgccgaccg cgtggaccgg attgccgcgc acctcgagcg agaggcccgg caggggctcg
     2521 aggcccgcct cgcgcgcctt ctgctcgaac cggtcgcccg tgatgaggcc gagcagcacc
     2581 cggtgcccgc gcgcgcggag gccggtcagc aggcggatca ccggctccgc gctgccggtc
     2641 caccagcggt tggccaccag gtgcagcacg gtcaggggcc gctccgcgct cacgcgagca
     2701 gctcctcgta gagatggagg agccggtcga tcgagcgcgc gtgggagaag tgcgcctcga
     2761 cgagcgcgcg ggccgcccgc gcccggcgcg cggacgcgtc ggggtcgtcg agcacgcgcc
     2821 cgattgcggc caccagcgcc gggaccgagt cgctctccac gaggaggccg gtctccccgt
     2881 cggtgaccac ctccgggatg ccgccgatgc ggctcgccac caccgggacg ccggtcgcga
     2941 aggcctggag cagggcctgc gggacgccct cggtgcgcgt cgaggcgagc acgaagcagt
     3001 ccatggtggc gaggagcgcg gggacgtcgg ggcggaagcc ggtgaagacc accgactccg
     3061 cgaggcccgc ggcctgggcg agctgctgga cccacgcgcg gcggatgccg tcgccgacca
     3121 gcaggagcga ggcggagggc cgcttctcgc gcagctgcgc gaaggcctgc aggagctggg
     3181 ggtggccctt ggagccccgg aacatcgcca cggagccgat caccggcgag cccaggtcga
     3241 gcgcgcgcgc ggtgtcggga gcccggccgc cgaacgggaa cgcctcgagg ttcaccccgg
     3301 ccgggatcgc gaccacgcgc tcgggcggcg cccccgcccc caccaccacc tggcggatcg
     3361 cttccccgct cgtgacgacc cggtcggcga gccagcgata gaccgggttg aggccccggc
     3421 ggatcgcgat cgagacgtgg cgcgtgcgca ccaccggcac gcggcagagc cgcgccgcca
     3481 tcccgccgag ccaggcgtcg atcgagctgt gggtgtgcac gagatcgacg cgctcgcgcc
     3541 ggatgagacg ggcgagcgcg acgagcccca gcgggtcgag cggcccccgc atgcggatgg
     3601 ccgccgtctc gactcccgcc tcccgggcgc gctcgagcag gcgtccgtcc ggctggcagg
     3661 ccagcaggac gcgccagccg cggtcggcgg tccagcgcgc ctcgttgagc gtgcgcacct
     3721 cctggccgcc gagtcccggc gaggcttcgg tgtggaggac ggtcttcacg cgaccgcgag
     3781 cctcacctcg cgcaggacgg tctccggatc gatccgcgtg aggcagatgt gatcgatcgg
     3841 acacgtgggc aggaagcacg gcgcgcacgg gaccggccgc tcgagggccc gggccccgcg
     3901 cccgcgcggc gcggtcaggc gccggtcggt ggggccgaac agggtcacgg tggccacccc
     3961 gaccgcagcg gccaggtgcg cgacaccggt gtctccgctc accaggcaac gcaggcgcgc
     4021 gagcaggcgc gccagcagcc cgggccggtc gcggcccacc agcgaggcca gcggcgtcgc
     4081 ggcggcgcgc gccacctccc ccgcggtggc ggcgtcctcc gcggtgccca ggagcacggg
     4141 gcacaggcca tccgcggcca gctcccgcgc gaggcggccg aaggcctccg gaggccagag
     4201 cttcgagctg ccgaacgccg cgccgagatg caggcccacg agccgtgcgc ccgcgtcgag
     4261 ctcggccccg gccaggagtc ccgcgacctc ggcgtcggcg gtcgggcacg cggggagctt
     4321 ccactcgggc gccgggctcg cgccgtccag gcccagcagc gagagcaagg cggcgtactc
     4381 gtccacctgg tgcaggcgcg gcgccggcag cgggatggcg tcggtcagca gcgcgcggcg
     4441 tccgtcggtg gcgtagccca cccgacgccg ggcgcgccag cgggtggccg cgagcgcggc
     4501 ctccagcgag ctcgggagca ggagcgccac gtcggcgcga tccgcggcca gggcccgggc
     4561 gagctgtcga cgcgccgcga gcgggcgggg atacggcagc agcacgtcgc cgactccctg
     4621 cccggccagc agcggagccc agcgcccgat caaggtgatg cgcgcctctc ccgcggcccg
     4681 gcgcagggcg gcgagcatgg ggagcgccat cacggtgtcc ccgagccagt tcggggtccg
     4741 gaccacgatg tggcccgtgc gcgggtcgag atcgagcgcg ctcacgagca cggcctcccc
     4801 agcgcggccg gcgccaggac ggcggcgagc aggcgctgcc acgtctccgc cccggactcg
     4861 atcacgagct ggaccggcag cacccatagc ggcaggggcg gtggcggcag gtcgcgcacg
     4921 cgcacccagt ccttctcggt ggtgaccagt ccctgggccc cggccgcgcg cgcgtcgcgg
     4981 gtcagctcgg cgaggtcgcc cgcggtgaac cagtgatgat ccgggaactc cgcgaagccc
     5041 acccggcgga tccccgccgc gtcgagcgtg tcggcgaatc cttggggcga gcccagaccc
     5101 gcgaaggcca gcaggcggcg gcccgccagc tcggccaccg ggatccgccg ggctcccggc
     5161 gtctcgatcg cgtcctggag ctggtagcgc gcggccagca ccgccgcggc cgcgttgtgg
     5221 cggcgcaccg tggcggtcac ctcggccacc gtgtccgggc ccgacggatt ggtcaccacc
     5281 accacgtggg cccgggccag tccggagagc ggctcgcgca gcatgccgcg ggggaagacg
     5341 cgggcgttgc cccacggcgc gcg
//
LOCUS       DATMCV010000432         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_490248, whole genome shotgun sequence.
ACCESSION   DATMCV010000432 DATMCV010000000
VERSION     DATMCV010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490248"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VNU03_10005"
     CDS             complement(<1..196)
                     /locus_tag="VNU03_10005"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HWP74840.1"
                     /translation="MSGFRFELVKTDGAARLGRILTAHGAVETPAFMPVATQGSVKSL
                     APSDLLAAGAEIVLANTYHLM"
     gene            complement(200..1237)
                     /gene="queA"
                     /locus_tag="VNU03_10010"
     CDS             complement(200..1237)
                     /gene="queA"
                     /locus_tag="VNU03_10010"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWP74841.1"
                     /translation="MDTSLFDYELPPELIAQEPAEPRDASRLMVLDRAAGGWEDRRFS
                     ELPELLRAGDCLVANRSRVMPARLLGVAEPGGQAVELLLIRPVGDERWEAMVRPGRRF
                     RPGARIGLAGGAALAQVVGEGSEGTRVVAIEAPWPVRELMERHGLPPLPPYIGRHDAP
                     KPEDRERYQTVYAREDGSVAAPTAGLHFTPELLARLERCGVDVHYLTLHVGPGTFRPL
                     RAGRVEEHRMEAEPIDIPESTAAAVERARREGRRVVAVGTTTTRALEWAAGEDGRVRA
                     GTGPADLFIRPGHRFRVVDALVTNFHLPRSSLLVLVSAFAGRELVLAAYRHAVAARYR
                     FYSYGDAMLIH"
     gene            complement(1513..1869)
                     /locus_tag="VNU03_10015"
     CDS             complement(1513..1869)
                     /locus_tag="VNU03_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010392819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HWP74842.1"
                     /translation="MSAEPRPGGALAWEEFERVDMRVGVVIDAQEFPEARRPALKLAI
                     DFGPLGVKRSSAQITDRYTPAALVGRRVVAVVNFPPKRIGPFVSEVLVLGAYGEGGEV
                     ILLRPDFEVLPGARIG"
     gene            complement(1859..>2481)
                     /locus_tag="VNU03_10020"
     CDS             complement(1859..>2481)
                     /locus_tag="VNU03_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HWP74843.1"
                     /translation="VGRVPADSVVWTAVRETAGQVLLWQGELFPAFYHTDSGGYTEDP
                     RVVFAASNMPALKPVRVEFPSESPHQRWRLDLPLSELSAALLRGGISIGRVVALDVLE
                     RSVSLRVARIAVRGTAGTVTLRGNDLRRLVGYDTLKSTLFAVAVVGPMVRFVGRGYGH
                     GVGLDQWSAKTMADQGYNAQRIVLYYYPGATLSTLPVLSGAPPIVR"
BASE COUNT          383 a          961 c          845 g          292 t
ORIGIN      
        1 gcatcaggtg gtaggtattg gccagcacga tctcggcgcc cgcggcgagc agatccgagg
       61 gagcgaggct cttgaccgag ccctgcgtcg ccaccggcat gaaggccggc gtctcgaccg
      121 ccccgtgggc ggtgaggatc cggccgaggc gcgcggcgcc gtcggtcttg acgagctcga
      181 agcggaagcc gctcatgcct cagtggatca acatcgcgtc gccgtacgag tagaagcgat
      241 agcgggccgc caccgcgtgg cggtaggcgg ccaggacgag ctcgcggccc gcgaaggccg
      301 acaccaggac gagcaggctc gagcgcggca ggtggaaatt cgtgacgagc gcgtccacca
      361 cgcggaagcg gtggccgggc cggatgaaca gatccgccgg ccccgtcccc gcgcgcaccc
      421 ggccgtcctc gccggccgcc cactcgagcg cccgggtggt cgtcgtcccc accgcgacca
      481 cgcggcgccc ctcgcgccgg gcccgctcga ccgccgccgc ggtcgactcg ggaatgtcga
      541 tgggctcggc ctccatccga tgctcctcca cacgcccggc gcggagcggg cggaacgtcc
      601 cgggccccac gtgcagcgtg aggtagtgga catcgactcc gcagcgctcg agccgcgcga
      661 gcagctccgg cgtgaagtgc agtcccgcgg tgggcgccgc caccgagccg tcctcgcgcg
      721 cgtacaccgt ctggtagcgc tcacgatcct ccggcttcgg cgcgtcgtgc cggccgatgt
      781 agggcggcag cggcggcaac ccgtgccgct ccatcagctc ccgcaccggc cacggcgcct
      841 cgatcgccac gacgcgcgtc ccctcggagc cctcgcccac cacctgggcc aacgccgcgc
      901 ctccggccag cccgatgcgc gcgcctggcc ggaagcgacg gcccggtcgc accatcgcct
      961 cccatcgctc gtcgccgacc gggcgaatca gcagcagctc caccgcctgc ccgcccggct
     1021 ccgccacgcc caggagccgc gccggcatca cccggctccg attcgccacc aggcaatccc
     1081 cagcccgcag aagctccggc agctcggaga accggcgatc ctcccagccg cccgcggcgc
     1141 gatccagcac catcagccgc gaagcatccc gcggctccgc cggctcctgc gcaatcaact
     1201 ccggcggcaa ctcatagtcg aagagggacg tgtccatcga gattgaagtg tagcccacgc
     1261 gcgcccgact accagagaga gcaaccgagg aaagagaatg cttggccaac gagggtaacc
     1321 gggccgggga gaggcgcggg gcggggggca ccccctcgcc gggaccgtgt cttctgcgcg
     1381 ctgtcgaagg ctgagacctt cggtctcgtg gcccgatcga gacggcgcta cgggttgcga
     1441 ctcagcgtgt cccggcgagg ggtgcccccc gccccgcgcc tctccccggc ccgcctcgcc
     1501 cccgtgaggc ggctatccga tccgcgcccc gggcaacacc tcgaagtccg gccgcagcag
     1561 gataacctcg cccccttctc catacgcccc gagtacaagc acctccgaca cgaaggggcc
     1621 tatccgcttc ggcgggaagt tgacaaccgc caccacgcgc cggccgacga gcgcagccgg
     1681 cgtgtaccgg tcggtgatct gcgcgctcga ccgcttgacg ccgagcggcc cgaagtcgat
     1741 cgcgagcttg agcgccgggc gccgcgcctc cgggaactcc tgggcgtcga tcaccacgcc
     1801 cacgcgcatg tcgacgcgct cgaattcctc ccaggccagc gcccccccgg ggcggggctc
     1861 agcggacaat gggcggcgcg ccggagagga cgggcagcgt ggagagcgtc gcgcccggat
     1921 agtaatagag gacgatccgc tgcgcgttgt agccctggtc ggccatggtc ttggcgctcc
     1981 actggtcgag gccgaccccg tgcccgtagc cgcggcccac gaagcgcacc atcggcccca
     2041 ccaccgcgac cgcgaagagc gtgctcttga gcgtgtcgta ccccaccagg cgccgcagat
     2101 cgttgccccg cagagtcacg gtgcccgcgg tgccgcgcac cgcgatgcgg gccacccgga
     2161 gcgacacgct ccgctcgagg acgtcgaggg ccaccacgcg cccgatcgag atcccgcctc
     2221 gcagcagggc cgccgacagc tcggagagcg gcaggtccag gcgccagcgc tgatggggcg
     2281 actccgacgg gaactcgacg cggaccggct tgagcgcagg catgttcgag gccgcgaaga
     2341 ccacccgggg gtcctcggtg tagccgccgc tgtcggtgtg atagaaggcg gggaacagct
     2401 cgccctgcca caggaggacc tggcccgcgg tctcccggac cgcagtccac accaccgagt
     2461 cggccggcac ccggcccacg t
//
LOCUS       DATMCV010000433         2615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_818915, whole genome shotgun sequence.
ACCESSION   DATMCV010000433 DATMCV010000000
VERSION     DATMCV010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818915"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VNU03_10025"
     CDS             <1..1167
                     /locus_tag="VNU03_10025"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWP74844.1"
                     /translation="LTVLGVTGSVGRRTLELVEHFPEEFRVEGMAARGSNPELIVELC
                     RRHRPRALALSDAAAMDAVARALPTPRPELLAGPAGLVTLAREVEADVVLSAIVGGAG
                     LLPTMAAIQSGKSVALANKETLVMAGSLMTAAARERGVALLPVDSEHSAVFQCLEGHN
                     RSEIRRILLTASGGPFRTMKAADLARVTVAEALNHPTWRMGAKITVDSATLMNKGLEV
                     IEARWLFDVLPEQVQVVVHPQSVVHSLVEYVDGSVIAQLGVADMGIPILYALTYPQRL
                     PCPAERLDLTRVGSLTFEEPDAERFPCLALARRALLEGGCAPVILNAANEIAVAAFLE
                     GRIRFTQIPELIADALARVPARALDGIDTCVDVDTRTRATVREWLPGPVAAAAR"
     gene            1180..2538
                     /gene="rseP"
                     /locus_tag="VNU03_10030"
     CDS             1180..2538
                     /gene="rseP"
                     /locus_tag="VNU03_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HWP74845.1"
                     /translation="MATVQMLVSFVVVLGILIVVHELGHFLMARLAGVGVERFSIGFG
                     PVLWRFRGKETEYCVSAIPMGGYVKMMGDDENPLEGGKGASIDPAKAFNGKPLAARFL
                     IVFAGPAMNFVLAAVIAGLMFMLVGRPVAPALVGRVADGSPAAQAGLKTGDRLVAIDG
                     KPVQYWEDLARIVQAARGRALQVAVQGAAGERTVALTPAQTKRKDLFGDEQTVWEIGA
                     SPYIPAAIGDTVAGDPADQAGLKAGDVVATLEGQPVLSWDELAERIHQRAGQPTRLEV
                     KRGTETLAITVTPKKGKVPGADGKETEVGLVGIRPGGSVTMVRSNPIYALWEGVAWSG
                     DVTMKTAIGLYKIVVGQLDRSNIGGPIQIAKTAGEQARQGIVSLALFTAVISINLFLL
                     NLLPVPMLDGGHLLFFAFEAVLGRPLSVRKREVAQQVGFALLMLLMVFAFYNDFKRIG
                     LF"
BASE COUNT          361 a          886 c          975 g          393 t
ORIGIN      
        1 ctgaccgtgc ttggtgtcac cggctccgtg gggcggcgca cgctggagct ggtcgagcat
       61 ttcccggagg agttccgggt cgagggcatg gccgcgcgcg gctccaatcc cgagctgatc
      121 gtggagctct gccggcggca ccggcctcgc gcgctcgccc tgtcggacgc ggccgcgatg
      181 gacgcggtgg cccgggccct gcccaccccg cgccccgagc tgctcgcggg cccggccggc
      241 ctcgtgacgc tcgcccgcga ggtggaggcc gacgtggtcc tctcggccat cgtgggcggc
      301 gcgggcctgc tgccgaccat ggccgccatc cagagcggca agagcgtcgc gctcgccaac
      361 aaggagacgc tggtgatggc gggcagcctg atgaccgcgg cggcccggga gcgcggggtc
      421 gcgctcctgc cggtcgactc cgagcacagc gcggtcttcc agtgcctcga gggccacaac
      481 cgatccgaga ttcgccgcat cctgctcacc gcgtccggtg gtcccttccg gaccatgaag
      541 gcggcggacc tcgcccgggt gacggtggcg gaggcgctca accatcccac ctggcggatg
      601 ggcgccaaga tcacggtgga ttcggccacc ctcatgaaca aggggctcga ggtcatcgag
      661 gcgcgctggc tgttcgacgt gctgcccgag caggtccagg tggtggtgca tccgcagtcg
      721 gtcgtgcatt cgctggtcga gtacgtcgac ggctcggtca tcgcccagct cggggtggcc
      781 gacatgggca tcccgatcct ctacgcgctg acctacccgc agcggctgcc ctgtccggcc
      841 gagcggctcg acctcacgcg ggtcggctcg ctcaccttcg aggagccgga cgcggagcgg
      901 ttcccgtgcc tggccctcgc gaggcgcgct ctgctcgaag ggggctgcgc cccggtcatc
      961 ctgaacgcgg ccaacgagat cgcggtggcc gcgttcctgg agggccggat ccgattcacc
     1021 cagatcccgg agctgatcgc cgacgcgctg gcgcgggtgc ccgcgcgggc cctcgacggg
     1081 atcgacacgt gtgtggacgt cgacacccgg acgcgggcca cggtgcggga gtggcttccc
     1141 ggcccggtgg ccgcggcggc gagatagagg aggcgcttag tggcaacggt acagatgctg
     1201 gtgtcgttcg tggtggtgct cgggatcctc atcgtggtgc acgagctcgg ccacttcctg
     1261 atggcccggc tcgccggggt cggggtcgag cggttctcca tcggcttcgg cccggtgctc
     1321 tggcgcttcc gcggcaagga gaccgagtac tgcgtgtccg cgatcccgat gggcggctac
     1381 gtcaagatga tgggagacga cgagaacccg ctcgagggcg gcaagggcgc gagcatcgac
     1441 ccggccaagg ccttcaacgg caagccgctc gcggcgcgct tcctgatcgt gttcgcgggc
     1501 ccggcgatga acttcgtcct ggccgccgtg atcgcggggc tgatgttcat gctggtcggg
     1561 cggccggtcg cccccgcgct ggtggggcgg gtcgccgacg ggagccccgc cgcgcaggcc
     1621 ggtctcaaga ccggcgaccg cctggtcgcg atcgacggca agccggtgca gtactgggag
     1681 gatctcgccc gcatcgtgca ggccgcgcgc ggacgcgcgc tccaggtcgc ggtgcaggga
     1741 gcggcgggcg agcgcacggt ggccctcact ccggcgcaga ccaagcgcaa ggatctcttc
     1801 ggcgacgagc agaccgtctg ggagatcggc gcgagcccgt acatccccgc cgccatcggc
     1861 gacacggtgg cgggcgaccc ggcggaccag gccgggctca aggcgggcga cgtggtggcg
     1921 acgctcgagg ggcagcccgt cctctcctgg gacgagctgg ccgagcgcat ccaccagcgc
     1981 gcggggcagc ccacgaggct cgaggtcaag cgcgggaccg agacgctcgc gatcacggtg
     2041 accccgaaga agggcaaggt cccgggagcc gacggcaagg agaccgaggt cggcctggtc
     2101 ggcatccgcc ccggcggctc ggtgacgatg gtgcggtcga atccgatcta cgcgctctgg
     2161 gagggggtcg cgtggtcggg cgacgtcacc atgaagaccg cgatcgggct ctacaagatc
     2221 gtggtgggcc agctcgaccg gagcaatatc ggcggcccga tccagatcgc gaagaccgcg
     2281 ggggagcagg cgcgccaggg catcgtgagc ctagcgctct tcaccgcggt gatcagcatc
     2341 aacctgttcc tgctcaacct gctgcccgta cccatgctcg acggcggcca cctgctcttc
     2401 ttcgccttcg aggcggtgct tgggcggccg ctgtcggtgc gcaagcgaga ggtcgcccag
     2461 caggtcggct tcgccctgct gatgctcctc atggtgttcg cgttctacaa cgacttcaag
     2521 cggatcgggc tcttctgatg gggcagtacc agcggcacgt cttcgtgtgc acggtggggg
     2581 acagctgccc ggtccagggc gacgtggaga agtac
//
LOCUS       DATMCV010000434         2200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_291201, whole genome shotgun sequence.
ACCESSION   DATMCV010000434 DATMCV010000000
VERSION     DATMCV010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291201"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VNU03_10035"
     CDS             complement(<1..634)
                     /locus_tag="VNU03_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009479829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP74846.1"
                     /translation="MTSPTPVLDITQNENTAGRAGDKLKGRVAFVTGGTRGIGAAICR
                     SLASQGAILAAGFAGNDDAAKKFADAFSGTYATAVSIHKGNVGEAADCRRTIDEVIGS
                     HGRLDILVNNAGITIDKTVLKLSDEDWFKVLNVNLSGAFFLSQSALRHMAERGSGRIV
                     NVSSVIGEMGNIGQANYAASKSGLFGLTKTLAREAAFLLGKAGRLTDPDGV"
     gene            780..2063
                     /locus_tag="VNU03_10040"
     CDS             780..2063
                     /locus_tag="VNU03_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP74847.1"
                     /translation="MRLPIFYGWVVVAVVFVTMGVGINARTAFSLLFPPILDEFGWER
                     GVIAGAFSFGFLVSAALSPSLGRLMDRRGPRVVIELGVLLIGLGMLLATVVTRPWHLY
                     ATLGVMVGGGSVCIGYTGQSLFLPNWFVRRRGLAMSLAFSGVGVGSVILLPWMQTLIG
                     RSGWRTACWAMGLLVLLLLVPLNLLLRRRPEDMGLEPDGDRAIAGSTAVHPSNVVDAA
                     WAAVDWTLGRAARTARFWWIALGYFLGLFAWYAVQVHQTKYLIEIGFRPTDAAWALGV
                     VSLAGIPGQIALGWLSDRIGREWVWTVGALGFAICYATLLLLADSPTPTLLYVMVVSQ
                     GMLGYGLTSVVGAIPAEIFQGRHYGSIFGTLMLSSIGGGAVGPWLTGVLHDHTGNYVL
                     AFQIAIACSVVSAVAIWLAAPRKVRAVAGRLRARA"
BASE COUNT          242 a          796 c          776 g          386 t
ORIGIN      
        1 cgaccccatc cggatccgtg agccgacccg ccttgccgag caggaaggcc gcctcccggg
       61 ccagggtctt ggtgaggccg aacaggcccg acttcgacgc cgcgtagttg gcctgcccga
      121 tgttgcccat ctcgccgatg accgacgaga cgttgacgat gcgcccgctc ccccgctccg
      181 ccatgtggcg cagcgcggac tgcgagagga agaacgcgcc ggagaggttg acgttcagca
      241 ccttgaacca gtcctcgtcc gagagcttca gcaccgtctt gtcgatggtg atgcccgcgt
      301 tgttgaccag gatgtcgagc cggccgtggc ttccgatcac ctcgtcgatg gtgcgccggc
      361 agtcggccgc ctcgcccacg ttgcccttgt ggatgctgac cgcggtggcg taggtgcccg
      421 agaacgcatc cgcgaacttc ttggccgcgt cgtcgttgcc cgcgaagccg gcggccagga
      481 tcgcgccctg gctcgcgagg ctgcggcaga tcgcggctcc gatcccgcgg gtgccgccgg
      541 tcacgaaggc cacccggccc ttgagcttgt cgcccgcgcg gccggcggtg ttctcgttct
      601 gggtgatgtc gagcacggga gtcggggagg tcatggacgg tcctcacacg cgcggcggac
      661 gcgcgctccg cgtccacgcc gcgccgacgc gctgcgtagt gaaccaccac ggtttccgat
      721 cagccggcac atcgtggcaa atccccactg cggagtcaaa cggcgtacaa tcccccttcg
      781 tgcgcctgcc catcttctac ggctgggtcg tggtggcggt ggtcttcgtc accatggggg
      841 tggggatcaa cgcgcgcacc gcgttctccc tcctcttccc cccgatcctc gacgagttcg
      901 gctgggagcg cggggtcatc gcgggcgcct tctcgttcgg cttcctggtc tcggcggcgc
      961 tgagcccgtc gctgggccga ctcatggacc gccggggccc gcgcgtggtg atcgagctgg
     1021 gcgtgctact gatcggcctc gggatgctgc tggccaccgt ggtcacccgg ccgtggcacc
     1081 tctacgccac cctcggggtc atggtgggcg gcggcagcgt ctgcatcggc tacaccggcc
     1141 agtcgctctt cctgcccaac tggttcgtgc gccggcgcgg cctcgcgatg agcctcgcgt
     1201 tctcgggcgt gggagtgggc tcggtcatcc tgctcccgtg gatgcagacc ctgatcgggc
     1261 gctccgggtg gcgcaccgcc tgctgggcca tgggcctcct cgtcctgctc ctgctggtcc
     1321 cgctcaatct cctgctgcgc cgccgcccgg aggacatggg gctcgagccc gacggcgacc
     1381 gcgcgatcgc gggctcgacc gcggtgcacc cctcgaacgt ggtcgacgcg gcgtgggccg
     1441 cggtggactg gacgctcggc cgcgccgcgc gcaccgcccg cttctggtgg atcgcgctcg
     1501 gctacttcct cggcctcttc gcctggtacg cggtgcaggt gcaccagacc aagtacctca
     1561 tcgagatcgg cttccggccg accgacgcgg cgtgggccct cggcgtcgtg agcctggccg
     1621 gcatcccggg ccagatcgcg ctcgggtggc tctcggaccg gatcgggcgc gagtgggtgt
     1681 ggacggtggg cgcgctcggc ttcgcgatct gctacgcgac cctgctgctg ctcgccgact
     1741 cccccacccc cacgctcctc tacgtgatgg tggtgtcgca gggcatgctc ggctacgggc
     1801 tcacctcggt ggtgggcgcg atcccggccg agatcttcca gggccggcac tacggctcga
     1861 tcttcggcac gctgatgctc tcctcgatcg gcgggggcgc ggtcggcccg tggctcaccg
     1921 gcgtgctgca cgaccacacc ggcaactacg tgctggcctt ccagatcgcg atcgcctgca
     1981 gcgtggtctc ggcggtggcg atctggctcg cggcgccccg caaggtgcgc gcggtggccg
     2041 gccgcctgcg cgcgcgggcc tgaggcggcc atgtacgtcg acggctggct ctgggccttc
     2101 acccgcggcg tccgcctgcg gatcgcgggg gccaccgtgg ccgggctcct ctcggtgctc
     2161 gtgggcaccg cgcggctcgc cctgctcggc tgggtgctgg
//
LOCUS       DATMCV010000435         5618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_555650, whole genome shotgun sequence.
ACCESSION   DATMCV010000435 DATMCV010000000
VERSION     DATMCV010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555650"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1064)
                     /locus_tag="VNU03_10045"
     CDS             complement(57..1064)
                     /locus_tag="VNU03_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74848.1"
                     /translation="MSRPVIDVRDLRTHLVTGWGTVKAVDGVSFEVAEGETLGLVGES
                     GSGKSMTCLSLLRLVPRPAARILGGQILLDGEDLLAKSEAEMRKIRGRRIGMILQDPM
                     SSLNPVFSIGMQVREPVALYHGLRGSALAARAAELLAAVRIPSPAERLRAFPHQLSGG
                     MRQRVVGAMAIAAPPRLLIADEPTTSLDLTIQAQYLGLLEELQERHRLALVFVTHNLG
                     IVARICDRVAVMYAGRIVEMGPVRRLFTRPTHPYTRALLESIPRLGARAARLTAIEGQ
                     PPDLARLAAGCAFAPRCPSVMERCRVEAPPETVVEAGHVTRCWLDGGPEVGAPGGGTP
                     A"
     gene            complement(1078..1971)
                     /locus_tag="VNU03_10050"
     CDS             complement(1078..1971)
                     /locus_tag="VNU03_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74849.1"
                     /translation="MPAEPGALALPGVDVPAVPAARRGLRLPRFPLAIIGLFVLAAVV
                     APLLGLPDPHAQSLRERFKPPAWEEAGSWKHPLGTDRLGRDLLSRIVWGSRVSLAAGV
                     LTVLVASAFGAAVGLTAGYYGGRVDAVLMRATDATMSFPVILLALILAVTVGPSFTNV
                     VTAIAVILWARYARVIRGQVLTLMQLDFVAQARIAGAGAWRIITRHLLPNTLGTLLVL
                     VTLQIGYVIIVEASLSFLGAGIPPPTPAWGSMIAEGRDVVTRAWWVSLFPGLAILLVV
                     LAFNLLGDWLRDTFDPKLRQL"
     gene            complement(1961..2881)
                     /locus_tag="VNU03_10055"
     CDS             complement(1961..2881)
                     /locus_tag="VNU03_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74850.1"
                     /translation="MLRFVAARLVQSLVALAIISVVVFVLARATGDPLYLILPMSASQ
                     EDFDNARRYLGLDRPYVEQYLSFVGRAVTGDFGNSLRARRPVSELIRERLPNSLKLAL
                     FAMGVSLAMAFPLGIMAAVHKGTGLDRFAQIVSVLGQSLPTFWVAILLVEFVAGRLQW
                     LPAGGNEGFTSYVLPGFTLGWFVVAGLMRLLRSGMLEVLDSEYVKLARVKGMGEGRVI
                     WLHALKNALIPVITFAGIYFAILVTTAIVVETVFAWPGLGRLAYEGISSRDFPVIQAV
                     VLTTAAIVAVVNLCVDCLYAVIDPRIRYAR"
     gene            complement(3093..4679)
                     /locus_tag="VNU03_10060"
     CDS             complement(3093..4679)
                     /locus_tag="VNU03_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74851.1"
                     /translation="MKRASAMAPVFLLILALLGVTRPDASWAQPKTELTVALSSFATE
                     VLDPPLGGHLVKYYLSMIFDYLVGTTPDGQPSRDGGLATRWENSPDFKRWTFYLRKGV
                     KFHNGDEVTSEDVKFSIMRNIGKRSTTGYAGPLRTLIADIETPAPDRVVIVSKEPTLI
                     IPTYLSRSLSTEGMIVPRKYVETVGDDVFARKPIGSGPYKFVEQVTGSHIRLAAVDSH
                     WRIGTPKFKTMTFRLVPEETTRIALLRRGEVDVADVSRERVPELERESFPIHYRREEA
                     ILTMWWILGPDGPVAPTKDKRVREALNLAIDRAEIAQSIFGGKAEPASVPLGLSWSFK
                     DVGFKPTPEMVYPYDPARAKKLLADAGVASGFTLDVYAYQLPGLPEGKTMAEAIAGYW
                     EKLGIKTRLIPVDYPAFRKAWLDRATPGAVGYFNIANRDWIGAYALLEKMAYTPAKGN
                     ATVNDPEIDAMIAQVMRQTDRDKINALVRNVYTRLRSEHYGVPVVYLHSPYATSKSLG
                     KWNVGSVMYDLFFDVLASGK"
     gene            complement(4780..5451)
                     /locus_tag="VNU03_10065"
     CDS             complement(4780..5451)
                     /locus_tag="VNU03_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWP74852.1"
                     /translation="MALLAGSSEQHYRALVESSAGLICTHDMSGLLLSVNRAAADRLG
                     YAADELVGRSLGDLLAPSVQAHFPRYLERIAHGSGDEGLMLVVTRDGEERIWSYSNVR
                     CQEPGGSPYVLGHAHDITDIKRSDARAGEAEALRSVAQLALATAHEINNPLTVIIASL
                     QLMTARKQVPPEAAGYVDRAIRAGEQIRDIVRNMSRITRLELSRQSPQLPPVLDLRKS
                     SDPQD"
BASE COUNT          865 a         1959 c         2030 g          764 t
ORIGIN      
        1 cagcccgcgc cgcaccggga agtgcttggt gagcgcctcg acctcgagga gcgtgctcac
       61 gcgggtgttc cgcccccggg cgcgccgacc tccggccctc catcgagcca gcagcgcgtc
      121 acgtgcccgg cctccaccac cgtctcgggc ggcgcctcca cccggcagcg ctccatcacc
      181 gacgggcagc gcggcgcgaa ggcgcagccc gcggccagcc gcgccaggtc aggcggctgg
      241 ccctcgatgg cggtgagccg cgccgcgcgg gccccgaggc gcgggatgga ctcgagcaga
      301 gcccgggtgt agggatgcgt cgggcgcgtg aagagccggc gcaccggccc catctcgacg
      361 atgcgcccgg cgtacatcac cgccacccgg tcgcagatgc gcgccacgat gcccaggttg
      421 tgggtgacga agaccagcgc cagtcgatgc cgctcctgca gctcctccag gaggcccagg
      481 tactgcgcct ggatggtgag gtcgaggctc gtggtgggct cgtcggcgat cagcagccgc
      541 gggggcgccg cgatcgccat cgcccctacc acccgctggc gcatgccgcc cgagagctgg
      601 tgcggaaacg cgcgcagccg ctccgcgggc gacgggatgc gcaccgcggc gagcagctcg
      661 gcggcccgcg cggcgagggc actcccacgc agaccgtgat agagcgcgac cggctcgcgc
      721 acctgcatgc cgatcgagaa caccgggttg agcgagctca tcgggtcctg caggatcatc
      781 ccgatccgcc ggccgcggat cttccgcatc tcggcctcgc tcttggcgag caggtcctcg
      841 ccgtcgagca ggatctggcc gcccaggatg cgtgcggccg gccgcggcac caggcgcagc
      901 aggctcaggc aggtcatgct cttgcccgag ccggactccc ccaccaggcc gagggtctcg
      961 ccctccgcca cctcgaagga gacgccgtcc accgccttca cggtgcccca gccggtgacc
     1021 agatgggtgc ggagatcgcg aacgtcgatg accggacggg acacggcggg tcggcgctca
     1081 gagctgtcgc agcttggggt cgaaggtgtc gcgcagccag tccccgagca ggttgaaggc
     1141 gagcaccacc agcaggatcg cgagccccgg gaagagcgac acccaccacg cccgcgtcac
     1201 cacgtcgcgc ccctccgcga tcatggagcc ccacgccgga gtcggcggcg ggatgcccgc
     1261 gcccaggaag gagagcgagg cctccacgat gatgacgtag ccgatctgga gcgtgaccag
     1321 caccagcagg gtgccgaggg tgttgggcag caggtgccgc gtgatgatcc gccacgcccc
     1381 cgcccccgcg atgcgcgcct gggccacgaa gtcgagctgc atcagggtca gcacctggcc
     1441 gcggatgacg cgcgcgtagc gggcccagag gatcaccgcg atggcggtca ccacgttggt
     1501 gaagctcggg cccaccgtga ccgccaggat cagcgcgagc aggatcaccg ggaaggacat
     1561 ggtggcgtcg gtggcgcgca tcaggaccgc gtcgacgcgc cccccgtagt agccggcggt
     1621 caggccgacc gcggccccga aggcgctcgc caccagcacc gtgagcaccc ccgccgcgag
     1681 cgacacccgc gagccccaca cgatgcggga gagcaggtcg cggcccagcc ggtcggtccc
     1741 gagcgggtgc ttccagcttc ccgcctcctc ccacgcgggc ggcttgaagc gctcccgcag
     1801 cgactgcgcg tgcgggtcag gcaggccgag cagcggggcc accaccgcgg cgagcacgaa
     1861 gaggccgatt attgcgagcg ggaaccgggg caggcggagg ccacgccgcg ccgccggaac
     1921 cgccggcacg tccacgccgg gcagggcgag cgcgccgggc tcagcgggca tagcggatgc
     1981 gcgggtcgat gaccgcgtag aggcagtcga cgcacaggtt gaccaccgcc acgatggccg
     2041 cggtggtcag caccaccgcc tggatcaccg ggaagtcgcg cgagctgatg ccctcgtagg
     2101 cgaggcgccc gagcccgggc cacgcgaaga cggtctcgac cacgatggcg gtcgtcacca
     2161 ggatcgcgaa gtagatgccc gcgaacgtga tgaccgggat cagggcgttc ttgagggcgt
     2221 gcagccagat cacccgcccc tcgcccatcc ccttcacccg ggcgagcttg acgtactcgg
     2281 agtcgagcac ctcgagcatg ccggagcgga gcagccgcat gagcccggcc accacgaacc
     2341 agccgagggt gaagccgggc aggacgtagc tcgtgaagcc ctcgttgccg cccgcgggca
     2401 gccactgcag ccggccggcc acgaactcca ccagcaagat cgccacccag aaggtgggca
     2461 gcgactggcc cagcaccgac acgatctggg cgaaccggtc gagcccggtg cccttgtgca
     2521 ccgcggccat gatgccgagc gggaacgcca tcgcgagcga gacgcccatc gcgaagaggg
     2581 ccagcttgag ggagttgggc aggcgctccc ggatcagctc gctgaccggg cggcgcgcgc
     2641 gcagcgagtt gccgaagtcg ccggtgaccg cgcggcccac gaaggagagg tactgctcca
     2701 cgtagggccg gtccaggccc aggtagcggc gggcgttgtc gaagtcctcc tgggaggccg
     2761 acatcgggag gatcaggtag agcggatcgc cggtggcccg ggccagcacg aagaccacca
     2821 ccgagatgat ggcgagcgcc acgagcgact gcacgagccg ggccgcgacg aagcggagca
     2881 ttgcgggccg tctggcggct tagagcaaag cctttaacgg cttgttctgg atggggggga
     2941 gcggcggcgc tcctccccca tgccccgtgc cggtcactcg ctcggggcca cggccgcgcc
     3001 cattgttctg aatggggggg agcggcggcg ctcctccccc ataccccccc accgcacggt
     3061 tcatgcgccc ggggccgacc acacgcgctc ggttatttac ccgaggcgag cacgtcgaag
     3121 aagaggtcgt acatgaccga gcccacgttc cacttgccga ggctcttcga cgtcgcgtag
     3181 ggcgagtgca ggtacaccac cgggaccccg tagtgctcgc tgcgcaggcg ggtgtacacg
     3241 ttacgcacga gcgcgttgat cttgtcgcgg tcggtctggc gcatcacctg cgcgatcatg
     3301 gcgtcgatct cggggtcgtt gacggtggcg ttgcccttcg ccggggtgta ggccatcttc
     3361 tcgagcaggg cgtaggcccc gatccagtcg cggttggcga tgttgaagta gccgaccgcg
     3421 cccggggtcg cccggtccag ccacgccttg cggaacgccg ggtagtcgac cgggatcagc
     3481 cgcgtcttga tgccgagctt ctcccagtag cccgcgatcg cctccgccat cgtcttgccc
     3541 tcgggcagcc cggggagctg gtaggcgtag acgtccaggg tgaagccgga ggcgactccc
     3601 gcgtcggcga ggagcttctt ggcgcgcgcc ggatcgtagg ggtacaccat ctcgggcgtc
     3661 ggcttgaagc ccacgtcctt gaaggaccac gagagcccga gcggcaccga cgccggctcg
     3721 gccttgcccc cgaagatcga ctgggcgatc tcggcccggt cgatggccag gttgagggcc
     3781 tcgcgcacgc gcttgtcctt ggtgggcgcc accggcccgt cggggccgag gatccaccac
     3841 atggtcagga tggcttcttc gcggcggtag tgaatgggga agctctcccg ctccagctcg
     3901 ggcacccgct cgcggctcac gtcggccacg tcgacctcgc cccggcgcag gagcgcgata
     3961 cgggtggtct cttcgggcac gaggcggaag gtcatggtct tgaacttggg ggtgccgatg
     4021 cgccagtggc tgtcgaccgc ggcgagcctg atgtgggagc cggtcacctg ctcgacgaac
     4081 ttgtagggcc cgctgccgat cggcttgcgg gcgaagacgt cgtcgccgac cgtctccacg
     4141 tacttcctgg gcacgatcat cccctcggtc gagagcgagc gcgagagata ggtggggatg
     4201 atgagggtcg gctccttgct gacgatcacc acccggtccg gcgcgggggt ctcgatgtcc
     4261 gcgatcaggg tgcgcagcgg gcccgcgtag ccggtggtgg agcgcttgcc gatgttccgc
     4321 atgatgctga acttcacgtc ctcggaggtc acctcgtcgc cgttgtggaa cttgacgccc
     4381 ttccggaggt agaaggtcca gcgcttgaag tcgggcgagt tctcccagcg cgtcgcgagg
     4441 ccgccgtcgc gcgagggctg cccgtccggc gtggtcccca ccaggtagtc gaagatcatc
     4501 gagaggtagt acttcaccag gtggccgccc aggggcgggt cgagcacctc ggtggcgaag
     4561 ctcgagagcg ccaccgtgag ctcggtcttg ggctgggccc acgaggcgtc cggccgcgtc
     4621 acgccgagca gggccaggat cagcaggaac accggtgcca tcgcgctcgc acgcttcatt
     4681 gatcgctcct cgcgtcgggg gatcggctgg aaagtcctgc tctcgccgcc cagaccttag
     4741 cccgagaggg ggccggacgg caagcccttc cagccgcgat cagtcctgcg ggtcgctgga
     4801 cttgcggaga tcgaggaccg gcggcagctg cggcgactgg cgggacagct cgaggcgggt
     4861 gatccgggac atgttgcgca cgatgtcgcg gatctgctcg cccgcgcgga tggcgcgatc
     4921 cacgtagccg gcggcctccg ggggcacctg cttccgcgcc gtcatgagct gcaggctcgc
     4981 gatgatgacg gtcagcgggt tgttgatctc gtgcgcggtg gcgagcgcga gctgggccac
     5041 cgagcggagc gcctccgcct cccctgcgcg cgcgtccgag cgcttgatgt cggtgatgtc
     5101 gtgggcgtgg cccagcacgt agggcgaccc ccccggctcc tggcagcgca cgttgctgta
     5161 gctccagatg cgctcctccc cgtcccgggt caccaccagc atcagcccct cgtcgcccga
     5221 gccgtgggcg atgcgctcga ggtaccgggg gaagtgcgcc tgcaccgagg gcgccaggag
     5281 atcacccagg ctccggccga ccagctcgtc ggcggcgtaa cccaggcgat cggcggcggc
     5341 ccggttgacc gagagcagga ggcccgacat gtcgtgggtg cagatcaggc cggcgctcga
     5401 ctccaccagc gcgcgatagt gctgctcgga cgatccggcg agaagggcca tcggcgggct
     5461 ccgctaggcg ggcgagaacg cccgctgcac cgccccgatc aggtcggcga tggtgaacgg
     5521 cttggccagc acggggacgc cgctctggcg gaggaaggca tcgtactcct cggcctccgc
     5581 gtagccggtc acgaacacga gacgcccgtg cgcgctcg
//
LOCUS       DATMCV010000436         4083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_555695, whole genome shotgun sequence.
ACCESSION   DATMCV010000436 DATMCV010000000
VERSION     DATMCV010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555695"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2144
                     /locus_tag="VNU03_10070"
     CDS             <1..2144
                     /locus_tag="VNU03_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP74853.1"
                     /translation="ALDLDALTRQFLRHVAHGLGADTAGVWLLEEDRQWMTPFAGYHV
                     PAESLAALRDVRIRLDDDAFFGEAARRRQSMISTDATHDLRIAEAVRRAAPTRTLLFV
                     PILAQEQMIGGFVAGWWDAARDLSEAEMRLMEAVASQAGVALQNARLFRDNQRRVQEL
                     SVLHELSQTVTGQLDQSGLLDTVYQQIARVLEVRSMAVVLLDEEHDRLRVVLRVRDGR
                     RCDDEEPHFYPRDAAGLSGTVFETGRAVRTTDYAADCARHGVAPLAAAIGMPYCLIVP
                     MTAGDRVLGVLSLRSPDRPFTDADERLIANIAQLAALMLRSARLYEERSRAFGELASA
                     QDQLVRTEKLRALGEMASGVAHDFNNVLASILGRAQLLLERIQDVKLRRWLQVIERAA
                     MDGARTVRRLQDFTGIRRDQPAVAVDLNQVVQQVLEATESMWRQDARRRGVEIEVLTD
                     LAERLPQIAGDPAELREAFTNLVLNAVDAMPKGGQLTLRTSVPDGQVMVEVIDTGTGI
                     PEPIRQKIFDPFFTTKGPKGTGLGLSMAYGILARHSGRITVESEEGRGTTFRLSFPPS
                     DQVPEVAAEPTPAAASTVSLRCLVVDDEEEVAEVVADILTTAGHVAVIAASGQAAVDL
                     FSADPFDLVFTDLAMPGMTGWQVARAVKAKAPEVAVVMMSGFGVEVEPAELRNNGVDL
                     VLSKPLQIKDVLRAVATIQRADRGRGEAEAR"
     gene            2153..3217
                     /gene="trpD"
                     /locus_tag="VNU03_10075"
     CDS             2153..3217
                     /gene="trpD"
                     /locus_tag="VNU03_10075"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWP74854.1"
                     /translation="MITEALRSLVDRRDLSRLEAAAAMEAIMSGAATDAQIAAFLTAL
                     RMKGETVEELIGFAQVMRQKAVKVRTHGADLVAQTGTDREMLIDTCGTGGDASGTFNV
                     STATAFVVAGAGLKVAKHGNRSVSSLCGSADVVETLGISIELAPAKVARCIDELGIGF
                     LYAPLLHTAMKHVMAARREMGVRTVFNMLGPLTNPAGANAQVIGVYAPGLTEPLARVL
                     AELGTIRAFVVHGADGLDEISNTGESHIAEVREGVVRTTRVRPEDFGMPRAAIADLQG
                     GDRRENAQIIRGVLAGEPGPRRDIVLMNAAAALVAGAKARDLKEGVGLAAQSVDSGAA
                     AGKLEALIGLSHRLAAEPAA"
     gene            3372..>4083
                     /locus_tag="VNU03_10080"
     CDS             3372..>4083
                     /locus_tag="VNU03_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWP74855.1"
                     /translation="MASRVPRYHQIAQTLRERIVAEGRGPGQRLDTQRHLAREFGVTL
                     MTLRQALDLLERDGLIARRHGLGTFVARPAIDYDILHLRALAGDLSALGEDVATRFLR
                     SYTEPADSRVAEPLGLAERAQVFVLERLRLVDGEPVSFQASYLPAVLGIEVSKADLAV
                     TPLRQVLAFKLGIEITEARETVAAVSLERRAARELGCRTGIAAFRSDRVSVGADGTPV
                     VYDRVFIPGDRFRITRQLR"
BASE COUNT          568 a         1468 c         1485 g          562 t
ORIGIN      
        1 gcgccctcga tctcgacgcg ctcacccgcc agttcctccg ccacgtggcc cacggcctgg
       61 gcgccgacac cgcgggggtg tggctcctcg aagaggaccg gcagtggatg accccgttcg
      121 cgggctatca cgtgccggcg gagtcgctcg ccgcgctccg cgacgtgcgc atccgcctcg
      181 acgacgacgc cttcttcggc gaggcggccc gccgccgcca gtcgatgatc tcgaccgacg
      241 cgacccacga cctccgcatc gcggaggcgg tgcggcgggc ggccccgacc cgcacgctgc
      301 tcttcgtgcc gatcctggcc caggagcaga tgatcggcgg cttcgtggcc ggctggtggg
      361 acgcggcccg cgatctcagc gaggccgaga tgcggctgat ggaggcggtg gcgagccagg
      421 cgggcgtcgc gctccagaac gcgcgcctct tccgcgacaa ccagcgccgg gtccaggagc
      481 tgtcggtgct gcacgagctg tcgcagacgg tgaccggcca gctcgatcag agcgggttgc
      541 tcgataccgt ctaccagcag atcgcccgcg tcctcgaggt ccgcagcatg gcggtcgtcc
      601 tgctcgacga ggagcacgac cggctgcggg tggtcctgcg cgtgcgcgac gggcggcggt
      661 gcgacgacga ggagccccac ttctacccgc gcgacgcggc cgggctctcc ggcaccgtgt
      721 tcgagaccgg gcgggccgtc cgcaccaccg actacgcggc cgactgcgcc cggcacgggg
      781 tcgcgccgct cgccgccgcg atcggcatgc cctactgcct gatcgtgccg atgaccgcgg
      841 gcgatcgggt gctcggggtg ctctcgctgc gcagccccga ccggccgttc accgacgccg
      901 acgagcgcct catcgccaac atcgcccagc tcgcggccct catgctgcgg agcgcgcgcc
      961 tctacgagga gcgctcgcgg gccttcgggg agctggcgtc ggcgcaggac cagctggtgc
     1021 gcaccgagaa gctgcgcgcc ctcggcgaga tggcctcggg ggtggcccac gatttcaaca
     1081 acgtgctggc ctcgatcctg ggccgcgccc agctcctgct ggagcggatc caggacgtca
     1141 agctccgccg gtggctgcag gtcatcgagc gcgccgcgat ggatggggcc cgtaccgtgc
     1201 gccggctgca ggacttcacc ggcatccggc gggaccagcc ggcggtggcg gtggacctca
     1261 accaggtggt gcagcaggtg ctcgaggcca ccgagtcgat gtggcggcag gacgcccggc
     1321 gccgcggcgt cgagatcgag gtcctcaccg atctggccga gcgcctgccc cagatcgcgg
     1381 gcgatcccgc cgagctgcgc gaagccttca ccaatctggt gctcaacgcg gtggacgcga
     1441 tgccgaaggg cggccagctc accctgcgca cgagcgtccc ggacgggcag gtgatggtcg
     1501 aggtcatcga caccggcacc ggcatcccgg agcccatccg ccagaagatc ttcgacccat
     1561 tcttcaccac gaaggggccg aagggcacgg gcctcggcct ctcgatggcg tatggtatcc
     1621 tggcgcgcca cagcgggcgg atcaccgtcg agagcgaaga ggggcgcggc accacgttcc
     1681 gactgagctt cccgccgtcg gatcaggtcc cggaggtcgc ggccgagccg acgcccgccg
     1741 ctgcgtcgac cgtatcgctg cgctgcctgg tggtggacga cgaggaagag gtggccgagg
     1801 tggtcgcgga catcctgacc accgcggggc acgtcgcggt catcgcggcg agcgggcagg
     1861 ccgcggtgga tctcttctcg gccgacccgt tcgacctggt cttcaccgac ctggcgatgc
     1921 cgggcatgac cggatggcag gtggcgcgcg cggtgaaagc caaggcgccg gaggtcgcgg
     1981 tggtgatgat gagcggattc ggggtggagg tcgagccggc ggagctgcgc aacaacgggg
     2041 tcgacctcgt cctgtcgaag ccgctccaga tcaaggacgt gctgcgcgcg gtggcgacga
     2101 tccagcgcgc ggaccgcggg cgcggcgagg cggaggcccg gtgaccagcc cgatcatcac
     2161 cgaggcgctc cgctccctcg tcgaccggcg cgacctgagc cggctcgagg ccgccgcggc
     2221 catggaagcg atcatgtcgg gcgcggccac cgacgcccag atcgcggcct tcctgaccgc
     2281 gctccgcatg aagggcgaga cggtcgagga gctgatcggc ttcgcccagg tgatgcgaca
     2341 gaaggcggtg aaggtgcgca cgcacggcgc ggacctggtg gcccagaccg gcaccgaccg
     2401 cgagatgctg atcgatacct gcggcaccgg cggcgatgcg tccggcacct tcaacgtctc
     2461 caccgccacc gccttcgtgg tggcgggggc ggggctgaag gtggccaagc acggtaaccg
     2521 gtcggtgtcg tcgctctgcg gctcggccga cgtggtggag accctcggga tcagcatcga
     2581 gctggctccc gccaaggtcg cgcgctgcat cgacgagctc ggcatcggct tcctgtatgc
     2641 cccgctgctc cacaccgcca tgaagcacgt catggccgcg cggcgcgaga tgggggtccg
     2701 gaccgtcttc aacatgctgg gcccgctcac caacccggcg ggcgccaacg cccaggtgat
     2761 cggggtgtac gcgcccggcc tgaccgagcc gctcgcccgg gtgctggccg agctggggac
     2821 gatccgggcc ttcgtggtcc acggggccga cggtctcgac gagatctcca acaccggcga
     2881 gagccacatc gcggaggtgc gcgagggcgt ggtccgcacc acgcgggtgc gcccggagga
     2941 cttcggcatg ccgcgcgccg cgatcgccga cctgcaggga ggcgatcgcc gggagaacgc
     3001 ccagatcatc cgtggcgtcc tcgcgggcga gccgggcccg cgccgcgaca tcgtgctgat
     3061 gaacgcggcg gcggccctcg tggccggagc caaggcccgc gacctcaagg aaggggtcgg
     3121 tctcgccgcg cagtcggtcg atagcggcgc ggcagcgggc aagctcgagg ccctgatcgg
     3181 cctctcccac cgcctggccg cggagccggc cgcctgacct gcgggcattg accccggccg
     3241 cccggggcgg ttacaatgac cgcggtcgcg gtaactgccc gttgtgcctg catgattgcc
     3301 gacccgaagc tcgacgaggg cgttgagcgg ccaaccggag ttctagaaca gtgagctcga
     3361 gggagcgtcg cttggccagt cgcgtgccgc gctatcacca gatcgcccag acgcttcgcg
     3421 agcgcatcgt cgcggagggc cgggggcccg gccagcgcct cgacacccag cgccacctgg
     3481 ctcgcgagtt cggcgtcacg ctgatgaccc tccgccaggc gctggacctg ctcgagcgcg
     3541 acgggctgat cgcgcgtcgc cacggcctcg gcaccttcgt cgcgcgaccc gccatcgact
     3601 acgacatcct ccacctgcgg gcgctggccg gagacctgtc ggccctcggc gaggacgtgg
     3661 cgacccgctt cctgcgcagc tacaccgagc ccgcggattc gcgcgtggcc gagccgctcg
     3721 ggctcgccga gcgggcccag gtcttcgtgc tggagcggct gcgcctggtc gacggcgagc
     3781 cggtgagctt ccaggcctcg taccttcccg ccgtcctcgg gatcgaggtc tccaaggccg
     3841 atctggccgt gaccccgctg cgacaggtgc tcgccttcaa gctcgggatc gagatcaccg
     3901 aggcgcgcga gacggtcgcg gcggtgtccc tcgagcgccg ggccgcccgc gagctcggct
     3961 gccggaccgg catcgccgcg ttccgctccg atcgcgtatc ggtgggagcg gacgggaccc
     4021 ccgtggtcta cgaccgggtc ttcatcccgg gcgatcgctt ccgcatcacc cgccagctgc
     4081 gct
//
LOCUS       DATMCV010000437         2693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_758045, whole genome shotgun sequence.
ACCESSION   DATMCV010000437 DATMCV010000000
VERSION     DATMCV010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758045"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VNU03_10085"
     CDS             complement(<1..1113)
                     /locus_tag="VNU03_10085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP74856.1"
                     /translation="MFAGWIVVGVAFLIATFAFGIGFYGVGLYMVVLHDRLGWPVSLI
                     SMAITGYYVLSAAMITVVGDAFDRFGPRRVLLVAIGGLAGGVLLIASTARPWQLGLGL
                     AVMAVGWAGMSGAGINAIVAPWFERKRGLAVSMAMNGATCGGVLIVPLWAALIPRFGL
                     PGAALVVVGVMLVILVPLVGRFMHRGPEVLGLGPDGDSRPAAGAPAPTAPAGASGGVP
                     REGAAPLRRAALVRTRRFWTISAAFALGLLAQVGFITHQVAYLSPRLGREGAALAVSL
                     TTLAAIVGRLATGAFVDRIDRRRAASCNFALQALAVFAMIRWPSVPVLYLGCVIFGLG
                     VGNMTTFPSLIVQVEYPKEHFRRVVSLVVAINQFTFA"
     gene            1216..2643
                     /locus_tag="VNU03_10090"
     CDS             1216..2643
                     /locus_tag="VNU03_10090"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HWP74857.1"
                     /translation="MLDREICDRARTARDARFDGRFVSGVLTTRIYCRPVCPVRPARS
                     ANVIFFPTAAAAETAGFRPCLRCRPETAPGSAAWRGTAATVSRALRLIDQGALDEGHV
                     VDLAAALGVGTRHLTRLFLEHVGAPPTAAAYTRRVQLAKMLLSESRLSMIEIAFAAGF
                     RSVRRFNAVFRQTYRRPPSSIRRADSAALDGGGLTLRLAYCPPLGWAGTARRLAAECT
                     PGVEDIGPAGYRRTVAIDGRAGWLSVRPVAGLRQVELRLQLPDYWPLRAVIERVSRLL
                     DLDADPEAIARQLAVDGGLGMLRSRLRAVRLPGAWDGFEVAVHRVLRRHVGATGARDA
                     AGRLARALGRRLAAGPSAGPDRLFPEPEALAAAGPRVLGLSAAAARDLRRLGEATAGG
                     AIQFQTATFDALVAQLEREARFDGATAHWIAMRSLAEPDAVLGGPNGSAAASERWRPW
                     RSYVAICQDTAARATAESAPRGRRA"
BASE COUNT          364 a         1029 c          994 g          306 t
ORIGIN      
        1 cgcgaaggtg aactggttga tggccaccac gaggctcacc acccggcgga agtgctcctt
       61 cggatactcc acctgcacga tcaagctggg aaacgtcgtc atgttgccga ccccgagacc
      121 gaagatcacg cagccgagat agagcaccgg caccgacggc caccggatca tcgcaaagac
      181 cgcgagggcc tgcagggcaa agttgcacga ggcggcgcgc cgccgatcga tccggtcgac
      241 gaacgcgccg gtcgcgagtc ggcccacgat ggcggccagc gtggtgaggc tcaccgcgag
      301 cgcggcaccc tcgcgcccga gccgcgggct caggtaggcg acctgatgcg tgatgaagcc
      361 gacctgggcc agcagaccga gcgcgaaggc ggccgagatg gtccagaagc gccgcgtgcg
      421 aacgagggcg gcgcgccgga gcggggccgc gccctctcgc gggaccccac ccgacgcgcc
      481 ggccggtgcc gtcggtgccg gggccccggc ggccggccgg gagtcgccgt cgggtccgag
      541 gccgagcacc tcggggccgc ggtgcatgaa ccgccccacc agcggcacca ggatcacgag
      601 catgacgccc accaccacga gcgccgcgcc ggggaggccg aatcgaggga tcagcgcggc
      661 ccagagcggc acgatgagca cgccgccaca cgtggccccg ttcatggcca tgctgaccgc
      721 gagcccccgc ttccgctcga accacggcgc gacgatcgcg ttgatcccgg ccccgctcat
      781 gccggcccag ccgaccgcca tgaccgcgag gcccagcccg agctgccacg ggcgcgcggt
      841 cgacgcgatc aggagcaccc cgccggcgag cccgccgatg gccacgagca gcacgcgccg
      901 cggcccgaag cggtcgaacg cgtcgccgac cacggtgatc atggccgcgc tcagcacgta
      961 gtagccggtg atcgccatcg agatcagcga gaccggccat ccgaggcggt cgtggagcac
     1021 caccatgtag agaccaacac cgtagaaccc gatgccgaag gcgaacgtgg cgatcaggaa
     1081 cgcgaccccg acgacgatcc agcccgcgaa catgagcaga gcctgcccgc ggcggggccg
     1141 gcgggctggc cgctttcgga cgcggtcgtc cgggatgcgt ggcgaacggg gccgcggcgg
     1201 cgcacactgg acccgatgct cgatcgtgag atctgcgacc gcgcccgcac cgcccgggac
     1261 gcccgcttcg acgggcgctt cgtgagcggc gtgctgacca cgcgtatcta ctgtcggccg
     1321 gtctgcccgg tgaggcccgc ccggtcggcc aacgtgatct tcttccccac cgcggccgcc
     1381 gcggagaccg ccggctttcg tccctgcctg cgatgccgcc cggagacggc gccgggcagc
     1441 gccgcgtggc gcggcaccgc ggccaccgtc tcgcgtgcgc tgcgtctcat cgaccagggc
     1501 gcgctcgacg aggggcacgt cgtcgacctc gccgccgcgc tcggcgtcgg aacgcgccac
     1561 ctgacccggc tcttcctgga gcacgtgggc gctccgccga ccgccgcggc gtacacccgg
     1621 cgggtgcagc tcgccaagat gctgctcagc gagagccgcc tgtcgatgat cgagatcgcc
     1681 ttcgccgcgg gcttccggag cgtgcggcgc ttcaacgcgg tgttccgcca gacctaccgg
     1741 cgcccgccgt cgtcgatccg gcgcgcggac tccgccgcgc tcgacggggg cgggctcacg
     1801 ctgcgcctgg cctactgccc gccgctcggc tgggccggca ccgcgcgccg gctcgccgcg
     1861 gagtgcacgc cgggcgtcga ggacattggc cccgcgggct atcggcggac ggtcgcgatc
     1921 gacggacggg ccggatggct ctcggtgcgt ccggtggcgg gcctccgcca ggtcgagctg
     1981 cgcctgcagc tccccgacta ctggccgctc cgcgcggtga tcgagcgcgt atcgcgcctg
     2041 ctggatctgg acgcggaccc ggaggccatc gcccgccagc tcgccgtcga cggcggcctc
     2101 ggcatgctgc ggagccggct gcgcgcggtg cgcctgcccg gcgcgtggga cggattcgag
     2161 gtcgcggtcc accgggtgct gcggcgtcac gtgggcgcca cgggcgcgcg cgatgccgcc
     2221 ggccggctcg ctcgggcgct cgggcggcgg ctcgccgcgg gaccatccgc ggggccggac
     2281 cggctctttc cggagccgga ggcgctcgcg gcggcagggc cccgggtact cgggctgtcg
     2341 gccgcggcgg cgcgcgacct gcgccggctc ggcgaggcga ccgccggggg cgcgatccag
     2401 ttccagaccg cgaccttcga cgcgctggtc gcccagctcg agcgcgaagc ccgcttcgat
     2461 ggcgccaccg cgcactggat cgcgatgcgc agcctggccg agcccgacgc cgtgctcggc
     2521 gggccgaacg ggtcggccgc ggcctcggag cgctggcggc cctggcgctc ctacgtcgcg
     2581 atctgtcagg acaccgcggc acgagccacc gcagagtccg ccccgcgcgg gcgacgggcc
     2641 tgaccgctcg cgcccggctc agccgtcggc gcgcggcgcg ctgatcaacc cgg
//
LOCUS       DATMCV010000438         2307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_758063, whole genome shotgun sequence.
ACCESSION   DATMCV010000438 DATMCV010000000
VERSION     DATMCV010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758063"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VNU03_10095"
     CDS             <1..1557
                     /locus_tag="VNU03_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWP74858.1"
                     /translation="DDGDGIGDLRGILRRLPYLCELGVDAVWISPIFPSPMADFGYDV
                     SDYVGIDPLFGTLPELDALLAAAHAGGLRILLDLVANHTSDRHAWFQESRASRQSVRR
                     DWYIWRDPAPGGGPPNNWRSAFGGSAWELDPRTGQYYYHAFLAAQPDLNWRNPAVRAA
                     MHEVMRFWLKRGVDGFRVDVIWHLLKDDQFRDNPPNPAFRPGQPPDHAVIPVHTTDLP
                     EVHEVIAGLRQVVDEFPERVLIGEIYLPIDRLVTYYGRNLEGANLPFNFALLDAPWEA
                     RGIARLIDEYEAALPPGGWPNWVLGNHDRPRIAGRVGPAQARIAAMLLLTLRGTPTIY
                     YGDEIGMPQVPIPTDRVCDPFERNVPGIGCGRDGARTPMQWDATTSAGFSTGEPWLPV
                     AAGYARDNVELERRDPASMLELYRRLIAVRRRLPSLRLGHYRPIVASGDLLLYVRECP
                     GERTLVGLNLGGHPVPVTFPGRGLRGEVLVSSGADRDGAIGQGEVTLRGNEGLVMALA
                     PDAVVPPSVV"
     gene            1600..2076
                     /locus_tag="VNU03_10100"
     CDS             1600..2076
                     /locus_tag="VNU03_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HWP74859.1"
                     /translation="MRKRVIKPAAVEAPATDQEWLDVEELAQVEVTSEDAAHPIESAL
                     VPGDGAGWRAAQAGEQTVRLVFDRPQRLSRIWLLFIEPDTVRTQEFLLRWSPDGGKSF
                     REIVRQQWNFGPPETIREAEDYRVQLSGVTVLELAITPDKNRGDARASIAQWRLAG"
BASE COUNT          307 a          796 c          861 g          343 t
ORIGIN      
        1 gacgacggag acggcatcgg cgatctgcgg gggatcctcc ggcgcctgcc gtacctgtgc
       61 gagctgggcg tcgacgcggt ctggatctcg ccgatctttc cctcgccgat ggccgacttc
      121 ggctacgacg tctccgacta cgtcgggatc gatccgctct tcggcacgct ccccgagctc
      181 gacgcgctct tggccgccgc ccatgcgggc ggcctcagga tcctgctcga cctggtggcg
      241 aaccatacct ccgaccggca cgcgtggttc caggagagcc gcgcctcccg gcagagcgtg
      301 cggcgcgact ggtacatctg gcgcgacccg gccccgggcg gaggcccgcc caacaactgg
      361 cgctccgcct tcgggggcag cgcgtgggag ctggacccgc gcaccggcca gtactactac
      421 cacgccttcc tggccgcgca gcccgatctc aactggcgca acccggcggt gcgcgccgcg
      481 atgcacgagg tcatgcgctt ctggctgaag cggggggtgg acggcttccg ggtggacgtg
      541 atctggcatc tgctcaagga cgaccagttc cgcgacaatc ccccgaaccc ggccttccgg
      601 ccgggccagc cgccggacca cgcggtgatc ccggtccaca cgaccgacct gcccgaggtg
      661 cacgaggtca tcgcggggct ccggcaggtg gtggacgagt tcccggagcg ggtgctgatc
      721 ggcgagatct acctgccgat cgaccggctg gtgacctact acggccgcaa tctggagggc
      781 gcgaacctgc cgttcaactt cgcgctgctc gacgcgccgt gggaggcgcg cggcatcgcc
      841 aggctcatcg acgagtacga ggcggccctg ccgccgggcg gctggccgaa ctgggtgctc
      901 ggtaatcacg atcggccccg catcgccggc cgagtcggcc cggcgcaggc gcgcatcgcg
      961 gcgatgctgc tgctcacgct gcgcggcacg cccaccatct actacggcga cgagatcggc
     1021 atgccgcagg tgcccatccc gaccgaccgc gtgtgcgacc cgttcgagcg caacgtgccc
     1081 ggcatcggct gcggccggga cggcgctcgc acccccatgc agtgggatgc gacgacgagc
     1141 gcgggcttct ccaccgggga gccctggctg ccggtggcgg ccggctacgc gcgcgacaac
     1201 gtcgagctcg agcggcgcga cccggcgtcc atgctcgagc tctatcggcg gctgattgcg
     1261 gttcgccggc gcttgccgtc gctgcggctc gggcactacc ggccgatcgt ggcgagcggg
     1321 gacctgttgc tgtacgtgcg cgagtgcccg ggcgagcgca ccctggtggg gctgaacctg
     1381 ggcgggcacc cggtcccggt cacgtttccc ggcagggggc ttcgcgggga ggtcctggtg
     1441 tcgagcgggg cggatcgcga tggcgcgatc ggccagggcg aggtcacgct ccgcggaaac
     1501 gaagggctgg tcatggcgct cgcgccggac gcggtggtcc cgccgtccgt cgtctgatct
     1561 actggtggtc tactcgaatg cgaaggaggt accagagcga tgcgcaagcg tgtgatcaag
     1621 ccggcggccg tcgaggctcc ggccaccgat caggagtggc tggacgtgga ggaactcgcg
     1681 caggtggagg tcacgtcgga ggacgccgcc catcccatcg agtccgccct ggtgccgggc
     1741 gacggggcgg gatggcgggc ggcccaggcg ggcgagcaga ccgtgcggct cgtgttcgat
     1801 cggccgcagc gcctgagccg gatctggctg ctgttcatcg agcccgacac cgtgcgcacg
     1861 caggagttcc tgctgcgctg gtcaccggac ggggggaagt cgttccgcga gatcgtccgc
     1921 cagcagtgga acttcggccc cccggagacg atccgcgagg cggaggacta ccgcgtgcag
     1981 ctgtccggcg ttacggtgct cgagctggcg atcacgcccg acaagaatcg gggagacgcg
     2041 cgggcgtcga tcgcgcagtg gcgcctggcc ggctagctgc gcgtcgacgc cccccggtta
     2101 tctagcggtt atcgctactt ggtcggcgcc ggaagcggga tgcccggagt cgccgggttg
     2161 cacatcgagc tacccggccg ccgcgcctga cccggtgggc actgtacgcc cggggtcggg
     2221 tccgtcattc ccggggtgga gctgccgggc acgccggggg tcgtgctgcc gggcatgcgc
     2281 ggtgtcggag cgccgggcgt gctcggg
//
LOCUS       DATMCV010000439         2903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_686122, whole genome shotgun sequence.
ACCESSION   DATMCV010000439 DATMCV010000000
VERSION     DATMCV010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686122"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..696
                     /locus_tag="VNU03_10105"
     CDS             166..696
                     /locus_tag="VNU03_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74860.1"
                     /translation="MTTPGGSDLPLLERLAGLPAVSLSELVALLEGMPDLSREPLLAG
                     LVGTDEQGEPAAHPLLGLVLEHLKERPDGTCYAALLEALTGIWNAAVRGAVVARLRAS
                     GLPADDLLLRLQALSPTLGFQAEKREWARQWMADPTAQDGQLVQQCLVRLLLAIRGLA
                     EARARFLTDSGSTGPA"
     gene            746..1141
                     /locus_tag="VNU03_10110"
     CDS             746..1141
                     /locus_tag="VNU03_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007981659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWP74861.1"
                     /translation="MAGIPDGYKDILQKKAFANLATVSTDGSPQVTPVWVDYDGTHIR
                     FNTAKGRVKDQNLRRNPAVALSILDPDNPYRYLQVRGRVVEITEAGADAHIDSLAKKY
                     LGQDKYPYRKPGEVRVIYKVAPDRVQTMG"
     gene            1144..1488
                     /locus_tag="VNU03_10115"
     CDS             1144..1488
                     /locus_tag="VNU03_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP74862.1"
                     /translation="MIALDGVSKGYGGQQLLQDCTWRIGRGERIGLVGPNGAGKTTLC
                     RVLALVEETDAGRVHRDTGVTVGYLPQEVTTGEDRTVLAEALAASTRSGASRASSRRS
                     RPAWQAPAPTPD"
     gene            1458..>2903
                     /locus_tag="VNU03_10120"
     CDS             1458..>2903
                     /locus_tag="VNU03_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWP74863.1"
                     /translation="MAGPGADPGLTDTYGEVQHRFEALGGYRLEAEAKIILGGLGFSP
                     EEVHRPLAEFSGGWRMRAALARLLLLRPDLLLLDEPTNHLDLESLGWLENFLAAYEGS
                     VVIVSHDRYFLNRMVTAIADLTGGSVTLYHGDYDHFLVEREARQALREAQARNQAKRV
                     AEIERFIDRFRYKASKARQVQSRVKMLDRMERIETEAQARRIHFSFPQPPRTGRLVAR
                     LAGVRKAYGDNVVYSGVDFLVERGDRVALVGVNGAGKSTLLKVLAGALPFDAGERALG
                     SHVEVQYYAQHQLDLLDPTRTVLEEIELAAPEAQISRLRTILGSFLFSGDTVEKKVAV
                     LSGGEKARLALAKMLARPAALLCMDEPTNHLDLASKEVLEEALSGFTGTIVFISHDRY
                     FINRIATQVVEVDHGHLTTHLGTYDDYLDHKAKAVATPEPPETPRSAAPKAKPADHKP
                     GRRISQPASTDSTSGSTAAGGSAGAISGPPQM"
BASE COUNT          438 a         1027 c         1031 g          407 t
ORIGIN      
        1 cggccggcgc tggctcatgc cgtgggcggc gtgttcgtgg tggggctggt gatcgcggcg
       61 atcgccctgc tgtccgccct cctggtcccc gcggggcgag cgcgcgacct cgccatccca
      121 cgggaccgcg ccgcccggcc cggcgcctga tccgggggca gcccagtgac caccccgggc
      181 ggctccgatc tccctctcct ggagcggctg gccggcctgc ccgcggtgtc gctctccgag
      241 ctggtggccc tgctggaggg catgcccgac ctgtcgaggg agccgctcct ggccgggctc
      301 gtcgggacgg atgaacaggg ggaaccggca gcccatccgc tactcgggct cgttctcgag
      361 catctcaagg agcgaccgga cgggacgtgt tacgcggcct tgctggaggc gctgaccggg
      421 atctggaacg cggcggtgcg gggcgcggtc gtggcccggc tgcgggcctc cgggctcccc
      481 gccgacgacc tgctgctgcg cctgcaggcg ctgtcgccga cgctcggctt ccaggccgag
      541 aagcgcgagt gggcccgcca gtggatggcg gacccgaccg cccaggacgg gcagctggtg
      601 cagcaatgcc tggtgcggct gctgctcgcg attcgcgggc tggcggaggc ccgggcgcgg
      661 ttcttgacgg acagcgggtc cacggggccc gcctgagcat ggcaccctga cgagcagatc
      721 gaccgacgag aaaaggagga atctcatggc cggcattccc gacggataca aggacatcct
      781 gcagaagaag gctttcgcca acctggcgac ggtgagcacg gacggctcgc cccaggtcac
      841 cccggtctgg gtggactacg acggcaccca catccggttc aacaccgcga aggggcgggt
      901 gaaggatcag aacctgcgtc gcaacccggc ggtggccctg tccatcctgg atccggacaa
      961 cccctatcgc tacctccagg tgcgcggtcg agtggtcgag atcaccgagg cgggggccga
     1021 cgcccacatc gactcgctcg ccaagaagta cctcgggcag gacaagtacc cctaccggaa
     1081 gcccggtgag gtgcgcgtca tctacaaggt cgctcccgac cgcgtccaga cgatggggta
     1141 gacatgatcg ctctcgacgg ggtctccaag ggctacggcg gtcagcagct gctccaggac
     1201 tgcacgtggc gcatcgggcg gggcgagcgg atcggcctgg tcgggccgaa cggggccggg
     1261 aagaccacgc tctgccgcgt cctcgccctg gtggaggaga ccgacgcggg gcgggtccac
     1321 cgtgacaccg gggtcaccgt tggctacctc ccgcaggagg tcaccaccgg cgaggaccgc
     1381 accgtgctgg cggaggcgct cgcggcttcg acgaggtctg gcgcctcgag agcgagctcg
     1441 aggcgctcgc ggcccgcatg gcaggccccg gcgccgaccc cggattgacc gacacctacg
     1501 gcgaggtcca gcaccggttc gaggccctcg gcggctaccg gctcgaggcc gaggccaaga
     1561 tcatcctggg cgggctcggg ttctcgccgg aggaggtgca ccggccgctc gccgagttct
     1621 cgggcggctg gcgcatgcgc gcggcgctgg cgcggctcct gctgctgcgg cccgatctgc
     1681 tgctgctcga cgagcccacc aaccacctgg acctcgagtc gctcgggtgg ctcgagaact
     1741 tcctcgccgc ctacgagggc agcgtggtca tcgtctcgca cgaccgctac ttcctgaacc
     1801 gcatggtcac cgcgatcgcc gatctgacgg ggggctcggt cacgctctac cacggcgact
     1861 acgaccactt cctggtggag cgcgaggccc ggcaggccct gcgggaggcg caggcgcgca
     1921 accaggccaa gcgggtggcc gagatcgagc gcttcatcga ccggttccgc tacaaggcga
     1981 gcaaggcccg gcaggtgcag agccgggtca agatgctcga ccggatggag cgcatcgaga
     2041 ccgaggcgca ggcgcgccgc atccacttct cgtttccgca gccgccgcgc accgggcggc
     2101 tggtggcccg gctcgccggg gtgcgcaagg cgtacggcga caacgtggtc tattcgggag
     2161 tggacttcct ggtcgagcgc ggcgatcgcg tggccctggt gggcgtcaac ggggcgggga
     2221 agtcgacgct gctcaaggtc ctggcggggg cgctgccctt cgacgcgggt gagcgggcgc
     2281 tcggcagtca cgtggaggtg cagtactacg cccagcacca gctcgacctg ctcgacccca
     2341 cgcggaccgt cctcgaggag atcgagctgg ccgctcccga ggcgcagatc tcccgcctcc
     2401 ggaccatcct gggcagcttc ctcttcagcg gggacacggt ggagaagaag gtcgcggtgc
     2461 tgtccggcgg cgagaaggcc cgcctcgcgc tcgccaagat gctcgcgcgg cctgccgcgc
     2521 tcctgtgcat ggacgagccg accaaccacc tggacctcgc gtccaaggag gtcctggagg
     2581 aggccctctc gggcttcacc ggaaccatcg tcttcatctc gcacgaccgc tacttcatca
     2641 accgcatcgc cactcaggtg gtcgaggtgg atcacggcca cctcaccaca cacctcggca
     2701 cctacgacga ctacctcgac cacaaggcca aggccgtcgc caccccggag ccgcccgaga
     2761 ccccacgctc ggcggcgccg aaagcaaagc ctgcggatca caagcctggt cgccgaatct
     2821 cccaaccggc ctccaccgat tccacctccg ggtcgaccgc ggccgggggg agtgcggggg
     2881 ccatttctgg gcccccgcag atg
//
LOCUS       DATMCV010000440         2289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_222844, whole genome shotgun sequence.
ACCESSION   DATMCV010000440 DATMCV010000000
VERSION     DATMCV010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222844"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1911)
                     /locus_tag="VNU03_10125"
     CDS             complement(<1..1911)
                     /locus_tag="VNU03_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWP74864.1"
                     /translation="MGRSARFRVRYAVAVACAVAAILVRMSLTAVWGLKFPYLTFHPA
                     VLLSAWVGGLGPGLVTTGLCALAAVYFWIPPTSSFLIDTFGDRISLAGFLFIGAAISV
                     LNEMLQRRERYANAIMESITDGFAIVDGRWRLQFLNEQVVRLARRGRRELTGRSIWEV
                     FPELGGTSVETEARRAVAEETPRHTQVFTPSLGGWVDIQMYPSREGLALYIRDVSGQR
                     QAELVSSRLAAIVESSDDAIVSKDLTGTILSWNGAAERMFGYPAAEAVGRHITLIIPA
                     ERHHEEDTVLARIRSGQSVDHFETVRLHKDGRPVEVSLTVSPIRDAGGKVVGASKIAR
                     DIGIRKRIDRERLEFLTREQAARAEAEAANRAKDDFLTTLSHELRTPLNAVYGWAAML
                     RTGQVDEATTTRAIETIMRNANVQVQLIDDMLDVSRIASGKLRLDLQNIDLGAVIDAA
                     ADTVRPAALAKGIRLDVVREPSTGPTTGDPNRLQQVVWNLLSNAVKFTPREGRVQVHL
                     RRAHSHLEIVVADTGGGIAADFLPYVFERFRQGDGSSTRPHGGLGLGLTLVRHLVELH
                     GGSVSAESPGLGHGATFTVKLPVSATLIPIGATATPPSSPAPLPVAPLVHLEGIRVLV
                     VDDDLDGLTLVTE"
     gene            complement(1935..>2289)
                     /locus_tag="VNU03_10130"
     CDS             complement(1935..>2289)
                     /locus_tag="VNU03_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HWP74865.1"
                     /translation="GITTRKEFQLGINISGYGVDDPDAILFENYTCTSLRNYTSYCDE
                     GVSRLIEQQSQELDLRKRVALVHEIQKKLTEAGARPTVAWGVDFFAHWPYVKNLVPHH
                     SLYSFGRMQNVWMDR"
BASE COUNT          363 a          768 c          806 g          352 t
ORIGIN      
        1 ctccgtgacg agggtcagcc cgtcgaggtc gtcatcgacg accagcaccc ggattccctc
       61 gaggtggacg agcggggcca ccggcagcgg cgcggggctg gacggcggcg tcgcggtcgc
      121 tccgatcgga atcagggtgg cgctgaccgg gagctttacg gtgaaggtcg ccccatggcc
      181 gaggcccgga ctctcggccg agaccgagcc cccgtggagc tcgacgagat gcctgacgag
      241 ggtcagcccg aggccgagtc cgccgtgcgg gcgcgtgctg gagccgtcgc cctggcggaa
      301 gcgctcgaac acgtacggca ggaagtccgc cgcgatgccg ccgccggtgt cggcgacgac
      361 gatctcgagg tgggaatgtg cccgccgcag gtgcacctgg acgcggccct cccgcggggt
      421 gaacttcacc gcattgctga gcagattcca caccacctgc tggagccgat tcggatcccc
      481 ggtcgtcggt ccggtgctgg gctctcgaac cacgtcgagg cggatgccct tggcgagggc
      541 ggcgggacgc accgtgtccg ccgcggcgtc gatgacggcc cccaggtcga tgttctgcag
      601 atccaggcgg agcttgccgg acgcgatgcg gctcacgtcc agcatgtcgt cgatgagctg
      661 gacctggacg ttggcgtttc gcatgatcgt ctcgatggcc cgggtggtcg tggcctcgtc
      721 cacctgaccg gtccgcagca tggcggccca gccgtagacc gcattgagcg gcgtccgcag
      781 ctcgtgcgac agcgtggtca gaaaatcgtc cttggcccgg ttcgcggcct ccgcctcggc
      841 gcgggccgcc tgctcccgtg tgaggaactc gagccgctcc cgatcgatgc gcttgcggat
      901 gccgatgtcc cgcgcgatct tcgaggcccc gacgaccttg ccgcccgcat ctcggatcgg
      961 cgagacggtg agtgacacct cgaccggccg cccgtccttg tgcagccgaa ccgtctcgaa
     1021 gtgatcgacg gactggccgc tccggatccg cgccagcacc gtgtcctcct cgtggtgacg
     1081 ctcggccggg atgatcaggg tgatgtgccg gcccaccgcc tcggcggccg gatagccgaa
     1141 catgcgctcc gccgcgccat tccacgagag gatcgtgccg gtgaggtcct tgctgacgat
     1201 cgcatcgtcg gaggattcga cgatcgcggc caggcgggac gacaccagct cggcttgcct
     1261 ctggcccgag acgtcccgga tgtagagcgc gagtccctcc cgggagggat acatctggat
     1321 gtccacccag ccgcccaggg acggagtgaa cacctgggtg tgccgcggag tctcctccgc
     1381 gaccgcgcgg cgggcctccg tctccacgct ggtaccgccg agctccggga agacctccca
     1441 gatcgaccgg ccggtcagct cccggcgccc ccggcgtgcg agccgcacga cctgctcgtt
     1501 gaggaactgg agccgccacc gcccgtcgac gatggcaaac ccgtcggtga tgctctccat
     1561 gatggcgttc gcgtagcgct cgcggcgctg caacatctcg ttcagcacgc tgatggcggc
     1621 cccgatgaag aggaagccgg cgagcgagat gcggtccccg aaggtgtcga tcaggaagga
     1681 gctggtggga gggatccaga agtagaccgc ggccagggcg cagagaccgg tggtgaccag
     1741 gcccggcccg agcccgccga cccaggccga gagcaggacc gccggatgga aggtgagata
     1801 gggaaacttg aggccccaga ccgccgtgag agacatgcgg acgagaatcg cggcgacggc
     1861 acacgccacc gcgacggcat accgaacccg gaacctggcg gatcggccca tggggggaag
     1921 ctgacccgcg atcgctaccg gtccatccac acgttctgca tgcgcccgaa gctgtagagc
     1981 gagtggtggg gcaccaggtt cttcacgtac ggccagtggg cgaagaagtc gactccccag
     2041 gccaccgtgg gacgggcccc cgcctcggtg agcttcttct ggatctcgtg gacgagggcg
     2101 acccgcttgc ggagatcgag ctcctgtgat tgctgctcga tgagccgcga cacaccctcg
     2161 tcgcagtagc tggtgtagtt ccggagcgag gtgcacgtgt agttctcgaa caggatcgcg
     2221 tcggggtcgt ccaccccgta gcccgagatg ttgatcccga gctggaactc cttgcgagtg
     2281 gtgatcccg
//
LOCUS       DATMCV010000441         5422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_291582, whole genome shotgun sequence.
ACCESSION   DATMCV010000441 DATMCV010000000
VERSION     DATMCV010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291582"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VNU03_10135"
     CDS             <1..211
                     /locus_tag="VNU03_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWP74866.1"
                     /translation="LTVTALEKTGVELTTDNFVRAMESIKGYHDIFNGPEVNFAADKH
                     QGADSSFLAVVKGGRWTRLTDPLRF"
     gene            complement(273..965)
                     /locus_tag="VNU03_10140"
     CDS             complement(273..965)
                     /locus_tag="VNU03_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HWP74867.1"
                     /translation="MDLVAGVNWRFKRHAGGEGRPPFYDIDATYPSLRILDRAHPVIS
                     AEMEAVLHGKDRIPRYHDLDRAESYISGSDDPEKNWRVFMLWSMAGTPKANQARCPRT
                     TALLRRIPNLYQAFFSILDAGKSIPAHSGSYLGYLRYHLGLHVPATNPPTMRVKDQYH
                     TWEAGRSILFDDSWNHEVVNRSDDIRVVFIVDVLRPLPFHLHAANWILTRLLMRHSEE
                     ARQVLANIKKFS"
     gene            complement(965..1837)
                     /gene="rfbA"
                     /locus_tag="VNU03_10145"
     CDS             complement(965..1837)
                     /gene="rfbA"
                     /locus_tag="VNU03_10145"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HWP74868.1"
                     /translation="MKGIILAGGSGTRLYPATRGISKQLVPIYNKPMVYHPLSTLMLA
                     GIREILLITTPEDAPAFHRLFGDGKDLGLALSYAVQPRPEGLAQAFIIGRQFVGRDAV
                     ALALGDNVFYGHNLPVALQAAVARPAGATVFAYRVRDPSRYGVVTFGPDGRAREIEEK
                     PAQPRSSFAVTGLYFYDNRVLDIAAGLKPSARGELEITDVNRAYLERGELHVERLGRG
                     IAWLDTGTYDSLLQASNFIQAIEERQGLMVACVEEIAWRMGWITAGDIRRIAAPMKQN
                     GYGQYLLGLLESDA"
     gene            1955..3481
                     /locus_tag="VNU03_10150"
     CDS             1955..3481
                     /locus_tag="VNU03_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74869.1"
                     /translation="MRVLGAILTLVLVVMLAPGLAGAVPPDGTMAIGVHVTLVSRWLD
                     PGETEALITPFMVLYALHDALVKPMPGGIMTPSLAESWSASKDGLSYEFVIRKNARFH
                     TGDPVTAEDVKFTFERYKGSGAKLLKDKVKEIQVLAPNRVRFVLKEPWPDFMAFYGTS
                     ATGAGWIVPKKYIEKVGEEAYKRAPVGAGPFKFVSFTPGIELTLEAFGDYWRKAPQVK
                     RLIMRSIPDETTRAAAVKTGEVDAAYLFVGPVAEDLKRSPGVQVVAPLLYGIYWLDFL
                     DQWDPKSPWHDRRVRQAASLVIDRNAINQAEMLGLGKAAGAFVPPEFDFALRVDPPAF
                     DPKRAKQLMVEAGYPNGFDAGDLTPLPPYTTVAEAVGGNLQAIGIRTSVRTMERATFL
                     TAWREKKLRGLLIGATGAAGNAATRLEAFFTKGGTYPYGTRPEIDDLFQRQAAENDRK
                     QREALLHQIQKIVADQTLVAPIFQQAFLWGVGSRVEQPAAGLIQGYPYAAPCEDLKLK
                     "
     gene            complement(3494..4366)
                     /locus_tag="VNU03_10155"
     CDS             complement(3494..4366)
                     /locus_tag="VNU03_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWP74870.1"
                     /translation="MSPTSRPVAQVVRPEPVVEGAGVHLRRSIGTRRLDHLDPFLLLD
                     HFESVRPEDYEPGFPYHPHRGIETVTFVRTGEVQHRDSLGHRGSIGPGDIQWMTSGSG
                     ILHEEMPQVRPEGIGGLQLWLNLPAREKMTRPKYRDLAGSHLAESALDGGARARVIAG
                     DAADGRLIGPVEGLAVAPKFLDVTVPEGATFRETVPRGHTVFAYVDQGEVRFGPDRKA
                     AHAPALVVFGDGDLVEAAGAPGGGRFLLAAARPLGEPIARHGPFVMNTTAEIEEALRD
                     LRSGHFIRDDPRDA"
     gene            complement(4378..4815)
                     /locus_tag="VNU03_10160"
     CDS             complement(4378..4815)
                     /locus_tag="VNU03_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HWP74871.1"
                     /translation="MSTPRPASGSRPPGWQQEHARRYVESGGKDGHVWEGVTTLLLTT
                     TGRRSGQARTTPLIYGRDGDRYVVVASRGGAPQHPAWYENLAAHPEVTVQVMADRFKA
                     RARTASAAERPALWKTMAAIWPPYDEYQAKTSRTIPVVILERA"
     gene            complement(4891..5352)
                     /locus_tag="VNU03_10165"
     CDS             complement(4891..5352)
                     /locus_tag="VNU03_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWP74872.1"
                     /translation="MASGYHRIVVGVDGSPHSRRAVALVARLAPPRGGRVTCVRVIEA
                     VRLPSMPFVPARMRAQLETQAAAMDRSRRAMARRQLEAAVGALAKAGWRARGVVRAGP
                     PLPELLAAVRAERADLLVLGARGAGAVRHFLLGSVVDAALRRSPVAVLVAR"
BASE COUNT          770 a         1970 c         1909 g          773 t
ORIGIN      
        1 cctcaccgtg accgcgctcg agaagacggg ggtcgagctg accaccgaca acttcgtgcg
       61 ggccatggag tcgatcaagg gctaccacga catcttcaac ggcccggagg tcaacttcgc
      121 ggccgacaag caccagggcg ccgattcgtc cttcctcgcg gtggtgaagg gcggacgctg
      181 gacgcgcctg acggacccgc tgcgcttctg atccgacggt gagcggcgac ggcgcccgcg
      241 gatccggggc gccgtcgccg gtcacctccg cctcaggaga acttcttgat gttggccaac
      301 acctgccgcg cctcctcgct gtggcgcatg agcaggcggg tcaggatcca gttggccgcg
      361 tggaggtgga acggcaacgg tcgcagcacg tccacgatga acaccacccg gatgtcgtcg
      421 ctccggttga ccacctcgtg gttccagctg tcgtcgaaga ggatgctccg cccggcctcc
      481 cacgtgtggt actgatcctt gacccgcatc gtcggcgggt tggtcgccgg cacgtgcagc
      541 cccaggtgat agcgcaggta gcccaggtag ctcccgctgt gggcgggaat ggacttgccc
      601 gcgtcgagga tcgagaagaa ggcctggtag agattcggga tccggcgcag cagcgcggtg
      661 gtgcgcgggc agcgcgcctg gttggccttc ggggtgccgg ccatggacca cagcatgaag
      721 acccgccagt tcttctccgg atcgtcgctg cccgagatgt agctctcggc ccggtccagg
      781 tcgtggtagc gcgggatccg gtccttcccg tggaggaccg cctccatctc cgcgctgatc
      841 accgggtggg cgcggtccag gatccggagt gacgggtagg tcgcgtcgat gtcgtagaac
      901 gggggccgcc cctccccgcc cgcgtgccgt ttgaagcgcc agttgacccc cgcgaccagg
      961 tccatcacgc gtcgctctcc agcaggccca ggaggtactg cccgtagcca ttctgcttca
     1021 tcggagccgc gatccgccgg atgtcgcccg cggtgatcca gcccatgcgc cacgcgatct
     1081 cctccacgca cgccaccatg aggccctgcc gctcctcgat ggcctggatg aagttggagg
     1141 cctggagcag ggagtcgtag gtcccggtgt cgagccacgc gatgccgcgg ccgaggcgtt
     1201 cgacgtgcag ctcgccgcgc tcgaggtacg cgcggttgac gtcggtgatc tcgagctccc
     1261 cgcgggccga cggcttgagc ccggccgcga tgtcgaggac ccggttgtcg tagaagtaga
     1321 gcccggtcac cgcgaagctg gagcgcggct gggccggctt ctcctcgatc tcccgcgcgc
     1381 ggccgtcggg cccgaacgtc accaccccgt agcgcgaggg atcgcgcacc cggtaggcga
     1441 acacggtggc gcccgccgga cgggcgaccg ccgcctggag cgccaccggc aggttgtgcc
     1501 cgtagaagac gttgtcgccc agggccaggg ccaccgcgtc gcgaccgacg aactgacggc
     1561 cgatgatgaa ggcctgggcc agcccctcgg gccggggctg caccgcgtag gagagcgcca
     1621 ggccgaggtc cttgccgtcc ccgaagagcc ggtggaacgc gggtgcgtcc tccggggtcg
     1681 tgatcagcag gatctcccgg atgcccgcca gcatgagggt ggacagcggg tggtacacca
     1741 tcggcttgtt gtagatgggc accagctgct tgctgatccc ccgggtcgcg gggtagagcc
     1801 gcgtcccgga cccgccggcg aggatgatcc ccttcatcac gtcatcgtaa gcgggccggc
     1861 attgtcgccg cctgggtttt ttcaagccgt cggagacggc cggaccgaag ccgtggtacc
     1921 ttctcgctca cccaaccttc cccggaggtg agcgatgcgt gttctcggcg cgatcctgac
     1981 cctcgtcctc gtcgtcatgc tggctcccgg cctcgcgggc gcggtcccac ccgacggcac
     2041 catggccatc ggcgtgcacg tgacgctcgt ctcgcgctgg ctcgatccgg gcgagaccga
     2101 ggcgctcatc accccgttca tggtgctcta cgccctgcac gacgcgctcg tgaagcccat
     2161 gccgggcggc atcatgactc cgagcctggc cgagtcctgg agcgcgtcca aggacggcct
     2221 cagctacgag ttcgtcatcc ggaagaacgc gcgcttccac accggggacc cggtcaccgc
     2281 cgaggacgtg aagttcacgt tcgagcgcta caagggcagc ggggccaagc tgctcaagga
     2341 caaggtcaag gagatccagg tgctcgcgcc gaaccgggtc cgcttcgtgc tcaaggagcc
     2401 gtggccggac ttcatggcct tctacggcac ctccgccacc ggagccggct ggatcgtgcc
     2461 caagaagtac atcgagaagg tcggcgagga ggcctacaag agggctccgg tgggtgcggg
     2521 cccgttcaag ttcgtctcct tcaccccggg catcgagctg accctcgagg cgttcgggga
     2581 ctactggcgg aaggccccgc aggtgaagcg gctgatcatg cgctcgatcc ccgacgagac
     2641 cacccgcgcg gccgcggtga agaccggcga ggtggacgcg gcctacctgt tcgtcggccc
     2701 ggtggcggag gacctgaagc gctcgcccgg cgtccaggtg gtcgcgccgc tgctgtacgg
     2761 catctactgg ctcgacttcc tggaccagtg ggacccgaag tcgccgtggc acgatcggcg
     2821 ggtgcgccag gcggccagcc tcgtgatcga tcggaacgcg atcaaccagg cggagatgct
     2881 gggcctcggc aaggcggcgg gcgcgttcgt gccacccgag ttcgacttcg ccctgcgcgt
     2941 ggatccgccc gccttcgatc ccaagcgggc caagcagctc atggtcgagg ccggctatcc
     3001 gaacggcttc gacgcgggcg acctcacccc gctgccgccc tacaccaccg tggcggaggc
     3061 ggtcggcggc aacctgcagg cgatcgggat ccgcacgagc gtgcgcacga tggagcgcgc
     3121 caccttcctc accgcctggc gcgagaagaa gctgcgcggc ctgctgatcg gggccaccgg
     3181 cgccgccggc aacgcggcca cccgcctgga ggcgttcttc accaagggcg gcacctaccc
     3241 ctacggcacg cgcccggaga tcgacgatct cttccagcgc caggcggccg agaacgaccg
     3301 caagcagcgc gaggcactgc tccaccagat ccagaagatc gtcgccgacc agacgctggt
     3361 ggcgccgatc ttccagcagg cgttcctctg gggggtcggc tcgcgggtgg agcagcccgc
     3421 cgcgggcctc atccagggct acccgtacgc ggcgccctgc gaggatctga agctcaagta
     3481 agcctgcggg ccgctacgcg tcgcgcgggt cgtcgcggat gaagtgcccg ctgcgcaggt
     3541 cgcgcagcgc ctcctcgatc tccgcggtgg tgttcatgac gaacgggccg tggcgggcga
     3601 tcggctcgcc gagcggccgc gccgccgcga gcaggaaccg cccgccgccg ggcgcgcccg
     3661 ccgcctccac gaggtcgccg tcgccgaaga cgaccagcgc cggcgcgtgc gccgccttcc
     3721 ggtcggggcc gaagcgcacc tcgccctgat ccacgtacgc gaagacggtg tgcccccgcg
     3781 ggaccgtctc gcggaacgtc gcgccctccg gcaccgtcac gtcgaggaac ttgggcgcca
     3841 ccgcgaggcc ttccaccggc ccgatcagcc gcccgtccgc cgcgtcgccc gcgatgacgc
     3901 gcgcccgcgc gcccccgtcg agcgcgctct cggccaggtg gctcccggcc aggtcccggt
     3961 acttcggccg cgtcatcttc tcgcgcgcgg gcaggttgag ccagagctgg agccccccga
     4021 tgccctcggg ccgcacctgc ggcatctcct cgtggaggat gccgctgccg gaggtcatcc
     4081 actggatgtc gccgggcccg atgctcccgc ggtggccgag cgaatcgcga tgctggacct
     4141 ctcccgtgcg gacgaacgtc accgtctcga tgccgcggtg cggatgataa ggaaatccgg
     4201 gctcgtagtc ctcggggcgg accgactcga agtggtccag gagcaggaac gggtcgagat
     4261 ggtcgagccg ccgcgttccg atgctgcggc ggagatgcac cccggcgccc tccaccaccg
     4321 gctcgggcct gacgacctgg gcgaccgggc gtgaggtcgg gctcatcgcg aggcccctca
     4381 ggcgcgctcg aggatgacga ccgggatcgt ccgcgaggtc ttcgcctggt attcgtcgta
     4441 gggcggccag atcgcggcca tcgtcttcca gagcgcgggc cgctccgccg cggaggcggt
     4501 gcgggcgcgc gccttgaagc ggtccgccat cacctgcacc gtcacctccg ggtgcgcggc
     4561 gaggttctcg taccaggccg ggtgctgggg ggccccgccg cgcgaggcca ccaccacgta
     4621 gcgatcgccg tcgcgtccgt agatcagcgg ggtggtgcgg gcctggccgg agcgccgccc
     4681 ggtggtcgtc aacaggaggg tggtgactcc ctcccagacg tggccgtcct tcccgccgct
     4741 ctcgacgtac cggcgggcgt gctcttgctg ccagccgggc ggccggcttc ccgatgcggg
     4801 gcgtggcgtc gacatgaggg gcacctcccg ccgtcagtct gccacgacgc cgggcggcct
     4861 gccacccgac ggggatcgac ggtcacgcgg tcagcgagcc accagcaccg cgacgggcga
     4921 gcgcctgagc gcggcgtcca ccacgctccc gaggaggaag tggcgcaccg cgcccgcgcc
     4981 ccgcgcgccc agcaccagca ggtcggcgcg ctcggcccgg accgcggcga gcagctccgg
     5041 gagcggcggc ccggcgcgca ccacgccccg cgcgcgccag cccgccttcg ccagcgcgcc
     5101 gaccgcggcc tcgagctggc gccgggccat cgcccggcgg ctccgatcca tggccgcggc
     5161 ctgcgtctcg agctgggccc gcatccgcgc cggcacgaac ggcatcgacg gcagccgcac
     5221 cgcctcgatc acccgcacgc acgtcacccg cccgccgcgg ggcggcgcga ggcgcgccac
     5281 gagcgcgacc gctcgccgcg agtgcggcga gccgtccact cccacgacga tccggtgata
     5341 gccggacgcc accgggcgct cctaggcgcc gaggcgggcg cgcgcgtcca tcgcgatcgc
     5401 gccgtctggg ctcgtcatca gg
//
LOCUS       DATMCV010000442         6163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_159729, whole genome shotgun sequence.
ACCESSION   DATMCV010000442 DATMCV010000000
VERSION     DATMCV010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159729"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1120
                     /locus_tag="VNU03_10170"
     CDS             200..1120
                     /locus_tag="VNU03_10170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP74873.1"
                     /translation="MAIRNFVGPGEVPDIQGLYAYSERAEALGLESLWAWDHVLLGVE
                     PSFPIVDSITMLGAVAARTRAIKLGTGVLVLPLRNPVVAAKALGSLDVISGGRLVLGV
                     AAGWYAREFDAVGVPFKQRGRHFERNLDILMRLWTEERVTLKVDEFNLREAVMVPRTA
                     QRPRPPVLIGGYVDAVLKRAGTVGDGWLTYFYTPESFTRSWEKVKAFAREAGRDPAKL
                     TATNQLAVYVGRNREETTADMRKWLSTEWDTAAWSESTIDHAVHGSVAECIEQLRAHV
                     RTGVHRIILIPYRYQPEQVERIAKEVLPKL"
     gene            1117..1905
                     /locus_tag="VNU03_10175"
     CDS             1117..1905
                     /locus_tag="VNU03_10175"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HWP74874.1"
                     /translation="MSQPVYIDINCDMGESYGRWTLGHDEEVMPNITSANIACGFHGG
                     DPHVMRQTVALALQHGVAVGAHPGLPDLMGFGRRRMDVSPAELKDYHRYQVGALSAFA
                     QAAGTRVQHVKPHGIQYHMFEENLPLARATAEQVLELDPEMILMTMAMTKYDTEARKV
                     KGLRIAAEGFADRVYADDGQLVTRKLGKDALITDPAKAAEQAVRMAMEGKVRTITGKV
                     IDARVETICIHGDSPGSDKIVAAVREGLVKAGATVKPLRDWLPR"
     gene            2376..2717
                     /locus_tag="VNU03_10180"
     CDS             2376..2717
                     /locus_tag="VNU03_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74875.1"
                     /translation="MMLTERTSGNTTLRLLSGSGYRRESSAMPSDSQIAHALFDAQSA
                     PSVPSRKSFDEILFMGQHSSGWTQVFKQLKSEGLIQEQTLGHVVARWTNRTIRSRRPI
                     LAHAELEPASA"
     gene            complement(2736..3755)
                     /locus_tag="VNU03_10185"
     CDS             complement(2736..3755)
                     /locus_tag="VNU03_10185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP74876.1"
                     /translation="MKITFLCPHLRIAGGVRAILLHADRLARRGHEVTLLVPVKRTLA
                     AWWRNRVRTGPDWMPGLEARVRWVPEWSAARVPDGDVVIATSWQSVETVAAAPPRCGR
                     KFYFIQHYESLYHGEAAQVDATYRVPLRKIVISTWLADVMAERFETPAAVVVTPVEPG
                     LFHPVPAEEDGKLRVLMLFHEYPWKGVADGLEAFSRVRARHPELRLVGFGVKPPPRKL
                     PFAEFHENLPQERLAWLYSRCPIYLCPSWDEGLGMPAMEAMACGATLCTYDNGGCRDY
                     AIDGRTAVVAPRRDVDALAWGLSRLVEDADLRRRLARSGQDFVRTQFDWERATARLEA
                     LLAAR"
     gene            complement(3758..5410)
                     /locus_tag="VNU03_10190"
     CDS             complement(3758..5410)
                     /locus_tag="VNU03_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWP74877.1"
                     /translation="MADLNGGHLVARTLRQAGVGHVFTLCGGHILPIYDGCITEGVQV
                     VDMRHEQACAHAADAYARLTRNIGVALVTAGPGVTDAVTGIANAYSARSPVLLIGGAA
                     PLGLRGLGSLQEVEQVDLLRPITKGSWTVSETRQIPEVLTTAIRHALTGRPGPVFVEI
                     PVDLLMTTVEDRMAPIPTAYAHRRPTAPDEASVLELAALLARAERPMIMAGSGVYWDD
                     AAADLRGLAERAGVPVFTNGAGRGCLPHDHPEAFAQARGFALGQADLVVVLGTPLDFR
                     LGYGRAPSFAENVQVAMVDCDPVELGRNRPLAVGLTSHIGLTLRALADALPRDLAGRF
                     DRWRDAVRKKETDSQARLMAERLSDDVPISHYRLGHEIAAVVDAGTTVVGDGGDVVGC
                     ASKIVALSRPGQWLDPGPFGCLGVGPSFAIAAKLLRPEQRVLLIAGDGAFGLNGMEME
                     TAVRFKLPMTVVVGNDGGWGQIRNPQLSFFGAERSVATSLPFTRYDRMVEALGGKGIH
                     VTEPGQLRPALERALGSGEVYCINVVLDPAAYRRTGQVSMAI"
     gene            complement(5412..5828)
                     /locus_tag="VNU03_10195"
     CDS             complement(5412..5828)
                     /locus_tag="VNU03_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP74878.1"
                     /translation="MKAQVYHFQINVRDPARSLPFYKALLGYLEYRTVYESDTVAGFS
                     GSGADIWVIATEPAHAGHGFHRKRPGLNHVAFRVERREEVDRFRDEFMGPRRLPPLYA
                     TPREFPEYRPGYYAVFFEDPDRVKLEVVHIPGKPGG"
     gene            complement(5825..>6163)
                     /locus_tag="VNU03_10200"
     CDS             complement(5825..>6163)
                     /locus_tag="VNU03_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74879.1"
                     /translation="RSILTHLYETGYGRPTALIYWARNPGWLAYDPDFRSLARRYPGF
                     SYLPVLREPPATWRGEKGEPAEAVERLVYGIDRLIVYVCGGGETINRVRDVLVKKGMD
                     RKSVKWEKFW"
BASE COUNT          982 a         2205 c         2139 g          837 t
ORIGIN      
        1 cgaccgacga agagctcgcg atcctgcgca tgctcgaccc gaagcgccag ttcatcggat
       61 gaagcgtcga gacgtccacg tgaaaacggt tgtccgccac tgggggagtg cgggggccat
      121 ttctgggccc ccgcagatga agagccttaa agaagtaaac ggctagaacg gagggacaca
      181 cgcgttgaca gacttcggct tggcgattcg caacttcgtg ggccccggcg aggtgccgga
      241 catccagggc ctctacgcgt actcggagcg cgccgaggcc ctcggcctcg agtcgctctg
      301 ggcgtgggat cacgtgctcc tgggcgtgga gccctccttc cccatcgtgg actccatcac
      361 catgctgggc gcggtggccg cgcggacacg cgcgatcaag ctcggcaccg gggtcctggt
      421 gctgccgctg cgcaacccgg tggtggcggc caaggccctc ggcagcctcg atgtgatctc
      481 ggggggccgc ctcgtcctcg gggtcgcggc gggctggtac gcccgcgagt tcgacgcggt
      541 gggcgtgccc ttcaagcagc ggggccgcca cttcgagcgc aatctcgaca tcctcatgcg
      601 gctctggacc gaggagcggg tgaccctcaa ggtggacgag ttcaacctgc gcgaggcggt
      661 gatggtgccc cgcaccgcgc agcgcccgcg gccaccggtc ctgatcggcg gctacgtgga
      721 tgcggtgctc aagcgggccg gcacggtggg cgacgggtgg ctcacctact tctacacccc
      781 ggagagcttc accaggtcct gggagaaggt gaaggccttc gcacgcgagg cgggtcgcga
      841 ccccgcgaag ctcaccgcca ccaaccagct cgcggtgtac gtgggccgca accgggagga
      901 gaccaccgcg gacatgcgca agtggctctc caccgagtgg gacaccgcgg cctggagcga
      961 gtccaccatc gaccacgccg tccacggctc ggtcgccgag tgcatcgagc agctgcgcgc
     1021 ccacgtgcgg acgggcgtgc accggattat cctgatcccc taccgctatc agccggagca
     1081 ggtcgagcgc atcgccaagg aggtcttgcc caagctatga gccagcccgt ctacatcgac
     1141 atcaactgcg acatgggaga gagctacggc cgctggaccc tgggccacga cgaggaggtc
     1201 atgcccaaca tcacctcggc caacatcgcc tgcggcttcc acgggggcga cccgcacgtg
     1261 atgcgccaga cggtggccct ggcgctgcag cacggcgtgg cggtgggcgc ccaccccggc
     1321 ctgcccgacc tgatgggctt cggccggcgc cgcatggacg tctcgccggc cgagctgaag
     1381 gactaccacc gctaccaggt cggggcgctg tccgccttcg cccaggccgc cggcacccgc
     1441 gtgcagcacg tcaagccgca cggcatccag taccacatgt tcgaggagaa cctgccgctc
     1501 gcgcgcgcga ccgccgagca ggtgctcgag ctcgatcccg agatgatcct gatgaccatg
     1561 gccatgacca agtacgacac cgaggcccgg aaggtgaagg gcctgcgcat cgcggccgag
     1621 ggcttcgcgg accgggtcta cgccgacgac ggccagctgg tgacgcgcaa gctcggcaag
     1681 gacgccctga tcaccgaccc ggccaaggcc gccgagcagg cggtgcgcat ggcgatggag
     1741 ggcaaggtgc gcacgatcac cggaaaagtg atcgacgcgc gcgtcgagac gatctgcatt
     1801 cacggcgaca gccccggctc ggacaagatc gtggccgcgg tgcgcgaggg cctggtcaag
     1861 gcgggcgcga cggtgaagcc gttacgcgac tggttgccgc gctgagcggc ggttcaccgt
     1921 agcgcaggtc ctgcggggtc ggaccgaggc gtccgaaatg ggtacagtgt ctggcgctcc
     1981 tcgtcaccgg gcaccggagc tcggtccgac ccaccgctcg taactgttcg acactccagc
     2041 tctttcctcc cctccgaaaa ctggcaccga gtttgccgta gagaggctcg gcacatagcc
     2101 atggcggact acacggacgg caggagcgtg aagggcgcga agaccggcgg cacccggccc
     2161 ggcgaaatca agccgagctc ggggcatcgc ggccatcgtc tcgcgggact tcacgcgcct
     2221 cgcggggact tccggcagcg tcacgccctg gagtcccgac cgcacgagta tcccggtcag
     2281 ctcgcgcggg ctcatcgcct cccgcatcat ccgcgaggcc taggtcgatg agtgcaaccg
     2341 atccggccgg aatcgccaca ggggaggaac ggaccatgat gctcacagag cgcaccagcg
     2401 gcaacacgac gcttcggctg ctctccgggt cgggttaccg gcgagagagc agcgccatgc
     2461 cctcggactc gcagatcgcc cacgccctct tcgacgcgca gagcgctccg agcgtgccgt
     2521 cgcggaagag cttcgacgag atcctgttca tggggcagca cagctcgggc tggacgcagg
     2581 tgttcaagca gctgaagtcg gaaggtctga tccaggagca gacgctgggg cacgtggtgg
     2641 cgcggtggac caaccgcacg atccgcagcc gccggccgat cctggctcac gccgagctcg
     2701 agcccgcttc cgcctagtcc gtttcgtttc gagcgctacc gcgcggccag caacgcctcg
     2761 agccgcgcgg tagcgcgctc ccagtcgaac tgggtcctca cgaagtcctg cccgctccgg
     2821 gccagccgcc gccgcaagtc agcatcctcc accagcctcg acagccccca cgcgagcgcg
     2881 tccacgtccc gccggggagc caccaccgcg gtgcgcccgt cgatggcgta gtcgcgacag
     2941 ccgccgttgt cgtaggtgca gagcgtcgcg ccgcaggcca tcgcctccat ggcgggcatg
     3001 ccgagcccct cgtcccagga cgggcagaga tagatcgggc agcggctgta gagccaggcc
     3061 aggcgctcct ggggcaggtt ctcgtggaac tcggcgaacg ggagcttgcg cgggggcggc
     3121 ttgaccccga agcccaccag ccgcagctcg gggtgccgcg ctcggacccg gctgaaggcc
     3181 tcgagcccgt cggccacccc cttccaggga tactcgtgga agagcatcag cacccggagc
     3241 ttgccgtcct cctcggcggg aaccggatgg aagagacccg gctccaccgg ggtgaccacc
     3301 accgcagccg gcgtctcgaa ccgctcggcc atcacgtcgg cgagccacgt cgagatcacg
     3361 atcttccgca ggggcacgcg gtaggtcgcg tccacctggg ccgcctcgcc gtggtagaga
     3421 ctctcgtagt gctggatgaa gtagaacttc cgcccgcagc gcggcggggc cgccgccacc
     3481 gtctccaccg actgccagga cgtggcgatc accacatcgc catccggcac acgggccgcg
     3541 ctccactcgg gcacccaccg cacgcgcgcc tcgaggcccg gcatccagtc cggcccggtc
     3601 cgaacccggt tgcgccacca cgcggcgagg gtgcgcttca ccggcacgag cagcgtcacc
     3661 tcgtggcccc ggcgggcgag gcggtcggcg tggagcagga tcgcgcgcac cccgcccgcg
     3721 atgcggagat gcgggcagag gaacgtgatc ttcatggtca gatggccatc gacacctgac
     3781 ccgtgcggcg ataggcggcc ggatccagca ccacgttgat gcagtagacc tcacccgagc
     3841 cgagcgcccg ctccagcgcg ggccggagct ggcccggctc ggtcacgtgg atccccttcc
     3901 cgcccagcgc ctccaccatg cggtcgtagc gggtgaacgg cagcgaggtc gccaccgagc
     3961 gctcggcccc gaagaacgac agctgcgggt tgcggatctg cccccacccg ccgtcgttgc
     4021 cgaccacgac cgtcatcggc agcttgaagc gcaccgcggt ctccatctcc atcccgttga
     4081 gcccgaaggc tccgtccccc gcgatcagga ggacgcgctg ctctggacgc agcagcttgg
     4141 ccgcgatggc gaacgacggg cccaccccga ggcagccgaa ggggcccgga tcgagccact
     4201 ggccgggccg ggagagggcc acgatcttcg acgcgcatcc gaccacgtcg ccgccgtcgc
     4261 ccaccacggt ggtcccggcg tccaccaccg ccgcgatctc gtggccgagg cgatagtggc
     4321 tgatcggcac gtcgtcggac aaccgctccg ccatgaggcg cgcctgcgag tcggtctcct
     4381 tcttccgcac cgcgtcccgc cagcggtcga agcgcccggc caggtcccgc ggcagggcgt
     4441 cggcgagcgc gcggagcgtg agaccgatgt ggctggtcag gccgaccgcc agcgggcggt
     4501 tgcgccccag ctccaccgga tcgcagtcca ccatcgccac ctggacgttc tcggcgaacg
     4561 agggcgcccg gccatagccc aggcggaagt cgagcggggt ccccagcacc accacgaggt
     4621 cggcctggcc cagcgcgaag ccgcgggcct gggcgaacgc ctctgggtgg tcgtgcggga
     4681 ggcagccccg ccccgccccg ttcgtgaaca ccggcacccc ggcgcgctcg gccagccccc
     4741 gcagatccgc ggccgcgtcg tcccagtaga cgccgctgcc cgccatgatc atcggtcgct
     4801 ccgcacgggc cagcagcgcg gccagctcca ggaccgacgc ctcatcgggg gcggtgggcc
     4861 gccggtgagc gtaggcggtc gggatcggcg ccatccgatc ctcgaccgtg gtcatcaaca
     4921 ggtcgaccgg gatctcgacg aagaccggcc ccggccggcc ggtgagcgcg tgccggatgg
     4981 cggtggtgag cacctcgggg atctggcgcg tctcggagac cgtccacgag cccttggtga
     5041 tcggccgcag gaggtcgacc tgctcgacct cttggagcga gcccaggccg cgcagcccga
     5101 gcggcgccgc gcccccgatg aggagcaccg ggctgcgcgc ggagtaggca ttggcgatgc
     5161 cggtcaccgc gtcggtgacg ccgggtccgg cggtgaccag ggccacgccg atgttgcggg
     5221 tgagccgcgc gtaggcgtcg gcggcgtggg cgcaggcctg ctcgtggcgc atgtccacca
     5281 cctgcacgcc ctcggtgatg cagccgtcgt agatcggcag gatgtggccg ccgcagagag
     5341 tgaacacgtg acccacgccc gcctggcgga gggtgcgcgc gaccaggtgg cccccattga
     5401 gatccgccac gctagccgcc cggcttgccg gggatgtgca ccacctcgag cttcacccgg
     5461 tccgggtcct cgaagaagac cgcgtagtag cccggccggt actccgggaa ctcccgtggc
     5521 gtcgcgtaga gcggcgggag ccggcgcggg cccatgaact cgtcgcggaa tcgatccacc
     5581 tcctcgcggc gctccacccg gaaggccacg tgattgaggc ccggacgctt gcggtggaac
     5641 ccgtgcccgg cgtgggccgg ctcggtcgcg atcacccaga tgtcggcccc gctccccgag
     5701 aagccggcga ccgtgtcgct ctcgtagacg gtgcgatact cgaggtagcc gaggagggcc
     5761 ttgtagaagg ggagcgagcg ggcgggatcg cggacgttga tctggaagtg atagacctgg
     5821 gccttcacca gaacttctcc cacttcacgc tcttgcgatc catgcccttc ttcacgagca
     5881 cgtcccgcac ccggttgatg gtctccccgc ccccgcagac gtagacgatc aaccggtcga
     5941 tgccgtatac gaggcgctcc accgcctccg cgggctcccc cttctcgccg cgccaggtgg
     6001 cgggcggctc gcgcagcacc ggcaggtagg agaaccccgg atagcggcgc gcgagcgagc
     6061 ggaagtcggg gtcgtaggcg agccagcccg ggttgcgggc ccagtagatc agcgcggtgg
     6121 gccggccgta gccggtctcg tagagatggg tcaggatcga gcg
//
LOCUS       DATMCV010000443         9024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_686227, whole genome shotgun sequence.
ACCESSION   DATMCV010000443 DATMCV010000000
VERSION     DATMCV010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686227"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VNU03_10205"
     CDS             <1..955
                     /locus_tag="VNU03_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP74880.1"
                     /translation="FVDRRIFRMGIVISALMVLLAVLFLLAARHYVALKDYSSLSYSR
                     VARRRLTAGRQALVVAFLSLLLLLSFIPYIGVTLASVGKGWALTPLPVAYTLQYYERV
                     IVETPKYILNSFLYSGLAVVVCVLVGVPIAWLLARSTVPGRGALDAVNTLILAVPGTA
                     LGIAYIRAFHFDLPGFDRGLTSFWIVMPLVLAVRRLPYTVRGSFASLLLVHRSLEEAA
                     GSVGASGPRTFRDVTLPLIWKGILVGGLFSFMTSLQEASAVLFLALGGWESITNGIFA
                     FYIAGSANEAAALGVILIVVAAISVAVINRMAGTRMGGVFG"
     gene            1075..1311
                     /locus_tag="VNU03_10210"
     CDS             1075..1311
                     /locus_tag="VNU03_10210"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HWP74881.1"
                     /translation="MAPEERGPSQGQTAQLVNLGTLLFACVAVGLAAGYFADRWLGTS
                     PWLLLAGLTFGIVAAAVNFYRTIKRLNEQDRDDR"
     gene            1301..1669
                     /locus_tag="VNU03_10215"
     CDS             1301..1669
                     /locus_tag="VNU03_10215"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HWP74882.1"
                     /translation="MIADLAARVTWTGGVTVAALTAGAWWLAGPPAAAGVGGAGSIAL
                     LNFRWLARDVSRATALVAEGGGGVGRIGRIGLRQLVTFGALGLLIAQGWAHPVGVGVG
                     LAALPPVLLIQGLLDAREHA"
     gene            1673..2428
                     /gene="atpB"
                     /locus_tag="VNU03_10220"
     CDS             1673..2428
                     /gene="atpB"
                     /locus_tag="VNU03_10220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWP74883.1"
                     /translation="MELIEHPPILHIPAIPDHILYTWIVMAILVVVSFVATRRVSLVP
                     TGTQNFMELVLDQFVKELDETIGHEGRPYLPLIATLGLFILASNLMGLVPGLVAPTAN
                     LNTTAACAIIVFITYHVIGVRKQGFIPYMKHFAGPVPAVLKPLMFVIEIISHCARPLS
                     LSLRLFGNMMAGHILLAIFFFMMGFDGMLGWALSGTLGGIVVGAPAAVIMVVFVVGFL
                     LPLKILVAFIQTFIFCKLAMLYIAAAVEEAEHH"
     gene            2459..2776
                     /locus_tag="VNU03_10225"
     CDS             2459..2776
                     /locus_tag="VNU03_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HWP74884.1"
                     /translation="MQRILTVSVMAILAVPGMAWAADAAGGSDMAKAFAVLAAGLGFG
                     LAAGLCGLGQGRAAGSAVDAMARQPGAIARIQTAMLLGLAFIESLVLFVFVIAVILLF
                     VKS"
     gene            2884..3534
                     /locus_tag="VNU03_10230"
     CDS             2884..3534
                     /locus_tag="VNU03_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HWP74885.1"
                     /translation="MARRSPFKIPRIPEMTVRRLSVYTRSLLQLEEDGVKTISSQELA
                     ERFSLNSAQVRKDLAYFGEFGVRGIGYYVSGLKAELQRILGLDREWQVALVGFGNLGS
                     ALFHYKGFARQGFRISTIFDDDPAKWGKAVDSVTIQPMQDLPKEAKARNLQIAIVAVP
                     GESAQAVTEKLVAAGIKTILNFAPGRIKAGRDVRLKNVDLAIELETLSFYLAQGSR"
     gene            3596..6847
                     /gene="mfd"
                     /locus_tag="VNU03_10235"
     CDS             3596..6847
                     /gene="mfd"
                     /locus_tag="VNU03_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWP74886.1"
                     /translation="MSVSAQSVLSRWAAFDEVLAALSKGVPCLRADGLWGSSRALATA
                     GLLQRSGRPILLLAPGPVQRHQAAQDAAFFLATLSGGPVDGPVGEGRVLEFPSGSGAS
                     WRGGRHREPDAERALCCRRLLDGDAVAIVATPSGLAIPVPPPESFKRRTFTLTVGEGS
                     DREILLALLEAAGYERVETVLEVGQWSLRGGIVDIFSPTHAQPVRAEFFGDEVESLRL
                     FDPTSQRSIGQLRELTVLPLGSPDAAPVTLLDYLPAETLIVLEDPALLEAPPDDAPSA
                     APLATLLARFQRLELPLLQRGDAAVPRVSMGARSVGGFRGQFKTLATEIRDWRGEGFM
                     VRLVVDDERQRDRLRQMLAEHDLEPWPEATLWSSEGLGVVVGECSAGFQIPALGLVVL
                     CEEEIFGAQRRRLRRPLFQRGAAIASFNDLAPNDLVVHEQHGIARYHGLRTLSTDGHD
                     ADFLLLEYAEGGRLYLPVERLDLISKYMGAPEGAARLDRLGGGAWQRLKESVRAALRE
                     MAEELLKLYATRSVAERPRFADDTPWQGEFEASFRFEETPDQMRAIEEVKADMVGARP
                     MDRLVAGDVGYGKTEVALRAAFRAVADGRQVAVLVPTTVLAQQHYNTFVERFAPFPAK
                     VELLSRFRSPKEQKEVVAGLATGTVDVVIGTHRILSKDVQFKDLGLLVVDEEHRFGVT
                     HKERLKQLRTSVDVLTLTATPIPRTLHMALSGVRDLSVIETPPLDRLPVETVVTAFNR
                     TVIREAIERELNRGGQVFFVHNRIQSLGSMTHFIQTLAPEARVVMGHGQMAERELESV
                     MVKFVDGQADVLVSTAIVESGLDIPASNTIIINRADRFGLAQLYQLRGRVGRERQQAY
                     AYLLVPADGRIDETAQKRLRVIEESTELGAGLRLAMRDLEIRGTGNLLGAAQHGHIAA
                     VGFDLYSKLLAEAVRELKGEPAAERVDPVISADVEALLPESFVPEVNQRLALYKRLAE
                     IERPEEVVDARAELADRYGPLPPAVEGLLDVIGLRVAARELGVERVEAGGGRAILTFA
                     PSTKVTPERMLKAIAASRGGLALKKEYTVEARIPTQPWPAVRDALVKLLDSLR"
     gene            6844..7941
                     /locus_tag="VNU03_10240"
     CDS             6844..7941
                     /locus_tag="VNU03_10240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWP74887.1"
                     /translation="MRRRAGRRRPTVCLLAVLSLATAGCSMAIPSWVPLLGSAPKAPT
                     AAEAPKPSGSAPILTSRDQIESSPDVVDRVICVVNNDAITQYELDEAELYYLAETREQ
                     MTDGEARKALRARLLQTLIENRIQLQQAEREKVVIEDAELAENVADIMKKLKAKDDKE
                     FEEIIKSQGLTIEGVRKRLREQLMVQRVIRRKVALRISVTEQEIDKYLVDNREKLETG
                     LTFAPRHILVVPDPNKGEEGWLEARKKAEDVYALLLEGQDFGELAKKFSEDPSGKDGG
                     GLGNIKRGELAPDIEEAIVRLQPGEVSTPFRSQVGYHLFKLDSRETLSGDSLVQVRSQ
                     VRDILYRQKYDARLKDWLVEIKQRAIIDVRM"
     gene            8212..>9024
                     /gene="rho"
                     /locus_tag="VNU03_10245"
     CDS             8212..>9024
                     /gene="rho"
                     /locus_tag="VNU03_10245"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWP74888.1"
                     /translation="MNLSELKDKTIADLNNVAKELGVQGTSGLRKQELIFKILQGQAE
                     RDGLIFAEGVLEVLPDGFGFLRAPDYNYLPGPDDIYVSPSQIRRFDLRTGDTISGQVR
                     PPKDSERYFALLKVEAINFETPEQARDKTLFDNLTPLYPMERIRLEHDSDNLTTRAMD
                     LLCPIGKGQRGLIVAAPRTGKTMLLQSLANSITTNHPEVTLIVLLIDERPEEVTDMQR
                     SVRGEVISSTFDEPASRHVQVAEMVIEKAKRLVENKRDVVILLDSITRLARAY"
BASE COUNT         1322 a         3127 c         3143 g         1432 t
ORIGIN      
        1 gttcgtcgac cggcgcatct tccgcatggg tatcgtgatc tccgcgctga tggtgctgct
       61 cgcggtcctc ttcctgctcg cggcgcggca ctacgtggcg ctcaaggact actcgtcgct
      121 ctcctacagc cgggtcgccc ggcggcggct caccgcgggc cggcaggcgc tggtggtggc
      181 cttcctgtcg ctcctgctgc tgctgtcctt catcccctac atcggggtga cgctcgcctc
      241 ggtggggaaa ggctgggcgc tcaccccgct gccggtggcc tacacgctgc agtactacga
      301 gcgcgtgatc gtggagacgc ccaagtacat cctcaacagc ttcctctact ccgggctcgc
      361 ggtggtggtc tgcgtgctgg tcggcgtgcc gatcgcctgg ctgctcgcgc ggtccaccgt
      421 gccggggcgc ggggcgctcg acgcggtcaa caccctgatc ctcgccgtgc ccggcaccgc
      481 tctcggcatc gcctacatcc gcgcgttcca cttcgacctg cccggcttcg atcgcgggct
      541 cacctcgttc tggatcgtca tgcccctggt gctcgccgtg cggcggctgc cctacacggt
      601 gcgcggctcg ttcgcctcgc tcctgctggt gcaccgctcg ctcgaggagg ccgccggcag
      661 cgtcggggcc tccggcccgc gtaccttccg cgacgtgacg ctgccgctca tctggaaggg
      721 catcctggtc ggcggcctct tctcgttcat gacctcgctg caggaggcct ccgcggtgct
      781 cttcctggcc ttgggcggct gggagtcgat caccaacggc atcttcgcct tctacatcgc
      841 gggcagcgcc aacgaggcgg ccgccctcgg cgtgatcttg atcgtggtcg ccgccatcag
      901 cgtcgccgtc atcaatcgca tggccggcac ccgcatgggc ggcgtcttcg gctagcaggg
      961 ctgcagcctg ccttgtacct gggtggccgg tgatcttggg atctttaggt cgtgaaatca
     1021 atgagttgag ctcgcttgtc agcttccggg gcgtgtgata gtgttcctgt tcacatggct
     1081 ccggaagaac gggggccgtc gcaaggccag accgctcagc tcgtcaatct cgggacgctg
     1141 ctgttcgcgt gtgtcgcggt cggcctcgcg gcaggctact tcgccgaccg gtggctcggg
     1201 acgagcccgt ggctcttgct ggccgggctc accttcggca tcgtggcggc ggcggtgaac
     1261 ttctaccgga cgatcaagag actgaacgag caggatcggg atgatcgctg acctcgcagc
     1321 acgcgtgacc tggaccggag gcgtcacggt ggccgccctg acggcgggcg cctggtggct
     1381 ggccggcccg cccgcggcgg ccggagtcgg cggcgccggc tccatcgcgc tgctgaactt
     1441 ccgctggctc gcccgcgacg tgagccgcgc gaccgcgctg gtcgcggagg gcgggggcgg
     1501 ggtggggcgc atcggccgca tcggtctccg gcagctcgtg accttcggcg ccctgggcct
     1561 gctgatcgcg cagggctggg cccacccggt cggggtcggc gtcgggctgg ccgccctgcc
     1621 gccggtgctg ctgatccagg gtcttctcga cgccagggag cacgcctgat ccatggagct
     1681 gatcgagcac ccgccgatcc tgcacatccc ggccatcccg gaccacatcc tctacacgtg
     1741 gatcgtgatg gcgatcctgg tggtggtctc cttcgtggcc acgcgccgcg tctccctggt
     1801 gcccaccggc acccagaact tcatggagct ggtgctcgac cagttcgtga aggagctcga
     1861 cgagacgatc ggtcacgagg gccggcccta cctgccgctg atcgccaccc tcgggctgtt
     1921 catcctcgcc tcgaacctca tggggctggt cccggggctg gtggcgccga ccgccaacct
     1981 caacaccacc gcggcgtgcg cgatcatcgt gttcatcacc taccacgtga tcggggtgcg
     2041 caagcagggc ttcatcccct acatgaagca cttcgcgggg ccggtgcccg cggtgctcaa
     2101 gccgctgatg ttcgtcatcg agatcatcag ccactgcgcg cggccgctgt cgctgtccct
     2161 ccgcctcttc ggcaacatga tggccggcca catcctgctc gccatcttct tcttcatgat
     2221 gggcttcgac gggatgctgg gctgggcgct ctccggcacc ctcggaggga tcgtggtggg
     2281 agccccggcc gcggtcatca tggtcgtgtt cgtcgtgggc ttcctgctcc cgttgaagat
     2341 cctggttgcg ttcatccaga ctttcatctt ctgcaagctg gccatgctct acatcgcggc
     2401 ggcggtcgag gaagccgagc accactagtc gcagtcaccc tcaacaagga ggaaagccgt
     2461 gcaacgcatt ctcaccgtga gtgttatggc gattctggcg gtcccgggga tggcctgggc
     2521 ggccgatgcc gccggcgggt cggacatggc gaaggccttc gcggtcctgg ccgccggtct
     2581 cggcttcggc ctggccgcgg gtctctgcgg cctgggtcag ggccgcgcgg cgggctcggc
     2641 ggtggacgcc atggcgcggc agcccggcgc gatcgcccgg atccagaccg cgatgctgct
     2701 cggtctggcg ttcatcgagt cgctcgtgct gttcgtgttc gtcatcgcgg tcatcctgct
     2761 gttcgtgaag tcctagggag aagctcgtag gcaccgtccc ggggcccagg ccccgggacg
     2821 gcacgcgggc ccgcccgcga aaggaatgcc ccacccggcg cgagcagcgc cgccgagacg
     2881 aacatggcac gacggtcacc gttcaagatc ccccggatcc cggagatgac ggtgcgccgc
     2941 ctgtccgtct acacccgcag cctcctgcag ctcgaagagg acggggtgaa gacgatctcg
     3001 tcgcaggagc tggccgagcg gttcagcctc aactcggccc aggtccggaa ggatctcgcc
     3061 tacttcggcg agttcggggt gcggggcatc ggctactacg tctccgggct caaggccgag
     3121 ctgcagcgca tcctgggcct cgaccgcgag tggcaggtgg cgctggtggg tttcggcaac
     3181 ctgggctcgg ccctcttcca ctacaagggc ttcgcgcgcc agggattccg gatctccacg
     3241 atcttcgacg acgaccccgc caagtggggc aaggcggtcg acagcgtgac gatccagccg
     3301 atgcaggacc tgccgaagga ggccaaggcc cggaatctgc agatcgcgat cgtggcggtg
     3361 ccgggggagt ccgcccaggc ggtgaccgag aagctggtgg cggccggcat caagaccatc
     3421 ctcaacttcg cgccggggcg catcaaggcg ggccgggacg tccgcctgaa gaacgtggac
     3481 ctggccatcg agctcgagac cctgtccttc taccttgctc aaggctctcg ctgagggccc
     3541 cgcggcgttg acgccgcgcg cccccggtgg tacctctgat cagtcgttgc gtgtgatgag
     3601 cgtgtccgcc cagtccgtcc tgtcccgctg ggccgccttc gacgaggtcc tggcggccct
     3661 ctccaaggga gtaccctgcc tgcgcgccga cggcctctgg ggctcgtccc gcgccctggc
     3721 gaccgccggg ctgctccagc ggtccgggcg gccgatcctg ctccttgccc cgggcccggt
     3781 ccagcgccac caggccgccc aggacgccgc gttcttcctg gcgaccctct ccggtggccc
     3841 ggtggacggg ccggtcggcg aggggcgcgt cctcgagttc ccgtcgggct cgggcgcctc
     3901 ctggcgcggc gggcgccacc gcgagccgga cgccgagcgt gcgctctgtt gccgccggct
     3961 gctggacggc gacgcggtgg ccatcgtggc gacgccgtcc gggctcgcga tcccggtgcc
     4021 gccgcccgag tcgttcaagc gccgcacgtt caccctgacc gtcggcgagg gcagcgaccg
     4081 ggagatcctg ctcgcgctcc tcgaggcggc cggctacgag cgggtggaga cggtgctcga
     4141 ggtcgggcag tggagcctgc ggggcggcat cgtcgacatc ttctcgccca cccacgccca
     4201 gccggtgcgc gccgagttct tcggcgacga ggtggagtcg ttgcgcctct tcgatcccac
     4261 ctcgcagcgc tcgatcgggc agctccggga gctgaccgtg ctgccgctcg gctctccgga
     4321 cgcggcgccg gtcaccctgc tcgactacct ccccgccgag accctgatcg tcctggagga
     4381 tcccgcgctg ctggaggcgc cgcccgacga cgccccgtcg gcggccccgc tcgcgaccct
     4441 gctcgcgcgc ttccagcggc tcgagctgcc gctgctccag cggggagacg cggcggtgcc
     4501 ccgcgtctcc atgggcgcgc gctcggtggg cggcttccgc ggccagttca agacgctcgc
     4561 gaccgagatc cgcgactggc gcggcgaggg cttcatggtg cgcctcgtcg tggacgatga
     4621 gcggcagcgg gatcgcctgc gccagatgct ggccgagcac gacctcgagc cgtggccgga
     4681 ggccaccctc tggagctcgg agggcctcgg ggtggtggtg ggcgagtgct cggccggatt
     4741 ccagatcccc gcgctcggcc tggtcgtgct ctgcgaggag gagatcttcg gggcccagcg
     4801 gcgaaggctg aggcggccgc tgttccagcg gggcgcggcc atcgcctcgt tcaacgacct
     4861 ggccccgaac gacctggtgg tgcacgagca gcacggcatc gcgcgctacc acggtctgcg
     4921 cacgctctcc accgacggcc acgacgccga cttcctgctg ctggagtacg ccgagggcgg
     4981 gcgcctctac ctgccggtgg agcggctcga cctcatctcg aagtacatgg gcgcgcccga
     5041 gggggcggcg cgcctggacc ggctgggcgg cggcgcctgg cagcgtctca aggagtcggt
     5101 gcgggccgcg ctgcgcgaga tggccgagga gctgctcaag ctctacgcga ctcgctcggt
     5161 ggccgagcgg ccgcgcttcg ccgacgacac cccctggcag ggggagttcg aggcctcgtt
     5221 ccgcttcgag gagacgccgg accagatgcg cgccatcgag gaggtcaagg ccgacatggt
     5281 gggggcgcgg ccgatggacc gcctggtcgc gggcgacgtg ggctacggca agaccgaggt
     5341 ggcgctgcgc gcggccttcc gggcggtggc ggacggccgg caggtggcgg tgctcgtgcc
     5401 gaccaccgtg ctcgcccagc agcactacaa caccttcgtc gagcgcttcg cgccgttccc
     5461 ggccaaggtc gagctgctct cccgcttccg tagcccgaag gagcagaagg aggtggtagc
     5521 cgggctcgcc accggcacgg tggacgtggt gatcggtacc caccggatcc tctcgaagga
     5581 cgtgcagttc aaggacctgg gcctgctcgt ggtggacgag gagcaccgct tcggggtcac
     5641 ccacaaggag cggctcaagc agctgcgcac ctcggtggac gtgctcacgc tcaccgcgac
     5701 cccgatcccg cgcaccctcc acatggcgct gtccggagtg cgcgacctct cggtcatcga
     5761 gaccccgccg ctcgaccggc tgccggtgga gacggtggtg accgcgttca accgcaccgt
     5821 gatccgcgag gcgatcgagc gggagctgaa ccggggcggg caggtgttct tcgtgcacaa
     5881 ccgcatccag tcgctcggct ccatgaccca cttcatccag accctggccc cggaggcgcg
     5941 ggtggtgatg ggccacggcc agatggcgga gcgggagctc gagagcgtga tggtgaagtt
     6001 cgtggacggc caggccgacg tgctggtctc gaccgccatc gtggagtcgg gcctcgacat
     6061 cccggcctcc aacaccatca tcatcaaccg ggccgaccgg ttcgggctgg cccagctcta
     6121 ccagctccgg ggccgggtgg gccgggagcg gcagcaggcc tacgcctacc tcctggtgcc
     6181 cgccgacggc cggatcgacg agacggccca gaagcgcctg cgggtcatcg aggagtccac
     6241 cgagctgggg gcgggcctcc ggctggccat gcgcgacctc gagatccggg gtaccggcaa
     6301 cctcctgggg gcggcccagc acgggcacat cgcggcggtg gggttcgatc tttacagcaa
     6361 gctcctggcg gaggccgtgc gagaattgaa gggcgaaccg gcggccgagc gcgtcgaccc
     6421 ggtgatctcg gccgacgtgg aagcgctcct gcccgaatcg ttcgtccccg aggtgaacca
     6481 gcgactcgcg ctctacaagc ggctggccga gatcgagcgt cccgaggaag tcgtcgacgc
     6541 gcgcgccgag ctggccgacc gctacgggcc gctgccgccg gcggtcgagg ggctgctgga
     6601 cgtgatcggg ctccgggtgg ccgcccgcga gctcggggtg gagcgggtgg aggccggggg
     6661 tgggcgggcg atcctcacct tcgcgccgtc gacgaaggtg accccggaga gaatgctcaa
     6721 ggcgatcgcg gccagccgag gaggcctcgc gttgaagaag gagtacacgg tggaagcgcg
     6781 gattccgacc cagccctggc ccgcggtgcg cgacgctctc gtgaagctcc tggactcgct
     6841 gcggtgaggc ggcgcgccgg ccggcgtcgc ccgaccgtct gtctcctcgc cgtcctgtcc
     6901 ctggcgacgg ccggctgctc gatggcgatc ccgagctggg tcccgctgct cgggagcgcc
     6961 cccaaggccc ccaccgccgc ggaggcgccc aagccctcgg ggtcggcgcc gatcctgacc
     7021 tcgcgcgacc agatcgagtc ctcgccggac gtggtcgacc gggtcatctg cgtggtgaac
     7081 aacgacgcga tcacccagta cgagctggac gaggccgagc tctactacct ggccgaaacc
     7141 cgcgagcaga tgacggacgg ggaggcccgc aaggcgctgc gcgcccgcct gctccagacc
     7201 ctgatcgaga accgcatcca gctccagcag gccgagcggg aaaaggtcgt gatcgaggac
     7261 gcagagctgg ccgagaacgt cgccgacatc atgaagaagc tcaaggccaa ggacgacaag
     7321 gagttcgagg agatcatcaa gtcccagggc ctcaccatcg agggagtccg gaagcggctc
     7381 cgcgagcagc tcatggtcca gcgggtgatc cgccggaagg tcgccctgcg catctcggtc
     7441 accgagcagg agatcgacaa gtacctggtc gacaaccggg agaagctcga gaccgggctc
     7501 accttcgcgc cgcgccacat cctggtggtg cccgatccga acaagggcga ggaggggtgg
     7561 ctcgaggcgc gcaagaaggc ggaggacgtc tacgcgctct tgctggaggg gcaggacttc
     7621 ggcgagctgg ccaagaagtt ctccgaggac ccgtccggca aggacggcgg cggtctcggc
     7681 aacatcaagc gcggggagct ggcgcccgat atcgaggaag cgatcgtgcg cctgcagccc
     7741 ggcgaggtct ccactccctt ccgctcccag gtgggctacc acctcttcaa gctcgactcg
     7801 cgcgagacgc tgagtggcga ctccctggtc caggtgcgga gccaggtccg cgacatcctc
     7861 taccgccaga agtacgacgc gcgactcaag gattggctgg tcgagatcaa gcagcgggcg
     7921 atcatcgatg ttcggatgta gcgggcggac gtaggcgggc ggaagtagac gctgggccgg
     7981 ggaaaatgag agttgacagc ccgggaaagc gtgtgggaca gtgatccgcg tagcacggca
     8041 cccaacctcc ttacagagta gacctcaaca cacgcaaccc gccggagaac gaccatctcc
     8101 tcatcagagc gcagcccgaa gcgtccctcg tcgcccaagc cgccgtcgcc gtctgtcccc
     8161 gagcccagtc ccaccgaagc gagcacggtg gtcggtgcag gaggaaagcg gatgaatctg
     8221 tccgagctca aggacaagac gatcgccgat ctgaacaacg tcgccaagga gctcggagtc
     8281 cagggcacca gcggcctccg caagcaggag ctgatcttca agatcctgca ggggcaggcc
     8341 gagcgtgacg ggctcatctt cgcggagggc gtgctcgagg tgctgccgga cggcttcggc
     8401 ttcctgcggg cgcccgacta caactacctg ccgggccccg acgacatcta cgtctcgccc
     8461 tcgcagatcc ggcgcttcga cctgcgcacc ggcgacacca tctccggcca ggtgcggccc
     8521 cccaaggaca gcgagcgcta cttcgccctc ctgaaggtgg aggcgatcaa cttcgagacg
     8581 ccggagcagg cgcgcgacaa gaccctcttc gacaacctga ccccgctcta cccgatggag
     8641 cggatccggc tcgagcacga ctcggacaac ctgaccacgc gggcgatgga tctgctgtgc
     8701 cccatcggca agggccagcg cggcctgatc gtggccgcgc cccgtaccgg caaaaccatg
     8761 ctgctgcaga gcctcgccaa ctcgatcaca accaatcatc ccgaggtgac gctgatcgtc
     8821 ctgctgattg acgagcgccc ggaagaagtc accgacatgc agcgctcggt gcgcggcgag
     8881 gtgatcagct ccacctttga cgagccggca tcccggcacg tgcaggtagc cgaaatggtg
     8941 atcgaaaaag ccaagcgcct ggtggaaaac aagcgcgacg tggtgatcct gctggattcc
     9001 atcacgcgtc tggcgcgcgc ctac
//
LOCUS       DATMCV010000444         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_686228, whole genome shotgun sequence.
ACCESSION   DATMCV010000444 DATMCV010000000
VERSION     DATMCV010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686228"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VNU03_10250"
     CDS             complement(<1..224)
                     /locus_tag="VNU03_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HWP74889.1"
                     /translation="MAAVIPDRTVRHRLGVEDLVRYTLVVLFAAILYLFVLYPLLQVI
                     WRSLLANDGTFVGWANYRRYFGTPAIAQSIS"
     gene            complement(228..1286)
                     /locus_tag="VNU03_10255"
     CDS             complement(228..1286)
                     /locus_tag="VNU03_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HWP74890.1"
                     /translation="MHGIAKKFGQFVALRDVSLSILPGEFVCFLGPSGCGKTTLLRIV
                     AGLERQNAGQVMMAGRDVSGLPPSLRNYGIVFQSYALFPNLTVARNVAYGLETRRMPR
                     GDIERRVDELLALVGLAAHKAKYPAQLSGGEQQRIALARALAPSPALLLLDEPLSALD
                     ARVRQSLRHEIRALQRRLGVTTIMVTHDQEEALAMADRIVVMNHGVVEQVGTPVEVYT
                     RPHSPFVARFVGQMNFLDAVAGERAGWARLGAVEMRHGTAGPLAAGTRLTLAIRPEEI
                     LVGPAADGAENRLVTRIRTVQFLGPFTRLGLALPDGARALECDVAATAFAALGAGEGS
                     ELALSLRPDALRVFPVAG"
     gene            complement(1322..>2109)
                     /locus_tag="VNU03_10260"
     CDS             complement(1322..>2109)
                     /locus_tag="VNU03_10260"
                     /inference="COORDINATES: protein motif:HMM:NF020939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HWP74891.1"
                     /translation="IRWVEGGSCRHDAILRYFGDEAETLAGCGRCDVCRGLEAEGEAG
                     DPERVTLIVRKALSGVARVHGRFGLQTVVKLIQGEADERIERAGLTRVPTFGNLKEHP
                     GPWLLALLRRCVSAGWVSFTGLERPVVVLTDEGRAVMKGERPARLLLPPLTRRAAAGP
                     GAGRGGETGRRRPAREAAELDAGAQALFEALRRHRLAVARAEGMAPFIVASDRTLRDI
                     AMLRPRTLAELQEAHGVGPNKAERYGAGFLRIIAEETAKAGPG"
BASE COUNT          293 a          772 c          792 g          252 t
ORIGIN      
        1 gagatcgact gggcgatcgc gggggtcccg aagtagcgcc ggtagttggc ccagcccacg
       61 aaggtgccgt cgttggcgag caggctgcgc cagatcacct gcagcagcgg gtagagcacg
      121 aagaggtaca ggatggccgc gaacagcacg accagcgtgt agcggaccag gtcctcgacg
      181 ccgagccggt ggcggaccgt ccggtcgggg atcaccgcgg ccatgggcta gcccgcgacc
      241 gggaagaccc gcagtgcgtc gggccggagc gagagcgcca gctccgagcc ctcccccgcg
      301 cccagggccg cgaaggcggt cgcggccacg tcgcactcga gcgcgcgcgc accgtcgggg
      361 agcgccaggc cgagccgggt gaagggcccc aggaactgga cggtgcggat gcgcgtgacc
      421 agccggttct cggcgccgtc ggcggcgggg cccaccagga tctcctcggg ccggatggcg
      481 agggtcagcc gggtgccggc cgcgaggggc ccggcggtcc cgtggcgcat ctccaccgcg
      541 ccgagccgcg cccagcccgc gcgctcgccc gcgaccgcgt cgaggaagtt catctggccc
      601 acgaagcgcg ccacgaaggg cgagtgcggc cgcgtgtaca cctcgaccgg cgtccccacc
      661 tgctccacca ccccgtggtt catgaccacg atgcggtcgg ccatggcgag ggcttcctcc
      721 tggtcgtggg tgaccatgat ggtggtgacg ccgagccgcc gctgcagcgc gcggatctcg
      781 tggcggaggc tctggcgcac gcgggcgtcg agcgcggaga gcggctcgtc cagcaagagc
      841 agggcgggcg acggcgcgag ggcgcgggcc agcgcgatgc gctgctgctc gccgcccgag
      901 agctgcgcgg ggtacttggc cttgtgcgcc gcgaggccga ccagggccag cagttcgtcg
      961 accctgcgct cgatgtcccc ccgcggcatc cggcgggtct cgagcccgta ggccacgttg
     1021 cgggccaccg tgagattcgg gaagagcgcg taggactgaa agacgatgcc gtagttgcgg
     1081 aggctgggcg gcagcccgga gacgtcgcgc cccgccatca tgacctggcc cgcgttctgc
     1141 cgctcgagcc ccgcgacgat ccgcagcagc gtggtcttgc cgcagccgct cgggcccagg
     1201 aagcagacga actcgccggg caggatcgag agactcacgt cgcgcagcgc cacgaactgg
     1261 ccgaacttct tggcgatgcc atgcagggcg accgccgccg gcttcgacgc gggcgaggtg
     1321 ctcacccggg gcccgccttc gcggtctctt ccgcgatgat ccggaggaac ccggcgccgt
     1381 agcgctcggc cttgttcggg ccgactccgt gggcctcctg cagctcggcc agcgtgcgcg
     1441 gccgcagcat cgcgatgtcg cgcagggtgc ggtcgctcgc cacgatgaac ggcgccatgc
     1501 cctccgcgcg cgccaccgcc agccggtggc ggcgcagggc ttcgaagagc gcctgggcgc
     1561 cggcgtccag ctcggccgcc tcgcgcgcgg gccggcgacg gccggtctcg ccgccgcggc
     1621 cggcgcccgg tcccgcggcg gcgcgccggg tcaggggcgg caggagaagg cgggccgggc
     1681 gctccccctt catgaccgcg cgcccctcgt cggtcaggac gacgaccgga cgctcgaggc
     1741 cggtgaagct cacccagccc gcggagacgc agcggcgcag cagcgccagc agccagggac
     1801 ccgggtgctc cttcaggtta ccgaaggtgg gcacgcgggt gaggccggcc cgctcgatgc
     1861 gctcgtcggc ctcgccctgg atcagcttga ccacggtctg gagaccgaag cggccgtgca
     1921 cgcgggcgac tcccgagagc gccttgcgca cgatgagcgt cacccgctcc ggatcgccgg
     1981 cctccccttc cgcctcgagc ccgcggcaga cgtcgcaacg gccgcagccg gccagggtct
     2041 cggcctcgtc gccgaagtag cgaagaatcg cgtcgtgacg gcagcttccc ccctcgaccc
     2101 agcgaatca
//
LOCUS       DATMCV010000445         2939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_291722, whole genome shotgun sequence.
ACCESSION   DATMCV010000445 DATMCV010000000
VERSION     DATMCV010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291722"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1261)
                     /locus_tag="VNU03_10265"
     CDS             complement(329..1261)
                     /locus_tag="VNU03_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP74892.1"
                     /translation="MADVQDVLIVGGGAAGYTAGIFAARDRCRALLLEKFSAGGQVLN
                     CEHITNFPGFPQGVAGYTLGPLLQEQATTAGLEVAMNEVTGVRRDGDLLLVETDGGVH
                     ATRTLIVASGSRFTTLGVPGEEEFVGRGISHCAACDGSFFMQKPVIVVGGGDAAVDEA
                     LHLTQYASSVTVLHRRDTLRACASLQERGRSERKISFRWNTALRAIEGADGVERVQVE
                     DVVTGKTAAMPASGVFIYAGLTPNTEFLGGLVPLDARGQIVTDLRMRTRVPGILAAGD
                     VRAESARQLVTSAGDGATAALAAIEYLREGRWPA"
     gene            complement(1275..1778)
                     /locus_tag="VNU03_10270"
     CDS             complement(1275..1778)
                     /locus_tag="VNU03_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWP74893.1"
                     /translation="MPIKVGDRLPDGTLTEFIEQETPGCTVGPNAFQVADLTKGKKIV
                     VFGLPGAFTPTCSAKHVPSYVANFDKLKGKGVDEVWCVSVNDAFVMGAWAKDQKSTGK
                     VRMMADGSAALTKALGLEMDLTARGMGVRSQRYSMLVDNGVVKSLNVEAPGKFEVSDA
                     DTMLKQV"
     gene            complement(1891..2145)
                     /locus_tag="VNU03_10275"
     CDS             complement(1891..2145)
                     /locus_tag="VNU03_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74894.1"
                     /translation="MLSSVQLARLVQGFLAEPGASIDQKAAAVGVRRNELMRAARGTI
                     SRTARQRLLAYFARVADPKVRARGATAPAVPHQLRNRGFT"
     gene            2200..2532
                     /locus_tag="VNU03_10280"
     CDS             2200..2532
                     /locus_tag="VNU03_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWP74895.1"
                     /translation="MKPKLRVWVMFGEDLKFGDGRARLLELIDARGSLKKAAQELEMS
                     YRNAWGYLRDLETAAGFKFVERVPGGGPESGMRLTRAGKRFLERYQKFRSGLDEAARR
                     QFDRAFGA"
     gene            2529..2924
                     /locus_tag="VNU03_10285"
     CDS             2529..2924
                     /locus_tag="VNU03_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HWP74896.1"
                     /translation="MSVEPKYLYLTTVGRRSGRPRRIEIWFTRHESAYYLVAEHGRRS
                     QWVQNLAADPAVRARVGARSFRAHARVVEHAAERELVRTVRRLSEAKYGWGDGLVVEL
                     RPVRRAAPLTPSSRPRSARRRRAGLRPPR"
BASE COUNT          423 a         1011 c         1071 g          434 t
ORIGIN      
        1 ggtgtcggtt gtagcgggcc gacgccgaag cggagcttgc tgccctgcga gaaggccggc
       61 gtggcgagcg ggctcgcgag gagggtcgcg aggaggcaga gcaggagcag cgaggggatg
      121 acacgggtcc acttcatcgt cgagtgtctc ctgccgcggc cacgagtacg accggcggcg
      181 aacggggcga accgcaagtc accgaagcgg atgcggagcg tagcatggag cctcaccgcg
      241 tacgaatcgg acgtggcgat accgagatgg caatcggatt gttatttccg atgcttacag
      301 cacgacggcc ggagccgccg tcgagccgtc aggccggcca gcggccctcg cgcaggtatt
      361 cgatcgcggc cagcgcggcg gtagccccgt caccggccga ggtcacgagt tgccgcgccg
      421 actccgcgcg cacgtcgccc gcggcgagga tgccggggac gcgggtgcgc atgcgcaggt
      481 cggtcacgat ctgcccgcgc gcgtcgagcg gcacgaggcc gcccaggaac tcggtgttgg
      541 gcgtgagccc cgcgtagatg aacaccccgc tggccggcat cgcggcggtc ttgccggtga
      601 cgacgtcctc gacctgcacc cgctcgacgc cgtcggcgcc ctcgatcgcg cgcagcgcgg
      661 tgttccaccg gaacgagatc ttgcgctcgc tgcgtccccg ctcctggagg ctcgcgcagg
      721 cccgcagggt gtcgcggcgg tgcagcaccg tgaccgagga ggcgtactgg gtgaggtgca
      781 gcgcctcgtc gaccgccgcg tcgccgccgc ccaccacgat caccggcttc tgcatgaaga
      841 acgagccgtc gcacgccgcg cagtgggaga tgccgcgccc cacgaattct tcctcgcccg
      901 ggaccccgag ggtggtgaag cgcgagccgc tcgcgacgat cagggtgcgc gtcgcgtgga
      961 cgccgccgtc ggtctcgacc agcaggagat caccatcgcg gcggaccccg gtgacctcgt
     1021 tcatcgcgac ctcgagcccg gccgtggtcg cctgctcctg gagcagcggg cccagggtgt
     1081 agccggcgac cccctggggg aagccgggga agttcgtgat gtgctcgcag ttgagcacct
     1141 ggccccccgc ggagaacttc tcgagcagga gggcccggca gcggtcgcgg gcggcgaaga
     1201 tgccggcggt gtagccggcg gctccgccgc ccacgatcag gacgtcctgg acgtccgcca
     1261 cgtcgggggt ccggctagac ctgcttcagc atcgtgtcgg cgtcgctcac ctcgaacttg
     1321 ccgggcgcct cgacgttcag cgacttcacg accccgttgt ccaccagcat cgagtagcgc
     1381 tgggagcgca cccccatgcc gcgcgcggtc aggtccatct cgaggccgag cgccttggtg
     1441 agtgcggcgc taccgtcggc catcatgcgc accttgccgg tgctcttctg gtccttggcc
     1501 cacgcgccca tgacgaacgc gtcgttgacc gagacgcacc acacctcgtc gacgcccttg
     1561 cccttgagct tgtcgaagtt cgccacgtag ctcggcacgt gcttggcgga gcaggtgggg
     1621 gtgaaggcgc cgggcagacc gaacaccacg atcttcttgc ccttggtcag gtccgccacc
     1681 tggaacgcgt tggggccgac ggtgcagccc ggggtctcct gctcgatgaa ctccgtaagc
     1741 gtgccgtccg gtagacggtc accgactttg atcggcatgg gtctcctcct cgaagaaatg
     1801 gttgtggaac gtgcctcggt tcggccgcga tcagcgagcg gcggctaggg taccacagcg
     1861 ggccgggcgc cgcccggcca ggagcggggc tcatgtaaac cctcggttcc gtagttgatg
     1921 cgggaccgcc ggagcggtgg ccccgcgggc cctcaccttg gggtcggcga cgcgggcgaa
     1981 gtaggccagg agccgctggc gggcggtacg tgagatggtc ccgcgggcgg cgcgcatgag
     2041 ctcgttgcgc cgcaccccga ccgccgccgc cttctggtcg atcgaggcgc ccggctcggc
     2101 caggaatccc tggacgaggc gcgcgagctg cacgctggaa agcattcgtc gatggtacgg
     2161 ctgttcagcc gtgctccgct tgagtaagat tcggttcaga tgaagcccaa gctccgggtg
     2221 tgggtcatgt tcggcgagga cctcaagttc ggggacgggc gcgcccgcct cctcgagctg
     2281 atcgacgcgc gcggctccct caagaaggcg gcccaggagc tcgagatgtc ctatcgcaac
     2341 gcgtggggct acctgcgcga cctggagacg gcggccggct tcaagttcgt ggagcgggtg
     2401 ccgggcggcg ggcccgagag cggcatgcgc ctcaccaggg ccgggaagcg cttcctcgag
     2461 cgctaccaga agttccggag cggcctcgac gaggccgccc gccggcagtt cgaccgcgcc
     2521 ttcggcgcgt gagcgtcgag ccgaagtacc tctacctgac gaccgtcggc cggcgcagtg
     2581 ggcgcccgcg ccggatcgag atctggttca cccgccacga gagcgcctac tacttggtcg
     2641 ccgagcacgg gcgaaggtcc cagtgggtgc agaacctcgc cgcggacccc gcggtccggg
     2701 cccgcgtcgg cgcccggagc ttccgcgctc acgcccgcgt ggtggagcac gccgcggagc
     2761 gtgagctggt gaggacggtg cgccgcctct cggaggccaa gtacggctgg ggcgatgggc
     2821 tcgtggtcga gctccgcccg gtacgtcggg cggcgccgct caccccttcg tcacgacctc
     2881 gatcagcgcg ccgtcggcgc gcggggctcc gaccaccccg atgaccacgg cggccgggt
//
LOCUS       DATMCV010000446         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_556006, whole genome shotgun sequence.
ACCESSION   DATMCV010000446 DATMCV010000000
VERSION     DATMCV010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556006"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VNU03_10290"
     CDS             <1..412
                     /locus_tag="VNU03_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74897.1"
                     /translation="MAMYPGNGQTHMFTEFYTVNAKTKALEDAWKFAKFPGGNGNGDW
                     YVQRQWCLIAGLDNPYPEMYDHPEVTASYDKWIDLKLLREQYKKGRTINAFKESWFSE
                     YDTKAIAIVHNIIRGSSTVPKGLKELVALQKSLV"
     gene            436..1380
                     /locus_tag="VNU03_10295"
     CDS             436..1380
                     /locus_tag="VNU03_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWP74898.1"
                     /translation="MRTRGAGGLRLSEAGFAWLLVLPVVIGIGLVVVVPLAYSFWLSF
                     TDVNLLRTAGPALEAFGIRLPLYRWVGLKNYLQVFDDPLYWSSLWRTLYFVGAFVVEA
                     TLAGMGMALVLNERFAGRPLMRSLLLIPWSLSRIVVGLLWVGLLDFEFGALNALLHQA
                     GLIESYVAFFKNGWSSLNVLVSVYMWNQAPFATLLFLAGMQSIGEDQYAAAEVDGAGY
                     WQRFAHVTLPALRPILFLVLVLCTVNGFLMLDLIYILTMGGPGYDTTTISWLGFQTTF
                     KFFKFGPGTAILYTLTGLCLLMTLLYHRLILSGFEAEA"
     gene            1391..2275
                     /locus_tag="VNU03_10300"
     CDS             1391..2275
                     /locus_tag="VNU03_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWP74899.1"
                     /translation="MRKRRGGAYWARSGRSRLVLYGLVALVSLWLIGPFVWLFVTSVS
                     YQKHLMARPLSFVPPEITLDNYRMITGLMRFHAEGQASKILPSMWNTLVVAGVVTAVN
                     LVLGTTAGYAYARFRFPLKTVSLFALLFTRMLPTIVLIPAFFVIFRALGLQNTLSGLV
                     VAYCSFTLPFTVWIMKAYFETVPADLDKSALVDGCNRLQAYYKVVLPVSGPGLVAGGA
                     FTFMLCWNEFVVAQVLNTKPGTTTLPPVIAGMNGQINIDYSVIAASGFLGALPAVLLV
                     LVFQKYMVQGLTAGSVKG"
     gene            2329..2907
                     /locus_tag="VNU03_10305"
     CDS             2329..2907
                     /locus_tag="VNU03_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWP74900.1"
                     /translation="MDQAPLSVRRWSRAEYERLVDLGVFHAEPVELIGGQLIVAEPQN
                     SPHATAVGAADDALRAVLPAGFIVRTQMPVALDEESAPEPDLAVVAGRRADYQVRHPA
                     RPVLVVEVADSSLRFDREDKGSLYARARVLEYWIVNLIDRVVEVYRDPGPDASAAHGW
                     RYRSVVHLPPTGTVEPVALPSARVRVADLLLR"
     gene            complement(2919..>3101)
                     /locus_tag="VNU03_10310"
     CDS             complement(2919..>3101)
                     /locus_tag="VNU03_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74901.1"
                     /translation="GRGHVGPAAGGIGSHGVVMWRITGGDGRFAGAQGLITSNFTVSP
                     DGLVTDDHFTRLYLPE"
BASE COUNT          430 a         1092 c         1021 g          558 t
ORIGIN      
        1 gatggcgatg taccccggca acgggcagac ccacatgttc accgagttct acaccgtgaa
       61 cgccaagacc aaggccctcg aggacgcgtg gaagttcgcc aagttccccg gcggcaacgg
      121 caacggggac tggtacgtgc agcggcagtg gtgcctgatc gccggcctcg acaacccgta
      181 ccccgagatg tacgaccacc ccgaggtcac cgcgtcctac gacaagtgga tcgacctcaa
      241 gctcctgcgc gagcagtaca agaagggccg caccatcaac gcgttcaagg agtcgtggtt
      301 ctcggagtac gacaccaagg ccatcgcgat cgtccacaac atcatccgcg gctcatcgac
      361 ggtgccgaaa ggcctcaagg agctggtggc gctgcagaag tccctggtgt aacccgcggc
      421 tcgcccgcgg tccccatgcg aacgcgcggc gcgggcgggc tccgtctctc cgaggcgggg
      481 ttcgcctggc tgctcgtcct gccggtcgtg atcgggatcg gcctcgtggt ggtcgtcccg
      541 ctcgcctact ctttctggct ctcgttcacc gacgtcaatt tgctgcgcac cgcgggcccc
      601 gcgctggagg ccttcgggat ccgtctgccg ctctaccgct gggtgggcct caagaactac
      661 ctccaggtct tcgacgaccc gctctactgg agctcgctct ggcgcaccct ctacttcgtg
      721 ggcgcgttcg tggtcgaggc gacgctggcc ggcatgggga tggcgctggt gctcaacgag
      781 cgattcgcgg ggcggccgct gatgcggagc ctcctgctga tcccgtggtc gctctcccgg
      841 atcgtggtcg gcctcctgtg ggtcggcctg ctcgacttcg agttcggggc gctgaacgcg
      901 ctgctccacc aggcgggctt gatcgagtcc tacgtcgcgt tcttcaagaa cgggtggagc
      961 tcgctcaacg tcctggtctc ggtctacatg tggaaccagg cgcccttcgc cacgctcctg
     1021 ttcctggccg gcatgcagtc gatcggagag gaccagtacg ccgccgccga agtcgacggg
     1081 gccggctact ggcagcgctt cgcccacgtc acgctgcccg ccctgcggcc gatcctgttc
     1141 ctggtgctgg tgctctgcac cgtgaacggc ttcctgatgc tggacctgat ctacatcctc
     1201 accatgggcg gccccggcta cgacaccacc accatctcgt ggctcggctt ccagaccacc
     1261 ttcaagttct tcaagttcgg ccccggcacc gcgatcctct acaccctgac cgggctctgc
     1321 ctgctcatga ccctgctcta ccaccggctg atcctgtccg gcttcgaggc ggaggcctag
     1381 gatgatgagc gtgcggaagc gccggggcgg cgcctactgg gcgcgctcgg ggcggagccg
     1441 cctcgtcctc tacgggctcg tcgccctcgt gagcctctgg ctgatcggcc ccttcgtctg
     1501 gctcttcgtg acgagcgtct cctaccagaa gcacctgatg gcgcggccgc tgtcgttcgt
     1561 cccgccggag atcaccctcg acaactaccg catgatcacg gggctgatgc gcttccacgc
     1621 cgaggggcag gcgtccaaga tcctgccgtc gatgtggaac accctggtgg tggcgggggt
     1681 ggtgaccgcg gtgaacctgg tgctgggcac caccgccggt tacgcctacg cgcgcttccg
     1741 gtttcccctc aagacggtgt ccctcttcgc gctgctcttc acccgcatgc tgccgaccat
     1801 cgtgctcatc cccgccttct tcgtcatctt ccgcgccctc ggcctgcaga acacgctctc
     1861 gggcctggtc gtcgcctact gctcgttcac cctgccgttc acggtgtgga tcatgaaggc
     1921 ctacttcgag acggtgcccg ccgacctcga caagagcgcg ctcgtcgacg gctgcaaccg
     1981 gctgcaggcc tactacaagg tcgtgctgcc ggtgtcgggg ccgggcctgg tggcgggcgg
     2041 ggcgttcacg ttcatgctct gctggaacga gttcgtggtg gcccaggtgc tcaacaccaa
     2101 gccgggcacc accacgctgc ccccggtcat cgcgggcatg aacggccaga tcaacatcga
     2161 ctactcggtg atcgcggcgt ccggcttcct gggggcgctc cccgcggtcc tgctcgtgct
     2221 cgtcttccag aagtacatgg tgcagggcct caccgccggg tcggtgaagg ggtagcgtct
     2281 ccgcgggtag acgttgaccg ccgggcaccc cttcggtagc gtggcgtcat ggatcaggct
     2341 ccgctgagcg tgcgccgctg gagccgcgcg gaatacgagc gcctcgtcga tctcggggtg
     2401 tttcacgccg agccggtgga gctgatcggc gggcagctca tcgtcgccga gccacagaac
     2461 agcccgcacg ccaccgcggt cggagcggcc gatgatgccc tgcgggcggt gctgcccgcg
     2521 ggcttcatcg tgaggaccca gatgccggtg gccctcgacg aggagtccgc gcccgagccg
     2581 gatctggcgg tcgtggccgg gcggcgcgcg gactaccagg tgcgccatcc ggcccgcccg
     2641 gtcctggtcg tggaagtagc cgactcgagc ctgcgcttcg accgcgagga caagggcagc
     2701 ctctacgccc gcgcccgcgt tctggagtac tggatcgtca acctgatcga ccgcgtcgtg
     2761 gaggtgtatc gcgacccggg accggacgca tcggccgcgc acggctggcg gtatcgctcg
     2821 gttgtgcatc tccctcccac cggaacggtg gagccggtgg cgctgccgtc ggcccgggta
     2881 cgggtcgccg acctcctgct tcggtgagcc gccgcctgct actccggcag gtagaggcgg
     2941 gtgaagtggt cgtcggtgac gaggccgtcc gggctcaccg tgaagttgga cgtgatcagc
     3001 ccctgcgcgc ccgcgaagcg gccgtcgccg ccggtgatgc gccacatcac cacgccgtgg
     3061 ctcccgatgc cgcccgcggc ggggccgacg tgaccccggc c
//
LOCUS       DATMCV010000447         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_686346, whole genome shotgun sequence.
ACCESSION   DATMCV010000447 DATMCV010000000
VERSION     DATMCV010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686346"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..462
                     /locus_tag="VNU03_10315"
     CDS             67..462
                     /locus_tag="VNU03_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HWP74902.1"
                     /translation="MSLEPKYLYLTTIGRRSGRPRRIEIWFTRHASAYYLVAEHGRRS
                     QWVQNLAADPAVRARVGARSFRAHARVVDPVAERELVRAVRRLSEAKYGWGDGLVIEL
                     RPIRRAAPLTPSSRPRSARRRRAGRRPPR"
     gene            complement(398..>2431)
                     /gene="selD"
                     /locus_tag="VNU03_10320"
     CDS             complement(398..>2431)
                     /gene="selD"
                     /locus_tag="VNU03_10320"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HWP74903.1"
                     /translation="EIDVRPLALRAGARCIVAAATGVDPTARRLVLDGRPPIAYDTVS
                     FDVGSTVAGLEIPGAREHAIPTRPIGEFVRRVDEVLAAARGRASFRVLVVGAGAGGVE
                     VAFALTARLRGEPGRRVDVLLLEAGPRVLPGYAASAARRVQAAADARGITIRCGAAVA
                     RAEAGAVHLVGGERLAADATVWVTGAAALPIFSGSGLETDDRGFVRIRPTLQCRGHDD
                     VFAVGDCAAWTAGPPLAKAGVYAVRQGPVLARNLMARARGGRLRVYRPQRDFLSLLNL
                     GDGRAIGTKWGASAEGASLFSLKDWIDRRFVRRFQVLGPDDAVTPDFAASPMPGGDML
                     CGGCAAKVGESALTRALERLGPASHPSVILGLTRPDDAAAVETERGEIVAATVDSFRA
                     FADDPYLVGRVAAVNAISDLWAKGVAPRFALAQVTVPDAEPARAEETLYQVMAGARAG
                     LDADAVTLVGGHTTIGPELVVGFAVWGLAGSADALIRLGGLAPGDQLVLTKPLGTGVL
                     LQADMRGVARGAWIEACCASMLRSNGPAARAAGPLRPSAATDVTGFGLAGHLGSMLRA
                     SKASAVLDLAALPALPGALSLLGRGVRSTAHPENAKARRAMWVDDAAARRPALDLLFD
                     PQTSGGLLLGIPAERARALLQALRAAGDPTAAVIGAVGAPRADGALIEVVTKG"
BASE COUNT          315 a          926 c          938 g          252 t
ORIGIN      
        1 ctaccagaag ttccggagcg gcctcgacga ggccgcccgg cggcagttcg accgcgcctt
       61 cggcgcgtga gtctcgagcc gaagtacctc tacctgacga ccatcggccg gcgcagcggg
      121 cgcccgcgcc ggatcgagat ctggttcacc cgccacgcga gcgcctacta cctggtcgcc
      181 gagcacgggc gaaggtccca gtgggtgcag aatctcgccg cggaccccgc ggtccgggcc
      241 cgcgtcggcg cccggagctt ccgcgctcac gcccgcgtgg tggaccccgt cgcggagcgc
      301 gagctggtga gggcggtgcg tcgcctctcg gaggccaagt acggctgggg tgacgggctc
      361 gtgatcgagc tccgcccaat acgtcgggcg gcgccgctca ccccttcgtc acgacctcga
      421 tcagcgcgcc gtcggcgcgc ggggcgccga ccgccccgat gaccgcggcg gtcgggtcgc
      481 cggccgcgcg cagcgcctgc agcagcgccc gagctcgctc ggcggggatg ccgagcagca
      541 ggccgcccga cgtctgcggg tcgaagagca ggtcgagcgc gggccgccgc gccgccgcat
      601 cgtccaccca catcgcgcgg cgggccttgg cgttctcggg atgcgcggtg ctccgcacgc
      661 cgcggccgag cagcgagagc gcgcccggca gcgccgggag cgcggcgagg tcgagcaccg
      721 ccgaggcctt gctcgcgcgc agcatcgacc cgaggtggcc ggccaggccg aagccggtga
      781 cgtcggtggc cgccgaggga cgcagggggc cggccgcccg cgcggcgggg ccgttcgagc
      841 ggagcatgga ggcgcagcac gcctcgatcc acgcgccgcg cgccacgccc cgcatgtcgg
      901 cctgcaggag cacgccggtg ccgagcggct tggtcagcac gagctgatcg cccggggcca
      961 ggcctccgag tcgaatcagg gcgtccgcgg agccggccag gccccacacc gcgaagccca
     1021 ccactagctc gggcccgatc gtggtgtgcc cgccgaccag ggtgaccgcg tccgcatcga
     1081 ggccggcgcg ggcgccggcc atcacctggt agagcgtctc ctcggcgcgc gcgggctcgg
     1141 cgtccggcac cgtgacctgg gccagcgcga accgcggcgc cacgcccttg gcccacaggt
     1201 cgctgatcgc gttgaccgcg gccacgcgcc cgaccaggta ggggtcgtcg gcgaaggcgc
     1261 ggaaggagtc gacggtggcg gccacgatct cgccccgctc ggtctcgacc gcggccgcgt
     1321 cgtcgggccg ggtgaggccg aggatcaccg acgggtggct cgcgggcccg aggcgctcga
     1381 gcgcgcgcgt cagggcggac tcgccgacct tggccgcgca gccgccgcag agcatgtcgc
     1441 cgcccggcat cggggacgcg gcgaagtcgg gggtgaccgc gtcgtcgggg cccaggacct
     1501 ggaatcggcg gacgaagcgg cggtcgatcc agtccttgag agaaaagagc gaggcgcctt
     1561 cggccgaggc gccccacttg gtgccgatcg cgcgcccgtc gcccaggttg agcagcgaga
     1621 ggaagtcgcg ctggggtcga tagacgcgga gccggccgcc gcgggcgcgc gccatcaggt
     1681 tgcgcgcgag gaccgggccc tggcggaccg cgtagacccc ggccttggcc agcggcgggc
     1741 ccgcggtcca ggcggcgcag tcgccgaccg cgaagacgtc gtcgtgaccc cggcactgga
     1801 gcgtgggcct tatcctgacg aagccccgat cgtccgtctc caggcccgac cccgagaaga
     1861 tcggcagggc ggcggcgccg gtcacccaca cggtggcgtc ggccgcgagg cgctcgccgc
     1921 ccaccagatg caccgcgccg gcctccgcgc gtgccaccgc ggcgccgcag cggatcgtga
     1981 tgccgcgcgc gtccgccgcg gcctggaccc gacgcgccgc gctcgccgcg tagcccggga
     2041 ggacgcgcgg ccccgcctcg agcaggagga cgtcgacgcg gcggcccggc tcacctcgga
     2101 gccgcgcggt gagggcgaag gcgacctcca cgccgcccgc gccggcgccg accaccagca
     2161 cgcgaaagga cgcgcggcct cgcgcggcgg ccagcacctc gtccacccgc cggacgaact
     2221 cgccgatggg ccgcgtgggg atcgcgtgct cacgcgcgcc cgggatctcg agcccggcga
     2281 cggtcgagcc cacgtcgaac gacacggtgt cgtaggcgat gggcggccgg ccgtcgagca
     2341 cgagccggcg cgcggtcggg tcgacaccgg tggccgccgc cacgatgcag cgagcgcccg
     2401 ctcgcagggc cagcgggcgc acgtcgatct c
//
LOCUS       DATMCV010000448         4477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_92185, whole genome shotgun sequence.
ACCESSION   DATMCV010000448 DATMCV010000000
VERSION     DATMCV010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92185"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VNU03_10325"
     CDS             <1..970
                     /locus_tag="VNU03_10325"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP74904.1"
                     /translation="GRYRGIGLANYIEIATGAPRERAQVTARPDGFIDVIIGTLSAGQ
                     GHETAFPQLIAEWLGVEPDRVRLIAGDTDRVAVGGGSHSGRSMRLGGVVMAKASDRLV
                     ARGARLAAWLLESAAEDVEFAHRRFAVRGTDRAVDLFAVAAAALRDDAPADLRGPLEG
                     ECDETVSTPSFPYGSAVCEVEVDPETGAVEIVRYSSVDDVGRAINPMIVHGQTHGGIA
                     QGVGQALWELSDYDPESGQLRSATFMEYAMPRADLLPSFATEISEVESTSNPLGLRGG
                     GEGGTTPALGAVVNAVVDALAHLGVEHLEMPVTPERVWRAIRAAAR"
     gene            complement(989..1711)
                     /locus_tag="VNU03_10330"
     CDS             complement(989..1711)
                     /locus_tag="VNU03_10330"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HWP74905.1"
                     /translation="MDGAAAGRPPGVEFHTFTVVGRCRRTGRLGVGTATGSLAVGARV
                     PHVRAGLGAVAIMAIADARLGQMALRLLDLGYKAPAVVEELVRADPYAEYRQLGVIDG
                     DGFAAARTGGKNREWAGQRVRDDYIVLGNYLTGEHILEAMERAFTANPDEDLEERLLR
                     GIEAGRDAGGQHGGQQSAALLVYEDRSFAHVDLRVDVHPEPVGELRRVFDAYRPAIPY
                     YNLRQVDARVPPLPDWLRDRPR"
     gene            complement(1711..2910)
                     /locus_tag="VNU03_10335"
     CDS             complement(1711..2910)
                     /locus_tag="VNU03_10335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP74906.1"
                     /translation="MTQTADVVVIGGGVNGVSIAHALAGRGVRVVLVEKGALASGASG
                     RSSALVRMHYTNEWDARLAWASFPVFRNWPELMGGPPVFTHTGFVNVVGPDHVENLRR
                     NVAMLQGIGVPTRAIGPDELRALQPYAHVDDVGAAAYEPESGYANPAETVEGFRRRAT
                     ELGARIVQWTAVTRIDRSESRVRGVETSAGRIEAGSVVVAAGAWSRRLCDEMGLALPA
                     RPKAIDTVAVSRPTELRDPHMVYIDNVLGNYFRPEAGGLSLVGIPCQEWDIDPDTLGT
                     GLPPGAAELGASLITHRIPVMERATLARGYRAFDGYSADRHAILGGVDGIDGLYLATA
                     FSGSGFKIAPAVGACMAELITEGRAKTVDIAPFGLGRFAAGRTVEGPYPYAVRPDHVD
                     PAAAGRA"
     gene            complement(2921..3739)
                     /locus_tag="VNU03_10340"
     CDS             complement(2921..3739)
                     /locus_tag="VNU03_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP74907.1"
                     /translation="MRLGVSVPTSRPDGSPLTGDSLVGSARGIEQAGFDSAWFFDAIG
                     RGFMLPDPLIGAAVAAAVTTRIEIGTGVLQVPLRNPVELAHRILTAHLVCRGRLLLGV
                     GAGSTPGDFEALGLDFTQRMRTLEESLATMRKLWRGEKVGAADLTPWPAALGGPAVLI
                     GSWKGERWIPRAAREFDGWIASGAKTSLQALTEGIGRYRAAGGKRAIVTNIAVDLTAP
                     TEPLPASAPFHLRCAPDAAARRLQQVRDLGFDDAILVLKTPTEAGLAAARPLLR"
     gene            complement(3744..4151)
                     /locus_tag="VNU03_10345"
     CDS             complement(3744..4151)
                     /locus_tag="VNU03_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP74908.1"
                     /translation="MSDPKVVRLDETERVSFGPLSHYQPIIGDDQGTTPVRTGIQTAQ
                     PGYVAPVHAHPYLEILHILDGTAEAWIDGQEDRAVLLRKGDTIALPPDTPHAFRVVGD
                     QELRLLGTHTSPTRIVRYKDGGSSDARGYRVQD"
     gene            complement(4206..>4477)
                     /locus_tag="VNU03_10350"
     CDS             complement(4206..>4477)
                     /locus_tag="VNU03_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74909.1"
                     /translation="LLFAAFAASAAGGNALVATHGYGYTAPGSLLLHGLTLGLPMVAA
                     SLAVQGAARRIGSRAGLYALGLAAALLGWVAGIVAAVALVHGLLR"
BASE COUNT          578 a         1682 c         1660 g          557 t
ORIGIN      
        1 ggggcgctac cgcggcatcg gcctcgccaa ctacatcgag atcgccaccg gcgcgccgcg
       61 cgagcgggcg caggtcaccg cgcggccgga cggcttcatc gacgtgatca tcggcacgct
      121 ctcggccggg cagggccacg agaccgcctt cccccagctc atcgcggagt ggctcggcgt
      181 cgagccggat cgcgtgcgcc tgatcgcggg cgacaccgac cgggtggcgg tgggcggcgg
      241 gtcacactcc ggccgctcca tgcggctggg cggtgtggtg atggccaagg cctccgaccg
      301 cctcgtggcg cggggcgccc ggctcgcggc gtggctgctc gaatccgcgg cggaggacgt
      361 ggagttcgcg caccggcgct tcgcggtgcg cgggaccgac cgcgcggtcg atctcttcgc
      421 ggtcgcggcc gccgcgctcc gggacgacgc gccggccgac ctgcgcggcc ccctcgaggg
      481 cgagtgcgac gagacggtga gcacgccgtc cttcccctac ggctcggcgg tgtgcgaggt
      541 cgaggtcgat ccggagaccg gcgcggtcga gatcgtgcgc tacagcagcg tggacgacgt
      601 gggacgcgcc atcaacccga tgatcgtcca cggccagacc cacggcggca tcgcgcaggg
      661 agtcggccag gcgctctggg agctctccga ctacgacccg gagtcgggcc agctccgctc
      721 cgccaccttc atggaatacg cgatgccgcg cgccgacctg ctgccgtcgt tcgccaccga
      781 gatcagcgag gtcgagtcga cctcgaaccc gctcgggctg cgcggcggcg gcgagggcgg
      841 gaccacgccc gcgctcggcg ccgtggtcaa cgcggtggtc gacgccctcg cccacctggg
      901 ggtggagcac ctcgagatgc cggtcacgcc cgagcgggtg tggcgcgcca tccgcgcggc
      961 cgcgcgctga ccgggagccg ccgcgagctc agcgcggccg gtcgcgcagc cagtcgggga
     1021 gcggcggcac ccgcgcgtcg acctggcgca gattgtagta gggaatcgcg gggcggtagg
     1081 cgtcgaacac gcgccgcagc tcgcccaccg gctccgggtg cacgtcgacg cggagatcca
     1141 cgtgcgcgaa ggaccggtcc tcgtagacca gcagggcggc ggactgctga cccccgtgct
     1201 ggccacccgc gtcgcggccc gcctcgatgc cgcgcagcag gcgctcctcc aggtcctcgt
     1261 cgggattcgc ggtgaacgcg cgctccatcg cctccaggat gtgctcgccg gtcaggtagt
     1321 tgccgagcac gatgtagtcg tcgcgcaccc gctggccggc ccactcgcgg ttcttgcccc
     1381 cggtgcgggc ggccgcgaag ccgtcgccgt cgatgacccc gagctggcgg tactccgcgt
     1441 agggatcggc gcgcaccagc tcctccacca ccgcgggggc cttgtacccc agatcgagga
     1501 gccggagcgc catctggccc aggcgcgcgt cggcgatggc catgatcgcg accgcgccga
     1561 ggccggcccg cacgtgcggc acccgggctc cgaccgcgag ggagccggtc gcggtgccca
     1621 ccccgagccg cccggtgcgc cggcagcggc cgaccaccgt gaaggtgtgg aactcaacgc
     1681 ccggcgggcg gccggccgcc gcgccgtcca tcacgcgcgg ccggccgcgg ccggatcgac
     1741 gtgatcgggc cgcaccgcgt acggatacgg cccctcgacg gtgcggcccg ccgcgaagcg
     1801 gccgagcccg aacggcgcga tgtcgacggt cttggcccgg ccctccgtga tcagctcggc
     1861 catgcacgcg cccaccgccg gggcgatctt gaagccggag ccgctgaacg cggtggccag
     1921 gtagagcccg tcgatgccgt ccacgccgcc caggatcgcg tggcgatccg cgctgtagcc
     1981 gtcgaaggcg cgatagccgc gcgccagcgt ggcgcgctcc atgaccggga tgcggtgcgt
     2041 gatcagcgag gcgccgagct cggccgcccc gggcggcagg ccggtgccga gcgtgtccgg
     2101 atcgatgtcc cactcctggc acgggatgcc gacgaggctg aggccgcccg cctccggccg
     2161 gaagtagttg cccagcacgt tgtcgatgta gaccatgtgc gggtcgcgca gctcggtggg
     2221 ccggctcact gccaccgtgt cgatggcctt gggccgcgcg ggaagggcca ggcccatctc
     2281 atcgcagagc cgccgcgacc aggcgcccgc ggccaccacc acgctcccgg cctcgatgcg
     2341 cccggcggag gtctcgacgc cgcggacgcg gctctcgcta cggtcgatgc gcgtcaccgc
     2401 ggtccattgc acgatgcgcg cgcccagctc ggtggcgcgg cggcggaagc cctcgacggt
     2461 ctcggccgga ttcgcgtagc cgctctcggg ctcgtaggcc gccgcgccca cgtcgtcgac
     2521 gtgggcgtag ggctggagcg cccgcagctc gtccggaccg atcgcgcgcg tcggcacccc
     2581 gatgccctgg agcatcgcca cgttgcgccg caggttctcc acgtggtccg ggcccaccac
     2641 gttgacgaag ccggtgtggg tgaagacggg cggcccgccc atcagctcgg gccagttgcg
     2701 gaagaccggg aagctggccc aggccaggcg cgcgtcccac tcgttggtgt agtgcatgcg
     2761 cacgagcgcg ctcgagcgcc ccgaggcccc gctcgcgagc gcgcccttct ccaccagcac
     2821 gacccgcacc ccgcgcccgg ccagcgcgtg cgcgatgctc acgccgttga cgccgccgcc
     2881 gatcaccacc acgtccgccg tctgcgtcat ggggccgggc tcagcgcagg agcggccgcg
     2941 cggcggcgag ccccgcctcg gtcggcgtct tgagcaccag gatggcgtcg tcgaagccca
     3001 ggtcgcgcac ctgctgcagg cgccgggccg ccgcgtcggg agcacagcgc aggtggaacg
     3061 gcgcgctggc cgggagcggc tcggtgggcg cggtgagatc caccgcgatg ttggtcacga
     3121 tcgcgcgctt gccgcccgcg gcgcgatagc ggccgatccc ctcggtgagc gcctgcaggc
     3181 tggtcttggc ccccgaggcg atccagccgt cgaactcccg cgccgcccgc gggatccagc
     3241 gctcgccctt ccagctgccg atcagcaccg cgggcccgcc cagcgccgcc ggccacgggg
     3301 tgagatcggc cgcgccgacc ttctcgccgc gccacagctt ccgcatcgtc gcgagcgact
     3361 cctcgagcgt gcgcatgcgc tgggtgaagt cgaggccgag cgcctcgaag tcgcccgggg
     3421 tggagcccgc gcccaccccg agcaggagcc gcccccggca gacgagatgc gcggtgagga
     3481 tgcgatgcgc cagctccacc gggttgcgga gcgggacctg cagcacgccg gtaccgatct
     3541 cgatgcgcgt ggtcaccgcg gccgcgaccg cggcgccgat gaggggatcc ggcagcatga
     3601 acccgcgccc gatcgcgtcg aagaaccagg cgctgtcgaa gccggcctgc tcgatcccgc
     3661 gcgcgctgcc gaccagcgag tccccggtga gcggggagcc gtccgggcgc gacgtcggga
     3721 cgctgacccc gagcctcacg cgatcaatcc tgcacccggt agccgcgcgc gtcgctcgag
     3781 ccgccgtcct tgtagcggac gatccgggtc ggcgaggtgt gggtcccgag caggcgcagc
     3841 tcctggtctc ccaccacccg gaacgcgtgc ggggtgtcgg gcggcagggc gatggtgtcg
     3901 cccttccgga gcagcaccgc gcggtcctcc tggccgtcga tccaggcctc cgcggtcccg
     3961 tcgaggatgt gcaggatctc gaggtaggga tgcgcgtgca ccggcgccac gtagcccggc
     4021 tgcgcggtct ggatgccggt gcgcaccggc gtcgtgccct ggtcgtcgcc gatgatgggc
     4081 tgatagtggg acagcgggcc gaagctcacc cgctcggtct cgtcgagccg gaccaccttg
     4141 gggtcgctca tgtgggtcgc ctcctcggtg cgaattctag cccaggcggg ggagcaccgc
     4201 gcggctcatc gcaggagccc gtggacgaga gccaccgcgg ccacgatgcc cgccacccac
     4261 ccgagcagcg cggccgcgag cccgagcgcg tagagccccg cgcgcgagcc gatgcgccgg
     4321 gccgcaccct gcacggccag gctcgccgcg accatcggca gcccgagagt gagcccgtgg
     4381 agcaagaggc tccccggcgc ggtgtagccg tagccgtggg tcgcgaccag cgcgttgccg
     4441 ccggccgcgc tcgccgcgaa tgcggcgaag agcagaa
//
LOCUS       DATMCV010000449         2228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_890513, whole genome shotgun sequence.
ACCESSION   DATMCV010000449 DATMCV010000000
VERSION     DATMCV010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890513"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="VNU03_10355"
     CDS             <1..1501
                     /locus_tag="VNU03_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HWP74910.1"
                     /translation="DRHLAEGRGDRTAFRFHGRVVSYAEVALRANRLGNALLARDVQM
                     EQRVLLALPDRPEFAEAFWGAMKIGAVPVPVSDALPADELAFMLSDSRARAVIASEDA
                     APRILPVRARCPALQSVIVVGGRRRGGLEYERLLERASPELAPADTSRDDVALWLYTS
                     GSTGRPKAAVHLHHDLVHAAELVGRGTFGIGPDDRIFSASKLHFAFGLGNSLYFPALV
                     GAASILVPERLEAERVFELIQAERPTVLFAVATVYRRLLQVENAERRFDCSSLRLCVS
                     SGETLPAALFHAWQARFGLELLDVVGSTEALHDFIANRPGAAQAGSSGQVIPGFEARL
                     VDDQGRPVAPGMIGQLLIKGDSTSPGYWNRHERTKATMQGDWLRTGDMFYQDTDGYFF
                     FCGRTDDMLKVGGMWVAPAEVEAAVGDHPAVLEVAVVGRGDADGLTKPHAYCVLRDGI
                     SGSDALAAELREGVRKRLGGYKSPRWVDFVAELPKTTTGKIQRFALRQR"
     gene            1503..>2228
                     /locus_tag="VNU03_10360"
     CDS             1503..>2228
                     /locus_tag="VNU03_10360"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74911.1"
                     /translation="MRVVVERVAKTFHDRRGRDVIALHQVDFAVESREFVALLGPSGC
                     GKSTLLSIIAGLLPASAGRVYFEGEPPSGRAPVTATVFQEFALFPWRTVRANVQLGLE
                     ELGITGLERVERTRRYLDMTGLDGFEGKYPHQLSGGMRQRVGIARALAVEPAVLLMDE
                     PFSALDAQTRTLMMDELLGIWERTRQTILYVTHNIQEAVFMADRVIVLSRRPGEVLDV
                     VPIELPRPRREGQQGDPIFVQTVN"
BASE COUNT          287 a          789 c          819 g          333 t
ORIGIN      
        1 cgatcgccac ctggccgagg gccgcggcga tcgtaccgcc ttccgcttcc acgggcgcgt
       61 cgtcagctac gccgaggtcg cgctgcgcgc gaaccggctg ggcaacgcgc tgctcgcccg
      121 cgacgttcag atggagcagc gcgtgctcct ggccctgccg gatcgccccg agttcgccga
      181 ggcgttctgg ggcgcgatga agatcggtgc ggtgccggtg ccggtgagcg acgcgctccc
      241 cgccgacgag ctcgcgttca tgctgagcga cagccgcgcc cgcgcggtga tcgcgtcgga
      301 ggacgcggcg ccgaggatcc tcccagtccg ggcgcggtgc ccggcgctcc agtcggtgat
      361 cgtggtgggt gggcgccggc gcggcggtct cgagtacgag cggctgctcg agcgggcctc
      421 gcccgagctg gccccggccg acacgagccg cgacgacgtg gcgctctggc tctacacctc
      481 gggctcgacc gggcggccca aggcggcggt gcatctccac cacgacctgg tgcacgcagc
      541 ggagctggtg gggcggggga ccttcggcat cgggcccgac gatcggatct tctcggcctc
      601 caagctccac ttcgcgttcg ggctgggcaa ctcgctctac ttcccggccc tggtgggcgc
      661 ggcctcgatc ctggtgccgg agcggctcga ggcggagcgc gtgttcgagc tgatccaggc
      721 cgagcggccc accgtgctgt tcgcagtggc caccgtctac cggcgactgc tccaggtcga
      781 gaacgcggag cggcgattcg attgctcctc gctgcggctc tgcgtctcct cgggcgagac
      841 gctgcccgcc gcgctcttcc acgcctggca ggcgcgcttc ggcctcgagc tgctcgacgt
      901 ggtgggctcg acggaggcgc tccacgactt catcgccaac cgtcccgggg cggcgcaggc
      961 cggctcgagc gggcaggtga tccccggctt cgaggcgcgc ctggtcgacg accagggccg
     1021 tccggtggcg cccggcatga tcggccagct cctgatcaag ggcgactcga cctcgcccgg
     1081 ctactggaac cgccacgagc gcaccaaggc caccatgcag ggcgactggc tccgcaccgg
     1141 cgacatgttc taccaggaca ccgacggcta cttcttcttc tgcgggcgca ccgacgacat
     1201 gctgaaggtg ggcggcatgt gggtggcgcc cgcggaggtg gaggcggccg tgggcgacca
     1261 tccggccgtc ctggaggtgg cagtggtcgg gcggggcgac gccgacggcc tcaccaagcc
     1321 gcacgcctac tgcgtgcttc gcgacgggat ctccgggtcg gacgcgctcg ccgccgagct
     1381 gcgagaaggc gtgcgcaagc gcctgggcgg ctacaagtcg ccgcgctggg tcgacttcgt
     1441 ggccgagctg ccgaagacca cgaccggcaa gatccagcgg ttcgcattgc ggcagcgctg
     1501 agatgcgcgt cgtcgtcgag cgcgtcgcca agaccttcca cgaccgccgg ggccgcgacg
     1561 tgatcgcgct ccaccaggtg gacttcgcgg tcgagtcgcg cgagttcgtg gccctgctgg
     1621 gcccgtcggg ctgcggcaag tccacgctcc tcagcatcat cgcggggctc ctgcccgcca
     1681 gcgcggggcg ggtctacttc gagggtgagc cgccctccgg ccgggcgccc gtgaccgcca
     1741 ccgtgttcca ggagttcgcc ctcttcccct ggcgcacggt acgggccaac gtgcagctcg
     1801 gtctcgagga gctgggcatc accgggctcg agcgggtcga gcggacgcgg cgctacctcg
     1861 acatgaccgg cctggacggg ttcgagggca agtacccgca ccagctctcg ggcggcatgc
     1921 gccagcgggt gggcatcgcg cgggccctcg cggtggagcc ggcggtgttg ctgatggacg
     1981 agcccttctc cgccctcgac gcgcagacgc gcaccctgat gatggacgag ctgctcggca
     2041 tctgggagcg tacgcggcag accatcctct acgtcacgca caacattcag gaggcggtgt
     2101 tcatggccga ccgggtgatc gtgctctcgc ggcggccggg cgaggtgctc gacgtcgtgc
     2161 cgatcgagct gccgcgcccg cgacgcgagg ggcagcaggg cgacccgatc ttcgtgcaga
     2221 ccgtgaac
//
LOCUS       DATMCV010000450         3660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_819569, whole genome shotgun sequence.
ACCESSION   DATMCV010000450 DATMCV010000000
VERSION     DATMCV010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819569"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..991)
                     /locus_tag="VNU03_10365"
     CDS             complement(17..991)
                     /locus_tag="VNU03_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP74912.1"
                     /translation="MIQVDNLTKRYGPVTAIQDVSFSVDKGRIVGFLGPNGAGKSTTM
                     KILSCFMPATGGTARVAGYDVFSQSLEVRRRIGYLPESAPLYPDLSVSSYLDFVAEIK
                     GVGRAERRGRVADVMERCFITDMQNRLIGKLSKGYRQRVGLAQALLGDPEVLILDEPT
                     IGLDPKQISEIRALIRSLAGQHTVILSTHILPEVSMVCDGIVIINHGRIVAQGTESEL
                     VQQVFPTARIEVRVTNASGDVAGALRAVPGVVAVEPLASRDGSVGFVVESERDRDVRG
                     DLVRLVTGKNWALQELHQVGMSLEEVFIRVVAGEQVAPDVVAAEEEAR"
     gene            complement(1040..2716)
                     /locus_tag="VNU03_10370"
     CDS             complement(1040..2716)
                     /locus_tag="VNU03_10370"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWP74913.1"
                     /translation="MPTSTPPRPDADRKPRPIAQIARDLGLGDDEWIPYGHDKAKVRV
                     GALAARAGQPDGKLVVVSSITPTPAGDGKTTMTIGLGQALWRVGGRPVIALREPSIGP
                     TLGMKGGGTGGGRSQVVPMDEINLHFTGDFHAITSAHNLLAAALDNHVHHGNPLGIDV
                     RQVAWKRILDVNDRALRNVIVGLGGRMDGVPREGGFLITSASEIMAALCLAEDLMDLK
                     QRLGRMLVALTADGKPVTADALGVTGAMTALLRDAIHPNLVQTMEGTPALVHGGPFAN
                     IAHGCNSVLATRLALKLGDICLTEAGFATDLGAEKFFDIKCRLAGLKPDAAMIVATAR
                     ALKYHGGVPVPELDRENVAALTTGLENLEAHVEAVRQFKVPVLIGLNRYPSDTEREYA
                     AVRERCRRLGVEVHVADVFGRGGEGGEELARGLQKLLATERSGFQPLYALDDPITVKL
                     DTIARRIYGADGVEYPARVERQIAQAESLGYGKLPICVAKTQRSLSDDPSRLNRPRGF
                     TITVNEVRISAGAGFLVAITGDITTMPGLPRRPNAERVDVTPDGAITGLF"
     gene            complement(2682..3104)
                     /locus_tag="VNU03_10375"
     CDS             complement(2682..3104)
                     /locus_tag="VNU03_10375"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWP74914.1"
                     /translation="MPSVTHLRVRYKDTDTMSVVYYGNYLTYFEVGRVEYLREYGWPM
                     SRVNERVHLPVVEAFVKYVKPARLDELLEITSRVSERRRASFRFAYEIHNEARDLIAT
                     GHTLHACWNPATAQMIPIPDWLKEIMPDADLDPATARR"
BASE COUNT          537 a         1282 c         1262 g          579 t
ORIGIN      
        1 acgggcagcc agcgcatcag cgggcctcct cctcggcggc caccacgtcg ggcgcgacct
       61 gctcgcccgc gaccacccgg atgaagacct cctcgaggct catgcccacc tggtgcagct
      121 cctggagggc ccagttcttg ccggtgacga ggcggaccag gtcgcctcgc acgtcgcggt
      181 cgcgctccga ctcgaccacg aaccccaccg agccgtcgcg cgaggccagg ggctcgaccg
      241 ccaccacccc cggcaccgcc cgcagggcgc cggccacgtc gcccgacgcg ttcgtcaccc
      301 ggacctcgat gcgggcggtc gggaacacct gctgcaccag ctccgactcg gtcccctggg
      361 cgacgatgcg gccgtggttg atgatgacga tgccgtcgca caccatcgac acctcgggca
      421 ggatgtgggt cgagaggatt acggtgtgct ggccggccag cgagcggatc agcgcgcgga
      481 tttccgagat ctgcttcgga tcgagcccga tggtgggctc gtcgaggatc aacacctccg
      541 ggtcgcccag cagggcctgg gccagcccga cccgctgccg gtagcccttg gagagcttgc
      601 cgatcagccg gttctgcatg tcggtgatga agcagcgctc catcacgtcg gcgacccggc
      661 cgcggcgctc cgcgcgtccc actcccttga tctcggccac gaagtccaga tacgaggaga
      721 ccgagagatc gggatagagc ggggcgctct ccgggagata gccgatgcgc cggcggacct
      781 cgagggattg cgagaacacg tcgtagccgg ccacccgcgc ggtgccgccg gtcgcgggca
      841 tgaagcacga caggatcttc atggtcgtgg acttgcccgc cccgttgggc ccgagaaagc
      901 ccacgatgcg gcccttgtcc acgctgaacg acacgtcttg gatggccgtg accggcccgt
      961 agcgcttggt gagattgtcg acctggatca tggcagtggc tcggttttcg cccgcgccgc
     1021 ccgaaagtat atcacgcgct caaaacagcc ccgtgatggc gccgtccggc gtgacgtcga
     1081 cgcgctccgc gttggggcgc cgcgggaggc cgggcatcgt ggtgatgtcg ccggtgatcg
     1141 cgaccaggaa gccggccccc gccgagatgc gcacctcgtt gacggtgatg gtgaatccgc
     1201 ggggccggtt gagccgcgac gggtcgtcgg agagcgagcg ctgggtcttg gccacgcaga
     1261 tcggcagctt cccgtagccc aggctctcgg cctgggcgat ctggcgctcg acccgcgccg
     1321 ggtactcgac cccgtccgcc ccgtagatcc gccgggcgat ggtgtcgagc ttcaccgtga
     1381 tggggtcgtc gagggcgtag agcggctgga agcccgagcg ctcggtggcc aggagcttct
     1441 ggaggccgcg ggccagctcc tcgccgccct ccccgccccg tccgaacacg tcggccacgt
     1501 gcacctcgac cccgagccgc cggcagcgct cgcgcaccgc cgcgtactcc cgctcggtgt
     1561 ccgacggata gcggttcagc ccgatcagca ccggcacctt gaactgccgc accgcctcca
     1621 cgtgggcctc caggttctcg agcccggtgg tgagcgcggc cacgttctcc cgatccagct
     1681 ccggcaccgg cacgccgccg tgatacttga gcgcccgcgc ggtggccacg atcatcgccg
     1741 cgtcgggctt gaggccggcc aggcggcact tgatgtcgaa gaacttctcc gcccccaggt
     1801 cggtggcgaa cccggcctcg gtcagacaga tgtcgccgag cttcagggcc agccgcgtcg
     1861 ccagcaccga gttgcacccg tgcgcgatgt tggcgaacgg gccgccgtgc acgagcgcgg
     1921 gggtgccctc catggtctgc accaggttcg ggtggatcgc gtcgcgcagc agcgcggtca
     1981 tggccccggt gacgccgagg gcgtcggcgg tcaccggctt gccgtcggcg gtcagcgcca
     2041 ccagcatgcg gccgagccgc tgcttgaggt ccatcaggtc ctcggcgagg cagagcgcgg
     2101 ccatgatctc ggacgccgag gtgatcagga acccgccctc gcgcggcacc ccgtccatcc
     2161 ggcccccgag cccgacgatg acattgcgaa gcgcgcggtc gttgacgtcg aggatccgct
     2221 tccacgccac ctggcggacg tcgatgccga gcgggttgcc gtggtgcacg tggttgtcga
     2281 gcgccgccgc gagcaggttg tgcgccgagg tgatcgcgtg gaagtcaccg gtgaagtgca
     2341 ggttgatctc gtccatcggg accacctgcg accgaccccc gccggtgccg ccgcccttca
     2401 tgccgagcgt cggcccgatc gacggctcgc gcagggcgat gaccggccgg ccgcccaccc
     2461 gccacagcgc ctggccgagg ccgatggtca tggtggtctt gccgtcgccc gccggggtgg
     2521 gcgtgatcga ggagaccacc accagcttgc cgtcgggctg gcccgcgcgg gccgcgagcg
     2581 cgcccacgcg gaccttcgcc ttgtcgtggc cgtacgggat ccactcgtcg tcgcccaggc
     2641 ccagatcgcg cgcgatctgg gcgatcggac ggggcttccg gtcagcgtcg ggccgtggcg
     2701 gggtcgaggt cggcatcggg catgatctcc ttcagccagt cgggaatggg aatcatctgg
     2761 gcggtggccg ggttccagca cgcgtggagc gtgtggccgg tagcgatcag gtcccgggcc
     2821 tcgttgtgga tctcgtaggc gaagcggaag ctggcgcgcc gccgctcgct cacccgcgag
     2881 gtgatctcga gcagctcgtc gaggcgggcc ggcttgacgt acttgacgaa ggcctccacc
     2941 accggcaggt gcacccgctc gttgacccgc gacatgggcc agccgtactc gcgcaggtac
     3001 tccacccgtc ccacctcgaa gtaggtgaga tagttgccgt aatagacgac cgacatagtg
     3061 tcggtgtcct tgtagcgcac gcgcaagtgg gtgaccgagg gcatcttaga cctgacgggc
     3121 acttataggc gactgctcgc cctccgtcaa gggttccagc gcctcgcggg gcgctttgtg
     3181 ctgaccttgg gctgaacctt ccgtcttgga atcggaagtt gtcgatcttg gcccggacca
     3241 cggcggcctc gaatgcatgc cgcgccccgc gatgccaaat cttcccaccc ggacaccctg
     3301 acagcctccg tactgcatcg gactgggccg cgtccacccc tacttggacc aaggtccact
     3361 aggcggggcc ggggcgaccc ttgcacacta tcggctgcct gcaccccggt tttcggttgg
     3421 cgccgctggc gtcgagaccc cggtgaatct ggcgtgtcaa atccgctgcc gccgcgaggt
     3481 gccccatgga caagcccacc cttcgtctcc tgattcggga aaagttggcc gatgggcgtc
     3541 ttccccacaa tcacattccc cgcatgtggg gcgggcccgg cgcgggggaa atctgcgacg
     3601 gctgcggtga ggtcgtaaca caaacgcaga tgttgatgga ggggctgagt aaggactccg
//
LOCUS       DATMCV010000451         4543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_223075, whole genome shotgun sequence.
ACCESSION   DATMCV010000451 DATMCV010000000
VERSION     DATMCV010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223075"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VNU03_10380"
     CDS             complement(<1..255)
                     /locus_tag="VNU03_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HWP74915.1"
                     /translation="MTPPALNHRLTTLDATFLYTEKPVQPMHVGGSMLYEGHLSLDVL
                     VRALECRLHLLPRYRQKVVFPPFAAAHPTWEDDPAFDMRHH"
     gene            complement(282..1007)
                     /locus_tag="VNU03_10385"
     CDS             complement(282..1007)
                     /locus_tag="VNU03_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP74916.1"
                     /translation="MPIWRESLAGLDWLALRTSPVFYGCGVPRGDGAGVVLVPGFLGT
                     DWYLLELHGWLGRLGYRSHLSHIGRNAECLDLLSGRLLETVERAREATGRPVHLIGHS
                     LGGMLTRSLATRRPDLVASVVTLGSPFRGVRSHPLVLLISERVRARVRREDRPDCYTG
                     YCRCEAVTGLEAAFPASVPQLAVYTRTDGIVDWRMCLGDDPASNVEVTGTHVGLVANP
                     AVYRAVAHHLAGASRPAPAASAP"
     gene            1128..2132
                     /locus_tag="VNU03_10390"
     CDS             1128..2132
                     /locus_tag="VNU03_10390"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWP74917.1"
                     /translation="MIAAVLRGPRALALTTVPTPSPGPLDAVVRVKAAGLCGTDYRIW
                     TGERPVQYPRVMGHEFVGTVAAVGADVRHLTVGQAVAVEPNYSCGTCPLCREGNRNLC
                     LSRVAVGIDVDGGFAEQATVPARCCWPAPAGLADERLLLAEPLAVVVRAVGRAAPEAG
                     ESAAVLGLGSLGLLAVQTLRARGVRVLAVARSTRRLGLARELGAEAVALSGADDDTAA
                     ARAFSGREGVDLVIETAGTALAVEQATNLCRPGGRVVLTGLPHEPTSLNFFWVVRREL
                     RIIGSMIYQDEFPEAIRLLATGSVRVERLLSQTFPLARIDEAFEAHRSPDSIKVAVIP
                     "
     gene            2372..3946
                     /locus_tag="VNU03_10395"
     CDS             2372..3946
                     /locus_tag="VNU03_10395"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HWP74918.1"
                     /translation="MTLPASPLTPDQQILHALNRLGYGPRPGDVDRVRRIGLAAYVDE
                     QLSPSGLADPAVEQALPAYPVLARSAADLVREYPQPTPQVRQQLASGQMSRQAAAEIY
                     PPERRPAVITAQMQAARVTRAVLSERQLEEVLVDFWFNHFNVYAQKGAVRWMLPAYER
                     EAIRPHVLGRFRDLVLATARHPAMLFYLDNWLSTRADLVVPRGPNAGRRAGLNENYAR
                     ELMELHTLGVDGGYTQQDVVEVARCFTGWTIDRPQQGGGFVFRTAAHDRGEKRVLGQV
                     IPAGGGREDGERVIDILARHPATARFIATKLTRRFVSDDPPPALVERAAATFQRTDGS
                     IRAVLVTILTSPEFWSAEAYRAKIKTPLEVVASAVRTLDGRLVTVADGRDGLGDGGMV
                     LAREVARLGEPLYEMQPPTGYPDRAESWVNTGALLGRMNFALGLAHNRLRGARVDLAG
                     AVAGADRGQPAQVLDRLLAAVLHGEASPATRAVLAAELESPEITRVTPYDRVAKDTDV
                     EKLAALVLGSPEFQRR"
     gene            3954..>4543
                     /locus_tag="VNU03_10400"
     CDS             3954..>4543
                     /locus_tag="VNU03_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74919.1"
                     /translation="MSGCTRRSFVKGGALALVALGAAPRFLVRTALAQERAGRPKVLI
                     AIFQRGAVDGLSMVVPHGDPDYYGVRGSIAIARPAPGVADTTVDLDGFFGLHPSLAPL
                     RPLWDDRRLAVVHACGSTDTTRSHFDAQDYMESGTPGVKSTPDGWLARGLAGMPAREP
                     ASPFRAVSLGAALPRILRGDVGAVAMSSVSDFDVKEG"
BASE COUNT          581 a         1643 c         1710 g          609 t
ORIGIN      
        1 gtggtggcgc atgtcgaagg ccggatcgtc ctcccaggtg ggatgcgccg ccgcgaaggg
       61 cgggaacacg accttctggc gatagcgcgg gagcaggtgg aggcggcact cgagggcgcg
      121 caccagcacg tcgagcgaca gatgcccctc gtacagcatg gagccgccca cgtgcatcgg
      181 ctggacgggc ttctcggtgt agaggaaggt cgcgtcgaga gtcgtgagcc gatggttgag
      241 cgccggcggc gtcatgctct ggcggggctc actcggcccg gctacggagc ggacgcagcc
      301 ggagccggcc gggacgcccc ggccagatga tgcgcgaccg cccggtagac ggccggattg
      361 gcgaccaggc ccacgtgcgt gcccgtcacc tcgacgttgc tcgccgggtc gtcgcccagg
      421 cacatgcgcc agtccacgat gccgtcggtc cgcgtgtaca ccgcgagctg gggcacggag
      481 gcggggaatg ccgcctcgag cccggtgacc gcctcgcagc ggcagtaccc ggtgtagcag
      541 tcgggccggt cctcgcgccg cacccgcgcg cgcacgcgct cggagatcag cagcaccagg
      601 gggtgcgagc gcaccccacg gaacggggag ccgagcgtca ccaccgacgc gaccaggtcg
      661 ggtcgccggg tcgccagcga gcgggtgagc atgccgccca ggctgtggcc gatcaggtgc
      721 acgggccgtc cggtggcttc ccgcgcgcgc tcgacggtct cgagcaggcg ccccgagagc
      781 aggtcgaggc actccgcgtt ccgcccgatg tgcgagaggt gggaccggta gccgagccgg
      841 cccagccagc cgtggagctc gagaaggtac cagtcggtgc cgagaaagcc cggcaccagc
      901 accacgcccg cgccgtcgcc gcggggcacg ccgcagccgt agaagacggg cgacgtccgg
      961 agcgcgagcc agtcgagacc ggccaggctc tctcgccaga tcggcacgga ggccggccgc
     1021 tgatccggcg cgaaggaggg tgacggcgcc gttgccatgt gcggccatgc tagcacggcc
     1081 gcccgccgcc ttgcctcgac agcgccgggt ccgctaccat agcggacatg atcgccgcgg
     1141 tcctgcgggg ccctcgcgcg ctggcgctca ccaccgtgcc gacaccctcc cccgggcccc
     1201 tcgacgcggt ggtgcgggtc aaggccgccg gtctctgcgg cacggactac cggatctgga
     1261 ccggcgaacg gcccgtgcag tatccgcgcg tcatggggca cgagttcgtc ggcacggttg
     1321 ccgcggtcgg ggcggacgtg cgacatctca cggtcggcca ggcggttgcg gtggagccga
     1381 actactcctg cggcacctgt ccgctctgcc gcgagggcaa ccggaacctg tgcctgtcgc
     1441 gcgtcgcggt ggggatcgac gtggacggcg ggttcgccga gcaggcgacc gtgcccgcgc
     1501 gctgctgctg gcccgcgccc gccggcctgg ccgacgagcg cctgctcctg gccgagcccc
     1561 tggcggtggt ggtgcgtgcg gtcggccgcg ccgcgccgga ggcgggcgag agcgcggcgg
     1621 tgctcggact gggctcgctc ggcctgctcg cggtgcagac gcttcgcgcg cgcggcgtcc
     1681 gggtgctcgc ggtggcgcgc tcgacgcgac gcctcgggct ggcccgcgag ctgggggccg
     1741 aggcggtggc gctctcgggc gccgacgacg acaccgcggc cgcgcgggcc ttctcgggcc
     1801 gcgagggcgt cgatctcgtc atcgagaccg ccgggaccgc gctcgcggtg gagcaggcga
     1861 ccaacctctg ccgccccggg ggccgggtgg tcctgaccgg cctgccccac gagccgacct
     1921 cgctcaactt cttctgggtg gtgcggcgcg agctgcgcat catcggctcg atgatctacc
     1981 aggacgagtt ccccgaggcg atccggctcc tcgcgaccgg ctcggtgcgg gtggagcggc
     2041 tcctctcgca gaccttcccg ctggcccgca tcgacgaggc cttcgaggcg caccgctcac
     2101 cggattcgat caaggtggcg gtgatcccct agacggggca gggtaggccg gccgggccgg
     2161 tcgagcgcct tttcactccc tgtaaacgtt aagatacagg gagacgtctg ctcctcagga
     2221 gggcggcatg gggctctgga gacgaggtgt gctggcgatc ctgttaggcc ttccgaccgc
     2281 cgcctgctcc tcggcgtcgc ctccggctgc ggcacccacc acggcgggcc acccggtccc
     2341 ggtcgtcgcc ccggcccgcg ccggggctcg catgacgctg ccggcttcgc cgctcacccc
     2401 cgatcagcag atcctccacg cgttgaaccg gctcgggtac gggcctcggc cgggtgacgt
     2461 ggatcgggtg aggcgcatcg ggctcgcggc ctacgtcgac gagcagctca gcccgagcgg
     2521 cctcgccgac cccgcggtcg agcaggccct gccggcctac ccggtgctcg cacggagcgc
     2581 ggcggatctc gtgcgcgagt atccgcagcc gaccccccag gtgcgccagc agctggcctc
     2641 cgggcagatg agccgtcagg ccgcggccga gatctacccg ccggagcggc ggcccgcggt
     2701 gatcacggcc cagatgcagg cggcacgggt cacgcgcgcg gtgctgagcg agcgccagct
     2761 cgaggaagtg ctggtcgatt tctggttcaa ccacttcaac gtgtacgccc agaagggcgc
     2821 ggtgcggtgg atgctgcccg cctacgagcg ggaggcgatc cgtccccacg tcctcgggcg
     2881 ctttcgcgac ctggtgctgg ccaccgcgcg gcatcccgcg atgctgttct acctcgacaa
     2941 ctggctcagc acccgcgccg atctggtggt gccccgcggg cccaacgcgg gccggcgcgc
     3001 cggcctcaac gagaactacg cgcgcgagtt gatggagctg cacaccctcg gtgtggacgg
     3061 cggctatacc cagcaggacg tggtggaggt ggcgcgctgc ttcaccggct ggaccatcga
     3121 ccggccgcag cagggcgggg gcttcgtctt ccgcaccgcg gcccacgacc gtggcgagaa
     3181 gcgcgtgctc ggccaggtga tcccggccgg aggcggtcgg gaggacggcg agcgggtgat
     3241 cgacatcctg gcgcgccatc ccgcgaccgc gcgcttcatc gcgaccaagc tcacgcggcg
     3301 cttcgtgagc gacgatccgc cccccgcgct ggtggagcgc gcggcggcca cgttccagcg
     3361 caccgacggg agcatccggg cggtgctggt gacgatcctc acgtcgcccg agttctggtc
     3421 ggccgaggcc taccgggcta agatcaagac ccccctcgag gtggtggcga gcgcggtgcg
     3481 cacgctcgac ggccggctcg tgacggtcgc ggacggccgc gacgggctcg gcgacggcgg
     3541 gatggtgctg gcccgcgagg tggcgcggct cggcgagccc ctctacgaga tgcagccgcc
     3601 caccggctac ccggaccgcg ccgagtcgtg ggtcaacacc ggcgcgctgc tcgggcggat
     3661 gaacttcgcg ctcgggctcg cccacaaccg cctgcgcggc gcgcgggtcg acctggccgg
     3721 ggccgtcgcg ggcgcggacc gtggccagcc cgcccaggtg ctggatcgcc tgctcgccgc
     3781 ggtgctgcac ggggaggctt cgccggcgac ccgcgcggtg ctcgcggccg agctcgagag
     3841 tccggagatc acccgagtca ccccctacga ccgggtggcc aaggacaccg acgtggagaa
     3901 gctggccgcg ctcgtcctgg gctcgcccga attccagcgg aggtaagacg ctcatgtccg
     3961 ggtgcacgcg gcggtcgttc gtgaagggcg gggccctcgc gctggttgcg ctcggcgcgg
     4021 ccccgcggtt cctggtgcgc accgcgctcg cgcaggagcg ggccgggcgg cccaaggtgc
     4081 tcatcgcgat cttccagcgc ggcgcggtcg acggcctcag catggtggtg ccgcacggtg
     4141 atcccgacta ctacggggtc cgcggctcca tcgcgatcgc gcggccggct ccgggcgtcg
     4201 ccgacaccac cgtcgacctc gacggcttct tcggcctgca cccgtcgctc gctcccctca
     4261 ggccgctgtg ggacgaccgg cggctcgcgg tggtccacgc gtgcggctcg accgatacca
     4321 cccgctcgca cttcgacgcg caggattaca tggagtcggg aacgcccggc gtgaagagca
     4381 cgcccgacgg ctggctggcc cgcggcctcg ccgggatgcc ggcccgcgag ccggcctcgc
     4441 cgttccgcgc ggtgtcgctc ggggcggcgc tcccgcgcat cctccgcggc gacgtgggcg
     4501 cggtcgcgat gagcagcgtg agcgatttcg acgtgaagga ggg
//
LOCUS       DATMCV010000452         3836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_686652, whole genome shotgun sequence.
ACCESSION   DATMCV010000452 DATMCV010000000
VERSION     DATMCV010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686652"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VNU03_10405"
     CDS             <1..1485
                     /locus_tag="VNU03_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWP74920.1"
                     /translation="LIVGLPGPDATDDDVRLLREIHAGGLMLYRRNFETPERFVRFIR
                     RLEEGVGRRLMITTDHEGGRIIMLNQGVTIFPDNLTAGTAGEEAMVARQGQIEGRELR
                     RLGVDINFGPCLDVLTERYSPNIGIRSYGKDPELVARFGAARIRGMQSTGLSACAKHF
                     PGKGHAPVDAHLGLPVIDSDWGDMHAVHLVPFMAAMEAGVDCVMTSHPLYPNLDPAER
                     MPATFSKLIVEDYLRGQVGYRGVIISDDLEMGAIAALCPMGEAAVRTAAAGHDLLLVC
                     HTAPAQRAAYEAVVAAYRAKTLPLRALERSMARLDALDARRPERFTGGDPRAEKDGEP
                     LARAMAARAVMVVAPPAPGWKPALNGRVAAIFPRLSALAARITIEPVMQDEPRYLREA
                     MAPHGVRPEVTVVGVEPTAEEIEQARAAAAAADATVLFLYDAHLYKSNQALLEACQAG
                     AKRLAVVLMRDPWDAELLVPGVAAVTAYGWRRSQLDAVMARLLT"
     gene            complement(1505..1927)
                     /locus_tag="VNU03_10410"
     CDS             complement(1505..1927)
                     /locus_tag="VNU03_10410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWP74921.1"
                     /translation="MSDELLERVRARARDNAFWRYLGVEVVEASEGRVRLVVRARDEI
                     RNAPGAPVHGGVFSALVDMAVGGALSTMHDAAAGGVGQVTLDMNVSFIGAGSGDVYAE
                     GRILRRGRSVAFGEATITDAAGKLLAVGRATYMIVAPK"
     gene            complement(1924..2931)
                     /locus_tag="VNU03_10415"
     CDS             complement(1924..2931)
                     /locus_tag="VNU03_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWP74922.1"
                     /translation="MTALAASPGGDHGVRLFAIAVSLVVSLGLLAAKFYAYRLTGSTA
                     ILSDALESIVNVVAAVFAIGALIFAGRPADRNHPYGHGKMEFLSAAFEGGLIAFAAVL
                     IVYEVIQSLLGGVTLRSLDLGVAVVLGAGVVNLLLGLYLLRTGRTYESLALVADGRHV
                     LSDFYTSAGIVVGLLLVRATGIAWLDPVVAAVVALNLMWTGVRLVRQAAAGLLDEEDT
                     GLLVRLLGVLQPHLGQGVIRVHHLRAIRAGRFHHVDAHLVVPEFWSVERSHELAEDLA
                     ERVMKELGAEGEMTLHTDPCHRIYCRMCDLEDCVIRRDLFTGRPPLTLEEAVQPDMPR
                     P"
     gene            3019..3717
                     /locus_tag="VNU03_10420"
     CDS             3019..3717
                     /locus_tag="VNU03_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWP74923.1"
                     /translation="MIVKRPAAERGHFDHGWLDTSHTFSFSDYHDPSHMGFRSLRVIN
                     EDRVAPGKGFGTHSHRDMEIVTYVLEGALEHADSMGTRSVIVPGDVQRMSAGTGVTHS
                     EFNGKRDRPVHFLQIWLLPDRRGLAPGYEQKAIPAAEERGALRLVASRDGRDGSVTIH
                     QDADLYASRLEPGERVKHALAGGRHAWLQMVSGRIELNGTALAAGDGAAVSQESALEM
                     VAVEPAHFLLFDLA"
BASE COUNT          577 a         1411 c         1347 g          501 t
ORIGIN      
        1 ctcatcgtgg ggctccccgg gcccgacgcc accgacgacg acgtgcggct gctccgggag
       61 atccacgccg gcggtctgat gctctaccgg cgcaacttcg agacccccga gcgcttcgtg
      121 cgcttcatcc ggcggctcga ggaaggcgtc ggacggcggc tgatgatcac caccgatcac
      181 gagggcggcc gcatcatcat gctgaaccag ggcgtcacga tcttccccga caacctcacc
      241 gccggcaccg cgggcgagga ggcgatggtg gcccgccagg ggcagatcga gggacgcgag
      301 ctgcgccgcc tcggggtcga catcaacttt ggcccctgcc tcgacgtgct caccgagcgc
      361 tacagcccga acatcggcat ccgctcctac ggcaaggacc cggagctggt cgcccgcttc
      421 ggcgccgcgc gcatccgcgg catgcagtcg accgggctct cggcctgcgc caagcacttc
      481 ccgggcaagg gccacgcgcc ggtcgacgcg cacctgggcc tgccggtgat cgactcggac
      541 tggggcgaca tgcacgcggt ccacctggtt ccgttcatgg ccgcgatgga ggcgggagtg
      601 gactgcgtca tgacctcgca cccgctctac ccgaacctcg acccggccga gcgcatgccg
      661 gccacgttct cgaagctgat cgtggaggac tacctgcgcg gccaggtggg ctaccgcggc
      721 gtcatcatct ccgacgatct cgagatgggc gcgatcgccg cgctgtgccc catgggcgag
      781 gccgcggtgc gcaccgcggc ggccggccac gacctgctgc tggtctgcca caccgcgccg
      841 gcccagcgcg cggcctacga ggcggtggtc gcggcctacc gcgccaagac cctgcccctg
      901 cgcgcgctcg agcggagcat ggcgcgcctc gacgcgctcg acgcccggcg gcctgagcgc
      961 ttcaccgggg gcgacccccg cgcggagaag gacggcgagc cgctggcccg tgcgatggcc
     1021 gcgcgcgcgg tgatggtggt ggcgccgccc gctcccgggt ggaagcccgc cctcaacggg
     1081 cgtgtggccg cgatcttccc gcggctctcc gcgctggccg cgcgcatcac catcgagccc
     1141 gtcatgcagg acgagccgcg ctacctgcgc gaggccatgg cgccgcacgg ggtccggccc
     1201 gaggtgacgg tggtgggggt ggagccgacc gcggaggaga tcgagcaggc gcgcgccgcc
     1261 gcggccgccg ccgacgcgac ggtgctcttc ctctacgacg cgcatctcta caagtcgaac
     1321 caggcgctgc tggaggcctg ccaggcgggc gcgaagcggc tggcggtggt gctgatgcgc
     1381 gacccgtggg acgcggagct gctggtcccg ggcgtggccg cggtcaccgc gtacggttgg
     1441 cggcgatctc agctcgacgc ggtgatggcg cggctgctga cgtaaagcgc gcccggggag
     1501 agggttactt gggggccacg atcatgtagg ttgcgcgccc caccgcgagc agcttgccgg
     1561 ccgcgtcggt gatggtggcc tccccgaacg ccaccgaccg cccgcgtcgc aggatgcgcc
     1621 cctccgcgta gacgtcgccg ctgcccgccc cgatgaagct cacgttcatg tcgagcgtga
     1681 cctgtccgac ccctcccgcg gcggcatcgt gcatggtgga gagcgcgccg cccaccgcca
     1741 tgtcgaccag ggccgagaac accccgccgt gcaccggcgc gccgggcgcg ttgcggatct
     1801 cgtcgcgcgc ccggacgacg agccgtaccc ggccctcgct cgcctcgacc acctcgacgc
     1861 ccaggtaccg ccagaaggca ttgtcgcgcg cgcgggcgcg cacgcgctcc agcagctcgt
     1921 cgctcatggt cggggcatgt cgggttgaac cgcctcctcc agggtcagcg ggggccggcc
     1981 cgtgaagagg tcgcggcgga tcacacagtc ctccagatcg cacatccggc agtagatgcg
     2041 atggcaggga tcggtgtgga gcgtcatctc gccctccgcg ccgagctcct tcatcacgcg
     2101 ctcggccagg tcctcggcca gctcgtgcga gcgctccacc gaccagaact cgggcaccac
     2161 caggtgggcg tccacgtggt ggaagcgacc ggcccggatc gcgcgcaggt ggtggacgcg
     2221 gatcactccc tgccccaggt ggggctgcag gactccgagc aggcggacga ggagaccggt
     2281 gtcctcctcg tccaggagcc cggccgcggc ctggcgcacc aggcgcaccc cggtccacat
     2341 gaggttcagc gccaccaccg ccgccaccac cggatcgagc caggcgatgc cggtggcgcg
     2401 gaccaggagg aggccaacca cgatccccgc gctggtgtag aagtcgctca ggacgtgccg
     2461 cccgtcggcg acgagggcca gcgactcgta ggtgcgcccg gtgcgcagga ggtagagccc
     2521 gaggagcagg ttcaccaccc ccgcccccag caccaccgcc acccccaggt cgagcgatcg
     2581 cagcgtgacg cccccgagca ggctctggat gacctcgtag acgatcagca ccgcggcgaa
     2641 cgcgatgagg ccgccctcga aggccgcgct caggaactcc atcttcccgt gcccgtacgg
     2701 gtggttgcga tccgcggggc gacccgcgaa gatgagcgct ccgatggcga acaccgcggc
     2761 caccacgttg acgatggact cgagcgcgtc ggagaggatc gcggtcgagc cggtcagccg
     2821 gtaggcgtag aacttcgcgg cgaggagccc gagcgacacc accagggaga ccgcgatggc
     2881 gaagagccgg acgccgtgat caccgccggg cgacgccgcg agagccgtca tggtcagatg
     2941 atacccgcgc cggaaccgat ttgaaccggg gcgtatcata agggcgtaag gtaaccctca
     3001 ctcgaacagg agcgcgcgat gatcgtcaag cgtcccgccg ccgagcgcgg ccacttcgac
     3061 cacggctggc tcgacacctc gcacaccttc tcattctccg actaccacga cccctcccac
     3121 atgggcttcc gctcgctccg ggtcatcaac gaggaccggg tcgcgccggg caagggcttc
     3181 ggcacccact cgcaccggga catggagatc gtcacctacg tgctggaggg cgccctcgag
     3241 cacgccgaca gcatgggcac ccggtcggtg atcgtgccgg gcgacgtcca gcgcatgagc
     3301 gcgggcaccg gcgtcaccca cagcgagttc aacggcaagc gcgaccggcc ggtgcacttc
     3361 ctccagatct ggctcctgcc cgaccgccgc gggctcgccc ccggatacga gcagaaggcg
     3421 atcccggcag cggaggagcg gggcgcgctg cggctggtgg cctcccgcga cgggcgcgac
     3481 ggcagcgtga ccatccacca ggacgccgat ctctacgcct cgcgcctcga gccgggcgag
     3541 cgcgtgaagc acgccctcgc gggcgggcgg cacgcgtggc tacagatggt gagcgggcgg
     3601 atcgagctga acggcaccgc gctggccgcg ggcgacggcg cggcggtcag ccaggagagc
     3661 gcgctcgaga tggtggcggt cgagcccgcc cacttcctgc tcttcgacct ggcctagctc
     3721 cccacccctc accctgccct ctccccctca ccctgccctc tcccctaagg ggagagggtt
     3781 agggtgaggg gtggttaggc ctggccgagc tgctgctgaa cgaactgctt ggcgtc
//
LOCUS       DATMCV010000453         2508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_890843, whole genome shotgun sequence.
ACCESSION   DATMCV010000453 DATMCV010000000
VERSION     DATMCV010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890843"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..918)
                     /locus_tag="VNU03_10425"
     CDS             complement(52..918)
                     /locus_tag="VNU03_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWP74924.1"
                     /translation="MSTLVGLDDLKAHVGRRESADDVVTAAPANLLRLTFGRDEPELG
                     PGDPLPPGWQCLYFLPRFAPDALRPDGSPLDTGVIPPMPLPRRMFAGERFRFHRPLRI
                     GETIRRETELADISTKTGGTGTLVFATVVQRIFTADGLAIEEERRTVFREEVRAGERN
                     QAPRREPAPDDVPWRRRITPDPVLLFRFSALTFNSHRIHYDRPWAVEREGYAGLVVHG
                     PLTSTLLIDLARDHTRGRALATYETQARAPLFDTAAFELRGRPSADGGAVELWAVTPD
                     GTVAMSARAELA"
     gene            complement(926..2317)
                     /locus_tag="VNU03_10430"
     CDS             complement(926..2317)
                     /locus_tag="VNU03_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP74925.1"
                     /translation="MTADELCYAGIRELGGLFRRGALSPVDYATDLLGRVERLDKSLN
                     SYVTVTAERALADARAAEAAIRRGDAGPLAGIPIAYKDLYATKGIRTTAGSAVLGDWV
                     PDFDCTCVTRLQQAGMVMLGKLITHEFAFGIQFPGHRFPPARNPWNVDHIPGGSSSGS
                     GAALAAGLTVGSLGSDTGGSIRGPAAFCGIAGLKPTYGRVSRAGVVTLSWTLDHTGPM
                     ARSAEDCAFMLQALAGHDPADPASAREPVPDYLAGLEGGVKGLRVGVVRDYFFEQVEP
                     ETVAAFERALAELGRLGATVRDVTIPSIHATPSFMVIMLTEAFSYHERDLREHPELYG
                     DVLREKLMAGGLFRADEYVQAQRLRAQIREDTARALESVDVLITPTALGPAPAFKVVL
                     DPDFPFAKSNMGPFNITGLPTLAIPTGFSSSGLPLSMQISGRPFDEATVLRTGHAYQR
                     ATEWHRRRPPVPA"
     gene            complement(2314..>2508)
                     /locus_tag="VNU03_10435"
     CDS             complement(2314..>2508)
                     /locus_tag="VNU03_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74926.1"
                     /translation="PPEVTLDEVKERARLAGLPIPAGRLPMVRAFLVNALRPIRALDT
                     RTVRTVEPAVTFDAKGGALR"
BASE COUNT          345 a          847 c          982 g          334 t
ORIGIN      
        1 gagttgctcg tcggccatcg tggctgatcc tttctgtcgt cggagagatc gtcaggcgag
       61 ctcggcgcgg gcgctcatgg ccaccgtgcc gtccggagtc accgcccaga gctcgaccgc
      121 gcccccgtcc gcggacgggc ggccgcgcag ctcgaaggcg gcggtgtcga agagtggcgc
      181 gcgggcctgg gtctcgtagg tggcgagggc gcgcccgcgg gtgtggtcgc gggcgaggtc
      241 gatcaggagg gtggaggtga gcgggccgtg gactaccaaa ccggcgtatc cttcccgctc
      301 caccgcccag ggccgatcgt agtggatgcg gtggctgttg aacgtcagcg cggagaagcg
      361 gaagagcagc accggatccg gcgtgatgcg ccgccgccac ggcacgtcgt ccggggcggg
      421 ctcgcggcgc ggagcctggt tgcgctcgcc cgcccgcacc tcctcgcgga agacggtccg
      481 ccgctcctcc tcgatcgcga gcccgtcggc ggtgaagatg cgctggacca cggtggcgaa
      541 caccagggtc ccggtgccgc cggtcttggt ggagatgtcg gccagctcgg tctcgcggcg
      601 aatcgtctcg ccgatgcgga gcggccggtg gaagcggaag cgctcgcccg cgaacatacg
      661 gcggggcagc ggcatcggcg ggatcacccc ggtgtcgagc gggctgccgt cgggccgcag
      721 cgcgtccggg gcgaagcgcg gcaggaagta gaggcactgc cagccgggcg gcagcggatc
      781 gcccggaccc agctcgggct cgtcgcggcc gaaggtgagc cggaggagat tcgcgggcgc
      841 cgcggtgacg acgtcatcgg cgctctcgcg ccgtccgacg tgtgccttca gatcgtcgag
      901 accgaccagg gtgctcatgg ggtcgtcatg cggggaccgg cggccggcgg cggtgccact
      961 cggtggcccg ctggtaggcg tggccggtcc gcagcacggt cgcctcgtcg aagggccggc
     1021 cggagatctg catcgagagc ggcaggccgg aggacgagaa gccggtcggg atcgccaggg
     1081 tcggcagccc ggtgatgttg aacgggccca tgttcgactt ggcgaacggg aagtcggggt
     1141 cgagcaccac cttgaaggcg ggcgcgggac cgagcgcggt gggcgtgatc aggacgtcca
     1201 ccgactcgag cgcgcgggcg gtgtcctcgc ggatctgggc gcgcaggcgc tgggcctgca
     1261 cgtactcgtc ggcgcggaag agcccgcccg ccatgagctt ctcgcgcagc acgtcgccgt
     1321 acagctccgg gtgctcgcgc aggtcgcgct catgatacga gaaggcctcg gtgagcatga
     1381 tgaccatgaa cgacggcgtc gcgtggatgc tggggatcgt gacgtcgcgg acggtggctc
     1441 ccagccgtcc cagctcggcg agcgcccgct cgaaggcggc caccgtctcc ggctcgacct
     1501 gctcgaagaa gtagtcgcgc accaccccga cccggagccc cttcaccccg ccctcgagac
     1561 ccgccaggta gtcgggcacc ggctcccgcg cggaggccgg atcggccgga tcgtggccgg
     1621 ccagcgcctg cagcatgaac gcgcagtcct cggccgagcg ggccatgggc ccggtgtggt
     1681 cgagcgtcca cgagagcgtg accactcccg cgcggctgac acggccgtag gtgggcttga
     1741 gcccggcgat gccgcagaag gcagccggac cccggatcga gccgccggtg tcggagccga
     1801 gcgagcccac cgtgaggccg gctgcgagcg cggctcccga gccgctgctg gagccgcccg
     1861 ggatgtggtc gacgttccac gggttgcgcg cgggcgggaa gcggtggccg gggaactgga
     1921 tgccgaaggc gaactcgtgg gtgatgagct tgcccagcat caccatgccc gcctgctgga
     1981 gccgggtcac gcaggtgcaa tcgaaatccg ggacccagtc gcccaggacc gccgagcccg
     2041 cggtggtgcg gatgcccttg gtcgcgtaga ggtccttgta ggcgatcggg atgcccgcga
     2101 gggggcccgc gtcgccgcgc cggatggcgg cctccgccgc gcgggcgtcg gccagcgcgc
     2161 gctccgcggt gaccgtcacg taggagttga ggctcttgtc gaggcgctcg acgcgtccga
     2221 gcaggtcggt cgcgtagtcg accggcgaca gcgcgccccg gcggaagagc ccgccgagct
     2281 cccggatgcc cgcgtagcag agctcgtcgg cggtcatctc aacgctccgc ccttcgcgtc
     2341 gaacgtcacc gcgggctcga ccgtccgcac ggtgcgggtg tcgagcgcgc ggatcggccg
     2401 cagcgcgttc accaggaacg cgcgcaccat gggcaggcgg cccgccggaa tgggcaggcc
     2461 ggcgaggcgg gcgcgctcct tcacttcgtc cagcgtgacc tcaggggg
//
LOCUS       DATMCV010000454         5194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_758520, whole genome shotgun sequence.
ACCESSION   DATMCV010000454 DATMCV010000000
VERSION     DATMCV010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758520"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VNU03_10440"
     CDS             complement(<1..189)
                     /locus_tag="VNU03_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HWP74927.1"
                     /translation="MLLFAKMLRVGLVSVPIGPPAPGEEDVVHLVREVDAESRRLFGR
                     ALAVREVDAGSCNGCEIEI"
     gene            complement(252..1280)
                     /locus_tag="VNU03_10445"
     CDS             complement(252..1280)
                     /locus_tag="VNU03_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HWP74928.1"
                     /translation="MATHQAVLIPGDGIGPEVTVAVRRILDAAGAPVEWVECRAGLAA
                     LERGQDVLPQETLDAITRLQVALKGPCTTPIGEGFVSINVRLRKELDLYAAVRPVRNL
                     DGVPTRYRDVDLVIIRENTEGLYSGIEATLAPGVVTTTKVATEKACTRIARWAFHYAQ
                     HRGRKKITVFHKANIMKLTDGMFMRCARSVHDQEFSQIGYQEMIIDAGCMGLVQDPTR
                     FDMLLLENLYGDVLSDLCAGLVGGLGVVPGANIGDHGAVFEAVHGSAPDIAGRGVANP
                     LALLMSAVMLLNHLAETRDDAACATAAQRIKDGYNHALAEGCKTRDLGGTLGTAGFAD
                     AVIARLGA"
     gene            complement(1304..2284)
                     /locus_tag="VNU03_10450"
     CDS             complement(1304..2284)
                     /locus_tag="VNU03_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74929.1"
                     /translation="MATTRRSFIKTVAGGTAAAAVGAWAPRTARAATPVKVGCVVLGD
                     FGINAPTMIGIEKGFFKQNDLEAEMVPFKGGPDLLKGVLAGAADIGLTGATDPLVFRE
                     RGTGIRAIATILEKNHFTLTVAPTIKKVEDLKGGSIGVTVVGSTTWVFARMLAKKMGW
                     EPEKDIKIVGVGGIDAQAAALRRGETQGCIFGDAGAVLEAQGVGRIIMRLDEVTPKWI
                     SLVAYTTDDLIKTKRDTIQRTLKAIFQGHKFCRDNPDEAIRIAAKGIGWPEAATRRAY
                     ELVKPLLSVDGRMDLEAMKVMQDTLLDLGVLKQRLPLDQHYTTEFVPVKI"
     gene            complement(2301..3044)
                     /locus_tag="VNU03_10455"
     CDS             complement(2301..3044)
                     /locus_tag="VNU03_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP74930.1"
                     /translation="MGRLGVLGCQALSLAAVLLAWEVAVRTGLADPLFVPTPAAVAGA
                     LGATLRDAWPRLGDTLLKALLGYALAVGLGVAGGLWVGSRRLAHAVAMPYVVAAYGVP
                     KILVLPWIVLICGLGATTAVLTATLFAVFPILLMVAAGTRDVDPTLVTAAVAMGATRS
                     QVSLKVLLPAVLPSVVAGMRVGVVFAMLGVLLAEMFAGTRGMGFLMQRMAMAFKAPDL
                     FAATAIVSVLSIVVVLSLEHLGRRLSRWR"
     gene            complement(3032..3778)
                     /locus_tag="VNU03_10460"
     CDS             complement(3032..3778)
                     /locus_tag="VNU03_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP74931.1"
                     /translation="MKVWAWRLGIAFAGIALWEAVAAPLNPLFYIRPSVLPAALLGMW
                     RVPELPPLSEHVWLTMQEVGAAYLLAVAAGLWLGFLLGLRGTVGRVYEPLLAALYAVP
                     SVVWYPSLMLFFGLGPASKVAFGVLLGFFPIVLSVLAGIRQVPATLVTVARSMGARPW
                     TIFHKVMLPAMASTMVGGLRAGLALSVVGVLVGEILGSRAGVGYVINYAYGLMMTREY
                     AVLAVLVAAGVLALDTAGALVEARVKRWAG"
     gene            complement(3775..4578)
                     /locus_tag="VNU03_10465"
     CDS             complement(3775..4578)
                     /locus_tag="VNU03_10465"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP74932.1"
                     /translation="MPDGVATVALAPIVLDAIGKRYETTSGPVQAVDSVSLTVRREEF
                     VSLLGPSGCGKSTVLGMIGGLVAPDSGRVLLDGAVRTGPSPDKVALVFQDGGLFPWRT
                     ALDNVTFGLELRGVPAAKRREIARGLLEPMGLAGFERKYPRELSGGMRQRVAIARALA
                     LDTPILLMDEPFGALDEQTRLLMGEWLVGIRRRTRKTVVFVTHSLQEAIALSDRIVVM
                     TARPGRIKDVLDVTLPFPRDLNAPEVTELRAKLWEQVREESLKAMGGRP"
     gene            4622..>5194
                     /locus_tag="VNU03_10470"
     CDS             4622..>5194
                     /locus_tag="VNU03_10470"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWP74933.1"
                     /translation="MADAVHLGSGKVRELYALDDERLLLVASDRISTFDVVLPTAIPD
                     KGRVLTGLAAFWFARTRSICPNHLLALRPDGRSTECRRLEMLPIECVVRGYLSGSGWK
                     DYGKTGEVCGHRLPAGLRESDRLPEPIFTPATKAQSGHDENITREQGAAIVGAERFAE
                     AERLSIALYRFAAAYALERGIIIADTKFEFG"
BASE COUNT          798 a         1937 c         1757 g          702 t
ORIGIN      
        1 gatctcgatc tcgcagccgt tgcaggagcc cgcgtccacc tcgcggaccg cgagcgcccg
       61 gccgaagagc cgtcgcgact ccgcgtcgac ctcgcgcacc aggtggacga cgtcctcctc
      121 gcccggcgcc ggcggcccga tcggcacgga aacgagtccc acccttagca tcttcgcgaa
      181 tagcagcatc agccgcctac tatggggccc cggtccgtca ggaccaagga atcagacggc
      241 gggaaggggc gtcaggcgcc gaggcgggcg atgaccgcgt cggcgaaccc ggcggtgccg
      301 agcgtgccgc cgagatcgcg ggtcttgcag ccctcggcga gggcgtggtt gtagccgtcc
      361 ttgatccgct gcgcggccgt cgcgcacgcc gcatcgtcgc gcgtctcggc caggtggttg
      421 agcagcatga ccgcggacat cagcagcgcg agcgggttgg cgaccccgcg gcccgcgatg
      481 tcgggcgcgg agccgtgcac cgcctcgaag accgcgccgt ggtcgccgat gttcgcgccc
      541 ggcaccaccc cgaggccgcc caccaggccc gcgcagagat cgctcagcac gtcgccgtag
      601 aggttctcca gcagcagcat gtcgaagcgg gtggggtcct ggacgagccc catgcagccc
      661 gcgtcgatga tcatctcctg gtagccgatc tgggagaact cctggtcgtg caccgagcgg
      721 gcgcaccgca tgaacatccc gtcggtgagc ttcatgatgt tggccttgtg gaagacggtg
      781 atcttcttcc ggccccggtg ctgcgcgtag tggaaggccc agcgcgcgat gcgcgtgcac
      841 gccttctcgg tggcgacctt ggtggtggtc acgaccccgg gcgccagcgt ggcctcgatg
      901 cccgagtaga ggccctcggt gttctcgcgg atgatgacca ggtccacgtc ccggtaacgg
      961 gtgggcactc cgtcgaggtt gcggaccggc cgcactgccg cgtagaggtc cagctctttg
     1021 cggaggcgca cgttgatgga gacgaagccc tcgccgatcg gcgtggtgca cggccccttc
     1081 agcgcgacct ggaggcgcgt gatcgcgtcc agggtctcct gcggcaggac gtcctggccg
     1141 cgctcgagcg cggcgaggcc cgcgcggcac tccacccact ccacgggggc ccccgccgcg
     1201 tccaggatgc ggcggaccgc gacggtgacc tcggggccga tgccgtcacc gggaatgagc
     1261 acggcctgat gcgtggccat gccgtcatta tccacccggg cgattaaatt ttgaccggaa
     1321 cgaactccgt cgtgtagtgc tgatcgagcg gcaggcgctg cttgaggact cccaggtcga
     1381 gcagggtgtc ctgcatgacc ttcatcgcct ccaggtccat gcgcccgtcc accgacagga
     1441 gcggcttcac cagctcgtag gcccgccggg tggccgcctc cggccagccg atgcccttgg
     1501 ccgcgatccg gatcgcctcg tccggattgt cgcggcagaa cttgtggccc tggaagatgg
     1561 ccttgagcgt ccgctggatg gtgtcccgct tggtcttgat caggtcgtcg gtggtgtagg
     1621 ccaccaggct gatccacttg ggggtcacct cgtccaggcg catgatgatg cgccccactc
     1681 cctgcgcctc gagcaccgct cccgcgtcgc cgaagatgca gccctgggtc tccccgcgcc
     1741 ggagcgcggc ggcctgggcg tcgatgccgc ccaccccgac gatcttgatg tccttctccg
     1801 gctcccagcc catcttcttg gccagcatgc gggcgaacac ccacgtggtc gacccgacca
     1861 cggtgacccc gatggagccg cccttcaggt cctcgacctt cttgatcgtc ggggccaccg
     1921 tcagcgtgaa atggttcttc tcgaggatgg tggcgatcgc ccggatcccg gtgccgcgct
     1981 cgcggaacac gagcgggtcg gtggcgccgg tcagcccgat gtcggccgcg ccggccagca
     2041 cgcccttgag gagatccggc ccgcccttga acgggaccat ctcggcctcg aggtcgttct
     2101 gcttgaagaa ccctttctcg atgccgatca tggtcggggc gttgatgccg aagtcgccca
     2161 gcaccacgca gccgaccttc accggcgtcg ccgcgcgcgc cgtccggggc gcccacgcgc
     2221 ccaccgcggc ggccgcggtg ccgcccgcca ccgtcttgat gaagctccgt cgggtcgtcg
     2281 ccattgatcg gtctcctctg tcagcgccag cgggagagcc ggcggccgag atgctcgaga
     2341 gacagtacca cgacgatgga caggaccgac acgatcgcgg tggccgcgaa gagatcgggc
     2401 gccttgaagg ccatcgccat gcgctgcatg agaaagccca tgccgcgcgt gcccgcgaac
     2461 atctccgcga gcagcacgcc cagcatggcg aagacgacgc ccacccgcat gccggccacc
     2521 accgagggca gcaccgcggg cagcagcacc ttgaggctca cctggctccg ggtcgccccc
     2581 atcgccaccg ccgcggtgac cagggtcgga tcgacgtcgc gggtgccggc ggcgaccatc
     2641 aggaggatcg ggaacaccgc gaagagcgtg gcggtcagca ccgcggtggt ggcgccgagc
     2701 ccgcagatca gcacgatcca gggcagcacc aggatcttgg gcaccccgta ggccgccacc
     2761 acgtagggca tggccaccgc gtgggccagc cgccgcgagc cgacccagag accgcccgcc
     2821 accccgagcc ccaccgccag cgcgtagccg agcagcgcct tcagcagcgt gtcgccgagc
     2881 cggggccatg cgtcccgcag cgtcgcgccg agcgcgcccg ccaccgcggc cggcgtcggc
     2941 acgaagagcg gatcggcgag cccggtgcgc accgccacct cccaggccag gagcaccgcg
     3001 gcgagcgaga gggcctggca gcccaggacg cctagccggc ccatcgcttc acccgcgcct
     3061 ccaccagcgc ccccgcggtg tcgagcgcga gcaccccggc cgccaccagc accgcgagca
     3121 ccgcgtactc gcgcgtcatc atgagcccgt aagcgtagtt gatcacgtag cccaccccgg
     3181 cgcgcgagcc caggatctcc cccaccagca cgcccaccac cgagagggcc agccccgccc
     3241 ggagcccgcc gaccatggtc gaggccattg cgggcagcat gaccttgtgg aagatcgtcc
     3301 acggccgcgc gcccatcgag cgtgcgaccg tcaccagggt ggccggcacc tggcgaatcc
     3361 cggccagcac cgagagcacg atcgggaaga aaccgagcag caccccgaac gcgaccttgg
     3421 acgcgggccc gaggccgaag aagagcatga gcgacgggta ccacaccacg ctcggcaccg
     3481 cgtagagcgc ggccagcagc ggctcgtaga cgcgccccac cgtcccccgc agcccgagca
     3541 ggaagccgag ccagagcccc gccgccaccg ccaggaggta cgcggcgccg acttcctgca
     3601 tcgtcagcca cacgtgctcg gagagcggcg gcagctcggg cacccgccac atcccgagga
     3661 gcgccgcggg caggaccgac gggcgaatat agaagagcgg gttgagcggc gcggccaccg
     3721 cctcccagag cgcgatcccc gcgaacgcga tgccgaggcg ccaggcccac accttcacgg
     3781 gcggccaccc atcgccttga gcgattcctc ccgcacctgc tcccagagct tcgcgcgcag
     3841 ctcggtcacc tcgggcgcgt tcaggtcgcg cgggaacggc agcgtgacgt cgagcacgtc
     3901 cttgatgcgc ccgggccgcg cggtcatcac cacgatgcgg tcggagagcg cgatcgcctc
     3961 ctgcaggctg tgggtgacga agaccaccgt cttgcgggtc cgacggcgga tccccaccag
     4021 ccactccccc atgagcaggc gcgtctgctc gtcgagcgcc ccgaagggct cgtccatcag
     4081 gaggatcggg gtgtccagcg cgagtgcgcg cgcgatcgcc acccgctggc gcatgccacc
     4141 cgacagctcg cgggggtact tccgctcgaa gccggcgagg cccatgggct cgaggagccc
     4201 gcgggcgatc tcccggcgct tcgccgcggg caccccgcgc agctcgaggc cgaaggtcac
     4261 gttgtcgagc gcggtgcgcc acgggaagag gccgccgtcc tggaacacga gcgcgacctt
     4321 gtccgggctc ggcccggtgc gtaccgcgcc gtccagaagc acgcgcccgc tatcgggcgc
     4381 cacgagcccg ccgatcatgc cgagcacggt ggacttgccg cagccgctcg gccccagcag
     4441 cgagacgaac tcctcgcgcc gcaccgtgag gctcaccgag tcgaccgcct ggaccgggcc
     4501 gctggtggtc tcgtagcgct tgccgatcgc gtcgagcacg atcggcgcga gcgcgacggt
     4561 cgcgaccccg tctggcatgc gcgcattatg ctacagtgcg acggaaaagg aggggccatc
     4621 catggcagac gccgtgcatc tgggcagtgg gaaggtccgc gagctctacg ccctcgacga
     4681 cgagcggctc ctgctcgtgg cctccgaccg catctccacc ttcgatgtcg tgctgccgac
     4741 cgccatcccg gacaagggcc gggtgctgac cggcctggcc gccttctggt tcgcgcgcac
     4801 ccgctcgatc tgcccgaacc acctgttggc cctccggccc gacggccgct ccaccgagtg
     4861 ccgccgcctc gagatgctgc cgatcgagtg cgtggtgcgc ggctatctct ccggctcggg
     4921 ctggaaggac tatggcaaga ccggtgaggt ctgcggccac cggctgcccg ccggcctgcg
     4981 cgagtccgac cggctccccg agccgatctt caccccggcc accaaggccc agtccggcca
     5041 cgacgagaac atcacgcgcg agcagggcgc ggcgatcgtc ggggccgagc gcttcgcgga
     5101 ggccgagcgg ctctcgatcg ccctctaccg attcgcggcc gcctacgcgc tcgagcgcgg
     5161 catcatcatc gccgacacca agttcgagtt cggc
//
LOCUS       DATMCV010000455         2344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_960377, whole genome shotgun sequence.
ACCESSION   DATMCV010000455 DATMCV010000000
VERSION     DATMCV010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960377"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2329
                     /locus_tag="VNU03_10475"
     CDS             <1..2329
                     /locus_tag="VNU03_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74934.1"
                     /translation="SWLRGLGVQNADRDTRLLAATIGEAGTEGVDKDLLFSAWRSALE
                     AAARRSPVVIVFEDLHWSSDSLLDLAEFVMQPRGEAPVLMVVLARPELLDRRPTWGGG
                     RLNHLAMALEPLGDTAIADLVRHLLDTEAPDVIKLVSERSEGNPFYAGELVRSYLEQG
                     SLERLPDTVQATVLARLDLLPPLERRVLQLGSVFGRAFRASGVGALESGLAAQAVALC
                     ENLADRDLIRAAEADRFAFRHILIQEVAYSTLPRSERARLHGAAARWVESTSAGREGA
                     VAEILAFHHREAAVLSAALEPGSESTAQAKESAGRWLIKAADVAAAAGATHEAVRHIR
                     GALDFVDPSIRASLHERIGDMTGGDSGVEEYRLALELYAAQGAPVDDQLRALAGMLMV
                     TTRWSGSVGDRPLTEWMDELRARGQGLFARAHDPRSVGRFLAADAFYPFWLQGLRNPT
                     SEELAQAESDANRALQIATELDDPDLASLALDAIAGLAQSTNDWAGARATSLRRTEFE
                     ARLGIYERLDAHSMIAWMSYLMGDLAAADRDSEEMAARLLPGQAPYPALHLFAWRAVT
                     LYALGRWDEAVAVFWRGIESWHDAGRLAAGYGLRGFAVGLDIGRARGDARVTGAAADA
                     MESVLSRFPARHPIRVWDAYIRGESGFTFDDPYLSGRYAFEATERRLCLACDGREKLP
                     SEILEAGLVVALSAHVPLLEAQLRRARALAFREPAELTAALAIWDRIGAVPHQGRGRA
                     ERGLLTGDQAETDAGLAILKKVGDANYVDRFAAQV"
BASE COUNT          346 a          780 c          831 g          387 t
ORIGIN      
        1 gtcgtggctg cgcggcctcg gcgtccagaa cgccgaccgt gacaccaggc tgctcgccgc
       61 caccatcggc gaggccggca ccgaaggcgt cgacaaagac ctgctcttct ccgcctggcg
      121 ctcggcgctg gaggcggccg ctcgccggag ccccgttgtc atcgtgtttg aagacctgca
      181 ctggtccagc gacagcctgc tggacctcgc cgagttcgtg atgcagcccc gcggcgaggc
      241 gccggtgctg atggtcgtgc tcgcacgtcc ggagctgctt gaccgccgcc cgacatgggg
      301 tggaggaaga ctcaaccacc tggccatggc cctggagccc ctcggagaca ccgcaatcgc
      361 cgacctggtg cgtcacctgc tcgacaccga ggctcccgat gtgatcaagc tggtatccga
      421 gcgctcggag ggcaatcctt tctatgccgg agagctggtc cgttcctatc tcgagcaggg
      481 atcgctcgag cgcctgccgg acaccgtgca ggccaccgtg ctcgctcgcc ttgacctgct
      541 gcccccattg gagcggcgcg tcctgcaact tggcagtgtc ttcggacgtg cgttccgtgc
      601 ctccggcgtc ggggcgctcg agagcgggct ggcggcgcag gctgtagcgc tctgcgagaa
      661 cctcgccgat cgcgacctga tccgggccgc cgaagccgac cgttttgcct tccgacacat
      721 cctcatccag gaggtcgcgt acagcaccct gccgcgcagc gagcgcgcgc ggcttcatgg
      781 tgcggccgcg cgctgggtgg agtcgacgag cgcggggcgc gagggggccg tggcggagat
      841 cctggccttc caccaccgcg aggccgcagt gctcagcgcc gcgctcgaac ctggctccga
      901 aagcacggcc caagcaaaag aatcggccgg tcgctggttg atcaaggcgg ccgacgtcgc
      961 agccgccgcg ggcgcgacac acgaggctgt gcgtcatatt cgcggtgcgc tggacttcgt
     1021 cgacccttca atccgggcga gcctccacga gcgcatcggg gacatgacgg gcggtgacag
     1081 cggagtcgag gaataccggc ttgccctgga gttgtacgcg gcgcagggcg cgccggtcga
     1141 tgatcagctc agggcgctgg ccggcatgct catggtcacg acccggtggt ccggatcggt
     1201 aggggaccgg ccgttaacgg aatggatgga cgagctccgc gcacgcggcc agggcctgtt
     1261 tgcccgggcc cacgatccgc gctccgtcgg acgcttcctg gcggcggatg cgttttatcc
     1321 attctggctc cagggcctcc ggaacccgac ttcggaggag ctcgcgcagg cggagtcgga
     1381 cgcgaatcgg gcattgcaga tcgccaccga gctcgacgat cctgacctcg cgagcctggc
     1441 tcttgatgcg atcgcgggcc tcgcccagtc gactaatgat tgggccggcg cccgggccac
     1501 ctcgctgcgg cggacggagt tcgaagccag gctgggcatc tacgaacggc tggacgccca
     1561 ctcgatgatc gcctggatgt cgtacctgat gggcgacctg gcggccgccg atcgcgactc
     1621 ggaagagatg gcggcacgtc ttttgccggg ccaggcgcct tacccggccc tgcatctctt
     1681 cgcctggcgg gcggtcacgc tgtacgcgct cggccgatgg gacgaggcgg tcgccgtctt
     1741 ctggcgaggc atcgaatcgt ggcatgacgc cggccggctg gccgcgggtt atgggctgcg
     1801 aggatttgcg gtcggcctcg acatcgggcg ggcgcgaggg gacgcgcggg ttaccggcgc
     1861 ggccgccgat gcaatggaat cggtgctgtc tcgcttcccg gccagacacc ccatccgggt
     1921 ttgggatgcg tacattcgag gcgaaagcgg gtttacgttt gacgacccgt acctatcggg
     1981 ccgctatgca ttcgaagcca ccgagcggcg cctttgcctg gcttgtgacg ggcgcgagaa
     2041 gctcccgagc gagattctgg aagcaggttt ggtggtggcg ctgagcgctc acgtaccact
     2101 cctcgaggct cagctcaggc gcgccagagc gctcgccttt cgcgagcccg cggagctcac
     2161 agccgcgctg gcgatctggg atcggatcgg cgcggtgccg caccagggcc gcgggagggc
     2221 ggagcgcggt ctgctcaccg gcgaccaggc cgagaccgac gccggtctcg ccatcctgaa
     2281 gaaggtgggc gatgcgaact acgtggacag gttcgcggct caggtctgac cggtcgcatg
     2341 tcga
//
LOCUS       DATMCV010000456         5277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_556336, whole genome shotgun sequence.
ACCESSION   DATMCV010000456 DATMCV010000000
VERSION     DATMCV010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556336"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /gene="pgk"
                     /locus_tag="VNU03_10480"
     CDS             <1..351
                     /gene="pgk"
                     /locus_tag="VNU03_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWP74935.1"
                     /translation="RTVGIREIPDDLMGLDIGAATVTQFTTALRGARTIVWNGPLGVF
                     EKPAFAAGTLGVAHAVAGSGAFSVIGGGDTIAAVQQAGVMDKIGYVSTAGGAFLEFLE
                     GRVLPGVAALDDAA"
     gene            351..1130
                     /gene="tpiA"
                     /locus_tag="VNU03_10485"
     CDS             351..1130
                     /gene="tpiA"
                     /locus_tag="VNU03_10485"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWP74936.1"
                     /translation="MRTPLVVGNWKMYGTQDAARELALAVRDGLKRPRGVEVVVCPPF
                     TALPAVAEVLKGAAVALGAQNCHWEAQGAFTGEISPAMLAELGCRWVLLGHSERRHVF
                     HETDEEINRKVVAVLRHGMRPVLCVGETDEERRQGLTFTVVEGQLRAGWSGLSPEDLA
                     RCVLAYEPVWAIGTGVNATPAQAGEVHSYLRGLVSEVASKELAQSMRILYGGSVKPDN
                     AQALTQEPDIDGVLVGGASLQAPGFITIAKKSAARSGASKE"
     gene            1137..1463
                     /gene="secG"
                     /locus_tag="VNU03_10490"
     CDS             1137..1463
                     /gene="secG"
                     /locus_tag="VNU03_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWP74937.1"
                     /translation="MFILVVIVHIIVCLVIVGLVLLQAGKGADIGSAFGGSGSQAVFG
                     SMGTPTVLGKATSVVAIMFMVTSFALSMMAHKRTLSIMPATGPAAPAPATPAAPPVPA
                     TPAPQK"
     gene            1476..3137
                     /locus_tag="VNU03_10495"
     CDS             1476..3137
                     /locus_tag="VNU03_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HWP74938.1"
                     /translation="MPSRRARIRALLAWTAGLGLAAACNGPIESRPDEVAATGPPAYG
                     DTFIQASIGDIGGLIPSLTSDQSSHEVGGLIYDGLVKQDKDLNLVPAMAESWTYSPNC
                     LELSFKLRRDVKWHDDYPFTADDVVFTYETMINPKTPAPFKEGFLLVKEAKALDKYTV
                     RIRYDKPYARAVETWGTTMLPKHLLQSFADAGTLRESPQNSLPVGTGPYRFQEWKPGE
                     KVVLVANPDYYEGRPFLSRIVYRVIPSQATIFLELKANGVDYVNTLTGIQYARQTEYP
                     AFRKAYHKFRYPGDNYTFLGFNLKDPRFADKRVRQAFAHAIDKQELIDGVRLGLAHEA
                     NGPMRPGTWAYTENVKHYEYSPEKAKALLAEAGWKDRDGDGVLKDGDGKPFTLTIRTN
                     QGNDERKKIAEIVQQRLKEIGIQADIQLIEWAAFIKEFIKPRRFELVVLGLGTGADPD
                     QYVVWHSSQRGPDQMNRTGYSNPEVDQLLEVGRASCIQKDRVRVYHRIQEILAEDLPM
                     IFLFNRDSLPVVTSRIRGVSPSPSGILYNFNEWYVPKTQQRYTSG"
     gene            3138..4130
                     /locus_tag="VNU03_10500"
     CDS             3138..4130
                     /locus_tag="VNU03_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74939.1"
                     /translation="MALFALRRLLLSIPLLIGITFVSYAVISLAPGGPLDFLTPDDPN
                     ASPEVKERLIKEFGLDRPIHVQYWQWLKRAVRLDFGRSFLPDGRPVLTKIGERLPVTL
                     FLNVIELAIIVGLAVPIGVLSATRQYSLYDKITTVFVFVGFATPDFWLALLLMILFGV
                     QLGWLPISGLRSLNWEYLSFWQQQWDFFSHLVLPIVVATFGGLAGFSRYMRQSMLEVV
                     RQDYVQTARAKGLSERVVISKHALRNALLPVITILALALPGLIGGSVIIESIFAIPGM
                     GQLMVQSVFSRDYPVVMGNLVIVASLTLVANLVADITYGLVDPRIRFGGRRRRR"
     gene            4130..5023
                     /locus_tag="VNU03_10505"
     CDS             4130..5023
                     /locus_tag="VNU03_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74940.1"
                     /translation="MAPGQTRRGEIRQFWRTFSRNQLAMCGGAVVTVLVFLAVLAPAL
                     APWDPNRPDTRKILIAPSSSHWFGTDPLGRDVLSRVLYGSRISLSVGFVSMGIAAFIG
                     VLLGAAAGYYGGMVDNVVMRLVDLMLVFPRFFLLLAVLAFLKPSIWTIMTVIGVTGWM
                     GVARLVRAEFLTLREREFVIWSESVGAGALRIIFRHILPNAIAPILVAMTLGIPAAIL
                     TEAGLSFLGLGVQPPYATWGNILNDGRDYIEIAWWLTLYPGLAILITVLSYNLMGEGI
                     RDALDPRLRQSRVGRILRLGR"
     gene            5073..5157
                     /locus_tag="VNU03_10510"
     tRNA            5073..5157
                     /locus_tag="VNU03_10510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5107..5109,aa:Leu,seq:gag)
BASE COUNT          782 a         1867 c         1756 g          872 t
ORIGIN      
        1 cgcacggtgg gtatccgcga gatccccgac gacctcatgg ggctcgacat cggcgcggcc
       61 accgtgactc agttcaccac cgcgctccgg ggggctcgca ccatcgtgtg gaacgggccc
      121 ctgggcgtgt tcgagaagcc cgccttcgcg gccggcaccc tcggggtggc gcacgcagtg
      181 gcgggctcgg gcgccttctc ggtgatcggg ggcggcgaca ccatcgcggc ggtgcagcag
      241 gccggggtca tggacaagat cggctacgtg tccaccgcgg gcggcgcctt cctcgagttc
      301 ctcgaaggac gcgtgctgcc cggcgtggcg gccctggacg acgcggcctg atgcgcaccc
      361 cgctcgtggt cggcaactgg aagatgtacg gcacgcagga cgcggcccgc gagctggccc
      421 tcgcggtgcg cgacgggctc aagcgcccgc gcggggtcga ggtcgtggtg tgcccgccct
      481 tcaccgcgct gcccgcggtg gccgaggtgc tgaagggcgc cgcggtcgcg ctcggcgcgc
      541 agaactgcca ctgggaggcc cagggggcgt tcaccggcga gatctcgccc gcgatgctcg
      601 ccgagctggg ctgccgctgg gtcctgctcg gccactcgga gcgtcggcac gtcttccacg
      661 agaccgacga ggagatcaac cgcaaggtcg tcgcggtcct ccgccacggc atgcggccgg
      721 tgctctgcgt gggcgagacg gacgaggagc gacgccaggg gctcaccttc acggtggtgg
      781 aagggcagct ccgcgcgggc tggtccggtc tctcccccga agatctcgcg cgctgcgtcc
      841 tcgcctacga gccggtgtgg gccatcggca ccggggtcaa cgcgaccccc gcccaggccg
      901 gcgaggtgca cagctacctc cgcggcctgg tctccgaggt cgcgtccaag gagctggccc
      961 agtcgatgcg gatcctctac ggcggcagcg tcaagcccga caacgcccag gcgctcaccc
     1021 aggagccgga catcgacggc gtcctggtgg gaggagccag tctccaggcg cccggcttca
     1081 tcaccatcgc caagaagtcg gccgcccgca gcggcgcttc gaaggagtag cgctccatgt
     1141 tcatcctggt cgtgatcgtc cacatcatcg tgtgcctggt catcgtcggc ctggtgctgc
     1201 tgcaggccgg caagggcgcc gacatcggct ccgccttcgg cggcagcggc agccaggcgg
     1261 tgttcgggtc catgggcacg cccaccgttc tcggcaaggc cacctcggtg gtggccatca
     1321 tgttcatggt cacctcgttc gccctctcga tgatggcgca caagcggacg ctgagcatca
     1381 tgcccgcgac gggtcccgcc gcgcccgcgc cggccacgcc cgcggcgccg cccgtcccgg
     1441 ccacgcccgc gccccagaag tagtcgtccc cgggcatgcc gagtcgtcgc gcgcgcattc
     1501 gcgccctgct ggcctggacc gccggtctcg gtctcgcggc ggcctgcaac gggccgatcg
     1561 agagccgccc cgacgaggtc gcggcgaccg gcccgcccgc ctacggcgac accttcatcc
     1621 aggcctccat cggcgacatc gggggcctga tcccgagcct cacctccgat cagtcttcgc
     1681 acgaggtggg cgggctgatc tacgacgggc tcgtgaagca ggacaaggac ctgaacctgg
     1741 tccccgcgat ggccgagtcc tggacgtaca gcccgaactg tctcgagctg agcttcaagc
     1801 tccgtcggga cgtgaagtgg cacgacgact acccgttcac ggccgacgac gtggtcttca
     1861 cctacgagac catgatcaac ccgaagaccc ccgcgccgtt caaggagggc ttcctcctgg
     1921 tcaaggaggc gaaggccctc gacaagtaca ccgtccggat ccgctacgac aagccgtacg
     1981 cgcgggcggt ggagacgtgg ggcacgacga tgctgcccaa gcacctgctc cagtcgttcg
     2041 cagacgcggg caccctgcgc gagtcgcccc agaacagcct gccggtcggg acgggcccct
     2101 accgcttcca ggagtggaag ccgggggaga aggtggtgct ggtcgccaac cccgactact
     2161 acgagggccg cccgttcctg agccgcatcg tgtaccgggt catcccgagc caggccacga
     2221 tcttcctgga gctgaaggcc aacggggtcg actacgtcaa caccctcacc gggatccagt
     2281 acgcgcgcca gaccgagtac ccggcgttcc gcaaggcgta tcacaagttc cgctatccgg
     2341 gagacaacta caccttcctc ggcttcaacc tcaaggatcc gcgcttcgcg gacaagcgcg
     2401 tgcgccaggc cttcgcccac gccatcgaca agcaggagct gatcgacggg gtgaggctcg
     2461 ggctggccca cgaggccaac gggccgatgc gcccgggtac gtgggcctac accgagaacg
     2521 tgaagcacta cgagtacagc cccgagaagg ccaaggccct gctggccgag gcgggctgga
     2581 aggaccggga cggcgacggg gtgctgaagg acggcgacgg caagccgttc acgctgacca
     2641 tccggaccaa tcagggcaac gacgagcgga agaagatcgc cgagatcgtc cagcagcgcc
     2701 tgaaggagat cggcatccag gccgacatcc agctcatcga gtgggccgcg ttcatcaagg
     2761 agttcatcaa gccgcggcgg ttcgagctgg tggtcctggg tctgggcacc ggcgccgatc
     2821 ccgatcagta cgtcgtctgg cactcgtcgc agcgcggccc ggatcagatg aaccggaccg
     2881 gctactccaa cccggaggtc gatcagctcc tggaggtggg gcgcgcgtcg tgcatccaga
     2941 aggaccgagt gcgcgtttac catcggatcc aggagatcct cgccgaggac ctgccgatga
     3001 tcttcctctt caaccgggac tcgctcccgg tggtgacctc gcgcatccgg ggggtgagcc
     3061 cgtccccctc cggcatcctg tacaacttca acgagtggta cgtgccgaag acacagcagc
     3121 gctatacgtc cggctagatg gcgctcttcg ccctccggag gctcctcctc tcgatcccgc
     3181 tcctgatcgg catcacgttc gtctcctacg cggtcatcag cctcgccccc ggcggcccgc
     3241 tcgacttcct gaccccggat gatcccaacg cgtcgccgga ggtgaaggag cggctgatca
     3301 aggagttcgg gctcgaccgg cccatccacg tgcagtactg gcagtggctc aagcgcgcgg
     3361 tccggctcga cttcgggcgc tccttcctgc ccgacgggcg gccggtcctc accaagatcg
     3421 gcgagcgcct gccggtgacc ctgttcctca acgtgatcga gctggccatc atcgtggggc
     3481 tcgcggtgcc catcggcgtg ctctcggcca cccgccagta ctcgctctac gacaagatca
     3541 cgaccgtctt cgtcttcgtg ggcttcgcca ccccggattt ctggctcgcg ctgctgctga
     3601 tgatcctctt cggggtccag ctcggctggc tgcccatctc cgggctgcgc tcgctcaact
     3661 gggaatacct ctcgttctgg cagcagcagt gggacttctt ctcccacctg gtcctgccca
     3721 tcgtggtcgc caccttcggg ggactcgccg gcttctcgcg ctacatgcgc cagagcatgc
     3781 tggaggtggt gcgccaggac tacgtgcaga ccgcgcgggc caagggcctc tcggagcggg
     3841 tggtgatcag caagcacgcc ttgcgcaacg cgctgctccc ggtgatcacg atcctggccc
     3901 tggccctgcc cggcctcatc gggggcagcg tgatcatcga gtccatcttc gcgatccccg
     3961 ggatgggcca gctcatggtg cagtccgtct tctcgcgcga ctacccggtg gtgatgggca
     4021 acctggtcat cgtggcgagc ctcaccctgg tggccaacct cgtcgccgac atcacctacg
     4081 ggctcgtgga tccccgcatc cgattcggtg gtcggcggag gaggcgctag tggcgcccgg
     4141 acagacgcgg cgcggcgaga tccgtcagtt ctggcgcacg ttctcccgca atcagctcgc
     4201 gatgtgcggg ggggcggtgg tcacggtgct ggtgttcctg gccgtgcttg cacccgcgct
     4261 ggctccctgg gacccgaacc ggccggacac ccggaagatc ctgatcgccc cgtcgagcag
     4321 ccactggttc ggcaccgatc ccctcgggcg ggacgtgctc tcccgggtgc tctatggctc
     4381 gcggatttcc ctctcggtcg gcttcgtctc gatgggcatc gcggcgttca tcggggtgct
     4441 cctgggcgcg gcggccggct actacggcgg gatggtcgac aacgtggtga tgcggctggt
     4501 ggacctgatg ctggtctttc cccgcttctt cctcctgctc gccgtcctcg cgttcctcaa
     4561 gccgtccatc tggaccatca tgaccgtgat cggcgtcacc ggctggatgg gggtggcccg
     4621 gctcgtgcgc gcggagttcc tgaccctgcg cgagcgcgag ttcgtcatct ggtcggagtc
     4681 ggtgggagcg ggggcgctgc gcatcatctt ccgccacatc ctgcccaacg ccatcgcgcc
     4741 gatcctggtc gccatgacgc tcggcatccc ggccgccatt ctcaccgagg cggggctctc
     4801 cttcctggga ctgggcgtcc agccgcccta cgccacgtgg gggaacatcc tcaacgacgg
     4861 gcgcgactac atcgagatcg cctggtggct gaccctctat ccgggcctgg ccatcctgat
     4921 cacggtactt tcctacaatc tgatgggcga ggggatccgc gacgcgctcg acccgcgcct
     4981 gcgccagtcc cgcgtcggcc gcatcctgcg tctcggccgc tagccgaatt gacaaggctc
     5041 gcgggggcgc ggcatactgt gccctcgtcg gcgccgaagt ggcggaattg gcagacgcgc
     5101 acgtttgagg ggcgtgtggg gcaacccgtg ggggttcgag tccccccttc ggcaccactg
     5161 atcggtaagt catcgaccca ccgagtctaa ccggaagcgc gcgtcctcac cgggagtatt
     5221 tcgggagtac cgcacggccc accggctcgg tctccgcctc ctgccgctcg gtcatcc
//
LOCUS       DATMCV010000457         7668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_358414, whole genome shotgun sequence.
ACCESSION   DATMCV010000457 DATMCV010000000
VERSION     DATMCV010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358414"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2196)
                     /locus_tag="VNU03_10515"
     CDS             complement(<1..2196)
                     /locus_tag="VNU03_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP74941.1"
                     /translation="MRMDKLTVKAQEAIQAAQSLADQGNHQAIEPEHLLLALLQQQEG
                     VVGPLLGKLGARPEALARQIQAEVDKLPAVRGGAGRQYASSRFEGVINRAREEAERLK
                     DEYVSTEHLLIAIAQEKGGASARLLAAAGVTPEAIYRSLVEVRGSQRVTDPNPEDKYQ
                     ALQRYSKDLTELARKGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVAGDVPEGLKNKRLVAVDIGAMVAGAKYRGEFEDRLKAVLREITESNGEIIC
                     FIDELHTLVGAGAAEGAVDAANMLKPALARGELRCVGATTLDEYRKHIEKDPALERRF
                     QPVLVREPSVEDTIAILRGLKEKYEVHHKVKIKDAALVAAAVLSHRYIADRFLPDKAI
                     DLIDEASSRLRIEIDSMPAELDEIRRRIMQLEIERVSVARESDEVSRERLARLDSELA
                     ELREKSSQLQSQWQAEKAAIMTIGKIKEEGEAARAAMADAERRGDLQKAAELRYGTLM
                     TIDKRLEAESVRLHELQKGGRMLKEEVDEEDVAETVAKWTGIPVTRLLEAEVQKLVKM
                     EERLAERVVGQREAIVAVSNAVRRARSGLSDPNRPIGSFLFLGPTGVGKTELARALAE
                     FLFDDERAMIRIDMSEYMEKHTVARLIGAPPGYVGYDEGGQLTEAVRRRPYSVLLFDE
                     IEKAHPDVFNVLLQLLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHFFREE"
     gene            complement(2198..3268)
                     /locus_tag="VNU03_10520"
     CDS             complement(2198..3268)
                     /locus_tag="VNU03_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWP74942.1"
                     /translation="MEFKDYYKTLGVERAADDKAIKTAYRKLARKYHPDVNKGNAERF
                     KEISEAYTVLSDPEKRKRYDTLGPDWERYAQAGAGAASGGRSPFEGRPFGGRDVRFTQ
                     EGDAGGFSDFFRTIFGGDLGGFRRGEPGGGRTDFEFSGFGDPGADLGGGMGRGNDVEA
                     AIELTLEEAFNGARKTIALELDEPCPQCGGSGNVKGRPCPRCHGSGWTKATRNLDVKI
                     PAGVDTGSRVRVAAEGPSGGRGDRSDRGDLYLRVTVAPHARFERKGADLHLELPLPVT
                     VAALGGEVSVPTLKGQVSMKIPAETSSGRTFRLRGYGMPHLKGGGAGDEFVKVQIVIP
                     SGLSPREKELFEELKKLRAETN"
     gene            3412..5490
                     /locus_tag="VNU03_10525"
     CDS             3412..5490
                     /locus_tag="VNU03_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP74943.1"
                     /translation="MAKTCTLAIDVGGTFTDITFTDAATGALWVAKTPSTPGDLSTGF
                     ITAVRKVLALAGRAPGDVLRVFHGTTTATNAILEGKTPPTALVTTAGFKYVLEIGRHD
                     IPRHGNLYGWSKPTRPITPERVFEVPERLDVDGSVLTPLDEPAARLLARQLAQLGLPA
                     VAVVFLHAYANPAHEQRMQAILAEEYPGVLVSLSSEVLPQFREFERTMATSLNAAVMP
                     PVSRYVSVLRRALDGEGVQAPLLIMKSDGGVTSAATCVRQPVQTVLSGPAAGVVGAVS
                     VGRAAGFGDIISIDVGGTSADICLVRDGRPEVTKDGMIGPFPLKLPMVDIHTIGAGGG
                     SIAAVTSAGRLTVGPRSAGAEPGPVCYGRGGSEPTVTDAHLVLGRIPAALLGGELPLD
                     VAAARAAIAARIGRPLGLDAEEAAAGIVEIIDNAMARAIRTVSVGRGHDPRRFALVAF
                     GGAGPLHACRLAELLDIPVVVVPPRPGVLSTWGLLDTDIRATFVKTVGVAERRLAEAG
                     SQVARLEAAWTDLERQAQGWLAGEQVPPEGQRFERAADLRYEHQSFELTCPIGEGPIT
                     GARLQALTATFHAEHRRLYSYDLPSAPVELVNLRLTAVGALPRRETRPPTAAAGGLAQ
                     ARTGSRPVYFRGVGFVETPSYARDRLSPGLTLEGPAIIEQSDATPLIAPGFRARVDAA
                     GHILLERMSG"
     gene            complement(5732..5983)
                     /locus_tag="VNU03_10530"
     CDS             complement(5732..5983)
                     /locus_tag="VNU03_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74944.1"
                     /translation="MDSASQRIAEKIAARILPAVADPVSVWEGFGIGLHCDGCDLPVL
                     PSEPQQELALPDGRTLRFHASCAAAWRRLRDVLPLRQMK"
     gene            complement(5991..6452)
                     /locus_tag="VNU03_10535"
     CDS             complement(5991..6452)
                     /locus_tag="VNU03_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74945.1"
                     /translation="MRTLLALSLLAAGCSASPAWLSHPWRSEKVARPIDEPREPASIG
                     AAARSRGELSTDPAEALYRLGVRQADPGSGIRDYRAARATFTELLIQYPHSRRDAEAR
                     AWQATLTDLLVREDDFRRALLRLRRSEEDGKRTKTNLEWLRQNDLDQERRK"
     gene            complement(6464..6847)
                     /locus_tag="VNU03_10540"
     CDS             complement(6464..6847)
                     /locus_tag="VNU03_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP74946.1"
                     /translation="MSRAISPSTTARIVVVDDDDHVREVTEMVLAGAGHSVRATPNGL
                     EALRWLEEEPWDLLILDMRMPEIDGPALYREVLARWPRAASHVLFVSGMTERPPDRPL
                     DVPVLVKPFSLEELLAAVARVLATI"
     gene            complement(7041..7412)
                     /locus_tag="VNU03_10545"
     CDS             complement(7041..7412)
                     /locus_tag="VNU03_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74947.1"
                     /translation="MRDEGDLLGYAAVAGIIGAIALYRFLIRREQDRNWLRAKATGAI
                     QELIARGTHLHRFGAGGPDDPRSAGEAWRETVARTLRGAPFKAGADRFVLENAGEHFA
                     GPLQRLCEILAAPDPWINFET"
BASE COUNT         1000 a         2764 c         2708 g         1196 t
ORIGIN      
        1 ctcctcgcgg aagaagtggc tcccgaggtt ggaggtcatg atgaccaccg tgttacggaa
       61 gtccaccgtg cggccctggc cgtcggtgag acggccgtcg tcgaggagct gcaggagcac
      121 gttgaagacg tcggggtggg ccttctcgat ctcgtcgaag agcaggaccg agtacggccg
      181 ccggcgcacc gcctcggtga gctggccgcc ctcgtcgtag cccacgtagc cggggggcgc
      241 cccgatgagg cgggccaccg tgtgcttctc catgtactcg gacatgtcga tgcggatcat
      301 ggcccgctcg tcgtcgaaga ggaactcggc gagcgcgcgc gccagctcgg tcttgcccac
      361 cccggtcggg cccaggaaca ggaacgagcc gatggggcgg ttggggtcgg agagaccgga
      421 gcgggcccgg cgcaccgcgt tggagaccgc cacgatcgcc tcgcgctggc cgaccacccg
      481 ctcggccagc cgctcctcca tcttcaccag cttctgcacc tcggcctcga ggagccgggt
      541 gaccgggatg ccggtccact tggccaccgt ctcggccacg tcctcctcgt cgacctcctc
      601 cttgagcatc cggccgccct tctgcagctc gtgcaggcgc acgctctcgg cctcgaggcg
      661 tttgtcgatc gtcatcaggg tgccgtagcg cagctcggcc gccttctgca gatccccgcg
      721 ccgctccgcg tcggccatcg cggcgcgcgc ggcctcgccc tcctccttga tcttgccgat
      781 ggtcatgatc gcggccttct cggcctgcca ctgactctgg agctgggagg acttctcgcg
      841 cagctcggcc agctcggagt cgagccgggc cagccgctcg cgcgagacct cgtcggactc
      901 gcgggccacc gagacccgct cgatctcgag ctgcatgatg cgccgccgga tctcgtccag
      961 ctcggcgggc atcgaatcga tctcgatgcg cagccgggac gaggcctcgt cgatcaggtc
     1021 gatggccttg tcgggcagga agcggtccgc gatgtagcgg tgcgagagca ccgccgcggc
     1081 caccagcgcc gcgtccttga tcttcacctt gtggtggacc tcgtacttct ccttgaggcc
     1141 gcgcaggatc gcgatggtgt cctccaccga gggctcgcgc accagcaccg gctggaagcg
     1201 acgctcgagc gcggggtcct tctcgatgtg cttgcggtac tcgtccaggg tggtggcgcc
     1261 cacgcagcgc agctcgccgc gggccagggc cggcttgagc atgttggccg cgtcgaccgc
     1321 cccctccgcc gcccccgcgc ccaccagggt gtgcagctcg tcgatgaagc agatgatctc
     1381 gccgttcgac tcggtgatct cgcgcagcac cgccttcagg cggtcctcga actcaccccg
     1441 gtacttggcg cccgcgacca tggccccgat gtcgaccgcg accagccgct tgttcttgag
     1501 cccctccggc acgtcgccgg cgacgattcg ctgcgccagt ccttcgacga tggcggtctt
     1561 gcccactccg ggctcgccga tcagcaccgg gttgttcttg gtgcggcggg agagcacctg
     1621 gatgacgcgg cggatctcct cgtcccggcc gatgaccgga tcgagcttgc ccttgcgggc
     1681 gagctcggtc aggtccttgg agtagcgctg gagggcctgg tacttgtcct ccgggttggg
     1741 atcggtgacg cgctgggagc cgcgcacctc gaccagcgag cggtagatcg cctccggggt
     1801 cacccccgcc gcggccagga ggcgcgcgct ggcgccgccc ttctcctgcg cgatggcgat
     1861 gagcaggtgc tcggtggaga cgtactcgtc cttcaggcgc tcggcctcct cgcgggcgcg
     1921 gttgatcacg ccctcgaagc gcgacgaggc gtactgtcgc ccggcgcctc cgcggacggc
     1981 gggcagcttg tccacctccg cctggatctg gcgcgcgagc gcctccgggc gcgcccccag
     2041 cttgccgagg agcgggccga cgaccccctc ctgctgctgc aggagcgcga gcagcaggtg
     2101 ctcgggctcg atggcctggt gattgccctg gtcggccagg gactgcgcgg cctggatcgc
     2161 ctcctgggcc ttgacggtca gcttgtccat cctcatctca gttggtctcc gctctgagct
     2221 tcttcagctc ctcgaacagc tctttctctc tcgggctgag cccgctcggg atcacgatct
     2281 gcaccttcac gaattcgtcg ccggccccgc cccccttgag atgcggcatc ccgtagccgc
     2341 ggagccggaa ggtcctgccg ctcgaggttt cggccgggat cttcatcgag acctggccct
     2401 ttagggtcgg caccgagact tcgcccccga gcgcggccac cgtcaccggc agcggcagct
     2461 ccaggtgcag gtcggcgccc ttgcgctcga agcgggcgtg gggggccacc gtcacgcgca
     2521 ggtagagatc cccacggtcg ctccgatcgc cgcgcccgcc gctcggccct tccgccgcca
     2581 cccgcacccg cgagccggtg tccacgccgg ccgggatctt cacgtccagg ttccgggtgg
     2641 ccttggtcca gccgctcccg tggcagcgcg ggcaggggcg gcccttcacg ttgccggacc
     2701 cgccgcactg ggggcacggc tcgtcgagct cgagcgcgat cgtcttgcgc gcgccgttga
     2761 aggcctcctc gagcgtgagc tcgatggccg cctccacgtc gttgccgcgc cccatgccgc
     2821 cgcccaggtc ggccccgggg tcaccaaaac ccgagaactc gaagtcggtc cgcccgccac
     2881 ccggctcgcc ccgacggaag ccgcccaggt caccgccgaa gatggtgcgg aagaagtccg
     2941 agaacccgcc cgcgtcgccc tcctgggtga agcggacatc tcgacccccg aaggggcggc
     3001 cctcgaacgg ggatcgcccg cccgaagcgg cgccggcgcc ggcctgggca tagcgctccc
     3061 agtcggggcc gagggtgtcg tagcgcttgc gcttctccgg atcggagagg accgtgtagg
     3121 cctcgctgat ctctttaaag cgctccgcgt tccccttgtt gacgtcgggg tggtacttgc
     3181 gggcgagctt gcggtaggcg gtcttgatcg ccttgtcgtc cgccgcgcgc tcgaccccga
     3241 gggtcttgta gtagtctttg aattccatgt ggttagacga caaagcggcg ccgggctcac
     3301 gagtccgcgc cgcttgagtc ggctagggga agtgtagcag acggttcgag ggttggctgg
     3361 cggcgccgca tctttcccgg ggcctcgccc gggtctacaa tggccgggcc cgtggccaag
     3421 acctgtacgc tcgccatcga cgtcggcggt accttcaccg acatcacgtt caccgacgcg
     3481 gccaccggag cgctctgggt ggcgaagaca ccctccacgc cgggcgatct ctcgaccgga
     3541 ttcatcaccg cggtccgcaa ggtgctggcc ctggccgggc gcgcccccgg cgacgtgctg
     3601 cgggtcttcc acggcaccac caccgcgacc aacgcgatcc tcgagggcaa gacgcccccg
     3661 accgcgctgg tgacgacggc cggcttcaag tacgtgctcg agatcggccg gcacgacatc
     3721 ccgcgccacg gcaacctcta cggctggtcc aagcccacgc ggcccatcac cccggagcgc
     3781 gtcttcgagg tccccgagcg gctcgacgtc gacggctcgg tgctcacccc gctcgacgag
     3841 ccggcggccc ggctcctcgc ccgccagctc gcgcagctgg ggctgccggc ggtggcggtg
     3901 gtgttcctcc acgcctacgc caatcccgcg cacgagcagc gcatgcaggc gattctggct
     3961 gaggagtatc ccggcgtgct ggtgtcgctc tcctcggagg tgctgcccca gttccgcgag
     4021 ttcgagcgga ccatggccac ctccctcaac gcggcggtga tgccgccggt gagccgctac
     4081 gtgagcgtgc tgcgccgcgc gctcgacggg gagggcgtgc aggccccgct gctcatcatg
     4141 aagtcggacg gtggcgtgac cagcgcggcc acctgcgtgc gccagccggt gcagaccgtg
     4201 ctctccggcc ccgccgcggg tgtggtgggg gcggtgagcg tgggccgcgc cgcgggcttc
     4261 ggcgacatca tctcgatcga tgtgggcggc accagcgccg acatctgcct cgtgcgcgac
     4321 gggcgacccg aggtcaccaa ggacggcatg atcggaccct tcccgctcaa gctgccgatg
     4381 gtggacatcc acaccatcgg ggcgggcggc gggtcgatcg cggcggtgac cagcgccgga
     4441 cgtctcaccg tcggcccgcg cagcgccggg gccgagccgg gcccggtctg ctacgggcgg
     4501 ggaggcagcg agcccaccgt caccgacgcc cacctcgtgc tcgggcgcat cccggccgcc
     4561 ctgctcggcg gcgagctgcc cctcgacgtg gccgcggcgc gggcggccat cgcggcgcgc
     4621 atcggccggc cgctcggcct cgacgcggag gaggctgccg cggggatcgt cgagatcatc
     4681 gacaacgcga tggcgcgggc catccgcacc gtgtcggtgg gacgcggcca cgatccgcgc
     4741 cgcttcgcgc tggtggcatt cggaggcgcg gggccgcttc acgcctgccg gctggccgag
     4801 ctgctcgaca tccccgtcgt ggtcgtcccg ccgcgccccg gcgtgctctc gacgtggggc
     4861 ctgctcgata ccgacatccg tgcgacgttc gtgaagacgg tcggcgtcgc ggagcgacgg
     4921 ctcgccgagg cggggagtca ggtagcccgg ctcgaggccg cgtggaccga tctcgagcgt
     4981 caggcccagg gctggctcgc gggcgagcag gtgccgcccg agggacagcg gttcgagcgc
     5041 gcggcggacc tgcgctacga gcaccagagc ttcgagctga cctgtccgat cggggagggg
     5101 ccgatcaccg gagcgcggct gcaggcgctg accgccacgt tccacgccga gcaccggagg
     5161 ctctacagct acgatctgcc ctcggcgccg gtggagctgg tgaacctgcg gctcaccgcg
     5221 gtgggcgcgc tgccgcgccg agagacgcgt cctcccaccg cggccgccgg tgggctggcg
     5281 caggcccgca ccggcagccg gccggtgtac ttccgcggcg tcggctttgt cgagacgccg
     5341 tcctacgcgc gggaccggct ctcgcccggc ctcaccctcg aggggccggc gatcatcgag
     5401 cagagcgacg ccactccgct gatcgcgccg ggcttccgcg cgcgcgtgga cgcggcgggc
     5461 cacatcctcc tggagcgaat gtcgggctag ccgggcacgg aatgcccgga cctcctcgga
     5521 cgtctcatga gtagaggagg tgcgctatgc gctatctgaa gatcgcgttg ttgctcctgg
     5581 cggtcacctt cggaactcaa gcctgtattt tgccggttcc ggtcggcggg gggtggcatc
     5641 accaccacgg ctggcgctga gcggacggga tagcgggagg gtcatggccc gaaggggtcg
     5701 cgccgccgtc gagcggccgc cccttcgggc tctacttcat ctggcgcagc ggcagaacat
     5761 cccgcagccg gcgccaggcc gccgcgcagg aggcgtggaa cctgagggtc cggccgtcgg
     5821 ggagcgcgag ctcctgctgc ggctcgctcg ggaggacggg aaggtcgcag ccgtcacagt
     5881 gcaggccgat cccgaagccc tcccacacgc tgaccggatc ggcgacggcg ggcaggatgc
     5941 gcgcggcgat cttctccgct atccgctggg acgcgctgtc catgcgcggc ctacttgcgg
     6001 cgctcctggt cgaggtcgtt ctgccggagc cactcgagat tcgtcttcgt gcgcttgccg
     6061 tcttcctcgc tgcgccgcag gcggagcagg gcccggcgga agtcgtcctc gcggacgagc
     6121 agatcggtca gcgtcgcctg ccaggcgcgc gcctcggcgt cccgacggct gtgcgggtac
     6181 tggatgagca gctcggtgaa cgtcgcgcgc gccgcgcggt agtcccggat gccgctcccg
     6241 ggatccgcct gccgcactcc gagacggtag agcgcctcgg ccgggtcggt cgagagctcc
     6301 ccgcggctcc gcgcggcggc gccgatcgag gccggctcgc gcggctcgtc gatcggccgc
     6361 gccacttttt ccgaccgcca cggatgcgag agccaggccg gcgacgcgct gcagccggcg
     6421 gcgagcaggg agagcgcgag gagggtacgc atgagcggcg tgttcatatc gtcgccagca
     6481 cgcgggcgac ggcggccagg agctcctcga gcgagaaggg tttgaccagg accggaacgt
     6541 cgagaggccg atcgggaggc cgttcggtca tgccggagac gaacaggacg tgcgaggcgg
     6601 cgcggggcca gcgcgccagg acctcgcgat agagcgccgg tccgtcgatc tcgggcatcc
     6661 gcatgtcgag gatcagcaga tcccacggtt cctcctcgag ccaccggagg gcctccaggc
     6721 cgttcggcgt ggcgcgcacg gaatgcccgg ctcccgcgag gaccatctcg gtcacttccc
     6781 gcacgtggtc atcgtcgtcg acgacgacga ttcgtgcggt ggtcgacggt gaaatcgcgc
     6841 ggctcatcgg gtctcctccg aacgggctgg cgccatgctg ggctggctgg ctgccccgga
     6901 tttagcaacc ggaatgccag cgccgaaagg cgcggctgga ggattcgatt gcgtaaccgc
     6961 gccacgaaca atttacatct gcgcccggcc gcggtgacaa cggttgtcag ccggatggcg
     7021 cccgtcgacc gggcgtgagg ctacgtctcg aagttgatcc atggatccgg cgccgcgagg
     7081 atctcgcaca gccgctggag cgggccggcg aagtgctcgc cggcgttctc gagcacgaac
     7141 cgatccgccc cggccttgaa gggggcgccg cggagggtgc gggcgacggt ctcgcgccac
     7201 gcttcgccgg ccgagcgagg gtcgtccggc ccgccggccc cgaagcggtg gaggtgagtg
     7261 ccccgcgcga tcagctcctg gatggcgccc gtcgctttcg cccggagcca gttccgatcc
     7321 tgctcgcgcc ggatcaggaa ccggtagagg gcgatcgcgc cgatgatccc cgcgacggcc
     7381 gcgtagccga gcagatctcc ctcgtcacgc acgcgcccgg aaagagcaag gcacatgccg
     7441 cgccctcccc ggccgcaaat gcttcagcgg tcgagggggc gcggtcggtc accctatcgg
     7501 tgcggtaaaa ccggtgcggt caatccgacc cgtcgcgtcg ttgctcgacg agccccgctc
     7561 gcgcgtcgct taccgcgcgc ggcgcgtggc cttcgggagc ggcggcttct tccgcttggc
     7621 ctgctccgcc ttcgcgcgcg ccttcacgtc ggcttcctgc ttcccacg
//
LOCUS       DATMCV010000458         3545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_223233, whole genome shotgun sequence.
ACCESSION   DATMCV010000458 DATMCV010000000
VERSION     DATMCV010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223233"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VNU03_10550"
     CDS             <1..463
                     /locus_tag="VNU03_10550"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HWP74948.1"
                     /translation="WKVEGVLARSDPRRKAGDDYAARVYVAFRYDPATASAWERARYW
                     AARLIYGRYPPGLALNYVWESRLPVGTVLDNAYSARAKIVVAQSGAALAGSWVTETHD
                     IYADYRRIVGGGGDGEPPRIEGIALMTDTDDTGERAVAYYDAITLGPGPRQ"
     gene            460..1167
                     /locus_tag="VNU03_10555"
     CDS             460..1167
                     /locus_tag="VNU03_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HWP74949.1"
                     /translation="MRLSVVIPALDEAPNLARLLPDLARACPGAEVIVVDGGSRDGTA
                     AVVTGFAGVRLVEGRRGRARQMNAGAREAGGDVLLFLHADTWLPDGAAAAIAQAVDTP
                     GVVGGRFDVRFDSRRRVLGVVAWLMNARSRATGICTGDQAIFVRRADFEAVGGYPDIP
                     LMEDIDLCRRLKTRGRLAALRDRVTTSARKWEREGPLRTIGLMWALRLLHFCGVAPAR
                     LHRWYYGPRKESHERSH"
     gene            1163..1513
                     /locus_tag="VNU03_10560"
                     /pseudo
     CDS             1163..1513
                     /locus_tag="VNU03_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            1547..1885
                     /locus_tag="VNU03_10565"
     CDS             1547..1885
                     /locus_tag="VNU03_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWP74950.1"
                     /translation="MKRLIALFVAVLFSTGMLATPAVWAQAPKTTPAPAKTDTKPAAK
                     DDKMAHEPLDLNTASEADLKMIPGIGDAYAKKIVQNRPYKRKDELVQKKVLPKATYDK
                     IKDHVVAKQN"
     gene            complement(1854..2762)
                     /locus_tag="VNU03_10570"
     CDS             complement(1854..2762)
                     /locus_tag="VNU03_10570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWP74951.1"
                     /translation="MESRILIIHPGALGDVLQAVPALRGLRAAAPAGRLVFAGQPRLG
                     RLLGALGVVDETRAFDGLGLEALFIDEPAPPALATFLGGFTRVVSWFGSREPTYVTHL
                     GALVAGAIVARPVPDDDTPVWRHLIATLPASALAERDAVTPIRPAARPPAPAALVIHP
                     GSGGTWKQWPAERFAEAIAAVATRHALPVVVHQGPADRAAVEALLARLDSRVERLVEP
                     ELPELAAALSLARAYLGGDSGVSHLAAAVGAPSVILFPPAHLPRFTPWSPTARPIAMT
                     GGADEVAGVVRELERALSSASRRRGP"
     gene            2798..>3545
                     /locus_tag="VNU03_10575"
     CDS             2798..>3545
                     /locus_tag="VNU03_10575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP74952.1"
                     /translation="MKFALFTGMSGTTWDEVLAFWQHCDRTGWDAACVTDHFMPNTAD
                     RVGDTLECWTALAALAARTERLRVGTIVSGNTYRHPALLAKMAANVDIISGGRLICGM
                     GAGWQENEHRAYGMDFYTTRERLERLDEACRVLKALWTEARANYKGRFYRLDDAPLMP
                     KAVQKPHPELMIGGGGEKVTLRIAAQHADHWNVWGGPETLTHKGRILDEHCAKVGRDP
                     KQILRSAVMVLGFADDKAGVERIQQSYMKRM"
BASE COUNT          549 a         1260 c         1308 g          428 t
ORIGIN      
        1 ctggaaggtg gagggcgtgc tcgcccgctc cgacccgcgc cgcaaggcgg gcgacgacta
       61 cgcggcgcgg gtgtacgtgg cgttccgcta cgatcccgcg accgcgagcg cgtgggagcg
      121 cgcgcgctac tgggccgcgc gtctgatcta cggccgctac ccgccaggcc tcgcgctcaa
      181 ctacgtgtgg gagagccggc tgccggtggg caccgtgctc gacaatgcgt actccgcgcg
      241 ggccaagatc gtggtcgcgc agagcggcgc cgccctggcg ggaagctggg tcaccgagac
      301 tcacgatatc tacgccgact atcggcggat cgtcggaggc ggaggcgacg gcgagccgcc
      361 ccgcatcgag ggcatcgcgc tcatgaccga caccgacgac accggcgagc gcgcggtggc
      421 ctactacgac gcgatcacgc tcgggccggg cccgcggcaa tgaggctcag cgtcgtcatc
      481 cccgcgctcg acgaggcccc gaacctcgcg aggctgctgc ccgatctcgc gcgcgcctgc
      541 cccggggccg aggtgatcgt ggtcgacggc ggcagccgcg acgggaccgc cgcggtcgtg
      601 acgggcttcg ccggcgtccg gctcgtcgag gggaggcgcg gccgggcccg gcagatgaac
      661 gcgggggcgc gcgaggcggg cggcgacgtg ctgctcttcc tgcacgccga cacctggctg
      721 cccgacggcg ccgcggccgc gatcgcgcag gccgtcgaca cccccggcgt ggtcggcggc
      781 cggttcgacg tgcgcttcga cagtcgccgg cgcgtcctcg gagtcgtcgc ctggctcatg
      841 aacgcgcggt cgcgcgccac gggcatctgc accggcgacc aggccatctt cgtgcggcgc
      901 gccgacttcg aggcggtggg cggctacccg gacattccgc tgatggagga catcgatctg
      961 tgccggcgcc tcaagacccg cggccgcctc gctgccctcc gcgaccgggt gaccacctcg
     1021 gcccgcaagt gggagcggga aggcccgctc cggaccatcg gcctcatgtg ggcgctgcgc
     1081 ctgctgcact tctgcggggt cgccccggcc cgcctgcacc gctggtacta tgggccgcga
     1141 aaggagagcc atgagcgatc acactgacga tcaggtcgtg cagacggcaa ccggcctcgg
     1201 gtacgtggag ctggtcgagg gcaccggggc ccggccgaag acgggcgaca ccgtgagcgt
     1261 gcactacacc ggctggctca agagcgggac gaagttcgac agctcggtgg accgcggcac
     1321 cccgttcgag ttcccggtgg gcaagggccg cgtcatcaag ggctgggacg agggcgtcat
     1381 ctccatgaag gtgggcggca agcggaagct gatcatcccc gcgcacctgg ggtacggcga
     1441 ccgtggagcg ggcggcgtca tcccgcccgg cgcgaccctc atcttcgagg tggagctgct
     1501 cggcatcggg tgagccgttc acactcacac acgaaaagga gagtccatga agcgattgat
     1561 cgcgctgttc gtcgccgtcc tgttctcgac cgggatgctg gcgaccccgg ccgtctgggc
     1621 ccaggcgccc aagaccaccc cggcgccggc caagaccgac accaagcccg cggccaagga
     1681 cgacaagatg gcgcacgagc cgctggacct caacacggcc agcgaggcgg acctcaagat
     1741 gatcccgggc atcggcgacg cgtacgccaa gaagatcgtg cagaaccggc cctacaagcg
     1801 caaggacgag ctggtgcaga agaaggtgct gcccaaggcc acctacgaca agatcaagga
     1861 ccacgtcgtc gcgaagcaga actgagcgcg cgctcgagct cgcgcaccac gcccgccacc
     1921 tcgtccgcgc cgccggtcat cgcgatcggc cgcgcggtcg ggctccacgg ggtgaagcgt
     1981 ggcaggtgcg cgggcgggaa caggatgacg ctcggcgcgc ccaccgcggc ggcgaggtgg
     2041 ctcaccccgg agtcgccgcc caggtaggcg cgcgcgagcg agagcgcggc ggcgagctcc
     2101 ggcagctcgg gctccacgag ccgctcgacg cgcgagtcca ggcgggcgag cagcgcctcg
     2161 accgccgcgc gatcggcggg gccctggtgg acgacgacag gcagcgcgtg ccgcgtcgcg
     2221 accgccgcga tcgcctccgc gaagcgctcc gccggccact gcttccacgt tccgccgctg
     2281 cccgggtgga tcaccagtgc ggcgggtgcg ggtgggcggg cggccggacg gatcggcgtg
     2341 accgcgtcgc gctccgcgag cgcggaggcg gggagggtcg cgatcagatg ccgccacacc
     2401 ggcgtgtcgt cgtcgggcac cggacgggcc acgatcgcgc cggcgacgag cgcccccagg
     2461 tgcgtcacgt aggtcggctc gcgggagccg aaccacgaga ccacgcgcgt gaagccgccc
     2521 aggaaggtgg cgagcgcggg tggggcgggc tcgtcgatga agagcgcctc gagccccagc
     2581 ccgtcgaagg cgcgcgtctc gtcgaccacg ccgagcgcgc ccagcaggcg cccgagccgc
     2641 ggctggcccg cgaagacgag ccgtccggcg ggcgccgccg cgcgcagccc gcgcagcgcg
     2701 ggcaccgcct ggagcacgtc gccgagcgcg cccggatgga tgatcaggat ccgggattcc
     2761 acttgacctc ccgcgtggcc cctcgtagca tccagccatg aaattcgccc tcttcaccgg
     2821 catgagcggc accacgtggg acgaggtgct ggccttctgg cagcattgcg accggaccgg
     2881 ctgggacgcg gcctgcgtca ccgatcactt catgcccaac accgcggacc gggtgggcga
     2941 cacgctcgag tgctggaccg cgctcgccgc gctcgccgcg cgcaccgagc ggctgcgggt
     3001 cggcaccatc gtgagcggca acacctaccg gcatccggcc ctcctcgcca agatggcggc
     3061 caacgtcgac atcatctcgg gcggccgcct catctgcggc atgggcgcgg gctggcagga
     3121 gaacgagcac cgcgcctacg ggatggactt ctacaccacc cgcgagcggc tcgagcggct
     3181 ggacgaggcg tgccgggtgc tgaaggcgct ctggaccgag gcgcgcgcga attacaaggg
     3241 ccgcttctat cgtctcgacg acgcgccgct gatgcccaag gcggtgcaga agccgcaccc
     3301 cgagctgatg atcggcggcg ggggcgagaa ggtcacgctc cgcatcgcgg cccagcacgc
     3361 cgatcactgg aacgtgtggg gtgggccgga gacgctcacc cacaaggggc ggatcctcga
     3421 cgagcactgc gcgaaggtcg ggcgcgatcc caagcagatc ctgcgctcgg cggtcatggt
     3481 gctgggcttc gccgacgaca aggccggagt ggagcgcatc cagcagtcct acatgaagcg
     3541 catgg
//
LOCUS       DATMCV010000459         4648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_687085, whole genome shotgun sequence.
ACCESSION   DATMCV010000459 DATMCV010000000
VERSION     DATMCV010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687085"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VNU03_10580"
     CDS             complement(<1..312)
                     /locus_tag="VNU03_10580"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWP74953.1"
                     /translation="MDLEALRRRLDAALSRRPRVTLDRSDLVSAAVLVPITDHDGPHV
                     VFTKKTSGVPHHKGQFSFPGGVVETRDGSRVETALREAWEEIRLPAEAVEVLGLLDDT
                     PT"
     gene            complement(320..1087)
                     /locus_tag="VNU03_10585"
     CDS             complement(320..1087)
                     /locus_tag="VNU03_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWP74954.1"
                     /translation="MTASLVEHLLAGRLGRRPVSPLSESTPDLTLERAYALQRDLEQA
                     LIGRGERVIGWKVGFTTAALQERHGVTEPVLGFLLGSGVFGSGDAVPVARFVAPGVEV
                     EVAFLLKADLAGPGVTAASALLAVEGAMPSFELIDFRLSGTPRGVDVIADGVYTNAIV
                     LGRPLTPLAGIDLALEGVVFEQDGQIVATQTAAEVLGNPLVSLAWAANTLGRMGRGLR
                     AGDIVLTGSISKVLRPVAGQSARASFTRLGSVSCRFV"
     gene            complement(1101..1883)
                     /locus_tag="VNU03_10590"
     CDS             complement(1101..1883)
                     /locus_tag="VNU03_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HWP74955.1"
                     /translation="MIPSEVERLADLVSRAGRIVAFTGAGVSTESGIPDFRSPGGVWD
                     RFDPREFTFQNFMGSEAGRRRYWELGRTTFPVIRRAEPNPGHQALVTLWRLGKLDCVI
                     TQNIDNLHQRAGLPADRVIELHGNATRARCLTCETAYERERVQEWLDAGVAIPMCDPP
                     CGGIIKPRTVMFGEAMPRVELEEAERRARASDLFVVVGSSLVVYPAAYMPLHAKDAGA
                     TLAIVNLTETPHDGQADLLVRGSAAGVLAAVVDRLQPRPVAR"
     gene            complement(1880..2239)
                     /locus_tag="VNU03_10595"
     CDS             complement(1880..2239)
                     /locus_tag="VNU03_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74956.1"
                     /translation="MPELTRRYIVMTMATEAPFDTQDPEAPFVLKPWKDPAALRALET
                     YRDHAYPELAADLAAWIKAIASGPRMRGGVGTRNEAHLASRPKTRPAAARPKAKPKPA
                     KKRPARKAVKAKKKRRS"
     gene            complement(2461..3741)
                     /locus_tag="VNU03_10600"
     CDS             complement(2461..3741)
                     /locus_tag="VNU03_10600"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HWP74957.1"
                     /translation="MIPIIAVSFAVLLLLGIPVAFVIGVAGFIGLWWSGEYPLTVIVK
                     QIFEGVDSFVLLAIPLFILAGAFMETGGIAVRLVRLAQVLVGWIRGGLSMAVVVAEYI
                     FSGISGSTVADVSAIGSTMIPPMLRAGYRPEQAVAVVSAASAMGILVPPCILMIVIGS
                     VANVSVAALFAAGFLPAVVLALAIMAYIWYDAGRSGIEARARASWGDIGRAFVDAVIP
                     LGLPAIIFGGILGGVVTPTEAAVLAVIYAFIVGVFVYREIAWADLPRILVHSAVVTAA
                     VCFLLGTAAVVAWVLAVQQIPQLLLKAMLAVPGGNLVFLALTALLFIVLGAVLEGLPA
                     VVILLPTFMPVVSRLGIDVIHYSTVVVAATGIGLFLPPIGVGFFIACGIANIPGDRAT
                     RAMMPYVYMMCVGLLIVILVPWVTLVVPRLLGLG"
     gene            complement(3738..4211)
                     /locus_tag="VNU03_10605"
     CDS             complement(3738..4211)
                     /locus_tag="VNU03_10605"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HWP74958.1"
                     /translation="MARALSQVVEGLLLVMMVVLCADVFLGVFSRYVMARTFTWYDEI
                     ARLLFVWIVFLGAAVGVRRSAHFRLHLLVDRFPPGLRRVAHVIGVLVVMGFGLLLIQQ
                     GWKLVELGQFQRTPVMGLSKQYVYLSVPVGGALIMLYSLPHLWRALRVVPGPAAA"
     gene            complement(4218..>4648)
                     /gene="dctP"
                     /locus_tag="VNU03_10610"
     CDS             complement(4218..>4648)
                     /gene="dctP"
                     /locus_tag="VNU03_10610"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HWP74959.1"
                     /translation="PMPYVEVYLAMKQGTIDGMELPSFTFTSDKFQEVTKYYSLTRHS
                     YPPIAWFMNLKKYQALPGDVQKVVADSMKDACQLHRRLEVEKEKNDFDVMKKAGVQIN
                     DVKDIRAFQALMKPAYDYVEGQVGKEFMTRLRAAVQAASK"
BASE COUNT          768 a         1651 c         1621 g          608 t
ORIGIN      
        1 cgtcggggtg tcgtcgagca ggcccagcac ctccaccgcc tcggcgggca gccggatctc
       61 ctcccacgcc tcgcgcagcg cggtctcgac tcgcgagccg tcgcgggtct ccaccactcc
      121 acccgggaac gagaactgcc ccttgtggtg gggcacgccg gacgtcttct tggtgaagac
      181 cacgtgggga ccgtcgtgat cggtgatcgg caccagcacc gcggcggaga ccagatcgct
      241 gcggtcgagg gtcacgcgcg ggcggcggct cagcgccgcg tcgaggcgtc tccgcagggc
      301 ctccaggtcc atccggcgat cagacgaacc ggcacgacac gctgccgagg cgggtgaagg
      361 aggcgcgcgc ggactggccg gcgacgggac gcagcacctt cgagatggag ccggtcagca
      421 cgatgtcccc cgcgcgcagg ccccggccca tgcggccgag cgtgttggcg gcccacgcga
      481 gcgacaccag cgggttgccc agcacctcag ccgcggtctg ggtggcgacg atctggccgt
      541 cctgctcgaa caccacgccc tcgagggcca ggtcgatgcc cgcgagcggc gtgagcggcc
      601 ggcccagcac gatggcgttg gtgtacacgc cgtccgcgat cacgtcgacg ccgcgtgggg
      661 taccgctcag gcggaagtcg atcagctcga acgacggcat cgcgccctcg accgcgagca
      721 gcgcggaggc ggcggtcacc cccggtccgg ccaggtcggc cttgaggagg aacgcgacct
      781 cgacctcgac gccgggcgcg acgaagcgcg cgacggggac cgcgtccccg ctcccgaaga
      841 cgccggagcc gagcaggaag cccagcaccg gctcggtcac cccgtgtcgc tcctggagcg
      901 cggcggtcgt gaagcccacc ttccatccga tgacgcgctc gccgcgcccg atcagggcct
      961 gctcgagatc tcgctggagg gcatacgcgc gctcgagcgt gagatccgga gtggattcgc
     1021 tcagcgggct caccgggcgg cggcccagcc ggcccgccag gagatgctcg acgagagagg
     1081 cggtcaccgc gcggcccggc tcagcgggcc accggccgcg gctgcagccg atcgacgacg
     1141 gcggccagga cccccgcggc gctcccgcgc accagcaggt cggcctggcc gtcgtgcggg
     1201 gtctcggtca ggttcacgat cgcgagggtg gcgcccgcat ccttggcatg gagcggcatg
     1261 taggcggcgg ggtagacgac gagggacgag cccaccacca cgaacaggtc gctggcccgg
     1321 gcccggcgct ccgcctcctc cagctccacc cgcggcatcg cctcgccgaa catgaccgtg
     1381 cgcggcttga tgatgccccc gcagggtggg tcgcacatcg ggatcgccac gcccgcgtcg
     1441 agccactcct ggacccgctc ccgctcgtac gccgtctcgc aggtgaggca gcgcgcgcgg
     1501 gtagcgttgc cgtgcagctc gatgacccga tcggccggga gccccgcccg ttggtggaga
     1561 ttgtcgatgt tctgggtgat gacgcagtcg agcttgccga gccgccacag ggtcaccagg
     1621 gcctggtgtc cgggattcgg ctcggcccgc cggatcaccg ggaaggtggt gcggccgagc
     1681 tcccagtagc ggcgccggcc ggcctcgctg cccatgaagt tctggaaggt gaactcgcgg
     1741 ggatcgaagc ggtcccagac cccgcccggg ctgcggaagt cggggatgcc cgactcggtg
     1801 ctgacgccgg cgccggtgaa cgcgacgatg cggccggctc tcgagacgag atcggccagc
     1861 cgctcgacct cggatggaat cacgagcggc gcttcttctt cgctttcacg gctttcctgg
     1921 ccggcctctt cttcgccggt ttaggcttcg ccttgggccg ggcggcggcc ggacgggtct
     1981 tcggtcggct ggccaggtgc gcctcgttgc gggttccgac gccgccccgc atccggggtc
     2041 cgctcgcgat ggccttgatc cacgcggcga gatcggcggc gagctcgggg taggcgtggt
     2101 cgcggtaggt ctcgagcgcg cggagggcgg ccgggtcctt ccacggcttc agcacgaacg
     2161 gcgcctccgg atcctgcgtg tcgaagggcg cttcggtggc catggtcatc acgatgtaac
     2221 gccgggtcag ctctggcatg tcgatcgcct cgcgtcgctc gagggggccg ccgtcggccc
     2281 gcccgtcgcg cgctcagggc tgggagagaa gcgcgaccgc ggaaaccgat ccggggccgc
     2341 ccaggttgtc cgtcagtccg atatcgcgga tcatcaccga cttctccttc gagcccatcg
     2401 cccgctcgag ccgtccaagc gtactctggg gtcggccgga cctgcaagcc tcccgcccgg
     2461 tcagccgagc ccgagcagcc gcgggaccac cagggtgacc cacggcacca ggatcacgat
     2521 caggagtccc acgcacatca tgtagacgta gggcatcatc gcgcgggtcg cgcggtcgcc
     2581 cgggatgttg gcgatcccgc acgcgatgaa gaagccgacg ccgatcgggg gcaggaacag
     2641 cccgatgccg gtggccgcga ccaccaccgt gctgtagtgg atcacgtcga tgccgagccg
     2701 gctcaccacc ggcatgaagg tcgggagcag gatcaccacc gcgggcagcc cttccagcac
     2761 cgcgcccagc acgatgaaca gcagcgcggt cagggcgagg aacacgaggt tgccgccggg
     2821 caccgcgagc atcgccttca gcaggagttg cgggatctgc tgcaccgcga gcacccacgc
     2881 gaccaccgcg gcggtgccga ggaggaagca caccgcggcg gtcaccaccg cgctgtggac
     2941 gaggatccgg ggcaggtcgg cccaggcgat ctcgcggtac acgaagacgc cgacgatgaa
     3001 cgcgtagatc accgcgagca ccgcggcttc ggtcggggtc accaccccgc ccaggatgcc
     3061 gccgaagatg atggcgggca ggccgagcgg gatcaccgcg tcgacgaagg cccgcccgat
     3121 gtcgccccag gaggcccgcg cccgcgcctc gatgccgctg cggcccgcgt cgtaccagat
     3181 gtaggccatg atcgcgagcg ccaggaccac cgcgggcagg aagccggccg cgaacagggc
     3241 ggccaccgag acgttggcca ccgagccgat cacgatcatg aggatgcagg gtggcaccag
     3301 gatgcccatc gcggaggcgg ccgagaccac cgccaccgcc tgctccggcc ggtagcccgc
     3361 gcgcagcatc ggcgggatca tggtggagcc gatggcggac acgtcggcca ccgtggagcc
     3421 ggagatgccg gagaagatgt actccgcgac caccaccgcc atcgagagac cgccccggat
     3481 ccaccccacc agcacctggg cgagccggac cagccgcacc gcgatgccgc cggtctccat
     3541 gaaggcgccg gccaggatga agagcgggat cgcgagcagc acgaacgagt ccacgccctc
     3601 gaagatctgc ttcacgatca cggtgagcgg gtactccccg ctccaccaga ggccgatgaa
     3661 gccggccacc ccgatgacga aggccaccgg gatcccgagc aggagcagga ccgcgaagga
     3721 gaccgcgatg atcgggatca tgccgcggcc ggtcccggca cgacgcggag ggcgcgccag
     3781 aggtgcggca ggctgtagag catgatcagg gcgccgccca ccggcaccga caggtacacg
     3841 tactgcttgg agaggcccat caccggcgtg cgctggaact ggcccagctc caccagcttc
     3901 cagccctgct ggatcaggag caggccaaag cccatcacga ccagcacccc gatcacgtgg
     3961 gcgacgcgac ggaggccggg cggaaagcgg tcgaccagga gatgcaggcg gaaatgggcg
     4021 ctgcgccgca cccccaccgc cgcgcccagg aacacgatcc acacgaagag gaggcgggcg
     4081 atctcgtcgt accaggtgaa cgtgcgggcc atcacgtagc gggagaagac cccgagaaac
     4141 acgtccgcgc acagcaccac catcatcacc agcagcaggc cctcgacgac ctgggacagc
     4201 gcccgggcca ccacggacta cttcgacgcc gcctggaccg ccgcccggag ccgggtcatg
     4261 aactccttgc ccacctgccc ttcgacgtag tcgtaggccg gcttcatgag ggcctggaac
     4321 gcccggatgt ccttcacgtc gttgatctgg acccccgcct tcttcatgac gtcgaagtcg
     4381 ttcttctcct tctcgacctc gagccggcgg tgaagctgac aggcgtcctt catcgagtcg
     4441 gccaccacct tctggacgtc gccgggaaga gcctggtact tcttgaggtt catgaaccag
     4501 gcgatcggcg gatacgaatg ccgggtcagc gagtagtact tggtcacttc ctgaaacttg
     4561 tccgaggtga acgtgaacga cggcagctcc atcccgtcga tcgtcccctg cttcatcgcg
     4621 aggtacacct cgacgtaggg catcggca
//
LOCUS       DATMCV010000460         4139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_687093, whole genome shotgun sequence.
ACCESSION   DATMCV010000460 DATMCV010000000
VERSION     DATMCV010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687093"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..990)
                     /locus_tag="VNU03_10615"
     CDS             complement(43..990)
                     /locus_tag="VNU03_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP74960.1"
                     /translation="MSVTIEASRARQDGATESYHQCWFPVALASDVPAGRPLGRDFLG
                     TRVVVYRDATGRAVVQGAYCPHLGADLSVGEVAEGQIRCAYHHWRFDCAGTCVDIPAG
                     DRIPPGARIPTYPTAEAWGLIWAFNGEAPTYPVPGIPGVEERELICETHYRGTRGIDP
                     WVSISNGVDFQHLRTLHGLSAVDPDAVAVGPHSLEYRIESPHFIQHGRITGVNVFAQH
                     LSMAGQDVFMLFSGAPIEHGRTMGFYAYGVRDDGTGRDGAAERLGTLRGFVQRLLAED
                     APVLDTIRFRPRNLVASDRHLARFFKYVREFPRAIPPGA"
     gene            1094..2095
                     /locus_tag="VNU03_10620"
     CDS             1094..2095
                     /locus_tag="VNU03_10620"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP74961.1"
                     /translation="MRSIDIHAHLVPQTLWQAATVGREWYGFRHEPGEGLGTFLGTNG
                     KRTGFTSPKVRLTPEERLEDMDTQGVDMQVISIHTPLFSYHLEPAQGLALAREVNDEI
                     AAMTRQWPTRFAGLATLPVQDVAAAIGELERAVAVLGLKGAELDTIVNGENWDEPRFL
                     PLFKAAEAMGAVLFFHPQPQHNFMVERAGRYGLFNSLGVIVEDAIVVAVLILGGILEA
                     CPGLKACIAHGGGPACYAMGRLDRGWQGTPAARAHTPSPPSSYQKRLYYDTVVGNEAA
                     LRFLLDQVGADRVVLGSDWPFVPWHPSPVAWLQGLASITATEKEQILYRNLEALLRL"
     gene            2137..3063
                     /locus_tag="VNU03_10625"
     CDS             2137..3063
                     /locus_tag="VNU03_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWP74962.1"
                     /translation="MPKRYDYVTVDVFTDRAFAGNPLAVVIDAAGLDTAQMQAIAAEF
                     NYSESTFVLPPENPSHTARVRIFTRQNEIPFAGHPNVGTAFVLGRLAERKGAPLGATV
                     RFEEAAGLVPVDLERDGAGHVVGATLTAPQPLALDQTIPAAVIAECVGLEAAAVLTGS
                     HEPVVASVGLPFVLAEVAADAIGRAVSDAVATASAARRYAFRSGRFSVHIYARTPGRA
                     DGADLRARMFSPLGGTGEDPATGSANGALIALLATLDPAADTVLALDVLQGAEMGRPS
                     YLRVTAEKQAGAVTRVRVGGRCAPVMEGALTV"
     gene            complement(3067..3471)
                     /locus_tag="VNU03_10630"
     CDS             complement(3067..3471)
                     /locus_tag="VNU03_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP74963.1"
                     /translation="MSARKATKAVNGKGTNGKATRGAGGGNTYGYPFWEWVAREDPEY
                     VKARQPLSQLSIGEGRELSVKYREMVIIGILAFRGRKDGVIAHMRRAIDHGATKRELL
                     EAIQSAAVPGGGPCFSTGAQALMELDRAGVFR"
     gene            complement(3489..>4139)
                     /locus_tag="VNU03_10635"
     CDS             complement(3489..>4139)
                     /locus_tag="VNU03_10635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP74964.1"
                     /translation="AAHTSRILLATGVIILPQRQAVVLAKQLASLDVLSNGRLIFGLG
                     VGWCEPEMRSVGAPFKERGRVSDDYLASMRAVWTQPKPAYHGPYVSFESVQAMPRPVQ
                     SPIPIVVGGGTPPAYRRAVAQGHGWYGFGLDVAETRKRVGALQQAASQVKRPAELGPL
                     EISVTPPGYDVPDRATIEAYTAAGVHRLILRPQPTLDGPGLERFAAETGRTLGLAA"
BASE COUNT          588 a         1518 c         1447 g          586 t
ORIGIN      
        1 aactcttcga cctgctcggc ggacagggtc cgcggcatgg cctcaggctc ccggcgggat
       61 cgcgcgcggg aactcgcgca cgtacttgaa gaagcgggcc agatgccggt cggaggccac
      121 caggttgcgc ggacggaacc ggatggtgtc gagcaccggc gcgtcctccg cgagcaggcg
      181 ctgcacgaag ccgcgcagcg tcccgagccg ctcggccgcc ccatcgcgac ccgtcccgtc
      241 atctcgcacg ccgtaggcgt agaatcccat ggtccggccg tgctcgatcg gcgccccgct
      301 gaagagcatg aagacgtcct ggccggccat cgacaggtgc tgcgcgaaca cgttcacccc
      361 ggtgatgcgg ccatgctgga tgaagtgagg ggactcgatg cggtactcga gcgagtgcgg
      421 acccaccgcc accgcgtcgg ggtccaccgc ggagaggccg tgcagggtgc ggaggtgctg
      481 gaagtcgacc ccgttcgaga tcgacaccca gggatcgatg ccgcgggtgc cgcgatagtg
      541 cgtctcgcag atcagctccc gctcctcgac gccgggaatt ccaggcacgg ggtaggtcgg
      601 cgcttctccg ttgaaggccc agatcaggcc ccacgcctcc gcggtcggat aggtcgggat
      661 gcgggccccg ggcgggatgc ggtcgccggc gggaatgtcg acgcacgtcc ccgcgcagtc
      721 gaaccgccag tgatggtagg cgcagcggat ctggccctcc gccacctcgc ccaccgagag
      781 gtcggcaccg agatgggggc agtacgcccc ctgtaccacc gcgcggccgg tcgcgtcgcg
      841 atagaccacg acgcgcgtgc ccaggaaatc ccggcccagc ggacgaccgg caggcacgtc
      901 gctcgcgagg gccaccggga accagcactg gtgatagctc tcggtcgccc cgtcctgccg
      961 ggcccgactt gcctcgatcg tcacgctcat ggcttgcctc ctctgaatcg gtcggccaat
     1021 gaattgacac tgtatattta caatgtaagt cagtcaaccc tttcttctac ctcttcgacc
     1081 cgggagatca gccatgagat cgatcgacat ccacgctcat ctggtgccgc agaccctctg
     1141 gcaggccgcc accgtgggcc gggagtggta cggcttccgg cacgagcccg gcgaggggct
     1201 cggcacgttc ctcggcacca acggcaagcg taccggcttc acctctccca aggtgcgcct
     1261 gaccccggag gagcggctcg aggacatgga cacgcagggc gtggacatgc aggtgatctc
     1321 catccacact ccgctcttca gctaccacct ggaaccggcg cagggcctgg ccctggcccg
     1381 cgaggtcaac gacgagatcg cggcgatgac ccgccagtgg cccacgcgct tcgcggggct
     1441 cgccaccctg ccggtgcagg acgtggcagc ggccatcggc gagctcgagc gcgcggtcgc
     1501 cgtgctcggc ctcaaggggg cggagctcga cacgatcgtc aacggggaga actgggacga
     1561 gccgcgcttc ctgccgctct tcaaggcggc ggaggccatg ggcgcggtgc tcttcttcca
     1621 cccacagccc cagcacaact tcatggtcga gcgcgccggc cgctacggcc tcttcaatag
     1681 cctgggggtg atcgtggagg acgcgatcgt ggtcgcggtg cttatcctgg gcggcatcct
     1741 cgaggcctgc ccgggcctca aggcctgcat cgcccacggc gggggccccg cgtgctacgc
     1801 gatgggccgc ctcgaccgtg gctggcaggg cacaccggcg gcgcgggccc acaccccctc
     1861 gccgcccagc agctaccaga agcggctcta ctacgacacg gtggtgggca acgaggccgc
     1921 gctccgcttc ctgctcgacc aggtcggcgc cgaccgggtc gtcctcggca gcgactggcc
     1981 cttcgtgccg tggcatccct cgccggtcgc gtggctccag ggcctcgcca gcatcaccgc
     2041 cacggagaag gagcagatcc tctaccgaaa cctggaagcg ctgctgcggc tctagccggc
     2101 tgcccccctg acactgcgcc cccgatagga gaccgcatgc cgaagcgcta cgactacgtg
     2161 accgtcgacg tgttcaccga ccgcgcgttc gcgggcaacc cgctggcggt cgtgatcgac
     2221 gcggccgggc tcgacaccgc gcagatgcag gccatcgcgg ccgagttcaa ctactcggag
     2281 tcgacgttcg tgctgccgcc cgagaacccc agccacaccg cgcgggtccg gatcttcacg
     2341 cggcagaacg agatcccgtt cgccggccat cccaacgtgg gcacagcctt cgtgctgggc
     2401 cggctcgccg agcgcaaagg cgccccgctc ggcgcgaccg tccgcttcga ggaggcggcg
     2461 gggctggtgc cggtggatct cgagcgggac ggcgcgggac acgtggtggg ggccacgctc
     2521 accgcccccc agccgctcgc cctcgaccag acgatccccg ccgccgtgat cgccgagtgt
     2581 gtcggcctgg aggccgccgc cgtgctcacg ggatcgcacg agccggtggt ggcctccgtg
     2641 ggcctgccgt tcgtactggc ggaggtggcg gcggacgcga tcggccgggc ggtgtccgac
     2701 gcggtcgcga ccgcctccgc ggcgcgccgc tacgcgttca ggtccggccg cttctccgtc
     2761 cacatctacg cgcgcacgcc gggacgggcg gacggcgccg atctccgggc gcgcatgttc
     2821 tcgccgctgg gcggcaccgg ggaggatccc gccaccggca gcgccaacgg agcgctgatc
     2881 gccctgctgg cgacgctcga ccccgccgcg gacaccgtgc tggcgctcga cgtgctgcag
     2941 ggggcggaga tgggccgccc gagctacctg cgggtcaccg ccgagaagca ggcgggcgcc
     3001 gtcacccgcg tccgggtcgg tgggcgctgc gccccggtga tggagggcgc gctcaccgtc
     3061 tgagcgctag cggaagacgc cggcgcgatc cagctccatc agcgcctgcg ccccggtgct
     3121 gaagcagggg ccgccgcccg ggaccgcggc cgactggatc gcctccagca gctcgcgctt
     3181 ggtcgccccg tggtcgatgg cccggcgcat gtgggcgatc acgccgtcct tgcggccgcg
     3241 gaaggcgagg atgccgatga tcaccatctc gcggtacttc accgacagct cgcggccctc
     3301 gccgatggag agctggctga gcggctggcg agccttcacg tactccgggt cctcccgcgc
     3361 cacccattcc cagaacgggt acccgtaggt gttcccgccg ccggcgccgc gcgtcgcctt
     3421 gccgttggtc cccttgccgt tcaccgcctt cgtcgccttg cgtgcgctca tggggtgcct
     3481 cctccggctc aggccgcgag cccgagcgtg cggccggtct cggcggcgaa gcgctcgagg
     3541 cccggcccgt ccagcgtggg ttgtggccgc aggatcagcc ggtggacgcc ggccgccgtg
     3601 taggcctcga tcgtcgccct gtccggcacg tcgtagccgg gcggggtcac gctgatctcg
     3661 agcggcccca gctccgccgg ccgcttcacc tgactcgcgg cctgctggag cgcgcccacg
     3721 cgcttgcggg tctccgccac gtccaggccg aagccgtacc agccgtggcc ctgggcgacc
     3781 gcgcggcgat aggccggcgg cgtgcccccg cccaccacga tcgggatcgg cgactgcacc
     3841 ggccggggca tggcctgcac gctctcgaac gacacgtagg gcccgtggta cgccggctta
     3901 ggctgcgtcc acaccgcacg catcgaggcc aggtagtcgt cggagacgcg gccgcgctcc
     3961 ttgaacgggg cgcccaccga gcgcatctcc ggctcgcacc agccgacgcc caggccgaag
     4021 atgagccggc cattcgagag cacgtcgagc gacgcgagct gcttggcgag caccaccgcc
     4081 tggcgctggg gcaggatgat gacgcccgtg gcgagcagga tgcgcgaggt gtgggcggc
//
LOCUS       DATMCV010000461         2344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_687170, whole genome shotgun sequence.
ACCESSION   DATMCV010000461 DATMCV010000000
VERSION     DATMCV010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687170"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VNU03_10640"
     CDS             complement(<1..486)
                     /locus_tag="VNU03_10640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWP74965.1"
                     /translation="MTAPPLFDTHAHLHFPELVADLDGVLERARAAGVTGMVTIGTDR
                     ETNPAAVALAERLDAVHATVGIHPHDAAEATPADFEAMEALARGSAKVVALGEMGLDF
                     FRNLSPPDIQAAVFRRQLAMARDLGKPVVIHCRDAHAEALAILAEERVSEVGGVMHCF
                     SA"
     gene            complement(483..1790)
                     /locus_tag="VNU03_10645"
     CDS             complement(483..1790)
                     /locus_tag="VNU03_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWP74966.1"
                     /translation="MDSIWVEIFLIGVSILANAFFAGSEIALVSARPSRLSQLRAHGV
                     PGAEIALTLKKDPDAFLATIQMAITLVGTLASAVGGAAGAEALTPRLQALGLPGAWGR
                     SVALAIVVLAIAFVSLLIGELTPKALALRNPERLAALVARPVAGLMRLLAGPGRGLTW
                     ATRLILTVIGQSDAPPASLVSEEEVKYLVREGTTQGIFDVAERDLVNRVFQFADTPVR
                     LVMVPRPNILGLDIETPPAEVLMRAARLGHTRLPVFRGSIEDARGVVVIKDLFRCAAL
                     AQPVNLERLMHPPLFVPESAPVSAVLRQFQQHRLHLALVVDEYGQVAGLVTTEDLLEE
                     IVGEIRDERESSHLQSVSRLPDGSYMIDGTATIRDLREQVGLPLEESPDYQTVAGFLL
                     HRLGAVPRPGTMVVAGGYRWTIVEMDGPRIMKARAEPERPDTR"
     gene            complement(1801..>2344)
                     /locus_tag="VNU03_10650"
     CDS             complement(1801..>2344)
                     /locus_tag="VNU03_10650"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HWP74967.1"
                     /translation="VGVRPIMALEGGAVLAALALGPAFRRSGAATWWRAHARALGAAV
                     VALGLVWAGRILVPPAPLFLAHAVVARGVSEMQPLEPAEDGRLSAATVLEWGEVAAYT
                     AVYAPAGVRQAIGHVWFRDRQRVAYVALPTPVQGGRTQGFRTYSRYTELKAPIPGRYR
                     VDVITASGQLIGRLRFTVTP"
BASE COUNT          336 a          848 c          881 g          279 t
ORIGIN      
        1 ggccgagaag cagtgcatga ccccgcccac ctcggagacc cgctcctcgg ccaggatcgc
       61 gagggcctcg gcgtgcgcgt cgcggcagtg gatcaccacc ggcttgccga gatcccgcgc
      121 catggcgagc tggcggcgga agacggcggc ctggatgtcg ggcggcgaga ggttgcggaa
      181 gaagtcgagc cccatctcgc cgagcgccac caccttcgcg gagccgcgcg cgagcgcctc
      241 catcgcctcg aagtcggccg gggtggcctc cgccgcgtcg tgggggtgga tgcccacggt
      301 ggcgtgcacg gcgtcgaggc gctccgcgag cgccaccgcg gcgggattgg tctcccggtc
      361 ggttccgatc gtcaccatgc cggtcacgcc agccgcgcgc gcccgctcga gcacgccgtc
      421 caggtccgcg acgagctccg ggaagtgcag gtgcgcgtgg gtgtcgaaga gcggcggggc
      481 ggtcatcgcg tgtcgggcct ctcgggctcg gctctcgcct tcatgatgcg cgggccgtcc
      541 atctccacga tcgtccaccg gtagccgccc gcgaccacca tggtgccggg ccgcgggacc
      601 gcgccgaggc ggtgcagcag gaagcccgcg acggtctggt agtcgggcga ctcctcgagc
      661 ggcagcccga cctgctcgcg caggtcgcgg atggtcgcgg tcccgtcgat catataggac
      721 ccgtccggca ggcgggacac ggactgcagg tgcgagctct cgcgctcgtc ccggatctcg
      781 cccacgatct cctcgagcag gtcctcggtg gtgacgaggc cggcgacctg accgtactcg
      841 tcgaccacca gggccaggtg cagtcggtgc tgctggaact ggcgcagcac cgcgctcacc
      901 ggcgcgctct ccgggacgaa cagcggcggg tgcatgaggc gctcgaggtt caccggctgg
      961 gcgagcgccg cgcagcggaa caggtccttg atcaccacga ccccccgcgc gtcctcgatg
     1021 gagcctcgga agacgggcag gcgcgtgtgg ccgagccggg ccgcacgcat gagcacctcc
     1081 gcgggcggcg tctcgatgtc gaggcccagg atgttcgggc gcggcaccat caccaggcgc
     1141 accggggtgt cggcgaactg gaacacgcgg ttgaccaggt cgcgctcggc gacgtcgaag
     1201 atgccctgcg tggtgccctc gcggaccagg tacttcacct cctcctcgga gaccagcgag
     1261 gcgggcggcg cgtccgactg gccgatcacg gtgaggatga ggcgggtcgc ccacgtgagg
     1321 ccgcgccccg gccccgcgag caggcgcatc agccccgcga ccggccgggc caccagcgcg
     1381 gccagccgct ccgggttgcg cagggccagc gccttggggg tcagctcacc gatcagcagc
     1441 gacacgaacg cgatcgcgag cacgacgatc gccagcgcga ccgatcggcc ccacgcgccg
     1501 ggcagcccga gcgcctgcag ccgtggcgtg agggcctccg cgccggcggc gcctcccacc
     1561 gcggaggcga gcgtgccgac cagggtgatc gccatctgga tggtggcgag gaacgcgtcg
     1621 ggatccttct tgagggtgag ggcgatctcc gcccccggca cgccgtgggc ccggagctgg
     1681 ctcagacgcg agggacgcgc cgagaccagc gcgatctcgg agccggcgaa gaacgcgttg
     1741 gccaggatcg agacgccgat gaggaagatc tccacccaga tgctgtccat cgtccacccc
     1801 tcagggagtc acggtgaacc gcagtcggcc gatgagctgg ccggaggcgg tgatgacgtc
     1861 gacgcgatag cggcccggga tcggggcctt gagctcggtg tagcgcgagt aggtgcggaa
     1921 gccctgcgtg cgacccccct gcaccggcgt cggcagagcc acgtacgcga cccgctgacg
     1981 gtcgcggaac cagacatgtc cgatggcctg gcgcaccccg gccggcgcgt acaccgcggt
     2041 gtaggctgcc acctcgcccc actcgagcac ggtcgcggcc gagagccggc cgtcctcggc
     2101 gggctcgagc ggctgcatct cgctgacgcc gcgcgccacc accgcgtggg cgaggaagag
     2161 cggcgcgggc gggacgagga tgcggccggc ccacaccagg ccgagcgcga ccaccgccgc
     2221 gccgagcgcg cgggcgtggg cgcgccacca ggtcgcggcg ccgctccggc ggaaggcggg
     2281 gccgagggcc agcgcggcga gcaccgcgcc gccctcgagg gccatgatcg gccgcacccc
     2341 gacc
//
LOCUS       DATMCV010000462         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_223368, whole genome shotgun sequence.
ACCESSION   DATMCV010000462 DATMCV010000000
VERSION     DATMCV010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223368"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VNU03_10655"
     CDS             complement(<1..450)
                     /locus_tag="VNU03_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74968.1"
                     /translation="MHGLRRVLAGLGRALHARRGTFAGVAATVFALDILVPPVVLSVA
                     RARVDYFTFNPWLPSLPGYLRAGPGSLGERLDRAFNLALFWFSADGIFGVDWGFAVTA
                     ADLVRFGVMAILVGAYFALWRDRRGPPGSAGRGLQVAGQGGVLGAAGS"
     gene            594..1979
                     /locus_tag="VNU03_10660"
     CDS             594..1979
                     /locus_tag="VNU03_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP74969.1"
                     /translation="MTQEVSRRDFLARLGVTVGTAAAGAAAAAGVPLAAVPLVSPAYG
                     QPKGTVPDTPYKIAHMTFFTGPAAVLGEPSFKGHTLAADEINAQGGLLGKRKIETLKA
                     DENAGTDANVKEMRRLKLSEKVDLFTGVISSGNTPALGPVAEELKLLTIFVDGCTDFL
                     FDKAVPNPHYIFRITNIQSADGVTCGLAAAMTWGKQIKRIAHIHPDYSYGRNAFDHFN
                     IVMKKMVPGAQTVSEGWPKLGTTDFTSHITKAISSKPDLLVSSVWGGDYVALYKQALR
                     YDMFKKMKFASTIAFGVAPHAIGKDHPEGAIAGVHSNYYFNYPPNNRWPLNTEFVKKY
                     YDRWKEYPNFQSEGAYSTLYMLKTAVEKANKLAGGWPDDDAIISQLEGLMHPGPAGYV
                     YIRPDTHQGYKDAMTGFSINSPDYPFQILDPSRIITIPIRNITAPPGWPKGEPTSTYT
                     WIDKTWPQMKA"
     gene            2036..>2805
                     /locus_tag="VNU03_10665"
     CDS             2036..>2805
                     /locus_tag="VNU03_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74970.1"
                     /translation="MRSFTALASALAALALLAAPARAGHELPFYPGYYPQEIRLETLT
                     PAAAVPLLRRSTLHAYVGADPFGGGRLPANVSAVESLAGYVVATPGAATAGADRTAAR
                     CAGALAVARAVGAARVAGFVAHPYAVTPYHGDYLEHFDLAEASRRAVEGPAAGVPPRL
                     LPRGPLAERLAPASKGGSAAKGDAMDVEEIDLQSLLAPYIVNAGGSFGPPWLKEGWFH
                     AYLLQASTMTDATARQAADQLFHRLVTGAYEGPAERADL"
BASE COUNT          460 a         1046 c          923 g          376 t
ORIGIN      
        1 gctcccggcc gctcccagca cgccgccctg cccggccacc tggagtcccc ggccggcgct
       61 cccgggcggg ccgcgccggt cgcgccagag cgcgaagtag gcgccgacca gaatcgccat
      121 caccccgaac cggaccagat cggccgcggt caccgcgaac ccccagtcga ccccgaagat
      181 cccgtccgcc gagaaccaga agagcgccag gttgaaggca cgatcgagcc gctcgccgag
      241 ggatccgggg cccgcccgga ggtagccggg caggctgggc agccacggat tgaaggtgaa
      301 gtagtcgacc cgcgcgcggg cgaccgagag gaccaccggg gggacaagga tgtcgagggc
      361 gaacacggta gccgccacgc ccgcgaaggt gccgcgccgc gcgtggagcg ctcggccgag
      421 acccgcgagg acgcgcctga gcccgtgcat ggtccgccgg ggccgattat agagggagcg
      481 cggaagttcg gggaacttct tgcgctggcg cgggttgctg gggcagtatg gcgctgcatg
      541 acccccgcgc tgcaatgtcg ttcgagcaga cccccaacga ggagggccca cacatgactc
      601 aagaggtctc ccgccgtgac ttcctggccc gtctgggtgt gaccgtcggc accgccgccg
      661 ccggcgccgc cgccgccgcc ggggtgcccc tggccgccgt tcccctggtc agcccggcgt
      721 acgggcagcc caagggtacc gtgccggaca ctccgtacaa gatcgcccac atgacgttct
      781 tcaccgggcc ggcggcggtc ctgggcgagc cctcgttcaa ggggcacacg ctggccgccg
      841 acgagatcaa cgcccagggc ggcctgctcg gcaagcgcaa gatcgagacc ctcaaggccg
      901 acgagaacgc gggcaccgac gccaacgtga aggagatgcg gcggctcaag ctctccgaga
      961 aggtcgacct cttcaccggc gtcatctcga gcggcaacac ccccgcgctc ggtccggtgg
     1021 ccgaggagct gaagctgctc acgatcttcg tggacggctg caccgacttc ctgttcgaca
     1081 aggcggtgcc gaacccccac tacatcttcc gcatcaccaa catccagtcg gcggacgggg
     1141 tcacctgcgg cctggccgcc gcgatgacgt ggggcaagca gatcaagcgg atcgcccata
     1201 tccatcccga ctactcgtac ggccgcaacg ccttcgacca cttcaacatc gtgatgaaga
     1261 agatggtgcc cggcgcccag accgtctcgg agggctggcc gaagctcggg accaccgact
     1321 tcacctcgca catcacgaag gccatctcgt ccaagccgga tctgctggtc tcctcggtgt
     1381 ggggcggcga ctacgtggcg ctctacaagc aggcgctgcg ctacgacatg ttcaagaaga
     1441 tgaagttcgc gtccaccatc gccttcgggg tggccccgca cgccatcggc aaggatcatc
     1501 cggaaggcgc gatcgccggc gtgcactcca actactactt caactacccg ccgaacaatc
     1561 gctggccgct caacaccgag ttcgtgaaga agtactacga ccggtggaag gagtacccga
     1621 acttccagtc cgagggcgcc tactccaccc tctacatgct gaagaccgcg gtggagaagg
     1681 ccaacaagct cgcgggcggc tggccggacg acgacgcgat catcagccag ctcgagggcc
     1741 tgatgcatcc cggccccgcc ggctacgtct acatccgccc ggacacgcac cagggctaca
     1801 aggacgcgat gaccgggttc agcatcaact cgcccgacta tccgttccag atcctggatc
     1861 cgagccgcat catcacgatc ccgatccgca acatcacggc gcctcccggc tggcccaagg
     1921 gggagccgac ctcgacgtat acctggatcg acaagacctg gccgcagatg aaggcctgag
     1981 tcgggcggcc gaagaggccc tctcccccgc gagggggaga gggccggatg ctccggtgcg
     2041 atccttcacc gctctcgcct ccgctctcgc cgctctcgcg ctcctggccg ctccggcccg
     2101 cgccggccac gagctgccgt tctaccctgg ctactacccg caggagatcc ggctcgagac
     2161 cctgaccccc gccgcggcgg tgccgctact ccgtcgcagc acgctccacg cttacgtggg
     2221 ggccgatcct ttcgggggag gccgccttcc ggccaatgtg agcgcggtgg agtcgctcgc
     2281 gggctacgtc gtcgcgacgc ccggggccgc gacagcgggc gcggaccgca ccgcggcgcg
     2341 ctgcgcgggc gcgctcgccg tcgcgcgggc ggtcggcgcg gcccgcgtcg ccgggttcgt
     2401 ggcgcatccg tacgcggtca cgccgtacca cggcgactat ctcgagcact tcgacctggc
     2461 cgaggcctcg cgccgcgcgg tggagggccc ggccgcgggc gtgcccccgc gtctgctccc
     2521 ccgcggcccc ctcgcggagc gcctcgcgcc cgcgtcgaag ggcgggtccg cggccaaggg
     2581 cgacgcgatg gacgtcgagg agatcgacct ccagagcctg ctcgccccgt acatcgtcaa
     2641 cgcgggcggc tccttcggcc cgccctggct caaggagggg tggttccacg cctacctgct
     2701 gcaggcctcc acgatgaccg atgcgacggc ccgccaggcg gccgatcagc tcttccaccg
     2761 cctcgtcacc ggcgcctacg agggtcccgc cgagcgcgcc gacct
//
LOCUS       DATMCV010000463         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_891294, whole genome shotgun sequence.
ACCESSION   DATMCV010000463 DATMCV010000000
VERSION     DATMCV010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891294"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1334)
                     /locus_tag="VNU03_10670"
     CDS             complement(108..1334)
                     /locus_tag="VNU03_10670"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP74971.1"
                     /translation="MDLIIRNARLRHRDDLADVGIAGGRIATIAPRIPGGATEELDAA
                     GRLTTPSFVEPHIHLDKALTAERARENPTNVFEESIAIMREVKRAYTVEDVADRATRV
                     IHGLVGYGVTLIRSYVDVDTIVGLRALDGVLAARERCRHLARIELIAFPQEGIWCDPG
                     TDELMRSAMERGADVVGGMPYAEMIERDSERHIDFAFELARRFDRDVQMHVDETDDPG
                     ARTLHAYAVKAIEAGWHGRVTADHVTALAAYDDSYAAKVIALVARARMSVVTLPTKLM
                     RGGVRDKEPRRRGLTRVRELLAAGVNVAYGQDVIQDGFLPVFGTGDPLQVGFLLAFGA
                     QFSSRASIETLYDMATVNGARIMRRPDYGLEVGAPADLVVVEASSVQEAYRTQPPRLA
                     VIQDGRVVARNGRVIV"
     gene            complement(1352..2356)
                     /locus_tag="VNU03_10675"
     CDS             complement(1352..2356)
                     /locus_tag="VNU03_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP74972.1"
                     /translation="MLLVLRRLPLLVALLAAISAAVFALTQYLPGDPAFVAAGGGDAT
                     PEMVAAARARLGLDRPLWVRYLYYMRNVAGGDFGNSIVNRRPVIDDLKKFWPASMELA
                     LAAIAISALVGIPLGVVSAAWSGRWPDRLITPASVFLGSMPVFWLGLMAVFVFYRQLD
                     LLPAGGRLTVGLTPPPVSTGMLVVDAALAGQWRVLADALRHLILPSLILSVISLAMIT
                     RVTRAALLDVLHEDYLRTARAKGLGEGRILLKHALRNASVPILTIVGLQLGQLIGGVV
                     VTETIFSWPGLGLYAVTAVENQDYPALLAFTLITTAVYVTINLAVDLLYPLLSPQTRH
                     "
     gene            complement(2358..>2458)
                     /locus_tag="VNU03_10680"
     CDS             complement(2358..>2458)
                     /locus_tag="VNU03_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535111.1"
                     /note="with GsiABD is involved in the transport of
                     glutathione into the cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HWP74973.1"
                     /translation="GLAIFITVLAFNVLGERVRDVLDPQSRAGARS"
BASE COUNT          377 a          821 c          941 g          319 t
ORIGIN      
        1 tcccctctgg ggagagggca gggtgagggg cggatttcct cgacgggccc ttgaacctcc
       61 ccctcacccc gaccctctcc ccaaagggga gagggagagg ttcgaagcta gacgatgacg
      121 cgaccgttgc gggccaccac gcgcccgtcc tgaatgacgg cgaggcgcgg gggctgggtg
      181 cgatacgcct cctgcaccga cgacgcctcg acgaccacga ggtcggcggg cgcgcccacc
      241 tcgaggccgt agtcggggcg ccgcatgatc cgcgcgccgt tcaccgtggc catgtcgtag
      301 agcgtctcga tgctggcccg cgaggagaac tgcgcgccga aggccagcag gaagcccacc
      361 tggagcggat cgccggtgcc gaacacgggg aggaacccgt cctggatcac gtcctgcccg
      421 taggcgacgt tgacccccgc ggcgagcagc tcgcgcacgc gcgtgaggcc ccggcggcgc
      481 ggctccttgt cgcggacgcc gccgcgcatg agcttggtgg gaagcgtcac gacgctcatc
      541 cgggcgcggg ccaccagcgc gatcaccttc gccgcgtagc tgtcgtcgta ggcggccagc
      601 gccgtcacgt ggtcggcggt cacgcgcccg tgccagcccg cctcgatggc cttgacggca
      661 taggcgtgca gcgtgcgcgc tccggggtcg tcggtctcgt cgacgtgcat ctgcacgtcg
      721 cgatcgaagc gacgggccag ctcgaaggcg aagtcgatgt ggcgctcgga gtcgcgctcg
      781 atcatctccg cgtacggcat gccgcccacc acgtcggcgc cgcgctccat cgcgctccgc
      841 atcagctcgt cggtgcccgg gtcgcaccag atgccctcct gggggaaggc gatcagctcg
      901 atccgggcga gatgccggca gcgctcccgg gcggccagga ccccgtcgag cgcccggagc
      961 ccgacgatgg tgtcgacgtc gacgtacgag cggatcagcg tcaccccgta ccccaccagc
     1021 ccgtggatca cccgcgtggc ccggtcggcc acgtcctcca cggtgtaggc gcgcttgacc
     1081 tcgcgcatga tggcgatgga ctcctcgaag acgttggtcg gattctcccg cgcgcgctcc
     1141 gcggtgaggg ccttgtcgag atggatgtgg ggctcgacga acgacggcgt ggtgaggcgg
     1201 ccggccgcgt cgagctcctc ggtggccccg ccggggatgc ggggggcgat cgtggcgatg
     1261 cggccgccgg cgatgccgac gtcggcgagg tcgtcgcggt ggcgaagccg cgcgttccgg
     1321 atgatcaggt ccatcgcgtc acctcggcga ctcagtggcg ggtctggggg ctcagcaggg
     1381 gatagaggag atcgacggcg aggttgatgg tgacgtacac cgccgtcgtg atgagcgtga
     1441 aggccaggag cgccgggtag tcctggttct ccaccgcggt caccgcgtag aggccgagcc
     1501 ccggccagga gaagatcgtc tcggtcacca ccacgccgcc gatgagctgg ccgagctgga
     1561 gcccgacgat ggtcaggatg gggacggacg cgttgcgcag ggcgtgcttg agcaggatgc
     1621 ggccctcgcc gaggcccttg gcgcgcgcgg tgcgcaggta gtcctcgtgg agcacgtcga
     1681 ggagggcggc gcgggtcacg cgcgtgatca tggccagcga gatcacgctg aggatcagcg
     1741 aggggaggat caggtggcgc agggcatcgg cgagcacgcg ccactggccc gcgagggccg
     1801 cgtcgacgac gagcatgccg gtgctcacgg gcggcggcgt caggccgacg gtgaggcgcc
     1861 cgccggcggg caggaggtcg agctgccggt agaagacgaa caccgccatc agcccgagcc
     1921 agaagaccgg catggagccg aggaacaccg aggcgggtgt gatgagccgg tccggccagc
     1981 ggccggacca tgccgccgag acgacgccga gcgggatgcc cacgagagcg ctgatcgcga
     2041 tggcggccag cgccagctcc atcgaggccg gccagaactt cttgaggtcg tcgatcacgg
     2101 gccgccggtt gacgatggag ttgccgaagt cgccgccggc gacgttgcgc atgtagtaga
     2161 ggtagcggac ccacagcggc cggtcgaggc cgaggcgtgc ccgggcggcg gcgaccatct
     2221 cgggggtcgc gtctccgccc ccggcggcca cgaacgccgg gtcgccgggc aggtactggg
     2281 tgagcgcgaa caccgcggcg gagatggcgg cgagcagcgc caccaggagc ggcaggcgtc
     2341 ggagcacgag cagcacgtca ggacctcgcg cccgcgcggc tctgcggatc gaggacgtcg
     2401 cgcacccgct cgcccagcac gttgaaggcg agcacggtga tgaagatggc caggccgg
//
LOCUS       DATMCV010000464         2536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_22125, whole genome shotgun sequence.
ACCESSION   DATMCV010000464 DATMCV010000000
VERSION     DATMCV010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22125"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..>2536
                     /locus_tag="VNU03_10685"
     CDS             32..>2536
                     /locus_tag="VNU03_10685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP74974.1"
                     /translation="MKLDTTSRAIRRAQSVGSRPRLEVRGKFFFAGDEKVPLRGVTYG
                     PFAPDADGYRFPAPEVVERDLALISELGANCLRLFTPPPRWLLDMAAERNLWVMVGIP
                     WAEHVCFLDSEEITDVVRRTVREVVEACRSHPAVGAFLIGNEIPPDIVRWYGPQRIAG
                     FLRELAGMIKSIEPTALVGYANFPSTEYLETDFTDFLAFNVYLHSEPQFRRYLSRLHS
                     LAGDRPLVLTEFGIDSFREGEIVQAATLSWQVRTALEMGVAGTVVFSFTDEWYTGGCD
                     IANWAFGLVDRERRRKPAFQAVQRWYAAPGLPALAEYPKVSVVICAYNAEPTMHACLT
                     SLQNLKYPAFEVIVVDDGSTDRTGKIADGFEGIHVIHQKNKGLSAARNVGIAASLGEI
                     VAFTDSDCVVDPDWLHYLVATFLQTGLPAVGGPNLPPPEDSVVAACVAASPGGPLHVL
                     LDDVEAEHIPGCNMAFRREVLEEIGGFDPIYRSAGDDVDVCWRLQDRGYHIGFSPAAM
                     VWHFRRNTMKAYIGQQKGYGKAEALLYFRHPQRFNALGYSRWRGRIYGGISALFSLRR
                     PVIYGGVFGRGLFQTLYQPPSTVLSYLPFTLEWNLVGALLLAYGAVKGGWYWIGAAPV
                     ALSWACCVWAAMRARVDTLAGGLQGRLLISVLTYFGPLLRSLERYKWWVRGLTAAQPS
                     VAERPVQALPWSWRERAFSAAFWTETALEKETILHGMMEVLAARKYFVLVDQGWSEWD
                     LEVHGGIWSRGRIKVCAENHGGERRVLRVKCALRTSRLARGMLAGGVVAAGLGLQLGV
                     PALAAVGAAAGVVAGVAILRDSVALGRMLYDALQGVA"
BASE COUNT          328 a          905 c          889 g          414 t
ORIGIN      
        1 cgcccggtgg gcggcctagg aggcttggac cgtgaagctc gataccacgt cgcgcgccat
       61 ccggcgcgcg cagtcggtgg gatcccgccc ccggctggag gttcgaggga agttcttctt
      121 cgcaggcgac gagaaagtcc cgctccgggg ggtgacctat ggtcccttcg ccccggatgc
      181 tgatggctat agattcccgg cccccgaggt cgtcgagcgc gacctggccc tgatcagcga
      241 gctcggggcc aactgcctcc ggctcttcac cccacccccc cgctggctcc tggacatggc
      301 ggcggagcgg aacctctggg tgatggtggg catcccctgg gccgagcacg tctgcttcct
      361 cgattccgag gagatcaccg acgtggtccg gcggacggtg cgcgaggtgg tggaggcctg
      421 ccgcagccat cccgcggtgg gcgcgttcct gatcggcaac gagatcccgc ccgacatcgt
      481 ccgctggtac gggccccagc ggatcgccgg gttcctgcgg gagctggccg gcatgatcaa
      541 gagcatcgag ccgaccgccc tggtcggcta cgccaacttc ccctccaccg agtacctcga
      601 gaccgacttc accgacttcc tggccttcaa cgtctacctg cattccgagc cccagttccg
      661 ccggtacctg tcgcgcctgc acagcctggc cggcgaccgg ccgctggtcc tgaccgagtt
      721 cggcatcgac tcgttccgcg agggggagat cgtgcaggcc gcgaccctgt cgtggcaggt
      781 gcgcaccgcg ctcgagatgg gcgtggcggg gacggtggtg ttctcgttca ccgacgagtg
      841 gtacaccggc ggctgcgaca tcgcgaactg ggccttcggc ctggtcgacc gggagcggcg
      901 ccgcaagccc gccttccagg cggtgcagcg ctggtacgcg gcacccgggc tgcccgcgct
      961 ggccgagtat cccaaggtct cggtggtgat ctgcgcgtac aatgccgagc cgaccatgca
     1021 cgcctgcctg acctcgctcc agaacctcaa gtacccggcc ttcgaggtga tcgtggtgga
     1081 cgacggatcg accgaccgta ccggcaagat cgcggacggc ttcgagggca tccacgtcat
     1141 ccaccagaag aacaaggggc tgagcgccgc gcgcaacgtg ggcatcgcgg cctcgctcgg
     1201 cgagatcgtc gccttcaccg actccgactg cgtggtggac ccggactggc tccactacct
     1261 ggtcgccacg ttcctccaga ccggcctccc cgcggtgggc gggcccaacc tgccgccgcc
     1321 ggaagactcg gtggtggccg cgtgcgtcgc cgcgtccccc ggcgggccgc tccacgtgct
     1381 gctggacgac gtggaggccg agcacatccc gggctgcaac atggccttcc gccgcgaggt
     1441 gctggaggag atcggcggct tcgatccgat ctaccgctcc gcgggcgacg acgtggacgt
     1501 ctgctggcgg ctccaggacc gcggctacca catcggcttc agcccggcgg ccatggtctg
     1561 gcacttccgc cgcaacacca tgaaggccta catcggacag cagaagggct acggcaaggc
     1621 ggaggcgctg ctctacttcc gtcatcccca gcgcttcaac gcgctcggct actcgcgctg
     1681 gcgggggcgt atctacggcg gcatctccgc cctcttctcg ctgcgccgcc cggtgattta
     1741 cggcggcgtg ttcgggcgcg ggctcttcca gaccctctac cagcctccgt ccaccgtgct
     1801 ctcctacctg cccttcaccc tcgagtggaa cctggtcggg gccctcctcc tggcctacgg
     1861 ggccgtgaag ggcggctggt actggatcgg ggcggccccg gtcgcgctct cgtgggcctg
     1921 ctgcgtctgg gccgcgatgc gggcacgggt ggacaccctc gcgggaggcc tccaggggcg
     1981 gctgctcatc tcggtgctga cctacttcgg cccgctcttg cgctcgctcg agcgctacaa
     2041 gtggtgggtc cgcggtctca ccgcggcgca gccctcggtg gccgagcgtc cggtccaggc
     2101 cctgccctgg tcgtggcggg agcgcgcctt ctcggccgcg ttctggaccg agaccgcgct
     2161 cgagaaggag acgatcctcc acggcatgat ggaggtcctc gccgcccgca agtacttcgt
     2221 gctggtggac cagggctgga gcgagtggga cctcgaggtg cacggcggca tctggtcgcg
     2281 cgggcggatc aaggtgtgcg ccgagaacca cggcggcgag cggcgcgtcc tgcgcgtgaa
     2341 gtgcgcgctc cggacctcgc ggctcgcccg cggaatgctg gccggcgggg tggtcgcggc
     2401 ggggctcggc ctgcagctcg gcgtgcccgc cctggccgcg gtcggcgcgg ccgcgggagt
     2461 cgtggccggc gtcgcgattc tccgcgactc ggtggcactg ggccgcatgc tctacgacgc
     2521 gctccagggc gtggcc
//
LOCUS       DATMCV010000465         8103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_891346, whole genome shotgun sequence.
ACCESSION   DATMCV010000465 DATMCV010000000
VERSION     DATMCV010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891346"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..316
                     /locus_tag="VNU03_10690"
     CDS             68..316
                     /locus_tag="VNU03_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74975.1"
                     /translation="MDKQLVGVSFQVSEAGPTTWTLRTNIAHYRYGGMTEANHTCKRP
                     RPLMVGDTVSVQRVAEPSKDVIVQAPCGCKLLLYSLRT"
     gene            377..568
                     /locus_tag="VNU03_10695"
     CDS             377..568
                     /locus_tag="VNU03_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74976.1"
                     /translation="MSERLVRQRLRDNKGRAYCADCMAKDLGRNPAELKPAMEALVLR
                     ERFVSAPCGCGASGLIYRW"
     gene            complement(576..3326)
                     /locus_tag="VNU03_10700"
     CDS             complement(576..3326)
                     /locus_tag="VNU03_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP74977.1"
                     /translation="MPVYYPESMGDPTPSALGGEPAGPRAFVQASERIVGLALQSIGG
                     RYARVYRIDPDRSAPVGVASAVPADARSADAGPAVADIAFSLLGSTSDGARRTPDVLA
                     ERECQFDPATRELIKRTRIGALATAPIRVRGAVVGSLVIADRTGRRFADHELLLLSAL
                     ADQVALVVENDRLEAETTRQQYEAGELALVASLIGERLDPLSAGQRIAESVLGLLGVH
                     SAVIRLFQPDGALGAIALAGRAKEYAEIGDRIPAGLGLVGRAAVEGRPVWTSDIRVDG
                     RFEQSPTLRARNVAVGIVAGLAVPMHAAGVVIGVLSVGSGVPRTFTQREIDLLQRFAD
                     QAALTISNVRTQETLVKQAKRLQILHDIDLAIITETAPAAIAEAVLGRLRELLGVPRA
                     IVNLFDWGAEEVEWLAAVGRHRIHRGPAVRYPLRFAGDLDALRRGEPQVVDVRTLPAS
                     PEADSLMASGVHLYMVVPMIAAGELIGSVSFGGDQETFPDEQISVAREVATQLAIALG
                     QARLVERLKASYAELEQAQAQLAQSQKMEAIGQLAGGIAHDFNNLLTVIGGRSSLLLM
                     RLAPDDPARKDIDLIQTTAQRAAGLTRQLLAFSRKQLLEPKPINLKTLVAGVTPILTR
                     LIGEHIEIVIVAADGAAQVLADPGQMEQVIVNLVVNARDAMSDGGTLTIETSDRVVPE
                     GGLRAHDRPVPPGPYVTLSIRDTGSGMDAATLARVFEPFFTTKEPGRGTGLGLSTVHG
                     IVHQSGGYLAVESVVGRGTAFTIYLPRLLDPISASGAGQVLVPDLVGGSETVLLVEDD
                     EELRKLTSEILQASGYTVLEAGDPLEALTLCDRRDSKPIDLLLTDMVMPAMRGPELAA
                     TLSETRPGLKVLFMSGYADESVTSPQGEPGRAFLQKPFTPHDLTRAVREALASRGAGP
                     AR"
     gene            complement(3442..3645)
                     /locus_tag="VNU03_10705"
     CDS             complement(3442..3645)
                     /locus_tag="VNU03_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74978.1"
                     /translation="MPINDRPLSPSVPGRLPCATRVEVDAGIDIVSAADVRAWVTHLR
                     SHGWTDSELDLVWITRARQGVVR"
     gene            3916..4185
                     /locus_tag="VNU03_10710"
     CDS             3916..4185
                     /locus_tag="VNU03_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74979.1"
                     /translation="MPAVILSVGDSVRRVVDGLLRGACWGKFLCSRCLVKLTRDHLDR
                     RHPTREVTLAIADIFRAPGRMTHLARSTCVACAESTMPCLGVPVA"
     gene            4182..4457
                     /locus_tag="VNU03_10715"
     CDS             4182..4457
                     /locus_tag="VNU03_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74980.1"
                     /translation="MSLPYTTEGFIRRTVGGVLDGDYRGRGVCSACLVGMALERLHRG
                     WRRSEVERAMGKVFEAPAPLGGMPTGPCGRCRRPTPCLRGGPPARSR"
     gene            4473..4763
                     /locus_tag="VNU03_10720"
     CDS             4473..4763
                     /locus_tag="VNU03_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74981.1"
                     /translation="MIGEELARCHVGAMLRDQPGRYYCARCLAALLDGAVSTKREARR
                     AIAVLFRRPIGLTATLRGGRQPCRRCGLVGGARLGAAPSTWDTFLDRTVMAR"
     gene            4928..5128
                     /locus_tag="VNU03_10725"
     CDS             4928..5128
                     /locus_tag="VNU03_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWP74982.1"
                     /translation="MAQGTVKWFNDAKGYGFITQEAGEDVFVHFNAIQGQGFKSLAEG
                     DKVEFEVTKGPKGLQAANVRKV"
     gene            5305..5631
                     /locus_tag="VNU03_10730"
     CDS             5305..5631
                     /locus_tag="VNU03_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74983.1"
                     /translation="MFEDKARVLLILPQQVLDRARVFAGESITRLKAPVSLQMVLRAL
                     IDEGLKRDRDRAIVANIEGQMQAVRAIRRRGRRPAAGHRTPPSVRAAGRRGPKGRVGV
                     ARHGGK"
     gene            5642..6109
                     /locus_tag="VNU03_10735"
     CDS             5642..6109
                     /locus_tag="VNU03_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74984.1"
                     /translation="MTRRHFMGDRTYKGVTYTLQLEAVEDGVFKAHARLLPSGPEVSF
                     VDEASDRALNQAEAEIKQIIERREASAVVRDRLPERKYRDVTYTIIVRTEATRVVTEV
                     YFIRTNAVQRRPGPGTFFALFQGKTRERAIAQAERTFKAKIDEEHAGARGRKV"
     gene            6130..6417
                     /locus_tag="VNU03_10740"
     CDS             6130..6417
                     /locus_tag="VNU03_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74985.1"
                     /translation="MREPGLGLDVWWGEREYRACGASLECRGRYIVGKLFDLDTRMLQ
                     TAWLAAFGRRLLGPPEIQAHEITRSQWQLILSHMGARADEIVDVQTRMGRT"
     gene            6529..6918
                     /locus_tag="VNU03_10745"
     CDS             6529..6918
                     /locus_tag="VNU03_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HWP74986.1"
                     /translation="MANSAGYHEAEGKLRPETLDNHRALTSMQEELEAADWYDQRVDA
                     ATDPALKEILAHNRDEEKEHFAMLLEWYRRRDAKMDEHLKEYLFSTGSLIAREQAVTG
                     EGGAAAGATAGGGGSLGIGSLKGGQGL"
     gene            6915..7745
                     /locus_tag="VNU03_10750"
     CDS             6915..7745
                     /locus_tag="VNU03_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HWP74987.1"
                     /translation="MNNLKRELAPISTAAWQEIDAEATRVLKLKLAGRKLVDFEGPLG
                     PTEAAVNTGRRERLGAAPVADVEASRRQVLPLVELRAYFDLSREEMDAVERGAKDPEL
                     QPLIDAATRIAYAEDTAIFHGYAAGGIKGIDQVTAYPILPIPDDYQDYPRSIAEATRL
                     LRLAGVDGPYAIALGPRSYTGLTQAVGDGGYPVLNVVRKLVDGPIVWAPAISGAVVMS
                     QRGGDFELTVGRDMSIAYQSHNDTSVRLYLVESMTFRVLTPEAAVALAHKDTKASKKG
                     "
     gene            7786..>8103
                     /locus_tag="VNU03_10755"
     CDS             7786..>8103
                     /locus_tag="VNU03_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74988.1"
                     /translation="MKLVALGLTVVVALGAHAPSASAQSSPTGAARVGDLALSRAIGP
                     PELPPQYWDALRAGLRERGYVEGRNVVFERREARDRPDLLPAAAAELARLNVDVILAR
                     GAQA"
BASE COUNT         1240 a         2737 c         2968 g         1158 t
ORIGIN      
        1 cctggagagc gtaggctaat aacagcgctc acatgagcga gagatcgagg gaggcgttcg
       61 accgagaatg gacaagcagc tggtgggggt gtccttccag gtgtccgaag cgggtccgac
      121 cacctggaca ctcaggacca acatcgctca ctatcggtac ggcggcatga ccgaggcgaa
      181 ccacacgtgc aagcgtcccc ggccgctcat ggtcggcgac accgtctcgg tccagcgtgt
      241 cgcggagccg agcaaggacg tgatcgtcca ggcgccgtgt ggctgcaagc tgcttctgta
      301 ctccctgcgc acctgacgtg accgttagtg ggtcctggct tgccgtgacc cgggaggtgg
      361 aaccatggcc tacagcatga gcgagcgact tgtgcgacag cgactgcggg acaacaaggg
      421 gcgggcctac tgcgccgatt gcatggcgaa ggacctgggg cgaaatccgg ccgagctcaa
      481 gccggcgatg gaggccctgg tgctacggga gcgcttcgta tccgcgccct gcggctgcgg
      541 cgcgagcggc ctgatctacc gctggtaaga ctgcactacc gcgccggtcc ggcgcccctc
      601 gaggcgaggg cctcgcgaac cgctcgggtc aggtcgtggg gcgtgaacgg cttctgcagg
      661 aaggcccggc cgggctcgcc ctgaggtgag gtgaccgact cgtccgcgta gcccgacatg
      721 aagagcacct tcaggccggg gcgggtctcc gacagcgtgg cggccagctc cggtccccgc
      781 atcgccggca tcaccatgtc ggtcaggagg agatcgatcg gcttgctgtc ccgccggtcg
      841 cacagggtga gagcctcgag gggatccccc gcttccagga ccgtgtagcc ggaggcctgg
      901 aggatctccg aggtcagctt gcgtagctcc tcatcgtcct cgacgaggag caccgtctcc
      961 gagcctccca cgagatccgg cacgaggacc tgcccggcgc cggacgcgct gatcgggtcc
     1021 aggagacgcg gcaggtagat cgtgaaggcg gtgccccggc ccaccacgct ctcgaccgcc
     1081 aggtagccgc cgctctggtg cacgatgccg tggacggtcg agaggccgag gccggtcccg
     1141 cggccgggct ccttcgtggt gaagaagggc tcgaagactc gcgcgagcgt ggccgcgtcc
     1201 attccgctcc cggtgtcgcg gatgctcagg gtgacgtacg ggccgggcgg cacggggcga
     1261 tcgtgggcgc ggaggccgcc ctcgggaacc acgcgatccg acgtctcgat ggtgagcgtt
     1321 ccgccgtccg acatcgcgtc ccgggcgttg accaccaggt tcacgatgac ctgctccatc
     1381 tggccgggat cggccagcac ctgggccgcg ccgtcggccg ccacgatcac gatctcgatg
     1441 tgctccccga tgaggcgcgt caggatgggg gtgacgccgg ccaccagggt cttgaggttg
     1501 atgggcttcg gctcgaggag ctgcttgcgg ctgaacgcga ggagctggcg ggtcagcccg
     1561 gcggcccgct gcgcggtcgt ctggatcagg tcgatgtcct tgcgcgcggg atcgtccggg
     1621 gcgagcctca tcaggaggag gctgctgcgg cctccgatca cggtcagcag gttgttgaag
     1681 tcgtgggcga tgccgcccgc gagctggccg atcgcctcca tcttctggga ctgggcgagc
     1741 tgggcctggg cctgctccag ctccgcgtac gaggccttca gccgctcgac gagccgggcc
     1801 tggccgaggg cgatcgcgag ctgggtcgcc acctcgcgcg cgacgctgat ctgctcgtcg
     1861 gggaaggtct cctggtcgcc gccgaagctc accgagccga tgagctcgcc cgccgcgatc
     1921 atcggcacca ccatgtacag gtgaacgccc gaggccatca gggagtcggc ctcggggctc
     1981 gcggggagcg tgcgcacgtc gacgacctgg ggctccccgc gtcgcagggc gtccaggtcg
     2041 cccgcgaatc gcaggggata ccggaccgcg gggccgcggt ggatacgatg gcgcccgacc
     2101 gcggccagcc actccacctc ctccgccccc cagtcgaaga ggttcacgat cgcgcggggg
     2161 acgccgagga gctcgcggag gcggccgagc accgcctccg cgatcgcggc cggcgcggtc
     2221 tcggtgatga tggcgagatc gatgtcgtgc aggatctgga gacgcttcgc ctgcttgacg
     2281 agcgtctcct gggtgcggac attgctgatg gtgagcgcgg cctgatcggc gaagcgctgc
     2341 aggaggtcga tctcccgctg ggtgaacgtc cgcggcacgc cgctccccac cgacagcacg
     2401 ccgatcacca ctccggccgc gtgcatgggg accgcgagcc cggccacgat cccgaccgcc
     2461 acgttccggg cgcgcagggt cgggctctgc tcgaagcggc cgtcaacgcg gatgtcgctg
     2521 gtccagaccg ggcgtccctc gaccgcggcg cgcccgacga gaccgagccc ggcggggatg
     2581 cggtccccga tctccgcgta ctccttcgcg cgaccggcca gggcgatggc cccgagggcg
     2641 ccgtcgggct ggaagagccg gatcaccgcg ctgtggaccc ccaggaggcc gagcacgctc
     2701 tccgcgattc gctgccccgc cgagagcggg tcgaggcgct cgccgatcag gctggccacc
     2761 agggccagct cgccggcctc gtactgctgc cgggtggtct cggcctcgag gcggtcgttc
     2821 tcgacgacga gcgccacctg gtcggcgaga gcggagagga ggaggagctc gtgatccgcg
     2881 aagcggcggc cggtccggtc ggcgatgacg aggcttccga ccaccgcgcc gcggacacgg
     2941 atcggcgcgg tggcgagcgc gccgatccgc gtccgcttga tcagctcgcg ggtggccggg
     3001 tcgaactggc actcgcgctc ggcgaggacg tccggcgtgc gacgggctcc gtcggatgtc
     3061 gaccccagga ggctgaacgc gatgtccgcc acggcgggtc cggcgtcggc cgatctcgcg
     3121 tcggccggga ccgcggacgc cacgcctacg ggcgcgctcc ggtccgggtc gatccggtac
     3181 acgcgggcgt agcgaccacc gatcgactgg agagccaggc ccacgatcct ctccgaggcc
     3241 tggacgaagg cgcgggggcc tgcgggctca ccaccgagcg cagacggggt tggatccccc
     3301 atggattcag ggtagtacac gggcatttca taaggcaatt tactggtggg gggcgctcac
     3361 ccgcattcgt gcctcatccc acgcccgagg gcggtaagat gttccccagc ccggcgcgcc
     3421 gacgacgcgc gcgccgggtg cctagcgaac caccccttgc cgcgcgcggg tgatccacac
     3481 caggtccagc tcgctatcgg tccagccgtg cgagcggagg tgggtcaccc aggcccgcac
     3541 gtcggcggcc gagacgatgt cgatgccggc gtcgacctcg acacgggtgg cgcacggaag
     3601 acggccgggc acggacgggg agagcgggcg gtcgttgatg ggcatctcag ggacccctcg
     3661 atggagaccg gaatcgactg cggggcgagg accgagcaca cggcgggggc gccagtacct
     3721 gccgatctcc tcaggggaga ggggccgact atagcgcaaa cgcggccgcg gcacgcggcc
     3781 aatctcggtc gcgccgccga gggggattcg gcttgaccgc gtgacgcacc cgatataaaa
     3841 tcgatacaac ttatatatca tataagtcta gacgctctcg ctcgggcgcc ccggggtggc
     3901 gcggaggctt cgcagatgcc ggcagtgatt ctgagcgtcg gcgactccgt ccggcgggtg
     3961 gtcgacggtc ttctccgcgg ggcctgctgg ggcaagttcc tctgctcgcg ctgcctggtc
     4021 aagctgaccc gggaccacct ggaccgacgc cacccgacgc gcgaggtcac gctggccatc
     4081 gccgacatct tccgggcccc cggccgcatg acccacctgg cgcgatcgac ctgcgtggcg
     4141 tgcgcggaga gcacgatgcc ctgcctcggg gtgccggtcg cgtgagcctc ccgtacacca
     4201 ccgagggctt catccggcga acggtcggcg gggtgctcga cggcgactat cgcggacggg
     4261 gagtctgctc ggcctgcctg gtggggatgg ccctcgagcg gctgcaccgc ggctggagac
     4321 ggtccgaggt cgagcgggcc atgggcaagg tcttcgaggc ccccgcaccc ctcgggggca
     4381 tgccgacggg gccctgcggg cgctgccggc gcccgacgcc gtgcctgcgc ggcggcccgc
     4441 ccgcgcgtag tcgctgaccg ctcggtgacg tcatgatcgg cgaggagctc gcgcggtgcc
     4501 atgtgggggc gatgctccgc gaccagccag ggcgctacta ctgtgcccgc tgcctcgccg
     4561 ccctcctcga cggcgcggtg tcgacgaagc gagaggcgcg ccgggcgatc gcggtgctct
     4621 tccggcggcc catcgggctg accgccaccc tgcgaggggg ccggcagccc tgccgtcgct
     4681 gcggactggt cggcggcgcg cggctggggg cggcgccgtc cacctgggat acattccttg
     4741 acaggaccgt gatggctcgg tagcctgtcc atcggtgcaa gggcttgagc acgaggttcg
     4801 agcgtaacgc cgggcggacg tcaggtcgct cggcgttttt gtttgtcctc cacttcagga
     4861 gggcatcccc gccatcgggg cggggtttga cggaggcgta aagcctcgcc agaaagaggg
     4921 cgcgtcgatg gcacagggta cggtcaagtg gttcaatgat gcgaagggct acggtttcat
     4981 cacccaggag gctggcgagg acgtgttcgt gcacttcaac gccatccagg gccagggctt
     5041 caagagcctg gcggagggcg acaaggtcga gttcgaggtg accaagggtc cgaaggggct
     5101 ccaggccgcg aacgtccgca aggtctgaac ccgccccacc gcatcacccg ccggccggat
     5161 ccctccgggg atccggccgg ttcatctccg ccccccgcgg accgacaatc atgatataag
     5221 ttacataaca agatctaaat atatgtcgga cgccccttac acgaacgccc tttcatgcac
     5281 gaacgccctt tcatggaggt cgcaatgttc gaggacaagg cccgggtcct gctcattctc
     5341 ccccagcaag tcctggatcg ggcgcgcgtg ttcgcgggcg agtcgatcac gcgattgaag
     5401 gctccggtga gcctccagat ggtgctgcgc gcgctcatcg acgaggggct gaagcgggac
     5461 cgcgatcggg ccatcgtcgc caacatcgag ggccagatgc aggcggtccg ggcgatccgg
     5521 aggcgtggcc gacgtcccgc ggcgggtcac cgcacccccc caagcgtccg cgcggcgggg
     5581 cggcgaggcc cgaaggggcg ggtgggggtg gcgcgccatg gaggcaagta ggcggtacgt
     5641 gatgacccga cggcacttca tgggtgatcg cacctacaag ggcgtgacct acaccctgca
     5701 gctcgaagcg gtcgaggacg gcgtgttcaa ggcgcacgcg agactcctgc cctcgggccc
     5761 cgaggtgtcc ttcgtggacg aggcctccga tcgcgcgctg aatcaggccg aggccgagat
     5821 caagcagatc atcgagcgcc gggaggcgag cgcggtggtg cgcgaccgcc tgccggagcg
     5881 gaagtaccgc gacgtcacct acacgatcat cgtccgcacc gaggcgacgc gggtggtgac
     5941 cgaggtgtac ttcatccgca ccaatgcggt ccagcgccgg ccgggtcccg gcaccttctt
     6001 cgcgctcttc cagggaaaga ccagggagcg ggccatcgcc caggccgagc ggaccttcaa
     6061 ggccaagatc gacgaggagc acgcgggcgc ccggggccgg aaggtctagg cttgtcgagc
     6121 gtggcaggaa tgcgggagcc gggactcggt ctggacgtct ggtggggcga gcgcgagtac
     6181 cgggcgtgcg gcgcgtcgct cgagtgtcga ggccggtaca tcgtcggcaa gctgttcgat
     6241 ctcgacaccc ggatgctcca gaccgcgtgg ctcgccgcct tcggacgtcg gctgctcggc
     6301 ccccccgaga tccaggccca cgagatcacg cggtcgcagt ggcagctaat cctgtcccac
     6361 atgggtgccc gcgcggacga gatcgtcgac gtccagacgc ggatgggtcg aacctaggcc
     6421 ccgcgcgccc ctttccggga accgcgtgcg agcgaccacc gtctggagta ttgtgacgag
     6481 gttctcccac gtgcgagcac aacccaatga cctctcggag gggggctgat ggcgaactcg
     6541 gcgggatatc acgaagcgga gggcaagctc aggccggaga cgctggacaa tcaccgcgcg
     6601 ctgacctcga tgcaggagga gctggaggcg gccgactggt acgaccagcg ggtcgacgcc
     6661 gcgaccgatc cggccctgaa agagatcctg gcccacaatc gcgacgagga gaaggagcac
     6721 ttcgcgatgc tgctggagtg gtaccggcgc cgggacgcca agatggacga gcatctgaag
     6781 gagtacctgt tcagcaccgg ctccctcatc gcccgggagc aggcggtgac cggtgaaggc
     6841 ggcgccgccg cgggcgccac ggccggtggc ggcggctcgc tcggcatcgg cagcctgaag
     6901 ggagggcagg ggctatgaac aacctgaagc gagagctggc cccgatctcg acggcggcct
     6961 ggcaggagat cgacgccgag gccactcgcg tcctcaagct gaagctggcc ggccggaagc
     7021 tggtggactt cgaggggccg ctcgggccca ccgaggccgc ggtgaacacc ggacgccgcg
     7081 agcggctcgg ggcggcgccg gtcgccgacg tggaggcctc gcgccggcag gtgctcccgc
     7141 tcgtcgagct gcgggcgtac ttcgatctgt cccgcgagga gatggacgcg gtcgagcggg
     7201 gcgcgaagga cccggagctc cagcccctga tcgacgcggc cacccgcatc gcctacgcgg
     7261 aggacaccgc gatcttccac ggctacgcgg ccgggggcat caaggggatc gaccaggtca
     7321 ccgcctatcc gatcctgccg attcccgacg actaccagga ctatccccgg agcatcgcgg
     7381 aggcgacgcg gctcctgcgc ctggccggcg tcgacggccc gtatgccatc gcgctgggac
     7441 cgcgctccta caccggcctc acccaggcgg tcggcgacgg tggctacccg gtcctgaacg
     7501 tggtgcggaa gctggtggac gggccgatcg tgtgggcccc cgccatcagc ggcgcggtgg
     7561 tgatgagcca gcgcgggggc gacttcgagc tgaccgttgg tcgggacatg tcgatcgcct
     7621 accagagcca caacgacacc tcggtccgcc tctacctggt cgagtccatg accttccggg
     7681 tgctgacgcc ggaggcggcg gtggcgctcg cccacaagga caccaaggcg tcgaagaagg
     7741 gctgatcccg cttcgcgccg gggacgacgg cgagcggccg cggggatgaa gctcgtcgcg
     7801 ctcggcctca ccgtcgtcgt cgccctcggc gcccatgccc cctcggcgag cgcgcagtcg
     7861 tccccgaccg gggccgcgcg cgtgggagat ctggcgctct cgcgggccat cggcccgccc
     7921 gagctgccgc cgcagtactg ggacgcgctc cgggccggtc tgcgcgagcg cggctacgtc
     7981 gagggccgga acgtggtctt cgagcgccgc gaggcccggg accgcccgga cctgctcccg
     8041 gcggcggcgg ccgagctggc tcgactgaac gtcgacgtca tcctcgcgcg gggagcgcag
     8101 gcg
//
LOCUS       DATMCV010000466         3053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_223423, whole genome shotgun sequence.
ACCESSION   DATMCV010000466 DATMCV010000000
VERSION     DATMCV010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223423"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..524
                     /locus_tag="VNU03_10760"
     CDS             156..524
                     /locus_tag="VNU03_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74989.1"
                     /translation="MSRLVPTIVLIAILVGTAGCASLPGAAGAPIADAGALAGKWAGI
                     VTPGDEPFYLTINPGGTLTAAWGPNMAWGTVTVRNGQATFEMQPGPYEGSIRLYDDGG
                     TRQIVLDDFWASFNARAVPQ"
     gene            521..862
                     /locus_tag="VNU03_10765"
     CDS             521..862
                     /locus_tag="VNU03_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74990.1"
                     /translation="MRIRLGRWALGIAAALALAGCASVGAGRGAGGAAPGALDLELGY
                     GGAPRVDLPIAPPDTNADAAQALQAIESRRKAEEALGRESRPDPTRRPDLDHDVTQGI
                     QSRGLDRAIGH"
     gene            892..1227
                     /locus_tag="VNU03_10770"
     CDS             892..1227
                     /locus_tag="VNU03_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74991.1"
                     /translation="MRTIRALSIVVTLGLAVLTTAGPAAAYVIEALTSIPADQATDKE
                     TLERAIRVAVDDVATHAVAFTPTVVSLREAKLIGDRIYLFVLLADEAGEAEIHVLETG
                     TARSSAGTL"
     gene            1247..1654
                     /locus_tag="VNU03_10775"
     CDS             1247..1654
                     /locus_tag="VNU03_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 family protein"
                     /protein_id="HWP74992.1"
                     /translation="MFDTKIAIVVREDLAVWQKLNVTAFLTSGIVGATEGLLGEAYED
                     AAGNTYNPLAIQPMIVLSADADTVRAIYRRAMEQKIRLSLYIEDMFRTGHDAANRETV
                     KRCRPEEMNVVGLALRDDKKVVDKITKGARMHP"
     gene            complement(1667..2017)
                     /locus_tag="VNU03_10780"
     CDS             complement(1667..2017)
                     /locus_tag="VNU03_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74993.1"
                     /translation="MSRAPRPGWFCGALLAALDASEGRRRRRKRDTTPDAIGMALKRE
                     LLEAATADDPDPDDFEAWLLDRCLRGADPTARGARTAMARDILAEWRLAAASPAFRDW
                     LEAGAPSEDRDAAG"
     gene            2212..2643
                     /locus_tag="VNU03_10785"
     CDS             2212..2643
                     /locus_tag="VNU03_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74994.1"
                     /translation="MNGKVWRVVAIAGLSGLMIGVPGAGQAADGDDERSVAALFDESP
                     VRPDQLFRVEWVASPARSGRSRLEGSVHNDFGRTALNVQLRVSEIDAAGETVATVIGP
                     TLERVPGQGRVRFDVQVPDHRRSYRVAVASFSFDFADPAAR"
     gene            2655..>3053
                     /locus_tag="VNU03_10790"
     CDS             2655..>3053
                     /locus_tag="VNU03_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74995.1"
                     /translation="MNRMLAVLLSALLVAPSLAACTTALPPIPSIPSEMGPVAADPPE
                     DAWPRELVGSDETFSIFQPQYDRWEQGRLDGRAAVAVESQASPEPRYGVMWFTARTQV
                     DKDSRMVTLEDLTVSRVDFPTATRDGAGYLA"
BASE COUNT          436 a         1070 c         1130 g          417 t
ORIGIN      
        1 acgggcgtgc tcgccctgcg cgtgaccggg cggccgcgcc cgtcgcccga ctgcgcgagc
       61 agcgtggtca tcgtccccgg cccgcatggc cagccggtgg agtgcgtctg catcggcgga
      121 acggtctcca cctgcttcga cccggggccg tagcgatgtc ccgcctcgtt cccaccatcg
      181 ttctgatcgc gatcctcgtc ggcacggccg gatgtgcgtc cctgcccggg gcggccggcg
      241 cgccgatcgc cgacgccggc gcgctcgccg ggaagtgggc gggcatcgtg accccgggcg
      301 acgagccgtt ctacctgacg atcaacccgg gtgggacgct gaccgcggcg tggggcccga
      361 acatggcctg gggaacggtg acggtccgca acgggcaggc gaccttcgag atgcagccgg
      421 gtccctacga gggctcgatc cggctctacg acgacggcgg cacgcgccag atcgttctgg
      481 acgacttctg ggcgtcgttc aacgcgcggg cggtgcccca gtgagaatca ggctcgggcg
      541 gtgggcgctc ggtatcgcgg ccgcgctggc gctcgccggg tgcgcgtcgg tcggggccgg
      601 ccgcggggcg ggcggcgccg cgcccggcgc cctcgacctc gagctgggct atggcggcgc
      661 gccgcgggtc gatctgccga tcgccccgcc cgacacgaac gcggatgccg cgcaggcgct
      721 ccaggccatc gagtcgcgcc gcaaggcgga ggaggcgctc ggccgggaga gccggccgga
      781 tcccacgcgc cgccccgacc tcgatcacga cgtcacccag ggtatccagt cgcgcggcct
      841 ggaccgggcg atcggccact gagccgatcg cggaatgcga ggaggagcga catgcggacc
      901 attcgtgcac tctcgatcgt ggtaaccctc gggctggccg tcctgaccac ggccggcccc
      961 gccgccgcgt acgtcatcga ggcgctgacc tcgatcccgg ccgaccaggc gacggacaag
     1021 gagaccctgg agcgggcgat ccgcgtggcg gtcgacgacg tcgcgaccca cgcggtcgcc
     1081 ttcacgccga cggtggtgtc gctccgcgag gccaagctga tcggcgatcg gatctacctg
     1141 ttcgtcctcc tcgcggacga ggcgggtgag gccgagatcc acgtgctgga gaccgggacg
     1201 gcgcggtcga gcgcgggcac gctctgactc tgctagtcta gggcgcatgt tcgatacgaa
     1261 gatcgcgatc gtcgtgcgcg aggacctggc ggtgtggcag aagctgaacg tgaccgcgtt
     1321 cctgaccagc gggatcgtcg gcgcgaccga gggcctgctc ggagaggcgt acgaggacgc
     1381 ggccggcaac acctacaatc cgctcgcgat ccagcccatg atcgtgctga gcgccgacgc
     1441 cgacacggtg cgggcgatct atcgccgcgc gatggagcaa aagatccggc tctccctcta
     1501 catcgaggac atgttccgga ccggccacga cgcggccaac cgggagacgg tgaagcgctg
     1561 ccgtcccgag gagatgaacg tggtcgggct cgccctgcgc gacgacaaga aggtggtcga
     1621 caagatcacc aagggcgccc gcatgcatcc ctagtcggtc gcacccctag ccggccgcgt
     1681 cccggtcctc ggacggcgcg ccggcctcga gccagtcgcg gaatgcgggg gaggcggcgg
     1741 cgagccgcca ctcggccagg atatcgcgcg ccatcgcggt ccgggccccc cgggcggtcg
     1801 ggtcggcacc ccggagacag cgatcgagga gccaggcctc gaagtcgtcc ggatcggggt
     1861 cgtccgccgt cgccgcctcg agcagctcgc gcttgagcgc catgccgatg gcgtccggag
     1921 tggtgtcgcg cttccgtcgg cggcgccgtc cctcggaggc gtcgagggcc gcgagcaggg
     1981 ccccgcagaa ccatcccggt ctcggcgctc ggctcatgac gttctcatcc cacgcgggac
     2041 tttagacgag ccgcggctcg cgcccggatc ggagcccgag ccctttccgc aagatccgac
     2101 aaagacgtcg cgcactcttg cgcgaactcc gccgggggga cggcggcatc ccgggcgagc
     2161 ccgtcacaag gacagcaacg agccatgacg ggcgatgacg ggaggtcgca gatgaacggc
     2221 aaggtgtggc gagtggtggc gatcgcaggg ctgtccggac tcatgatcgg ggtgcccggg
     2281 gccggccagg ccgcggacgg cgacgacgaa cggtcggtgg ccgcgctgtt cgacgaaagt
     2341 ccggtgcgcc ccgaccagct cttccgggtg gagtgggtgg cgagcccggc gcgctccggc
     2401 cgctcgcggc tcgaaggctc ggtgcacaac gacttcgggc gcaccgccct gaacgtgcag
     2461 ctccgggtga gcgagatcga cgcggcgggc gagacggtcg ccaccgtgat cgggcccacg
     2521 ctcgagcggg tgccgggaca gggtcgggtg cgcttcgatg tccaggtgcc cgatcaccgc
     2581 cgctcgtacc gggtggcggt ggcgtcgttc tcgttcgact tcgccgaccc cgcggcgaga
     2641 taggggaggc gacgatgaac cggatgctcg ccgtccttct ctccgcgctc ctggtggcgc
     2701 ccagtctcgc cgcatgcacg accgcgctgc cgccgatccc gtcgatcccg tccgagatgg
     2761 gtccggtcgc ggccgacccg cccgaggacg cgtggccacg cgagctcgtc ggctcagacg
     2821 agacgttctc gatcttccag ccccagtacg atcgatggga gcaaggccgc ctggacggcc
     2881 gggccgcggt ggcggtggag agccaggcgt cgccggagcc tcggtacggg gtgatgtggt
     2941 tcaccgcccg gacccaggta gacaaggact cccggatggt cacgctcgag gacctgaccg
     3001 tctcccgggt cgacttcccg accgcgacgc gtgacggcgc gggctacctg gcc
//
LOCUS       DATMCV010000467         2271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_22179, whole genome shotgun sequence.
ACCESSION   DATMCV010000467 DATMCV010000000
VERSION     DATMCV010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22179"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VNU03_10795"
     CDS             complement(<1..449)
                     /locus_tag="VNU03_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP74996.1"
                     /translation="MATETYSLEQFVADLDRITGRESEPAAITAQVAPLLGRLVRNDA
                     AIPAPFRGRGPDGGRGRFMLHRAPRFNVTAVTWRPGDRAAAHNHDTWGVIGVVDNEIE
                     ETRYRVAEGPGGGAALTVQSVTRHGPGAISLLVPPDDEVHAMYNPTGR"
     gene            complement(449..1309)
                     /gene="sseA"
                     /locus_tag="VNU03_10800"
     CDS             complement(449..1309)
                     /gene="sseA"
                     /locus_tag="VNU03_10800"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HWP74997.1"
                     /translation="MSAALPDPLVSTEWLAAHLGEPDLKVVDATFYLPHLKRDAAAEF
                     EQAHVPGAVFFDVETIADHANPLPHMLPDAKAFADAMGALGIAAGDRVVAYGGRGLIA
                     SARAWWTFRVFGHDRVAVLDGGSARWKQEGRPLEAGRASPAARRFVATYRPELVADLD
                     RMRAILERRDAQVVDARSRGRFAATEPEIRPGLRGGHIPGSRSLPYTELFRADDDAML
                     PAEDVGAAFARAGLDPARPVVASCGSGVSAAVLALGLYRLGRRDAAVYDGSWTEWGGR
                     ADTPVATGEA"
     gene            complement(1306..>2271)
                     /locus_tag="VNU03_10805"
     CDS             complement(1306..>2271)
                     /locus_tag="VNU03_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWP74998.1"
                     /translation="NGTMGWQLDGLELERGASRWPPAPSAASRARAREVAARLAAEDG
                     TPLVSPDAVAERWSHRDRESVSMLDVRTREEYEAGHVPGSIWAPGGQAVQATDEYVAV
                     RAGWLVLICDGFGRSIMTAGWLKRMGFPHVAVLEGGVPAWERSGRPLETGQPAPAPFG
                     YEAAQRVVERVAPGPLGNDVVVSVDPSDVYQRGHLPGAIWIGRGRLELAIGRAAPDPT
                     RRVLLTCADGVQSTLAAATLRRMGYAAARVLDGGVAAWRAAGLPVETGLGSMQGEADD
                     VVQKPYERGRAAMEAYLRWEEALDAHGVSPHALLPERPAAPGSTR"
BASE COUNT          268 a          886 c          850 g          267 t
ORIGIN      
        1 cgcccggtgg gattgtacat cgcgtggacc tcgtcgtccg gaggaacgag gagcgagatc
       61 gccccgggcc cgtggcgggt cacgctctgc acggtgagcg cggcgccccc gcccgggccc
      121 tcggccaccc ggtaacgagt ctcctcgatc tcgttgtcga ccacgccgat gacgccccat
      181 gtgtcgtgat tgtgggccgc cgcgcggtcg cccggccgcc aggtcaccgc ggtgacgttg
      241 aagcgcggcg cgcggtggag catgaagcgg ccgcggccgc cgtcggggcc gcgcccgcgg
      301 aagggagccg ggatcgccgc gtcgttgcgc accaggcggc cgagcagggg cgcgacctgg
      361 gcggtgatgg cggcgggctc gctctcccgg ccggtgatgc ggtccaggtc ggccacgaac
      421 tgctcgaggg agtaggtctc ggtggccatc acgcctctcc ggtggcgacc ggcgtgtccg
      481 cgcggccgcc ccattcggtc cacgagccgt cgtagaccgc ggcgtcgcgc cggcccagac
      541 ggtagagccc gagcgccagc accgccgcgc tcacccccga gccgcagctc gccaccaccg
      601 gccgcgcggg atcgagcccg gcgcgggcga acgcggcgcc cacgtcctcg gcgggcagca
      661 tcgcgtcgtc gtcggcgcga aacagctcgg tgtagggcag gctgcggctc cccgggatgt
      721 ggcccccgcg caggccgggg cggatctcgg gctcggtggc cgcgaagcgc ccccgtgagc
      781 gcgcgtccac cacctgggcg tcgcgccgct ccaggatggc ccgcatccgg tcgaggtccg
      841 cgaccagctc gggccggtag gtggccacga accgccgcgc ggccgggctc gcgcggccgg
      901 cctcgagcgg gcggccctcc tgcttccacc tggccgagcc gccgtccagc accgcgaccc
      961 gatcgtggcc gaacacgcga aaggtccacc acgcgcgggc cgaggcgatg agcccgcgcc
     1021 cgccgtaggc gaccacgcgg tctcccgccg cgatgccgag cgcgcccatc gcgtccgcga
     1081 aggccttggc gtcgggcagc atgtgcggca gcgggttcgc gtggtcggcg atggtctcga
     1141 cgtcgaagaa gaccgcgccg ggcacgtggg cctgctcgaa ctcggccgcc gcgtcgcgct
     1201 tgagatgcgg caggtagaag gtggcgtcca ccaccttcag atccggctcg cccaggtggg
     1261 cggccagcca ctcggtggag acgagcggat ccggcagggc cgcgctcacc gggtgctccc
     1321 gggcgcggcg ggccgctccg gcagcagcgc gtgggggctc accccgtgcg cgtcgagcgc
     1381 ctcttcccag cgcaggtagg cttccatggc ggcccgcccg cgctcgtagg gcttctggac
     1441 cacgtcgtcg gcttcgccct gcatcgagcc gaggccggtc tcgaccggga gcccggcggc
     1501 gcgccacgcg gccactcccc cgtcgagcac ccgcgccgcc gcgtagccca tgcgacggag
     1561 cgtcgccgcg gcgagggtcg actgcacgcc gtcggcgcac gtgagcagca cgcgccgggt
     1621 gggatcgggg gcggcccggc cgatggcgag ctcgagccgt ccgcgcccga tccagatcgc
     1681 gccgggcaga tgcccgcgct ggtagacgtc gctcgggtcg acgctcacca cgacgtcgtt
     1741 gccgaggggc ccgggggcca cgcgctcgac cacgcgctgc gcggcctcgt agccgaacgg
     1801 ggccggcgcc ggctgtccgg tctcgagcgg gcggccggag cgctcccacg ccggcacgcc
     1861 gccctcgagc accgccacgt gcgggaagcc catgcgcttg agccaccccg cggtcatgat
     1921 cgaccggccg aagccgtcgc agatcagcac gagccatccc gcgcgcaccg ccacgtactc
     1981 gtcggtggcc tgcaccgcct gcccgcccgg cgcccagata gagcccggca cgtggccggc
     2041 ctcgtactcc tcccgggtgc gcacgtcgag catcgagacg ctctcgcgat cgcgatggct
     2101 ccatcgctcc gccaccgcgt ccggcgagac cagcggggtg ccgtcctcgg cggccagccg
     2161 cgcggcgacc tcgcgggccc gcgcccggct cgccgccgac ggggccggcg gccagcggct
     2221 cgccccgcgc tcgagctcga ggccgtcgag ctgccagccc atggtgccgt t
//
LOCUS       DATMCV010000468         2315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_891699, whole genome shotgun sequence.
ACCESSION   DATMCV010000468 DATMCV010000000
VERSION     DATMCV010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891699"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VNU03_10810"
     CDS             complement(<1..470)
                     /locus_tag="VNU03_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWP74999.1"
                     /translation="MPTLRPGDRAPDVTLPAADRDGVVALTDYRGKQPVLLALYRSLY
                     CPFCRRQMSQLALSVERLERIGVATLAVVTTAPERARLYYRFRPPRFRVAADPDLTTH
                     RAYGLPHLPKTPAIRQIIEAVAVDMARDLGIPTLPGQASDAILGFDGFEPLESDR"
     gene            complement(516..1010)
                     /locus_tag="VNU03_10815"
     CDS             complement(516..1010)
                     /locus_tag="VNU03_10815"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWP75000.1"
                     /translation="MHWLVDGYNVIRREPALAERERESLEAGRQALCRLLSNAARVSG
                     DRVTVVFDGSRAGGSFSGGPGVHAIFSSARETADHVLARMATAGAAVVSNDREVRRAA
                     ARARAIAVTTDEFLSRLERAGRGRGSDKEDEEAGRPQPKKGNPRRLSKKDRRAARALG
                     RLAR"
     gene            1186..1977
                     /locus_tag="VNU03_10820"
     CDS             1186..1977
                     /locus_tag="VNU03_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP75001.1"
                     /translation="METGLRGKIAMVSGASKGIGRAIAEKLAAEGASLSLCARSAEPL
                     RAVARQIEDKHGGTCLAVPGDLNTEEAIRGWVAATIERFGGVDILVNNAGAAQGGAFL
                     DLPDQVWRDSLNLKLFGYIRVAREVFPHLVRRGGGRIVNVIGMGGVQPMDNYMVGGAA
                     NAALVNFTKALGNEGAPHGILVTGVNPGATKTERWDTMLDKWGAAKGVTPGQAEREIL
                     DGVPLKRAGTVDEVANLVVFLASDLSSYITGTTVNVDGGMARMIF"
     gene            2007..>2315
                     /locus_tag="VNU03_10825"
     CDS             2007..>2315
                     /locus_tag="VNU03_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75002.1"
                     /translation="MRRRDFLTMAAVGGVGAGLGLPGLLRRAAAATPIKIGMPAALSG
                     SYAQYGIQAKRAAELFTKDITAKGGVLGRPVEFIYEDTAGDPATAVRKAQKLVEKDGV
                     K"
BASE COUNT          368 a          799 c          828 g          320 t
ORIGIN      
        1 cgatcgctct cgagcggctc gaatccatcg aagcccagga tcgcgtcgct cgcctggccc
       61 ggcagggtcg ggatccccag gtcacgcgcc atgtcgaccg ccacagcctc tatgatctgc
      121 cggatcgccg gcgtcttggg aaggtgcggc aacccgtacg cgcggtgcgt cgtcaggtca
      181 ggatctgcgg cgacgcgaaa gcggggggga cggaatcgat agtacaagcg cgcacgttcg
      241 ggcgccgtgg tgacgaccgc gagcgtggcg acgccgatcc gctctaaccg ctcgacgctc
      301 agcgcgagct ggctcatctg ccgacgacag aatgggcaat acagactgcg gtaaagtgcc
      361 aggagcaccg gctgctttcc ccggtagtcg gtcagggcaa cgaccccgtc tcgatccgcg
      421 gcagggaggg taacgtccgg tgctcggtcg ccgggtcgca gtgtgggcaa atcttcgctc
      481 cgcctctcac ctaccgagtc ggagggtctg ctcgctcatc tcgcaaggcg gcccagcgcg
      541 cgggccgcgc ggcggtcctt cttgctcaaa cggcgcgggt tgcccttctt gggctggggc
      601 cggcccgctt cctcgtcctc cttgtccgaa ccgcgcccgc ggcccgcccg ctccaggcga
      661 gagagaaact catcggtggt gacggcgatg gcccgggcgc gggcggccgc ccgccgcacc
      721 tcccggtcgt tggagacgac cgcggccccc gccgtggcca tccgcgccag cacgtgatcg
      781 gccgtctccc gcgcgctgga aaaaatggcg tgcacgccag ggccgccgga aaagctaccg
      841 cccgctcggc tcccgtcgaa gacaacggtg acccgatcgc ccgacacgcg cgcggcattc
      901 gagaggaggc ggcagagcgc ctggcgtccg gcctccaagc tctcccgctc gcgctcggcc
      961 agcgcgggct cccggcggat cacgttgtag ccatccacga gccagtgcat gggtaattta
     1021 ctctctccta gccggcgctc accaagcaag aggggacttc aacgcaccct catatgctcc
     1081 agtccgaacg gttgtcaacg ccgggactgg gcgcgccgct tgacaggcct acgtcgccgg
     1141 agcactatcg cgcccgatag ttgggcggga gcggaaggag acgggatgga gaccggcctg
     1201 cgcggcaaga tcgcgatggt gtcgggcgcg agcaagggga tcgggcgcgc gatcgcggag
     1261 aagctcgccg ccgagggggc gagcctgtcg ctgtgcgccc gcagcgccga gccgctgcgc
     1321 gcagtggcgc gccagatcga ggacaagcac ggcggcacct gcctcgccgt tcccggcgac
     1381 ctgaacacgg aggaagcgat ccggggctgg gtcgcggcca ccatcgagcg cttcggcggc
     1441 gtcgacatcc tcgtcaacaa cgcgggggcc gcgcagggcg gcgccttcct cgacctgccc
     1501 gaccaggtct ggcgcgacag cctgaacctg aagctcttcg gctacatccg ggtggcgcgc
     1561 gaggtcttcc cgcatctcgt gcggcggggc ggcggacgca tcgtcaacgt catcggcatg
     1621 ggcggtgtgc agccgatgga caactacatg gtgggcggcg ccgccaacgc cgccctcgtg
     1681 aacttcacca aggcgctcgg caacgagggc gcgccccacg gcatcctggt caccggcgtg
     1741 aaccccggcg cgacgaagac cgagcgctgg gacaccatgc tcgacaagtg gggcgcggcc
     1801 aagggcgtga cgcccggcca ggccgagcgc gagatcctcg acggggtccc gctcaagcgc
     1861 gcgggcaccg tggacgaggt ggccaacctc gtggtgttcc tcgcttccga tctgtcatcg
     1921 tacattaccg gcacgacggt caacgtggac ggcggcatgg cccggatgat cttctgatgc
     1981 ggccgggccg cgaaaagggg acgaccatgc ggagacgcga cttcctgacg atggcggcgg
     2041 tgggcggcgt gggcgcgggc ctcgggctcc cggggttgct ccgccgcgcg gccgcggcca
     2101 cgcccatcaa gatcggcatg ccggccgcgc tgtccggcag ctacgcgcag tacggcatcc
     2161 aggccaagcg ggcggccgag ctcttcacca aggacatcac ggccaagggg ggcgtcctgg
     2221 gccggccggt ggagttcatc tacgaggaca ccgcgggcga tccggccacc gcggtgcgca
     2281 aggcccagaa gctggtggag aaggacggcg tgaag
//
LOCUS       DATMCV010000469         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_427032, whole genome shotgun sequence.
ACCESSION   DATMCV010000469 DATMCV010000000
VERSION     DATMCV010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427032"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /locus_tag="VNU03_10830"
     CDS             <1..1983
                     /locus_tag="VNU03_10830"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP75003.1"
                     /translation="MRADAPLLYPALGTNVVWHDTLTYGDVDGALARADGVLRERFTI
                     QRYASTPLETFGAIAEYDAGTDAFEFWTNDQRPGLTISILAASLGVPQSRIRLHCPDI
                     GGAFGNKRRPAYILVCALLARAAGRPVKWIEDRIENLTALMHACNGVMDVALAYRADG
                     TLLALDVRDVADEGKNLLTPTQHNLIKLGNIANGYRIPAVRYEAWSVLTNKCPSGANR
                     GIGKPFMCFAVERAMALLARRLGLDPAELRRRNYVTAEQMPYTTPPGAQYDSGDYPAT
                     LERALERFDYAGWRAEQARARAEGRLLGIGIATSVEPAGTNLASYELVTGRRAASGSA
                     EAAMVRVEPDGHVRVSLGDPSSGQGYETVVAQIVADELGVTPEAVSVARGFDSATTPW
                     LYLSGNYSNKFSVTDVGAVVGAAARVRAKLLRIAAHRLEIDVADLELRDGAAVVRGAP
                     DRRLAIAELARTAYADVLGLPPGVDPGLEAHHIHQNPLAQPVDADRRVRSQLVFANAA
                     HCCLVEIDPRIGRITLRKYVVVHDCGRELNPLIVEGMVHGSTVHGIGAALLEEFRYDE
                     TGQLLTSTFMDYLKPTAADVPTIEVDRLEHPSPVTPLGAKGVGEGGSIPGPAAVANAV
                     EDALAHLGVVVRSLPITPEQVWRWLRAAKPVGSREA"
     gene            1980..>2339
                     /locus_tag="VNU03_10835"
     CDS             1980..>2339
                     /locus_tag="VNU03_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HWP75004.1"
                     /translation="MRTRRRFLGTLGALALAAPVIAEAQPAGRVPHVAVLNPGSATEA
                     PAVQREPFERGLRELGWRPGATVVIEYVYGDGSASQLATVTANLVRRGVEVIVARGPV
                     AIRAAREATTTVPIVMSA"
BASE COUNT          312 a          861 c          865 g          301 t
ORIGIN      
        1 atgcgcgcgg acgccccgct gctctatccc gcgctcggga ccaacgtggt ctggcacgac
       61 acgctcacct acggcgatgt ggacggcgcg ctggcccgcg ccgacggggt gctgcgggag
      121 cgcttcacga tccagcgcta cgcctccacg ccgctcgaga ccttcggggc gatcgcggag
      181 tacgacgcgg gcacggacgc tttcgagttc tggaccaacg accagcgccc ggggctgacc
      241 atctcgatcc tggccgcctc gctcggcgtc ccgcagtcgc ggatccgcct gcactgcccc
      301 gacatcggcg gcgccttcgg caacaagcgc cggccggcct atatcctcgt ctgcgcgctg
      361 ctggcccgcg cggccggccg gccggtgaag tggatcgagg accgcatcga gaacctcacc
      421 gcgctcatgc acgcctgcaa cggggtcatg gacgtcgcgc tcgcctaccg ggccgacggc
      481 accctgctcg cgctcgacgt ccgcgacgtc gcggacgagg gcaagaacct gctcaccccg
      541 acccagcaca acctgatcaa gctcggcaac atcgccaacg gctaccggat ccccgcggtg
      601 cgctacgagg cgtggtcggt gcttaccaac aagtgcccga gcggggccaa ccgcggcatc
      661 ggcaagccgt tcatgtgctt cgcggtggag cgggccatgg ccctgctggc gcgccggctc
      721 gggctcgacc cggcggagct gcgccggcgc aactacgtga ccgcggagca gatgccctac
      781 accacgcccc cgggggccca gtacgacagc ggcgactacc cggcgacgct cgagcgcgcc
      841 ctcgagcggt tcgactacgc cggctggcgg gccgagcagg cccgcgcgcg ggccgagggc
      901 cggctgctgg gcatcggcat cgccacgtcg gtggagccgg cgggcaccaa cctcgcctcc
      961 tacgagctgg tgaccgggcg gcgcgccgcg tcgggctcgg cggaggcggc gatggtccgg
     1021 gtggagcccg acggccacgt gcgggtgagc ctgggcgacc cgtcgagcgg ccagggctac
     1081 gagacggtgg tggcgcagat cgtcgccgac gagctggggg tgacgccgga ggcggtcagc
     1141 gtcgcccgcg gcttcgactc ggcgaccacc ccctggctct atctctccgg gaactactcc
     1201 aacaagttct cggtgaccga cgtgggcgcg gtggtgggcg cggcggcgcg ggtgcgcgcc
     1261 aagctgctgc ggatcgccgc gcaccgcctg gagatcgacg tggcggatct ggagctgcgc
     1321 gacggcgccg cggtggtgcg cggggcgccc gaccggcggc tcgcgatcgc ggagctggcc
     1381 cgcaccgcct acgcggacgt gctcggcctg ccgcccggcg tggacccggg cctggaggcc
     1441 caccacatcc accagaaccc gctggcccag ccggtcgacg cggaccgtcg ggtacgctca
     1501 cagctggtct tcgccaacgc cgcccactgc tgcctcgtcg agatcgaccc gcgcatcggg
     1561 cggatcacgc tccggaagta cgtggtggtg cacgactgcg gccgggagct caaccccctg
     1621 atcgtcgagg gcatggtgca cggctcgacg gtgcacggca tcggcgccgc gctcctcgag
     1681 gagttccgct acgatgagac cggccagctg ctcacgtcga cgttcatgga ctacctcaag
     1741 cccaccgcgg ctgacgtgcc caccatcgag gtcgaccggc tcgagcaccc gtcgccggtc
     1801 acgccgctcg gcgccaaggg ggtgggggag ggcggctcga tcccgggtcc ggccgcggtg
     1861 gccaacgcgg tggaggacgc gctcgcgcac ctgggggtcg tggtccgctc gctgccgatc
     1921 accccggagc aggtctggcg ctggctacgc gcggcgaagc cggtcgggtc gcgagaggca
     1981 tgaggacccg acgccggttt ctcggcacgc tcggcgctct ggcgctggcc gcgccggtca
     2041 tcgcggaggc gcagcccgcg gggcgcgtcc cgcacgtcgc ggtgctcaac ccggggagcg
     2101 ccaccgaggc gcccgcggtg cagcgcgagc cgttcgagcg cggcctgcgg gagctgggct
     2161 ggcggcccgg cgcgacggtg gtgatcgagt acgtgtacgg cgatgggagc gccagccagc
     2221 tcgccaccgt gaccgcgaac ctcgtccgga ggggcgtcga ggtcatcgtg gcccgcgggc
     2281 cggtggccat ccgcgcggcc cgcgaggcga cgaccaccgt tcccatcgtc atgtcggcc
//
LOCUS       DATMCV010000470         3557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_359456, whole genome shotgun sequence.
ACCESSION   DATMCV010000470 DATMCV010000000
VERSION     DATMCV010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359456"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VNU03_10840"
     CDS             complement(<1..1285)
                     /locus_tag="VNU03_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75005.1"
                     /translation="MRPADHVSRRNFIQAGALGVAGLAAVRPRVAWAQNGEAKRGGSL
                     VVRSGPLRGIDPHLETWAAALQVIHQTYNALLKFNHDGTKILPDLAESWEQKDDLTYS
                     FKLRQGVKFHNVPPVNGRELTSEDVKYSIERQMTDQPGKFQHAYFFLGKLASIQTPDK
                     YTIVFKTKEPYAPFLNYIANPWTVIVAREVVEKHGDLQRHAIGTGPFVMQEYRREQEA
                     VFVKNPNYFKKGLPYLDEVRIQYVIDPVAATAAFRGQQTDISLLEFADVPSIKASNAK
                     IKVAEITTMFPIVLRTRPYDEQRPLKPPFTEKKVRQAIQAAIKQEEFIEIVRSGWGTT
                     MTGPIPTYRKPWALPASARPEYDPKKAKRLLTEAGYANGFETELIVGSPGDNVKSAQV
                     VKDQLARVGIQVNLKPMEMAQYYNKSYTYDYSMSVH"
     gene            complement(1413..2102)
                     /locus_tag="VNU03_10845"
     CDS             complement(1413..2102)
                     /locus_tag="VNU03_10845"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWP75006.1"
                     /translation="MTSDELKAWRKAERERLIGARERLDPATLEGWRRRIDSHLQRSF
                     PGLATAKLAFCWPMRGEYDARPLADTLRARGAVTALPVVVAAGQPLVFRQWHPGVALA
                     LGPLDIPYPADSDPVVPTVALVPVNGWDDAGHRLGYGAGFFDRTLASPPAPVVAIGVG
                     YELARVKTIHPQAWDVPMDWIVTERGVYRRDPSGLSFLGEPDRGDPGPLASPVCYVDE
                     IAPDHYRDPER"
     gene            complement(2123..>3557)
                     /locus_tag="VNU03_10850"
     CDS             complement(2123..>3557)
                     /locus_tag="VNU03_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP75007.1"
                     /translation="CYHDSMSDDRAARTRAHWLDTLARYRRDPDRPGSADYWSPSLDC
                     AGRDELRAIQDAKLAALTPFLYENSAFYRRRFDRLGLLPTDLRTVDDLPRWPVVDKTE
                     MMEDLKAAPPFGTYTTHDDALWAARGWMMFSSSGSTGVPRVFRYSQIDRELWEWANAR
                     ALHAFGIRPGDTVLICAGYGPHVFAWGVQFALARMGVACIPGGGMTAEARAMLVDRFK
                     PTVLTCTPSYALHLGRVMAERGLDPAASSIRTILVGGEPAAGIEGTRRRLEDLWSARL
                     VEFYGCTEVSPHCGGYSCPASARGPGPVTTHLMEDVQIWELVDAESRRPVAEGDRGLT
                     VCTSLNSESSPQLRFLVGDYAVLTSERCACGRTHVRAVGGFAGRADELINLRGVKMYP
                     VQLEAAVRAVPGVGDEYEIVLATTPDGLDVMTARVEHPSGSIGAQVAREIQVRCEIRC
                     DVEVLAPGTLPKTEFKARRIRDTRRAG"
BASE COUNT          500 a         1212 c         1277 g          568 t
ORIGIN      
        1 tgtgcacgct catggagtag tcgtacgtgt agctcttgtt gtagtactgg gccatctcca
       61 tgggcttcag gttgacctgg atgccgaccc gggcgagctg atccttcacc acctgggcgc
      121 tcttcacgtt gtcgcccggg ctgcccacga tgagctcggt ctcgaagcca ttggcgtagc
      181 cggcctcggt gagcaggcgc ttggccttct tgggatcgta ctccggccgc gccgacgccg
      241 gcagggccca cggcttgcga taggtgggga tggggccggt catggtcgtg ccccagccgc
      301 tccgcacgat ctcgatgaac tcctcctgct tgatcgcggc ctggatggcc tggcgtacct
      361 tcttctcggt gaaggggggc ttgagcgggc gctgctcgtc gtagggccgg gtccgcagca
      421 cgatcgggaa catcgtggtg atctcggcca ccttgatctt ggcgttcgag gccttgatcg
      481 agggcacgtc cgcgaactcc agcagggaga tgtcggtctg ctgcccgcgg aacgcggcgg
      541 tggccgccac cggatcgatg acgtactgga tacggacctc gtccaggtag ggcagcccct
      601 tcttgaaata gttggggttc ttgacgaaca ccgcctcctg ctcgcgccgg tactcctgca
      661 tcacgaaggg cccggtgccg atcgcgtggc gctgcaggtc gccgtgcttc tccaccacct
      721 cgcgcgccac gatcacggtc caggggttgg cgatgtagtt caggaacggc gcgtacggct
      781 ccttggtctt gaacacgatc gtgtacttgt ccggcgtctg gatgctggcc agcttgccca
      841 ggaagaagta ggcgtgctgg aacttcccgg gctggtccgt catctgacgc tcgatgctgt
      901 acttgacgtc ctccgacgtg agctcgcgcc cgttcaccgg gggcacgttg tggaacttga
      961 cgccctggcg cagcttgaac gagtaggtga ggtcgtcctt ctgctcccag ctctcggcca
     1021 ggtccggcag gatcttggtg ccgtcgtggt tgaacttcag gagggcgttg tacgtctggt
     1081 ggatcacctg gagggcggcc gcccaggtct ccagatgcgg gtcgatgccc cgaaggggcc
     1141 cgctccgcac gaccagggag ccgccacgct tggcctcgcc gttctgggcc cacgcgaccc
     1201 ggggtcggac ggcggccagt cccgccacgc cgagggcgcc cgcctggatg aagttccgtc
     1261 gcgacacgtg atcggcaggc ctcatgtcga ctccccctgt tctccgttga ccaggtgcgt
     1321 ctgagccgct cggccgtcgc cggccgctcg gtggtggcag ggaggatggc ttgtccgccg
     1381 atcagagtca aggaggccgg agcgggcgcg gactatctct ccgggtcgcg gtagtgatcc
     1441 ggggcgatct cgtccacgta acagaccggg gaggccaggg ggcccgggtc gccccggtcg
     1501 ggctcaccga ggaaggagag cccgctcggg tcgcgccgat atacaccacg ctcggtcacg
     1561 atccagtcca tcggcacgtc ccaggcctgg ggatggatcg tcttcacccg agccagctcg
     1621 tagccgacac cgatggcgac gacgggcgcg ggcgggctcg ccagggttcg gtcgaagaag
     1681 ccggccccgt accccaggcg atggccggcg tcgtcccagc cgttcaccgg cacgagcgcg
     1741 acggtgggga cgacggggtc ggagtcggcc ggatagggga tgtcgagggg cccgagagca
     1801 agcgccaccc cgggatgcca ttgccggaag acgagcggct gtccggcggc gacgaccacc
     1861 ggcagcgcgg tcaccgcgcc ccgcgcgcgg agcgtgtcgg cgagagggcg cgcatcgtat
     1921 tccccgcgca tcggccagca gaaggccagc ttcgcggtgg cgaggccggg gaacgagcgc
     1981 tggaggtgcg agtcgatccg ccggcgccag ccctcgaggg tcgcggggtc gagccgctcg
     2041 cgcgcgccga tgagccgctc acgctcggcc ttccgccagg ccttcagctc atcggacgtc
     2101 atgcgggctc cgggctcccg gctcagccgg cgcggcgcgt gtcgcggatc cgccgcgcct
     2161 tgaactcggt cttgggcagc gtgccgggcg ccaggacctc gacgtcgcac cgtatctcgc
     2221 accgcacctg gatctcccgg gccacctgcg cgccgatcga gccgctcggg tgctccaccc
     2281 tcgcggtcat cacgtcgagc ccgtccgggg tggtcgccag cacgatctcg tactcgtcgc
     2341 ccactcccgg taccgcccgc accgccgcct cgagctggac ggggtacatc ttcaccccgc
     2401 gtaggttgat gagctcgtcg gcccgccccg cgaagcctcc caccgcccgg acgtgggtcc
     2461 ggccgcaggc gcagcgctcc gaggtgagga ccgcgtagtc gccgaccagg aaccgcagct
     2521 gcggcgagct ctcggagttg aggctcgtgc agacggtgag gccgcggtcg ccctctgcga
     2581 ccggccgccg gctctccgcg tcgaccagct cccagatctg cacgtcttcc atcaggtggg
     2641 tggtgaccgg ccccggtccc cgcgccgagg ccgggcagga gtagccgccg cagtggggcg
     2701 agacctcggt gcagccgtag aactccacga gccgtgcgct ccatagatcc tcgaggcgcc
     2761 ggcgcgtgcc ctcgatgccc gcggcgggct cgccgccgac caggatcgtc cggatcgagc
     2821 tcgcggccgg gtcgagcccg cgctccgcca tcacgcggcc caggtggagg gcgtaggagg
     2881 gagtgcacgt gaggacggtg ggcttgaagc gatcgacgag catcgcgcgg gcttccgcgg
     2941 tcatgccgcc gcccgggatg caggccaccc ccatccgggc gagcgcgaac tgcacgcccc
     3001 aggcgaacac gtgcggcccg tacccggcgc agatcagcac cgtgtcgccc gggcggatgc
     3061 cgaaggcgtg gagcgcccgt gcgttcgccc actcccagag ctcgcggtcg atctgggaat
     3121 agcggaagac cctcggcacg ccggtcgagc ccgaggacga gaacatcatc cagccgcgcg
     3181 cggcccacag ggcatcgtcg tgcgtggtgt aggtgccgaa cggcggcgcg gccttgagat
     3241 cctccatcat ctcggtcttg tcgacgaccg gccacctcgg cagatcgtcc accgtgcgca
     3301 ggtcggtggg cagcaggccg agccggtcga agcggcgacg gtagaacgcg ctgttctcgt
     3361 agaggaaggg agtcagcgcg gcgagcttcg cgtcctggat ggcccggagc tcgtcgcggc
     3421 ccgcgcagtc gagggagggc gaccagtagt cggcggagcc ggggcgatcc ggatcgcgcc
     3481 ggtaccgggc gagggtgtcc agccagtggg cgcgtgtgcg cgccgcccga tcgtcactca
     3541 tggagtcgtg atagcat
//
LOCUS       DATMCV010000471         3709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_359513, whole genome shotgun sequence.
ACCESSION   DATMCV010000471 DATMCV010000000
VERSION     DATMCV010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359513"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VNU03_10855"
     CDS             <1..542
                     /locus_tag="VNU03_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP75008.1"
                     /translation="YFDNVRVPASQILGGLDNGWAVGLTTLMYERLALGFGLQVRLRI
                     ALDGLVDMARATKKNGIPVTQDPVMRQKLAQMWIDTEVFKYTGARAITKLLKGELPGP
                     EASAGKMMWVEGHQRVQELAMEIQGAFSQLTHGSAWAVADGLWQHTFLRSRANSIEGG
                     TTEIQRNIIGERVLGLPKG"
     gene            546..1682
                     /locus_tag="VNU03_10860"
     CDS             546..1682
                     /locus_tag="VNU03_10860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP75009.1"
                     /translation="MNFGFNDEQELLRSTARKFFENECGSDTVRRLMETPEGISPDLW
                     TKLAEQGWLGLVYPEAYDGMGLGLVDLVVLMEEMGRAVVPGPYFSAVLLGGLAIAEAG
                     SEAQKKEWLPKIAAGDRRVALAWMEPSAQLGPAGVTLTAGEKGGKYTLSGTKLFVHDA
                     HTADALVVAARTRPGAGADGVSLFLLPKGTKGLEVTLLPTMDQTRKLCEVALSDVTVG
                     PDALLGAAGAGWAPLARVLDRATVALCAEMCGGAQKVLDMTVEYAKIRQAFGRPIGSY
                     QGVKHRAADMLVDVENSKSITYYAAWALDENAADAPLAVSMAKAYVSDAYRRVAAAGI
                     QLHGGIGFTWEHDLHLYFKRAKGSEFTFGDATHHRERVAQLVNL"
     gene            1698..3032
                     /locus_tag="VNU03_10865"
     CDS             1698..3032
                     /locus_tag="VNU03_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75010.1"
                     /translation="MIKRVREQMGRWIRRDPRELPPEALLFADHEEIQDIARQIRRTK
                     VEMDRAVLAMRSWARDLGRRLAEAPRGLSRGEDLRVLGETVGGLAHNFNNSLAAILAY
                     TELMLRESESEAARRRLVVIRDVALEASATVRKLQEFVSRQPQVAFGPVGLPAVIGEA
                     LELTAPRWRDEAERKGTVIAVTQDMEALPPVEGNAFELRDALVLLIHNAVDAMPKGGS
                     LAIRAAAEASGWVVVEVRDSGVGMSDETRRRLAEHARSLQPGRGSSQGLAHVYDIVER
                     HGGSVAIESEIGKGTTVRLRLHASRFQIIPASEGLAERQVSPDQAARVLLVDDDPRLL
                     SVLSDVLSTEGHAITTAANGEEALAVFDPGAHDVVITDLGMPRMNGWEVAQRVKARSP
                     GTAVFILTGWGEGVSAHESMQFVDRVIAKPVSAEALLEQLAGLTRSRVVPPA"
     gene            3029..>3709
                     /locus_tag="VNU03_10870"
     CDS             3029..>3709
                     /locus_tag="VNU03_10870"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWP75011.1"
                     /translation="MTALAGSPVAPRPAASVLLVRPGTSCPVEVYMIRRQKSMRFLGG
                     FYAFPGGKVEASDGSEAAFARCFGVSSATALAAFPPTDGLPPLAFWVTAARELVEETG
                     LLVGCDADGRPVDTRDPAVRERVERVRHALAADGASFHALLEEEGWHLDLAPFRYLSH
                     FITPPSSPIRFTARFFLAPVPAGQEPRLLGGEASEGFWIDPAEGHSRFLAGEMQMAEP
                     ADCGLRYLS"
BASE COUNT          518 a         1351 c         1327 g          513 t
ORIGIN      
        1 tctacttcga caacgtccgc gtgcccgcct cccagatcct gggcggtctc gacaacggct
       61 gggcggtcgg cctcaccacc ctgatgtacg agcggctcgc gctcggcttc ggcctgcagg
      121 tccgcctgcg catcgcgctc gacggcctgg tcgacatggc gcgcgccacc aagaagaacg
      181 ggatcccggt cacccaggac ccggtgatgc gccagaagct ggcccagatg tggatcgaca
      241 ccgaggtgtt caagtacacc ggggcgcgcg cgatcaccaa gctgctcaag ggcgagctgc
      301 ccggtcccga ggcctccgcg ggcaagatga tgtgggtgga gggccaccag cgcgtgcagg
      361 agctggccat ggagatccag ggcgcgttca gccagctcac ccacggcagc gcgtgggcgg
      421 tggccgacgg cctctggcag cacaccttcc tgcgctcgcg ggccaactcg atcgagggcg
      481 gcaccaccga gatccagcgc aacatcatcg gcgagcgcgt gctcggcctc ccgaaaggct
      541 aagccatgaa cttcggcttc aacgacgagc aggagctgct gcggagcacc gcgcgcaagt
      601 tcttcgagaa cgagtgcggc tcggacacgg tgcggcgcct catggagacg ccggagggca
      661 tcagccccga tctctggacg aagctcgccg agcagggctg gctcggcctc gtctaccccg
      721 aggcctacga cggcatgggg ctgggcctcg tcgacctagt ggtcctgatg gaggagatgg
      781 ggcgcgcggt ggtgcccggc ccctacttct cggcggtact cctgggcgga ctcgccatcg
      841 ccgaggcggg cagcgaggcg cagaagaagg agtggctgcc gaagatcgcg gcgggggacc
      901 ggcgggtggc gctcgcgtgg atggagccga gcgcgcagct cggccccgcc ggggtcacgc
      961 tgaccgcggg ggagaagggc gggaagtaca cgctctccgg caccaagctc ttcgtgcacg
     1021 acgcccacac cgcggatgcc ctcgtggtgg cggcgcggac gcggccgggg gcgggcgcgg
     1081 acggggtgtc gctcttcctc ctgccgaagg gcacgaaggg gctcgaggtc acgctgctcc
     1141 cgaccatgga ccagacccgc aagctctgcg aggtcgccct ctccgacgtc acggtcggcc
     1201 ccgacgcgct gctcggggcg gccggagccg gctgggcgcc gctcgcccgc gtgctcgatc
     1261 gggccaccgt cgcgctgtgc gccgagatgt gcgggggcgc ccagaaggtc ctcgacatga
     1321 cggtggagta cgccaagatc cgccaggcct tcggccggcc catcggctcc taccagggtg
     1381 tcaagcaccg cgcggccgac atgctggtgg acgtcgagaa cagcaagtcc atcacgtact
     1441 acgcggcgtg ggcgctcgac gagaacgccg cggacgcgcc gctggcggtg agcatggcca
     1501 aggcctacgt ctccgacgcc taccggcgcg tggccgcggc gggcatccag ctccacgggg
     1561 gcatcggctt cacctgggag cacgacctgc acctctactt caagcgcgcc aagggctcgg
     1621 agttcacctt cggggatgcc acccatcatc gcgagcgcgt cgcccagctc gtgaacctgt
     1681 aacgcgcccg cgcttccatg atcaagcggg tccgggagca gatgggccgg tggattcgcc
     1741 gcgacccgcg cgagctgccg ccggaagccc tcctcttcgc cgatcacgag gagatccagg
     1801 acatcgcgcg ccagatccgc cgcaccaagg tggagatgga ccgcgcggtc ctcgcgatgc
     1861 gcagctgggc gcgcgacctc gggcgccgcc tggccgaggc cccgcgcggg ctctcccggg
     1921 gcgaggacct ccgcgtcctc ggcgagacgg tgggcgggct cgcccacaac ttcaacaact
     1981 ccctcgccgc gatcctcgcc tacaccgagc tcatgctgcg cgagagcgag tcggaggcgg
     2041 cgcggcgccg gctcgtggtg atccgcgacg tggccctcga ggcctccgcc acggttcgca
     2101 agctgcagga gttcgtctcc cgccagccac aggtcgcgtt cgggccggtg ggcctgcccg
     2161 cggtcatcgg cgaggcgctc gagctgaccg ccccccgctg gcgcgacgag gcggagcgca
     2221 agggcaccgt catcgcggtc acccaggaca tggaggcgct gcccccggtc gagggcaacg
     2281 ccttcgagct acgcgacgcc ctcgtgctcc tcatccacaa cgcggtcgac gccatgccca
     2341 agggcggctc gctcgcgatc cgggccgccg cggaggcgtc cggctgggtg gtggtcgagg
     2401 tccgcgactc gggagtcggc atgtccgacg agacgcgccg gcgcctggcc gagcacgcgc
     2461 ggagcctgca gcccgggcgc ggctcgagcc agggcctcgc ccacgtctac gacatcgtcg
     2521 agcgtcacgg cggtagcgtg gccatcgaga gcgagatcgg caagggcacc accgtgcgcc
     2581 tccggctgca cgcgagccgc ttccagatca tccccgcctc ggaagggctc gccgagcgtc
     2641 aggtctcccc ggaccaggcc gcgcgcgtgc tcctggtgga cgacgacccg cggctcctca
     2701 gcgtgctctc ggacgtgctg agcaccgagg ggcacgcgat cacgaccgcc gccaacgggg
     2761 aggaggcgct cgccgtcttc gacccgggcg ctcacgacgt ggtgatcacc gatctcggca
     2821 tgcccaggat gaacggctgg gaggtcgccc agcgggtcaa ggcccgctca cccggcaccg
     2881 cggtcttcat cctgaccggc tggggcgagg gcgtctccgc gcacgagtcc atgcagttcg
     2941 tggaccgcgt catcgccaag ccggtgtcgg cggaggctct gctggagcag ctcgccgggc
     3001 tcacgcggtc gcgcgtcgtt ccgcccgcgt gaccgcgctc gcgggctccc cggtcgctcc
     3061 gcgccccgcc gcctcggtcc tgctcgtccg ccccgggacg agctgcccgg tcgaggtgta
     3121 catgatccgc cgccagaaga gcatgcgctt cctcggcggc ttctacgcct tccccggcgg
     3181 caaggtggag gcgtccgacg gcagcgaggc ggccttcgcg cgctgcttcg gcgtctcgag
     3241 cgcgaccgcg ctggccgcct ttccgccgac cgacggcctt ccgccgctcg ccttctgggt
     3301 gaccgcggcg cgcgagctgg tggaggagac cggcctcctc gtgggctgcg acgccgacgg
     3361 ccgtccggtc gacacgcgcg atccggcggt tcgcgagcgg gtggagcggg tacgccacgc
     3421 gctggctgcc gacggcgcct cgttccacgc cctgctcgag gaggaagggt ggcacctcga
     3481 cctggcgccg ttccggtatc tctcccactt catcaccccg ccgtcgagcc ccatccgctt
     3541 caccgcgcgt ttcttcctgg ccccggtgcc cgcggggcag gagccccggc tgctgggcgg
     3601 cgaggcctcc gagggcttct ggatcgatcc cgccgagggc cacagccgct tcctcgcggg
     3661 cgagatgcag atggccgagc cggcggactg cgggctccgc tatctctcg
//
LOCUS       DATMCV010000472         7654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_687902, whole genome shotgun sequence.
ACCESSION   DATMCV010000472 DATMCV010000000
VERSION     DATMCV010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687902"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1186
                     /locus_tag="VNU03_10875"
     CDS             173..1186
                     /locus_tag="VNU03_10875"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75012.1"
                     /translation="MDRRTFVCALTGGLLAAPLAAEAQQTGKMARVGILGIGPAPSPQ
                     ELAKSVSTNPFWLSMRHLGWVEGQNMVVERRFGESADQLRTAAADLVRLKVDVLYVAS
                     AGLAKILHLETKTIPIVVGRADSDLVAGGLVDSLARPGGNITGSQLLSDDLTPKRLEL
                     LKALVPNLSRAALLQEDVTVSAVPQTRAWSEQGAARGAMTLGIELHTFTVHRPADIAA
                     AFLGMVKNRDQAVLVGASPFVFVHRKAIIDLAAEHRIAAIYSLQVYVEPGGLMSYGVN
                     VPEMQQRAAVYVDKILKGAKPADLPIQQPTKFDLVINLKTAKALGLTIPASLLQRADQ
                     VIE"
     gene            1213..2169
                     /locus_tag="VNU03_10880"
     CDS             1213..2169
                     /locus_tag="VNU03_10880"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75013.1"
                     /translation="MTGGLLAAPLVAEAQQAGKVYRIGFLGNSTEALEANLVGPFREG
                     LRQRGYVEGRDLIIEYRWADGAYERFPALIAELIALKVDIIVTAGTPAALAVKRTTTT
                     IPLIMVAVGDPVGTGLVKSLARPGGNLTGLVAIAPDLEGKRLELLTQIVPKLSSVAFL
                     GNPANPIHVTSEKQASAAAKALHLKVVFYPVRVEGEFDHAFQAIVGQRSGALVMLADR
                     FFLHHRARIVEFTARHRLPAVYAYNELVQVGGLMSFGPSYPEMHRRAAYYVDRILKGA
                     KPGDLPIEQPTKFELVINLKTAKALGLTIPPSLLQRADQVIE"
     gene            2406..2855
                     /locus_tag="VNU03_10885"
     CDS             2406..2855
                     /locus_tag="VNU03_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWP75014.1"
                     /translation="MTAQLQRPYLASYCMIQANGTILVQRRFNTGYLDGHWALPSGHA
                     IDGEDALTAASRELLEETNLIVKPEDWQFVCAMHRRTDRTIIDLFFTTDTFVGIPRIC
                     EPTKCDGLAFFPMNALPQPFAGYVGVALDCLRGISPRHGPYYSEGWP"
     gene            complement(2883..3635)
                     /locus_tag="VNU03_10890"
     CDS             complement(2883..3635)
                     /locus_tag="VNU03_10890"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWP75015.1"
                     /translation="MRLAGKVALISGGARGMGAAEARLFAREGARVVLGDLLDAEGKQ
                     VEAAITGAGGQAVYVHLDVTSETDWEQAVATAVSRFGRLDVLVNNAGVGGGSRLEDTT
                     AEAWDHGMDVNAKGVFLGTKAAIPAMRRAGGGSIVNISSQLGMVGMERSSPQYQASKG
                     AVRTLTKLTAVQYARERIRANSVHPGPVVTPMTEARRGDPGHYEYMVSRIPLGRYGEP
                     DEVAYGVLYLASDEAAFVTGSELVIDGGWTAQ"
     gene            complement(3632..4252)
                     /gene="upp"
                     /locus_tag="VNU03_10895"
     CDS             complement(3632..4252)
                     /gene="upp"
                     /locus_tag="VNU03_10895"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HWP75016.1"
                     /translation="MVVIRHPLVQHKLGLMRKKDTPTSVFRSLLSEISMLLAYEVTRD
                     LPTHEVAAETPVAPTTIRLLDGKKIVLVSILRAGNGLLDGMLRILPSARVGHVGLYRD
                     AATLQAVEYYFKLPHEMAERDVVVLDPMLATGNSAIAAVTRVRATHPRSIRFVCLLTC
                     PEGLAAFHRAHPDVPVYTAAIDSHLNEHGYIVPGLGDAGDRLFGTR"
     gene            complement(4307..5245)
                     /locus_tag="VNU03_10900"
     CDS             complement(4307..5245)
                     /locus_tag="VNU03_10900"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP75017.1"
                     /translation="MSLTTRALHPLFGAELVGLDVTREDDATFAEIVAAFEEHSVLLF
                     RGQSLSDEAQIAFSRRFGPLETTIRSIASREGTPIHISNLSNVDGENRLIPTGDKRNV
                     YNAGNQMWHTDSSFKKVPAHASLLSAREIPPEGGETQFASMRVAHARLPVDMQAFLAD
                     KVAVHSFVYSRGLVDDGLMPPDHAAQVPPVRQALVRANPVNGRRAFYVGSHACEIVGM
                     PTAEARALLRELREAATRPELVYTHRWRVGDLVMWDNRCMLHRGRPWDESRHRRVMHR
                     TTVAGAGPTAPDEAPAAFSPARAEDVAWGRAQLELV"
     gene            5355..5819
                     /locus_tag="VNU03_10905"
     CDS             5355..5819
                     /locus_tag="VNU03_10905"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP75018.1"
                     /translation="MSTVESPVTIVVNRLALGPTAPDAGSPLLTRVGEVVAQMGPPPW
                     SKRIIADERNLVTLIASPPGGGNRPHWHREFDEWWVVLGGRLQWELTGGMVVNAAKDD
                     IVWVPRGTVHHIRNVGDELSLRLAVAMPPAWHIYHPCEQCGFADDGPREWCA"
     gene            complement(5830..>7654)
                     /locus_tag="VNU03_10910"
     CDS             complement(5830..>7654)
                     /locus_tag="VNU03_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75019.1"
                     /translation="VGGAIAWLLAAKLFSRVDYTLILYAALAPGFVVGAFLLAATALV
                     GLLSRWTEDDDREWWSRCGAWFLIAGSAWTVFGALVLFGPVLFSDVWISRALTAVGGV
                     SGLMAVVGGWFTKLGARDRASSQTSPRDRLMALAVPLAGVVFAAVLAIGLSTLISGLL
                     GWLIRQSGDPVGGLPQWGNWPHGPFDHVGVLVSSPIWIVAGVAMALAALGLLMAWFIN
                     TNRFSLHAMYRNRLIRAYLGASNTDRHANPFTGFDDNDNLQLHELWPRSSVAPAGAAV
                     PRRRLLHVVNMALNLVHGERLAWQERKAHTFTASALHAGSLMVGYRRTRAGVGGPRAH
                     YGGTEGMSLGTAVTISGAAASPNMGYHSSPLVTFLMTLFNVRLGWWLGNPGVRGDHTF
                     YLASPRVAVRPIVAEAFGLTGSTGPYVYLSDGGHFENLGLYEMVLRRCHFIVVSDASC
                     DEGGVFQDLGSAVRKIRADMGVPIDFEGGIGILPRSADPAKRAASLYWALGRIRYSIA
                     DPSTAPGAAPGARDGWLLYVKPAFCGSEPPDVCEYAMAHDQFPHEPTTDQFFSESQFE
                     SYRMLGSHVMQQMSRDWTGGSLADFIAHVAARLPGGAGKAP"
BASE COUNT         1207 a         2642 c         2615 g         1190 t
ORIGIN      
        1 tggccacgag catatcgccg tcgaacggct tcggcagccc gactcctccc gccaccttgc
       61 gagccgactg gctagtctcc caggtcttga gccaacccgt ccccactgat ataactgccc
      121 cggccggccg cccccgtcgg gcctaaagga cgcggccaga tcggaggacc cgttggatcg
      181 acggacgttc gtctgcgcct tgactggcgg cctcctcgcc gcgccactcg ccgccgaggc
      241 gcagcagacg gggaagatgg ctcgcgtagg aattcttggc atcggtccgg ctccgagccc
      301 acaggagctg gcgaagtccg tctcgacaaa cccgttctgg ctgtcgatga gacacctcgg
      361 ctgggtcgag ggccaaaaca tggtcgtcga gcgccggttc ggcgagtcag ccgatcagct
      421 tcgcacagcc gccgctgacc tagtgcgcct caaggtcgac gtgctgtacg ttgcaagcgc
      481 gggcctggca aagatcctcc atctggaaac taaaaccatc cccatcgtgg ttggtcgcgc
      541 cgacagtgat ctcgttgcag gaggactcgt cgacagcctc gcaaggccgg ggggcaacat
      601 tacgggttca cagcttctta gcgatgatct cacccctaag cgcctggagc ttctcaaggc
      661 gcttgtaccg aatctttcaa gggccgcgct cctccaggag gacgtcaccg tctcggcagt
      721 cccccagacg cgcgcttggt ccgaacaagg ggccgctcgc ggggctatga cactcggcat
      781 agagctccat acgttcaccg tgcaccgacc ggcggacatc gctgccgctt tcctcggcat
      841 ggtgaagaat cgcgaccagg ccgtattggt gggggcgtcg ccctttgtgt tcgtgcatag
      901 aaaggccatc atcgatcttg ctgccgagca tcggatcgcc gctatctatt cgctccaagt
      961 gtatgttgag ccaggtggcc tcatgtccta tggagtaaat gttcccgaga tgcagcaacg
     1021 tgctgcggtc tatgtagaca aaatcttgaa gggcgccaag cctgccgacc tgccaatcca
     1081 gcagcccacg aagttcgacc tcgtgatcaa cctcaagacc gcgaaggccc tcggcctgac
     1141 gatcccggcg tcgctcttgc agcgggcgga tcaggtgatc gagtgataga ccggcgcacc
     1201 ttcctcggaa cagtgactgg cggcctcctc gccgcgccgc tcgtcgccga ggcgcagcag
     1261 gcggggaagg tgtaccggat cgggttcctc ggtaactcca ccgaggctct ggaggctaac
     1321 ctcgtcgggc cgttccgcga agggcttcgc cagcgcggct acgtcgaggg ccgggatctc
     1381 attatcgagt accgctgggc cgacggagcg tacgagcgct tcccggcgct catcgcggaa
     1441 ctcattgccc tcaaggtgga tatcatcgtc acggctggca cgcccgcggc cctcgcggtc
     1501 aagcgcacga ccacgacgat ccccctcatc atggtcgcgg taggcgaccc ggtcggcacg
     1561 ggcttggtca agagcctcgc gcgacctggc gggaacttga ccgggttggt ggcgatcgcg
     1621 ccggacctgg agggaaagcg cctggagctg ctcacgcaga tcgtacccaa gctctcgtcc
     1681 gtggccttcc tggggaatcc ggccaacccc atccacgtga cctcggaaaa gcaggccagc
     1741 gccgccgcca aggcgctgca cctgaaggtg gtgttctatc cggtgcgggt cgagggcgag
     1801 ttcgaccacg cgtttcaggc gatcgtcggc cagcggtccg gggcgctcgt catgctggcg
     1861 gatcggttct tcctgcatca ccgggcgcga atcgtcgagt tcaccgcccg gcaccggctc
     1921 ccggcggtgt acgcctacaa cgagctggtg caggtcggcg ggctgatgtc cttcggaccg
     1981 agctatcccg agatgcaccg gcgtgccgcc tactacgtgg acagaatcct caagggcgcg
     2041 aagcccggcg acctccccat cgagcagccg accaagttcg agctggtgat caacctcaag
     2101 accgccaagg ccctcggcct cacgatcccg ccgtcgctcc tgcagcgggc ggatcaggtg
     2161 atcgagtagc cagccactag ccagccgtgc gagccgtggc gtgtgaggcg atggttgcgc
     2221 tgctcctagc cggaaccctg tccgggtgtt cgatatcgcc tgaacggcaa gaggctctcc
     2281 gccgggcgtg ggcagaggga gaggccgaac aggcccaaga atgccagcgg catggcttcc
     2341 gatatacggc cggtgtctgt accggccccg gaggaggccc ctgatcaacg gggtagtacg
     2401 ccatcatgac agctcaactc cagcgcccat acctcgcgtc ctactgcatg atccaggcga
     2461 acggtacgat tctagtccag cgccgcttca acaccggcta tctggacggc cattgggcac
     2521 tcccttcggg gcatgccatt gacggagaag acgcgctaac ggccgcatcg cgcgaattgc
     2581 tcgaggaaac aaacctgatc gtgaaaccgg aggattggca attcgtttgc gccatgcatc
     2641 gtcgtaccga ccgaaccatc atcgacctgt tcttcacgac cgatacgttt gtcggcatcc
     2701 ctcgtatttg cgagccgacg aaatgcgacg gtcttgcatt ctttccgatg aatgcactgc
     2761 cccaaccgtt tgctggctat gtcggcgtgg cgctcgattg tctcagaggt atcagccctc
     2821 gccatggtcc ctattacagt gaaggctggc cgtaagcttg ctcggggtca tccaccgaca
     2881 gactactgcg cggtccatcc tccgtcgatg acgagctcgc tgcccgtcac gaacgcggcc
     2941 tcgtcggagg cgagatagag gacgccatag gcgacctcgt cgggctcgcc gtatcggccg
     3001 agcgggatgc gggacaccat gtactcgtag tggccgggat cgccgcggcg ggcctcggtc
     3061 atgggcgtca ccaccggtcc ggggtggacc gagttggccc ggatgcgctc ccgcgcgtac
     3121 tgcaccgcgg tgagcttggt gagcgtgcgc accgcgccct tggaggcctg gtactggggg
     3181 ctgctgcgct ccatgccgac catgccgagc tgggaggaga tgttgacgat ggagccgccc
     3241 ccggcgcggc gcattgcggg gatcgcggcc ttggtgccga ggaacacccc cttggcgttg
     3301 acgtccatgc cgtggtccca cgcctccgcg gtggtgtcct cgagccggct gcccccgccc
     3361 acccccgcgt tgttcaccag gacgtcgagc cgcccgaagc gggagaccgc ggtggccacc
     3421 gcctgctccc agtccgtctc gctcgtcacg tcgaggtgca cgtagaccgc ctgcccgccc
     3481 gctccggtga tcgcggcctc cacctgcttg ccctccgcgt cgaggagatc gccgaggacc
     3541 acccgcgcgc cctcgcgcgc gaagaggcgc gcctcggccg cgcccatgcc gcgggcgccg
     3601 ccgctgatca gcgcgacctt gcccgcgagc ctcatcgggt gccgaagagg cgatcgcccg
     3661 cgtcgccgag gccgggcacg atgtagccgt gctcgttcag gtggctatcg atcgcggcgg
     3721 tgtagaccgg cacgtcgggg tgcgcgcggt gaaacgcggc gaggccttcc gggcaagtca
     3781 gcaggcacac gaagcggatc gagcggggat gcgtggcccg cacgcgggtc accgccgcga
     3841 tcgccgagtt cccggtcgcg agcatcggat cgagcaccac cacgtcgcgc tccgccatct
     3901 cgtgcggcag cttgaagtaa tactcgaccg cctgcagggt ggccgcgtcg cggtagaggc
     3961 ccacgtggcc cacccgcgcg ctcggcagga tgcgcagcat gccgtcgagg aggccgttgc
     4021 ccgcgcgcag gatcgacacc agcacgatct tcttgccgtc gaggagccgg atggtggtgg
     4081 gcgccaccgg ggtctccgca gccacctcgt gggtgggcag gtcgcgggtg acctcgtagg
     4141 cgagcagcat gctgatctcg gagagcaggc tgcggaagac ggaggtgggc gtgtccttct
     4201 tccgcatgag cccgagcttg tgctgcacga gcggatggcg gatcacgacc acctcgcccg
     4261 tcgtcgacgg gcccgtccgc ggcgcctcgg tgtgcaccgc gggggactag accagctcga
     4321 gctgggcgcg gccccaggcc acgtcttccg cgcgcgcggg gctgaacgcg gccggggcct
     4381 cgtcgggcgc ggtgggcccc gcccccgcta ctgtggtccg gtgcatgacc cggcgatggc
     4441 gggactcgtc ccacggccgg ccgcggtgga gcatgcagcg gttgtcccac atcacgagat
     4501 cgcccacccg ccagcgatgg gtgtagacca gctcgggccg ggtggccgcc tcgcgcagct
     4561 cgcgcaggag cgcgcgcgcc tccgcggtgg gcatgcccac gatctcgcac gcgtgcgatc
     4621 ccacgtagaa cgcgcggcgt ccgttgaccg ggttggcccg cacgagcgcc tggcggaccg
     4681 gcggcacctg ggccgcgtgg tcgggcggca tcagcccgtc gtccaccagc ccgcgcgagt
     4741 agacgaagct gtgcaccgcg accttgtccg ccaggaacgc ctgcatgtcg acgggcagcc
     4801 gggcgtgggc cacccgcatg ctcgcgaact gggtctcccc gccctcgggc gggatctcgc
     4861 gggcggagag cagcgacgcg tgcgcgggca ccttcttgaa cgagctgtcg gtgtgccaca
     4921 tctggttgcc cgcgttgtag acgttgcgct tgtcgccggt cgggatcagc cggttctccc
     4981 cgtccacgtt ggagaggttg gaaatgtgga tgggagtgcc ctcccggctc gcgatgctgc
     5041 ggatggtggt ctcgagcggg ccgaagcgcc ggctgaacgc gatctgcgcc tcgtcggaga
     5101 gggactgccc gcggaagagc agcaccgagt gctcctcgaa ggcggccacg atctcggcga
     5161 aggtcgcgtc gtcctcgcgg gtgacgtcga gccctaccag ctccgcgccg aacagggggt
     5221 ggagcgctct ggtcgtcagt gacatggccc gccctcctct ctcagggtcc cgatcatatg
     5281 cgagcggtcg gggggcccgc aaccgcttgc gccgaccacc gcggcgggta tgctgctcgc
     5341 cgggaggctc ccccatgtcc accgtcgaat cgccggtcac gatcgtcgtc aaccgcctgg
     5401 ccctcggtcc caccgcgccg gacgcgggat cgccgctcct cacccgggtg ggcgaggtcg
     5461 tggcccagat gggcccgccg ccctggtcga agcggatcat cgcggacgag cggaatctgg
     5521 tgaccctcat cgcctcgccg cccggcggcg gcaaccggcc gcactggcac cgtgagttcg
     5581 acgagtggtg ggtggtgctg ggcgggcgcc tgcagtggga gctgaccggc gggatggtgg
     5641 tgaacgcggc caaggacgac atcgtgtggg tgccgcgagg caccgtgcac catatccgga
     5701 acgtgggcga cgaactctcg ctgcgcctgg cggtggccat gccgcccgcc tggcatatct
     5761 accacccgtg cgagcagtgc gggttcgcag acgacggtcc gcgcgagtgg tgcgcctgac
     5821 tcgactcgat cagggcgcct tgccggcccc cccgggcagc cgcgccgcga cgtgcgcgat
     5881 gaagtcggcg agcgagccgc ccgtccagtc gcggctcatc tgctgcatga cgtgggagcc
     5941 gagcatccgg tagctctcga actgcgactc gctgaagaac tggtcggtgg tgggctcgtg
     6001 cgggaactgg tcgtgagcca tcgcgtactc gcagacgtcg ggcggctcgc tgccgcagaa
     6061 cgcgggcttc acgtagagca gccagccgtc ccgcgcgccc ggagcggccc ccggcgcggt
     6121 cgacgggtcg gcgatcgagt agcggatgcg gccgagggcc cagtagaggc tggccgcccg
     6181 cttggccgga tccgccgagc ggggcaggat cccgatgccg ccctcgaagt cgatcggcac
     6241 gcccatgtcg gcgcggatct tccgcaccgc gctgcccagg tcctggaaga cgccaccctc
     6301 gtcgcagctc gcgtcgctga ccacgatgaa gtggcagcgc cgcagcacca tctcgtagag
     6361 cccgaggttc tcgaagtgcc cgccgtccga caggtagacg tagggtccgg tgctgccggt
     6421 gaggccgaag gcctcggcca cgatcgggcg caccgccacc cgtgggctcg cgaggtagaa
     6481 cgtgtggtca ccgcggacgc cggggtttcc gagccaccac ccgagccgca cgttgaagag
     6541 cgtcatcaga aaggtgacca gcggggagga gtgatagccc atgttggggc tcgcggccgc
     6601 gcccgagatc gtgacggcgg taccgagcga catgccctcc gtccccccgt agtgggcccg
     6661 cggcccgccc acgcccgcgc gggtgcggcg gtagcccacc atcaggctcc ccgcgtgcag
     6721 ggccgacgcg gtgaaggtgt gggccttgcg ctcctgccag gcgaggcgct cgccgtgcac
     6781 gagattgagc gccatgttca ccacgtgcag cagcctccgg cgcgggaccg ccgcgccggc
     6841 cggcgcgacc gacgagcgcg gccacagctc gtggagctgg aggttgtcgt tgtcgtcgaa
     6901 gccggtgaag gggttcgcgt gccggtcggt attcgacgcg cccaggtagg cccggatcag
     6961 ccggttgcgg tacatggcgt gcagcgagaa ccggttggtg ttgatgaacc aggccatcag
     7021 gagcccgagc gcggcgagcg ccatcgccac gccggccacg atccagatcg gtgacgatac
     7081 cagcaccccc acgtggtcga aggggccgtg cggccagttt ccccactgcg ggagtccgcc
     7141 cacgggatcg ccgctctgcc ggatgagcca gcccagcagc ccggagatca gggtggacag
     7201 cccgatcgcc agcaccgccg cgaacaccac gccggccagc ggcaccgcga gggccatcag
     7261 ccggtcgcgc gggctcgtct ggctcgacgc ccggtcacgt gcgccgagct tggtgaacca
     7321 tccgcccacc accgccatca gcccggacac tccgccgacc gcggtcaggg cccggctgat
     7381 ccagacatcg gagaacagca ccggcccgaa caggacgagc gcgccgaaca ccgtccacgc
     7441 cgagcccgcg atcaggaacc acgcgccgca gcgcgaccac cactcgcgat cgtcgtcctc
     7501 ggtccagcgg ctgagcaggc cgaccagcgc ggtcgcggcg aggaggaacg cgcccaccac
     7561 gaatccggga gccagcgcgg cgtagaggat cagggtgtag tccacccggc tgaacagctt
     7621 ggcagccagc agccacgcga tcgctccccc gacc
//
LOCUS       DATMCV010000473         4878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_160913, whole genome shotgun sequence.
ACCESSION   DATMCV010000473 DATMCV010000000
VERSION     DATMCV010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160913"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VNU03_10915"
     CDS             <1..220
                     /locus_tag="VNU03_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75020.1"
                     /translation="AQSLKMVDDLGGLEDAIEAAAKMAGLPAKPKVIYPRRRFSLREL
                     LRNELGLGPVSRILPSLETLRTPLYLMP"
     gene            369..644
                     /locus_tag="VNU03_10920"
     CDS             369..644
                     /locus_tag="VNU03_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HWP75021.1"
                     /translation="MTKAELIEEVAKVASLTKKETELIVNTVFDNITDALGKGDKVEL
                     RGFGSFRIRHRNSRKGRNPKTGDSVSVPEKRVPFFKVGKRLRELVNT"
     gene            649..1134
                     /locus_tag="VNU03_10925"
     CDS             649..1134
                     /locus_tag="VNU03_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWP75022.1"
                     /translation="MQQLWAPWRMAYVSTAGGPEAACVFCAALAVGDDRRALILKRGR
                     LAFLILNAFPYASGHLMAVITRHVGTLETATAEELAEAMTLLQSASRALGTAYHPDGF
                     NVGLNQGRVAGAGVLGHLHLHLVPRWNGDTNFMPVIGETRVLPEALEATYDRLAAALA
                     P"
     gene            1158..3722
                     /gene="mutS"
                     /locus_tag="VNU03_10930"
     CDS             1158..3722
                     /gene="mutS"
                     /locus_tag="VNU03_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWP75023.1"
                     /translation="MMRQYGELKRRFPDYLLLFRLGDFYELFNDDAHVASRLLQITLT
                     SRQNGVPMAGIPHHAADGYIARLIRAGQKVAVCEQLEEPAKGRKIVRRDVVRVITPGT
                     ITDTQFLDGARNNFLLAAHATSSAVGVALVDVSTGDFWVGEEPGAGDALLETALLRRP
                     AEILIAQSAEPGWVARWAPPGIPLTRVDGGAFGARAAREVLEAHFRGASLDGLGVGGL
                     TAGLQAAGAAVAYVRETQGDSLAHLTRVQRLVTGEAMLLDPTAVGTLELFETAQERTT
                     KGSLFATLDSTCTPMGARLLRQWMLRPLLDREGILRRQEAVGALVDAPAAREGLRQRL
                     GGVGDLERLTSRAALGVAHARDLIALRSFLGRLPGLAEDLGRLGTPLLATLAEEITPL
                     PDLQKLLEEALEDEPPLVLREGGLIREGWNDELRELKQAARQGKEWIASLEQRERART
                     GIPSLKVRFNRVFGYAIEVSNAHGARVPGDYLRRQTLVGAERYVTAELKEYESRVLGA
                     EERIARLEFELFGQVRARVAAEAAPLLRTARAVGTLDALVSLATVAHERGYVRPEVDD
                     GLALEIVDGRHPVLDAGGSPFTPNDTALDPASCQIMILTGPNMSGKSVFMRQVALLTL
                     MAQMGGFVPARSARVGLVDRILTRVGAQDNMARGQSTFLVEMVETASILHHATERSLI
                     LLDEVGRGTSTFDGLAIAWAVTEELHERGRGAKVLFATHYHELTALAERLPRVHNFHV
                     AVREWNDEIIFLHKVRPGGTDRSYGIQVARLAGLPPAVIARAKAILGELERERSGLEA
                     AVAPEPPAGEPTQLGLFPPTHDPLLRELAALDTGAMTPLQALNLLAEWQHRLKGRR"
     gene            3719..>4878
                     /gene="mutL"
                     /locus_tag="VNU03_10935"
     CDS             3719..>4878
                     /gene="mutL"
                     /locus_tag="VNU03_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWP75024.1"
                     /translation="MTAPRIRRLPDHLVNKIAAGEVVERPASVVKELVENALDALSTQ
                     VTVDLRDAGRALIRVTDDGVGMSTDEVDLALLRHATSKLATDADLDAITTLGFRGEAL
                     PAICAVTRFSVLSCPRGAETGTLVRGEGGTVSEKLLVAAPAGTTVEAQDLFFNTPARL
                     KFLKSAPAEVAAALRLLEATALAHLEVHFRVSHNGRPVLGAPRARTLRDRVGALWGFE
                     RAGRLLDVLRKDGPLAVSGLIAPPQLSRGSRDEIVLIVNGRPVRDTQLTQSLIEAYRP
                     LLSRDQFPVAVLHLELPPQEVDVNVHPTKAWVRFRAPRQVQEVLYRAVQDALRQFQVV
                     QTQRGLRTEGGSAVGATGDGVAAGSFFIESGGPEMSPALPQSADSPSGADDRA"
BASE COUNT          686 a         1825 c         1696 g          671 t
ORIGIN      
        1 ggcccagagc ctcaagatgg tcgacgatct gggcggcctg gaggacgcca tcgaggcggc
       61 cgccaagatg gcgggcctgc cggccaaacc caaagtcatc tacccgcggc gacggttctc
      121 gctgcgcgag ctgctgcgca acgagctggg actcggcccc gtctcgcgca tcctgccctc
      181 cctcgagacc ctccgtacac cgctctacct gatgccgtga ccggcccggg gtggggggtg
      241 ccggctaact acccagcctg cttgactaag tttcacctgc catggtacgg tcgcttccgt
      301 ggatcgggcc ggaccgccga tggtcacgac ggcccgggat cagtgtcggc ccttcgaagg
      361 gagtcgccat gacgaaagcg gagctgatcg aggaagtcgc gaaggtcgcg agcttgacca
      421 agaaggaaac cgagctcatc gtcaacaccg tgttcgacaa catcacggat gcgctcggca
      481 agggagacaa ggtcgagctg cggggcttcg ggagcttccg cattcgccat cgtaactccc
      541 gcaaggggcg caaccccaag accggtgaca gcgtgagcgt ccccgagaag cgcgtcccgt
      601 tcttcaaggt cggcaagcgc ctccgcgagc tcgtcaacac ctagtcgagt gcagcagctc
      661 tgggccccct ggcggatggc gtacgtctcg accgccggtg gccccgaggc cgcgtgcgtg
      721 ttctgcgccg cgctggccgt cggtgacgat cgacgcgcgc tcatcctcaa gcgcggccgc
      781 ctcgcgttcc tgatcctcaa cgccttcccc tacgcctccg ggcacctgat ggccgtcatc
      841 acccgccacg tcggcacgct cgagaccgcg accgcggagg agctggccga ggccatgacc
      901 ctcctccagt ccgcctcccg cgccctcggc accgcctacc atccggacgg cttcaacgtg
      961 ggattgaacc agggtcgcgt ggcgggggcg ggcgtgctcg gccacctgca cctgcacctg
     1021 gtcccgcgct ggaacggcga caccaacttc atgccggtga tcggcgagac gcgcgtgctg
     1081 cccgaggccc tcgaggcgac ctacgaccgc ctggccgccg cgctcgctcc gtgagggaag
     1141 ccgccgagct cacgcccatg atgcggcagt acggggagct gaagcgccgc ttccccgact
     1201 acctgctgct gtttcgcctg ggcgacttct acgagctgtt caacgacgac gcccacgtgg
     1261 cgtcccggct gctccagatc acgctgactt ctagacagaa tggggtcccg atggccggca
     1321 tcccgcacca cgccgccgac ggctacatcg cccgcctcat ccgcgccggc cagaaggtgg
     1381 cggtctgcga gcagctcgag gagccggcca agggccgcaa gatcgtgcgc cgcgacgtgg
     1441 tgcgggtcat caccccgggc acgatcaccg acacccagtt cctcgacggc gcccgcaaca
     1501 acttcctcct cgcggcacac gcgacgtcct ccgcggtggg cgtcgcgctg gtggacgtct
     1561 ccaccggcga cttctgggtc ggcgaggagc cgggagcggg cgacgcgctg ctggagaccg
     1621 cgctgctgcg ccgccccgcc gagatcctga tcgcccagag cgcggagccc gggtgggtgg
     1681 cccggtgggc gccgcccggc atcccgctga cccgggtgga cgggggcgcc ttcggggccc
     1741 gcgccgcgcg cgaggtgctc gaggcccact tccggggcgc gagcctcgac gggctcgggg
     1801 tgggcgggct caccgcgggg ctccaggccg ccggcgcggc ggtggcgtac gtgcgcgaga
     1861 cccagggcga ttcgctggcc catctcaccc gggtgcagcg cctggtcacc ggggaggcca
     1921 tgctgctcga cccgaccgcg gtgggcacgc tcgagctgtt cgagacggcc caggagcgga
     1981 ccaccaaggg ctcgctcttc gccaccctcg actccacctg cacccccatg ggcgcccggc
     2041 tgctccgcca gtggatgctg cggcccctgc tggaccggga gggcatcctc cgccgccagg
     2101 aggccgtggg ggccctcgtg gacgctcccg cagcccggga gggcctgcgg cagcggctgg
     2161 gcggggtggg ggacctcgag cggctcacca gtcgcgccgc cctcggggtg gcccacgcgc
     2221 gcgacttgat cgcgctgcgg agcttcctcg gccgtctgcc cgggctcgcg gaggatctgg
     2281 ggcggctcgg caccccgctc ctggcgaccc tcgcggagga gatcacgccc ctgcccgacc
     2341 ttcagaaact acttgaagaa gccctcgagg acgagccccc gctcgtcctg cgcgagggcg
     2401 ggctcatccg ggaggggtgg aacgacgagc tgcgcgagct caagcaggcc gcgcggcagg
     2461 gcaaggagtg gatcgcctcg ctcgagcagc gcgagcgggc gcgtaccggc atcccctcgc
     2521 tcaaggtccg cttcaaccgg gtcttcggtt acgccatcga ggtgagcaac gcgcacgggg
     2581 cgcgggtgcc cggcgactac ctgcggcgcc agaccctggt cggcgcggag cgctacgtca
     2641 ccgccgaact caaggagtac gagagccgcg tactcggcgc cgaggagcgc atcgcccggc
     2701 tcgagttcga gctcttcggc caggtgcgcg cccgggtcgc ggccgaggcc gccccgctcc
     2761 tccgcaccgc gcgggcggtc ggcaccctcg acgcgctggt ctcgctcgcc acggtcgccc
     2821 acgagcgcgg gtacgtccgc ccggaggtgg acgacggcct cgcgctcgag atcgtggacg
     2881 gccggcaccc ggtgctcgac gcgggcggct cgccgttcac gcccaacgac accgcgctcg
     2941 atcccgcgtc ctgccagatc atgatcctga ccggccccaa catgagcggc aagagcgtgt
     3001 tcatgcgcca ggtggcgctg ctcaccctca tggcccagat gggcggcttc gtccccgcgc
     3061 gctccgcccg cgtcgggctg gtcgaccgca tcctcacccg cgtcggcgcg caggacaaca
     3121 tggcccgcgg ccagagcacc ttcctggtgg agatggtcga gaccgcgagc atcctccacc
     3181 acgccacgga gcgcagcctc atccttctgg atgaagtcgg gcgcggcacc tccaccttcg
     3241 acgggctcgc catcgcgtgg gcggtgaccg aggagctgca cgagcgcggg cgcggcgcca
     3301 aggtgctctt cgcgacccac taccacgagc tgaccgcgct ggccgagcgg ctgccgcggg
     3361 tgcacaattt ccacgtggcg gtgcgcgagt ggaacgacga gatcatcttc ctgcacaagg
     3421 tgcggccggg cggcaccgac cgctcctacg gcatccaggt cgcgcggctc gccggcctgc
     3481 cccccgcggt gatcgcgcgg gccaaggcga tcctgggcga gctggagcgc gagcggagcg
     3541 ggctcgaggc cgcggtcgcc cccgagccgc ccgcgggtga gcccacccag ctcggcctct
     3601 tccctcccac tcacgatccg ctgctgcgcg agctcgccgc gctcgacacc ggggcgatga
     3661 cgccgctgca ggcgctcaac cttctcgcgg agtggcagca ccgactcaag gggcgccggt
     3721 gaccgccccg cgcatccgcc gcctgcccga tcacctcgtg aacaagatcg cggccggcga
     3781 ggtggtggag cggcccgcct cggtggtcaa ggagctggtg gagaacgcgc tcgacgcgct
     3841 cagcacccag gtgaccgtcg acctgcggga cgccggccgc gcgctgatcc gggtcaccga
     3901 cgacggggtc gggatgtcga ccgacgaggt cgacctggcc ctcctgcggc acgccacctc
     3961 caagctcgcc accgacgcgg atctcgacgc gatcaccacg ctcgggttcc gcggcgaggc
     4021 cctgcccgcg atctgcgcgg tcacccgctt ctcggtgctc tcgtgcccgc ggggcgccga
     4081 gaccggcacg ctcgtgcgcg gggaaggggg cacggtgagc gagaagctgc tggtcgcggc
     4141 gcccgcgggc accaccgtcg aggcgcagga tctcttcttc aatacgccgg cgcggctcaa
     4201 gttcctcaag agcgcgcccg ccgaggtggc cgccgccctg cggctgctcg aggccaccgc
     4261 gctcgcccac ctcgaggtgc acttccgcgt ctcgcacaac ggccgcccgg tcctcggcgc
     4321 gccccgcgcc cggaccctgc gcgaccgggt gggcgccctc tggggcttcg agcgcgcggg
     4381 ccgcctgctc gacgtcctgc gcaaggacgg accgctcgcg gtgtcggggc tgatcgcgcc
     4441 gccccagctc tcccgcggca gccgcgacga gatcgtgctg atcgtcaacg ggcgcccggt
     4501 gcgcgacacc cagctcaccc agagcctgat cgaggcctac cggccgctac tgtcgcgcga
     4561 tcagttcccg gtggcggtgc tccacctcga gctgccgccc caggaagtcg acgtcaacgt
     4621 ccaccccacc aaggcctggg tccgcttccg cgcgccccgc caggtgcagg aggtgctata
     4681 ccgcgcggtg caggacgcgc tgcgccagtt ccaggtggtg cagacacagc ggggactccg
     4741 gacggaaggt ggtagtgcgg tcggggctac cggggatggc gttgctgccg gaagcttttt
     4801 tattgaaagc gggggcccag aaatgtcccc cgcactcccc cagtccgcgg acagcccgtc
     4861 cggcgctgac gaccgtgc
//
LOCUS       DATMCV010000474         5188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_821312, whole genome shotgun sequence.
ACCESSION   DATMCV010000474 DATMCV010000000
VERSION     DATMCV010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821312"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VNU03_10940"
     CDS             <1..240
                     /locus_tag="VNU03_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWP75025.1"
                     /translation="TLKFARRMALMSPEALMATKLAINRGADAAGFRNAIRSGLDVLA
                     GLYAARTEVGTKFDEIREKEGLGAALRWRAEQFKE"
     gene            531..1118
                     /locus_tag="VNU03_10945"
     CDS             531..1118
                     /locus_tag="VNU03_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HWP75026.1"
                     /translation="MDPKVWQDMFALTLPVLEKILRPIIVYVFLVVGLRLAGKRELAQ
                     LNPFDLVVLLTLSNTVQNAIIGEDNSVSGGLIGATTLLAVNFVVVRFLYSHERLERVV
                     AGDSDVLIEDGLIRTDRLRHELITLPELEAAAHRQGFASLADIDRAILEPSGTICFVA
                     KTPTVEATRHQELVRRLEQIGQDLAAVRGALLARP"
     gene            1292..3760
                     /locus_tag="VNU03_10950"
     CDS             1292..3760
                     /locus_tag="VNU03_10950"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HWP75027.1"
                     /translation="MDAESVKRGILAHLEYTLAELPGHVDTEWEPYVALALAVRDRMI
                     ERWVRTQDTYYRVDPKRVYYLSLEYLMGRTLGNSLINLGLQEQCAKALQELGYRLEDL
                     REAEWDAGLGNGGLGRLAACFLDSMATLGYASYGYGLRYDYGIFHQRIVNGTQVEVPD
                     SWLRYGNPWEIPRVGDRFRVQFYGQVRTRLDAQGRIVKDWVDTRDVLATPFDTPIPGY
                     GTQTVNTLRLWGARAVQEFDLREFNEGEYIAAIQARAESENICRVLYPSDNVVAGQEL
                     RLAQEYFFVSATLQDIIRRYRKRYHLFDEPRGLRTFDRFADKVAIQLNDTHPALGVPE
                     LMRILVDVEGLAWDDAWTITQATFGYTNHTVLPEALERWPVGMIAAMLPRHLEIIYEI
                     NRRFLSEVTRRFGPDDARARRMSIIDEDGGRRVRMATLAIVGSHSVNGVAALHTEILK
                     RDVFREFHEMWPQRFNNKTNGITPRRWLLESNPPLAGLITEAIGPGWVTDLEALRGLG
                     ALAGDSAFAARWLAAKRENKRRLAETIARQYEHRGTTLTVNPDSLFDVQVKRIHEYKR
                     QLLNVLHVVTLYNRLKDGRGVDVPRTVIFGGKAAPSYTMAKLIIRLINAVGDTVNADS
                     AVRDRLAVAFLADYRVSLAEEIFPGAELSEQISTAGTEASGTGNMKFGLNGALTIGTM
                     DGATVEMAQEVGEDNIFIFGLTADEVAARRPHYDPREIYRTNGELARVLDMIAGGAFS
                     LGEPGLFRPLVDSLLGGDYFFLLADYASYVACQERVGQTYRDQPAWTRKSILNVAHMG
                     KFSSDRTIRQYADEIWGATPVPIE"
     gene            3798..4127
                     /locus_tag="VNU03_10955"
     CDS             3798..4127
                     /locus_tag="VNU03_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP75028.1"
                     /translation="MEFHGVDVGTQFKRMRELAPEAYRAFLEFDQKAFKDGAIPAKTK
                     ELIALGIAQITQCPWCIQAHTRKASEAGAADAEIAETTFVAMAMAAGAAWSHGGLALQ
                     CLQEHKG"
     gene            4180..4530
                     /locus_tag="VNU03_10960"
     CDS             4180..4530
                     /locus_tag="VNU03_10960"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWP75029.1"
                     /translation="MRYLLLIGSDNKNPGPPPPPAEMQAILQGHMKLGEALRAAGKVV
                     AAERLRPDSDGARVRAKAGHHQVMDGPFAETKEAIGGFYLIDCDTKAEAVEWAKKVPL
                     GDGGFVEVRPIWPM"
     gene            4594..>5188
                     /locus_tag="VNU03_10965"
     CDS             4594..>5188
                     /locus_tag="VNU03_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP75030.1"
                     /translation="MGSSDHPPPPEGHAHGPIAEVFRLEYGRLVASVARIVRDLDIAE
                     EIAQEAFERALDHWPTDGMPDRPGAWLLTTGRRRALDHLRRSRRAEGHAAALAYETTL
                     GAAIEGPETMDPEEIPDDRLRLIFTCCHPGLPADSRVALTLRLVGGLATPEIARAFLV
                     PEPTIAQRLVRAKRAIRERGLPYEVPEGPELADRLASV"
BASE COUNT          839 a         1762 c         1849 g          738 t
ORIGIN      
        1 accctgaagt tcgcgcgccg gatggcgctc atgtcgccgg aggccctcat ggcgaccaag
       61 ctcgcgatca accggggcgc cgacgcggcc ggcttccgca acgcgatccg gagcgggctc
      121 gacgtgctgg ccgggctcta cgcggcccgc accgaggtcg ggacgaagtt cgacgagatc
      181 cgcgagaagg aagggctcgg ggccgcgctc cgctggcggg cggagcagtt caaggagtag
      241 ccggcgcgct aggcccggca gcgctaggac gatcgccgcg gtcggcgctt ttcccccgcc
      301 cggagcgccc gccacaccag atctgacagg ctgagcgcct tggtgccggg gcccacgatc
      361 cgggccaccg ccagagcctt ccggcgtgag agaactcgaa ccggcggctt cctcctggcg
      421 gccatggccc gcgtattcta ttcgtcacac gggcgcaaag tccagtcggt gagactggtg
      481 gtccccacgg gaagcgagaa tgggggacgc gagaaatagg ggaggcgaga atggacccga
      541 aagtgtggca ggacatgttt gcgctcacgc tgccggtgct cgagaagatc ctgcggccga
      601 tcatcgtgta cgtgttcctg gtggtcgggc tgaggctcgc ggggaagcgg gagctggccc
      661 agctcaaccc gttcgatctc gtcgtgctgc tgacgctttc gaacacggtt cagaacgcga
      721 tcatcggcga ggacaattcg gtcagcggcg gattgatcgg cgcgaccacc ttgctggcgg
      781 tcaacttcgt cgtggtgcgg ttcctgtact cgcacgagcg cctggagcgg gtggtggccg
      841 gcgactccga cgtgctcatc gaggacggtc tgatcaggac cgaccgcctg cggcacgagc
      901 tcatcaccct gccggagctg gaggccgcgg cgcaccggca gggattcgcc tccctcgccg
      961 acatcgaccg cgcgatcctc gagccgagcg gcaccatctg cttcgtggcc aagaccccca
     1021 ccgtcgaggc gacgcggcat caggagctgg tgcgacggct cgagcagatc ggtcaggatc
     1081 tcgcggcggt gcggggggcg ctgctcgccc ggccgtaggc ccatcccggc gcgggtgatc
     1141 ccgatcggcg gtatcgtgtg atgggtagcc ggacggaacg gatcagatct ctctcgacat
     1201 ggaggtgccc atgacgctca acccgatcga ctcgcccacc cggatcgccg ccgcggcggc
     1261 gcgccaggcg ctcgcctacg acagcctcgg gatggacgcg gagtcggtga agcgcgggat
     1321 tctcgcgcac ctggagtaca cgctggccga gctgcccggc cacgtggaca ccgagtggga
     1381 gccctacgtg gcgctggcct tagcagtgcg ggaccggatg atcgagcggt gggtccgcac
     1441 ccaggacacc tactaccggg tcgaccccaa gcgggtgtac tacctctcgc tcgagtacct
     1501 gatgggccgc acgctgggaa acagcctgat caacctggga ttgcaggagc agtgcgcgaa
     1561 ggcgctgcag gagctcggct atcggctcga ggacctccgc gaagcggagt gggacgccgg
     1621 gctgggcaat ggcggcctcg ggcggctcgc cgcgtgcttt ctcgattcga tggcgacgct
     1681 cgggtacgcg tcgtacggct acgggctccg gtacgactac ggcatcttcc atcagcgcat
     1741 cgtgaacggg acccaggtgg aggttcccga cagctggctc cgctacggca atccctggga
     1801 gatcccgcgc gtgggcgacc ggttccgcgt gcagttctac ggccaggtcc gcacccggct
     1861 cgacgcgcag ggccgcatcg tgaaggactg ggtggatacc cgggacgtcc tcgccacgcc
     1921 cttcgacacg ccgatccccg gctacggcac gcaaaccgtc aacaccctcc gcctgtgggg
     1981 ggcgcgggcg gtccaggagt tcgatctccg cgagttcaac gagggcgagt acatcgcggc
     2041 gatccaggcc cgggccgaat cggagaacat ctgtcgggtg ctctacccga gcgacaacgt
     2101 ggtcgcgggg caggagctcc ggctggccca ggagtacttc ttcgtctccg ccacgctgca
     2161 ggacatcatc cggcgctacc ggaagcggta ccacctgttc gacgagcccc gcgggctccg
     2221 gacgttcgac cgcttcgcgg acaaggtggc catccagctg aacgacaccc accccgcgct
     2281 gggggtgccg gagctgatgc gcatcctggt cgacgtcgag ggactggcct gggacgacgc
     2341 gtggacgatc acccaggcca ccttcggcta cacgaatcac accgtgctgc cggaggcgct
     2401 ggagcgctgg ccggtcggca tgatcgcggc gatgctcccc cggcacctcg agatcatcta
     2461 cgagatcaac cggcgcttcc tgagcgaggt gacccgtcgg ttcgggcccg acgacgcgcg
     2521 cgcccggcgg atgtcgatca tcgacgagga cggggggcgg cgggtgcgga tggccaccct
     2581 cgccatcgtc ggcagccaca gcgtcaacgg ggtggccgcg ctgcataccg agatcctcaa
     2641 gcgcgacgtc tttcgcgagt tccacgagat gtggccgcag cggttcaaca acaagaccaa
     2701 cgggatcacc ccgcgccgat ggctgctcga gagcaatccg ccgctggccg ggctgatcac
     2761 cgaggcgatc ggccccggct gggtgaccga cctggaggcg ctccgcgggc tgggcgcgct
     2821 ggcgggcgac tcggccttcg cggcccgatg gctcgccgcc aagcgggaga acaagcgccg
     2881 cctggccgag acgatcgccc gccagtacga gcaccgcggg acgacgctca ccgtgaaccc
     2941 cgactcgctc ttcgacgtgc aggtcaagcg gatccacgag tacaagcgcc agctcctgaa
     3001 cgtgctgcac gtcgtcacgc tctacaaccg cctcaaggac gggcgcgggg tcgacgtgcc
     3061 gcggaccgtc atcttcgggg gcaaggcggc gccgagctac accatggcca agctcatcat
     3121 ccggctcatc aatgcggtcg gcgacaccgt gaacgcggac tccgccgtcc gcgaccggct
     3181 ggccgtcgcg ttcctggccg actaccgggt gtcgctcgcc gaggagatct ttcccggggc
     3241 cgagctgtcc gagcagatct cgaccgcggg gaccgaggcg tcggggaccg gcaacatgaa
     3301 gttcgggctg aacggggccc tgaccatcgg caccatggac ggggccaccg tggagatggc
     3361 ccaggaggtg ggggaggaca acatcttcat cttcggcctg accgcggacg aggtcgcggc
     3421 ccggcgaccg cactacgatc cccgggagat ctaccgcacg aacggcgagc tggcccgcgt
     3481 gctggacatg atcgcgggcg gggcgttcag cctgggggag ccgggcctct tccggccgct
     3541 ggtggactcg ctcctgggcg gggactactt cttcttgctc gcggactacg cgtcctacgt
     3601 cgcctgccag gagcgcgtcg ggcagaccta tcgcgatcag ccggcgtgga cgcggaagtc
     3661 gatcctgaac gtggcccaca tgggcaaatt ctccagcgac cggacgatcc ggcagtacgc
     3721 cgacgagatc tggggcgcca cgccggtccc catcgagtaa gtccagcagg ggcagtaagc
     3781 ccgacaagga ggcagccatg gagttccacg gagtcgacgt cggcacccag ttcaagcgca
     3841 tgcgggagct ggccccggag gcctaccgag ccttcctcga gttcgatcag aaggccttca
     3901 aggacggggc gatccccgcc aagacgaagg agctcatcgc gctcggcatc gcccagatca
     3961 cccagtgccc ctggtgcatc caggcccaca cccgcaaggc atcggaggcc ggcgccgcgg
     4021 acgcggagat cgcggagacc accttcgtgg cgatggcgat ggcggccggc gcggcctgga
     4081 gccacggggg gctcgcgctc cagtgcctcc aggagcacaa gggatagcgt gtcgatttcg
     4141 atgggtcatc gacgtcgtat gggatgagag gagaccccaa tgcgctactt gctactgatc
     4201 ggctcggaca acaagaaccc agggcccccg ccacccccgg cggagatgca ggcgatcctc
     4261 cagggccaca tgaagctcgg cgaggcgctg cgcgcggccg gcaaggtcgt cgcggcggag
     4321 cggctgcgcc cggacagcga cggggcccgc gtccgcgcca aggcgggtca ccatcaggta
     4381 atggacgggc ccttcgccga gaccaaggag gcgatcggcg gattctacct gatcgactgc
     4441 gacaccaagg cggaggcggt cgagtgggcc aagaaggtgc cgctcgggga tggcggcttc
     4501 gtcgaggtgc ggccgatctg gccgatgtag gcaggccggc gcccgcgtcg gcccgcgcgc
     4561 gggggacggc gggatcgcgt aacatcccgg ttcgtgggat cgagcgatca cccgcccccg
     4621 ccggagggcc acgcccatgg gccgatcgcg gaggtgttcc gcctggagta cgggcggctg
     4681 gtcgcctccg tggcccggat cgtgcgcgat ctcgacatcg cggaggagat cgcccaggag
     4741 gccttcgagc gtgcgctcga ccactggccg accgacggga tgcccgatcg cccgggcgcc
     4801 tggcttctca ccaccgggcg acggcgcgcg ctggaccacc tgcggcgcag ccggcgggcc
     4861 gagggccacg cggcggccct ggcctatgag acgacgctcg gcgcggcgat cgagggcccc
     4921 gagacgatgg atcccgagga gatcccggac gaccggctcc ggctgatctt cacctgctgc
     4981 cacccgggcc tgcccgcgga cagccgggtc gcgctcaccc tgcggctggt gggcggtctc
     5041 gcgacgcccg agatcgcccg ggccttcctg gtgcccgagc cgaccatcgc gcagcgtctc
     5101 gtccgcgcca agcgcgcgat tcgcgagcgg ggcctgccct acgaggtgcc ggaagggccc
     5161 gagctggccg atcgcctggc ttccgtgc
//
LOCUS       DATMCV010000475         7074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_359840, whole genome shotgun sequence.
ACCESSION   DATMCV010000475 DATMCV010000000
VERSION     DATMCV010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359840"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..982
                     /locus_tag="VNU03_10970"
     CDS             62..982
                     /locus_tag="VNU03_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWP75031.1"
                     /translation="MTPEERRYYATQSRVSDPADRGTLLAALPAEPAGLVPAISGLVL
                     HTLFVRPLGITAPAGSEADVESRSVPRILDRILERDAAPLDVARPPERRFIGICRDYA
                     LLACAALRQHRVPARLRVGFAAYFTPGYLEDHWVCEYHAGDRWRLLDPELSPRVRSHF
                     GIAFDPADVPREGFLVAGEVWRRVRAGAIDPAICGVSSIGVGGHGFIAASVARDLAAL
                     NKREMLAWDVWGLPLGLRVGGPVPPDRPVPEDTARRLDAVAALTATPEPDWGRLREAH
                     DRDDGFRVPSVVTSFTSRGPAQVTVDVDLS"
     gene            1046..2194
                     /locus_tag="VNU03_10975"
     CDS             1046..2194
                     /locus_tag="VNU03_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75032.1"
                     /translation="MLERLISGDNHIDLTYCPPDLWSSQAPSKWKLLVPRVEELEDGC
                     HWFVEAQDKGMWNGVGPGFLKRTPGTFARIDEMASCGFEWDHRAGARPRPTTPELRLA
                     DLDRDGLDAEVVYGCLMINDMIASGERRGWANAIYNDWAADFAKKSDPNRIYPLAIVP
                     NHDPQAAAAEVRRCAKMGLKGGDLGFKSMSIPLYQHDWYPLWEAAAECHFPIAFHSTG
                     FKAVRAPDSPALEQQYMLQWRLVRSSLFQLDTMEVLVSIITSGACEKYPDFKFVLGES
                     GVTWLPYVFDRFDTEYEDRARGLGFSMKPSDYFKRQGYVTYQQDQFLEPIIPLVGEDN
                     IIWGADYPHPDCVWPDSRRHLEKNLGNLPERIQRKITYDNVVKLYNLR"
     gene            2208..2987
                     /locus_tag="VNU03_10980"
     CDS             2208..2987
                     /locus_tag="VNU03_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWP75033.1"
                     /translation="MTSTALEIPSLKGKVSEEEWAVRVDLAAAYRLVSRYGWEDLVFT
                     HISARVPGTEDQFLINPYGLFFDEITASSLVKIDQEGNKLGDSPFNVNAAGFIIHSAI
                     HAVRHDAKCVLHTHTANGVAVSTQRAGLLPISQHSLFILNSVGYHDFEGPALDADEKP
                     RLQADLGDNRCLILRNHGLLTVGETVADAFVNMYYLEASCQIQVRAQSGGGELISVPK
                     EVMDKGYANAVANQRRNGGQGRLVWPGLLRRLDRTDSSFRN"
     gene            2998..5370
                     /locus_tag="VNU03_10985"
     CDS             2998..5370
                     /locus_tag="VNU03_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWP75034.1"
                     /translation="MTQPESSLRFGVGAGARRSEDGPLLTGGGRFTDDLSVPDQAHAV
                     FVRATVAHGTIRRVDVASALTLPGVLAILTGADARAAGLGAIPPAASFPGRGGTPLAS
                     APIPPLAIDRVRYVGEAVAIAIAETPEQAADAAAAVGVDLDPLPGAADVETALAHGAP
                     TIWPDAPGNVAFDWADGDAATVDAAFAGAAHVARVRLHDTRLAPAAMEPRAAIGRWDP
                     ADGRYTLIASTQGVAVVRRLLAEGVLKIPVQQLRVLTPDVGGGFGMKVQAYPEYAALL
                     FAARRVGRPVKWRATRVESFLADTAGRDGMLEAELALDAGGRFLGLRMRTRVGLGAYV
                     TAFAAVFGTNNTKNCLSSVYRIPVIGIDVTLAFTNTAPLGPYRGAGRPEALYVIERLI
                     DQAAIALGVDRVELRRRNLIPPAAMPYRTPNGPIYDSGEFETVMDRALELADWTGFPA
                     RRAASERAGRLRGIGLGCFLEVAGGILDETVDLRFGPDGTVALHTGAQPMGQGHLTTF
                     PPVIAARLGIDPSAVRLVQGDSDLVPAGTPSVASRSMMMAGSAAALACDGAVDKGRRV
                     AAHYFEAAAPDVEFAAGHFRVRGTDRAIGLLDLAARARALPGLPADLADGLDTTAKFV
                     SPQMSFPNGCHVTEVEIDPETGRVAVVAYAAVDDVGRIVHETIVEGQIHGGIAQGLGQ
                     VLGEHVVYDEHGQLLTGSFMDYMLPRADDLPSLRVGHHGVPCTTNPLGVKGAGESGVA
                     GALPSAVNAVLDALGTRGVHELDLPMSPARVWAALQASTPGDIDRSRRAR"
     gene            5380..5967
                     /locus_tag="VNU03_10990"
     CDS             5380..5967
                     /locus_tag="VNU03_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWP75035.1"
                     /translation="MAEPDVRTRRWTRIEYDRLIDAGFFRPGNPVELLGGQMVVSEPQ
                     GSRHFATLRATQEALREAFGDRWEIRGQGPIALDDESEPEPDLAVVPGTFRDYRAAHP
                     SSPSLVVEVSETSLALDRAHKGSLYARAGLVDYWIVNLPERRLEVYRDPEPDETAPFG
                     WRYRSVTVLEPGLEPGASVSPLARPDLRVKISDLF"
     gene            complement(5964..6587)
                     /locus_tag="VNU03_10995"
     CDS             complement(5964..6587)
                     /locus_tag="VNU03_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWP75036.1"
                     /translation="MTSRRGPGTVRSFPARAALGFVTLALLTSAAAAHAQTVDERFTV
                     CLACHGADGQSRIPETPSLGGQPSFFVVAQLFLFREGRRDNAAMIAAAKGLTNDDLLA
                     FAERVTKLPPPPPPEDPPDAARFARGRTLTLRHPCGVCHNPDFSGREQMPRLANQREE
                     YLLKAMREYKSGARLGYGGAMSQELAGLSDQDLIDLAHFLAHQGAKP"
     gene            complement(6584..>7074)
                     /locus_tag="VNU03_11000"
     CDS             complement(6584..>7074)
                     /locus_tag="VNU03_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWP75037.1"
                     /translation="YFTDNQRDWLSEDMPNDELNRVTKLGQHFGYPYCHQGDFADPQF
                     GWGKACEDFVKPVLLLGPHSAPLGMRFYTGKMFPPKYQNAIFIARHGPWNRTKKYAAD
                     VVAVFLNPNGSVKSMEPFLTGLVENNGYLGRPVDVLVMKDGSLLVSDDHAGAVYRISY
                     KK"
BASE COUNT          991 a         2624 c         2441 g         1018 t
ORIGIN      
        1 gacaagacct acctgacgat cctctccggt cgccggtgag cgcgacccgg tgactccgcc
       61 catgaccccc gaggagcgcc ggtactacgc gacccagagc cgggtttccg atccggcgga
      121 tcggggcacg ctcctcgccg ccctgcccgc cgagcccgcg ggcctcgtcc ccgcgatctc
      181 cggcctcgtc ctgcatacgc tcttcgtgcg cccactcggc atcaccgcgc ccgcgggcag
      241 cgaggccgac gtcgagagcc ggagcgtgcc gcgaatcctc gaccgcatcc tggagcggga
      301 cgccgcgccc ctcgacgtgg cgcgcccgcc cgagcgccgc ttcatcggca tctgccgcga
      361 ctacgccctg ctggcgtgcg ccgcgctccg ccagcaccgc gtcccggcgc gcctccgcgt
      421 cggcttcgcc gcctacttca cgccgggcta tctcgaggat cactgggtgt gcgagtacca
      481 cgccggcgac cgctggcggc tcctcgatcc cgagctgtcc ccccgcgtac ggtcccactt
      541 cgggatcgcg ttcgatccgg ccgacgtgcc ccgcgagggg ttcctggtgg ccggggaggt
      601 ctggcgccgc gtgcgcgccg gcgcgatcga cccggcgatc tgcggggtct ccagcatcgg
      661 ggtcggcggc cacgggttca tcgcggcgag cgtggcccgt gacctggccg ccctcaacaa
      721 gcgcgagatg ctggcctggg acgtctgggg gcttccgctc gggctccgcg ttggcgggcc
      781 ggtaccgccc gatcggccgg tacccgaaga caccgcccgc cgcctggacg cggtggccgc
      841 gctgaccgcc acgcccgagc ccgactgggg gcgtctgagg gaggctcacg accgcgacga
      901 tggcttccgg gtgccctcgg tcgtcaccag cttcacctcg cgcgggccgg cccaggtcac
      961 ggtagacgta gacttgtcct agccgccgct ctgggggtac gatcgcgcca cgaggagctt
     1021 cccccgtacc gccaaggagg gcgtcatgct cgagcggctc atctccggcg acaaccacat
     1081 cgacctcacc tattgcccgc ccgacctctg gtccagtcag gcgccctcca agtggaagct
     1141 cctggtgcca cgcgtggagg agctggaaga cggctgccac tggttcgtgg aggcgcagga
     1201 caaggggatg tggaacgggg tgggccccgg cttcctcaag cggaccccgg gcaccttcgc
     1261 gcggatcgac gagatggcct cctgcgggtt cgagtgggac caccgggcgg gagccaggcc
     1321 ccggccgacc acccccgagc tgcgcctggc cgacctcgac cgtgacggcc tcgacgccga
     1381 ggtggtgtac ggctgcctga tgatcaacga catgatcgcg agcggcgagc gccgcggctg
     1441 ggccaacgcg atatacaacg actgggccgc cgacttcgcg aagaagtcgg accccaaccg
     1501 gatctacccg ctggcgatcg tgcccaacca cgatccccag gccgccgcgg ccgaggtgcg
     1561 ccgctgcgcc aagatgggcc tcaagggcgg cgacctcggc ttcaagagca tgagcatccc
     1621 gctctaccag cacgactggt acccgctctg ggaggccgcg gccgagtgcc acttcccgat
     1681 cgcgttccac tcgaccggct tcaaggcggt gcgcgccccc gactcccccg cgctggagca
     1741 gcagtacatg ctccagtggc ggctggtgcg ctcctcgctc ttccagctcg acaccatgga
     1801 ggtgctggtc tcgatcatca cctccggggc gtgcgagaag tatcccgact tcaagttcgt
     1861 cctgggcgag tcgggcgtga cctggctccc ctacgtgttc gatcgcttcg acaccgagta
     1921 cgaggaccgc gcccggggcc tcggcttctc catgaagccg agcgattact tcaagcgcca
     1981 gggctacgtg acctaccagc aggatcagtt cctcgagccg atcatcccgc tggtgggcga
     2041 ggacaacatc atctggggcg cggactaccc gcatcccgac tgcgtgtggc cggactcgcg
     2101 ccgccacctc gagaagaacc tcggcaacct gccggagcgc atccagcgca agatcaccta
     2161 cgacaacgtg gtcaagctct acaacctccg ctgagcgaaa ggaccccatg accagcaccg
     2221 cgctcgagat tccgtcgctc aagggaaagg tatctgagga ggagtgggcc gtccgggtgg
     2281 acctggccgc cgcctaccgg ctggtgtcgc gctacggctg ggaggacctg gtcttcaccc
     2341 acatctcggc ccgcgtcccg ggcaccgagg accagttcct gatcaacccc tacggcctct
     2401 tcttcgacga gatcaccgcg tcgagcctgg tcaagatcga ccaggagggc aacaagctcg
     2461 gggactcgcc gttcaacgtc aacgcggccg gcttcatcat ccacagcgcg atccacgccg
     2521 tccgccacga cgccaagtgc gtgctgcaca cccacaccgc caacggggtc gcggtctcca
     2581 cccagcgggc cggcctcctg ccgatctcgc agcactcgct gttcatcctc aacagcgtgg
     2641 gctaccacga cttcgagggc cccgcgctcg acgcggacga gaagccgcgc ctgcaggcgg
     2701 acctgggcga caatcgctgc ctgatcctcc gcaaccacgg tctgctcacc gtcggcgaga
     2761 cggtggcgga cgccttcgtg aacatgtact acctcgaggc ctcctgccag atccaggtgc
     2821 gcgcccagtc gggcggcggc gagctgatct cggtgcccaa ggaagtcatg gacaagggct
     2881 acgccaacgc ggtggccaat cagcgccgca atggcggcca gggccggctc gtgtggccgg
     2941 gcctcctgcg ccgcctggat cgtacggact cgtccttcag gaactgatcg gccggaattg
     3001 acccagcccg agtcctcgct gcgcttcggc gtcggagccg gcgcgcggcg cagcgaggac
     3061 gggccgctcc tcaccggcgg cggccgcttc accgacgacc tctcggttcc cgaccaggcc
     3121 cacgccgtct tcgtgcgcgc gacggtcgcc cacgggacga tccggcgcgt ggacgtcgcg
     3181 agcgcgctca ccctgcccgg tgtcctcgcg atcctgaccg gggccgacgc gcgggcggcc
     3241 ggcctcgggg ccatcccacc ggccgcctcc ttccccggcc gcggcggcac accgctcgcc
     3301 tcggcgccca tccccccgct ggcgatcgac cgcgtccgct atgtaggcga ggcggtcgcc
     3361 atcgcgatcg cggagacccc ggagcaggcc gcggatgcgg cggccgcggt cggggtcgat
     3421 ctcgacccgc tccccggcgc ggccgacgtg gagaccgcgc tcgctcacgg cgcgcccacg
     3481 atctggcccg acgctccggg caacgtggcg ttcgactggg ccgacggcga cgcggccacg
     3541 gtggacgcgg ccttcgcggg agccgcgcac gtcgcgcgcg tccggctgca cgacacgcgc
     3601 ctggcccccg ccgccatgga gccgcgcgcg gccatcggcc gctgggaccc ggccgacggg
     3661 cggtacaccc tcatcgccag cacccagggc gtcgcggtcg tccgccgcct cctcgcggag
     3721 ggcgtgctca agatccccgt ccagcagctc cgggtgctca cccccgacgt gggcggcggc
     3781 ttcggcatga aggtccaggc ctacccggag tacgcggccc tcctcttcgc ggcccgccgg
     3841 gtgggccggc cggtgaagtg gcgcgccacg cgcgtggaga gcttcctggc cgacaccgcg
     3901 ggccgcgacg gcatgctcga ggcggagctg gcgctggacg cgggcgggcg cttcctgggg
     3961 ctccgcatgc gcacccgcgt gggcctcggc gcctacgtca ccgcgttcgc ggcggtgttc
     4021 ggcaccaaca acaccaagaa ctgcctctcg agcgtctacc gcatcccggt catcggcatc
     4081 gacgtgaccc tcgccttcac caacacggcg ccgctcgggc cctatcgcgg cgcggggcgg
     4141 ccggaggcgc tctacgtgat cgagcggctg atcgaccagg ccgcgatcgc gctcggcgtc
     4201 gaccgcgtcg agctgcgccg gcgcaacctg atccctcccg ccgccatgcc ctaccgcacg
     4261 cccaacgggc cgatctacga cagcggcgag ttcgagaccg tgatggaccg ggcgctcgag
     4321 ctggccgact ggacgggctt cccggcccgc cgggccgcct cggagcgcgc ggggcggctg
     4381 cgcggcatcg ggctcggctg cttcctcgag gtggcgggcg gcatcctgga cgagacggtg
     4441 gatctgcgct tcggccccga cgggaccgtc gcgctccaca ccggcgccca gccgatgggc
     4501 cagggccacc tcacgacttt cccccccgtg atcgcggcgc ggctcggcat cgatccgtcc
     4561 gcggtgcgcc tcgtccaggg cgacagcgac ctggtcccgg ccggcacccc gagcgtggcg
     4621 tcgcgctcca tgatgatggc gggcagcgcg gccgcgctcg cctgcgacgg cgcggtcgac
     4681 aaagggcgcc gcgtggcggc gcactacttc gaggccgccg cgcccgacgt ggagttcgcg
     4741 gccgggcatt ttcgcgtccg cggcaccgac cgcgcgatcg gcctgctgga cctggccgcc
     4801 cgcgcccgcg cgctgcccgg gctgcccgcg gatctggccg acggcctcga caccaccgcc
     4861 aagttcgtct cgccccagat gagcttcccc aacggctgcc acgtcaccga ggtcgagatc
     4921 gacccggaga ccggccgggt ggcggtggtc gcctacgccg cggtcgacga cgtggggcgc
     4981 atcgtgcacg agaccatcgt ggaggggcag atccacggcg gcatcgccca ggggctgggc
     5041 caggtgctgg gcgagcacgt cgtctacgac gagcacggcc agctcctcac cgggtccttc
     5101 atggactaca tgctgccgcg cgccgacgac ttgccgtcgc tgcgggtggg ccaccacggc
     5161 gtcccctgca ccaccaaccc gctcggggtg aagggcgcgg gcgagtcggg cgtggccggc
     5221 gcgctgccct cggcggtgaa cgcggtgctc gacgccctcg gcacgcgcgg cgtgcacgag
     5281 ctcgacctgc cgatgtctcc ggcgcgagtc tgggccgcgc tccaggcgtc cacgccagga
     5341 gacattgacc ggtcgcgacg ggctcgctag gctctttgca tggctgagcc ggacgtacgg
     5401 acgcgacgct ggacgcgcat cgagtacgat cgcctgatcg acgccggctt ctttcgcccg
     5461 ggaaatcccg tcgagctcct cgggggccag atggtcgtct ccgagccgca gggcagccgc
     5521 cacttcgcga ccctgcgcgc cacccaggag gcattgcggg aggccttcgg cgaccggtgg
     5581 gagattcgcg gccaggggcc gatcgcgctc gacgatgagt ccgagcccga gccggatctg
     5641 gcggtcgtgc ccggtacctt tcgcgactac cgggcggctc acccgtccag cccgtcactc
     5701 gtggtcgagg tcagcgagac cagcctggcc ctggaccgcg cccacaaggg cagcctctat
     5761 gcccgggccg ggctcgtcga ctactggatc gtgaacctgc ccgagcgtcg actcgaggtc
     5821 tatcgggacc ccgagcccga cgagaccgcc ccattcggct ggcgataccg ctcggtcacc
     5881 gttctggagc caggcctgga gccgggcgcc tccgtgtcac cgctggcccg acctgatctg
     5941 cgcgtcaaga tctccgatct cttctagggc tttgccccct ggtgggcgag gaagtgggcg
     6001 aggtcgatca ggtcctggtc ggagagcccg gccagctcct gcgacatggc cccaccgtag
     6061 ccgagccggg cgccgctctt gtactcccgc atggccttga gcaggtactc ctcccgctga
     6121 ttggccaggc gcggcatctg ctcgcggccg gagaaatccg ggttgtggca gaccccgcac
     6181 gggtgccgca gggtcagcgt ccgcccccgc gcgaagcggg cggcatcggg cgggtcctcc
     6241 ggaggcgggg gcgggggcag cttggtgacc cgctcggcaa aagccaggag gtcgtcgttg
     6301 gtgagcccct tcgccgccgc gatcatggcg gcgttgtccc gccggccctc acggaacagg
     6361 aagagctggg cgaccacgaa gaacgagggc tgccccccga gggacggcgt ctcggggatc
     6421 cgggactggc cgtcggcgcc gtggcaggcc aggcagaccg tgaaccgctc gtccacggtc
     6481 tgcgcgtggg ccgccgcggc cgaggtgagg agcgccagcg tgacgaagcc cagcgcggcg
     6541 cgggccggga acgatcgtac cgtccccggc ccgcggcgag acgtcacttc ttgtagctga
     6601 tccggtagac cgcgcccgcg tggtcgtcgg agacgagcag cgagccgtcc ttcatgacga
     6661 gcacgtcgac cgggcgcccg agatagccgt tgttctcgac cagcccggtg aggaagggct
     6721 ccatcgactt cacgctcccg ttggggttca ggaacaccgc caccacgtcg gcggcgtact
     6781 tcttggtgcg gttccacggc ccgtggcggg cgatgaagat ggcgttctgg tacttcggcg
     6841 ggaacatctt gccggtgtag aagcgcatgc cgagcggggc cgagtgcggc cccagcagca
     6901 ggaccggctt cacgaagtcc tcgcaggcct tgccccagcc gaactgcgga tccgcgaagt
     6961 cgccctggtg gcagtacgga tagccgaagt gctggccgag cttggtgacc cggttcagct
     7021 cgtcgttcgg catgtcctcg gagagccagt cacgctggtt gtcggtgaag taga
//
LOCUS       DATMCV010000476         3032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_22911, whole genome shotgun sequence.
ACCESSION   DATMCV010000476 DATMCV010000000
VERSION     DATMCV010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22911"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VNU03_11005"
     CDS             complement(<1..116)
                     /locus_tag="VNU03_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HWP75038.1"
                     /translation="MPSQPSKTLEVVPNPHPDRDYEVSLEIPEFTCICPRTG"
     gene            complement(167..1081)
                     /locus_tag="VNU03_11010"
     CDS             complement(167..1081)
                     /locus_tag="VNU03_11010"
                     /inference="COORDINATES: protein motif:HMM:NF017807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWP75039.1"
                     /translation="MPLRPLDWRGASTAIFLAVLWGGNPVAVKIGLVDAPPLRLALFR
                     FVLGGLTILAYAWWTRHRGIFDLRPGEWRVLISLGVIFSVQIGTMNVGLGLTTAAHGA
                     VLLNSYAVHTVVLAHFLIPGDRLTPAKVGGVLVAYTGIVLLFARDFSFQSSTLLGDVV
                     VMGSAVLLGERVVYMARAVQRLDPIKLLVFQSAIGSACFLIASLVFEGATPTRYTWGL
                     ATSLFYQGAVIAGFNFVVNSYLFQVYLASALATCSLTTPLAGVLIAAAVTGDRLSPLL
                     LLSSVMVAAGIGLTVRRPPLLPPGERAG"
     gene            1144..2223
                     /gene="ychF"
                     /locus_tag="VNU03_11015"
     CDS             1144..2223
                     /gene="ychF"
                     /locus_tag="VNU03_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWP75040.1"
                     /translation="MKIGLIGLPKSGKTSLFNLLTGATVATSSFGTARGEMHAGVARV
                     PDARVDRLVTLFKPKKITLATFEVVDLAGIAKGERQGLEAKEFRNADALLHVVRAFPD
                     TGGAAPDPAGDIDDLDTELILADLEVVERRLERLEAQIKKKRTDADVHEQAVLIRMKP
                     DLEAGRPIRAMGLSDDETRAIRGFTFLSQKPILHCLNLAEKEIARGKTVVESFGLAEV
                     AARPGTRAGWVSAVIESEVAQLEGSEQAAFLADLGLTEPAIRRVLEDCYTLLGLISFF
                     TVGEDEVRAWSIPRNTRAQDAASAIHSDIARGFIRAEVVGYDDLMALEGSMSAARDRG
                     RLRLEGKDYQVQDGEICHFRFNVAK"
     gene            complement(2291..2866)
                     /locus_tag="VNU03_11020"
     CDS             complement(2291..2866)
                     /locus_tag="VNU03_11020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWP75041.1"
                     /translation="MGPVPVMLGAMLLLAPVGAARADDAPDVTILSAYRSLAGANELP
                     RLRPHAGVDIAVPVGTPVLAAADGTVSLLIDYEQGCGNGVVLAHPAFSRWTVYCHLTR
                     AVVATGQVVARGQVIGFSGTSGNSANVPHVHLELCTVACASHRDGDLRGTQDPIPLVV
                     GCFEAERAYPTDRLALTWPLGCRRGTADARR"
BASE COUNT          495 a         1042 c         1129 g          366 t
ORIGIN      
        1 tgcccggtgc gcgggcagat gcaggtgaac tcggggatct cgaggctcac ctcgtagtcg
       61 cggtcggggt gcgggttcgg gacgacctcg agggtcttcg acggctggct gggcatgccg
      121 cgacgctacc gcgggggtgg agaccggtca aggtggagcc gacccctcac cctgccctct
      181 ccccaggggg gagaagggga ggtcggcgga cggtcaggcc gatgccggcc gcgaccatca
      241 ccgaggagag caggagcagg gggctcaggc gatcgccggt caccgcggcg gcgatgagca
      301 cgccggcgag cggagtggtg agcgagcagg tggcgagcgc gctggccagg tacacctgga
      361 agaggtacga gttgaccacg aagttgaagc ccgcgatcac cgcgccctgg tagaagagcg
      421 aggtggcgag cccccaggtg taacgggtgg gggtcgcgcc ctcgaagacg aggctcgcga
      481 tcaggaagca ggcgctgccg atcgcggact ggaacaccag cagcttgatc ggatcgagcc
      541 gctgcaccgc gcgcgccatg tagacgaccc gctcccccag gagcaccgcg ctgcccatca
      601 ccaccacgtc accgagcagg gtcgagctct ggaacgagaa gtcgcgcgcg aagagcagca
      661 cgatgccggt gtaggcgacg agcaccccgc ccaccttggc cggggtgagc cgatcgcccg
      721 ggatcaggaa gtgggcgagc accacggtgt ggaccgcgta ggagttgagc agcaccgccc
      781 cgtgggccgc ggtggtgagc ccgagcccca cgttcatggt gccgatctgg acggagaaga
      841 tcaccccgag cgagatcagc acccgccact cgcccggccg caggtcgaag atgccgcggt
      901 gccgcgtcca ccacgcgtag gccaggatgg tgagcccgcc cagcacgaag cggaagaggg
      961 cgaggcgcag cggcggcgcg tccacgagtc cgatcttcac cgcgaccgga ttgccgcccc
     1021 agaggacggc gaggaagatc gcggtcgagg ccccgcgcca gtcgagcgga cggagcggca
     1081 ctaggaggcg cgctcgcggc gccgggggac ggccatatcg gcggctatgg tagcatgcgt
     1141 tccatgaaga tcgggctcat cgggcttccc aagagcggca agacgtcgct cttcaacctg
     1201 ctgaccggcg cgacggtggc cacctcgtcc ttcggcaccg cgcgcgggga gatgcacgcg
     1261 ggggtggcgc gcgtgccgga cgcgcgggtc gaccgcctcg tcacgctctt caagccgaag
     1321 aagatcacgc tcgccacctt cgaggtggtg gatctggccg gcatcgcgaa gggcgagcgc
     1381 cagggtctcg aggcgaagga gttccgcaac gccgacgcgc tgctgcacgt ggtgcgcgcg
     1441 ttcccggaca cgggcggcgc cgcgcccgac cccgcgggcg acatcgacga cctcgacacc
     1501 gagctgatcc tcgccgacct cgaggtggtg gagcgccggc tcgagcggct cgaggcccag
     1561 atcaagaaga agcgcaccga cgccgacgtg cacgagcagg cggtgctgat ccggatgaaa
     1621 cccgatctcg aggcggggcg tccgatccgc gcgatggggc tctccgacga cgagacgcgc
     1681 gcgatccgtg gcttcacctt cctctcgcag aagccgatcc tgcactgcct gaacctggcc
     1741 gagaaggaga tcgcgcgcgg caagaccgtc gtggagagct tcggcctcgc ggaggtcgcg
     1801 gcgcgcccgg gcacccgggc gggctgggtc tccgcggtca tcgagtccga ggtcgcgcag
     1861 ctcgaagggt cggagcaggc cgcgttcctc gccgatctgg gcctgaccga gccggccatc
     1921 cggcgcgtcc tcgaggattg ctacacgctg ctgggcctca tctcattctt cacggtgggt
     1981 gaggacgagg tgcgggcgtg gtcgatcccg cgcaacaccc gcgcgcagga cgcggcgagc
     2041 gccatccact ccgacatcgc gcgcggcttc atccgcgcgg aggtggtggg ctacgacgac
     2101 ctcatggccc tcgagggctc gatgtccgcc gcgcgcgacc gcggccgcct gcgcctggaa
     2161 ggcaaggact accaggtcca ggacggcgag atctgccact tccgcttcaa cgtcgccaag
     2221 tagggccgca gggcgaggcg cagccgcagg cgagccgagc gaaatccaga agggcagtaa
     2281 cgccccgccc tcatctccgc gcgtccgcgg tgccgcggcg gcagccgagc ggccaggtca
     2341 gcgcgagccg atcggtcggg tacgcgcgct cggcctcgaa gcagcccacc accagcggga
     2401 tcgggtcctg cgtgccgcgg aggtcgccgt cccgatgcga ggcgcaggcc accgtgcaca
     2461 gctcgaggtg cacgtggggg acgttggccg agttccccga ggtgccggag aacccgatga
     2521 cctgcccgcg cgcgacgacc tggccggtcg cgaccaccgc ccgcgtcagg tggcagtaga
     2581 ccgtccagcg gctgaaggcg gggtgggcga gcaccacccc gttcccgcag ccctgctcgt
     2641 agtcgatcag cagcgacacc gtcccgtcgg cggcggcgag aaccggtgtg cccaccggga
     2701 ccgcgatgtc gacgccggcg tgagggcgca ggcgcggcag ctcgttggca cccgcgagcg
     2761 agcggtaggc ggagaggatc gtcacgtccg gcgcgtcgtc ggcccgcgcg gcgccgacgg
     2821 gagcgagcag gagcatcgcg ccgagcatca ccgggaccgg gcccatgcgc tactggggcc
     2881 ggatcaagcc gagcttggga gccccgccga gggcccggat gacgctcacc agcatcgcgt
     2941 cggatacgcc gctcggcagg acgcagccgc cgcccacgat gagcccgcgc ccgccggtct
     3001 gctcgatcgc gtcccgcacc tgcgcctgcg cc
//
LOCUS       DATMCV010000477         2813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_625065, whole genome shotgun sequence.
ACCESSION   DATMCV010000477 DATMCV010000000
VERSION     DATMCV010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625065"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="VNU03_11025"
     CDS             <1..1555
                     /locus_tag="VNU03_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWP75042.1"
                     /translation="QVDEFAKITGVAYVMVYDPQRGFIAHTFAPVVPKGIVEKNVVPG
                     HAARPQVREIGYPDPRTGREREIIDIGVPMLGGQLGTVRVGMDKAIITAASVQAGNFL
                     LLVFGGIAIAAGLAGVIFAQRITRPVTALMAGAERVGRGDLSQLVPVTSRDEIGQMAV
                     TFNESIVRLRALVQTEADRDEERRKREDLQQNVIKFLDTVVEIGKGDLTRRGEVTWDV
                     LGNVVDAINLMVEEIATVVGDVRQAAIRVTAGSQDTIKITGELAMAAQTQSRDASSVT
                     RSVSDVMVSVRQVADSADQSAKAARQVLQAASKGEEAVRNSLSSMQQIRAEVQTISKK
                     IKSLGDRSLEISDIVNTIQDIAAQTHLLALNASIEAAGAGEAGLRFSVVADEVRKLAE
                     RATQATRDIGTLIKGVQMETQEAVVAMESGTHEVESGYEVSLKAEEALQEIGKISQTS
                     SELAAEISQASQRQVRGTEAMAGAVQAIAGAAVKTEQGVQQSRSNVEQLARLAEELTV
                     KLSRFKLES"
     gene            1564..>2813
                     /locus_tag="VNU03_11030"
     CDS             1564..>2813
                     /locus_tag="VNU03_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWP75043.1"
                     /translation="MAERRERELMLGIFLMEAWDTAGALEEGLDQLTGSEAPTVATLA
                     PLVVLAHRLKGSAALYGFPGVSDLAGAAERLLERAAAASAEDRARAAGFLGGLVGLLK
                     EVFDGISAVGVEDVARVGEFKARYPTFLAAAPADGRGIGAMPSAAAAGPAPVRSPVAA
                     ALDRFFADGNEVLEYFLPEASEHLEAMTTALLAVEGGARDDETLATVFRSVHTLKGAA
                     YTVGCAPIGDAAHQIEDLLGAIRENRLAMTPAVTEAIFVGTTAIKQVLQTGITVSEDT
                     ALALEGATQDLRALMASVVGDRSAPVMAQEDLLTQVAAPEMETAPASPVAPLVPVLPG
                     FVRPEFLARLEALPRPALRSLSAPTGPSIRVAVERLDTLMNLVGELMIARSRLDRRLG
                     QLDQVSQLLLASRTRLGQVGRDFE"
BASE COUNT          436 a          919 c         1035 g          423 t
ORIGIN      
        1 ccaggtggac gagttcgcga agatcaccgg tgtcgcctac gtgatggtct acgatccgca
       61 gcgcgggttc atcgcccaca ccttcgctcc ggtggtgccc aagggtatcg tcgagaagaa
      121 tgtggtcccc gggcacgccg cgcgtcctca ggtgcgggag atcggctatc cggatccccg
      181 gaccggccga gagcgcgaga tcatcgatat cggggtgccc atgctgggcg ggcagctggg
      241 gacggtgcgg gtgggcatgg acaaggcgat catcaccgcc gcgtcggtgc aggcgggcaa
      301 cttccttctg ctggtgttcg gcgggatcgc gatcgcggcg ggcctggccg gggtgatctt
      361 cgcgcagcgt atcacccgac cggtgaccgc tctgatggcc ggggccgagc gggtggggcg
      421 gggggacctc tcgcagctgg tgccggtcac gtcccgcgat gagatcgggc agatggcggt
      481 cacgttcaac gaatccatcg tgcgtctccg ggcgctcgtg cagaccgagg ccgatcgcga
      541 cgaggagcgg cggaagcgcg aggacctgca gcagaacgtc atcaagttcc tcgatacggt
      601 cgtcgagatc ggaaagggag acctcacccg acgcggcgag gtgacctggg acgtgctcgg
      661 caacgtggtc gacgccatca acctgatggt cgaggagatc gccaccgtcg tcggcgacgt
      721 ccgacaggcc gcgatccggg tcaccgccgg ctcccaggac accatcaaga tcaccggcga
      781 gctcgcgatg gccgcgcaga cccagtcccg cgacgcgtcg agcgtcacgc gatccgtatc
      841 ggacgtgatg gtgtcggtgc gccaggtcgc tgacagcgcg gatcagagcg ccaaggccgc
      901 gcgccaggtg ttgcaggccg ccagcaaggg cgaggaagcg gtgcgcaaca gcctgtcgag
      961 catgcagcag atccgggccg aggtgcagac gatctcgaag aagatcaagt cgctcggcga
     1021 tcgctccctc gagatctccg acatcgtgaa caccatccag gacatcgcgg cccagaccca
     1081 tctcctggcc ctcaacgcct cgatcgaggc ggcgggagcc ggcgaggcgg gtctgcgctt
     1141 ctcggtggtc gccgacgagg tccggaagct cgccgagcgg gccacccagg ccacccgcga
     1201 catcggcacc ctgatcaagg gcgtgcagat ggaaacgcag gaagcggtgg tcgcgatgga
     1261 gtccggcacc cacgaggtcg agtcgggcta cgaggtgagc ctcaaggcgg aggaggccct
     1321 ccaggagatt ggtaagatct cgcagacctc ttccgagttg gcggccgaga tctcccaggc
     1381 ctcccagcgg caggtccgcg ggacggaggc gatggcgggg gcggtccagg ccatcgccgg
     1441 agcggcggtg aagaccgagc agggcgtcca acagagccgg agcaacgtcg agcagctggc
     1501 ccggctggcc gaggagttga ccgtcaagct ctcccgcttc aagctagaga gttgaggacc
     1561 cgcatggcgg aacggcgcga gcgcgagctg atgctgggga tcttcctcat ggaggcctgg
     1621 gataccgccg gggctctcga ggagggtctg gatcagctga ccggctccga ggctccgacg
     1681 gtcgcgaccc tggctcctct cgtggtgctc gcgcatcgtc tgaaggggtc cgcggcgctc
     1741 tacggcttcc cgggcgtgtc ggatctcgcc ggcgccgcgg agcgactgct cgagcgggcg
     1801 gccgccgctt ccgccgagga ccgggctcgc gcggccgggt tcctgggcgg cctggtgggc
     1861 cttctgaagg aagtcttcga cgggatcagc gcggtcgggg tggaggacgt ggccagggtg
     1921 ggcgagttca aggcccgcta tcctacgttt ctcgcggccg ctcccgccga cgggcggggg
     1981 atcggcgcga tgccttcggc tgcggcggcg gggcccgccc cggtccggtc gccggtcgcc
     2041 gccgcgctcg accgcttctt cgccgacggc aacgaggtgt tggagtactt cctgcccgag
     2101 gcctccgagc acctcgaggc gatgaccacc gccttgctgg ctgtcgaggg cggcgcgcgg
     2161 gacgacgaga cgctggcgac cgtcttccgg tcggtgcaca cgctgaaggg cgccgcctac
     2221 acggtcgggt gcgcgccgat cggcgacgcc gcgcatcaga tcgaggatct gctcggcgcc
     2281 attcgtgaga accggctcgc gatgactccg gcggtcacgg aagcgatctt cgtggggacg
     2341 accgcgatca agcaggtgct ccagacgggc atcacagtct ccgaagatac ggcgctcgcc
     2401 ctggaggggg ccacccagga cctccgcgct cttatggcgt cggtggtcgg cgaccgttcg
     2461 gcgccggtaa tggcccagga ggatctcctc acgcaggtgg ccgctcccga gatggagacg
     2521 gcgcccgctt ccccggttgc tccccttgta ccggtgctgc cggggttcgt acgaccggag
     2581 ttcctggccc ggctggaggc gttgccccgg ccggccctgc gctcgctcag tgccccgacc
     2641 gggcccagca tccgcgtggc ggtcgagcgc ctcgacaccc tgatgaatct ggtcggcgag
     2701 ctgatgatcg cgcgaagccg cctcgaccgg agactgggcc agctcgatca ggtgagccag
     2761 ctgctcctcg ccagccgaac gcggctcggg caagtggggc gcgacttcga gtt
//
LOCUS       DATMCV010000478         3351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_892877, whole genome shotgun sequence.
ACCESSION   DATMCV010000478 DATMCV010000000
VERSION     DATMCV010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892877"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VNU03_11035"
     CDS             <1..194
                     /locus_tag="VNU03_11035"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl sulfatase C-terminal domain-containing
                     protein"
                     /protein_id="HWP75044.1"
                     /translation="EQPDATVTLRRPVLNRLVLRELTIAGAIDQGLVAVAGDRAPVAL
                     LFALLDDFALMFDVIEPRR"
     gene            complement(208..1023)
                     /locus_tag="VNU03_11040"
     CDS             complement(208..1023)
                     /locus_tag="VNU03_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWP75045.1"
                     /translation="MRSGRLRDGLVLAALALYALPFFWQALTSVRPDAELLPLSHLLP
                     SRLTAVHYEVVLRQSLMPRALLNSLGVALSSTLLAVTLGALAGYALARLPVPGRGVIL
                     LGMIAATAFPQIATVSPLYLLMRAFGLRDTWLALILANASFALPLVIWLLAGFIREIP
                     RALEEAASVDGAGRLASLRFVVLPLVAPGMASAALLAFLFSWNEFLFAYSFTATEASR
                     TVPVALALFPGVFEVPWGDIAAASLLASLPPILIVVAFQRYLVRGLLAGALRE"
     gene            complement(1020..1877)
                     /locus_tag="VNU03_11045"
     CDS             complement(1020..1877)
                     /locus_tag="VNU03_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWP75046.1"
                     /translation="MTDRRLGWLLLAPALALLGGLTVYPAVWVIWLSLQYRIPVFSIS
                     RWAGLAHYAFLAVDTRFWNAASVTAVFTVASVALEGGLGLAMALGLRAQLHGRRAAVA
                     LLLLAWALPSVVTAKMFEWLYHPAGGLVNFLLGGRPVNWLGDPALALPSLIVADVWRT
                     TPFVALLCYARLLTIPGELYEAAEVDGAAGVQAFARITWPLVRPILLVALLFRTLDAL
                     RAFDLMFVLTGGGPAGTTETLTVYAYRALFQTLQVGFGSAIGVVVFALVMVVAVAYLR
                     MIHRQEASA"
     gene            complement(1874..3166)
                     /locus_tag="VNU03_11050"
     CDS             complement(1874..3166)
                     /locus_tag="VNU03_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75047.1"
                     /translation="MAPRPARAAAALVVLLVLLTAAACRERPPSGGPVTLVFKHAKIL
                     GPADPVPGLLRRFEADHPGVQVQSEALTWSSDEQHQFYVINLEGGSPPFDVMMLDVIW
                     VPEFARAGWILDLTARVPAAEREAHFASAIEPAVQGDRLWALPWFMNVGLLYYRKDLL
                     AKYGLAPPETYDELVRQVRLVQAGERDRRLDGYLWQGKQYEGGMVNVLEALWANGTRL
                     LDETGRPFPEPARAREALAFLRSLIESGVSPAWVTAADEELTRRPFGDGRAIFLRGWP
                     YALDLFELPDSRVRGRVGVAPMPRLAHGTVGAASTGGAHLAVSARTRHAALAVELVRF
                     LTGEPAQRAMTEGAALRPSRPALYRDAGLIARDPTLPALLPLILKGHPRPVTPYYLML
                     STTLQPEFSAALVGRKSPERAIRDGKAQVEHLQQALSR"
BASE COUNT          539 a         1194 c         1267 g          351 t
ORIGIN      
        1 gcgagcagcc cgacgcgacg gtgacgctcc ggcgcccagt gctgaaccgg ctcgtgctgc
       61 gcgagctgac catcgcgggg gcgatcgacc agggcctggt ggccgtcgcg ggcgatcggg
      121 ccccggtcgc cctgctcttc gcgctcctcg acgacttcgc gctgatgttc gacgtgatcg
      181 agccgcggcg ctgagggcgg agtggcccta ctcgcgcagc gcgcccgcga gcagcccgcg
      241 cacgagatac cgctggaagg cgaccacgat caggatgggc ggcaggctcg ccagcagcga
      301 ggccgccgcg atgtcgcccc acggcacctc gaagacgccg ggaaagagcg ccagcgccac
      361 cggcaccgtg cggctcgcct cggtggcggt gaagctgtag gcgaagagga actcgttcca
      421 ggagaagagg aaggccagca gggcggccga ggccatcccg ggcgccacca gcgggagcac
      481 cacgaagcgc aggctcgcga ggcgccccgc cccgtcgacg gacgccgcct cctcgagggc
      541 gcgcgggatc tcgcggatga acccggcgag cagccagatc accagcggca gcgcgaacga
      601 cgcgttggcg aggatcagcg cgagccaggt gtcgcggagg ccgaaggccc gcatcaggag
      661 atagagcggg ctcacggtgg cgatctgggg gaacgcggtc gccgcgatca tgcccaggag
      721 gatcaccccg cggccgggga ccggcaggcg cgcgagcgcg tagcccgcga gggcgccgag
      781 cgtcaccgcg agcagggtgc tcgagagcgc gacccccagg ctgttcagca gcgcgcgcgg
      841 catgaggctc tgccgcagca ccacctcgta gtgcaccgcg gtgagccggc tcggtaggag
      901 atgactgagc ggcaggagct ccgcgtccgg ccgcaccgag gtgagcgcct gccagaagaa
      961 cggcagcgcg tagagcgcca gcgcggccag caccaggccg tcgcggagcc ggccgctcct
     1021 cacgcggacg cttcctgccg gtggatcatc cgcagatagg cgaccgccac caccatcacc
     1081 agcgcgaaca cgaccactcc gatggcggag ccgaagccca cctgcagggt ctggaagagc
     1141 gcgcggtagg cgtacacggt gagggtctcg gtggtgccgg cggggccgcc gccggtcagc
     1201 acgaacatga ggtcgaacgc ccgcagcgcg tcgagcgtcc ggaagaggag cgccaccagg
     1261 aggatcggcc gcaccagcgg ccaggtgatg cgggcgaacg cctgcacgcc cgcggcgccg
     1321 tccacctccg ccgcctcgta cagctcgccc gggatggtca agagccgcgc gtagcagagc
     1381 agggccacga acggggtggt gcgccagacg tcggccacga tcagcgaggg cagcgccagc
     1441 gccgggtcgc cgagccagtt gaccgggcgg ccgcccagga ggaagttcac gaggccgccc
     1501 gccggatgat agagccactc gaacatcttg gcggtcacca ccgacggcag cgcccacgcc
     1561 agcaggagca gggccaccgc cgcgcgccgc ccgtggagct gggcccgcag gccgagggcc
     1621 atcgcgaggc cgagcccgcc ctcgagcgcg accgacgcca ccgtgaacac cgcggtgacc
     1681 gaggccgcgt tccagaatcg cgtgtccacc gccaggaagg cgtagtgggc gaggccggcc
     1741 cagcgcgaga tcgagaacac cgggatccgg tactggagcg agagccagat cacccagacc
     1801 gcgggataga cggtgaggcc gcccagcagg gccagcgcgg gggcgagcag gagccacccg
     1861 agccgccggt cggtcatcgc gagagcgcct gctgcaggtg ctcgacctgg gccttgccgt
     1921 cccggatcgc gcgctcgggc gacttgcgcc ccacgagcgc ggccgagaac tcgggctgca
     1981 gcgtggtcga cagcatgagg tagtagggcg tcaccgggcg tgggtgcccc ttcaggatga
     2041 ggggcaggag cgcgggcagc gtcggatcgc gtgcgatcag gcccgcgtcg cggtagagcg
     2101 cggggcgcga ggggcggagc gccgcgccct cggtcatcgc gcgctgggcc ggctcgcccg
     2161 tgaggaagcg caccagctcg accgcgagcg cggcgtggcg ggtgcgcgcg ctcaccgcca
     2221 ggtgcgcgcc gccggtggac gcggcgccga ccgtcccgtg cgcgaggcgc ggcatcggcg
     2281 ccaccccgac ccggccgcgc acgcgcgagt ccggcagctc gaagagatcg agcgcgtagg
     2341 gccagccgcg caggaagatg gcccgcccgt ccccgaacgg gcggcgggtc aactcctcgt
     2401 cggcggcggt gacccacgcc gggctcaccc cgctctcgat gagcgagcgg aggaacgcga
     2461 gcgcttcccg ggcccgcgcc ggctccggga acggccggcc ggtctcgtcg agcaggcggg
     2521 tgccgttggc ccagagcgcc tccagcacgt tgaccatgcc gccctcgtac tgcttgccct
     2581 gccacagata gccgtccagg cggcgatcgc gctccccggc ctggacgagc cgcacctgcc
     2641 gcaccagctc gtcgtacgtc tccggcggcg cgagcccgta cttggcgagc agatccttcc
     2701 ggtagtagag caggcccacg ttcatgaacc acggcagcgc ccagagccgg tcgccctgca
     2761 ccgcgggctc gatggcggag gcgaagtgcg cttcgcgctc ggccgcgggc acgcgcgcgg
     2821 tcaggtcgag gatccagccc gcccgcgcaa actcgggcac ccagatcacg tcgagcatca
     2881 tcacgtcgaa gggcgggctg ccgccctcga ggttgatcac gtagaactgg tgctgctcgt
     2941 cggaggacca ggtgagcgcc tcgctctgca cctgaacccc ggggtgatcg gcctcgaaac
     3001 ggcgaaggag gccgggcacc ggatcggcgg ggccgaggat cttggcgtgc ttgaagacga
     3061 gcgtgaccgg cccgccgctc ggcgggcgct cgcggcacgc ggccgcggtc agcaggacga
     3121 gcaggacgac gagagcggcc gcggcgcgcg ccggacgggg cgccatgggc gagggtcgcg
     3181 gccgcccggc tatccggcgc ccagcaggtc acgcagggcg ccgccctcgg ccttgaacca
     3241 gtcgaagagg atcgacacgt cccaacccat gcagaagtgc cgcaccccca gatcgaggta
     3301 ggccttcgcc tcgttcgggt gcgagatctc ctcgcgcggg gcgatgccga g
//
LOCUS       DATMCV010000479         5819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_625083, whole genome shotgun sequence.
ACCESSION   DATMCV010000479 DATMCV010000000
VERSION     DATMCV010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625083"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VNU03_11055"
     CDS             complement(<1..247)
                     /locus_tag="VNU03_11055"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWP75048.1"
                     /translation="MPKHLDMIRQIQRGEAPPPPVASLIGFTLTEVEPGRAVVALEAD
                     RRHANPMGTVHGGILCDIADAAMGIAYAATLDEGETFT"
     gene            322..780
                     /locus_tag="VNU03_11060"
     CDS             322..780
                     /locus_tag="VNU03_11060"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP75049.1"
                     /translation="MPKRYNQPCPVAKSLEVIGDRWTLLVVRDLLRGTQRFQDLLTTL
                     PGIAPNILSARLKLMEEHGLIVREFYSDHPPRAQYALSAKGRELGMVVGALAAWGGRH
                     VHKQTKLVHSGCGHPVSVGYYCPDCGEGVRGADVTVTRARPRPGTRARRG"
     gene            complement(734..1549)
                     /locus_tag="VNU03_11065"
     CDS             complement(734..1549)
                     /locus_tag="VNU03_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP75050.1"
                     /translation="MAHFTTSDGVRIYYESHGTGDPLFLAYGIGGNAGMWQPNVHALA
                     DRHRLVLWEPRGHARSESPADPTRVTFGHWVLDLRDLLEHLGVTHAVVGGLSLGGGIA
                     TRFALAHPERVRALVVVDSSSAVGLPLSVENVVMRARSIEVTLKGGMDAMAEFAIASN
                     PNVAGRLKLDPGARQEIFDYYRMLTPIGYANALRALLQMDYITERLPEISAPTLLVCG
                     DEDPSLAPMRVMEKKIKQARFVLLSPAGHFANRDQPEAFNRAVLEFLDEVGPA"
     gene            1588..3687
                     /locus_tag="VNU03_11070"
     CDS             1588..3687
                     /locus_tag="VNU03_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HWP75051.1"
                     /translation="MERSFAEEVKQLALGEGQVLRGEGILAITKALLQAGVSYVGGYP
                     GAPVSHLLDVLADANESLLKPMGVYLDASANEAAAAALLGASINYPMRGAVTWKSIVG
                     TNVAADALSNLASAGVIGGALIVVGEDYGAGASVIQERTHAFALKSSLILIDPQPTLQ
                     NLARMVEEGFGISEASNLPAVISLRIRAAHMRGSLVCRDNVRPAVGMNSPLERHSFDY
                     SRLSHPPSTYTQEAQKFERRLPAAQRYIVERGLNEVRPGEEKGLGIILQGGLTPTALR
                     ALELLGAADAYGQSRIPMLVLNVIHPLVPDQLVAFLEGKQRVLVLEEGMPSFIEQELK
                     ALAYDHGITTRIHGKDVVAAQGEYVPEVVLGALARFLGGDAERRRQALTAHVGQGRAQ
                     LRTPLPKRPPAFCTGCPERPVFSALKIAQRELGETHVAADIGCHAFATLPPFNQGNTI
                     LGYGMSVASASAVAPNFDKRVISILGDGGFWHSGLTAGVANAVFNKQDSIVVVLENFY
                     TSATGQQANPSSGKNPRGDAVSMSISETIRSLGVSWIKTVNPYRVADTLKVIKDAMTT
                     ATGGLKVIIARAECQLERQRRVKPETAARLKAGGRVEVPRFGVDPDVCTGDKSCMRFN
                     GCPSLTLKESQDPLRDDPVAHVDATCVGCGLCGEVAHAAVLCPSFYEARVVHNPGAWA
                     RGLATARRRVIGWLQPA"
     gene            3687..5195
                     /locus_tag="VNU03_11075"
     CDS             3687..5195
                     /locus_tag="VNU03_11075"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HWP75052.1"
                     /translation="MASRRPVSILIAALGGQGGGVLTEWIVGAAAHAGYPAQATSIPG
                     VAQRTGATTYYVEVFPERAEPGSPEPVFSLYPTPGDVDVIIASEMLEAGRTIETDYAS
                     PGRTTVIASTHRLFSIAEKSAPGDGVFPREALEAAARALSRRFVGFDGLALARRHQTE
                     VNALLLGALAASEIVPMTAADFEAAIREGGVAVDRNVRGLKAGVELVAAGPGDGEAPR
                     SDRPWADVKAERARGLGARGAAFLALATRAEAELPEPLHAVLGEALARLVDYQDARYA
                     ALFLERVRRVRALAPGDRLTRVFARRLAVWMTYEDAIRVADLKTRRGRFERIRRENAA
                     PEGSVLVVTDYLKPDLDELYGILPAAIGGPIARWAERRWPDGRPTIGQHVRTTTVLGF
                     LRVWLLARLRALRPASLRRQREFALIARWEETVLAAAPLDESLACEVAELGNVVKGYG
                     EVRRRLSGALARFLDDTARPAIEADRRAGTGFARSTDTIRAGRLHLLSDEKG"
     gene            5199..>5819
                     /locus_tag="VNU03_11080"
     CDS             5199..>5819
                     /locus_tag="VNU03_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWP75053.1"
                     /translation="MTNVLLTRSGPIATVTLNRPDRRNSLSDDMLTELGAAFAELRDD
                     AATRVVIVTGAPPVFSAGADAGHAKASTPEERRRLFLSRKSQFRRLFERANAVLENLE
                     QATIAAVNGHAVGGGWGLALACDFRIAAAEAQFWIPEVDLGVPLGVGTTTRFVRMVGP
                     ARAKEIIMGCARYSAADAQTWGLVHRVVPGAGLAAAVADYAAGLAKK"
BASE COUNT          799 a         2098 c         2110 g          812 t
ORIGIN      
        1 tggtgaaggt ctcgccctcg tcgagggtcg ccgcgtaggc gatgcccatc gcggcgtccg
       61 cgatgtcgca cagaatcccg ccgtggaccg tgcccatcgg gttggcgtga cgccggtccg
      121 cctcgagcgc gacgaccgca cgtccgggtt cgacctcggt gagggtgaac ccgatcaggc
      181 tggcgaccgg cgggggcggc gcctcgccgc gctggatctg ccggatcatg tcgaggtgct
      241 tgggcatcag tgtcgtcctc cagtgactat taaaataata gtgacttgct cgcttgtcaa
      301 gcgggccggt atactgagcg catgccgaag cgctacaacc agccctgccc ggtggcgaag
      361 tccctggagg tcatcgggga tcgctggacc ctgctggtgg tgcgcgacct gctgcggggg
      421 acccagcgct tccaggacct cctgaccacg ctgcccggca tcgcccccaa catcctctcc
      481 gcccgcctca agctgatgga ggagcacggg ctcatcgtgc gggagtttta ctcggaccac
      541 ccgccgcgcg cccagtacgc gctcagcgcc aagggacgcg agctcggcat ggtggtgggt
      601 gcgctcgccg cgtggggcgg tcgccacgtg cacaagcaga ccaagctcgt gcactccggg
      661 tgcggccacc cggtctcggt cggctactac tgtccggact gcggggaagg ggtgcggggc
      721 gcggacgtca ccgtcacgcg ggcccgacct cgtccaggaa ctcgagcacg gcgcggttga
      781 aggcctccgg ctggtcccgg ttggcgaagt ggccggccgg ggagagcagc acgaagcgcg
      841 cctgcttgat cttcttctcc atcacccgca tcggggcgag cgacgggtcc tcgtcgccgc
      901 agaccaggag ggtgggcgcc gagatctcgg gaagccgctc cgtgatgtag tccatctgga
      961 gcagggcgcg cagcgcgttg gcgtacccga tgggggtcag catccggtag tagtcgaaga
     1021 tctcctgccg ggcccccggg tcgagcttga gccgccccgc cacgttcggg ttcgacgcga
     1081 tggcgaactc ggccatggcg tccatgccgc ccttgagcgt gacctcgatc gagcgggccc
     1141 gcatcaccac gttctcgacc gacagcggca ggcccaccgc ggaggacgag tccaccacca
     1201 ccagggcccg cacccgctcc gggtgggcca gggcgaaccg ggtcgcgatg ccgcccccca
     1261 gcgagaggcc ccccaccacc gcgtgggtca ccccgaggtg ctcgagcagg tcgcggaggt
     1321 cgagcaccca gtggccgaag gtgacccggg tggggtcggc cgggctctcc gaccgggcgt
     1381 ggccgcgggg ctcccagagc accaggcgat gccggtcggc cagggcgtgg acgttgggct
     1441 gccacatccc cgcgttgccg ccgatgccgt aggccagaaa cagcggatcg ccggttccgt
     1501 gggactcgta gtagatgcgg acaccgtcgc tcgtcgtgaa gtgcgccacg ggctcctccc
     1561 ccgagggttg tggtacaaga gggttctatg gaacggtcct tcgccgagga agtcaagcag
     1621 ctcgcgctgg gagaggggca ggtgctgcgc ggcgagggca tcctggccat caccaaggcc
     1681 ctgctgcagg cgggcgtctc ctacgtgggc ggctacccgg gcgcgccggt ctcgcacctg
     1741 ctcgacgtcc tggccgacgc caacgaatcg ctgctgaagc cgatgggcgt ctatctcgac
     1801 gcctcggcca acgaggcggc cgccgccgcc ctgctcggcg cgtccatcaa ctacccgatg
     1861 cgcggcgcgg tgacctggaa gtccatcgtg ggcaccaacg tggccgcgga cgcgctctcc
     1921 aacctggcct cggccggggt gatcggaggc gcgctcatcg tggtcgggga ggactacggg
     1981 gcgggggcca gcgtgatcca ggagcgcacg cacgccttcg cgctcaagtc gtcgctgatc
     2041 ctgatcgatc cgcagccgac cctgcagaac ctggcccgca tggtcgagga gggcttcggc
     2101 atctccgagg cctcgaatct cccagcggtc atcagcctcc ggattcgcgc ggcgcacatg
     2161 cgcgggagcc tggtctgccg ggacaacgtg cgcccggccg tcggcatgaa ctccccgctc
     2221 gagcgccact cgttcgacta ctcgcggctg agtcacccgc cgtcgaccta cacccaggag
     2281 gcccagaagt tcgagcgccg cctccccgcc gcgcagcgct acatcgtcga gcgcgggctc
     2341 aacgaggtgc ggcccggcga ggagaagggg ctcggcatca tcctgcaggg cgggctcacc
     2401 ccgaccgcgc tgcgcgcgct cgagctgctg ggcgcggccg acgcctacgg ccagagccgc
     2461 atcccgatgc tggtgctgaa cgtgatccac ccgctggtgc cggatcagct cgtggccttc
     2521 ctcgagggca agcagcgggt gctggtgctc gaagagggca tgccgagctt catcgagcag
     2581 gagctgaagg cgctggccta cgatcacggg atcaccaccc gcatccacgg caaggacgtg
     2641 gtggcggcgc agggcgagta cgtgcccgag gtggtgctgg gcgccctcgc gcgcttcctg
     2701 ggcggcgacg cggagcggcg gcggcaggcc ctcaccgccc acgtggggca ggggcgggcg
     2761 cagctgcgga cgcccctgcc caagcggccg cccgccttct gcaccggctg ccccgagcgc
     2821 ccggtgttca gcgcgctcaa gatcgcgcag cgggagctgg gcgagaccca cgtggccgcc
     2881 gacatcggct gccacgcctt cgcgactctc ccaccgttca accagggcaa caccatcctg
     2941 ggctacggca tgagcgtcgc ctccgcgagc gcggtggcgc cgaacttcga caagcgggtc
     3001 atctcgatcc tgggcgacgg cggcttctgg cactccggcc tcaccgcggg ggtggccaac
     3061 gcggtcttca acaagcagga ctcgatcgtg gtggtgctgg agaacttcta cacctcggcc
     3121 accgggcagc aggccaatcc gtcgtctggc aagaacccgc gaggcgacgc ggtgtccatg
     3181 tcgatctcgg agaccatccg gagcctcggg gtgtcctgga tcaagacggt gaacccgtac
     3241 cgggtcgccg ataccttgaa ggtgatcaag gacgcgatga ccaccgcgac cggcgggctc
     3301 aaggtgatca tcgcgcgcgc ggagtgccag ctcgagcgcc agcggcgggt caagccggag
     3361 accgcggcgc ggctcaaggc gggcgggcgc gtcgaggtgc cgcgcttcgg ggtcgatccc
     3421 gacgtgtgca ccggcgacaa gtcgtgcatg cgattcaacg gctgcccgtc gctcaccctc
     3481 aaggagagcc aggatcccct gcgcgacgac ccggtggccc acgtggacgc gacctgcgtc
     3541 ggctgcgggc tctgcggcga ggtggcgcac gccgcggtcc tctgcccctc gttctacgag
     3601 gcgcgcgtcg tccacaaccc gggcgcctgg gcccgcggcc tcgcgaccgc gcggcgccgg
     3661 gtgatcggct ggctgcagcc ggcgtgatgg cgtctcggcg cccggtctcg atcctgatcg
     3721 ccgccctcgg cgggcagggc gggggcgtgc tcaccgagtg gatcgtggga gcggcggccc
     3781 acgcgggcta cccggcccag gccacctcga tcccgggcgt ggcccagcgc accggcgcca
     3841 ccacctacta cgtcgaggtc ttccccgagc gcgcggagcc gggaagcccc gagccggtgt
     3901 tctcgctcta tcccacaccg ggtgacgtgg acgtgatcat cgcctccgag atgctcgagg
     3961 cgggacgcac gatcgagacg gactatgcgt ctcccggccg gaccaccgtg atcgcgagca
     4021 cgcaccggct cttctcgatc gccgagaagt cggcgccggg cgacggggtg ttcccgcgcg
     4081 aggcgctcga ggccgcggcc cgcgcgctga gccgccgctt cgtgggcttc gacgggctgg
     4141 ccctcgcgcg acggcaccag accgaggtca acgcgctcct cctgggcgcc ctcgcggcca
     4201 gcgagatcgt gccgatgacc gcggccgatt tcgaggccgc gatccgcgag ggcggcgtcg
     4261 cggtggaccg gaacgtgcgc gggctcaagg ccggcgtgga gctggtcgcg gccggtcccg
     4321 gcgacggaga ggcgccgcgc tccgatcgcc cctgggcgga cgtgaaggcc gagcgcgcgc
     4381 gcggcctcgg cgcgcgaggc gccgcgttcc tggccctggc gacccgggcc gaggccgagc
     4441 tccccgagcc gctgcacgcg gtgctgggcg aggcgctcgc gcggctggtg gactaccagg
     4501 acgcgcgcta cgcggcgctg ttcctcgagc gggtgcggcg ggtgcgcgcg ctcgccccgg
     4561 gagaccggct cacgcgggtc ttcgcgcgcc ggctcgcggt gtggatgacc tacgaggacg
     4621 cgatccgcgt ggccgacctc aagacccgcc gcgggcggtt cgagcggatc cgccgggaga
     4681 acgcggcgcc cgagggctcg gtcctggtgg tcacggacta cctcaagccc gatctcgacg
     4741 agctgtacgg catcctgccc gccgccatcg gcggcccgat cgcccgctgg gccgagcggc
     4801 gctggcccga cggccgtccc accatcggcc agcacgtccg caccaccacg gtgctcggct
     4861 tcctgcgcgt ctggctgctg gcccgactcc gtgcgctgcg gccggcgtcg ctgcggcggc
     4921 agcgggagtt cgccctcatc gcccggtggg aggagacggt gctcgcggcc gcccccctcg
     4981 acgagagctt ggcgtgcgag gtggcggagc tgggcaacgt ggtcaagggc tacggcgagg
     5041 tccggcgccg tctctccggc gcgctcgcgc gcttcctcga cgacaccgcg cgccccgcga
     5101 tcgaggccga ccgccgcgcg ggcacgggct tcgcccgctc caccgacacg atccgcgccg
     5161 gccgcctcca cctcctctcc gacgaaaagg gctgagtcgt gaccaacgtc ctcctgaccc
     5221 gctcgggccc catcgccacc gtgacgctga accgccccga ccgccgcaac tcgctgagcg
     5281 acgacatgct caccgagctg ggcgccgcct tcgcggagct gcgcgacgac gcggcgaccc
     5341 gggtggtgat cgtgaccggc gccccgcccg tcttctcggc cggcgccgac gcgggccacg
     5401 ccaaggcgtc cacgccggag gagcggcggc gcctcttcct ctcgcgcaag agccagttcc
     5461 ggcgcctctt cgagcgtgcc aacgcggtcc tcgagaacct cgagcaggcc accatcgccg
     5521 cggtgaacgg ccacgcggtg ggaggggggt ggggcctggc cctggcttgt gatttccgga
     5581 tcgcggcggc cgaagcccag ttctggatcc cggaggtgga cctgggcgtc ccgctcgggg
     5641 tgggcaccac gacccgcttc gtccggatgg tcgggcccgc gcgcgccaag gagatcatca
     5701 tgggctgcgc gcgctattcc gccgccgacg cccagacgtg gggcctcgtc caccgggtgg
     5761 tgccgggcgc ggggctcgcc gccgcggtcg ccgactatgc ggcggggctc gccaagaag
//
LOCUS       DATMCV010000480         3204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_360301, whole genome shotgun sequence.
ACCESSION   DATMCV010000480 DATMCV010000000
VERSION     DATMCV010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360301"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VNU03_11085"
     CDS             complement(<1..1167)
                     /locus_tag="VNU03_11085"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWP75054.1"
                     /translation="MNTTSMLAEFVTKSRLEDCPEAALDGARRAILDCLGVMLAGSIE
                     PAARILQQVAQAEGGLPLATVVGTGRRTGAVWAALCNGTAAHALDFDDTNFALMGHPS
                     APVLAASLAAGELALADGRAVLHAFLLGFEVETTLAEVINPAHYEHGWHATCTLGTLG
                     AAAAAARLLGLDAVQTRHALAVAASQSSGLKENFGTMTKPFHAGHAARSGVLSALMAR
                     EGWTASEHAIEGPQGFFAVLGAGKRALEPLVTLGAPWKILTTGVAVKPYPSCACTHSI
                     IDGALELRRVQGIRADDVEELTVGVCAGVPRILIHSRPRTGLEAKFSAEFSAAAALAD
                     GRVGMATFQDDRAQDPAIRRLMERVRVVVDPDIPGDLERHMWTRLTVRLKDGRQA"
     gene            1269..2594
                     /locus_tag="VNU03_11090"
     CDS             1269..2594
                     /locus_tag="VNU03_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75055.1"
                     /translation="MRRLAWCLTVVAIILAAAPARAQLFVASRPDPPFTIGPLMVRAT
                     VTEGPGPVPVVVGFSLQLAPNRTPADVAQDVFLLWPGEVVNAAPDRKADATLARYVTD
                     QGFEITGEGHVKLMARNLGDAGTVEALPAGAPFVTFVQTGPLGLSGPATFVRIPWSPR
                     LADRSWLMELTLDAAGLIKPVKVGWAERLVRGTHYRVAVAFHEVRDRPLFPMYFAHRD
                     RVVRLADAPAELVVQFPQSERLKIDDVYPPTAIRRLSETLETTEVVSLFLDRSDGITP
                     QQLAVQFGYFSRTQTALLVAAPLLIFALGQAMGPLLGRGLLRVIDVVSARVQLGGWRV
                     GGRDREQGVILPRETLERIAPGKTTREEVLRLCGTEMERQDQLSGRTTLIYRGRRLVP
                     EAHRIFGWLSTVRRWDVERHEVRIELDGDLVRDVQAEVRRYRLGAEEAR"
     gene            2710..>3204
                     /locus_tag="VNU03_11095"
     CDS             2710..>3204
                     /locus_tag="VNU03_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HWP75056.1"
                     /translation="MKCPRCQSQNREGVRFCEECGGRLVTSCASCGAELLPDKKFCGS
                     CGSPATPEPVERQPSTQAYTPKHLAEKILTSRHALEGERKQVTVLFADLKGSMELLAD
                     RDPEEARRLLDPVLERMMEAVHRYEGTVNQVMGDGIMALFGAPLAHEDHAVRACYAAL
                     RMQDA"
BASE COUNT          427 a         1179 c         1143 g          455 t
ORIGIN      
        1 ggcctggcgc ccgtccttga gccgcacggt gagccgcgtc cacatgtggc gctcgagatc
       61 gcccgggatg tccggatcca cgaccacgcg aacgcgctcc atcaggcgcc ggatcgcagg
      121 atcctgcgcg cggtcgtcct ggaaggtggc catgcccacc cggccatcgg ccagcgcggc
      181 cgcggccgag aactcggccg agaacttcgc ttcgagcccg gtgcgcgggc gcgagtggat
      241 caggatgcgc gggacgcccg cgcacacgcc caccgtgagc tcctccacgt cgtccgcgcg
      301 gatgccctgg acccggcgca gctcgagcgc gccgtcgatg atggagtgcg tgcaggcgca
      361 ggaggggtag ggcttcaccg cgaccccggt ggtcaggatc ttccacggcg cgcccagggt
      421 gacgagcggc tcgagcgccc gctttcccgc gcccagtacc gcgaagaacc cctgcggtcc
      481 ctcgatcgcg tgctccgacg cggtccagcc ctcgcgcgcc atgagggcgg agagcacgcc
      541 actccgcgcc gcgtgcccgg cgtggaaggg cttggtcatg gtgccgaagt tctccttgag
      601 cccggaggac tgcgatgccg ccaccgcgag ggcgtgacgc gtctggaccg cgtcgaggcc
      661 gaggagccgc gccgccgccg cggccgcccc cagggtcccg agcgtacagg tggcgtgcca
      721 gccgtgctcg tagtgcgcgg ggttgatgac ctcggcgagc gtggtctcca cctcgaagcc
      781 caggaggaag gcgtggagca ccgcccggcc gtcggccagc gccagctcgc ccgcggccag
      841 ggacgcggcc agcaccggcg cggagggatg gcccatcaac gcgaagttgg tgtcgtcgaa
      901 gtcgagcgcg tgggccgcgg tgccgttgca gagcgcggcc cataccgcgc cggtccgccg
      961 gccggtgccc accaccgtgg ccagcggcag gccgccttcg gcctgcgcga cctgctggag
     1021 gatgcgcgcg gccggctcga tggagccggc cagcatcacg ccgaggcagt cgaggatggc
     1081 ccggcgggcg ccatccagtg ccgcctccgg gcagtcttcc aggcgactct tggtgacgaa
     1141 ctcggccagc atcgacgtgg tgttcatggc gggagcctac cacagaggcc ccgatcgcac
     1201 tgtcccgcgc ccgctcggcc ccgtgcctgg caccgccgag gccggccctg tatgatcggg
     1261 cctcgcccat gcgccggctc gcctggtgtc tcaccgtcgt cgcgatcatc ctggccgccg
     1321 ctcccgcccg ggcccagctc ttcgtcgcct cgcgccccga tccgcccttc accatcggcc
     1381 cgctgatggt ccgggccacc gtgaccgagg gccccgggcc ggtgccggtg gtggtgggct
     1441 tcagcctgca gctcgcgccg aaccgcaccc ccgccgacgt ggcccaggac gtcttcctgc
     1501 tctggccggg cgaggtcgtc aacgcggcgc ccgaccggaa ggccgacgcg accctggccc
     1561 gctacgtcac cgaccagggc ttcgagatca ccggggaggg ccacgtgaag ctgatggccc
     1621 gcaacctcgg cgacgcgggc accgtggagg ccctgccggc cggagcgccc ttcgtgacct
     1681 tcgtgcagac cgggccgctc ggcctgagcg ggccggcgac cttcgtccgc atcccgtggt
     1741 cgccccggct cgccgaccgg agctggctca tggagctgac cctcgacgcg gccgggctca
     1801 tcaagccggt gaaggtgggc tgggcggagc gcctcgtccg cggcacccac taccgggtgg
     1861 cggtcgcgtt ccacgaggtg cgggatcgtc cgctctttcc gatgtacttc gcgcaccgcg
     1921 accgggtggt gcggctggcc gacgcaccgg ccgagctggt cgtgcaattt ccccagtccg
     1981 agcgcctcaa gatcgacgac gtgtacccgc cgacggctat cagacggctg agcgagacgc
     2041 tggagaccac cgaggtggtg tcgctgttcc tcgaccgctc ggacgggatc accccgcagc
     2101 agctcgccgt gcagttcggc tacttctcgc gcacccagac cgcactgctg gtggccgccc
     2161 cgctgctcat cttcgcactg gggcaggcga tgggtcccct cctcggccgt gggctcctgc
     2221 gggtgatcga cgtggtgagc gcgcgcgtgc agctcggcgg gtggcgcgtc ggcggacggg
     2281 accgggaaca aggcgtgatc ctgccccgcg agacgctcga gcgcatcgcg cccggcaaga
     2341 cgacccgcga ggaggtgctg cggctctgcg gcaccgagat ggagcgtcag gatcagctct
     2401 ccggccgcac caccctgatc taccgcggcc gccgactcgt accggaggcc caccggatct
     2461 tcggctggct ctcgacggtg cgccgctggg acgtggagcg gcacgaggtg cggatcgagc
     2521 tggacggcga cctggtccgc gacgtccagg ccgaggtccg gcgctaccgc ctgggcgcgg
     2581 aggaggcccg ctgagcccgg tttgcgggcg cggaaccgtc cccccggcgg tccttgaccc
     2641 cccctgttcc gaggggctac tcttgttgga ctatccatct ggaccgctcc gaccccgagg
     2701 agggccgagg tgaagtgtcc ccgctgccag tcccagaacc gcgagggcgt tcggttctgt
     2761 gaggagtgcg gtggccgcct cgtcaccagc tgcgcgagct gtggcgccga gctgctcccg
     2821 gacaagaagt tctgcggctc ctgcggctcg ccggccaccc ccgagccggt agagcgccag
     2881 ccttcgaccc aggcctacac ccccaagcac ctcgcggaga agatcctgac ctcgcgccat
     2941 gccctcgagg gcgagcgcaa gcaggtcacg gtgctgttcg ccgatctcaa gggctcgatg
     3001 gagctcctcg ccgatcggga tcccgaggag gcgcgccgtc tgctcgatcc ggtactcgag
     3061 cggatgatgg aggcggtgca ccgctacgaa ggcaccgtga accaggtgat gggcgacggg
     3121 atcatggcgc tcttcggcgc tccgctcgcc cacgaggatc atgccgtccg ggcctgctat
     3181 gcggcgctcc gcatgcagga cgcc
//
LOCUS       DATMCV010000481         7775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_360348, whole genome shotgun sequence.
ACCESSION   DATMCV010000481 DATMCV010000000
VERSION     DATMCV010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360348"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VNU03_11100"
     CDS             <1..501
                     /locus_tag="VNU03_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HWP75057.1"
                     /translation="QRSGVSVRVTICNYENLLSSALKRAIRLGETVAAPRVSDLGAIA
                     ASTGGKIEMESLGDATEDKVMGKIMQRAVLNVFNRTFTSGELDEVVGAFQDGLKIEVS
                     DAMPAELYLRHLGQVRPLQAAVRKLGAQDPASVAAAVEFVLEGLHLSKKLNKDVQAGQ
                     SRYRGA"
     gene            509..2518
                     /locus_tag="VNU03_11105"
     CDS             509..2518
                     /locus_tag="VNU03_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWP75058.1"
                     /translation="MSPLSWYSRWDGSQRLDDLDADQLLEAMSDDLLSDGDPWSALRR
                     LFQRGAKNPDGANLPGLQDLLNRLRRQRQQQLDRYDLSSVLDDIKQKLDTIMKTEREG
                     IERRVADAQERAQKGEAPESFKQGMEQAAAEHRKTLDEMPESPAGRIQGLQNYEFMDP
                     DAHRLFWELMKGLQQQMLQPFLSNMQKALGNMSPQDLERMREMLRDLNRMLQDRAEGR
                     EPDFDAFKQKWGDHFPGAENLDQLMEQIAQQSGQMQSMLASMSPGQRRQLQDMMQSLF
                     MQDERLEGELRQLGMNLSQLMPPPDGRRYNFRGDDELSMKQAMELMDELRQLDDLERQ
                     IQKVRDPNDLEKIDPEQVEQLLGEEAKRDLERLREMTKKLEEAGYLERKGDRLELTAR
                     AIRKIGDKALRDIFGHLKRDRFGGHALERRGAGGDRTDQSKPYEFGDPFLLELRETLM
                     NAVERNGAGIPVRLAPADFEVARTETQTHAATVLMLDMSRSMIFNGCFLPAKKVALAL
                     NALIRGQFPRDSLYIVGFSLYAREFSAAQLPHLAPGEWSVGTNMHAGFLLARQLLARH
                     KGGNKQVIMITDGEPTAHMEGSEPEFAYPPTRRTLQETLKEVQRCTREDITINTFMLE
                     QSHALTAFVEQMTRINRGRAFFATSDRLGEYVLVDFVRSKRRAVS"
     gene            2530..3168
                     /locus_tag="VNU03_11110"
     CDS             2530..3168
                     /locus_tag="VNU03_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWP75059.1"
                     /translation="MPDAASVVLLVRHGLTPTTGIKLPGRAPGLHLSDEGRRQAEAAA
                     ERIGKVARIAAVYCSPITRARETAQPIGRAVKRALRIERDLADLDIGEWTGMSLKQAA
                     RRPEWETVQRNPSSFRFPGGESFPEMQTRMTSALGRIVARHPGQIVVAVSHADPIKAA
                     VAQALGTPLDLFQRIMIAPSSITVVAYRRGGPSVLTVNSLSGDLVWLGGGKP"
     gene            3165..3707
                     /locus_tag="VNU03_11115"
     CDS             3165..3707
                     /locus_tag="VNU03_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HWP75060.1"
                     /translation="MSDNPSFDLDAPDHFTAGAIGPPGQRVFYLQSREGGRVITLKVE
                     KEHVRALAEYLGGLLARVKGAPAQVQGGAELLEPVQPVWDVGSIAVGYDEPRDRVVVE
                     ASELLEEAEEEAKGEEAGEESGPQPAMARFRITRAQAAAFVDRANELMKGGRPSCPIC
                     SRPMDPEGHVCPRSNGHVTH"
     gene            3710..4423
                     /locus_tag="VNU03_11120"
     CDS             3710..4423
                     /locus_tag="VNU03_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HWP75061.1"
                     /translation="MPGRADPTPALDLLTRGEVTVKGRLPRSSNATFLVECCLDGQTA
                     LAVYKPERGERPLWDFPPGLYRREIAAYLLSEALGWDLVPLTVERDGPLGDGSLQLFV
                     PADFRQHYFTLLEQEEHREALRRICVFDLVANNADRKSGHCLHVPEDRIYAIDHGLTF
                     HAEPKLRTVIWDFGEEPITPALLEDLRRLLAAGLPPALAELLDADEQRALKRRARGLL
                     RSGHFPVDESGLRYPWPLI"
     gene            complement(4420..4677)
                     /locus_tag="VNU03_11125"
     CDS             complement(4420..4677)
                     /locus_tag="VNU03_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75062.1"
                     /translation="MSDIPAETPRIARAYCPACEPDADTLLEILEVQWCAIHMPLREG
                     LDDARVMSDAMLTGSAEAGGDENRRWCDVIHGNPTRHGRHG"
     gene            complement(4793..5701)
                     /locus_tag="VNU03_11130"
     CDS             complement(4793..5701)
                     /locus_tag="VNU03_11130"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWP75063.1"
                     /translation="MAHPTTGGPAGWGSPVAAPPDPSAAAFAWSFAPELILPLAALAG
                     LYALGWWRLARRHPRARPPWRLGAGIAGWLVLAAALLTPVAALGHFLFAAHMTQHMLL
                     MAVAAPLLLLADPLPAVLWGLPRPLRRPLGRLLVGGALLRTAWERLTSVPAAAVVYAL
                     VLWGWHHPRAYEAALGGEWVHHLEHVAFFGAAVVFWWPVIGPAPRARRAPSYGLRIAH
                     VVGAGLHGSALAMLLAWSPRVLYATYAAAPRVTALQPLEDQALGGVIMWAGASAADML
                     AVLALVWGFLAALERPAGASVELGVK"
     gene            complement(5934..6125)
                     /locus_tag="VNU03_11135"
     CDS             complement(5934..6125)
                     /locus_tag="VNU03_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75064.1"
                     /translation="MSDEPEHHPRYGEVEQYARGEIRAYHGIVNVWLLFVYAALAVWG
                     VYYLFKYWGGLGPGLGAGQ"
     gene            complement(6115..6276)
                     /gene="ccoS"
                     /locus_tag="VNU03_11140"
     CDS             complement(6115..6276)
                     /gene="ccoS"
                     /locus_tag="VNU03_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HWP75065.1"
                     /translation="MLEYLTFGAFLVALLMSLAALMAFVWGATSGAFTDIEGVKHRVL
                     TLEGIDHER"
     gene            complement(6281..>7775)
                     /locus_tag="VNU03_11145"
     CDS             complement(6281..>7775)
                     /locus_tag="VNU03_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HWP75066.1"
                     /translation="KWVRYDASDYTPLEQVGRAVYIREGCWYCHSQYVRPVTGEDLRW
                     GPVSEAGEYAYDRPHLFSTRRIGPDLTRVGLKYGDDWHYAHHWDPRLVVPESIMPSFR
                     WLYTQLRVPLKKEGDALVLEASPALGRWFTMKGERPIRLYPNETGLTFVPPGPEGAGP
                     LDGNPVIDLTGFKEKPPALTAVTLVRPTQEMVGLVRYIQKLGTNRGAWREVFEPQNVG
                     ISGMTIPSGHDLVALGREVYQSRCVGCHGVKGDGNGVAATFLQPRPRDFTVGVFKFRT
                     TPSGSLPTDGDLYRTVTRGVRWTAMPTWHELPDKERLAAVVFIKTFSTRWKDETPEPT
                     LQIGDPPKATPELLARGKDLYVKAKCFQCHGPGGKGDGPSADEMKDLAGFPILPADFT
                     RGQFKGGSSVRDIFRTMSLGLDGTPMPSFADSMSEPERWAISYYVLSLSAWMDPLTGQ
                     RLELPAGARATLNSRETDAGHPRLAVDPHEPTQTAQPGRRLYPGIRE"
BASE COUNT         1254 a         2747 c         2763 g         1011 t
ORIGIN      
        1 cagcgctccg gggtctcggt gcgcgtcacc atctgcaact acgagaacct gctctcgagc
       61 gcgctcaagc gggcgatccg gctcggggag acggtggccg ccccgcgggt cagcgacctc
      121 ggcgccatcg ccgcctccac cggaggcaag atcgagatgg agagcctggg cgacgcgacc
      181 gaagacaagg tgatgggcaa gatcatgcag cgcgcggtcc tcaacgtgtt caaccgcacc
      241 ttcacctcgg gcgagctcga cgaggtggtg ggcgccttcc aggacggcct caagatcgag
      301 gtctcggacg cgatgcccgc cgagctgtac ctgcgccacc tcggccaggt gcgccccctg
      361 caggcggcgg tgcgcaagct cggagcgcag gacccggcca gcgtggccgc cgcggtcgag
      421 ttcgtgctgg agggcctgca cctcagcaag aagctcaaca aggacgtgca ggccggccag
      481 tcgcggtacc ggggggcgtg aggcgccggt gtccccgctg agctggtact cgcgctggga
      541 cggcagccag cgcctcgacg acctcgacgc cgatcagctg ctcgaggcga tgtccgacga
      601 tctgctgtcg gacggcgatc cgtggagcgc gctccggcgc ctcttccagc gtggggcgaa
      661 gaacccggac ggggccaacc tgcccgggct tcaggatctg ctcaatcgcc tgcggcggca
      721 gcgccagcag cagctcgacc gctacgacct ctcctcggtg ctcgacgaca tcaagcagaa
      781 gctcgacacc atcatgaaga ccgagcgcga gggcatcgag cggcgggtgg ccgacgccca
      841 ggagcgggcc cagaagggtg aggcccccga gtcgttcaag caggggatgg agcaggccgc
      901 cgccgagcac cggaagacgc tcgacgagat gccggagtca cccgccgggc gcatccaggg
      961 gctgcagaac tacgagttca tggatcccga cgcgcaccgc ctgttctggg agctgatgaa
     1021 gggactgcag cagcagatgc tccagccgtt cctgtcgaac atgcagaagg cgctcggcaa
     1081 catgagcccg caggacctcg agcggatgcg ggagatgctg cgcgatctca accgcatgct
     1141 ccaggaccgc gcggagggcc gcgagcccga cttcgacgcc ttcaagcaga agtggggcga
     1201 ccacttcccg ggggccgaga acctcgacca gctcatggag cagatcgccc agcagagcgg
     1261 ccagatgcaa tcgatgctcg cgagcatgtc gcccggccag cgccgccagc tccaggacat
     1321 gatgcagtcg ctcttcatgc aggacgagcg gctcgagggc gagctgcgac agctcggcat
     1381 gaacctctcc cagctcatgc cgcctcccga cgggcgccgc tacaacttcc gcggcgacga
     1441 cgagctctcc atgaagcagg cgatggagct gatggacgag ctgcggcagc tcgacgacct
     1501 cgagcggcag atccagaagg tgcgcgaccc caacgacctc gagaagatcg accccgagca
     1561 ggtcgagcag ctcctgggcg aggaggccaa gcgcgatctc gagcgtctgc gcgagatgac
     1621 caagaagctg gaagaagccg gctacctcga gagaaaaggc gaccgcctcg agctgaccgc
     1681 gcgggccatc cgcaagatcg gcgacaaggc cctgcgcgac atcttcgggc acctcaagcg
     1741 cgaccggttc ggcggccacg cgctggagcg gcgcggcgcg ggcggcgacc gcaccgacca
     1801 gtccaagccc tacgagttcg gcgacccgtt cctgctcgag ctgcgcgaga ccctgatgaa
     1861 cgcggtggag cgcaacgggg cggggatccc ggtgcgcctc gctcccgccg acttcgaggt
     1921 ggcccgcacc gagacccaga cccacgccgc caccgtgctc atgctcgaca tgagccgctc
     1981 gatgatcttc aacggctgct tcctgcccgc caagaaggtc gccctcgcgc tcaacgcgct
     2041 gatccgggga cagttcccgc gcgacagcct gtacatcgtg ggcttctcgc tctacgcgcg
     2101 cgagttcagc gcggcccagc tcccccacct ggcccccggt gagtggagcg tgggcaccaa
     2161 catgcacgcc ggcttcctgc tcgcccgcca gctcctggcc cggcacaagg gcggcaacaa
     2221 gcaggtgatc atgatcaccg acggcgagcc caccgcccac atggagggga gcgagcccga
     2281 gttcgcctac ccacccaccc ggcggaccct gcaggagacg ctcaaggaag tgcagcgctg
     2341 cacgcgggaa gacatcacca tcaacacctt catgctggag cagagccacg ccctgaccgc
     2401 cttcgtcgag cagatgacgc gcatcaaccg cgggcgtgcc ttcttcgcaa cgtccgaccg
     2461 cctcggcgag tacgtgctcg tcgacttcgt ccgctccaag cggcgggccg tctcctagcc
     2521 cccgatcgga tgccggacgc cgcgtcggtc gtgctgctcg tgcgtcacgg gctgacgccc
     2581 accaccggga tcaagctgcc cggccgggcc ccgggcctcc acctctccga cgaggggcgg
     2641 cgccaggcgg aggcggccgc ggagcggatc ggcaaggtcg ccaggatcgc cgcggtgtac
     2701 tgctcgccga tcacccgcgc gcgcgagacc gcccagccga tcggccgggc ggtgaagcgg
     2761 gcgttgcgca tcgagcgcga cctcgccgac ctcgacatcg gagagtggac cggcatgagc
     2821 ctcaagcagg cggcccggcg accggagtgg gagacggtac agcggaaccc gagtagcttc
     2881 cgcttcccgg gcggcgagtc cttcccggag atgcagaccc gcatgacctc cgcgctcggc
     2941 cggatcgtgg cccggcaccc aggccagatc gtggtggcgg tctcgcacgc cgaccccatc
     3001 aaggccgcgg tggcccaggc cctgggcacc ccgctcgatc tcttccagcg catcatgatc
     3061 gcgccctcgt cgatcacggt ggtggcctac cggcgcggcg ggcccagcgt gctcaccgtc
     3121 aactccctct ccggcgacct cgtatggctc gggggcggca agccatgagc gacaacccct
     3181 ccttcgacct cgacgcgccg gaccacttca ccgcgggggc gatcggcccg cccggccagc
     3241 gcgtgttcta cctccagtcg cgcgagggcg ggcgtgtcat caccctgaag gtggagaagg
     3301 agcacgtgcg cgcgctcgcc gagtacctgg gtggcctgct cgcccgcgtg aagggcgccc
     3361 ccgcgcaggt ccagggcgga gccgagctac tcgagccggt gcagccggtg tgggacgtgg
     3421 gctcgatcgc ggtcggctac gacgagccca gggaccgggt ggtggtcgag gcgagcgagc
     3481 tgctcgagga agcggaagag gaggccaagg gcgaggaagc cggggaggag agcggcccgc
     3541 agccggcgat ggcgcgcttc cggatcaccc gcgcgcaggc cgccgccttc gtcgaccggg
     3601 ccaacgagct catgaaaggc gggcggccga gctgcccgat ctgcagccgg cccatggatc
     3661 cggagggcca cgtgtgcccc cggtccaacg gccacgtgac ccactaggga tgccggggcg
     3721 ggccgacccg acccccgcgc tcgacctgct gacgcgcggc gaggtcaccg tgaaggggcg
     3781 cctgccccgc agcagcaacg ccaccttcct cgtcgagtgc tgcctcgacg gccagaccgc
     3841 gctggcggtc tacaagcccg agcgcggtga gcggccgctg tgggatttcc cccccgggct
     3901 ctaccggcgc gagatcgcgg cctacctgct ctcggaagcg ctcggctggg acctcgtccc
     3961 tctcacggtg gagcgggacg ggccgctggg agacggatcg ctccagctct tcgtgccggc
     4021 cgattttcgc cagcactact tcacgctgct ggagcaggaa gagcaccggg aggcgctccg
     4081 gcgcatctgc gtcttcgacc tggtggccaa caacgcggac cgcaagagcg gccactgcct
     4141 ccacgtgccg gaggaccgga tctacgcgat cgatcacggc ctcacgtttc acgccgagcc
     4201 gaagctccgc accgtgatct gggatttcgg cgaggagccg atcactcccg cgctgctgga
     4261 ggatctgcgg cggttgctcg ccgcgggcct gccgcccgcg ctcgccgaat tgctcgacgc
     4321 cgacgagcag cgcgcgctca agcggcgcgc gcgggggctg ctgcgctcgg gccacttccc
     4381 ggtggacgag agcggcctgc gctacccctg gccgctgatc tagccgtggc ggccgtggcg
     4441 cgtcgggttc ccgtggatca cgtcgcacca gcggcgattc tcgtcgcccc ccgcctcggc
     4501 gctgccggtc agcatcgcgt ccgacatgac ccgcgcatcg tccagcccct cccgcagggg
     4561 catgtggatg gcgcaccact gcacctccag gatttccagc agggtatcgg cgtcgggctc
     4621 gcaggcgggg cagtatgcgc gggcaatacg gggcgtttca gccggaatgt cgctcaaggc
     4681 aggccaccac ggtgggagac ggctggactt cctcgcgagt gtcgagcgat cgcaacctcc
     4741 tgtccggaat gccgaaccgg gctcgagcct cacgaccatt ctacacacga gattatttca
     4801 ctcctaattc taccgacgcc ccggccggcc gctccagcgc cgcgaggaac ccccacacga
     4861 gggccagcac cgcgagcatg tccgccgcgg acgccccggc ccacatgatg accccgccga
     4921 gcgcctggtc ctcgagcggc tggagcgcgg tcacccgggg cgccgccgcg tacgtcgcgt
     4981 agagcacgcg cgggctccac gccagcagca tcgcgagggc cgagccgtgg agcccggcgc
     5041 cgaccacgtg cgcgattcgc aggccgtacg agggcgcgcg ccgggcacgc ggtgcggggc
     5101 cgatcaccgg ccaccagaac acgaccgcgg cgccgaagaa ggccacgtgc tcgaggtggt
     5161 gcacccactc cccgccgagc gccgcctcgt aggcgcgcgg gtgatgccac ccccagagca
     5221 ccagggcgta cacgaccgcg gccgccggca ccgaggtgag ccgctcccag gcggtgcgca
     5281 ggagggctcc accgacgagc agccggccca ggggacggcg cagtggccgc gggagacccc
     5341 agagcaccgc cggcagtgga tcggccagca gcaggagcgg cgcggccacc gccatcagca
     5401 gcatgtgctg ggtcatgtgc gcggcgaaca ggaagtggcc gagggcggcc accggggtga
     5461 gcagggcggc ggcgagcacg agccagccgg cgatgccggc gccgagccgc cacgggggcc
     5521 gggcccgcgg gtgccgccgc gcgagccgcc accagccgag cgcgtacagg cccgccagcg
     5581 ccgcgagcgg caggatcagc tcgggcgcga agctccaggc gaaggcggca gcggagggat
     5641 ccggcggcgc cgcgaccggt gagccccagc ccgcgggacc gccggtggtc ggatgcgcca
     5701 tccgcctact tgcggaggac caggacgatg acccagacga agaagaggac cgtcccggcg
     5761 aggaagaggc cgaaagcgat ggtctcgttg gtgctcatgg gcgggaggcc tccatcagaa
     5821 gagcgacacg aggccggaca gcgcgttgac caggatgtcg agaccgagca cgagcaggtt
     5881 caggaccgcc agcgcgagca cgatgcgttc ggcgagcatg ggatctccgc ctcctactgg
     5941 cccgcgccca ggcccgggcc gagcccgccc cagtacttga agaggtagta gacgccccag
     6001 accgcgagcg cggcgtacac gaagaggagc cagacgttca cgatgccgtg gtaggcgcgg
     6061 atctccccgc gcgcgtactg ctccacctcg ccatagcgcg gatggtgctc gggctcatcg
     6121 ctcatgatcg atcccctcca gcgtcagcac gcgatgcttc acgccctcga tgtcggtgaa
     6181 cgccccgctc gtggcccccc agacgaaggc catgagcgcg gccaggctca tcagcagcgc
     6241 gaccaggaag gcaccgaagg tcaggtattc gagcatgggg tcactcgcgg atgccggggt
     6301 agagacggcg gcctggctgc gccgtctggg tgggctcgtg gggatcgacg gccagccgcg
     6361 ggtggccggc gtcggtctcg cgcgagttga gcgtggcgcg cgcgcccgcg ggcagctcca
     6421 ggcgctggcc ggtgagcggg tccatccacg cggagagcga gagcacgtag taggagatcg
     6481 cccagcgctc cggctcgctc atcgagtcgg cgaacgacgg catcggggtg ccgtcgagcc
     6541 cgagcgacat ggtgcggaag atgtcgcgca cgctcgagcc gcccttgaac tggccgcggg
     6601 tgaagtcggc cgggaggatc gggaagccgg ccaggtcctt catctcgtcg gccgacggcc
     6661 cgtcgccctt gccgcccggg ccgtggcact ggaagcactt ggcctttacg tagaggtcct
     6721 tgccgcgggc caggagctcc ggggtggcct tgggcgggtc gccgatctgg agcgtgggct
     6781 cgggtgtctc gtccttccac cgcgtggaga aggtcttgat gaagaccacc gccgcgagcc
     6841 gctccttgtc cggcagctcg tgccaggtgg gcatcgcggt ccagcgcact ccgcgagtga
     6901 cggtccggta gaggtcgccg tcggtgggca gcgacccgga cggcgtcgtg cggaacttga
     6961 agacgccgac cgtgaagtcg cgcggccgcg gctggaggaa cgtggcggcc accccgttac
     7021 cgtcgccctt gacgccgtga cagcccacgc agcggctctg gtagacctcg cggccgaggg
     7081 cgaccagatc gtgcccgctc gggatggtca tgccggagat cccgacgttc tgcggctcga
     7141 agacctcccg ccaggcgccg cggttggtgc cgagcttctg gatgtagcgg accagcccga
     7201 ccatttcctg ggtgggccgc acgagggtca ccgcggtcag ggcgggcggc ttctccttga
     7261 aaccggtgag gtcgatcacc gggttgccgt cgaggggccc ggctccctcg gggccgggcg
     7321 gcacgaaggt cagcccggtc tcgttcgggt agaggcggat cggccgctcg cccttcatgg
     7381 tgaaccaccg gccgagcgcg ggcgaggcct ccaggaccag cgcgtcgcct tccttcttga
     7441 gcgggacgcg gagctgggtg tagagccacc ggaaactcgg catgatcgac tcgggcacca
     7501 ccagccgcgg gtcccagtgg tgcgcgtagt gccagtcgtc gccgtacttg aggccgacgc
     7561 gggtgaggtc ggggccgatc cggcgggtcg agaacaggtg cggccggtcg taggcgtact
     7621 cgccggcctc cgagaccggc ccccagcgca ggtcctcgcc ggtcaccggc cgcacgtact
     7681 ggctgtggca gtaccagcag ccctcgcgga tgtagaccgc gcggcccacc tgctcgagcg
     7741 gggtgtagtc gctcgcgtcg tagcgcaccc acttc
//
LOCUS       DATMCV010000482         3243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_557595, whole genome shotgun sequence.
ACCESSION   DATMCV010000482 DATMCV010000000
VERSION     DATMCV010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557595"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VNU03_11150"
     CDS             <1..166
                     /locus_tag="VNU03_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWP75067.1"
                     /translation="CQAGQIMSATALLKRNRKPTDAEIDEAMSGNICRCGTYVRIRQA
                     IKQAALGAAR"
     gene            163..2478
                     /locus_tag="VNU03_11155"
     CDS             163..2478
                     /locus_tag="VNU03_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP75068.1"
                     /translation="MSPLGTEGATDRPIVIANVSRRRFIQGVSAMGGLVLAAGFSSAA
                     NAADPPKYGRDGMPNGWSDDPLVFVAIGDDGIVSIVCHRSEMGQGVRTGMPMIVADEL
                     EADWKRVRVVQATGDEKRFGNQDTDGSRSTRHFFDPMRRCGAAARTMLEAAAAAKWGV
                     PVSEVEAKNHEVVHAKSGRRAGYGSLAKAAATQTVPARDTVRLKDPSKFRYIGKGNLK
                     LLDGPDITTGKAQYGIDTRLPGMVYAVVARPPVYGGKMVSYDAAETLKVPGVLKVVEI
                     EPSAAPAQFNPLGGVAVVAKNTWAAIQGRRALKVVWDDGANGSYDSTAFKAQMEESAR
                     KPGKVVRDNGNVDTALAGAAKRLEAEYYIPHLAHAPMEPPAATVRIVNGKAEAWACVQ
                     SPQAARDLVAKRLGIPADDVTVNVTLLGGGFGRKSKPDYVVEAAVVSKAMDGKPVKVT
                     WTREDDLMHTYFHTVSVEHLEAGLDAQGKPVAWMHRSVAPTIFSTFVAGAKNEADLEL
                     GMGVINVPFAIPNVRIENPEAEAHTRIGWFRSVSNIPHAFAVQSFVAEMAAAAGRDQK
                     DYLLEVIGPARRVSPETLGDKWNHGESPERYPVDTGRLRRVVETVAREAGWGRKLPKG
                     RGLGIAAHYSFVSYVASVVEVAVDDQGVVSVPRIDIAVDCGATVNPDRVRAQMQGAAI
                     MGLAVATLGEMSFKGGRAEQQNFDTYQVTRMAESPRDIRVHVLPGDYAQPMGGVGEPG
                     VPPVPPALCNAIFAATGKRIRRLPIRDQLKA"
     gene            complement(2536..>3243)
                     /locus_tag="VNU03_11160"
     CDS             complement(2536..>3243)
                     /locus_tag="VNU03_11160"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HWP75069.1"
                     /translation="ADLLPRHRIFLSVGHDEYWSWAMREHVEAARDGGVHLAFLGANA
                     CFWQIRFEPGASGAPDRTVVAYKDAVASDPVAADPGGARARLATGRWRDPPTALPEAA
                     LIGIMYVADPVDGDVVVDDADHWVFAGTGLRRGSVLPGLLGYEVDAVAEGSPSGVRRL
                     AHSPFARPSGETGYADMTIHETAGGALVFATGSMYWNWGLDGYNAPRWHGDRVSAAAQ
                     RITRNVLDGMLARGPRK"
BASE COUNT          493 a         1141 c         1185 g          424 t
ORIGIN      
        1 ctgccaggcg ggccagatca tgtccgcgac cgcattgctc aagcgcaacc gcaagcccac
       61 cgacgccgag atcgacgagg ccatgagcgg caacatctgt cgctgcggga cctacgtccg
      121 gatccgccag gccatcaagc aggcggctct gggagcggcc cgatgagccc gctcgggacg
      181 gaaggcgcga cggaccgtcc gatcgtgatc gccaacgtga gccggcggcg gttcatccag
      241 ggtgtgtccg cgatgggcgg cctcgtcctg gccgcgggct tctcttccgc cgccaatgcg
      301 gccgacccgc cgaagtacgg ccgggacggc atgcccaacg gctggagcga cgacccgctc
      361 gtgttcgtgg ccatcgggga cgacggcatc gtctccatcg tctgtcaccg ctccgagatg
      421 gggcagggcg tccgcaccgg catgcccatg atcgtggccg acgagctcga ggccgactgg
      481 aagcgcgtcc gcgtggtgca ggccaccggg gacgagaagc gcttcggcaa ccaggacacc
      541 gacggatcgc ggagcacccg ccatttcttc gatcccatgc ggcgttgcgg tgcggcggct
      601 cggaccatgc tcgaggcggc ggccgccgcg aagtggggcg tgccggtctc cgaggtggag
      661 gcgaagaacc acgaggtcgt gcacgccaag tcgggccggc gggcgggata cggctcgctc
      721 gccaaggcgg ccgccaccca gaccgtcccg gcccgcgaca ccgtgcggct caaggacccg
      781 tccaagttcc ggtacatcgg caagggcaac ctgaagctgc tcgacggccc cgacatcacc
      841 accggcaagg cgcagtacgg catcgacacg cggctgcccg gcatggtcta cgcggtggtg
      901 gcgcggcccc cggtctacgg cggcaagatg gtgagctacg acgcggccga gaccctcaag
      961 gtccccggcg tgctcaaggt ggtcgagatc gagccgagcg cggcgccggc ccagttcaac
     1021 ccgctgggcg gggtcgcggt ggtggcgaag aacacctggg ccgccatcca gggccgccgg
     1081 gccctcaagg tggtctggga cgacggggcg aacggcagct acgactcgac ggccttcaag
     1141 gcccagatgg aggagtccgc gcgcaagccg ggcaaggtgg tccgcgacaa cggcaacgtc
     1201 gacaccgcgc tggccggcgc ggccaagcgg ctcgaggcgg agtactacat tcctcacctg
     1261 gcgcacgctc cgatggagcc cccggccgcg acggtgcgca tcgtcaacgg caaggcggag
     1321 gcgtgggcct gcgtgcagtc gccccaggcc gcgcgcgacc tggtggccaa gcgcctcggc
     1381 attccggccg acgacgtcac cgtgaacgtg accctgctgg gcggcgggtt cggccgcaag
     1441 tccaagcccg actacgtcgt cgaggccgcg gtggtctcga aggcgatgga cggcaagccg
     1501 gtcaaggtga cgtggacgcg cgaggacgat ctgatgcaca cctacttcca caccgtgtcg
     1561 gtcgagcacc tggaggcggg cctcgacgcg cagggcaagc cggtggcgtg gatgcaccgg
     1621 agcgtcgcgc cgaccatctt ctccacgttc gtggcggggg ccaagaacga ggccgacctc
     1681 gagctgggca tgggcgtcat caacgtgccg ttcgcgattc ccaacgtgcg catcgagaac
     1741 ccggaggcgg aggcgcatac gcgcatcggc tggttccgct cggtgtcgaa catcccgcac
     1801 gcgttcgcgg tgcagtcctt cgtcgccgag atggccgcgg cggcgggccg cgatcagaag
     1861 gactatctcc tggaagtcat cgggccggcc cgccgggtca gcccggagac cctgggcgac
     1921 aagtggaacc acggcgagtc ccccgagcgg tacccggtgg acaccggccg gctgcggcgc
     1981 gtggtggaga ccgtcgcgcg cgaggcgggc tgggggcgca agctgcccaa ggggcgcggg
     2041 ctcggcatcg ccgcgcatta cagcttcgtg agctacgtcg cctcggtggt cgaggtggcg
     2101 gtggacgatc agggcgtggt gagcgtgcca cgcatcgaca tcgcggtgga ctgcggcgcc
     2161 accgtcaacc ccgaccgggt gcgggcgcag atgcagggcg ccgcgatcat gggcctggcc
     2221 gtcgccaccc tgggcgagat gagcttcaag ggcgggcggg ccgagcagca gaacttcgac
     2281 acctatcagg tcacccgcat ggcggagtcc ccgcgcgaca tccgggtgca cgtcctgccc
     2341 ggcgactacg cccagccgat gggcggggtg ggtgagcccg gcgtgccgcc ggtgccgccg
     2401 gccctctgca acgccatctt cgcggcgacc gggaagcgga tccggcgcct gccgatccgc
     2461 gatcagctga aggcctgagg tcgcttcacc ggacgtagag ggcggggccg gggggcggtt
     2521 gctcctcggc cccgtttact ttcgtggccc tctcgcgagc atcccgtcca gtacgttccg
     2581 ggtgatccgc tgcgcggccg cgctcacgcg gtctccgtgc caccgcgggg cgttgtagcc
     2641 gtcgagcccc cagttccagt acatcgagcc ggtggcgaac acgagcgcgc ctccggccgt
     2701 ctcgtggatg gtcatgtcgg cgtacccggt ctcgccggac ggccgggcga agggcgagtg
     2761 cgccagccgg cgcacccccg acgggctccc ctccgccacc gcgtccacct cgtagcccag
     2821 gagcccgggc agcacgctgc cccggcggag cccggttccc gcgaagaccc agtgatccgc
     2881 gtcgtcgacg accacgtcgc cgtcgaccgg atcggccacg tacatgatgc cgatcagggc
     2941 cgcctccggc agagcggtgg gcgggtcgcg ccagcgaccg gtcgcgaggc gggcccgcgc
     3001 cccgcctgga tcggcggcga ccggatcgct ggccaccgca tccttgtacg ccaccaccgt
     3061 gcgatccggg gcgccgctcg cgcccggctc gaagcggatc tgccagaagc acgcgttggc
     3121 gcccaggaac gcgaggtgga cgcctccgtc ccgggccgcc tcgacgtgct cgcgcatggc
     3181 ccacgaccag tactcgtcgt ggccgaccga gaggaagatg cggtgccgcg gcagcaggtc
     3241 cgc
//
LOCUS       DATMCV010000483         4398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_688845, whole genome shotgun sequence.
ACCESSION   DATMCV010000483 DATMCV010000000
VERSION     DATMCV010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688845"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VNU03_11165"
     CDS             complement(<1..1327)
                     /locus_tag="VNU03_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP75070.1"
                     /translation="MTAPVPAGARHLSDPILLELVKNGLDAIVDEMAIALVRTAYSNN
                     LKNAMDMSCALCDVDGRLIAQGLTLPLHLGSIPDAMAQVRRKFGASLEPGDVFLLNDP
                     FEGGTHLPDFYIFKPIFVAGRLVGWSTSIGHQLDVGGKTAGGNGCDATEIFQEGLRIP
                     PLRLYAAGKPVEALFELIDRNVRVPRQVLGDVRSQVAACLTGERGYLKLGEQYGPERF
                     AACTTALLDQAERLARNAITAMPDGRYTFTDWIDDDGIDPDPIPITVAITVAGDRLIA
                     DFTGSAPQVKGAINSPLPFTKSAVYACVRHLVGGTPPNNEGYFRPIQVIAPPGTVVNP
                     VMPAAVAARGLTGFRMANAVFGALAQIAPDRVFACEMGGDTGVSFGGYDAERRPFVFL
                     EFLFGSWGGRPTKDGVDACSSSVVNFSNNPVEVIESEYPLLIERYGYLPD"
     gene            complement(1324..3417)
                     /locus_tag="VNU03_11170"
     CDS             complement(1324..3417)
                     /locus_tag="VNU03_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP75071.1"
                     /translation="MSYRLGVDVGGTFTDLVLVSPDGVARTCKVLSTTANYAEAIIAG
                     VRELTAQAAIAPPGIGEIIHGTTVATNAILERRGARAGLLTTEGFRDLLEIGRLRLSR
                     LYDLDFERPAPLVPRRWRREVRERMSHRGEVITPLDRDGAAAAIDRLLAEGVESIAVC
                     FLHAYANPAHEAQVGAMIRERAPHVALTLSSEILPEMREFERTSTAVTNAYVMPVMGR
                     YLASLEGELRRLEVRAPLLIMQSNGGVMTAEGGRRRPVHVIESGPAAGVIATAALARA
                     IGAPNALSIDMGGTTAKASIIEGYEIKRTGEFEIGGSMSQGSRLNKGGGFLLRVPAID
                     IAEVGAGGGSIVHVDGAGALHVGPRSAGAVPGPVCYDQGGAEVTLTDANVTLGYLHPE
                     RLPSGLRLDAGKARRALAEQIAAPLGIDVADAAHGVYLLGCAGMARAVRAVTIERGRD
                     PRDFTLVAFGGNGPLFAAEMARSLEIGTILVPPAPGVFSALGLLEAEVEHHLVRTFLR
                     SLFGTKPSEIAAAVGELEREAEALLRAEGSRAQVEIARSIDLKYQGQSFELTVPLPPA
                     WTDDALRTLAAEFGREHERTYGHQAEGDPIHIVNLRLTARVLRPADRAAIRLQEDRAG
                     RGGDRRAYFGPTHGTIATPVVTRAALDATPRPGPLLVDEYDATTLVPPGCAARLDTHG
                     NIVITTAVTTTGDPA"
     gene            complement(3414..4340)
                     /locus_tag="VNU03_11175"
     CDS             complement(3414..4340)
                     /locus_tag="VNU03_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75072.1"
                     /translation="MKTRARLTLGAVGVAALLPVLLPHPFVLTIATQAAIWALLAASW
                     DLLSGYTGQISFGHAGFFALGAYGAAVATKHADLSPWLGLLVGAGVAAAVGLLTGFPA
                     LRLRGHYLALVTLGLAEIIRLIAQNWLEVTGGPFGIHDFRSLPGLPAGALLHRQAMYL
                     VVVAIVVVSVAVMYQVCERTSAGQAFRAIREDEVLAQTLGIDTTRYKLLAFSLSSAFA
                     GLAGGLYAYYVQLVSPVIAGAATTSLVIGMVVFGGLGTLWGPVLGALLLYGLYEGLRF
                     VGVVYNLVAVGFVIMVFVIFFPRGLAGLGRSA"
BASE COUNT          634 a         1510 c         1683 g          571 t
ORIGIN      
        1 tgtcggggag gtacccgtag cgctcgatca ggagcgggta ctcggactcg atgacctcga
       61 ccgggttgtt ggagaagttc accaccgagc tgctgcatgc gtcgaccccg tctttggttg
      121 gccggccgcc ccacgagccg aagaggaact cgaggaagac gaagggccgg cgctcggcgt
      181 cgtagccgcc gaagctcacc ccggtgtcgc cgcccatctc gcacgcgaag acccggtcgg
      241 gggcgatctg ggccagcgcg ccgaagaccg cgttggccat ccggaagccg gtgaggccgc
      301 gggccgccac cgcggccggc atgaccgggt tgaccacggt gccgggcggc gcgatcacct
      361 ggatcgggcg gaagtagccc tcgttgttcg gcggggtgcc gccgaccagg tgccgcacgc
      421 acgcgtagac cgcggacttg gtgaagggca gcggcgagtt gatggccccc ttcacctggg
      481 gggcggagcc ggtgaagtcg gcgatcaggc gatcgcccgc gaccgtgatg gccaccgtga
      541 tcgggatcgg gtcgggatcg atcccgtcgt cgtcgatcca gtcggtgaag gtgtagcgcc
      601 cgtccggcat ggcggtgatg gcgttgcggg cgaggcgctc ggcctggtcg agcagcgcgg
      661 tggtgcaggc cgcgaagcgc tcggggccgt actgctcgcc cagcttgagg tagccgcgct
      721 cgccggtgag gcaggcggcc acctgcgagc gcacgtcgcc cagcacctgg cgcggcacgc
      781 gcacgttgcg gtcgatcagc tcgaacagcg cctccaccgg cttgcccgcc gcgtagagcc
      841 ggagcggggg gatgcgcagg ccctcctgga agatctcggt cgcgtcgcac ccgttgccgc
      901 ccgcggtctt gccgcccacg tcgagttggt ggccgatgct ggtggaccag cccaccagcc
      961 gcccggccac gaagatgggc ttgaagatgt agaagtcggg caggtgcgtc ccgccctcga
     1021 acgggtcgtt caggaggaac acgtcaccgg gctcgaggct cgcgccgaac ttgcgccgga
     1081 cctgcgccat cgcgtccggg atggagccca ggtgcagcgg cagggtcagg ccttgggcga
     1141 tcaggcggcc gtcgacgtcg cagagggcgc acgacatgtc catcgcgttc ttgaggttgt
     1201 tcgagtaggc ggtccgcacg agcgcgatcg ccatctcgtc cacgatggcg tcgaggccgt
     1261 tcttgaccag ctcgaggagg atcgggtcgc tcaggtggcg ggcgccggcg ggcacggggg
     1321 cggtcatgcg ggatctccgg tagtagtcac cgcagtagta atcacgatgt tgccgtgggt
     1381 gtcgagcctg gccgcgcagc ccggcggcac cagggtggtg gcgtcgtact cgtcgaccag
     1441 cagcgggccc ggccggggcg tggcgtcgag cgcggcgcgg gtgacgaccg gggtggcgat
     1501 cgtcccgtgc gtcgggccga agtaggcgcg ccggtcgccg ccgcgtccgg cgcggtcctc
     1561 ctggaggcgg atcgccgcgc gatccgccgg ccgcagcacg cgcgcggtca ggcgcaggtt
     1621 cacgatgtgg atgggatcgc cctccgcctg gtggccgtag gtgcgctcgt gctcgcgccc
     1681 gaactcggcg gccagggtcc ggagcgcgtc gtcggtccac gcgggcggca gcggcaccgt
     1741 gagctcgaag gactgcccct ggtacttgag atcgatagac cgcgcgatct ccacctgggc
     1801 ccgactgccc tcggcgcgga gcagggcctc ggcctcgcgc tccagctccc ccaccgcggc
     1861 cgcgatctcc gacggcttcg tgccgaagag cgagcgcagg aacgtgcgga ccaggtggtg
     1921 ctcgacctcg gcctccagca gcccgagcgc gctgaagacg ccgggcgcgg gcgggactag
     1981 gatggtgccg atctcgagcg agcgcgccat ctcggctgcg aagagggggc cgttgccgcc
     2041 gaaggccacc agggtgaagt cgcggggatc gcggccgcgc tcgatggtga ccgcgcggac
     2101 cgcccgcgcc atgcccgcgc agccgagcag gtagacgccg tgcgcggcgt cggccacgtc
     2161 gatcccgagg ggcgccgcga tctgctcggc gagcgcgcgg cgggccttgc ccgcgtcgag
     2221 gcgcaggccg ctcggcaggc gctcggggtg caggtagccg agggtcacgt tggcgtcggt
     2281 cagcgtgacc tcggcgccgc cctggtcgta gcagaccggc cccggcaccg cgcccgcgct
     2341 gcgcgggccc acgtgcagcg cgcccgcccc gtccacgtgg acgatgctgc cgccgcccgc
     2401 gccgacctcc gcgatgtcga tggcgggcac ccgcagcagg aagccgccgc ccttgttgag
     2461 ccggctgccc tgggacatcg agccgccgat ctcgaactcg ccggtgcgct tgatctcgta
     2521 gccttcgatg atcgaggcct tcgcggtcgt tccccccatg tcgatgctga gcgcgttggg
     2581 ggcgccgatg gcgcgggcca gcgccgcggt ggcgatgacg ccggcggcgg ggcccgactc
     2641 gatcacgtgc accgggcggc ggcgcccgcc ctcggcggtc atgacgccgc cgttggactg
     2701 catgatgagc agcggggccc gaacctccag gcggcgcagc tcgccttcga gcgacgcgag
     2761 gtagcggccc atcaccggca tcacgtaggc gttggtgacc gcggtgctgg tgcgctcgaa
     2821 ctcgcgcatc tcgggcagga tctcggagga gagggtgagc gccacgtggg gcgcgcgctc
     2881 ccggatcatc gcgcccacct gggcctcgtg ggccgggttg gcgtaggcgt ggaggaagca
     2941 gaccgcgatc gactccacgc cctccgcgag caggcggtcg atggcggcgg ccgcgccgtc
     3001 ccggtcgagc ggggtgatca cctcgccgcg gtggctcatc cgctcgcgga cctcgcggcg
     3061 ccaccggcgg ggcaccagcg gggccgggcg ctcgaagtcg aggtcgtaga ggcgcgagag
     3121 gcgcaggcgg ccgatctcga gcaggtcgcg aaagccctcg gtggtcaaga ggccggcgcg
     3181 ggcgccgcga cgctccagga tcgcgttggt ggccaccgtg gtgccgtgga tgatctcgcc
     3241 gatcccgggc ggagcgatgg cggcctgcgc ggtcagctcc cgcacccccg cgatgatcgc
     3301 ctccgcgtag ttggcggtgg tcgacagcac cttgcaggtg cgcgcgacgc cgtcgggcga
     3361 caccaggacc aggtcggtga aggtgccgcc cacgtccact cccagccgat agctcatgcc
     3421 gatctcccga ggcccgccag cccgcgcgga aagaagatga cgaagaccat gatcacgaag
     3481 cccaccgcga ccaggttgta cacgacgccc acgaagcgca agccctcgta gaggccgtag
     3541 agcagcagcg cgccgagcac cgggccccac agggtaccga gtccgccgaa gaccaccatg
     3601 ccgatgacca gcgaggtggt ggccgcgccc gcgatcaccg ggctgaccag ctgcacgtag
     3661 tacgcgtaga gcccgccggc gaggcccgcg aaggccgagc tgaggctgaa cgcgagcagc
     3721 ttgtagcgcg tggtgtcgat cccgagggtc tgggcgagca cctcgtcctc gcggatggcc
     3781 cggaacgcct ggcccgcgct cgtgcgctcg cagacctggt acatgaccgc gaccgagacc
     3841 accacgatcg ccaccaccac cagatacatc gcctggcggt ggaggagcgc gccggcgggg
     3901 aggccgggca agctccggaa gtcgtggatg ccgaacgggc cgccggtcac ctcgagccag
     3961 ttctgggcga tgagccggat gatctcggcg agcccgagcg tgaccagggc caggtagtgg
     4021 ccgcgcagcc ggagggcggg gaagccggtc agcaggccga ccgcggcggc gaccccggcg
     4081 ccgacgagca gaccgagcca cggggagagg tcggcgtgct tggtggcgac ggcggctccg
     4141 taggccccga gcgcgaagaa cccggcgtgg ccgaacgaga tctggccggt gtagccgctc
     4201 agcaggtccc agctcgcggc gagcagcgcc cagatcgcgg cctgggtcgc gatggtgagg
     4261 acgaagggat gcggcagcag caccggcagc agtgcggcga cccccaccgc gccgagcgtg
     4321 agccgcgcgc gggtcttcat cgcgagacgg cccggccgag gaggccccgg gggcgcacca
     4381 ggagcatcac gatcatca
//
LOCUS       DATMCV010000484         4097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_962370, whole genome shotgun sequence.
ACCESSION   DATMCV010000484 DATMCV010000000
VERSION     DATMCV010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962370"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VNU03_11180"
     CDS             complement(<1..666)
                     /locus_tag="VNU03_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWP75073.1"
                     /translation="MTGYLAGLECTRCSDYYPGDTPRALCDCGSTLLVRYDLAVVRKA
                     VTPDDIARRPPTMWRYRELLPVRDEASIVTMGEGFTPMIPVPKAAREVGVGELWVKDE
                     GVNPTGTFKARGASASISRWKELGVTRFAQPTVGSGGHAWAAYGARAGVEIHTALPVS
                     CPDLGWKGSLTNGAHVYRVDGTVGDVFRMMGEASARHGWLLAGAGREPYRFEGKKTMG
                     LEIV"
     gene            complement(697..1545)
                     /locus_tag="VNU03_11185"
     CDS             complement(697..1545)
                     /locus_tag="VNU03_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75074.1"
                     /translation="MRPRVPPLLLLAAAPVVAAVICAVLAPQLAPYDPTRGDLSARLR
                     PPAWTAAGSARYLLGTDQLGRDILSRIIFGARVSLLVGVVSSLIGGTIGLTLGMLGGY
                     FRGRADSVVAKLIDVQLAFPFLLFAITVIAVLGPSLPNLLIILALSGWVTYARVVRGQ
                     TLSLRETDFIQAARGLGCPAWWILLRHVLPNVLSYALVIMTTEIGRLIVLESTLSFLG
                     LGVPPPTPSWGMALADGKEYLINGWWLATFPGLAIMMIVLSMNVLGDGARDLLEPRLR
                     GRARRG"
     gene            complement(1545..2465)
                     /locus_tag="VNU03_11190"
     CDS             complement(1545..2465)
                     /locus_tag="VNU03_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009112761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75075.1"
                     /translation="MRDFLLAKLGHTLIVLFCVLTLVFVVLHLTGDPVMMMLPSNASR
                     EEIDALTKSLGLDQPLIVQYGRFLARVAHGDFGVSLQHQQPAMGLVAERLPASMLLAG
                     SALLLALAVALPLGILAAAHRGSALDRVAIGLAALGQSAPIFWTGLMLMLVGSVILRL
                     LPTSGYGTWQHLILPALTLASYPMAAMARLVRSGMLEVLDADYVRTARAKGLPERGVV
                     LKHTLKNAAMPIVTVIGLQFGLLLGGAIVCEMIFAWPGVGRLMIFAIYNRDFPLVEAA
                     VFVMAMVFILANLLVDLCYAWLDPRVKLQA"
     gene            complement(2525..4054)
                     /locus_tag="VNU03_11195"
     CDS             complement(2525..4054)
                     /locus_tag="VNU03_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75076.1"
                     /translation="MQRTVLAVTLGLCLALLAIALPAAPAVGQDLVIAQGADITTLDP
                     TQATQIHNLNLFYNIYDALVTWDPKDIGKLVPELAVSWRSVDPLTWQFKLRQGVSFHN
                     GESFNADAVKFTVDRLITKGVHQVYGGFSTIERADVVDAYTINIVTSKPDPILVKRFA
                     GYGGQILPPQYIKQVDWKTFAIKPVGTGPYKFVEWVKDDRVVLEANPAYWRGAPKIKK
                     VVWRPIPDNFARVAALTRGEAQIITKVIPDHVSQVEKAGCCRVEHTLTNLVTVYLINA
                     QKGPLANTKVRQALNYAVDKDKIIKELYKGYAIPIGSGIPNTDFGYNAKIKPYPYDPA
                     RARQLLAEAGYPNGVDVDIQSGNGIHLNDRQLSEAVAVMLQDVGVRPKVGVLEPSQRS
                     QLLRTNTFPGLLLADPASTTYDTDGVIWRLRGPGGILHQAWPGNYEGTPFYKLMEEAR
                     VTLDVPKRLRNYQEAAQIWHDEAPELFLFQGELIDAARNEVKYQARGDQRIILYDISY
                     R"
BASE COUNT          660 a         1335 c         1501 g          601 t
ORIGIN      
        1 gacgatctcg aggcccatcg tcttcttgcc ctcgaagcga tagggctcgc gtccggcccc
       61 cgcgaggagc cagccgtggc gagcggacgc ctcgcccatc atccggaaca cgtcgccgac
      121 ggtgccgtcg acgcggtaga cgtgcgcgcc gttggtcaag ctgcccttcc agccgagatc
      181 ggggcaggag accgggagcg cggtatggat ctcgacgccc gcgcgcgccc cgtaggcggc
      241 ccacgcgtgc ccgccgctgc ccacggtggg ctgggcgaag cgggtgacac cgagctcctt
      301 ccagcgcgag atcgacgcgg aggcgccgcg cgccttgaag gtcccggtgg ggttgacgcc
      361 ctcgtccttc acccagagct cgccgacccc aacctcgcgc gccgccttcg gcaccgggat
      421 catcggggtg aagccctcgc ccatggtcac gatcgacgcc tcgtcgcgga ccgggagcag
      481 ctcgcggtag cgccacatgg tgggcgggcg ccgcgcgatg tcatccggag tgaccgcctt
      541 gcgcacgacg gccaggtcgt agcggaccag cagcgtgctc ccgcagtcgc agagcgcccg
      601 gggggtgtcg ccgggatagt agtcggagca gcgcgtgcat tcgaggccgg ccagataacc
      661 agtcattgga tgatttctcc ctcgtggggc gcggactcag ccgcggcggg cccggccgcg
      721 gaggcgcggc tcgagcaggt cgcgggcgcc gtcgcccagc acgttcatgg atagcacgat
      781 catcatgatg gcgagcccgg gaaacgtggc gagccaccag ccgttgatca ggtactcctt
      841 gccgtcggcg agcgccatgc cccagctcgg ggtcggcggg ggcaccccga gcccgaggaa
      901 gctgagggtg gactcgagga cgatcaggcg cccgatctcg gtggtcatga tcacgagcgc
      961 gtaggagagc acgttgggca ggacgtggcg cagcaggatc caccaggcgg ggcagccgag
     1021 gccgcgggcg gcctggatga agtcggtctc gcgcaggctc aaggtctgcc cgcgcaccac
     1081 gcgcgcgtag gtcacccagc cgctcagcgc caggatgatc agcagattgg gcaggctcgg
     1141 gcccagcacc gcgatgaccg tgatcgcgaa gagcaggaac gggaaggcga gctgcacgtc
     1201 gatgagcttg gccaccaccg agtccgcccg gccgcggaag tagccgccga gcatgccgag
     1261 ggtgagcccg atggtgccgc cgatgaggga cgagaccacg ccgaccagga gcgagacgcg
     1321 ggcgccgaag atgatccggc tcaggatgtc gcgcccgagc tggtcggtgc cgagcaggta
     1381 ccgggcgctg cccgccgcgg tccacgcggg cggccgcagg cgcgcggaga gatcgccgcg
     1441 ggtcgggtcg tagggggcga gctgcggggc cagcaccgcg cagatcaccg ccgcgacgac
     1501 cggggcggcg gcgaggagca ggagcggggg cacccgaggg cgcatcaggc ctggagcttg
     1561 acgcggggat cgagccacgc gtagcacagg tcgaccagga gattggcgag gatgaacacc
     1621 atcgccatca cgaacacggc cgcttcgacg aggggaaaat cgcggttgta gatcgcgaag
     1681 atcatcagcc ggcccacgcc cggccacgcg aagatcatct cgcagacgat cgcgccgccc
     1741 agcagcaggc cgaactggag cccgatcacg gtgacgatcg gcatcgccgc gttcttgagc
     1801 gtgtgcttga gcaccacgcc gcgctcgggc agccccttgg cccgcgcggt gcggacgtag
     1861 tcggcgtcga gcacctcgag catgccggat cgcaccaggc gcgccatcgc ggccatcgga
     1921 tagctggcga gcgtcagggc cggcaggatc aggtgttgcc aggtgccgta gccggaggtg
     1981 ggcagcagcc ggaggatgac cgagcccacc agcatcagca tcaggccggt ccagaagatc
     2041 ggggcggact gccccagcgc ggcgaggccg atggccacgc ggtcgagcgc ggagccgcga
     2101 tgcgcggccg cgaggatgcc gagcggcagc gcgaccgcca gcgcgagcag cagcgcgctg
     2161 ccggccagga gcatcgaggc gggcagccgc tcggccacca ggcccatcgc gggctgctga
     2221 tgctggagcg acacgccgaa gtcgccgtgc gcgacccggg cgaggaagcg gccgtactgc
     2281 acgatgagcg gctgatcgag cccgaggctc ttcgtgagcg cgtcgatctc ctcgcggctc
     2341 gcgttggacg gcagcatcat catcaccggg tcgccggtga ggtggagcac cacgaacacc
     2401 agggtgagca cgcagaacag cacgatcagg gtgtgcccga gcttggcgag gaggaagtcg
     2461 cgcatgcgga gatcagctcg gcgcccggct ctgttcggcc gggcgccgac cgtggaccgt
     2521 cagatcaacg ataagaaata tcgtagagga tgatgcgctg atcgccccgc gcctggtact
     2581 tcacctcgtt gcgggccgcg tcgatcagct cgccctggaa gaggaagagc tcgggcgcct
     2641 cgtcgtgcca gatctgggcc gcctcctggt agttgcgcag ccgcttcggc acatccaggg
     2701 tgacgcgagc ctcctccatc agcttgtaga acggagtccc ttcgtagttg cccggccagg
     2761 cctggtggag gatgccgccc ggcccgcgga ggcgccagat caccccgtcg gtgtcgtagg
     2821 tggtgctcgc ggggtcggcc aggagcaggc ccgggaaggt gttggtgcgc aggagctggc
     2881 tccgctgcga gggctcgagc accccgacct tggggcgcac ccccacgtcc tggagcatca
     2941 ccgccaccgc ctcggagagc tggcggtcgt tgaggtggat gccgttgccg ctctggatgt
     3001 cgacgtcgac cccgttcggg tacccggcct cggccaggag ctgccgcgcc cgcgcggggt
     3061 cgtaggggta gggcttgatc ttggcgttgt agccgaagtc ggtgttcggg attccgctcc
     3121 cgatcgggat cgcgtagccc ttgtagagct ccttgatgat cttgtccttg tcgaccgcgt
     3181 agttgagcgc ctggcgcacc ttcgtattcg ccagcgggcc cttctgggcg ttgatcaggt
     3241 acaccgtcac caggttggtc agggtgtgct cgacgcggca gcagcccgcc ttctccacct
     3301 gcgacacgtg gtccgggatg accttggtga tgatctgggc ctcgccgcgc gtcagagccg
     3361 ccacccgggc aaagttgtcg gggatcggcc gccagacgac cttcttgatc ttgggggcgc
     3421 cgcgccagta ggcggggttg gcctcgagca ccacccggtc gtccttcacc cactccacga
     3481 acttgtacgg cccggtgccc accggcttga tcgcgaaggt cttccagtcc acctgcttga
     3541 tgtactgcgg gggcaggatc tgcccgccgt agcccgcgaa gcgcttgacc aggatcggat
     3601 cgggcttgct ggtcacgatg ttgatcgtgt acgcgtcgac cacgtcggcg cgctcgatgg
     3661 tgctgaagcc gccgtagacc tggtgcacgc ccttggtgat caggcgatcg acggtgaact
     3721 tcaccgcgtc cgcgttgaac gactccccgt tgtggaagct gaccccctgg cggagcttga
     3781 actgccaggt gagcggatcg acgctgcgcc aggagacggc cagctcgggc acgagcttgc
     3841 cgatgtcctt cgggtcccac gtcaccagcg cgtcgtagat gttgtagaag aggttcaggt
     3901 tgtggatctg ggtcgcctgg gtcggatcga gcgtggtgat gtcggcgccc tgggcgatca
     3961 ccaggtcctg gccgacggcc ggagccgcgg ggagcgcgat cgcgagcagg gctaggcaca
     4021 gaccgagcgt gacggcgaga acggtgcgtt gcatcaaggc tcctccgtgg agtgggaaac
     4081 ggcgatgacg atcagaa
//
LOCUS       DATMCV010000485         2152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_161481, whole genome shotgun sequence.
ACCESSION   DATMCV010000485 DATMCV010000000
VERSION     DATMCV010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161481"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VNU03_11200"
     CDS             <1..228
                     /locus_tag="VNU03_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWP75077.1"
                     /translation="HFPQHAWDWFPWAGFSYPFNFTGQPAATVPAGFTASGMPVGLQI
                     VGRRLADLQVLQASAAFEEARPWAAKRPALG"
     gene            245..583
                     /locus_tag="VNU03_11205"
     CDS             245..583
                     /locus_tag="VNU03_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HWP75078.1"
                     /translation="MWIMVSGPYRSGAADAEQRTRNLAVLNEAALALFRAGHVPIIGV
                     NVALPIIESAGASAFDEIMMPLSLALVDRCDACLRVGGASTGADQEAQRFRATGRPVY
                     GSVAEVPGTR"
     gene            580..1248
                     /locus_tag="VNU03_11210"
     CDS             580..1248
                     /locus_tag="VNU03_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP75079.1"
                     /translation="MSETRPYDVITFDCYGTLIDWDGGIAGAFAHAIAESGGGLDPHR
                     VMEVYEEIEAEVEAERYRSYREVLTESTRRIARRLGWALPEAKAGFLAESLPRWVPFP
                     DTNAALERLFKAGYRLGILSNVDDDLLAGTRKHFTVPFDPIVTAQQVGSYKPADGHFV
                     TARGRIGTARWLHAAQSHFHDVTPARRHRIASAWINRKHLVRGEPRPDRELHSLTELA
                     DWLA"
     gene            1258..1716
                     /locus_tag="VNU03_11215"
     CDS             1258..1716
                     /locus_tag="VNU03_11215"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWP75080.1"
                     /translation="MALEQALTDTLTQAIKAKDGKTSDVVRMIKTKIQERRTAKGASG
                     QVDDALVLEVIGAYRKQLQKALAEFDKVGERGAAQAAVLRFEIEFCERYLPKTMDEAG
                     VRALVKERIAALGIADPKQAGRLVGDIMKTHKGQVEAGDVKRVADELLKG"
     gene            complement(1726..>2152)
                     /locus_tag="VNU03_11220"
     CDS             complement(1726..>2152)
                     /locus_tag="VNU03_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWP75081.1"
                     /translation="LLLTPGVAAVILNDAGHVLLQRRSDDGRWGLPGGAMEPGEEPAE
                     SLVREVREETALEVVPERIVGVYSGPDFRVRYGNGDEAMIVSITFACRSLGGEPRIND
                     DESLELRYFPPDALPAMESRHRMRIEDALRDDPRARFRR"
BASE COUNT          315 a          782 c          768 g          287 t
ORIGIN      
        1 cacttcccgc agcacgcgtg ggactggttt ccgtgggcgg gcttcagcta cccgttcaac
       61 ttcaccggcc agcccgccgc gacggtgccc gcgggcttca ccgcctccgg catgccggta
      121 gggctgcaga tcgtcggccg gcgcctcgcc gatctccagg tgctgcaggc ctccgcggcg
      181 ttcgaagagg cgcggccgtg ggcggcgaag cgcccggcgc tcggctgagc gacggcccgg
      241 ccacatgtgg atcatggtgt cggggcccta tcgcagcggc gcggccgatg ccgagcagcg
      301 cacgcggaac ctggccgtgc tgaacgaggc cgcgctcgct ctctttcgtg ccgggcacgt
      361 gccgatcatc ggcgtcaacg tggcgcttcc gatcatcgag tccgccggcg cgtcggcctt
      421 cgatgagatc atgatgcccc tctcgctcgc cctggtggac cgctgcgacg cgtgcctgcg
      481 cgtgggcggc gcatccaccg gagcggacca ggaggcccag cggttccgcg cgacgggccg
      541 cccggtctac ggcagcgtcg cggaggtgcc ggggacccga tgagcgagac gcgcccgtac
      601 gacgtcatca cgttcgactg ctacggcacc ctgatcgatt gggacggggg catcgcgggg
      661 gcgttcgccc acgcgatcgc ggagagcggc ggcgggctcg acccgcaccg cgttatggag
      721 gtctacgagg agatcgaggc ggaggtggag gccgagcgct accggagcta ccgcgaggtg
      781 ctcaccgaga gcacgcggcg catcgcccgg aggctcggct gggcgctgcc ggaggcgaag
      841 gccggcttcc tggccgagag cctgccgcgc tgggttccgt tcccggacac caacgccgcg
      901 ctcgagcggc tcttcaaggc cggctaccga ctcggcatcc tgtccaacgt ggacgacgat
      961 ctcctcgcgg gcacccgcaa gcacttcacg gtgccgttcg acccgatcgt caccgcgcag
     1021 caggtcggct cctacaagcc ggccgacggc cacttcgtca ccgcgcgcgg gcgcatcggg
     1081 accgcgcgct ggctgcacgc cgcgcagagc cacttccatg acgtgacccc ggcccggcgc
     1141 caccgcatcg cgtcggcctg gatcaaccgc aagcacctcg tccgcggcga gccgcggccg
     1201 gatcgcgagc tccactcgct caccgagctg gccgactggc tcgcctaggg gagaccgatg
     1261 gcactcgagc aggccctcac cgacacgctc acccaggcca tcaaggccaa ggacggcaag
     1321 acgtccgacg tggtgcgcat gatcaagacc aagatccagg agcgccgcac cgccaagggc
     1381 gcctccggcc aggtcgacga cgccctcgtg ctcgaggtga tcggcgccta ccgcaagcag
     1441 ctgcagaagg cgctcgccga gttcgacaag gtgggcgagc gcggggcggc ccaggccgcg
     1501 gtgctccgct tcgagatcga gttctgcgag cgctacctgc cgaagaccat ggacgaggcg
     1561 ggggtccggg cgctggtgaa ggagcgcatc gcggccctcg gcatcgccga tcccaagcag
     1621 gccggccgcc tggtcggcga catcatgaag actcacaagg gccaggtgga ggcgggcgac
     1681 gtcaagcgcg tcgccgacga gctgctgaag ggctgagcgc tccgctcagc ggcggaagcg
     1741 ggcgcgcgga tcgtcgcgga gggcgtcctc gatccgcatc cggtgccgcg actccatcgc
     1801 gggcagagcg tcgggcggga agtagcgcag ctcgagcgac tcgtcgtcgt tgatccgggg
     1861 ctcgccgccc agcgagcggc acgcgaaggt gatgctgacg atcatcgcct cgtcgccgtt
     1921 tccgtagcgg acgcggaagt ccgggccgct gtagacgccc acgatgcgct ccggcacgac
     1981 ctccagcgcg gtctcctccc gcacttcgcg caccaggctc tccgcgggct cctcgcccgg
     2041 ctccatcgcg ccgcccggca gcccccaccg gccgtcgtcg ctccggcgct ggagcagcac
     2101 gtggcccgcg tcgttcagga tgaccgcggc gacccccggg gtcagcagga gg
//
LOCUS       DATMCV010000486         4770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_625702, whole genome shotgun sequence.
ACCESSION   DATMCV010000486 DATMCV010000000
VERSION     DATMCV010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625702"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="VNU03_11225"
     CDS             complement(<1..1438)
                     /locus_tag="VNU03_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HWP75082.1"
                     /translation="MIDGRPTNPFAPFDRADLEGAIGARFERQVDRAPARLAVKMGAE
                     AQSYRALDSAANRVAHVLLDRLGPDNEPVVLLLPQSPGQVAGVLGALKAGKIYVPLDP
                     THPPARLSEAIADSDARLVLTAAAHVDLARRVAPGRSVVAIEDGAAAPDRRPGLTVSP
                     EAGAYIFYTSGSTGRPKGVLDTHRNVLHNVMRYTNTLHLGPDDRLTLLQGPAFSGAVS
                     SLFGALLNGAASFPFDVPREGANRIAPWLREEAITIYHSVPALFREVVTHGATLPALR
                     IVRLEGDQASVRDLEAFQRHFAADCVLVNGLGATECGLVRQFFFTPGHALPDGVAPIG
                     EPVEDMEIVLLGETGAPVLEGATGEIAVRSRYLAAGYWRRAELTAERFLDDRERPGVR
                     LYRTGDLGRFQAGGILEHLGRVDGLAKVRGQRVEAADVESALLALPGVAAAAVTVRED
                     GPGEARLIAYLVPGAAPLPSPTEMRRALA"
     gene            complement(1435..1866)
                     /gene="arfB"
                     /locus_tag="VNU03_11230"
     CDS             complement(1435..1866)
                     /gene="arfB"
                     /locus_tag="VNU03_11230"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWP75083.1"
                     /translation="MADPIVVTGEVRVPASALTVHAVRASGPGGQNVNKVATKIDLRV
                     DLEAIEGLADAARARLRVLAQSRVDAEGRLVVTSQATRNQARNLEDARARVAELVRAA
                     LVAPRPRVKTRPSAGARRRRLAGKKQRADVKRWRSRPDEGE"
     gene            complement(1845..2369)
                     /locus_tag="VNU03_11235"
     CDS             complement(1845..2369)
                     /locus_tag="VNU03_11235"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWP75084.1"
                     /translation="MHFPASLGRKQRLLAGVLGIGVGFGGPFLGSVGLVAATGDPTAL
                     ILPLPFLGALWAIQGLAPSGYTLEEHGVRLERRWLRRLIPYDRITGCDRRRRPIGGLL
                     AAGLNGLFGSHGPRWNPRSGWHYLAITNTADLVFLETPGGFVVLSPSRPDEFVAALSA
                     RLPTGSAPWPTRSS"
     gene            complement(2373..2798)
                     /gene="ndk"
                     /locus_tag="VNU03_11240"
     CDS             complement(2373..2798)
                     /gene="ndk"
                     /locus_tag="VNU03_11240"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWP75085.1"
                     /translation="MAVQRTLTIIKPDAVAKGVAGQIIARFEKAGLKLLAAKLIHLSA
                     TQAGGFYIVHKERPFYASLCAFMTQGPCMPIVLEGENAIARLRELMGATDPAKADAGT
                     IRKDFASSIEANAVHGSDSPESAAFEIPYFFGALEIFAR"
     gene            complement(2812..3690)
                     /gene="sucD"
                     /locus_tag="VNU03_11245"
     CDS             complement(2812..3690)
                     /gene="sucD"
                     /locus_tag="VNU03_11245"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWP75086.1"
                     /translation="MSILVDKKTRVLVQGITGKEGAFHAARCKEYGTQVVGGVTPGKG
                     GTTHEGFAVWNTVEEATRAAGADCALIFVPPPAAADAIMEAADAGIPLVVCITEGVPV
                     ADMVRVKAFLARTSSRLIGANCPGLITPGQAKIGIMPGHIHKVGNVGVVSRSGTLTYE
                     VVHQLTQRGLGQSSCVGIGGDPVNGTNFIDVLTLFQADPDTTSIMMIGEIGGTAEEEA
                     AAFIKAHVTKPVVGFICGQTAPPGRRMGHAGAIIAGGKGTAAEKMAAMEAAGITTVKS
                     PADMGATVARVVGKKG"
     gene            complement(3687..4757)
                     /gene="sucC"
                     /locus_tag="VNU03_11250"
     CDS             complement(3687..4757)
                     /gene="sucC"
                     /locus_tag="VNU03_11250"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWP75087.1"
                     /translation="MTQKLGGKVVVKAQVHAGGRGKAGGVKLADDAAGAEAAAQQILG
                     MMLKTPQTPPEGIKVLKVLVEEASPIATELYLSMTLDRGRGTHVVMASQAGGMEIEEV
                     AATHPEKIIREWTDPALGLQAFQARNLAFGLGLTGEQFKQGVALILNLFRAYLEKDCS
                     LAEINPLVVTKDGRVMALDAKLNFDDNALYRHKEIVALRDVNEETPLDVEASKYSLNY
                     IKLDGNVGCMVNGAGLAMATMDIVKLAGGEPANFLDVGGGASPEQIENAFRILSSDPS
                     VKAVFINVFGGILRVDRLAEGIISAVKKLGLKLPVVLRAEGTNVEQGKKMLAESGLSL
                     VMADDMGEGAKKVVALARGAQA"
BASE COUNT          635 a         1721 c         1725 g          689 t
ORIGIN      
        1 cggcgagcgc ccggcgcatc tcggtcggcg acggcagcgg agccgcgccc ggcaccaggt
       61 aggcgatcag ccgcgcctcg ccgggcccgt cctcgcgcac cgtcaccgcg gccgccgcga
      121 cgccgggcag cgccagcagc gcgctctcca cgtccgcggc ctccacgcgc tggccgcgga
      181 ccttggcgag cccgtccacg cggccgaggt gctccaggat cccgcccgcc tggaagcgcc
      241 cgaggtcgcc ggtgcggtag agccgcacgc cgggccgctc gcggtcgtcg agaaagcgct
      301 ccgcggtcag ctcggcccgc cgccagtagc cggccgcgag gtagcggctg cgcaccgcga
      361 tctccccggt ggcgccctcg agcaccggcg ccccggtctc gcccaggagc acgatctcca
      421 tgtcctcgac cggctcgccg atcggggcca cgccatcggg cagcgcgtgg ccgggcgtga
      481 agaagaactg gcgcacgagc ccgcactcgg tggccccgag cccgttgacc aggacgcagt
      541 ccgccgcgaa gtgccgctgg aaggcctcca ggtcacgcac cgaggcctgg tcgccctcca
      601 ggcggacgat ccgcagcgcg ggaagcgtgg cgccgtgggt caccacctcg cggaagaggg
      661 ccggcaccga gtggtagatc gtgatcgcct cctcgcgaag ccagggcgcg atccggttcg
      721 cgccctcgcg cggcacgtcg aagggaaacg aggccgcgcc gttgagcagg gcgccgaaga
      781 ggctcgagac cgcgccgctg aaggcggggc cctggagcaa agtgagccgg tcgtcggggc
      841 cgaggtggag cgtgttggtg tagcgcatca cgttgtggag cacgttgcgg tgggtatcga
      901 gcacgccctt gggccggccg gtggagccgg aggtgtagaa gatgtacgcg cccgcctcgg
      961 gcgacacggt cagcccgggc cgccggtcgg gcgccgcggc accgtcctcg atcgcgacca
     1021 ccgaccgacc cggcgcgacc cgccgggcga ggtcgacgtg cgcggcggcg gtcagcacca
     1081 ggcgcgcgtc cgagtccgcg atcgcctcgc tgagacgggc cggcgggtgc gtcgggtcga
     1141 gcggcacgta gatcttgccg gccttgaggg ctcccagcac gcccgcgacc tgccccgggc
     1201 tctgcgggag cagcagcacc accggctcgt tgtcgggtcc gagccggtcc agcaggacgt
     1261 gggccacgcg gttcgccgcg gagtcgagcg cgcgatagct ctgcgcctcc gctcccatct
     1321 tgaccgcgag gcgggcgggc gcgcggtcga cctggcgctc gaagcgggcg ccgatcgcgc
     1381 cctcgaggtc ggcgcgatcg aacggcgcga acgggttggt gggccgcccg tcgatcactc
     1441 gccctcgtcc gggcggctgc gccagcgctt cacgtcggcc cgctgcttct tgcccgccag
     1501 ccggcgacgc cgggccccgg ccgacggcct ggtcttcacg cgcgggcggg gcgcgacgag
     1561 cgcggcgcgg accagctctg ccacgcgggc gcgggcgtct tccaggttcc gcgcctggtt
     1621 gcgggtcgcc tggctcgtca cgaccagccg cccctccgcg tcgacgcgcg actgcgccag
     1681 gacgcgcagg cgggcccggg ccgcgtcggc cagaccctcg atcgcctcca ggtccacgcg
     1741 caggtcgatc ttggtcgcga ccttgttcac gttctgcccg ccgggcccgg aggcgcgcac
     1801 cgcgtggacc gtgagcgcgg aggcgggcac ccgcacctcc ccggtcacga cgatcgggtc
     1861 ggccacggcg cggatccggt cggcaggcgg gcgctcagcg cggcgacgaa ctcgtcgggg
     1921 cggctcggcg agagcaccac gaagccgccc ggcgtctcca ggaacaccag gtcggcggtg
     1981 ttggtgatgg ccaggtagtg ccagccgctg cgcgggttcc agcgggggcc gtgggagccg
     2041 aagagcccgt tgagcccggc ggcgagcagc ccgccgatcg gccggcggcg gcgatcgcac
     2101 ccggtgatgc ggtcgtaggg gatgaggcgc cggagccagc gccgctcgag ccgcacgccg
     2161 tgctcctcga gggtgtagcc gctcggggcg aggccctgga tggcccagag cgcgccgaga
     2221 aacggcaggg gcaggatcag cgcggtcgga tcgccggtgg cggccaccag ccccaccgag
     2281 ccgaggaagg gcccgccgaa gccgaccccg atacccagca ccccggccag caggcgctgc
     2341 ttccggccga gggacgccgg gaagtgcact ccctacctgg cgaagatctc gagcgcgccg
     2401 aagaagtagg ggatctcgaa ggcggccgac tcgggcgagt cggagccgtg gaccgcgttg
     2461 gcctcgatcg acgacgcgaa gtccttccgg atcgtccccg cgtcggcctt ggccggatcg
     2521 gtggcgccca tgagctcccg caggcgcgcg atcgcgttct ccccttccag cacgatcggc
     2581 atgcacggtc cctgggtcat gaaggcgcag aggctcgcgt agaacggccg ctccttgtgc
     2641 acgatgtaga agccaccggc ctgcgtcgcg ctcaggtgga tgagcttggc cgcgaggagc
     2701 ttgagcccgg ccttctcgaa gcgggcgatg atctggcccg cgacgccctt ggcgacggcg
     2761 tccggtttga tgatggtgag agtgcgctgg acggccatcg cggacgggtc cctacccttt
     2821 cttgcccacc acgcgggcga cggtcgcgcc catgtcggcg gggctcttga cggtggtgat
     2881 gccggcggcc tccatcgcgg ccatcttctc tgccgccgtc cctttacctc cggcgatgat
     2941 ggcgccggcg tggcccatgc gacggcccgg cggcgcggtc tggccgcaga tgaagccgac
     3001 gaccggcttg gtgacgtggg ccttgatgaa ggcggccgcc tcttcctccg cggtgccgcc
     3061 gatctccccg atcatcatga tcgaggtggt atcgggatcg gcctggaaga gggtgagcac
     3121 gtcgatgaag ttcgtcccgt tgaccgggtc gcccccgatg cccacgcagg acgactggcc
     3181 gagcccgcgc tgggtgagct ggtggaccac ctcgtaggtg agggtgccgc tgcgcgagac
     3241 caccccgacg tttcccacct tgtggatgtg gcccggcatg atcccgatct tggcctggcc
     3301 cggcgtgatc aggcccgggc agttggcgcc gatcaggcgg ctcgaggtcc gcgcgaggaa
     3361 ggccttgacc cgcaccatgt ccgccaccgg caccccctcg gtgatgcaga ccaccagagg
     3421 aatccccgca tccgcggctt ccatgatggc gtctgcggcg gccggaggcg gcacgaagat
     3481 cagcgcgcag tcggcgccgg cggccctggt cgcctcctcc accgtgttcc agaccgcgaa
     3541 gccctcgtgg gtggtgccgc ccttgccggg cgtgaccccg cccaccacct gggtgccgta
     3601 ctccttgcag cgcgcggcgt ggaaggcgcc ctccttgccg gtgatgccct gcacgaggac
     3661 gcgggtcttc ttgtcgacga ggatgctcat gcctgggcac cccgggccag cgccacgacc
     3721 ttcttcgcgc cctcgcccat gtcgtcggcc atcaccagcg aaaggccgga ctcggccagc
     3781 atcttcttgc cctgctcgac gttggtgccc tcggcccgca gcaccaccgg cagcttgagc
     3841 ccgagcttct tgaccgcgga gatgatgccc tccgcgagcc gatccactct caatattcct
     3901 ccgaagacgt tgatgaaaac cgccttcacg ctcggatcgg acgagaggat gcggaacgcg
     3961 ttctcgatct gctcggggga ggccccgccg cccacgtcga ggaagttggc gggctcgccg
     4021 ccggccagct tgacgatgtc catggtggcc atcgcgagcc cggcgccgtt caccatgcag
     4081 ccgacgttgc cgtcgagctt gatgtagttg aggctgtact tcgaggcctc gacgtcgagc
     4141 ggggtctcct cgttgacgtc gcgcagggcc acgatctcct tgtggcggta gagcgcgttg
     4201 tcgtcgaagt tgagcttcgc gtcgagcgcc atcacgcgcc cgtccttggt gaccaccagg
     4261 ggattgatct cggctagcga gcagtccttc tcgaggtagg cccggaagag attgaggatc
     4321 agggccacgc cctgcttgaa ctgctcgccg gtgagcccga gcccgaaggc caggttgcgc
     4381 gcctggaagg cctggagccc gagcgcgggg tcggtccact cgcggatgat cttctccggg
     4441 tgggtggccg ccacctcctc gatctccatg ccgcccgcct gcgaggccat caccacgtgg
     4501 gtaccgcgcc cgcggtcgag ggtcatcgag aggtagagct cggtggcgat cggggaggcc
     4561 tcctccacca ggaccttcag caccttgatg ccctcgggcg gggtctgcgg ggtcttgagc
     4621 atcatgccga ggatctgttg ggccgccgcc tccgcgcccg ccgcgtcgtc ggccagcttc
     4681 acgccgcccg ccttgccgcg cccgcccgcg tgcacctggg ccttcaccac caccttgccg
     4741 cccagcttct gggtgatgac gcgcgcctgg
//
LOCUS       DATMCV010000487         2473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_492822, whole genome shotgun sequence.
ACCESSION   DATMCV010000487 DATMCV010000000
VERSION     DATMCV010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492822"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..434)
                     /locus_tag="VNU03_11255"
     CDS             complement(66..434)
                     /locus_tag="VNU03_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HWP75088.1"
                     /translation="MSDVRIDKWLWAARFFKSRTLAATACAGGKVDVNGDAAKPSKTV
                     RPGDLLQITLPRSRRIVRVAVLAERRGSGTEAALLYEDLTPPPPPPREARAAPPAVRP
                     PGAGRPTKRERRLIDRLQRY"
     gene            482..1171
                     /locus_tag="VNU03_11260"
     CDS             482..1171
                     /locus_tag="VNU03_11260"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HWP75089.1"
                     /translation="MIRWLVLPTTTALLAVSLIAGPLRAADPPTVPVEDWAKQTDGKT
                     GIPDGWKGQNWGSPKYDFRIATQGGRKAINLKSANDSSTISKEVKVDVKTWPVLQWSW
                     QVVTLPKGGDARKSATDDEAAQIYVTFPRFPQAVRSRIISYIWDTAAPVGSVFKSEKT
                     GLVTYVVVRSGPADLGKWLTESRNVLEDYKKIYGEAPGEDVGAISIAIDSNDTNSTAE
                     SYFGEIFFRKP"
     gene            1190..1573
                     /locus_tag="VNU03_11265"
     CDS             1190..1573
                     /locus_tag="VNU03_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWP75090.1"
                     /translation="MAAYGATLLRLILGIVYIMHAYLAAVVFGPAGMIAYQTKNGVPF
                     AEIGTWYLILAHGLGGICLVLGLFTRWAALANVPVMLGAVLFVHLKNGFWAHSNPNGY
                     EYALVLLVVSLAVAMIGGGALALRR"
     gene            complement(1590..2240)
                     /locus_tag="VNU03_11270"
     CDS             complement(1590..2240)
                     /locus_tag="VNU03_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP75091.1"
                     /translation="MKAEGDAFLAKGDYGNAVEKYRQAIDLDSGAIGPRFALGTAYSF
                     MEKRPEAIVQFRWVLSRADAASPEYQEAQRWLIRVGALAPPTAVAVADQKGEARPVDP
                     STVGRLVGKTEWPGVTPQRRMLSGTMSLIGDEPVTQDVKRNRAFRLGDGYEFKDVPAG
                     RYRVVAVIDGTTIWDEKVTVETGKDTSLTLSQSTSPVPAGKLADKPDAPATAERSR"
BASE COUNT          376 a          828 c          872 g          397 t
ORIGIN      
        1 ccggatgcga cgggtcaacc gcccgccggc gcgtcaccgt gcggctccac cggaggccgg
       61 cgcgatcagt agcgctgcag gcggtcgatc aggcggcgct cccgcttcgt cggacggccg
      121 gccccgggcg gccgcaccgc aggcggcgcg gcgcgcgcct cgcgcggcgg agggggcggc
      181 ggggtgaggt cctcgtacag cagcgcagcc tcagtgccgc tgccgcgccg ctccgccagg
      241 accgccaccc ggacgatccg ccggcttcgc gggagcgtga tctggaggag atccccgggg
      301 cgcaccgtct tggacggctt cgcggcgtcg ccgttgacgt ccaccttccc tcccgcgcac
      361 gcggtggcgg cgagggtgcg gctcttgaag aagcgggccg cccacagcca cttgtcgatc
      421 cgcacgtccg acatcaggtt tttaccgtag cacagggggg gcgtgagaaa ctgaggcggt
      481 catgatccgc tggctcgttc tcccgacgac gaccgcgctc ctggcggtct ccctgatcgc
      541 cggtcctctc cgcgccgccg accctccgac cgtgccggtc gaggactggg ccaagcagac
      601 ggacggcaag accgggatcc cggatgggtg gaagggccag aactggggaa gtcccaagta
      661 cgactttcgc atcgccacgc agggtggccg caaggcgatt aatttgaaga gcgcgaacga
      721 cagctcgacg atctccaagg aggtgaaggt cgacgtcaag acctggccgg tcctccagtg
      781 gagctggcag gtggtgaccc tgccgaaggg gggcgacgcc cgcaagtcgg cgaccgacga
      841 cgaggcggcc cagatctacg tcacgttccc gcgctttccg caggcggtgc gctcgcggat
      901 catctcctac atctgggaca ccgccgcgcc ggtcggatcc gtcttcaaga gcgagaagac
      961 cgggctcgtg acctacgtcg tcgtgcgctc cggccccgcc gacctcggca agtggctcac
     1021 cgagagccgc aacgtactgg aggactacaa gaagatctac ggggaagcgc cgggcgagga
     1081 cgtgggggcg atctcgatcg ccatcgactc caacgacacg aactcgaccg ccgagtccta
     1141 cttcggggag atcttcttcc gcaagccgta gcccacgagg agaggtgcaa tggccgcgta
     1201 cggcgcaacg ctgctccggc tcatcctggg gatcgtctac atcatgcacg cctacctcgc
     1261 ggccgtggtc ttcggtccgg ccggcatgat cgcctatcag accaagaacg gcgtgccgtt
     1321 cgccgagatc gggacctggt acctgatcct ggcccacggg ctggggggga tctgcctcgt
     1381 gctgggcctg ttcacgcggt gggccgccct ggccaatgtg ccggtgatgc taggggcggt
     1441 gttgttcgtt cacctcaaga acgggttctg ggcgcattcg aacccgaatg gctacgagta
     1501 cgcgttggtc ttgctggtgg tctccctcgc ggtggcgatg atcgggggag gggcgctcgc
     1561 cctcaggcgg tagcgcgccg agcgggcggt cagcgcgagc gttcggcggt agccggggcg
     1621 tcgggcttgt cggcgagctt cccggccggc accgggctcg tcgactggct cagcgtgagg
     1681 ctcgtatcct tgccggtctc cacggtgacc ttctcgtccc agatcgtcgt tccgtcgatc
     1741 accgcgacca cccggtagcg acccgcgggg acgtccttga actcgtaacc gtcgcccagt
     1801 cggaacgcgc gattgcgctt gacgtcctgg gtgaccggct cgtcaccgat cagggacatg
     1861 gtgccactga gcatgcggcg ctgaggagtc acccccggcc attccgtctt tccgacgagt
     1921 cggccgaccg tcgacgggtc taccgggcga gcctcgccct tctggtcggc gaccgccacc
     1981 gcggtcggcg gcgccagcgc gcccacacga atcagccacc gctgtgcctc ctggtactcg
     2041 ggggaggcag cgtcggcgcg gctcaggacc cagcggaact gcacgatcgc ctcggggcgt
     2101 ttctccatga aggaataggc ggttcccagc gcgaaccgcg ggccgatcgc gccagagtcg
     2161 aggtcgatcg cctggcggta cttctcgacc gcattgccgt agtcaccctt ggcgaggaag
     2221 gcgtcgccct cggccttcat ctgctcgacc ggggacagat tctgcgaggt ggggggatgg
     2281 gagaccggcg cctgctggca ggcggtcccc aggaggagcg ccgccgccag caggatgatg
     2341 cgtcgcacgc ctagttctcg cggaagccgg agatgcgggt gttgtagagg ccgcggaggg
     2401 ccaggtcaat gccctccttg ccgaagcgga tcttcagggt atcggcctgg ttcaggaact
     2461 cgaagtagcc gcc
//
LOCUS       DATMCV010000488         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_23377, whole genome shotgun sequence.
ACCESSION   DATMCV010000488 DATMCV010000000
VERSION     DATMCV010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23377"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VNU03_11275"
     CDS             complement(<1..554)
                     /locus_tag="VNU03_11275"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWP75092.1"
                     /translation="MLSFIFNEAMRDLRRAGRVGVSAILLIALSLTALGGFWLLSLNL
                     GRAIDQWGDRVRVIVYLKDEPPAARVEELMQRIEAVGGIQRLRYVSKAEALRVLKRSL
                     GSQADVADQLPRNPLPASVEVTPDAAIATPEGTRALVGRLVLLPEVEEVQGGSQWVDG
                     LAQFRRLFQGMGLVVGAVLALAAI"
     gene            complement(554..1225)
                     /locus_tag="VNU03_11280"
     CDS             complement(554..1225)
                     /locus_tag="VNU03_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP75093.1"
                     /translation="MIRLQHVSKVFGAGPSRVSALNGISFSVAKGEFVVLTGASGAGK
                     TTLLRMLYRAETPSEGDIEVAGFDVTELRRSEIPRLRRSIGIVFQDSKLLAGRTVFEN
                     VAFVLRVLGTPRRDITPKAFAALKAVGLSARAQAFPNQLSQGEAQRASLARAIVKSPG
                     LLLADEPTGNLDEDMAGEILGLVKEIWSRGTTVLFATHQARLVPLLKRRTLRLEGGRL
                     VKDEG"
     gene            complement(1253..2710)
                     /gene="gatB"
                     /locus_tag="VNU03_11285"
     CDS             complement(1253..2710)
                     /gene="gatB"
                     /locus_tag="VNU03_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWP75094.1"
                     /translation="MTGAARYETIVGLEVHAQLSTVTKMFCGCRAVFGAPPNTATCPV
                     CQGMPGSLPFINRRAVEFGIMTALAIGCGINAHNRFARKHYYYPDMPKNYQISQYEEP
                     LAEHGRLVIDTGPGPREIGIQRVHLEEDVGKLLHEGALETAQSSSVDYNRAGVPLMEI
                     VSKPDLRSPDEAAAYLRAVRAIVVYLGVCDGNMEEGSLRCDANISLRPRGQTEYGTKV
                     EIKNLNSFRNVRDALEHEIKRQTRALDTNERIVQETRLWDPDRMVTVSMRSKEFAHDY
                     RYFPEPDLPPLDVQPAWVEAVRARLPELPEARRARFQADYALSAYEADLLTQGRGLAD
                     YFEAAARAGGKPKSVANWVLNELLRELPGDDDRAIGACPIPPANLAGLLALIDDGTIS
                     GKIAKDVFEKMYRSGDTAQAIVSREGLTQVADEGALGAAVDRVLGEHAKVVEDFRAGK
                     KAALGFLVGQVMKATQGKANPGLVNRLLIEKLPKV"
BASE COUNT          415 a          986 c          994 g          440 t
ORIGIN      
        1 aagatcgcgg ccagcgccag caccgcgccc accacgaggc ccatgccctg gaagagccgg
       61 cggaactggg cgagaccgtc gacccactgg ctgccgccct ggacctcctc cacctcgggc
      121 agcaggacga gccgtcccac cagcgctcga gtgccctccg gcgtggcgat cgccgcgtcc
      181 ggcgtcacct cgacggacgc gggcagcggg ttacggggca gctggtcggc cacgtcggcc
      241 tggctgccga gcgagcgctt gagcacgcgc agcgcctccg ccttggacac gtagcgcagg
      301 cgctggatgc cgcccaccgc ctcgatccgc tgcatgagct cctcgacgcg cgcggcgggc
      361 ggctcgtcct tcaggtagac gatgacccgc acccgatcgc cccactgatc gatggcgcgc
      421 ccgaggttga gcgagaggag ccagaacccg cccagcgcgg tgagcgagag cgcgatgagc
      481 aggatcgcgc tgaccccaac gcgccccgcg cggcgcaggt cgcgcatcgc ctcgttgaaa
      541 ataaagctca gcactagccc tcgtccttca ccaggcgacc gccttctagt ctcagcgtgc
      601 ggcgcttcag cagggggacg aggcgggcct ggtgggtggc gaagagcacg gtggtgccgc
      661 ggctccagat ctccttgacc agcccgagga tctcgcccgc catgtcctcg tccaggttgc
      721 cggtcggctc gtcggccagc agcaggccgg gcgacttgac gatggcgcgg gccagcgagg
      781 cccgctgcgc ctcgccctgc gagagctggt tggggaaggc ctgcgcgcgg gcggagaggc
      841 ccaccgcctt cagggccgcg aaggccttgg gcgtgatgtc gcgccgcgga gtgcccagca
      901 cgcggagcac gaacgccacg ttctcgaaca cggtgcggcc ggccaggagc ttggagtcct
      961 ggaagacgat gccgatggag cggcgcaggc gcgggatctc cgaccggcgc agctcggtga
     1021 cgtcgaaccc ggccacctcg atgtcgccct cggagggggt ctcggcgcga tacagcatgc
     1081 gcagcagcgt ggtcttgccg gccccgctgg cgccggtgag caccacgaac tcgcccttgg
     1141 cgacgctgaa ggagataccg ttgagggcgg acacccggga agggcccgct ccgaagacct
     1201 tcgacacgtg ctgcagccga atcatgggct gcgttccatc ttacctaact attcagactt
     1261 tcggtagttt ctcgatcaga aggcggttca cgagcccggg attggccttg ccctgcgtgg
     1321 ccttcatcac ctgccccacc aggaagccga gcgcggcctt ctttcccgct cggaagtcct
     1381 ccacgacctt ggcgtgctcg cccagcaccc ggtccaccgc ggcccccagc gcgccctcat
     1441 cggccacctg ggtgagccct tcgcggctca cgatggcctg ggcggtgtcg cccgagcgat
     1501 acatcttctc gaacacgtcc ttcgcgatct tgccgctgat ggtgccgtcg tcgatcaggg
     1561 cgagcagccc ggccaggttg gcgggcggga tcgggcaggc cccgatcgcg cggtcgtcgt
     1621 cgcccggaag ctcgcggagc agctcgttga gcacccagtt ggccacgctc ttgggctttc
     1681 cccccgcgcg cgccgccgcc tcgaagtagt cggccaggcc gcggccctgg gtcaggagat
     1741 cggcctcgta ggccgagagg gcatagtcgg cctggaaccg cgcccggcgg gcttccggca
     1801 gctcgggcag gcgcgcccgc accgcttcca cccaggccgg ctgcacgtcg agcggcggca
     1861 ggtccggctc ggggaagtag cggtagtcgt gggcgaactc cttcgagcgc atcgagacgg
     1921 tcaccatgcg gtcgggatcc cagaggcgcg tctcctgcac gatccgctcg ttggtgtcga
     1981 gcgcgcgggt ctgccgcttg atctcgtgct ccagcgcgtc gcggacgttg cggaacgagt
     2041 tcaggttctt gatctcgacc ttggtcccgt actcggtctg cccgcgcggc cggagcgaga
     2101 tgttggcgtc gcagcgcagc gagccctcct ccatgttgcc gtcgcacacc ccgaggtaga
     2161 cgacgatggc gcgcacggcc cggagatacg cggcggcctc gtcggggctg cgcaggtcgg
     2221 gcttggagac gatctccatc agcggcacgc cggcccggtt gtagtccacg ctgctcgact
     2281 gggcggtctc gagcgcaccc tcgtggagca gcttgcccac gtcctcctcg aggtgcacac
     2341 gctggatgcc gatctcccgc gggcccggtc ccgtgtcgat caccagccgg ccgtgctccg
     2401 ccagcggctc ctcatattgg ctaatctggt agttcttcgg catgtccggg tagtagtagt
     2461 gcttccgcgc aaaccggttg tgcgcgttga tcccgcagcc gatcgcgagc gcggtcatga
     2521 tgccgaactc caccgcgcgg cggttgatga acgggagcga gccgggcata ccctggcaga
     2581 ccgggcaggt ggcggtgttg ggcggggcac cgaacaccgc ccggcagccg cagaacatct
     2641 tggtcacggt cgacagctga gcgtgaactt ccaggccgac tattgtttcg tagcgggcgg
     2701 cgccggtcat gtcgagagcg cggggcgacg gagatgccag tcggtgacag tctggtaggc
     2761 gtggaccgcg cgcagcaggg tggcctcgtc gaaggcgggc gcgatgagct gcagcccgat
     2821 cggcagcccg gcctg
//
LOCUS       DATMCV010000489         2048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_492872, whole genome shotgun sequence.
ACCESSION   DATMCV010000489 DATMCV010000000
VERSION     DATMCV010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492872"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1755)
                     /locus_tag="VNU03_11290"
     CDS             complement(172..1755)
                     /locus_tag="VNU03_11290"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP75095.1"
                     /translation="MLRRPDVDEVMEYSRALGFDLTPMEARMIQARMMDTIATLETFD
                     EMRVEERRPPLRFTDRDPGRRPSAAEDPLGAFITRCRVSGAASGPLQGKTVALKDHIA
                     LAGVPMTFSSHMMDGYVPDFDATVVTRLLDAGATITGKLKMEEFSWGGPGLSGVGDYG
                     RPLNPHNREHVTGGSSSGSGAAVAGGMVDIALGGDQGGSIRLPAAWCGIVGLMPTHGL
                     VPHSGVFGLEPTIDYVGPMARTVEDIAVSLQVMAGRDGLDPRQADVPATLPPYPAALA
                     RGVKGLRIGLLDEGFGVPGGDRAVDEAVMEAVRTLERAGARVERVSVPLHTQALPALL
                     PIYLEGGKRMYDTHFGGTGAKTHYPSSLISIFGRLKQSHAREFSPNLKLNLLQGYYLQ
                     RNYGGRLYAKAQNVRPTFVAQYDRVFAGVDVLAMPTIPMTAPRWQEPRDHQDAMELTM
                     LGGKRSLDLGPVIANTCPFNYTGHPAISVPCAKIGGLPVGLMLVAPYFREDTLIQAAA
                     AYQQSVDWAGLISAPRADG"
BASE COUNT          271 a          731 c          747 g          299 t
ORIGIN      
        1 cgcagcctgg ccgagcccga tgccgtgctc ggcgggctta acgggccggc cgcggtctcg
       61 gagcgctggc ggccttggcg ctcctacgtc gcgctctgtc aggcgactgc ggcacgagcc
      121 gccgcggagt ccgccccgcg cgggcgacgg gcctgaccgc tcgcgccccg ctcagccgtc
      181 ggcgcgcggc gcgctgatca acccggccca gtccaccgac tgctggtacg ccgccgcggc
      241 ctggatcagc gtgtcctcgc ggaagtaggg cgccaccagc atcagcccga ccggcagccc
      301 gccgatcttg gcgcacggca cgctgatggc cgggtggccg gtgtagttga acgggcacgt
      361 gttcgcgatc accgggccga ggtcgaggct gcgcttgccg cccagcatgg tcagctccat
      421 cgcgtcctgg tggtcgcgcg gctcctgcca gcgcggcgcg gtcatcggga tcgtcggcat
      481 cgcgagcacg tccactccgg cgaagacgcg atcgtactgg gccacgaagg tgggccgcac
      541 gttctgggcc ttggcgtaga ggcgcccgcc gtagttgcgc tgcaggtagt agccctggag
      601 gaggttcagc ttgaggttcg gcgagaactc gcgcgcgtgg ctctgcttga ggcgcccgaa
      661 gatcgagatc agcgaggacg ggtagtgggt cttggcaccg gtgccgccga agtgggtgtc
      721 gtacatccgc ttgcccccct cgaggtagat cggcagcagc gcggggagcg cctgggtgtg
      781 gagcggcacc gacactcgct cgacccgggc gcccgcgcgc tcgagggtgc gcaccgcctc
      841 catgaccgcc tcgtccaccg cgcggtcccc gccgggcacc ccgaagccct cgtcgagcag
      901 gccgatgcgc agccccttca ctccgcgcgc gagcgcggcg ggatagggtg gcagggtcgc
      961 ggggacgtcg gcctggcgcg gatcgaggcc gtcgcgcccg gccatgacct ggagcgacac
     1021 cgcgatgtcc tccacggtgc gggccatcgg ccccacgtag tcgatggtgg gctcgagccc
     1081 gaacacgccg gagtgcggga ccaggccgtg ggtcggcatg agccccacga tgccgcacca
     1141 cgcggcgggc aggcggatcg atccgccctg atcgccgccc agcgcgatgt cgaccatccc
     1201 gcccgccacc gccgcgcccg agcccgacga cgagccgccg gtgacgtgct cgcggttgtg
     1261 cgggttgagc gggcgaccgt agtcccccac tccggagagg cccgggcccc cccacgagaa
     1321 ctcctccatc ttgagcttgc cggtgatggt ggcgcccgcg tcgagcaggc gcgtgaccac
     1381 ggtcgcgtcg aagtccggca cgtagccgtc catcatgtgc gagctgaagg tcatcggcac
     1441 gccggcgagc gcgatgtgat ccttgagcgc gacggtcttg ccctggagcg ggccgctggc
     1501 cgcgcccgac acccggcagc gtgtgatgaa ggcgccgagc gggtcctcgg cggcggacgg
     1561 gcggcggccc gggtcgcggt cggtgaagcg cagcggcggg cgccgctcct ccacccgcat
     1621 ctcgtcgaag gtctcgagcg tggcgatggt gtccatcatg cgcgcctgga tcatgcgcgc
     1681 ctccatcggg gtcaggtcga atcccagggc acggctgtac tccatcacct cgtcgacgtc
     1741 cggtcgcctc agcattggcc tcggtcctcc gatcgggata gggcgttgcg gctgcatgat
     1801 gccatggact atagtgggcc gggcccgcgg cgtgtcgtgc ggccagtgga ctttctggag
     1861 aacgatggtc accgtgagcg ctgagacgtc catatcgtcc tcgctggacg agacctggac
     1921 ggcgctcggc cgtcggaata cgtacttcta cttccccggc atcgcccccg cgcccggcaa
     1981 gggcggcggc ccgctgaccc acgcgctcga gctgcccatc gtggaccggc aggagcaggc
     2041 cgccacgc
//
LOCUS       DATMCV010000490         3276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_760078, whole genome shotgun sequence.
ACCESSION   DATMCV010000490 DATMCV010000000
VERSION     DATMCV010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760078"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VNU03_11295"
     CDS             complement(<1..659)
                     /locus_tag="VNU03_11295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP75096.1"
                     /translation="MDFGAAIFFTDYSIGPPALGRALEERGFESLWAPEHSHIPLSRA
                     SAFPAGGELPKKYYDVMDPFVTLAAAAAATTRLRVATGICLVVQRDPIQTAKAVASLD
                     QVSGGRFLFGIGAGWNAEEMADHGTEFKSRFEVMRERVEAMRLIWTKSKPEYAGEYVK
                     FPPMMTWPKPVQKPHPPVLVGGAFPYGARRALAYGDGWVPHARRPAYGDVLGKLPEFR
                     A"
     gene            703..1734
                     /locus_tag="VNU03_11300"
     CDS             703..1734
                     /locus_tag="VNU03_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWP75097.1"
                     /translation="MSRQALLTTVVGSYPQPDWLVDRALLSKGVPRVRQREIWRVAEP
                     WLEQAQDDATILAIRDMERAGIDIVSDGEIRRESYSNRFATALDGVDVETPGVIRNRV
                     GQETVVPRVVGRVRRMRPVELRDMQFLRRHTDRPAKITLPGPFTMAQQAKNEFYEDME
                     ALAMDLAAAVNEEARDLQAAGADLIQLDEPWLRNDPEGAKRYAVPAINRALEGLTVPT
                     ALHLCFGYAAVVRHQKPTGYSFLPQLAATTAQQISIEAAQPRLDLGVLADLAGKTIIL
                     GVIDLADPAVETMDQVTARIRAGLARVTPDRLVLAPDCGMKYLPRDTAFGKLRALVDG
                     AHAVRAQLA"
     gene            1800..2642
                     /locus_tag="VNU03_11305"
     CDS             1800..2642
                     /locus_tag="VNU03_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP75098.1"
                     /translation="MATPVLVLDDPLPGVRRLTLNRPEKRNALSNELRGAILAALRTA
                     DADATVKVSVLRGAGPCFSSGYDLSADNTKDQPFYSPGGPGQWARHVVEGWFTIWDLA
                     KPVIAQVHGYCLAGGSELAACCDLVYVAEDARIGYPPTRLMSPPDNQFHPFLMGMRTA
                     MEMYLTGDAISGTEAAAKGFANRAVPVAKLEKTVLTVAERIALVPTELAQINKRAVHR
                     AMEVMGLRAAIRAGTELQALAFSTAASQAYRAQFKRDGSSVRDLLSQRDAAFGDYRER
                     ERKD"
     gene            complement(2639..>3276)
                     /locus_tag="VNU03_11310"
     CDS             complement(2639..>3276)
                     /locus_tag="VNU03_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP75099.1"
                     /translation="GFRVIAVQSDDLEADVAAMRRRGVDVGEIRDGGRRTPAGQELRW
                     RAAFPGPSVPLPIFFIQHLTPLEERRRQVAKAGDHPNGALRVERVYITVPDVVGTSRL
                     CARVLGLPEATIQRGAVIKADMAVFNLGPTGLTVAQPAAPGPAAEALARRGPGPFQVL
                     YRTSSMDAAARWMTDHGLPEPARGVRNTGEAAILVDPAHAGGTYVGFVGPA"
BASE COUNT          469 a         1246 c         1145 g          416 t
ORIGIN      
        1 atcgcgcgga actcgggcag cttgcccaac acgtcgccgt aggcgggccg gcgcgcgtgc
       61 ggcacccagc cgtcgccgta ggcgagggcc cgccgcgcgc cgtaggggaa cgcgccgccc
      121 accagcaccg gcgggtgcgg cttctgcacc ggcttgggcc acgtcatcat cggcgggaac
      181 ttcacgtact cgcccgcgta ctccggcttg gacttcgtcc agatgagccg catcgcctcc
      241 acccgctcgc gcatcacctc gaagcgcgac ttgaactcgg tgccgtggtc ggccatctcc
      301 tccgcgttcc agcccgcgcc gatcccgaac aggaagcgcc cgcccgagac ctggtcgagg
      361 ctcgcaaccg ccttggcggt ctggatcggg tcgcgctgga ccacgagaca gatcccggtc
      421 gccacccgga gccgcgtggt ggccgccgcg gcggccgcga gcgtgacgaa gggatccatc
      481 acgtcgtagt acttcttggg cagctcgccg cccgccggga acgcggaggc gcgcgagagc
      541 gggatgtgcg agtgctcggg cgcccagagc gactcgaagc cacgctcctc cagcgcccgg
      601 ccgagcgcgg gcgggcctat cgagtagtcg gtgaagaaga tcgccgcgcc gaaatccatg
      661 tgtcggccct cctggcccgc atccgtgcta gcatgccggc ccatgtctcg gcaagcgctg
      721 ctgaccacgg tggtcggaag ctacccgcag cccgactggc tcgtggaccg cgcgctgctc
      781 tcgaaaggcg tgccccgcgt ccgccagcgg gagatctggc gcgtggccga gccctggctc
      841 gagcaggccc aggacgacgc caccatcctg gcgatccgcg acatggagcg ggccggcatc
      901 gacatcgtca gcgacgggga gatccgacgc gagagctact cgaatcgctt cgccaccgcg
      961 ctggatggcg tggacgtcga gacacccggc gtgatccgca accgggtcgg ccaggagacg
     1021 gtggtgccgc gggtggtcgg ccgggtgcgg cggatgcggc cggtggagct gcgcgacatg
     1081 cagttcctcc gccgccacac cgaccggccg gccaagatca cgctgcccgg cccgttcacg
     1141 atggcccagc aggccaagaa cgagttctac gaggacatgg aagcgctcgc gatggacctg
     1201 gccgcggcgg tgaacgagga ggcacgtgac ctccaggcgg cgggcgccga tctcatccag
     1261 ctcgacgagc cgtggctgcg caacgatccg gaaggggcca agcgctacgc ggtgcccgcg
     1321 atcaaccggg cgctcgaggg cctcaccgtc ccgaccgcgc tccacctctg cttcggctac
     1381 gcggcggtgg tgcgccacca gaagccgacc ggctactcgt tcctgcccca gctcgccgcc
     1441 accaccgcgc agcagatctc gatcgaggcc gcccagcccc ggctcgacct gggcgtcctc
     1501 gccgacctcg cgggcaagac catcatcctg ggggtgatcg atctcgccga ccccgccgtc
     1561 gagacgatgg accaggtcac cgcgcgcatc cgggccggcc tcgcccgcgt gaccccggat
     1621 cgtctcgtcc tcgcgccgga ctgcgggatg aagtacctgc cgcgcgacac cgcgttcggc
     1681 aagctccgcg cgctcgtcga cggcgcccac gcggtccgcg cccagctcgc ctgagccgcg
     1741 ggcgcgagct ctcccccggg cgttgcgccc accgcgccgc ctggggcatg ctcggctcca
     1801 tggccacccc cgtgctcgtg ctcgacgatc cgctccccgg agtccgccgc ctcaccctga
     1861 accgcccgga gaagcgcaac gcgctgagca acgagctgcg cggcgccatc ctcgccgcgc
     1921 tgaggaccgc ggacgcggac gcgacggtca aggtctccgt cctgcgcggg gcgggaccct
     1981 gcttctcgtc cggctacgat ctctccgccg acaacaccaa ggaccagccc ttctactccc
     2041 cgggcggccc cgggcagtgg gcccgccacg tggtggaggg ctggttcacg atctgggacc
     2101 tggccaagcc ggtcatcgcg caggtgcacg gctactgcct ggccggcggc agcgagctgg
     2161 ccgcgtgctg cgacctggtc tacgtggcgg aggacgcgcg gatcggctac ccgccgaccc
     2221 gactcatgag cccgcccgac aaccagttcc acccgttcct gatgggcatg cgcaccgcga
     2281 tggagatgta cctcaccggc gacgcgatca gcggcaccga ggcggcggcc aagggcttcg
     2341 ccaaccgggc ggtgcccgtg gcaaagctcg agaagacggt gctgacggtg gccgagcgca
     2401 tcgcgctggt gcccaccgag ctcgcccaga tcaacaagcg cgcggtccac cgggcgatgg
     2461 aggtcatggg gctgcgcgcg gccatccgcg cgggcaccga gctgcaggcg ctcgcgttct
     2521 cgaccgcggc gagccaggcc taccgcgccc agttcaagcg cgacggcagc agcgtgcgcg
     2581 atctcctgag ccagcgcgac gccgccttcg gcgactaccg ggagcgcgag cggaaggact
     2641 aggcggggcc gacgaaaccc acgtacgtgc cgcccgcgtg cgcgggatcc accaggatgg
     2701 ccgcctcgcc ggtgttgcgc acgccgcgcg ccggctccgg caggccgtgg tcggtcatcc
     2761 accgcgccgc cgcgtccatg ctgctggtgc ggtagagcac ctggaacggc ccgggcccgc
     2821 ggcgggcgag cgcctcggcg gcgggtcccg gcgccgcggg ctgggccacc gtgagcccgg
     2881 tcggccccag gttaaacacc gccatgtccg ccttgatgac cgcgccccgc tggatcgtcg
     2941 cctcgggcag tccgagcacc cgggcgcaga gccgggacgt gccgaccacg tcgggcaccg
     3001 tgatgtagac gcgctcgacc cgcagcgccc cgttcggatg gtcgcccgcc ttcgcgacct
     3061 gccgccggcg ctcctcgagc ggcgtcaggt gctggatgaa gaagatgggc agcgggacgg
     3121 aggggccggg gaacgcggcg cgccagcgca gctcctgccc cgccggggtg cgccgacccc
     3181 cgtcgcggat ctcccccacg tccaccccgc gccggcgcat ggccgccacg tccgcctcga
     3241 ggtcgtcgct ctgcaccgcg atgacgcgga acccgc
//
LOCUS       DATMCV010000491         5675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_428760, whole genome shotgun sequence.
ACCESSION   DATMCV010000491 DATMCV010000000
VERSION     DATMCV010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428760"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VNU03_11315"
     CDS             complement(<1..340)
                     /locus_tag="VNU03_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP75100.1"
                     /translation="MAAGGPTRRVVMVGATGAGKSTLARRLADGLGVDFVELDALFWG
                     PGWAPAEPAAFRARVETATAGPAWVVAGNYGAVRDLLWARADTIVWLGYSLPLVLGRL
                     TARTLRRAITR"
     gene            complement(349..1200)
                     /locus_tag="VNU03_11320"
     CDS             complement(349..1200)
                     /locus_tag="VNU03_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75101.1"
                     /translation="MGRLDGKVIVVTGASRGIGADVARLFASEGGRVICAARTVQEGQ
                     HPLAGSLESTVAAIHEAGGDAHAVGANIAEPAECERLIREARQRYGPIDVLVNNAALT
                     YYVPVKDYPVGKWLRSWAVNFQAPFVLSQLVLADMVPRRSGAIVNISSGSAIGPGRGP
                     YPDPAVGARGGTCYGAEKAALERFTQGLASEVFQYGISVTSVAPSQVVPTPGTVYHRL
                     VSGLDDPRGEPPILMARAALLLATEPVERVSGRVTYSQQILREFGWIEAARGRGVDSR
                     GSGYSEI"
     gene            complement(1283..2587)
                     /locus_tag="VNU03_11325"
     CDS             complement(1283..2587)
                     /locus_tag="VNU03_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWP75102.1"
                     /translation="MFTTQGTSTRSVLTGIAILLTGCLALAGAAQAEVETTPIAMAVD
                     PATFQGEGPILASWSADSSRYAARYSEATWTETFGQPAEAPTATVQTASFTPAPTLER
                     SVVILPAAKPLAAYRVHVNEHVRFFLDRFQTGYRRAVVEGWLVRSGRYLPMILEVFRQ
                     KGLPEDLVFTVMIESGFNPVAVSQAGAKGLWQFMAPTARLYGLRVDGWLDERLDPEKS
                     TVAAANYLRDLYAQFGSWDLAQAAYNTGDGKIQQVIERTGSRDFWALRKSPLLPDETK
                     NFVPAIHAATLIGRQPERYGFSVVPEEPLQYEQVMVPKGSRLVRLAALSGLTLQDLES
                     LNPELRQKQTPPDMPYGLKVPLGTSHAVLAAVDLDAAAPRKIAPAPGTGVHVVQAGDT
                     VWRIAKRYGISAKQVVTWNGLDRPDRIFPGERLRVGALATAS"
     gene            2847..4052
                     /locus_tag="VNU03_11330"
     CDS             2847..4052
                     /locus_tag="VNU03_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP75103.1"
                     /translation="MRRETPAPDPELTPVGPGTPGGEYLRRFWQPVAFARDLDGPPRR
                     VRILGEDLVVFRDRSARVGVLHLHCAHRGTSLEFGIPIERGLRCCYHGWVFDVDGRCL
                     ETPGEPAGSRLCERVWQGAYPVHEFGGLVFAYLGPPDRVPAFPHYDSFDVPGHVLMPG
                     AEFTLPCHWLQVKDNSMDPVHTAFLHALSSGYQFTPAFGVVPELDWLTTEAGMVYIAT
                     RRVNDLVWVRVCDFMPPNVHQFTREIEEAAEPKPASRPVIIRWAVPNDDTHTTNFELA
                     QVDPAWGLTAEEVAQPGFGQSGDRSYDARQRFPADYDAQSSQRTVAVHAVEHLASTDR
                     GVIMLRRIVRDGIRAVQAGQDPWGTHWPEGKIVPTYTQDLVLRLPPAPSLEADHRLLR
                     ETGRRVVTG"
     gene            4065..>5675
                     /locus_tag="VNU03_11335"
     CDS             4065..>5675
                     /locus_tag="VNU03_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP75104.1"
                     /translation="MWIVRIALDRPYTFVVLALLILIVSPVVIARTPTDIFPAINIPV
                     IAVAWQYSGLNTEELEGRITTSYERVLTTTVDNIQRIESTTVNGQAIIKVYLQPNARL
                     DTANAQITAISQTVLRQYPPGIGPPLIINFSASSVPILQLALSGEGLAEQQLFDLGVN
                     FLRPQLVTIPGVAIPYPFGGKQRQVMVDLNTRMLQAKGLAPNDVLNTINLQNLILPSG
                     TAKIGQFEYYVNLNASPRTVPELADLPIKVVGNSTIYLRDVANVRDGFSVQTNIVRRD
                     GRRGTLLTVLKAGDASTLDVVDNIRAAIPRVATTLPPELKVDLLADQSIFVRGAISGV
                     IHEAIIAACLTALMILLFLGSWRSTLIIAVSIPLSILTSVIVLSFLRETINIMTLGGL
                     ALAVGILVDDATVTIENIERFLEDGRELRDAIFEGAAQIAVPALVSTLCICIVFLPMF
                     FLSGVARYLFVPLAEAVVFAMLASYLLSRTLVPTLAMYLLRPRDHHPAPTRNPLARFQ
                     RAFERGFERLRAAYRGLLIRLISRQGAFV"
BASE COUNT          826 a         2056 c         1941 g          852 t
ORIGIN      
        1 cgcgggtgat cgcgcggcgg agggtgcggg cggtcagccg gccgagcacg aggggcaggg
       61 agtagccgag ccagacgatg gtgtcggcgc gggcccacag cagatcgcgg accgcgccgt
      121 agttgccggc caccacccac gccgggcccg cggtcgccgt ctccacccgc gcccggaacg
      181 cggccggctc ggccggcgcc cagcccgggc cccagaagag tgcgtccagc tccacgaagt
      241 cgaccccgag cccatcggcc agccgccggg cgagcgtcga cttgcccgcc ccggtcgcgc
      301 cgaccatcac cacccgtcgc gtcgggccgc cggcggccac ggcgccggct agatctccga
      361 gtagccgctg ccgcgggagt cgacgccccg gccgcgcgcg gcctcgatcc agccgaactc
      421 gcggaggatc tgctggctgt aggtcacccg gccgctcacg cgctcgaccg gctcggtggc
      481 gagcaggagc gcggcgcgcg ccatcaggat cggcggctcg ccccgcgggt cgtccagccc
      541 gctgacgagg cggtgataca cggtgcccgg agtgggcacg acctgcgagg gggcgaccga
      601 ggtgaccgag atgccgtact ggaagacctc ggaggcgagg ccctgggtga agcgctcgag
      661 cgcggccttc tcggcgccgt agcaggtgcc gccgcgcgcg cccaccgccg gatccgggta
      721 gggcccgcgc ccgggtccga tggcggagcc cgaggagatg ttcacgatcg ccccgctccg
      781 gcgcgggacc atgtcggcga gcaccagctg gctcaggacg aacggggcct ggaagttcac
      841 cgcccacgag cggagccact tgccgaccgg gtagtccttc accgggacgt agtaggtcag
      901 cgcggcgttg ttgaccagca cgtcgatcgg gccgtagcgc tggcgcgcct cgcggatcag
      961 ccgctcgcac tcggcgggct cggcgatgtt ggcgcccacc gcgtgggcgt cgccgcccgc
     1021 ctcgtggatg gccgcgaccg tgctctcgag cgagcccgcg aggggatgct ggccttcctg
     1081 gacggtgcgg gccgcgcaga tcacgcggcc gccttcggat gcgaagagcc gggcgacgtc
     1141 ggcgccgatg ccgcggctcg caccggtgac cacgatgacc ttgccgtcga gtcggcccat
     1201 gagttcctcc gggccgctat tgtccacgac ggcgccgccc ggtcaaccgg gtgaagcccg
     1261 gtcgaccggg cggccccccg agctaggacg cggtggcgag ggcgccgacc cggagtcgct
     1321 cgcccgggaa gatgcggtcc gggcgatcca ggccgttcca cgtcacgacc tgcttggcgg
     1381 agatgccgta ccgcttcgcg atgcgccaca ccgtgtcgcc ggcctgcacc acgtgaacgc
     1441 cggtgcccgg ggcgggagcg atcttgcgcg gggccgccgc gtccaggtcc accgccgcca
     1501 gcaccgcgtg gctggtcccg agcggcacct tgagcccgta gggcatgtcg ggcggcgtct
     1561 gcttctggcg cagctccggg ttcagggatt cgagatcctg gagcgtgagc ccggagaggg
     1621 cggccagccg tacgagccgg ctgcccttgg gaaccatgac ctgctcgtac tgcagcggct
     1681 cctcgggtac cacgctgaag ccgtaccgct cgggctgacg gccgatgagg gtggcggcgt
     1741 gaatcgcggg cacgaagttc ttggtctcgt cgggcaggag cgggctcttg cggagcgccc
     1801 agaagtcgcg cgagccggtg cgctcgatca cctgctggat cttcccgtcg cccgtgttgt
     1861 aggcggcctg ggccagatcc cacgagccga actgagcgta gaggtcacgc aggtagttgg
     1921 cggcggccac cgtggacttc tcggggtcga gccgctcgtc cagccagccg tccacccgaa
     1981 ggccgtagag acgggcggtc ggggccatga actgccagag gcccttggcg ccggcctggg
     2041 agacggccac cgggttgaag ccgctctcga tcatcacggt gaacacgagg tcctcgggga
     2101 gacccttctg ccggaagacc tcgaggatca tcggcaggta gcgcccggag cgcacgagcc
     2161 agccctccac gaccgcgcgg cggtagccgg tctggaagcg atcgaggaag aaccggacgt
     2221 gctcgttcac gtgcacgcga tacgccgcca gcggcttggc cgccggcagg atcaccacgc
     2281 tccgctccag agtgggcgcc ggcgtgaagc tggcggtctg gacggtggcg gtcggtgctt
     2341 cggccggctg gccgaacgtc tcggtccagg tggcttccga gtacctggcc gcgtatctgg
     2401 acgagtcggc ggaccaggat gcgaggatcg gcccctctcc ctggaaggtg gcgggatcca
     2461 cggccatggc gatcggagtg gtctcgacct cagcctgggc ggccccggcg agggccaggc
     2521 agccggtgag gaggatggca atgccggtca gaacggaccg cgttgacgtg ccctgtgttg
     2581 tgaacatgaa tcgcatggat agcaaggccc aagccacaaa taacaagcta tttttattga
     2641 tgtttttagg atgacagtac cacagaaacc ccctctctgg gtacggggcc acccaccatg
     2701 ggagatttgc gcgccgcgca gattctgcgc tgatccagga gcgacccgga agcctcgacg
     2761 cgacttgacg cggcgtcgac ctggccccat gctgtgcccc accaatgtcc ccggcgcgag
     2821 catccggtag tccgtacgac gggtacgtgc gtcgtgagac tccggccccg gatcccgagc
     2881 tcaccccggt tggtcccggc acgccgggcg gcgaatacct gaggcgattc tggcagccgg
     2941 tcgcgtttgc gcgcgatctg gacgggccgc cgcgccgcgt gcgcatcctc ggcgaggatc
     3001 tggtggtctt ccgcgaccgg agcgcacgcg tcggcgtgct gcacctgcac tgcgcgcatc
     3061 gcggcacctc gctcgagttc ggcattccca tcgagcgcgg cctgcgctgc tgctatcacg
     3121 gctgggtgtt cgacgtcgac gggcgctgtc tcgagacgcc cggcgagccc gcggggagcc
     3181 ggctctgcga gcgcgtgtgg cagggcgcgt acccggtcca cgagttcggc gggctcgtgt
     3241 tcgcgtacct gggtccgccc gaccgggttc ccgcgttccc gcactacgat tccttcgacg
     3301 tgcccggcca cgtcctgatg ccgggggccg agttcaccct gccgtgccac tggctgcagg
     3361 tgaaggacaa cagcatggac ccggtgcaca ccgcgttcct gcacgcgctg tcgagcggct
     3421 accagttcac acccgccttc ggggtcgtgc cggagctgga ctggctcacc accgaggcgg
     3481 ggatggtcta catcgcgacc cgccgggtca atgatctggt ctgggtgcgc gtctgcgact
     3541 tcatgccgcc gaacgtgcac cagttcacgc gcgagatcga ggaggcggcc gagcccaagc
     3601 cggcgagccg gccggtgatc atccgctggg cggtccccaa cgacgacacc cacaccacca
     3661 acttcgagct ggcccaggtg gaccccgcgt ggggcctcac cgcggaggag gtcgcccagc
     3721 ccggcttcgg ccagtccggc gaccggtcct acgacgcgcg gcagcgcttt ccggccgact
     3781 acgacgccca gtcgagccag cggacggtcg cggtccacgc ggtcgagcac ctcgcctcga
     3841 ccgatcgcgg ggtgatcatg ctgcggcgga tcgtgcgcga cggcatccgc gcggtgcagg
     3901 cggggcagga cccgtggggc acccactggc cggagggcaa gatcgtcccg acctacaccc
     3961 aggacctcgt tctgcgcctg ccgcccgccc ccagcctgga ggcggaccat cggctgctcc
     4021 gcgagaccgg gcgccgggtc gtcaccggct gagccgcccg ccccatgtgg atcgtccgga
     4081 tcgcgctgga ccggccctac accttcgtcg tcctcgcgct cctcatcctg atcgtgagcc
     4141 cggtggtgat cgcgcgcacc cccaccgaca tcttccccgc gatcaacatc ccggtcatcg
     4201 cggtggcctg gcagtacagt gggctcaaca ccgaggagct ggaagggcgg atcaccacct
     4261 cctacgagcg cgtgctcacc accacggtgg acaacatcca gcgcatcgag tccaccaccg
     4321 tgaacggcca ggccatcatc aaggtgtacc ttcagccgaa cgcacggctc gacaccgcca
     4381 atgcccagat caccgcgatc tcgcagaccg tgctccgcca gtacccgccc gggatcggcc
     4441 cgccgctgat catcaacttc agcgcctcca gcgtgccgat cctccagctc gcgctctccg
     4501 gggaaggcct cgccgagcag cagctcttcg acctgggggt caacttcctg cgtccccagc
     4561 tcgtgaccat ccccggcgtg gccatcccgt acccgttcgg gggcaagcag cgccaggtca
     4621 tggtggacct caatacccgg atgctgcagg ccaaggggct ggctcctaac gacgtgctca
     4681 acaccatcaa cctccagaac ctgatcctgc cctccggcac cgccaagatc ggccagttcg
     4741 agtactacgt gaacctcaac gcgtcccccc gcacggtgcc ggagctggcc gacctgccga
     4801 tcaaggtggt gggcaactcc accatctacc tccgcgacgt cgccaacgtg cgcgacggct
     4861 tcagcgtgca gaccaacatc gtgcgccgcg acggccgccg cggcaccctg ctcaccgtgc
     4921 tcaaggcggg cgacgcctcc accctggacg tggtggacaa catccgggcc gcgatcccgc
     4981 gggtggccac caccctgccg cccgagctga aggtcgacct gctcgcggac cagtccatct
     5041 tcgtgcgggg ggcgatctcg ggcgtgatcc acgaggcgat catcgcggcg tgtctgaccg
     5101 ccctgatgat cctgctcttc ctgggcagct ggcggagcac cctgatcatc gcggtctcga
     5161 tcccgctctc gatcctgacc tcggtgatcg tgctgagctt cctgcgcgag accatcaaca
     5221 tcatgaccct gggcggtctc gccctcgcgg tcggcatcct ggtcgacgac gccacagtga
     5281 ccatcgagaa catcgagcgc ttcctcgagg atgggcggga gctgcgcgac gcgatcttcg
     5341 agggcgcggc ccagatcgcg gtgccggccc tggtctccac cctctgcatc tgcatcgtgt
     5401 tcctgcccat gttcttcctg agcggggtgg cccgctacct cttcgtgccg ctcgcggagg
     5461 cggtggtctt cgcgatgctc gcgtcctacc tgctctcgcg caccctcgtg cccaccctcg
     5521 cgatgtacct gctgcgcccg cgcgaccacc atccggcccc cacccgcaac ccgctcgcgc
     5581 ggttccagcg cgccttcgag cgcggtttcg agcggctgcg cgcggcctac cggggcctgc
     5641 tcatccggct gatctcgcgc cagggcgcct tcgtg
//
LOCUS       DATMCV010000492         2891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_893972, whole genome shotgun sequence.
ACCESSION   DATMCV010000492 DATMCV010000000
VERSION     DATMCV010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893972"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..405
                     /locus_tag="VNU03_11340"
     CDS             67..405
                     /locus_tag="VNU03_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75105.1"
                     /translation="MDELMGELVRLGFLLTLWAFGIWVGGASVAAWVAWEKGRDPAAW
                     FVMGFFLSPLVALIALSAVPARHLARVRAAQRARGVARSRPAESSRMTTPVTAAARLV
                     GGVRERKVNA"
     gene            413..1363
                     /locus_tag="VNU03_11345"
     CDS             413..1363
                     /locus_tag="VNU03_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWP75106.1"
                     /translation="MNPWAVVLAGGEGTRLRPLIRRVLGDERPKQYVPLLGPRSLLRQ
                     TLDRVALRMPVSRTVVVTVRAHTGYIAQEFTGGAEPPYVLVQPSDRGTAAGILHAAHW
                     IRRRDPEATIAFFPSDHFILGEATFMAHVADVVRAVEGQGDRVALVGAQPTSPETDYG
                     WMQPGAAIDGSDGMLRTVREFREKPSEARVQLGLATGCLWNTAIMVARAETLIALGAE
                     ALPEMHARLDRVGAFADSDEEPAALHQAYALMSPTGFGRAVLEHHAERLAVSALPRLT
                     WCDLGSPSRVLAVLARMRVRPAWAEAPAWSEVIDQPAIPA"
     gene            1378..2232
                     /locus_tag="VNU03_11350"
     CDS             1378..2232
                     /locus_tag="VNU03_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWP75107.1"
                     /translation="MASTLWTGSISFGLVTIPVSLVTAEVHEELDFTLLDRRDDSPIG
                     YQKINKRTGKPVPADRIVRGFEYQDGRYVVLSDADLRRASPERTQRIDVLSFVDRAEI
                     PPVYFDRPYYLEPARGGERGYALFREALRRSDRVAVATVVVKTRQHLAAIVVQDGVLV
                     LDLLRYAVELRDPSRLRVPGPGLKGVSEREVKMAERLIDEMTEAWKAERYRDEYHDEL
                     LAFIKKRGRAGKLAAAPEAEEEPARRPRADVIDIADLLQKSLAGKGGRARSKPAEERR
                     RPRRRKTA"
     gene            2235..2777
                     /locus_tag="VNU03_11355"
     CDS             2235..2777
                     /locus_tag="VNU03_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HWP75108.1"
                     /translation="MSLTLYRRKRDFAQTPEPRGRARKSAARLSFVIQRHAATRLHYD
                     FRLELDGVLKSWAVPKEPKAVVGDKRLAVHVEDHPLEYGRFHGVIPEGHYGAGRVEIW
                     DRGWWEPVGDPHADYRKGSLKFVLHGRRLRGGWALVRMRPRGDDPRDRGKENWLLIKE
                     HDKTRAARTRAAAPARTRAR"
BASE COUNT          383 a         1002 c         1105 g          401 t
ORIGIN      
        1 agcctgatcg tcattctgtt cctgctgggc gtcgggcggt aggcccggcc gccgcgaggt
       61 gccgccatgg acgagctgat gggcgagctc gtgagactgg gattcctcct cacgctctgg
      121 gcattcggga tctgggtcgg cggcgcctcc gtggcggcct gggtcgcctg ggaaaagggt
      181 cgcgatccgg cggcctggtt cgtgatgggt ttcttcctgt ccccgctcgt tgccctgatc
      241 gcgctgagcg cggtgccggc acggcacctg gctcgggtgc gggcggcgca acgggcacgg
      301 ggggtcgcgc ggtcgcggcc ggcggagtcg tcgcgcatga cgactccggt gaccgcggcc
      361 gcgcggctcg tcggtggggt gcgtgagcgc aaggtcaacg cgtagcgccg ccgtgaaccc
      421 gtgggccgtg gtgctcgcgg ggggtgaggg aacgcggctg cggcccctga tccggcgcgt
      481 gctcggcgac gagcggccca agcagtacgt tccgctgctc gggccccgga gcctgctgcg
      541 ccagaccctc gatcgggtgg cgctccgcat gccggtctcc cgcaccgtgg tggtgacggt
      601 gcgcgcccac accggctaca tcgcccagga gttcaccggg ggcgcggagc caccgtacgt
      661 cctggtgcag ccgagcgacc ggggcactgc ggccggcatc ctccacgccg cgcactggat
      721 ccggcggcgc gatccggagg ccaccatcgc cttctttccg tccgaccact tcatcctggg
      781 cgaggcgacc ttcatggctc acgtcgcgga cgtcgtccgg gcggtggaag gccagggtga
      841 tcgggtcgca ctggtcgggg cccagcccac ctcgcccgag accgactacg gctggatgca
      901 gccgggcgcg gcgatcgacg ggtccgacgg gatgctccga acggtccgtg agttccgcga
      961 gaagccgtcg gaggcccggg tgcagctcgg gctggccacc ggctgtctct ggaacaccgc
     1021 aatcatggtg gcccgggccg agacgctgat cgcgctggga gccgaagccc tgcccgagat
     1081 gcacgcccgg ctcgaccggg tcggcgcctt cgcggactcg gacgaggagc cggccgcgct
     1141 gcatcaggcg tatgcgctga tgagcccgac gggcttcggg cgcgcggtgc tcgagcacca
     1201 tgcggagcgc ctcgcggtct cggcccttcc ccggttgacc tggtgcgacc tcggcagccc
     1261 gagccgcgtg ctcgcggtgc tcgcgcgcat gcgggtgcgc ccggcgtggg cggaggcgcc
     1321 ggcgtggtcc gaggtgattg accagccggc gatcccggcg tagcctccgc tccgaccatg
     1381 gcgtcgaccc tgtggaccgg cagcatcagc ttcggcctcg tcacgatccc ggtctcgctg
     1441 gtgaccgcgg aggtgcacga ggagctggac ttcaccctgc tcgaccggcg cgacgactca
     1501 ccgatcggct accagaagat caacaagcgc accggcaagc cggtgcccgc ggatcggatc
     1561 gtgcgcggct tcgagtacca ggacgggcgc tatgtggtgc tgagcgacgc ggatctgcgc
     1621 cgggcgagcc cggaacgcac ccagcgcatc gacgtcctgt ccttcgtcga tcgggccgag
     1681 atccccccgg tctacttcga tcggccctac tatctcgagc cggcccgcgg cggcgagcgg
     1741 ggctacgccc tgttccgcga ggccctgcgg cgcagcgacc gggtggcggt cgcgacggtg
     1801 gtggtgaaga cgcgtcagca cctcgcggcg atcgtggtgc aggacggggt gctggtgctc
     1861 gacctgctgc gctacgccgt cgagctgcgt gacccgtcgc gcctgcgcgt ccccggtccc
     1921 gggctcaagg gggtctccga gcgcgaggtc aagatggccg agcggctgat cgacgagatg
     1981 acggaggcgt ggaaggccga gcgctaccgc gacgagtatc acgacgagct gctggccttc
     2041 atcaagaagc gcggccgcgc cgggaagctg gccgcggcgc ccgaggcgga ggaggagccg
     2101 gcgcgccgcc cgcgggccga cgtgatcgac atcgccgacc tcctgcagaa gagcctcgcg
     2161 ggcaagggcg ggcgggcccg gagcaagccg gccgaggagc ggcgccggcc ccgccggcgg
     2221 aagaccgcct gagcgtgagc ctcacgctct atcgccggaa gcgcgacttc gcccagacgc
     2281 ccgagccgcg cgggcgggcg cggaagtccg ccgcgcgcct gtccttcgtg atccagcgcc
     2341 acgcggccac ccgcctgcac tacgacttcc gcctggagct cgacggcgtt ctcaagagct
     2401 gggcggtgcc gaaggagccc aaggcggtgg tgggggacaa gcggctcgcg gtgcacgtcg
     2461 aggaccatcc gctcgagtac gggcgcttcc acggcgtgat cccggaaggt cactacggcg
     2521 cgggtcgggt cgagatctgg gaccgcggct ggtgggagcc ggtgggtgac ccgcatgcgg
     2581 actaccgcaa gggctcgctc aagttcgtcc tgcacggccg gcggctgcgc ggcggctggg
     2641 cgctggtgcg gatgcgcccg cggggcgacg atccgagaga ccgcggcaag gaaaactggc
     2701 tcctcatcaa ggagcacgac aagacgcgcg ccgcccgcac gcgcgccgcc gccccggccc
     2761 ggacccgcgc gcgatgacgc tgccgggctg ggtcgcgccc cagctcgcga cgctggtggg
     2821 ggccgcgccc gacggggacg aatgggtgca cgagatcaag ctcgacggct atcgcgtgct
     2881 ggcccggatc g
//
LOCUS       DATMCV010000493         4795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_689556, whole genome shotgun sequence.
ACCESSION   DATMCV010000493 DATMCV010000000
VERSION     DATMCV010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689556"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1796)
                     /locus_tag="VNU03_11360"
     CDS             complement(192..1796)
                     /locus_tag="VNU03_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP75109.1"
                     /translation="MKPGAPITATLTELMGERAARDPDTPYFHLFDEAVPYGRLWRES
                     ARYAAGLRRAGIDRGDKVCLIYPTCAEFFYTFFGALRLGAIPVPLYPTLGVEATAGIF
                     RDSEAKAVATIGWFRQGVDESQAAAANIRHVLEPSDLEVDGPAPPFPSAATDDVAFIQ
                     YTSGSTGRPRGVVLSHANVVRTVEFMAEAAQLTRDDVVVSWLPLYHDMGLIGCAFTPP
                     WNGAPLHLLPPDLKNPRVWLEMVTRVGATFTVSPDFGYRNCVRNIGDTAGLDLSSLKQ
                     ALSGAEPVRLSTIEAFESKFGVQNLITPCYGLAEATLAVAIWPRQTPLRLDSSGKFLS
                     VGQPCRGVRVRIMEGPDEVKAGVEAEICVASPGVMQGYYNNPEATAQVLSPDGWLRTG
                     DLGFLDAEGYLYVTGRLKDVIILGGENVIPSDIEEIVDHVPGVRYSAAVGLESERTGT
                     QRLHVVAEVREQAGDAETLSRLVREIVQRVREGRGHRPARVLLVRPGTIPKTSSGKIQ
                     RSRLSEMIARDELAASLLHASGAPRG"
     gene            complement(1845..2561)
                     /locus_tag="VNU03_11365"
     CDS             complement(1845..2561)
                     /locus_tag="VNU03_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWP75110.1"
                     /translation="MPDASQPVPIQRVLVVTAHPDDSEFGAGGTVAKLVREGKHVSYC
                     IVTNGNKGSSDRSMTPERIARIREEEQRAAARVLGVETIDFLGFPDCEVEDTRETRMA
                     VTAAIRRHRPDRLIIQNPNRTKNLGASHRDHRIVAGIALDCVYPLARDHMAFPELAAQ
                     GLEPHKVREVYLMWWDKPEVVVDISDTIDLKIKALACHASQFKDFTAVEARVRERGAE
                     LGKLGSHAYAETFDLISMDR"
     gene            2616..2828
                     /locus_tag="VNU03_11370"
     CDS             2616..2828
                     /locus_tag="VNU03_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75111.1"
                     /translation="MSTRSIGVALLWLLLAGFLVIGALPNQTASIRLAGLSLLWWYGG
                     LLAPVLGVVVTIRCLADPPPPPPRGE"
     gene            2825..4309
                     /locus_tag="VNU03_11375"
     CDS             2825..4309
                     /locus_tag="VNU03_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75112.1"
                     /translation="MRSASRVLAVWLTPAALLAIPAALCGRAADGLWLGLAWTLVPLI
                     VLGLQANPRPAGAGRDERGSPFPVVTLLVTVGVLLWANVALAGDVAVWLGVPRWQGIV
                     VTAVAGWLLTAWRPLRRTAPLLLSIALVAATAPLLQVAWAVGLGPIAAWERVATQAAF
                     RFPPASPWVTTGRSLAAAHGRAPLVFDEVHRLTAPAGGTLRARTLDGTRPAEFEWTLT
                     PGQSVTLRAGDELVPGAALRLRFEADKRVPGAPPSGMAWAETPPRRWLDRVGLLVTLL
                     VGAAALLRAGLTLRLSRNRIGFVAGGLLLAFAWAQAWAVYAVLQSPDVFLGGVTLERL
                     LELPGAALFAEPWRRTLQAFLLVAGLAAFLASSVALRERLGALDPTGGGEIGSDPGLW
                     AGLFGGAALASLWPVDPWALTLLALGGAGCALGPATLYPPPPGRAALATTAGVVGLLV
                     FATFAAAQLRGAGGALLGAAIGYPALVGAPAGALVLWLGRGRAG"
     gene            4306..>4795
                     /locus_tag="VNU03_11380"
     CDS             4306..>4795
                     /locus_tag="VNU03_11380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75113.1"
                     /translation="MTGDPRLVTPTLAACLGITAGAHIAVYLLNATLPLHLVALGGSK
                     TQVGLLFSVSGMVSMVLRPLVGGWIDRYGFRPVMLPGAVVLVATLLILPLAATPAAFI
                     ALMGGIGLGNGLISTGAGVLAAQASPPTRRGEALGIYYVATSLAFSVGPPVGFALYAR
                     GGI"
BASE COUNT          537 a         1719 c         1794 g          745 t
ORIGIN      
        1 tggccggagg gactgtaggg gtattgcggg tcgatccggt agggggtcac cacgtggtcg
       61 ctcgcccaga gcgagtcgta gccgagcgcc tcgatccggc gcgcgaaggt gagaacgttg
      121 tcgcgcgtgc cgacgggacc gtacatcggc aggtgacagc ccaccttcat gatcgggtcc
      181 gcacgagagg cctagccccg gggggcgccc gaggcgtgga ggaggctcgc ggcgagctcg
      241 tcgcgcgcga tcatctcgga cagccgcgag cgctggatct tcccggacga cgtcttcggg
      301 atggtaccgg gacggaccag caggacgcgc gccgggcgat ggccccgtcc ctcgcgcacc
      361 cgctggacga tctcgcgcac gagccgcgag agggtctccg cgtcgccggc ctgctcgcgt
      421 acctcggcca ccacgtgcag gcgctgggtg ccggtgcgct cgctctcgag gccgaccgcg
      481 gccgagtagc gcaccccggg gacgtggtcg acgatctcct cgatgtcgga cgggatcacg
      541 ttctccccgc cgaggatgat cacgtccttg agccgcccgg tcacgtagag gtagccctcg
      601 gcgtcgagga agccgaggtc gccggtgcgg agccagccgt cgggcgagag cacctgggcg
      661 gtcgcctccg ggttgttgta gtagccctgc atgaccccgg ggctggccac gcagatctcg
      721 gcctccacgc ccgccttgac ctcgtcgggg ccttccatga tccgcacccg cacgccccgg
      781 cagggctgcc caaccgagag gaacttgccg gagctgtcca gccgcagcgg cgtctggcgc
      841 ggccagatgg cgaccgccag ggtggcctcg gccaggccgt agcacggggt gatgaggttc
      901 tgcacgccga acttcgactc gaaggcctcg atggtgctga gccggaccgg ctccgcgccg
      961 gagagcgcct gcttgagcga ggagagatcg agcccggcgg tgtcgccgat gttgcgcacg
     1021 cagttgcggt agccgaagtc gggcgacacc gtgaaggtgg cgcccacccg ggtgaccatc
     1081 tcgagccaga cccgcgggtt cttcagatcg ggcggcagca ggtggagcgg cgccccgttc
     1141 cagggcgggg tgaacgcgca gccgatgagc cccatgtcgt gatagagcgg cagccacgac
     1201 accaccacgt cgtcgcgcgt gagctgcgcg gcctcggcca tgaactccac cgtgcgcacc
     1261 acgttcgcgt ggctcagcac cacgccgcgc ggccggccgg tgctccccga cgtgtactgg
     1321 atgaaggcca cgtcatcggt cgccgcggag gggaacggcg gcgcggggcc gtccacctcc
     1381 agatcgctcg gctcgagcac gtgccggatg ttggccgcgg cggcctgcga ctcgtccacg
     1441 ccctggcgga accacccgat ggtggcgacc gccttggcct cggagtcgcg gaagatgccg
     1501 gcggtggcct cgaccccgag ggtggggtac agcggcaccg ggatcgcgcc cagtcgcagg
     1561 gcgccgaaga acgtgtagaa gaactcggcg caggtgggat agatcaggca caccttgtcg
     1621 ccgcggtcga tgccggcgcg gcggagcccg gccgcgtagc gggcgctctc ccgccagagc
     1681 cggccgtacg gcaccgcttc gtcgaagagg tggaagtacg gggtgtcggg gtcccgggcg
     1741 gcccgctccc ccatcagctc ggtcagggtg gccgtgatgg gcgctcctgg cttcacggcg
     1801 gcattctagc caatgcgccg ccgacgggga agcattctcg gcgactagcg gtccatcgag
     1861 atcaggtcga aggtctcggc gtaggcgtgg ctcccgagct tgccgagctc ggccccgcgc
     1921 tcgcgaaccc gcgcctcgac ggccgtgaag tccttgaact ggctcgcgtg gcaggcgagg
     1981 gccttgatct tgagatcgat ggtgtcggag atgtcgacca ccacctccgg cttgtcccac
     2041 cacatcaggt agacctcgcg gaccttgtgc ggctcgagcc cctgcgcggc cagctccggg
     2101 aaggccatgt gatcgcgggc gaggggatac acgcagtcga gcgcgatgcc ggccacgatg
     2161 cggtggtcgc ggtgggaggc acccaggttc ttggtgcggt tcgggttctg gatgatcagc
     2221 cggtccggcc ggtgccgccg gatcgccgcg gtcaccgcca tccgggtctc gcgggtgtcc
     2281 tcgacctcgc agtcggggaa cccgaggaag tcgatcgtct cgacacccag gacccgcgcg
     2341 gcggcgcgct gttcctcctc gcggatccgg gcgatgcgct ccggggtcat gctgcggtcg
     2401 ctcgagccct tgttgccgtt ggtgacgatg cagtaggaga cgtgcttgcc ctcgcgcacg
     2461 agcttggcga cggtcccacc ggctccgaac tcggagtcgt cggggtgggc ggtcacgacc
     2521 aagacgcgct ggatggggac gggctgactc gcatcgggca tgagaagggc tcctttcgtg
     2581 gtcgctatgt taccaccccc gctataatgc ggccggtgag cacccgctcg atcggtgtcg
     2641 ccctgctctg gctcctgctc gccgggttcc tcgtcatcgg cgcgctgccc aaccagacgg
     2701 cgtcgatccg cctcgccggc ctctcgctgc tctggtggta cggagggctg ctcgcgccgg
     2761 tgctgggggt ggtggtgacg atccgctgcc tcgccgatcc accgcccccg ccgccgcgcg
     2821 gcgaatgagg tcggcctctc gcgtcctcgc cgtctggctg acgccggcgg cgctgctggc
     2881 gatcccggcc gcgctgtgcg gccgcgccgc ggacggcctc tggctcgggc tcgcgtggac
     2941 tctcgtgccg ctcatcgtcc tcggtctcca ggcgaacccg cggcccgcgg gcgccggccg
     3001 cgacgagcgt gggtcgccct tcccggtggt gacgctcctg gtgacggtcg gcgtcctgct
     3061 ctgggcgaac gtcgcgctcg cgggcgatgt cgcggtctgg ctgggcgtcc cgcgctggca
     3121 gggcatcgtg gtcaccgcgg tcgcgggctg gctgctcacc gcgtggcggc cgctgcggcg
     3181 gaccgctccg ctgctgctct cgatcgccct cgtggccgcg acggcgccgc tgctgcaggt
     3241 ggcctgggcg gtcgggctcg gccccatcgc ggcgtgggag cgcgtggcca cgcaggccgc
     3301 attccgcttt ccgcccgcga gcccctgggt gacgacggga cggagcctcg ccgccgcgca
     3361 cggccgcgcg ccgctcgtct tcgacgaggt gcatcgcctg accgccccgg ccggcggcac
     3421 gctgcgggcg cgcaccctcg acggtacccg cccggccgag ttcgagtgga cgctgactcc
     3481 gggccagtcg gtcacgctcc gcgcggggga cgagctggtg cccggcgcgg cgctgcgatt
     3541 gcgcttcgag gccgacaagc gcgtgcccgg ggctcccccg tcgggcatgg catgggccga
     3601 gaccccgccg cggcgctggc tcgatcgcgt cggcctgctc gtcacgttgc tggtgggagc
     3661 ggccgcgctc ctccgggccg ggctgaccct ccgcctctcc cgcaaccgca tcggcttcgt
     3721 cgcgggcggg ctgctgttgg ccttcgcgtg ggcgcaggcg tgggcggtgt atgcggtgct
     3781 gcaatcaccc gatgtcttcc tgggcggcgt caccctcgag cgtctcctcg agctgccggg
     3841 cgccgcgctc ttcgccgagc cctggcgccg cacgctccag gcgttcctgc tggtggccgg
     3901 cctcgccgcg ttcctcgcgt cgagcgtggc gctgcgcgag cggctgggcg cgctcgatcc
     3961 gacggggggc ggggagatcg ggagcgatcc gggcctctgg gccggcctct tcggcggcgc
     4021 ggccctcgcg agcctctggc cggtggatcc gtgggccctt accctgctgg ccctgggcgg
     4081 cgccggctgt gcgctcgggc ccgccacgct ctacccgccg cctcccggcc gggcggcgct
     4141 ggcgaccacc gcgggcgtcg tcggcctcct cgtgttcgcg accttcgcgg cggcgcagct
     4201 gcgcggcgcc ggcggcgcgc tcctgggggc cgcgatcggc tacccggcgc tggtgggcgc
     4261 ccccgccggc gcgctcgtgc tctggctcgg gcgcggacgc gcggggtgac gggggatccg
     4321 cggctcgtca ccccgacgct cgccgcctgc ctggggatca ccgccggcgc ccacatcgcg
     4381 gtctacctcc tgaacgccac gctgccgctg catctggtcg cgctcggcgg cagcaagacg
     4441 caggtggggc tgctcttctc ggtgagcggg atggtctcga tggtgctgcg cccgctcgtg
     4501 ggcggctgga tcgatcgcta cggcttccgc ccggtgatgc tgccgggggc ggtggtcctc
     4561 gtggccacgc tcctgatcct gccgctggcc gcgaccccgg cggccttcat cgcgctcatg
     4621 ggtggcatcg ggctcggcaa cgggctgatc tcgaccgggg cgggtgtcct ggccgcgcag
     4681 gcgagccccc cgacccggcg cggggaagcg ctcggcatct actacgtcgc cacgtcgctc
     4741 gcgttctcgg tgggcccgcc ggtcggcttc gcgctctacg cgcgcggcgg catcc
//
LOCUS       DATMCV010000494         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_689639, whole genome shotgun sequence.
ACCESSION   DATMCV010000494 DATMCV010000000
VERSION     DATMCV010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689639"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VNU03_11385"
     CDS             complement(<1..516)
                     /locus_tag="VNU03_11385"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP75114.1"
                     /translation="MIRLVTFDFWDTLVTDAPANLRAQRTLRVAAIRRALRDAGELVS
                     EAEAEDVHERSGVLLAERFWNLNRDPSPEEQLRLVLDTVEPGVAARLAPPAFAAALAG
                     YTEPVLAYPPELCPNAAQAVRELAARGVAVGIVSNTGRTPGAVLRRVLERYGLLAYFG
                     AVSYSDEVGVRK"
     gene            complement(513..1280)
                     /locus_tag="VNU03_11390"
     CDS             complement(513..1280)
                     /locus_tag="VNU03_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP75115.1"
                     /translation="MTGFVRVEREGACAWVTLDRPPLNLIVPEMVEGIRSTFEALRRD
                     PGVRAAVITGAGRATTAGMQLQVLRELTPARAKAFIASLHEAIGLVHDAPFPTVCMVN
                     GACLGAGFELAMACDLRTAATEAVLGLPEIRVGIPSVIEAALLPGLVGPGRAAEILLT
                     GEPVTARQALDWGLVNRVTPLGELRAVTSELVGRILDCAPTAVRLQKELMVRWRNTDQ
                     RTAVESGINAFAQSFTTDEPREAMDAYLGKRKPRFAP"
     gene            complement(1314..1628)
                     /locus_tag="VNU03_11395"
     CDS             complement(1314..1628)
                     /locus_tag="VNU03_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWP75116.1"
                     /translation="MTEISPQELKARLDRHESLVLLDVREDWETALCRLANATHIPIE
                     EIEFRTAELDQADDIVVYCHHGVRSAAVANFLRQQGFKAVNLEGGVDQWARAIDPTMK
                     RY"
     gene            complement(1659..>2297)
                     /locus_tag="VNU03_11400"
     CDS             complement(1659..>2297)
                     /locus_tag="VNU03_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010268647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75117.1"
                     /translation="LIVEPRLLLLDEPLSNLDAVLRAQMRLELGRLHRDVGITTIYVT
                     HDQAEALALSTRIAVLHQGRIVQEGKPEEIYWRPRNRFVAEFVGAANLLPVRVTEVRD
                     VGVMVETAGGSRVPVASGGHPWTVGATGLLCLRPEALRIEESERAAGGIRGKVSGHVF
                     EGSRQLYDVAIPGGAVRVEMLTSAQLGKGFKPGDDIKIEISPETSVLLPDES"
BASE COUNT          328 a          839 c          823 g          307 t
ORIGIN      
        1 cttccggacg ccgacctcgt cggaatagga gaccgcgccg aagtacgcga gcaggccgta
       61 gcgctcgagg acccggcgca ggaccgcgcc gggggtgcgg ccggtgttgg acacgatgcc
      121 gaccgcgacg ccgcgcgcgg ccagctcgcg caccgcctgg gccgcattcg ggcagagctc
      181 cgggggatag gccagcaccg gctcggtgta gcccgcgagc gcggcggcga atgcgggcgg
      241 agcgagtcgc gcggccacgc ccggctccac ggtgtcgagc acgaggcgca gctgctcctc
      301 ggggctcggg tcccggttga ggttccagaa gcgctcggcg agcagcaccc ccgagcgctc
      361 gtggacgtcc tccgcctcgg cctcgctcac cagctccccc gcgtcgcgga gcgcccgccg
      421 gatggcggcg acccggagcg tgcgctgcgc acgcaggttg gcgggcgcgt cggtgacgag
      481 ggtgtcccag aagtcgaacg tgacgaggcg gatcacggag cgaagcgggg cttgcgctta
      541 ccgagatagg cgtccatcgc ctcgcgcggc tcgtcggtcg tgaacgactg ggcaaaggcg
      601 ttgatcccgg actcgaccgc ggtgcgctgg tcggtattgc gccaccggac catcagctcc
      661 ttctgcagcc gcaccgcggt gggcgcgcag tcgaggatgc ggccgaccag ctccgaggtc
      721 accgcccgca gctcgccgag cggggtcacc cggttgacga ggccccagtc gagcgcctgc
      781 cgcgcggtga ccggctcgcc ggtcaacagg atctcggccg cgcgccccgg gcccaccagg
      841 ccgggcagca gcgcggcctc gatcaccgac gggatgccga cgcggatctc gggcaggccc
      901 agcaccgcct cggtggcggc ggtgcgcagg tcgcacgcca tggccagctc gaagcccgcg
      961 ccgaggcagg ccccgttgac catgcacacg gtcggaaagg gcgcgtcgtg caccaggccg
     1021 atcgcctcgt gcaggctcgc gatgaaggcc ttcgcgcggg cgggcgtgag ctcgcgcagc
     1081 acctgcagct gcatgcccgc ggtggtcgcg cgcccggccc cggtgatcac cgccgcgcgc
     1141 acgcccggat cgcggcgcag cgcctcgaac gtgctccgga tcccctccac catctcgggc
     1201 acgatcaggt tgagcggggg acggtcgagg gtgacccacg cgcacgcgcc ctcgcgctcg
     1261 acccggacga agccggtcat cgataccgcg tgctcatcaa tagcgcttcc ccatcaatag
     1321 cgcttcatcg tcggatcgat ggcgcgcgcc cactggtcca cgccgccctc caggttgacc
     1381 gccttgaagc cctgctggcg gaggaagttg gccaccgcgg cgctgcgcac cccgtggtgg
     1441 cagtacacga cgatgtcgtc ggcctggtcc agctccgcgg tgcggaactc gatctcctcg
     1501 atcgggatgt gggtggcgtt ggcgaggcgg cagagcgcgg tctcccagtc ctcgcgtacg
     1561 tcgagcagca cgagggactc gtggcgatcg agccgcgcct tcagctcctg ggggctgatc
     1621 tcggtcatgg cggggtctcc cggggcgaag atagcatgtc aggactcgtc gggcagcagc
     1681 accgaggtct cgggcgagat ctcgatcttg atgtcgtcgc ccggcttgaa tcccttgccg
     1741 agctgggccg aggtcagcat ctccacccgc accgcaccgc ccggaatcgc gacgtcgtaa
     1801 agctgccgcg atccctcgaa cacgtggccg gacaccttgc cgcggatgcc gccggccgcg
     1861 cgctccgact cctcgatccg cagcgcctcc ggccgcaggc agagcaggcc ggtcgccccc
     1921 accgtccacg gatgcccccc cgaggccacc ggcacgcggg agccgcccgc ggtctccacc
     1981 atcacgccga cgtcgcgcac ctcggtcacc cgcaccggca gcaggttggc cgcgcccacg
     2041 aactcggcca cgaagcggtt ccgcggccgc cagtagatct cctccggctt gccctcctgg
     2101 acgatgcggc cctggtggag gaccgcgatg cgggtggaca gggccagggc ctcggcctgg
     2161 tcgtgggtga cgtagatcgt ggtgatgccc acgtcacgat ggagccgccc cagctcgagg
     2221 cgcatctggg cgcgcagcac cgcgtccagg ttcgagagcg gctcgtcgag caggagcagg
     2281 cgcggctcga cgatcag
//
LOCUS       DATMCV010000495         2035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_225072, whole genome shotgun sequence.
ACCESSION   DATMCV010000495 DATMCV010000000
VERSION     DATMCV010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225072"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VNU03_11405"
     CDS             <1..861
                     /locus_tag="VNU03_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HWP75118.1"
                     /translation="RIFLAVRHGDRRPITDIFVHTPEIPPAAQWCLFLRNHDELTLEM
                     VSDEERRHLYYAYGSDPRMRLNLGIRRRLAPLLENDRRKIELLTSLLFTLPGSPILYY
                     GDEIGMGDNIYLGDRNGVRTPMQWTGDRNAGFSRADTARLYLPLIVDAVYGYQSINVE
                     AQQRTPSSLLNWMKRLIAVRRKTPVFGRGTLHFLRPANESVLAHVREHEGEILVAVHN
                     LAGSAQPVELDLHAWRGYTPIEMLGDTRFPTIGDLPYFISLAPYGYYWFRLQPPLSAE
                     PLYGIEASPL"
     gene            836..>2035
                     /locus_tag="VNU03_11410"
     CDS             836..>2035
                     /locus_tag="VNU03_11410"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75119.1"
                     /translation="MASRPPRSDPGAIVALLSGPGRSDLAQYLVRQRWFAAKTRGIGA
                     LAVRDWATLDEDAPLVLLLLDVDGDRYHVPVTVAPAAAPASTMASLDGAVVIDAHDDP
                     RFARRVLAAIAAGLRLDGRAGRFAFHPTPDFPFPPDPDALPARRHTGEQSNTSVILGS
                     GLVLKTLRRPQPGLNPDLEITRFLTTRTPFRHVPRLAGWVEYESAEGLADLAVLQEFV
                     ANRGDGWKHVVTTLREGLAAGAGRLASDPLIEDMRRLGAITGGLHAALASDGADPDFA
                     PEPIGREDVARWAADIRRDVEAADVLAPADTSRDGVERTLASLEALVGTVKIRSHGDY
                     HLGQVLKTEDGFAVIDFEGEPARPLVERRARQSALRDVAGMLRSLDYAAHAVAFEVPA
                     AERAAALA"
BASE COUNT          269 a          813 c          675 g          278 t
ORIGIN      
        1 cgcatcttcc tggccgtgcg ccacggcgac cgccggccca tcaccgacat cttcgtgcac
       61 acccccgaga tcccgcccgc ggcccagtgg tgcctcttcc tccgcaacca cgacgagctc
      121 accctcgaga tggtcagtga cgaggagcgg cgccatctct actacgccta cggctccgac
      181 ccgcgcatgc gcctgaacct cgggataagg cgtcgcttgg cgccgctgct cgagaacgac
      241 cggcggaaga tcgagctgct gacgagcctg ctcttcaccc tgcccggcag ccccatcctc
      301 tactacggcg acgagatcgg gatgggcgac aacatctacc tgggcgaccg caacggcgtc
      361 cgcaccccga tgcagtggac gggcgaccgc aacgcgggct tctcccgcgc cgacaccgcg
      421 cgcctctacc tgcccctgat cgtggacgcg gtctacggct accagtccat caacgtggag
      481 gcccagcagc gcaccccctc gtcgctgctg aactggatga agcgcctgat cgcggtgcgc
      541 cggaagacgc ccgtcttcgg ccgcggaacc ctccacttcc tccgcccggc caacgagagc
      601 gtgctggccc acgtgcgcga gcacgagggc gagatcctgg tcgcggtgca caacctggcc
      661 gggtccgccc agccggtgga gctcgacctg cacgcgtggc gcgggtacac cccgatcgag
      721 atgctgggcg acacccgctt ccccaccatc ggcgacctgc cctacttcat cagcctggcg
      781 ccctacggct actactggtt ccgtctgcag ccgccgctct cggccgagcc cctctatggc
      841 atcgaggcct ccccgctctg accccggcgc catcgtcgcg ctgctctcgg gccccggccg
      901 ctccgatctc gcccagtacc tggtccgcca gcgctggttc gcggccaaga cccgcgggat
      961 cggcgcgctc gcggtccgcg actgggccac gctcgacgag gacgcgccgc tcgtcctgct
     1021 gctcctcgac gtcgacggcg accgttatca cgtcccggtg accgtggccc cggcggccgc
     1081 gccggcgtcc acgatggcga gcctcgacgg cgccgtggtc atcgacgcgc acgacgaccc
     1141 gcgcttcgcc cggcgggtcc tcgccgcgat cgcggcgggc ctccgcctgg acggccgcgc
     1201 cgggcgcttc gcgttccacc ccaccccgga cttccccttc ccgcccgacc ccgacgcgct
     1261 cccggcgcgc cgccacaccg gcgagcagag caatacctcg gtgatcctgg gcagcgggct
     1321 cgtgctcaag accctccgcc ggccgcagcc cggcctcaac ccggatctgg agatcacgcg
     1381 cttcctcacg acccgcaccc cgttccgtca cgtcccgcgc ctggccggct gggtcgagta
     1441 cgagagcgcc gaggggctcg ccgacctcgc ggtgctccag gagttcgtgg cgaaccgggg
     1501 cgacgggtgg aagcacgtgg tgacgacgct ccgcgagggg ctcgcggccg gggccgggcg
     1561 cctcgcctcc gatccgctga tcgaggacat gcggcggctc ggcgcgatca ccggcggcct
     1621 ccacgccgcg ctggcctcgg acggggcgga cccggacttc gcgccggagc cgatcggccg
     1681 cgaggacgtc gcgcgctggg ccgccgacat ccgccgcgac gtggaggccg ccgacgtcct
     1741 cgcgcccgcc gacacgagcc gggacggcgt cgagcggacg ctcgcctcgc tcgaggcgct
     1801 cgtcggcacc gtgaagatcc ggagccacgg cgactatcac ctgggacagg tgctgaagac
     1861 cgaggacggc ttcgcggtga tcgacttcga gggcgagccc gcgcgccccc tggtggagcg
     1921 ccgggctcgg cagtcggccc tacgtgacgt ggccggcatg ttgcgctcgc tcgactacgc
     1981 ggcgcacgcg gtggccttcg aggtgcccgc ggccgagcgc gcggcggccc tggcc
//
LOCUS       DATMCV010000496         6366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_225164, whole genome shotgun sequence.
ACCESSION   DATMCV010000496 DATMCV010000000
VERSION     DATMCV010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225164"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            548..1522
                     /locus_tag="VNU03_11415"
     CDS             548..1522
                     /locus_tag="VNU03_11415"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75120.1"
                     /translation="MDRRAFIGTVAGGLLAAPLAVEAQPAPKVARLGVLLFSTPAAEP
                     NLPALLAGLRDLGYVEGRNIVLEYRGAEGRSERVGGLAHQIVALKPDLIVVLGGDLVP
                     FVKGATSTIPVVMLTSQDPVEAGVVASFARPGGNLTGVAFVSSETAGKRLQFLKEAVP
                     SLTRVAVLWNPDHPDGEYRDTEAAARRLSIHVQSLEVRRPEEFDGAFEAATRARPEAL
                     MVVSSRFMNLNRSRVLEFVGKQRIPLVTGWGPWAHAGSFMSYGPDLDALVRRAATHVD
                     KILKGAKPADLPVEQPTKFELVINLKTAKALGLTIPPSLLQRADQVIE"
     gene            1577..1804
                     /locus_tag="VNU03_11420"
     CDS             1577..1804
                     /locus_tag="VNU03_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWP75121.1"
                     /translation="MLEAAGRGAEHFEGYTSHGNRHTFASRLVMAGVDLRTVQQLRGW
                     QRTPWYPRRRLIVGAACNILIRLRGGVAEPG"
     gene            1781..1872
                     /locus_tag="VNU03_11425"
     tRNA            1781..1872
                     /locus_tag="VNU03_11425"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1817..1819,aa:Ser,seq:cga)
     gene            1910..1999
                     /locus_tag="VNU03_11430"
     tRNA            1910..1999
                     /locus_tag="VNU03_11430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1944..1946,aa:Ser,seq:gga)
     gene            2015..2279
                     /gene="ffs"
                     /locus_tag="VNU03_11435"
     ncRNA           2015..2279
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VNU03_11435"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            2283..4097
                     /gene="dnaX"
                     /locus_tag="VNU03_11440"
     CDS             2283..4097
                     /gene="dnaX"
                     /locus_tag="VNU03_11440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWP75122.1"
                     /translation="MTYQVLARKWRPQEFDAVVGQDPVTRTLRNALSSGRVAHAYLFT
                     GPRGVGKTTTARLLAKALACTARTGPEACGACPSCQDFVSGAPVDVMEIDAASNTSVD
                     DIRTLRENVKYSPARGRFKVYIVDEVHMLSGAAFNAFLKTLEEPPAHVVFILATTDPK
                     KIPATVLSRCQRFDFRPIAPEQLTLSLTRILEQEGVPFEPGALPVLIRAAEGSLRDAL
                     SLLDTAIAYGEGKLDEAGVARLLGSSSPVHVRGFVGALLSRDGAAALEAIDRAATGGE
                     DLGALCREAIEMARRLLVLKVASGTPFADLTPAEAAELRAAAEPVSADELIYVLRAFL
                     DADGEMRRSPHPRVELEIATVRATRRPQPQAIETLITKLEEAEARLRQMPTSTGGAAP
                     TRPVVVQATLLDARPAPGATPPPRAAAAPPPVAVSASTTTPAPASAPAASSAASASAP
                     AASARSLRPPDDEPLVETAAADIEAAEAVPAPASGLDTAWERVVTDLMKRKALLGSVL
                     QHATPLRVEDGVLTLALQASGFHREMLNDRGNKELINEAVQRHVASARRFELDPNGVA
                     PAGGGGARNHPAVQAALSAFQGEVVAVRPRTPEEGEKP"
     gene            4094..4408
                     /locus_tag="VNU03_11445"
     CDS             4094..4408
                     /locus_tag="VNU03_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWP75123.1"
                     /translation="MKGFGNIMKEAQKLQAQMEQLQAEAAKKTVEATAGGGMVTVVAN
                     GKQEVVSIKIDREVINPEDGQMLEDLVLAATNEALRKSKEMVQGEMSKITAGLKIPGL
                     GM"
     gene            4408..5010
                     /gene="recR"
                     /locus_tag="VNU03_11450"
     CDS             4408..5010
                     /gene="recR"
                     /locus_tag="VNU03_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWP75124.1"
                     /translation="MAYYPEPVARLIEALQRLPGIGPKTAQRLTFFLLKRPAPEVAAL
                     GEALTQLKQRIVHCSVCFNVTEQDPCRICTEPQRDQRTLCVVEEPNDLLALERTGEFR
                     GRYHVLLGALSPLDGIGPEDLRVRELLARLDNDHVEEVILATNPSVEGEATAIYLARL
                     LKPLGMRITRIARGLPVGGDLEYADEVTLSKAMEGRREMG"
     gene            5071..5145
                     /locus_tag="VNU03_11455"
     tRNA            5071..5145
                     /locus_tag="VNU03_11455"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5103..5105,aa:Asn,seq:gtt)
     gene            5174..>6366
                     /locus_tag="VNU03_11460"
     CDS             5174..>6366
                     /locus_tag="VNU03_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWP75125.1"
                     /translation="MPPLTDARKKSLRREMEALLGAGGVLSDPDELMVYESDGLTLFR
                     ALADFVVFPRTAEDVSVLVKLASREGIPFVARGAGTGLSGGCLPTEGGLVISLMRMNR
                     VLEVDYDNQMAVVEPGLVNLHLSWAVGPQGFYYAPDPSSQQACTIGGNIANNSGGPHT
                     LKYGVTTNHVLGLEVVLPDGELMWIGGKTRDAQGYDLVGLFVGSEGTFGIATKIVVRI
                     LKKPQAVKTVLAVFDQVDQASEAVSAIIARGLVPAAMEMIDQLTIEAVEDAFGCGYPR
                     DAAAALLIELDGLAAGMEAQAERVVQACRDCGARDVRAARDEAERALLWKGRKSAFGA
                     YGRISPAYMVMDGVIPRTKLPYVLGRVNAIVAAHGLRVGNVFHAGDGNLHPNILYDPR
                     KPGEEA"
BASE COUNT          990 a         2181 c         2212 g          983 t
ORIGIN      
        1 attcgtcggc ggtcattcga tggcgccttg gtcgaaaacg ccagttcgcg gcccgtttct
       61 tctttcggcc tacgcgcggt tcttcctaca tgccgatctc acggaggtcg tcctcactcg
      121 tcccggaagg accctgaaca gagctatcag cgaggaggcg ttgaagcgcc tccgcttcca
      181 gacggacccg cgttagctct ctcttcagtt cagcgttctc cttggccagg tccagtagtt
      241 ggagcataga atcctcctgc gtcccggaaa gcaagaggag ggccagattg cgcagggcgg
      301 caaaattgcc caggaagacc cgatacagag cagacacggc tacccgggcc ggagattctt
      361 acactgacgc gcccccataa cttcccgctt cgggctctta ctcagatagc gcgggtgtgg
      421 tggtctcccc gcccatgacc ggacgttcaa tgcgcctccc cgactcccac acgtgactcg
      481 ggtgtagtaa ggtcgctgcg agccgcccgc ccgtctttcc taaacggcgg caaagtgagg
      541 gtggctagtg gaccgacgag cgttcatcgg caccgttgct gggggcctcc tcgccgcgcc
      601 gctcgcggtt gaggcgcagc cggcccccaa agtcgcgcgc ctcggcgtgc tgctcttcag
      661 tacgccggcc gccgaaccta acctgcctgc cttgcttgcg ggcctgcgcg atctaggcta
      721 tgtcgagggc cggaacatcg tgctcgagta tcgtggcgcc gagggccgct cggagcgagt
      781 cggcggactt gcgcaccaga ttgtggcgtt gaagcccgat ctaatcgtgg ttctcggcgg
      841 agacttggtg ccgttcgtta agggtgcgac gagcacgatc ccggtcgtga tgctcaccag
      901 tcaagacccc gtcgaggcag gggtggtggc cagcttcgcc cggcccggcg gcaacctgac
      961 cggcgtagcc ttcgtttcat cagagacggc aggcaagcga cttcagttcc tcaaagaggc
     1021 agtgccgtca ctcacccgcg tcgcagtgct ctggaacccc gatcatcccg acggcgagta
     1081 tcgcgacacc gaggcggccg cgcgtcggct tagcatccac gttcaatccc tcgaagtcag
     1141 gcggcccgag gagttcgatg gggcctttga agcggccacg cgagcgcgtc ccgaggcgct
     1201 gatggtcgtc tcctcgcgat tcatgaacct caaccgctcc cgcgtcctcg agtttgtggg
     1261 caagcagcgg atccccctcg tgactggctg gggaccgtgg gcacatgcgg gaagcttcat
     1321 gagctacggg cctgacctcg atgccctcgt gagacgggcg gcgactcacg tggacaaaat
     1381 cctcaagggt gcgaagcccg ccgacctccc cgtggagcag ccgacaaagt tcgagttggt
     1441 gatcaacctc aagaccgcca aggccctcgg cctcacgatt cccccatctc tcctgcagcg
     1501 ggcggatcag gtgatcgagt gaccctcgag ccgcgccgac aagttcttcc cggcggcggt
     1561 ggagcgggcc aagcgggtgc tcgaggcggc tggccggggc gcggagcatt tcgaggggta
     1621 cacatcgcac ggcaaccggc acactttcgc ctcccggctc gtcatggctg gggtagactt
     1681 gcgcacggtt cagcaactga ggggttggca gaggactccg tggtatccga ggcggaggtt
     1741 gatcgtaggc gcggcgtgca acatactgat acggttacgc ggaggggtgg ccgagcccgg
     1801 ttgaaggcgt ccgactcgaa atcggatagg ggcgcaagtc cctcgggggt tcaaatcctc
     1861 tcccctccgc caatttgatc gcaccttcag gagtgccaga tagtgacgcg gaggggtggc
     1921 cgagtggccg aaggtgcggc actggaaatg ccgtgtacgg gcaactgtac cgtgggttcg
     1981 aatcccaccc cctccgccat atcttttcgg ttggccgtgc tagacgggga ggtagcggtg
     2041 ccctgtaacc cgcaatccgc tatagcgggg ttgaattccc tctcgagggc cggtcgtgtc
     2101 gaatgagccg tacgggacgg cgttgagggt tgggccccac gcaacgggat gcgtcgaacc
     2161 ccgccaggcc cggaagggag caacggtaag gcgctcctct cgtgtgccgt gggagtacct
     2221 gaccggagca ggtttcgtgc ggcgatcgcg ggacggtcgg ttcgattgag ggtgcacggt
     2281 ctttgacgta tcaggtcctg gcgcggaagt ggcggccgca ggagttcgac gcggtcgtgg
     2341 ggcaagaccc ggtcacccgc acgctccgca acgcgctcag ctcgggccgg gtggcccacg
     2401 cctacctctt caccggcccg cgcggggtcg gcaagaccac caccgcgcgg ctgctcgcca
     2461 aggcgctcgc ctgcaccgcg cgcaccgggc ccgaagcgtg cggcgcgtgc ccctcctgcc
     2521 aggacttcgt ctcgggggcg ccagtcgacg tgatggagat cgacgcggcc tccaacacga
     2581 gcgtggacga tatccggacg ctccgggaga acgtcaagta ttctcccgcg cgcggccgct
     2641 tcaaggtcta catcgtggac gaggtgcaca tgctctccgg ggcggcgttc aacgccttcc
     2701 tgaagacgct ggaggagccg cccgcccacg tggtgttcat cctggccacc acggatccga
     2761 agaagatccc ggccaccgtg ctgtcgcgtt gccagcgctt cgacttccgg ccgatcgccc
     2821 ccgagcagct caccctgagc ctcacccgca tcctcgagca ggagggcgtg cccttcgagc
     2881 cgggtgcgct gccggtgctg atccgggccg ccgagggcag cctgcgtgac gcgctctcgc
     2941 tgctcgacac cgcgatcgcc tacggtgaag gcaagctcga cgaggcgggc gtggcccgcc
     3001 tgctcggctc ctcctcgccg gtgcacgtgc gcggcttcgt gggcgcgctc ctctcccgcg
     3061 acggcgccgc cgccctcgag gcgatcgatc gcgcggccac cggcggcgag gacctgggcg
     3121 cgctctgccg cgaggccatc gagatggccc gccggctcct ggtgctgaag gtcgcctccg
     3181 gcacgccctt cgccgacctc acgcccgccg aggccgccga gctgcgcgcg gccgcggagc
     3241 cggtgagcgc cgacgagctg atctacgtgc tgcgcgcgtt cctcgatgcc gacggcgaga
     3301 tgcgccgctc gccccatccg cgggtcgagc tcgagatcgc gacggtgcgc gcgacgaggc
     3361 gcccgcagcc gcaggccatc gagacgctga tcaccaagct cgaggaggcg gaggcgcggc
     3421 tgcggcagat gcccacgtcg acgggcgggg ccgcgcccac tcgcccggtg gtggtgcagg
     3481 cgacgctgct cgacgcccgc ccggctcccg gcgcgacccc gccgccccgc gccgcggccg
     3541 ctccgccgcc agtggcggtg tcggcgagca cgacgacccc cgcacccgcg tcggcccccg
     3601 ccgcgtcgtc ggctgcgtcg gcctccgcgc ccgcggcgtc ggcccggagc ctgcggccgc
     3661 ccgacgacga gcccctcgtc gagaccgccg cggccgacat cgaggcggcc gaggcggtgc
     3721 cggcccccgc gagcggcctc gacaccgcgt gggagcgcgt ggtcaccgac ctgatgaagc
     3781 gcaaggcgct gctcggctcg gtgctgcagc acgcgacgcc gctgcgtgtg gaggacggcg
     3841 tgctcacgct cgcgctgcag gcgagtggct tccaccgcga gatgctgaac gatcggggca
     3901 acaaggagct gatcaacgaa gcggtgcagc gccacgtggc gtcggcgcgc cggttcgagc
     3961 tcgatcccaa tggcgtcgcg ccggccggcg gcggcggggc ccggaaccac ccggccgtgc
     4021 aggccgcgct gagcgcattc cagggagagg tggtcgcggt gcggccccgg accccggagg
     4081 aaggagagaa accgtgaaag gcttcggcaa catcatgaag gaggcgcaga agctccaggc
     4141 ccagatggag cagctccagg cggaggcggc caagaagacg gtggaggcca ccgcgggggg
     4201 cgggatggtc acggtggtgg ccaacggcaa gcaggaggtg gtgtcgatca agatcgatcg
     4261 cgaggtcatc aaccccgagg acgggcagat gctcgaggat ctggtgctcg cggccaccaa
     4321 cgaggcgctg cgcaagtcga aggagatggt gcagggggag atgagcaaga tcaccgccgg
     4381 actcaagatc cccgggctcg ggatgtaatg gcctattacc cggagccggt ggcgcgcctc
     4441 atcgaggcgc tccagcgcct gcccggcatc gggcccaaga ccgcccagcg tctgaccttc
     4501 ttcctcctca agcgtccggc ccccgaggtg gccgcgctcg gggaggcgct gacccagctc
     4561 aagcagcgca tcgtgcactg ctcggtctgc ttcaacgtga ccgagcagga tccctgtcgc
     4621 atctgcaccg agccgcagcg cgaccagcgg acgctctgcg tggtggagga gcccaacgac
     4681 ctgctcgcgc tggagcggac cggcgagttc cgcggccgct accacgtcct gctgggcgcg
     4741 ctctcccccc tcgacggcat cggccccgag gatctgagag tgcgcgagct gctggcccgg
     4801 ctcgacaacg accacgtcga ggaagtgatc ctggccacca acccgagcgt ggagggcgag
     4861 gccaccgcga tctacctggc ccggctgctg aagccgctcg gcatgcggat cacccgcatc
     4921 gcgcgggggc tgccggtggg cggtgacctc gagtacgccg acgaggtgac gctctccaag
     4981 gccatggaag gccggcgcga gatggggtag cggtgcttgg cgggcgtccg gccgtgtggt
     5041 agaaatgaac cggacggttc cctcctggat tccccgatag ctcaatggta gagcatccgg
     5101 ctgttaaccg gagggttgca ggttcgagtc ctgctcgggg agccactcct cctcccgatg
     5161 ctataatcgc cccgtgcccc ccctgaccga cgctcgcaag aagtccctcc gtcgcgagat
     5221 ggaagcgctc ctcggcgcgg gtggcgttct ctccgacccc gacgagctga tggtctacga
     5281 gtccgacggg ctcaccctct tccgggccct ggccgacttc gtggtgttcc cccgcaccgc
     5341 cgaggacgtg tcggtgctgg tcaagctggc cagccgcgag gggatcccgt tcgtggcccg
     5401 cggcgccggc accgggctct ccggcggctg cctgcccacc gagggcggcc tcgtcatctc
     5461 gctcatgcgg atgaaccggg tgctcgaggt ggactacgac aaccagatgg cggtggtgga
     5521 gccgggactg gtgaacctgc acctctcctg ggcggtcggc ccgcagggct tctactacgc
     5581 gcccgacccc tcgagccagc aggcgtgcac catcggcggc aacatcgcca acaactcggg
     5641 cggcccgcac accctgaagt acggggtgac gaccaaccac gtgctgggcc tcgaggtggt
     5701 gctgccggac ggcgagctga tgtggatcgg cggcaagacg cgcgacgcgc agggctacga
     5761 cctggtcggc ctcttcgtgg gctcggaggg caccttcggc atcgcgacca agatcgtcgt
     5821 ccgcatcctc aagaagccgc aggcggtgaa gacggtgctg gccgttttcg accaggtcga
     5881 ccaggcgtcc gaagccgtct ccgccatcat cgcgcgcggc ctcgtgcccg ccgcgatgga
     5941 gatgatcgat cagctcacca tcgaggcggt ggaggacgcg ttcggctgcg gctacccgcg
     6001 cgacgcggcg gccgcgctgc tgatcgagct ggacgggctc gcggccggca tggaggcgca
     6061 ggccgagcgg gtggttcagg cctgccgcga ctgcggcgcc cgtgacgtgc gcgccgcgcg
     6121 cgacgaggcc gagcgcgcgc tgctctggaa gggtcgcaag tcggccttcg gcgcctacgg
     6181 ccgcatctcg cccgcctaca tggtgatgga cggcgtcatc ccgcgcacca agctgcccta
     6241 cgtgctgggg cgcgtgaacg cgatcgtggc cgcccacggc ctgcgcgtcg gcaacgtctt
     6301 ccacgcgggc gacggcaatc tccacccgaa catcctctac gatccgcgca agccgggcga
     6361 ggaggc
//
LOCUS       DATMCV010000497         6796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_23947, whole genome shotgun sequence.
ACCESSION   DATMCV010000497 DATMCV010000000
VERSION     DATMCV010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23947"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VNU03_11465"
     CDS             complement(<1..1256)
                     /locus_tag="VNU03_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75126.1"
                     /translation="MSDDLLAPFPSRLDRRQFLTRAGLAGLGAGALVVGGRGRAHAQA
                     RVAYPDWIPASPNPKRGGVLTRASAWDPPVIDPRLTQSVGLYQFAGLVGSRLVRYVFP
                     DETSGPADLTLKGDLAESWQASPDFRVWTFKLRQGVKWHNLPPLNGREFVAADVKYCF
                     EAYAKEGAQSFTFQEIEAMETPDKYTVRIHLKTPNVFFPQNVAEPISVLFSREVLEED
                     GDLKKRMIGTGPFTLKEHTRKVRLVLARNPDYFDKGRPYVDEYVILSTPDAATRMAAF
                     RTGQSDIMWLASPSEVDTVKRTNANAVVHAYNNALTPFGIALAQDKPPFNDLRVRRAI
                     SMSIDRQKQVDTVFEGHGMAGWGVPYIYYQEKPPTLAQLGPWWQYRPAEARKLLAEAG
                     HPNGFETTLFYYEYFPQMSSQVQLVQ"
     gene            1404..2663
                     /locus_tag="VNU03_11470"
     CDS             1404..2663
                     /locus_tag="VNU03_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75127.1"
                     /translation="MGKVLGLLAVLALVAGPAPAETLGGVLAARSVGSPPSRLVLDQP
                     LATYQVLDDARDLIVVYLEEPAAAGRLQAARFERATRRWTVARLDWPVAADGSGAKAL
                     EPEWCRSGLGIERFPGGFLVRSHLNPSAECTVVLAPDLSVRAVLAGWPVAALADGRLV
                     YQRNQVHFAAVHPVALALLDARRPREIALYPPRPPGEIRRAHVDRLRSVYTAAWCNAH
                     NHPCDPERFDEQLSARVVADAGGDALAFVTAWDNTVGWSEAERWGRLEPFRELRAALP
                     AWDGTSPPPDPLFRGLAAGLARARNLALREHVAAALAGEPALRALIDAALAAPRATGQ
                     DERAWLLALDPRWRDGATWRDLGRAVAVPDEHTEVVYVYSGLRRPETLQYRELRRADF
                     EARFGPGLPDRALEPGVLRAIFRPDVN"
     gene            2678..3778
                     /locus_tag="VNU03_11475"
     CDS             2678..3778
                     /locus_tag="VNU03_11475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75128.1"
                     /translation="MDFGLFFLMQRDEQWSEQAVYHSGLEQMLAAEALGYHSVWIAEH
                     HFNDYGLCPAPPVLASFVAARTTTLRLGMGVSLLPLHHPVDLAESLAVLDVVSGGRLD
                     VGIGRGGTLQDYQTFQSDRDDSRARVEEGVALMRRSWSGAPFDFPGRFHTAEQLHVRP
                     QPVQRPHPPLFIASNSEESVLSAAGLGLPTLSSFFVPVPELQRRHKLYREASLAAGRS
                     LREIEALEARSWGMRVVHVAPDHDEALRATEGPFMGYQRRMAVLRSDATGGSVPGSFD
                     RSLLRLRAFREYLDDGWALIGTPAEVTLGLQQYCEATGYERVLLVMALPGLRTDLALR
                     SMRLFADEVAPALVPPDRAAATSPAGTRSSPS"
     gene            complement(3741..4739)
                     /locus_tag="VNU03_11480"
     CDS             complement(3741..4739)
                     /locus_tag="VNU03_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP75129.1"
                     /translation="MSEVQHRVVETNGIRLHVAEQGEGPLVLLCHGFPESWYSWRHQL
                     RALAEAGFHAVAPDMRGYGQSDAPAEIERYTLLHLVGDMVGLLDALGAPTAVIAGHDW
                     GAPVAWHAALLRPDRFRAVIGLSVPFRPRGSMRPTTQMPQTDDAVFYQLYFQDPGVAE
                     AELERDVRTTIRRILYSGSGDRSGSSDGGRAQASLGNPEAVGMVPRRGGFLSHTADVE
                     VLPPWLTEADVDFFACEFSRAGFRGGLNWYRNIDRSWELLAPFAGARVTVPALYVAGD
                     RDLVVAFRGMDQLIPNLVKFVPALRRTIMLPGCGHWTQQERPAEVNSAMIEFLRGL"
     gene            complement(4736..5986)
                     /locus_tag="VNU03_11485"
     CDS             complement(4736..5986)
                     /locus_tag="VNU03_11485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75130.1"
                     /translation="MKDNLRFVDSDMHIMEPPDLFERYLDPRFRDRVILPIGADGRPK
                     RGTIVIDGLPTSMDAEMQQYRKRSRAGGADTHSTQPLSGSRLQSTNRLDFAIERNYDP
                     EAQVMAMAIEGVDIAVLYPTTGLSLIARDNLDPQLSLALCRAYNDWIAEFCRHSPERL
                     KFVVMLPVHDVHLACRELLRGVRELGAVGSFIRPNLVNGHYWHSNYWDPLYSLHEELD
                     VTWGFHEGTGAWNSHMNVLYGENRFYRHVASHWIEMQQALIAMIVGGVFEFHPKLRVG
                     FLEAQNSWVPGLLSRIEWDYPQYRDSHAPYLSLTPREYFRRNCWAAVEGSEPEIEATA
                     GLIGGDRMCVSTDYPHFDSNFPNVSDNVLKHCSRETAAQILMGGAHLYGLKDADFEKA
                     DAAVARAAAGANGGAAPRGVPAGR"
     gene            complement(5986..6486)
                     /locus_tag="VNU03_11490"
     CDS             complement(5986..6486)
                     /locus_tag="VNU03_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75131.1"
                     /translation="MAYTLETFAAAAHDLLTAEPGPTGREKVRSLLESVLKDDAFVAR
                     HLTDDVPERKILYTDAQLGFCILAHNYRDARESKPHDHGPTWAIYGQARGETMMNDWA
                     LVEPASEARPGKVRHVRTYPLKPGMAHVYNEGDLHSPRRDGPTRLIRIEGTNMDKVKR
                     LSYERA"
BASE COUNT          925 a         2428 c         2426 g         1017 t
ORIGIN      
        1 tgctgcacga gctgcacctg cgagctcatc tgcgggaagt actcgtagta gaacagggtc
       61 gtctcgaagc cgttcgggtg gccggcctcg gcgaggagct tcctggcctc cgccgggcgg
      121 tactgccacc acggcccgag ctgggcgagc gtgggcggct tctcctggta gtagatgtag
      181 ggcacgcccc acccggccat gccgtgcccc tcgaacacgg tgtcgacctg cttctggcgg
      241 tcgatcgaca tcgagatcgc gcggcgcacc cgcaggtcgt tgaagggcgg cttgtcctgg
      301 gccagggcga tgccgaacgg ggtcagcgcg ttgttgtagg cgtgcacgac cgcgttggcg
      361 ttcgtccgct tgaccgtgtc cacctcggac gggctcgcga gccacatgat gtcgctctgc
      421 ccggtgcgga aggcggccat gcgggtcgcc gcgtccgggg tcgagaggat cacgtactcg
      481 tccacgtagg ggcggccctt gtcgaagtag tcggggttgc gcgccagcac cagccgcacc
      541 ttgcgggtgt gctccttcag ggtgaagggc ccggtcccga tcatgcgctt cttgagatcg
      601 ccgtcctcct ccaggacttc ccgggagaag aggacggaga tgggctcggc cacgttctgg
      661 gggaagaaca cgttgggcgt cttgaggtgg atccggacgg tgtacttgtc cggggtctcc
      721 atggcctcga tctcctggaa ggtgaaggac tgcgcgccct ccttcgcgta ggcctcgaag
      781 cagtacttca cgtcggcggc cacgaactcg cgcccgttca gcgggggcag gttgtgccac
      841 ttcacgccct ggcgcagctt gaacgtccac acccggaaat ccgggctcgc ctgccacgac
      901 tcggcgaggt cgcccttgag ggtgaggtcg gcgggtccgc tcgtctcgtc ggggaacacg
      961 tagcgaacca ggcggctgcc gacgaggccc gcgaactggt agaggccgac cgactgggtc
     1021 agccgcgggt cgatcaccgg cgggtcccac gcggaggcgc gggtgagcac cccgccgcgc
     1081 ttgggattcg ggctggccgg gatccagtcg gggtaggcga cccgcgcctg ggcgtgggcg
     1141 cgtccgcgcc cgccgaccac gagggctccg gcgccgagac cggccaatcc ggcacgcgtg
     1201 aggaactgac ggcggtcgag ccgtgaagga aacggggcga ggaggtcgtc ggacatcgcg
     1261 gctctccttc tcacgtcggc gtgaaggaaa ggacctcgag cgaactcgaa ccggtgcgtc
     1321 attacaccgg cgggagtgcg gggactatgg cgcagggccg gcggcaaagt caagcggcga
     1381 gccggagcgt atagtagccg gccatgggga aggtgctcgg gctgctcgcg gtgctcgcgc
     1441 tcgtcgccgg gccggccccc gccgagaccc tcgggggggt gctggccgcg cgcagcgtgg
     1501 ggtcgccgcc ctcccggctc gtcctcgacc agcccctcgc aacgtaccag gtcctcgacg
     1561 acgcccgcga cctgatcgtg gtctacctcg aggagcccgc cgcagcggga cgcctccagg
     1621 ccgcgcggtt cgagcgtgcc acgcggcgat ggacggtcgc gcggctcgac tggccggtgg
     1681 ctgcggacgg aagcggggcc aaggcgctcg agccggaatg gtgccggtcc ggcctcggga
     1741 tcgagcggtt cccgggcggc ttcctcgtcc gctcgcacct caatccgtcc gccgagtgca
     1801 cggtggtgct ggccccggat ctctcggtgc gcgccgtcct cgccgggtgg ccggtggccg
     1861 cgctcgcgga cggccgtctc gtctaccagc ggaaccaggt ccacttcgcc gcggtccacc
     1921 cggtggccct cgccctcctc gacgcccgcc ggccgcggga gatcgcgctc tacccgccgc
     1981 ggccgccggg tgagatccgg cgcgcccacg tggaccgcct gcgcagcgtg tacaccgcgg
     2041 cctggtgcaa cgcccacaac catccgtgcg atcccgagcg gttcgacgag caactgagcg
     2101 cccgcgtagt cgccgacgcg ggcggcgacg cgctcgcctt cgtgaccgcg tgggacaaca
     2161 cggtgggctg gagcgaggcg gagcgctggg ggcgcctcga gcccttccgc gagctgcgcg
     2221 ccgcgcttcc cgcgtgggac ggcacgagcc cgccacccga tccgctcttc cgcgggctcg
     2281 ccgccggcct cgcgcgcgcc cgcaacctcg cgctgcgaga gcacgtggct gccgcgctcg
     2341 cgggcgagcc cgccctgcgc gcgctgatcg atgccgccct cgcggccccg cgcgcgaccg
     2401 gccaggacga gcgcgcctgg ctgctcgccc tcgacccgcg ctggcgcgat ggcgccacgt
     2461 ggcgggatct cgggcgcgcg gtggcggtgc ccgacgagca caccgaggtc gtctacgtgt
     2521 actcggggct gcggcgcccc gagaccctgc agtaccgcga gctgcgccgc gccgacttcg
     2581 aagcccgctt cggccccggc ctgcccgacc gggcgctcga gcccggcgtc ctgcgcgcga
     2641 tctttcgccc ggatgtgaac tgaccccagg aggatccatg gacttcggac tgttcttcct
     2701 catgcagcgc gacgagcagt ggagcgagca ggcggtctac cattccggcc tcgagcaaat
     2761 gctggccgcc gaggcgctcg gctaccactc cgtctggatc gccgagcacc acttcaacga
     2821 ctacggcctc tgccccgcgc cgccggtcct ggcctccttc gtggccgcgc gcaccaccac
     2881 cctgcgcctc ggcatggggg tgagcctcct gccgctccac cacccggtcg acctggccga
     2941 gtcgctcgcg gtcctcgacg tggtgagcgg cgggcggctc gacgtgggga tcggccgcgg
     3001 cggcacgctg caggactacc agaccttcca gtccgaccgc gacgacagcc gggcgcgggt
     3061 cgaggagggc gtcgcgctca tgcgccgatc ctggagcggc gcgccgttcg acttcccggg
     3121 ccgcttccac accgccgagc agctccacgt acgcccgcaa ccggtgcagc ggccgcaccc
     3181 gccgctgttc atcgcctcca acagcgagga gagcgtgctg tccgccgcgg gtctcgggct
     3241 gcccacgctc tcgtcgttct tcgtgccggt gccggagctg cagcggcggc acaagctcta
     3301 tcgcgaggcc tcgctcgcgg ccggacgctc gctccgcgag atcgaggcgc tcgaggcccg
     3361 ctcgtgggga atgcgggtgg tgcacgtggc gcccgatcac gacgaggcac tgcgtgccac
     3421 cgagggcccg ttcatgggct accagcggcg catggcggtg ctgcgctcgg acgccaccgg
     3481 gggctcggtg ccgggatcgt tcgaccgctc cctgctccgg ctgcgcgcct tccgcgagta
     3541 cctggacgac ggctgggccc tgatcggcac gcccgccgag gtgaccctcg gcctccagca
     3601 atactgcgag gccaccggct acgagcgcgt gctcctggtg atggccctgc ccgggctgcg
     3661 caccgacctg gcgctccggt cgatgcgtct gttcgcggac gaggtcgcgc ccgcgctggt
     3721 gccgccggat cgcgccgccg ctacaagccc cgcaggaact cgatcatcgc cgagttgacc
     3781 tcggccggcc gctcctgctg ggtccagtgc ccgcagccgg gcagcatgat cgtccggcgg
     3841 agggcgggca cgaacttgac gagattcggg atgagctggt ccatcccgcg gaaggccacc
     3901 accaggtcgc ggtcgcccgc cacgtagagc gcgggaaccg tgacccgcgc gcccgcgaac
     3961 ggggccagca gctcccagct ccgatcgatg ttgcggtacc agttgaggcc gccgcgaaag
     4021 cccgcgcggg agaactcgca cgcgaagaag tcgacgtccg cctcggtcag ccacggaggc
     4081 aggacctcca cgtcggcggt gtgactcagg aaccccccgc ggcgcggcac catgcccacc
     4141 gcctcgggat tgcccaggct cgcctgcgcg cgccccccgt cgctagatcc cgagcggtcg
     4201 cccgatcccg agtagaggat gcgccggatg gtggtccgca cgtcccgctc gagctccgcc
     4261 tccgccacgc ccggatcctg gaagtagagc tggtagaaga cagcgtcatc ggtctgcggc
     4321 atctgggtgg tggggcgcat cgagccgcgc ggccggaacg gcacgctcag cccgatcacc
     4381 gcgcggaacc ggtcggggcg cagcagcgcc gcgtgccacg ccaccggcgc gccccagtcg
     4441 tggccggcaa tcaccgcggt cggcgcgccg agcgcgtcga gcaaccccac catgtcgccc
     4501 accaggtgca agagcgtata gcgctcgatc tcggcgggcg catcgctctg gccgtatccg
     4561 cgcatgtccg gcgccaccgc gtggaagccg gcctcggcca gcgcccggag ctggtgccgc
     4621 caggagtacc acgactccgg gaacccgtga cagagcagga ccagcggccc ctcgccctgc
     4681 tccgccacgt gcagccggat cccgttcgtc tcgacgaccc ggtgctggac ctcgctcatc
     4741 ggccggccgg aacgccgcgc ggggcggctc cgccgttcgc gcccgcggcc gcccgcgcca
     4801 ccgccgcgtc cgccttctcg aaatcggcgt ccttgagccc gtagaggtgc gcgccgccca
     4861 tcaggatctg cgccgcggtc tcgcgcgaac agtgcttgag cacgttgtcc gacacgttcg
     4921 ggaagttcga gtcgaagtgc gggtagtcgg tggagacgca catccggtcg cccccgatca
     4981 ggcccgcggt ggcctcgatc tccggctcgc tcccctccac cgcggcccag cagttgcggc
     5041 ggaagtactc gcgcggggtc agggacagat agggcgcgtg ggagtcgcgg tactgcgggt
     5101 agtcccactc gatgcgcgag agcagtcccg gcacccacga gttctgcgcc tccaggaagc
     5161 ccacccgcag cttcgggtgg aactcgaaca ccccgcccac gatcatcgcg atcagggcct
     5221 gctgcatctc gatccagtgg ctcgccacgt ggcggtagaa gcggttctcg ccgtagagca
     5281 cgttcatgtg cgagttccac gccccggtgc cctcgtggaa gccccacgtc acgtcgagct
     5341 cctcgtgcag gctgtagagt gggtcccagt agttcgagtg ccagtagtgg ccgttcacca
     5401 ggttgggccg gatgaacgag ccgaccgcgc ccagctcccg caccccgcgc agcaactcgc
     5461 ggcaggcgag gtgcacgtcg tgcaccggaa gcatcaccac gaacttgagg cgctccgggc
     5521 tgtggcggca gaactccgcg atccagtcgt tgtaggcccg gcacagggcc agcgacagct
     5581 gcgggtccag gttgtcgcgc gcgatcaggc tgaggccggt ggtcgggtag agcaccgcga
     5641 tgtcgacccc ctcgatggcc atcgccatca cctgcgcctc ggggtcgtag ttgcgctcga
     5701 tcgcgaagtc gagccggttg gtggattgca gccgcgagcc ggagagcggc tgggtgctgt
     5761 gggtgtcggc cccacccgcg cggctgcgct tgcggtactg ctgcatctcc gcgtccatcg
     5821 aggtgggcag cccgtcgatg acgatggtgc cgcgcttcgg gcgcccgtcc gcgccgatcg
     5881 gcagaatcac ccggtcgcgg aaccgcgggt cgagataccg ctcgaacaga tcgggcggct
     5941 ccatgatgtg catgtccgag tcgacgaagc ggagattgtc tttcatcagg ccctctcgta
     6001 ggacaggcgc ttgaccttgt ccatgttggt cccctcgatg cggatgagcc gcgtggggcc
     6061 gtcgcgccgt ggggagtgga gatcgccctc gttgtagacg tgggccatgc cgggcttgag
     6121 cgggtaggtc cggacgtggc gcaccttccc gggccgcgcc tcgctcgcgg gctcgacgag
     6181 ggcccagtcg ttcatcatgg tctcgccccg ggcctggccg tagatggccc aggtcggccc
     6241 gtggtcgtgc ggcttgctct cgcgcgcgtc ccggtagttg tgggcgagga tgcagaagcc
     6301 gagctgcgcg tcggtgtaga ggatcttgcg ctccggcacg tcgtcggtca ggtgccgggc
     6361 cacgaaggcg tcgtccttca gaaccgactc cagcaggctc cgcaccttct cgcggccggt
     6421 cggcccgggc tcggcggtga ggagatcgtg ggcggcggcg gcgaaggtct cgagcgtgta
     6481 ggccatcggc agtctcctca atcgtccagc agggtgcgaa acagccgggc caggtccgcc
     6541 ttctcctcga agccgatgcc ggaggccgag cctgttcagg cgcaccctac gccgcccgcc
     6601 aaatggtgtc aatgtaggcc gtcgcgcgat gccgtatcat gccgccggag gagaacgcgc
     6661 acatgggcga gaccgtcgcg tgcatcggca tgggcctgct gggatcggcc ctggccgaga
     6721 acctgatccg ggccggcttc acggtgcgcg gctacgacat cgcgcccgag cgggtacgcg
     6781 aacacgtcgc gcgggg
//
LOCUS       DATMCV010000498         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_963479, whole genome shotgun sequence.
ACCESSION   DATMCV010000498 DATMCV010000000
VERSION     DATMCV010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963479"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VNU03_11495"
     CDS             <1..144
                     /locus_tag="VNU03_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWP75132.1"
                     /translation="AIVLFHIEEMSYRDIAAALEVPMGTVMTWLHRGRAQLKRALEGE
                     SGT"
     gene            249..536
                     /locus_tag="VNU03_11500"
     CDS             249..536
                     /locus_tag="VNU03_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75133.1"
                     /translation="MAIEARDRRQVLTGLVAALVGLAVVAILLGPTLEPAALLASLAK
                     VAADLARWTTGVGVVLALVPLAIWASAALGSIAAVLSLVFVARARSLGLVK"
     gene            579..1757
                     /locus_tag="VNU03_11505"
     CDS             579..1757
                     /locus_tag="VNU03_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75134.1"
                     /translation="MRATKLIVVLAVALMSLPGLAWAATPDTRRLEGRLPDDSPVVSL
                     AQPRGTVLDALSTITKQTGWVLVVTAPESATTRPLTIQVSKRPAGEVLDLVLEAGSLR
                     ASFADGVLRVRSDDTARSADSWRERRRERRGRHGSERVVFGRSLTIGADETVDKAVAI
                     GGSVTVAGHVRYDAVAIGGSVTILPGASVGGDSVAIGGAVSVAEGATLEGDNVSLGGA
                     IPTTVGSMTRWVVGGPHMFSMFRFASRLTRAVLLLVIAVLIAAAFPGALTRIGAYLVT
                     RPGLSALGGVAILLGFVPFCVLLAVTIIGIPLIPVAVLLLVALFLFGFTVSAAWLGER
                     LPFLQEKTPVKTVALGGAVFALVGLIPWIGTVALILAAAFSAGATLLSRFGRTPAVAV
                     "
     gene            1757..>2170
                     /locus_tag="VNU03_11510"
     CDS             1757..>2170
                     /locus_tag="VNU03_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75135.1"
                     /translation="MRRVASWALGVVAAAVAVGTALVYGQVRDAGPIEQVTLRSGSYA
                     DFRQVFVREAPADVSVPATLRFPDQPRDRYPAVVVVHTISGFREANEGWLAAELRRSG
                     FATLTYDSFSARGLSQSAVVGSRAGPPIASGVADAF"
BASE COUNT          252 a          768 c          773 g          377 t
ORIGIN      
        1 gccatcgtcc tcttccacat cgaggagatg tcgtaccggg acatcgccgc ggccctcgag
       61 gtaccgatgg gcacggtgat gacgtggctc catcgcggcc gcgcccagct caagcgggcg
      121 ctggaaggcg aatccgggac atgagcgagc gccccgacga gaacgggcag cttaccagcc
      181 tgctccaggc gcttcgcggg ccggagccat cggccgagtt tctcgccggc gcgcggaggc
      241 gctatctggt ggcgatcgag gcgcgcgacc gacgtcaggt cctcacggga ctcgtggccg
      301 cgctcgtcgg cctggcggtg gtcgcgattc tgcttggccc gaccctcgag ccggccgctc
      361 ttctcgcgtc gctcgcgaaa gtagcggccg acctcgccag atggacgacc ggtgtcggcg
      421 tcgtcctcgc tctcgtgccg ctcgccatct gggcctcggc ggcgctcggc tccatcgcgg
      481 ccgttctgtc cctggtcttc gtcgcgcggg cgcgatcgct cgggctcgtg aaataagcgg
      541 cccgctcgac cgtactccca ggcgaaggga gatcgcacat gcgcgcaacc aagctgatcg
      601 tcgttctcgc cgtcgctctc atgagtctgc ccggcctcgc ctgggcggcc acgccggaca
      661 cccgccggct ggagggccga cttcccgacg actctcccgt cgtctcccta gcccagccgc
      721 gagggacggt cctcgacgcg ctgagcacga tcaccaagca gacgggctgg gttctggtgg
      781 tcaccgcgcc ggagagcgcg accacccgcc cgctcaccat ccaggtctcg aagcgccccg
      841 ccggcgaggt gctcgatctg gttctcgagg ccggctcgct ccgggcctcg ttcgccgacg
      901 gcgtcctccg cgttcgctcg gatgacaccg cgaggtccgc ggattcatgg cgcgagcgac
      961 ggcgggagcg gcgcggtcgc cacgggtccg agcgcgtggt cttcggccga tccctcacga
     1021 tcggcgccga cgagaccgtc gacaaggccg tggccatcgg cggctccgtc acggtcgccg
     1081 gtcacgtgcg atacgatgcg gtggcgatag gcggctcggt cacgatcctg ccaggcgcca
     1141 gtgtcggcgg cgactcggtc gcgatcggcg gggcggtttc ggtcgcggag ggcgccacgc
     1201 tcgagggcga caacgtgagc ctcggcgggg cgatcccgac gacggtcggc tcgatgaccc
     1261 gctgggtggt cggcggacct cacatgttct cgatgttcag gttcgcctca aggctgacgc
     1321 gcgcggtgct gctgctggtc atcgcggtgc tcatcgcggc ggccttcccc ggcgccttga
     1381 cgcgcatcgg ggcgtatctc gtcactcggc ccggtctctc ggcgctcggc ggcgtcgcga
     1441 tcctcctggg attcgtgccc ttctgcgtcc tgctggccgt gacgatcatc ggcatcccgc
     1501 tgatcccggt ggcggtgctg cttctcgtcg ccctcttcct gttcggtttc acggtctcgg
     1561 ccgcatggct cggcgagagg ctgccgttcc ttcaagagaa gacccccgtc aagacggtcg
     1621 cactgggagg cgccgtcttc gccctcgtgg gcctgattcc gtggatcggc accgtcgctc
     1681 tgatcctcgc ggccgccttc tcggccggcg cgacgctcct ctcgcgcttc gggcgcacac
     1741 cagcggtggc ggtctaatgc gacgggtggc gtcgtgggcg ctgggggtgg tggccgccgc
     1801 ggtcgcggtc gggacggcgc ttgtatacgg tcaggtccgc gatgcgggcc cgatcgagca
     1861 ggtgacgctc cgctcgggct cctatgccga cttccgccag gtattcgtgc gggaggcgcc
     1921 ggcggatgta tcggtccccg cgacgctccg ctttcccgat cagccgcggg atcggtatcc
     1981 ggcggtggtg gtggtccaca cgatcagcgg cttccgggag gccaacgagg gctggctggc
     2041 ggcggagctg cgccggagcg ggttcgcgac gctgacctac gacagtttca gcgcgcgggg
     2101 cctgagccag tccgcggtcg tcggctcccg cgccggcccg ccgatcgcgt ccggagtcgc
     2161 cgacgcgttt
//
LOCUS       DATMCV010000499         5419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_493452, whole genome shotgun sequence.
ACCESSION   DATMCV010000499 DATMCV010000000
VERSION     DATMCV010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493452"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VNU03_11515"
     CDS             complement(<1..1290)
                     /locus_tag="VNU03_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWP75136.1"
                     /translation="MRAPAGKTRRRPVMVVVALVAAAFAWLATVPPRAEAATVRHVDR
                     SDSTCGGRAPCYGSIQAAVNAAQPGDTVQVRAGSYVEQVNITGKNASAVSEASRIVIQ
                     ADPTVPAGSVVLHGAVAQCAQGHAIRLQQSRFVTIRGLTITGAGGAGIALLGGSSSNV
                     AIRIERNRIVGNGAAGCDGGSGISIAGGNAGTLVLNNVIVANGRNGIATTDPDGGPHT
                     IVQNTIHGNGWNGLSATRAHVLLLANNAITGNGTQAGSTGGRVGVRRETGSALPAATI
                     VLRNNLVCGNRLGEMAGPVLDAIDGGNLTPTGAEGPGVATSPGCELPATVYRNLAGAD
                     HTPGSLDDDPTPAAGSPLIDRGLDPRTLLTPDLNTRFEADYFEEGVRPVAGAVGSPSR
                     FDVGAVEARRDAEPPALVFIAPPANAHLRGVVSVRAQA"
     gene            1518..2390
                     /locus_tag="VNU03_11520"
     CDS             1518..2390
                     /locus_tag="VNU03_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWP75137.1"
                     /translation="MRLPRDRFDYSPFPARPPWKLPKGARIAVWTIVNIEEWDIEKAM
                     ARQYLPTPQGVAVMPDVPNWAWHDYGMRVGFWRLLDALNKRKIRATTAINAHVVESYE
                     PVARAMLDAGWEFMGHGVVQGAMHLLPDQRKAIRQAVELLEKFTGKKPKGWLGPGLTE
                     TWETLDYLRAEGIEYVSDWVNDDQPYEIRTQSGPLVSVPYSIELNDIPMMVVQHHTSA
                     EWLQRAKDQFDRLYAEGAKNPRVMALAVHPYISGVPHRIKYFEAVYDYMRKKPGVWMT
                     TGEEIYEWYRQSRR"
     gene            2366..3499
                     /locus_tag="VNU03_11525"
     CDS             2366..3499
                     /locus_tag="VNU03_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWP75138.1"
                     /translation="MVQAVASVIVSRRASALLALGLLAAALPGVAHAQGGPKLKVPPG
                     FAIDVFADKVGSVRFMAIDPAGTLLVSEPSAGRVLALPDKNGRGKADSVQTVVTGLDQ
                     PHGLAFHEGALYVAETSRVQRFAYDPATMKATQPTLLTRLPAGGGHWTRTVVFGPDGL
                     MYVSVGSSCNVCRESDKRRAAVLRFNADGSGEQLFASGLRNAVGLAFHPTTGVLWATV
                     NERDWRGDDVPPDYVTELREGAVHGWPDCMTVRGRVIADTSFAKGTTCDKVTLPTVEI
                     QAHSAPIGLAFYTGTQFPEEYRGNLFVAYRGSWNRSLPTGYKIVRIRLRDGQPPAVED
                     FATGWLEGTSAWGRPVDLVVGRDGALYLSDQGAGRIYRISHRP"
     gene            3496..4737
                     /locus_tag="VNU03_11530"
     CDS             3496..4737
                     /locus_tag="VNU03_11530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75139.1"
                     /translation="MTHIDLSPPARAMRAPGANLRWLMLGVIFLTRTSMGFQFQSIAA
                     LTPFVVPAFDLSYAEVGLLMGLFMLPGVVIALPGGLLGQRFGSLRVVIGGLGLLIVGG
                     MIVSYSPGLHTAAFGRSIGGVGGVLVNIMLARMVAGWFRERELQTAMGLMLSAWPFGM
                     ALALVILGPLAAATSWRIAEYVTVVAAGLALALIALAYKEAPAEGAPADGASPEPARF
                     HLGVPGRVWALAVSAGIGWALLNASVIVVASFAPSFLMSRGASVGEAGGITGLALWIS
                     IISVPLGGLVADRLKKPRLLIVVGCVATALTIACAARAPWPALWLVASGILLGLPPGI
                     MMALLPRAVRPEHLATALGVFYALFYLGMAVSQTIAGALRDITGDPAMPLLFAAGLML
                     ATIAAVAVFWRVEGRTTGSVD"
     gene            4743..>5419
                     /locus_tag="VNU03_11535"
     CDS             4743..>5419
                     /locus_tag="VNU03_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HWP75140.1"
                     /translation="MREPGDVLLVACYELGHQPLAVAWPAAFLERAGYRPALMDTSVE
                     PVDAEKLARAKAVAVSVPMHTALRLGLEVAARARAVNPGAHVCFFGLYATLNAEYLFA
                     HGVDSVIGGEAETPLLALIEALERGAAGPLPGVGRPGQPAAPHLRRIDFPVPSRAGLP
                     SIKKYAHLERDGRTELAAYVESSRGCLHRCRHCPIPPVYGGRFFAVPREVVLADVRQQ
                     IEAGARHV"
BASE COUNT          692 a         1993 c         1923 g          811 t
ORIGIN      
        1 agcctgcgcg cgcaccgaca cgacgccgcg cagatgcgca ttggccggcg gcgcgatgaa
       61 caccagggcg ggcggctcgg cgtcgcggcg cgcctccacc gcaccgacgt cgaaccgcga
      121 gggcgagccc acggcaccgg ccaccgggcg cacgccctcc tcgaagtagt cggcctcgaa
      181 ccgtgtgttc aggtccggcg tcagcagcgt gcgcgggtcg agcccacgat cgatgagcgg
      241 ggagccggcg gccggcgtgg gatcgtcgtc cagcgagccc ggcgtgtgat cggccccggc
      301 caggttgcgg tagacggtgg ccggaagctc gcagcccggg ctcgtggcca ccccggggcc
      361 ctcggcgccg gtcggcgtca gattgccgcc gtcaatcgcg tccagcaccg gtccggccat
      421 ctcgcccagc cggttcccac acacgaggtt gttccgcagc acgatcgtgg ccgcggggag
      481 cgccgagcct gtctcccgtc gcaccccgac ccggccgccg gtggatccgg cctgcgtccc
      541 gttgccggtg atggcgttgt tggccagcag cagcacgtgg gctcgcgtgg cagagagtcc
      601 gttccagccg ttgccgtgga tcgtgttctg cacgatcgtg tgcggcccgc cgtcgggatc
      661 ggtcgtggcg atcccgttgc gcccgttcgc gacgatcacg ttgttgagca ccagcgtccc
      721 ggcattgccg cccgcgatgc tgatgccgct gccgccgtcg cacccggccg cgccgttgcc
      781 gacgatgcga ttgcgctcga tccggatcgc gacgttcgag ctgcttccgc ccagcagcgc
      841 gatgcccgcg ccgccggcgc cggtgatcgt gaggccgcgg atcgtcacga agcgcgactg
      901 ctggagccgg atcgcgtggc cctgcgcgca ttgcgcgacc gcgccgtgca gcacgacgct
      961 ccccgccggc acggtgggat ccgcctggat cacgatgcgg ctcgcctccg agaccgcgct
     1021 cgcgttcttg ccggtgatgt tgacctgctc gacgtagcta ccggcccgca cctgcacggt
     1081 gtcgcccggc tgcgccgcgt tcaccgcggc ctggatgctg ccgtagcagg gagcgcgccc
     1141 gccgcaggtg gaatccgagc ggtccacgtg ccgcacagtg gccgcctcgg cgcgcggcgg
     1201 aacggtcgcg agccaggcaa acgcggccgc gaccagggcc accacgacca tcaccggacg
     1261 acgccgggtc ttgcccgccg gagcgcgcat ggtgagaggg gagagcagca cacgtgccac
     1321 gcaacgtggc cgagcgtccc gtgaggcttc tcgcgctctt gggagaccgg acccgtcagt
     1381 ctccagcgat cgcttcacag gccacggtgc acgtcacctc cggggctgcc gccccggctg
     1441 ggcaagcgcc ctcgggggtc ttgtcccccg tggcgcttcc cgtatagtcg agtagactcc
     1501 gcgagggagg aggccccgtg agactgcccc gcgaccgctt cgactactcg cccttccccg
     1561 cccgcccacc ctggaagctc cccaagggcg cccgcatcgc agtgtggacc atcgtcaaca
     1621 tcgaggagtg ggacatcgag aaggcaatgg cgcgccagta cctgcccacg ccccagggcg
     1681 tggccgtcat gcccgacgtg cccaactggg cgtggcacga ctacgggatg cgggtggggt
     1741 tctggcggct gctggacgcg ctcaacaagc ggaagatccg cgcgaccacc gcgatcaacg
     1801 cccacgtggt ggaatcctac gagccggtcg cccgcgcgat gctcgacgcc ggctgggagt
     1861 tcatgggcca cggggtcgtc cagggggcga tgcacctgct ccccgaccag cgcaaggcca
     1921 tccgccaggc cgtcgagctg ctggagaagt tcaccggcaa gaagccgaag ggctggctcg
     1981 ggcccggcct caccgagacg tgggagacgc tcgactatct ccgcgccgag ggcatcgagt
     2041 acgtctccga ctgggtcaac gacgatcagc cctacgagat ccgcacccag tcgggcccgc
     2101 tcgtgtccgt gccctattcg atcgagctga acgacatccc gatgatggtg gtgcagcacc
     2161 acacctcggc cgagtggctg cagcgtgcga aggatcagtt cgaccgcctc tacgccgagg
     2221 gcgcgaagaa cccgcgggtg atggcgctgg ccgtccatcc ttatatctcg ggcgtcccgc
     2281 accgcatcaa gtacttcgag gcggtgtacg actacatgcg caagaagccc ggcgtgtgga
     2341 tgaccaccgg cgaggagatc tacgagtggt acaggcagtc gcgtcggtga tcgtgtcgcg
     2401 ccgggcctcc gccctgctgg ccctgggcct gctggccgcc gccctgcccg gcgtggcgca
     2461 cgcccagggt gggccgaagc tcaaggtgcc gcccggcttc gccatcgacg tgttcgcgga
     2521 caaggtcggc agcgtgcgct tcatggccat cgaccccgcc ggcacccttc tggtctccga
     2581 gccctccgcg ggccgcgtgc tggccctgcc cgacaagaat ggccgcggca aggccgattc
     2641 cgtgcagacg gtggtgaccg gcctcgatca gccgcacggc ctggccttcc acgagggcgc
     2701 gctctacgtc gcggagacct cgcgcgtgca gcgcttcgcc tacgacccgg ccaccatgaa
     2761 ggccacgcag cccaccctgc tgacacgcct gcccgcgggc ggcggccact ggacgcgcac
     2821 cgtggtcttc gggcccgacg ggctcatgta cgtctcggtg ggctcgtcct gcaacgtctg
     2881 ccgcgagagc gacaagcgcc gcgccgcggt gctgcgcttc aacgcggacg gctcgggcga
     2941 gcagctcttc gcctccggcc tccgcaacgc ggtgggcctg gccttccacc cgaccaccgg
     3001 cgtgctctgg gccaccgtga acgagcgcga ctggcgcggc gacgacgtgc cgcccgacta
     3061 cgtgaccgag ctccgcgagg gcgccgtgca cggctggccc gactgcatga cggtgcgcgg
     3121 ccgcgtgatc gccgacacca gcttcgcgaa gggcaccacg tgcgacaagg tgacgctgcc
     3181 gaccgtggag atccaggcgc actcggcgcc gatcgggctc gccttctaca ccggcaccca
     3241 gttcccggag gagtaccggg gcaacctctt cgtggcctat cgcggctcgt ggaaccgcag
     3301 cctgcccacc ggctacaaga tcgtgcgcat ccgtctgcgc gacggccagc cgccggcggt
     3361 ggaggacttc gccaccggct ggctcgaggg cacctcggcg tggggccggc ccgtcgacct
     3421 ggtggtgggg cgcgacggcg ctctctatct ctcggaccag ggcgccggcc gcatctaccg
     3481 catcagccat cggccatgac ccacatcgat ctgtctccac ccgcgcgcgc gatgcgcgcg
     3541 cccggcgcga accttcgctg gctcatgctg ggcgtcatct tcctcacgcg cacctcgatg
     3601 ggcttccagt tccagtcgat cgccgcgctc accccgttcg tggtgcccgc cttcgacctg
     3661 agctacgccg aggtgggcct gctgatgggc ctcttcatgc tgccgggcgt ggtcatcgcg
     3721 ctgccgggcg gcctgctcgg ccagcgcttc ggcagcctgc gcgtggtgat cggggggctc
     3781 ggcctcctga tcgtgggcgg catgatcgtg tcctacagcc ccgggctcca caccgccgcc
     3841 ttcggccgct cgatcggggg cgtgggcggc gtgctggtga acatcatgct ggcgcgcatg
     3901 gtggccggct ggttccgaga gcgcgagctg cagaccgcga tgggcctgat gctctcggcg
     3961 tggcccttcg gcatggcgct ggcgctcgtc atcctgggcc cgctggccgc ggccacctcg
     4021 tggcgcatcg cggagtacgt gacggtggtc gcggcgggcc tggcgctggc cctgatcgcg
     4081 ctcgcctaca aggaggcccc ggcggagggc gcacccgccg acggcgcgag ccccgagccg
     4141 gcgaggttcc acctgggcgt gccgggccgc gtgtgggccc tggcggtcag cgccggcatc
     4201 gggtgggcgc tgctgaacgc cagcgtcatc gtggtggcga gcttcgcgcc cagcttcctg
     4261 atgtcgcgcg gggcctcggt gggcgaggcg ggcggcatca ccgggctcgc gctctggatc
     4321 agcatcatct cggtgccgct cggcggcctg gtggcggacc gactcaagaa accgcgcctg
     4381 ctgatcgtgg tgggctgcgt ggccaccgcg ctcaccatcg cgtgcgcggc gcgcgcgccc
     4441 tggcccgcgc tctggctggt ggcgagcggc atcctgctgg gcctgccgcc cggcatcatg
     4501 atggcgctgc tgccgcgcgc ggtgcggccc gagcacctgg ccaccgcgct cggcgtgttc
     4561 tacgcgctct tctacctggg catggcagtc tcgcagacca tcgccggcgc cctgcgggac
     4621 atcacgggtg acccggccat gcccttactc ttcgccgcgg ggctcatgct cgccaccatc
     4681 gcggccgtcg ccgtgttctg gcgcgtcgag gggcgcacga cgggcagtgt agactgagcg
     4741 gtatgcgcga gccgggcgac gtcctcctcg tggcctgcta cgagctcggc caccagccgc
     4801 tggcggtggc gtggcccgcc gccttcctcg agcgtgccgg ctaccggccc gcgctgatgg
     4861 acacctcggt cgagccggtg gacgcggaga agctggcgcg cgccaaggcg gtggcggtct
     4921 cggtgcccat gcacaccgcg ctgcgcctcg ggctcgaggt cgccgcgcgc gcccgcgcgg
     4981 tcaatcccgg cgcccacgtc tgcttcttcg ggctctacgc cacgctcaac gcggagtacc
     5041 tcttcgccca cggcgtcgac agcgtcatcg ggggcgaggc ggagacgccg ctgctggctc
     5101 tgatcgaggc gctcgagcgc ggcgccgcgg gcccgttgcc cggggttggt cggccgggcc
     5161 agccggccgc gccgcatctc cggcgcatcg acttcccggt gccgagccgc gcggggctgc
     5221 cgtcgatcaa gaagtacgcg cacctcgagc gcgacggccg cacggagctg gccgcctacg
     5281 tggaatcgag ccgcggctgc ctgcaccgct gccgccactg cccgatcccg ccggtgtacg
     5341 gtgggcgctt cttcgcggtg ccgcgcgagg tcgtgctggc cgacgtgcgt cagcagatcg
     5401 aggcgggcgc gcgccacgt
//
LOCUS       DATMCV010000500         3053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_293989, whole genome shotgun sequence.
ACCESSION   DATMCV010000500 DATMCV010000000
VERSION     DATMCV010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293989"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VNU03_11540"
     CDS             <1..247
                     /locus_tag="VNU03_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75141.1"
                     /translation="LTLSVTGLAFAQQPQAQPAPALPQHTERGTGTGMTNPATTEGKP
                     ITTYGQVTVERGTGTGMTNPATTDGKPITTSTSFGQR"
     gene            339..572
                     /locus_tag="VNU03_11545"
     CDS             339..572
                     /locus_tag="VNU03_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75142.1"
                     /translation="MDGLRQIAVVAQGDGWNLLGLDAEGRVWYGSPRRTTKGRSLTWA
                     LMDESAEEDPEPESATLTPTEGTGRAWPSRPRP"
     gene            complement(582..1901)
                     /locus_tag="VNU03_11550"
     CDS             complement(582..1901)
                     /locus_tag="VNU03_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWP75143.1"
                     /translation="MTPDIPPPRPAPGGLPGPEPVARPWRARRFFAELGPGLITGAAD
                     DDPSGISTYSVAGATFGYAPLWTALLSFPLMAAVQLMCARLGMVSGRGLASVIRLHYS
                     RRVLWGACALLVVANVINIGADLGGMAEVSEMVTGVKAVVWTPLYAGFIVSLLFWSSY
                     RQIARVFKWLTLVLFAYVVAAFLARPDWGAVLRATFVPHIEWSNAYLAVFVGILGTTI
                     SPYLFFWQAAQEVEEDRAAGRRTVRQRRGASDADLKRMRTDVLTGMFFSNLVMYFIIL
                     TTAATLHAHGETRIETARQAAEALRPLAGAGAYWLFALGLIGTGMLGVPVLAGSCAYA
                     VAEAEGWRGSLDDTPRLARRFYAVVAVAMLVGLILNYVGLDAVTMLFWSAVVNGVLAA
                     PLIVLVVLLTSDRAVMGKRVNPPLLRWLGWATAAIMTAATVGMLVTL"
     gene            1956..>3053
                     /locus_tag="VNU03_11555"
     CDS             1956..>3053
                     /locus_tag="VNU03_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HWP75144.1"
                     /translation="MDRSGVSNRDQLRAIAHRAMAERGLLPDFSPAAQEETEAIRQAP
                     TDASPEIRDLRDLLWCSIDNDDSRDLDQLTVTASMREGREGTVKVLVAVADVDALVKP
                     GSAIDGHARANTTSVYTAAEIFPMLPEKLSTDLTSLGHDETRLATVVEMTVGRDGTVT
                     ASEIYRALVRNRAKLAYNGVAAWLDGTAPAPPRLAAVPGLDEQLRIQDRVAQAMKALR
                     HQRGALSLETRESRAVFDGDLLADLRPDEKNRAKELIEDFMIAANGVTARYLEAKGLP
                     SLRRVLRAPERWPRLVELAAAQGGRLPPEPDARALEDFLARRHQADPERFPDLSLSVV
                     KLLGRGEYAVERPGQSPDGHFGLATRDYSHST"
BASE COUNT          523 a         1102 c         1061 g          367 t
ORIGIN      
        1 actcaccctc tcggtcaccg ggcttgcctt cgcgcagcag ccacaagcgc aacccgcccc
       61 cgcactcccg cagcatacgg agcgcggaac gggcaccggc atgaccaacc ccgccacgac
      121 ggaaggcaaa ccgatcacga cctacggtca ggtcacggtc gagcgcggaa cgggcacggg
      181 catgaccaac cccgccacga cggacggcaa gccgatcacg acctcgacga gcttcggtca
      241 gcggtagcag ccggcccaca gcctcaccgg ccaacatcca cgagcgccga gtccctcggc
      301 gctcgtgctg tgtccggacc agacgaagga gatcaaccat ggacggtctc agacagatcg
      361 cggtggtcgc acagggcgat gggtggaact tgctggggct cgatgcggag ggccgggtct
      421 ggtacggctc gccgcgacgg acaacgaagg ggcggtcgct cacctgggcc ctgatggacg
      481 agagcgcgga agaggacccg gagccggagt ccgccaccct gacgcccacg gaagggaccg
      541 gccgagcgtg gccctcgcgt ccgcgcccct gaggcgctcc ctcacagcgt cacgagcatt
      601 cccaccgtcg ccgcggtcat gatggccgcg gtggcccacc cgagccaccg caggagcggg
      661 gggttcaccc gcttgcccat gaccgcgcga tcgctggtga gcagcaccac cagcacgatc
      721 agcggtgccg cgagcactcc gttgacgacg gccgaccaga acagcatggt gaccgcgtcg
      781 agccccacgt agttcaggat caggccgacc agcatcgcga ccgccaccac cgcgtagaag
      841 cgccgcgcga ggcggggggt gtcgtcgagg gagccccgcc agccctccgc ctcggcaacc
      901 gcgtaagcgc acgacccggc caggaccggc actccgagca tcccggtgcc gatcagcccg
      961 agggcgaaga gccagtaggc gcctgctccc gccagcggcc ggagggcctc ggcggcctgg
     1021 cgcgccgtct cgatgcgcgt ctccccgtgc gcgtgcagcg tcgcggcggt ggtgaggatg
     1081 atgaagtaca tgacgaggtt cgagaagaac atgccggtca gcacgtccgt ccgcatccgc
     1141 ttcaggtccg cgtcgctcgc gccgcggcgc tgccgcacgg tgcgccggcc ggccgcgcga
     1201 tcctcctcga cctcctgggc ggcctgccag aagaagagat agggcgagat cgtggtgccc
     1261 aggatgccga cgaagacggc gaggtaggcg ttggaccact cgatgtgcgg gacgaaggtg
     1321 gccctcagca ccgcccccca gtcggggcgg gcgaggaacg cggccaccac gtaggcgaac
     1381 aggacgagag tgagccactt gaagacgcgc gcgatctggc gataggacga ccagaagagc
     1441 agggacacga tgaagccggc gtagagcggc gtccagacca cggccttcac gccggtcacc
     1501 atctcgctca cctcggccat gcctccgaga tccgcgccga tgttgatgac gttcgccacc
     1561 accagcagag cgcacgcgcc ccagagcacg cgccgggagt agtggagccg gatgacgctc
     1621 gccaggcccc gccccgacac catgccgagg cgcgcgcaca tcagctggac cgcggccatc
     1681 aggggaaacg ataagagcgc ggtccagagc ggagcgtacc cgaaggtcgc gcccgcgacc
     1741 gagtaggtgg agatgcccga cgggtcgtcg tcggccgctc ccgtgatgag acccggtccg
     1801 agctcggcga agaaccgtcg cgcccgccac ggccgtgcga ccggctccgg gcccgggagt
     1861 cctccgggag cgggacgcgg cggcgggatg tcgggagtca tcgggggaga gtcatcgcgg
     1921 acgcccccga gtctacctca gctagaatga gcggcatgga tcgatccggc gtctcgaatc
     1981 gcgatcagct ccgagccatc gcgcaccggg cgatggccga gcgtggactg ctgccggatt
     2041 tctcccccgc ggcgcaggag gagaccgagg cgatccggca ggccccgacc gacgcgagcc
     2101 ccgagatccg agaccttcgc gatctcttgt ggtgctcgat cgacaacgac gactcgcgcg
     2161 acctcgatca gctcaccgtc accgcctcta tgcgcgaggg gcgcgagggg acggtcaagg
     2221 tgctcgtcgc ggtcgccgac gtcgacgcgc tggtcaagcc gggctcggcc atcgacggcc
     2281 acgcgcgggc caacacgacc tcggtgtaca ccgcggccga gatcttcccg atgctccccg
     2341 agaagctctc gaccgatctc acgtccctcg gtcacgacga aacgcgtctg gccaccgtcg
     2401 tcgagatgac ggtcggccgc gacgggacgg ttaccgcctc cgagatctac cgcgccctcg
     2461 tgagaaaccg cgccaagctc gcgtacaacg gcgtcgccgc ctggctcgac gggaccgcgc
     2521 ccgcgccccc gcgcctcgcc gcggtcccgg ggctcgacga gcagctccgg atccaggatc
     2581 gggtcgcgca ggccatgaag gccctccggc atcagcgggg cgcgctgagc ctcgagacgc
     2641 gggagagccg ggccgtgttc gacggcgacc tgctcgccga cctccggccg gacgagaaga
     2701 accgggccaa ggagctgatc gaggacttca tgatcgcggc caacggggtc accgcgagat
     2761 acctggaggc gaagggcctg ccctcgctgc gccgggtgct gcgggccccc gagcgctggc
     2821 cgcgcctcgt cgagctggcc gccgcgcagg gtggccggct cccacccgag ccggacgcac
     2881 gcgcgctcga ggactttctc gcccggcggc accaggcgga tccggagcgg ttcccggatc
     2941 tgtccctctc ggtcgtcaag ctgctgggcc ggggggagta tgccgtcgag cgcccggggc
     3001 aatccccgga cggccacttc gggctggcca cgcgcgacta ctcccattcg acg
//
LOCUS       DATMCV010000501         2445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_761143, whole genome shotgun sequence.
ACCESSION   DATMCV010000501 DATMCV010000000
VERSION     DATMCV010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761143"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VNU03_11560"
     CDS             complement(<1..1061)
                     /locus_tag="VNU03_11560"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP75145.1"
                     /translation="MKRDLGAQERLDAAFAREERRGLMLAAAARSAAVVFILGWLSVA
                     SPERGAAYVWVLGWAAFFLVTGLAQLWLYFRGRALRLAAYLLILVDSLALAASLLLPN
                     PYVSPQVPLAIPLRFATFMYFFIMLMQAAFSFRPRLLLWAGLCGAGAWTAGFLWIFTR
                     PETITDPPSGASRAVMRAAYFDPNYVSMLKYQNEVIVFLLVSAGLALLVRRSRTLVAE
                     RAEAERARGNLSRYFSPKVVDTLAERDEPLGRVRRQAVGVLFADLVGFTTMAEEMTPE
                     EVMTLLRDFHGRMEEEVFRHGGCLEKFIGDALLATFGVPDTGTRDATDALACARGMLA
                     ARSAWNMEREAAGLRPLQIV"
     gene            complement(1099..2136)
                     /locus_tag="VNU03_11565"
     CDS             complement(1099..2136)
                     /locus_tag="VNU03_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP75146.1"
                     /translation="MKLGLSIGYSGAHLDLPVARVQLAERLGYDSVWTAEAYGSDALT
                     PLAYLAAVTRRIRLGTGIMQLAARTPAAAAMAVGTIDALAGGGRVIAGLGVSGPQIVE
                     GWYGQPWGKPYWRLRDYVAIMRKVFARKSPVQHEGREISLPYTGPGSAGLAKPLTSIL
                     HMRPDIPIWLGTGGESTVKLTAEVADGWLPLGFTPARMKAFRPWLDEGLRRAGGGKTL
                     RDLEIQPRAFVVLTDDVRAGLQTLKPRVALYVGGMGHRDKNFHTQAMTANGYGEAADR
                     IQELYLAGRKAEAVAAVPDDFIDEGALVGPKDRIRERFRAWADSGATGLTVATQQDEA
                     IELMADLAGCR"
     gene            complement(2143..>2445)
                     /locus_tag="VNU03_11570"
     CDS             complement(2143..>2445)
                     /locus_tag="VNU03_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75147.1"
                     /translation="HDQTFAEASGNPTVFAMGMLNAGILSRVVTGFAGRPNVRRYKVR
                     FQTRAWPGDDVVCRGTVTRKLEQGGEKLVEGELEAVNQKGETLISGSFVAALPSRG"
BASE COUNT          362 a          878 c          885 g          320 t
ORIGIN      
        1 acgatctgga gaggccgcaa ccccgccgcc tcccgctcca tgttccaggc ggagcgagcc
       61 gccagcatgc cgcgggcgca ggcgagcgcg tcggtcgcgt cccgcgtgcc ggtgtcgggc
      121 acgccgaagg tggccaggag cgcgtcgccg atgaacttct ccaggcagcc gccgtggcgg
      181 aacacttcct cttccatgcg accgtggaag tcccgcagga gcgtcatgac ctcctccggg
      241 gtcatctcct cggccatggt ggtgaagccc accaggtcgg cgaagagcac gccgaccgcc
      301 tgccggcgca cgcggcccag cggctcgtct cgctcggcga gggtgtccac caccttgggt
      361 gagaagtagc gggagaggtt gccgcgggcg cgctcggcct cggcacgctc ggccaccagc
      421 gtgcgcgacc ggcggaccag aagcgcgaga cccgcgctca ccagcaggaa gacgatgacc
      481 tcgttctggt acttgagcat cgagacgtag ttgggatcga agtaggcggc tcgcatcacg
      541 gcgcgactgg ccccgctggg cgggtcggtg atggtctcgg gccgggtgaa gatccagaga
      601 aagccggccg tccaggcgcc ggcgccgcag agtccggccc acaggagcag ccggggccgg
      661 aacgagaacg cggcctgcat gagcatgatg aagaagtaca tgaacgtggc gaagcgcagc
      721 gggattgcga gcggcacctg cggggagacg taggggttcg gcagcaggag ggacgcggcc
      781 agcgccagcg agtcgacgag gatcagcagg tacgcggcca gccgcagcgc gcggccccgg
      841 aagtagagcc agagctgcgc gaggccggtc acgaggaaga aggcggccca gccgaggacc
      901 cagacgtagg ccgcgccgcg ctcggggctc gccaccgaga gccatcccag gatgaacacc
      961 accgccgccg agcgggcggc ggccgccagc atcaggcccc gccgctcctc gcgggcgaag
     1021 gcagcgtcca gccgctcctg ggccccgagg tctcgcttca accttgctcg gtccggggct
     1081 aggtccgagc cggcgccctc atcggcaccc ggccaggtcc gccatcagct cgatggcctc
     1141 gtcctgctgg gtggccacgg tgaggcccgt cgcgccgctg tcggcccacg cgcggaagcg
     1201 ctcgcggatc cggtccttcg gccccaccag cgcgccctcg tcgatgaagt cgtcgggcac
     1261 cgcggccacc gcctccgcct tgcggccggc cagatagagc tcctggatgc ggtcggccgc
     1321 ttcgccatag ccgttcgcgg tcatcgcctg ggtgtggaag ttcttgtcgc ggtggcccat
     1381 gccgcccacg tagagcgcga cgcgcggctt gagggtctgc agcccggcgc gcacgtcgtc
     1441 ggtcagcacc acgaaggcgc gcggctggat ctcgaggtcg cgcagcgtct tgccgccgcc
     1501 cgcgcggcgg agcccctcgt ccagccaggg ccggaaggcc ttcatgcggg cgggcgtgaa
     1561 gcccaggggt agccagccgt ccgcgacctc ggcggtgagc ttcacggtgg actcgccgcc
     1621 cgtccccagc cagatgggaa tgtccgggcg catgtgcagg atcgaggtga ggggcttggc
     1681 cagtccggcc gagccggggc ccgtgtaggg cagcgagatc tcgcggccct cgtgctgcac
     1741 gggcgacttg cgggcgaaga ctttcctcat gatggcgacg tagtcgcgca ggcgccagta
     1801 aggcttgccc cagggctgtc cgtaccagcc ctccacgatc tgcggtcccg acacgccgag
     1861 gcccgcgatg acgcgcccgc cgccggccag cgcatcgatg gtgccgacgg ccatcgcggc
     1921 cgccgcgggg gtacgggcgg cgagctgcat gatcccggtg ccgagccgga tgcgcctggt
     1981 caccgccgcc aggtaggcca gcggcgtcag ggcgtccgag ccgtaggcct ccgccgtcca
     2041 caccgagtcg tagcccagtc gctccgcgag ctgcacgcgc gccaccggca ggtcgagatg
     2101 ggcgccggag tacccgatcg agagcccgag cttcatggcc gctcagccgc gcgacggcag
     2161 cgccgcgacg aaggacccgc tgatgagggt ctcgcccttc tggttgaccg cctcgagctc
     2221 gccctccacc agcttctcgc ccccctgctc gagcttgcgc gtcaccgtgc cccggcacac
     2281 cacgtcatcg cccggccagg cccgcgtctg gaagcgcacc ttgtagcggc gcacgttggg
     2341 ccggcccgcg aacccggtga ccacccggct caggatgccc gcgttgagca tgcccatggc
     2401 gaacacggtg gggttcccgg aggcctccgc gaaggtctgg tcgtg
//
LOCUS       DATMCV010000502         4327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_361969, whole genome shotgun sequence.
ACCESSION   DATMCV010000502 DATMCV010000000
VERSION     DATMCV010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361969"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VNU03_11575"
     CDS             <1..1144
                     /locus_tag="VNU03_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HWP75148.1"
                     /translation="FGSTTAKAVVVSPERELLFTCYALSKGNPIEDAQSLFRQVREAG
                     YGNVGGLALTGYGKDLLRDVVGADVAVVETVAHATGTLHFHPDADVICDVGGTDVKIM
                     ILRQGTVADFRLNSQCSSGNGAFLQGVAERYSIALEDYAEKAFEAKAMPTLAMGCGVF
                     LQSDIVNQQRKGWAAEEIMAALAAVLPVNVWIYAGQLQNLRSVGRKFVLQGGTHRNMA
                     VVKAQVDFIRGKVPEADVVLHPYSGEAGAIGAALCAADWREAESGRTSRFRGFEAIAA
                     LTYTSTTAADTVCKWCPINCTRTFIDVQLPGAKGRPWSKLPLAAGWERVISGNSCPKG
                     LVEDVNELREVKAKLEETKRDYPNVAEMVRKDAFRRSRTGEPAAAG"
     gene            1077..2285
                     /locus_tag="VNU03_11580"
     CDS             1077..2285
                     /locus_tag="VNU03_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HWP75149.1"
                     /translation="MPRWFGRTRSAAPAPESRPPRAELRIGIPRVLNLWSTHQFWVGF
                     FTELGVDPRHLVFSSDTSEEQGRQYGKGRGTVDCCYPVKCIAGHYGELIFGQREKVDV
                     LFSPMIYNLPSFLSGHVARTLACPRVMAAPENIKAGFMKEKNVFAERGIRYLSPFVSL
                     GEPLIVPKQLWEGLRDAIPGLTAEETAGATAAGYRALDDFNTRLRRKGREVLEWCARE
                     NRACLMVLARPYHMDPGIGHEIEVDLQAYGYPVLWAQYFPVDPDLMDWAFGPDVRAGH
                     IKSPFDIRDVWPSSYSSNTNEILWGAKAAARMPWVACVVRLASYECGMDQPTYTPVQQ
                     IVERSGTLFFSFQDLDSTKPAGSVKIRVETIAHYLERYAGDIIEKKKATMEPGCPLLP
                     RAAEAPTGVL"
     gene            2381..>4327
                     /locus_tag="VNU03_11585"
     CDS             2381..>4327
                     /locus_tag="VNU03_11585"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP75150.1"
                     /translation="MRRRRLFWKYTTFFVLLVTAELLVSGITELYFTYQENQAALVAL
                     QREKALAAASQIEGFIKQIEQQMGWVTQPQRAPAGTTLEQRRFDFFRLQRQVPAITEV
                     SYIDAAGREQLRVSRLAMDAIGSQADLSADPRFVEAKARKVYRGPVYFRKESEPYMVI
                     AIAEGGQAGGVTAAEVNLKFIWDVVSQIKIGKAGQAFVVDGQGALIAHPDISLVLQKT
                     DLTRLDQVKAALAGRPQPGEASQEGTIARNLRNHQVLTAHTPIPSLGWTVFVEQPLEE
                     AFEPLRASVKRTVVLLLLGIVLSVGASLMLARTLERPIRALQAGAVRIGEGNLTDRIE
                     VKTGDELEVLADQFNRMAVQLQESYAGLERKVDARTRELQEALEQQTATAEILGVISG
                     SPTDSQPVMDAVAQSAARLCDAVDAQIFRIEGDFLRSVAIHGAIPKYPGQPITRGFVS
                     GRAVLERRTVHVNDLAEETDREFPDARPRQQATGTRTMLATPLLREGVPIGVITIRRL
                     EVRPFSDKQIALLETFADQAVIAIENVRLFQELQARTQDLSQSLEETGALSEVIRAVS
                     SSLDLREVLDTVARRAVALSDADGCGIFELRPGRQAFDVVVSQGLSAEFVDAVLHLSV
                     GIDHGTISQAAETGEPVQIPDVEDA"
BASE COUNT          681 a         1505 c         1518 g          623 t
ORIGIN      
        1 cttcggcagc accaccgcca aggcggtggt ggtctccccc gagcgcgagc tgctcttcac
       61 ctgctacgcg ctgtcgaagg gcaaccccat cgaggacgcc cagtcgctct tccgccaggt
      121 gcgcgaggcg ggctacggca acgtcggcgg cctcgcgctg accggctacg ggaaagatct
      181 cctgcgagac gtggtcggcg ccgacgtggc ggtggtggag acggtggccc acgccaccgg
      241 cacgctccac ttccatcccg acgccgatgt gatctgcgac gtgggcggca ccgacgtgaa
      301 gatcatgatc ctgcggcagg gcacggtggc cgacttccgc ctcaactcgc agtgctcctc
      361 gggcaacgga gcgttcctcc agggggtggc cgagcgctac tcgatcgcgc tcgaggacta
      421 cgcggagaag gccttcgagg ccaaggcgat gcccaccctc gcgatgggct gcggggtgtt
      481 cctccagtcc gacatcgtga accagcagcg caaggggtgg gccgccgagg agatcatggc
      541 cgcgctcgcc gcggtgctgc cggtgaacgt gtggatctac gcgggacagc tacaaaatct
      601 cagatcggtc gggcgcaagt tcgtgctcca gggcggcacc caccgcaaca tggcggtggt
      661 gaaggcccag gtcgacttca tccgcggcaa ggtgccggag gcagacgtgg tcctgcaccc
      721 gtactcgggc gaggcgggcg ccatcggcgc ggccctctgc gcggccgact ggcgcgaggc
      781 cgagagcggg cggacgagcc ggttccgggg cttcgaggcg atcgcggcgc tgacctacac
      841 cagcaccacc gcggccgaca ccgtctgcaa gtggtgcccg atcaactgca cccgcacctt
      901 catcgacgtg cagctgcccg gggccaaggg ccgaccgtgg agcaagctgc ccctggccgc
      961 gggctgggag cgggtgatca gcggcaactc ctgcccgaag gggctggtcg aggacgtgaa
     1021 cgagctgcgc gaggtgaagg cgaagctgga ggagaccaag cgtgactacc ctaacgttgc
     1081 cgagatggtt cggaaggacg cgttccgccg ctcccgcacc ggagagccgg ccgccgcggg
     1141 ctgagctccg gatcgggatc ccgcgcgtcc tcaacctctg gtccacccac cagttctggg
     1201 tgggcttctt caccgagctg ggggtggacc cgcggcatct ggtcttctcc tccgacacct
     1261 cggaggagca ggggcggcag tacggcaagg gccgcggcac cgtcgactgc tgctacccgg
     1321 tgaagtgcat cgccggtcac tacggcgagc tgatcttcgg ccagcgcgag aaagtcgacg
     1381 tcctcttctc gccgatgatc tacaacctgc cctcgttcct ctcgggccac gtggcgcgca
     1441 cgctggcctg cccgcgggtg atggccgcgc cggagaacat caaggccggt ttcatgaagg
     1501 agaagaacgt gtttgccgag cgaggcatcc gatacttgtc gccgttcgtc tcactggggg
     1561 agccgctcat cgtgcccaag cagctctggg agggtctgcg cgacgcgatc cccggcctca
     1621 ccgccgagga gaccgcgggg gcgacggcgg cgggctaccg cgcgctcgac gacttcaaca
     1681 cgcggctgcg ccggaagggg cgcgaggtgc tcgagtggtg cgcgcgcgag aaccgcgcgt
     1741 gcctgatggt gctggcccgg ccgtatcaca tggacccggg gatcggccac gagatcgagg
     1801 tggatctcca ggcctacggc tacccggtgc tctgggccca gtacttcccg gtcgacccgg
     1861 acctgatgga ctgggccttc ggcccggacg tgcgcgccgg ccacatcaag tcgccgttcg
     1921 acatccgcga cgtctggccg tcgtcctaca gcagcaacac caacgagatc ctgtggggcg
     1981 ccaaggcggc cgcgcgcatg ccgtgggtgg cctgcgtggt gcggctcgcc agctacgagt
     2041 gcggcatgga ccagccgacc tacacccccg tccagcagat cgtggagcgc tccgggaccc
     2101 tcttcttctc cttccaggac ctcgactcga ccaagccggc cggctcggtg aagatccggg
     2161 tcgagaccat cgcccactac ctcgagcgat acgcgggcga catcatcgag aagaagaagg
     2221 ccaccatgga gccgggctgc ccgctcctgc cccgggccgc cgaggcgccg accggggttc
     2281 tttgacaggg ggataccccc ccgcctacac tcgcgagggt gacgccgggg acccctgacc
     2341 agcgcctctc caacctcggt agcccggtcc ggggctcgtg atgcgccgcc gccgcctgtt
     2401 ctggaagtac accaccttct tcgtgctgct cgtgaccgcc gagctgctcg tgagcggcat
     2461 caccgagctc tacttcacct accaggagaa ccaggccgcg ctggtggccc tgcagcgcga
     2521 gaaggccctg gccgccgcct cccagatcga gggcttcatc aagcagatcg agcagcagat
     2581 gggctgggtc acccagcccc agcgcgcgcc cgccgggacg acgctcgagc agcggcgctt
     2641 cgacttcttc cgcctccagc gccaggtgcc cgccatcacc gaggtcagct acatcgacgc
     2701 ggccgggcgc gagcagctcc gcgtctcccg gctggccatg gacgcgatcg gcagccaggc
     2761 cgatctctcc gccgacccgc ggttcgtcga ggccaaggcc cggaaggtct accgggggcc
     2821 ggtgtacttc cggaaagagt ccgagcccta catggtcatc gccatcgcgg agggtggcca
     2881 ggccggcggg gtcaccgcgg ccgaggtgaa cctcaagttc atctgggacg tggtctccca
     2941 gatcaagatc ggcaaggcgg gccaggcctt cgtggtggac gggcagggcg ccctcatcgc
     3001 gcatccggac atcagcctgg tgctccagaa gaccgatctc acccggctcg accaggtgaa
     3061 ggccgcgctg gccggccgcc cgcagcccgg cgaggcctcg caggaaggca ccatcgcccg
     3121 gaacctgcgc aaccatcagg tgctgaccgc ccacaccccg attccctcgc tgggctggac
     3181 ggtgttcgtg gagcagccgc tcgaggaggc cttcgagccg ctgcgggcct cggtgaagcg
     3241 gacggtggtg ctgctcctcc tcggcatcgt gctctcggtg ggggcgagcc tgatgctggc
     3301 ccggaccctg gagcggccga tccgggcgct ccaggcgggc gcggtgcgca tcggcgaggg
     3361 caatctcacc gaccgcatcg aggtgaagac gggcgacgag ctggaggtgc tggccgacca
     3421 gttcaaccgc atggcggtcc agctgcagga gtcgtacgcg ggactcgagc gcaaggtcga
     3481 cgcccgcacc cgggagctgc aggaggccct cgagcagcag accgccaccg cggagatcct
     3541 gggcgtcatc agcggctcgc cgaccgactc ccagccggtg atggacgcgg tggcccagag
     3601 cgcggcgcgg ctgtgcgacg cggtcgacgc gcagatcttc cggatcgagg gcgatttcct
     3661 ccggagcgtg gcgatccacg gcgccatccc gaagtatccc gggcagccca tcacccgggg
     3721 cttcgtgagc ggacgggccg tgctcgagcg acgcacggtg cacgtcaacg acctcgccga
     3781 ggagacggat cgggagttcc cggacgcccg gccccgacag caggccaccg gaacgcggac
     3841 gatgctggcc accccgttgc tccgggaagg cgtgccgatc ggggtcatca ccatccgccg
     3901 gctcgaggtg cggccgttct ccgacaagca gatcgcgctg ctggagacgt tcgcggacca
     3961 ggcggtgatc gcgatcgaga acgtgcgcct gttccaggag ctgcaggcgc gcacccagga
     4021 cctctcgcag tccctcgagg agaccggcgc gctcagcgag gtcatccgcg cggtgagctc
     4081 atcgctcgat ctccgcgagg tcctcgacac ggtggcccgg cgggcggtcg cgctctccga
     4141 cgcggacggc tgcgggatct tcgagctgcg ccccggccgg caggccttcg atgtggtggt
     4201 gagccagggg ctctccgccg agttcgtgga cgcggtgctg cacctctcgg tcgggatcga
     4261 ccacgggacg atcagccagg ccgccgagac cggggagccg gtgcagatcc ccgacgtgga
     4321 ggacgcc
//
LOCUS       DATMCV010000503         5811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_429545, whole genome shotgun sequence.
ACCESSION   DATMCV010000503 DATMCV010000000
VERSION     DATMCV010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429545"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VNU03_11590"
     CDS             <1..967
                     /locus_tag="VNU03_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75151.1"
                     /translation="PYKVVSFKPGVEIVMEAFDGYWRKAPSVKRLVMRSMPEETTRAA
                     ALKSGEVDIAYLLTGPTAEAIRKAPGFTLKAPLLSGAFWLELPEQWDPKSPWHDLRVR
                     QAASLAIDRQAVNQAETLGFSRLTGSIVPRIFQYAVHYEPPAYDPARAKKLLAEAGYP
                     NGFDGGDFYPFPPYTSMGEAIQGYLQAVGIRTTTRTMERAAYFTAWREKKLHGVILVI
                     SAAFGNAATRLEPYATKNGIYAYRSLPEVDDLYSRQGRELDPKKREALLAQIQKTLVD
                     KVINVPIYELAFIWGVGPRVEEGGAGLIPGFSYSAPAEDLRLKAR"
     gene            983..3103
                     /locus_tag="VNU03_11595"
     CDS             983..3103
                     /locus_tag="VNU03_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP75152.1"
                     /translation="MAQYSLGIDIGGTFTDLVVYDHDTGARWSRKVLTTHDDPARAVA
                     SGVAGLLDQARVDPAAFSRVVHATTLFTNALIERKGAVTGLITTAGFADTLEIGRERK
                     YELYDLAITKPEPLVPRQLRLEVPERTKADGRIARRLDTRAVTARSAALARAGVEAIA
                     VVFLHAYANPRHEREAARLIARRHPGIAVTTSHEVAPEIREYERASTTVANAYIKPLA
                     QKYLALMAERVSALGIPAPLLLMLSSGGLTHVAEAQRVPVQMLESGPAAGAIAAAFFG
                     QADGNGRVLAFDMGGTTAKLSLVDGGEPLTAYSFEAARQRRFIEGSGLPIRISTIELI
                     EIGAGGGSIAAIDEIGLLKVGPRSAGSHPGPAAYGLGGKEPTVTDADFLLGYLNPDYF
                     AGGEVAVDMAAAIGAVEGLARRLSLESTKVAWGIHDIVNENMAGAARVHIAERGRDPR
                     DYALLCTGGAGPVHAYSVARKLGIGRVICPPSAGVASALGLLVAPARVDRGATVGIRL
                     DRGSIAELEAAFTRLEEQARAVMAETGLKLESATVERLADGRFMGQGFDLVVPLPAGP
                     YEDSDQGRAVLRGAFEGAYREKFALTPPGVPVEFINVRVAVRAPVSGSDVVLEGRRGE
                     GARAALKGTRRAYFPGADGWVETSVYDRYRLGVGDRLSGPAVVEEEGSTLVIGPDATA
                     EVAPSGNIVVTLAPAGGEGRVRGR"
     gene            3100..4722
                     /locus_tag="VNU03_11600"
     CDS             3100..4722
                     /locus_tag="VNU03_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP75153.1"
                     /translation="MTPFDAITLEVLWTRLISVVDEAAKAIVRTSFSTLSNEANDFAC
                     VLTDARGHALAQNSGSIPSFIGTLPATVRHFLRELGPERLKPGDVLITNDPWQGTGHM
                     SDVCLVKPIFFDSGSQRRLVAFSATTAHMPDIGGRIRAIEARELFEEGFHIPLTHLIR
                     EGRVDDTLVALLRANVRTPDQTVGDIWAQVSANELMENRVKRLMEEYALTSLEGLGDE
                     LFGRSERAMREAIRAVPDGTYRYGIDTDGFEAPYRFRVALTVAGDRIVADFTGTSPAQ
                     PRAINCVMAYTYAMTAYAVRCALLPGLPNNEGMYRPVTVEAPEGCLLNPKFPAAVVSR
                     AQTGHYVPILILGALHQVIPDRVAAAAGSPLWALAQSGVRDDGRPYTTVLFFNGGMGA
                     TASKDGEHVLSWPSNISSTPVEVAERNSPLFFHYKRMRPGSGGRGRFRGGLGQDILIE
                     SESARPIVMSFMAERTRFPAPGLAGGGAGGLGDVRINGKKIDHRRQHVLERGDRVLVS
                     TPGGGGYGPAGKRTRELRERDRQRGYTGRGER"
     gene            4733..5422
                     /locus_tag="VNU03_11605"
     CDS             4733..5422
                     /locus_tag="VNU03_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75154.1"
                     /translation="MRGAALTLGLSSLALALFPQFRPFFFFDPTHPMETMDAAAPAVI
                     SGLWRFAHYLALIGFVLLLCALPALRARLAAAGVEARARLATLLCGLGVALILPTLGV
                     ELYALPAIGRIYLAGNVSIAPAVSLIYIGGATLVMLLGLLLLAIGAIVLAVAVARSRA
                     LPRWAGIVYAIGLAAWCPLFPPIVRVVDGLLIGVGGIALAWALRRPSPPPDAPGFHPH
                     ASGSHPVAPQP"
     gene            complement(5456..>5811)
                     /locus_tag="VNU03_11610"
     CDS             complement(5456..>5811)
                     /locus_tag="VNU03_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWP75155.1"
                     /translation="RFAAAYALERGIIIADTKFEFGTDRDGRLVLGDEALTPDSSRFW
                     PADQYAPGGPQPSFDKQYVRDFCEKIGWGKTYPGPELPEDVVAGTRRRYVEAFEQLTA
                     LPFDRYLANPSVVLA"
BASE COUNT          798 a         2054 c         2140 g          819 t
ORIGIN      
        1 accgtacaag gtggtctcgt tcaagcccgg cgtcgagatc gtgatggagg ccttcgacgg
       61 ctactggcgg aaggccccgt cggtgaagcg cctggtcatg cgctcgatgc cggaggagac
      121 gacgcgcgcg gccgcgctca agagcggcga ggtggacatc gcatatctgc tcaccgggcc
      181 gaccgcggag gccatccgca aggcgcccgg cttcaccctc aaggcgccgc tgctctcggg
      241 cgcattctgg ctcgagctgc cggagcagtg ggacccgaag tcgccctggc acgatctgcg
      301 ggtgcgccag gcggcgagcc tcgcgatcga tcgccaggcg gtgaaccagg cggagacgct
      361 tggcttctcg cgcctgaccg gcagcatcgt gccgcgcatc ttccagtacg cggtgcacta
      421 cgagccgccc gcgtacgatc cggcccgggc gaagaagctg ctggccgagg cgggctaccc
      481 caacggcttc gacggcggcg acttctaccc gttcccgccc tacacctcga tgggcgaggc
      541 catccagggc taccttcagg cggtcggcat ccgcaccacg acccgcacca tggagcgggc
      601 cgcctacttc accgcgtggc gcgagaagaa gctccacggg gtgatcctcg tgatcagcgc
      661 cgccttcggc aacgcggcca cccggctcga gccctacgcc acgaagaacg gtatctacgc
      721 gtaccggagc ctgcccgagg tcgacgatct ctactcgcgc cagggtcggg agctcgatcc
      781 caagaagcgc gaggcgctgc tcgcgcagat ccagaagacc ctcgtggaca aggtgatcaa
      841 cgtgccgatc tacgagctgg ccttcatctg gggcgtcggg ccgcgtgtcg aggaaggcgg
      901 ggccgggctc atccccggct tctcctactc ggcgcccgcg gaggaccttc gcctcaaggc
      961 ccgctgagga gagagccccg cggtggccca gtactcgctc gggatcgaca tcggcggcac
     1021 gttcaccgac ctcgtggtct acgaccacga caccggcgcg cgatggagcc gcaaggtcct
     1081 caccacgcat gacgacccgg cgcgcgcggt cgcctccggg gtggcgggcc tgctcgacca
     1141 ggcgcgcgtc gatccggccg cgttctcccg ggtcgtccac gccaccacgc tcttcaccaa
     1201 cgccctgatc gagcgcaagg gcgcggtcac cggcctgatc acgaccgcgg gcttcgcgga
     1261 cacgctcgag atcgggcgcg agcggaagta cgagctgtac gacctcgcga tcaccaagcc
     1321 cgagccgctg gtgccgcggc agctcaggct cgaggtcccc gagcgcacga aggcggacgg
     1381 gcgcatcgcg cggcggctcg acacccgcgc ggtcaccgcg cgctcggccg cgctggcccg
     1441 cgccggggtg gaggccatcg cggtggtgtt cctgcacgcc tacgccaacc cgcgccacga
     1501 gcgcgaggca gcccgactga tcgcgcggcg ccatcccggc atcgcggtga cgacgtcgca
     1561 cgaggtggcc cccgagatcc gcgagtacga gcgggcgtcg accacggtgg cgaacgccta
     1621 catcaagccg ctcgcccaga agtacctggc cctcatggcc gagcgggtgt cggcgctcgg
     1681 gatcccggcg ccgctgctgc tcatgctctc gagcggcggc ctcacccacg tggcggaggc
     1741 gcagcgggtg ccggtgcaga tgctcgagtc cggccccgcc gcgggcgcca tcgcggccgc
     1801 cttcttcggc caggccgacg gcaacgggcg cgtgctcgcc ttcgacatgg gcgggaccac
     1861 cgccaagctc tcgctggtgg acggcgggga gccgctcacc gcgtacagct tcgaggccgc
     1921 gcggcagcgc cgcttcatcg agggcagcgg gctgccgatc cgcatctcga ccatcgagct
     1981 gatcgagatc ggcgcgggcg gcggcagcat cgccgcgatc gacgagatcg ggctcctcaa
     2041 ggtcggccca cggagcgcgg gctcgcaccc ggggccggcg gcctacgggc tcggcggcaa
     2101 ggagcccacc gtgaccgacg ccgacttcct gctgggctat ctcaaccccg actacttcgc
     2161 gggcggcgag gtcgcggtcg acatggcggc ggcgatcggc gcggtcgagg ggctcgcgcg
     2221 gcggctctcg ctcgagtcca ccaaggtcgc gtggggcatc cacgacatcg tcaacgagaa
     2281 catggccggc gcggcgcgcg tgcacatcgc cgagcgcggg cgcgacccgc gcgactacgc
     2341 actgctctgc accggcggcg cggggccagt gcacgcctac tcggtggcgc gcaagctcgg
     2401 gatcggccgt gtgatctgcc cgccgtccgc cggggtggcc tccgcgctcg ggctgctggt
     2461 ggcgccggcg cgggtggacc gcggggccac cgtcggcatc cggctcgatc ggggcagcat
     2521 cgcggagctg gaggccgcgt tcacccgcct cgaggagcag gcgcgcgcgg tgatggcgga
     2581 gaccgggctc aagctcgagt cggccacggt ggagcggctg gccgacgggc gcttcatggg
     2641 ccagggcttc gacctggtgg tgccgctgcc ggcgggaccc tacgaggaca gcgatcaagg
     2701 gcgcgcggtc ctgcgcggcg ccttcgaggg cgcgtatcgc gagaagttcg cgctgacgcc
     2761 gcccggcgtg ccggtggagt tcatcaacgt ccgggtggcg gtgcgcgcgc cggtctcggg
     2821 tagcgacgtg gtgctcgagg ggcgtcgggg cgagggcgcg cgcgccgcgc tcaagggcac
     2881 gcggcgtgcc tacttccccg gcgcggacgg ctgggtggag acctcggtct acgaccgcta
     2941 ccggctcggg gtgggggacc ggctgagcgg cccggcggtg gtggaggaag agggctccac
     3001 gctcgtgatc gggccggacg cgaccgcgga ggtggcgccg agcggcaaca tcgtcgtcac
     3061 cctcgccccc gcagggggag agggcagggt gagggggcga tgacgccgtt cgacgcgatc
     3121 acgctcgagg tgctgtggac ccgcctcatc tcggtggtgg acgaggcggc gaaggcgatc
     3181 gtgcgcacct cgttctccac cctctccaac gaggccaacg acttcgcgtg cgtgctcacc
     3241 gacgcccgcg gccacgcgct ggcccagaac tcgggctcga tcccgtcgtt catcggcacg
     3301 ctgcccgcga cggtgcggca cttcctccgc gagctggggc cggagcgcct gaaaccgggc
     3361 gacgtgctga tcaccaacga cccgtggcag gggaccgggc acatgagcga cgtgtgtctg
     3421 gtgaagccga tcttttttga ctccggcagt caacgccgat tggtcgcctt ctcggcgacc
     3481 acggctcaca tgccggacat cggcgggcgg atccgcgcca tcgaggcccg cgagctgttc
     3541 gaggagggct tccacattcc gctcacgcac ctgattcgcg aggggcgcgt ggacgacacc
     3601 ctggtcgcgc tgctgcgggc caacgtgcgc accccggatc agacggtggg cgacatctgg
     3661 gcccaggtga gcgccaacga gctgatggag aaccgggtga agcggctcat ggaggagtac
     3721 gcgctgacct cgctcgaggg gctgggcgac gagctcttcg gccgctcgga gcgcgcgatg
     3781 cgcgaggcca tccgcgcggt gcccgacggg acctaccgct acggcatcga caccgacggc
     3841 ttcgaggcgc cgtaccgctt ccgcgtcgcg ctcacggtgg cgggcgaccg catcgtggcc
     3901 gacttcaccg gcacgtcacc ggcccagccg cgcgcgatca actgcgtgat ggcctatacc
     3961 tacgccatga ccgcctacgc ggtccggtgc gcgctcctgc ccgggctgcc gaacaacgag
     4021 ggcatgtacc ggccggtgac ggtggaggcg cccgaaggct gcctgctgaa cccgaagttc
     4081 cccgccgcgg tggtgagccg cgcgcagacc ggccactacg tgccgatcct catcctgggc
     4141 gcgctccacc aggtgatccc ggaccgggtg gccgcggcgg cgggctcacc gctctgggcg
     4201 ctggcccagt ccggtgtgcg cgacgacggg cgcccgtaca ccaccgtgct cttcttcaac
     4261 ggaggcatgg gggcgaccgc gtcgaaggac ggtgagcacg tgctctcgtg gccgagcaac
     4321 atctcgagca ccccggtcga ggtggccgag cgcaacagcc cgctcttctt ccactacaag
     4381 cgcatgcggc ccggatcggg cgggcgtggg cgcttccgcg gcgggctcgg ccaggacatc
     4441 ctgatcgaga gcgagtcggc gcgcccgatc gtgatgagct tcatggccga gcgcacgcgc
     4501 ttcccggcgc ccgggctcgc cggcggcggc gcgggcggcc tcggcgacgt gcggatcaac
     4561 ggcaagaaga tcgatcaccg gcgccagcac gtgctcgagc gcggcgaccg cgtgctggtg
     4621 agcacgccgg gcggcggcgg gtacgggccg gcggggaagc gaacgcggga gctgcgggag
     4681 cgcgatcgcc agcgcggcta caccgggcgc ggagagcggt aaggaggcgg ccatgcgtgg
     4741 agcggctctg accctcggcc tgtcgagtct tgccctcgcg ctgttcccgc agttcaggcc
     4801 gttcttcttc ttcgatccga cgcaccccat ggagaccatg gacgcggcgg cccccgcggt
     4861 catctccggc ctgtggcggt tcgcccacta cctggccctg atcggtttcg tgctgctgct
     4921 ctgcgcgctg ccggcgctgc gcgctcgcct tgccgccgcg ggggtcgagg cccgcgcgcg
     4981 actggccacg ctgctctgcg gcctgggggt ggcgctcatc ctgccgacgc tcggggtgga
     5041 gctctacgcg ctgcccgcga tcggacggat ctatctcgcc ggcaacgtgt cgatcgcgcc
     5101 ggcggtgagc ctgatctaca tcggcggcgc caccctggtg atgctgctgg ggctcctcct
     5161 gctcgctatc ggggccatcg tgctggccgt cgcggtggcc cggagccgcg cgctccccag
     5221 gtgggccggc atcgtctacg cgatcgggct cgccgcctgg tgcccgctgt tcccgccgat
     5281 cgtccgcgtg gtggacgggc tcctcatcgg tgtcgggggg atcgcgctgg cgtgggcgct
     5341 gaggcggccc tcgccgcctc cggacgcgcc gggctttcac ccgcacgcgt cgggtagtca
     5401 tcctgtcgcg ccgcagccgt agagcgcccc tcacccgaga gggagagggg aggcatcagg
     5461 ccaggacgac ggaggggttg gccaggtacc tgtcgaaggg cagggcggta agctgctcga
     5521 acgcttcgac gtagcgccgg cgcgtgccgg ccaccacgtc ctcgggtagc tcggggccgg
     5581 ggtaggtctt gccccagccg atcttctcgc agaagtcccg cacgtactgc ttgtcgaagg
     5641 agggctgcgg cccgcccggc gcgtactggt cggccggcca gaagcgcgag gagtcgggcg
     5701 tgagcgcctc gtcgcccagg accagccggc cgtcccggtc ggtgccgaac tcgaacttgg
     5761 tgtcggcgat gatgatgccg cgctcgagcg cgtaggcggc cgcgaatcgg t
//
LOCUS       DATMCV010000504         7695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_823777, whole genome shotgun sequence.
ACCESSION   DATMCV010000504 DATMCV010000000
VERSION     DATMCV010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823777"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..968
                     /locus_tag="VNU03_11615"
     CDS             123..968
                     /locus_tag="VNU03_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWP75156.1"
                     /translation="MLAASLPAAAQTREQIARGKYIFGAAAGCGCHTLPKGGALNAGG
                     RKYDGPFGTVYSSNITPDPANGIGKWTDEQVITATRAGRRPNGERLIPVHPYTVFNGM
                     AEQDLKDVVAYLRSVPAVNKPVPAKKIMPMFESVFLPAWLATFAPRENPPTAAPTTGQ
                     ARGEYLTRAVSHCGECHTPRTMTMATDNSRFLAGAAKGKGPEGSAVPNITPDRETGLG
                     SWTEEQIADYLETGNKPDGDVAGGLMAEVIQGSSTGYKDLTKADRVAIAKYLKSIPPI
                     KNKID"
     gene            1033..1515
                     /locus_tag="VNU03_11620"
     CDS             1033..1515
                     /locus_tag="VNU03_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWP75157.1"
                     /translation="MNRRLTLSASVIVIGGLALGLAAGCASKSMSKMGSGDVVADRQR
                     LMKAVGANWADIQAKAKAGNIEGIAVNAETLALSAQHIPSLFPEGSGTEKSKAKPDIW
                     QKWPEFEKAAKNLEAQSEKLRDASKAKNEQLTQDIVKDFGRNTCGICHTPFRQPPPRT
                     "
     gene            1605..2669
                     /locus_tag="VNU03_11625"
     CDS             1605..2669
                     /locus_tag="VNU03_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75158.1"
                     /translation="MTRNFKVLVVDDEPDIVRYLEHVLHRLGHETASAGDGAQAIEKA
                     AVDPPDLILLDVMMPVMDGFAVCRSLREREATRLIPVIVMTALEGIENRVKLIEHGAD
                     DVLAKPVDERELVARVQGALTRKRELDQRLGELTRVRDHFSKFVPEAVRRLVLANPDA
                     PRLDKQERDVTVLFVDISGYSRLAERVAPADLNALVERYFGSFLDRITDAGGDINETS
                     GDGFMAIFQDGDPAAHAARTVNAALMLLALTEALNQTELEHPLAIHIGINSGLALVGS
                     TRFEGRRGTRWTFTASGIVTTLAARLASSAGDGEILAGPETVRRLGGRYRLERVGREM
                     LKNLTEAIEVHRIISPPSRR"
     gene            complement(2923..3582)
                     /locus_tag="VNU03_11630"
     CDS             complement(2923..3582)
                     /locus_tag="VNU03_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWP75159.1"
                     /translation="MPHRRFLTATALSLLALLGGCSTAVVTTPPTPAEPPAAGNEEVG
                     YASWYGAQHQGKRTASGEAYDMNQLTAAHRTLPFGTRLLVTNRDTSQSAEVRVNDRGP
                     FVKGRILDVSYAAARQLGAVGAGIFPVRLRVIALPGTRADGPAGDGGFTVQVGAFTSR
                     ARAEALRETVGGDAVIIESTVAGETLYRVRVGSFPDRTQATPTARSLTTRGFQPLILS
                     R"
     gene            3725..3922
                     /locus_tag="VNU03_11635"
     CDS             3725..3922
                     /locus_tag="VNU03_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75160.1"
                     /translation="MRLKREDVERMMEGRPAGSTLEEALEVFEVFASGSLADEIYVLD
                     DVGGKRIAIAPAALKAKYRKE"
     gene            3929..4756
                     /locus_tag="VNU03_11640"
     CDS             3929..4756
                     /locus_tag="VNU03_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HWP75161.1"
                     /translation="MAIVTISHEIGAGGPEIGQQLAERLGCRYVDHELISNAATRYGL
                     LEEKLSHLDESKPSLFERFDAETRRYITVIQTALYDFAEQDNVVLMGRGGQWLLRGIP
                     HVLRVRVMAPFDVRVKRLTKKLAGPMGEQTNPRTVTELVRRDDAEKAGRMRYLYEVSI
                     TDPSLYDLVINTEKLSTGAAVGLLAGVVGQPELATTPASAQLVADRSLASRVQVALAT
                     HPETRKYRITVEARSGVVTLEGTAAMDEAMDVARSVEGVRDVKARQVDIPPIPPFVA"
     gene            complement(4775..5971)
                     /locus_tag="VNU03_11645"
     CDS             complement(4775..5971)
                     /locus_tag="VNU03_11645"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWP75162.1"
                     /translation="MSRRLVLISVVAALLIAAAGGGYLVRGHLGRPSASPVPTTPQAA
                     TGVALAEALQMGFVDVAQQVRPAVVHLGTIQRAKSRRGPNLPQGNDDPFFRDFFNQFF
                     GSEGPDSRSEFRRPGLGSGVIIDKRGLVLTNFHVVKGADEILIRLSDKREYRGQIMGT
                     DPKTDLAVVKFQPDHEITVATLGNSDALRVGEWAIAIGNPFGLDQTVTVGVISATGRS
                     DVGIATYENFIQTDASINPGNSGGPLVNLRGQVIGVNTAIVAAGQGIGFAIPINMVKR
                     VVDQLVDKGKVVRGWLGVALQPLSLDLAQSLGLEGTSGAVVGSTITGSPAAQAGLEQG
                     DVIVAFDKTPVEDYRHVQRLVAETRVGKSVTLQIVRKKQKMDVTVMIAEVPEDSGRRP
                     GPPPKN"
     gene            6085..6966
                     /locus_tag="VNU03_11650"
     CDS             6085..6966
                     /locus_tag="VNU03_11650"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWP75163.1"
                     /translation="MRYRHVWPGVLLLAALALLLVGLLRWGDHFLSPGPSGGIHRLLQ
                     LWAASRPPDESAAAPQIASAPSPVTDAPVSPPSPPVPVSPEPPAPPPVASAAPPPATP
                     PASASPPATAPPAAPAPAREEPRETAASAPAAPGPSAPAPVRYALALGTFAVPEDAER
                     VEAKLNQAGFSTVRFRQQVPAKLFAVLIASLKDPEEGQTTIERLQKDGYAQSVLLVGR
                     TGVTVRVGTPTPLRTAVQLAEKLRGAGYDARVATEPTRAGQVSLRHGNFASRAEADEA
                     SREVARLGVPNEVIQIR"
     gene            7016..7390
                     /locus_tag="VNU03_11655"
     CDS             7016..7390
                     /locus_tag="VNU03_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75164.1"
                     /translation="MPPKKILVVDDEPEVRKLMEHFLTSKGYDVRLAENGRLGLAALD
                     TFTPDLVLLDMHMPEMDGAETLRELAVRAPTLPVIMVTVNEDVETTSHLLQLGAADYV
                     PKPFNLDYLEQAINIQLSVTRG"
BASE COUNT         1161 a         2714 c         2746 g         1074 t
ORIGIN      
        1 cccctcggcc cgcccgtggt attttcggcg ggcgggaacc tgccggctcc atggagagtt
       61 tgatgccacg acgtggaatg tgtgtccttc gctgcgggca gctggcggcg gtactcgtcc
      121 tgatgctggc cgcaagccta ccggcggcgg cccagacccg ggagcagatc gcccgcggga
      181 aatacatctt cggagcggcc gccggctgcg gctgccacac ccttccgaag gggggagccc
      241 tcaacgcggg cgggcgcaag tatgacggtc ccttcggcac cgtgtactcg tccaacatca
      301 ccccggatcc cgccaatggc atcggcaagt ggacggacga gcaggtcatc accgcgaccc
      361 gggccgggcg gcgcccgaac ggcgagcggc tgatcccggt gcacccgtac accgtgttca
      421 acgggatggc cgagcaggac ttgaaggacg tggtcgccta cctgcgctcc gtgcctgcgg
      481 tgaacaagcc ggtaccggcc aagaagatca tgcccatgtt cgagagcgtg ttcctgccgg
      541 cctggctggc gaccttcgcg ccccgggaaa atcctcccac cgccgccccg accacgggcc
      601 aggcgcgcgg cgagtacctg acccgcgcgg tgtcgcactg cggggaatgt cacaccccga
      661 ggaccatgac catggcgacc gacaacagcc ggttcctggc cggggcggcc aagggcaagg
      721 gaccggaagg ctccgcggta cccaacatca ccccggatcg cgagaccggg ctcggcagct
      781 ggaccgagga gcagatcgcg gactatctcg agacgggcaa caagccggac ggcgacgtcg
      841 cgggcgggct gatggccgag gtcatccagg gctcctcgac gggttacaag gacctgacca
      901 aggccgatcg tgtcgcgatc gccaagtacc tcaagtcgat cccgcccatc aagaacaaga
      961 tcgactaggg ccactcttca ccagccggac cgggagccgc cggacgcggc ccggtccgcc
     1021 acaggaggca ggatgaaccg tcgactcacc ctcagcgcca gcgtgatcgt catcggtggt
     1081 ctggccctcg gcttggcagc cgggtgcgcg agcaagtcga tgtccaagat gggtagcggc
     1141 gacgtggtcg cggaccggca gcgcctgatg aaggcggtcg gcgccaactg ggccgacatc
     1201 caggcgaagg ccaaggcggg caacatcgag ggcatcgcgg tgaacgcgga gaccctcgcc
     1261 ctcagcgccc agcacatccc gtccttgttc ccggagggct cgggtaccga gaagtccaag
     1321 gccaagcccg acatctggca gaagtggccc gagtttgaga aggcggccaa gaacctggaa
     1381 gcgcagtcgg agaagctgcg cgacgcctcc aaggcgaaga acgagcagct gacacaggac
     1441 atcgtgaagg acttcggccg gaacacgtgc ggcatctgcc acaccccgtt ccgccagccg
     1501 ccgccccgga cgtagccgca cacccgcatc gctcggggcc ggcgccaccc gctccgcggg
     1561 ctgggccggc cccgcacctt gtctgctaga ctcccgccag gcggatgacg cgcaacttca
     1621 aggtgctcgt ggtcgacgac gagccggata tcgtccggta cctggagcac gtgctccacc
     1681 ggttgggcca cgagacggcc agcgcgggcg acggggccca ggccatcgag aaggcggccg
     1741 tcgatccccc cgacctgatc ctcctggacg tgatgatgcc ggtcatggac ggcttcgcgg
     1801 tctgccggag cctgcgcgag cgcgaggcga cgcggctcat cccggtgatc gtcatgaccg
     1861 cgctggaggg gatcgagaac cgggtcaagc tcatcgagca cggagccgac gacgtgctgg
     1921 ccaagccggt ggacgagcgc gagctggtgg cccgcgtcca gggggcgctc acccgcaagc
     1981 gcgagctcga ccagcgtctg ggggagctga cccgggtgcg cgatcacttc tccaagttcg
     2041 tgccggaggc ggtccggcgt ctcgtgctcg cgaacccgga cgcgccccgt ctcgacaagc
     2101 aggagcgcga cgtcaccgtg ctcttcgtcg acatcagcgg ctacagccgc ctcgcggagc
     2161 gcgtggctcc cgccgacctc aacgcgctcg tcgagcgcta cttcggcagc ttcctggacc
     2221 gcatcaccga cgcgggcggt gacatcaacg agacctccgg cgacgggttc atggccatct
     2281 tccaggacgg cgatccggcg gcccacgccg ctcgcaccgt gaacgcggcg ctcatgctgc
     2341 tggccctgac cgaggcgctg aaccagacgg agctcgagca cccgctcgcc attcacatcg
     2401 gcatcaactc cgggctggcc ctcgtcggct ccacccgctt cgagggccgg cgcggcaccc
     2461 gctggacgtt caccgccagc gggatcgtga ccacgctggc cgcgcgactc gcgtcctcgg
     2521 cgggcgacgg cgagattctc gcagggcccg agaccgtgcg gcggctcggc ggacgctacc
     2581 ggctcgagcg ggtggggcgc gagatgctga agaacctcac cgaggcgatc gaggtgcatc
     2641 gcatcatcag ccctccctct cgccgctgac ggttggtggc ctgggaccga ggggggcggg
     2701 gccgggcggg gggcacgccc tccccggaac acgctgactc gctacccggt gcgccgcctc
     2761 gggcgggcca cgagatgatc gggtgtcgct tcgctttccc gcagaggcca cggttccggg
     2821 gagggcgtgc ccccgcccgg ccccgccccc ctcgcgcgcg gcgtctcctc cggcgcgcgc
     2881 tctcgctcgc gcgtccttcc tctccattct ttcgcctgcg ggtcagcggg agaggatgag
     2941 gggttggaag ccgcgggtgg tgagggagcg ggcggtgggg gtggcctggg tgcggtcggg
     3001 gaaggagccg acgcggaccc ggtagagggt ctcgcccgcg acggtggatt cgatgatgac
     3061 cgcgtcgccg ccgaccgtct cgcgaagcgc ctcggcgcgg gcgcggctcg tgaacgcgcc
     3121 gacctggacc gtgaagcctc cgtcgccggc ggggccgtcc gcccgggtgc cgggcagggc
     3181 gatcacccgg agccgcaccg ggaagattcc cgcgcccacc gcgcccagct gccgggccgc
     3241 cgcataggag acgtccagga tccggccctt cacgaatggg ccccgatcgt tgacccgcac
     3301 ctcggccgac tggctcgtgt cccggttggt cacgagcagg cgcgtgccga agggcagggt
     3361 gcgatgggcg gcggtgagct ggttcatgtc gtaggcctcg ccgctggcgg tgcgcttgcc
     3421 ctggtgctgg gccccgtacc acgaggcgta gccgacctcc tcgttgccgg cggcgggtgg
     3481 ctctgccggc gtcggcgggg tggttaccac cgcggtggag cagccgccca gcagggccag
     3541 gagcgacagc gcggtggcgg tgaggaagcg gcgatgcggc acgtcttcta tcctgccatc
     3601 cggggggaga tcgggccagg gggcggcccg gctccggccg atcccggggc gggacccctt
     3661 gtcttgccgg ggctttcctg atactcaagg aagcccagct tccgcgagga gggagtctcg
     3721 atccgtgcgg ctcaagcgag aggacgtgga gcggatgatg gagggccggc ccgcgggcag
     3781 cacgctcgag gaggccctcg aggtcttcga ggtcttcgcc agcggcagcc tcgcggacga
     3841 gatctacgtg ctcgacgacg tcgggggcaa gcgcatcgcc atcgcccccg ccgcgttgaa
     3901 ggccaagtac cggaaggagt gacacgccat ggccatcgtc accatctccc atgagatcgg
     3961 cgcgggcggc ccggagatcg gccagcagct ggccgagcgc ctgggctgcc gctacgtgga
     4021 ccacgagctc atctccaacg cggccacccg ctacggcctg ctcgaggaga agctctcgca
     4081 cctcgacgag tcgaagccgt cgctcttcga gcgcttcgac gcggagacgc ggcgctacat
     4141 cacggtcatc cagaccgcgc tctacgactt cgcggagcag gacaacgtgg tgctcatggg
     4201 ccgcggcggg cagtggctgc tgcggggcat cccccacgtg ctgcgcgtgc gggtgatggc
     4261 gccgttcgat gtgcgggtca agcgcctgac caagaagctc gcggggccga tgggggagca
     4321 gacgaacccg cgcaccgtca ccgagctggt gcgccgcgac gacgccgaga aggccggacg
     4381 catgcggtat ctctacgagg tcagcatcac cgacccctcg ctctacgacc tcgtcatcaa
     4441 caccgagaag ctctcgacgg gggccgcggt cggtctgctc gcgggagtgg tcgggcagcc
     4501 cgagctggcc accaccccgg cgagcgccca gctggtggcc gaccgctcgc tcgcgtcgcg
     4561 ggtccaggtg gcgctcgcca cccacccgga gacgcgcaag taccgcatca cggtggaggc
     4621 ccggagcggc gtggtcaccc tcgagggcac cgcagccatg gacgaggcga tggacgtggc
     4681 gcgcagcgtg gagggggtgc gggacgtcaa ggcgcggcag gtggacatcc cgccgatccc
     4741 gccgttcgtg gcctaggcgg tcccgaccgc tcgatcagtt cttgggggga gggccggggc
     4801 ggcggccgga gtcttccggt acctcggcga tcatgaccgt cacgtccatc ttctgcttct
     4861 tgcggacgat ctggagcgtg acgctcttgc cgacccgggt ctccgcgacc agccgctgca
     4921 catgccggta gtcttccacc ggggtcttgt cgaacgcgac gatgacgtcg ccctgctcga
     4981 gccccgcctg cgcggcggga ctgccggtga tggtggagcc caccaccgcg ccgctggtgc
     5041 cctcgagccc gaggctctgc gcgaggtcga gggagagcgg ctgcagcgcg accccgagcc
     5101 agccgcgcac caccttgccc ttgtcgacca gctggtccac cacccgcttg accatgttga
     5161 tcgggatcgc gaagccgatg ccctggcccg cggccacgat ggcggtgttg accccgatca
     5221 cctggcccct caggttgacg agcggcccac ccgagttgcc cgggttgatg gacgcgtcgg
     5281 tctggatgaa gttctcgtag gtggcgatac cgacgtcgga gcggccggtg gcgctgatca
     5341 cccccaccgt gaccgtctgg tccaggccga acggattgcc gatcgcgatg gcccattccc
     5401 cgacccggag ggcgtccgag ttgcccaggg tggccaccgt gatctcgtgg tcgggctgga
     5461 acttgaccac cgcgaggtcc gttttcgggt cggtccccat gatctgtccg cggtattctc
     5521 gtttgtcgga gagacggatc aggatctcgt ccgcgccctt gacgacgtgg aagttggtga
     5581 gcaccaggcc gcgcttgtcg atgatgacgc cggacccgag accgggccgg cggaactcgg
     5641 agcgcgagtc gggaccctcg gagccgaaga actgattgaa gaagtcgcgg aagaacgggt
     5701 cgtcgttgcc ctggggcagg ttggggccgc gccgggactt cgcgcgctgg atggtcccga
     5761 ggtggacgac cgcgggccgc acctgctggg ccacgtccac gaagcccatc tggagcgctt
     5821 cggccagcgc gacgccggtc gccgcctgcg gggtggtcgg gacgggagag gcgctcggtc
     5881 gtcccaggtg accgcgcacg aggtagccgc cgcccgccgc ggcgatcagc aacgcggcga
     5941 ccacactgat cagtaccagg cggcggctca tgtctgtact ctagcagggg gcccgtgcgc
     6001 cggcgaccgc cggagcgagg gccagagcga tcgccagagc gaacggatga tagcgactgc
     6061 cgtctttcac tgtcaaggaa gccagtgaga taccgacacg tctggccggg tgttctcctg
     6121 ctcgcggccc tggcgctgct gctggtgggc ctcctgcgct ggggcgacca tttcctctcg
     6181 cccggcccga gcggagggat ccaccgcctg ctgcagctgt gggccgcttc gcgcccgccc
     6241 gacgaatcgg ccgccgcgcc acagatcgcg tccgctccgt cgccggtgac cgacgccccc
     6301 gtgagcccgc ccagcccgcc ggtcccggtg agcccggagc cgcccgcccc gccgccggtt
     6361 gcctcggccg cgcctccgcc ggccaccccg cccgcgtcgg cctcgcctcc ggccacggcg
     6421 ccacccgcgg ccccggctcc cgcgcgcgag gagccccgcg agacggcggc gtcggcaccc
     6481 gcggccccgg gtccgtcggc gcccgccccg gtgcgctacg ccctggccct cggcaccttc
     6541 gcggtcccgg aggacgccga gcgcgtggag gccaagctga accaggccgg cttctccacg
     6601 gtccgcttcc ggcagcaggt gcccgccaag ctcttcgcgg tgctcatcgc ctctctcaag
     6661 gacccggagg aggggcagac gacgatcgag cgcctccaga aggacggcta cgcccagagc
     6721 gtgctcctgg tgggccgcac cggggtgacg gtgcgggtgg gcacgcccac gccgctgcgc
     6781 accgcggtgc agctggccga gaagctgcgc ggcgcgggct acgacgcccg cgtcgccacc
     6841 gagccgaccc gcgcgggcca agtgagcctg cgccacggca acttcgcctc acgggcggag
     6901 gccgacgagg cgagccggga ggtcgcgcgt ctgggcgtcc cgaacgaggt gatccagatc
     6961 cgctgagccc gccgcacaaa ttgaccgagg aagcggggga agggtactat cgaccatgcc
     7021 ccccaagaag atcctcgtgg tcgacgacga gcccgaggtt cgcaagctca tggagcactt
     7081 cctcaccagc aagggctacg acgtccggct ggccgagaac gggcgcctcg gcctcgccgc
     7141 gctcgacacc ttcacgcccg acctggtgtt gctcgacatg cacatgccgg agatggatgg
     7201 ggccgagacg ctccgggagc tggcggtgcg cgcgcccacc ctcccggtga tcatggtcac
     7261 cgtgaacgag gacgtcgaga cgacctcgca cctgctgcag ctgggcgccg ccgactacgt
     7321 gccgaagccg ttcaatctgg actacctcga gcaggcgatc aacatccagc tctccgtgac
     7381 gcgcgggtag cgtctcgcac ggcggggggc gggggtggcc ggggggctcc ctcccgggaa
     7441 cacgctgcgt tgcatcccgc gattccgtct cgtgcgagcc acgagatagt cggcgttccg
     7501 cctcgtgtcc ccgcagaagc tacggttccc cggagggagc cccccggcca cccccgcccc
     7561 ccgccgcgcg agactgcggt tcattcacag ccgaaccttc acagccgaac cgccctcgaa
     7621 ttgccgggac ggcgcacccc gggcgggggt tagggcttcg aggtgagggt gtcgagggtg
     7681 gtgcgggcgg tgggg
//
LOCUS       DATMCV010000505         3563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_558678, whole genome shotgun sequence.
ACCESSION   DATMCV010000505 DATMCV010000000
VERSION     DATMCV010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558678"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..600)
                     /locus_tag="VNU03_11660"
     CDS             complement(355..600)
                     /locus_tag="VNU03_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75165.1"
                     /translation="MAGLGPFRTTATEAGTTEVSDGYAKSTGCPDAMAESPKKSITTP
                     TPYWPETTASGTPKLRSVMAPMLPPICRSRPQPFSQP"
     gene            complement(715..999)
                     /locus_tag="VNU03_11665"
     CDS             complement(715..999)
                     /locus_tag="VNU03_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75166.1"
                     /translation="MTSRSEARPTSSGTVPASRRASASWATAASTARPPRRAVATCAS
                     AAWITLHAARSSAWRNVIVWSKSELTTLRRPEESLSSKSTPPSFIANGFG"
     gene            complement(1127..2701)
                     /locus_tag="VNU03_11670"
     CDS             complement(1127..2701)
                     /locus_tag="VNU03_11670"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP75167.1"
                     /translation="MRHTLAALAVLLAATTASAQTSSDGQNWETPMSAGDRAYQQGRY
                     AEAERFFTTALERAERAGGAQDPRVALSLGLLASAYQARGSYGKAEPLLGRALGIAEG
                     ALGPDHPEVAVTLTALGSLGTAQGRYAQAEPLLRRALAIDERVLGPEHPEVAQTLQVL
                     AALERRQGRYVDAEVRSRRALAIREKVLGPDHVDVATSLAGLAALYRPQSRYVEAEQL
                     AQRALAIREKRLGPEHPDVIAPLVTLALVYVEQGRWAAAEPLARRALSVAEKTLGPEH
                     PDVATAIEPLAGLARERGRYAEAETLGRRELAIREKALGPEHPDVAAAVTGLAELAVL
                     RRKAAQAEPLARRALAIQEKALGPDHPDLARSLRVLGLAQDMQSRYAEAEPSFQRALA
                     IDEKRLGPESPRVALDLGGLGTLYRRQGRFAQAEPVFRRAQAIGEKVLGPEHPQVAAS
                     IVDLASLYRDQSRFAQAEPLYQWALTLQRKSLGPDHPDVAESLEEYAVLLKQTNRETE
                     AAPLEAQAKAIRAKTQ"
     gene            complement(2704..>3563)
                     /locus_tag="VNU03_11675"
     CDS             complement(2704..>3563)
                     /locus_tag="VNU03_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWP75168.1"
                     /translation="RPKAAAPAAPPAVAPPPPSTQWAVIIGVGGYESTAVPKLRYPVA
                     DADAIYQTLIASGGFKKENILLLTDKTERKPTLRNIKWALGTFLARSAHKDDLVVIYF
                     AGHGASEVDQRGVERDGLSKYLVPSDADPDDLYSSALPMDEMQNVLSRIEAERITVFL
                     DACYSGAAGGRTFASTKTRAVNVDDIFLERLTRSKGRAIVTASRPSEVSVELPELGHG
                     LFTYYLVRGLQGYADLNRDGIVSLQELYEYLTQEVSRKSRAVGGNQHPMMKGELEGAL
                     PLTKAGKRN"
BASE COUNT          438 a         1283 c         1329 g          513 t
ORIGIN      
        1 cgagacgcgg gcccggccgg aggccgacgt cttctgggtc tccgcggtcg acgccttcca
       61 gaccctcaag gccgacggcc tgctcgaccg gatcatcctg ccgcgggaga cgctggcgcg
      121 catgccgccg cggatcggga gcttcccgct ccacgacccc gacggcacct actggggctt
      181 cgcggtctcg ggctacgggc tcatgtggaa cacgcgctac ctcgcgctcc acaagctgcc
      241 cgcgccgcgc gagtggaccg atctcgccga cccgcgctac gccggccatc tcgtcatctc
      301 ggccccgtcg cgcagcggca ccacgcacct caccgtcgag gtgatcctga aggcctacgg
      361 ctgggagaaa ggctggggcc tgctcctgca gatgggcggc aacatgggcg ccatcaccga
      421 gcgcagcttc ggggtgccgg aggcggtggt ctccggccag tacggcgtcg gggtggtgat
      481 cgacttcttc gggctctcgg ccatcgcgtc cgggcagccg gtcgacttcg cgtacccgtc
      541 gctcacctcg gtggttccgg cctcggtggc ggtggtccgg aacgggccca atcccgccaa
      601 cgcccgcgcc ttcgtcgagt acctgctgag cgacgagggc cagcagaagc tcttcgcgcc
      661 cgacatcggg cggcttccgg tggtgcccgc gctctacgcg cgcgcgccga agggctaccc
      721 gaatccgttc gcgatgaagc tcggcggggt cgacttcgac gacaagctct cctccggccg
      781 ccgcaacgtg gtcaactcgc tcttcgacca cacgatcacg ttccgccacg ccgagctgcg
      841 cgccgcgtgg agcgtgatcc acgccgccga ggcgcaggtg gcgaccgcgc gccgcggcgg
      901 ccgcgcggtc gacgcggcgg tggcccagct cgcggaggcg cgccggctcg cggggacggt
      961 gccgctcgac gtggggcgcg cctccgaccg cgaggtcaac gccgcgttcc gcgacaagcc
     1021 ggacgtgaag tcgcgcctgg agaccgagtg ggacagctcg gccaaggccg cctacgccag
     1081 ggcccgcgag ctggccgagt ccgcccccgc tcgcatccgc cgccggctat tgcgtcttgg
     1141 cgcggatggc cttggcctgc gcctcgagcg gcgcggcctc cgtctcgcgg ttggtctgct
     1201 tgaggagcac cgcgtactcc tcgaggctct cggccacgtc gggatggtcg gggccgaggg
     1261 acttgcgctg gagcgtgagc gcccactggt agaggggctc ggcctgcgcg aagcggctct
     1321 ggtcgcgata gagcgaggcg aggtcgacga tgctcgcggc cacctgcggg tgctcgggtc
     1381 cgagcacctt ctctccgatc gcctgggcgc gccggaagac gggctcggcc tgggcgaagc
     1441 ggccctggcg gcgatagagg gtgccgagcc cgcccaggtc gagggcgacg cgcggcgact
     1501 cgggcccgag ccgcttctcg tcgatcgcga gcgcgcgctg gaacgacggc tccgcctccg
     1561 cgtaccggct ctgcatgtcc tgggcgaggc cgaggacgcg caggctccgg gccaggtccg
     1621 ggtgatccgg cccgagcgcc ttctcctgga tggcgagcgc gcgccgggcg agcggctcgg
     1681 cctgcgccgc cttccggcgg agaaccgcga gctcggccag cccggtcacc gccgccgcca
     1741 cgtcgggatg ctcgggcccg agggccttct cgcggatcgc gagctcacgc cgccccaggg
     1801 tctcggcctc cgcgtagcgg ccgcgctcgc gcgcgaggcc ggcgagcggc tcgatcgcgg
     1861 tggccacgtc gggatgctcg gggccgagcg tcttctcggc caccgacagc gcgcggcggg
     1921 cgagcggctc ggcggccgcc caccggccct gctcgacgta gaccagagcc agcgtgacga
     1981 ggggcgcgat cacgtcgggg tgctcggggc cgaggcgctt ctcgcggatg gcgagcgcgc
     2041 gctgcgcgag ctgctccgcc tcgacgtagc ggctctgcgg gcggtagagc gcggcgagcc
     2101 cggcgaggct cgtcgccacg tccacgtgat cgggccccag caccttctcg cggatcgcga
     2161 gcgcccggcg gcttcgcacc tccgcgtcga cgtagcgccc ctgccggcgc tcgagcgcgg
     2221 ccagtacctg tagcgtctgg gcgacctcgg gatgctccgg gccgaggacg cgctcgtcga
     2281 tggcgagcgc gcgccggagc agcggctcgg cctgcgcgta gcgtccctgc gcggtcccga
     2341 ggctgccgag cgcggtgagg gtcaccgcga cctcgggatg gtccggcccg agcgcgccct
     2401 cggcgatgcc gagggcgcgg ccgagcaggg gctccgcctt gccgtagctg cccctcgcct
     2461 ggtaggccga ggccagcagc ccgaggctca aggccacccg cgggtcctgc gccccgccgg
     2521 cccgctccgc gcgctccagc gcggtcgtga agaagcgctc ggcctcggcg tagcggccct
     2581 gctggtaggc gcggtcgccc gccgacatgg gcgtctccca gttctgtccg tcggaggagg
     2641 tctgggccga ggcggtcgtg gcggccagca ggaccgcgag cgcggcgagc gtgtgacgca
     2701 cggtcagttg cgcttgccgg ccttcgtcag cggcagcgca ccttcgagct cgcccttcat
     2761 catggggtgc tggttgccgc ccaccgcccg cgatttccgg ctgacttcct gggtgaggta
     2821 ctcgtagagc tcctgaaggg agacgatgcc gtcccggttg agatcggcgt atccctgcag
     2881 gccgcgcacc aggtagtaag tgaagagtcc gtggcccagc tcgggcagct ccaccgacac
     2941 ctcggacggc cgcgaggcgg tcacgatcgc gcggcccttc gagcgggtga gtcgctccag
     3001 gaagatgtcg tccacgttga ccgcgcgcgt cttggtcgag gcgaaggtgc ggccgcccgc
     3061 cgcgccgctg tagcaggcgt cgaggaagac ggtgatccgc tcggcctcga tgcgggacag
     3121 gacgttctgc atctcgtcca tcggcagagc gctcgagtag agatcgtcgg gatcggcgtc
     3181 gctcggcacc aggtacttgg agagtccatc ccgctcgacc ccgcgctggt cgacctcgga
     3241 ggcgccgtgg cccgcgaagt agatcaccac caggtcgtcc ttgtgcgccg agcgcgccag
     3301 gaacgtgccg agcgcccact tgatgttgcg cagggtgggc ttgcgctcgg tcttgtcggt
     3361 gagcaggagg atgttctcct tcttgaagcc gccggaggcg atcagggtct gatagatcgc
     3421 gtcggcgtcc gccaccggat agcggagctt cggcaccgcg gtggactcat agccgccgac
     3481 gccgatgatc accgcccact gggtggaggg cggcgggggc gcgaccgcgg gcggggccgc
     3541 gggcgcggcc gccttcggcc gct
//
LOCUS       DATMCV010000506         2415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_690511, whole genome shotgun sequence.
ACCESSION   DATMCV010000506 DATMCV010000000
VERSION     DATMCV010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690511"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1103)
                     /locus_tag="VNU03_11680"
                     /pseudo
     CDS             complement(1..1103)
                     /locus_tag="VNU03_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167361.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
     gene            complement(1156..1665)
                     /locus_tag="VNU03_11685"
     CDS             complement(1156..1665)
                     /locus_tag="VNU03_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009572143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HWP75169.1"
                     /translation="MSAPMPPDNLNEIGVLKRREIEARILMPVLTALGEEFGREKVFE
                     TARRIIIDVAREQGKELAGRMGGDSLGHFATALEDWKKGDAYRMDVLEQSEEKFSFNV
                     TRCRYAEMYRALGIPEIGALLSCNRDFSLVEGFNPAVKLTRTQTVMEGASHCDFRFEL
                     RRPRRDATE"
     gene            complement(1683..2117)
                     /locus_tag="VNU03_11690"
     CDS             complement(1683..2117)
                     /locus_tag="VNU03_11690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWP75170.1"
                     /translation="MTTAAEELRFNHSPFMRFMGLEMVRAEKGLVEIRLPFREEFLRH
                     DGADWLHGGVVSALADIAGDYAVITETSAGVPTIDMRVDYLRPARRGDLMAIGRTVRV
                     GKTVSVADVEIRDASGTLVAVGRACYASPRTPPDATRPKDSA"
     gene            complement(2114..>2415)
                     /locus_tag="VNU03_11695"
     CDS             complement(2114..>2415)
                     /locus_tag="VNU03_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HWP75171.1"
                     /translation="SGENVYPKEVEDILLQHPNLRDACVVPAPHDVKGEVPIAFVVAR
                     EAGRTTEDDVRRFFLERGAPYAHPRKVVFLDALPLGGTGKIDRTALKARAREMGS"
BASE COUNT          345 a          900 c          819 g          351 t
ORIGIN      
        1 cgcgccagga cctccggccg cggaaacgcg tgggtcagtt cgtggtcggg ctccgccagg
       61 ggctccccgt aagcgacgac catgcgcccg gcaagacaga ccgcgtcggc gacggagatc
      121 tcctccccca gcaccgcgca catcgccaat tcgaagccgt cccacgcgcc gggcacccgc
      181 aggcccggcc gcgccgccac caggggcgcc agcgccggat cctttgcgag gtgggcggag
      241 atcggaagcg gatcggcggc caggtcgaac acgcggcgca gccgcgcgat gatgccgggc
      301 agcgcggaga gcctggggaa tcggatcctc gcgcggagcg cgtttccagc gtccggtcga
      361 acggacacga tcccctgtcg gccgtccagc tggatggctc gcgaatagca gctccccacc
      421 acccgctcga cgccgggcac ggcgtggagc cgcaggcact ccagcatgcc cggccagtcg
      481 tagggcggct gatagcgcag gagcaggctg atctcgcctc ccggacccgc cgggatctcc
      541 ggtcagcccc tgcgccgcag ggcgcacggt gcacggccgt acagctccgt gaacgtctcg
      601 ttgaaccggc ggatgctccc gaagcccgcc gcgaacgcga tctccgccat cggcagcgcc
      661 gtctcgtgaa tgagctgctt ggccagcagc acgcggcggg tctgggcgac cgcgatcggt
      721 gacgcgccga gatgctggcg acagaggcgc cggagctgcc gctcacccag cccgaggcgg
      781 ccggccaggg cgtcgacgct cccctcgtcc agggctccca gctcgatgag cgcgagcgcg
      841 cgggacaccg tgttcgaggt tccccgccac gcgcccttgt ccggtgccgt ctccggccgg
      901 caccgcaagc aggggcggaa ccccgcctcc tgtgccgcgg ccgccgtggg ataaaacgtc
      961 acgttctccg agcgcggcgt gcgcgccgga cagaccgggc ggcagtaggt accggtcgtc
     1021 cttaccgcgg tgaagaagcg gccgtcgaag cgggcgtcgc gcagctcgac cgcgcggtag
     1081 caggcgtcgt gatcgagatc catgccgcga tactggcccg gagaaccgcc ggtgtctggc
     1141 ggttttcgga cgtcgtcatt ccgtggcgtc gcgcctcggc cggcgcagct cgaagcggaa
     1201 gtcgcagtgc gacgccccct ccatcacggt ctgcgtgcgg gtcagcttca ccgccgggtt
     1261 gaagccctcg accagggaga agtcgcggtt gcacgagagc agcgcgccga tctccgggat
     1321 gccgagcgcg cggtacatct ccgcgtaccg gcagcgcgtg acgttgaacg agaacttctc
     1381 ctcgctctgc tccagcacgt ccatgcggta ggcgtcgccc ttcttccagt cctccagcgc
     1441 ggtggcgaag tggccgaggc tgtcgccgcc catgcgcccg gccagctcct tgccctgctc
     1501 gcgcgccacg tcgatgatga tccggcgcgc ggtctcgaac accttctcgc gcccgaactc
     1561 ctcgcccagc gcggtcagca ccggcatcag gatgcgcgcc tcgatctcgc ggcgcttgag
     1621 cacgccgatc tcgttcaggt tgtcgggggg cattggggca ctcatcggat ctcctccttg
     1681 ggtcacgccg aatccttggg tcgcgtggcg tccggcggag tgcgcggcga agcgtagcac
     1741 gcgcgcccca ccgcgaccag ggtgccgctc gcgtcgcgga tctccacatc ggccacgctc
     1801 accgtcttgc ccacccgcac cgtgcgcccg atggccatca ggtcgccccg gcgggccggc
     1861 cgcaggtagt ccacccgcat gtcgatggtg ggcacgcccg cgctcgtctc ggtgatcacc
     1921 gcgtagtccc ccgcgatgtc ggccagcgcg ctgaccaccc cgccgtgcag ccagtccgcg
     1981 ccgtcgtggc gcaggaactc ctcgcggaac ggcagccgga tctccaccag gcccttctcg
     2041 gcccgcacca tctcgagccc catgaagcgc atgaacggcg agtgattgaa gcgcagctcc
     2101 tcggccgccg tcgtcacgag cccatctccc tcgcccgcgc cttgagcgcg gtgcggtcga
     2161 tcttgccggt gccgccaagc ggtagcgcgt ccaggaacac cactttccgc ggatgggcgt
     2221 agggggcccc gcgctccagg aagaagcggc gcacgtcgtc ttccgtggtc cggcccgcct
     2281 cgcgggccac gacgaacgcg atcggcacct cgcccttgac gtcgtggggc gccggcacca
     2341 cacaggcgtc tcgcaggttc ggatgctgga gcaggatgtc ctccacttcc ttggggtata
     2401 cgttctcgcc cgaca
//
LOCUS       DATMCV010000507         5021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_24661, whole genome shotgun sequence.
ACCESSION   DATMCV010000507 DATMCV010000000
VERSION     DATMCV010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24661"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VNU03_11700"
     CDS             <1..670
                     /locus_tag="VNU03_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75172.1"
                     /translation="DREGMAKQLLKGTAVAARGLQAPCCPSYDPNFKDYPYDPAQAKK
                     LLAEAGFPNGFETVWQTSVDGSGQLIPVPMAEWIQRDLAKIGVRMKLRTYEWITYIGY
                     WIKGMDDASNKVGGQQMSWGMTSDYWLDIVTNSSHIAPAGGAGQNGGYYTGADAMLNA
                     ARGEMDEGKRATLYRKINQKIKEDAPAVPIINDLAPLMLSSKVKGFVHAPEEWYDMTT
                     VWLA"
     gene            721..1674
                     /locus_tag="VNU03_11705"
     CDS             721..1674
                     /locus_tag="VNU03_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75173.1"
                     /translation="MLKFLVYRVATIVLVLFGVSIVVFLMMKLIPGDPAEAILGPAAT
                     TEKIEMVRKSLGLDQPFYVQYVRWLWKVLQGDFGTSIAVNVPVAQLVWPKFWNTVLLT
                     AGSLVICIVIGLGVGIVSGTRQYSWFDRISMSLTLIVANTPAFWLGLILMVVLALYLG
                     WFPATGMYSMRASQKTFGDLIHHMVLPAITTASVSAAIIARLVRSSFLDVIRKHYITA
                     LRAKGLSERDIILRHGMKNALPPIINIVGLQVGYLMGGALFTEVVFSWPGIGLQIYSS
                     IVARDLPVVQAAVLVTTFVFVLVNLGTDVVVAALDPRIRRA"
     gene            1686..2618
                     /locus_tag="VNU03_11710"
     CDS             1686..2618
                     /locus_tag="VNU03_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75174.1"
                     /translation="MSVVNLPLKDEPVDGRALPLDAAEAQMFRRPSEWRIFWEAFSRD
                     RLAVGAAALIGLVCLISALASVLSPYDPTVGDNALRLAPIGTPGHLLGLDGQGRDILS
                     RLIWGGQVSIIIALLPVTVASLISVLLGLVAGFFGGKVDMVIMRTLDVFFAFPAVLLA
                     VAIAGVLGPGMLNVMLSLTIVLIPYISRVAYMATVTVKSELFVDQARVSGARPSQILF
                     HHILPNTLSPVIVYGSTLVGILIVLGAGLSFLGLGITPPRPDWGIMCSDGRNFLSVAP
                     HVSAVPGIMLILVAMSFNIVGDGLRDALDPKLRK"
     gene            2627..3595
                     /locus_tag="VNU03_11715"
     CDS             2627..3595
                     /locus_tag="VNU03_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75175.1"
                     /translation="MSEALLEVDDLHTHFHLKNKTVRAVNGVSFAINRGEIVGIAGES
                     GAGKSVACLSILRLVPSPGRIERGRILFSGEDLLTKSDRQMRDLRGQTVSMVFEDPLN
                     SLDPAYTVGSLIGEALILHKGLSKREARTKAGDLLRLVGIPDPERRLDNYAHELSGGM
                     QQRVMIAIALSCDPALLIADNPTTALDVTIQIQILDLVKQLRDRLGMGVLWITHDLGV
                     VAKLCDRVVIMYAGEVMEQGRVYDVLKHPRHPYTRRLIDALPKLGRPDQRLMPIEGRP
                     PDPANLPAGCPFAPRCHRARPECEAGDVPLVETGDGHAVRCLFPEE"
     gene            3597..4667
                     /locus_tag="VNU03_11720"
     CDS             3597..4667
                     /locus_tag="VNU03_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75176.1"
                     /translation="MTIAAPPEAPKLLPKLLEVKNLVKHFPVRRGLWASGPPAAVRAV
                     DGISFDIGRGETVGLVGESGCGKTTTGRMIMKLIDPTSGDIRFEGKSIGGIPFEEERA
                     YRRHVQMVFQDPYSSLNPRMTVGSIIGYPMRIQNAYPGPARDARIRELLELVGLSRYQ
                     ASWYPHEFSGGERQRVGIAAALALNPKFIFCDEPVSSLDVSAQAQILNLLKDVRREFN
                     LTILIVTHNLSVVEYLSDRVVVMYLGKIVEVASTADLYRETLHPYSKALVSAIPTPDP
                     DVEREREQIILRGEIPSPLNPPTGCRFHTRCQAYIGDVCRDVEPELREVRPGHFTACH
                     LYSGLTAAPAALGASRAAGQLV"
     gene            4687..>5021
                     /locus_tag="VNU03_11725"
     CDS             4687..>5021
                     /locus_tag="VNU03_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP75177.1"
                     /translation="MPVSLPTPDQLKRIAEKMNLSLTDADVASFLGLMKPSIDGYNVV
                     DQLPDNLPPVKYPRTPGYRPQPEENKHNAWYYKSKIEGAPQGKLKGKRVVIKDNVMVA
                     GVPMMNGAST"
BASE COUNT          780 a         1747 c         1690 g          804 t
ORIGIN      
        1 cgaccgcgag ggcatggcca agcagctcct gaaggggacc gcggtcgccg cgcgaggtct
       61 gcaggcgccg tgctgcccgt cctacgatcc gaacttcaag gactatccct acgaccccgc
      121 gcaggccaag aagctcctcg ccgaggccgg cttcccgaac ggcttcgaga cggtgtggca
      181 gacgtcggtc gacggcagcg gccagctcat cccggtgccg atggcggagt ggatccagcg
      241 cgacctcgcc aagatcggcg tccggatgaa gctccgcacc tacgagtgga tcacctacat
      301 cggctactgg atcaagggca tggacgacgc ctccaacaag gtcggcggcc agcagatgag
      361 ctgggggatg acgagcgact actggctcga catcgtcacc aattcttccc acatcgcccc
      421 cgcgggcggg gcgggccaga acggcggcta ctacaccggc gccgacgcga tgctgaacgc
      481 ggcccgcggc gagatggacg aggggaagcg cgccaccctg taccgaaaga tcaaccagaa
      541 gatcaaggaa gacgcccccg cggtgcccat catcaacgac ctcgcgccgc tgatgctgag
      601 ctccaaggtg aagggcttcg tccacgcgcc ggaggagtgg tacgacatga ccacggtctg
      661 gctcgcgtga gtccccgcgt cggcccggga ggcgtaggcc tcccgggctg acctctcgcc
      721 atgctcaaat tcctcgtcta ccgcgtcgcg acgatcgtgc tcgtcctgtt cggcgtgtcg
      781 atcgtggtgt tcttgatgat gaagctgatc ccgggcgatc ccgcggaggc gatcctgggc
      841 cccgccgcca ccaccgagaa gatcgagatg gtgaggaagt cgctcgggct ggaccagccg
      901 ttctacgtgc agtacgtgcg ctggctgtgg aaggtcctcc agggcgactt cggcacctcg
      961 atcgcggtca acgtgccggt ggcgcagctg gtgtggccga agttctggaa cacggtgctg
     1021 ctcaccgcgg gcagcctggt gatctgcatc gtgatcggcc tcggggtggg catcgtctcc
     1081 gggacccgcc agtactcgtg gttcgaccgg atcagcatgt ccctgaccct catcgtcgcc
     1141 aacacccccg cgttctggct gggcctcatc ctcatggtgg tgctggccct ctatctcggc
     1201 tggttcccgg ccaccgggat gtactcgatg cgggcctcgc agaagacctt cggcgacctg
     1261 atccatcaca tggtgctgcc cgcgatcacc accgcctcgg tgtcggccgc gatcatcgcg
     1321 cgtctagtcc gctcgtcttt tctggatgtc atccgcaagc actacatcac cgcgctgcgg
     1381 gccaaggggc tgtccgagcg cgacatcatc ctgcgccacg ggatgaagaa cgcgctgccg
     1441 ccgatcatca acatcgtggg cctccaggtc ggctacctga tgggcggggc gctgttcacc
     1501 gaggtggtct tctcgtggcc gggtatcggg ctgcagatct acagctcgat cgtggcccgc
     1561 gacctgccgg tggtccaggc cgcggtcctg gtgaccacct tcgtgttcgt gctggtcaat
     1621 ctcgggaccg acgtcgtcgt cgcggccctc gatccccgaa tccgtcgggc ctgaggaggc
     1681 gcgcgatgag cgtcgtgaat ctgcccctga aggacgagcc ggtcgatggg cgggcgctgc
     1741 cgctcgacgc ggcggaggcc cagatgttcc gccggccgtc ggagtggcgg atcttctggg
     1801 aggccttcag ccgcgaccgc ctggcggtgg gcgccgcggc gctgatcggg ctcgtgtgcc
     1861 tgatctcggc gctggcctcg gtgctgagcc cctacgaccc gacggtgggc gacaacgccc
     1921 tgcgcctggc ccccatcggc acgccggggc acctgctggg cctggacggg caggggcgcg
     1981 acatcctgag ccggctcatc tggggcggcc aggtctccat catcatcgcg ctgctgccgg
     2041 tgacggtggc gagcctgatc agcgtgctcc tgggcctggt ggcggggttc ttcgggggca
     2101 aggtcgacat ggtgatcatg cgcaccctcg acgtgttctt cgccttcccg gcggtgctgc
     2161 tggcggtggc gatcgcgggg gtgctgggcc ccggcatgct caacgtgatg ctctcgctca
     2221 ccatcgtgct gatcccgtac atctcgcggg tcgcctacat ggccaccgtc accgtgaaga
     2281 gcgagctgtt cgtggaccag gcgcgggtct cgggggcgcg gccgagccag atcctcttcc
     2341 accacatcct gcccaacacg ctctccccgg tgatcgtcta cggcagcacc ctggtgggca
     2401 tcctgatcgt gctcggggcg gggctctcct tcctcgggct cggcatcacc ccgccccggc
     2461 cggactgggg catcatgtgc tcggacgggc gcaacttcct ctcggtggcg ccccacgtct
     2521 cggcggtgcc cgggatcatg ctgatcctgg tcgccatgtc gttcaacatc gtcggggacg
     2581 gcctgcggga cgcgctcgac ccgaagctgc gcaagtgagc cccgccatga gcgaggcgct
     2641 tctcgaggtc gacgacctcc acacccactt ccacctcaag aacaagacgg tgcgggcggt
     2701 gaacggggtg tcgttcgcga tcaaccgcgg ggagatcgtc gggatcgcgg gggagtcggg
     2761 ggcgggcaag agcgtggcct gtctctcgat cctgcgactc gtcccgagcc cgggacggat
     2821 cgagcgcggc cggatcctct tctccggcga ggatctgctc accaagagcg accggcagat
     2881 gcgggacctg cggggccaga ccgtctcgat ggtgttcgag gacccgctga actccctgga
     2941 cccggcctac accgtcggca gcctgatcgg ggaggcgctg atcctccaca agggcctgtc
     3001 caagcgcgag gcccggacca aggcggggga tctgctgcgg ctggtgggca tcccggaccc
     3061 ggagcgtcgg ctcgacaact acgcccacga gctgtcgggc ggcatgcagc agcgggtgat
     3121 gatcgcgatc gcgctctcct gcgacccggc gctgctgatc gcggacaacc cgaccaccgc
     3181 gctcgacgtg accatccaga tccagatcct cgacctcgtg aagcagctcc gcgaccggct
     3241 cggcatgggg gtgctgtgga tcacccacga cctcggcgtg gtggccaagc tctgcgaccg
     3301 ggtggtgatc atgtacgcgg gcgaggtcat ggagcagggc cgggtgtacg acgtgctgaa
     3361 gcacccgcgg catccctaca cgcgccggct catcgacgcg ctgcccaagc tcgggcgccc
     3421 cgaccagcgg ctcatgccga tcgaggggcg cccacccgat cccgcgaacc tgcccgccgg
     3481 atgtcccttc gcgccgcgct gccaccgggc gcgcccggag tgcgaggcgg gcgacgtgcc
     3541 gctcgtcgag accggcgacg ggcacgcggt tcgctgcctg ttcccggagg agtgagatga
     3601 cgatcgccgc gccccccgag gcgcccaagc tgctgcccaa gctcctggaa gtcaagaacc
     3661 tggtgaagca cttcccggtg cgccgcgggc tctgggcgag cggcccgccc gccgcggtgc
     3721 gcgcggtcga cggcatcagc ttcgacatcg ggcgcggcga gacggtcggg ctggtgggcg
     3781 agtccggctg cggcaagacc accaccgggc ggatgatcat gaagctgatc gacccgacct
     3841 cgggggacat ccgcttcgag ggcaagtcga tcgggggcat cccgttcgag gaggagcgcg
     3901 cctaccgccg ccacgtgcag atggtcttcc aggatccgta ctcgtcgctg aacccccgga
     3961 tgacggtggg ctcgatcatc ggctacccga tgcggatcca gaacgcgtac cccgggcccg
     4021 cccgcgacgc ccgcatccgc gagctgctgg agctggtcgg cctctcgcgc taccaggcga
     4081 gctggtaccc gcacgagttc agcgggggcg agcgccagcg ggtgggcatc gcggccgccc
     4141 tggccctcaa ccccaagttc atcttctgcg acgagccggt ctcctccctc gacgtctcgg
     4201 cccaggcgca gatcctgaac ctcctgaagg acgtccgccg cgagttcaac ctcaccatcc
     4261 tcatcgtgac ccacaacctg agcgtggtgg agtatctgag cgatcgggtg gtggtgatgt
     4321 acctcggcaa gatcgtcgag gtggccagca ccgccgatct ctaccgcgag acgctccacc
     4381 cctacagcaa ggccctggtc tccgcgatcc ccaccccgga ccccgacgtg gagcgcgagc
     4441 gggagcagat catcctgcga ggggagatcc cgagcccgct caatccgccc accgggtgcc
     4501 gcttccacac ccggtgccag gcctacatcg gggacgtctg ccgagacgtc gagccggagc
     4561 tgcgcgaggt gcgccccggc cactttaccg cctgtcacct ctactccggg ctcaccgcgg
     4621 cgccggccgc gctcggggcg tcccgcgccg ccggccagct cgtctagcgt caaggaggac
     4681 accaccatgc ccgtctcgct gcccacccct gaccagctca agcgcatcgc cgagaagatg
     4741 aacctctcgc tcaccgacgc cgacgtggcg tcgttcctcg gcctcatgaa gccgagcatc
     4801 gacggctata acgtcgtcga tcagctcccg gacaacctgc cgccggtgaa gtacccccgc
     4861 acgccggggt accggcccca gccggaggag aacaagcaca acgcgtggta ctacaagtcg
     4921 aagatcgagg gcgcgcctca gggcaagctc aagggcaagc gtgtcgtgat caaggacaac
     4981 gtgatggtgg ccggggtgcc gatgatgaac ggggcctcga c
//
LOCUS       DATMCV010000508         2660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_94931, whole genome shotgun sequence.
ACCESSION   DATMCV010000508 DATMCV010000000
VERSION     DATMCV010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94931"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VNU03_11730"
     CDS             complement(<1..201)
                     /locus_tag="VNU03_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75178.1"
                     /translation="MLGRRELLKLAGVGAAAAMTGAPAVLRAQAKGKVRLAYLQLGWA
                     ATEIIHKEDLLGRHGWSAEYSVV"
     gene            complement(207..1589)
                     /locus_tag="VNU03_11735"
     CDS             complement(207..1589)
                     /locus_tag="VNU03_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HWP75179.1"
                     /translation="MARKPVDMTTKYLLSEKELPTRWYNIQADFKTPLPPPLHPGTGQ
                     PIGPQDLAPLFPMELIKQEVSQERWIEIPDQVRDILRLWRPTPLYRARRLEKALDTPA
                     RIYYKYEGASPAGSHKLNTAAAQAYYNKMEKVARLSTETGAGQWGSALAQACAFFGLQ
                     CKVYMVKVSYQQKPYRRILMETFGAQVVASPSPDTQAGKKILEQDPDSPGSLGIAISE
                     AVEDAATRDDTKYSLGSVLNHVLMHQTVIGLETKKQLERADDDADVLVGCIGGGSNFA
                     GFAFPFAGDKLTGKNKKRRIVAIEPSACPSLTKGKYAYDFGDTVGLTPLVKMHTLGHT
                     FMPAPVHAGGLRYHGMAPLICKLYDEGVIEARAVHQIATFQAAVQFGRAEGILPAPEA
                     AHAVRGAIDEALMAKKEGKRKTIVFNLSGHGFLDLSAYEAYLAGKLQDYEYPSEKVAE
                     ALKHLPKVKG"
     gene            complement(1679..>2660)
                     /locus_tag="VNU03_11740"
     CDS             complement(1679..>2660)
                     /locus_tag="VNU03_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP75180.1"
                     /translation="GRALEQLGHTVTFAENGRQALEILGQRRVDLVLLDIEMPEMDGY
                     QVLAALGADPRLRDLPVVMMSSVEEVDSVARCIEMGAEDYLFKPVNPVLLRARVGASL
                     EKKRLRDQQRELFRKFATAEVAEELLTTGVALGGKQVEASVMFSDIRGFTRITEALAP
                     ADTIELLNSYYTLTFDAIGGHGGIVNQMLGDGLMAIFGAPLPRADHRDRAVRAALEML
                     ELMDGFNQDQARRGATEVRIGIGIASGPIVAGFTGTQQRMTYTGVGDTVNLAAHLEAH
                     TKVLGHPILIDENTRAGLAAGIAVASVGPAQFKTRRQAVEVFSVTPPARP"
BASE COUNT          352 a          898 c          965 g          445 t
ORIGIN      
        1 gacgacggag tactccgcgc tccagccgtg acgcccgagc aggtcctcct tgtggatgat
       61 ctcggtggcg gcccagccga gctggaggta ggccaggcgg accttgccct tggcctgggc
      121 gcgcaggacc gcgggcgcgc cggtcatcgc ggcggccgcg cccacgccgg ccagcttgag
      181 cagctcgcgg cggccgagca cggcggctag cccttcacct tcggcaggtg cttgagggcc
      241 tccgcgacct tctcgctggg atactcgtag tcctggagct tgccggccag gtaggcctcg
      301 taggcggaga ggtcgaggaa gccgtggccg gagaggttga acacgatggt cttccgcttg
      361 ccttccttct tggccatgag cgcctcgtcg atcgcgcccc gcaccgcgtg ggccgcctcc
      421 ggggcgggca ggatcccctc cgcgcggccg aactgcaccg cggcctggaa ggtggcgatc
      481 tggtgcaccg cgcgcgcctc gatcacgccc tcgtcgtaga gcttgcagat cagcggcgcc
      541 atgccgtggt agcggagccc gcccgcgtgc accggcgcgg gcatgaaggt gtggccgagg
      601 gtgtgcatct tcacgagcgg ggtgagcccg acggtgtcgc cgaagtcgta ggcgtacttg
      661 cccttggtga ggctcgggca cgcgctcggc tcgatcgcca cgatgcggcg cttcttgttc
      721 ttgccggtga gcttgtcgcc cgcgaacggg aaggcgaagc cggcgaagtt cgagccaccg
      781 ccgatgcagc ccaccagcac gtccgcgtcg tcgtcggccc gctcgagctg cttcttggtc
      841 tcgagcccga tcacggtctg gtgcatgagc acgtggttga gcacgctgcc cagcgagtac
      901 ttggtgtcgt ctcgggtggc cgcgtcctcc accgcctcgc tgatcgcgat gccgaggctg
      961 cccggcgaat ccgggtcctg ctcgaggatc ttcttgcccg cctgggtgtc cgggctcggg
     1021 ctcgccacca cctgggcccc gaaggtctcc atgaggatgc ggcggtaggg cttctgctgg
     1081 taggagacct tgaccatgta caccttgcac tgcaggccga agaacgcgca cgcctgcgcg
     1141 agcgcggagc cccactggcc ggcgccggtc tcggtggaga ggcgggcgac cttctccatc
     1201 ttgttgtagt aggcctgggc cgccgcggtg ttgagcttgt ggctgcccgc cgggctcgcg
     1261 ccctcgtact tgtagtagat gcgcgcgggg gtgtcgagcg ccttctcgag ccggcgcgcg
     1321 cggtagagcg gagtcgggcg ccacaggcgg aggatgtcgc gcacctggtc cgggatctcg
     1381 atccagcgct cctggctgac ctcctgcttg atcagctcca tcgggaagag cggggccaga
     1441 tcttgaggtc cgatcggctg gccggtgccc ggatggagcg ggggcggcag cggggtcttg
     1501 aagtcggcct ggatgttgta ccagcgggtc gggagctcct tctcggagag gagatacttg
     1561 gtcgtcatgt cgacgggctt ccgagccatg gggcggcctc cttccaggtc aggcgacggt
     1621 gggactgatg gaaaacgggc accacactac aggtcgcgga acgaggccgt caagggggtc
     1681 agggccgcgc gggcggcgtg accgagaaga cctccactgc ctggcggcgg gtcttgaact
     1741 gggccgggcc gaccgacgcg accgcgattc cggcggcgag gccggctcgc gtgttctcgt
     1801 cgatcaggat ggggtggccg agcaccttgg tgtgcgcctc caggtgggcg gccaggttca
     1861 cggtgtcgcc caccccggtg taggtcatgc gctgctgggt gccggtgaag ccggccacga
     1921 tcgggccgga cgcgatgccg atgccgatcc ggacctccgt cgcgccgcga cgcgcctggt
     1981 cctggttgaa cccgtccatc agctcgagca tctccagtgc ggcgcgcacc gcgcggtcgc
     2041 gatggtcggc gcggggcagg ggggcgccga agatcgccat caggccgtcg ccgagcatct
     2101 ggttgacgat gccgccgtgg cccccgatcg cgtcgaaggt gagcgtgtag tagctgttca
     2161 ggagctcgat ggtgtcggcc ggcgcgagcg cctcggtgat ccgcgtgaac ccccggatgt
     2221 cggagaacat cacgctggcc tcgacctgct tgcccccgag cgcgacgccc gtcgtcagca
     2281 gctcctccgc cacctccgcg gtcgcgaact tcctgaacag ctcgcgctgc tggtcgcgca
     2341 ggcgcttctt ctccaggctc gccccgacgc gggcccggag cagcaccggg ttgaccggct
     2401 tgaacaggta gtcctccgcc cccatctcga tgcaccgcgc gacgctgtcc acctcctcga
     2461 ccgaggacat catgaccacc gggaggtcgc ggaggcgcgg gtcggcgccc agcgcggcca
     2521 gcacctggta gccgtccatc tccggcatct cgatgtcgag caggaccagg tcgacgcggc
     2581 gctggccgag gatctcgagg gcctgccggc cgttctccgc gaacgtcacc gtgtgcccga
     2641 gctgctccag cgcgcgcccg
//
LOCUS       DATMCV010000509         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_559040, whole genome shotgun sequence.
ACCESSION   DATMCV010000509 DATMCV010000000
VERSION     DATMCV010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559040"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VNU03_11745"
     CDS             complement(<1..1123)
                     /locus_tag="VNU03_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP75181.1"
                     /translation="MGQFGIGQSIKRFEDVRLLRGEGCFHADVNLPNQVHAVLVRSPH
                     AHARIRAIDGDAARRAPGVLAVFTGADVARDGLGTMKMTLKRTRPDGSPMFAPAHRGL
                     AMDRARHVGDPVALVVAATHAQAEDAAELVRVDYEPLPSVTDTGTASQPGSPPVWDEC
                     PDNVSNLHQLGDKAATDAAFAAAAHRVRRRYVITRVHAQYMEPRGALGVYDPGEDRYT
                     LYADVQYPHRVRTALATNIFKIPEHQIRVIAGDIGGAFGTKGWQYAEHRLVLWAARKV
                     GRPVKWQCERREAIPADEHARDNVSDAELALDAQGRFLGLRVQTFANVGAYVSSDRNL
                     LATFSNIATLVGVYAFPAAHVAVHSVLTNTNATAPYRGAG"
     gene            1204..>2083
                     /locus_tag="VNU03_11750"
     CDS             1204..>2083
                     /locus_tag="VNU03_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75182.1"
                     /translation="MTRRPITGVLLAVLMGLLVGVALAARSESVEVRDRSGRLGVLSI
                     TVMDDGSAYIAPDHLAALLKGAWSVKGERGILTVNQRTVEFVRGQNRATVQGQPIALD
                     AAARVVGTSWLVSRDFLGKGLPRVAPGVSIAAVAEPRKAEARPAQGAVPLEELRYRSY
                     PSFTRVVIETGARLAYAVVSSDEEVRVRLPGLSLDRSRVEEIGDGLVQEVRLESVGDA
                     AVLRVLLESAAGEIKHTVLQDPYRLVLDVYRPKESTGGGEPGRGGMQPLRLIVLDAGH
                     GGHDSGAMGPSGVMEKD"
BASE COUNT          262 a          698 c          820 g          303 t
ORIGIN      
        1 gtcccgcgcc gcggtagggg gcggtcgcgt tggtgttggt cagcaccgag tggaccgcga
       61 cgtgcgcggc cgggaaggcg tagaccccga ccagggtcgc gatgttgctg aaggtggcga
      121 gcaggttgcg gtcggacgag acgtaggcgc ctacgttggc gaaggtctgg acccgcaggc
      181 cgaggaagcg gccctgtgcg tcgagggcca gctcggcgtc gctcacgttg tcgcgcgcgt
      241 gctcgtcggc ggggatcgcc tcccggcgct cgcactgcca cttgaccggg cggccgacct
      301 tccgcgcggc ccagagcacc agccggtgct cggcgtactg ccagcccttg gtgccgaacg
      361 cgccgccgat gtcgcccgcg atgacccgga tctggtgctc cgggatcttg aagatgttgg
      421 tggcgagcgc ggtgcgcacc cggtggggat actggacgtc ggcgtagagg gtgtagcggt
      481 cctcgcccgg gtcgtacaca ccgagcgcac cgcgcggctc catgtactgg gcgtgcacgc
      541 gggtgatcac gtagcgccgg cggacccggt gggccgccgc cgcgaaggcc gcgtcggtcg
      601 ccgccttgtc gccgagctgg tggagattcg acacgttgtc ggggcactcg tcccagaccg
      661 gcgggctgcc cggctgcgac gcggtgccgg tgtcggtgac cgaggggagc ggctcgtagt
      721 ccacgcggac cagctcggcc gcgtcctcgg cctgggcgtg ggtcgcggcc accacgaggg
      781 ccaccggatc gcccacgtga cgcgcgcggt ccattgccag gccgcggtgg gccggcgcga
      841 acatggggga gccgtcgggg cgggtgcgct tgagcgtcat cttcatggtg ccgagcccgt
      901 cgcgggcgac gtcggccccg gtgaagaccg ccagcacgcc cggcgcccgc cgcgccgcgt
      961 cgccgtcgat cgcgcggatg cgggcgtggg cgtggggcga gcgcaccagc accgcgtgca
     1021 cctggttggg caggttcacg tcggcgtgga agcacccctc tccgcggagg aggcgcacgt
     1081 cctcgaagcg cttgatggac tgcccgatgc cgaattgacc catgaccgcg gagcctacca
     1141 taaattcctc ctcgccggcc cgaggctccg ttctagaatg acgatgctgc gggcgcagcg
     1201 atcatgacgc gacgaccgat cacgggtgtg ctgctggccg tgctgatggg gctcctcgtc
     1261 ggcgtcgcgc tggccgcccg ctccgaatcg gtggaggtgc gggaccggtc cgggcggctc
     1321 ggggtcctgt cgatcacggt catggacgac ggcagcgcct acatcgcacc cgatcacctg
     1381 gccgccctgt tgaagggcgc ctggtcggtg aagggcgagc gcggcatcct caccgtgaac
     1441 cagcgcaccg tcgagttcgt gcgcggccag aaccgggcca ccgtgcaggg gcagccgatc
     1501 gcgctcgacg cggccgcgcg cgtggtgggc accagctggc tcgtctcgcg cgatttcctg
     1561 ggcaagggcc tgccccgcgt cgcccccggg gtctcgatcg cggcggtcgc cgagccgcgc
     1621 aaggcggagg ccaggccggc tcagggggcg gtgccgctcg aggagctgcg ctaccgctcc
     1681 tatccctcgt tcacgcgcgt ggtcatcgag accggcgccc ggctcgccta cgcggtggtc
     1741 tcgagcgacg aggaggtgcg ggtgcggctg cccggcctct ccctcgaccg ctcgcgggtg
     1801 gaggagatcg gcgacgggct cgtgcaggag gtgcggctcg agtcggtggg cgacgccgcg
     1861 gtgctgcggg tgttgctcga gagcgcggcg ggcgagatca agcacacggt gctgcaggat
     1921 ccctaccggc tggtgctcga cgtctaccgt cccaaggagt cgaccggagg cggcgagccg
     1981 gggcgcggtg gcatgcagcc gctgcggctc atcgtgctgg acgccggcca cgggggccac
     2041 gactcgggcg cgatgggccc gagcggggtg atggagaagg acg
//
LOCUS       DATMCV010000510         5163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_294484, whole genome shotgun sequence.
ACCESSION   DATMCV010000510 DATMCV010000000
VERSION     DATMCV010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294484"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VNU03_11755"
     CDS             <1..485
                     /locus_tag="VNU03_11755"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP75183.1"
                     /translation="AEVTVRRSAEMRYFGQGHEVDVEIPTGILTEASLAPITSSFETA
                     YRLLYSRTPMGVPLEALNWRAVVSGPPPDLTITSGLETGAATAPTAKKHRAAYFPEAR
                     GYVETPVYDRYRLDPGARLAGPAIVEERESTTVIGPGALISVDAHRNLVAEPASGEAR
                     "
     gene            485..2104
                     /locus_tag="VNU03_11760"
     CDS             485..2104
                     /locus_tag="VNU03_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP75184.1"
                     /translation="MDPITLDICWSRLIGVVNEQSAALMRTSFTSIVREAGDLSAGVF
                     DRRGYMIAQAVTGTPGHINSMALAMKHILAAYPLERLTPGDVLITNDPWKTSGHLNDV
                     TVCNPIFRGRDCVAFFASTCHSADIGGHVLSAEAREVYEEGLFIPIMKLYEGGRLNES
                     LAALIRSNTRVPDLVMGDFHAQVAGGAVGGERLLEFMDEFGIERLEPLADEIITRTER
                     AMRQAIGELRPGQYRYSLTSDGFDEPITIAARCEVSGEDLWVDFTGSSPASRRGINVV
                     MNYTEAYTTYGVKVIVSPDVPNNEGAFRVLRITAPEGSILNVQHPAPVAARHIIGHFL
                     PHVVAGALKEAVPERVMAEGSANIWGIQLTGKNHDRSPWVYTFFSSGGTGARPAQDGL
                     SATAFPSGVLGTPVEVVESLAPLLIERKALRDGSGGDGRYRGGLGQVIAFRVRSDEPA
                     TCSVLCDRTRIPALGFFGGLPGLPGQVLINGRAPDNPKGEQTLRPGDLVEVLLPGGGG
                     YGPPAERDPALRERDLQQGYVVATGRGATGG"
     gene            2111..4237
                     /locus_tag="VNU03_11765"
     CDS             2111..4237
                     /locus_tag="VNU03_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75185.1"
                     /translation="MTARPRRIGVLAVAGAWWLVLAVAFLLNRGEDVGRLPALLATSL
                     AGLAHEPVWGRAGIQSLIGLLIAGLVGLAWYGLGDAIVRRVDPVEGESGDTPVMQVAT
                     RSLVGAGAWSLAWFFLGVLPLYRVSVAALALLVGLALAGRALARVRVTTAGLARCAGW
                     ASLSLIVTGQILALVAAMAPPTAKDTLLYHYALPKAWITAGRAIEIPYNIAGYYPLGV
                     EMHVVWAMLLGAPLGARVAEAAAGATLFLFAPLLAMTVYGWARERGADRTWAAAAGLV
                     IAWIPTGYDVAASAYVDLALAGYTALAVRAFGRWWTSGQPRQLGWVAAGVGGALSIKL
                     SAAFLLLPLALLGLLHALGSGSQGRETDRPSTALTAVGVLGALALGVTLAAPWYVRNW
                     IRTGSPLFPFYLEVWPAQAPGWDLERSRLYETLFSLYGDIGGPLDYLWSPIRLAVAAQ
                     PDQPAFYDGVLGIAFIFALPMLAWALWRRRLDVELRLAVLISAGLFVFWLFSSQQLRY
                     LLPAAAGLAVAIAVAGSTLERGLSRPLCGLLVAGAALGLPVVLAWFLALDPARVVLGG
                     EPREAFLARRLDYYPYYQVVNRDLPTTAMVWLIDMRRDTYHLDRAAFSDFIFEDYTLT
                     RYVRSATSVDEIRARVRADGITHLLVRHDILLDYERSPIVDERRPRQENVAKLELMAA
                     FFTQDTRVIKGDAKFWLIELPLARRR"
     gene            4436..>5163
                     /locus_tag="VNU03_11770"
     CDS             4436..>5163
                     /locus_tag="VNU03_11770"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP75186.1"
                     /translation="MGLATFLERTWSGLTGLVKPRSAAAMFRLADQYRHEGRYEEAAD
                     LIAEGLRVSPNIAVGHLLDAYVHVVFREMPAAKTSFRRVLSLDPYHPRALFGLARIAL
                     EEGDIEGCRPFLDRAIQYFPDFPEAKALQEMVATWSAPAAAVPSTVGAVNTERLRLPQ
                     GAHDLILTHADGTLVFAQGSEERQRHLSMHMTQVYRIASATLARAGLGAMRRGVIEGS
                     SEMTFVRSDGGVILGVALPREIEVG"
BASE COUNT          682 a         1877 c         1773 g          831 t
ORIGIN      
        1 acgcggaggt cacggtccgg cggagcgcgg agatgcgcta cttcggacag gggcacgagg
       61 tcgacgtgga gatcccgacg ggcatcttga ctgaagccag cctggcgccc atcacgtcct
      121 cgttcgagac ggcctatcgc ctcctttaca gccggactcc catgggggtg ccgctcgagg
      181 ccttgaactg gcgcgcggtc gtctccggcc ccccgccgga cctgaccatc acgagtgggc
      241 tcgagaccgg tgctgccacc gcgccgaccg ccaagaagca ccgggccgcg tacttcccgg
      301 aggcccgcgg ctacgtggag acgccggtgt acgaccgcta ccggctggac ccgggtgccc
      361 gcctcgcggg cccggccatc gtggaggagc gagagtcgac caccgtcatc gggcccggcg
      421 cgctcatctc ggtcgatgcc caccggaacc tggtggccga gccggcatcc ggggaggcac
      481 gctgatggac ccgatcacgc tcgacatctg ctggagccgt ctcatcgggg tggtcaacga
      541 gcagtccgcc gcgctcatgc gcacgtcgtt cacctccatc gtccgggagg cgggcgacct
      601 ctcggccggt gtcttcgacc ggcgcggcta catgatcgcc caggcggtca ccggcacccc
      661 cggccacatc aactcgatgg ccctcgccat gaagcacatc ctcgcggcct atccgctcga
      721 gcggctcacc cccggggacg tgctcatcac caacgatccg tggaagacgt ccggccatct
      781 caacgacgtc acggtatgca acccgatctt ccggggccgc gactgcgtcg ccttcttcgc
      841 gtcgacctgc cacagcgccg acatcggcgg gcacgtgctc tccgccgagg cgcgcgaggt
      901 ctacgaagaa gggctgttca tcccgatcat gaagctctac gaaggcggtc gcctcaacga
      961 gtcgctggcc gcgctgatcc gcagcaacac gcgggttccc gatctcgtga tgggcgactt
     1021 ccacgcccag gtcgcagggg gcgcggtggg tggggagcgg ctgctcgagt tcatggacga
     1081 gttcggcatc gagcgcctcg agccgctggc cgacgagatc atcacccgta ccgagcgcgc
     1141 gatgcgtcag gcgatcggcg agctacgccc gggccagtac cgctacagcc tcacctcgga
     1201 cggattcgac gagccgatca ccatcgcggc ccgctgcgag gtcagcgggg aggatctctg
     1261 ggtcgacttc accggctcct cgccggccag ccgccgcggc atcaacgtgg tcatgaacta
     1321 caccgaggcc tacacgacct acggggtcaa ggtcatcgtg agccccgacg tccccaacaa
     1381 cgagggcgcc ttccgcgtgc tccgcatcac cgcgcccgag ggctccatcc tgaacgtgca
     1441 gcaccccgcc ccggtggcgg cccgtcacat catcggacac ttcctgccgc acgtggtcgc
     1501 gggagccctg aaggaggcgg tgccggagcg ggtcatggcc gagggctcgg ccaacatctg
     1561 gggcatccag ctcaccggca agaaccacga ccgctcgccc tgggtctaca cgttcttcag
     1621 ctccggcggc accggggcgc ggccggctca ggacggcctg tcggccaccg cctttccctc
     1681 gggagtgctc ggcaccccgg tggaggtggt cgagtcgctc gccccgctgc tgatcgagcg
     1741 caaggcgctg cgggacggat ccggcggcga cgggcgctac cggggcgggc tcgggcaggt
     1801 gatcgccttc cgggtgcgct ccgacgagcc ggccacgtgc tcggtcctgt gcgaccgcac
     1861 tcgcattccg gccttgggat tcttcggtgg gctgccgggc cttccgggcc aggtcctgat
     1921 caatgggcgt gctcccgaca acccgaaggg ggagcagacg ctccgacctg gagatctggt
     1981 cgaggtgctc cttccgggag gcggcggata cggtcctccc gcggagcgcg acccggcgct
     2041 ccgggagcgc gacctccagc agggctacgt cgtagcgacc ggccgaggcg ccaccggcgg
     2101 ctgaccgggc atgacggctc ggccccggcg gatcggcgtc ctcgccgtcg cgggcgcctg
     2161 gtggctcgtg ctcgcggtgg ccttcctgct gaaccgcggc gaggacgtgg ggcggctccc
     2221 cgccctgctg gcaaccagtc tcgccggtct cgctcacgag ccggtgtggg gccgcgcggg
     2281 gatccagtct ctgatcggtc tactgatcgc cggcctcgtc ggcctcgcat ggtatggcct
     2341 cggcgacgcg atcgtccgcc gcgtcgaccc cgtggagggt gagtctggcg ataccccggt
     2401 gatgcaggtt gccacgcgct cgctggtcgg tgccggtgcc tggtcgctgg cctggttctt
     2461 cctgggggtc ctgcccctct accgggtctc ggtcgcggcg ctggcgctcc tggtgggcct
     2521 ggccctggcc ggccgggcgc tcgcacgcgt ccgggtcacc acggccggcc tcgcgcgctg
     2581 cgccggctgg gcttcgttga gtctgatcgt caccggccag atcctggcgc tggtcgccgc
     2641 catggcgccg ccgacggcaa aagataccct cctctaccac tacgcgctcc ccaaggcctg
     2701 gatcaccgcg ggtcgcgcga tcgagatccc ctacaacatc gccggctact acccgctggg
     2761 cgtcgagatg catgtggtct gggcgatgct cctgggcgcg ccgctcggcg ctcgcgtcgc
     2821 cgaggccgcg gccggcgcca ccctctttct cttcgcgccg ctcctcgcga tgacggtgta
     2881 cgggtgggcg cgagagcgag gggcagatcg gacgtgggcg gccgccgctg gcctcgtgat
     2941 cgcctggatc cccaccgggt acgacgtggc cgcgagcgcc tacgtggacc tggccctggc
     3001 cggttacacc gcgctggcgg tgcgggcgtt cggacgctgg tggacgagcg ggcaaccgcg
     3061 tcagctcggc tgggtcgcgg ccggtgtcgg gggcgccctc tccatcaagc tcagcgccgc
     3121 gttcctgctg ctccccctcg cgctgctggg gctgctgcac gccctcggct ccggaagcca
     3181 gggccgcgag accgaccggc ccagcaccgc cctgacggcc gtcggagtcc tgggagccct
     3241 ggcccttggc gtgaccctcg ccgcgccctg gtacgtgcgg aactggatcc gcaccgggag
     3301 cccgctcttc ccgttctatc tcgaggtctg gcccgcccag gcccccggct gggacctcga
     3361 gcggtcacgt ctgtacgaga ccttgttctc gctgtacggc gacatcggcg gccccctcga
     3421 ctacctctgg tcgccgatcc gtctcgcggt ggccgcccag ccggatcagc ccgccttcta
     3481 cgacggcgtg ctcggtatcg ccttcatctt cgcgctgccc atgctcgcgt gggccctgtg
     3541 gcggcgccgc ctcgacgtcg agttgcgcct ggccgttctc atctcggccg gcctcttcgt
     3601 cttctggctc ttctccagcc agcagctccg gtacctgttg cccgccgcgg cgggcctggc
     3661 ggtggccatc gcggtggccg gcagcaccct cgagcgcggg ctgagccggc cgctgtgcgg
     3721 cctcctggtg gccggcgccg ccctcggcct gccggtcgtg ctggcctggt tcctggcgct
     3781 ggatccggcg cgggtcgtgc tcgggggcga gccccgcgag gcctttcttg cccggcgact
     3841 cgactactat ccctactacc aggtcgtgaa ccgagacctt cccacgaccg ccatggtctg
     3901 gctcatcgat atgcgccggg acacctacca cctcgatcgg gcggccttct ccgatttcat
     3961 cttcgaggac tacacgctca cccggtacgt gcgctcggcg accagcgtcg acgagatccg
     4021 ggcccgggtc cgggccgacg gcatcacgca tctgctggtg cggcacgaca tcctgctcga
     4081 ctacgagcgc tcacccatcg tggacgagcg gcgcccgcgc caggagaacg tcgcgaagct
     4141 cgagctgatg gccgcgttct tcacgcagga cacccgcgtg atcaagggcg acgcaaagtt
     4201 ctggctcatc gagctgcccc tggcccggcg ccgctgagcg ggctcgcggc gcgagccgcc
     4261 gcccgagacc gccaggcccc ggagccagga aatgtcttcc cggcctgcgg gtggggcccc
     4321 gaccacgatc tcgatagcgc ccggtagttt ggaagcgcct cggggcctgc gtgctatgat
     4381 ggccaacaac gatgatctcc aggctgggca acccctggac gagggaggtc ggcgcgtggg
     4441 tctagccacg ttcctcgagc gtacctggag cggtctcacc gggctcgtca agccgcggtc
     4501 cgcggcggcg atgttccgtc tggccgacca gtaccggcac gagggccgct acgaagaagc
     4561 ggccgacctg atcgccgagg gcctgcgcgt gtcgcccaat atcgcggtcg ggcacctgct
     4621 ggacgcgtac gtccacgtgg tgttccgcga gatgccggcg gccaagacct ccttccggcg
     4681 ggtgctctcc ctcgatccgt atcacccgcg ggccttgttc gggctcgccc gcatcgccct
     4741 ggaagagggc gacatcgagg ggtgccggcc ctttctggat cgcgcgatcc agtacttccc
     4801 cgatttcccg gaagcgaagg cgctccagga gatggtcgcg acctggtcgg cgccggccgc
     4861 ggcggtaccg agcacggtcg gggccgtcaa caccgagcgg ctccgtctac cgcagggcgc
     4921 gcacgatctc attctcaccc acgccgacgg gacgctggtg ttcgcgcagg gcagcgagga
     4981 gcggcagcgg cacctgtcca tgcacatgac ccaggtctat cgaatcgcct ccgccactct
     5041 cgcccgagcg ggcctgggag cgatgcgtcg aggggtcatc gagggctcat cggagatgac
     5101 attcgtgaga agcgacggcg gcgtgatcct aggggtcgcg ctccctcgcg agatcgaggt
     5161 cgg
//
LOCUS       DATMCV010000511         3330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_895472, whole genome shotgun sequence.
ACCESSION   DATMCV010000511 DATMCV010000000
VERSION     DATMCV010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895472"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VNU03_11775"
     CDS             complement(<1..338)
                     /locus_tag="VNU03_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWP75187.1"
                     /translation="MGLSLTRKLIDAHLVAGKAVAGDEIGLRVDQALLTDTNGTMAFL
                     QFEAMGFPRVRPPRVVAYIDHNVYQVDSRNSDDHRYLQTASSRYGAWFSKPGNGICHQ
                     VHFESFSVPG"
     gene            complement(349..1479)
                     /locus_tag="VNU03_11780"
     CDS             complement(349..1479)
                     /locus_tag="VNU03_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWP75188.1"
                     /translation="MTESSQLIRGLEGVVAAETRLCDLDGKNGRLAYGGYDIDELARK
                     ATFEEVAYLLWHGELPARVQLDRFQVELAAVRTIPDDLVRAYALMPRDTDPMRVLQAS
                     VAVLGMHDPDATDSSRPANLRKAVRLTSQFATAICAHHRVRSGQAPVPPDPGLSLAAN
                     FLYMLTGARPTAVTTKAFDACLTLYAEHELNASTFTTRVIAATLSDMHSAVAGGVGAL
                     KGTLHGGAGEAVMRTLLEVGRLDNVDAFVDRSMGEKRRLMGFGHRVYTAGDPRAAILK
                     GMAEEACRQSGQALWYDLAVKLHAKVQATKRLIPNVDFYSAPLFYSIGIPVDLFTPVI
                     ATSRIAGWTANLLEQYDDNRLIRPRADYKGPARRAFVAVDKR"
     gene            1632..>3330
                     /locus_tag="VNU03_11785"
     CDS             1632..>3330
                     /locus_tag="VNU03_11785"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HWP75189.1"
                     /translation="MALPVASGRSAVTAGAVGPRPSLLDSLRRAWPAYVVLGCTLLLT
                     FAAWRYARHTVRADEQERFARVVAVSREAIDRRFDSYVQILLGVRALFAGSAEVERGE
                     FRSYVAALGLEGRYEGIRGIGWAPRVPSALRARHEAAIRRQGLPAYEIHPAESGPEAF
                     PIVFLEPAGFEERALGLDALSRAGNRGALERARDTGEPAMSERLMLLRADGGLGFVIF
                     VPVYRGGGVPGTVEERRRDLEGFVSASFRPDLMLRGIFGSSLGEPVDLEVYDGPDPRP
                     ERLLYDRDPSAGPADVPRELGHWLALTFGGHTWTLHFSTLPAFHSRPDRLIPPAILGS
                     GLLISVLAFVITLLQAHALLRARRLGSEIQAADRRLRQANERFEMAASAVKSAIYDWS
                     LRHGTVVWTSGLTEMCGYPLDEVSGTYEWRVDRLHPDDRARVHGQVETAAAEGRDFET
                     EYRFRTRDGGYLEVQDRGRFVRDGAGRAVRMVGSMVDISERKRAEAVLRESEARHRAV
                     IESAMDAFVSMDHEGRVTEFNPAAERMFGLARADVLGQELAAVIVPPAYRDAHRQGLT
                     "
BASE COUNT          449 a         1144 c         1249 g          488 t
ORIGIN      
        1 tccccgggga cgctgaacga ctcgaagtgc acctgatggc agatgccgtt gccgggcttg
       61 gagaaccacg cgccgtagcg gctcgacgcg gtctggaggt agcggtggtc gtcggagttg
      121 cgcgagtcca cctggtagac gttgtggtcg atgtaggcga ccacgcgcgg cggccgcacc
      181 cgcgggaagc ccatcgcctc gaactggagg aacgccatgg tgccgttggt gtcggtgagc
      241 agggcttgat cgacgcgcag gccgatctcg tccccggcca ccgccttccc ggcgaccaga
      301 tgggcgtcga tgagcttgcg ggtgagactc aggcccatgg gcgcgggcct aacgcttgtc
      361 gaccgcgacg aaggcgcggc gggcggggcc cttgtagtcg gcgcggggtc ggatcaggcg
      421 gttgtcgtcg tactgctcga gcaggttggc ggtccagccc gcgatgcggg aggtggcgat
      481 gaccggggtg aagagatcca ccgggatgcc gatcgagtag aagagcggcg cggagtagaa
      541 gtccacgttc gggatcagtc gcttggtggc ctgcaccttg gcgtggagct tgaccgcgag
      601 gtcgtaccag agggcctggc ccgattgccg gcaagcctcc tcggccatgc ccttcaggat
      661 cgcggcgcgc ggatcgcccg cggtgtagac ccggtggccg aagcccatca gtcgccgctt
      721 ctcgcccatc gaccggtcga cgaaggcgtc gacgttgtcg aggcggccga cctcgagcag
      781 ggtgcgcatc accgcctcgc cggccccgcc gtgcagggtg cccttgagcg cgcccacccc
      841 gcccgcgacc gccgagtgca tgtcggagag cgtcgccgcg atcacgcgcg tggtgaaggt
      901 cgaggcattc agctcgtgct cggcgtagag ggtgaggcac gcgtcgaagg ccttggtggt
      961 caccgcggtg ggccgggcgc cggtgagcat gtagaggaag ttggccgcga gcgagaggcc
     1021 ggggtccggc ggcaccggag cctggccgct ccgcacccgg tggtgcgcgc agatcgcggt
     1081 ggcgaactgg ctggtgagcc gcaccgcctt gcgcaggttg gcgggccggg agctgtcggt
     1141 cgcgtcgggg tcgtgcatgc cgagcaccgc gaccgacgcc tgcagcacgc gcatcgggtc
     1201 cgtgtcgcgg ggcatcaggg cgtaggcgcg aaccagatcg tccgggatcg tgcgcaccgc
     1261 ggccagctcc acctggaagc ggtcgagctg cacccgcgcg ggcagctcgc cgtgccacag
     1321 caggtaggcg acctcctcga aggtggcctt ccgcgcgagc tcgtcgatgt cgtatccgcc
     1381 gtaggccagc cggccgttct tgccgtccag atcgcacaga cgcgtctcgg cggcgaccac
     1441 gccctcgaga ccccggatga gctggctgct ctcggtcatc gtcgactccc ctgggcactc
     1501 cggatgctcc gcggacggca gacgcgggcg aacatgacga agtcgacggt ataccgcgtc
     1561 ccggaagtgt gcaagagcat tggagacgga cgccggaccg agtagaatcg acccggaccc
     1621 tccgagccat catggcgctg ccagtcgcat cgggccgctc agcagtcacg gccggcgccg
     1681 tcgggccgcg gccgtccctg ctcgactccc tgcgccgcgc ctggcccgcc tacgtcgtcc
     1741 tcggctgcac gctgctcctg acgttcgcgg cctggcgcta cgcccggcac accgtgcgag
     1801 ccgacgagca ggagcgcttc gcccgggtgg tcgcggtgag ccgggaggcg atcgaccggc
     1861 gcttcgattc ctacgtgcag atcctcctgg gggtacgcgc gctcttcgcc ggcagcgccg
     1921 aggtggagcg aggcgagttc agatcctacg tcgcggcgct ggggctcgag gggcgctacg
     1981 agggcattcg cgggatcggc tgggccccgc gggtgccctc cgcgctccgg gcccgtcacg
     2041 aggcggcgat ccggcggcag gggctacccg cgtacgagat ccacccggcc gagagcgggc
     2101 cggaggcctt cccgatcgtc ttcctcgagc cggccggctt cgaggagcgg gcgctcggcc
     2161 tcgacgcgct gagccgggcc ggcaaccgcg gagccctcga gcgggcccgc gataccggag
     2221 agcccgcgat gagcgagcgg ctgatgctcc tgcgggcgga cggcgggctc ggcttcgtca
     2281 tcttcgtccc ggtgtatcgc gggggcgggg tgccgggcac ggtcgaggag cggcgccgcg
     2341 atctggaagg gttcgtgtcc gcgtcgttcc gtcccgacct catgctccgc ggcatcttcg
     2401 gctcctcgct gggggagccg gtggacctcg aggtctacga cggccccgac ccgcggccgg
     2461 agcgcctgct ttacgaccgc gacccgagcg cggggccggc cgacgtcccg cgggagctcg
     2521 gccactggct cgcgctgacc ttcggcggcc acacctggac gctccacttc tccacgctgc
     2581 ccgccttcca ctcccgcccg gatcggctca tcccgcccgc gattctcggc agcggactcc
     2641 tgatcagcgt gctcgccttc gtgatcaccc tgctccaggc ccacgcgctg ctgcgcgcgc
     2701 gccggctcgg cagcgagatc caggccgcgg accgccgcct ccgtcaggcc aacgagcgct
     2761 tcgagatggc cgcctccgcg gtgaagtccg cgatctacga ctggagcctc cgccacggca
     2821 ccgtggtgtg gacctcgggg ctcaccgaga tgtgcggcta cccgctcgac gaggtgagcg
     2881 ggacctacga gtggcgggtc gatcgccttc atccggacga tcgggcgcgg gtgcacgggc
     2941 aggtcgagac cgccgcggcg gagggccgcg acttcgagac cgagtaccga ttccggaccc
     3001 gcgacggcgg ctacctggag gtgcaggatc gcgggcggtt cgtgcgggac ggcgcgggac
     3061 gcgcggtgcg catggtcggc tccatggtcg acatctccga gcgcaagcgc gcggaggcag
     3121 ttctccggga gagcgaggcg cggcatcgcg cggtgatcga gtcggcgatg gacgccttcg
     3181 tgagcatgga ccacgagggc cgggtgaccg agttcaatcc ggccgccgag cgcatgttcg
     3241 gccttgcgcg ggcggacgtc ctgggccagg agctcgccgc ggtgatcgtg ccgcccgcgt
     3301 accgggacgc gcaccgacag gggctgaccc
//
LOCUS       DATMCV010000512         5239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_162926, whole genome shotgun sequence.
ACCESSION   DATMCV010000512 DATMCV010000000
VERSION     DATMCV010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162926"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /gene="rpsB"
                     /locus_tag="VNU03_11790"
     CDS             complement(<1..770)
                     /gene="rpsB"
                     /locus_tag="VNU03_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWP75190.1"
                     /translation="MASLTMKELLEAGVHFGHQTKRWNPKMQKYIFGERNGIYIIDLQ
                     KTLKKFREAYAFVRDLAADGGTVLFVGTKKQAQETVLEETARCGQFYVNHRWLGGTLT
                     NFTTIRKSIARLKKLDEMSETGEYERLPKKEVIGLERERAKLQSALVGIKSMDRLPSA
                     IFIIDPKKESIAVEEARRLAIPIVAIVDTNCDPSGIDYPVPGNDDAIRSVRLITSRIA
                     DAIIEGSGTLAKQEVEEIPASPDLPLVTEAEMSATAEPG"
     gene            complement(900..2114)
                     /gene="argJ"
                     /locus_tag="VNU03_11795"
     CDS             complement(900..2114)
                     /gene="argJ"
                     /locus_tag="VNU03_11795"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWP75191.1"
                     /translation="MADFQWLEGGITAVPGILASGIAAGIKPSGKKDLALVYSSSPAR
                     AAAVFTTNQIKGAPVLVSQEHVRDGRAQAILASAGCSNVCTGEQGVKDAREMTRITGE
                     LLRIAPRQVLIASTGVIGQPLPMDKIRAGLPKLVKGLSPQGGRLAAEAIMTTDTRPKE
                     AALRVDVAGRPVTIGGIAKGVGMIEPHMATMFCFLATDAMVARDSLPRVLRRSVERSF
                     NRITVDGDQSTSDTVALLANGLAENASLERGGKGLRQFARALEAITERLARMLVEDGE
                     GATKLVEIAVRGARTRREAVLAARSVANSPLVKTAINGADPNWGRIMMALGKGPARVA
                     PDRVAIAIGGELLVEKGMLRAGARLDRIHEMMGGASYTIAIDLGLGRGEDAVWTSDLS
                     EEYVRINAKYTT"
     gene            complement(2120..3169)
                     /gene="argC"
                     /locus_tag="VNU03_11800"
     CDS             complement(2120..3169)
                     /gene="argC"
                     /locus_tag="VNU03_11800"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWP75192.1"
                     /translation="MLKVAVTGASGYMGAELLRLLSVHPKVRIAAVTSERLAGERLDK
                     SYPHLRGLSDLVFQDIDPERLAAESDLVFLALPHMESQRLMPVLRRSGRKAIDLSADY
                     RLKDAALYTTWYKAPHEDAAGLAEAVYGLPELHRKQIAAASLIASPGCYPMGAVLATA
                     PLLKSGRGAVDGIVIDGKSGVTGAGAQGRKAEPMYLFTEANENVQAYGMATHRHTPEI
                     EQELSALAGQPVVVSFTPHLVPLNRGLFTTASVPLTRPATTAELVALYREFYAGEPFV
                     RVLDEGQRPTTRSVVGSNYCDVTVVADPRTKRAVCVSALDNLGKGGSANGIQSLNIMM
                     GWDERTGLEAPPVYP"
     gene            complement(3189..3584)
                     /gene="rpsI"
                     /locus_tag="VNU03_11805"
     CDS             complement(3189..3584)
                     /gene="rpsI"
                     /locus_tag="VNU03_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWP75193.1"
                     /translation="MAVVTRFYGTGRRKTSVARVWLRAGNGRILVNRRPFEDYFPRET
                     LRMVISQPLQLTNTLGQFDAMVNVGGGGPTGQAGAVRHGIARALLQFDDKLRQTLKRA
                     GLLTRDPRMKERKKYGQPGARSKFQYSKR"
     gene            complement(3593..4036)
                     /gene="rplM"
                     /locus_tag="VNU03_11810"
     CDS             complement(3593..4036)
                     /gene="rplM"
                     /locus_tag="VNU03_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWP75194.1"
                     /translation="MTTLMPKEEEVERKWFLVDASDRVLGRLATQVATVLRGKHKPQF
                     SPHLDVGDHVVVINAEKIHLTGRKLTDKIYRWHSGYIGGLREVSAERMIKTHPERVIE
                     WAVQGMLPKGRLGRAMAKKLKVYKGAEHPHAAQKPEPLATRARAR"
     gene            complement(4113..4817)
                     /gene="nth"
                     /locus_tag="VNU03_11815"
     CDS             complement(4113..4817)
                     /gene="nth"
                     /locus_tag="VNU03_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWP75195.1"
                     /translation="MAESLPAKKARARKIMSRLARVYPDARTELRFDTPLDLLVATIL
                     SAQCTDERVNAVTAELFPRYRRAQDWAGVPLPDLERLIHSTGFFRAKARSISGMAKAL
                     VERHGGEVPRTLDELVALPGVGRKTANVVLGNAFGIPALAVDTHVFRVSQRLGLARGD
                     DPEKIHDQLCLVLPQPSWTQASHLLILHGRRVCFARKPACPTCPVEALCPFPGKTKAA
                     KPVKAAARPRPRRSGS"
     gene            complement(4817..5161)
                     /locus_tag="VNU03_11820"
     CDS             complement(4817..5161)
                     /locus_tag="VNU03_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75196.1"
                     /translation="MTGRATGALAALLLGLAAVMAVWLSIDRRPPEWDHANHRAGPAP
                     APFTLHVDGVSYGGALLPGLLVRWIVRNFDPTPRLRRLPMGVSLAPISFGAGRVEIGR
                     DPDPSCPPKESR"
BASE COUNT          718 a         1834 c         1861 g          826 t
ORIGIN      
        1 cccggctccg ccgtcgccga catctcggcc tcggtcacca gcggcaggtc cggcgaagcc
       61 gggatctcct ccacctcctg cttggcgagg gtgccgctgc cctcgatgat cgcgtccgcg
      121 atgcgcgagg tgatcaggcg caccgagcgg atggcgtcgt cgttgccggg caccgggtag
      181 tcgatcccgg aggggtcgca gttggtatcc acgatggcca cgatcgggat cgcgagccgc
      241 cgcgcctcct ccaccgcgat cgactccttc ttggggtcga tgatgaagat cgcggagggc
      301 agccggtcca tgctcttgat gcccaccagc gcgctctgga gcttggcccg ctcgcgctcg
      361 agcccgatga cttccttctt gggcaggcgc tcgtactcgc cggtctcgct catctcgtcg
      421 agcttcttga gccgcgcgat cgacttgcga atcgtcgtga agttggtgag ggtgccgccc
      481 agccagcggt ggttgacgta gaactgcccg cagcgggccg tctcctccag caccgtctcc
      541 tgggcctgct tcttggtgcc cacgaacagc acggtgccgc cgtcggcggc gaggtcgcgc
      601 acgaaggcgt aggcctcgcg gaacttcttg agggtcttct gcagatcgat gatgtagatg
      661 ccgttgcgct cgccgaagat gtacttctgc atcttcggat tccagcgctt ggtctggtgg
      721 ccgaagtgca ctccggcctc cagcagctcc ttcatggtca gtgatgccat aatccggcgt
      781 tcctttccgt cgtcgttcgg ttggcctccg gtcgcgagcg gccggccccc ggaaaggggg
      841 gcgacacttc cccgggggcc gacgcggccc gcggagaccg aagctcgcaa ccgtgcgtgt
      901 taggtcgtgt acttcgcgtt gattcgaacg tactcctcac tgaggtccga tgtccacacc
      961 gcgtcctcgc cccgtcccag tcccaggtcg atggcgatgg tgtagctcgc cccacccatc
     1021 atctcgtgaa tccggtcgag acgggctccc gcccgcagca tccccttctc cacgagcagc
     1081 tcgcccccga tcgcgatggc cacccggtcc ggggccaccc gggccgggcc cttgcccagc
     1141 gccatcatga tgcggcccca gttggggtcg gcgccgttga tcgcggtctt gacgagcggc
     1201 gagttggcca ccgagcgagc ggccagcacc gcctcccgcc gggtgcgggc gccgcgcacc
     1261 gcgatctcca ccagcttcgt cgcgccctct ccgtcctcga cgagcatgcg ggcgaggcgc
     1321 tcggtgatcg cctcgagcgc gcgcgcgaac tggcgcagcc ccttgccccc gcgctcgagc
     1381 gacgcgttct cggccagccc gttggcgagc agggccaccg tgtcgctggt ggactggtcg
     1441 ccgtccaccg tgatgcggtt gaacgagcgc tcgaccgagc ggcgcagcac gcggggcagc
     1501 gagtcgcgcg ccaccatcgc gtcggtggcg aggaagcaga acatcgtggc catgtgcggc
     1561 tcgatcatgc cgacgccctt ggcgatgccc ccgatggtca ccggccggcc cgccacgtcg
     1621 acccgcaggg ccgcttcctt cggccgcgtg tcggtggtca tgatggcctc cgccgcgagc
     1681 cgcccaccct ggggcgagag gcccttcacc agcttgggca gcccggcccg gatcttgtcc
     1741 atcggcagcg gctggccgat gacgccggtg gaggcgatca ggacctggcg cggcgcgatg
     1801 cgcagcagct ccccggtgat ccgggtcatc tcgcgcgcgt ccttcactcc ctgctcaccg
     1861 gtgcagacgt tcgagcaccc cgcggaggcc aggatggcct gggcccggcc gtcgcgcacg
     1921 tgctcctgcg acaccagcac cggcgcgccc ttgatctggt tggtcgtgaa caccgcggcc
     1981 gcgcgcgcgg gcgacgagga gtagacgagg gccaggtctt tcttgccgct gggcttgatg
     2041 ccggccgcga tgccggaggc gaggatgccg ggcacggcgg tgatgccccc ctccagccac
     2101 tggaagtccg ccatgcgggc tacggataca ccggaggcgc ttcgagaccg gtccgctcgt
     2161 cccaccccat catgatgttg aggctctgga tgccgttggc ggagccgccc ttgcccaggt
     2221 tgtcgagcgc cgacacgcac accgcgcgct tggtgcgcgg gtcggccacc accgtgacgt
     2281 cgcagtagtt cgagcccacc accgaccggg tggtcgggcg ctggccctcg tcgagcaccc
     2341 gcacgaacgg ctcgcccgcg tagaattccc ggtacagcgc gaccagctcc gcggtggtgg
     2401 ccggccgggt gagcgggacg gacgcggtgg tgaacaggcc ccggttgagg gggacgaggt
     2461 gaggcgtgaa gctcacgacc accggctgcc ccgcgagggc ggagagctcc tgctcgatct
     2521 ccggggtgtg gcgatgggtg gccatgccgt aggcctgcac gttctcgttg gcctcggtga
     2581 agaggtacat cggctccgcc ttgcgcccct gcgctccggc gccggtgacg cccgacttgc
     2641 cgtcgatcac gatcccgtcg accgcgcccc gcccgctctt cagcagcggc gcggtcgcga
     2701 gcaccgcgcc catcgggtag cagcccggcg aggcgatgag cgacgcggcc gcgatctgct
     2761 tgcggtgcag ctcgggcagc ccgtagaccg cctccgcgag ccccgccgcg tcctcgtgcg
     2821 gggccttgta ccaggtggtg tagagcgcgg cgtccttcag gcgatagtcc gccgagaggt
     2881 cgatcgcctt ccggccgctc cggcgcagca ccggcatgag ccgctgcgac tccatgtggg
     2941 gcagcgcgag gaagaccagg tccgactccg ccgcgagccg ctcggggtcg atgtcctgga
     3001 agacgaggtc gctcagcccg cgcaggtgcg ggtagctctt gtcgaggcgc tcgcccgcga
     3061 ggcgctccga ggtgaccgcg gcgatgcgca ccttcggatg cacggagagg agacggagca
     3121 gctccgcgcc catgtacccg ctggctccgg tgaccgcgac tttcagcatg gccggatccg
     3181 cgggcgggct accgcttcga gtactggaac ttcgaccggg cgccgggctg gccgtacttc
     3241 ttgcgctcct tcatccgcgg atcgcgggtg aggaggccgg cccgcttgag cgtctggcgc
     3301 agcttgtcgt cgaactgcag cagggcccgc gcgatgccgt ggcggaccgc gcccgcctga
     3361 cccgtcggcc caccgccgcc cacgttcacc atcgcgtcga actggcccag ggtgttcgtg
     3421 agctggagcg gctgggagat gaccatccgc agcgtctcgc gggggaagta gtcctcgaac
     3481 gggcggcggt tgaccaggat gcggccgttg ccggcccgca gccagacacg cgccaccgag
     3541 gtcttgcggc gtccggttcc atagaaacga gtcacgacgg ccatgggact cctcagcggg
     3601 cgcgcgcgcg cgtggcgagc ggctcgggct tctgggcggc gtggggatgc tccgcccctt
     3661 tgtagacctt cagcttcttg gccatcgcgc ggcccaggcg gcccttgggg agcatgccct
     3721 gcaccgccca ctcgatcacg cgctcgggat gggtcttgat catccgctcc gcgctcacct
     3781 cgcgcaggcc tccgatatag ccgctgtgcc agcggtagat cttgtcggtc agcttgcgtc
     3841 cggtcaggtg gatcttctcc gcgttgatga ccaccacgtg gtcgcccacg tcgaggtgcg
     3901 gggagaactg gggcttgtgc ttgccccgga ggaccgtcgc gacctgggtg gccagacggc
     3961 ccagcacccg gtcactggcg tcgaccagga accacttgcg ctcgacttcc tcttccttcg
     4021 gcatcagcgt cgtcatgatc tcgtccggag ccccccgttg aatggcaacg ggtaatcata
     4081 aaggaacggc tcgaccgaat caactgccag ggtcaactgc cagaccgtcg cggccgggga
     4141 cgggccgcgg ccttgaccgg cttggccgcc ttggtcttgc ccgggaaggg gcagagggcc
     4201 tccaccgggc aggtggggca ggccggcttc cgggcgaagc agacccgccg gccgtggagg
     4261 atgagcaggt ggctcgcctg ggtccagctc ggctggggca gcaccaggca gagctggtcg
     4321 tggatcttct cggggtcgtc cccgcgggcc aggcccaggc gctgcgagac ccggaacacg
     4381 tgggtgtcga ccgccagggc cgggatgccg aaggcgttgc cgagcaccac attggcggtc
     4441 ttccgcccca ccccgggcag cgcgacgagc tcgtcgaggg tgcgaggcac ctcgccgccg
     4501 tgacgctcga ccagggcctt ggccatcccc gagatcgagc gggccttggc ccggaagaac
     4561 ccggtcgagt ggatcaggcg ctcgaggtcc gggagcggca cgcccgccca gtcctgcgcg
     4621 cggcggtagc gcgggaacag ctccgcggtg accgcgttga cgcgctcgtc ggtgcactgc
     4681 gcggacagga tggtggccac cagcagatcg agcggggtgt cgaacctgag ctcggtgcgc
     4741 gcgtccgggt acacgcgggc gaggcggctc atgatcttgc gggcgcgcgc cttcttggcg
     4801 ggcaggctct cggccatcag cgtgactcct ttggcgggca ggacggatcc ggatctcgac
     4861 cgatctcgac ccgccctgcc ccgaaggaga tcggagcgag cgacaccccc atgggcagtc
     4921 gccgcaggcg cggggtcggg tcgaagtttc tcacgatcca gcgcacgagg agaccgggca
     4981 gaagcgcgcc cccgtagctg accccgtcga cgtgcagggt gaacggcgcg ggcgcaggcc
     5041 ccgcgcggtg gttggcgtgg tcccactccg gcgggcgtcg atcgatggag agccagaccg
     5101 ccatgaccgc ggccagcccc aggagcagcg cggccagagc cccggtggcg cgtccggtca
     5161 tcggcccggc gcgggctcgg cggcgcgggc gaccagcaag atcgagatgc cgaagggcag
     5221 ggagacgaag cgcaggagg
//
LOCUS       DATMCV010000513         3519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_95256, whole genome shotgun sequence.
ACCESSION   DATMCV010000513 DATMCV010000000
VERSION     DATMCV010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95256"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VNU03_11825"
     CDS             complement(<1..662)
                     /locus_tag="VNU03_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75197.1"
                     /translation="MLVRAIGLGKTFRGPDGGPIPVLDGVDVEVKPGEFVAVAGPSGS
                     GKSTLLNLLGLLEAVDRGEIWLDDVCVSRLSRRAQCPVRGRSIGYVFQSFLLIAGLTA
                     LDNVLLAARYVGRSRADAQPAALALMERLGVAHRRGHYPSQLSGGEQQRVAYCRAVLN
                     APPLVLADEPTGNLDEGHAAVILSELRGLCRERGASVILVTHRADAAALADRTLRLRA
                     GRL"
     gene            complement(674..1867)
                     /locus_tag="VNU03_11830"
     CDS             complement(674..1867)
                     /locus_tag="VNU03_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75198.1"
                     /translation="MVGIARLLPKNYLFTILGIAVGVSGLVALGAMAERITRFIDGGD
                     RFVLGQISVAGEGMGMGTGFTAGGLLRAAKIEQIAQVPGVAAVQAQVMLPLNPTTSQF
                     LTLTQELVLGIDPATPMPNRQFRELPVKSGRFLRANDRGVAVVGADFAATRKLAVGGR
                     LELAAGRTFEVVGVLDKTLTAPDRFAIIPLDDARDLWLRRDPLLVQVFAGGAGMGRRD
                     LNTGAAVAWADGVDADDLARRIQAGVPGVNVTIPGELSRLLRQSTAFFSALLFGIGVL
                     GLVIGGLSLSNTVTAAVFERIRDFGIKRALGATDLQLLREILGEALGVSISGGALGVG
                     LALAIGTLVDARVLRDGQQLFLFSPRLLVFAIAFSVILGGLAATYATLRIVRLSPAEA
                     IRRGT"
     gene            complement(1861..>3519)
                     /locus_tag="VNU03_11835"
     CDS             complement(1861..>3519)
                     /locus_tag="VNU03_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HWP75199.1"
                     /translation="WAREAAAGAAWAVGIFSPARLRARHRDMYRFAAPHRSHPPGWAL
                     AVGAFVADRALRTVERGLGSDHARGLRMAAVATAATPLLLVLALGRSALAALGIDWLG
                     PVVRNLYPQGAMAATLEAPARRAAGAARPADYDVLVVGGGPGGSSAATFLSRGGLRVA
                     LAEREAFPRFHVGESLLPANMPVLERLGVLDAVKARGFLVKYGAYFHDQDMDLGYQFF
                     FREGKPWPSYTYEVQRGEFDKILLDHAARQPNVTLLQPATVERVAFDDAGVTATVAEG
                     AGSREIRARFLVDASGRDAFLATRHGRRRPIEGLGKVALFAHFQGGKRWPGKEAGNIR
                     IFSFEPGWFWYIPFHNGTSSVGCVLHARTVRGREGNLEQLYESLIARCRGLVDALDGA
                     PRITPVHSAANFSYQTEPAVGDRFVCVGDAIAFLDPIFSSGVYLATQSAELSSAEILK
                     AFRENRFAARRFRAYQKAIRRGMQPFQRFIRNFYDPSFTEVFLNPREFAGMVDGVTGV
                     LAGGAFLRMSLRMRLSLEVFFTVVRVNRWVRRRRGLATESRFKW"
BASE COUNT          554 a         1270 c         1300 g          395 t
ORIGIN      
        1 agccgccccg cacgcaggcg cagggtgcgg tccgcgaggg cggccgcgtc ggcccggtgc
       61 gtgacaagga taaccgaggc cccccgctca cgacacaggc cccggagctc ggacaggatc
      121 accgcggcgt ggccctcgtc caggttcccg gtcggctcgt cggccagcac cagcggcggg
      181 gcgttgagca ccgcccggca gtacgccacc cgctgctgct ccccgcccga aagctgcgag
      241 ggatagtggc cgcgccggtg agcgactccg agccgctcca tcagcgcgag cgcggcgggc
      301 tgcgcgtcgg cgcgcgaccg cccgacgtag cgggcggcca gcagcacgtt gtcgagcgcg
      361 gtgagacccg cgatcagcag gaacgactgg aagacgtagc cgatcgagcg gccgcgcacc
      421 gggcactggg ctcggcgcga gagccggctc acgcacacgt cgtcgagcca gatctcgccg
      481 cggtccaccg cctcgagcag ccccagcagg ttgaggaggg tcgacttgcc ggagccggag
      541 ggccccgcca ccgccacgaa ctcgcccggc ttcacctcca cgtccacgcc gtcgaggacc
      601 gggatcggtc caccgtcggg cccgcggaag gtcttgccga gaccgatcgc ccgtacgagc
      661 accgggggct cgctcaggtc ccccgccgga tggcctcggc gggagacagc cgcacgatcc
      721 gcagggtggc gtaggtggcg gccagaccgc ccaggatcac cgagaacgcg atcgcgaaga
      781 ccaggaggcg cggggagaag aggaagagct gctggccgtc tcgcagcacg cgcgcgtcca
      841 ccaaggtccc gatggccagg gccaggccca ccccgagggc gccgccggag atggagacgc
      901 cgagggcctc gcccaagatc tcgcgcagga gctggagatc ggtggcgccg agcgcgcgct
      961 tgatgccgaa gtcgcggatg cgctcgaaga ccgccgcggt cacggtgttg gagagcgaca
     1021 ggccgccgat cacgagcccc agcaccccga tgccgaacag cagcgcggag aagaaggcgg
     1081 tggactggcg caggagccgg ctcagctcgc ccggaatcgt gacgttcact cccggcaccc
     1141 cggcctggat gcgccgggcg aggtcgtcgg cgtccacccc gtcggcccac gccaccgcgg
     1201 ccccggtgtt gaggtcgcgg cggcccatcc cggcgccgcc cgcgaagacc tggaccagca
     1261 gcgggtcgcg acgcagccag agatcgcgcg catcgtcgag cgggatgatc gcgaagcggt
     1321 ccggggcggt gagcgtcttg tcgagcaccc cgaccacctc gaaggtacgt ccggcggcca
     1381 gctcgagccg gccgcccacc gcgagctttc gggtggctgc gaagtcggcg cccaccaccg
     1441 cgaccccacg gtcgttcgcg cgcaggaaac ggccgctctt caccggcagc tcgcggaact
     1501 ggcggttggg catcggtgtc gcggggtcga tgccgaggac cagctcctgg gtcagggtca
     1561 ggaactgcga ggtggtgggg ttgagcggca gcatcacctg cgcctgtacc gcggccaccc
     1621 ccggcacctg cgcgatctgc tcgatcttgg cggcccgcag cagaccgccc gcggtgaagc
     1681 cggtgcccat ccccatgccc tcgccggcca ccgagatctg gcccagcacg aagcggtcgc
     1741 cgccgtcgat gaagcgggtg atgcgctcgg ccatcgcgcc gagcgcgacg aggccggata
     1801 cgcccaccgc gatgcccagg atcgtgaaga ggtagttctt gggcaggagg cgggcgatcc
     1861 ctaccatttg aagcgagact cggtggcgag accccggcga cggcgcaccc agcgattgac
     1921 gcggacaacg gtgaagaaga cctcgagcga cagccgcatc cggagcgaca tgcggagaaa
     1981 cgcgccgccc gccagcaccc cggtgacccc gtccaccatg cccgcgaact cgcgcggatt
     2041 gaggaacacc tcggtgaacg acgggtcgta gaaattccgg atgaaccgct ggaatggctg
     2101 catgccccgc cggatcgcct tctggtaggc gcggaagcga cgcgccgcga agcggttctc
     2161 ccggaacgcc ttgaggatct cggccgagga gagctcggcg gactgggtgg cgaggtagac
     2221 gcccgacgag aagatcgggt ccaggaacgc gatcgcgtcg cccacgcaca cgaagcggtc
     2281 gccgacggcg ggctcggtct ggtaggagaa gttggccgcg ctgtgcaccg gggtgatgcg
     2341 cggggcgccg tcgagcgcgt ccaccaggcc gcgacagcgc gcgatcagcg actcgtagag
     2401 ctgctcgaga ttgccctcgc gcccgcggac ggtgcgcgcg tggagcacgc agcccacgct
     2461 gctggtgccg ttgtggaacg ggatgtacca gaaccagccg ggctcgaagc tgaagatccg
     2521 gatgttgccc gcctccttcc cgggccagcg cttgccgccc tggaagtggg cgaacagcgc
     2581 gaccttgccg agcccctcga tggggcggcg gcgcccgtgc cgggtcgcga gaaaggcgtc
     2641 gcgcccgctc gcgtcgacca ggaagcgcgc gcggatctcc cgggagcccg cgccctcggc
     2701 gacggtggcg gtcacgccgg cgtcgtcgaa ggccacccgc tcgacggtgg cgggctggag
     2761 cagcgtcacg ttcggctgcc gggccgcgtg gtcgagcagg atcttgtcga actcgccccg
     2821 ctggacctcg taggtgtagg agggccacgg cttgccctcg cggaagaaga actggtagcc
     2881 gagatccatg tcctggtcgt ggaagtaggc cccgtacttc acgaggaagc cgcgcgcctt
     2941 caccgcgtcg agcacgccga gccgctccag caccggcatg ttggcgggca gcagggattc
     3001 ccccacgtga aagcgcggga acgcctcgcg ctcggcgagg gccacccgca gcccgccgcg
     3061 cgagaggaag gtcgccgcgc tcgagccccc cggcccgccc ccgaccacca gcacgtcgta
     3121 gtcggcgggc cgcgcggccc cggccgcgcg ccgcgcgggc gcctcgagcg tcgcggccat
     3181 cgccccctgc ggatagaggt tccgcaccac cgggccgagc cagtcgatgc cgagggcggc
     3241 cagcgcgctc cggccgagcg ccagcacgag cagcagcggg gtcgccgcgg tggcgaccgc
     3301 ggccatgcgc aggccacggg cgtgatcgga gcccaagccg cgctccacgg tgcggagggc
     3361 ccggtccgcg acgaaggcgc ccaccgcgag ggcccagccc ggcgggtgcg accggtgcgg
     3421 ggccgcgaac cggtacatgt cgcggtgccg cgcccgcagc cgggcgggac tgaagatgcc
     3481 gaccgcccac gcggcgccgg cggcggcctc gcgcgccca
//
LOCUS       DATMCV010000514         3933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_430070, whole genome shotgun sequence.
ACCESSION   DATMCV010000514 DATMCV010000000
VERSION     DATMCV010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430070"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VNU03_11840"
     CDS             complement(<1..147)
                     /locus_tag="VNU03_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease"
                     /protein_id="HWP75200.1"
                     /translation="MESIILGWYVMVHTGSVLLLTVFGSLQFLGTLAAPMFGVLGDRV
                     GARLM"
     gene            complement(222..1298)
                     /locus_tag="VNU03_11845"
     CDS             complement(222..1298)
                     /locus_tag="VNU03_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75201.1"
                     /translation="MTTRRAFLVEAAGAVAGLAFVGCDLLKARPAAAQTARRMVSVAG
                     RRVKTIDVHAHAAVPEAMALMNPPVGPPNLLPKLLMGAPQERLALMDAQGIDVQALSI
                     NPYWYKADRDVATRVCEIQNEKLAQVCAANPERFVAFATVALQHPDLAAAQLEEGVKK
                     YNLRGAAIGGSVDGLEISDPRFHPFWARAEQLGVPVFIHPQGTAELNATSRFKGNGLL
                     DNVIGNPLETTIALSHLIFEGTLDRFPGLKICAAHGGGFLPSYAGRSDQGCLTFPTRC
                     TGPLKKKPSEYLRQMYYDSIVFTPEALRHLAAETGPGQIVMGTDYPYPWTSTSVDHIL
                     SAPGFSDPERAAMLGDTAAKLLKI"
     gene            complement(1361..2380)
                     /locus_tag="VNU03_11850"
     CDS             complement(1361..2380)
                     /locus_tag="VNU03_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75202.1"
                     /translation="MARSAARFGIVLLPETLTGWGDLCREVEDVGFEWLGVADSQSVF
                     REMYVALTVAALHTRGVRLGPLVTNPLTRHLVVTASAIASVDELSGGRAVLGLGSGDS
                     AIYTIGAPPATLAGLEDSIATLGRLTAGETVERAGRPWRVHRSTRRVPIYLAAEGPRT
                     LELAGRVADGVIVGLGLTPEVVRLSLAAIERGARAAGRTLDDVDVWWFAKTSLADRRE
                     QAIEPIKMALAASANHAFRFTLEGKGVPPDLHEKIRALQREYNSHHHEIPGAANASLP
                     DRWDLTDFLVDRFAIAGTPADCVAQVERAMDAGARQFLITGFVPDPRVFMRRWAREVA
                     DRVTV"
     gene            2499..3116
                     /locus_tag="VNU03_11855"
     CDS             2499..3116
                     /locus_tag="VNU03_11855"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HWP75203.1"
                     /translation="MIYEFRTYTLKPRSLGEVEKRYAEAYEYRKKYSPLAAFWHTEVG
                     PLNEIIHVWGYKDLAERARIRAEAAKETNWPPKIRDFIVDQNVEVLNPFPFIPDVTPG
                     TLGPIFEIRRYTLAAGSLPGVMKRWEAALPARIKLSPLVTAGGMEFGGANRFIHIWAY
                     QTMDQRLQVREQGRKAGVWPPPGGGDELLAQETKIVMPSAFSPLQ"
     gene            3127..3669
                     /locus_tag="VNU03_11860"
     CDS             3127..3669
                     /locus_tag="VNU03_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006138312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP75204.1"
                     /translation="MPPTKLAHLVFQTNRLPAMRDWYCTVLDGHVIYENPHLSFVTYD
                     DEHHRVAFIDFGPLAAREVGGSDLRYRSTDQPGLHHVAFTFGSMGELLDNYERLAARG
                     IRPFRSINHGPTTSMYYVDPDGNRVELQIDNFATAEEGQAWMHSPAFDQNPIGVEYDP
                     DDLVRRFRAGVPVDELVIRD"
     gene            3678..>3933
                     /locus_tag="VNU03_11865"
     CDS             3678..>3933
                     /locus_tag="VNU03_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75205.1"
                     /translation="MTSTALPPSAVAALLHSAAAVIHAELSALPAPALGFHPAEGEWC
                     AKEVLGHLIECEKLGFSGRIHVIMQTEASPQLEGWDQDAVA"
BASE COUNT          598 a         1375 c         1407 g          553 t
ORIGIN      
        1 catgaggcgg gcgcccaccc ggtcgcccag caccccgaac atcggcgcgg cgagggtgcc
       61 gaggaactgg agcgacccga agacggtgag gaggagcacc gagccggtgt ggaccatcac
      121 gtaccagccg aggatgatcg attccatctc ggaagcccac gacgtcaaga ggtcggcagg
      181 ccactggtat cggaagctgc ggacgcggaa cgcggcgagg atcagatctt caggagcttc
      241 gccgcggtgt cgcccagcat cgcggcccgc tccggatcgc tgaagccggg cgcgctcagg
      301 atgtggtcca ccgacgtgct cgtccaggga tagggatagt cggtgcccat gacgatctgg
      361 cccgggccgg tctcggcggc caggtgacgg agggcctccg gggtgaagac gatcgaatcg
      421 tagtacatct ggcgcaggta ctcggagggc ttcttcttga gcgggccggt gcagcgcgtc
      481 gggaacgtca ggcagccctg gtcggagcgc cccgcgtagg agggcaggaa gcccccgccg
      541 tgggccgcgc agatcttgag gccggggaag cggtcgaggg tgccctcgaa gatgaggtgc
      601 gagagcgcga tggtggtctc gagcgggttg ccgatcacgt tgtcgaggag cccgttgccc
      661 ttgaagcggg aagtggcgtt cagctccgcg gtgccctgcg ggtggatgaa caccggcacc
      721 ccgagctgct cggccctggc ccagaacgga tggaaccggg gatcggagat ctccaggccg
      781 tcgacgctgc ccccgatggc ggccccgcgc aggttgtact tcttcacgcc ctcctcgagc
      841 tgggccgccg cgaggtcggg atgctgcagg gccacggtgg cgaaggccac gaagcgctcc
      901 gggttggccg cgcagacctg ggcgagcttc tcgttctgga tctcgcagac acgggtcgcg
      961 acgtcgcggt ccgccttgta ccagtagggg ttgatgctga gcgcctgcac gtcgatgccc
     1021 tgcgcgtcca tcagggcgag ccgctcctgc ggcgcgccca tgagcagctt cgggaggagg
     1081 ttgggcgggc ccaccggcgg attcatgagg gccatcgcct cggggaccgc ggcgtgcgcg
     1141 tgcacgtcga tggtcttgac gcgacggccc gccaccgaga ccatccggcg cgcggtctgc
     1201 gcggcggcgg ggcgcgcctt cagcagatcg catcccacga acgcgagtcc cgcgacggcg
     1261 ccggcggctt cgacgaggaa cgctctgcgc gtggtcatga gtctctccgt ggtgacgacg
     1321 ctgtgcaccg cacggatcgg gcgcgacgcg ggcgagccta tcacacggtg acgcggtccg
     1381 cgacctcccg ggcccaccgg cgcatgaaca cgcgcgggtc cggcacgaag ccggtgatga
     1441 ggaactggcg cgcccccgcg tccatcgcgc gctcgacctg ggccacgcag tcggccggcg
     1501 tgcccgcgat cgcgaagcgg tcgaccagga agtcggtcag gtcccagcgg tcgggcaggc
     1561 tcgcgttggc cgcccccggg atctcgtggt ggtgggaatt gtactcgcgc tgcagcgccc
     1621 ggatcttctc gtggagatcg ggcggcaccc ccttgccctc gagcgtgaag cggaaggcgt
     1681 ggttcgcgct cgcggccagc gccatcttga tgggctcgat cgcctgctcg cgccggtcgg
     1741 ccaggctggt cttggcgaac caccacacgt cgacgtcgtc gagggtgcgg ccggccgctc
     1801 gcgccccgcg ctcgatggcg gcgagcgaca ggcgcaccac ctccggggtc agcccgagcc
     1861 cgacgatcac cccgtcggcc acgcggccgg ccagctcgag ggtgcgcggc ccctccgccg
     1921 cgaggtagat cgggacgcga cgggtcgagc ggtggacgcg ccagggccgg cccgcgcgct
     1981 ccaccgtctc gccggcggtg aggcggccga gcgtcgcgat cgagtcctcg agccccgcga
     2041 gggtggcggg cggcgcgccg atggtgtaga tcgcgctgtc gcccgagccc aggcccagca
     2101 ccgcccgccc gccggagagc tcgtccacgc tcgcgatcgc gctcgcggtg accacgaggt
     2161 gccgcgtgag cgggttggtg acgagcgggc ccaggcgcac cccgcgggtg tggagcgcgg
     2221 cgacggtgag cgcgacgtac atctcgcgga agaccgactg cgagtcggcc acgcccagcc
     2281 actcgaagcc gacgtcctcc acctcgcggc agaggtcgcc ccagccggtg agcgtctccg
     2341 ggaggaggac gatgccgaac cgcgccgccg agcgcgccat gaccggccac accctagcac
     2401 cggctccgcc ttgacgtctc cggttttggg gggcagactt cccgagtgcc gaggacgatg
     2461 cagccgatgg tcacgcggtc ccccaaagga gggcggtcat gatctacgag ttccgtacct
     2521 acacgctcaa gccccgcagc ctgggcgagg tcgagaagcg ctacgcggag gcctacgagt
     2581 accggaagaa gtactcgccg ctcgccgcct tctggcacac cgaggtggga ccgctcaacg
     2641 agatcatcca cgtgtggggc tacaaggacc tcgccgagcg cgcccgcatc cgggccgagg
     2701 cggccaagga gaccaactgg ccgcccaaga tccgcgactt catcgtggac cagaacgtcg
     2761 aggtgctgaa ccccttcccg ttcatccccg acgtcacccc gggcacgctg ggaccgatct
     2821 tcgagatccg ccgttacacc ctggccgcgg gctcgctgcc cggggtcatg aagcgctggg
     2881 aagccgcgct gccggcgcgc atcaagcttt cccccctggt caccgccggc gggatggagt
     2941 tcggcggcgc gaaccgcttc atccacatct gggcctacca gaccatggac cagcggctgc
     3001 aggtgcggga gcaggggcgc aaggcaggcg tctggccgcc gccgggcggc ggggacgagc
     3061 tgctcgccca ggagaccaag atcgtgatgc cgtcggcgtt ctcgccgctc cagtagggag
     3121 gcgcccatgc caccgaccaa gctcgcccat ctcgtcttcc agaccaatcg cctgcccgcg
     3181 atgcgggact ggtactgcac ggtgctcgac gggcacgtga tctacgagaa cccgcatctg
     3241 tcctttgtga cctacgacga cgagcaccac cgggtggcgt tcatcgattt cggcccgctc
     3301 gcggcccgcg aggtcggagg gagcgatctc cgctatcgct ccaccgacca gcccgggctc
     3361 caccacgtcg cgttcacctt cggctcgatg ggcgagctgc tcgacaacta cgagcggctc
     3421 gccgcgcggg gcatccggcc gttccgctcc atcaaccacg ggccgaccac gtccatgtac
     3481 tacgtcgatc ccgacggcaa ccgcgtggag ctgcagatcg acaacttcgc caccgcggag
     3541 gaggggcagg cctggatgca ctcccccgcc ttcgaccaga acccgatcgg cgtcgagtac
     3601 gaccccgacg acctggtccg ccgcttccgg gccggggtcc cggtcgacga gctggtgatc
     3661 cgcgactagg ggtcgcgatg acatccaccg ccctgccacc gtcggccgtc gccgcgctgc
     3721 tgcactccgc ggccgcggtg atccacgcgg agctctcggc gttgcccgcc cccgcgctgg
     3781 gattccaccc ggccgagggc gagtggtgcg cgaaggaggt gctcggtcac ctgatcgagt
     3841 gcgagaagct cggcttctcg gggcggatcc acgtcatcat gcagaccgag gccagcccgc
     3901 agctcgaggg ttgggaccag gacgcggtcg cgc
//
LOCUS       DATMCV010000515         2758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_25283, whole genome shotgun sequence.
ACCESSION   DATMCV010000515 DATMCV010000000
VERSION     DATMCV010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25283"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1206
                     /gene="rlmN"
                     /locus_tag="VNU03_11870"
     CDS             172..1206
                     /gene="rlmN"
                     /locus_tag="VNU03_11870"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWP75206.1"
                     /translation="MSSTNLLDCSPAELEALAESLGAPRYRGRQLARWIFARGVVDLE
                     AMTDLPRDFRTALAARATVDVPEVERRTPSQDGSHKLVLRYGDGARVQAVLMPDGDRL
                     TLCVSTQVGCGFACAFCFTGTMGLERNLSAGEIVAQVLVARAGLGPGERITHIVYMGM
                     GEPLANYSATVKSLRLLTDPQAFAFSPRRITVSTVGLVSGIERLAKETLKVNLAISLH
                     ATSNEIRDRIMPVNRGFAIEELLAACRRFPLPFRQRMTFEYVLLDGINDSVEDARRLV
                     KLLKGIRGKINLIPFNDWEGSSFSRPPLPRILAFQAVLLEHGITATIRWSKGEDIGAA
                     CGQLREEVPA"
     gene            1203..2528
                     /gene="mtaB"
                     /locus_tag="VNU03_11875"
     CDS             1203..2528
                     /gene="mtaB"
                     /locus_tag="VNU03_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HWP75207.1"
                     /translation="MSAPVTVALTTLGCRLNQVESQEMGALLEGRGFRVVPAGSRAQV
                     HVVNTCTVTGRADFSDRQIIRRIGREDPGAFLVVTGCYAQTDPDTVAALPGVDLVVGN
                     QEKYRLPDLLDSLVKRARPTVAVADIREARSVPVAPVTRMTGRSRPFVKIQDGCQHRC
                     AFCIVPAARGGSRSQEPKVVLDQVRALAEAGYPDVTLTGVDIGHYGWDLYPRTSLAAL
                     VRSLAGVAGLRWLRLSSVLPSYFTDDLVDVVATLPVVAPHLHLPLQSGSDRVLRLMRR
                     PYHTGMYRALVDRLAAAIPGLGLGADVIVGHPGETDEDFEATLALVGSLPFSYLHVFT
                     YSDRKGTEAARRPGHVPAAVIRERSQRLRRLGREKSHAFRSALVGSAREVLVLEARDH
                     QTGLLSGLTSNYVEVLFPGPDGLGRRMATVEIAEVRGERTRGRLVEVPA"
     gene            2597..>2758
                     /locus_tag="VNU03_11880"
     CDS             2597..>2758
                     /locus_tag="VNU03_11880"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941773.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     5'-deoxy-5'- methylthioadenosine (MTA) to adenine and
                     5-methylthio-D-ribose-1- phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWP75208.1"
                     /translation="MEGIEDLRWVRVRTPFGEASDQFGTGRLGDRRVIFLPRHGRGHR
                     LSPTEINYRA"
BASE COUNT          348 a         1006 c          988 g          416 t
ORIGIN      
        1 catgcgcttc gtgtggacgt acctcgtggt ggtcgtggtg ttcgcgttcg gatttctctg
       61 cgcttccgcc ttcacgctgg gcgacgacta gccgctgcgt ccggccgccc tcgcggagca
      121 ggcgggccgt gcccggtgag ccctccatcc ctgtgctact atccgacgcg tatgtcttcg
      181 acgaacctgc tggattgctc gcccgccgag ctggaagcgc tggccgagtc gctgggagcc
      241 ccccggtacc gcgggcggca gctggctcgg tggatcttcg cccggggagt ggtggatctc
      301 gaggcgatga ccgacctgcc gcgcgacttc cgcaccgcgc tggcggcccg ggcgacggtg
      361 gacgtgccgg aggtcgagcg ccggaccccg tcgcaggacg gtagccacaa gctcgtgctc
      421 cgctacggcg acggcgcccg cgtccaggcg gtgctgatgc cggacggcga ccgcctcacc
      481 ctctgcgtct ccacccaggt gggctgcggc ttcgcctgcg cgttctgctt caccggcacc
      541 atggggctcg agcgcaacct gagcgcgggc gagatcgtgg cccaggtgct ggtggcccgc
      601 gcggggctcg gcccgggcga gcggatcacc cacatcgtgt acatggggat gggcgagccg
      661 ctcgctaact actcggccac cgtgaagtcg ctccgcctgc tgaccgaccc gcaggccttc
      721 gccttctcgc cccgacgcat cacggtgtcg acggtcgggc tcgtgagcgg tatcgagcgg
      781 ctggccaagg agaccctcaa ggtcaacctg gccatctcgc tgcacgccac cagcaacgag
      841 atccgcgacc ggatcatgcc ggtcaatcgc ggcttcgcca tcgaggagct gctcgcggcc
      901 tgccggcgct ttccgctgcc gttccgccag cgcatgacct tcgagtacgt gctgctcgac
      961 ggcatcaacg actcggtcga ggacgcccgc cggctcgtga agctgctgaa ggggatccgc
     1021 ggcaagatca acctgatccc gttcaacgac tgggaaggct cgagcttctc gcggcccccg
     1081 ctgccccgca tcctcgcgtt ccaggcggtc ctgctcgagc acgggatcac cgcgaccatc
     1141 cgctggagca agggggagga catcggggcg gcgtgcggcc agctccgcga ggaggtgccg
     1201 gcatgagcgc gccggtgacg gtggccctca ccacgctcgg ctgccggctc aaccaggtgg
     1261 agtcgcagga gatgggcgcg ctcctcgagg ggcgcggctt ccgggtggtg cccgcgggca
     1321 gccgcgcgca ggtccacgtg gtgaacacgt gcacggtgac cgggcgagcc gacttctccg
     1381 accgccagat catccgccgg atcggccgtg aggacccggg cgccttcctc gtcgtgaccg
     1441 gctgctatgc ccagaccgat ccggacacgg tcgccgcctt gcccggcgtg gacctggtgg
     1501 tcggcaatca ggagaagtac cggctgcccg acctgctcga ctcgctggtc aagcgcgcgc
     1561 ggcccacggt ggcggtggcc gacatccgcg aggcccgctc ggtcccggtc gcgccggtca
     1621 ctcggatgac cggccggtcg cgccccttcg tgaagatcca ggacggctgc cagcaccgct
     1681 gcgccttctg catcgtgccc gccgcgcgcg gcgggagccg gagccaggag ccgaaggtcg
     1741 tgctcgacca ggtgcgcgcg ctggcggagg ccgggtatcc ggacgtcacg ctgaccggcg
     1801 tcgacatcgg ccactacggc tgggatctct acccgcggac gtcgctcgcg gccctcgtgc
     1861 ggagcctcgc cggcgtcgca ggcctgcgct ggctccgcct ctcctcggtg ctgccgtcct
     1921 acttcaccga cgatctcgtc gacgtggtcg ccacgctgcc ggtggtggcg ccgcacctgc
     1981 atctcccgct ccagagcggg agcgaccgcg tgctgcgcct catgcggcgg ccgtaccaca
     2041 ccgggatgta ccgcgcgctg gtggaccggc tggccgccgc catccccggg ctcgggttgg
     2101 gcgccgacgt catcgtcggg caccccggcg agaccgacga ggacttcgag gccacgctcg
     2161 cgctcgtcgg ctcgctgccc ttctcttacc tgcacgtctt cacctactcg gaccgcaagg
     2221 gaaccgaggc cgcgcggcgc ccgggccacg tgcccgccgc ggtcatccgc gagcgcagcc
     2281 agcggctgcg gcgtctcggc cgggagaaga gccacgcctt ccggagcgcg ctcgtcggct
     2341 cggcgcgcga ggtcctggtg ctggaggcgc gggaccacca gaccgggctg ctgtccggcc
     2401 tcacgtccaa ctacgtcgag gtgctgttcc cggggccgga cggtctcggg cggcgcatgg
     2461 cgacggtgga gatcgccgag gtccgcggcg agcggacgcg cgggcgcctc gtggaagtgc
     2521 ccgcgtgagc gcccggcgcg cggcggcccc gcccgagccg gcgctcggca tcatcggcgg
     2581 cagcgggctc tacgagatgg agggcatcga ggacctccgc tgggtccggg tgcgcacgcc
     2641 cttcggagag gcgtccgatc agttcggcac cggccggctc ggggatcggc gggtcatctt
     2701 cctgccccgg cacggtcgcg gccaccggct gagcccgacc gagatcaact accgggcg
//
LOCUS       DATMCV010000516         5390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_25298, whole genome shotgun sequence.
ACCESSION   DATMCV010000516 DATMCV010000000
VERSION     DATMCV010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25298"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..671
                     /locus_tag="VNU03_11885"
     CDS             165..671
                     /locus_tag="VNU03_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWP75209.1"
                     /translation="MASVEYRGMNLVIPENDSEWREHFKLARTNHKLMLRACTQCKLM
                     RYPPSHGCPWCMSLEWEWQAVSGRGHIYSYEVVHHAIQPGFKEWTPYAVVLVELEEQR
                     GQPTPDEALRVIANLVTPEFRPEPEQNVAIGKRVRVVFQDLSADFALPQFTLTDEPAE
                     GRAWRFPG"
     gene            complement(619..1428)
                     /locus_tag="VNU03_11890"
     CDS             complement(619..1428)
                     /locus_tag="VNU03_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75210.1"
                     /translation="MTLLLKLVLVPGLIALVTMAGRRFGPRIGGWLNALPLVAGPVLF
                     FLALEQGDRFVAQAALSTLAGLAAVAAFAVIYSWTAVARPWWVCVLVGWIAFAGLTVA
                     LQAVRWTAGAGLALALAAFGIAPLVLPALPDAPPPEAAPAWDLPLRMTAAVALVLLVT
                     GLAGWLGPRWSGAITPFPIATTILLAFTHAQQGAPAAVGFLRAFLPAMWSFALFCFVL
                     ACGVVGLGRDLAFLLALGVSLAVQGVVWLGLASRASRGSARPGPRPARRSA"
     gene            complement(1443..1976)
                     /locus_tag="VNU03_11895"
     CDS             complement(1443..1976)
                     /locus_tag="VNU03_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1692..1694),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HWP75211.1"
                     /translation="MSDEEFLDPTDSVAVPRKTAPRLETLEGKTITLLDISKAKGNHL
                     LDRVEELLRERAHPRDVVRRKKPTFARPAPDDLRQEILKNSDAVIEALADUGSCTTCS
                     VHDSVFFEDQGIPTATVISTEFKRAARAQADALGARDHQTVMVAHPIQPLTRDEVRAL
                     ADKAYDEIVARLTRPGR"
     gene            complement(1973..3025)
                     /locus_tag="VNU03_11900"
     CDS             complement(1973..3025)
                     /locus_tag="VNU03_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75212.1"
                     /translation="MADPGDPFEALYARGVTDGLPVVPPTRERVAAAVAASGRPGDAL
                     VALVPPNFGRATVEKIATNAVMAGCRPEYLPVVVAGVEAMCDEGFDLHGVSATTNFPS
                     PLFIVNGPARERLDINCGAGLFGPGWRANATIGRALRLIAVNLGGAIPGVVSMSTMAH
                     PGRYTYCIGEAEEENPWDSLAVEHGFAPGESVIAAFAGEAPHGVYDHMSRTARDLLIT
                     ISKSLEVISHHKSTHHGDTLVVLSPEHARTIAGDGWGKAEMRDFLWTRLRKPVRELVP
                     GVEGGEGLPAHVLAKFPHPATDETLIAKFREPGNLKFVVAGGTAGRFSAVIPGWTFPK
                     SSRMIWRKIDMPEEGS"
     gene            complement(3038..3703)
                     /locus_tag="VNU03_11905"
     CDS             complement(3038..3703)
                     /locus_tag="VNU03_11905"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HWP75213.1"
                     /translation="MVAPRALILLLVVALSGAGVGSAPGAECVALDDFSRGAIGEFPP
                     DWKPRKDAGRPVYSIQEEAGQRFLHAAATRLGIQAGREVAWNLDVYPILAWSWRPIEF
                     PAGSDERKSSTNDSAVSVYAVFPGSAVSVKSVKYVWSRVVPVGTHFTSSAGDTQARVL
                     RTGTDRVGQWVEEQVNVREDYRKYFGGSDVPRPAGIAVLTDSDDTKSTARGDYASFRV
                     CRP"
     gene            complement(3682..5091)
                     /locus_tag="VNU03_11910"
     CDS             complement(3682..5091)
                     /locus_tag="VNU03_11910"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWP75214.1"
                     /translation="MDQRILEFIGDLRRAEMRISPSEALDALTASTEIGLEDRETFKS
                     ALATTLVKESRDLVTFNRIFDLYFLDLQALGEGLKKALGPEDPKIQEMLDRLTSEAGM
                     EMDELTELLLAGEGSDMEMAIRQEGQGTGLERLMYFLQVGYFSRRIYDKFDWEAIERD
                     LGRIMQLLESKGLDPGMIARIRNYLDLRLEAFRRMIRQHVERELERRAYRQGEKLTRE
                     VLTDKPLFALSADEVAQMKAVVARLARKIKDALALRQRQEEKGRLDSRRTIRQSLQYG
                     GVPMEIRFRRRHREKPKLVTLCDVSDSVRNASRFMLQLVWSLQECFSRVRSYVFVSEI
                     AEVTQAFATYPVDTAIDWALKSSSVDYHCRSDFGYAFSRFVRTDLDSLDRKTTVLILG
                     DARNNYNDPQAWAIRQIRERVKGIIWLNPEGQWGWGIGDSVMPLYTPACDIVKECRTV
                     GQLVQVVDQLVHSWWRRGR"
BASE COUNT          826 a         1881 c         1910 g          773 t
ORIGIN      
        1 cttcgcggtc aggtcgacga ctactgcccg ggctgggagg acggcaagca cagctacgac
       61 tactcgccgg gcaagtgccg ccaggtgaag gatgcgcgcg tcaccatgaa cctcggctgg
      121 gcgatgccgc cgaccggctc cgccctcatc atgaggaact agcgatggcc tccgtcgaat
      181 accgcggcat gaacctggtg atcccggaga acgactccga gtggcgcgag cacttcaagc
      241 tcgcccgcac caaccacaag ctgatgctcc gcgcctgcac gcagtgcaag ttgatgcgct
      301 acccgccgag ccacggctgt ccgtggtgca tgtcgctcga gtgggagtgg caggcggtga
      361 gcggccgggg ccacatctac tcctacgagg tggtgcacca cgccatccag cccgggttca
      421 aggagtggac cccgtacgcg gtggtgctgg tggagctcga ggagcagcgc gggcagccga
      481 cgcccgacga ggcgctgcgg gtcatcgcca acctggtcac tcccgaattc cgtccggagc
      541 cggagcagaa cgtggccatc ggcaagcggg tgcgggtggt gttccaggac ctctcggccg
      601 acttcgcgct gcctcagttc acgctgaccg acgagccggc cgagggccgg gcctggcgct
      661 tccccggcta gcccgcgacg cgaggccgag ccacaccacg ccctgcaccg ccaggctcac
      721 gccgagcgcg agcaggaacg cgagatcgcg ccccagcccc accaccccgc aggcgagcac
      781 gaagcagaac agcgcgaagc tccacatcgc gggcaggaac gcgcgcagga atcccaccgc
      841 ggccggggcg ccctgctggg cgtgggtgaa ggcgagcagg atggtggtcg cgatcgggaa
      901 cggggtgatc gcgccgctcc agcggggccc gagccagccg gccaggccgg tgaccagcag
      961 gaccagggcc accgcggcgg tcatgcggag cggcaagtcc caggcgggcg cggcctcggg
     1021 cggcggcgca tccgggagcg cgggcagcac cagcggcgcg atgccgaacg cggcgagggc
     1081 gagcgcgagg cccgcgccgg cagtccagcg caccgcctgc agcgccaccg tgaggcccgc
     1141 gaaggcgatc cacccgacga gcacgcacac ccaccagggc cgcgccaccg ccgtccacga
     1201 gtagatcacc gcgaaggccg ccaccgcggc gagaccggcg agggtggaga gcgcggcctg
     1261 cgccacgaat cgatcgccct gctcgagggc caggaagaag agcaccggcc ccgcgaccag
     1321 cggcagggca ttgagccagc ccccgatgcg cgggccgaag cgacggccgg ccatggtgac
     1381 cagggcgatg agccccggca ccaggaccag cttgaggagg agggtcaatc tatcgggtcg
     1441 acttatcgac cggggcgggt cagccgggcg acgatctcgt cgtaggcctt gtccgccagc
     1501 gcgcgcacct cgtcccgggt gaggggttgg atcgggtggg cgaccatcac ggtctggtga
     1561 tcacgggcgc cgagtgcgtc ggcctgggcc cgcgccgccc gcttgaactc ggtcgagatg
     1621 acggtggcgg tgggaatccc ctggtcctcg aagaacacgc tgtcgtgcac actgcacgtt
     1681 gtgcaggacc ctcagtcggc gagggcctcg atcaccgcgt cgctgttctt caagatctcc
     1741 tggcgcaggt cgtcgggcgc cgggcgcgcg aacgtcggct tcttccggcg cacgacgtcg
     1801 cggggatggg cccgctcgcg cagcagctcc tcgacgcggt cgagcagatg gttccccttg
     1861 gccttggaga tgtcgaggag ggtgatggtc ttgccctcga gggtctcgag gcggggggcg
     1921 gtcttgcggg ggaccgccac cgagtcggtc ggatccagga actcttcgtc gctcatgatc
     1981 cctcctccgg catgtcgatc ttcctccaga tcatgcgcga gctcttggga aacgtccagc
     2041 ccggaatgac cgccgagaag cgccccgcgg tgccccccgc caccacgaac ttgagattgc
     2101 ccggctcgcg gaacttggcg atcagcgtct cgtcggtcgc gggatgcggg aacttggcga
     2161 gcacgtgggc aggcagcccc tcgccgccct cgacgcccgg gaccagctcg cgcaccggct
     2221 tgcgcaggcg cgtccacagg aagtcgcgca tctccgcctt gccccagccg tcgcccgcga
     2281 tggtgcgggc gtgctcgggc gaaagcacca cgagggtgtc gccgtggtgg gtcgacttgt
     2341 ggtgcgagat cacctcgagc gacttcgaga tcgtgatcag cagatcgcgc gcggtgcggc
     2401 tcatgtggtc gtacaccccg tgcggcgcct cccccgcgaa ggcggcgatc acgctctcgc
     2461 cgggggcgaa gccgtgctcg accgcgaggg agtcccacgg gttctcctcc tccgcctccc
     2521 cgatgcaata ggtgtagcgc ccggggtggg ccatcgtgga catgctcacc accccgggga
     2581 tggccccgcc caggttcacc gcgatcagcc ggagcgcccg cccgatggtg gcgttggcgc
     2641 gccagccggg cccgaacagc cccgccccgc agttgatgtc gaggcgctcg cgcgcgggcc
     2701 cgttgacgat gaagagtggc gaggggaaat tggtggtggc cgacaccccg tggaggtcga
     2761 agccctcgtc gcacatcgcc tccacccccg ccaccaccac cggcaggtac tcgggccggc
     2821 agcccgccat caccgcgttg gtcgcgatct tctcgacggt ggcgcggccg aaattgggcg
     2881 gcaccagcgc gaccagcgcg tctcccgggc gcccgctcgc cgccaccgcg gcggccaccc
     2941 gttcgcgggt ggggggcacc accggcaggc cgtcggtgac cccgcgcgcg tagagcgcct
     3001 cgaacggatc gccgggatca gccacccgga cggggcctca tggcctgcac actctgaagg
     3061 acgcgtagtc gccgcgcgcg gtgctcttgg tgtcgtcgga gtcggtgagc accgcgatgc
     3121 ccgccgggcg cggaacgtcc gacccgccga agtacttccg gtagtcctcg cggacgttca
     3181 cctgctcctc gacccactgc ccgacccggt cggtgccggt gcgcagcacc cgcgcctgcg
     3241 tgtcacccgc gctcgacgtg aagtgcgtcc ccaccggcac cacccggctc cacacgtact
     3301 tcacgctctt gaccgagacc gccgagccgg ggaaaaccgc gtagaccgag accgcgctgt
     3361 cgttggtcga cgacttgcgc tcgtcggagc cggccgggaa ctcgatcggg cgccacgacc
     3421 aggccaggat cgggtagacg tcgaggttcc aggccacctc gcgcccggcc tggatgccga
     3481 gccgggtggc cgccgcgtgg agaaagcgct ggcccgcctc ctcctggatc gagtacaccg
     3541 gccggcccgc gtccttccgg ggcttccagt cgggagggaa ctcgccgatg gcgccccggg
     3601 agaagtcgtc gagggccacg cactccgcgc ccggcgcgga gcccacaccg gcgcccgaca
     3661 gggcgaccac cagcaggagg atcagcgccc gcggcgccac catgaatgga cgagctggtc
     3721 caccacctgg acgagctgcc ccaccgtgcg gcactccttc acgatgtcac aggccggggt
     3781 gtagagcggc atcacgctgt cgccgatgcc ccagccccac tggccttccg ggttgagcca
     3841 gatgatgccc ttcacccgct cgcggatctg ccggatcgcc cacgcctggg gatcgttgta
     3901 gttgttgcgg gcgtcgccca ggatcagcac ggtggtcttg cggtcgagcg agtcgaggtc
     3961 ggtgcgcacg aaccggctga acgcgtagcc gaagtcggag cggcagtggt agtcgaccga
     4021 cgaggacttg agcgcccagt cgatcgcggt gtccaccggg taggtggcga aggcctgggt
     4081 gacctccgcg atctcggaga cgaagacgta agagcgcacg cgcgagaagc actcctgcag
     4141 gctccacacc agctgcagca tgaagcggga ggcgttgcgc accgagtccg acacgtcgca
     4201 gagggtgacc agcttcggct tctcgcggtg ccggcggcgg aaccggatct ccatgggcac
     4261 cccgccgtac tgcaggctct ggcggatggt ccggcgcgag tcgaggcggc ccttctcctc
     4321 ctggcgctgg cggagggcga gcgcgtcctt gatcttgcgg gcgagccgcg ccaccaccgc
     4381 cttcatctgg gccacctcgt ccgcggagag ggcgaagagc ggcttgtcgg tgaggacctc
     4441 gcgggtgagc ttctcgccct gccggtaggc gcggcgctcc agctcgcgct ccacgtgctg
     4501 gcggatcatg cgccggaacg cctcgaggcg caggtcgagg tagttgcgga tccgcgcgat
     4561 catacccggg tcgagcccct tcgactccag gagctgcatg atgcggccga ggtcgcgctc
     4621 gatcgcctcc cagtcgaact tgtcgtagat gcggcgggag aagtagccga cctggaggaa
     4681 gtacatcagc cgctcgaggc cggtgccctg gccctcctgg cggatggcca tctccatgtc
     4741 gctcccctcc ccggccagga ggagctcggt cagctcgtcc atctccatgc ccgcctcgga
     4801 ggtgagccgg tcgagcatct cctggatctt cgggtcctcc ggaccgagcg ccttcttgag
     4861 cccctcgccg agggcctgca ggtccaggaa gtagaggtcg aagatgcggt tgaaggtgac
     4921 gaggtcgcgg gactccttga ccagggtggt cgcgagcgcg ctcttgaagg tctcgcggtc
     4981 ctcgaggccg atctcggtcg aggcggtgag cgcgtcgagg gcctcgctcg gcgagatgcg
     5041 catctccgcg cgccggagat cgccgatgaa ctcgagaatg cgctggtcca tttatctcgc
     5101 ctgaacgtgg acgacttcgg tttggcggtt gggcctcttc gcccctctgg ggctcatcgg
     5161 ggcgcccgca tcaccggaag tcactcgctc atccggtttc tggcggtcga ccgtccctgc
     5221 gcggcacggt gcggcggtcg gctcatcggg cgaagtggac ccaggaggac ttgaagacgt
     5281 cctcgccgcc gtcgcgggtg atcggataca ggcggccgtc ctccagccgg atgaggccgt
     5341 ggaacgagcc ctgcgcgatc aggtgggtca tcgagagctt ccagccgagc
//
LOCUS       DATMCV010000517         6912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_163056, whole genome shotgun sequence.
ACCESSION   DATMCV010000517 DATMCV010000000
VERSION     DATMCV010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163056"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VNU03_11915"
     CDS             <1..1002
                     /locus_tag="VNU03_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP75215.1"
                     /translation="NPVFARRWRKPAEEIVGMCMADLTGKEPFEETYKPNLDRCFAGE
                     EVSFGGWFPTPGGQKYLAATYSPLRPDSERVEAVLAVTRDLTERVRASEALQEAQAEL
                     AHVTRVMTLGELTASIAHEVNQPLAAIVADANASLNWLAASPPDLGNVREALDAIVKD
                     GHRAADVIQRTRQLARKTSPQKARLDVNDVIRDVVPLIGTEMRSHEVSLRIDLAPALP
                     PVLADRVQLQQVLINFVMNGIEAMASVDGRARELVIRSQPEDDDHVVVAVQDAGVGID
                     GEKTDQLFSAFYTTKPNGMGMGLSISRSIIEAHGGRLWATPNPDHGATFHFALPRMQ"
     gene            1124..1594
                     /locus_tag="VNU03_11920"
     CDS             1124..1594
                     /locus_tag="VNU03_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75216.1"
                     /translation="MKKILGLALVAMLSLTLAAPVSAGGGRGGGFHGGGSFHGGGGFH
                     GGFHRFGCCFGPAFVGGVFVGSALGYPYYGYPYYGYPYYSYPYATYPAYADPVYAPAP
                     AYQPQTQVSVAPQIQREVCYTGGCYHLQGDGVTVAYSWVWVPSAPPAPPAPPSR"
     gene            1606..1842
                     /locus_tag="VNU03_11925"
     CDS             1606..1842
                     /locus_tag="VNU03_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75217.1"
                     /translation="MAQIAQIERPGVLARAFFKIGERMFGQVPTPERLMAQRLPLMLG
                     IGGLYGAIEWFGRIDAQLRALLNVQVAALYGVPY"
     gene            1843..2136
                     /locus_tag="VNU03_11930"
     CDS             1843..2136
                     /locus_tag="VNU03_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75218.1"
                     /translation="MDIRHAVGVKAGVPGEKLAEVLTFHESPRFSERERAALRFSERV
                     TRDDLEMSEECLAQVREHFSEAEIVELTFIIGYQTFASKFAKAWRLAPQGFSS"
     gene            2153..2569
                     /locus_tag="VNU03_11935"
     CDS             2153..2569
                     /locus_tag="VNU03_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWP75219.1"
                     /translation="MTGPIPERWAPYLQGVLRIVVAFLFVAHGTQKFMGFPSAEASAG
                     VPLMSLRGVASVIEMVGGMLLLFGLFTRPIAFILAGEMAVAYFMVHAPRGFWPLVNRG
                     EPAVFFCFTWLYFAAAGGGPWSLDALRGRSSGRASP"
     gene            complement(2606..2737)
                     /locus_tag="VNU03_11940"
     CDS             complement(2606..2737)
                     /locus_tag="VNU03_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75220.1"
                     /translation="MTDDQQDRHQNRADQGRDAALFEVRKGHDVAHESMNNPADADR"
     gene            2919..4127
                     /locus_tag="VNU03_11945"
     CDS             2919..4127
                     /locus_tag="VNU03_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75221.1"
                     /translation="MLIGAIVIASSQFIGAPRAADPIKIGWVGPLSPPGGYAEGTLMK
                     QAAQLAADEINAKGGVLGRQIQVVFADTRGKPEEGTAAAERLISQEKVVAITGEFHSS
                     VFLAEMEVAHKAGIPIIAVDVWALSITAKGYPEVFRVAPNQALIATKYGDWIAAAGFK
                     NVAFLFEKTDGGQSARDVLLPVLDKHGVKYEAVGADPNSTEFTAQIERFKTKQPPFDF
                     FMTAYSEAGAYPMISQSHTLGFAPSAKTGIGNSGGPAVDPTFWKNVGEAGKYLVTEIV
                     GLPKAAWNDKTKAFVAAYKKKYNHTPSPQAIENYDAMLVLTDAIARAKSTDGKALIAA
                     LEKTDIVLGRGRYTFSTSKQPDWAYHQFMGAPVALVQYNKVGQDAEDAPIVWPRDIAD
                     VKYMYLKPGQ"
     gene            4258..5163
                     /locus_tag="VNU03_11950"
     CDS             4258..5163
                     /locus_tag="VNU03_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75222.1"
                     /translation="MSWDYLFVQAANGAVIGVIYALIAVGVTLIFSILKIVNFAHGDL
                     YMLGGYCAYYVIEYLGFPPLIAMVVAMAALFLGAIVLERLMLTPLYSDATERKDEYGL
                     IVTFGLAYFLRNSAVIAFGPFPLKPPSFIPGVQRLGNLVVTNDRLVAAGVGIVLIAAM
                     LYFMKRTIWGQALDAVSQSRDSAAIVGINPRRFNSFAFGIGAALAAAAGALVAPIFSL
                     APDMGVLPNIHAYVIVILGGMGSVSGSIIAGILLGESENLFTAFFPDPTRAFAYTNAF
                     GVLVLMVVLVVRPQGLFGRRHLRME"
     gene            5170..6147
                     /locus_tag="VNU03_11955"
     CDS             5170..6147
                     /locus_tag="VNU03_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75223.1"
                     /translation="MRGLPVYILLAVAALALAGADRLNAYWLHVAIIAMYYGILAASW
                     SLLAGYVGLFSLSHTAFASIGGYTSALLVKWLGIPVLVGMGAGLVMCGVLGGAIGWVC
                     LRMSGPYLAIFTLAFSEIVRITISTEDDVTAGMAGLHTAPLFQSATRLPYFYAAFALL
                     AVSLLGMGLLVRSRWGLFLRAIREDEQAAGASGVAVARFRILAFAIASGFAGLAGGFY
                     AHYIGIMTPGVGDLDEMAKLVVMTVIGGMERLPTAVGGAFVLEVLLEVLRGFGQWRLP
                     LFGVLLLLTMRFARNGLLTPLWLPFVRLGETRVPPLPAARDVPQKDSAA"
     gene            6144..6869
                     /locus_tag="VNU03_11960"
     CDS             6144..6869
                     /locus_tag="VNU03_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75224.1"
                     /translation="MSQGAELVRGVGLVKRFGGIVANDAVDLVVAQGDLVGLIGPNGS
                     GKTTLINVLTGHLDPDGGEVLVRGRRVSGRPSHVYAGMGVARTFQLTQLFGRMTVLEN
                     MLVPGLTRPHSRWDEVARRARGYLEFLSLSDVAQLPARNLSGGQQKLLELGRCLMLEP
                     AVLFLDEPFAGVHPRLRDELVARIRELHQGGRTFVVVDHDMESILRISRRLVVMARGS
                     KIADGEPAVVRADQNVLAAYAGL"
BASE COUNT         1021 a         2289 c         2442 g         1160 t
ORIGIN      
        1 aacccggtct tcgcccggcg ctggagaaag ccggccgagg agatcgtcgg gatgtgcatg
       61 gccgacctca ccggcaagga gccgttcgag gagacgtaca agccgaacct ggatcgctgc
      121 ttcgcaggag aagaggtcag cttcggaggc tggttcccca cccccggcgg ccaaaaatac
      181 ctggccgcga cctattcgcc gctgcggccg gattcggagc gggtcgaagc cgtgctcgcg
      241 gtcacccgcg acctcaccga gcgcgttcgg gcgtccgaag cgctgcagga ggcgcaggcg
      301 gagctcgcgc acgtcacccg ggtgatgacc ttgggcgagc tcaccgcgtc gatcgcccac
      361 gaggtcaacc agccgctcgc cgcgatcgtg gccgatgcca acgcgtcgct caactggttg
      421 gcggcgagcc ctccggatct cggaaatgtg cgggaggcgc tcgacgccat cgtgaaggat
      481 ggtcaccggg cggcggacgt gatccagcga actcgccagc tcgcaaggaa gaccagtccc
      541 cagaaggcgc ggctggacgt gaacgacgtc attcgcgacg tcgttccgct catcggcacc
      601 gagatgcgca gccacgaggt gtcgttgcgg atagacctgg cgccggcgtt gccgccggtc
      661 ctggcggatc gggtccagct acagcaagtg ctcatcaact tcgtgatgaa tggcatcgag
      721 gccatggcat ccgtcgatgg ccgggcccgc gagctggtga ttcgctcgca gccggaggac
      781 gatgatcacg tggtcgtggc ggtgcaggac gccggcgtcg gcatcgacgg ggagaagacg
      841 gaccagctct tcagcgcctt ctacacgaca aagcccaacg gcatgggcat gggactgtcg
      901 atcagccggt cgatcatcga ggcgcatggc ggacggctct gggcgacacc gaacccggac
      961 cacggcgcga cgttccactt cgcgctgccg aggatgcaat gacgttggag caacgatcag
     1021 gaaggagaca agccctctag agccgcgata gccacgggga ggggagggaa ccacgccatc
     1081 gaccggaggg ttacaatgca gtcgtgacaa gaaggaggcg gtcatgaaga agattctcgg
     1141 tctggcgctt gtggccatgc tgtcgctcac cctcgccgcc ccggtctctg cgggcggggg
     1201 gcggggcggt ggtttccacg gtggcggcag tttccacggt ggcggtggct tccacggtgg
     1261 gttccatcgg ttcggctgct gtttcggccc ggccttcgtc ggcggggtgt tcgtcggctc
     1321 ggccctcggc tatccctact acgggtatcc ctactatggg tatccctact attcgtatcc
     1381 gtatgcgacg tatccggcct acgcggaccc cgtctacgcg ccggcgccgg cctaccagcc
     1441 gcagacgcag gtctccgtgg ccccccagat ccagcgggag gtctgctaca ccggcggctg
     1501 ctatcaccta caaggtgacg gggtgacggt cgcctatagc tgggtctggg tgccgtccgc
     1561 gccgccggcg cctccggctc cgccgagccg ctaggggagg cttatatggc acagatcgcg
     1621 caaatcgaac ggcccggcgt gctggcccgg gcattcttca agatcgggga acggatgttc
     1681 gggcaggtgc cgacgcccga gcgactgatg gcgcagcggc tcccgctcat gctgggaatc
     1741 ggggggcttt acggcgcgat cgagtggttc gggaggatcg acgctcagct tcgagccctg
     1801 ctcaatgtcc aggtggccgc cctgtacggc gtgccgtact gaatggacat ccgccacgcc
     1861 gtcggcgtga aggcgggtgt cccgggcgag aagctggccg aggtcctgac cttccacgag
     1921 agcccgcgct tcagcgagcg ggagcgggcc gcgctccggt tcagcgaacg ggtgacccgc
     1981 gacgacctcg agatgtccga ggagtgcctg gcgcaggtgc gcgagcattt tagcgaggcg
     2041 gagatcgtgg agctgacctt catcatcggg tatcagacgt ttgccagcaa gtttgcgaaa
     2101 gcctggaggc tggcgcccca gggcttctcg tcctagccgc cgaggacgaa cgttgactgg
     2161 cccgattccc gagcggtggg ccccgtatct gcagggcgtc ctccgcatcg tcgtcgcctt
     2221 tctgttcgtc gcgcacggaa cccagaagtt catgggcttt ccctcggccg aggcgagcgc
     2281 cggcgtgccg ctcatgtccc tgcgtggcgt ggccagcgtg atcgagatgg tcggcgggat
     2341 gctgctcttg ttcgggctgt tcacgcggcc catcgccttc atcctcgccg gggagatggc
     2401 ggtggcctac ttcatggtgc acgccccgcg cggattctgg cccctggtca atcgcgggga
     2461 accggccgtc ttcttctgct tcacgtggct gtacttcgcc gcggccggcg gcggcccgtg
     2521 gagcctggac gcgctacgcg gacgatccag cggacgagca tcgccctgac cggcacgtcc
     2581 gagaggacgt gaggggcgag gccgactacc gatcggcgtc ggccggattg ttcatggact
     2641 cgtgggcgac gtcgtggcct ttccggacct cgaagagtgc ggcgtctctg ccctgatcgg
     2701 cgcggttctg gtgacgatcc tgctggtcgt cggtcaacaa gggcgactac ttgggcgatg
     2761 accgtgatgg tatagtggcc gcggtttcgg gcgggagagc cggcggacgt cggcgctccg
     2821 cggcgagtat cgctccaccg catcatcgag aacggaggcc gaccatggga atgacgaagc
     2881 gaatcacggg acgctggcgt gtcgcagcca aactggccgt gctgatcggc gcgatcgtca
     2941 tcgcgtcgtc acagttcatc ggcgcgccca gggcggccga tccgatcaag atcggctggg
     3001 tcggcccgct gtcgcccccc ggcggctacg ccgagggcac gctcatgaag caggccgccc
     3061 agctcgcggc cgacgagatc aacgccaagg gtggcgtgct cggccggcag atccaggtgg
     3121 tcttcgccga cacgcgcggc aagccggagg aaggtaccgc ggcggcggag cggttgatct
     3181 cccaggagaa ggtcgtcgcc atcaccggcg agttccacag ctcggtgttc ctcgcggaga
     3241 tggaggttgc ccacaaggcg ggcatcccga tcatcgcggt ggacgtgtgg gcgctcagca
     3301 tcaccgccaa gggctacccg gaggtgttcc gggtcgcgcc caaccaggcg ctgatcgcca
     3361 cgaagtacgg cgactggatc gcggcggccg gcttcaagaa cgtggccttc ctcttcgaga
     3421 agaccgacgg cgggcagagc gcgcgcgacg tgctgctgcc ggtgctggac aagcacggcg
     3481 tcaagtacga ggcggtcggg gccgatccga actcgaccga gttcaccgcg cagatcgagc
     3541 gcttcaagac caagcagccg cccttcgact tcttcatgac cgcgtacagc gaggccggcg
     3601 cctatccgat gatcagccag tcgcacacgc tcggcttcgc gccgagcgcc aagacgggca
     3661 tcggcaactc gggcgggccg gcggttgatc cgacgttctg gaagaacgtg ggcgaggcgg
     3721 gcaagtacct ggtcaccgag atcgtgggcc tgcccaaggc ggcgtggaac gacaagacca
     3781 aggccttcgt ggcggcgtac aagaagaagt acaaccacac gccgtcgccg caggcgatcg
     3841 agaactacga cgcgatgctg gtgctgaccg acgcgatcgc ccgggccaag tccaccgacg
     3901 gcaaggcgct catcgcggca ctggagaaga ctgacatcgt gctgggccgg gggcgctaca
     3961 ccttctcgac cagcaagcag ccggactggg cctatcacca gttcatgggc gcgccggtgg
     4021 cgctggtgca gtacaacaag gtgggccagg acgccgagga cgcgccgatc gtctggccgc
     4081 gggacatcgc ggacgtcaag tacatgtacc tgaagccggg ccagtgagtc gccgctcgga
     4141 catataagaa gcgtttccgg gctaccagcg ggggaggccg tgcgccggcg cgtggatcgc
     4201 gccggcacgg gacctccccc gccgcgtccc tgagagcgca gtcaccagga tcaggcgatg
     4261 agctgggatt atctcttcgt ccaggccgcg aatggggcgg tcatcggcgt gatctacgcc
     4321 ctgatcgcgg tcggcgtgac gctcatcttc tccatcctca agatcgtcaa cttcgcccac
     4381 ggcgacctct acatgctcgg gggctactgc gcgtattacg tcatcgagta cctgggcttc
     4441 cccccgctga tcgcgatggt cgtggcgatg gcggcgctgt tcctgggggc gatcgtcctc
     4501 gagcggctca tgctgacgcc gctctacagc gacgccaccg agcgcaagga cgagtacggg
     4561 ctcatcgtca ccttcggcct cgcgtacttc ctgcgcaact cggcggtcat cgccttcggg
     4621 ccctttccgc tcaagccccc gtccttcatc ccgggggtgc agcggctggg caacctggtg
     4681 gtgaccaacg accggctggt cgcggccggg gtcggcatcg tgctgatcgc ggccatgctc
     4741 tacttcatga agcggacgat ctggggccag gcgctggacg cggtcagcca gtcgcgggac
     4801 tccgcggcga tcgtgggcat caacccgcgg cgcttcaact cgttcgcgtt cggcatcggg
     4861 gccgcgctcg ccgccgccgc gggcgccctg gtggcgccga tcttctcgct cgcgcccgac
     4921 atgggcgtgc tgcccaacat ccacgcctac gtgatcgtca tcctgggcgg catgggctcg
     4981 gtgagcggca gcatcatcgc gggcatcctg ctcggcgagt ccgagaacct cttcaccgcc
     5041 ttctttccgg atccgacgcg cgcgttcgcc tacacgaacg ccttcggggt cctggtgctg
     5101 atggtcgtcc tcgtcgtccg gccgcagggg ctcttcggcc gccgccacct gcggatggag
     5161 taggggaccg tgcgggggct gccggtctac atcctgctcg ccgtcgccgc gctggcgctc
     5221 gccggcgccg accgcctcaa cgcctactgg ctgcacgtgg cgatcatcgc gatgtactac
     5281 ggcatcctcg ccgcgagctg gtcgctgctc gccggctacg tgggcctgtt ctcgctgagc
     5341 cacaccgcgt tcgcgtcgat cgggggctac acctcggccc tgctggtgaa gtggctgggc
     5401 atcccggtgc tcgtgggcat gggcgccggc ctcgtgatgt gtggcgtgct cggcggggcg
     5461 atcggctggg tgtgcctgcg catgagcggg ccctacctcg cgatcttcac gctcgcgttc
     5521 tcggagatcg tgcgcatcac gatctcgacc gaggacgacg tgaccgcggg catggcgggc
     5581 ctgcacaccg ccccgctctt ccagagcgcg acccggctgc cctacttcta tgcggccttt
     5641 gccctgctcg cggtctcgct gctgggcatg gggctgctgg tgcgatcgcg gtggggcctc
     5701 ttcctgcgcg ccatccgcga ggacgagcag gcggccgggg cgtcgggggt ggccgtggcg
     5761 cgcttccgca tcctcgcgtt cgcgatcgcg agcggctttg ctgggctcgc cggcggcttc
     5821 tacgcgcact acatcggcat catgaccccg ggggtgggcg acctcgacga gatggccaag
     5881 ctggtggtca tgaccgtgat cggcggcatg gagcggctgc cgacggcggt cggcggcgcc
     5941 ttcgtgctgg aggtgctcct cgaggtgctc cggggcttcg gccagtggcg cctcccgctc
     6001 ttcggcgtgc tcctgctgct cacgatgcgc ttcgcccgca acgggctcct caccccgctg
     6061 tggcttccgt tcgtgcggct gggcgagacg cgggtgccgc cgctgcccgc cgcccgcgac
     6121 gtcccccaga aggactccgc cgcgtgagcc agggcgccga gctcgtccgg ggcgtggggc
     6181 tggtcaagcg gttcggcggc atcgtcgcca acgacgcggt ggacctcgtc gtcgcccagg
     6241 gcgacctcgt cgggctgatc ggcccgaacg gctccggcaa gaccacgctg atcaacgtgc
     6301 tgaccgggca cctcgatccc gacggcgggg aggtgctggt gcgcgggcgc cgggtgagcg
     6361 gccgcccgtc gcacgtctac gcggggatgg gcgtggcccg gacgttccag ctgacgcagc
     6421 tcttcggccg catgaccgtg ctcgagaaca tgctggtgcc cggcctgacg cgcccgcact
     6481 cccggtggga cgaggtcgcg cgccgcgccc gcggatacct ggagttcctc agcctgtccg
     6541 acgtcgcgca gctcccggcg aggaacctct cgggtgggca gcagaagctg ctcgagctcg
     6601 ggcgctgcct gatgctggag cccgcggtgc tgttcctgga cgagccgttc gccggcgtgc
     6661 atccgcggct gcgcgatgag ctcgtagcgc ggatccgcga gctccaccag ggcggccgca
     6721 cgttcgtggt cgtcgatcac gacatggagt cgatcctgcg catctcgcgg cgcctcgtgg
     6781 tcatggcgcg cggcagcaag atcgccgatg gcgagccggc ggtggtgcgg gcggatcaga
     6841 acgtgctcgc ggcctacgcg gggctctgag atgccggaca ccgtcgcgcc gtggctcgtg
     6901 gtgcgcaacc tc
//
LOCUS       DATMCV010000518         4208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_628204, whole genome shotgun sequence.
ACCESSION   DATMCV010000518 DATMCV010000000
VERSION     DATMCV010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628204"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1396
                     /locus_tag="VNU03_11965"
     CDS             173..1396
                     /locus_tag="VNU03_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWP75225.1"
                     /translation="MTRPTGPSGGRASGPPADPIAAVTHPDPYPYYRDLVARIPLYRD
                     EARGLWVAASADAVTAVLTSPLGRVRPAAEPVPTALVASPAADVFGRLARMTDGPGHA
                     TMKRAVSATLDSVDAPRALAHADRWARSLSAEIDPRSSPARVDEFALALSAHVIGSLL
                     AMPDEALPAAERCVGQYTGGVSPGADAAHVERGKAAAGHLIAMVRSLSTAPESGETAG
                     LLGVLHREAGRAGGADAPAVVANAIGFLTQAYEATAGLIGNTLLALAARPDLRARVER
                     APDLLTDAIREVLRYDPPVQNTRRFIVADGPVAGQRMTAGDTVLVVLASANHDAAANP
                     APERFDVSRASRRTFTLGAGIHGCPGESLAITIARAGIEQLLAHGVALAPLAEAVTYR
                     PSGNLRVPLFARGTA"
     gene            complement(1398..2507)
                     /locus_tag="VNU03_11970"
     CDS             complement(1398..2507)
                     /locus_tag="VNU03_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWP75226.1"
                     /translation="MKITDVTLTLFAWENIPATSYGRHTGKFGGQSQLGLLAIRTDDG
                     VEGHAFLGSASRGAQFDAESLVSALKPLVMDQDPLERERLYQALLSRNRHTTWRAIGA
                     VDVALWDIAGKIAGLPIHRLLGTYRDRLPAYASSAVLQSKQAYAEEAARFKASGWTAY
                     KIHPPTDPRVDIEICEAVRGMVGDAFTLMLDATWAYDYPAAIRVGRAIERLGYYWYED
                     PLADDDLYNYVKLKQHLSIPILATEYSPGGFTAYAPWIVARATDFLRGDVAVKGGISP
                     LMKAAHLAEGFHMNFEIHHGGNSLNNVANLHVAMAIRNCEFFEVLLPADAQKYGLVAD
                     IEVDSHGMVRAPTAPGLGAQIDFELIERKKLAVLR"
     gene            2597..2890
                     /locus_tag="VNU03_11975"
     CDS             2597..2890
                     /locus_tag="VNU03_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HWP75227.1"
                     /translation="MKPSPRSAPRRPAPPLPSDGVVRLFRDRGGRNGKTVTVIRGLPE
                     RGPALEDRVAQMKRICGAGGTLKDGTVEIQGDHRERLAEHLRSLGYTVKLAGG"
     gene            complement(2887..3171)
                     /locus_tag="VNU03_11980"
     CDS             complement(2887..3171)
                     /locus_tag="VNU03_11980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWP75228.1"
                     /translation="MLGIVATIKVKTGMEQQFEAVAKELVAKVNAGEPGCRLYALHKA
                     EAPLTYVFMERYADQAAVEAHRGTDYFKALGRKMGEFMDGRPEVLRLAEI"
     gene            3380..3763
                     /locus_tag="VNU03_11985"
     CDS             3380..3763
                     /locus_tag="VNU03_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75229.1"
                     /translation="MHGTREAAARQHINDPHPPEVRRRRGRPKGSTGISRRHPDSLLA
                     AEIAKSPLATVYASADGELYHAVCKTPLEFQGRRAQLEFDFYCLRCMEHVALPQFALS
                     RIRFDLPRPADATLPDWATSIVAYQ"
     gene            complement(3765..3992)
                     /locus_tag="VNU03_11990"
     CDS             complement(3765..3992)
                     /locus_tag="VNU03_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWP75230.1"
                     /translation="MFITVVLTDACVESSARHAAERGFQVIVVDDACAAWEEAFHAAS
                     LRSLSRYFARIASTGEVLEGIERAAGAGGAR"
BASE COUNT          587 a         1549 c         1452 g          620 t
ORIGIN      
        1 ccaggtcgct gaccgtcgcc tcgcccgcca gcagggcggc caggatcgcg agccgcgacg
       61 gctctcccag ggcacccgcc acccgcgcca cgtcttgcat cgctcccatc gcgagggtag
      121 tataccttcc gttcgtgtat atatctcctg atcgagatat atggaggcac acatgaccag
      181 accgaccggc ccgagcggcg gccgcgcgtc ggggccgccg gccgacccga tcgcggcggt
      241 cacgcacccg gatccctacc cgtactatcg cgacctggtc gcccgcatcc cgctctaccg
      301 ggacgaggcc cggggcctgt gggtggccgc gagcgcggac gcggtgaccg ccgtgctcac
      361 gagtccgctc gggcgcgttc gccccgccgc ggagcccgtg cccaccgccc tcgtcgcctc
      421 accggcggcc gacgtcttcg gccggctcgc acgcatgacc gacggtcccg gccacgcgac
      481 gatgaagcgg gcggtgtcgg ctacgctgga ctcggtcgat gctccccgcg ccctcgcgca
      541 cgccgaccgc tgggcccgga gcctctcggc ggagatcgac ccgcgctcga gcccggcgcg
      601 ggtggacgag ttcgcgctcg cgctctcggc gcacgtcatc ggcagcctcc tcgcgatgcc
      661 cgacgaggcg ctgccggcgg cggagcggtg cgtcggccag tacaccggcg gcgtctcgcc
      721 cggcgccgac gcggctcacg tcgagcgggg caaggcggcg gcggggcact tgatcgcgat
      781 ggtccggtcg ctctcgaccg cgcccgagtc cggcgagacg gcggggctgc tcggcgtcct
      841 gcatcgggag gcgggccggg ccgggggcgc cgacgcgccg gcggtcgtgg cgaatgcgat
      901 cggcttcctg acccaggcct acgaggcgac cgcgggcctg atcggcaata cactcctggc
      961 cctcgcggcc cgtcccgacc tccgcgctcg ggtggagcgc gcccccgatc tcctgaccga
     1021 cgcgatccgg gaggtgctcc gctacgaccc gccggtgcag aacacgcgcc gcttcatcgt
     1081 ggcggacggc ccggtggcgg gccagcggat gacggcgggc gacacggtcc tggtcgtgct
     1141 ggcgtcggcc aaccacgacg cggcggcgaa tccggctccg gagcgattcg acgtctcgcg
     1201 cgcgagccgg cggacgttca cgctcggcgc cgggatccac ggctgtccgg gagagtcgct
     1261 cgcgatcacg atcgcccggg ccggaatcga gcagctactc gcccacggcg tcgccctcgc
     1321 ccccctcgcc gaggcggtga cctatcgccc ctccggcaat ctgcgggtcc cgctcttcgc
     1381 ccgggggacc gcctgagcta ccgcagcacc gccagcttct tgcgctcgat cagctcgaag
     1441 tcgatctggg cgcccaggcc cggcgcggtg ggggcccgca ccatgccgtg cgagtcgacc
     1501 tcgatgtcgg cgaccaggcc gtacttctgc gcgtcggcgg gcaggagcac ctcgaagaac
     1561 tcgcagttcc ggatggccat cgccacgtgc aggttggcca cgttgttgag cgagttgccc
     1621 ccgtggtgga tctcgaagtt catgtggaac ccctcggcca ggtgcgccgc cttcatcagc
     1681 ggcgagatgc cacccttgac cgccacgtcg ccccggagga agtcggtcgc gcgggccacg
     1741 atccagggcg cgtaggcggt gaagccgccc ggcgagtact cggtggccag gatcgggatc
     1801 gagaggtgct gcttgagctt gacgtagttg tagaggtcgt cgtcggccag cgggtcctcg
     1861 taccagtagt agccgagccg ctcgatggcg cggccgaccc ggatcgcggc cgggtagtcg
     1921 taggcccagg tggcatcgag catgagggtg aaggcgtcgc ccaccatccc gcggaccgcc
     1981 tcgcagatct cgatgtcgac ccgcgggtcc gtgggcggat ggatcttgta cgcggtccag
     2041 cccgaggcct tgaagcgcgc cgcttcttcc gcgtaggcct gcttggactg gagcaccgcc
     2101 gagctcgcgt aggcgggcag gcggtcccgg taggtgccga gcagccggtg aatcggcagc
     2161 cccgcgatct tgcccgcgat gtcccacagg gccacgtcga ccgcgccgat ggcgcgccac
     2221 gtggtgtgcc ggttgcggga cagcagcgcc tggtagaggc gctcgcgctc gagcgggtcc
     2281 tggtccatca cgaggggctt gagcgcgctc accagcgact ccgcgtcgaa ctgcgcgccg
     2341 cggctggccg agcccaggaa ggcgtggcct tcgacgccgt cgtcggtgcg gatcgcgagc
     2401 aggccgagct gactctggcc gccgaacttt cccgtgtgcc ggccgtagga cgtggccgga
     2461 atgttctccc acgcgaacag ggttagcgtg acgtcggtga tcttcacgag gctccttccc
     2521 gcccgcgagc cgggctatcg acgctggcca gaagcgtaca ccgattcctg caccgggatt
     2581 ctgctagatt cggtccgtga agccgagccc gcgttccgcg ccccggcgtc cggcgccgcc
     2641 gctgccgagc gacggggtcg tccgtctctt ccgggatcgc ggcggccgca acggcaagac
     2701 ggtcaccgtg atccgcgggc tgccggagcg cgggcccgcc ctcgaggacc gcgtcgccca
     2761 gatgaagcgg atctgtggcg cgggcggcac gctcaaggac gggacggtcg agatccaggg
     2821 cgatcaccgc gagcgcctcg ccgagcacct ccggtccctg ggctacaccg tgaagctcgc
     2881 ggggggctag atctcggcga gccgcaggac ttccgggcgc ccatccatga actcgcccat
     2941 cttccgaccg agcgccttga agtagtcggt gccgcggtgg gcctccaccg cggcctgatc
     3001 cgcgtaacgc tccatgaaga cgtaggtgag cggcgcctcg gctttgtgca gagcgtagag
     3061 gcggcaaccc ggctcgccgg cgttgacctt ggcgaccaac tccttggcca cggcctcgaa
     3121 ctgctgctcc attcccgtct tcaccttgat cgtcgcgacg atgcccagca tcgggagcct
     3181 ccgggtggga cacccggcgt gggcgcccac tgttcgtggg ttgacaacct ccggctacga
     3241 gagaaccaaa atttgtagcg agcgccccgt gttcaagggg tttacggcta actccttcaa
     3301 ctagatcaac tacttgcaga cagtgtcacg cctggaacgc gtcttgctcc taggcccggg
     3361 catgagaact caagatcgcg tgcacgggac ccgggaagcc gcagccaggc agcacatcaa
     3421 cgacccgcat ccgcccgagg tccgacgccg ccggggccgg cccaagggta gcaccggcat
     3481 cagccggcgc caccccgact cgctcctggc cgcggagatc gccaagtcac ccctggccac
     3541 ggtctacgcg agcgcggacg gcgagctcta ccacgcggtc tgcaagaccc cgctcgagtt
     3601 ccagggccgt cgggcccagc tggagttcga cttctactgc ctgcgctgca tggagcacgt
     3661 cgcgctgccg cagttcgcgc tgagccgcat ccgcttcgat ctgccgcggc cggccgacgc
     3721 cacgctgccg gactgggcca ccagcatcgt cgcgtaccag tagctcaccg cgccccgccg
     3781 gctcccgccg cccgctcgat cccttccagg acctctccgg tcgaggcgat ccgggcgaag
     3841 taccgcgaca ggctcctcag gctggccgcg tggaaggcct cctcccacgc ggcgcacgcg
     3901 tcgtcgacca cgatcacctg aaatccccgc tccgccgcgt gccgggccga gctctccacg
     3961 cacgcatcgg tcagcaccac ggtgatgaac aaccgacctc tccgttgtca agggcgggag
     4021 cccgtgccag catgcgccgg tggtcatcga agatcccgac gacccgcgag tcgccgacta
     4081 ccgcgcggcg ggacgcggcg gagccgacgg gctgttcctc tgcgagggcc gcctcgtggt
     4141 gcgccggctg ctcggcggct cccgctaccg ggtgcgctcg gtcctcgcca ccgcgccggt
     4201 cctcgaag
//
LOCUS       DATMCV010000519         2613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_95453, whole genome shotgun sequence.
ACCESSION   DATMCV010000519 DATMCV010000000
VERSION     DATMCV010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95453"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..2309)
                     /locus_tag="VNU03_11995"
     CDS             complement(99..2309)
                     /locus_tag="VNU03_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP75231.1"
                     /translation="MRGPIVYEAPRPAAAPGETVVTSTCGHNCGGRCVVNAHVRDGRI
                     VRISTDPRKWTPEVAPLHACVRGFGQLERLNHPDRVVYPLRRVGPRGGGRFERIGWDE
                     ALDEVAGQMRRIRDTYGPAAILDCSRTGSLSMLHSRSTVKRLLYMFGGCTELWTNISA
                     EAEVYAVRQTYGAKADYKSAGREPTDYVNSRLILMWGWSPADGTFGTGTLQYLKLAKR
                     RGVKIICVDPRRTRTSWELADQHVFIRPSTDTAALLAMAHVIVTEGLHDQAYLDRHVL
                     GFDEAHLPAGAPAGASYRAYLLGEADGVPKTPEWAAAITGVPAETLRALAVEYATSKP
                     AALQTGYAPGRTLHGEQFHRAAYALCAMTGNVGIPGGNAGTSNGATGRGGIQGLPTGD
                     NPMGRGVSSPLLADLLERGKAGGYPADVKMIYSCAGDLFNQLPNVRRIIAGVERLDFV
                     VVQDHFVTPTARYADIVLPATTFWERNDVHTPWAGAGHYAIFMQQAIAPVGECRNDLD
                     ICAALADRLGIVGYNDKTEDEWLRELTRHAIDDYETFRAKGLARLPAPEDAVAFAREI
                     REPERHPFSTPSGKIEIYSMAIAANPDPYGLGAIPPIPTFIPEPVDARHPLRLVTPKS
                     RARTHSIHDNQAILSRADRDDVWLHPADAAARGIVDGQRVAVFNDRGRTLIPARVTDR
                     VAPGVVSIKEGAWFALDESGQDTRGCSNLLTPDRSSPAGATPFNSCFVEIEAAP"
     gene            2381..>2613
                     /locus_tag="VNU03_12000"
     CDS             2381..>2613
                     /locus_tag="VNU03_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP75232.1"
                     /translation="MARRIVIVGGGLMGLGAAFHLRRADPGAEVTVLERERVGAAASG
                     ASAAGVRVMGRDPAERALALASLARWPDLDREL"
BASE COUNT          317 a          893 c         1021 g          382 t
ORIGIN      
        1 ggccccgggc tacgagccca ccatcgtgta tgtgcgggtg gtgcgcgact ggaccacccg
       61 cgtccgcctg accctgatcc cggcccgctg agccggggct agggggcggc ttcgatctcc
      121 acgaagcagg agttgaaggg cgtggccccg gcgggtgagg agcgatcggg ggtcaggagg
      181 ttcgagcagc cgcgcgtgtc ctggccggac tcatcgaggg cgaaccacgc gccctccttg
      241 atcgacacca cgccgggggc cacccggtcg gtgacccgcg ccgggatcag ggtgcgcccg
      301 cggtcgttga agaccgccac gcgctgcccg tccacgatgc cgcgcgccgc cgcgtcggcc
      361 gggtgcagcc acacgtcgtc gcgatcggcg cgagacagga tggcctggtt gtcgtggatc
      421 gagtgggtgc gggcgcgcga cttgggggtg acgagccgca gcggatggcg cgcgtcgacc
      481 ggctccggga tgaaggtcgg gatcgggggg atcgcgccga ggccgtaggg atcggggttg
      541 gccgcgatgg ccatcgagta gatctcgatc ttgccggacg gggtgctgaa ggggtggcgc
      601 tccggctcgc ggatctcgcg tgcgaaggcc accgcgtcct ccggggcggg caggcgggcc
      661 aggcccttgg cccggaacgt ctcgtagtcg tcgatggcgt gccgggtcag ctcgcgcagc
      721 cactcgtcct cggtcttgtc gttgtagccc acgatgccga ggcggtcggc cagcgccgcg
      781 cagatgtcga ggtcgttgcg gcactcgccc accggggcga tcgcctgctg catgaagatc
      841 gcgtagtggc cggcgcccgc ccacggggtg tgcacgtcgt tgcgctccca gaaggtcgtc
      901 gcgggcagca cgatgtcggc gtaacgcgcg gtcggggtca cgaagtggtc ctggaccacc
      961 acgaagtcga gccgctccac gcccgcgatg atccgccgca cgttggggag ctggttgaag
     1021 agatcgcccg cgcacgagta gatcatcttc acgtcggccg ggtagccgcc cgccttgccg
     1081 cgctcgagca gatcggccag gagcggggag gacacgcccc ggcccatcgg attgtcgccg
     1141 gtgggcaggc cctggatgcc gccgcgcccg gtcgccccgt tgctggtgcc cgcgttgccg
     1201 cccgggatgc ccacgttgcc ggtcatcgcg cagagcgcgt aggccgcgcg gtggaactgc
     1261 tccccgtgga gcgtgcgccc cggcgcgtag ccggtctgga gcgcggccgg cttgctcgtc
     1321 gcgtactcga ccgcgagcgc gcgcagggtc tcggccggca ccccggtgat ggcggcggcc
     1381 cactcgggcg tcttcggcac cccgtcggcc tcgccgagca ggtacgcacg gtaggatgcg
     1441 ccggccggcg cgcccgcggg caggtgcgcc tcgtcgaagc cgaggacgtg gcggtccagg
     1501 taggcctggt cgtgcaggcc ctcggtcacg atcacgtggg ccatcgcgag cagcgccgcg
     1561 gtgtcggtcg acgggcggat gaacacgtgc tggtcggcca gctcccagct cgtgcgcgtg
     1621 cgacgcggat ccacgcagat gatcttgacc ccgcgccgct tggccagctt caggtactgc
     1681 agcgtgccgg tgccgaaggt gccgtcggcc gggctccagc cccacatgag gatcaggcgc
     1741 gagttgacgt agtcggtggg ctcgcggccc gcgctcttgt agtcggcctt ggccccgtag
     1801 gtctggcgga ccgcgtagac ctccgcctcc gccgagatgt tggtccacag ctcggtgcag
     1861 ccgccgaaca tgtagagcag gcgcttgacg gtggagcggc tgtgcagcat cgacaggctg
     1921 ccggtgcgcg agcagtccag gatggcggcc gggccgtacg tgtcgcggat gcgccgcatc
     1981 tggccggcca cctcgtcgag ggcctcgtcc cagccgatgc gctcgaaccg tccgccgccg
     2041 cgcgggccca cgcgccgcag cggatagacg acgcggtccg ggtgattcag ccgctcgagc
     2101 tggccgaagc cgcgcacgca ggcgtggagc ggcgcgacct cgggcgtcca cttgcgcgga
     2161 tcggtgctga tccgcacgat gcgcccgtcg cgcacgtggg cgttgaccac gcagcgcccg
     2221 ccgcagttgt ggccgcaggt gctggtgacc acggtctcgc cgggtgcggc ggcgggtcgc
     2281 ggggcttcgt agacgatggg gccgcgcatg gggtctgccg gagactctat cacctcccgc
     2341 cgggtcgccg gtaggccgcc cggtgctaga ctgcggcgtc atggcgcggc ggatcgtcat
     2401 cgtgggcggc gggctgatgg ggctgggcgc ggcgttccat ctccgccgcg cggatccggg
     2461 cgcggaggtc accgtgctcg agcgcgagcg cgtgggcgcg gcggcctcgg gggccagcgc
     2521 cgccggcgtg cgggtgatgg gccgcgatcc ggccgagcgc gcgctggcgc tcgcgagcct
     2581 cgcccgctgg cccgatctcg accgcgagct gga
//
LOCUS       DATMCV010000520         3647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_95458, whole genome shotgun sequence.
ACCESSION   DATMCV010000520 DATMCV010000000
VERSION     DATMCV010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95458"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1162
                     /locus_tag="VNU03_12005"
     CDS             146..1162
                     /locus_tag="VNU03_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWP75233.1"
                     /translation="MARSTARLATVVMAFLLVAAPGHYQIARAQVVTWGMANEYPATS
                     IQGEADARFAREVTRRSGGRIEIAHQYDASSGFRSKEMVDAIGRGELRIGNTYMGALG
                     GLDPVFLLPSLPFLATTPEQGQALAAVARPAYERALARHNQILLFQSPWPAAGIWANR
                     PVDSVEALRGLRVRTADANGVLAFRAAGAVPVVVSFADALPQLRSGQLDAVLSSGDGG
                     AGQRLMETLRHFTAIEYAVTMSMVTINADAWRALDPARQQAVREAAEETEARQWEVMR
                     TRVAANYAQMRAAGITLTTELSSDYRRALRAAGQVAVDDWLRQMGPAGAQILEAYRAR
                     LAGR"
     gene            complement(1168..1641)
                     /locus_tag="VNU03_12010"
     CDS             complement(1168..1641)
                     /locus_tag="VNU03_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP75234.1"
                     /translation="MAAIRGRVERRLAELGLELPHQPVPVGNYLPAARVGNLVFTSGQ
                     TARINGVRRYVGVVGQEVSEADAYLSARDAILNCLACVKQAVGSLDRVTRVVKVIGFV
                     NVGQDFVTHPTVINGASDLLQQLFGEAGRHARSAVGLSSLPSNVSVEVELVVEVR"
     gene            complement(1646..2881)
                     /locus_tag="VNU03_12015"
     CDS             complement(1646..2881)
                     /locus_tag="VNU03_12015"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWP75235.1"
                     /translation="MYFTGFDCLGCHASYPPDQDLLLCPACHDLLEARYDLARFGREV
                     DRDALARRPSDVWRWTELLPVLDPTRIVTLGEGGTPFLRSERLARACGVRELWLKSDA
                     ANPTGSLKDRSITVSATKAVEFGYGVLACDSTGNKASSTAAYAARAGLQSVVFCPKDT
                     PRPKMAQAVFFGARLIRVDGHYSQINAMYRRLIASGRVKWYDCGTDNPFRYEGKKTYA
                     YEIAQDLGWRVPDRVLHPAAGGMSLVKTWKGFNELRTLGWVDRLPKMTVVQAARCAPI
                     VLSWTQGLPRVAAVEKQATAASALAAADPGLLGDRALHTVRASGGSGIAVDETEILDA
                     VRLLGQDGLMIEPSGAVTVAAVRRGVREGSIDPGESVVCVLTGSGFKDFDRILDLVEI
                     PTQVIAGYEAMVAEAERIG"
     gene            complement(2913..>3647)
                     /locus_tag="VNU03_12020"
     CDS             complement(2913..>3647)
                     /locus_tag="VNU03_12020"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75236.1"
                     /translation="GVLLFLVVPVLSVIPMSFSSSMVFELIPSEPSLTQYRRFFSSPE
                     WMAALGRSVQVALGTTFVATIMGALAALGLRRLRGTRRAVLEALLIAPRIVPTIVLAV
                     ALYFVFSQTGLVGTLGGLVLAHSVLAFPFVVVLVGSALQGLDPALEEASRSLGGNPLR
                     TFCRVTFPQIRLAVLGSALFSFNVSFDEVVITLFVSGVRTKTLPVKVWDAILYEITPI
                     LPAISAVIIVASVLLLTPLLLARRSS"
BASE COUNT          559 a         1301 c         1331 g          456 t
ORIGIN      
        1 cctcgcccgg tccgggctcg ggcaccggga ggccggccac gaccaatcgc cggtcccgca
       61 tcacggcggc acgcatgggc gcgatcctac tccgtcccgc gcccaccgtc gagaggtgcg
      121 ctatcctcgg gccggaggca cccggatggc tcgatcgacg gcgcggctcg cgacggtggt
      181 gatggcattt ctgctggtgg ccgcgcccgg ccactaccag atcgcccgcg cccaggtcgt
      241 cacgtggggc atggccaacg agtacccggc cacctcgatc cagggcgagg ccgacgcgcg
      301 cttcgcccgc gaggtcacgc ggcgctccgg cgggcgcatc gagatcgcgc accagtacga
      361 cgcctcgagc ggcttccgct cgaaggagat ggtcgacgcg atcgggcgcg gcgagctgcg
      421 gatcggcaac acctacatgg gcgctctggg cggtctcgat ccggtgttcc tcctcccgtc
      481 gctgccgttc ctggccacca cccccgagca gggccaggcc ctcgccgcgg tcgcgcggcc
      541 ggcctacgag cgcgcgctcg cccgccacaa ccagatcctc ctcttccagt cgccctggcc
      601 ggccgcgggg atctgggcca accggccggt ggactcggtg gaggcgctcc ggggcctgcg
      661 cgtccgcacc gccgacgcca acggggtgct cgcgttccgc gccgccggcg cggtcccggt
      721 ggtggtgtcc ttcgccgacg cgctgcccca gctccgctcg ggccagctcg acgcggtcct
      781 ctcctcgggc gacggcgggg cggggcagcg gctcatggag acgctgcggc acttcaccgc
      841 catcgagtac gcggtgacca tgagcatggt cacgatcaac gccgacgcct ggcgcgcgct
      901 cgatccggcg cggcagcagg cggtgcgcga ggcggcggag gagaccgagg cccggcagtg
      961 ggaggtgatg aggacgcgcg tggcggccaa ctacgcccag atgcgcgcgg ccggcatcac
     1021 gctgaccacc gagctgtcgt ccgactaccg ccgggcgctc cgcgcggcgg ggcaggtcgc
     1081 ggtggacgac tggctgcgcc agatgggccc ggcgggcgcg cagatcctcg aggcgtaccg
     1141 ggcgcggctc gccggccgct gacccgctca gcgcacctcg acgaccagct ccacctcgac
     1201 cgagacgttc gagggcaggg aggagaggcc gaccgcggag cgcgcgtggc gccccgcctc
     1261 gccgaagagc tgctggagca ggtccgaggc cccgttgatg acggtggggt gggtgacgaa
     1321 gtcctgcccc acgttcacga agccgatcac cttcaccacg cgagtcaccc ggtccagcga
     1381 gccgaccgcc tgcttcacac aggccaggca gttgagtatc gcgtcgcgcg ccgagagata
     1441 ggcgtccgcc tcgctcacct cctggccgac cacccccacg tagcggcgca ccccgttgat
     1501 gcgcgcggtc tgcccggagg tgaacaccag gttgcccacc cgcgcggcgg gcaggtagtt
     1561 gccgaccggc accggctggt gcgggagctc gagccccagc tcggcgaggc gccgctcgac
     1621 gcgacctctg atcgcggcca tgcggtcagc cgatccgctc ggcttcggcc accatcgcct
     1681 cgtagccggc gatcacctgc gtcgggatct cgaccagatc caggatgcgg tcgaagtcct
     1741 tgaagccgga gccggtgagc acgcagacca ccgactcgcc gggatcgatg gatccctcgc
     1801 ggacgccgcg gcgcaccgcg gccaccgtga ccgcgccgct cggctcgatc atgaggccgt
     1861 cctgaccgag caggcggacc gcgtccagga tctccgtctc gtcgaccgcg atcccggagc
     1921 cgccggaggc ccggacggtg tgaagggccc ggtcgccgag caggccggga tcggcggcgg
     1981 ccagcgcgct cgccgcggtg gcctgcttct cgaccgcggc cacccgcggc aggccctggg
     2041 tccacgatag gacgatgggc gcgcagcggg cggcctgcac caccgtcatc ttcgggagcc
     2101 gatccaccca gccgagcgtg cgcagctcgt tgaagccctt ccacgtcttc acgagggaca
     2161 tgccgccagc ggccggatgc agcacccggt ccggcacgcg ccagccgaga tcctgggcga
     2221 tctcgtaggc gtaagtcttc ttgccctcgt agcggaacgg gttatcggtg ccgcagtcgt
     2281 accacttcac ccggccactc gcgatgagcc gccggtacat cgcgttgatc tgcgagtagt
     2341 ggccgtccac ccggatgagc cgcgccccga agaacaccgc ctgcgccatc ttgggtcgcg
     2401 gcgtgtcctt cgggcagaac accacgctct ggagccccgc gcgcgcggcg taggccgcgg
     2461 tcgacgaggc cttgttgccg gtcgagtcgc aggcgagcac gccgtagccg aactcgaccg
     2521 ccttggtcgc ggacacggtg atggagcggt ccttgagcga gccggtgggg ttggccgcgt
     2581 cggacttgag ccagagctcg cgcaccccgc acgcgcgggc caggcgctcg gaccggagga
     2641 agggcgtgcc gccctcgccg agcgtcacga tgcgcgtcgg gtccagcacc ggcagcagct
     2701 ccgtccagcg ccagacgtcg gagggccggc gggcgagggc gtcccgatcc acctcccggc
     2761 cgaagcgggc gagatcgtag cgcgcctcga gcaggtcgtg gcacgcagga cacagcagca
     2821 ggtcctggtc gggcggatac gacgcgtggc agccgagaca gtcgaagccg gtgaagtaca
     2881 tggcgcgcct catgatagcg ctgcgcggcc ggtcatgacg accggcgcgc gagcaggagc
     2941 ggggtgagca ggagcacgct cgccacgatg atcaccgccg agatggcggg caggatcggg
     3001 gtgatctcgt agaggatcgc gtcccagacc ttcaccggca gggtcttggt ccggactccg
     3061 ctgacgaaga gggtgatgac gacctcgtcg aacgacacgt tgaaggagaa gagcgcgctc
     3121 cccagcaccg cgagccggat ctgggggaag gtcacgcggc agaaggtgcg gagcggattg
     3181 ccgccgaggc tccgggaggc ttcctcgagg gcgggatcga gcccctggag cgcgctcccg
     3241 accagcacca ccacgaacgg aaaggcgagc acgctgtggg cgaggacgag gccgccgagg
     3301 gtgccgacca ggccggtctg ggagaagacg aagtagagcg ccaccgcgag cacgatggtc
     3361 ggcacgatgc gcggggcgat gagcagcgcc tcgagcaccg cgcgccgcgt cccccgcagg
     3421 cgtcggagcc cgagggccgc gagggcaccc atgatggtgg cgacgaaggt ggtgccgagg
     3481 gccacctgga cggagcgccc gagcgcggcc atccactccg ggctcgagaa gaagcgccgg
     3541 tactgggtga ggctcggctc gctcgggatc agctcgaaga ccatcgacga gctgaaggac
     3601 atggggatca ccgagagcac cggcaccacc aggaagagca gcacgcc
//
LOCUS       DATMCV010000521         6576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_430334, whole genome shotgun sequence.
ACCESSION   DATMCV010000521 DATMCV010000000
VERSION     DATMCV010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430334"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VNU03_12025"
     CDS             <1..219
                     /locus_tag="VNU03_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWP75237.1"
                     /translation="DTFPGQVFHGTVDSLSAGTGSRFSLLPPENATGNWVKVVQRVPV
                     KIRLDPREFGNPHTLRAGMSAYVVIRVR"
     gene            242..1849
                     /locus_tag="VNU03_12030"
     CDS             242..1849
                     /locus_tag="VNU03_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWP75238.1"
                     /translation="MTPDAGPISSARKWAITLSVMVVAFMQVLDTSVTNVVLPHLQGS
                     LSAGLEEVSWVITSYLAANAIIIPATGWLAGVLGRKRFFLICCVLFTVSSVLSGMAPN
                     LTFLILARIFQGIGGGPIIPLSQAILWEIFPLGQRGLAMAVWGVGFMMGPIIGPTLGG
                     YIADNWSWRWIFYINLPVGLLGFFMASAFLFDPPYLRKPARVDWPGLVLMVLGFGCLQ
                     LFLDRGEREDWFDSTLIVTLAIVAGVALVGFVARELLAKEPVLDLAVFTDRNFAFGAT
                     FIALIGLGMFSSMVLLALYSQKLLGYDALTSGLVLAPGGVGNMFSLILCGRLVTRVDQ
                     RVLLTFGCLVNALSLYMMSNLTLGMDYWTLTLPRVIQGFALGFIFVPLSTLTLATIRR
                     DKLVNATSAYNVLRNLGGSVGIALATTLLAQRSQFHQQNLVSHVTVWDPATQERLARW
                     TSHFLAHGSDAFTAQRQALAMLYQDAVGQAQVLAYADDFWLLAILFSIVPLFLPLMRR
                     VRVDAVSPSEEAEGEGGRVPPLPAPVD"
     gene            complement(1846..2253)
                     /locus_tag="VNU03_12035"
     CDS             complement(1846..2253)
                     /locus_tag="VNU03_12035"
                     /inference="COORDINATES: protein motif:HMM:NF017277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HWP75239.1"
                     /translation="MIQPAPRPIALLDHDPRRAIQDETIVCLICGRALRQLTNTHLRS
                     HGMGPDAYRREFGYNARRSLMCHRLRRFYAERAVRSNLAGRIRQRPIVLQPELRRRGG
                     RRSLAWEEVLTRFEARHPGKGARPRPVTAAAAD"
     gene            2450..6460
                     /locus_tag="VNU03_12040"
     CDS             2450..6460
                     /locus_tag="VNU03_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWP75240.1"
                     /translation="MGGLAGLIGNSPLMLALRAQVERLLARPSPAGGRLPPLLLLGET
                     GVGKGLLVRLMHEASVRRERPLIELNCAAIPESLVEAELFGHERGAFTDARQSRPGLF
                     QAAHGGLLFLDEVNSLPLATQAKLLTAIEQREVRRLGSTRADPVDAWIVSATNTRLDA
                     AVARREFRLDLYHRISAVILEMPPLRARGRDVLALAETFLVRSCAEYGLEPKRLGTAA
                     EGALLAHPWPGNVRELRNAIERAVLLADGDEITPELMELPRPRSHAAASPRTRPEGGD
                     ERESLRDTLEAAGWNLSRAAARLGIPRNTLRYRIERLGLRPPAVDVAAPEPAGPPPAP
                     ATELRWEKRWIAAVQVRLALPPARSSYHLTPLLEELIEKARSFGGRLDALHPLGFTAL
                     YGIEPMEAAPTRAALAAQAIVNAAAGYREADGERAGAGCRVHAAECLIARGAPIVGMS
                     VEDRRRLDEALDAIGMGPGLIAVSAEAVPFLERRFIVEPEVAGTVTTYRLAGPGRSGF
                     DVGGQQPSPFVGRARELARLRDLLAAAERGRGQVVDVMGEPGVGKSRLLHEFRRTVGP
                     DRVLYLEGRCTAHGASTPYLPLVHLLQQCFGFTEADGSEAIRVRVREGVARLGLSAGT
                     VAPYLLHLLGVPGRPGDGVAGSSPESVRARTVDALRYVMIAASQQRPVILAIEDLQWM
                     DPTSVASLAVLAPSVAASRILVLTTCRRGHRPPWLGRAHASQIDLDRLGPAESLTIVQ
                     ALLAGREVAAGVADALVSKADGIPFFLEELARVIVADPASRVPGIPDTIHEAIAARVD
                     RLAPADRDLLQGAAVLGRDVPRALLREMSGLADPEFGIALSRLRVTGFLRETAADPET
                     CGFTHVLTQETAYRSLTAERRRALHEAAAAAIEKRQPEIADRHPEVLAHHLTEAGATT
                     AAAYWHRAGRLAIRRSANAEALAHLRRALVLLEAQPPGAERARAELGLQLTVGAALVA
                     DRGYGARELDLTMGRLEALIGEVVDAPEVPVARFALCRFSIARADLVAAGRFGQEMLT
                     AAGSDPEAVIPASVAAGITDFYLGRFLRAREHFERVLAVHEPRFAEAQIARYGNDMRV
                     AALGFLGWTCALTGELDRAARLAEECVTRARESRHPLTLAVALNWAGLVRCERHEPAQ
                     AARLGEELLDLSREQSFAFFAALALGLLGWARLARGERAAGAELLRQGFDRYRAANQR
                     VGLRPRAHHVEGLLSLGAVDEALERLDEFIAHSRDTGERGLVSELFRLKGEALARRAP
                     ADPEATRALEQAVAIAQEQGATLLALRAAIGLVQVERERGDATPSLAALRRAYQGMTE
                     GSDHPHMQAARALLGE"
BASE COUNT          792 a         2435 c         2391 g          958 t
ORIGIN      
        1 gacacgttcc ccggccaggt cttccacgga accgtcgatt cgctcagcgc gggcaccggc
       61 tcgcgcttct cgctgctgcc gcccgagaac gcgaccggga actgggtgaa ggtggtccag
      121 cgcgtgccgg tgaagatccg actggatccc cgcgagttcg gcaatcccca caccctgcgg
      181 gccgggatgt cggcgtacgt cgtcatccgg gtgcggtagc gaagcgagcg gcgcggtggc
      241 cgtgaccccc gacgccggcc cgatctcgtc ggcccggaaa tgggccatca ctctctcggt
      301 gatggtggtg gccttcatgc aggtgctcga caccagcgtg accaacgtgg tgctgccgca
      361 tctgcaaggc tcgctctccg ccgggctcga ggaggtgtcg tgggtcatca cctcctacct
      421 cgcggccaac gccatcatca ttcccgccac cggctggctc gccggggtgc tgggccgcaa
      481 gcgcttcttc ctgatctgct gcgtgctgtt cacggtgagc tcagtgctgt ccggcatggc
      541 ccccaacctg accttcctga tcctcgcgcg catcttccag ggcatcgggg gcgggccgat
      601 catcccgctc tcgcaggcca tcctgtggga gatcttcccg ctcgggcagc gcggcctcgc
      661 gatggcggtg tggggcgtcg ggttcatgat gggaccgatc atcggcccga ccctcggggg
      721 ctacatcgcg gacaactggt cctggcgctg gatcttctac atcaacctgc cggtcggcct
      781 gctcggcttc ttcatggcga gcgcgttcct cttcgacccg ccctacctgc gcaagccggc
      841 gcgggtggac tggcccgggc tcgtgctcat ggtcctgggc ttcggctgcc tgcagctctt
      901 cctcgaccgg ggcgagcgcg aggactggtt cgactcgacc ctgatcgtca ccctcgcgat
      961 cgtggccggc gtcgccctgg tgggcttcgt ggcccgggag ctgctggcga aggaaccggt
     1021 cctcgacctg gccgtgttca ccgaccggaa cttcgcgttc ggggccacct tcatcgccct
     1081 catcgggctc ggcatgttct cgagcatggt gctgctcgcg ctctactccc agaagctgct
     1141 cggctacgac gcgctgacct cgggcctggt gctcgcgccg gggggcgtcg gcaacatgtt
     1201 ctcgctcatc ctgtgcgggc ggctggtgac gcgcgtcgac cagcgcgtgc tcctcacctt
     1261 cggctgcctc gtcaacgcgc tgagcctcta catgatgtcg aacctgaccc tggggatgga
     1321 ctactggacg ctcaccctcc cgcgggtgat ccagggcttc gcgctcggct tcatcttcgt
     1381 gccgctctcc acgctgacgc tcgccaccat ccggagggac aagctggtca acgccacgtc
     1441 ggcctacaac gtcctgcgca acctgggggg cagcgtgggc atcgcgctcg ccaccacgct
     1501 gctcgcccag cgcagccagt tccaccagca gaacctggtg agccacgtca cggtgtggga
     1561 ccccgcgacc caggagcggc tcgcccgctg gacgagccac ttcctggccc acggcagcga
     1621 cgcgttcacc gcgcagcgcc aggcgctggc catgctctac caggacgcgg tggggcaggc
     1681 gcaggtgctc gcctacgcgg acgacttctg gctcctcgcc atcctcttct cgatcgtccc
     1741 gctcttcctg cccctgatgc gccgggtgcg ggtggacgcg gtgtcgccga gcgaggaggc
     1801 ggagggcgag ggcggccgcg tgccgccgct gcccgcgccg gtcgactagt cggccgcggc
     1861 cgccgtcacc gggcggggac gggcgccctt cccggggtgg cgcgcctcga agcgggtgag
     1921 gacctcttcc cacgcgagtg accggcggcc gccccgccgg cgcagctccg gctgcagcac
     1981 aatcgggcgc tgccggatcc gacccgccag gttgctccgc accgcgcgct cggcgtagaa
     2041 gcgccggagg cgatggcaca tgagggagcg gcgcgcgttg tagccgaact cgcgacggta
     2101 ggcatccggc cccatgccgt gcgatcggag gtgggtgttg gtgagctggc ggagcgcgcg
     2161 gccgcagatc aggcagacga tcgtctcgtc ctggatcgcc cggcggggat cgtggtccaa
     2221 cagcgcgatt gggcgcggtg cgggctggat catgggggcc tccccggtat gaggtcgcgg
     2281 tcgccgccac ccctcgcaag tggcgagcca agccgggggt gtgatccgac ggggagttgc
     2341 ggcgcgcggg ccacccgcgc gagatgtccg acagcgcgtt cgacggtgtc gaagaccgtc
     2401 ggagcgtggt ttgacggatg cctgaccctt ggctactatc gctccactga tgggcggcct
     2461 ggcgggcctg atcggaaaca gccccctcat gctggcgctt cgagcgcaag tcgagcggct
     2521 ccttgcgcgc ccatcccccg cgggcggtcg tcttcctccc ctcctccttc tcggcgagac
     2581 cggtgttggt aaggggttgc tggtccgcct catgcatgag gcgagcgtgc gacgggagcg
     2641 ccccttgatc gagctgaact gcgccgcgat tcccgagagc ctggtcgagg ccgagctgtt
     2701 cggccacgag cggggcgcgt tcaccgacgc gcggcagagc cggccgggtc tcttccaggc
     2761 cgcgcacggg gggctgctgt tcctggacga ggtgaactcg ctgccgctcg cgacgcaggc
     2821 caagctgctc accgccatcg agcagcgcga ggtgcgccgg ctcggcagta cccgcgcgga
     2881 cccggtggac gcctggatcg tgagcgccac gaacacgcgg ctcgacgcgg cggtggcgcg
     2941 ccgggagttc cgcctcgatc tctaccaccg gatctccgcc gtcatcctcg agatgccgcc
     3001 gctccgcgcc cgcggccgcg acgtgctcgc gctcgccgag accttcctgg tgcgctcctg
     3061 cgccgagtac ggcctcgagc ccaagcgcct cggcaccgct gcggagggtg cgctcctcgc
     3121 ccacccgtgg cccggcaacg tccgggagct gcggaacgcg atcgagcggg cggtgctgct
     3181 cgccgacggc gacgagatca ccccggagct gatggagctg ccccggccgc ggagccatgc
     3241 ggccgcatcc ccgcggacgc gcccggaggg aggcgacgag cgggaatccc tccgcgacac
     3301 cctggaggcg gccggctgga acctctcgcg agccgcggcg cggctgggga tcccgcgcaa
     3361 caccctgcgc taccgcatcg agcgcctcgg actccgcccc ccggcggtcg acgtcgccgc
     3421 gccggagccg gcggggccgc cccccgcgcc ggcgaccgag ctgcgctggg agaagcgctg
     3481 gatcgccgcg gtgcaggtgc gcctggccct gccgcccgcg cggagcagct accacctgac
     3541 gccgctcctc gaggagctca tcgagaaggc ccggagcttc gggggacgcc tcgacgcgct
     3601 gcacccgctc gggttcaccg cgctctacgg catcgagccc atggaggccg ccccgacccg
     3661 cgccgcgctc gcggcccagg ccatcgtgaa cgcggcggcc ggctaccgcg aggcggacgg
     3721 cgagcgtgcc ggggccggct gccgggtgca cgccgcggag tgcctcatcg cgcgcggcgc
     3781 gccgatcgtc ggcatgagcg tcgaggatcg gcgccgcctg gacgaggccc tcgatgcgat
     3841 cgggatgggg cccggcctga tcgcggtgag cgcggaggcg gtcccgttcc tcgagcgtcg
     3901 cttcatcgtc gagcccgagg tggccgggac cgtgacgacc taccggctcg ccggtcccgg
     3961 ccgctcgggc ttcgacgtgg gcgggcagca gccgagcccg ttcgtcggcc gcgcccggga
     4021 gctcgcgcgt ctgcgcgacc tgctcgcggc cgcggagcgc ggccgcggcc aggtggtgga
     4081 cgtgatgggc gagcccggcg tgggcaagtc gcgcctgctg cacgagttcc ggcggacggt
     4141 ggggccggac cgcgtgctct atctcgaggg ccggtgcacc gcccacgggg cgagcacgcc
     4201 ctacctgccg ctcgtccatc tgctccagca gtgcttcggc ttcaccgagg ccgacgggtc
     4261 cgaggcgatc agggtccgcg ttcgcgaggg ggtggcgcgg ctcggtctct cggccgggac
     4321 ggtggcgccg tacctgctcc acctgctcgg ggtccccggg cgtccgggcg acggcgtcgc
     4381 gggctcgagc cccgagtcgg tgcgcgcgcg gaccgtcgac gcgctccgct acgtcatgat
     4441 cgcggccagc cagcagcgtc cggtgatcct cgcgatcgag gacctgcagt ggatggatcc
     4501 cacgtccgtc gcgagcctgg ccgtgctggc gccgagcgtg gccgcctcgc gcatcctggt
     4561 gctgacgacg tgccggcgcg gtcaccggcc gccctggctg ggccgcgcgc acgcgagcca
     4621 gatcgacctc gaccggctcg gccccgccga gagcctcacc atcgtccagg cgttgctcgc
     4681 gggccgcgag gtggcggccg gagtcgccga cgcgctggtc agcaaggccg acggcatccc
     4741 gttctttctc gaggagctcg cgcgcgtcat cgtggccgat ccggcctccc gggtcccggg
     4801 gatcccggat acgatccacg aggccatcgc ggcccgggtg gatcgactgg ccccggccga
     4861 tcgcgacctg ctgcagggtg ccgcggtgct cggccgggac gtgccccgcg cgctgctgcg
     4921 cgagatgtcg ggcctcgccg atccggagtt cggcatcgcg ctctcgcgcc tgcgcgtcac
     4981 cggcttcctg cgggagaccg cggccgatcc cgagacgtgc gggttcaccc acgtcctcac
     5041 ccaggagacg gcgtaccgga gcctcaccgc cgagcggcgg cgcgccctgc acgaggccgc
     5101 ggccgccgcc atcgagaagc gccagcccga gatcgccgac cgccatcccg aggtcctcgc
     5161 gcatcacctg accgaggcgg gcgccaccac cgcggcggcg tactggcacc gcgccgggcg
     5221 cctcgcgatc cgacgctccg ccaacgcgga ggccctcgcc cacctgcgcc gggccctggt
     5281 cctgctggaa gcgcagccgc ccggggccga gcgcgcgcgg gcggagctgg gtctccagct
     5341 gaccgtgggc gcggcgctgg tcgccgaccg cggctacggc gcgcgcgagc tcgacctgac
     5401 catgggccgc ctcgaggcgc tgatcggcga ggtcgtggac gcaccggagg tgccggtggc
     5461 gcgcttcgcc ctctgccggt tctccatcgc ccgggccgac ctcgtggcgg ccgggcgctt
     5521 cggtcaggag atgctgaccg cggcgggaag cgatcccgag gccgtgatcc cggcctcggt
     5581 cgcggccggc atcaccgact tctatctcgg gcgcttcctc cgggcgcggg agcacttcga
     5641 gcgcgtgctc gccgtgcacg agccccgctt cgcggaggcg cagatcgcgc gctacggcaa
     5701 cgacatgcgg gtggcggccc tgggcttcct cggctggacg tgcgccctca ccggggagct
     5761 cgatcgggcg gcccggctgg ccgaggagtg cgtcacccgg gcgcgggaga gcaggcatcc
     5821 gctcacgctg gcggtggcgc tcaactgggc gggcctggtc cggtgcgagc ggcacgagcc
     5881 cgcgcaggcg gcgcggctcg gcgaggagct gctcgatctc tcgcgcgagc agagcttcgc
     5941 cttcttcgcc gcgctcgccc tcggactgct cggctgggcc cgcctcgccc gcggtgagcg
     6001 cgcggcgggc gccgagctgc tgcggcaggg gttcgaccgc tatcgcgcgg ccaaccagcg
     6061 cgtcggcctg cggccccgcg cccaccacgt cgagggcctg ctgagcctgg gggcggtcga
     6121 cgaggcgctc gagcggctcg acgagttcat cgcgcattcc cgtgataccg gcgagcgcgg
     6181 gctcgtctcc gagctgttcc ggctcaaggg cgaggcgctc gcgcgtcgcg cccccgcgga
     6241 cccggaggcc acccgcgcgc tcgagcaggc ggtcgcgatc gcccaggagc agggagccac
     6301 cctcctcgcg ctgcgcgcgg cgatcggcct cgtccaggtc gagcgcgagc gcggcgacgc
     6361 gacgccgtcg ctcgccgcgc tgcgccgcgc ctaccagggc atgaccgagg gctccgacca
     6421 cccccacatg caggcggccc gagcgctcct cggggagtga ggccgtcgga ggaagcgccc
     6481 atgccgaccc tgctcacgaa ctggaatcgc tccgccgaca gcaagcccga ggtggtcgtc
     6541 cacccgaagt cggtggccga gctgcaggag atcgtg
//
LOCUS       DATMCV010000522         3849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_825217, whole genome shotgun sequence.
ACCESSION   DATMCV010000522 DATMCV010000000
VERSION     DATMCV010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825217"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VNU03_12045"
     CDS             <1..1212
                     /locus_tag="VNU03_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75241.1"
                     /translation="ARWASLAGPLAGAEVVDDLTVDVMTREPYGPILRTLAMYCTGIV
                     SPTAVQKMGEGFSRAPVGTGPFRFVEWKTNTHVILERNPDYWGDKALLDRVIFKVVPE
                     EGARMIALQTGDADMVLLPAPAQLAQIKRDPKYVVHEVVGGRVVFAGMNAAVPPLDDV
                     RVRTALLHAVDRKAILENILEGSAVAARGVLAPSVFGFKDMQLDRLYPFDRAKAKALL
                     AQAGWSPGPDGVLQKGGQRLSLSWLAARGRYPKDGEITEAIQAMFKEVGVEAKVQFLE
                     WATVFTQFRSAGFKDHMFTLGWVTSNADADYSMYALFHSKQVPPTGWNTSRYANPKVD
                     ALVEQARRNLNQTEREKQYGEAQDILAREMVWIPVYNTKEIVVTRAGVKGFTVHPVEY
                     NLWLGKTWLDK"
     gene            1229..2152
                     /locus_tag="VNU03_12050"
     CDS             1229..2152
                     /locus_tag="VNU03_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75242.1"
                     /translation="MLTYVARRLLLAVPVLLGVVFVVMLTVELIPGDAVALMLGEHAT
                     PEAVARLRDYLGLDKPLMVRYAAYVGHVARGDLGRSIQQNRPVVDELADAWPATLELT
                     VAALVLAAAAGVAAGVVSAVWPNSLFDAVARIGSLFGLSMPVFWTGLVLIVIFALWLP
                     WLPVGGTGSLGHLVLPAVTLALPSVAMVARMTRSSVLEVLREDYVRTARAKGVAERLV
                     VSKHALRNAAIPILTLLGLQAGQLMGGAVLTETVFAWPGLGRLMVKAIFARDYVLLQG
                     AVLVFALAFVLINLVVDLAYGALDPRIARQG"
     gene            2156..3052
                     /locus_tag="VNU03_12055"
     CDS             2156..3052
                     /locus_tag="VNU03_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75243.1"
                     /translation="MAVGARVLDEPAGLAAPATLSADAWQRFRRSRLAMVGLVVVALL
                     VLAAAAAPWLAPADPAKQSLIEKRARPGGKFILGADEFGRDILSRLIFGTRVALLVGG
                     LSVAIALAVALTLGCLAGFAGGWIDVVVMRIVEILLAFPYLLLAIAVVSALGPGVLNT
                     TIAVGIWGAPTATRLVRAAVLLARDSEYARAARALGASAGRVLTRHVLRNVLPTVIVY
                     STLFMANAILVEAALSFLGLGAQPPTPSWGLMVSSGRDFLLVAPHIATVPGLAIMVAV
                     LGFNLLGDGLRDALDPRLRGQL"
     gene            3055..3831
                     /locus_tag="VNU03_12060"
     CDS             3055..3831
                     /locus_tag="VNU03_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018115997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75244.1"
                     /translation="MRLEERVAIVTGAAHGIGRAICVELAREGARVWACDVRAPELAA
                     TRAAVDAVRPGAGQTAAVDVRDARAVADFVARVDGAAGRVDILVNTAGGVSGQVMRPV
                     EEVSDEAWREIFAVNLDGAFHFTRAVAPAMKRAGRGAIVNISSGAGRSYSLTGIQAYA
                     SAKAGLIGFTRQTARELGRHGIRVNSVAPGFMRTNPATEVQWEAMGATGQARLMDSIA
                     LGRLGAPEDVARAVVFFVSDDAGYVTGQTISVDGGQWMLG"
BASE COUNT          467 a         1365 c         1430 g          587 t
ORIGIN      
        1 gcacggtggg cgagcctcgc cggcccgctc gcgggcgcgg aggtggtgga cgacctcacc
       61 gtcgacgtga tgacgcgcga gccctacggc cccatcctcc gtaccctcgc gatgtactgc
      121 accggcatcg tctctccgac cgcggtgcag aagatggggg aggggttcag ccgcgccccg
      181 gtcggcaccg ggcccttccg attcgtggag tggaagacca acacccacgt catcctggag
      241 cgcaaccctg actactgggg cgacaaggcg ctgctcgatc gggtgatctt caaggtggtg
      301 ccggaggagg gggcgcggat gatcgcgctc cagaccggcg acgcggacat ggtgctgctg
      361 cccgcgccgg cccagctcgc tcagatcaag cgcgatccca aatatgtcgt ccacgaggtg
      421 gtgggcgggc gcgtggtctt cgcgggcatg aacgcggcgg tcccgccgct cgacgacgtg
      481 cgggtgcgca ccgccctgct ccacgcggtg gaccgcaagg cgatcctcga gaacatcctg
      541 gagggctcgg cggtggccgc gcgcggggtg ctggccccga gcgtgttcgg cttcaaggac
      601 atgcagctcg accgcctcta tcccttcgac cgcgcgaagg ccaaggccct gctggcccag
      661 gccggctgga gcccgggccc cgacggcgtc ctccagaagg gcgggcagcg actctcgctc
      721 tcctggctgg ccgcgcgcgg gcgctatccg aaggacggcg agatcaccga ggcgatccag
      781 gccatgttca aggaggtggg ggtcgaggcg aaggtacagt tcctcgaatg ggccaccgtg
      841 ttcacccagt tccgctccgc cggcttcaag gaccacatgt tcacgctcgg ctgggtgacc
      901 tcgaacgcgg acgccgacta ctcgatgtac gcgctcttcc acagcaagca ggtcccgccc
      961 accggctgga acacctcgcg ctacgcgaac ccgaaggtcg acgcgctggt cgagcaggcg
     1021 cgccgcaacc tgaaccagac cgagcgcgag aagcagtatg gcgaggcgca ggacatcctg
     1081 gcccgcgaga tggtgtggat cccggtctac aacaccaagg agatcgtggt gacccgggcc
     1141 ggggtcaagg gcttcacggt ccatcccgtg gagtacaacc tctggctcgg taagacctgg
     1201 ctcgacaagt agggccggag cggccgtcgt gctgacctac gtggcgcgcc ggctcctcct
     1261 cgcggtgccg gtgctgctgg gcgtggtctt cgtggtcatg ctcaccgtcg agctgatccc
     1321 gggcgacgcg gtcgccctca tgctcggcga gcacgccacc ccggaggcgg tggcccggct
     1381 gcgcgactac ctgggcctcg acaagcccct gatggtccgc tacgcggcgt acgtggggca
     1441 cgtcgcgcgc ggcgacctcg gccgctccat ccagcagaac cgcccggtgg tggacgagct
     1501 ggccgacgcg tggccggcca ccctcgagct gacggtggcc gcgctggtgc tggccgccgc
     1561 ggccggggtc gccgccgggg tggtctccgc ggtctggccc aactcgctct tcgacgcggt
     1621 ggcccggatc ggctcgctct tcggcctgtc catgccggtg ttctggaccg gtctggtgct
     1681 gatcgtgatc ttcgcgctct ggctgccgtg gctgccggtg ggcggcaccg gctcgctcgg
     1741 ccacctagtg ctgcccgcgg tgaccctcgc cctgccgtca gtggcgatgg tggcgcgcat
     1801 gacgcgctcg agcgtgctcg aggtgctgcg cgaggactac gtgcgtaccg cgcgggccaa
     1861 gggggtggcc gagcggctgg tcgtctccaa gcacgcgctg cgcaacgcgg cgatcccgat
     1921 cctcaccctg ctcggcctgc aggccggcca gctcatgggc ggcgcggtgc tgaccgagac
     1981 cgtcttcgcg tggcccgggc tcgggcgcct catggtcaag gcgatcttcg cccgcgacta
     2041 cgtgctgctg cagggcgcag tgctcgtgtt cgcgctcgcc ttcgtgctga tcaatctggt
     2101 ggtggatctg gcctacggcg cgctcgatcc acgcatcgcg cggcagggct aggcgatggc
     2161 ggtcggcgcg cgcgtgctcg acgaacccgc cgggctcgcc gcgcccgcga ctctgtccgc
     2221 ggacgcgtgg cagcgcttcc ggcggagccg gctcgcgatg gtggggctgg tggtggtcgc
     2281 gctgctggtg ctcgcggccg cggcggcgcc gtggctggcc cccgccgatc cggccaagca
     2341 gagcctgatc gagaagcggg cccggccggg cggcaagttc atcctgggcg ccgacgagtt
     2401 cggccgcgac atcctctccc gcctgatctt cggcacccgg gtggcgctcc tggtgggcgg
     2461 gctctcggtg gcgatcgcgc tcgcggtggc gctgaccctg ggctgcctgg ccggcttcgc
     2521 gggcggctgg atcgacgtcg tcgtcatgcg aatcgtcgag atcctgctcg ccttccccta
     2581 cctgctcctg gccatcgcgg tggtgtcggc gctcggcccg ggcgtgctca acaccacgat
     2641 cgcggtcggc atctggggcg cccccaccgc gacgcgcctg gtgcgcgcgg cggtgctgct
     2701 cgcgcgcgac tccgagtacg cccgggccgc ccgcgcgctc ggggcctcgg ccggccgcgt
     2761 gctcacccgc cacgtgttgc gcaacgtgct gccgaccgtg atcgtctact ccacgctgtt
     2821 catggccaac gccatcctgg tcgaggccgc gctgtcgttc ctgggcctcg gggcccagcc
     2881 gccgacgccc tcgtgggggc tcatggtctc gagcgggcgc gacttcctgc tggtggcgcc
     2941 gcacatcgcg accgtccccg gcctcgccat catggtcgcg gtgctcggct tcaacctgct
     3001 cggcgatggg ctccgcgacg ccctcgaccc gcggctgcgg gggcagctct agccgtgcgg
     3061 ctcgaggagc gggtggccat cgtgaccggc gccgcgcacg gcatcggccg cgcgatctgc
     3121 gtggagctgg cccgcgaggg cgcccgggtg tgggcctgcg acgtgcgcgc gcccgagctc
     3181 gccgcgaccc gcgccgcggt ggacgcggtc cggcccgggg ccgggcagac cgcggcggtc
     3241 gacgtgcgcg acgcgcgcgc ggtggccgac ttcgtggcgc gggtggacgg ggcggccggg
     3301 cgcgtcgaca tcctggtcaa caccgcgggc ggcgtgtcgg ggcaggtgat gcgcccggtc
     3361 gaggaggtgt cggacgaggc gtggcgcgag atcttcgcgg tcaacctcga cggcgcgttc
     3421 cacttcacgc gcgcggtggc gcccgcgatg aagcgcgccg gccgcggcgc gatcgtgaac
     3481 atctcatccg gtgccggccg gtcctacagc ctcaccggca tccaggccta cgcgagcgcc
     3541 aaggcgggcc tgatcggctt cacccggcag accgcgcggg agctggggcg tcacggcatc
     3601 cgggtcaact cggtggcgcc cggcttcatg cgcaccaacc cggccaccga ggtccagtgg
     3661 gaggcgatgg gcgcgaccgg gcaggcgcgg ctgatggact cgatcgccct cggacgcctc
     3721 ggcgcgccgg aggacgtggc gcgcgcggtg gtcttcttcg tgtcggacga cgcgggctac
     3781 gtcaccggcc agaccatcag cgtcgacggc gggcagtgga tgctcggctg aggagtcggc
     3841 ccgcgaccg
//
LOCUS       DATMCV010000523         3817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_691389, whole genome shotgun sequence.
ACCESSION   DATMCV010000523 DATMCV010000000
VERSION     DATMCV010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691389"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VNU03_12065"
     CDS             complement(<1..814)
                     /locus_tag="VNU03_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP75245.1"
                     /translation="MTRIDPVWAGVTELSQAFAARTLSPVDTVDALLERIERRDPLLH
                     SFIAVYADDARLAAEGADRAIRSGHRMGPLHGVPIALKDLVDLEGRVTTGGSKVWANR
                     VSPVTATLAERAIAAGMIVLGKTHTVEFAMGSWGTNTHMGTPRNPWDLAVHRTPGGSS
                     SGSAVAVAAGLAPIAIGTDTGGSVRLPAGWCGIVGLKVTAGRISTYGVLPLSFTLDTP
                     GPIARSVEDAALLFRLLNGPDPRDPQTLSWTAADPLPTLRRGVAGLRIAVMPD"
     gene            complement(811..2031)
                     /locus_tag="VNU03_12070"
     CDS             complement(811..2031)
                     /locus_tag="VNU03_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75246.1"
                     /translation="MRRVLTNATVIDCVTPRPIPGASVVIERGRIVEVLDGSRSPDTR
                     SADVVDLGGAYLLPGLWDVHIHPDYLASTGASVAEQTAIFGHRLMEALTESGVVGVRC
                     AGAAHFMDVAWKRAFDAGQYVGPRVFAAGHFLTTTGGHFLTSGHARECDGPYGFVRAI
                     REQIKNGVDHIKLNLTGGIMGPAWDRHTQSFLLDEELDAAFAICRKRGFPVMAHAANP
                     ETVAAAARLGAHSVEHGYIMDDACIEALRAHATWYVPTLAITQLTPDQARDPWEKRWV
                     EQRELAPDLCRRADDAAEPHRDAFRRALAAGVPMALGSDIRPLKDAALLELGLWVAAG
                     ATPWQALLAATRHAATLCGVGDDLGTIEPGKLADLIVVAANPLEHITNLRRLLLVLKE
                     GRIVSDKRATEPST"
     gene            complement(2053..2436)
                     /locus_tag="VNU03_12075"
     CDS             complement(2053..2436)
                     /locus_tag="VNU03_12075"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWP75247.1"
                     /translation="MGPTIDLLLQINAAFNSRDVNRIMAFFADDATFLMARGPEPCGR
                     RVSGKAAIGQVLADRFKVIRDMRWDHIDHFVTGDRAVSIWTVTGTGADGERLDYQGCD
                     IYEFRGDKILNKDTYWKVVEQRPRL"
     gene            complement(2439..3335)
                     /locus_tag="VNU03_12080"
     CDS             complement(2439..3335)
                     /locus_tag="VNU03_12080"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP75248.1"
                     /translation="MPGLHEVNVIDTPYARPAGLELLARVYRPRDAAPTPRAALVYVH
                     GGAWARLDRTADAILCEALAASGRIVVALDFRQAPDHRFPAASADVAAGVRYTRAHAG
                     RLGVDPARIGLLGSSSGGHLALLHAVHPGAPEGAGTPVVTPDGALDASPRDAAVAFVV
                     ALYPVADPLARYRYALSREHEPPPPSGFDAKRLIASHRAFFADEAAMAGASITRLVAE
                     GLATARPPVWVAQPDLDDNVPAAITQAFVDAYRKAGGHLERVHFPESRHGFAQQASPA
                     TDKCVADVLDFVGRQTGPAQGA"
     gene            complement(3353..>3817)
                     /locus_tag="VNU03_12085"
     CDS             complement(3353..>3817)
                     /locus_tag="VNU03_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWP75249.1"
                     /translation="QALPIDPATGEKISLIDVTAQPRYESGGGGGVSTTDDYVRFAQM
                     LLDGGHLGATRLLSRTSVAFMASDHLGPISGTMRRPGYTFGLGFQVRKDVGLDGQSGS
                     IGEYGWAGAGGTYFWIDPKEQLIAILMTQAPGPSRTYYRQLFKELVQQALVD"
BASE COUNT          509 a         1313 c         1472 g          523 t
ORIGIN      
        1 cgtcgggcat caccgcgatc cgcaggccgg ccacgccgcg ccggagggtg ggcaggggat
       61 cggccgcggt ccacgagagc gtctgcggat cccgcgggtc gggcccgttg agcaggcgga
      121 agaggagcgc ggcgtcctcc acggaccgcg cgatcggccc cggcgtgtcg agcgtgaagg
      181 agagcgggag gaccccgtag gtgctgatgc gccctgcggt caccttcagg ccgacgatcc
      241 cgcaccagcc ggcgggcagc cgcaccgagc cgccggtgtc ggtgccgatc gcgatcgggg
      301 cgaggccggc cgcgaccgcg accgcggaac cgctggagga gccgcccggc gtgcggtgca
      361 ccgcgagatc ccacggattc cgcggggtgc ccatgtgggt gttggtgccc cagctcccca
      421 tcgcgaactc gacggtgtgg gtcttgccga gcacgatcat gcccgccgcg atcgcgcgct
      481 cggccagggt ggcggtgacc ggcgagacgc ggttggccca gaccttcgag ccgccggtcg
      541 tgacccggcc ctcgaggtcg accaggtcct tgagcgcgat gggcacgccg tggagcgggc
      601 ccatccggtg gccggagcgg atcgcgcggt cggcgccctc ggcggcgagg cgggcatcgt
      661 ccgcgtagac cgcgatgaag gagtgcagca gcgggtcgcg acgctcgatg cgctcgagca
      721 gggcgtcgac cgtgtcgacg ggagagaggg tccgggccgc gaaggcctgg gacagctcgg
      781 tgacgccggc ccacacggga tcgatcctgg tcatgtcgat ggctccgttg cgcgcttgtc
      841 cgagacgatg cgcccctctt tgagcacgag gagcagtcgg cggaggttgg tgatgtgctc
      901 gagggggttc gcggccacca cgatcaggtc cgcgagcttg ccgggctcga tggtgccgag
      961 gtcgtcgccc acgccgcaga gcgtggccgc gtgccgggtg gccgccagca gcgcctgcca
     1021 cggggtcgcg cccgcggcca cccagaggcc gagctcgagc agagccgcgt ccttgagcgg
     1081 gcggatgtcg gagccgagcg ccatcgggac gcccgcggcg agcgcgcggc gaaaggcatc
     1141 gcggtggggc tcggccgcgt cgtcggcgcg ccggcagaga tccggggcga gctcgcgctg
     1201 ctccacccat cgcttctccc acggatcgcg ggcctggtcc ggcgtgagct gcgtgatcgc
     1261 gagcgtcggg acgtaccagg tggcgtgcgc acgcagcgcc tcgatgcagg cgtcgtccat
     1321 gatgtagccg tgctcgacgc tgtgggcgcc gaggcgggcc gccgcggcca ccgtctccgg
     1381 gttcgcggcg tgcgccatga ccgggaagcc gcgcttgcgg cagatcgcga aggcggcgtc
     1441 cagctcctcg tcgaggagga acgactgggt gtggcggtcc cacgcgggcc ccatgatgcc
     1501 gccggtcagg ttcagcttga tgtggtcgac cccgttcttg atctgctcgc ggatcgcgcg
     1561 cacgaagccg tagggcccgt cacactcccg cgcgtgaccg gaggtgagga agtgcccccc
     1621 ggtcgtggtc aggaagtggc cggccgcgaa gacccgtggg cccacgtact ggcccgcgtc
     1681 gaaggcgcgc ttccacgcca cgtccatgaa gtgggccgcg cccgcgcacc gcaccccgac
     1741 caccccgctc tcggtcaggg cctccatgag gcgatggccg aagatggcgg tctgctcggc
     1801 caccgaggcc ccggtggagg ccaggtagtc gggatggatg tggacgtccc acaggcccgg
     1861 cagcaggtag gcgccgccga gatcgacgac gtcggcgctc cgcgtgtccg gcgatcggct
     1921 cccgtcgagc acctccacga tgcggccgcg ctcgatgacc acgctggcgc cgggaatggg
     1981 ccgaggggtg acgcagtcga tcaccgtggc attggtcagg acgcgtcgca tcgtcacggt
     2041 cgctcccgcc cgtcagaggc gggggcgctg ctccaccacc ttccagtagg tgtccttgtt
     2101 caggatcttg tcgccgcgga actcgtagat gtcgcagccc tggtagtcga gccgctcgcc
     2161 gtcggcgccg gtgccggtca ccgtccagat cgagaccgcc cggtcgccgg tcacgaagtg
     2221 gtcgatgtgg tcccagcgca tgtcgcggat gaccttgaag cggtccgcca gcacctggcc
     2281 gatggccgcc ttgccgctca cccggcgccc gcacggctcc ggaccgcggg ccatcaggaa
     2341 ggtggcgtcg tctgcgaaga acgccatgat ccggttcacg tcgcggctgt tgaaggccgc
     2401 gttgatctgg agcagcaggt cgatggtcgg tcccatggtc aggctccctg agcgggcccg
     2461 gtctgccggc cgacgaagtc cagcacgtcc gcgacgcact tgtcggtggc cgggctcgcc
     2521 tgctgggcga agccgtgacg ggactcggga aagtgcacgc gctcgaggtg gccgcccgcc
     2581 ttccggtagg cgtcgacgaa ggcctgggtg atcgcggcgg gcacgttgtc gtccaggtcg
     2641 ggctgcgcga cccagaccgg cggccgggcc gtggccaggc cctccgcgac gagccgggtg
     2701 atgctcgcgc ccgccatcgc ggcctcgtcc gcgaagaagg cgcggtgcga ggcgatcagc
     2761 cgcttggcgt cgaacccgga cggcgggggc ggctcgtgct cgcgggagag ggcgtagcgg
     2821 tagcgggcga gcggatcggc gaccgggtag agcgccacca caaaggccac cgccgcgtcg
     2881 cgtgggctcg cgtcgagggc gccgtccggt gtcaccaccg gcgtgcccgc tccctcgggc
     2941 gccccggggt gcaccgcgtg cagcagggcc aggtggccgc cgctcgagct gccgagcagg
     3001 ccgatacgcg cgggatcgac gccgaggcgc ccggcgtggg cacgggtgta gcggacgccg
     3061 gccgccacgt cggcgctcgc ggcggggaag cggtggtcgg gcgcctggcg gaagtcgagc
     3121 gcgaccacga tgcggccgga cgcggcgagc gcctcgcaca ggatcgcgtc ggcggtgcga
     3181 tccaagcgtg cccaggcccc gccgtggacg tagaccagcg ccgcgcgcgg cgtcggcgcc
     3241 gcgtcccgcg ggcggtagac gcgggccagc agctcgagcc cggccgggcg cgcgtacggc
     3301 gtgtcgatca cgttcacttc gtgaagtccg ggcatgggct tcgtctccgt gctcagtcga
     3361 ccagcgcctg ctgcaccagc tccttgaaga gctgccggta gtaggtccgc gaggggcccg
     3421 gggcctgggt catcaggatc gcgatgagct gctccttcgg gtcgatccag aagtaggtgc
     3481 cgcccgcgcc ggcccagccg tattcgccga tcgagcccga ctggccgtcc agccccacgt
     3541 ccttgcgcac ctggaagccg agcccgaacg tgtagccggg ccggcgcatg gtgccgctga
     3601 tcggcccgag gtgatcggac gccatgaagg cgacggaggt gcggctcagc agccgggtgg
     3661 ccccgaggtg gccgccgtcg agcagcatct gcgcgaagcg cacgtagtcg tcggtggtgg
     3721 acactccacc gccgcctccg gattcatagc gcggctgggc ggtcacgtcg atcagcgaga
     3781 tcttctcgcc ggtggccgga tcgatcggga gcgcctg
//
LOCUS       DATMCV010000524         2056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_628490, whole genome shotgun sequence.
ACCESSION   DATMCV010000524 DATMCV010000000
VERSION     DATMCV010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628490"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VNU03_12090"
     CDS             complement(<1..199)
                     /locus_tag="VNU03_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018302364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75250.1"
                     /translation="MAANAPALVRVRPERLAGVGGRRRGRLIVGAALVGLFAVSALLA
                     PWLAPYDPAAVPAALSDTMLRP"
     gene            complement(189..1130)
                     /locus_tag="VNU03_12095"
     CDS             complement(189..1130)
                     /locus_tag="VNU03_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75251.1"
                     /translation="MISDLVEPAAKRVLALLPVLLGVSLLVFALTAVGLGDPVRAMLG
                     QRADPELVEQIRRDYALDRPVYERYVAWMSRLLRGDLGTSYHQQRPVAEIIAERLPAT
                     ARLAVAATALSVVVGMGAGVMAALRPGRFLDQLLMSAAVLGISTPVFWLGMMLSLLFA
                     VWLGWLPVSGYGDGAWANLVLPALTLGAVHTGTIARMARSSLLEVVRQDYIQNARAKG
                     LGEWRVMAKHALRNALIPVVTVIGIGLADLLVGAPLTETVFAWPGIGRMLVAAVGQRD
                     LPVVMGAVLVFAVIYVMANMVVDLAYLVIDPRIRRGR"
     gene            complement(1285..>2056)
                     /locus_tag="VNU03_12100"
     CDS             complement(1285..>2056)
                     /locus_tag="VNU03_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75252.1"
                     /translation="QVAIWPTLGTHAVRFNVEKPPFNDRRIRLAVAHAIDPSGVVQGL
                     LEGCVIAGKGILPPALPGYNPEVTAPGFDRAKARALLAEAGFPAGRGLGPITFNFNTG
                     DLNQRIAELLQAQLREVGITLELRRLDWAAHLKVVDAGAMTMFRQGWIADYPDPENFL
                     TVLFHSRNVGAAGNTSRYRSPELDRLFDQADSMAAGPDRMKLYQGAEQRLLDDGAWVT
                     LYHYASRALIRPVVKGLERSPLSTAPEFLAPLRKVYLE"
BASE COUNT          322 a          722 c          754 g          258 t
ORIGIN      
        1 gcgggcgcag catggtgtcg ctgagcgcgg cggggaccgc ggccgggtcg tacggcgcga
       61 gccagggggc caggagggcg gacaccgcga agaggccgac gagcgccgcg ccgacgatga
      121 ggcggccccg ccggcggccg ccaacccccg cgagccgctc cggccgcacg cgcacgaggg
      181 cgggggcgtt agcggccacg ccggatccgt gggtcgatga ccaggtaggc gaggtccacg
      241 accatgttgg ccatcacgta gatgaccgcg aagacgagca ccgcccccat caccaccggc
      301 aggtcccgct ggccgaccgc ggccaccagc atccgtccga tgcccggcca ggcgaacacg
      361 gtctcggtga gcggcgcgcc caccagcagg tcggcgagcc cgatgccgat cacggtgacg
      421 acggggatca gggcgttgcg cagggcgtgc ttggccatca cccgccattc gcccaggccc
      481 ttggcgcgcg cgttctggat gtagtcctgg cggacgacct ccaggaggct cgagcgcgcc
      541 atgcgcgcga tggtcccggt atggaccgcc cccagcgtca gcgcgggcag caccaggttc
      601 gcccaggctc catcaccgta accggacacc gggagccacc cgagccagac cgcgaagagc
      661 agcgagagca tcatgccgag ccagaacacc ggggtggaga tccccagcac cgccgcactc
      721 atcaggagct ggtcgaggaa gcgcccgggg cggagcgcgg ccatcacacc cgcacccatg
      781 ccgacgacga ccgagagtgc ggtggcggcc accgccaggc gcgcggtggc gggcagccgc
      841 tccgcgatga tctcggccac cgggcgctgc tggtgatagg aggtccccag atcgccccgc
      901 agcaggcgcg acatccaggc cacgtagcgc tcgtacaccg ggcgatcgag cgcgtagtcg
      961 cgccggatct gctcgaccag ctcgggatcg gcgcgctgcc ccagcatggc gcgcaccgga
     1021 tcgccgagcc ccaccgcggt gagcgcgaac accagcaggc tgacgccgag caggaccggc
     1081 agcagggcca gcacgcgctt ggcggcgggc tcgactaggt cgctgatcat gtgggagagg
     1141 gcggtcagcc ggcgcgctgg cgcgcgcttg cttgtgctgc cgtttttttc ttcatctgcg
     1201 ggggcccaga aatggccccc gcactccccc aacggcgggc atccggctgc gagcccgccc
     1261 gcggacaacc gttggcgtca ccttctactc caggtagact ttgcgcaggg gcgcgaggaa
     1321 ctcgggggcg gtggacagcg gggagcgctc gagccccttg acgacggggc ggatcaaggc
     1381 gcggctcgcg tagtggtaga gcgtcaccca cgccccgtcg tcgagcagtc gctgctcggc
     1441 gccctggtac agcttcatgc ggtcgggccc cgccgccatc gaatcggcct gatcgaacag
     1501 gcggtcgagc tccgggctgc gatagcggga ggtattgccg gccgcaccca cgttgcgcga
     1561 gtggaagagc acggtgagga agttctcggg gtccgggtag tcggcgatcc agccctggcg
     1621 gaacatggtc atcgcgcccg cgtccaccac cttcagatgg gccgcccagt cgaggcgccg
     1681 cagctcgagg gtgatcccga cctcgcggag ctgggcctgc aggagctcgg cgatccgctg
     1741 gttgagatcg ccggtgttga agttgaacgt gatcggtccg agcccgcggc cggccgggaa
     1801 gcccgcctcc gccagcaggg cgcgggcctt ggcgcggtcg aagccgggtg cggtcacctc
     1861 cgggttgtag ccggggagcg ccggtggcag gattcccttc cccgcgatga cgcagccctc
     1921 gagcagcccc tgaaccacac cgctcggatc gatggcgtgg gccaccgcca gccggatgcg
     1981 ccgatcgttg aacgggggct tctccacgtt gaagcgcacc gcgtgggtcc cgagagtcgg
     2041 ccagatcgcg acctgg
//
LOCUS       DATMCV010000525         6841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_560448, whole genome shotgun sequence.
ACCESSION   DATMCV010000525 DATMCV010000000
VERSION     DATMCV010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560448"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VNU03_12105"
     CDS             complement(<1..168)
                     /locus_tag="VNU03_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75253.1"
                     /translation="MDILLQQVVSGLATGGIYASLALALVMIYQATDVVNFAQGNMAM
                     FSTYIAWSLLQA"
     gene            complement(299..1012)
                     /locus_tag="VNU03_12110"
     CDS             complement(299..1012)
                     /locus_tag="VNU03_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75254.1"
                     /translation="MPALLEAEDLAAQYGWTKVLHGLSFAVESGGITTILGANGAGKT
                     TTLRAVCRMVKTSGQVRFDGQRIDGKATEDIVRLGIAHTPEGRGTFVDLSVEENLRLG
                     AYARREKSGLGQDYEKVYGYFPVLAQRRRQQAGTLSGGEQQMLAVARALMSRPRLLLL
                     DEPSLGLAPLVVREIFRIVRTINQQERVSVLLVEQNAAMALDLADHAYLLETGRVVMS
                     GPAAELKKNDAIRRSYLGY"
     gene            complement(1012..1746)
                     /locus_tag="VNU03_12115"
     CDS             complement(1012..1746)
                     /locus_tag="VNU03_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75255.1"
                     /translation="MRFGGIVALDDVSFDMAPGHIVGLIGPNGAGKTTLFNCVSRLYT
                     PSSGDLLFEGKSIIKNTPHHVAALGIGRTFQNLALFPSMTVLQNVMVGVHARTGSDFL
                     SNALRLPSVKREERMMREAALEAIAFLQLDAVIGHPAAGLPFGTLKRVELARAIAGNP
                     KLLLVDEPAGGLNHEEVAALGDILRSIRDRRGVTVLLVEHHLSLVMQVSDRVVVLDFG
                     RRIAEGPPAAIQRDPDVIRAYLGSES"
     gene            1869..3548
                     /gene="ettA"
                     /locus_tag="VNU03_12120"
     CDS             1869..3548
                     /gene="ettA"
                     /locus_tag="VNU03_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HWP75256.1"
                     /translation="MAHQYIYVMKDLKKIVPPKREILKGIWLSFYPGAKIGVIGGNGA
                     GKSTLLRIMAGVEKEFLGEAWPADGVRVGYLPQEPQLDPAKDVRGNVEDGVGEVRSLL
                     TRFDEINARLGEPLGDDEMEKVLAEQSRVQDAIEAANGWDLDRTVEIAMDALRCPPGD
                     AEVSTLSGGEVRRVALCRLLLQKPDLLLLDEPTNHLDAESVAWLERFLKDYPGTVVAI
                     THDRYFLDNVAGWILELDRGAGIPWEGNYSSWLDQKKQRLAIEEKQETARQRTLEREL
                     EWVRMSPRARQAKSKSRLQAYEELLAEGGRDREGQAEIIIPTPSRLGDLVVQTEHLVK
                     GYGDKLLIEDLTFSLPRGGIVGVIGPNGAGKTTLFRMITGQEKPDRGALQVGETVRLA
                     YVDQSRDALDPAKNVWQEISGGAEQLQLGTRQIASRAYVASFNFKGSDQQKRVGDLSG
                     GERNRVHLATMLKSGGNLLLLDEPTNDLDVDTLRALEDALLSFAGCAVVISHDRWFLN
                     RVATHMLAFEGDSRVVWFEGNYEDYEADRHKRLGAAADTPHRIRYKPLTRG"
     gene            3580..4212
                     /locus_tag="VNU03_12125"
     CDS             3580..4212
                     /locus_tag="VNU03_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWP75257.1"
                     /translation="MFKDIVASREELRALFGAPSERAVQKDRGRLDAHTRAFIARSPF
                     VLIATAGAAGRCDVSPKGDAPGFVMVLDDTHLAIPDRVGNNRIDGLTNIVENPQLGLI
                     FLIPGREDTLRVNGRACIVRDEEILSRLEVQGKRPKVAIGVEIEECFLHCAKAFKRSG
                     LWDRERWPDVASLPSMAKILWEQIDLQATAQCSVDDFERDMEDRLRKGLY"
     gene            4241..5371
                     /locus_tag="VNU03_12130"
     CDS             4241..5371
                     /locus_tag="VNU03_12130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP75258.1"
                     /translation="MSPGLPRAADIVVIGAGAIGASIAYHLGRRGARDVVVLERDAVG
                     AGSTSKAAGGIRVQFATRVEIEFSQRGIAFFQRFEDEMGVPCDFHQEGYLFVVTDEPT
                     LTRFRANVALQRSLGADVRIISPDDARALVPSLAMDDALAAVWGPTDGHASPNDVVQA
                     YAARARALGVRILEDTPVTGIRLEGGRVAAVETPAGPIATRLVVNAAGPWAPLVGRMA
                     GLDLPVDPRRRHIFVTDAFDGIRHPMPLVTDTASGFYCRSEQGAVLMSPGDVGASTAY
                     EAQVDWSVLEAAVEKAVRRVPALERAQVRHAWAGLRPLTPDGRAILDWAPGVDGLYLA
                     VGFCGHGFQHSPAVGETVAELLLEGRTAVDLSDLRLGRFAVA"
     gene            5461..>6841
                     /locus_tag="VNU03_12135"
     CDS             5461..>6841
                     /locus_tag="VNU03_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP75259.1"
                     /translation="MPDVSLARLRQVASLARSVAGTLDADAVLREVVEALRTLRPGAF
                     CVVRLVDAERGGYRLAAMSGSDPHAVAAVIPFGHGLTHAVAQSGHPMLLSDVSGDPRL
                     VSAKWREDSGLKAYYGVPITAGGELMGVLSVALADHDEPTAEEREIVDLLASHAAVAI
                     GNARLFAESETRRRAAEALSEVWRFLSETFDVAALGQRIADVVLRALQVRAAALYRLV
                     PASGDLEVIGRSGEAERFLDPGFVIPAGYGMVGLAVNRRQPVVTPDIRADDRVKRTPG
                     MQVHHAICPSVLSLPLVVKGQVVGGFTVGDTLGRAYSDDDLRLAQTFADQAAVALENA
                     RLFEEAERRRWQSLALGAVSQTLAESLHPSGVAERVVDAARELLGVRVATLYHLDPIT
                     GALEESACAGRLPGRAWSLRHPRGTGAVGLAAAERRAVVSNDVLLDPRLTFAPEMRAH
                     IEGTGLRAVM"
BASE COUNT         1008 a         2376 c         2452 g         1005 t
ORIGIN      
        1 cgcctgcagc agggaccagg cgatgtaggt actgaacatc gccatgttgc cctgggcgaa
       61 gttcaccacg tcggtggcct ggtagatcat cacgagggcc agggccaggc tcgcgtagat
      121 gcctccggtg gcgagcccgg agactacctg ctggagcagg atgtccattt taagtggggg
      181 ggagcgagcg ccgctcctcc cccacgcccc cccaccgctt cgaagcttgc gccgagactc
      241 gcgctggttg gttcatcggt cggtgttcgg tgcgctcatc aattggttca tcgaaccctc
      301 agtatccgag ataggagcgg cggatggcgt cgttcttttt tagctcggcg gcggggcccg
      361 acatcacgac gcggccggtc tcgagcagat aggcgtggtc ggctaagtcg agggccatcg
      421 ccgcgttctg ctccaccagg aggacgctca cgcgctcctg ctgattgatc gttcgcacga
      481 tgcggaagat ctcgcggacc accaggggag cgaggccgag cgagggctcg tcgagcagga
      541 gcaggcgggg gcgcgacatg agggcccgcg cgacggccag catctgctgc tcacctcccg
      601 agagcgtgcc cgcctgctgc cggcgccgct gggcgaggac cgggaagtac ccgtagacct
      661 tctcgtagtc ctggccgagg cccgacttct cccgcctcgc gtaggctccg aggcggagat
      721 tctcctcgac gctcaggtcc acgaacgtgc cgcgaccttc cggggtgtgg gcgatgccga
      781 ggcgcacgat gtcctcggtg gccttgccgt cgatccgctg gccgtcgaag cgcacctggc
      841 cgctggtctt caccatgcga cacaccgccc ggagcgtcgt ggtcttgccg gctccgttgg
      901 cgccgaggat cgtggtgatg ccgccgctct ccaccgcgaa cgagagcccg tggagcacct
      961 tggtccaccc gtactgggcg gccaggtctt ccgcttccag cagcgccggc atcagctctc
     1021 gcttccgaga tacgcgcgga tgacgtcggg atcgcgctgg atcgccgcgg gcggtccctc
     1081 cgcgatgcgg cgaccgaagt cgagcaccac cacgcggtcg gacacctgca tgaccaggct
     1141 caagtggtgc tcgaccagga ggacggtcac cccgcgccga tcccggatcg agcggaggat
     1201 gtcgccgagc gcggcgacct cctcgtgatt caggcccccc gccggctcgt ccacgagcag
     1261 cagcttgggg ttgcccgcga tcgcccgcgc cagctccacc cgcttgaggg tgccgaacgg
     1321 aagcccggcc gcgggatgtc cgatcaccgc gtcgagctgg agaaaggcga tggcctccag
     1381 cgcggcctcg cgcatcatcc gctcctcgcg cttgaccgag gggaggcgca gcgcgttgct
     1441 gaggaaatcg ctgccggtgc gcgcgtgcac gccgaccatc acgttctgca gcacggtcat
     1501 gctggggaag agggccaggt tctggaacgt ccggccgatg ccgagcgcgg ccacgtggtg
     1561 cggcgtgttc ttgatgatgg acttgccctc gaagagcaga tccccgctgc tgggcgtgta
     1621 gaggcggctc acgcagttga acagcgtggt cttgcccgcc ccattcggac cgatgagccc
     1681 aacgatgtgg cccggagcca tgtcgaagga gacgtcgtcg agggcgacga tacccccgaa
     1741 gcgcatcgag atgtttcgaa cggacaggat gtcggcaccc acggccggcg tcacggttca
     1801 gtacttatag gggtgggtcc cgcttttgtc aatcctaaac gccacttcgc gagtataatt
     1861 tcgcctctat ggcgcaccaa tacatctacg tgatgaaaga tctgaagaag atcgtcccgc
     1921 ccaagcggga gatcctgaag ggcatctggc tgtcgttcta tcccggggcc aagatcgggg
     1981 tcatcggcgg caacggcgcg ggcaagagca ccctgcttcg catcatggcc ggcgtggaaa
     2041 aggagttcct cggagaggcc tggcccgccg acggggtgcg ggtggggtac ctgccgcagg
     2101 agccgcagct cgaccccgcg aaggatgtgc gcggtaacgt cgaggacggg gtcggggagg
     2161 ttcgctcgct gctgacccgt ttcgacgaga tcaatgcccg cctcggcgag cccctcggcg
     2221 acgacgagat ggaaaaggta ttggccgagc agtcccgggt ccaggacgcg atcgaggcgg
     2281 ccaacggctg ggacctcgac cggaccgtcg agatcgcgat ggacgcgctc cgctgcccgc
     2341 cgggcgacgc ggaggtgagc accctctcgg gcggcgaagt gcggcgggtg gccctctgcc
     2401 gactcctgct ccagaagccc gacctgctgc tcctcgacga gcccaccaac cacctggacg
     2461 ccgagagcgt ggcctggctc gagcggttcc tgaaggacta cccgggcacg gtggtggcca
     2521 tcacccacga ccgctacttc ctcgacaacg tcgcgggctg gatcctcgag ctggatcgcg
     2581 gcgcgggcat cccgtgggag ggcaactact cctcctggct cgaccagaag aagcagcgcc
     2641 tggccatcga ggagaagcag gagaccgcgc gccagcgcac cctcgagcgc gagctcgagt
     2701 gggtgcgcat gtcgccgcgc gcccggcagg ccaagtccaa gtcgcgcctg caggcctacg
     2761 aggagctgct cgccgagggc gggcgcgatc gcgaggggca ggccgagatc atcatcccga
     2821 cgccctcgcg cctgggcgac ctggtggtgc agaccgagca cctggtcaag ggctacggcg
     2881 acaagctcct gatcgaggac ctcacgttca gcctgccccg cggcggcatc gtgggggtca
     2941 tcggccccaa cggagcgggc aagaccacgc tgttccgcat gatcaccggt caggagaagc
     3001 cggaccgggg cgcgctgcag gtgggggaga ccgtccgcct ggcctacgtc gaccagagtc
     3061 gcgacgcgct cgacccggcc aagaacgtgt ggcaggagat ctccggcggg gcggagcagc
     3121 tccagctcgg cacgcgtcag atcgcctcgc gcgcctacgt ggcctcgttc aacttcaagg
     3181 ggtcggacca gcagaagcgg gtgggcgacc tctcgggcgg cgagcgcaac cgcgtgcacc
     3241 tcgccaccat gctcaagagc ggcggcaacc tgctgctcct cgacgagccc accaacgacc
     3301 tcgacgtgga caccctgcgc gccctggaag acgcgctgct ctcgttcgcg ggctgcgcgg
     3361 tggtgatcag ccacgaccgg tggttcctca accgggtggc cacccacatg ctcgccttcg
     3421 agggcgacag ccgggtggtg tggttcgagg gtaactacga ggactacgag gccgaccggc
     3481 acaagcgcct gggcgcggcg gccgacaccc cgcaccggat ccggtacaag ccgctcacac
     3541 gcggctagcg tcgatctccg catcgaggag gaagatccca tgttcaagga catcgtggcg
     3601 tcccgcgagg agctgcgggc gctcttcggc gcccccagcg agcgtgcggt gcagaaggac
     3661 cgcgggcggc tcgacgcgca cacccgcgcg ttcatcgccc ggtccccctt cgtgctgatc
     3721 gccaccgcgg gggcggcggg ccgctgcgac gtctccccca agggcgacgc gcccggcttc
     3781 gtgatggttc tggacgacac ccacctcgcg atcccggacc gggtcggcaa caaccgcatc
     3841 gacggcctca ccaacatcgt ggagaacccg cagctcggac tgatcttcct gatccccggc
     3901 cgcgaggaca ccctgcgcgt gaacggccgc gcctgcatcg tccgcgacga ggagatcctg
     3961 tcccggctgg aggtgcaggg gaagcggccc aaggtggcga tcggcgtcga gatcgaggag
     4021 tgcttcctgc actgcgcgaa ggccttcaag cgctccggcc tctgggatcg ggagcgctgg
     4081 cccgacgtgg cctcactgcc ctcgatggcg aagatcctct gggagcagat cgacctgcag
     4141 gccaccgccc agtgcagcgt ggacgacttc gagcgcgaca tggaggaccg cctccgcaag
     4201 ggcctctatt gatcgacccg tcgcccgccc gggcgcgatc gtgagcccgg gccttccccg
     4261 cgcggccgac atcgtcgtga tcggggccgg cgccatcggc gccagcatcg cgtaccacct
     4321 gggccggcgc ggggcccgcg acgtggtggt gctcgagcgc gacgcggtgg gcgcgggctc
     4381 caccagcaag gcggccgggg gcatccgggt ccagttcgcg acgcgggtgg agatcgagtt
     4441 ctcgcagcgc ggcatcgcgt tcttccagcg cttcgaggac gagatggggg tgccctgcga
     4501 cttccaccag gagggctacc tcttcgtcgt caccgacgag cccaccctca cccgcttccg
     4561 cgccaacgtg gcgctccagc ggagtctcgg agcggacgta cggatcatca gccccgacga
     4621 cgcgcgcgcc ctcgtgccgt cgctcgcgat ggacgacgcg ctcgccgcgg tgtgggggcc
     4681 gaccgacggc cacgcgtcgc ccaacgacgt ggtgcaggcc tacgcggccc gggcccgcgc
     4741 gctcggcgtg cgcatcctcg aggacacgcc ggtgaccggg atccggctgg agggcggccg
     4801 ggtggccgcg gtcgagacgc cggcggggcc catcgccacg cgcctggtcg tcaacgcggc
     4861 ggggccgtgg gccccgctgg tcggccgcat ggccggcctc gacctcccgg tggatccccg
     4921 ccgtcgccac atcttcgtga ccgacgcctt cgacggcatc cgccatccga tgccgctcgt
     4981 caccgacacc gcgagcggct tctactgccg gagcgagcag ggcgcggtgc tgatgagccc
     5041 gggcgacgtc ggggcctcca ccgcctacga ggcccaggtc gactggagcg tgctcgaggc
     5101 cgcggtggag aaggcggtcc gtcgcgtccc ggccctcgag cgggcgcagg tgcgtcacgc
     5161 ctgggccggg ctgcgcccgc tcaccccgga cggacgggcg atcctcgact gggcccccgg
     5221 cgtcgacggt ctctacctcg cggtgggctt ctgcggccac ggattccagc actcgcccgc
     5281 ggtgggcgag acggtggcgg agctcttgct ggaagggcgc accgcggtcg acctgtcgga
     5341 cctgcgcctc ggccgattcg cggttgcttg acaccgccca accggaccgc taacgtcacg
     5401 gttccggcct ccggcatccg cccggaccgg tgaagtagcg catcgaagga ggtgccggcc
     5461 ttgccggatg tctcactcgc ccgcctgcgc caggtggcga gcctcgcccg ctccgtggct
     5521 gggactctcg acgccgacgc ggtgctgcgc gaggtcgtgg aggcgctgcg gaccctgcgt
     5581 cccggcgcct tctgcgtggt ccggctcgtg gatgcggagc gcggcggcta ccggctcgcc
     5641 gcgatgagcg ggagcgatcc gcacgcggtg gcggccgtca tcccgttcgg ccacggcctc
     5701 acccacgcag tggctcagag cggccacccg atgctcctgt ccgacgtgag cggggacccg
     5761 cgcctggtgt cggcgaagtg gcgggaggac tccgggctca aggcctacta cggcgtcccg
     5821 atcaccgcgg ggggcgagct gatgggcgtg ctgagcgtgg cgctcgccga ccatgatgag
     5881 ccgaccgcgg aggagcgcga gatcgtcgac ctgctggcga gccacgccgc ggttgcgatc
     5941 ggcaacgcgc ggctcttcgc ggagagcgag acgcggcgcc gcgcggccga ggccctgtcc
     6001 gaggtgtggc gcttcctgtc cgagaccttt gacgtggccg cgctcgggca gcggatcgcc
     6061 gacgtggtgc tgcgcgcgct ccaggtccgc gccgccgctc tttaccgact ggtaccggcc
     6121 tccggcgacc tggaggtgat cgggcgctcc ggggaagccg agcggttcct cgatccgggc
     6181 ttcgtgatcc cggccggcta cggcatggtg ggcctggccg tgaatcgccg gcaaccggtg
     6241 gtcacccccg acatccgggc ggacgaccgg gtgaagcgca ccccgggcat gcaggtccac
     6301 cacgcgatct gcccgtcggt gctgtcgctg ccgctggtgg tgaaggggca ggtggtgggc
     6361 ggcttcacgg tcggggacac gctcgggcgg gcgtactcgg acgacgacct ccgcctggcc
     6421 cagaccttcg cggaccaggc cgcggtggcg ctcgagaacg cccggctctt cgaggaggcc
     6481 gagcggaggc gatggcagag cctcgccctg ggcgcggtga gccagaccct ggccgagtcg
     6541 ctgcatccga gcggggtggc cgagcgagtg gtggacgcgg cgcgcgaact gctcggcgtg
     6601 cgggtcgcga ccctctatca cctagacccg atcaccggag cgctcgagga gagcgcgtgc
     6661 gccggccgcc tgcccggccg cgcgtggtcg ttgcgccatc cgcgcggcac cggagcggtc
     6721 gggcttgccg ccgcggagcg acgggccgtc gtgtccaacg acgtgctgct cgacccgcgg
     6781 ctcacgttcg ccccggagat gcgcgcccac atcgagggga ccgggctccg cgcggtgatg
     6841 g
//
LOCUS       DATMCV010000526         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_363429, whole genome shotgun sequence.
ACCESSION   DATMCV010000526 DATMCV010000000
VERSION     DATMCV010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363429"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VNU03_12140"
     CDS             <1..404
                     /locus_tag="VNU03_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWP75260.1"
                     /translation="YVAVANAAGFDGVYSYFGHSAIIGFDGRTLGECGEEDYGIQYAA
                     LSKALIRDARKNLQSENHLFKLLHRGYTGKINSREGNEGVAACPYDFYTKWINDPDGT
                     KKMVEALTRSAVGTEECPIPGIPNSKDLTHA"
     gene            429..1238
                     /locus_tag="VNU03_12145"
     CDS             429..1238
                     /locus_tag="VNU03_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HWP75261.1"
                     /translation="MSQPHPIGQYLIRAEPYYRPTGDEVELYRAAYAVRMPMMLKGPT
                     GCGKTRFVEYMAWHLGRPLITIACHEDMTASDLVGRHLLDASGTRWQDGPLTLAVRFG
                     AICYLDEIVEARQDTTVVLHPLADARRILPLEKKAELVEAHPDFQLVISYNPGYQSII
                     KDLKQSTKQRFGALDFGWPPRDAEIEIVVHESGVTADLAGRLVSVGERARNLKGHGLD
                     EGLSTRMLVYAGALIAQGVEPLVACRAALVGPVTDDPDIRDALDAVIKTFF"
     gene            1271..>2498
                     /locus_tag="VNU03_12150"
     CDS             1271..>2498
                     /locus_tag="VNU03_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWP75262.1"
                     /translation="MSFSAIERRLTCYLRALWGRAPAFRTAGRRARVSIVAGAIRMPD
                     LFPAEAGEGIALHRAAAAHAAAHLVYSTHRFPLAKLRPIQVAIVSLIEDARIERLAWR
                     DLPGLRRLWLPFHVARPEAIPNATSLMARLARALIDPDYRDLDGWVAKGRELFEAQDA
                     RLHDPSISREIGTLLGNDLGQMRMQFDYGHYLVEPAYRDDNAFLWEFPENPSTPPPES
                     GPAVLAGTVTLEADGTPEGDRADQSPDGARREISPPQAVPATEVPDAPPHRYPEWDYV
                     IGVHRPAWCTVRERRREPGDAGAIDEILLRHEEVVARVTRLVRSAQVGHAARLRRQVE
                     GNDLDLDAAIAAIADLRAGRTPSPRVYQRPGRIRRDLAVLWLLDLSRSTNDVVSGTGE
                     TILALARAATAVVAEAI"
BASE COUNT          365 a          930 c          841 g          362 t
ORIGIN      
        1 tctacgtggc ggtggccaac gcggcgggct tcgacggcgt ctactcctac ttcggccact
       61 ccgcgatcat cggcttcgat ggccgaaccc tcggcgagtg cggcgaggag gactacggca
      121 tccagtacgc ggcgctctcc aaggccttga tccgggatgc gcgcaagaac ctccagtccg
      181 agaaccacct gttcaagctc ctccaccgcg gttacaccgg caagatcaac tcgcgcgagg
      241 gcaacgaggg ggtggccgcc tgcccgtacg acttctacac gaagtggatc aacgatcccg
      301 acggcaccaa gaagatggtc gaggcgctca cccgctcggc ggtcggcacc gaggagtgcc
      361 cgatccccgg catcccgaac agcaaggacc tcacgcacgc ctaggtcggc gcgcagtcgc
      421 tcgcggaggt gagccagccg catccgatcg gccagtacct gatccgggcc gagccgtact
      481 accggccgac cggtgacgag gtcgagctct atcgggccgc gtacgcggtg cgcatgccga
      541 tgatgctcaa ggggccgacc gggtgcggca agacgcgctt cgtcgagtac atggcgtggc
      601 acctcggccg gccgctgatc accatcgcct gccacgagga catgaccgcc tccgatctgg
      661 tcgggcggca tctcctcgac gcgagcggga cccggtggca ggacgggcct ctcaccctcg
      721 ccgtccgctt cggggcgatc tgctatctcg acgagatcgt ggaggcgcgc caggacacca
      781 cggtggtgct ccacccgctg gccgacgcgc ggcggatact tccgctcgag aagaaggccg
      841 agctggtgga ggcgcatccc gacttccagc tcgtcatctc ctacaacccc ggctaccaga
      901 gcatcatcaa ggatctcaag cagtccacca agcagcgctt cggcgccctc gacttcggct
      961 ggcccccgcg cgacgccgag atcgagatcg tggtccacga gtccggcgtg accgccgacc
     1021 tcgccgggcg gctcgtgagc gtcggcgagc gggcccgcaa cctcaagggc cacggcttgg
     1081 acgaggggtt gtccacccgc atgctcgtct acgcgggcgc cctgatcgcg cagggggtgg
     1141 agccgctcgt cgcgtgccgg gccgccctcg ttggtccggt caccgacgat ccggacatcc
     1201 gggacgccct cgacgcggtg atcaagacct tcttctgagc gcggtggcgc cgacggcatc
     1261 gtccacggcc gtgtcgttct cggcgatcga gcggcgcctc acgtgctacc tgcgcgcgct
     1321 gtgggggcgg gcccccgcgt ttcgcaccgc cggccggcgc gcgcgcgtct ccatcgtcgc
     1381 gggcgccatc cgcatgcccg atctctttcc tgcggaggcc ggagagggca tcgcgctcca
     1441 tcgcgccgcc gccgcgcacg cggccgcgca tctcgtctac tcgacccacc gcttcccgct
     1501 cgccaagctg cgtccgatcc aggtggcgat cgtctccctg atcgaggacg cccggatcga
     1561 acggctcgcg tggcgggacc tgcccgggct gcgccggctc tggctgccgt ttcacgtcgc
     1621 ccggccggag gccatcccga acgcgacctc cctcatggcc cgcctggccc gcgccctcat
     1681 cgacccggac tatcgagacc tcgacggctg ggtcgccaag ggccgcgagc tgttcgaggc
     1741 tcaggacgcg cggttgcacg atccttccat cagccgcgag atcgggacct tgctcggcaa
     1801 cgatctcggc cagatgcgga tgcagttcga ctacgggcac tacctggtcg agcccgccta
     1861 ccgcgacgac aacgcgttcc tgtgggagtt ccccgagaac ccatccaccc ctccgccgga
     1921 gagcgggccg gcggtgctcg cgggcaccgt gacgctcgag gcggatggca cgcccgaggg
     1981 cgaccgcgcg gaccagagtc cagacggagc gcgccgggag atctcgccgc cgcaggcggt
     2041 gcccgcgacg gaggtgcccg acgcgccgcc ccatcgctat ccggagtggg actacgtcat
     2101 cggcgtccac cgccccgcct ggtgcacggt ccgcgagcgg cgccgcgagc cgggcgatgc
     2161 cggtgccatc gacgagatcc tgttacgcca cgaggaggtg gtggcgcggg tcactcgcct
     2221 cgtccggagc gcgcaggtcg gccacgccgc gcggctccga cgccaggtgg aaggcaacga
     2281 tctcgacctc gacgcggcca tcgccgccat cgccgatctg cgcgccggcc gtacgccgag
     2341 cccgcgggtc taccagcgcc cggggcgcat ccggcgcgat ctcgcggtgc tctggctgct
     2401 cgatctctct cgctccacca acgacgtcgt gtccggcacg ggcgagacca tcctggccct
     2461 cgcccgcgcg gccaccgcgg tggtggcgga ggcgatcg
//
LOCUS       DATMCV010000527         6036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_26195, whole genome shotgun sequence.
ACCESSION   DATMCV010000527 DATMCV010000000
VERSION     DATMCV010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26195"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1016)
                     /locus_tag="VNU03_12155"
     CDS             complement(84..1016)
                     /locus_tag="VNU03_12155"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75263.1"
                     /translation="MRVGFIGLGIMGASMASNLQAAGHALAVHDVRRAAAGPHLSAGA
                     VWKDTPRALAEDVDVVFTSLPGPPEVEQVALGADGLIHGMRAGTALFDLTTNAPALVR
                     RLHGLFAARGVQVLDAPVSGGPVGAKTRKLAIWVGGDREVFDRLRPVLEAMGDQPYYV
                     GPIGAGAVAKLVHNCASFTIRTVLAEVFSLGVKAGVAPELLWQAVRQGATGRRRTFDV
                     LVDKFLPGEFEPPSFALRLGHKDVSLATALGREHGVPMRLANLALEEITEALNRGWGD
                     RDSSSFMLLEEERAGVQIAVPRERLQQILEQDGR"
     gene            1117..2004
                     /locus_tag="VNU03_12160"
     CDS             1117..2004
                     /locus_tag="VNU03_12160"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP75264.1"
                     /translation="MAPTFRKLHPCFAAEVGPVDLKTVHDEATLGRIRAGMDEFAVLV
                     FRDQVWSDAEHLDFAQRLDGRLHTKLGISALQKNRLGNEALGDISNLDESGEIMKSDN
                     RKRMYGLGNRLWHTDASFQDPPGRYSMLSAKVVPPVDADTEFADMRAAYDALSEAERA
                     RLEGLRVHHSIAYSRQTLGFEFSENEQEALKGAVHPLVRTIPRSNRRSLYLASHASRI
                     IDWPVPEGRLLLRELIEHATQPRFVYRHQWRVGDLVIWDNRATMHRARPFDDARYRRE
                     LRRVTTLDVEQPAAVTTGV"
     gene            2048..3688
                     /locus_tag="VNU03_12165"
     CDS             2048..3688
                     /locus_tag="VNU03_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HWP75265.1"
                     /translation="MRTPYEPRHRRRILCISPRYAPSFGTFHYSYPLFGGRIRAFMPP
                     QGLLVVAAYLPESWEVRFVDENSRPATAEDLAWADAVFLSGMHVQRPFIEDAIARAHH
                     AGKPVVLGGPSVSGCPEHYPGADLIQVGEMGDSTDSIIRWLDEHAGRPAAQLRFDTTT
                     RLPLTDFPLPAYHLIRLPQYFISSVQFSSGCPYSCEFCDIPALYGRNPRLKTPGQVLA
                     ELDALVAGGAVAIYFVDDNFIANQKAALELLPHLVEWQRRHRFPVRFACEATLNIAKN
                     ERVLGLMRDAGFHVVFCGIETPEPDALRSMSKDQNLRMPILDAVRKLNDYGLEVVSGI
                     ILGLDTDTPATADHLIEFIEASQIPMLTINILYALPRTPLWDRLAAEGRILPQGGRES
                     NVVFRLPEETVIGMWRRAIGAAYAPEAIYRRFAHNLAHTFSKRPAYPRNPRRASMRNA
                     VSGLALLGRIVWRVGVRGDYRRTFWRVTGQALRRGQFEELIQAAVVSYHLIEFTRQCL
                     RGAHEASFYAPRVPAAPPTPVPPLRLPRPGLEREGASR"
     gene            3712..5091
                     /locus_tag="VNU03_12170"
     CDS             3712..5091
                     /locus_tag="VNU03_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HWP75266.1"
                     /translation="MSNAPDRPAVVRQDLDNVIHPIVPHKQLEQQQLVIVSAKDSTVV
                     DADGREYLDGMAGLWCVNIGYGRTELAEVAADQMRDLAYYPHTAMNRPAAALAEKVNG
                     LLGGDNHVYFVSSGSEANEAAFKFARQYARHEFPQQNRYKTISRYYAYHGTTLATLAA
                     GGMGDRKMKFEPLSGNDFVHVAPPYCYRCPFGLEPTSCELACVKNMETTIQGEGPETV
                     AEVIIEPIMSAVGVAVPPDGYLPAVAELCKKHGVLLHVDEVINGFGRTGRLFAHEHAG
                     IKPDIVTVAKGISSAYLPMAATVVRNSVFQSFLGDPSENRQVNQVNTYGGHPVAAAVA
                     VRNIEILLQEKLAERSAENGAYLLDGLRTLTRHKWVGDVRGKGLYAGVELVTDRASKA
                     VMPQAQIKRITERCQELGVIVGRSGGGRHLGNTLVLAPPLVLTRPEIDRIVSVLDRVI
                     PEICPEATA"
     gene            5088..>6036
                     /locus_tag="VNU03_12175"
     CDS             5088..>6036
                     /locus_tag="VNU03_12175"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HWP75267.1"
                     /translation="MSATFPHHVLRGSHREVGRLHGEALRPMIRAHLELIYTQGAQRS
                     KLAPETARRWALAFGPMIGEAAPHFLDEIEGVAEGAGIELAEALLLQLRQEVAHVARF
                     GTVDLECTSFAVSGPYTRTGGTLAGQNADLAGGIEAFSAVLTFAVTGKPAVMMLVPAG
                     QISYLGMSSEGLSADANFLRSAGWRTGFPRYLLTRLAIEQPTLKAAVDAALTPRRASS
                     RNLLLADRAGAMVDIETTAREHGLAWGDGCLVHANHHVVPGMAAHETANADELHNSTC
                     RQERIAALMETHRGRLDAETLKTVLRDHANRPHSICAHPG"
BASE COUNT          878 a         2332 c         1997 g          829 t
ORIGIN      
        1 gccgcctgga cggtcttacc tcctcttcac tcctaccggc ggccctggag gcacctggcg
       61 atctcgcgcg cgcgccgggc gcctcaccgc ccgtcctgct ccaggatctg ctgcagccgc
      121 tcgcgcggca ccgcgatctg gacccccgcc cgctcctcct cgagcagcat gaagctgctc
      181 gagtcccggt cgccccagcc gcgattgagc gcctccgtga tctcctccag cgccagattc
      241 gcgaggcgca tcggcactcc gtgctcacgc ccgagcgcgg tggcgaggga gacgtccttg
      301 tggccgaggc gcagcgcgaa ggacggcggc tcgaactcgc cgggcaggaa cttgtcgacc
      361 agcacgtcga acgtgcggcg gcggccggtc gcgccctgcc gcaccgcctg ccacagcagc
      421 tcgggcgcca cccccgcctt cactcccagg ctgaacacct cggccagcac ggtgcggatc
      481 gtgaagctgg cgcagttgtg cacgagcttg gcgaccgcgc ccgccccgat cgggcccacg
      541 tagtagggct ggtcgcccat cgcctcgagc accggccgca ggcggtcgaa cacctcgcgg
      601 tcgccgccca cccagatcgc gagcttccgg gtcttcgccc cgaccgggcc cccgctcacc
      661 ggcgcgtcga gcacctggac cccgcgcgcc gcgaagagcc cgtgcaggcg ccggaccagg
      721 gccggggcgt tggtggtgag gtcgaagagc gcggtgcccg cgcgcatgcc gtggatgagc
      781 ccgtccgccc cgagggccac ctgctcgacc tcgggcggcc cggggagcga ggtgaacacc
      841 acgtccacgt cctccgcgag ggcccgcggg gtgtccttcc agaccgcgcc cgcggacagg
      901 tgtggccccg ccgcggcccg gcgcacgtcg tgcaccgcga gcgcgtgacc cgccgcctgg
      961 agattggacg ccatgctggc gcccatgatg ccgagaccga tgaacccgac tctcatgacg
     1021 aggttcctcc cggccggcat cttggcacag gcacccgacc gtttgcttga ctcccaggcc
     1081 cgcccggcct agattcggtc ccgggaggcg agcccaatgg cgccgacatt ccgcaagctt
     1141 cacccctgtt ttgccgccga ggtcggcccg gtcgacctca agacggtcca cgacgaggcc
     1201 acgctcgggc gcatccgcgc cggcatggac gagttcgcgg tcctcgtgtt ccgggaccag
     1261 gtctggagcg acgccgagca cctcgacttc gcccagcgcc tcgacggccg gctccacacc
     1321 aagctcggaa tcagcgcgct ccagaagaat cgcctgggca acgaggcgct cggcgacatc
     1381 tcgaacctcg acgagagcgg cgagatcatg aagtcggaca accgcaagcg catgtacggg
     1441 ctcggcaacc ggctctggca caccgacgcc tcgttccagg acccacccgg ccgctactcg
     1501 atgctctccg ccaaggtggt gccgccggtc gacgcggaca ccgagttcgc cgacatgcgc
     1561 gccgcgtacg acgccctgtc cgaggcggag cgggcgcgcc tcgagggtct gcgcgtgcat
     1621 cactcgatcg cctactcgcg ccagaccctc ggcttcgagt tctccgagaa cgagcaggag
     1681 gcgctgaagg gcgcggtcca tccgctggtg cggacgatcc cgcgctccaa ccggcgctcg
     1741 ctctacctgg cctcgcacgc ctcgcggatc atcgactggc cggtcccgga aggccggctg
     1801 ctcctccgcg agctgatcga gcacgcgacc cagccgcgct tcgtgtaccg gcaccagtgg
     1861 cgcgtcggcg acctcgtgat ctgggacaac cgcgccacca tgcaccgcgc gcgcccgttc
     1921 gacgacgcgc gctaccggcg cgagctgcgg cgcgtgacga ccctcgacgt ggagcagccg
     1981 gcggccgtga ccaccggggt ctgaccggcc ccctcccgtc gcctcccggc ctcgacccgc
     2041 ccgcccgatg cgcacgccct acgagccccg acaccggcgg cggatcctct gcatctcgcc
     2101 gcgctacgcg ccgtcgttcg ggacgttcca ctactcctac ccgctcttcg gcggccgcat
     2161 ccgggccttc atgccgccgc aggggctgct ggtggtggcc gcctacctgc cggagtcgtg
     2221 ggaggtgcgc ttcgtggatg agaactcgcg gccggccacc gcggaggacc tcgcgtgggc
     2281 ggacgcggtc ttcctctccg gcatgcacgt gcagcgcccg ttcatcgagg acgcgatcgc
     2341 gcgggcccac cacgccggca agccggtcgt gctcggcggg ccctcggtgt cgggctgccc
     2401 cgagcactac ccgggcgcgg acctgatcca ggtcggcgag atgggcgatt ccaccgactc
     2461 catcatccgc tggctcgacg agcacgccgg gcgcccggcc gcccagctcc gcttcgacac
     2521 cacgacgcgg ctgcccctca ccgacttccc gctccccgcc taccacctga tccggctgcc
     2581 gcagtacttc atctcgagcg tccagttctc gagcggctgc ccgtactcgt gcgagttctg
     2641 cgacatcccc gcgctctacg gccgcaatcc gcggctcaag acccccgggc aggtgctggc
     2701 cgagctcgac gccctcgtcg cgggcggcgc ggtcgccatc tacttcgtgg acgacaactt
     2761 catcgcgaac cagaaggcgg ccctcgagct cctcccccac ctggtggaat ggcagcggcg
     2821 tcatcggttc ccggtgcgct tcgcctgcga ggcgacgctc aacatcgcca agaacgagcg
     2881 cgtgctcggc ctcatgcgcg acgccggctt ccacgtggtg ttctgcggca tcgagacccc
     2941 ggagccggac gcgctccggt cgatgtccaa ggaccagaac ctccgcatgc cgatcctcga
     3001 cgcggtgcgg aagctcaacg actacggcct cgaggtggtc tccggcatca tcctcgggct
     3061 cgacaccgac acccccgcga ccgccgatca cctgatcgag ttcatcgagg cctcccagat
     3121 cccgatgctc acgatcaaca tcctctacgc gctgccccgg accccgctct gggatcggct
     3181 cgccgccgag ggccgcatcc tgccccaggg cgggcgcgag tccaacgtgg tgttccgcct
     3241 gcccgaggag accgtgatcg gcatgtggcg ccgagccatc ggggcggcct acgccccgga
     3301 ggcgatctac cggcgcttcg cccacaacct cgcgcacacg ttctcgaagc gcccggccta
     3361 tccgcgcaac ccccggcgcg cgtcgatgcg caacgcggtg tccgggctgg ccctgctcgg
     3421 acgcatcgtc tggcgggtcg gagtgcgggg cgattaccgg cgcaccttct ggcgggtcac
     3481 cggccaggcc ctgcgccgcg gccaattcga ggagctgatc caggccgcgg tggtgagcta
     3541 tcacctgatc gagttcaccc gccagtgcct ccgcggagcc cacgaggcct cgttctacgc
     3601 cccccgcgtc ccggccgcgc cgccgacgcc cgtgccgccg ctccgcctgc cgcggcccgg
     3661 cctcgagcgc gagggcgcgt cccgctagcg cgggaagaca ggagactccc catgtcgaac
     3721 gcccccgacc ggcccgcggt ggtccggcag gacctggaca acgtcatcca cccgatcgtg
     3781 ccccacaagc agctcgagca gcagcagctc gtcatcgtct ccgccaagga ctccaccgtg
     3841 gtggacgccg acggccgcga gtacctcgac ggcatggccg ggctctggtg cgtgaacatc
     3901 ggctacggcc gcaccgagct ggccgaggtg gcggccgacc agatgcggga tctcgcctac
     3961 tacccgcaca ccgcgatgaa ccggcccgcc gccgcgctcg ccgagaaggt gaacgggctg
     4021 ctgggcggcg acaaccacgt ctacttcgtc agctcgggct cggaggccaa cgaggccgcc
     4081 ttcaagttcg cgcggcagta cgcgcggcac gagttccccc agcagaaccg ttacaagacg
     4141 atcagccggt actacgccta tcacggcacc acgctcgcca ccctcgccgc gggcggcatg
     4201 ggcgaccgca agatgaagtt cgagccgctc tccggcaacg acttcgtcca cgtggccccg
     4261 ccctactgct accgctgccc gttcggcctc gagccgacga gctgcgagct cgcctgcgtc
     4321 aagaacatgg agaccaccat ccagggcgag gggccggaga cggtggccga ggtgatcatc
     4381 gagccgatca tgagcgcggt cggcgtcgcg gtcccgccgg acggctacct gcccgcggtg
     4441 gccgagctct gcaagaagca cggggtcctc ctgcacgtcg acgaggtcat caacgggttc
     4501 ggccggaccg gccggctctt cgcccacgag cacgcgggga tcaagcccga catcgtcacg
     4561 gtggccaagg gcatctcgag cgcctacctg ccgatggcgg ccaccgtggt ccgcaactcc
     4621 gtcttccagt ccttcctcgg cgatccgtcg gagaaccggc aggtgaacca ggtcaacacc
     4681 tacggcggcc atccggtggc ggccgcggtg gccgtgcgga acatcgagat cctgctgcag
     4741 gagaagctcg ccgagcgctc cgccgagaac ggcgcctacc tgctcgacgg gctccggacc
     4801 ctcacccgcc acaagtgggt gggcgacgtg cgcggcaagg gcctctacgc gggcgtggag
     4861 ctggtgaccg accgcgcctc gaaggcggtg atgccgcagg cccagatcaa gcgcatcacc
     4921 gagcgctgcc aggagctggg ggtgatcgtg ggccgctcgg gcggcggccg ccatctcggc
     4981 aacaccctcg tcctcgcgcc gccgctggtc ctcacccgcc ccgagatcga ccgcatcgtg
     5041 tcggtgctgg accgggtgat ccccgagatc tgcccggagg ccacggcatg agcgcgacgt
     5101 tcccgcacca cgtcctgcgc ggcagccacc gtgaagtcgg tcggctgcac ggcgaggccc
     5161 tgcgtcccat gatccgggca cacctcgagc tgatctacac ccagggggcc cagcgctcga
     5221 agctcgcgcc ggagaccgcc cgccgctggg ccctggcctt cgggcccatg atcggcgagg
     5281 ccgcgcccca cttcctggac gagatcgagg gcgtggccga gggggccggg atcgagctcg
     5341 cggaggcgct cctgctgcag ctccgccagg aggtcgcgca cgtggcccgc ttcggcaccg
     5401 tggacctcga gtgcacgagc ttcgcggtct cgggccccta cacccgcacc ggcgggaccc
     5461 tcgcgggcca gaacgcggac ctcgcgggcg gcatcgaggc cttcagcgcg gtgctcacct
     5521 tcgcggtcac cggcaagccc gcggtgatga tgctggtgcc ggcggggcag atcagctatc
     5581 tcgggatgag cagcgagggc ctctcggccg acgccaactt cctgcgctcg gcgggctggc
     5641 gcaccggctt cccgcgctac ctgctgaccc ggctcgccat cgagcagccg accctcaagg
     5701 ccgcggtgga cgcggcgctg accccgcggc gcgcgtcgtc acgcaacctg ctgctggccg
     5761 accgcgcggg cgccatggtc gacatcgaga ccaccgcgcg ggagcacggg ctcgcctggg
     5821 gcgacggctg cctggtgcac gccaatcacc acgtggtccc cgggatggcc gcgcacgaga
     5881 ccgcgaacgc cgacgagctc cacaactcca cctgccgcca ggagcgcatc gcggcgctga
     5941 tggagacgca tcgcgggcgg ctcgacgcgg agacgctcaa gacggtgctg cgcgaccacg
     6001 ccaaccggcc tcactcgatc tgcgcgcacc cgggcg
//
LOCUS       DATMCV010000528         2131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_95948, whole genome shotgun sequence.
ACCESSION   DATMCV010000528 DATMCV010000000
VERSION     DATMCV010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95948"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VNU03_12180"
     CDS             <1..1052
                     /locus_tag="VNU03_12180"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl reductase"
                     /protein_id="HWP75268.1"
                     /translation="GAHVSAIRGDVSVPEEVERILADVRREMPPLRGVIHAAGVLADA
                     TVRETTADGIKAVMAPKAAGAWALHGATATDPLDFFVLFSSASALLGLPGQANYAAAN
                     AFLDGLARHRRELGRPALSIAWGPWAGAGLAAERADRGERLAGQGLGSLGAAQGVEAF
                     RRVLGRDRAYVAVMAFDPDAWCEAQPGAPARLFERMRTAGPSVAEDRAANGILGALRE
                     SPAGARPTMLLEHLRGRVARVLRLEPAHVHADAPLKSLGLDSLMALELRNRLEADLSL
                     RLSATLVWSHPTISALVTHFVEVLDLGAAGPSVEDTPRAAEPELSVAQVAALLDEELS
                     RVDRLLAGGPEGHGG"
     gene            1045..>2131
                     /locus_tag="VNU03_12185"
     CDS             1045..>2131
                     /locus_tag="VNU03_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HWP75269.1"
                     /translation="MAEHEAAQLKQALDALTKMRVRLEAAERAKAEPIAIVGIGCRFP
                     GGAHDADSFWSLVMERRDAVTEVPSDRWDVATFYDPDPLAAGKTSTRWGAFLEGIDRF
                     DAAFFGISPREAARMDPQQRLLLEVAWNALEDAGVSPAGLAGQSAGVFVGVHSHSADY
                     WGLQAHDRGGLDPYAGTGTSHSVLAGRLSYLLDLHGPSLAVDTACSSSLVAVHLACQA
                     LRAGECGLALAAGVNVILTPEFTVAASRMQMLAADGRCKAFDARADGFVRGEGCGVVV
                     LKRLADALAAGDPIRAVIRGSGVNQDGRTNGLTAPSGPSQSALIRSVLTAAGVAGREI
                     GYVEAHGTGTPLGDPIEVEALAAALGDR"
BASE COUNT          275 a          728 c          829 g          299 t
ORIGIN      
        1 agggcgcgca cgtgagcgcg attcgcgggg acgtgagcgt gccggaagag gtcgagcgga
       61 tcctggccga tgtgcggcgt gagatgccgc cgcttcgggg cgtgatccat gcggcgggtg
      121 tcctcgccga cgcgacggtg cgggagacga cggcggacgg gatcaaagcc gtgatggcgc
      181 cgaaggcggc cggcgcgtgg gcgctccacg gagcgaccgc caccgatccc ctcgatttct
      241 tcgtgctgtt ctcgtcggcg tccgcgctgc tgggcctccc cgggcaggcg aactacgcgg
      301 cggccaacgc attcctcgac gggctggccc gtcatcgccg ggaactcggg cggccggcgc
      361 tcagcatcgc ctgggggcca tgggcggggg cgggtctggc ggcggagcga gcggatcgcg
      421 gcgagcggct ggccggacag ggcctgggaa gcctgggcgc cgctcagggt gtcgaagcgt
      481 tccgacgtgt cctcggccgc gaccgcgcct acgtcgcggt gatggccttc gacccggatg
      541 cctggtgtga ggcgcagccg ggagcgccgg cacgactctt cgagcggatg cgcacggcgg
      601 gtccgtcggt agccgaggac cgcgccgcca acgggatcct cggcgccctg cgcgagtcgc
      661 cggcgggcgc gcggcccacc atgctcctcg agcatctgcg gggacgagtc gcgcgcgtcc
      721 tgcgtctcga gccggcccac gtccacgcgg acgccccgct caagagcctg gggctcgatt
      781 cactcatggc gctcgagctg aggaatcggc tagaggccga tctgagtctg cggctctccg
      841 ccacgctggt gtggagccac ccgaccatct cggcgctcgt cacgcatttc gtcgaggtgc
      901 tcgacctcgg agcggccggg ccatcagtgg aggacacccc gcgggcggcc gagccggagc
      961 tctcggtagc ccaggtggcg gcgctcctcg acgaggagct gagccgggtc gatcgactgc
     1021 tcgccggtgg accggaggga cacggtggct gagcacgagg cggcccagct gaagcaggcg
     1081 ctcgacgcgc tgacgaagat gcgcgtgcgg ctcgaggcgg ccgagcgggc caaggccgag
     1141 cccatcgcca tcgtcggcat cggctgccgc tttccgggcg gcgcgcacga tgcggactcg
     1201 ttctggtccc tggtgatgga gcgccgggac gcggtgaccg aggtgccgtc ggaccgatgg
     1261 gacgtcgcga cgttctacga tccagacccg ctcgcggcgg gaaagaccag cacccgctgg
     1321 ggcgccttcc tcgagggcat cgaccgcttc gacgccgcgt tcttcggcat ctcgccgcgc
     1381 gaggccgcgc gcatggaccc gcagcagcgc ctgttgctcg aggtcgcgtg gaacgcgctc
     1441 gaggacgccg gcgtctcccc cgccgggctc gccggacaga gcgcgggagt gttcgtgggc
     1501 gtgcacagcc acagcgccga ctactggggt ctccaggcgc acgatcgcgg cgggctggat
     1561 ccgtacgccg gcaccgggac ctcgcacagc gtgctggccg gtcggctctc gtacctgctc
     1621 gatctgcacg gaccgagtct ggcggtggac accgcgtgct cgtcctcgct ggttgcggtc
     1681 catctcgcct gtcaggccct ccgggcggga gagtgtgggc tcgcgctggc ggcgggcgtc
     1741 aacgtgatcc tcacgcccga gttcacggtg gccgcgtcgc ggatgcagat gctcgcggcc
     1801 gatggtcgct gcaaggcgtt cgacgcgcgg gccgacgggt tcgtccgcgg tgaaggatgt
     1861 ggggtcgtgg tgctgaagcg cctcgccgat gcgctggccg ccggcgaccc gatccgggcg
     1921 gtcatccggg gctccggcgt gaaccaggac ggacgcacga acggactgac cgcgccgagc
     1981 gggccctcgc agagcgccct gatccgatcc gtcctcaccg cggcgggggt ggcgggccgc
     2041 gagatcgggt acgtggaagc ccacggcacc gggacaccgc tcggcgaccc gatcgaggtg
     2101 gaggcgctcg cggcggccct cggggaccga g
//
LOCUS       DATMCV010000529         5050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_295578, whole genome shotgun sequence.
ACCESSION   DATMCV010000529 DATMCV010000000
VERSION     DATMCV010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295578"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VNU03_12190"
     CDS             complement(<1..228)
                     /locus_tag="VNU03_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HWP75270.1"
                     /translation="MPVVGVDHVQLAMPAGREAEARAFYGGLLGIPEIAKPPALAARG
                     GAWFESGRLKIHLGVDRDFRPAQKAHPALLVS"
     gene            complement(228..503)
                     /locus_tag="VNU03_12195"
     CDS             complement(228..503)
                     /locus_tag="VNU03_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009631806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HWP75271.1"
                     /translation="MLYMVIEYFNAGAAPDIYRRARERGRQLPPGLDYVDSWVDLDYF
                     RCFQLMRTDDRSLFDVWMKAWDDLAHFEIIPVRTSAEAARHIADRAG"
     gene            complement(521..907)
                     /locus_tag="VNU03_12200"
     CDS             complement(521..907)
                     /locus_tag="VNU03_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005208505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP75272.1"
                     /translation="MQPQPLIAVRDVPASSRWYQQLLGCASGHGGDEYEQLVDPAGTL
                     ILQLHAWDAHDHPHLGNPDVRPHGNGVLLWFQVGDFDAAVARAGALGAPVLEGPKVNP
                     AARHREIWLRDPDGYVVVLATAYGDL"
     gene            complement(889..1677)
                     /locus_tag="VNU03_12205"
     CDS             complement(889..1677)
                     /locus_tag="VNU03_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWP75273.1"
                     /translation="MLLRLAFLHLAPIAGDLAHNRRLVEDAVTAAAELGAAWVITPEL
                     CITGYTFADTIGTDWIREAPDVWTADLCRTVARLRVTVFLSHPERDARTGALHNSVFV
                     IAPDGRIVGTHRKVNTLPVGSEAWSTPGEQVAPIVVEPVGPVGILVCADAYSPWIAKS
                     LHAQGARLLVSLASWAPGLHGPNGEWEQCTRDTGLPMLVCNRTGRDRVVDFTPGESVV
                     VKDGERLLALHAPDSTIFTVDWDLEAARPAGPPLTHRLECSPSR"
     gene            complement(1686..2525)
                     /locus_tag="VNU03_12210"
     CDS             complement(1686..2525)
                     /locus_tag="VNU03_12210"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWP75274.1"
                     /translation="MGKLDGKVAVITGGASGIGHAAVRLFVEEGCRVVIADLQDDKGT
                     ALAGELGGKSASYLHVDVSREADVSGAIAHAVARFGRLDCVFNNAGAGGVTGPIAEIP
                     AEGFDQTMGVLLRGVFLGMKHAAPIMKQQGGGSIISTASVAGLQAGFGPHVYSAAKAA
                     VVHLTRSVAMELGESGVRVNCICPGGIATPIFGGGLGLAGPEADRIAEMMKTLLVARQ
                     PIQRAGLPEDIARAALWLASDDSAFVNGHALIVDGGLTGGRMWSVVQEQRQQLRRALG
                     LGE"
     gene            complement(2541..3233)
                     /locus_tag="VNU03_12215"
     CDS             complement(2541..3233)
                     /locus_tag="VNU03_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWP75275.1"
                     /translation="MLRPLASLLGLLVALTAAAPAGAQVPPSAAERARYTGLLAAADR
                     GDTAGIRSLTAGGINPDGPDAHGRTPLHVAAHRGHHEAMRALVVAGADPNALDRDRYD
                     IVTIAAVAGDVRTLETALALGASSRSVTSRYDGTALIAAAHLGHADVVRTLIRAGAPL
                     DHVNNLGWTALIESIVLGDGGPRHTDTLTALVTAGADVNLADRGGRTPLALARARGHD
                     TMARVLEQAGAR"
     gene            complement(3347..4120)
                     /locus_tag="VNU03_12220"
     CDS             complement(3347..4120)
                     /locus_tag="VNU03_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75276.1"
                     /translation="MTETGPLYDRALLLAGARRNAVLDLEEVRRYGADSYRDRDYVSI
                     YGLPPADWYARGIRLLGRTAVECTRDDLGDAIGRDVAGIALMAPASEGTRVIDLFAGS
                     GNTLYWMLRHQPGARGLGFELDAAVFGLTRRNLAILGLPIDVANADYRAGLADAAVPA
                     DHLVVVFVAPPWGDALGQTTGLDLRRTTPPIAEIVDVLAARFEGHRILCAIQIHETVE
                     PASLAELTPRFDWSARRIYGLNAPGENHGVLLGTRGWAP"
     gene            complement(4125..4604)
                     /locus_tag="VNU03_12225"
     CDS             complement(4125..4604)
                     /locus_tag="VNU03_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75277.1"
                     /translation="MLDLSGGTAGALMAADRTALAPLFRQVSESTDAPPRSTLLLVYC
                     TIAADGAILNSARSLREIIRDAGAPVVVVATPNSGQSYTVAANRNKQLARANLVMTLD
                     RKGETFAHFFRRLVADMKRGTSMPRAWAKLTPQTPRGTHPDCPDAILACELGQLAFS"
     gene            complement(4695..>5050)
                     /locus_tag="VNU03_12230"
     CDS             complement(4695..>5050)
                     /locus_tag="VNU03_12230"
                     /inference="COORDINATES: protein motif:HMM:NF025457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4269 domain-containing protein"
                     /protein_id="HWP75278.1"
                     /translation="GAFANRPGFACERQPAGATLATLARFRSRELPVEVFGQALPVDL
                     QRGYRHLLVEARLLDLGSPRLRRAIRDLREDGMKTEPAFARALGLRGDPHERLWALSW
                     CDDAELRAILGAGDP"
BASE COUNT          677 a         1799 c         1914 g          660 t
ORIGIN      
        1 gctcaccagc agcgcggggt gcgccttctg cgcggggcgg aagtcgcggt cgacgcccag
       61 gtggatcttg aggcgcccgc tctcgaacca cgcgccgccg cgggcggcga gcgccggcgg
      121 cttggcgatc tcgggaatgc cgagcagccc gccgtagaag gcgcgcgcct ccgcctcgcg
      181 cccggccggc atcgcgagct gcacatgatc gaccccgacg acgggcatca gccggcccgg
      241 tccgcgatgt gccgggccgc ctcggcggaa gtgcgcaccg ggatgatctc gaagtgcgcg
      301 aggtcgtccc acgccttcat ccagacgtcg aacagcgacc ggtcgtcggt ccgcatgagc
      361 tggaagcagc ggaagtagtc gaggtcgacc cagctgtcga catagtcgag cccgggcggg
      421 agctgccgcc cccgctcccg ggcccggcga tagatgtcgg gcgcggcgcc tgcgttgaag
      481 tactcgatga ccatgtagag catgggccag tgtcctcttt tcagagatcg ccgtaggcgg
      541 tggccagcac gacaacgtag ccatccggat cgcgcagcca gatctcgcgg tgcctggcgg
      601 ccgggttgac cttcggtccc tcgaggaccg gcgcgccgag ggcgccggcg cgcgcgaccg
      661 ccgcgtcgaa gtcgccgacc tggaaccaga gcaggacgcc gttgccgtgg ggccggacgt
      721 cgggatttcc caggtgcgga tgatcgtgcg cgtcccaggc gtgcagctgg aggatgagcg
      781 tcccggccgg atccacgagc tgctcgtact cgtccccgcc gtggccgctc gcgcagccga
      841 ggagctgctg gtaccagcgg ctgctggccg ggacgtcgcg gaccgcgatc agcggctggg
      901 gctgcattcg agacggtggg tcagcggcgg acccgccggg cgcgcggcct cgaggtccca
      961 gtccaccgtg aagatcgtcg agtcgggcgc gtgcagcgcg agcagccgct cgccgtcctt
     1021 gaccacgacg ctctcgcccg gggtgaagtc caccacgcgg tcccggccgg tccggttgca
     1081 gaccagcatc gggagcccgg tgtcgcgggt gcactgctcc cactcgccgt tcgggccgtg
     1141 gagaccgggc gcccaggagg cgagggagac gagcaggcgc gcgccctggg cgtgcaggct
     1201 cttggcgatc cacggagagt aggcgtccgc gcacaccagg atcccgaccg gccccaccgg
     1261 ctccaccacg atcggcgcga cctgctcacc cggcgtcgac caggcctcgg agcccaccgg
     1321 cagcgtgttg accttccggt gggtgcccac gatgcgcccg tccggcgcga tgacgaagac
     1381 cgagttgtgg agcgccccgg tccgcgcgtc ccgctcggga tgagagagga agacggtcac
     1441 ccggagccgc gcgaccgtgc ggcagagatc cgccgtccag acgtcgggcg cttcgcggat
     1501 ccagtcggtg ccgatcgtgt cggcgaacgt gtagccggtg atgcacagct cgggagtgat
     1561 cacccacgcg gcgccgagct cggccgccgc ggtcaccgcg tcctccacca gccgccggtt
     1621 gtgggcgagg tcgcccgcga tcggggccag gtgcaggaag gcgagacgca ggagcatcgg
     1681 cggcgctact ctccgaggcc cagggcccgg cgcagctgct ggcgctgctc ctggaccacc
     1741 gaccacatgc ggccgccggt gagcccgccg tcgacgatga gggcgtggcc gttcacgaag
     1801 gccgagtcgt cgctggccag ccagagggcg gcccgcgcga tgtcctccgg cagccccgcg
     1861 cgctggatcg gctggcgggc gaccagcagc gtcttcatca tctccgcgat ccggtccgcc
     1921 tccggtcccg cgaggcccag cccgcctccg aagatcggcg tggcgatgcc gccggggcag
     1981 atgcagttca cgcgcacccc gctctcgccc agctccatcg ccaccgagcg ggtcaggtgc
     2041 acgacggcgg ccttggccgc gctgtagacg tgaggcccga agcccgcctg cagcccggcc
     2101 acgctcgcgg tgctgatgat gctgccgccg ccctgctgct tcatgatcgg ggcggcgtgc
     2161 ttcatgccga ggaagacgcc ccgcaggagc acgcccatcg tctgatcgaa gccctccgcc
     2221 gggatctccg cgatcggccc ggtcaccccg ccggcgcccg cgttgttgaa gacgcagtcg
     2281 aggcgtccga agcgcgcgac cgcgtgcgcg atggcgccgc tcacgtcggc ctcccggctc
     2341 acgtccacgt ggagatagct ggccgacttg ccgcccagct ccccggccag cgccgtaccc
     2401 ttgtcgtcct ggagatcggc gatcaccacg cggcagccct cctcgacgaa gagccggacc
     2461 gccgcgtggc cgatgccgct ggccccaccg gtgatgaccg caaccttgcc gtcgagcttg
     2521 cccatgggcg gtgtctccct tcagcgcgcg ccggcctgct cgagcacgcg cgccatcgtg
     2581 tcgtggccac gggcccgcgc cagggccagc ggggtccggc cgccgcggtc ggcgaggttc
     2641 acgtcggcgc cggcggtgac gagcgcggtc agggtgtcgg tgtgccgggg cccgccgtcg
     2701 cccaggacga tggactcgat gagcgcggtc caccccaggt tgttgacgtg gtcgagcggg
     2761 gcccccgccc ggatcagcgt gcgcaccacg tcggcgtggc ccaggtgagc cgccgcgatc
     2821 agcgcggtgc cgtcgtagcg gctggtgacg ctccgggagc tggcgccgag ggccagcgcg
     2881 gtctcgaggg tgcgcacgtc gccggcgacc gccgcgatgg tcacgatgtc gtagcggtca
     2941 cggtcgaggg cattgggatc ggcgcccgcg accaccagcg cgcgcatcgc ctcgtgatgg
     3001 ccgcggtggg ctgcgacgtg cagcggcgtg cggccgtggg cgtcgggccc gtccgggttg
     3061 atgccgcccg cggtcagcga ccggatgccg gcggtgtctc cgcgatcggc agcggccagg
     3121 aggccggtgt accgggcgcg ctccgccgcc gacggcggca cctgggcgcc cgcgggcgcg
     3181 gcggcggtga gggcgaccag caggccgagg agcgacgcga gcgggcggag catcggccga
     3241 cccggctggc cggccggatg tgggaggcgg agtgggagcc ggagacgcga tcatggccgg
     3301 ctcagtatac gggcggggct cgcgttccga cagcgcgccc gcctggctac ggggcccagc
     3361 cccgggtgcc gagaagcacg ccgtgattct cgcccggcgc gttcaagccg tagatccgcc
     3421 gcgccgacca gtcgaagcgc ggtgtcagct cggcgagcga ggcgggctcg acggtctcgt
     3481 ggatctggat cgcgcagagg atccgatgac cttcgaagcg tgccgcgagc acgtcgacga
     3541 tctcggcgat gggcggcgtg gtgcgccgca gatcgagccc ggtggtctgg ccgagcgcgt
     3601 cgccccacgg cggcgcgacg aagaccacca cgaggtgatc cgccggcacc gccgcgtcgg
     3661 cgaggcccgc ccggtagtcc gcattcgcca cgtcgatggg caggccgagg atcgcgaggt
     3721 tccggcgagt gaggccgaac accgcggcgt cgagctcgaa cccgagcccc cgcgctcccg
     3781 gctggtggcg cagcatccag tagagcgtgt tgccggagcc ggcgaacagg tcgatgaccc
     3841 gcgtcccttc ggaggccggc gccatgaggg cgatgccggc cacgtcccgg ccgatcgcgt
     3901 cgcccaggtc gtcccgcgtg cattcgaccg cggtgcgccc gagcaggcgg atgccccgcg
     3961 cgtaccagtc ggccggcggc aggccgtaga tcgagacgta gtcgcggtcg cggtagctgt
     4021 cggcgccgta gcgccgcacc tcctccaggt cgagcaccgc gttgcgccgg gcgccggcca
     4081 gcaggagcgc gcgatcgtag agcggcccgg tttcggtcac gcggtcagga gaaggcgagc
     4141 tgccccagct cgcacgcgag gatcgcgtca gggcaatcgg ggtgggtgcc ccgtggagtc
     4201 tggggggtga gcttcgccca ggcgcgcggc atcgacgtgc cccgcttcat gtcggcgacg
     4261 aggcgccgga agaagtgggc gaaggtttcg ccctttcggt cgagcgtcat cacgaggttg
     4321 gcgcgggcga gctgcttgtt gcggttcgcg gcgacggtgt agctctggcc cgagttgggg
     4381 gtggccacga ccaccaccgg tgcgcccgcg tcgcggatga tctcccgcag gctccgcgcc
     4441 gagttgagga tcgcgccgtc ggccgcgatc gtgcagtaga cgagcagcag ggtactgcgc
     4501 ggcggggcgt cggtgctctc gctgacctgc cggaagaggg gggccagcgc cgtccgatcg
     4561 gcggccatca gcgcgcccgc ggtgccgccc gagagatcga gcacgcccag ggtgtgggcc
     4621 tcgatccggg gcccgcgcgg agcagccagc gcaggaagcc catcgtcagg ccgagacggc
     4681 gcgcggctcc gtggtcatgg gtcgcccgcg cccaggatag cgcggagctc ggcgtcgtcg
     4741 caccaggata gcgcccacaa ccgctcgtgc ggatcgccgc gcagtccgag cgcccgggcg
     4801 aaggcgggct cggtcttcat cccgtcttct ctcaggtccc ggatggcgcg gcgcaggcga
     4861 gggctgccca ggtcgagcag ccgcgcctcc acgagcaggt gccggtaccc gcgctgcaga
     4921 tccaccggga gcgcctgccc gaacacctcg accggcagct cccgggagcg aaagcgggcc
     4981 agggtcgcga gcgtggcgcc ggccggctgc cgctcgcagg cgaagccggg ccgattggcg
     5041 aatgccccgc
//
LOCUS       DATMCV010000530         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_363484, whole genome shotgun sequence.
ACCESSION   DATMCV010000530 DATMCV010000000
VERSION     DATMCV010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363484"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VNU03_12235"
     CDS             complement(<1..927)
                     /locus_tag="VNU03_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75279.1"
                     /translation="MDEHRLGELIACVKDGRLTRRSFVQAMAAVGLGAPMAGSLLGAA
                     GAATAQPREAEFTPTRRGGGGTLRMLMWDAPTLLHPHFGRGLRDFTVQRIFYEPLAAA
                     APDGTLVPVLAEDIPNRKANTLAKDGQWVTWRLKRNVVWHDGAPFTADDVIFNWEFAL
                     DPATAAGTRASYEDITRIERLDQYTIKVIFKKPQPYWASAFTGGGLLPRHIFQTVKGA
                     GAREAIGMIPAVGTGPYRLTEFRPGDLIRAELHKGYHVPNRPFFDRLDIKCGGDAVGA
                     ARAVLQTGEYDFAYYVLIEEEVLRRIEQGGKGR"
     gene            complement(1021..>2091)
                     /locus_tag="VNU03_12240"
     CDS             complement(1021..>2091)
                     /locus_tag="VNU03_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75280.1"
                     /translation="SVSKRFVRAEQGAAAVADVSLTVEPGELLTLLGPSGCGKTTTLR
                     MVAGFERPDTGQVLIDEQDVTGLMVYRRSIGFVFQNYALFPHLTVFENVAYGLRVQRL
                     REPVVRERVGRVLALVGLPGYERRFPNQLSGGEQQRVAVARAVVVEPRLLLFDEPLSN
                     LDAKLRVQMREELSRLQRQLGITTVYVTHDQEEAMAISDRIAVMQAGRVAQVGTAHDL
                     YRAPRSVFVAQFIGRVNLLPARVVRVSPGLATVALWGREVTVPADGAGGGGPDVSLVL
                     RPESVGLRPEGASAPDGEITVRGVVRGRTFLGEKVEYAVEVDGASLLTVSYDPLRHGL
                     FESGARVQVSCAPASLRLLGAA"
BASE COUNT          297 a          762 c          759 g          273 t
ORIGIN      
        1 ccggcccttg ccgccctgct cgatgcgccg gagcacctcc tcctcgatca gcacgtagta
       61 ggcgaagtcg tactcgccgg tctgcagcac cgcgcgcgcg gcgcccaccg cgtcgccgcc
      121 gcacttgatg tcgaggcggt cgaagaatgg gcgattcggg acgtgatagc ccttgtggag
      181 ctcggcgcgg atcaggtcac cgggccggaa ctcggtgagc cggtagggcc cggtgccgac
      241 cgcggggatc atgccgatcg cctcgcgcgc gccggcgccc ttgacggtct ggaagatgtg
      301 gcgcggcagc agacctccgc cggtgaacgc ggacgcccag tagggctgcg gcttcttgaa
      361 gatgaccttg atcgtgtact ggtcgagccg ctcgatccgg gtgatgtcct cgtaggaggc
      421 gcgggtcccc gccgcggtgg ccggatcgag ggcgaactcc cagttgaaga tgacgtcgtc
      481 cgcggtgaac ggggcgccgt cgtgccacac cacgttgcgc ttgagccgcc aggtcaccca
      541 ctgaccgtcc ttggcgagcg tgttggcctt gcggttcggg atgtcctcgg ccagcaccgg
      601 caccagggtg ccgtcgggcg ccgcggcggc gagcggctcg tagaagatgc gctggaccgt
      661 gaagtcgcgc aggccccggc cgaagtgcgg gtgcagcagg gtgggggcgt cccacatcag
      721 catccggagg gtgccgccgc ccccgcgccg ggtcggggtg aactcggcct cgcggggctg
      781 ggcggtcgcg gctcccgccg cgcccaggag ggaccccgcc atcggggcgc cgagcccgac
      841 cgcggccatc gcctgcacga agctccgccg ggtgaggcgg ccgtccttca cacacgcgat
      901 cagctcgccc agcctgtgct cgtccatgtg cctccgaggg gtccgcgtgg gtagagggtc
      961 cgacagcgat aacgcgggcg cgccccgaag tcaagggcgc ccgcgtccgg agccgcgagc
     1021 ctaggccgct ccgagcagcc ggagcgacgc gggcgcgcag ctgacctgca ccctcgcgcc
     1081 gctctcgaag aggccgtggc ggagggggtc gtaggagacg gtcaggagcg acgccccgtc
     1141 cacctcgacc gcgtattcga ccttctcgcc caggaacgtg cggccgcgga ccacgccgcg
     1201 gaccgtgatc tccccgtccg gggcggaggc cccttcgggc cgcaggccga ccgattccgg
     1261 ccgcagcacg agggagacgt ccggaccgcc cccgccggcg ccgtcggccg gcaccgtgac
     1321 ctcgcgcccc cacagggcca ccgtggcgag acccgggctc acgcgcacca cgcgcgcggg
     1381 caggaggttc acgcggccga tgaactgggc cacgaagacc gagcggggcg cgcggtagag
     1441 atcgtgggcg gtgcccacct gggccacccg tcccgcctgc atcaccgcga tacggtccga
     1501 gatggccatg gcctcctcct gatcgtgggt cacgtagacg gtggtgatgc cgagctggcg
     1561 ctgcagccgc gacagctcct cccgcatctg cacccgcagc ttggcgtcca ggttgctgag
     1621 cggctcgtcg aagaggagca gccggggctc gaccaccacc gcgcgcgcca ccgccacccg
     1681 ctgctgctcg ccgcccgaga gctgattcgg gaagcgacgc tcgtagccgg gcaggccgac
     1741 cagggccagc acgcgcccga cccgctcgcg cacgaccggc tcccgcagcc gctgcacccg
     1801 gaggccgtag gccacgttct cgaagacggt gaggtgcggg aagagcgcgt agttctggaa
     1861 cacgaagccg atgctccgcc ggtacaccat gaggccggtc acgtcctgct cgtcgatcag
     1921 cacctgcccg gtgtcgggcc gctcgaagcc cgccaccatc cgcagcgtgg tggtcttgcc
     1981 gcagccggag ggaccgagca gggtcagcag ctcgcccggc tccaccgtga ggctcacgtc
     2041 ggccaccgcg gccgcgccct gctcggcccg gacgaagcgc ttggagacgg a
//
LOCUS       DATMCV010000531         2263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_363488, whole genome shotgun sequence.
ACCESSION   DATMCV010000531 DATMCV010000000
VERSION     DATMCV010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363488"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VNU03_12245"
     CDS             <1..1121
                     /locus_tag="VNU03_12245"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWP75281.1"
                     /translation="YVEVLDRAFETYTRWIDPMREYWMRRFPQPAGESDAAYRMTIRA
                     KGLDSLRGLLPAATRSNVGIFGTGQGYEALLLRMRAHPLREVRDYGALMLVELQKVIP
                     AFLLRLERPERGGVWARYLEETREATAAVASRLLEDVEPESRPEVTLTDFDPDGEVKV
                     VAAALYAVSALPDDQLLAVARKLGDEARREVLDAYVGRRENRRHKPGRAFERTVYRFD
                     VLGDYGAFRDLQRHRLLTLEWQPLSTRHGYVTPDELGEAGVLPEWTRVMEDAAALHDA
                     LAAAAPAAAAYAVPMAYRVRFYMELNAREAMHMIELRTAPQGHPAYRRICQAMHRLIA
                     EQAGHRALAGAMRHADHSGAEGGRLEAERAAERRRLGR"
     gene            1321..1638
                     /locus_tag="VNU03_12250"
     CDS             1321..1638
                     /locus_tag="VNU03_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75282.1"
                     /translation="MKTTRPLLVGLLPLILTAGCATQSAYLNEGPTALGRPPYQVGPV
                     TVVMRPQAEVELICRLRSPHAAPEGRIHGCYVPADRLIVSTADPYVLMHEFKHYFEGP
                     WHK"
     gene            1769..>2263
                     /locus_tag="VNU03_12255"
     CDS             1769..>2263
                     /locus_tag="VNU03_12255"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWP75283.1"
                     /translation="MGPLRRDQIVRATIRCLARDGYAGLTMKRVAAEAGVSQGILHYY
                     FRDKAAMLAAAALRVTADLDRRVAVQARGARTARARLRAVIRACLETAVESRDFWTVF
                     IEFWGEALHDRRLAAVNRQAYARARRLIGAALARGRAQGGVRRVDLEEAAAVVLGLLD
                     GLSLQ"
BASE COUNT          314 a          815 c          808 g          326 t
ORIGIN      
        1 gctacgtgga ggtcctggat cgcgcgttcg agacctacac ccgctggatc gatccgatgc
       61 gcgagtactg gatgcggcgc ttcccgcagc cggcggggga gagcgacgcg gcctaccgca
      121 tgacgatccg ggcgaagggg ctcgacagtc tccgcgggct cttgcccgcg gcgacccggt
      181 cgaacgtggg catcttcggg accggccagg gctacgaggc gctcctgctc cgcatgcggg
      241 cccatccgct ccgggaggtc cgcgactacg gggcgttgat gctggtcgag ctgcagaagg
      301 tgattcccgc gttccttctg cgcctcgagc ggccggagcg cggcggcgtc tgggcgcggt
      361 acctggagga gacgcgcgag gccaccgccg cggtggcctc gcgcctgctc gaggacgtcg
      421 agcccgagtc ccggcccgag gtcaccctca ccgacttcga tccggacggg gaggtgaagg
      481 tggtggccgc cgcactctac gcggtctccg cgcttccgga cgaccagctc ctggccgtcg
      541 cgcgcaagct cggcgacgag gcgcgccgcg aggtgctcga cgcctacgtc ggccgtcgcg
      601 agaaccgccg ccacaagccg ggccgggcgt tcgagcgcac cgtctatcgc ttcgacgtgc
      661 tcggcgacta cggcgcgttc cgggacctgc agcgtcaccg gctgctgacg ctcgagtggc
      721 aaccgctctc gacccggcac ggctacgtga cgcccgacga gctcggggaa gcgggggtgc
      781 tcccggagtg gacccgcgtg atggaggacg cggccgcgct ccacgacgcc ctcgcggccg
      841 ccgcgcccgc cgcggccgcc tacgcagtgc cgatggccta ccgggtgcgg ttctacatgg
      901 agctgaacgc gcgcgaggcc atgcacatga tcgagctgcg caccgcgccg caggggcacc
      961 ccgcgtatcg ccggatctgc caggccatgc accgcctgat cgccgagcag gccgggcacc
     1021 gagcgctcgc cggggccatg cggcacgccg accactcggg cgcggaaggc ggacgactcg
     1081 aagcggagcg ggccgcggag cgtcggcgtc tcggacgctg agccgggggc agcgcgtcgg
     1141 ccaaggtccg gccgctatgg cgccgtctcc atgactctgg gctcgccacg acagaatgcc
     1201 ctcggccgcg cccgctcaca ctgagccagc cgcagattgg taagtagttg atgtggcggg
     1261 tgtttatgcc cgtctcgatc gtcccccacc cggtggcatc gaggttgccc acaagagggt
     1321 atgaaaacga cacgccccct cctcgtgggc ctcctgccgc tcatcctcac cgcggggtgc
     1381 gcgacgcaga gcgcctatct gaacgaaggg cccaccgcgc tcggcaggcc cccgtaccag
     1441 gtggggccgg tgacggtggt catgcgcccc caggccgagg tggagttgat ctgccgcctg
     1501 cggagcccac acgcggcgcc ggaaggccgg atccacggct gctacgtgcc ggccgatcgc
     1561 ctgatcgtct cgaccgccga cccctacgtc ctgatgcacg agttcaagca ttacttcgag
     1621 ggaccctggc acaagtaggc tcgcggtagt ctcccccgta gtccccgctt gacccgcccg
     1681 aacgttcgcg gcatacttcc tcgtccgaga taattggtcg accaatcaat tataaatcta
     1741 gagctcgaat ctgactccca agctcgggat ggggccgctg cggcgcgacc agatcgtgcg
     1801 ggccaccatc cgctgtctcg cccgcgacgg ttacgcgggc ctcaccatga agcgggtcgc
     1861 cgcggaggcc ggggtgagcc agggcatcct ccactactac ttccgcgaca aggcggcgat
     1921 gctcgccgcc gccgccctgc gggtgaccgc cgatctcgac cgccgggtgg cggtccaggc
     1981 gcgcggcgcc cgcaccgccc gggcgcggct gcgcgcggtg atccgcgcct gcctcgagac
     2041 cgcggtggag agccgcgact tctggaccgt gttcatcgag ttctggggcg aggcgctcca
     2101 cgatcggcgc ttggccgcgg tcaaccgcca ggcctacgcg cgggcgcggc ggttgatcgg
     2161 cgccgcgctg gcgcgcgggc gggcccaggg tggggtgcgc cgggtcgacc tcgaggaggc
     2221 cgccgcggtg gtcctggggc tcctcgacgg cctctccctg cag
//
LOCUS       DATMCV010000532         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_163739, whole genome shotgun sequence.
ACCESSION   DATMCV010000532 DATMCV010000000
VERSION     DATMCV010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163739"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VNU03_12260"
     CDS             complement(<1..468)
                     /locus_tag="VNU03_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75284.1"
                     /translation="MTVKYRIGIMPGPWPPGRDGAAFLWTLCDQLERSDVDSIWLSDR
                     LSSPAPVPEVMTTLAAIAARTTRLKFGPSVVVLPYRTPVVAAKEMATVDWLSQGRLFP
                     AVGVGVELPREFDASGVPFAERGRRTDEAIRVMRLLWTQDEVTFQGDFYKLDRV"
     gene            564..1205
                     /locus_tag="VNU03_12265"
     CDS             564..1205
                     /locus_tag="VNU03_12265"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWP75285.1"
                     /translation="MSGHDRRSFPRAAASWPATVELADGRVVSGEVVNLSLSGMRVRS
                     EIDVAVNSVVTVRVTLPGEAGRLEMVGTVVRRDRESIGVAFLKMSDSPAGRVTPFVSR
                     SDTRRRYPRVPVSLPIQVEGGSAGTARGHTVDLSASGGRVTTDQPLVDGDVVVIELPA
                     RPGLDRLRLPALVWEAYEGGAVLVFTNLDSRDFTRLQEYIASCQSRGSRPTSV"
     gene            1253..1837
                     /locus_tag="VNU03_12270"
     CDS             1253..1837
                     /locus_tag="VNU03_12270"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWP75286.1"
                     /translation="MRDPDKPQQIIEAAVRVFARQGYFNSRVSDIAREAGIAAGTIYL
                     YFKTKEDILITLFREKMAAFEASLRRAIAGEPDAVAKVRRLVQLHFQMLEQDPDLAEV
                     VQVELRQGQKFFRGASSQEVAAYFALIGSVLEEGVGQGHFKADLPVKVATKVLFGAMD
                     QMATSWVLGKRGYRLVDTAPAVADLFLQGVAARP"
     gene            1863..2636
                     /locus_tag="VNU03_12275"
     CDS             1863..2636
                     /locus_tag="VNU03_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP75287.1"
                     /translation="MAYETLILTREERLAIVTLNRPPANAISETLMRELNAALSELES
                     DDAVRAVIITGAGDKIFCAGADLGSAFQGSDVGTFIRFGNSVVRRIERFPKPVVAAIN
                     GHALGGGCEIAMGCHIRILKQGARMGQTESNLGIIPGFGGTQRYPRLMGRGRALEHLI
                     LGTQVPAEECYRTGLVNRLSKEGETLNDARALARELAKRPPIATRLIIEAVDDGIHAP
                     IDQAIEVEVRAFLQSLRTEDAGEGIQAFFQKRPADFKGR"
     gene            2682..>2804
                     /locus_tag="VNU03_12280"
     CDS             2682..>2804
                     /locus_tag="VNU03_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75288.1"
                     /translation="MTGGARSLGKQDALTLASEGCRVIVLDLNAEGAAETAKEIA"
BASE COUNT          424 a          962 c         1015 g          403 t
ORIGIN      
        1 cacccggtcc agcttgtaga agtcgccctg gaaggtcacc tcgtcctgcg tccagagcag
       61 gcgcatcacc cggatggcct cgtcggtgcg gcgcccgcgt tccgcgaagg gcacgccgga
      121 ggcgtcgaac tcgcgcggca gctcgacccc cacccccacc gcggggaaga gccgcccctg
      181 cgagagccag tccaccgtcg ccatctcctt ggccgccacc accggggtgc gatacgggag
      241 caccaccacg ctcggcccga acttgagccg ggtggtgcgc gccgcgatcg cggcgagcgt
      301 ggtcatgacc tccgggaccg gagcggggga ggagagccga tcggagagcc agatcgagtc
      361 cacgtcgctc cgctcgagct ggtcgcagag ggtccacagg aaggccgcgc cgtcgcggcc
      421 gggtggccac gggcccggca tgatgccgat gcggtacttg acggtcatgg tgcggcatcg
      481 tacgcgcggc ggtcggattg tcaaggcgag cggcgcgccc gcaagagttg acaggaaggc
      541 cccggacacg tcagggtcga gaggtgagcg gtcacgatcg gcggtcgttt cccagagcgg
      601 cggcgtcctg gccggccacg gtggagctgg ccgatggccg cgtggtgtcc ggcgaggtcg
      661 tcaacctgag cctctccggg atgcgggttc gatcggagat cgacgtggcg gtgaacagcg
      721 tcgtcacggt gcgcgtgacc ctgcccggcg aggcgggccg gctcgagatg gtgggcacgg
      781 tggtgcgccg cgaccgggag agcatcgggg tcgcattcct caagatgtcc gactcgcccg
      841 cgggccgggt gaccccgttc gtctcgcgca gcgacacccg ccggcgctat ccccgggtgc
      901 cggtgagcct gccgatccag gtggagggcg ggtccgcggg caccgcgcgc ggtcacaccg
      961 tggacctgag cgcgtcgggc ggccgggtga ccaccgatca gccgctggtc gacggcgacg
     1021 tggtcgtgat cgagctgccc gcgcggccgg ggctcgaccg cctgcggctg cccgcgctgg
     1081 tgtgggaggc gtacgagggc ggcgcggtgc tggtgttcac caacctcgat tcccgggatt
     1141 tcacgcggct gcaggagtac atcgcgagct gccagtcgcg cggctcgcga ccgacttccg
     1201 tttgacagcc ccgggagcgc cgaatagaat gaaccgcggt tcatctcttc ccatgcgcga
     1261 tccagacaag ccccagcaga tcatcgaggc cgcggttcgg gtcttcgcgc ggcagggcta
     1321 cttcaactcg cgggtgtcgg acatcgcgcg cgaggcgggc atcgccgccg gcacgatcta
     1381 cctctatttc aagaccaagg aagacatcct catcacgctc ttccgcgaga agatggccgc
     1441 gttcgaggcg tctctgcggc gggccatcgc cggtgagccg gacgcggtcg ccaaggtgcg
     1501 ccggctcgtc cagctgcact tccagatgct cgagcaggat cccgacctcg ccgaggtcgt
     1561 gcaggtcgag ctgcgccagg ggcagaagtt cttccggggc gcgtcgagcc aggaggtcgc
     1621 ggcgtacttc gccctgatcg gctccgtcct cgaggagggc gtcggccagg ggcacttcaa
     1681 ggccgacctg ccggtgaagg tggccaccaa ggtgctcttc ggcgcgatgg accagatggc
     1741 cacgtcgtgg gtcctgggca agcgcggcta ccggctcgtg gataccgcgc cggcggtggc
     1801 ggacctgttc ctgcagggcg tggccgcccg cccctagcct tcacgcacgg aggagtcacc
     1861 cgatggccta cgagaccctc atcctgaccc gcgaggagcg cctcgcgatc gttaccctca
     1921 accggccgcc cgcgaacgcc atcagcgaga cgctcatgcg ggagctgaac gcggcgctct
     1981 ccgagctcga gtcggacgac gcggtgcgcg cggtgatcat caccggcgcg ggcgacaaga
     2041 tcttctgcgc gggcgccgat ctcggctccg ccttccaggg atcggacgtc ggcaccttca
     2101 tccgcttcgg caacagcgtg gtgaggcgca tcgagcgctt tcccaagccg gtggtggccg
     2161 cgatcaatgg ccacgcgctg ggcggcggct gcgagatcgc gatgggctgc cacatccgca
     2221 tcctgaagca gggggcgcgg atggggcaga ccgagtccaa cctcggcatc atcccgggct
     2281 tcggcggcac ccagcgctac ccgcgcctca tggggcgcgg gcgcgccctc gagcacctga
     2341 tcctcgggac ccaggtgccc gcggaggagt gctaccgcac cgggctcgtc aaccggctgt
     2401 cgaaggaggg cgagacgctc aacgacgctc gtgcgctcgc ccgcgagctg gccaagcgcc
     2461 cgccgatcgc cacgcgcctc atcatcgagg cggtggacga cggcatccac gcccccatcg
     2521 accaggccat cgaggtcgag gtgcgagcct tcctccagtc gctccgcacc gaggacgcgg
     2581 gcgagggcat ccaggccttc ttccagaagc ggcccgccga tttcaagggc cgctaggtgg
     2641 attcctaagt ggacctgggc cttacgggca agaccgcgct ggtgaccggc ggcgcccgga
     2701 gcctcggcaa gcaggacgcg ctcaccctgg catcggaagg ctgccgggtg atcgtgctgg
     2761 atctcaacgc ggagggcgcg gccgagaccg cgaaggagat cgcg
//
LOCUS       DATMCV010000533         3674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_363489, whole genome shotgun sequence.
ACCESSION   DATMCV010000533 DATMCV010000000
VERSION     DATMCV010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363489"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="VNU03_12285"
     CDS             <1..1508
                     /locus_tag="VNU03_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP75289.1"
                     /translation="WLGENFFPTSDNGQFILHLRAKTGTRIEETARLADLVEASIRRE
                     IPPEELDNIIDNIGLPYSTINYMYSRSGFIGTSDADILVTLKEGHRPTPDHMRTLRAR
                     LPQEFPGVSFYFVPADIVTQILNFGLPAPIDVQVEGNDIEGNRQVANKILADLRQVSG
                     LTDLRIQQTFDYPNFQVSVDRTKAAGAGFTPRDVANSVLLTLSGSGQTTPIQFLNWQN
                     GVSYNLIAQAPQYSVQSLQDLQNVPISGANPARPQILGDMASISRTTGMAVISHYNTR
                     RVVDIYGSVQDRDLGAVGRDITRIVDAHRPNLPRGSFVTLRGQVETMRASYVGLLGGL
                     AFAIVLVYLLIVVNFQSWLDPFIIITALPAALAGIIVFLFLTGTTLSVPALMGAIMCM
                     GVGTANSILVVSFAKERLGEHGDAREAAIEAGVTRFRPVLMTALAMVIGMIPMALGLG
                     EGGEQNAPLGRAVIGGLVCATIATLVFVPAVFSLLHGRRRPVAPAAPEPAA"
     gene            1505..2686
                     /locus_tag="VNU03_12290"
     CDS             1505..2686
                     /locus_tag="VNU03_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWP75290.1"
                     /translation="MRGSRGVWILLLVVLALAVAIYSGLHGRRGADAALKRATQEAAV
                     PTVAVIYPSASSPSDELVLPGTARAFTDAPIYARANGYLKKWYADIGTRVTQGQILAE
                     IETPELDQQLRQARADLENARANMDMARTTAERWTTLLKRDAVSRQETDEKVSDYNGK
                     RAIVESNGANVQRLENLQAFQRITAPFDGVITARNTDVGALIDSGASAQARELFRLAS
                     TSKLRVFVSVPQAYAQAARPGTPTQITLEENPGKIYRGTLARTSTALDPTARTMLSEV
                     EMDNPNAEVLPGAYVVVRLRVGLETKGMTIPANTLLFRSEGLRVAVVRDGRAELVPVT
                     LGRDYGRSVEVVAGLQARDAVILDPADSLVSGAPVRVRPTEPPAGKPGEPPPAAKPPS
                     G"
     gene            complement(2595..3521)
                     /locus_tag="VNU03_12295"
     CDS             complement(2595..3521)
                     /locus_tag="VNU03_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HWP75291.1"
                     /translation="MRYRWPDGKQSAAVLSFDFDAESGFLFREPEKARRSLGDLEERR
                     FGPRVGVDRILRLLDRLKLRASFYIPGWTVENHPAESARIRDAGHEIGAHGNMHEALS
                     FLDEAQEERVMREQLDILQSRLGVRPAGYRSPSWDVNVWTPALLKRHGFLYDTSLMGN
                     DVPYEVETGQGPLIEVPVQWLLDDAPLFRHVYGATNAIADPGRVLQMWSKEFAAMHRE
                     NGCFVLTCHPFVSGRASRIALLEDLVTYMRRSRGVWFATGEEVARWHASRLAAAPAAR
                     AAATRLAASRPAAVRPASRPAARSAARGRGRR"
     gene            complement(3547..>3674)
                     /locus_tag="VNU03_12300"
     CDS             complement(3547..>3674)
                     /locus_tag="VNU03_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75292.1"
                     /translation="FLRVAPHVSVFPGLTIMLTVLGLNLLGDGLRDVLDPRAGPA"
BASE COUNT          571 a         1334 c         1271 g          498 t
ORIGIN      
        1 cgtggctcgg cgagaacttc ttcccgacca gcgacaacgg gcagttcatc ctgcacctgc
       61 gggccaagac cggcacccgc atcgaggaga ccgcccggct ggccgacctg gtggaggcgt
      121 cgatccggcg agagatcccc cccgaagagc tcgacaacat catcgacaac atcgggctgc
      181 cgtactcgac gatcaactac atgtacagcc gctccggctt catcggcact tcggacgccg
      241 acatcctggt gactctcaag gaaggccacc ggcccacccc ggaccacatg cgcaccctgc
      301 gggcgcggct gccccaggag ttcccggggg tgagcttcta cttcgtgccc gccgacatcg
      361 tgacccagat cctcaacttc ggcctgcccg ccccgatcga cgtgcaggtc gagggcaacg
      421 acatcgaggg caaccgccag gtggcgaaca agatcctcgc cgacctgcgg caggtgtccg
      481 ggctcaccga cctgcgcatc cagcagacct tcgactaccc gaacttccag gtgagcgtcg
      541 accgaacgaa ggccgcgggc gcggggttca cccctcgaga cgtcgccaac agcgtgctgc
      601 tcaccctgag cgggagcggc cagaccactc cgatccagtt cctcaactgg cagaacgggg
      661 tcagctacaa cctgatcgcc caggcgccgc agtactcggt ccagtcgctc caggatctcc
      721 agaacgtgcc gatcagcggg gcgaaccccg cgcgcccgca gatcctcggc gacatggcct
      781 cgatctcgcg caccaccggg atggcggtga tctcgcacta caacacccgg cgggtggtcg
      841 acatctacgg ctcggtgcag gaccgggacc tcggcgcggt cggccgcgac atcacccgga
      901 tcgtcgacgc gcaccgcccg aacctgccgc ggggaagctt cgtgaccctg cgcgggcagg
      961 tggagaccat gcgggcctcc tacgtgggcc tgctcggcgg gctcgcgttc gcgatcgtcc
     1021 tcgtctatct tctgatcgtc gtgaacttcc agtcctggct cgatcccttc atcatcatca
     1081 ccgcgctccc cgccgccctc gccggcatca tcgtgttcct gttcctgacc ggcaccaccc
     1141 tgagcgtgcc ggccctgatg ggcgcgatca tgtgcatggg ggtcggcacc gcgaacagca
     1201 tcctggtggt gtccttcgcc aaggagcggc tgggcgagca cggcgacgcg cgcgaggccg
     1261 ccatcgaggc gggcgtcacc cgcttccggc cggtgctcat gaccgcgctc gccatggtga
     1321 tcggcatgat cccgatggca ctgggcctgg gcgagggcgg cgagcagaac gcgccgctcg
     1381 gccgggcggt gatcggcggg ctcgtgtgcg ccaccatcgc caccctggtc ttcgtccccg
     1441 cggtgttcag cctgctgcac gggcggcgcc gtcctgtcgc tcccgccgcg ccggagccgg
     1501 ccgcttgagg ggctcgcgcg gggtctggat cctcctgctc gtcgtcctcg cgctggcggt
     1561 cgccatctac tccggcctcc acgggcggcg gggagccgac gcggcgctga agcgggcgac
     1621 ccaggaggcg gcggtcccca cggtggcggt gatctacccg tccgcctcct cgccgagcga
     1681 cgagctggtg ctgccgggca ccgcgcgcgc cttcaccgac gcgccgatct acgcgcgcgc
     1741 caacggctac ctcaagaagt ggtacgccga catcgggacc cgggtaactc agggtcagat
     1801 cctggccgag atcgagacgc ccgagctcga ccagcagctc cggcaggcgc gcgcggatct
     1861 ggagaacgcg cgagccaaca tggacatggc gcgcaccacc gcggagcggt ggacgaccct
     1921 gctcaagcgc gacgcggtct cgcgccagga gaccgacgag aaggtcagcg actacaacgg
     1981 caagcgggcc atcgtcgagt cgaacggcgc gaacgtgcag cggctcgaga acctccaggc
     2041 cttccagcgc atcaccgcgc ccttcgacgg ggtgatcacc gcgcggaaca ccgacgtggg
     2101 cgcgctgatc gactcggggg cgagcgccca ggcccgcgag ctgttccgcc tcgcctcgac
     2161 cagcaagctg cgcgtcttcg tctcggtgcc gcaggcctac gcgcaggccg cacgccccgg
     2221 caccccgacc cagatcaccc tcgaggagaa cccgggcaag atctaccggg gcacgctggc
     2281 ccgcacgtcg accgcgctcg acccgaccgc ccgcaccatg ctgagcgagg tcgagatgga
     2341 caacccgaac gccgaggtcc tgcccggcgc gtacgtggtg gtccgcctgc gggtcgggct
     2401 cgagacgaag gggatgacca tcccggccaa caccctgctc ttccgctcgg aggggctgcg
     2461 ggtcgcggtg gtgcgggacg ggcgcgccga gctggtgccg gtgaccctcg ggcgcgacta
     2521 cggccggagc gtcgaggtgg tagccggcct ccaggcacgc gacgcggtga tcctcgatcc
     2581 cgccgactcg ctcgtcagcg gcgcccccgt ccgcgtgcgg ccgaccgagc cgccggccgg
     2641 gaagccgggc gaaccgccgc cggccgcgaa gccgccaagc gggtagccgc cgcccgcgcg
     2701 gcgggagcgg ccgcgagccg gctcgcgtgc cagcgcgcca cctcctcgcc ggtggcgaac
     2761 cacaccccgc gggagcggcg catgtaggtc accaggtcct ccagcagcgc gatgcgggaa
     2821 gcccgcccgc tcacgaacgg gtggcaggtc agcacgaagc agccgttctc ccggtgcatc
     2881 gcggcgaact ccttgctcca catctgcagc acgcggcccg gatccgcgat cgcgttggtg
     2941 gcgccgtaca cgtgacggaa gaggggcgcg tcgtcgagca gccactgcac cgggacctcg
     3001 atcagcggcc cctgaccggt ctcgacctcg tagggcacgt cgttgcccat cagcgaggtg
     3061 tcgtagagga agccgtggcg cttcaggagg gcgggggtcc acacgttgac gtcccacgag
     3121 ggcgagcggt agccggccgg gcgcacgccc aggcgcgatt gcagaatatc gagctgctcg
     3181 cgcatcaccc gctcctcctg ggcctcgtcg aggaagctca gcgcctcgtg catgttaccg
     3241 tgcgcgccga tctcgtggcc cgcgtcgcgg atgcgggcgg actcggccgg atggttctcc
     3301 acggtccagc ccgggatgta gaagctggcc cggagcttca gccgatcgag caggcgcagg
     3361 atgcggtcga ccccgacgcg ggggccgaag cgccgctcct cgagatcgcc gaggctgcgg
     3421 cgcgccttct cgggctcgcg gaagaggaag ccggactcgg cgtcgaagtc gaaggacagg
     3481 actgcggcgg actgcttgcc gtcgggccag cggtagcgca tcgcgatctc ccggggagcg
     3541 tggtggctag gcggggcccg cgcgcgggtc gagcacgtcg cgcaggccgt cgccgagcag
     3601 gttgaggccg agcaccgtca gcatgatggt gaggccgggg aacaccgaga cgtggggagc
     3661 cacccgcagg aagt
//
LOCUS       DATMCV010000534         4870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_560677, whole genome shotgun sequence.
ACCESSION   DATMCV010000534 DATMCV010000000
VERSION     DATMCV010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560677"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VNU03_12305"
     CDS             <1..843
                     /locus_tag="VNU03_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP75293.1"
                     /translation="HSFGLNGALLAPLLAGVTVVLQERFAPEETLRAIGRHRVTVFPG
                     VATMFARILESPALDLADLASLRLAVSGAAPCPWALASEWRRRTGVRIARGYGMTELF
                     RPISYLAAETTDRPDSIGRAVPGVELRVEDDELWIRSPAAMDGYLRAPEETGAVLGTD
                     GWFRTGDLATISDDGYVSIVGRKRELILRGGYSVVPGEVEAALLGHPAVAEAAVIGVA
                     HPELGEEVAAFVTLRPGTSAEAPELIAFCRERLAGYKYPRRVTVVDAMPRSATGKILK
                     AKLR"
     gene            complement(923..1192)
                     /locus_tag="VNU03_12310"
     CDS             complement(923..1192)
                     /locus_tag="VNU03_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75294.1"
                     /translation="MKTNHVMSLVAATVFALGVAGPALAQTPAPAPSTTPAPAAPSKG
                     DSQMDKMDKKDGKAKHTAKKKAAKKSEGDSAAKAPDTKTPEQPKK"
     gene            complement(1232..1555)
                     /locus_tag="VNU03_12315"
     CDS             complement(1232..1555)
                     /locus_tag="VNU03_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75295.1"
                     /translation="MKSRLTSFVALLALIVAAAPAGAADDTKAREGAAQVESGAKQVG
                     RGVEDTAKGIGKTVVGGAETAGERVKEAGRAAEPEAKSAWSRTKEGAVSFGHSVKSFF
                     TNLFQ"
     gene            complement(1751..2146)
                     /locus_tag="VNU03_12320"
     CDS             complement(1751..2146)
                     /locus_tag="VNU03_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWP75296.1"
                     /translation="MRLTKAQAKMLERERVARVATVGDGMPHLVPVCHVLAGGKIYFG
                     SGRKGRKVLNVRANPRLALTVDLYSDDWAHITGVMVQGRARVIERGPRFRGLRRRLYA
                     KYPQYPREAALGETDSVIVELTPTHVFSW"
     gene            2224..2427
                     /locus_tag="VNU03_12325"
     CDS             2224..2427
                     /locus_tag="VNU03_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75297.1"
                     /translation="MKSKASTKKSKTSSARAKAKPQPKPAARKAAAKAPAARATVAAA
                     PKAQAYTPKPIEGIGWAPFRYSR"
     gene            2475..3224
                     /locus_tag="VNU03_12330"
     CDS             2475..3224
                     /locus_tag="VNU03_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HWP75298.1"
                     /translation="MAVLGALLLLPLAAGCAPLERLGQNWWELRRDSSGQAPDPASTP
                     DAVIQVYAARTVGWRGVMAVHTWLTLKPAGAPAYTRYEVMGWGVGNGAPAIRVNRSGP
                     DNYWFGSRPELLVDRRGEGVDGLITRVQAAVESYPYAASYRTWPGPNSNTFTAYLGRA
                     VPELRLDLPATAIGKDFIPGGLPAAMAPSGTGVQLSLFGLLGVLAGAEEGLELNVLSL
                     TFGVDVKRPALKLPVVGRLGFSRRPPPPAPE"
     gene            complement(3190..4293)
                     /locus_tag="VNU03_12335"
     CDS             complement(3190..4293)
                     /locus_tag="VNU03_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWP75299.1"
                     /translation="MTTPSAPRDAAEVSSTADTIPVRETHRFDVAALERYMAGHVGGF
                     KGPVTVTQFQGGQSNPTYRLSSPGGEYVLRRKPPGKLLPSAHAVDREYRIITALASTR
                     VPVPRTYALCEDDAVIGTAFFIMEWVRGRVVADPLLPGMSPPDRACIYGSMNEVLATL
                     HRVDLEATGLADYGRPGSYFARQIHRWTTQYRASETERIEAMEQLIAWLPAHVPADER
                     PTVVHGDFRPGNLILHPTEPRVVAVLDWELSTLGSPLADLAYNCMPYRLGPSTLGGVL
                     GAPLAEMGLPSEAEYVATYCRRTDRSGIPDWEFYLAFAMFRLAAIAQGIMGRVIAGTA
                     NDVNARARGERARPLAEAGWETITRGPGVAAAG"
     gene            complement(4375..>4870)
                     /locus_tag="VNU03_12340"
     CDS             complement(4375..>4870)
                     /locus_tag="VNU03_12340"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75300.1"
                     /translation="AARIAALFNMSNPALPPQWREIERATRSLEIEPILLDARKVEDL
                     EPAFRSAIRQRADALIVGLDTLNQANQGVIVDLAAKYRLPAIYASTEFAHGLVAYGVN
                     YPEMYRRAAGLAHKIFKGARPADLPVEEPTAFEFVINLKTARTLGLKIPPSLLLRADH
                     VIEY"
BASE COUNT          645 a         1693 c         1780 g          752 t
ORIGIN      
        1 cactccttcg ggctcaacgg cgcgctgctg gccccgctgc tcgccggcgt cacggtggtg
       61 ctacaggagc gcttcgcgcc cgaggagacg ctgcgcgcga tcggccggca ccgggtgacc
      121 gtgttccccg gtgtggccac catgttcgcg cgcattctcg agtcgcccgc gctcgacttg
      181 gccgatctgg cgagcctgcg cctcgcggtc tcgggcgccg ccccgtgccc gtgggcgctg
      241 gccagtgagt ggcggcggcg caccggcgtg cgcatcgcac gcggctacgg gatgaccgag
      301 ctgttccggc ccatctccta tctcgccgcg gagacgaccg accggcccga ctcgatcggc
      361 cgcgcggtgc cgggcgtcga gctgcgggtg gaggacgacg agctgtggat ccgctcgccc
      421 gccgcgatgg acggctacct gcgcgcgccc gaggagaccg gggcggtgct aggcaccgat
      481 gggtggttcc ggaccggcga cctggccacc atctccgacg acggctacgt cagcatcgtg
      541 gggcgcaagc gcgagctgat cctgcgcggc ggctactcgg tggtgccggg cgaggtggag
      601 gccgcgctgc tcggccaccc ggcggtggcg gaggccgcgg tgatcggggt ggcgcacccg
      661 gagctgggcg aggaggtggc cgcgttcgtg acgctgcggc cgggcacgag cgcggaggcg
      721 ccggagctga tcgcgttctg ccgggagcgg ctggccggct acaagtatcc gcggcgcgtg
      781 acggtggtgg acgcgatgcc gcgcagcgcg accggcaaga tcctgaaggc gaagctgcgc
      841 tagggaggcg agcggcgtgg aggtgctccc cccacgccgc ccctatgtga acgcgcgacg
      901 ctcgccgcgc ggctggttgg cgctacttct tgggctgctc gggcgtcttg gtgtcgggag
      961 ccttcgccgc cgagtcaccc tcgctcttct tggccgcctt cttcttggcg gtgtgcttgg
     1021 ccttgccgtc cttcttgtcc atcttgtcca tctggctgtc gcccttcgac ggagccgccg
     1081 gcgcgggcgt cgtgctcggg gccggagccg gggtctgcgc gagcgcggga ccggccaccc
     1141 cgagggcaaa caccgttgcg gccaccaggg acatcacgtg attcgtcttc atggaaaact
     1201 cctctctctt cattgagggt tggtttgtga gttattggaa caggttcgtg aagaagctct
     1261 tcacgctgtg gccgaacgat acggcccctt ccttggttct actccaggcc gacttggcct
     1321 cgggctccgc tgcccgtccc gcctccttga ctcgctctcc cgccgtctcg gcgccgccga
     1381 ccaccgtctt gccgataccc ttcgcggtat cctcgacgcc gcgcccgacc tgcttggcgc
     1441 cgctctcgac ctgtgcggca ccttcccgcg ccttggtgtc gtccgccgcg ccggcgggcg
     1501 ccgccgccac gatcagcgcc agcagtgcga cgaatgaggt gagccgactc ttcactgccc
     1561 tctgcatctc catcctccct ggtcgtgggc ctgtctcgtg ggtgtaactt tgtgatccac
     1621 gagaaagttc accggcaacc cacatgccag cgaagatcgg gagcgggcga ggcgtatcga
     1681 ccgtctgtgc ctcgattggc gaggcgcggc gacgagcccg tgggaggaat tacacggcgg
     1741 cgtgtaagga ttaccaggag aagacgtgcg tgggcgtcag ctcgacgatg accgaatcgg
     1801 tctcgccgag cgcggcctcg cgcggatact gcggatactt tgcgtagagg cgacggcgca
     1861 ggccgcggaa gcgcgggccg cgctcgatca cgcgcgcgcg gccctgcacc atcaccccgg
     1921 tgatgtgggc ccagtcgtcc gagtagagat cgacggtgag ggcgagccga gggttcgcgc
     1981 gcacgttgag gaccttgcgg cccttgcggc ccgagccgaa gtagatcttc ccgcccgcca
     2041 gcacgtggca gaccggcacc agatgcggca tcccgtcgcc gacggtggcg acgcgcgcca
     2101 cccgttcccg ctcgagcatc ttcgcctgcg ccttcgtcag tcgcatgtgt ccctcctgtg
     2161 aagatcgagt gtactgcacg ggcgcattga cccccggtga acgcttgatg tagattccac
     2221 cgcatgaaaa gcaaggcatc gaccaagaag tccaagacgt cgtccgctcg cgccaaggcc
     2281 aagccccagc cgaagccggc ggcccggaag gcggcggcca aggcccccgc ggcccgggcc
     2341 accgtcgccg cggcccccaa ggcccaggcc tacaccccga agccgatcga ggggatcggc
     2401 tgggcgccgt tccgctacag ccggtagctc ctccctcgcg cgcccggagc gccgggcgcg
     2461 cccgggtcgg gggcgtggcc gtgctgggcg ccctcctcct gctgcccctg gccgccgggt
     2521 gcgcgccgct ggagcggctc ggccagaact ggtgggagct tcgccgggac tcctccggcc
     2581 aggcccccga tcccgcctcc accccggacg cggtcatcca ggtctacgcg gcccgcacgg
     2641 tcggctggcg tggagtgatg gccgttcaca cctggctgac cctcaagccg gccggggcgc
     2701 cggcctatac ccgctacgag gtgatgggct ggggggtcgg caacggggcg cccgccatcc
     2761 gggtgaaccg cagcgggccc gacaactact ggttcggcag ccggcccgag ctgctggtgg
     2821 accggcgggg ggaaggcgtc gacgggctga tcacccgcgt ccaggccgcg gtcgagagct
     2881 atccctacgc ggccagctac cggacctggc cggggcccaa cagcaacacg ttcaccgcgt
     2941 acctggggcg ggccgtcccc gagctacgcc tggatctgcc cgccaccgcc atcggcaagg
     3001 acttcatccc gggcgggctg ccggcggcca tggccccatc gggcaccggc gtgcagctgt
     3061 ccctcttcgg cctgctgggc gtgctggccg gcgcggaaga ggggctcgag ctgaacgtgc
     3121 tgagcctgac cttcggggtg gacgtgaagc ggccggcgct caagctgccg gtggtgggcc
     3181 gcctgggctt cagccggcgg ccgccacccc cggcccccga gtgatcgtct cccagcccgc
     3241 ctcggccagc ggccgcgccc gctcgccgcg cgcgcgggca ttgacgtcgt tggcggtgcc
     3301 cgcgatcacc cggcccatga tgccctgggc gatggcggcc agccggaaca tcgcgaaggc
     3361 caggtagaac tcccagtccg ggatacccga gcggtcggtt cgccggcagt aggtggcgac
     3421 gtactcggcc tcggagggca ggcccatctc ggcgagcggc gcgcccagca ccccgccgag
     3481 cgtgctgggc ccgagacggt acggcatgca gttgtaggcc aggtccgcca gcgggctccc
     3541 gagggtggag agctcccagt cgagcaccgc caccacccgg ggctcggtcg gatgcaggat
     3601 caggttgccg ggccggaagt cgccgtggac gacggtgggc cgctcgtcgg cgggcacgtg
     3661 cgcgggcagc caggcgatga gctgctccat ggcctcgatg cgctcggtct cggaggcgcg
     3721 gtactgcgtg gtccagcggt ggatctgccg cgcgaagtac gagcccggcc ggccgtagtc
     3781 ggcgagcccg gttgcctcga ggtcgacccg gtggagcgtc gcgagcacct cgttcatcga
     3841 gccgtagatg caggcgcggt ccggcgggct catcccgggc aggagcgggt ccgcgaccac
     3901 gcgcccgcgc acccactcca tgatgaagaa cgcggtgccg atcaccgcgt cgtcctcgca
     3961 gagcgcgtag gtgcgcggca ccggcacccg ggtcgacgcg agcgcggtga tgatccggta
     4021 ctcgcgatcg accgcgtgcg cggacggcag gagcttgccg ggcggcttgc ggcgcagcac
     4081 gtactcgccg ccgggcgacg agagccggta ggtcgggttc gactggccgc cctggaactg
     4141 ggtcaccgtg accgggccct tgaacccccc gacgtgcccg gccatgtagc gctcgagtgc
     4201 ggccacgtcg aagcggtggg tctcgcgcac cgggatggtg tcggcggtgg agctcacctc
     4261 ggcggcgtcg cgcggggcgg agggcgtggt catggcggcg ggttcagatt atgctgcgcg
     4321 gccgcgacgg gggcaaccgc aagtttgctt cgcggcggga acttcagtcc tcgttcagta
     4381 ctcgatcacg tgatccgccc gcagcagaag agacggcggg atcttgaggc cgagcgtccg
     4441 ggcggtcttg aggttgatga cgaactcgaa cgcggtgggc tcctcgacgg gcaggtcggc
     4501 gggcctcgcc cccttgaaga tcttgtgcgc gaggccggcg gcgcgccggt acatctcggg
     4561 gtagttcact ccgtacgcga ccagtccgtg ggcgaactcg gtggaggcgt agatcgcggg
     4621 aagccgatac ttcgccgcga ggtcgacgat gacgccctgg ttcgcctggt tcagggtgtc
     4681 gagaccgacg atgagcgcgt cggcccgctg gcggatcgcg ctccggaacg ctggctccag
     4741 gtcctcgacc ttgcgcgcgt cgagcaggat gggctcgatc tccagggagc gagtcgcccg
     4801 ctcgatctct cgccactgag gtgggagcgc cgggttgctc atgttgaaga gcgccgcgat
     4861 ccgggccgcc
//
LOCUS       DATMCV010000535         8502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_26466, whole genome shotgun sequence.
ACCESSION   DATMCV010000535 DATMCV010000000
VERSION     DATMCV010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26466"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VNU03_12345"
     CDS             complement(<1..250)
                     /locus_tag="VNU03_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75301.1"
                     /translation="MASARRRAGGGPGFGQWFVLGGAVVVILGLTFALGLLVGRQLAR
                     SSLAAGPPSASEAARKVGAPAPRRGGIAAETMADRPPEP"
     gene            complement(253..1941)
                     /gene="argS"
                     /locus_tag="VNU03_12350"
     CDS             complement(253..1941)
                     /gene="argS"
                     /locus_tag="VNU03_12350"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWP75302.1"
                     /translation="MSLNITDHLADAVRQALDAAGLPAPSEVQWEIPRDARHGDYATN
                     VAMTLSREARRPPRKIAEAIVGAFPRTPVVDRVELAGPGFLNVFLSPAWCADALHEVL
                     AAGDEYGRSDAGKGARVLLEFVSANPTGPLVIVNARAAAVGDALARILRAQGYAVESQ
                     YYVNDAGNQFTALARSVDVRLRQALGEAVELPENAYPGEYLVDLVAEWLARDPAGMRA
                     LAARPEAERLEQLGRRAVADMVESQRRVLEAYGTHFDQWAHEQRDVRETSRPREVIEA
                     LTRAGHTYEQDGALWFRSTAFGDDKDRVVRRSDGELTYFGVDIGFHHFGKFGRAEHVI
                     DFLGPDHHGYVARVRAAMQALGHPSESFDVMIVQLVTLLRDGQPVRMSKRRGEFVLME
                     ELLEEVGRDAARFTFLTRRHDSPLEFDLAVATRQSSDNPVFYVQYAHARVGSLFKNAA
                     EQGISVPAWSQVDMRPLVLDEEQALIKRLLQYPDLVAGAARAREPHRVAYFLSELAGL
                     FHPYYKAHRVIVSDRAITLARLGLCAAVAQVMRNGLDLLGVSAPESMTRAAEHD"
     gene            complement(2020..3495)
                     /locus_tag="VNU03_12355"
     CDS             complement(2020..3495)
                     /locus_tag="VNU03_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034788549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HWP75303.1"
                     /translation="MRARPRARAVMLQGTGSHAGKTVLVAGLCRLLARSGLRVVPFKS
                     QNMSLNAHVAPGGGEMGWAQVMQAEAAGIAPRVEMNPILLKPRGEMRSQVVLLGRARR
                     ESDARGYYRLASTLWPAVRRSYEALAREADVVVIEGAGSPAEPNLMRRDLANMRVARM
                     ARAPVLLVGDIDRGGVFASLLGTMLLLPPVDQRRVRGFVINKFRGDPGLLAPATRDLT
                     RRTRVPVVGVVPFLRDLALPEEDSVGLDDARPPGDGLRVAVVRLPHIANFTDFAPLES
                     EPGISLVYAETGRALAEAALVILPGSKDTIADLRALKARGLGRALDAHHRRGGRILGV
                     CGGFQMLGREVVDPHHVESGGREDGLGLLPVATTLAPEKITRRVRARWLPDGPDVEGY
                     EIHVGETVADPPSRPLLEIEGRAEGCVSADGRVWGTYLHGLFESGAGRRRVLDWARGD
                     SGPWSGAARDHRALREAAYDRLADTLAACLDPRVLRELGGA"
     gene            complement(3492..4427)
                     /gene="cbiB"
                     /locus_tag="VNU03_12360"
     CDS             complement(3492..4427)
                     /gene="cbiB"
                     /locus_tag="VNU03_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HWP75304.1"
                     /translation="MTVLALAVALDLLAGDPPNRWHPVAWLGALIARGRRHAPRGVDD
                     LALYGTFMILVVAGAAAGGAVAAHAVLATLPAPVALLGDAWLLKCSLSLDGLFAAVEI
                     TRGHLVAGDLAGARRQVAWHLVSRPTEDLDAAAVASAAVESLAENLTDGLVSPVCFYV
                     AGTLLGGVGAGLALAWAYRAVNTADAMIGYRRDELEYLGRATARTDDALNYVPARLAA
                     ATIVAGAWLAGQSRAGAARVWRRDGSRTESPNAGQTMAAMAGALGVTLEKRGHYRLGD
                     GPPPDPEAIDRALRVVRRAAALGLAGAGLALLLGR"
     gene            complement(4455..4928)
                     /locus_tag="VNU03_12365"
     CDS             complement(4455..4928)
                     /locus_tag="VNU03_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWP75305.1"
                     /translation="MFPEPPSSSGSVAAGTPGTAAAPAPGPGSRSGGEAPGEGGTSGT
                     SGPALALGTPGPGAGAIPAEYGPYLQRFRQRVQESLVYPLAARRQGLRGSVELDVWLD
                     PAGRVRDVRVARSSSHGLLDDAAIETVRHLGPLPFPESLPRRALLIRLPLVFDLR"
     gene            complement(5321..5974)
                     /locus_tag="VNU03_12370"
     CDS             complement(5321..5974)
                     /locus_tag="VNU03_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HWP75306.1"
                     /translation="MISGVSVEAGREAVVLVSERSLRVLSSAVHHGGFVDARAVVNLH
                     VDKNDPCADPAGMIGAFARRAGVPAPWVGLLTGAFTERAAVALETADDLRALAITTVG
                     LSNLAAAGHSPIATWTPSTINTVVVVDADPEPAALVNAVITATEVKGLALRAAGLVVE
                     DGVAASGTSTDAVVIAATGRGARARFGGPASPLGWVVARAVRVALDRSIRDWQERAR"
     gene            complement(5985..7865)
                     /locus_tag="VNU03_12375"
     CDS             complement(5985..7865)
                     /locus_tag="VNU03_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWP75307.1"
                     /translation="MREQLVWILALSLAAPAVAAAQEQKSVDPVVITATKVATPAEQI
                     GASISVVNGDDFQTYHYPTVDEALRNVPGVEIRRSGSYGKTSSVTIRGANANQVQVLV
                     DGVRVKSPTLGQVDLSDLSPDLIERIEIIRGAQSTLYGADAIGGVVNIITRKGSGGPF
                     QASVQQEVGNYDTLVSRATVGGAWKILNYALSASHFESNGQFQNDGTDANALSARIGA
                     TLPWNSSLDFIFRYNKNDIGVPVKPVFPGPQPIVPIINPNARQQSETIIYSLEGKTRP
                     VEWWETRGRLSRYENSQGFQDPLDPGVAFDFQTFSQVDVVRKEAEWVNSFFIGKWSTS
                     SVGLEYRREEGENKGVFRTATETQSVFLEQQLRFFDRLFITGGFRVEDNSVFGTTTTE
                     RGSVAFVIKETGTRLHGSAGTGFRAPTFNDLFFPDFGNPNLQPEKSLSYDFGVDQKLW
                     NNRIRLGLTYFQNNYRDLITCCVPLPTAPFGGPINVGRARSAGIEFTSEMDVLPNLVA
                     SMNYTYTDSENLSTDRPLPREPRHRWNIGLTWEPIARLALFTQVHVVTEQFEPFGEVY
                     NSGNTRVDVGGTWRILDRTGWLQKLELTARIQNLLNEGYAEVRGFPALGINALVGLRA
                     SF"
     regulatory      complement(7914..8070)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8142..>8502)
                     /locus_tag="VNU03_12380"
     CDS             complement(8142..>8502)
                     /locus_tag="VNU03_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75308.1"
                     /translation="GERQRVMLARALAQEPRLLILDEPTAHLDLRYQAECAGLLRRLG
                     REAGLTIVLVSHDLGFAAELCDRLLLMAAGAAVRVGPPETVMDEAVLEAAYGCRVIVD
                     KHPISRRPTVHLVYPGA"
BASE COUNT         1176 a         3105 c         3098 g         1123 t
ORIGIN      
        1 tcggctcggg cggccggtcg gccatggtct ccgccgcgat gcctccgcgg cgcggcgcgg
       61 gcgcgccgac ctttcgcgcc gcctcggagg cggacggggg cccggcggcg aggctgctcc
      121 gcgcgagctg gcggcccacc agcaggccca gggcgaaggt caggccgagg atcaccacca
      181 ccgcgccgcc cagcacgaac cactggccga agcccggtcc accgcccgcc cgccgtcgcg
      241 ccgaggccat gctcagtcgt gctccgcggc gcgggtcatg ctctcgggag ccgagacccc
      301 gagcaggtcg agcccgttgc gcatcacctg ggccaccgcg gcgcagagcc cgagccgcgc
      361 cagcgtgatc gcgcggtcgg agacgatcac ccggtgcgcc ttgtagtacg ggtggaagag
      421 gccggccagc tccgagagga agtacgccac ccggtgcggc tcccgggccc gcgcggcccc
      481 cgccaccagg tccgggtact gcagcaggcg cttgatcagg gcctgctcct cgtcgaggac
      541 gagcgggcgc atgtccacct ggctccacgc cgggacggag atcccctgct cggccgcgtt
      601 cttgaagagg ctgccgaccc gggcgtgggc gtactgcacg tagaacaccg ggttgtcgga
      661 ggactggcgg gtcgccaccg cgagatcgaa ctcgagcggg ctgtcgtgcc gccgcgtgag
      721 gaaggtgaag cgggcggcgt cgcggccgac ctcttccagc agctcctcca tcagcacgaa
      781 ctcgccccgc cgcttggaca tgcgcaccgg ctggccgtct cgcagcaggg tgacgagctg
      841 gacgatcatc acgtcgaagg actcggacgg atggccgagc gcctgcatgg ccgcccgcac
      901 gcgggccacg tagccgtggt ggtcggggcc gagaaagtcg atcacgtgct cggcccgccc
      961 gaacttcccg aagtggtgga agccgatgtc caccccgaag taggtcagct ccccgtcgga
     1021 gcggcggacc acccggtcct tgtcgtcgcc gaaggcggtg gaccggaacc agagcgcgcc
     1081 gtcctgctcg taggtgtgcc cggcgcgggt cagcgcctcg atcacctcgc gcggccggct
     1141 cgtctcgcgc acgtcgcgct gctcgtgggc ccactggtcg aagtgggtgc cgtaggcctc
     1201 gagcacgcgg cgctggctct ccaccatgtc cgcgaccgcc cgccgcccga gctgctcgag
     1261 ccgctctgcc tccggtcgcg ccgcgagcgc ccgcattccg gccgggtcgc gcgcgagcca
     1321 ctccgccacc aggtcgacga ggtactcgcc ggggtacgcg ttctcgggca gctcgaccgc
     1381 ctcgccgagc gcctggcgga ggcgcacgtc caccgaccgc gcgagcgcgg tgaactggtt
     1441 gcccgcgtcg ttgacgtagt actgggactc gaccgcgtag ccctgcgcgc gcaggatgcg
     1501 cgcgagggcg tcgcctaccg cggcggcccg cgcgttgacg atcacgagcg gcccggtggg
     1561 attggccgag acgaactcga gcaggacccg cgcgcccttg cccgcgtcgc tccggccgta
     1621 ctcgtcgccc gcggccagca cctcgtgcag ggcgtccgcg caccaagcgg gcgacaggaa
     1681 gacgttgagg aagccgggcc cggcgagctc gacccggtcc accaccggcg tccgcggaaa
     1741 ggcgccgacg atcgcctccg cgatcttgcg cggcggccgg cgggcttcgc gcgagagcgt
     1801 catggcgacg ttggtggcat agtcgccgtg acgcgcgtca cgcgggatct cccactgcac
     1861 ctcggaaggt gcgggcaacc cggcggcgtc gagcgcttgc ctcactgcgt ccgcgagatg
     1921 gtccgtgatg ttcaaagaca ccgatcgccg ccgatgcggc ggcgattcat ggatgatacc
     1981 ccccggtccg cgggcgagcg aagaaagaaa tccgggcctt tacgcgccgc cgagctcgcg
     2041 cagcacgcgc gggtcgaggc acgcggcgag cgtgtcggcg agccggtcgt aggcggcctc
     2101 gcggagcgcg cggtgatcgc gcgcggcgcc ggaccacggt ccgctgtcgc cgcgcgccca
     2161 gtcgagcacg cggcggcggc ccgcgccgga ctcgaagagc ccgtggaggt aggtgcccca
     2221 cacgcgcccg tcggccgaga cgcagccctc cgcgcgacct tcgatctcga ggagtggccg
     2281 ggacggcgga tccgcgaccg tctcgcccac gtggatctcg tagccctcga cgtccgggcc
     2341 gtccggcagc cagcgggcgc gcacccgccg cgtgatcttc tccggcgcca gcgtggtcgc
     2401 caccgggagc aggccgagcc cgtcctcacg tcctccgctc tccacgtggt gcgggtctac
     2461 gacctcgcgg cccagcatct ggaacccgcc gcagacgccc aggatgcggc ccccgcggcg
     2521 atggtgcgcg tcgagggcgc ggccgagacc ccgcgccttc agcgcgcgca gatcggcgat
     2581 ggtgtccttc gagccaggca ggatgacgag cgcggcttcc gcgagggccc gcccggtctc
     2641 cgcgtagacg agggagatcc cgggctcgct ctcgagcggc gcgaagtcgg tgaagttggc
     2701 aatgtggggc agtcgcacca ccgcgacccg gagcccgtcg ccgggcgggc gcgcgtcgtc
     2761 cagccccacc gagtcttcct ccgggagggc gagatcccgc aggaacggca cgacgccgac
     2821 caccggcacc cgcgtgcgcc gcgtcaggtc gcgggtcgcg ggcgcgagga ggcccgggtc
     2881 gccgcggaac ttgttgatga cgaagccgcg cacgcggcgc tggtcgacgg gcggcagcag
     2941 cagcatggtg ccgagcaggc tcgcgaagac gccgccgcgg tcgatgtcgc ccaccagcag
     3001 caccggggcg cgggccatcc gggccacccg catgttcgcc aggtcgcggc gcatcaggtt
     3061 gggctcggcg gggctgccgg ccccctcgat gacgacgacg tcggcctcgc gcgcgagcgc
     3121 ctcgtagctg cgccgcaccg cgggccagag cgtggacgcg aggcgatagt agccgcgcgc
     3181 gtcgctctcc cgccgggcgc gacccaggag caccacctgg ctgcgcatct cgccgcgcgg
     3241 cttgagcagg atggggttca tctccacacg gggcgcgata ccggccgctt ccgcctgcat
     3301 gacctgggcc cagcccatct ccccgccccc gggcgccacg tgggcgttga gcgacatgtt
     3361 ctgcgacttg aacgggacca cgcgcagacc gctccgggcg agcagccggc acaggccggc
     3421 gaccagcacc gtcttgcccg cgtgcgagcc ggtgccctgc agcatcaccg cgcgcgctcg
     3481 tggccgggcc ctcatcgtcc gagcaggagc gcgaggccgg cgccggccag gccgagcgcg
     3541 gcggcccggc gcaccacgcg gagcgcgcga tcgatcgcct ccgggtcggg cggcggaccg
     3601 tcgcccagcc ggtagtggcc gcgcttctcg agcgtcaccc cgagggcgcc cgccatcgcg
     3661 gccatggtct ggcccgcgtt ggggctctcg gtccgcgacc cgtcgcgccg ccacacgcgg
     3721 gcggcgccgg cccgcgactg gcccgcgagc cacgcccccg ccacgatcgt cgcggcggcc
     3781 agccgggccg gcacgtagtt gagcgcgtcg tcggtgcgcg cggtggcgcg cccgagatac
     3841 tcgagctcgt cccggcggta gccgatcatc gcgtccgcgg tgttcaccgc acggtaggcc
     3901 caggcgagcg cgagacccgc gccgaccccg cccagcagcg tgcccgcgac gtagaagcag
     3961 accggcgaca ccaggccgtc ggtgaggttc tcggccagcg actcgaccgc cgcggaggca
     4021 accgcggcgg cgtccaggtc ctcggtgggc cggctgacga gatgccaggc cacctggcgc
     4081 cgcgcgcccg ccagatcgcc cgccaccagg tgccctcgcg tgatctccac cgccgcgaac
     4141 aggccgtcga gcgagaggct gcacttgagc agccacgcgt cgccgaggag cgcgaccggc
     4201 gcgggaagcg tggccagcac cgcgtgcgcg gccaccgcgc cgcccgccgc cgcgccggcc
     4261 accaccagga tcataaaggt gccgtagagg gccaggtcgt cgacaccccg gggcgcgtgc
     4321 cggcggccgc gggcgatgag cgcgccgagc cacgccaccg ggtgccagcg gttgggggga
     4381 tcgccggcca ggaggtcgag cgcgaccgcc agggccagca ccgtcatcgg ggccggcggc
     4441 ggcgcatcag cgagtcaccg gagatcgaac acgaggggca gccggatgag cagcgcgcgc
     4501 cgcggtagcg actcggggaa cggcagcggt ccgaggtggc ggacggtctc gatcgcggcg
     4561 tcgtcgagga ggccatggct ggacgaccgt gccacgcgca cgtcgcgcac ccgccccgcg
     4621 ggatcgagcc agacgtcgag ctccacgctg ccgcgcaggc cctgccggcg cgcggccagc
     4681 ggatacacca gcgactcctg gacgcgctgc cggaatcgct gcaggtaggg cccgtactcg
     4741 gccgggatcg cgccggctcc ggggcccggc gtgccgaggg ccagggccgg gccggacgtg
     4801 ccggacgtgc caccttcacc gggcgcctct ccgccggacc gcgagccggg gccgggcgcg
     4861 ggcgccgcag ccgtcccggg tgtcccggca gcaaccgagc ctgatgatga tggcggctcc
     4921 gggaacatcg gaatactcgg tagatctgat acagctggta aggaaggact cgagggtggt
     4981 gtcggcgccg cgtcggcgct gggtaccggc gggaccgggg gcggagtcgc ctgaggcatg
     5041 agcgccggtg gcggggtagc ggaaggcatc gcccgcgccg cgcccttggg cgcctcggtg
     5101 gcgcgcgacg gaggagggcc cgtcgggccg gagcggggcg gctgcgcccc ggaggctccc
     5161 cgtagcccgg cggcggtgcc gcccggcgcc tcggcgcgct cgaccagatc gacgaagagc
     5221 ggccgacccc agtccgcgac ggacagggtg aggagcagcg ccccggcccc cacgtgcgcg
     5281 accaccgaga gcgccacacc gccgacgcgg gcgaccggac tcaccgggcg cgctcctgcc
     5341 agtcgcgaat gcttcgatcg agcgcgaccc ggaccgcccg cgccacgacc cagcccagcg
     5401 ggctcgcggg cccaccgaag cgcgcgcggg caccgcgacc cgtggccgcg atcacgaccg
     5461 cgtcggtcga ggtgccggag gccgccacgc cgtcctcgac caccagcccg gccgcccgga
     5521 gcgcgaggcc cttgacctcg gtcgcggtga tcaccgcgtt gacgagggcc gccggctccg
     5581 gatcggcgtc gaccaccacc accgtgttga tcgtcgaggg cgtccaggtc gcgatcggcg
     5641 agtggccggc cgcggccaga ttgctgaggc cgaccgtggt gatcgccagc gcccgcagat
     5701 cgtcggccgt ttccagggcg accgccgccc gctcggtgaa ggcgccggtc agcagcccga
     5761 cccagggcgc gggcaccccg gcccgacggg cgaatgcccc gatcatcccg gccgggtccg
     5821 cgcaggggtc gttcttgtcc acgtgcaggt tcaccaccgc gcgggcgtcg acgaagccgc
     5881 cgtggtgcac cgccgaggac aggacgcgca gcgagcgctc gctcacgagc accaccgcct
     5941 cccgccccgc ctcgacggac accccggaga tcatcgagcc ctccctagaa cgacgcccgc
     6001 agccccacga gggcgttgat gccgagcgcg gggaacccgc gcacctccgc gtagccctcg
     6061 ttgaggaggt tctggatccg ggcggtcagc tcgagcttct gcagccaccc cgtgcggtcg
     6121 aggatacgcc aggtcccgcc gacgtccacc cgcgtgttgc cgctgttgta cacctcgccg
     6181 aagggctcga actgctcggt caccacgtgg acctgcgtga acagggcgag gcgcgcgatc
     6241 ggctcccagg tgaggccgat gttccagcgg tgccgtggct cgcgcgggag cggccggtcg
     6301 gtcgagaggt tctcggagtc ggtgtacgtg tagttcatcg aggccaccag gttggggagc
     6361 acgtccatct cggaggtgaa ctcgatgccc gcgctgcggg cgcgccccac gttgatcggc
     6421 cccccgaacg gcgcggtggg gagggggacg cagcaggtga tcaggtccct gtagttgttc
     6481 tggaagtagg tcaggccgag ccggatacgg ttgttccaga gcttctggtc gaccccgaag
     6541 tcgtaggaga ggctcttctc gggctggagg ttgggattgc cgaagtcggg gaagaacagg
     6601 tcgttgaagg tgggcgcccg gaacccggtc cccgcgctgc cgtgcagccg ggtccctgtc
     6661 tccttgatca cgaacgccac cgagccgcgc tcggtggtgg tggtgccgaa gacgctgttg
     6721 tcctccaccc ggaagccccc ggtgatgaag agccgatcga agaagcggag ctgctgctcg
     6781 aggaagacgg actgggtctc ggtcgcggtg cggaacaccc ccttgttctc tccctcctcc
     6841 cgccggtact cgaggccgac gctgctggtg ctccatttcc cgatgaagaa cgagttgacc
     6901 cactccgcct cctttcgcac gacgtccacc tgggagaagg tctggaagtc gaaggcgact
     6961 ccgggatcga gcgggtcctg gaagccctgg ctgttctcgt agcgggagag gcggccgcgg
     7021 gtctcccacc actccaccgg acgggtcttg ccctccaggc tgtagatgat cgtctccgac
     7081 tgctggcgcg cgttggggtt gatgatcggg acgatcggct gcggccccgg gaacaccggc
     7141 ttcaccggca ccccgatgtc gttcttgttg tagcggaaga tgaagtcgag ggaggagttc
     7201 cacggcagcg tcgccccgat tcgcgcgctg agcgcgttcg cgtcggtccc gtcgttctgg
     7261 aactggccgt tgctctcgaa gtgggacgcc gacagcgcgt agttcaggat cttccaggcg
     7321 ccgccgacgg tggctcggct gacaagcgtg tcgtagttgc ccacctcctg ctgcacggag
     7381 gcctggaacg gcccgccgga gcccttgcgg gtgatgatgt tgaccacgcc gccgatggcg
     7441 tcggcgccgt acagcgtgga ctgggcgccc cggatgatct cgatccgctc gatcaggtcc
     7501 ggagagaggt cggagagatc cacctgcccc agggtcgggc tcttcacccg gaccccgtcg
     7561 accagcacct gcacctggtt ggcgttggca ccgcggatgg tgacgctcga cgtcttgccg
     7621 taggagcccg agcgacggat ctcgaccccg ggtacgttgc ggagggcctc gtccaccgtc
     7681 gggtagtgat aggtctggaa gtcgtcgccg ttcaccacgc tgatggaggc gccgatctgc
     7741 tccgccgggg tcgcgacctt ggtcgcggtg atcaccaccg gatccacgct cttctgctcc
     7801 tgggccgccg ccaccgcggg cgccgccagc gacagagcca gaatccacac cagttgctct
     7861 cgcatcgacc actccttttg cctcgaaagg ggttggcgct gctgatcgca cccaggtctc
     7921 ctgactcgtg gatcggcgtc gtggcccggc cttcccaggc gcgccctcgg gcggcccagt
     7981 ggctccctcg cgggatcggg cgggactccc cactcacagt ggcgggaccg tgccggcttc
     8041 ggcccgttca tcgggccacc ggcttccctt ggagtgcgat ctgttttctt gcgctagctc
     8101 actttaggtg tcatcacctc cttcgttact ctcggcctga atcatgcgcc cgggtacacc
     8161 aggtgaaccg tggggcgccg gctgatcgga tgcttgtcca cgatcacacg gcagccgtag
     8221 gcggcctcga gcacggcctc gtccatcacc gtctccggcg ggccgacccg gaccgcggcg
     8281 cccgcggcca tgagcaggag ccggtcgcag agctcggccg cgaaccccag atcgtgcgag
     8341 acgaggacga tggtgaggcc cgcctcacgg ccgagccggc ggaggaggcc ggcgcactcg
     8401 gcctggtagc gcaggtccag gtgggccgtc ggctcatcga gaatcagcag ccggggctcc
     8461 tgggccaggg cccgggccag catcacccgc tgccgctccc cc
//
LOCUS       DATMCV010000536         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_762842, whole genome shotgun sequence.
ACCESSION   DATMCV010000536 DATMCV010000000
VERSION     DATMCV010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762842"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /gene="lexA"
                     /locus_tag="VNU03_12385"
     CDS             complement(<1..538)
                     /gene="lexA"
                     /locus_tag="VNU03_12385"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWP75309.1"
                     /translation="MTARQREVLEFIRVFGERHGVPPTVREIGEKFDFTARAAFDHLK
                     ALERKGMLERRVTGKRASRTLVLPSQKGPRRLDPIGIPVVGRIAAGLPITAVENHEDT
                     IPLRPDWLATGGQDVFALRVRGDSMIDAHIVDGDLVLVRKQETAGSGDIVAAMLDGEA
                     TVKRFGREGGAVVLRPEHP"
     gene            complement(627..1100)
                     /locus_tag="VNU03_12390"
     CDS             complement(627..1100)
                     /locus_tag="VNU03_12390"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWP75310.1"
                     /translation="MFDIGLQELVLIFVIALLVFGPKNLPQLGRSLGRAMREFRRASD
                     EFRSTIETNLKINEPDPLPEPPPAAALEPAPAAVATTEALPESTLNPYDTPAIPVPEG
                     EAYVAQRGGKLFHGRDCGWLRRIAEPERVYFKRVADAREAGFAACPSCEPWEPAG"
     gene            1345..>2109
                     /locus_tag="VNU03_12395"
     CDS             1345..>2109
                     /locus_tag="VNU03_12395"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWP75311.1"
                     /translation="MTRWTATAVLAVVMATIQPVAATAQTSDAVGVVTTIDGRATVAR
                     PAAPSPLSLKFKDDVFGRDRISTQENSLVRVLLGGKAILTVRELSEVTISEEPGRAVV
                     TLPSGKVVLAVQKQRMRPGESIEIRTPNAVAAVRGSIVSVGYDAVLQQTTAICHTGDV
                     TYQYLGGAIVPLPPGAGARNGLLGSVSMAERIAAVNVEPGAHTFSAPDEGFQTSLFQG
                     ETGKAVQVAQFASTGTLPGTPNLNPSPTAPLNALSSH"
BASE COUNT          339 a          760 c          702 g          308 t
ORIGIN      
        1 tcgggtgctc cggccgcagc acgaccgcgc cgccctcccg cccgaagcgc ttcacggtgg
       61 cctcgccgtc gagcatggcg gccacgatgt ccccgctccc cgcggtctcc tgcttgcgca
      121 ccagcacgag atcaccgtcg acgatgtgcg cgtcgatcat gctgtctccg cggacgcgca
      181 gggcgaagac gtcctgccca cccgtggcga gccagtcggg ccggagcgga atcgtgtcct
      241 cgtggttctc gaccgcggtg atcggcaggc ccgccgcgat ccggccgacc actgggatcc
      301 cgatcgggtc cagccgacga ggccccttct ggctggggag caccagcgta cgggatgccc
      361 gcttgccggt gacccgccgc tcgagcatgc ccttccgctc gagcgccttc agatggtcga
      421 aagcggcgcg ggccgtgaag tcgaacttct cgccgatctc gcggaccgtg ggaggcacgc
      481 cgtgccgctc tccgaagacg cggatgaact ccagtacctc gcgctgccgg gctgtcatct
      541 ctcgcaccgt ggcccccctc tctcgatcga gcctcaacag gatgatagtc aacacccgtt
      601 gagtccggca agcaaaaaaa ggggggctac ccggcaggct cccagggctc gcaggatggg
      661 caggcggcga agccggcctc ccgggcgtcg gccacccgct tgaagtagac ccgctccggc
      721 tcagcgatcc gccggagcca gccgcagtcg cggccgtgaa agagcttccc tccgcgctgc
      781 gccacgtagg cctcgccttc cggcaccgga atggcgggcg tgtcgtatgg gttgagggtc
      841 gactccggca aggcctcggt cgtcgcgacg gccgccgggg ccggctcgag cgccgcggcc
      901 ggcggcggct ccgggagcgg gtccggctca ttgatcttaa ggttcgtctc gatcgtcgac
      961 cggaactcgt cgctggcccg ccggaactca cgcatcgcac gcccgaggga tcggcccagc
     1021 tgcggaagat tcttcggccc aaagaccagg agggcgatga cgaagatgag aaccagctcc
     1081 tgcaggccga tgtcgaacat gagtgggtct gctcaggctc ctcaggctgc gatgatatca
     1141 ccccacgcga ggccggccgc cgagcgcaac ttgtgcgcgt tctcggcttc tggaccacga
     1201 ccggccttcc cgacggggcg agtcgctacg cagagcggat tctagcacgc gcttctcctt
     1261 gcgcgggccc aacgctccac gcataatcag gcactcggtc gcggggccga gatacattcc
     1321 cacgtcggta cggaggcgga aacgatgaca cgctggacgg caaccgcagt gctcgcagtg
     1381 gtgatggcta cgatccaacc ggtcgcggcc accgcacaaa cctccgacgc cgtcggtgtc
     1441 gtcaccacga tcgacgggcg cgccaccgtc gcgcggcccg cggccccttc gccgctctcg
     1501 cttaagttca aggacgacgt cttcggccgc gatcgcatca gcacccagga gaactcgctc
     1561 gtgcgcgtgc tgctgggcgg gaaggcgatc ctcaccgtcc gcgagctctc cgaggtcacg
     1621 atcagcgagg agccgggccg ggcggtggtc accctgccga gcggcaaggt cgtcctcgcg
     1681 gtacagaagc agcgcatgcg gccgggcgag tccatcgaga tccgcacccc gaatgcggtg
     1741 gcggcggtgc ggggcagcat cgtctcggtc ggctatgacg cggtcctgca gcagaccacg
     1801 gccatctgcc acaccggcga tgtcacctac cagtacctcg ggggagccat cgtcccgctg
     1861 ccgcccggcg cgggcgccag gaacggactg ctcggctcgg tctccatggc tgagcggatc
     1921 gcagcggtga acgtcgagcc aggcgcgcac acgttcagcg cgcccgacga agggttccag
     1981 accagcctct tccagggcga gacaggcaag gcggtccagg tggcgcagtt cgcgagcacc
     2041 gggactctac ccggcacgcc caacctgaac cccagcccca ccgcgccgct gaacgccctg
     2101 agcagccat
//
LOCUS       DATMCV010000537         5518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_896700, whole genome shotgun sequence.
ACCESSION   DATMCV010000537 DATMCV010000000
VERSION     DATMCV010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896700"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VNU03_12400"
     CDS             complement(<1..708)
                     /locus_tag="VNU03_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWP75312.1"
                     /translation="MSSPRLLPLCLLVLLASACATPAPDTLRKIRETGSVTLGYRESS
                     PPFSSVGDRGEPVGYSIDLCTRVVTQVQAVTGRSDLQIKWVRVTPDDRIAAVVDGRVD
                     LECGSTTASLSRQEQVDFSYPTFVDGGSLLVAADAGVRTPADLAGKPIAVIPKTTTEG
                     ALAAWLAQQGVQAQIIPVREHAEGFAAVAEGRAVAYASDRTLLIGLGLRYQGSRRLVV
                     LDDFLSYEPYGLMLRRSD"
     gene            complement(719..1306)
                     /locus_tag="VNU03_12405"
     CDS             complement(719..1306)
                     /locus_tag="VNU03_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75313.1"
                     /translation="MRPRTLPAIRAACAALLGMWLAGCSLASSTGATAPAGITQQLLI
                     RSLERALADMDLARIGSRPVALEVAVQSGNEGFVKDFVTTWLKAHGVRLAGDSPELKL
                     KMFVSVYGTDHDQTLIGVPAFQAPVVNVPVPELAFFKWVRNRGQTELRLWAFDGKGDV
                     VVDAPHPGVGRSKHDDFTVLLFFGFSLSDVDKRGN"
     gene            complement(1320..1979)
                     /gene="msrA"
                     /locus_tag="VNU03_12410"
     CDS             complement(1320..1979)
                     /gene="msrA"
                     /locus_tag="VNU03_12410"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWP75314.1"
                     /translation="MFGLRKRSMPSAEEALPGRRERIPVPDTHFVSGARLEPPFPAGT
                     ERAVFGLGCFWGAERKFWEVPGVYTTAVGYAGGHTPNPTYEEVCSGATGHTEAVLVVF
                     DPAKVSYESLLRVFWEDHDPTQGMRQGNDVGTQYRSAIYYYGDAQRRAALASKEAYQA
                     VLTKAGYGEITTEIQEAPEFYYAEDYHQQYLAKNPWGYCGIGGTGVACPAGLAQSATS
                     S"
     gene            2052..3026
                     /locus_tag="VNU03_12415"
     CDS             2052..3026
                     /locus_tag="VNU03_12415"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWP75315.1"
                     /translation="MTDNGKTVASPGKPAGGWQWERVLHGIVPPLISPLDDAGAPDAA
                     AMAVLVEHVVGAGCTGLFMLGGCGEGAWLTPTQRGTVIGQAVRAAAGRVPVLAGVMMP
                     GTGPAIEAARQAAGEGADALVVGSPYYFGVDAAAQERHVRDVLAAVPLPALLYNIPQC
                     THHVLAPATVRALARDPRVLGIKDSAGDFEAFQTLLALKEERPRFRVLQGHESLAAAS
                     LLQGADGMVPGLANVVPALFVELQAAVDRGEAARCAELQRRIAALAVLYRQGPWLAAL
                     KAACAMRGIGNGRPAPPLSPATPGDRAVVKALLEEAGAAEPALATPGR"
     gene            3023..3661
                     /locus_tag="VNU03_12420"
     CDS             3023..3661
                     /locus_tag="VNU03_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWP75316.1"
                     /translation="MKLQAVIFDMDGVLVDSEPFGFEALRRVMALHGFPYGEEENAEF
                     LGRTTLDSCRILRARHGIPESEETLADRYVEAMLDQIARGPIPMVGVPEVLRGVRAAG
                     YRMALASSAELRVIDANLAALSLRPLFEAVVSGTQVPRGKPAPDVFLAAAERLGVPAA
                     TCLVVEDSRNGLLAAKAAGMRCAVVPCAQTLHQEFGEADHRLAALPELLALL"
     gene            complement(3696..4412)
                     /gene="mtgA"
                     /locus_tag="VNU03_12425"
     CDS             complement(3696..4412)
                     /gene="mtgA"
                     /locus_tag="VNU03_12425"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HWP75317.1"
                     /translation="MRTRAGWAGRLAMLAIVAAGIWIGVLIWTWPSVGPLATARPTTT
                     AFIEQYRAEQRAAGRSDRAAWTWTPYAAISPALKRAVLVSEDVNFFSHHGFALDEMQN
                     ALEDALRDGELPRGASTITQQLAKNLWLSRSRNPLRKAREALLTWQLERQVSKRRILE
                     LYLNVVEFGPGIYGVGAASQRYFGKAPADLDDGEAAQLAAVLPNPTSWRPGSPSPTYR
                     RRVAAIQRRMERAGFLWKLI"
     gene            4437..>5518
                     /locus_tag="VNU03_12430"
     CDS             4437..>5518
                     /locus_tag="VNU03_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWP75318.1"
                     /translation="MDALKQRVAAAVERLGDQLTALSLDIHAHPELAFQEAHAADRVG
                     RFLAEEGFKVEAGVGGIPTAFRASLETGPGPTLAILCEYDALPGVGHACGHNVIAAAG
                     AGAGAALMAARDGLPPGRVVVIGTPAEERGGGKGCLVEAGLFRDVDAAMMIHGFDRTL
                     LHQDLLGVARVTFEWEGRASHASVDPWEGVNALDACVATFNGVAMLRQQMRPDCRIHG
                     VVAEGGIAGNIIPERAVADFNVRGPSLEAMWALHRRVVACAEAAAVATATRLTVTRHS
                     DVYEPMRRNQGLLDVFAANLGASGLAEGPAAPDRLASSDIGNVSQVTPTIHAWVQIAA
                     LGTAIHTREFARAAAAPSARLGLLAGA"
BASE COUNT          724 a         1960 c         2081 g          753 t
ORIGIN      
        1 atcgctgcgg cggagcatca gcccgtaggg ctcgtaggag aggaagtcgt ccagcaccac
       61 gaggcgccgc gatccctggt agcggaggcc gaggccgatg aggagcgtgc ggtcggaggc
      121 gtaggcgacc gcccggccct ccgccaccgc ggcgaagccc tccgcgtgct cccgcaccgg
      181 gatgatctgg gcctgcacgc cctgctgggc gagccacgcg gcgagcgcgc cctcggtggt
      241 ggtcttgggg atcaccgcga tcggcttgcc cgcgaggtcg gccggggtcc gcacgccggc
      301 gtcggcggcc accagcaggc tgccgccgtc cacgaaggtc ggatagctga agtcgacctg
      361 ctcctgacgg gagaggctag cggtggtgga gccgcactcg agatcgacgc ggccgtccac
      421 caccgccgcg atgcgatcat cgggcgtcac gcggacccac ttgatctgca ggtcgctgcg
      481 cccggtcacg gcctggacct gggtgaccac ccgcgtgcac agatcgatcg agtagcccac
      541 cggctcgccg cggtcgccca cggaggagaa cggcggcgag gattcgcgat agcccagggt
      601 cacgctcccg gtctcgcgga tcttgcgcag ggtgtcgggc gcgggcgtcg cgcacgcgga
      661 cgcgagcagg acgaggaggc agagcgggag gagccgcggg gaagacatgg ccgtcacctc
      721 agttgccgcg cttgtcgacg tcggagagcg agaagccgaa gaagagcagc accgtgaagt
      781 cgtcgtgctt cgaccgaccc acccccgggt ggggcgcgtc cacgacgacg tcgcccttgc
      841 cgtcgaaggc ccagagccgc agctcggtct ggccgcggtt gcgcacccac ttgaagaagg
      901 cgagctcggg caccgggacg ttgaccaccg gggcctggaa ggcgggcacc ccgatcaggg
      961 tctggtcgtg atcggtcccg tagaccgaga cgaacatctt gagcttcagc tcgggcgagt
     1021 cgcccgccag ccgcaccccg tgagccttga gccaggtggt cacgaagtcc ttcacgaagc
     1081 cctcgttgcc ggactggact gcgacctcga gcgcgaccgg gcggctgccg atgcgcgcca
     1141 gatccatgtc cgcgagcgcg cgctcgagcg agcggatcag gagctgctgg gtgatgccgg
     1201 cgggggcggt ggcgccggta ctgctcgcga gggagcagcc ggccagccac atccccagca
     1261 gcgcggcgca cgccgcgcgg atcgcgggaa gcgtccgggg tcgcatccga tcgagcggcc
     1321 taggacgacg tcgccgactg ggcgagaccc gccgggcagg cgacgccggt gccaccgatg
     1381 ccgcagtagc cccagggatt cttggccagg tactgctgat gatagtcctc ggcgtagtag
     1441 aactcgggcg cctcctggat ctcggtggtg atctcgccgt agcccgcctt cgtgaggact
     1501 gcctggtagg cctccttcga ggccagggcg gcgcggcgct gggcgtcgcc gtagtagtag
     1561 atcgccgagc ggtactgggt gccgacgtcg ttaccctgac gcatgccctg cgtcgggtcg
     1621 tggtcctccc agaacacgcg cagcaggctc tcgtacgaga ccttggccgg gtcgaacacc
     1681 accagcaccg cctcggtgtg gccggtggcg ccgctgcaga cctcttcgta ggtcgggttc
     1741 ggagtgtggc cgcccgcgta gccgaccgcg gtcgtgtaca cgcccggcac ctcccagaac
     1801 ttccgctccg cgccccagaa gcagcccagg ccgaagaccg cgcgctcggt gccggcgggg
     1861 aagggcggct cgagccgcgc gccgctgacg aagtgggtat cgggtaccgg gatccgctcc
     1921 cggcgcccgg gaagcgcttc ctcggcggac ggcatgctgc gcttgcgcag accgaacatg
     1981 ggctggggcc ctccttgtcg ctagagccta cctcagggca ggtcgcgagg ccactctgct
     2041 atgctccggg catgacagac aatggaaaga ccgtcgcgtc accgggcaag ccggcggggg
     2101 gatggcagtg ggagcgggtg ctgcacggca tcgttccgcc actgatctcg ccgctcgacg
     2161 atgcgggcgc ccccgacgcg gccgcgatgg cggtcctggt cgagcacgtg gtgggcgcgg
     2221 ggtgcaccgg gctcttcatg ctgggcggct gcggcgaggg ggcctggctc acgccgaccc
     2281 agcgcggcac cgtgatcggg caggcggtgc gggcggccgc ggggcgtgtg ccggtgctgg
     2341 cgggtgtgat gatgcccggc accgggcccg cgatcgaggc ggcgcgccag gccgccgggg
     2401 aaggggccga cgcgctggtg gtcgggtcgc cctactactt cggagtcgac gcggcggccc
     2461 aggagcgtca tgttcgcgac gtgctggccg cggtcccgct gcccgcgctg ctctacaaca
     2521 tcccccagtg cacgcaccac gtgctggcgc ccgcgacggt acgggcgctg gcccgcgatc
     2581 cgcgggtgct cggcatcaag gactcggcgg gcgacttcga ggccttccag acgctccttg
     2641 cgctcaagga ggagcgtccc cggttccgcg tgctccaggg ccacgagtct ctcgcggccg
     2701 cgagcctgct gcagggggcc gacggcatgg tcccgggcct tgccaacgtc gttcccgcgc
     2761 tcttcgtcga gctgcaggcc gcggtcgacc gcggcgaggc cgcccggtgc gcagagctcc
     2821 agcggcgcat cgcggcgctg gccgtgctgt accggcaggg gccctggctc gccgcgctca
     2881 aggccgcgtg cgccatgcgc gggatcggca acggccgtcc cgccccgccg ctctcgcccg
     2941 cgaccccggg cgaccgtgcc gtggtgaagg cgctgctcga ggaggcgggc gccgcggagc
     3001 ccgccctggc caccccgggc cggtgaagct ccaggccgtc atcttcgaca tggacggtgt
     3061 gctggtcgac tccgagccct tcggcttcga ggccctgcgc cgggtgatgg ccctccacgg
     3121 gttcccctac ggggaagagg agaacgccga gttcctcggg cgcaccacgc tcgactcgtg
     3181 ccggatcctc cgggcgcgcc acgggatccc cgaatcggag gagacgctgg ccgaccggta
     3241 cgtcgaggcg atgctcgatc agatcgcccg cgggccgatc ccgatggtgg gggtgccgga
     3301 ggtgctgcgc ggcgtgcgcg cggcgggcta ccggatggcg ctggcttcct cggccgagct
     3361 gcgggtgatc gacgccaacc tcgccgcgct ctcgctccgg ccgctcttcg aggcggtggt
     3421 ctccggcacc caggtcccgc gcggcaagcc cgcgcccgac gtgttcctgg ctgctgccga
     3481 gcggctcggc gtgcccgccg cgacgtgcct ggtcgtcgag gactcgcgca acggtctcct
     3541 cgcggcgaag gccgcgggca tgcgctgcgc ggtcgttccc tgcgcgcaga ccctccacca
     3601 ggagttcggc gaggccgacc accggctggc cgccctcccc gagctgctcg cgctgctctg
     3661 agcggccgac gccccgcggc gcgcatcggc cgccatcaga tgagcttcca caggaagccg
     3721 gcgcgctcca tccgccgctg gatggccgcc acgcggcgac gataggtcgg gctcggcgag
     3781 ccggggcgcc agctggtcgg gttcggcagc accgcggcga gctgggccgc ctcgccgtcg
     3841 tcgaggtcgg cgggtgcctt gccgaagtac cgctggctcg ccgcacccac cccgtagatc
     3901 cccggcccga actcgaccac gttgagatag agctcgagga tccgccgctt cgagacctgg
     3961 cgctcgagct gccaggtgag cagggcctcc cgggccttcc ggagcgggtt gcgcgagcgc
     4021 gagagccaga gattcttggc gagctgctgg gtgatcgtgg aggccccgcg cggcagctcg
     4081 ccgtcgcgca gggcgtcctc gagcgcgttc tgcatctcgt cgagcgcgaa gccgtggtgc
     4141 gagaagaagt tgacgtcctc ggacacgagg accgcccgct tgagggcggg ggagatggcc
     4201 gcgtagggcg tccacgtcca ggccgcccga tccgatcgcc cggccgcgcg ctgctcggcg
     4261 cggtactgct cgatgaacgc ggtcgtggtg gggcgcgcgg tggcgagggg gcccaccgac
     4321 ggccaggtcc agatcaggac gccgatccag atccccgccg ccacgatcgc cagcatcgcg
     4381 aggcggcccg cccatccggc ccgggtgcgc atgggtcatg gtatcgtggt ggccgcatgg
     4441 acgccctcaa gcagcgcgtc gcggccgcgg tggagcggct gggcgaccag ctcaccgcgc
     4501 tgtcgctcga catccacgcc caccccgagc tggccttcca ggaggcccac gccgccgacc
     4561 gggtcgggcg gttcctcgcc gaggaggggt tcaaggtgga ggccggagtg ggcggcatcc
     4621 cgaccgcgtt ccgggcgagc ctcgagaccg gcccggggcc cacgctcgcg atcctgtgcg
     4681 agtacgacgc cctgccggga gtcggccacg cctgcggtca caacgtcatc gcggcggcgg
     4741 gcgccggcgc gggcgccgcg ctcatggccg cgcgcgacgg cctgccgccg ggccgcgtgg
     4801 tggtgatcgg cacgcccgcg gaggagcgcg gcggcggcaa gggctgcctg gtggaggcgg
     4861 ggctcttccg ggacgtcgac gcggcgatga tgatccacgg cttcgaccgg accctgctcc
     4921 accaggacct gctcggggtg gcccgggtca cgttcgagtg ggagggccgg gcctcgcacg
     4981 cctcggtgga tccgtgggag ggtgtcaacg ccctcgacgc gtgcgtcgcc accttcaacg
     5041 gggtggccat gctccggcag cagatgcggc cggactgtcg catccacggc gtcgtcgccg
     5101 agggggggat cgcgggcaac atcatcccgg agcgggcggt ggccgatttc aacgtgcgcg
     5161 ggccgagcct cgaggcgatg tgggccctcc atcgacgcgt ggtcgcgtgc gccgaggcgg
     5221 ccgcggtcgc caccgcgacc cggctcaccg tgacccggca ttccgacgtc tacgagccca
     5281 tgcggcgcaa ccagggtctg ctcgacgtgt tcgcggccaa cctgggcgcg agcgggctcg
     5341 ccgaggggcc cgcggcgccc gaccggctgg cctcctccga catcggcaac gtgagccagg
     5401 tgacgcccac cattcatgcg tgggtgcaga tcgccgcgct cggcaccgcg atccacaccc
     5461 gcgagttcgc gagggccgcg gcggcgccct ccgcgcgcct gggcctcctg gccggggc
//
LOCUS       DATMCV010000538         4989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_560878, whole genome shotgun sequence.
ACCESSION   DATMCV010000538 DATMCV010000000
VERSION     DATMCV010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560878"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VNU03_12435"
     CDS             complement(<1..673)
                     /locus_tag="VNU03_12435"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HWP75319.1"
                     /translation="MGKRIVVNAGVTETRLAVQDGNLLTELYVERADRRSIVGNIYKG
                     VVTNVLPGMQAAFVDIGLTKDAFLYAGDYTANLGQAEPAPEAPDAEDESADVDVGDAE
                     AETEAEPRREAVGPIEEMLRKGQEVLVQVSKEPLGTKGARVTSFVSIPGRSIVYMPQA
                     RHVGVSRRIHDDAERDRLRAIVKGLPPPPGGFIVRTVAEGKGDEELVADIQFLSRLWG
                     QVQARF"
     gene            complement(725..1816)
                     /gene="rodA"
                     /locus_tag="VNU03_12440"
     CDS             complement(725..1816)
                     /gene="rodA"
                     /locus_tag="VNU03_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWP75320.1"
                     /translation="MLRLDRRLLQNVDWLLLGAALFIIGLSLTCLWSLNPGRGISSLV
                     WRQLSWVGVGLLGLLVVASVDYRNLVRSAPVLYVLGLGLLTSVFVLGRTVSGARRWIH
                     LGPFTVQPSELFKLIFIITLAWALTSGRGERFSRGMLIGIFVLLGVPFLLVVRQPDLG
                     TALVLVPVLAAILVGIGIRLKVLGALSLAGVAAMPLAWLVLKPYQRDRLLVYLDPFRD
                     PLGTAYNVIQAKIAIGSGQLLGKGMGGATQSRLSFLPERHTDFIFAVFAEMWGFLGCL
                     VLIVAYVLLVMRGFEIAAGTREMRGRILALGVTTVFAAQILINIGMVTGLLPIVGIPL
                     PLMSYGGSSMVVSLTGLGLLLSIRMRQFH"
     gene            complement(1816..3621)
                     /gene="mrdA"
                     /locus_tag="VNU03_12445"
     CDS             complement(1816..3621)
                     /gene="mrdA"
                     /locus_tag="VNU03_12445"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWP75321.1"
                     /translation="MALNPSPANRREAWGRRVLALAVVTAIGFVGLVGQLWYLQVLEG
                     GRMQELSEKNRIRVRPVAAPRGILFDRNGLALVDNRPAFTLSLIPREMEDRNSVVARL
                     AVLLKMPVGDLMDALERVPADSIRPVRVRRGLTLEDVTKVEERKLELPGVIVEVEPQR
                     VYPTSTFAAHLLGYVREVSDEQMKQGRYRRGDMIGQSGLERLLDEYLRGRDGGERIEV
                     DALGRPVQVMRREEPDPGAQVVTTIDRRIQEVAEQAMAGKAGSVVVMDPRNGDVLALT
                     SSPAFPLDRLTGNIDRDEWLRLVKDPMTPLMNRALQGQYPPGSVFKIIVAAAGLQEGS
                     LAPMDRIYCNGEFSLGHWTFKDWKAGGHGHVDLRTAVAQSCDVYFYQAGLKVGGDTMA
                     RYAQAFGLGAPTGIDLGGERFGLVPFAAARPRNKRPWQSGDTVNMSIGQGQLLVTPLQ
                     VARMMGAVANGGILWKPRLVQRVAKADGSLAYSATSKMTERVELSPVVWAFLRNALSA
                     VVKEGTGAAARLPGLDIAGKTGTAQTIAKSDSAKGQDHAWFASFAPADDAQAVVVVFV
                     ERGGHGGDVAAPIARKIYEALFLQKVAQVTLPEPG"
     gene            complement(3612..4106)
                     /gene="mreD"
                     /locus_tag="VNU03_12450"
     CDS             complement(3612..4106)
                     /gene="mreD"
                     /locus_tag="VNU03_12450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWP75322.1"
                     /translation="MLRLLVLLVIVGGGVLQSTVVAVAGVAGITPDIPLILTVLIALR
                     YGPESGCLAGFVAGLLQDATGGGLVGVQALTKGLAGFGMGLLVGRFWVSNPLVQVPGL
                     VLLTISEGLGRFLLLQMFHYPAAFGDLMLHVILPQALYNGLIGTLCILAEASFELVRR
                     RTWR"
     gene            complement(4110..4949)
                     /gene="mreC"
                     /locus_tag="VNU03_12455"
     CDS             complement(4110..4949)
                     /gene="mreC"
                     /locus_tag="VNU03_12455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWP75323.1"
                     /translation="MRVGRYGILLAVLLVSLLLLTIQTRGGGTGRAGELVTIAVTPVQ
                     SLLVRIHRGALNFWANYVDWKSVRRDNATLHEENEHLRVQAMQAGETREENARLRRLL
                     VLKDRLPLATVAGEVIGREAGGWVRSLTVNRGRGDGIAQQTPVIVPDGLVGRVVQVHR
                     GAAVIQLLNDPASTVGAVVQRTRTAGLVEGDAGGTVRFKFMARDGASVAPGDLVVTSG
                     LGTLFPKGLPVGRVIKIEDKGSALFHFAVLAPAVDFSRVEEVLLVTGQTTQDVATLFP
                     SEG"
BASE COUNT          816 a         1803 c         1695 g          675 t
ORIGIN      
        1 cgaagcgcgc ctgcacctgg ccccagaggc ggctcaggaa ctggatgtcg gccaccagct
       61 cctcgtcgcc cttgccctcc gcgacggttc gaacgatgaa tccgccgggc ggcgggggca
      121 ggcccttgac gatggcgcga agccggtcgc gctccgcgtc gtcgtggatg cggcgtgata
      181 cgcccacgtg ccgcgcctgg ggcatgtaga cgatggagcg gcccgggatc gagacgaagg
      241 acgtgacccg cgcgcccttg gtgccgagcg gctccttgga gacctggacc agcacctcct
      301 ggcccttgcg cagcatctcc tcgatcggcc ccaccgcttc ccgccggggc tcggcctcgg
      361 tctccgcctc cgcgtcgccc acgtccacgt cggcgctctc gtcctcggcg tcgggtgcct
      421 ccggcgcggg ctcggcctgg cccaggttcg cggtgtagtc gcccgcgtag aggaaggcgt
      481 ccttggtgag cccgatgtcc acgaacgcgg cctgcatccc gggcagcacg ttggtgacca
      541 cgcccttgta gatgttgccc acgatcgagc gccggtccgc gcgctcgacg tacagctcgg
      601 tgaggaggtt tccatcctgg acggcgagac gcgtttcggt cacaccggcg ttgacgacga
      661 tgcgctttcc cacggccaac ctcgatccgg ggtctcgcgg gagacccggc cggcagttct
      721 gcggttaatg gaactgccgc attcggatcg agagcaggag tcccaggccc gtcagcgaga
      781 ccaccatgga ggatccgccg tagctcatca gcggcagcgg tatccccacg atggggagca
      841 ggcccgtcac catcccgata ttgatcagga tctgggccgc aaacacggtc gtcaccccga
      901 gagccaggat gcgcccgcgc atctcacgcg tgccggccgc gatctcgaac ccccgcatga
      961 ccaggagcac gtaggccacg atcagcacca gacagccaag gaacccccac atctcggcga
     1021 acacggcaaa aatgaaatca gtatgccgct ccggcaggaa cgagaggcgg ctctgcgtcg
     1081 cgccgcccat ccccttcccc aggagctggc ccgaaccgat ggcgatcttc gcctgaatga
     1141 cattgtaggc cgttccgagc ggatcgcgga acggatcgag atagacgagc agccggtccc
     1201 gctggtaggg cttgagcacg agccaggcga gtggcatcgc ggccaccccc gcgagcgaca
     1261 gcgcgcccag gactttcagc cggatcccga tgcccaccaa tatcgcggcc agcaccggca
     1321 ccaggaccag ggcggtaccc agatccggct ggcgtaccac cagcaggaag ggaaccccca
     1381 gcagcacgaa gatgccgatg agcatccccc gcgagaaccg ttcgccgcgg ccggaggtga
     1441 gcgcccacgc cagcgtgatg atgaagatca gcttgaagag ctccgagggc tgcaccgtga
     1501 acgggccaag gtggatccag cgccgggcgc ccgagacggt gcggcccagg acgaagaccg
     1561 aggtgaggag gcccaggccc agcacgtaga ggaccggggc ggaccgcacc aggttccggt
     1621 agtccacgct ggccaccacc agcaggccca gcagcccgac gccgacccag gagagctgcc
     1681 gccacacgag cgacgagatg ccccggccgg gattgaggct ccacaggcag gtgaggctga
     1741 gcccgatgat gaacagggcg gcgcccagga gcagccaatc gacgttctga agcaggcgcc
     1801 ggtcgagccg gagcatcaac cgggctcggg aagagtcacc tgcgcgacct tctggaggaa
     1861 gagggcctcg tagatcttgc gcgcgatggg ggccgccacg tcgccgccgt ggccgccgcg
     1921 ctcgacgaac accaccacca ccgcctgcgc gtcgtcggcg ggcgcgaagg acgcgaacca
     1981 cgcgtggtcc tgccccttgg ccgagtcgct cttggcgatg gtctgggcgg tgccggtctt
     2041 gcccgcgatg tccaggcccg ggagccgcgc cgccgccccg gtgccctcct tgacgaccgc
     2101 ggacaaggcg ttccggagaa acgcccacac caccggcgag agctccaccc gctccgtcat
     2161 cttgctggtg gcgctgtagg ccagagagcc gtccgccttc gccacgcgct gcacgaggcg
     2221 gggcttccac aggatgcccc cgttggccac cgctcccatc atccgggcga cctggagcgg
     2281 ggtcaccagc agctggccct gcccgatgga catgttcacg gtgtcgccgg actgccacgg
     2341 gcgcttgttg cgcgggcggg cggccgcgaa cggcacgagc ccgaatcgct cgccgcccag
     2401 gtcgatgccg gtcggcgcgc cgaggccgaa ggcctgggcg taccgggcca ttgtgtcgcc
     2461 gccgaccttg aggccggcct ggtagaagta gacgtcgcac gactgggcga cggcggtgcg
     2521 caggtccacg tggccgtgcc cgcccgcctt ccagtccttg aacgtccagt ggcccaggct
     2581 gaactccccg ttgcagtaga tccggtccat cggggcgagc gagccctcct ggagaccagc
     2641 cgcggccacg atgatcttga acaccgagcc gggcgggtac tgcccctgca gggcgcggtt
     2701 catgagcggg gtcatcgggt ccttcaccag acgcagccac tcgtcgcggt cgatgttccc
     2761 ggtgagacga tcgaggggga acgcgggact cgacgtcaga gccagcacgt cgccattacg
     2821 cggatccatg accaccaccg agccggcctt gcccgccatc gcctgctccg ccacttcctg
     2881 gatccggcga tcgatggtgg tgacgacctg ggcccccggg tccggctcct cgcgccgcat
     2941 cacctgcacc ggccgaccca gggcgtcgac ctcgatccgc tcgcccccgt cccgcccgcg
     3001 caggtactcg tccagaagcc gctccaggcc cgactgcccg atcatgtccc cgcggcggta
     3061 ccgcccctgc ttcatctgct cgtcgctcac ctcgcggacg tagccgagca ggtgggccgc
     3121 gaacgtgctg gtgggataca cgcgctgggg ctcgacctcc acgatcacgc cgggcagctc
     3181 gagcttgcgc tcctccacct tggtcacgtc ctcgagcgtg agaccccgcc gcacgcgcac
     3241 cggacggatc gagtcggccg gcacgcgctc cagcgcgtcc atgaggtcgc cgaccggcat
     3301 cttcaggaga acggccaggc gtgccaccac cgagttgcgg tcctccatct ccctcgggat
     3361 cagggagagg gtgaacgcgg ggcggttgtc caccagcgcc aggccgttgc ggtcgaagag
     3421 gatgccgcgg ggcgccgcca ccggccggac ccggatgcgg ttcttctccg acagctcctg
     3481 catgcggccg ccttcgagca cctggaggta ccagagctgg ccgaccaggc cgacgaagcc
     3541 gatcgcggtg accaccgcga gggcgagcac gcggcggccc caggcttcgc gtcgattggc
     3601 cggagacggg ttcagcgcca cgtgcgcctc cgtaccagct cgaagctcgc ctcggccagg
     3661 atgcagaggg taccgatcag cccgttgtag agcgcctgcg gcaggatcac gtggagcatc
     3721 aggtcgccga aggccgccgg atagtggaac atctgcagga gcaggaagcg ccccaacccc
     3781 tcggagatgg tgagaagcac gaggcccggg acctgcacga gcgggttgct gacccagaag
     3841 cgtcccacca gcaggcccat tccgaagccg gccagaccct tggtgagggc ctgcacgccc
     3901 acgagacctc cgccggtcgc gtcctggagc aggcccgcca cgaagccggc caggcaaccc
     3961 gactccggcc cgtaacgcag ggcgatgagc acggtgagga tgagggggat gtcgggggtg
     4021 attcccgcca ctcccgccac cgcgaccacc gtcgactgca gcacgccccc gccgacgatg
     4081 acgagcagca cgaggaggcg gagcacgcgt cacccctccg acgggaagag cgtggccacg
     4141 tcctgggtgg tctgcccggt cacgagcagg acctcctcga cgcgggagaa gtcgaccgcg
     4201 ggcgcgagga ccgcgaagtg gaagagggcc gagcccttgt cctcgatctt gatcacgcgg
     4261 ccaaccggga ggcccttggg gaagagcgtg ccgaggcccg aggtgaccac gaggtccccc
     4321 ggcgcgacgc tcgcgccgtc gcgggccatg aacttgaacc ggacggtgcc gcccgcgtcg
     4381 ccttccacca ggcccgcggt ccgggtgcgc tgcacgaccg cgcccaccgt cgacgccgga
     4441 tcgttgagga gctggatcac cgccgcgccc cggtggacct gaacgacccg gccgaccagg
     4501 ccgtccggca cgatgaccgg ggtctgctgg gcgatgccgt cgccgcgccc ccggttcacg
     4561 gtcagggagc gcacccagcc cccggcctca cgcccgatga cctcgccggc cacggtggcc
     4621 agcggcaggc ggtccttcag caccagcagc cggcgcaggc gggcattctc ctcgcgggtc
     4681 tcgccggcct gcatggcctg gacacggagg tgctcgttct cctcgtggag cgtggcgttg
     4741 tcgcggcgga ccgacttcca gtccacgtag ttggcccaga agttgagggc cccgcggtgg
     4801 atgcggacga ggaggctctg gacgggggtg accgcgatcg tgaccagctc tcccgcccgt
     4861 ccggttcccc ccccgcgcgt ctggatcgtc agcagcagta gggacaccag tagcaccgcg
     4921 agcaggattc cgtatctgcc aactctcacc gctccgtcac ccttacggcc gatcagcgcg
     4981 aggccggcc
//
LOCUS       DATMCV010000539         3276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_227463, whole genome shotgun sequence.
ACCESSION   DATMCV010000539 DATMCV010000000
VERSION     DATMCV010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227463"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2577)
                     /gene="uvrA"
                     /locus_tag="VNU03_12460"
     CDS             complement(<1..2577)
                     /gene="uvrA"
                     /locus_tag="VNU03_12460"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWP75324.1"
                     /translation="MANDRIVIRGAREHNLKGIDVEIPRDQLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLEQMEKPDVDSIEGLSPAISIEQKTTSKNPRSTVGT
                     VTEIYDYLRVLFARVGTPHCPQCGNVISAQTVQQMVDRVMTLPVGSRLVVLAPIIRGR
                     KGEYRKLFFDLRRQGYVRVRVNGQLRELTEDIELAKTKKHTIEVVVDRLIVKDTLGGR
                     LADSLETALRLSDGIVQVEVPDGPAFMFSERLACAACGISFPEVSPRMFSFNNPYGAC
                     PECGGIGTRYEIDPERVVPDQNKTLAGGALLPWAGPTASGIFKQTLRVLAKRHGFTLE
                     TPWAKLPKKTRDVILHGEPDDGFEGVVKLLERRYKETTSEETRAEVEHFMTERACPTC
                     GGSRLRRETLSVKIAGRSIADVVRFPIKAARQFFDTLTLTEREMAIARRVLKEIRERL
                     GFLSQVGLDYLTLDRPAATLSGGEGQRIRLATQIGSSLVGVLYILDEPSIGLHQRDNG
                     RLLDTLKRLRDLGNTVLVVEHDEETMRAADYVVDLGPGAGELGGHLVAVGTPDEVMAN
                     PDSLTGRFLAGVERIDPPAVRRKSNGKFVTIHNPREHNLKGMNVRIPLGTFTAVTGVS
                     GSGKSTLVNDILYRALASMLHRAQDRPGAHDRIEGAQHLDKVIDIDQSPIGRTPRSNP
                     ATYTGVFTLIRTLLARTPDARMRGYQPGRFSFNVKGGRCEACQGDGLVKIEMHFLPDV
                     YVTCEVCRGKRYNRETLEVRYKGKNIADVLDMTVAEALAFFEPVPSIKQKLQTLHDVG
                     LDYIRLGQAATTLSGGEAQRVKLATELSRRATGRTLYILDEPTTGLHFADIRRLLEVL
                     NQL"
     gene            complement(2583..>3276)
                     /locus_tag="VNU03_12465"
     CDS             complement(2583..>3276)
                     /locus_tag="VNU03_12465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75325.1"
                     /translation="ALYARGGIQWCFLGAAAIGMGIGLLILRLRVAAPIASGAPRFRW
                     VSRSALPAAATVIATNVGYSSIYAFLPLYALASGLDGNLGWFYALFSACIILGRLTLS
                     RCSDRIGRARVIVPAIAASGLSYLVLAVPPRPATLAAGAVLLGAGVALFYPTLLALLV
                     DRTPERERGSAIGTLSGSFDLGNVVGSFLVGVTVERVSYAAGFHVAAAGALLGLVLFV
                     LSERRASGQGIM"
BASE COUNT          490 a         1120 c         1185 g          481 t
ORIGIN      
        1 cagctgattc agcacttcca gcagacgccg gatgtccgcg aagtgcagcc cggtggtggg
       61 ctcgtcgagg atgtagagcg tgcggccggt ggcccgccgg ctcagctcgg tggcgagctt
      121 cacgcgctgg gcctcgcccc cggagagcgt ggtggcggcc tgccccagac ggatgtagtc
      181 gagccccacg tcgtgcagcg tctggagctt ctgcttgatc gagggcaccg gctcgaagaa
      241 cgcgagcgcc tccgccaccg tcatgtcgag gacgtcggcg atgttcttgc ccttgtagcg
      301 gacctccagc gtctcgcggt tgtagcgctt gccgcggcag acctcgcagg tcacgtacac
      361 gtcgggcaag aagtgcatct cgatcttcac cagcccgtcg ccctggcagg cctcgcagcg
      421 cccgcccttg acgttgaacg agaagcgccc cggctggtag ccgcgcatgc gcgcgtccgg
      481 cgtgcgcgcg agcagggtgc ggatgagcgt gaagaccccg gtgtaggtgg ccgggttcga
      541 ccgcggcgtc cgaccgatcg gcgactggtc gatatcgatc accttgtcga ggtgctgcgc
      601 gccctcgatg cggtcgtggg cacccggccg gtcctgcgcg cggtggagca tcgaggcgag
      661 cgcgcggtac aggatgtcgt tgaccagggt ggacttgccg gagccggaga cgccggtcac
      721 cgcggtgaac gtcccgagcg ggatccggac gttcatcccc ttcaggttgt gctcgcgggg
      781 gttgtggatc gtcacgaact tgccgttgga cttccgccgg accgcgggcg ggtcgatgcg
      841 ctccaccccg gccaggaagc gcccggtcag cgagtccggg ttcgccatca cctcgtccgg
      901 cgtccccacc gccaccaggt gcccgcccag ctctcccgcc cccggaccca ggtccaccac
      961 gtagtcggcg gcccgcatcg tctcctcgtc gtgctcgacc accagcaccg tgttgccgag
     1021 atcgcgcagg cgcttgaggg tgtcgaggag ccggccgttg tcccgctgat ggaggccgat
     1081 cgacggctcg tccaggatgt agagcacccc gaccaggctc gagccgatct gggtggcgag
     1141 acggatccgc tggccctccc cgcccgacag ggtcgcggcg ggccggtcga gggtcaggta
     1201 gtcgagcccg acctgcgaga ggaagccgag gcgctcgcgg atctccttca gcacgcggcg
     1261 ggcgatcgcc atctcgcgct cggtgagggt gagcgtgtcg aagaactggc gcgccgcctt
     1321 gatcgggaag cgcaccacgt ccgcgatgga gcggcccgcg atcttgaccg acagcgtctc
     1381 ccggcggagc cgcgagccgc cgcaggtcgg gcaggcccgc tcggtcatga agtgctcgac
     1441 ctccgcgcgg gtctcctccg aggtggtctc cttgtagcgc cgctcgagca gcttcaccac
     1501 gccctcgaag ccgtcgtccg gctcgccgtg caggatcacg tcgcgggtct tcttcggtag
     1561 cttggcccac ggggtctcga gggtgaaccc gtggcgcttg gcgagcaccc ggagggtctg
     1621 cttgaagatg ccggaggcgg tcggcccggc ccagggcagg agcgcgccgc cggcgagggt
     1681 cttgttctgg tccggcacca cccgctcggg atcgatctcg tagcgggtgc cgatgccccc
     1741 gcactccgga cacgccccgt acgggttgtt gaacgagaac attcgcggcg agacctccgg
     1801 gaacgagatg ccgcacgccg cgcaggcgag ccgctccgag aacatgaacg cgggcccgtc
     1861 gggcacctcc acctgcacga tcccgtcgct caggcggagc gcggtctcga gggagtcggc
     1921 gagccgcccc ccgagggtgt ccttcacgat caggcggtcg accaccacct cgatggtgtg
     1981 cttcttggtc ttcgccagct cgatgtcctc ggtcagctcg cgcagctggc cgttgacccg
     2041 cacccgcacg tagccctgac ggcgcaggtc gaagaagagc ttgcggtact cgcccttccg
     2101 cccgcggatg atcggggcca gcaccaccag gcggctgccc accggcaggg tcatcacccg
     2161 gtccaccatc tgctgcacgg tctgggccga gatgacgttg ccgcactggg ggcagtgggg
     2221 cgtgcccacc cgcgcgaaga gcacccggag gtagtcgtag atctcggtga cggtgcccac
     2281 cgtggagcgc gggttcttcg aggtggtctt ctgctcgatg gagatcgcgg gggagagccc
     2341 ctcgatcgag tcgacgtcgg gcttctccat ctgctcgagg aactggcgcg cgtaggcgga
     2401 gagcgattcg acgtagcggc gctggccctc cgcgtagatg gtgtcgaagg cgagggagga
     2461 cttgcccgac ccggacaggc cggtgatgac gacgagctgg tcacggggga tctcgacgtc
     2521 gatgcccttg agattgtgct cgcgggcccc gcggatgacg atgcggtcgt tggccatgga
     2581 cgctacataa taccctgccc gctcgcgcgc cgctcgctca gcacgaacaa caccaggccg
     2641 agcagcgcgc cggccgcggc gacgtggaag ccggccgcgt aggacacgcg ctcgacggtg
     2701 acgccgacca ggaaggagcc gaccacgttg ccgagatcga aggagccgga gagcgtgccg
     2761 atcgccgagc cgcgttcgcg ctccggcgtg cggtcgacca ggagggcgag cagcgtgggg
     2821 tagaagagcg ccacccccgc gcccagcagg accgcgcccg cggccagcgt ggccgggcgc
     2881 ggcggcaccg ccagcacgag gtagctcagc ccggaggccg cgatcgcggg cacgatcacg
     2941 cgcgcgcgtc cgattcgatc ggagcagcgg ctcagggtca gccgcccgag gatgatgcag
     3001 gccgagaaca gggcgtagaa ccagcccagg ttgccgtcga ggccggaggc gagcgcgtac
     3061 agcgggagga acgcgtagat cgagctgtag cccacgttcg tcgcgatcac ggtggccgcc
     3121 gcgggcagcg cgctccggct gacccagcgg aagcgcggcg cgcccgaagc gatcggtgcg
     3181 gcgacccgca ggcgcaggat gagcaggccg atgcccatcc cgatcgcggc cgcgcccagg
     3241 aagcaccatt gtatgccgcc gcgcgcgtag agcgcg
//
LOCUS       DATMCV010000540         2712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_496003, whole genome shotgun sequence.
ACCESSION   DATMCV010000540 DATMCV010000000
VERSION     DATMCV010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496003"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VNU03_12470"
     CDS             complement(<1..915)
                     /locus_tag="VNU03_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWP75326.1"
                     /translation="MCGIFGYVGGGRGSADAAGIVLRGLKKLEYRGYDSWGVAVAHDG
                     GVRLEKHVGKIGEASVALPPSSLGVGHTRWATHGGVTEPNAHPHLDCRGRLALIHNGI
                     VSNYRELRDTLDLSSHRLRSETDTEVIVHLLEDRLARTPEGPEHLVTALVAVFRQLQG
                     LNAVAVLDVRTSQLAVAKSGSPIVLGWGDGESFLASDYSALLEHTRRVTFLEDQQAAL
                     VEADGIRVFDVATGIEIAPAVSEVEWETAAVELSGYPDFLSKEIHEQPAVLRRLAAGW
                     GGHVRALAGMLREARDVFAVGCGTASHAA"
     gene            complement(1274..>2712)
                     /locus_tag="VNU03_12475"
     CDS             complement(1274..>2712)
                     /locus_tag="VNU03_12475"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP75327.1"
                     /translation="SMDYSYSFSDELQQEWRWSERYSAQPEILRYANHVADRFDLRRD
                     IQFEARVTSAVFDAAAGGWTVETDQADRVTARFCIMATGCLSDAQVPDITGLESFAGK
                     WYHTGKWPHEGVDFTGRRVGVIGTGSSAIQSIPIIAKQASHLYVFQRTPNFSMPAQNT
                     PLDPEYERRVKAGYADFRRQARESRVGFVTERSGDSALAVPAEEREREYEKRWRRGGL
                     GFSAAYTDLLVSKEANDTAARFFREKIRAIVRDAAVADLLCPQSYPLGTKRLCVDTGY
                     YDTFNRDNVTLVGVRKTPIEAITPKGVRVAGVEYAVDDLVFATGFDAMTGALLNIDIC
                     GRGGRKLDTEWAGGPRTYLGLTVEGFPNMFMITGPGSPSVLSNMIVSIEQHVDWIADC
                     VTHLRARGLSTIEATAEAQEAWVQHVNEVGHMTLYPLAPSWYMGANIPGKPRVFMPYI
                     GGVGVYRQKCDEVAAKGYEGFALGKSRV"
BASE COUNT          383 a          968 c          912 g          449 t
ORIGIN      
        1 cgcggcgtgg ctcgcggtgc cgcagcccac cgcgaagacg tcacgcgcct cgcgcagcat
       61 gccggcgagc gcgcggacgt gcccgcccca cccggcggcg aggcgccgga gcaccgccgg
      121 ctgctcgtga atctccttgc tcaggaaatc cgggtagccg gagagctcga ccgccgctgt
      181 ctcccactcg acctcgctga ccgcgggtgc gatctcgata ccggtggcca cgtcgaacac
      241 ccggatcccg tcggcctcga ccagcgccgc ctgctggtct tccaggaagg tgacccggcg
      301 cgtgtgctcc agcagcgcgc tgtagtcgga ggccaggaag ctctcgccgt caccccagcc
      361 gagcacgatc ggcgagccgc tcttggcgac cgcgagctgg ctggttcgca cgtccagcac
      421 cgctaccgcg ttcagccctt gcagctggcg aaagaccgcc acgagggcgg tcacgagatg
      481 ctcgggtcct tcgggggtgc gcgcgagccg gtcctccagc aggtgcacga tcacctcggt
      541 gtcggtctcg gagcgcaggc ggtggctcga gaggtcgagc gtgtctcgga gctcccggta
      601 gttcgagacg atgccgttgt ggatgagggc caggcggccg cggcagtcga ggtgagggtg
      661 cgcgttgggc tccgtcaccc cgccgtgggt ggcccagcgg gtgtgcccga cgccgaggga
      721 gctcggcggc agggccacgc tggcctcgcc gatcttgccg acgtgcttct ccagtcgcac
      781 gccgccgtcg tgtgccaccg cgacccccca ggagtcgtag ccccgatact cgagcttctt
      841 caggccgcgc aggacgatgc ccgccgcgtc cgcgcttccc ctcccgccgc ccacatagcc
      901 aaaaattcca cacatggtcg ctcccctttc tccgaggatg gagaggcgcg ggtcgacaaa
      961 ccgcgaacgt cgagagtgac gacggggaag agcaggggat ttcaggtcgc gggtcgttgc
     1021 gacccgccgg cacgctgggt ggtctttgtc agcgcgtcac cgcgctggtt tgctccaccc
     1081 atcacgactg tgccaaccga ggggccatcc gccgaatgct tcgatcagcc cctacctcgt
     1141 catccgcccg aaggcggacc tggcgctctt tacgcttgct ccctggctat cccttcctgg
     1201 tctttccttc ccggctcgca gtcagctcgc tctcgcgtcc ctcctccgga ttggatgatg
     1261 tccgctgatc ctatcacacc cgggatttgc cgagcgcgaa gccctcgtag cccttcgccg
     1321 ccacctcgtc gcacttctgc cggtagacgc cgaccccgcc gatgtagggc atgaacaccc
     1381 gcggcttgcc cgggatgttg gcccccatgt accacgaggg cgcgagcggg tagagggtca
     1441 tgtggccgac ctcgttcacg tgctggaccc acgcctcctg ggcctccgcg gtcgcctcga
     1501 tggtcgacag gccgcgcgcg cgcaggtggg tgacgcagtc ggcgatccag tccacgtgct
     1561 gctcgatgga cacgatcatg ttgctcagca ccgacgggct gccggggccg gtgatcatga
     1621 acatgttcgg gaagccctcg acggtcaggc cgagataggt gcgcggaccg cccgcccatt
     1681 ccgtgtcgag cttgcggccg ccgcgcccgc agatgtcgat gttgagcagc gcgccggtca
     1741 tcgcgtcgaa gccggtggcg aagacgaggt cgtccaccgc gtactcgacg ccggccacgc
     1801 gcacgccctt cggggtgatg gcctcgatcg gggtcttgcg gacgcccacc agcgtcacgt
     1861 tgtcgcggtt gaacgtgtcg tagtagccgg tgtccacgca gagccgcttg gtgcccaggg
     1921 ggtagctctg cgggcacagc aggtccgcca ccgccgcgtc acgcacgatc gcccggatct
     1981 tctcgcggaa gaaccgggcc gcggtgtcgt tggcctcctt gctgacgagc aggtcggtgt
     2041 acgcggccga gaacccgagg ccgccacggc gccagcgctt ctcgtactcg cgctcccgct
     2101 cctcggccgg caccgccagc gcggagtcgc cgctccgctc ggtcacgaag cccacgcggg
     2161 actcacgggc ctggcgccga aagtcggcgt agcccgcctt cacgcggcgc tcgtactccg
     2221 ggtcgagcgg ggtgttctgg gcgggcatgc tgaagttggg cgtgcgctgg aagacgtaga
     2281 ggtggctcgc ctgcttcgcg atgatcggaa tcgactggat cgcggaggag ccggtgccga
     2341 tcacgccgac ccgccggccg gtgaagtcca cgccctcgtg cggccacttc ccggtgtggt
     2401 accacttgcc cgcgaagctc tcgagccctg tgatgtcggg cacctgcgcg tccgagaggc
     2461 acccggtcgc catgatgcag aaccgtgcgg tcacccggtc ggcctgatcg gtctcgaccg
     2521 tccagccgcc cgccgccgcg tcgaacaccg cggaggtcac acgcgcctcg aactggatgt
     2581 cgcgccgcag gtcgaagcgg tccgccacat ggttggcgta tctcaggatc tccggctggg
     2641 ccgagtagcg ctcgctccag cgccactcct gctgcagctc gtccgagaac gagtacgagt
     2701 agtccatgct ct
//
LOCUS       DATMCV010000541         6315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_897040, whole genome shotgun sequence.
ACCESSION   DATMCV010000541 DATMCV010000000
VERSION     DATMCV010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897040"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VNU03_12480"
     CDS             <1..806
                     /locus_tag="VNU03_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWP75328.1"
                     /translation="EMWGLTYAPSGEKARQYTVTHAPLATADDLARLPVADPGSGALG
                     EQLDALRLIRAGVGPDVPIIWTVFAPPMILPMLARGGREQALGFLRDAPRETARAFDV
                     MADTLAEYARRCLAAGADGLFYATNVATRALMSAEECRRWQRPWDLRILEAVEGAPFN
                     LLHVCGAGIHLDEFADYPVTALSFATVPGNPTLAAAHGKTGRAVVGGLPAKPEIAGLT
                     EAALAERTASARREMKDRWLLLGPDCSINPDTPERLLHAVGAAARQAAP"
     gene            875..2950
                     /gene="fusA"
                     /locus_tag="VNU03_12485"
     CDS             875..2950
                     /gene="fusA"
                     /locus_tag="VNU03_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWP75329.1"
                     /translation="MALDIGKIRNVAFVGHGGVGKTSLVEALLHACGATTRLGKVDDG
                     TTTTDFDPDEIKRKISLNTAAAFCDHKGHRLNLIDTPGYGDFVADARAGLRVASAAIV
                     VVDAVAGVQVQTEKVWKFANEYNLPRAIVINRLDRERADFYRTLEALQKRLKGRLVPL
                     QLPIGSEAGFGGVVDLITMRALAHADGRAKDAEIPADLEESARSYREKLSEAAAETDD
                     DLLAKYLEEGAIGEEEMLGALRRAIMSGGVVPVLCASATRGIGVGSLLDLIVKEFPSP
                     ADQGEVEGADPRTKATVKRAPDAKSPLCAIVFKTITDPHVGKLSVFRVYSGTLRADSQ
                     VYNASRDSRERVGHLGWLSGKTQKAVDALGPGEIGVVAKLKDTLTGDTLCDEGSPVGL
                     PGISFPEPAISFAIQPKTRGDEDKISTALHRMAEEDPTLHHHYDPETKQLLVSGMGQL
                     HVEVVVERMKRKYNVDVSLLPPRIPYKETVKGRAEVQGKYKKQTGGRGQYGDTWLRIE
                     PLTRGGGFEFVDDIFGGAIPRNFIPSVEKGVRECMKRGIYAGYPVVDLKVTLYDGSYH
                     DVDSSDMAFQIAASMGLQKGFMDAKPCLLEPVMHVEVNVPSEGAGDVIGDLNGRRGRI
                     VGMEPEGDVVAVRAQVPMSEMLTYESSLRSMTGGRGGYSMEFSHYEEVPSQLAEKVVA
                     AHRAEKEKH"
     gene            3026..4261
                     /locus_tag="VNU03_12490"
     CDS             3026..4261
                     /locus_tag="VNU03_12490"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWP75330.1"
                     /translation="MFVTHIECTVCGQRHEAGRLLTVCQACGQMLAVRYDLPRVAAAV
                     SKETLQSRPPGMYRFRELLPLAAGEEPVTLGEGGTPLLELPRLAAHLGLRHLWAKDEG
                     QNPTGTFKARGLGMAITKARTLGVRGLMIPSAGNAGGAAAVYGARAGLPVAVVVPRDT
                     PEAAIAEAALAGAHVFTIDGTIATAGRAIASVAPALGWFDLATLKEPYRLEGKKTMGL
                     ELAEQLGWDAPDVLVYPTGGGTGLVGIWKAYEELGTMGWVKAGQPRFIAVQAEGCAPL
                     VRAWTDKAETTSVVENPMTDAPGLRVPGPFAGRQMLRILRETDGFPQPVSEREIVDAQ
                     RLLARVEGIWTAPEAAATLAALLHMKADGQVEPTTRVVLVLTGAGIKYPPPALPRPAH
                     LEGTPEAMLARFKEAIGQP"
     gene            4258..5523
                     /locus_tag="VNU03_12495"
     CDS             4258..5523
                     /locus_tag="VNU03_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HWP75331.1"
                     /translation="MSALTRRGFLRTSGLGLGLLAGGGLAGCAASMKGDFMPKAGPRV
                     VVIGGGWGGATAAKYVRLLDPGVEVVLIEPNRQFVSCPFSNLVLAGLRSIDSLTMGYD
                     GLRKHGVRIIHETASAIEPDTKRVRLGEGYLQYDRLVVSPGVDFQWEQVEGLAQSQDT
                     VLHAWKAGPQTVQLAQQLAAMPDGGVFVLSIPLAAYRCPPGPYERTSMVAWYLKANKP
                     RSKLIVLDANQNIISKAALFRAAWQAYPNIDYRPASRVIGVDPGAREVRTEFDRIRYD
                     VVNIIPPQRAGAIAVQADLVGGDKRWCEVNHVTYESVKQPGIHVIGDATIGLPVPKSG
                     NVANAMGKIAAASVVSLIRGQQPTALAPGNTCYSWVSDREAIAVVNAYKIENGKVVQI
                     EQKLTPAQSPLVAQRAVGWAQSIWADVLA"
     gene            5559..6179
                     /locus_tag="VNU03_12500"
     CDS             5559..6179
                     /locus_tag="VNU03_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWP75332.1"
                     /translation="MPTVLGCLVIAAGLLLGFLSGILLPVTAEAAGPLDDRGALKSIT
                     CAACHGAGGNSRSSAMPIIAGLDAAYFRKQIEDYAAGRRLSAEMEPYAKQVRDLGVDD
                     VAAYFASRRREPTPVPSPADAIERGSRAAAQCVVCHGPRGQGDAAKGIPNLAGQAPGY
                     LREQMLLFKQDRRSPGDPALAALKALLKTVPDETFGDLAAYYSSLR"
BASE COUNT          936 a         2279 c         2257 g          843 t
ORIGIN      
        1 ccgagatgtg gggcctcacc tacgccccgt cgggggagaa ggcgcggcag tacacggtca
       61 cccacgcgcc gctcgccacc gccgacgacc tcgcgcggct cccggtggcg gatcccggct
      121 cgggcgccct cggggagcag ctcgatgccc tccggctgat ccgggccggg gtcggccccg
      181 acgtgccgat catctggacg gtgttcgcgc cgcccatgat cctgccgatg ctggcgcgcg
      241 gcggccgcga gcaggccctg ggcttcctcc gcgacgcgcc ccgcgagacc gcgcgcgcct
      301 tcgacgtgat ggcggacacc ctcgccgagt acgcgcggcg ctgcctggcc gccggcgccg
      361 acggcctctt ctacgcgacc aacgtggcga cgcgcgcgct catgagcgcg gaggagtgtc
      421 gccgctggca gcggccgtgg gacctgcgga tcctcgaggc ggtggaaggc gctccgttca
      481 atctgctcca cgtctgcggg gcgggcatcc acctggacga gttcgcggac tacccggtca
      541 ccgcgctctc gttcgccacc gtgccgggca acccgacgct cgcggccgcg cacggaaaga
      601 ccgggcgcgc ggtggtgggc gggctgccgg ccaagcccga gatcgcgggc ctgaccgagg
      661 ccgcgctcgc cgagcgtacc gcctcggcgc ggcgggagat gaaggaccgc tggctcctcc
      721 tcggccccga ctgctccatc aatccggaca ccccggagcg gctcctccac gcggttggcg
      781 ccgcggcgcg gcaggccgcg ccctaggggg ctggaagggc ctgtaacgct gtggtagcat
      841 ccggcccaca ccttctcctt tccaggaggc ggacatggcg ctcgacatcg gcaagatccg
      901 caacgttgcg ttcgtcggcc atggcggggt gggcaagacg tccctcgtcg aagctctcct
      961 gcacgcctgc ggagccacca cgcggctcgg caaggtcgac gacggcacga ccaccaccga
     1021 cttcgacccc gacgagatca agcgcaagat ctcgctcaac accgcggcgg ccttctgcga
     1081 ccacaagggc catcgcctca acctgatcga cacccccggg tacggcgact tcgtggccga
     1141 cgcccgggcg gggctgcggg tggccagcgc ggcgatcgtg gtggtggacg cggtggcggg
     1201 cgtgcaggtg cagaccgaga aggtctggaa gttcgccaac gagtacaacc tgccccgcgc
     1261 gatcgtgatc aaccggctcg accgcgagcg ggccgacttc taccgcacgc tcgaggcgct
     1321 gcagaagcgc ctgaagggcc ggctcgtccc gctccagctg ccgatcggca gcgaggccgg
     1381 cttcggcggg gtggtggacc tcatcaccat gcgcgccctc gcccacgcgg acggccgggc
     1441 gaaagacgcg gagatcccgg ccgacctgga ggagagcgcc cgatcctacc gggagaagct
     1501 cagcgaggcc gcggccgaga ccgacgacga cctgctcgcc aagtacctgg aggagggcgc
     1561 catcggcgag gaggagatgc tcggcgccct gcgccgcgcg atcatgtccg gcggggtggt
     1621 gccggtgctc tgtgcctccg cgacgcgcgg catcggggtg gggtcgctcc tcgacctcat
     1681 cgtgaaggag ttcccgtcgc ccgccgatca gggtgaggtg gagggcgcgg acccgcggac
     1741 caaggcgacc gtcaagcgcg cccccgacgc gaagtcgccc ctctgcgcga tcgtgttcaa
     1801 gaccatcacc gacccgcacg tgggcaagct ctccgtcttc cgggtctact cgggcacgct
     1861 gcgggcggac agccaggtct acaacgcgag ccgcgacagc cgcgagcggg tggggcacct
     1921 gggctggctc tccggcaaga cccagaaggc ggtcgacgcg ctcgggcccg gcgagatcgg
     1981 ggtggtggcc aagctcaagg acacgctcac cggcgacacc ctctgcgacg agggcagccc
     2041 ggtcgggctg cccggcatca gcttcccgga gccggccatc tccttcgcga tccagccgaa
     2101 gacccgcggc gacgaggaca agatctcgac cgcgctccac cggatggccg aggaggatcc
     2161 gaccctgcac caccactacg atcccgagac caagcagctg ctggtctccg ggatgggcca
     2221 gctccacgtc gaggtggtgg tggagcggat gaagcgcaag tacaacgtgg acgtctcgct
     2281 gctgccgccg cgcatcccct acaaggagac ggtcaaggga cgcgccgagg tgcagggcaa
     2341 gtacaagaag cagaccggcg ggcgcgggca gtacggcgac acctggctgc gcatcgagcc
     2401 cctcacgcgc ggcggaggct tcgagttcgt cgacgacatc ttcggaggcg ccatcccgcg
     2461 caacttcatc ccgtcggtgg agaagggcgt gcgcgagtgc atgaagcgcg ggatctacgc
     2521 gggctacccg gtggtggacc tcaaggtcac cctctacgac ggctcctacc acgacgtcga
     2581 ctcctcggac atggccttcc agatcgccgc ctccatgggc ctgcagaagg gcttcatgga
     2641 cgccaagccg tgcctgctcg agccggtcat gcacgtcgag gtgaacgtgc cgtcggaggg
     2701 cgcgggcgac gtgatcggcg acctgaacgg ccgacgcggc cgcatcgtgg gcatggagcc
     2761 ggagggcgac gtggtggcgg tgcgcgccca ggtgcccatg tccgagatgc tcacctacga
     2821 gtcgagcctg cgctccatga ccggcggtcg cggcggctac tcgatggagt tctcgcacta
     2881 cgaggaagtc ccgagccagc tcgccgagaa ggtggtggcc gcccaccgcg ccgagaagga
     2941 aaagcactag cggatcgcgg cgcgcccatc ccgggcgcgc cgcgccgcct ccgatgcccc
     3001 acgttccaac gaccgaggcc cacgcatgtt cgtgacccac atcgaatgca ccgtctgcgg
     3061 ccagcgtcac gaggccgggc ggctcctcac cgtttgccag gcctgcggcc agatgctcgc
     3121 ggtccgctac gacctgcccc gcgtcgcggc cgccgtctcc aaggagacgc tccagagccg
     3181 gccgcccggg atgtaccgat tccgcgagct gctgccgctg gccgcgggcg aggagccggt
     3241 gacgctcggc gagggcggca ccccgctcct cgagctgccg cgcctggccg cgcacctcgg
     3301 gctccggcat ctctgggcca aggacgaggg ccagaacccg acgggcacct tcaaggcgcg
     3361 cgggctcggg atggccatca ccaaggcgcg caccctcggc gtccgcgggc tcatgatccc
     3421 gtccgcgggc aacgcgggcg gcgccgccgc ggtctacggg gcgcgggcgg gtctcccggt
     3481 cgcggtggtc gtgccgcgcg acacgcccga ggccgcgatc gcggaggccg cgctcgccgg
     3541 ggcgcacgtc ttcacgatcg acggcaccat cgccaccgcc gggcgggcca tcgcgtcggt
     3601 cgcgcccgcg ctcggctggt tcgacctggc cacgctcaag gagccctatc ggctcgaggg
     3661 caagaagacg atgggtctcg agctggccga gcagctcggc tgggacgccc ccgacgtgct
     3721 ggtctacccg accggcggcg gcaccgggct ggtcggcatc tggaaggcct acgaggagct
     3781 cggcaccatg ggctgggtca aggccggcca gccgcgcttc atcgcggtgc aggccgaggg
     3841 ctgcgccccg ctcgtgcgcg cctggacgga caaggccgag accacctcgg tcgtggagaa
     3901 cccgatgacc gacgcccccg gcctccgcgt gcccggcccc ttcgccgggc ggcagatgct
     3961 ccgcatcctc cgcgagaccg acggcttccc gcagccggtg agcgagcggg agatcgtcga
     4021 cgcccagcgg ctcctcgcgc gcgtcgaggg gatctggacc gctcccgagg cggccgcgac
     4081 cctggccgcg ctccttcaca tgaaggccga cgggcaggtc gagcccacca cgcgcgtcgt
     4141 gctcgtcctc accggcgccg gcatcaagta cccgccgccc gcgctgccgc ggccggcgca
     4201 tctcgaggga accccggagg cgatgctcgc ccggttcaag gaggcgatcg gacagccatg
     4261 agcgcactca ctcgcagggg gttcctccgg acgtcgggcc tcggcctggg cctgctggcc
     4321 ggcggtggcc tggccggctg tgccgcgtcg atgaagggcg acttcatgcc gaaggccgga
     4381 cctcgggtcg tcgtcatcgg gggcgggtgg ggcggcgcca ccgcggcaaa gtacgtgcgg
     4441 ctgctcgacc cgggcgtcga ggtcgtcctg atcgagccca accgccagtt cgtctcctgc
     4501 ccgttcagca acctggtcct ggccggtctc cgcagcatcg actccctcac catgggctac
     4561 gacggcctcc gcaagcacgg ggtgcggatc atccacgaga ccgcgagcgc catcgagccc
     4621 gacaccaagc gggtgcgcct cggcgagggc tatctccagt acgaccggct ggtggtctcg
     4681 cccggggtgg acttccagtg ggagcaggtg gaggggctcg cgcagagcca ggacacggtc
     4741 cttcacgcgt ggaaggcggg gccgcagacg gtgcagctcg cgcagcagct ggccgcgatg
     4801 ccggacggcg gggtgttcgt gctgagcatc cccctcgcgg cctatcgctg cccacccggg
     4861 ccctacgagc ggacctcgat ggtggcctgg tacctcaagg ccaacaagcc ccggtccaag
     4921 ctgatcgtgc tggacgccaa ccagaacatc atctcgaagg ccgcgctctt ccgcgccgcg
     4981 tggcaggcct accccaacat cgactaccgg ccggccagcc gcgtgatcgg ggtggatccg
     5041 ggggcgcgcg aggtgcggac cgagttcgac cggatccgct acgacgtggt caacatcatc
     5101 ccgccccagc gcgcaggcgc catcgcggtg caggcggacc tggtcggcgg cgacaagcgc
     5161 tggtgcgagg tcaaccacgt gacctacgag tcggtgaagc agccgggcat ccacgtgatc
     5221 ggcgacgcca ccatcggcct gccggtgccg aagtcgggca acgtggccaa cgcgatgggc
     5281 aagatcgccg cggcgtcggt ggtgagcctg atccgcggcc agcagccgac cgcgctggcg
     5341 cccggcaaca cctgctacag ctgggtgagc gatcgcgagg ccatcgcggt ggtcaacgcc
     5401 tacaagatcg agaacggcaa ggtcgtgcag atcgagcaga agctcacgcc ggcccagagt
     5461 ccgctggtcg cccagcgcgc ggtgggctgg gcccagtcca tctgggcgga tgtcctcgca
     5521 tgagcgccgc cggagtcggg tccagaagga gggcttgcat gccgaccgtg ctcggttgcc
     5581 tcgtcatcgc cgccgggctc ctgctcggct tcctgtccgg catccttttg ccggtgaccg
     5641 cggaggcggc cggcccgctc gacgaccgcg gcgcgctcaa gtcgatcacc tgcgcggcgt
     5701 gccacggcgc gggcgggaac agccggtcga gcgccatgcc gatcatcgcc gggctcgacg
     5761 cggcctactt caggaagcag atcgaggact acgcggccgg ccggcgcctg tcggcggaga
     5821 tggagcccta cgccaagcag gtgcgcgacc tgggcgtgga cgacgtcgcg gcctacttcg
     5881 cctcgcgccg gcgcgagccc accccggtgc cgtcgcccgc ggacgcgatc gagcgcggaa
     5941 gccgcgcggc cgcccagtgc gtggtctgcc acgggccacg cggccagggc gacgcggcca
     6001 agggcatccc gaacctggcc ggccaggccc ccggctacct gcgcgagcag atgctcctgt
     6061 tcaagcagga ccggcgcagt ccgggcgatc cggcgctggc ggcgctgaaa gcgctgctga
     6121 agacggtccc ggacgagacc tttggcgatc tcgccgcgta ctactcgagt ctccgctagc
     6181 gcgactcgcg ctatattggt ggctcccatg agccaccttg gaggaggaac ggcggggtcg
     6241 cctgcgtgac gctggccctc gctctccttc tcttcggcct cgcgttctgc ggggccttca
     6301 tctccgggct ggtgg
//
LOCUS       DATMCV010000542         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_629345, whole genome shotgun sequence.
ACCESSION   DATMCV010000542 DATMCV010000000
VERSION     DATMCV010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629345"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1393)
                     /locus_tag="VNU03_12505"
     CDS             complement(152..1393)
                     /locus_tag="VNU03_12505"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HWP75333.1"
                     /translation="MPRINEAFLNLKASYLFAEVKRRTQAHRDAHPGVRLIDLGIGDV
                     TLPLPEVVVQALVEATREQARAETLKGYGPYVGYEFLRAAIAAHDFGARGVKLGADEI
                     FVSDGGKSDAANIQELFATDAVVALMDPAYPVYADANVMAGRSGPADASGRYPGFVYL
                     PCTAENDFQPELPDRHVDLIYLCYPNNPTGAVMTRAALRRWVDYARAQEAVIVYDAAY
                     EAYIRDPELPHSIYEVEGAREVAIESRSFSKNAGFTGLRMAYTVVPREVQGRTGAGDS
                     VSLNQLWLRRQSTKSNGPPYVVQRAAAAVYTPEGQKQVRGLIDQYMENARVLREGLQR
                     AGLTVYGGRNAPYIWIRTPAGVSSWDFFDRLLAEANVVGVPGSGFGPSGEGYFRVTAF
                     GSLADSAEAVERIASRLRSSR"
     gene            1529..>2080
                     /locus_tag="VNU03_12510"
     CDS             1529..>2080
                     /locus_tag="VNU03_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75334.1"
                     /translation="MATETTGRVVDADGHVLEPADTWLRYIDPAYRDRAIRIGTDPEG
                     YEVLLIDGRSLKTLRGQLGSLGGIEMDTERLLTRGQVTYAEGSPAGGYDPHARLAVMD
                     AEGIDSVLLYPTIGICWEGHVTDPGLATAYTRAYNRWLVEFCQADPKRLHPVAHISLL
                     DAEGAVEETLRARAEGCVGVFLSP"
BASE COUNT          289 a          753 c          731 g          307 t
ORIGIN      
        1 ccaccgtctt cgcctggatc gtggaccgct ggggctgggg gaccgcgcgc acgctgctcc
       61 tggtgagctg ctccgccgca tggctcgcca tggagctgat gtcacggtgg tacgagcgcc
      121 agcgggtggt gagaccggtg ccggcggtca gctagcggct actccggagc cggctcgcga
      181 tccgctcgac cgcctcggcg ctgtcggcga gggagccgaa cgcggtcacg cggaagtagc
      241 cttccccgct cgggccgaag cccgagcccg ggacaccgac cacgttggcc tcggccagga
      301 ggcggtcgaa gaagtcccac gacgagacgc ccgccggcgt acggatccag atgtagggcg
      361 cgttgcgtcc gccgtacacg gtgagcccgg cccgctgcag gccctcacgg agaacgcgcg
      421 cgttctccat gtactgatcg atcagcccgc gcacctgctt ctggccctcc ggcgtgtaga
      481 ccgccgcggc tgcccgctgg accacgtacg gaggcccgtt ggacttggtc gactgacgcc
      541 tcagccagag ctggttcagg ctcaccgagt ccccggcccc ggtccgcccc tgcacctcgc
      601 gcggcaccac ggtgtaggcc atgcgcaggc cggtgaaccc ggcgttcttc gagaagctgc
      661 gcgactcgat ggccacctcg cgcgccccct cgacttcgta gatcgagtgc ggaagctcgg
      721 gatcccggat gtacgcctcg taggcggcgt cgtagacgat caccgcctcc tgggcgcgcg
      781 cgtagtcgac ccagcgccgg agcgccgcgc gcgtcatcac cgcgccggtc gggttgttcg
      841 ggtagcacag gtagatgaga tcgacgtgcc ggtcgggcag ctccggctgg aagtcgttct
      901 cggcggtgca cggcaggtac acgaagcccg ggtagcggcc gctcgcgtcc gcgggaccgg
      961 agcgccccgc catcacgttg gcgtcggcgt agaccggata ggcggggtcc atcagggcca
     1021 ccaccgcgtc cgtcgcgaac agctcctgga tgttggccgc gtcgctcttg ccgccgtcgc
     1081 tgacgaagat ctcgtccgcg ccgagcttca cgccgcgggc gccgaagtcg tgcgccgcga
     1141 tcgccgcgcg gaggaactcg tagcccacgt agggtccgta gcccttgagc gtctcggccc
     1201 gcgcctgctc gcgcgtggcc tccacgagcg cctggaccac cacctccggg agcggcaggg
     1261 tgacgtcgcc gatgccgagg tcgatcaagc ggacgccggg gtgcgcgtcg cgatgcgcct
     1321 gcgtccggcg cttcacctcg gcgaagagat agctcgcctt caggttcagg aacgcctcgt
     1381 tgatcctggg cacgtcccgc ctcctcgctc cctgcgtgcg cgcgattcta ttcgatcgca
     1441 gcgcgcggcg ccacccgccg cgcggccggc gctgtgcctt gacgccgccg cggcggccgt
     1501 gcagtatcgc gccgaaggga gcgcgcccat ggccacggaa acaacgggac gagtcgtcga
     1561 cgccgacgga cacgtgctgg agcccgccga cacctggctg cggtacatcg acccggccta
     1621 tcgggaccgg gccatccgga tcgggaccga tcccgagggc tacgaggtcc tgctgatcga
     1681 cggccggtct ctcaagaccc tgcgcgggca gctcggctcg ctcggcggca tcgagatgga
     1741 tacggagcgc ctgctcaccc ggggccaggt cacctacgcg gagggcagcc cggccggcgg
     1801 ctacgatccg cacgcgcggc tcgcggtgat ggacgccgag gggatcgaca gcgtcctgct
     1861 ctacccgacc atcggcatct gctgggaggg acacgtcacc gaccccgggc tggccaccgc
     1921 ctacacgcgg gcctacaacc gatggctggt cgagttctgc caggccgatc cgaagcgcct
     1981 gcacccggtg gcgcacatct ccctgctgga cgccgagggt gccgtcgagg agacgcttcg
     2041 cgcccgcgcg gagggttgcg tgggcgtctt cctctcgccg
//
LOCUS       DATMCV010000543         2544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_431334, whole genome shotgun sequence.
ACCESSION   DATMCV010000543 DATMCV010000000
VERSION     DATMCV010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431334"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VNU03_12515"
     CDS             <1..793
                     /locus_tag="VNU03_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP75335.1"
                     /translation="IEIVHGDTGRVPFGMGTYGSRSASVGGTAIVMSLDKIKEKGKKI
                     AAHLLEANPKDMEYVGGSFVVKGSPQKAIPFGQVALTAYVPHNYPEGVEPGLEETSFY
                     DPSNFCFPFGAHACVVEVDPDTGHVKILRYLAVDDVGNVINPMIVDGMVHGGIAQGVA
                     QALWEEAVYDGDSGQLVSGSMMDYTLPKADMLPFYETDRTETPTPVNPLGVKGAGETG
                     TIASTPAVVNAVVDALSGLGVDHIEAMPMTPERVWKTAQAAKSKK"
     gene            808..1671
                     /locus_tag="VNU03_12520"
     CDS             808..1671
                     /locus_tag="VNU03_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWP75336.1"
                     /translation="MYPAQFEYHTPGTMKEALDLLGRHKDDAKLLAGGHSLLPAMKLR
                     LAQPKHLVDLRKVSGLSGIKEEGGTLVIGAMTTHYQVESSSVVKSKWPVLAAVAAQIG
                     DPMVRNMGTIGGSLAHADPAADYPAAIIALGAEMVAEGPKGKRTIKADDFFKGLLTTA
                     LAEDEILTEVRVPALAAKTGAAYMKFPHPASRFAVVGVAAVLSVDGGKVGKASVGITG
                     AGTKAVRAKGVEAAITGKGTDAASIQAAAEKAAEGVDVQADLQGSVEYKSHLLRVFAK
                     RAMEAAAAAVK"
     gene            1763..2359
                     /locus_tag="VNU03_12525"
     CDS             1763..2359
                     /locus_tag="VNU03_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWP75337.1"
                     /translation="MLAAGLARRMGQAKLLLPVGGQPIVRHAVEGVRAAGLDPVLVVT
                     GPAPAPIEAALAGLDVRIVVNPSPEAGQAGSVRAGIAALPETVDTVLVALGDQPALAP
                     AIIPALLAAHRASGRPIVAPRYQDGPGNPVLFDRAIFAELLALQGDQGARPVIAREPA
                     RVEWVTLDLPMPQDVDTPADYEKIRSALDLPTRPNHAV"
BASE COUNT          426 a          874 c          915 g          329 t
ORIGIN      
        1 catcgagatc gtccacggcg acaccggccg ggtgccgttc ggcatgggca cctacggcag
       61 ccgctcggcc tcggtgggcg gcaccgccat cgtgatgtcg ctcgacaaga tcaaggagaa
      121 gggcaagaag atcgcggccc acctcctcga ggcgaatccg aaggacatgg agtacgtcgg
      181 cggcagcttc gtggtcaagg ggtcgcccca gaaggcgatc ccgttcggcc aggtcgcgct
      241 gaccgcgtac gtgccgcaca actacccgga gggcgtggag cccggtctcg aggagacaag
      301 cttctacgat ccctccaact tctgcttccc gttcggggcc cacgcctgcg tggtggaggt
      361 ggatccggac accggccacg tgaagatcct ccggtacctc gcggtcgacg acgtcggcaa
      421 cgtgatcaac ccgatgatcg tggacggcat ggtgcacggg ggcatcgccc agggcgtcgc
      481 gcaggcgctg tgggaagagg cggtctacga cggagactcc ggccagctcg tgagcggcag
      541 catgatggac tacacgctgc ccaaggccga catgctgccc ttctacgaga ccgatcgcac
      601 cgagacgccc accccggtca atccgctggg cgtgaagggc gcgggagaga ccggcaccat
      661 cgcctcgacg ccggcggtgg tcaacgcggt ggtggacgcc ctgtccgggc tcggcgtgga
      721 tcacatcgaa gccatgccga tgacgccgga gcgggtctgg aagaccgccc aggccgccaa
      781 atcgaagaag taagggagac gccgacaatg tacccagccc agttcgagta ccacacgccg
      841 gggacgatga aggaggcgct ggacctgctc gggcgccaca aggacgacgc caagctgctg
      901 gccggcgggc acagcctgct gcccgccatg aagctgcgcc tggcccagcc caagcacctc
      961 gtggacctcc gaaaggtctc cggactctcg ggcatcaagg aggagggcgg caccctggtc
     1021 atcggggcga tgacgacgca ctaccaggtc gagtcctcgt cggtggtcaa gtcgaagtgg
     1081 ccggtcctgg ccgcggtggc ggcccagatc ggcgacccga tggtgcgcaa catgggcacg
     1141 atcggcggca gcctcgccca cgccgacccg gcggcggact acccggccgc gatcatcgcg
     1201 ctcggcgccg agatggtggc tgagggcccc aagggcaagc gcacgatcaa ggccgacgac
     1261 ttcttcaagg gcctgctcac caccgcgctg gcggaggacg agatcctgac cgaggtacgc
     1321 gtgcccgcgc tcgcggccaa gaccggcgcc gcgtacatga agttcccgca cccggcctcg
     1381 cgcttcgcgg tggtgggagt ggcggcggtg ctgagcgtgg acggcggcaa ggtgggcaag
     1441 gccagcgtgg gcatcaccgg cgccggcacc aaggcggtgc gcgccaaggg cgtcgaggcc
     1501 gcgatcaccg gcaaggggac cgacgccgcg tccatccagg cggcggccga gaaggcggcc
     1561 gagggcgtgg acgtgcaggc cgacctgcag ggatcggtgg agtacaagag ccacctgctg
     1621 cgcgtgttcg ccaagcgcgc gatggaggcg gccgccgcgg ccgtgaagta agcctcctgt
     1681 tgagccgacc gatcgacccc gagccccgcg ggaagcgtcc cgcggggttc ttctttgggg
     1741 atcggccctg atcgcggcgc tggtgctggc cgcgggcctc gcgcgccgga tggggcaggc
     1801 caagctgctg ctcccggtgg gcgggcaacc gatcgtgcgc cacgcggtcg agggagtccg
     1861 ggccgcgggg ctcgaccccg tgcttgtcgt caccgggccc gcgcccgccc cgatcgaggc
     1921 ggcgctggcc gggctcgatg tacggatcgt ggtgaatccg agccctgagg ccggccaggc
     1981 cggctcggtg cgcgccggca tcgccgcgct gcccgagacg gtggacacgg tgctggtcgc
     2041 gctgggcgac cagccagcgc tggccccggc gatcatcccg gcgctgctgg cggcccaccg
     2101 cgcgagcggc cgaccgatcg tcgcgccccg ctaccaggac gggcccggca acccggtgct
     2161 cttcgaccgc gccatcttcg ccgagctcct ggcgctccag ggcgaccagg gcgcgcgccc
     2221 ggtcatcgcc cgggagcccg cccgcgtgga atgggtgacc ctcgacctgc cgatgccgca
     2281 ggacgtcgat acccccgccg actatgagaa gatccggagc gcactcgacc tgccaacccg
     2341 tcccaaccac gcggtgtaga ctgagagggc catgagaccc gagatcgaga agatccagca
     2401 gatgatggaa ggcgccgact acgtcaccga tcccgccatc gccacgtcgg tgcacctggc
     2461 catgaccctc aagaagcctc tcctcatcga ggggcacgcg ggcgtgggca agaccgagat
     2521 cgccaaggtg atgtcgcgga tgct
//
LOCUS       DATMCV010000544         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_27068, whole genome shotgun sequence.
ACCESSION   DATMCV010000544 DATMCV010000000
VERSION     DATMCV010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27068"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /gene="leuS"
                     /locus_tag="VNU03_12530"
     CDS             complement(<1..1859)
                     /gene="leuS"
                     /locus_tag="VNU03_12530"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWP75338.1"
                     /translation="MALDERPERYPFPEIEARWQKAWADSKQFKVTEAPGRPKYYCLE
                     MFPYPSGRIHMGHVRVYAIGDLLARYKWMRGFNVLHPMGWDAFGLPAENAAIDNGVHP
                     AVWTYENIDNMRIQLKRMGISYDWDREVTTCDPAYYKWEQLIFIRMLERGLAYRRRSR
                     VNWCPSCQTVLANEQVEAGRCWRCDSEVVIKEVDGWFFKITDYAQELLDWCDRLPGWP
                     ERVLTMQRNWIGRSEGAEFALPVAGRPDVKIAIYTTRPDTSFGMTYAVLSPEHPLVDT
                     LAVDGEERRAVDTFRAEVARESEIERLATDRPKRGCRLRARAVNPFTGQEIPLFLADY
                     VLMGYGTGAIMAVPGEDQRDWDFAKAHDLPIIATVKRPDDWADEQAYTGDGVKINSGF
                     LDGLGIEEAKRNAIDWLVAQGLGQAKVNFRLRDWGISRQRYWGAPIPVLYCEKDGMVP
                     EREENLPVVLPRDVQISGKGGSPLAEVAGFVNATCPKCGGRARRDTDTMDTFVESSWY
                     FLRYCSPDYDRGMFDPAAAAYWMPVDQYIGGIEHAVLHLLYARFYTKVLRDLGMLKVD
                     EPFHALLSQGMVIKDGAKMSKSKGNVVDPDELIRRFGADTARLFSLFAAPPEK"
     gene            complement(1945..>2144)
                     /locus_tag="VNU03_12535"
     CDS             complement(1945..>2144)
                     /locus_tag="VNU03_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005900956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75339.1"
                     /translation="ALANQCPLLIKQIQDATAGKTDDASKKANALAAEAKALHDSGKH
                     AESVAKADEAAKAINLTLKKK"
BASE COUNT          305 a          729 c          744 g          366 t
ORIGIN      
        1 tccttctcgg ggggcgccgc gaagagggag aagaggcgcg cggtgtcggc cccgaaccgg
       61 cggatgagct cgtcgggatc gaccacgttg cccttcgact tcgacatctt cgccccgtcc
      121 ttgatgacca tgccctggga gagcagggcg tggaagggct cgtcgacctt gagcatgccg
      181 aggtcccgga ggaccttggt gtagaagcgc gcgtagagca ggtgcaggac cgcgtgctcg
      241 atgcccccga tgtactggtc gaccggcatc cagtaggccg ccgcggccgg gtcgaacatg
      301 ccccggtcgt agtcgggcga gcagtagcgc aggaagtacc aggacgactc cacgaaggtg
      361 tccatggtgt cggtgtctcg ccgcgccctg cccccgcact tggggcacgt ggcgttgacg
      421 aaacccgcca cctcggccag cggggagccc cccttcccgc tgatctgcac gtcgcggggc
      481 agcaccaccg ggaggttctc ctcccgctcc ggcaccatgc cgtccttctc gcagtagagc
      541 accgggatcg gcgcgcccca gtagcgctgc cggctgatcc cccaatctcg gagccggaag
      601 ttgaccttgg cctggccgag cccctgcgcg accagccagt cgatcgcgtt ccgcttggcc
      661 tcctcgatgc cgagcccgtc gaggaagccc gagttgatct tcaccccgtc gccggtgtac
      721 gcctgctcgt ccgcccagtc gtcggggcgc ttcacggtgg cgatgatcgg cagatcgtgc
      781 gccttggcga aatcccagtc gcgctggtcc tcgcccggca ccgccatgat cgcgccggtc
      841 ccgtagccca tcagcacgta gtcggcgagg aagagcggga tctcctgccc ggtgaacggg
      901 ttgaccgcgc gcgcgcgcag acgacagccc cgcttcggcc ggtcggtggc gaggcgctcg
      961 atctccgact cgcgcgccac ctcggcgcgg aacgtgtcga ccgcccgccg ctcctccccg
     1021 tcgaccgcga gggtgtcgac gagggggtgc tcgggggaca ggaccgcgta ggtcatgcca
     1081 aacgaggtgt cgggccgcgt ggtgtagatc gcgatcttca cgtcgggccg gcccgccacc
     1141 ggcagcgcga actcggcccc ctcgctgcgg ccgatccagt tgcgctgcat ggtcagcacc
     1201 cgctcgggcc agcccgggag gcggtcgcac cagtcgagca gctcctgggc gtagtcggtg
     1261 atcttgaaga accagccgtc gacctccttg atgacgacct cggagtcgca gcgccagcag
     1321 cgccctgcct ccacctgctc gttggcgagg acggtctggc aggagggaca ccagttcacc
     1381 cgggagcgcc ggcggtaggc gaggccccgc tcgagcatac ggatgaagat cagctgctcc
     1441 cacttgtagt aggcgggatc gcaggtggtc acctcgcggt cccagtcgta ggagatgccc
     1501 atgcgcttga gctggatgcg catgttgtcg atgttctcgt aggtccacac cgcggggtgg
     1561 accccgttgt cgatggccgc gttctcggcg ggcaggccga acgcatccca ccccatcggg
     1621 tgcagcacgt tgaacccgcg catccacttg tagcgggcca ggaggtcgcc gatggcgtag
     1681 acccgcacgt ggcccatgtg gatccggccc gaggggtacg ggaacatctc gaggcagtag
     1741 tacttgggcc ggccgggggc ctcggtcacc ttgaactgct tgctgtccgc ccaggccttc
     1801 tgccaccggg cctcgatctc ggggaagggg tagcgttccg ggcgctcgtc gagggccata
     1861 ttggccctga tttttgcacg cttaggcggg gagggcaaga aacggctccc cgggggccga
     1921 ctcggccccc gggggcggag cgacctactt cttcttcagg gtgaggttga tcgccttggc
     1981 ggcttcgtcc gccttcgcca ccgactcggc gtgcttgccg gagtcgtgca gggccttggc
     2041 ctcggcggcc agagcattcg ccttcttgga tgcgtcgtcg gtcttgccgg ccgtcgcgtc
     2101 ctggatctgc ttgatcagga gcgggcactg gttggccagc gccg
//
LOCUS       DATMCV010000545         3148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_296389, whole genome shotgun sequence.
ACCESSION   DATMCV010000545 DATMCV010000000
VERSION     DATMCV010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296389"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VNU03_12540"
     CDS             <1..1020
                     /locus_tag="VNU03_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWP75340.1"
                     /translation="ERGAADSLPGVGRPGQPAAPHLRRLDFPVPSRAGLPSIKKYAHL
                     ERDGRMELAAYVESSRGCLHRCRHCPIPPVYGGRFFAVPREVVLADVRQQIEAGARHV
                     TFGDPDFLNGPSHALAVARDLHAEWPDVTFDFTAKIEHLLRHRAHLPALREAGCLFVV
                     SAAESLSDTVLAHLDKGHSRADILNALALTREAGITLRPTWVAFTPWTTLDDHCAWLD
                     FLAAESLIDHVDPVQYGLRLLIPPGSLLLETEALRPHLGPLEQATLSYRWTHPDPRMD
                     RLQAETAQMVAQAVEVQADTPGIFDRVRAMAAEAAGAPAPAPLRGEMAKGRKRPPRLS
                     EAWFC"
     gene            1095..2192
                     /locus_tag="VNU03_12545"
     CDS             1095..2192
                     /locus_tag="VNU03_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75341.1"
                     /translation="MARIELTNVCATLQGGERAPGSRGSTFSIRDLSLTVFDGETLVI
                     LGPSGCGKTTLLKIIAGLIAPESGAVHYDGMSMSDVPPGGRRVGMVFQNYALYPHLTS
                     RTNVLSYFLFRKKTPELGALAQEKYRRTSELMGVELDSLLDRRPTTLSGGEKQRVALG
                     RCITRDPAVFLLDEPFSNLDQALRERYRVNLKILLKQFSISTVYVTHDHVEALILADR
                     LAVMDRGRIEQVGTYQEIYEQPRNVFVAGFLNRHVGTPPINLIDARYLAPGRQLGDVQ
                     VGVRPEDVAVSRDERSDGVAGVVAGRLSLPMLNATVLSVRVDQQELFAHIPGDQSVLA
                     GDRVWLTFRKCHLFDKQSGARLGSHAEPAPA"
     gene            complement(2193..2726)
                     /locus_tag="VNU03_12550"
     CDS             complement(2193..2726)
                     /locus_tag="VNU03_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HWP75342.1"
                     /translation="MHPNAMLPLDLEKITAAIASGTRPNQVLWQTEDSIAFVARGRAG
                     RSEFHVDPSDEVMYMIKGDMNLHYRTPEGEEKVAVIREGGIIHCPAGTPHSPRFATDA
                     FVLVIERKRRTGEQDRFFWYCPSCKAQLYEAARHVGDYLQDPVSEVYAEFYGSESHRT
                     CGKCGHVTPRLEAARRA"
     gene            complement(2741..>3148)
                     /locus_tag="VNU03_12555"
     CDS             complement(2741..>3148)
                     /locus_tag="VNU03_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HWP75343.1"
                     /translation="DFIRVKGGRMDRVIMSHIDRTVFDDERLRRLAGTGCVLEWDLFG
                     QESSYYRHSDIDMPNDAVRLRAIRGLIDRGHLAQVLISHDICYRSRLVRWGGHGYGHI
                     FANVVPMMGRRGFSEAEIQAILVDNPRRLLTLV"
BASE COUNT          465 a         1111 c         1089 g          483 t
ORIGIN      
        1 gagcgtggcg ccgcggactc tctgcccggg gtgggccggc cgggccagcc ggccgcgccg
       61 catctccgac ggctcgattt cccggtgccg agccgtgcgg ggctgccgtc gatcaagaag
      121 tacgcgcacc tcgagcgcga cggccgcatg gagctggccg cctacgtgga gtcgagccgc
      181 gggtgcctgc accgctgccg gcactgcccg atcccgccgg tgtacggcgg gcgcttcttc
      241 gcggtgccgc gcgaggtcgt gctggccgac gtgcgtcagc agatcgaggc gggcgcgcgc
      301 cacgtcacct tcggcgaccc ggacttcctc aacgggccga gccacgccct cgcggtggcg
      361 cgcgatctcc acgccgagtg gcccgacgtc accttcgact tcacggcgaa gatcgagcac
      421 ctgctgcgcc accgcgcgca cctgcccgcg cttcgggagg cgggctgcct gtttgtggtc
      481 tcggcggcgg agtcgctgag cgacaccgtg ctcgcgcacc tcgacaaggg tcacagccgc
      541 gccgacatcc tgaacgcgct ggccctcacc cgcgaggccg gcatcacgct gcgccccacc
      601 tgggtggcgt tcacgccgtg gaccacgctc gacgaccact gtgcgtggct cgacttcctg
      661 gctgccgagt cgctgatcga ccacgtggac ccggtgcagt acggcctgcg gctgcttata
      721 ccgccgggct ccctgctgct cgagaccgag gcactgcggc cgcacctggg tccgctcgag
      781 caggccacgc tctcctatcg ctggacgcac cccgacccgc gcatggaccg gctgcaggcg
      841 gagacggcgc agatggtcgc ccaggcggtg gaggtccagg cggacacgcc gggcatcttc
      901 gaccgcgtgc gcgcgatggc ggcggaagcc gccggcgccc cggcgcccgc gccgctgcga
      961 ggcgagatgg ccaagggacg caagcgcccg ccgcggctca gcgaggcgtg gttctgctga
     1021 gcggagccca cccagggcca gttggccctg acgtcgcaga gccggctcta gcccgtgtgc
     1081 cctgaggcgg ccccgtggcg cggatcgagc tgaccaacgt ctgcgcgacg cttcagggcg
     1141 gcgagcgggc gccgggcagt cggggctcga ccttctccat ccgcgacctg agcctgacgg
     1201 tcttcgatgg cgagaccctc gtcatccttg ggcccagcgg atgcggcaag accacgctcc
     1261 tcaagatcat cgccggcctg atcgcgccgg agtccggagc cgtgcactac gacggcatga
     1321 gcatgagcga cgttccgccc ggtggccggc gcgttggcat ggtcttccag aactacgcgc
     1381 tctacccaca cctgacctcg aggaccaacg tcctgtcgta cttcctgttc cggaagaaga
     1441 ccccggagct gggagcgctg gcgcaggaga agtatcggcg gaccagcgag ctgatggggg
     1501 tcgagctcga tagtctcctg gatcgccggc cgaccacgct ctccggcggt gagaagcagc
     1561 gggtcgcgct cgggcgctgc atcacgcgcg atcccgcggt cttcctcctg gacgagccgt
     1621 tctcgaatct cgaccaggcg ctccgggagc gctaccgcgt caatctgaag atcctgctga
     1681 agcagttcag catctcgacc gtgtacgtga cccacgacca cgtcgaggcc ctgatcctcg
     1741 ccgatcgcct ggccgtcatg gatcgaggcc gcatcgagca ggtcggcacc taccaggaga
     1801 tctacgagca gccgcggaac gtgttcgtgg ccgggttcct gaaccggcac gtggggaccc
     1861 cgcccatcaa tctgatcgac gcgcgctatc tcgcccccgg ccggcagctc ggcgacgtgc
     1921 aggtcggcgt ccgtcccgag gacgtggccg tctcccggga tgagaggtcc gacggcgtcg
     1981 cgggcgtggt tgccgggagg ctgagccttc ccatgctcaa cgcgacggtc ctcagcgtcc
     2041 gcgtggatca gcaggagcta ttcgcgcaca ttccaggaga tcagagcgtc ctggccggcg
     2101 accgggtgtg gctcaccttc cggaagtgcc acctgttcga caagcagtcc ggggcaagac
     2161 tgggcagcca cgcggagcca gccccggcct gatcaggcgc ggcgtgccgc ctccagtcgt
     2221 ggggtgacgt gcccgcactt gccgcacgtg cgatgcgact cgctgccgta gaactccgcg
     2281 tagacctcgg acaccggatc ttgcaggtag tcgcccacgt ggcgcgccgc ctcgtagagc
     2341 tgcgccttgc acgacgggca gtaccagaag aagcggtcct gctcgcccgt tcggcgcttg
     2401 cgctcgatca ccagcacgaa cgcgtcggtg gcaaagcgcg gcgagtgcgg cgtgccggcc
     2461 gggcagtgga tgatcccgcc ctcgcggatc accgcgacct tctcctcgcc ctcgggcgtg
     2521 cggtagtgga gattcatgtc gcccttgatc atgtacatga cctcatcgct cgggtcgacg
     2581 tgaaactcgc tccggcccgc gcggccgcgg gccacgaagg cgatggagtc ctccgtctgc
     2641 cacagcacct ggttgggccg ggtgccggag gcgatggccg cggtgatctt ctcgaggtcc
     2701 agcgggagca tggcgttcgg gtgcatggcg aagcctccgt tcagaccagc gtgagtagcc
     2761 gccgcgggtt gtcgaccagg atggcctgga tctcggcctc cgagaagccg cgccggccca
     2821 tcatcggcac cacgttggcg aagatgtggc cgtagccgtg gcccccccag cgcaccaggc
     2881 gggagcggta gcagatgtcg tgggagatca gcacctgggc caggtgcccg cggtcgatca
     2941 gcccgcggat ggcgcgcagc cggaccgcgt cgttcggcat atcgatgtcg gagtgacggt
     3001 agtaggacga ctcctggccg aacagatccc attcgagcac gcagccggta ccggccagcc
     3061 gccgcagccg ctcgtcgtcg aacacggtgc ggtcgatgtg gctcatgatc acgcgatcca
     3121 tccgcccgcc cttcacgcgg atgaagtc
//
LOCUS       DATMCV010000546         2649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_164478, whole genome shotgun sequence.
ACCESSION   DATMCV010000546 DATMCV010000000
VERSION     DATMCV010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164478"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..762)
                     /locus_tag="VNU03_12560"
     CDS             complement(109..762)
                     /locus_tag="VNU03_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWP75344.1"
                     /translation="MSDSALFRSGLRSLLGTYPAFSLVGEASALPVRDLVRAGSPHIL
                     LVDAQIVGALTICAGLRQNGTRPWVILAGADADQAWAVHALKCGARGILTKSATVETL
                     IKAVRLVHQGEIWASHRVLTLAVEELAARSASSGSADPTIKSRLSQREMDIAQLIASG
                     LSNQEVAHRLNITEATVKAHLTHVFQKLMLRGRGQLAALYHRSLAPLSASNGSASAG"
     gene            1304..2392
                     /locus_tag="VNU03_12565"
     CDS             1304..2392
                     /locus_tag="VNU03_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWP75345.1"
                     /translation="MAITLTACGGAHSLSGSDEGALLREGGSPAGAATRAERAPEPAR
                     PPKEAGAGSAAALNLRLMEQAARSSEESDLPVGTGDLLEISVFEVEELSKIRLRVPGR
                     GVISLPLIGQIQATGRTTAELEEEIRSRLQRKFMHEPQVSVFLQEHNSQRISVIGAVR
                     KGGVFNLTRPLRLADALALAEGLTEEADRQVYVIRRAPIGVVTAAAAGGGAPTGPPAG
                     GETTAEVMAPIDLSELADGREDLNVALRAGDVVHVPRAGSVYVGGSVERPGSFLLRGK
                     TTAQQAIFAAGGVKDVADWSDVRLYRKTPAGKVEVTVYDLEAFEQGKPAPELQRNDVV
                     VVGKHAGKAFFYGFLDFFKGALGVAKGI"
     gene            2389..>2649
                     /locus_tag="VNU03_12570"
     CDS             2389..>2649
                     /locus_tag="VNU03_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75346.1"
                     /translation="MSDTFSRALTNGRDLPAPLPPVRLEAEYRRESEAHFWDYWRVLV
                     RHRRTVAMVFAAAVLTALVWSVTARPVFTGTAMLRIDKEEPRV"
BASE COUNT          405 a          879 c          987 g          378 t
ORIGIN      
        1 cagggtcgga gcgaaggcgc gctgaggcat gggtgcaggc ctccttcggg atcgagaggg
       61 atccggaccg ggacggtggc cccgattatg cggctgtcgc gccggctgtc aacccgccga
      121 ggctgagccg ttgctggccg acagcggggc caggctccga tggtacagcg cggcgagctg
      181 cccacggccc cgcagcatga gcttctggaa gacgtgggtg aggtgggcct tcaccgtcgc
      241 ctcggtgatg ttgaggcgat gcgccacctc ctggttgctc agaccgctcg cgatgagctg
      301 ggcgatgtcc atctcgcgct ggctcagacg gctcttgatc gtgggatccg ccgaaccgga
      361 ggaggcggag cgggcggcca gctcctcgac cgcgagggtc agcacccgat ggctcgccca
      421 gatctcgccc tgatggacga gacggaccgc cttgatcagg gtctccaccg tcgcgctctt
      481 ggtcaggatg ccccgcgcgc cgcacttgag cgcgtgcacg gcccacgcct ggtcggcgtc
      541 ggccccggcc aggatcaccc acggccgcgt cccgttctga cggagcccgg cgcagatggt
      601 cagcgccccc acgatctgcg cgtcgaccag caggatgtgc ggcgacccgg ctcgcacgag
      661 gtcacgcacc ggaagcgcgc tcgcctcgcc caccagggaa aacgcggggt acgtgcccag
      721 gaggctccgg agaccggagc ggaagagtgc gctgtcgctc acgagcgcca cgcgaatcac
      781 cgggcacgag agcgcggccg acggcgcggt cctcccggac ggtgacgcgg tggatttcgt
      841 ggccgatcga gcgacgtcgc ggcggttcaa gcgtgcacct ccgtctgcgt ggtgctgacg
      901 attcaccggg gcgagcggtg ttgtcaagga tgaagtgtgt tgcgggaacg tgacgggggc
      961 gaagacggcc gcgcgctcgc gcggtccgac gcgcaccggg aagcaatcac ccgcgccgag
     1021 ctatacgtcg cgcaacaaat gcgggcgacg agtgcctacc gcgggccttc gaagagtgca
     1081 tggacccgat ggcgatttcg cttcccactg tgggaagggc cgacgcggcg atgaatcccc
     1141 attcggcgac ccgcggtggt cgtacacctg aagtcgtagc cgccgcggca cggagtcgca
     1201 tcgggatgac cttggtctaa ttgacggttc gagacccgcg cgtgaccatg cgctcctcgg
     1261 agggccaacg atgagcaacg cgcgctatgt agtgacgagt ctgatggcga tcacgctgac
     1321 ggcctgtggg ggcgcgcact cactcagcgg ttcggacgag ggggcgctgc tccgggaagg
     1381 cggatcgccc gccggcgcgg ccacccgcgc ggagcgcgcg ccggagccgg cgcggccgcc
     1441 caaggaggcc ggcgccggga gcgcggcggc gctcaatctc cggctgatgg agcaggccgc
     1501 gcggtcgagc gaagagagcg atctgccggt ggggaccggc gatctgctcg agatctcggt
     1561 cttcgaggtg gaagagctgt cgaagatccg gctgcgcgtg cccgggcgcg gcgtcatctc
     1621 gcttcccctc atcggccaga tccaggccac cggccgcacc accgccgagc tggaagagga
     1681 gatccgctcc cggctccagc ggaagttcat gcatgagccc caggtatcgg tgttcctgca
     1741 ggagcacaac agccagcgga tctccgtcat cggggcggtc cggaagggcg gcgtgttcaa
     1801 tctcacccgg ccgctccgcc tggccgacgc gctcgccctc gcggagggct tgaccgagga
     1861 ggcggaccga caggtgtacg tgatccggcg cgcgccgatc ggcgtggtca ccgcggcggc
     1921 cgcgggcggg ggcgcgccga ccggcccgcc ggcgggcggc gagacgaccg ccgaggtgat
     1981 ggcgcccatc gatctctccg agctcgccga cgggcgcgag gacctgaacg tcgcgctgcg
     2041 ggccggcgac gtggtgcacg tgccgcgggc cggatccgtg tacgtggggg gctcggtcga
     2101 gcggcccggg tcgttcctgc tgagagggaa gaccaccgcc cagcaggcga tcttcgccgc
     2161 gggaggggtc aaggacgtgg cggactggag cgacgtgcgc ctctatcgaa agacgccggc
     2221 cgggaaggtc gaggtcaccg tctacgatct cgaggcgttc gagcagggca agcccgcgcc
     2281 cgagctgcag cgcaacgacg tggtggtggt cggcaagcac gccggcaagg ccttcttcta
     2341 cggattcctc gacttcttca aaggcgccct cggcgtggcg aagggaatat gagcgataca
     2401 ttcagtcgcg cgctcacgaa cggacgggat ctcccggcgc cgctcccgcc ggtccggctc
     2461 gaggccgagt accgccggga gagcgaggcg cacttctggg actactggag ggtgctcgtc
     2521 cggcaccgcc ggacggtggc gatggtcttc gcggccgccg tcctcaccgc cctcgtttgg
     2581 agcgtgaccg cccggcccgt gttcacgggt accgcgatgc tccgcatcga caaggaggag
     2641 ccccgggtt
//
LOCUS       DATMCV010000547         5279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_496294, whole genome shotgun sequence.
ACCESSION   DATMCV010000547 DATMCV010000000
VERSION     DATMCV010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496294"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VNU03_12575"
     CDS             complement(<1..545)
                     /locus_tag="VNU03_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWP75347.1"
                     /translation="MTVKLKRRRLPSSRRRPRAAGDSLRDTLVDAAVALIARKGPQGF
                     SLREVARRARVSEAAPYWHFADREALLAAVAERGFVELARQMKVIWSRPAAPGERFRA
                     LGVGYVHFAVAHPSYLRVMFGSEVPDKAVHPALEEAATRTFGLLVQAITECQAAKQVR
                     GGDAQTLAVAAWSIVHGLAALL"
     gene            624..1598
                     /locus_tag="VNU03_12580"
     CDS             624..1598
                     /locus_tag="VNU03_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75348.1"
                     /translation="MIRHHVLRGTLLIGALAALVATGCGQIAVWAAPEKTASATRTAA
                     AVEADKVFWATLHGGAYDRIPEALTVLKAAYLANPNDPVTASHIGWMHIWRLGERARH
                     EPVRPDITDDAVLARKYFEEAVRLDPREARYLGFYASLLMAEGTIHKDEKMVRRGFFT
                     MKDAVAAWPEFNLFTAGYTASTQPAESARYREALEQQWRNLDVCVGETVDRARPDYAR
                     YMRLETREGPKRVCWNSWIAPHNFEGFFLNFGDMLVKAGQPDTAVTMYGNARLAREFP
                     AWPYRAVLEARIAGAAANVEAFRKADPAPGAPTLMIRSPFACTACHQE"
     gene            complement(1602..2369)
                     /locus_tag="VNU03_12585"
     CDS             complement(1602..2369)
                     /locus_tag="VNU03_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75349.1"
                     /translation="MTSLAGRVALVTGASRGTGVDIARALASAGARVAVHYRSDRAAA
                     EALAASLRAGGADAAIFGADVARPEDVRRLVAEVEARLGAPAVVVNNAGPFNDTRFGE
                     LAEADWDYVMSANLKAVYLTAQLVAPAMRLEGWGRIVNIGATSGRVRTHGVYGLAKAA
                     LLPLTEALALELAPEITVNAVVPSQIASPRTDGMPDYKAAAIAGTPLGRLVTQAEIGR
                     MVVRLCSPEFDFVTGQAIVMDGGRTIPRFPRLDVSQK"
     gene            complement(2366..2689)
                     /locus_tag="VNU03_12590"
     CDS             complement(2366..2689)
                     /locus_tag="VNU03_12590"
                     /inference="COORDINATES: protein motif:HMM:NF023070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75350.1"
                     /translation="MAIVRYVLAKLKPGVSREDYETFEREVDYVVAGRLKTIVSYRTY
                     RITATDARLAGGPWDYIERIEITDRAAYETELGSMGKEMIDELYAKYLDRSYTTSIWS
                     ELVEP"
     gene            complement(2747..3703)
                     /locus_tag="VNU03_12595"
     CDS             complement(2747..3703)
                     /locus_tag="VNU03_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75351.1"
                     /translation="MNALVVDDLQMHFPLRSIVPWRAPRVVRAVDGVSLRVAAGETLG
                     LVGESGCGKSTLARCVLRLIEPTRGRVLLDGEDLTALPEEALRRRRRDIQMVFQDPTA
                     SLNPRLSVAATVEEPLALHTPLAGPARRARADEVLDEVGLGAGLRDRYPHQLSGGQRQ
                     RVNIARAIATRPRLVVLDEPTSALDVSLRARVILLLAELKRRMGMTYLFISHDLSTVR
                     YLCDRVAVMYLGVLVEEAPAAELFDRPAHPYTRALLSCIPVPDPDVVPARLTLSGEVP
                     SPIDIPTGCRLRGRCPLAQPVCAEPVPVREIAPGHTVACHLV"
     gene            complement(3700..4665)
                     /locus_tag="VNU03_12600"
     CDS             complement(3700..4665)
                     /locus_tag="VNU03_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75352.1"
                     /translation="MTDLLTVRDLTVHFDTDEGVVQAVDGASLRVGPGEVVGLVGESG
                     SGKSVTALAILRLLRPPARVLGGRIELDGRDLLGCSEEEMRGVRGAQISMVFQSPRTS
                     LNPVLPVGHQIERLLVQHGATARGKARARAVEMLREVGIGEPERRARQYAHQLSGGMC
                     QRVMIAMALATNPRLLIADEPTTGLDVSIAAQILDLLGDLGRRTGAAILLITHDLGVV
                     ARRCDRVVVMHAGQTVEWAPVRALFHAPAHPYTRALLRSIPRVVGAGEMEPIPGWVPS
                     LLAPPPGCRFAPRCALVRTECAATVPVRSLDGDHRVACVAVEASW"
     gene            complement(4662..>5279)
                     /locus_tag="VNU03_12605"
     CDS             complement(4662..>5279)
                     /locus_tag="VNU03_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75353.1"
                     /translation="GRVDLLVAVEATAIALSLGAVLGAVSGYRGGWIDQILMRIIDTI
                     QAFPSFILAMGIAAALGNSVTNIVIAIAITHVPIYGRLIRGEMLRIREMEYAEAARTV
                     GNRQRRIIFYHLLPNCFPPVVVQATLAMGFAILTVAALSYIGLGIRPPTSEWGAMTAE
                     GAGYIVSGEWWIFAFPGMAIMATVLSFNLLGDALRDVLDPRMKGV"
BASE COUNT          795 a         1950 c         1854 g          680 t
ORIGIN      
        1 agcagcgcgg ccaggccgtg cacgatcgac caggcggcca ccgcgagggt ctgggcgtcg
       61 ccgccccgca cctgtttcgc cgcctggcac tcggtgatcg cctggaccag aagcccgaag
      121 gtccgcgtcg ccgcttcttc cagggccggg tgtaccgcct tgtccgggac ctcggaaccg
      181 aacatcactc gcagatacga aggatgagcg accgcgaagt ggacgtagcc gacgcccagc
      241 gcgcggaagc gctcgcccgg tgcggcgggt cgcgaccaga tcaccttcat ctgcctcgcc
      301 agctccacga agccccgctc cgcgaccgcg gccagcagcg cctcccgatc ggcgaagtgc
      361 cagtagggcg cggcctccga gacgcgcgcc cgccgcgcca cctcgcgcag gctgaagccc
      421 tggggtccct tgcgcgcgat gagcgccacc gccgcgtcca ccagggtgtc gcgcaggctg
      481 tcgccggccg cgcgggggcg gcgacgggac gacggtagac ggcggcgctt gagcttgaca
      541 gtcataactt aacagcgtta atgtccgcgt tgacgacggg ctcgtcgggg cacgtgctcc
      601 gcaaccccgc gcgggagggt accatgatcc gccatcacgt gctccgggga acattgctga
      661 tcggcgccct cgccgcgctc gtcgcgacgg gctgcggaca gatcgcggtc tgggccgcgc
      721 ccgagaagac cgcgagtgcc acgcgcaccg ccgccgccgt cgaggccgac aaggtcttct
      781 gggccacgct gcacggcgga gcctacgacc ggatcccgga ggcgctcacc gtgctgaagg
      841 ccgcctacct ggccaacccc aacgacccgg tgaccgcctc tcacatcggc tggatgcaca
      901 tctggcgtct cggcgagcgt gcgaggcacg agccggtccg gcccgacatc accgacgacg
      961 cggtgctcgc gcgaaagtac ttcgaggagg cggtgcgcct cgatccgcgg gaggcgcgct
     1021 acctgggctt ctacgcgagc ctgctgatgg ccgagggcac catccacaag gacgagaaga
     1081 tggtgcggcg cgggttcttc accatgaagg acgcggtggc cgcgtggccg gagttcaacc
     1141 tgttcacggc cggctacacc gcctcgaccc agcccgccga gtccgcgcgc taccgcgagg
     1201 ccctcgagca gcaatggcgc aacctcgacg tgtgcgtcgg cgagaccgtc gaccgcgcgc
     1261 gccccgacta cgcgcgctac atgcgcctcg agacccgcga gggccccaag cgagtgtgct
     1321 ggaactcgtg gatcgccccg cacaacttcg agggcttctt cctcaacttc ggcgacatgc
     1381 tggtcaaggc gggccagccc gataccgcgg tgacgatgta cgggaacgcc cgcctcgcgc
     1441 gggaattccc ggcgtggccc tatcgtgcgg tgctcgaggc ccgcatcgcc ggcgcggccg
     1501 ccaacgtgga ggccttccgg aaggcggatc cggccccggg cgcgccgacc ctcatgatcc
     1561 gctccccgtt cgcttgcacc gcctgccacc aggagtagcg gtcacttctg cgagacgtcg
     1621 agccgcggaa agcgcgggat tgtccggccg ccgtccatca cgatggcctg gccggtgacg
     1681 aagtcgaact cgggggagca gagccggacc accatccggc cgatctccgc ctgggtcacg
     1741 aggcggccca gcggcgtgcc ggcgatcgcg gccgccttgt agtccggcat gccgtcggtt
     1801 cgcgggctcg cgatctggct cgggaccacc gcgttcacgg tgatctcggg ggccagctcc
     1861 agcgcgagcg cctcggtcag cgggagcagc gccgccttgg ccaggccgta gacgccgtgg
     1921 gtgcgcacgc ggcccgacgt cgcgccgatg ttcacgatgc gcccccagcc ctcgagccgc
     1981 atggccggcg ccaccagctg ggccgtcagg tagaccgcct tgaggttggc gctcatcacg
     2041 tagtcccagt ccgcctcggc cagctcaccg aaccgcgtgt cgttgaacgg gcccgcgttg
     2101 ttcaccacca ccgcgggtgc gccgaggcgc gcctccacct cggccaccag gcggcgcacg
     2161 tcctcaggcc gcgccacgtc ggctccgaag atcgcggcgt cggcgccgcc ggcgcgaagg
     2221 ctcgcggcca gcgcctccgc ggcggcgcga tcggagcgat agtgcacggc cacccgcgcg
     2281 cccgccgagg ccagggcgcg cgcgatgtcc actccggtgc cacgcgaggc gcccgtcacc
     2341 agggcgacgc gcccggccag gctcgtcacg gctcgaccag ctccgaccag atcgacgtgg
     2401 tgtagctccg atcgaggtac ttggcgtaga gctcgtcgat catctccttc cccatcgagc
     2461 ccagctccgt ctcgtaggcg gcgcggtcgg tgatctcgat gcgctcgatg tagtcccacg
     2521 gaccgcccgc gaggcgggca tcggtggcgg tgatccggta cgtgcggtag ctcacgatcg
     2581 tcttgagccg tcccgcgacc acgtagtcca cctcccgctc gaacgtctcg tagtcctccc
     2641 ggctgactcc cggtttcagt ttcgcgagca cgtatcgcac gatcgccatc ggagatctcc
     2701 tcgctcgccg cgaggcgagc ggcagcggtg aacggcaggc ccgggctcag acgaggtggc
     2761 aggccaccgt gtggccgggc gcgatctccc gcaccggcac cggctcggcg cacaccggct
     2821 gggcgagcgg gcagcggccg cgaagccggc acccggtcgg aatgtcgatc gggctcggca
     2881 cctccccact cagggtgagg cgcgcgggca cgacgtcggg atccgggacc ggaatgcacg
     2941 acaggagcgc gcgggtatag ggatgcgccg ggcgatcgaa gagctcggcg gcgggggcct
     3001 cctccaccag cacgccgagg tacatcaccg ccacgcggtc gcacaggtag cgcacggtgg
     3061 aaaggtcgtg cgagatgaag aggtacgtca tgcccatgcg gcgcttgagc tcggcgagca
     3121 gcaggatcac ccgggcacgc agcgagacgt cgagcgccga ggtcggctcg tcgagcacga
     3181 ccaggcgcgg gcgggtggcg atggcccgcg cgatgttcac gcgctgacgc tgccccccgg
     3241 agagctggtg ggggtagcgg tcccgcaggc ccgcgccgag gccgacctcg tccaggacct
     3301 cgtccgcccg cgcgcgtcgc gccgggccgg ccaggggcgt gtggagcgcg agcggctcct
     3361 ccacggtggc cgccacgctc agccgcgggt tcagcgaggc ggtcgggtcc tggaacacca
     3421 tctggatgtc ccgccggcgc cggcgcagcg cctcctccgg gagcgcggtg agatcctccc
     3481 cgtccaggag gacacggcct cgggtcggct cgatgagacg gagcacacac cgcgcgagcg
     3541 tggatttccc gcagccggac tcccccacca ggcccagcgt ctcgccggcg gccacgcgga
     3601 gcgagacccc gtcgaccgcg cgcaccacgc ggggcgcccg ccacgggacg atcgagcgga
     3661 ggggaaagtg catctggagg tcgtcgacca ccagcgcgtt caccacgagg cctccaccgc
     3721 cacgcacgcc acccggtggt cgccgtcgag cgaccgcacc ggaaccgtgg ccgcgcactc
     3781 ggtccggacc agcgcgcagc gcggggcgaa tcggcagccg ggcggcggcg ccagcaggga
     3841 cggcacccag ccggggatcg gctccatctc ccccgccccg acgacgcgcg gaatggaccg
     3901 caagagagcc cgcgtgtagg gatgggcggg agcgtggaag agggcccgca ccggcgccca
     3961 ttcgacggtc tggcccgcgt gcatcaccac gactcggtcg cagcggcgcg cgaccacccc
     4021 cagatcgtgt gtgatcagca agatcgccgc gccggtccgg cggccgaggt cgcccagcag
     4081 gtccaggatc tgggccgcga tcgagacgtc gagcccggtg gtcggctcgt ccgcgatcag
     4141 cagccgcggg ttcgtggcga gggccatcgc gatcatcacc cgctggcaca tgccgccgga
     4201 gagctgatgg gcgtattgcc gggccctccg ctccggctcg ccgatgccga cctcgcgaag
     4261 catctcgacc gcgcgcgcgc gggccttccc gcgggcggtc gcgccgtgct gcacgagcag
     4321 gcgctcgatc tggtggccga ccggcagcac cgggttgagc gacgtccgcg ggctctggaa
     4381 caccatcgag atctgcgccc cgcgcacccc gcgcatctcc tcctcggagc accccagtag
     4441 gtcccgcccg tcgagctcga tccggccacc cagtacccgc gcgggcgggc ggagcaggcg
     4501 caggatggcg agcgcggtca ccgacttgcc cgagcccgac tcgccgacca ggcccaccac
     4561 ctcgcccggg ccgacccgga ggctcgcccc gtccaccgcc tgcaccacgc cctcgtcggt
     4621 gtcgaagtgc accgtgaggt cgcggaccgt gagcaggtcg gtcacacgcc cttcatccgc
     4681 gggtccagca cgtcccgcag cgcgtcgccc agcaggttga acgagagcac ggtcgccatg
     4741 atcgccatgc cgggaaaggc gaagatccac cactcgccgg agacgatgta accggccccc
     4801 tccgcggtca tcgcgcccca ctcgctcgtg ggcggccgga tgccgagccc gatgtaggag
     4861 agcgcggcca ccgtgaggat cgcgaagccc atcgcgaggg tggcctggac gaccaccggc
     4921 gggaagcagt tcggcagcag gtggtagaag atgatccgcc gctggcggtt gcccaccgtg
     4981 cgggccgcct ccgcgtactc catctcgcgg atgcgcagca tctccccgcg gatgaggcgg
     5041 ccgtagatgg gcacgtgggt gatcgcgatc gcgatcacga tgttggtgac gctgttgccg
     5101 agggccgccg cgatccccat cgcgaggatg aacgacggaa acgcctggat ggtgtcgatg
     5161 atccgcatca ggatctgatc gatccagccg cctcgatagc cggacaccgc gccgagcacc
     5221 gccccgaggg agagcgcgat cgcggtcgcc tcgacggcca ccagcaggtc cactcgccc
//
LOCUS       DATMCV010000548         2432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_561482, whole genome shotgun sequence.
ACCESSION   DATMCV010000548 DATMCV010000000
VERSION     DATMCV010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561482"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VNU03_12610"
     CDS             complement(<1..762)
                     /locus_tag="VNU03_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP75354.1"
                     /translation="MLARAPAGSSLWACTAIGTAAVVAVPIVAVLSSLVVPSGDVWRH
                     LWRTQLLELIANTLLLLLGVGTGTLLVGGGLAWLVVFHRFPGRSVLEWALILPLAVPA
                     YVMGFTFLGLFEYAGPIQGALRGALGAGARLPELRSYGGVVAMMTLVFYPYVYLLARV
                     AFREQGTAAVEIARTLGRSRPAAFLAVTVPMGRPALAAGVALAMMEALADFGTVATFG
                     YRTLTEAIYRVWYGMFDRIAATQLASVLLLFALALL"
     gene            complement(770..1792)
                     /locus_tag="VNU03_12615"
     CDS             complement(770..1792)
                     /locus_tag="VNU03_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP75355.1"
                     /translation="MARARTRTIGTMTLAAALGLATWMVAASGEAAEVIVYSARAHYG
                     QEPAMEAFTRKTGIQVKSFGGEAGPLFERLKAEGDRTPADVLISVDAGHLWNAARAGL
                     LSKVDSPELQANIPAHLRDPESRWVGLTVRARTIMYNTARVKPEELSTYEALGDPKWK
                     GRLCLRTSTHIYNQSLIATTIKRYGEPKTEAIVRGWVANNPTLINGDTKILEAVAAGQ
                     CDVALTNTYYLGRILAKDGAFPVAAFWANQQTTGTHVNISGAGVTTHAKNRAEAIKLI
                     EFLTGPEAQQMFADSNFEFPANPQAAVNPVIAKWGKFKQDDTNVAAAGEFQAAATRLA
                     DRAGYK"
     gene            1918..>2432
                     /locus_tag="VNU03_12620"
     CDS             1918..>2432
                     /locus_tag="VNU03_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HWP75356.1"
                     /translation="MDSRLWAFVVVATLLAVTPGADTLLVVRNVLARGRVAALLTVAG
                     IAGGCVVHALLSAVGVSLILVRSAEAFAVLRFCGAAYLIVLGAQSVRRWWRVDDGALA
                     APDRGAALRPSRRLRSFVEGLLTNVLNPKVGIFYLAFLPQFIVPGDPVLERSLLLAAL
                     HIGIGVVWLSVL"
BASE COUNT          337 a          855 c          867 g          373 t
ORIGIN      
        1 gagcagcgcc agggcgaaga gcagcagcac gctcgcgagc tgggtggccg cgatccgatc
       61 gaacatgccg taccagacgc gatagatggc ctcggtcagc gtgcgatagc cgaacgtggc
      121 gacggtcccg aagtcggcga gcgcttccat catcgcgagc gccacccccg ccgcgagggc
      181 gggccgcccc atcggcaccg tgaccgcgag gaacgcggcc gggcgcgagc ggccgagggt
      241 gcgagcgatc tccaccgcgg cggtgccctg ctcgcggaag gccacccggg ccaggaggta
      301 gacgtacggg tagaagacca gggtcatcat ggccaccacg ccgccgtagg agcgcagctc
      361 gggcagccgg gcgcccgccc cgagcgcgcc gcgcagcgcg ccctggatcg gccccgcgta
      421 ctcgaagagt ccgaggaagg tgaagcccat gacgtaggcc ggcaccgcga gcggcaggat
      481 cagcgcccac tccagcaccg agcgcccggg aaaccggtgg aacaccacga gccaggcgag
      541 gccgccgccg acgagcagcg tgcccgtccc cacgcccagg aggagcagga gcgtgttggc
      601 gatcagctcg aggagctggg tgcgccagag atggcgccag acgtcccccg acgggacgac
      661 cagcgacgag agcaccgcca cgatcgggac cgcgaccacc gccgcggtcc cgatcgcggt
      721 acaggcccag agcgaggacc cggccggcgc gcgggcgagc acccgcgggc tacttgtagc
      781 cggctcgatc cgccagccgc gtggccgcgg cctggaactc gccggccgcc gccacgttgg
      841 tgtcgtcctg cttgaacttg ccccacttgg cgatgaccgg gttcaccgcg gcctgggggt
      901 tggccgggaa ctcgaagttc gagtccgcga acatctgctg cgcctcgggg ccggtcagga
      961 actcgatgag cttgatcgcc tcggcccggt tcttggcgtg cgtggtgacg ccggcgcccg
     1021 agatgttcac gtgggtcccg gtcgtctgct ggttggccca gaaagcggcc accgggaacg
     1081 ctccgtcctt ggccaggatg cgcccgaggt agtaggtatt ggtcagcgcc acgtcgcact
     1141 ggccggccgc gaccgcttcc aggatcttgg tgtccccgtt gatcagggtc ggattgttgg
     1201 cgacccagcc ccgcacgatg gcctcggtct tcggctcccc gtagcgcttg atcgtggtgg
     1261 cgatcagcga ctggttatag atgtgggtcg acgtgcgcag gcacagccgc cccttccact
     1321 tcggatcccc gagggcctcg taggtggaga gctcctccgg cttcacccgg gcggtgttgt
     1381 acatgatggt gcgcgcgcgc accgtgagcc cgacccagcg actctcggga tcgcggagat
     1441 gcgcggggat gttggcctgg agctcgggcg agtccacctt ggacaggagc ccggcgcgcg
     1501 ccgcgttcca gagatggccc gcgtccacgc tgatcaggac gtcggccggg gtccggtcgc
     1561 cctccgcctt cagccgctcg aagagcggcc cggcctcccc gccgaagctc ttcacctgga
     1621 tgccggtctt ccgggtgaag gcctccatcg ccggctcttg cccgtagtgc gcacgcgcgg
     1681 agtacacgat cacctccgcg gcctcgccgc tcgccgccac catccaggtg gccaggccca
     1741 gcgccgccgc cagggtcatc gtcccgatcg tcctcgtccg cgctctcgcc atggtcgtct
     1801 ctcctgggaa acggggtgac ggctgaaatg gagagtgagg ctagcctaat atccggaatg
     1861 tgtcaaacct aattcgaccg gcggggcgag gcggaccgtc gggctacaat gcgggccatg
     1921 gactcgcgcc tctgggcctt cgtcgtcgtg gccaccctgc tggccgtcac gccgggcgcc
     1981 gacaccctgc tggtcgtgcg caacgtgctc gcgcgtgggc gggtcgcggc gctgctcacc
     2041 gtcgccggta tcgcgggtgg ctgcgtcgtc cacgcgctgc tctccgcggt cggcgtctcg
     2101 ctgatcctgg tgcgctcggc cgaggccttc gcggtgctcc ggttctgcgg cgcggcctac
     2161 ctgatcgtgc tcggcgcgca gtcggttcga cgctggtggc gggtggacga cggcgccctc
     2221 gccgcacccg accgcggggc ggcgcttcgc ccgtcgcggc ggctgcgctc gttcgtggaa
     2281 gggctgctca ccaacgtgct caacccgaag gtgggaatct tctacctcgc gttcctgccc
     2341 cagttcatcg tgccggggga tccggtgctc gagcgatcgc tgctgctggc cgcgctgcac
     2401 atcgggatcg gggtggtctg gctctccgtg ct
//
LOCUS       DATMCV010000549         3376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_27262, whole genome shotgun sequence.
ACCESSION   DATMCV010000549 DATMCV010000000
VERSION     DATMCV010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27262"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VNU03_12625"
     CDS             <1..761
                     /locus_tag="VNU03_12625"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWP75357.1"
                     /translation="QSEITDLLQLVREWYATFNLQPSYRLATRPPDKLGTEQQWDQAE
                     DALHQALRANNLVSDLDKGGGAFYGPKIDIDVEDALGRRWQLATIQVDLTMLPERMQC
                     EYIDTDGQAKRPVVIHRAIFGSYERFVAILTEHFAGAFPTWLAPIQARVLPISEKHAE
                     YGRKVFAALRAARIRVELDDRNEKLGYRIRDAQVRKVPYMLVVGEREAQSGAASLRRR
                     TGEDVGAVPVERIVAELTAEISSRSATLTVGRAG"
     gene            807..1307
                     /gene="infC"
                     /locus_tag="VNU03_12630"
     CDS             807..1307
                     /gene="infC"
                     /locus_tag="VNU03_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWP75358.1"
                     /translation="MRINEGIRVREVRVVSADGEQLGILPIQQALDLARQRDMDLVEV
                     AAEAQPPVCRIMDFGKFKYMQARRQKDARKKQTIIQVKEVKLGPKTDTHDFDFKAKHV
                     RRFLEEGNKAKVTVRFKGREMAHTELGWKMLNKMVEGMQDIAVIENHPRMEGRMLSMI
                     LSPKPH"
     gene            1384..1581
                     /gene="rpmI"
                     /locus_tag="VNU03_12635"
     CDS             1384..1581
                     /gene="rpmI"
                     /locus_tag="VNU03_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWP75359.1"
                     /translation="MPKMKTKRSAAKRLKATASGKLKRSSGWKRHKLEGKSPKRKRHL
                     RKPQMISEADQPRLKKLVPYL"
     gene            1598..1954
                     /gene="rplT"
                     /locus_tag="VNU03_12640"
     CDS             1598..1954
                     /gene="rplT"
                     /locus_tag="VNU03_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWP75360.1"
                     /translation="MPRAKGGAKTRQRRKKILKQAKGYFGGRRKLYRPAAETVLRAGA
                     FAYRGRKQKKRRARSLWIIRVNAACRQVGLSYSTFIAGLKKAGIALDRKILAELAVSD
                     PTAFAKLAETARAQRA"
     gene            1957..3000
                     /gene="pheS"
                     /locus_tag="VNU03_12645"
     CDS             1957..3000
                     /gene="pheS"
                     /locus_tag="VNU03_12645"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWP75361.1"
                     /translation="MAGDPRVQEVLERALAEVAGARSTSDLEQVRVRMLGRAGQLTTL
                     LRSLGAIPAAERPRVGEETNRAKASVEAAIAARLEGLKAEEHRRTLAADRPDLTLPGR
                     RPLPGPVHPLTRVTEEIIEVFEGLGFSVAEGPEVESDYYNFAALNFPADHPARDMQDT
                     FHLSADTLLRTHTSPVQIRTMKAQRPPVRIICLGKVYRRDILDATNSPMFHQVEGLAV
                     DTHITMADLKATLQLFAREMFGPKSTVRFRPSFFPFTEPSAEVDVRCFKCGGDGCRFC
                     KESGWLEILGSGMVHPNVLRNVGYDPEEVTGWAFGMGIDRVALLKYEIDDIRLFFDND
                     LRFLQQFGGLPGR"
     gene            2997..>3376
                     /locus_tag="VNU03_12650"
     CDS             2997..>3376
                     /locus_tag="VNU03_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWP75362.1"
                     /translation="MKISYRWLKEFVETDLDARAVADRLTNAGIGVEQITPVVEGLAG
                     VVVGEIEAIERELGTSAAGHQNRLVRVALPERRFEVVCGAPNAAPGLRAAFAPPGARL
                     PGLGEVKAARIRGVVSEGILCSEK"
BASE COUNT          586 a         1147 c         1182 g          461 t
ORIGIN      
        1 tccagtccga gatcacggat ctcctccagc tggtgcgcga gtggtacgcc accttcaacc
       61 tgcagccctc gtaccggctc gccacgcggc cgcccgacaa gctcgggacc gagcagcagt
      121 gggaccaggc ggaggacgcg ctccaccagg ctctgcgcgc gaacaacctc gtctccgatc
      181 tggacaaggg cggcggcgcc ttctacgggc ccaagatcga catcgacgtg gaggacgcgc
      241 tggggcggcg gtggcagctc gccaccatcc aggtggacct cacgatgctg cccgagcgca
      301 tgcagtgcga gtacatcgac acggacgggc aggccaagcg gccggtggtg atccaccggg
      361 cgatcttcgg ctcctacgag cggttcgtgg cgatcctcac tgaacacttt gccggcgctt
      421 tcccgacatg gctggcgccc attcaggcgc gggttctgcc gatcagcgag aagcacgccg
      481 agtacggccg caaggtgttc gcggcgctgc gggccgcgcg catccgcgtc gagctcgacg
      541 accgcaacga gaagctgggc tatcgcatcc gcgacgccca ggttcgcaag gtgccctaca
      601 tgctggtcgt gggggagcgg gaggcccaga gcggcgccgc gagcctgcgc cgacggacgg
      661 gcgaggacgt gggcgcggtg ccggtggagc gcatcgtggc cgagctcacc gccgagatct
      721 ctagccgttc cgccaccctc accgtgggcc gcgccggctg agccggcccc agaaccgaag
      781 gagcgcgcgc catccagtca aaagacatcc ggatcaatga aggcattcgg gtgagggaag
      841 tgcgagtggt gagcgccgac ggcgagcagc tcgggatcct gccgatccag caggccctgg
      901 atctcgcgcg ccagcgggac atggacctgg tcgaggtggc ggcggaggcc cagcctccgg
      961 tctgccggat catggacttc ggcaagttca agtacatgca ggcgcggcgg cagaaggacg
     1021 cgcgcaagaa gcagacgatc atccaggtga aagaggtcaa gctggggccc aagaccgaca
     1081 cccacgactt cgacttcaag gccaagcacg tgcggcgctt cctggaagag ggcaacaagg
     1141 ccaaggtcac ggtacgcttc aaggggcgcg agatggcgca caccgagctc ggctggaaga
     1201 tgctcaacaa gatggtggag ggcatgcagg acatcgcggt catcgagaac cacccccgga
     1261 tggagggccg catgctcagc atgatccttt cgccgaagcc gcactagcgg acaaccttga
     1321 ccacccctca ccctgctctc tcccctgagg ggagagggga atgaggaagg aacgacacac
     1381 catatgccga agatgaagac caagcggtcg gcggccaagc ggttgaaggc gaccgcctcc
     1441 ggcaagctca agcggagcag cggatggaag cggcacaagc tcgagggcaa gagccccaag
     1501 cggaagcgcc acctccggaa gccccagatg atctccgagg cggaccagcc ccgcctgaag
     1561 aaactggttc cgtacctcta acaacaggga gtcagtcatg ccacgggcaa aaggtggggc
     1621 caagacccgc cagcgcagga agaagattct caagcaggcg aaggggtact tcggcgggcg
     1681 gcgcaagctc taccgcccgg ccgcggagac ggtgctccgc gcgggcgcct tcgcgtatcg
     1741 ggggcggaag cagaagaagc gccgggcgcg ctcgctgtgg atcatccggg tgaacgcggc
     1801 ctgccgtcag gtggggctct cgtactccac cttcatcgcc gggctcaaga aggcgggcat
     1861 cgcgctggat cgcaagatcc tcgccgagct cgcggtgtcc gatccgacgg ccttcgccaa
     1921 gctggccgag acggctcgcg cccagcgcgc ctgagcgtgg cgggggaccc gcgggtccag
     1981 gaggtcctcg agcgggccct ggcggaggtc gccggggccc gctccacgtc cgacctcgag
     2041 caggtgcgcg tccgcatgct cgggcgcgcc gggcagctca ccactctgct ccgctccctc
     2101 ggggccatcc cggccgcgga gcggccccgg gtgggcgagg agaccaaccg ggccaaggcc
     2161 agcgtcgagg ccgcgatcgc ggcccgcctc gagggcctga aggcggagga gcaccgccgc
     2221 accctcgcgg cggatcgccc cgacctcacc ctgcccgggc gccgtccgct gcccggcccg
     2281 gtccatccgc tcacccgggt gaccgaggag atcatcgagg tgttcgaggg gctcggcttc
     2341 tcggtcgcgg agggccccga ggtcgagtcg gactactaca acttcgcggc cctgaacttc
     2401 ccggccgacc atcccgcgcg cgacatgcaa gacacgtttc acctctcggc ggacacgctg
     2461 ctccgcaccc acacctcgcc ggtgcagatc cgcaccatga aggcgcagcg accgccggtg
     2521 cggatcatct gcctcggcaa ggtctaccgg cgcgacatcc tcgacgcgac caactcgccg
     2581 atgttccacc aggtggaggg gctcgcggtc gacacgcaca tcacgatggc cgacctgaag
     2641 gccacgctcc agctcttcgc gcgcgagatg ttcgggccga agtccaccgt gcgcttccgc
     2701 ccgtcgttct tcccgttcac cgagccgtcc gccgaggtcg acgtgcgctg cttcaagtgc
     2761 ggcggcgacg ggtgccggtt ctgcaaggag tcgggctggc tcgagatcct gggctcgggc
     2821 atggtccacc cgaacgtgct gcgcaacgtg ggctacgacc cggaggaggt caccggctgg
     2881 gccttcggga tggggatcga tcgcgtcgcc ctgctcaagt acgagatcga cgacatccgg
     2941 ctcttcttcg acaacgatct ccgcttccta cagcagttcg gcggcctgcc cgggcgatga
     3001 agatctccta ccgctggctc aaggagttcg tcgagaccga tctcgacgcg cgcgcggtcg
     3061 ccgaccggct caccaacgcg gggatcgggg tggagcagat caccccggtg gtcgagggcc
     3121 tcgcgggcgt ggtggtgggc gagatcgagg ccatcgagcg cgagctcggg acgagcgcgg
     3181 cgggccacca gaatcgcctc gtgcgcgtcg cgctgccgga gcggcgcttc gaggtcgtct
     3241 gcggtgcccc caacgcggcc ccggggctgc gcgccgcctt cgcgccgccc ggcgcgcggc
     3301 tgccggggct cggcgaggtg aaggcggcca ggattcgcgg cgtggtctcg gaaggcatcc
     3361 tgtgttccga gaagga
//
LOCUS       DATMCV010000550         2853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_431747, whole genome shotgun sequence.
ACCESSION   DATMCV010000550 DATMCV010000000
VERSION     DATMCV010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431747"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1503)
                     /locus_tag="VNU03_12655"
     CDS             complement(157..1503)
                     /locus_tag="VNU03_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP75363.1"
                     /translation="MTTPIRVLVADDEKNLRELLVRELGRKGHEAIGVADGRAALEHL
                     KEDVPDVLLLDMKMPNVEGIEVLRELQDLPEAPQVIVMTGFQEVSTAVEAMKLGAYDY
                     LTKPARIEELEILIRKAAEKGRLMRQNVVMRSQLDEPGYQGIITGSSRMEEVLRLIDR
                     VAPTDSSVLVLGESGTGKELVARAIHDRSPRAQQAFVPIHCGALAREVLESELFGHEK
                     GAFTGAIATKPGLIELADGGTLFLDEIGEMEPESQVKLLRVLESHAFFRVGGTRRRTV
                     DMRLVAATNRDLAEAIKTGGFRQDLFYRINTITITLPPLRERREDVRRLTEHFLEQNA
                     TYGRKRLSDSAMESLERYDWPGNVRELQHAVQRAVILSQGDQIQPGDLPEEIRGGRAT
                     SSTPGGSLEDMERQHIISTLRSVGGHRAKAATVLRIDPKTLYRKIQAYGIVPELYR"
     gene            complement(1500..>2853)
                     /locus_tag="VNU03_12660"
     CDS             complement(1500..>2853)
                     /locus_tag="VNU03_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP75364.1"
                     /translation="LYRDPRVRYPQSGLRSMLAAPLIVGDQAIGALTVLRSDVHHFSA
                     EEESLVSAFADQAAMALEHARLFSSVRTYSERLEAMVEARTHELDEQKRFVEVVLETL
                     PLGLFVLDAELRVISANREGTALVPVGPTARRPFAELMPAAKAEAVRTFLEGVVAARE
                     VQHAEQELPAGGETLTVRLTATPLRAPQATHVLVLVEDITLQKRLERQMLLTERLTTA
                     GRLAAGVAHELNNPLATIAGCAEALRERAQDPHLRDLDAFKDFPGYLTLIEEEAYRCK
                     EITGSLLHFVRDPGSRRAPTDLNVLVDKVLELIGHQSRYAQSRLERRPDAELPDVVAN
                     EGQLRQVFLGLASNALDAMNGKGTLTVTTRRRNAREVEIRFEDQGPGIPEHVLPRVFD
                     PFFTTKPPGQGTGLGLAIAQGIVADHEGRIEVDTRVGAGTTFRVILPLQPAEAGKGKP
                     "
BASE COUNT          382 a         1028 c          981 g          462 t
ORIGIN      
        1 cgtggaacac actctactac ggggggatcg cgggcatgat cgccgccccg atcttcttct
       61 acccttactc gaagctctgc tggctcgcct tcgatctcgg gttccgaccg ccgcgcccgc
      121 aggacttcgc cccgcgcacc acccggtaat ccgccctcac cggtagagct cggggacgat
      181 gccgtaggcc tggatcttgc gatagagggt cttggggtcg atgcggagca cggtggccgc
      241 cttggcccga tgaccgccca cgctgcgcag ggtcgagatg atgtgctgcc gctccatgtc
      301 ctcgagactt ccccccggtg tcgagctcgt ggcccgcccg ccgcgaatct cctccggcag
      361 gtcgccgggc tggatctgat cgccctgcga gaggatcacc gcgcgctgca ccgcgtgctg
      421 cagctcgcgc acgttgccgg gccagtcgta gcgctcgagg ctctccatcg cggaatcgga
      481 gaggcgcttg cggccgtagg tcgcgttctg ctccaggaag tgctcggtca gacggcgcac
      541 gtcctcgcgg cgctcgcgca ggggcggcag ggtgatcgtg atggtgttga tgcggtagaa
      601 caggtcctgg cggaacccgc cggtcttgat cgcctccgcg aggtcccggt tcgtcgcggc
      661 gaccagccgc atgtccaccg tgcgccgccg ggtcccgccc acccggaaga acgcgtgcga
      721 ctcgagcacc cggagcagct tcacctggct ctccggctcc atctcgccga tctcgtcgag
      781 aaacagggtg ccgccgtcgg ccagctcgat gagcccgggc ttggtggcga tcgcgccggt
      841 gaacgccccc ttctcgtggc cgaacagctc gctctccagc acctcccggg cgagcgcgcc
      901 gcagtggatg ggcacgaacg cctgctgcgc gcgcggggag cggtcgtgga tggcccgcgc
      961 caccaactcc ttgccggtac cgctctcgcc cagcaccagc accgacgagt cggtcggggc
     1021 gacgcgatcg atgaggcgga gcacctcctc catccgggag ctgccggtga tgatcccctg
     1081 gtagcccggc tcgtcgagct gggagcgcat caccacgttc tggcgcatga ggcggccctt
     1141 ctcggccgcc ttgcggatca ggatctcgag ctcctcgatg cgggccggct tggtcaggta
     1201 gtcgtacgct ccgagcttca tggcctcgac cgcggtggac acctcctgga agccggtcat
     1261 cacgatgacc tggggcgcct ccgggagatc ctgcagctcg cgcagcacct cgatgccttc
     1321 cacgttcggc atcttcatgt cgagcaggag gacgtcgggg acgtcctcct tgaggtgctc
     1381 gagcgcggcc cggccgtcgg ccaccccgat ggcctcgtgg cccttccggc cgagctcgcg
     1441 caccaggagc tcgcgcaggt tcttctcgtc gtcggccacc agcacccgga tcggcgtggt
     1501 cacggctttc ccttccccgc ctcggcgggt tggagcggca ggatgacccg gaaggtggtc
     1561 cccgcgccca cccgggtgtc cacctcgatc cgtccctcgt ggtcggccac gatgccctgg
     1621 gcgatggcga ggccgagccc ggtgccctgc ccgggcggct tggtggtgaa gaatgggtcg
     1681 aagacccgcg gcagcacgtg ctccggaatg ccgggtccct gatcctcgaa gcggatctcc
     1741 acctcgcgcg cgttgcgccg tcgagtcgtc acggtcagcg tgccctttcc gttcatcgcg
     1801 tcgagcgcat tgctcgcgag tccgaggaag acctggcgga gctggccctc attggccacc
     1861 acgtcgggca gctccgcgtc cggccggcgc tcgagccggc tctgcgcgta ccgcgactgg
     1921 tggccgatca gctccagcac cttgtcgacc agcacgttca ggtcggtggg agcccggcgg
     1981 ctgcccggat cccgcacgaa gtggagcagg ctcccggtga tttccttgca ccggtaggcc
     2041 tcctcctcga tcagggtcag atagccgggg aaatccttga acgcgtccag atctcgaaga
     2101 tgcgggtcct gggcgcgctc ccggagcgcc tccgcgcagc ccgcgatggt ggcgagcggg
     2161 ttgttcagct cgtgggccac gccggcggcc agccgccccg cggtggtgag ccgctcggtc
     2221 agcagcatct gccgctcgag ccgcttctgg agcgtgatgt cctccaccag caccagcacg
     2281 tgggtggcct gcggagcccg cagcggggtc gcggtcaagc gcacggtgag cgtctcgccg
     2341 cccgcgggca gctcctgctc cgcgtgctgg acctcgcggg ccgccaccac gccctccagg
     2401 aaggtgcgca ccgcctccgc cttggccgcc ggcatcagct ccgcgaacgg gcgccgcgcg
     2461 gtcggcccca ccggcaccag cgcggtgccc tcgcggttgg cgctgatcac gcgcagctcc
     2521 gcgtcgagca cgaagaggcc cagcggcagg gtctcgagca ccacctccac gaagcgcttc
     2581 tgctcgtcca gctcgtgggt gcgcgcctcc accatggcct cgagtcgctc ggagtaggtg
     2641 cggaccgagc tgaagagacg ggcgtgctcg agcgccatcg cggcctggtc ggcgaaggcc
     2701 gacacgagcg actcctcctc ggcgctgaag tggtgcacgt cggagcgcag gacggtgagg
     2761 gccccgatcg cctggtcgcc cacgatgagc ggggccgcca gcatcgagcg caggccggac
     2821 tgcgggtagc gcactcgcgg atcgcggtag aga
//
LOCUS       DATMCV010000551         2353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_897653, whole genome shotgun sequence.
ACCESSION   DATMCV010000551 DATMCV010000000
VERSION     DATMCV010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897653"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VNU03_12665"
     CDS             <1..937
                     /locus_tag="VNU03_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWP75365.1"
                     /translation="LLAPGVAGSRRRRALLGVLLLTVAAVSAWPWLALTDRTLRVTFL
                     DVGQGDAILIELPDGPRVLVDGGPGGPRRLDVGERVLAPYLWNRPIGHLDVVALSHWD
                     VDHSGGLAAVFSRFRVGEFWESGRAPAGALETVTALTRARVPRRALAAGQRIWLGHAL
                     LTVLGPAPGPPLHANDESLVLRLDWRGVSLLLPGDLGARGEAILLERGGPLRTLALKV
                     AHHGSRSSTGAPFLEAARPRIAVISVGARNPFRHPSPEAVARLEASGARVYRTDRDGA
                     VILETDGTVVRVTAWARRATDTFHLDDPENTAAPG"
     gene            complement(978..1976)
                     /locus_tag="VNU03_12670"
     CDS             complement(978..1976)
                     /locus_tag="VNU03_12670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWP75366.1"
                     /translation="MQELLLPVRHEWLARERGYGKIVIEPFEPGFALTAGNAYRRVLL
                     SSISGSAPTWVKIEGVLHEFSHLVGVREDTLDIILNLRKLVFTLHVNRPKLIRLKAQG
                     VRTVTARDFEPDPDVEILTPDVALATLDKDGSLEMEVCVERGRGYQPAEKREPEALPI
                     NAMLVDADFSPVKRVNFHVESQPSGQERLVMELWTNGTVTPEIAIGDASRILDDQFAL
                     LIDFPVAPQEEAEGTEQGTEPAPRSEVNEHLFRNVDELELSVRASNCLKTANIRTIAD
                     LVQKTESELLKTKNFGKKSLNEIKTILGEMGLSLGMRLDPEELERLRAHYERSFET"
     gene            complement(2049..>2353)
                     /locus_tag="VNU03_12675"
     CDS             complement(2049..>2353)
                     /locus_tag="VNU03_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75367.1"
                     /translation="YNLFHIASGLVGIALVLRAGGRGAVGFNLAFGLIDLYQAVAGSV
                     DLFPAALFAYQPLDDVLHVILGLGLVGLGALGWGRGMAGAPAGGDPARGGPGIGVV"
BASE COUNT          306 a          853 c          838 g          356 t
ORIGIN      
        1 gctgctggcg cccggggtgg ccggatcgcg ccgtcgccgg gcgctgctgg gcgtcctgct
       61 gctcaccgtc gccgcagtat cggcatggcc ctggctcgct ctcaccgacc gcacgctccg
      121 ggtgaccttc ctcgacgtgg gccagggcga cgcgatcctg atcgagctgc ccgacgggcc
      181 gcgcgtgctg gtggacggcg gcccgggcgg ccctcgccgg ctcgacgtgg gcgagcgcgt
      241 gctggcgccg tatctctgga accggccgat cggtcacctg gacgtcgtcg ccctctccca
      301 ctgggacgtc gatcactcgg gcggcctggc cgcggtgttc tcgcgcttcc gggtgggcga
      361 gttctgggag agcggccgcg cgcccgccgg ggcgctcgag acggtgacgg cgctgacccg
      421 cgcgcgggtg ccccgccgcg cgctcgccgc cggtcagcgg atctggctgg gacacgcgct
      481 cctcaccgtg ctggggccgg cgccggggcc gccgcttcac gccaacgacg agtcactcgt
      541 gctgcgcctc gactggcgcg gcgtgtccct gctgttgccg ggagacctgg gcgcgcgcgg
      601 cgaggcgatc ctgctggagc gcgggggccc cctccggacg ctcgcgctca aggtggccca
      661 ccacggcagc cgctcctcga ccggcgcgcc gtttctggag gcggcgcggc cgcggatcgc
      721 ggtgatctcg gtcggcgcgc gcaatccgtt ccgacatccg agcccggagg cggtggcccg
      781 gctcgaggcg tcaggggccc gcgtctaccg caccgatcgc gacggcgcgg tgatcctcga
      841 gaccgacggc accgtggtgc gggtgaccgc gtgggcccgc cgcgccaccg acacgtttca
      901 cctggacgac cccgaaaaca ccgcggcccc cgggtgaccg gaggccgtgg tgggacgcgc
      961 gggtagcggg agggagacta cgtctcgaag ctgcgctcgt agtgcgcgcg cagccgctcc
     1021 agctcctcgg gatcgagacg catgccgagg gagagcccca tctcgcccag gatggtcttg
     1081 atctcgttca gcgacttctt gccgaagttc ttggtcttga gcagctccga ctccgtcttc
     1141 tgcacgaggt cggcgatggt ccggatgttg gcggtcttca ggcagttcga cgcgcgtacg
     1201 gacagctcga gctcgtccac gttgcggaag aggtgctcgt tcacctcgct gcgcggcgcc
     1261 ggctcggtgc cctgctcggt gccctcggcc tcttcctgcg gcgccaccgg gaagtcgatc
     1321 aggagcgcga actggtcgtc caggatccgg gaggcgtccc cgatcgcgat ctcgggcgtc
     1381 acggtgccgt tcgtccacag ctccatcacc agccgctcct gcccggaggg ctgactctcc
     1441 acgtggaagt tcacgcgctt caccggcgag aagtccgcgt cgaccagcat cgcgttgatg
     1501 ggcagcgcct ccggctcgcg cttctcggcg ggctggtagc cgcgcccgcg ctcgacgcac
     1561 acttccatct cgaggctgcc gtccttgtcg agggtggcga gcgccacgtc gggggtcagg
     1621 atctccacgt cgggatccgg ctcgaagtcc cgcgcggtca ccgtccgcac cccctgcgcc
     1681 ttcaggcgaa tcagcttcgg ccgattcacg tgcagcgtga acacgagctt gcgcaggttc
     1741 aggatgatgt ccagggtgtc ctcgcgcacg ccgaccaggt gcgagaactc gtgcagaacc
     1801 ccctcgatct tgacccacgt gggcgccgat ccggagatcg aggacagcag gacgcgccgg
     1861 taggcgttcc ccgcggtcag ggcgaagccc ggctcgaacg gctcgatgac gatcttcccg
     1921 tagccacgct cacgggcgag ccactcgtga cggactggaa gcagcagctc ctgcattggg
     1981 tctcctcctc ggggtgtcag ggggggtcgc ccccaggccg gtgattatac cagaagcccg
     2041 cggggagcct agacgacccc gatcccgggg ccgccacggg cggggtcacc cccggccggc
     2101 gctcccgcca tcccccggcc ccagccgagc gcccccaggc ccaccaggcc cagccccagg
     2161 atcacgtgca ggacgtcgtc gaggggctgg taggcgaaca gggcggccgg gaacaggtct
     2221 accgagccgg ccacggcctg gtagaggtcg atcagcccga acgccaggtt gaatccgacc
     2281 gctccccggc ccccggcccg gaggaccagg gcgattccga ccagcccgct cgcgatgtgg
     2341 aagaggttgt acg
//
LOCUS       DATMCV010000552         4438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_431927, whole genome shotgun sequence.
ACCESSION   DATMCV010000552 DATMCV010000000
VERSION     DATMCV010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431927"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNU03_12680"
     CDS             <1..210
                     /locus_tag="VNU03_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP75368.1"
                     /translation="DTVYACGDVCAHRGGPLGEGKLTGPRLACPWHGWMYDVRTGQCV
                     FPGRGTAIPSYPVRLEADQVLVELP"
     gene            207..656
                     /locus_tag="VNU03_12685"
     CDS             207..656
                     /locus_tag="VNU03_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWP75369.1"
                     /translation="MITVKRLTLEDALVLLAAAEAKAREIAVTETICVCDDGGHPIAL
                     HRMTGARITGVEIAIAKAFTAAGHRRATHRFNQPPGGPALPGNEAFGIHAMHPGRFAV
                     FVGGFPIEVDGDVVGGIGVSGGNGEQDTLVGTAALDAFLRHLSATSR"
     gene            721..2628
                     /locus_tag="VNU03_12690"
     CDS             721..2628
                     /locus_tag="VNU03_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP75370.1"
                     /translation="MSLPASAVDAEGPALPARSQLEPSLLTRVAAWALPVEIALVTVA
                     IFLPALRAAWVDWDDPINFLLNPHYRGLGWAQLRWMVTANVMGHWIPTTWLTLGADYA
                     LWGMKPFGYHLTSLLLHAASAAVFYLVARRLLALAVPAASPLAVSAGATAAALFFAIH
                     PLRVESVAWITERRDVTSGLFFLLTIFAYLRAHERPPAVRSGWRWAALAFAALALTSK
                     SIVMGLPLVLVILDVYPLRRLGPRLRDWVAAAAWPIWREKIPFVLLAAATGAVAFLVQ
                     RSTGYLTDAEPAARVVMVFQNVWFHAWKTFVPLDLAPIYELPSRLSLLEKPYLLSAAG
                     SVATTVAVWLLRKRWPAGLATWAFYLVMLAPVSGIVHTGNHLGADRNTYLPCAGFAVL
                     VGALVVGAAEARRRNWLRTPVAALVVALVVVWIVGLGFATRIQTGVWHDSETLWRYAL
                     QVESTCSICNHNLAVILGQRGDQAQALALFERALALRPDRSDFYSSYGVLLLQMGRRP
                     DGLAALRYRISRAPGDFSARTNLGIALIEDGHLAAATAQLEQALRIKPDSVPALDALG
                     RALLTEDRVDAAMAAFERALAVNPTDSVGRLGMARVYLARGDRAAARAQFEILRARDP
                     NLARRVEQEFR"
     gene            2662..3438
                     /locus_tag="VNU03_12695"
     CDS             2662..3438
                     /locus_tag="VNU03_12695"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75371.1"
                     /translation="MATEAISVQHLSKLYATRDGGVAALERISFAIDEGAFVAVVGPS
                     GCGKSTLLKILAGLLAPSGGSASLRGTTIQGPRRDIGVVFQAPVLFPWRTVLDNVLLP
                     IDVQGLGRDRFRPVAMDLLALVGLREFERRYPWELSGGMQQRVAITRALVHDPAMLLM
                     DEPFGALDAMTREHMNLELQRIWMEKKKTVFFITHSIPEAVFLADRVLVMTPRPGRIV
                     DDLVIKLPRPRPLDVMTTPEFGVYTRHIRSLFNVKGGLDA"
     gene            3435..4226
                     /locus_tag="VNU03_12700"
     CDS             3435..4226
                     /locus_tag="VNU03_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75372.1"
                     /translation="MRSTRNVALSVLLFCASVGAWEGLVRLFQVPAFILPPPSQVALA
                     LWRGFSSGLYVEHLSHTLLETMLGFIAGSALGLILGTAVAMSRPVEFFLYPYIVMFQS
                     LPKVALAPLIVVWFGLGLTSKVINAALIAFFPLLVNTMVGLRSADEERVSLMRSLAAS
                     ERQIFWMLRLPNSLPFVMAGLDVAMIFALIGAIVAEFVGAQKGLGMLIQSMNFNMDVS
                     GQFSILLILSVVGLLLNRGVMMIRKRVLFWDPSEKERIDQERVSA"
     gene            4251..>4438
                     /locus_tag="VNU03_12705"
     CDS             4251..>4438
                     /locus_tag="VNU03_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HWP75373.1"
                     /translation="MAPNGNPSRIGVDIGGTFTDLVWVDETTSAVRVGKLLTTPKDPS
                     QAVEEGVVTLLHEAGAGAG"
BASE COUNT          577 a         1597 c         1505 g          759 t
ORIGIN      
        1 gacacggtgt acgcgtgcgg cgacgtgtgc gcccaccggg gtggtcccct cggcgagggc
       61 aagctcaccg ggccgcggct cgcgtgccca tggcacggat ggatgtacga cgtgcggacc
      121 ggccagtgtg tctttccggg acgcggtacc gccatcccca gctacccggt ccgcctggag
      181 gccgaccagg tcctcgtgga gctgccgtga tcaccgtcaa gcggctcacg ctggaggacg
      241 ccctcgtgct cctggccgcc gcggaggcga aggcccgcga gatcgcggtg accgagacga
      301 tctgcgtgtg cgacgacggg ggccatccca tcgcgctcca ccggatgacc ggggcgcgca
      361 tcaccggggt ggagatcgcc atcgccaagg cgttcaccgc ggccggccac cgccgggcca
      421 cccaccggtt caaccagccg cccgggggtc cggcccttcc gggcaacgaa gccttcggca
      481 tccacgccat gcatccgggc cgcttcgcgg tgttcgtggg cggcttcccg atcgaggtcg
      541 acggggacgt ggtgggcggc atcggagtga gcggtggcaa cggggagcaa gacaccctgg
      601 tggggacggc ggccctcgac gccttcctgc gccacctgag cgcgacctcg cggtaactcc
      661 cggccggccg ggtcggcgtt ccttgaccgg ctcgcgcccg acctcgtaga ctggttggtg
      721 atgtcccttc ccgcctcggc agtggacgcc gaagggcccg cgcttcccgc cagatcccag
      781 ctcgagccct ccctgctcac ccgcgtcgcc gcctgggcgc tgcccgtcga gatcgccctg
      841 gtgacggtgg cgatcttcct gcccgctctc cgcgccgcct gggttgactg ggacgatccc
      901 atcaacttcc tcctgaaccc gcactaccgg ggtctcggat gggcgcagct ccgctggatg
      961 gtcaccgcga acgtgatggg acactggatc ccgacgacgt ggctgaccct cggcgccgac
     1021 tacgcgctgt gggggatgaa gccgttcggc tatcacctca cgagcctgct cttgcacgcg
     1081 gccagcgccg cggtgttcta cctcgtcgcc cgacgcctgc tcgcgctggc ggtgcccgcg
     1141 gcctcgccgc tcgccgtgag cgcgggcgcc accgcggccg cgctcttctt cgccatccat
     1201 ccgcttcgcg tggaatcggt ggcctggatc accgagcggc gggatgtcac gtcggggctg
     1261 ttcttcctcc tcacgatttt cgcgtacctc cgggcccacg agcggcctcc cgcggtacga
     1321 tcgggctggc ggtgggccgc gctggccttc gccgcgctgg ccctgacctc gaagtccatc
     1381 gtgatgggcc tgcccctggt cctcgtgatc ctcgacgtgt atccgctgcg ccggctcgga
     1441 ccccgtctcc gcgactgggt ggccgccgcc gcgtggccga tctggcgcga gaagattcca
     1501 ttcgtgctgc tggcggccgc caccggcgcg gtggcgttcc tcgtccagcg gtccaccgga
     1561 tatctcaccg atgccgagcc ggcggcccgg gtggtgatgg tcttccagaa cgtctggttc
     1621 cacgcctgga agaccttcgt gccgctcgac ctggccccga tctacgagct gccgagccgg
     1681 ctgagcctgc tcgagaagcc gtatctcctg agcgcggcgg gatcggtggc gacgaccgtc
     1741 gcggtctggc tcctgcgaaa gcgatggccg gccggtctcg cgacctgggc cttctatctc
     1801 gtgatgctgg cgccggtgag cgggatcgtc cacaccggca accatctcgg agccgaccgc
     1861 aacacctact tgccgtgtgc cgggttcgcg gtcctggtcg gggccctcgt ggtcggggcc
     1921 gcggaggccc gccgccggaa ctggctccgc acaccggtcg cggcgctcgt ggtcgcgctc
     1981 gtcgtcgtct ggatcgtcgg cctcggcttc gcgacccgga tccagaccgg ggtgtggcac
     2041 gactcggaga cgctgtggcg ctacgccctc caggtcgagt cgacctgctc catctgcaac
     2101 cacaatctgg cggtcatcct ggggcagcgc ggggatcaag cccaggccct cgccctcttc
     2161 gagcgcgctc tcgcgctgcg acccgaccga tccgacttct acagcagcta cggggtcctg
     2221 ctgctccaga tgggccggcg tccggacggc ctcgccgcgc tccgctaccg gatctcccgg
     2281 gccccgggcg acttcagcgc gcgcaccaac ctgggcatcg ccctgatcga ggatggacac
     2341 ctcgccgccg cgaccgcgca gctcgagcag gcccttcgga taaaaccgga ctcggtgccc
     2401 gccctggacg cgctcggacg agccctgctc accgaggatc gggtggacgc ggcgatggcc
     2461 gcgttcgagc gggccctcgc ggtgaatccg accgactcgg tgggccgcct cgggatggcc
     2521 cgggtctacc tggcgcgcgg cgatcgcgcg gccgcgcgcg cgcagttcga gatcctgcgc
     2581 gcgcgggatc cgaacctcgc gcgccgcgtg gagcaggagt ttcggtagag cccgtccgtt
     2641 tcggtgtaga ctcccagcga agtggccacg gaagcgatct cggtccagca cctgtccaag
     2701 ctctatgcca cgcgggacgg cggggtcgcc gccctcgagc gaatcagctt cgccatcgac
     2761 gagggcgcgt tcgtggcggt ggtggggccg tcgggctgtg gcaagtccac cttgctcaag
     2821 atcctggccg gcctgctggc gccctcgggc ggaagcgcct cgcttcgcgg caccaccatc
     2881 cagggcccgc ggcgcgacat cggcgtggtc ttccaggcgc cggtcctctt cccgtggcgc
     2941 accgtcctcg acaacgtgct cctgcccatc gacgtgcagg gcctcggccg ggaccgattc
     3001 cggccggtgg cgatggacct gctggccctg gtcggcctcc gcgagttcga gcgccgctat
     3061 ccgtgggagc tgtccggcgg gatgcagcag cgggtggcga tcacacgcgc gctcgtccac
     3121 gatccggcca tgctcttgat ggacgagcca ttcggcgccc tcgacgccat gacccgcgag
     3181 cacatgaacc tggagctgca gcgcatctgg atggagaaga agaagaccgt cttcttcatc
     3241 acccactcga tcccggaggc ggtcttcctc gccgaccggg tcctggtcat gacgccgcgg
     3301 ccgggccgga tcgtcgacga tctggtgatc aagctcccgc ggccccggcc cctagacgtc
     3361 atgacgactc cggagttcgg ggtctacacc cgccacatcc ggtcactgtt caacgtgaag
     3421 gggggcctcg acgcgtgagg agcacgagga acgtcgcgct gtccgtcttg ctgttctgcg
     3481 cctcggtcgg cgcctgggaa gggctggtgc ggctgttcca ggtgcccgcc ttcatcctgc
     3541 ctccgccgtc ccaggtcgcg ctggccctct ggcgcgggtt cagcagcgga ctctacgtcg
     3601 agcacctctc gcacaccctg ctcgagacga tgctcggctt catcgcgggc tccgcgctgg
     3661 gcctgatcct cggcaccgcg gtggccatga gccgcccggt cgagttcttc ctctatcctt
     3721 acatcgtcat gttccagtcg ctccccaagg tggccctggc ccccctgatc gtggtctggt
     3781 tcgggctcgg cctcacctcg aaggtgatca acgcggccct catcgcgttc tttccgctcc
     3841 tggtgaacac catggtgggg ctgcgctccg ccgacgagga gcgggtgagc ctgatgcgct
     3901 cgctcgcggc gagcgagcgg cagatcttct ggatgctgcg cctgccgaac tccctgccct
     3961 tcgtgatggc cgggctcgac gtggcgatga tcttcgccct gatcggggca atcgtggccg
     4021 agttcgtcgg agcccagaag ggcctcggca tgctgatcca gtccatgaac ttcaacatgg
     4081 acgtctccgg gcagttctcc atcctgctga tcctgtcggt ggtggggctg ctgctcaacc
     4141 gcggggtcat gatgatccgc aagcgcgtgc tcttctggga tccgtccgag aaggagcgca
     4201 tcgaccagga aagggtcagc gcgtgacgcg cgcccccgag aggtgaaacc atggctccga
     4261 atggcaaccc gtcccgtatc ggcgtcgaca tcggcggtac cttcacggat ctggtctggg
     4321 tcgacgagac caccagcgcc gttcgcgtcg gcaagctcct gaccaccccc aaggacccgt
     4381 cccaggccgt ggaggagggc gtggtcacgc tcctccacga ggcgggcgcg ggagcggg
//
LOCUS       DATMCV010000553         2859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_496764, whole genome shotgun sequence.
ACCESSION   DATMCV010000553 DATMCV010000000
VERSION     DATMCV010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496764"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..567)
                     /locus_tag="VNU03_12710"
     CDS             complement(28..567)
                     /locus_tag="VNU03_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWP75374.1"
                     /translation="MAQTPATSAQAQEAQLDPRLVLDEMKKNGVSHVVWLPDSETNFL
                     YQLMQADPTLTLVPVSREGLAFSIAAGLATGGQRPIILIQNTGLMESGDSLRGWAIGL
                     QIPVVMMVGYRGWTRHGVNTDSAATYTERFLNAFGITYYLVETSADAPRISIAFEEAH
                     RTQRPVAVLMADEYHGFNR"
     gene            complement(570..2639)
                     /locus_tag="VNU03_12715"
     CDS             complement(570..2639)
                     /locus_tag="VNU03_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP75375.1"
                     /translation="MPQTKLVGVDVGGTFTDLVLVDEATGLVRVAKVPTTVANQAGGV
                     QAALAGAAAGAAEVAVIVHGTTTATNALLERKGARTGLITTRGFRDVLELGRRTRPTP
                     YGLKGSFEPLIPRDLRLEVDERVDAEGLVVTPLAPDQMREAVLRLREQGVEALVIHFM
                     HSYVNDAHERQAREIAAALWPNAYITVGSELLPEYREFERGTTAAINGFVQPVIDRYL
                     RTLAAELARQGYRRELLIMQGNGGTMSVDLAARHAVSTLMSGPAAGVKAAAYTALAAG
                     HRNIITCDMGGTSFDVGVIRDGRPALTADKEMGYGLPVRVPMIDIHTIGAGGGSIARV
                     NSAGILQVGPESAGADPGSICYGRGGEEPTITDANLLLGRLNPAGLLGVEQAARLDRI
                     REIFDKKIGAHLGLGPDEVASAIVRVANDKMAGAIRLVSLQRGHDPRDFVLFAFGGAG
                     PLHAVALARELAIPRVLVPARPGITSALGCLVADVRHDFVKTVNQGVLRLDLAEAHRI
                     LAAQVDEGRRMLASEGVEVETVSVQHEADMQFVGQTHVLTVPVARTDFAREDLMGAFE
                     AAYWERFEVELREMRAQVVSLRTAVIGRRRPVSLQGLITLEPAAGEVTPAARRRAWFD
                     GQWQDTPVYRREQLGVGAELVGPAIVEQLDSTTVIEPGDRVRVDALGNLEITVRGLET
                     ATLPETH"
BASE COUNT          379 a         1028 c         1033 g          419 t
ORIGIN      
        1 cggcggtctg gaacaagggg cggcctccta gcggttgaac ccgtggtact cgtcggccat
       61 cagcaccgcg accggccgct gggtgcggtg cgcctcctcg aaggcgatgg agatgcgcgg
      121 ggcgtcggcc gaggtctcga ccaggtaata ggtgatgccg aaggcgttga ggaagcgctc
      181 ggtgtaggtc gccgcgctgt ccgtgttcac cccgtggcgg gtccacccgc ggtagcccac
      241 catcatcacc accgggatct gcagcccgat cgcccagccc cgcagcgaat cgcccgactc
      301 catgaggccc gtgttctgga tcaggatgat cgggcgctgg cccccggtcg cgagcccggc
      361 cgcgatcgag aacgcgagcc cctcccggct gaccggcacg agcgtgagcg tcggatcggc
      421 ctgcatgagc tggtagagga agttggtctc gctgtcgggg agccacacta catgactgac
      481 cccgttcttc ttcatctcgt cgagcacgag ccgggggtcg agctgggctt cttgagcctg
      541 ggctgaggtc gcgggggtct gggccatggt caatgggtct ccggtagcgt cgcggtctcc
      601 aggccgcgga cggtgatctc gaggttgccg agcgcgtcca cgcgcacccg gtcccccggc
      661 tcgatcacgg tggtggaatc gagctgctcg acgatggccg ggcccacgag ctccgccccg
      721 acccccagct gctcccgtcg gtacaccggc gtgtcctgcc actgcccgtc gaaccaggcg
      781 cgccggcgcg cggccggcgt cacctcgccc gccgccggct ccagagtgat caagccctgg
      841 agcgacaccg gccggcgccg gccgatcacc gcggtgcgca ggctcacgac ctgggcgcgc
      901 atctcgcgca gctccacctc gaagcgctcc cagtaggcgg cctcgaaggc gcccatcagg
      961 tcctcgcgcg cgaagtcggt ccgcgcgacc ggcaccgtca gcacgtgggt ctgcccgacg
     1021 aactgcatgt ctgcctcgtg ctggacgctg accgtctcga cctccacccc ttccgatgcc
     1081 agcatccggc ggccctcgtc cacctgggcc gcgaggatgc ggtgcgcctc ggccaggtcc
     1141 agccgcagca cgccctggtt gaccgtcttc acgaagtcgt ggcgcacgtc ggccaccagg
     1201 cagccgagcg ccgaggtgat gccgggccgg gccggcacca gcacccgcgg gatcgcgagc
     1261 tcgcgggcca gggcgaccgc gtgcagcggg cccgcgccgc cgaaggcgaa gagcacgaag
     1321 tcgcgcgggt cgtgaccgcg ctggagcgac accaggcgga tggccccggc catcttgtcg
     1381 ttggccaccc gcacgatggc gctggccacc tcgtcgggtc cgaggcccag gtgcgcgccg
     1441 atcttcttgt cgaagatctc gcggatgcga tcgagccggg cggcctgctc gacgccgagc
     1501 aacccggcgg gattgaggcg gcccagcagc aagttggcgt cggtgatggt cggctcctcg
     1561 cccccgcgcc cgtaacagat cgacccggga tcggcccccg cgctctcggg cccgacctgc
     1621 aggatgccgg ccgagttcac gcgggcgatg gagccgccgc cggcgccgat ggtgtggatg
     1681 tcgatcatcg gcacgcggac cggcagcccg tagcccatct ccttgtccgc ggtcagcgcg
     1741 ggccgcccgt cgcggatgac gcccacgtcg aagctggtcc cgcccatgtc gcacgtgatg
     1801 atgttgcggt gcccggccgc cagcgcggtg taggcggcgg ccttcacccc cgccgccgga
     1861 cccgacatga gcgtgctcac cgcgtggcgc gcggccaggt ccaccgacat ggtgccgccg
     1921 ttgccctgca tgatcagcag ctcgcgccgg tagccctgtc gcgccagctc ggcggccagc
     1981 gtgcgcaggt accggtcgat gaccggctgc acgaacccgt tgatggccgc ggtcgtgccg
     2041 cgctcgaact cgcggtactc gggcaggagc tcggagccca ccgtgatgta ggcgttgggc
     2101 cagagcgcgg cggcgatctc gcgcgcctgg cgctcgtgcg cgtcgttgac gtacgagtgc
     2161 atgaagtgga tcaccagggc ctcgacgccc tgttcgcgca ggcgcaggac cgcctcgcgc
     2221 atctggtccg gagcgagcgg cgtgaccacc agcccctcgg cgtcgacccg ctcgtccacc
     2281 tcgaggcgga gatcgcgcgg gatcagcggc tcgaagctgc ccttgagccc gtagggcgtg
     2341 gggcgcgtgc gccggcccag ctcgagcacg tcgcggaagc cgcgcgtggt gatgagcccg
     2401 gtgcgggcac ccttgcgctc gagcagcgcg ttggtcgcgg tggtcgtccc gtggacgatg
     2461 accgctacct ccgccgcgcc cgccgcggcg ccggccaggg ctgcctgcac gccgccggcc
     2521 tggttggcca cggtggtcgg gaccttggcc acccggacca ggccggtggc ctcgtcgacg
     2581 agcaccaggt cggtgaacgt gccgccgacg tcgacgccga cgagcttggt ctggggcatc
     2641 tatcccaggg gcgagcgtgg gcagtcagga gacatgggtc acgggtctcg gcgttgggat
     2701 gagcgagagc ctagtcgccg ggccgggccc tgtcaagcgc gccggctagg gctgcctctc
     2761 gcgcgcgtcc ggcccggccg cgagcacgcg gtcgaggtag ccgttcaggt agtgatggag
     2821 gggcttctcc tgcgtcgaca tgcgccccgg tcggtacgc
//
LOCUS       DATMCV010000554         3797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_297010, whole genome shotgun sequence.
ACCESSION   DATMCV010000554 DATMCV010000000
VERSION     DATMCV010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297010"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VNU03_12720"
     CDS             <1..538
                     /locus_tag="VNU03_12720"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75376.1"
                     /translation="AVDEVNLPLHFHTFPSMPPGVREKATGLTRRAAMFTGVSAFQMN
                     LVNILAAIIGAAVLERYPNVRIAFGESGIGWVPYALDRMDFEWEDRFTDLGLKMKPSD
                     YWRRQCKATFQFDRIGTKLIDDMGVETLMWGSDYPHGDGVWPDSSKYIAEQFGHLPAD
                     VMHKMTCENAGRFYGLIK"
     gene            complement(598..1659)
                     /locus_tag="VNU03_12725"
     CDS             complement(598..1659)
                     /locus_tag="VNU03_12725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP75377.1"
                     /translation="MAPEQATLDPKKVDEFLGKAISDVAATFHAGLVLIGDKLGLYRA
                     MAGAGPMSPADLAKRTGARERYVREWLSAQAAGGYVTYEPATGRFTLPPEQAFLLLDA
                     DLPGAFQLSIGSVRDEPKISDAFRTGAGVGWHEHDAGVYEGCERFFRPGYAMNLVSQW
                     IPALEGVKARLEVGGQVADVGCGHGASSIIMAQAFPRATITGFDYHPASIQAARQAAD
                     KAGVADRLSFEVIAAKTYPGTGYDLVTFFDCLHDMGDPVGAARHVRQSLAPGGVWMLV
                     EPFANDRLEDNLNPVGRLYYAASTLLCTPASLSQEVGLALGAQAGEKRLREVLTEAGF
                     TRIRRVAQTPANMVLEVRA"
     gene            complement(1689..2495)
                     /locus_tag="VNU03_12730"
     CDS             complement(1689..2495)
                     /locus_tag="VNU03_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP75378.1"
                     /translation="MATMAPARAYVREAGVGPAVVCIHASASSSAQWRPLMDRLVSRY
                     RTLAVDLYGCGRSPRWPDDRALSLADEVALLEPVLAEAGDRFHVIGHSYGGAVALKAA
                     LADPGRLRSLVVYEPVLFSVLLAEDATQPAAREIVAVRDDTVAAMEGGNPRASGARFL
                     DYWMGAGTWAGMPEPRREAVATAMGSVAAEWHAAFTEPTPLSAFASLDVPTLCLTGSD
                     SPGASREVTRRLTKVLPRVTEVEIAGVGHMGPVTHPERINALIERHLETN"
     gene            complement(2498..2971)
                     /locus_tag="VNU03_12735"
     CDS             complement(2498..2971)
                     /locus_tag="VNU03_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP75379.1"
                     /translation="MRRIVPAVLMLSALVALVAIPLQADEHAHTVALPATLKWAEPAV
                     LPGALLAVVQGDPSKEGLFVYRLKMPAGYRIPPHLHKASENVTVLSGEFFIGLGEKLD
                     QGKGQELPVGAFLSVPPNHPHYAWVGRSETIVQVHGVGPTDPRFVNPEDDPRKKK"
     gene            3441..>3797
                     /locus_tag="VNU03_12740"
     CDS             3441..>3797
                     /locus_tag="VNU03_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWP75380.1"
                     /translation="MMIPTDPIVGVDVGASTISAGLVDRDGTVLTAAQAPTQGGSAVV
                     DTIFTLIDHALASAADRGLAVAGIGVGLPGLVDVEKGFMRSMPFAWLSELGDVPLAAL
                     VQERTGHPVFVDNDVNA"
BASE COUNT          568 a         1342 c         1328 g          559 t
ORIGIN      
        1 ggcggtcgac gaggtgaatc tgccgctcca cttccacacg ttcccctcga tgccgccggg
       61 tgttcgcgag aaggcgaccg ggctcacgcg ccgggccgcg atgttcacgg gcgtgtccgc
      121 cttccagatg aacctggtca acatcctggc cgcgatcatc ggggccgcgg tgctcgagcg
      181 ctatccgaac gtgcgcatcg ccttcggcga gtccggcatc ggctgggtgc cctacgcgct
      241 cgaccgcatg gacttcgagt gggaggaccg cttcaccgac ctcgggctca agatgaagcc
      301 gagcgactac tggcggcggc agtgcaaggc cacgttccag ttcgaccgca tcggcaccaa
      361 gctgatcgac gacatgggcg tggagacgct gatgtggggc tccgactacc cgcacggcga
      421 cggggtgtgg cccgactcca gcaagtacat cgccgagcag ttcggccacc tgccggccga
      481 cgtcatgcac aagatgacct gcgagaacgc gggccgcttc tacggcctga tcaagtagtc
      541 ccggggtcag gtcttacatt acgacaatgt cgtagtgtaa gacctgaccc caacctgtta
      601 cgcgcggact tcgaggacca tgttggcggg ggtctgcgcc acccgccgga tccgcgtgaa
      661 gcccgcctcg gtcagcacct cgcgcagccg cttctcaccc gcctgggcgc ccagcgcgag
      721 ccccacttcc tggctcagcg acgcgggcgt gcagagcagt gtcgacgccg cgtagtagag
      781 gcgcccgacc gggttgaggt tgtcctccag ccggtcgttg gcgaagggct ccacgagcat
      841 ccaaactccg cccggagcca gcgactgacg cacgtgccgc gccgctccca ccggatcgcc
      901 catgtcgtgc aggcaatcga agaaggtgac gagatcgtag ccggtgccgg ggtaggtctt
      961 ggccgcgatc acctcgaagg acaaccggtc cgccaccccg gccttgtcgg ccgcctgccg
     1021 cgcagcctgg atggaggcag ggtggtagtc gaagccggtg atggtcgcgc gggggaaggc
     1081 ctgcgccatg atgatgctgg acgcgccgtg gccgcagccc acatcggcga cctgcccgcc
     1141 gacctcgagc cgcgccttca ccccctcgag ggcggggatc cactggctga ccagattcat
     1201 ggcgtagccc ggacggaaga accgctcgca gccctcgtac acccccgcat cgtgctcgtg
     1261 ccagcccact ccagcgccgg tgcggaacgc atccgagatc ttcggctcgt cgcggaccga
     1321 gccgatgctc agctggaacg cccccggcag gtccgcgtcg agcagcagga acgcctgctc
     1381 cgggggcagc gtgaaacggc cggtggccgg ctcgtaggtc acgtacccgc ccgcggcctg
     1441 cgcggagagc cactcgcgca cgtagcgctc gcgcgcgccg gtacgcttgg ccaggtccgc
     1501 ggggctcatc gggccggcgc cggccatcgc ccggtacagg ccgagcttgt cgccgatcag
     1561 cacgaggccc gcgtggaacg tggccgcgac gtccgagatc gccttgccga ggaactcgtc
     1621 caccttcttc gggtccagcg ttgcctgctc tggtgccatg atgtttctcc tctgggcgga
     1681 tcgcccgtct agttggtttc gagatggcgc tcgatcagcg cgttgatccg ctccggatgg
     1741 gtgacggggc ccatgtgccc caccccggcg atctcgacct cggtcacgcg cggcaagacc
     1801 ttcgtcagcc gccgggtcac ctcccgggag gcaccgggag aatccgagcc ggtgaggcag
     1861 agggtcggca cgtcgagcga cgcgaacgcc gacagcggcg tcggctccgt gaacgccgcg
     1921 tgccactctg cggccacgct gcccatcgcc gtcgccaccg cctcgcgccg aggctccggc
     1981 atgccggccc aggtgccggc ccccatccag tagtcgagga accgggcccc ggacgcgcgc
     2041 gggttcccgc cctccatcgc ggcgacggtg tcgtcgcgca ccgccacgat ctcccgcgcc
     2101 gcgggctggg tggcatcctc ggccagcagg accgagaaca gcaccggctc gtagacgacg
     2161 agggagcgga gacgaccggg atcggcgagc gcggccttga gggccaccgc cccgccgtag
     2221 gagtggccga tcacgtggaa ccggtcgccc gcctccgcga ggaccggctc gagcaatgcc
     2281 acctcgtcgg ccagcgacag cgcccgatcg tccggccacc gcgggctccg accgcacccg
     2341 tagaggtcga ccgcgagcgt gcggtagcgg ctgaccagac ggtccatcaa gggccgccac
     2401 tgggccgacg agctggcgct cgcgtggatg cagacgacgg ccgggccgac cccggcctcg
     2461 cggacatacg ctcgagccgg tgccatcgtg gccatggcta cttcttcttc cgcggatcgt
     2521 cttccggatt cacgaaccgg ggatcggtcg gtcccacccc gtggacctgc acgatcgtct
     2581 cggagcggcc cacccacgcg tagtgcgggt ggttcggcgg cacggagagg aaggctccga
     2641 ccggcagctc ctgccccttg ccctggtcga gcttctcgcc gaggccgatg aagaactccc
     2701 cggagagcac cgtgacgttc tcgctggcct tgtgcaggtg cgggggaatg cggtagccgg
     2761 ccggcatctt gagtcgatac acgaacaacc cctccttgct cgggtcgccc tggacgaccg
     2821 cgagcagggc ccccggcaga accgcgggct cggcccactt gagggtcgcc ggcagggcga
     2881 cggtgtgcgc gtgctcgtcg gcttgcagtg ggatcgcgac gagcgcgacg agagcggaga
     2941 gcatcaggac cgcgggtacg atgcgacgca tggtcgatcc tcctgttgga tgggctgttg
     3001 actgggccgt tcgatcgggt gccatcgggt ccacgctgtg gagcaagcga cgtgccccgc
     3061 gggagcccgc gaaattccac gcggttgcgg gccggagccg caagttggcg ttgcggtgaa
     3121 gcggggagcg ccgattcggc cgcgcttgat ttcgccacgt tggcaccgca agctgacgtg
     3181 gcgcgacgag accatgcacg cgcaagagca gacggcaggg gaggtggggg aacgccgccg
     3241 cgcgggcgcg gagcatcgcg accttgcacg gtcggccggg cgcaccgcca tgtcaccttg
     3301 cggggctcgc gggcagtccc gacgggcgct tgccgagcaa ccggccccgc cccgcaagat
     3361 cgggttgcac cccacgcccc gcgagtggcc gtgctgagcg ggtttccgcg ctggcacacg
     3421 gcttgcggga gcgagtccgc atgatgatcc cgaccgatcc gatcgtcggc gtggacgtcg
     3481 gagcctcgac gatctcggcc gggctcgtcg accgcgacgg caccgtcctc actgccgccc
     3541 aggccccgac ccagggcggg agcgcggtgg tggacaccat cttcaccctg atcgatcacg
     3601 cgctcgcctc ggcggcggac cgcgggctcg cggtggccgg gatcggcgtg ggccttccgg
     3661 gcctcgtcga cgtcgagaag gggttcatgc gctcgatgcc gttcgcgtgg ctctcggagc
     3721 tcggcgacgt gccgctcgcg gctctcgtcc aggagcggac cggccacccc gtcttcgtgg
     3781 acaacgacgt gaacgcc
//
LOCUS       DATMCV010000555         5232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_561896, whole genome shotgun sequence.
ACCESSION   DATMCV010000555 DATMCV010000000
VERSION     DATMCV010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561896"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VNU03_12745"
     CDS             <1..427
                     /locus_tag="VNU03_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP75381.1"
                     /translation="HIRILKQGARMGQTESNLGIIPGFGGTQRYPRLMGRGRALEHLI
                     LGTQVPAEECYRTGLVNRLAKEGETLNDARALARELAKRPPIATRLIIEAVDDGMHAP
                     IDQAIEVEVRAFLQSLRTEDAGEGIQAFFQKRPADFKGR"
     gene            440..1189
                     /locus_tag="VNU03_12750"
     CDS             440..1189
                     /locus_tag="VNU03_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006666074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75382.1"
                     /translation="MDLGIAGKTALVTGGARSLGKQDALTLASEGCRVIVLDLNAEGA
                     AETAKEIADAGGTARGYAADITDRAVLAEVIGRAEREVGPVDICVNNAGWIYTMGQLK
                     DTADDDWDLNLRINLTGTYNVTKLVFPGMRERRWGRVICMASIAGLMGGFGQTAYSTS
                     KMGVIGFAKSVALEGARYNVTSNVIAPGIVGPNANMSPLYERMVKRVAMQREGQPEDI
                     ASAIAFLCSERARYITGAVLTVTGGMDLFTF"
     gene            1222..1542
                     /locus_tag="VNU03_12755"
     CDS             1222..1542
                     /locus_tag="VNU03_12755"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWP75383.1"
                     /translation="MPTVKETFDNMASRFRADKAAGVNATIQYDITGDQGGTWNAVIK
                     DGACNVTQGAATGPNLTLTMSSQDWLDMTGGKLSGQMAFMSGKLKLKGDMGLAMKVGS
                     LFQV"
     gene            1648..2748
                     /locus_tag="VNU03_12760"
     CDS             1648..2748
                     /locus_tag="VNU03_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP75384.1"
                     /translation="MRDELFTMMGEMPAMSPELLARMREEANRNLLRMKHWSEMVMNP
                     KEPEVGPTPRQLIYRTNKSRLWRYESKRTVRTPLLFVPNLGISRPYIFDLMPKGSFIE
                     YMTGQGFDFFLVDWGVFGEEDNGLTVEDAVTKILPRLAGKALEASGASEMSVLGYCMG
                     APLSASFVATHPEFPLKNYVDMAGPIDFAHVGLFGKWLDPRVFDVDKYVDTLGSIPAE
                     MVKMGFKLLKPTMDLNTNLNLWWNLWNPEYVAGFNALNKWANEYLPFPGEFFRHWVKD
                     FYQQNQLIKGQLMMAGRRVDLREIRCPVLAVGAKEDNIAPPACVKPLIDVVSTRDKEY
                     VELPGGHISLIAGRGASVHCWPKVASWLAARS"
     gene            2773..3306
                     /locus_tag="VNU03_12765"
     CDS             2773..3306
                     /locus_tag="VNU03_12765"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HWP75385.1"
                     /translation="MADNTSQQLLDMWKRQVEDGTQAWLRMMGQGPAAGTPPMDPQAF
                     WRPFMDQGMAAWSKVMTQGTAPSPDLMAQWKQFLDQWIAAWSRVLEQAMGTDTFAQMM
                     GKQLEGFLNVSGPAKKAAEQQIDASLAGLGLPSRAQVVALARHLAQVEEKIDSLEDKL
                     DRAVRRNTDATGRPERA"
     gene            3303..3872
                     /locus_tag="VNU03_12770"
     CDS             3303..3872
                     /locus_tag="VNU03_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP75386.1"
                     /translation="MIGMTIDAIAVGDSAQITRRVTDGDIASFVDAVGDYNPVHSDRA
                     YAAETVFKEPIAPGIWTAGLVSAVIGTRLPGPGAIYLSQDLKFLKPVKAGDSISARVE
                     VIDVNRERNRIRLRTVCTNHRAEDVLTGEAVVMPSRTPIDYARSTAPMGALALWTLQP
                     LAWMAQGATIVGMLGLSALSAASARPRSK"
     gene            3957..4460
                     /locus_tag="VNU03_12775"
     CDS             3957..4460
                     /locus_tag="VNU03_12775"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HWP75387.1"
                     /translation="MAYVIKRYSNRKLYDTQESRYVTLEELEELIRAGREISVVDVST
                     GEDLTSVTLAQIILENERNHRATLPTGFLHQLIKHGEAWQDFVQKSLRSSLEGLMTSQ
                     READRVLRDWASRAGWPNVASPGAEPVKPEKRADTAEADVLREEVSVLREQLRALEER
                     LEKKREK"
     gene            complement(4526..4975)
                     /locus_tag="VNU03_12780"
     CDS             complement(4526..4975)
                     /locus_tag="VNU03_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75388.1"
                     /translation="MTNSLQVNELMGELTGRTVEVFSLWADANQKVLRELVDLSAGTA
                     REGVRLYAEIQSAMLEAVKEGQAYLLSRQDAMQDAPRDPLGAYQKGALESAESAQKTF
                     RLLEGTAQATTRSAERLQVTAEHAGQEIQATVTQLAGKVQSLYAPLA"
BASE COUNT          885 a         1767 c         1815 g          765 t
ORIGIN      
        1 ccacatccgc atcctgaagc agggggcgcg gatggggcag accgagtcca acctcggcat
       61 catcccgggc ttcggcggca cgcagcgcta cccgcgcctc atggggcggg ggcgcgccct
      121 cgagcacctg atcctcggga cccaggtgcc cgcggaggag tgctaccgca ccgggctcgt
      181 caaccggctc gcgaaggaag gggagacgct caacgacgcg cgcgcgctcg cccgcgagct
      241 ggccaagcgc ccgccgatcg ccacgcgcct catcatcgag gcggtggacg acggcatgca
      301 cgcccccatc gaccaggcca tcgaggtcga ggtgcgggcc ttcctccagt cgctccgcac
      361 cgaggacgcg ggtgaaggca tccaggcctt cttccagaag cggcccgccg acttcaaggg
      421 ccgctaggtg gggcggtagg tggacctcgg catcgcgggc aagaccgcgc tggtgaccgg
      481 cggcgcccgg agcctcggca agcaggacgc cctcaccctg gcatcggaag gctgtcgcgt
      541 gatcgtgctg gatctcaacg cggagggcgc ggccgagacc gcgaaggaga tcgcggacgc
      601 gggcggcacc gcgcggggct acgcggctga catcaccgac cgcgcggtcc tggccgaggt
      661 gatcgggcgc gccgagcggg aggtcggtcc cgtcgacatc tgcgtcaaca acgcggggtg
      721 gatctacacg atgggccagc tcaaggacac cgcggacgac gactgggacc tgaacctccg
      781 catcaacctg accgggacct acaacgtcac gaagctggtg ttcccgggca tgcgcgagcg
      841 ccgctgggga cgcgtgatct gcatggcctc gatcgccgga ctgatgggcg gcttcgggca
      901 gaccgcgtac tcgacgagca agatgggggt gatcggcttc gccaagtcgg tcgcgctcga
      961 gggggcgcgg tacaatgtga cctcgaacgt gatcgctccc ggcatcgtgg ggccgaatgc
     1021 gaacatgtcg ccactgtacg agcgcatggt caagcgggtg gccatgcagc gggaaggaca
     1081 gcccgaagac atcgcgagcg cgatcgcgtt cctctgctcg gagcgcgcgc gctacatcac
     1141 gggggccgtg ctcaccgtca ccgggggcat ggacctcttc acgttctagg acacgctcga
     1201 caccggaaag gagcagtggt catgccgaca gtgaaggaga ccttcgacaa catggcatcc
     1261 cggttccgcg ccgacaaggc cgcgggggtg aacgccacca tccagtacga catcacgggg
     1321 gatcagggcg ggacctggaa cgcggtcatc aaggacggcg cctgcaacgt gacccagggc
     1381 gccgcgaccg gcccgaacct caccctcacc atgtcgtcgc aggactggct cgacatgacc
     1441 ggcggcaagc tgtccgggca gatggccttc atgtcgggca agctcaagct gaagggcgac
     1501 atgggcctcg ccatgaaggt ggggagcctc ttccaggtct agcccgtccc gcggccgcct
     1561 cccccggccc ggcgccccga ggggtcgggc cggggcgcgg cgcggatgac tgacgcgaag
     1621 cgtcacgccg ggtgtaggat aggagccatg agagacgagc tcttcacgat gatgggagag
     1681 atgccggcga tgtcgccgga gctgctggcg cgcatgcggg aagaggccaa ccgcaatctc
     1741 ctccgcatga agcactggag cgagatggtg atgaacccca aggagcccga ggtcgggccc
     1801 actccgcggc agctgatcta ccgcaccaac aagtcgcgcc tctggcggta cgagtcgaag
     1861 cgcacggtgc gcaccccgct gctcttcgtg cccaatctcg ggatcagccg gccctacatc
     1921 ttcgacctca tgcccaaggg ctcgttcatc gagtacatga ccggccaggg cttcgacttc
     1981 ttcctggtgg actggggcgt gttcggcgag gaggacaacg gcctcaccgt ggaggacgcg
     2041 gtcaccaaga tcctcccgcg gctcgccggc aaggcgctcg aggcctcggg cgcctccgag
     2101 atgtcggtgc tgggctactg catgggcgcc ccgctctccg cgagcttcgt ggcgacccat
     2161 ccggagttcc cgctcaagaa ctacgtcgac atggcgggcc ccatcgactt cgcccacgtg
     2221 gggctgttcg gcaagtggct cgacccgcgg gtcttcgacg tggacaagta cgtcgacacc
     2281 ctgggcagca tcccggccga gatggtcaag atgggcttca agctcctcaa gcccaccatg
     2341 gacctcaaca cgaacctgaa cctctggtgg aacctctgga accccgagta cgtggccggg
     2401 ttcaacgcgc tcaacaagtg ggccaacgag tacctgccat tcccgggaga gttcttccgt
     2461 cactgggtca aggacttcta ccagcagaac cagctcatca agggtcagct catgatggcg
     2521 ggtcgccggg tggacctgcg cgagatccgc tgcccggtgc tcgcggtggg ggccaaggaa
     2581 gacaacatcg cgccccccgc ctgcgtgaag ccgctcatcg acgtcgtgag cacgcgcgac
     2641 aaggagtacg tggagctgcc gggaggacac atctcgctga tcgccggacg cggcgcctcg
     2701 gtgcattgct ggccgaaggt ggcgtcctgg ctggccgcgc ggtcctagcc gcgggccggg
     2761 aaggacaacg tcatggccga caacaccagt cagcagctgc tcgacatgtg gaagcgccag
     2821 gtcgaggacg gcacccaggc ctggctccgg atgatggggc aggggccggc ggccgggaca
     2881 ccccccatgg atccgcaggc gttctggcgg cccttcatgg accagggcat ggccgcgtgg
     2941 tcgaaggtga tgacccaggg gaccgcgccg tcgcccgacc tgatggcgca gtggaagcag
     3001 ttcctcgacc agtggatcgc ggcgtggtcc cgggtcctgg agcaggcgat gggcaccgac
     3061 accttcgcgc agatgatggg caagcagctc gaaggcttcc tcaacgtctc cggacccgcc
     3121 aagaaggcgg ccgagcagca gatcgacgcc tcgctcgccg ggctcggcct gccgtcgcgc
     3181 gcccaggtcg tggcgctggc ccgccacctg gcgcaggtcg aggagaagat cgactccctc
     3241 gaggacaagc tggaccgcgc ggtccgccgg aacaccgacg caaccgggcg accggagcgc
     3301 gcatgatcgg catgacgatc gacgccatcg cggtgggcga cagcgcccag atcacccgcc
     3361 gcgtcaccga cggcgacatc gcctcgttcg tggacgcggt cggcgactac aacccggtgc
     3421 actcggaccg ggcgtacgcg gcggagaccg tgttcaagga gccgatcgcg ccggggatct
     3481 ggaccgcggg actcgtctcg gcggtgatcg gcacgcggct gcccgggccc ggcgccatct
     3541 acctctccca ggatctcaag ttcctcaagc cggtgaaggc gggcgattcg atctcggccc
     3601 gcgtcgaggt gatcgacgtc aaccgggaga ggaaccggat ccgcctgcgc accgtgtgta
     3661 ccaaccaccg ggccgaggac gtgctgaccg gcgaggcggt ggtgatgccg tcgcggaccc
     3721 cgatcgacta cgcgcgatcg accgcgccca tgggcgcgct cgcgctctgg acgctgcagc
     3781 cgctcgcgtg gatggcccag ggtgcgacca tcgtgggcat gctgggcctc tccgcgctct
     3841 ccgccgcgtc cgcgcgcccg cgctcgaaat agcgcgcgcc gtcgggcttc gcggcccgtc
     3901 gcggcggccg acttgccttg tcggggctgg accgcggtgc tagactgact tcgatcatgg
     3961 cgtacgtcat caagcgctac tccaatcgca agctctacga tacgcaggag agccgctacg
     4021 tcacgctgga ggagctcgag gagttgatcc gggccgggcg ggagatctcg gtcgtggatg
     4081 tctcgaccgg cgaggacctc acgtcggtca ccctcgcgca gatcatcctc gagaacgagc
     4141 ggaaccaccg cgcgacgctg cccaccgggt tcctgcacca gctcatcaag cacggagagg
     4201 cctggcagga cttcgtgcag aagtcgctcc gctcgagtct cgaagggctc atgacgagcc
     4261 agcgcgaagc cgaccgggtg ctgcgggact gggcttcgcg cgccgggtgg ccgaacgtcg
     4321 catcgcccgg cgccgagccg gtcaagcccg agaagcgggc cgacaccgcc gaggccgacg
     4381 tcctccgcga ggaggtctcg gtgctacgcg agcagctgcg cgcgctggaa gagcggctcg
     4441 agaagaagcg cgagaaatag cgacgcgggc cgggtcacgc gacccggccc gcgtcggcct
     4501 tccgtcggcc ccgcattcag atcattcagg cgagcggggc gtagagcgac tgcaccttgc
     4561 cggcgagctg ggtgaccgtg gcctggatct cctgaccggc gtgctcggcg gtgacctgca
     4621 gccgctccgc ggaccgggtc gtggcctgtg cggtaccctc gaggagccgg aaggtcttct
     4681 gcgcgctctc cgccgattcc agcgcaccct tctggtaggc cccgagcgga tcgcgcggcg
     4741 cgtcctgcat cgcgtcctgc cggctaagca ggtaggcctg cccctccttg accgcctcga
     4801 gcatggcgga ctggatctcg gcgtacaggc gcacgccctc ccgggcggtg ccggcggaaa
     4861 ggtccaccag ctcccggaga accttctggt tggcgtcggc ccacagcgaa aatacctcga
     4921 cggtgcgtcc ggtcagctcg cccatcagct cgttcacctg cagggaattg gtcatcggtt
     4981 cgctccttgg tgtcgtttct gtgatcgcac ggccgaggat aacgcggtgc gctatgagcg
     5041 tcaagcgttt tattgcgtcg cgtcacggcg cccgtgctat acgggtaacc tcttgaaact
     5101 catcgcgaac tggtgacccg agccatgtcc ctcactcgcc tcgcggacga gagcctcgac
     5161 aagtacggcg agtacgtcgc gctggcgttc gaggggcgcc ggtacaccaa tctggagcag
     5221 gcgcgggccg cc
//
LOCUS       DATMCV010000556         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_496926, whole genome shotgun sequence.
ACCESSION   DATMCV010000556 DATMCV010000000
VERSION     DATMCV010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496926"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VNU03_12785"
     CDS             <1..525
                     /locus_tag="VNU03_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75389.1"
                     /translation="SAGEGQGSVFTVKLPVAIARAGAVVDASPSAGRVHPTAPAGVPA
                     RGPSLGGLRVLVVDDDRDGLDLVATILMNSGAEVRACASVAEGFEALQAWRPDVLVSD
                     IEMPGEDGYALIRRVRGLASGPVARTPAVALTAYGRVEDRLRTLAAGYSMHVPKPVDP
                     AELATIVASLAGRP"
     gene            complement(534..1037)
                     /locus_tag="VNU03_12790"
     CDS             complement(534..1037)
                     /locus_tag="VNU03_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP75390.1"
                     /translation="MQFQLRKIGHVVLNVTDLTRAVTFYTDVLGLEVSDRYPDSMVPG
                     GMVFLRVNTDHHGVALVGGAPGSEHTSLNHFAFEVGSLDEVFRARAWLRERGVPIHFE
                     GRRRAGCQIAIEFRDPDGNNLEIYWNIDQVGTVGAARPADEWRPAKTLEEAVANPVKG
                     QTLPRRV"
     gene            complement(1052..1735)
                     /gene="msrA"
                     /locus_tag="VNU03_12795"
     CDS             complement(1052..1735)
                     /gene="msrA"
                     /locus_tag="VNU03_12795"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWP75391.1"
                     /translation="MIWLLVAVLLGALVGAPSSAPAAESAVVIPAPAVNDVKAAGPVQ
                     TAVLAGGCFWGVQGVYEHVRGVRQVLSGYAGGDRATATYEAVSAGRTRHAESVQIRFD
                     PKEISYGEILQIFFSVVHDPTQLDYQGPDSGPQYRSNIFYASGSQRKIAEAYIAQLGQ
                     AKVFGRDIVTRVDPLKAFYPAEEYHQDFLQLNPGHPYIVINDLPKIDSLRKVFPTYYR
                     EPPITVKSH"
     gene            1876..2391
                     /locus_tag="VNU03_12800"
     CDS             1876..2391
                     /locus_tag="VNU03_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007323268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HWP75392.1"
                     /translation="MDRLPEWVRGHEAVLEALWTTTRWQAQRRLMYDRVVDVPRLVAA
                     WPEDGPGHPLLPELRDALAAHYARPLPRVSLAAYRSGQDSVAFHGDRLGPARDDAIVA
                     IVALGTRRRFLLRPTGGGRSRALDLGAGDLLVMGGTCQSTWEHALPKVAHAGLRISVQ
                     LRSAPVVAARY"
     gene            complement(2401..>2590)
                     /locus_tag="VNU03_12805"
     CDS             complement(2401..>2590)
                     /locus_tag="VNU03_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75393.1"
                     /translation="HPELVAERIARYARLVGRENVIAGTDCGFGTWVGQAAVDPDIAW
                     AKLGSLAEGARLASRELW"
BASE COUNT          340 a          906 c          974 g          370 t
ORIGIN      
        1 agtgccggcg agggccaggg ctccgtcttc accgtcaagc ttcccgtcgc gatcgcccgc
       61 gccggcgcag tggtggacgc gtcgccgtcc gccgggcggg tgcacccgac cgcgccggcc
      121 ggggtgccgg cgcgagggcc atcgctgggc gggctgcggg tgctggtggt ggacgacgac
      181 cgggacgggc tcgacctggt cgcgacgatc ctgatgaaca gcggggccga ggtgcgggcc
      241 tgcgcgtcgg tggcggaagg gttcgaggcc ctccaggcat ggcgcccgga tgtcctcgtc
      301 tccgacatcg agatgccggg tgaggacggc tatgcgctca tccgtcgcgt ccgcgggctc
      361 gcgagcggtc ccgtcgcccg gacgccggcg gtggccctca ccgcctacgg gcgcgtcgag
      421 gatcggctac ggacgctcgc ggccggctac agcatgcacg ttccgaagcc ggtggatccc
      481 gccgagctgg ccaccatcgt ggcgagcctc gccggccggc cctaggcccg cgctcagacg
      541 cggcggggga gggtctggcc cttcaccggg ttggcgaccg cctcctcgag agtcttcgcc
      601 ggccgccact cgtcggccgg gcgagcggct cccaccgtgc cgacctggtc gatgttccag
      661 tagatctcga ggttgttgcc gtcggggtcc cggaactcga tggcgatctg gcagccggcg
      721 cggcggcggc cctcgaagtg gatcggcacg ccccgctcgc gcagccacgc gcgggcccgg
      781 aacacctcgt cgagcgagcc cacctcgaac gcgaagtgat tgaggctggt gtgctcgctg
      841 cccggcgcgc ccccgacgag ggcgaccccg tggtggtccg tgttcacccg gaggaagacc
      901 atcccgccgg gcaccatcga atcggggtaa cggtcgctga cctcgaggcc gagcacgtcc
      961 gtgtagaagg tcaccgcgcg ggtgaggtcg gtgacgttga gcacgacgtg gccaatcttc
     1021 cggagctgga actgcatcgc gcgcccggct cctagtggct cttcacggtg atgggcggct
     1081 cccggtagta ggtcgggaag accttccgga ggctgtcgat cttgggcaga tcgttgatca
     1141 cgatgtaggg gtgacccgga ttcagctgca ggaagtcctg gtggtactcc tcggccgggt
     1201 agaacgcttt cagaggatcg acccgcgtga cgatgtcgcg gccgaacacc ttggcctggc
     1261 cgagctgggc gatgtacgcc tccgcgatct tccgctgcga gccactcgcg tagaagatgt
     1321 tcgagcggta ctgggggccc gagtccggcc cctggtagtc gagctgggtg ggatcgtgta
     1381 ccaccgagaa gaagatctgg aggatctcgc cgtacgagat ctccttcggg tcaaagcgga
     1441 tctggaccga ctccgcgtgg cgggtccggc ccgcgctcac cgcctcgtag gtcgcggtcg
     1501 cccggtcgcc ccccgcgtag cccgagagga cctgccgcac gccgcgcacg tgctcgtaca
     1561 cgccctgcac gccccagaag cagccgcccg cgagcaccgc ggtctggacg ggccccgccg
     1621 ccttgacgtc gttcaccgcg ggggcgggaa tgacgaccgc cgattcggcg gcgggcgcgg
     1681 aggaaggcgc gccgacgaga gcaccgagga gcacggccac gagcagccag atcatgggga
     1741 gcctgatcgg atcctaccgc ttccggggcg tgagagcatg gagaagatgc tgtaccaggc
     1801 gggtctcttc ggcgaggcgc ccgcgtgcga cccgacgctg gcgggcgtgc ggcgcacgga
     1861 tctgggcgcg ggggcgtgga tcgactgccg gagtgggtcc ggggccacga ggcggtgctg
     1921 gaggcgctgt ggaccaccac ccgctggcag gcgcagcgcc gcctcatgta cgaccgcgtc
     1981 gtcgacgtgc cgcggctcgt ggccgcctgg ccggaggacg gccccggcca cccgctgttg
     2041 ccggagctgc gcgatgcgct ggccgcgcac tacgcgcgcc cgctgccgcg ggtgtccctc
     2101 gccgcctacc gcagcggcca ggacagcgtc gccttccacg gggatcggct ggggccggcg
     2161 cgcgacgatg cgatcgtggc gatcgtcgcg ctcggcacgc ggcgccgctt cctgctccgg
     2221 cccaccggcg ggggacgctc gcgcgcgctc gacctgggcg cgggcgatct cctggtgatg
     2281 ggcggcacct gccagagcac ctgggagcac gccctgccca aggtggccca cgcgggcctg
     2341 cggatcagcg tgcagctccg ctcggccccg gtggtggcgg cgcgctactg aggtccggac
     2401 ctaccagagc tcgcgtgagg cgagccgggc gccctcggcc aggctgccga gcttcgccca
     2461 cgcgatgtcg ggatcgaccg cggcctggcc cacccaggtg ccgaagccgc agtcggtccc
     2521 cgcgatgacg ttctcgcggc cgaccaggcg cgcgtagcgc gcgatccgct cggccaccag
     2581 ctcgggatgc
//
LOCUS       DATMCV010000557         2846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_763888, whole genome shotgun sequence.
ACCESSION   DATMCV010000557 DATMCV010000000
VERSION     DATMCV010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763888"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1187
                     /locus_tag="VNU03_12810"
     CDS             90..1187
                     /locus_tag="VNU03_12810"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75394.1"
                     /translation="MSAGALTPVQAARAAAGRERGRAPTLYLRLAVVLAWVLLPAAGP
                     SFQVLDLAVKIALFGALAASYDVVLGYTGIVSFGHAMFFGFGAYAVALAVGRLGAPTY
                     GHLLLGFLAGIAVSAVVAAAVGAFSLRVKALFFAMITLAFAEFALILAVQWGALTGGE
                     DGLSPKLPGVFAAGAPGALGLSGRLVTYYTLLAMCLGLFLLMSRFVHSPLGRTLQAIR
                     DNELRAEALGYRTFVFQLVATSFGSVVATVLGGGYVMWVRYVNPESALGIPIMLDVLL
                     MVIIGGIGTLHGGVVGAAILLTARTLLPNLRGLGGSLFAGSEILQRLSERWLLYFGIL
                     FVLVVFFFPRGVLGTLREAAARRRGPAAPGT"
     gene            1230..2288
                     /locus_tag="VNU03_12815"
     CDS             1230..2288
                     /locus_tag="VNU03_12815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP75395.1"
                     /translation="MPARDYDAIVVGASFGGLAVARQLRGEVLLLDRNEVGTVQTSAC
                     GTPLWVPETLGVSDSVLQVHDRLLIRTPTRTVRYDLSSVPFCTFDYRAFCQGLLAQCH
                     VRFLRTSVSGIQDGAVVTGEGRFTARVIVDCSGWRRAITGGAETEPRSRHHSFGLETH
                     TPLQDEGLTFLLDRRLIPQGLGWIFPVGSGSLIGLGSYVGRSKLKPALERLLRDHGMS
                     AGSYHGTYFPNRLLRPTVDQVFAVGDAAGQCLPLTAEGIRPALYFGGECGRIVQQVLD
                     GALPLEAGLERYRRLVARYRRPYRVLRFAQAMAAHTPTRWFAMVTQIAATWPFLSGWW
                     PRYGWFGRPDPAGVAAAT"
     gene            2349..>2846
                     /locus_tag="VNU03_12820"
     CDS             2349..>2846
                     /locus_tag="VNU03_12820"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWP75396.1"
                     /translation="MSTRTRWAIVSGVTGLIVAYCVWLVWTNAPAYQFLVRLYVDKLF
                     LKRTLREWGVLAPVIFIMLQALQVIISPIPGEATGILGGYLFGQWLGLFYSTIGLTLG
                     SVVAFWIGRWLGAHYVKNLVSAETWNRLGFIVEAEGAVLCFIVYLIPGLPKDIVCYLF
                     GISPMP"
BASE COUNT          313 a         1017 c          987 g          529 t
ORIGIN      
        1 ctggtgccca aggccgcgat cggcgtgacg atgggcatca tggtgctggt gctgctcgtg
       61 cgccccaccg ggctctacgg agccggcaag tgagcgcggg tgcgctcacc ccggtgcagg
      121 ccgcccgcgc ggcggcgggc cgcgagcgcg ggcgggcgcc caccctctac ctgcgcctgg
      181 cggtggtgct ggcgtgggtg ctgctcccgg ccgcgggccc gtccttccag gtgctcgacc
      241 tggcggtgaa gatcgcgctc ttcggcgcgc tcgccgcgag ctacgacgtg gtgctcggct
      301 acaccggcat cgtctccttc ggccacgcga tgttcttcgg cttcggagcc tacgcggtgg
      361 cgctcgcggt gggccgcctg ggcgcgccca cctacggcca tctcctgctc gggttcctgg
      421 ccggtatcgc ggtgtccgcg gtggtggcgg cggcggtggg ggccttctcg ctgcgggtca
      481 aggcgctctt cttcgcgatg atcaccctgg ccttcgccga gttcgcgctc atcctcgcgg
      541 tccagtgggg cgcgctcacc ggcggcgagg acggactctc cccgaagctg cccggcgtct
      601 tcgcggcggg cgcgccgggc gcgctcgggc tctccggccg cctcgtcacc tactacacgc
      661 tgctcgcgat gtgtctcggg ctcttcctgc tgatgagccg cttcgtgcac tccccgctcg
      721 gccgcacgct ccaggcgatc cgtgacaacg agctgcgcgc ggaggctctg ggctaccgca
      781 ccttcgtctt ccagctcgtg gccacgtcgt tcggcagcgt ggtcgcgacc gtgctcgggg
      841 gcggctacgt gatgtgggtg cgctacgtga atccggagtc ggcgctcggc atcccgatca
      901 tgctcgacgt gctcctgatg gtgatcatcg ggggcatcgg gacgctccac ggcggcgtcg
      961 tgggcgccgc gatcctcttg accgcccgca ccctgctacc gaacctgcgc gggctgggcg
     1021 gctcgctgtt cgcgggctcg gagatcctgc agcgcctgtc cgagcgctgg ctcctctact
     1081 tcggcattct cttcgtgctg gtggtgttct tcttcccgcg cggggtgctg ggcacgctgc
     1141 gcgaagcggc cgcccgacgg cgcgggccgg cggccccggg gacgtgaccc gttcatttgc
     1201 tcggcctttc gggtatgctg tgcctgaaaa tgcccgcccg ggattatgac gccatcgtgg
     1261 tgggggccag cttcggaggc ctcgctgtcg cccgccagct ccggggtgaa gtgctcctcc
     1321 tggatcggaa cgaggtcggc acggtgcaga cgtccgcctg cggaacccct ctctgggtgc
     1381 ccgagacgct cggggtctcc gacagtgtcc tccaggtcca cgaccggctc ctgatccgga
     1441 ccccaacccg caccgtccgg tacgatctct cctcggtgcc cttctgcacc ttcgactacc
     1501 gcgccttctg ccagggactg ctcgcccagt gccacgtgcg atttttgcgt acctcggtgt
     1561 cggggatcca ggacggggcg gtggtgaccg gggaaggccg cttcaccgcg cgggtgatcg
     1621 tcgactgctc gggctggcgc cgcgcgatca ccggcggcgc ggagacggag cctcgctcgc
     1681 gccaccactc cttcggcctc gagacccata cccccctgca ggacgagggg ctgaccttcc
     1741 tgctggaccg ccggctcatc ccccaggggc tcgggtggat ctttccggtg gggagcggga
     1801 gcctgatcgg tctggggtcg tacgtgggcc gctccaagct caagcccgcg ctcgagcgct
     1861 tactgcgtga tcacggaatg tcggcgggct cgtaccacgg cacctatttt cccaaccgcc
     1921 tgctccggcc caccgtcgac caggtcttcg cggtcggcga tgcggccggc cagtgcctgc
     1981 cgctcaccgc ggaaggcatc cggcccgcgc tctacttcgg cggcgagtgc ggccgcatcg
     2041 tgcagcaggt cctcgacggc gcgctgccgc tcgaggcggg gctcgagcgc taccgccggc
     2101 tggtcgcccg ctaccgccgc ccgtatcgcg tgctccggtt cgcgcaggcg atggccgccc
     2161 acactccgac tcgctggttc gccatggtca cccagatcgc cgcgacgtgg cccttcctgt
     2221 ccggctggtg gccccgatac ggatggttcg ggcgaccgga tcccgccggg gtagccgcgg
     2281 ccacctgagg cccatgtggt gaagagccgg gcgtcagggc cgatgccgcg tcacgggggc
     2341 cgggtggaat gagcacccgc acccggtggg cgatcgtgtc cggggtgacc gggctcatcg
     2401 tggcctactg cgtgtggctg gtctggacga acgcgcccgc ctaccagttc ctcgtccggc
     2461 tctacgtcga caagctcttt ctcaagcgca ccctgcggga gtggggcgtc ctcgcccccg
     2521 tcatcttcat catgctccag gccctgcagg tcatcatctc gcccatcccc ggcgaggcca
     2581 ccggcatcct cggcggctac ctcttcggcc agtggttggg gctgttctac tcgaccatcg
     2641 gtctcacgct gggctcggtg gtggccttct ggatcggacg gtggctcggg gcgcactatg
     2701 tgaagaatct ggtcagcgcc gagacgtgga accggctcgg gttcatcgtg gaggccgagg
     2761 gggcggtgct ctgcttcatc gtgtacctga tccccggcct gccgaaggac atcgtctgtt
     2821 acctcttcgg catcagcccg atgcct
//
LOCUS       DATMCV010000558         4276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_630086, whole genome shotgun sequence.
ACCESSION   DATMCV010000558 DATMCV010000000
VERSION     DATMCV010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630086"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="VNU03_12825"
     CDS             <1..1241
                     /locus_tag="VNU03_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP75397.1"
                     /translation="RAPDATLLLGKARLSLGQHEAALQAFRQAQTLSPVPGRPDEARF
                     WEGETLFRMKRFPEARAIYDRILTDNPNSPSAPDAVYGLAWVNLEQKRRDQAVTEFRR
                     LLSSYPENANVPSATFYLARTLTELKRPDEAIGLLRGFVSKYPDHRLLPDARYTLGQA
                     LIAGGQSDEGVGELRAFAKAYPSSELAPTARRTVVDTLVRQGKKSELAEEYKTLVAQS
                     PATAEGLYDAGLVAGKLGRPRDADTAWARLRKDFPDHPLSGRVSLEMAQSAFAKNSFK
                     DAATLAKAASRSPEDAVRGEAFVLLGESELKQKRHAAAVAAFKSAAEAPAVEPAMRYR
                     ALAGTGLAYEEQRQWAQAAKFYDEVASKSPDKTLASWAKERLAAVNTNAKSDKGDAKP
                     APKAPAKGSTGKSGGQGTKP"
     gene            1238..4228
                     /locus_tag="VNU03_12830"
     CDS             1238..4228
                     /locus_tag="VNU03_12830"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP75398.1"
                     /translation="MSRPRALFLAALLLLAPGSAARAEIALILPPPDPAALVPLASPP
                     LDKPPVPLPAVALPPAPVGMPELPPAPMVGNLGERPVAPLAPPRFLACNPVGSLFGVA
                     SELIECGRARYQRGELDEARDVLQRAVQEATERGQIREARYWLGETLLRQGKPAEVER
                     LMQQVQQDEPRGELGLYATHELGWVSLELGKPDRALTYFDTLLKTGAPPVLVPYARHG
                     RGLALYGLKRYPEARDTWVGLLNQSAPRAVAWEGTFWLGDSLGRLGDYPGAVQRLQTF
                     VSGGPQTLIDSGLLRLGWWSRAAGQPLDAAKAYRGMLSAYPRAREASWARAGLVQALL
                     DLDDYAAARDEAKKLEQADRNGPLVVPVRLLMSRWATDKARLDEAREINDDLLARTLD
                     PATRSYVLALSAETSRLNRQLDEARDRFELVRTAPGSPALAAYAALRLAQMDFDTREF
                     ARARDGARKLAADAAAPAEVRAAAQVLGGEAAYWARDYAQAAALYGGFLSSYPSQPQA
                     PMVSLALGWAELRGGKPDAARQRWARFAQQAQADPRAPEALVLAAELAGRAGDWAGAQ
                     SLLDQVVDRYPNSEYAEVATLNRAILAVRAGRAADTLPELGRLVNRGVVSPYVGRARA
                     TRGAALLATGKPAEAERDFQAALGQGETGLAQLGLGSASFARGQWDQAARQFADARDA
                     GAGPVADAAGYGLAAVLFNLRKIDEFKQVATPMVAGPVDPATTPRLVHALAAVAIEEK
                     RWSDARTLTLRLADQFPRYEGTQPALTQLAAAAGQGEQWALSRDMYAKLGERFPGSPA
                     SAGNRLDYAEALLRTGGAAEARRTLETGAATGAGQSPSVRASMLLAEAQEATGDRAAA
                     LETYTRVAAAGTGKEKAPGLLGQGRLLAADGKWDEARGRLEQALDAGDGAVAAEAAYR
                     LGEGYRSTGKAQDAADMYLTAAYLPSDSPWARRALVGAGQAFAALKQNDAAIIVYRKL
                     LAMNGVEADLAEAARRGLRALGAS"
BASE COUNT          568 a         1624 c         1600 g          484 t
ORIGIN      
        1 cccgcgcgcc cgatgcgacg ctgctcctcg gcaaggcgcg cctgtccctg ggccagcacg
       61 aggccgcgct ccaggccttc cgccaggccc agaccctctc gccggtcccc ggccggccgg
      121 acgaggcgcg cttctgggaa ggtgagacgc tgttccggat gaagcgcttt ccggaagcgc
      181 gggccatcta cgaccggatc ctcaccgaca atccaaactc gcccagcgcg cccgacgcgg
      241 tgtacggcct cgcctgggtg aatctcgagc agaagcgccg cgaccaggcg gtcaccgagt
      301 tccgccgcct gctctcgagc taccccgaga acgccaacgt tccctcggcc accttctacc
      361 tggcccgtac gctcaccgag ctgaagcgcc ccgacgaggc gatcggcctg ctgcggggct
      421 tcgtctccaa gtatcccgac caccgactgc tgccggacgc ccgctacacg ctgggccagg
      481 ccctgatcgc gggggggcag agcgacgagg gcgtgggcga gctgcgcgcc ttcgccaagg
      541 cctacccgag ctccgagctg gcccccaccg cgcggcgcac cgtggtcgac acgctcgtgc
      601 ggcagggcaa gaagagcgag ctggccgagg agtacaagac gctggtcgcg cagtcccccg
      661 ccaccgcgga agggctctac gacgcgggcc tcgtggcggg aaagctcggc cggccgcgcg
      721 acgcggacac cgcgtgggcg cgcctgcgga aggatttccc cgaccacccg ctgagcgggc
      781 gcgtctcgct cgaaatggcc cagagcgcct ttgccaagaa tagcttcaag gacgcggcca
      841 ccctcgcgaa ggcggcgtcc cgcagcccgg aggacgcggt gcgcggcgag gcgttcgtgc
      901 tgctggggga gagcgagctc aagcagaagc gccacgccgc cgccgtcgcg gccttcaagt
      961 ccgccgccga ggcccccgcg gtggagccgg ccatgcgcta ccgggccctg gccggcaccg
     1021 gcctcgccta cgaggagcag cgccagtggg cgcaggccgc gaagttctac gacgaggtgg
     1081 cgagcaagag cccggacaag acgctcgcct cctgggccaa ggagcggctg gccgcggtca
     1141 acaccaacgc caagtccgac aagggcgacg ccaagccggc gccgaaggcg cccgccaagg
     1201 gatcgaccgg caagtcgggt gggcagggga ccaagccatg agccggccgc gcgcgctatt
     1261 cctcgccgcg ctcctgctgc tcgcgccggg ctcggccgcg cgggccgaga tcgcgctgat
     1321 cctgccgccg ccggacccgg ccgccctcgt cccgctcgcc tcgccgccgc tcgacaagcc
     1381 gccggtgccg ctgcccgcgg tcgcgctgcc gccggcgccg gtgggcatgc ccgagctgcc
     1441 gccggccccg atggtgggca acctgggcga gcgaccggtg gccccgctgg cgccgccgcg
     1501 cttcctcgcg tgcaacccgg tgggcagtct cttcggggtg gcctccgagc tgatcgagtg
     1561 cgggcgcgcg cgctaccagc gcggcgagct cgacgaggcg cgcgacgtgc tccagcgcgc
     1621 ggtgcaggag gcgaccgagc gcgggcagat ccgcgaggcg cggtactggc tcggcgagac
     1681 cctgctgcgc cagggcaagc ccgccgaggt cgagcggctc atgcagcagg tgcagcagga
     1741 cgagccgcgc ggcgagctcg gcctctacgc gacccacgag ctcggctggg tatcgctcga
     1801 gctgggcaag cccgatcggg cgctcaccta cttcgacacc ctgctcaaga ccggcgcgcc
     1861 gccggtgctc gttccctacg cgcgccacgg gcgcgggctc gccctctacg ggctcaagcg
     1921 ctacccggag gcgcgcgaca cctgggtcgg gctcctcaac cagagcgcgc cccgtgcggt
     1981 ggcgtgggaa gggacgttct ggctcggcga cagcctgggc cgcctcggag actacccggg
     2041 cgcggtgcag cggctgcaga ccttcgtctc cggcggtccg cagaccctca tcgacagcgg
     2101 gctcttgcgc ctcggctggt ggagccgcgc cgcgggccag ccgctcgacg cggccaaggc
     2161 ctaccggggc atgctctcgg cgtacccccg ggcgcgcgag gcatcctggg cgcgcgccgg
     2221 cctcgtccag gcgctgctgg acctcgacga ctacgcggcc gcgcgcgacg aggccaagaa
     2281 gctcgagcag gcggaccgga acggtccgct cgtggtgccg gtgcggctcc tgatgagccg
     2341 ctgggccacc gacaaggcgc ggctggacga ggcccgcgag atcaacgacg acctgctggc
     2401 gcgcaccctc gaccccgcga cccgctccta cgtgctggcg ctcagcgcgg agacgagccg
     2461 gctcaaccgt cagctcgacg aggcgcgcga ccgcttcgag ctggtgcgca ccgcccccgg
     2521 ctcgccggcc ctggccgcgt acgccgcgct ccgtctggcc cagatggact tcgatacgcg
     2581 cgagttcgcg cgggcccgcg acggcgcccg caagctcgcg gccgacgccg cggccccagc
     2641 cgaggtgcgc gcggccgcgc aggtgctggg cggcgaggcg gcctactggg cgcgcgacta
     2701 cgcgcaggcg gcggcgctct acggcggctt cctgtcgagc tatccgagcc agccgcaggc
     2761 gccgatggtg agcctggcgc tcggctgggc cgagctacgc ggcggcaagc ccgacgcggc
     2821 ccgccagcgc tgggctcggt tcgcccagca ggcgcaggcc gatccgcgcg cgccggaagc
     2881 gctcgtcctg gccgcggagc tggccgggcg cgccggggac tgggccgggg cccagagcct
     2941 gctcgaccag gtggtggacc gttaccccaa cagcgagtac gccgaggtcg cgacgctcaa
     3001 ccgcgcgatc ctcgcggtgc gcgctggtcg cgcggccgac accctcccgg agctgggccg
     3061 cctcgtgaac cgcggcgtgg tctcgccgta cgtgggccga gcccgggcca cgcgcggcgc
     3121 ggcgctgctc gccaccggca agccggccga ggccgagcgc gacttccagg ccgccctcgg
     3181 ccagggtgag accgggctcg cccagctggg cctcggcagc gcgagcttcg cgcgcgggca
     3241 gtgggatcag gcggcgcgcc agttcgcgga cgcgcgggac gcgggcgcgg gcccggtggc
     3301 ggacgcggct ggctacggcc tcgccgcggt gctcttcaat ctgcgaaaga tcgacgagtt
     3361 caagcaggtg gcgacgccga tggtggcggg gccggtcgat ccggccacca cgccgcggct
     3421 ggtgcacgcc ctcgccgcgg tcgcgatcga ggagaagcgg tggagcgacg cgcggacgtt
     3481 gaccctccgc ctggccgacc agttcccgcg ctacgagggc acgcagcccg cgctcaccca
     3541 gctcgcggcc gccgccggcc agggcgagca gtgggcgctc tcgcgggaca tgtacgccaa
     3601 actcggtgag cgcttcccgg gcagcccggc cagcgcgggc aaccggctcg actacgcgga
     3661 ggcgctgctg cgcaccggcg gggccgccga ggcccgccgc acgctcgaga ccggcgccgc
     3721 gacgggtgcg ggccagtcgc cctccgtccg ggcctcgatg ctgctcgccg aggcgcagga
     3781 ggccaccggc gatcgggccg ccgcgctcga gacctacacg cgtgtggccg ctgcgggcac
     3841 cgggaaggag aaggccccgg ggctgctcgg gcaggggcgg ctcctcgccg ccgacggcaa
     3901 gtgggacgag gcgcgcggcc ggctcgagca ggcgctcgac gcgggcgacg gggcggtggc
     3961 cgcggaggcg gcgtatcgcc tcggcgaggg ctatcgctcc accggcaagg cgcaggacgc
     4021 ggccgacatg tacctgaccg ccgcctatct cccgagcgat tcaccctggg cgcggcgggc
     4081 gctcgtcggc gccggccagg ccttcgccgc gctgaagcag aacgacgccg cgatcatcgt
     4141 gtaccgcaag ctgctcgcga tgaacggcgt cgaggccgac ctggccgaag ccgcgcgccg
     4201 cggccttcgc gccctcggcg cctcctagcc tctcccgctc ccctcagggg agagggcagg
     4261 gtgaggggcg ggccgg
//
LOCUS       DATMCV010000559         3246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_27839, whole genome shotgun sequence.
ACCESSION   DATMCV010000559 DATMCV010000000
VERSION     DATMCV010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27839"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VNU03_12835"
     CDS             complement(<1..579)
                     /locus_tag="VNU03_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75399.1"
                     /translation="MFSTPGSDIWPSPLRPIAALGRLSLEMVQGLGQFGRFFLQAAAY
                     TVIPPFKFGRLVDRIHFIGFRSLTIVVLTGAFTGMVLGLQIFLTLSRFGSEAYLGPAV
                     ALSLIRELGPVLCAVFVTGLAGSALTAEIGIMKITEQIDALQVMALNPTRYLVVPAIV
                     AGVITFPLLVSIFDVVGILGGYLVGVELLGLSP"
     gene            658..1488
                     /locus_tag="VNU03_12840"
     CDS             658..1488
                     /locus_tag="VNU03_12840"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HWP75400.1"
                     /translation="MAIIDGALTGFASVAGQLVGTFQLRPYVFGFLAFFLVAGLRDLG
                     RARTVTFLLWGGAVALVAELVSTRVGIPFGLYHYTGDTRAEELFLGDVPFFSPLSFPF
                     LAYAAFCLARRTLGPGWVATRAGRVRLVAVSGALMMLLDVVIDPLAVRGGRWFLGHIF
                     YYPDGGLYFGVPLSNFLGWLVVGWITIGGIVWAEALAGREATDPGAPGLGIALYYLVL
                     AVNLSVTLWIGEELIAAAGIVVHGGLFLLLYGVSRVTVGRWSAESLSAPAMTGGETRP
                     "
     gene            1511..2527
                     /gene="hpnH"
                     /locus_tag="VNU03_12845"
     CDS             1511..2527
                     /gene="hpnH"
                     /locus_tag="VNU03_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HWP75401.1"
                     /translation="MSVPLSQMWTVATYVLRQKLARRKRYPFVLMLEPLFRCNLACAG
                     CGKIQYPAQILKKNLSVEKCLAAVEECGAPIVSIPGGEPLMYPDIDRLVAELVKRKKY
                     VYLCTNAILLKEKLDLFTPSKYLSFSVHMDGLREEHDEAVCRDGVYDQAVPAIREAVA
                     RGFRVTTNSTLFDGASPLRMREFFDEMMSLGVEGMMVSPGYSYSKAPDQEHFLLQSRT
                     HELFSKMLASPKKRWRFNQSPLFLRFLMGKHDFECTPWGNPTYNMFGWQRPCYLLQEG
                     YARTFQELMDTTLWENYGKKSGNEKCRDCMVHCGYEATAVDYTFSSLKGFVETVGVTL
                     TGKL"
     gene            2538..2894
                     /locus_tag="VNU03_12850"
     CDS             2538..2894
                     /locus_tag="VNU03_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75402.1"
                     /translation="MSLEGWVPDVGQYLTLAEVVEFAFDYRGNTTIVKTDGTEFVGYI
                     FNRNTYGPEPFIEYFDESGEGPFTLRYSELANVKFTGKDTAAGNSWKAWLERKEREKA
                     ERVPAQVVPDPDPHRG"
     gene            2863..>3246
                     /locus_tag="VNU03_12855"
     CDS             2863..>3246
                     /locus_tag="VNU03_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75403.1"
                     /translation="MSRILILTAVELEARALARQLELPALPSLPFPAFGRGTLRLAPV
                     GLRAALLAERWPGLRLGLDQPLVISAGVCGALAPDLRSGDLILPESVIGPSAERLNVT
                     PTAYARAAGLGPAARHGTLVTTRDVV"
BASE COUNT          508 a         1099 c         1119 g          520 t
ORIGIN      
        1 gggggagagc ccgagcagct cgacgccgac caggtagccg cccaggatgc cgaccacgtc
       61 gaagatcgag accaggagcg ggaacgtgat gacgcccgcc acgatggccg gcaccaccag
      121 gtaacgcgtc gggttcagcg ccatgacctg cagcgcgtcg atctgctcgg tgatcttcat
      181 gatgccgatc tcggcggtca gggccgagcc ggcgaggccg gtcacgaaga ccgcgcagag
      241 caccgggccc agctcgcgga tgagcgacag cgccaccgcc gggcccaggt aggcctccga
      301 cccgaagcgc gagagggtca ggaagatctg caggccgagg accatgccgg tgaaggcgcc
      361 ggtgagcacc acgatggtca gcgaccggaa gccgatgaag tggatccggt cgaccaggcg
      421 gccgaacttg aagggcggga tgacggtgta cgcggcggcc tggaggaaga accggccgaa
      481 ttgcccgagc ccctggacca tctcgaggct cagacgcccc agcgcggcga tgggccgcag
      541 cggcgacggc caaatatcgg atcctggggt cgaaaacatc gctagatact agcatagggg
      601 tcggcgaatc ccggcgccgt tcttgaaaaa aacggccgac ttccgtaagg tgaacgtgtg
      661 gcgatcatcg acggcgcact caccggcttc gcgtcggtcg cgggccagct ggtcgggacc
      721 ttccagcttc gcccgtacgt gttcggcttc ctggccttct tcctggtggc ggggctgcgg
      781 gacctcgggc gtgcccggac ggtcacgttc ctgctctggg gcggcgcggt ggccctggtc
      841 gccgagctgg tctccacgcg ggtggggatc ccgttcggcc tctaccacta cacgggcgac
      901 acccgggccg aggagctctt cctcggcgac gtgccgttct tctcgccgct gtcgtttcct
      961 ttcctcgcct acgccgcgtt ctgcctggcg cgccggaccc tcggcccggg gtgggtcgcg
     1021 acccgcgccg gtcgggtccg cctggtggcg gtgtcggggg cgctgatgat gctcctcgac
     1081 gtcgtcatcg atccgctggc ggtgcggggc ggccgctggt tcctcggcca catcttctac
     1141 tacccggacg ggggcctcta cttcggggtg ccgctctcca acttcctggg ctggctggtc
     1201 gtcggctgga tcacgatcgg cgggatcgtc tgggcggagg cgctggccgg tcgcgaggcg
     1261 accgacccgg gcgcgcccgg gctcgggatc gccctgtact acctcgtcct cgcggtcaac
     1321 ctgagcgtga cgctctggat cggcgaggag ctgatcgcgg ccgcgggaat agtcgtccac
     1381 ggcgggctct ttctgctact gtatggcgtc agtagagtga cggtcggccg atggtcagcc
     1441 gagtccctgt ccgcccccgc catgaccggc ggggaaacga ggccctgagg ggccgaaagg
     1501 gtcgtcgaac atgagcgttc cgctgtctca gatgtggacg gtcgcgacct acgtgctgcg
     1561 ccagaagctg gcgcggcgca agcgctatcc gttcgttctg atgctcgagc cgctcttccg
     1621 ctgcaacctc gcctgcgccg gctgcggcaa gatccagtac ccggcccaga tcctcaagaa
     1681 gaacctgagc gtggagaagt gcctggccgc ggtcgaggag tgcggcgcgc cgatcgtgag
     1741 catcccgggc ggcgagcccc tgatgtaccc ggacatcgat cgcctggtcg ccgagctggt
     1801 gaagcggaaa aagtacgtgt atctctgcac caacgccatc ctcctgaagg agaagctcga
     1861 tctcttcacc ccgtcgaagt acctctcgtt cagcgtccac atggacggcc tgcgcgagga
     1921 gcacgacgag gcggtgtgcc gcgacggcgt ctacgaccag gcggtgcccg cgatccggga
     1981 ggcggtggcg cgcggcttcc gggtcaccac caacagcacg ctcttcgacg gggcgtcccc
     2041 gctccggatg cgcgagttct tcgacgagat gatgagcctc ggggtggaag gcatgatggt
     2101 ctccccgggc tacagctact cgaaggcgcc cgaccaggag cacttcctcc tgcagtcgcg
     2161 cacccacgag ctgttctcca agatgctggc gagcccgaag aagcgctggc gcttcaacca
     2221 gtcgcccctc ttcctgcgct tcctgatggg caagcacgac ttcgagtgca ccccgtgggg
     2281 caacccgacc tacaacatgt tcgggtggca gcggccgtgc tacctcctgc aggaagggta
     2341 cgcgcgcacc ttccaggagc tgatggacac cacgctctgg gagaactacg gcaagaagag
     2401 cggcaacgag aagtgccgtg actgcatggt gcactgcggg tacgaggcca ccgcggtgga
     2461 ctacacgttc agctcgctca agggcttcgt cgagacggtc ggggtcactc tgaccggcaa
     2521 gctgtaggga gcccgccatg agcctcgagg gctgggtgcc cgacgtcggc cagtacctca
     2581 cgctggcgga ggtggtcgag ttcgccttcg actaccgggg caacaccacc atcgtgaaga
     2641 ccgacggaac cgagttcgtc ggctacatct tcaaccgcaa cacctacggg ccggagccct
     2701 tcatcgagta cttcgacgag agcggcgagg gccccttcac gctccgctac tccgagctcg
     2761 ccaacgtcaa gttcaccggc aaggacaccg cggccgggaa cagctggaag gcctggctcg
     2821 agcgcaagga gcgggagaag gcggagcggg tcccggcgca ggttgtcccg gatcctgatc
     2881 ctcaccgcgg ttgagctcga ggcccgggcc ctcgcccgac agctcgagct tcccgcgctg
     2941 ccctcgctgc cctttcccgc cttcggtcgt ggcacgctgc ggctcgcccc ggtcggcctg
     3001 cgggcggccc tcctcgcgga gcgctggccg ggcttgcgcc tgggactcga tcagcccctg
     3061 gtgatctcgg ccggggtctg cggggcgctc gccccggacc tccggagcgg cgacctgatt
     3121 ctgcccgaga gcgtgatcgg cccctccgcg gagcgcctca acgtgacccc gaccgcctac
     3181 gctcgcgcgg ccgggctcgg gcccgccgcc cgccacggca cgctggtgac cactcgtgac
     3241 gtggtg
//
LOCUS       DATMCV010000560         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_562069, whole genome shotgun sequence.
ACCESSION   DATMCV010000560 DATMCV010000000
VERSION     DATMCV010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562069"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VNU03_12860"
     CDS             complement(<1..939)
                     /locus_tag="VNU03_12860"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75404.1"
                     /translation="MSTTGQTEARKLHASLSHPIIDADGHWVEYLPHMRQEFRRIGGD
                     TAVEALDFASRRIPASLAMSVAERNRRRIGQEAFWFLPTKNTLDRATAMLPRLMYERL
                     DDLGLDFTVIYPTNGLGYHRVPDARLRRALCRAYNVFTAEQFRPFGDRIIPAAIIPVH
                     TPEEAIEELEFASRQLGLRVVMMGGLVRRPVPTLAEEHPEASKFVEWYDVLGIDSPYD
                     YDPVWEKCRELRIAPSFHNGARTILLRNSPSNFCYNHIGHFASAGHAICKALFFGGVT
                     RRFPDLNFAFLEGGVGWASMLLADLIGHWEKRNPEGL"
     gene            complement(1348..1557)
                     /locus_tag="VNU03_12865"
     CDS             complement(1348..1557)
                     /locus_tag="VNU03_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75405.1"
                     /translation="MRESGLVGVGVVLGWLASKTWSDPGCQLGLVVFLVATAVMTLGF
                     LLAVVGHGRDARDGDHDDDDEGDRP"
     gene            complement(1567..1770)
                     /locus_tag="VNU03_12870"
     CDS             complement(1567..1770)
                     /locus_tag="VNU03_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75406.1"
                     /translation="MTMRSAASLALLGAILVAILLVVGFVLDLLNAVRGLIPALKLVT
                     SFIYAFASVGAVVFLCVFRKAQS"
     gene            1877..2695
                     /locus_tag="VNU03_12875"
     CDS             1877..2695
                     /locus_tag="VNU03_12875"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP75407.1"
                     /translation="MSDPDFSLDDVARYWDDNAAAWAREVGQGHDIAREFQNNPAFLA
                     LVGDLRGRQVLDAGCGDGYNTRMLARSGAHMTGVDLSARMIELAQDEERRAPLGIRYA
                     RTSYTDLAQFAGATFDAVVSFMALMDGPRFDLAMAECFRVLRPGGRLAFSITHPCFLT
                     RGSRWIRDEQGVKVSRLVGHYFDPARWVERFRFTDAPADAPEFVVPRFDRTLSEYVNT
                     LTGVGFVLKRLEEPRPSEEYCQAHPGQRGWREHVALFLHFLAEKPAEKPAEKPA"
BASE COUNT          431 a          982 c          959 g          399 t
ORIGIN      
        1 gagcccctcg gggttgcgct tttcccagtg cccgatgagg tcggccagga gcatggaggc
       61 ccagcccacc ccgccctcga ggaaggcgaa gttcaggtcc gggaagcgcc gggtgacgcc
      121 gccgaagaag agcgccttgc agatcgcgtg accggcggag gcgaagtgcc cgatgtggtt
      181 gtagcagaag ttcgacggcg agttccgcag caggatcgtg cgcgcgccgt tgtggaagct
      241 cggcgcgatg cgcagctcgc ggcacttctc ccacaccggg tcgtagtcgt aggggctgtc
      301 gatgccgagc acgtcgtacc actcgacgaa cttcgaggcc tcggggtgct cctcggccag
      361 cgtgggcacg ggacggcgga cgaggccacc catcatgacc acccggaggc cgagctgtcg
      421 cgaggcgaac tcgagctcct cgatcgcctc ctccggcgtg tgcaccggga tgatcgcggc
      481 cgggatgatc cggtcgccga acggccggaa ctgctcggcg gtgaacacat tgtaggcgcg
      541 gcagagcgcg cggcggagcc gggcgtcggg cacgcggtgg tagccgagcc cgttggtggg
      601 atagatgacg gtgaagtcga ggcccagatc gtcgagccgc tcgtacatga ggcgcggcag
      661 catggcggtc gcccggtcga gcgtgttctt ggtcggcagg aaccagaacg cctcctggcc
      721 gatccggcgc cggttgcgct ccgccaccga catcgcgagc gaggcgggga tgcgccggct
      781 ggcaaagtcc agcgcctcga cggcggtgtc gccgccgatg cgccggaact cctggcgcat
      841 gtgaggcagg tactcgaccc agtgcccgtc ggcatcgatg atcgggtggg acaggctggc
      901 gtggagcttc cgggcctcgg tctgaccggt cgtggacatg gacggcctcc tgggagcgtc
      961 agtctggcgc ggatcatacc cccgatccgc cctcttgcgc atgaggggag gacgtcgcct
     1021 gctacgggga cgacgacgcg gccgcgtgag cccgagaggt cggccagggt gacgacgtcg
     1081 cccgcgagtt cctgaacaat ccggccttcc tggctctcat cggcgatctg cgtggccgcc
     1141 aggtcctcga tgccggatgc ggcgacggct acaacacccg gatcctcgcg cgggccggcg
     1201 cgcacatgag cggcgggcta cccggagagt ggcagaccgc ccgcgccggc tcccacctgt
     1261 gcgtccgcat ctcgaagccg ctcgtggcga cgcgcgggcg ggtagacctc tccgaggtag
     1321 tgccggcgcg ggccgcgagg aaacatgtca gggccgatcg ccctcgtcat catcatcgtg
     1381 gtcgccgtcg cgcgcatccc gtccgtggcc gaccaccgcg agcaggaagc ccagggtcat
     1441 gaccgcggtg gcgaccagga agaccaccag ccccagctgg cacccgggat cgctccaggt
     1501 cttgctcgcc agccagccga ggaccacacc cacgccgacg agcccgctct ccctcaccgc
     1561 cctgcctcac gactgggctt tgcggaagac gcacaggaac acgaccgccc ccacgctcgc
     1621 aaacgcgtag atgaaggacg tgaccagctt cagcgccggg atgagaccgc gcaccgcgtt
     1681 cagcaggtcg aggacgaacc cgacgaccag caggatcgct accaggatag ctccgagcag
     1741 ggccagagac gccgcactcc gcatcgtcat gcgcccgagc ctatgctcaa ggggccggca
     1801 ggtcaacccg gccgccgcgc ccgcgccttc ggtttgacac gcgccgcccc atctggcagg
     1861 atcgccgctt ccggccatga gcgatcccga tttctccctc gacgacgtcg cccgctactg
     1921 ggacgacaac gccgccgcgt gggcccgcga ggtcgggcag ggccacgaca tcgcccgcga
     1981 gttccagaac aatccggcct tcctggcgct cgtcggcgat ctccgcggcc gccaggtcct
     2041 cgacgccgga tgcggcgacg gctacaacac ccggatgctc gcgcggagcg gcgcccacat
     2101 gaccggcgtg gatctctccg cccgtatgat cgagctggcc caggacgagg agcgccgcgc
     2161 cccgctgggg attcgctacg cgcgcacctc ttatacggac ctcgcccagt tcgccggggc
     2221 caccttcgac gcggtggtgt ccttcatggc cctgatggac gggccgcgct tcgacctggc
     2281 catggcggag tgcttccgcg tcctccgccc gggcgggcgg ctcgccttca gcatcaccca
     2341 tccctgcttc ctcacccgcg gctcgcggtg gattcgcgac gagcagggcg tcaaggtcag
     2401 ccggctggtg ggccactact tcgatccggc ccgctgggtg gagcgcttcc gcttcacgga
     2461 cgcgccggcc gatgcgcccg agttcgtcgt gccccgcttc gaccgcacac tctccgagta
     2521 cgtcaacacg ctcaccgggg tgggcttcgt cctgaagcgc ctcgaggagc cgcgtccgtc
     2581 ggaggagtac tgccaggcgc atcccggcca gcgtgggtgg cgcgagcacg tcgcgctctt
     2641 cctgcacttc ctggcagaga agccggccga gaagccggcc gagaagccag cctgaggctg
     2701 ctgcgggggc cgacggctag cgattcctcg cggccagggc cagcatcacc cacgaccagc
     2761 cgatgaagat c
//
LOCUS       DATMCV010000561         3310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_497150, whole genome shotgun sequence.
ACCESSION   DATMCV010000561 DATMCV010000000
VERSION     DATMCV010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497150"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VNU03_12880"
     CDS             complement(<1..189)
                     /locus_tag="VNU03_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75408.1"
                     /translation="MDRARVRALAERVLRLEGESVLALCARLDDRFVDAVAALHGCRG
                     RVIVTGMGKSGLIGRKIAA"
     gene            complement(189..1037)
                     /gene="kdsA"
                     /locus_tag="VNU03_12885"
     CDS             complement(189..1037)
                     /gene="kdsA"
                     /locus_tag="VNU03_12885"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HWP75409.1"
                     /translation="MTATRPVRIGAVTIGGGAPLALIGGPCAIENEKHALMMAERLQR
                     VTAAAGVPFIYKSSYDKANRSSIHAYRGPGLVEGLRILRKVKDETGLGLLSDVHDVSE
                     VAPAAAVLDVLQVPAFLCRQTDLIVACARSGRPVNVKKGQFVAPRDMINVVEKVRASG
                     SEDLLLTERGSSFGYNNLVVDFRGLPIMRSFGYPVVFDATHSVQLPGGQGDRSGGERQ
                     YVQALARAAVAVGVDALFMEIHEDPDRTLDDGRPLSDGPNMLRLDDLPRLLDEIRAIA
                     AGPGRP"
     gene            complement(1034..2644)
                     /locus_tag="VNU03_12890"
     CDS             complement(1034..2644)
                     /locus_tag="VNU03_12890"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWP75410.1"
                     /translation="MTTRFIFVTGGVVSSLGKGLAAASIGSLLEARGFRVTLQKMDPY
                     INVDAGTMSPYQHGEVYVTDDGGETDLDLGHYERFTSARMTRDNNITTGKVYGTVIEK
                     ERRGDYLGRTVQVIPHITDQIKASVRKVAAGVDVVIIEIGGTVGDIEGLPFLEAVRQF
                     KKDVGKDNVIYVHLTLVPYIQAAQELKTKATQHSVKELRAIGIQPDVLLCRTAKDHPL
                     TPSTKSKIALFCDVEEEAVIAARDVDTIYEVPLAFHDQGLDESIVKLLGLPARPIDLS
                     RWEEIVRRVKAPRRRCRIGVVGKYIEVKDSYKSLYEALAHGGIAHEAAVEVVWVDAER
                     IEREGLAGHVAGLDGILVPGGFGDRGIEGKIQAIRYAREQRVPYFGICLGMQCAVIEY
                     ARHVCGLEGANSTEFNPTPAHNVIDLLPEQRHIQDKGGTMRLGLYPVLLSEGTMASRV
                     YGQGLIEERHRHRYEVNNDYLARLEKDGLRISGIWAEKQLVEIIELPDHPWFVAGQFH
                     PEFRSRPWEPHPLFAAFIKAALDRQGSA"
     gene            complement(2646..>3310)
                     /locus_tag="VNU03_12895"
     CDS             complement(2646..>3310)
                     /locus_tag="VNU03_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75411.1"
                     /translation="RRSYVALSGQRLWREIELAAAEPCARQAFEHLVRSRVATLLGIS
                     NVSTGRLADADRLARWARAAGVAVDPAELFMLALLAGSSATAVARCLDRLRLSGEPRA
                     NLEAGAMAGPLARRLGAPRLRPSAVDDVLRPVPPAAALSAWLRGSAAARRRIEWYLAS
                     GRAVQPRLSGGDLLALGMPRGPQVGRALARLRRCRLDGAVGSVAEERELVKEWLTSGK
                     EA"
BASE COUNT          473 a         1224 c         1125 g          488 t
ORIGIN      
        1 cgccgcgatc ttgcgcccga tgaggcccga cttgcccatt ccggtgacga tgacgcggcc
       61 ccggcagccg tggagcgcgg ccaccgcgtc cacgaagcgg tcgtcgagcc gcgcgcagag
      121 ggcgagcacg ctctccccct cgaggcgcag cacccgctcc gcgagcgccc ggacccgcgc
      181 gcgatccatc aggggcgccc cgggccggcc gcgatcgcgc gaatctcgtc gagcaggcgc
      241 ggcaggtcgt ccaggcgcag catgttgggc ccgtccgaca gcgggcggcc gtcgtcgagc
      301 gtgcggtccg gatcctcgtg gatctccatg aacagggcgt ccaccccgac cgccaccgcg
      361 gcgcgcgcga gggcctgcac gtactgccgc tcgccgcccg agcgatcccc ctgcccgccc
      421 ggcagctgca ccgagtgggt ggcgtcgaac accaccggat agccaaagct ccgcatgatc
      481 ggcaggccgc ggaagtcgac caccaaattg ttgtaaccaa aactggagcc gcgctcggtc
      541 aacaggagat cctcgctgcc ggacgcgcgc accttctcca ccacgttgat catgtcgcgc
      601 ggggccacga actgcccctt cttcacgttg accgggcggc cgctccgcgc gcaggccacg
      661 atgaggtcgg tctgccggca gaggaacgcg ggcacctgca gcacgtcgag caccgcggcg
      721 gcgggcgcca cctccgagac gtcgtggacg tcggagagca ggccgagccc cgtctcgtcc
      781 ttgaccttcc ggaggatgcg gagcccctcc accagccccg gcccccggta ggcgtggatc
      841 gaggagcggt tggccttgtc gtaggacgac ttgtagatga acggcacgcc cgccgccgcg
      901 gtcacccgct gcaggcgctc cgccatcatc agcgcgtgct tctcgttctc gatcgcgcac
      961 gggcccccga tcagggccag gggcgcgccg cccccgatgg tgaccgcgcc gatccggacc
     1021 ggccgcgtcg cggtcacgcg ctcccctggc gatcgagcgc ggccttgatg aacgcggcga
     1081 agagcgggtg cggctcccac gggcgcgagc ggaactcggg atggaactgc ccggccacga
     1141 accacgggtg atcgggcagc tcgatgatct ccaccagctg tttctcggcc cagatgccgg
     1201 agatgcggag gccgtccttc tcgagccggg ccaggtagtc gttgttgacc tcgtagcggt
     1261 gccggtggcg ctcctcgatg agcccctgcc cgtagacccg cgaggccatc gtgccctcgc
     1321 tcagcagcac cgggtagagc ccgagccgca tggtgccgcc cttgtcctgg atgtgacgct
     1381 gctcgggcag gaggtcgatc acgttgtggg cgggggtcgg gttgaactcg gtggagttgg
     1441 cgccctcgag gccgcacacg tgccgcgcgt actcgatgac cgcgcactgc atgccgaggc
     1501 agatgccgaa gtagggcacg cgctgctcgc gcgcgtagcg gatggcctgg atcttcccct
     1561 cgatgccgcg gtccccgaag ccccccggca ccaggatccc gtcgagcccg gccacgtggc
     1621 ccgcgagccc ctcgcgctcg atgcgctcgg cgtccaccca caccacctcg accgcggcct
     1681 cgtgggcgat gcccccgtgg gcgagcgcct cgtagaggct cttgtaggag tccttcacct
     1741 cgatgtactt gcccaccacc ccgatgcgac agcggcggcg cggcgccttc acccggcgca
     1801 cgatctcctc ccagcgggag agatcgatcg gccgggcggg aagcccgagc agcttgacga
     1861 tggactcgtc caggccctgg tcgtggaagg cgagcggcac ttcatagatc gtgtccacgt
     1921 cgcgggccgc gatcaccgcc tcttcctcga cgtcacagaa gagcgcgatc ttggacttgg
     1981 tggagggcgt cagcggatgg tccttggcgg tccggcacag gagcacgtcg ggctggatac
     2041 cgatggcccg cagctccttg accgagtgct gggtggcctt ggtcttgagc tcctgcgcgg
     2101 cctggatgta cggcaccagc gtgaggtgga cgtagatgac gttgtccttg cccacgtcct
     2161 tcttgaactg gcgcaccgcc tcgaggaagg gcagcccctc gatgtcgccg acggtgcctc
     2221 cgatctcgat gatcaccacg tccaccccgg cggccacctt ccggaccgac gccttgatct
     2281 ggtcggtgat gtgcgggatg acttggacgg tgcggccgag atagtccccg cggcgctcct
     2341 tctcgatcac ggtcccgtag accttcccgg tggtgatgtt gttgtcccgg gtcatgcgcg
     2401 ccgaggtgaa gcgctcgtag tggccgaggt cgaggtcggt ctcgccgccg tcgtcggtga
     2461 cgtagacctc gccgtgctga tacggactca tggtccccgc atccacgttg atgtaggggt
     2521 ccatcttctg gagcgtgacc cggaagccgc gcgcctcgag cagggatccg atcgacgccg
     2581 cggccagccc cttgcccagg gaggacacga caccgcccgt cacgaagatg aatctggtcg
     2641 tcacctcacg cctcctttcc cgatgtgagc cattctttca cgagttcccg ctcctcggcc
     2701 accgagccca ccgctccgtc cagccggcac cgccttagcc gggcgagcgc ccggcccacc
     2761 tgcgggccgc gcggcatccc cagcgcgagg aggtctcccc cggagagccg cggctgcacc
     2821 gcccgcccgc tcgccaggta ccactcgatc cgccggcgcg ccgccgcgct cccgcggagc
     2881 cacgccgaga gcgcggccgc cggcggcacc gggcggagca cgtcgtccac cgcgctcgga
     2941 cgaagccgcg gggcgccgag cctccgggcc agcggccccg ccatggcccc cgcctcgagg
     3001 ttggcccgcg gctcgccgct cagcctgagg cgatcgagac agcgcgctac cgcggtcgcg
     3061 gagctcccgg cgaggagggc cagcatgaac agctccgccg ggtcgaccgc cactccggcg
     3121 gccctcgccc agcgcgcgag gcggtcggca tcggccagac ggccagtcga gacattgctt
     3181 attcccagga gcgttgcgac tctcgatctc accagatgct cgaaggcctg gcgggcgcac
     3241 ggctcggccg ccgccagctc gatctcgcgc cagaggcgct ggccggagag cgccacgtac
     3301 gaccgccggg
//
LOCUS       DATMCV010000562         2949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_497156, whole genome shotgun sequence.
ACCESSION   DATMCV010000562 DATMCV010000000
VERSION     DATMCV010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497156"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VNU03_12900"
     CDS             <1..319
                     /locus_tag="VNU03_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006089643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HWP75412.1"
                     /translation="GGRVTARFGLERDRSLAEPQLRTTRPPSSTAPGPWFATTAHGPD
                     LFAASQQAVRYMIDHLVRERGLSREEAYILCSVTVDLKISEIVDAPNWIVSAFLPEGV
                     FVA"
     gene            390..1280
                     /locus_tag="VNU03_12905"
     CDS             390..1280
                     /locus_tag="VNU03_12905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWP75413.1"
                     /translation="MSTPAVTPAPVATAPQAPFLGLSTLWVQITGTWCNLECRHCINA
                     SGPRSPWLKPLDGETIRRAIAEAEALGVKEIYFTGGEPFLHGEILPLLERALEAAPTT
                     VLTNGTLIGPEQADALAALAARALYSLEIRVSLDDPDRARNDAIRGEGSWEKAVEAVR
                     LLKARGLLPIVTATEITADAHPGPGRMHGRFRDFLREQGVERPRLKIMPVFALGRQPA
                     TGEPSLTEEDLEGFDRSMLQCADARVVAAGGVYACPILAGLPGARLSTGSLAESFRPV
                     PLYHPACVTCRRTGMSCRNS"
     gene            1265..2065
                     /locus_tag="VNU03_12910"
     CDS             1265..2065
                     /locus_tag="VNU03_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWP75414.1"
                     /translation="MPEQLGEYRRIAVLGGVYSNALALEATLADARARGVEALFCLGD
                     MGGFGPHPDRVFPLLREHGVLAIQGNYDESLASGRTDCGCGYTDPRDNHYAAISYRYT
                     FEGTSTENKTWLGALPRHRRIKLGPHRVLMCHGSPRVINEFLWESATPNGLLRHLMER
                     AEADVMLCTHTGIKWHRALPDARHAVNVGVIGRPENDGRTNVWYTVLTAGPDLAVEFV
                     PVAYNHEALAREMEGEGLPAEFAETVRTGWWTTCLENLPARERARGKY"
     gene            2113..2748
                     /locus_tag="VNU03_12915"
     CDS             2113..2748
                     /locus_tag="VNU03_12915"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HWP75415.1"
                     /translation="MARALGLADLIRLDNVAHLREWIDGFGAWAPAVFVGGYVVAVVA
                     FVPALPLTILGGLVFGPIEGIVYSSIASTAGAALAFLIARYAARDVVERWMAPHPALG
                     RMDRAVARHGWRIVMITRLVPIFPFNLQNYAYGLTSIGFRAYVVASIVCMLPATVAYT
                     LAAGAIVTGAWDVRRMVLLLGAAGLLLVALSLLPRWLRGRSAALDDLLRTG"
     gene            2748..>2949
                     /locus_tag="VNU03_12920"
     CDS             2748..>2949
                     /locus_tag="VNU03_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75416.1"
                     /translation="MRGAGLARLAAVLLGLGALVAGAGAAEPLRLGDFTPPADPAAPP
                     PGWEALTFPRVARHTRYTVVRDG"
BASE COUNT          358 a         1104 c         1067 g          420 t
ORIGIN      
        1 gggcggtcgg gtgaccgcgc gcttcgggct cgagcgcgac cggagcctcg ccgagccgca
       61 gctgcgcacc acgcggcccc cttcgtcgac cgcccccggg ccgtggttcg ccaccaccgc
      121 gcacggcccc gatctcttcg cggcctccca gcaggcggtg cgctacatga tcgaccacct
      181 cgtgcgcgag cgcgggctct cccgggagga ggcctacatc ctgtgcagcg tcacggtcga
      241 tctcaagatc agcgagatcg tggacgcgcc caactggatc gtctcggcct tcctgcccga
      301 gggtgtgttc gtcgcctgat ttgacgccgc tgtgacatcc tccgtcgcga ggcgcgcatg
      361 ctgggccaga tgatgcccga gcccgccgga tgagcacgcc cgccgtcacg ccggccccgg
      421 tggccacggc cccgcaggcg ccgtttctcg ggctctccac gctctgggtg cagatcaccg
      481 gcacctggtg caacctcgag tgccggcact gcatcaacgc gagcgggccg cgctcgccgt
      541 ggctcaagcc cctcgacggc gagacgatcc ggcgggccat cgccgaggcg gaggcgctcg
      601 gcgtgaagga gatctacttc accgggggcg agccgttcct gcacggcgag atcctgcccc
      661 tgctcgagcg cgcgctcgag gccgcgccga ccaccgtcct caccaacggc accctcatcg
      721 gacccgagca ggccgacgcg ctggccgcgc tggccgcccg cgcgctctac tcgctcgaga
      781 tccgggtgag cctcgacgac ccggaccggg cgcgcaacga cgcgatccgc ggcgaggggt
      841 cgtgggagaa ggcggtggag gcggtgcgcc tcctgaaggc gcgcggcctc ctgcccatcg
      901 tgaccgccac cgagatcacg gccgacgcgc accccgggcc gggccgcatg cacgggcgat
      961 tccgcgactt cctccgcgag cagggcgtcg agcgcccgcg gctcaagatc atgccggtgt
     1021 tcgccctcgg ccggcagccc gcgaccggcg agccgtcgct caccgaggag gatctcgaag
     1081 gcttcgaccg ctcgatgctc cagtgcgcgg acgcgcgcgt ggtggcggcg ggcggcgtct
     1141 acgcgtgccc gatcctggcc ggcctcccgg gcgcgcggct ctccaccggc agcctcgcgg
     1201 aatccttccg gcccgtgccc ctctatcacc cggcgtgcgt gacctgccgc cggacgggaa
     1261 tgtcgtgccg gaacagctag gcgagtaccg gcgcatcgcg gtcctcgggg gcgtgtacag
     1321 caacgcgctc gcgctcgagg ccacgctggc cgacgcgcgg gcgcgcgggg tggaggcgct
     1381 cttctgcctg ggcgacatgg gcgggttcgg gccgcacccg gaccgcgtgt tccccctgct
     1441 gcgcgagcac ggggtgctcg ccatccaggg caactacgac gagtcgctcg cctccgggcg
     1501 caccgactgc ggctgcggct acaccgaccc gcgcgacaac cactacgccg cgatcagcta
     1561 ccggtacacc ttcgagggca cgtcgaccga gaacaagacg tggctcgggg cgctgccccg
     1621 gcatcgccgc atcaagctcg gcccgcaccg ggtgctgatg tgccacggct cgccgcgggt
     1681 gatcaacgag ttcctgtggg agtcggccac cccgaacggc ctcctgcgcc acctgatgga
     1741 gcgggccgag gccgacgtca tgctgtgcac gcacaccggg atcaagtggc accgcgcgct
     1801 gccggacgcc cgccacgcgg tcaacgtggg cgtgatcggc cggcccgaga acgacggccg
     1861 caccaacgtc tggtacacgg tgctgaccgc gggtcccgat ctcgcggtgg agttcgtgcc
     1921 ggtcgcctac aatcacgagg cgctggcccg cgagatggag ggggaggggc tgcccgccga
     1981 gttcgcggag acggtccgca ccgggtggtg gaccacctgt ctcgagaacc tgcccgcgcg
     2041 cgagcgggcg cgaggcaagt actgagcaag cgactgctgc tggtcggcct gatcgcggtc
     2101 gcactggtgg cgatggcgcg ggcgctcggg ctggccgacc tgatccggct cgacaacgtc
     2161 gctcacctgc gggagtggat tgacgggttc ggggcctggg cccccgcggt cttcgtgggc
     2221 ggctacgtgg tcgcggtggt cgcgttcgtc ccggcgctgc cgctcaccat cctgggcggg
     2281 ctcgtcttcg gcccgatcga gggcatcgtg tacagctcga tcgcctccac cgcgggggcg
     2341 gcgctcgcct ttctcatcgc gcgctacgcc gcgcgcgacg tggtggagcg ctggatggcg
     2401 ccccatcccg cgctcggccg catggaccgc gcggtggccc ggcacggctg gcgcatcgtg
     2461 atgatcaccc ggctggtgcc gatcttcccg ttcaacctcc agaactacgc gtacggcctc
     2521 acctcgatcg gcttccgcgc ctacgtggtg gcctccatcg tgtgcatgct gcccgcgacg
     2581 gtggcctaca cgctggcggc gggggccatc gtgaccggcg cgtgggacgt gcggcgcatg
     2641 gtgctgctgc tgggcgcggc cgggctgctc ctcgtcgcgc tctcgctgct gccgcgctgg
     2701 ctgcgcgggc gcagcgccgc gctcgacgac ctgctgcgga ccggctgatg cggggcgccg
     2761 ggctcgcgcg cctggccgcg gtcctgctgg ggctgggcgc gctcgtcgcg ggggcggggg
     2821 cggccgagcc actccggctg ggcgacttca ccccgcccgc cgatccggcc gcgccgccgc
     2881 cgggctggga ggcgctcacg ttcccccgcg tcgcgcgcca cacccgctac accgtcgtgc
     2941 gcgacgggc
//
LOCUS       DATMCV010000563         8897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_693049, whole genome shotgun sequence.
ACCESSION   DATMCV010000563 DATMCV010000000
VERSION     DATMCV010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693049"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VNU03_12925"
     CDS             <1..388
                     /locus_tag="VNU03_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWP75417.1"
                     /translation="MSLVGPRPLLMEYLPRYTREQARRHDVRPGITGWAQLNGRNTLP
                     WERRFALDVWYVDHQSFRLDTRILVLTVWHALAQVGVSQPGEGTEEHFRGCEPATDAP
                     ASSESASHMPASKMPASNMEEVHRWS"
     gene            379..1497
                     /locus_tag="VNU03_12930"
     CDS             379..1497
                     /locus_tag="VNU03_12930"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWP75418.1"
                     /translation="MELMPIIRPTLPEFREFEELVRPSWDEGQVTTGRIGRAFEEEIC
                     RQTGAREAVSMSSCTAGLMLAAQALELPAGAEVIVPSFTFAATAQALVWNGLVPVFCD
                     CRPDTLTLDPADVERNLTPRTAAICAAYVYGLPPDIEGLTALGERHGVPVYFDSAQGL
                     GAEYQGRKAGVFGRCEIFSLSPTKVVTAIEGGVICTGDPGLAARLRSMRDYGKDPQKG
                     EDMVHLGLSARMSELHAAVGLLSLRRVDELVKARRQLIEIYRARLGALPGCGVQSVPP
                     DRSSSGNYFVLRITAGARAGRDAVYAGLAARGIQTKRYFYPPVHEQTLLRRVPHRVSE
                     RMEHTVTASRESLALPLYSHMTPEQVDRVCREVECLLA"
     gene            1514..2872
                     /locus_tag="VNU03_12935"
     CDS             1514..2872
                     /locus_tag="VNU03_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWP75419.1"
                     /translation="MAMPTRLSAVTTAARSGLALSAAPGDSIARHFAIGAFWALVGAV
                     VSRGLTLASSVVAGRLLGTTGFGEIGMIQSTQGLFGIVAGAGLGLAATKFVAEFRSTD
                     PARTGRCATLATTIASVSGTVMALVLLALSGVMASSVLRAPHLTVELQVATGLVLFGT
                     INGVQTGALVGFGDFRTLAVLSGIRGLCLCALLIVGITLGGVLGGVIGLVLTEAIAVL
                     ANHVALRRVLPATVTWRAGRAAWSELALMCRFSGLSLLGSICTMSAMWFANLMLVAQP
                     DGYASLGVFNAAERWRQLLLFLPASFSPVILTSLSNLHGRNDPDAYRRLFGLNLAVSV
                     AVVVIPSIGIVLWAPSAMGLFGDEYRAGGMTLVILAASSVVVVLNNLLGQVLVSRGAM
                     MGRLVLDVLLAAVLALASWQLIPLYREQGMALGSLIAFAVTSAALVVTTGYFMRAHRS
                     GR"
     gene            2869..4293
                     /locus_tag="VNU03_12940"
     CDS             2869..4293
                     /locus_tag="VNU03_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75420.1"
                     /translation="MRASRNPLPGATFWVLAIDALALIAFVCAPMAQEYPLAAAGILA
                     FMFVQVALPACRIRVTAPLCPANIAQGYYWIQLVLVPVLVGYYGFSQGVLPHLPSKGA
                     IDLAIVVHVVSYLAFCVAYQCFGRATVRRGRGPGPTPGTAYLIVPFAVVGVVGFLLAH
                     GGLGGFVDYVSSPMESRLRAEEATTLQGAAGTFLKHFLGFAVVLAWSWWLGKAPRRKA
                     AIIAATAAVVMILLVVNFNYNRGTMLGPILALAAAFSVHVWRIPFKGVVLAGVLALSI
                     AFVFGAYRATNLEITEMSASAVADTWNAEGVVEFVQIYASAPQMSAYLIEALEGQFYY
                     GKTLLPSVVYPIPVLGKPFRDISGPVIFNELIYGDSDSVDQIIPLAGELYINFHLAGV
                     VLGGMLLGWLLAWQQGRFMTAPTPVEGYAWLMMALWTVFPGSLSVASQIYLYSFWPIY
                     VYFAATRLWSWNAAMARHRDRRFA"
     gene            4248..5387
                     /locus_tag="VNU03_12945"
     CDS             4248..5387
                     /locus_tag="VNU03_12945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP75421.1"
                     /translation="MECRDGPPPRPAVRMNICLVGGIFDRPEAVRSKQLVTPETVLLD
                     GFRKAGVTVHAVGHAGFQPSDDYDIIHVHHFGRAALKMASSRCRARVVFTGHNGLIPT
                     GWERSRLRRYAFQYVVDRAEAYVALSRAEARYYARVTSADKIHTIPNGIPADVFRAAG
                     HRGKRADKERYDVLYVGQLIDWKGVNFLLEAMQRLRRQRDVHLRLVYHNAELEADLRR
                     QADDLGIAKHVEFVGILDPRELAEEYQQADLLVLPSFADCLPSVVTEAFLCGTPVVAG
                     GVCGVPEQIGPYGLAVPPGDSVALADAMESILADRERWQGLAAEIRAYAEHTFKPETM
                     VARHLALYRDMVEGRAASVPRRAAWIDPAMRLAVEVYWRHPRAAA"
     gene            5398..6513
                     /locus_tag="VNU03_12950"
     CDS             5398..6513
                     /locus_tag="VNU03_12950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP75422.1"
                     /translation="MNISHISVSTLPVLHRFGGAIERRIVEIAREQARRGHRVGVYSV
                     GDTDETREVDGVTYHFLKCRTRLPFKHLEFQYRVARELQKGSDDVLHFHSQPEGAWMS
                     HAVGARKVLSYDFFQFRGGRQTPLYHLYKRALRSYDLLLPCSRYCLEESQTFWSFPTS
                     KLRILHNGVNTRQFRPDPEAAARERAQLGIDKRVMLYVGRVCEQKGSDVLLRAARTLR
                     ERRRDLQLVIAGPIGQFGLRDDPDGWVGRIAEAGGLYLGPVEEGRLNAIYNLADVFVM
                     PTRVNEMFGMAAVEAQACGKPVVSSDSGGLREVVPSSCGARVPVGDASGMADEVEKLL
                     DDGRLYATCSANARLNAATYDWARICDTCDDLYRVAS"
     gene            6529..7467
                     /locus_tag="VNU03_12955"
     CDS             6529..7467
                     /locus_tag="VNU03_12955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWP75423.1"
                     /translation="MSERVSVTIGIPFRNARRSLATAVRSVFAQTHHDWELLLIDDGS
                     TDGSIEAVRRLDDPRVRLLADGSHRGLCARLNQIAAMARGTYLARMDADDVMHPERVE
                     RQLALLQTDPSVDVVDTATFTMDDDLTPLGVRGDRPLDCRPESVLRDGLLIHPTVMGR
                     AEWFRRHPYDPVFVRAEDRELWIRTCTTTRFARLGRPLFFYREGPGGNLENYLRTERT
                     LRDILRRYGPPLVGVRRTRLLVMRSRLKSIAYRLGTRLGQQARLISRRNRRLEASEVR
                     EARLILSAIQGTPVPGLDNDAPAPSAERRLPGEAAA"
     gene            7464..8675
                     /locus_tag="VNU03_12960"
     CDS             7464..8675
                     /locus_tag="VNU03_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP75424.1"
                     /translation="MTPRLLHVTTVPMTLRFVVGHVAHARSRGFEVHVLSSPGEALDE
                     FARDVQVEAHAVPMARRIAPLADLAALWRMWRVMRHVRPTIVDAHTPKGGLLAMLAAA
                     LCRVPVRVYHQHGLPLMTATGLKRRILRWTERIACQLAHQVICISPLLREVLIKEGLC
                     PPGKITVLEHGSIDGVEADGKFDPAGVSTQWAELVRSRYQIPPGAPVIGFVGRVVRDK
                     GLIELAQAWRVLREEWPSLHLLVAGPFESEDPIPADVEATLRGDPRVHLAGMVRDMPG
                     IYRTLDLLVLPSYREGFNTALLEAAAMGLPVVATRISGCVDAVRDGETGLLVPVRDAQ
                     ALIDAIGRYLGDPALRRRHGANGRRRALHEFDPGRLREALFQEYVRLLGRRGCDEMVA
                     HVGRAPGEAAR"
BASE COUNT         1180 a         3013 c         3219 g         1485 t
ORIGIN      
        1 catgagcctg gtcggccccc ggccgctcct gatggagtac ctgccgcgct acacgcggga
       61 gcaggcccgc cgccacgacg tgcggcccgg gatcaccggg tgggcccagc tcaacggccg
      121 caacaccctg ccgtgggagc ggcggttcgc gctcgacgtc tggtacgtcg accaccagtc
      181 gttccgcctc gacacccgga tcttggtatt gacggtctgg cacgcgctcg cgcaggtcgg
      241 cgtcagtcag cccggggagg ggaccgagga gcatttccgc gggtgcgagc cggcgacgga
      301 cgcgcccgcg tcgagcgagt ccgcgtcgca catgcccgcg tcgaagatgc ccgcatcgaa
      361 tatggaggag gtccaccgat ggagctgatg ccgatcattc gcccgaccct gccggagttc
      421 cgcgagttcg aggagctcgt gcgtcccagc tgggacgagg gccaggtcac gacgggacgg
      481 atcggacgcg ccttcgaaga ggagatctgc cggcagaccg gcgcgcgcga ggcggtctcg
      541 atgtcgtcgt gcaccgcggg gctcatgctg gccgcccagg cgctcgagct gccggcgggc
      601 gccgaggtca tcgtgccctc cttcacgttc gcggccaccg cgcaggcgct ggtgtggaac
      661 ggcctggtcc cggtcttctg cgactgccgg ccggacaccc tgacgctcga tccggccgac
      721 gtcgagcgga acctgacgcc gaggaccgcc gcgatatgcg cggcgtacgt ctacgggctc
      781 ccgccggaca tcgaggggct gaccgcgctc ggcgagcggc acggcgtccc ggtgtatttc
      841 gacagcgccc agggactggg cgccgagtac cagggccgga aggcgggcgt cttcggacgg
      901 tgcgagatct tctcgctgag cccgaccaag gtcgtcaccg cgatcgaggg cggcgtgatc
      961 tgcaccggcg atcccggcct ggccgcgcgc ctgcggtcga tgcgggacta cggcaaggat
     1021 ccacagaagg gcgaggacat ggtccacctc ggcctctccg cccggatgag cgagctgcac
     1081 gccgcggtgg gcctgctctc gctgagacgc gtggacgagc tggtgaaggc gcggcggcag
     1141 ctgatcgaga tctaccgggc gcggctcggc gcgctgcccg gatgcggcgt gcagagcgtc
     1201 cctccggacc ggtcgagcag cggcaactac ttcgtgctgc gcatcaccgc cggggcccgg
     1261 gccggccggg acgcggtgta cgcggggctc gcggcgcgcg ggatccagac caagcgctac
     1321 ttctatcctc cggtgcacga gcagaccctc ctccggcggg tgccgcatcg ggtgagcgag
     1381 cggatggagc acaccgtgac cgcgagccgg gaatccctgg ccctgcccct gtactcgcac
     1441 atgacgccgg aacaggtcga ccgcgtgtgc cgggaggtcg agtgcctgct ggcctgatgc
     1501 cggccggggg cccatggcga tgccgacgcg tctctcggcc gtgaccaccg ccgccaggag
     1561 cgggctggcc ctctcggccg ccccgggcga ctcgatcgcg aggcatttcg cgatcggcgc
     1621 gttttgggcg ctggtgggcg cggtcgtgtc gcgcggcctg acgctggcgt cgtcggtggt
     1681 ggcgggccgg ctcctgggca cgacgggctt cggcgagatc ggcatgatcc agagcacgca
     1741 gggcctgttc gggatcgtgg ccggcgccgg gctggggctg gccgcgacca agttcgtggc
     1801 ggagtttcgc tcgaccgatc ccgcgagaac cgggcgctgc gccaccctgg ccaccaccat
     1861 cgcctccgtc tccggcaccg tgatggcgct ggtgctcctc gccctttcgg gggtgatggc
     1921 gtcgagcgtg ctgcgcgccc cccacctgac cgtggagctg caagtggcga ccgggctggt
     1981 cctcttcggc acgatcaacg gcgtgcagac gggcgctctc gtcggcttcg gcgactttcg
     2041 gaccctcgcg gtcctgagcg gcatccgcgg cctctgcctg tgcgcgctcc tgatcgtcgg
     2101 catcacgctc ggcggcgtcc tgggcggagt catcggcctg gtcttgaccg aggccatcgc
     2161 ggtgctcgcc aaccatgtcg ctctgcggcg cgtccttccc gcgacggtca cctggcgagc
     2221 cggccgcgcc gcctggagcg agctggcgct gatgtgtcga ttcagcgggc tgagcctgct
     2281 ggggagcatc tgcacgatgt cggcgatgtg gttcgccaac ctgatgctgg tcgcgcagcc
     2341 cgacggctac gcctcgctcg gcgtcttcaa cgcggccgag cgctggcggc agctgctgct
     2401 gttcctcccc gcctcgttct cgccggtgat cctcacgagc ctgagcaacc tgcacggccg
     2461 gaacgatccg gacgcctatc gccggctctt cggtctcaac ctcgcggtca gcgtcgcggt
     2521 cgtcgtgatc ccgtcgatcg gcatcgtgct gtgggcgccg tcggcgatgg gtctcttcgg
     2581 cgacgagtat cgcgccggcg gcatgaccct ggtcatcctg gccgcctcgt cggtggtggt
     2641 cgtgctcaac aacctgctgg ggcaggtcct ggtgagccgg ggggccatga tggggcgcct
     2701 cgtcctcgac gtgctgctcg ccgcggttct cgccctcgcg tcgtggcagc tgattccgct
     2761 ctatcgcgag caggggatgg cgctgggcag cttgatcgcg tttgcggtga cctcggccgc
     2821 tctggtcgtg acgacgggct acttcatgag agcgcatagg agcgggcgat gagagcctcg
     2881 aggaacccgc tgcccggcgc caccttctgg gtgctggcga tcgacgcgct cgccctgatc
     2941 gcgttcgtct gtgcgccgat ggcgcaggag tatccgctcg ccgccgccgg cattctcgcg
     3001 ttcatgttcg tgcaggtggc cttgccggcc tgtcggatcc gcgtgaccgc ccccctgtgc
     3061 ccggccaaca tcgcccaggg ctactactgg atccagctgg tcctcgtccc ggtcctggtc
     3121 ggctactacg ggttcagcca gggggtgctg ccacatctgc cgtcgaaggg tgcgatcgac
     3181 ctggccatcg tcgtgcacgt ggtgagctac ctggccttct gcgtggcgta ccagtgcttc
     3241 ggccgcgcga cggtgaggcg cggtcggggt cccggcccga ccccgggcac cgcctacctg
     3301 atcgtgccgt tcgcggtggt gggagtcgtg gggttcttgc tggcgcacgg cggcctcggc
     3361 gggttcgtcg actacgtctc gtcgccgatg gaatctcgcc tgcgggcgga ggaggcgacg
     3421 acgctccagg gggcggccgg caccttcctc aagcattttc tcggcttcgc ggtggtgctg
     3481 gcgtggagct ggtggctggg caaggcgccg cgccggaagg cggcgatcat cgccgcgacg
     3541 gccgcggtcg tgatgatttt gttagtggtc aatttcaact acaaccgggg cacgatgctc
     3601 ggaccgatcc tcgccctggc cgccgcgttc tcggtgcacg tgtggcgcat tccgttcaag
     3661 ggcgtggtcc tggccggcgt cctcgcgctg tccattgcgt tcgtgttcgg cgcgtatcga
     3721 gccaccaatc tcgagatcac cgagatgtcg gcctcggcgg tcgcggacac atggaacgcc
     3781 gagggcgtcg tcgagttcgt gcagatctac gcctcggcgc cgcagatgtc cgcctacctg
     3841 atcgaggccc tcgagggcca gttctactac ggcaagaccc tgctcccctc ggtggtctac
     3901 ccgattccgg tgctgggcaa gccgtttcgc gacatcagcg gccccgtgat cttcaacgag
     3961 ctgatctacg gcgactcgga cagtgtcgat cagatcattc cgctggccgg cgagctctac
     4021 atcaacttcc acctggccgg ggtcgttctc ggcggcatgt tgctcggttg gctgctcgcc
     4081 tggcagcagg gcaggttcat gaccgctccg accccggtcg aaggctacgc ctggctcatg
     4141 atggcgctct ggaccgtgtt cccgggcagc ctgtcggtcg cgagccagat ctatctctac
     4201 tcgttctggc cgatctacgt ctacttcgcg gccacgcggc tctggtcgtg gaatgccgcg
     4261 atggcccgcc accgcgaccg gcggttcgca tgaacatctg cctggtgggc ggcatcttcg
     4321 atcgaccgga ggcggtccgc tccaagcagc tggtgacgcc ggagacggtg ctgctcgacg
     4381 gctttcgcaa ggccggagtg acggtgcacg cggtcggcca cgccggcttt cagccgtcgg
     4441 acgactacga catcattcac gtccatcatt tcggtcgggc ggccctcaag atggcgtcct
     4501 cgcgctgccg ggcgcgcgtc gtcttcaccg gccacaacgg gctgatcccc accggctggg
     4561 agcgctcgcg gctgcggcgc tacgccttcc agtacgtggt ggaccgggcg gaggcctacg
     4621 tcgcgctgtc gcgcgccgag gcccggtact acgcccgggt gacgagcgcc gacaagatcc
     4681 acaccattcc caacggcatt ccggccgacg tctttcgcgc cgccggacat cgggggaagc
     4741 gagccgacaa ggagcgctac gacgtgctct acgtcggcca gctgatcgac tggaaggggg
     4801 tcaacttcct gctcgaggcg atgcaacggc ttcgccggca gcgcgacgtt catctccggc
     4861 tggtctatca caacgcggag ctggaggcgg acctcagacg gcaggccgac gacctgggca
     4921 tcgccaagca cgtggagttc gtgggcatcc tcgatccgag agagctggcc gaggagtatc
     4981 agcaggcgga cctgctggtg ctgccgagct ttgccgactg cctgcccagc gtggtcaccg
     5041 aggcgttcct gtgcggcacg ccggtggtgg ccggcggggt gtgtggcgtg ccggagcaga
     5101 tcggacccta cggcctggcg gtgccgccgg gcgattcggt cgcgctcgcg gacgccatgg
     5161 agtcgatcct ggcggatcgg gagcgctggc agggcctggc ggccgagatc cgggcgtacg
     5221 cggagcacac gttcaagccc gagaccatgg tcgcccggca cctcgccctc tatcgcgaca
     5281 tggtcgaagg ccgcgccgcg tcggtcccgc ggcgggccgc ctggatcgat ccggcgatgc
     5341 gcctcgcggt cgaggtgtac tggcgccacc ctcgggccgc ggcctaggac ggacgcgatg
     5401 aacatcagcc acatcagcgt cagcacgctg ccggtcctgc atcgcttcgg cggcgccatc
     5461 gagcggcgca tcgtcgagat cgcgcgcgag caggcgcggc gcgggcatcg ggttggcgtg
     5521 tactcggtcg gtgacaccga cgaaacgcgc gaagtggacg gcgtgaccta tcactttctg
     5581 aagtgccgca cgcgtctgcc gttcaagcac ctcgagttcc agtaccgggt cgcgcgcgag
     5641 ctccagaagg gctccgacga cgtgctgcac ttccacagtc agcccgaggg cgcgtggatg
     5701 agccacgcgg tgggggcccg gaaggtgctc tcgtacgact tcttccagtt ccgcggtggc
     5761 cgccagacgc cgctctatca cctgtacaag cgggcgctcc gctcctacga cctgctcctg
     5821 ccgtgctcgc ggtactgcct ggaggagtcc cagaccttct ggagctttcc cacgagcaag
     5881 ctgcggatcc tccacaacgg ggtcaacacg cggcagttcc ggcccgatcc ggaggcggcc
     5941 gcgcgcgagc gcgcccagct cggcatcgac aagcgggtga tgctctacgt gggccgggtc
     6001 tgcgagcaga aggggagcga cgtgctgctc cgggcggccc ggacgctccg cgagcgccgt
     6061 cgcgacctgc agctcgtgat cgcgggaccg atcgggcagt tcggcctccg ggacgatccg
     6121 gacggctggg tcggacgcat cgcggaggcc ggcggcctct acctcggccc ggtcgaggag
     6181 ggacggctca acgcgatcta caacctggcc gacgtcttcg tgatgccgac ccgggtcaac
     6241 gagatgttcg gcatggcggc ggtcgaggcg caggcgtgtg gcaagcccgt cgtctcgagc
     6301 gactcgggcg gcctgaggga ggtggtcccg tcgagctgcg gcgcgcgcgt tccggtcggc
     6361 gatgcgtccg ggatggcgga cgaggtcgag aagctgctgg acgacggccg cctctacgcg
     6421 acctgctccg cgaatgcgcg gctcaacgcg gcgacctacg actgggcccg catctgtgac
     6481 acgtgcgacg acctgtaccg ggtcgcgagc tgaagcctgg agagccgcgt gagtgagcgc
     6541 gtgtccgtca ccatcggcat cccgttccgg aacgcccggc gctccctggc caccgcggtg
     6601 cgctcggtgt tcgcgcagac ccaccacgac tgggagctgc tcctgatcga cgacggctcc
     6661 accgacggct cgatcgaggc ggtgcggcgt ctcgacgatc cgcgggtgcg gctcctcgcc
     6721 gatgggagtc atcgcggtct gtgcgcgcgc ctgaaccaga tcgcggcgat ggcccggggc
     6781 acctacctgg cgcggatgga cgccgacgac gtcatgcatc cggagcgggt ggagcggcag
     6841 ctcgcactgt tgcagacgga cccgagcgtc gatgtcgtcg acactgcgac cttcacgatg
     6901 gacgacgacc tcacgccgct gggcgtacgg ggcgatcggc cgctcgattg ccgtccggag
     6961 tcggtgctgc gcgacggact cctcatccat ccgacggtca tgggacgcgc cgagtggttt
     7021 cgccgccatc cgtacgatcc ggtcttcgtt cgggccgagg accgcgagct gtggatccgg
     7081 acctgcacga cgaccaggtt tgcccggctg gggcggccgc tgttcttcta tcgcgaagga
     7141 ccgggcggca acctggagaa ctacctgcgg accgagcgga ccctgcgcga catcctgcga
     7201 cgctacgggc cgccgctggt cggggtgcgg cgcacccggc tcctggtgat gcggtcacga
     7261 ctcaagtcga tcgcctaccg cctcggcacg agactcgggc agcaggctcg gctgatcagc
     7321 cggcgcaacc gccggctcga agcgtcggaa gtacgggagg cgaggttgat tctctctgcg
     7381 atccagggga cgccggtgcc gggactggac aacgacgcgc cggccccctc ggccgagcga
     7441 cggcttccgg gcgaggcggc cgcgtgaccc cccggctgct gcacgtgacg acggtgccga
     7501 tgaccctgcg cttcgtggtg gggcacgtgg cccatgcccg gagtcggggg ttcgaggtcc
     7561 acgtgctctc gtcgcccggc gaggcgctcg acgagtttgc tcgcgacgtg caggtcgaag
     7621 cgcacgcggt gccgatggcg cggcggatcg cgccgctggc cgatctcgcg gcgctgtggc
     7681 ggatgtggcg agtcatgcgg cacgtgcggc cgacgatcgt ggatgcgcat acgcccaaag
     7741 ggggcctcct cgcgatgctg gcggccgcgc tctgccgggt gccggttcgc gtctatcacc
     7801 agcacgggct gccgctcatg accgccaccg ggctgaagcg gcgtatcctc cgctggaccg
     7861 agcgcatcgc gtgccagctg gcgcaccagg tcatctgcat cagtccgttg ctgcgcgagg
     7921 tcctgatcaa ggagggcctg tgcccgcccg gaaagatcac ggtgctcgag cacggaagca
     7981 tcgacggcgt cgaggcggat ggcaagttcg atccggccgg ggtctcgacg cagtgggcgg
     8041 agctcgtgcg gtctcgctat cagattccac cgggcgcgcc ggtgatcggc ttcgtgggtc
     8101 gcgtcgtccg cgacaagggc ttgatcgagc tcgcgcaagc ctggcgggtg ctgcgtgagg
     8161 agtggccctc gctgcacctg ctggtcgcgg ggccgttcga gagcgaggat ccgatcccgg
     8221 ccgacgtgga agcaaccctg cgaggcgatc cgcgcgtcca tctggccgga atggtgcgcg
     8281 acatgcccgg gatctatcgg acgctggacc ttctggtcct gccgtcgtat cgcgagggct
     8341 tcaacaccgc gctcctggag gcggcggcga tggggctgcc ggtggtcgcg acccggatct
     8401 cgggatgcgt cgatgcggtc cgggacggcg agaccggact gctggtgccg gtgcgcgacg
     8461 cccaggccct gatcgacgcg atcggacggt acctgggcga ccccgcgctg cgtcgacgcc
     8521 acggcgccaa cggccggcgc cgcgcgctgc acgaattcga cccgggaagg ctccgcgagg
     8581 ccttgtttca ggagtacgta cgcttgctgg gccggcgcgg atgcgacgag atggtggccc
     8641 acgtgggccg ggccccgggg gaggcggctc gctagtgtgg cccgcgctgc cgttcatggc
     8701 gttcgtggcg gtcttgccct ttcgaggaac catgaccttg cggctggtct gtctcgccgc
     8761 ggcgggcctg atcgtcgcga cgtcgtggcg gcggctgaac ccgccggcct tgccctgccg
     8821 gtggacgatc gcgctctggg cgggggtcgt gctgggctcg cttctcgtcg cggtcgatcc
     8881 ggcctacagc gtgcggg
//
LOCUS       DATMCV010000564         5670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_27974, whole genome shotgun sequence.
ACCESSION   DATMCV010000564 DATMCV010000000
VERSION     DATMCV010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27974"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1100)
                     /locus_tag="VNU03_12965"
     CDS             complement(81..1100)
                     /locus_tag="VNU03_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75425.1"
                     /translation="MSAAARLALACLLAVPVWTTASSLVATSTFLVEFLGQGRWRPLS
                     ALTREPLTRPLAAPPSPAGPVAIDLYVRPALRRAPGLVLVHGLSPEGKDDARLREAAG
                     QLARAGWAVAVPTVRGLTALRLRPDDAGAVAASVQALRAAGYPRVAILGVSLGAGPAL
                     LAAAESGLAGDVSAVLALGGYASAVELLRYTLTGAYAFAELSGRASVHEEAIAQFARA
                     NTELVDGAGRRLVDNRDPVAFDGLVDALPRPTREMLDALSPGRVLGGLHAPLFLVHGR
                     QDPAVPFTESLRLRTAALAAGRPVRAAIVGAVGHVEAGSHASNLADLARLWATLYAFR
                     RTAES"
     gene            1339..1827
                     /locus_tag="VNU03_12970"
     CDS             1339..1827
                     /locus_tag="VNU03_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75426.1"
                     /translation="MSVTWGRILVGIAVAMCLLGVGVLEVLSERGESRVLPEPSRIGE
                     VRPEPWGEFGRVDPALTADADVSTPAASQGMTGAVQHRRLTILEVNERAGRLISLTGA
                     GRVLVTDLSRDPFVVTEDSGAGSLALLKPGDVVRIEPMSGRTQRIVVLRHGWQELESP
                     EK"
     gene            complement(1834..2463)
                     /locus_tag="VNU03_12975"
     CDS             complement(1834..2463)
                     /locus_tag="VNU03_12975"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP75427.1"
                     /translation="MDAALEKRQVKRAYKLYAPAYDFVFDWIFHPGREAAIRLLDIKR
                     GDRVLEVGIGTGLNLPLYPSHCHLTGIDLSEEMLEKAQDKVVELALGNVTLKTMDATT
                     MDFGQGEFESAVATYTISAVPDPVGVLREMKRVVRPGGNIVVLNHFRSERPAVGRLED
                     LVAPVCTRLGWKSNLPLEPLLKQVGLVPDITAKVNLFNGWRLIRCVNPK"
     gene            complement(2592..3755)
                     /locus_tag="VNU03_12980"
     CDS             complement(2592..3755)
                     /locus_tag="VNU03_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75428.1"
                     /translation="MPSRKRIVVLHLAGRYPLGGIGWQAIHYVLGLTRLGHDVYYVED
                     SGAHPYDPRVKSVVGNAAYSVAFLAEVMGRFGLADRWSYVDAVTSAHHGLSRERLSDL
                     FRDADALFNVCGATRLTEEHLRCPIRVYVETDPVFEQIRVAEGDREAILALEDHTHHF
                     TYGENLGQPDSPIPLEKFAWRPTRPPVIPDLWSTPTDPAAERFTTIATWKNAGKDIHF
                     RGETYLWSKHLNFLRVMDLPSRTRQALEMALEVEDPAMRDLLARNGWGLQEAFQVSRD
                     LTTYQRYIQQSRGEFTVSKDLVARTRSGWFSDRSVCYLAAGKPVVTQDTGFGKYIPAG
                     VGLLAFEGVEEAAAAIDEVNRDYERHSREARRIALEYFGSDQVLGQLCQEADL"
     gene            complement(3742..5526)
                     /locus_tag="VNU03_12985"
     CDS             complement(3742..5526)
                     /locus_tag="VNU03_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75429.1"
                     /translation="MSSFAGRVSGYLWPHRGRILWALAQVCLTTALELLKPWPIKFII
                     DSVLGGQPTPGGLGGGWSPGALLLTACIALVLIYAALGSLTVLTNYTTIRIGQRMVRD
                     LRSDLYAHLHRLSMAFHSRAQAGDLLYRVTADTFALQALTMNCLFPATTALILLGGMA
                     AIMFHLDRELTFLALGVCPLLLLAIGRLNRRITVAAGEMRERESEVYGVVQRAMQAMR
                     VIQAFTREDEEHRRFMSASERSLDAGLRLYTLQTFYSGVVNLVIALGTAAVVWVGARH
                     VLDGSLTVGSLMVFISYLASLYGPINNMFQVYGLAQSARVGVQRVLDTMDVERDVADG
                     SRSFPPEGARGEVIWEGVDFQYAPGVPILRGVDLHVTPGQRVAVVGPTGAGKSTLLSL
                     LPRFYDPTRGRVLVDGVDAREYRLQSLRRQVAMVLQPPLLFPVSIRENIAFGRPGARL
                     EEIVDAAKAARIHDMVARLPQGYDTVVGEHGVTLSEGEKQRLTIARAILRDSPILILD
                     EPTSALDPETERLIIEALEQLQVGRTTFIIAHRLSTVRMADVVVVIKDGEVAEQGSLE
                     TLLARPGPFAAMYGARATDRQADPLAIP"
BASE COUNT          868 a         1952 c         2058 g          792 t
ORIGIN      
        1 cgactaccgc ccggtgcgtg acgccatgat gctgatggac ttgcgcccgc ccaagtaggc
       61 cgccgcctgg cgcgcccggg tcagctctcc gcggtccgcc ggaaagcgta caaggtcgcc
      121 cacaggcggg ccagatccgc gaggttactc gcgtgcgagc cggcctccac gtgtcccacc
      181 gcgccgacga tcgcggcgcg gaccggccgg ccggccgcca gcgcggcggt ccggaggcgg
      241 aggctttcgg tgaaggggac cgccgggtcc tggcggccgt ggaccaggaa gagcggcgcg
      301 tggaggcctc cgaggacccg gccgggcgag agcgcatcga gcatctcgcg agtgggccgc
      361 ggcagggcat cgacgagccc gtcgaaggcc accggatccc ggttgtcgac gaggcggcgg
      421 cccgccccgt ccaccagctc ggtgttggcc cgggcgaact gggcgatggc ctcctcgtgc
      481 accgacgcgc ggcccgagag ctccgcgaag gcgtaggcgc cggtcagggt gtagcgcagc
      541 agctccaccg ccgaggcgta gccgccgagc gcgagcaccg ccgacacgtc ccccgccagg
      601 ccggactccg ccgccgcgag cagcgcgggc cccgcgccca ggctgacgcc caggatggcc
      661 acgcgcgggt agccggcggc ccggagcgcc tgaacgctcg cggcgaccgc gccggcgtcg
      721 tcggggcgta ggcgcagcgc ggtgaggccg cgcaccgtgg gcaccgccac cgcccagccc
      781 gcgcgcgcga gctggcccgc ggcctcgcgc aggcgcgcgt cgtccttgcc ctcgggcgag
      841 agcccgtgca ccaggaccag cccgggggcg cgccggagcg cggggcgcac gtagaggtcg
      901 atcgcgaccg gacccgccgg cgagggggga gcggcgaggg ggcgcgtgag cggctcccgg
      961 gtcagcgcgg agaggggtcg ccagcgcccc tggccgagga actcgaccag gaacgtgctc
     1021 gtggcgacca gggacgacgc cgtcgtccag accggcaccg cgagcaggca ggccagcgcg
     1081 agccgcgccg cggcgctcac cgctcggctc tcgggtctgg ccgggcgttc acgcgccccc
     1141 gattcacccg cctatgatac gtcgggcaat gtgccacccg gccttcaggc aaggtgccca
     1201 ctttcccagc gcccggcaga ccgcatcgca gaatgtgcgc atcgttccag cacgttaccg
     1261 agtggcaccg gcgatgctat cccttccccc gcgtaggctc gaagagacca aacgcgcggg
     1321 acagagggag gacacgagat gagcgtgacc tggggacgaa tcctggtcgg tatcgcagtc
     1381 gccatgtgtc tcctcggcgt gggcgttctc gaggtgctga gcgagcgggg cgagagtcgc
     1441 gtgctcccgg aaccgtcgcg gatcggcgag gtgcggcccg agccatgggg cgaattcggc
     1501 cgcgtcgacc cggctctgac cgcggacgca gacgtgtcga cgccagccgc ctcgcagggc
     1561 atgaccgggg ccgtgcagca ccgacgcctg acgattctgg aggtcaacga gcgggccggg
     1621 cgcctgatct cgctgaccgg ggccggacgg gtcctcgtca ccgatctgag ccgggacccc
     1681 ttcgtggtca ccgaggacag cggggcgggc tcgctggctc tcttgaagcc gggggacgtg
     1741 gtgcggatcg agccgatgag cggacggact cagcgcatcg tcgtgctgcg tcacggctgg
     1801 caggagctgg agagcccgga gaagtgaagc gagctactta gggttgacgc acctgatgag
     1861 gcgccagccg ttgaagaggt tgaccttcgc ggtgatgtcc ggaacgaggc cgacctgctt
     1921 caagagcggc tcgagcggga gattcgactt ccagccgagc cgcgtgcaca ccggagcgac
     1981 gaggtcctcg aggcgcccga ccgcggggcg ctcgctccgg aagtggttga gcacgacgat
     2041 gttgccgccg ggcctcacca cccgcttcat ctcgcgcagc accccgaccg ggtcgggcac
     2101 cgcgctgatg gtgtaggtgg cgacggcgga ctcgaactca ccctgcccga agtccatggt
     2161 ggtggcatcc atggtcttga gcgtcacgtt ccccagggcc agctcgacga ccttgtcctg
     2221 ggctttctcc agcatctcct cggagaggtc gatgccggtg aggtgacagt gggaggggta
     2281 cagcgggagg ttcagcccgg tgccgatgcc cacctcgagg acgcggtccc cccgcttgat
     2341 gtcgagcagg cgaatggccg cctcccgacc ggggtggaag atccaatcga agacgaagtc
     2401 ataggcgggc gcgtacagct tgtacgccct cttgacctgc ctcttttcca gtgcggcgtc
     2461 caatccggca gccctccgat gtgaactgac acttcctgcc tccgcacttt tagcataggg
     2521 tgacgcagct tgccaagtgc tttatgaccc cttgatctgc aaagggaaag caaacttgcg
     2581 aagcgggcga cctacaggtc ggcttcctgg cagagctggc ccagaacctg gtctgacccg
     2641 aagtactcga gcgcgatccg ccgggcctcc cggctgtggc gctcgtagtc gcggttgacc
     2701 tcgtcgatcg cggctgcggc ttcctcgacg ccctcgaagg ccagcaggcc cacgcccgcc
     2761 gggatgtact taccgaagcc ggtgtcctgg gtcacgaccg gcttgccggc ggccagatag
     2821 cacacgctcc ggtcgctgaa ccagccactc cgtgtccgag cgaccaggtc cttcgacacc
     2881 gtgaactccc cgcgcgactg ctggatgtag cgctggtagg tggtcaggtc ccgggagacc
     2941 tggaaggcct cctggaggcc ccaaccgttg cgggccagga gatcgcgcat cgccgggtcc
     3001 tccacctcca gcgccatctc gagcgcctgc cgggtgcggc tcgggaggtc catgacccgg
     3061 aggaagttga ggtgcttgga ccagagatac gtttctcccc ggaagtggat gtccttgccc
     3121 gcgttcttcc aggtggcgat cgtggtgaag cgctccgctg ccgggtcggt gggggtgctc
     3181 cagaggtccg ggatcaccgg agggcgggtc gggcgccacg cgaacttctc gagcgggatg
     3241 gggctatcgg gctgccccag gttctccccg tacgtgaagt gatgggtgtg atcctcgagc
     3301 gccaggatgg cctcccggtc gccctccgcc acccggatct gctcgaacac cgggtcggtc
     3361 tccacgtaca cccggatcgg gcagcgcagg tgctcctccg tcagccgggt ggcgccgcag
     3421 acgttgaaga gagcgtcggc gtcccggaag aggtcggaga gccgctcgcg cgacagcccg
     3481 tgatgtgcag aggtcaccgc gtccacgtag ctccagcggt cggccagccc gaagcgcccc
     3541 atcacttccg cgaggaaggc cacgctgtag gcggcgttgc cgaccacgct cttgacccgt
     3601 gggtcgtacg ggtgagcccc cgagtcctcc acgtagtaga cgtcgtgtcc gaggcgggtc
     3661 aatcccagca cgtagtggat ggcctgccag ccgatgccgc ccagggggta gcgcccggct
     3721 aggtgcagga ccacgatgcg cttacgggat ggcaagcggg tccgcctgcc gatcggtggc
     3781 gcgcgccccg tacatcgcgg cgaacggccc tggccgcgcg agcagcgtct cgagcgagcc
     3841 ctgctccgcc acctcgccgt ccttgatcac caccaccacg tcggccattc ggaccgtcga
     3901 gagacggtgg gcgatgatga aggtggtgcg cccgacctgc agctgctcga gggcctcgat
     3961 gatcagccgc tcggtctccg gatcaagcgc ggaggtcggc tcgtccagga tgaggatggg
     4021 cgagtcgcgc aggatcgccc gcgcgatggt caggcgctgc ttctcgccct ccgagagggt
     4081 gacgccgtgc tcgcccacga ccgtgtcgta gccctggggc aggcgcgcca ccatgtcgtg
     4141 gatgcgggcg gccttcgccg cgtccacgat ctcctccagg cgggccccgg gccggccgaa
     4201 ggcgatgttc tcgcggatgc tcaccgggaa gagcagcggc ggctgcagga ccatcgcgac
     4261 ctggcggcgc agcgactgga gccggtactc gcgcgcgtcc accccgtcga cgagcacgcg
     4321 cccgcgggtg ggatcgtaga agcgcggcag caggctcagg agcgtcgact tgccggcccc
     4381 ggtcgggccg accaccgcca cgcgctgacc gggggtgacg tggagatcga cgccgcgcag
     4441 gatggggacg cccggagcgt actggaagtc caccccttcc cagatcacct cgccgcgcgc
     4501 accctcgggc gggaagctcc ggctgccgtc ggccacgtcc cgctccacgt ccatcgtgtc
     4561 gaggacgcgc tgcacgccca ctcgcgcgct ctgcgccagc ccgtagacct ggaacatgtt
     4621 gttgatgggg ccgtacagcg aggccaggta ggagatgaac accatcaggc tgccgacggt
     4681 cagggagccg tcgagcacgt ggcgggcgcc gacccacacc accgccgcgg tgccgagggc
     4741 gatcacgagg ttgaccacgc ccgagtagaa ggtctggagc gtgtagaggc ggagccccgc
     4801 gtccaggctg cgctcgctcg cgctcatgaa gcgccggtgc tcctcgtcct cgcgggtgaa
     4861 ggcctggatg acccgcatcg cctgcatcgc ccgctggacg acgccgtaga cctcgctctc
     4921 ccgctcgcgc atctcgcccg ccgcgacggt gatgcgccgg ttgagccgac cgatggcgag
     4981 caggagcagc gggcacacgc ccagggccag gaaggtgagc tcccggtcga gatggaacat
     5041 gatcgcggcc atcccgccca gcaggatgag cgcggtggtg gcggggaaca ggcagttcat
     5101 ggtcagcgcc tgcagcgcga aggtgtccgc ggtgacccgg tagagaaggt ccccggcctg
     5161 ggcccggctg tggaaggcca tggagagccg atgcaggtgc gcgtagaggt cgctgcgcag
     5221 gtcgcgcacc atccgctgcc cgatgcggat ggtggtgtag ttggtgagca cggtcaggct
     5281 gccgagggca gcatagatca atacgagcgc gatgcaggcg gtcaggagca gggccccggg
     5341 gctccagccc ccgccgaggc cgcccggcgt aggctggccg ccgaggaccg agtcgatgat
     5401 gaacttgatc ggccagggct tgagcagctc gagcgcggtc gtcaggcaca cctgggccag
     5461 cgcccacagg atgcgcccgc ggtgcggcca gaggtacccg gagacccgcc cggcgaagct
     5521 gctcacttca cctcgaccgc gctggcgtcg agcggcgggg catagtgtag ccgcgcccgc
     5581 cgggccacgc cgtggagcgc gtcgtagagc atgcgcccca gcgccaccga gtcgcggagt
     5641 atcgcgacgg cggccacgac tcccgcggcc
//
LOCUS       DATMCV010000565         5162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_898152, whole genome shotgun sequence.
ACCESSION   DATMCV010000565 DATMCV010000000
VERSION     DATMCV010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898152"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /gene="murJ"
                     /locus_tag="VNU03_12990"
     CDS             complement(<1..1221)
                     /gene="murJ"
                     /locus_tag="VNU03_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWP75430.1"
                     /translation="MSDSPEGHEQVQRGVVSAVGAIGLATLASRVLGYVRDMVVAHTF
                     GAGPVTDAFLVAFRIPNLLRRLLGEGALSTAVIPVFTETLTRGGPTAFGRLARAATGA
                     AIVVLCVVSALGILLSHQVVAIMAPGWRADTGLFDLAVTLTRVMFPYLVLVGLAALAM
                     GALNAHHRFFTAAIGPAVLNVAMIVAVLGLASRMNPPVMALAVGVLVGGLGQLLVQLP
                     ELRRLGVPLTPSAEWRHPAVARIAHRLWPAVFALAAVQITVVVNTLLASLLPTGTVSY
                     LYYADRVMEFPLGIFGIALATAALPSMSAQAARGELAGLRATLEFALRMAAFVAIPAA
                     VGLVLLGGPIVRLLFQRGEFSAADAVLTAQALTGYAVGLPAFSATRIAAQTFYALGDT
                     RTPVIVGFLSVAVNV"
     gene            1300..1560
                     /gene="rpsT"
                     /locus_tag="VNU03_12995"
     CDS             1300..1560
                     /gene="rpsT"
                     /locus_tag="VNU03_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWP75431.1"
                     /translation="MANTRSARKRIKQNEKRRLRNRAVRSAVRSSVKGAREALAGKSA
                     ESATAVHDAIRAIDRAVTQGIIHRNTAARRKSALARKLNAPA"
     gene            complement(1544..2899)
                     /locus_tag="VNU03_13000"
     CDS             complement(1544..2899)
                     /locus_tag="VNU03_13000"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HWP75432.1"
                     /translation="MRGLMSRALGALAAAVVLLVSGVPLAAAAPPARLLAGAVSVTVS
                     LPDDTPLGGYGGFPRRAWLPDLIGRYPDTFWFRPTTGEHDPIRVRALLLEAGSVRVLW
                     LALDLVGMDPTLLADLRARLDQLGLRYAAVIAAASHTHSGPGAYARSDLFGLLALDRE
                     SPRVRGRIFAAMEAAAREAERRKRPATVGTGRVEVADITQSRVHGPLDPELGVLKVVG
                     ADKRPIAVVWNYAIHGTMLGRENLKLSGDVMGDAAARIEEQLGAPALYVNGAVGDVSP
                     RQRGWDGVAATGKVLSAAVLALWPRIAADADQRVITAAAVAALPAPGLELRNCLGRWI
                     PRGTRLGLRSALPSTAEIAAVSVGRGAWVAIPGELDTQLGLEIKAAGRARFAHTFVAG
                     VANGYQGYFIAAQHFRAPSYISCGSLYGERGGEIVRDAALGALRDVAERRAPRPVKPA
                     R"
     gene            complement(2887..3882)
                     /gene="holA"
                     /locus_tag="VNU03_13005"
     CDS             complement(2887..3882)
                     /gene="holA"
                     /locus_tag="VNU03_13005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWP75433.1"
                     /translation="MDYAGFVKAADAGQAPPVTLLHGPEPFLLEDALARLTRALFPGQ
                     TELSLVREVFDARAAGAEAVVQSALMLPWIGERRLVIARGVDGLAAKAGESLAAYAKA
                     PNPSTALVLVADALLEGGHWLVRAVPASAVVAAPALGGRALTGWLRGHAQAQGYELRE
                     DAANLLVELSGDDLTQLVGEVEKAALAGGPDNRRVGTAEVRAVVGEHRLRHVFDLTRA
                     LSARDTGTALALLESLLNAGEEPLAVLGMLAREARATWQAAGALRAGRPAEDIARALR
                     RPPAAAAALLERARSVSPAAAARQVARCWDVERRLKLGGAARPELSLLVADLCAV"
     gene            complement(3883..4383)
                     /locus_tag="VNU03_13010"
     CDS             complement(3883..4383)
                     /locus_tag="VNU03_13010"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HWP75434.1"
                     /translation="MGRALGLILAAVVLAGCGYSTKGSLPDHIKTVAVPIFKNRTLEP
                     GVDSAITSGVVNAFSSGGRVKVVPIDEADAILQGEVVAYSLEGLAFDRNANVQAYRLR
                     LVLNVEFRDVKRSAMLWRQEGLSETSDFQVQGQVSDTIARARGAVSQAAVEIGRKVVN
                     LALDRF"
     gene            complement(4397..>5162)
                     /locus_tag="VNU03_13015"
     CDS             complement(4397..>5162)
                     /locus_tag="VNU03_13015"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWP75435.1"
                     /translation="KDGAKMSKSKGNVVDPDELIRRFGADTARLFSLFAAPPEKDLDW
                     NDRGVEGASRFLNRVWRFVHANLPELKTAPRRPSGAGSDAGRAFRRVIHETIRKVTHD
                     IEHDFHFNTAISAVMELVNALHEFERASLDGMPREERSALLREAVEATVLLLGPVSPH
                     ITEELWAALGHTQSLFKQRWPEPDPAALARDEVEIVVQVDGRVRGRLTALVGAQEADV
                     REQALADDKVRPWLDGRRIAKVVVVPGRLVNIVTRG"
BASE COUNT          752 a         1951 c         1884 g          575 t
ORIGIN      
        1 cacgttgacc gccaccgaga ggaaccccac gatcaccggc gtgcgcgtat cgccgagcgc
       61 gtagaaggtc tgcgccgcga tccgggtcgc cgagaacgcg ggcagcccca ccgcgtagcc
      121 ggtcagcgcc tgggcggtga ggaccgcgtc ggccgcgctg aactcgccgc gctggaagag
      181 caggcgcacg atgggcccgc ccaggagcac gagccccacc gcggccggga tcgccacgaa
      241 ggcggccatc cgcagcgcga actcgagggt cgcgcgcaga cccgcgagct cgccccgcgc
      301 ggcctgggcc gacatgctgg gcagcgccgc ggtggcgagc gcgatgccga agatgccgag
      361 cgggaactcc atcacgcggt cggcatagta gagatacgag acggtgccgg tcggcaacag
      421 cgaggccagc agggtgttca ccaccaccgt gatctgcacc gccgcgagcg cgaacaccgc
      481 gggccagagg cggtgggcga tccgggccac cgcggggtgg cgccactcgg cggagggagt
      541 caacggcacc ccgagccgcc gcagctcggg gagctggacc aggagctgcc cgaggccgcc
      601 caccagcacg cccaccgcca gggccatgac cggcgggttc atgcgtgagg cgagccccag
      661 caccgccacg atcatggcca cgttgaggac cgcggggccg atggcggcgg tgaagaagcg
      721 gtgatgggcg ttgagcgcgc ccatcgccag cgcggccagc cccaccagca ccagatacgg
      781 gaacatcacg cgagtcagcg taaccgccag gtcgaacagc ccggtgtcgg cgcgccagcc
      841 gggcgccatg atcgcgacca cctggtgcga gagcaggatt ccgagggccg agaccacgca
      901 gagcaccacg atggccgcgc cggtcgcggc gcgcgccagg cggccgaagg cggtggggcc
      961 gccccgggtc agggtctcgg tgaacaccgg gatcaccgcg gtggagagcg cgccctcccc
     1021 caggagccgc cgcagcaggt tcgggatgcg gaaggccacc aggaaggcgt cggtcaccgg
     1081 ccccgcgccg aaggtgtggg cgaccaccat gtcccgcacg tagccgagca cccggctcgc
     1141 cagcgtggcc aggccgatcg cgcccaccgc gctgacgacc ccgcgctgga cctgctcgtg
     1201 accctcgggg gagtcgctca ccggggggac atcgtccttg acacccggag gcccggacgg
     1261 taggctcgtg tgtcgctcac gtttgaaagg agatacaccg tggccaacac ccgctccgcg
     1321 aggaagcgga tcaagcagaa cgagaagcgc cggctgcgca accgcgcggt gcggagcgcg
     1381 gtgcgctcga gcgtcaaggg cgcccgcgag gccctcgccg gcaagagcgc cgaatccgcc
     1441 accgcggtgc acgacgccat ccgcgcgatc gaccgcgcgg tgacccaggg gatcatccac
     1501 cgcaacaccg ccgcccgccg caagtccgcg ctcgcccgca agctcaacgc gccggcttga
     1561 ccgggcgcgg cgcgcgccgc tcggcgacgt cgcggagcgc gccgagcgcg gcgtcccgga
     1621 cgatctcccc gccccgctcc ccgtagagac tgccgcacga gatgtagctc ggcgcccgga
     1681 agtgctgggc ggcgatgaag tatccctggt agccgttggc gacccccgcc acgaaggtgt
     1741 gcgcgaatcg cgcgcggccc gcggccttga tctcgagccc gagctgcgtg tcgagctcgc
     1801 ccgggatggc cacccaggcc ccgcgcccga cgctcaccgc cgcgatctcg gcggtggacg
     1861 gcaacgcgga gcggagcccg aggcgtgtgc cgcgcggaat ccaccgcccg aggcagttgc
     1921 gcagctcgag cccgggcgcg ggcagggccg cgaccgcggc cgcggtgatc acccgctgat
     1981 cggcgtcggc cgcgatgcgc ggccagagcg cgagcaccgc ggcggacagg accttgccgg
     2041 tcgcggccac cccgtcccag ccgcgctgcc gggggctcac gtccccgacc gcgccgttga
     2101 cgtagagcgc gggagctccg agctgctcct cgatccgcgc ggccgcgtcc cccatcacgt
     2161 cacccgagag cttgaggttc tcccgcccga gcatcgtccc gtggatggcg tagttccaca
     2221 ccaccgcgat cggccgtttg tccgcgccca cgaccttgag cacgcccagc tccggatcga
     2281 ggggcccgtg cacgcggctc tgcgtgatgt ccgcgacctc cacgcgcccg gttccgacgg
     2341 tcgccggccg cttgcggcgc tcggcctcgc gggccgccgc ctccatggcg gcgaagatgc
     2401 gcccccgcac ccgcggcgac tcccggtcga gagcgaggag cccgaagaga tcggagcgcg
     2461 cgtaggcccc cgggccggag tgggtgtgcg aggcggccgc gatcaccgcc gcgtaacgga
     2521 ggccgagctg gtcgagccgc gcccggagat ccgccagcag cgtgggatcc atgccgacca
     2581 gatcgagcgc gagccacagc acccggacgc tccccgcctc gaggagaagc gcgcgcaccc
     2641 tgatcgggtc gtgctcgccg gtggtgggcc ggaaccagaa ggtgtccggg tatcggccga
     2701 tcaggtccgg cagccaggcg cgccgcggga agccgccgta gccgccgagc ggcgtgtcgt
     2761 cgggcaggct caccgtgacg ctcacggccc cggcgagcag gcgcgcgggc ggggccgccg
     2821 ccgcgagggg aacacccgac accaggagga cgaccgcggc cgcgagcgcg cccagggcgc
     2881 gcgacatcag accgcgcaca gatcggcgac gagcagggac agctcggggc gcgcggcgcc
     2941 gcccagcttg aggcgccgct ccacgtccca gcagcgggcc acctgccggg ccgccgcggc
     3001 gggcgacaca ctccgcgcgc gctccagcag ggccgccgcc gcggcggggg gacggcgcag
     3061 ggcgcgcgcg atgtcctcgg ccgggcgccc cgcccgcagc gcgcccgcgg cctgccacgt
     3121 ggcgcgcgcc tcgcgggcca gcatgcccag caccgcgagc ggctcctcgc ccgcgttgag
     3181 gagcgactcg agcagcgcca gggcggtccc ggtatcgcgc gcggagagcg cgcgggtcag
     3241 gtcgaacacg tggcggagcc ggtgctcgcc caccaccgcc cggacctcgg cggtgccgac
     3301 gcggcggttg tcgggtcccc ccgccagcgc cgccttctcg acctcgccga cgagctgggt
     3361 gagatcgtcg cccgacagct cgaccagcag gttggccgcg tcctcccgga gctcgtaccc
     3421 ctgcgcctgg gcatggccgc ggagccagcc ggtcagcgcg cgcccgccga gggccggcgc
     3481 cgccaccacc gcggacgcgg gcaccgcccg gaccagccag tgcccgccct cgaggagggc
     3541 gtcggcgacc aggaccagcg cggtggacgg gttcggcgcc ttcgcgtagg cggccagcga
     3601 ttcgccggcc ttggccgcga ggccatccac cccgcgcgcg atcacgagcc gccgctcgcc
     3661 gatccacggc agcatgaggg ccgactgcac caccgcctcc gcgcccgccg cgcgggcgtc
     3721 gaagacctcg cgcaccagcg acagctcggt ctgcccgggg aagagcgcgc gggtcagccg
     3781 ggcgagggca tcctcgagga ggaagggctc ggggccgtgc aggagcgtga ccggcggcgc
     3841 ctgtccggcg tcggccgcct tgacgaaccc cgcgtagtcc acctagaagc gatccagcgc
     3901 caggttgacc accttccgcc cgatctcgac ggcggcctgg ctcaccgcgc cgcgcgcccg
     3961 ggcgatcgtg tcggacacct gcccctggac ctggaagtcc gaggtctccg acagcccctc
     4021 ctgacgccag agcatcgcgc tccgcttcac gtcgcggaac tccacgttca gcaccagtcg
     4081 gagccggtag gcctggacgt tggcgttccg gtcgaaggcg aggccttcga ggctgtaggc
     4141 caccacctca ccctgcagga tggcgtcggc ctcatcgatc ggcaccacct tcacccgccc
     4201 tccgctcgag aacgcgttga ccaccccgga ggtgatcgcg ctatccaccc cgggctcgag
     4261 ggtgcggttc ttgaagatgg ggaccgcgac ggtcttgatg tggtcgggga ggctgccctt
     4321 ggtgctgtag ccgcacccgg cgaggacgac cgccgccagg atcaggccga gcgcgcgtcc
     4381 catccggcga cgacgcctag ccacgggtca cgatgttgac gaggcggccc ggcaccacca
     4441 cgaccttcgc gatgcggcgg ccgtcgagcc acgggcgcac cttgtcgtcg gccagggcct
     4501 gctcccggac gtcggcctcc tgggccccga cgagcgcggt gaggcggccg cgcacgcggc
     4561 cgtccacctg caccacgatc tccacctcgt cgcgggcgag cgcggcgggg tcgggctcgg
     4621 gccaccgctg cttgaacagg ctctgggtgt ggccgagggc cgcccacagc tcctcggtga
     4681 tgtgcggcga gaccggcccc agcaggagga cggtcgcctc gaccgcctcg cgcagcagcg
     4741 cggagcgctc ctcgcggggc atcccgtcga gggaggcgcg ctcgaactcg tggagcgcat
     4801 tgaccagctc catcaccgcg ctgatcgcgg tgttgaagtg gaagtcgtgc tcgatgtcgt
     4861 gggtcacctt ccggatcgtc tcgtggatca cccggcggaa ggcgcgcccc gcgtccgagc
     4921 ccgctccact cggccggcgc ggcgcggtct tcagctcggg caggttggcg tggacgaagc
     4981 gccacacccg gttcaggaaa cgagaggcgc cctccacccc gcggtcgttc cagtcgaggt
     5041 ccttctcggg gggcgccgcg aagagggaga agaggcgcgc ggtgtcggcc ccgaagcggc
     5101 ggatcagctc gtcgggatcg accacgttgc ccttcgactt cgacatcttc gccccgtcct
     5161 tg
//
LOCUS       DATMCV010000566         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_27990, whole genome shotgun sequence.
ACCESSION   DATMCV010000566 DATMCV010000000
VERSION     DATMCV010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27990"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="VNU03_13020"
     CDS             <1..1697
                     /locus_tag="VNU03_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWP75436.1"
                     /translation="PGLVWLARHGLLIVLGAFLAIRADLAETPNWIAARGEALMLAAL
                     ALALVGGSSHAAAISPGVVSAVAIDITHLLGTGLWVGALVPLALLLRLASREDDADSR
                     PYAVRAARRFSRAALITMLILTGSGVMNAIVQVESLAGLAGTTHGRLLLAKLAVLVPI
                     LVLAAVSRAHILPALHGSSDTVRSSVMRRLAVFVGLEAGLALVLLALAAAMTLTTPAR
                     HGDPVWPLPFRFSLDTLLDVPATRGRALLGGQLMLLGLVAVIASLLVRRRRASVLTGA
                     LALVAIGAGAGLPPLVVDAYPTAYRRPLVTYHASSIAAGMMTYQEHCATCHGARGAGN
                     GATGRPPADLRAPPASRRLAGELFWLVTHGIPARGMPEFGSSLGEAQHWNVINFIRAI
                     GAADGARGIGRQVELDRAWLVAPDFTISMGPLAPAALRDYRGQRMVLLVLYTLPGSRA
                     RMAELARSYNVLSIIGVEVIAVPTRASPEAIRELGSSPPVFFPVVTDGNADIVATYGM
                     FAPGAHAELLIDRQGYIRAIWREDMGGMPRAAAVQSQVEKLNEEKSPPPFPDDHVH"
     gene            1720..2121
                     /locus_tag="VNU03_13025"
     CDS             1720..2121
                     /locus_tag="VNU03_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HWP75437.1"
                     /translation="MPVWQVALVLNLTLAVGLGLGYAAWGRRVEGLDREFDAARAQVE
                     RLERERQACAAGARAGLQQWEARGVVRAVYPQLLVITHEEIRGLLPARTTGFRLAEPT
                     VRDPIRVGDAIRFSLRGTVADDAGLVAIAPW"
     gene            2199..2378
                     /locus_tag="VNU03_13030"
     CDS             2199..2378
                     /locus_tag="VNU03_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75438.1"
                     /translation="MHERLIAWGLLVAVVMLTGCSSTAIPPTYTQGEVKAICERHNGW
                     WHPNDLIEGYCEAFP"
BASE COUNT          392 a          971 c         1021 g          484 t
ORIGIN      
        1 ggcccgggct cgtctggctc gcgcgccacg ggctcctgat cgtgctcggg gcctttctcg
       61 cgatccgcgc ggacctcgcg gagacaccga actggatcgc tgcccgcggc gaggcgctca
      121 tgctcgccgc gctggcgcta gccctcgtgg gcggctccag tcacgccgcc gcgatttccc
      181 cgggcgtggt gtcggccgtg gcgatcgaca tcacgcatct tctggggacc ggcctctggg
      241 tcggcgccct cgtcccgctc gcactcttgc tacgcctggc cagccgcgaa gacgacgctg
      301 actcccgccc ctatgcggtc cgcgcggccc gccgcttctc ccgcgccgcg ctgatcacga
      361 tgctcatcct gacgggctcg ggagtgatga acgcgatcgt gcaggtggag agcctcgccg
      421 gtctggcggg gacgacgcac ggccgtctgc tcctcgccaa actcgctgtc ctcgtcccga
      481 tcctcgtcct cgccgccgtg agccgcgcgc acattctccc tgcgctgcat ggctcgagcg
      541 acaccgtccg gagttccgtg atgcgacgcc tcgccgtgtt cgtcgggctg gaagccggcc
      601 ttgcgctcgt tctgctcgcg ctggccgcgg cgatgacgct gacgacgccc gcccgccacg
      661 gggatccggt ctggccgtta ccgttccgat tctcgctcga caccctgctc gacgtgcccg
      721 cgacgagggg ccgcgctctg ctgggcggcc agctgatgct gctcggcctc gtcgcggtga
      781 tcgcgtcgct gctcgtccgc cgccggcgcg cgtcggtgct caccggcgcc ctcgcgctcg
      841 tcgccatcgg cgccggcgct ggtctgccgc cactcgtggt cgatgcctat cccaccgcgt
      901 accggcgccc gctcgtgacg tatcacgcca gctcgatcgc cgccgggatg atgacctacc
      961 aggagcactg cgcgacctgc cacggcgcga gaggcgccgg caacggagcg acgggccgcc
     1021 cccccgccga tcttcgcgcg cctccggcgt cgcgccgtct cgcgggcgag ctcttctggc
     1081 tagtcactca cggtatcccc gcacgcggca tgccggaatt tgggagcagc ctcggtgaag
     1141 cccagcactg gaacgtgatt aatttcatca gagccatcgg cgccgccgac ggcgcgcgag
     1201 ggatcggacg ccaggtggag ctcgaccgcg cctggctggt cgcgccggac ttcaccatct
     1261 ccatgggccc gctcgcgccg gcggctctgc gcgactaccg cggccagcgg atggtgctgc
     1321 tggttctcta caccctgcca ggatcccgcg cccggatggc ggagctggcg cggagctaca
     1381 acgtactctc gatcatcggg gtcgaagtca tcgccgtgcc gacgcgggcc tcgcccgagg
     1441 cgatccgcga gctcggctcg tcgccgcccg tcttctttcc ggtagtgacc gacggcaatg
     1501 ccgacatcgt cgcgacgtat ggcatgttcg cgccgggggc gcacgcggag ctactgatcg
     1561 accgccaggg ctacatccga gcgatctggc gagaggacat gggaggcatg ccgcgggccg
     1621 ccgcggtcca gagccaggtg gagaagctca acgaagaaaa gagcccgcct ccgttccccg
     1681 acgaccacgt tcattgatga aaatgggagg gtcctcgtga tgccggtctg gcaggtggcc
     1741 ttggtgctga acctcacgct tgccgtcggg ctcgggctgg gctatgcggc ctggggccgg
     1801 cgggtggaag ggctggaccg agagttcgac gctgcccggg cgcaggtgga gcgactcgag
     1861 cgcgagcggc aggcatgcgc cgctggcgcg cgcgccggcc tccagcagtg ggaggcccgc
     1921 ggcgtcgtgc gagcggtcta cccgcagctg ctggtgatca cgcacgaaga gattcgtggg
     1981 cttctgccag cgagaaccac gggcttccgc ttggcggagc caacggtccg tgacccgatc
     2041 cgggtgggcg acgcgatccg gttttcgctg cgaggtacgg ttgccgacga cgccgggctc
     2101 gtggcgatcg cgccgtggtg aggactccac gagttgcgct cgagatgagt tgcaaagaag
     2161 tacggcgctc gcgcggtggg gtcggggagg cgtagagcat gcatgagcga ttgatcgcgt
     2221 ggggccttct ggtggccgtg gtgatgttga ctggctgcag cagcacagcg atcccgccga
     2281 cctataccca gggtgaggtg aaggccatct gcgagcgcca caacggctgg tggcacccga
     2341 atgatctgat cgagggatac tgcgaagcct ttccttaagg ctggaacttc tggctgtcgg
     2401 gattctcctg ctgacacagg ggacacggca tactgcgccc tcccagtgtg atcggagccg
     2461 ggaacggtcg gactggtggc gagagcttat gctgggggag cgggaggggt caaggagcgg
     2521 gcaggcgctg ccgctggcgg ggtgaccgag gtgaccggac tttcagatta cctctcacta
     2581 cgcgtggcca gacacgggat agccaaaccc cggtcacctg ggtcacctcg gccaggggcg
     2641 tgcccggcgg cgctggtgcg gacggcgctt ctacgtgaat tatagaggtg ggtggcgctg
     2701 cagcagcgcg tcgagcgcgg tcgggtcaac tggcttggtg aagtgggcgt cgaagcccgc
     2761 cgcgctggag cgcaggcgat cttcgcgctg cccgtatccc gtcacggcga tgaggaccat
     2821 gtctcggcgg ttcgcggctc ggatgcgctg ggcgacctga tagccgtc
//
LOCUS       DATMCV010000567         2796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_432541, whole genome shotgun sequence.
ACCESSION   DATMCV010000567 DATMCV010000000
VERSION     DATMCV010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432541"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1644)
                     /locus_tag="VNU03_13035"
     CDS             complement(106..1644)
                     /locus_tag="VNU03_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75439.1"
                     /translation="MPTRRDLFKQAGAAAALAALHPVAARAQARKPALTIGYPSTPET
                     IDPHQFRSVLSGSIIGLMGEGLLTRDPQTMEIKPLLAESVKNLNPNTWEVKLRKGVKF
                     HNGEDFTADSVKFTIERAINSKLNTLAKLTWPPSFGQEIVIVDPYTVKIVTKVPDPMV
                     PSRLAAESMNIAPARGLAEYREKFVTDRFVGTGPFRFVEHVVGDRVVVEANPAYWGPK
                     AASPRIVIQVIPDSATRLAALQRGDVDVMLNLPIPLAPTVEADSSLRVYSELSSLTHG
                     ILLNARESAPLKDRRVRQALNMAVDRAAILKGLYSGRGQFLNTVTGKNVANTVDPGAY
                     PYDPARAKALLAEAGFGQGFEMTMWQSIGRWVLAEETAQVIAGYWDKIGVRTKVQVLE
                     WAEYNKRSAAGAHKDCFYYAFINGTWDASYTVQRFKPDFQTFRYFDASGDLLKTIEEY
                     ERTFDPRKRKELGAKALTGLHDEAVWLFLWQLDELFGVSKKVKGFKMRPDNVLWMRDA
                     YVEA"
     gene            1717..2481
                     /locus_tag="VNU03_13040"
     CDS             1717..2481
                     /locus_tag="VNU03_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75440.1"
                     /translation="MARLDEKVAVVTGAAHGLGRAIAERLAGEGATVVLGDVDAAALE
                     QTVARIVGAGGRAVGVVGDVTEEEPAARLIASAVERGGRLDILVNNVGGSRNARIWEM
                     AVADWDFTLRLNLRSTFLCTRAAVPHMMKRRYGRIVCISSGAREGTPWTAYYQGGSAY
                     SAAKAGVHGFIRDVALELAEHGINVNAVAPGPIDTERVGDALRRLNETVEYSPNRMTP
                     LRRLGRPEEVADAVLFLASDEATYITGHTLAVTGGR"
BASE COUNT          426 a          927 c         1016 g          427 t
ORIGIN      
        1 agcaccgggt cacccgacag gtgaaccagc ccgaagacca cgagcgagac cccgaccgcc
       61 acgacggcgg cgtggcggag ccggcgcagg aggaagggaa gcgcttcagg cctccacgta
      121 ggcgtcgcgc atccagagca cgttgtcggg gcgcatcttg aaccccttga ccttcttcga
      181 gacgccgaag agctcgtcga gctgccagag gaagagccac accgcttcgt cgtgcaggcc
      241 ggtgagcgcc ttggccccca gttccttgcg cttcctggga tcgaaggtgc gctcgtactc
      301 ctcgatggtc ttgagcagat cgccggaggc gtcgaagtag cggaaggtct ggaagtcggg
      361 cttgaagcgc tgcacggtgt aggacgcgtc ccacgttccg ttgatgaacg cgtagtagaa
      421 gcagtccttg tgggcgcccg cggcggagcg cttgttgtac tcggcccact ccagcacctg
      481 caccttggtc cggacgccga tcttgtccca gtagcccgcg atgacctgcg cggtctcctc
      541 ggcgagcacc cagcggccga tcgactgcca catcgtcatc tcgaagccct ggccgaagcc
      601 cgcctccgcg agcagggcct tggcccgcgc gggatcgtac ggatacgcgc cggggtcgac
      661 ggtgttggcc acgttcttcc cggtgaccgt gttcaggaac tggccgcgcc cggagtagag
      721 gcccttcagg atcgccgcgc ggtccaccgc catgttgagc gcctggcgca cccggcggtc
      781 cttcagcggg gccgactcgc gggcgttgag caggatgccg tgggtgagcg aggacagctc
      841 ggagtacacc cgcaggctcg agtcggcctc cacggtgggg gcgagcggga tggggaggtt
      901 cagcatcacg tccacgtcgc cgcgctggag cgcggccagc cgggtggccg agtccgggat
      961 gacctggatc acgatgcgcg gcgacgccgc cttcgggccc cagtaggcgg gattggcctc
     1021 gacgacgacc cggtcgccga ccacgtgctc gacgaagcgg aagggcccgg tgccgacgaa
     1081 gcggtcggtg acgaacttct cccggtactc ggccaggccc cgcgcgggcg cgatgttcat
     1141 ggactcggcg gcgagacggc tcggcaccat cgggtccggc accttggtga cgatcttcac
     1201 ggtgtaggga tcgacgatga cgatctcctg gccgaacgac gggggccagg tgagcttggc
     1261 cagggtgttg agcttcgagt tgatcgcgcg ctcgatggtg aacttcaccg agtccgcggt
     1321 gaagtcttcc ccgttgtgga acttgacccc cttgcgcagc ttcacttccc aggtgttcgg
     1381 gttgagattc ttcaccgact ccgcgagcag cggcttgatc tccatggtct ggggatcgcg
     1441 ggtgagcagg ccctcgccca tgaggccgat gatcgagccg ctcagcaccg agcggaactg
     1501 gtgcggatcg atcgtctccg gcgtcgacgg gtagccgatg gtgagcgcgg gcttccgggc
     1561 ctgggcgcgc gcggccaccg ggtggagggc ggcgagcgcg gcggcggctc cggcttgctt
     1621 gaacaggtcg cggcgggtcg gcatcgggtc gaatcctcct gggatcgaga ggtggcgggg
     1681 ggattttacc tcaggcggcg tggtaatgtg ccggccatgg cccgactgga cgagaaggtg
     1741 gcggtggtga cgggcgcggc ccacggactc ggccgcgcga tcgcggagcg cctggccggc
     1801 gagggcgcca ccgtggtgct gggcgacgtg gacgcggccg cgctcgagca aacggtcgcc
     1861 cggatcgtgg gcgccggcgg ccgggcggtc ggcgtggtgg gtgacgtcac cgaggaagag
     1921 ccggccgcgc gcctgatcgc ctccgcggtc gagcgcggcg ggcgtctcga catcctggtc
     1981 aacaacgtgg gcggcagccg caacgcgcgc atctgggaga tggcggtcgc ggactgggac
     2041 ttcaccctgc ggctcaacct gcgctcgacc tttctctgca cccgcgccgc ggtgccgcac
     2101 atgatgaagc ggcgctacgg ccgcatcgtc tgcatctcgt cgggcgcgcg cgagggcacc
     2161 ccgtggaccg cctactacca gggcggctcg gcctactcgg cggccaaggc cggcgtgcac
     2221 ggcttcatcc gcgacgtcgc gctcgagctg gccgagcacg ggatcaacgt caacgcggtg
     2281 gcgcccggtc cgatcgacac cgagcgggtg ggcgacgccc tgcggcggct caacgagact
     2341 gtggagtaca gtcccaatcg catgaccccg ctgcggcgcc tcggccgtcc ggaggaagtc
     2401 gccgacgcgg tcctgttcct ggcctccgac gaggccacct acatcaccgg acacaccctc
     2461 gccgttaccg gcggccgcta gggaggccgc cgataaaggc cccatctgcg ggagtgcggc
     2521 gccctcggcg gcacgctcga cgtacggaga gtacgcctcg cgtgccgcct tcgggcgccg
     2581 cctcgcagct gggcctttct cggcggcctc caaggaggaa taaccaatga ccaagagtga
     2641 gctgatgcag aaggtccagg cgacgatcga ccggcggggc gacgagatca tcgcgctggg
     2701 cgagacgatc cgccgccacc cggagctcgg cttcaaggag ttcaagaccg ccaagctggt
     2761 cgcccagacc ttcgagcgca tcgggctcac cccgcg
//
LOCUS       DATMCV010000568         6339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_432547, whole genome shotgun sequence.
ACCESSION   DATMCV010000568 DATMCV010000000
VERSION     DATMCV010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432547"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VNU03_13045"
     CDS             <1..982
                     /locus_tag="VNU03_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWP75441.1"
                     /translation="RDITRGDLEAMVEPLVAATLGPCRQALTDAALGTDQVDEVVLVG
                     GSTRMPLVQRRVAELFGRPAHSRLNPDEVVALGAAVQAQILAGGITDMLLLDVTPLSL
                     GIETLGGIVSVLIPRNTTIPTLAKEYFTTSVDGQTAVDLHVLQGERELAKDNRSLARF
                     DLAGVDPMPAGMPKIEVTFLIDANGILQAQARELRTGKAASIEVKPTYGLTETQVEQM
                     VEDSYTYAEDDVNARLLIETQTEADTVINHVERALRQAGGIATAEERARIEAAMALLR
                     TTRAGTDRDQIRERTIELNQATAPLADRMMEAAIKGAVASKRADQLLENS"
     gene            983..1306
                     /locus_tag="VNU03_13050"
     CDS             983..1306
                     /locus_tag="VNU03_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWP75442.1"
                     /translation="MAVHKVTFLPLNRTVEVDDAKYPLADHGRPGSLLDIALAHNIHL
                     EHNCGGSCACTTCHVVVREGEGNLSPMEQDEEDRLDTAEGLTLHSRLGCQAVVRGDVV
                     VEIPK"
     gene            1331..1531
                     /gene="iscX"
                     /locus_tag="VNU03_13055"
     CDS             1331..1531
                     /gene="iscX"
                     /locus_tag="VNU03_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HWP75443.1"
                     /translation="MIWTDAEDIALALMDAHPDVDPLTVRFTDLHQWVVALPEFRDDA
                     KNSNEGILEAIQMKWHEEYQDR"
     gene            complement(1589..1822)
                     /locus_tag="VNU03_13060"
     CDS             complement(1589..1822)
                     /locus_tag="VNU03_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75444.1"
                     /translation="MAAFLHLLKADSAAALAASVIESNLAEPDARVTVVLLDRAIAPS
                     LPAGVTVRRLADGELDYPGLLDLIFAHDHVITW"
     gene            complement(1822..2175)
                     /locus_tag="VNU03_13065"
     CDS             complement(1822..2175)
                     /locus_tag="VNU03_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWP75445.1"
                     /translation="MSQNQPTLVVISEDPRVSHRANEAIRIALGIVAGDNPVHVVLTG
                     PAVHLLDEDTDDLVDGDDIAKFRAALKKLGVTFHVEGALPTAPGWNADGHPVDSVSRA
                     QIAALVPRATRYLVF"
     gene            complement(2183..2470)
                     /locus_tag="VNU03_13070"
     CDS             complement(2183..2470)
                     /locus_tag="VNU03_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75446.1"
                     /translation="MVSTAPDHPNLETALGLSKAALDGGGDVYLYLIDDAVAAVEDER
                     LQALADRGLKLFVCAYGCQKRRRPLSDRATNCGLVVLTDVINGTDRFVSLN"
     gene            2537..3691
                     /locus_tag="VNU03_13075"
     CDS             2537..3691
                     /locus_tag="VNU03_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HWP75447.1"
                     /translation="MNRQVYLDHNASTPVHPEVVEAMLPYFSERFGNPSSVHGFGREA
                     REGLETAREQVAHFLRVGKEEVVFTSGGTESDNMAVKGVALARGRGHIITSRIEHHAV
                     LRAVETLETQGFAATYLDVDGYGMVDPDALRRAIRPDTILISLMHANSEIGTIQPARQ
                     LGAIARERGIPFHMDAVQTFGKVPIDLDAFNIDMLSFSSHKIYGPKGVAGLYIRKGTK
                     MVSLQHGGEHERRRRAGTENVAGIVGFGKAVEIRGRDMAEEGQRLTGLRDRLWEGLKV
                     RVPEVRLNGHPTERVPGTCNVCFRHVESESIVLGLDLKGIGVSAGSACTSGNVEPSYV
                     LVAMGVPVEWAMGAVRHSLGRSTTAQDIDYVLDSTEPLVAKLRSAMPVRA"
     gene            3715..4107
                     /locus_tag="VNU03_13080"
     CDS             3715..4107
                     /locus_tag="VNU03_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HWP75448.1"
                     /translation="MQYSPALIDHFRNPRNAGRMRDPDGVGEGEYKECMDLVRFHLRV
                     REGRVVEARFQAYGCGPTIAAASAATEVATACGLEELLELSAARVEAAVGGLPDARRH
                     AAEVAVLGLRAAARDALDRSRTRSEPNV"
     gene            4100..4774
                     /gene="cysE"
                     /locus_tag="VNU03_13085"
     CDS             4100..4774
                     /gene="cysE"
                     /locus_tag="VNU03_13085"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HWP75449.1"
                     /translation="MFDRMRQDVRTVKERDPAARSTLEVVLCYPGVHAIWSHRVAHAL
                     WRGGWRLSARFVSHVARFLTGIEIHPAAVLGPGLFIDHGMGVVIGETAEVGQNVSILQ
                     GVTLGGTSVKREKRHPTIGDNVVIGSGAKIIGAFTIGAGSRIGAGSVVVREVPENCVV
                     VGVPGRITFRNGERVTGAIDLNQADLPDPLARTLEQMLDRIHTLEAEIERLKKASDAT
                     AGARAD"
     gene            4798..4989
                     /locus_tag="VNU03_13090"
     CDS             4798..4989
                     /locus_tag="VNU03_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP75450.1"
                     /translation="MLPKDMLPKDTMDVKEAARYLAMDEKTVAAMAVERRIPALEHEG
                     KWLFSKKSIDKWRGRTGAQ"
     gene            5114..6319
                     /gene="dinB"
                     /locus_tag="VNU03_13095"
     CDS             5114..6319
                     /gene="dinB"
                     /locus_tag="VNU03_13095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWP75451.1"
                     /translation="MTRTILHVDMDAFYASVEQRDRPELRGRPVIVGADPRGRGVVSA
                     ASYEARRFGVHSAMPIGRAARLCPDGAFVPVDMDKYAAVSREVMAILAAFTPLLEPVS
                     IDEAFLDVTGTEGLSGDGPTVAATIKARIAATLRLTASVGVAANKFVAKVASDLRKPD
                     GLVVVRPGTEGDFLAPLPVSRLWGVGRVTAEGLEAMGVTTIGQLAAVPVAYLEARFGS
                     SGRGLHDLSRGLDDRAVEPFAPPKSMGAEETFGHDHRDVERLAATLRGQAERVARELR
                     EGGYATRCVTLKLRFADFSTLTRRHTDDPTQDGLVIYQRARALLDRIPLRQAVRLIGL
                     SASGLGPAEAGQLSLLGPDPTRRERLARAMDRVTARYGEDSLRPASVLRGRRRGRPPE
                     GPGSPLPST"
BASE COUNT          978 a         2135 c         2345 g          881 t
ORIGIN      
        1 tcgcgacatc acccgcggtg atctcgaggc gatggtggag ccgctggtgg cggccaccct
       61 cggtccgtgc cggcaggccc tgaccgacgc cgcgctgggg acggatcagg tggacgaggt
      121 ggtgctggtg ggcggctcca cgcggatgcc gctcgtgcag cggcgggtgg ccgagctctt
      181 cggccggccc gcgcacagcc ggctcaaccc ggacgaggtg gtggccctgg gggccgcggt
      241 gcaggcgcag atcctcgccg gcggcatcac cgacatgctg ctcctcgacg tgaccccgct
      301 ctcgctcggc atcgagaccc tgggcggcat cgtgagcgtg ctgatcccgc gcaacaccac
      361 gatcccgacc ctggccaagg agtacttcac gacgtcggtg gacgggcaga ccgcggtcga
      421 cctccacgtg ctgcagggcg agcgggagct ggccaaggac aaccgctcgc tcgcgcgctt
      481 cgacctggcc ggggtggacc cgatgccggc cggcatgccg aagatcgagg tcaccttcct
      541 gatcgacgcc aacgggatcc tccaggcgca ggcccgcgag ctacggaccg gcaaggcggc
      601 cagcatcgag gtcaagccca cctacgggct caccgagacc caggtggagc agatggtgga
      661 ggactcctac acctacgcgg aggacgacgt caacgcgcgg ctgctgatcg agacccagac
      721 cgaggccgac accgtgatca accacgtgga gcgcgcgctg cgccaggcgg gcggcatcgc
      781 caccgcggag gagcgggccc ggatcgaggc cgcgatggcc ctcctccgga ccacgcgggc
      841 gggcaccgac cgggaccaga tccgcgagcg cacgatcgag ctcaaccagg ccaccgcgcc
      901 gctcgcggat cgcatgatgg aggcggcgat caagggcgcg gtcgcctcca agcgggccga
      961 tcagctcctg gagaattcct agatggcggt gcacaaggtg acgttcctgc cgctcaaccg
     1021 gaccgtcgag gtcgacgacg ccaagtatcc gctcgccgat cacgggcgcc ccggctcgct
     1081 gctcgacatc gcgctcgccc acaacatcca cctggagcac aactgcggag gcagctgcgc
     1141 ctgcacgacc tgtcacgtgg tggtgcggga gggcgagggg aacctctcgc cgatggagca
     1201 ggacgaggag gaccgcctgg acaccgcgga gggcctcacc ctgcactcgc gtctcggctg
     1261 tcaggcggtg gtgcggggcg acgtggtggt ggagatcccc aagtgaggtc gtccagatga
     1321 cctggcccaa atgatctgga cggacgcgga ggacatcgcg ctcgccctga tggatgcgca
     1381 tccggacgtg gatccgctca cggtgcggtt caccgacctg caccagtggg tggtggccct
     1441 ccccgagttc cgcgacgacg ccaagaactc gaacgagggg atcctcgagg ccatccagat
     1501 gaaatggcac gaggaatacc aggaccgcta gcctctccct ctccccccag gggagagggc
     1561 agggtgaggg gccaaagggt gagggggttt accaggtgat gacgtggtcg tgagcgaaga
     1621 tcagatcgag caggcccggg tagtcgagct cgccgtcggc gagtcgacgc acggtcacgc
     1681 ccgcgggcag cgacggcgcg atcgcgcggt cgagcagtac caccgtgacg cgggcgtccg
     1741 gctcggccag gttcgactcg atgacggagg cggccagggc ggcggccgag tccgccttga
     1801 ggagatggag gaacgcggcc atcagaagac gagataccgc gtggcgcgag gcaccagcgc
     1861 ggcgatctgg gcccggctca ccgagtccac cggatggccg tccgcgttcc agccgggcgc
     1921 ggtcggcagc gcgccctcca cgtggaacgt gacccccagt ttcttgagcg cggcgcggaa
     1981 cttcgcgatg tcgtcgccgt cgaccaggtc gtcggtgtcc tcgtcgagca ggtgcaccgc
     2041 gggcccggtg agcacgacgt ggactgggtt gtcgccggcc acgatcccga gcgcgatgcg
     2101 tatcgcctcg ttggcccggt gcgagacgcg cgggtcctcg gagatgacca ccagggtcgg
     2161 ctggttctgg ctcacgcgac ggctagttca gcgagacgaa gcggtcggtc ccgttgatca
     2221 cgtcggtgag gaccaccagg ccgcagttgg tggcccggtc cgacaggggt cggcggcgct
     2281 tctggcagcc gtaggcgcac acgaagagct tcagcccgcg atcggcgagg gcctgcaggc
     2341 gctcgtcctc caccgcggcg accgcatcgt cgatcaggta gaggtacacg tctccgcccc
     2401 cgtcgagcgc cgccttcgag aggccgagcg cggtctcgag gttcgggtgg tcgggagcgg
     2461 tggaaaccat caggccgagc ttccgtctgg ccgaggccat gcgggcgcag cgtaacatcg
     2521 tgaaagggga gcgtcaatga accgacaggt ctatctcgat cacaatgcct cgaccccggt
     2581 gcatccggag gtcgtcgagg cgatgctgcc gtacttcagc gagcggttcg gcaatccgtc
     2641 gagcgtgcac ggcttcggcc gcgaggcgcg cgagggcctc gagaccgcgc gcgagcaggt
     2701 cgcccacttc ctgcgggtcg gcaaggagga ggtcgtcttc acctccggcg gcaccgagtc
     2761 cgacaacatg gcggtcaagg gcgtggcgct ggcccggggc cggggccaca tcatcacctc
     2821 ccggatcgag caccacgcgg tcctgcgcgc ggtggagacc ctggagacgc aggggttcgc
     2881 ggccacctat ctcgacgtgg acggctacgg catggtggac ccggacgcgc tgcggcgggc
     2941 gatccgtccc gatacgatcc tgatctcgct catgcacgcc aactccgaga tcggcaccat
     3001 ccagcccgcc cggcagctcg gggcgatcgc ccgcgagcgc ggcatcccct tccacatgga
     3061 cgcggtgcag accttcggca aggtcccgat cgacctcgac gcgttcaaca tcgacatgct
     3121 gtcgttctcg agccacaaga tctacgggcc gaagggcgtg gccgggctct acatccggaa
     3181 aggcaccaag atggtctccc tccagcacgg cggtgagcac gagcggcggc gccgggccgg
     3241 gaccgagaac gtggccggca tcgtcggctt cggcaaggcg gtcgagatcc gcggccgtga
     3301 catggccgag gagggccagc ggctgaccgg gctgcgcgac cggctctggg agggtctgaa
     3361 ggtccgggta cccgaggtgc ggctcaacgg ccatcccacc gagcgggtgc ccggcacctg
     3421 caacgtgtgc ttccggcacg tggagtcgga atccatcgtg ctcggcctgg atctgaaggg
     3481 catcggcgtg tcggccggct cggcgtgtac gtccggcaac gtggagccat cgtacgtgct
     3541 ggtggcaatg ggcgtgccgg tggagtgggc gatgggcgcg gtgcggcatt cgctcggacg
     3601 gagcaccacc gcccaggaca tcgactacgt gctcgactcg acggagccgc tggtggccaa
     3661 gctgcgtagc gcgatgccgg tccgggcctg aggacggccg agccgggcgc gccggtgcag
     3721 tacagcccgg ccctcatcga ccacttccgg aacccgcgga acgcggggcg gatgcgggat
     3781 cccgatgggg tcggggaggg ggagtacaag gagtgcatgg acctggtgcg gttccacctg
     3841 agggtccggg agggccgggt ggtggaggcg cggttccagg cctacggctg cgggccgacg
     3901 atcgcggcgg ccagcgcggc gaccgaggtg gcgacggcgt gcgggctcga ggagctgctc
     3961 gagctgtcgg cggcgcgggt cgaggcagcg gtgggcgggc tgccggacgc gcggcgacac
     4021 gccgcggagg tcgcggtgct ggggctgcgg gcggccgcgc gtgacgcgct cgatcgaagc
     4081 cggacaagga gcgagccgaa tgtttgatcg gatgcggcag gacgtgagaa cggtgaagga
     4141 gcgcgacccg gcggcccgct cgaccctcga ggtcgtgctg tgctacccgg gcgtgcacgc
     4201 gatctggagc caccgcgtgg cccacgcgct ctggcgcggc gggtggcggc tgagcgcccg
     4261 gttcgtgtcc cacgtggccc gcttcctgac cggtatcgag atccaccccg ccgcggtgct
     4321 ggggcccggg ctcttcatcg atcacggcat gggggtggtc atcggcgaga ccgccgaggt
     4381 cggtcagaac gtgagcatcc tgcagggggt caccctcggc ggcacgagcg tcaagcgcga
     4441 gaagcgccac cccaccatcg gcgacaacgt ggtgatcggc tcgggggcca agatcatcgg
     4501 cgcgttcacc atcggggcgg ggagccggat cggggccggc tcggtggtgg tgcgcgaggt
     4561 gccggagaac tgtgttgtgg tcggggtgcc gggtcgcata acattccgca acggtgagcg
     4621 ggtgaccggg gccatcgacc tcaaccaggc ggatctgccg gatcccctgg cgcggaccct
     4681 ggagcagatg ctcgaccgga tccacaccct ggaagcggag atcgagcggc tcaagaaggc
     4741 gtccgacgcg acggcgggcg ctcgcgcaga ctgatccagg acaggaggcg atcgccggtg
     4801 ctgccaaagg acatgctgcc caaggacacg atggacgtga aggaagcggc ccgctatctg
     4861 gccatggacg agaagacggt ggccgccatg gccgtcgagc gccgtatccc cgcgctggag
     4921 cacgagggga agtggctctt ctcgaagaag tcgatcgaca agtggcgggg ccgcacgggc
     4981 gcacagtagc gggcataggc gaccgtttag ttcttcaagc tcttcatctg cgggggccta
     5041 gaaatggccc ccgcactccc ccagtcggcg gacaaccgtt cacaaccagt tggcggacac
     5101 cgtttacagc accgtgacgc ggacgatcct gcacgtcgac atggacgcct tctacgcgtc
     5161 cgtggagcag cgcgatcggc cggagctgcg cgggcggccc gtgatcgtgg gggcggatcc
     5221 gcggggacgc ggcgtggtct cggccgcatc ctacgaggcg cggcggttcg gcgtgcactc
     5281 ggcgatgccg atcgggcgcg cggcgcggct ctgccccgac ggggcgttcg tcccggtcga
     5341 catggacaag tacgcggccg tctcccggga ggtcatggcc atcctggccg cgttcacccc
     5401 cctgctggag ccggtctcca tcgacgaggc cttcctcgac gtcaccggca ccgaagggct
     5461 ctcgggcgac ggccccaccg tcgccgcgac gatcaaggcg cgcatcgcgg cgaccctccg
     5521 cctgaccgcg tcggtcggcg tggcggccaa caagttcgtg gccaaggtgg cgtccgacct
     5581 ccggaagccc gacgggctcg tcgtggtccg gccgggtacc gagggcgact tcctcgcgcc
     5641 gcttccggtc tcgcgcctct ggggcgtcgg ccgggtcacc gccgagggcc tcgaggcgat
     5701 gggggtcacc accatcgggc agctcgcggc ggtcccggtc gcatatctcg aggcgcgctt
     5761 cggatcgagc gggcgcgggc tccacgatct ctcgcgcggc ctcgatgacc gcgcggtcga
     5821 gccgttcgcc ccgcccaagt cgatgggcgc ggaggagacc ttcggtcacg accatcgcga
     5881 cgtcgagcgc ctcgccgcca cgctgcgcgg ccaggccgag cgggtggcgc gcgagctgcg
     5941 ggaagggggc tacgcgaccc gctgcgtgac cctgaagctc cgcttcgcgg acttctccac
     6001 gctcacccgt cgccataccg acgacccgac ccaggacggt ctcgtgatct accagcgcgc
     6061 ccgggccctg ctggatcgca tcccgcttcg ccaggcggtg cgcctcatcg gcctctcggc
     6121 atccgggctc ggccccgcgg aggcgggcca gctctcgctc ctcggccccg acccgacgcg
     6181 ccgcgagcgg ctggcgcgcg cgatggatcg ggtgaccgct cgctacggcg aagactccct
     6241 gcgcccggcc agcgtgctgc gcggccgacg tcgcggccgc ccgcccgagg gcccgggctc
     6301 cccgttgcct tcgacatagg cttcggctag actcgctga
//
LOCUS       DATMCV010000569         6717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_693172, whole genome shotgun sequence.
ACCESSION   DATMCV010000569 DATMCV010000000
VERSION     DATMCV010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693172"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VNU03_13100"
     CDS             <1..492
                     /locus_tag="VNU03_13100"
                     /inference="COORDINATES: protein motif:HMM:NF019940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HWP75452.1"
                     /translation="WPLLEKALGGALALMVERRETEGAALAQELGGLREALAGHVETV
                     ARRAPAAVERRTARLRERMQTMLGDTQIDPARLATEVAVWADRTDVSEELARLRAHLA
                     QLAALLAGDEPVGRTLDFLIQEINREVNTVGSKADDLEISQTVIAAKATLEKMREQVQ
                     NIE"
     gene            512..1123
                     /gene="gmk"
                     /locus_tag="VNU03_13105"
     CDS             512..1123
                     /gene="gmk"
                     /locus_tag="VNU03_13105"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWP75453.1"
                     /translation="MKRRGTLVVVSAPSGAGKTTLCHEVRTLVPDLYYSVSYTTRSPR
                     HDETNGNDFFFVTEGAFTAMRARDEFAEWAEVHGHLYGTPAKALESALGRGLDVLLDI
                     DTHGARQLRQRYPEAVSVFIMAPSMAELEARLRERNSNSPGDIARRLSRAKEEIAAWR
                     QYDYLIINRDVKDAVDQLATIIQAERCRTSRLTLRFPDMEVPA"
     gene            1123..1335
                     /gene="rpoZ"
                     /locus_tag="VNU03_13110"
     CDS             1123..1335
                     /gene="rpoZ"
                     /locus_tag="VNU03_13110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWP75454.1"
                     /translation="MSFPSLEKSLDNVSNRYMLVVLAAKRARQLNRGAKAQVDTRHKK
                     PTSTALEEIADARIGYRVKEEDTPKL"
     gene            1497..2732
                     /gene="coaBC"
                     /locus_tag="VNU03_13115"
     CDS             1497..2732
                     /gene="coaBC"
                     /locus_tag="VNU03_13115"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWP75455.1"
                     /translation="MSLNDKDLILGVTGSIAAFKAVYLLRELTRLGARVTVVTTAHAD
                     KFIGPLTFRTLSGRPVLSDLFDPQSPEAVEHVALAERAHALVVAPATANLLARAAHGI
                     ADDFLTTLLLAARCPVLMAPAMDGGMWDHAAVTANVRTLRERGVTVLEPDAGALASGL
                     SGRGRLPEVDAIVEALERVLAPRRDLAGERLLVTSGPTREPIDPVRYISNRSSGKMGQ
                     AVATAALRRGAEVTLISGPTALTPPPGAVYVPVQTAEDMREAALQHLERATIVIKAAA
                     VGDYRVKRPSATKIKSKKDEGLALDLVANPDILRELAARKGRAFLVGFAAETNDVRAN
                     AAAKLEAKGVDLLVANDVSVQGIGFEADDNQVTLLDRWGGVADLPRMSKLEVADAILD
                     RVLGLRSAQRAGTPDRTAR"
     gene            2737..3444
                     /locus_tag="VNU03_13120"
     CDS             2737..3444
                     /locus_tag="VNU03_13120"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWP75456.1"
                     /translation="MSDPREAVALALRGLADTLRHHRDLGLRDLTLASVPFPDPADAL
                     REQERARQGCQLCKLCKSRTNVAFGSGHARARLMVVGEGPGEEEDKQGLPFVGRAGQL
                     LTKMLASVGFDRERDCYIANVVKCRPERNRNPEPDEVAACSPFLMAQIDTVRPHVILA
                     LGNFAAQTLIGTKEGITRLRGKTYAFRTAVLVPTFHPAFLLRNPGQEFKRMAWDDLKL
                     ARREYDARATDGGRTDG"
     gene            3441..5207
                     /locus_tag="VNU03_13125"
     CDS             3441..5207
                     /locus_tag="VNU03_13125"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75457.1"
                     /translation="MIADIVFDIPIDHAFSYAVPAGLDVTRGQRVSAPLHGRSRVGVV
                     VGLREGDATALKPLQRAVEPVPVLSDAALELTRWAAGESLSSWGSTLLSLLPPAPRPS
                     AAETVSPPADPPPSPAHEPELWVDHRREARLAEHLERETGAALVITPDREAAARWARR
                     LDAVRLDSGVPEPERRRAWFAASRGRSRVVVGTRSALLAPLPPPATLALIDEHDPAHK
                     PPGAPRLHSRDLLLRRAALDGSRLLLLSATPSVETWWRAQDGQIARAEALPGPWPQIL
                     TADTRGILRNHPLTLPLTRAIEDSTKQGRRAALIVSRRASALACDDCGAILRCPDCGV
                     PRALSRDRRELRCPLCARADAPPDHCGECGGHRLSPFGWDAERVQTSVVRRFPRLTVS
                     RQNLEAQVVIGTPALLRTLPTRSVGCVGFIALDAMLRVPDFRAGERTWELLWAAAEAV
                     MPDGRVIVQTQHPEHYAVRTVQAQDRAAFYKEELRFRAELGYPPFRRLCEVSARARED
                     SRAHALADECAAALRGIADLTVYPPASASPAGARNRRWRFVVKGPADLPHRLAPALAP
                     LLGKRRGAAGMVEVEMDPQANA"
     gene            5238..5759
                     /gene="def"
                     /locus_tag="VNU03_13130"
     CDS             5238..5759
                     /gene="def"
                     /locus_tag="VNU03_13130"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWP75458.1"
                     /translation="MALLTVKLYGDPVLRQVAAPVREITPEIKRIIADMTETMWHQVG
                     IGLAAPQVGLSHRIIVMDDSKGGVQALINPVIESRSGTIREEEGCLSLPGIFADVERS
                     RSITVSALDADGKPFSFEASGLKARVVQHEMDHLDGVLFIDRLPPVTRDRIKKKIQKE
                     GFPKETAHHAFAL"
     gene            5779..>6717
                     /gene="fmt"
                     /locus_tag="VNU03_13135"
     CDS             5779..>6717
                     /gene="fmt"
                     /locus_tag="VNU03_13135"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWP75459.1"
                     /translation="MFYGTPAFALPTLEGLLAHHHVVAVITQPDRPAGRGRPLTASLV
                     KRRAGEAGVPVLQPPRLRDPGWPERLAEYRADVAVVAAFGQILPKTVLDVPARGSINV
                     HASVLPRYRGAAPIAWAIIRGETESGITTFQMDPGMDTGAMLLREPTAIGPDETAGEL
                     SARLAGIGARLLLRTLEEIDTLRRIPQDAAQATLAPRLKKEDGWLRPAETARDLVNRI
                     RGCNPWPGAALLTPVGRLLIWRARALPGNTTAPPGTLTPLSPPGRGQGEGEGPETSGQ
                     LGIATGSGILLPIEVQPDNRKAMPWVDFLRGARLEPG"
BASE COUNT          968 a         2550 c         2349 g          850 t
ORIGIN      
        1 tggccgctgc tcgagaaggc gctgggcggg gcgctcgccc tcatggtgga gcgtcgggag
       61 acggagggcg ccgcgctcgc gcaggagctg ggcgggctcc gggaggccct cgcgggccac
      121 gtcgagacgg tcgcccggcg cgcccccgcg gcggtggagc gtcgcaccgc gcgactgcgc
      181 gagcggatgc agacgatgct gggcgacacc cagatcgacc ccgcgcgcct ggccaccgag
      241 gtcgcggtgt gggccgatcg gaccgacgtc agcgaggagc tggcgcggct gcgcgcgcac
      301 ctcgcccagc tcgccgcgct cctggccggc gacgagccgg tggggcggac gctcgacttc
      361 ctgatccagg agatcaaccg ggaggtgaac acggtcggct ccaaggcgga cgacctggag
      421 atctcccaga ccgtgatcgc ggcgaaggcc accctcgaga agatgcgcga gcaggtgcag
      481 aacatagaat gatgcgggac tgatgcagct catcaagcgc cgcggcacgc tggtggtcgt
      541 ctcggccccg tcgggggcgg gaaagaccac gctctgccac gaggtgcgca cgctcgtgcc
      601 cgatctgtac tactcggtct cctacaccac ccgctcgccg cgccacgacg agacgaacgg
      661 gaacgacttc ttcttcgtga ccgagggcgc gttcaccgcg atgcgggcgc gcgacgagtt
      721 cgcggagtgg gccgaggtgc acggccacct ctacggcacg ccggccaagg cgctcgagag
      781 cgcgctcggc cgcggcctcg acgtgctcct cgacatcgac acccacggcg cccgccagct
      841 ccgccagcgc tacccggagg cggtctcggt gttcatcatg gccccgtcca tggccgagct
      901 ggaggctcgc ctgcgcgagc gtaacagcaa ctcgccgggc gacatcgccc ggcggctcag
      961 ccgcgccaag gaggagatcg cggcgtggcg gcagtacgac tacctgatca tcaaccgtga
     1021 cgtgaaggac gcggtggacc agctcgcgac catcatccag gccgagcgct gccgcacgag
     1081 ccgattgacc ctgcgtttcc ccgacatgga ggtgccggcg tgatgtcgtt cccatccctc
     1141 gagaagtccc tcgacaacgt gtccaatcgc tacatgctgg tggtgctcgc cgccaagcgt
     1201 gcccgccagc tgaaccgggg cgccaaggcc caagtcgaca cccggcacaa gaagcccacc
     1261 agcaccgcac tcgaagagat cgccgacgcc cgcatcggct accgagtcaa ggaagaagac
     1321 accccaaagc tctagaacca agcggagcca atctcagacg ggtgtccgcc actgggggag
     1381 tgcgggggcc atttctaggc ccccgcagct gaagggctca aagacttaaa cgactgacag
     1441 caggaacggc tgacagcaag aacggctgac agcaagaacg gccgacagca gaagccatga
     1501 gcctaaacga caaagacctg atcctcggcg tcaccggcag catcgccgcc ttcaaggcgg
     1561 tctacctgct gcgcgagctc acgcgtctgg gcgcccgggt cacggtggtc accaccgcgc
     1621 acgcggacaa gttcatcggt cccctcacct tccggacgct ctccggccgc ccggtgctct
     1681 ccgatctgtt cgatccgcag agcccggagg cggtcgagca cgtggccctg gccgagcgcg
     1741 cccacgccct cgtggtcgcg ccggccaccg ccaacctcct ggcccgcgcc gcgcacggca
     1801 tcgccgacga ctttctgacc acgctgctgc tggccgcgcg gtgtccggtg ctgatggccc
     1861 ccgcgatgga cggcggcatg tgggatcacg ccgcggtgac cgccaacgtc cgcaccctgc
     1921 gcgagcgcgg ggtcaccgtg ctcgagcccg acgcgggcgc gctcgcctcc ggcctgtccg
     1981 ggcgcgggcg cctgccggag gtggacgcca tcgtggaggc actcgagcgc gtgctcgccc
     2041 cgcggcgcga cctggccggc gagcgcctgc tcgtgacctc gggtccgacc cgcgagccga
     2101 tcgacccggt gcgctacatc tcgaaccgct cctccggcaa gatgggccag gcagtggcca
     2161 ccgcggccct gcggcgcggc gccgaggtga ccctcatctc ggggcccacc gcgctcaccc
     2221 cgccgcccgg cgcggtctac gtgccggtcc agaccgcgga ggacatgcgc gaggccgcgc
     2281 tccagcatct cgagcgcgcc accatcgtca tcaaggcggc cgcggtcggc gactaccggg
     2341 tcaagcggcc gagcgcgacc aagatcaagt cgaagaagga cgaggggctc gcgctggatc
     2401 tggtggcgaa cccggacatc ctgcgcgagc tggccgcccg caagggccgg gccttcctgg
     2461 tgggcttcgc ggccgagacc aacgacgtgc gcgccaacgc ggcggccaag ctcgaggcca
     2521 agggcgtcga tcttctcgtc gccaacgacg tgagcgtgca gggcatcggc ttcgaggccg
     2581 acgacaacca ggtgacgctg ctggatcgct ggggcggcgt ggccgacctt ccccggatga
     2641 gcaagctcga ggtggccgac gccatcctcg accgagtgct cggcctgcgc tcggcgcagc
     2701 gcgccgggac tcccgaccgc accgcgcgct agccggatga gcgacccgcg ggaggccgtg
     2761 gccctggccc tgcgcgggct cgccgacacc ctccggcacc accgcgacct ggggctccgc
     2821 gacctgaccc tcgcctccgt gcccttcccc gatccggccg acgccctgcg cgagcaggag
     2881 cgcgcccgtc agggctgcca gctctgcaag ctctgcaaga gccgcacgaa cgtggccttc
     2941 ggctcgggcc acgcgcgggc acgcctcatg gtggtgggcg aggggccggg cgaggaggag
     3001 gacaagcagg gcctgccctt cgtgggacgc gcgggccagc tcctcaccaa gatgctcgcc
     3061 tcggtgggct tcgatcgcga gcgcgactgc tacatcgcca acgtggtgaa gtgccggccg
     3121 gagcgcaacc gcaacccgga gccggacgag gtcgcggcgt gcagcccgtt cctgatggcc
     3181 cagatcgaca cggtgcgccc ccacgtcatc ctggccctcg gcaacttcgc ggcccagacc
     3241 ttgatcggca ccaaggaagg catcacgcgg ctgcgcggca agacgtacgc gttccgcacc
     3301 gcggtgctgg tgccgacctt ccatcccgcg ttcctgctgc gcaacccggg ccaggagttc
     3361 aagcgcatgg cctgggacga cctgaagctg gcgcggcgcg aatacgacgc gcgcgcgacc
     3421 gacggtgggc ggaccgacgg ttgatcgcgg atatcgtctt cgatatcccc atcgaccacg
     3481 cgttctccta cgcggtcccg gcgggcctcg acgtgacgcg cggccagcgg gtgagcgcgc
     3541 cgctccacgg ccggagccgc gtgggggtgg tggtcgggct ccgtgaaggc gacgcgaccg
     3601 cgctgaagcc gctccagcgc gcggtcgagc cggtgccggt gctgtcggac gccgcgctcg
     3661 agctgacacg ctgggccgcg ggcgagagcc tctcgtcgtg gggctccacc ctcctgtccc
     3721 tgctgccgcc cgcgccgcgg ccctccgcgg ccgagaccgt ctcgccgccc gcggatccgc
     3781 cgccgagccc cgcgcacgag cccgagctct gggtggatca tcgccgcgag gcccggctcg
     3841 ccgagcatct cgagcgggag accggcgccg cgctcgtcat cacgcccgac cgcgaggccg
     3901 ccgcgcgctg ggcgcgccgg ctcgacgcgg tccgcctcga cagcggggtg cccgagcccg
     3961 agcggcgccg cgcctggttc gcggcctcgc gcgggcgcag ccgcgtggtg gtcggcaccc
     4021 gctccgccct gctcgccccg ctgccgcccc cggccacgct ggccctgatc gacgagcacg
     4081 acccggccca caagccgccg ggcgcgccgc gcctgcactc gcgggacctg ctcctgcgcc
     4141 gcgccgcgct cgacgggagc cggctcctgc tgctctccgc gacgccgtcg gtggagacct
     4201 ggtggcgcgc gcaggacgga cagatcgccc gcgccgaggc cctgcccggc ccctggcccc
     4261 agatcctgac cgcggacacc cggggcatcc tgcgcaatca cccgctgacg ctccccctca
     4321 cccgcgccat cgaggacagc acgaagcagg gccgccgcgc ggcgctgatc gtcagccgcc
     4381 gggcctcggc cctcgcctgc gacgactgcg gggccatcct gcgctgcccg gactgcggcg
     4441 tgccgcgcgc cctctcgcga gaccgccgcg agctgcgctg cccgctctgc gcgcgggccg
     4501 atgccccgcc cgatcactgc ggcgagtgcg gcggacaccg actatcgccc ttcggctggg
     4561 acgccgagcg cgtgcagacc tcggtggtcc gtcgctttcc gcgcctcacc gtctcgcggc
     4621 agaacctcga ggcccaggtg gtgatcggca ccccggcgct gctgcgcacg ctccccacgc
     4681 ggtcggtggg ctgcgtgggc ttcatcgccc tggacgcgat gctccgcgtg ccggacttcc
     4741 gggccggcga gcgcacctgg gagctgctgt gggcggcggc ggaagcggtg atgcccgacg
     4801 gacgcgtgat cgtccagacc cagcaccccg agcactacgc ggtccggacc gtccaggcgc
     4861 aggaccgcgc cgcgttctac aaggaggagc tgcgcttccg ggccgagctg ggctacccgc
     4921 ccttccgccg cctctgcgag gtctcggccc gggcccgcga ggacagccgg gcgcacgcgc
     4981 tggccgacga atgcgcggcg gccctgcgcg gcatcgccga tctgacggtc tatccgcccg
     5041 cgtcggccag cccggccggc gcgcgcaacc ggcgctggcg attcgtcgtc aaggggcccg
     5101 ccgacctgcc gcatcgcctc gcgcccgcgc tggcgccgct gctcgggaag cgacgcggcg
     5161 cggccggtat ggtagaagta gagatggacc cgcaggcgaa tgcctgaggt ggaagcggtc
     5221 cagaggagac gcgcccagtg gccctgctga cggtgaagct gtacggcgac ccggtgctgc
     5281 gacaggtggc cgccccggtc cgcgagatca cccccgagat caagcggatc atcgccgaca
     5341 tgaccgagac catgtggcac caggtgggca tcggcctggc cgcgccacag gtcggcctgt
     5401 cccaccggat catcgtgatg gacgacagca agggcggggt ccaggccctc atcaacccgg
     5461 tcatcgagtc ccggagcggg acgattcgcg aagaggaggg ttgcctgtcc cttcccggca
     5521 tcttcgcgga cgtggagcgg agccggtcga tcaccgtctc ggcgctcgac gcggacggca
     5581 agccgttctc gttcgaggcg agcgggctca aggcgcgcgt ggtccagcac gagatggacc
     5641 acctggacgg cgtgctgttc atcgatcgcc tgcccccggt cacgcgggac cgcatcaaga
     5701 agaagatcca gaaggaaggc ttccccaagg agaccgcgca ccacgccttc gcgctctagc
     5761 gagtcggggc tgcgcgtcct gttttacggc accccggcct tcgccctgcc gaccctcgag
     5821 ggcctgctcg cacatcacca cgtcgtggcc gtgatcaccc agcccgatcg gccggcggga
     5881 cgcggccgcc ccctgaccgc ctcgctcgtg aagcgccgcg cgggcgaggc cggcgtgccg
     5941 gtcctccagc cgccgcggct gcgcgatccg ggctggcccg agcgcctcgc cgagtaccgg
     6001 gccgacgtcg cggtggtcgc ggccttcggc cagatcctgc ccaagaccgt gctcgacgtg
     6061 ccggcccgcg gatcgatcaa cgtccacgcc tccgtcctgc cgcgataccg cggggccgcg
     6121 cccatcgcgt gggccatcat ccgcggcgag accgagagcg ggatcaccac cttccagatg
     6181 gacccgggca tggacaccgg cgccatgctg ctgcgcgagc cgaccgcgat cggccccgac
     6241 gagaccgcgg gcgagctgtc ggcgcgcctg gccgggatcg gcgcccggct cctgctccgc
     6301 accctcgagg agatcgacac cctgcgccgc atcccgcagg acgcggcgca ggccaccctg
     6361 gccccgcggc tcaagaagga agacggctgg ctccggccgg ccgagacggc gcgcgacctc
     6421 gtcaaccgga tccgcggctg caacccgtgg ccgggcgccg ccttgctcac gccggtgggc
     6481 cgtctcctca tctggcgcgc ccgggccttg cccggcaaca cgaccgcgcc cccgggcacg
     6541 ctgacccccc tctcccctcc ggggagaggg cagggtgagg gtgagggacc cgagacttcg
     6601 ggccagctcg gcatcgcaac cggctccggc atcctcctcc cgatcgaggt gcagccggac
     6661 aaccgcaagg ccatgccctg ggtcgacttc ctccgcggcg cgcgcctcga gccgggc
//
LOCUS       DATMCV010000570         3567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_497417, whole genome shotgun sequence.
ACCESSION   DATMCV010000570 DATMCV010000000
VERSION     DATMCV010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497417"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VNU03_13140"
     CDS             complement(<1..1130)
                     /locus_tag="VNU03_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75460.1"
                     /translation="MDAEKIDLMLRMLPDGVSRRDFLRTAAAGAAGAAGALTVPKRAR
                     AQGAKKGGTLVYGMEGPSDILDPQATGCWLTYRVTFQMFEGLLAEDLTKSNVAIPPVV
                     PKLAESYTTAKDGLSRTFKLRKGVKFHDGTPFNAAAAVFSWERMYKKDAPHFYARANS
                     YTSYVVEYITGAEAVDEYTLKLTFSKPYSEWERMTLQSWGEPMMVSPTQVKKTGNEKF
                     ADAPVGTGPFKFVENVPGDRIVLERFKDYWGPQANVDKLVFRRLDDPAARVAALRTGE
                     VDFILAPPPDEVEALRKEKFQVLQSNAPHIWYWHLNMKDEKFKDVRVRKALQMSVDKD
                     RMCKDLLRGTAKPAWSMISPATVAYDPKYKPYSYDPEKAKALL"
     gene            complement(1162..2064)
                     /locus_tag="VNU03_13145"
     CDS             complement(1162..2064)
                     /locus_tag="VNU03_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75461.1"
                     /translation="MATTSTALTVTPAASRARRPRGALAQSARAFARDRQAMVGLLII
                     AIIGGAALAAPLISPWDPLTSAAVERLSPIGTPGHLLGTDEQGRDILSRLIWGGRASL
                     LVGILPTVTAGLMALGLGLLAGYLGGALDHVIMRALDVFFAFPLVLLAIAIVGVIGPG
                     MDNQMFALTVVLVPYSTRVVRTATLSVKPLEFVEAARALGASGGRLVGRHLLPNVLPP
                     LLVYTSTLVGMMIVASAGLSFLGLGVQPPTPDWGVMVGNGRLVLYRAAHVATIPGIVI
                     VVAALAFNFVGDGLREALDPRARR"
     gene            complement(2067..3017)
                     /locus_tag="VNU03_13150"
     CDS             complement(2067..3017)
                     /locus_tag="VNU03_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75462.1"
                     /translation="MIVYVLQRLLGLVPVLFGITVVIFLTMKLIPGDVARALLGPMAT
                     DQSLAQLRHALGLDEAIYVQYVKWLWRALHGDLGLSPIVHKPVVELLLPKFGNTLILA
                     GASFALAVVGGVAVGVLAASRQQTVVDRATMGASLVIGNMPPFWLGLVLIVFFAINLR
                     WLPSLGMYSSRGAGGFSDLLAHLIMPAIATAAAPAAIIARMTRASVLEILGQDYIKVA
                     RSKGLSERAVLGLHALRVAWPPILTISGLQLGYLLGGAVFTEEVFGWPGLGRQLVSSV
                     LARDVQAVQGAALFIALSFVMVNLVVDLLNLYLDPRSRPA"
     gene            complement(3014..>3567)
                     /locus_tag="VNU03_13155"
     CDS             complement(3014..>3567)
                     /locus_tag="VNU03_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75463.1"
                     /translation="ARGVSCGQWTFWRTMVTVTAETSVSSSLDETWTALGRRNTYFYF
                     PGVSPATARAAGPLPHTLDLPIVDRQEQAATLSVGRAGKAGHRERRFTLRGALVTIAG
                     RWQLEPGDAGVRVRLTLDYQIAPELKTHAVNTLRSRSPLPIRTDADAILSRAVDEFFE
                     TRFAEQAAAYCEQVRERLSGQPA"
BASE COUNT          553 a         1208 c         1316 g          490 t
ORIGIN      
        1 agcagcgcct tggccttctc ggggtcgtac gagtagggct tgtacttcgg gtcgtaggcg
       61 acggtggcgg gggagatcat gctccacgcc ggcttcgcgg tgccgcgcag cagatccttg
      121 cacatccggt ccttgtcgac cgacatctgg agcgccttgc gcacccgcac gtccttgaac
      181 ttctcgtcct tcatgttcag gtgccagtac cagatgtggg gcgcgttcga ctgcagcacc
      241 tggaacttct ccttgcggag cgcctccacc tcgtcgggcg ggggcgccag gatgaagtct
      301 acctcgccgg tgcggagcgc ggccacccgc gcggccggat cgtcgaggcg ccggaagacc
      361 agcttgtcca cgttggcctg ggggccccag taatccttga agcgctcgag cacgatccgg
      421 tcgccgggca cgttctccac gaacttgaag ggcccggtgc cgaccggggc gtcggcgaac
      481 ttctcgttgc cggtcttctt gacctgggtc gggctcacca tcatcggctc gccccagctc
      541 tggagcgtca tgcgctccca ctccgagtac ggcttgctga aggtgagctt cagcgtgtac
      601 tcgtcgaccg cctccgcccc cgtgatgtac tcgaccacgt aggaggtgta gctgttggcc
      661 cgggcgtaga agtggggcgc gtccttcttg tacatgcgct cccaggagaa gaccgcggcg
      721 gccgcgttga acggcgtgcc gtcgtggaac ttgaccccct tgcgcagctt gaaggtgcgg
      781 gagaggccgt ccttcgcggt agtgtaggac tcggcgagct tggggaccac gggagggatc
      841 gccacgttgg acttggtcag gtcctccgcg agcaggccct cgaacatctg gaaggtcacc
      901 cggtaggtga gccagcagcc ggtggcctgc gggtccagga tgtcggacgg cccctccatg
      961 ccgtagacga gggtgccgcc cttcttggcc ccctgcgcgc gcgcccgctt gggcaccgtg
     1021 agcgccccgg ccgctcccgc cgcgccggcg gcggcggtgc ggaggaagtc ccgccggctc
     1081 acgccgtcgg gcagcatgcg cagcatcaag tcgatcttct cggcgtccat gactcttctc
     1141 cttgggctcg acccgggggg cctaccggcg cgcgcgaggg tcgagggcct cgcgcagacc
     1201 gtctccgacg aagttgaacg cgagcgcggc gacgacgatg acaatgccgg ggatggtggc
     1261 cacgtgggcg gcgcggtaga gcacgagccg gccgttgccc accatgacgc cccagtccgg
     1321 ggtggggggc tgcacgccca ggcccaggaa gctcaacccg gccgaggcca cgatcatcat
     1381 gcccaccagg gtgctggtgt agaccaggag cggcggcagc acgttgggca acaggtggcg
     1441 cccgacgagg cgcccgccgc tggcgcccag cgcgcgggcg gcctccacga actcgagcgg
     1501 cttgaccgag agcgtcgcgg tgcgcaccac gcgggtactg tacggcacga ggaccacggt
     1561 gagggcgaac atctggttgt ccatgccggg gccgatcacc cccacgatcg cgatggcgag
     1621 cagcaccagc gggaacgcga agaagacgtc gagcgcgcgc atgatgacgt ggtcgagcgc
     1681 gcccccgaga tagccggcca ggagccccag gccgagggcc atcaggcccg cggtcacggt
     1741 gggcaggatg cccaccagca gcgaggcgcg cccgccccag atgaggcggc tcaggatgtc
     1801 gcgcccctgc tcgtcggtgc cgaggagatg ccccggggtg ccgatgggcg agagccgctc
     1861 gacggcggcc gaggtgaggg gatcccacgg gctgatgagc ggcgcggcca gcgcggcccc
     1921 gccgatgatc gcgatgatca gcagccccac catcgcctgg cggtcgcgcg cgaacgcgcg
     1981 cgcggactgc gcgagggcgc cgcgggggcg gcgggcgcgc gacgcggccg gggtcacggt
     2041 gagcgcggtc gaggtggtgg ccacggtcag gccggccgcg agcgcgggtc gaggtagagg
     2101 ttcagcaggt ccaccaccag gttcaccatc acgaacgaga gcgcgatgaa gagcgcggcg
     2161 ccttgcaccg cctgcacgtc gcgcgcgagc accgaggaga cgagctggcg cccgagcccc
     2221 ggccagccga agacctcctc ggtgaagacc gcgccgccca gcaggtagcc gagctgtagg
     2281 cccgagatgg tcaggatggg cggccaggcc acccgcagcg cgtgcaagcc cagcaccgcg
     2341 cgctcggaga gccccttgga gcgcgcgacc ttgatgtagt cctggccgag gatctcgagc
     2401 accgaggcgc gcgtcatgcg cgcgatgatg gcggcggggg ccgccgcggt ggcgatcgcg
     2461 ggcatgatca ggtgggcgag caggtcggag aaaccgccag ctcctctgga ggagtacatg
     2521 ccgaggctcg gcagccagcg caggttgatc gcgaagaaca cgatcagcac caggccgagc
     2581 cagaagggcg gcatgttgcc gatcaccagc gaggcgccca tcgtcgcgcg gtcgaccacc
     2641 gtctgctgcc gcgaggccgc gagcactccg accgcgacgc cgcccaccac cgccagcgcg
     2701 aagctggcgc cggccaggat gagggtgttg ccgaacttgg ggaggagcag ctcgaccacc
     2761 ggcttgtgca cgatggggga gagcccgagg tcgccgtgca gggcccgcca cagccacttc
     2821 acgtactgca cgtagatggc ctcgtcgagc ccgagggcgt ggcggagctg ggcgagggac
     2881 tggtcggtgg ccatcgggcc cagcagcgcg cgcgccacgt cgcccgggat cagcttcatg
     2941 gtgaggaaga tcaccaccgt gatgccgaag agcaccggca ccaggcccag caggcgctgg
     3001 agcacgtaga cgatcatgcg ggctgaccac tcaggcgctc gcgcacctgc tcgcagtagg
     3061 cggcggcctg ctcggcgaag cgcgtctcga agaactcgtc gaccgcccgg ctcaggatgg
     3121 cgtccgcgtc ggtccggatc gggagcgggc tccggctgcg cagggtgttc accgcgtgcg
     3181 tcttcagctc cggcgcgatc tggtaatcga gggtgaggcg cacccgcacg cccgcgtcac
     3241 cgggctcgag ctgccagcgc cccgcgatgg tgacgagcgc gccgcgcagc gtgaagcggc
     3301 gctcgcggtg cccggccttg cccgcccgtc ccaccgacag cgtggcggcc tgctcctgcc
     3361 ggtccacgat gggcaggtcg agcgtgtggg gcaatggacc ggccgctctc gcggtcgcgg
     3421 gcgagacgcc ggggaagtag aagtacgtat ttcgccggcc gagcgcggtc caggtctcgt
     3481 ccagggagga cgacacggac gtctcggcgg tcacggtgac catcgttctc cagaaagtcc
     3541 actggccgca cgacacgccg cgggccc
//
LOCUS       DATMCV010000571         2169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_365166, whole genome shotgun sequence.
ACCESSION   DATMCV010000571 DATMCV010000000
VERSION     DATMCV010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365166"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VNU03_13160"
     CDS             complement(<1..934)
                     /locus_tag="VNU03_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWP75464.1"
                     /translation="MKHRAPASSPRGRRRPHVWELAAGLVALLLFSVEPAPAQRQYVV
                     AFANLTEEPGVTVEGTGFTGRDIREGFVLAARAHPVEMVYYDNQRDDARALQNADAAI
                     ARKVDLYIQYHQGAANAAIGEKLKAAGIRVLALNYPVPGAPLYTVDNAAAGRVAGEAL
                     AQFALRSWRGQPTVAAVVGNLSATADRVPERAKGVTDVLAQRLPAVRVTSLDTRGNPA
                     QVGPLLGKFLAAHPTGKLLLAATDDTTALALKSAVEAAGRVHDTAIVSHGVDRTIHGG
                     MNDRKEIDPNNRGSIVIGSVAFYLDRYGYEVLPIA"
     gene            complement(958..1947)
                     /locus_tag="VNU03_13165"
     CDS             complement(958..1947)
                     /locus_tag="VNU03_13165"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWP75465.1"
                     /translation="MAALMVFAFGSAAFAQTDCKALMPASPWGPNDQTGATNRVTSAV
                     TKAAAAEIQDGKVIAMSYPLTDGVPLFGSRFTKTILTATTLAPGGALGENNLTYMEDT
                     WLSQSHVGTHLDGMGHIGRKDCYFNQTPMGKFITQNNMTKLGLEHLKPFATRGVVIDM
                     VKVYQQANKLKTNPACKKPCIDKGTVFSAADLQAGLKMYNVTLREGDIVVIHTGWGDL
                     FEQFPAQNALYNSGEPGLGKEAAAWLISQKIVAVGTDTWAVEVIPGESPKEAFIVHQM
                     LLTDNGIHIIENVRTDLIAAEAAASNRATFFFSMTVPKGVGMTGNFVGIEGIR"
BASE COUNT          302 a          713 c          784 g          370 t
ORIGIN      
        1 tcgcgatcgg caggacctcg tagccatagc gatccaggta gaaggccacc gagccgatca
       61 cgatgctccc gcggttgttc ggatcgatct ccttgcggtc gttcatgccg ccgtggattg
      121 tccggtcgac gccgtggctg acgatggcgg tgtcgtggac gcggccggcc gcctccaccg
      181 ccgacttcag cgccagcgcg gtggtgtcgt cggtcgcggc gaggagcagc ttgccggtcg
      241 ggtgcgcggc caggaacttc ccgaggagag ggccgacctg ggccgggttg ccccgggtgt
      301 cgagcgaggt gacccgcacc gcgggcaggc gctgggcgag cacgtcggtg acgcccttgg
      361 cgcgctcggg cacccgatcc gcggtggcgg agagattgcc gaccaccgcc gccaccgtgg
      421 gctgcccccg ccacgagcgg agcgcgaact gagcgagggc ctcgcccgcg acacggccgg
      481 cggccgcatt gtccaccgtg tagagcggcg cgcccggaac cggatagttc agggcgagga
      541 cgcgaatccc ggccgccttc agcttctcgc cgatcgctgc gttggcggcg ccctggtggt
      601 actggatgta gaggtcgacc ttgcgcgcga tggccgcgtc cgcgttctgg agcgcgcgcg
      661 cgtcgtcgcg ctggttgtcg tagtagacca tctcgaccgg gtgggcccgc gcggccagga
      721 cgaagccctc gcggatgtcg cgtcccgtga agccggtgcc ctccaccgtg acgcccggct
      781 cctcggtcag gttggcgaag gcgacgacgt actgccgctg agccggggcc ggctcgacgg
      841 agaagagcag cagcgcgacc aggcccgcgg ccagctccca aacgtgaggg cgccggcgtc
      901 ccctcgggga ggacgccggc gcccggtgct tcacgagcga tactccggtc gggcgatcta
      961 gcggatgcct tcgatcccga cgaagttgcc ggtcatgccg acgcccttcg ggaccgtcat
     1021 gctgaagaag aaggtggcgc gattgctcgc ggccgcctcg gccgcgatca ggtcggtgcg
     1081 cacgttctcg atgatgtgga tgccgttgtc ggtcagcagc atctggtgga cgatgaaggc
     1141 ttccttcggg ctctcgccgg gaatgacctc gaccgcccag gtgtcggtcc cgaccgcgac
     1201 gatcttctgc gagatcagcc acgcggccgc ttccttgccg aggccgggct cacccgagtt
     1261 gtagagcgcg ttctgggccg ggaactgctc gaagaggtcg ccccacccgg tgtggatgac
     1321 gacgatatcg ccctcgcgca gggtgacgtt gtacatcttg agcccggcct ggaggtcggc
     1381 ggcggagaag acggtgccct tgtcgatgca gggcttcttg caggcgggat tggtcttgag
     1441 cttgttggcc tgctggtaga ccttgaccat gtcgatcacc acgccccggg tcgcgaaggg
     1501 cttgaggtgc tcgagaccga gcttggtcat gttgttctgg gtgatgaact tgcccatcgg
     1561 ggtctggttg aagtagcagt ccttccggcc gatgtgcccc atgccgtcca ggtgcgtccc
     1621 gacgtggctc tggctgagcc aggtgtcttc catgtaggtc aggttgttct cgccgagggc
     1681 gccgcccggg gcgagcgtgg ttgcggtcag gatggtcttg gtgaagcggc tcccgaagag
     1741 cggcacaccg tcggtcagcg ggtaggacat cgcgatgacc ttcccgtcct ggatctccgc
     1801 cgcggcggcc ttggtcaccg ccgacgtcac ccggttcgtc gcgcccgtct ggtcattcgg
     1861 gccccacggg gacgcgggca tcagggcctt gcagtccgtc tgggcgaagg ccgccgagcc
     1921 gaacgcgaac accatcagtg ccgccatcac tgccacgatc gctcttgggc tcataggccg
     1981 ggactccttc tcttcagtga gaggggcacg ccgcgcgtca cggggccgac gccgggggca
     2041 tcgtagtgca gcgcgcacgg ccaggtcaag cgtcgagcgt agcgcggggc gtcaatctca
     2101 ctcggcgagg accttgtgca cgagctggac cgcggccgag ccctcgccca ccgcggacgc
     2161 cacgcgctt
//
LOCUS       DATMCV010000572         5247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_693297, whole genome shotgun sequence.
ACCESSION   DATMCV010000572 DATMCV010000000
VERSION     DATMCV010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693297"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VNU03_13170"
     CDS             complement(<1..314)
                     /locus_tag="VNU03_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75466.1"
                     /translation="MSIRLGLVVGLLFAILVTYLASLNPGRAHLALGGDWAVDVPLMG
                     LVVGVFGAGAVLALVLGWLRDLTRSYACGPVAVAAPRAPLRPDSAPLRAAEPAPAPAR
                     AAR"
     gene            complement(331..696)
                     /locus_tag="VNU03_13175"
     CDS             complement(331..696)
                     /locus_tag="VNU03_13175"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HWP75467.1"
                     /translation="MKKDRLVHYRKKLEEKHGQLVEEVGRNVLYGKGPEDDSIKDLGD
                     QASSAYNREFLFELGNGDRRLLKEVVLALQKISEGGFGDCERCGEPISEKRLEALPFA
                     RYCIECQRAVEEEERTAAR"
     gene            complement(778..1131)
                     /gene="rsfS"
                     /locus_tag="VNU03_13180"
     CDS             complement(778..1131)
                     /gene="rsfS"
                     /locus_tag="VNU03_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWP75468.1"
                     /translation="MATLSAEAVVRAAAHAALEKKAIDLAVLDLQGLSSVADFFLVCS
                     ARSTPQADTIAEAIRAALRAKGVRPRHNEGSAESGWLLLDYGDVVMHVFLEETRGFYA
                     LDRLWGDAPLVSVEG"
     gene            complement(1131..1760)
                     /gene="nadD"
                     /locus_tag="VNU03_13185"
     CDS             complement(1131..1760)
                     /gene="nadD"
                     /locus_tag="VNU03_13185"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWP75469.1"
                     /translation="MFGGSFNPIHFGHLLVADEVCETLRLDRLLLVPAAQPPHKPLTE
                     LAPAADRYRMTALAVREHPRFEVSDVEVARSGPSYTVDTLTALQDRGRLHLIIGSETF
                     LDLLNWREPQRVVTLARLVVIPRNGMDFDPEAPAAVKVLRELGLPGFAPASGESPDGA
                     APLLLHAASLPISGSDLRRRAREGRSLAFRMPDAVAGYLREHHLYRPEA"
     gene            complement(1783..3039)
                     /locus_tag="VNU03_13190"
     CDS             complement(1783..3039)
                     /locus_tag="VNU03_13190"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWP75470.1"
                     /translation="MDAAAHVETKAKAAKEASRILALASTRVKNEALAQMARGLEERT
                     GAILDANRHDLERARTAGHARAFVERLTLTEGRVEEMAAGLRHVAGLPDPVGETVETI
                     RRPSGLEIARVRVPLGVIGFIYESRPNVTADAAGLCLKSGNAVLLRGGSEALESNTLL
                     AQILGKAVEKAGLPPEVIQAVETADRAGVMAMLTLDRYLDLIIPRGGEEFVRLVAERA
                     TVPVLKHDKGLCHLYVDDGADLDMALAVAVNAKVQRVSVCNSLETLLVHAAIAPALLP
                     RLAARLAEVPVEVRADERARALMPGARAAEAADWDAEYLDYILAVRVVEDLDEAVAHI
                     RRHGSGLAESIVTRDVRRARRFTQEVDAAAVLVNASTRLVDGSQFGMGAEMGISTSRV
                     HARGPVGVRELTTTKFVVWGDGHIRE"
     gene            complement(3070..4212)
                     /gene="proB"
                     /locus_tag="VNU03_13195"
     CDS             complement(3070..4212)
                     /gene="proB"
                     /locus_tag="VNU03_13195"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWP75471.1"
                     /translation="MADERAMGSRRPLSHARRVVVKVGSGLVTTPGEGPSSEHIGRLA
                     TDISTLVHDRHEVALVTSGAIATGMARLGLKARPRSIPEKQAAAAVGQSALMWEYEQA
                     FKKHGIAVGQVLLTGQDISDRSRYLNARNTLLALLAFGVLPIVNENDTVAVDEIKVGD
                     NDNLAALVAHLIDADLLVLLTDVDGLYTGDPRRDPAARRLETVEAITDEIQRMVYDAS
                     ASVSVGGMSTKLQAAQKAGASGIPMVIASGREPGTLPRLLKGEPVGTYFQPRDDRLAA
                     RKRWIAFAVPPQGRLTVDAGAKKALTERGKSLLPSGLVEVHGQFDAGDVVALTEADGQ
                     EFARGLVNYDTDELRRIRGAKTADIERTLGYKGVDEVVHRDNLVIL"
     gene            complement(4173..5192)
                     /gene="obgE"
                     /locus_tag="VNU03_13200"
     CDS             complement(4173..5192)
                     /gene="obgE"
                     /locus_tag="VNU03_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWP75472.1"
                     /translation="MFVDEIDVFVKGGDGGAGCISFRREKYVPRGGPDGGDGGDGGSV
                     ILEADPAITTLLDFHYQRHYTAERGTHGQGSNKHGRSGEDTVLRVPLGTTVSERDGRL
                     PLGDLTERGQRLVVARGARGGRGNARFATSTNRAPRRADLGRSGEEHWIHLELKLLAD
                     VGVVGFPNAGKSTLVSRLSAAKPRIADYPFTTLEPSLGIVRAGDDRTFVIADLPGLIP
                     GAAHGKGLGIRFLRHTERTRLLVHLIDLDPSTGRDPLADYHAIQDELRAYSEELAARP
                     QIVAVNKIELPGTEESRAAVERFCAQHGFSFHAISAVTGVGLPGLVHEMARWLTSEPW
                     VPAAR"
BASE COUNT          725 a         1893 c         1897 g          732 t
ORIGIN      
        1 cgcgcggcgc gggccggcgc gggcgccggc tcggcggcgc ggagcggagc tgagtccggg
       61 cggagcgggg cccgcggcgc cgcgaccgcc accggcccgc aggcgtagga gcgcgtcagg
      121 tcccgcagcc atccgagcac cagcgcgagc accgcgcccg ccccgaagac ccccaccacc
      181 agacccatga gtggcacgtc gaccgcccag tcgccgccga gggccaggtg cgcgcggccc
      241 gggttgagcg aggcgagata ggtgaccagg atggcgaaga gcagaccgac gacgaggccg
      301 agacggatcg acacggaggg aacgggggcg ctagcgggcg gcggtgcgct cttcctcttc
      361 gacggcgcgc tggcactcga tgcagtagcg cgcgaacggc agcgcctcca ggcgcttctc
      421 ggagatcggc tcgccgcagc gctcacagtc gccgaagccg ccctcgctga tcttctgcag
      481 ggccagcacg acctctttca ggagccgccg gtcaccgttg cccagctcga agagaaactc
      541 gcgattgtag gcagaggagg cctggtcgcc caggtccttg atggagtcgt cctcgggacc
      601 cttcccgtag agcacgttcc tacccacttc ctccacgagc tggccgtgct tctcctccag
      661 cttcttgcgg tagtggacca agcgatcttt cttcatcgcg ccctcctggc tgcccgcggc
      721 ttcgccgcta gtcgcgcagg ctaccacggg gtccagggca aagcaatccg ggaagcctca
      781 gccctccacc gacacgagcg gagcgtcgcc ccagaggcga tcgagggcgt agaatccgcg
      841 ggtctcctcg aggaagacgt gcatcaccac gtcgccgtag tcgagcagga gccagccgct
      901 ctcggcgctc ccctcgttgt ggcgcgggcg cacccccttc gcgcgcagcg cggcgcggat
      961 ggcttccgcg atggtgtcgg cctggggggt ggagcgcgcg ctgcatacga ggaagaagtc
     1021 cgcgacgctc gacagtccct gcagatcgag caccgcgagg tcgatcgcct tcttctccag
     1081 cgccgcgtga gcggcggcac ggacgacggc ttccgcggac agtgtggcca tcacgcctcc
     1141 ggacggtaga gatggtgttc gcgcaggtac cccgccaccg cgtcgggcat ccggaacgcc
     1201 aggctccggc cctcgcgggc gcggcggcgc aggtccgacc cggagatggg cagggacgcc
     1261 gcgtgcagca gcagcggagc ggccccgtcc ggcgactccc cggacgcggg agcgaagccg
     1321 ggcagaccga gctcccgcag caccttgacc gcggcggggg cctccgggtc gaagtccatg
     1381 ccgttccgcg ggatcaccac gagacgggcg agggtgacga cgcgctgggg ctcccgccag
     1441 ttcagcagat cgaggaaggt ctcggagccg atgatcaggt gcaggcgccc gcggtcctgc
     1501 agcgcggtca gggtgtccac cgtgtaggag ggcccgctcc gcgcgacctc gacgtcggag
     1561 acctcgaagc gcgggtgctc gcgcaccgcg agcgcggtca tccggtagcg gtcggccgcg
     1621 ggggccagct cggtgagcgg cttgtggggc ggctgggcgg ccggcacgag caggagccgg
     1681 tcgagccgga gggtctcgca gacctcgtcg gccacgagca ggtggccgaa gtggatcggg
     1741 ttgaacgatc caccgaacac gccgacgcgc ggctccggac ggctactccc ggatatgccc
     1801 gtcgccccac accacgaact tggtcgtggt cagctcccgc acaccgaccg ggccccgcgc
     1861 gtgcacccgg gaggtggaga tgcccatctc ggcgcccatc ccgaactggc tgccgtcgac
     1921 gagccgcgtc gacgcgttga cgagcaccgc ggccgcgtcc acctcctgcg tgaagcggcg
     1981 cgcgcggcgc acgtcgcggg tgacgatgga ctcggccagc cccgagccgt gccggcggat
     2041 gtgcgccacc gcctcgtcga ggtcctcgac cacccgcacc gcgaggatgt agtcgaggta
     2101 ctccgcgtcc cagtcggcgg cctcggcggc ccgcgcgccg ggcatgaggg cccgcgcccg
     2161 ctcgtcggcc cgcacctcca ccggaacctc ggccaggcgc gcggcgagcc ggggcagcag
     2221 cgcgggcgcg atcgcggcgt ggaccagcag cgtctcgagc gaattgcaca cgctcacccg
     2281 ctggaccttg gcgttgaccg ccaccgccag ggccatgtcg agatccgccc cgtcgtcgac
     2341 gtagaggtgg cagaggccct tgtcgtgctt gagcaccggc accgtcgccc gctccgcgac
     2401 caggcgcacg aactcctcgc cgccgcgcgg gatgatcagg tcgaggtagc gatccagggt
     2461 gagcatcgcc atgaccccgg cgcgatccgc ggtctccacc gcctggatca cctcgggagg
     2521 caggcccgcc ttctcgaccg ccttgccgag gatctgcgcg agcagggtgt tcgactcgag
     2581 ggcctcgctg ccgccgcgca ggagcaccgc gttgcccgac ttcaggcaga ggccggctgc
     2641 gtcggcggtg acgttgggtc gggactcgta gatgaacccg atcacgccga ggggaaccct
     2701 cacccgcgcg atctcgagcc ccgagggccg ccggatcgtc tccaccgtct cgcccaccgg
     2761 gtcgggcagg cccgccacgt gacggagccc ggccgccatc tcctcgacgc ggccctcggt
     2821 gagggtcagg cgctccacga aagcccgggc gtgcccggcc gtccgcgcgc gctccaggtc
     2881 gtgccggttg gcgtccagga tcgcgccggt gcgctcctcc agcccgcggg ccatctgggc
     2941 gagcgcctcg ttcttgacgc gggtcgaggc gagcgcgagg atgcgcgagg cctccttggc
     3001 cgccttcgcc ttcgtctcga cgtgtgccgc cgcgtccatg ctcgcgctct ccgctcgtcg
     3061 gcgaaccggt tagaggatca ccaggttgtc ccggtgcacc acttcgtcca cacccttgta
     3121 gcccagcgtc cgctcgatgt ccgcggtctt cgcgccccgg atgcgccgca actcgtcggt
     3181 gtcgtaattg acgagcccgc gcgcgaactc ctggccgtcg gcctcggtca gggcgaccac
     3241 gtcgcccgcg tcgaactggc cgtggacctc caccaggccc gacggcagca ggctcttgcc
     3301 cctctcagtt aaggccttct tcgcgcccgc gtcaaccgtg agccggccct ggggcgggac
     3361 cgcgaaggcg atccagcgct tgcgggccgc gaggcggtcg tcgcgcggct ggaagtaggt
     3421 gccgaccggc tcgcccttga ggaggcgggg cagcgtgccg ggctcgcgcc cgctcgcgat
     3481 caccatggga atgccggagg cgcccgcctt ctgggccgcc tggagcttgg tgctcatccc
     3541 ccccaccgag accgacgcgg aggcgtcgta gaccatgcgc tggatctcgt cggtgatggc
     3601 ctccacggtc tcgaggcgcc gggcggcggg atcacggcgc gggtccccgg tgtagaggcc
     3661 gtcgacgtcg gtcagaagca cgagcaggtc cgcgtcgatg agatgggcca cgagggcggc
     3721 caggttgtcg ttgtcgccca ccttgatctc gtccaccgcc accgtgtcgt tctcgttcac
     3781 gatcggcagc accccgaacg cgagcagggc cagcagcgtg ttgcgggcgt tgaggtaacg
     3841 cgagcgatcg ctgatgtcct ggccggtgag gagcacctgg cccaccgcga tgccgtgctt
     3901 cttgaacgcc tgctcgtact cccacatgag ggccgactgg ccgaccgccg ccgcggcctg
     3961 cttctcgggg atcgagcggg gacgggcctt caacccgagc cgggccatcc cggtggcgat
     4021 ggctcccgag gtcaccagcg cgacctcgtg ccggtcgtgg accagcgtcg agatgtcggt
     4081 ggcgagccgt ccgatgtgct cgctcgaggg accctcgccg ggagtggtga cgaggccgct
     4141 gcccaccttc accaccacgc gccgcgcgtg gctcagcggg cggcgggaac ccatggctcg
     4201 ctcgtcagcc accgggccat ctcgtgcacc agcccgggca gtccgacgcc ggtcaccgcg
     4261 gagatcgcgt ggaacgagaa gccgtgctgg gcgcagaagc gctcgaccgc cgcgcgggac
     4321 tcctcggtgc cgggcagctc gatcttgttc accgccacga tctgcggccg cgcggccagc
     4381 tcctcggagt aggcgcgcag ctcgtcctgg atggcgtgat agtcggcgag gggatcgcgc
     4441 ccggtcgagg gatcgaggtc gatcaggtgc acgagcagtc gcgtccgctc ggtgtggcgg
     4501 aggaaccgga tgcccagccc cttgccgtgg gccgcgccgg ggatgagccc gggcagatcc
     4561 gcgatcacga aggtgcgatc gtcgccggcg cgcacgatgc cgaggctcgg ctcgagcgtg
     4621 gtgaacgggt agtcggcgat ccggggcttg gccgcggaca ggcgcgacac cagcgtggac
     4681 ttgcccgcgt tcggaaagcc caccaccccc acgtcggcca gcagcttcag ctcgaggtgg
     4741 atccagtgct cctcgccgga ccggccgagg tccgcgcgac gaggggcccg gttggtggag
     4801 gtggcgaagc gggcgttgcc gcgtccgccc cgggcgcccc gggccaccac caggcgctga
     4861 ccgcgctcgg tcaggtcccc gagcggcagc cggccgtccc tctcggaaac ggtggtgccg
     4921 agcgggacgc ggagcacggt gtcctcgccg ctccgcccgt gcttgttgga gccctgtccg
     4981 tgggtgccgc gctcggcagt gtagtgccgc tggtagtgga agtcgagcag cgtggtgatg
     5041 gcgggatccg cctcgaggat cacgctgcct ccgtcccccc cgtcgccgcc gtcggggcca
     5101 ccgcggggga cgtacttctc gcgccggaag ctgatgcaac ccgcgccgcc gtcgccaccc
     5161 ttgacgaaca cgtcgatctc gtcgacgaac atggcgtggt taccgcggtc cggcggggcc
     5221 ggaccggctc aggcgggctg ctgctgg
//
LOCUS       DATMCV010000573         9754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_764338, whole genome shotgun sequence.
ACCESSION   DATMCV010000573 DATMCV010000000
VERSION     DATMCV010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764338"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..460
                     /locus_tag="VNU03_13205"
     CDS             38..460
                     /locus_tag="VNU03_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75473.1"
                     /translation="MRTIIGWSVGAACATVIVTGLSALPAPAQETKTDRREIRQDSRE
                     IRGDRKEIAKDTQEVRGDRKDLEASRQKLREAYKSGDPAAIKAARESYQKSRGSLHGD
                     VKDRRQDARDLRQDKQDRREDVQDLRQDQRQARTRQAK"
     gene            complement(525..1928)
                     /locus_tag="VNU03_13210"
     CDS             complement(525..1928)
                     /locus_tag="VNU03_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP75474.1"
                     /translation="MAEEVVFVPVRRLGDLIRERRLSPVALAETFLDRLERLGPRYNA
                     VVTVTRARALEQARRAEAEIAAGRYRGPLHGIPYGAKDLLATSGGIPTTWGAAPFKAR
                     VFDFDATAIRKLEAAGAVLCAKLAMVELAGGMGYRQPNAAFTGPGVNPWSRDAWSGGS
                     SSGSGSAVAAGLVPFAIGSETWGSILSPAGNCGVAGLRPTYGRVSRHGAMALCWTLDK
                     LGPLALTADDCGLVLEAIAGRDPADPTTVDRPFRHEPAAPAGRQFRFGVLRGATDGCE
                     EAVRENFERSLGVLREIGTVEEVALPDLPYDDITRTILFAEAASAFEDLIESGVIRGL
                     TAPEDRYTAYARDAILAKDYLKALRLRGVMAREADRVLAPLHALVAPGRASEAALLAS
                     EVRSAIRGTARDVMGAVGNGAGLPAVAVPNGLGARGLPTSLQFMGRAWEEPTVLAAAC
                     AYQSRTDWHERHPADLS"
     gene            complement(1928..2158)
                     /locus_tag="VNU03_13215"
     CDS             complement(1928..2158)
                     /locus_tag="VNU03_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75475.1"
                     /translation="MSEPETIRAPEAIRPEVETMLALVRERYGIRLAPEELEGVRTAI
                     EGIVQAAHALRAVRLTNADEPGQPFAAYRADP"
     gene            complement(2171..3076)
                     /locus_tag="VNU03_13220"
     CDS             complement(2171..3076)
                     /locus_tag="VNU03_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75476.1"
                     /translation="MSSAIRVSGAGTTLAPGAVARGRSSVAEAARMFRRSGSALFGLV
                     LVVVLGLAAALAPLLAPHDPNLIDTARRLARPLTAGHVLGTDEFGRDLLSRLLYGART
                     SLVVGLAATALAAVAGSAFGLLAGFVGRWVDQLVMRSIDTLMAFPYFLLAIAIIATLG
                     PGLVNGMVAVAIVNIPFYGRIVRATVLAVKQTEYVEAARALGVSELRLSLTHVLRNCL
                     APVIVAVTLNVGGMITALAGLSFLGLGVQPPTSDWGSMLSSSRQYMNVAPHVAALPGL
                     AIFLAVLGFNLLGDGLRDALDPRLR"
     gene            complement(3069..4040)
                     /locus_tag="VNU03_13225"
     CDS             complement(3069..4040)
                     /locus_tag="VNU03_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75477.1"
                     /translation="MAAVGGGGLQGYLVRRIFLALPVLVGVSLLVFGVMRLAPGDPAA
                     IMLGAQATREDVERLRRDLGLDRPVAVQYFQWMGRVLQGDLGRSIPLGREVLPEVLLR
                     FKATVVLTGGALVIAIALGLTAGIVSATRQYSWLDKLSMAVAVTGVSLPVFWTGIMLI
                     LVFALSLRWFPSAGMVSPYGGGTGDLLWHLVLPAITLGTASAAAVARLTRSSVLEIIR
                     QDYVRAARAKGLAERAVIARHVLKNAFNPILTVVGVQVGYLLGGAILTETVFSWPGLG
                     SMMVRAIQARDYPLVQGGVLLIATTFVVVNLIVDLLYAVFDPRIRYE"
     gene            complement(4028..5584)
                     /locus_tag="VNU03_13230"
     CDS             complement(4028..5584)
                     /locus_tag="VNU03_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75478.1"
                     /translation="MSRRRAWGMGLGLVAVLLVSVAVAAPPKDAMVIGLLAEPVTMDP
                     PQITDLNSTRVIKRMFEGLTAQELGTYKIIPGLAQSWDISKDGLTYTFHLRPNVKFHD
                     GTPLTAEAVKFCFDRQMNEQGPFYATGTYPYVKGFLGNVAGVEVVNPATVQIRLKAPL
                     TPFLQYLAHHSLYIYSPEALKKWGKDVVKHPVGTGPFKLETWEPGVRVVLSRNDQYWG
                     GAPKVRQAIYVPIIEAQARLSAIKTGEIDLTMDVPPDSLQDLRKDPDVVVAETNSSAV
                     WYVALNTRHPALKDKRVRQALNYAVHKDAIIRDILKGTAIVATTPLSPVYGAYHEDKT
                     QRYPYDPEKAKALLKEAGFANGFDVTFYVPESGSGMQSPVEMGTVIQANLAAVGVRAK
                     IQTMEWGAYLKKYLDGPDMAEMSWNPSIGDPDHMMYMLLSSDRFPPAFNAGFYQNPRV
                     DELLRKGRTTIDDKERIPLYREAQRLVMEDAPWIFVDHGKQVIVHRKRVQGFKLHPNF
                     DLVLTQVWLQ"
     gene            5691..6743
                     /locus_tag="VNU03_13235"
     CDS             5691..6743
                     /locus_tag="VNU03_13235"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWP75479.1"
                     /translation="MAKRMLFIGAGAIGSYLGSFLARAGHDVTLVDPWPEQVEMVRRR
                     GISVTGPHDPFEAQPTALHVHELQRLGADFDIAFIAMKAYDTAWATWMALPHLKPEGY
                     VVSAQNCWNDPIVAGIAGADRSVGLIMSKIGVALWKAGEVERGMEKGQGAGHDVFRAG
                     EHDGRITERVTELADIMSVVDGARPTDNLWGERWSKLCANCMGNPVQAMTGLGSKEIA
                     GQARGREITIRIAQESAAVGLKQGYRIPKFNGREAEQWAQAGRPDVYAELDAGLKPKE
                     GSGRNWRASMAQDVIKGRRSEIDFMNGRVVDKGRELGVPTPVSAAVVALMREVDAGTR
                     KPAPEHIELALTRASA"
     gene            complement(6751..7416)
                     /locus_tag="VNU03_13240"
     CDS             complement(6751..7416)
                     /locus_tag="VNU03_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75480.1"
                     /translation="MSRLALTLALAAALGVAACGPPRVEPRTTIVTGGPRVLFEDDLR
                     APRNWPAATGSICKASYGDGGYIVENIAAAAPCLIGPVQPEVFPASVRIEISARQRKG
                     TREGAFGLMFASRGAAENRTFATLGLTANGTYRVASWTGKWAYPVPPTATRSVKTEYG
                     ALNTLAVELRDKSIVSYVNGRPVATAELPTEASGTLALYVDQRGMEVVFSQLRVVELL
                     PLR"
     gene            7522..8571
                     /locus_tag="VNU03_13245"
     CDS             7522..8571
                     /locus_tag="VNU03_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP75481.1"
                     /translation="MRGIVFTGNRQLEIRDFPDPTPGPGEVVLEIKASGMCGSDLKFY
                     RAAGNEAASLGLGGSGKQVIAGHEPCGVIAAVGSGVSETEARVGQRVMNHHYAGCGRC
                     KHCRVGWSQLCPSGITVFGVTGDGAHARYMKVPARTNVPLPDELSFEEGAAVSCGTGT
                     AYGALKRLDVSGRDTLAVFGQGPVGLSATMLGAAMGARVIAVDIAKERLDLARELGAD
                     AVVNSRETDPVKAIKELTHGEGAETTMDCTGLPEPRVAAVKSAATWGRMCFVGEGGAT
                     TFDISSDLIRKQLTLVASWTFSSMGQWECARFVADRKIPLGRLLTDHFRLDQAVDAYK
                     RFDTQTTGKGVFTSF"
     gene            8571..9125
                     /locus_tag="VNU03_13250"
     CDS             8571..9125
                     /locus_tag="VNU03_13250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWP75482.1"
                     /translation="MADLKILGIPGSLRQASFNRYALVAAQSLLPPGASLDIFELEGI
                     PVYNQDLDKQPPARVVELKARIRAADAILFATPEYNYSVPGVLKNAIDWASRPYGDSA
                     WQGKPVAVMSASVGTFGGARAQYHLRQSFVFLNMYPVNQPEVVIPGAAQRFNERGELT
                     DEVARELIRKLLAELAAWTRKLKG"
     gene            9259..>9754
                     /locus_tag="VNU03_13255"
     CDS             9259..>9754
                     /locus_tag="VNU03_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP75483.1"
                     /translation="MAKPAQSKATRRSFLKLAAAGAAGVAAGPWVSRNAQAKPVSITF
                     ARESSYVKNFDVHFQNVMAPAYEKATGIKIEYQIQAAGGSAVPQLVSMVENKNGADLA
                     WTQQEWLYRDALVDVSDIAEEVGKQQGGWYDEIKRLSIDKGKWKSIPNGNIGQLMNYR
                     KDWFD"
BASE COUNT         1562 a         3428 c         3457 g         1307 t
ORIGIN      
        1 cctcgttggt cgcactcatt caaataggag aaccgcaatg aggacaatca ttggatggtc
       61 tgtgggcgcc gcgtgcgcga ccgtgatcgt gaccggcctt tccgcgctcc ccgcacccgc
      121 acaggaaacc aagaccgacc ggcgcgagat ccggcaggac tctcgcgaga tccgcggtga
      181 ccggaaggag atcgccaagg acacgcagga agtccggggg gaccgcaagg acctcgaggc
      241 gtcccgccag aagctgcgcg aggcctacaa gtccggtgac cccgcggcca tcaaggcggc
      301 ccgcgagagc taccagaaga gccggggctc gctccacggt gatgtgaagg atcgacgcca
      361 ggacgctcgc gacctgcgcc aggacaagca ggatcgccgt gaggacgtcc aggatctccg
      421 ccaggaccag cgccaggcgc gcacccgcca ggccaagtaa cccgcgaccg gaactctcaa
      481 gggggagcgg cccatcgggc cctccccctt tcgtttgccc gcggctagct cagatcggcc
      541 ggatggcgct cgtgccagtc ggtccgcgac tggtaggcgc acgccgcggc caggacggtc
      601 ggctcctccc acgcccgccc catgaactgc aggctggtgg gcagcccgcg cgcgccgagc
      661 ccgttcggca ccgccaccgc gggcagcccc gcgccgttgc ccaccgcgcc catcacgtcg
      721 cgcgcagtcc cgcgaatggc gctgcgcacc tcgctcgcga ggagagccgc ctccgacgcg
      781 cggccggggg ccaccagcgc gtggagcggg gcgagcacgc ggtccgcctc gcgcgccatc
      841 accccgcgca gccgtagcgc cttcaggtaa tccttggcca ggatcgcgtc gcgcgcgtag
      901 gcggtgtagc ggtcctccgg cgcggtcagg ccgcggatca cgccgctctc gatgaggtcc
      961 tcgaaggcgc tggccgcctc cgcgaagagg atcgtccggg tgatgtcgtc gtagggcagg
     1021 tccggcagcg cgacctcctc cacggtgccg atctcccgca gcacgccgag cgaccgctcg
     1081 aagttctcgc gcaccgcctc ctcgcaaccg tcggtggccc cgcggagcac cccgaaccgg
     1141 aattgccgcc cggccggggc cgcgggctcg tggcggaacg ggcggtcgac ggtggtcggg
     1201 tcggccggat cgcgacccgc gatcgcctcg agcaccaggc cgcagtcgtc ggcggtgagg
     1261 gcgagcgggc ccagcttgtc gagcgtccag cagagcgcca tcgccccgtg acggctcacc
     1321 cggccgtagg tcggccgcag gcccgcgacc ccgcagttgc ccgccggcga gaggatcgag
     1381 ccccacgtct ccgagccgat cgcgaacggc acgaggcccg ccgcgaccgc cgagcccgag
     1441 ccgctcgacg atccgccact ccacgcgtcg cggctccacg ggttgacgcc ggggccggtg
     1501 aaggccgcgt tcggctggcg atagcccatg ccgccggcca gctccaccat cgcgagcttg
     1561 gcgcagagca ccgcgcccgc cgcctcgagc ttgcggatcg cggtggcgtc gaagtcgaac
     1621 acgcgggcct tgaacggcgc ggcgccccac gtggtgggga tgcctcccga ggtggcgagc
     1681 aggtccttgg ccccgtacgg gatgccgtgc agcggtccgc ggtagcggcc ggccgcgatc
     1741 tccgcctccg cgcggcgggc ctgctcgagc gcgcgggccc gggtgaccgt caccaccgcg
     1801 ttgtagcgcg ggcccaggcg ctccagccga tcgaggaagg tctcggccag cgccaccggg
     1861 gagagccggc gctcccggat caggtcgccg agccggcgca ccgggacgaa gacgacctct
     1921 tcggccatca cggatccgcc cggtaggccg cgaagggctg gcccggctcg tcggcgttgg
     1981 tgagccgcac cgcgcggagg gcgtgcgcgg cctggacgat cccctcgatg gcggtgcgca
     2041 caccctcgag ctcctccggg gcgaggcgga tgccgtagcg ctcgcgcacg agcgcgagca
     2101 tggtctcgac ctcagggcga atcgcctcgg gcgcgcggat cgtctcgggc tcgctcatcg
     2161 ccccggcatt ctagcggagg cgcgggtcga gcgcatcccg gagcccgtcg cccagcaggt
     2221 tgaagccgag caccgcgagg aagatcgcca gccccggcag ggccgccacg tggggcgcca
     2281 cgttcatgta ctgccggctg ctggagagca tgctgcccca gtcggaagtc ggcggctgga
     2341 cgcccaggcc caggaacgag agcccggcca gcgcggtaat catgccgccc acgttcaggg
     2401 tcaccgccac gatgaccggg gccaggcagt tgcgcaggac gtgggtgagc gacaggcgca
     2461 gctcggagac gccgagggcc cgcgccgcct ccacgtactc ggtctgcttc accgccagca
     2521 cggtggcgcg cacgatgcgc ccgtagaacg ggatgttcac gatcgcgacc gccaccatgc
     2581 cgttgacgag gcccgggccg agggtggcga tgatcgcgat ggcgagcagg aaatagggga
     2641 acgccatcag ggtgtcgatc gagcgcatga cgagctgatc cacccagcgg cccacgaagc
     2701 cggccagcaa tccaaacgcc gaccccgcca ccgcggcgag cgcggtggcg gcgagcccca
     2761 ccaccagcga ggtgcgggca ccgtagagca accggctcag cagatcgcgg ccgaactcgt
     2821 cggtgccgag cacgtgcccg gcggtgagcg ggcgcgctag gcgccgcgcg gtgtcgatga
     2881 gattgggatc gtgcggagcc agcagcggcg ccagcgccgc ggcgaggccg agcaccacca
     2941 ccagcacgag cccgaacaac gcgctcccgc tgcggcggaa catgcgggcg gcctcggcca
     3001 cgctcgaccg gccccgcgcc accgcgcccg gcgccagggt ggtgcccgcg ccgctcaccc
     3061 ggatcgcgct actcatagcg gatgcgcgga tcgaagaccg cgtagagcag atcgacgatc
     3121 aggttcacca ccacgaaggt ggtggcgatc agcagcaccc cgccctgcac cagcggatag
     3181 tcgcgcgcct ggatcgcccg caccatcatc gagccgagcc cgggccacga gaagacggtc
     3241 tcggtcagga tcgcgccgcc gaggaggtag cccacctgca caccgaccac ggtgaggatg
     3301 ggattgaacg cgttcttcag cacgtggcgg gcgatcaccg cgcgctcggc caggcccttg
     3361 gcccgcgcgg cccgcacgta gtcctggcgg atgatctcga gcacgctcga gcgggtgagc
     3421 cgcgccaccg cggccgccga cgcggtcccg agggtgatcg cgggcagcac cagatgccag
     3481 aggaggtcgc cggtcccgcc cccgtacggc gacaccatgc cggcagaagg aaaccagcga
     3541 agcgacaagg caaacaccag gatcagcatg atgccggtcc agaacaccgg caggctcacc
     3601 ccggtcaccg ccaccgccat gctgagcttg tcgagccagg agtattgccg cgtggcggac
     3661 acgatgccgg cggtgaggcc gagggcgatc gcgatcacca gcgcgccgcc ggtcaggacc
     3721 acggtcgcct tgaagcgcag cagcacctcg ggcagcacct cgcgcccgag cgggatggag
     3781 cggccgagat cgccctggag cacccggccc atccactgga agtactgcac cgccaccggc
     3841 cggtcgagcc cgaggtcgcg gcgcaggcgc tccacgtcct cgcgggtggc ctgcgcgccc
     3901 agcatgatcg cggcggggtc gccgggggcc aggcgcatca ccccgaagac gagcagcgag
     3961 accccgacca ggaccggcag cgcgaggaag atgcgtcgga cgaggtagcc ctggaggccg
     4021 cccccgccta ctgcagccac acctgcgtca gcaccagatc gaagttgggg tggagcttga
     4081 acccctgcac gcgcttcctg tgcacgatca cctgcttgcc gtggtcgacg aagatccacg
     4141 gcgcgtcctc catcacgaga cgctgggcct cccggtagag cgggatgcgc tccttgtcgt
     4201 cgatggtggt gcggcccttc cggagcagct cgtcgacgcg ggggttctgg tagaagcccg
     4261 cgttgaacgc gggcgggaag cggtcggagg agagcagcat gtacatcatg tggtcgggat
     4321 cgccgatgga cgggttccag gacatctcgg ccatgtcggg gccgtcgagg tacttcttga
     4381 ggtaggcgcc ccactccatg gtctggatct tggcgcgcac gcccaccgcg gccaggttgg
     4441 cctggatcac ggtgcccatc tcgaccggcg actgcatgcc ggagcccgac tcgggcacgt
     4501 agaacgtcac gtcgaacccg ttggcgaagc cggcctcctt gaggagcgcc ttggccttct
     4561 ccgggtcgta gggataccgc tgcgtcttgt cctcgtggta cgcgccgtac accggcgaga
     4621 gcggggtggt ggccacgatc gcggtgccct tcaggatgtc gcggatgatc gcgtccttgt
     4681 gcaccgcgta gttgagcgcc tggcgcaccc gcttgtcctt cagcgcggga tggcgggtgt
     4741 tcagtgccac gtaccagacc gcggaggagt tggtctcggc caccaccacg tcgggatctt
     4801 ttctgagatc ctggaggcta tccggcggca cgtccatggt gagatcgatc tcgccggtct
     4861 tgatcgccga gaggcgcgcc tgcgcctcga tgatcggcac gtagatcgcc tggcggacct
     4921 tcggcgcgcc gccccagtac tgatcgttgc gcgagagaac gacccgcacc cccggctccc
     4981 acgtctcgag cttgaagggc ccggtgccca ccgggtgctt caccacgtcc ttgccccact
     5041 tcttgagcgc ctcggggctg tagatgtaga gcgagtggtg cgcgaggtac tggagaaacg
     5101 gggtcagcgg cgccttgagc cggatctgca cagtggccgg attcaccacc tccacccccg
     5161 ccacgttgcc gaggaagccc ttcacgtacg ggtaggtgcc ggtcgcgtag aacggcccct
     5221 gctcgttcat ctggcggtcg aagcagaact tcaccgcctc cgcagtcagc ggggtgccgt
     5281 cgtggaactt cacgttgggg cggaggtgga aggtgtaggt caggccgtcc ttggagatgt
     5341 cccaggactg ggcgaggccc gggatgatct tgtaggtccc cagctcctgg gcggtgagcc
     5401 cctcgaacat gcgcttgatg acgcgcgtcg agttcaggtc ggtgatctgg ggcgggtcca
     5461 tggtcaccgg ctccgcgagg aggccgatca ccatcgcgtc cttcggcggc gccgcgaccg
     5521 cgacggatac cagcagcacg gccaccagcc ccaaccccat tccccacgcg cggcgtctcg
     5581 acatgcgggc ctcctccggg atagtgaaat gctccgcatc ttgccccaac cacctcggct
     5641 tggcaacggc ccggcgcggc gctaagatcc cggcctggga ggacctccag atggccaagc
     5701 gaatgctctt catcggcgcg ggcgcgatcg gcagttacct gggctcgttc ctcgcgcggg
     5761 ccggccacga cgtgaccctc gtggacccgt ggcccgagca ggtcgagatg gtgcgccgcc
     5821 gcgggatctc ggtgaccggc ccgcacgatc ccttcgaggc gcagcccacc gcgctgcacg
     5881 tgcacgagct ccagcgcctc ggcgccgact tcgacatcgc gttcatcgcg atgaaggcct
     5941 acgacaccgc gtgggccacc tggatggccc tgccccacct gaagccggag ggctacgtgg
     6001 tctcggccca gaactgctgg aacgatccga tcgtcgccgg gatcgcggga gcggaccgct
     6061 cggtcggtct catcatgtcc aagatcgggg tcgcgctctg gaaggcgggc gaggtggagc
     6121 gcggcatgga gaagggccag ggcgccggcc acgacgtctt ccgcgcgggc gagcacgacg
     6181 ggcgcatcac cgagcgggtc accgagctgg ccgacatcat gagcgtggtc gacggcgcgc
     6241 ggcccaccga caacctgtgg ggcgagcgct ggtccaagct ctgcgccaac tgcatgggca
     6301 acccggtgca ggccatgacc gggctcggca gcaaggagat cgcggggcag gcgcgcgggc
     6361 gcgagatcac catccgcatc gcgcaggagt ccgccgcggt cggcctcaag cagggctacc
     6421 gcatccccaa gttcaacggc cgcgaggccg agcagtgggc ccaggccggc cggcccgacg
     6481 tctacgccga gctcgacgcc gggctcaagc ccaaggaagg gtcgggccgc aactggcgcg
     6541 cgtcgatggc ccaggacgtg atcaagggcc gccggagcga gatcgacttc atgaacggcc
     6601 gcgtggtcga caagggccgc gagctgggcg tgccgacccc ggtctcggcc gcggtggtgg
     6661 cgctgatgcg cgaggtcgac gcgggcaccc gcaagcccgc ccccgagcac atcgagctgg
     6721 cgctcacccg cgcgagcgcc tgaccgggga ttaacgcagc ggcagcagct cgaccacccg
     6781 gagctggctg aacaccacct ccatgccccg ctggtccacg tagagcgcga gcgtgccgga
     6841 ggcctcggtg ggcagctcgg cggtggccac cggacgcccg ttgacgtacg acacgatgct
     6901 cttgtcgcgc agctctaccg ccagcgtgtt cagcgcgccg tactcggtct tgacgctgcg
     6961 ggtggcggtg ggcggcaccg ggtaggccca tttgccggtc cagctcgcca cccggtaggt
     7021 cccgttggcg gtgagcccca gggtcgcgaa ggtgcgattc tcggcggccc cgcgactcgc
     7081 aaacatgagg ccgaaggcgc cctcgcgcgt gcccttccgc tggcgggccg agatctcgat
     7141 gcgcaccgag gccgggaaga cctcgggctg caccggaccg atcaggcacg gggcggcggc
     7201 cgcgatgttc tccacgatgt agccgccgtc gccgtaggag gccttgcaga tggagccggt
     7261 ggcggccggc cagttgcggg gcgcgcgcag atcgtcctcg aagagcacgc ggggcccgcc
     7321 ggtcacgatg gtggtgcgcg gctcgacccg cgggggcccg cacgccgcga cgccgagcgc
     7381 cgccgccagc gcgagggtca gagcgagccg ggacatcgaa tgcctcccgt gatggtgagc
     7441 ggtgcggaca tctggcccag ggccatcaca tcttttacac tacaccgggt tcgaacgaca
     7501 acggcagtcg gggaggcgtc catgcgaggc atcgtgttca cgggaaatcg ccagctcgag
     7561 atccgggact tccccgaccc cactcccggc cccggagagg tcgtgctcga gatcaaggcc
     7621 tccggcatgt gcgggagcga tctgaagttc tatcgcgcgg cgggcaacga ggccgcctcc
     7681 ctcggcctgg gcggcagcgg caagcaggtc atcgcggggc acgagccctg cggggtgatc
     7741 gcggcggtgg ggtccggggt cagcgagacc gaggcccgcg tgggccagcg ggtcatgaac
     7801 caccactacg ccggctgcgg gcgctgcaag cactgccggg tcggctggtc gcagctctgc
     7861 ccgagcggca tcaccgtgtt cggcgtcacc ggtgacggcg cccacgcgcg ctacatgaag
     7921 gtgccggccc gcaccaacgt gccgctaccg gacgagctca gcttcgagga gggcgccgcc
     7981 gtctcctgcg ggaccggcac cgcctacggc gcgctgaagc gtctggacgt ctcggggcgc
     8041 gacaccctcg ccgtgttcgg ccaggggccg gtcggcctct ccgccaccat gctgggcgcc
     8101 gcgatggggg cacgggtgat cgcggtggac atcgcgaagg agcggctcga cctggcccgg
     8161 gagctgggcg ccgacgcggt cgtgaactcg cgcgagaccg atccggtgaa ggccatcaag
     8221 gagctgaccc acggggaagg cgcggagacc accatggact gcaccgggct ccccgagccg
     8281 cgggtggccg cggtgaagag cgcagccacc tggggccgca tgtgcttcgt gggcgagggc
     8341 ggagccacca ccttcgacat cagcagcgac ctgatccgca agcagctcac cctcgtggcc
     8401 tcgtggacct tcagcagcat gggccagtgg gagtgcgcgc ggttcgtggc ggaccggaag
     8461 atcccgctgg gccggctcct caccgatcac ttccgcctcg accaggcggt ggatgcctac
     8521 aagcgcttcg acacccagac gaccggcaag ggcgtcttca cgagcttctg atggccgatc
     8581 tcaagatcct cggcatcccc ggtagcctgc gccaggcgtc cttcaaccgg tacgcgctgg
     8641 tggcggccca gtcgctgctg ccgcccggcg cgtcgctcga catcttcgag ctggagggca
     8701 tcccggtcta caaccaggac ctcgacaagc agccgcccgc ccgggtggtg gagctgaagg
     8761 cgcggatccg cgccgccgac gccatcctgt tcgccacccc cgagtacaac tactcggtgc
     8821 ccggcgtgct gaagaacgcg atcgactggg cttcgcggcc ctacggcgac agcgcgtggc
     8881 agggcaagcc ggtcgcggtg atgagcgcct cggtgggcac cttcggcggc gcgcgcgccc
     8941 agtaccacct gcgccagtcc ttcgtgttcc tcaacatgta tccggtgaat cagccggaag
     9001 tggtgatccc cggcgcggcg cagcgcttca acgagcgcgg cgagttgacc gacgaggtgg
     9061 cgcgcgagct gatccgcaag ctgctcgcgg agctggcggc ctggacgaga aagctgaagg
     9121 gataggcccc tcaccctccc ccccgagggg agagggggat ccccgggccc cgtggcaccg
     9181 ctcaggcggc ccttgacgat gccccggtcc cggggcagga tctgggccat ccaggtcact
     9241 cccaaagggg gaaactgcat ggcaaagccc gctcagtcga aggccacccg tcgtagcttt
     9301 ctgaagctcg ccgccgccgg ggccgccggc gtcgcggcgg gcccgtgggt ttcccgcaat
     9361 gcccaggcca agccggtctc catcacgttc gcgcgcgaaa gctcctacgt gaagaacttc
     9421 gacgtgcact tccagaacgt gatggcaccc gcctacgaga aggccaccgg catcaagatc
     9481 gagtaccaga tccaggccgc gggcggcagc gcggtgcccc agctggtctc gatggtcgag
     9541 aacaagaatg gcgcggacct ggcatggact cagcaggagt ggctctaccg cgacgccctc
     9601 gtagacgtgt ccgacatcgc cgaggaggtg ggcaagcagc agggcggctg gtacgacgag
     9661 atcaagcgcc tgtcgatcga caagggcaag tggaagtcca tccctaacgg gaatatcggc
     9721 cagctgatga actaccggaa ggactggttc gacg
//
LOCUS       DATMCV010000574         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_432859, whole genome shotgun sequence.
ACCESSION   DATMCV010000574 DATMCV010000000
VERSION     DATMCV010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432859"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..586)
                     /gene="bamD"
                     /locus_tag="VNU03_13260"
     CDS             complement(116..586)
                     /gene="bamD"
                     /locus_tag="VNU03_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWP75484.1"
                     /translation="MTRGLRALPILLLLVACSTARPAPWAGSPPGRGGPGAATRMVGY
                     ADDLVERGEVQEAFRVYERVLRDYPADPASASALYGLGRLQAAPGSQLRSYRAAHAAF
                     SRLLSEYPQSRWESEARAWRAVLGDLLAREDETARVKTQLERLRRTDLDLERRH"
     gene            complement(583..2028)
                     /locus_tag="VNU03_13265"
     CDS             complement(583..2028)
                     /locus_tag="VNU03_13265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWP75485.1"
                     /translation="MRLGPKIFLVSALAIVALSSSVGWSLLTVKRLVSVNQEIATRSV
                     PALRLQGALRETLHGLVRLETRALVLADRDYARAWSERAARMTGELDEVARYLETPEE
                     RAAHAAVRAALDDYRAHVEEERRLVASARPAAALRLAEGPARDATQRVEAALAAMTSA
                     TETALAHTQARARELEAWTWHAVAVALLISLAVALAASAWLAFRMTRSLRRLSAATAA
                     LASGTWTGPLAVPDRDEIGELSRSFDLMADRLRQVDHLKEEFFSHVSHDLRNPLSAIR
                     LAAETLQERARTTADPRGLRLAQIIDASAGRMLGMVNQILDFTRLRALAVSLDTRPVD
                     ALGAVTRAMDELRPVAEAKRIRLTLAAEGNDFIVLGEEGSLVRVVINLLGNAVNFTAP
                     DGTVTLRLAEAGDHLELQVRDTGVGIPPEALGSIFEPYRQAHGRREGSGLGLAVVKGL
                     VEAHHGTVSVDSAPGRGSCFTIELPKAKAAA"
     gene            complement(2057..>2459)
                     /locus_tag="VNU03_13270"
     CDS             complement(2057..>2459)
                     /locus_tag="VNU03_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75486.1"
                     /translation="MSEARAARRILVVDDDDALREVLTVALTRDGHTVDGACDGTEAL
                     ALLDRQPYDLVLSDLRMPRVDGPSLYETLRNRHHFPVRFATKLPRVIFMTGNAAEHAD
                     FLRGTTDPILEKPFPLRVVRQMVSVLLNASA"
BASE COUNT          323 a          853 c          959 g          324 t
ORIGIN      
        1 agcggggtcg cgacgcgatg gggctcgccg ggatcgcggg gccgcattcc acccgacggc
       61 gtcatggcaa gcgagagcgt agcacgcacc gctcccgcgt ccggcccacc gcggtctagt
      121 gccgacgctc gaggtccaga tccgtgcggc gcagtcgctc gagctgggtc ttcacccgag
      181 cggtctcgtc ctcgcgcgcg agcagatcgc cgagcaccgc ccgccaggcg cgcgcctcgc
      241 tctcccagcg gctctgcggg tactccgaca ggaggcgcga gaaggcggcg tgggccgcgc
      301 ggtagctgcg gagctggctt cccggggccg cctgcaggcg cccgagcccg tagagggcgg
      361 aggcggaggc cgggtccgcc gggtagtccc gcagcacgcg ctcgtagacg cggaaggcct
      421 cctggacctc gccgcgctcg acgaggtcgt ccgcgtaccc gaccatccgc gtcgcggctc
      481 cgggtccgcc gcgcccggga gggctgcccg cccacggggc ggggcgcgcg gtgctgcagg
      541 cgaccagcag caagaggatg ggaagcgcgc gcaggccgcg ggtcatgccg cggctttcgc
      601 cttcggcagc tcgatggtga agcagctgcc ccggcccggc gccgagtcga cgctcaccgt
      661 gccgtggtgg gcctcgacca ggcccttgac caccgccagg ccgaggccgc tgccctcgcg
      721 ccgcccgtgg gcctgacggt acggctcgaa gatcgacccg agggcttccg gcgggatgcc
      781 gacgccggtg tcgcgcacct gcagctcgag gtggtcgccg gcctcggcga ggcgcagcgt
      841 caccgtcccg tccggggccg tgaagttcac cgcgttgccc aggaggttga tgacgacgcg
      901 caccaggctg ccttcctccc cgagcacgat gaagtcgttg ccttccgcgg ccagggtcag
      961 ccggattcgc ttggcttccg cgacgggtcg cagctcgtcc atggcgcgcg tgaccgcgcc
     1021 gagcgcgtcg accggccggg tgtcgagcga caccgcgagg gcccgcagcc gagtgaagtc
     1081 gaggatctgg ttgaccatcc cgagcatccg cccggcgctc gcgtcgatga tctgggcgag
     1141 cctcaggccc cggggatcgg ccgtcgtccg ggcccgctcc tgcagggtct cggcggcgag
     1201 gcggatcgcg gagaggggat tgcgcaggtc gtgggagacg tgcgagaaga actcctcctt
     1261 gaggtggtcg acctgacgca ggcgatccgc catcagatcg aaggaccggc tcagctcgcc
     1321 gatctcgtcg cgatcgggca ccgccagcgg accggtccac gttccgctcg cgagcgcggc
     1381 ggtggcggcg gacaggcgcc tcagcgagcg cgtcatccgg aacgcgagcc acgcgctggc
     1441 cgcgagagcc acggccaggc tgatcaggag cgcgacggcc accgcgtgcc acgtccacgc
     1501 ctcgagctca cgggcccggg cctgcgtgtg ggcgagcgcg gtctcggtgg ccgaggtcat
     1561 ggccgcgagc gcggcctcca cccgctgggt ggcgtcccgc gcggggccct ccgcgaggcg
     1621 tagcgcggcg gcggggcggg cggaggcgac cagccgccgc tcctcctcga cgtgggcgcg
     1681 atagtcgtcg agggccgccc gcaccgccgc gtgggcggcg cgctcctccg gtgtctcgag
     1741 gtaccgggcg acctcgtcga gctcgccggt catgcgggcg gcccgctcgc tccacgcccg
     1801 cgcgtagtcc cggtccgcga gcaccagggc gcgcgtctcg aggcgcacca ggccgtgcag
     1861 cgtttccctc agcgcgccct gcaagcgtag cgccggtacc gaccgggtgg cgatctcctg
     1921 gttgaccgag acgaggcgct tgaccgtgag gaggctccag ccgaccgagc tggagagtgc
     1981 gacgatggcg agggcggaga cgaggaagat cttggggccg agtcgcatgg gtcggggtgg
     2041 cagcggccgg gcggacttac gcgctggcgt tgagtagcac gctcaccatc tgccgtacga
     2101 cccgcagcgg gaacggcttc tcgaggatgg gatcggtggt gccgcggagg aagtcggcgt
     2161 gctccgcggc gttgccggtc atgaagatca cccggggcag cttggtggcg aagcggaccg
     2221 ggaagtgatg gcgattgcgg agggtctcgt agagggacgg cccgtccacg cggggcatcc
     2281 ggagatcgct gaggacgaga tcgtagggct ggcggtccag cagggccagg gcctcggtgc
     2341 cgtcgcaggc gccgtcgacg gtgtgcccgt cacgggtcag ggcgacggtg aggacctccc
     2401 ggagggcgtc gtcgtcgtcg acgacgagga tacggcgggc cgcgcgcgct tctgacatg
//
LOCUS       DATMCV010000575         4983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_562547, whole genome shotgun sequence.
ACCESSION   DATMCV010000575 DATMCV010000000
VERSION     DATMCV010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562547"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VNU03_13275"
     CDS             complement(<1..1407)
                     /locus_tag="VNU03_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HWP75487.1"
                     /translation="MEAIWPLPRRARIALVWVLLVVSVCALGLAPAALAQTPTPPTAA
                     VAAPHQGGGEASLKLPDLSQATFVGMNGRTMLQWGLLVCLLGLAFGLVIYSQLKNLPV
                     HQSMKDISELIYETCKTYLITQGKFLAILELFIAVIIVFYFGFLQHFEGIKVFIILLF
                     SVVGILGSYGVAWFGIRINTFANSRTAFASLAGKPFPVYAIPIKAGMSIGMLLVSVEL
                     FIMLCILLYIPRDYAGPCFIGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIVFNIK
                     EDDARNPGVIADCTGDNAGDSVGPTADGFETYGVTGVALITFILLAVPKELVQVQLLV
                     WIFVMRVIMIVASGASYLVNEAFAKARYGTAAKMSFEAPLTSLVWVTSIVSVVLTYAA
                     SWLMIPELGDGSLWWKLSTVITCGTLAGAIIPEFVKVFTSTNSAHVREVVTSAREGGA
                     SLGILSGLVAGNFSAYWLG"
     gene            complement(1544..1783)
                     /locus_tag="VNU03_13280"
     CDS             complement(1544..1783)
                     /locus_tag="VNU03_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWP75488.1"
                     /translation="MKKALVFTMEGNADGEAALAFFQARGVAVDVRDVGRDPQASLDL
                     FARAGRLGVPTVVIGDRMFDGFGTHREEIEALLRD"
     gene            1858..2130
                     /locus_tag="VNU03_13285"
     CDS             1858..2130
                     /locus_tag="VNU03_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWP75489.1"
                     /translation="MSRIDLVDTMAAASGWTKAGADRALRAFLASVRGSLKRGQPVTL
                     VGFGTFSVSRRKPRKGKNPRTGQPMHVAGGRTPRFKPSKKLKRAVR"
     gene            2178..3104
                     /locus_tag="VNU03_13290"
     CDS             2178..3104
                     /locus_tag="VNU03_13290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWP75490.1"
                     /translation="MRVIPALIVSVSLSLIAAPALAQTSSQNSRPPALSAEAVLLLDT
                     QGRVVFAKNAAESHAPASLVKLMTLYLACEALEAGRAQWEEPVVVSHRVVLTPRYRMG
                     LRTGEAVPLRTLLEGVAIASANDAAAAVAEHLGGTEEAFVAAMNAKAEELGLTHTHYA
                     NPHGLPDPNQRTTAEDLAKLVGRLLDDYPASRPLLGGATFVYRGRVYSRNIPLFRDPG
                     GVQALKTGFTREAGYNLAISAWKSGQQFVLITLGSQTRSLSFRDAKAILRYGFYETGL
                     EAAPPPAAPRRPAKPPRRPAQRGSRAALSGAG"
     gene            3106..3771
                     /locus_tag="VNU03_13295"
     CDS             3106..3771
                     /locus_tag="VNU03_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HWP75491.1"
                     /translation="MAAPRAFVFDAYGTLFDVHSVVEAARALTSDPEALSLLWRQKQL
                     EYTWLRSLMGRYEDFWLVTGQALRFALRRLGVAAGQSQIESLMEAYLALSPFPEVPAA
                     LRALRGTPLAILSNGSPRMLESAVRSSGLAGAFDHVLSVDAVGVYKPSARVYEMAPRA
                     LGLSAGEIVFVSSNGWDVAGAAAYGFTTCWCNRANAPAEELDLTPDYEVDRLDAIPEK
                     LGL"
     gene            complement(3833..3991)
                     /locus_tag="VNU03_13300"
     CDS             complement(3833..3991)
                     /locus_tag="VNU03_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75492.1"
                     /translation="MAKKKLAFGGYAINFKGCDDTLESVMGSKPIGPSEMTKKIWAYV
                     KRKRLAKK"
     gene            complement(4173..>4983)
                     /locus_tag="VNU03_13305"
     CDS             complement(4173..>4983)
                     /locus_tag="VNU03_13305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75493.1"
                     /translation="MADIVTDGRVIMGVGRGYHTREVESFGAPLLDAAANRELFEEQM
                     EVLLKCFNEESWSHRGKHYVIPPDVDYRGYRLREVTCVPRPIHRPVEIWMPIASGKTI
                     DYMARMGLKAMVTLNGEKVLDQVARAYRDACVRHGRPKELGEDLCWGVGLYLADSREE
                     AIRRLEPAHDERYKWFAPFGFVRYADEQGRSWGTPGAPARVPTLREGVEQKAWLVGRP
                     AEVIETIREIEARYPGLDQCMIHWAEGIPPAEFKEQLRWFARDVMPAFVGR"
BASE COUNT          773 a         1762 c         1695 g          753 t
ORIGIN      
        1 accgagccag taggcgctga agttgcccgc caccaggccg gagaggatgc cgagcgaggc
       61 tccgccctcg cgcgcggagg tgaccacctc gcgcacgtgg gccgagttgg tggaggtgaa
      121 caccttgacg aactcgggaa tgatcgcgcc ggccagggtg ccgcaggtga tgaccgtcga
      181 gagcttccac cacaggctgc cgtcgccgag ctccgggatc atgagccagg acgcggcgta
      241 ggtcagcacc accgacacga tcgaggtgac ccacaccagg ctggtcagcg gggcctcgaa
      301 gctcatcttg gccgcggtgc cgtagcgggc cttcgcgaag gcctcgttga cgaggtagga
      361 ggcgccgctc gccacgatca tgatcacgcg catcacgaag atccacacca gcagctgcac
      421 ctgcaccagc tccttgggca ccgcgagcag gatgaaggtg atcagggcca ccccggtgac
      481 cccgtaggtc tcgaacccgt ccgcggtggg gccgaccgag tcgcccgcgt tgtcgccggt
      541 gcagtccgcg atgacgcccg ggttgcgcgc gtcgtcctcc ttgatgttga acacgatctt
      601 catcaggtcg gagccgatgt cggcgatctt ggtgaagata ccgcccgcga tgcggagggc
      661 ggccgccccg agcgactcgc cgatggcgaa gccgatgaag cagggccccg cgtagtcgcg
      721 cgggatgtag agcaggatgc acagcatgat gaacagctcg accgacacca gcagcatccc
      781 gatgctcatc cccgccttga tcgggatcgc gtagaccggg aacggcttgc ccgcgaggct
      841 cgcgaaggcg gtgcgggagt tggcgaaggt gttgatgcgg atgccgaacc acgccacccc
      901 gtaggagccc aggatgccga ccacgctgaa gagcaggatg atgaagacct tgatcccctc
      961 gaagtgctgc aggaacccga agtagaagac gatgatcacc gcgatgaaca gctcgaggat
     1021 cgcgaggaac ttgccctggg tgatcaggta ggtcttgcag gtctcgtaga tcagctccga
     1081 gatgtccttc atcgactggt gcaccggcag gttcttcagc tgcgagtaga tcaccaggcc
     1141 gaaggccagg cccaggaggc agaccagcag accccactgc agcatggtgc gcccgttcat
     1201 gcccacgaag gtcgcctggg acaggtcggg gagcttgagc gacgcctcgc ccccaccctg
     1261 gtgcggcgcg gccacagcgg ccgtgggcgg ggtgggggtc tgagccagcg cggccggggc
     1321 taggcccagc gcgcacacgc tcacgacgag cagcacccag acgagcgcga tgcgcgcgcg
     1381 ccggggcaag ggccagatcg cttccatagt ccgaatctcc tcgttgagcg agtttcgaag
     1441 ggcgggtcac ggcggcgcgg tcggccaccg gcccaacgcg tgtttatacc gcaggcgccc
     1501 gcctccgtca acgcggcgcg ttgcgccgcg cgggcgggcc cgcctagtcg cgcaggaggg
     1561 cctcgatctc ctcccggtgc gtgccgaagc cgtcgaacat gcggtcgccg atcacgacgg
     1621 tgggcacacc caggcggccc gcgcgggcga acaggtcgag gctcgcctgc gggtcgcggc
     1681 ccacgtcgcg cacgtccacc gccacgcccc gcgcctggaa gaacgccagt gcggcctcgc
     1741 cgtccgcatt gccttccatc gtgaagacga gcgctttttt caccggggcc acgataccac
     1801 ctcgaccttg acgctccaac gtcccgtttg ctagcatccg cgaccggtcg gagagacgtg
     1861 agccggatcg atctcgtgga cacgatggcc gccgccagcg gctggaccaa ggccggcgcc
     1921 gaccgcgcct tgcgcgcgtt cctcgcctcg gtgcgcggct cgctcaagcg cggccagccg
     1981 gtcacgctgg tcggcttcgg caccttctcg gtgagccgac gcaagccccg caaggggaag
     2041 aacccgcgca ccggacagcc gatgcacgtg gcgggcggac gcaccccacg cttcaagccg
     2101 tcgaagaagc tcaagcgcgc ggtgcgctag ggattttctc tgctcggcta attctggccg
     2161 cgggtaagat aggggcagtg cgcgtcatcc cggccctcat cgtcagcgtg tccctctcgc
     2221 tcatcgcagc cccggctcta gcccagacca gcagtcagaa cagccggccg cctgccctga
     2281 gcgcggaagc ggtgctcctc ctcgacacgc agggtcgcgt ggtcttcgcc aagaacgcgg
     2341 ccgagtccca cgcgcccgcg agcctcgtca agctcatgac cctctacctc gcctgcgagg
     2401 cgctcgaggc ggggcgggcc cagtgggagg agccggtggt ggtgagccat cgcgtggtcc
     2461 tgacgccccg ctaccgcatg ggcctccgga ccggcgaggc ggtgcccctg cgcaccctgc
     2521 tcgagggcgt ggccatcgcc tccgccaacg acgccgccgc cgcggtggcc gagcacctcg
     2581 gcggcaccga ggaggccttc gtggccgcga tgaacgccaa ggccgaggag ctgggcctga
     2641 cccacacgca ctacgccaac ccgcacggcc tgcccgatcc caaccagcgc accaccgccg
     2701 aggacctcgc caagctcgtc ggccggctcc tcgacgacta tcccgcctcg cgtccgctcc
     2761 tcgggggggc gacgttcgtg taccgcgggc gggtctatag ccggaacatc ccgctgttcc
     2821 gcgatccggg tggcgtgcag gcgctcaaga ccggcttcac ccgcgaggcg ggctacaacc
     2881 tcgcgatctc cgcctggaag tcgggccagc agttcgtcct catcacgctc ggctcccaga
     2941 cccgcagcct ctccttccgc gacgcgaagg ccatcctccg ctacggcttc tacgagaccg
     3001 gcctggaggc cgcgcccccg ccggcggcgc cgcgtcgacc cgcgaagcct ccccggcgcc
     3061 cggctcagcg cggctcgcgg gcggccctgt ccggggccgg ctagggtggc cgcgccccgc
     3121 gccttcgtct tcgacgccta cggaacgctg ttcgacgtcc actcggtcgt ggaggcggcg
     3181 cgcgcgctga cctcggatcc ggaggcgctg tccctgctct ggcgtcagaa gcagctcgag
     3241 tacacgtggc tgcgctcgct gatgggccgc tacgaggact tctggctggt gaccggtcag
     3301 gccctgcgct tcgcgttacg ccggctcggc gtcgccgcgg gccagtccca gatcgagtcg
     3361 ttgatggagg cctatctcgc cctgagcccg ttccccgagg tgccggccgc gctccgggcg
     3421 ctccgcggga cgccgctggc catcctctcg aacggctccc ctcggatgct ggagagcgcg
     3481 gtgcggtcga gcgggctcgc gggcgccttc gaccacgtcc tctcggtgga cgcggtcggg
     3541 gtctacaaac cgtcggcgcg ggtgtacgag atggcccccc gggcgctcgg gctgagcgcg
     3601 ggggagatcg tcttcgtgtc gtcgaatggc tgggacgtgg cgggcgccgc cgcctacgga
     3661 ttcacgactt gctggtgcaa ccgcgccaac gcccccgccg aggagctgga cctgaccccg
     3721 gactacgagg tggaccgtct ggacgccatt ccggagaagc tcggactcta gcggcagaac
     3781 gtcctggggc gccgggcggg gtcaggacgg tccgcgcgct gcgaaacggt tcctacttct
     3841 tggccaggcg cttccgcttc acgtatgccc agatcttctt cgtcatctcc gaggggccga
     3901 tgggcttgga gcccatgacc gactcgagcg tgtcgtcgca gcccttgaag ttgatcgcgt
     3961 agccgccaaa cgccagcttc ttcttcgcca tccgtcattc ctccctcgtt aggttattgg
     4021 gggtgctgcc cggcgccccc cggaacggcc aggggtatta tggacaaact ccgagggaga
     4081 gaaaagggga aaatacaatc cctagggggt ctccacgagg gagagactac ccggtggggg
     4141 tatacccccg ggggggtccc ccccccgggc cctcagcgtc cgacgaaggc gggcatcacg
     4201 tcccgtgcga accaccggag ctgctccttg aactccgcgg gcggaatgcc ctcggcccag
     4261 tggatcatgc actggtcgag gccgggatag cgcgcctcga tctcgcggat cgtctcgatc
     4321 acctccgcgg gccgcccgac cagccaggcc ttctgctcga cgccctcgcg cagggtcggc
     4381 acgcgcgcgg gcgcgcctgg cgtgccccac gagcggccct gctcgtcggc gtagcggacg
     4441 aagccgaacg gggcgaacca cttgtagcgc tcgtcgtggg ccggctccag acgacggatc
     4501 gcctcctcgc gcgagtcggc caggtagagg cccacgcccc agcagagatc ctccccgagc
     4561 tccttcggcc gcccgtgacg cacgcaggcg tcgcggtagg cgcgtgcgac ctggtcgagc
     4621 accttctctc cgttgagggt gaccatcgcc ttgagaccca tgcgggccat gtagtcgatc
     4681 gtcttgccgc tcgcgatcgg catccagatc tcgaccggcc ggtggatcgg ccgcgggacg
     4741 caggtgactt cacggaggcg atagccgcgg tagtccacgt cgggcgggat cacgtagtgc
     4801 ttgccccggt ggctccacga ctcctcgttg aagcacttga ggagcacctc catctgctcc
     4861 tcgaacagct cgcggttggc cgccgcgtcg aggagcggcg ccccgaagga ttcgacctcg
     4921 cgggtgtggt agccgcgacc gacgcccatg atgacccgcc cgtcggtgac gatgtcggcc
     4981 atg
//
LOCUS       DATMCV010000576         2169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_562678, whole genome shotgun sequence.
ACCESSION   DATMCV010000576 DATMCV010000000
VERSION     DATMCV010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562678"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VNU03_13310"
     CDS             <1..767
                     /locus_tag="VNU03_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWP75494.1"
                     /translation="ARSEIHGDRLVIVPHGILHYLPFAALRSPAGRWLVEDFALSTLP
                     SASVLRYLVDKGAGASARALVVGNPDLGPALALPWAEREARMVGQHEQESTVLVRADA
                     TEAQVKTLLPTVGLVHLATHGELRESDPLSSAILLVPGGGEDGRLEVREVFGLDLHAR
                     LVVLSACETGLGQLSRGDELVGLQRAFLYAGTPAVVTTLWKVDDRASFELIRAFYDRL
                     DAGGPVVALRQAQLETQRAFPHPFAWAAFGLTGVSR"
     gene            851..>2169
                     /locus_tag="VNU03_13315"
     CDS             851..>2169
                     /locus_tag="VNU03_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HWP75495.1"
                     /translation="MTDVELPRMLHVAFVRSPHAHARIRRIDTDAARAAAGVAAVALG
                     ADPDVASHRIEARSALPGYVMTGQPILAWPEVRFAGEAVAAVVARDRYAAEDAAALVS
                     VDWEPLPAALDLVDPARSGAVVHEAAPGNVLLARRFESGQVEATLASAAAVIERVFRT
                     NRQTAAPLEGRAGVADWSAGEGKLTLWLGTQVPHLVRHALAEMLALPENRIRVVAPDV
                     GGGFGVKAVVYPEEVALCVLAMRLGRPVKWVEQRREGFLASVHARDHHYAARAGFDRD
                     GRLLALDARIACNAGAYSVFPWTAGIEALMAGGLLAGPYKVPHYRCEVTAVATHTTPA
                     GPYRGVARPATTFVMERLLDLGARALGLDPVAVRRVNLIGPADLPYTSATRLVHDCGS
                     YPLCFEKAVEAIDYPAFRIEQARLRREGRYLGIGLASYNELTGLGQAA"
BASE COUNT          237 a          798 c          826 g          308 t
ORIGIN      
        1 cggcgcggtc ggagatccac ggcgaccggc tggtgatcgt gccgcacggg atcctgcact
       61 acctgccgtt cgccgcgctc cgctcgccgg ccggccgctg gctcgtggag gacttcgcgc
      121 tgagcacgct gccgagcgcg agcgtgctgc gctacctggt cgacaagggt gccggcgcct
      181 ccgcccgggc cctggtggtc ggtaacccgg acctgggccc ggctctcgcc ctgccgtggg
      241 ccgagcggga ggcccggatg gtcggccagc acgagcagga gtcgaccgtg ctggtccgcg
      301 ccgacgcgac cgaggcccag gtcaagacgc tgctcccgac ggtggggctc gtgcatctcg
      361 ccacccacgg cgagctcagg gagtcggacc cgctctcctc cgcgatcttg ctggtgccgg
      421 gcgggggcga ggacggccgg ctcgaggtgc gcgaggtctt cgggctcgat ctgcacgcgc
      481 gcctggtggt gctctcggcc tgcgagaccg ggctcggcca gctctcacgc ggcgacgagc
      541 tggtgggtct ccagcgggcg ttcctctacg cgggcacgcc ggcggtggtg accacgctgt
      601 ggaaggtcga cgaccgcgcg agcttcgagc tgatccgcgc tttctacgat cgcctggatg
      661 cgggcgggcc ggtggtcgcg ctccgccagg cccagctcga gacccagcgc gcgtttcccc
      721 atccctttgc ctgggccgcc ttcggcctga ccggcgtctc gcgctgagcg gcgccggccc
      781 ctcgagcatc tttggggcgc cgctgccccg caaggaggat gcgcggctcg tcgccggcca
      841 ggggcgctac gtgaccgacg tggagctgcc gcgcatgctc cacgtcgcct tcgtgcggag
      901 cccccacgcg cacgcgcgga tccgccggat cgacacggat gcggcgcgcg cggcggccgg
      961 cgtggccgcg gtcgccctcg gcgccgaccc ggacgtggcg tcgcatcgca tcgaggcgcg
     1021 ctcggccctg cccggctacg tgatgaccgg gcagccgatc ctggcctggc cggaggtccg
     1081 cttcgcgggt gaggcggtcg cggcggtggt cgcccgcgac cgctacgcgg cggaggacgc
     1141 ggccgcgctg gtgtcggtgg actgggagcc gctcccggcc gcgctcgacc tcgtcgatcc
     1201 ggcccggagc ggcgcggtcg tgcacgaggc cgcgcccggc aacgtgctcc tggcgcgccg
     1261 gttcgagagc ggccaggtgg aggcgacgct cgcgtcggcg gccgcggtga tcgagcgcgt
     1321 cttccggacg aaccggcaga ccgcggcccc gctcgagggg cgcgccggcg tggccgactg
     1381 gagcgcgggc gaaggcaagc tcaccctgtg gctcggcacc caggtcccgc acctggtgcg
     1441 gcacgcgctc gccgagatgc tcgcgttgcc cgagaaccgc atccgggtcg tggcccccga
     1501 cgtcgggggc ggcttcgggg tgaaggcggt ggtctatccg gaggaggtgg ctctgtgcgt
     1561 gctggccatg cgcctgggcc gcccggtgaa gtgggtggag cagcggcgcg agggcttcct
     1621 cgcctccgtg cacgcgcgcg accaccacta cgcggcgcgg gccggcttcg atcgcgacgg
     1681 ccgcctgctg gcgctcgacg cgcggatcgc gtgcaacgcc ggtgcgtact cggtcttccc
     1741 gtggaccgcg ggcatcgagg ccctgatggc cggcggtctc ctcgccggtc cctacaaggt
     1801 cccgcactac cgctgcgagg tgaccgcggt ggccacgcac acgacacccg cgggccccta
     1861 ccgcggggtg gcgcgcccgg ccaccacctt cgtgatggag cggctgctcg acctgggcgc
     1921 gcgcgccctc ggcctcgatc cggtcgcggt gcgccgggtg aatctgatcg ggcccgcgga
     1981 cctgccctac acgtcggcga cccgtctcgt ccacgattgc ggcagctatc cgctctgctt
     2041 cgagaaggcg gtggaggcga tcgactaccc ggccttccgc atcgagcagg cccgcctgag
     2101 gcgggagggc cgctatctcg gcatcggcct cgccagctac aacgagctga ccgggctcgg
     2161 ccaggccgc
//
LOCUS       DATMCV010000577         4242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_828057, whole genome shotgun sequence.
ACCESSION   DATMCV010000577 DATMCV010000000
VERSION     DATMCV010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828057"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VNU03_13320"
     CDS             complement(<1..421)
                     /locus_tag="VNU03_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HWP75496.1"
                     /translation="MTTSRGMVPADLTRIRFVSDAQVSPDGRVVAFVVTTLSEERDEY
                     LSQIWLVDGAGGEPRRFTTGPRRDTMPRWSPDGTRLAFVSEREGKKKGQLHVMPATGG
                     EPIRLTDLRHGVSAPAWSPDGTRLAFASRVGGWVEPES"
     gene            complement(483..680)
                     /locus_tag="VNU03_13325"
     CDS             complement(483..680)
                     /locus_tag="VNU03_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75497.1"
                     /translation="MLTFLIRCATWALIAWLVSMNPVVTAHARHAVAGWHAAWKIEAP
                     TCGVRAYGLLFRANDCETDDQ"
     gene            899..2089
                     /locus_tag="VNU03_13330"
     CDS             899..2089
                     /locus_tag="VNU03_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWP75498.1"
                     /translation="MRPVIGTLLAVTLALVLASPASAQAPPPWKQGQPADLANSTLAP
                     IAQPPAPAAASEIPVDKIKVPRGFKVSLWASGINNARAMVWGDNGTLFVSSRVAGNVY
                     AVVDRGGRREVKVIAKGLNLPNGVAFKDGTLYVAEVSRITRMPGIEAKLDNPPAPEVV
                     YDILPRDLPHGWKYLAFGPDGKLYFNIGAPCNICVPPDTHANISRVNPDGTGFEYVAH
                     GVRNSVGFDWHPVTKELYFATHGRDWLGDDAPSDRLDVASRKGLHFGFPYCHQGDIPD
                     PDFAQGRSCGEFAPPLLKTGPHVAGNGVQFYTGSMFPPEYRNRIFLAQRGSWNRSEKV
                     GFRVMMVTLRPRDVPKYEVFAEGWLQGDQIWGRPVYTQQMKDGSLLIADDYAGAIYRV
                     AYQP"
     gene            2107..2688
                     /locus_tag="VNU03_13335"
     CDS             2107..2688
                     /locus_tag="VNU03_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWP75499.1"
                     /translation="MGVAAVALLLGAAAAPGADLEAGRRKAQVCAACHGADGNATIPG
                     TPSLAGQPVFFTHWQLIKYRDGRRKDPQMSPLAERLDDADMADLAAFYAAQPARARPA
                     TTDAARVAAGQRLAEQHHCTSCHRPGLTGQQQAARLAGQDFAYLLRLLRAFKARTASD
                     LDGTMTVATQPLQDDEIVSLVHFLASLGTDTGR"
     gene            2735..3229
                     /locus_tag="VNU03_13340"
     CDS             2735..3229
                     /locus_tag="VNU03_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75500.1"
                     /translation="MLALLVALIALVVAAPAVAQVQVHVDIGFHLPAPPRLVVVPQVP
                     AVQYVPTPAAPSNLFFYNGQYWAFSAGGWYVSGGYNGPWVVVGPQFVPRPVLLVPVQY
                     YHVPPGHWKQWERARAPHWKEEWGPEWADKRAWKERDRDRGRDDDDDHPGHGKGKGKG
                     RGKD"
     gene            complement(3226..>4242)
                     /locus_tag="VNU03_13345"
     CDS             complement(3226..>4242)
                     /locus_tag="VNU03_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75501.1"
                     /translation="GEHVTLLGPSGCGKTTTLRAIAGLEPPESGEIRIDGQPVFSSSP
                     RIRVPPEQRGLSMVFQSYAIWPHMTVFDNVAYGLRVRKMPEAEIRERVAWALGLVQMR
                     ELAARSASKLSGGQQQRVALARAFVFGPAVLLFDEPLSNLDAKLRAEMRLEIKELQRA
                     LGITSVYVTHDLEEALAISDRIVVMRDGAIEQTGTPAEIYDLPRNSFVADFVGSANLI
                     EGRPRPELAGPGLVALETAGGAIVHGTAYGRRPGDRMCLSVRTVHVRLSRERPAGAVN
                     VWPATVRRRVFQGDFTQYHLDWDGRTLVVRGVTAEPVDEGSTVYLTVEPRHCVLLEDE
                     TGRR"
BASE COUNT          614 a         1529 c         1534 g          565 t
ORIGIN      
        1 cggactcggg ctccacccag ccgccgaccc gcgaggcgaa ggccagacgg gtcccgtcgg
       61 gcgaccacgc gggagcgctc acgccgtgcc gcagatcggt gaggcggatc ggctcgccgc
      121 cggtggcggg catcacgtgg agctggccct tcttcttgcc ctcgcgctcg gagacgaagg
      181 ccagccgcgt gccgtccgga gaccagcgcg gcatagtgtc ccgccggggg ccggtggtga
      241 agcgccgggg ctcgccgccg gctccgtcca cgagccagat ctgcgagagg tactcgtccc
      301 gctcctcgga gagcgtggtg acgacgaatg cgacgacgcg gccgtcgggc gagacctggg
      361 cgtcgctgac gaagcggatg cgggtgagat cggcgggcac catgccgcgg gaggtggtca
      421 tcgcgagcgc atcataccgc gggcgccgcg gcccgggcag ccgtctactc ccaggcggct
      481 gtctactggt cgtcggtctc gcagtcgttc gcgcggaaga gcaggccgta ggcgcggacg
      541 ccgcacgtcg gcgcctcgat cttccaggcc gcgtgccacc ccgccaccgc gtggcgcgcg
      601 tgggcggtga ccaccgggtt catgctgacc agccaggcga tcagcgccca ggtcgcgcag
      661 cggatgagaa aggtcagcat ggcgtgatca ccgcccccgg tctgcggcaa ggccgatgcc
      721 agcggcttga ggctcgtgct gccgggtact tgcggcgcgc tcggccgcgg ggcgctgacg
      781 ctccccgtca cccggtgccc accttgacaa ccgttcgtgg cgagacgtat ggtcccgccg
      841 gacgggctcg atgcgagccc gcgacccccc accaggaccc gcgaagggga ggctcaccat
      901 gcggcccgtc atcgggacac tgctcgccgt cacgctcgcg ctcgtcctcg cctccccggc
      961 ctcggcccag gcgccgcctc cgtggaagca gggccagccc gccgatctcg ccaactcgac
     1021 cctggccccg atcgcccagc cgcccgcccc ggcggccgcg agcgagatcc cggtggacaa
     1081 gatcaaggtg ccgcgcggct tcaaggtctc gctgtgggcg agcgggatca acaacgcgcg
     1141 cgccatggtg tggggcgaca acggcacgct cttcgtcagc agccgggtgg ccggcaacgt
     1201 gtacgcggtg gtggaccggg gcggccggcg cgaggtgaag gtcatcgcca aggggctcaa
     1261 cctgcccaac ggggtcgcct tcaaggacgg caccctctac gtcgcggagg tctcgcggat
     1321 cacccggatg ccggggatcg aggcgaagct cgacaacccg ccggccccgg aggtggtcta
     1381 cgacatcctg ccgcgcgatc tcccgcacgg ctggaagtac ctcgcgttcg gaccggacgg
     1441 caagctctac ttcaacatcg gcgcgccctg taacatctgc gtgccacccg acacccacgc
     1501 caacatctcc cgggtgaacc cggacggcac cggcttcgag tacgtggccc acggggtgcg
     1561 caacagcgtg ggcttcgact ggcacccggt gacgaaggag ctgtacttcg cgacccacgg
     1621 acgcgactgg ctgggcgacg acgccccgag cgaccggctc gacgtggcgt cgaggaaggg
     1681 gctgcacttc ggcttcccgt actgccacca gggcgacatc ccggatcccg acttcgccca
     1741 gggccgctcg tgcggggagt tcgcgccgcc gctgctcaag accggtcccc acgtggccgg
     1801 caacggggtg cagttctaca ccggctcgat gttcccgccc gagtaccgca accgcatctt
     1861 cctcgcccag cgcggctcgt ggaaccggag cgagaaggtc ggcttccggg tgatgatggt
     1921 caccctccgg ccccgcgacg tgccgaagta cgaggtcttc gccgagggat ggctgcaggg
     1981 cgaccagatc tggggccgcc cggtctacac ccagcagatg aaggatggct cgctgctgat
     2041 cgcggacgac tacgcgggcg cgatctaccg ggtcgcctac cagccctgac gcccgcccgg
     2101 ctcgcgatgg gggtcgcggc cgtcgccctg ctgctggggg cggccgcggc gcccggggcc
     2161 gatctggagg cggggcgccg caaggcccag gtgtgcgcgg cctgccacgg tgcggacggc
     2221 aacgcgacca ttcccggcac gccctccctc gccgggcagc ccgtgttctt cacgcactgg
     2281 cagctcatca agtatcgcga cgggcggcgg aaggacccgc agatgtctcc gctcgcggag
     2341 aggctggacg acgcggacat ggcggacctg gccgccttct acgccgcgca gccggcgcgg
     2401 gcgcgccccg ccaccaccga cgcggcgcgg gtcgccgcgg ggcagcggct cgcggagcag
     2461 catcactgca cgtcgtgcca ccggcccggg ctcaccggcc agcagcaggc cgcgcgcctg
     2521 gccgggcagg acttcgccta cctgctgcgg ctcctgcggg ccttcaaggc gaggaccgcc
     2581 tcggacctcg acgggacgat gacggtggcg acccagcccc tccaggacga tgagatcgtg
     2641 agcctggttc atttcctggc gagcctgggc acggatacgg ggcgctaggc tcaaaaaatc
     2701 caccgggcga caatacgggg acaggaggat ccccatgctc gccttgctcg tggcattgat
     2761 cgcgctcgtg gtcgcggctc ccgccgtggc gcaggtgcag gtccacgtgg acatcggctt
     2821 ccacctgccc gcgccgcccc ggctggtcgt ggtgccccag gtcccggcgg tgcagtacgt
     2881 gcccacgccg gccgcgccga gcaatctgtt cttctacaac ggccagtact gggcgttctc
     2941 cgccggcggc tggtacgtga gcggcggcta caacggcccc tgggtggtcg tcgggcccca
     3001 gttcgtgccg cggccggtgc tgctcgtgcc ggtgcagtac tatcacgtgc cgcccggcca
     3061 ctggaagcag tgggagcgcg cccgcgctcc gcactggaag gaagagtggg gcccggagtg
     3121 ggccgacaag cgggcctgga aggagcgcga tcgcgaccgg ggccgggacg acgacgacga
     3181 ccacccgggc cacgggaagg gcaagggcaa ggggcgcggc aaggactagc gtcgcccggt
     3241 ctcgtcctcc agcagcacgc agtggcgcgg ctcgacggtc aggtagaccg tcgagccctc
     3301 gtccaccggc tccgcggtca cgccccgcac gacgagggtc cgcccgtccc agtcgaggtg
     3361 atactgggtg aagtcgccct ggaacacacg gcggcgcacc gtcgccggcc acacgttgac
     3421 cgctccggcc gggcgctcgc gcgagaggcg cacgtggacg gtgcgcaccg atagacacat
     3481 ccggtcgccg ggccgtctcc cgtaggcggt gccgtgcacg atcgcgccgc ccgcggtctc
     3541 gagggccacg aggcccgggc cggccagctc cggccgcggc cggccctcga tcaggttggc
     3601 cgatcccacg aagtcggcga cgaacgagtt gcggggcaag tcgtagatct cggccggggt
     3661 gccggtctgc tcgatcgccc cgtcgcgcat gaccacgatg cggtcggaga tcgcgagggc
     3721 ctcctccagg tcgtgggtga cgtacaccga ggtgatgccg agcgcgcgct gcagctcctt
     3781 gatctcgagc cgcatctcgg cgcgcagctt cgcgtccagg ttgctgaggg gctcgtcgaa
     3841 gagcagcacc gccggcccga acacgaaggc gcgggcgagg gccacccgct gctgctggcc
     3901 gcccgagagc ttcgaggcgc tgcgggcggc cagctcgcgc atctgcacga ggccgagcgc
     3961 ccaggcgacc cgctcccgga tctccgcctc cggcatcttc cgcacccgca ggccgtaggc
     4021 cacgttgtcg aacacggtca tgtggggcca gatcgcgtag gactggaaca ccatcgacag
     4081 gccgcgctgc tcgggcggga cgcggatccg cggggaggac gagaagaccg gctggccgtc
     4141 gatccggatc tcgcccgact cgggcggctc gagcccggcg atggcccgga gcgtggtggt
     4201 cttgccgcag cccgaggggc cgagcagggt cacgtgctcg cc
//
LOCUS       DATMCV010000578         4098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_828077, whole genome shotgun sequence.
ACCESSION   DATMCV010000578 DATMCV010000000
VERSION     DATMCV010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828077"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VNU03_13350"
     CDS             <1..268
                     /locus_tag="VNU03_13350"
                     /inference="COORDINATES: protein motif:HMM:NF036805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP75502.1"
                     /translation="HGPVIEDGPGKIQEFIEHRLLRERQILETLGGGPLTIPEIVKVI
                     YADVDTRLHRVAAMSVHSHLRKLKSESRVSEEPVTGAPSRWTLV"
     gene            286..1926
                     /locus_tag="VNU03_13355"
     CDS             286..1926
                     /locus_tag="VNU03_13355"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75503.1"
                     /translation="MLLTNGRIHTLDAAGSVVDSLVVRDGRVAFAGRRADVNPAARED
                     VIDLGGRTVLPGLVDGHGHLMLLARARLELDLAAAGSEDEIARMVAAAAARLRPGEWI
                     AGRGWDQTRWPGQRFPARVSLDRAASGHPVALVRIDGHATWTSGAALERAAITRRSGD
                     PAGGRIVKDEHGEPTGLLIDLAQDLIRSLVPPPSPERFDAAVEAVVAECLAKGLTGTH
                     EPGLDLSAVASYTRLIGRDRFPFRVFAALSGKKAWGEYRERGRPESIGDGHLTVGAIK
                     LWLDGALGSRGAALHAPYCDDPANTGLVLVPPEEVLRLTREAAALGFHVWVHAIGDRA
                     NTLVLDVFERALADPGPRPRLRVEHAQILAPADIPRFARLGVVPSMQPTHCTSDMRWA
                     GERLGPERLVGAYAWRSVLDSGAYVAGGSDFPVEDANPFHGIHAAVTRAPREGPDPGW
                     QPAQRMTRDEAVRSFTIWNARSIGREAELGSLEPGKRADLIALSEDVFTCAPERIAGI
                     APVLTMVGGAVAHADQEVGASLSTSFTRSGRSVSDTSA"
     gene            complement(1854..2639)
                     /locus_tag="VNU03_13360"
     CDS             complement(1854..2639)
                     /locus_tag="VNU03_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWP75504.1"
                     /translation="MTCDEARALLLDAQRGRLPADRRALLDAHVAGCEACAHEEAAET
                     LLSELLEHRLPQHAAPLALKRRLAATWPDAAAPAPPWWAGWRRALVPALALGVLLLAA
                     LPLYYPPSGGAGAVMVTEAVNDHLRLLASQHPLDIESGGMHQVKPWFEGRLDFAPVVR
                     FLGDDDFPLRGGAVGYYVDRKAAVFVFYRRLHPMTLLVFRGDGLPWARRGLEPIGRVQ
                     AQVATSRGFNAILWREGELGYALVSDTDLPDLVKLVERLAPTS"
     gene            complement(2636..3202)
                     /locus_tag="VNU03_13365"
     CDS             complement(2636..3202)
                     /locus_tag="VNU03_13365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP75505.1"
                     /translation="MNGAYDLGMAGEQPADAAALGREALAYADTLYNLARYLTRSPTD
                     AQDLVQETYARALSASHQFTPGSNLKAWLFRILRNTFLSQYRHERHNPTVGGLDTVAP
                     ATPAPETVQWLRGDVELDRLRKVVGEEIERALTSLSEEARTVILLDLEGLTEVEVAQV
                     VGCAVGTVKSRLARARAALREKLRDYAP"
     gene            3316..3648
                     /locus_tag="VNU03_13370"
     CDS             3316..3648
                     /locus_tag="VNU03_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HWP75506.1"
                     /translation="MKILLTMMALLLAGPVAAPAQMESTQVEIRAHGFSAPALTVKAG
                     TTVTWINHDDDAHTVTSTANAFRSPGLDTDETFSFTFTQPGTYEYFCTLHPLMTGKVV
                     VTPERSGT"
     gene            3645..>4098
                     /locus_tag="VNU03_13375"
     CDS             3645..>4098
                     /locus_tag="VNU03_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWP75507.1"
                     /translation="MSDASEGHELDRRGFLQCMAWTGTGLVWSVVGGVPGSRVLGPRA
                     AQAQGAGQSFSFVQISDTHIGFKGAANPDALGTFEAAIARIKALDPKPAFLIHTGDIT
                     HAQKAGAFDTVSEALKGTGVERTFYVPGENDVFADGGTEYLNRFAVGGP"
BASE COUNT          566 a         1507 c         1489 g          536 t
ORIGIN      
        1 gcacgggccg gtgatcgagg acgggccggg caagatccag gagttcatcg agcaccggct
       61 cctccgcgag cgccagatcc tcgagacgct cggcgggggc cccctgacca tcccggagat
      121 cgtcaaggtc atctacgccg acgtcgacac ccggctccac cgcgtggccg ccatgtcggt
      181 gcactcgcac ctgcgcaagc tcaagagcga gagccgggtg agcgaggagc cggtgaccgg
      241 cgcgccgtcc cgctggacgc tggtctagag cgccacgctc gcccagtgct cctgaccaac
      301 gggcggatcc acacgctgga tgccgccggc tcggtggtgg actctctcgt cgtccgcgac
      361 ggccgagtcg ccttcgcggg ccggcgcgcc gacgtcaatc cggccgcgcg cgaggacgtc
      421 atcgacctgg gcggccgcac cgtgctgccc ggcctggtcg acgggcacgg ccatctcatg
      481 ctgctcgccc gcgcgcggct cgagctggac ctggccgcgg ccggctcgga ggacgagatc
      541 gcccggatgg tggccgcggc ggcggcgcgg ctccgtccgg gggagtggat cgccgggcga
      601 ggctgggacc aaacgcgctg gcccggccag cgcttcccgg cgcgtgtctc gctggaccgg
      661 gccgcgtccg gccatccggt cgcgctggtg cggatcgacg gccacgccac ttggaccagc
      721 ggggccgcgc tggagcgcgc cgcgatcacg cgccgctccg gcgacccggc gggcggccgc
      781 atcgtgaagg acgagcacgg cgagcccacc gggctcctga tcgacctggc ccaggacctg
      841 atccgctccc tcgtgccgcc gccgtccccc gagcgcttcg acgccgcggt ggaggcggtc
      901 gtcgcggagt gcctggccaa gggcctcacc gggacccacg agcccgggct cgacctgtcc
      961 gcggtcgcct cgtacacgcg cctgatcggc cgcgaccggt ttccgttccg ggtgttcgcg
     1021 gccctcagcg gcaagaaggc gtggggcgag tatcgcgagc gcgggcggcc cgagtcgatc
     1081 ggcgacggtc acctgacggt gggcgcgatc aagctctggc tcgacggcgc gctcggctcg
     1141 cgcggcgccg cactgcacgc gccctactgc gacgaccccg cgaacaccgg cctggtcctg
     1201 gtgccgcccg aggaggtgct gcgcttgacg cgcgaggccg ccgcgctcgg cttccacgtc
     1261 tgggtgcacg ccatcggcga ccgcgccaac acgctggtcc tcgacgtgtt cgagcgggcg
     1321 ctggccgacc cgggcccccg cccccgcctg cgggtcgagc acgcgcagat cctggctccc
     1381 gccgacatcc cgcgcttcgc ccgcctcggc gtggtgccga gcatgcagcc cacgcactgc
     1441 acgtccgaca tgcggtgggc gggggagcgt ctggggccgg agcgcctcgt gggcgcctac
     1501 gcctggcgct cggtgctgga ctcgggggcc tacgtggcgg gcggctcgga cttcccggtg
     1561 gaggacgcca atcccttcca cggcatccac gccgcggtga cgcgcgcgcc gcgcgagggt
     1621 ccggaccccg gctggcagcc cgcgcagcgc atgacccgcg acgaggcggt ccgctcgttc
     1681 accatctgga acgcacgctc catcggccgc gaggccgagc tgggatcgct cgagccgggc
     1741 aagcgggccg acctgatcgc gctctccgag gacgtcttca cgtgcgcgcc cgagcggatc
     1801 gccggcatcg cgccggtgct caccatggtg ggcggcgcgg tcgcccacgc cgatcaggag
     1861 gtcggggcga gcctctcgac gagctttacc agatctggca gatcggtgtc ggacaccagc
     1921 gcgtagccca gctcgccttc ccgccacagg atcgcgttga acccccggga ggtcgcgacc
     1981 tgcgcctgca cgcgtccgat cggctcgagc ccgcgacggg cccacgggag cccgtcgccc
     2041 cggaagacca gcagcgtcat cgggtgcagc cggcggtaga agacgaagac ggccgccttg
     2101 cgatccacgt aatagccgac cgcgccgccc cgcagcggga agtcgtcgtc gcccaggaag
     2161 cgcaccaccg gcgcgaagtc gaggcgcccc tcgaaccacg gcttgacctg gtgcatgccc
     2221 ccgctctcga tgtcgagcgg atgctggctc gcgagcaggc gcaggtggtc gttgaccgcc
     2281 tcggtcacca tgaccgcgcc ggctccgccc gacggcgggt agtagagcgg cagcgcggcc
     2341 agcagcagca cgcccagcgc cagggccggg accagggcgc ggcgccagcc cgcccaccac
     2401 gggggcgccg gcgcggccgc gtcgggccag gtcgcggcga gccgacgctt cagcgcgagc
     2461 ggcgccgcgt gctggggcag acggtgctcg agcagctcgc tcaggagtgt ctcggccgcc
     2521 tcctcgtgag cgcaggcctc gcacccggcc acgtgagcgt cgagcagcgc acgccgatcc
     2581 gcgggcaggc gcccccgctg ggcgtccagc agcagcgcgc gcgcctcgtc gcaggtcatg
     2641 gcgcgtaatc ccggagcttc tcgcgcaggg ccgcgcgggc ccgcgcgagt cgcgacttca
     2701 cggtgcccac cgcgcagccg accacctgcg cgacctcgac ctcggtgaga ccctcgaggt
     2761 ccagcaggat cacggtgcgc gcctcctcgg agagactcgt gagggctcgc tcgatctcct
     2821 caccgaccac cttgcgcagc cggtccagct cgacgtcgcc gcgcagccac tggaccgtct
     2881 ccggggcggg cgtggcgggc gcgaccgtgt cgaggcctcc cacggtcgga ttgtgacgct
     2941 cgtgccgata ctggctgagg aaggtgttgc gcaggatgcg gaagagccac gccttgagat
     3001 tgctccccgg ggtgaactgg tgcgaggccg agagcgcccg cgcgtaggtc tcctgcacga
     3061 ggtcctgcgc gtcggtgggg ctccgggtga ggtagcgggc gaggttgtag agggtatccg
     3121 cgtacgccag ggcctcgcgc ccgagcgccg cagcgtcagc cggttgctcg cccgccatgc
     3181 cgagatcata ggctccgttc acgtgcttcc gccatctatc ctccgcgctc gtcctccgta
     3241 acgcagggcg gcccgattcg ttccacggaa cgatcggccc ggccccgcgt tggcagcggc
     3301 gaggaggaac cgatcatgaa gatactgctc acgatgatgg cgctgctgct ggcgggtccg
     3361 gtcgcggcgc cggcccagat ggagagcaca caggtggaga tccgggccca cgggttcagc
     3421 gccccggcgc tgaccgtgaa ggccgggacc accgtcacct ggatcaacca cgacgacgac
     3481 gcccacaccg tgacgtcgac cgccaacgcc ttccggtcgc cgggcctgga caccgacgag
     3541 acgttctcct tcaccttcac gcagccgggc acctacgagt acttctgcac gctgcacccc
     3601 ctgatgaccg gcaaggtggt cgtgacgccg gagaggagcg gcacatgagc gacgcgagcg
     3661 aagggcacga gctggatcgg cgcggcttcc tgcagtgcat ggcgtggacc ggcaccggcc
     3721 tggtgtggtc ggtggtcggg ggtgtgccgg gctcgcgcgt gctcgggccc cgggcggccc
     3781 aggcgcaagg ggcggggcag agcttcagct tcgtccagat cagcgacacc cacatcgggt
     3841 tcaaaggcgc ggcgaacccg gacgcgctgg gcaccttcga ggccgccatc gcgcgcatca
     3901 aggcgctcga ccccaagccg gccttcctga tccacaccgg cgacatcacc cacgcccaga
     3961 aggcgggtgc gttcgacacg gtgagcgagg cgctcaaggg caccggcgtc gagcggacct
     4021 tctacgttcc gggagagaac gacgtgttcg cggacggcgg caccgagtac ctgaaccggt
     4081 tcgcggtggg cgggccgg
//
LOCUS       DATMCV010000579         3231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_498016, whole genome shotgun sequence.
ACCESSION   DATMCV010000579 DATMCV010000000
VERSION     DATMCV010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498016"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..2504
                     /locus_tag="VNU03_13380"
     CDS             195..2504
                     /locus_tag="VNU03_13380"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWP75508.1"
                     /translation="MDTPLGSPLRRKEDQRLVTGRGRYVEDLRFPGMLHAAFVRSPHA
                     HARVRSIDTDGARALGGVIAVYTARDLPECARAIPPSIAAPAGFRPTAQPVFADPLVR
                     YVGEVVAAVVAGDPYVAADAAAAVLVDYEPLPAAGTMERALEPGAPRVFDGWPDNVAG
                     LSTARVGDAAAGFAGSDVVVEARLGIARVHGVPIETRGIVATPEGPDGRLTLWTSSQS
                     PYGLRAVVAGAFGIAEEHVRVVVVDTGGGFGIKGHTYSEDLIVAAAARRLGRPVKWAE
                     SRREHFLTASPDRGQRHVARLGLTREGRITAVETRFTREHGAYIASGEVVTRNTINHL
                     PGPYRIPSLEIAASNVVTNAVFGAAYRGSGRPEANFVLERLIDRGARALGMDPAELRR
                     RNMIRAEEMPYRTGLVYRDGTPVSYDPADYVASFDRLLADFGYPEWRRRQVETASGAR
                     PIGVGLAAYVQGTGVGPFESADVRIDSGGTVHVYIGVSSQGQSHETTMAQIAAAELGV
                     DPERVFVIAADTTLLPYGNGTGGSRVAANAGPSVARTAREVAGKARVVAAEMLECAPA
                     DVVLAAGRAHVAGLPERGVALGDLSRAAVRSKTLLREARPGLQACGYFAPETVTFAFG
                     AQACAVEVDVETGRVRILRYLAAHDCGRAINPMIVDGQLHGGLAGGIGTALMEDIVYD
                     ADGQLLTGSLMDYAIPSAADLPSFETRSLAFPSTRNDLGIKGVGESAIIAPPAAIANA
                     VEDALHSRGADILEVPITPSRVWRALQAS"
     gene            2509..>3231
                     /locus_tag="VNU03_13385"
     CDS             2509..>3231
                     /locus_tag="VNU03_13385"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWP75509.1"
                     /translation="MSQPPPFFLADGDRFIPSASARGPWSARHQHGGPPAALLARAFA
                     RQAGPDVQIARLTFDFLRPVPLAALTVATRVVRAGSKVKRLAGTVLAEDGAAVIEATC
                     LAMRLAPNSVVVPLETPTPPARPADSAPFELPLMSGDEGYHRAVEWRIAQGIWGRGPV
                     SAWLRLRAPLVAGETTSPLESLVAIADSASGLAVIVDPARATFVSGDLTIALHRVPAG
                     EWMCLDAATTGESHGIGLTRARL"
BASE COUNT          391 a         1183 c         1219 g          438 t
ORIGIN      
        1 gtcggggtga taggcgcggc ccggcgcggg cgtcgcacag aggaacggct ggtcggggac
       61 cgccgacgcg gtccccatga aggcctcgaa gacggtctgc acggctctcg actctcgccc
      121 acgcgccggg cgctgtcaat cgttggcgcg atcggctggc ggccgcgggc ctgatgtata
      181 ctcccgacgc gacgatggac actccgctcg gttccccgct gcgccgcaag gaagatcagc
      241 gtctcgtcac cggccgcggc cgctacgtcg aggatctgcg ctttcccggc atgctgcatg
      301 cggccttcgt gcggagcccg cacgcccacg cgcgcgtgcg ctcgatcgac actgacggcg
      361 cgcgggccct gggcggggtg atcgcggtgt acaccgcgcg cgatctcccc gagtgcgcgc
      421 gcgccatccc gccgtcgatc gcggcccctg ccggcttcag accgaccgcg cagccggtgt
      481 tcgccgatcc gctcgtgcgc tacgtgggcg aggtcgtggc cgcggtggtg gccggggatc
      541 cctacgtggc cgcggacgcg gccgccgcgg tgctcgtcga ctacgagccc ctgcccgccg
      601 cgggcaccat ggagcgcgcg ctcgagcccg gcgccccgcg cgtattcgac ggctggcccg
      661 acaacgtggc cggtctctcc accgcccgcg tgggcgacgc ggcggccggc ttcgccggct
      721 ccgacgtggt ggtggaggcg cggctcggca tcgcccgagt gcacggggtc ccgatcgaga
      781 cgcgcgggat agtcgccacg ccggaggggc ccgacggccg gctcaccctg tggacgtcga
      841 gccagagccc ctacggcctc cgcgcggttg tcgcgggggc cttcggcatc gccgaggagc
      901 acgtgcgcgt ggtggtggtc gacaccggcg gcgggttcgg catcaagggc cacacctact
      961 ccgaggacct gatcgtggcg gcggcagccc ggcggctggg gcggccggtg aagtgggccg
     1021 agtcgcggcg cgagcacttc ctcaccgcgt ctcccgaccg cggccagcgg cacgtggcgc
     1081 gcctcgggct cacccgggag ggccggatca ccgcggtcga gacgcgcttc acccgggagc
     1141 acggggccta tatcgcgagc ggcgaggtgg tgacccggaa caccatcaac cacctgccgg
     1201 gcccgtaccg cattccgagc ctcgagatcg ccgcgtccaa cgtggtgacc aacgcggtgt
     1261 tcggcgccgc gtatcgcggc tccggacggc cggaggccaa cttcgtgctg gagcggctga
     1321 tcgatcgggg ggcgcgggcg ctcggcatgg atcccgccga gctgcggcgc cgcaacatga
     1381 tccgggccga ggagatgccg tatcgcaccg gcctggtcta ccgcgacggc acgccggtct
     1441 cctacgatcc ggcggattac gtggccagct tcgatcgcct cctggccgac ttcggctacc
     1501 cggagtggcg ccggcgacag gtcgagaccg cctcaggcgc gcggccgatc ggagtggggc
     1561 tcgcggccta cgtgcagggc accggggtgg gcccgttcga gagcgcggac gtgcgcatcg
     1621 actcgggcgg gacggtgcac gtctacatcg gggtctcctc gcagggccag tcgcacgaga
     1681 cgacgatggc ccagatcgcg gcggccgagc tgggcgtcga ccccgagcgg gtgttcgtga
     1741 tcgcggccga taccacgctg ctcccgtacg gcaacggcac cggcggtagc cgggtggccg
     1801 ccaacgcggg gccgtctgtc gcccgcaccg cgcgcgaggt ggcgggcaag gcgcgggtgg
     1861 tggccgccga gatgctcgag tgcgcccccg ccgacgtggt gctggccgcg ggccgggcgc
     1921 acgtggcggg gctgccggag cgcggggtgg cgctgggcga tctctcgcgc gccgcggtgc
     1981 ggagcaagac gctcctgcgc gaggcgcggc cggggctgca ggcctgcggg tacttcgcgc
     2041 ccgagaccgt gaccttcgcg ttcggggcgc aggcctgcgc ggtggaggtg gatgtggaga
     2101 ccggtcgggt gcggatcctc cgctacctgg ccgcgcacga ctgcgggcgg gccatcaacc
     2161 cgatgatcgt ggacgggcag ctccacggcg gcctggccgg cggcatcggc accgcgctga
     2221 tggaggacat cgtctacgac gccgacggcc agctcctgac cgggagcctg atggactacg
     2281 cgatcccgag cgcggccgac ctgccgtcgt tcgagacgcg ctcgctcgcc ttcccctcca
     2341 cccggaacga cctcggcatc aagggagtgg gcgagagcgc gatcatcgcg ccgcccgccg
     2401 ccatcgcgaa cgcggtggag gacgcgctcc actcgcgggg cgccgacatc ctcgaggtgc
     2461 cgatcacccc ctcgcgcgtg tggcgggctc tccaagcgtc ctgagcgcgt gagccagccg
     2521 ccgccgttct tcctcgccga cggcgaccgg ttcattccct ccgcctcggc gcgcgggccc
     2581 tggagcgcgc ggcatcagca cggcggccca ccggccgcgc tcctcgcccg cgcgttcgcc
     2641 cgtcaggccg ggcccgacgt gcagatcgcg cgcctgacct tcgatttcct ccggccggtg
     2701 cccctggctg ccctgaccgt cgcgacgcgc gtcgtccgcg cggggtccaa ggtcaagcgc
     2761 ctcgcgggca ccgtcctggc cgaggacgga gccgccgtga tcgaggcgac gtgtctcgcc
     2821 atgcggctgg cgccgaatag cgtcgtcgtg cccctcgaga ccccgacccc tccggcgcgg
     2881 cccgcggaca gcgcaccctt cgagctgcct ctcatgtcgg gcgacgaggg gtaccaccgc
     2941 gcagtggagt ggcggatcgc gcaggggatc tgggggcgag ggccggtctc ggcgtggctc
     3001 cgcctgcgag ctcccctggt ggcgggcgag acgacctcgc cgctcgaatc cctcgtcgcc
     3061 atcgccgact ccgcgagcgg cctggccgtc atcgtcgatc cggcccgggc cacgttcgtg
     3121 agcggggacc tgacgatcgc gctccaccgg gtcccggccg gcgagtggat gtgtctggat
     3181 gcggccacga ccggcgagag ccacgggatc ggcctgacgc gggcacggct c
//
LOCUS       DATMCV010000580         5775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_297941, whole genome shotgun sequence.
ACCESSION   DATMCV010000580 DATMCV010000000
VERSION     DATMCV010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297941"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VNU03_13390"
     CDS             complement(<1..675)
                     /locus_tag="VNU03_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75510.1"
                     /translation="MHVWQRWAAAIAIGAVGLAGWAGAGRAQAPKAQWVVAIGEEGES
                     LDPPTSMLFTSEIYQQHLFDCLLGIEGEEFKPVPLLAEKWETINPTTWRFHLRKGVKF
                     HDGKPLTAEDVKYSFEQYADPKNRRSVFARGISKIEIRNASTVDLITSEPVSALLFNL
                     TRLQILPKDTREKAGVQAFSEKPIGTGPYKFVEWKRDQQLVLEANPSYWRGQVNPKRL
                     IFRAIRD"
     gene            873..2186
                     /locus_tag="VNU03_13395"
     CDS             873..2186
                     /locus_tag="VNU03_13395"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HWP75511.1"
                     /translation="MADATTPRSSEETKLLERARQRLPGGVLGTSRYADDVGFVVKHG
                     KGSKIYDVSGREYIDYVMASGPMVLGHAHPAVVAAVRKQIEDGTTYFMVTEPIIELAE
                     EICRAVPCAEQVRFTSTGSEATFFALRVARTARQRDKILKFEGGYHGAHDYSMMSSAP
                     RSPKAFPAPVPDSSGIPQVLEDQVLIAPYNDLATVEGIVATHADELAAVIIEPFQRLI
                     PPQPGFLQGLREITRRHGILLVFDEVVTGFRLAYGGAQQYYGVVPDIACVGKIVGGGF
                     PLAAVCASAELMQPFDPGQDGKGDFISQSGTLNGNPIAAVAGLATLAELRKPGAYETL
                     HGTGRRLMAGLAELAQKSGLPAQVVGEPVVFDILFTSEPVTDYRSLQKADGGLARTFT
                     TELIKRGVVKNTQKMYMSLAHTDADVAKTLQACEDALKVLPKKRR"
     gene            complement(2261..2653)
                     /locus_tag="VNU03_13400"
     CDS             complement(2261..2653)
                     /locus_tag="VNU03_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75512.1"
                     /translation="METMPRKRSLAERWRDVRPTKTAMFWSWVVVVVLTLVVGFEWGG
                     WVTGKTARSMAEAAGHDAVVARLAPMCVTQATHDPQWAEKRKALKTTESWDRTDYVVK
                     QGWATLPAEQQADPRVAEQCVTLLLQHS"
     gene            complement(2770..3729)
                     /locus_tag="VNU03_13405"
     CDS             complement(2770..3729)
                     /locus_tag="VNU03_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75513.1"
                     /translation="MRPSWTRAFGMRRPALAPEAAGGAAALSLPARVGPRARPWWLDM
                     WVRMLTGKPLGAVGGAIVLVMLAAAVLADWIAPYGFAQTSLRERFIVASAAHWLGTDE
                     LGRDLLTRLIYGARISLYVGFGAIAIGSVLATAIGVVSGYLGGRFDLLVQRGVDAWMA
                     FPPLLLLLSIMSLLGPSVWNITVVLGIAFGIQNSRVVRGVALLVKEHSFVESARALGA
                     GHLRTTLTHILPNVVPTIIVVATTGLSTVILTEASLSFLGLGVPPPYPTWGGMLSLAG
                     LDHMYRAPWLAIWPAVALSLAVFGFNMLGDALRDLLDPRLKGG"
     gene            complement(3734..4678)
                     /locus_tag="VNU03_13410"
     CDS             complement(3734..4678)
                     /locus_tag="VNU03_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75514.1"
                     /translation="MKRYVLRRVLLAVPTLFLVSVMVFALMRFMPGDVATRMVEGHAY
                     APTLAALRHDLGLDRPVHTQYLDWIGGIVLRGDFGHSYWTRQPILDEFGRRFPVTLEL
                     ALLTILVSVVLGVVIGIVSAARQDSAADYLGRVLAILALSVPYFGLAVLVVVLPSIYF
                     KWTPVWTYVAFTVDPLENLKIMLVPALVFGVTRAGPVMRIMRSALLDVLRQDYIRTAW
                     SKGLAERPIILRHALKNAMIPVISLIGLQMPLYIGGSVIIESIFRLPGVGLFFFEALT
                     RLDYPVVQSVNLIIAAMVVGLNLLIDLSYAFLDPRIRY"
     gene            complement(4703..>5775)
                     /locus_tag="VNU03_13415"
     CDS             complement(4703..>5775)
                     /locus_tag="VNU03_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75515.1"
                     /translation="PREAEEKFKDFNNPDAVIGTGPFVLKSYEKGVRMVFERNPTYFM
                     KGFPYLDGVTVDITPDAAARLAVLRAGKAELPHVWGWISPEEARSLKQTSPEMSITPY
                     KVIGQGFIYMRTDQPPFNDVRVRRAVSLAIDRKSWNDALLFGEGCVDAGPVPCALTDW
                     KLDAAKMEPAKAKYVVGYDPAEAKKLLAEAGYAKGFTMPIYHWPGYVVPWRNYYELAA
                     EDLGKIGITVELKPEEYGKYISTTALGKYDKAAMGPSTPFTEVDDFLFGRFYPELPTN
                     QSHVADGELTKMLVAQRREMDPRKRKQIVDDIQRYLADKAYYVYVPQWPQYVAHPPYV
                     KGFRHHDGYGLGMRLLYTWLDK"
BASE COUNT          930 a         1972 c         2005 g          868 t
ORIGIN      
        1 gtcgcggatg gcgcgaaaga tcaggcgctt ggggttgacc tggccgcgcc agtagctggg
       61 attggcctcc agcaccagct gctggtcgcg cttccactcc acgaacttgt aggggccggt
      121 gccgatgggc ttctcggaga acgcttgcac gccggccttc tcgcgcgtgt ccttgggcag
      181 gatctgaagg cgcgtcaggt tgaagagcag cgccgagacc ggctcggacg tgatcaggtc
      241 gacggtgctc gcgttgcgga tctcgatctt cgagatgccg cgggcgaaga ccgaacgccg
      301 attcttcggg tccgcgtact gctcgaagga gtatttcacg tcctccgcgg tgagcggctt
      361 gccgtcgtgg aacttcaccc ctttacggag gtggaagcgc caggtcgtgg ggttgatggt
      421 ctcccacttc tcggccagca gcggaaccgg cttgaactcc tcgccctcga tgcccagcag
      481 gcagtcgaag aggtgctgct ggtagatctc ggaggtgaac agcatgctgg tgggcgggtc
      541 caggctctcg ccctcctcgc cgatggccac tacccactgg gccttggggg cctgggcccg
      601 gccggctccc gcccagcccg ccagccccac cgctccgatc gcgatcgccg ccgcccaccg
      661 ctgccagacg tgcatgccgg gtctccttgc cagggctatc gtttgccaaa gctctcgttc
      721 gaaggtcagc ttgagtgcta cttgtatacc agtaaagtgc aggcttgcca accggggggc
      781 cgggcctcgc cttgacagac gccggagccg agcctaggct gctttcgttc ccgacgcggt
      841 ccacgtgact ctcaccgaga cgaggtccag ccatggccga cgcgaccact ccccgatcca
      901 gtgaagagac caagctgctc gagcgtgcgc ggcagcggct gccgggcggc gtgctcggca
      961 cctcccgcta cgcggacgac gtcggcttcg tggtcaagca cgggaagggc tcgaagatct
     1021 acgacgtgag cggccgcgag tacatcgact acgtgatggc ctccggcccg atggtgctgg
     1081 gccacgccca ccccgcagtg gtggccgcgg tgcgcaagca gatcgaggac ggcaccacct
     1141 acttcatggt gaccgagccg atcatcgagc tggccgagga gatctgtcgg gcggtgccgt
     1201 gcgccgagca ggtgcgcttc acctccaccg gatccgaggc caccttcttc gcgctccgcg
     1261 tcgcccgcac cgcgcggcag cgcgacaaga tcctcaagtt cgagggcggc taccacggcg
     1321 cgcacgacta ctcgatgatg tcgtccgccc cgcgctcgcc gaaggcgttt cccgctccgg
     1381 tgccggactc gtccggcatt cctcaggttc tggaagacca ggtcctgatc gcgccctaca
     1441 acgacctcgc caccgtggag ggcatcgtgg cgacccacgc cgacgagctg gccgcggtga
     1501 tcatcgagcc cttccagcgg ctcatcccgc cccagcccgg gttcctccag gggctacgcg
     1561 agatcacccg gcggcacggc atcctgctcg tcttcgacga ggtggtgacc ggcttccgcc
     1621 tcgcctacgg tggcgcccag cagtactacg gggtggtgcc ggacatcgcc tgcgtcggca
     1681 agatcgtggg cgggggcttc ccgctcgccg cggtgtgcgc gtccgccgag ctgatgcagc
     1741 ccttcgatcc ggggcaggac ggcaagggcg atttcatctc gcagtctggt accctcaacg
     1801 gcaacccgat cgcggcggtc gcggggctcg ccacgctcgc cgagctgcgc aagcccggcg
     1861 cctacgagac cttgcacggg acggggcggc ggctgatggc cggcctcgcc gagctggcgc
     1921 agaagtcggg actgcccgcc caggtggtgg gtgagccggt ggtcttcgac atcctcttca
     1981 cgagcgagcc cgtcaccgac taccggtcac tgcagaaggc cgacggcggg ctggcccgca
     2041 ccttcaccac cgagctgatc aagcggggcg tggtgaagaa cacccagaag atgtacatgt
     2101 cgctggccca caccgacgcc gacgtggcca agaccctcca ggcctgcgaa gacgccctca
     2161 aggtcctccc gaagaagagg cgctagcgcg gccggggtca ggtcttacat tccgacattg
     2221 tcgcaatgta agacctgacc ccccgacgac ccccccgagc tcaggagtgc tggagcagga
     2281 gggtgacgca ctgctcggcc acgcgcggat ccgcctgttg ctccgcgggc aatgtggccc
     2341 agccctgctt gaccacgtag tcggttcgat cccacgattc ggtggtcttc aaggccttgc
     2401 gcttctcggc ccactgcggg tcgtgggtgg cctgggtcac gcacatcgga gccaggcgcg
     2461 cgacgaccgc gtcgtggccg gcggcctccg ccatgctgcg ggcggtcttg ccggtcaccc
     2521 agcctcccca ctcgaacccg accacgaggg tcagcacgac cacgaccacc caggaccaga
     2581 acatcgcggt cttggtcggc cgcacgtctc tccatcgctc cgcgagactg cgcttccggg
     2641 gcatcgtctc catcgcattc ctcctaccgg acggtcgaga cttcggctga catcgtaccg
     2701 ggggcggatg aaggcccggt gagagccgga tgagaatgcg cccgggagcg cgggcgcggg
     2761 cctcgcggcc tagccgccct tcaggcgggg gtcgagcagg tcgcgcagcg cgtcgccgag
     2821 catgttgaag ccgaagaccg cgagcgacag cgccaccgcg ggccagatcg cgagccacgg
     2881 ggcacggtac atgtggtcga ggccggcgag cgagagcatc ccgccccagg tcgggtaggg
     2941 tggcggcacc ccgaggccga ggaacgagag gctcgcctcg gtgaggatga cggtggagag
     3001 cccggtggtc gcgaccacga tgatggtcgg caccacgttc ggcaggatgt gggtcagcgt
     3061 ggtacggaga tgccccgcgc cgagggcccg cgcgctctcc acgaaggagt gctccttgac
     3121 gagcagggcg acgccccgaa ccacccgcga gttctggatc ccgaaggcga tgccgagcac
     3181 gacggtgatg ttccacacgc tcgggccgag cagcgacatg atcgagagca gcaggagcag
     3241 cggcgggaag gccatccacg cgtccacccc gcgctgcacc agcaggtcga agcgaccgcc
     3301 caggtagccc gagaccaccc cgatcgcggt ggcgagcacg ctgccgatgg cgatcgcccc
     3361 gaagcccacg tagagcgaga tgcgcgcccc gtagatgagc cgggtgagca ggtcgcgccc
     3421 gagctcgtcg gtgccgagcc agtgcgcggc gctcgcgacg atgaagcgct cccgcaggct
     3481 ggtctgggcg aagccgtagg gcgcgatcca gtcggccagc accgcggcgg ccagcatcac
     3541 gagcacgatc gcgccgccca ccgcgcccag cggctttccg gtgagcatgc gcacccacat
     3601 gtcgagccac cacgggcgcg cccgcgggcc gacccgcgcg ggcaggctca gcgcggccgc
     3661 gccgcccgcc gcctcggggg cgagggcggg ccggcgcatg ccgaacgcgc gggtccagga
     3721 ggggcgcatg cgcctagtac cggatgcgcg ggtccaggaa ggcgtacgag aggtcgatga
     3781 ggaggttcag ccccaccacc atcgcggcga tgatcaggtt caccgactgc acgaccggat
     3841 agtcgaggcg ggtcagggcc tcgaagaaga agaggccgac gccgggcagg cggaagatcg
     3901 attcgatgat gacggagccg ccgatgtaga gcggcatctg caggccgatc aggctgatca
     3961 ccgggatcat cgcgttcttg agcgcgtggc gcaggatgat cgggcgctcg gccagcccct
     4021 tggaccacgc ggtgcggatg tagtcctgcc ggagcacgtc gagcagcgcg gagcgcatga
     4081 tgcgcatgac cgggccggcc cgggtcaccc cgaagaccag cgcgggcacc agcatgatct
     4141 tgaggttctc gagggggtcg acggtgaagg ccacgtaggt ccagaccggc gtccacttga
     4201 agtagatcga cggcagcacc accaccagca ccgcgaggcc gaagtagggg acggagagcg
     4261 cgaggatggc gagcacccgg cccaggtagt ccgccgcgct gtcctgccgc gcggcggaca
     4321 cgatgccgat gaccaccccc agcaccaccg agaccaggat cgtgagcagg gccagctcga
     4381 gggtgaccgg gaaccgccgg ccgaactcgt cgaggatggg ctggcgcgtc cagtaggagt
     4441 ggccgaagtc gccccgcagg acgatgccgc cgatccagtc gaggtactgg gtgtggacgg
     4501 gccggtcgag gccgaggtcg tggcggaggg ccgcgagcgt gggggcgtag gcgtgcccct
     4561 cgaccatgcg ggtggccacg tcgccgggca tgaagcgcat gagcgcgaag accatcacgc
     4621 tcaccaggaa cagcgtcggc accgccagca gcacgcgccg gaggacgtac cgcttcaccg
     4681 cgcgcccctc acccccgccg cgtcacttgt cgagccaggt gtagaggagc cgcatcccga
     4741 ggccgtagcc gtcgtggtgc cggaagccct tcacgtacgg cgggtgcgcc acgtactgcg
     4801 gccactgcgg cacgtagacg tagtaggcct tgtcggccag gtagcgctgg atgtcgtcga
     4861 cgatctgctt ccgcttcctg ggatccatct cgcgccgctg ggccaccagc atcttggtca
     4921 gctcgccgtc ggccacgtgg ctctggttgg tgggcagctc cgggtagaag cgcccgaaga
     4981 ggaagtcgtc cacctcggtg aacggcgtcg acgggcccat cgcggccttg tcgtacttgc
     5041 cgagcgcggt ggtggagatg tacttgccgt actcctccgg cttcagctcg acggtgatcc
     5101 cgatcttgcc caggtcctcc gcggccagct cgtagtagtt gcgccacggc accacgtagc
     5161 ccggccagtg gtagatcggc atggtgaagc ccttggcgta gcccgcctcc gcgagcagct
     5221 tcttggcctc ggccgggtcg tagcccacca cgtacttggc cttggccggc tccatcttgg
     5281 ccgcgtcgag cttccagtcg gtgagcgcgc agggcaccgg ccccgcgtcc acgcagccct
     5341 ctccgaacag cagcgcgtcg ttccacgact tgcggtcgat ggcgagcgag accgcgcggc
     5401 gcacccgcac gtcgttgaac ggaggctggt cggtgcgcat gtagatgaag ccctggccga
     5461 tgaccttgta aggggtgatc gacatctcgg ggctggtctg cttgagcgag cgcgcctcct
     5521 ccgggctgat ccagccccag acgtgcggca gctccgcctt gcccgcccgc agcaccgcca
     5581 ggcgcgccgc cgcgtcgggg gtgatgtcga cggtgacccc gtcgaggtag gggaagccct
     5641 tcatgaaata ggtcgggttg cgctcgaaga ccatgcgcac gcccttctcg tacgacttga
     5701 gcacgaaggg cccggtgccg atgaccgcgt ccgggttgtt gaagtccttg aacttctcct
     5761 ccgcctcgcg tggca
//
LOCUS       DATMCV010000581         8269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_165775, whole genome shotgun sequence.
ACCESSION   DATMCV010000581 DATMCV010000000
VERSION     DATMCV010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165775"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VNU03_13420"
     CDS             complement(<1..958)
                     /locus_tag="VNU03_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWP75516.1"
                     /translation="MRVQLLSVNDVYVLEPVAGRGGLARVATLVRALERETPNTLFAL
                     AGDTLSPSLLSTLRRGAQMIEGWNALGLGVAAFGNHEFDFGPAVLAERIGESRFPWLA
                     TNVLDRATGRPLGGARAWLRRDFDGVQVGVIGLTTPDAARTSNGGPGVRFDDPLEAAR
                     GALAALGAVDLAVAVTHLPVAQDRALVAALPIDAILGGHDHDPMLLEEGRAVIVKAGS
                     DAVNVGQVEYELRCGAVLSRRQRLIPVDERIAEAPDVADLVRRQAALLARELDTTVGR
                     TPVPLDARESLVRREETPLGRFFAELMRERVGAQVGLLNGGAI"
     gene            complement(1052..1975)
                     /locus_tag="VNU03_13425"
     CDS             complement(1052..1975)
                     /locus_tag="VNU03_13425"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWP75517.1"
                     /translation="MKRLLRWIRTRLPRDRAKRTLILAIVTSALFHLLFFLPFITIPG
                     FLQSPTYVKRGEPLLVDIAPERPEEKAPRGNPSQPVSPPESREQPSPPAAKAPAPRAP
                     EAPRAPAPRPTPPSPPRVARTEPPAPEAMVKTTEPSRPAPETPPSQATPSESAPAPSQ
                     SPQVAVARPPTSIWRQPGGGGGMQGGGRGGVEGQPIPLDTPEPKYQDYFNKVRERIKA
                     NWVYPRQAGERNIEGELVIEFHIAKDGRLEFIELRDSSGTRILDDAALTAVKLAQPFP
                     PVPDDIAKRTLAINGQFRYQIVSGLVNQFLR"
     gene            2190..3005
                     /locus_tag="VNU03_13430"
     CDS             2190..3005
                     /locus_tag="VNU03_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWP75518.1"
                     /translation="MRTLAKILVAFAGLLLAAGPAHGQPGPLAFGVLNQQSPALTAER
                     WNPILHYVGTAAGIPLQLRMGRTVQETDAMMGRGDFDFVFTNHNFQSDFDAVPFKVLA
                     RWAGEPIRAVIAVPADSPIHHLHELGGKRVSFPSSDAFAAYAVPLVALKRAGVQVEEV
                     FGGNQEGTLAQLKAKRVEAAAVNSRFLAQYAERERVEFREIYVSDGFPDLAVIAHPRV
                     PAATVERVRRALLGMAADPAAAPILTQTKSKGFEPATDRDYEGVRRIYRLIGQ"
     gene            3002..5398
                     /locus_tag="VNU03_13435"
     CDS             3002..5398
                     /locus_tag="VNU03_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP75519.1"
                     /translation="MRNSLRTLLLGLSSVAIVSVTLGGLAVNLRERSTAAQVRLAEQT
                     ERLAVAAAPLLLDSLVVGDLARAEQTVRNLNTDLVWSQVRLYESDGRRVIFDASPQKV
                     RRSHAPRWLKQLVPVTLAEHRVVIVAPPVTYGVLAVTPSVESLENELWTEVRTMIAVT
                     GVLLVTLLGLTHVILVVGLRPVRALAVSATRLGHGDLTARMPETRLSEMRPTVRAFNS
                     MAASLEQALHEARTRQTRLESLVEISGELSRMQPLDSLLGRIAEACGRIVKTDWVGFR
                     LVDGDELVVAGTLDVGSDMPVRPRLKIGESLAGQVAATGRPLLLRDPASHPSMLPEHS
                     AAMRRLGYQGLLTVPARVGDRVVGVLSFLTRREEGFTTEDLAIASAFASHAAIALENS
                     RLLHESRRAYEELEHTQGQLEQAQKMDAIGRLAGGVAHDFNNLLTVILGRTDILLHQL
                     KAEDPLRRGIELIQRTAGRAAELTKQLLAFSRKQVLEAVVLDLGAVTVDMKEMLARLI
                     GEDIALVTNNAAGLGHVKADRGQIEQVVMNLAINARDAMPKGGHLIVETENVDLDAEY
                     VRRNMGARPGPHVMLAVTDSGVGISREHQRHIFEPFFTTKEQGKGTGLGLATVYGIVK
                     QSGGYIEVDSEPGRGTTFRIYLPRVDAAAPVADRSIRSAAPAGGTETILLVEDEEGVR
                     ELARDILRSSGYTVIEARNGAEALLLCERHQGPLDLLLTDVVMPRMSGRELAERLGPL
                     RPDLSVLYMSGYTDDAVIRHGVLGADTAFLQKPFTPVALVQRVRETLDLARREPTPVA
                     "
     gene            complement(5403..6419)
                     /gene="aroF"
                     /locus_tag="VNU03_13440"
     CDS             complement(5403..6419)
                     /gene="aroF"
                     /locus_tag="VNU03_13440"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWP75520.1"
                     /translation="MIIVLKSGINDADVDEVCRRITEMGYGPHVIRGEFKTIVAAVGE
                     ERGRPDLRLLEAMETVESVMPVQQPFKLASREVRRDSTEVRINGVTVGGRDVVVMAGP
                     CSVESQAQVLEVADAVKGSGAKILRGGAYKPRTSPYAFQGLKEEGLRYLAEARKRTGL
                     PVVTEVLETESVEVVAEYSDILQIGARNIQNFTLLRRVGEIGKPVLLKRGMATSIQEF
                     LLSAEYILSAGNPNVILCERGIRTFETATRFTLDLNAVPVLKKLCHLPVVVDPSHGTG
                     HWDLVAPMAKGAVACGADGLIIEVHPRPEEALSDGPQSLKPSKFAQLMRELRPVAEAV
                     GRGL"
     gene            complement(6416..7432)
                     /gene="aroF"
                     /locus_tag="VNU03_13445"
     CDS             complement(6416..7432)
                     /gene="aroF"
                     /locus_tag="VNU03_13445"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWP75521.1"
                     /translation="MIIVLKPGSSEADIEDVSRRVRELGFKTHLSRGEVRTIIGIVGD
                     DRAKEQLLALQSLDSVENVVRILQPFKLASREAHPDNTQFKVHGVPVGGDQIVVMAGP
                     CSVESQPQLNAVAESVKAAGAHVLRGGAFKPRTSPYAFQGLEEDGLTLLREASKRTGL
                     PVVTEVMEPDKVEVVAEHADILQIGARNVQNFSLLKRVAECGKPVLLKRGMSTSIQEW
                     LLSAEYVLAGGNPNVILCERGIRTFETSTRYTLDLNAIPVVKKLSHLPIFVDPSHGTG
                     HWEYVSAMAKAGLAAGADGLIIEVHNNPAEALSDGPQSLKPNKFAQLMAELRPLAQVL
                     GRTL"
     gene            complement(7800..>8269)
                     /locus_tag="VNU03_13450"
     CDS             complement(7800..>8269)
                     /locus_tag="VNU03_13450"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWP75522.1"
                     /translation="FYSTIGLTLGSVVAFWIGRWLGAHYVKNLVSAETWNRLGFIVEA
                     EGAVLCFIIFLIPGLPKDIVCYIFGISPMPLWVFALVSGLGRIPGTWVLSAQGAHTAA
                     GDYMQVILVSAFAVAAALPLYYYRHRIVTWFHGRSEARAKDANGGLDREEPGP"
BASE COUNT         1193 a         3004 c         2856 g         1216 t
ORIGIN      
        1 ggatggcgcc gccgttgagc agccccacct gggccccgac ccgctcccgc atcagctccg
       61 cgaagaaccg cccgagcggc gtctcctcgc ggcggacgag gctctcccgc gcgtcgagcg
      121 ggacgggggt gcggcccacc gtcgtgtcga gctcccgcgc gagcagcgcg gcctggcggc
      181 gcacgagatc cgccacgtct ggggcctcgg cgatccgctc gtcgaccggg atgagacgct
      241 ggcgccggct gagcaccgcg ccgcagcgca gctcgtactc gacctggccg acgttgaccg
      301 cgtccgagcc ggccttgacg atgaccgcgc ggccctcctc gaggagcatc ggatcgtgat
      361 cgtgaccgcc caggatcgcg tcgatcggga gcgccgcgac cagcgcgcgg tcctgcgcga
      421 ccggcaggtg ggtcaccgcc accgccaggt cgaccgcgcc gagggcggcg agggcgccgc
      481 gcgccgcctc gagcggatcg tcgaagcgca ccccgggccc gccgttggag gtccgggccg
      541 cgtcgggcgt ggtgaggccg atcactccca cctgcactcc gtcgaagtca cgacggagcc
      601 acgcgcgcgc gcccccgagc ggccggccgg tcgcgcggtc gaggacgttg gtggcgagcc
      661 acgggaagcg cgactcgccg atgcgctcgg ccagcaccgc ggggccgaaa tcgaactcgt
      721 ggttgccgaa ggcggcgacg ccgagcccga gcgcgttcca gccctcgatc atctgcgccc
      781 cgcgacgaag ggtcgagagc agcgacggcg acaaggtgtc acccgccagc gcgaagagcg
      841 tgttcggcgt ctcgcgctcg agggcccgca cgagcgtggc cacgcgcgcg aggccgcccc
      901 ggcccgcgac cggctcgagc acgtagacgt cgttcaccga gaggagctgg acgcgcaccg
      961 gcggccggtc cgcgcagcgc gtctcggcgc accccgcgac gatcggcagc aggagaagcc
     1021 atgcggcgag cgagcgaagg ggcaggatcg tctaccggag gaactggttc acgagaccgc
     1081 tcacgatctg gtaccggaac tggccgttga tcgcgagcgt gcgcttggcg atgtcgtcgg
     1141 gcaccggcgg gaacggctgc gccagcttca ccgcggtcag cgccgcgtcg tcgaggatcc
     1201 gggtgccgga ggagtcccgc agctcgatga actcgagccg cccgtccttg gcgatgtgga
     1261 actcgatgac cagctcaccc tcgatgttcc gctcgccggc ctggcgcggg tatacccagt
     1321 tcgccttgat ccgctcgcgg accttgttga agtagtcctg gtacttcggc tccggtgtgt
     1381 cgagcgggat cggttgccct tcgactccac cgcgcccgcc gccctgcatg ccgcctcccc
     1441 cgcccggctg gcgccagatg ctggtcggcg gccgggcgac cgcgacctgc ggcgactggc
     1501 tcggggccgg cgcggactcg ctaggcgtcg cttgcgacgg cggtgtctcg ggcgccgggc
     1561 ggctgggctc ggtcgtcttc accatcgcct ccggcgcggg cggctcggtg cgcgcgaccc
     1621 gcggtggcga cggcggcgtc gggcgcggcg cgggcgcgcg cggagcctcg ggcgcgcgcg
     1681 gggccggggc cttggcggcg gggggcgagg gttgctcccg cgactcgggt ggggacaccg
     1741 gctgcgatgg attgccccgc ggcgctttct cttccggcct ctcgggcgcg atgtcgacga
     1801 ggagcggctc gccgcgcttg acgtaggtgg gggactgcaa gaagcccggg atcgtgatga
     1861 acggcaggaa gaacagcagg tggaagagag ccgacgtgac gatggcgagg atcagggtgc
     1921 gcttcgcacg atcccgcgga agcctggtcc ggatccaccg tagcaggcgc ttcacgggag
     1981 caattatggc acgccgtccc agccatcgcc gggtggcccc gaaccccccg gtctcacgcc
     2041 tcttctgacg aaatgtgtcc gtctggtgac acccagcggc cctcggcagc ttacgcttac
     2101 ggcgccacac aagctttctc cactatttca catatttgca gcctggcttc acttggcacg
     2161 gccctggaaa gcacttggcg cccaacatca tgcgaaccct ggcgaaaatt ctcgtcgcct
     2221 tcgccggcct gctcctcgcg gccgggcccg cccatgggca gccgggtccg ctcgccttcg
     2281 gcgtgctcaa tcagcagagc ccggcgctca ccgcggagcg gtggaatccg atcctgcact
     2341 acgtcggcac ggccgccggc atcccgctcc agctccggat gggccgcacc gtccaggaga
     2401 cggacgcgat gatgggacgg ggcgacttcg acttcgtgtt caccaaccac aacttccagt
     2461 ccgacttcga cgcggtgccg ttcaaggtcc tggctcgctg ggcgggcgag cccatccgcg
     2521 cggtgatcgc ggtgcccgcc gacagcccca tccaccacct gcacgagctc ggcggcaagc
     2581 gcgtgtcctt cccctcctcc gacgcgttcg cggcctacgc ggtgccactc gtcgcgctca
     2641 agcgcgcggg agtccaggtc gaggaggtct tcggcggcaa tcaggaaggc acgctcgcgc
     2701 agctcaaggc caagcgcgtc gaggcggccg cggtcaactc gcgcttcctc gcccagtacg
     2761 ccgagcgcga gcgcgtggag ttccgcgaga tctacgtctc ggatggattc ccggacctcg
     2821 cggtcatcgc ccatccccgg gtgcccgccg ccaccgtcga gcgggtgcgg cgcgcgctgc
     2881 tcgggatggc cgccgacccc gccgcggcgc cgatcctgac ccagaccaag tccaagggct
     2941 tcgagccggc gaccgatcgc gactacgagg gcgtccgccg catctaccgc ttgatcggcc
     3001 agtgaggaac agcctccgca ccctgctcct cggactcagc tcggtggcga tcgtcagcgt
     3061 caccctgggc ggcctcgcgg tcaacctgcg tgagcgcagc accgcggccc aggtgcgcct
     3121 cgccgagcag accgagcggc tcgccgtcgc cgcggccccg ctgctgctcg attcgctggt
     3181 ggtgggcgat ctcgcgcgcg ccgagcagac cgtgcgcaat ctcaacaccg atctggtgtg
     3241 gagccaggtc cgtctctacg agagcgacgg gcggcgggtc atcttcgacg cgtcccccca
     3301 gaaggtccgt cggtcccacg ccccgcgctg gctgaagcag ctcgtgccgg tgacgctcgc
     3361 ggagcaccgg gtcgtcatcg tcgcgccccc ggtgacctac ggggtgctgg cggtgacccc
     3421 ctcggtggag agcctcgaga acgagctgtg gaccgaggtc cgcaccatga tcgcggtgac
     3481 cggggtgctc ctggtcaccc tcctcggcct cacccacgtg atcctggtgg tgggcctgcg
     3541 tccggtgcgg gccctcgccg tgagcgcgac gcgtctcggg cacggcgatc tgaccgcgcg
     3601 gatgccggag accaggctga gcgagatgcg gcccaccgtc cgcgccttca actcgatggc
     3661 ggccagcctc gagcaggcac tgcacgaggc ccgcacccgc cagacgcgac tcgagagcct
     3721 ggtcgagatc agcggcgagc tgtcccggat gcagccgctc gactccctcc tggggcggat
     3781 cgcggaggct tgcgggcgca tcgtgaagac cgactgggtg ggcttccggc tcgtggacgg
     3841 cgacgagctc gtggtggcgg gcacgctcga cgtgggctcg gacatgccgg tgcggccccg
     3901 cctgaagatc ggcgagagcc tggccgggca ggtcgcggcc accggccgcc cgctgctcct
     3961 gcgcgacccg gccagccatc ccagcatgct gcccgagcac tcggcggcca tgcggcggct
     4021 gggctaccag ggactcctga ccgtcccggc ccgggtcggg gaccgcgtcg tcggcgtgct
     4081 cagcttcctg acccgccggg aggagggctt caccaccgag gacctcgcca tcgcgagcgc
     4141 cttcgcgtct cacgcggcga tcgccctcga gaacagccgc ctgctccacg agtcccggcg
     4201 ggcgtacgag gagctggagc acacccaggg tcagctcgag caggcccaga agatggatgc
     4261 gatcggccgg ctcgcgggcg gggtcgcgca cgacttcaac aacctcctga ccgtcatcct
     4321 cgggcgcacc gacatcctgc tccaccagct gaaggcggag gatcccctgc ggcgcggcat
     4381 cgagctgatc cagcgcaccg ccggccgcgc cgccgagctg accaagcagc tcctcgcgtt
     4441 cagtcgcaag caggtgctcg aggcggtcgt cctcgacctc ggcgcggtga ccgtcgacat
     4501 gaaggagatg ctcgcgcggc tcatcggcga ggacatcgcg ctcgtgacca acaacgcggc
     4561 cggcctcggc cacgtgaagg ccgaccgcgg gcagatcgag caggtggtga tgaacctggc
     4621 gatcaacgcg cgcgacgcca tgcccaaggg cggccacctc atcgtggaga ccgagaacgt
     4681 cgacctcgac gcggagtacg tgcgccgcaa catgggcgcg cgcccgggcc cgcacgtgat
     4741 gctcgcggtg accgacagcg gggtgggcat ctcccgcgag caccagcgcc acatcttcga
     4801 gccgttcttc accaccaagg agcagggcaa ggggacgggg ctcgggctgg ccaccgtcta
     4861 cggcatcgtc aagcagagcg gcggctacat cgaggtcgac agcgagccgg gccgcggcac
     4921 caccttccgc atctacctgc cgcgcgtcga cgcggcggcg ccggtcgcgg accggtcgat
     4981 ccgctccgcc gcgccggcgg gcggcaccga gaccatcctg ctcgtcgagg acgaggaggg
     5041 cgtgcgggag ctcgcgcgcg acatcctgcg gtccagcggc tacaccgtga tcgaggcgcg
     5101 caacggcgcc gaggccctgc tcctctgcga gcgccatcag ggcccgctcg atctcctgct
     5161 caccgacgtg gtcatgccgc gcatgagcgg gcgcgagctc gccgagcgcc tggggccgct
     5221 gcgccccgac ttgagcgtcc tgtacatgtc gggctacacc gacgacgcgg tcatccgcca
     5281 cggcgtcctc ggcgccgaca ccgcgtttct gcagaagccg ttcacgccgg tggccctggt
     5341 ccagcgcgtg cgcgagacgc tcgacctggc ccggcgcgag ccgacccccg tcgcctgacc
     5401 gcctagagcc cgcgccccac cgcctccgcc accggccgca gctcgcgcat gagctgggcg
     5461 aacttcgagg gcttgagcga ctgcggcccg tcggacagcg cctcctccgg ccgcgggtgc
     5521 acctcgatga tcagcccgtc cgccccgcac gccaccgccc ccttggccat cggggcgacc
     5581 aggtcccagt gcccggtgcc gtgcgaggga tccaccacga ccgggaggtg gcagagcttc
     5641 ttcagcaccg gcaccgcgtt gaggtcgagc gtgaagcggg tggcggtctc gaaggtgcga
     5701 atgccgcgct cgcagaggat cacgttcgga ttgcccgcgg agaggatgta ctcggccgag
     5761 agcaggaact cctggatgct ggtggccatg ccgcgcttga ggagcaccgg cttgccgatc
     5821 tcccccaccc ggcgcagcag cgtgaagttc tggatgttcc gcgccccgat ctggaggatg
     5881 tcgctgtact ccgccaccac ctccacgctc tccgtctcga ggacctcggt gaccaccggc
     5941 agcccggtgc gcttgcgcgc ctccgccagg tagcgcagcc cctcctcctt caggccctgg
     6001 aaggcgtagg gcgaggtgcg cggcttgtag gccccgcccc gcaggatctt ggcgccggat
     6061 cccttgaccg cgtcggccac ctcgagcacc tgcgcctgcg actccaccga gcagggcccc
     6121 gccatcacca ccacgtcgcg cccgcccacc gtgaccccgt tgatgcgcac ctcggtcgag
     6181 tcgcggcgta cctcgcggct ggccagcttg aacggctgct gcaccggcat gaccgactcg
     6241 accgtctcca tcgcctcgag gagacggagg tcgggccggc cccgctcctc gcccaccgcc
     6301 gcgacgatgg tcttgaactc gccgcggatc acgtgcgggc cgtagcccat ctcggtgatg
     6361 cggcgacaca cctcgtccac gtcggcgtcg ttgatcccgg acttgagcac gatgatcata
     6421 gggtcctccc cagcacctgg gccagcggcc ggagctcggc catgagctgc gcgaacttgt
     6481 tcggcttgag cgactgcggg ccgtcggaca gggcctcggc cgggttgttg tgcacctcga
     6541 tgatgaggcc gtcggccccg gccgccagac cggccttggc catcgccgac acgtactccc
     6601 agtgcccggt gccgtggctc gggtccacga agatcggcag gtgggagagc ttcttcacca
     6661 ccgggatcgc gttgaggtcg agggtgtagc gggtggaggt ctcgaaggtg cggatgcccc
     6721 gctcgcagag gatcacgttc gggttcccgc ccgccagcac gtactcggcg gaaagcagcc
     6781 actcctggat cgaggtcgac atgccccgct tgagcagcac cggcttgccg cactcggcca
     6841 cccgcttgag cagggagaag ttctgcacgt tgcgggcccc gatctggagg atgtccgcgt
     6901 gctcggccac cacctcgacc ttgtccggct ccatgacctc ggtcaccacc ggcaggccgg
     6961 tgcgcttgct cgcctcccgc agcagggtga ggccgtcctc ctcgagcccc tggaaggcgt
     7021 agggcgaggt gcgcggcttg aaggcgccgc cccggagcac gtgcgcgccg gcggccttga
     7081 cgctctccgc caccgcgttg agctggggct gcgactccac ggagcacggc ccggccatca
     7141 ccacgatctg gtcgccgccg accgggaccc cgtggacctt gaactgggtg ttgtcgggat
     7201 gggcctcacg gctcgcgagc ttgaagggct ggaggatccg caccacgttc tccaccgagt
     7261 cgagcgactg cagcgcgagg agctgctcct tggcccggtc gtcgccgacg atcccgatga
     7321 tggtgcgcac ctccccgcgg gagaggtggg tcttgaaccc gagctcccgc acccgtcgtg
     7381 agacgtcctc gatgtcggcc tcgctcgatc ccggcttcag cacgatgatc atgtgtgcgc
     7441 ccccttttcc gtctcgtctt ccgtttgtgg tgcccgtcgc tgccgtttac ttcttcaagc
     7501 tcttcagctg cgggggcccc gaaatggccc ccgcactccc ccaggtcgcg gacacccgtt
     7561 tacgacggtc ggttggccgc cgtagaaaac aaaaacgccg cgggtcggtt gcccgcggcg
     7621 tctatcggtt cctcgccacg ggctggttcg tcgcccgtgg ctccttgcga tcagctcatg
     7681 ggagccaggc ggcgaacggt gggaaagtaa aaatacgacc gccacgctcc gtggctgctg
     7741 gtcgtcgttc ccatcgtgct caccggctcg attccctcca ggctcaaagt ggggcgagtt
     7801 tacgggcccg gctcctccct gtcaagccct ccgttggcgt ccttcgcccg ggcctcgctc
     7861 cgcccgtgga accaggtcac gatacggtga cggtagtagt agagcggcag ggccgccgcc
     7921 accgcgaagg ccgagaccag gatcacctgc atgtagtcgc cggccgcggt gtgggctccc
     7981 tgggcggaga gcacccaggt gcccgggatc cggccgagac ccgagaccag ggcgaagacc
     8041 cagaggggca tcgggctgat cccgaagata tagcagacga tgtccttcgg caggccgggg
     8101 atcaggaaga tgatgaagca gagcaccgcc ccttcggcct ccacgatgaa gccgagccgg
     8161 ttccacgtct cggcgctgac caggttcttc acgtagtgcg caccgagcca ccgtccgatc
     8221 cagaaggcca ccaccgagcc cagcgtgaga ccgatggtcg agtagaaca
//
LOCUS       DATMCV010000582         3023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_365638, whole genome shotgun sequence.
ACCESSION   DATMCV010000582 DATMCV010000000
VERSION     DATMCV010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365638"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VNU03_13455"
     CDS             <1..636
                     /locus_tag="VNU03_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75523.1"
                     /translation="RDTGVGIAPEMLPRVFDLFTQGERGPDRGAGGLGLGLTLVHRIA
                     ELHAGRVEAASEGPGHGSTFTVSLPAMAAPPAAVSAPVAAARNGAPRRILVVEDNGDA
                     REMLRHLLRLAGHEVLEAVDGPGGLEATLRARPDVALVDVGLPGFDGYELARRVRATG
                     DSSIYLVALTGYGQPDDRRQAMESGFDAHLVKPVNPEALLAAIHAGAPGGS"
     gene            639..1496
                     /locus_tag="VNU03_13460"
     CDS             639..1496
                     /locus_tag="VNU03_13460"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP75524.1"
                     /translation="MTIAVTRLTPCFAARVDGADITRPLDDTTWTAIRAAFEEHSVLL
                     FRGQPLDDDTQIAFSLRFGALEVTRSMNPAAGTPFARQSNLDIKTGAVIPPDDRRMVY
                     QLANMFWHSDSSFKPVPSLCSLLSGRIVPPAGGATELASARAAYPSLPEPLRRRAETL
                     TVVHDFSWSRDQVRPGFFTAEERAVYPPVRHPLVRVNPVNGQPALFLGAHASHVEGLP
                     IDEGRALLRTLLDHVTQPRFVYRHEWQEGDLIVWDNRCVLHRATPYDTTSHKRLMQRT
                     TVSGDPAER"
     gene            1493..2734
                     /locus_tag="VNU03_13465"
     CDS             1493..2734
                     /locus_tag="VNU03_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP75525.1"
                     /translation="MIDYKHLVQDDRIHASLYTDPQVFDDEMERIFHRGWVFVGHSSE
                     IPHPGDYVTRVVGTEPVIMVRAGDGEISILINRCRHRGTMVCPADRGHARTLTCPYHG
                     WTYDLRGELLGVPYPGGYAGFDKSAHGLPRAPRVSAYRGFVFASLAADGISLAEHLGP
                     ATRLIDRSCDLSPVGEVELTAGWVRHRCAANWKMLPENDSDGYHLGFVHMALFRTIQS
                     QYRRVVGDERSIKAVVRDWGRGHIEIDWSPGYEGLFEWLGNASGSAVERFVAEIERRD
                     GPEVARRRFREGPAHALIFPNLFLGETNIAIVQPVSVEECVHWHTPMFWTGVPEWNGR
                     LLRMAEAGMGPASFLMPDDLIIASRNQLGLHARTSPWLLLGRGLNRETTDADGRIVSH
                     VTDETTNRGFWRHLRSVMSEA"
     gene            2731..>3023
                     /locus_tag="VNU03_13470"
     CDS             2731..>3023
                     /locus_tag="VNU03_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HWP75526.1"
                     /translation="MSTAEADHRAVAAFLYREARLADEARYAEWLALWTDDGVYWVPA
                     TTDPDANSERHLSHIYDNRARIETRVKLLQTGVRYSQEPASRMRRLISNIEVES"
BASE COUNT          414 a         1165 c         1032 g          412 t
ORIGIN      
        1 cgcgataccg gcgtcgggat cgcgcccgag atgctgccgc gcgtcttcga cctgttcacc
       61 cagggcgagc gcgggcccga ccgcggggcg ggcggcctcg gcctgggcct caccctggtc
      121 caccgcatcg cggagctgca cgcgggccgc gtcgaggcgg ccagcgaggg gcccggccac
      181 ggcagcacgt tcacggtgtc cctgcccgcc atggccgccc cgcccgccgc cgtctccgcc
      241 ccggtggcgg cggcccggaa cggcgcgcct cgccgcatcc tcgtggtcga ggacaacggc
      301 gacgcgcgcg agatgctgcg ccacctgctg aggctcgccg ggcacgaggt gctggaggcg
      361 gtggacggcc ccggcggcct cgaggcgacc ctgcgcgcgc gcccagacgt cgccctggtc
      421 gacgtggggc tccccggctt cgacggctac gagctggccc gccgggtccg ggccaccggc
      481 gactcctcca tttacctcgt ggccctgacc ggctatggtc agcccgacga ccggcgccag
      541 gccatggagt cgggcttcga cgcccacctg gtgaagccgg tcaatccgga ggcgctgctg
      601 gccgccatcc acgccggggc gccgggaggg agctgagcat gacgatcgcg gtgacccggc
      661 tcaccccctg cttcgcggcg cgcgtcgacg gggccgacat cacccgcccg ctcgacgaca
      721 cgacctggac cgcgatccgc gccgccttcg aggagcactc ggtgctgctc ttccgcggcc
      781 agccgctcga cgacgacacc cagatcgcgt tcagccttcg cttcggcgcg ctcgaggtca
      841 cccggagcat gaacccggcc gcgggcacgc ccttcgcgcg ccagtccaac ctggacatca
      901 agaccggggc cgtcatcccg cccgacgacc ggcggatggt ctaccagctc gccaacatgt
      961 tctggcacag cgacagctcg ttcaagccgg tcccctcgct ctgctcgctg ctctccggcc
     1021 gcatcgtgcc gcccgcgggt ggggccaccg agctcgcgag cgcgcgcgcc gcctacccct
     1081 cgctgccgga gccgctgcgg cgccgggcgg agaccctgac ggtggtgcac gacttctcgt
     1141 ggtcgcgcga ccaggtgcgg cccggcttct tcaccgccga ggagcgcgcg gtgtacccgc
     1201 cggtgcgcca cccgctggtg cgggtgaacc cggtgaacgg gcaacccgcg ctcttcctcg
     1261 gcgcgcacgc ctcccacgtg gaggggctgc cgatcgacga ggggcgcgcg ctcctgcgca
     1321 ccctgctcga ccacgtcacc cagccccggt tcgtctaccg gcacgagtgg caggaaggcg
     1381 acctgatcgt ctgggacaac cgctgcgtgc tccatcgcgc gacgccgtac gacaccacca
     1441 gccacaagcg gctcatgcag cgcaccaccg tctccgggga cccggccgag cgatgatcga
     1501 ctacaagcac ctcgtgcagg acgatcggat ccacgcgagc ctctacaccg acccgcaggt
     1561 gttcgacgac gagatggagc gcatcttcca ccgcggctgg gtcttcgtcg gccactcgag
     1621 cgagatcccg cacccggggg actacgtcac ccgcgtcgtc ggcaccgagc cggtcatcat
     1681 ggtgcgggcc ggcgatggcg agatctcgat cctgatcaat cgctgccgcc accggggcac
     1741 catggtctgc ccggccgacc gcggccacgc ccggacgctg acctgtccct accacggctg
     1801 gacctacgac ctgcggggcg agctgctcgg ggtgccgtac ccgggcggct acgccggctt
     1861 cgacaagagc gcccacggcc tgccgcgcgc gccgcgggtg tccgcctatc gcggcttcgt
     1921 gttcgcgagc ctcgcggccg acggcatctc gctcgccgag cacctgggcc cggccacccg
     1981 gctcatcgac cgctcctgcg acctctcgcc ggtgggcgag gtcgagctga ccgcgggctg
     2041 ggtccggcac cgctgcgcgg ccaactggaa gatgctcccc gagaacgaca gcgacggcta
     2101 tcacctcggc ttcgtgcaca tggcgctctt caggacgatc cagtcgcagt accggcgggt
     2161 cgtcggggac gagcgctcga tcaaggcggt ggtgcgggac tggggccgcg gccacatcga
     2221 gatcgactgg tcgcccggct acgagggcct cttcgagtgg ctcgggaatg cgtccggctc
     2281 cgcggtggag cggttcgtgg ccgagatcga gcggcgcgac gggcccgagg tggcgcggcg
     2341 acggttccgc gagggccccg cgcacgccct catcttcccc aacctcttcc tcggcgagac
     2401 caacatcgcg atcgtccagc cggtctcggt ggaggagtgc gtgcactggc acaccccgat
     2461 gttctggacc ggcgtgcccg agtggaacgg ccggctgctc cgcatggccg aggccggcat
     2521 gggccccgcg tcgttcctga tgccggacga cctgatcatc gcgagccgca accagctcgg
     2581 cctgcacgcg cgcacgagcc cgtggctgct cctcggccgc ggcctgaacc gggagaccac
     2641 cgacgcggac ggccgcatcg tctcccacgt caccgacgag acgaccaacc gcggcttctg
     2701 gcggcacctc cgctccgtga tgtcggaggc gtgagcaccg ccgaggccga tcaccgcgcg
     2761 gtcgccgcct tcctctaccg ggaggcgcgg ctggccgacg aggcgcgcta cgcggagtgg
     2821 ctcgcgctct ggaccgacga cggggtgtac tgggtgcccg cgactaccga ccccgacgcg
     2881 aactcggagc gtcacctctc ccacatctac gacaaccgcg cccgcatcga gactcgcgtc
     2941 aagctgctgc agaccggcgt gcgctacagc caggagcccg cctcccgcat gcggcgcctc
     3001 atctccaaca tcgaggtgga atc
//
LOCUS       DATMCV010000583         2566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_433239, whole genome shotgun sequence.
ACCESSION   DATMCV010000583 DATMCV010000000
VERSION     DATMCV010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433239"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VNU03_13475"
     CDS             complement(<1..542)
                     /locus_tag="VNU03_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWP75527.1"
                     /translation="MSDELFDQLNQLKQNESRVAVATLVNTRGTTPRKEGAKMVVGEG
                     GRVLGSVTIGGCVDAQVIEESADVMDKNAPRLLELNLGDEEAWEIGLTCGGTIEVFVE
                     PVTLGRTDDATLAFYEGARAHAETGGRAAIVTRLDPPHNGAKLLILDRGDREGSLGDP
                     FLDERFAAEARQALEAGKSRT"
     gene            complement(551..1705)
                     /locus_tag="VNU03_13480"
     CDS             complement(551..1705)
                     /locus_tag="VNU03_13480"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWP75528.1"
                     /translation="MARSRDLTTAVLRFGHMLRAAGMPLTIGEMMDGVRALDAVDLLD
                     RQDVYLALRTTLVARYEEFPIFDRCFETFWRFQAEEGQELEGLTGPGTPAIPEEHAGD
                     SGAEAAQKKVSVALEGWEEQGEDDGEPLEVPGVSEKEILMDQDFSSFPVEDLDEVARL
                     TILIAKRLARRISRRKRPTRRGGVIDMRRSFRANLMKGEIIELRRRERRRRKVRLVLL
                     CDVSGSMDLYSRFLLQFLYALQNVFGRVETFTFSTRLTRVTEHLKGASYRTALARLTE
                     VRDFSGGTRIGESLQEFNARWGSLVDRHTIVLLLSDGWDTGEPDILTNEMLTIKRKAG
                     RLIWLNPLLGNPSYEPLTRGMAAALPLVDHFAAAHNLASLRDLAGHLNLR"
     gene            complement(1708..2565)
                     /locus_tag="VNU03_13485"
     CDS             complement(1708..2565)
                     /locus_tag="VNU03_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWP75529.1"
                     /translation="MEGADYVTDPAIATSVHLAMTLKKPLLIEGHAGVGKTEIAKVMS
                     RMLHANLIRLQCYEGLDAAQALYEWNYPKQLLHIKLEESTTHSLREKEATIFSEPFLI
                     RRPLLQAISQDGKPPVLLIDEVDRADEEFEAFLLEVLSEFQVTIPEIGTLKATHPPYV
                     ILTSNRVRELSDALRRRCLYLWVDFPGFDKEVRIVMRKVPGVNEALARQISRFMESLR
                     SIRLAKVPGVAETLDWAQALASLHADHIDEELVIETLGCVLKDADDVKRFKRELTSSG
                     LKRFVAAEG"
BASE COUNT          374 a          901 c          859 g          432 t
ORIGIN      
        1 gtgcgcgact tccccgcctc cagcgcctgc cgggcttccg ccgcgaagcg ctcgtcgagg
       61 aatgggtccc ccaggctgcc ctcgcggtcg ccgcgatcga ggatcaggag cttggccccg
      121 ttgtggggcg ggtcgagccg cgtgacgatc gcggcgcggc ccccggtctc ggcgtgggcg
      181 cgcgcaccct cgtagaaggc gagcgtcgcg tcgtcggtcc ggccgagcgt caccggctcc
      241 acgaagacct cgatggtccc cccgcaggtg agcccgatct cccacgcctc ctcgtcgccc
      301 aggttcagct cgagcagccg cggggcgttc ttgtccatca cgtcggcgga ctcctcgatg
      361 acctgggcgt ccacgcaccc gccgattgtc accgagccga ggacgcggcc cccctcgccc
      421 accaccatct tggccccttc cttccggggc gtggtgccgc gggtgttgac gagcgtggcc
      481 accgccaccc tcgactcgtt ctgcttgagc tggttcagct gatcgaagag ctcgtcgctc
      541 atggtctcgc ttacctcagg ttcagatgcc cggcgaggtc ccggaggctc gccagattgt
      601 gcgccgccgc gaagtggtcc acgagcggca gcgccgcggc catgccgcgc gtcagcggct
      661 cgtaggatgg attgccgagc agcggattga gccagatgag ccgccccgcc ttgcgcttga
      721 tggtgagcat ctcgttggtg aggatgtccg gctcaccggt gtcccagccg tccgacagca
      781 ggagcacgat cgtgtgccga tccaccaggc tcccccaccg cgcgttgaac tcctgcagcg
      841 attcgccgat gcgcgtgccg ccggagaagt cccggacctc ggtcagccgg gccagcgccg
      901 tgcggtagga cgcacccttg agatgctcgg tgacccgggt cagtcgcgtg gagaacgtga
      961 aggtctccac ccggccgaag acgttctgga gcgcgtagag gaactggagg aggaaccggc
     1021 tgtagaggtc catggaaccc gagacgtcgc agagcaggac cagccggacc ttgcgcctcc
     1081 ggcgctcgcg tctccgcagc tcgatgatct ctcccttcat caggttggcc cggaagctgc
     1141 ggcgcatgtc gatgaccccg ccgcgccggg tcggccgctt gcgacggctg atccgccgcg
     1201 cgagccgctt ggcgatcagg atggtcaggc gcgccacctc gtccagatcc tcaaccggga
     1261 aggacgaaaa gtcctgatcc atgaggattt ccttctcgct aactccaggg acttcgaggg
     1321 gctctccgtc gtcctccccc tgctcctccc agccctccag cgcgaccgag accttcttct
     1381 gggccgcctc ggctccgctg tcccccgcgt gctcctcggg aatggcgggc gtgccgggcc
     1441 cggtgagccc ttcgagctcc tggccctcct cggcctggaa ccgccagaag gtctcgaagc
     1501 accggtcgaa gatcgggaac tcctcgtacc gggccaccag cgtggtgcgg agggccaggt
     1561 agacgtcctg ccggtcgagc agatccaccg cgtcgagggc ccgcacccca tccatcatct
     1621 cgccgatggt gagcggcatc ccggccgcgc gcagcatgtg gccgaagcgc agcaccgcgg
     1681 tggtgaggtc gcgagagcgg gccaccgcta cccctcggcg gccacgaagc gcttgagccc
     1741 gctcgaggtc agctcgcgct tgaagcgctt gacgtcgtcg gcgtccttga gcacgcaccc
     1801 gagggtctcg atgaccagct cctcgtcgat gtggtcggcg tggagcgagg ccagcgcctg
     1861 ggcccagtcc agggtctcgg ccacccccgg caccttggcg aggcggatgc tgcgcaggct
     1921 ctccatgaag cgcgagatct gccgggccag ggcctcgttg acgcccggca ccttgcgcat
     1981 cacgatccgg acctccttgt cgaagccggg gaagtccacc cagaggtaga gacagcggcg
     2041 ccggagcgcg tcggacagct cgcgcacccg gttcgacgtc aggatcacgt acggcggatg
     2101 cgtcgccttg agggtcccga tctcggggat cgtcacctgg aactccgaga ggacttccag
     2161 caagaacgct tcgaattcct cgtccgcccg atcgacctcg tcgatcagca gcaccggcgg
     2221 cttgccgtcc tggctgatcg cctgcagcag cggccggcgg atcaggaagg gctcggagaa
     2281 gatcgtggct tccttctccc gcaggctgtg ggtggtggac tcctcgagct tgatgtgcag
     2341 cagctgcttg gggtagttcc actcgtagag ggcctgcgcc gcgtcgagcc cctcgtagca
     2401 ctgcagccgg atcaggttgg cgtggagcat ccgcgacatc accttggcga tctcggtctt
     2461 gcccacgccc gcgtgcccct cgatgaggag aggcttcttg agggtcatgg ccaggtgcac
     2521 cgacgtggcg atggcgggat cggtgacgta gtcggcgcct tccatc
//
LOCUS       DATMCV010000584         5306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_298014, whole genome shotgun sequence.
ACCESSION   DATMCV010000584 DATMCV010000000
VERSION     DATMCV010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298014"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /gene="alaS"
                     /locus_tag="VNU03_13490"
     CDS             <1..1838
                     /gene="alaS"
                     /locus_tag="VNU03_13490"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWP75530.1"
                     /translation="ADHARAATFLIADGVTPSNEWRGYVLRRIMRRAMRHGRMLGLEA
                     PFLWEVTGSVVALMGDAYPEIREAQARVAETVRQEEERFAETLDLGMARIKEEIDRRA
                     PGSPAPMVDGRFLFTLYDTYGFPPDLAKEVFEDAGWRITAETDRIFDAEMEAQRERAR
                     AKASFGAGDAGGDPDVAIYQRLSSELAKPQFLGHGALAAPARVLALVADGQRRREARQ
                     GETVEIILDRTPAYAESGGQQGDTGLMVGREGQGTIEDTFYRGSQLIVHRVAVTQGHL
                     REGEEVAVSVESRRRQGLRQHHTGTHLLHAALRKVLGTHVGQAGSLVAPDHLRFDFSH
                     GASVKDGEIESVESLVNEQVQANVVVSPAEMDLQEALRTGAMALFGEKYGDRVRVIRI
                     GDFSTELCGGTHLDATGQIGLFKVTDEGAVASGVRRIEAVTGEHALAHVGREEAALRE
                     SAGLLKIPPLELPRRLAALLEDQKRLEKQLAQLESKLARTQAQDLVTSAREVAGVPVL
                     AARLDGLDPDGLRSVMDTLRDRLPSGVILLGSAGDGKVSLVAAVSKDLMKRFPAGRLV
                     QDVAKLVGGGGGGRPDLAQAGGKDASKLDEAIASVAGWVERMAKA"
     gene            1843..3876
                     /locus_tag="VNU03_13495"
     CDS             1843..3876
                     /locus_tag="VNU03_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP75531.1"
                     /translation="MATTGRRTVRTMCPMNCHPTLCGMLVTVEDGRLLEVAGDPDNPD
                     SRGFLCVRGRASREILDNPKRLLHPLVRDRRDAPLREAGWDEALERIADRMRAVGREA
                     VGLWSGHGFFATNYGVRVNSELLRRFANLWGCQWWAPTMICWGLGGFGIGLTGPLETN
                     TKEDLGAHAGLVVLWGANLASQPNTGRYLVAARRRGAHVVTIDVRRTEAAAQSDEVLI
                     LRPGTDAALALGIMHVLIAEGLHDREFVARHTVGFDALAAHVAPHTPEWTASVTGVDA
                     AAIVALARRYAASRPAMIVLGGSSMHKGANGWQGARAVACLPALTGNLGIAGGGLGPR
                     HGSKGHGHGLADIAEPGRRPPGHYVPNQMPRITEALEDGRVRALLLFGTDMLSSFADS
                     GRVERALTRQDLIVVQDLFLNDTARRFADVVLPGTAWVEELGCKATNTHLYLMEPALA
                     PAGQTRPGAWVLRELARRLGVADFYPWAGDEGPIDAILDHPATGHATVAALRAEGGMR
                     ALRVSHVGHPDLVFPTPSGKVEFYSSRARDLGLPPLPVHEPLPASSYPLVLRQGRTLT
                     HFHGFYDHGRALPSLAALEPEPVLWISPADATARGVGEGARIRVFNQRGEMAARARVT
                     DRVPAHTVWMRDGWDGLNRLTSGEACLPDDAVDVFGFSAGQAAFDAAVEVAPA"
     gene            3890..4552
                     /locus_tag="VNU03_13500"
     CDS             3890..4552
                     /locus_tag="VNU03_13500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP75532.1"
                     /translation="MLLDFGGTLDADGLTWKERFFRLWDAEGAAGSREAFDPVFYAAD
                     DALVGAIPRTLSFEDTVRRLTAGLADALAVRGAPVIERVAQRFLADAHRHLRRNHAIL
                     ERLRPRYRLGIVSNFYGNLDTVCNNVAILPFFGVIVDSEQVGCSKPDPRIFWTALEQL
                     GVTPADATFVGDSAARDMAGARALGMRHIWLTGEPAPPGGPCCPGDAMIHSLADVEAL
                     LF"
     gene            4552..5256
                     /locus_tag="VNU03_13505"
     CDS             4552..5256
                     /locus_tag="VNU03_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWP75533.1"
                     /translation="MAAPIRGGIIAAGAGSRLRADGWRVPKPLVEVAGLPLIGAVLRN
                     FNAAGITAITIIVNQEERDCVQWVRARYPDLEVDFIVKTTASSLESFQEVTARGPDGP
                     MLVSTVDAWCAAEDFARFAARAAARPDEATVLAVTPLVDDEKPLWVTLGAGGRVDAIG
                     VPGGGLVTAGVYRVSARARRVAAAPRLGRLREYLEWLHRSGEPLYGEIIETVVDVDRG
                     LDVAAAEALARGQRRA"
BASE COUNT          685 a         1898 c         1998 g          725 t
ORIGIN      
        1 tcgcggacca cgcgcgcgcg gccaccttcc tgatcgcgga cggggtgacc ccgtccaacg
       61 agtggcgcgg ctacgtgctg cggcggatca tgcggcgggc catgcgccac gggcggatgc
      121 tgggcctcga ggcgccgttc ctctgggagg tgaccggcag cgtggtggcg ctgatgggcg
      181 acgcgtatcc ggagatccgc gaggcgcagg cgcgggtggc cgagacggtg cgccaggaag
      241 aggagcgctt cgcggagacc ctcgatctcg gcatggcccg catcaaggag gagatcgatc
      301 gtcgcgcccc cggctctccc gcgccgatgg tcgatggacg cttcctgttc acgctctacg
      361 acacgtacgg gttcccgccc gatctggcga aggaagtgtt cgaggacgcg ggctggcgga
      421 tcaccgcgga aacggaccgg atcttcgacg ccgagatgga ggcgcagcgc gagcgcgccc
      481 gcgccaaggc gtcgttcggg gccggtgacg ccggcggcga tcccgacgtc gcgatctacc
      541 agcggctctc gagcgagctc gccaagccac agttcctcgg ccacggcgcg ctcgccgcgc
      601 ccgcgcgcgt cctggccctg gtggccgacg ggcagcggcg gcgggaagca cggcagggcg
      661 agaccgtgga gatcatcctc gaccgcaccc ccgcgtatgc ggagtcgggc ggccagcagg
      721 gcgacaccgg cctcatggtg ggacgcgagg ggcagggcac gatcgaggac acgttctacc
      781 gcggcagcca gctcatcgtg caccgggtgg cggtcaccca gggccacctg cgcgagggcg
      841 aggaggtggc ggtgagcgtg gagtcgcgcc ggcggcaggg actgcgccag caccacaccg
      901 ggacccacct gctccacgcg gccctacgaa aagtcctcgg cacgcacgtc ggccaggccg
      961 gctcgctggt ggcgccggac cacctgcgct tcgacttctc ccacggcgcg tcggtcaagg
     1021 atggcgagat cgagagcgtc gagagcctgg tcaacgagca ggtgcaggcc aacgtggtgg
     1081 tgagcccggc cgagatggac ctgcaggagg cgctgcgcac cggcgcgatg gccctcttcg
     1141 gcgagaagta cggcgaccgg gtgcgcgtca tccgcatcgg cgacttctcg accgagctct
     1201 gcggcggcac ccacctcgac gccaccgggc agatcggtct cttcaaggtg accgacgagg
     1261 gcgcggtggc ctccggggtg cgccgcatcg aggcggtcac cggcgagcac gcgctcgccc
     1321 acgtggggcg cgaggaggcg gcgctgcgcg agtcggccgg cctgctcaag atcccgccgc
     1381 tcgagcttcc ccgccggctc gccgcgctcc tggaggacca gaagcggctc gagaagcagc
     1441 tcgcccagct cgagtccaag ctcgcgcgca cccaggccca ggatctcgtc acctccgcgc
     1501 gagaggtggc gggggtgccg gtgctggccg cgcgcctcga cggcctcgac ccggacgggc
     1561 tccgctcggt gatggacacc ctgcgcgatc gcctgccgtc gggggtcatc ctcctcggca
     1621 gcgcgggcga cggcaaggtg agcctcgtgg ccgcggtgtc gaaggatctg atgaagcgct
     1681 tcccggccgg tcgtctcgtg caggacgtcg ccaagctggt gggcggcggc ggcggcggtc
     1741 ggcccgacct ggcccaggcc ggcggcaagg acgcatccaa gctcgacgag gccatcgcct
     1801 cggtcgccgg ctgggtggag cgcatggcca aggcgtagct tcatggcaac aacgggccgc
     1861 cgcaccgtgc gcaccatgtg cccgatgaat tgccatccca ccctgtgtgg catgctcgtc
     1921 accgtcgagg acgggcggct gctcgaggtc gcgggcgatc cggacaatcc cgacagccgc
     1981 ggcttcctgt gcgtgcgcgg gcgggcctcg cgcgagatcc tggacaatcc gaagcggctg
     2041 ctccatcccc tcgtgcgcga tcgccgggac gcgccgctgc gggaggcggg ctgggacgag
     2101 gcgctcgagc gcatcgccga ccgcatgcgc gcggtggggc gcgaggcggt cggtctctgg
     2161 tcgggccacg gcttcttcgc gaccaactac ggggtgcggg tgaactccga gctgctgcgc
     2221 cgcttcgcca acctctgggg ctgccagtgg tgggcgccca ccatgatctg ctgggggctc
     2281 ggcggcttcg ggatcgggct gaccggtccg ctcgagacca acaccaagga ggacctgggc
     2341 gcgcacgcgg gcctcgtcgt cctgtggggc gccaacctcg cgagccagcc caacaccggc
     2401 cggtacctgg tcgcggcccg tcgccgcggc gcccacgtcg tcaccatcga cgtgcgccgc
     2461 accgaggcgg ccgcccagtc cgacgaggtt ctgatcctgc ggcccggcac cgacgcggcg
     2521 ctcgcgctcg ggatcatgca cgtgctgatc gccgagggcc tgcacgatcg cgagttcgtc
     2581 gcgcgccaca ccgtcggctt cgacgccctc gcggcccacg tggcgccgca cactccggag
     2641 tggaccgcgt cggtgaccgg ggtcgacgcg gccgcgatcg tggctctcgc gcgccgctac
     2701 gccgcgagcc gccccgccat gatcgtgctc ggcggctcca gcatgcacaa gggcgccaac
     2761 ggctggcagg gcgcgcgggc ggtggcgtgt ctgcccgccc tcaccggcaa cctgggcatc
     2821 gcgggcggcg gcctcgggcc gcggcacggc agcaaggggc acggccacgg tctggccgac
     2881 atcgcggagc cggggcggcg cccgcccggc cactacgtgc cgaaccagat gccgcggatc
     2941 accgaggccc tcgaggacgg gcgcgtgcgc gcgctgctcc tcttcggcac cgacatgctc
     3001 tcctcgttcg cagacagcgg gcgggtcgag cgcgcgctca cccggcagga cctgatcgtc
     3061 gtccaggatc tcttcctcaa cgacaccgcg cggcgcttcg cggacgtggt cctgcccggc
     3121 accgcgtggg tcgaggagct gggctgcaag gccaccaaca cccatctcta cctgatggag
     3181 cccgcgctcg cccccgcggg ccagacgcgc cccggggcgt gggtgctgcg cgagctcgcg
     3241 cggcggctcg gggtcgccga cttctacccg tgggcgggcg acgagggacc catcgacgcc
     3301 atcctcgacc atcccgccac cggccacgcc acggtggccg cgctgcgcgc ggagggcggc
     3361 atgcgggcct tgcgggtctc gcacgtgggc cacccggacc tcgtctttcc caccccgtcc
     3421 ggcaaggtcg agttctactc gtcgcgcgcg cgcgacctcg ggctgccccc gctgccggtc
     3481 cacgagccgc tgcccgcgtc gtcctatccg ctggtgctgc gccaggggcg gacgctcacg
     3541 cacttccacg gcttctacga ccacggccgc gcgctgccct cgctggccgc gctcgagccg
     3601 gagccggtgc tctggatctc gcccgccgac gcgaccgccc gcggggtggg ggagggcgcg
     3661 cggatccgcg tgttcaacca gcgcggggag atggccgcgc gggcgcgcgt caccgaccgc
     3721 gtgcccgcgc acacggtgtg gatgcgcgac ggctgggacg ggctcaaccg gctcacctcg
     3781 ggcgaggcgt gcctgcccga cgacgcggtg gacgtcttcg ggttctcggc cggccaggcc
     3841 gcgttcgacg cggcggtgga ggtggcgcct gcctgatcgg ccgcgcgcgg tgctgctcga
     3901 cttcggcggc accctcgacg ccgacgggct cacctggaag gagcgcttct tccgcctctg
     3961 ggacgcggag ggtgcggccg gctcccgcga ggcgttcgat ccggtcttct acgcggcaga
     4021 cgacgccctc gtcggcgcga tcccgcggac gctctcgttc gaggacacgg tgcgccgcct
     4081 gaccgccggc ctggccgacg cgctcgcggt gcggggggcg ccggtgatcg agcgcgtggc
     4141 ccagcgcttc ctcgccgatg cgcatcgtca tctgcggcgc aaccacgcga ttctcgagcg
     4201 tctccggccc cgctatcgcc tcggcatcgt gtccaacttc tatggcaacc tcgacaccgt
     4261 gtgcaataat gtagcgattc tgccgttctt cggcgtcatc gtcgactccg agcaggtggg
     4321 atgctcgaag cccgatccgc gcatcttctg gacggcgctg gagcagctcg gggtgacccc
     4381 cgcggacgcc accttcgtgg gcgattcggc ggcccgggac atggcgggcg cgcgggcgct
     4441 cggcatgcgg cacatctggc tgaccggaga gcccgcgccg cccggcgggc cgtgttgccc
     4501 tggtgacgcg atgatccact cgctggcgga cgtggaggca ctgctgttct gatggcggca
     4561 ccgatccggg ggggcatcat cgcggcgggg gcgggcagtc gcctgcgcgc ggacggctgg
     4621 cgcgtcccca agccgctggt cgaggtggcg ggtctgccgc tcatcggcgc ggtcctgcgc
     4681 aacttcaacg cggccgggat caccgcgatc acgatcatcg tgaaccagga ggagcgggac
     4741 tgcgtccaat gggtgcgggc gcgatacccg gacctcgagg tcgacttcat cgtgaagacc
     4801 accgcctcgt cgctggagag ctttcaggag gtgaccgccc ggggcccgga cggtcccatg
     4861 ctcgtttcca cggtggacgc gtggtgcgcg gcggaggact tcgcgcggtt cgccgctcgg
     4921 gccgccgcgc ggccggacga ggcgaccgtg ctggcggtga ccccgctcgt cgacgacgag
     4981 aagccgctct gggtgaccct cggcgcgggc gggcgagtgg acgcgatcgg cgtgcctggc
     5041 ggtggcctgg tcaccgccgg ggtgtaccgc gtctccgcgc gggcgcggcg ggtcgcggcc
     5101 gcgccgcggc tcgggcgcct gcgcgagtac ctcgagtggc tgcaccggag cggcgagccg
     5161 ctctacggcg agatcatcga gacggtggtg gacgtggacc gcggcctgga cgtggcggcg
     5221 gcggaggcgc tcgcgcgagg acaacggcgc gcatgagcct gacgcgcatg agcctgagag
     5281 gaggagcggg gtgagcgagc ggacct
//
LOCUS       DATMCV010000585         5288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_828207, whole genome shotgun sequence.
ACCESSION   DATMCV010000585 DATMCV010000000
VERSION     DATMCV010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828207"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VNU03_13510"
     CDS             complement(<1..646)
                     /locus_tag="VNU03_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HWP75534.1"
                     /translation="MTAVRHLLDAIKFEHTVFALPFAYVAMVLAADGWPGWRTLLWVT
                     LAMAGARTLAMAVNRLADRFIDAANPRTARRHLPAGLLSPAQVAGAAAAAGALLLLSA
                     YMLNPLCLALAPLAVLFLVGYSYTKRFTWLSHWILGFTDGIAAAGGWIAVRGAFAPPV
                     YVLWFALTVWIAGFDLIYACQDVEFDREHRLHSVPARFGIPAALATARVCHVLTI"
     gene            complement(660..2111)
                     /locus_tag="VNU03_13515"
     CDS             complement(660..2111)
                     /locus_tag="VNU03_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HWP75535.1"
                     /translation="MAFGDLREFIGHLEKHGWLRRVSASVSRDLEIAEITDRVSKSPG
                     GRNVALLFENVRGSEMPVLINAFGSPERMAAALGVERLDEISARVAKLLDLRMPGSMF
                     DKLRKLGDLFDLAKAGPKRVRSAACQEVVETDRPSLAALPILRCWPGDGGRYITLPMV
                     FTRDPVTGARNVGMYRLQVYDDQTLGMHWQIHKGSAEHQRLAEERREPMEVAIALGGD
                     PCAIYSGSAPLPPGVDEMVFAGWLRGSGVPMVPCKTVHIDVPAEAEIVLEGWVDPAER
                     RLEGPFGDHTGYYSLARDYPVFHLKAITRRRSPIYPTTIVGRPPQEDYWLGKATERIF
                     LPIIRMMLPEVVDMNMPAEGVFHNLVIVSIRKRYPGHARKVMYALWGLGLMMLAKNIV
                     VVSDHVNVHDLSEVAWRATGNIDPRRDLVIVDGPMDDLDHAAMRHRFGGKLGVDATEK
                     MEADGIGQPWPEEIVMSEDVRALVTRRWAEYGL"
     gene            complement(2128..2637)
                     /locus_tag="VNU03_13520"
     CDS             complement(2128..2637)
                     /locus_tag="VNU03_13520"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWP75536.1"
                     /translation="MPRAGFFDVSFLVLSSLAIVFAFVAYAKDPSLPAVGARNGLALL
                     WFIIPRLVPALILAGMIQVVIPQETVGRYFGQQSGLSAIFIASAAGVITPGGPMVSVP
                     LLVVLSNSGMALGPLVAYMTAWSLFGVQRIIAWEAPLMGWRFVAVRTVSSLVFPIVAG
                     WLVKIYFHE"
     gene            2684..4168
                     /locus_tag="VNU03_13525"
     CDS             2684..4168
                     /locus_tag="VNU03_13525"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP75537.1"
                     /translation="MRWTIRRLVLALALPLAVGVAFKMWLGTGPALVAAALGAVLVGW
                     TAIGYAVREAAAQQRAAASGLQWLQALAQVSLSLSQGLDRNRLLRQITDALATLTSAH
                     NVVLWEVDTASSLLVRRAWTTDPSIGAMELPSSLTLQQGGTGWIARHREALFVDDIAR
                     DARIMAADWALSRDLVAFAGAPVAAGDELLGVLTLNLKRGRLPEGNDRALLLSFASQA
                     AVAMRNARLFADNTRLYEEAQQRVRELQDTQAQLVQVAKLSAVGQLVSGVAHELNNPL
                     SVVIGYGQLLLARGVPEEARRPIELMIAQGARMAKIVQSLMLFSRQRKPERRAVSLPD
                     IIQQIITLRETQLAISSIRLETEFGADVPPVQGDSHQLQQVLLNLILNAEQAILGSGV
                     GGRRSGDCIRVSTSARLEGDRAWVVAQVVDNGPGIPAEVLPRIFEPFFTTKKVGEGAG
                     LGLSVSYGIVEEHGGRLSVESGPGRSVFTLELPAAAPVSAGVSA"
     gene            4203..4475
                     /locus_tag="VNU03_13530"
     CDS             4203..4475
                     /locus_tag="VNU03_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWP75538.1"
                     /translation="MVKAELVAAMAKASGGSKVSAEKALNALLDNVFDSLRRGRKVTI
                     GGFGTFLVSRRAERNGRNPRTGKAIKIPACRVPRFKPSRSLKSHLK"
     gene            4551..4624
                     /locus_tag="VNU03_13535"
     tRNA            4551..4624
                     /locus_tag="VNU03_13535"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4583..4585,aa:Gln,seq:ctg)
     gene            4684..4759
                     /locus_tag="VNU03_13540"
     tRNA            4684..4759
                     /locus_tag="VNU03_13540"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4718..4720,aa:Glu,seq:ctc)
     gene            complement(4838..>5288)
                     /locus_tag="VNU03_13545"
     CDS             complement(4838..>5288)
                     /locus_tag="VNU03_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWP75539.1"
                     /translation="DWGFRNVRVKGGKTRDIPLPRAVTQFLQTYVAQHLPTLTDHITA
                     DTPLFWSSWGQRRQGKIRRPITGKNIWRLCKTYGRIIGYSMLKPHDLRHGVAMEILEH
                     HHDLEAVRAMPGHTRIDTTQTYARIRPAELKRTMEFYEDRAVGLLDT"
BASE COUNT          815 a         1801 c         1888 g          784 t
ORIGIN      
        1 cgatggtcag cacgtggcac actcgcgcgg tggcgagcgc ggcggggatg ccgaagcgcg
       61 cgggaaccga gtgcagccgg tgctcacggt cgaactcgac gtcctggcag gcgtagatga
      121 gatcgaagcc ggcgatccac accgtgaggg cgaaccacag cacgtagacc ggcggcgcga
      181 aggcgccgcg caccgcgatc cagccgcccg ccgccgcgat gccgtcggtg aagcccagga
      241 tccagtgcga gagccaggtg aatcgcttgg tgtaggagta gcccaccagg aacaggaccg
      301 cgagcggggc gagggcgagg cagagcgggt tcagcatgta ggccgagagc agcaggagcg
      361 cgcccgccgc cgcggccgcg ccggcgacct gggcggggct gagcaggccc gcgggcaggt
      421 gccgccgggc ggtgcggggg ttggccgcgt cgatgaagcg atcggccagg cggttgaccg
      481 ccatggccag ggtgcgggcg cccgccatcg ccagggtcac ccagagcagg gtgcgccagc
      541 ccggccagcc gtcggcggcg agcaccatcg ccacgtaggc gaagggcagg gcgaagacgg
      601 tgtgctcgaa cttgatcgcg tcgagcaggt ggcggacggc ggtcacggag cggcccgcgc
      661 tagaggccgt actcggccca gcgccgggtg accagcgccc ggacgtcctc gctcatcacg
      721 atctcctccg gccacggctg cccgatgccg tcggcctcca tcttttcggt ggcgtccacc
      781 ccgagcttgc cgccgaagcg gtggcgcatg gccgcgtggt ccaggtcgtc catgggcccg
      841 tccacgatga cgaggtcgcg ccggggatcg atgttgccgg tggcccgcca cgccacctcc
      901 gagaggtcgt gcacgttgac gtgatcggag accaccacga tgttcttggc cagcatcatg
      961 agcccgaggc cccagagcgc gtacatgacc ttgcgcgcat ggcccgggta gcgcttccgg
     1021 atcgagacga tcaccaggtt gtggaagacg ccctcggcgg gcatgttcat gtccaccacc
     1081 tcgggcagca tcatccggat gatcggcagg aagatgcgct cggtggcctt gcccagccag
     1141 tagtcctcct gcggcggccg ccccacgatg gtggtcgggt agatcgggct cctccggcgc
     1201 gtgatcgcct tgaggtggaa gaccgggtag tcgcgcgcga gggagtagta gccggtgtgg
     1261 tcgccgaacg ggccctcgag ccgccgctcg gccggatcga cccagccctc cagcacgatc
     1321 tcggcctcgg cgggcacgtc gatgtggacg gtcttgcatg gcaccatcgg cacgcccgac
     1381 ccgcgcagcc agcccgcgaa gaccatctcg tcgacgccgg gcggcaaggg cgcggagccc
     1441 gagtagatcg cgcacggatc accgcccagc gcgatggcca cctccatcgg ctcgcgccgc
     1501 tcctcggcca gccgctggtg ctcggcggag cccttgtgga tctgccagtg catgccgagg
     1561 gtctggtcgt cgtagacctg gagccggtac atccccacgt tgcgcgcgcc ggtgaccggg
     1621 tcccgggtga acaccatggg cagcgtgatg tagcggccgc cgtcgcccgg ccagcagcgc
     1681 aggatgggga gcgcggccag ggagggccgg tcggtctcga ccacctcctg gcaggcggcc
     1741 gagcgcaccc gcttggggcc ggccttggcg aggtcgaaga ggtcgcccag cttgcggagc
     1801 ttgtcgaaca tcgagcccgg catgcggagg tcgaggagct tggcgacccg ggcggagatc
     1861 tcgtcgagcc gctcgacgcc gagcgcggcg gccatccgct ccggcgagcc gaaggcgttg
     1921 atgaggaccg gcatctccga gccgcgcacg ttctcgaaga ggagggccac gttgcgcccg
     1981 cccggactct tggacacccg gtcggtgatc tcggcgatct cgaggtcgcg cgagaccgac
     2041 gcgctcacgc ggcggagcca gccgtgcttc tcgaggtggc cgatgaactc gcggagatcg
     2101 ccgaaggcca tggcggcgcg ctgagcgcta ctcgtggaag tagatcttga cgagccagcc
     2161 ggcgacgatg gggaagacca ggctcgacac ggtgcgcacc gccacgaagc gccagcccat
     2221 gagcggagcc tcccacgcga tgatccgctg caccccgaag agggaccacg cggtcatgta
     2281 ggcgaccagg ggaccgagcg ccatgcccga gttcgacagc accacgagca ggggcacgct
     2341 caccatcggc ccgcccgggg tgatcacgcc tgccgcgctc gcgatgaaga tggcgctcaa
     2401 ccccgactgc tgcccgaagt agcgcccgac cgtctcctgc gggatcacca cctggatcat
     2461 gccggcgagg atcagggccg gcaccaggcg cgggatgatg aaccagagca gggcgaggcc
     2521 gttccgggcg cccaccgccg ggaggctcgg atccttcgcg taggccacga acgcgaagac
     2581 gatggccagc gaggagagaa ccaggaagga aacgtcgaag aatccggccc gcggcatggc
     2641 ggcattatac gtcacgtggg ctatactgac ctcgctccga gcgatgcgct ggacgattcg
     2701 acggctcgtg ctcgccctgg cactgcctct ggcggtgggt gtcgccttca agatgtggct
     2761 cggcaccggc cccgcgctcg tcgcggcagc tctcggcgcg gtgctggtcg gctggaccgc
     2821 gatcggctac gcggtccggg aggccgccgc gcagcagcgg gcggccgcct ccgggctcca
     2881 gtggctccag gctctcgccc aggtgagcct ctcgctctcg cagggcctcg accgcaatcg
     2941 gctcctccgc cagatcaccg acgcgctcgc caccctgacc tccgcccaca acgtcgtgct
     3001 gtgggaggtg gacaccgcct cgagcctgct ggtgcggcgc gcctggacca cggatccgtc
     3061 gatcggggcc atggagctgc cgtcctcgct gaccctgcag cagggcggca ccggctggat
     3121 cgcccgccac cgcgaggcgc tcttcgtcga cgacatcgcg cgggacgcgc ggatcatggc
     3181 cgccgactgg gccctcagcc gggacctggt cgccttcgcg ggggccccgg tggcggcggg
     3241 cgacgagctg ctgggtgtgc tcacgctcaa cctcaagcgc ggccgcctgc ccgagggcaa
     3301 cgatcgcgcg ctgctcctgt ccttcgcctc gcaggccgcg gtcgccatgc gaaacgcgcg
     3361 gctctttgcc gacaacacgc ggctctacga ggaggcgcag cagcgggtgc gcgagctgca
     3421 ggacacgcag gcccagctgg tgcaggtcgc caagctgtcc gcggtgggtc agctcgtctc
     3481 cggggtggcc cacgagctga acaacccgct gtcggtggtg atcggctacg gccagctcct
     3541 gctcgcccgg ggcgtgccgg aggaggcgcg ccggccgatc gagctgatga tcgcgcaggg
     3601 cgcgcgcatg gcgaagatcg tccagagcct catgctcttc tcccgccagc gcaagccgga
     3661 gcgtcgcgcg gtgagcctgc ccgacatcat ccagcagatc atcaccctgc gtgagacgca
     3721 gctcgcgatc tcgagcatcc ggctcgagac cgagttcggc gccgacgtgc ccccggtgca
     3781 gggtgattcc caccagctcc agcaggtgct cctgaacctg atcctgaacg ccgagcaggc
     3841 gatcctgggc agcggggtgg ggggccggcg ctccggcgac tgcatccgcg tcagcacgag
     3901 cgcgcggctc gaaggggacc gcgcctgggt cgtcgcgcag gtggtcgaca atggccccgg
     3961 catcccggcc gaggtgctcc cccgcatctt cgagccgttc ttcacgacca agaaggtggg
     4021 ggagggcgcg gggctcggcc tgagcgtctc gtacggcatc gtcgaggagc acggcgggcg
     4081 cctctcggtg gagagcggcc cggggcggag cgtgttcacg ctcgagctgc cggccgccgc
     4141 accggtgagc gccggcgtga gcgcttgact tcggcccgag cgctcgcgta gtaaggggtc
     4201 ccatggtcaa ggccgagctc gtcgccgcga tggcgaaggc ctccggcggc tcgaaggtct
     4261 ccgccgagaa ggcgctcaat gccctgctcg acaacgtgtt cgattcgctc cgtcgcggcc
     4321 ggaaggtgac catcggcggc ttcggcacct tcctggtgag ccggcgggcg gagcgcaacg
     4381 gccgcaatcc gcggaccggc aaggccatca agatccccgc gtgccgggtg ccgcgcttca
     4441 agccgagccg ctcgctcaag tctcacctga agtgacgcac cgtccgctaa ctccgcgtcc
     4501 gtcctgcctt gacaccccgc ggcgccgccc cgtacaatga ccgtcgccgc tgggggttcg
     4561 tctaatggta ggacgccagg ttctggccct ggtaatgggg gttcgagccc tccaccccca
     4621 gccatttaag cttcgccgtg tcggtcaggt gggtctacgg cgcgtgtagc acggtaggga
     4681 ggcggcccca tcgtctagag gcctaggaca tggccctctc aaggctaaaa cacgggttcg
     4741 aatcccgttg gggccaccaa attgtttttg ttgatgtttt atcctccggg tttgaggaac
     4801 tttcaaaagt tcttcaaacc cggaatcgtt tttctcctca cgtgtccagc aaacccacgg
     4861 ccctgtcctc gtagaactcc atcgtccgct tgagctcggc cgggcggatc cgcgcgtacg
     4921 tctgcgtggt gtcgatgcgc gtgtgcccgg gcatcgcgcg caccgcttcg agatcgtgat
     4981 gatgctcgag gatctccatc gcgacgccgt gacggagatc gtggggcttc aacatcgagt
     5041 agccgatgat ccggccgtag gtcttgcaga gccgccagat gttcttgccg gtaatcggcc
     5101 ggcggatctt gccctggcgg cgctggcccc agctcgacca gaagagcggt gtatcggccg
     5161 tgatgtggtc ggtgagcgtg ggcaggtgct gcgcaacgta ggtctgcagg aactgggtca
     5221 ccgcccgggg caggggaatg tcgcgggtct tgccgccctt gacgcggacg ttccgaaacc
     5281 cccagtcg
//
LOCUS       DATMCV010000586         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_967674, whole genome shotgun sequence.
ACCESSION   DATMCV010000586 DATMCV010000000
VERSION     DATMCV010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967674"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VNU03_13550"
     CDS             <1..462
                     /locus_tag="VNU03_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75540.1"
                     /translation="QSIKLTGAGFLTEPDVLPAQGKSALGVITGHFYTPLLDNPTNQR
                     FVKEFQAKFGGKMPDGFACQGYDTAEVIVRALKAVNGNTQDKPKLVEVIEKSEFDSPR
                     GRFRFDPKTHNVIQPFIYIREVREVAGGINNVPIDKVANVTDPGTGCTLPA"
     gene            534..1400
                     /locus_tag="VNU03_13555"
     CDS             534..1400
                     /locus_tag="VNU03_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75541.1"
                     /translation="MPGDLFAQALNGLAYGVLLFLLSVGLTLIFGMLDVVNLAHGSFY
                     MLGAYAGLSLIAGTGSFWLALLVAPLAVGLIGAVIERSCLRPLYRRPPLDQVLLTFGF
                     IYLFEDLVKWLWGGRIRSIPPPDLFSGSVSVLGAIIPSYRLFVIVFGLAVAVALFFLI
                     ERTRLGAVIRAGVFDAEMTGGLGINIHRVFTAVFAFGAALGGLSGVIAGPIQSAYPSM
                     GVTILIPALIVVVVGGLGSLKGSLIGSLLIGQAETFGKAWLPEASMLIIYVVMAAIML
                     VRPQGLFGRPLK"
     gene            1397..>2141
                     /locus_tag="VNU03_13560"
     CDS             1397..>2141
                     /locus_tag="VNU03_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75542.1"
                     /translation="MTGLRLLASALVVVALALFPLGAGNYPVKLLQEILIWGIFAMSL
                     DLLMGYAGMVSFGHSAFFGIGGYVAALALARSPGLVSALLLPAVAAGLAALLIGFFSI
                     RVSGVYFIMLTLAFSQMFHAYTFQVSWLGAEDGLVGIPRPTVPGVDVASVRGFHAYLL
                     ALAVLVALALWRIVRSPFGAVLRGIHENEARMEALGYAVDRYKLLAFVIAGIVAGVAG
                     SLYTQFNGSITPDAFYWKTSGEALLMVIIG"
BASE COUNT          258 a          714 c          747 g          422 t
ORIGIN      
        1 cagtcgatca agctcaccgg ggccggcttc ctcaccgagc ccgacgtgct gcccgctcag
       61 ggcaagtcgg ccctcggggt gatcaccggc cacttctaca ccccgctgct cgacaatccg
      121 accaaccagc gcttcgtgaa ggagttccag gccaagttcg ggggcaagat gccggacggc
      181 ttcgcgtgcc agggctacga caccgcggag gtcatcgtgc gcgcgctcaa ggcggtgaac
      241 ggcaacaccc aggacaagcc gaagctggtc gaggtgatcg agaagagcga gttcgacagc
      301 ccgcgtgggc ggttccggtt cgatcccaag acgcacaacg tgatccagcc gttcatctac
      361 atccgcgagg tgcgcgaggt ggcggggggg atcaacaacg tgccgatcga caaggtcgcg
      421 aacgtgaccg acccgggtac gggctgcacg ctgcccgcgt agtgccgctc ccgttgtgga
      481 cgtgcgcaac cccttcccgg ctgatcgctt cgcgtcgatt caggtggttc tcgatgccgg
      541 gtgatctgtt tgcgcaggcg ttgaatgggc tcgcctacgg cgtgctgctg ttcttgctgt
      601 cggtggggct cacgttgatt tttgggatgc tggatgtggt gaatctggcg catgggtcgt
      661 tttacatgct gggggcgtat gcggggctgt cgctgatcgc ggggacgggg agcttctggc
      721 tcgcgctgct cgtcgcgccg ctcgcggtcg gcctgatcgg ggcggtgatc gagcggtcgt
      781 gcctgcggcc gctgtaccgg cggccgccgc tcgaccaggt gctgctgacc ttcgggttca
      841 tctatctgtt cgaggatctg gtgaagtggc tctggggcgg gcgcatccgc tcgatcccgc
      901 cgcccgatct gttctcgggc tcggtctcgg tcctaggggc gatcatcccg tcctaccggc
      961 tcttcgtgat cgtcttcggg ctcgcggtgg cggtcgcgct gttcttcctg atcgagcgta
     1021 cgcgcctggg cgcggtcatc cgcgccgggg tcttcgacgc ggagatgacc ggcggcctcg
     1081 gcatcaacat ccaccgggtg ttcaccgccg tcttcgcctt cggcgccgcc ctcggcgggc
     1141 tctccggggt gatcgcgggg cccatccagt cggcctaccc gtccatgggc gtgacgatcc
     1201 tgatccccgc gctgatcgtg gtggtggtgg gcggcctcgg cagcctgaag ggctcgctga
     1261 tcggcagcct cctgatcggc caggccgaga ccttcggcaa ggcgtggctg cccgaggcct
     1321 ccatgctgat catctacgtg gtgatggccg ccatcatgct ggtgcgaccg caggggctct
     1381 tcggccgccc gctcaagtga cgggcctccg gctcctcgcc tcggcgctgg tggtggtcgc
     1441 gctggccctg ttccccctcg gggccggcaa ctacccggtg aagctcctcc aggagatcct
     1501 catctggggc atcttcgcga tgagcctcga tctgctgatg ggctacgcgg ggatggtctc
     1561 gttcggccac tccgcgttct tcggcatcgg cggctacgtg gccgcgctcg cgctggcccg
     1621 gagcccgggg ctcgtgtcgg ccctgctcct gcccgcggtg gccgccggcc tcgccgcgct
     1681 cctgatcggc ttcttctcga tccgggtgag cggcgtctac ttcatcatgc tcacgctggc
     1741 gttctcgcag atgttccacg cctacacctt ccaggtctcc tggctcgggg ccgaggacgg
     1801 cctcgtcggc atcccgcgcc ccacggtgcc gggcgtggac gtcgcgagcg tgcgcggctt
     1861 ccacgcctac ctgctcgcgc tggcggtgct ggtggcgctc gcgctctggc gcatcgtccg
     1921 ctcgccgttc ggcgccgtgc tgcgcggcat ccacgagaac gaagcgcgca tggaggcgct
     1981 gggctacgcg gtggaccgct acaagctgct ggccttcgtg atcgcgggga tcgtcgcggg
     2041 ggtggccggc tcgctctaca cccagttcaa cgggtcgatc accccggacg cgttctactg
     2101 gaagacgtcg ggcgaggcgc tgctgatggt gatcatcggc g
//
LOCUS       DATMCV010000587         8122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_498205, whole genome shotgun sequence.
ACCESSION   DATMCV010000587 DATMCV010000000
VERSION     DATMCV010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498205"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..568)
                     /locus_tag="VNU03_13565"
     CDS             complement(110..568)
                     /locus_tag="VNU03_13565"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HWP75543.1"
                     /translation="MSAPTNAKVHLHMHVSDLAKSREFYQKFLGADPVKVKANYAKFL
                     PDWAPVNLALSGGGSASEGTVNHVGVQVDAVDTVMAHLARVKAAGLPVREEMGVDCCH
                     ANQDKFWVRDPDGVEWEVYHLNYDLEGDEDAPKIKSVTFPMTKGNTCCAS"
     gene            complement(582..890)
                     /locus_tag="VNU03_13570"
     CDS             complement(582..890)
                     /locus_tag="VNU03_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWP75544.1"
                     /translation="MKSIARVPIARLEAAPEHVEAFKALAHLTRLQVFFFLVRAGKEM
                     SVGEIQEEVEIPGPTLSHHLDQLRRAGLVESRKEERYIYYSVKRDQVTALVRLLTACC
                     "
     gene            993..2300
                     /locus_tag="VNU03_13575"
     CDS             993..2300
                     /locus_tag="VNU03_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75545.1"
                     /translation="MTFAERLVGGRVHYAWIIAGVTFLILLTAAAIRATPGVLIVPLE
                     QAFGWSRATISFAVSVNLLLYGLCGPFAASLMERFGVRRVTLLSLCAVTVGVALTTVM
                     RSPWQLVLLWGVVVGIGTGAMAMVLAATIANRWFVARRGLVIGILTASTATGQLLFLP
                     LLAAVVQRTGWRAAALVVTGVALLLVPIVVIFMRDQPRDLGLRAFGDTPEAVSPARPA
                     TRPAGAIAALVEGSRSRDFWLLAGTFFICGLSTNGLIGTHLIPASMDHGIPAVTAAGL
                     LAAMGVFDLIGTTVSGWLSDRWDNRWLLFGYYGLRGLSLMVLPYAYGSEYFGLTLFAV
                     FYGLDWVATVPPTVRLTETLFGKHRVGIMYGWIAVAHQMGAATAAFGAGLLRTWFGSY
                     QVAFTGAGFVCLIAALMVLAIGHGQIVTGLRRRRVEISGAVEG"
     gene            complement(2297..3448)
                     /gene="solA"
                     /locus_tag="VNU03_13580"
     CDS             complement(2297..3448)
                     /gene="solA"
                     /locus_tag="VNU03_13580"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HWP75546.1"
                     /translation="MAFDVIVIGVGGMGSATAYHLARRKKKVLGIEQFDIPHQLGSSH
                     GVNRIIRLAYAEHPSYVPLLRRAYELWRELENSTGERLLVVTGGLDVGPEDGTLVAGS
                     VRSCHEHGIPHELMSAAAVGRRFPGYRLARGMVSVYQADAGFVMAERSVVAHANGALA
                     HGAEIHGRERVLDWKVGRGGVRVRTDRGSYEAERLVLTAGAWSGKLAGALAPVAKPER
                     QVMLWTQPRRPERFRVGAFPVFNMESPEGRFYGFPIHGIPGFKIGKYHHREQQVDPDS
                     IDRDCDARDEAVVREGIRRYFPDADGPTLSMAACMFTNTPDEHFILDVHPDAPRVSIA
                     AGFSGHGFKFCSVVGEIMADLATAGRTRWNVELFRLARPALRAAASGRR"
     gene            complement(3490..4494)
                     /locus_tag="VNU03_13585"
     CDS             complement(3490..4494)
                     /locus_tag="VNU03_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWP75547.1"
                     /translation="MPTPRPDTTRLQRLAHSYRDSAALMAAVELGLFTRVARGAETEA
                     AVARALDLTPTNAERLVTACVALGLLVRDGDRVRNAPDVARFLVEGEPTYAGPWMLFS
                     KPDWNEWGRLAGHLGRRDSVVLGKYAHGFTVEQARRYHEATYSIGMGAARRFVRQVDL
                     AGRRQILDLGGGSGCYCIVAAQQHPELRAIVFDLPPVVEVTREFIASHGLADRVTAQA
                     GDFTRDPLPADADVVIMASNLPQYSREIIQAVITRAFAALTPGGEMHLIGEMLDDGRG
                     GPRDPALWGLAEALWGSTGVAHSVAECRAYFERADARDVAVHEFVAGVLTRVTGRRPG
                     "
     gene            4617..6194
                     /locus_tag="VNU03_13590"
     CDS             4617..6194
                     /locus_tag="VNU03_13590"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP75548.1"
                     /translation="MPGIDRRRFFNLVGRNGGAAALYSTLGAMGIVAPSTPYRALTRP
                     PKDSGRGIRIAVLGAGIAGMSAAYELQRAGYECTVLEARDRPGGRIWTIRSGDTIAET
                     DSSQRVAWDRQPDLYLNAGPARISQHHQAMLSYCRELRVPLEVLVNDNRAALLQDDAA
                     FGGEPQVARRVLADTRGGIAALAAGALTPGARQSRSDRNLRTLLQGFGALDDDLRYAG
                     SVRAGYAEPPGLTPGQPGRRHPALPLAEIARAASWPSAMAAAETWDQAATMLQPVGGM
                     DAIARAFARALGTTIRYQAEVVGIRRIGDRARVSWRDRKQGSPHALDADFVICTIPLP
                     VLSGIESDFPDPVKRAIEVGALAYIPAVKVAFQANRRWWETDQQIYGGISWTSRDITQ
                     MWYPSAGLNGDKGILLGAYIWTTSIGRRFAAMSPAQRLAAAIADGERLHPGYAGLVDK
                     GVSIAWSKIPFTGGGWVDWGNAAWRAAYPALLNGHGAVHFAGEHMSYMNSWQEGAVRS
                     AHDIVERIAARKRDRAR"
     gene            complement(6222..7328)
                     /locus_tag="VNU03_13595"
     CDS             complement(6222..7328)
                     /locus_tag="VNU03_13595"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWP75549.1"
                     /translation="MERPLRVGFIGCGEVGAEKHLPAVREVRELEVVAVADVDPERLR
                     SVEARYGIPRLHRGAASLLADREIDVVAVCLPPRLQVAAALAALEADKHVWIEAPIGL
                     SLPDCDRLIAKARASGRQVTVGFHMRWHRLVRQAKEIVDRGDLGPIQTIRATWNSPRA
                     DDTLPEWRRHRAQGGGALVEVASDHFDLWRYLLGAEIAELFATTVDGRWEDEAAVVSG
                     HMSNGVLVSAVFSERANHDLEFEICGKAGRLRVSLIRFEGLEYYPTETMPSSLPARLG
                     RMGHFLRELPRALPRMHRAGDYRVSYCDQWRQFARSIRTGTPMEATLHDGRQALVAVL
                     AAARSAAVRRPVAMREAPSGMTLPTGTGPAQAAS"
     gene            complement(7376..7858)
                     /locus_tag="VNU03_13600"
     CDS             complement(7376..7858)
                     /locus_tag="VNU03_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018303416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWP75550.1"
                     /translation="MVLRHVVSTLTLEQATTIATRAIAAGRREKMLPLAVVVVDGGGQ
                     LVTLQRDDGCGTLRVRIAQGKARGALGMGIPSRTIRDRLQNRPAFQSAVATASQGQFI
                     PVPGGVLILDRDGRAIGAVGISGDTSDRDEYCAIEGVKAAGLGCDPPEPAPEWRESGL
                     "
     gene            complement(7893..>8122)
                     /locus_tag="VNU03_13605"
     CDS             complement(7893..>8122)
                     /locus_tag="VNU03_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75551.1"
                     /translation="AVLFITHDVEEALLLADTVCVMTARPGRIKTRLTVDLPRPRTLE
                     ATTSPRFNELKREVLGLIREESRRAAREPAR"
BASE COUNT         1101 a         2958 c         2838 g         1225 t
ORIGIN      
        1 cacgagccgt cggcagtcgt cacgcgaagc gcggcccggg cgtacttgtc cctcacgatg
       61 tccttgatgt cctgatctgc catggttcct ctccctgggg tggccgcgct caggacgcgc
      121 agcaggtgtt gcccttggtc atcggaaagg tcacactctt aatcttgggc gcgtcctcgt
      181 ccccctcgag gtcgtagttc aggtggtaga cctcccactc caccccgtcc ggatcgcgga
      241 cccagaactt gtcctggttg gcgtggcagc agtcgacccc catctcctcg cgcaccggca
      301 gcccggccgc cttgacgcgg gcgaggtggg ccatgacggt gtccaccgcg tccacctgca
      361 cgccgacgtg attcacggtg ccctcgctcg cggacccgcc gccggacagc gcgaggttga
      421 ccggcgccca gtcgggaagg aacttcgcgt agttcgcctt caccttgacc gggtccgcgc
      481 cgaggaactt ctggtagaac tcgcggctct tggccaggtc ggacacgtgc atgtgcagat
      541 ggaccttcgc attggtgggt gcgctcatgg cgttctcctc gtcagcagca ggcggtgagc
      601 aggcgcacca gcgccgtcac ctgatcccgc ttcacggaat agtagatgta gcgctcttcc
      661 ttccggctct cgaccagccc ggcgcgtctc agctggtcga ggtggtgcga cagcgtcggg
      721 cccgggatct ccacctcctc ctggatctcc ccgaccgaca tctccttgcc cgcccgcacc
      781 aggaagaaga acacctgcag ccgcgtgagg tgggcgagag ccttgaaggc ctccacgtgc
      841 tcgggggcgg cctcgagccg ggcgatcggc acccgcgcga tgcttttcat agtttgaaat
      901 atatgaaact acagaaccga tgtcaacccc atccggaaca gggccgcggc ctccgctggc
      961 ccaatgcata tacatgtatt accctgggcc cgatgacctt cgcggagcgg ctggtcggcg
     1021 gccgggtgca ctacgcctgg atcatcgccg gcgtcacctt cctgatcctc ctgaccgccg
     1081 ccgccatccg ggccacgccg ggcgtcctca tcgtgccgct cgagcaggcc ttcggctgga
     1141 gccgggccac gatctcgttc gcggtctcgg tcaacctcct gctctacggc ctctgcgggc
     1201 cgttcgccgc gtcgctgatg gagcgcttcg gggtgcgccg ggtgacgctc ctgtccctct
     1261 gcgcggtgac cgtcggggtg gccctcacca ccgtgatgcg gtcgccgtgg cagctcgtcc
     1321 tcctgtgggg cgtggtcgtc gggatcggga ccggcgcgat ggcgatggtg ctggccgcca
     1381 ccatcgcgaa ccgctggttc gtggcccggc gcggtctcgt catcggcatc ctcaccgcga
     1441 gcaccgccac cggccagctg ctcttcctcc cgctgctcgc cgcggtggtg cagcgcaccg
     1501 gctggcgcgc ggccgccctc gtggtcaccg gggtggccct gctcctggta ccgatcgtgg
     1561 tgatcttcat gcgcgaccag ccccgcgacc tcgggctccg cgccttcggc gacacgccgg
     1621 aggcggtgag cccggctcgc ccggcgacga ggcccgccgg cgcgatcgcc gccctcgtcg
     1681 agggatcgcg gtcgcgggac ttctggctgc tggccggcac cttcttcatc tgcggcctca
     1741 gcaccaacgg actgatcggc acccacctga tcccggcctc gatggaccac gggattcccg
     1801 cggtgaccgc ggcgggcctc ctggccgcga tgggcgtctt cgatctcatc ggcaccaccg
     1861 tctccggctg gctctcggac cgctgggaca accggtggct cctcttcggc tactacggcc
     1921 tccgcgggct ctcgctcatg gtgctgccct acgcgtacgg gtcggagtac ttcggcctca
     1981 ccctcttcgc ggtgttctat gggctcgact gggtcgcgac ggtgccgccg accgtccgcc
     2041 tgaccgagac cctgttcggc aagcatcggg tcggcatcat gtacgggtgg atcgcggtcg
     2101 cgcaccagat gggcgcggcc accgcggcct tcggcgccgg tctcctgcgc acctggttcg
     2161 gctcctacca ggtggccttc accggcgccg ggttcgtctg cctcatcgcg gccctgatgg
     2221 tcctggcgat cgggcacggg cagatcgtca ccgggctgcg ccggcggcgg gtggagatct
     2281 ccggcgcggt cgagggctag cgccggccgg acgcggccgc ccgcagcgcg gggcgggcca
     2341 gccggaagag ctccacgttc caccgcgtcc gccccgcggt cgccaggtcg gccatgatct
     2401 ccccgaccac gctgcagaac ttgaacccgt ggccggagaa cccggccgcg atcgaaactc
     2461 gaggcgcgtc cggatggacg tccaggatga agtgctcgtc cggggtgttg gtgaacatgc
     2521 acgcggccat cgagagcgtg ggcccgtccg cgtccgggaa gtagcgccgg atcccctcgc
     2581 gcaccaccgc ctcgtcgcgg gcgtcgcagt cgcggtcgat cgaatcggga tcgacctgtt
     2641 gctcgcggtg atggtacttg ccgatcttga agccggggat cccgtggatg gggaatccgt
     2701 agaagcgtcc ctccggcgat tccatgttga acaccggaaa ggccccgacc cggaagcgct
     2761 ccggccgccg gggctgcgtc cacagcatga cctgccgctc gggcttcgcc accggggcca
     2821 gcgcgccggc cagcttgccg ctccaggcgc ccgcggtcag gacgagccgc tccgcttcgt
     2881 agctcccccg gtccgtccgc acccggactc ccccgcggcc caccttccag tcgagcaccc
     2941 gctcgcgccc gtggatctcg gcgccgtggg ccaaggcgcc gttggcgtgc gccaccacac
     3001 tccgctccgc catcacgaat ccggcgtccg cctggtacac ggagaccatg ccccgcgcga
     3061 gccggtagcc cggaaagcgg cgcccgaccg cggccgcgct catcaactcg tgggggatcc
     3121 cgtgctcgtg acacgagcgc acggagccgg cgaccagcgt gccgtcctcg ggccccacgt
     3181 cgagcccgcc ggtgacgacg aggagccgct cgccggtgga gttctccagc tcgcgccaca
     3241 gctcgtaggc ccgccgcagc agcggcacgt agctcggatg ctccgcgtac gcgagccgga
     3301 tgatccggtt gacgccgtgg gaggagccga gctggtgggg aatgtcgaac tgctcgatgc
     3361 ccaggacctt cttcttccgg cgggccaggt ggtaggccgt cgcgctaccc atgcctccga
     3421 ccccgatcac gatcacgtcg aacgccaccg tctcgccccc tcccgcgctc gcgcgccgat
     3481 cgtcccgcac tacccgggtc gccgcccggt cacccgcgtg agcacgccgg ccacgaactc
     3541 gtggacggcc acgtcgcgcg cgtccgcgcg ctcgaagtag gcgcggcact ccgccaccga
     3601 gtgggcgaca ccggtgctgc cccagagggc ttcggccagg ccccagagcg cgggatcgcg
     3661 aggcccgccg cgcccgtcgt cgagcatctc gccgatcagg tgcatctccc cgccgggcgt
     3721 gagcgccgcg aaggcgcgcg tgatcacggc ctggatgatc tcgcggctgt actgcggcag
     3781 gttgctcgcc atgatgacga cgtcggcgtc ggccgggagc gggtcccgcg tgaagtcgcc
     3841 cgcctgggcg gtgacgcggt cggccagccc gtgggaggcg atgaactcgc gcgtcacctc
     3901 cacgaccggc ggcaggtcga agacgatggc gcgcagctcc gggtgctgct gcgcggccac
     3961 gatgcagtag cagccggagc cgccgcccag gtcgaggatc tgccgccggc ccgcgaggtc
     4021 cacctgccgg acgaatcgcc gagccgcgcc catgccgatc gagtaggtcg cctcgtggta
     4081 cctcctcgcc tgctccaccg tgaagccgtg ggcgtacttg ccgagcacga ccgagtcgcg
     4141 acggccgagg tggccggcga gccgccccca ctcgttccag tccggcttgc tgaagagcat
     4201 ccacggcccc gcataggtgg gctcgccctc gacgaggaac cgggccacgt cgggcgcgtt
     4261 gcgcacgcga tcgccgtcgc gcacgagcag gcccagggcc acgcacgcgg tgaccagccg
     4321 ctccgcgttg gtgggcgtca ggtcgagcgc ccgagctacc gcggcctcgg tctccgcccc
     4381 gcgcgcgacg cgggtgaaga gacccagctc caccgcggcc atgagggcgg cggagtcgcg
     4441 gtaggaatgc gcgaggcgct gcaggcgcgt ggtatccggt cggggcgtgg gcacgttcaa
     4501 ggggcggcgg tcctcggcgg cggcattatg gcacgccccg cggcccgaag agctctcgtc
     4561 gagtgccctg gatcgtgggg gactgcgaga caatgttccc cggaggggac tcacgcatgc
     4621 cgggaatcga ccgccgacgg ttcttcaacc tggtcggccg gaatgggggc gcggccgcgc
     4681 tctacagcac cctgggtgcg atggggatcg tcgctccctc gaccccctac cgcgccctga
     4741 ctcgcccgcc gaaggattcc ggccgaggca tccggatcgc ggtgctcgga gccggcatcg
     4801 cggggatgtc ggccgcctac gagctccaga gagccggcta cgagtgcacc gtcctcgagg
     4861 cgcgtgaccg gccgggggga cgcatctgga cgatccgctc gggggacacg atcgcggaga
     4921 cggattcgag ccagcgggtg gcctgggatc ggcagcccga tctctacctg aatgcgggcc
     4981 cggcccgcat ctcacagcac catcaggcga tgctctccta ctgccgcgag ctccgcgtgc
     5041 cgctggaggt gctcgtgaac gacaatcggg cggccctgct ccaggacgac gccgcgttcg
     5101 gcggagagcc ccaggtcgcg cgccgcgtgc tcgccgatac acggggcggg atcgcggcgc
     5161 tggcggccgg cgcgctcact cccggggcgc gccagtcccg gtccgatcgt aatttgcgga
     5221 ccctgctgca ggggttcgga gccctcgacg acgacctgcg ctacgccggt tccgtccggg
     5281 ccggctacgc ggagcccccg gggctgactc cggggcagcc cggacgccgc cacccggcgc
     5341 tcccgctcgc ggagatcgcc cgggccgcct cgtggccgtc ggcgatggcc gccgctgaaa
     5401 cgtgggatca ggcggccacc atgctgcagc cggtcggcgg gatggacgcg atcgctcgtg
     5461 ccttcgcacg ggctctgggc acgaccatcc ggtaccaggc ggaagtggtc gggatccgtc
     5521 gcatcggcga ccgcgcccgg gtgtcctggc gggatcggaa gcagggctcg cctcacgctc
     5581 tggacgcgga cttcgtcatc tgcaccattc cgctgccggt gctgagcggc atcgagtccg
     5641 actttccgga ccccgtgaag cgggccatcg aggtgggcgc cctcgcctac atcccggcgg
     5701 tcaaggtggc gttccaggcg aatcgacgct ggtgggagac cgaccagcag atctacgggg
     5761 gcatcagctg gaccagtcgc gacatcaccc agatgtggta cccctcggcc gggctcaacg
     5821 gggacaaggg gatcctgctc ggcgcgtaca tctggacgac atcgatcggc aggcggttcg
     5881 ccgcgatgag cccggcgcag cgactcgcgg ccgcgatcgc cgacggcgag cggctgcacc
     5941 ccggctacgc gggcctggtg gacaagggcg tgtccatcgc ctggtcgaag atcccgttca
     6001 cgggcggcgg ctgggtggac tggggaaatg cggcctggcg cgccgcctat cccgctctgc
     6061 tgaatggcca cggcgcggtg cacttcgcgg gagagcacat gagctacatg aacagctggc
     6121 aggagggcgc ggtgcgctcc gcccacgaca tcgtcgagcg aatcgccgcg cggaagcgtg
     6181 accgagcccg ctgagggccg atccgccgcg cggcgcccgc ctcagctcgc ggcctgggcc
     6241 gggcccgttc cggtcggcag cgtcatgccg gacggcgcct cccgcatcgc gaccggccgc
     6301 ctcaccgcgg cggaccgagc ggccgccagc accgcgacca gggcctgccg gccgtcatgg
     6361 agcgtggcct ccatgggcgt tcccgtccgg atgctacgcg cgaactgccg ccactgatcg
     6421 cagtacgaga ctcgatagtc tcccgcccgg tgcatgcgcg gcagggcgcg aggcagctcc
     6481 ctcaggaagt ggcccatgcg tccgagccgg gccggcaggc tgctgggcat cgtctcggtc
     6541 gggtagtact cgaggccctc gaagcggatc agggagaccc gcaggcgccc cgccttgccg
     6601 cagatctcga actcgagatc gtgattggcg cgctcggaga acaccgccga caccagcacc
     6661 ccgttgctca tgtgcccgga gacgaccgcg gcctcgtcct cccaccggcc gtcgaccgtc
     6721 gtcgcgaaca gctccgcgat ctcggcgccg agcagatagc gccagaggtc gaagtggtcg
     6781 ctcgcgacct cgacgagggc cccgccgccc tgcgcgcgat ggcgccgcca ctccggcagc
     6841 gtgtcgtcgg cgcggggact gttccaggtc gcacggatgg tctggatcgg ccccagatct
     6901 ccgcggtcga cgatctcctt ggcctggcgg acgagacggt gccagcgcat gtggaagccg
     6961 accgtcacct gccgcccgga ggcgcgggcc ttcgcgatca gccgatcgca gtcggggagg
     7021 gaaaggccga tcggcgcctc gatccacacg tgcttgtccg cctcgagcgc ggcgagcgcc
     7081 gccgcgacct gcagccgggg cggcaggcac accgcgacga cgtcgatctc gcggtcggcg
     7141 aggagcgacg cggcacctcg atggaggcgg gggatcccgt accgcgcctc gaccgaccgg
     7201 agccgctcgg gatcgacgtc ggccaccgcc acgacctcga gctcccgcac ttcgcgcacg
     7261 gcgggcagat gcttctcggc cccgacctcg ccacatccga tgaagccaac cctgagtgga
     7321 cgctccattc ccctacctta ggggatgccg aattttttgg ggccgattat gtgcgctaga
     7381 gcccggactc ccgccactcg ggggcgggct ccgggggatc gcacccgagg ccggccgcct
     7441 tcacgccctc gatggcgcag tactcgtcgc gatcggacgt atcgccgctg atgccgaccg
     7501 ccccgatggc ccggccgtcg cggtccagga tgaggacgcc ccctggcacg gggatgaact
     7561 gcccctgcga ggcggtggcc accgcgctct ggaacgcggg gcggttctgc agccggtccc
     7621 ggatcgtgcg ggacgggatg cccatcccga gcgcccctcg ggccttgccc tgggcgatcc
     7681 gcacccgaag ggtgccgcac ccgtcgtcac gctggagcgt gacgagctgc cctccgccgt
     7741 ccacgacgac gacggcgagc gggagcatct tctcgcgccg gccggccgcg atggcgcggg
     7801 tggcgatggt ggtggcctgc tccagggtga gggtcgagac gacgtggcga aggaccatgg
     7861 cggatcgctc ctttctcggt cgacggcgcg cgctaccgcg ccggctcgcg ggccgcgcgg
     7921 cggctctcct cgcggatcag gccgagcacc tcccgtttga gctcgttgaa gcgcggcgag
     7981 gtagtcgcct cgagggtccg ggggcgcggc aggtccaccg tgagccgcgt cttgatgcgc
     8041 ccgggccgcg cggtcatgac gcagacagtg tcggcgagga gaagtgcctc ctccacgtcg
     8101 tgggtgatga acaggaccgc ct
//
LOCUS       DATMCV010000588         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_29041, whole genome shotgun sequence.
ACCESSION   DATMCV010000588 DATMCV010000000
VERSION     DATMCV010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29041"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VNU03_13610"
     CDS             complement(<1..654)
                     /locus_tag="VNU03_13610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP75552.1"
                     /translation="MRRRDRRAGAAGTRAVRLGYKLSSEERGPRELVRLARQAEETGF
                     GFALISDHFHPWTDRQGQSPFVWSVIGGIAQVTRTLALGTAVTCPSVRIHPALVAQAA
                     ATCALLMEGRFFLGLGTGENLNEHIVGQGWPETEVRQERLLEAVQVIRLLWGGGNVSH
                     RGRHFTLENARLYSRPDTPPPLLLAVGGPRTADLAGSLADGMIGTDPDPDLTTRFARA
                     "
     gene            complement(651..>2428)
                     /locus_tag="VNU03_13615"
     CDS             complement(651..>2428)
                     /locus_tag="VNU03_13615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP75553.1"
                     /translation="TPDAPERATETRYRRPDGSDRWVDASLSLVHGADAPASGAADSV
                     TIVALDATARRAALDAARAGDTRFRTMADAAPVLVWLAAPDARRTWFNSEWLAFVGSR
                     LDEEIGAGWLAHVHPDDRARCTEAATRDYAARRRCRLVYRLRRHDGMDRWVHDDGVPL
                     LAADGTFTGYVGACVDVTGEKLAELERRELLERERKARREAEAASQAKDEFLAALSHE
                     LRTPLHALRLWAGILRNGLHDPQALARAADTIDRNAVLQSRLVDDLLDVSRIVSGQLR
                     LTIEWVAIGPIVEAAVETVRPAALNKGVALIQDLGPGVGSVRGDAIRLQQVVWNLLAN
                     AVRFTPAGGRVEVRLRPVDGAAELSVTDSGRGIGAGLLPHVFDRFRQGESGTNRSHGG
                     LGLGLSLARQLVELHGGTIEAASRGPGRGATFTVRLPVARADPPPASPASGSGASGSA
                     RSLEHVRVLVVDDDAETREVMTVALGREGAIVTTAPSVGEALAIIEHGWPDVLLSDIG
                     MPGEDGYDLIRKIRHLEALGGRHLPAIALTGYAADDDRRRVIEAGFDAHVAKPVPAAT
                     MAPLIATLLEREGGLPHNQPGDLPT"
BASE COUNT          310 a          922 c          881 g          315 t
ORIGIN      
        1 ggcccgggcg aagcgcgtgg tcaggtccgg gtccggatcg gtcccgatca tgccgtcggc
       61 cagactcccg gccaggtcgg cggtgcgggg gccccccacc gcgagcagga gcggcggcgg
      121 cgtgtcgggc cgcgagtaga gccgcgcgtt ctcgagcgtg aagtgccggc cgcgatggct
      181 gacgttcccc ccgccccaga gcagccggat cacctgcacc gcctcgagca gccgctcctg
      241 ccgcacctcg gtctcgggcc agccctgccc cacgatgtgc tcgttgaggt tctccccggt
      301 gcccagcccc aggaaaaacc gcccctccat cagcagggcg cacgtggccg ccgcctgggc
      361 gaccagcgcg ggatggatgc gcacggaggg gcaggtcacc gcggtgccca gcgcgagggt
      421 gcgggtcacc tgcgcgatac cgccgatgac gctccacacg aacgggctct gaccctgccg
      481 gtccgtccag gggtggaagt gatccgagat gagcgcgaat ccgaagccgg tctcctcggc
      541 ctgccgggcc aggcgcacca gctcgcgcgg cccgcgctcc tcgctcgata gcttgtagcc
      601 gagccgcacc gcgcgggtcc ccgcggcgcc cgcccgtcga tctcgccttc tcatgtcggg
      661 aggtctcctg gttggttgtg gggcaacccg ccttctcgtt cgagcagggt cgcgatgagc
      721 ggcgccatcg tggcggccgg caccggcttg gccacgtgcg catcgaagcc ggcctcgatc
      781 actcgccgcc ggtcgtcgtc ggccgcgtac ccggtcaggg cgatcgccgg aaggtgccgg
      841 ccgccgagtg cctcgaggtg gcggatcttg cggatcaggt cgtatccatc ttccccgggc
      901 atcccgatgt cgctcagcag cacgtcgggc cagccgtgct cgatgatcgc gagcgcctct
      961 ccgaccgagg gagcggtcgt cacgatcgcc ccttcccggc cgagggccac cgtcatcacc
     1021 tcgcgggtct cggcatcgtc gtccaccacc agcacgcgga cgtgctcgag ggaccgcgcg
     1081 gagccgctgg ccccggaccc gcttgcgggt gaggcgggcg gcgggtcggc acgcgccacc
     1141 ggcaggcgca ccgtgaaggt ggcgccgcgc cccgggcccc ggctcgcggc ctcgatggtc
     1201 cccccgtgca gctcgacgag ctggcgggcg agggagagcc cgaggccgag cccgccgtgc
     1261 gaccggttgg tcccgctctc gccctggcgg aagcgatcga agacgtgggg cagcaggccg
     1321 gccccgatgc cacgacccga atcggtcacg gacagctcgg ctgccccatc caccggccgg
     1381 agccggacct cgacccggcc gcccgcgggc gtgaagcgca ccgcattggc caggagattc
     1441 cacaccacct gctgcagccg tatcgcgtcg ccccgcaccg accctacccc gggaccgaga
     1501 tcctggatca gcgcgacccc cttgttgagc gcggcgggcc ggaccgtctc gaccgcggcc
     1561 tccacgatcg gtccgatcgc cacccactcg atcgtgagcc ggagctggcc cgagacgatg
     1621 cgggagacgt cgagcaggtc gtcgaccagc cgcgactgca ataccgcgtt gcgatcgatc
     1681 gtgtcggcgg cgcgcgcgag ggcctgcggg tcgtggagcc cattgcgcag gatgccggcc
     1741 cagagccgca gggcgtgcag cggcgtgcgc agctcgtgcg agagggcggc caggaactcg
     1801 tccttggcct ggctcgcggc ctccgcctcc cgccgcgcct tccgctcgcg ctcgagcagc
     1861 tcgcggcgct cgagctcggc cagcttctcc ccggtcacgt ccacgcaggc gcccacgtat
     1921 ccggtgaagg tcccatcggc ggcgaggagc gggaccccgt cgtcgtggac ccagcgatcc
     1981 atcccgtcgt ggcgccgcag gcgatacacg agccggcagc ggcgccgggc cgcgtagtcc
     2041 cgcgtggcgg cctcggtgca gcgcgcgcgg tcgtcgggat gcacgtgggc gagccagccc
     2101 gcgccgatct cctcgtccag ccggctcccc acgaaggcga gccactccga gttgaaccag
     2161 gtgcgccgcg cgtccggagc cgccagccac accagcaccg gcgccgcgtc cgccatcgtc
     2221 cggaagcgcg tgtccccggc ccgggccgcg tcgagggcgg cgcgacgcgc ggtcgcgtcg
     2281 agcgcgacga tggtgacgga gtcggccgcg cccgaggccg gcgcgtcggc gccgtgcacg
     2341 agggagaggc tcgcgtccac ccagcggtcc gatccgtcgg gccggcggta gcgggtctcg
     2401 gtggcccgct cgggtgcgtc cggggtaa
//
LOCUS       DATMCV010000589         2949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_899257, whole genome shotgun sequence.
ACCESSION   DATMCV010000589 DATMCV010000000
VERSION     DATMCV010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899257"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1978)
                     /locus_tag="VNU03_13620"
     CDS             complement(<1..1978)
                     /locus_tag="VNU03_13620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWP75554.1"
                     /translation="MTGRAAVALAALLLGLAAVMAVWLSIDRRPPEWDHANHLERAVL
                     CQRSLAEPGREALQAIIAESSFYPPIVTCAAGLLGLALPPLAAAHAVMWGFLVVGTLA
                     VFGLGRRLLDPAAGLLAAFLFATAPFVVFSLLHFQLDLPLAALVAVTLYALAHPEALA
                     GPRGALGLGVLLGLGMLTKPPFAAYVSGALLWAAWLAGRAPDRRARLGRLLAAVGVAA
                     LIALPWYGPRLVGLPMQVLNRSFKQAAESGHAPALTSGALLFYPRWIVPQLGLLATLL
                     LLGGMVAVARRERARGLIWAALAPAVIFFLIQNKNLRYTLPLLPAAALAGAAAVAALA
                     PRARAAVTLACLAVGVLQVGSAAFALPSPTALAPVVPTLAFSQPPDGRDWHHRDLLAA
                     ITAAAAGPGEPRVAVVANDNFFSVSNFRYEVVRDRLPLRLSRAWDQAPMGVDFAIVKT
                     GDQGPDAASAKPDRIMAALGGGDPWLAAAYPVIARVPLPDGSEGMVRMRRLEPVRALA
                     PAELAERLRRGAEGLLADVVRDARGLRMTLDYRDEALLAGQVDHVTLEAESALVGEFT
                     RRKPPLRVGPVRIRASGVLVNPGRLAATGTIELLDVRSLAVERLGVTEEDLAEFLARQ
                     RGLSGLAATVERCGIGVHWSLPGPDLRARLGLSAG"
     gene            complement(1975..2715)
                     /locus_tag="VNU03_13625"
     CDS             complement(1975..2715)
                     /locus_tag="VNU03_13625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP75555.1"
                     /translation="MDPAYAAVHAEEDRAHWWFQGRRAIILAELARLLPGGGRRVAEI
                     GCGSGGLLEALKGLGTAVGVEMDPALRGIARARGLDVRAGSLPDDIPLEAGRWDAACL
                     FDVLEHVTDEAGALRACRRLLAPGGRLVVTVPAYAWLWSRHDELLGHRRRYTAGSLRA
                     VVEAAGFAVDRVTYFNTLLAPPIMAMRVVRAALRRSSHDLERPAPLVNRLLARAFGAE
                     ATLLRFVSLPFGISILLVAHVAEPAPGP"
     gene            complement(2718..>2949)
                     /locus_tag="VNU03_13630"
     CDS             complement(2718..>2949)
                     /locus_tag="VNU03_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWP75556.1"
                     /translation="SRVAEALAGVFRPVKMNYELLGNQVPHIHWHLVPRLPTDPDLLS
                     PIWRVDHQPAPLAPATARERIESIRRALDGAA"
BASE COUNT          443 a         1092 c         1145 g          269 t
ORIGIN      
        1 gccccgcgct gagcccgagc cgggcccgga ggtcggggcc gggcagcgac cagtggacgc
       61 cgatcccgca ccgctcgacg gtggccgcga gccccgagag gccgcgctgc cgggcgagga
      121 actcggcgag gtcctcctcg gtgaccccga gccgctccac cgcgagcgag cgcacgtcga
      181 gcagctcgat cgtcccggtg gcggccagtc ggcccggatt gaccagcacc ccgctcgccc
      241 ggattcgcac cggcccgacg cggaggggcg gcttgcggcg cgtgaactcg ccgaccagcg
      301 ccgactccgc ttccagcgtc acgtgatcga cctgacccgc gagcagcgcc tcgtcccggt
      361 agtcgagggt catccgcagc ccgcgggcat cgcgcaccac gtcggccagg agcccttcgg
      421 ccccgcgccg gagccgctcc gcgagctcgg cgggcgcgag cgcgcgaacg ggctcgaggc
      481 gccgcatccg caccatgccc tcgctgccgt ccggcagcgg cacgcgcgcg atcaccgggt
      541 acgccgcggc caaccagggg tccccgcccc cgagggcggc catgatccgg tcgggcttgg
      601 ccgaggccgc gtccggtccc tggtcgccgg tcttgacgat cgcgaagtcg acgcccatcg
      661 gagcctggtc ccaggcgcgc gagagccgga gcgggagccg gtcccgcacc acctcgtagc
      721 ggaagttgga caccgagaag aagttgtcgt tggccaccac cgccacccgg ggctcgccgg
      781 ggccggctgc ggcggcggtg atggcggcga gcaggtcgcg atgatgccag tcgcggccgt
      841 cgggcggctg cgagaaggcg agcgtgggca cgacgggcgc cagcgccgtc ggcgacggaa
      901 gcgcgaacgc ggccgagccg acctggagca cgcccaccgc gaggcaggcc aacgtgaccg
      961 cggcgcgcgc gcgcggagcg agcgccgcca ccgcggccgc cccggccagc gcggcggcgg
     1021 gcagcagggg cagcgtatag cgcaggttct tgttctggat gaggaagaag atcaccgcag
     1081 gggccagcgc ggcccagatg aggccgcggg cccgctcccg ccgcgcgacc gcgaccatgc
     1141 ccccgagcag cagcagggtg gccaggagcc ccagctgcgg gacgatccag cgcgggtaga
     1201 agagcagggc gcccgaggtg agcgccggcg cgtgacccga ctcggcggcc tgcttgaacg
     1261 agcgattcag aacctgcatg ggcagcccga ccaggcgcgg gccgtaccac ggcagtgcga
     1321 tcagcgcggc caccccgacc gcggccagca ggcgtcccag gcgggcgcgc cggtcgggcg
     1381 cgcggccggc gagccacgcc gcccacagca gcgcgcccga cacgtaggcc gcgaacgggg
     1441 gcttcgtgag catgccgagc cccaggagca cgccgagccc gagggcgccg cgcgggccgg
     1501 cgagcgcctc gggatgcgcg agcgcgtaga gggtcacggc caccaacgcg gcgagcggga
     1561 ggtcgagctg gaagtgcagg agcgagaaga ccacgaaggg cgcggtggcg aagaggaagg
     1621 cggccaggag cccggcggcg ggatcgagga ggcggcgccc gagcccgaac accgcgagcg
     1681 tgcccaccac caggaagccc cacatgaccg cgtgcgccgc ggcgagcggg ggcagcgcca
     1741 ggccgagcag acccgccgcg cacgtgacga tcggcggata gaagctcgac tccgcgatga
     1801 tcgcctgaag cgcctcccgg cccggctcgg cgaggctccg ctggcagagc acggcccgct
     1861 cgaggtggtt ggcgtgatcc cactccggcg ggcgccgatc gatggagagc cacaccgcca
     1921 tgaccgccgc cagccccagg aggagcgcgg ccagagccac cgcggcgcgg ccggtcatgg
     1981 gccgggcgcg ggctcggcga cgtgggcgac cagcaagatc gagatgccga acggcagcga
     2041 gacgaagcgc agaagggtcg cctcggcgcc gaaggcgcgg gccagcagcc ggttcacgag
     2101 tggcgcgggc cgctcgagat cgtggctgga ccggcgcagc gccgcccgca cgacgcgcat
     2161 cgccatgatc gggggcgcga gcagcgtgtt gaagtaggtg acccggtcga ccgcgaagcc
     2221 cgccgcctcc accaccgcgc gcagcgagcc cgcggtgtag cggcgccggt ggcccaggag
     2281 ctcgtcgtga cggctccaga gccaggcgta ggcgggcacc gtcaccacca ggcgaccgcc
     2341 gggcgcgagc aggcggcggc acgcccgcag ggcgcccgcc tcgtcggtga cgtgctcgag
     2401 cacgtcgaag aggcacgccg cgtcccaccg ccccgcctcg agcgggatgt cgtccggcag
     2461 cgagcccgcc cgcacgtcga ggccgcgcgc tcgcgcgatc ccgcgcaggg cgggatccat
     2521 ctcgaccccg accgcggtcc cgaggccctt cagcgcctca agcaggccgc cgctgccgca
     2581 gccgatctcg gccacccggc ggccgccgcc cggcaggagg cgggccagct cggcgaggat
     2641 gatcgcgcga cggccctgga accaccagtg ggcccggtcc tcctcggcgt gcaccgcggc
     2701 gtaggcggga tccacggtca ggccgctccg tcgagggcgc gccggatgga ctcgatccgc
     2761 tcgcgggccg tcgcgggagc gagcggcgcc ggctgatggt cgacgcgcca gatgggcgag
     2821 agaagatccg ggtcagtggg caggcgcggg acgaggtgcc agtggatgtg cggcacctgg
     2881 ttgccgagca gctcgtagtt catcttcacc ggccggaaga cgcccgcgag cgcctcggcc
     2941 acgcgactc
//
LOCUS       DATMCV010000590         2377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_229433, whole genome shotgun sequence.
ACCESSION   DATMCV010000590 DATMCV010000000
VERSION     DATMCV010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229433"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VNU03_13635"
     CDS             <1..330
                     /locus_tag="VNU03_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020380265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75557.1"
                     /translation="PSGAFYNERYAAVTFRFVNNNDVVTRVPPRELSYDHVDFRRYFD
                     SHGQLVADMRLLDALRDSFEGSILGLRKLFEELARQQDQRPALPLPDFLDDDRIDNYI
                     ACLGKSA"
     gene            complement(341..1828)
                     /locus_tag="VNU03_13640"
     CDS             complement(341..1828)
                     /locus_tag="VNU03_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWP75558.1"
                     /translation="MSGGARAGSALAVIVTALAAGCSVGPDYVQPPVITPDAYKENVG
                     WKVAEPRDDVLRGTWWQLFGDPTLDALEEQVSLGNQDLVVAEATYRQARALVRQARAA
                     YFPTVTAGVGYTRSRQSDTLGSPSGSSSSGPGGRGNAASNQPVSTFQLPIEVAWTPDF
                     WGRVRRAVESGRASAQASGGDLETARLSFQAELAQDYFQLRMLDAQKRLLDDTVAAFT
                     TALQLTQNRRASGVASRVDVVQAETQLKTTRALAIDVGVARAQTEHAIALLLGQPAST
                     FSLPVAPLASTPPPIPIGVPSALLERRPDVAAAERRMAAANAQIGIAVAAYYPTVTLS
                     FSGGLESSSVSQWFTWPSRFWSVGPSITETVYDGGLRKAQTDQARAAFDGSVAAYRQA
                     VLTAFQGVEDNLAALRILEEEARVQDEAVRAAQESVTLTTNQYKAGVVSYLNVITVQT
                     IALNDEITAVQLHGRRMAAAVLLIQALGGGWTTDELPSAKAASAP"
     gene            complement(1825..>2377)
                     /locus_tag="VNU03_13645"
     CDS             complement(1825..>2377)
                     /locus_tag="VNU03_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP75559.1"
                     /translation="LANESILILAAIVTVYIVLGMLYESFIHPITILSTLPSAGVGAL
                     LALMMFRIEFSLMAMIGVLLLIGIVKKNAIMMIDFAIDAERREGKAPAEAIYQACLLR
                     FRPIMMTTMAALLGAVSPALGIGEGAELRQPLGISIVGGLILSQILTLYTTPVIYLYL
                     DRFRLWWRGRVTRRSAALEGARP"
BASE COUNT          348 a          822 c          864 g          343 t
ORIGIN      
        1 ccctccggcg ccttctacaa cgagcggtac gccgcggtga ccttccgctt cgtgaacaac
       61 aacgacgtgg tgacccgcgt cccgccccgc gagctgtcgt acgaccacgt cgactttcgc
      121 cgatacttcg acagtcacgg ccagctcgtg gccgacatgc gactcctcga cgcgctccgg
      181 gacagcttcg aggggtcgat cctgggtctg cggaagctct tcgaggagct tgcccgccag
      241 caggaccagc gcccggcatt gcccctgccc gacttcctcg acgatgaccg catcgacaac
      301 tacatcgcgt gcctgggcaa gagcgcgtga cggtccggga tcagggcgcg gaggccgcct
      361 tcgcggatgg cagctcgtcg gtggtccagc cgccgccgag ggcctggatc aggagcaccg
      421 ccgcggccat gcgccggccg tggagctgca ccgcggtgat ctcatcgttc agcgcgatgg
      481 tctgcaccgt gatcacgttg aggtagctca ccacgcccgc cttgtactgg ttggtggtca
      541 gcgtcaccga ctcctgcgcc gcccgcaccg cctcgtcctg cacccgcgcc tcttcctcga
      601 gaatgcggag cgcggccagg ttgtcctcca cgccctggaa ggcggtgagc acggcctgac
      661 ggtaggcggc cacgctgccg tcgaaggcgg cgcgcgcctg gtcggtctgg gccttgcgga
      721 gcccgccgtc gtagacggtc tcggtgatgg acggccccac cgaccagaag cggctcggcc
      781 aggtgaacca ctgcgagacg ctgctgctct cgaggcctcc cgagaagctc agggtcaccg
      841 tcgggtagta ggccgcgacc gcgatgccga tctgggcgtt ggcggcggcc atgcgccgct
      901 ccgccgccgc gacgtccggc cgccgctcga gcagcgcgga gggtacgccg atcgggatgg
      961 gcgggggcgt gctggcgagc ggggcgacgg gcagcgagaa ggtggacgcg ggctgtccga
     1021 ggagcagggc gatcgcgtgc tcggtctgcg cccgggccac ccccacgtcg atggcgagcg
     1081 cccgcgtcgt cttgagctgg gtctcggcct gcacgacgtc gacgcgggag gccactccgc
     1141 tcgcgcggcg gttctgggtg agctgcagcg cggtggtgaa cgcggcgacc gtgtcgtcga
     1201 gcagccgctt ctgggcgtcg agcatgcgga gctggaagta gtcctgggcg agctcggcct
     1261 ggaagctcag ccgcgcagtc tccagatctc ccccgctcgc ctgggccgac gcccgtcccg
     1321 actccaccgc gcgccgcacc cggccccaga agtcgggggt ccacgccacc tcgatcggga
     1381 gctggaaggt cgagaccggc tggttcgagg ccgcgttgcc cctgccgccc gggccggagg
     1441 acgacgagcc cgacgggctg ccgagggtgt ccgactgccg ggagcgcgtg tagcccaccc
     1501 cggcggtcac ggtggggaag taggccgcgc gcgcctgacg caccagcgcg cgggcctggc
     1561 ggtaggtggc ctcggccacc accagatcct ggttgccgag gctgacctgc tcctccagcg
     1621 cgtcgagtgt cgggtcgccg aagagctgcc accacgtgcc gcgcaggacg tcgtcccgtg
     1681 gctcggcgac cttccagccc acgttctcct tgtaggcgtc cggcgtgatc accggcggct
     1741 gcacgtagtc ggggccgacc gagcacccgg ccgcgagcgc ggtcacgatg actgcgaggg
     1801 ccgatccggc gcgcgcgccg ccgctcacgg ccgcgccccc tcgagggccg cggagcggcg
     1861 cgtgacgcga ccgcgccacc agagccggaa gcggtcgagg tagagataga tgaccggagt
     1921 ggtgtagagg gtgaggatct ggctgaggat caggccaccc acgatggaga tgccgagcgg
     1981 ctggcgcagc tccgcgccct cgccgatgcc gagggcgggg gagaccgcgc cgagcagcgc
     2041 ggccatcgtg gtcatcatga tggggcggaa gcggagcagg cacgcctggt agatcgcctc
     2101 cgcgggggcc ttgccctccc ggcgctcggc gtcgatcgcg aagtcgatca tcatgatcgc
     2161 gttcttcttg acgatgccga tcagcagcag gaccccgatc atggccatga ggctgaactc
     2221 gatgcggaac atcatgaggg ccagcagggc ccccaccccg gcggagggca gcgtcgagag
     2281 gatcgtgatc gggtggatga agctctcgta cagcatgccg agcacgatgt acacggtcac
     2341 gatggcggcg agaatcagga tcgactcgtt ggccagc
//
LOCUS       DATMCV010000591         2962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_765098, whole genome shotgun sequence.
ACCESSION   DATMCV010000591 DATMCV010000000
VERSION     DATMCV010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765098"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VNU03_13650"
     CDS             <1..630
                     /locus_tag="VNU03_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWP75560.1"
                     /translation="PLIVGTNLEEMKLYRFLDPLLERLDEAALLQRCGLLFPGTGPDG
                     RTHAERAVDTYRSARRARGEDASAVETWLAISTDHVFRAGALKLAELHARHTPEVFVY
                     QFEWKGKEPGRPQGAIHALELPFVFGTLATSEIGAIAGRTPAAQTLSGQMQDAWIAFV
                     RGGRPRAAGLPEWPPYAAPRRATMVLADPSGLVDAPGEPERAVWDAFLG"
     gene            complement(644..1273)
                     /locus_tag="VNU03_13655"
     CDS             complement(644..1273)
                     /locus_tag="VNU03_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP75561.1"
                     /translation="MTLAHGTDRLESARLVLRRVAPDDLPFFTRIHALPEVAQHLYPA
                     GRPRSPEETAGWLRYTLASYEQLALGYLAVVRKADGALIGRCGLMDLVVESAAPDHGI
                     RRGWFGREEAPADVALTFEVELGYTLDPAVWGQGFATEAARCVRDYARDVLRLPYAIS
                     AILPLNARSRRVAERSGARATGQMDVVGLTWDRYVWPLATGETSRERET"
     gene            complement(1287..2294)
                     /locus_tag="VNU03_13660"
     CDS             complement(1287..2294)
                     /locus_tag="VNU03_13660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75562.1"
                     /translation="MKFGLYSSIANPPRGEQLDRCIDEVIAEAQLAEASGFDSCFFGE
                     HHQDHDGFLPSPLIVATAVAARTTRLRVGTSVILLPLHHPVHVAEDVITLDLVSKGRV
                     ILGVGIGYQPADFRAFSVPMEDRAGRFEESIEILRRCWAGERFSFHGKHYTLEDVQIR
                     PRPYQKSGPPLWIGASIDAAARRAGRIADGFVGTPSTGLANATHLADVYKEAAREAGR
                     PAEVVQMRDAWVARSRAEADAVYGPHVMAAYRYYWENRLAEFRNLSAGSEFTLDSLAP
                     DRLILGDPDTCVREFQRWQKATGASTFLLRLRHAHSGGPPHEKIMEAIRLFGERVLPY
                     A"
     gene            2360..2854
                     /locus_tag="VNU03_13665"
     CDS             2360..2854
                     /locus_tag="VNU03_13665"
                     /inference="COORDINATES: protein motif:HMM:NF037735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HWP75563.1"
                     /translation="MNAEDATRRVVFLDIDGVLAPIRRWDRYGELEPACIEVLNEIVA
                     SARADVVISSTWRHGRTVAELQAMLEAKGFTGRVVDKTPIGAPGAERGDEIAAWLAEH
                     AMGGYVIIDDHVDMGELRTELVLTHPGRGLQPADAPRAIEILMRADGRPRRVAGSSDS
                     GTPR"
BASE COUNT          426 a          998 c         1107 g          431 t
ORIGIN      
        1 cccctgatcg tgggaacgaa cctcgaggag atgaagctct accgcttcct cgacccgctg
       61 ctggagcggc tggacgaggc ggctctgctc cagcgctgtg gcctgctatt cccgggcacg
      121 ggccccgacg gtcgtaccca cgcggagcgc gcggtggata cctatcgctc ggcccggcgg
      181 gcacggggcg aggacgcctc cgcggtcgag acctggctcg ccatctccac cgaccacgtc
      241 ttccgggccg gcgccctcaa gctcgccgag ctccatgctc gtcacacgcc cgaggtcttc
      301 gtgtaccagt tcgagtggaa agggaaggag cccggccggc cgcagggggc catccacgct
      361 ctcgagctgc ccttcgtgtt cggtacgctg gcgacgtcgg agatcggggc gatcgcgggc
      421 cgcaccccgg cggcgcagac cctctccggg cagatgcagg acgcttggat cgccttcgtg
      481 cggggcgggc ggccccgcgc ggccgggctt ccggagtggc cgccctacgc cgcgccacgc
      541 cgggccacga tggtcctcgc ggatccgtcc gggctcgtcg atgcgcccgg ggagccggag
      601 cgggcggtgt gggacgcgtt cctgggctga cgattcaggg gttctaggtc tcgcgctccc
      661 gcgaggtctc gccggtcgcg agcggccaaa catagcgatc ccacgtgaga ccgaccacgt
      721 ccatctggcc ggtggcccgg gcgcccgacc gctcggcgac gcgccgcgag cgggcgttca
      781 gcgggagaat ggccgagatc gcgtacggca gtcgcaggac gtcgcgcgcg tagtcgcgaa
      841 cgcagcgcgc cgcctcggtc gcgaacccct ggccccagac ggcggggtcg agcgtgtagc
      901 caagctcgac ctcgaatgtc agcgcgacat cggcgggcgc ttcttcgcgc ccgaaccatc
      961 ctctgcgaat cccgtggtcg ggggccgcgg attcgacgac gaggtccatc aggccgcagc
     1021 gcccgatcaa ggctccgtct gccttgcgca cgaccgcgag atagccgagg gcgagctgct
     1081 cgtagctcgc gagggtgtac cgcagccagc ccgccgtctc ctcgggcgag cgcggccggc
     1141 cggccggata gagatgctgg gcgacctcgg ggagcgcgtg gatgcgagtg aaaaacggca
     1201 gatcgtccgg cgcgacccgg cgcagcacga ggcgggccga ctccagccgg tcggtgccgt
     1261 gcgccagggt catgtcgaag cctccttcag gcgtagggca ggacgcgctc gccgaagagc
     1321 cggatcgcct ccatgatctt ctcgtgcggc ggcccgcccg agtgggcgtg gcgtagccgg
     1381 agcaagaacg tgctcgcgcc ggtcgccttc tgccagcgct ggaactcccg gacgcacgtg
     1441 tcggggtccc ccaggatcag gcgatcgggg gcgagactgt ccagggtgaa ctccgagccg
     1501 gcggagagat tgcggaactc ggccaggcga ttctcccagt agtagcggta ggcggccatc
     1561 acgtgcggcc cgtagaccgc gtcggcctcg gcccgcgacc gggcgaccca ggcgtcgcgc
     1621 atctggacga cctcggccgg gcggccggcc tcgcgcgccg cctccttgta gacgtcggcg
     1681 agatgcgtgg cgttcgcgag gccggtgctg ggcgtgccga cgaagccatc ggcgatccgg
     1741 ccggcccggc gggcggcggc gtcgatgctg gcgccgatcc acagcggcgg ccccgacttc
     1801 tggtaggggc gcggccggat ctggacgtcc tcgagcgtgt agtgcttgcc gtggaacgag
     1861 aaccgctcgc ccgcccagca ccggcgcagg atctcgatgc tttcctcgaa gcgcccggcc
     1921 cggtcctcca tggggacgga gaaggcgcgg aagtcggcgg gctgatagcc gatgcccacc
     1981 cccaggatga cgcgcccctt ggagacgagg tcgagcgtga tcacgtcctc ggccacgtgc
     2041 accgggtgat ggagcggcag gaggatcacc gaggtgccga cgcgtagccg cgtcgtgcgt
     2101 gcggccaccg cggtggccac gatcaggggc gaggggagga agccgtcgtg atcctggtgg
     2161 tgctcgccga agaagcagga gtcgaagccg ctggcctcgg ccagctgggc ctcggcgatg
     2221 acctcgtcga tgcagcgatc gagctgctcg ccccgcggcg gattggcgat cgagctgtag
     2281 aggccgaatt tcatgcccca cctcctcggg gggattatgt cacagggatc ggggacacgc
     2341 gctggggtag acttcgaggg tgaacgcgga agacgcgacg cgacgggtgg tttttctcga
     2401 catcgacggg gtgctcgccc cgatccgccg ctgggatcgt tacggcgagc tcgagcccgc
     2461 atgcatcgag gtgttgaacg agatcgtcgc gagcgctcgc gcggacgtcg tgatctcgtc
     2521 cacctggcgg catggccgga cggtcgccga gctgcaggcg atgctggagg cgaaggggtt
     2581 caccggccgc gtggtcgaca agacgcctat cggggctccc ggcgccgagc ggggagacga
     2641 gatcgcggcc tggctcgcgg agcatgccat gggcggctac gtgatcatcg atgaccacgt
     2701 cgacatggga gagctgcgca ccgagctcgt cttgacgcac ccgggccgtg ggctgcaacc
     2761 cgccgacgcg ccgcgggcca tcgagatcct aatgcgagcc gacggccggc cgcgaagggt
     2821 tgccgggtcg tcggactccg gtacgcctcg gtagagaagc tggcgcccgt gcgtcggtgg
     2881 ctcttcaccg gctggaccgt ggtggcgggc gccttcttcg gctacgcgat cgcgcagggg
     2941 ctgatgcact cctacgcggt gt
//
LOCUS       DATMCV010000592         4331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_498542, whole genome shotgun sequence.
ACCESSION   DATMCV010000592 DATMCV010000000
VERSION     DATMCV010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498542"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VNU03_13670"
     CDS             complement(<1..304)
                     /locus_tag="VNU03_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP75564.1"
                     /translation="MEVLLDVRGLRTHFHTSTGVIRAVDGVSWDVRKGETVALVGESG
                     CGKSVSALSVMRLVSAPAGRIVGGEILFKGRNLLALSEEEMRRVRGREIGMIFQEPM"
     gene            complement(355..1266)
                     /locus_tag="VNU03_13675"
     CDS             complement(355..1266)
                     /locus_tag="VNU03_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWP75565.1"
                     /translation="MRWTRGDRSDDIEDRRGEGPTGGGGLRIGGGIGLGGAAILLVLS
                     LVFGQNFFALLDGSGDVSTAPGGGAPSAPPRKASPAEEQRADFISFVLDDVQNTWTRE
                     FQQMGRDYQRAHLVLFTGGTRSGCGVAETAAGPFYCPEDRKVYIDLAFYDELRSRFGA
                     PGDFAQAYVLAHEIGHHVQHLLGLTDQVRAQQERSPSRANALSVRLELQADCLAGIWA
                     HSTGERRLLEEGDVDEALGAASAVGDDRIQKQSGGRVSPESWTHGSARQRSGWFRRGL
                     ETGRLQDCDTFRTALPGVEERAPLRSR"
     gene            complement(1277..2407)
                     /locus_tag="VNU03_13680"
     CDS             complement(1277..2407)
                     /locus_tag="VNU03_13680"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HWP75566.1"
                     /translation="MDATPREPFLLTPGPLTTSRATKEAMLRDWGSRDGEFIAMNARV
                     RRRLLELVSGDGSHVCVPLQGSGTFIVEAMIGTLVPPDGKLLVLVNGAYGRRMVRMCE
                     YYRRACVVQETAEDQTVDPAALDATLARDPAVTHVVVVHCETTSGVLNPVEEVAAVTA
                     RRGRRLLIDAMSAFGAIPLDARRVSFDAVVASANKCLEGVPGIGFAIVRREALEEARG
                     QAPSLSLDLHDQWAAMEKNGQWRFTPPTHVIAALDRALDQHAAEGGVAGRGARYRRNC
                     TQLVEGLRALGLETLLPDRLQAPIIVTVRMPADPRFHFETFYERLNQRGYVIYPGKLT
                     VADSFRIGCIGALGEGEMRGVLDAIRIVLGELGVASGAPGRG"
     gene            complement(2435..>4331)
                     /locus_tag="VNU03_13685"
     CDS             complement(2435..>4331)
                     /locus_tag="VNU03_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP75567.1"
                     /translation="RGAVAAVETSDGPITTSILVNCAGMWARELGRMSGVTIPLHACE
                     HMYIVTQPIAGVSPDLPVLRDPDGHIYVKEEVRGLLMGGFDPWAKPWGTDRIPDPFSF
                     QTLKEDWEKFEILMTNAMQRLPDLEKAEVRTFLNGPESFTPDNYFILGEAPEVRRYYV
                     GAGFCSGGIAAAGGAGRALAEWIVEDRPPMDLWQADIRRFAPFHADRRFLRERVSEIV
                     GVHYNIAFPNRELTSGRDLRRSPLHERLRARRAAFGAKMGWERANWFAPEGVEPTPIY
                     SFGRQNWFPYAAAEHRAAREAVAVFDQTSFAKLRVQGADAEAALQRLCANDVAVPPGH
                     IVYTGMLNEQGGYESDLTVTRLAADAYLVVTGSAQSTRDLDWIRRHLPADARVKVTDV
                     TDAWAVLGVMGPRSRELLGRASGADLGSAAFPFGASREIAIGPATVRASRITYVGELG
                     WELYVPVAEAPGVYDVLQEHGRDLGLRDAGYYAMDSLRCEKAYRAWGREVTTEDTPWE
                     AGLGFAVRLDKAVPFIGREALVSRRTQPLTKRLLIFVLEDPDALPWGDEPILRDGRVV
                     GVVTSAAFGHTLGRAVAMGYVREPAGVDEAYIAGGAFALDVGGARVPARASLAAPYDP
                     RGLRIKG"
BASE COUNT          608 a         1607 c         1538 g          578 t
ORIGIN      
        1 tcatcggctc ctggaagatc atgccgatct cgcgcccgcg cacgcgacgc atctcctcct
       61 cggagagggc gagcagattg cgtcccttga agaggatctc gccgcccacg atgcggccgg
      121 cgggcgccga gaccaagcgc atgaccgaga gcgcgctgac cgacttgccg cagccggact
      181 cgccgacgag ggccaccgtc tcccccttgc gcacgtccca cgacacgcca tcgaccgcgc
      241 ggatcacgcc ggtcgacgta tggaagtgag tgcggagacc gcggacgtcg agcaggacct
      301 ccatgtggcg gggagtctaa cacaccgccg gagaggcccg ggcgaggcgg cgcctcagcg
      361 cgaccgcagc ggggcccgct cctccacgcc gggcagcgcg gtgcggaagg tgtcgcagtc
      421 ctggagccgc ccggtctcga ggccgcggcg gaaccacccg gaccgctgcc gggccgaccc
      481 gtgggtccag ctctccgggc tgacgcggcc gccggactgt ttctggatcc ggtcgtcgcc
      541 caccgcggag gccgcgccca gggcctcgtc gacgtcgccc tcctcgagga ggcggcgctc
      601 gccggtggaa tgcgcccaga tcccggccag gcagtcggcc tgcagctcga gccggaccga
      661 gagcgcgttg gcccgcgacg ggctgcgctc ctgctgggcg cgcacctggt cggtcaggcc
      721 caggagatgc tggacgtggt ggccgatctc gtgggccagc acgtacgcct gcgcgaagtc
      781 cccgggcgcg ccgaatcggc tccgcagctc gtcatagaac gcgaggtcga tgtagacctt
      841 gcggtcctcc gggcagtaga acgggccggc cgccgtctcg gcgaccccgc agccggagcg
      901 cgtgcctccg gtgaacagca ccaggtgggc ccgctgatag tcgcgcccca tctgctggaa
      961 ctcgcgcgtc caggtgttct gcacgtcgtc gagcacgaac gagatgaagt ccgcgcgctg
     1021 ctcctccgcc ggcgaggcct tgcgcggcgg ggccgacggc gcgccgccgc ccggcgcggt
     1081 ggagacgtcg ccgctgccgt ccagcagggc gaagaagttc tgcccgaaga ccaggctcag
     1141 caccagcagg atcgccgcgc cgcccaggcc gatgccgccg ccgatccgga gcccaccgcc
     1201 gccggtcggt ccctcgccgc ggcggtcctc gatatcgtcg ctgcggtccc ctcgcgtcca
     1261 ccgcatggcg gcctccctat ccccggccgg gcgcgcccga ggccacgccc agctcgccga
     1321 gcacgatgcg gatggcgtcc aggaccccgc gcatctcgcc ctctcccagg gccccgatgc
     1381 agccgatgcg gaagctgtcg gcgacggtga gcttgcccgg gtagatcacg tagccgcgct
     1441 ggttgagccg ctcgtagaac gtctcgaagt ggaacctggg atcggccggc atgcgcaccg
     1501 tgacgatgat cggcgcctgc agccggtccg gcaggagggt ctccaagccg agcgcgcgaa
     1561 gcccctccac cagctgcgtg cagttgcggc ggtagcgcgc tccgcgcccg gcgacgccgc
     1621 cctcggcggc gtgctggtcg agcgcccgat cgagcgcggc gatgacgtgg gtgggcgggg
     1681 tgaatcgcca ctgcccgttc ttctccatcg cggcccactg gtcgtgcagg tcgaggctga
     1741 gcgacggggc ctggccgcgc gcctcctcga gcgcctcgcg ccgcacgatc gcgaagccga
     1801 tgccgggcac gccctcgagg cacttgttgg ccgaggccac caccgcgtcg aaggacacgc
     1861 gccgcgcatc gagagggatg gcgccgaagg cgctcatcgc gtcgatcagg aggcgtcgac
     1921 cgcgccgcgc ggtcaccgcc gcgacctcct ccaccggatt cagcacgccg gaggtggtct
     1981 cgcagtgcac caccaccacg tgggtcaccg cgggatcccg cgcgagcgtg gcgtcgaggg
     2041 cggccgggtc gaccgtctgg tcctccgcgg tctcctgcac cacgcaggcg cgacggtagt
     2101 actcgcacat gcggaccatg cggcggccgt aggcgccgtt gacgagcacc aggagcttgc
     2161 cgtcgggcgg caccagggtg ccgatcatgg cctccacgat gaacgtcccg ctgccctgca
     2221 gcggcacgca cacgtggctg ccgtcgcccg agaccagctc gaggagccgc cggcgcaccc
     2281 gcgcgttcat ggcgatgaac tcgccgtccc gcgagcccca gtcgcggagc atcgcctcct
     2341 tcgtggcgcg actggtggtc agcggtccgg gggtgagcag aaacggctcg cgcggcgtcg
     2401 cgtccatggc ggtcgatcct ttccggttgc ccggctagcc cttgattcgc agcccgcgcg
     2461 ggtcgtacgg ggcggcgagg ctcgcccgcg ccgggacccg cgcgccaccg acgtcgaggg
     2521 cgaaggcgcc acccgcgatg taggcttcgt ccaccccggc cggctcccgc acgtagccca
     2581 tcgccaccgc ccgacccagg gtgtggccga aggcggccga ggtcactacg cccaccacgc
     2641 gcccgtcgcg caggatgggc tcgtcgcccc acggcagcgc gtccgggtcc tcgagcacga
     2701 agatcagcag ccgcttcgtg agcggctggg tccgccgact caccagcgcc tcccggccga
     2761 tgaacggcac cgccttgtcg agccggaccg cgaagcccag acccgcctcc cacggcgtat
     2821 cctcggtggt gacctcccgg ccccacgctc ggtaggcctt ctcgcaccgc agcgagtcca
     2881 tcgcgtagta cccggcgtcg cgcagcccga ggtcccggcc gtgctcctgg aggacatcgt
     2941 acacgcccgg ggcctccgcg accggcacat agagctccca gcccagctcg cccacgtagg
     3001 tgatgcgcga ggcccgcacg gtggccggtc cgatcgcgat ctcccgggag gcgccgaacg
     3061 ggaacgcggc acttccgagg tcggccccgc tcgcgcgccc gagcagctcg cgggagcgcg
     3121 gccccatcac cccgagcacc gcccaggcgt cggtcacgtc ggtgaccttg acccgcgcgt
     3181 cggcgggcag atggcggcgg atccagtcga ggtcgcgcgt gctctgggcc gatccggtga
     3241 cgaccaggta ggcatccgcg gccagacggg tgacggtgag gtcgctctcg tagccgccct
     3301 gctcgttcag catgccggta tagacgatgt ggccgggcgg gacggccacg tcgttcgcgc
     3361 agaggcgctg cagagccgcc tccgcgtccg ctccctgcac ccggagcttg gcgaacgacg
     3421 tctggtcgaa gacggccacc gcctcgcgcg cggcccggtg ctcggcggcc gcgtagggaa
     3481 accagttctg gcgcccgaac gagtagatcg gcgtgggctc gacaccctcg ggagcgaacc
     3541 agttggcgcg ctcccagccc atcttggctc cgaaggcggc gcgacgcgcg cgcaggcgct
     3601 cgtggagcgg ggagcggcgc aggtcccggc cgctcgtgag ctcgcggttc gggaacgcga
     3661 tgttgtagtg gacgcccacg atctcgctca cccgctcgcg gaggaagcgc cggtccgcgt
     3721 ggaagggagc gaagcgccgg atgtcggcct gccagagatc catgggcggc cggtcctcca
     3781 cgatccactc cgcgagcgcg cgccccgccc cacccgccgc cgcgatgccg cccgagcaga
     3841 agccggcgcc cacgtagtag cggcgcacct cgggggcctc gcccaggatg aagtagttgt
     3901 cgggggtgaa ggactcgggc ccgttgagga acgtgcgcac ctccgccttc tcgaggtcgg
     3961 gcagccgctg catcgcgttg gtcatcagga tctcgaactt ctcccagtcc tccttgaggg
     4021 tctggaagga gaagggatcg gggatgcggt cggtccccca cggcttggcc cacggatcga
     4081 agccgcccat caggagaccg cgcacctcct ccttgacgta gatgtggccg tcgggatcgc
     4141 gcaggaccgg caggtcgggc gagacgcccg cgatgggctg ggtcacgatg tacatgtgct
     4201 cgcaggcgtg tagtgggatc gtcaccccgc tcatgcggcc gagctcccgc gcccacatcc
     4261 ccgcgcagtt gaccaggatc gaggtggtga tggggccgtc ggaggtctcg accgcggcga
     4321 ccgctccccg c
//
LOCUS       DATMCV010000593         3996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_563696, whole genome shotgun sequence.
ACCESSION   DATMCV010000593 DATMCV010000000
VERSION     DATMCV010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563696"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1198
                     /locus_tag="VNU03_13690"
     CDS             293..1198
                     /locus_tag="VNU03_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWP75568.1"
                     /translation="MPSTARGQGIAALLIVVSAWGLTWPVNKVILLSLSPLWAVTLRS
                     AIATAALFALTAWRGRVALPPAGDLPVLISITLLHMVGFNLLASWGLGLVPTGRTVVL
                     AYTTPLWVTPGAALFLREPLTARRVVGVLVGLAGLVTLFNPLTFDWSDRAVVLGNLAI
                     LAGALLWAASILHIRAHRWRSTPFALIPWETLLATVLLAPIALAAGPPTLLEWTPEFT
                     LLLLYLGIVGTAVAYWAVATASRQLPAVTTSLGLLATPVVSAITATLWLGEPLTLQLV
                     VAIVLVLGGVAIGATDRAGAGPGAT"
     gene            1230..1757
                     /locus_tag="VNU03_13695"
     CDS             1230..1757
                     /locus_tag="VNU03_13695"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP75569.1"
                     /translation="MADYHATADTPAPSKAELLRALRSSRDEVVALISSLPPARLEEG
                     RYESGWNGRQILAHIASIEWSYPRLIDIARTPPSEPSAPPTRTMKGGNDSYNDRQVAK
                     REHLTVGELLAEFEKNRAATIQAVEAADEALFARRIRSAGGVVGPLALVFHQIAVAHV
                     LGHARDISGDTTHHG"
     gene            1750..2946
                     /locus_tag="VNU03_13700"
     CDS             1750..2946
                     /locus_tag="VNU03_13700"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP75570.1"
                     /translation="MAETNARGPLDGVKVLDMTEHMAGPFCTMILADMGADVIKLERP
                     GAGDSSRGMGDGTERNPYFRYINRNKKSLTLDYKGARGREIFLRLLPSMDVLVENYRA
                     TVMERAGLGWDVLRQANPRLIYAQLSGFGSDGPYREKGGFDLIAQGMGGIMHVTGEPD
                     GPPTSVGLPICDLGTGMWGAQGVLAALCERHRTGQGQKIECSLLETAVAFSSWTSAGW
                     LVDHEEPVRMGSRHRQNAPYQRFETKDGYMMIGAAGQSIWERCARALGHPKWLADPRF
                     AKATERRKNRFALEKEIVAVLTTAPTAHWTKVLDDAGVPCGPVYNYQQLFSDPQVKHR
                     EMVVHADDAELGRVPHIRTPIRMSRSAVAVRRTAPRLGQHTDEILAGLGYAPAQVAEL
                     RRERVV"
     gene            2984..3643
                     /locus_tag="VNU03_13705"
     CDS             2984..3643
                     /locus_tag="VNU03_13705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWP75571.1"
                     /translation="MAIFLIRHGETLGNASRTVQLPDNPLSPQGLAQAERLAKRLERE
                     GITAILASDYARAAQTAEQLARVTGVAVRRDPLLQERNFGDIRGTAYADLGFDMFEPD
                     YAPPNGETWEVFHARVDRAWALVQAAAAATPGHLAVVTHGLVCRSLAARHLTLPDGSE
                     VPERWENTAVTILDKLAAGADQTAAGADQSAATWRVRLLNCIAHLDDLSARPPAETGA
                     A"
     gene            3669..>3996
                     /locus_tag="VNU03_13710"
     CDS             3669..>3996
                     /locus_tag="VNU03_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75572.1"
                     /translation="MTRFTRPSLTRYLTWYLAGALLLTAALLWPAGGGAQQKPLRPYT
                     LILDFVPTGEYVPHYTALEKGYYREEGLDVKILRGQGSGDTVKRIAAGQGEVGIADIS
                     ALIAARA"
BASE COUNT          543 a         1533 c         1355 g          565 t
ORIGIN      
        1 gcgggcagcg gcacacagac cggcccccgg cagagcccct ccggcttggc gacccagccc
       61 gtcgcggcct ccgcgtcgcg cgccggacac cagagcgtcg tcgtcgaggc ggccgccccg
      121 tccaccgcga attcccccgc atccgtgagc agcgtgacca tggcgtgtcc tctctgggcg
      181 cgatcttacc atcgccacgc ggagcgggtc cgccttgacg cggcggtcgg cgcgcggcag
      241 tatcgcgccc gatgccccac gccctcgccg ccccgccgcc tcgcggctga tcgtgccttc
      301 gaccgctcgt ggccagggca tcgcggccct gctcatcgtc gtctccgcct ggggactcac
      361 gtggccggtg aacaaggtga ttctcctgag cctgtcgccg ctctgggcgg tgaccctgcg
      421 ctcggccatc gccaccgcgg ccctcttcgc cctcaccgcc tggcgcggcc gcgtggcgct
      481 gcccccggcg ggcgatctgc cggtgctgat cagcatcacc ctgctccaca tggtgggctt
      541 caacctcctc gcgagctggg gcctgggcct cgtgccgacc gggcgcaccg tggtgctggc
      601 ctacaccacg ccgctctggg tcacgcccgg cgcggccctc ttcctgcgcg agccgctcac
      661 cgcgcgccgg gtggtcggcg tgctggtcgg tctcgccggc ctcgtgacgc tcttcaaccc
      721 gctcaccttc gactggagcg atcgcgcggt cgtcctgggc aacctggcca tcctggccgg
      781 cgccctgctc tgggccgcca gcatcctgca catccgcgcg caccgctggc gctccacccc
      841 gttcgcgctg atcccgtggg agaccctgct cgccaccgtg ctgctggcgc cgatcgcgct
      901 cgcggccggc ccgccgaccc ttctggagtg gacgcccgag ttcaccctgc tgctgctcta
      961 cctgggcatc gtgggcaccg cggtcgccta ctgggcggtg gcgaccgcga gccggcagct
     1021 gcccgcggtc acgacctcgc tcgggctgct ggccacgccg gtggtgagcg cgatcaccgc
     1081 gacgctctgg ctcggcgagc cgctgaccct gcagctggtg gtcgcgatcg tcctggtgct
     1141 gggcggcgtc gcgatcggcg ccaccgaccg cgcgggcgcc ggtccggggg cgacttgaca
     1201 gcttctgcgc gtggccccat tctcccgcca tggccgacta ccacgcgacg gccgacactc
     1261 ccgctccgtc gaaggcggag ctattgcggg cgctgcgatc gagccgcgac gaggtcgtcg
     1321 cgctgatctc ctcgctgccg ccggcccgcc tcgaggaggg ccgctacgag agcggctgga
     1381 acggccggca gatcctggcc cacatcgcgt cgatcgagtg gagctatccg cgcctgatcg
     1441 acatcgcccg cacgccgccg tcggaaccgt ccgccccgcc cacccggacc atgaagggcg
     1501 gcaacgattc ctacaacgac cgccaggtgg ccaagcgcga gcacctgacg gtgggggagc
     1561 tgctggccga gttcgagaag aaccgcgccg cgaccatcca ggcggtggag gcggccgacg
     1621 aggcgctctt cgcccggcgc atccgctccg cgggcggcgt ggtcggcccg ctggccctcg
     1681 tcttccacca gatcgcggtg gcccacgtcc tcggccatgc gcgagatatc tccggagaca
     1741 cgacccacca tggctgagac gaacgcgcgc ggccccctcg acggcgtgaa ggtgctcgac
     1801 atgaccgagc acatggcggg ccccttctgc accatgatcc tggccgacat gggcgcggac
     1861 gtgatcaagc tcgagcgccc cggcgcgggc gactcttctc gaggcatggg tgatggtacc
     1921 gagcgcaatc cctatttccg ctacatcaac cgcaacaaga agagcctcac cctcgactac
     1981 aagggcgcgc gggggcgcga gatcttcctc cggctgctcc cgtcgatgga cgtgctggtg
     2041 gagaactatc gcgcaaccgt gatggagcgc gccgggctcg gctgggacgt cctgcggcag
     2101 gcgaaccccc gcctgatcta cgcccagctc tccggcttcg gctccgacgg cccctatcgc
     2161 gagaagggtg gcttcgacct gatcgcccag ggcatgggcg gcatcatgca cgtgaccggt
     2221 gagcccgacg ggccgcccac ctcggtgggc ctgccgatct gcgacctcgg caccggcatg
     2281 tggggggccc agggcgtcct ggccgcgctc tgcgagcgcc accgcaccgg ccaggggcag
     2341 aagatcgagt gctcgctgct cgagaccgcg gtggccttct cctcgtggac cagcgcgggc
     2401 tggctggtcg accacgagga gccggtgcgc atgggctccc gccaccgtca gaacgcgccc
     2461 taccagcgct tcgagaccaa ggatggctac atgatgatcg gcgcggccgg gcagtcgatc
     2521 tgggagcgct gcgcccgcgc gctcggccac ccgaagtggc tcgcggaccc gcgcttcgcc
     2581 aaggcgacgg agcggcgcaa gaaccgcttc gcgctcgaga aggagatcgt cgcggtgctc
     2641 accaccgcac cgaccgcgca ctggaccaag gtgctcgacg acgcgggggt tccgtgcggg
     2701 ccggtgtaca actaccagca gctcttctcc gacccgcagg tgaagcaccg cgagatggtg
     2761 gtccacgccg acgacgcgga gctgggccgc gtgccccaca tccggacccc gatccgcatg
     2821 tcgcggagcg cggtcgcggt gcggcggacc gcgccccggc tcggccagca caccgacgag
     2881 atcctggccg ggctcgggta cgcgccggcc caggtcgcgg agctgcgtcg cgagcgcgtg
     2941 gtctgagcgc gaccggcgcg gccgcccgcg accgcgagag cccatggcca tcttcctgat
     3001 ccgtcacggc gagacgctcg gcaacgcgtc gcgcacggtc cagttgcccg acaacccgct
     3061 gtcgccacag gggctcgccc aggccgagcg gctggccaag cggctcgagc gggagggaat
     3121 caccgcgatc ctggccagcg actacgcccg ggcggcccag accgcggagc agctcgcgcg
     3181 cgtcaccgga gtcgcggtcc gacgcgaccc gctgctgcag gagcgcaatt tcggcgacat
     3241 ccgcgggacg gcctatgccg atctgggctt cgacatgttc gagcccgact acgcgccgcc
     3301 caacggagaa acctgggagg tcttccacgc ccgcgtggac cgcgcctggg ccctcgtgca
     3361 ggcggccgcc gccgcgactc ccggccacct ggccgtggtc acccacggtc tcgtctgccg
     3421 ctcgctcgcg gcccggcacc tgaccttgcc cgacggctcg gaggtcccgg agcgatggga
     3481 gaacaccgcg gtcaccatcc ttgataagtt ggccgcgggc gctgatcaga cggccgcggg
     3541 cgccgatcag tcggccgcca catggcgcgt ccggctcctc aactgcatcg cgcacctcga
     3601 cgacctctcc gcgcggcctc cggccgagac cggcgcggct tgacccgcca ccaccacgga
     3661 gaatgccgat gacccggttc actcgcccgt cgctcacccg gtacctgacc tggtacctgg
     3721 ccggggccct gctcctcacc gcggcgctgc tctggcccgc gggcggcggc gcccagcaga
     3781 agccgctgcg cccgtacacg ttgatcctcg atttcgtccc gaccggtgag tacgtcccgc
     3841 actacaccgc cctggaaaag ggctactacc gagaagaagg cctcgacgtg aagattctcc
     3901 ggggccaggg gtcgggcgac acggtcaagc gcatcgcagc cggccagggc gaggtcggca
     3961 tcgcggacat ctcggccctc atcgcggccc gcgcca
//
LOCUS       DATMCV010000594         3851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_498816, whole genome shotgun sequence.
ACCESSION   DATMCV010000594 DATMCV010000000
VERSION     DATMCV010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498816"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VNU03_13715"
     CDS             complement(<1..336)
                     /locus_tag="VNU03_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP75573.1"
                     /translation="MDRRDDRRQFLKTGLAAGTMVGAAGLEGILAARRAPAVAQTTKI
                     HLLQWVDFIPEGDVEVRRQVAEYTKQTKVEVTFETINANDLQARITAAIQSGAGPDLI
                     LMLHNWPHLY"
     gene            598..1527
                     /locus_tag="VNU03_13720"
     CDS             598..1527
                     /locus_tag="VNU03_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWP75574.1"
                     /translation="MNKIERVRAALAGRPVDRAPFTVWYHFGNQHAPAERTAQVHLEF
                     FDHYDLDLLKLMNDYDYPMPAGLEVVATAQDLGRLQPFDPTKTPFGTQLQTVELVARA
                     LRDRALFVDTVFNAWNTLKRNLVREAMPGLMAEQPQAVEAALRIVNDNLIRYAQASLE
                     RGAAGIFLSVPATAESVTAEQYERFMRPFDLAFLEAIRGRGECHVLHAHGERLYFDRL
                     VDYPVHALSWADLNGGPSIAEARRRTPLTLMTGLDHARFVESSARRAREGVRAALAAG
                     GDTRFILAPGCSLPTYAYPPLIRAAREESAKSR"
     gene            1683..1904
                     /locus_tag="VNU03_13725"
     CDS             1683..1904
                     /locus_tag="VNU03_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75575.1"
                     /translation="MTPRRMALAAVLVLTVVFLSPIAYLAGQRAGVWLSAPAAERSGP
                     WPSVDEGERESAAPAERVKPQVRPGFGEI"
     gene            complement(1940..2680)
                     /locus_tag="VNU03_13730"
     CDS             complement(1940..2680)
                     /locus_tag="VNU03_13730"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP75576.1"
                     /translation="MSLTVRFVGSGDSFGSGGRFQTCIVVDGPRSRFAIDFGASSLIA
                     LAQQGIEHNSLDAILLTHLHGDHCGGVPFLLMDAMLAARRTRPLVIAGPRDLRPRLDA
                     LREALFPGSGVMAPKFPLEWIAMEPGRPQPVLDLVVTPEHARHTRETNPTALRVQVGD
                     RVVTYTGDTEWTDDVARAARDVDLLIAECYFHDKPVKWHLNYPDITARAGRVGAKRLI
                     LTHMSREMLAHAASVPEECAYDGLVVTL"
     gene            2743..3588
                     /locus_tag="VNU03_13735"
     CDS             2743..3588
                     /locus_tag="VNU03_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWP75577.1"
                     /translation="MRAFDRTFGAEFLAGVPAAPGVYRFHDAAGGLLYVGKAANLRRR
                     LAQYRLAGRRKKERKRRALVRAAVRITWEVCESPLAAALAEIRLIQALRPPRNVASAY
                     SFLYPFVGLHVEGDETYFCLTTSPEAFPAVAFHGAFRSREVTREAFYCLVELLRYVGH
                     PVPRHRCRRLGSARHSVVRGFRRLPAGSAAAWAGLLRGESRAALEALALRLVDHAGAR
                     ARRGEIHEALRSIARFFEEEARALARVRAATGFAAYPVPQLARDLLFTQIRLQPLETK
                     PAAAD"
BASE COUNT          560 a         1397 c         1325 g          569 t
ORIGIN      
        1 gtagaggtgc ggccagttgt ggagcatcag gatgaggtcc ggccccgccc ccgactggat
       61 cgcggcggtg atgcgcgcct gcaggtcgtt ggcgttgatg gtctcgaacg tgacctcgac
      121 tttcgtctgc ttcgtgtact cggcgacctg gcgtcgcacc tcgacgtcac cctccggaat
      181 gaagtctacc cattgcagca gatggatctt ggtggtctgc gcgacggcgg gcgcgcggcg
      241 cgccgcgaga ataccttcga ggccggcggc cccgaccatc gtgcccgccg cgagcccggt
      301 cttgaggaac tgccgtcgat cgtcccgtcg gtccatacgc acctccttgt gagtgaggac
      361 aggtcaggca gaagtgcagc tcgatccgaa gatgcgaggc gggagcgcta cggggggcgc
      421 aggtgggcgg acgacgggcg ccggccatcc gagtggtcgg aacgggcgga ggagacacat
      481 cccgtgaccc gatcccgatg catgactccc tcgtcagcct acggggctcg aaagtgcggt
      541 gactctgcct ctggacgcgg ccgctgtcaa gggagacccc gtgctataac ttccgccatg
      601 aacaagatcg agcgtgtgcg ggcggccctg gccggccggc ccgtggaccg ggcccccttc
      661 acggtgtggt atcacttcgg caaccagcac gctcccgccg agcgcaccgc gcaggtgcac
      721 ctcgagttct tcgaccacta cgatctcgac ctgctcaagc tcatgaacga ctacgactac
      781 ccgatgccgg ccggcctcga ggtcgtcgcc accgcccagg acctgggccg gctccagccg
      841 ttcgatccga ccaagacccc gttcggcacc cagctccaga cggtggagct ggtcgcgcgc
      901 gccctccgcg accgggcgct cttcgtggac acggtgttca acgcctggaa caccctgaag
      961 cgaaacctgg tgcgcgaggc gatgcccggc ctgatggccg agcagccgca ggccgtcgag
     1021 gccgccctcc ggatcgtcaa cgacaacctg atccgctacg cccaggccag cctggagcgc
     1081 ggcgcggccg gcatcttcct ctcggtgccg gccaccgcgg agagcgtgac cgccgagcag
     1141 tacgagcgct tcatgcggcc cttcgacctg gccttcctcg aggccatccg cgggcgtggc
     1201 gagtgccacg tgctccacgc ccacggcgag cggctctact tcgatcgcct cgtggactat
     1261 ccggtccacg cgctttcctg ggccgacctc aacggcgggc cctcgatcgc ggaagcccgc
     1321 cggcgcaccc cgctcacgct gatgaccggc ctcgaccacg cgcggttcgt cgagagctcc
     1381 gcccgccggg cccgggaggg cgtccgcgcc gctctggcgg ccggcggtga cacccgcttc
     1441 atcctggccc ccggctgctc gctgccgacc tacgcctacc ctcccctgat ccgcgcagcc
     1501 cgggaggagt cggcaaagag ccggtaggcc acgaggaagc tgcccggcag gcctgggaga
     1561 aaccctgccg ataaggggta aatccctgcc tctatcctgc attgcatagt cgtaactggc
     1621 tggaaaacat gcaggattct cctggaatgg cgcttgctct gagagccccc aggagggcgt
     1681 cgatgacccc gagacgaatg gccctggcag ccgtgctggt cctgaccgtg gtgttcctga
     1741 gcccgatcgc ctacctcgcg ggacagcgag cgggagtctg gctcagcgcg cccgctgccg
     1801 agcgaagcgg tccgtggccg agcgtggacg agggcgagcg cgagtccgcg gcgcccgccg
     1861 agcgcgtcaa gccccaggtg cgtcccggct tcggcgagat ctagccccgc cgagcgagcc
     1921 ccaccccgct ccgcgagatc tagagcgtca ccaccagccc gtcgtacgcg cactcctccg
     1981 gcaccgaggc cgcgtgcgcg agcatctccc ggctcatgtg agtcaggatc aggcgcttcg
     2041 ccccgacccg gccggcccgc gccgtgatat cggggtagtt caggtgccac ttcaccggct
     2101 tgtcgtggaa gtagcattcc gcgatcagta gatccacgtc ccgcgcggcg cgcgccacgt
     2161 cgtcggtcca ctcggtgtcg ccggtgtagg tcaccacccg gtcgcccacc tgcacgcgga
     2221 gcgcggtggg attggtctcg cgggtgtgcc gggcgtgctc cggggtcacc acgaggtcga
     2281 gcaccggctg cggccggccg ggctccatcg cgatccactc gagcgggaac ttcggcgcca
     2341 tcacacccga gccggggaag agcgcctcgc gcagggcgtc caggcgcggg cgcaggtcgc
     2401 gcgggcccgc gatgacgagc gggcgggtgc gccgggccgc gagcatcgcg tccattaaca
     2461 gaaatggcac gccgccgcag tggtcgccgt gcaggtgggt gagcagaatc gcgtcgagcg
     2521 agttgtgctc gatcccctgc tgggcgagcg cgatcagcga cgaggcgccg aagtcgatgg
     2581 cgaagcggga gcgcgggccg tccaccacga tgcaggtctg gaagcgcccg ccgctgccga
     2641 acgaatcgcc cgagcccacg aagcgaaccg tcagggacat ggcgacctga tggtacccga
     2701 aatagctcgc ggggcggacc cggctcctga gaggatgccc acgtgcgcgc gttcgatcgg
     2761 acgttcggcg cggagttcct ggccggagtg ccggccgccc ccggggtcta ccgcttccac
     2821 gacgcggcgg gcgggctgct ctacgtcggg aaggccgcga acctccgacg gcgcctcgcg
     2881 caataccgcc tggctgggcg ccgcaagaag gagcgcaagc gccgggcgct ggtgcgcgcc
     2941 gccgtccgca tcacctggga ggtgtgcgag tccccgctcg ccgcggcgct cgcggagatc
     3001 cgactcatcc aggcgctgcg cccgccgcgg aacgtggcga gcgcgtactc gttcctctac
     3061 ccgttcgtcg ggctccacgt ggagggcgac gagacctact tctgcctgac cacctcgccg
     3121 gaggccttcc ccgcggtcgc gttccacggg gccttccgct cccgggaggt cacccgcgag
     3181 gcgttctact gcctggtgga gctgctccgc tacgtgggtc acccggtgcc gcgccaccgg
     3241 tgccggcggc tcggctcggc ccgccactcg gtggtccgag gcttccgccg cctgcccgcg
     3301 ggcagcgcgg cggcgtgggc cgggctcctg cgcggcgagt cgcgtgcggc gctcgaggcg
     3361 ctcgcgctcc gcctagtcga ccacgcgggc gcacgggccc ggcgtggtga gatccacgag
     3421 gcgctccggt ccatcgcgcg cttcttcgag gaggaggccc gcgccctggc ccgggtgcgg
     3481 gcagccaccg ggttcgccgc ctatccggtg ccgcagctgg ctcgcgacct cctgttcacc
     3541 cagatccggc tgcagccgct cgagaccaag cccgccgcgg ccgactgacg tgccttgtca
     3601 gagatcggac tcgcccgcgt aacacggtaa gatcgtcatc accgtaaagt cccgtcgtca
     3661 cgagctaaat aggctggcac tcgcggtgca tacctactgg gcatgtcaga agcgcgcgcg
     3721 gcccgccgta tcctcgtcgt cgacgacgac gacgccctcc gagaggtgct caccgtcgcc
     3781 ctgacccgtg acgggcacac cgtcgacgga gcctgcgacg gcaccgaggc cctggccctg
     3841 ctggaccgcc a
//
LOCUS       DATMCV010000595         6800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_631495, whole genome shotgun sequence.
ACCESSION   DATMCV010000595 DATMCV010000000
VERSION     DATMCV010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631495"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VNU03_13740"
     CDS             <1..1064
                     /locus_tag="VNU03_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75578.1"
                     /translation="GIEPAALTHPAWGLSDRVWQLEPDAPWAPREALTCFQAVDYARP
                     DFIPPLAEPRRLPGGGGTALLNLIAGLMKDHGVATVRYRGPYPTEQLFTALLECFRYD
                     AAEALPLERFLADGGLDWHPAPFETHHVTPGVVVQLRHEVDKVMLDGVSFYRVEWQDI
                     RRREPRVIRTDGDRVICSLWALNGPIEDRLVLDRLGEIRETPVSAVDASAPAPLPPVW
                     TVALGELIARESAPALAPDIARVTAGLSLEWGPVPGDLLRVQGQRVRLSRRLREAGTA
                     ALAGAASPPERAEQALRFVLEVARLLGPEVRLRAQARLETLDEAEQRRLLEDAPAELP
                     EALGESVGRLLALVIRGGG"
     gene            complement(1171..2424)
                     /locus_tag="VNU03_13745"
     CDS             complement(1171..2424)
                     /locus_tag="VNU03_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75579.1"
                     /translation="MSELGDKAVEARPKRTRIELLVRDEGGQGFETREIPSAVAYLHS
                     RPQRIPDADWEMARLPYAPQGVVTYALKNRRTEVIIQITETEKFLWGQMDGRASLQEI
                     ATSYLLHYGSFDFEIIPTLIVKLLRADLLTMRPVSRLRAVLARNRRNPAARAMEGTLH
                     ALERLTVTSRRSHQFFERMYRYGGFLLFTPWALAVLAIMVVVGGRSAITVWRDLGEVT
                     HGLASHPLVAILLVKIVFWLTVVNHQIIHALACIHYRRRVREFGFTILHGFIPTFYAD
                     VTDIFMATRRARIVNAVAGPLFHLFLGFFCFWIAARLEPGLIQGFLAASALLQLQSFV
                     ISLYPFWFLEMDGYHILVEMVGVPTLNHDSVRFVRESLWRRIRTATWLTRQEAICLAY
                     FTLSVASLAGFVLLNVWLFTRAGKS"
     gene            complement(2444..2944)
                     /locus_tag="VNU03_13750"
     CDS             complement(2444..2944)
                     /locus_tag="VNU03_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWP75580.1"
                     /translation="MASLPEIGFLRRVVLFKDVDEPVLLALCPSFRERRLKKGDVLFR
                     AADPGEELFLIRDGSIVVSKPVTGRVEQVLSRLGAGEAFGEMSLFGDERRRSATCQAE
                     LDSLLYSLDRDSLNRFVTGSPAAAAKFFQQMAQVAFKRLRDSSDLVAEVTRWGLEATG
                     LDIEHK"
     gene            complement(2945..3166)
                     /locus_tag="VNU03_13755"
     CDS             complement(2945..3166)
                     /locus_tag="VNU03_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75581.1"
                     /translation="MAGKGIQDLVGQVMIDKEFLADLVSDPARVLAGFELTADERAAI
                     MLALGSTSTVSDRERARALQNVMLKRWAT"
     gene            complement(3254..4498)
                     /locus_tag="VNU03_13760"
     CDS             complement(3254..4498)
                     /locus_tag="VNU03_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75582.1"
                     /translation="MGVWTSAWASARIAVRALRVNKLRSALTMLGIVIGVGAVITMVA
                     VGAGAQARVAEQIQSLGSNMIIVLSGSILSGGVRMGSGSQLTITEDDAWAIQREIPAV
                     AAAAPTSRGNAQVVYGNLNWATGIQGVTLEFFTAREWDVADGRLFSQEEVEGAGKVAL
                     VGLTVVGNLFGDSDPLGQVIRIKNVPFTVIGLLERKGQTTFGQDQDDTVLIPLSTAKK
                     KVLGASQANARSVGSIAVKVREARAMPEAEQEIRGLLRQRHRLQAFQDDDFNIRNLTE
                     VLQSQEASSRVLTLLLAAIASVSLLVGGIGIMNIMLVSVTERTREIGLRMAVGARGRD
                     ILLQFLVEAVTLSLIGGAIGIAMGLAGSYSIAYFAQWRTLVSTEAVFVAFAFAAAVGV
                     FFGFYPARKAAALNPIDALRYE"
     gene            4710..5222
                     /locus_tag="VNU03_13765"
     CDS             4710..5222
                     /locus_tag="VNU03_13765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP75583.1"
                     /translation="MEPSHSHDTIVTAHYGRLRRLCRLMLGDPQEAEDVVQDVFLKAH
                     EAERLGRPPMDWGAWLTRVAVNACRDRRRAGGWMRFRLRSTHIDDLPLVADTLAPSEA
                     AVSEDTRRRIWVAFRALPRRQQEVFALRYIEEQPTTDVALALGLSAGSVKRHLYRAVR
                     HLRRALGDAR"
     gene            5219..5686
                     /locus_tag="VNU03_13770"
     CDS             5219..5686
                     /locus_tag="VNU03_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75584.1"
                     /translation="MSPCLSDDALDRVHADLGSIAERAHLAGCAACTGRSRQLRRDLD
                     LITAVLRDTREPLTQPLTQIAPARRRWLPAAIGLSTAALALLVWVEVAVWRAVTPLPP
                     TMQPQEVAAILSDVSASLFSVNGDPVPATGDDGPLSLEGMAREVDSTLTGGEQ"
     gene            5683..6168
                     /locus_tag="VNU03_13775"
     CDS             5683..6168
                     /locus_tag="VNU03_13775"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HWP75585.1"
                     /translation="MIRMRSLAGVLVGVLLLAAAGPALAQAPGRESSMRGGGRGDGEL
                     WLMIRASNLTPEQQTRVRAILSAHRKSASPVVEQLRQAQQELGTKLLAPGQVQAADLQ
                     PQLQRISQLRDQLAQDSAAAALEVRAVLTPEQLARVAQTKDRLKQLREEMRQLMQPAR
                     P"
     gene            6175..6462
                     /locus_tag="VNU03_13780"
     CDS             6175..6462
                     /locus_tag="VNU03_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75586.1"
                     /translation="MRDLLALLVLAVGLALPVLAHASPPDPSWIPGVYDDADFDDVVT
                     RVVSSTGSVTPGALDALGFIPRRAECLGHAVDTAPDSLATDAGHCRAPPAS"
     gene            6584..>6800
                     /locus_tag="VNU03_13785"
     CDS             6584..>6800
                     /locus_tag="VNU03_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWP75587.1"
                     /translation="MLEQAFTCSEPLAAIRERDRDGNLWAVVLAGGEGSRLRALTRYL
                     YGDERPKQYAVLTGSKSLLRQTLERVAR"
BASE COUNT         1004 a         2494 c         2361 g          941 t
ORIGIN      
        1 tcggcatcga gcccgccgcc ctgacccacc ccgcgtgggg cctctccgac cgggtgtggc
       61 agctcgagcc cgacgcgccg tgggcgcccc gcgaggcgct cacctgcttc caggcggtcg
      121 actacgcgcg cccggacttc atcccgccgc tcgccgagcc gcgccgcctg cccggcggcg
      181 gcgggaccgc gctgctgaac ctgatcgcgg gcctcatgaa ggaccacggc gtcgccaccg
      241 tgcgataccg cgggccctac ccgaccgaac agctcttcac cgcgctgctc gagtgcttcc
      301 gctacgacgc ggccgaggcc ctgccgctcg agcgcttcct cgccgacggc gggctcgact
      361 ggcaccccgc cccgttcgag acgcaccacg tgacgcccgg tgtggtggtg cagctccgcc
      421 acgaggtgga caaggtgatg ctcgacgggg tctcgttcta ccgcgtcgag tggcaggaca
      481 tccgacgccg cgagccccgc gtcatccgta ccgatggtga ccgggtgatc tgctcgctct
      541 gggcgctcaa cgggccgatc gaagaccggc tcgtgctcga ccgcctgggc gagatccgcg
      601 agacgcccgt gtcggcggtc gacgcgagcg cgccggcgcc gcttcccccg gtgtggacgg
      661 tcgccctcgg cgagctgatc gcgcgcgaga gcgcgcccgc gctggccccc gacatcgcgc
      721 gggtcaccgc cgggctctcg ctcgagtggg gcccggtgcc gggcgacctg ctgcgcgtgc
      781 agggccagcg ggtgcgcctg agccggcgcc tgcgcgaggc cggcacggcc gccctggccg
      841 gcgccgcctc acccccggag cgtgccgagc aggccctgcg cttcgtgctg gaagtggccc
      901 gcctcctggg gcccgaggtg cgcctccggg cccaggcccg cctcgaaacc ctcgacgagg
      961 ccgagcagcg ccgcctcctg gaagacgcgc ccgccgagct acccgaagcg ctcggcgagt
     1021 ccgtcggccg cctgctggcg ctcgtgatcc ggggcggcgg atgaggggcg ggaccgaaga
     1081 agtaaacggt cgcccgccaa cctgggggag tgcgggggcc atctctgggc ccccgcagat
     1141 gaagagcctg aagaactaaa cggtcgcccg ctacgacttc ccggcccgcg tgaacagcca
     1201 cacgttcagc agcacgaagc ccgcgaggga ggcgaccgac agggtgaagt aggcgaggca
     1261 gatcgcctcc tggcgggtca gccaggtcgc ggtccggata cgccgccaga gggattcgcg
     1321 cacgaaccgg accgagtcgt gattgagcgt gggcacgccc accatctcca ccaggatgtg
     1381 gtagccgtcc atctcgagga accagaacgg gtacaggctg atcacgaagg actgcagctg
     1441 cagcagcgcc gaggcggcga ggaagccctg gatgagcccg ggctcgagcc gcgccgcgat
     1501 ccagaagcag aagaagccca ggaagaggtg gaagagcggc ccggccaccg cattcacgat
     1561 gcgggcgcgg cgggtggcca tgaagatgtc ggtgacgtcg gcgtagaagg tcgggatgaa
     1621 gccgtgcagg atggtgaagc cgaactcgcg cacccgccgc cggtagtgga tgcaggccag
     1681 cgcgtggatg atctggtggt tgaccaccgt gagccagaac acgatcttca ccaggaggat
     1741 cgccaccagc gggtggctcg cgagcccgtg ggtcacctcg ccgaggtcgc gccagacggt
     1801 gatggcgctg cggcctccca ccaccaccat gatcgccagc accgccaggg cccacggcgt
     1861 gaacagcagg aaccccccgt accggtacat ccgctcgaag aactggtgcg agcggcggct
     1921 cgtgacggtc aggcgctcga gcgcgtggag cgtgccctcc atcgcgcggg cggccgggtt
     1981 gcgccggttt cgcgccagca ccgcgcgcaa gcgcgacacc ggacgcatgg tgaggaggtc
     2041 ggcgcgcagg agcttgacga tcagggtcgg gatgatctcg aagtcgaagg agccgtagtg
     2101 tagcaggtag gacgtcgcga tctcctggag cgaggcgcgc ccgtccatct ggccccagag
     2161 gaacttctcg gtctcggtga tctggatgat cacctcggtg cggcggttct tgagcgcgta
     2221 ggtgaccacc ccctgcgggg cgtaaggcag gcgcgccatc tcccagtccg cgtcggggat
     2281 gcgctggggg cggctgtgga ggtaggcgac cgcgctcggg atctcgcgcg tctcgaagcc
     2341 ctggccgcct tcatcccgga ccagcagctc gatccgcgtg cgcttcggtc tcgcttccac
     2401 ggccttgtcg ccgagctcgc tcaccctctc gcgctccgga gcgctacttg tgctcgatgt
     2461 cgaggccggt ggcctccagc ccccagcgcg tcacctcggc gaccaggtcg gaggagtcgc
     2521 gcagccgctt gaaggccacc tgcgccatct gctggaagaa cttcgcggcc gcggccgggc
     2581 tgccggtgac gaagcgattg aggctgtcac ggtcgaggct gtagagcagg ctgtcgagct
     2641 cggcctgaca agtggcggac cggcgacgct cgtcgccgaa gaggctcatc tcgccgaagg
     2701 cctcccccgc gccgagccgc gacagcacct gctcgacccg cccggtcacc ggcttcgaga
     2761 ccacgatgct gccgtcccgg atcaggaaca gctcctcgcc cggatcggcc gcgcgaaaga
     2821 gcacgtcccc cttcttgagc cggcgctcgc ggaagctcgg acacagggcg aggaggacgg
     2881 gctcgtccac gtccttgaac agcacgacgc gtcgcaggaa accgatctca gggagactgg
     2941 ccactcaggt ggcccagcgc ttgagcatga cgttctgcag ggccctcgcc cgctcccgat
     3001 cggagacggt cgatgtgctg cccagggcga gcatgatcgc ggcgcgctcg tcggcggtca
     3061 gctcgaaccc cgccagcacc cgcgcggggt cgctcaccag atcggcaagg aactccttgt
     3121 cgatcatcac ctgcccgacc aggtcctgga tccccttgcc cgccacgcca cgacctcgac
     3181 ttttccgggc ccaagatccc tggtcccaga aggccaattt agcagaagtg gcgcggtttt
     3241 aaaagcctct tcctcactcg tagcggaggg cgtcgatggg gttgagggcc gccgccttgc
     3301 gggccgggta gaagccgaag aacaccccca ccgcggccgc gaaggcgaag gccacgaaga
     3361 ccgcctcggt gctcaccagg gtgcgccact gcgcgaagta ggcgatcgag taggagcccg
     3421 ccagccccat cgcgatgccg atggctccgc cgatcaggga gagggtcacc gcctccacca
     3481 ggaactggag caggatgtcc cggccgcggg ccccgaccgc catgcggagc ccgatctccc
     3541 gggtgcgctc ggtgaccgag accagcatga tgttcatgat gccgatgccc cccaccagca
     3601 gcgagacgga cgcaatggcg gcgagcagga gggtcagcac ccgggacgag gcctcctgcg
     3661 actgcagcac ctcggtcagg ttgcggatgt tgaagtcgtc gtcctggaag gcctgcagcc
     3721 ggtggcgctg acgcagcaac ccgcggatct cctgctcggc ctccggcatg gcgcgcgcct
     3781 cccgcacctt gaccgcgatc gatcccaccg accgcgcatt ggcctggctc gcgcccagca
     3841 ccttcttctt ggcggtgctg agggggatca gcaccgtgtc gtcctggtcc tggccgaacg
     3901 tggtctgccc cttgcgctcg aggaggccga tgaccgtgaa gggcacgttc ttgatccgga
     3961 tcacctggcc gagcgggtcg ctgtcgccga agaggttgcc caccacggtg aggccgacga
     4021 gcgccacctt gcccgccccc tcgacctcct cctggctgaa caggcgcccg tcggccacgt
     4081 cccactcccg cgcggtgaag aactcgagcg tgacgccctg gatgccggtg gcccagttga
     4141 ggttgccgta cacgacctgg gcgttgccgc gggaggtggg cgccgccgcg gccaccgccg
     4201 ggatctctcg ctgaatggcc caggcgtcgt cctcggtgat cgtgagctgg ctgcccgagc
     4261 ccatgcgcac gccgcccgac aggatcgagc ccgagaggac gatgatcatg ttcgagccaa
     4321 ggctctggat ctgctcggcg acgcgcgcct gggcgcccgc gcccaccgcc accatggtga
     4381 tgaccgcgcc aaccccgatc acgatgccca gcatggtgag cgcgctgcgg agcttgttca
     4441 cccggagcgc ccggaccgcg atccgggcgc tggcccacgc gctggtccag acgcccatgc
     4501 cgtcctcctc cgcccgcctg cggatccgct catcatctgc tgacgggccg gagcccgcgg
     4561 gagctcgacc gagacggctc ccaggtttct gacgagccag ccccttcttc agttgaccgc
     4621 caccgggcac tgaccccgcc cgtgtcaacg gaccgaaggt ttcgttcgtc aggaccgatg
     4681 agagggcacg ggtcaaccgt cagagagacg tggagccctc gcacagtcac gatacgatcg
     4741 ttaccgccca ctacgggcgg ctccggcggc tctgccgcct gatgctgggt gacccccagg
     4801 aggccgagga cgtggtgcag gacgtgttct tgaaggcgca cgaggcggag cggctcggcc
     4861 ggcccccgat ggactggggc gcctggctca cccgggtcgc ggtcaacgcc tgccgggacc
     4921 ggcggcgggc cggaggctgg atgcgcttcc gcctccggag cacccacatc gacgacctcc
     4981 cgctggtggc cgacaccctg gcccccagcg aggcggcggt cagcgaggac acgcggcggc
     5041 gcatctgggt ggccttccgc gccctgccgc gccgccagca ggaggtattc gctctgcgct
     5101 acatcgagga gcagccgacc accgacgtcg cgctcgcgct cgggctgagc gcgggcagcg
     5161 tcaagcgcca tctctatcgc gccgtgcgcc atctccggcg cgcgctcggg gacgcgcgat
     5221 gagcccctgc ctctccgacg acgccctcga ccgcgtgcac gccgacctcg gctcgatcgc
     5281 ggagcgcgca cacctggccg gctgtgccgc ctgcaccggc cgatcgcggc agctgcgccg
     5341 tgacctggac ctcatcaccg cggtgctgcg cgacacgcgc gagcccctga cccagcctct
     5401 gacccagatc gcccccgcgc gccgacgctg gctgcccgcc gcgatcgggc tctccaccgc
     5461 cgccctcgcc ctgctcgtct gggtggaggt ggcggtctgg cgcgcggtga ccccgctgcc
     5521 gcccacgatg cagccgcagg aggtggccgc catcctctcg gacgtctcgg cgtcgctctt
     5581 ctcggtgaac ggcgacccgg tccccgccac cggcgacgac ggaccgctga gtctcgaggg
     5641 catggcccgc gaggtcgaca gcacgctgac cggaggcgag caatgatccg gatgcgcagc
     5701 ctcgccggcg tgctcgtggg cgtgctgctg ctggccgcag ccgggcccgc gctcgcccag
     5761 gcccccggcc gcgagtcctc gatgcgaggg ggaggccgcg gcgacggcga gctctggttg
     5821 atgatccgcg cctccaacct cacccccgag cagcagaccc gcgtgcgcgc catcctgtcg
     5881 gcgcaccgga agagcgcgag cccggtcgtc gagcagctcc gccaggccca gcaggagctg
     5941 gggaccaagc tgctcgcccc ggggcaggtg caggccgccg atctccagcc gcagctccag
     6001 cggatcagtc agctgcgcga tcagctcgcc caggacagcg cggcggcggc tctcgaggtc
     6061 cgggccgtgc tcacccccga gcagctcgcg cgcgtcgcgc agacgaagga ccgtctcaaa
     6121 cagctgcgcg aggagatgcg gcagctgatg cagccggccc ggccctagtc ctcgatgcgc
     6181 gacctcctcg ccctcctcgt cctggccgtg gggctggccc tgccggtgct cgcccacgcg
     6241 agcccgcccg acccctcgtg gatcccgggc gtgtacgacg acgcggactt cgacgacgtc
     6301 gtgacgcgcg tggtgtcgag caccgggagc gtcacccccg gggccctcga tgcgctcggt
     6361 ttcatcccgc gccgcgcgga gtgcctcggt cacgcggtcg acaccgcgcc cgattcgctc
     6421 gcgacggacg cggggcactg tcgcgcccct cccgcctcgt agcgccttca tccccttcgt
     6481 cgccggccgg ctcgcgggct ctctctgagg gttcgtccgc ggggcctcca cgccccggcg
     6541 acgcgtcgaa acccgaacgg aggtgacaac gaggaggagg tccatgctcg agcaggcatt
     6601 cacgtgcagc gagccgctcg ccgcgatccg ggagcgagac cgagacggta acctgtgggc
     6661 cgtcgtgctg gccggggggg agggcagccg gctccgcgcg ctgacccgct atctctacgg
     6721 cgacgagcgg cccaagcaat acgcggtcct gaccggctcc aagtcgctcc tgcggcagac
     6781 cctggagcgc gtcgcgcggc
//
LOCUS       DATMCV010000596         3855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_899693, whole genome shotgun sequence.
ACCESSION   DATMCV010000596 DATMCV010000000
VERSION     DATMCV010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899693"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VNU03_13790"
     CDS             <1..779
                     /locus_tag="VNU03_13790"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75588.1"
                     /translation="PAPPTFCYWAPGPAAAEWCRMQNDAIAELVSRHPTRLLGAGGLP
                     LQAPDLAVKELERVVSVLRFPAVEIGANVAGLDLDDAALAPVWDAAAALDVAIFVHPQ
                     APILGDPRFRKDNLTQVAGFPLETALAMTRVIFGRVLDRWPTLRWCFAHGGGAFAYIL
                     PRLDQGWTAIADAHAAISQPPSAYARRVWVDSLTLSPRVLAFALETFGPERIVLGSDY
                     PFKMGVADPIAALDGVTLDAATRQRLLGDNAREFLALGPA"
     gene            811..1833
                     /locus_tag="VNU03_13795"
     CDS             811..1833
                     /locus_tag="VNU03_13795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75589.1"
                     /translation="MNFGTFLLMQSPSARTSREMYSRAIEMAQSAETLGFHNIWLAEH
                     HFSTYGYLSRPVQLATYIAARTTRIRVGTAVIVVPLHHPLVVAEEIATLAILSGDRLD
                     IGLGRGYQHYEFERFGLELESGRARWEESVDVILKALAGGPFSYEGKIYKIPETMIFP
                     QMEQPRPPIWITAQSPDSVEGAVRRGFNVLTGGFGVPVERMAEFRRLFDRVVAEVKPP
                     TPLAVGVQRAVYVTTSEADARAAAEEARWNMRVTLSLRNHYERVENGRAIAVPAPKEP
                     NVDDLLDRFLVIGTPETCVRQIQRLQRDVGITHFNCSFWFGDLEQARVLRSMELFARE
                     VMPAFA"
     gene            1847..3088
                     /locus_tag="VNU03_13800"
     CDS             1847..3088
                     /locus_tag="VNU03_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP75590.1"
                     /translation="MPTPGPRHEWPGASLARVPYWVYQDEDNYRAELRRIFEGPTWSY
                     ACLESDLEKPGDYRTTFLGEMPIIVVRGAAGEIHAFENRCAHRGALIALDDGGSAGSH
                     FQCVYHAWTYDLRGSLVGIAFEKGSNGRGGMPESFCKADHGPRKLRTATLHGLVFASL
                     SAETPPIEEYLGAEVLGRIRRVLRGPMRVMGRFTQALPNNWKLYVENVKDTYHASLLH
                     AFFGTFRITRLTQGGGILVSPDGGHHASYTIDRADDRTSTAYREQGIRSEKDRYRLVD
                     PSLLDSVDEWGDGIQLQILTVFPGFVLQQINNCLAVRQVVPKGVGGMELHWTYFGFAD
                     DSDDMRRRRLKQANLVGPAGYVSMEDGCVGGFVQRGVAAAGDEVSVVNMGGEGAESQE
                     TRATEASVRGFWKAYRRYMGE"
     gene            3090..3551
                     /locus_tag="VNU03_13805"
     CDS             3090..3551
                     /locus_tag="VNU03_13805"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HWP75591.1"
                     /translation="MLGERVAQLNAEYARAIDDDRLEQWPALFTDPCLYKITSADNYR
                     RGLEAGLIYADSRGMLQDRVAALREANIYERQAYRHLVGLPAIRPESNGALHAETPFL
                     VVRVMRDGTMDLFATGRYVDVMVDEGGTLRFRERVVVCDSSRVDTLLAIPL"
BASE COUNT          538 a         1396 c         1365 g          556 t
ORIGIN      
        1 cccccgcgcc gccgaccttc tgctactggg cgccggggcc ggcggcggcc gagtggtgcc
       61 ggatgcagaa cgacgccatc gcggagctgg tgagccgcca cccgacgcgg ctgctgggcg
      121 cgggtgggct cccgctgcag gcgcccgacc tcgcggtgaa agagctggag cgcgtggtgt
      181 ccgtgctccg cttcccggcg gtcgagatcg gcgccaacgt ggccgggctc gatctggacg
      241 acgcggccct cgccccggtg tgggacgcgg cggccgccct cgacgtcgca atcttcgtcc
      301 acccgcaggc gccgatcctg ggcgacccgc gcttccgcaa ggacaacctc acccaggtcg
      361 ccggcttccc gctcgagacc gcgctcgcga tgacccgggt gatcttcggc cgcgtgctcg
      421 accggtggcc gacgctgcgc tggtgcttcg cccacggcgg cggcgccttc gcctacatcc
      481 tgccgcgcct cgatcagggc tggaccgcca tcgccgacgc gcacgctgcg atcagccagc
      541 cgccgagcgc gtatgcccga cgcgtctggg tggactcgct caccctctcg ccccgcgtgc
      601 tcgccttcgc cctcgagacc ttcgggcccg agcgcatcgt gctgggcagc gactacccgt
      661 tcaagatggg ggtggccgat ccgatcgcgg cgctcgacgg cgtcaccctg gacgcggcca
      721 ctcggcagcg gcttttgggg gacaacgcgc gggagttcct ggccctgggc ccggcttgac
      781 cccggccgcg gcccagcgca atatccgccc atgaacttcg ggaccttcct cctgatgcag
      841 tcgccgtcgg cccggacgtc ccgggagatg tactcacggg cgatcgagat ggcccagtcc
      901 gccgagaccc tcgggttcca caacatctgg ctcgccgagc accacttctc gacctacggc
      961 tatctctcgc gtccggtcca gctcgccacc tacatcgcgg cccgcaccac ccgcatccgg
     1021 gtggggaccg cggtgatcgt ggtgccgctc caccacccgc tggtggtggc cgaggagatc
     1081 gcgacgctgg cgattctctc cggggaccgg ctcgacatcg gcctcgggcg tggctaccag
     1141 cactacgagt tcgagcgctt cgggctcgag ctcgagagcg ggcgggcgcg gtgggaggag
     1201 tcggtcgacg tcatcctgaa ggcgctcgcg ggcgggccgt tcagctacga gggcaagatc
     1261 tacaagatcc cggagaccat gatcttcccg cagatggagc agccgcggcc gccgatctgg
     1321 atcaccgccc agagcccgga ctcggtggag ggcgcggtgc gccgcggctt caacgtgctc
     1381 accggcggct tcggggtccc ggtcgagcgc atggccgagt tccgccggct cttcgaccgg
     1441 gtggtggccg aggtcaagcc gcccaccccg ctcgcggtcg gcgtgcagcg ggcggtctac
     1501 gtgaccacga gcgaggcgga cgcgcgggcc gccgcggagg aggcccgctg gaacatgcgg
     1561 gtgaccctga gcctccgcaa ccactacgag cgggtggaga acggccgggc catcgcggtg
     1621 cccgccccca aggagcccaa cgtcgacgac ctgctcgacc gcttcctggt catcggcacc
     1681 ccggagacct gcgtccgcca gatccagcgc ctgcagcgcg acgtgggcat cacccacttc
     1741 aactgcagct tctggttcgg cgatctcgag caggcccgag tgctgcgctc gatggagctc
     1801 ttcgcccgcg aggtgatgcc cgccttcgcg tagcgggccc ccgcccatgc cgaccccggg
     1861 tccgcgtcac gaatggccgg gcgcgtcgct cgcgcgcgtg ccgtactggg tctaccagga
     1921 cgaggacaac taccgggccg agctgcgccg catcttcgaa gggcccacct ggagctacgc
     1981 gtgcctcgag tcggaccttg agaagcccgg cgactaccgc accacgttcc tgggcgagat
     2041 gccgatcatc gtggtgcggg gcgcggcggg tgagatccac gccttcgaga atcgctgcgc
     2101 ccatcgcggc gcgctgatcg cgctcgacga cgggggctcg gcgggcagcc acttccagtg
     2161 cgtctatcac gcctggacct acgacctgcg gggcagcctg gtcggcatcg ccttcgagaa
     2221 gggcagcaac ggccgcggcg ggatgccgga gtcgttctgc aaggcggacc acgggccgcg
     2281 caagctgcgg accgctacgc tccacggcct cgtgttcgcg agcctctcgg ccgagacgcc
     2341 gccgatcgag gagtacctgg gcgcggaggt gctcgggcgc atccggcgcg tgctgcgcgg
     2401 cccgatgcgg gtcatgggcc gcttcaccca ggccctgccg aacaactgga agctctacgt
     2461 ggagaacgtc aaggacacct accacgcgag cctgctgcac gccttcttcg ggaccttccg
     2521 gatcacgcgc ctcacccagg ggggcgggat cctggtgagc ccggacggcg gccaccacgc
     2581 gagctacacc atcgaccgcg cggacgaccg caccagcacc gcctaccgcg agcagggcat
     2641 ccgctccgag aaggaccgtt atcgcctggt cgacccgagc ctgctcgact cggtggacga
     2701 gtggggcgac gggatccagc tgcagatcct caccgtgttc cccggcttcg tgctccagca
     2761 gatcaacaac tgcctggcgg tgcggcaggt ggtgccgaag ggcgtggggg gcatggagct
     2821 gcactggacc tacttcggct tcgccgacga ctccgacgac atgcggcgcc gccgcctcaa
     2881 gcaggccaac ctggtcggcc ccgccggcta cgtctccatg gaggacggct gcgtcggggg
     2941 tttcgtccag agaggcgtcg ccgcggcggg agacgaggtg tcggtggtca acatgggggg
     3001 cgagggcgcg gagtcgcagg agacgcgggc caccgaggcc tcggtgcgcg ggttctggaa
     3061 ggcgtatcgc cgctacatgg gggagtgacg tgctgggcga gcgcgtcgcg cagctcaacg
     3121 cggaatacgc gcgggccatc gacgacgatc gtctcgagca gtggcccgcg ctcttcaccg
     3181 acccgtgcct gtacaagatc acctcggcgg acaactaccg ccgcgggctc gaggcggggc
     3241 tgatctacgc cgactctcgc ggcatgctgc aggaccgcgt ggccgccctg cgcgaggcca
     3301 acatctacga gcgccaggcc taccgccacc tggtcggcct gcccgccatc cgccccgagt
     3361 cgaacggcgc gctgcacgcc gagaccccgt tcctggtggt gcgggtgatg cgcgacggga
     3421 ccatggacct cttcgccacc gggcgctacg tcgacgtcat ggtggacgag ggcggcacgc
     3481 tgcgcttccg tgagcgggtc gtcgtgtgcg acagcagccg cgtggacacg ctgctggcga
     3541 ttccgctctg acgcgcgctc gcggggcttc atgagcgggc ggcgcgcggc gggcgacggc
     3601 cggggggtgg cgcgggcggc cgccgcgggg ccggctccgt cacccgctcg ccggactcgc
     3661 cgctcttcga gcggcgcacc ggactcgggg ggcccactac gcgcggcccc gcggcaacca
     3721 cccacgccac cccccggccg gtcacacgcg agctcattcc tgcatcctcc gggcctagcc
     3781 aggtgaactg tcccgcgcga atcccggggc tttccaagtc gcgggtggtg gcggggattc
     3841 agtgtgcgaa gcagt
//
LOCUS       DATMCV010000597         2225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_166559, whole genome shotgun sequence.
ACCESSION   DATMCV010000597 DATMCV010000000
VERSION     DATMCV010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166559"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VNU03_13810"
     CDS             complement(<1..922)
                     /locus_tag="VNU03_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP75592.1"
                     /translation="MQCLGCQHENPPQAKFCLECGDRLALTCGRCRAELPGSAKFCLA
                     CGAPVGPGPQPRSPQSYTPKHLAERILTGRSTLEGERKQVTVLFADLKDSMELIADRD
                     PEEARKLLDPVLELMMAAVHRYEGTVNQVLGDGIMALFGAPLAHEDHAVRACYAALKM
                     QESVKRYAEEIFRSHGVLVQIRVGLNSGEVVVRAIDSDLHMDYVALGQTTHLAARMEQ
                     LAAPGTVLLAPTTLQLAEGYLQVTARGPVAVRGLPGPIEVYEVVGPGAVRSRLHAAAA
                     RGLTRFVGRDREVDELRRALERAQAGHGQIV"
     gene            1042..1995
                     /locus_tag="VNU03_13815"
     CDS             1042..1995
                     /locus_tag="VNU03_13815"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWP75593.1"
                     /translation="MLRFALLTVLLLGFVTPASAEYPERSLTIVVAYPAGGMVDIVAR
                     PMADSMKKTFTKGVAVLNRPGGGGSVGMAEVAQAKPDGYTVILAPNSTLVIHPQINEL
                     PYKTPDDYEPILNTITFYTLLTVKADAPWKTVQELMAAAKANPGKLRVGSPGEGTISH
                     LTLERWQIPGGFKTTHVPFSGWGETSPALLGGHIDAAMAQPGEVKPLVDAKKLRVLAV
                     ASPQRHPFFPDAPTGKEAGLGDGIGTWFVLTAPKGTPGPALKVLHNSAKAAMEEPSFV
                     ALMKSRGVDIDYKSGDKLRQELVREQKEWNDVLKRLGMIKK"
     gene            1996..>2225
                     /locus_tag="VNU03_13820"
     CDS             1996..>2225
                     /locus_tag="VNU03_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75594.1"
                     /translation="MLARAVPLVLLVAAGAYLILSLQLPFGAAARPGAGFYPVFVAVF
                     ALVVALGATTSAFRSAPGGDVAAVDLDAAARRR"
BASE COUNT          327 a          808 c          763 g          327 t
ORIGIN      
        1 cgacgatctg gccgtgcccg gcctgggctc gctcgagagc ccggcgcagc tcgtcgacct
       61 cccggtcccg gccgacgaag cgggtcagcc cgcgcgcggc cgccgcgtgc agccgcgagc
      121 gaaccgcgcc cggcccgacg acctcgtaga cctcgatcgg ccccgggagg ccccggaccg
      181 cgaccggccc tcgggccgtc acctggaggt agccctccgc gagttgcagc gtggtgggcg
      241 cgagcagcac ggtgccgggc gcggccagct gctccatgcg cgcggccagg tgcgtcgtct
      301 ggcccagcgc gacgtagtcc atgtgcagat ccgagtcgat cgcgcggacc acgacctctc
      361 ccgagttgag cccgacccgg atctggacga ggaccccgtg cgagcggaat atctcctcgg
      421 cgtaccgctt gaccgactcc tgcatcttga gcgccgcgta gcacgcgcgc accgcgtgat
      481 cctcgtgcgc gagcggagct ccgaagaggg ccatgatccc gtcacccagg acctggttca
      541 ccgtaccctc gtatcggtgg acggccgcca tcatgagctc gaggacggga tcgagcagct
      601 tccgcgcctc ctcggggtcg cggtcggcga tcagctccat cgagtccttc agatccgcga
      661 agaggacggt gacctgcttg cgctcgccct cgagcgtgct cctccccgtg aggatcctct
      721 ccgcgaggtg cttcggcgtg taggactggg gagagcgagg ctggggtccg ggcccgaccg
      781 gcgcgccgca cgccaggcag aacttcgccg acccgggcag ctcggcccgg catctcccgc
      841 aggtcagcgc cagacggtcg ccgcattcca ggcagaactt ggcctgcggt ggattctcgt
      901 gctgacaccc cagacactgc atgccggagg agcttaggct ggcccctccg aggtcgtcaa
      961 gggttgacgg cggcggtgag cctggcctag gatgcggctc acccggaccg ccgctctccg
     1021 tatccgagag ggaggacacg catgctccgc ttcgctcttc tcaccgttct gctcctcggg
     1081 ttcgtgacgc ccgcgtccgc cgagtatccc gagcgctccc tgacgatcgt cgtcgcctac
     1141 cccgcgggcg gcatggtcga catcgtggcc cgtccgatgg ccgactcgat gaagaagacg
     1201 ttcaccaagg gcgtcgcggt cctcaaccgg cccggcgggg gcggctcggt cgggatggcg
     1261 gaagtcgcgc aggcgaagcc ggacggctac accgtgatcc tcgcgccgaa ctcgaccctg
     1321 gtgatccatc cgcagatcaa cgagctgccg tacaagacgc cggacgacta cgagcccatc
     1381 ctgaacacga tcaccttcta cacgctcctc accgtgaagg cggacgcgcc gtggaagacg
     1441 gtgcaggagc tcatggccgc ggccaaggcg aatcccggca agctccgcgt cgggtcgccc
     1501 ggggagggca ccatcagcca cctgaccctc gagcggtggc agatcccggg cggcttcaag
     1561 accacccacg tgccgttctc cggctggggg gagacgagcc cggcgctgct gggcggccac
     1621 atcgacgcgg cgatggcgca gcccggcgag gtgaagcccc tcgtcgacgc caagaagctc
     1681 cgcgtcctgg ccgtcgccag cccccagcgg catcccttct tcccggacgc gcccaccggc
     1741 aaggaagcgg gactcgggga cgggatcggc acctggttcg tcctcaccgc cccgaagggc
     1801 acgccggggc ccgcgctgaa ggtgctccac aactcggcca aggccgcgat ggaggagccg
     1861 tcgttcgtcg cgctcatgaa gtcgcgcggc gtcgacatcg actacaagag cggcgacaag
     1921 cttcggcagg agctcgtgcg ggagcagaag gagtggaacg acgtgctcaa gcgcctcggg
     1981 atgatcaaga agtaggtgct cgcgcgcgcg gttcccctcg tgctcctggt cgccgcggga
     2041 gcctacctga tcctctcgct ccagctgccg ttcggggcgg ccgcccgtcc gggcgcggga
     2101 ttctatccgg tcttcgtcgc ggtcttcgcc ctggtcgttg ccctcggcgc gaccaccagc
     2161 gcgttccggt ccgccccggg cggcgacgtg gcggcggtcg atctggatgc ggccgcccgc
     2221 cgccg
//
LOCUS       DATMCV010000598         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_366352, whole genome shotgun sequence.
ACCESSION   DATMCV010000598 DATMCV010000000
VERSION     DATMCV010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366352"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VNU03_13825"
     CDS             <1..889
                     /locus_tag="VNU03_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75595.1"
                     /translation="ASELVETIFGGHSARVPGVDDHLENGVYSFQYHPELARDPSVND
                     GKGTLDYLRYAINLAERQNLWIATQRDLYQRMADYEALIFQTRNDGQTVTVTNPTTRP
                     IAAMVLEQRRPFASVWDGAEELVHVVRGAFVTIPPLPPGAAVTLRFAAEQPDAPLVRQ
                     PSTKGLTVLDARRDPATGETRIRVKVCRAQPLAVEGVDPDGVYRVQVDGEPERLILPR
                     VTRTIQALLSKQTAGAGPLGRRAAIPGTLRFLDLLVVGDEGRFVERTVRVAELPPAEA
                     AAARARILAAIPPRTGRVT"
     gene            complement(882..1229)
                     /locus_tag="VNU03_13830"
     CDS             complement(882..1229)
                     /locus_tag="VNU03_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HWP75596.1"
                     /translation="MIRPNLLAWQWSDYPAKHRDRVNLLLHVVAVPLFLAGTLLLIGA
                     ALRRSGLGVGLGLGCLAVAVVVEGRGHARERETPTPFAGPLDFASRFVVEQWITFPRF
                     VLTGAWSRNFRAR"
     gene            complement(1247..>2045)
                     /locus_tag="VNU03_13835"
     CDS             complement(1247..>2045)
                     /locus_tag="VNU03_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75597.1"
                     /translation="ALTRIEAVYQTESFPAATKSEAAGPGPCAGAACVFVKIMTTDGC
                     VWIRSGAPDVVNVELRLQGETVTMTLEQPDAAKVAKGPDPRGGNPREAKQAAARKRLD
                     ELRGKGMNIPYDPKVEGPRSAPEKAEAKDKGQTWHGTAYDAFRGSDVPVFNARVNRAS
                     GCVTKLEEILTYRARLASSKGKVAEGPPIECVGDACRDLDYGPEQIAACHLVNQGRRE
                     IAVGIVKSGSREPAISASVAPGRAVRLMHIMGCLSAQEIGRIEARYK"
BASE COUNT          289 a          773 c          696 g          287 t
ORIGIN      
        1 ggcgtccgag ctggtggaga cgatcttcgg cggccacagc gcgcgggtgc ccggcgtcga
       61 cgaccacctg gagaacggcg tctacagctt ccagtaccac cccgagctgg cccgcgaccc
      121 gtcggtaaac gacggcaagg gcacgctcga ctacctgcgc tacgcgatca acctcgccga
      181 gcgccagaac ctctggatcg ccacccagcg cgacctctac cagcgcatgg ccgactacga
      241 ggcgctgatc ttccagacgc ggaacgacgg ccagaccgtg accgtcacga acccgaccac
      301 ccggcccatc gccgcgatgg tgctcgagca gcgccgtccg ttcgccagcg tctgggacgg
      361 ggccgaggag ctggtgcacg tggtacgggg cgccttcgtc acgatcccgc cgctgccacc
      421 cggggccgcg gtgacactcc gcttcgccgc cgagcagccg gacgccccgc tggtccggca
      481 gccgagcacc aaggggctca ccgtcctcga cgcccgccgc gacccggcga ccggggagac
      541 gcggatccgg gtgaaggtct gccgcgcgca gccgctcgcg gtggagggcg tcgatcccga
      601 cggcgtctac cgggtgcagg tggacggaga gccggagcgc ctcatcctgc cgcgcgtgac
      661 gcgcacgatc caggcgctcc tgagcaagca gaccgcgggc gcgggaccgc tcggccggag
      721 ggccgccatc cccgggacgc tgcgcttcct cgacctgctc gtggtggggg acgaagggcg
      781 gttcgtggag cgcacggtca gggtcgccga gctgcccccc gcggaagcgg ccgcggcgcg
      841 ggcccggatc ctggccgcga tcccgccgcg caccgggcgc gtcacctagc tcggaagttc
      901 cggctccacg cgccggtcag gacgaagcgc gggaatgtga tccattgctc gaccacgaag
      961 cggctcgcga agtcgagcgg gcccgcgaac ggcgtcgggg tctcgcgctc gcgcgcgtgg
     1021 cctcgtccct ccaccaccac cgcgacggcc aggcacccga ggcccagccc gactccgagg
     1081 ccggagcgcc ggagcgcggc cccgatcagg agcaatgtcc cggccaggaa gagcggaacc
     1141 gccaccacgt gaagcagcag gttcacgcga tcccggtgct tggccgggta gtcgctccac
     1201 tgccacgcaa gcaggttcgg tctgatcatg ccgcgcctcc tcgtactcac ttgtagcgag
     1261 cctcgatgcg cccgatctcc tgggcactca ggcatcccat gatgtgcatc aggcgcacgg
     1321 ccctcccggg ggcgaccgag gcgctgatgg ccggctcgcg gctccccgac ttcacgatgc
     1381 ccaccgcgat ctcccggcgg ccctggttga ccaggtggca cgccgcgatc tgctcgggcc
     1441 cgtagtccag gtcccggcac gcgtcgccca cgcactcgat gggcgggccc tcggccacct
     1501 tccccttcga ggaggcgagt ctcgcccgat aggtgaggat ctcctcgagc tttgtcacgc
     1561 atccggacgc ccggttgacc cgggcgttga agacgggaac gtccgagccg cggaatgcat
     1621 cgtacgcggt gccgtgccag gtctgcccct tgtccttggc ctccgccttc tcgggagccg
     1681 agcgcggccc ctcgaccttc ggatcgtaag gaatgttcat gcccttcccg cgcagctcgt
     1741 cgagccgctt cctcgccgcc gcctgcttcg cctcccgagg gttcccccct cgagggtcgg
     1801 ggcccttcgc caccttcgcc gcgtcgggct gctcgagcgt catcgtgacc gtctcaccct
     1861 gcaggcgcag ctcgacgttc acgacgtccg gggcgccgct gcggatccag acgcacccgt
     1921 ccgtggtcat gatcttgacg aagacgcagg cggcgcccgc gcacgggccg gggccggcgg
     1981 cctcactctt ggtggcggcg gggaacgact ccgtctggta caccgcctcg attcgcgtga
     2041 gcgcg
//
LOCUS       DATMCV010000599         3895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_166637, whole genome shotgun sequence.
ACCESSION   DATMCV010000599 DATMCV010000000
VERSION     DATMCV010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166637"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VNU03_13840"
     CDS             <1..1365
                     /locus_tag="VNU03_13840"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HWP75598.1"
                     /translation="VELKQNIKRIWWRDYIQRRPDMMGLDASILMHPTVWKASGHVDN
                     FTDPMVDCLECRRRFRADKIDETPWVHYCPATKGNKFTIPAGEPCSHCGVARTLCPEC
                     GRGELTPPRQFNLMFKTFMGPVEEDASLTYLRPETAQGIFVNFDNVLQSMRRKLPFGI
                     GQIGKAFRNEITPGNFIFRTREFEQMEIEYFVNPHETVEGRPADEYWHERWIEDSMAW
                     FRRYGLSEENLRLREHEADELAHYAKRTADIEYRFPIGWSELMGIANRTDFDLKQHAK
                     FSGKALTYFDEERKEHVVPYVIEPSAGVDRALLAFLADAYREEEVRGEKRVVLRFHPE
                     LAPVSVAVLPLLKKRDDIVRTAHAIRDELSRDITAVYDDTAAIGRLYRRQDEVGTPYC
                     VTVDVQTVGDTDKGEPADGKVTIRDRDSMEQIRVEASRLAQMIGDLLRGTAWAEVAAG
                     AGRS"
     gene            complement(1370..3544)
                     /locus_tag="VNU03_13845"
     CDS             complement(1370..3544)
                     /locus_tag="VNU03_13845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HWP75599.1"
                     /translation="MAGDRRTKRAALRRRRWVRWLLMAVLVMVLVSALGVAASAFWVL
                     TILPGSLPSVAQLETFDASEGTKVYDDNDELITEFHVERRIFVPLAQIPKGLREAIIA
                     TEDARFYSHYGVDPTGIARAVYQNFRRGRLVEGGSTITQQLAKVLFLTPDKSLERKLK
                     EAVLALELERRYSKDRILEMYLNQIYFGHGAFGVEAAARTFFGKSVAELSLPEATLLA
                     GLPKAPSTYSPFEHPEAAKRRRATVLARMVDVGALAPPLAKKTAESTLGLIPPERRRT
                     TGQYYLEYVQQQLEAQYGADLVFKGGMQVYTTLSPVMQVKAERALREGLRALESRRVA
                     AAKDKAAPAPERPEGALLTIDPQTGYIKAMVGGYDFFKSEFNRAVQARRQPGSAFKAF
                     VYLAALESGQTPASVVDDSPVEFTTGRNGKPWKPDNYDRKFRGPITYQQALEESVNVA
                     AVKVLEKNGVRRTVDLARRLGVESPLGENLSIALGTSDLSLLEITSAYGALANQGAWM
                     RPTAIRYVLDSQRKLLEENTPQSKQVVSPELAYVMTYMLRGTIERGTGIAARALGRPA
                     AAKTGTTNDYSNAWFIGYTPQLVTGVWVGYDRPRSLGKDETGSRVAVPIWTAFMQEAL
                     AGTPPEDFPIPERVVLVPVDMNASGGCVRPVTMAFIAGTEPKEACGPARYAPGVSPPR
                     TEPPLLPAPSTPTAPSTPSAPVAPSAPAPASPPSTSVPQPQS"
     gene            3564..>3895
                     /locus_tag="VNU03_13850"
     CDS             3564..>3895
                     /locus_tag="VNU03_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWP75600.1"
                     /translation="MNTAPTADQQLARIRRGAAEIVVEAELRGKLERSLRTGTPLKVK
                     LGLDPTAPDLHLGHTVVLQKLRDFQDLGHQVVIIIGDFTGMIGDPTGRSETRRPLTWD
                     EIRENAET"
BASE COUNT          604 a         1301 c         1401 g          589 t
ORIGIN      
        1 gtggagctca agcagaacat caagcggatc tggtggcggg actacatcca gcgccggccc
       61 gacatgatgg gactcgacgc ctccatcctg atgcatccga ccgtgtggaa ggcgagcggc
      121 catgtcgaca atttcaccga ccccatggtc gactgtctcg agtgtcgccg gcgcttccgc
      181 gccgacaaga tcgacgagac gccgtgggtg cactactgtc ccgccaccaa ggggaacaag
      241 ttcaccatcc cggcgggtga gccgtgcagc cactgcggcg tcgcccggac actgtgtccc
      301 gagtgcggca ggggcgagct gaccccgccc cggcagttca acctcatgtt caagactttc
      361 atggggccgg tggaagaaga cgcgtccctc acctacctcc ggcccgagac ggcccagggc
      421 atcttcgtca acttcgacaa tgtcctgcag tcgatgcgac gcaagctacc cttcggcatc
      481 gggcagatcg gcaaggcctt ccgcaacgag atcacaccgg gcaacttcat cttccggacg
      541 cgcgagtttg agcagatgga gatcgagtac ttcgtcaacc cccacgagac ggtggagggg
      601 cgcccggccg acgagtactg gcacgagcgg tggatcgagg acagcatggc gtggttccgg
      661 cggtacggcc tcagcgagga gaacctgcgc ctgcgcgagc acgaggcgga cgagctagcg
      721 cactacgcca agcgcaccgc cgacatcgag taccgatttc ccatcggctg gagcgagctc
      781 atgggcatcg ccaatcgcac ggacttcgac ctcaagcagc acgccaagtt cagcgggaag
      841 gcgctcacct acttcgacga ggagcgcaag gagcacgtgg tgccctacgt catcgagccg
      901 tcggcggggg tggaccgggc cctgctcgcg ttcctggccg acgcctatcg cgaggaggag
      961 gtccggggcg agaagcgggt ggtgctgcgc ttccaccccg agctggcccc ggtgtcggtg
     1021 gcggtgctgc cgctgctcaa gaagcgcgac gacatcgtgc gcaccgcgca cgccatccgc
     1081 gacgagctat cccgcgatat caccgcggtc tacgacgaca ccgccgccat cggacggctc
     1141 tatcgccggc aggacgaggt gggcacgccc tactgcgtga cggtggacgt gcagacggtc
     1201 ggcgacaccg acaagggcga gccggccgac ggcaaggtga cgatccggga ccgcgactcg
     1261 atggagcaga tccgggtgga ggcgtcgcgg ctcgctcaga tgatcggcga cctgctgcgc
     1321 ggcaccgcgt gggccgaggt ggccgcgggg gccgggcgct cctagctccc tacgactggg
     1381 gctgtggcac gctcgtggac ggcggtgacg cgggcgcggg ggcgctcgga gcgacgggag
     1441 cgctcggggt gctcggagcg gtcggggtgc tgggggccgg cagcagcggc ggctcggtcc
     1501 gcgggggcga gacgcccggc gcgtaccgcg cggggccaca cgcctccttc ggctcggtgc
     1561 cggcgatgaa ggccatggtc accgggcgca cgcatccgcc gctcgcgttc atgtccaccg
     1621 gcaccagcac cacccgctcc gggatcggga agtcctccgg aggtgtgccc gcgagcgcct
     1681 cctgcatgaa cgcggtccag atcggcaccg cgacgcggga gccggtctcg tccttgccca
     1741 ggctgcgggg ccggtcgtag ccgacccaca ccccggtcac cagctgcggg gtgtagccga
     1801 tgaaccacgc gttcgagtag tcgttggtgg tgccggtctt ggccgcggcg ggccgcccga
     1861 gcgcgcgggc cgcgatcccg gtgccgcgct cgatcgtccc gcgcagcatg taggtcatca
     1921 cgtaggccag ctccggcgag acgacctgct tgctctgcgg ggtgttctcc tcgaggagct
     1981 tgcgctgcga gtcgagcacg tagcggatcg cggtgggtcg catccacgcg ccctggttgg
     2041 cgagcgcgcc gtaggcggag gtgatctcga gcaggctcag gtcggacgtg ccgagggcga
     2101 tcgacagatt ctcgccgagc gggctctcca ccccgagccg ccgggcgagg tccaccgtgc
     2161 ggcggacccc gttcttctcg agcaccttca ccgcggccac gttcaccgac tcctcgagcg
     2221 cctgctggta ggtgatcggc ccgcggaact tccggtcgta gttgtcgggc ttccagggct
     2281 tgccgttgcg gccggtggtg aactcgaccg gtgagtcgtc gaccacgctg gccggggtct
     2341 ggccggactc gagcgcggcc aggtagacga aggccttgaa ggccgacccg ggctgccggc
     2401 gcgcctggac cgcgcgattg aactcgctct tgaagaagtc gtagccgccc accatcgcct
     2461 tgatgtagcc ggtctggggg tcgatggtga ggagcgcacc ctcgggtcgc tccggcgccg
     2521 gcgcggcctt gtccttggcc gccgcgaccc ggcgcgactc gagcgccctg agcccctcgc
     2581 gcagggcacg ctccgccttg acctgcatca ccggggagag ggtggtgtag acctgcatgc
     2641 cgcccttgaa gacgaggtcg gccccgtact gagcctcgag ctgctgctgg acgtactcga
     2701 ggtagtactg gccggtggtg cgccggcgct cgggcgggat caggccgagc gtgctctccg
     2761 cggtcttctt ggccagcggg ggagcgagcg cgcccacgtc caccatgcgg gcgagcacgg
     2821 tggcgcgccg gcgcttggcc gcttccggat gctcgaacgg cgagtaggtc gagggcgcct
     2881 tgggcagccc ggcgagcagg gtggcctcgg ggaggctcag ctcggcgacc gacttgccga
     2941 agaaggtgcg cgcggccgcc tctaccccga aggcgccgtg gccgaagtag atctggttca
     3001 ggtacatctc gaggatgcgg tccttggagt agcgccgctc gagctcgagg gcgagcaccg
     3061 cctccttgag cttgcgctcc aggctcttgt ccggagtgag gaagagcacc ttggcgagct
     3121 gctgggtgat ggtgctgccg ccctccacga ggcgcccgcg ccggaagttc tggtagaccg
     3181 cgcgggcgat gccggtcgga tcgacgccgt aatgtgagta gaagcgcgcg tcctcggtgg
     3241 cgatgatcgc ctcgcgcagc cccttcggga tctgggcgag cggcacgaag atgcgacgct
     3301 cgacgtggaa ctcggtgatc agctcgtcgt tgtcgtcgta gaccttggtg ccctcgctcg
     3361 cgtcgaaggt ctcgagctgg gccaccgacg ggagcgagcc gggcaggatg gtgaggaccc
     3421 agaacgcgga ggcggccact ccgagggcgc tgacgagaac catcaccagt accgccatga
     3481 gcagccagcg gacccagcgg cggcgccgga gcgcggcgcg tttggtgcgt cggtctcccg
     3541 ccatggattg ctattataca ggcgtgaaca cggcccctac cgcggatcag cagctggccc
     3601 gcatccgccg gggcgccgcc gagatcgtgg tggaggccga gctgcgcggc aagctcgagc
     3661 gctccttgcg caccggcacg ccgctcaagg tcaagctcgg cctcgatccg accgcgcccg
     3721 acctgcatct gggccacacc gtcgtgctcc agaagctgcg cgacttccag gacctgggcc
     3781 accaggtggt catcatcatc ggggatttca ccgggatgat cggcgatccc accggacgct
     3841 ccgagacgcg gcgtccgctc acgtgggacg agatccgcga gaacgcggag accta
//
LOCUS       DATMCV010000600         5173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_828991, whole genome shotgun sequence.
ACCESSION   DATMCV010000600 DATMCV010000000
VERSION     DATMCV010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828991"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VNU03_13855"
     CDS             <1..357
                     /locus_tag="VNU03_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75601.1"
                     /translation="MTRTETGGALVLCQGERNWFEAVDTPFVVQRLRLTVEDTALRAV
                     ELHFVGDYREPLDPRTLEAERDLLYCRVRGGLFRARFGRVALQQLAPFLLDTGEGLAL
                     LVSGARHPVRAAAAVI"
     gene            383..1285
                     /locus_tag="VNU03_13860"
     CDS             383..1285
                     /locus_tag="VNU03_13860"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75602.1"
                     /translation="MSERVGFVGLGTMGLPMATNLAKGGVTVVAYDASPAAQAAAARL
                     PGATVAASVADAAAQCAVLFTCLPNDAIVREVYLGAGGIASAARRGLVTCDCSTVSPE
                     VTTALHAALKERGVDHMDTPMLGSQPQAVDGQIFFIVGGEAGRLPAIAPYLEIMGKMH
                     MYVGGSSMANRVKLIHNGLAAVTSVAVAEALAVCVQSGVDPSTFYDVVRSGGGMAYGT
                     YFDRRAKRIFDGEFSPTFMLELMRKDAMLALALARSVQVPAPMLEETKRTYDEAVDSG
                     WGREDFSAVTHVVEKRIGRRLSGR"
     gene            1362..1829
                     /gene="bcp"
                     /locus_tag="VNU03_13865"
     CDS             1362..1829
                     /gene="bcp"
                     /locus_tag="VNU03_13865"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HWP75603.1"
                     /translation="MPLVEPGRKAPAFALPDQDGKIHRLRDYAGRPLILYFYPKDDTP
                     GCTREACAFRDALPGFKKGRAAVLGVSVLDTASKARFARKHDLNFPLLADPEHEVADK
                     YGVWQKKSLYGRTYMGLARTTYLIGPDGKVARRWDNVKVDGHAADVLRAVGEL"
     gene            1844..2698
                     /locus_tag="VNU03_13870"
     CDS             1844..2698
                     /locus_tag="VNU03_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWP75604.1"
                     /translation="MSVVVGAYEDAASAVEALAQVGGKRPAAVLPRGAVDDEARLRLR
                     QAQVECLTTLDGGDMDAARWLAATLAQAQGWEPADPRGAPTGGSAPGDVIGWCELRIG
                     SGALTTDRPITRLHGAQQYIASFNLLGQGRLNQACGDLLYRRLMDEGVALDEVFDVVV
                     CSESKAVGMVQVVAECFGQDRYVVLRKGLKNYMPRRPREPLVEDAVSITTAGRQALVL
                     DPLDWPLLEGRRVLLVDDVIATGGTARAASRLLERAGARVTAAATVLLKGPEPDLPRL
                     VVLARPLL"
     gene            2740..3408
                     /locus_tag="VNU03_13875"
     CDS             2740..3408
                     /locus_tag="VNU03_13875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWP75605.1"
                     /translation="MGQMINLTAEDQHRLSAYRAAPSGSPKGGLVVVQEIFGVNHHIQ
                     GLCDRFAADGYVALAPAIFDRVEPGISLGYTADDIERGRSIRGKVDIADMVRDVRAAV
                     EALKAEGLPTGVVGYCLGGTLAWLACTRVDGVKAAVGYYGGGIAETASETPRCPVLLH
                     FGETDQGIPPEHWARIRAAHPEVPIHTYPAGHGFSCDERGSYHEPSARLARQRTLGFF
                     TALL"
     gene            3414..3911
                     /locus_tag="VNU03_13880"
     CDS             3414..3911
                     /locus_tag="VNU03_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWP75606.1"
                     /translation="MGDIPAPFDAYRDAVRPEWIDDNRHMNMGYYVVVFDLATDEWMR
                     FVGLDRAHRRAREVTTFCLETHVTYHQEVRERDPLRFTTQLLDFDAKRIHYIHQMYHG
                     ESGYLAATNEVMSLHVSQQTRRGAPMAPEILERLGRIRAAHELLPRPPQVGRVMGLGA
                     RPTTR"
     gene            complement(3948..>5173)
                     /locus_tag="VNU03_13885"
     CDS             complement(3948..>5173)
                     /locus_tag="VNU03_13885"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWP75607.1"
                     /translation="DLDRFKVVNDSMGHGVGDQLLIGVSQRLAACLRPEDTIARLGGD
                     EFAILLEDVKDDKGPISVADRLTAELQQPFMVEGREVVITVSIGIAMSTARRMTPEDI
                     LRDADLAMYHAKGKGKARYEIFDKSMNAPAQERMDLELDLRNAVARGEFVLHYQPVVD
                     LPTGRISEVEALVRWKHPQRGLLFPADFIGLSEETGLIVALGRWVLHEACRQTRQWQL
                     ASPDSKLAISVNLSARQLQQPGLVEEIAAVLRETRLDAADLRLEITETVVMHDAPTTL
                     AKLEALKALGVQLAIDDFGTGYSSLGYLKRFPVDTLKIDRSFVKGIGDDVEDSAIVRA
                     VITVAKSLNLSVTAEGIETAEQLEHLRALGCDHGQGYFFAKPMASERVPALLLAVSPW
                     GPAAATNGHTTNGHN"
BASE COUNT          714 a         1806 c         1911 g          742 t
ORIGIN      
        1 atgacccgca ccgagaccgg cggcgccctc gtcctctgcc agggcgagcg aaactggttc
       61 gaggccgtcg acaccccctt cgtggtgcag cgccttcgcc tcacggtgga ggacacggcg
      121 ctccgggcgg tggagctcca cttcgtgggc gactatcggg agccgctcga tccgcgtacg
      181 ctcgaggccg agcgggacct gctctactgc cgcgtccgcg gcgggttgtt ccgggcgcgc
      241 ttcggccgcg tggcgctcca gcagctcgcg ccattcctgc tggataccgg ggagggcctg
      301 gccctgctcg tgtccggcgc ccgccatccg gtgcgtgccg ccgcggcggt catctagcgt
      361 cacatcgagt cgaggaacga ccatgagcga gcgagtcgga ttcgtcgggc tgggcacgat
      421 ggggctgccg atggccacca acctcgccaa gggcggcgtg accgtggtgg cctacgacgc
      481 gagccccgcc gcgcaggcgg cggccgcgcg gctccccggc gcgacggtgg ccgcgtcggt
      541 ggcggacgcg gcggcccagt gcgcggtgct cttcacctgc ctgcccaacg acgcgatcgt
      601 gcgcgaggtc tacctgggcg cgggcggcat cgcgtccgcg gcgcgccggg ggctcgtgac
      661 gtgcgactgc tcgacggtga gcccggaggt caccaccgcg ctgcacgccg cgctgaagga
      721 gcggggcgtc gatcacatgg acacgccgat gctgggctcg cagccccagg cggtggacgg
      781 gcagatcttc ttcatcgtgg gcggggaggc cggccgcctc cccgcgatcg cgccgtacct
      841 cgagatcatg ggcaagatgc acatgtacgt gggcggctcc agcatggcca accgggtcaa
      901 gctcatccac aacggcctgg ccgcggtgac ctcggtggcg gtcgcggagg cgctcgcggt
      961 gtgcgtgcag tccggcgtgg acccgagcac gttctacgac gtggtgcgca gcggcggcgg
     1021 catggcctac ggcacctact tcgaccggcg ggccaagcgg atcttcgacg gcgagttctc
     1081 gcccacgttc atgctcgagc tgatgcgcaa ggacgcgatg ctcgcgctcg ccctggcccg
     1141 atcggtccag gtgccggcgc cgatgctgga agagaccaag cgcacctacg acgaggcggt
     1201 cgacagcggg tggggacggg aggacttctc ggcggtcacc cacgtggtgg agaagcgcat
     1261 cggtcgccgg ctctcggggc ggtagcgcct ccccccggcc gggagtagga tgggcgccgg
     1321 cgcgcggccg ggcgccgcat cacctaccca ggaggatttc gatgcctctc gtcgagccgg
     1381 gacgcaaggc ccccgccttc gcgctgcccg atcaggacgg caagatccac cggctgcggg
     1441 actacgcggg ccggccgctg atcctctact tctacccgaa ggacgacacc ccgggctgca
     1501 cccgggaggc gtgcgccttt cgcgacgcgc tgcccggctt caagaagggc cgggccgcgg
     1561 tgctcggggt gagcgtgctc gacaccgcca gcaaggcgcg cttcgcgcgc aagcacgacc
     1621 tgaacttccc gctgctcgcc gacccggagc acgaggtggc cgacaagtac ggcgtgtggc
     1681 agaagaagag cctctacggt cggacgtaca tgggcctggc ccgcacgacc tacctcatcg
     1741 gcccggacgg caaggtcgcg cgccgctggg acaacgtgaa ggtcgacggc cacgcggccg
     1801 acgtcctccg ggccgtcggc gagctctgag aggccccgcg atcgtgtcgg tcgtcgtcgg
     1861 cgcgtacgag gatgcggcct ccgcggtgga ggcgctcgcc caggtgggcg gcaagcggcc
     1921 cgccgcggtg ctgccgcgcg gcgcggtcga cgacgaggcc cggctgcggc tgcgccaggc
     1981 ccaggtcgag tgcctcacca cgctcgacgg gggcgacatg gacgcggcgc ggtggctcgc
     2041 ggcgacgctc gcccaggccc agggctggga gcccgccgat ccgcgggggg cgccgacggg
     2101 cgggagcgcg cccggcgacg tgatcggctg gtgcgagctg cgcatcggct ccggcgcgct
     2161 caccaccgat cgaccgatca cccggctcca cggagcgcag cagtacatcg cgtcgttcaa
     2221 cctgctcggc cagggccggc tcaaccaggc ctgcggggac ctgctctatc gccggctgat
     2281 ggacgagggc gtcgcgctcg acgaggtctt cgacgtggtg gtgtgctcgg agtcgaaggc
     2341 ggtgggcatg gtgcaggtgg tcgcggagtg cttcggccag gaccgctacg tggtgctccg
     2401 caagggcctc aagaactaca tgccgcgccg gccgcgcgag cccctggtgg aggacgcggt
     2461 gagcatcacc accgcgggcc gtcaggcgct ggtgctggat ccgctcgact ggccgctgct
     2521 ggaaggccgc cgggtgctgc tggtggacga cgtcatcgcc accgggggca ccgcccgcgc
     2581 ggccagccgc ctgctggagc gggcgggcgc gcgcgtgacc gccgcggcga ccgtgctgct
     2641 gaagggcccc gagcccgacc tgccccggct cgtggtgctc gcgcgcccac tcctctgagg
     2701 gcagtcctct ctccaacctt tccgacaagg agccaaccga tgggccagat gatcaacctc
     2761 accgccgaag accagcaccg cctctccgcc tatcgcgcgg cgcccagcgg gagcccgaag
     2821 ggcgggctcg tcgtcgtcca ggagatcttc ggggtcaacc accacatcca gggcctgtgc
     2881 gatcgcttcg cggccgacgg ctacgtggcg ctggcgcccg cgatcttcga ccgggtggag
     2941 cccggcatca gcctcggcta caccgccgac gacatcgagc gcggccgctc gatccgcggc
     3001 aaggtcgaca tcgcggacat ggtgcgcgac gtgcgggccg cggtggaggc cctcaaggcc
     3061 gaggggctgc cgaccggggt ggtcggctac tgcctgggcg gcaccctggc ctggctcgcc
     3121 tgcacgcgcg tcgacggggt gaaggcggcg gtgggctact acggcggcgg catcgcggag
     3181 accgcgagcg agacgccgcg ctgcccggtg ctcctgcact tcggggagac cgaccagggg
     3241 atcccgccgg agcactgggc gcgcatccgc gccgcccacc cggaggtgcc gatccacacg
     3301 tatccggccg gccacgggtt cagctgcgac gagcggggga gctaccacga gccgagcgcg
     3361 cggctggccc gtcagcgcac cctcggcttc ttcaccgcgc tgctctagtc gcggtggggg
     3421 acattcccgc gcccttcgac gcgtaccggg acgccgtccg cccggagtgg atcgacgaca
     3481 accggcacat gaacatgggc tactacgtgg tggtgttcga cctcgccacc gacgagtgga
     3541 tgcggttcgt gggcctggac cgggcgcacc ggagggcgcg ggaagtgacc acgttctgtc
     3601 tcgagaccca cgtcacctac catcaggagg tgcgcgagcg ggacccgctg cgcttcacca
     3661 cgcagctgct ggatttcgac gccaagcgca ttcactacat ccaccagatg taccacggcg
     3721 agtcgggcta cctcgcggcc accaacgagg tgatgtccct gcacgtgagc cagcagacgc
     3781 ggcgcggcgc ccccatggcg ccggagatcc tggagcggct cggtcgcatc cgggccgcgc
     3841 acgagctgct gccgcgtccg ccccaggtgg ggcgcgtgat ggggctaggg gcgaggccga
     3901 cgacgcggta acgcgacgcg gcgggtcagg tcttggagac ctgattccta gttgtggccg
     3961 ttggtggtgt ggccgttggt ggcggcggcg gggccccagg gcgagacggc cagcagcagc
     4021 gcgggcacgc gctcgctcgc catgggcttg gcgaagaagt agccctggcc gtggtcgcag
     4081 ccgagcgcgc gcaggtgctc gagctgctcg gcggtctcga tcccctccgc ggtgaccgag
     4141 aggttgaggc tcttggccac cgtgatgacc gcgcgcacga tcgcgctgtc ctccacgtcg
     4201 tcgccgatgc ccttcacgaa cgagcggtcg atcttgaggg tatcgaccgg gaaccgcttg
     4261 aggtagccga gcgaggagta gccggtcccg aagtcgtcga tggcgagctg gaccccgagg
     4321 gccttgaggg cctcgagctt ggcgagcgtg gtcggcgcgt cgtgcatgac cacggtctcg
     4381 gtgatctcga ggcgcaggtc ggccgcgtcg agccgcgtct cgcgcagcac cgccgcgatc
     4441 tcttccacga ggccgggctg ctggagctgc cgggccgaga ggttcacgct gatcgcgagc
     4501 ttgctgtccg gggaggcgag ctgccactgc cgggtctgcc ggcacgcctc gtggagcacc
     4561 cagcggccga gggccacgat cagcccggtc tcctcggaga ggccgatgaa gtcggccggg
     4621 aagaggagcc cgcgctgcgg atgcttccac cggacgagcg cctccacctc gctgatgcgg
     4681 ccggtgggca ggtccaccac cggctggtag tgcagcacga actcgccccg cgccaccgcg
     4741 ttgcgcaggt cgagctcgag gtccatccgc tcctgggccg gcgcgttcat actcttgtcg
     4801 aagatctcgt agcgcgcctt gcccttgccc ttggcgtggt acatggcgag gtcggcgtcg
     4861 cgcaggatgt cctcgggggt catgcgccgc gcggtgctca tcgcgatgcc gatgctcacc
     4921 gtgatcacga cctcgcgtcc ctcgaccatg aagggctgct gcagctcggc ggtgaggcgg
     4981 tcggccaccg agatgggccc cttgtcgtcc ttcacgtcct cgagcaggat cgcgaactcg
     5041 tcgccgccga gccgcgcgat cgtgtcctcg gggcggagac aggcggccag ccgctggctc
     5101 accccgatca ggagctggtc gccgacgccg tgccccatgc tgtcgttgac caccttgaac
     5161 cggtcgaggt cga
//
LOCUS       DATMCV010000601         5038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_694582, whole genome shotgun sequence.
ACCESSION   DATMCV010000601 DATMCV010000000
VERSION     DATMCV010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694582"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4246
                     /locus_tag="VNU03_13890"
     CDS             <1..4246
                     /locus_tag="VNU03_13890"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWP75608.1"
                     /translation="ELNISDLLARPRSQSEGSPFSLDHLGVKDGAILFEDRTLTPPRT
                     WRAEALTVEAGALSSVSPEPRGHVRVTTTVAGAPFSLDATGIALRPLQAKAHVSLRDV
                     DATLANLYLPPDTMVTLERAVVGAEVDATLDAQGGVGLDGQARITNLVARRRGVDASL
                     FTVPALTFALTSGKSPEGRLTGRVEVNGRATVFDPRPGKSNRFEIERLRVVADGLDAT
                     GRSSARLTASAGLPGGGALDVQGTARATPVAAEVRTRISRVDLAFWAPYFSLPVEFTG
                     MAETDLTLDTTPAGPRVRGSALVKGVTVSDGTRRLAAADQLELSGLDAQWPRTKVERV
                     RLSRPRARIGRDRDGRLTIAELVESIKKPAGESAAAAPVDKPTPLPPDFAIEVGEVSV
                     EDGRLRLDDATVEPAAVFRLAPIRFTARSLTWPSRGPATLQLSAGTPEGGTIETQGTV
                     VLDPVRFDLRTRLVNIAVAPYRAYAPLAAKVEGHVEGDVTAKGTVGGGKTELSAKGSL
                     ALADLAFIDGDRPILTVGRLELAGLDYTWPATATIDRVHMRKSWALLERRPDGSMPIT
                     GILTPLRRAPPAGAPAPTPPVAPSPPASPPPAASPTTTSSPSPDIKVTVREVLFDEGG
                     ATVVDVAVNPVARLEVSGVRLSARDFAWPARAPVPVQLEAPTPGAGRITARGTLDLVA
                     RSLQMQLTPAGVDLGPAQPYLPVRGRLTGQASGDLALKATLEPFSFTARGTAAIADMA
                     LGDASKPLMTAARLEATGIDYAWPATVTVDRVELQKPWAQIEQAADGSFPLRALLEPP
                     APPPGAPPRREGPAAGGAASPLDLRVRRAGVDGGVFEIIDSTVRPTARAQIHDARLTI
                     RNFTWPAREPSALRLRATTGTGGTVDARGEMRLDTQAVDLQLAIKQLDVAIAQAFVPG
                     RASLAGKLDADLRVKGTLSPLSVAATGRVAVDDPILGDGQRMLGYVKRVDMDGIDADW
                     PRRVTIRRVTLDQPWALVEREDNGGFPLIDLLTPTAPAGAAPAPAPAPRGGSAPPSRP
                     PADGALPVVTLGELAVAEGFVRFVDRTTKPAFTEEASRIALNGRGLTTARTGKGQLTL
                     AGRLTGGAPFELKGTMGGLGGPINLDLEGKLTDFPLPRVNPYANQLIGWEARRGAFGT
                     TIRYRVVDDALTASNDIVLGQPDFAPSRSGDEVRDRVGVPLGTLIALLKNAKGEVRLS
                     VPVTGNVATRQFDFTDAFWAAVRKTAVGVMALPVSWVGKIFYSGDDRVETIQIWPVYF
                     EPGTTRFTEGFDRHAERLGGFLRDAPGVNLAMKSVLTVDDIAALKRDVVQQRIEGAAR
                     ETPGGAAAAAARLYAERHPGQPVPGGIDAIVSDLVKDEPNPDAAARTLANRRMETARA
                     QLQKTVKIAPERLRVTEGLVPVEGSGLGRVEFEIAP"
     gene            complement(4270..4833)
                     /locus_tag="VNU03_13895"
     CDS             complement(4270..4833)
                     /locus_tag="VNU03_13895"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP75609.1"
                     /translation="MATAQTPRGINHVVLNVRDIEVSHRFWTEIMGFRCVAQLKPRPD
                     RVRPKMRFYSGVSESGDVTHHDLALAEAPKREGETEGEGETWSLMRGGVGLNHVAIAW
                     PDRESWLKQVEHLREKGVTFHRRINHGMTHSVYISDPDGHGIEVLYELPREVWEHDID
                     AAQNYAEVLPTEGPASMVDRVDNPVFS"
     gene            complement(4853..>5038)
                     /locus_tag="VNU03_13900"
     CDS             complement(4853..>5038)
                     /locus_tag="VNU03_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWP75610.1"
                     /translation="EIKRVLADGDYVILHVHAVRTPGERGSAIVDVFKLENGKIVEHW
                     DVVQPIPEKSANGNGMF"
BASE COUNT          661 a         1916 c         1797 g          664 t
ORIGIN      
        1 cgagctgaac atctcggacc tgctcgcgcg cccacgctcg cagtcggagg gctcgccgtt
       61 ctcgctggac cacctcgggg tcaaggatgg cgcgatcctg ttcgaggacc gcaccctgac
      121 cccgccccgc acctggcgcg ccgaggccct caccgtcgag gccggcgcgc tctccagcgt
      181 gagcccggag ccgcgcggcc acgtgcgggt caccaccacg gtggcgggtg ccccgttctc
      241 gctggacgcc accggcatcg cgctgcgccc cctgcaggcg aaggcccacg tcagcctgcg
      301 cgacgtcgac gccaccctcg ccaacctgta cctgccgccc gacaccatgg tgacgctgga
      361 gcgcgcggtg gtgggcgcgg aggtcgacgc cacgctcgac gcgcagggcg gggtcggact
      421 cgacggccag gcgcgcatca ccaacctggt ggcgcgccgc cgtggggtcg acgcgtccct
      481 cttcacggtt cccgcgctga ccttcgcgct gaccagcggc aagtcaccgg agggccgcct
      541 caccggccgg gtggaggtga acgggcgggc caccgtcttc gatccccggc ccggcaagtc
      601 caaccgcttc gagatcgagc gcctccgggt cgtggccgac gggctcgacg cgacgggccg
      661 ctcctccgcg cgcctcaccg ccagcgccgg cctgcccggc ggcggcgcgc tcgacgtgca
      721 gggcaccgcg cgcgccaccc cggtggcggc cgaggtccgc acccggatca gccgggtcga
      781 cctcgcgttc tgggcgccct acttctcgct gccggtggag ttcaccggca tggccgagac
      841 cgatctcacc ctcgatacca cgcccgcggg cccccgcgtg cgcgggagcg cgctggtgaa
      901 gggcgtcacg gtgtccgacg gcacgcggcg cctggccgcc gccgatcagc tcgagctgag
      961 cggcctcgac gcccagtggc ccaggacgaa ggtcgagcgg gtgcggctca gccggccccg
     1021 cgcccgcatc gggcgcgatc gcgacggtcg cctcacgatc gcggagctgg tggagtcgat
     1081 caagaagccc gcgggcgaga gcgccgcggc ggccccggtc gacaagccca ctccgctgcc
     1141 gccggacttc gcgatcgagg tgggcgaggt gtccgtcgag gacgggcgcc tccgtctcga
     1201 cgacgccacc gtggagccgg ctgcggtctt ccgcctcgcc ccgatccgct tcaccgcccg
     1261 ctcgctcacg tggccgagtc gcgggccggc cacgctccag ctgagcgcgg gcacgcccga
     1321 gggcggcacg atcgagacgc agggcaccgt ggtgctcgat ccggtgcgct tcgatctgcg
     1381 cacccggctg gtcaacatcg cggtcgcgcc ctatcgggcc tacgctccgc tcgccgcgaa
     1441 ggtggagggg cacgtggagg gcgacgtcac cgccaagggc acggtgggcg gcggcaagac
     1501 cgagctgtcc gcgaagggct cgctcgccct ggccgacctg gccttcatcg acggggaccg
     1561 cccgatcctc acggtgggcc gtctcgagct ggccggcctc gactacacgt ggcccgccac
     1621 cgccaccatc gaccgggtgc acatgcgcaa atcgtgggcc ctgctcgagc gccggcccga
     1681 cggctccatg ccgatcaccg gcatcctcac cccgctgcgc cgcgcaccgc cggctggcgc
     1741 gccggcgccc acgcccccgg tggcgccatc gccgccagca tcgccgccgc ctgcggcgtc
     1801 accgaccacg acgtcgtcgc cgtcccccga tatcaaggtg accgtgcgcg aggtgctctt
     1861 cgacgaaggc ggcgccaccg tggtggatgt ggcggtgaat cccgtcgcgc ggctcgaggt
     1921 gagcggggtg cggctcagcg cccgcgactt cgcgtggccg gcgcgggcgc cggtgccggt
     1981 gcagctcgag gcgccgaccc cgggcgcggg ccgcatcacc gcccgcggca cgctcgacct
     2041 ggtcgcgagg agcctccaga tgcagctgac cccggccggg gtggacctcg ggccggccca
     2101 gccctatctg ccggtgcgtg ggcggctcac cgggcaggcc agcggcgacc tcgcgctcaa
     2161 ggccacgctc gagcccttca gcttcacggc gcgcggcacc gcggcgatcg cggacatggc
     2221 cctgggagat gcgagcaagc cgctgatgac cgcggcccgg ctcgaggcca ccggcatcga
     2281 ctacgcgtgg cccgccaccg tcaccgtgga tcgcgtcgag ctccagaagc cgtgggcgca
     2341 gatcgagcag gccgccgacg gctcgttccc gctccgcgcg cttctggagc cgcccgcccc
     2401 gcccccgggc gctccgccgc ggcgcgaggg acccgccgcg ggcggcgccg cctcgccgct
     2461 cgacctgcgc gtgcggcggg cgggcgtcga cggcggcgtg ttcgagatca tcgactccac
     2521 cgtgcgtccc accgcgcggg cgcagatcca cgacgcgcgg ctgacgatcc gcaacttcac
     2581 gtggcccgcg cgcgagccgt cggccctgcg cctgcgcgcc accaccggca ccggcggcac
     2641 ggtggacgcg cgcggcgaga tgcggctcga cacgcaggcc gtcgatctcc agctcgcgat
     2701 caagcagctc gacgtggcca tcgcccaggc cttcgtgccc gggcgcgcca gcctggccgg
     2761 caagctcgac gcggacctgc gcgtgaaggg cacgctctcg ccgctctccg tcgcggcgac
     2821 cgggcgggtg gcggtggacg acccgatcct gggcgacggc cagcggatgc tgggctacgt
     2881 gaagcgagtg gacatggacg ggatcgacgc ggactggccg cgccgggtca cgatccgtcg
     2941 cgtgaccctc gaccagccgt gggccctcgt ggagcgcgag gacaatggcg gatttcccct
     3001 gatcgacctg ctcaccccga ccgcgcccgc cggcgccgcc cccgccccgg cgcccgcgcc
     3061 ccgcggcggg agcgcgccac ccagccgccc acccgccgac ggagcgctgc ccgtcgtcac
     3121 gctgggcgag ctggcggtcg cggaagggtt cgtacgcttc gtcgatcgca ccaccaagcc
     3181 cgccttcacc gaggaggcct cgcgcatcgc gctgaacggc cgcgggctca ccaccgcgcg
     3241 caccggcaag gggcagctca ccctggcggg gcggctcacc ggcggcgctc cgttcgagct
     3301 gaaggggacg atgggcggcc tgggagggcc catcaacctc gatctggagg gcaagctcac
     3361 cgacttcccc ttgccgcgcg tgaacccgta cgccaaccag ctgatcggct gggaagcgcg
     3421 gcgcggcgcc ttcggcacca ccatccgcta ccgggtggtc gacgacgcgc tcaccgccag
     3481 caacgacatc gtgctcggcc agcccgactt cgccccgtcg cgcagcggcg acgaggtgcg
     3541 cgaccgggtc ggcgtcccgc tcggcacgct gatcgccctg ctcaagaacg ccaaggggga
     3601 ggtgcgcctg tcggtgccgg tcaccggcaa cgtggccacc cgccagttcg atttcacgga
     3661 cgccttctgg gccgcggtcc gcaagaccgc ggtcggagtg atggcgctgc cggtgagctg
     3721 ggtcggcaag atcttctaca gcggtgacga tcgcgtcgag accatccaga tctggccggt
     3781 gtacttcgag cccggcacca cccgcttcac cgaaggcttc gaccggcacg ccgagcggct
     3841 gggcggcttc ctgcgggacg cgcccggggt gaacctggcc atgaaatcgg tgctgaccgt
     3901 cgatgacatc gcggcgctca agcgcgacgt ggtgcaacag cgcatcgagg gcgccgcacg
     3961 cgagaccccg ggcggggccg ccgccgcggc ggcgcgtctc tacgccgagc ggcaccccgg
     4021 gcagccggtg ccgggcggga tcgacgcgat cgtgagcgac ctggtcaagg acgagccgaa
     4081 cccggacgcg gcggcccgca cgctggcgaa tcgccggatg gagacggccc gggcccagct
     4141 gcagaagacg gtgaagatcg cgccggagcg cttgcgcgtc accgagggcc tggtgccggt
     4201 ggagggctcc gggctcggac gcgtcgagtt cgagatcgcg ccctgagccc tagctgaaga
     4261 cgggccggcc tagctgaaga ccggattatc gacccggtcc accatcgagg ccggcccctc
     4321 ggtcggcagc acctcggcgt agttctgggc cgcgtcgatg tcgtgctccc agacctcgcg
     4381 cggcagctcg tagagcacct cgatcccgtg cccgtcgggg tcggagatgt acacgctgtg
     4441 ggtcatgccg tggttgatcc gccggtggaa ggtgacgccc ttctcgcgga ggtgctcgac
     4501 ctgcttgagc caggactcgc ggtccggcca ggcgatggcc acgtggttga gccccactcc
     4561 gccgcgcatc aggctccagg tctcgccctc gccctccgtc tcgccctcgc gcttcggggc
     4621 ctcggccagg gccaggtcgt ggtgggtgac gtcgccgctc tcggacacgc cgctgtagaa
     4681 gcgcatcttc ggccgcacgc ggtcgggccg cggcttgagc tgggccacgc agcggaagcc
     4741 catgatctcg gtccagaagc ggtgcgagac ctcgatgtcg cgcacgttga ggaccacgtg
     4801 gttgatgccg cggggcgtct gtgccgtcgc catggtgtcg tctccccttc gcctagaaca
     4861 tcccgttgcc gttggccgac ttctcgggaa tcggctggac cacgtcccag tgctccacga
     4921 tcttgccgtt ctccagcttg aagacgtcga cgatggccga gccgcgctcg cccggggtgc
     4981 ggaccgcgtg gacgtgcagg atcacgtagt ccccatccgc gagcacgcgc ttgatctc
//
LOCUS       DATMCV010000602         4124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_230072, whole genome shotgun sequence.
ACCESSION   DATMCV010000602 DATMCV010000000
VERSION     DATMCV010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230072"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VNU03_13905"
     CDS             complement(<1..594)
                     /locus_tag="VNU03_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263500066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(172..174),aa:Sec)
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWP75611.1"
                     /translation="MNATILHRNDAIRVGDASVEGEALWLTPEELARATEWEWTPDGL
                     CRGALCVPVPPGAPWVREAGPATRLDLAGLARHLGQPVAASPAHGVWAIGDSHAMLGE
                     RLDSLDAPDFTLPDLDGRAWSLRDFRGRKVFLLAWASWUGCRFDLPGWQALHAELEPK
                     GLTVITVALDSAGAAAAGEWIRAAQPTHPALIDAHYDL"
     gene            680..1009
                     /locus_tag="VNU03_13910"
     CDS             680..1009
                     /locus_tag="VNU03_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75612.1"
                     /translation="MVVTLAISAMVGLGHAPAPAADRSEVDAAAQQVQRGAGQIGQGK
                     VLEGAGETAKGIGNTVVEGAKYTGQKIGEAGQAAAPDAKSAWERTKEGAVAFGSSVRD
                     FFGSLFR"
     gene            complement(1034..3598)
                     /locus_tag="VNU03_13915"
     CDS             complement(1034..3598)
                     /locus_tag="VNU03_13915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HWP75613.1"
                     /translation="MDYRLPTTVVPSRYDIRLEPDLDAATFAGHETVAITVSEPVGEI
                     LLNAAELVIRSVSVQTADGQVVEGSASLDEAAERARLTFPQPIPPGEHRLTLDFTGIL
                     NDRLHGFYRSTYKDAAGASHTIAATQFEATDARRGFPCWDEPACKAVFGVTLVIPEAL
                     AAVSNTRVTGESPAGPGKKAVAFADTIRMSTYLVAFVVGELEATEPVMVGPTPLRVWC
                     VPGKLHLARFALEIGAFALDYFERYYGQPYPGDKLDMLAIPDFAAGAMENLGAITYRE
                     TALLVDEAAASHTERERVADVVAHEIAHMWFGDLVTMLWWNGIWLNEAFATFMELLAV
                     DAWKPEWKRWTTFGVSRAAAMAVDGLHNTRPIEFEVRAPRDCEAMFDLLTYEKGASVL
                     RMLEQHLSGELFRDGVRLYLERHRFGNAETTDLWKALGDAARLPIPEVMDGWIFRPGY
                     PLVIVEPDGTGIKLSQRRFTYLPLEGAASEHWQIPVRVRASVKRGFVDKPVLLGAEPL
                     TVSLPAAPDWVVVNAGGHGFYRVQYAAPLLRKMTSSLKKLAPIERFNLVSDAFALAQA
                     GLMPSTDYLDLTARFGEETDRNVWAAVTGSWGYVNRVIADDQRADLEALVRQRVQATV
                     ERLGWEAQPGETELERQLRADLLRTVGTLGNDPATQERARALYARYREDESAVDPDVL
                     PALIAIVAAAGGETEYDEFLQRFKAARSPQEEQRYLYALAAFRQPELIRRTLEKTTSG
                     EVRSQDAPFLMRALLAGVHSRGLAWDYVKANWETMARQYPGSAYRRMYEGVTALVSPE
                     WERDVREFFTSRKIVLGGKTLEQYLEQLRIAVSFQEREGAALAAYLAKPPRPPRAR"
     gene            complement(3672..>4124)
                     /locus_tag="VNU03_13920"
     CDS             complement(3672..>4124)
                     /locus_tag="VNU03_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75614.1"
                     /translation="LDDGSVRHTFPECFVCGHRRAPGDGLRIFAGPESGHGPRGRVRV
                     AGWTPDPTFLDEDGRLRPEFVWSALDCPGGWALAGPGNTGTLQVEIREPVDGRRPLIV
                     MGWRADPPTPRVGSRRRYAGTAMFDASGRLLALGAAIWVAPEPLATAR"
BASE COUNT          577 a         1487 c         1515 g          545 t
ORIGIN      
        1 caggtcatag tgcgcgtcga tcagggcggg atgggtcggc tgcgccgcgc ggatccactc
       61 ccccgccgcg gccgcgcccg cactgtcgag cgcgaccgtg atcacggtca gccccttcgg
      121 ctcgagctcg gcgtggagtg cctgccaccc gggcaggtcg aagcggcacc ctcaccacga
      181 ggcccacgcg agcaggaaca ccttgcggcc ccggaagtcc cgcagcgacc aggcccggcc
      241 gtccaggtcc ggcagcgtga agtcgggcgc gtcgagcgag tcgagccgct cgccgagcat
      301 cgcgtgcgag tcgccgatgg cccacacccc gtgcgccggg ctcgccgcga ccggctggcc
      361 gaggtgccgc gccaggccgg cgaggtcgag gcgcgtcgcg ggaccggcct cccgcaccca
      421 cggagcgccc ggcggcaccg ggacgcacag cgcgccccgg cagaggccgt ccggcgtcca
      481 ttcccattcc gtcgcgcggg ccagctcctc cggggtgagc cagagcgcct cgccctcgac
      541 ggaggcatcg cccacccgga tggcgtcatt gcggtgaaga atggtcgcgt tcatgcggtc
      601 cggccccggg cacggtgtta ccatgaattc gcgatcaagc caaccgaggg aggtgcgcac
      661 gatggtgaga gctcgctcga tggtggtcac gctggcgatc tcggcgatgg tcgggctggg
      721 tcacgccccc gcgccggcgg ccgaccgctc ggaagtggac gcggccgccc agcaggtgca
      781 gcgcggggcc ggtcagatcg gccagggtaa ggtgctcgag ggcgccggcg agacggcgaa
      841 gggcatcggc aacacggtgg tggaaggcgc caagtacacc ggccagaaga tcggggaggc
      901 cgggcaggcg gcggcgcccg acgccaagag cgcgtgggag cggaccaagg aaggcgcggt
      961 cgccttcggc tcgagcgtcc gggacttctt cggctccctc ttccgctgac cgcggcgcgg
     1021 gagcggccgg gccctagcgc gcccgcgggg ggcggggcgg cttggcgaga taggcggcga
     1081 gcgccgcgcc ctcgcgctcc tggaagctca ccgcgatccg gagctgctcc aggtactgct
     1141 cgagggtctt gccgccgagc acgatcttgc ggctggtgaa aaactccctc acatcgcgct
     1201 cccactccgg gctcaccagc gcggtcaccc cctcgtacat ccgccggtaa gcactccccg
     1261 gatactgccg ggccatcgtc tcccagttcg ccttgacgta gtcccaggcg aggccgcggc
     1321 tgtgcacgcc ggccaggagc gcccgcatca agaacggcgc gtcctggctg cgcacctccc
     1381 cgctggtggt cttctcgagg gtgcggcgga tcagctcggg ctggcggaac gcggcgagcg
     1441 cgtagaggta gcgctgctcc tcctgcgggc tgcgcgcggc cttgaagcgc tggaggaact
     1501 cgtcgtactc ggtctccccg cccgccgcgg ccacgatggc gatgagcgcg ggcagcacgt
     1561 ccgggtccac cgccgactcg tcctcccggt agcgggcgta gagggcgcgg gcgcgctcct
     1621 gggtggcggg gtcgttgccg agggtgccca cggtgcgcag gagatcggcg cgcagctgtc
     1681 gctccagctc ggtctcgccg ggctgggcct cccagccgag ccgctccacc gtcgcctgca
     1741 ctcgctggcg caccagggcc tcgagatcgg cccgctggtc gtccgcgatg acgcggttga
     1801 cgtagcccca cgagccggtg accgcggccc acacgttgcg gtcggtctcc tcgccgaagc
     1861 gcgcggtcag gtcgaggtag tccgtcgacg gcatcaggcc cgcctgggcc agcgcgaagg
     1921 cgtcgctcac caggttgaag cgctcgatgg gcgcgagctt cttgagcgag ctggtcatct
     1981 tgcgcaggag cggcgccgcg tactgcaccc ggtagaaccc gtgcccgccc gcgttgacca
     2041 ccacccagtc gggcgcggcg gggagcgaga cggtgagggg ctcggcgccc aggagcaccg
     2101 gcttgtccac gaagccgcgc ttgaccgagg cccgcacccg caccgggatc tgccagtgct
     2161 cggaggccgc gccctcgagc gggagatagg tgaagcggcg ctggctcagc ttgatcccgg
     2221 tgccgtcggg ctccacgatc acgagggggt accccggccg gaagatccag ccgtccatca
     2281 cctccgggat cggcagccgc gcggcgtcgc cgagagcctt ccagagatcg gtggtctccg
     2341 cgttgccgaa gcgatggcgc tcgagataga gtcgcactcc gtcgcggaat agctccccgc
     2401 tcagatgctg ctcgagcatg cgcaggaccg aggcgccctt ctcgtaggtg agcaggtcga
     2461 acatcgcctc gcagtcgcgc ggggcccgca cctcgaactc gatcggccgc gtattgtgca
     2521 gcccgtcgac cgccatcgcg gcggcgcgcg acaccccgaa ggtggtccac cgcttccact
     2581 ccggcttcca cgcgtcgacc gccagcagct ccatgaaggt ggcgaaggcc tcgttgagcc
     2641 agatgccgtt ccaccacagc atggtcacga ggtcgccgaa ccacatgtgg gcgatctcgt
     2701 gggccaccac gtcggcgacc cgctcgcgct cggtgtggga ggcggccgcc tcgtccacca
     2761 gcagcgcggt ctcccgatac gtgatcgcgc ccaggttctc catcgcgccc gccgcgaagt
     2821 cggggatcgc gagcatgtcg agcttgtcgc cgggatacgg ctggccgtag tagcgctcga
     2881 agtagtcgag ggcgaaggcg ccgatctcga gggcgaagcg ggccaggtgg agcttgcccg
     2941 gcacgcacca cacgcggagc ggggtgggac ccaccatcac cggctccgtg gcctcgagct
     3001 cgccgaccac gaaggccacc aggtaggtcg acatccggat ggtgtccgcg aaggccaccg
     3061 ccttcttgcc cggcccggcc ggggactcgc cggtcacccg ggtgttggac accgcggcca
     3121 gggcctccgg gatcaccagg gtgacgccga agaccgcctt gcacgccggc tcgtcccagc
     3181 acgggaagcc gcgccgcgcg tcggtggcct cgaactgggt ggccgcgatc gtgtgcgagg
     3241 cgcccgccgc gtccttgtag gtgctgcggt agaagccgtg gaggcgatcg ttgaggatcc
     3301 ccgtgaagtc gagcgtgagc cggtgctcac cgggcgggat cggctgcggg aacgtgagcc
     3361 gggcccgctc cgccgcctcg tcgaggctgg cgctcccctc caccacctgg ccgtcggcgg
     3421 tctgcacgct caccgagcgg atcaccagct ccgccgcgtt gagcaggatc tcgccgaccg
     3481 gctcggagac cgtgatcgcg acggtctcgt ggcccgcgaa ggtggcggcg tcgagatccg
     3541 gctcgaggcg gatgtcgtag cgcgagggca ccacggtggt cggcagtcgg tagtccatgc
     3601 cgggggaaag gataccatca gcccctcgcc cggacacccc gacttgtggc ccggagcgcg
     3661 gcgatccgcg gtcagcgcgc cgtcgcgagc ggctccggcg ctacccagat cgccgcgccg
     3721 agggcgagga gccgcccgct cgcgtcgaac atcgcggtgc ccgcgtagcg ccgccggctg
     3781 cccacgcgcg gagtgggcgg atccgcgcgc cagcccatca cgatcagggg ccggcgcccg
     3841 tccacgggct cgcggatctc cacctgcagg gtgccggtgt tgccggggcc ggccagcgcc
     3901 cagccgcccg ggcagtccag cgcggaccag acgaactcgg ggcggaggcg gccgtcctcg
     3961 tcgaggaacg tgggatccgg cgtccagccg gccacccgga cccggcccct ggggccgtgg
     4021 ccggactctg gccccgcgaa gatgcggaga ccgtcgccgg gcgcgcggcg gtgcccgcac
     4081 acgaagcact ccgggaaggt gtgccggacc gagccgtcgt cgag
//
LOCUS       DATMCV010000603         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_298977, whole genome shotgun sequence.
ACCESSION   DATMCV010000603 DATMCV010000000
VERSION     DATMCV010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298977"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VNU03_13925"
     CDS             complement(<1..828)
                     /locus_tag="VNU03_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75615.1"
                     /translation="MPAQPGGGETVLALDRVSKRFGEAAAVDSVSLSIRRGEIFTLLG
                     PSGCGKTTTLRLVAGLEQPDGGEITLRDRVVASAARRLFVAPNRRNLGMVFQSYAVWP
                     HMTVFENVAYPLEVRGLPRAAVREKVARILDLVGLGGTETRPGTLLSGGQMQRLALCR
                     ALVYEPDLLLLDEPFSNLDAKLREQMRVEVKLLQRRLGITVLFVTHDQVEALSLSDRI
                     AVMHRGRVEQVGTPRALYEQPTSAFVRDFLGQTVVLAGRVTASAAVRADACVTVAMNG
                     "
     gene            complement(849..1955)
                     /locus_tag="VNU03_13930"
     CDS             complement(849..1955)
                     /locus_tag="VNU03_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP75616.1"
                     /translation="MTLSRRRLLRTIAFGGAAAALTRATPARASVTPAEWQQVVAAAK
                     KEGKVVVNTFPGDGYKRALKAFSQAHPDIKLEHTGLHSQDFAPRVLQERQASLFTWDV
                     TTIPTSTALQVLRPAGVWDPVRPAIVLPEVLDDAGWDGGFERGFSAVKDRALAYAFCA
                     VRGAGVTINTDLVKDDQVKSLTDLLNPRWKGKLLLPDVRTMGDSFWSMTSARLNLGDD
                     IIKKLFVDQEPALSRDTRQIAEFMVRGRYPIALGVNPLLLSQFQKQGLGKNLKTLHFP
                     EMDTFNSSSSVVWLLNRAPHPSAAKVFINWLLTKDAQVAWAREVETNSRRLGVEPGNP
                     QYAVPRAAKLLQVDAEENLAEVVKTQDIGKAVIK"
BASE COUNT          294 a          737 c          775 g          323 t
ORIGIN      
        1 cccgttcatc gccaccgtga cgcaggcgtc ggcccgcacg gctgcggagg cggtgacgcg
       61 gcccgcgagc accaccgtct ggccgaggaa gtcacgcacg aaggcggacg tcggctgctc
      121 gtagagagcg cgcggcgtgc cgacctgctc cacgcgcccc cggtgcatga ccgcgatgcg
      181 gtcggagagg ctgagcgcct ccacctggtc gtgggtgacg aaaagcaccg tgatgccgag
      241 gcggcgctgc aggagcttca cctccacccg catctgctcg cgcagcttgg cgtccaggtt
      301 gctgaagggc tcgtcgagca gcaggagatc cggctcgtag acgagggcgc ggcagagggc
      361 caggcgctgc atctgcccgc cgctcagcaa ggtgccgggc cgggtctccg tcccgcccag
      421 gccgaccagg tccaggatcc gcgccacctt ctcgcgcacc gccgcccgcg ggagcccgcg
      481 cacctcgagg ggataggcca cgttctcgaa cacggtcatg tgcggccaga ccgcgtagga
      541 ctggaacacc atgccgaggt tgcggcggtt gggggcgacg aagagccgcc gcgccgcgga
      601 ggccaccacc cggtcgcgca gggtgatctc gccgccgtcg ggttgctcga ggccggccac
      661 cagccgcagg gtggtggtct tgccgcagcc gctcggcccg agcagggtga agatctcccc
      721 gcggcggatc gagagggaga cggagtccac cgccgccgcc tccccgaatc gcttgctgac
      781 gcggtcgagc gcgaggaccg tctcgccgcc gccgggctgc gcgggcactc tcggttccgt
      841 ggggtgcgtt acttgatgac cgccttgccg atgtcctgcg tcttgacgac ctcggccagg
      901 ttctcctccg cgtcgacctg caggagcttg gccgcccggg gaaccgcgta ctgcgggttg
      961 cccggctcga ccccgaggcg ccggctgttg gtctcgacct cgcgggccca cgccacctgg
     1021 gcgtccttgg tgagcagcca gttgatgaag accttggccg cgctcgggtg gggggcccgg
     1081 ttcagcagcc agaccacgct gctgctcgag ttgaaggtat ccatctcggg aaagtggagg
     1141 gtcttgaggt tcttgccgag cccctgcttc tggaactggc tcaggagcag cgggttgacc
     1201 ccgagcgcga tgggatagcg gccgcgcacc atgaactcgg cgatctggcg ggtgtcccgg
     1261 ctgagcgccg gctcctggtc cacgaagagc ttcttgatga tgtcgtcgcc caggttgagg
     1321 cgcgcgctcg tcatggacca gaacgaatcg cccatggtcc gcacgtcggg caggagcagc
     1381 ttgcccttcc agcgcgggtt caagagatcg gtcaggctct tgacctggtc gtccttcacg
     1441 aggtcggtat tgatcgtgac gcccgcgccg cggaccgcgc agaacgcgta ggccagcgcg
     1501 cggtccttca ccgcgctgaa gccgcgctcg aagccgccgt cccaccccgc gtcgtccagc
     1561 acctccggca gcacgatggc cggccgcacc ggatcccaca cccccgccgg ccgcagcacc
     1621 tggagcgcgg tgctcgtggg gatggtggtc acgtcccagg tgaagaggct cgcctggcgc
     1681 tcctgcagga cgcgcggggc gaagtcctgg gagtggagcc cggtgtgctc gagcttgatg
     1741 tcgggatggg cctggctgaa ggccttgagg gcccgcttgt agccgtcgcc ggggaaggtg
     1801 ttgacgacga ccttgccttc tttcttggcc gcggccacga cctgctgcca ctccgccggg
     1861 gtgacgctcg cgcgggccgg ggtggcccgc gtgagggccg ccgcggcgcc gccgaaggcg
     1921 atggtccgga gcagccgccg gcggctgagg gtcaccgggt ggcccgtcgc cgctgctcct
     1981 ggaggtagcg caccgagacc cggtcgagcg gcaccgcgtc cggcgagaga tcgtcggggt
     2041 tccggtactc gaacggcgcg tcgcggccgc cgaagtgctt ggtcaccacc aggcgacgcc
     2101 actggatcac ctccgcgtcg gtcgacgag
//
LOCUS       DATMCV010000604         4943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_366646, whole genome shotgun sequence.
ACCESSION   DATMCV010000604 DATMCV010000000
VERSION     DATMCV010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366646"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VNU03_13935"
     CDS             <1..641
                     /locus_tag="VNU03_13935"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75617.1"
                     /translation="YGLPGLDLATWTATVREVIAWGPDHLSAYGLTLDEGSLWHARGV
                     AGLPPEEAVTAQYWALAEAAAAAGFEHYEVSNYARPGFRSRHNQRYWRWEEYLGLGPG
                     ACGFLGHVRYANVKPVERYTTLVEAGQLPLATHEVLTPRQALAEQLILGLRTSDGIEA
                     TRLAERCDLERGRLPEVLDAWRERGLLADPEGRVRLTEAGFLLSDALFTELL"
     gene            662..1705
                     /gene="hrcA"
                     /locus_tag="VNU03_13940"
     CDS             662..1705
                     /gene="hrcA"
                     /locus_tag="VNU03_13940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWP75618.1"
                     /translation="MTTAAFDQRNRQVLLAVIAEYVETGEPVGSRAVARRHIQGLSPA
                     TIRNAMADLEELGYLVQPHTSAGRVPTDAAYRFYVDHLQRVPWATGKAPAVGAPVAPH
                     ADAVERLMAETPSRLSADTHMMGVLLAPPLKHTALDRIELVALDEERALAVLVTETGW
                     VTARPLTASPRGGGEELRETGRALTRRFRGKTFQEILDELAAPADPLDPLWTRSRAVL
                     EEVVSLLRDRTLYVSGATNMLDHPDLSDTATLRSMLRAFEDKVRLVDLLSRMAEERGL
                     HVVIGSENPVEDMRGCSLITSTYTYRDQVLGVLGVVGPRRMPYSEVMAVVDETARIVS
                     SSLSRVRQQLYLP"
     gene            1775..2314
                     /locus_tag="VNU03_13945"
     CDS             1775..2314
                     /locus_tag="VNU03_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWP75619.1"
                     /translation="MADSFFDGPIADDRAEEIQRLREALEAKTRELEVQQDRYLRTVA
                     EFDNVRKRAAREREEYTRYANESVLRDLLPVLDNLDRALLAARSEPAAAVTTGVELIQ
                     RELLRVLEKFGLTPFTSVGQPFDPGRHEAVARVQRPDLPEMTVADETGRGYLLNGRVL
                     RPAMVTVAMAPDQDHPADS"
     gene            2316..3407
                     /gene="dnaJ"
                     /locus_tag="VNU03_13950"
     CDS             2316..3407
                     /gene="dnaJ"
                     /locus_tag="VNU03_13950"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWP75620.1"
                     /translation="MAAKDYYGVLGVPRDADDAALKKAYRNLARAYHPDRNPGDKKSE
                     DRFKEIGEAYGVLSDPEKRAQYDRFGTVGGGMGEPGFGTIFEDLFEGFFGSGDRGRSR
                     TRARRGDDLRYDLEISLEEAALGLETKLQVPRLEACEPCRGSGVEPGSHPETCATCRG
                     QGQVRFSQGFLTVARPCPQCHGEGRIVRNPCTACRGQGRISRERLLKVTIPAGVEHGN
                     QMRLSGEGESGLLGGPPGDLYVVLHVRAHEIFVRDGAHLVCELPLTFAQVALGDEIEV
                     PVLDGAARLKIPPGTQPGQRLVLKGKGMPHLRGRGRGDAVYEVIVEVPTHLTARQREL
                     LEEFRGAHEGDSSPRVSKFLERMKQLFGS"
     gene            3407..4297
                     /locus_tag="VNU03_13955"
     CDS             3407..4297
                     /locus_tag="VNU03_13955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWP75621.1"
                     /translation="MAFWQLTVPSSAEISDGLTNFLWEQGALGVVEEEVPGVAPRLRA
                     FFAESMSSTGLLAAVNGYRASLHALGFALAGDAEIAPLLDEAWASAWQQSFPAREVGR
                     RLRVLPPWLDGPASGPAPSADDRITVVIEPGRAFGTGHHGTTEGCLVLLEEALAAAPG
                     AAVLDIGTGTGILAIAALRLGARAVLAIDLDPDAVAASRVNAERNRCAGLTVRLAEPQ
                     EVDEAAPIVVANLLTHSHLALAPHYARLVAPGGQLVLGGMLEDEDDRVGRALAASGFE
                     PRSRLALEGWASLRLAVRAR"
     gene            4294..>4943
                     /locus_tag="VNU03_13960"
     CDS             4294..>4943
                     /locus_tag="VNU03_13960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HWP75622.1"
                     /translation="MTRFHLDPGAVQGDRVTFAAPAAHHLGRVLRAAVGDLVQAVAAD
                     GALLSVRLTAIAPRRAEGHVVERTRLDTESPLRLTLAQAVPKGDKMEGIVRMTTELGV
                     SRLIPLLTERTVVRLPPDRWAARLARWQRVAREAAQQSGRAAVPEITAPRTLSTWRPD
                     PEPDGLLICFWEEEPGGIEGRLPAGPCSAATVIVGPEGGLTAEEVRGLAEAGALVAG"
BASE COUNT          641 a         1862 c         1781 g          659 t
ORIGIN      
        1 tctacggcct gcccggcctc gacctcgcga cgtggaccgc caccgtgcgc gaggtgatcg
       61 cgtggggccc ggaccacctg tccgcgtacg ggctcaccct ggacgagggc agcctctggc
      121 acgcgcgcgg cgtggcggga ctcccgcccg aggaggcggt caccgcgcag tactgggccc
      181 tcgcggaggc ggccgcggcg gccggcttcg agcactatga agtctccaac tacgcgcgtc
      241 ccggcttccg ctcgcgccac aaccagcgct actggcgatg ggaggagtac ctcgggctcg
      301 gaccgggcgc gtgcggcttc ctcggccacg tgcgctacgc caacgtcaag ccggtggagc
      361 gctacaccac cctcgtcgag gccgggcagc tcccgctcgc cactcacgag gtgctgaccc
      421 cgcgccaggc cctggccgag cagctcatcc tgggccttcg cacgagcgac gggatagaag
      481 ccacgcgcct cgcggagcgc tgcgatctcg agcgcggccg gctgcccgag gtgctcgacg
      541 cctggcgtga gcgcggcctg ctggccgatc ccgaaggccg cgtccggctc accgaggccg
      601 gcttcctgct ctccgacgcc ctgttcaccg agctgctcta gcggtaagat cgacggaccc
      661 catgaccacc gcggcgttcg atcaacggaa ccggcaggtc ctgcttgcgg tcatcgcgga
      721 gtacgtcgag accggggagc cggtcggctc gcgagcggtc gcgcgccgcc acatccaggg
      781 cctctcgccg gccaccatcc gcaacgcgat ggccgacctc gaggagctgg gctacctggt
      841 gcagccgcac acctcggccg gccgcgtgcc caccgacgcc gcctaccgct tctacgtcga
      901 ccacctccag cgcgtcccgt gggccaccgg caaggcccct gcggtgggcg cgcccgtggc
      961 cccccacgcc gacgcggtgg agcggctgat ggcggagacg ccgtcgcggc tctccgccga
     1021 cacccacatg atgggcgtgc tgctcgcgcc cccgctcaag cacaccgcgc tcgaccgcat
     1081 cgagctggtg gccctcgacg aggagcgggc cctcgcggtg ctcgtgaccg agaccggctg
     1141 ggtcaccgcg cgcccgctga ccgcgtcgcc ccggggcggc ggcgaggagc tgcgcgagac
     1201 cggccgcgcc ctcacccgcc gattccgcgg gaagaccttc caggagatcc tcgacgagct
     1261 ggccgcgccc gccgatccgc tcgatccgct gtggacgcgg agccgggcgg tgctcgagga
     1321 ggtcgtctcg ctgctgcgcg accggaccct ctacgtgagc ggcgccacca acatgctcga
     1381 ccacccggac ctgtcggaca ccgccaccct gcgctcgatg ctgcgggcct tcgaggacaa
     1441 ggtccgcctc gtcgacctgc tctcccgcat ggccgaggag cgcgggctgc acgtggtgat
     1501 cggcagcgag aacccggtgg aggacatgcg gggctgcagc ctgatcacct ccacctacac
     1561 ctatcgcgac caggtgctgg gcgtgctggg ggtggtgggg cctcgccgca tgccctactc
     1621 cgaggtcatg gcggtggtcg acgagacggc gcggatcgtc tcgagctcgc tctcccgggt
     1681 gcggcagcag ctctacctgc cgtagccgcc tccaccgggc cccggggcgc ctgcccagga
     1741 cccggcaatc cgtctgctat aattaccttc tgccatggct gattccttct tcgacggacc
     1801 gatcgcggac gacagggcgg aggagatcca gcgcctccgc gaggcgctgg aggccaagac
     1861 ccgcgagctc gaggtccagc aggaccgcta cctgcgcacg gtcgccgagt tcgacaacgt
     1921 gcgcaagcgc gcggcccggg agcgggagga gtacacccgc tacgccaacg agtcggtgct
     1981 gcgcgacctg ctgccggtgc tggacaacct cgaccgggcc ctgctggccg cccgctcgga
     2041 gccggccgcc gcggtcacga ccggcgtgga gctgatccag cgcgagctcc tgcgcgtgct
     2101 cgagaagttc ggcctgaccc cgttcacctc ggtgggccag cctttcgatc cgggccgtca
     2161 cgaggcggtg gcgcgggtgc agcgcccgga cctgcccgag atgacggtcg cggacgagac
     2221 cgggcgcggc tacctgctga acggtcgggt gctgcgaccc gcgatggtca cggtggcgat
     2281 ggcccccgac caggaccacc cggccgactc gtagcgtggc ggccaaggac tactacgggg
     2341 tcctcggggt cccgcgcgac gccgacgacg cggcgctgaa gaaggcctac cggaacctcg
     2401 cccgcgccta ccaccccgac cgcaatccgg gcgacaagaa gtccgaggac cgcttcaagg
     2461 agatcggcga ggcctacggc gtgctctccg atccggagaa gcgcgcgcag tacgaccgct
     2521 tcgggacggt cggaggcggg atgggcgaac ccggatttgg gaccatcttc gaggacctgt
     2581 tcgaggggtt cttcggcagc ggagatcgcg ggcgctcgcg cacccgcgcg cggcggggcg
     2641 acgaccttcg ctacgatctg gagatctcgc tcgaggaggc ggccctgggc ctcgagacca
     2701 agctccaggt gccgcggctc gaggcctgcg agccctgccg cggctcgggc gtcgagccgg
     2761 gcagccaccc ggagacctgc gccacctgcc gcggtcaggg ccaggtgcgc ttctcgcagg
     2821 gcttcctcac ggtggcccgt ccgtgtcccc agtgccacgg cgaggggcgc atcgtccgca
     2881 acccctgcac cgcctgccgg ggccagggcc gcatctcccg cgagcgcctg ctcaaggtca
     2941 ccatcccggc cggagtcgag cacggcaacc agatgcggct cagcggcgag ggcgagagcg
     3001 ggctcctcgg ggggcctccc ggcgatctct acgtggtgct ccacgtgcgc gcgcacgaga
     3061 tcttcgtgcg cgacggcgcg cacctggtct gcgagctacc gctcaccttc gcccaggtgg
     3121 cgctcggcga cgagatcgag gtgccggtgc tggacggcgc cgcccggctc aagatccctc
     3181 ccggaaccca gccgggccag cggctggtgc tcaagggcaa gggcatgccg cacctccgcg
     3241 gccggggccg gggcgacgcg gtctacgagg tcatcgtcga ggtgccgacc cacctgaccg
     3301 cgcgccagcg ggagctgctg gaggagttcc ggggcgccca cgagggcgac tcgagcccgc
     3361 gggtctccaa gttcctcgag cggatgaagc agctgttcgg cagttgatgg cgttctggca
     3421 gctcaccgtc ccctcctcgg ccgagatctc cgacgggctg accaacttcc tgtgggagca
     3481 gggcgccctc ggcgtcgtcg aggaggaggt tccgggcgtt gccccgcggc tgcgcgcctt
     3541 cttcgccgag tcgatgtcgt ccaccgggct cctggccgcg gtgaacggct accgggcctc
     3601 gctgcacgcg ctcggcttcg ccctggccgg tgacgccgag atcgcgcccc tgctcgacga
     3661 ggcgtgggcg agcgcctggc agcagtcctt ccccgctcgc gaggtcggcc gccggctgcg
     3721 cgtgctgcca ccctggctcg acggcccggc ctccggaccg gcgccgagcg cggacgaccg
     3781 gatcacggtc gtgatcgagc cggggcgcgc gttcgggacc ggtcaccacg gcaccaccga
     3841 gggctgcctc gtgctcctgg aggaagccct cgccgccgca cccggcgccg cggtcctcga
     3901 catcggcacc ggtaccggca tcctggccat cgcggcgctc cggctcggcg cccgcgccgt
     3961 gctcgcgatc gacctcgatc cggacgcggt ggcggcgagc cgggtcaacg cggagcggaa
     4021 ccgctgcgcc ggcctcacgg tgcggctcgc cgagccccag gaggtcgacg aggcggcgcc
     4081 gatcgtggtg gcgaacctgc tcacccacag ccacctggct ctcgcgcccc actacgcgcg
     4141 cctcgtggcg ccgggcgggc agctcgtgct gggcggcatg ctcgaggacg aggacgaccg
     4201 ggtcggccgc gcgctcgccg catccggctt cgagcctcgc tcgcgcctgg ctctcgaggg
     4261 ctgggcctcg ctgcgcctgg ccgtgcgcgc ccggtgaccc gcttccacct ggatccgggt
     4321 gcggtgcagg gcgaccgggt gacgttcgcc gcccccgccg cgcaccacct gggccgggtg
     4381 ctacgcgccg cggtgggcga tctggtgcag gcggtggccg ccgacggagc gctcctctcg
     4441 gtgcgcctca ccgccatcgc gccccggcgc gcggagggcc acgtcgtcga gcgcacccgg
     4501 ctcgacaccg agtcgcccct tcgcctgacg ctggcccagg ccgttcccaa gggcgacaag
     4561 atggagggca tcgtccgcat gaccaccgag ctcggggtga gccggctgat tcccctgctc
     4621 accgagcgca ccgtcgtgcg cctgcccccc gaccgctggg ccgcgcggct cgcccgctgg
     4681 cagcgcgtgg cgcgcgaggc cgcccagcag agcggccggg ccgcggtgcc cgagatcacg
     4741 gcgccgcgca cgctctcgac ctggcggccc gatccggagc ctgacggcct cctgatctgc
     4801 ttctgggagg aagagcccgg cgggatcgag gggcggcttc ccgccgggcc gtgctcggca
     4861 gcgacggtga tcgtgggacc ggagggcgga ctcaccgccg aggaggtgcg cggcctcgcc
     4921 gaggccgggg cgctggtggc cgg
//
LOCUS       DATMCV010000605         2777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_167048, whole genome shotgun sequence.
ACCESSION   DATMCV010000605 DATMCV010000000
VERSION     DATMCV010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167048"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VNU03_13965"
     CDS             complement(<1..1081)
                     /locus_tag="VNU03_13965"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP75623.1"
                     /translation="MSRLAASKRARLPATAFAYIDSRGRRRLPIHDEAHVRNALARFS
                     RVAFENDADRERTRTRLLNAAKKYGIVPVGFITGQLQSERSNATAGRLVIELGRHGAP
                     GELEQRLRSVLRDPTLAVLHWSEASGAYLDGAGRPAPLPAEGDRRGVTFLERRGRPMT
                     ALVHDPTVLDDPGVAETVLAAVRFVVEKELLHGQIQATSTDAGALPTGFVTLLMTDIE
                     RSTGLLRRLGDRYGDLLNDVRGILRTAVLRAGGREIDARADEYFAVFEQATAAVAAAV
                     AVQRAVGKRGWPDDLDVRVRAGIHSGRPTLTEVGYIGLAVHTTARVCSAAHGGQIVVS
                     SRTRAAVRTATRAGVSFRGLGRHRLP"
     gene            complement(1095..2150)
                     /locus_tag="VNU03_13970"
     CDS             complement(1095..2150)
                     /locus_tag="VNU03_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWP75624.1"
                     /translation="MRASRLLAWGGVVALAWLARGLLLPVFLAAALAYLLSPAVAWAD
                     ALAIRRSVAVGALFTAIVTVLVVVGFLLGPGMVAETAALVDRLPTLARQIDVGLNAAV
                     RELAESAPALRRLLPEGGDWVQRFVLNRGPGEPADLFEHVGHLFLLVILVPFFAFFLL
                     RDMHRLIALAMDRLPPQHVETSVAVWRELNGVIGRYIRGVVLDGLVVGALAALGLWAA
                     RVPYPLLLGAFAGLANVVPFVGPLLSAAAAGLVVVLTPGQGLAGVVRVVMLFLVIKVL
                     DDTIIQPFTIGRSVHLHPALLLGSVVVGNHALGVLGMIIAVPAATVLQETVRLLLEHR
                     RVLARRDLRGRPERLVA"
     gene            complement(2147..2671)
                     /locus_tag="VNU03_13975"
     CDS             complement(2147..2671)
                     /locus_tag="VNU03_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWP75625.1"
                     /translation="MFEQPLPRWLAWATDPEQVRRAPFLSRSPLFTGLPRRLLARLST
                     RFFEKVYHPGEVVFEEGDPGRALFVVVDGAVEITRATPQGSYVLNTLGPGDAFGELAL
                     IDDFPRSATARVVTPGRLLILYKSDFDALMEGNARIAVVMMRNLSRRLAIYARRQPGA
                     MSEPGPGSETAGPL"
BASE COUNT          442 a         1029 c          992 g          314 t
ORIGIN      
        1 cgggcagacg gtgccgcccg agcccgcgga agctcacgcc ggcccgcgtc gcggtccgga
       61 ccgcggcccg ggtgcggctc gacaccacga tctgcccgcc gtgcgccgcc gagcagacgc
      121 gcgcggtggt gtgaacggcc agcccgatgt acccgacctc ggtcagcgtc gggcgtccgc
      181 tgtgaatccc cgcgcgaacc cggacgtcga gatcgtcggg ccacccgcgc ttgccgacgg
      241 cacgctggac cgccaccgcc gccgcgaccg cggcggtcgc ctgctcgaag accgcgaagt
      301 actcgtccgc gcgggcgtcg atctcccggc cgcccgctcg cagcaccgcg gtgcggagaa
      361 tgccccggac gtcgttcaac aggtcgccgt agcgatcgcc cagccgtcgt aggaggccgg
      421 tggaccgctc gatgtcggtc atcaagaggg tgacgaaccc ggtgggaagc gctccggcat
      481 cggtcgacgt ggcctggatc tggccgtgca ggagctcctt ctcgaccacg aagcgcaccg
      541 cggccagcac ggtctcggcc acacccgggt cgtcgaggac cgtcggatcg tgcacgaggg
      601 ccgtcatcgg ccgaccccgg cgctcgagga aggtcacgcc ccgccggtct ccctcggcgg
      661 gaagcggggc gggcctgccg gcgccgtcca ggtacgcgcc ggaggcctcg gaccagtgca
      721 ggaccgcgag ggtcggatcg cgcaggacgc tccgcaggcg ctgctcgagc tcaccgggcg
      781 ccccgtggcg gcccagctcg atcacgagtc gcccggccgt cgcgttgctg cgctccgact
      841 gcagctgtcc ggtgatgaag ccgaccggga cgatgccgta cttcttcgcc gcgttcagga
      901 ggcgggtgcg cgtgcgctcc cggtcggcgt cgttctcgaa ggcgacccgg ctgaaccggg
      961 cgagggcgtt ccgcacgtgg gcctcgtcgt ggatcggcag ccgccgccgg ccgcgcgagt
     1021 cgatgtaggc gaaagcggtg gccggcagcc gggcccgttt actggccgcg aggcgagaca
     1081 tgcgtcagga ccggtcaggc gaccagccgc tccggtcgcc cgcgcaggtc gcgtcgggcc
     1141 agcacccggc ggtgctcgag gaggagacgc accgtctcct ggagcaccgt ggccgccggc
     1201 accgcgatga tcatgccgag caccccgagc gcgtggttgc cgaccaccac cgagccgagc
     1261 agcagcgcgg ggtggaggtg aacgctccgc ccgatggtga agggctggat gatcgtgtcg
     1321 tcgagcacct tgatgacgag gaacagcatc accacgcgca cgaccccggc cagcccctgc
     1381 cccggcgtca gcaccaccac gagcccggcg gccgcggccg agagcagcgg ccccacgaac
     1441 ggcaccacgt tggcgaggcc ggcaaacgcg ccgagcagga gcggatacgg aacgcgcgcc
     1501 gcccacaggc ccagggcggc cagcgcgccc accacgaggc cgtccagcac caccccgcgg
     1561 atgtagcgcc cgatgactcc gttcagctcg cgccacaccg ccaccgacgt ctccacgtgc
     1621 tgcggaggca gccggtccat cgccagggcg atgagacggt gcatgtcccg caacaggaag
     1681 aaggcgaaga acggcacgag gatcaccagc aggaagaggt gccccacgtg ctcgaagaga
     1741 tcggccggct cgccggggcc ccggttgaga acgaatcgct gcacccagtc cccgccctcc
     1801 ggcaagaggc gccggagcgc gggcgccgac tcggccagct cgcgaaccgc cgcgttcagg
     1861 ccgacgtcga tctgccgcgc cagcgtgggc aggcgatcca ccagcgccgc ggtctccgcg
     1921 accatacccg gcccgagcag gaacccgacg accaccagca ccgtgacgat ggccgtgaac
     1981 agcgcgccca ccgccacgct ccgccggatg gccagggcat ccgcccacgc gaccgccggg
     2041 ctcaggaggt aggcgagcgc ggcggcgagg aacacgggca ggagcaggcc gcgcgccagc
     2101 caggccagtg cgacgacccc gccccacgcg agcagccggc tcgctctcac agcggacccg
     2161 ccgtctccga ccccggaccc ggctcgctca tggccccggg ctgacggcgc gcgtagatgg
     2221 cgagcctccg ggagaggttc cgcatcatca cgaccgcgat ccgggcgttg ccttccatga
     2281 gcgcgtcgaa gtcgctcttg tagaggatga gcagccgccc cggcgtgacg actcgcgcgg
     2341 tggccgagcg cgggaagtcg tcgatgaggg cgagctcgcc gaaggcatcg ccggggccga
     2401 gggtgttcag gacgtacgag ccctgcggcg tcgcccgggt gatctccacc gcgccgtcga
     2461 ccacgacgaa caacgcgcgg ccgggatcgc cctcctcgaa gacgacctcg cccggatggt
     2521 agaccttctc gaagaaccgc gtcgacagcc gggccagcag gcggcgtggc aggccggtga
     2581 agagcggaga gcgcgacagg aagggcgccc ggcggacctg ctcggggtcc gtcgcccagg
     2641 ccagccaccg tggcaggggc tgctcgaaca ttgcgcgcac tatagcagaa ggccatgcgc
     2701 atggcgcgca gctccaccgg acgaccctct cttgccgcgc tatccctgcc cgcgctatcc
     2761 ctgaatgagg atgagat
//
LOCUS       DATMCV010000606         5853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_829397, whole genome shotgun sequence.
ACCESSION   DATMCV010000606 DATMCV010000000
VERSION     DATMCV010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829397"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VNU03_13980"
     CDS             complement(<1..228)
                     /locus_tag="VNU03_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HWP75626.1"
                     /translation="MTELRAEGLRSRTLIFAACLACAFVVLLGRLAYLQILKHDEYSR
                     LAENQHAKTVPLRPKRGPILDRGGQALAVSSR"
     gene            complement(238..699)
                     /locus_tag="VNU03_13985"
     CDS             complement(238..699)
                     /locus_tag="VNU03_13985"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HWP75627.1"
                     /translation="MSSRVATSRAAQSHWGHQHRARQPEAVRRHRVSDRRRLRSMFTA
                     VVGAGLAVVLVLGVVGLRVQQVRLSYRLDALRQGRAELEETNRRLRVEMATLKSLSRI
                     EGKARGELGMVAPGRDQVRLAREFVQGGGGAAALDRRAELGGGASRSGGVQ"
     gene            complement(696..1610)
                     /gene="rsmH"
                     /locus_tag="VNU03_13990"
     CDS             complement(696..1610)
                     /gene="rsmH"
                     /locus_tag="VNU03_13990"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWP75628.1"
                     /translation="MREHAEAEHAHVPVLADEVTFLLRPRRGGWVVDGTIGMGGHAEQ
                     LLEHAGGDTRLLGIDRDPEALARAARRLARFGARVVLCHGSFRHLGALATGAGVERAA
                     AVLLDLGLSSYQLDESGRGFSFQDAEPLDMRFDPTEGATAAELLGRLSAEEIASLLGE
                     LGEERHARRIARVLVEQRRRAPLSTAADLVSVVKRAVPRAAWSKRTHVATRTFQAVRM
                     AVNDEPGALAEALPQAAALLETGGRLGVISFHSGEDRVVKRAFRSLAGRGFVELEPTP
                     VTAGREEMAFNPRARSAKLRVLARQEAA"
     gene            complement(1784..2224)
                     /gene="mraZ"
                     /locus_tag="VNU03_13995"
     CDS             complement(1784..2224)
                     /gene="mraZ"
                     /locus_tag="VNU03_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWP75629.1"
                     /translation="MFRGHFRHTIDPKGRLSIPAKFREVLREAGSDRLVMAPNGTAID
                     VYPEDKWRELEDRVNGLAKLDPDRRRFQHVYLSAGLDVVLDPHGRIQILQDYRERGGL
                     SKNVVIIGMMDHFEIWDDDRWSIYQREQAGPLDDLRERLAQKGV"
     gene            complement(2451..3230)
                     /locus_tag="VNU03_14000"
     CDS             complement(2451..3230)
                     /locus_tag="VNU03_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWP75630.1"
                     /translation="MPRRFRALALLLIALTLTPLGCETVPYTGRRQLQLVSPDEEAKM
                     GVQSYQEIVGKAPLSTDGQSSAMVQRVGSRIAAVTDLKYQWEFKLIADDKQANAFALP
                     GGKVAVYTGILPITRDEAGLAAVLGHEIGHVLARHGGERLSQQMGVQSATQVLAGLAS
                     SNPATVQLVGAALGAGAQVGVLLPWSRQQESEADHLGLILMAKAGYDPHAALELWKRM
                     AEAAKGQRPSQWLSTHPSEETRIEQIQGWLPEALTYYRPTK"
     gene            complement(3240..3782)
                     /locus_tag="VNU03_14005"
     CDS             complement(3240..3782)
                     /locus_tag="VNU03_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75631.1"
                     /translation="MRHAPAGYSLVDLLVALAVGGGLMASTLTLLHLGLRAWVWGAAR
                     VDAQQSARYAVERMAGELREAGYDPTVAGIAPVVLAEPARVVFQRDFNGNGVVDPTRE
                     RVTYLLRPGETILRRDAGGGAQPVVEQVRAFRLTYLDRAGAETTDAAAVTAVRIRLEV
                     GETGSAVIVETQATLRNLLW"
     gene            complement(3867..5282)
                     /locus_tag="VNU03_14010"
     CDS             complement(3867..5282)
                     /locus_tag="VNU03_14010"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP75632.1"
                     /translation="MANADDLCWMPASEMAPAIKRRKISPVEVMKAVLARIERLNPRL
                     NAFVTLTPEQAMKTARAAERAVGKKGARLGALHGVPFSTKDLVITKGIRTTFGTRLFA
                     DNVPTESAPMVERLEAAGAIQLGKTNTPMMGWIGATHNLLFGITRNPWNLDRTPGGSS
                     GGASAAVAAGLGPLAIGTDGGGSIRIPASLAGIYGIKPTFGLVPIHPFSAAWGLSHIG
                     PMTRTVADAALMLDACAGPDARDPLSLPGPRVDYVKALKGGVKGLRVAWTTDLGFAKV
                     VDPEVASACERAARRFRELGCRVEEVAPKWPSPHAAWRTMFLGGIAARLGPALRDRRE
                     DIDPGLVAIADETRGWSPTQYVQSWFDRLAWEEHPRRLFERYDLLLTPTIACAAFKVG
                     QDGPPEIAGTPTGIYDWIPFTYPFNMTGHPGASVPCGFTREELPIGLQIVGRRFEDAT
                     VLRASAAFEKLAPWAQRRPLD"
     gene            complement(5335..>5853)
                     /locus_tag="VNU03_14015"
     CDS             complement(5335..>5853)
                     /locus_tag="VNU03_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75633.1"
                     /translation="DLVTQRVMDILQGLPLLVLALVMSAALGPSIPNVVIAISIPIIP
                     RAARVIRASVLSIREMQYVEAARAVGLRHLRIAFRHILPNTIGPFIVLCTAQLGSAIL
                     VEAALSFLGLGIPEPYPSWGRMLSVSAAEYAQKAPWLVLFPGLAISLAVFGSNLLGDA
                     LRDTLDPRLRGA"
BASE COUNT          822 a         2098 c         2124 g          809 t
ORIGIN      
        1 gcgggaggag accgcgaggg cctgcccgcc gcggtccagg atcggcccgc gcttgggacg
       61 gagcggcacc gtcttggcgt gctgattctc ggccagccgc gagtactcgt cgtgcttgag
      121 gatctgcaga tacgccagcc ggccgagcag gacgacgaag gcacaggcca ggcacgccgc
      181 gaagatcagc gtccggcttc ggaggccctc ggcccgcagc tcggtcatgg agtcacgcta
      241 ttggaccccg ccgctccggg aagctccgcc gccgagctcc gcgcgccggt cgagcgccgc
      301 ggcgccgccg ccgccctgga cgaactcgcg cgcgaggcgc acctggtcgc ggcccggggc
      361 gaccatcccg agctcgccgc gggccttgcc ctcgatgcgg gagagcgact tcagggtggc
      421 catctcgacc cgcagccgcc ggttcgtctc ctccagctcg gcgcgcccct gccggagcgc
      481 gtcgaggcgg taggacaggc gcacctgctg cacccgcagc cccaccactc ccagcaccag
      541 gaccaccgcg agcccggccc ccaccaccgc ggtgaacatg gagcggagcc ggcgccgatc
      601 gctgacccgg tgccgccgga ccgcttccgg ctggcgggcg cgatgctggt gaccccagtg
      661 cgactgtgct gcgcgtgacg tggcgacccg actgctcatg ctgcctcctg ccgcgcgagc
      721 acgcgcagct tggcgctgcg ggcgcgcggg ttgaacgcca tctcctcgcg gcccgcggtc
      781 accggagtcg gctcgagctc gacgaagccc cggcccgcca gtgagcggaa cgcgcgcttg
      841 accacccggt cctcaccgga atggaaggag atgacgccga gccgtccgcc ggtctcgagg
      901 agcgcggccg cctgcggcag cgcttcggcg agggcgccgg gctcgtcgtt gacggccatc
      961 cgcaccgcct ggaaggtgcg agtggccacg tgcgtgcgct tggaccaggc cgcgcgcggg
     1021 accgcgcgct tcaccaccga gaccaggtcg gcggcggtgc tgaggggcgc gcgccgccgc
     1081 tgctcgacga gcacgcgcgc gatgcgccgc gcgtgccgct cctcgcccag ctcgccgagg
     1141 aggctcgcga tctcctccgc ggagagccgg ccgagcagct ccgccgcggt cgccccctcg
     1201 gtgggatcga atcgcatgtc gagcggctcc gcgtcctgga acgagaaccc gcgccccgac
     1261 tcgtcgagct ggtaggacga gagcccgagg tcgagtagca ccgcggcggc ccgctcgacg
     1321 cccgcgccgg tggcgagcgc gccgaggtgg cggaagctgc cgtgacagag caccacgcgc
     1381 gcgccgaagc gcgcgaggcg ccgcgcggca cgcgcgagcg cctccggatc gcgatcgatg
     1441 ccgagcaggc gcgtgtcgcc gcccgcgtgc tcgagcagct gctcggcgtg gccgcccatt
     1501 ccgatcgtgc cgtccaccac ccagccgccg cggcgcggac gcagaaggaa ggtcacctcg
     1561 tccgcgagga ccgggacgtg ggcgtgttcg gcttctgcgt gctcgcgcat ccgtgttgcg
     1621 tgttcgggct cgttccggct tacaccggaa gcttctctat tgaatgcgct cggctcgcct
     1681 tcggctgcgc ctcgccctgc cactgctcgg ctgccgaacc gtgcgcgtga tccgcggtcg
     1741 ggccgtggct gggctgcgcg gtcgttgcgt ttgcggcgca tggctagacg cccttctgcg
     1801 cgaggcgctc gcgcaggtcg tcgagcgggc cggcctgctc gcgctggtag atcgaccagc
     1861 gatcgtcgtc ccagatctcg aagtgatcca tcatcccgat gatcaccacg ttcttcgaga
     1921 gaccgccgcg ctcgcgatag tcctgcagga tctggatgcg cccgtgcgga tcgagcacca
     1981 cgtcgagacc ggccgagaga tacacgtgct ggaatcgacg gcgatccgga tcgagcttgg
     2041 cgagaccgtt cacgcggtcc tcgagctcgc gccacttgtc ctcggggtac acgtcgatcg
     2101 cggtgccgtt gggcgccatc acgagccgat cactgccggc ctcgcgcagc acctcgcgga
     2161 acttcgccgg gatgctgagt cgccctttcg ggtcgatcgt gtgcctgaaa tgtccccgaa
     2221 acactgtctc ccccgccggg gcaccaaact cgcccctgga ccggccctga atgacacttc
     2281 atgggatttc aacccacttc gagggaccat cctagggggg atcgattggg ggtgtcaagc
     2341 gaaaactccg gattttcaag ccattccgtc ccgccggaaa ggccaccggt tgggggtggc
     2401 ccgggcaggc ctacagcagg tagacggagg gcctcgaggg acgggcctgg ctacttggtg
     2461 ggacggtagt acgtcagggc ctcgggcagc cagccctgga tctgctcgat gcgggtctct
     2521 tcggaggggt gcgtcgacag ccactgggag ggccgctggc ccttggcggc ctcggccatg
     2581 cgcttccaca gctcgagcgc cgcgtgggga tcgtagcccg ccttcgccat gaggatcagg
     2641 cccagatgat cggcctccga ctcctgctga cggctccacg gcagcagcac gcccacctgc
     2701 gctcccgcgc cgagggcggc gcccaccagc tggacggtgg ccgggttgct gctggccagg
     2761 ccggccagca cctgcgtggc gctctgcacg cccatctgct ggctcagccg ctcgcccccg
     2821 tgccgcgcga gcacgtggcc gatctcgtgg ccgagcaccg cggccaggcc ggcctcgtcg
     2881 cgggtgatgg ggaggatacc ggtgtagacc gccacctttc cccccggcag cgcgaaggcg
     2941 ttggcctgct tgtcgtcggc gatgagcttg aactcccact ggtacttgag gtcggtcacc
     3001 gccgcgatgc ggctacccac gcgctggacc atcgcgctgg attgaccgtc ggtggagagc
     3061 ggggccttgc ccacgatctc ctggtaggac tgcacgccca tcttcgcttc ctcgtcgggc
     3121 gagacgagct gaagctggcg gcggcccgta tacggcaccg tctcgcagcc gagcggcgtc
     3181 agggtgagcg cgatcagcag cagcgcgaga gcacggaatc ggcgaggcac gcgcgcattc
     3241 taccagagca ggttgcggag cgtggcctgg gtctccacga tgacggcgga tcccgtctcg
     3301 ccgacctcga gccggatgcg caccgcggtc accgcggccg catcggtcgt ctccgcgccg
     3361 gcgcggtcga gataggtgag ccggaaggcc cgcacctgct cgaccacggg ctgtgcgccc
     3421 ccacccgcgt cgcggcgtag gatcgtctct cccggccgca ggagataggt gacgcgctcg
     3481 cgggtcgggt ccaccacccc gttgccgttg aagtcccgct ggaagacgac gcgggccggc
     3541 tcggccagca ccaccggcgc gatgcccgcc acggtggggt cgtagccggc ctcgcgcagc
     3601 tcgccggcca tgcgctcgac cgcgtagcgg gcggactgct gggcgtcgac gcgcgcggcc
     3661 ccccagaccc aggcgcgcag ccccaggtgc aggagcgtga gggtcgaggc catgagcccg
     3721 ccgcccaccg ccagggcgac gaggagatcg accagcgagt agccggcggg ggcgtggcgc
     3781 accccgcgag cgtgtccggg gccgccggcc cccgcaatgc ccggctttcg agacgcgccc
     3841 gggaagagac gtgtatcgtc gggcgctcag tcgaggggcc gccgctgcgc ccagggcgcg
     3901 agcttctcga aggcggcgga ggcgcgcagc acggtggcgt cctcgaagcg gcggcccacg
     3961 atctggagcc cgatgggcag ctcctcgcgg gtgaagccgc acggcaccga ggcgccgggg
     4021 tgcccggtca tgttgaacgg ataggtgaac gggatccagt cgtagatccc ggtcggggtg
     4081 cccgcgatct cgggcggccc gtcctgcccc accttgaagg ccgcgcacgc gatggtcggg
     4141 gtgagcagca ggtcgtagcg ctcgaagaga cgccgcgggt gctcctccca ggcgagccgg
     4201 tcgaaccagg actggacgta ctgcgtggga ctccagccgc gggtctcgtc cgcgatggcc
     4261 acgaggcccg gatcgatgtc ctcccgccgg tcgcgcaggg ccgggcccag ccgggccgcg
     4321 atgccgccca ggaacatggt ccgccacgcg gcgtgcgggc tcggccactt cggggcgacc
     4381 tcctcgacgc gacagcccag ctcgcggaag cgccgggccg cgcgctcgca cgcgctcgcc
     4441 acctcgggat ccaccacctt ggcgaagccg agatcggtgg tccacgccac ccgcagaccc
     4501 ttcacgccgc ccttgagcgc cttcacgtag tccacccggg gccccggcag cgacagggga
     4561 tcgcgcgcgt cgggccccgc gcaggcgtcg agcatgagcg cggcgtccgc caccgtgcgg
     4621 gtcatcgggc cgatgtgcga gagcccccag gccgcgctga acgggtggat cgggacgagc
     4681 ccgaaggtcg gcttgatgcc gtagatgccc gcgagcgacg ccgggatgcg gatcgagccg
     4741 ccgccgtcgg tgccgatcgc gagcggcccc aggccggcgg ccaccgcggc gctggcgccc
     4801 ccgctcgagc cgcccggggt gcggtcgagg ttccacgggt tgcgggtgat gccgaagagc
     4861 aggttgtggg tggcgccgat ccagcccatc atcggggtgt tggtcttgcc gagctggatc
     4921 gcccccgccg cctcgagccg ctcgaccatc ggcgcgctct cggtcggcac gttgtcggcg
     4981 aagagccgcg tgccgaacgt ggtgcggatg cccttggtga tcacgagatc cttggtggag
     5041 aacggcaccc cgtgcagcgc gccgagccgc gcccctttct ttcccaccgc gcgctcggcc
     5101 gcgcgcgccg tcttcatcgc ctgctcgggg gtcagggtga cgaaggcgtt caggcgcggg
     5161 ttcaggcgct cgatgcgggc gagcaccgcc ttcatgacct cgaccggcga gatcttgcgg
     5221 cgcttgatcg cgggggccat ctcggacgcg ggcatccagc agaggtcgtc ggcattggcc
     5281 atggagaact ccccctcacc ctgccctctc ccccgagggg agagggatgt gtggttaggc
     5341 gccgcggagg cgcggatcga gcgtgtcgcg gagcgcgtcg ccgagcaggt tggagccgaa
     5401 gacagccagg ctgatggcca ggcccgggaa gagaacgagc cacggcgcct tctgggcgta
     5461 ctccgcggcg gagaccgaga gcatccggcc ccacgaggga tagggctccg ggatcccgag
     5521 accgaggaac gagagcgcgg cctccaccag gatcgcgctg ccgagctggg cagtgcacag
     5581 cacgatgaag gggccgatgg tgttgggcag gatgtggcgg aacgcgatgc gcaggtggcg
     5641 cagccccacc gcgcgcgccg cctccacgta ctgcatctcg cggatcgaga gcacgctggc
     5701 ccggatgacc cgcgcggccc gcgggatgat cgggatcgag atcgcgatca ccacgttcgg
     5761 gatcgacggc ccgagcgccg ccgacatcac gagggccagc accagcagcg gcaacccctg
     5821 caggatgtcc atcacccgct gggtgacgag gtc
//
LOCUS       DATMCV010000607         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_695022, whole genome shotgun sequence.
ACCESSION   DATMCV010000607 DATMCV010000000
VERSION     DATMCV010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695022"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VNU03_14020"
     CDS             <1..331
                     /locus_tag="VNU03_14020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWP75634.1"
                     /translation="ASRSRLLDMGLVDPEHAEIAARIRQALAPTAAEDLKPRARKIQQ
                     FFAQPFYVAEPYTHQPGVTVSRAEALQICRDTLDGVHDRLPEAAFYFTGGMDEIRAKV
                     EVEGSKP"
     gene            328..732
                     /locus_tag="VNU03_14025"
     CDS             328..732
                     /locus_tag="VNU03_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HWP75635.1"
                     /translation="MTEQKPPGTSWESWIEQQIREAREAGLFDNLPGAGKPLANLTDE
                     YDPLWWVKQLAQREQISLLPPSLEVLRKVESEMAAIWQLADEAKVRSRILALNREIAK
                     VNARSTEGPPTRLGPLDVEAIVADWRARRASS"
     gene            complement(734..2107)
                     /locus_tag="VNU03_14030"
     CDS             complement(734..2107)
                     /locus_tag="VNU03_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWP75636.1"
                     /translation="MHPRPPEFPTTLQPTAPRTAELDRLGDQIAELAAHLDAATARLL
                     ALIREFDDRGGWNTGFRSCAEWLTWRIGLAPGSARERVRVARALGSLPAVAAALERGQ
                     VSYAKVRALTRVATPETEARLLAVARAGTAVHVERIVQGWRQVDRQNEARKAARQHAA
                     RSLRIYPDADRRLVLRGRLAPEVGALLLRALDAARETLYQRRRAGAIIPSGVDPAAAA
                     PTRTQQQADALALLAEAALRHELDPGSPGERYQVVVHVDAAVLADPARAGESALEDGP
                     RVPAGTSQRLACDASRVVMRHDADGRVVEVGARTRTIPPALRRALHHRDRSCRFPGCT
                     VRVGEGHHVRHWAQGGPTTLSNLALLSRRHHRSVHEEGYQMERGSDGALQFRRPDGRP
                     LPGVPAPPPLPADPVEALRAEHTAQGLQIHARTGIAGWLGERLDVGWAIDVLHPLATR
                     AIVRRAT"
     gene            complement(2346..3251)
                     /locus_tag="VNU03_14035"
     CDS             complement(2346..3251)
                     /locus_tag="VNU03_14035"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWP75637.1"
                     /translation="MQPVTTVSAGQAPRRLDAFVSTQTAHLSRAVVQRWIDGGLITVN
                     GQKTKPARLIRPGDVIACHVPVREPRAVEPEPLALRVLHEDSALLVIDKPPGLVMHPG
                     PGHWTGTLLNGLMHHVDPGAGRRARPGLVHRLDKGTSGVLVIAKTDAAHRHLSRQFRA
                     HSVHRVYLALVAGAVRRGGLIDQGLGRDTRDRRRVSVRSAAPRRAVTEYRVAERLGPH
                     ETLVEARPQTGRMHQIRVHLASIGHPVLGDASYGAAPPDPALSRPMLHAAVLGFVHPV
                     SGEYVEFRAPLPADMERAVADHRAS"
BASE COUNT          482 a         1177 c         1170 g          435 t
ORIGIN      
        1 cgcctcccgg tcccgcctcc tggacatggg cctcgtggat ccggagcacg cggagatcgc
       61 cgcgcggatc cggcaggcgc tcgcgcccac ggcggcggag gatctgaaac cgcgcgcccg
      121 caagatccag cagttcttcg cccagccgtt ctacgtggcc gagccgtaca cgcaccagcc
      181 cggcgtcacg gtcagccggg ccgaggccct ccagatctgc cgcgacaccc tcgacggcgt
      241 gcacgatcgg ctccccgaag cggcgttcta cttcacgggc ggaatggacg agatccgtgc
      301 gaaggtggag gtcgagggat cgaagccatg accgagcaga agccgccggg aacgagctgg
      361 gagtcctgga tcgagcagca gatccgggag gcgcgcgagg ccggcctctt cgacaacctg
      421 ccgggcgccg ggaagccgct ggcgaacctg acggacgaat atgatcctct ctggtgggtc
      481 aagcagctcg cgcagcgtga gcagatctcc ctgctgccgc cgtccctcga ggtgctccgc
      541 aaggtcgaat ccgagatggc ggcgatctgg cagctcgccg acgaggccaa ggtcaggtct
      601 cgaatcctgg cgctgaatcg agagatcgcc aaggtgaacg cgcggtcgac cgagggcccg
      661 ccgacccgcc tcggccccct cgacgtggag gcgatcgtcg cggactggcg cgcccggcgc
      721 gcgtcctcgt agctcaagtc gctcgtcgga cgatggctcg cgtcgccagc gggtggagca
      781 cgtcgatcgc ccatcctacg tccaggcgct ccccgagcca tcccgcgatt ccggtgcgcg
      841 catggatctg aaggccctgc gcggtgtgct ccgcccgtag cgcctccacc ggatcagcgg
      901 gcaatggcgg cggcgcgggc accccgggca gcggccgacc atccggccgt cggaactgga
      961 gcgcgccgtc ggacccgcgc tccatctggt agccctcctc gtgaaccgag cggtgatggc
     1021 gacggctcag tagcgccaga ttcgacagcg tggtcgggcc tccctgggcc cagtggcgca
     1081 cgtgatgccc ctcacccact cgaacggtgc agcccgggaa ccggcagctc cggtcccgat
     1141 ggtgcagagc ccgccggagc gccggcggga tcgtccgcgt gcgggctccg acttccacca
     1201 cccgcccgtc cgcgtcgtgc cgcatgacca cccggctcgc atcgcaggcc agccgttgcg
     1261 acgttcccgc gggaacgcgc ggcccgtctt cgagcgccga ctccccggcc cgagccggat
     1321 ccgccaggac cgcggcgtct acatggacca cgacctgata ccgctccccg ggcgagccgg
     1381 ggtcgagctc gtgacgaagc gctgcctcgg ccagcaaggc cagagcatcc gcctgctgct
     1441 gcgtccgggt cggagcggcc gcggcgggat cgacacccga gggaatgatt gcgccggcac
     1501 ggcgccgctg gtagagggtc tcgcgggccg cgtcgagcgc acgcagcagc agcgcgccca
     1561 cttccggggc aaggcgcccg cgcagcacga gccttcggtc cgcatccggg tagatgcgga
     1621 gagagcgagc ggcatgctgg cgggcagcct tgcgagcctc gttctgccgg tctacctggc
     1681 gccacccctg cacgatgcgc tccacgtgaa ccgcggtgcc cgcgcgcgct accgcgagca
     1741 atcgggcctc ggtctccggg gttgccacgc gggtcagagc acgcaccttg gcgtaggaca
     1801 cctgtccccg ctccagggcc gccgcaacgg ccggcagcga cccgagagcg cgtgctaccc
     1861 gaacgcgctc acgagcagag cccggggcga gcccgatacg ccacgtgagc cactcggcgc
     1921 aggagcgaaa gcccgtgttc cagccgccgc ggtcgtcgaa ctcgcggatg agcgcaagga
     1981 gtcgggcggt cgccgcatcg aggtgggcgg cgagctcggc gatctggtcg cccaggcggt
     2041 ccagctccgc ggtgcgcggt gccgtgggct gcagcgtggt gggaaactcc ggtgggcgcg
     2101 ggtgcatgat gagtcctccc ttctacttgg agaactctaa cccctggatt tcggcccgcc
     2161 ccagatcgcg tacgtcacgc gatcgccgcg tacgtcacca tccgtcgtct ctgccctcgt
     2221 gaacgtgaat cacgcggccg atgcggagca ctccgccgtc atttcagcag cctgctcgac
     2281 ccacgccgca gccgtcgcaa aaagctgtca agaggaaatc gtcactcgcc gcgcgccgat
     2341 cgccctcagc tcgcgcgatg gtcggcgacc gcccgctcca tgtcggccgg caggggcgcg
     2401 cggaactcca cgtactcgcc gctcaccggg tgcacgaagc cgagcacggc ggcgtggagc
     2461 atgggacggc tcagcgcagg atcgggcggc gccgcgccgt agctcgcgtc gcccaggacc
     2521 gggtggccga tcgaggccag gtgcacgcgg atctgatgca tgcggccggt ttgcgggcgc
     2581 gcctcgacca gggtttcgtg ggggccgagc cgctcggcca cgcgatactc cgtcaccgcc
     2641 cgtcgcgggg ccgccgagcg aaccgagacg cggcgtcgat cgcgggtgtc acgccccagt
     2701 ccctggtcga tcagcccgcc ccgccggacc gcgcccgcca ccagggcgag atagacgcgg
     2761 tggacggagt gcgcccggaa ctggcgcgag agatggcgat gggccgcgtc ggtcttcgcg
     2821 atcacgagga cgccgctcgt gcccttgtcg aggcgatgca ccagcccggg tcgggcgcgc
     2881 cgcccggcgc cggggtcgac gtggtgcatc aagccgttga gcaaggtgcc cgtccagtgg
     2941 ccggggccgg ggtgcatcac gagacccggg ggcttgtcga tcaccaggag cgccgaatcc
     3001 tcgtgcagga cgcgcagcgc gaggggctcg ggctcgaccg cccggggctc gcgcacgggg
     3061 acgtgacagg cgatcacgtc gcccgggcga atcagccgcg cgggcttggt cttctggccg
     3121 ttgacggtga tcagcccgcc gtcgatccac cgctggacga ccgcgcgcga gaggtgcgcg
     3181 gtctgggtgg acacgaaggc gtcgaggcgt cgcggggcct gcccggcgga caccgtcgtc
     3241 accggctgca tcgcggccga tcag
//
LOCUS       DATMCV010000608         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_167158, whole genome shotgun sequence.
ACCESSION   DATMCV010000608 DATMCV010000000
VERSION     DATMCV010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167158"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VNU03_14040"
     CDS             <1..595
                     /locus_tag="VNU03_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWP75638.1"
                     /translation="AHAPGVEKKYVEDGKMSNRRLVMYNDFVLIGPTDDPAQIKGLPK
                     AVDALKRIAESQSRFVSRGDKSGTHVLEQGLWKQAGLEPRGAWYIESGQGMGQTLGIA
                     NDRRAYTLTDRGTYLAFQKRVDLPILVEKDRPLLNIYSVMEVNPANGPRVNVAGGKAF
                     AEFMLAPETQAVIKAFGVDKYGQPLFVPVAGKKDEDL"
     gene            614..1309
                     /locus_tag="VNU03_14045"
     CDS             614..1309
                     /locus_tag="VNU03_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75639.1"
                     /translation="MGLFGQGVLEALRLLVTGDPEVWQITWLSLQVSATATLLSLLVG
                     IPVGALLALSRFPGRAFVVSLVNTGMGLPPVVVGLFVTILLWRNGVLGFLELLYTPTA
                     MVTGQLVIAAPIVTGLTLAAVQQVPEAFRLQLLGLGASRPQMIWVLLKEARLPMLAAV
                     MAGFGGVISEVGASMMLGGNIKGSTRVLTTATVLETGKGNFDVAIALSVILLLITFLV
                     NWALSVIQQRRRG"
     gene            1382..1918
                     /locus_tag="VNU03_14050"
     CDS             1382..1918
                     /locus_tag="VNU03_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75640.1"
                     /translation="MRMLIGGLIILSFAGSGVAENVSSPPRDEVIRGLTEEEISALQQ
                     GLGMGRARAAELNGYPGPRHVLDLAQARQLALAPEQVRTAQRLFDDMAREAQRLGDLI
                     LREERTLEAEFRAGGISETNLHTRALRIAALEGELRAVHLRAHIEMRAALSGPEIQQY
                     NALRGYVEAPEHQHKPHH"
BASE COUNT          292 a          681 c          706 g          322 t
ORIGIN      
        1 ggcccacgcc ccgggggtcg agaagaagta cgtcgaggac ggcaagatga gcaaccggcg
       61 cctggtcatg tacaacgact tcgtgctcat cggccccacc gacgatcccg cgcagatcaa
      121 gggcctgccg aaggcggtcg acgcgctcaa gcgcatcgcg gagagccagt cgcgcttcgt
      181 ctcccgcggc gacaagtccg gcacccacgt gctcgagcag gggctgtgga agcaggccgg
      241 cctcgagccc cggggcgcct ggtacatcga atccgggcag ggtatgggcc agactctcgg
      301 catcgccaac gaccggcgtg cctacaccct gaccgaccgc gggacgtacc tcgcgttcca
      361 gaaacgggtg gacctgccga tcctcgtcga gaaggaccgc ccgctgctca acatctactc
      421 ggtgatggag gtgaacccgg ccaacggccc gcgggtgaac gtggccggcg gcaaggcgtt
      481 cgccgagttc atgctcgcgc cggagaccca ggcggtgatc aaggcgttcg gggtggacaa
      541 gtacggccag ccgctcttcg tgccggtggc cggcaagaag gacgaggacc tctagggcgg
      601 ggcccgccgc gccgtgggcc tgttcgggca gggcgtcctc gaagcgctcc ggctcctcgt
      661 caccggggat ccggaggtct ggcagatcac ctggctctcc ctccaggtct ccgccaccgc
      721 caccctgctc tcgctgctgg tcggcatccc ggtgggcgcg ctgctggcgc tctcccgctt
      781 tcccggccgc gccttcgtgg tgagcctggt gaacaccggc atgggcctgc ccccggtggt
      841 ggtgggcctc ttcgtcacga tcctgctctg gcgcaacggc gtgctcggct tcctcgagct
      901 gctctacacg cccaccgcga tggtgaccgg ccagctcgtg atcgcggcgc cgatcgtgac
      961 cgggctcacc ctggccgcgg tgcagcaggt accggaggcc tttcgcctgc agctgctggg
     1021 gctcggcgcc tcgcggccgc agatgatctg ggtgctcctc aaggaggcgc ggctgccgat
     1081 gctggccgcg gtgatggcgg gcttcggcgg cgtgatctcc gaggtgggcg cgtcgatgat
     1141 gctgggcggc aacatcaagg gatccacccg cgtgctcacc accgccaccg tgctcgagac
     1201 cggcaagggc aacttcgacg tggcgatcgc gctctcggtg atcctgctgc tgatcacctt
     1261 cctggtgaac tgggcgctga gcgtgatcca gcagcgccgg cgggggtagg gactggtgta
     1321 aggttgcccc gcgggcccgc ccggcgtccc aggggcgggg ccacgggaaa aggagccgac
     1381 ggtgcgcatg ctgattggcg ggctgatcat cctgtcgttt gcggggtccg gcgtcgcgga
     1441 gaatgtgtcg tccccgcccc gggatgaggt cattcgaggc ttgaccgagg aggaaatctc
     1501 cgcgctccag caggggctcg ggatggggcg tgcccgggcc gcggagctga acggctatcc
     1561 gggaccccgc catgtcctgg acctcgccca ggcccgtcag cttgctctcg ctcccgagca
     1621 ggtgcggacc gcgcagcgtc tgttcgacga tatggcgcgg gaggcccagc gcctgggcga
     1681 tctgatcctc cgagaggagc ggaccctgga ggccgagttt cgtgccggtg ggatcagcga
     1741 gacgaacctg cacactcgcg ctctgcggat tgccgcgctc gaaggggagc tacgggcagt
     1801 acatctgcgc gcccacatcg agatgcgcgc ggccctctcg gggccggaaa tccagcagta
     1861 caacgctctc cgcggctacg tcgaagctcc cgagcaccag cacaagccgc accactgatc
     1921 ccgtctcgcg cgtcaccacc cgggctagcg cttgatcact cgtgccgggg tttggccttt
     1981 cttccgatgg gctccgggtg g
//
LOCUS       DATMCV010000609         3599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_299616, whole genome shotgun sequence.
ACCESSION   DATMCV010000609 DATMCV010000000
VERSION     DATMCV010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299616"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VNU03_14055"
     CDS             <1..658
                     /locus_tag="VNU03_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75641.1"
                     /translation="SNGRVDCGLGAGWHDVEAKAFGYPFPSIGPREDMLEEYAQIMRL
                     LLDPERRRVTFEGKHFRMVDAPNNPKPLQPRIPIWIGGRGEKRTLRAAAKFADGWNGA
                     YIGPDDWVHKSQVLDRWCETEGRDPKTIMRTVNLGFYLGADTRGAARGEEVFRRHWGG
                     TPDRADRSGYLRGTVKDARTMVDQFRALGCTRLCIAFREGPYDWDALEAFAAEIVRAR
                     "
     gene            673..1107
                     /locus_tag="VNU03_14060"
     CDS             673..1107
                     /locus_tag="VNU03_14060"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:802..804,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWP75642.1"
                     /translation="MLNPGDKAPDFTGRDHNGQTVKLADLKGKTVVLWFYPKADTPGU
                     TAEGCGFRDLKADYDRKNAVILGVSFDTPAQNRAFAERFHFNFPLICDTDRAIGTAYG
                     ANADPAKGAQRVGVVIGPDGKIKEWHARVDARAWPAAVVGTL"
     gene            complement(1120..3048)
                     /locus_tag="VNU03_14065"
     CDS             complement(1120..3048)
                     /locus_tag="VNU03_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HWP75643.1"
                     /translation="MALSATEAPPSTHPLDPLSAAEIERAWEIVRGERALGPRVRVIF
                     VMLHEPAKKVVLEHRPGDPVDRAAFVVLIDSAAGKTYEAIVSLAEGRVLSWGHIPGAQ
                     PAIVLDEFTDCEAAVRADPRWQEAVRKRGVTDPSLAMVDCWSAGNFGFAADEGRRLVR
                     ALTWVRRHPGDNGYARPIANLLTVVDLNEMKVVAVEDGGVVPLPPEDASYSAEAAGTR
                     TGLKPIEIRQPEGPSFTLAGHELSWQKWRMRIGFTPREGLVIHTVSYADQGRERPVLY
                     RASIADMVVPYGDPRPTYFHRNAFDVGEYGIGTLANALVNGCDCLGEIAYLDAVVNDG
                     RGHAVTLPNAVCIHEEDAGILWKHVDWRRGSTEVRRSRRLVVSSISTVGNYEYGFYWY
                     FYQDGTIQLEVKLTGIISNGAEPAGSRPRWGEMVAPGVYGPIHQHFFNARLDMMVDGL
                     ENSVYEVNTVADPPGPENPHANAFHAESTLLRSEGQAQRVLDPLAGRFWKIANPLVRN
                     RLGEPVAYKLMPGENVLPFAGPDASVTKRAGFMTRHLWVTRHDPRERYAAGEYPNQHA
                     GGAGLPAYVADDAPLESTDLVVWYTFGAHHVVRPEDWPVMPVVTIGFMLKPTGFFDRN
                     PGLDVPPSTPNGSCCHSG"
     gene            complement(3104..3466)
                     /locus_tag="VNU03_14070"
     CDS             complement(3104..3466)
                     /locus_tag="VNU03_14070"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWP75644.1"
                     /translation="MRFLLLMYREERIWDAKPEAERVAIRQQAIDASRPLRERGVFLA
                     GDPLLPTSTAVTVRIAGGKPVATDGPFAETKEQLGGYSVVDVPSIKEAVAIALDHPLV
                     RVGGHSIEVRPIRELPQR"
BASE COUNT          531 a         1321 c         1254 g          493 t
ORIGIN      
        1 ctcgaacggc cgcgtcgact gcggcctcgg cgccggctgg cacgacgtgg aggcgaaggc
       61 cttcggctac ccgttcccct cgatcgggcc gcgcgaggac atgctcgagg agtacgcgca
      121 gatcatgcgg ctgctgctcg accccgagcg ccggcgcgtc acgttcgagg ggaagcactt
      181 ccgcatggtc gacgccccca acaacccgaa gccgctccag ccccgcatcc cgatctggat
      241 cggcggccgc ggcgagaagc gcaccctgcg cgcggccgcc aagttcgccg acggctggaa
      301 cggcgcctac atcggcccgg acgactgggt ccacaagagc caggtcctcg accgctggtg
      361 cgagacggaa gggcgcgacc cgaagacgat catgcgcacc gtgaacctcg gcttctactt
      421 gggcgccgac acgaggggcg ccgcgcgggg cgaggaggtc tttcgccgcc actggggcgg
      481 tacccccgac cgcgccgacc ggagcggcta cctccgcggc accgtgaaag acgcgcggac
      541 gatggtggac cagttccgcg cgctcggctg cacccggctc tgcatcgcat tccgcgaggg
      601 gccgtacgac tgggacgcgc tcgaggcctt cgcggccgag atcgtgcgcg cccgctgacg
      661 agaggagagc tcatgctcaa tcccggagac aaggcgcccg acttcacggg gcgcgaccac
      721 aacggacaga ccgtgaagct ggcggacctc aagggcaaga cggtcgtgct ctggttctac
      781 cccaaggccg acacccccgg ctgaaccgcc gaaggttgcg ggttccgcga cctgaaagcc
      841 gactacgacc gcaagaacgc cgtgatcctc ggcgtgtcct tcgacacccc ggcccagaac
      901 cgggccttcg ccgagaggtt ccacttcaat ttccccctga tctgcgacac cgaccgcgcc
      961 atcggcaccg cctacggcgc caacgccgac ccggccaagg gcgcgcagcg cgtgggggtc
     1021 gtcatcggtc ccgacggcaa gatcaaggag tggcatgcgc gcgtcgacgc gcgggcctgg
     1081 ccggccgccg tggtcggcac gctctagcgg gcgccgcgct cagcccgagt ggcagcagct
     1141 cccgttcggg gtcgacgggg gcacgtcgag gccggggttg cggtcgaaga atccggtcgg
     1201 cttgagcatg aagccgatgg tgaccaccgg catcaccggc cagtcctcgg ggcgcaccac
     1261 gtgatgagcc ccgaaggtgt accagaccac caagtcggtg ctctcgagcg gtgcgtcgtc
     1321 ggccacgtag gcgggcagac ccgcgccgcc tgcgtgctgg ttcgggtact cgccggccgc
     1381 gtaccgctcg cgcgggtcgt ggcgcgtcac ccagagatgg cgcgtcatga accccgcgcg
     1441 cttcgtcacc gacgcgtccg ggcccgcgaa cggcagcacg ttctcgcccg gcatcagctt
     1501 gtaggcgacc ggctccccca gccggttgcg caccagcgga ttggcaatct tccagaagcg
     1561 gcccgcgagc ggatcgagga cccgctgcgc ctggccctcc gatcgcagca gggtcgactc
     1621 ggcgtggaac gcgttggcgt gcgggttctc gggccccggc gggtcggcca ccgtgttcac
     1681 ctcgtagacc gagttctcga gcccgtccac catcatgtcg aggcgcgcgt tgaagaagtg
     1741 ctggtggatc ggcccgtaga ccccgggggc gaccatctcg ccccaccgcg gcctggagcc
     1801 cgccggctcg gccccgttcg agatgatccc ggtgagcttg acctcgagct ggatggtccc
     1861 gtcctggtag aagtaccagt agaagccgta ctcgtagttg ccgacggtgg agatggacga
     1921 caccacgaga cggcgcgagc gccgcacctc cgtgctcccc cgccgccagt cgacgtgctt
     1981 ccagaggatg cccgcgtcct cctcgtggat gcacaccgcg ttgggcaggg tcaccgcgtg
     2041 gccccggccg tcgttgacca ccgcgtcgag ataggcgatc tcgcccaggc agtcgcagcc
     2101 gttgaccagc gcgttggcca gcgtgccgat gccgtactcg cccacgtcga aggcgttgcg
     2161 atggaagtag gtcgggcgcg gatcgccgta gggcaccacc atgtccgcga tcgaggcccg
     2221 gtagaggacc gggcgctcac gcccctggtc cgcgtaggac acggtgtgga tcaccagccc
     2281 ctcgcgcggc gtgaacccga tccgcatccg ccacttctgc cagctgagct cgtggcccgc
     2341 gagggtgaag ctcgggccct cgggctgccg gatctcgatg ggcttgaggc cggtgcgcgt
     2401 cccggccgcc tccgccgagt agctggcgtc ctccggcggc agcgggacga cgcccccgtc
     2461 ctcgaccgcg acgaccttca tctcgttgag atcgacgacg gtgagcaggt tggcgatcgg
     2521 gcgcgcgtag ccgttgtccc ccggatggcg ccgcacccag gtgagcgcgc ggacgagccg
     2581 gcgcccctcg tcggccgcga agccgaagtt gcccgccgac cagcagtcca ccatggcgag
     2641 gctcgggtcg gtgaccccgc gcttgcgcac ggcctcctgc cagcgcgggt cggcgcgcac
     2701 cgccgcctcg cagtcggtga actcgtcgag gacgatggcg ggctgggccc ccgggatgtg
     2761 cccccacgag agcacgcgcc cctcggcgag cgagacgatc gcctcgtagg tcttgcccgc
     2821 cgcggagtcg atcagcacca cgaaggcggc ccgatccacc ggatcgccgg gccggtgctc
     2881 gagcacgacc ttcttggccg gctcgtggag catcacgaag atcacccgga cccgcggccc
     2941 gagcgcgcgc tcgccgcgca cgatctccca ggcgcgctcg atctccgccg cgctcagggg
     3001 gtccagcgga tgggtactcg ggggtgcctc ggtcgcgctc agggccatgg ctcgtctcct
     3061 ctgccgcgat tgtagcggag ccggcctccc cccgcgaccc cgcctaccgc tgcggcagct
     3121 cccggatggg ccgcacctcg atcgagtggc cgcccacccg caccagcgga tgatcgagcg
     3181 cgatcgcgac cgcctccttg atgctcggga cgtccaccac ggagtagccg cccagctgct
     3241 ccttggtctc ggcgaacggg ccgtccgtcg cgaccggctt gcctcccgcg atgcgcaccg
     3301 tgaccgcggt gctggtcggc agcagcggat cgcccgcgag gaagaccccg cgctcgcgga
     3361 ggggccgcga cgcgtcgatg gcctgctgac ggatcgcgac ccgctccgcc tcgggcttgg
     3421 cgtcccagat ccgttcctcg cggtacatca gcagcagaaa acgcatcgcg gggtgccgcg
     3481 ccctcagcgg gccgggctcg ggctcggcac gccgacggcg acgcgccccg gcgggcggaa
     3541 cgtggacgcg acggccaccg cggccaggcc gatggcgaac gagctcaggt agagccagg
//
LOCUS       DATMCV010000610         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_230898, whole genome shotgun sequence.
ACCESSION   DATMCV010000610 DATMCV010000000
VERSION     DATMCV010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230898"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VNU03_14075"
     CDS             <1..357
                     /locus_tag="VNU03_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75645.1"
                     /translation="ELTGLAGRANTRAGTLTLLERKRLELARALATAPRVLLLDEIAG
                     GLSEPEVHQLVEVIQRVHQEGVAIVWIEHIVHALRSVVSRLAVISAGRLLTEGPPDDV
                     MRSPEVQRVYLGIDVQ"
     gene            354..1052
                     /locus_tag="VNU03_14080"
     CDS             354..1052
                     /locus_tag="VNU03_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75646.1"
                     /translation="MSLLQVQRLSAFYGSFQALFDVSLEAAEGETIAVIGANGAGKTT
                     CLRAIAGVLPVPVEAVRFAGDAVGGRPAHDLVARGVVMVPEGRRIFPSLTVEENLFVG
                     AYRGRPGPWTLARVYDLFPVLAERRRQPGPNLSGGEQQMLAIGRGLMGNPRLLLVDEI
                     SLGLAPIVVRGLYRVLRTIAAEGTTILVVDQDVSQIMDLAQRVYCFRKGAVSLEGRPA
                     DLTRARLAAAYFGV"
     gene            1055..1927
                     /locus_tag="VNU03_14085"
     CDS             1055..1927
                     /locus_tag="VNU03_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003554581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75647.1"
                     /translation="MAWVDTILQGLLLGGLYALFATGLSLIFGVMRIVNLAHGDLSIL
                     AAFLAVVAVESLGLDPFVALVLVAPLMAAFGYVLQTLVLNRLLGQGILPPILVTFGLS
                     IIIQNALLEIFSADSRRLDPGGIETASVALGGQLAVGWLPLITLAVAVAILLGLQGLI
                     GRLSVGRAFRATSDDQETAELMGIDNRRLYGLAMALSLGIVAVAGVFLGIRTTFTFAS
                     GPDRLLFAFEAVIIGGLGSLWGTLVGGAVLGVAQTVGAKLSPGWGILTGHLVFLAVLL
                     LRPQGLFAKARRVA"
     gene            2023..>2339
                     /locus_tag="VNU03_14090"
     CDS             2023..>2339
                     /locus_tag="VNU03_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75648.1"
                     /translation="MSLPAWGDASMMRILVEFIALLGLAQMWNLLAGYAGLVSIGQQA
                     YVGLGGYALIVLADDLGVDPFVAVPLAGLVTAALAPTAALVFRFRGGYFAVGTWAIAE
                     VYR"
BASE COUNT          247 a          848 c          858 g          386 t
ORIGIN      
        1 gagctgaccg gcctcgcggg gcgggccaac acccgcgcgg gcaccctgac cctgctggag
       61 cgcaagcgtc tcgagctggc ccgcgcgctc gccaccgcgc cccgggtgct gctgctcgac
      121 gagatcgcgg gcggcctctc cgagccggag gtgcaccagc tcgtcgaggt gatccagcgc
      181 gtccaccagg agggggtggc gatcgtgtgg atcgagcaca tcgtccacgc gctccgctcg
      241 gtggtgagcc ggctcgcggt catcagcgcg gggcgcctgc tgaccgaggg cccgcccgac
      301 gacgtgatgc ggagcccgga ggtccagcgc gtctacctgg gcatcgacgt gcagtgagcc
      361 tcctccaggt ccagcgcctg agcgccttct acggcagctt ccaggccctc ttcgacgtct
      421 cgctggaggc cgcggagggc gagaccatcg cggtcatcgg cgccaacggg gcggggaaga
      481 ccacgtgcct ccgggcgatc gcgggcgtgc tgccggtgcc ggtggaggcg gtgcgcttcg
      541 cgggcgacgc ggtgggtggg cgtcccgcgc acgacctggt ggcccgcggg gtggtgatgg
      601 tgccggaggg ccggcgcatc ttcccgagcc tgaccgtcga ggagaacctg ttcgtcggcg
      661 cttatcgcgg ccgccccggc ccgtggaccc tcgcgcgcgt ctacgatctc ttcccggtcc
      721 tggccgagcg ccgccgtcag cccggtccga acctctcggg gggcgagcag cagatgctcg
      781 cgatcggacg ggggctcatg ggcaatcccc ggctgctcct ggtcgacgag atctcgctgg
      841 gcttggcccc gatcgtcgtg cgcggactct accgggtgct gcgcaccatc gcggccgagg
      901 gcaccaccat cctcgtggtc gaccaggacg tgagccagat catggacctg gcccagcgcg
      961 tctactgctt ccgcaagggc gcggtgtcgc tcgaggggcg gccggccgac ctcacccgcg
     1021 cccggctcgc cgccgcgtac ttcggggtgt gacggtggcc tgggtcgaca ccatcctcca
     1081 ggggctgctc ctgggcgggc tctacgcgct cttcgcgacc ggcctctcgc tcatcttcgg
     1141 cgtcatgcgc atcgtgaacc tggcccacgg cgatctcagc atcctggccg cctttctcgc
     1201 ggtggtcgcg gtggagtcgc tcggcctcga cccgttcgtg gccctggtcc tggtcgcgcc
     1261 gctgatggcc gcgttcggct acgtgctgca gacgctggtc ctgaaccggc tgctcgggca
     1321 ggggatcctg ccgccgatcc tggtgacgtt cgggctctcg atcatcatcc agaacgcgct
     1381 cctcgagatc ttctcggccg actcccgccg gctcgacccg ggcggcatcg agaccgcgag
     1441 cgtggccctc ggcgggcagc tcgcggtggg ctggctcccg ctcatcacgc tcgcggtggc
     1501 ggtggcgatc ctcctcgggc tgcaggggct gattggccgg ctatcggtgg gccgcgcctt
     1561 ccgcgcgacc tcggacgacc aggagaccgc ggagctgatg ggcatcgaca accgccggct
     1621 ctacggcctc gccatggcgc tctccctcgg catcgtggcg gtggcggggg tgttcctcgg
     1681 catccgcacc accttcacct tcgcgagcgg cccggaccgg ctgctcttcg cgttcgaggc
     1741 ggtgatcatc ggagggctcg gctcgctctg gggcacgctg gtgggcggcg cggtgctcgg
     1801 cgtcgcgcag accgtcgggg ccaagctgtc gccgggctgg ggcatcctga ccgggcacct
     1861 ggtcttcctc gcggtgctgc tcctccggcc ccagggcctg ttcgcgaagg cccggagagt
     1921 ggcctgagcc cgcccgcgcg ctccgggacc gtccgcgtcg agcgcaccac gcgggccggc
     1981 cgcgccggcc tggtcggcgc cgcgctgggg ctggtcgccc tggtgagcct gcccgcgtgg
     2041 ggggacgcga gcatgatgcg gatcctggtg gagttcatcg ccctgctcgg cctcgcccag
     2101 atgtggaacc tgctcgcggg ctacgcgggc ctcgtctcga tcggccagca ggcctacgtg
     2161 ggcctcggcg gctacgccct catcgtgctg gccgacgacc tcggggtcga tccgttcgtc
     2221 gcggtgccgc tcgccggcct ggtgaccgct gccctcgcgc cgaccgcggc gctcgtcttc
     2281 cggttccggg gcggatactt cgcggtcggc acgtgggcca tcgccgaggt ctaccggtt
//
LOCUS       DATMCV010000611         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_231075, whole genome shotgun sequence.
ACCESSION   DATMCV010000611 DATMCV010000000
VERSION     DATMCV010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231075"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..930
                     /locus_tag="VNU03_14095"
     CDS             160..930
                     /locus_tag="VNU03_14095"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HWP75649.1"
                     /translation="MIKRVLMSVLVLAVAAGDVYAIDHTNLDEGRPLRLEDAYAIAEG
                     EIALEVGGGFTLPRRGSNQGLFPAEILYGAYPNLQLSVGTTLATDPRDIDDRPKSGDL
                     QVSALYNFNQETISLPAFALKVGVDTPTGVGARGYGVELKGIVTKSIERLSLHLNAGY
                     EFLIATSRGDRDGRYKGALGASYPIGAPQFTRATLVGDVFAEQSPRRGESTVVGVEVG
                     VRYQLTPRMVWDVGIGTEFAGPAHRADLFMTTGFSFGF"
     gene            981..>2205
                     /locus_tag="VNU03_14100"
     CDS             981..>2205
                     /locus_tag="VNU03_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWP75650.1"
                     /translation="MDVEPGQAGGGSAEHQGTAYWFCNPACREKFVADPGRYREARPS
                     AGGTADTRIYTCPMHPEVRQVGPGSCPKCGMALEPLDVIAADEGPSGELVDMTRRFWV
                     SLALTVPVLALAMSEMAAPALAERVSSTVRLWAQLVFSAPVVLWGGWPFFLRGWKSIV
                     TRNLNMFTLIALGTGTAFGYSVFAVLMPEALPPGMRHGGVPPVYFEAAAVITTLVLLG
                     QVLELRARSATSGAIRALLGLAPKTARRLRDDGRDEDVPLGHVQVGDRLRVRPGERVP
                     VDGIVLEGVSAVDESMVTGESIPVEKTPNSRVTGGTVNGTGSFVMRAERVGADTLLAQ
                     IVRMVSEAQRSRAPIQRLADVVAAWFVPVVVLIAAATAIGWGVFGPEPRLAYGLVNAV
                     AVLIIACPCALGLATP"
BASE COUNT          329 a          725 c          791 g          360 t
ORIGIN      
        1 gggacgcaga cgggcggaca cgcggccatg gcggagatgg gcccgcgacc gccttcggtg
       61 gaggaagtcc tcgctgggac cgccccggcg agcgatcagg cgcagccatc cgaccgctcg
      121 acccccgacc ctgagcacaa gcactgacgg gaacccacca tgatcaagcg ggtcctcatg
      181 tcagtgctcg tattggccgt ggcggccggc gatgtctatg cgatcgacca cacaaacctc
      241 gatgagggcc ggcccctgcg gctcgaagac gcctatgcca tcgcggaggg cgagatcgcc
      301 ctcgaggtcg gcggcggctt cacgctgccg cgacgcggct cgaatcaagg tcttttcccg
      361 gccgagatcc tctacggcgc gtatccgaac ctgcaattga gtgtcggcac caccctggcc
      421 actgacccgc gggacatcga cgaccgtccc aaatccgggg atctccaggt gagtgcgctc
      481 tacaacttca accaggaaac gatcagcttg ccggccttcg ccctgaaggt gggtgttgac
      541 acgccgacgg gtgttggtgc ccgcggatac ggtgtcgagc tgaaaggcat cgtcacgaag
      601 tccatcgagc gcctcagtct ccatctcaat gccggatacg agttcctcat cgcgacaagc
      661 cggggtgatc gagacgggcg ctacaagggc gcactgggcg cgagttatcc gatcggcgcc
      721 ccgcaattca cccgggcgac gctcgtgggc gacgtcttcg ccgagcagtc cccgcggcga
      781 ggcgagtcga ccgtcgtcgg agtggaggtc ggcgtacgat accagctaac tccccggatg
      841 gtctgggacg tggggatcgg gaccgagttt gccggaccgg cccatcgagc cgatctgttc
      901 atgacgacgg gcttctcgtt cggcttctga ccaggcgccg agtcgcggag ggatcgagcg
      961 tgaaagaccc agtgtgtggg atggacgtgg agccgggcca ggcgggaggc ggcagcgccg
     1021 agcaccaggg cacggcgtac tggttctgca atccagcctg ccgggagaag ttcgtcgccg
     1081 atcccggacg ctatcgtgag gcgcggccca gcgcgggtgg gaccgcggac acccgcatct
     1141 acacgtgccc gatgcacccg gaggtacgcc aggtcggtcc cggcagctgc ccgaagtgcg
     1201 gcatggccct cgagcccctt gacgtgatcg cggccgacga ggggccgagc ggcgagctgg
     1261 tcgatatgac ccggcggttc tgggtgagcc tggcactcac cgttccggtt ctggcgctgg
     1321 caatgtccga gatggcggcg cccgccctgg ccgagcgagt gagctcaacg gtgcgcctct
     1381 gggcccagct cgtgttctcg gcgccggtag tcctctgggg cggctggcca ttctttctcc
     1441 gcggctggaa gtcgattgtc acccgcaacc tgaacatgtt caccctgatc gcgctgggga
     1501 cgggcacggc gttcggctac agcgtgttcg ccgtgctcat gccggaagcg cttccccccg
     1561 ggatgcggca cggcggggtg ccgcccgtgt acttcgaggc ggccgcggtg atcaccacgc
     1621 tggtgctgct cggtcaggtg ctggagctgc gggcccgcag cgccacgtcc ggggcgatcc
     1681 gggccttgct cggcctggcc ccgaagacgg cgcggcggct ccgcgacgac ggccgcgacg
     1741 aggacgttcc gcttggtcac gtccaggtgg gcgatcggct gcgcgtgcgt ccgggcgagc
     1801 gtgtccccgt cgacgggatc gtgctcgagg gcgtgagcgc ggtcgacgaa tccatggtga
     1861 ccggcgagtc catcccggtg gagaagacgc cgaattctcg cgtcaccggc ggcaccgtga
     1921 acggcaccgg cagcttcgtg atgcgggcgg agcgggtcgg cgcggacacg ctgctcgcgc
     1981 agatcgtccg gatggtctcc gaagcccagc ggagccgtgc gcccatccag cggctggccg
     2041 atgtggtggc ggcgtggttc gtccccgtgg tcgtgttgat cgcggcggcc accgcgatcg
     2101 ggtggggcgt cttcggtccc gagccacggc tcgcctacgg cctggtcaat gcggttgcgg
     2161 tgctgatcat cgcgtgcccg tgtgcgctcg ggttggccac tccca
//
LOCUS       DATMCV010000612         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_829629, whole genome shotgun sequence.
ACCESSION   DATMCV010000612 DATMCV010000000
VERSION     DATMCV010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829629"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VNU03_14105"
     CDS             <1..1266
                     /locus_tag="VNU03_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP75651.1"
                     /translation="DRIVLLGPTIRGVDEHATPTTGDTRMWGVEILGNAVETVLYQHF
                     LVPASPSLTVALIGLMALAAAFVVAAWRPLLAGLAVAAGLALYGTAAAVLFERGLVLD
                     LVYPPAALLVSFAAALSSRLVVEQAEQRMVREAMGRYLSPVVSQWVLADPDRLRLGGE
                     TREMTVLFSDLRSFTTLAHALPPATLVALLNRYRAEMADVVFRHDGVLAQYAGDAIEA
                     FWNAPMDQADHARRACETALDMVAALAVLRPVFEARGWAHLDLGVGINTGQMVVGNMG
                     SKDRLIYTAVGDPVNVAARLEGLSKEYGVRVVVGEATVAAAPDAFAYRLLDLVEVKGR
                     GEPLRCYEVVARADTLRPAARQTLARYHEALELYRARRWEEALRLLDALAREAPADGP
                     VALYQRRARALLADPPPEDWDGVFVAESK"
     gene            complement(1270..2346)
                     /locus_tag="VNU03_14110"
     CDS             complement(1270..2346)
                     /locus_tag="VNU03_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75652.1"
                     /translation="MVCTRRLALLMIAALLSVPTGATGQERPGGVSTAATLTVLSVPV
                     ERVGVGSDRPAAVASGSDLAVGDRVVTGTDGRALITFLDGTTVTVEPASEVTVRQAQM
                     EGRQASRIGVLVTVGTVWARIAGWLGGRATVTIESNAYSATAHDGLIGAQQQPGGGPF
                     VCWTRAGTLEVAGAAGPAARLGPGQKATLVPGRPVLTESFAVNRSTIEITTSGPVLPL
                     VTMPGGPRLAGFVPPGIDVNQVFGSLTKAAPGGARTVEVPAGLSGPFVVTLTGVGDGP
                     FTVAVVGRYQGVVTYRHERAGIAHQGQSLRSEVVQLIDPPAAGAPDPRTTRAGSAGMS
                     ELRPEMGRPAPVPLSPLELAAPRP"
BASE COUNT          299 a          930 c          912 g          320 t
ORIGIN      
        1 gatcgcatcg tgctgctcgg ccccaccatc cgcggggtgg acgagcacgc cacgcccacc
       61 accggcgaca cccgcatgtg gggggtggag atcctgggca acgcagtgga gaccgtcctc
      121 taccagcact ttctggtccc ggcctccccc tcgctgaccg tggccctgat cgggctcatg
      181 gcgctggccg ccgccttcgt ggtggcggcc tggcgtccgc tgctcgccgg cctcgcggtg
      241 gcggccggcc tcgcgctcta cggcaccgcg gcggcggtgc tcttcgagcg cgggctcgtg
      301 ctcgacctgg tgtatccgcc cgccgcgctc ctggtgagct tcgcggccgc gctgtcctcg
      361 cgcctggtcg tcgagcaggc ggagcagcgg atggtgcgcg aggcgatggg gcgctacctc
      421 tcgccggtgg tgagccagtg ggtgctggcc gatcccgacc gcctgcggct cggcggcgag
      481 acccgcgaga tgacggtgct gttcagcgac ctgcgcagct tcaccacgct tgcccacgcg
      541 ttgcctccgg ccaccctcgt ggccctgctc aaccggtacc gtgcggagat ggccgacgtg
      601 gtcttccgcc acgacggggt gctcgcgcag tacgcgggcg acgcgatcga ggcgttctgg
      661 aacgctccga tggaccaggc cgaccacgcg cgccgcgcgt gcgagaccgc gctcgacatg
      721 gtcgcggccc tcgcggtgct gcggcctgtc ttcgaggcgc gcggctgggc ccacctcgat
      781 ctcggggtgg ggatcaacac cgggcagatg gtggtcggca acatgggctc gaaggaccgc
      841 ctgatctaca ccgcggtggg cgacccggtc aacgtggccg cgcggctcga ggggctctcg
      901 aaggaatacg gggtgcgggt ggtggtcggc gaggccaccg tggcggcggc tcccgacgcc
      961 ttcgcgtatc gcctcctcga cctggtggag gtgaagggcc gcggcgagcc gctccggtgc
     1021 tacgaggtgg tggcccgcgc cgacaccctc aggcccgccg cccgacagac gctcgcgcgc
     1081 taccacgagg ccctcgagct ctatcgcgcg cgtcggtggg aggaggcgct gcggctgctc
     1141 gacgcgctcg cccgcgaagc gcccgccgac gggccggtcg cgctctacca gcggcgcgcg
     1201 cgggcgctcc tggccgaccc gccccccgag gactgggacg gcgtcttcgt ggcggaatcg
     1261 aagtagcggc tacgggcgcg gcgcggccag ctcgagcggc gagagcggca ccggagcggg
     1321 ccggcccatc tcgggccgca gctcgctcat gcccgcgctg cccgcccgcg tggtccgagg
     1381 gtcgggcgcg cccgccgcgg gcggatcgat gagctggacg acctcggacc gcaggctctg
     1441 accctggtgc gcgatgcccg cgcgctcgtg ccggtaggtc accacaccct ggtagcgccc
     1501 caccaccgcc accgtgaacg ggccgtcgcc caccccggtc agcgtgacga cgaacggtcc
     1561 ggagaggccg gcgggcacct ccaccgtgcg cgcaccgccc ggcgccgcct tggtgagcga
     1621 gccgaacacc tggttgacgt cgatgccggg cgggacgaag ccggccagcc gcgggccacc
     1681 cggcatcgtc acgagcggca gcaccggccc cgacgtggtg atctcgatcg tgctgcgatt
     1741 caccgcgaag ctctcggtga ggaccggacg gccggggacg agcgtggcct tctggcccgg
     1801 accgaggcgc gccgccggac ccgccgcccc ggcgacctcg agggtgcccg cccgcgtcca
     1861 gcagacaaac ggcccgccac ccggctgctg ctgggcgccg atcagcccat cgtgcgcggt
     1921 ggcgctatag gcgttggact cgatcgtgac ggtggcgcgc ccgccgagcc agccggctat
     1981 ccgcgcccac accgtgccga ccgtgacgag cacgccgatc cgcgaggcct ggcgcccctc
     2041 catctgcgcc tggcggacgg tcacctcgga ggcgggctcc accgtgaccg tggtcccgtc
     2101 gaggaaggtg atgagggcgc ggccgtcggt gccggtgacc acgcggtcgc cgaccgccag
     2161 atcgctgccg ctcgccaccg ccgccggccg gtcggacccg acgccgaccc gctccaccgg
     2221 gacgctcagc acggtgaggg tggccgcggt ggagacgccg cccgggcgct cctgccccgt
     2281 cgccccggtc gggacggaca ggagcgcggc gatcatcagc agagccagtc gccgggtgca
     2341 caccatccgc ctcatcatac ccggcggggc tagagggacc ggggcccggc gctcccggat
     2401 cagcccggtc gggcgcgtcg gcctccgcga gagggtgcgg cattccgccg cccccggtac
     2461 g
//
LOCUS       DATMCV010000613         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_231230, whole genome shotgun sequence.
ACCESSION   DATMCV010000613 DATMCV010000000
VERSION     DATMCV010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231230"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VNU03_14115"
     CDS             <1..185
                     /locus_tag="VNU03_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP75653.1"
                     /translation="LTETVFAWPGIGRMLVAAVGQRDLPVVMGAVLVFAVIYVMANMV
                     VDLAYLVIDPRIRRGR"
     gene            175..1056
                     /locus_tag="VNU03_14120"
     CDS             175..1056
                     /locus_tag="VNU03_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75654.1"
                     /translation="MAASASALVRLRPERLAGVGGRRRGRLIVGAALVGLFAASALLA
                     PWLARYDPAAVPAALSDTMLRPPETAHLLGTDALGRDLLSRVLYGGRVSLTLGLGVEI
                     VTATLGVAIGLLAGYYGGWRDSLLMRVTDVVMAFPSLILAIGLIAVFEEPGLDKVALV
                     LVVLGWTTIARVVRGEVLSLKERDYVQAARALGVGHTRMLLRHLLPNAVGPVIVAATI
                     GVGSNMIAEAGLSFLGLGAQAPTISWGSMLAEGQTFLVSAPWVAFFPGAALLTAVLGI
                     NLLGDALRDLLDPRFKP"
     gene            complement(1338..>2075)
                     /locus_tag="VNU03_14125"
     CDS             complement(1338..>2075)
                     /locus_tag="VNU03_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP75655.1"
                     /translation="DHYVINGHKWWTSGIGDPRCKVLIFMGKTDAKNPDKYKQQSMIL
                     VPRDTPGIKVVRMLTVFGYDDAPHGHGEVLFENVRVPASNMLLGEGRGFEIAQGRLGP
                     GRIHHCMRQIGVAERAIELMCKRATKRIAFGKPLADNDITLERIAESRIEIDQARLLV
                     LHAAYMMDTVGNKAARQAIAEIKVAVPNITGRVVERAMQLHGGGGVSQEFPLASMWAH
                     SRTLRFADGPDEVHRRQIGRLELRKHS"
BASE COUNT          270 a          779 c          681 g          345 t
ORIGIN      
        1 cgctcaccga gaccgtgttc gcctggcccg gaatcggacg gatgctggtg gccgcggtcg
       61 gccagcggga cctgccggtg gtgatgggtg cggtgctcgt cttcgcggtc atctacgtga
      121 tggccaacat ggtcgtggac ctggcctacc tggtcatcga cccacggatc cgccgtggcc
      181 gctagcgcgt ccgcgctcgt gcgcctgcgg ccggagcggc tcgcgggggt gggcggccgg
      241 cggcgcggcc gcctcatcgt cggcgccgcg ctcgtcggcc tcttcgcggc gtccgctctc
      301 ctggccccct ggctcgcgcg gtacgacccg gccgcggtcc ccgccgcgct cagcgacacc
      361 atgctgcgcc cgcccgagac cgcgcatctg cttggcaccg acgcgctcgg ccgcgatctc
      421 ctctcgcgcg tgctctacgg aggccgcgtc tcgctcacgc tgggcctggg cgtcgagatc
      481 gtgaccgcga cgctcggggt cgcgatcggc ctcctggccg gctactacgg cggctggcgc
      541 gactcgctcc tgatgcgcgt gaccgacgtg gtgatggcct tcccgtcgct catcctggcc
      601 atcgggctga tcgccgtctt cgaggagccc ggcctcgaca aggtcgcgct cgtcctcgtg
      661 gtgctcggct ggacgaccat cgcgcgcgtg gtgcggggag aggtgctctc gctcaaggag
      721 cgcgactacg tccaggccgc ccgcgccctc ggagtcggcc acacccggat gctgctccgc
      781 catctgctcc cgaacgcagt cggcccggtc atcgtcgccg ccaccatcgg cgtcggaagc
      841 aacatgatcg ccgaggccgg cctctcgttc ctgggcctgg gcgcccaggc cccgaccatc
      901 tcctggggca gcatgctcgc cgagggtcag accttcctcg tctccgcccc ctgggtcgcc
      961 ttcttccccg gcgccgccct cctcaccgcc gtcctaggca tcaacctcct gggcgacgcc
     1021 cttcgcgacc tcctcgaccc ccgcttcaaa ccctaaccgc agcctcgaac aagaaggtgt
     1081 gaataagaaa atcgagcggc aaagccgcga ggccttaaac ccgcgagatt gagcaagcgg
     1141 aagggaggga gcgggcgggg ggaggggcgg cgggggtggg ccgggtgacc cgttccctcc
     1201 ggcgaccgcc tacaccgccc acttcccaga ttgggaactc ctcacgcaaa gaaaagagga
     1261 agcgggtcgt ggtcagaggc ccacccccgc cgcccctccc cccgcccgct ccctcccgct
     1321 tacgcgacgc cgaggagcta gctgtgctta cgaagctcga gccgcccgat ctgccgccga
     1381 tgcacctcgt ccggtccgtc cgcgaacctc agcgtccgcg agtgcgccca catcgacgcg
     1441 agcgggaact cctggctcac cccgccgccg ccgtgcagct gcatcgcccg ctccaccacg
     1501 cggccggtga tgttcggcac cgccaccttg atctccgcga tggcctggcg cgccgccttg
     1561 ttgccgacgg tgtccatcat gtaggctgcg tgcaggacca gcagccgcgc ctggtcgatc
     1621 tcgatgcgcg actccgcgat gcgctcgagg gtgatgtcgt tgtcggccag cggcttgccg
     1681 aaggcgatgc gcttcgtggc ccgcttgcac atcagctcga tcgcccgttc cgccaccccg
     1741 atctggcgca tgcagtggtg gatgcggccc ggacccagtc gcccctgggc gatctcgaag
     1801 ccgcggccct cgcccagcaa catgttggag gcgggcaccc gcacgttctc gaacagcacc
     1861 tcgccgtggc cgtggggcgc gtcgtcgtag ccgaagacgg tcagcatccg caccaccttg
     1921 atgccgggcg tgtcgcgcgg gaccaggatc atcgactgct gcttgtactt gtcggggttc
     1981 ttggcgtcgg tcttgcccat gaagatgagg accttgcagc gcgggtcgcc gatccccgac
     2041 gtccaccact tgtgcccgtt gatcacgtag tggtc
//
LOCUS       DATMCV010000614         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_632495, whole genome shotgun sequence.
ACCESSION   DATMCV010000614 DATMCV010000000
VERSION     DATMCV010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632495"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..261
                     /locus_tag="VNU03_14130"
     CDS             136..261
                     /locus_tag="VNU03_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75656.1"
                     /translation="MEGHLDDAQLARLQEAAEGCRISRAMKVPIQVPVRRATGNQ"
     gene            complement(268..1389)
                     /locus_tag="VNU03_14135"
     CDS             complement(268..1389)
                     /locus_tag="VNU03_14135"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP75657.1"
                     /translation="MASSGVVIVGGGIVGVVTAYYLARAGVASVVVERDAIGSHASGF
                     AYGGLSPVTGAGIPGPLAEVAQAGMRLHRELAESVVAETGIGIDFRVRRSLALAFTDA
                     EAQRLQGALPWQQQQPGYVSRWLDGAEARRVEPRISEEAIGAVSIEGGADVEPYRLVL
                     ALTRAAEALGASVRHGRITGLRREGGAVTHVVLERGEIACDTVVLALGPWSAEASSWL
                     DLPIEVRPLKGQILRLQAPGPPVPCSVGWGHNYATTKPDGLLWAGTTEEEAGFDEETT
                     PEARDEIGAALVKMLPAMADAQVVHQTACLRPVASDGLLLLGRVPGFDSVYMATGAGR
                     KGILLGPAMGRAVADLILTGKSALGLDAFSPARFARAPR"
     gene            complement(1393..2598)
                     /locus_tag="VNU03_14140"
     CDS             complement(1393..2598)
                     /locus_tag="VNU03_14140"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWP75658.1"
                     /translation="MDQALDDVTVLDLGQVIAMPFCTMLLADMGARVIKVESPERGRE
                     RVSLGVKRQRDGVEERVPAAQYRDRNKLGVTLDLKAPAGVELFRELVKHADVVSENFS
                     VGTMERLGLGYDDLRRVNPGLVYASITAFGQTGPYAPQRGYDMLAQAISGYMSITGDP
                     DGPPTRSGQSISDYYAGMLCAFSIVSALHYRQRTGRGQRIDLALLDSLVVALDNLGER
                     YTVGGEILTRAGNVSFAGSATGIYPAADGHVAVAASASNAIWARFCAVIGRDELTRDP
                     EFATAAARRDRRDRIAAIIQAWTGPRAKAEVVRVLSTAGVPAAPVNNVAEMVADPQVQ
                     AREMFVQRDHPVFGPLTTTGTPLKLSETPGTVRWLAPMPGEHNDEVFAGLLGHSRDDL
                     ERWRAEGVI"
     gene            complement(2610..>2897)
                     /locus_tag="VNU03_14145"
     CDS             complement(2610..>2897)
                     /locus_tag="VNU03_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP75659.1"
                     /translation="QHRITVNAIAPAQIDTRLTRTLPEEARRRRVERIPMGRFGEPEE
                     VARVALFLASDDASYVTGHTLAVDGGYLAGGLWSGAPGVVPPAAPPGVTPS"
BASE COUNT          391 a         1040 c         1092 g          374 t
ORIGIN      
        1 gccatcggcg cctgacgcat ctccaacgtt cggcggttcg ccgagcgcca gcagtggccg
       61 ctgcgcggcc ttcgcgcccg tctcaccgcg acggaagcac aggggcagat caccggcgtc
      121 gagggggagc tgctcgtgga gggccatctc gacgacgccc agctcgcccg cctccaggag
      181 gcggcggagg gctgccggat cagccgcgcg atgaaggtcc cgatccaggt gccggtgcgc
      241 cgcgcgaccg gcaaccagta gccgggatca gcgcggcgcg cgggcgaagc gcgccggcga
      301 gaaggcgtcg agaccgaggg cgctcttgcc ggtcaggatc aggtcggcca ccgcacggcc
      361 catcgcgggg ccgagcagga tgcccttgcg gcccgcgccg gtggccatgt agacggagtc
      421 gaagcccggc acccgcccga gcagcagcag gccgtcggag gccaccggcc gcagacaggc
      481 ggtctggtgc accacctggg cgtcggccat cgcgggcagc atcttcacca gcgcggcgcc
      541 gatctcgtcg cgggcctcgg gcgtcgtctc ctcgtcgaag ccggcctcct cctcggtggt
      601 gcccgcccac aggaggccgt ccggcttggt ggtggcgtag ttgtggcccc agccgacgga
      661 gcaggggacc ggcggcccgg gcgcctgcag gcgcaggatc tggcccttga ggggacggac
      721 ctcgatcggc aggtcgagcc aggacgaagc ctccgcgctc cacgggccga gggccagcac
      781 caccgtgtcg caggcgatct caccccgctc gaggaccacg tgcgtgaccg ctccgccctc
      841 gcgtcggagg ccggtgatgc gcccgtgacg cacgctcgcc ccgagcgcct cggcggcgcg
      901 ggtgagggcg agcacgaggc gatagggctc gacgtccgcg ccgccctcga tcgagaccgc
      961 gccgatggcc tcctcggaga ttcgcggctc cacgcgccgg gcctcggcgc cgtcgagcca
     1021 gcgcgagacg tagcccggct gctgttgctg ccagggcagg gccccctgga gccgttgcgc
     1081 ctccgcgtcg gtgaatgcca gcgccagcga tcggcgcacc cggaagtcga tgccgatccc
     1141 ggtctccgcc accacgctct cggccagctc gcgatggagc cgcatccccg cctgcgccac
     1201 ctcggcgaga gggccgggga tgccggcgcc ggtcaccggg ctcagcccgc cgtaggcgaa
     1261 gccggacgcg tggctgccga tggcgtcgcg ctccaccacg acgctggcga cgccggcgcg
     1321 ggccaagtag taggcggtga ccactcccac gatgccgccg ccgacgatca ccaccccgct
     1381 cgaggccatg gctcagatca ccccttccgc gcgccagcgc tcgaggtcgt cgcgcgagtg
     1441 gcccaggagt ccggcgaaga cctcgtcgtt gtgctcgccg ggcatgggcg ccagccagcg
     1501 aacggttcca ggcgtctccg agagcttgag cggcgtcccg gtggtggtca gcgggccgaa
     1561 gacgggatgg tcgcgctgca cgaacatctc gcgggcctgc acctgcgggt cggcgaccat
     1621 ctcggccacg ttgttgaccg gcgcggcggg gacgccggcc gtcgagagga cgcgtaccac
     1681 ctccgccttg gcgcgcgggc cggtccacgc ctggatgatg gcggcgatcc ggtcgcggcg
     1741 gtcgcgccgg gccgccgcgg tggcgaactc gggatcgcgc gtcagctcgt cgcgcccgat
     1801 gaccgcgcag aagcgcgccc agatcgcgtt gctggccgag gcggcgaccg cgacgtggcc
     1861 gtcggcggcc ggatagatcc cggtggcgga gcccgcgaaa gagacgttgc ccgcgcgggt
     1921 gaggatctcg ccgcccaccg tgtagcgctc gccgaggttg tcgagcgcca ccaccaggct
     1981 gtccagcagg gccaggtcga tgcgctggcc ccggccggtg cgctgccggt agtgcagcgc
     2041 ggagacgatg ctgaacgcgc agagcatgcc cgcgtagtag tccgagatgg actggccgga
     2101 gcgggtgggc ggcccgtcgg gatcgccggt gatgctcatg tacccgctga tcgcctgcgc
     2161 gagcatgtcg tagccgcgct ggggtgcgta ggggccggtc tggccgaacg cggtgatcga
     2221 cgcgtagacg aggccggggt tcacgcgacg caggtcgtcg tagccgaggc cgaggcgctc
     2281 catggtgccc acgctgaagt tctcgctgac cacgtcggcg tgcttgacca gctctcggaa
     2341 gagctcgacg ccggccgggg ccttcagatc cagggtgacg ccgagcttgt tgcgatcgcg
     2401 atactgcgcg gccggcaccc gctcctccac gccgtcgcgc tgccgcttca cgccgagcga
     2461 gacgcgctcg cggccgcgct ccggcgactc gaccttgatc acccgcgcgc ccatgtcggc
     2521 gagcagcatc gtgcagaagg gcatcgcgat gacctggccc agatcgagca ccgtgacgtc
     2581 gtcgagggcc tgatccaccg ggagcggacc tagctgggcg tgacgccggg cggggcggcg
     2641 ggcgggacga cgccgggggc gccggaccag aggcctccgg ccaggtagcc gccgtccacc
     2701 gcgagcgtgt ggccggtgac gtagctggcg tcgtcggagg cgaggaagag cgcgacccgg
     2761 gcgacttctt cgggctcacc gaagcgcccc atcgggatgc gctccacccg ccggcgccgc
     2821 gcctcttccg gcaaggtccg ggtgagccgg gtgtcgatct gggccggcgc gatcgcgttc
     2881 accgtgatgc ggtgctg
//
LOCUS       DATMCV010000615         3199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_299981, whole genome shotgun sequence.
ACCESSION   DATMCV010000615 DATMCV010000000
VERSION     DATMCV010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299981"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..776
                     /locus_tag="VNU03_14150"
     CDS             102..776
                     /locus_tag="VNU03_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75660.1"
                     /translation="MTLEVRPGERRALIGPNGAGKTTLFNLISGAMPPSSGRISLFGE
                     DVTGAPAHRRAALGLARTFQITNLFPDLSVLENCLLAVQAHTPTRFAMHRPMGRYTAL
                     LDRARATLASLELGALADAVVKNLSHGEQRQLEIALALAGRPKVLLLDEPTAGLSPAE
                     SRLMAGLLRRLDPAITVLMIEHDMDIALELSEMVAVLHYGRVIADGPRERVKADPQVK
                     EIYLGA"
     gene            766..1461
                     /locus_tag="VNU03_14155"
     CDS             766..1461
                     /locus_tag="VNU03_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75661.1"
                     /translation="MALEVEAIHTYYGESHVLHGISLRVDPGEVLAILGRNGMGKTTL
                     VRSIVGFTPPRDGHIRYKGAEITRLPSFRMVELGMALVPQGRRVFPSLSVRENLEVAR
                     RGTGRWSLEQVYAVFPRLRERAANRANKLSGGEQQMLAIGRALMSNPELLLLDEPTEG
                     LAPLLVREVGRVLAELKRSGLSILLVEQNLALALSVADRVHVLSRGQIVHSGTPDDLM
                     TNEDVKTRYLGVA"
     gene            1461..2522
                     /locus_tag="VNU03_14160"
     CDS             1461..2522
                     /locus_tag="VNU03_14160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75662.1"
                     /translation="MRFDTYFFLQATPGRRHAEIIQTELDQMVLSEELGFDEVWLTEH
                     HFIDYGLSVDPATLASAVASRTSRLRIGLAAAILPFHDPIRIAEQLALVDIISGGRLD
                     VGVGRGNRPSEFIGYRVPQVESRERFDEAVQIIQRAWTAERFSYDGRYFQIPEVRVIP
                     KPLQRPHPPLYQVCVSPDSIEGTALRGWPMLNSLLFGGVEQLVATRDRYVATLQKAGR
                     SADDIAALLALWGVSRQIYVAPTDAHALEEARDAEMWYQESFRKFLIPERIEASHPTL
                     QPGFRAMAERLSKITWEGLVAETLAFGSPDTVARHIEQMRQMGVGQLMCWMNFGGLPE
                     DKVRRSMDLFAREVLPRFK"
     gene            complement(2616..>3199)
                     /locus_tag="VNU03_14165"
     CDS             complement(2616..>3199)
                     /locus_tag="VNU03_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75663.1"
                     /translation="GTRIERLTEAVTIVKGLLGDAPVTVAGRHYTITALQGRPSPVQR
                     PHPPILIGGGGPRTLSLAAREASIVGLVPRARRDGGGLDRGDFGEAALREKIGWVRDA
                     AGDRFEALELHALIQAVIVSDQRSVAAEQVAARFGSGREVLLESPYVVIGSVDGICET
                     LRQRREAYGISYLSVFDRDLEVFAPVVERLAGI"
BASE COUNT          472 a         1179 c         1077 g          471 t
ORIGIN      
        1 ctggggagag ggcagggtga ggggcccaga ggtgaggggc ccccgccctc cgcctcgaca
       61 acgtctgccg ctcctttggc ggcctgcagg cggtggacgg cgtgacgctc gaggtgcgcc
      121 cgggcgagcg ccgcgccctc atcggcccca acggcgcggg caagaccacg ctcttcaacc
      181 tgatctcggg cgccatgccg cccagctcgg gccgcatctc cctcttcggc gaagacgtca
      241 ccggcgctcc cgcccaccgc cgcgcggcgc tcgggctcgc ccgcacgttc cagatcacga
      301 atctcttccc cgacctgagc gtgctggaga actgcctgct cgcggtgcag gcccacaccc
      361 cgacgcgctt cgcgatgcac cgcccgatgg gacgctacac cgcgctgctc gaccgcgcgc
      421 gggccaccct cgcctcgctc gagctcggcg ccctggccga cgcggtggtg aagaacctct
      481 cgcacggcga gcagcgccag ctcgagatcg cgctggccct ggccgggcgg ccgaaggtgc
      541 tgctgctgga cgagcccacc gccgggctct cgcccgcgga gtcgcggctc atggccgggc
      601 tgctgcggcg gctcgacccc gcgatcacgg tgctgatgat cgagcacgac atggatatcg
      661 cgctcgagct gtccgagatg gtcgcggtgc tccattacgg gcgcgtcatc gccgacggcc
      721 cgcgcgagcg ggtgaaggcg gatccccagg tgaaggagat ctaccttggc gcttgaggta
      781 gaggccatcc acacgtacta cggcgagagc cacgtgctcc acggcatctc gctgcgggtg
      841 gacccgggcg aggtgctggc catcctcggg cgcaacggca tgggcaagac cacgctggtg
      901 cgctccatcg tgggcttcac cccgccgcgc gacggccaca tccgttacaa gggcgccgag
      961 atcacccggc tgccgtcgtt ccgcatggtg gagctgggca tggccctcgt gccgcagggc
     1021 cggcgggtgt tcccgtcgct gagcgtgcgc gagaacctcg aggtcgcccg ccgcggcacc
     1081 gggcgatgga gcctggagca ggtgtatgct gtgtttccgc gcctgcggga gcgggcggcc
     1141 aatcgggcca acaagctctc ggggggcgag cagcagatgc tggcgatcgg acgggcgctg
     1201 atgtcgaacc ccgagctgct gctgctggac gagccgaccg aggggctggc gcccctgctg
     1261 gtgcgcgagg tgggccgggt cctcgcggag ctgaagcgga gcgggctgtc gatcctgctg
     1321 gtggagcaga acctggccct cgcgctctcg gtggccgatc gggtgcacgt actgagtcgc
     1381 gggcagatcg tccacagcgg cacgccggac gatctcatga ccaacgaaga cgtgaagaca
     1441 cggtacctgg gagtcgcgtg atgcgcttcg acacctactt cttcctacag gccacccccg
     1501 gccgtcgcca cgccgagatc atccagaccg agctggacca gatggtgctc tcggaggagc
     1561 tgggcttcga cgaggtgtgg ctcaccgagc atcacttcat cgattacggc ctctcggtgg
     1621 acccggccac gctggcctcc gcggtggcct cgcggacgtc gcgcctgcgc atcgggctgg
     1681 ccgccgcgat cctgcccttc cacgacccga tccgcatcgc cgagcagctg gccctggtcg
     1741 acatcatctc gggcggccgc ctcgacgtgg gcgtgggacg cggtaaccgg ccgtcggagt
     1801 tcatcggtta ccgggtgccg caggtggaga gccgcgagcg cttcgacgag gcggtgcaga
     1861 tcatccagcg cgcgtggacc gcggagcgct tctcgtacga cggtcgttac ttccagatcc
     1921 ccgaggtgcg ggtgatcccg aagccgctgc agcgcccgca cccgccgctc taccaggtgt
     1981 gcgtgtcgcc cgactccatc gagggcaccg cgctgcgcgg ctggcccatg ctcaactcgc
     2041 tgctcttcgg tggcgtggag cagctcgtgg ccacccgcga ccgctacgtg gccacgctcc
     2101 agaaggccgg gcgcagcgcg gacgacatcg ccgccctgct cgcgctctgg ggcgtctcgc
     2161 ggcagatcta cgtggcgccc accgacgcgc acgcgctgga ggaggcccgc gacgcggaga
     2221 tgtggtacca ggagtccttc cgcaagttcc tcatccccga gcgcatcgag gcgagccacc
     2281 cgacgctgca gcccggcttc cgcgccatgg ccgagcgcct ctcgaagatc acgtgggagg
     2341 ggctcgtggc cgagacgctg gccttcggct cgcccgacac ggtggcgcgc cacatcgagc
     2401 agatgcgcca gatgggcgtg ggccagctca tgtgctggat gaacttcggc ggcctccccg
     2461 aggacaaggt ccgccgctcc atggacctct tcgcccgcga agtcctcccg cgcttcaagt
     2521 aaccagcgga ttcaagtaac cctctccctc atgggattca ataaccctct cccctaaggg
     2581 gagagggtag ggtgaggggt ggcggctcga acccctcaaa tccccgccag ccgctccacc
     2641 accggcgcaa aaacctcgag atcccgatcg aacacgctga gatacgagat cccatacgcc
     2701 tcccgcctct gccgcagcgt ctcgcagatc ccatccacgc taccgatcac cacgtaagga
     2761 ctctccagca agacctcgcg cccggacccg aatctcgccg ccacctgttc cgcggcgacg
     2821 gaccgctgat cgctcacgat caccgcctgg atcagcgcgt gtagctcgag cgcctcgaat
     2881 cgatcccccg ccgcgtcgcg gacccacccg atcttctccc ggagcgccgc ctcgccgaag
     2941 tccccgcgat ccaggccgcc gccgtcccgc cgcgcgcgcg gcaccagccc gacgatcgag
     3001 gcctcgcgcg ccgccagcga gagcgtgcgc ggcccgcccc cgccgatcag gatgggcgga
     3061 tgcggccgct ggaccgggct cgggcgcccc tgcagcgccg tgatcgtgta gtgccggccc
     3121 gcgaccgtca ccggcgcgtc acccagcagc cccttgacga tggtgacggc ctcggtaagc
     3181 cgctcgatcc gcgttcccg
//
LOCUS       DATMCV010000616        11606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_766518, whole genome shotgun sequence.
ACCESSION   DATMCV010000616 DATMCV010000000
VERSION     DATMCV010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11606
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766518"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VNU03_14170"
     CDS             <1..262
                     /locus_tag="VNU03_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP75664.1"
                     /translation="GIRVNCIAPGPIETEGYLGVLTKQDPAVAKKAYDAVASRVGMGR
                     WGKVHEIAYPCIFLASDASAFMNGATIVVDGGPSAREGDEAS"
     gene            344..1531
                     /locus_tag="VNU03_14175"
     CDS             344..1531
                     /locus_tag="VNU03_14175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWP75665.1"
                     /translation="MREAVVVASSRTPLAKSFRGSFNMTRPDDLAAHCIKDVLGKVPR
                     LDSSEIEDVILGCAQPNGPQGSNVARVALIRAGLPASVAGTTVNRFCSSGLQAIAMAA
                     HQIILGGAEAAIGGGLESISMMKRDGDPNPWVKEKKPGIYMVMGDTAEVVAKRYNVTR
                     QMQDEYSLVSQQRTARAQQDGFFKEEISPMQVVRGIVDKKTGEITGKEDGYVDKDECN
                     RPDTTLEGLLALQPHFDKTSGKGSVTAGNSSQLSDGASATLLMSADRAKALGIKPKLI
                     FRGFAVAGCEPDEMGIGPIYAVPKLLKRNGLKMDDIDVWELNEAFASQVVYCRDKLGI
                     PMEKLNLNGGSISIGHPFGMTGSRMVGTIANEMLRRKAKYGVVTMCIGGGQGAAGLFE
                     SCL"
     gene            1688..2845
                     /locus_tag="VNU03_14180"
     CDS             1688..2845
                     /locus_tag="VNU03_14180"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HWP75666.1"
                     /translation="MAYDFDRVIERRPTNSNKWRKYPADVLPLWVADMDFPSPEPVVR
                     ALRERVEHGVYGYGFEGPEFSEVFVDRVQKRYGWTISPEALVMIPGVIPGFNVACHAL
                     TKPGDGLLMQLPVYPPILRSPTNHELTRDGAPLGRGRDGRYVADLDAFRAAIHPRTRA
                     FILCNPHNPVGRVFTREELAGMAQVCLEHDIPIIADEIHCDLLFASQRHIPVASLAPE
                     IERRTITLMAPSKTFNLAGLKVSVAVIPDASLRERFMAARGDYVQTQVNILGYAGALA
                     AYRDGQEWLDELMRYLEGNRDFLVDYVRRHLPGVSMVAPEGTYLAWLDCRNAGPAAAD
                     PFTFFLERAKVALNDGALFGAGGQGFVRLNFGTPRALLTEGLERMRRALGA"
     gene            3008..3307
                     /locus_tag="VNU03_14185"
     CDS             3008..3307
                     /locus_tag="VNU03_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75667.1"
                     /translation="MHARTGMTAVALATMMLYSGCATAPRGPSVQVLPGTGKPFEQFQ
                     ADVEVCRAWSAQQVKGAFMDAPSWEVQRRYDNAYVQCMYAKGHQVPSPPGPPRAR"
     gene            3321..5573
                     /locus_tag="VNU03_14190"
     CDS             3321..5573
                     /locus_tag="VNU03_14190"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HWP75668.1"
                     /translation="MITLRRSLATLLSLTLLVGSAPGAWAADAPAAPPPPPAGAPPAP
                     PAAAPPATSPADSAAAPDDGQPAPDQPEGAAPTPPRLSYTDGDVSFWRPGGDDWAPAQ
                     VNTPLSPGDQLYTGNRGNLEIQIEARNFVRAWGDSQLGVENHDPDFLQLKVAAGHVSL
                     DLRALDPGHTVELDTPGAAFTIDRPGYYRVDVAPDRTSFVTRRGGVATMTPAGGAAAS
                     IATSEQVVVTGTGPNPVQTFVAPELDVWDRWNYARTEALIDTVSARYVPAGLSGVDDL
                     DQHGAWRVVPEYGSVWVPQGVATDWAPYTTGRWVWDPRFGWSWVDTAPWGWAPYHYGR
                     WVHLDRVWAWAPGPVIVRPVYAPALVAFFGAPGVRVAIGAPFVSWVALGWGEPCVPWW
                     GRPGFVGRAWWGGWGGPRVVNNVVVNRTTVVNVRNITVYRNVGVQNAVVAVRPDRFGR
                     GPVGDARIRQVDTRRLEPVRGAVDLRPDRASFAAASGRGVRPPESVLARPTVATRAPV
                     TRALPHEAEGRGPAPRIVPAPHAAEPGAASARPPFGESRLERARPPQAPRFDTNRRPD
                     ATPPATPPAARRQDGGPDAARGRGPASPRTEAPRGAEVPRGPEPSRAPQAAVPAEAPR
                     VPQAQAPRSPQPQAPRQSEIQRGAQPPARAPEAAAPRRAPDAARAPRPEGPGARGAEP
                     RPAAPTPRAETPRQAPPRALPGEPANRLYPGRVERPSSRGPETRPQARPRPAPEHNTG
                     RTGGAAREAR"
     gene            complement(5575..6801)
                     /locus_tag="VNU03_14195"
     CDS             complement(5575..6801)
                     /locus_tag="VNU03_14195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP75669.1"
                     /translation="MANGLASHGRRSAVAIARANPVALYFREIGAVDRLTAAQERERG
                     RWIEICRARRRRALAGLPAGIDALLAATREVGRGDRSLDALLLLPGGASLTVRQRDRI
                     FEILARLRRMRPRLTHSAIARRSAGRLIERLPLRPDVVDDLVGELRQANGPIPARWRP
                     ALREVEESDRALREAKRALVEPNLRLVVSIARRYAGGSLTLLDLVQEGNIGLMKAVER
                     FDYHRGFKFSTYATWWIRQAVGRALADQARTIRMPVHIIESMHRLMRERNAFQAEHER
                     EPSVEELAQRTGVSEKTVRLVDLSAATPVSLDAPIGQDSTLGEFVRDRVTPSPEETIV
                     RADRSHQLGIAIESLPPREREVLRLRFGLDGEETLTLEEVGARFRVTRERARQLEAQA
                     LRRLGRSLAGEWAPGS"
     gene            complement(6822..8282)
                     /locus_tag="VNU03_14200"
     CDS             complement(6822..8282)
                     /locus_tag="VNU03_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HWP75670.1"
                     /translation="MNRLTMRYRAAWTALIVAAVVGLVALAPHLGFTGSAPAPIWSER
                     PVSVTAAATVPAPNWVELTRALKPAVVNISTKRVQDGTSMQSPFGNDDPFQQFFKQFG
                     QPGRKTVRSMGSGFVINPAGYIMTNNHVVDGATEIQVKIDDGRELPGKVVGRDPRTDL
                     ALLKVEATNLPVIPLGDSAALQVGEPVMAIGNPFGLERTVTTGIVSATGRVIGQGPYD
                     DFIQTDASINPGNSGGPLINARGQAVGINAAIFSQSGGSVGIGFAIPINQAKSVVTQL
                     AATGKVTRGWLGVMIQPLTPELAKGFNVPPGGGALVASVQPDSPAARAGVKAGDVITE
                     FDGHKLPQPGDLSKLVAATAVGRDVPLTVVREGKTMALQTRIARMDEPDQPALAQRDE
                     GKEPLGLTVETLTPDVGRQLGLADARGVVVRGVRDSSPAEKAGLRPGDVITELDHQPV
                     ASAADMKRLLAKHAKGAPVVVLLHRDGNSLYAALTS"
     gene            complement(8309..8884)
                     /locus_tag="VNU03_14205"
     CDS             complement(8309..8884)
                     /locus_tag="VNU03_14205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP75671.1"
                     /translation="MPQTWISRSPWIGRLAMGGVLAAALAALASGPAWWRAHGLVESG
                     ATLVSDGEYLPAVRQLVQAVAAAPQDARAHYYLGLAYAGLGQDAAALSHAEDAVRLAP
                     RDARYETGLATLFLDAGRTPEAIAHLRRAVDMKPTSPDIRLLLADALRRAGDTVGMER
                     EYRIAMRLAGGDTLGALAREQLRAAREQAAP"
     gene            complement(8941..9351)
                     /locus_tag="VNU03_14210"
     CDS             complement(8941..9351)
                     /locus_tag="VNU03_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75672.1"
                     /translation="MSTETEVPAQLTAFGHRSQPAQAVILEQAPGQRFVRTLTGLGMF
                     WGLGLASVFIPVAHFVLVPTFVVAGIVMAVKRAREDRRLLLVRGACPRCGAVQELRPG
                     GRFVDGRSFDCPKCHGNLTLVTASAEPAPAHSPR"
     gene            complement(9422..10831)
                     /locus_tag="VNU03_14215"
     CDS             complement(9422..10831)
                     /locus_tag="VNU03_14215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HWP75673.1"
                     /translation="MRGLRLPLSIRTKLTLWYSGVLLAILVTIAAISYSWLSFGLQQD
                     LDASLLTVAQVIKDTGSSEQASGDSDALLRQLLGPELYDKFVQLLDPEGRPHAPLAGR
                     RADVLPLSPQARARAARGLRTIETVVVGGLEPVRLLTLPVVRDGRVTEMIQVGMALRR
                     TQAVRDRYLDTLIVLIPLGVGLAALGGAIIARAALRPVDEMARTARRITAEDLSRRVE
                     RRESGDELDALAETLNGMLARLDDAFGQTRRFAADAAHELRTPLTVLRGGIEVALRME
                     RSPDEYRRVLASSLEEVERLIRLAEDLLLLSRSLAGPEGARAPVDLEPLVLEVFEVGA
                     RIGRAAGVSVRVDATVPARVTGDAAALRRALLNLVENAIKYTPSGGKVEIGLGRGDGV
                     AEVTIADSGIGIEADDLERIFEPFLRLDAARARDTGGAGLGLAIARSIVIAHGGAITV
                     ESRPGSGSRFTVRLPSTAA"
     gene            complement(10828..11505)
                     /locus_tag="VNU03_14220"
     CDS             complement(10828..11505)
                     /locus_tag="VNU03_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWP75674.1"
                     /translation="MRILVVEDERKVAAFIRQGLVEEGHAVAVASDGAAALDAIAEDP
                     AYDLVVLDVMLPKRDGFDVLRTLRERRVQTPVLLLTARDGVADKVAGLDLGADDYLTK
                     PFAFEEFVARVRALLRRSPGRAEPRLQVADLSLDPATRAVTRGGRRITLTAREYALLE
                     YFLRNADRVLTRPMITQHVWGMDFDPESNVVDVYVGYLRRKIDAEGEARLLQTVRGAG
                     YMLSAES"
BASE COUNT         1608 a         4274 c         4185 g         1539 t
ORIGIN      
        1 cggcatccgc gtcaactgca tcgccccggg accgatcgag accgagggct acctcggcgt
       61 gctgaccaag caggatccgg ccgtggccaa gaaagcctac gatgccgtgg ccagccgcgt
      121 gggcatgggg cgctggggca aggtccacga gatcgcctat ccctgcatct tcctggcctc
      181 tgacgcctcg gccttcatga acggcgccac catcgtggtc gacggcggcc cgagcgcccg
      241 cgagggcgat gaggcctcct agcaagtgac cggcaggcgc taaaggcgta tactcccgtc
      301 aaccaacggc atttcgagcc accggattca gaaaggagcc gccatgagag aggctgtcgt
      361 cgtcgccagc tcgcgcaccc cgctcgccaa gtccttccgc gggtcgttca acatgacccg
      421 gccggacgac ctggccgctc actgcatcaa ggacgtcctg ggcaaggtcc cgcgtctcga
      481 ttcctccgag atcgaggacg tcattctcgg ctgcgcccag cccaatggcc cgcagggcag
      541 caacgtggcg cgtgtggcgt tgattcgcgc cgggctcccc gcgtcggtcg ccgggaccac
      601 ggtcaaccgc ttctgctcgt ccgggctgca ggcgatcgcc atggccgcgc accagatcat
      661 cctgggcggg gcggaggccg ccatcggcgg cgggctcgag agcatcagca tgatgaagcg
      721 cgacggcgat ccgaacccgt gggtgaagga gaagaagccc ggcatctaca tggtgatggg
      781 cgacaccgcg gaggtggtgg ccaagcgcta caacgtcacc cgccagatgc aggacgagta
      841 ctccctggtg agccagcagc gcaccgcgcg ggcccagcag gatggcttct tcaaggaaga
      901 gatctcgccg atgcaggtcg tgcgcggcat cgtggacaag aagaccggcg agatcaccgg
      961 caaggaagac ggctacgtgg acaaggacga gtgcaaccgt cccgacacca cgctcgaggg
     1021 cctgctcgcg ctgcagcctc acttcgacaa gacgagcggc aagggcagcg tcaccgcggg
     1081 caactcctcg cagctctccg acggggcctc cgccaccctc ctaatgtcgg cggaccgggc
     1141 caaggccctc ggcatcaagc ccaagctcat cttccgcggg ttcgcggtcg ccggctgcga
     1201 gccggacgag atgggcatcg ggccgatcta cgcggtgccg aagctgctca agcgcaacgg
     1261 cctcaagatg gacgacatcg acgtgtggga gctgaacgag gccttcgcct ctcaggtcgt
     1321 ctactgccgc gacaagctgg gtatcccgat ggagaagctc aacctgaacg gcggctcgat
     1381 ctcgatcggc caccccttcg gcatgaccgg ctcccgcatg gtcggcacca tcgccaacga
     1441 gatgctgcgc cgcaaggcga agtacggcgt ggtcacgatg tgcatcggcg gcggccaggg
     1501 cgcggccggc ctcttcgagt cctgtctcta gtctctacgc gttgaattcg cccctcaccc
     1561 tgccctctcc ccttcggggc gagggtggcc gatatccctc tcgccttcgg ggcgagggtg
     1621 gcatcgaacc ctctcccccc tggggagagg gcagggtgag gggcgctctc gtctcccggt
     1681 gacccccatg gcctacgact tcgaccgcgt catcgagcgc cgccccacca acagcaacaa
     1741 gtggcgcaag tacccggccg acgtcctgcc actctgggtg gcggacatgg acttcccctc
     1801 gcccgagccg gtggtgcggg cgctgcggga gcgggtcgag cacggcgtct acggctacgg
     1861 cttcgagggg ccggagttca gcgaggtctt cgtggaccgc gtgcagaagc gctacggctg
     1921 gacgatctcg ccggaggcgc tggtcatgat ccccggcgtg atcccgggct tcaacgtggc
     1981 ctgccacgcc ctcaccaagc cgggcgacgg cctgctgatg cagctgccgg tctatccccc
     2041 gatcctccgg tccccgacca accacgagct cacccgcgac ggggccccgc tcggccgcgg
     2101 gcgcgacggc cgctacgtgg ccgatctcga cgccttccgc gcggcgatcc atccccgcac
     2161 gcgcgcgttc atcctctgca acccgcacaa cccggtcggc cgcgtgttca cgcgcgagga
     2221 gctcgccggg atggcccagg tctgcctcga gcacgacatc ccgatcatcg cggacgagat
     2281 ccactgcgac ctgctcttcg ccagccagcg ccacatcccg gtcgcctcgc tcgcgcccga
     2341 gatcgagcgg cgcaccatca ccctgatggc gcccagcaag accttcaacc tggccggcct
     2401 caaggtctcg gtggcggtga tcccggacgc ctcgctgcgc gagcggttca tggccgcgcg
     2461 cggcgactat gtgcagacgc aggtcaacat cctgggctac gcgggggcgc tcgccgccta
     2521 tcgcgacggg caggagtggc tcgacgagct gatgcgttat ctcgagggca accgggactt
     2581 cctggtcgac tacgtccggc ggcacctgcc cggcgtctcg atggtggcac ccgagggcac
     2641 ctacctcgcc tggctcgact gccgcaacgc gggcccggcc gcggccgatc cgttcacgtt
     2701 cttcctggag cgcgcgaagg tggcgctgaa cgacggagcc ctcttcggcg ccggcggaca
     2761 gggcttcgtg cgcctcaact tcggcacgcc ccgcgcgctg ctgaccgaag ggctcgagcg
     2821 gatgcgccgg gccctcggcg cctgaggccg cgcccgccgg cccgacgagt tggacgcggc
     2881 cgcgcccgcg gccgtttaca gggcgatccg gaagtttctt cccccgaatg gggctcggat
     2941 gggcctcgtc tgactgcgtg aaaccctgta tctgcccaat cttctgccca atgctctgga
     3001 ggtggcgatg cacgcacgaa cgggcatgac cgcggtggcg ctggcgacca tgatgctgta
     3061 ctcgggctgc gcgaccgccc cccgcgggcc gagcgtccag gtcctgcccg gtactggcaa
     3121 gcccttcgag cagttccagg ccgacgtgga ggtctgtcgc gcctggtcgg cccagcaggt
     3181 caagggcgcc ttcatggacg ccccgtcgtg ggaagtgcag cgccgctacg acaacgccta
     3241 cgtgcagtgc atgtacgcga agggccacca ggtgccgtct cctccgggac caccacgcgc
     3301 acgctgacgg aggcaatgag atgatcacgc tcaggcgatc gctcgcgacc ctgctgtcgc
     3361 tcacgctgct cgtgggaagc gcccccgggg cgtgggcggc cgacgcaccc gccgctccgc
     3421 cgcccccgcc cgcgggcgcg ccgcccgcgc cgccggccgc ggccccgccg gccacttcgc
     3481 cggccgattc ggccgccgct cctgatgacg ggcagcccgc gcccgatcag ccggagggcg
     3541 ccgccccgac gccgccgcgg ctcagctaca ccgacggcga cgtgtcgttc tggcgcccgg
     3601 gcggcgacga ctgggccccc gcgcaggtca acaccccgct cagccccggc gatcagctct
     3661 acaccggcaa ccggggcaac ctcgagatcc agatcgaggc gcgcaacttc gtgcgggcct
     3721 ggggcgacag ccagctcggc gtggagaatc acgatccgga cttcctccag ctgaaggtcg
     3781 cggccgggca cgtgtcgctg gacctgcgcg cgctcgaccc gggccacacc gtggagctgg
     3841 acactcccgg cgccgcgttc accatcgatc ggcccggcta ctaccgcgtg gacgtcgcgc
     3901 cggatcgtac ctcgttcgtc acccggcgcg gcggcgtcgc gacgatgacc ccggccgggg
     3961 gcgcggcggc ttccatcgca acgagcgagc aggtcgtcgt gaccggaacc ggtcccaacc
     4021 cggtgcagac cttcgtggcc cccgagctcg acgtgtggga tcgctggaac tacgcgcgca
     4081 ccgaggccct catcgacacg gtgagcgcgc gctacgtccc ggccgggctc tcgggcgtcg
     4141 acgatctgga ccagcacggc gcctggcgcg tggtgccgga gtacggctcg gtgtgggtgc
     4201 cgcagggcgt cgcgacggac tgggccccgt acaccaccgg gcgctgggtg tgggatccgc
     4261 gcttcggctg gagctgggtc gacaccgcgc cgtggggctg ggcgccgtat cactacggcc
     4321 ggtgggtgca cctcgatcgc gtctgggcgt gggctccggg gccggtcatc gtgcgcccgg
     4381 tgtacgcgcc ggcgctcgtc gccttcttcg gcgcgcccgg cgtgcgcgtg gccatcgggg
     4441 caccgttcgt gagctgggtg gcgctcggct ggggcgagcc gtgcgtgccg tggtggggcc
     4501 gcccgggctt cgtcggccgc gcctggtggg gcggctgggg cggcccccgg gtcgtcaaca
     4561 acgtggtggt caaccgcacc accgtcgtga acgtgcggaa catcacggtc taccggaacg
     4621 tgggcgtgca gaacgcggtg gtcgcggtgc ggccggatcg cttcgggcgc ggcccggtcg
     4681 gcgacgcccg catccgccag gtggacacgc ggcggctcga gccggtccgc ggggccgtcg
     4741 accttcgccc ggatcgggcg agcttcgcgg cggcgagcgg ccggggcgtc cggccgccgg
     4801 agagcgtgct ggcgcgcccg acggtggcca cccgcgcgcc ggtgacgcgc gccctgccgc
     4861 acgaggccga gggccgcggg ccggcgcccc gcatcgtgcc ggccccgcat gcggcggagc
     4921 ccggcgcggc gagcgcgcgc ccgccattcg gggagagccg gctggagcgc gcgcgcccgc
     4981 ctcaggcccc gcgcttcgac accaaccgcc ggccggacgc gacaccgccg gccacgccgc
     5041 cagccgcgcg ccgtcaggac ggcggcccgg acgccgcgcg ggggcgtgga ccggccagcc
     5101 ctcgaacgga agcaccgcgg ggcgcggagg taccgcgtgg tcccgagccg agccgcgcgc
     5161 cccaggccgc cgtcccggcg gaagccccgc gcgtgccgca ggcccaggcg ccacggtcgc
     5221 cgcagccgca ggccccgcgg caatcagaga tccagcgcgg ggcccagccg cccgcgcgcg
     5281 ctccggaggc ggccgcgccg cgccgggcgc ccgatgcggc gcgcgcgccg cgtccagaag
     5341 gtcctggcgc gcggggggcc gagccgcgcc ccgcggcccc gaccccgcga gctgagacgc
     5401 cgcgccaggc gccgccgcgc gcgctgccgg gtgagcccgc caaccggctc tacccgggac
     5461 gcgtggagcg cccgtcgtcg cgcggaccgg agacgcggcc ccaggcgcgc ccgcggcccg
     5521 cccccgagca caacacgggc cgcacgggcg gggcggcgcg cgaggcgcgc tgactcagga
     5581 gccgggcgcc cactcccccg cgagcgagcg cccgagccgc cgcagggcct gcgcctccag
     5641 ctggcgggcg cgctcgcgcg tcacgcggaa gcgcgcgccg acctcctcga gggtgagcgt
     5701 ctcctccccg tccaggccga agcgcaagcg gaggacttcc cgctcgcggg gcggcaggct
     5761 ctcgatggcg atcccgagct ggtgcgagcg gtcggcgcgg acgatcgtct cctccggcga
     5821 cggggtgacg cgatcgcgca cgaactcgcc gagcgtcgag tcctgaccga tcggcgcgtc
     5881 gagcgagacc ggggtcgccg cgctcagatc gaccaggcgc accgtcttct cggagacccc
     5941 ggtccgctgg gccagctcct cgacgctcgg ctcgcgctcg tgctcggcct ggaaggcgtt
     6001 ccgctcccgc atgagccggt gcatgctctc gatgatgtgg acgggcatcc ggatcgtccg
     6061 ggcctggtcg gcgagggcgc gcccgaccgc ctgacggatc caccacgtcg cgtaggtgga
     6121 gaacttgaag ccgcgatggt agtcgaagcg ctcgaccgcc ttcatcagcc cgatgttgcc
     6181 ttcctggacg agatcgagca gggtgagcga cccacccgcg taccgccggg cgatcgacac
     6241 cacgagccgt agattgggct cgacgagcgc gcgcttggcc tcgcggagcg cgcggtcgct
     6301 ctcctcgacc tcgcggagcg cgggcctcca gcgcgccggg atcggtccat tggcctgccg
     6361 cagctcgccg accaggtcgt ccaccacgtc cggccgcagc ggcaggcgct cgattaggcg
     6421 ccccgcgctc cggcgcgcga tcgcggaatg cgtcagacgc gggcgcatcc ggcgcaggcg
     6481 cgcgagaatc tcgaagatcc gatcccgctg ccggacggtg agcgacgctc cgcccggcag
     6541 cagcaggagc gcgtcgagcg accggtcgcc ccggcccacc tcgcgcgtcg ccgcgagcag
     6601 ggcgtcgatc ccggcgggga gaccggccag cgcgcggcgg cgccgggccc ggcagatctc
     6661 gatccaccgc ccccgctccc gctcctgggc cgcggtgagg cgatcgaccg cgccgatctc
     6721 ccggaagtag agcgccaccg ggttggcgcg cgcgatcgcg acggccgacc gccggccgtg
     6781 gctcgcgaga ccgttcgcca tcgcgcgcgc tcccggcccg gctacgacgt cagcgccgcg
     6841 tagaggctgt tgccgtcccg gtgcagcagg accaccaccg gagcgccctt cgcgtgcttg
     6901 gccagcagtc gcttcatgtc ggccgcgctg gccaccggct ggtgatccag ctcggtgatg
     6961 acgtcgcccg gacggagccc cgccttctcg gccgggctcg agtcgcgcac gccgcgcacc
     7021 accaccccgc gtgcatccgc aagccccagc tggcggccca cgtcgggcgt cagcgtctcc
     7081 accgtgaggc cgagcggctc cttgccttcg tcgcgctggg cgagcgccgg ctgatccggc
     7141 tcgtccatcc gggcgatccg ggtctggagg gccatggtct tgccctcgcg gacgacggtg
     7201 agcgggacgt cgcgcccgac cgcggtggcg gccaccagct tggagagatc gccgggctgg
     7261 ggcagcttgt gaccgtcgaa ctcggtgatc acgtcaccgg ccttgacccc ggcccgcgcc
     7321 gccggcgagt cgggctggac cgacgccacc agcgcgccgc cgcccggcgg cacgttgaag
     7381 cccttggcca gctccggggt gagcggctgg atcatcacgc cgagccagcc ccgcgtcacc
     7441 ttgccggtgg cggcgagctg ggtcaccacc gacttggcct ggttgatcgg gatcgcgaaa
     7501 ccgatgccga ccgagccgcc gctctggctg aagatggcgg cgttgatgcc gaccgcctgc
     7561 ccgcgcgcgt tgatgagcgg gcccccgctg ttgccgggat tgatggaggc gtcggtctgg
     7621 atgaagtcgt cgtagggccc ctgcccgatg acgcggccgg tggcgctgac gatgccggtg
     7681 gtgacggtgc gctcgagacc gaaggggttg ccgatggcca tcaccggctc gccaacctgc
     7741 agcgcggccg agtcgccgag cgggatgacc ggcaggttcg tcgcctcgac cttgaggagg
     7801 gcgaggtcgg tgcgcgggtc acgacccacc accttgccgg gcagctcgcg cccgtcgtcg
     7861 atcttgacct ggatctcggt ggcgccgtcc accacgtggt tgttggtcat gatgtagccg
     7921 gccggattga tgacgaagcc cgagcccatg ctgcgcacgg tcttgcgccc cggctggccg
     7981 aactgcttga agaactgctg gaacggatcg tcgttgccga acgggctctg catcgacgtc
     8041 ccgtcctgga cccgcttggt gctgatgttg accaccgccg gcttgagggc ccgggtcagc
     8101 tccacccagt tgggcgcggg cacggtggcg gcggcggtga ccgataccgg ccgctcgctc
     8161 cagatcggcg ccggcgcgga gccggtgaat cccaggtgcg gggcgagggc cacgaggccc
     8221 accaccgcgg ccacgatcag cgccgtccag gcggcgcgat aacgcatcgt cagtcgattc
     8281 atgatcgtct ctccttgtgt tggttgatct acggggccgc ctgctcccgg gcggcccgga
     8341 gctgttcacg ggccagcgcc cccagggtgt cgcccccggc caggcgcatc gcgatccggt
     8401 actcgcgctc catcccaacg gtgtcgcccg cccgccggag cgcatcggcg agcagcagtc
     8461 ggatatcggg ggaggtgggc ttcatgtcga cggcccggcg caggtgcgcg atcgcctccg
     8521 gcgtccggcc cgcgtcgagg aacaaggtgg ccaggcccgt ctcgtaccgc gcgtcgcgcg
     8581 gggccagccg gaccgcgtcc tcggcgtggc tcagcgcggc cgcgtcctgt ccgagaccgg
     8641 cgtaggcgag gccgagatag tagtgcgcgc gcgcgtcctg cggcgcggcc gccaccgcct
     8701 ggacgagctg gcggaccgcg gggagatact cgccgtccga cacgagggtg gccccgctct
     8761 cgacgaggcc gtgggcgcgc caccaggcgg gcccgctcgc gagcgcggcc agcgcggccg
     8821 cgagcactcc gcccatcgcg agacggccga tccagggcga gcgggagatc cacgtctgcg
     8881 gcatgcctgg aacgtagccc gtccacatga gggacgggtg aagccgcgat gagaaacttc
     8941 ttagcggggg gagtgggcgg gggcgggctc ggcggacgcg gtgacgagcg tcaggttccc
     9001 gtggcacttc gggcagtcga agctgcggcc gtcgacgaat cgcccgcccg gccgcagctc
     9061 ctgcaccgcg ccgcaccggg ggcaggcccc gcgcaccagc agcaggcggc gatcttcccg
     9121 cgcgcgcttg accgccatga cgatgcccgc caccacgaag gtcgggacca ggacgaagtg
     9181 ggccaccggg atgaacaccg aggccagccc gagcccccag aacatcccga gcccggtgag
     9241 ggtccgaacg aagcgctgtc ccggcgcctg ctcgaggatg accgcctggg ccggctgcga
     9301 tcgatgcccg aaggcggtga gctgcgcggg aacctcggtc tcggtcgaca tgtgatcgtc
     9361 tcctctccct ccatttaccc taccaggagc ggatgagagc ccgatgaggc tcggatgaga
     9421 gtcacgcggc cgtcgagggc aggcggaccg tgaagcggct gcccgacccg ggccggctct
     9481 cgacggtgat ggcgcccccg tgggcgatca cgatcgagcg cgcgatggcg aggcccaggc
     9541 ccgcgccgcc ggtgtctcgc gcgcgggccg cgtcgaggcg aagaaagggc tcgaagatcc
     9601 gctccagatc gtccgcttcg ataccgatgc cgctgtccgc gatcgtcacc tcggccacgc
     9661 cgtcgccccg cccgagcccg atctccacct tgccgcccga cggtgtgtac ttgatcgcgt
     9721 tctccaccag attgaggagc gcgcggcgca gcgcggccgc gtccccggtc acccgggcgg
     9781 gcaccgtcgc gtcgacgcgc acgctcaccc ccgcggcccg cccgatgcgc gcgcccacct
     9841 cgaagacctc gagcacgagc ggctccaggt cgacgggcgc ccgggcgccc tcggggccgg
     9901 cgagcgagcg cgagagcagc aacaggtcct ccgcgagccg gatcaggcgc tcgacctcct
     9961 cgaggctcga ggcgagcacg cgccggtact cgtcgggcga gcgctccatc cggagcgcca
    10021 cctcgatgcc gccgcgcagc acggtgagcg gggtgcgcag ctcgtgggcc gcgtccgccg
    10081 cgaagcggcg ggtctgcccg aacgcgtcgt cgagccgcgc cagcatcccg ttcagggtct
    10141 cggcgagcgc gtcgagctcg tccccgctct cccgccgctc cacacgccgg ctcaggtcct
    10201 cggcggtgat gcggcgggcg gtgcgcgcca tctcgtccac cgggcgcagg gccgcgcggg
    10261 cgatgatggc cccgccgagc gcggccagcc ccacccccag cgggatgagc acgatcaggg
    10321 tgtccaagta gcggtcgcga accgcctggg tgcgccgcag cgccatgccc acctggatca
    10381 tctcggtgac gcggccgtcg cgcaccaccg gcagggtgag gaggcggacc ggctcgaggc
    10441 cgcccaccac caccgtctcg atcgtgcgca gcccgcgggc ggcccgggcg cgggcctggg
    10501 gcgacagcgg cagcacgtcg gcccggcggc ccgcgagggg ggcgtgcggc cgcccctcgg
    10561 gatcgaggag ctggacgaac ttgtcgtaca gctcgggtcc caggagctgg cgcaggagcg
    10621 catcggagtc gcccgacgcc tgctcggagg agccggtgtc cttgatcacc tgcgcgacgg
    10681 tgaggagcga cgcgtcgaga tcttgctgga ggccgaagct cagccaggaa taggagatcg
    10741 cggcgatcgt caccaggatg gcgagcagga cgccgctgta ccacagggtg agcttggtgc
    10801 ggatcgagag cggcagccgg aggcctctca cgactcggcg ctcagcatgt agccggcgcc
    10861 gcggacggtc tgcagcaggc gggcctcccc ctccgcgtcg atcttccggc gcaggtagcc
    10921 cacgtagacg tcgaccacgt tgctctccgg atcgaagtcc atgccccaga cgtgctgggt
    10981 gatcatgggc cgggtcagca cgcggtccgc gttgcgcagg aagtactcga gcagggcgta
    11041 ctcgcgcgcg gtgagcgtga tgcggcggcc accccgcgtg accgcgcggg tcgcggggtc
    11101 gagggagaga tccgccacct gcaggcgcgg ctcggcccgc cccgggctgc gccgcaggag
    11161 ggcccgcacc cgcgccacga actcctcgaa ggcgaacggc ttggtcagat agtcgtccgc
    11221 cccgaggtcg agcccggcga ccttgtcggc gaccccgtcg cgcgcggtca ggagcaggac
    11281 cggcgtctgg acgcgccgct cgcgcagggt ccggagcacg tcgaagccgt cgcgcttggg
    11341 cagcatcacg tcgaggacca ccaggtcgta ggcgggatcc tccgcgatcg cgtcgagcgc
    11401 ggcggccccg tcggacgcga ccgcgaccgc gtggccttcc tccaccagcc cctggcggat
    11461 gaacgcggcg accttgcgct cgtcttcgac cacgaggatc cgcatgtcgg atgtagaatc
    11521 gcaccgggtc ccgggagccg caagcggcgc gggggaccgc ggcctgggaa tgctcgggtc
    11581 agcccgagct cgtttccaac tagagg
//
LOCUS       DATMCV010000617         8959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_231630, whole genome shotgun sequence.
ACCESSION   DATMCV010000617 DATMCV010000000
VERSION     DATMCV010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231630"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VNU03_14225"
     CDS             complement(<1..1529)
                     /locus_tag="VNU03_14225"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWP75675.1"
                     /translation="MLSQPFQDAIRQRLSIPVHEGPDKTTTLEQALRQHVPPGATVYL
                     GAAHGRPNPAVRELVRQWWGKTPGFTLATVGLGSPWTALVHGGLVRRVITTFMGEGYP
                     FPTPQALISRAVLEGRLEVSNWSMLTFPLRLLAGAMGVPFLPTRSLLGSTMEEDNARS
                     GDLIAADDPFGAEGRVAYVRALVPDVALLHGWVADRAGNVITASPMNENMYGAMAARG
                     GAVVTVEKIVSTDFIRRHADLVRLPSQYVRAVVETPHGNHPGGMYGMNVSELEGYAED
                     MEWILECRRAFRKPDTADAWIREWMLDLPDHAAYLSKLGYARIMETKGRAHTDAWVSE
                     LDTLAPKFSTSATATPSEWMVVAASRMLAEKVRARGYKSFLAGVGNSNLAAWLAAYQL
                     KQSGAEVELMAETGMVGYLPRPAEPFVFSFRNFPSCKMLTDILHVMGIFMGGRHNQCM
                     GSLAAGQIDKHGNINSTIMPGVTYITGSGGANDITSSSREVVVSLQQSPRRFVDKVPY
                     IT"
     gene            complement(1578..1934)
                     /locus_tag="VNU03_14230"
     CDS             complement(1578..1934)
                     /locus_tag="VNU03_14230"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HWP75676.1"
                     /translation="MKELLLALPRLARMLVSLAGDRDVPTAAKVVLAAVAVYLVSPID
                     LIPDFIPWVGYLDDLVIAAVVVDGVINFIERPLLLRYWPGSPASLDRIGAVASRLARW
                     VPSRVKSRIFGARRAA"
     gene            complement(1962..3953)
                     /locus_tag="VNU03_14235"
     CDS             complement(1962..3953)
                     /locus_tag="VNU03_14235"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWP75677.1"
                     /translation="MLPSHQWYGIIPGWLLFYALIAAAAVLFGRRAFYLLRLLLKGKP
                     LARWDHIPARVGRVIVYVFGQARLLANDFWPGLMHATIFWGFVILTLGTVEFFGKAVT
                     ESFFLPAMSDTAPYLIIEDVFSVLVILAVGYAMFRRLVTRPRRLNLSTEANVILLLIL
                     GLMVTDLVADAGRIVLAPAPSDHWQFAGQALARVLAALPPAAVTVIFHSAWWLHAATL
                     LGFLVLLPYSKHLHILVSPLNVFFSPLEPKGQARNVDLENSETFGVGSLADLAWKDLF
                     DTYNCTECGRCTSRCPANMSGKELDPKWLILHLRERLFEEAPALLGQAGAAAPEKALV
                     GDVIHDNVLWACTTCRWCVDACPVFIEHVPKIVDMRRWLVLTESRFPAELQPAFRNLE
                     TNGNPWQMSWETRAEWAKDLGVRQMADVHEAEYLYWVGCYGSFDERNKKVARALVRVL
                     QAAGVDFAILGNEEKCTGEPARRLGHEYLYQTLAQGNIETLKRYKFQTIVTACPHCFN
                     TIRNEYPQFDGHFRVVHHTQLMDELLKSGRLQPAQRDNERITYHDACNLGRYNDIYDE
                     PRRVLAGVTRGSLVEMKMNRSKGFCCGGGGGRAWLEEHEGRRVNQLRVEQAMEVKPDI
                     LASACPFCLTMFEDGVKAKEVGDTIKTRDIAELLADRLE"
     gene            complement(4002..4967)
                     /gene="meaB"
                     /locus_tag="VNU03_14240"
     CDS             complement(4002..4967)
                     /gene="meaB"
                     /locus_tag="VNU03_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011013089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWP75678.1"
                     /translation="MTDLVRRMLDGDRLALARLITRVENRASDVPEIMRAVHARTGRA
                     YVLGITGPPGAGKSTLVDRVTARLRAEKQPVGVIAVDPSSPFTGGAVLGDRIRMQTHT
                     LDPDVFIRSMATRGSLGGLARATGDVIKLMDAFGFPWIIVETVGVGQTELDIIRQVDT
                     TVVALVPESGDSIQAMKAGLMEVADVFVVNKADRDGAHALMAELRFSVHLHYTSGGAP
                     KDADWEVPVLAAQAANDVGIDELIGQVKRHRAVLEQAGALETRRQARRRAELEALLVE
                     EFTAQVTARVQTDAALGATIEAVTAGRLDPYSAVAEILSQTLRRP"
     gene            complement(4982..5449)
                     /gene="rimI"
                     /locus_tag="VNU03_14245"
     CDS             complement(4982..5449)
                     /gene="rimI"
                     /locus_tag="VNU03_14245"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWP75679.1"
                     /translation="MTLEDLDDVLVIERASFQTPWSRGAFRYELTQNRVARCLVARRG
                     RRLLGYLCLWEIGHEIHVTNLAAHPDLRRRGVARALLGAVLGDAKTRAVSLAFLEVRP
                     TNTEALGLYESFGFRVIGRRKGYYFDTGEDALVMEARLSAEPSESAASPRPGA"
     gene            complement(5490..6173)
                     /gene="tsaB"
                     /locus_tag="VNU03_14250"
     CDS             complement(5490..6173)
                     /gene="tsaB"
                     /locus_tag="VNU03_14250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWP75680.1"
                     /translation="MRLLALETATLAGGAALLDDGRLVGESRLNIALTHSERLMAVVD
                     RLLQDCGWEMSTLRGLAVSIGPGSFTGLRVGAATAKGLALALDLPIAPVPTLDALAAT
                     LPFADAPVCPLLDARKGEIYCSLYRWSGAEMIRVWDYLALPPAEAAARLVAPVIVVGD
                     GVAACRPHMARLGAGLREAPPAQSLPSPAVVGALGHAMLRAGGGVPAERLQPLYLRPS
                     EAELKIRHA"
     gene            6300..6947
                     /locus_tag="VNU03_14255"
     CDS             6300..6947
                     /locus_tag="VNU03_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWP75681.1"
                     /translation="MDAEVREKIHLEILKRTGAYHANDHVLLPSGHHTSEYIEKTLVT
                     TEASFTEGLGAVIAKHFAQWPIDVVLSTGPGALILSHCVARAHPSRPTLAYGTKGVSA
                     GKRRVTLPVEFQRFIRTGTKVLIVEDLVSSGTTVRLLSQLVEDLGGQVIGIGALWRRT
                     KKAEIGGKEIFSLVSRDFPTYAPETCPLCKKGVALNQEYVKRREHRKPSSAHEKT"
     gene            7735..8127
                     /locus_tag="VNU03_14260"
                     /pseudo
     CDS             7735..8127
                     /locus_tag="VNU03_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(8135..8641)
                     /gene="nrdR"
                     /locus_tag="VNU03_14265"
     CDS             complement(8135..8641)
                     /gene="nrdR"
                     /locus_tag="VNU03_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWP75682.1"
                     /translation="MKCPFCGHTEDRVVDSRVGRDGEFIRRRRACLKCHRRYTTYEYI
                     EDVLPHVVKRDGRREPFDRQKLRGSILKSCEKRSVGVQAVDDVVADIEAKLHDRAEKE
                     ITSLELGEIVMEHLQKLDQVAYVRFASVYRQFKDISQFMDEVKHLIKDDKPAKPASSK
                     GPKASAKR"
     gene            complement(8638..>8959)
                     /gene="glyA"
                     /locus_tag="VNU03_14270"
     CDS             complement(8638..>8959)
                     /gene="glyA"
                     /locus_tag="VNU03_14270"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999706.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWP75683.1"
                     /translation="THLILVDLTTRNVTGKDAQETLDRAWITVNKNGIPFDTKGPMVT
                     SGIRIGTPAVTTRGMKEPEMARIADFIDRVLQRAGDGAVEAAVRGQVQELTSQFPLYA
                     GRLK"
BASE COUNT         1315 a         3122 c         3197 g         1325 t
ORIGIN      
        1 gtgatgtacg gcaccttgtc cacgaagcgg cgcggcgact gctgcaggga caccacgacc
       61 tcgcgcgagg acgaggtgat gtcgttggcc ccgcccgagc cggtgatgta ggtgacgccg
      121 ggcatgatgg tcgagttgat gttgccgtgc ttgtcgatct ggccggccgc gagcgagccc
      181 atgcactggt tgtggcgacc gcccatgaag atgcccatca cgtgcaggat gtcggtcagc
      241 atcttgcacg acgggaagtt gcggaacgag aacacgaagg gctcggccgg gcgcggcagg
      301 tagccgacca tgccggtctc cgccatcagc tcgacctccg cgccggattg cttcagctgg
      361 tacgcggcca gccacgcggc caggttggag ttgcccaccc cggccaggaa gctcttgtag
      421 ccgcgggcac gcaccttctc ggccagcatg cgggaggccg ccaccaccat ccactcgctc
      481 ggcgtcgcgg tcgcgctcgt ggagaacttc ggcgcgagcg tgtcgagctc cgagacccag
      541 gcatcggtat gggcccgccc cttcgtctcc atgatgcggg cgtagccgag cttcgagagg
      601 taggcggcgt ggtcgggcag gtccagcatc cactcccgga tccacgcgtc cgcggtgtcg
      661 ggcttgcgga aggcccggcg gcactcgagg atccactcca tgtcctcggc gtagccctcg
      721 agctccgaca cgttcatgcc gtacatgccg cccgggtggt tgccgtgcgg agtctccacc
      781 accgcgcgga cgtactgcga aggcagccgc accaggtcgg cgtggcggcg gatgaagtcg
      841 gtggagacga tcttctcgac cgtcaccacc gccccgcccc gggccgccat cgccccgtac
      901 atgttctcgt tcatcggcga ggcggtgatc acgttgccgg ctcgatcggc cacccagccg
      961 tggagcagcg ccacgtccgg aacgagggcc cgcacgtagg ccacccgacc ctcggccccg
     1021 aacggatcgt cggccgcgat caggtccccg gaccgggcgt tgtcctcctc catggtcgag
     1081 cccagcagcg agcgggtggg caggaacggg acgcccatcg cgccggccag caggcgcagc
     1141 ggaaaggtca gcatcgacca gttcgagacc tcgaggcggc cctcgaggac ggcgcgcgag
     1201 atcagcgcct gaggcgtcgg gaacggatac ccctcgccca tgaacgtggt gatgacccgg
     1261 cgcacgaggc cgccatggac cagcgcggtc cagggtgagc cgaggccgac cgtggcgagg
     1321 gtgaagccgg gggtcttgcc ccaccactgg cgcaccagct cgcgcaccgc ggggttcggg
     1381 cggccgtggg cggcgccgag gtagaccgtc gcgccgggcg ggacgtgctg gcggagcgcc
     1441 tgctcgagcg tcgtggtctt gtcggggccc tcgtggacgg gaatcgagag gcgctggcgg
     1501 atggcgtcct ggaatggctg gctcagcatg gggacccctc accctgccct ctccccaggg
     1561 gggagagggg gagatggtca ggcggcacgg cgggcgccga agatgcggct cttcacccgc
     1621 gacgggaccc agcgggccag gcggctcgcc accgccccga tgcgatcgag cgaggccggc
     1681 gagcccggcc agtagcggag caggagcggc cgctcgatga agttgatcac accgtcgacg
     1741 accaccgcgg cgatgacgag gtcgtcgagg tagccgaccc acgggatgaa gtccgggatc
     1801 aggtcgatcg ggctcacgag gtagaccgcc accgcggcca gcacgacctt ggccgcggtc
     1861 ggcacgtcgc ggtcgccggc cagggagacc agcatccggg ccaggcgcgg cagggcgagc
     1921 agcagctctt tcatggtatg ggcatggcgc aggcgccccc gctactccag gcggtcggcg
     1981 aggagctcgg cgatgtcgcg ggtcttgatg gtgtcgccga cctccttcgc cttcaccccg
     2041 tcctcgaaca tcgtgaggca gaacggacag gcggaggcca ggatgtcggg cttcacctcc
     2101 atcgcctgct ccacccgcag ctggttcacc cgccgcccct cgtgctcctc caaccacgcg
     2161 cgcccgcccc cgcccccgca acagaagccc ttggagcgat tcatcttcat ctccaccagg
     2221 ctgccgcgcg tgacgccggc gagcacccgg cgcggctcgt cgtagatgtc gttgtagcgg
     2281 ccgaggttgc acgcgtcgtg ataggtgatc cgctcgttgt cgcgctgcgc gggctgcagg
     2341 cgcccggact tcagcagctc gtccatcagc tgggtgtggt gcaccacccg gaagtggccg
     2401 tcgaactgcg ggtactcgtt gcggatggtg ttgaagcagt gcgggcacgc ggtcacgatg
     2461 gtctggaact tataccgctt gagggtctcg atgttgccct gggccagggt ctggtagagg
     2521 tactcgtggc ccaggcgccg ggccggctcg ccggtgcact tctcctcgtt gcccaggatc
     2581 gcgaagtcca cccccgccgc ctgcagcacc cgcaccaggg cgcgggccac cttcttgttg
     2641 cgctcgtcga aggagccgta gcagccgacc cagtagaggt actcggcctc gtggacgtcc
     2701 gccatctgcc gcacgcccag gtccttggcc cactccgcgc gcgtctccca tgacatctgc
     2761 cacgggttgc cgttggtctc gagattgcgg aacgcgggct gcagctcggc cgggaagcgc
     2821 gactcggtga gcaccagcca gcggcgcatg tcgacgatct tcggcacgtg ctcgatgaag
     2881 accgggcagg cgtccacgca ccagcggcag gtggtgcagg cccacagcac gttgtcgtgg
     2941 atgacgtcgc cgacgagggc cttctccggc gcggcggccc ccgcctgccc cagcagcgcg
     3001 ggcgcctcct cgaagagccg ctcccggagg tgcaggatga gccacttcgg gtccagctcc
     3061 ttgccgctca tgttggccgg gcagcgcgag gtgcagcggc cacactcggt gcagttgtag
     3121 gtgtcgaaga gatccttcca ggccaggtcg gcgaggctgc cgaccccgaa ggtctcggag
     3181 ttctcgagat ccacgttgcg ggcctggccc ttgggctcga gcggggagaa gaacacgttg
     3241 agcggcgaga cgaggatgtg caggtgcttg gagtacggca gcaggaccag gaagcccagc
     3301 agggtggccg cgtgcagcca ccaggcggag tggaagatca cggtgaccgc ggccgggggc
     3361 agcgcggcga ggacgcgggc gagggcctgc cccgcgaact gccagtggtc cgaaggggcg
     3421 ggcgcgagca cgatgcggcc ggcgtccgcc accaggtccg tgaccatgag gcccaggatc
     3481 agcaggagga tcacgttggc ctcggtggag aggttcaggc gccggggccg ggtgacgagc
     3541 cggcggaaca tcgcgtagcc caccgccagg atcaccagca cgctgaacac gtcctcgatg
     3601 atcaggtagg gcgcggtgtc cgacatcgcc ggcaggaaga acgactcggt gaccgccttg
     3661 ccgaagaact cgacggtgcc gagggtcagg atcacgaagc cccagaagat ggtggcgtgc
     3721 atcaggcccg gccagaagtc gttggcgagg agccgcgcct gcccgaacac gtacacgatc
     3781 acccggccca cgcgcgcggg gatgtggtcc cagcgggcga gcggcttgcc cttcagcaac
     3841 aggcgcagca ggtagaaggc ccggcgtccg aagagcaccg cggcggccgc gatcagcgcg
     3901 tagaagagca gccagccggg gatgattccg taccactgat gagagggcag catcagggac
     3961 gcctcatggg ttcgatagtg tggagtgcgt ccccggaggt gtcaagggcg ccggagcgtc
     4021 tgggagagga tctcggccac cgcggagtac ggatcgaggc ggcccgcggt caccgcctcg
     4081 atcgtggcgc cgagcgcggc gtcggtctgc acccgcgcgg tgacctgcgc ggtgaactcc
     4141 tccaccagca gcgcctccag ctcggcgcgc cgccgcgcct gccggcgcgt ctcgagcgcg
     4201 cccgcctgct ccagcaccgc gcggtggcgc ttcacctggc cgatcagctc gtcgatcccc
     4261 acgtcgttgg cggcctgggc ggccagcacc ggcacctccc agtcggcgtc cttgggggcg
     4321 ccgccgctcg tgtagtgcag gtggacgctg aagcgcagct ccgccatcag ggcgtgcgcc
     4381 ccgtcgcggt cggccttgtt gaccacgaag acgtccgcca cctccatgag ccccgccttc
     4441 atggcctgga tggagtcgcc cgactccggc accagggcca ccaccgtggt gtcgacctgt
     4501 cggatgatgt cgagctcggt ctgccccacc ccgaccgtct cgacgatgat ccacgggaag
     4561 ccgaaggcgt ccatcagctt gatgacgtcg ccggtggcgc gggccaggcc gcccaggctg
     4621 ccgcgcgtcg ccatgctgcg gatgaacacg tcgggatcca gggtgtgggt ctgcatgcgg
     4681 atccggtcgc ccagcaccgc gccgccggtg aacgggctcg acgggtcgac cgcgatcacg
     4741 cccaccggct gcttctccgc gcgcagccga gcggtcaccc ggtccaccag cgtggacttg
     4801 cccgcgccgg gcggcccggt gatgccgagc acgtaggcgc ggccggtgcg cgcgtggacc
     4861 gcgcgcatga tctcgggcac gtcggacgcg cggttctcga cccgggtgat caggcgcgcg
     4921 agggccagac ggtcgccgtc cagcatgcga cggacgagat cggtcaccgg cggtgccgcg
     4981 ctcaggcgcc cggccgcggg gaggcggcgg actcggacgg ctccgcgctc agccgggcct
     5041 ccatcaccag ggcgtcctcg ccggtgtcga agtagtagcc cttgcggcga ccgatcaccc
     5101 ggaacccgaa gctctcgtag agcccgaggg cctcggtatt ggtggggcgc acctcgagga
     5161 aggcgaggct caccgcgcgg gtcttcgcgt cgcccagcac cgcgcccagg agcgcgcggg
     5221 ccaccccgcg gcggcgcagg tccgggtgcg cggccaggtt ggtgacgtgg atctcgtggc
     5281 cgatctccca gaggcagagg tagccgagca gccgccggcc ccggcgcgcc accaggcagc
     5341 gggcgacccg gttctgggtc agctcgtagc ggaacgcgcc gcgcgaccac ggggtctgga
     5401 acgacgcgcg ctcgatgacg agcacatcgt ccaggtcttc gagcgtcatc gggtcgatgc
     5461 tcagcggatc ttcatcgcgc aacatggggt cacgcatgcc ggatcttcag ctccgcttcc
     5521 gaggggcgga gatagagcgg ctggaggcgc tcggccggga cgccgccgcc cgcccgcagc
     5581 atcgcgtggc cgagggcgcc gaccaccgcg ggcgagggca gcgactgggc cggcggggcc
     5641 tcgcgcaggc ccgcgccgag ccgggccatg tggggccggc aggccgcgac gccgtcgccc
     5701 accacgatga ccggcgccac gaggcgcgcg gccgcctccg cgggaggcag cgcgaggtag
     5761 tcccacaccc ggatcatctc ggcgccgctc caccggtaga gcgagcagta gatctcgccc
     5821 ttgcgcgcgt cgagcagcgg gcacactggc gcgtcggcga acggcagcgt ggcggcgagg
     5881 gcgtcgagcg tcgggaccgg cgcgatgggc aggtcgaggg ccagggccag gcccttggcg
     5941 gtggccgcgc ccacgcgcag gccggtgaag gagccgggcc cgatggacac cgcgaggccg
     6001 cgcagggtcg acatctccca cccgcagtcc tggagcagcc ggtccaccac cgccatgagc
     6061 cgctccgagt gcgtgagcgc gatgttgagc cggctctcgc cgaccaggcg gccgtcgtcg
     6121 agcagcgcgg ctccgcccgc gagcgtggcg gtctcgagcg ccagcagcct catgcgcggc
     6181 agtctagcat ggcgcggcgg ccgcacgcgg tcggccactt tcgagttgac aactcatcgc
     6241 gtcggcccgc attcttgatt cgactgatgg cccccccgcc gcgacaaacc gacagccaga
     6301 tggacgcgga ggttcgcgag aagattcatc tcgagatcct caagcgaacc ggggcctatc
     6361 acgcgaacga ccacgtgctg ctgccctccg gccatcacac cagcgagtac atcgagaaga
     6421 ccctcgtcac caccgaggcc tccttcaccg aggggctcgg ggcggtcatc gccaagcact
     6481 tcgcccagtg gcccatcgac gtggtgctct cgaccgggcc cggggccctg atcctctccc
     6541 actgcgtggc ccgcgcgcat ccgtcgcggc ccaccctggc ctacggcacc aagggggtca
     6601 gcgcgggcaa gcggcgggtg accctgccgg tggagttcca gcgcttcatc cgcaccggca
     6661 ccaaggtgct gatcgtcgag gacctggtca gctccggcac cacggtgcgg ctcctgtccc
     6721 agctcgtgga ggatctcggc ggccaggtga tcggcatcgg cgcgctgtgg cgccgcacca
     6781 agaaggcgga gatcggcggc aaggagatct tcagcctggt cagccgcgac ttcccgacgt
     6841 acgcgcccga gacctgtccg ctctgcaaga agggcgtggc gctcaaccag gagtacgtga
     6901 agcgccgcga gcatcgcaag ccgtcgtccg cccacgagaa gacctgacgt ctcgccccca
     6961 agatatgggg gctgccgagc gccgaccacc ctctatccgg tattttcccc ttgacacgtt
     7021 ttttgccctc ccctattgtc cggacagatc gaccgcgtga cgcgcgggga tcgggagggg
     7081 ggtgacatca catggcgaag aagaaggccg cgaagaagaa aaagaagaag taacgcgcca
     7141 ctcgtggcgc tcggccctgt cggccccatc ccggcagggc cgttccatct ccgagcccgc
     7201 ccctacccgg aaccgaagcc gtgacccacc gacgtgccta cgcgctgctc ctcggcgtcg
     7261 ccctgctctg ggccgggaac tatccgctcg cgaagatcgg cctgcatgag ctcggccctg
     7321 tcacgatgtc ggccctgcgc gccctcatcg cggcgccgct gctggtgctg gtttcgcggc
     7381 tgctcgaggg gccgctgccg cccctggcgc gccgcgacct catcgcggtg gtggtgctgg
     7441 cggtcagcgg cttcgtgggc aactccgcgc tgtggtacgt ggggctgcgg ttgacgagtc
     7501 cggccaacgc ggggatcctg ggcgcggcct ccccggtggt ggtggccctc gcggcggcgg
     7561 cgtggctgcg cgagcgcgtg tcgccggtca acctggccgg catcggcctc accctcgtgg
     7621 cggtgctgct gaccatcgcg cagggatcgc cccgcaccct gctgacgctc tccgtcaacc
     7681 gcggcgacgt gatcatcctg gtctcgcaga gcctgtgggt ggtctacacg ctacagccgc
     7741 gccaacctct ccacgttctc gccgctccag atgctggccg gcgcgcacgt ggtcgcggcg
     7801 ggcctgctgc tgccgatggc cctcgtggag cggccgtggc aggcgttcgc gcacgcctcg
     7861 tgggtgggct tctcggtcgt gctctacagc gccttcctgg ggacgccggc ccacatcgcc
     7921 ttctaccagg cggtgcgcac cgtggggccg agccgcgccg cggtcttcat gaacctgatc
     7981 ccgttcatgg tgctggggct gtcgtggctg atgctgggcg agccgatcca ctggtaccac
     8041 tgggtgggcg cgatcggggt gatcgggggc gtggtgctga cgacgcgccg ctcgcacccg
     8101 atccccggcc gggggagggc cggatgaggg gcggctaccg cttggccgag gccttcggcc
     8161 ccttgctcga ggccggcttg gccggcttgt cgtccttgat caggtgcttc acctcgtcca
     8221 tgaactggga gatgtccttg aactggcgat acaccgaggc gaaccgcacg taggcgacct
     8281 ggtcgagctt ctgcaggtgc tccatcacga tctcgcccag ctcgaggctc gtgatctcct
     8341 tctcggcccg gtcgtggagc ttggcctcga tgtcggccac cacgtcgtcg accgcctgca
     8401 ccccgaccga gcgcttctcg caggacttga ggatggagcc acggagcttc tgccggtcga
     8461 agggctcgcg gcgcccgtcg cgcttgacca cgtggggcag cacgtcctcg atgtactcgt
     8521 acgtggtgta gcggcggtgg cacttgaggc acgcccgccg gcggcggatg aactccccgt
     8581 cgcgccccac ccgcgagtcc accacgcggt cctcggtgtg gccgcagaac gggcacttca
     8641 cttgaggcgc cccgcgtaga gggggaactg ggaggtcagc tcctgcacct ggccccgcac
     8701 cgccgcctcc accgccccgt cgccggcgcg ctggagcacc cggtcgatga agtccgcgat
     8761 ccgcgccatc tccggctcct tcatgccgcg cgtggtgacc gccggcgtcc cgatgcggat
     8821 gccgctcgtg accatcgggc ccttggtgtc gaaggggatg ccgttcttgt tgacggtgat
     8881 ccaggcccgg tcgagcgtct cctgggcgtc cttgccggtc acgttgcggg tggtgagatc
     8941 caccaggatc aggtgggtg
//
LOCUS       DATMCV010000618         3195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_766730, whole genome shotgun sequence.
ACCESSION   DATMCV010000618 DATMCV010000000
VERSION     DATMCV010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766730"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VNU03_14275"
     CDS             complement(<1..540)
                     /locus_tag="VNU03_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HWP75684.1"
                     /translation="MDQHVKARLTKVLDAEGLDALVATTPENLQYVTGFRSIAHALFR
                     GLELYGVFTRQGVGLVIPFIDSTGVSADGIQVDRIACYGNFFFNYTDQPGPIGTRVRE
                     ITSRPAAGPGEALREVLGAFGVTGRRIAVDEAGVFPATWQRLGEQLAGTTITPAYAHF
                     RIARMVKSPVEVARLEKAAR"
     gene            complement(565..1569)
                     /locus_tag="VNU03_14280"
     CDS             complement(565..1569)
                     /locus_tag="VNU03_14280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP75685.1"
                     /translation="MRFGVHIPTCIEGMMYPVPFAKPSDILPTALLCERLGFDSVWGN
                     DHMTTQRYVQKEFPDPPNFFEPLMTFAYCASRTTRLRFATGIIVLPMRNMPVLAKQVA
                     TLDQLSGGRVILGVGTGAYREEYEALFPDARGARRGTIVDEGMQALRLLFTERRATFR
                     GQAVHFEEVECFPKPVQSPMPIYAGGNHPEVRRRAGQWAQGWMPAVLSPEEITRGIDD
                     IHRAAAAAGRDGSAIDIAPQFAVSIGRTHDEAVSRFRGSQLFQHLESLKKSTLREQTG
                     GFDQRNLIGSPVEISERIRTYQRVGVTTFAGMLFVANTVTEMQEAIELFGQEVLPNFR
                     "
     gene            complement(1580..2731)
                     /locus_tag="VNU03_14285"
     CDS             complement(1580..2731)
                     /locus_tag="VNU03_14285"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75686.1"
                     /translation="MKLFTLLASLALAAIGLAPIPGFAADPVKFGFSAPLTSPIAFIA
                     ERAKLGTEYAVAELNAKGGILGRKVEVSYGDTRLDPNEAINIARRYTLQEKVHAYFGD
                     FTSSGTLAIIDILARANVPQITYTNAIKITRSGYKHVINLKPNSDHLTSAALDYAVKE
                     KKYRKLAVMALDDEYGKGNVDAIDRYLKKFGGAEVVGKEWYRLDAKEFRPLLTKLAGL
                     GPDAIVVTGSLADTALLVKQYGELGLKVPLVGGGGHSSKAFRGIAGSASDGMIVVPAF
                     WPGHFKNKEAVDFEKGFHAKYTALEGDEWSMFAYDAVMIVAGAMERAKSTDGAKVIPE
                     MFKTGPISGAAGTYTITPNGEIDTTVFIGTWGPDGKVQLIRAWSPPKLD"
     gene            complement(2767..>3195)
                     /locus_tag="VNU03_14290"
     CDS             complement(2767..>3195)
                     /locus_tag="VNU03_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP75687.1"
                     /translation="LTVRENLRLGAYRAAAGEIEGRVERVLSAFPALAERLRQMGGTL
                     SGGEQQMLAIARALMSNPTLLILDEPSTGLAPTMVESIFRTIQDLHRRGTMVLLVEQN
                     AHIALELASRAYVLEQGSIVAEGPAAALRQDARVVAAYLG"
BASE COUNT          479 a         1104 c         1091 g          521 t
ORIGIN      
        1 ccgggccgcc ttctcgagtc gggcgacctc caccggactc ttgaccattc tggcaattcg
       61 gaagtgggcg taggccggcg tgatcgtggt gcccgcgagc tgctcgccga ggcgctgcca
      121 cgtggcggga aagacacccg cctcgtcgac ggcgatccgc cgcccggtga ccccgaaggc
      181 ccccagcacc tcgcgcagcg cctcgccggg cccggcggcg ggccggctcg tgatctcgcg
      241 cacccgggtg cctatcgggc ccggctggtc ggtgtagttg aagaagaagt tgccgtagca
      301 ggcgatgcgg tcgacctgga tcccgtcggc cgacacgccg gtcgagtcga tgaacgggat
      361 gacgagcccc accccctggc gcgtgaacac cccgtagagc tcgaggccac ggaagagcgc
      421 gtgggcgatc gagcggaagc cggtgacgta ctgcagattc tcgggggtgg tcgccacgag
      481 ggcgtcgagc ccctcggcgt ccagcacctt cgtgagccgc gccttcacgt gctgatccat
      541 gacgatctcc tcggacgtgt gagcctagcg gaaattggga aggacttcct ggccgaacag
      601 ctcgatggcc tcctgcatct cggtcaccgt gttggccacg aacagcatgc ccgcgaaggt
      661 ggtgacgccc acccgctggt aggtccggat gcgctccgag atctcgaccg ggctgccgat
      721 caggttgcgc tggtcgaagc cgccggtctg ctctcggaga gtcgacttct tgagcgactc
      781 caggtgctgg aagagctgcg agccgcggaa gcggctcacc gcctcgtcgt gggtgcgccc
      841 gatcgacacc gcgaactggg gcgcgatgtc gatcgccgac ccgtcgcggc ccgccgccgc
      901 ggcggcgcgg tggatgtcgt cgatcccccg cgtgatctcc tccggcgaga gcaccgcggg
      961 catccagccc tgcgcccact gtccggcccg ccgccgcacc tcggggtggt tcccccccgc
     1021 gtagatcggc atcgggctct gcacgggctt cgggaagcac tccacctcct cgaagtggac
     1081 cgcttgcccg cggaacgtcg cccgccgctc ggtgaagagc agccgcagcg cctgcatgcc
     1141 ctcgtccacg atggtgcccc gacgcgcccc gcgcgcgtcc gggaagagcg cctcgtactc
     1201 ctctcggtac gccccggtgc cgacccccag gatcacgcgc ccacccgaca gctggtccag
     1261 cgtggcgacc tgcttggcga ggaccggcat gttgcgcatc ggcagcacga tgatgccggt
     1321 ggcgaacctc aggcgcgtcg tccgcgaggc gcagtaggcg aaggtcatca gcggctcgaa
     1381 gaagttcggc gggtccggga actccttctg cacgtagcgc tgggtcgtca tgtggtcgtt
     1441 gccccacacc gagtcgaacc cgagccgctc gcagagcagc gcggtgggca ggatgtcgga
     1501 cggcttcgcg aagggaaccg ggtacatcat cccctcgatg cacgtcggga tgtggacgcc
     1561 gaatcgcatg ccccgggcct cagtcgagct tcggggggga ccaggcccga atgagctgca
     1621 ccttgccgtc cggcccccac gtcccgatga acacggtggt gtcgatctcc ccgttggggg
     1681 tgatcgtgta ggtgccggcc gccccgctga tcggaccggt cttgaacatc tccggaatga
     1741 ccttggctcc gtcggtgctc ttggcccgct ccatcgcccc ggccacgatc atgaccgcgt
     1801 cgtaggcgaa catcgaccac tcgtcgccct cgagcgcggt gtacttggcg tggaagcctt
     1861 tctcgaagtc gaccgcttcc ttgttcttga agtgcccggg ccagaacgca ggaacgacga
     1921 tcatcccatc ggaggcgctg cccgcgatgc cccggaaggc cttgctcgaa tgtcccccgc
     1981 ccccgaccag gggcaccttg agaccaagct cgccgtattg cttgacgagc agggcggtgt
     2041 cggcgagcga gccggtgacc acgatggcgt cgggcccgag cccggccagc ttcgtgagga
     2101 gcggccggaa ctccttggcg tcgagccggt accactcctt gcccaccacc tcggcgccgc
     2161 cgaacttctt gaggtagcgg tcgatcgcgt ccacgttgcc cttgccgtac tcgtcgtcga
     2221 gggccatcac cgcgagcttg cggtacttct tctccttgac cgcgtagtcg agcgcggcgg
     2281 aggtgaggtg gtccgagttg ggcttcagat tgatcacgtg cttgtagccg gaccgggtga
     2341 tcttgatcgc gttcgtgtag gtgatctggg gcacgttggc ccgggcgagg atgtcgatga
     2401 tcgccagcgt gccgctgctg gtgaagtcgc cgaagtaggc gtgaaccttc tcctgcagcg
     2461 tgtagcggcg cgcgatgttg atggcctcgt tggggtcgag ccgggtgtcc ccgtacgaca
     2521 cctcgacctt ccgcccgagg atcccgccct tggcgttcag ctccgcgacc gcgtactcgg
     2581 tcccgagctt ggcgcgctcg gcgatgaagg cgatcggcga cgtgaggggg gcggagaagc
     2641 cgaacttcac cggatcggcg gcgaaacccg ggatcggcgc gaggccgatc gccgcaagcg
     2701 cgagcgaggc gagcagagtg aagagcttca tgcgcgaaag tctcccattc ttaccacatt
     2761 cgaggatcat ccgaggtacg cggccacgac ccgggcgtcc tgccggaggg ccgccgccgg
     2821 gccctccgcc acgatcgagc cctgctccag cacgtacgcc cgggacgcca gctcgagcgc
     2881 gatgtgcgcg ttctgctcca ccagcaacac catggtgccg cgtcgatgca ggtcctggat
     2941 ggtccggaag atcgactcca ccatggtcgg agccagcccg gtcgacggct cgtcgaggat
     3001 gagcagcgtc gggttcgaca tcagggcccg cgcgatggcc agcatctgct gctcgccgcc
     3061 ggacagggtg ccgcccatct gccgcaggcg ctcggcgagc gcggggaacg cgctcagcac
     3121 gcgctccacc cgaccctcga tctcgccggc cgcggcacgg tacgcgccca ggcgcaggtt
     3181 ctcgcgcacg gtgag
//
LOCUS       DATMCV010000619         3758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_695912, whole genome shotgun sequence.
ACCESSION   DATMCV010000619 DATMCV010000000
VERSION     DATMCV010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695912"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1869)
                     /gene="kdpA"
                     /locus_tag="VNU03_14295"
     CDS             complement(64..1869)
                     /gene="kdpA"
                     /locus_tag="VNU03_14295"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWP75688.1"
                     /translation="MTTNGIVQLVFYVVVLLVLAQPLGAYMARVYEGRPLALDRAFGW
                     LERLIYRAGGIRPTEEMGWKTYALTMLTFNFLGLLAVYFLQRAQGILPLNPQGMAGVS
                     PDSSFNTAVSFATNTNWQGYAGESTMSYLSQMLALTVQNFVSAASGMATMVALIRGFA
                     RRSVETIGNFWVDLTRTTLYILLPLAVILALALVSQGVVQTFGAYPKATVLQTSEYDE
                     PVTDKDGKPVLDAKGQATTKKSKLTEQVIAVGPAASQIAIKQLGTNGGGFFNANSAHP
                     LENPTPLANFLEMLSILLIAAALCHTFGVMVGDTRQGWTVLAAMTVIFVVLLGTCYLA
                     EQNGAVFVKQGVDHTASALQSGGNMEGKETRFGIASSAIWATATTSASNGSVNSMHDS
                     YTPMGGLVPMWLMQLGEVVYGGVGSGLYGMLMFAIVAVFVAGLMVGRTPEYLGKKIEA
                     YEMKMASLVILITPTLVLVGTAVGVVTVAGKAGIANPGIHGFSEILYAFSSASNNNGS
                     AFAGLSANTPFYNTALGLCMLFGRYWLAIPVLAIAGSLARKKTVPTGPGTLPTATPLF
                     VGLLVGVVILVGALTFVPALALGPIVEHLTLIAAK"
     gene            complement(1866..1958)
                     /gene="kdpF"
                     /locus_tag="VNU03_14300"
     CDS             complement(1866..1958)
                     /gene="kdpF"
                     /locus_tag="VNU03_14300"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HWP75689.1"
                     /translation="MPLLYVVGAVVSLGLLVYLVLALLKPELFQ"
     gene            complement(2293..3126)
                     /locus_tag="VNU03_14305"
     CDS             complement(2293..3126)
                     /locus_tag="VNU03_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWP75690.1"
                     /translation="MLRSALVALDGSACGESAADLAIDWAGRYGAHLLGLGILDAPTI
                     QRAEPVPLGAGAYKKARDQARLADAHRRVADLLTAFRARGQAAGVAVEVLEDIGDPAV
                     RILREAQRCDVVILGRETHFHFETQDEPDGTLAQVLRGSPRPVVVVPRSVPQGHGVLL
                     AYGGGREAARTLQTFQLLGLAAGEAIEVVTIHRDGAEAEALVNLAGEYLTAQGAPHRL
                     HPIVSATPPADVLLEQVRRARPRLLVMGAQGHHPLRDLFGASVTRAVMRACPVPAFIG
                     A"
     gene            3261..3719
                     /locus_tag="VNU03_14310"
     CDS             3261..3719
                     /locus_tag="VNU03_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWP75691.1"
                     /translation="MLPISEVMRHEIASVSPDAPVRRVVEVMVETGVEEVLVVDGRGR
                     VIGSVGDDQLVVSLHASQSRSWWGRLRGDDDGGPEQRLSSLAAADVMLTRVFPVDAAT
                     SATSAARLFVEYGVNVMPVVDHGVLVGVVFRGDLIKRLLLPYAFARSART"
BASE COUNT          626 a         1257 c         1308 g          567 t
ORIGIN      
        1 gacgatcgcg gtctcgaaga gcgagcgtgc ccgtaccgcc gtggtggcca tgtcagagac
       61 tccctacttc gccgcgatca aggtgagatg ctcgacgatc ggcccgaggg ccagcgccgg
      121 cacgaacgtc agcgcgccga ccaggatcac cactcccacc agaagtccca cgaacagcgg
      181 ggtcgcggtg ggcagcgtgc cggggcccgt cggcaccgtc ttcttccggg ccagcgatcc
      241 cgcgatggcc aggaccggga tcgccagcca gtagcgaccg aagagcatgc agaggccgag
      301 cgcggtgttg tagaacgggg tgttcgcgga gagcccggcg aacgcgctgc cgttgttgtt
      361 ggacgccgag gagaaggcgt agaggatctc gctgaagccg tggatgcccg gattggcgat
      421 gccggccttc cccgccaccg tgacgacccc gaccgcggtc ccgaccagga cgagcgtcgg
      481 ggtgatgagg atgaccaggg aggccatctt catctcgtag gcctcgatct tcttgcccag
      541 gtactcggga gtgcggccga ccatcaggcc ggccacgaag accgcgacga tcgcgaacat
      601 gagcatgccg tagaggccgg agcccacccc gccgtacacg acctcgccga gctgcatcag
      661 ccacatgggc accagccctc ccatcggcgt gtaggagtcg tgcatcgagt tgaccgagcc
      721 gttcgaggcc gaggtggtgg cggtggccca gatcgccgag ctcgcgatgc cgaagcgagt
      781 ctccttgccc tccatgttgc cgcccgactg gagcgcgctc gcggtgtgat ccaccccctg
      841 cttcacgaag accgcgccgt tctgctcggc caggtagcac gtcccgagca gcaccacgaa
      901 gatcacggtc atggcggcca gcaccgtcca cccctggcgc gtatcgccga ccatcacccc
      961 gaacgtgtgg cagagcgcgg ccgcgatcag aagaatcgac agcatctcca ggaagttcgc
     1021 gagcggagtc gggttctcga gcggatgagc cgagttggcg ttgaagaacc caccgccgtt
     1081 ggtgccgagc tgcttgatgg cgatctggga ggcggccggg cccaccgcga tcacctgctc
     1141 ggtgagcttg gacttcttgg tcgtggcctg ccccttcgcg tcgaggaccg gcttgccgtc
     1201 cttgtcggtc accggctcgt cgtactcgct cgtctggagc accgtcgcct tgggataggc
     1261 gccgaaggtc tggaccacgc cctgggacac gagggccagc gcgaggatca ccgcgagcgg
     1321 cagcaggatg tagagcgtgg tccgggtgag gtcgacccag aagttgccga tggtctcgac
     1381 cgagcggcgc gcgaagccgc ggatcagcgc gaccatggtg gccatcccgg acgccgcgga
     1441 gacgaagttc tgcaccgtca gggcgagcat ctggctcagg tagctcatgg tggactcgcc
     1501 ggcgtaaccc tgccagttcg tgttcgtggc gaagctcacc gcggtgttga acgacgagtc
     1561 gggcgagacg ccggccatgc cctgcgggtt cagcggcagg atgccctgcg cccgctggag
     1621 gaaatagacc gcgagcagac ccaggaagtt gaacgtgagc atcgtgagcg cgtaggtctt
     1681 ccagcccatc tcctcggtgg ggcgaatccc gcccgcccgg tagatgaggc gctccagcca
     1741 accgaaggcg cggtccagcg cgagcgggcg cccctcgtac acccgcgcca tgtacgcccc
     1801 gaggggctgg gcgaggacga gcagcaccac cacgtagaag acgagctgga cgatcccgtt
     1861 cgtggtcatt gaaagagctc cggcttcagc agagcgagca ccaggtagac cagcaggccc
     1921 agggacacca ccgcgccgac gacgtagagc aagggcatgg atgcctccta gagacgctca
     1981 cagagccgga cgaggagcca cgacagccag aagaacgcac cgtcaccccg aggtacagaa
     2041 cgtctgccat cgccaggtct ccttgtccgc ctctttcccg accttccaac ctgaaatcct
     2101 agccacgccg ccgtaagggc ggccttaaga accggccgcc gggcatcaag aaggcgtcaa
     2161 ggcgccccca acgccacgaa ttcccagggc ggcggcccgg tccctccaat gcctcatcga
     2221 tatggatgcg cctgccgagc ctccggttga ggcactctcg agaaaatctc gaccgcctgg
     2281 cgccgtctcc gctcaggcgc cgatgaaggc aggaacgggg cacgcccgca tgaccgcgcg
     2341 cgtgaccgag gccccgaaga gatcgcggag gggatggtgg ccctgggcgc ccatcaccag
     2401 caggcggggg cgggcgcggc gaacctgctc caggaggacg tcggcgggcg gcgtggccga
     2461 gactatggga tgcagccgat gcggggcccc ctgtgcggtc aggtactccc ccgccaggtt
     2521 gaccagcgcc tcggcctcgg cgccgtcgcg gtggatcgtc acgacctcga tggcctcgcc
     2581 ggcggcgagc ccgaggagct ggaaggtctg gagcgtgcgt gcggcttctc gaccgcctcc
     2641 ataggccagg aggacgccgt gtccttgcgg gaccgaccgg ggaacgacca ccaccggcct
     2701 ggggctaccg cgtaagacct gggcgagcgt cccgtcgggc tcgtcctggg tctcgaagtg
     2761 gaagtgcgtc tctcgcccga ggatgaccac gtcgcagcgc tgggcctcgc gcaggatgcg
     2821 gacggctggg tcgccgatgt cctcgagcac ctcgaccgcc acccccgccg cctgaccacg
     2881 cgcgcgaaag gcggtgagga gatccgccac ccgccgatgc gcgtccgcca gcctggcctg
     2941 atcgcgggcc ttcttgtagg cgccggcccc gaggggaacc ggctccgctc gctggatggt
     3001 gggcgcgtcg aggataccga gaccgagcag gtgggcgccg tatcggccag cccagtcgat
     3061 ggcgagatcg gccgcactct cgccgcaagc cgagccgtcg agcgcaacga gcgccgatcg
     3121 aagcatgggc gggcctccct gcgccaaagt tagtccggta ctcggtcaag gcgtcgtcaa
     3181 gattcgtctt tacagttcgt tgatccgtcg tggctccgct ttgacgctgt cctgatgggc
     3241 tccgcccctc tactggagcc atgcttccca tcagcgaggt gatgcggcac gagatcgcgt
     3301 cggtctcccc ggatgcgccg gttcgccggg tcgtcgaggt catggtggag acgggggtcg
     3361 aggaagtgct cgtggtcgac gggcggggcc gcgtgatcgg gtccgttggc gatgaccagc
     3421 tggtcgtaag cctgcacgcg agccagagcc gctcgtggtg gggtcggctc cgtggggacg
     3481 acgacggagg gcccgagcag cgtctgtcga gcctggccgc ggcggacgtc atgctgacgc
     3541 gcgtgttccc ggtagatgcc gccacgtccg cgacgtcggc ggcccggctc ttcgtcgagt
     3601 acggggtgaa cgtcatgccg gtcgtcgatc acggggtcct cgtcggcgtg gtcttccgcg
     3661 gcgatctgat caagcgcctg ctccttccct acgcgttcgc ccgctcggcc agaacgtagc
     3721 gaggacccgg gcgatgagtt gaacccggcg cgtgttcc
//
LOCUS       DATMCV010000620         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_231971, whole genome shotgun sequence.
ACCESSION   DATMCV010000620 DATMCV010000000
VERSION     DATMCV010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231971"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VNU03_14315"
     CDS             <1..195
                     /locus_tag="VNU03_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75692.1"
                     /translation="GLDLLGHLAATGSRIPVIILTAHSDDEIRRRTLQAGAVAHLGKP
                     FHGEALLDAVRNALARAGRP"
     gene            222..680
                     /locus_tag="VNU03_14320"
     CDS             222..680
                     /locus_tag="VNU03_14320"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWP75693.1"
                     /translation="MTTGQLASAGPSALEVTTPHETHVPGWPGPAPREIGTLPLSRLR
                     RVTDYIREHLDQDLTLSQLGGVVYMSPFHFARLFQRSTGLPPHRFVVRARIARAAALL
                     AEPEPPIAQISRVVGFRTPSHFSTVFRRLMGVTPHEYRAKRLEGARDERN"
     gene            667..1350
                     /locus_tag="VNU03_14325"
     CDS             667..1350
                     /locus_tag="VNU03_14325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP75694.1"
                     /translation="MNATSRVMERPLREGTMDAPPLPVRRGTAADRDAALVERLRRHE
                     EGAAEALVAEYGNRAYRLAMRITNNSSDAEEVVQDALWAASRKIETFRGTAAFGSWVY
                     RITANAAYQKRRARRHDRNETSWEDLAPTFDETGQPVQGGSDWSPRLHDPALQSELHA
                     VLRSAIDELPGEHRATFLLHDVEGLSNPEIAETLQIKTATVKSRVHRARLFLRGRLAI
                     YLGGTPDLT"
     gene            complement(1372..2112)
                     /locus_tag="VNU03_14330"
     CDS             complement(1372..2112)
                     /locus_tag="VNU03_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP75695.1"
                     /translation="MSGSSGKVAMVTGASGGIGRAVALRLARDGFAVVATYAGNPARA
                     QELVTEIEAADGRAIAVPADVASAPEVDRLFRDTLEAFGRIDVVVNSAGIMPLGQIAD
                     GKLEDFDRVIATNLRGAFLVLGHAARHVGRGGRIVALSSSVIAKSLPGYGAYIASKAG
                     VEGLVRVLANELRGHDVTVNAVAPGPVATELFLRGKTEAQIEQFAKLAPLERVGRPED
                     VAEVISFLAGPGGAWVNAQVLRANGGFA"
     gene            2245..2883
                     /locus_tag="VNU03_14335"
     CDS             2245..2883
                     /locus_tag="VNU03_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWP75696.1"
                     /translation="MSAAGDPVIFVVDDDRSVRESLRRLLTSVGLTVEVFPSAQAFLG
                     TPRPDSPACLVLDVRLPGLSGLDLQRELSRTDVSLPIVFLTGHGDIPMSVRAIKAGAV
                     EFLTKPFREKDLLAAIRQAIERDRAGRQERQEVAELRRRYETLTPRQREVMTRIVAGS
                     LNKQIAADFGTTEATVKEQRGQVMAKMRAGSLAELVRIAGRLEVTAAGSRGA"
     gene            2941..>3142
                     /locus_tag="VNU03_14340"
     CDS             2941..>3142
                     /locus_tag="VNU03_14340"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HWP75697.1"
                     /translation="MASVAVAAALTTGPGGERLFPEAPERFVHGVTLLYSAAVLSAWL
                     GGMGPGLLAALLAAVVIDWFITP"
BASE COUNT          432 a         1180 c         1106 g          424 t
ORIGIN      
        1 ggcctcgacc tgctgggcca cctggccgcc accggctctc gaatcccggt gatcattctc
       61 accgcccaca gcgacgacga gatccgccgg cgaactctgc aggcgggcgc ggtcgcgcat
      121 ctgggtaaac cattccacgg cgaggccctg ctggacgccg tgcgcaacgc cctggcccga
      181 gccggccgtc catgaccggc cgggcgcaag aggagacgac gatgacgaca ggacagctcg
      241 cgagcgcggg accgagcgcg ctcgaggtaa cgactccaca cgaaacccat gtcccgggat
      301 ggccgggacc agccccgcgc gagatcggca cgctccctct ctcgaggctg cggcgggtca
      361 ccgactacat ccgtgagcac ctcgaccagg acctgaccct ctcgcagctc gggggggtgg
      421 tgtacatgag cccgttccac ttcgcccgcc tgttccagcg cagcaccggg ctcccgccgc
      481 accggttcgt ggtgcgtgcg cgcatcgccc gcgccgccgc cctgctcgcg gagccggagc
      541 cgcccatcgc ccagatctcc cgcgtggtgg gattccggac cccaagtcac ttctccaccg
      601 tgttccgccg gctcatgggc gtgaccccgc acgagtaccg agccaagcgg ctcgagggag
      661 cccgcgatga acgcaactag ccgcgtgatg gagcgtccgc tccgcgaggg aacgatggac
      721 gcgccgccgc tgccggtgcg gcgcggcacg gcggccgacc gggacgcggc gctggtggag
      781 cggctgcgcc ggcacgagga gggcgcggcg gaggcgctgg tggccgagta cggcaatcgc
      841 gcctatcgcc tggccatgcg catcaccaac aacagctcgg acgccgagga ggtcgtgcag
      901 gacgcgctct gggccgcctc gcgcaagatc gagactttcc gcgggacagc tgcctttggc
      961 tcgtgggtct accgcatcac cgccaacgcc gcctaccaga agcggcgcgc gcggcgccac
     1021 gaccggaacg agacgtcgtg ggaggatctc gccccgacct tcgacgagac cggccagccg
     1081 gtccagggcg ggagcgattg gtcgccgcgg ctccacgacc cggcgctcca gtccgagctg
     1141 cacgcggtgc tccgctccgc catcgacgag ctgccgggcg agcatcgcgc gaccttcctg
     1201 ctacacgacg tcgaggggct ctcgaacccg gagatcgcgg agacgctgca gatcaagacc
     1261 gccaccgtca agtcgcgggt gcaccgggcc cggctcttcc tgcgggggcg tctggcgatc
     1321 tacctgggcg ggacgcccga tctgacgtga gggcccgagt cgacgtgacg atcaggcgaa
     1381 gccgccgttg gcgcggagca cctgcgcgtt gacccacgcg cccccgggcc cggccaggaa
     1441 cgagatcacc tccgcgacgt cctcgggccg tccgacgcgc tcgagcgggg cgagcttggc
     1501 gaactgctcg atctgcgcct cggtcttgcc ccggaggaac agctcggtcg ccaccgggcc
     1561 cggcgccacc gcgttgaccg tcacgtcgtg gccgcgcagc tcgttggcga gcacgcggac
     1621 caggccctcc acgcccgcct tcgaggcgat gtaggcgccg tagccgggca acgacttggc
     1681 gatgacgctg ctcgagagcg cgacgatgcg gccgccgcgg ccgacgtgcc gggccgcgtg
     1741 gcccagcacg aggaaggcgc cgcgcagatt ggtggcgatc acccggtcga agtcctccag
     1801 cttgccgtcc gcgatctggc caaggggcat gatgccggcg ctgttcacca ccacgtcgat
     1861 ccgcccgaag gcctcgagcg tgtcccggaa gaggcggtcc acctcgggag cgctcgccac
     1921 gtccgccggg accgcgatcg cgcggccgtc cgcggcctcg atctcggtca ccagctcctg
     1981 ggctcgcgcc gggttgccgg cgtaggtcgc caccaccgcg aagccatcgc gggccagtcg
     2041 cagggccacc gcgcggccga tgccgcccga ggctccggtc accatcgcca ccttgcctga
     2101 cgagccgctc atcgcgtccc ctctccgcct cgctctttcg agggcgccgc gcgcgaggcg
     2161 gcggctcgcc gcgatcgttt cggcaagaac gcgacacgtc gcccgcccgg ccacggtggc
     2221 cctgcgtggt atcgtcaacg tgccatgagc gcggcgggtg atccggtcat cttcgtggtc
     2281 gacgacgacc ggtccgtgcg cgagtcgctg cggcggctcc tcacctcggt cgggttgacc
     2341 gtcgaggtgt tcccgagcgc gcaggcgttc ctgggcacgc cccggccgga ctcgcccgcc
     2401 tgcctggtgc tcgacgtccg cctcccgggg ctgagcggcc tcgacctcca gcgcgagctg
     2461 tcccgcaccg acgtctcgct gccgatcgtc ttcctgaccg gccacggcga catcccgatg
     2521 tcggtccggg ccatcaaggc gggggcggtc gagttcctca ccaagccgtt ccgcgagaag
     2581 gacctgctcg ccgcgattcg ccaggcgatc gagcgcgacc gcgcgggccg gcaggagcgg
     2641 caggaggtgg ccgagctgcg gcgccgctac gagacgctga ccccgcggca gcgcgaggtc
     2701 atgacccgca tcgtcgccgg ctcgctcaac aagcagatcg cggccgattt cggcacgacg
     2761 gaggccaccg tcaaggagca gcgcggacag gtgatggcca agatgcgagc gggctcgctg
     2821 gcggagctgg tccggatcgc gggccgcctg gaggtgaccg cggccggctc gcggggcgcg
     2881 tgaatggcga cccccgcccg cccctctcgg ctcccggtct cgctccgatg ggcgctggcg
     2941 gtggccagcg tcgcggtcgc ggccgcgctc accaccgggc ccgggggaga gcggctcttc
     3001 ccggaggcgc cggagcgctt cgtccacggc gtcaccctcc tctactccgc cgcggtgctg
     3061 agcgcgtggc tcggcgggat gggacccggg ctgctcgcgg ccctcctcgc cgcggtggtc
     3121 atcgactggt tcatcactcc cc
//
LOCUS       DATMCV010000621         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_30833, whole genome shotgun sequence.
ACCESSION   DATMCV010000621 DATMCV010000000
VERSION     DATMCV010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30833"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VNU03_14345"
     CDS             <1..508
                     /locus_tag="VNU03_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75698.1"
                     /translation="PLANGENDFTESLQLPGEAPVRDPAPGALAIQAYLARAEWLSQS
                     ANPVAFAPYLRQAPLPGVPARPVLLQWAVGDQTVPNPTTRALLEAGLLRPVSSLYRHD
                     VLGPGLPDRFRDPHAFLTWTAFPEVYAIGRAAQEQVARFFLSGGRQIEAVIPQFETAG
                     RLPAAAGR"
     gene            510..1331
                     /locus_tag="VNU03_14350"
     CDS             510..1331
                     /locus_tag="VNU03_14350"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP75699.1"
                     /translation="MKAVLLGTGSPPPNPQRRGPSTLLSLGAERFLVDAGSGVGVQLV
                     QAGVRPYEWPRLFITHHHSDHVIDLGHLLITRWIVGQNAPLEIWGPAGTRRQVDKLLD
                     YLHWDIEIRRSHMVDRKPPAVTVTEIEEGRVMEAGGVTVSAFLVEHDPVRPAFGYHFE
                     GGGRRVVISGDTRPCENLIRWSQGVDVLIHECCEMAKTSWYPGCGWPSLEEKVRDLAS
                     YHTQPDDLGRVAERARAKKLAVTHLMPGSEPRALEHAARRHFAGPLVVGSDLLEV"
     gene            complement(1328..1804)
                     /locus_tag="VNU03_14355"
     CDS             complement(1328..1804)
                     /locus_tag="VNU03_14355"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWP75700.1"
                     /translation="MSRSLGPALPADLLAQLSQADLESQLGRAMPFVTQDADGTPHPM
                     LCSYLEWLAVDATTLRLAIAAGSRSARNLEARRVGTLLVIEPARTVYVKCRARGAARR
                     VGDLARFTLAVEDVLDDRPAEAEGDARIASGITYRPRPSLDAPWARAVLDALRAEG"
     gene            complement(1808..>2208)
                     /locus_tag="VNU03_14360"
     CDS             complement(1808..>2208)
                     /locus_tag="VNU03_14360"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75701.1"
                     /translation="GTDEVLATSDWVLLLLPVTPETRGFMSAARLKRMKPSAYLLNFG
                     RGELVVDGDLIEAVKAGTIKGAVLDVFTTEPLPAEHPFWGTEGILVLPHIGGLHPGRD
                     HIVAKLFVDNVERFLAGQPLRETVDLARGY"
BASE COUNT          285 a          823 c          803 g          297 t
ORIGIN      
        1 gcccctggcc aacggggaga acgacttcac cgagtccctg cagctgcccg gcgaggcccc
       61 ggtgcgcgac ccggcgccgg gcgcgctcgc catccaggcc tacctcgccc gcgcggagtg
      121 gctgtcccag agcgccaacc cggtcgcctt cgctccctac ctgcggcagg caccgctgcc
      181 cggcgtgccg gcgcgccccg tcctgctcca gtgggcggtg ggcgatcaga ccgtccccaa
      241 cccgaccacg cgggcgctgc tcgaggcggg cttgctccgg ccggtctctt ccctctatcg
      301 tcacgacgtg cttgggcccg gtctcccgga ccgcttccgc gacccccacg ccttcctgac
      361 ctggaccgcg ttccccgagg tgtacgccat cgggcgcgcg gcgcaggagc aggtggcgcg
      421 cttttttctc tccggcggcc ggcagatcga ggcggtgatc ccgcagttcg agaccgcggg
      481 ccgcctgccc gcggcggcgg ggcgctgacg tgaaggcggt cctgctcggc accggctcgc
      541 caccgcccaa cccccagcgg cgcggcccgt cgacgctgct ctcgctcggc gcggagcgct
      601 tcttggtgga cgcgggctcg ggggtgggcg tccagctcgt gcaggccggc gtgcgtccct
      661 acgagtggcc gcggctgttc atcacccacc accactccga ccacgtgatc gacctcggcc
      721 acctgctcat cacccggtgg atcgtggggc agaacgcccc gctcgagatc tggggcccgg
      781 ccggcacgcg gcggcaggtg gacaagctgc tcgactacct ccactgggac atcgagatcc
      841 gccgctcgca catggtcgac cgcaaaccgc ccgcggttac ggtcaccgag atcgaggagg
      901 ggcgcgtcat ggaggcgggc ggcgtcacgg tcagcgcgtt cctggtcgag cacgacccgg
      961 tgcgtcccgc cttcggctac cacttcgagg gcggcgggcg ccgcgtcgtg atctcgggcg
     1021 acacgcggcc ctgcgagaac ctgatccgct ggagccaggg cgtcgacgtg ctgatccacg
     1081 agtgctgcga gatggccaag acctcgtggt atccgggctg cgggtggccg agcctcgagg
     1141 agaaggtccg cgacctggcc tcctaccaca cccagcccga cgacctgggg cgcgtcgccg
     1201 agcgcgcgcg ggcgaagaag ctcgcggtca cccacctgat gcccggctcc gagccgcggg
     1261 cgctcgagca cgcggcgcgg cggcacttcg cggggccgct ggtggtgggc agcgacctcc
     1321 tcgaggtcta gccctccgcg cgcagcgcgt ccagcaccgc gcgcgcccac ggcgcgtcga
     1381 gcgacggccg cggccggtag gtgatcccgc tcgcgatccg cgcgtcgccc tcggcctcgg
     1441 cgggtcggtc gtcgagcacg tcctccaccg cgagcgtgaa gcgcgcgagg tcgcccacgc
     1501 gacgcgcggc gccccgcgcg cggcacttca cgtagaccgt acgggccggc tcgatcacga
     1561 ggagggtgcc gacccggcgg gcctccaggt tgcgcgcgct ccggctcccg gccgcgatgg
     1621 cgagacggag cgtcgtcgcg tcgaccgcga gccactcgag ataggagcag agcatcgggt
     1681 gcggcgtgcc gtcggcgtcc tgggtgacga agggcatcgc gcggccgagc tgggactcga
     1741 gatcggcctg ggagagctgc gcgagcaggt ccgcggggag cgcgggcccg agcgagcggc
     1801 tcaccgctca gtagccgcgg gcgagatcca ccgtctcccg gaggggctgg cccgcgagga
     1861 agcgctcgac gttgtccacg aagagcttgg ccacgatgtg atcgcgcccc gggtggagcc
     1921 cgccgatgtg gggcagcacc aggatgccct cggtgcccca gaacgggtgc tcggcgggca
     1981 ggggctcggt ggtgaagacg tccagcaccg cgcccttgat ggtgcccgcc ttcaccgcct
     2041 cgatgaggtc tccgtccacg accagctcgc cccgcccgaa gttgagcagg taggcggagg
     2101 gcttcatgcg cttgagccgg gccgcgctca tgaagccgcg cgtctcgggc gtcaccggca
     2161 ggagcagcag cacccagtcg gacgtggcca gcacctcgtc ggtcccct
//
LOCUS       DATMCV010000622         3343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_500549, whole genome shotgun sequence.
ACCESSION   DATMCV010000622 DATMCV010000000
VERSION     DATMCV010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500549"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VNU03_14365"
     CDS             <1..498
                     /locus_tag="VNU03_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWP75702.1"
                     /translation="NNARTTDPVLGVQVTDGKQRVQGAELEIVGRVLPGWNVWLSYAY
                     LDSRVLDSLDIQTGVPVQGKRLPNVPEHSASLWTTYDITPQWQVGGGAVFVDKRYANT
                     DNLNVVPSYVRGDLTAAYRPIKPLELRFNVINVSDERYFDAIHPSHVIPGNARTFLLT
                     GTWRF"
     gene            506..1186
                     /locus_tag="VNU03_14370"
     CDS             506..1186
                     /locus_tag="VNU03_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HWP75703.1"
                     /translation="MLLHIPKILTDSQVGHCRALLEEAKWVDGRETSGHLAARVKYNR
                     QVDEGTPEAREMGNIIVTALERTPLFMAAALPLRVFPPLFNRYEPGMAFGAHVDNAIR
                     ELTSSPQRVRTDLSATLFLSRAEEYDGGELVVDDTYGAHAVKLPAGDMILYPASSLHR
                     VNPVTRGVRLASIFWVQSMVADDAERSLLFDLDMAISQVTGTSPDSPAVVALTGCYHN
                     LLRRWSQV"
     gene            1197..1943
                     /locus_tag="VNU03_14375"
     CDS             1197..1943
                     /locus_tag="VNU03_14375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWP75704.1"
                     /translation="MIRQGALLSWPLWTSVGIHVVGLATAGSVVALARHDVERVLVPV
                     EVVRVEPPPPAPPEKPKMPRQITQPTRLRATPTPQTLMQDPTPRTERPPDPAAASPER
                     RYMASNEVPGPALPIPGPAGDGGLLLPSAEIPGAPSGRSSIGAGVARGGGEGVTSFAR
                     PLGGYQTTPNYPEAARREGVEGTVTLRFEVLASGKVGTVQVQRSAGRPDMDRAAVEAI
                     KTWLFEPARRGKEAVAVWVTLPVRFELNVR"
     gene            1991..2791
                     /locus_tag="VNU03_14380"
     CDS             1991..2791
                     /locus_tag="VNU03_14380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP75705.1"
                     /translation="MGLAFCAIATVGTASAQGIWDDPAFSLYRQAVEAMDKKDYARAT
                     DLSGQAIAQQPTHVLAHYLRGQAAMYQSRWDDAAAAFGKVVELYPGSFAGHRDLGGAY
                     EQLNRVDDAARQYEAAIALRDQDDLRVRLAFMLIKANQQPRALPHLQTLADRDSKAPE
                     VWSALARVAYDKSDLPAAEKNFTRAAALRDDGRTWFNLGVVRTRLNDLPGALQAFEKA
                     AQHSETKEPAQKEVAKVREAMKQPTTGTAPDRAMPGSTPGQPVPTRRY"
     gene            2799..>3343
                     /locus_tag="VNU03_14385"
     CDS             2799..>3343
                     /locus_tag="VNU03_14385"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWP75706.1"
                     /translation="MSIRPVVFWLHLLVGVTAGTVIAFMSVTGVLLAFEPQITDWLER
                     DRRIVVPPPGASRLPVETLVARAREARPDVRPSIVTLRADPTAALVVSFGRDGGALFI
                     DPYRGTVLGGFSRVHDMLHEVVEWHRWLGSREIARPITGAANLGFLGLVSLGAFLWWP
                     RCWTSAALRQVTRFDASLRGRA"
BASE COUNT          482 a         1251 c         1149 g          461 t
ORIGIN      
        1 aacaacgcgc gcaccaccga tccggtgctg ggcgtccagg tgaccgacgg caagcagcgc
       61 gtccagggcg ccgagctcga gatcgtggga cgggtgctgc ccggctggaa cgtctggctg
      121 agctacgcct atctcgactc ccgggtcctc gactcgctgg acatccagac cggggtgccg
      181 gtgcagggca agcgcctccc caacgtgccg gagcactcgg cgagcctgtg gaccacctac
      241 gacatcaccc cccagtggca ggtgggcggg ggcgcggtct tcgtcgacaa gcgctacgcc
      301 aacaccgaca acctcaacgt ggtgccgtcg tacgtgcgcg gcgacctgac cgcggcgtac
      361 cgcccgatca agccgctcga gctgcgcttc aacgtgatca acgtctccga cgagcgatac
      421 ttcgacgcga tccacccgag ccacgtgatc ccgggcaacg cccgcacgtt cctgctcacg
      481 ggcacctgga ggttctagcc gggcgatgct cctgcacatt cccaagatct tgaccgacag
      541 ccaggtcggg cactgccggg ccctcctgga ggaggccaag tgggtggatg gtcgcgagac
      601 ctccggtcac ctggccgccc gggtcaagta caaccggcag gtggacgagg gaacccccga
      661 ggcgcgcgag atgggcaaca tcatcgtcac cgcgctcgag cgcacgccgc tgttcatggc
      721 cgccgcgctg cccctgcggg tgttcccacc gctgttcaac cgctacgagc ccgggatggc
      781 gttcggggcg cacgtcgaca acgccatccg cgagctgacg agctcgcccc agcgggtccg
      841 caccgatctg tcggccacgc tgttcctgag ccgcgccgag gagtacgacg gcggcgagct
      901 ggtggtggac gacacctacg gcgcgcacgc ggtcaagctg cccgccggcg acatgatcct
      961 gtacccggcg tcgagcctgc accgggtcaa cccggtcacg cgcggcgtgc gcctggcgtc
     1021 gatcttctgg gtgcagagca tggtggccga cgacgcggag cgctcgctgc tgttcgacct
     1081 cgacatggcg atcagccagg tcaccgggac atcgccggac agccccgcgg tggtcgcgct
     1141 caccggctgc taccacaacc tgctgcggcg ctggtctcag gtctgaggag acacccgtga
     1201 tacggcaagg cgcgctgctc agctggccgc tctggacctc ggtcggcatt cacgtggtcg
     1261 gcctcgcgac ggcgggctcg gtagtggccc tcgcgcgcca cgacgtggag cgcgtgctgg
     1321 tcccggtcga ggtcgtccgc gtggagccgc caccgcccgc gccgcccgag aagcccaaga
     1381 tgccgcggca gatcacgcag ccgacccggc tgcgcgccac gcccaccccg cagaccctca
     1441 tgcaggaccc gaccccgcgc accgagcggc cgccggatcc agccgccgcc tcgcccgagc
     1501 gacgctacat ggcttccaac gaggtccccg gccccgcgct gccgatcccg ggaccggccg
     1561 gggacggcgg cctcttgctc ccctcggccg agatcccggg cgcgccgagc ggccggtcga
     1621 gcatcggcgc cggcgtggcc cgcggcggcg gcgagggcgt gacgtcgttc gcccgcccgc
     1681 tgggcggcta tcagaccact cccaactacc cggaggccgc gcggcgcgaa ggcgtcgagg
     1741 gcacggtcac cctccggttc gaggtgctcg ccagcggcaa ggtgggcacc gtgcaggtgc
     1801 agcgctccgc ggggcgcccg gacatggacc gcgccgcggt cgaggcgatc aagacctggc
     1861 tcttcgagcc agcccgtcga ggaaaggagg cagtcgccgt atgggtcacc ctaccggtac
     1921 gcttcgagct gaacgtccgc tgagccgcac ccgatccccg cgcttcccga ggggtcacca
     1981 ctggaccgcg gtgggcctgg ccttctgcgc catcgccacc gtgggcaccg cgtccgccca
     2041 gggcatctgg gacgacccgg ccttctccct ctaccgccag gccgtcgagg ccatggacaa
     2101 gaaggactac gcgcgcgcca ccgatctgag cgggcaggcc atcgcccagc agccgacgca
     2161 cgtgctcgcc cactacctcc gtggccaggc cgcgatgtac cagtcgcgct gggacgacgc
     2221 ggccgctgcc ttcggcaagg tggtggagct ctaccccggc tccttcgcgg ggcatcgcga
     2281 cctgggtggc gcctacgagc agctcaaccg ggtggacgac gcggctcgcc agtacgaggc
     2341 ggccatcgcg ttgcgcgacc aggacgatct gcgcgtgcgg ctggcattca tgctgatcaa
     2401 ggccaaccag cagccccgcg ccctgcccca tctgcagacg ctggccgacc gcgactccaa
     2461 ggctcccgag gtgtggagcg ccctcgcccg cgtggcctac gacaagagcg atctgccggc
     2521 ggccgagaag aacttcaccc gcgcggctgc tctgcgcgac gacgggcgga cgtggttcaa
     2581 cctgggcgtg gtgcgcaccc gcctgaacga cctgcccggt gcgctccagg ccttcgagaa
     2641 ggcggcccag cacagcgaga cgaaggagcc ggcccagaag gaagtggcca aggtgcgcga
     2701 ggccatgaag cagccgacca ccgggaccgc gcccgatcgc gcgatgccgg gtagcacgcc
     2761 cggccagccg gtaccgactc gccgctacta gggtggccgt gtccatccgt cccgtcgtct
     2821 tctggctgca cctgctggtg ggcgtgaccg ccgggacggt gatcgccttc atgtcggtga
     2881 ccggcgtgct cctggccttc gagccgcaga tcaccgactg gctcgagcgc gaccgccgga
     2941 tcgtcgtgcc gccgcccggc gcgtcccgct tgcccgtgga gacgctggtg gcccgcgccc
     3001 gggaggcgcg gcccgacgtg cgcccctcca tcgtcacgct ccgcgccgac ccgacggccg
     3061 ccctcgtggt gagcttcggc cgcgacggcg gcgcgctctt catcgatccc taccgcggca
     3121 ccgtgctggg cggcttctcc cgggtgcacg acatgctcca cgaggtggtc gagtggcacc
     3181 ggtggctcgg ctcgcgagag atcgcccggc cgatcaccgg cgccgccaac ctgggcttcc
     3241 tgggactggt ctcgctcggc gccttcctct ggtggccccg gtgctggacc agcgcggcgc
     3301 tccggcaggt cactcgcttc gatgcgagcc tgcgtggccg ggc
//
LOCUS       DATMCV010000623         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_969825, whole genome shotgun sequence.
ACCESSION   DATMCV010000623 DATMCV010000000
VERSION     DATMCV010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969825"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VNU03_14390"
     CDS             complement(<1..773)
                     /locus_tag="VNU03_14390"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP75707.1"
                     /translation="MRSPSAPSIRHDLVLVGGGHAHIQVLKRWAMAPVPGARLTVVVD
                     RPIAVYSGMVPGFVAGQYARDALEIDVRPLALRAGARCIVAAATGVDPAARRLLLDGR
                     PPITYDTVSFDVGSTVAGLEIPGAREHAIPTRPIGEFVRRVDPILIAARGRSTFRMIV
                     VGAGAGGVEVAFALTARLRGEPGRRVDVLLLEAGPRVLPGYAASAARRVKAAADARGI
                     TIRCGAAVARVEAGAVHLVGGERLAADATVWVTGAAALP"
     gene            complement(795..>2243)
                     /locus_tag="VNU03_14395"
     CDS             complement(795..>2243)
                     /locus_tag="VNU03_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWP75708.1"
                     /translation="AMKAGEIFVAGKWQATRSGETYQPINPANEEPIGAVGKGDEGDI
                     DLAVTAARKAFDEGPWPKMNPHERGRIVWRLGDLIQQNLDEMARLESLCTGKTMFDSG
                     KVEIPFAAEVFRYYAGWATKIHGETLGLRDNAFTFTLRQPVGVVGAIVPWNFPFLLSS
                     WKIAPALAAGNTMVIKPASQTPLTALRFAEICQEAGVPEGVFNVVTGPGGKVGMALVR
                     DPRVDKIAFTGSTDVGKQIMREAAGTLKRLSLELGGKSPNIVFADADMDAAVRGALTG
                     IFYNKGEVCAAGSRLFLEDSIHDQFMSKLTDRVKSLKVGDPMDKATRMGPVVSKGQME
                     TVLSYIESGKQEGARVVAGGGRADVGTGKGYFIQPTIFDGVTNEMKIAREEIFGPVLS
                     VISFRSVEDGIAQGNHTSYGLAAAVWTRDVSKALKAAKAIRAGTVWVNAYNLFDAALP
                     FGGFKESGFGREMGSVGLDNYTEVKTVWVDLS"
BASE COUNT          315 a          806 c          799 g          323 t
ORIGIN      
        1 atcggcaggg cggcggcgcc ggtcacccac acggtggcgt cggccgcgag gcgctcgccg
       61 cccaccagat gcaccgcgcc ggcctccacg cgcgccaccg cggcgccgca acggatcgtg
      121 atgccgcgcg cgtccgccgc ggccttgacc cgacgggccg cgctcgccgc gtagcctggg
      181 aggacgcgcg gccccgcctc gagcaggagg acgtcgacgc ggcggcccgg ctcgcctcga
      241 agccgcgcgg tgagggcgaa ggcgacctcc acgccgcccg cgccggcgcc gaccacgatc
      301 attcggaagg ttgatcgtcc gcgcgcggcg atcaggatcg ggtcaacgcg gcggacgaac
      361 tcgccgatgg gccgcgtggg gatcgcgtgc tcgcgcgcgc ccgggatctc gagcccggcg
      421 accgtcgagc ccacgtcgaa cgacacggtg tcgtaggtga tgggcggccg gccgtcgagc
      481 aggagccggc gcgcggccgg gtcgacaccg gtggccgcgg ccacgatgca gcgggcgccc
      541 gcgcgcaggg ccagcgggcg cacgtcgatc tcgagggcgt cgcgggcgta ctggccggcc
      601 acgaagccgg ggaccatgcc ggagtagacc gcgatcggcc gatccactac gacggtcagg
      661 cgtgccccgg gtaccggggc catggcccag cgcttgagca cctggatgtg ggcgtgaccg
      721 ccgcccacca ggaccaggtc gtgccggatg ctaggagcgg acggcgagcg catggcccgt
      781 ccgcggtggt gtggctacga caggtcgacc cagacggtct tgacctcggt gtagttgtcg
      841 aggcccaccg accccatctc gcggccgaag cccgactcct tgaagccgcc gaacggcagc
      901 gccgcgtcga agaggttata ggcgttgacc cacaccgtgc ccgcccggat cgccttcgcc
      961 gccttcaggg ccttggagac gtcgcgcgtc cacaccgcgg cggccaggcc gtaggacgtg
     1021 tggttgccct gggcgatgcc gtcctccacc gagcggaagg agatcaccga gagcaccggg
     1081 ccgaagatct cctcgcgggc gatcttcatc tcgttggtga cgccgtcgaa gatggtgggc
     1141 tggatgaagt agcccttgcc ggtgccgacg tccgcccggc cgccgcccgc caccacccgc
     1201 gcgccctcct gcttgccgga ctcgatgtag gagagcacag tctccatctg gcccttcgac
     1261 accacggggc ccatgcgggt ggccttgtcc atcgggtcgc cgaccttcag gctcttgacc
     1321 cggtcggtca gcttggacat gaactggtcg tggatcgagt cttccaggaa gagccgcgag
     1381 ccggccgcgc acacctcgcc cttgttgtag aagatgccgg tgagcgcgcc gcgcaccgcc
     1441 gcgtccatgt ccgcgtcggc gaacacgatg ttgggcgact tgccgccgag ctcgagcgag
     1501 aggcgcttga gcgtccccgc cgcctcgcgc atgatctgct tgccgacgtc ggtggagccg
     1561 gtgaaggcga tcttgtcgac ccgcgggtca cgcaccagcg ccatgccgac cttgccgccg
     1621 ggcccggtga ccacgttgaa cacgccctcc ggcacccccg cctcctggca gatctcggcg
     1681 aagcgcagcg cggtgagggg ggtctgcgac gcgggcttga tcaccatcgt gttgccggcg
     1741 gccagcgcgg gggcgatctt ccaggacgag agcaggaacg ggaagttcca gggcacgatg
     1801 gccccgacca ctcccaccgg ctggcgcagc gtgaaggtga aggcgttgtc gcgcaggccg
     1861 agcgtctcgc cgtggatctt ggtggcccag cccgcgtagt agcggaagac ctccgccgcg
     1921 aacgggatct cgaccttgcc cgagtcgaac atggtcttgc cggtgcagag gctctcgagc
     1981 cgggccatct cgtcgaggtt ctgctggatc aggtcgccga ggcgccagac gatgcgcccg
     2041 cgctcgtgcg ggttcatctt cggccacggg ccctcgtcga aggccttgcg ggcggcggtc
     2101 accgccaggt cgatgtcgcc ctcgtcgccc ttgccgaccg ccccgatcgg ctcctcgttg
     2161 gccgggttga tcggctggta cgtctcgccc gaccgcgtgg cctgccactt ccccgcaacg
     2221 aagatctcgc ctgccttcat ggc
//
LOCUS       DATMCV010000624         2735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_300374, whole genome shotgun sequence.
ACCESSION   DATMCV010000624 DATMCV010000000
VERSION     DATMCV010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300374"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VNU03_14400"
     CDS             <1..628
                     /locus_tag="VNU03_14400"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75709.1"
                     /translation="VSDPVGLGFVQSLARPGGNITGFMQWPHSLWGKRIELLTELLGR
                     PPRRLAWIGNPGNAGSPNNWTDAREAAGRVGSEIIRIDVNGAGDLERAFDAAKGRDAL
                     LVQFDFLFAVERQRVAALAVRQRLPAIYENRMQALGGGLISYGGDLRENYRQGAAYVH
                     RVLNGATVGHLPVVQASRFELVINVPAARALGLRVPERLLVRADELIE"
     gene            695..1741
                     /locus_tag="VNU03_14405"
     CDS             695..1741
                     /locus_tag="VNU03_14405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75710.1"
                     /translation="MHFGIFLEERRRGVSESATLRETIDLADTGEAQGLDGIWLGEIH
                     FNGSRSIQSAPLAFASFLAARTRRLRIGIAVQVLPLGNPLRIAEEAATVDQLSEGRLD
                     FGVGRSGSARTYDLLGVPYGESQARFLECLAIIREAWKGQPFSYEGQFYRFPTVTVAP
                     TPYQVPHPPMRMAANSPETFPQVAKLGLPLFIGLRDLDIPLLRGHLKAYREAWRESGQ
                     AGPADVFLRIPVYAAPTEQAAREEPRAAMTYFFERHVELVRSGLGRAGAGPGDRRQAM
                     ADRIVNMSYDEILKTRVAFGTAPALRDRLGEIAEDLGLAGIVAELNPSGLLSLDQMKR
                     TLDILTREVIPTFR"
     gene            complement(1728..2492)
                     /locus_tag="VNU03_14410"
     CDS             complement(1728..2492)
                     /locus_tag="VNU03_14410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWP75711.1"
                     /translation="MCVDDDSFPPIRPIAGGAAGSRDFHLTSADGTRVMAHVARAAKP
                     TGAGMVVIPDVRGLHPYYKELADRFAEIGVDSVAIDFFARTAPSEDRSEKFDFMAQVP
                     LTKPETLQSDIAAAAAYLRTPDGGQVKSLYSVGFCFGGALSYLQAASGLDYAGVIGFY
                     GWPSGLKRWPDRPKPVDAVPRYKCKVQSIFGGADEGIPPADVEGFDAACRKAGIKHDM
                     KVYDGAPHSFFDRKYTEFADASADAWKRVQAFVSGK"
     gene            complement(2520..>2735)
                     /locus_tag="VNU03_14415"
     CDS             complement(2520..>2735)
                     /locus_tag="VNU03_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP75712.1"
                     /translation="GLTINTVTPGFIATEMLEHVPAKVLEKIVGQIPLGRLGKPEEVA
                     RVVHFLAADSSSYITGQVWGVNGGLDM"
BASE COUNT          391 a          980 c          959 g          405 t
ORIGIN      
        1 tgtgtccgat cccgtcgggc tgggctttgt tcaaagcctc gcgcggcccg gcggcaacat
       61 cacggggttc atgcagtggc cccactccct ctggggcaag cgcatcgagc tgctgaccga
      121 gctgctgggg cgcccgccgc ggcgcctcgc ctggatcggc aatcccggca acgcgggcag
      181 cccgaacaac tggaccgacg cccgggaggc ggcgggccgg gtcggcagcg agatcatccg
      241 catcgacgtc aacggggcgg gcgacctcga gcgggcgttc gacgcggcca agggccgcga
      301 cgcgctgctc gtgcagttcg acttcctgtt cgcggtggag cggcagcgag tggcggctct
      361 cgcggtgcgg cagcgcttgc ccgcgatcta cgagaaccgg atgcaggcgc tcgggggcgg
      421 gctcatctcg tacggaggcg atctccgcga aaactaccgg cagggcgccg cgtacgtcca
      481 ccgcgtgctc aacggggcga cggtcggcca tctgccggtg gtccaggcga gccggttcga
      541 gctggtgatc aacgtcccgg ccgcgcgagc cctcggcctc cgcgtccccg agaggctgct
      601 cgtgcgcgcc gacgagctga tcgagtgacc gccgacgtca cttgacgacg tggggcgcgc
      661 ggcgcaagat gcgcgggcgg aggaaaccgg gcccatgcac ttcgggatct ttctcgagga
      721 gaggcgccgc ggcgtcagcg agagcgcgac gctgcgcgag accatcgatc tggccgacac
      781 cggggaggcc caaggcctcg acgggatctg gctcggcgag atccacttca acggctcgcg
      841 ctcgatccag tcggctcccc tcgcgttcgc cagcttcctg gccgcgcgca cccgccgcct
      901 ccggatcggg atcgcggtgc aggtgctccc gctcggcaac cccctgcgga tcgcggagga
      961 ggcggccacc gtcgaccagc tcagcgaggg ccgcctcgac ttcggcgtcg gccgcagcgg
     1021 ctccgcccgc acctacgatc tgctcggcgt gccctacggg gagagccagg cccgcttcct
     1081 cgagtgcctc gcgatcatcc gggaggcctg gaagggccag cccttcagct acgagggcca
     1141 gttctatcgc ttccccaccg tgaccgtcgc gcccacgccc taccaggtgc cgcacccgcc
     1201 gatgcgcatg gccgccaact cgccggagac cttcccgcag gtcgcgaagc tcggcctgcc
     1261 gctgttcatc gggctgcgcg atctggacat cccgctgctg cgcggccacc tcaaagcata
     1321 ccgcgaggcg tggcgcgagt ccgggcaggc gggccccgcg gacgtcttct tgcgcatccc
     1381 ggtctacgcg gcgccgaccg agcaggccgc ccgcgaggag ccgcgggcag cgatgacgta
     1441 cttcttcgag cggcacgtgg agctggtccg ctccgggctc gggcgcgccg gcgccggtcc
     1501 cggtgaccgc cggcaggcca tggccgaccg catcgtcaac atgtcgtacg acgagatctt
     1561 gaagacgcgg gtcgcgttcg gcaccgcgcc cgcgctccgg gaccgcctgg gcgagatcgc
     1621 ggaggacctg gggctcgccg gcatcgtcgc cgagttgaac cccagcggcc tcctctcact
     1681 cgatcagatg aagcggacgc tcgacatcct gacccgcgag gtgatcccta ctttccgctg
     1741 acgaaggcct gcacgcgctt ccacgcgtcg gccgacgcgt ccgcgaactc ggtgtacttc
     1801 cggtcgaaga agctgtgcgg cgccccgtcg tacaccttca tgtcgtgctt gatccccgcc
     1861 ttgcggcacg ccgcgtcgaa gccctccacg tcggcgggcg ggatgccctc atcggctccc
     1921 ccgaagatcg actggacctt gcacttgtag cgcggcaccg cgtcgaccgg cttgggacgg
     1981 tccggccagc gcttgagccc cgacggccag ccgtagaagc cgatcacccc cgcgtagtcg
     2041 agcccgctcg cggcctgcag gtaggagagc gccccgccga agcagaagcc caccgagtag
     2101 agcgacttca cctgcccgcc gtcgggcgtc cggaggtagg ccgcggcggc cgcgatatcg
     2161 gactggagcg tctccggctt cgtcagcggc acctgggcca tgaagtcgaa cttctcgctg
     2221 cggtcctcgc tgggcgcggt gcgcgcgaag aagtcgatcg cgaccgagtc gacgccgatc
     2281 tcggcgaagc ggtcggccag ctccttgtag tacgggtgca ggccgcgcac gtccgggatc
     2341 accaccatgc ccgcgccggt gggcttggcc gcgcgggcca cgtgggccat gacccgggtg
     2401 ccgtcggcgg aggtcaggtg gaagtcacgc gagccggccg cgccgcccgc gatcgggcga
     2461 atgggaggaa aggaatcgtc gtccacgcac atgatcggat ctcctgttgg ttgggttcgt
     2521 tacatgtcca ggccgccgtt gaccccccac acctggccgg tgatgtagct cgacgagtcg
     2581 gcggccagga agtgcacgac ccgcgcgacc tcctcgggct tgccgagccg gccgagcggg
     2641 atctgcccga cgatcttctc cagcaccttg gccgggacgt gctccagcat ctcggtggcg
     2701 atgaagccgg gggtcacggt gttgatggtg aggcc
//
LOCUS       DATMCV010000625         4986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_100810, whole genome shotgun sequence.
ACCESSION   DATMCV010000625 DATMCV010000000
VERSION     DATMCV010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100810"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..198)
                     /locus_tag="VNU03_14420"
     tRNA            complement(124..198)
                     /locus_tag="VNU03_14420"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Gly,seq:ccc)
     gene            complement(251..1786)
                     /locus_tag="VNU03_14425"
     CDS             complement(251..1786)
                     /locus_tag="VNU03_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75713.1"
                     /translation="MLVKRLSALLIALLVAPGLSWAAPEGKVVIAQGVDPSTLDTMNQ
                     QETPASVVAAHIFDTLVERDQNLKAVPALAAEVPKLVAPTVWEVKLRRGVKFHNGEEF
                     NAESVKYSLERVKTGLRASSNFRPIDHVEIVDPYTVKVHTAKPWPTFVSIMTFRQASM
                     YPPKAYAGKDSAFISKNPIGTGPYKLVRWSKDEEIVLEANDQYWRGAPKIKTVVFRPI
                     PDDAVRVAALQNGEVDVAVNIPPHLASIIAGHPKVFLSTAPSIRTLQLMFVTHEFDAQ
                     HKLVGPYKGVTTDKRVRQAIAYALDVDEIIKGVLDGKAMRTATLLTPLHFGYDASLKP
                     IKQDLGKVKQLLTEAGFPGGLELTLNSPQGRYVRDKEVAEAVAGQLTKAGIKTQLKTY
                     EFVSYLNNLVYAHKPGPVWLIGWGTPTVDAETVYGPLFRTGSNLGNYSNPDFDGMVDQ
                     AQTEMNEKQRLAIYHRINKLWIDDMPAVPLYQQLDLYGASKRLNWKARSDELIKAYDM
                     SLK"
     gene            complement(2001..3467)
                     /locus_tag="VNU03_14430"
     CDS             complement(2001..3467)
                     /locus_tag="VNU03_14430"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWP75714.1"
                     /translation="MAPRVALAAIPESGRRTLGLLVELGGADRPLWLVGGAVRQLLAA
                     EPLDDLDVAVRSGALALGRQLADRMGASFVVLDESRGAGRIVGAEVGLPVDLVDFRAP
                     TLEGDLRARDFTVNALAVPLADVLRDGSAEVTDATGGVGDLGDRVVRLCGPSAIVDDP
                     VRALRGVRLAMREGWRLEPGTEAAIRTGAPLLAHVSAERVRDELVGILGEPRAAAGLR
                     MLDRLGALTVLLPESAAMRATSQPLPHRFDVWEHSIRTVEGVDLLLSDLDALAPWGVA
                     LRAHLGEALGGGISRRETLRLAALLHDVAKPETRTEQGGRVHFFGHDAAGALRTGAIA
                     ERLRLPRRASQVLERLVGEHLRPMHLAQAGQITRRARFRFFRDLGEDASDLLLLALAD
                     AAALTGGSPLAVWVAEGGTVVRELMAGAEEAVAAATTPPLLRGQEVMEAFHLPPGPAV
                     GRLLGRAREAQALGLVTTREEALDYLRRGGVADADADA"
     gene            complement(3472..4323)
                     /locus_tag="VNU03_14435"
     CDS             complement(3472..4323)
                     /locus_tag="VNU03_14435"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75715.1"
                     /translation="MATAPPGFRYIDIHTHLHPPWLWKAIRRWFEGRAGWEFRYPTDP
                     DLVARFLGEQGVERFVFFSYAHKPGLARELNGWLHETAGRLPTGLALGTVHPGDPDVL
                     DVAREALDRYGFKGFKFHINVQRFHPDDPRILPVYELLLARGALLLIHVGSAPWPNEY
                     DGFPRFERVMAMFPEMKVIVAHMGQWETRRHLELMPRCPNMYLDTTAAMAAQSPIYLG
                     GGGASPADVTDEDLVRWQDRVLFGSDFPNTPHPYEEERRPIWERTLPSAVHRKIFFDN
                     ARRLLDL"
     gene            complement(4323..>4986)
                     /locus_tag="VNU03_14440"
     CDS             complement(4323..>4986)
                     /locus_tag="VNU03_14440"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWP75716.1"
                     /translation="PRWPRRSHHDAGIGLTWELKDVLPARRQVYYGKLVKGRPMLVAL
                     DLFPVFYALVRGRQHARDYRREYEEGRLSATAKRIMDRLVRESPQYTRGLRAECFMLE
                     AAKTREFERAMGELQQGLWIVKTEERYEPTFSYRWDLVERWLPDEVAAGRRLRRPAAL
                     ERLLARYLAGAVYSTPALLARLFGLGRDEVQVALDALARAGAVETGVEVAGWPGRFVV
                     HA"
BASE COUNT          734 a         1801 c         1707 g          744 t
ORIGIN      
        1 ggacggctca gcgatcgcgc ggctgatgaa agatcggggc ttctgatggg cagcatgggc
       61 accggatggg caccggacgt gcgcaggggg ccacggcgct gcaccgtaac cccccgcgaa
      121 aattggagcg gccgaccgga ttcgaaccgg cgacgtccag cttgggaagc tggcattctg
      181 ccactgaatt acggccgctc gtgcccccta ttctagtctt cgcccccgcc ctgtcaaact
      241 cggtgggaat ctatttgagc gacatgtcgt aggccttgat cagttcgtcg ctgcgggcct
      301 tccagttgag ccgcttgctc gccccgtaga ggtcgagctg ctggtagagc ggcaccgccg
      361 gcatgtcgtc gatccagagc ttgttgatcc ggtggtagat ggccagccgc tgcttctcgt
      421 tcatctcggt ctgcgcctgg tccaccatgc cgtcgaagtc cgggttgctg tagttgccga
      481 ggttgctgcc ggtccggaac agcggcccgt agaccgtctc cgcgtccacc gtgggcgtgc
      541 cccagccgat cagccagacc gggccgggct tgtgcgcgta caccaggtta ttcaggtagc
      601 tcacgaactc gtaggtcttg agctgggtct tgatgccggc cttcgtgagc tgcccggcca
      661 ccgcctccgc cacctccttg tcgcgcacgt agcggccctg cgggctgttg agggtcagct
      721 cgaggccgcc cgggaagccg gcctcggtca ggagctgctt gaccttgccg aggtcctgct
      781 tgatcggctt gagcgacgcg tcgtagccga agtgcagcgg ggtgagcagc gtggcggttc
      841 gcatcgcctt gccgtcgagc acccccttga tgatctcgtc gacgtcgagc gcgtaggcga
      901 tggcctgccg cactcgcttg tccgtggtca cgcccttgta cggccccacc agcttgtgct
      961 gggcgtcgaa ctcgtgggtg acgaacatca gctgcagcgt ccggatggac ggcgcggtcg
     1021 agaggaacac cttgggatgg cccgcgatga tgctggcgag gtgcggcggg atgttcaccg
     1081 ccacgtccac ctccccgttc tgcagcgcgg ccacccgcac cgcgtcgtcg gggatgggcc
     1141 ggaacaccac cgtcttgatc ttgggggcgc cccgccagta ctggtcgttg gcctcgagca
     1201 cgatctcctc gtccttgctc cagcggacga gcttgtaggg cccggtgccg atcgggttct
     1261 tcgagatgaa cgcgctgtcc ttgcccgcgt aggccttggg cggatacatc gacgcctggc
     1321 ggaaggtcat gatcgacacg aacgtcggcc acggcttggc ggtgtgtacc ttcacggtgt
     1381 acgggtcgac gatctcgacg tgatcgatcg ggcggaagtt cgagctggcg cgcaggccgg
     1441 tcttgacccg ctccaggctg tacttcaccg actcggcgtt gaactcctcg ccgttgtgga
     1501 acttgacccc tctgcgcagc ttcacttccc acaccgtggg ggccaccagc ttcggcacct
     1561 ccgcggccag ggccggcacg gccttgaggt tctgatcgcg ctccaccagc gtgtcgaaga
     1621 tgtgcgccgc caccaccgag gcgggcgtct cctgctggtt catggtgtcg agcgtgctgg
     1681 gatccacccc ctgggcgatg acgaccttgc cctcgggggc cgcccacgac agccccggcg
     1741 ccacgagcag ggcgatcagg agcgcactga gacgtttcac gagcattggg atcctccttg
     1801 gagccgggac attgcgggga aagtggtcac actctaggca accgcgggct ccggtgtcaa
     1861 gttccggagg ccgtcaacgc gcctcgcgca gtcccatctc gtagcacttg atcagctcgt
     1921 tgccccacgc cttcgacccc agtcggcggc tcgcgccgtg ctcaggaatc ggccctctct
     1981 agggacgcct cgcggggaag tcacgcatcg gcatcggcat cggctacgcc gccgcgccgc
     2041 aggtagtcga gggcctcctc tcgcgtggtc acgagcccca gggcctgcgc ctcccgcgcc
     2101 cgaccgagga gacgtccgac cgccggaccg ggcggcagat ggaaggcctc catcacctcc
     2161 tgcccacgca ggagcggggg cgtggtcgcg gcggccaccg cctcctccgc gccggccatc
     2221 agctcgcgca ccaccgtccc gccctccgcg acccacaccg ccaggggcga gccgccggtg
     2281 agcgcggccg cgtccgccag cgcaaggagc aggagatcgg acgcgtcctc gccgagatcg
     2341 cggaagaagc gaaagcgtgc tcgccgggtg atctgcccgg cctgggccag gtgcatcggg
     2401 cggaggtgct cgcccacgag ccgctccagg acctggctcg cccgccgggg gagccggagg
     2461 cgctcggcaa tggcgccggt ccggagcgcg ccggccgcgt cgtgtccgaa gaagtggact
     2521 cgcccgccct gctccgtgcg ggtctcgggc ttggcgacgt cgtggagcag cgcggccagc
     2581 cggagcgtct cccgccgcga gattccgccg cccagggcct cgcccaggtg cgcccgcagc
     2641 gccacgcccc acggggcgag cgcgtcgaga tcggagagca gcaggtccac gccctcgacc
     2701 gtccggatcg aatgctccca gacgtcgaac cgatgcggca gcggctgcga ggtggcgcgc
     2761 atcgccgcgc tctcgggtaa cagcaccgtc agcgccccca ggcgatccag catccggagt
     2821 ccggccgcgg cgcgcggctc gcccaggatg cccaccagct cgtctcgcac ccgctctgcg
     2881 gagacgtgcg cgaggagtgg cgcgcccgtc cggatggcgg cctccgtccc cggctcgagc
     2941 cgccagccct cccgcatggc cagacgcacg ccgcgcaggg cccgcaccgg atcgtcgacg
     3001 atcgcgctcg ggccgcagag gcgcaccacg cgatcgccga ggtcccccac gccaccggtc
     3061 gcatccgtga cctccgcgct gccgtcccgc aacacatccg ccagcggcac cgcgagcgcg
     3121 ttgaccgtga agtccctcgc ccgcaggtcg ccctcgagcg tgggggcgcg gaagtcgacc
     3181 aggtcgaccg ggagccctac ctccgcgccg acgatgcggc cggcgccgcg gctctcgtcg
     3241 agcaccacga agctcgctcc catccgatcc gcgagctgcc gtccgagcgc cagggctccg
     3301 cttcgcaccg ccacgtccag gtcgtccagc ggctccgcgg ccagcagctg ccggaccgct
     3361 ccgcccacga gccacagcgg acggtccgcg ccgcccagct cgaccagcag gccgagcgtc
     3421 cgccggccgg actcgggaat ggccgccagc gcgacgcgag gagccatccg gctacagatc
     3481 gagaagacgc cgggcgttgt cgaagaagat cttccggtgg accgccgacg gcaaggtccg
     3541 ctcccagatc gggcggcgct cctcctcgta cgggtgggga gtgttcggga agtcgctccc
     3601 gaagagaacg cggtcctgcc agcgcacgag atcctcgtcg gtgacgtcgg ccgggctcgc
     3661 tccaccgccg cccagataga tcgggctctg ggccgccatc gcggcggtgg tgtcgaggta
     3721 catgttggga cagcggggca tcagctcgag atgccgccgc gtctcccact ggcccatgtg
     3781 ggccacgatc accttcatct ccgggaacat cgccatcacg cgctcgaagc gggggaagcc
     3841 gtcgtactcg ttcggccagg gcgcgctccc cacgtggatc aggagcagag cgccgcgggc
     3901 cagcagaagc tcgtacaccg gcaggattcg cgggtcgtcg gggtggaagc gctgcacgtt
     3961 gatgtggaac ttgaagccct tgaacccgta gcgatcgagg gcctcgcgcg cgacgtcgag
     4021 cacgtccggg tcgccggggt gcacggtacc gagcgcgagc ccggtgggca gccgcccggc
     4081 cgtctcgtgc agccacccgt tcagctcgcg cgcgagcccc ggcttgtgcg catacgagaa
     4141 gaagacgaac cgctccacgc cctgctcgcc gaggaagcgg gccaccaggt ccggatccgt
     4201 cggataccgg aactcccacc cggcccggcc ctcgaaccag cggcggatcg ccttccacag
     4261 ccacggcggg tgcaggtgag tgtggatgtc gatgtagcgg aatcccggag gagcggtcgc
     4321 catcaggcgt gcaccacgaa gcgccccggc cagcccgcga cctcgacccc ggtctccacc
     4381 gcgcccgcac gagcgagcgc gtcgagggcg acctggacct cgtcgcgccc gaggccgaag
     4441 agccgcgcca ggagggccgg cgtcgagtag accgcgccgg ccaggtaccg cgcgagcagc
     4501 cgctcgagcg cggcaggtcg gcgcagccgg cggcccgcgg ccacctcgtc gggcagccag
     4561 cgctcgacaa ggtcccagcg atacgagaag gtgggctcgt agcgctcctc ggtcttgacg
     4621 atccacagcc cctgctgcag ctcacccatc gcgcgctcga actcccgggt cttggccgcc
     4681 tccagcatga agcactccgc gcgcagaccc cgcgtgtact gcggcgactc gcgcaccagg
     4741 cggtccatga tgcgcttggc ggtcgcggac aggcgtccct cctcgtactc gcgtcgataa
     4801 tcccgggcgt gctggcggcc ccgcacgagg gcgtagaaga ccgggaagag gtcgagcgcc
     4861 accagcatcg gacggccctt gaccagcttg ccgtagtaga cctgacggcg ggcgggcagc
     4921 acgtccttca gctcccaggt gagcccgatc cccgcgtcgt ggtgcgagcg gcgcggccac
     4981 cgcggg
//
LOCUS       DATMCV010000626         5807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_31008, whole genome shotgun sequence.
ACCESSION   DATMCV010000626 DATMCV010000000
VERSION     DATMCV010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31008"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VNU03_14445"
     CDS             complement(<1..246)
                     /locus_tag="VNU03_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWP75717.1"
                     /translation="MAQRVFLTGSTGFVGKHVARALLARGFLVRCLVRPGSEHELKGF
                     ESIDRVPGDVMEPDDLAPSAEGCAAILHLVGIIREQPS"
     gene            317..2662
                     /locus_tag="VNU03_14450"
     CDS             317..2662
                     /locus_tag="VNU03_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HWP75718.1"
                     /translation="MRCRAASLLVLLLLISSTPAAATAQPAAWTQAADEAVRDSVAAS
                     EVPGAVLVVGQGDQILHRKVLGWRATVPHPELMTADTIFDIASLTKVVATTPSVLRLW
                     EMGKVDLNAPLGQYLKEFNTAAFQDVTVARLLTHSAGMPDLPSREAMAKGFPEAARLQ
                     AKAGLAVAPGTTFLYSDTGFILLGELVRRVSGEPLDRFAQRNFYTPLGMRDTAFAPPQ
                     SWVKRIAPTEVVNAHGPLRGTVHDGNARLLHGVAGHAGVFSTATDLSRYVRMLLSGGA
                     LGGKRYLKEATVRAMFSPHVIGESTRGLGWDIASPYSRTLGAYFPMGSVGHTGFTGTA
                     IWMDPPSQVYMILLTNRVHPYGKGDVAELRRRISAAVGQRFGSRDEPPAAPPDSESPV
                     AAPAADAPARAEGPTLTGLDRLVADDFALLAGRSIGLITNQTGVDGQGRRGVDLLAAA
                     PRVRLRALFSPEHGITGQVDVNVPNSRDAATGLPIWSLYGPARRPSPEMLNGIDTLVF
                     DIQDVGVRYYTYLTTLVYALEEGGRRGIQVMVLDRPNPITGSVVEGPLMDPDMRSFTA
                     PHTIPVRTGMTIGEFAQMVVAERKLPVKLTVVPLERWQRGQWFDETGLPWVNPSPNIR
                     TPMQALLYSGVGLLEATNLSVGRGTDMPFEVIGAPWISDPQALADGMNARGLAGVRFQ
                     PASFTPTSSVYAGQAVGGVRLEVTDRDAIRPVSVGLALGRELMERYPSQFRPAAIQNL
                     LVNRTTMWSILRGEPFSRILGWADASRASFLQRRAPYLIYK"
     gene            complement(2679..3638)
                     /locus_tag="VNU03_14455"
     CDS             complement(2679..3638)
                     /locus_tag="VNU03_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWP75719.1"
                     /translation="MRTVVATVLLAVCLGAAVLAGAQAPTLVPNELGRVMILEYHKID
                     NPEARWTRTPENFKRDLTRLWERGYRTVALTDYLDGKIALPAGTSPVILTFDDSSPGQ
                     FRYIQKGADWVIDPACGVGILEAFAREHPGFGHAATFYVLPGAKAPNDLFNQADLAGR
                     KLQYLASQGYEIGNHTLWHAELGRYPEATVRDQLATAQAWVQRHVPGYRFRTVALPHG
                     SYPKELGWAVSGEAKGMTYKHDAILMVAGGAAPSPYAKNFDPLHLPRIQAVDKDFDYW
                     LTYFDRNPGERYVSDGDPTTVTVASGTTGKVGAVKAARVIERR"
     gene            complement(3641..5152)
                     /locus_tag="VNU03_14460"
     CDS             complement(3641..5152)
                     /locus_tag="VNU03_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HWP75720.1"
                     /translation="MSQSSVPRALSWTSLVALTLIAALAETTILFPSVLFPGTRLATA
                     LSWDASNPGYPLPEDPAAETSGTPATVAPSPAETPAVQASPAPAPAEPRGEPHVVVGR
                     VLDSATGNALRGVSIWAGASEALTDAEGRFTTKPVVPGTTIWVKAPGYERRKLTAGAA
                     EVTVKLPPHPIRAAYLTYYGVADRGIRQRVLDLVSRTELNAVVIDVKGDRGMIPYRTA
                     VQAAIDAGAQGPVIIKDFEEQLAAWKAQGIYTIARIVAFKDNLRATTRPDLAIIDTHT
                     GKPWIDRENLAWVDPFREENWDYLIAVAKEAVAKGFDEVQFDYVRFPTDGKLSAARYS
                     QPNNATTRLPAIAAFLAKARRELGPTGAFLGADVFGYTAFNSNDTDIGQRVEELSAHL
                     DYMCPMVYPSGYHLGIPGVRNPMKNPYEVVKESVRLTRQRSQNPAMQVRPWLQDFKDY
                     AFDKRIFGPGEVSAQIRGAGDAGASGWMLWNPKNDYTAAALKPKTSALTKSTQ"
     gene            5383..>5807
                     /locus_tag="VNU03_14465"
     CDS             5383..>5807
                     /locus_tag="VNU03_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP75721.1"
                     /translation="MTDPTRFGCEIARPRSLQVGGLALHALEWGEPGRPALCFLHGGS
                     AHAHWFDAVVPAFVDRYHVLSLDQRGHGASQWASEPAYATEDFACDLVGVMDALGWRR
                     MTVIGHSMGGHNAMAFAAWHPERVDRLVVVDSRPAIPAER"
BASE COUNT          795 a         2001 c         2106 g          905 t
ORIGIN      
        1 gctcggctgc tcgcgaatga tgccgaccag gtggaggatg gccgcgcagc cctcggcgct
       61 cggggcgagg tcgtcgggct ccatcacgtc gccggggacg cgatcgatcg actcgaagcc
      121 cttcagctcg tgctccgagc cgggccgcac cagacagcgg acgagaaatc cccgcgcaag
      181 aagtgcgcga gcgacgtgct tgccgacgaa gccggtgctg ccggtgagga atacgcgctg
      241 cgccacgccg cgaatcgtag catggccctc cgcgaatttc gcgccacggg tcggcggtga
      301 cctcgtatca tagggcatgc gctgtcgcgc agcctcgctc ctggtgctgt tgctgctgat
      361 ctcctccacg ccggcggcgg cgacggcgca gcccgcggct tggacgcagg cggcggacga
      421 ggcggtgcgc gactcggtcg ccgcctcgga ggtgccgggc gccgtcctcg tggtcggcca
      481 gggcgatcag atcctgcacc gtaaggtcct cggctggcgc gcgaccgttc cgcatccgga
      541 gctgatgacc gccgacacca tcttcgacat cgcctcgctc accaaggtgg tggccaccac
      601 gccctccgtc ctgcggctgt gggagatggg caaggtggac ctgaacgcgc cgctcgggca
      661 gtacctcaag gaattcaaca ccgccgcctt ccaggacgtc acggtggcgc ggcttctgac
      721 gcactcggcg ggcatgcccg atctgccctc gcgcgaggcc atggcgaagg gcttcccgga
      781 ggcggcgcgg ctgcaggcga aggccgggct cgcggtggcg cccggcacca cgttcctcta
      841 cagcgacacc gggttcatcc tgctcggcga gctggtgcgg cgcgtgagcg gcgagccgct
      901 cgaccgcttc gcccagcgca acttctatac cccgctcggc atgcgcgata ccgccttcgc
      961 gccgccccag tcgtgggtga agcgcatcgc gcccaccgag gtcgtgaacg cgcacgggcc
     1021 gctgcgcggg acggtgcacg acggcaacgc acgcctgctc catggtgtcg cgggccacgc
     1081 gggggtcttc tcgaccgcga ccgacctgag ccgctacgtc cgcatgctgc tgtcgggcgg
     1141 agcgctcggc ggcaagcggt atctgaagga agccaccgtg cgcgcgatgt tctccccgca
     1201 cgtgatcggc gagtcgaccc gcgggctcgg ctgggacatc gcctcgccct actcgcggac
     1261 cctcggcgcc tacttcccga tgggctcggt cgggcacacc ggcttcaccg gcaccgcgat
     1321 ctggatggat ccgccgagcc aggtctacat gatcctgctc accaaccggg tgcatcccta
     1381 tggcaagggc gacgtggccg agctgcgccg ccggatcagc gcggccgtcg gccagcgctt
     1441 cggctcgcgc gacgagccgc cggccgcccc gccggactcc gagagcccgg tcgccgcgcc
     1501 cgcggcggat gcgcccgccc gcgccgaggg ccccacgctg accggcctcg atcgcctggt
     1561 cgcggacgac ttcgcgctcc tcgcgggccg ctcgatcggg ctcatcacca accagaccgg
     1621 ggtggacggc cagggtcgcc gcggcgtgga cctgctcgcc gccgcgccgc gggtgcggct
     1681 tcgcgcgctg ttctcgcccg agcacgggat caccggtcag gtcgacgtca acgtcccgaa
     1741 tagccgcgac gcggccaccg ggctgcccat ctggagtctc tacggtccgg cccgccgccc
     1801 gtcgcccgag atgctgaacg gcatcgacac cctggtcttc gacatccagg acgtgggtgt
     1861 ccggtactac acgtacctga ccaccctcgt ctatgcgctc gaggagggcg gccgccgcgg
     1921 catccaggtc atggtgctgg accgcccgaa tccgatcacg ggcagcgtgg tcgagggccc
     1981 gttgatggat cccgacatgc gctcgttcac cgcgccgcac accattccgg tccggaccgg
     2041 gatgaccatc ggcgagttcg cccagatggt ggtggcggag cgcaagctgc cggtgaagct
     2101 gaccgtggtg ccgctcgagc gctggcagcg cgggcagtgg ttcgacgaga ccgggctgcc
     2161 gtgggtgaac ccgtcgccga acatccgcac cccgatgcag gccctcctct actcgggcgt
     2221 cggcctcctg gaggcgacga acctctcggt cgggcgcggc accgacatgc ccttcgaggt
     2281 gatcggtgcc ccatggatca gcgatcccca ggccctggcc gacggcatga atgcgcgagg
     2341 cctggccggt gtgcggttcc agccggcgtc cttcacgccg acctcgagcg tctacgcggg
     2401 ccaggcggtc ggtggggtac ggttggaggt gaccgaccgc gatgcgatcc gcccggtgtc
     2461 ggtcggcctc gcgctgggcc gggagctgat ggagcgctac ccgagccagt tccggccggc
     2521 ggccatccag aacctgctcg tgaaccggac cacgatgtgg tcgatcctgc ggggggagcc
     2581 cttctcgcgg atcctgggct gggccgacgc ctcgcgcgcc agcttcctgc agcgccgcgc
     2641 accctacctg atctacaagt aggcgcgcgc tccggcgctt accgccgctc gatcacccgg
     2701 gcggccttca ccgcgcccac cttcccggtg gtcccgctcg ccaccgtgac tgtggtgggg
     2761 tcgccgtcgc tcacgtagcg ctcgccgggg ttgcggtcga agtaggtgag ccagtagtcg
     2821 aagtccttgt cgaccgcctg gatgcggggc aggtggagcg ggtcgaaatt cttggcgtac
     2881 ggcgagggcg cggcgccgcc cgccaccatc aggatggcgt cgtgcttgta cgtcatgccc
     2941 ttggcttcgc cggagaccgc ccagcccagc tccttgggat agctgccgtg gggcagcgcc
     3001 acggtgcgga agcgatagcc ggggacgtgg cgctgcaccc acgcctgcgc ggtcgcgagc
     3061 tggtcgcgca cggtggcctc cgggtagcgg ccgagctcgg cgtgccagag cgtgtggttg
     3121 ccgatctcgt agccctgact cgccaggtac tggagcttgc ggccggccag gtccgcctgg
     3181 ttgaagagat cgttcggggc cttggcgccc ggcagcacgt agaacgtggc ggcgtggccg
     3241 aagccgggat gctcgcgcgc gaaggcctcg aggatgccga cgccgcacgc gggatcgatc
     3301 acccagtcgg cccccttctg gatgtagcgg aactggccgg gcgaggagtc gtcgaaagtg
     3361 aggatgaccg ggctggtgcc ggcgggcagc gcgatcttgc cgtcgaggta gtcggtgagg
     3421 gcgacggtgc gatagccgcg ctcccagagg cgggtcaggt cacgcttgaa gttctcgggc
     3481 gtgcgcgtcc agcgggcctc ggggttgtcg atcttgtggt actcgaggat catcacgcgc
     3541 ccgagctcgt tggggaccag cgtgggggcc tgggccccgg ccaggacggc cgcgcccagg
     3601 cagaccgcga gcaggacggt tgcgacgacg gtacgcatgg ctactgggtc gacttcgtga
     3661 gggcggaggt cttgggcttg agcgccgccg ccgtgtagtc gttcttcgga ttccagagca
     3721 tccacccgga ggcgcccgcg tcgccggcgc cgcggatctg ggcgctgacc tctcccggac
     3781 cgaagatgcg cttgtcgaag gcgtagtcct tgaagtcctg cagccacggc cgcacctgca
     3841 tggccgggtt ctgggagcgc tggcgggtga gccgcacgct ctccttcacg acctcgtacg
     3901 ggttcttcat cggattccgg acgcccggga tgcccaggtg atagccggac gggtacacca
     3961 tcgggcacat gtagtcgagg tgcgcggaga gctcctcgac gcgctgcccg atatcggtgt
     4021 cgttggagtt gaacgcggtg tagccgaaga cgtccgcgcc gaggaaggct ccggtggggc
     4081 ccagctcgcg acgagccttg gccaggaagg cggcgatggc gggcaggcgg gtggtcgcgt
     4141 tgttgggctg cgagtagcgc gcggccgaga gcttgccgtc ggtggggaag cgcacgtagt
     4201 cgaactgcac ctcgtcgaag cccttggcca ccgcttcctt ggccaccgcg atcaggtagt
     4261 cccagttctc ctcgcggaag ggatcgaccc acgcgaggtt ctcgcggtcg atccagggct
     4321 tgccggtgtg ggtgtcgatg atggcgaggt ccggccgcgt ggtggcccgc aggttgtcct
     4381 tgaaggccac gatgcgggcg atggtgtaga tgccctgggc cttccacgcc gcaagctgct
     4441 cctcgaagtc cttgatgatg accgggccct gcgcgcccgc gtcgatcgcg gcctgcaccg
     4501 cggtgcggta agggatcatc ccgcggtcgc ccttgacgtc gatgaccacc gcgttcagct
     4561 cggtgcgcga taccaggtcg aggacgcgct ggcggatgcc ccggtcggcg accccgtagt
     4621 aggtgaggta ggcggcgcgg atgggatgcg gcgggagctt cacggtgacc tcggcggccc
     4681 ccgcggtcag cttccgccgc tcgtagccgg gggccttcac ccagatcgtg gtgccgggga
     4741 cgacgggctt cgtggtgaac cggccctccg cgtcggtcag cgcctcgctg gcgcccgccc
     4801 agatcgacac gccgcggagc gcgttgccgg tggcggagtc gagcacacgg ccgaccacca
     4861 cgtgaggctc gccccgcggc tcggcaggag ccggagccgg cgaggcctgg accgcgggcg
     4921 tctccgccgg gctcggcgcg accgtcgccg gggtcccgga ggtctccgcg gccgggtcct
     4981 ccggcagcgg gtagccgggg ttgctggcgt cccacgagag cgcggtggcc aggcgggtgc
     5041 cggggaacag gaccgacggg aagaggatcg tggtctcggc gagggcggcg atcagcgtga
     5101 gggcgaccag cgaggtccac gacagcgcgc ggggtaccga gctttggctc attcgtcgtc
     5161 tttcctgact atcgcgttag gaggttggta gggccaaggg aattccgcga gacgacaaaa
     5221 tatgagcaac ctgcttgcca tctcggcgct ccggcaagtg actgaaaacg tggtcgccgg
     5281 ctcccggccc cgcgcccctc cctgtcggcg ggctgacaaa gtccgccagt tgacaacgcg
     5341 aatcgtcaca agcgagccaa gcccctgagt acaatgcggc gcatgaccga cccgacacgc
     5401 ttcggatgcg agatcgcccg tccgcgctcc ctccaggtcg gcggcctggc gctgcacgcc
     5461 ctcgagtggg gtgagcccgg ccggccggcg ctctgcttcc tccacggcgg ctccgcgcac
     5521 gcgcactggt tcgacgcggt cgtgcccgcc ttcgtcgacc gttaccacgt gctctcgctc
     5581 gaccagcgtg gccatggcgc gagccagtgg gcgtccgagc ccgcgtacgc caccgaggac
     5641 ttcgcctgcg acctggtcgg cgtgatggat gccctcgggt ggcgccggat gaccgtgatc
     5701 ggccactcca tgggcggcca caacgcgatg gcgttcgcgg cctggcaccc ggagcgcgtc
     5761 gatcggctgg tggtggtgga cagccgtccg gccatcccgg ccgagcg
//
LOCUS       DATMCV010000627         3266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_633382, whole genome shotgun sequence.
ACCESSION   DATMCV010000627 DATMCV010000000
VERSION     DATMCV010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633382"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VNU03_14470"
     CDS             <1..1121
                     /locus_tag="VNU03_14470"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWP75722.1"
                     /translation="RDLQQTQKQLRDERLKAADARKREASVLSELEAIDQRLTEKRKQ
                     VVAIDGRMRRAQADITDLQSEIGRLQTRRSGQEDVLGRRLRALYKLQAQGGVLPIVLS
                     GDDPVNQAVQLRHLTTLATVDARLIREYRVTSEVLADRKSRVEARGKELASLKSEAEQ
                     ERIEVDQEAAKRRALLSKVQDERAYHDRMVGELSEATRRLEAFIRDLQEKQRRAVAKV
                     PPPSRPARPSPGDAPTGTGFAALRGRLSWPADGRVVAEYGPQVNPRFGTKTFRNGIDI
                     EATEGSNIAAVFPGHVVYTGWFRGYGNLIIVDHGGEYYTVYAHAADIRVTEGDEVKQG
                     QLIGTVGDTGSLQGPRLYFEVRHEGKPQDPAQWLRPKG"
     gene            1141..2424
                     /locus_tag="VNU03_14475"
     CDS             1141..2424
                     /locus_tag="VNU03_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWP75723.1"
                     /translation="MRPIRHMKKVVLISSLLLILTLSLGGGVASKGNDTAATYENLRL
                     FTEVLSIVQSQYVDEVPPKDLIYNAIKGTLRGLDPHSSFLDPDMYREMQVETSGSFGG
                     LGIEITLRDDVLTVVAPIEGTPAYRAGIQPGDRILKIEGLSTKDMQLADAVKRMRGKP
                     GSKITVTIVREGWTEPKDFLITREQIRVQSVKNNQLEPGIEYIRLRQFQEQTSNDLET
                     ALEKYVKEGKIQGLVLDLRNNPGGLLTSAVEVTEKFLEPGKLVVYTEGRVRNQNMRFS
                     SNAKRSFTDFPVVVLVNQGSASASEIVAGALQDYGRAVVLGTQSFGKGSVQTIIPLSD
                     GSGLRLTTAKYFTPKGRSIHGKGITPDILVEGPKTTAVNTTGENPNPTPPVVETPQEQ
                     LKRDPQLQRALDLLKAMKILDKTRPTPTTPQAQAR"
     gene            2443..>3266
                     /locus_tag="VNU03_14480"
     CDS             2443..>3266
                     /locus_tag="VNU03_14480"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HWP75724.1"
                     /translation="MPARVWQALAGLIVLLLIAVVGLDQWQARRGEASLFGQGLLGSA
                     PSRSVAPAPSVVTRPSPPLEAAPAPPPPGAPRIAVIVDDLGGRRDVFDVLREIRRPLT
                     VAALPALPLSASIARDAFRSGMEVLLDVPMEPYRYPELDPGPGMLLTSMAPGEIQHAV
                     GTQLETVASAVGVMNRMGSRLTEDRTRMRAMLEVLAARRLFLVDAYTSSQSLAFDEAR
                     SAGVRAARRHILVDHTRGEAGDRLRWDEVAGWAERRGEVIVVAHGHPLTVKLLKEYV"
BASE COUNT          559 a         1090 c         1180 g          437 t
ORIGIN      
        1 agcgcgacct gcagcagacc cagaagcagc tccgggacga gcggctgaag gcggccgacg
       61 cgcgcaagcg cgaggcgagc gtgctgagcg agctggaggc gatcgaccag cggctcaccg
      121 agaagcgcaa gcaggtggtc gcgatcgacg ggcgcatgcg tcgcgcgcag gccgacatca
      181 ccgacctgca aagcgagatc ggccggctcc agacccgccg ctccgggcaa gaggacgtcc
      241 ttggccggcg cctccgcgcg ctctacaagc tgcaggccca gggcggggtg ctgcccatcg
      301 tgctctccgg cgacgacccg gtgaaccagg cggtccagct ccgccacctg accaccctgg
      361 ccacggtgga cgcgcgtctc attcgagagt atcgtgtgac gtccgaggtt ttggccgatc
      421 gcaagagccg ggtggaggcg cggggcaagg agctggcgtc gctcaagtcc gaggcggagc
      481 aggaacggat cgaggtcgac caggaagcgg cgaagcgccg cgccctgctg tcgaaagttc
      541 aggacgagcg ggcctaccac gatcggatgg tgggcgagct ctccgaggcc acccggcggc
      601 tcgaggcctt catccgagac ctgcaggaga agcagcggcg ggcggtggcg aaggtgccgc
      661 ccccgagccg gcccgcacga ccgtcgcccg gcgacgcgcc gacgggaacc gggttcgcgg
      721 cgctgcgggg acggctctcc tggccggcgg acggccgggt ggtcgccgag tatggcccgc
      781 aggtcaatcc acggttcggg acgaagacgt tccggaacgg gatcgacatc gaggccaccg
      841 agggctcgaa tatcgcggcg gtgttccccg gccacgtcgt gtacacggga tggttccggg
      901 ggtacgggaa cctgatcatc gtggatcacg ggggcgagta ctacacggtc tacgcccacg
      961 cggccgacat ccgggtcacg gagggcgacg aggtcaagca ggggcagctg atcggcaccg
     1021 tcggagacac cggctcgctg caggggccgc gcctgtactt cgaagtacgg cacgaaggca
     1081 agccacagga cccggcccag tggctgcgtc caaaaggata gttcaacgga ggcgtacacg
     1141 atgcggccca tccggcacat gaagaaggtg gtcctgatct cctcgctcct gctcatcctg
     1201 accctgtcgc tcgggggcgg ggtggcgagc aagggcaacg acaccgcggc cacctacgag
     1261 aacctgcggc tcttcaccga ggtgctctcg atcgtccaga gccagtacgt ggacgaggtc
     1321 ccgcccaagg acctcatcta caacgcgatc aaggggactc ttcgagggct ggacccgcat
     1381 tcctcgttcc tcgatcccga catgtaccgg gagatgcagg tcgagacgag cgggtccttc
     1441 ggcgggctcg gcatcgagat cacgctgcgc gacgacgtgc tgacggtggt cgcgcccatc
     1501 gagggcacgc ccgcctaccg tgcgggcatc cagcccggcg accgcattct aaagatcgag
     1561 ggcctctcca ccaaggacat gcagctggcc gacgcggtca agcgcatgcg gggcaagccg
     1621 ggcagcaaga tcacggtcac catcgtgcgc gagggctgga ccgagccgaa ggacttcctg
     1681 atcacccgcg agcagatccg ggtgcagtcg gtgaagaaca accagctcga gccgggcatc
     1741 gagtacatcc ggctgcgcca gttccaggag caaacctcga acgacctgga gaccgcgctg
     1801 gagaagtacg tcaaggaggg caagatccag ggcctggtgc tcgacctgcg caacaatccg
     1861 ggcggcctgc tcacctccgc ggtcgaggtg accgagaagt tcctcgagcc gggcaagctg
     1921 gtcgtctaca ccgaggggcg cgtgcgcaac cagaacatgc gcttctcctc gaacgccaag
     1981 cgctccttca ccgacttccc ggtggtggtg ctggtcaacc agggcagcgc ctccgcctcc
     2041 gagatcgtgg ccggcgcgct acaggactac gggcgcgcgg tcgtgctggg cacccagagc
     2101 ttcggcaagg gctcggtgca gaccatcatc ccgctctccg acgggtccgg tctgaggctc
     2161 accaccgcca agtacttcac cccgaagggc cggtcgatcc acggcaaggg catcaccccc
     2221 gacatcctgg tcgagggtcc gaagacgacc gcggtgaaca ccaccggaga gaacccgaac
     2281 cccacgcctc cggtggtcga gaccccgcag gagcagctca agcgggaccc gcagctgcag
     2341 cgggcgctgg atctgttgaa ggcgatgaag atcctggaca agacgcggcc gacgccgacg
     2401 actccgcagg cgcaagcccg ctaggcccac gggagcccac gagtgccggc gagagtgtgg
     2461 caggctctcg ccggcctcat cgtcctgctg ctgatcgcgg tcgtcggtct cgaccagtgg
     2521 caggcgcggc gtggagaggc gagcctgttc ggacaggggc tcctcggctc tgccccgtca
     2581 cggtcggtcg cgccggcccc gtccgtggtc acccggccga gccccccgct cgaggcggcc
     2641 ccggccccgc cgcccccggg agcgccccgg atcgcggtca tcgtcgacga cctgggcggg
     2701 cgccgagacg tgttcgacgt gctccgggag atccgtcggc cgctgacggt ggcggcgctc
     2761 cccgccctgc cgctctcggc gtcgatcgcg cgagacgcgt tccggtccgg gatggaggtg
     2821 ctgctggacg tccccatgga gccctaccgg tacccggagc tcgatccggg gccgggcatg
     2881 ctgctgacgt cgatggcccc cggcgagatc cagcacgcgg tggggacgca gctcgagacg
     2941 gtggcgtcgg cggtgggggt gatgaaccgg atgggctcgc ggctcaccga ggatcggacg
     3001 cggatgcggg cgatgctcga ggtgctggcg gcccggcgcc tgttcctggt ggacgcctac
     3061 acgtcgagcc agtcgctcgc gttcgacgag gcgcgcagcg cgggggtgcg cgcggcgcgc
     3121 cggcacatcc tggtggatca cacccgcggg gaagccggag accggctacg atgggacgag
     3181 gtggcagggt gggcggagcg gcggggcgag gtcatcgtgg tggcccacgg gcatccgctg
     3241 acggtgaagc tcttgaagga atacgt
//
LOCUS       DATMCV010000628         3187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_436082, whole genome shotgun sequence.
ACCESSION   DATMCV010000628 DATMCV010000000
VERSION     DATMCV010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436082"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2892
                     /locus_tag="VNU03_14485"
     CDS             <1..2892
                     /locus_tag="VNU03_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75725.1"
                     /translation="VRLFQELDASTRELTRSVGELQALGEVSQALGSTLDLQAVLATI
                     VGRAVQLSGSYSGIAYEFDEATQIFHARATHQISPEFLDVTQAAPLRLGEGAVGRAGV
                     IRQPVEVADVEAEAQLVAPQVRSLLAREGLRSLLAIPLAREDRLLGGLVIIRRERGGF
                     SPEVVALLQTFAAQSVLAIHNAGLFREIQRQKQYADALVETSPVAIATGDLRGVVVGW
                     NPGAERLFGYSAGEAIGHELVDLVATPDNREDVIADSRAALRGERLHAMARRARKDGT
                     LVDVEMSTMPLVVEGAEVGFIAIYHDVTELLRARREAETANEAKSAFLATMSHEIRTP
                     MNAVIGMSGLLLNTRLSEEQRDYAEIIRGSGDTLLTVINDILDFSKIEAGKLELESQP
                     FDLRECVEGALDLVATRAAEKGIDLAYLLGDGVPAAIVGDVTRLRQVLLNLLSNAVKF
                     TERGEVVLSVGARPPGRGSALHELTFSVRDTGIGIPADRLGRLFQSFSQVDASTTRRY
                     GGTGLGLAISQRLTEMMGGRIGVTSEEGVGSDFHFSIRAAATDVPVQIHRDLSGVQPS
                     LRGKRVLVVDDNATNRRIVTAHLEIWGMASRASESPLESLEWVRGGERFDLAILDMHM
                     PEMDGVALARAIRQAPAGATLPLVLFTSLGRREARADDEGFAAYLHKPIKPSQLFDAL
                     VSVLADQPIHVAASRSGSSALDPEMAGRHPLRILLAEDNVVNQKVALRLLAQMGYRAD
                     VAANGLEAIEAVERQTYDVVLMDVQMPELDGFEASREINRRWPGGRRPRIVAMTANAM
                     QGDRELCVAAGMDDYVAKPIRVEELVAALERSPVHTSLPVAGAPAGGADATAIDPATF
                     ERLVVTTGGPFVAELIDTFAEDARDLLVALRQTLARTDLDGFRRAAHSLKSTAESLGA
                     LPLAALAREVEAEARAGRLEGAAPRIDRLAEHYERAARALGDLRRDLSA"
     gene            2876..>3187
                     /locus_tag="VNU03_14490"
     CDS             2876..>3187
                     /locus_tag="VNU03_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75726.1"
                     /translation="MTSPPNGPAGDLLVVDDNRVNRLLLGRALEQLGHAVTFAENGRQ
                     ALEILGQRRVDLILLDIEMPEMDGYQVLAALAADPRLRDVPVVMMSSVEEVDSVARCI
                     EM"
BASE COUNT          437 a         1188 c         1141 g          421 t
ORIGIN      
        1 gtgcgcctgt tccaggagct ggacgcgagc acgcgggagc tgacgcgctc ggtgggcgag
       61 ctgcaggccc tcggcgaggt gagccaggcc ctcggctcca ccctcgacct ccaggcggtc
      121 ctcgcgacca tcgtcggccg cgcggtccag ctctccggca gctacagcgg gatcgcctac
      181 gagttcgacg aggccaccca gatcttccac gcgcgggcca cccaccagat cagcccggag
      241 ttcctggacg tgacgcaggc ggcgccgctc cggctcgggg agggcgcggt cgggcgcgcg
      301 ggcgtgatcc ggcagccggt cgaggtcgcc gacgtcgagg ccgaggccca gctggtcgcc
      361 ccccaggtcc ggagcctcct ggcccgggag ggcctgcgct cgctcctcgc gatccccctc
      421 gcccgggaag accggctcct cggcggcctg gtcatcatcc gccgcgagcg gggcggcttc
      481 tcgcccgagg tcgtcgcgct gctgcagacc ttcgcggccc agtcggtgct ggccatccac
      541 aacgcgggcc tcttccgcga gatccagcgc cagaagcagt acgcggacgc cctcgtggag
      601 acgagcccgg tggcgatcgc gacgggcgac ctgcggggcg tcgtggtggg ctggaacccc
      661 ggcgcggagc ggctgttcgg ctacagcgcg ggggaggcga tcggccacga gctcgtcgac
      721 ctggtggcga cgccggacaa ccgcgaggac gtcatcgccg actcccgggc cgcgctccgg
      781 ggcgagcggc tccacgcgat ggcccgtcgc gcgcggaagg acgggaccct ggtcgacgtc
      841 gagatgtcga cgatgcccct ggtggtggag ggagccgagg tggggttcat cgcgatctac
      901 cacgacgtca ccgagctgct gcgggcgcgg cgggaggcgg agaccgccaa cgaggccaag
      961 agcgcgttcc tggccaccat gagccacgag atccgcaccc cgatgaacgc ggtcatcggg
     1021 atgagcgggc tgctgctcaa cacccggctc tccgaagagc agcgcgacta cgcggagatc
     1081 atccgaggca gcggcgacac gctcctcacc gtcatcaacg acatcctcga cttctccaag
     1141 atagaggcgg gcaagctcga gctggagtcc cagcccttcg acctgcggga gtgcgtggag
     1201 ggggccctcg acctggtggc cacccgcgcg gccgagaagg ggatcgacct ggcctacctc
     1261 ctgggcgacg gcgtgcccgc cgcgatcgtg ggcgacgtga cgcgcctgcg ccaggtgctg
     1321 ctcaacctgc tctcgaacgc ggtcaagttc accgagcgcg gcgaggtcgt gctctcggtg
     1381 ggcgcgcgcc cgccgggccg cggctcggcg ctgcacgagc tgaccttctc ggtgcgcgac
     1441 accggcatcg gcattcccgc ggaccggctc ggccgcctct tccagtcgtt cagccaggtc
     1501 gacgcctcga cgacccgccg ctacggcggt accgggctcg gcctcgccat cagccagcgc
     1561 ctgaccgaga tgatgggcgg ccggatcggc gtgaccagcg aggagggcgt gggcagcgac
     1621 ttccacttct cgatccgggc cgccgccacc gacgtgccgg tccagatcca ccgcgacctg
     1681 agcggggtgc agcccagcct gcgcggcaag cgggtgctcg tggtcgacga caacgctacc
     1741 aaccgccgca tcgtgaccgc ccacctcgag atctggggca tggcgtcgcg ggcgtccgag
     1801 tcgccgctcg agtccctcga gtgggtccgg ggcggtgagc gcttcgacct cgcgatcctg
     1861 gacatgcaca tgccggagat ggacggcgtc gccctggccc gggcgatccg gcaggcgccg
     1921 gcgggggcga cgctccccct ggtgctcttc acctccctcg gccgccgcga ggcccgcgcg
     1981 gacgacgagg gcttcgccgc gtacctccac aagccgatca agccctccca gctcttcgac
     2041 gcgctggtct cggtcctggc cgatcagccg atccacgtgg ccgcgagccg gtccgggtcg
     2101 agcgcgctcg acccggagat ggccgggcgc cacccgctcc ggatcctcct cgccgaggac
     2161 aacgtggtca accagaaggt cgccctccgg ctcctggccc agatgggcta ccgggccgac
     2221 gtggccgcca acggcctcga ggccatcgag gcggtcgagc gccagaccta cgacgtcgtc
     2281 ctcatggacg tgcagatgcc ggagctcgac ggcttcgagg cctcccgcga gatcaaccgc
     2341 cgctggccgg gcggccgccg gccccgcatc gtggccatga ccgccaacgc catgcagggc
     2401 gaccgggagc tgtgcgtggc cgcgggcatg gacgactacg tggccaagcc gatccgggtc
     2461 gaggagctgg tcgccgcgct cgagcgctcc ccggtccaca cgagcctccc cgtcgcgggc
     2521 gctccggccg ggggcgcgga cgcgaccgcc atcgatcccg cgaccttcga gcgcctcgtg
     2581 gtcacgaccg gcggcccgtt cgtggcggag ctgatcgaca ccttcgccga ggacgcccgc
     2641 gacctgctgg tcgccctgcg acagaccctc gcccggaccg acctggacgg ctttcgccgg
     2701 gccgcccact ccctcaagtc caccgccgag agcctcggcg cccttccgct ggccgcgctc
     2761 gcgcgcgagg tcgaggcgga ggcgcgggcg ggccgcctcg agggagccgc cccgcggatc
     2821 gatcggctgg ccgagcacta cgagcgcgcc gcgcgcgcgc tgggagacct gcgacgtgac
     2881 ctctccgcct aacgggccgg ccggcgatct cctcgtcgtc gacgacaacc gggtcaaccg
     2941 cctgctgctc gggcgcgcgc tcgagcagct cggtcacgcg gtgacgttcg cggagaacgg
     3001 ccggcaggcc ctcgagatcc tcggccagcg ccgcgtcgac ctgatcctcc tcgacatcga
     3061 gatgccggag atggacggct accaggtgct cgccgcgctg gccgccgacc cgcgcctccg
     3121 cgacgtcccg gtggtcatga tgtcctcggt cgaggaggtg gacagcgtcg cgcggtgcat
     3181 cgagatg
//
LOCUS       DATMCV010000629         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_436151, whole genome shotgun sequence.
ACCESSION   DATMCV010000629 DATMCV010000000
VERSION     DATMCV010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436151"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VNU03_14495"
     CDS             complement(<1..260)
                     /locus_tag="VNU03_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75727.1"
                     /translation="MTRRLTVRLVLAAWTTALIVWLFAACAAGRPTSLTRLREGYEVL
                     TAHCEARAPESCTIVHVTGDPHDEEAWGFRARPAANALWKEP"
     gene            488..823
                     /locus_tag="VNU03_14500"
     CDS             488..823
                     /locus_tag="VNU03_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75728.1"
                     /translation="MLVADDHADLVELLQTVLGGEGYPVDAVLGGQGALERLEQTVYD
                     LVLCDLAMPEVDGVAVCRAGAQLASPRRVRGYLHAGDILQTTHAVVLTKPVGLDTLRD
                     LTERWLGGR"
     gene            828..1265
                     /locus_tag="VNU03_14505"
     CDS             828..1265
                     /locus_tag="VNU03_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75729.1"
                     /translation="MHVIGRSGLVALLSAAWLVIPAHAQDHVRAPVPGDVDGWVTQAT
                     IHRTICQPGYTATVRPPRAVTDAAKRRLLNGHPGSRTDYELDHLIPLGLGGHPTSSNN
                     LWFQHWTEAAIKDRDELRLHRELCAGRVTLEAAQREMRAAWRP"
     gene            1345..1710
                     /locus_tag="VNU03_14510"
     CDS             1345..1710
                     /locus_tag="VNU03_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75730.1"
                     /translation="MPRILIVEDDPHLRDVVQLILAGPGNEIDSVADGQAALERLAQH
                     AYDLVVCDLHMPGVDGRAVYLAIERQSPPRPAVVFVTGSHDTGAYKAWLRTVHAAVVG
                     KPFDITVLRETVRRLLSGP"
BASE COUNT          319 a          666 c          748 g          312 t
ORIGIN      
        1 tccggttcct tccacagcgc gttggccgcc ggccgcgccc ggaaccccca cgcctcttca
       61 tcatgtgggt ccccggtcac gtgcacgatc gtacaggact cgggtgcccg cgcctcgcaa
      121 tgcgcggtga ggacctcata accctcgcgc aaccgggtca gactcgtcgg ccgccccgca
      181 gcacaggccg cgaacagcca cacgatcagc gccgtcgtcc atgccgctag tacgagccgc
      241 accgtcagcc tccgggtcat ggccgaagga tagcaggcgg cggcgcggcg cggggcgacg
      301 caacttcgcc tcgcttctag acatggcgcc cgttttcccg ggcgtgtccg ctggccgcgc
      361 cgcgtcacgg agggccgatg gtcccgagga tcgacctggg atgtgggcag gggttggatt
      421 ggaccgaaaa attccttccg gccatgcttg agaaggggcc accggagaca gggagctatg
      481 gtgaaggatc ctcgtcgccg acgatcatgc ggatctggtg gaactgctcc agacggtgct
      541 cgggggcgaa gggtacccgg tcgacgccgt gctgggtggg cagggcgcgc tcgagcgcct
      601 ggagcaaact gtctatgacc tggtcctctg cgacctggcg atgccggagg tggacggggt
      661 cgcggtctgt cgcgcggggg cgcagctggc gtccccgcgg cgggtacgcg gatacctcca
      721 cgcaggggat atcctgcaaa caacgcacgc ggtggtgttg accaagcccg tgggcctcga
      781 cacgctccgc gacctgaccg agcgttggct cgggggtcgg tagcctcatg cacgtcatcg
      841 ggcgcagcgg gctagtcgcc ctgctgagcg cggcgtggct cgtgatcccg gcccacgcgc
      901 aggaccacgt gcgagccccg gtccccgggg acgtggatgg gtgggtgacc caggcgacga
      961 tccaccgcac gatctgccag cccgggtaca cggcgacggt gcgcccgccg cgggccgtca
     1021 ccgatgccgc caagcggcgc ctcctgaatg gacacccggg gtcgcggacc gactacgagc
     1081 tcgatcacct gattccgctc gggctgggtg ggcacccgac gtcgtccaac aatctctggt
     1141 tccagcactg gacggaggcc gcgatcaagg accgggacga gctccggctg catcgggagc
     1201 tctgcgccgg ccgggtgacg cttgaggcgg cgcagcgcga gatgcgggcg gcgtggcgcc
     1261 catgaggacg gcacgcgggg agtgttcgct ggccggaccg cgttcaaggg agagcagcgt
     1321 gagagccggt aggtagacta aaccatgccc cggatcctga tcgtcgagga cgacccgcac
     1381 ctgcgcgacg tcgtccagct gatcctcgcc ggaccgggga acgagattga cagcgtcgcc
     1441 gacggccagg ccgcccttga gaggctcgcg caacacgcgt acgacctcgt cgtctgtgat
     1501 ctgcacatgc cgggtgtgga cgggcgggcg gtctaccttg cgatcgagcg gcagtctccg
     1561 ccccgaccgg ccgtcgtgtt cgtcacgggt tcgcacgaca cgggagcgta caaggcctgg
     1621 ctcagaaccg tgcacgcggc ggtggtgggc aaaccgttcg acatcacggt gctacgagag
     1681 acggtgcgcc gactgctcag cggaccctga agatgtctcc gtcgatggcg aggtggacgg
     1741 ccaccggctc atcgtgccgg cgcggcgtct ggccgacgag cggttcgcag cctgggccgc
     1801 ggtgctcgcg cgcggctacg ggggcttggt gagcaaggac gactcctcgc cctccgtcgg
     1861 cgggcgcacc ctgaagtggc tcaaggtcag actgtcgagg cgtcgttctc cgtattccgg
     1921 gggaccagag cggcctggtt gttcgtgact gggagaaagg ggctcggaag gtcggcctga
     1981 agctccgggt gatcgagctg aagggggacg ggacggagga tttaccagcg gccttccaga
     2041 gcgct
//
LOCUS       DATMCV010000630         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_101111, whole genome shotgun sequence.
ACCESSION   DATMCV010000630 DATMCV010000000
VERSION     DATMCV010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101111"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VNU03_14515"
     CDS             <1..656
                     /locus_tag="VNU03_14515"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75731.1"
                     /translation="SIPIVFIAHADPVGTGHVASLARPGGNITGLAILQTEIGAKGLE
                     ILRSVIPTVTRIAVLWHPGTPSAAPGLKALEEPARRLRLELQPVGARSPAELEGAFAA
                     MARARAQGVLVLATPIFFGERQRLGELAIARRLPTMFQIRDYAEAGGLMSYGADLADL
                     YRRAAIPVDKILKGARPGDIPIEQPTKFELIINLKTVKALGLTIPPSLLQRADQVIE"
     gene            749..1090
                     /locus_tag="VNU03_14520"
     CDS             749..1090
                     /locus_tag="VNU03_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75732.1"
                     /translation="MKTFRLYTGSDDQSHVEPIDLARTPGWTKGLATTQIAFRESPVG
                     NFIDWHPAPRRQFVIILSGQLEIGLGDGSKHLFGPGDARLVEDVTGKGHTTRVHGSQP
                     CLTATIPLAEQ"
     gene            1093..2073
                     /locus_tag="VNU03_14525"
     CDS             1093..2073
                     /locus_tag="VNU03_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HWP75733.1"
                     /translation="MATKILFAPKQSDAVLEIARSLTPAGFELVIADIGTPEFYAAAA
                     QAEYYLGLARQMGGEFFRSAPNMKLVQLLSAGYDRVDIEAARKAKVPVSNNGGANAIA
                     VAEHTIMLILAVLKRVVRFHNDVVAGKWRVGGFDDQRVYELSRKTLGIVGLGNIGKKV
                     ARRAAAFDMAVQYYDIARLTEAEEDALGVRFVLLPELLRTSDVVSLHVPLDDSTRHLL
                     GAREFAQMKPGAILINTCRGPVVDEAALHAALKSGQVAGAGLDVLVEEPPATNHALFA
                     LPNVTLTPHSAGPTWENWTARFRNGFDNIQRVAAGRAPKWVIPELQGPQP"
BASE COUNT          352 a          788 c          688 g          310 t
ORIGIN      
        1 cgtcgatccc catcgtcttc atcgcccatg cggatccggt cgggaccggc cacgtcgcga
       61 gcctcgccag gcccggcgga aacatcaccg gactggcaat actgcagacc gagatcggcg
      121 ccaaggggct cgagatcctg cggtctgtca ttcccacggt cacccggatc gccgttctct
      181 ggcatcctgg cactccgtcc gccgccccgg gactcaaggc gctggaagag ccggcgcgga
      241 ggctgcggtt agagttgcag cccgtcggag cgcggagccc ggcagagttg gagggcgcct
      301 tcgcggccat ggcccgggcg cgcgcgcagg gcgttctcgt acttgccacc ccgatcttct
      361 tcggtgagag acagcgcctc ggcgaattgg ccattgcgcg ccggctaccg acgatgttcc
      421 agataaggga ttacgcggag gccggcggtc tgatgagcta cggcgccgat cttgccgacc
      481 tctaccggcg cgccgccatc ccggtcgaca agatcctcaa gggcgccagg cccggcgata
      541 ttcccatcga gcagcccacg aagttcgagc tgatcatcaa cctgaagacc gtcaaggccc
      601 tcggcctgac gatcccgccg tcgctcctcc agcgggcgga ccaggtgatc gaatagagga
      661 aaccagagtc gacgcgccgc gccatcacgc gcaccggatg atataatccg cgcgcccatc
      721 ccacgaggcg gcaccgagag aggcgatcat gaagacattc cggctctaca ccggcagcga
      781 cgaccagtcc cacgtcgaac cgatcgacct cgcgcggacc ccgggctgga ccaagggact
      841 cgcgaccacc cagatcgcgt tccgcgagag cccggtcggc aacttcatcg actggcatcc
      901 cgcgccccgg cgccagttcg tgatcatcct gtcggggcag ctcgagatcg gtctgggcga
      961 cggctcgaag cacctgttcg gtccgggcga cgcccgcctg gtcgaggacg tcaccggcaa
     1021 gggccacacc acccgcgtgc acggcagcca gccgtgcctc accgccacca tccctctcgc
     1081 ggagcaatag ccatggccac caagatcctc ttcgcgccca agcagtccga cgcggtgctc
     1141 gagatcgccc gctcgctcac ccccgcgggc ttcgagctgg tcatcgccga catcggcacc
     1201 ccggagttct acgccgccgc ggcccaggcc gagtactacc tgggcctcgc ccgccagatg
     1261 ggcggcgagt tcttccgctc cgccccgaac atgaagctgg tgcagctgct ctccgcgggc
     1321 tacgaccggg tcgacatcga ggcggcccgc aaggcgaagg tgccggtgtc caacaacggc
     1381 ggggccaatg cgatcgcggt ggccgagcac accatcatgc tgatcctggc cgtgctcaag
     1441 cgggtggtcc gctttcacaa cgacgtggtg gccggcaagt ggcgggtcgg cggcttcgac
     1501 gaccagaggg tctacgagct gtcgcgcaag accctcggca tcgtgggcct cggtaacatc
     1561 ggcaagaagg tggcccggcg cgcggccgcg ttcgacatgg cggtgcagta ctacgacatc
     1621 gcccgtctca ccgaggccga ggaggacgcc ctcggggtcc gcttcgtgct gctccccgag
     1681 ctgctgcgca cctccgacgt ggtgagcctg cacgtgccgc tcgacgactc cacccgccac
     1741 ctgctggggg cccgcgaatt cgcccagatg aagccgggcg cgatcctgat caacacttgc
     1801 cggggcccgg tggtggacga ggccgcgctc cacgccgcgc tcaagagcgg ccaggtcgcg
     1861 ggcgcggggc tcgacgtgct ggtcgaggag ccgcccgcca ccaaccacgc gctcttcgcg
     1921 ctgcccaacg tgaccctcac cccgcattcc gcggggccca cctgggagaa ctggaccgcg
     1981 cgcttccgca acggcttcga caacatccag cgggtggccg ccggccgggc ccccaagtgg
     2041 gtgatccccg agctgcaagg cccgcagccg tgaagatcct cttcgccccg aacctgatcg
     2101 tctccggcct cggcgaggtc gaccgcgccg gcatcctc
//
LOCUS       DATMCV010000631         2879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_970258, whole genome shotgun sequence.
ACCESSION   DATMCV010000631 DATMCV010000000
VERSION     DATMCV010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970258"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VNU03_14530"
     CDS             complement(<1..1133)
                     /locus_tag="VNU03_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP75734.1"
                     /translation="MAHVTLASMFWNRVETDGDRPAQQWKEGGAWRTRPWREVGEIVR
                     ELATGLLALGRRRDEAIGILSASRPEWVQADFAAFSAGCRTIPIYPSYPPDLIQYIVN
                     DAGVKTLFVEDPAQLAKVLEVQGKMEGLEQIVVMQGHQGESSARVMTWDGLRRLGRDN
                     LERLRGELASRVAAVGPEDIATIVYTSGTTGPPKGVVQTHGNHLGTLASAAKMSGIQD
                     GDTHLLFLPLAHSFARLESFIGVHRGLTTAFAESIDKLRENLPEVKPHFICSVPRVFE
                     KVYAGVLAKAEAGSPVKRKIFNWAVGVGREVSKLQQARRPVPAVLGFKHRIAHALVFG
                     KLHEALGGRLRFAVSGGAPLSKEIAEFFHAAGILILEGWGLTET"
     gene            complement(1144..2649)
                     /locus_tag="VNU03_14535"
     CDS             complement(1144..2649)
                     /locus_tag="VNU03_14535"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWP75735.1"
                     /translation="MSLTRLADESLDKYGEYVALAFEGRRYTNLEQARASARLAHALR
                     RLGVGPGDRVLVMLPNCPEVIQSYGAILRCGGIIVPVIFLLGEREVAHIVADSEAKVV
                     ITSADLLGKVEAQIGVLPTLHHVLLVDGGGDGTTRSLAAEAAGEPDTFPAVDRGSDDL
                     AVILYTSGTTGLPKGVALSHDNLESNARSAASLNELGRDDWAVAVLPLSHSYGLTVMN
                     AGHLLGSRAALLRWFHPEAVLQTIQEFRAVSMSAVPTMLLYLLNYPEADRFDTRSMRA
                     WGSGAAPLPTEIVEPFERKFGGRILEGYGLTEASPVVSAHGLSGPRKVGSVGRAIPGV
                     EVSIQDDSDRALPAGEVGEVCVRGRSVMTGYYRNPEETARTVRAGWLHTGDMGRLDAD
                     GFLFIVERKKDLIIRGGFNIYPREVEEVLYAHPKVAEAAVVGMKDELMGEGVLAFVVL
                     RSGERASAEEIGAFCEGRLARFKCPKEIRFVDSLPKSPIGKILRKELRTQA"
BASE COUNT          449 a         1023 c          980 g          427 t
ORIGIN      
        1 gtctcggtca ggccccagcc ctcgaggatc aagatgcccg ccgcgtggaa gaactcggcg
       61 atctccttgg acagcggggc gccgcccgag accgcgaagc gcagacgccc gccgagggcc
      121 tcgtgcagct tgccgaacac cagggcgtgg gcgatgcggt gcttgaagcc gagcaccgcc
      181 ggaaccggac ggcgcgcctg ctggagcttc gacacctccc gacccacccc gaccgcccag
      241 ttgaagatct tgcgcttgac cggcgagccg gcctccgcct tggcgaggac gcccgcgtac
      301 accttctcga acacgcgggg cacgctgcag atgaagtgcg gcttcacctc gggcaggttc
      361 tcgcggagct tgtcgatgct ctcggcgaag gcggtggtga gcccgcggtg cacgccgatg
      421 aacgactcga gccgtgcgaa ggagtgcgcg agcggcagga acagcaggtg ggtatcgccg
      481 tcctggatcc ccgacatctt ggccgcggac gcgagcgtgc ccaggtggtt gccgtgggtc
      541 tggaccacgc ccttgggcgg gccggtggtg cccgaggtgt acacgatggt ggcgatgtcc
      601 tccggcccga ccgcggccac ccggctcgcc agctcgccgc ggagccgctc gaggttgtcc
      661 cgtccgaggc gccgcagccc gtcccaggtc atgacccggg ccgaggactc gccctggtga
      721 ccctgcatca ccacgatctg ctcgagccct tccatcttcc cctgcacctc gaggaccttg
      781 gcgagctgcg ccgggtcctc gacgaacagg gtcttcaccc ccgcgtcgtt gacgatgtac
      841 tggatgaggt ccggggggta gctcggatag atcggaatgg tgcggcagcc cgcggagaag
      901 gccgcgaagt cggcctgcac ccactccggt cgactcgcgg acaggatgcc gatcgcctcg
      961 tccctgcggc gcccgagggc gagcaggccg gtggccagct cgcgcacgat ctccccgacc
     1021 tcgcgccagg gacgggtgcg ccacgcgccg ccctccttcc attgctgcgc cggccggtcg
     1081 ccgtcggttt cgacccgatt ccagaacatg ctcgccagcg tcacgtgggc catggttccc
     1141 tcctcacgcc tgtgtgcgga gctctttgcg caggatcttg ccgatcgggc tcttgggcag
     1201 cgagtccacg aagcggatct ccttcggaca cttgaagcgg gccagacggc cctcgcagaa
     1261 cgcgccgatc tcctcggcgg acgcccgctc gccgctccgg agcacgacga acgcgagcac
     1321 gccctccccc atcagctcat ccttcatgcc caccacagcg gcctcggcca ccttggggtg
     1381 ggcgtagagc acctcttcga cctcgcgcgg gtagatgttg aacccaccgc ggatgatcag
     1441 gtccttcttg cgctcgacga taaagaggaa gccgtccgcg tcgagccggc ccatgtcccc
     1501 ggtgtggagc catccagccc gaaccgtccg ggccgtctcc tccgggttgc ggtagtagcc
     1561 ggtcatgacg ctccgcccgc gaacgcagac ctcgcccacc tcgcccgccg ggagcgcgcg
     1621 gtcggaatcg tcctggatcg agacctcgac cccgggaatc gcgcgcccca ccgagcccac
     1681 cttgcgcggg ccgctcaaac cgtgggccga caccaccggc gaggcctcgg tcaggccgta
     1741 gccctcgagg atccggccgc cgaacttccg ctcgaagggc tccacgatct cggtgggcag
     1801 cggggccgcg cccgagcccc acgcccgcat cgagcgggtg tcgaagcggt cggcctccgg
     1861 gtagttcagc aggtagagca gcatggtggg caccgccgac atcgagaccg cccggaactc
     1921 ctggatggtc tgcagcaccg cctccgggtg gaaccagcgg agcagcgccg cccgcgagcc
     1981 caggaggtgg cccgcgttca tcacggtgag gccataggag tgcgagagcg gcaggaccgc
     2041 caccgcccag tcgtcccgcc cgagctcgtt cagcgaggcg gccgagcgcg cgttggactc
     2101 gaggttgtcg tgggagaggg ccacgccctt ggggaggccg gtggtccccg aggtgtagag
     2161 gatcaccgcg aggtcgtcgg agccccggtc cacggcgggg aaagtgtccg gctctcccgc
     2221 ggcctccgcg gccagcgagc gcgtcgtgcc gtcgccgcct ccgtcgacca gcaggacgtg
     2281 gtgcagcgtg ggcaggacgc cgatctgcgc ctcgaccttc ccgagcaggt cggcggacgt
     2341 gatcaccacc ttcgcctcgg agtcggccac gatgtgggcg acctcccgct cgcccagcag
     2401 gaagatcacc gggacgatga tgccgccgca cctgaggatg gccccgtagc tctggatcac
     2461 ctcggggcaa ttcgggagca tcacgagcac gcggtccccg ggcccgaccc ccagacgccg
     2521 cagggcgtgg gccagccggg cggaagcccg cgcctgctcc agattggtgt accggcgtcc
     2581 ctcgaatgcg agcgcgacgt actcgccgta cttgtcgagg ctctcgtccg cgaggcgagt
     2641 gagggacatg gctcgggtca ccagttcgtg atgagtttca agaggttacc cgtatagcac
     2701 gggcgccatg acgcggcgca ataaaacgct tgacgctcat agcgcaccgc gttatgctcg
     2761 gccgtgcggt gacagaaacg ccaccaagga gtgaaccgat gaccaattcc ctgcaggtga
     2821 acgagctgat gggcgagctg accggacgca ccgtcgaggt attttcgctg tgggccgac
//
LOCUS       DATMCV010000632         4417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_970297, whole genome shotgun sequence.
ACCESSION   DATMCV010000632 DATMCV010000000
VERSION     DATMCV010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970297"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VNU03_14540"
     CDS             complement(<1..920)
                     /locus_tag="VNU03_14540"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75736.1"
                     /translation="MMGRQLARIAVRHLARHPWQLGLAVLGIALGVAVAVSIDLANES
                     ARRAFSMATVAVTGRATHQVVGGPSGLPDDFYRVLKVELGARLAAPVLSGDVAAADRP
                     GRTFTVLGVDPFAEAPFRPYLEAEGVRETGTTESGSSAPGAAAGERRDPPPRDRLSTV
                     AALVTRPGAILVARPTARELGLGVGDRLAVRAAGIRRELTVAGLLDPGDDASARALDG
                     LLVTDISSAQEIFGAAGRLGRVDLIVTDDEAGRALLARITRALPPGAELVAAGARAGT
                     TAQMIRAFQWNLTALSLLALVVGMFLIYQT"
     gene            complement(917..1645)
                     /locus_tag="VNU03_14545"
     CDS             complement(917..1645)
                     /locus_tag="VNU03_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75737.1"
                     /translation="MPGPAVELLHVGKTYREGDSERAVLSDVSLAIEPGEIVVLVGRS
                     GSGKSTLLNLIAGIDRPTAGRVVVDGTDLTALDEDARTRFRRRRIGFVFQFFNLIPLL
                     SVEENLLLPLELNGKADAAGVARARGLLERVGLGDRGASLPDRLSGGEQQRVAIARAL
                     VHDPALILADEPTGTLDAETAAAVLALLDGLAREAGKTVVMVTHSREVVGVADRIFDV
                     QRGRLVEQPIAPGAPGRGAPAASG"
     gene            complement(1690..1893)
                     /locus_tag="VNU03_14550"
     CDS             complement(1690..1893)
                     /locus_tag="VNU03_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75738.1"
                     /translation="MRFWVKEGLRIGAVIAIAAGLLWLLMERDVGRAALLRSTVWALV
                     VWAVCGVVWIGLQYGQWHEVENP"
     gene            complement(1897..2928)
                     /locus_tag="VNU03_14555"
     CDS             complement(1897..2928)
                     /locus_tag="VNU03_14555"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWP75739.1"
                     /translation="MPGILDHVEWVLFGWVLANQSGVPVPVVPGLVAAGALAANGRTS
                     IVEIFTVAVVATLCADLGWYGLGRWRGAGVLSLLGGLSPRASALVRRAHDGFLAHAGK
                     FQLTARFLPELNPVAAGLAGATRVRVARFVGYGAFSALVWAGIWTGLGYLLGDAIVDF
                     ATRFGIRLTGFALGALLLCLVIRRARRHRMLRMLQRARITADDLKARLDRGERITILD
                     LRGPAEVACHPYMIPGALGMESGASLRRLRELPRDAMVVVYGRRFKRARGERATPHTN
                     VILGLYRVGFGRVRPLAGGLRAWRRRGYPVMAPPAVLPNGLGNGRPAPVEREASPVPR
                     ANELATRGA"
     gene            complement(3040..3291)
                     /locus_tag="VNU03_14560"
     CDS             complement(3040..3291)
                     /locus_tag="VNU03_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75740.1"
                     /translation="MTEGQGIDSSLLAEIGSRLERLSEARHRLARHHRILVRAATELR
                     LGRSAEGVLAQIREQCPELLALAPAPAPVRPAGRIAISA"
     gene            complement(3291..4349)
                     /locus_tag="VNU03_14565"
     CDS             complement(3291..4349)
                     /locus_tag="VNU03_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP75741.1"
                     /translation="MITTLPTRPLGRSGLSITRVGFGSWAIGGGGWSFGWGPQDDAAS
                     LATMRHAVERGINWIDTAAVYGLGHSEELVGRLLRELPPAQRPLVFTKCGMIWDEQDP
                     MAAPRQVLKPDSIRRECEASLRRLGVERIDLYQFHWPDETGTFLEDSWAAMVKLVEEG
                     KVRAAGVSNFDSRLLDRCEAIRHVDSLQPPFSLINRAAGKREIPWCARHETGVIVYSP
                     MQSGLLTDAFTAERVTALPSDDWRRRAPDFRLPRLRRNLALRDALRPIALRHGTSVSS
                     IAIAWTLAWPGVTGAIVGARTPEQVDGWIGAAAIALTPEDLEEIGAAIGRTRAGAGPV
                     RVEEPSRRKVARGHGGRA"
BASE COUNT          625 a         1693 c         1557 g          542 t
ORIGIN      
        1 atcgtctggt agatgagaaa catgccgacg acgagggcga ggagcgacag cgcggtcagg
       61 ttccactgaa aagcgcggat catctgggcg gtggtacccg cccgcgcgcc cgcggcgacc
      121 agctccgccc cgggcggcag ggcccgcgtg atgcgggcga ggagcgcccg ccccgcctcg
      181 tcgtcggtga cgatgaggtc gacgcggccg agccgtcccg ccgctccgaa gatctcctgg
      241 gcgctcgaga tgtcggtgac gagcaggccg tcgagggcgc gagcgctcgc gtcatcgccc
      301 gggtcgagca gccccgccac cgtcagctcg cgacggattc ccgccgcgcg gaccgcgagc
      361 cggtcgccca ccccgaggcc cagctcacgc gcggtcggcc gcgccaccag gatcgcgccc
      421 ggacgcgtga cgagcgcggc caccgtggag aggcggtcgc gcggcggcgg gtcgcggcgc
      481 tcgccggccg cggcgccggg cgcgctcgat ccgctctcgg tcgtcccagt ttcccggaca
      541 ccctcggcct ccaggtacgg ccggaacggg gcctccgcga acggatcgac gccgagcacg
      601 gtgaaggtcc ggcccggacg atcggccgct gccacgtcgc cggacagcac cggcgcggcg
      661 aggcgcgccc ccagctccac cttcagcacc cgatagaagt cgtcgggcag gccggacggg
      721 ccgccgacga cctgatgggt cgcgcgaccg gtgaccgcca cggtggccat cgagaaggcc
      781 cgccgcgcgc tctcgttggc gaggtcgatc gagaccgcga ccgccacacc gagcgcgatg
      841 ccgagcaccg cgaggccgag ctgccacgga tgccgcgcca gatgacggac ggcgatccgg
      901 gcgagctggc gccccatcat ccgctcgcgg ccggcgcccc gcggcccgga gcgccgggcg
      961 cgatcggctg ctcgacgagc cggccgcgct gcacgtcgaa gatgcggtcg gccaccccga
     1021 cgacctcgcg gctgtgcgtc accatgacca ccgtcttgcc cgcctcccgc gcgaggccgt
     1081 cgagcagcgc cagcaccgcc gccgcggtct cggcgtccag cgtgccggtg ggctcgtcgg
     1141 ccaggatcag cgcggggtcg tggacgagcg cccgcgcgat ggccacccgc tgctgctcgc
     1201 cgccggacag ccggtccggc aggctcgcgc cccggtctcc gagcccgacg cgctcgagca
     1261 gcccgcgcgc ccgcgccacc cccgccgcgt cggccttgcc gttcagctcg agcggcagga
     1321 gcaggttctc ctcgacggag aggaggggga tcagattgaa gaactggaag acgaagccga
     1381 tgcggcggcg gcggaatcga gtgcgcgcgt cctcgtcgag tgcggtgagg tcggtgccgt
     1441 cgaccaccac ccgaccggcg gtcgggcggt cgatgccggc gatgaggttg agcagcgtcg
     1501 acttccccga gccgctccgc ccgacgagga ccacgatctc cccgggctcg atcgccaggg
     1561 agacgtcgct cagcacggcc cgctcggaat caccctcgcg gtaggtcttg ccgacgtgga
     1621 ggagctcgac cgcgggtccg ggcatgcccc gccattgtac aggcccggcg cccggctacg
     1681 accctcgcgt caggggttct ccacctcgtg ccactggccg tactgcaggc cgatccacac
     1741 caccccgcac accgcccaca ccacgagcgc ccacaccgtg ctccggagca gcgcggcccg
     1801 gccgacgtcg cgctccatca gaagccagag cagcccggcc gcgatggcga tgaccgcgcc
     1861 gatgcggagg ccttccttga cccagaagcg cacgcctcag gctcctctcg tcgccagctc
     1921 gtttgcgcgg ggaacggggc tcgcttcccg ctcgacgggc gccgggcggc cgttgccgag
     1981 cccgttgggg agcacggcgg ggggcgccat caccggatag ccacgccggc gccaggcgcg
     2041 caggccgccg gcgagaggtc gcaccctccc gaacccgacg cggtacagtc ccaggatcac
     2101 gttcgtgtgc ggggtcgccc gctcgccccg cgcccgtttg aagcggcgac cgtacaccac
     2161 caccatggcg tcgcgaggaa gctcgcgcag ccgccgcaat gacgcgcccg attccatccc
     2221 gagcgcgccc ggaatcatgt acggatgaca cgccacctcg gcggggccgc ggaggtcgag
     2281 gatggtgatc cgctcgcccc ggtcgagccg cgccttcagg tcgtcggcgg tgatccgggc
     2341 tcgctgcagc atgcggagca tgcgatgccg gcgcgcgcgc cggatgacca ggcagaggag
     2401 cagggcgccg agcgcgaagc cggtcagccg gatcccgaag cgcgtcgcga aatccacgat
     2461 cgcgtcgccc agcagatacc cgagcccggt ccagatgccg gcccacacga gggcgctgaa
     2521 cgctccgtag cccacgaagc gcgcgaccct cacccgggtc gccccggcga ggccggccgc
     2581 caccggattc agctccggca ggaagcgcgc ggtgagctgg aacttgcccg cgtgagcgag
     2641 gaagccgtcg tgggcccgcc ggacgagggc gctggcccgg ggtgagagcc cgcccagcag
     2701 tgagagcacg cccgccccgc gccaccgacc caggccgtac cagccgaggt cggcgcagag
     2761 ggtggccacc acggcgacgg tgaagatctc gacgatgctc gtccggccgt tcgcggccag
     2821 cgcgcccgcg gccacgagcc cgggaacgac cggcaccggc accccgctct gattggccag
     2881 cacccagccg aacagcaccc actcgacgtg atcgaggatc cccggcatct ctcccacccc
     2941 ctttgtcgct cttgagatgc cggcgactat gccctcgtgc cgggccccgt ccccatcccg
     3001 caagaacgtg acaccaccgc cgagcggcgg gcgggcgctt caggcggaga ttgcgatccg
     3061 gcccgctggt cgaaccggcg ccggagccgg cgccagggcc agcagctcgg gacactgctc
     3121 ccggatctgg gccagcaccc cctccgcgct tctccccagc cgcagctccg tcgcggcccg
     3181 gaccaggatc cgatggtgcc gagccagccg gtgccgcgcc tccgacagtc gctcgaggcg
     3241 gctgccgatc tccgcgagca ggctgctgtc gatcccttgt ccctcggtca tcacgccctc
     3301 cctccgtgtc cgcgcgcgac ctttcgtcgg gacggctcct ccacccgcac cggccccgcc
     3361 cccgccctcg tgcgtccgat cgcggccccg atctcctcga ggtcctccgg ggtgagcgcg
     3421 atcgcggcgg cgccgatcca gccgtccacc tgctccggcg tacgcgcccc gacgatcgct
     3481 ccggtcactc ccggccacgc gagcgtccac gcgatggcga tcgacgacac ggacgtgccg
     3541 tgccgcagcg cgatcgggcg cagcgcgtcc cgcagggcca ggttccgccg gagccggggc
     3601 aggcgaaagt cgggcgcgcg gcggcgccag tcgtcggagg gcagcgcggt gacgcgctcg
     3661 gcggtgaagg cgtcggtcag caggccggat tgcattgggc tgtagacgat gacgccggtc
     3721 tcgtggcgcg cgcaccaggg gatctctcgc tttccggccg cgcggttgat cagcgagaag
     3781 gggggctgca gcgagtcgac gtggcggatc gcctcgcacc ggtcgagcag cctcgagtcg
     3841 aaattcgaga cccccgccgc gcggaccttc ccttcctcca cgagcttgac catcgcggcc
     3901 cacgagtcct cgaggaacgt gccggtctcg tccggccagt ggaactggta caggtcgatg
     3961 cgctcgaccc cgagccggcg cagcgacgcc tcacattcgc gccggatcga gtccggcttc
     4021 agcacctggc gcggggccgc catcggatcc tgctcgtccc agatcattcc gcacttggtg
     4081 aagaccagcg ggcgctgcgc gggcggcagc tcgcggagca gccgccccac cagctcctcg
     4141 gagtgcccga gcccgtagac cgcggcggtg tcgatccagt tgatgccccg ctcgaccgcg
     4201 tggcgcatgg tggccagcga cgccgcgtcg tcctgcgggc cccagccgaa cgaccagccg
     4261 ccgccgccga tcgcccacga gccgaaccca acccgggtga tggacagtcc gctgcggccc
     4321 agcggacgag tcggtagtgt cgtgatcacg cgtttctcct ttcgtcgtcg gtcatcggta
     4381 tgcgcggggg tatgcgcggg ggtatgcgcg ggggtat
//
LOCUS       DATMCV010000633         2052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_501247, whole genome shotgun sequence.
ACCESSION   DATMCV010000633 DATMCV010000000
VERSION     DATMCV010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501247"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2052)
                     /locus_tag="VNU03_14570"
     CDS             complement(<1..>2052)
                     /locus_tag="VNU03_14570"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75742.1"
                     /translation="EEFLTSLIESVPAIRVLLVLTYRPGYVNPFGERSYFTRVVPAGL
                     STGESARIAEAILGIDLFPAELQSLVAAKAEGNPFYTEELVRSLEESGALHRTEGRLV
                     LTRPVSQIAIPGTVQDVIAARIDRLAEAPKLTLQLASVIGREFGRRLVGRVSQIGEGT
                     DGALRELSALELILERRRLPELAYAFKHALTQDVAYASLLVQRRGEIHRLVGTAIEEL
                     YADRLPELYEVLAHHFSHTDDAGRALTYLMRAAEKATRAFGLRQAIDLYGEALAAAAR
                     LEDRAPATTVMAIHAARADLFFGVGDFVRSREAAETVVELARRVQDRPAEANALIQCA
                     SALQWAEDFPAALERAEEGIELAETAGAQRPLAGGLYVRGYVHAVSGRLDAAKDDVAR
                     ALAIGRAVGDPARQALALHILAMHSAWQGDYRGSLELAGEGVRLAREHRLVVPLLRCF
                     WNQGAALNDAGEYDAGLAALTEGLALAERIGDDAFIPRFQNTLGWLHMECGDLARGME
                     LSEQSYEVTGRSSRAGHGTGAERRAFIRNNEADAWMIRGDLASAADALAESSHIVLHP
                     PPSRWMTWRYAMHCHASQGQLALLQGDPDRARRLAEQSLELSVPTRSRKFEAWAWRIK
                     GESATARRAWEEADEALRRSFALAEAIGQPRQTWLSHVALGRLDAARGRRDDARARYR
                     GA"
BASE COUNT          277 a          783 c          717 g          275 t
ORIGIN      
        1 gcgccgcggt accgcgccct cgcgtcgtcc cgccgccccc gcgccgcgtc gagccggccg
       61 agcgcgacgt ggctcagcca ggtctggcgc ggctggccga tcgcctccgc gagagcgaac
      121 gagcgccgca gcgcctcgtc cgcctcctcc cacgcccgtc gcgcggtggc gctctcgccc
      181 ttgatgcgcc acgcccaagc ctcgaacttg cgcgagcgcg tcggcacgct cagctcgagg
      241 ctctgctcgg cgagccggcg cgcgcggtcg gggtcgccct gcagcagcgc gagctggccc
      301 tggctcgcgt gacagtgcat cgcgtagcgc caggtcatcc agcgggacgg cggcggatgc
      361 aggacgatgt ggctcgactc cgccagcgcg tcggccgcgc tcgcgaggtc gccgcggatc
      421 atccaggcgt cggcctcatt gttccggatg aaggcccggc gctcggctcc ggtcccgtgc
      481 ccggcccgcg acgagcggcc ggtcacctcg taggactgct cggacagctc catgcctcgc
      541 gcgagatcgc cgcactccat gtgcagccag ccgagcgtgt tctggaaccg tgggatgaag
      601 gcgtcgtcgc cgatccgctc cgccagggcc agcccttccg tcagggcggc gagcccggcg
      661 tcgtactcgc ccgcgtcgtt cagcgccgct ccctgattcc agaagcagcg caggagcggg
      721 acgaccagac gatgctcgcg ggcgaggcgg accccctcgc ccgccagctc caggctcccc
      781 cggtagtcgc cctgccaggc cgagtgcatc gcgagtatgt gcagcgccag ggcctgccgg
      841 gcgggatcgc cgaccgcccg cccgatggcc agcgcgcgcg ccacatcatc cttcgccgcg
      901 tcgaggcggc cgctgaccgc gtgcacgtac ccgcggacat acaggccgcc ggccagcggc
      961 cgctgggctc ccgccgtctc ggccagctcg atcccctcct ccgcgcgctc cagcgccgcc
     1021 gggaaatctt cggcccactg cagcgccgag gcgcactgga tcagcgcgtt ggcctccgcg
     1081 ggccggtcct gcacgcgccg ggccagctcg acgacggtct cggccgcctc gcgcgagcgc
     1141 acgaagtcac cgacgccgaa gaagaggtcc gcgcgcgcgg catgaatggc catcactgtc
     1201 gtcgcgggcg cgcgatcctc gagccgggcg gcggcggcca gcgcctctcc gtagagatcg
     1261 atcgcctgcc gcaggccgaa ggcccgggtc gccttctcgg cggctcgcat caggtaggtg
     1321 agcgcccgcc ccgcatcgtc ggtgtgcgag aagtgatgcg cgaggacctc gtagagctcg
     1381 ggcaggcggt cggcgtagag ctcctcgatg gcggtgccca cgagtcggtg tatctcgccc
     1441 cgccgctgaa cgagcaacga ggcgtaggcc acgtcctggg tcagcgcgtg cttgaaggcg
     1501 taggccagct cgggcagccg gcgccgctcg aggatgagct cgagcgcgct cagctcgcgc
     1561 agagcgccgt cggtcccctc cccgatctgc gagactcggc cgaccaggcg ccgcccgaac
     1621 tcgcgcccga tcaccgaggc gagctggagc gtgagcttgg gcgcctcggc cagccgatcg
     1681 atccgcgccg cgatcacgtc ctggacggtg ccgggaatcg cgatctgcga caccggacgg
     1741 gtgaggacga ggcgcccctc tgtccgatgg agcgcgccgc tctcctccag cgacctgacc
     1801 agctcctcgg tgtagaacgg attgccctcg gccttcgccg cgacgagaga ctggagctcg
     1861 gcgggaaaca ggtcgatccc caggatcgcc tccgcgatgc gcgcgctctc cccggtcgac
     1921 agcccggcgg gcacgacccg cgtgaagtag ctccgctccc cgaacggatt cacgtagccc
     1981 ggccggtagg tgagcacgag cagcacgcga atggccggca cgctctcgat gaggctcgtc
     2041 aggaactcct cg
//
LOCUS       DATMCV010000634         5530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_301413, whole genome shotgun sequence.
ACCESSION   DATMCV010000634 DATMCV010000000
VERSION     DATMCV010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301413"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..955
                     /locus_tag="VNU03_14575"
     CDS             65..955
                     /locus_tag="VNU03_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75743.1"
                     /translation="MLVAVVEAFLDPESGSETLQAASGSAFPVVIVNGPIGARIRLNA
                     GFGCLGPDPQRPAGASIGRALRLLQQNLGGALPGIGTMANYGGLRHTNVVFAEDEASL
                     PAGWAPHGTDRHGFAPGTSSISLVFANGATNIRRRGAKKETPEEDALQGMHRMADFMR
                     VPNMAGLAGYEHGTPGILMIPGVVAQTMVGLGWTKASIREFLWEHSRIPAEALRRAGC
                     PAWIEIDTSKVVRESLALDPWPITAQPENFVVVVAGGGHPTNSYWLQGYSPGVVGRRI
                     ELPAAFERLLAEAERDLGPE"
     gene            complement(970..1977)
                     /locus_tag="VNU03_14580"
     CDS             complement(970..1977)
                     /locus_tag="VNU03_14580"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75744.1"
                     /translation="MLERRAFLGVLAGSLFSAPPSAWAQLAGKVWRIGILSDSRPPTS
                     DEGIQAAGFAGVFGTRLRELGYVEGENLTLVWRRSPEGAAHQLDDLAADLVRLKVDVI
                     VATYPAAVFSARRATTTILVIMVHTPDPVRLGLVASLAHPGGNITGATSLSGDLTGKQ
                     LELLKQMLPRISRIAVVWNPDNPWHPIAVKGLEGNARSSRVRLQGLQVRAPDGFRPAF
                     AAMARERAQAVLFLADPLTFVHRAQLMDLAARQRLPTMGGPGEYADSGCLMTYWADTT
                     DLYGRAATYVDRILKGANPAALPIEQPTKYELVINLRTARTLGLTISPALLQRADRVI
                     E"
     gene            2028..2330
                     /locus_tag="VNU03_14585"
     CDS             2028..2330
                     /locus_tag="VNU03_14585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWP75745.1"
                     /translation="MELFIFARFHARAGNEAAVAEALLATVVPSREEPGCLSIHAFRS
                     IRDPRLFYIHSRWKDEAAFESHAGMPHTVRFIERVTPLIDHPLDVNRAHKMAETEG"
     gene            2452..2676
                     /locus_tag="VNU03_14590"
     CDS             2452..2676
                     /locus_tag="VNU03_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75746.1"
                     /translation="MIESVLLFFVGIGALLVSIVSILRLAWLAVLIPMLIAAGAAYGL
                     FVLNDHRSPRPNQPTRHTEPHPEKSRRMAA"
     gene            2696..2905
                     /locus_tag="VNU03_14595"
     CDS             2696..2905
                     /locus_tag="VNU03_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75747.1"
                     /translation="MILLLAGCATSTPDIRLRNPLTGQTFTCSGEAGRTGGRWWDEMA
                     KRTRDNCVKDLETQGYVIIKDPASR"
     gene            complement(2917..3762)
                     /locus_tag="VNU03_14600"
     CDS             complement(2917..3762)
                     /locus_tag="VNU03_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWP75748.1"
                     /translation="MSGAAQVALRFERDAASRTFLARKFATYPFFLAAPFHLDHTPHG
                     MLTAIMQSSAGGLYEGDRLVLSFDAEAGAEAHVTTQGATVAHPMPHGGEARHEVKIRA
                     AAGSLVEYLPDPLILLPTASVRSRVEVVVEVGSTVIVGDAFLAHDPAGRGRSFRRLAG
                     EASFRRPGASPFALERFDVTGESVEASWRGLGPRRCEAQGSLWVCAPGSPDELAATLS
                     RALEQVPGVYAGASTLSGGAGAMARLLAHDAVALGEGFRAAWRALRCRLTGLPPRSRR
                     QSGWL"
     gene            complement(3759..5468)
                     /gene="ureC"
                     /locus_tag="VNU03_14605"
     CDS             complement(3759..5468)
                     /gene="ureC"
                     /locus_tag="VNU03_14605"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016567920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWP75749.1"
                     /translation="MARMTRAAYAATYGPTTGDLVRLGDTSLLAEIERDHAVYGDECM
                     TGAGKVMRDGMGFDAHATSAAGALDMLVQNATIIDPVLGVVKADIGIRDGKIAAVGKA
                     GNPAIQSGVHPDLRCGPSTTIVHGDRLIVTPGGVEAHAHFLCPQQCEHALAGGLTTIV
                     GGTMGPHFDVSCAGPANLAIMLRAAEQYPLNFGFLGRGSGHDPAAIVESVAGGAIGVK
                     VHEDFGAMPATIDCALSVADRYDFQVQLHTDTINEAGYCEDTIAAIAGRTIHMYHVEG
                     AGGGHAPDIIRVTGVPHCLPSSTNPTNPFTRNALDEGLPMTMDAHCLNWQLPEDVAFA
                     ESRVRPQTMAAEDLLHDLGAISIFAADTQGMGRVTETVTNCWRLASVMKDRRGRLPAE
                     TTARADNERIKRYVAKYTINPARALGIDPYVGSIEPGKMADLVLWVPAFFGVKPLMVI
                     KSGFVVWSAMGDASGCIFPTEPVVQRPMWGAVGDAPSRLGAFFASALALEADVPRKLG
                     LRRRMLPIVSTRSLGKRDMLHNDACPEIVVDPSTHEVYADGQLLVCEPAASVPLSRRY
                     LLR"
BASE COUNT          841 a         1887 c         1983 g          819 t
ORIGIN      
        1 caccacggtc gagtcgtgcg ccgtcgcgct ggcgatggcc ggcgggcggc ccgagtagct
       61 gccggtgctc gtggcggtgg tggaggcctt cctcgatccc gagtcgggga gcgagacgtt
      121 gcaggccgcg tcgggcagcg cgttcccggt ggtgatcgtc aacgggccga tcggcgcgcg
      181 gatccggctc aatgccggct tcgggtgcct gggtcccgat ccgcagcgac cggccggggc
      241 cagcatcggc cgcgcgctcc gcctcctgca gcagaacctg ggcggcgccc tgcccggcat
      301 cggcaccatg gccaactacg gcgggctgcg ccacacgaac gtggtgttcg cggaggacga
      361 ggcgagcctg cccgcgggct gggccccgca cgggaccgac cgccacggct tcgcgcccgg
      421 gaccagctcg atctcgctcg tcttcgccaa cggggccacc aacatccgga ggcgcggcgc
      481 caagaaggag acgccggagg aggacgcgct ccagggcatg catcgcatgg ccgacttcat
      541 gcgcgtgccc aacatggccg ggctggccgg ctacgagcac ggcacccccg gcatcctgat
      601 gatcccgggg gtcgtggccc agaccatggt cggcctcggc tggaccaagg cctcgatccg
      661 cgagttcctc tgggaacatt cgcggattcc cgccgaggcg ctacgtcgcg ccggatgccc
      721 cgcgtggatc gagatcgaca cgagcaaggt ggtccgcgag agcctcgccc tggacccctg
      781 gccgatcacc gcccagccgg agaacttcgt ggtcgtcgtg gccgggggcg gccacccgac
      841 caacagctac tggctgcagg gctacagccc tggcgtggtc ggccggcgga ttgagctgcc
      901 ggccgccttc gaaaggctgc tcgcggaggc ggagcgcgac ctcggcccag agtagaggac
      961 gcgagccctc tactcgatca cccgatccgc ccgttgcagg agcgccggcg agatagtcag
     1021 gccgagggtc cgggcggtcc tgagattgat gaccagctcg tacttcgtcg gctgctcgat
     1081 gggcagggca gccgggttgg cacccttcag gatccgatcg acgtaggtcg ccgcgcgccc
     1141 gtagagatcg gtcgtgtcgg cccagtaggt catcaggcat ccactgtccg cgtattcgcc
     1201 cggcccgccc atcgtcggca ggcgctgcct ggccgccaga tccatcagct gggcgcgatg
     1261 gacgaaggtc agcgggtcgg ccaggaacag aaccgcctgc gcgcgttctc gcgccatcgc
     1321 ggcgaacgcg ggacggaagc cgtcgggcgc gcgcacttgc agcccctgca gccgcacgcg
     1381 cgacgatcgg gcattgccct cgagaccttt cacggcgatg gggtgccacg gattgtccgg
     1441 gttccagacg acggcaatcc tggagatccg tgggagcatc tgcttgagca gctcgagctg
     1501 cttcccggtc agatctccgc tgagcgaggt cgcgccggtg atgttgcctc ccggatgtgc
     1561 gaggctggcc acgaggccca gtcgaaccgg gtcgggagtg tgcaccatga tgacgagtat
     1621 cgtcgtcgtc gccctccggg cgctgaagac cgcggccggg tatgtcgcga cgatcacgtc
     1681 gaccttgagg cgcacgaggt cggctgcgag atcatcgagc tggtgggctg ccccctcggg
     1741 actccgccgc cacacgaggg tgagattctc gccctcgaca tatcccagct cccgcaatcg
     1801 tgtcccgaac acgccagcaa agcctgccgc ctgaatgccc tcgtcggacg tgggtggacg
     1861 ggaatccgaa aggatgccga tccgccacac cttccccgcg agttgggccc aggcggaggg
     1921 cggcgcgctg aagaggctac ccgcgagaac tcccaggaac gcccgccgtt cgagcatggc
     1981 gaatgatagc atccgcccca ggacgcactc ccgcgaggag gcaggagatg gagctgttca
     2041 tctttgcgcg gtttcacgcg cgggccggga acgaggccgc ggtggcggag gcgctgctcg
     2101 ccaccgtggt gccgtcgcgt gaagagccgg gctgcctgag catccacgca ttccggtcga
     2161 tccgagatcc gcgcctcttc tacatccatt cgcgctggaa ggatgaagcg gcgttcgagt
     2221 ctcacgccgg catgccgcac accgtgcgct tcatcgagcg cgtgacgccg ctcatcgacc
     2281 acccgctgga cgtcaaccgg gcacacaaga tggccgagac ggaaggatag tccgtgtcgg
     2341 taaaggttac cggccccgcc ggcccgcgtc tgaagcacaa ccggccatgt ttctcgtacc
     2401 ttggagcccg gcacacgcct tgcgaatccg taccgcaggg agggccgatc catgatcgag
     2461 agcgtgctgt tgttcttcgt cggaatcggg gcgctgctgg tttcgattgt ttcgattctc
     2521 cgcctggcgt ggctcgccgt cctgattccg atgctcatcg ccgccggggc cgcgtacggc
     2581 ctgttcgtgt tgaacgacca ccggtcacct cgtccaaatc agccgacgag gcatacggag
     2641 ccacaccctg agaaatcgcg acggatggcg gcctgagctc ggtgggaccg gactcgtgat
     2701 ccttctcctg gccggatgcg caacctccac gccggatatc cggctgcgga accccctgac
     2761 cggccagacg ttcacctgct cgggagaggc ggggcggacc ggcgggcgat ggtgggacga
     2821 gatggccaag cgaacccggg acaactgcgt gaaggacctg gagacccagg gctacgtgat
     2881 catcaaggat cccgcgagcc gctagcggaa tcccgatcag agccagccgg actggcggcg
     2941 gctcctcggc ggaaggccgg tgagccggca gcggagggcg cgccacgccg cgcggaagcc
     3001 ctcgccgagc gcgaccgcgt cgtgcgcgag caggcgggcc atcgccccgg cgccgccgga
     3061 cagggtggac gctcccgcgt agactcccgg cacctgctcg agcgcgcggc tgagcgtcgc
     3121 ggccaactcg tccggcgagc cgggagcgca cacccacagg cttccctggg cctcgcaccg
     3181 gcgaggaccc agcccacgcc agctggcctc cacgctctcg ccggtgacgt cgaagcgctc
     3241 gagggcgaac gggctagcgc ccggacgacg aaacgacgcc tcgcccgcca ggcgccggaa
     3301 cgaccgaccg cggcccgcgg gatcgtgggc caggaacgca tcgccgacga tgacggtcga
     3361 cccgacctcg accacgacct cgacgcggct ccgtacgctc gcggtgggca ggaggatcag
     3421 cgggtcgggc aggtactcca ccagtgaccc ggcggcggcc cggatcttca cctcgtgtcg
     3481 cgcctcgccg ccgtggggca tcgggtgagc gacggtggcg ccctgggtgg tgacgtgcgc
     3541 ctcggcgccc gcctcagcat cgaaggagag gacgagccgg tctccctcgt agaggccgcc
     3601 cgcgctcgac tgcatgatgg cggtgagcat gccgtggggg gtgtggtcca gatgaaaggg
     3661 tgccgcgagg aaaaacggat aggtcgcgaa cttccgggcg aggaatgtcc ggctcgccgc
     3721 gtcgcgctcg aagcggaggg ccacctgggc cgccccgctc atcggagcag gtatcgccgt
     3781 gagaggggca cggacgcggc cggctcgcag acgaggagct ggccgtcggc ataaacctcg
     3841 tgggtgctcg ggtcgacgac gatctccggg cacgcgtcgt tgtgcagcat gtcgcgcttg
     3901 ccgagcgagc gggtgctcac gatgggcagc atgcgccgac gaaggccgag cttgcgcggg
     3961 acgtcggcct cgagggccag ggcgctcgcg aagaaggctc ccagcctcga cggcgcgtcg
     4021 ccgaccgccc cccacatcgg gcgttggacg accggctcgg tggggaagat gcacccgctg
     4081 gcgtcgccca tcgccgacca gaccacgaag ccgctcttga tcaccatgag cggcttcacg
     4141 ccgaagaagg ccggcaccca caggaccaga tcggccatct tgccgggctc gatggacccg
     4201 acgtaggggt cgatgccgag agcgcgggcc gggttgatgg tgtacttggc gacgtagcgc
     4261 ttgatccgct cgttgtcggc cctcgcggtg gtctcggcgg gaagccgccc ccggcgatcc
     4321 ttcatgacgc tcgcgaggcg ccagcagttg gtgacggtct cggtgacgcg ccccatgccc
     4381 tgcgtgtcgg cggcgaagat cgagatggcg ccgaggtcat ggagcaggtc ctcggcggcc
     4441 atggtctgcg ggcgcacccg gctctccgcg aacgccacgt cttcggggag ctgccagttg
     4501 aggcagtggg cgtccatcgt catgggcaag ccctcgtcga gggcgttccg cgtgaagggg
     4561 ttggtcgggt tggtcgacga cggcaggcag tgcggcacgc cggtcacgcg gatgatgtcg
     4621 ggggcgtggc cacctcccgc cccttccacg tggtacatgt ggatcgtgcg gccggcgatg
     4681 gcggcgatgg tgtcctcgca gtagcccgcc tcgttgatgg tgtcggtgtg gagctgcacc
     4741 tggaagtcgt accggtcggc gacggacaac gcgcagtcaa tggtggcggg catcgcgccg
     4801 aagtcctcgt gcaccttcac gccgatggcg cctccggcca cgctctccac gatggcggcg
     4861 ggatcgtgac cgctgccgcg cccgaggaac ccgaagttga gcggatactg ctcggccgcc
     4921 cgcagcatga tggcgaggtt ggccgggccc gcgcagctga cgtcgaaatg cggccccatg
     4981 gtgccgccca cgatggtcgt gagcccgccg gccagcgcgt gctcgcactg ctggggacac
     5041 aggaagtggg cgtgggcctc gacgccaccc ggggtgacga tgaggcggtc cccgtgcacg
     5101 atggtcgtcg aggggccgca ccggaggtcg ggatggacgc ccgactggat ggccgggttg
     5161 ccggcctttc ccaccgccgc gatcttgccg tcgcgaatcc cgatgtccgc cttcaccacc
     5221 ccgaggacgg gatcgatgat ggtcgcgttc tgcaccagca tgtccagcgc gccggcggcc
     5281 gacgttgcgt gggcgtcgaa gcccatgccg tcgcgcatca ccttgccggc gccggtcatg
     5341 cactcgtcgc cgtacaccgc gtggtcgcgc tcgatctcgg cgaggagcga ggtatcgccc
     5401 aggcgcacga ggtctcccgt cgtcggtccg taggtggccg cgtaggcggc ccgcgtcatt
     5461 cgcgccatgg tccggtcctc ctcacgcgcc ctgaaagccg agctcgcggg cgcggcggag
     5521 ggccgcctcc
//
LOCUS       DATMCV010000635         4011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_436623, whole genome shotgun sequence.
ACCESSION   DATMCV010000635 DATMCV010000000
VERSION     DATMCV010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436623"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VNU03_14610"
     CDS             <1..742
                     /locus_tag="VNU03_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP75750.1"
                     /translation="SGSDLASIRTRAEKVDGGYRVNGTKIWTSNAHLSDYAIALFRTK
                     VVPDKKHEGLSQFLVDLKSPGISIRPIIDLAGGHHFNEVHFQDCLVPDDMLVGKDGEG
                     WKQVTTELGFERSGPERYLSSLQLVIELIREAGKEPGERAAALIGHFTAHLVTLRQMS
                     LSIATMLQAGQNPNLEAAVVKDIGTTFEQEIPEAVHALTGAEPRIGSGTEFEKTLGYL
                     VQHAPSFSLRGGTREVLRGIIARGLGLR"
     gene            813..1883
                     /locus_tag="VNU03_14615"
     CDS             813..1883
                     /locus_tag="VNU03_14615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP75751.1"
                     /translation="MNELQTILAETCTRLFTDHATTAVLEGAEKGQWPAALWQALEEN
                     GLTLPQVPEARGGAGGSWNDAFVVLMASGRFAAPVPLAETMLAGAILADAGLDAPLGP
                     MTVAPVHMDEGLTLARQGGGFALSGRATRVPWGARAEHVVVVADAGSAPMIALVAAGG
                     AKTEADASLAREPRDTLTWSGAPVAAAAPRRGRLGPHPTWAGGALVRSAQMAGGLEFL
                     LAQSVKYVTERKQFGRPLAAFQAIQQNLALLAGHTASAAMAAQQAFHVVERTGEGGDA
                     SFEIAVAKVRTGEASGLGAGIAHQAHGAIGFTYEHSLHFVTRRLWSWRAEFGAESHWS
                     VALGREVAARGGEALWPHMTSR"
     gene            1893..2786
                     /locus_tag="VNU03_14620"
     CDS             1893..2786
                     /locus_tag="VNU03_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP75752.1"
                     /translation="MHTLTLGDVSISRIVEIGRSSFPTAQMLPESDAGKIASHHRWIK
                     PHFFDDVTGDMGSRIQTWIVRTPRHTVLVDTGVGNDKDRGDNALWNRRSGGFLDDLAA
                     AGVTPEQVDLVLCTHLHVDHVGWNTRLVNGRWVPTFPRARYVFPGEEWEFWKYEEQAG
                     REKSGCIADSVVPVVEAGQAVMVSSTHQIDPWLAYEPWVGHTPGHAAVRAHTSAGDAV
                     FSGDLMHRVVQVAEPQWSSIFCYDGRQAAVTRKAFVERHAASGDLVLAAHFPHPGRIV
                     RDGASHRFEPVTLDAIAAARA"
     gene            2783..3919
                     /locus_tag="VNU03_14625"
     CDS             2783..3919
                     /locus_tag="VNU03_14625"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP75753.1"
                     /translation="MSAAPVLPLEGIRVVEVAQNLAGPMAAEILAHMGADVIKVERPE
                     GDDARKWGPPFWKGVSPGFLAVNANKRGITLDLKDPAAVAWLMEFLGSADVLVQNLRP
                     GSLEELGLGPDVLLARHPRLVYCSVWAFGSTGPLKLKPGFEPMVQAFSGLMMMNGDEG
                     GPPTRIGTSILDYGTGMWTAMGALAGLIQRQRTGRGCVVDASLYETGLAWLKGHFASF
                     RASGEVPERHRTGSQRVVPFQGFETKTGTIIVAAGNDRLFAKLAAVVGHPEWAKEERF
                     ATNAARVAHKPALLALLDAIFLTRTKGEWIDLLEAGGVPCGPIHGLPEAVAQPQAEVL
                     GIIQRLPGDDYDVVSLPISFDGHRPPIRRAPPRLGEHNAEIRGQ"
BASE COUNT          585 a         1412 c         1468 g          546 t
ORIGIN      
        1 ctcgggctcg gacctggcct cgatccgcac ccgggccgag aaggtggacg gcggctaccg
       61 ggtcaacggc accaagatct ggaccagcaa cgcgcacctc tccgactacg cgatcgcgct
      121 gttccgcacc aaggtggtgc cggacaagaa gcacgagggc ctctcccagt tcctggtgga
      181 tctcaagtcg cccggcatct cgatccgccc gatcatcgac ctggccggcg gccaccactt
      241 caacgaggtg cacttccagg actgcctcgt ccccgacgac atgctggtcg gtaaggacgg
      301 cgagggctgg aagcaggtga ccaccgagct cggcttcgag cgcagcgggc ccgagcgcta
      361 tctctcgtcg ctccagctcg tgatcgagct gatccgggag gcggggaagg agccgggcga
      421 gcgggcggcg gcgctgatcg gccatttcac cgcgcacctg gtgacgctgc gccagatgtc
      481 gctctcgatc gccaccatgc tccaggccgg gcagaacccg aacctggagg ccgcggtggt
      541 gaaggacatc ggcactactt tcgagcagga gatcccggag gcggtgcacg cgctcaccgg
      601 cgccgagccc cgcatcggca gcgggaccga gttcgagaag acgctcggct acctggtcca
      661 gcacgcgccg tcgttctcat tgagaggcgg gacccgcgaa gtccttcgcg gcatcatcgc
      721 ccgcggcctg ggtttgcggt aatgctgggc gaggcgcagc cgaaggcgag ccgagcgaat
      781 acaagaaatg aagtaagggg gttatgcctg tcatgaacga gctgcagacg atcctcgccg
      841 agacgtgcac gcgcctcttc accgatcacg ctaccaccgc ggtgctggaa ggggccgaga
      901 agggccagtg gcccgccgcc ctctggcagg ccctggagga gaacggcctc acgctgcccc
      961 aggtgcccga ggcccgcggt ggggcgggcg gctcctggaa cgacgccttc gtggtgctga
     1021 tggcctccgg ccgcttcgcg gcgccggtgc cgctggccga gacgatgctg gcgggcgcca
     1081 tcctggccga cgcggggctg gacgcgccgc tcgggccgat gaccgtcgca cccgtgcata
     1141 tggatgaagg cctcaccctg gcccggcagg gcgggggctt cgccctgagc ggccgcgcga
     1201 cacgcgtgcc gtggggcgcg cgcgccgagc acgtggtggt ggtggccgac gcgggcagcg
     1261 ctccgatgat cgcgctggtg gccgccgggg gcgccaagac cgaggccgac gcgagcctcg
     1321 cgcgcgagcc gcgcgacacc ctgacgtggt ccggcgcgcc ggtggcggcg gcggcgccgc
     1381 ggcggggccg gctcggcccg cacccgacgt gggcgggcgg cgcgctggtg cgctcggccc
     1441 agatggcggg cgggctcgag ttcctgctcg cccagtcggt gaagtacgtg accgagcgca
     1501 agcagttcgg ccggcccctc gccgcgttcc aggccatcca gcagaacctg gccctcctgg
     1561 ccgggcacac cgcgtccgcc gcgatggcgg cgcagcaagc ctttcacgtg gtggagcgca
     1621 ccggcgaggg cggcgacgcc tcgttcgaga tcgcggtggc caaggtgcgc accggcgagg
     1681 cctccggcct gggggccggc atcgcccatc aggcgcacgg ggccatcggg ttcacctacg
     1741 agcactcgct gcacttcgtc acccgccggc tctggtcctg gcgggccgag ttcggcgcgg
     1801 agagccactg gtcggtggcg ctgggccggg aggtcgccgc gcgcggcggc gaggcgctct
     1861 ggcctcacat gacgtcacgc taggagcccg acatgcacac gctcacgctc ggagacgtct
     1921 cgatctcgcg catcgtcgag atcggccgct cgtcgttccc caccgcccag atgctgcccg
     1981 agtccgacgc ggggaagatc gcgtcgcacc atcgctggat caagccccac ttcttcgacg
     2041 acgtgaccgg cgacatgggc tcgcgcatcc agacctggat cgtgcgcacc ccgcggcaca
     2101 ccgtcctggt cgacaccggc gtcggcaacg acaaggaccg cggcgacaac gcgctctgga
     2161 accggcggag cggcggcttc ctcgacgacc tggccgcggc gggggtcacc cccgagcagg
     2221 tggacctggt cctgtgcacg cacctccacg tcgaccacgt gggctggaac acgcggctcg
     2281 tgaacggccg ctgggtgccc acgttcccac gggcccggta cgtcttcccg ggtgaggagt
     2341 gggagttctg gaagtacgag gagcaggccg gacgcgagaa gtcgggctgc atcgcggaca
     2401 gcgtcgtgcc ggtggtggag gcggggcagg cggtcatggt gagcagcacg catcagatcg
     2461 acccgtggct cgcctacgag ccgtgggtcg gccacacccc gggccacgcc gcggtgcgcg
     2521 cgcacaccag cgcgggggac gcggtgttct cgggcgacct catgcaccgg gtggtgcagg
     2581 tggcggagcc gcagtggagc agcatcttct gctatgacgg gcgccaggcc gcggtgactc
     2641 gcaaggcctt cgtggagcgc cacgccgcct ccggcgacct cgtgctcgcg gcccacttcc
     2701 cgcatccggg tcgcatcgtc cgcgacgggg cgagccaccg cttcgagccg gtcacgctgg
     2761 acgcgatcgc ggcggcgcgc gcgtgagcgc ggcgccggtg ctgccgctcg agggcatccg
     2821 cgtcgtcgag gtcgcccaga atctcgccgg ccccatggcg gccgagatcc tcgcccacat
     2881 gggcgccgac gtcatcaagg tggagcggcc cgagggcgac gacgcgcgca agtgggggcc
     2941 gccgttctgg aagggcgtct cgccgggctt cctcgcggtc aacgcgaaca agcgaggcat
     3001 cacgctcgac ctgaaggatc ccgccgcggt cgcgtggctc atggagttcc tcggcagcgc
     3061 cgacgtgctg gtccagaacc tgcggccggg ctcgctcgag gagctcggcc tcgggcccga
     3121 cgtgctgctc gcgcggcacc cacggctcgt ctactgctcg gtgtgggcgt tcgggagcac
     3181 cgggccgctc aagctgaagc ccggcttcga gccgatggtg caggccttct cggggctcat
     3241 gatgatgaac ggcgacgagg gcgggccgcc gacgcgcatc ggcacctcga tcctcgacta
     3301 cggcaccggc atgtggaccg cgatgggcgc gctcgccggg ctgatacagc ggcagcgcac
     3361 cgggcgcggc tgcgtggtgg acgcctcgct ctacgagacc gggctcgcgt ggctcaaggg
     3421 ccacttcgcg agcttccgcg cctccggcga ggtgccggag cgccaccgca ccgggagcca
     3481 gcgggtggtg ccgttccagg gcttcgagac caagaccggt accatcatcg tcgccgcggg
     3541 gaacgatcgc ctcttcgcca agctcgccgc ggtggtcggc catccggagt gggcgaagga
     3601 ggagcgcttc gccaccaacg ccgcgcgcgt ggcccacaag cccgcgctgc tggcgctcct
     3661 cgacgccatc ttcctcaccc gcaccaaggg cgagtggatc gatctgctgg aagcgggcgg
     3721 cgtgccgtgc gggccgatcc acggcctgcc ggaggcggtg gcccagccgc aggcggaagt
     3781 cctcggcatc atccagcgcc ttcccggcga cgactacgac gtcgtctcgc tccccatctc
     3841 cttcgacggc caccgcccgc cgatccgccg cgctcccccg cgcctcggcg agcacaacgc
     3901 ggagattcgg ggtcagtagc cctaacggaa cgcggtgtag agcatcgtga ggccggagag
     3961 gagcatcagg gcgtccatca ggaggcggaa gcggtcgggg tgcaggcgca g
//
LOCUS       DATMCV010000636         2314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_697117, whole genome shotgun sequence.
ACCESSION   DATMCV010000636 DATMCV010000000
VERSION     DATMCV010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697117"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VNU03_14630"
     CDS             complement(<1..207)
                     /locus_tag="VNU03_14630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWP75754.1"
                     /translation="MPDAGQTRAYLALTLISALWGSYPAFAKLALAHFPPYVLVALRC
                     SLASAFLTVLLFRRGWEEFRELRAA"
     gene            231..701
                     /locus_tag="VNU03_14635"
     CDS             231..701
                     /locus_tag="VNU03_14635"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWP75755.1"
                     /translation="MADHILERRVWLARPRPEVFDFFADAANLPRVQPAWARPRFIAP
                     PPRLAAGAVLDFRVPALPVAWRVMIREFDPPHRFVDVQLRGPFARWEHRHRFMEGPRR
                     DEPGAAPGTWVEDRVTYRLPGGALGRLVHALGGGRRVRGLFDYRDRRLRELFGG"
     gene            complement(715..1692)
                     /locus_tag="VNU03_14640"
     CDS             complement(715..1692)
                     /locus_tag="VNU03_14640"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWP75756.1"
                     /translation="MERRVIPGQIDTVFEFDYSVQAEDMRSLYEKAKRDQWNASRDIA
                     WETPEPSDGRVLSDELVDIYGSKLWSSLSEKDQVELNRRIAAWRLSVLVYGEQGAMLA
                     CSQMVNIVTGTDEKFFQATQVMDEARHNEVLERYIQTRLGGTHYPMPENERVLFDTIL
                     TDSRWYIKTIALQLVAETFAVAIFKMMQEAAADPLLRQVCSRILLDESRHMGFGMLAL
                     PDIVKEASDAERREMEDFTCVALEKVLTGFFPIEAYRDVGFSPAQIEEAKAYRRDVAA
                     RNDYAVYRKFFKRDMHTSMVNNLARIGLLTERVRPRLSALGIELPSAAA"
     gene            1800..>2314
                     /locus_tag="VNU03_14645"
     CDS             1800..>2314
                     /locus_tag="VNU03_14645"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWP75757.1"
                     /translation="MNARRKATRAQILRAAADLFRERGYPASTVDHIAARLGMSKASL
                     YTHFRAKEEMLAAISRETIDAFTRDLSLVLGSDLGPEEQLRRVVRQHVRFVIANRSFL
                     TVFFGEEANLPARFARSLAAQKDRYDKGVERIVAEGIRRGVFRDVPPRLVVFGVLGMV
                     NWVHKWYNPRGR"
BASE COUNT          334 a          781 c          827 g          372 t
ORIGIN      
        1 ggccgcgcgc agctcgcgga attcctccca gccgcggcgg aagagcagca cggtgaggaa
       61 cgcggacgcg agcgagcagc ggagcgccac cagcacgtag ggcgggaagt gggcgagggc
      121 cagcttggcg aaggcggggt aggagcccca gagcgccgag atcagcgtga gtgcgaggta
      181 cgctcgcgtt tgaccggcgt cgggcacccg cctatgatag cgcggccgtc atggcggacc
      241 atatcctgga gcgtcgcgtg tggctggcgc ggccccgccc cgaggtgttc gacttcttcg
      301 cggacgcggc caatctcccg cgcgtccagc ccgcgtgggc gcggccgcgc ttcatcgcgc
      361 cgccgccgcg cctcgccgcg ggcgcggtgc tcgacttccg tgtcccggcc ctgccggtcg
      421 cgtggcgggt gatgatccgc gagttcgacc ctccccatcg attcgtcgac gtgcagctgc
      481 ggggaccgtt cgcgcgctgg gagcaccggc accgcttcat ggagggtccg cggcgcgatg
      541 agccgggggc ggcgcccggc acctgggtgg aggaccgggt cacctaccgg ctccccggcg
      601 gcgcgctcgg gcgcctcgtc cacgcgctcg gaggaggccg gcgcgtccgc ggactcttcg
      661 actaccgcga ccgccggctg cgcgagctgt tcggcggcta ggcgccgccc gcggtcaggc
      721 cgcggccgag ggcagctcga tgccgagggc ggacagccgc ggccgcaccc gctcggtcag
      781 gaggccgatg cgggcgaggt tgttcaccat cgaggtgtgc atgtcgcgct tgaagaactt
      841 ccggtacacc gcgtagtcgt tgcgggcggc cacgtcgcgc cggtaggcct tggcctcctc
      901 gatctgggcc gggctgaacc cgacgtcgcg gtaggcctcg atgggaaaga agccggtcag
      961 caccttctcc agcgccacgc aggtgaagtc ctccatctcc cggcgctccg cgtcgctcgc
     1021 ctccttgacg atgtcgggca gggccagcat gccgaagccc atgtgacgcg actcgtccag
     1081 caggatccgg ctgcagacct ggcggaggag gggatcggcg gcggcctcct gcatcatctt
     1141 gaagatggcc accgcgaagg tctcggccac cagctggagc gcgatggtct tgatgtacca
     1201 gcgggagtcg gtgaggatcg tgtcgaagag cacccgctcg ttctccggca tcgggtagtg
     1261 cgtgccgccg aggcgggtct ggatgtagcg ctccagcacc tcgttgtgac gggcctcgtc
     1321 catgacctgg gtggcctgga agaacttctc gtcggtgccg gtgacgatgt tcaccatctg
     1381 gctgcaggcg agcatcgcgc cctgctcgcc gtagaccagg accgagaggc gccacgccgc
     1441 gatgcgccgg ttgagctcga cctggtcctt ctcggagagg ctcgaccaga gcttgctacc
     1501 gtagatgtcg accagctcgt cggagagcac gcggccgtcg gagggctcgg gggtctccca
     1561 cgcgatgtcg cggctcgcgt tccactggtc gcgcttggcc ttctcgtaga ggctgcgcat
     1621 gtcctccgcc tgcaccgagt agtcgaactc gaacacggtg tcgatctgac cggggatcac
     1681 gcgacgctcc atacggcacc tcctccaaaa taagacccga taaaaccaat cagtatagtt
     1741 tctatactct tcggttttta tcacggggtc cggggggtgt caagtagact ggggcggcca
     1801 tgaacgcgag acgcaaggcg acccgggccc agatcctgcg cgcggccgcc gacctcttcc
     1861 gggagcgcgg ctaccccgcc tccacggtgg accacatcgc ggcccggctc ggcatgtcga
     1921 aggcgtcgct ctacacccac ttccgggcca aggaggagat gctcgccgcg atctcgcggg
     1981 agaccatcga cgccttcacc cgcgatctca gcctggtgct gggctcggac ctggggccgg
     2041 aggagcagct gcggcgggtg gtgcggcagc acgtgcggtt cgtcatcgcc aaccggtcct
     2101 tcctcaccgt gttcttcggg gaggaggcga acctgcccgc tcgcttcgcg cgctccctcg
     2161 cggcccagaa ggaccgctac gacaagggag tggagcggat cgtggccgag gggatccggc
     2221 gcggcgtgtt tcgggacgtt ccgccccgcc tggtcgtctt cggcgtgctc ggcatggtca
     2281 actgggtgca caagtggtac aacccgcgcg gccg
//
LOCUS       DATMCV010000637         4633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_901929, whole genome shotgun sequence.
ACCESSION   DATMCV010000637 DATMCV010000000
VERSION     DATMCV010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901929"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1216
                     /locus_tag="VNU03_14650"
     CDS             53..1216
                     /locus_tag="VNU03_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HWP75758.1"
                     /translation="MSVVRRSIPNAVPHLAGNEWKYVKECLDTNWVSSAGPFVDRFER
                     EVAQHVGVPHAVATVNGTAAIHVALLAAGVRPGDEVLVPAFTFVATANAVAYCGAHPV
                     FLDSEEASWGLDPARLAEFLERECRVDAGHVVNRRTGRIVRALLPVHLYGHPCDLDPL
                     LALARGYPMAVVEDSAEALGARYRGRAVGVDGLAGCLSFNGNKIITTGAGGMVLTRDE
                     ATAARVRRLTTQARGDAIEFVHDEIGFNYRLSNLHAALGVAQLEQLDSFVEDKRATAA
                     FYQQALGRVDGVYPFVEAAWARSTYWMPSLLLDEGRCPDVPALIRSLNAAGIQARPLW
                     RPLHLQPAFRDATAGPVAVAERLYARGLSLPCSVGITPEERQDVVDAVVQQLR"
     gene            1213..1986
                     /locus_tag="VNU03_14655"
     CDS             1213..1986
                     /locus_tag="VNU03_14655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP75759.1"
                     /translation="MSHAVLLRDRLRRYYTTYYRDVLGIPDWPALVNLREVEEQQERG
                     RLERLRRVLGGALGGRLLNVGCGTGGFNVAAEEAGVRPVGVDADAEAIAICALKREKA
                     GGAFVRAAAERLPFPDATFDVVYCFSAIEHVESVETTVAEMVRVTRPGGLVYVHTPNA
                     WSWYEGHYKLLWAPFLPAPLGRLYLRLRGRPSGYLATLRRLTPTGLRRAFRRVGVRDL
                     RFHDDAPPRESLGPLRVPIGLYYRLSGVSPFIELVARKP"
     gene            1983..3269
                     /locus_tag="VNU03_14660"
     CDS             1983..3269
                     /locus_tag="VNU03_14660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP75760.1"
                     /translation="MTSILFASEYYPPFAPGGAEWSTAAWAAALARRGHRVTVVTPNY
                     GAPPREEGDGVTVIRVPFPVKLRPGQGSAGWGVHRNPLFHVYFAWQLRRAARTAEAEI
                     IHAQGKATLVGAWLAGASLGRPVLVTIRDVGLLCPYGHCTQIESGRTFDCPIWSRYVG
                     QCVPFHLEHYRPGLGRWGRARVWSAAALAFLDQKILQIALRRIRGAIGVSRGVLALFD
                     PRLLPARSVVVHNLPPSIVEPSRTDALEVRNRLGIEPGPLVLYAGKLSIGKGTDVFLD
                     SLDGIRALAPGVRFALAGKGDMRVPAAPDLHRLGELGQADLFALYATADVVVVPSVWA
                     EPLSRVLLEAMRMGRPVVATRVGGTAEAVEDGVTGLLVDKGDPRALAKAIAELLLDPH
                     RREQMGTAARQRAARFFDEDRLVGALLAAYREAAAA"
     gene            3266..3931
                     /locus_tag="VNU03_14665"
     CDS             3266..3931
                     /locus_tag="VNU03_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP75761.1"
                     /translation="MKGGLPYYLGVSYRRKRVDADLAAATAHFTGRVLDVGGARQRGA
                     FRRPAGARWVVADIERGFRPDVGADVQALPFRDAVFDAIKATELLEHVPDVARALGEC
                     RRVLRPGGHLVITAPFLERLHGDPGDYSRYTRSMWERLLAAADLKPVTIDEQGGYFTH
                     LAGLLRFLVLRAPRPLRWAGYGFFPLLDLMTRLDASPRARRSDFAAFVGGYLIVAERP
                     PAS"
     gene            3939..>4633
                     /locus_tag="VNU03_14670"
     CDS             3939..>4633
                     /locus_tag="VNU03_14670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP75762.1"
                     /translation="MRICTPHCGIAPETTSGGETYERELLERLGRAGVKLEIILARGK
                     PHPVGVPNWTIHRFAIARGLRWYVAPLVVPAAIRRVHAEVGFDLLRVHSLRYIGPSAL
                     WARRRYRLDVPVVSHHHHLDPDPLNRLIEKRVVDGSDRVVVGSEFGRRQLREELGART
                     DHVSVVYYGVDAWFAPRPRREDLLARHRLAGRRVVLYFGGLKPRKNLGLLIEAWRALA
                     PRYPDTTLLIAGGG"
BASE COUNT          552 a         1663 c         1726 g          692 t
ORIGIN      
        1 ccgagacctt cgactggtac cggcagtggt gggagcgcga gggccggccg cgatgagcgt
       61 cgtgcggcga agcattccca acgcggtccc gcacctggcc gggaacgagt ggaagtacgt
      121 caaggagtgt ctcgacacca actgggtctc ctccgccggc cccttcgtgg accgcttcga
      181 gcgcgaggtc gcccagcacg tcggggtgcc ccacgcggtg gcgaccgtca acggtaccgc
      241 ggccatccac gtggccctgc tcgcggcggg cgtccggccg ggcgacgagg tgctggtgcc
      301 cgccttcacc ttcgtggcga ccgcgaacgc ggtggcctac tgcggggcgc acccggtctt
      361 cctcgacagc gaggaggcct cgtggggcct cgacccggcc cggctcgccg agttcctcga
      421 gcgcgagtgc cgcgtcgacg cgggccacgt cgtgaaccgg cgcaccgggc ggatcgtccg
      481 cgcgctgctc ccggtgcacc tctacggcca cccgtgcgat ctcgacccgc tgctggcgct
      541 cgcgcgcggc tacccgatgg cggtggtgga ggactcggcg gaggccctcg gcgcgcgcta
      601 ccgcgggcgc gcggtcggcg tcgacgggct cgcgggctgc ctctccttca acggcaacaa
      661 gatcatcacc accggtgcgg gcggcatggt cctcacccgc gacgaggcga cggcggcgcg
      721 ggtgcggcgg ctcaccaccc aggcgcgcgg ggacgcgatc gagttcgtcc acgacgagat
      781 cggcttcaac taccggttga gcaacctgca cgcggccctc ggagtggccc agctcgagca
      841 gctcgactcg ttcgtggagg acaagcgagc caccgccgcc ttctaccagc aggccctcgg
      901 ccgggtcgac ggggtttacc cgttcgtcga ggctgcgtgg gcccgctcga cctactggat
      961 gccctccctg ctgctggacg agggccgctg ccccgacgtc cccgcgctga tccggagtct
     1021 caacgcggcg ggcatccagg cgcgcccgct gtggcggccg ctccacctgc agcccgcctt
     1081 ccgcgacgcg acggccgggc ccgttgcggt ggccgagcgg ctgtacgcgc gcggcctgtc
     1141 gctgccgtgc tcggtcggga tcaccccgga ggagcgccag gacgtcgtgg acgcggtcgt
     1201 tcagcagctg cggtgagtca cgcggtcctg ctgcgggacc ggctccggcg ctactacacc
     1261 acctactacc gcgacgtgct gggcatcccc gactggcccg ccctggtcaa ccttcgagaa
     1321 gtcgaggagc agcaggagcg cgggcggctc gagcggctcc gtcgcgtgct gggcggcgcg
     1381 ctggggggcc ggctcctcaa cgtggggtgc gggaccgggg gcttcaacgt ggccgccgag
     1441 gaggcggggg tgcggccggt cggggtcgac gccgatgcgg aggccatcgc gatctgcgcg
     1501 ctcaagcggg agaaggcggg cggcgccttc gtgcgcgccg cggccgagcg gctgccgttc
     1561 cccgacgcga ccttcgacgt cgtctactgc ttctcggcca tcgagcacgt ggagtcggtc
     1621 gagacgacgg tggcggagat ggtgcgggtg acccggcccg gcggcctcgt gtacgtgcac
     1681 accccgaacg cgtggtcgtg gtacgagggc cactacaagc tcctgtgggc gccgttcctg
     1741 cccgcgccgc tcgggcgcct gtacctccgc ctgcggggcc gtccgagcgg gtatctcgcg
     1801 actctacggc ggctcacccc gaccggcctc cgccgtgcgt tcaggcgggt aggcgtgcgc
     1861 gatctgcgct tccacgacga cgcgccgccc cgcgagtcgc tcgggcccct gcgcgtgccg
     1921 atcggcctct actaccggct ctcgggcgtc tcgccgttca tcgagctggt ggcgcgcaag
     1981 ccgtgacgtc gatcctgttc gcctcggagt actacccgcc gttcgcgccc ggcggcgccg
     2041 agtggagcac cgccgcgtgg gccgccgcgc tggcgcggcg cggacatcgc gtgacggtgg
     2101 tgacgcccaa ctacggcgcg ccgccgcgcg aggaggggga cggggtgacc gtcatccgcg
     2161 tgccgtttcc ggtgaagctc cggccggggc agggcagcgc gggctggggc gtgcaccgga
     2221 acccgttgtt ccatgtctat ttcgcctggc agctgcgccg cgccgcccgc acggccgaag
     2281 ccgagatcat ccacgcgcag ggcaaggcga cgctggtagg ggcctggctg gcgggcgcca
     2341 gcctcggacg accggtgctc gtcacgattc gcgatgtggg actgctctgc ccgtacgggc
     2401 actgcaccca gatcgagagc ggcaggacct tcgactgccc gatctggagc cggtacgtgg
     2461 ggcagtgtgt cccgttccat ctcgagcact atcggccggg gctcggtcgg tgggggcggg
     2521 cgcgggtctg gagcgccgcg gcgctcgcct tcctcgatca gaagatcctc cagatcgccc
     2581 tgcggcgaat tcgcggcgcc atcggggtga gccgtggagt cctcgccctg ttcgatccgc
     2641 gtctcctgcc ggctcgctcg gtcgtcgtgc acaacctgcc gccgagcatc gtcgagccgt
     2701 cgcggacgga cgcgctcgag gtccggaacc gccttggaat cgaacccggt ccacttgtgc
     2761 tgtatgcggg gaagctgtcc atcgggaagg gcaccgacgt gttcctggat tcgctcgacg
     2821 ggatccgggc actggctccg ggcgtgcgct tcgcgctggc cggcaaggga gacatgcggg
     2881 tgccggccgc ccccgacctc catcgtctcg gcgagctcgg ccaggcggac ctgttcgcgc
     2941 tctacgcgac ggcagacgtg gtggtcgttc cgtccgtctg ggcggagccg ctcagtcgcg
     3001 tcctcctcga agccatgcgg atggggcggc cggtggtggc gacccgagtt ggaggcaccg
     3061 cggaggccgt cgaggacggc gtgaccgggc tgctggtgga caagggcgac ccgcgggcgc
     3121 tcgccaaggc gatcgcggag ctccttctcg atcctcaccg ccgggagcag atggggaccg
     3181 cggcgaggca gagggcggcg cggttcttcg acgaggatcg actggtcgga gccctgctcg
     3241 ccgcctaccg ggaggcggcc gcggcgtgaa gggcgggctc ccgtactatc tcggcgtgtc
     3301 ctaccggcgc aagcgcgtcg acgcggacct ggccgccgcg accgcgcact tcaccgggcg
     3361 cgtgctcgac gtgggcgggg cccggcagcg cggcgcgttc cggcggccgg ccggcgcgcg
     3421 ctgggtggtg gccgacatcg agcgcgggtt ccgccccgac gtgggtgccg acgtgcaggc
     3481 gctgccgttc cgcgacgcgg tcttcgacgc gatcaaggcc accgagctgc tcgagcacgt
     3541 gccggacgtg gcgcgggcgc tcggcgagtg ccggcgcgtg ctgcggcccg gcggccacct
     3601 ggtgatcacc gcgccgttcc tcgagcggct gcacggcgat ccgggagact actcgcgcta
     3661 cacccgcagc atgtgggagc gcctcctcgc cgccgctgat ctcaagccgg tgactatcga
     3721 cgagcagggc ggctacttca cgcacctggc ggggctgctc cgcttcctgg tgctgcgggc
     3781 cccgcgcccg ctgcgctggg ccggctacgg attcttcccg ctcctggatc tgatgacgcg
     3841 cctcgacgct tcgccccgcg cgcgccgctc ggacttcgcg gccttcgtgg gcggatacct
     3901 gatcgtggcc gagcgcccac ccgcgtcgtg agccgcgcgt gaggatctgc acgccccact
     3961 gcgggatcgc gccggagacg acctcgggcg gcgagaccta cgagcgcgag ctgctggagc
     4021 gcctcggccg ggccggcgtg aagctcgaga tcatcctggc ccggggcaag ccgcacccgg
     4081 tgggggtgcc caactggacg atccaccggt tcgcgatcgc gcgcggcctg cgctggtatg
     4141 tcgctccgct cgtggtgccg gcggcgatcc gccgcgtcca cgccgaggtc ggcttcgatc
     4201 tcctccgcgt ccactcgctg cgctacatcg gaccgtccgc gctctgggcg cgccggcgct
     4261 atcgcctgga cgtgccggtc gtgtcccacc accaccacct cgacccggac ccgctcaacc
     4321 ggctgatcga gaagcgggtg gtggacggat ccgaccgggt ggtggtgggc agcgagttcg
     4381 gccgccgcca gctccgggag gagctgggcg cgcggaccga ccacgtctcg gtcgtctact
     4441 acggggtcga cgcgtggttc gcgccgcggc ccagacggga ggatctgctg gcacgccatc
     4501 gcctcgccgg ccgccgggtc gtgctctatt tcgggggcct caagccccgg aagaacctcg
     4561 gtctgctcat cgaggcctgg cgcgcgctgg ccccgcggta tccggacacc acgctgctga
     4621 tcgcgggcgg ggg
//
LOCUS       DATMCV010000638         4039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_436721, whole genome shotgun sequence.
ACCESSION   DATMCV010000638 DATMCV010000000
VERSION     DATMCV010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436721"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VNU03_14675"
     CDS             complement(<1..373)
                     /locus_tag="VNU03_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75763.1"
                     /translation="MPSLRFKLLGGILIGLLAVIVALTMLLGRSYRVAAPPPESRAAV
                     APEPAAPRAETPPASPERPSRSKGQSDWTFFFRVGDTLTRMSDGTALGVVVRLERTHA
                     FPDGAGPAYVVRSQDQREAVFD"
     gene            665..901
                     /locus_tag="VNU03_14680"
     CDS             665..901
                     /locus_tag="VNU03_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75764.1"
                     /translation="MDTRVEEKVSLETVMKRGALVCVVAMGGVAVGLAIWALRRWHDE
                     REYQAWRASVSADPYRRDRNGYPVGAQLGFSRSR"
     gene            989..1798
                     /locus_tag="VNU03_14685"
     CDS             989..1798
                     /locus_tag="VNU03_14685"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HWP75765.1"
                     /translation="MSPAASGRFSMSSEAEDLARLRAWLREALEGLGVDRATRAELVL
                     AVGELCANSIEHAYEGRGGQPINVSVLGHPDRLVIEVEDFGRSFDADRYVAPDLDAVP
                     DHGLGIHLVHRIADSVAIDVKRDRGTRWTLVKYRPGLRSADGSMDGGAPPSRSGETMD
                     IEVTDSAGITLVAPRGDLDMAAADQMKRALIKLVDDGKRKVLVDLAQVGYVDSSGMGA
                     LVASLKHARTVGGELRLCALQDDVRAIFEMTRLNKAVTIHPSRSEALGAWA"
     gene            1789..2670
                     /locus_tag="VNU03_14690"
     CDS             1789..2670
                     /locus_tag="VNU03_14690"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HWP75766.1"
                     /translation="MGLTRRLLALALALGLGGTSVPARSVAAASPVRPLAGVRTWVVY
                     YGDAPEAARDLARFDVVVLDPHGHPPLPLVKVHGSLVLTYVSLGEVNTQHPEFAAIAG
                     EPWVLAANPSWPDARGLDVGAPAYERWLLDRVVPAALAGPVNGLFLDTADSALERERA
                     EPRRYAGAAQALERVLRELKRRNPRTLLLINGGLPLAERLVGVVDGVALESVWTDYDF
                     KAKAYRVRPTEEAESRAALLGRVAALGLPVFTLEYAAADGGLGPTELIRRSRARGFVP
                     YVSTIGLDRVSTHTLRP"
     gene            2667..>4039
                     /locus_tag="VNU03_14695"
     CDS             2667..>4039
                     /locus_tag="VNU03_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75767.1"
                     /translation="MSRRISRRALLGGSAAAAGARVLGVRGRAAAAGADASGELPRHV
                     LALYKSSELDNDVQGRRPKTATTNEVHGWAQMPLNWLGLMVEYHDIDQGLPDERVMAR
                     YRGIVTWYQTEEIAEPLAYLRWLGAQMRAGRRVVILGYLGALRDRRTLRSLDLAAVNE
                     ALAPSGLQFLGNWTGNQHVIELRRKDKRMMEFERPLPPGLPYYQQVVCRRPDSRAHLV
                     LARRDVPDSESHMVVTGPWGGFAAAEYAQFQFPGEGGAQWWIDPFAFFREALGIADWP
                     RPDTTTQCGRRIFYTHIDGDGLRNRSELRGGASSGQVILDEILSRYRLPTSVSVVVAE
                     VEPTLLGTPEFHELARAIYTLPNVEAGSHSYTHPLDWEKRTRSFAPAGVPYSVETETA
                     GAIRYMQERLLPPGKRVRLFQWSGSTRVTEDAIAVLDRLGVPNVNGGDTMRDREWPSY
                     TRVAPLMR"
BASE COUNT          560 a         1407 c         1505 g          567 t
ORIGIN      
        1 cgtcgaagac ggcctcgcgc tggtcttgcg agcggaccac gtaggcgggg ccggcgccat
       61 ccgggaaggc atgggtgcgc tcgagccgca cgacgacgcc cagcgcggtg ccgtccgaca
      121 tgcgggtcag ggtatcaccg acccgaaaga agaaggtcca gtcgctctgg cccttggagc
      181 gcgagggacg ctcgggagag gccggcgggg tctccgctcg cggcgccgcg ggctcgggcg
      241 caacggcggc tcgcgactcg ggtgggggcg ccgcgacgcg gtaggagcga cccagcagca
      301 tggtgagcgc gacgatcacc gcgaggagcc cgatcaggat gccgccgagc agcttgaacc
      361 tgagcgaagg catgtcgctc gacagagcaa gaagagggcc agtgggcccg gtaacaagga
      421 cggacattta aggcgcacgc gcggaccggg tctgacaaag tcttggcatc cgcctgacaa
      481 ggtctggagc gggagccgct ccggcctgcc caactggctg attttgggcg ggccgtccgc
      541 tggctcagct cttgctacgt cacgggggca tgctactgac tgcggtcctc acggtgttgt
      601 tcgtcggggt gagctcggcg atgctctggc tcgccatcgg cgcggactag ggggagaatc
      661 gctcatggac acgcgggtcg aggagaaggt ctcgctcgag acggtgatga agcgcggcgc
      721 gctcgtgtgc gtcgtggcga tgggtggcgt cgcggtgggg ctcgcgatct gggcgctccg
      781 gcgctggcac gacgagcggg agtaccaggc gtggcgggcg tccgtgagcg cggacccata
      841 ccgccgcgac cgcaacggct atccggtcgg cgcccagctc ggcttctccc gctcgcggta
      901 gcgcggctcc acgtcgcgcc gctcctgtcc cggggcggca ccgcacctgc ccggatccac
      961 acctccccac tgctatactg acggaagcat gagcccggcg gcatccggcc gcttctccat
     1021 gtcgagcgag gccgaggatc tggcccgcct gcgtgcgtgg ctccgtgagg ccctcgaggg
     1081 actgggagtg gatcgggcga cgcgggcgga gctcgttctc gcggtcggcg agctgtgcgc
     1141 caactccatc gagcacgcgt acgaggggcg gggcgggcag cccatcaacg tgtcggtcct
     1201 cggtcacccc gaccggctgg tcatcgaggt ggaggatttc gggcggtcgt tcgacgccga
     1261 ccggtacgtc gcgccggatc tcgacgcggt gccggaccac gggctcggca ttcacctggt
     1321 gcaccggatc gcggattcgg tcgcgatcga cgtgaagcga gaccggggaa cccgctggac
     1381 cctcgtcaag taccgaccgg gcctccgctc ggccgatggt tcgatggacg gcggcgcacc
     1441 gccgtcccgc tcaggagaga ccatggacat cgaagtgacc gactcggccg ggatcacgct
     1501 cgtcgctccg cggggtgacc tggacatggc ggcggccgat cagatgaagc gggcgctgat
     1561 caagctggtg gacgacggga agcggaaggt gctggtcgac ctggcgcagg tgggctacgt
     1621 cgacagctcg ggcatggggg cgctggtggc gtcgctgaag cacgcccgca ccgtgggcgg
     1681 cgagctgcgc ctgtgcgcgc tgcaggacga cgtgcgcgcg atcttcgaga tgacccggct
     1741 caacaaggcg gtcaccatcc atccctcccg ctcggaggcg ctcggggcgt gggcctgacg
     1801 cggcgcctgc tcgccctcgc cctcgccctg gggctcggcg ggacctcggt gcccgcgcgc
     1861 tcggtcgcgg ccgcgtcccc ggttcggccc ctcgcgggag tgcgcacctg ggtcgtctac
     1921 tacggcgacg caccggaggc ggcgcgggat ctggcgcgct tcgacgtggt cgtgctcgat
     1981 ccgcacggtc accccccgct gccgctcgtc aaggtccacg gctcgctcgt gctcacctac
     2041 gtgagcctgg gcgaggtgaa cacgcagcat cccgagttcg ccgcgatcgc gggagagccg
     2101 tgggtgctgg ccgccaaccc gagctggccc gacgcgcgcg gcctggacgt gggggcgccc
     2161 gcctatgagc gctggcttct cgaccgggta gtgccggccg cgctcgcggg accggtcaac
     2221 gggctcttcc tcgataccgc ggactcggcc ctggagcgcg agcgcgccga gccgcggcgc
     2281 tacgcgggcg cggctcaggc gctcgagcgc gtgctccgcg agctgaagcg ccggaacccg
     2341 cggaccctcc tgctgatcaa cggcggcctc ccgctcgcgg agcggctcgt gggcgtcgtc
     2401 gacggggtgg cgctcgagtc ggtgtggacc gactacgact tcaaggccaa ggcctaccgg
     2461 gtgcggccca ccgaggaagc ggagtcccgc gccgcgctgc tgggccgcgt cgccgcgctg
     2521 gggctgcccg tgttcacgct cgagtacgcg gcggccgacg gcggcctggg gcccacggag
     2581 ctcatccgcc gctcgcgggc gcggggcttc gtgccctacg tctccaccat cgggctcgac
     2641 cgggtctcca cccacacgct ccgaccgtga gcaggcggat ctcacgccgc gcgctgctgg
     2701 gcggctcggc cgccgcggcg ggggcccggg tgctgggagt ccgcggtcgg gccgcggccg
     2761 ccggagcgga tgcatccgga gagctgccgc gccacgtgct ggcgctctac aagtcgtccg
     2821 agctcgataa cgacgtgcag gggcggcgcc ccaagaccgc gaccacgaac gaggtccacg
     2881 ggtgggccca gatgccgctc aactggctcg ggctcatggt cgagtaccac gacatcgatc
     2941 aggggctgcc ggacgagcgg gtgatggcgc gctaccgcgg catcgtcacc tggtaccaga
     3001 ccgaggagat cgcggagccg ctcgcctacc tgcgctggct gggtgcacag atgcgggccg
     3061 gccgccgggt ggtgatcctc ggctacctgg gcgccctgcg cgaccggcgc acgctgcgca
     3121 gcctcgatct ggccgcggtc aacgaggccc tcgcgcccag cgggctccag ttcctcggca
     3181 actggaccgg caaccagcac gtcatcgagc tccgtcgcaa ggacaagcgg atgatggagt
     3241 tcgagcggcc gctgcccccc ggcctgccgt actaccagca ggtggtctgc cgccgcccgg
     3301 acagccgggc ccacctggtg ctggcccggc gggacgtgcc ggactccgag agccacatgg
     3361 tcgtgaccgg cccgtggggc gggttcgcgg ccgcggagta cgcgcagttc cagttccccg
     3421 gcgagggcgg cgcgcagtgg tggatcgatc cgttcgcctt cttccgggaa gcgctgggca
     3481 tcgcggactg gccgagaccc gacaccacga cccagtgcgg ccggcgcatc ttctacaccc
     3541 acatcgacgg cgacgggctg cgcaaccggt ccgagcttcg cggcggcgct tccagcggcc
     3601 aggtgatcct cgacgagatc ctctcgcgct accggctgcc caccagcgtc tcggtagtgg
     3661 tggcggaggt cgagccgacg ctgctcggca cccccgagtt ccacgagctg gcccgcgcga
     3721 tctacacgct gcccaacgtc gaggcgggca gccactccta cacccacccg ctcgactggg
     3781 agaagcgcac gcgcagcttc gcgccggcgg gagtgccgta ctcggtggag accgagaccg
     3841 cgggggcgat ccgctacatg caggagcggc tgctgccgcc gggcaagcgc gtgcgtctct
     3901 tccagtggtc cggatccacg cgggtcaccg aggacgcgat cgcggtcctc gaccgcctcg
     3961 gggtgccgaa cgtcaacggc ggggacacca tgcgggaccg cgagtggccg agctacaccc
     4021 gcgtcgcgcc cctgatgcg
//
LOCUS       DATMCV010000639         2623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_567150, whole genome shotgun sequence.
ACCESSION   DATMCV010000639 DATMCV010000000
VERSION     DATMCV010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567150"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..2495)
                     /locus_tag="VNU03_14700"
     CDS             complement(144..2495)
                     /locus_tag="VNU03_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWP75768.1"
                     /translation="MQIRELLDELLTSLETGPLISAVRHFEAKPAVFAPFPSSLDPRI
                     VEALKARGVEQLYSHQAKAVEAVAKGEHLVVVTPTASGKTLCYNLPVLQALVQQPEAR
                     VLYLFPTKALAQDQLAELTELAKSLPDMRMFTYDGDTPQDARRSVRARANLVLTNPDM
                     LHSGILPHHTKWVNLFQNLKYVVIDELHAYRGVFGSHLANVLRRLKRICRHYGASPQF
                     IMASATIANPGDLAQRLTGERVAELNESGAPTGEKTFMVYNPPVVNPELGIRAPYLGE
                     ASKLAARFLKKKVATIVFCQSRLSTEVVLASIKKMVEDKTGDSGIVRGYRGGYLPLRR
                     REVEQGLRSGEVLGVVSTSALELGIDIGHLDLAVLAGYPGTIASMWQQAGRAGRRTGE
                     SAAVMVATSSPMDQYLAAHPDYLFGAPPEHARINPENPFILINHVKCAAFELPLGADE
                     AFGGDVSSHLAALEDEGVLHRAGEHFHWSSETYPADHISLRTVTSDNFLVIDTTARDQ
                     QQVKRRQIIAEVDWKSAFAMVYPKAIYMVEGEPFEVQELHYREDEEKVAYVKKVMVDY
                     FTDAITAKGVWILQRFGRQETPGLLAEQGEVLVAEKVVGFKKIKMGTLENVGSGEVEL
                     PQQEMQTTSAWLTLDPELLAEISPRRDDLVDGLRALSHLLHSLAPIFLLCDIRDVGAW
                     LGDGSPSQAGQVVTRETMRAQLLQGEHFTPTIYLYDNQPGGIGLAERIFEVLPEMLAR
                     GLETLEGCGCRSGCPSCVGPVNEVGRQAKPVALKLLTRLVPRR"
BASE COUNT          383 a          908 c          913 g          419 t
ORIGIN      
        1 ctcgttctcc tcgaccgcgc cgtcgaggag cccctcgagc gagcggggcg cccccgcggg
       61 cggacggatc cggctcttcg cctcgatgcg ccggatgatg cgggtcagct cggtgaggtc
      121 gcggcccaac ggggtcggcc ccctcagcgc cgggggacga gacgcgtgag cagcttcagg
      181 gcgaccggct tggcctgccg gcccacctcg ttcaccggcc ccacgcacga cggacagccg
      241 ctccggcagc cgcagccctc gagcgtctcc aggccgcgcg cgagcatctc cgggagcacc
      301 tcgaagatgc gctcggccag cccgatgccg ccgggctggt tgtcgtagag gtagatcgtc
      361 ggggtgaagt gctcgccctg caggagctgg gcccgcatcg tctcgcgcgt caccacctgc
      421 ccggcctggg agggagagcc gtcgccaagc caggccccca cgtcgcggat gtcgcacagc
      481 aggaagatgg gggcgaggct gtgcagcagg tggctgagcg cgcgcaggcc gtcgaccagg
      541 tcgtcgcggc gcggcgagat ctccgcgagc agctccggat cgagggtgag ccaggcgctc
      601 gtggtctgca tctcctgctg cggcagctcg acctcgcccg agcccacgtt ctcgagcgtg
      661 cccatcttga tcttcttgaa gccgaccacc ttctcggcca ccagcacctc gccctgctcg
      721 gcgagcaggc ccggcgtctc ctggcgcccg aagcgctgca ggatccacac tcccttggcg
      781 gtgatcgcgt cggtgaagta gtcgaccatc accttcttca cgtaggcgac cttctcctcg
      841 tcctcgcggt agtggagctc ctgcacctcg aagggctcgc cctcgaccat gtagatcgcc
      901 ttgggataga ccatggcgaa ggcgctcttc cagtccacct ccgcgatgat ctgccgccgc
      961 ttcacctgct gttgatcgcg cgcggtggtg tcgatgacca ggaagttgtc ggaagtgacg
     1021 gtgcgcagcg agatgtgatc ggccgggtag gtctcggagc tccagtggaa gtgctccccg
     1081 gcgcggtgga gcacgccctc gtcctcgagc gcggccaggt gcgacgacac gtcacccccg
     1141 aacgcctcgt ccgcccccag gggcagctcg aaggccgcgc acttgacgtg gttgatgagg
     1201 atgaacgggt tctccgggtt gatgcgcgcg tgctcgggcg gcgccccgaa gaggtagtcc
     1261 gggtgcgcgg ccaggtactg gtccatcggc gagctcgtcg ccaccatcac cgcggcggac
     1321 tcgccggtgc gccggcccgc ccggcccgcc tgctgccaca tcgaggcgat ggtgcccgga
     1381 tagcccgcga gcaccgccag gtcgaggtgg ccgatgtcga tgccgagctc gagcgcgctg
     1441 gtcgagacca cccccagcac ctcgcccgag cggagcccct gctcgacctc gcgccggcgc
     1501 agcggcaggt agcccccgcg gtagccgcgc acgatgccgg agtcgccggt cttgtcctcc
     1561 accatcttct tgatcgaggc cagcaccacc tcggtggaga gccggctctg gcagaagacg
     1621 atggtggcga ccttcttctt gagaaagcga gccgcgagct tggaggcctc gccgaggtac
     1681 ggggcgcgga tgccgagctc cgggttcacc accggcgggt tgtagaccat gaaggtcttc
     1741 tccccggtgg gcgcgccgct ctcgttcagc tccgccaccc gctcgccggt cagccgctgc
     1801 gcgaggtcgc ccggattcgc gatggtggcc gaggccatga tgaactgcgg gctggccccg
     1861 tagtggcggc agatgcgctt gagccgccgc agcacgttgg cgaggtggct gccgaagacg
     1921 ccgcggtagg cgtgcagctc gtcgatcacc acgtacttga gattctggaa caggttcacc
     1981 cacttggtgt ggtgcggcag gatcccggag tgcagcatgt cgggattggt gagcacgagg
     2041 ttggcccgcg cccgcaccga gcgccgcgcg tcctgcgggg tgtcgccgtc gtaggtgaac
     2101 atccgcatgt cgggcagcga cttggccagc tcggtcaact ccgcgagctg gtcctgggcc
     2161 agcgccttcg tcgggaagag atacagcacg cgcgcttccg gctgctgcac gagcgcctgc
     2221 agcaccggca ggttgtagca gagggtcttg ccggacgcgg tcggggtcac caccaccagg
     2281 tgctcgccct tggccaccgc ctcgaccgcc ttggcctggt gcgagtagag ctgctccacc
     2341 ccgcgcgcct tcaaggcctc gacgattcgc ggatcgagcg aggagggaaa gggcgcgaac
     2401 accgccggct tggcctcgaa gtggcggacc gcgctgatca gcggcccggt ctcgagcgac
     2461 gtcaagagct cgtcgaggag ctcccggatt tgcatggctc atcctatgtt tagtgagcca
     2521 agttgtcaac tttgcgagcg cctcccgcga ccaggacggc caccgcggcg agctggagcg
     2581 cgagacaccc ggcgagggcc gcgcggtagt tgccgaatcc atc
//
LOCUS       DATMCV010000640         4965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_436858, whole genome shotgun sequence.
ACCESSION   DATMCV010000640 DATMCV010000000
VERSION     DATMCV010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436858"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VNU03_14705"
     CDS             <1..215
                     /locus_tag="VNU03_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75769.1"
                     /translation="SLLQLSVPHHLRGRAGGAWVVAIGFAPLGQLQIGALASLLGVSA
                     ALGVSGLALIALASATVFFYPRLRAL"
     gene            317..571
                     /locus_tag="VNU03_14710"
     CDS             317..571
                     /locus_tag="VNU03_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75770.1"
                     /translation="MAGASVSELARDIVVALIQTGKIQLDADPKKQGEWLGDLFKTIH
                     QKIIVQGAIEVLVEDQLKNFDLEDLRQLRELLDAEIAARS"
     gene            complement(576..2126)
                     /locus_tag="VNU03_14715"
     CDS             complement(576..2126)
                     /locus_tag="VNU03_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75771.1"
                     /translation="MSIEPGAQLRHELRTPLNHIIGYAELLLEELEAGDQPELAADLA
                     ALRADARQLLTLLNEVLARGQTGPPDLAAARGTLGPPLDRVRAAGESLHRRAAAAGAG
                     ARLADLDRIRAATGRLDELLGGAARSAPTAPGPAADGSAADPPAPPRAVILVVDDSEE
                     NRDMLARRLRRQGYEVQTAAGGRAALAALAESPVDLVLLDVMMPDLDGYAVLERLKAD
                     PTRRDIPVLMISALDDLDSVVRCIQLGAEDYLGKPFDPILLQARIGACLEKKRLHDQE
                     ARQRRELAALNQTLERRVADQVAQLERLGRLKRFFSPQLAELIVAGGAADPLAPHRRE
                     VTVVFVDLRGFTAFAESAEPEELMGVLREYHAAMGRLILAHEGTLERFTGDGMMVFFN
                     DPVEVPDPAPRAIRMAIAMRDQSAGLAARWQKRGWDLALGVGIAQGYATIGAIGFEGR
                     VDYGAIGTVTNLAARLCGEAGGGQILISARVAAAVEGMIDAESVGPLTLKGLARPVPV
                     WRVRGLTA"
     gene            complement(2137..2514)
                     /locus_tag="VNU03_14720"
     CDS             complement(2137..2514)
                     /locus_tag="VNU03_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75772.1"
                     /translation="MAKILLVEDNEMNRDMLSRRLARRGYEVAIAVDGEQGVAMARSE
                     APALILMDMSLPGLDGWEATRQIKAAPETRAIPVIALTAHAMSGDREKAVAAGCDDFD
                     TKPVDLARLLEKIEAQLGRASGA"
     gene            complement(2514..>4965)
                     /locus_tag="VNU03_14725"
     CDS             complement(2514..>4965)
                     /locus_tag="VNU03_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP75773.1"
                     /translation="VLYRFDGDLLHLVAHHHMPDELLAMLHGLYPMRPSRRHASGRAV
                     MSGAVVEIADTATDPEYQQDVAVKGGWRSMLAVPMLREGAPLGAIVIQRAEAGSFPES
                     QVTLLKTFADQAVIAIENVRLFKELQARTGELTRSVQELQALGEVSQVLSSTLDLETV
                     LNTIVSRATQIAGTDGGSVYEYDEGEEAFHLRATDNLDEEVVAMARRTPVPRGEGVLG
                     LMAATREPVQIPDIGAEGVYHSPLRAVLLKTGTRALMAVPLLRENVLIGGLTVNKKTP
                     GEFSPAVIELLKTFASQSAVAIQNARLFREIGDKSRQLELADRHKSEFLANMSHELRT
                     PLNAIIGYSEMLQEDAADLGAEQFTEDLGRINAAGKHLLELINAVLDLSKIEAGKMEL
                     YLETFEVAGLVRDIAAVIQPLAGKNANRLEVQCPPETGTMRADLTKVRQALFNLLSNA
                     CKFTERGTVTLAVAREASDGGEFIVFRVSDTGIGMTPEQLTRLFEAFSQADAATTRRY
                     GGTGLGLALSRRLCRMMGGDVTVESAAGRGSTFTIRLPVLVAQAAESPATAAEPAPLD
                     SVGIGTVLVIDDEAAVRDLMQRFLSREGFHVVTAAGGEEGLRLARELRPDAITLDVMM
                     PGMDGWAVLSALKADAGVADIPVVMLTIVDDKNLGYALGASDYLTKPIDRERLVKVLT
                     QHRRDRPVLVVDDDPGLRQLLRRMLEPEGYTVVEAENGRVALERLDDVRPSVILLDLM
                     MPEMDGFEFVAEFRRHEAWRVIPIVVVTAKDLSQDDRERLNGHVQKILQKGTHGREQL
                     LAEVHELVSASVARRRTA"
BASE COUNT          662 a         1831 c         1742 g          730 t
ORIGIN      
        1 agagcctgct ccagctgagc gtgccgcacc acctgcgcgg gcgcgcgggc ggcgcgtggg
       61 tggtggcgat cggcttcgcg ccgctcggcc agctccagat cggcgcgctc gcctcgctgc
      121 tcggggtgag cgcggcgctc ggggtgagcg ggctcgccct gatcgcgctc gcctccgcga
      181 ccgtcttctt ctatccgcgc cttcgcgcgc tctgaccggt ccggcgcggc gggcgcgttg
      241 ccggtgccgc cggaccttgg taggctcgga gaccatgagc caccgagagt ctgccggcgg
      301 gccggaagga gaagacatgg ccggagcgag cgtgtcggag ttggcgcggg acatcgtggt
      361 cgccttgatc cagaccggca agatccagct cgacgcggac cccaagaagc agggcgagtg
      421 gctgggcgat ctcttcaaga cgatccacca gaagatcatc gtccagggcg ccatcgaggt
      481 gctcgtggag gaccagctga agaacttcga cctcgaggac ctgcgccagc tgcgcgaatt
      541 gctcgacgcg gagatcgcgg cgcgcagcta ggcgctcacg cggtcagtcc gcgcacgcgc
      601 cagaccggca ccggccgggc caggcccttc agggtgagcg ggcccacgct ctccgcgtcg
      661 atcatcccct ccaccgccgc cgcgacgcgc gccgagatca ggatctgccc gccgccggcc
      721 tcgccgcaca ggcgggccgc caggttcgtg accgtcccga tcgcgccata gtcgacccgc
      781 ccctcgaagc cgatggcgcc gatggtggcg tagccctggg cgatgcccac cccgagggcc
      841 aggtcccagc cgcgcttctg ccagcgggcc gcgagcccgg cgctctggtc ccgcatcgcg
      901 atggccatgc ggatcgcccg cggggccgga tccggaacct cgaccgggtc gttgaagaac
      961 accatcatgc cgtcgccggt gaagcgctcg agcgtgccct cgtgggcgag gatgagccgg
     1021 cccatcgccg cgtgatactc gcgcagcacg cccatcagct cctcgggctc ggcggattcc
     1081 gcgaaggcgg tgaagccgcg caggtcgacg aagaccaccg tgacctcgcg gcggtgcgga
     1141 gccaggggat cggcggcgcc gccggccacg atcagctccg cgagctgcgg cgagaagaag
     1201 cgcttgagcc ggccgagccg ctcgagctgg gccacctggt cggcgacgcg acgctccagg
     1261 gtctggttga gcgcggcgag ctcgcggcgc tggcgcgcct cctgatcgtg gagccgcttc
     1321 ttctccaggc acgccccgat ccgggcctgg agcaagatcg gatcgaaggg cttccccaga
     1381 tagtcctcgg ccccgagctg gatgcagcgc accacgctat cgaggtcgtc cagcgcggag
     1441 atcatgagga ccgggatgtc gcgccgggtc gggtcggcct tgagccgctc cagcaccgcg
     1501 tagccgtcga ggtccggcat catgacgtcg aggagcacga ggtcgaccgg gctctccgcg
     1561 agcgcggcga gcgcggcgcg cccacccgcc gcggtctgca cctcgtagcc ctggcgccgg
     1621 agccggcggg cgagcatgtc gcggttctcc tcactgtcgt cgaccaccag gatcaccgca
     1681 cgcgggggcg cgggcgggtc ggcggcgctt ccgtcggcgg ccggccccgg ggccgtcggg
     1741 gcggaccgcg cggccccgcc cagcagctcg tcgagccgcc cggtggcggc gcggatgcgg
     1801 tccaggtcgg ccagccgcgc gcccgcgccg gccgccgccg cgcgccggtg caggctctcg
     1861 cccgcggcgc ggacccggtc gagcgggggg ccgagggtgc cgcgcgcggc cgcgagatcc
     1921 ggcggcccgg tctgcccgcg cgccaggacc tcgttcagca acgtgagaag ctggcgggcg
     1981 tcggcacgga gggcggcgag gtccgcggcc agctcgggct ggtcgcccgc ctccagctcc
     2041 tcgagcagca gctcggcgta gccgatgatg tggttcagcg gcgtgcgcag ctcgtggcgg
     2101 agctgggctc cgggctcgat gctcactgcc gcccgctcac gcgccggacg cccgcccgag
     2161 ctgcgcctcg atcttctcga ggagccgggc gaggtcgacg ggcttggtgt cgaaatcatc
     2221 gcagcccgcc gccaccgcct tctcccggtc gcccgacatg gcgtgcgcgg tgagcgcgat
     2281 caccgggatc gcgcgcgtct ccggggccgc cttgatctgc cgggtggcct cccagccgtc
     2341 cagtcccggg aggctcatgt ccatcaggat gagcgcgggc gcctccgagc gcgccatcgc
     2401 caccccctgc tcgccgtcga ccgcgatggc gacctcgtag ccgcgccggg cgagacggcg
     2461 ggagagcatg tcgcggttca tctcgttgtc ctcgacgagc aggatcttgg ccatcaggcg
     2521 gtcctccggc gggccacgct ggccgacacc agctcgtgca cctcggcgag gagctgctcg
     2581 cggccgtgcg tccccttctg gaggatcttc tgcacgtgac cgttcagccg ctcgcggtcg
     2641 tcctgggaga ggtccttggc ggtgaccacc acgatcggga tcacgcgcca cgcctcgtga
     2701 cggcggaact cggcgacgaa ctcgaagccg tccatctccg gcatcatcag gtcgagcagg
     2761 atgacgctcg gccggacgtc gtccaggcgc tcgagcgcga cgcgcccgtt ctccgcctcc
     2821 accaccgtgt agccctccgg ctcgagcatc cgccggagga gctgccggag ccccgggtcg
     2881 tcgtccacca cgagcaccgg ccggtcccgt cggtgctggg tgagcacctt caccagccgc
     2941 tcgcggtcga tcggcttggt caggtagtcg gaggcgccca gcgcgtagcc caggttcttg
     3001 tcgtcgacga tggtcagcat gacgaccggg atgtcggcca cccccgcgtc ggccttgagc
     3061 gccgacagca ccgcccagcc gtccattccc ggcatcatga cgtcgagggt gatcgcgtcg
     3121 ggccggagct cgcgggccag gcgcaggccc tcctcgccac cggccgcggt gacgacgtgg
     3181 aacccttccc ggctcaggaa ccgctgcatc aggtcgcgca ccgccgcctc gtcgtcgatc
     3241 accagcaccg tgccgatacc cactgagtcg agcggggccg gctcggcggc cgtcgccggc
     3301 gattcggccg cctgggccac gagtaccggc aggcggatgg tgaacgtgct tccccgcccc
     3361 gccgcgctct cgaccgtgac gtcgcccccc atcatccggc agagccgccg gctcaacgcc
     3421 aggccgagcc cggtcccgcc gtagcggcgc gtggtggccg cgtcggcctg ggagaacgcc
     3481 tcgaagaggc gagtgagctg ctccggggtc atcccgatgc cggtgtcgct tacgcggaac
     3541 acgatgaact ccccgccgtc gctggcttcc cgcgccaccg cgagcgtcac cgtcccccgc
     3601 tcggtgaact tgcacgcgtt gctcagcagg ttgaagaggg cctggcgcac cttggtgaga
     3661 tctgcgcgca tcgtcccggt ctccggcgga cactggacct ccaggcgatt ggcgttcttc
     3721 ccggccaggg gctggatcac cgccgcgatg tcccggacca ggccggccac ctcgaaggtc
     3781 tcgaggtaca gctccatctt gcccgcctcg atcttggaca gatcgagcac cgcgttgatc
     3841 agctccagca ggtgcttgcc cgccgcgttg atccgcccga gatcctcggt gaactgctcc
     3901 gcgccgaggt cggcggcgtc ctcctgcagc atctcgctgt agccgatgat cgcgttcagc
     3961 ggggtgcgca gctcgtgcga catgttggcg aggaactcgg acttgtggcg gtcggccagc
     4021 tcgagctggc ggctcttgtc cccgatctcc cggaagagac gcgcgttctg gatcgccacc
     4081 gccgactggc tcgcgaaggt cttgaggagc tcgatgaccg cgggcgagaa ctctcccggc
     4141 gtcttcttgt tcacggtcag accgccgatc agcacgttct cgcgcagcag gggcaccgcc
     4201 atcagagcgc gggtccccgt cttcagcagc acggcgcgga ggggactgtg gtacacgccc
     4261 tccgccccga tgtccgggat ctgaaccggc tcgcgcgtcg ccgccatcaa cccgagcacg
     4321 ccctcgcccc ggggcaccgg cgtcctccga gccatcgcga ccacctcctc gtcgagattg
     4381 tcggtggcgc gcaggtggaa cgcctcctcc ccctcgtcgt actcgtacac cgagcccccg
     4441 tcggtgcccg cgatctgggt ggcgcgggac acgatcgtgt tgagcacggt ctcgaggtcg
     4501 agggtcgagc tgaggacctg gctgacctcg ccgagcgcct gcagctcctg gaccgagcgg
     4561 gtgagctcgc cggtccgcgc ctgcagctcc ttgaacaagc ggacgttctc gatggcgatc
     4621 accgcctggt ccgcgaacgt cttgagcagc gtgacctggc tctccgggaa cgagccggcc
     4681 tccgcccgct ggatcacgat cgctccgagg ggagctccct cccgcagcat gggcaccgcg
     4741 agcatgctcc gccacccccc cttcaccgcc acgtcctgct ggtactccgg atcggtcgcg
     4801 gtgtcggcga tctcgaccac cgcgccgctc atgaccgccc ggcccgacgc gtggcgccgg
     4861 ctcgggcgca tcgggtagag cccgtgcagc atcgcgagga gctcgtccgg catgtggtgg
     4921 tgggcgacca ggtgtagcag gtccccgtcg aaccggtaca gcacg
//
LOCUS       DATMCV010000641         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_368634, whole genome shotgun sequence.
ACCESSION   DATMCV010000641 DATMCV010000000
VERSION     DATMCV010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368634"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VNU03_14730"
     CDS             <1..436
                     /locus_tag="VNU03_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HWP75774.1"
                     /translation="VAVSESSTGGLISAALLSIPGASAYFMGGGVIYTQTARRGLLQV
                     SDDAMRGIRSSTEAAALLMARTVRERLGTTWALAETGAAGPTGNRYGDAAGHSCFAVV
                     GAVETARTLETGRGDREANMWAFTRAALELLDACIQQAGDKS"
     gene            433..1476
                     /locus_tag="VNU03_14735"
     CDS             433..1476
                     /locus_tag="VNU03_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWP75775.1"
                     /translation="MSDKTIRVGFVGAGANTRKHHLPKLKAQPGVELVAVANRSKESG
                     DRVAREFGIGRVHDDWQEIVEASDIDAVCIGTWPYMHSVMSIAALERGKHVLCEARMA
                     MNAGEGRRMLEASRKAPHLIAQLVPAPHTLEVDATIQTLLGEGFVGEVLAVELQASGG
                     RFVEPDAPLTWRQDIGLSGHNILNMGIWYEAMMRWLGPAQRVTAMTKVAVPHRKDAAG
                     ASHEVRVPDHVDILAQLRGGAVGHLRFSSVTGLAPASEAWIFGSEGTLRLEADTLRLT
                     GGRRGESALREIPVPPKTRIGWRVEEEFVNAIRGREKIARTSFEDGVRYMEFTDAVTR
                     SASTGQTVDVKDL"
     gene            complement(1533..>2542)
                     /locus_tag="VNU03_14740"
     CDS             complement(1533..>2542)
                     /locus_tag="VNU03_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75776.1"
                     /translation="FDRLDIKCGGDAVGAARAVLQTGEYDFAYYVLIEEEVLRRIEQG
                     GKGRVMTIPSSAVSFIQCNQTDPLREVDGERSSLKAPHPLLTDQAVRSALSLTVDRTS
                     IQEHLVGRTGQITANFLNAPDRFRSQNSVWEFSIEKANDVLEQAGYLRGPDGVRAKDT
                     RRLRLVFQGAANATVQKIQTVIKQGAARAGIEMEVKAIPASVFFSSDVSNVDTNVRFH
                     ADLQTYTVFTGLDPQLFMAQFASWEIPTKENGWNGRNISRWRNAEFDRLWRKAENEMD
                     PVKRAALFIKMNDMVVQSAVVIPVTWRNVLHAAVNSLAGIEPNSWDSIFGRIAYWYRL
                     G"
BASE COUNT          371 a          889 c          897 g          385 t
ORIGIN      
        1 ggtggcggtc tccgagtctt cgaccggcgg cctcatctcc gccgcgctgc tctcgatccc
       61 cggcgcgtcc gcgtacttca tgggcggcgg cgtcatctac acccagaccg cgcgccgcgg
      121 cctgctccag gtgtccgacg acgcgatgcg cggcatccgc tcgagcaccg aggcggccgc
      181 gcttctgatg gcccgcacgg tgcgcgagcg gctcggcacc acgtgggccc tcgccgagac
      241 cggagccgcg ggcccgaccg gcaaccgcta cggggacgcg gccgggcact cctgcttcgc
      301 ggtggtgggt gcggtggaga ccgcccgcac gctcgagacc gggcgcggcg accgcgaggc
      361 caacatgtgg gccttcaccc gcgccgccct cgagctcctc gacgcctgca tccagcaggc
      421 cggagacaaa tcatgagcga caagacgatc cgcgtcggct tcgtgggagc cggcgccaac
      481 acccgcaagc accatctccc caagctcaag gcccagcccg gcgtggagct ggtcgcggtc
      541 gcgaaccggt ccaaggagtc gggcgatcgg gtcgcccgcg agttcggcat cggccgcgtg
      601 cacgacgact ggcaggagat cgtggaggcg tccgacatcg acgcggtgtg catcggcacg
      661 tggccctaca tgcacagcgt gatgagcatc gcggcgctcg agcggggcaa gcacgtgctc
      721 tgcgaggcgc gcatggcgat gaacgcgggc gagggccggc gcatgctcga ggcctcgcgc
      781 aaggcgccgc acctcatcgc ccagctcgtg cccgcgcccc acaccctcga ggtggacgcg
      841 accatccaga ccctgctcgg cgagggcttc gtcggcgagg tgctcgcggt cgagctgcag
      901 gcctcgggcg gccgcttcgt cgagccggac gcgccgctca cctggcggca ggacatcggg
      961 ctgagcggcc acaacatcct caacatgggg atctggtacg aggcgatgat gcgctggctc
     1021 ggcccggccc agcgggtgac cgcgatgacc aaggtcgcgg tgccgcatcg caaggacgcg
     1081 gcgggcgcct cgcacgaggt gcgggtgccc gatcacgtgg acatcctggc ccagctccgc
     1141 gggggggcgg tcggtcacct gcgcttcagc tcggtgaccg ggctggcccc cgcctccgag
     1201 gcctggatct tcggtagcga gggcaccctg cggctcgagg ccgacacgct ccggctcacc
     1261 ggcggccgtc gcggcgagtc cgcgctgcgc gagatcccgg tgccacccaa gacccgcatc
     1321 ggatggcggg tggaggagga gttcgtcaac gccatccggg gccgcgagaa gatcgcgcgc
     1381 acctcgttcg aggacggggt gcgctacatg gagttcaccg acgcggtcac ccggagcgcc
     1441 agcaccggcc agaccgtcga cgtgaaggac ctctaaccgc ttccccctct cccctcaggg
     1501 gagagggcag ggtgaggggt ggttcgaggc tactacccga gccggtacca gtaggcgatc
     1561 cggccgaaga tcgagtccca cgagttgggc tcgatcccgg ccaggctgtt gacagcggcg
     1621 tggagcacgt tgcgccacgt gaccgggatc accaccgcgc tctgcaccac catgtcgttc
     1681 atcttgatga agagcgcggc ccgcttgacc gggtccatct cgttctctgc cttgcgccac
     1741 agccgatcga attccgcgtt gcgccagcgg gagatgttgc ggccgttcca cccgttctcc
     1801 tttgtcggga tctcccacga ggcgaactgg gccatgaaga gctggggatc gaggccggtg
     1861 aacacggtgt aggtctggag atcggcgtgg aagcggacgt tggtgtccac gttgctcacg
     1921 tcggacgaga agaacacgga ggccgggatg gccttcacct ccatctcgat cccggcccgg
     1981 gccgccccct gcttgatcac ggtctggatc ttctgcaccg tggcgttggc cgcgccctgg
     2041 aataccaggc ggaggcgccg ggtgtccttg gcccgcaccc cgtcggggcc gcggaggtag
     2101 ccggcctgct ccagcacgtc gttggccttc tcgatgctga actcccacac gctgttctgg
     2161 gagcggaagc ggtccggcgc gttgaggaag ttggcggtga tctgcccggt gcggccgacg
     2221 aggtgctcct ggatcgaggt gcggtccacc gtcaggctca gcgcgctgcg caccgcctgg
     2281 tcggtcagga ggggatgcgg ggccttgagg ctcgagcgct cgccgtccac ctcgcgcaat
     2341 gggtcggtct ggttgcactg gatgaagctc accgcgctgc tcgggatcgt catgacccgg
     2401 cccttgccgc cctgctcgat ccgccggagc acctcctcct cgatcagcac gtagtacgcg
     2461 aagtcgtact cgccggtctg cagcaccgcg cgcgccgcgc ccaccgcgtc gccgccgcac
     2521 ttgatgtcga ggcggtcgaa ga
//
LOCUS       DATMCV010000642         8669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_368661, whole genome shotgun sequence.
ACCESSION   DATMCV010000642 DATMCV010000000
VERSION     DATMCV010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368661"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VNU03_14745"
     CDS             complement(<1..248)
                     /locus_tag="VNU03_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75777.1"
                     /translation="MKIPSRRLRIAGLAAAALLLAAEPSPSLGQVVVDSRSICAAIAD
                     PKVQHNAEQTTPALTRFPVVVHYMKHSSEGSGPDSAVRG"
     gene            complement(245..1318)
                     /locus_tag="VNU03_14750"
     CDS             complement(245..1318)
                     /locus_tag="VNU03_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75778.1"
                     /translation="MRRRILVIGAGTGASNNLIRSLKAGDPSLFVVGAHAERFVVKKS
                     AADRNYLMAPPGGAAFIRSVRGIVDAERIDLIIPTGDADVDALSERRQRFPGRLFLPS
                     RAIIRLCQDKYRLTTFLRQRGVPAPDTYPVGALSRIEELFESLHGRVPLWCRARTGTR
                     SLGAAPVTNPAQARGWISHWEELRGVPATSFTLSEYLPGRDFLCQSLWRRGALVLANT
                     FERLAYFGGENSPSGVSSLSSLAKTVIDRRILDTCVRAIRALSRTASGAFSVDLKEDR
                     DGVPCITEINAGRFFIGMTAFDHVGKHNMPHTYVKLALGEPVGLREEYDAVEDYYLVR
                     DLDTLPGVFHAAELFDGIREVPT"
     gene            1459..2370
                     /locus_tag="VNU03_14755"
     CDS             1459..2370
                     /locus_tag="VNU03_14755"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWP75779.1"
                     /translation="MTLSPSDREGGPPGALRAFECRYLRALRGWHLACPWIRAEPPLH
                     PSRRPRHRNHPRPPRGARPGRAGRYLIVNADDLGADNGTTRGIIEAHERGIVTSASLM
                     VDMPGARAAARMAREHPRLGVGLHVNFTAGPRALDPGDLRAQQRELERQLDVFGDLTG
                     ALPTHIDSHHHVHRRADVASLFVELARDRGIPLRGFGGITYVGNFYGQWEHGTTDLRH
                     ISPDYLIALLRSIAPGFSELACHPGDRDARFDPVYDWQRQVELESLTNPRVTAAAAQE
                     GIRLVDYREYRRLAPAAGASLPRYSTT"
     gene            complement(2355..3644)
                     /locus_tag="VNU03_14760"
     CDS             complement(2355..3644)
                     /locus_tag="VNU03_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HWP75780.1"
                     /translation="MGAAMWEAEAETRPREALEQETVEGMRRTLGHALTNPAWRRRLG
                     RVEPGDIRSAADWALLPFLTKDELRDAYPFGLACAGPGRVLRVHMSSGTTGNPVVNPF
                     TRADVEQWAAVMARCYTAAGVGARDVIQITPSFGLFTGGFGFHYGAERIGAMVVPIGA
                     GRTSLQLQLMRDLGTTVLAGISTYPLRILEVAREERFDLGSLKLRVAILGSEMWSDDL
                     RRLIERELSLTTFDIIGMTETGGPGLGIDCAAREGIHVWEDHYFCEVVDPATGRPRAD
                     GEEGELVVSTLTREGLPMIRYRTRDLTRVLSRGPCACGRTHLRLDRLHGRTDDMVIFR
                     GVNFYPGQIESLLLKQPGVGHEYQIVLDRAPAGSDRLVLLVEAREGFAETGLPRLRQE
                     LREVLNLSPEITLLAQGALPRPPGKAVRVVDRRQVVE"
     gene            complement(3644..5938)
                     /locus_tag="VNU03_14765"
     CDS             complement(3644..5938)
                     /locus_tag="VNU03_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWP75781.1"
                     /translation="MSFSVIGKDTPKRDGAEKVTGHTQFLHDLTLPRLAHGAILRTRH
                     PHARVRRIDTSAAEALPGVVAVITADRVEQHPFGFAKDHLPLKGGAADGGKVRCIRDE
                     IAAVAALTEEIAQEAVRLIRVDYEELPAVFDPASALRPGAPLVHGERGGNLVNLRYQF
                     AHGDVESAFARAHVVVEGEYRLNYVTTACMGTMVAIAAWSPPESPDAALTMWSTTQVP
                     FLYQRDLAEALGITGDRVRVMQPPVGGNFGRGLDLYPIDIITALLARRAGRPVKIVFE
                     RIEEFIASPTREPCVIHLRTAADRDGRLLARDARVVIDNGAYVSWGSTTPYVMLSTTA
                     GLYRVPAVRFDTSIVYTNNPYSGSMRGYGNLESTFAIEAQMDDLAERLGLDRVEIRRR
                     NATRSGDVNPQGNIITSCAMTECLDAVEREMSRAAGEAPRPGWRRGVGYAAMFHVGGG
                     ARVYRSDGCGAILKLDDFGKVSLLTGASEIGQGSETVLAMIVAEELGVPLGRIEVVNS
                     DTSIKPWDVGVHASRTTFIAGNAALQAARILKGKLLALAASVMDERPDDLELAAGRVR
                     VKGHPERGMEYDRVVRAGHFREGGRTLVAEAFYDPPNEMLSKDLRGNVSAAYGFAAQA
                     ALVEVSLATGQVRVLKVASAHDVGRALNPLAAEGQIHGGIHMGLGYALSEELRIEEGR
                     VLNPQFMEYALLTAADMPEIVIHLIETVDQAGPFGAKGLGESGVIPVAAAVANAVKDA
                     VGVRLTELPMTPERVFRALAARPS"
     gene            complement(5972..6520)
                     /locus_tag="VNU03_14770"
     CDS             complement(5972..6520)
                     /locus_tag="VNU03_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP75782.1"
                     /translation="MTTSPPVSFSHFGIYVTDLAGMEDFYTRVLGLLVSDRGQVPGGS
                     NLVFLSRDPDEHHQIVLATGRPPGVEFNVVNQMSFKVPGLVDLKAMHARAREEGIKQF
                     RVVTHGNAWSVYFADPEGNRLEVFVDTPWHTPQPVVEPFDIEAPVETIERETEALCRN
                     RPGFMSRAEWRAAQVTRMATAR"
     gene            6634..7971
                     /locus_tag="VNU03_14775"
     CDS             6634..7971
                     /locus_tag="VNU03_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWP75783.1"
                     /translation="MGATQRYVVIDPATGKIDRRIFSDQAVYDDEMERIFGRAWLMIG
                     HESLVPAADDFFHTYMGEDPVILTRDAQGRLHALLNMCRHRGNRVVRCDDGNANRFMC
                     TYHGWTYKNDGTLDHVPGKAEAYYDALDRDSLGLVQARVETYAGIVFATWAKDAPSLE
                     AYLGDARWYLDTVFNRRDGGMQALGPMKWLEPVNWKTMVDNCSDNYHVPTTHLSSARV
                     QTKFLGRPRLSHKDQFESPNKHAFVNGHSITFRDADDDVPRYVHGVSSEIAQMFQDYH
                     DATMPEVERRLGTLRARRVQLGNHSIFPNGILGLRLALPRGPLKTEFWHFVLLDKGAP
                     EALKRAIRVGSQANNGAAGMFEQDDVDNWRQVTAASASRLGRRYPQDLSMGLGHAGPH
                     PDYPGLVSERYISENNQRLFYQRWEEFMNAESWADIPLDPIKARFEGTATLRG"
     gene            7978..8553
                     /locus_tag="VNU03_14780"
     CDS             7978..8553
                     /locus_tag="VNU03_14780"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF007479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HWP75784.1"
                     /translation="MKKKRSRSKVRPARRPSARAATLTAQQALWLELMPFYIREAWLL
                     DERKLEEWLDLFTDDVLYFMPRRKNVPRREAHRELTPLGDLAILEEDKRYLQMRVARL
                     DTGMAWAEDPPSRTRHLVGNLEVAPLARGEVQAKTAFLVYRSHLETDHQILSGCREDV
                     LRRVKGGWKIARRTIVLDANVLLDKNLSVFL"
BASE COUNT         1292 a         3152 c         2996 g         1229 t
ORIGIN      
        1 ccgcgcaccg ccgagtcggg tccgctcccc tcgctgctgt gcttcatgta gtgcacgacg
       61 accgggaacc tggtcagcgc cggcgtcgtc tgctcggcgt tgtgctggac cttcgggtcc
      121 gcgatcgcgg cacagatgct ccgcgagtcg accaccacct ggccgagcga cggcgaaggt
      181 tcggcggcca ggaggagcgc ggccgcggcg aggcccgcga tccgaagccg ccgggacgga
      241 attttcacgt cgggacttcc cggataccgt cgaacagctc ggcggcgtgg aagacccccg
      301 gcagcgtgtc gaggtcgcgc acgaggtaat agtcctcgac cgcgtcgtac tcctcccgca
      361 ggccgaccgg ctcgccgagg gccagcttca cgtaggtgtg gggcatgttg tgcttgccca
      421 cgtggtcgaa ggcggtcatg ccgatgaaga agcgcccggc gttgatctcg gtgatgcacg
      481 gcaccccgtc gcggtcctcc ttgaggtcga cgctgaacgc gcccgacgcg gtccggctca
      541 gcgcccggat cgcccgcaca cacgtgtcga ggatccgccg gtcgatcacc gtcttggcca
      601 gcgaggagag cgacgagacc ccgctcgggc tgttctcgcc gccgaagtag gccagccgct
      661 cgaaggtgtt ggcgagcacg agggccccgc gccgccagag gctctggcag aggaagtccc
      721 ggccgggcag gtactcggac agcgtgaagg aggtggcggg caccccgcgc agctcctccc
      781 agtggctgat ccagccgcgc gcctgcgcgg gattcgtcac cggggcggca ccgagcgatc
      841 gcgtgccggt ccgcgcccgg caccagagcg gtacccgtcc gtgcagcgat tcgaagagct
      901 cctcgatccg cgagagcgcg ccgaccggat aggtatccgg cgccggcacc cctcgctgcc
      961 gcaagaaggt ggtcagccgg tacttgtcct ggcagagccg gatgatcgcc cggctcggca
     1021 ggaagagccg gcccgggaag cgctgccggc gctccgacag cgcgtccacg tccgcgtctc
     1081 cggtgggaat gatcaggtcg atccgctcgg cgtcgacgat cccccgcacc gagcggatga
     1141 acgccgcgcc gccgggtggg gccatcaagt agttccggtc cgccgcggac ttcttgacga
     1201 cgaagcgctc cgcgtgcgca ccgacgacga agagcgacgg gtcgcccgcc ttcaggctcc
     1261 ggatgaggtt gttgctggcg cccgtgcccg cgccgatgac gaggattcgt ctcctcatgt
     1321 gctctcctga cgaagggcga cggtgacggc gcggctgctc cggcagctac gccgggggga
     1381 tcgcgagggg atcgtaggtc accgaccggg acaagtcaac agccgccggg cgcctgtctg
     1441 gagccgaaca catgccgcgt gacactgtct ccgagcgatc gagagggcgg cccgcccgga
     1501 gcgcttcggg cgtttgaatg tcgctatttg agggcccttc gcgggtggca cctggcttgc
     1561 ccgtggatcc gggcggagcc gcccttgcac ccgtcacgac gcccccgcca ccgcaaccac
     1621 ccccggccgc cccgaggcgc gcgcccgggc cgcgccggcc gctatctgat cgtcaacgcc
     1681 gacgacctcg gcgccgacaa cgggaccacc cgcggcatca ttgaggctca cgagcgaggg
     1741 atcgtgacca gcgcgagcct catggtcgac atgccgggcg cgcgcgccgc ggcgcggatg
     1801 gctcgcgagc atccgcggct gggcgtcggc ctgcacgtga acttcaccgc gggcccgcgc
     1861 gcgctcgatc cgggcgacct ccgggcgcag cagcgggagc tcgaacgtca gcttgatgtc
     1921 ttcggcgacc tgaccggcgc actcccgacg cacatcgact cgcatcatca cgtgcatcgg
     1981 agggcggacg tggcgtcgct gttcgtcgag ctggcccggg atcgcggcat cccgctccgg
     2041 ggcttcggcg ggatcaccta cgtcggcaac ttctacggcc agtgggaaca cggcacgacg
     2101 gacctgcggc acatcagccc ggactatctg atcgccctcc ttcggagcat cgcgcccggc
     2161 ttctcggagc tcgcctgcca tcccggcgac cgggacgccc ggttcgatcc ggtctacgac
     2221 tggcagcggc aggtcgagct cgaatccctc acgaatccgc gggtgaccgc ggcggcggcg
     2281 caggagggaa tccggctcgt cgactaccgg gagtaccgcc ggctcgcccc cgccgcgggc
     2341 gccagcctcc cccgctactc gacgacctga cgccggtcca ccacccggac cgccttgccg
     2401 ggcggccgtg gcagcgcgcc ttgcgcgagc aacgtgatct ccgggctcag gttcagcacc
     2461 tcgcggagct cctggcggag ccgcggcagc ccggtctcgg caaagccctc gcgcgcttcg
     2521 accagcagca ccagccgatc gctgccggcg ggcgcgcgat cgagcacgat ctggtactcg
     2581 tggccgacgc cgggctgctt cagcagcagc gactcgatct gccccgggta gaagttcacg
     2641 ccgcggaaga tcaccatgtc gtcggtgcgc ccgtggagcc ggtccaggcg caggtgggtg
     2701 cggccgcagg cgcacggccc ccggctcagc acccgcgtga ggtcgcgggt gcggtagcgg
     2761 atcatcggca gcccctcgcg ggtgagcgtg ctcaccacca gctcgccctc ctcgccgtcc
     2821 gcacgaggac gtccggtcgc gggatccacc acctcgcaga agtagtgatc ctcccagacg
     2881 tggatgccct cccgcgcggc gcagtcgatg ccgagccccg ggccgccggt ctccgtcatc
     2941 ccgatgatgt cgaacgtggt caatgacagc tcgcgctcga tgagccggcg caggtcgtcg
     3001 gaccacatct cggaaccgag gatggccacc cggagcttca gcgaccccag gtcgaagcgc
     3061 tcctcgcgcg cgacctcgag gatgcgcagc gggtaggtcg agatgccggc cagcacggtg
     3121 gtcccgaggt cgcgcatgag ctggagttgc agcgacgtgc gcccggcgcc gatcggcacg
     3181 accatcgccc cgatccgctc ggccccgtag tggaagccga agccgccggt gaagaggccg
     3241 aacgacggcg tgatctggat cacgtcccgc gcgccgacgc ccgccgcggt gtagcagcgg
     3301 gccatcaccg cggcccactg ctcgacgtcg gcccgcgtga acgggttgac gaccggattg
     3361 ccggtggtgc cggaggacat gtgtacgcgc agcacgcgtc cgggcccggc gcaggccagg
     3421 ccgaacggat aggcgtcgcg cagctcgtcc ttggtgagga acggcaggag ggcccagtcg
     3481 gccgccgagc ggatgtcgcc gggctccaca cggccgaggc gccggcgcca cgcggggttg
     3541 gtcagggcgt ggccgagcgt gcggcgcatc ccctcgaccg tctcctgctc cagggcctcc
     3601 cgcggccgcg tctcggcctc cgcctcccac atcgccgcgc ccatcacgag ggccgcgccg
     3661 ccagcgcgcg gaacacgcgc tcgggcgtca tcggcagctc ggtgagccgc acgcccaccg
     3721 cgtccttcac cgcgttggcc accgcggcgg ccaccgggat gaccccggac tcgccgagcc
     3781 ccttggcgcc gaaggggccg gcctggtcga cggtctcgat caggtggatc acgatctcgg
     3841 gcatgtcggc cgcggtcagc agcgcgtact ccatgaactg cgggttcagc acccggcctt
     3901 cctcgatccg cagctcctcg gagagcgcgt acccgagccc catgtggatg ccgccgtgga
     3961 tctgcccctc ggcggccagc gggttgagcg cgcggcccac gtcgtgggcg gaggccacct
     4021 tcagcacccg cacctgcccg gtcgccaggc tcacctccac cagcgcggcc tgcgccgcga
     4081 agccgtacgc ggcggagacg ttgccgcgca ggtccttcga cagcatctcg ttgggcggat
     4141 cgtagaacgc ctccgccacc aacgtacggc cgccctcgcg gaagtggccg gcccgcacca
     4201 cgcggtcgta ctccatgccc cgctccggat gccccttcac ccggacgcgg ccggcggcca
     4261 gctcgaggtc gtccggacgc tcgtccatca ccgaggcggc cagggcgagg agcttgccct
     4321 tgaggatgcg ggccgcctgg agcgccgcgt tccccgcgat gaacgtggtg cggctcgcgt
     4381 gcacccccac gtcccacggc ttgatggacg tatcgctgtt caccacctcg atccgcccga
     4441 gcggcacgcc gagctcctcg gccacgatca tcgccagcac cgtctccgag ccctgcccga
     4501 tctcgctggc gccggtgagg agcgagacct tgccgaagtc gtcgagcttg aggatggcgc
     4561 cgcagccgtc ggagcgatag acccgcgcgc cgccgcccac gtggaacatg gccgcgtagc
     4621 cgacgccgcg tcgccagccg ggacgcgggg cctcgcccgc cgcccggctc atctcgcgct
     4681 cgaccgcgtc caggcactcg gtcatggcgc aggaggtgat gatgttgcct tgcgggttca
     4741 cgtcgccgga gcgggtggcg ttgcggcggc ggatctcgac gcggtcgagg ccgagccgct
     4801 cggccaggtc atccatctgg gcctcgatcg cgaacgtgct ctcgaggttg ccgtagcccc
     4861 gcatggaccc ggagtaggga ttgttggtgt agacgatgct cgtgtcgaac cgcaccgcgg
     4921 gcacacgata gagcccggcc gtcgtcgaga gcatcacgta gggcgtggtc gagccccagg
     4981 agacgtaggc gccgttgtcg atcaccacgc gcgcgtcccg ggccagcagc cgtccgtcgc
     5041 gatccgccgc ggtccgcagg tggatcacgc agggctcgcg ggtcgggctc gcgatgaact
     5101 cctcgatgcg ctcgaagacg atcttcaccg gccgcccggc gcggcgggcc agcagcgcgg
     5161 tgatgatgtc gatcgggtag agatcgagcc cgcggccgaa gttgccgccg accggcggct
     5221 gcatcacgcg gacccgatcg ccggtgatgc cgagcgcctc ggcgaggtcg cgctgataga
     5281 ggaacggcac ctgggtcgtg ctccacatgg tcagcgccgc gtcggggctc tccggcgggc
     5341 tccaggccgc gatcgccacc atggtgccca tgcacgcggt ggtgacgtag ttgagccggt
     5401 actcgccctc gaccaccacg tgggcccgcg cgaaggccga ctccacgtcc ccgtgggcga
     5461 actggtagcg gaggttgacc aggttgccgc cacgctcccc gtgcacgagc ggggcgcccg
     5521 gccgcagcgc cgacgccggg tcgaagaccg cgggcagctc ctcgtagtcg acccggatga
     5581 gccgcaccgc ctcctgggcg atctcctcgg tcagcgcggc caccgcggcg atctcgtccc
     5641 ggatgcagcg caccttgccg ccgtccgccg ccccgccctt gagcggcagg tggtccttgg
     5701 cgaagccgaa cgggtgctgc tccacgcggt cggcggtgat caccgcgacc accccgggca
     5761 gcgcctcggc cgcgctcgtg tcgatgcgcc gcacgcgcgc gtgcgggtgg cgggtgcgga
     5821 gaatggcccc gtgggccagg cgtggcaggg tgaggtcgtg gaggaactgg gtgtggccgg
     5881 tgaccttctc ggccccgtcg cgcttcgggg tgtccttgcc gatgacgctg aagctcaccg
     5941 ccgtcgcgct ccggagacga tcgcggccgg gctaccgggc cgtggccatc cgcgtcacct
     6001 gcgccgcccg ccactcggcg cgggacatga acccgggacg gttgcggcag agcgcctccg
     6061 tctcccgctc gatggtctcc accggcgcct cgatgtcgaa gggctcgacc accggctgcg
     6121 gggtgtgcca tggcgtgtcg acgaagacct cgagccggtt gccctccgga tcggcgaagt
     6181 agaccgacca cgcgttgccg tgggtcacga cgcggaactg cttgatgcct tcctcgcgcg
     6241 cccgcgcgtg catcgccttg aggtcgacga ggcccggcac cttgaacgac atctggttca
     6301 ccacgttgaa ctcgactccg ggcggccggc cggtggccag cacgatctgg tggtgctcgt
     6361 ccgggtcgcg ggagagaaag accagattcg acccgcccgg cacctgtccg cgatcgctca
     6421 cgaggagccc gagcacgcgg gtgtagaagt cctccatccc cgcgaggtcg gtcacgtaga
     6481 tcccgaagtg gctgaacgag acgggcggcg atgtggtcat gagggctgcc tccgcgcgag
     6541 tggtgtcctg tcgcggatga tagcacgccg aaaccccggt gaagatccgc ggcgggttcg
     6601 gcctataatg agcgcgacaa ggaggagcgc ggcatgggcg ccacccagag gtacgtcgtg
     6661 atcgaccccg ccaccggcaa gatcgaccgc cgcatcttca gcgaccaggc ggtctacgac
     6721 gacgagatgg agaggatctt cgggcgcgcc tggctcatga tcgggcacga gagcctggtg
     6781 cccgcggccg acgacttctt ccacacctac atgggcgagg acccggtcat cctgacccgc
     6841 gacgcccagg gccggctcca cgcgctgctc aacatgtgcc ggcaccgcgg caaccgggtg
     6901 gtgcgctgcg acgacggcaa cgccaaccgc ttcatgtgca cctaccacgg gtggacctac
     6961 aagaacgacg gcacgctcga tcacgtcccg ggcaaggccg aggcctacta cgacgcgctc
     7021 gaccgggact cgctcggcct ggtccaggcc cgcgtggaga cctacgccgg gatcgtgttc
     7081 gcgacgtggg ccaaggacgc ccccagcctc gaggcctacc tgggtgacgc ccgctggtac
     7141 ctcgacaccg tgttcaaccg gcgcgacggc ggcatgcagg ccctgggccc gatgaagtgg
     7201 ctcgagccgg tcaactggaa gacgatggtg gacaactgct cggacaacta ccacgtgccg
     7261 accactcacc tctccagcgc ccgcgtgcag accaagttcc tcgggcgccc ccggctctcg
     7321 cacaaggacc agttcgagag cccgaacaag cacgccttcg tcaacggcca ctcgatcacc
     7381 ttccgcgacg cggacgatga cgtcccccgc tacgtgcacg gcgtctcgag cgagatcgcc
     7441 cagatgttcc aggactacca cgacgccacg atgcccgagg tcgagcggcg gctcggcacg
     7501 ctccgcgcgc gccgcgtgca actcggcaac cactcgatct tccccaacgg gatcctcggc
     7561 ctccgcctcg ccctgccccg gggtccgctc aagaccgagt tctggcactt cgtgctgctg
     7621 gacaaggggg cgccggaggc cctcaagcgg gcgatccgcg tcgggagcca ggccaacaac
     7681 ggcgccgccg gcatgttcga gcaggacgac gtcgacaact ggcgccaggt gaccgccgcg
     7741 agcgcgagcc gcctcggccg ccggtaccct caagacctgt cgatgggcct cggccacgcg
     7801 gggccgcatc cggactaccc gggactcgtc tccgagcgct acatctcgga gaacaaccag
     7861 cgcctcttct atcagcgctg ggaggagttc atgaacgcgg agagctgggc cgacatcccg
     7921 ctcgacccga tcaaggcccg cttcgagggc accgcgacat tgagaggcta gcggcctctg
     7981 aagaagaagc gctcgcgctc caaggtccgc cccgcacgac ggccctccgc gcgcgcggcc
     8041 acgctcaccg cccagcaggc cctctggctc gagctgatgc cgttctatat ccgcgaggcg
     8101 tggctgctcg acgagcggaa gctcgaggag tggctcgacc tcttcaccga cgacgtcctc
     8161 tacttcatgc cccgccgcaa gaacgtcccg cgccgggagg cccaccgcga gctgacgccg
     8221 ctcggcgacc tggcgatcct cgaagaggac aagcgctacc tccagatgcg ggtggcccgc
     8281 ctcgacaccg ggatggcctg ggccgaggat ccgccgtcgc gcacccgcca cctggtcggc
     8341 aacctcgagg tggcgccgct ggcccggggc gaagtgcagg ccaaaactgc gttcctggtc
     8401 taccgctcgc atctggagac cgaccaccag atcctctccg gctgtcgcga ggacgtgctg
     8461 cgccgggtga agggcgggtg gaagatcgcc cggcgcacga tcgtgctcga cgccaacgtc
     8521 ctgctcgaca agaacctcag cgtcttcctc taggccgtcc gtcggatggg atcgcagccc
     8581 cgatgacggc gaccatgatg gtcgcgctgg tcgccggcac gtgtctgggc ggcttcgtgc
     8641 agggcgtatc cggcttcgcg ttcgggctg
//
LOCUS       DATMCV010000643        11017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_697498, whole genome shotgun sequence.
ACCESSION   DATMCV010000643 DATMCV010000000
VERSION     DATMCV010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11017
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697498"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VNU03_14785"
     CDS             complement(<1..122)
                     /locus_tag="VNU03_14785"
                     /inference="COORDINATES: protein motif:HMM:NF014930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75785.1"
                     /translation="MTDPARYETIIGLEVHAQLSTVTKMFCGCRAVFGAPPNTAT"
     gene            complement(119..1585)
                     /gene="gatA"
                     /locus_tag="VNU03_14790"
     CDS             complement(119..1585)
                     /gene="gatA"
                     /locus_tag="VNU03_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HWP75786.1"
                     /translation="MAALASLTAHELGARYRSGEATPTQAATEYLARIEALDPEVRAF
                     LTVTREDALRRAAAADARFKAGTPLGPLDGVPVALKDVLCTRGIRTTCGSKILERFVP
                     PYDATVVARLFEGGAVLLGKLNMDEFAMGSSTEHSAFFTTHNPWDLARVPGGSSGGAA
                     AAVAAGMAALTLGTDTGGSIRQPAAFSGVLGMKPTYGRVSRYGVIAFASSLDQVGPFA
                     RDAEDAALMLGVIAGADPMDATAIDVPVPDYRAALGQGIEGLRLGVPAEYFMDGMDPE
                     VEQAVRTAIATLEKLGAHTEPVSLPHTEYGLAAYYVIAPAEASSNLARYDGVKYGLRA
                     PGARDLIDMYSKTRAAGFGAEVKRRVMLGTYVLSAGYYDAYYGQAQKVRTLVRRDFEQ
                     AFSRVDLLVAPTTPGVAFKMGEREDPLQMYLNDIFTIPVNLAGLPGVSIPGGFTQAGL
                     PIGLQLIAPAFDEATLLRAAHAYQQVTDWHTRKPPLST"
     gene            complement(1586..1891)
                     /gene="gatC"
                     /locus_tag="VNU03_14795"
     CDS             complement(1586..1891)
                     /gene="gatC"
                     /locus_tag="VNU03_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWP75787.1"
                     /translation="MATPKTKISIEEVDHVARLARLDLSAADKERMRSELDKILGYID
                     KLRALDTRDVEPTSHAVPVTNVMRDDVERPSLPVEDMLANAPDRYREMFRVPRIIEE"
     gene            complement(1989..4154)
                     /locus_tag="VNU03_14800"
     CDS             complement(1989..4154)
                     /locus_tag="VNU03_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWP75788.1"
                     /translation="MTAESLLAPLNPPQRDAVTTTKGPVLVLAGAGSGKTRVIAHRIA
                     YLLAVEGIGPRHVLAVTFTNKAAAEMRHRVEDLLLPAGIRPPLIATFHSACVRILRER
                     AVHAGLPPSFVIYDEDDRQALVKEALRELDFDERTLTPSTVVHRISHCKNQMLTPADV
                     EQLARSPREERIASLYRLYEQKLQAAGALDFDDLLLRVVRLLETVPEALRWYRTVWTH
                     VLVDEYQDTNRAQYRIVQLLTEEHRNLCVVGDPDQSVYRWRGADLRNILDFEKDYPDC
                     RVVALEQNYRSTKRILGIASAVIVNNTARRDKRLWTENHEGERPEVYRAWDENEESAF
                     VAQKVRALRGEGRDFRDMAVFYRTNAQSRVLEDALRRAGVPYLIVGGVRFYERREIRD
                     VVAYLRLVSNPLDDVAFRRAVAAPARGIGKATLDRLADGARAAGVPLLALCAAIPADI
                     TGKPRRALEEFAGVIARLGERRGGLTVPALIDEVCAASGYREALKAERTAEAQARLEN
                     LEELVAASEEFIVTQQAAGLETVTLEAFLDSIALVADTDEIDPEAGGVTLMTLHSAKG
                     LEFPVVFLTGLEEGVFPHSRSMLEPEELEEERRLCYVGITRARERLLLSYALHRRIQG
                     YGVAEPSRFLLEIPEEQLHLINARRPDPVAPAVSATAAADPGDDFPFRLGAKLRHARW
                     GEGLLVGMEREGADVIATVHFASVGRKRLSLQYAHLEEL"
     gene            complement(4245..6083)
                     /gene="glmS"
                     /locus_tag="VNU03_14805"
     CDS             complement(4245..6083)
                     /gene="glmS"
                     /locus_tag="VNU03_14805"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWP75789.1"
                     /translation="MCGIVGYVGDKSAIDIVIEGLKRLEYRGYDSAGAAVIGPEGLQI
                     RRAAGRIKILEGILRERPIDGPIGLGHTRWATHGRPTDENAHPHTDGSGELVVVHNGI
                     IENYLPIKERLQAEGHVFKSETDTEVIAHLIERYLKDVPRLDEAVRRALRELRGSYAI
                     VVLHRRMPDRLVAAKLGAGSVVVGLGENETFLASDIPAILSHTRDVVVLEDGDVAIIT
                     RAGVEITDLDGAPVLRTPSRILWDPIMAEKGGYRHFMLKEIFEQPRAAADTMRGRVQL
                     EGGTVVLPDVQLDPDVVAGIQRVILVACGTSYHAAIVGRFMMERLTGIASEVDIGSEF
                     RYRDAVVGPETLIVALSQSGETADTLGAVKAARAKGAPIVGITNVVGSALSREATGTL
                     YTHAGPEIGVASSKTFTATLVACYLLALWLGRRRGALLAEDGRKHIQGLLELPRLMEQ
                     TLEMENAVADLAREVSRHKNFLFLGRGINYPLALEGALKLKELSYVHAEGYPAGEMKH
                     GPIALLEDGFPVIALNPRDSSYDRMMGNLEEVRAREGRVISIGHEGDRELRAKSDIML
                     TVPPAGDLLTPMLLSIPLQLLAYHMAVRLGRDVDQPRNLAKSVTVE"
     gene            complement(6088..7482)
                     /gene="glmU"
                     /locus_tag="VNU03_14810"
     CDS             complement(6088..7482)
                     /gene="glmU"
                     /locus_tag="VNU03_14810"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWP75790.1"
                     /translation="MNQLTAIILAAGEAKRMRSARPKVLHALCGRPLIAYPVGAARAV
                     GARVVVVVGRAAEEVRASVAPEAAATFVEQKERLGTGHAVLQTHVACGDAAGTVLVLP
                     GDVPLLSEATLRRLVEHHVASHAAATLLTAEVADPTGYGRVVREQGRPIGIVEHRDAT
                     AAQREIREIGTSVYCFDARRFWPALAQVTPDNAQHEYYLTDVIGILHRQGERLEAVIT
                     DDPSECLGVNDRRQLAELGGIMRHRILDRLMAAGVTVIDPATTYVDDTVTIGMDTVLH
                     PNVSLTGRTVVGAECVVSAGCQVTDTTIADGVLLKPYCVLAESTVETGAQLGPFCHLR
                     PLSHVGANAKIGNFVELKKSKIGRGSKVPHLSYVGDTQMGEGVNVGAGTITCNYDGAK
                     KHETVIGDGAFVGTNSSLVAPLTIGEGAYVAAGSVITKNVPPGSLAVARGRQETREGW
                     VSRRHKDKKAKHGE"
     gene            complement(7595..8572)
                     /locus_tag="VNU03_14815"
     CDS             complement(7595..8572)
                     /locus_tag="VNU03_14815"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWP75791.1"
                     /translation="MTGDRRVTHVVEGDGAGARLDQWLARRLPELSRMRVQALITTGR
                     ALVDGRAVKAAHRVQAGERVEVAIPPPPRDGLEPEAVPLRVVFEDAHVLVVDKPAGMV
                     THPGAGRIDGTLAAAALAHAPEMAGVGSARRPGIVHRLDKGTSGLIVLAKTQAAYDGL
                     TAQLARRTVSRRYLALAQGALRRADGVVDAPIGRDPRSRIRMAIVPEGRGKRAVTHYR
                     VLERFGSGASAVTLLECRLETGRTHQIRVHVASLGHPLLGDDTYRGRRAAPAGDPVLA
                     DLVSELGGVALHAAGLAFTHPVTGAAMSFACPSPDRIERIVSHLRNRAG"
     gene            complement(8595..9068)
                     /gene="lspA"
                     /locus_tag="VNU03_14820"
     CDS             complement(8595..9068)
                     /gene="lspA"
                     /locus_tag="VNU03_14820"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWP75792.1"
                     /translation="MTSAALTALVVLILDQLTKVWALQGLAPGRPVPVVDGFFSLTLV
                     MNPGLAFGMLSSTPAGWRWVVALLSIGALAVLAVVGLRMLPGGGRLTPLALGLIFGGA
                     VGNLIDRGRFGAVVDFLDFYWRGYHWPAFNAADSAITVGVGLLALRLLTAPPSTS"
     gene            complement(9065..>11017)
                     /locus_tag="VNU03_14825"
     CDS             complement(9065..>11017)
                     /locus_tag="VNU03_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWP75793.1"
                     /translation="AVPGRALEGLVARHPWIDRDSPVVTADFVAMDAGTGLVHIAPGH
                     GEEDYEVGRRAGLKIYNPVDDDGRFIPEVAHFAGLTVWEANPRIIEHLRSLGALVAEV
                     SLQHTYPHCWRCKNPTLFRATEQWFIALDRHGLRQKALDAIRNEVRWIPGWGEERIYN
                     MVAHRPEWVISRQRVWGVPIVAFYCTGCDALLLDEALVEHVAAIMRTGAGADEWYMRE
                     AVDLLPPGTRCPKCGGSGFRKETDILDVWFDSGCSHAAVLEQRPELRWPAEMYLEGSD
                     QHRGWFHSSLLEAVGTRDRPPYRSVLTHGFVVDGDGRKMSKSGGNNISPDELIPKYGA
                     EVLRLWVAAEDSSEDIRLSLEILNRLADAYRRIRNTSRFLLGTLADFDPERDRRSYDQ
                     MDELDRWALLRLGDLISRVRKAYDDYQFHVAFHALHNFCAVALSALYLDTIKDRLYTS
                     APDDPRRRASQTVCFELLTALTRLMAPVLTFTADEVWSYIPGRGKPESVHLVLFPEER
                     GEWTNERLAADWERLLEVRGEVSRALESARQQGKIGKGIDAVVFIASAPEEQWRPLLE
                     AKGEALLTTLFNVSGTRLGKPAPAGSVRYESQDIPGLVLEVVPAQALGWKKCERCWTW
                     SARVGEHPAHPTLCERCTPVVTALAR"
BASE COUNT         1541 a         3864 c         4036 g         1576 t
ORIGIN      
        1 gtggcggtgt tgggcggggc accgaacacc gcccggcagc cgcagaacat cttggtcacg
       61 gtcgacagct gagcgtgcac ttcgaggccg atgatggtct cgtagcgggc ggggtcggtc
      121 atgtcgagag cgggggtttg cgggtgtgcc agtcggtgac ctgctggtag gcgtgggccg
      181 cgcgtagcag ggtggcctcg tcgaaggcgg gcgcgatgag ctgcagcccg atcggcagcc
      241 cggcctgagt gaagccgccc gggatcgaca cccccggcag cccggccagg ttcaccggga
      301 tcgtgaagat gtcgttcagg tacatctgga gcgggtcttc ccgctcgccc atcttgaacg
      361 cgacccccgg agtggtgggc gccacgagca ggtcgacccg cgagaaggcc tgctcgaagt
      421 cccgccgcac cagcgtacgc accttctggg cctggccgta gtaggcgtcg tagtagccgg
      481 cggagaggac gtaggtcccc agcatcaccc gccgcttcac ctcggcgccg aatccggccg
      541 cccgcgtctt gctgtacatg tcgatcaggt cgcgcgcgcc gggagcccgc agcccgtact
      601 tcacgccgtc gtagcgcgcg aggttggacg aggcctcggc gggcgcgatg acgtagtagg
      661 cggccagccc gtactcggtg tgaggcagcg acaccggctc ggtgtgcgcg cccagcttct
      721 cgagcgtcgc gatggcggtg cgcaccgcct gctcgacctc ggggtccatg ccgtccatga
      781 agtactcggc gggcaccccg aggcgcagcc cctcgatccc ctgcccgagc gcggcccggt
      841 agtcgggcac cgggacgtcg atcgcggtgg cgtccatcgg gtcggcgccc gcaatgaccc
      901 cgagcatcag ggcggcgtcc tccgcgtccc gggcgaacgg gccgacctgg tcgagcgacg
      961 aggcgaacgc gatgacgccg tagcgcgaca cgcgaccgta cgtgggcttc atcccgagca
     1021 cgcccgagaa ggcggcgggc tggcggatcg agccgccggt gtcggtcccc agcgtgaggg
     1081 cggccatgcc ggcggccacc gcggcggcgg cgcctccgga ggacccgccc ggcacgcggg
     1141 ccaggtccca cgggttgtgg gtggtgaaga acgcggagtg ctcggtggaa gagcccatcg
     1201 cgaactcgtc catgttgagc ttgccgagca gcaccgcgcc tccctcgaag agccgcgcga
     1261 ccaccgtcgc gtcgtagggc ggcacgaacc gctccaggat cttcgagccg caggtggtgc
     1321 ggatgccccg ggtgcagagc acgtccttga gcgcgaccgg caccccgtcg agcgggccga
     1381 gcggggtccc ggccttgaag cgggcgtcgg ccgccgcggc gcgccggagg gcgtcctcgc
     1441 gggtcacggt gagaaaggcg cgaacctcgg gatcgagggc ttcgatgcgc gccaggtact
     1501 cggtcgccgc ctgggtcggg gtggcctccc cgctccggta gcgggcaccc agctcgtgag
     1561 cggtcagcga cgccagcgcg gccatctact cctcgatgat ccgggggacg cggaacatct
     1621 cgcgatagcg atcgggcgcg ttggcgagca tgtcctccac gggcagcgac gggcgctcga
     1681 cgtcgtcccg catcacgttc gtgaccggga ccgcgtggga cgtcggctcc acgtcgcggg
     1741 tgtcgagcgc gcgcagcttg tcgatgtagc cgaggatctt gtccagctcg ctccgcatcc
     1801 gctccttgtc ggcggcggac agatcgaggc gggcgaggcg ggccacgtgg tcgacttctt
     1861 cgatggagat tttcgttttg ggtgtggcca ccgttagatt ctcttcatct tcaacatggc
     1921 tgagccatgt tgaccgtggg cgagatggtc gcggcgaccc gcggttgtca cggagggctg
     1981 gcggcgagtc atagctcctc gaggtgggcg tactgcagcg agaggcgctt gcggccgacg
     2041 ctggcgaagt ggacggtggc gatcacgtcg gcgccctcgc gctccatgcc caccagcagg
     2101 ccctcgcccc agcgcgcgtg ccggagcttg gcgcccaggc ggaacgggaa gtcgtcgccg
     2161 ggatcggccg cggccgtcgc ggacaccgcg ggggccaccg gatccggccg acgcgcgttg
     2221 atcaggtgga gctgctcctc cgggatctcg aggaggaagc gggacggctc ggcgaccccg
     2281 tagccctgga tgcggcggtg gagcgcgtac gagagcagca gccgctcgcg ggcccgcgtg
     2341 atgcccacgt agcagaggcg ccgctcctcc tccagctcct ccggctccag catggagcgc
     2401 gagtggggga agacgccttc ctcgagcccg gtgaggaaga ccaccgggaa ctcgagcccc
     2461 ttggccgagt ggagggtcat gagcgtcacc ccgcccgcct ccggatcgat ctcgtcggtg
     2521 tcggccacca gcgcgatcga gtcgaggaag gcctcgaggg tcaccgtctc gagcccggcc
     2581 gcctgctggg taacgatgaa ctcctccgac gcggccacca gctcctccag gttctcgagc
     2641 cgggcctggg cctcggcggt gcgttccgcc ttgagcgcct cgcgataacc ggaggccgcg
     2701 cacacctcgt cgatgagggc gggcacggtc aggccgcccc ggcgctcgcc caggcgggcg
     2761 atcacccccg cgaactcctc cagcgcccgg cgcggcttgc cggtgatgtc ggcgggaatc
     2821 gcagcgcaga gcgcgagcag cggcactccc gccgcgcgcg cgccgtcggc caggcggtcg
     2881 agggtggcct tgccgatccc gcgcgcgggc gcggccaccg cgcgtcggaa cgcgacgtcg
     2941 tcgagcgggt tcgagaccag ccggaggtag gcgaccacgt cccggatctc gcggcgctcg
     3001 tagaagcgga cgccgcccac gatcaggtac ggcacgccgg cgcggcgcag cgcgtcctcg
     3061 agcacgcggg actgggcgtt ggtgcggtag aacaccgcca tgtcgcggaa gtcgcgcccc
     3121 tccccgcgga gggcgcgcac cttctgggcg acgaaggcgg actcctcgtt ctcgtcccac
     3181 gcgcggtaga cctcgggccg ctcgccctcg tggttctcgg tccacagccg cttgtcccgg
     3241 cgcgcggtgt tgttcacgat gaccgcggac gcgatgccga ggatgcgctt ggtcgaccgg
     3301 tagttctgct cgagcgccac cacccggcag tcgggatagt ccttctcgaa gtcgaggatg
     3361 ttgcgcagat cggccccgcg ccaccggtag accgactggt cgggatcgcc caccacgcag
     3421 aggttccggt gctcctcggt caggagctgc acgatgcggt actgcgcgcg gttggtgtcc
     3481 tggtactcgt ccaccagcac gtgggtccac accgtgcggt accagcggag cgcctccggc
     3541 accgtctcga gcaggcgcac cacccggagg aggaggtcgt cgaagtcgag cgcgccggcg
     3601 gcctggagct tctgctcgta gagccggtag agcgacgcga tgcgctcctc gcgcgggctg
     3661 cgggcgagct gctccacgtc ggccggggtc agcatctggt tcttgcagtg gctgatccgg
     3721 tgcacgaccg tgctcggggt gagcgtccgc tcgtcgaagt cgagctcgcg cagcgcctcc
     3781 ttcacgaggg cctgccggtc gtcctcgtcg tagatcacga aggacggcgg cagccccgcg
     3841 tggaccgcgc gctccctcag gatgcgcacg caggcggagt ggaaggtagc gatgagcgga
     3901 ggccggatgc ccgcgggcag cagcaggtcc tccacccggt gccgcatctc ggcggccgcc
     3961 ttgttggtga aggtcaccgc caggacgtgc cgcgggccga tgccctcgac cgcgaggaga
     4021 taggcgatgc ggtgggcgat cacccgggtc ttgccgctgc ccgccccggc cagcaccagc
     4081 accgggccct tcgtcgtcgt gaccgcgtcg cgctgcggag ggttgagcgg cgcgagcaga
     4141 ctctcggcgg tcactgcggg gtgcggcatg gagcctaacg ttaccacaac cgcgaggggt
     4201 cgtggccctc accctgccct ctccccgggg ggagagggtg aaagctactc gacggtgacc
     4261 gacttggcca ggttccgcgg ctggtccacg tcgcggccga gacgcaccgc catgtggtac
     4321 gcgaggagct gcagcgggat cgacagcagc atcggggtga gcaggtcgcc ggccggcggc
     4381 accgtcagca tgatgtcgga cttggcccgc agctcgcggt ccccctcgtg gccgatggag
     4441 atgacccgtc cctcgcgggc gcgcacctcc tccaggttgc ccatcatgcg gtcgtaggac
     4501 gagtcgcgcg ggttgagcgc gatgaccggg aaaccgtcct ccaggagcgc gatcggcccg
     4561 tgcttcatct ccccggccgg gtagccctcc gcgtgcacgt aggagagctc cttcagcttc
     4621 agcgcaccct cgagggcgag cgggtagttg atcccgcggc ccaggaagag gaagttcttg
     4681 tgacgcgaca cctcgcgggc caggtccgcc accgcgttct ccatctcgag cgtctgctcc
     4741 atgagtcgcg gcagctcgag caggccctgg atgtgcttgc gcccgtcctc ggccagcagc
     4801 gcgccccggc gacggcccag ccagagggcg aggagatagc aggccaccag ggtggcggtg
     4861 aaggtcttgg aggaggcgac cccgatctcg gggccggcgt gggtgtagag ggtgccggtg
     4921 gcctcgcgcg acagcgcgga gccgaccacg ttggtgatgc ccacgatcgg ggcgcccttg
     4981 gcccgggcgg ccttgaccgc gcccagggtg tccgcggtct ctccggactg cgagagcgcg
     5041 acgatgaggg tctccgggcc caccaccgcg tcgcggtagc ggaactcgga cccgatgtcg
     5101 acctcggagg cgatcccggt caggcgctcc atcatgaagc gcccgacgat ggccgcatga
     5161 taggaggtgc cgcacgcgac caggatgacc cgttgaatgc ccgcgaccac gtcgggatcg
     5221 agctgcacgt cgggcagcac caccgtgccc ccttcgagct ggacccgccc ccgcatggtg
     5281 tcggcggcgg cgcgcggctg ctcgaagatt tccttcaaca tgaaatggcg gtagcccccc
     5341 ttctcggcca tgatcgggtc ccacaggatg cggctcggcg tccgcagcac cggggcgccg
     5401 tcgaggtcgg tgatctcgac cccggcgcgg gtgatgatcg caacgtcccc gtcctccagc
     5461 accaccacgt cgcgggtgtg ggagaggatg gccgggatgt cggaggcgag gaaggtctcg
     5521 ttctcgccga gccccaccac cacgctgccc gcgccgagct tggccgccac caggcgatcc
     5581 ggcatccggc ggtggagcac cacgatcgcg taggagccgc gcagctcgcg gagggcgcgg
     5641 cggaccgcct cgtccaggcg cgggacgtcc ttgaggtacc gctcgatcag gtgggcgatg
     5701 acctcggtgt cggtctccga cttgaagacg tgcccctcgg cctgcaggcg ctccttgatc
     5761 ggcaggtagt tctcgatgat gccgttgtgc accacgacca gctcgcccga gccgtcggtg
     5821 tgggggtgcg cgttctcgtc ggtggggcgg ccgtgggtgg cccagcgggt gtggccgagg
     5881 ccgatggggc catcgatcgg gcgctctcgg agaatgccct ccagtatctt gatgcgcccg
     5941 gcggcccgcc ggatctggag cccctcgggc ccgatcaccg ccgcgcccgc cgagtcgtac
     6001 ccgcggtact cgaggcgctt caggccctcg atcacgatgt cgatcgcgct cttgtccccg
     6061 acgtagccga cgattccaca catgggccta ctccccgtgc ttggccttct tgtccttgtg
     6121 cctgcgcgag acccatccct cgcgcgtctc ctggcgcccg cgggccaccg ccagcgagcc
     6181 cggcggcacg ttcttggtga tgacggagcc ggccgccacg taggcgccct ccccgatggt
     6241 gagcggggcc accaggctcg agttcgtccc cacgaacgcc ccgtctccga tgacggtctc
     6301 gtgcttcttc gcgccgtcgt agttgcaggt gatggtgccc gcgcccacgt tgaccccttc
     6361 gcccatctgg gtgtcgccca cgtaggagag atggggcacc ttcgagccgc ggccgatctt
     6421 cgacttcttg agctcgacga agttgccgat cttggcgttg gcccccacgt ggctcagcgg
     6481 ccgcaggtgg cagaagggac cgagctgggc gcccgtctcg acggtcgact ccgcgagcac
     6541 gcagtacggc ttgagcagca ccccgtcggc gatcgtggtg tcggtcacct ggcagcccgc
     6601 gctcaccacg cactcggccc ccaccacggt gcggccggtc aggctcacgt tcgggtgcag
     6661 cacggtgtcc atcccgatcg tcaccgtgtc gtcgacgtag gtggtggccg gatcgatcac
     6721 ggtcacgccc gcggccatca gccggtcgag gatgcgatgg cgcatgatcc cgcccagctc
     6781 ggccagctgg cgccggtcgt tcacgcccag gcactcgctc gggtcgtcgg tgatcaccgc
     6841 ctccaggcgc tcgccctggc ggtgcaggat gccgatcacg tcggtgaggt agtactcgtg
     6901 ctgcgcgttg tcgggcgtca cctgggccag cgcgggccag aagcgccggg cgtcgaagca
     6961 gtagacgctg gtgccgatct cccggatctc ccgctgcgcc gccgtcgcgt cgcggtgctc
     7021 gacgatcccg atcggccggc cctgctcccg caccacgcgg ccatagccgg tgggatccgc
     7081 gacctccgcg gtgaggagag tcgcggccgc gtggctcgcc acgtggtgct cgaccagccg
     7141 acgcagcgtg gcctcggaca gcagtggcac gtctcccggc aggaccaaga cggtgccggc
     7201 ggcgtcgccg caggccacgt gggtctgcag gacggcgtgc ccggtgccca ggcgctcctt
     7261 ctgctcgacg aaggtggcgg cggcctcggg cgcgacggac gcccggacct cctcagccgc
     7321 ccgccccacc acgaccacca cccgggcccc aacggcccgc gcggcaccga cgggataggc
     7381 gatcagcgga cggccgcaga gtgcgtgaag gaccttgggc cgggccgagc gcatgcgctt
     7441 ggcctctccg gcggcgagaa tgatggcggt gagctgattc atggtatcga ccatgttagc
     7501 acgcgacatg ccacgcgccg ggggcgcgcg gcggccggga atggcccgtt ctgacgcggt
     7561 gttggctacc ccccgcggtg ggccggtcgg ccgctcagcc ggccctgttg cggagatgag
     7621 acacgatccg ctcgatcctg tcgggcgaag gacacgcgaa gctcatcgcc gcgcccgtga
     7681 ccggatgcgt gaaggccagg ccggccgcat ggagcgcgac cccgccgagc tccgagacga
     7741 gatcggcgag caccgggtcg cccgccggcg cggcgcgacg gccccggtag gtgtcgtcgc
     7801 ccaggagggg atgaccgagc gaggccacgt ggacccggat ctggtgggtc cgaccggtct
     7861 cgagccggca ctcgagcagg gtcacggcgg aggcgccgct gccgaagcgc tcgagcaccc
     7921 ggtagtgggt gaccgcccgc ttgccgcgcc cctcgggcac gatcgccatg cgaatccggg
     7981 agcgcgggtc gcggccgatc ggcgcgtcga ccacgccgtc ggcccggcgc agcgccccct
     8041 gggcgagcgc caggtagcgc cggctcaccg tccgccgggc gagctgcgcg gtgagcccgt
     8101 cgtacgcggc ctgggtcttg gccagcacga tcagccccga ggtgcccttg tccaggcgat
     8161 ggacgatccc gggacggcgg gcgctgccca cgcccgccat ctccggggcg tgggccagcg
     8221 ccgcggcggc cagggttccg tcgatccgcc ccgcgccggg atgggtcacc atgccggcgg
     8281 gcttgtccac caccaggacg tgggcgtcct cgaagacgac gcgaaggggg accgcttcgg
     8341 gttcgaggcc atcccgggga ggcggcggga tggccacctc gacgcgctcg cccgcctgga
     8401 cccggtgcgc ggccttgacg gcgcggccgt ccaccagcgc gcggcccgtt gtgatgaggg
     8461 cctggacgcg catgcgcgac agctcgggaa gccgtcgggc gagccactga tcgaggcgcg
     8521 ccccggcgcc gtcgccctcc accacgtggg tgacgcgacg atcgccggtc acggcgcggc
     8581 cgccaccccg cgcctcagct ggtcgacggg ggcgcggtga gcaggcgcag ggcgagcagc
     8641 cccacgccga cggtgatcgc ggagtcggcc gcgttgaacg cgggccagtg gtaaccgcgc
     8701 cagtagaagt cgaggaagtc gaccaccgcc ccgaagcggc cccgatcgat caggttgccg
     8761 accgcgcctc cgaagatgag cccgagcgcg agcggggtga ggcggccgcc acccggcagc
     8821 atgcgaagcc ccaccaccgc cagcaccgcg agggcgccga tggagagcag ggcgaccacc
     8881 catcgccagc ccgccggcgt gctcgagagc atgccgaagg cgaggcccgg attcatcacg
     8941 agggtgaggg agaagaagcc gtcgaccacc ggcacgggcc ggcccggggc gaggccctgg
     9001 agcgcccaga ccttcgtcag ctggtccagg atcagaacga cgagcgcggt gagggcggcc
     9061 gacgtcaccg ggcgagcgcc gtcacgaccg gcgtgcagcg ctcgcagagc gtcggatgcg
     9121 ccggatgctc ccccacccgc gcgctccagg tccagcagcg ctcgcacttc ttccagccga
     9181 gcgcctgggc cggcaccact tccagcacga ggccggggat gtcctggctc tcgtagcgga
     9241 cggagccggc gggcgcgggc ttgccgagcc gcgtccccga gacgttgaag agcgtggtga
     9301 gcagggcctc gcccttggcc tcgagcagcg ggcgccactg ctcctcgggc gcgctcgcga
     9361 tgaacaccac cgcgtcgatg cccttgccga tcttgccctg ctggcgggcc gactcgagcg
     9421 cgcgcgagac ctcgccgcgc acctcgagca gccgctccca gtccgccgcg agccgctcgt
     9481 tcgtccactc cccgcgctcc tccgggaaga ggacgaggtg cacgctctcc ggcttgccgc
     9541 gtcctgggat gtaggaccac acctcgtccg cggtgaaggt gagcaccggg gccatcaggc
     9601 gcgtcagggc ggtgagcagc tcgaagcaca ccgtctggga cgcgcggcgg cgcgggtcgt
     9661 cgggagccga ggtgtagagc cggtccttga tggtgtcgag gtagagcgcg gagagggcga
     9721 ccgcgcagaa gttgtggagc gcgtggaagg ccacgtggaa ctggtagtcg tcgtacgcct
     9781 tgcggacgcg cgagatgagg tcgcccaggc gcaggagcgc ccagcggtcg agctcgtcca
     9841 tctggtcgta cgagcgtcgg tcgcgctcgg ggtcgaagtc ggccagggtg ccgagcagga
     9901 accggctggt gttgcggatg cgccggtagg cgtcggcgag ccggttgagg atctcgagcg
     9961 agaggcggat gtcctccgac gagtcctccg cggccaccca gagacgcagg acctccgccc
    10021 cgtacttggg gatcagctcg tcgggggaga tgttgttgcc gcccgacttc gacatcttgc
    10081 ggccgtcgcc gtccaccacg aagccgtggg tgaggacgga gcggtagggc ggccggtcgc
    10141 gcgtgcccac cgcctccagc agcgaggagt ggaaccagcc gcgatgctgg tcggacccct
    10201 cgaggtacat ctcggcgggc cagcgcagct cggggcgctg ctcgagcacc gccgcgtggc
    10261 tgcagccgga gtcgaaccac acgtcgagga tgtcggtctc cttgcggaag ccgctcccgc
    10321 cgcacttcgg acaccgcgtg cccgggggca gcaggtcgac cgcctcgcgc atgtaccact
    10381 cgtcggcgcc cgctccggtg cgcatgatcg cggccacgtg ctcgaccagg gcctcgtcga
    10441 gcagcagcgc gtcgcagccg gtgcagtaga aggcgacgat cggcacgccc cacacccgct
    10501 ggcgggagat gacccattcc ggccggtgcg cgaccatgtt gtagatgcgc tcctcgcccc
    10561 atccggggat ccagcgcacc tcgttgcgga tcgcgtcgag cgccttctgc cgcagcccgt
    10621 ggcggtcgag cgcgatgaac cactgctcgg tggcgcggaa cagggtgggg ttcttgcacc
    10681 gccagcagtg cggataggtg tgctggagcg acacctccgc caccagcgcc ccgagcgagc
    10741 ggaggtgctc gatgatgcgc gggttcgcct cccacaccgt gagccccgcg aagtgcgcga
    10801 cctcggggat gaagcgcccg tcgtcgtcga ccgggttgta gatcttgagc ccggcgcggc
    10861 ggccgacctc atagtcctct tctccgtggc ccggcgcgat gtgcacgagc ccggtgccgg
    10921 cgtccatcgc cacgaagtcc gcggtgacca ccggcgagtc ccggtcgatc cacgggtgcc
    10981 gggccacgag gccctcgagc gcgcggccgg gcaccgc
//
LOCUS       DATMCV010000644         3020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_767844, whole genome shotgun sequence.
ACCESSION   DATMCV010000644 DATMCV010000000
VERSION     DATMCV010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767844"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VNU03_14830"
     CDS             <1..1066
                     /locus_tag="VNU03_14830"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP75794.1"
                     /translation="LPYTSKHLDACLATVAEAIGWGDRDRKREAGRGPLRRGVGMAAY
                     CIERGGYAPFSAKADVVARPDGTVEVQAGVVEIGAGQITVLPMIAAEELGLPAERINI
                     HHGDTDGTHYAPSSHASRITSEVGPAVLQAASQVRQRLCELAGERLEVSPHDLVATGG
                     RIHVRGAAERGMTFEAACALMPGPEIRATGSRAPNPDDVIFRLFGAHGAEVDVDTETG
                     EVRVLRIVCSHDIGRPINPKLVESQQYGGAVMGLGYGLYEEAEMDGKSGVLLTPDVHQ
                     YRVPTSLETPLIEARNVEREDAFYPYSAKPVGEAPLVGVMPAVRNAIRHALGIGIDAL
                     PITPARILEALAAQRKAAEK"
     gene            1070..2116
                     /locus_tag="VNU03_14835"
     CDS             1070..2116
                     /locus_tag="VNU03_14835"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWP75795.1"
                     /translation="MLDELPPFDYLDAATLAEAVAALTEHGPRAQVLAGGTDLLGLMK
                     DRISGPGMPVPEVLVNVKRIPELHGIAEAADGGLRIGAAVTLADLERDARVMARFPAL
                     AQAAAAVATTQIRAMGTVGGNLCQRPWCWYFRHPQFPCLKRGGKQCFALPGSNRTYFG
                     VLGLGVCVMSHPSDLAPALIALDARVAIAGPAGSREVPIEQFFRGPRSRTETVLEPGE
                     ILVAVDVPAPAPGARSLFLKHRLRGTWDFALSEVAVSATPRSAAAGGSGAAGAGGWEN
                     VRVALGGVAPFPFRATAAEAVLAGHAPSESLLREAAAAAVARARPLTQNGYKIDLTRA
                     LVARALSALAADLG"
     gene            2121..2513
                     /locus_tag="VNU03_14840"
     CDS             2121..2513
                     /locus_tag="VNU03_14840"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75796.1"
                     /translation="MPRLILRRLLRAVAALLVVTAVVFTLLHLSGDPAYILLTPEATA
                     EDRAAFRAQYGLDRPLWVQYTRYLGRLAQGDFGQSLSFRIPAGSVALERLPRLWSSRW
                     PRWYWRWWSRCPRRCWPRCAGAPSSTAC"
     gene            2432..>3020
                     /locus_tag="VNU03_14845"
     CDS             2432..>3020
                     /locus_tag="VNU03_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75797.1"
                     /translation="MVLAVVVSLPAAVLAAVRRGTLFDRLLMGLTLIGQTVPTFWLGM
                     VMILVLAVRFHLFPASGRGGWLHLVMPATALALWLTALLARVTRSEMIEALEQDYVRT
                     ARAKGLAERGIAARHALKNALLPIITVMGLQFGGLLGGAVMTETVFAWPGVGTMILDA
                     IMKKDFPVVLAGVVIVAVGFIIVNLLLDLLYTMLDP"
BASE COUNT          365 a         1135 c         1093 g          427 t
ORIGIN      
        1 gctgccctat acctccaagc acctcgacgc gtgcctggcc acggtggcgg aggcgatcgg
       61 ctggggggac cgcgaccgca agcgcgaggc cgggcgcggc ccgctgcgtc gcggcgtcgg
      121 catggccgcc tactgcatcg agcgcggcgg ctacgcgccc ttctcggcca aggccgacgt
      181 ggtggcgcgc cccgacggca ccgtggaggt gcaagccggc gtggtggaga tcggcgccgg
      241 gcagatcacg gtgctaccga tgatcgcggc cgaggagctg gggctgcccg ccgagcgcat
      301 caatatccac cacggcgaca ccgacggcac gcactacgcg ccgtcgagcc acgcctcgcg
      361 catcaccagc gaggtgggcc ccgcggtgct gcaagccgcc tcccaggtgc gccagcggct
      421 ctgcgagctg gcgggggagc gcctggaggt ctcgccccac gacctggtgg ccacgggcgg
      481 gcgcatccac gtgcgcggcg cggccgagcg cggtatgacc ttcgaggccg cgtgcgcgct
      541 catgcccggg cccgagatcc gcgccaccgg cagtcgcgcg cccaacccgg acgacgtcat
      601 cttccgcctc ttcggcgccc acggcgccga ggtcgatgtc gacaccgaga ccggcgaagt
      661 gcgggtgctc cgcatcgtgt gctcgcacga catcggccgg cccatcaacc cgaagctggt
      721 cgagtcgcag cagtacgggg gcgcggtgat gggcctcggc tacggcctct atgaagaggc
      781 ggagatggac ggcaagagcg gcgtgctgct gacgccggat gtgcaccagt accgggtgcc
      841 gacgtcgctg gagacgccgc tgatcgaggc gcgcaacgtg gagcgggagg acgccttcta
      901 cccctactcg gccaagccgg tgggcgaggc gccgctggtc ggggtgatgc cggcggtgcg
      961 caatgcgatc cgccacgcgc tcggcatcgg gatcgacgcg ctgccgatca cgccggcgcg
     1021 catcctggag gccctggccg ctcagcgaaa ggctgcggag aagtagccga tgctggacga
     1081 gctgcccccg ttcgactacc tggacgccgc gaccctcgcg gaggcggtgg ccgcgctgac
     1141 cgagcacggc ccgcgcgccc aggtgctggc cggcggcacc gacctgctcg gcctcatgaa
     1201 ggaccgcatc agcggccccg gcatgccggt ccccgaggtg ctggtcaacg tcaagcgcat
     1261 cccggagctc cacggcatcg ccgaggccgc cgacggcggc ctgcgcatcg gcgccgcggt
     1321 cacgctcgcc gacctggagc gcgacgcgcg cgtcatggcg cgcttccccg ccctggccca
     1381 ggccgcggcc gcggtcgcca ccacccagat ccgcgcgatg ggcaccgtgg gcggcaacct
     1441 ctgccagcgg ccgtggtgct ggtacttccg ccacccgcag ttcccgtgtc tcaagcgggg
     1501 cggcaagcag tgcttcgcgc tgcccggcag caaccggacc tacttcggcg tgctgggcct
     1561 gggcgtgtgc gtgatgtcgc acccctccga cctggcgccg gcgctgatcg cgctggacgc
     1621 gcgggtggcg atcgccgggc cggccggctc gcgcgaggtg ccgatcgagc agttcttccg
     1681 cgggccccgc agccgcaccg agaccgtgct ggagccgggc gagatcctgg tcgcggtcga
     1741 cgtccccgcg ccggcgccgg gcgcgcggag cctcttcctc aagcaccgcc tgcgcggcac
     1801 ctgggacttc gccctctccg aggtggccgt ctcggccaca ccgcggtcgg ccgccgccgg
     1861 cgggtcgggc gcggccgggg cggggggctg ggagaacgtg cgcgtggctc tcggcggcgt
     1921 cgcgcccttc cccttccgcg ccaccgccgc cgaggcggtc ctggccggcc acgcgcccag
     1981 cgagtcgctc ctgcgcgagg ccgcggcggc cgcggtcgcc cgcgcccgcc cgctcacgca
     2041 gaacggctac aagatcgacc tgacccgcgc gctggtggcc cgcgcgctgt cggccctcgc
     2101 ggccgacctg ggctgacgcg atgccgcgcc tgatcctccg gcgcctgctg cgggcggtgg
     2161 cggcactcct cgtggtgacg gcggtggtct tcacgctgct ccacctctcg ggcgacccgg
     2221 cctatatctt gttaacgccc gaggccaccg ccgaggaccg cgccgccttc cgcgcccagt
     2281 acggcctgga ccgcccgctg tgggtccagt acacgcgcta cctcggccgc ctcgcgcagg
     2341 gcgacttcgg ccagtcgctc tcgttccgca tccccgccgg ctcggtggct ctcgagcgac
     2401 tgccgcgact ctggagctca cgctggccgc gatggtactg gcggtggtgg tctcgctgcc
     2461 cgcggcggtg ctggccgcgg tgcgccgggg caccctcttc gaccgcctgc tgatggggct
     2521 caccctgatc ggccagacgg tgcccacctt ctggctcggc atggtgatga tcctggtgct
     2581 cgcggtgcgc ttccacctct tccccgcctc cggccgcggc ggctggcttc acctggtgat
     2641 gccggccacc gcgctggccc tctggctcac cgcgctgctg gcccgcgtga cccgtagcga
     2701 gatgatcgag gcgctcgagc aggattacgt gcggaccgcg cgcgccaagg gcctggccga
     2761 gcgcggcatc gcggcccgcc acgcgctcaa gaacgcgctg ctgccgatca tcaccgtgat
     2821 ggggctacag ttcggtgggc tcctgggcgg cgcggtcatg accgagaccg tcttcgcgtg
     2881 gcccggcgtc ggcaccatga tcctcgacgc gatcatgaag aaggacttcc cggtggtgct
     2941 ggccggggtg gtcatcgtgg cggtgggctt catcatcgtc aacctgctgc tggacctgct
     3001 ctacaccatg ctcgacccgc
//
LOCUS       DATMCV010000645         2645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_697597, whole genome shotgun sequence.
ACCESSION   DATMCV010000645 DATMCV010000000
VERSION     DATMCV010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697597"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..430)
                     /locus_tag="VNU03_14850"
     tRNA            complement(355..430)
                     /locus_tag="VNU03_14850"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Ala,seq:cgc)
     gene            complement(495..1301)
                     /locus_tag="VNU03_14855"
     CDS             complement(495..1301)
                     /locus_tag="VNU03_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWP75798.1"
                     /translation="MEGVQLLFGIIPVVVILLYIFFSSFRILREYERAVIFRFGRRTN
                     AIFNPGGEGSGPGLVLLIPFIDKMVKVSLRTITMDVPPQDVITRDNVSVKVNAVIFFR
                     VLDAAKAVISVEDYLYATSQMAQTTLRSVLGQQEMDDLLASREKINEELTRIIDQHTS
                     PWGVKVAAVEVKNVDLPQDMQRAMSKQAEAERERRAKVINAEGEFQAAAKLGEAAEVL
                     MRFPIAVQLRYLQTMREVASERNTTTFFPLPLDLFTPLLRAFGDNPPPKS"
     gene            complement(1320..>2645)
                     /locus_tag="VNU03_14860"
     CDS             complement(1320..>2645)
                     /locus_tag="VNU03_14860"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HWP75799.1"
                     /translation="DAVRSANCRRLRGPLVVLLGLATLLSTAATVQAAAPVASIEMDS
                     AITPVTVRLLTTALDRAQSEGAQALIVQLNTPGGLERSMRSMVQTILGSPIPVIVYVS
                     PTGARAASAGVFITMAAHVAAMAPATNIGAAHPVAVGGSMDKEMSKKVENDAAAFART
                     IATERGRNAEWAEKAVRASVSATEREAVKLKVVDLVAESVPDLLAKIDGRTVKTSKGS
                     VTLATRDAPVKVIEVRFRDRFLALISDPNIAYLLMMAGMLGIFFELSNPGSILPGIIG
                     GICLILAFFAFQSLPVNWAGLLLLLFGVVLLIVEIKVVSHGVLTIGGVIAMLLGSIML
                     YDSPDVTGIRLSWFVIVPTVGTTAALVFFAVTMGIRALYRPSVTGSSAMVGRLGVTRT
                     ALQPDGQVLIDGELWRAVSPDPGVPAGEPVQVTAIDGLTLTVTRSPRRP"
BASE COUNT          439 a          874 c          934 g          398 t
ORIGIN      
        1 cgaacaccgt ggggcgcgtc acacgctatg gggcgttcct cgagctggtc tggcagccgc
       61 tgctgctcgc ggcgaaagtg ccgtaccgca agccacacgc catgcggcac tcctacgcga
      121 cgtggatgct cgaggccggc gccgacctcc ggtgggtcaa ggaccaactg gggcacgcct
      181 cgatcgagga gaccgagggg acctacggcc accttgagcg cgagcaccac gaaggccggg
      241 tgaacctcga cacggtactg agcggcgtcc actcacgtcc accggcgtcc acgctatccg
      301 acgccgatgg ggtgagcgaa ggtcaactac gcgattttcc gcgtgaggaa ttcttggtgg
      361 agggtaaggg attcgaaccc tcgacctcgg cgttgcgaac gccgcgctct cccaactgag
      421 ctaacccccc gttggatcgg cccattgtac tcggccgcgc tccggccgac aagccaaaga
      481 cgcgcccgga cggctcagct cttgggcggc ggattgtccc cgaacgcgcg caggagcggg
      541 gtgaagaggt cgagcggcag ggggaagaag gtcgtggtgt tccgctcgga ggcgacctcc
      601 cgcatcgtct gcagatagcg gagctgcacc gcgatcggga accgcatgag cacctcggcg
      661 gcctcgccca gcttggccgc ggcctggaac tcgccctccg cgttgatcac cttggcgcgc
      721 cgctcgcgct cggcctcggc ctgcttggac atggcgcgct gcatgtcctg gggcaggtcc
      781 acgttcttca cctcgaccgc ggccaccttc acgccccacg ggctcgtgtg ctggtcgatg
      841 atgcgggtca gctcctcgtt gatcttctcg cggctcgcca ggaggtcgtc catctcctgc
      901 tggccgagca cgctgcggag cgtggtctgg gccatctggg aggtcgcgta gaggtagtcc
      961 tccacgctga tcaccgcctt ggccgcgtcg agcacgcgga agaagatcac cgcgttgacc
     1021 ttcaccgaga cgttgtcgcg ggtgatcacg tcctggggcg gcacgtccat cgtgatcgtc
     1081 cgcaggctca ccttgaccat cttgtcgatg aacgggatca gcagcacgag ccccggtccc
     1141 gacccctctc cgcccggatt gaagatggcg ttggtccgcc ggccgaaccg gaagatgacc
     1201 gcgcgctcgt actcgcgcag gatccggaac gaggagaaga agatgtagag gaggatcacc
     1261 accaccggga tgatgccgaa gaggagctgg acgccttcca tgggcaatct cctgggatcc
     1321 tagggtcgtc gaggggagcg cgtgacggtg agggtgagcc cgtcgatggc ggtgacctgg
     1381 accggctcgc ccgcgggcac gccggggtca gggctgaccg cgcgccagag ctcgccgtcg
     1441 atgagcactt gcccgtccgg ctggagcgcg gtccgggtca cgccgaggcg gccgaccatc
     1501 gcggacgatc cggtcaccga gggccggtag agggcgcgga tgcccatggt caccgcgaag
     1561 aacacgaggg ccgcggtggt gcccacggtg gggacgatca cgaaccacga gagacggatg
     1621 ccggtgacgt ccggggagtc gtagagcatg atcgagccga gcagcatcgc gatcaccccg
     1681 ccgatggtga ggacgccgtg actcaccacc ttgatctcca cgatcagcag caccaccccg
     1741 aacaggagca ggagcaggcc cgcccagttg accggcaggc tctggaaggc gaagaaggcc
     1801 aggatcaggc agatcccgcc gatgatcccc ggcaggatgg agcccgggtt cgagagctcg
     1861 aagaagatgc cgagcatgcc cgccatcatg aggaggtagg cgatgttcgg gtccgagatg
     1921 agggccagga agcggtcgcg gaagcgcacc tcgatgacct tcaccggcgc gtcccgggtg
     1981 gcgagggtca ccgacccctt cgaggtcttt acggtccggc cgtcgatctt ggcgaggaga
     2041 tccggcacgc tctcggccac caggtccacc accttgagct tcaccgcctc ccgctcggtg
     2101 gccgagaccg aggcgcgcac cgccttctcg gcccactccg cgttgcgccc gcgctcggtc
     2161 gcgatggtgc gggcgaaggc ggccgcgtcg ttctccacct tcttgctcat ctccttgtcc
     2221 atgctcccgc ccaccgccac cggatgcgcg gcgccgatgt tggtggcggg cgccatcgcg
     2281 gccacgtggg ccgccatcgt gatgaagacg cccgcggagg cggcgcgggc gccggtgggc
     2341 gacacgtaga cgatgaccgg gatgggcgag cccaggatgg tctgcaccat cgagcgcatc
     2401 gagcgctcga gcccgcccgg ggtattgagc tgcacgatca gggcttgggc cccctcgctc
     2461 tgcgcgcgat cgagcgcggt ggtgagcagg cggaccgtca ccggagtgat ggccgagtcc
     2521 atctcgatgg aggcgaccgg cgctgcggcc tggacggtgg cggcggtgga aaggagcgtg
     2581 gcgaggccga gcaggacgac gagcgggccg cgaaggcgcc gacagttcgc cgatctcacg
     2641 gcgtc
//
LOCUS       DATMCV010000646         2865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_567429, whole genome shotgun sequence.
ACCESSION   DATMCV010000646 DATMCV010000000
VERSION     DATMCV010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567429"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VNU03_14865"
     CDS             complement(<1..991)
                     /locus_tag="VNU03_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWP75800.1"
                     /translation="MTPGAISPDQLTTLLQQRDAHALLDVRERAAFERGHIFRATPLP
                     RRLLETRLPMLVTAPGTLVVLYDDDGRIATLAAATLGEMGYTEVFVLTGGFDAWRAAG
                     RPVVQGLNVPSKVFGEHVLHDRKTPEVTPLELSARMAQGQDMVIVDTRTPEEYHRGCL
                     PGAWSMPGGELILRIGEVVERPDQTIVVHCGGRTRSYLGAESLRRMGLPNPIVAVKNG
                     TMGWQLDGLELERGASRWPPAPSAASRARAHDVATRLAAEDGTPLVSPDAVAERWSHR
                     DRENVSMLDVRTREEYEAGHVPGSIWAPGGQAVQATDEYVAVRAGWLVLICDGF"
     gene            complement(1025..1303)
                     /locus_tag="VNU03_14870"
     CDS             complement(1025..1303)
                     /locus_tag="VNU03_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75801.1"
                     /translation="MRLNRLNGSVQITRSSDSSYTLTYAPLGAGPGERPARRFAGARD
                     LERFLAGPLRVARREILAALVALDRNGSYLIAEIWLSPDELARAATLP"
     gene            1392..1589
                     /locus_tag="VNU03_14875"
     CDS             1392..1589
                     /locus_tag="VNU03_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75802.1"
                     /translation="MDRLPAFNALLLWGATFYLATHGVLPPASTEAEARELAVALREA
                     SVAWVGTRALAARDWASRLLG"
     gene            complement(1590..2624)
                     /locus_tag="VNU03_14880"
     CDS             complement(1590..2624)
                     /locus_tag="VNU03_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HWP75803.1"
                     /translation="MIDLFSDTLTKPTPEMRRFMCEAEVGDEQKGEDPTVTLLQEMVA
                     ELLGKEAAVYLPSGTMCNEIAVKVHTRPGDEVLADRTSHLIHFEGGGPGLLSGVLMTA
                     LEGDRGVFTGAQVRAGAHGRSRYAPPTRLVSVENTSNLGGGRVWPLAAVQDVAAAAKA
                     LGLATHMDGARLLNAVVASGTPARAYAAAVDSAWIDFSKGLGAPVGACLAGTRAFIEE
                     AWRYKQALGGAMRQAGIIAAGGVYALRHHVDRLAEDHEHARIFARGIAGLPGIALDPA
                     HVETNIVIFDVTAPGLTAQALADRLLVEAKVRLSVMGERRLRAVTHLDVSRKQIDAAV
                     EAFARALTGR"
BASE COUNT          358 a         1062 c         1052 g          393 t
ORIGIN      
        1 cgaagccgtc gcagatcagc acgagccatc ccgcgcgcac cgccacgtac tcgtcggtgg
       61 cctgcaccgc ctgcccgccc ggcgcccaga tcgagcccgg cacgtggccg gcctcgtatt
      121 cctcgcgggt gcgcacgtcg agcatcgaga cgttctctcg atcgcgatgg ctccaccgct
      181 cggcgaccgc gtcgggcgag accagcgggg tgccgtcctc ggcggcgagc cgcgtggcga
      241 cgtcgtgggc ccgcgcccgg ctcgccgccg acggggccgg cggccagcgg ctcgccccgc
      301 gctcgagctc gaggccgtcg agctgccagc ccatggtgcc gttcttcacc gccacgatcg
      361 ggttcggcag ccccatgcgg cgcagcgact ccgctccgag atacgaccgg gtgcggccgc
      421 cgcagtgcac gacgatcgtc tggtccggcc gctccaccac ctcgccgatg cgcaggatca
      481 gctcgccgcc gggcatgctc cacgcgccgg gcaggcagcc ccggtggtac tcctcggggg
      541 tgcgcgtgtc gacgatgacc atgtcctggc cctgggccat gcgcgcggac agctcgagcg
      601 gggtgacctc cggggtcttg cgatcgtgca gcacgtgctc gccgaagacc ttgctcggca
      661 cgttgaggcc ctgcaccacc gggcggcccg ccgcgcgcca ggcgtcgaag cccccggtga
      721 ggacgaacac ctccgtgtag cccatctcgc cgagggtggc cgcggccagc gtcgcgatcc
      781 gtccgtcgtc gtcatagagc acgacgagcg tgccgggcgc ggtcaccagc atcggcagcc
      841 gcgtctcgag caggcggcgg ggcaggggcg tcgcgcggaa gatgtggccg cgctcgaacg
      901 cggcgcgctc gcgcacgtcg aggagcgcgt gcgcgtctcg ctgctggagc agggtcgtga
      961 gctggtcggg ggagatcgcg ccgggggtca tgccccggac gctagccggg gacgagccgc
     1021 gctgtcaagg gagcgttgcc gcccgcgcca gctcgtcagg cgagagccag atctccgcga
     1081 tgaggtagct gccgttgcga tccagcgcga cgagcgcggc caggatctcg cgccgcgcca
     1141 cccgaagggg accggcgagg aaccgctcga ggtcccgcgc ccccgcgaag cgccgcgccg
     1201 gccgctcgcc cgggccggcc cccagcgggg cgtacgtcag ggtgtagctc gaatcggagc
     1261 tccgggtgat ctgcacggag ccgttcagtc tattcagtct catgcattgg cctcctcccg
     1321 ggtgcagacg ggaggggccg gggtgccgtt taccgtaaac gccggccctc ctgccggcgt
     1381 ctcaccggcg tatggaccgc ctgcccgcgt tcaacgccct gctgctctgg ggggccacgt
     1441 tctacctcgc cacccacggg gtactcccgc ccgcctccac cgaggccgaa gcccgcgagc
     1501 tggcggtggc gctgcgcgag gcgtccgtcg cgtgggtggg cacccgtgcg ctggccgcgc
     1561 gggactgggc ctcgcggctc ctcggctgac tagcgcccgg tgagcgcgcg cgcgaacgcc
     1621 tccaccgcgg cgtcgatctg cttgcgcgag acgtccaggt gggtcaccgc gcggagcctc
     1681 cgctcgccca tcacgctcag ccgcaccttc gcctccacga ggagccgatc ggcgagggcc
     1741 tgcgcggtga ggccgggggc ggtcacgtcg aagatcacga tgttggtctc gacgtgggcc
     1801 gggtcgagcg cgatgccggg cagccccgcg atgccccgcg cgaagatccg ggcgtgctcg
     1861 tggtcttccg cgagccggtc cacgtggtgg cggagggcgt acacgccgcc cgccgcgatg
     1921 atgccggcct ggcgcatggc cccgccgagc gcctgcttgt agcgccacgc ctcctcgatg
     1981 aacgcgcggg tgccggccag gcacgcgccc accggcgcgc ccaggccctt gctgaagtcg
     2041 atccacgccg agtcgaccgc ggccgcgtag gcccgcgcgg gggtgccgga ggcgaccacc
     2101 gcgttgagca gccgggcccc gtccatgtgg gtcgcgagcc cgagggcctt cgcggcggcc
     2161 gcgacgtcct gcaccgcggc gagcggccag acgcgcccgc cgcccaggtt gctcgtgttc
     2221 tccaccgaga ccaggcgcgt gggcggcgcg tagcgcgagc ggccgtgggc gcccgcgcgc
     2281 acctgggcgc cggtgaagac gccgcggtcg ccctcgagcg cggtcatcag caccccggag
     2341 agcagcccgg ggccgccgcc ctcgaagtgg atcaggtgcg aggtgcggtc ggccagcacc
     2401 tcgtcgcccg gccgcgtgtg caccttgacc gcgatctcgt tgcacatggt gccggacggc
     2461 aggtagaccg cggcctcctt gccgagcagc tcggccacca tctcctgcag cagggtcacg
     2521 gtggggtcct cgcccttctg ctcgtcgccg acctcggcct cgcacatgaa gcggcgcatc
     2581 tcgggggtgg gcttggtcag ggtgtcgctg aagagatcga tcacgatggg ctcctctagt
     2641 tggaaacgag ctcgggctga cccgagcatt cccaggccgc ggtcccccgt gccgcttgcg
     2701 gctcccggga cccggtgcga ttctacatcc gacatgcgga ttctcgtggt cgaagacgag
     2761 cgcaaggtcg ccgcgttcat ccgccaggga ctggtggagg aaggccacgc ggtcgcggtc
     2821 gcgtccgacg gggccgccgc gctcgacgcg atcgcggagg atccc
//
LOCUS       DATMCV010000647         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_169074, whole genome shotgun sequence.
ACCESSION   DATMCV010000647 DATMCV010000000
VERSION     DATMCV010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169074"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VNU03_14885"
     CDS             complement(<1..453)
                     /locus_tag="VNU03_14885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP75804.1"
                     /translation="MPLALGLFSMTTGPCSHPEGAARVAHAAEAAGFDSLWGGEHVVL
                     PDPQAPPSPLAPEDRITDPIVALAFLAAHTTRIRLGTGIIILPQRNPLVLAKQLATLD
                     VLSGGRLIFGVGVGYLEPEFRALGIPFETRGPMTDEYLAAMRTIWSQER"
     gene            complement(453..1295)
                     /locus_tag="VNU03_14890"
     CDS             complement(453..1295)
                     /locus_tag="VNU03_14890"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWP75805.1"
                     /translation="MGDWLETYRGTVFRWEVDHNDHFTVAYYFARIADAGLGMLADLG
                     LSPLDAPTTDCFVRYQRELRVGDVMHVESGVVHAEPGAIVLGHKLFDTGAATLCTTVE
                     QRVRVPATLPPDAARRVEARRVAWDGPARERRPRPIGLAGFRDSARDTVKPAELDVSG
                     GAALSHYIHRFSAANGHAIASFGMTPAYMRGEHRGFSTFEFQLELGEPLRAGDLVRVR
                     SGVLHVGSSSLRLLHVMTREPGGEVVASLEQSGVHFDQDARRPAPLPPPLRERALGLL
                     LPAP"
     gene            complement(1308..2291)
                     /locus_tag="VNU03_14895"
     CDS             complement(1308..2291)
                     /locus_tag="VNU03_14895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP75806.1"
                     /translation="MEQRTLGKSTLAVSAIGLGCMSMSNVYGKCDDAESIAVVHRALD
                     LGVNFLDSSDMYGWGQNEELLGRALRGKRDGVVLATKFGNLRKPDGTAGVNGRPEYVP
                     QACEASLKRLGVDSIDLYYQHRVDPGVPIEDTVGAMSRLVEQGKVRYLGLSEASPATV
                     RRAHAVHPITALQSEFSMLYRVEAEETLPVLRELGISFVAYSPLGRSLLTGSTRTAAE
                     IPTDDRRRDHPRFQDENLQKNLDLLKPLLDMAKQKGCTPGQLALAWLLARGRDIVPIP
                     GTKRKARLDENAAAAAVSITPAEVAALQDAVPTGAAAGTRYPGPAMKSVYI"
     gene            2370..>3229
                     /locus_tag="VNU03_14900"
     CDS             2370..>3229
                     /locus_tag="VNU03_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWP75807.1"
                     /translation="MVHDVTLLRDIALGIIFAAVLGHAARLLRQPLLLGYIAGGILLS
                     SQMGFGLVSDPESIELISEIGLILLLFIIGLEIDLRELKRLGRSMLTLGVGQFVINAL
                     LGAAFFSWLGYRVGDGRFDLVYLAIVISLSSTLIVVKLLREKFELKILSGRLTLGVLV
                     VQDIWAILFMAVQPSLAEPGVLRIAQSIAGGAVLVGVAFLASRFILAPLLEVSASRPE
                     LVLISSVAWCFGIAGFADQLGLSREMGALIAGVSISAFPYGSDVISKVTGVRDFFVTL
                     FFVALGMKVP"
BASE COUNT          426 a         1112 c         1196 g          495 t
ORIGIN      
        1 ccgctcctga ctccagatcg tgcgcatcgc ggccaggtac tcgtcggtca tcgggccccg
       61 cgtctcgaac ggaatcccga gcgcccggaa ctcgggctcg aggtatccga cgcccacgcc
      121 gaagatcagg cggccgccgg agaggacgtc gagcgtcgcg agctgcttgg ccaggaccag
      181 cgggttgcgc tggggcagga tgatgatgcc ggtgccgagc cggatccggg tggtgtgcgc
      241 ggcgaggaag gcgagcgcca cgatggggtc ggtgatgcgg tcctccggcg ccaggggcga
      301 cggcggagcc tgcgggtccg ggagcaccac gtgctcgccg ccccagagcg aatcgaagcc
      361 ggccgcttcc gcggcgtggg ccacccgggc cgccccctcc gggtgcgagc acggcccggt
      421 ggtcatcgag aagaggccga gggcgagcgg catcagggcg cgggcagcag caggccgagc
      481 gcgcgctcgc gcaggggcgg gggcagcggg gccgggcgcc gcgcgtcctg atcgaagtgc
      541 acgccggact gctcgagcga ggccaccacc tcgccgcccg gctcgcgggt catcacgtgc
      601 agcagccgga gcgaggagct gcccacgtgc aggacgccgg atcgcacccg cacgaggtcg
      661 cccgcgcgca gcggctcacc cagctcgagc tggaactcga aggtcgagaa gccgcggtgc
      721 tcgccgcgca tgtaggcggg ggtcatgccg aacgaggcga tggcgtggcc gttggcggcc
      781 gagaagcggt ggatgtagtg cgagagcgcg gcgccgcccg acacgtccag ctcggccggc
      841 ttcacggtgt cgcgcgcgct gtcgcgaaag cccgcgagcc cgatcgggcg gggacgccgc
      901 tcacgggccg ggccgtccca ggccacgcgc cgcgcctcga cccgccgcgc ggcgtcgggc
      961 ggcagcgtgg ccggcacgcg cacccgctgc tccacggtgg tgcagagcgt cgccgcgccg
     1021 gtgtcgaaga gcttgtggcc gagcacgatc gcgccgggct cggcgtggac caccccgctc
     1081 tccacgtgca tgacgtcgcc cacccgcagc tcgcgctggt agcgcacgaa gcagtcggtg
     1141 gtgggggcgt cgagcgggct caggccgagg tcggcgagca tcccgaggcc cgcgtcggcg
     1201 atgcgggcga agtagtacgc caccgtgaag tggtcgttgt gatccacttc ccagcggaag
     1261 acggttccgc ggtacgtctc gagccagtcg cccacgccgg cgccggccta gatgtagacc
     1321 gacttcatgg cggggcccgg gtagcgggtg ccggcggcgg cgccggtggg caccgcgtcc
     1381 tggagcgccg cgacctcggc cggggtgatc gacaccgcgg cggcggccgc gttctcgtcg
     1441 aggcgagcct tccgcttggt ccccgggatc ggcacgatgt cgcgcccgcg cgcgagcagc
     1501 caggccagcg cgagctggcc cggagtgcag cccttctgct tcgccatgtc gagcagcggc
     1561 ttcagcagat ccaggttctt ctggaggttc tcgtcctgga agcgggggtg atcccgccgg
     1621 cggtcgtcgg tcgggatctc ggccgcggtg cgggtgctcc cggtcagcag gctgcggccg
     1681 agcggggagt aggcaacgaa cgagatgccg agctcgcgga ggacgggcag ggtctcctcc
     1741 gcctccaccc ggtagagcat ggagaactcg gactggagcg cggtgatcgg atgcaccgcg
     1801 tgcgcgcggc gcacggtggc cggggaagcc tcggagagac cgaggtaccg caccttgccc
     1861 tgctcgacca ggcgggacat ggcgccgacc gtgtcctcga tgggcacgcc ggggtcgacc
     1921 cggtgctggt agtagagatc gatgctgtcg acccccagcc gcttgaggct cgcctcgcag
     1981 gcctgcggca cgtactcggg ccgcccgttg accccggcgg tgccgtccgg cttccgcagg
     2041 ttgccgaact tggtggccag caccaccccg tcgcgcttgc cccggagggc ccggcccagc
     2101 agctcctcgt tctggcccca cccgtacatg tcggaggagt ccaggaagtt gacgcccagg
     2161 tcgagggcgc ggtggacgac cgcgatggac tccgcgtcgt cgcacttgcc gtagacgttg
     2221 gacatggaca tgcagccgag tccgatggcc gagacggcca gtgtgctctt gccgagggta
     2281 cgctgctcca cgagatcctc ctccgggtcg ggaaagttgg ttaggatacg gcgaccgatt
     2341 ctatcccaag acggaggggc gggcgaccgg tggttcacga cgtgacgctg ctgcgcgaca
     2401 tcgccctcgg catcatcttc gccgcggtgc tcggtcacgc cgcccgcctg ctccggcagc
     2461 cgctcctcct gggctacatc gcgggcggca tcctgctcag ctcccagatg ggcttcggcc
     2521 tcgtctccga cccggagagc atcgagctga tctccgagat cggcctcatc ctgctcctgt
     2581 tcatcatcgg gctcgagatc gacctgcgtg agctcaagcg cctcggccgc tcgatgctga
     2641 ccctcggggt cggccagttc gtcatcaacg cgctgctcgg ggccgccttc ttctcctggc
     2701 tcggctaccg ggtgggggac ggtcgcttcg acctcgtcta tctggccatc gtgatctcgc
     2761 tcagctcgac cctgatcgtg gtcaagctgc tgcgcgagaa gttcgagctc aagatcctct
     2821 ccggccgcct caccctgggg gtgctggtgg tgcaggacat ctgggccatc ctcttcatgg
     2881 cggtgcagcc gagcctggcc gagccgggcg tgctgcgcat cgcccagtcc atcgcggggg
     2941 gcgcggtgct ggtgggcgtc gcgttcctgg ccagccggtt catcctcgcc ccgctgctgg
     3001 aggtctcggc gagccggccg gagctggtcc tgatctcctc ggtggcgtgg tgcttcggca
     3061 tcgcgggctt cgcggaccag ctcgggctgt cgcgcgagat gggcgcgctg atcgcggggg
     3121 tgagcatctc cgccttcccc tacggctccg acgtgatctc caaggtcacc ggggtgcgcg
     3181 acttcttcgt caccctgttc ttcgtggcgc tcggcatgaa ggtgccgat
//
LOCUS       DATMCV010000648         2090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_902451, whole genome shotgun sequence.
ACCESSION   DATMCV010000648 DATMCV010000000
VERSION     DATMCV010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902451"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VNU03_14905"
     CDS             complement(<1..217)
                     /locus_tag="VNU03_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75808.1"
                     /translation="MTHQPAVTPAPVASAPQAPFLGLSTLWVQITGIWCNLECRHCIN
                     ASGPRAPWLKPLDGATIRRAIGEAEALG"
     gene            complement(268..1197)
                     /locus_tag="VNU03_14910"
     CDS             complement(268..1197)
                     /locus_tag="VNU03_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HWP75809.1"
                     /translation="MIHQLDAGQVQYEWNNAIAPRIEIEPGDTVVFETRDAADRFYSR
                     TSTHADVLARGPFRGHPLTGPVRVRGARPGDALVVEILEVKPADFGWTAIRPGRGLLP
                     EADFAKPFLQIWDLANGTHARAGKRLAVPLDPFAGVMGTALDLPGGHSTMPPRKNGGN
                     MDIKQLTAGSTLYLPVWVDGALFCVGDTHGIQGDGEVCVTAVEMGGRVTARLGLERDR
                     GLAEPQLRTTRPPSSTAPGPWFATTAHGPDLFAASQQAVRYMIDHLVRERGLSREEAY
                     ILCSVTVDLKISEIVDAPNWIVSAFLPEGVFVV"
     gene            1254..>2090
                     /locus_tag="VNU03_14915"
     CDS             1254..>2090
                     /locus_tag="VNU03_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75810.1"
                     /translation="MLRATMSTSLEAPTPGPRVFREREPGQPDNGRDPHPAVRDGSRS
                     EPPTRGVFAAIFLISAATLLLELTLTRIFDVVLWTNLANFIVSSAIFGLGLGGIAVML
                     RPSLATGHRPAGWAALAFAVAVLLLLPAVTLLPFDFGAILTQPVRQLVSFAALYAALL
                     APFFAAGVVIASILTRHARRVDHLYFWDLLGAGLGALGIIWLPTLIGPAAMLVLIAAA
                     GVVVAGLLAPSRSRTRVGVLCLTLGLLGSALFASDRIDFQTHVAKGTLQSGDALRQAE
                     YSR"
BASE COUNT          265 a          762 c          739 g          324 t
ORIGIN      
        1 cgccgagcgc ctccgcctcg ccgatggccc gccggatcgt cgcgccgtcg aggggcttga
       61 gccacggcgc gcgcggcccg ctcgcgttga tgcagtggcg gcactcgaga ttgcaccaga
      121 tgccggtgat ctgcacccag agggtggaga gcccgaggaa cggcgcctgc ggggccgagg
      181 ccaccggggc cggcgtgacg gcgggctggt gcgtcatccg gcccagcctg cgcgtctcac
      241 ggcggaggat gtcacagcgg cgtcaaatca gacgacgaac acaccctcgg gcaggaaggc
      301 ggagacgatc cagttgggcg cgtccacgat ctcgctgatc ttgagatcga ccgtcacgct
      361 gcacaggatg taggcctcct cccgggagag gccgcgctcg cgcaccaggt ggtcgatcat
      421 gtagcgcacc gcctgctggg aggccgcgaa gagatcgggg ccgtgcgcgg tggtggcgaa
      481 ccacggcccc ggcgcggtcg acgagggcgg ccgcgtcgtg cgcagctgcg gctcggcgag
      541 gccccggtcg cgctcgagcc cgaggcgcgc ggtcactcgg ccgcccatct ccaccgcggt
      601 gacgcacacc tcgccgtcgc cctggatccc gtgggtgtcg cccacgcaga acagcgcccc
      661 gtccacccat accggcaggt agagcgtcga tccggcggtc agctgcttga tgtccatgtt
      721 gccgccattc ttccgtggcg gcatcgtgct gtgcccgccc ggcaggtcga gcgcggtccc
      781 catcacgccc gcgaagggat cgagcggcac cgcgaggcgc ttgccggccc gcgcgtgggt
      841 gccgttggcc agatcccaga tctgcaggaa tggcttcgcg aaatccgcct cgggcagcag
      901 gccgcggccg ggccggatcg cggtccagcc gaagtcggcc ggcttcacct ccaggatctc
      961 caccaccagc gcatcgcccg ggcgcgcgcc gcgcacccgc accggcccgg tcagggggtg
     1021 cccccggaag ggaccgcgcg cgagcacgtc ggcgtgggtg gaggtgcgtg agtagaaccg
     1081 gtcggccgcg tcgcgggtct cgaagaccac cgtgtcgccc ggctcgatct cgatccgcgg
     1141 ggcgatcgcg ttgttccact cgtactggac ctgcccggcg tcgagctggt ggatcatggc
     1201 gctcgaagta tacacgcggc ggcggggcct tgttgaggcg gagccggcgg ggtatgctcc
     1261 gcgccaccat gtcgaccagc ctggaggcgc cgaccccggg gccgcgggtc ttccgggagc
     1321 gcgagccagg gcagccagac aatggccggg acccgcatcc ggcggtccgt gacggctccc
     1381 gatccgagcc gcccacccgc ggggtcttcg cggcgatctt cctcatctcg gccgcaacac
     1441 tgctcctcga gctgacactc acccgcatct tcgacgtggt gctgtggacc aacctggcca
     1501 acttcatcgt gagcagcgcg atcttcggcc ttgggctggg cggtatcgcg gtcatgctgc
     1561 ggccgtccct cgcgacggga caccggcccg cggggtgggc cgcgctcgcg ttcgcggtcg
     1621 cggtgctgct gctcctgccc gcggtcaccc tgctgccgtt cgacttcggc gcgatcctga
     1681 cccagccggt tcgacagctc gtgagcttcg ccgcgctcta cgccgccctg ctcgcgccgt
     1741 tcttcgcggc cggggtggtc atcgcctcga tcttgacccg ccacgcgcgc cgggtcgacc
     1801 acctctactt ctgggatctc ctcggtgcgg gcctcggcgc gctcggcatc atctggctcc
     1861 ccaccctgat cgggccggcc gcgatgctcg tcctgatcgc ggcggccggg gtggtggtgg
     1921 ccgggctcct cgcgccctcc cgcagccgga cccgcgtcgg cgtcctgtgc ttgacattgg
     1981 gcctgcttgg atcagcgcta ttcgcctccg accgtatcga cttccagaca cacgtagcga
     2041 agggcaccct gcagtccggg gatgccttgc ggcaggccga gtactctcgg
//
LOCUS       DATMCV010000649         4171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_169103, whole genome shotgun sequence.
ACCESSION   DATMCV010000649 DATMCV010000000
VERSION     DATMCV010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169103"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VNU03_14920"
     CDS             complement(<1..622)
                     /locus_tag="VNU03_14920"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWP75811.1"
                     /translation="MSELPRPVADLCAALGGQPGVLAVALGGSRAAGTADAASDWDLG
                     LYYRGEIDLTVLARHGEVHAPGSWGRLMNGGTWLTVDGLKVDVLLRDLDVVRHWSAEA
                     EQGRYEVDALLGYVAGAPTYGLMAELAVSRALVGALPEVSAYPAALAEAGARRWRVHA
                     EFSLEHARMRAGRGDVTGAAGQAAKAVLEAAHARACRERLWVLNEKT"
     gene            complement(619..1635)
                     /locus_tag="VNU03_14925"
     CDS             complement(619..1635)
                     /locus_tag="VNU03_14925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75812.1"
                     /translation="MGLRLPALNDPLIECHAHVLPDAALARFPDGGDPRYVTAERRVL
                     GRMLEAQEAAGVTHALVSDSFFMESAAVALPAWSPSDRARLYNDTLAALIARYPGRLL
                     GLGCVDPFAGDAAARELERMVDELGFLGALVNPTDAPTGGRRYLDDEACGPLLAAAAA
                     RGRPLFVHPSRELPAGEHFDDFVLSLIVGRPTQTAVCAARLIFTGTLDRHPRLPLLLA
                     HGGGVLPAMAGRLDATWRAYRPDRWQGPDVLSSPPSTYLRRFHVDTNVWSVPALRLLL
                     DVLGPDRLLVGNDQPPVWFPLGESLALLDQLALPAAEREAIRWGNAARLFGIAAPRAA
                     AARA"
     gene            complement(1611..3530)
                     /locus_tag="VNU03_14930"
     CDS             complement(1611..3530)
                     /locus_tag="VNU03_14930"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HWP75813.1"
                     /translation="MAVVRLEVTRREPVLGGAPFGAGGAYEKVEGVLHFAVDPSARVH
                     EPIADLGQAPRNARGLVESSADFSLLRPTGGGSCRLLLDVPNRGRKIALGMFNSAPRS
                     NDPATGADFGNGFLMRHGYAVAWIGWQPDVPRRDGMMALTVPRVPGVSERVRCEFRPN
                     ARVGVLPLADRYHIPHPVARLDDPEAELHVREHAEAAAVPVPRGAWRFPDAGSVALDG
                     GFEPGRIYECYYRAENPPLVGLGFTAVRDTAAFLRWASAGEGNPCAGQIDRSYVFGVS
                     QSGRFLRHLLYLGLDEDEAGRPAWDAVVPHVAGGRRGEFNCRFGQPSLNAVHAVGSLF
                     PFTDGEQDDPVTGQRGALLHRLAARSRPPRIFTINTAAEYWRGDGSLVHTDVAATRDV
                     SPPAWVRQYFFAGTQHTPGAIPPPPADPNTGGRGRHTFSVVDYSPLLRAALVNLDRWV
                     SEGVEPPPSAVPRLADGSAVAAETTRPVYAAIPGMRFPDRVQRPVRLDFGPRVEQGIV
                     DLPPKIGAPYVTVVSAVDADGNDRAGIRPPELRVPVATFTGWNPRHPDQGAPGDLMSM
                     MGSTLPFARTAAERATSGDPRPSLEERYGTRDRYLARVRSDAQAMVADRHLLAEDVEA
                     VVERAGALWDFVCPR"
     gene            complement(3544..>4171)
                     /locus_tag="VNU03_14935"
     CDS             complement(3544..>4171)
                     /locus_tag="VNU03_14935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP75814.1"
                     /translation="GHGDSDPATVYGPIAAFDDLNGVVDQLGLAPAVMVGLSMGGRNA
                     IYFTAKRPDAVQKLVILDIGPEISKRASQATPGPPEPEFWDSIEQAAQHLLRGNAYPG
                     IHYYRWVAASSLRQRPDGRLVWAWHPSVKALRSTVEVDWWGLLRSIPVPTLVLRGEES
                     PILDRDVAEKMARELPKGAFVEIPRAVHTLHEDNPDAVLAALRSFLGF"
BASE COUNT          570 a         1550 c         1565 g          486 t
ORIGIN      
        1 gcgtcttctc gttgagcacc cagaggcgct cgcgacaggc gcgggcgtgc gcggcctcga
       61 gcaccgcctt ggcggcctgg cccgccgcgc cggtcacgtc cccgcgcccg gcccgcatgc
      121 gcgcgtgctc caggctgaac tcggcgtgga cgcgccagcg gcgcgcaccg gcctcggcga
      181 gcgcggcggg gtaggcggag acctcgggca gcgcccccac gagcgcgcgg ctcaccgcca
      241 gctcggccat caagccgtac gtcggcgcgc ccgccacgta gcctagcagc gcgtcgacct
      301 cgtagcgtcc ttgctccgcc tctgcgctcc agtgccggac cacgtcgaga tcgcggagca
      361 gcacgtcgac cttcagcccg tcgacggtga gccacgtccc gccgttcatc agacgtcccc
      421 acgagcccgg cgcatgcacc tcgccgtggc gcgccagcac cgtcagatcg atctctcccc
      481 ggtagtagag gcccaggtcc cagtcgctcg ccgcgtccgc ggtccccgcg gcgcgggagc
      541 cgccgagcgc caccgccagg acgccgggct gaccgccgag cgccgcgcag agatcggcca
      601 ccggtcgcgg cagctcgctc atgcccgggc ggcggcggcc cgtggcgcgg cgatcccgaa
      661 gagccgcgcc gcgttgcccc agcggatcgc ctcgcgctcg gcggcgggca gcgcgagctg
      721 atcgagcagc gcgagcgatt cgccgagcgg gaaccacacc ggcggctggt cgttgccgac
      781 cagcaggcgg tcggggccga gcacgtccag caggaggcgc agcgcgggga ccgaccacac
      841 gttggtgtcg acgtggaagc ggcgcagata cgtggacggg ggtgacgaga gcacgtccgg
      901 gccctgccat cggtccggcc ggtaggcgcg ccaggtcgcg tcgagccggc cggccatggc
      961 ggggagcacg ccgcccccgt gcgcgagcag gagcggcagg cgcggatgcc gatcgagcgt
     1021 gccggtgaag atcaggcgcg ccgcgcacac cgcggtctgc gtcggacgcc cgacgatcag
     1081 cgagagcacg aagtcgtcga agtgctcccc ggcgggcagc tcacgcgacg gatggacgaa
     1141 gagcggccgg ccccgcgccg ccgcggcggc gaggagcggg ccgcacgctt catcgtcgag
     1201 atagcggcgg ccgccggtcg gcgcgtcggt cgggttcacg agggcgccca ggaaaccgag
     1261 ctcgtccacc atccgctcca gctcgcgggc agccgcgtcg cccgcaaagg gatccacgca
     1321 gcccagcccg aggagacggc ccgggtagcg cgcgatcagc gccgcgagcg tgtcgttgta
     1381 gaggcgcgcc cggtcggacg ggctccaggc gggcagcgcg accgcggccg actccatgaa
     1441 gaaggagtcg gagaccaggg cgtgggtgac tccggcggcc tcctgcgcct ccagcatccg
     1501 cccgagcacc cgccgctccg cggtcacgta gcgcgggtcg ccgccatcgg gaaaccgggc
     1561 cagcgccgcg tcgggcagga cgtgcgcgtg gcactcgatc agcgggtcgt tcagcgcggg
     1621 cagacgaagt cccagagcgc tccggcgcgc tcgaccaccg cctccacgtc ctcggccagc
     1681 aggtgccggt ccgcgaccat ggcctgcgcg tcgctccgga cccgggccag gtagcggtcg
     1741 cgggtgccgt accgctcctc gagagagggc cgcgggtctc cgctcgtcgc ccgctccgcc
     1801 gcggtgcgcg cgaacggcag ggtggagccc atcatcgaca tgagatcgcc gggcgcgccc
     1861 tgatccgggt gccgcgggtt ccagccggtg aacgtggcga ccggcacccg gagctccggc
     1921 ggccggatgc cggcccggtc gttgccgtcg gcgtccaccg cggacaccac cgtgacgtag
     1981 ggcgcgccga tcttgggcgg caggtcgacg atgccctgct cgacgcgcgg gccgaagtcg
     2041 agccgcaccg gccgctggac gcggtcggga aagcgcatcc cggggatggc agcgtacacc
     2101 ggacgcgtcg tctcggcggc caccgcggag ccgtcggcca ggcgcgggac ggcgctcggc
     2161 ggcggctcga cgccctcgct cacccagcgg tcgaggttga cgagcgcggc gcgcaggagc
     2221 ggagaatagt cgacgacgct gaaggtgtgg cgcccgcgcc cgcccgtgtt cgggtcggcg
     2281 ggcggcggcg ggatcgcgcc gggggtgtgc tgggtgcccg cgaagaagta ctggcggacc
     2341 cacgcgggcg ggctcacgtc gcgcgtggcc gccacgtcgg tgtgcacgag cgagccgtcg
     2401 ccgcgccagt actcggcggc ggtgttgatg gtgaagatgc gcggcgggcg ggagcgggcc
     2461 gcgagccggt ggagcagcgc cccgcgctgg ccggtgaccg gatcgtcctg ctctccgtcg
     2521 gtgaacggga acaggctgcc gaccgcgtgc accgcgttca gcgagggctg gccgaagcgg
     2581 cagttgaact ccccgcggcg cccgcccgcc acgtgcggga ccaccgcgtc ccacgcgggt
     2641 cggcccgcct cgtcctcgtc gagccccagg tagagcaggt gccgcaggaa gcgcccgctc
     2701 tgcgagacgc cgaacacgta ggaccggtcg atctgtcccg cgcacgggtt cccctccccc
     2761 gcgctcgccc atcggagaaa agccgcggtg tcgcgcacgg cggtgaagcc cagaccgacc
     2821 agcggcgggt tctcggcccg gtagtagcac tcgtagatcc gcccgggctc gaagccgccg
     2881 tcgagcgcga ccgagcccgc gtcggggaat cgccacgcgc cccgcggcac cgggaccgcg
     2941 gcggcctcgg cgtgctcgcg aacgtgcagc tccgcctccg ggtcgtccag gcgcgccacc
     3001 gggtgcggga tgtggtagcg atcggcgagc ggcagcacgc cgacgcgggc gttcgggcga
     3061 aactcgcagc gcacccgctc ggacacgccg ggcacccgcg gcaccgtgag cgccatcatg
     3121 ccgtcgcgcc gcggcacgtc cggctgccag ccgatccagg ccaccgcgta gccgtggcgc
     3181 atcaggaagc cgttgccgaa gtccgcgccg gtggcggggt cgttcgagcg cggcgcgctg
     3241 ttgaacatgc cgagcgcgat cttgcgcccg cgattgggca cgtccagcag caggcggcaa
     3301 ctcccgccgc ccgtcgggcg cagcagggag aaatcggccg aggactcgac cagcccgcgc
     3361 gcgttgcggg gagcctgccc gagatcggcg atcggctcgt gcacccgcgc cgacgggtcc
     3421 accgcgaagt ggagcacgcc ctcgaccttc tcgtaggcgc cccccgcgcc gaacggcgcc
     3481 ccgcccagga ccggctcgcg ccgcgtcacc tcgagtctca cgaccgccat ggggattctc
     3541 cgatcagaag ccgaggaacg agcggagcgc ggcgagcacc gcgtccggat tgtcctcgtg
     3601 cagggtatgg accgcgcggg gaatctcgac gaaggcgccc ttcggcagct cgcgcgccat
     3661 cttctcggcc acgtcgcggt cgaggatcgg gctctcctcg ccgcgcagca ccagcgtggg
     3721 caccgggatc gaccgcagga ggccccacca gtccacctcg acggtggagc gcagcgcctt
     3781 cacgctcgga tgccacgccc acacgaggcg gccgtccggt cgctggcgca ggctcgaggc
     3841 ggccacccac cggtagtagt gaatgccggg gtacgcgttg ccgcggagca ggtgctgggc
     3901 cgcctgctcg atgctgtccc agaactccgg ctccggcggc cccggcgtgg cctgggaggc
     3961 acgcttgctg atctcggggc cgatgtcgag gatgacgagc ttctgcaccg cgtccggccg
     4021 cttggccgtg aagtagatcg cgttgcgacc acccatcgag aggccgacca tcaccgcggg
     4081 cgcgagcccc agctggtcga ccacgccgtt gagatcgtcg aacgcggcga tcggcccgta
     4141 gacggtcgcg gggtcgctgt cgccgtgccc g
//
LOCUS       DATMCV010000650         5376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_169135, whole genome shotgun sequence.
ACCESSION   DATMCV010000650 DATMCV010000000
VERSION     DATMCV010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169135"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VNU03_14940"
     CDS             <1..357
                     /locus_tag="VNU03_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP75815.1"
                     /translation="KTRRQFGVPIGSFQALQHRMVDMVISYEQARSMACLACVKVDSA
                     PPDERRRAVSAAKIKVADACRHVSQEAVQLHGGMGMTEELKVSHTFRRLTMIGQTFGD
                     AEHHLERYAVADGAGA"
     gene            541..1524
                     /locus_tag="VNU03_14945"
     CDS             541..1524
                     /locus_tag="VNU03_14945"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HWP75816.1"
                     /translation="MSPPSGPGPEPPAVSTVRRPTFHGNGGTLFGIHVVNVLLTIVTL
                     GIYYFWAKTRIRSYLSGQTEIEGDRFAYHGTARELLLGTIKAVLVFGLPILFLNMIRD
                     VLDVPPVAKVIAGVVSGSLFFVFFPIAMVGARRYRLSRTSWRGIRFSFRGHVWALIKI
                     FILGSFLTGLTLGLYYPFFLVSRQAFMVAHSYFGSERFDFNGRGRELFGPFLVAILLT
                     LPTLGLCWVWYVAFKRRYFWDHTTFGTARFSCSVTGGALLGLWVVNLILLVGTLGIAW
                     PWVRVRNIHFAFRNLALVGALDLARIQQQAQQVGSIGEGLSGFFDSGFDLA"
     gene            1540..2634
                     /locus_tag="VNU03_14950"
     CDS             1540..2634
                     /locus_tag="VNU03_14950"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWP75817.1"
                     /translation="MRTDWQGIYLDGQTPARHAATVRLMREALEISPAGGAPRLWPYR
                     ELRQTQGFYAGEEVRIERGGDLPETLLIPDSAFLESLHEVAPGLRLRFHDPRRRRARV
                     RWTIAAAAAVLAVTAAIYVWGIPALAALVAPRVPVAWEESVGRSAIAYMAPPDRRCGD
                     PRLGAAMDEIVRRLTASAPPSPYTLRVYVVNRAVVNAHALPGGNVVIFKGLLERTRTP
                     EELAGVVAHELQHVLRRHTTRAVIQDASTGLLLMALTGDVTGPLAYGLQTARTLGELR
                     YSRRAEDEADTEGMKMLLAARIDPAGMIDFFDTIGQEEGRQAKALTYFSTHPLAADRI
                     ARLKAMAAAWKGTPEPLLPGEDWAALAKRC"
     gene            complement(2646..4262)
                     /locus_tag="VNU03_14955"
     CDS             complement(2646..4262)
                     /locus_tag="VNU03_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWP75818.1"
                     /translation="MMDYPLTLTQFFERTRRLFHRKRMATRVPGMGLQWYSYADYADR
                     VCRLAGALRGLGLQKGTRVGTFAWNSHRHMEVYFAAPLMGMVLHTVNIRLSAQDITYI
                     VNHAGDEVLIVDASLWSILEPIRNDLTTVKHIIIMKDTPTAHVPPGALDYEQLVAGTD
                     PVTEWPRLEETDAAGICYTSGTTGHPKGVVYTHRAIYLHCFASSTVDVLGICERDVIL
                     HIVPMFHANAWCVPFAGVMNGATQIFGGPNPQPRDIIEIVHHEKVTVVGAVPTVWIAI
                     QGILEKEPQWDISSIRCIPIGGSAAPRTLIELFDKKYGAYMLHAWGMTEMTPLGSVCR
                     PRSYMDALPEDERYAVRAKQGAPVAGVDMRIVDDAGNILPWDGHSAGEIQVRGPWVTS
                     GYYNNPEGAAQFTGDGWFRTGDVAALDPDGYIQITDRTKDLIKSGGEWISSVDMETTI
                     MGHPKVLEAAVIAVPHERWVERPLACIVPKPEHKGSLTADEIYEYLKPRVAKWWLPDE
                     VVFIEAVPKTSVGKFDKKVLRDRFKAWKPK"
     gene            complement(4279..5172)
                     /locus_tag="VNU03_14960"
     CDS             complement(4279..5172)
                     /locus_tag="VNU03_14960"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWP75819.1"
                     /translation="MAVKIAGRYRGRVTAWTRRGLTLGAALALALIGPLASGRGPGSG
                     EARADGIMLIEAGAFWMGSDSTVPDEGPMHRVYVRDFWLDRNKVTNREVARFLEARGL
                     RSPEGMPYFDQDDPDARIHRREGRFIADPGFEEHPAVEVSWFGARDYCLWLGRRLPTE
                     AEWEKAARGLDRHRYPWGDQAPDAERAIVGRPYNATERGDLRPAGAGPTGVQDLLGNL
                     REWTGSAYRPYPYRADDGREHAPLDAGRVVRGASHDDPVDALRVTIRRLSSYDEGHGR
                     LAHGHHHTGFRCATSEDLGGR"
BASE COUNT          724 a         1904 c         1890 g          858 t
ORIGIN      
        1 aagacgcggc ggcagttcgg ggtgccgatc ggcagcttcc aggcgctgca gcaccgcatg
       61 gtggacatgg tgatcagcta cgagcaggcc cgctcgatgg cgtgcctggc gtgcgtgaag
      121 gtcgacagcg ccccgcccga cgagcggcgc cgggcggtct ccgcggcgaa gatcaaggtc
      181 gccgacgcct gccggcacgt gagccaggag gcggtgcagc tccacggcgg aatgggcatg
      241 accgaggagc tgaaggtgag ccacaccttc cggcggctca ccatgatcgg gcagacattc
      301 ggggacgccg agcaccacct cgagcgctac gccgtcgccg acggcgcggg ggcctgaccc
      361 gcgaaaagtt gacccccggc aggttcttgc ccatagtcga ttcgtatgga cgggcaggaa
      421 cccgcgggcc ggccgccgtc gttcttccgc aagcccggac cgcccggcgc gccgccgccc
      481 cgctccccga tgcctcccgc cgcgccgccg ccgaggcctc cgtcaccccc gtcggtctac
      541 gtgtcgccgc cgtccgggcc cggacccgag ccgcctgcgg tctcgaccgt tcggcgtccg
      601 accttccacg gcaacggcgg caccctgttc ggcattcacg tcgtcaacgt cctgctgacc
      661 atcgtcacgt tggggatcta ctacttctgg gcgaagacgc gcatccgctc gtatctctcc
      721 gggcagaccg agatcgaggg cgaccgcttc gcctatcacg gcacggcccg cgagctgctg
      781 ctgggcacga tcaaggcggt gctggtcttc ggcctgccga tcctcttcct gaacatgatc
      841 cgcgacgtcc tggacgtgcc gcccgtggcg aaggtgatcg cgggcgtggt ctccggcagc
      901 ctgttcttcg tgttcttccc gatcgcgatg gtgggcgcgc ggcgctatcg cttgagccgc
      961 acctcctggc ggggcatccg gttctccttc cgcgggcacg tgtgggcgct gatcaagatc
     1021 ttcatcctcg gcagcttcct gaccggcctc accctggggc tctactaccc gttcttcctc
     1081 gtgtcgcgtc aggcgttcat ggtcgcgcac tcgtacttcg gcagcgagag gttcgacttc
     1141 aacgggcgcg ggcgcgagct cttcgggccc ttcctggtgg caatattgct gaccctgccg
     1201 acgctcggcc tgtgctgggt ctggtacgtg gcgttcaagc gccgctactt ctgggaccac
     1261 accaccttcg ggaccgcgcg cttctcgtgt agcgtgaccg gcggcgccct gctgggattg
     1321 tgggtggtaa atctcatact gctcgtcggc acgctgggca tcgcgtggcc gtgggtccgg
     1381 gtgcgcaaca tccacttcgc cttccggaac ctggccctgg tgggcgccct cgacctcgcg
     1441 cggatccagc agcaggcgca gcaggtcggg agcatcgggg aaggcctgtc cggcttcttc
     1501 gactccggct tcgacctcgc ctgaccctcc cggcccccgg tgcggaccga ctggcagggc
     1561 atctatctcg atgggcagac cccggcccgg catgccgcga ccgtgcgtct gatgcgtgag
     1621 gcgctcgaga tcagccccgc gggcggcgcg ccccgcctct ggccctaccg cgagctgcgc
     1681 cagacccagg gcttctacgc gggggaggag gtgcggatcg agcggggcgg cgacctgccc
     1741 gagacgctgc tgatcccgga cagcgcgttc ctcgagagcc tccacgaggt cgcgcccggc
     1801 ctgcgcctgc gcttccatga cccgcgacgg cgccgggcgc gggtgcgctg gaccatcgcg
     1861 gccgcggcgg ccgtgctcgc ggtcaccgcc gcgatctacg tgtggggcat cccggcgctg
     1921 gccgccctcg tcgcgccgcg cgtgccggtg gcctgggagg agagcgtggg ccggtcggcg
     1981 atcgcctaca tggccccgcc ggaccgccgc tgcggcgatc cgcgcctggg tgccgcgatg
     2041 gacgagatcg tgcgccgcct caccgcctcc gcgccgccgt caccctacac cctgcgggta
     2101 tacgtcgtga accgggcggt ggtgaacgcg cacgcgctgc ccggcggcaa cgtcgtcatc
     2161 ttcaagggcc ttctcgagcg cacccgcacg ccggaggagc tggccggggt ggtcgcccac
     2221 gagctccagc acgtgctgcg gcgtcacacc acgcgcgcgg tgatccagga cgcctccacc
     2281 gggctcttgc tgatggcgct gaccggcgac gtgaccggcc cgctcgccta cgggctgcag
     2341 accgcgcgca cgctgggcga gctgcgctac tcgcggcgcg cggaggacga ggcggacacc
     2401 gaggggatga agatgctgct ggccgcccgg atcgatccgg cggggatgat cgacttcttc
     2461 gacaccatcg ggcaggagga gggccggcag gcgaaggcgc tgacctactt ctcgacccac
     2521 ccgctcgccg ccgaccggat cgcccgcctc aaggccatgg ccgcggcgtg gaagggcaca
     2581 cccgagcccc tcctccccgg cgaggactgg gcggccctcg ccaagcgctg ctgagggggc
     2641 ggccgctact tcggcttcca ggccttgaag cggtcgcgga gaaccttctt gtcgaacttg
     2701 cccacgctgg tcttgggcac cgcctcgatg aagacgacct cgtcgggcag ccaccacttg
     2761 gccacccggg gcttgaggta ctcgtagatc tcgtccgcgg tgagcgagcc cttgtgctcg
     2821 ggcttgggca cgatgcaggc gagcggccgc tccacccagc gctcgtgcgg caccgcgatc
     2881 accgccgcct ccagcacctt ggggtggccc atgatggtgg tctccatgtc cacgctcgag
     2941 atccactcgc cgcccgactt gatcaggtcc ttggtgcggt cggtgatctg gatgtagccg
     3001 tcgggatcca gcgcggccac gtcgccggtg cggaaccagc cgtcgccggt gaactgggct
     3061 gcgccctcgg ggttgttgta gtacccgctg gtgacccacg gcccacgcac ctggatctcg
     3121 cccgcgctat gcccgtccca cggcaggatg ttcccggcgt cgtcgacgat cctcatgtcg
     3181 acgccggcca ccggcgcccc ctgcttggcc cgcaccgcgt agcgctcgtc ctcggggagc
     3241 gcatccatgt aggagcgcgg ccggcagacg ctgccgagcg gggtcatctc ggtcatcccc
     3301 cacgcgtgga gcatgtaggc cccgtacttc ttgtcgaaca gctcgatgag ggtccgcggc
     3361 gcggccgatc cgccgatcgg gatgcagcgg atcgaggaga tgtcccactg cggctctttc
     3421 tcgagaatgc cctggatggc gatccagacc gtcggcaccg cgcccaccac cgtgaccttc
     3481 tcgtggtgga cgatctcgat gatgtcgcgc ggctgggggt tggggccgcc gaagatctgg
     3541 gtcgccccgt tcatgactcc cgcgaacggc acgcaccacg cgttggcgtg gaacatgggc
     3601 acgatgtgca ggatcacgtc gcgctcgcag atgccgagca cgtccaccgt ggaggacgcg
     3661 aagcagtgga ggtagatcgc gcggtgggtg tagaccacgc ccttcgggtg cccggtggtg
     3721 ccggaggtgt agcagatgcc ggccgcgtcc gtctcctcga ggcgcggcca ctcggtgacc
     3781 gggtcggtgc ccgccacgag ctgctcgtag tcgagcgcgc ccgggggcac gtgggcggtg
     3841 ggggtgtcct tcatgatgat gatgtgcttg acggtggtga ggtcgttccg gatcggctcg
     3901 aggatggacc agaggctcgc gtcgacgatc aggacctcgt cgcccgcgtg gttgacgatg
     3961 taggtgatgt cctgcgcgga gagccggatg ttcacggtgt ggagcaccat gcccatcagc
     4021 ggtgccgcga agtagacctc catgtggcgg tgggagttcc acgcgaaggt gccgacccgc
     4081 gtccccttct gcaggccgag gccgcgcagc gcgccggcca ggcggcagac ccggtccgcg
     4141 tagtccgcgt aggagtacca ctggagcccc atcccgggca cccgcgtcgc catgcgcttg
     4201 cggtggaaga gccgccgcgt gcgctcgaag aactgggtga gggtgagggg gtagtccatc
     4261 atcaggcctc tcatgacgtc atcgtcctcc caggtcctcc gaggtggcgc agcgaaagcc
     4321 ggtgtggtgg tggccgtggg ccagcctacc atgcccttca tcatacgagg agaggcgccg
     4381 gatcgtcacc cgcagtgcgt ccaccgggtc gtcgtggctc gcgccccgga ccactcgtcc
     4441 ggcgtcgagt ggggcgtgct cgcgcccgtc gtcggcccgg tagggatagg gccggtaggc
     4501 cgagcccgtc cactcccgca ggttcccgag caggtcctgg accccggtgg gcccggcccc
     4561 ggcgggccgg aggtcgccgc gctcggtggc gttgtagggg cgcccgacga tcgcccgctc
     4621 ggcatccggc gcctggtcgc cccacgggta gcggtggcgg tcgagcccgc gggccgcctt
     4681 ctcccactcg gcctcggtgg gcaggcgccg gccgagccag aggcagtagt cgcgggcccc
     4741 gaaccaggag acctccaccg cggggtgctc ctcgaagccg ggatcggcga tgaaccggcc
     4801 ctcgcgccgg tggatccgcg cgtcgggatc gtcctgatcg aagtagggca tgccttccgg
     4861 gctccggagc ccgcgggcct cgaggaaccg ggcaacttcc cgattcgtca ccttgttgcg
     4921 gtcgagccag aagtcgcgca cgtacacgcg gtgcatgggc ccctcgtccg ggacggtcga
     4981 atcgctgccc atccagaacg cgccggcctc gatgagcatg ataccgtcgg cgcgcgcctc
     5041 gcctgagccg ggcccgcgcc cggacgcgag cggcccgatc agggcgaggg ccaaggccgc
     5101 gcccagtgtg aggccccgtc gcgtccacgc ggtcacgcgc ccacgatagc gtcccgcaat
     5161 ctttaccgcc atggcggatt cgggtatggt gagggcgcga tgaactcccg ctggagcgac
     5221 ggtgacgcgc agggcaagag cgatctcgat ctcctcgtct acgcctcgcg cctgatcggc
     5281 gccgagacca cgctcgtggt ctggggcggg ggcaacacct ccatcaagca ggacgagcgc
     5341 gaccatcgcg gccgccccac ccgcgtgctc cgggtc
//
LOCUS       DATMCV010000651         2238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_31993, whole genome shotgun sequence.
ACCESSION   DATMCV010000651 DATMCV010000000
VERSION     DATMCV010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31993"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..639)
                     /locus_tag="VNU03_14965"
     CDS             complement(16..639)
                     /locus_tag="VNU03_14965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP75820.1"
                     /translation="MTTTVDPSRERWNRRWAGERASAATTPSAFLIAETESLPPGRAL
                     DVACGAGRNAVWLSRRGWRVTGVDFSEVALRAARDLAAGAGVEIEWVEANAVTWAPPG
                     RAYDLVTVMYLQLPAHERRVALAHAASAVRPGGTLLVLGHDLLNLTEGSGGPTQADVL
                     FTPDDVVAEIGDLAIEKALRVRRAVDGGGAARDAIDALVRARRPAGG"
     gene            715..>2238
                     /locus_tag="VNU03_14970"
     CDS             715..>2238
                     /locus_tag="VNU03_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75821.1"
                     /translation="MIGPRGHDVAVLWSRPSRDAYAALAVSRGETYRFTDAWGRPRTW
                     LKGEHAYLAPAGASVLLARCNLKHDLVEEADLTDERLGAVRALLVPNARHLADRTIGR
                     IEAWLRAGDRRLIVTGKTNLPPALLGLARVAPRPVTGYTGWRWRPGSPFAGPAWEPLY
                     VSGYAGHEAQQVEPASGSRVLADLVELTGDLTDATTATATPLGPAIVLTARTAYVANQ
                     VLELVGGMLQAHLNVEPVRHWANPTHWGDTLLFFLRRLLLEVGGEALWQTRLRSFGTH
                     DGVLSFRHDVHGMRDYTFLDYQIENLIPATYDIEDPGFSTNISEDMAADWVARTSRHD
                     FIEPALHNDSSIGDPPTAIHGTGLHDHVRRAAERLGFPVYTCGRHAGGHMHPETLDAM
                     DYLYAHDEQILGTCTFCYYHMIEYGVRDPKVMVGGQIGGKPLTYVTDVKRTIATPGIW
                     FPFHPVVTTQEEWRPLRGWDRTHEFDAASELVETIFGGHSARVPGVDDHLENGVYSFQ
                     "
BASE COUNT          317 a          864 c          768 g          289 t
ORIGIN      
        1 taggccgcgg ccgcctcagc cgcccgcggg acgccgggcg cggaccagcg cgtcgatcgc
       61 gtcgcgggcc gccccgcccc cgtcgaccgc gcggcgcacc cgcagggcct tctcgatcgc
      121 gagatcgccg atctccgcga ccacgtcgtc gggtgtgaag agcacgtcgg cctgcgtggg
      181 cccgcccgag ccctcggtga ggttcagcag gtcgtggccc aagaccagca gcgtgccgcc
      241 gggccgcacc gcggaagcgg cgtgtgccag cgcgacccgg cgctcgtggg cgggcagctg
      301 caggtacatc acggtgacga ggtcgtaggc ccgtccgggc ggcgcccagg tcaccgcgtt
      361 cgcctcgacc cactcgatct cgacgccggc gccggcggcc aggtcgcggg cggcccgtag
      421 cgccacctcg gagaagtcca cgccggtgac gcgccagccc cgccgcgaca gccacaccgc
      481 gttgcgaccg gccccgcacg ccacgtcgag ggcgcggccc ggaggcaggg actcggtctc
      541 ggcgatcagg aacgcgctcg gcgtcgtggc cgcgctcgcg cgctcaccgg cccagcgccg
      601 gttccagcgc tcgcgtgagg gatcgacggt ggtggtcatg gcgcgaatat accgcgggcg
      661 gcacgagcag acgaggctcg acaaggcgcg cgtccgcccg atagactgcc cggcgtgatc
      721 ggtccccggg ggcacgacgt cgcggtgctg tggagccggc cgagccgcga cgcctacgcg
      781 gccctggccg tgtcgcgggg cgagacgtac cggttcaccg acgcgtgggg gcgcccccgc
      841 acctggctca agggcgagca cgcctacctg gcccccgccg gcgcctccgt gctgctcgcc
      901 cgctgcaacc tcaagcacga tctcgtggag gaggccgatc tcaccgatga gcggctcggc
      961 gccgtgcgcg cgctgctcgt gcccaacgcg cggcatctgg ccgaccggac catcgggcgc
     1021 atcgaggcgt ggcttcgcgc cggcgatcga cgtctcatcg tcaccggcaa gaccaatctc
     1081 ccacccgcgc tcctcgggct cgcccgcgtc gcgccgcgcc cggtgaccgg ctacaccggc
     1141 tggcgctggc ggcccggatc gcccttcgcg ggcccggcgt gggagccgct ctacgtgagc
     1201 ggctacgccg ggcacgaggc gcagcaggtc gagcccgcct ccggcagccg cgtgctggcc
     1261 gacctggtcg agctgaccgg cgacctgacc gacgccacca ccgccaccgc aacgccgctc
     1321 ggccccgcca tcgtcctcac cgcgcgcacc gcctacgtgg ccaatcaggt cctcgagctg
     1381 gtgggcggca tgctccaggc gcacctcaac gtggagccgg tccggcactg ggccaacccg
     1441 acccactggg gcgacactct gctcttcttc ctgcgccgcc tgctgctcga ggtgggcggg
     1501 gaggcgctct ggcagacccg cctgcgctcg ttcggcactc acgacggtgt gctgtcgttc
     1561 cgccacgacg tgcacggcat gcgcgactac acgttcctcg actaccagat cgagaacctg
     1621 atcccggcca cctacgacat cgaggatccc ggcttctcga ccaacatctc cgaggacatg
     1681 gcggcggact gggtggcccg gacgagccgt cacgacttca tcgagcccgc gctccacaac
     1741 gacagctcga tcggcgatcc cccgaccgcc atccacggca ccggcctgca cgaccacgtg
     1801 cgccgggccg ccgagcggct cggcttcccg gtctacacct gcgggcgcca cgcgggcggg
     1861 cacatgcatc ccgagacgct cgacgcgatg gactacctct acgcccacga cgagcagatc
     1921 ctcgggacgt gcaccttctg ctactaccac atgatcgagt acggggtgcg agacccgaag
     1981 gtgatggtgg gcggccagat cggcggcaag cccctcacct acgtgaccga cgtgaagcgc
     2041 accatcgcca ccccgggcat ctggttcccc tttcatccgg ttgtcacaac ccaggaggag
     2101 tggcggccgc tgcggggctg ggaccgcacc cacgagttcg acgcggcgtc cgagctggtg
     2161 gagacgatct tcggcggcca cagcgcgcgg gtgcccggcg tcgacgacca cctggagaac
     2221 ggcgtctaca gcttccag
//
LOCUS       DATMCV010000652         3563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_233721, whole genome shotgun sequence.
ACCESSION   DATMCV010000652 DATMCV010000000
VERSION     DATMCV010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233721"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VNU03_14975"
     CDS             <1..989
                     /locus_tag="VNU03_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP75822.1"
                     /translation="SQAVAFKTLARRGWRPRGTLVYLACADEEAGGTLGAGHVCAQHW
                     EALDADYCLTENGGTVKAGPGGHGLNVTVHVGEKGVAWRRLRVKGTPGHGSMPYRADN
                     ALVKAAHVVSRLADYRPSPYVDELWRGFVGTLNLDPAVKEALVDPDRVDEAIAGLDPG
                     MAKYAWSATHTTFSPNVCGGGVKTNVIPDVVSVEVDIRTVPGDTEDEVRRHLDKAVGG
                     DLMDDIEIEKLFSKPASASPIGTPLWDVLGRVVDSHYPGARLMPRMIVGFTDAPYFRE
                     KGAIAYGFGLFSRALTAEAMAGRFHGNDERIDVESLALTTQAWLEVCEQFLG"
     gene            1012..3204
                     /locus_tag="VNU03_14980"
     CDS             1012..3204
                     /locus_tag="VNU03_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HWP75823.1"
                     /translation="MIPLTGYTDRLSAAAGERIAFKISSAAAGPYRASLARVVHADPN
                     PAGPGVKMVDLASRFAIERPSRIQPLALGSYARVDAAAALPTTGPLTVVALIWPTLAA
                     PGEQCVISRWDEGAGAGWALSVGPTGVTARVGVPGAAPFTLSSGRPATLRAWQRIWMV
                     IDPASGLLRVGQIALAGGEPLETRAPLPSAARPGAPAPILLGARLAGEACEHWNGKLE
                     DPLLLAAAAAAPESIALDPLLPPTGLLAGWDFSRGIDGLDVVDVGPHRLGGQLVNLPT
                     RAVTGARWTGNEMCWRHAPREYGAIHFHDDDLHDAGWATDFDFTVPADLPSGAYVMRL
                     AADGHADELPFYVRPPRGRPGADVLFIASTYTYQAYANHARSSTDAAYRARVAAWDAY
                     PHNPDDHPDYGRSTYNRHRDGSGICYSSRLRPVLTWRPRYLTFLDARGSGMRHYPADT
                     HLLDWLEAQGIAYDVVTDEDVEAEGAALLAPYATVLTGSHPEYHTARTLDAHAGYLAG
                     GGRLVYLGGNGFYWRIATSPAVPGVIEVRRAEGGIRAWEAQPGEYYHALDGQYGGLWR
                     RNGRPPQRLVTVGFSGQGLFEGSHYRRQAGADDPRAAFIMAGVKDAVIGDFGLSGGGA
                     AGFELDRADAALGTSPGAVVVASSEGHGPSYVVVPEELLSHLATVSGEKPARLLRADM
                     TYAELPGGGAVFAVGSITFCGSLSHNRYDNNVSRILRNVLDRFRARGA"
     gene            3204..>3563
                     /locus_tag="VNU03_14985"
     CDS             3204..>3563
                     /locus_tag="VNU03_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWP75824.1"
                     /translation="MLDVPRLRADTPGVTRVLHFNNAGSALPPRPVLEAVTGHLEREA
                     AIGGYEAAAEAADRVADFYPAVASLIGADPDEIAFVENATRAWDMAFYAVPFRAGDRV
                     VTARAEYASNYLAFLQLR"
BASE COUNT          450 a         1356 c         1283 g          474 t
ORIGIN      
        1 cctcccaggc ggtggccttc aagaccctgg cgcgccgcgg atggcggccg cggggcacgc
       61 tggtctacct ggcctgcgcg gacgaggagg ccggcggcac cctcggggcc gggcacgtgt
      121 gcgcacagca ctgggaggcg ctcgacgctg actactgcct gaccgagaac ggcggcaccg
      181 tcaaagcggg cccgggcggc cacgggctca acgtcacggt gcacgtgggc gagaagggcg
      241 tggcctggcg gcggctccgt gtgaagggca cgccgggaca cggctcgatg ccctaccggg
      301 cggacaacgc gctggtcaag gccgcgcacg tggtgagccg cctcgccgac taccgtccgt
      361 cgccctacgt ggacgagctc tggcgcggct tcgtgggcac gctgaacctc gacccggcgg
      421 tgaaggaggc cctggtcgac ccggaccgcg tcgacgaggc gatcgccggc ctcgaccccg
      481 gcatggcgaa gtacgcgtgg tcggccaccc acaccacctt ctcgcccaac gtgtgcgggg
      541 gcggcgtgaa gaccaacgtg atcccggacg tggtcagcgt cgaggtcgac atccgcaccg
      601 tgcccgggga caccgaggac gaggtgcggc gccacctcga caaggcggtg ggcggtgacc
      661 tgatggacga catcgagatc gagaagctct tctccaagcc cgcgtcggcc tcgcccatcg
      721 ggacgccgct ctgggacgtg ctcggccgcg tcgtcgactc gcactacccg ggggcgcgcc
      781 tcatgccccg catgatcgtc ggcttcaccg acgcgcccta cttccgcgag aagggcgcga
      841 tcgcctacgg cttcggcctg ttctcccggg ccctgaccgc cgaggcgatg gcgggccggt
      901 tccacggcaa cgacgagcgc atcgacgtgg agtcgctggc gctgaccacg caggcctggc
      961 tcgaggtgtg cgagcagttc ctcggatagg ccgcgaccgg gcccgctccc agtgatcccg
     1021 ctcaccggct acaccgaccg cctctccgcc gccgccggtg agcgcatcgc gttcaagatc
     1081 tcgagcgccg cggccggccc gtatcgcgcg agcctcgccc gggtcgtgca cgcggacccg
     1141 aacccggccg gacccggcgt gaagatggtg gacctggcgt cccgcttcgc gatcgagcgc
     1201 ccctcgcgga tccagccgct cgccctgggc tcgtacgcgc gcgtcgatgc cgcggcggcg
     1261 ctgcccacca ccggccctct caccgtggtc gcgttgatct ggccgacgct cgcggcgccg
     1321 ggcgagcagt gcgtgatctc ccgctgggac gagggtgccg gcgcgggatg ggcgctctcg
     1381 gtgggcccga ccggggtgac cgcgcgcgtc ggcgtgcccg gcgcggcgcc gttcaccctt
     1441 tcgagcggcc ggcccgccac gctgcgagcg tggcagagga tctggatggt gatcgacccg
     1501 gcgagcgggc tcctgcgcgt cggccagatc gcgctggccg gcggcgagcc gctcgagacc
     1561 cgggcgccgc tgccgtccgc ggcccggccc ggcgcgcccg cgccgatcct gcttggcgcc
     1621 cgcctggccg gcgaggcgtg cgagcactgg aacggcaagc tcgaggaccc gctcctgctc
     1681 gcggccgcgg cggcggcacc ggagtcgatc gccctcgatc ccctgctccc gcccaccggg
     1741 ctcctcgcgg gctgggactt ctcccgcggc attgacggcc tcgacgtcgt cgacgtgggg
     1801 ccgcatcgcc tcggcgggca gctggtgaac ctgcccaccc gcgcggtcac cggcgcgcgc
     1861 tggaccggca acgagatgtg ctggcggcac gccccgcgcg agtacggggc catccacttc
     1921 cacgacgacg atcttcacga cgcgggatgg gccaccgact tcgacttcac ggtgccggcg
     1981 gacctgccga gcggcgccta cgtcatgcgc ctggccgccg acggccacgc cgacgagctg
     2041 cccttctacg tgcggccgcc tcgcggccgg cccggcgcgg acgtgctctt catcgcctcg
     2101 acctacacct accaggccta cgccaatcac gcgcggagct cgaccgacgc ggcctatcgc
     2161 gcgcgggtcg ccgcgtggga cgcctacccg cacaatccgg acgaccatcc ggactacggc
     2221 cggtcgacct acaaccgcca ccgcgacggc agcggcatct gctactcctc gcgcctgcgt
     2281 ccggtgctga cctggaggcc gcgctacctc accttcctcg acgcgcgcgg ctcgggcatg
     2341 cgtcactacc cggccgacac gcacctgctc gactggctcg aggcccaggg catcgcgtac
     2401 gacgtggtca ccgacgagga cgtcgaggcc gagggcgcgg ccctcctggc cccgtatgcc
     2461 accgtgctca ccggctcgca ccccgagtac cacaccgcgc gcaccctcga cgcccacgcg
     2521 ggctatctgg ccggcggcgg gcgcctcgtc tacctgggcg gcaacggctt ctactggcgc
     2581 atcgcgacca gccccgcggt gccgggcgtg atcgaggtgc gccgcgccga gggcgggatc
     2641 cgcgcgtggg aggcccagcc cggcgagtac taccacgcgc tggacgggca gtacggcggg
     2701 ctctggcggc gcaacggacg gccgccgcag cggctcgtga cggtcggctt ctcgggtcag
     2761 ggcctcttcg agggctcgca ctaccgacgc caggccggcg ccgacgaccc gcgcgcggcc
     2821 ttcatcatgg cgggcgtcaa ggacgcggtc atcggcgact tcgggctctc gggtggcggg
     2881 gccgccggct tcgagctgga ccgcgcggac gcggcgctgg ggacgtcgcc cggcgcggtg
     2941 gtggtggcct cgtccgaggg ccacgggccg agctacgtgg tggtgccgga ggagctgctc
     3001 tcgcacctgg cgacggtgag cggcgagaag ccggcccggc tgctgcgcgc ggacatgacc
     3061 tacgccgagc tgccgggcgg cggcgcggtc ttcgcggtgg ggtccatcac cttctgcggg
     3121 agcctgtcgc acaaccgcta cgacaacaac gtctcgcgca tcctgcgcaa cgtcctcgac
     3181 cgcttccgcg cccggggcgc ctgatgctcg acgtcccccg gctgcgcgcc gacaccccgg
     3241 gcgtcacccg cgtgctgcac ttcaacaatg cgggctcggc cctgccgcca cggccggtgc
     3301 tcgaggcggt gaccggccac ctggagcgcg aggccgccat cggcggctac gaggccgccg
     3361 cggaggccgc ggatcgcgtg gccgacttct atcccgcggt ggcctccctg atcggcgccg
     3421 atcccgacga gatcgccttc gtggagaacg ccacccgggc gtgggacatg gccttctacg
     3481 cggtgccgtt ccgggccggc gaccgggtgg tcaccgcccg ggccgagtac gcgtcgaact
     3541 acctcgcgtt cctgcagctc cgg
//
LOCUS       DATMCV010000653         3208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_567898, whole genome shotgun sequence.
ACCESSION   DATMCV010000653 DATMCV010000000
VERSION     DATMCV010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567898"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="VNU03_14990"
     CDS             <1..1508
                     /locus_tag="VNU03_14990"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HWP75825.1"
                     /translation="ADRARPSEEGAMGARSGEQFLQGLRSTKRHLWLEGERVDDVTAH
                     PALAGGARTLAGIFDRLHNFPDECLIADPETGEPLNIGHMIPRSIDDLKRRNRGLARI
                     AEATVGLMGRTPDYMNVKFASFAGRWRDWLGADGRNEEGARNLVAFQKRLAREDISLT
                     HTIIHPTIDKSTDAQIVGNRVPVHRTATTSGGIVVRGARILATLAPFADEIAVYPAHP
                     LPAGAEAYALAFSIPVDTPGLIFMCRDSASAPGAVFDRPLSTRFDEQDAFVIFDDVEV
                     PRERLFIDGQIDVYNTLPLTGLRDNMTNQTTIRALTKLEFAYGLATRMADAIGDASPT
                     THEMLGELLDYAEVARSAVLLSAEHGRDVGDGVWFPDGRPLAPMRSLLATWFPRVNEI
                     IMLIGSHNLLAAPSRRQLDDATLRPLIDEFLHGAKGTGAEERAALFRLAWDFVGSTLA
                     GRNVLYERFYLTSAARNRINHHLRNTDRARAYALVDGILAAGRGAGGLADA"
     gene            1528..2652
                     /locus_tag="VNU03_14995"
     CDS             1528..2652
                     /locus_tag="VNU03_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWP75826.1"
                     /translation="MQTFVPMVGGLVLALLMTTAGAAQAATFVYVSNAEDGDIGAYAL
                     LPDGSLVPGARVAAGKPVMPMSVSPDRRFLYAAVRSKPFAVITYAIDPKTGALTRRSD
                     AALAESLPYIRVDKTGTVMLGASYGGHLVSVNTIGKDGVVSDPKQVIPTARNAHSIIT
                     DNTNRFVFVPHLGTDQIFQFRLDAKAGRLTANTPPVVQMKAGSGPRHIIMSSDNRFAY
                     LLNELTATVTTLALDARTGLLTEVGSASALPPDTKLVPGAPRPSPGRNVDNDIWASDL
                     HLTPDGKLLYAAERTSSSLGALRVDPATGTLTYLSSTPTEPQPRGFAIDPTGRTMIVS
                     GEKSDTLSVYAIDPVSGALSLRQKYPTGKGSNWVEIVSFD"
     gene            complement(2669..3115)
                     /locus_tag="VNU03_15000"
     CDS             complement(2669..3115)
                     /locus_tag="VNU03_15000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWP75827.1"
                     /translation="MKKRSGPFWDGIEGRAPMPRAAASLGLQFIAADPEKGTIELAFA
                     GREDFTNPAGNVLGAFVAAMLYDTVGPALLATLEPDQFQSTTQFNVNFLRPVRPGRVV
                     GKGRVVHRDGDIAFLEASLFDSRGALIATATATARVIPLERAGSAA"
BASE COUNT          491 a         1223 c         1044 g          450 t
ORIGIN      
        1 gggccgaccg ggcgcgtccg agcgaggagg gggccatggg ggcacgatcc ggcgagcagt
       61 ttctccaggg gctgcgcagc accaagcggc atctctggct cgagggcgag cgggtggacg
      121 acgtcaccgc gcaccccgcg ctcgcgggcg gggcccggac gctggccggg atcttcgatc
      181 ggctccacaa tttccccgac gagtgcctga tcgcggatcc ggagaccggc gagccgctca
      241 atatcgggca catgatcccg cgctcgatcg acgatctgaa gcggcgcaac cggggtctcg
      301 cgcggatcgc ggaggccacc gtcggcctca tgggccgcac ccccgattac atgaacgtca
      361 agttcgcgag cttcgcgggg cgctggcggg actggctcgg cgcggacggg cgcaacgagg
      421 agggggcgcg caacctggtc gcgttccaga agcggctcgc ccgcgaggac atctcgctca
      481 cccacacgat catccacccg accatcgaca agtcgacgga cgcgcagatc gtggggaacc
      541 gcgtgcccgt ccaccggacc gccacgacct cgggcggcat cgtcgtgcgc ggcgcccgca
      601 tcctggcgac cctggcgccc ttcgccgacg agatcgcggt ctatcccgcg caccccctgc
      661 ccgcgggggc cgaggcctac gcgctcgcct tctcgatacc ggtggacacg cccgggctca
      721 tcttcatgtg ccgcgacagc gcatccgcgc ctggcgcggt gttcgaccgg ccgctctcga
      781 cccgcttcga cgagcaggac gccttcgtga tcttcgacga cgtcgaggtt ccgcgggagc
      841 ggctcttcat cgacggccag atcgacgtct acaacaccct ccccctcacc ggcctgcgcg
      901 acaacatgac gaaccagacc accatccgcg cgctcaccaa gctcgagttc gcctacgggc
      961 tcgcgacccg catggcggac gcgatcggcg acgcgagccc gacgacccac gagatgctgg
     1021 gggagctgct cgactacgcc gaggtcgccc gcagcgcggt cctcctctcc gccgagcacg
     1081 ggcgagacgt gggagacggg gtgtggttcc ccgacggccg ccccctcgcg ccgatgcggt
     1141 cgctgctcgc cacgtggttc ccgcgcgtca acgagatcat catgctcatc ggcagccaca
     1201 acctcctggc cgcgccgagc cgccgccagc tcgacgacgc cacgctccgg ccgctgatcg
     1261 acgagtttct ccacggcgcg aagggcaccg gggccgagga gcgggccgcc ctgttccgcc
     1321 tcgcctggga cttcgtcggc tcgaccctcg cgggccgcaa cgtcctttac gagcgcttct
     1381 atctcacctc cgcggcgcgc aaccgcatca accaccatct ccggaacacc gaccgggccc
     1441 gcgcctatgc cctcgtcgac gggatcctgg ccgccggccg aggggcgggc ggactcgccg
     1501 acgcgtgacc ctcgagagga gaccctcatg cagaccttcg tccccatggt gggcgggctc
     1561 gtgctcgccc tgctcatgac cacggccggc gcggcccagg ccgccacgtt cgtctacgtg
     1621 tcgaacgccg aggacggcga catcggcgcc tacgccctgc tccccgacgg cagcctcgtg
     1681 cccggggcgc gcgtcgccgc cggcaagccg gtgatgccga tgagcgtcag ccccgaccgg
     1741 cgctttctct acgccgcggt gcggtcgaag cccttcgcgg tcatcaccta cgccatcgat
     1801 cccaagaccg gagcgctgac ccggcgatcg gacgcggcgc tggcggagag cttgccctac
     1861 atcagggtgg acaagaccgg aaccgtcatg ctcggcgcct cctatggcgg ccacctcgtg
     1921 agcgtcaaca cgatcggcaa ggacggcgtg gtgtccgacc ccaagcaggt gatcccgacc
     1981 gcccgcaacg cccactccat catcaccgac aacaccaacc ggttcgtgtt cgtgccgcac
     2041 ctcggcaccg accagatctt ccagttccgt ctcgacgcaa aggccggccg gctgaccgcg
     2101 aacaccccgc cggtggtcca gatgaaggcc gggtccggcc cgcgccacat catcatgtcg
     2161 agcgacaacc gcttcgccta cctcctcaac gagctgaccg cgaccgtgac gacgctcgcg
     2221 ctggacgcca ggaccggcct gctgaccgag gtgggctcgg cctcggccct gccgcccgac
     2281 accaagctcg ttcccggcgc cccgcgtccc tcgcccgggc gcaacgtcga caacgacatc
     2341 tgggcgtcgg acctgcacct cacgccggac ggcaagctcc tctacgccgc cgagcggacc
     2401 agcagctcgc tcggcgcgct ccgggtggac ccggccaccg gcaccctcac gtatctctcg
     2461 agcacgccga ccgagcccca gccgcgcggc ttcgccatcg atcccaccgg ccgcaccatg
     2521 atcgtgtccg gcgagaaatc ggacaccctc tcggtctacg ccatcgaccc ggtgagcggc
     2581 gccctgtcgc tgcggcagaa gtaccccacc ggcaagggct cgaactgggt cgagatcgtg
     2641 agcttcgact agcgggagat ggcgacccct aggccgcgga tccggcccgt tcgaggggga
     2701 tgacccgcgc ggtcgcggtg gcggtggcga tgagggctcc cctcgaatcg aacagcgagg
     2761 cttccaggaa cgcgatgtcg ccgtctcggt gaacgacacg ccccttgccg acgacccgtc
     2821 cgggccggac cgggcgcagg aagttgacgt tgaactgcgt ggtcgactgg aactggtccg
     2881 gctcgagggt cgccagcagc gccggcccca ccgtgtcgta gagcatcgcg gcgacgaacg
     2941 cgccgagcac gttgccggcc gggttggtga agtcctcccg ccccgcgaac gcgagctcga
     3001 tcgtcccctt ctcgggatcc gcggcgatga actgaaggcc cagcgaggcc gcggcgcgcg
     3061 gcatcggcgc tcgtccttcg atcccatccc agaacggccc tgatcgcttc ttcatcgcat
     3121 ctctcctggc gtgtcgtcgt cggcactcgt cacttcgccg cgcggggcgg cggcgcgagc
     3181 ggtgacatgc ggtcgagtgt ccgcgcca
//
LOCUS       DATMCV010000654         4669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_831567, whole genome shotgun sequence.
ACCESSION   DATMCV010000654 DATMCV010000000
VERSION     DATMCV010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831567"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VNU03_15005"
     CDS             <1..328
                     /locus_tag="VNU03_15005"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWP75828.1"
                     /translation="ADAARMLRDLRGREHQVITGLAVVETGPPGTAPRQETASVTSRV
                     RMGGYGDAEIDAYVATGEPLDKAGGYAVQGLGGRLVEAVDGCFTNVVGLPLTTTRALL
                     ARRGLL"
     gene            complement(325..1275)
                     /locus_tag="VNU03_15010"
     CDS             complement(325..1275)
                     /locus_tag="VNU03_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75829.1"
                     /translation="MIAWLVLLQASLSIAVGGPITSPEYLPLHVAQAQGYFAAQKLDV
                     SLRTVRSEAGAAEALGRGQVQLAATSLDAALRLGHSEGIPPRIVWGLTGAAPVALLVN
                     PARAESIRSAADLVGQTVAVPAPGTAEDQALGLLLVRAGVPFGRVTLRSLGERGTARA
                     LEGGAVAAAVLAEPFVSRLVADGKAKVVVDLRTAAAAADALGGPTVNAALFARAGAPP
                     DAATLRALGQALQAAIARVIGSEPAALAEGLPPAVIGTPADFAARLQSARDLFLPGGR
                     VSVEALEHSLELARARGPLPIQVKVPKRVEQLLMEPPGGG"
     gene            complement(1286..2404)
                     /gene="murG"
                     /locus_tag="VNU03_15015"
     CDS             complement(1286..2404)
                     /gene="murG"
                     /locus_tag="VNU03_15015"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWP75830.1"
                     /translation="MLVVISGGGTGGHTSPGLAVAARLREASVDVHWIGSRAGIEARR
                     VPEAGITFHSIPVGKLRRYWDWQNVPDLAVRAPAGLAQAWRLLRRLRPALLLATGGFV
                     ALPPALAARALRIPVVVHEQTSVPGLANRVAGRFARRIALTFPLTGNELPQERTVLTG
                     NPLRPELRGGSRAAACRRFGLEDAGPIVYVTGGALGSHRLNRDVGEALPRLLERCQVI
                     HQCGDNKATGDLAWLGERARALPEGLRRRYSLQAYVGAELRDVYAATALVIGRSGAGT
                     VNECCQLGLPALYVPLPGTSGDEQTANARLVEAAGGAVVFPQVSLTSGGLVEAVERLL
                     GDPAALAAMGERARSLAVPDAADRIVRLLFEVVGANPR"
     gene            complement(2548..3666)
                     /locus_tag="VNU03_15020"
     CDS             complement(2548..3666)
                     /locus_tag="VNU03_15020"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HWP75831.1"
                     /translation="MAKTPRKLRVGVVFGGRSGEHEVSLAGAASVMAAMDRSRFDLTP
                     IGIAKDGRWLMGGDPLRALAQEAARQALTEGGPDASTKHELLERAAATDTGAALMRME
                     TTEGLPPGLRERLDVVLVLLHGPQGEDGTIQGLLQLAGVPYTGAGVLASAVGMDKVAM
                     KDLFRAHNLPIVEYATVRRHDWEREPDPIARGIAGEIGFPCFVKPANLGSSVGISKVK
                     APEDLAAAIDLAARYDRRIIVERGIQGREVEVAVLGNDEPQVSVPGEVTFPGEWYDYD
                     TKYGAGQTTLKIPAPLPPEVTEEVRALALRAFRATDCAGMARVDFFIEGDHRVLLNEI
                     NTIPGFTSTSAYPRLWEASGIPYPELISRLIALALERR"
     gene            complement(3736..>4669)
                     /locus_tag="VNU03_15025"
     CDS             complement(3736..>4669)
                     /locus_tag="VNU03_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWP75832.1"
                     /translation="PYVEKGTIILVGATTENPSFEVNGALLSRARVYVLRPLGEAELV
                     TILERALADPERGLGRLGASVEPEALALIARLSDGDARTALNVLELAVSEPGARLTQA
                     AVREAAQKKALLYDKSGEQHYNLISALHKSLRDSDPDGALYWLGRMLEAGEDPLYVAR
                     RLVRFASEDVGNADPAALRLTLDAKDAYHFLGTPEGELALAQATCYLALAPKSNAVYA
                     AWNAVRRDIEEQPAEPVPLHLRNAPTGLMGNLGYGRGYQYAHDAPDAQVSQEHLPDVL
                     RGRVYYQPTDRGAEKEIADRLAKWRAWRDRQKRG"
BASE COUNT          592 a         1689 c         1784 g          604 t
ORIGIN      
        1 cgccgacgcg gcccgcatgc tccgggacct gcgggggcgc gagcaccagg tgatcaccgg
       61 cctcgcggtg gtggagacgg gcccgcctgg cacggcgccg cgacaggaga ccgcctcggt
      121 cacgagccgg gtccggatgg gcggctacgg agacgccgag atcgacgcct acgtggcgac
      181 cggcgagccg ctcgacaagg cagggggcta cgcggtgcag gggctgggtg gccggctggt
      241 ggaggcggtc gacggctgtt tcaccaacgt ggtgggcctg ccgctcacca ccacgcgtgc
      301 cctgctcgcc cgccgggggc tcctctagcc cccgcccggc ggctccatca ggagctgctc
      361 cacccgcttc gggaccttca cctggatggg caacgggccg cgggcccggg ccagctcgag
      421 actgtgctcg agcgcctcga cgctcacccg cccaccgggc aagaagaggt cgcgcgcgct
      481 ctgcaggcgc gccgcgaagt cggccggcgt gccgatcacc gcgggcggca gtccctcggc
      541 gagcgcggcc ggctcgctcc cgatcacgcg ggcgatggcg gcctgcaggg cctggccgag
      601 cgcccgcagc gtggccgcgt ccggcggcgc gccggcgcgc gcgaagagcg ccgcgttcac
      661 cgtcgggccc ccgagcgcgt cggccgccgc cgcggcggtg cggaggtcca ccaccacctt
      721 cgccttgccg tcggccacca gccggctcac gaagggctcg gccagcaccg cggccgccac
      781 cgcgccgcct tcgagcgccc gggccgtgcc gcgctcgccc aggctgcgga gggtgacccg
      841 gccgaacggc acgcccgcgc gcacgagcag gaggccgagc gcctggtcct cggcggtgcc
      901 gggcgcgggc accgcgacgg tctgccccac cagatcggcg gcggagcgga tggactcggc
      961 ccgcgccgga ttgacgagca gggccaccgg cgccgccccg gtgagccccc acacgatgcg
     1021 cggcgggatt ccctcggagt ggccgagccg gagcgcggcg tcgagcgagg tggcggcgag
     1081 ctggacctgg ccccggccga gcgcctccgc ggcgcccgcc tccgagcgca cggtccgcag
     1141 ggagacgtcg agcttctggg ccgcgaagta gccctgagcc tgggcgacgt ggagcggcag
     1201 gtattcgggt gacgtgatcg gtccgcccac cgcgatgctg agcgacgcct gcaggagcac
     1261 gagccaggcg atcatgggcg ggcgctcatc gcgggttggc gcccacgacc tcgaagagga
     1321 gccggacgat gcgatcggcc gcgtcgggca ctgccaggct ccgggcccgc tcgcccatcg
     1381 cggcgagggc cgcgggatcc cccaggaggc gctccacggc ctccacgagg cctccagacg
     1441 tgagcgagac ctgcgggaac accaccgcac ctcccgcggc ctccacgagg cgcgcgttgg
     1501 ccgtctgctc gtctccgctc gttccgggca gcggcacgta gagggcgggg aggccgagct
     1561 ggcagcactc gttgaccgtc cccgcgccgc tgcgcccgat gacgagggcg gtggcggcgt
     1621 agacgtcgcg tagctcggct cccacgtagg cctggagcga gtagcggcgt cgcaggccct
     1681 cgggcagcgc gcgggcccgc tcgccgagcc acgccaggtc gccggtggcc ttgttgtcgc
     1741 cgcactggtg gatgacctgg cagcgctcca gcaggcgcgg gagcgcctcg ccgacgtcgc
     1801 ggttgagccg gtgcgagccc agcgctccgc cggtgacgta cacgatcggc cccgcgtcct
     1861 cgaggccgaa gcgccggcac gccgcggccc gcgagccgcc gcgcagctcc gggcggagcg
     1921 ggttgccggt gaggacggtg cgctcctgcg gcagctcgtt cccggtcagc gggaaggtca
     1981 gcgcgatgcg gcgggcgaag cggccagcca cgcgattggc gaggccgggc acgctggtct
     2041 gctcgtggac gaccaccggg atgcgcagcg cgcgggcggc cagggcgggc ggcagcgcga
     2101 cgaagccgcc ggtggccagg agcagcgccg gccgcaggcg ccggagcagc cgccaggcct
     2161 gggcgagacc ggccggcgcc cgcaccgcga ggtccggcac gttctgccag tcccagtagc
     2221 ggcgcagctt gcccaccggg atcgaatgaa aggtgatgcc ggcctcgggc acgcggcgcg
     2281 cctcgatgcc ggcgcggctg ccgatccagt gcacgtccac cgaggcctcg cgcagccgcg
     2341 cggcgacggc caggccgggg ctcgtgtgtc cgccggtgcc tccgcctgaa attacgacta
     2401 gcatgcggcg actagcatgc ggcggctagc atttggtttc ggcgctagga ctgggtaatt
     2461 gttttatacg ggggcccaga aatggccccc gtactccccc aggctgcggg cgaccgtgtg
     2521 cgggcgatgc ttggcgcggc actcgtccta ccggcgctcg agggcgaggg cgatgaggcg
     2581 ggagatcagc tcggggtagg ggatgccgga ggcttcccag agccgcgggt acgcgctcgt
     2641 cgaggtgaag ccggggatgg tgttgatctc gttcaggagc acgcggtggt cgccctcgat
     2701 gaagaagtcg acccgcgcca tccccgcgca atcggtggcc cggaaggccc ggagcgcgag
     2761 cgcccgcacc tcctcggtca cctcgggcgg cagcggcgcg gggatcttga gcgtggtctg
     2821 cccggcgccg tacttggtgt cgtagtcgta ccactcgccg gggaaggtga cctcgccggg
     2881 caccgagacc tggggctcgt cgttgcccag caccgccacc tccacctcgc ggccctggat
     2941 cccgcgctcc acgatgatcc gccgatcgta ccgcgcggcc aggtcgatgg cggcggcgag
     3001 atcctcggga gccttgacct tgctgatgcc gacgctcgag cccaggttgg ccggcttgac
     3061 gaagcagggg aagccgatct cgccggcgat gccgcgggcg atcgggtcgg gctcccgctc
     3121 ccagtcgtgc cgcctcacgg tggcgtactc gacgatcgga agattgtggg cgcggaagag
     3181 gtccttcatc gcgaccttgt ccatgccgac cgcggaggcg agcacgcccg cgccggtgta
     3241 ggggacgccc gccagctgca gcaggccctg gatcgtcccg tcctcgccct gcgggccgtg
     3301 caggaggacc aggacgacgt cgaggcgctc gcgcaggccc ggcggcagcc cctcggtggt
     3361 ctccatgcgc atgagcgcgg cgccggtgtc ggtggcggcc gcccgctcga gcagctcgtg
     3421 cttggtggag gcgtccgggc cgccctcggt cagggcctgg cgcgccgcct cctgggccag
     3481 cgcgcgcagc gggtcgccgc ccatgagcca gcggccgtcc ttggcgatgc cgatgggggt
     3541 gagatcgaag cggctccggt ccatcgccgc catcaccgac gcggcgcccg cgagggagac
     3601 ctcgtgctcg cccgaccggc cgccgaacac cacgccgacc cgcagcttcc ggggcgtttt
     3661 cgccatgagt tcagttgtat cagaccacgg ttttccgggg cgagaactcc ttcgacccga
     3721 ccgcgagcgg accgctcacc cgcgcttctg ccggtcccgc cacgcccgcc acttggcgag
     3781 gcggtcggcg atctccttct cggcgccgcg atccgtcggc tggtagtaca cgcggccccg
     3841 caggacgtcg ggcaggtgct cctggctcac ctgggcgtcg ggggcgtcgt gcgcgtactg
     3901 gtagccgcga ccgtagccca ggttgcccat gaggccggtg ggcgcgttgc gcaggtggag
     3961 cggcaccggt tcggcgggct gctcctcgat gtcgcggcgg accgcgttcc atgccgcgta
     4021 caccgcgttg gatttcgggg cgagcgcgag gtagcacgtc gcctgggcga gagccagctc
     4081 gccctcgggg gtgccgagga agtgataggc gtccttggcg tcgagggtca ggcgcagcgc
     4141 ggccgggtcc gcgttgccca cgtcctccga ggcgaagcgg accaggcgcc gcgccacgta
     4201 gagcgggtcc tcgcccgcct ccagcatgcg gccgagccag tagagcgcgc cgtcggggtc
     4261 ggagtcgcgg agggacttgt ggagcgccga gatcaggttg tagtgctgct cgcccgactt
     4321 gtcgtagagc agcgccttct tctgggccgc ctcccgcacc gcggcctggg tcaggcgggc
     4381 gcccggctcg ctcaccgcga gctcgagcac gttgagcgcg gtgcgggcgt cgccgtcgga
     4441 gaggcgcgcg atcagcgcga gcgcctccgg ctccaccgag gcgccgagcc ggcccaaccc
     4501 gcgctcgggg tccgccagcg cgcgctcgag gatggtcacc agctcggcct cgcccagcgg
     4561 tcgcagcacg tagacccgcg cgcgcgacag gagcgcgccg ttgacctcga acgaggggtt
     4621 ctctgtggtg gctccgacca ggatgatcgt gcccttctcg acgtagggg
//
LOCUS       DATMCV010000655         2460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_302060, whole genome shotgun sequence.
ACCESSION   DATMCV010000655 DATMCV010000000
VERSION     DATMCV010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302060"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VNU03_15030"
     CDS             complement(<1..518)
                     /locus_tag="VNU03_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010196833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HWP75833.1"
                     /translation="MAVRVGVNGFGRIGRVFFRAALESSDIEVVAVNDLADAKTLAHL
                     LKHDSVHGTLDAEVKAKGEAIFVNGREIRVCSAKDPATLPWGEMGVGIVVESTGIFRD
                     RATSSKHLQAGAKKVVITAPAKDPDITVVLGVNEQKYDPVKHELVSNASCTTNCLATV
                     AKVLLDTFGIKRG"
     gene            complement(661..1479)
                     /locus_tag="VNU03_15035"
     CDS             complement(661..1479)
                     /locus_tag="VNU03_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HWP75834.1"
                     /translation="MPSLEHVPTLDRPRPDPALWPRWAGALLDLVFPPFCPVCRARLE
                     EGRRDPLCGACWAGLPRIGPPWCRVCGQPMAGFPVAAGPTTAAPTRAEGGALCAACRR
                     RPPAFAYARAALRYEDVAREALHAFKFGGHRALAGPLGDALVEAVEGRLPGGAPDLLV
                     PVPLHPRRERERGFNQSRLLARRLGRAWGCPVRDDVLVRAVATPSQTSLDGAARRANV
                     RRAFRLRRPELVAGRHVVLVDDILTTGATLSECARCLEDAAPRIVGVLAVARVV"
     gene            complement(1449..>2460)
                     /locus_tag="VNU03_15040"
     CDS             complement(1449..>2460)
                     /locus_tag="VNU03_15040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP75835.1"
                     /translation="GLVVGLLFAILVTYLASLNPGRAHLALGGDWAVDVPLMGLVVGV
                     FGAGALLALVLGWLRDLTRPYARGPVAVAAPRASLRPDTAPLRAAEPAPAPARAARDA
                     LIERGRFADALVAQTQILAGVPREERAAEEVTLAGLHYEVGRERLERGDLASAVSHFK
                     DALRMQPDFVPAALLLGEAHLKAGEPRDALRVWERAAEAPQPALPVLARIEQRHREEG
                     RPTRMISLYRNALDRHPDNLALAFGLGRVYFELAMLDEAAEQFQKMEVRAADLPLIHA
                     YLGAILERRGQFRDAMEEYRRALRLSDSVQWPHHCATCGALHPRWVDRCPSCRRWNTS
                     RP"
BASE COUNT          349 a          906 c          915 g          290 t
ORIGIN      
        1 ccgcgcttga tgccgaaggt gtcgagcagg accttggcga cggtggccag gcagttggtg
       61 gtgcaggaag cgttggacac cagctcgtgc ttgaccggat cgtacttctg ctcgttgacc
      121 ccgagcacca ccgtgatgtc cggatccttg gcgggcgcgg tgatgaccac cttcttggcg
      181 ccggcctgca ggtgcttgga cgaggtggcg cggtcgcgga agatcccggt ggactccacc
      241 acgatcccca cacccatctc gccccagggc agcgtggccg ggtccttggc cgagcagacc
      301 cggatctccc gcccgttgac gaagatcgcc tcgcccttcg ccttcacctc ggcgtcgagc
      361 gtgccgtgga cggagtcgtg cttcaggaga tgggccaggg tcttggcgtc ggccaggtcg
      421 ttgaccgcca ccacctcgat gtccgacgac tccagggccg cgcgaaagaa cacccggccg
      481 atccgtccga acccgttcac tccgacgcgc actgccatgg gcgctcccct tcctgtttgt
      541 ctcggtttgt cgaatacttc cggtccacgg aggcttccga ttatagcgag cccggggcat
      601 gaggcgcaag accgggtccg acggcccgga tcgggtccgg gcgggccctc cggaggcgcc
      661 ctagacgacg cgggcgaccg cgaggacgcc gacgatgcgg ggcgcggcgt cctcgaggca
      721 ccgggcgcac tcggagagcg tggcgccggt ggtcaggata tcgtccacca ggaccacgtg
      781 gcgacccgcg accagctcgg gccggcgcag ccggaacgcg cgcctcacgt tcgcgcgccg
      841 ggcggctccg tcgagcgagg tctgggacgg cgtcgccacc gcgcgcacca ggacgtcgtc
      901 gcgcaccggg cagccccacg cccggccgag ccgccgcgcg agcagccgcg actgattgaa
      961 gccgcgctcg cgctcccgtc gcgggtgcag cgggaccggc accagcagat cgggggcccc
     1021 gccgggcagt cgcccctcga ccgcctcgac cagcgcgtcg ccgagggggc cggcgagggc
     1081 gcgatggccg ccgaacttga aggcgtgtag ggcctcgcgg gccacgtcct cgtagcggag
     1141 cgccgcgcgc gcatacgcga aggcgggcgg ccgtcgtcgg caggccgcgc agagggcgcc
     1201 gccctccgcg cgcgtcggag cagccgtcgt cggcccggcc gccaccggga agcccgccat
     1261 cggctgaccg cagacgcggc accacggagg cccgatgcgc ggaagcccgg cccagcaggc
     1321 cccgcagagc ggatcgcgcc ggccctcctc gaggcgcgcc cggcacaccg ggcagaacgg
     1381 cgggaagacg aggtccagca gggcacccgc ccaccgcggc cagagcgcgg ggtccgggcg
     1441 aggacggtct agggtcggga cgtgttccag cgacggcacg acggacaacg atctacccag
     1501 cgcgggtgga gggctccgca cgtcgcgcag tgatgcggcc actggacgct gtcgctcagg
     1561 cgaagcgcgc gccggtactc ttccatcgcg tcgcggaact ggccgcgacg ctcgaggatc
     1621 gcgcccaggt aggcgtggat gagcgggagg tcggccgccc gcacctccat cttctggaac
     1681 tgctccgcgg cctcgtccag catcgccagc tcgaagtaga cgcggccgag cccgaaggcc
     1741 agcgcgaggt tgtcgggatg ccggtcgagc gcgttccggt agagggagat catgcgggtg
     1801 ggccggccct cctcgcggtg ccgctgctcg atacgggcca gcaccggcag agcgggctgc
     1861 ggcgcctccg cggcccgctc ccacacccgg agcgcgtcgc gcggctcgcc cgccttcaga
     1921 tgggcctcgc cgagcaggag ggccgcgggc acgaagtccg gctgcatgcg gagggcgtcc
     1981 ttgaagtgcg acaccgcgct cgcgaggtcg ccccgctcga ggcgctcccg cccgacctcg
     2041 tagtgcagcc cggccagggt cacctcctcg gcggcccgct cctcgcgcgg caccccggcc
     2101 aggatctggg tctgggccac cagagcgtcg gcgaagcgcc cgcgctcgat cagggcgtcg
     2161 cgcgcggcgc gggccggcgc gggcgccggc tcggcggcgc ggagcggcgc tgtgtccggg
     2221 cggagcgagg cccgcggcgc cgcgaccgcc accggcccgc gggcgtatgg gcgcgtcagg
     2281 tcccgcagcc atccgagcac cagcgcgagc aacgcgcccg ccccgaagac ccccaccacc
     2341 agacccatga gcggcacgtc gaccgcccag tcgccgccaa gggccaggtg cgcgcggccg
     2401 ggattgagcg aggcgagata ggtgaccagg atggcgaaga gcagaccgac gacgaggccg
//
LOCUS       DATMCV010000656         2712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_902944, whole genome shotgun sequence.
ACCESSION   DATMCV010000656 DATMCV010000000
VERSION     DATMCV010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902944"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..855)
                     /locus_tag="VNU03_15045"
     CDS             complement(43..855)
                     /locus_tag="VNU03_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75836.1"
                     /translation="MRPARDRCWVAWGRAGERRPEPRYFRTRFEAEAFILGLLVGESG
                     AALVGFDFAFGYPADAGLPSGRALCARLDGLIRDAADGTNNRFEVAGILNREIQAALG
                     GGFDGPFWGHPSGREYPHLAPTRPRPFPRGISDGRLVERRLAARRIQSPWKLFTRASV
                     GSQTLVGLPAVHRLLTDPALEARARLWPFETAWDAAVGADSVVIAELWPSLIDGRDQP
                     YPIKDACQVAAVRDWALDSPDALGRSLARPAGLTDDEDRTAREIEGWIAGNV"
     gene            complement(909..1937)
                     /locus_tag="VNU03_15050"
     CDS             complement(909..1937)
                     /locus_tag="VNU03_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP75837.1"
                     /translation="MKALAFHEHGGLDKLKYQDVPDPKIGAGDVLVRVRAAALNHLDL
                     FVREGLPGLTLPMPFWTGCDIAGDVAQVGAEVRDVAVGDRVAVNPNLYDGRCEYCLRG
                     EHSLCVRYGILGEHTHGGLAEYVKVEGRHVVKLPDAIRYEDAAAFVLVNMTAWRMLVT
                     QARLRAGEDLLILGVGGGVSSTAVQIGKLCGARVWVTSSSDEKLERARALGADECINY
                     AKDDWVKVIGQKTGRRGVDVILENVGAATWKGALRTVAKGGRIVTCGATSGPIAETDI
                     RLVFWKQLSIIGSTMSNDAEFSTVMGQLFAGRLRAIVDTVMPLADGVEAQRRLAEGKQ
                     FGKIVLTP"
     gene            complement(1951..>2712)
                     /locus_tag="VNU03_15055"
     CDS             complement(1951..>2712)
                     /locus_tag="VNU03_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP75838.1"
                     /translation="AQGHQCIAKPKRLTWEAAGCFLLCASTAYRMLMGWEPHTVEKGD
                     AVLVWGATGGLGSLALEIVRAQGGRAVAVVSDDAKRKFCMDHGAAGVINRSQFDHWGV
                     MPDTKDGKAYGNWAKGARGFGKAIWDALGERVSPRIVFEHPGEATLPTSEFVCATGGM
                     IVICAGTTGYNVTLDLRYHWMRQKRFQGSHLSNDQQALAVTKLVDAGKVDPCLSQTYR
                     FDELPECHQLMLDNKHPYGNMAVLVNAPKTGLGAS"
BASE COUNT          387 a          999 c          931 g          395 t
ORIGIN      
        1 cccccgccac gatcagcacc agattggggt caggtcttac attcatacat ttccggcgat
       61 ccagccctcg atctcgcggg cggtgcggtc ctcgtcgtcg gtgaggccgg ccgggcgggc
      121 gagtgatcgt ccgagggcgt cgggcgagtc cagcgcccag tcacgcaccg ccgcgacctg
      181 gcacgcatcc ttgatcggat agggctggtc ccggccgtcg atcaggctcg gccagagctc
      241 ggcgatgacg acgctgtccg cgccgaccgc cgcgtcccac gcggtctcga agggccagag
      301 ccgcgcgcgc gcctcgagcg ccgggtccgt gagcaggcga tggaccgcgg gcaggcccac
      361 cagggtctgc gagccgaccg aggcgcgcgt gaagagcttc cacgggctct ggatgcgccg
      421 cgcggccagc cgccgctcga ccagccggcc gtcgctgatg ccccggggga agggccgcgg
      481 ccgcgtcggg gcgaggtgcg ggtactcgcg gcccgaggga tggccccaga acgggccgtc
      541 gaagcctccg ccgagcgcgg cctggatctc gcggttcagg atccccgcga cctcgaaccg
      601 attgttcgtg ccgtcggccg cgtcgcggat caggccatcc agccgggcgc agagggcgcg
      661 cccggaggga agccccgcgt cggccggata gccgaacgcg aagtcgaagc ccacgagggc
      721 cgccccggac tcgcccacga gcaggccgag gatgaaggcc tccgcctcga accgcgtgcg
      781 gaagtagcgt ggctccggcc gtcgctcccc ggcccgtccc caggcgaccc agcagcggtc
      841 tcgcgcgggc ctcaccgggc ctcgtgtcga agccgccgac cagtcggcga cgacgatcag
      901 ccggaagctc agggcgtgag gacgatcttg ccgaactgct tgccctccgc gaggcgccgc
      961 tgcgcctcga caccgtccgc gagcggcatc acggtgtcca cgatggcccg cagccgtccc
     1021 gcgaagagct gccccatgac cgtgctgaac tcggcgtcgt tcgacatcgt ggagccgatg
     1081 atcgagagct gcttccagaa cacgagccgg atgtcggtct ccgcgatggg gcccgacgtc
     1141 gcgccgcagg tgacgatgcg cccacccttg gccacggtgc ggagcgcgcc cttccaggtg
     1201 gcggcgccca cgttctccag gatcacgtcc accccgcgcc gcccggtttt ctgcccgata
     1261 accttcaccc agtcgtcctt cgcgtagttg atgcactcgt ccgcgcccag ggcccgcgcg
     1321 cgctcgagct tctcgtcgct cgacgacgtg acccacaccc gcgcgccgca gagcttgccg
     1381 atctgcaccg cggtggagga gacgccgccg ccgaccccca ggatcaatag atcttcgccc
     1441 gcgcgcagcc gggcctgggt gaccagcatg cgccaggcgg tcatgttcac gaggacgaag
     1501 gccgctgcgt cctcgtagcg gatcgcgtcc ggcagcttca ccacgtgccg gccctcgacc
     1561 ttgacgtact cggcgaggcc tccgtgagtg tgctcgccca ggatgccgta gcgcacgcac
     1621 aggctgtgct cgccgcggag gcagtactcg caccgcccgt cgtagagatt ggggttcacc
     1681 gcgacccggt cgcccaccgc gacgtcgcgg acctcggccc ccacctgggc cacgtcgccc
     1741 gcgatgtcgc agccggtcca gaacggcatc ggcagggtga gaccgggcag gccctcgcgg
     1801 acgaagagat cgaggtgatt cagcgccgcg gcgcggacgc gcaccagcac gtcgccggcg
     1861 ccgatcttcg ggtcggggac gtcctggtac ttgagcttgt cgagcccgcc gtgctcgtgg
     1921 aacgcgagcg ccttcacgcg cgcggccgga tcaggacgct ccgagccccg tcttgggcgc
     1981 gttcaccagc accgccatgt tcccgtacgg gtgcttgttg tccagcatga gctggtggca
     2041 ctcgggcagc tcgtcgaagc ggtaggtttg cgagagacac ggatcgacct tgcccgcgtc
     2101 gaccagcttc gtcaccgcca gcgcctgctg gtcgttcgag aggtggctgc cctggaagcg
     2161 cttctgccgc atccagtggt agcgcaggtc gagcgtgacg ttgtagccgg tggtgcccgc
     2221 gcagatcacg atcatgcccc cggtggcgca cacgaactcc gaggtgggca gcgtcgcctc
     2281 gcccgggtgc tcaaagacga tgcgagggct gacgcgctct cccagcgcgt cccagatggc
     2341 cttgccaaag ccccgcgcgc ccttggccca gttgccgtag gccttgccgt ccttcgtgtc
     2401 cggcatcacc ccccagtggt cgaactggga gcggttgatg accccggccg cgccgtgatc
     2461 catacagaac ttcctcttgg cgtcgtcgga gaccaccgcg accgcgcggc ccccctgcgc
     2521 gcgcacgatc tcgagcgcga ggctgccgag cccgccggtg gcgccccaca ccagcaccgc
     2581 gtcgcccttc tccacggtgt gcggctccca gcccatcagc atgcggtacg cggtggacgc
     2641 gcagaggagg aagcagccgg ccgcctccca ggtgaggcgt ttcggcttgg cgatgcactg
     2701 gtgcccctgg gc
//
LOCUS       DATMCV010000657         4803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_369738, whole genome shotgun sequence.
ACCESSION   DATMCV010000657 DATMCV010000000
VERSION     DATMCV010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369738"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1049
                     /locus_tag="VNU03_15060"
     CDS             54..1049
                     /locus_tag="VNU03_15060"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWP75839.1"
                     /translation="MILRIALVVAVVLGLAGPAPAQVVTLKLATIVGNDNPFTTSAYK
                     FKEIAEAKSGGRIKVEVYPAGQLTKNEIVAIEGMQIGTVDIAAVSLPSAGGKFDPRLL
                     APDVPFLYQSREHVWRVMDGPVGQEMLKYFDPLGAKAFCYGGGFGFRGVLSNKRPVFV
                     PDDLKGQTIRVPPIPTLVEVFKQFGANPVPMIWGEVYMAVRQGTVDGLELPVTTAVSD
                     KFHEISKYYSKTLHSYPAAAWMMAKAKYDGLPADLKKVIDETMTASCAQHRQDELKAE
                     VEGLAIMKAKGVQINDVKDLRQFQERAQPVWKFLEQKAGKELVDRVIAEAKAAAK"
     gene            1063..1500
                     /locus_tag="VNU03_15065"
     CDS             1063..1500
                     /locus_tag="VNU03_15065"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HWP75840.1"
                     /translation="MIIDWICLGLMAALVVDVFLGVFSRYVLHATFQWYDEVARLCFV
                     WMVFLGAAVAVRRGAHFRLHLLIDRFGPRLRRATDLGVALVVLLFGVVLVAGGVAMYP
                     VARRQVSDSLELSMLWFHGALPVGGALMILFSLPHLWRLIRAR"
     gene            1511..2788
                     /locus_tag="VNU03_15070"
     CDS             1511..2788
                     /locus_tag="VNU03_15070"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HWP75841.1"
                     /translation="MILVCLVFLALLAVGVPVAVTVGLAGYVGALIAAPAPLAVTVQT
                     ILHQVDSFVLLSLPLFILAGALMETGGIAHRLVRLAVALVGWVRGGLGMAVVAAEYIF
                     SGISGSTVADVSAVAATTIPGMVRAGYSRELAVAVVSAASAMGILVPPCILMIVIGSV
                     ASLSVAALFTAGFLPAVVMAIVLMGYIWRTAGRMGIAAEPKPTARELWVAFRQALIPL
                     GMPAIIFGGILGGIMTPTEASVLAVLYALIVGLFVYREIKWTDLPAIMMNSAMITGAV
                     GLLLGTAGVVSWFLTVQQMPAAMIRLMTVVPGSSLVFLFITAAIFIFFGAVIEGLPAV
                     VILLPSLLPVAGGLGIDPIHYAIVIVAAVGIGLFLPPIGVGLFIACGIAELSVDRATR
                     AMLPFVLVLVIGLVIIILVPEITLILPRLFRLL"
     gene            complement(2792..3481)
                     /locus_tag="VNU03_15075"
     CDS             complement(2792..3481)
                     /locus_tag="VNU03_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75842.1"
                     /translation="MLDVEAIHTYYGDSHVLHGVSLRVAPGEAVALLGRNGAGKTTAI
                     RSIVGFTPPRAGRVVFEGRAIERWPSYRIARSGLALVPQGRRIFAPLSVRENLLLGAR
                     PEGWTLERVFALFPRLREREQQSGGTLSGGEQQMLAIGRALLTNSRLILLDEPSEGLA
                     PLIVREIGRILLALKAERLSLLLVEQNYHLALRVADRVYVMNKGQIVYEGTPSGLEAD
                     EEVKRRYLGVA"
     gene            complement(3484..4230)
                     /locus_tag="VNU03_15080"
     CDS             complement(3484..4230)
                     /locus_tag="VNU03_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75843.1"
                     /translation="MSVPLLAVEALTRAFGALSALNRVSFQVAPRERRAIIGPNGAGK
                     TTLFNVITGQLAPTGGGIRLEGTPIDGLSPHAVARRGISRSFQRNNLFPKLAVGENLR
                     LAAAARAPGTWDLLGSLARRRGPIDRARAVAEIVGLADRLGAPAGLLSYGEQRQLEVG
                     VALATGPRLLLLDEPTAGMSPEETQRMTRMLEALPRDVTLLIIEHDMDVVGSLADRVT
                     VLHYGEVLTEGTFAEVKADPRVYEVYLGQA"
     gene            complement(4227..>4803)
                     /locus_tag="VNU03_15085"
     CDS             complement(4227..>4803)
                     /locus_tag="VNU03_15085"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75844.1"
                     /translation="QMFHAYTFQVSWLGAEDGLVGIPRPTVPGVDLASLRGFHAYVLT
                     LVVLVALALWRIVRSPFGAVLRGIHENEARMEALGYAVDRYKLLAFVIAGIVAGVAGS
                     LYTQFNGSITPDAFYWKTSGEALLMVIIGGTGTLGGALLGAAAFILLQSLVSTYTERW
                     MLILGTTFILFVLFAPGGIVGALRGRIGLRA"
BASE COUNT          656 a         1687 c         1699 g          761 t
ORIGIN      
        1 agcgtcccag acaaagcggt catccgtcgt cgacgccgcc ggaggcactc gtcatgattc
       61 tgcgcatcgc tctcgtcgtg gccgtcgtgc tcgggctcgc ggggcccgca cccgcccagg
      121 tcgtcaccct caagctggcc accatcgtcg gcaacgacaa cccgttcacg acgtcggcgt
      181 acaagttcaa ggagatcgcg gaggccaagt ccggcggccg catcaaggtg gaggtctacc
      241 ccgcgggcca gctcaccaag aacgagatcg tggcgatcga gggcatgcag atcggcacgg
      301 tggacatcgc cgcggtgtcc ctgccgtcgg cgggcggcaa gttcgatccg cgcctcctcg
      361 cccccgacgt gccgttcctc taccagagcc gcgagcacgt gtggcgggtg atggacggac
      421 cggtcgggca ggagatgctg aagtacttcg atcccctcgg cgcgaaggcc ttctgctacg
      481 ggggcggctt cggcttccgc ggcgtgctct ccaacaagcg cccggtgttc gtgcccgacg
      541 acctgaaggg gcagaccatc cgcgtcccgc ccatccccac cctggtcgag gtcttcaagc
      601 agttcggggc caacccggtg ccgatgatct ggggcgaggt gtacatggcg gtgcgccagg
      661 gcacggtgga cgggctggag ctaccggtca ccaccgcggt ctccgacaag ttccacgaga
      721 tcagcaagta ctactcgaag acgctccact cctacccggc cgccgcgtgg atgatggcca
      781 aggccaagta cgacggcctg cccgccgatc tcaagaaggt gatcgacgag accatgaccg
      841 cctcctgcgc ccagcaccga caggacgagc tcaaggccga ggtcgagggc ctcgcgatca
      901 tgaaggccaa gggcgtgcag atcaacgacg tgaaggacct gcgccagttc caggagcgcg
      961 cccagccggt gtggaagttc ctcgagcaga aggcgggcaa ggagctggtc gaccgggtga
     1021 tcgccgaggc gaaggcggcc gcgaagtagc cggccctacc aggtgatcat cgactggatc
     1081 tgcctggggt tgatggcggc cctggtggtg gacgtcttcc tgggggtctt cagccgctac
     1141 gtgctgcacg cgacgttcca gtggtacgac gaggtcgcgc gcctctgctt cgtgtggatg
     1201 gtcttcctcg gcgccgcggt ggcggtccgg cgtggcgcgc acttccgcct gcatctcctg
     1261 atcgaccgct tcggcccccg cctgcgccgc gcgaccgatc tcggggtggc cctggtggtg
     1321 ctgctcttcg gcgtggtgct ggtggcgggc ggcgtcgcaa tgtacccggt ggcgcggcga
     1381 caggtgagcg actcgctcga gctgtcgatg ctgtggttcc acggcgccct gccggtcggc
     1441 ggcgccctga tgatcctgtt ctccctgccc cacctctggc gtctcatccg ggcccgatga
     1501 gcggggcccg atgatcctcg tctgcctcgt cttcctggcc ctgctcgcgg tgggcgtgcc
     1561 ggtcgcggtc acggtcggcc tcgccggcta cgtgggcgcg ctgatcgcgg cgcccgcgcc
     1621 gctggcggtg acggtgcaga ccatcctcca ccaggtggac tccttcgtgc tgctctcgct
     1681 gccgctgttc atcctggccg gcgcgctgat ggagaccggc ggcatcgcgc atcgcctggt
     1741 gcgcctggcc gtcgcgctgg tgggctgggt gcggggcggg ctcggcatgg cggtggtggc
     1801 cgcggagtac atcttctccg gcatctccgg ctccacggtg gcggacgtct ccgcggtggc
     1861 cgcgaccacc atcccgggca tggtacgcgc gggctacagc cgagagctgg cggtggcggt
     1921 ggtgtcggcc gcgtccgcga tgggcatcct ggtgccgccc tgcattctca tgatcgtgat
     1981 cggctcggtg gcgagcctct cggtggccgc gctcttcacc gccggcttcc tgcccgcggt
     2041 ggtgatggcg atcgtgctga tgggctacat ctggcgcacc gccgggcgca tgggtatcgc
     2101 ggccgagccc aagccgaccg cacgcgagct gtgggtggcc ttccggcagg ccctgatccc
     2161 gctcggcatg cccgcgatca tcttcggcgg catcctgggc ggcatcatga cccccaccga
     2221 ggcctcggtg ctggcggtgc tgtacgccct gatcgtcggc ctcttcgtct accgcgagat
     2281 caagtggacg gacctgcccg cgatcatgat gaactcggcg atgatcaccg gcgcggtggg
     2341 gctgctcctg gggaccgcgg gcgtggtctc gtggtttctc accgtgcagc agatgccggc
     2401 cgcgatgatc cgcctcatga cggtggtgcc gggcagcagc ctggtcttcc tcttcatcac
     2461 cgccgcgatc ttcatcttct tcggcgcggt catcgagggg ctgcccgcgg tggtgatcct
     2521 gctgccgagc ctcctgccgg tggcgggcgg gctcggcatc gatccgatcc actacgcgat
     2581 cgtgatcgtg gccgcggtgg gcatcggcct cttcctgccg ccgatcgggg tgggcctctt
     2641 catcgcgtgc gggatcgccg agctgtcggt ggaccgggcc acccgcgcga tgctgccgtt
     2701 cgtgctggtg ctggtgatcg ggctcgtgat catcatcctg gtgcccgaga tcaccctcat
     2761 cctcccccgc ctcttccgcc tcctctgagg gtcacgcgac gccgagatac cgccgcttca
     2821 cttcctcgtc cgcctcgagc cccgacggcg tcccctcgta gacgatctgc cccttgttca
     2881 tcacgtagac ccggtcggcc acccgcagcg ccaggtggta gttctgctcc acgagcaaga
     2941 gcgacagccg ctccgccttg agcgcgagca ggatgcggcc gatctcgcgc acgatcagcg
     3001 gagccaggcc ttccgacggc tcgtcgagca ggatcaggcg gctgttggtc agcagggcgc
     3061 gaccgatggc gagcatctgc tgctcgccgc cggagagggt gccgcccgac tgctgctcgc
     3121 gctcgcgcag gcgcggaaag agcgcgaaca cccgctcgag cgtccagccc tcgggccggg
     3181 cgcccagcag caggttctcg cgcaccgaca gcggcgcgaa gatgcggcgg ccctgcggca
     3241 cgagggcgag cccgctccgc gcgatgcggt agctgggcca gcgctcgatc gcgcggccct
     3301 cgaagaccac gcgccccgcg cgcggcgggg tgaagcccac gatgctgcgg atcgcggtgg
     3361 tcttgcccgc cccgttgcgc ccgagcagcg ccaccgcctc tccgggggcc acgcggaggg
     3421 agacgccgtg cagcacgtgg ctgtcgccgt agtaggtgtg gatggcctcg acgtcgagca
     3481 tgctcacgcc tgccccaggt agacctcgta gacgcgcgga tcggccttca cctcggcgaa
     3541 ggtgccctcg gtgagcacct cgccgtagtg gagcacggtg acccggtcgg cgagcgagcc
     3601 caccacgtcc atgtcgtgct cgatgatgag cagggtcacg tcgcgcggca gcgcctccag
     3661 catgcgggtc atgcgctggg tctcctcggg cgacatgccg gcggtgggct cgtccaggag
     3721 caggagccgc ggcccagtgg ccagcgcgac gcccacctcg agctggcgct gctcgccgta
     3781 ggagaggagg ccggcgggcg cgccgagccg atccgcgagg cccacgatct ccgcgaccgc
     3841 ccgcgcccgg tcgatcggcc cgcgccggcg cgcgaggctc cccagcagat cccacgtccc
     3901 gggcgcgcgg gccgcggcgg ccagccgcag gttctctccc accgcgagct tcgggaacag
     3961 gttgttgcgc tggaatgagc gggagatgcc ccggcgggcc accgcgtgcg ggctgaggcc
     4021 gtcgatcggc gtgccctcga gccggatccc gccgccggtg ggcgccagct gtccggtgat
     4081 cacgttgaag agcgtggtct tgcccgcgcc gttggggccg atgatggcgc ggcgctcgcg
     4141 cggcgccacc tggaaggaca cgcggttcag cgcgctcagc gctccgaacg cgcgggtcag
     4201 cgcctcgacg gccaggaggg ggacgctcac gcgcgcagcc cgatgcgccc gcgcagcgcg
     4261 cccacgatcc cgccgggggc gaagagcacg aacagaatga aggtggtgcc gaggatcagc
     4321 atccaccgct cggtgtaggt gctgaccagg ctctggagca ggatgaacgc ggccgcgccc
     4381 agcagcgccc cgcccagggt cccggtgccg ccgatgatca ccatcagcag cgcctcgccc
     4441 gacgtcttcc agtagaacgc gtccggggtg atcgagccgt tgaactgggt gtagagcgag
     4501 ccggccactc ccgccacgat ccccgcgatc acgaacgcca gcagcttgta gcggtccacc
     4561 gcgtagccca gcgcctccat gcgcgcttcg ttttcgtgga tgccgcgcag cacggcgccg
     4621 aacggcgagc ggacgatgcg ccagagcgcg agcgccacca gcacgaccag cgtgagcacg
     4681 taggcgtgga agccgcgcag gctcgcgagg tccacgcccg gcaccgtggg gcgcgggatc
     4741 ccgacgaggc cgtcctcggc cccgagccag gagacctgga aggtgtaggc gtggaacatc
     4801 tgc
//
LOCUS       DATMCV010000658         5377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_233905, whole genome shotgun sequence.
ACCESSION   DATMCV010000658 DATMCV010000000
VERSION     DATMCV010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233905"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VNU03_15090"
     CDS             <1..831
                     /locus_tag="VNU03_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HWP75845.1"
                     /translation="ASEVRHRAIFGLLGRTRPLAVHEAAFVRTLTDRPVKVALPGPYL
                     LSRIMWMECISDRAYRSREHLAEDIVRVLREELHFLLAAGVALVQLDEPVLSEVVFTG
                     AKNARSFMCGALSEKGDAATELAFAGTLLDQVVAGLPRERLGLHVCRGNWTRDEKAAL
                     AGDYRPLLPLLERVSVGTLFLELCTERAGEIEVLKALPSTTRVGVGVVNQKHERVESA
                     DEIVARGRRAIACFGAERVLLTPDCGFATFADNPVASAQVAEAKLLAIRRAAEILRRG
                     "
     gene            complement(818..2416)
                     /locus_tag="VNU03_15095"
     CDS             complement(818..2416)
                     /locus_tag="VNU03_15095"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HWP75846.1"
                     /translation="MAWSLLIRNGTVVDGSGAPARAADVAVEGDRIAAVGPGLAGEAA
                     RVIDAQGLMVAPGFIDAHSHSDLVYDKCPSAESKVRQGVTTEVVGMCSFSPAPVAPGR
                     ADLVQEWAGGIGGKIPITWNTFGEYLDHLRGLGPTVNIVQFVGHGALRLAAVGPAARP
                     VTPEEQKGMERLLAEALDAGAFGFSTGLVYAPSVYGNTDELVGLARSMASRRGLYFSH
                     IRGEAATLEQAAEEAITIGERGGVPVQIAHVKASGRENWGKMDRVLRMIDSARARGVD
                     VTGDVYPYPAGSTKMDNLMPAWMHDGGISKLLERLTDPKARQRAIADCLVDGERWRTG
                     SGGMGWDEIMIATCSKPELAGIHIAELARRTGKEPAQAMMDLVLEERAGVSMVVFSQS
                     EDNVGTALSYAHTMVGSDSLSLHAGPGPHPGKPHPRSYGTFPRVLGVYCREKRLFSWE
                     TAVHKMTGMAATKIGLKDRGILQAGRAADVTVFDPATVSDAATFPDPHQYPVGIPYVI
                     VNGRVVVDGAQYHALPAGRVLTPA"
     gene            complement(2422..3021)
                     /locus_tag="VNU03_15100"
     CDS             complement(2422..3021)
                     /locus_tag="VNU03_15100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWP75847.1"
                     /translation="MKARMATIALVALLAALLLPVAGSIAPAGAQQPTQPAPPAPTAP
                     KEAPKGGKKVKQTAVITMEKGGEINIEFFPEDAPKTVENFVTLAKKGFYDGVTFHRVE
                     PNFVVQGGDPKGNGTGGPGYTIKDEFNKQKHVRGVVAMARTQAPNSAGSQFYITLAPA
                     HFLDNQYTVFGRVTSGMEIVDKIKVGDKMKSVKIIEAAQ"
     gene            3119..4930
                     /gene="typA"
                     /locus_tag="VNU03_15105"
     CDS             3119..4930
                     /gene="typA"
                     /locus_tag="VNU03_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWP75848.1"
                     /translation="MPQGPNVRPDIRNVAIIAHVDHGKTTLVDAMLWQSGIFRANEHV
                     AERVMDSIDLEREKGITIMAKNTAIHYQGVKINIVDTPGHADFGGEVERTLTLVDGVL
                     LLVDAAEGPLPQTRFVLKKALEAGLTPVVVINKIDRQDARAAEVLDEVYDLFIDLDAA
                     VEQLEFPVLYTDARKGIAKRSLTDESKTLAPLFETLLETIPAPKFEEGHGLQLRAASL
                     DWDDYVGRLIIGRIVNGRVRQYDRVAVVHRDGTIENAKITVLYGYEGLKRVEVPEMSA
                     GDIVAVAGIEAMEIGETIADPEKPIALPVMHIDEPTVAMLFSSNVSPFAGKEGKFVTS
                     TQLRERLWREKRSNVALQVDETDSPDTFKVSGRGELQLAILIEMMRREGYEMEVSKPQ
                     IITHEENGKTLEPMEHLVVDVPDDFIGAVTQKLGPRKGQMVKMVNHGTGRVRLEYRIP
                     SRGLIGYRSEFLTDTRGTGLLNHLFDGYADWQGDIPHRASGAMVADRTGKTTAYAIDH
                     LQPRGILFLGPGEPVYEGQVVGENARDNDLDVNITKEKKLTNVRSSTSDEAVRLTPPH
                     LMNLEQSIEWIREDELLEITPKSLRLRKKSLSGRRRF"
     gene            complement(4992..>5377)
                     /locus_tag="VNU03_15110"
     CDS             complement(4992..>5377)
                     /locus_tag="VNU03_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP75849.1"
                     /translation="IEFYQRAFNAKELLRIPAPNERIGHAEIKVGDSVLMMADEHPEM
                     DAYAPKHFGGSPVSLLLYVTDVDAMFRQALAAGATEVRPVSDQFYGDRSGTLKDPFGH
                     TWHLSTHKEDVSMEEMARRMAAMKQ"
BASE COUNT          819 a         1875 c         1848 g          835 t
ORIGIN      
        1 gcctccgagg tgcgccaccg cgcgatcttc ggccttctcg gccgcacgcg gcccctcgcg
       61 gtccacgagg ccgcgttcgt gcggacgctg accgatcggc cggtgaaggt ggcgctgccc
      121 ggcccctatc tgctctcgcg catcatgtgg atggagtgca tctccgaccg ggcctaccgc
      181 tcgcgcgagc acctggcgga ggacatcgtc cgcgtgctcc gcgaggagct gcacttcctg
      241 ctcgcggcgg gcgtggccct ggtgcagctc gacgagccgg tgctctccga ggtggtcttc
      301 accggggcca agaacgcgcg gagcttcatg tgcggcgcgc tcagcgagaa gggtgacgcg
      361 gcgaccgagc tcgccttcgc ggggaccctg ctcgatcagg tcgtggcggg cctgccgcgc
      421 gagcgcctgg gcctccacgt ctgccggggc aactggacgc gcgacgagaa ggccgcgctg
      481 gccggcgact accggccgct cctgcccctg ctcgagcgcg tgagcgtcgg cacgctcttc
      541 ctcgagctct gcaccgagcg ggcgggcgag atcgaggtgc tgaaggcgct gccatccacg
      601 acccgcgtcg gggtcggagt ggtcaaccag aagcacgagc gggtggagag tgcggacgag
      661 atcgtggcac gcggacgccg cgccatcgcg tgcttcggcg ccgagcgcgt cctgctgacc
      721 cccgactgcg ggttcgccac cttcgccgac aacccggtcg cgtcggctca ggtggcggag
      781 gccaagctcc tcgcgatccg ccgggccgcg gagatcctca ggcgggggtg agcacccggc
      841 cggccggcag cgcgtggtac tgcgcgccgt ccaccacgac ccgaccgttg acgatgacgt
      901 acgggatccc caccgggtac tggtgcggat ccgggaaggt ggccgcgtcg ctcacggtgg
      961 ccggatcgaa gaccgtcacg tccgccgcgc ggccggcctg caggattccc cggtccttga
     1021 gcccgatctt ggtcgcggcc atcccggtca tcttgtgcac cgcggtctcc cacgagaaga
     1081 ggcgcttctc gcggcagtaa accccgagta cccgggggaa cgtcccgtag ctgcgggggt
     1141 ggggcttccc gggatggggc cctggaccgg cgtggagcga gagcgagtcc gagcccacca
     1201 tggtgtgagc ataggagagc gccgtcccca cgttgtcctc gctctgggag aagaccacca
     1261 tcgagacgcc ggcgcgctcc tccagcacca ggtccatcat ggcctgcgcc ggctccttgc
     1321 cggtgcggcg ggccagctcc gctatgtgga tgccggccag ctcgggcttc gagcaggtgg
     1381 cgatcatgat ctcgtcccag cccatgccgc ccgagccggt gcgccagcgc tcgccgtcca
     1441 ccaggcagtc ggcgatcgcg cgctgccgcg ccttcgggtc ggtcaggcgc tcgagcagct
     1501 tcgagatgcc gccgtcgtgc atccacgcgg gcatcaggtt gtccatcttg gtgctgcccg
     1561 ccgggtaggg atagacgtcg ccggtcacgt cgaccccgcg ggcgcgcgcg ctgtcgatca
     1621 tgcggaggac gcggtccatc ttgccccagt tctcgcggcc cgaggccttg acgtgggcga
     1681 tctgcaccgg caccccaccc cgctcgccga tggtgatcgc ctcctccgcg gcctgctcga
     1741 gggtggccgc ctcgccgcgg atgtgggaga agtagagccc gcgccggctc gccatgctgc
     1801 gcgcgagccc caccagctcg tcggtgttgc cgtagacgct gggggcgtag acgagcccgg
     1861 tggagaagcc gaaggccccc gcatcgagcg cctccgcgag caggcgctcc atgcccttct
     1921 gctcctcggg ggtaaccggg cgcgccgcgg ggccgaccgc ggccagtcgc agcgcgccgt
     1981 gccccacgaa ctgcacgatg ttgacggtgg ggccgagccc gcgcaggtga tcgagatact
     2041 cgccgaaggt gttccaggtg atcgggatct tgccgccgat gccacccgcc cactcctgca
     2101 ccaggtccgc gcggccggga gcgaccggcg cgggcgagaa gctacacatg cccaccacct
     2161 cggtggtcac gccttgccgc accttcgact cggccgacgg gcacttgtcg tagaccaggt
     2221 ccgagtgcga gtgcgcgtcg atgaagcccg gggccaccat caggccctgg gcgtcgatca
     2281 cccgggccgc ctcgccggcc aggcccgggc ccaccgcggc gatgcggtcg ccctcgaccg
     2341 ccacgtcggc tgcacgcgcg ggagcgccgc tgccgtccac caccgtgccg ttccggatga
     2401 ggagcgacca ggccatggcg cctactgggc ggcctcgatg atcttcaccg acttcatctt
     2461 gtcgcccacc ttgatcttgt ccacgatctc catgcccgag gtgacccggc cgaagacggt
     2521 gtactggttg tcgaggaagt gggcgggggc cagcgtgatg tagaactggc tgcccgccga
     2581 gttgggggcc tgggtgcggg ccatcgccac caccccgcgg acgtgcttct gcttgttgaa
     2641 ctcgtccttg atggtgtagc ccgggccgcc ggtcccgttg cccttgggat cgccgccctg
     2701 cacgacgaag ttgggctcga cccggtggaa ggtgaccccg tcgtagaaac ccttcttggc
     2761 cagcgtcacg aagttctcca ccgtcttggg cgcgtcctcc gggaagaact cgatgttgat
     2821 ctcgccgccc ttctccatgg tgatgaccgc ggtctgtttc accttcttcc ctcccttggg
     2881 cgcttctttg ggtgctgtgg gtgccggggg tgccggttgc gtgggctgct gcgcgccggc
     2941 cggcgcgatc gagccggcga cgggcagcag gagcgcggcg agcagcgcga cgagcgcgat
     3001 ggtggccatg cgagccttca ccgatgagtc ctccttgacg tgggaaaccg gggtggacgc
     3061 gtcattatgc cccaccgtcg cgccggtccg ccactctatc cgtggtacag tgaaaggcat
     3121 gccgcagggc cccaacgtcc gacccgacat cagaaacgtc gccatcatcg cccacgtcga
     3181 ccacgggaag actacgctcg tggacgccat gctctggcag tccggcatct tccgggccaa
     3241 cgagcacgtg gccgagcggg tgatggactc gatcgacctg gagcgcgaga agggcatcac
     3301 gatcatggcc aagaacacgg ccatccacta ccagggcgtg aagatcaaca tcgtggacac
     3361 gcccggccac gccgacttcg ggggcgaggt ggagcggacg ctgaccctgg tcgacggggt
     3421 gctgctgctg gtcgacgcgg cggagggccc cctgccccag acccgcttcg tgctgaagaa
     3481 ggcgctcgag gccgggctca ccccggtggt ggtcatcaac aagatcgacc gccaggacgc
     3541 ccgcgcggcc gaggtcctgg acgaggtcta cgacctcttc atcgacctgg acgcggcggt
     3601 ggagcagctc gagttcccgg tgctctacac cgacgcgcgc aagggcatcg ccaagcggtc
     3661 gttgaccgac gagtcgaaga ccctggcccc gctcttcgag accctgctcg agacgatccc
     3721 ggcgcccaag ttcgaggagg gtcacgggct ccagctccgg gccgcctcgc tcgactggga
     3781 cgactacgtg gggcggctga tcatcggccg catcgtcaac gggcgcgtgc gccagtacga
     3841 ccgggtcgcg gtggtccacc gcgacggcac catcgagaac gcgaagatca ccgtgctcta
     3901 cggctacgag ggtctcaagc gcgtggaagt acctgagatg tccgcggggg acatcgtggc
     3961 ggtggcgggc atcgaggcca tggagatcgg cgagaccatc gcggatcccg agaagccgat
     4021 cgcgctgccg gtcatgcaca tcgacgagcc caccgtggcc atgctcttct cctccaacgt
     4081 ctcgccgttt gcgggcaagg agggcaagtt cgtgacctcg acccagctcc gcgagcggct
     4141 ctggcgggag aagcggagca acgttgcgct gcaggtggac gagaccgatt cgcccgacac
     4201 cttcaaggtc tccgggcgcg gcgagttgca gctcgcgatc ctcatcgaga tgatgcggcg
     4261 cgaaggctac gagatggagg tctccaagcc ccagatcatc acccacgagg agaacggcaa
     4321 gaccctcgag ccgatggagc acctggtggt ggacgtgccg gacgacttca tcggcgcggt
     4381 cacccagaag ctcgggcccc ggaagggcca gatggtgaag atggtcaacc acggcaccgg
     4441 ccgggtgcgg ctcgagtacc gcatcccgtc tcgcgggttg atcggctacc gctccgagtt
     4501 cctcaccgac acccgcggca ccgggctgct caaccacctc ttcgacggct acgcggactg
     4561 gcagggcgac atcccccacc gggcgagcgg ggcgatggtg gccgaccgca cgggcaagac
     4621 caccgcgtac gcgatcgacc acctccagcc gcgcggcatc ctctttctgg gccccggcga
     4681 gccggtctac gaaggccagg tggtgggcga gaacgcgcgc gacaacgacc tcgacgtcaa
     4741 catcaccaag gagaagaagc tcaccaacgt gcgctcgtcc acctcggacg aggcggtgcg
     4801 cctcactcct ccgcacctga tgaacctcga gcagtcgatc gagtggatcc gcgaggacga
     4861 gctcctcgag atcaccccga agtcgctccg cctccgcaag aaatccctca gcggccgccg
     4921 ccgcttctag ctcggcgcag ccggagcgaa agcgccgctc atcgacgcca tcgctccggc
     4981 tcttctcgcg cctactgctt catcgcggcc atgcggcggg ccatctcttc catgctcacg
     5041 tcttccttgt gagtggagag gtgccaggtg tggccgaagg gatccttcag ggtgccggag
     5101 cggtcgccgt agaactggtc cgagaccggg cgcacctcgg tggcgccggc ggcgagggcc
     5161 tgcctgaaca tggcgtccac gtcggtcacg tacagcagga ggctgaccgg agagcccccg
     5221 aagtgcttgg gcgcgtacgc gtccatctcg ggatgctcgt cggccatcat gagcaccgag
     5281 tcgcccacct tgatctcggc atggccgatc cgttcgttgg gcgccgggat gcgcaacagc
     5341 tccttcgcgt tgaaggcccg ctggtagaac tcgatcg
//
LOCUS       DATMCV010000659         7715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_32284, whole genome shotgun sequence.
ACCESSION   DATMCV010000659 DATMCV010000000
VERSION     DATMCV010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32284"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VNU03_15115"
     CDS             complement(<1..551)
                     /locus_tag="VNU03_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HWP75850.1"
                     /translation="MASLKDKYCIVGVGETEYSRQSARSTRAMAVEAVRNAMRDAGLG
                     KDGVDGMMSYQSGDSVFSNFVAPDLGIRLNFYMDVFGGGSSTEALVGLAMGAIEAGMC
                     HTVAIFRSMNGYSDFRIGGTGARAAQPVRGLDLAQVPFGMRSAGQNFAPTFMRHMYEY
                     GTTSEQVAHVRVAHSKHASQNPKA"
     gene            complement(630..1256)
                     /locus_tag="VNU03_15120"
     CDS             complement(630..1256)
                     /locus_tag="VNU03_15120"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75851.1"
                     /translation="MRPGRPAEDWTIGRALRSRQFWILYGVFTATWAPVFIPLVHLVP
                     MARGLGVPPLLAATLVSALGAAALIGRLVMGGASDRIGRRGALAIALVLQTGAFVGFA
                     QSHTLPALYASSLVFGFSYGAASTLFPATVADAFGREQAGTLAGLLFALAGSVAALGP
                     VVAGFIYDRAGDYQLAWWLSAGCNGLALLLLASFRVPVARLADDRAVA"
     gene            complement(1319..1897)
                     /locus_tag="VNU03_15125"
     CDS             complement(1319..1897)
                     /locus_tag="VNU03_15125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75852.1"
                     /translation="MRAASPGAPPGHVFYGWTVVTAAGLILFMAYGTQYAFGVFFAAL
                     VEEFGWSRASLSGVFSLYAAVYSGFALVAGRLTDRWGPRTVIAIGGALLGAGLVAMSR
                     VGALWQPYLLYGTIAALGMSTAYVPCNATVAKWFTRRRGLAVGLASAGGSLGTLALPP
                     VAHFLVSRLGWRWSYVVFGLAIFLTLNALAVI"
     gene            complement(1884..3143)
                     /locus_tag="VNU03_15130"
     CDS             complement(1884..3143)
                     /locus_tag="VNU03_15130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HWP75853.1"
                     /translation="MDYHHLLEATGTVVSGLLFYSLAYGWFAPDDPRRRPLWIAVMGL
                     AWGGLAIILMISRIQTVEGVFVDGRVVPVAIIGLFEGGWAGLIAALTASAYRIWLGGS
                     GAAAGVITLLAVAAAAGLVHRWAAVAEKVRFRHAFVLAVATFFITFGGFGLLGERGRT
                     LFARVWPSYLALIAVGIPAIALLMASIVDRRALAQERERFRAVLDDATDAVRVVEVGA
                     QRIIDCNRADCDLSGLSRAQLVGRDNREFWPSPAADAAPSGPPGGATGDGSIARALGV
                     PFQTASGRLLAVDSSRRVVEYRGRRYEIIIYRDAAERLAGEEARREAASLRSVNLLAQ
                     AAAHEINNPLAIIMGYVQMLEDRLPPGGEESGWAHTCKNAAVRIRDAVGRLSRIVRVE
                     STHPTGTIPPILDMERSTTKRPNDAGR"
     gene            complement(3143..5950)
                     /gene="uvrA"
                     /locus_tag="VNU03_15135"
     CDS             complement(3143..5950)
                     /gene="uvrA"
                     /locus_tag="VNU03_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWP75854.1"
                     /translation="MSQPVWLRIEGARQNNLKNVSVDIPHDRLTVITGVSGSGKSSLA
                     FDTLFAEGQWRYIESLSTYARMFLERLDRPDVDRIEHIRPAIALEQKNPVRTARSTVG
                     TATELHDYLRLLFAKIGRVHCPTCGAEARSDSADQVADALLRDHPGARGMICFPLPAE
                     PARDPRALFATLLQRGFARVKAGDLVLDLAEVQSRPDRIPADLDPARLAVVLDRVVVG
                     PDSRRRITDSLETALAEGEGRASVDLLERGVVPVSRDFHCPACGVALSRPQPLLFSFN
                     HPLGACPECKGFGNVLRYDEARVVPDQTKSLADGAVEPWSHPSGRWYQKQLLKAAKKR
                     GVDATVPYAALPEEDRRWVYDGGGGLTGIRGFFEEVESYRYKLHVRVFLSRYRSQSPC
                     PRCEGRRLRPEALAVRVAGASVADFGLKTVEELDALFATLSLTEWEATVARDVLKLLR
                     AKLSFLLRVGLGYLTLARQTRTLSGGEAQRINLANQLGAQLTGTLYVLDEPSIGLHPR
                     DTARLAELCRELASGGNTVVMVEHDRALIEVADYLIEMGPGSGERGGHVVFAGTQDEF
                     RKDPRSLTARYMSGRDTIALPLSRRPGRRALVLAGARANNLKNITVKIPLNTLTCVTG
                     VSGSGKSTLVHDTLYRAVARHFKADFAVPGAHDELRGLQHLKGIRLIDQEPIGRTPRS
                     NPVTYVKAFDEIRRLFAALPRAHTLGLGPGAFSFNVPGGRCDTCEGDGFQKLEMYFVE
                     DVYVTCQECEGKRYRSDVLQVTYKNRDISQVLHLTVDEAVDFFASEPVLQRRLKILRE
                     VGLGYLRLGQPATTLSGGEAQRLKIAAELSARLTTDHLFILDEPTTGLHLDDIRKLLL
                     VLNRLVDAGNTVLVVEHHLDVIKTADWVVDLGPEGGEAGGELVAEGTPEQIAQASGSY
                     TGKFLRDLLPRPNGRSASGRG"
     gene            6121..6570
                     /locus_tag="VNU03_15140"
     CDS             6121..6570
                     /locus_tag="VNU03_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HWP75855.1"
                     /translation="MEATLRARGLRLTGPRRLVLDVVRATESHPTAEWVHRMVRRRRP
                     RVSLGTVYRNLRLLVAQGLVKELPGPHARFDGNVSEHHHFICLACGSISDVPGPATEP
                     HSRALCGRVAAQSGFSVTHHRIEFYGRCLECQRRAGRKSARSRRPAP"
     gene            6627..7076
                     /locus_tag="VNU03_15145"
     CDS             6627..7076
                     /locus_tag="VNU03_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HWP75856.1"
                     /translation="MAGKSLKGSKSVDNLKDAFAGESQANRRYLYFARVADIEGFPDI
                     AGLFKDTADAETGHAFGHLDFLKEVGDPATGEPIGKTEKNLKASVAGETYEYTQMYPG
                     MAKTARDEGYPELAEWFETLAKAEKSHAGRFTKGLNQVAGKDPADAI"
     gene            7164..>7715
                     /locus_tag="VNU03_15150"
     CDS             7164..>7715
                     /locus_tag="VNU03_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWP75857.1"
                     /translation="MTLDIRAPEFWRLDRVDTELRRVYDICAGCRRCLPLCPSFKVMF
                     DRLDVEAVDGDVEKLPAADVKEVVDLCYQCKLCYNHCPYTPPHRWAVDFPRLMLRARS
                     AEARDKGVTLQDRFLGNTELVGRLGSLTAPISNWMNAVWVNRKFMEMAVGIHARRDLP
                     AFHRETFSRWFDGRRPVARREPAR"
BASE COUNT         1185 a         2716 c         2811 g         1003 t
ORIGIN      
        1 gccttcgggt tctgcgaggc gtgcttgctg tgggccaccc gcacgtgggc cacctgctcg
       61 gaggtggtgc cgtactcgta catgtggcgc atgaaggtcg gcgcgaagtt ctgccccgcg
      121 ctgcgcatgc cgaacggcac ctgggccagg tcgagcccgc gcaccggctg ggccgcgcgg
      181 gcgccggtgc cgccgatgcg gaagtcggaa tagccgttca tggagcggaa gatcgctacc
      241 gtgtggcaca tgccggcctc gatggcgccc atcgcgaggc cgaccagggc ctcggtggag
      301 gagccgccgc cgaagacgtc catgtagaag ttgagccgga tgcccaggtc gggcgccacg
      361 aagttggaga agacggagtc gccgctctgg taggacatca tgccgtccac gccgtccttg
      421 ccgaggccgg cgtcccgcat ggcgttgcgc accgcctcga ccgccatcgc gcgggtgctg
      481 cgcgcagact gtcggctgta ctcggtctct ccgaccccca cgatgcagta cttgtccttg
      541 agagatgcca tggagcgctc ctcgacgatg accgcgtgac gatcgaccgg atcaggccgc
      601 gccactgtac gcgagcgcgg cggggggcgt caagcgaccg ctcgatcgtc cgccaagcgc
      661 gcgaccggga cccggaagga ggcaagcagc agcagcgcga gcccgttgca gcccgcgctg
      721 agccaccagg ccagctggta gtcgcccgcg cggtcgtaga tgaagccggc gaccaccggg
      781 ccgagcgcgg ccacggagcc ggcgagggcg aagaggaggc ccgcgagggt gcccgcctgc
      841 tcgcgcccaa aggcgtcggc caccgtggcc gggaagaggg tcgaggccgc cccgtaggag
      901 aagccgaaca cgagcgagct cgcgtagagc gcgggcaggg tgtgcgactg cgcgaacccc
      961 acgaaggcac ccgtctgcag aacgagcgcg atcgcgaggg cacctcgccg tccgatgcgg
     1021 tccgacgccc cgcccatgac cagacggccg atcagcgcgg cggcgccaag tgcgctcacc
     1081 agcgtggccg cgaggagggg cgggacgccg agcccccgcg ccatcgggac caggtgcacg
     1141 agcgggatga agaccggggc ccaggtggcg gtgaacaccc cgtagaggat ccagaactgg
     1201 cgcgaccgga gcgcgcggcc gatagtccag tcttcggccg gccgtccggg gcgcaccggc
     1261 cctcgccccg ggcggtcgcc gtccggggcg aggccgacgg tctcgggatc ccgccgcatc
     1321 atatcaccgc gagcgcgttc aacgtcagaa agatcgcgag gccgaacacc acgtaggacc
     1381 agcgccagcc gagtcggctc accaggaagt gggccaccgg cgggagcgcg agcgtgccga
     1441 ggctgccgcc cgcgctggcc agccccaccg cgagcccgcg ccgacgtgtg aaccacttgg
     1501 ccacggtggc gttgcacggc acgtaggcgg tggacatccc gagggccgcg atcgtgccgt
     1561 agagcaggta gggctgccag agcgcgccga cccggctcat cgccacgagg ccggcgccga
     1621 gcagcgcgcc gccgatcgcg atgacggtgc gcgggcccca ccgatcggtg agccggcccg
     1681 ccaccagggc gaagcccgag tagactgccg cgtagagcga gaagacgccc gagaggctcg
     1741 cgcggctcca gccgaactcc tccacgagcg ccgcgaagaa gaccccgaag gcgtactggg
     1801 tgccgtaggc catgaacagg atgaggcccg ccgcggtcac caccgtccag ccgtagaaga
     1861 cgtgtcccgg cggggcgccg ggactagcgg cccgcatcgt tggggcgctt ggtggtggac
     1921 cgctccatgt cgaggatcgg tgggatggta ccggtgggat gcgtgctctc gacccggacg
     1981 atgcggctca gccggccgac cgcgtcccgg atgcgcaccg ccgcgttctt gcaggtgtgc
     2041 gcccagccgc tctcctcgcc gccggggggc aggcgatcct ccagcatctg cacgtagccc
     2101 atgatgatgg cgagcggatt gttgatctcg tgggcggcgg cctgggcgag caggttgacc
     2161 gagcggagcg aggccgcttc ccggcgggcc tcttccccgg ccaggcgctc ggccgcgtcg
     2221 cggtagatga tgatctcgta gcgccggccg cgatactcga cgacgcgccg gctcgagtcg
     2281 accgcgagca gtcgcccgga ggcggtctgg aatggcactc cgagggcccg cgcgatcgag
     2341 ccgtcccccg tcgcgccgcc gggcgggccg ctgggggccg cgtccgcggc cgggctcggc
     2401 cagaactccc ggttgtcgcg gcccaccagc tgcgcccgcg acagcccgga caggtcgcag
     2461 tcggcgcggt tgcagtcgat gatccgctgg gcgcccacct ccaccacccg gaccgcgtcg
     2521 gtggcgtcgt cgagcaccgc gcgaaagcgc tcccgctcct gcgcgagggc ccgccggtcg
     2581 acgatactcg ccatcaggag cgcgatggcc gggatcccga ctgcgatcag ggccaggtag
     2641 gatggccaca cgcgcgcgaa cagcgtgcga ccgcgctccc cgagaaggcc gaagccgccg
     2701 aaggtgatga agaacgtggc caccgccagc acgaacgcgt gccggaagcg caccttctcg
     2761 gccaccgcgg cccagcggtg gacgaggccc gcggcggccg ccaccgcgag cagggtgatc
     2821 acgcccgccg ccgctccgct cccgccgagc cagatccggt acgccgacgc ggtgagcgcc
     2881 gcgatgaggc ccgcccagcc cccctcgaac agcccgatga tcgccaccgg gaccacgcgg
     2941 ccgtccacga agacgccctc gacggtctgg atgcgggaga tcatgagaat gatcgcgagg
     3001 ccgccccacg ccaggcccat caccgcgatc cagagcgggc gccggcgcgg atcgtccggg
     3061 gcgaaccagc cgtaggcgag cgagtagaac aggaggcccg agaccaccgt gccggtcgcc
     3121 tccagcaggt ggtggtagtc catcaccccc gcccggacgc ggagcggccg ttcggccggg
     3181 gcaggagatc gcggaggaac ttgccggtgt aggagccgct cgcctgcgcg atctgctcgg
     3241 gcgtgccctc ggcgaccagc tccccgcccg cctcgccacc ctcggggccc aggtccacca
     3301 cccagtcggc ggtcttgatc acgtcgaggt ggtgctcgac caccagcacc gtgttgcccg
     3361 cgtcgaccag gcggttcagg acgaggagca gcttgcggat gtcgtcgagg tgcagcccgg
     3421 tggtcggctc gtccaggatg aagaggtggt cggtggtgag ccgcgcggac agctcggccg
     3481 cgatcttgag gcgctgggcc tcgccgccgg acagggtggt ggcgggctgg cccagtcgca
     3541 ggtagccgag ccccacctcg cgcaggatct tgagccgccg ctgcagcacc ggctcggacg
     3601 cgaagaagtc gaccgcctcg tccaccgtga gatggaggac ctggctgatg tcgcggttct
     3661 tgtaggtgac ctgcaggacg tcggagcggt accgcttccc ctcgcactcc tggcaggtca
     3721 cgtacacgtc ctccacgaag tacatctcga gcttctggaa gccgtcgccc tcgcaggtgt
     3781 cgcagcgccc gcccggcacg ttgaacgaga aggcgccggg tccgagtccc agggtgtggg
     3841 cgcggggcag ggccgcgaag agccggcgga tctcgtcgaa ggccttcacg taggtgaccg
     3901 ggttggagcg cggggtgcgc ccgatgggct cctggtcgat gagccggatc cccttcaggt
     3961 gctgcagccc gcgcagctcg tcgtgggcgc ccggcaccgc gaagtccgcc ttgaagtggc
     4021 gcgccaccgc ccggtagagg gtgtcgtgca ccagggtgga cttcccggag cccgagaccc
     4081 cggtgacgca ggtgagggtg ttgagcggga tcttcacggt gatgttcttc aggttgttcg
     4141 cgcgcgcgcc cgccagcacg agggcgcgac ggcccggccg ccggctgagc ggcagggcga
     4201 tcgtgtcgcg gccgctcatg tagcgcgcgg tcagcgagcg cggatccttg cggaactcgt
     4261 cctgggttcc cgcgaagacg acgtggccgc ctcgctcgcc cgagccgggg cccatctcga
     4321 tcaggtagtc ggccacctcg atgagggcgc gatcgtgctc gaccatcacc accgtgttgc
     4381 cgccggaggc gagctcgcgg cagagctcgg cgagccgggc ggtgtcccgc gggtgcaggc
     4441 cgatcgacgg ctcatccagc acgtagaggg taccggtgag ctgggccccg agctgattgg
     4501 cgaggttgat gcgctgcgct tcgccgcccg acagggtgcg cgtctgccgc gcgagggtga
     4561 ggtagccgag gccgacccgg agcaggaacg agagcttggc gcgcagcagc ttgagcacgt
     4621 cgcgcgccac ggtggcctcc cactcggtga gggagagggt cgcgaagagc gcgtcgagct
     4681 cctccaccgt cttgagcccg aagtcggcga cgctggcccc cgccacccgc accgcgaggg
     4741 cctccggccg cagccgccgg ccctcgcagc gcgggcaggg cgactggctg cggtagcgcg
     4801 agaggaagac ccggacgtgc agcttgtacc ggtaggactc gacctcctcg aagaagccgc
     4861 ggatcccggt gagcccgccg ccgccgtcgt agacccagcg gcgatcctcc tcgggcagcg
     4921 ccgcgtacgg caccgtcgcg tcgaccccgc gcttcttggc cgccttgagg agctgcttct
     4981 ggtaccagcg gcccgagggg tgggaccacg gttccaccgc gccgtcggcc aggctcttgg
     5041 tctgatccgg caccacccgg gcctcgtcgt accgcagcac gttgccgaag cccttgcact
     5101 ccgggcaggc gccgagcggg tggttgaacg agaagagcag cggctggggg cgactcaggg
     5161 cgaccccgca ggccgggcag tggaagtcgc ggctgaccgg caccacgccg cgctcgagca
     5221 gatccaccga ggcgcggccc tcgccctcgg ccagcgcggt ctcgagcgag tcggtgatgc
     5281 gccgccggct gtccgggccg accaccaccc ggtccagcac caccgcgaga cgcgcgggat
     5341 cgagatcggc ggggatgcgg tccggccggc tctggacctc ggccaggtcc aggaccaggt
     5401 cgcccgcctt cacgcgcgcg aagccgcgct ggagcagggt cgcgaagagc gcgcggggat
     5461 cgcgggcggg ctccgcgggc agggggaagc agatcattcc ccgggcgccg gggtgatcgc
     5521 gcagcagggc gtcggccacc tggtcggcgg agtcgctccg cgcctcggcc ccgcaggtcg
     5581 ggcagtggac ccggccgatc ttggcgaaga ggagacggag gtagtcgtgc agctcggtcg
     5641 cggtgcccac ggtggaccgc gcggtgcgca ccgggttctt ctgctcgagc gcgatggcgg
     5701 gacggatgtg ctcgatccgg tccacgtcgg gccggtcgag ccgctccagg aacatgcgcg
     5761 cgtacgtcga gagcgactcg atgtagcgcc actggccttc cgcgaagagg gtgtcgaacg
     5821 cgagggacga cttgcccgac cccgagaccc cggtgatcac ggtgagccgg tcgtggggga
     5881 tgtcgacgga gacgttcttc aggttgttct gccgggctcc ctcgatgcgg agccacaccg
     5941 gctgggacat tcgagcggtc ctcctgagat ggaaagtcta ctcgacgcgc cgagggcctt
     6001 caatagcgag cgtcccgagc gcgcgtcccg cttgacagcg gcccgccggg ccccgtatcc
     6061 ttcagaaccg taatgagaac cattcctaaa aatcgcgagc gaccccggca agaggccggg
     6121 gtggaggcaa ccctccgggc gcgcggattg cgcctcaccg ggccgcgccg gctggtgctc
     6181 gacgtggtcc gcgccaccga gtcgcatccc accgcggagt gggtgcaccg gatggtgcga
     6241 cgccggcgcc cgcgggtgag cctgggcacc gtctaccgca acctgcggct gctcgtggcg
     6301 caggggctgg tcaaggagct gcccggcccc cacgcgcggt tcgacggcaa cgtgagcgag
     6361 caccaccact tcatctgcct cgcctgcggc tccatcagcg acgtgccggg gccggccacc
     6421 gagccccact cccgcgccct ttgcggccgg gtggccgccc agagcggctt cagcgtcacg
     6481 caccaccgga tcgagttcta cggtcgctgt ctcgagtgcc agcgccgcgc ggggcggaag
     6541 agcgcgcgga gccggcgccc cgcgccgtag cccgggggcc gtccccggcc gccgtcacta
     6601 ccaactgctg cagaggagga catcacatgg caggcaagtc cctcaagggc tcgaagagcg
     6661 tggacaacct caaggacgcg ttcgcgggcg agtcgcaagc caaccgccgc tacctgtact
     6721 tcgcgcgcgt cgccgacatc gaaggcttcc ccgacatcgc gggcctgttc aaggacaccg
     6781 cggacgcgga gaccgggcac gccttcggtc atctcgactt cctcaaggaa gtgggcgatc
     6841 cggccaccgg cgagcccatc ggcaagaccg agaagaacct gaaggcctcg gtcgcgggcg
     6901 agacctacga gtacacccag atgtacccgg gcatggccaa gactgcgcgg gacgagggct
     6961 atccggagct ggccgagtgg ttcgagaccc tcgccaaggc cgagaagtcg cacgcgggcc
     7021 gcttcaccaa ggggctcaac caggtcgcgg gcaaggaccc ggccgacgcc atctagatcc
     7081 cggccggatc gatcgggcgc cggccgaccg gccggcgccc ggtccgaggc ctccaaccgc
     7141 ccagtgcgtc aggcgagagg acgatgacac tcgacattcg cgcacccgaa ttctggcggc
     7201 tcgaccgcgt ggacaccgag ctccgccggg tctacgacat ctgcgcggga tgccgccgct
     7261 gcctgcctct ctgcccctcg ttcaaggtca tgttcgatcg gctggacgtg gaggcggtgg
     7321 acggcgatgt cgagaagctg cccgccgccg acgtgaagga agtggtcgat ctctgctacc
     7381 agtgcaagct ctgttacaac cactgcccgt acaccccgcc gcaccggtgg gcggtggact
     7441 tcccccgcct catgctgcgg gcgcgctccg cagaggcccg cgacaagggc gtgacccttc
     7501 aggatcgctt cctcggcaac accgagctgg tcgggcgcct gggcagcctg accgcgccga
     7561 tctcgaactg gatgaacgcg gtgtgggtca atcgcaagtt catggagatg gcggtcggca
     7621 tccacgcccg gcgcgacctg cccgcgttcc accgcgagac gttctcccgc tggttcgacg
     7681 ggcggcgacc cgtcgcgcgg cgggaaccgg cccgg
//
LOCUS       DATMCV010000660         3948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_169521, whole genome shotgun sequence.
ACCESSION   DATMCV010000660 DATMCV010000000
VERSION     DATMCV010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169521"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VNU03_15155"
     CDS             <1..1511
                     /locus_tag="VNU03_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP75858.1"
                     /translation="LFVVIPKGFFPQQDNGRLSGGVVASQDISFQAMREKVTQLAAIV
                     QADPGVLTVTAYTGGGGGRGTTVNTARMFVALKPSKERDVTADQIIARLRPKLARVVG
                     ATLYLQAVQDIRVGGRIGNAQYQYTLQADSLKDLSEWAPRMLEALRALPELRDVSSDQ
                     QDAGLQVPLTIDRDTAARLGVSTKLIDDTLYSAFGQRQVSTIYTSLNQYHVVMEVAPE
                     FWQSPDALSAIYVRSQTGATVPLTALTRFQPSAAPLQVNHQGLFPSVTVSFNLAPDVS
                     LGHAVTAVEDAGRRIALPSSVRGKFAGAAQAFQDSLSTQPLLILAALVAVYIVLGMLY
                     ESYVHPLTILSTLPSAGVGALLALMICRMDLSIIAIIGIILLIGIVKKNAIMVIDVAL
                     DLERREGRSPRDAIYTACLQRLRPILMTTMAAMLGALPLALGTGTGSELRRPLGVSII
                     GGLMLSQLLTLYTTPVVYLFLERWRMRTAGAWARVGERLGVRRPAEQISGGK"
     gene            1538..2155
                     /locus_tag="VNU03_15160"
     CDS             1538..2155
                     /locus_tag="VNU03_15160"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWP75859.1"
                     /translation="MQLDPRTMKQESTYKLLIGCVVPRPIAWVSTVGPDGVNNLAPFS
                     FFMGVCGDPPTIAFSSGPRAKDRKDTVRNAEHTGDFVVNVVDDDRAEQMNQSSGEYPA
                     EVDEFALTGLTAAPGVRVRSPRVVEAPISLECRVAQIIPVGHGPHSLVLGEILYFHVR
                     DDLYEAATGRIDMARLKPVGRLAGHAYAHVHDIFQMKRPSETYAG"
     gene            complement(2152..3696)
                     /locus_tag="VNU03_15165"
     CDS             complement(2152..3696)
                     /locus_tag="VNU03_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP75860.1"
                     /translation="MTDPAKQPPGRAVGEGERLYRILAEHSSDVIAVFDLQSRPLYVS
                     PSVFQLRGYTPEECLAQTTEERLTPRSQELVRQALAEVLAVERAGETPPNSYRRLELE
                     QPCKDGSTVWVESTVTWIRDEAGKPTGVLSMTRDITARRRAEAALAESQVQLRESQKL
                     EAVGRLAGGVAHEFNNLMTVVLGRSQWLLTQHGDDATLRPQLEMIERAGQRAAHLTAQ
                     LMAFCRRQSHAPVVVDLNEIVRAVTPALRRLLPAPIELATRLHEGLPPVEADRLQLEH
                     VLHQLVMNARDATPDGGRVILETSATGREGGARTIVLSVADTGIGMSEEVRSHAFEPF
                     FTTKDIGAGSGLGLAMVYGSVQQHGGRVEMDSVPGVGTTVRIHLPALAPAAPALPPRA
                     TVLVVERDPAAAERLAEVLGSGGYRVLPARGGEEALEVAARHPGPIDLLITEVMLGGL
                     NGAELSARLRRARPGLRTLFVIEGAGEPDPRLPRQGPEDWLVPRRFSAASLLEKVAHA
                     LPPADG"
BASE COUNT          559 a         1438 c         1377 g          574 t
ORIGIN      
        1 acctcttcgt ggtgatcccc aagggcttct tcccccagca ggacaacggc cggctcagcg
       61 gcggggtggt ggcctcccag gacatctcgt tccaggcgat gcgcgagaag gtgacccagc
      121 tcgccgcgat cgtgcaggcc gaccccggcg tcctgaccgt caccgcctac accggcggcg
      181 gcgggggccg gggcaccacc gtcaacaccg cgcgcatgtt cgtggccctc aagccgagca
      241 aggagcggga cgtcaccgcg gaccagatca tcgcgcgcct ccgtccgaag ctcgcccgcg
      301 tggtcggggc caccctctac ctgcaggcgg tccaggacat ccgggtcggc ggccgcatcg
      361 gcaacgccca gtaccagtac acgctccagg cggacagcct gaaggatctg agcgagtggg
      421 ccccgcgcat gctcgaggcg ctccgcgcgc ttcccgagct gcgcgacgtc tcgagcgacc
      481 agcaggacgc cgggctccag gtgccgctca ccatcgatcg cgacaccgcg gcccggctcg
      541 gggtcagcac caagctcatc gacgataccc tctacagcgc gttcggccag cgccaggtct
      601 cgaccatcta cacctcgctc aaccagtacc acgtggtgat ggaggtggcg cccgagttct
      661 ggcagagccc ggacgcgctc tccgccatct acgtgcgctc ccagaccggc gccacggtgc
      721 ccctcaccgc gctgacccgc ttccagccga gcgcggcccc actccaggtg aaccaccagg
      781 gccttttccc ctcggtgacc gtgtcgttca acctggctcc cgacgtctcg ctcggccacg
      841 cggtgaccgc ggtggaggac gcggggcgcc gcatcgccct gccctcgagc gtgcgcggca
      901 agttcgcggg ggcggcccag gccttccagg actcgctctc cacccagccc ctgctgatcc
      961 tggccgccct cgtcgcggtc tacatcgtgc tcggcatgct ctacgagagc tacgtgcacc
     1021 cgctgaccat cctctccacc ctgccgtcgg cgggagtggg cgcgctgctg gccctgatga
     1081 tctgccggat ggacctgtcg atcatcgcga tcatcgggat catcctgctg atcggtatcg
     1141 tcaagaagaa cgcgatcatg gtcatcgacg tcgcgctcga tctcgagcgg cgcgagggcc
     1201 ggtcgccgcg cgacgccatc tacaccgcgt gcctgcagcg gctgcgcccc atcctcatga
     1261 ccaccatggc cgcgatgctg ggggccctgc cgctcgccct cggcaccggc accggctccg
     1321 agctccggcg gccgctcggg gtgagcatca tcggcgggct catgctgagc cagctcctca
     1381 cgctctacac gaccccggtg gtgtacctct tcctcgagcg ctggcggatg cggaccgcgg
     1441 gggcgtgggc gcgggtcggc gagcggctcg gggtgcgccg gcccgccgag cagatcagcg
     1501 gcggcaaata accgatctcg aacgagagga gtcccgcatg cagctcgatc cccggacgat
     1561 gaaacaggag tccacctaca agctgctcat cggctgcgtg gtgccgcggc cgatcgcgtg
     1621 ggtctccacc gtcggccccg acggggtgaa caacctcgct cccttcagct tcttcatggg
     1681 cgtgtgcgga gacccgccca ccatcgcctt ctcctcgggc ccgcgcgcga aggaccgcaa
     1741 ggacaccgtg cgcaacgccg agcacaccgg cgacttcgtg gtcaacgtgg tggacgacga
     1801 ccgggccgag cagatgaacc agagctccgg ggagtatccc gccgaggtgg acgagttcgc
     1861 gctcaccggg ctcaccgccg cgcccggggt gcgggtgcgc tcaccgcggg tggtggaggc
     1921 gccgatcagc ctcgagtgcc gggtggccca gatcatcccg gtcgggcacg ggccccacag
     1981 cctcgtcctg ggcgagatcc tctacttcca cgtgcgcgac gatctctacg aggccgcgac
     2041 cgggcgcatc gacatggcgc gcctcaagcc ggtgggccgt ctcgccgggc acgcctacgc
     2101 gcacgtgcac gacatcttcc agatgaagcg gccgtcggag acctacgcgg gctagccgtc
     2161 cgcgggcggc agcgcgtggg cgaccttctc gaggaggctc gcggccgaga atcgccgggg
     2221 cacgagccag tcctcggggc cctggcgggg cagccgcggg tcgggctcgc ccgcgccctc
     2281 gatcacgaac agggtgcgga ggccgggccg ggcccgccgg aggcgcgcgg atagctcggc
     2341 gccgttgagg ccacccagca tcacctcggt gatcagcagg tcgatcggcc cggggtgccg
     2401 cgcggcgacc tcgagcgcct cctcgccgcc tcgcgcgggc agcacgcggt agccgccgct
     2461 ccccagcacc tcggccaggc gctccgcggc cgcggggtcg cgctcgacca ccagcacggt
     2521 ggcgcgcgga ggaagcgcgg gcgcggcggg cgccagcgcg ggcaggtgga tccgcacggt
     2581 ggtgccgacc ccgggcacgc tgtccatctc gacgcgcccg ccgtgctgct gcacggagcc
     2641 gtacaccatc gccagcccga ggccgctgcc cgccccgatg tccttggtgg tgaagaaggg
     2701 ctcgaaggcg tggctgcgca cctcctcgct catcccgatg ccggtgtcgg ccaccgagag
     2761 cacgatcgtg cgcgcgccgc cctcccgccc cgtggcgctg gtctcgagga tgacgcggcc
     2821 gccgtccggc gtcgcgtcgc gcgcgttcat caccagctgg tggagcacgt gctcgagctg
     2881 caggcggtcc gcctccaccg ggggcaggcc ctcgtgcaac cgggtcgcca gctcgatcgg
     2941 cgcggggagc aggcgacgga gcgcgggggt gaccgcccgg acgatctcgt tcaggtccac
     3001 caccaccggg gcgtgggact ggcgccggca gaaggccatg agctgggcgg tgagatgcgc
     3061 ggcgcgctgg ccggcccgct cgatcatctc gagctggggc cgcaaggtcg cgtcgtcgcc
     3121 gtgctgggtg agcagccact gcgagcgccc gagcaccacc gtcatgaggt tgttgaactc
     3181 gtgggccacg cctcccgcca gccgtccgac cgcctcgagc ttctgtgact cgcgcagctg
     3241 gacctggctc tcggccagcg cggcctcggc ccgccggcgc gcggtgatgt cgcgggtcat
     3301 gctgagcacg ccggtgggct tgcccgcctc gtcgcggatc caggtgaccg tggattccac
     3361 ccacaccgtc gacccgtcct tgcagggctg ctccagctcg agccggcgat acgagttggg
     3421 cggcgtctcg cccgcccgct ccaccgcgag cacctcggcg agggcctgcc gcaccagctc
     3481 ctgcgagcgc ggggtgaggc gctcctcggt ggtctgggcg agacattcct ccggcgtgta
     3541 gccacggagc tggaagacgg acgggctcac gtagagaggt cgcgattgca ggtcgaagac
     3601 cgcgatgacg tcggaggagt gctcggccag gatccggtag aggcgctcgc cctcccccac
     3661 cgcgcgccca gggggctgct tcgcgggatc cgtcatggtc gaattccacg atagcacggg
     3721 ggcttggggt ggacgaatga ggggtcggtt cggttgaccg gcacgaagat ttgccgcact
     3781 gggtcatggt caaccgaagg gcgaatccac tcgtccccat aaatgaatgg gccgcgactg
     3841 ccgtcagaga cggtggccct cgaagcgggc cctcttgagg aggaacgtga tcatgcgaat
     3901 gcgcgtgctt ggcccgatgg tgctggtcgg tggcctggtc gctctgtt
//
LOCUS       DATMCV010000661         2540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_233983, whole genome shotgun sequence.
ACCESSION   DATMCV010000661 DATMCV010000000
VERSION     DATMCV010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233983"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VNU03_15170"
     CDS             complement(<1..269)
                     /locus_tag="VNU03_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75861.1"
                     /translation="MSPFLAQLAELCRTERTRAKWVIVPTHTLGHTLGERLALAETGW
                     ANLRFATPLDLALPMAAPFLVERGVDPAPDGIGPALIMRLLLELPA"
     gene            complement(361..963)
                     /locus_tag="VNU03_15175"
     CDS             complement(361..963)
                     /locus_tag="VNU03_15175"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWP75862.1"
                     /translation="MLQELLQRFTYLGILAALLLGSVGVPIPEEMPIVAAGILSHEGL
                     ARWWLALPVCVLGVLAGDVVLYWAGRRWGERLLRWRLVRHVLTRARADWLKAAYREHA
                     MKTVAMARHVTGLRAAAFLTAGIARVPFWKFILADAAAAAVTVPLAFGLAYFFTHQIN
                     AILADVHRVERWLALAGLVAVAGALVVYSVRGNRRVPKNS"
     gene            1146..2240
                     /locus_tag="VNU03_15180"
     CDS             1146..2240
                     /locus_tag="VNU03_15180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWP75863.1"
                     /translation="MRHLTLPRAASTILALLFLLAPAVARAQLAPSYSQDDQLANEPA
                     PWTVPGKWGPRGNWGRWGEQDKRGMLNFLTPDLIVKAAGLVKQGKVYALGEEMHSDLP
                     RAITPSRAGVQIIHENDGYDRVTQPGQFDPKRNQGAASFTIMHNHVGTHLDTFAHIYR
                     ENSLFNNMPPPRPVGTVHGDAASVKFIVGRGVLLDVAKYKGTDPLPVNYWITTADLQN
                     TAKAQGVEIRKGDVLLIRTGWRKMWDEPGPDGRVDLSHAKYLQPEPGVGPDTLRFLND
                     MEIVAIGADTAAVEWNFPLQAGYSRKAFGGFQGLPLHVDFIWNRGAYILEIMNLDELA
                     KDQAYEFLFVVAPLLLRGGIGVPVNPIAIR"
     gene            2250..>2540
                     /locus_tag="VNU03_15185"
     CDS             2250..>2540
                     /locus_tag="VNU03_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP75864.1"
                     /translation="MTPAGPVLSLRGVTKSFPLADGGALQVLDVEHLELPASGCTVIR
                     GRSGSGKTTLLNLIAGVSAPTGGTIVVHGTEITALPEARRDRFRAERIGYVFQ"
BASE COUNT          408 a          906 c          901 g          325 t
ORIGIN      
        1 gccggcagct cgagcaggag ccgcatgatc agcgccgggc cgatgccgtc gggcgccgga
       61 tcgacgcctc gctccaccag gaagggcgcg gccatcggca gcgcgaggtc gagcggcgtg
      121 gcgaagcgga gattcgccca gccggtctcc gcgagggcca gccgctcgcc cagcgtgtgc
      181 cccagggtat gggtaggcac gatcacccac ttggcgcggg tgcgctcggt gcggcagagc
      241 tcggcgagct gggcgaggaa cgggctcacg cggactccat tgtagatcgt ggatcccggg
      301 gccggcgctc tcagtgacgc aataggggca gcgtcggctg cgcggagcgg cccgacccga
      361 ctacgagttt ttcgggacgc ggcgattccc ccgcaccgag tagaccacga gggcgccggc
      421 caccgcgacc agcccggcga gcgccagcca gcgctccacg cggtgcacgt cggccaggat
      481 ggcgttgatc tggtgcgtga agaagtaggc caggccgaac gcgagcggga cggtcaccgc
      541 cgccgcggcc gcgtcggcca ggatgaactt ccagaacggc acccgcgcga tgcccgcggt
      601 caggaacgcc gccgcgcgca gcccggtcac gtggcgcgcc atcgccaccg tcttcatggc
      661 gtgctcgcgg tatgcggcct tcagccagtc cgcgcgcgcc cgggtcagca cgtggcgcac
      721 caggcgccag cgcagcaggc gctcgcccca gcgccgcccg gcccagtaga gcacgacgtc
      781 gccggccagc accccgagca cgcagaccgg cagcgcgagc caccaccggg cgagcccctc
      841 gtggctcagg atgccggcgg ccacgatcgg catctcctcg ggaatcggca cgcccacgct
      901 cccgagcagc agagcggcca ggatgccgag atacgtgaac cgctgcagga gctcctggag
      961 catggctcgt cagggtagca ccgtcgcgcg ctccgggggg gccttgacga ggtccgcgcc
     1021 ccgagtacta tcggcgcggg tgcccaatgg gaccgggccg tcgtcgcccc gggaacacct
     1081 ccggggcgcg gctcgatcgc ccgaaggcaa acgcgcggtt cacagcgttc acggagggcc
     1141 aagccatgcg tcacctcaca ctgccccggg cggcctcgac gatcctggcg ctcctcttcc
     1201 tcctcgcgcc tgccgtcgcg cgcgcccagc tggcgccgtc gtactcgcag gacgatcagc
     1261 tggccaacga gccggccccc tggacggtcc ccggcaagtg gggcccgcgc ggcaactggg
     1321 ggcgatgggg cgagcaggac aagcgcggca tgctcaactt cctcaccccc gacctgatcg
     1381 tgaaagcggc cggcctggtc aagcagggca aggtgtacgc gctcggcgag gagatgcaca
     1441 gcgatctccc gcgcgcgatc actccgagcc gggccggggt gcagatcatc cacgagaacg
     1501 acggctacga tcgggtgacc cagcccgggc agttcgaccc caagcgcaac cagggcgcgg
     1561 ccagcttcac gatcatgcac aaccacgtgg gcacgcacct cgatacgttc gcgcacatct
     1621 accgcgagaa ttccctgttc aacaacatgc cgccgcccag gccggtgggc accgtgcacg
     1681 gcgacgcggc gagcgtgaag ttcatcgtgg ggcgcggcgt cctcctcgac gtggcgaagt
     1741 acaagggcac cgacccgctg ccggtcaact actggatcac caccgccgac ctgcagaaca
     1801 ccgcgaaggc gcagggggtg gagatccgga agggcgacgt cctgctcatc cgcaccggct
     1861 ggcggaagat gtgggacgag ccgggtccgg atggacgggt ggacctgtcg cacgccaagt
     1921 acctgcagcc ggagccgggc gtcgggcccg acaccctccg cttcctgaac gacatggaga
     1981 tcgtggcgat cggggcggac accgcggcgg tcgagtggaa cttcccgctc caggcgggct
     2041 attcgcggaa ggcgttcggc gggttccagg ggctcccgct gcacgtcgac ttcatctgga
     2101 accgcggcgc ctacatcctc gagatcatga acctggacga gctggccaag gaccaggcct
     2161 acgagttcct cttcgtcgtc gctccgctcc tgctgcgagg cgggatcggg gtgccggtga
     2221 atcccatcgc catccggtag caggaggtca tgaccccggc ggggccggtc ctgagcctgc
     2281 gcggggtgac gaagtcgttc cccctcgcgg acggcggcgc gctccaggtg ctcgacgtcg
     2341 agcacctgga gctgcccgcc tcgggctgca cggtgatcag gggccgcagc ggctcgggca
     2401 agaccacgct gctcaacctg atcgcggggg tgtcggcgcc caccggcggc acgatcgtcg
     2461 tccacggcac cgagatcacc gcgctgcccg aggcgcggcg tgaccggttc cgggcggagc
     2521 ggatcggcta cgtcttccag
//
LOCUS       DATMCV010000662         5572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_437772, whole genome shotgun sequence.
ACCESSION   DATMCV010000662 DATMCV010000000
VERSION     DATMCV010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437772"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VNU03_15190"
     CDS             complement(<1..769)
                     /locus_tag="VNU03_15190"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWP75865.1"
                     /translation="MSPTALPDARAAGAHRAAGRVVDILELVVSARDGLALRELSAQL
                     VAPKSSLLPLLRTLAARGYLEQGPGGEYRLGHRAVELGLGQPAGHGLPEIARPSLRAL
                     MERTGETVFLGTLSGDGAAVVFVDKVDSDQVIRYAGGVGERRPLHATSSGKVILAFLP
                     APQREKILRALPLKRYTDHTVTSLAALRATLDEVRETGLCVNLDELAVGAAGIAAPIF
                     DRDGAVAGACAIGGPTDRVRPRLRALTAEVKASARAIS"
     gene            complement(766..1983)
                     /locus_tag="VNU03_15195"
     CDS             complement(766..1983)
                     /locus_tag="VNU03_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP75866.1"
                     /translation="MTPTPTASTDIVVNEPNRWRLDTPGHAGWPRTARPDDPRKYFMV
                     SADCHANEPHDLWATRIDEQYRARVPKVITDENGVKWRMSEGHRPDRLRTDALEGEDG
                     LRQRVGADPVERLRDMDRDGIDVEVIFPNKGLAMWATPDPVFAMAQCRVFNDWAHETF
                     GPYRDRMAPAAALATGDLEGSIAEVARAAKRGFRLLTLPCKPIWGGHDVDHPNYNLPM
                     FDPLWTVIQDANLPVTFHVSTGRDPRAARGNGGAVINYVSHSLSPTIEPVANLCASGV
                     FERFPRLRVATIEAGIGWVPWFLGAMDEAYRKHHFWVRPKLGRLPSEYYREHCWASFQ
                     EDPAGLALAEPMGLADRFMWGNDYPHHEGTWPHSAEAIERTMGHLSEPTRANMLGLNA
                     ARFFGFEVPAARR"
     gene            complement(1980..2429)
                     /locus_tag="VNU03_15200"
     CDS             complement(1980..2429)
                     /locus_tag="VNU03_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWP75867.1"
                     /translation="MSAAAYLPAGMPRPVAEGDGLDRPYWEGTRAGRLLVQRCRGCRG
                     FQWGPEWICHRCLGADLEWVETAPRGHIYSWERAWHPVHPALKDHGPYIVVLVELPDA
                     GSVRMIGNLLGDPTQEVPIGAPVEAVFEPHDDATPPFTLVQWRRTAP"
     gene            complement(2426..3610)
                     /locus_tag="VNU03_15205"
     CDS             complement(2426..3610)
                     /locus_tag="VNU03_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWP75868.1"
                     /translation="MSASRTIRGRVAIAGIGETAYYKHGRAPESEVALALQAILRACE
                     DAGIDPRQVDGFASYSNDRNDPSRLAAALGLPELRFSNMQWGGGGGGGSAAVGNAAAA
                     VAAGYADCVVVFRALAQGQFQRFGAAPPGGTVSGEAALTFPYGLISPAQRFAMRVMRF
                     MHDHGVRQEAQRAIALASYHHAQANPRAVMHGRPLTPEAYDASRWIVEPFRLFDCCME
                     NDGAAALVLVSAERARDLRHPPAYLLGVAQGSEYRNAARGHNAPTYASSSFTTVAPHL
                     WEMAGLGPKDVHVVQSYENFTGGVLMSLVEHGFFAPEEANDFLTPENLIAPGGRLPLN
                     TSGGNLAECYMHGLELQIEAVRQLRGESTSQVPGARVGLVISGPMVTPVSSMLVGTGD
                     AL"
     gene            complement(3704..4156)
                     /locus_tag="VNU03_15210"
     CDS             complement(3704..4156)
                     /locus_tag="VNU03_15210"
                     /inference="COORDINATES: protein motif:HMM:NF017045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HWP75869.1"
                     /translation="MTGDTVVIAGLPMRDDRAELAGYEADAVDLTAQERRRARRRVVT
                     RAGRVFTLMLPPGEVLAPGRLLYVGPGWYVTVEAAAEALLAVSPRSEREGIRVALEVG
                     RLHAALAVADERLLVPDEPALERLLDRLDVPWTRTHAPFLPITTGSPH"
     gene            complement(4153..4836)
                     /locus_tag="VNU03_15215"
     CDS             complement(4153..4836)
                     /locus_tag="VNU03_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75870.1"
                     /translation="MDQPDDIAGRIVLVVDAALPAAARETCAEAAAALAQRTGVPVTL
                     ATVPIETLDQAQPLLRRSPARIRELAPPTSDAGAMAAAPFVWREGRPDWGAMWTTFCE
                     LALHGGPPQRGADQTLRAPAPGGPAGGSAPEILAELARGILETTGLVAEAGGPGLIAI
                     RCESARMAAWLCAAIILENVEARVEGDRLLLPAGPDFRLEDQVKSVITVVAKTHHYWR
                     EHLETGRRA"
     gene            complement(4957..>5572)
                     /locus_tag="VNU03_15220"
     CDS             complement(4957..>5572)
                     /locus_tag="VNU03_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWP75871.1"
                     /translation="VLGVALRALARVVPMPDPLVVSAFFLRPTLPGPVEVHTEEVRGG
                     RRIATGAARVRQDGKEMVRVTAAFADLAAATGRTVTLAEKPALPPPDEAVDLLSRGAI
                     PGLTLTERVEYRVAALPGWALGRPSGRASMEFWMRYRDGRDADSLSLPALVDAAAPVI
                     LDLGEVGSATIELTVHVRARPAPGWLACRSTTRFVAGGFHEEDF"
BASE COUNT          734 a         2077 c         2064 g          697 t
ORIGIN      
        1 cggagatcgc gcgcgcggac gccttcacct ccgcggtgag agcgcggagc cggggacgca
       61 cgcgatcggt ggggccgccg atggcgcagg cgcccgccac cgcgccgtcg cggtcgaaga
      121 tcggggcggc aatgccggcc gcgcccacag ccagctcgtc gaggttcacg cagagcccgg
      181 tctcgcgcac ctcgtccagc gtggcgcgca gcgcggccag gctggtgacg gtgtggtcgg
      241 tgtagcgctt gagcggcagc gcgcggagga tcttctcgcg ctgcggggcg ggcaggaacg
      301 cgaggatcac cttcccgctc gaggtggcgt ggagcgggcg gcgctccccc accccgcccg
      361 cgtagcggat gacctggtcg ctgtcgacct tgtccacgaa gaccaccgcg gcgccgtctc
      421 cgctgagcgt ccccaggaac accgtctccc cggtgcgctc catcagcgcg cgcagcgacg
      481 gccgggcgat ctcgggcagc ccgtgcccgg ccggctgccc cagcccgagc tcgacggccc
      541 ggtggccgag gcggtactcg cccccgggcc cctgctcgag gtacccgcgg gcggccagag
      601 tgcggagcag cgggagcagg ctgctcttgg gcgcgacgag ctgcgcgctc agctcgcgca
      661 gggcgagacc gtcgcgcgcc gagaccacca gctccaggat gtccaccacc cggcccgcgg
      721 cgcggtgggc tcccgccgcg cgcgcgtccg gcagcgcggt ggggctcacc ggcgcgccgc
      781 cggcacctcg aagccgaaga agcgtgccgc gttcagcccg agcatgttgg cccgcgtcgg
      841 ctcggagaga tggcccatcg tccgctcgat ggcctcggcc gagtgcggcc acgtgccctc
      901 gtggtgggga tagtcgttgc cccacatgaa ccggtcggcg aggcccatcg gctcggccag
      961 cgcgaggccg gccggatcct cctggaacga ggcccagcag tgctcgcggt agtactcgct
     1021 cggcaggcgc ccgagcttcg ggcgcaccca gaagtggtgc ttgcgatacg cctcgtccat
     1081 cgcgcccagg aaccacggca cccagccgat gccggcctcg atcgtcgcca cccgcaggcg
     1141 cgggaagcgc tcgaacacgc cggaggcgca gaggttcgcc accggctcga tggtgggcga
     1201 gagcgagtgc gagacgtagt tgatcaccgc gccgccgttg ccgcgcgcgg cccgcgggtc
     1261 gcgcccggtc gagacgtgga aggtcaccgg caggttggcg tcctggatca ccgtccagag
     1321 cgggtcgaac atcggcagat tgtagttcgg gtgatccacg tcgtggccgc cccagatcgg
     1381 cttgcagggc agcgtcagca gccggaagcc ccgcttcgcc gcgcgcgcca cctccgcgat
     1441 cgagccctcc aggtcgccgg tggccagcgc ggcggcgggc gccatgcggt cgcggtaggg
     1501 cccgaatgtc tcgtgcgccc agtcgttgaa gacgcggcac tgggccatcg cgaagaccgg
     1561 atcgggggtg gcccacatcg cgagcccctt gttggggaag atcacctcga cgtcgatgcc
     1621 gtcgcgatcc atgtcgcgca gacgctcgac cggatcggcg cccacccgct ggcgcaggcc
     1681 gtcctcgccc tcgagcgcgt cggtgcgcag gcgatcgggc cggtggccct cggacatgcg
     1741 ccacttcacc ccgttctcgt cggtgatcac cttcggcacg cgcgcgcggt actgctcgtc
     1801 gatgcgggtg gcccagaggt cgtgaggctc gttggcgtgg cagtccgccg agaccatgaa
     1861 gtacttccgc gggtcgtccg gccgcgcggt gcggggccag cccgcgtggc cgggcgtgtc
     1921 gaggcgccag cggttgggct cgttgacgac gatgtcggtc gaagccgtcg gggtcggggt
     1981 catggggcgg tcctcctcca ctgcacgagc gtgaacgggg gcgtggcgtc gtcgtgaggc
     2041 tcgaagaccg cctccaccgg ggcgccgatc ggaacctcct gggtgggatc tccgagcagg
     2101 ttgccgatca tgcggacgga gcccgcgtcg ggcaattcca ccaggacgac gatgtaggga
     2161 ccgtggtcct tgagcgcggg gtgcaccgga tgccaggcgc gctcccagga atagatgtgg
     2221 ccgcgcgggg ccgtctccac ccactcgaga tcggcgccga ggcagcggtg gcagatccac
     2281 tccggccccc actggaaccc ccggcaccct cggcagcgct ggacgagcag ccgccccgcg
     2341 cgcgtgccct cccagtacgg ccggtcgagc ccgtcgccct cggccaccgg acgcggcatg
     2401 ccggcgggca aatacgccgc cgcgctcaca gggcgtcccc ggtgccgacc agcatgctgg
     2461 agaccggagt caccatcggg cccgagatca cgaggcccac ccgggcgccc ggcacttggc
     2521 tcgtggactc gccgcggagc tggcgcaccg cctcgatctg cagctcgagc ccgtgcatgt
     2581 agcactcggc caggttgccg ccgctggtgt tgagcggcag gcgcccgccg ggcgcgatca
     2641 ggttctcggg ggtgaggaag tcgttggcct cctcgggggc gaagaagccg tgctcgacca
     2701 ggctcatcag cacgccgccg gtgaagttct cgtagctctg gaccacgtgc acgtccttgg
     2761 ggccgaggcc cgccatctcc cagagatggg gcgccaccgt ggtgaagctc gaggaggcgt
     2821 aggtcggcgc gttgtggccg cgcgcggcgt tgcgatactc cgagccctgc gcgacgccca
     2881 gcaggtaggc aggcgggtgg cgcagatcgc gcgcccgctc ggccgagacc agcacgagcg
     2941 cggccgcgcc gtcgttctcc atgcagcagt cgaagaggcg gaagggctcg acgatccacc
     3001 gcgaggcgtc gtaggcctcg ggcgtgagcg gccggccgtg catgaccgct cgcgggttcg
     3061 cctgcgcgtg atggtaggac gcgagcgcga tggcgcgctg cgcctcctgc cgcaccccgt
     3121 ggtcgtgcat gaagcgcatc acccgcatcg cgaagcgctg ggccggcgag atgaggccgt
     3181 aggggaacgt gagcgccgcc tcgcccgaca ccgtgccgcc cggaggcgcg gccccgaagc
     3241 gctggaactg gccctgcgcc agggcgcgga agaccaccac gcagtcggcg taccccgccg
     3301 ccaccgcggc ggccgcgttg cccaccgccg ccgagccgcc gcctccgccc ccgccccact
     3361 gcatgttgga gaagcgcagc tcgggcaggc cgagcgcggc ggcgagccgc gagggatcgt
     3421 tgcgatcgtt gctgtaggag gcgaagccgt ccacctggcg cgggtcgatc ccggcgtcct
     3481 cgcaggcgcg caggatggcc tgcagcgcca gcgcgacctc cgactcgggc gcccgcccgt
     3541 gcttgtagta cgccgtctcg ccgatcccgg cgatcgcgac ccgtccccgg atggtgcgcg
     3601 acgcgctcat ccccgcctcc tcattcttat tctggaatgc cgttccatat ccggcacggg
     3661 gaaggtatcc cagcgcgcgg gcgttgtcac gcgcgatctg gcctcagtgc ggcgagccgg
     3721 tggtgatggg caggaagggg gcgtgcgtgc gcgtccacgg cacgtccagg cgatccagca
     3781 gccgctcgag cgcgggctcg tccggcacga gcaaccgctc gtcggcgacc gcgagcgcgg
     3841 cgtggaggcg gcccacctcg agcgcgacgc ggatgccctc ccgctcgctg cgcgggctca
     3901 cggccaggag cgcctccgcc gccgcctcca ccgtgacgta ccaccccggg ccgacgtaga
     3961 ggagccggcc gggcgcgagc acctcgcccg gcggcagcat cagggtgaag acgcggccgg
     4021 cccgggtgac cacgcggcgc cgggcgcggc ggcgctcctg cgcggtcagg tccaccgcgt
     4081 cggcctcgta ccccgcgagc tcggcccgat cgtcccgcat gggcaggccc gcgatgacga
     4141 cggtgtcgcc ggtcacgccc ggcggccggt ctccaggtgc tcgcgccagt agtggtgcgt
     4201 cttggccacc accgtgatga cgctcttcac ctggtcctcg agccggaagt cgggccccgc
     4261 gggcaggagc agccgatcgc cctcgacgcg cgcctccacg ttctccagga tgatggccgc
     4321 gcagagccac gcggccatac gcgcgctctc gcagcggatc gcgatcagcc cggggccgcc
     4381 cgcctccgcg acgagcccgg tggtctcgag gatgccgcgc gcgagctcgg ccagtatctc
     4441 cggcgcggac ccgcccgccg gaccaccggg cgcgggcgcg cgcagtgtct ggtcggcgcc
     4501 acgctgcggg gggccgccgt gcagcgcgag ctcgcagaac gtggtccaca tcgcgcccca
     4561 gtccggccgg ccctcgcgcc agacgaaggg cgcggcggcc atcgccccgg cgtccgaggt
     4621 cggcggcgcc agctcccgga tgcgggcggg gctccggcgg aggagaggct gggcctggtc
     4681 gagggtctcg atcggcacgg tggcgagggt caccgggacg ccggtgcgct gcgcgagcgc
     4741 cgccgcagcc tccgcgcacg tctcccgcgc ggccgccggg agcgcggcgt ccacgacgag
     4801 cacgatgcgc cccgcgatgt cgtccggttg gtccatttcc gcgcccggat cataccaccc
     4861 gcgctcccgc cggctcccgc gcgcccgggc ggcggatcag ggcagcagcg cgagctggcg
     4921 cgactgggcc acgagcacgc cggccccgtc ccagatctag aaatcctcct cgtggaagcc
     4981 gccggccacg aagcgcgtgg tggagcggca cgcgagccag ccgggggcgg gacgcgcgcg
     5041 cacgtgcacc gtcagctcga tggtggccga cccgacctcg cccaggtcga ggatcaccgg
     5101 ggccgccgcg tccaccagcg cgggcaggct gagcgaatcc gcgtcgcgtc cgtctcggta
     5161 gcgcatccag aactccatgc tcgcccggcc ggagggccgc cccagcgccc agcccggcag
     5221 cgcggccacg cgatactcga cgcgctcggt gagggtgagc ccgggaatcg cgccgcgcga
     5281 taacagatcc acggcctcgt cgggcggcgg cagcgccggc ttctcggcga gggtcacggt
     5341 gcgcccggtc gcggccgcga gatcggcgaa ggccgcggtg acccgcacca tctccttgcc
     5401 gtcctgccgg acgcgcgccg ccccggtggc gatgcgccgc ccgccgcgca cctcctcggt
     5461 gtgcacctcc accggccccg gcagcgtggg ccgcaggaag aaggccgaca ccacaagcgg
     5521 gtccggcatc ggcacgacgc gcgccagcgc ccgcagcgcc acgcccagca cg
//
LOCUS       DATMCV010000663         3279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_302551, whole genome shotgun sequence.
ACCESSION   DATMCV010000663 DATMCV010000000
VERSION     DATMCV010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302551"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1170
                     /locus_tag="VNU03_15225"
     CDS             244..1170
                     /locus_tag="VNU03_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWP75872.1"
                     /translation="MKLLLFDDFRLGVLRGDQVVDVADVVKDVPHTGPGDLMSGLIAR
                     FADYRKKIEEAAGRGKPVPVSGVRIRPPLPKPTNIVCMAVNYMEDGTRSEPAPINAFM
                     KSPGAVIGHGDTMVLPDVPATIFEGEAEVALIIGKRATAVPAARAMEYIFGYCNFIDG
                     SARGLLPTGNTFYQMKSRDTFAPMGPYLVTADEIKDPHQLPIRLSVNGTVKQSFNTSD
                     MAHKIPRCIEWVTSIHTLEPGDVLATGTNHRGLSGFQDGDRIELETEGLGKLQFNIRD
                     ELKRTWSRETRLDRQEKGQDGTTPQLTGKHKK"
     gene            1167..2255
                     /locus_tag="VNU03_15230"
     CDS             1167..2255
                     /locus_tag="VNU03_15230"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWP75873.1"
                     /translation="MKISHVTTRVLSTPADNPLVIGLPAPADTREFVLLELGTDQGLV
                     GIGLTFFGGALTPALRAAVDGLARLTIGMDPSQVEAIAAKCRRLAGSSGPGGIFSLAL
                     SAVDLACWDLMGKAAGRSVCALLGGLRDRAPTYASGALMRQHPVDYLAKAGPRLRDMG
                     FRQMKMQCGSEPTVAASVERVRVMREAIGADVDLMCDINQLWSVHHAVDVGQRIRDHH
                     LFWLEDPTAHDDFAGLARIADALDTPIAAGEYHYGIVPFRHLLEARSIDIVMIDLLRV
                     GGITQWMKVAGMAEAFNLPVVSHLIPEIHVHLIAAIPNGLTVEYMPWTLQLYEETPKI
                     ENGQLLVRDKPGLGLAFDQAAIKRYQVG"
     gene            complement(2262..3155)
                     /locus_tag="VNU03_15235"
     CDS             complement(2262..3155)
                     /locus_tag="VNU03_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015734726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP75874.1"
                     /translation="MERRRLGRTDMDVSVLGFGGSEIGYERAGARTVARLLGGALDAG
                     LNVIDTAECYEDSESLVGAALGSRRDEIHLLTKCGHPRGYGRGDWREASLLSSIERSL
                     KRLRTDRLDLIQLHSCELSDLRKGEAITALERARERGWTRYIGYSGDGEAARFAVECG
                     RFDTLQTSVSIADQEAIERTLPLAMKRGMGVIAKRPIANAAWRYARRPSEPYYQPYWE
                     RLRALEYPFLRDAERVVSTALRFTLAAPGVHTAIVGTKRPERWPENARLLQAGPLPAA
                     EIDQIRARWREIARPSWTGEV"
BASE COUNT          495 a         1196 c         1123 g          465 t
ORIGIN      
        1 cgtgtcagct tgatcaggat cgcgccgggc tccggatccg gcaccggata ctcgcgcagc
       61 tcgaactcgc cgccgtacgc cttcagcacc gccacccgtc ccttcatggg gctcctccct
      121 ggaatggatt cagcgagagg gtcgccgtcg cggagaccgt tgtcaacacc ccgggccgcg
      181 agtaggatgg gcgcggctcc gcggctccag gggacgaagc tccgcgcgac aaaggaggac
      241 accgtgaaac tgctactctt cgacgatttc cggctgggcg tgctccgtgg cgatcaggtg
      301 gtggacgtcg ccgacgtcgt gaaggacgtc ccccacaccg ggcccggcga cctcatgagc
      361 gggctcatcg cccgcttcgc cgactaccgc aagaagatcg aggaggcggc cggccgcggc
      421 aagccggtcc cggtgagcgg ggtgcgcatc cggcccccgc tgcccaagcc caccaacatc
      481 gtgtgcatgg cggtgaacta catggaggac ggcacccgca gcgagccggc cccgatcaac
      541 gccttcatga agtcgcccgg cgcggtgatc ggccacggcg acaccatggt gctgcccgac
      601 gtgcccgcca ccatcttcga gggcgaggcc gaggtggccc tgatcatcgg caagcgcgcg
      661 accgcggtgc cggccgcgcg cgcgatggag tacatcttcg gctactgcaa cttcatcgac
      721 ggctcggccc gagggctcct gcccaccggc aatacgttct accagatgaa gtcgcgcgac
      781 accttcgccc ccatgggccc gtacctggtg accgccgacg agatcaagga tccgcaccag
      841 ctgccgatcc ggctctcggt caacggcacc gtcaagcagt cgttcaacac gagcgacatg
      901 gcccacaaga tcccgcgctg catcgagtgg gtgaccagca tccacacgct cgagccgggc
      961 gacgtgctcg ccaccggcac caaccaccgc gggctctccg gcttccagga cggcgaccgc
     1021 atcgagctcg agacggaagg gctcggcaag ctccagttca acatccggga cgagctcaag
     1081 cgcacgtggt cgcgcgagac gcggctcgac cggcaggaga aggggcagga tggcaccacg
     1141 ccccagctca ccggcaagca caagaaatga agatctccca cgtcaccacc cgcgtgctct
     1201 cgaccccggc ggacaatcca ttggtgattg gtctacccgc tccggccgat acgcgggagt
     1261 tcgtcctcct cgagctgggc accgaccagg ggctggtggg catcgggctc accttcttcg
     1321 gcggcgcgct cacccccgcg ctgcgggccg cggtggacgg cctcgcgcgt ctcaccatcg
     1381 gcatggaccc gagccaggtc gaggccatcg cggcgaagtg ccggcgcctg gcgggcagct
     1441 cggggccggg cggcatcttc tccctcgcgc tgtccgcggt ggacttggcg tgctgggacc
     1501 tcatgggcaa ggcggcgggc cgctcggtgt gcgccctgct cggcggcctc cgcgaccgcg
     1561 cgccgacgta cgcgagcggc gccctgatgc gccagcaccc ggtggactac ctcgccaagg
     1621 ccggcccgcg cctgcgcgac atgggcttcc gccagatgaa gatgcagtgc gggagcgagc
     1681 ccaccgtcgc ggcctcggtg gagcgggtgc gggtgatgcg cgaggccatc ggggccgacg
     1741 tggacctgat gtgcgacatc aaccagctct ggagcgtgca tcacgccgtc gacgtggggc
     1801 agcgcatccg tgaccaccac ctcttctggt tagaggaccc caccgcccac gacgacttcg
     1861 ccggcctggc ccgcatcgct gacgcgctgg ataccccgat cgcggcgggc gagtaccact
     1921 acgggatcgt gccgttccgc cacctgctcg aggcgcgctc gatcgacatc gtgatgatcg
     1981 acctgctccg cgtgggcggc atcacccagt ggatgaaggt ggcgggcatg gccgaggcct
     2041 tcaatctacc cgtggtgagt catctgattc ccgagatcca cgtgcacctc atcgcggcca
     2101 tccccaacgg cctcacggtg gagtacatgc cgtggacgct gcagctttac gaggagacgc
     2161 cgaagatcga gaacggccag ctcctggtgc gggacaagcc cggcctcggc ctggccttcg
     2221 accaggccgc gatcaagcgc taccaggtcg ggtaggcggg gtcagacctc gccggtccac
     2281 gagggcctgg cgatctctcg ccagcgcgcg cggatctggt cgatctccgc ggcggggagc
     2341 ggcccggcct ggagcaggcg cgcattctcc ggccaccgct ccggccgctt ggtgccgacg
     2401 atcgcggtgt gcacccccgg agcggcgagc gtgaagcgca gcgcggtcga caccacacgc
     2461 tccgcgtcgc gcaggaacgg gtactccagc gcgcggagcc gctcccagta gggctggtag
     2521 tagggctcgg acggccggcg cgcgtagcgc cacgccgcgt tcgcgatcgg ccgcttggcg
     2581 atcacgccca tcccccgctt catcgcgagc ggcagcgtcc gctcgatcgc ctcctggtcc
     2641 gcgatgctca ccgaggtctg cagcgtgtcg aagcgcccgc actcgaccgc gaagcgcgcc
     2701 gcctccccgt cgccgctgta gccgatgtag cgcgtccacc cgcgctcccg cgcgcgctcg
     2761 agggcggtga tggcctcccc cttgcggagg tcggacagct cgcagctgtg gagctggatc
     2821 aggtcgagcc ggtcggtgcg caggcgcttg aggctccgct cgatgctgga cagaagggac
     2881 gcctcgcgcc agtcgccgcg gccgtagccg cgcgggtggc cgcacttggt caggaggtgg
     2941 atctcgtccc gccgggatcc cagcgcggcg ccgaccagcg attcgctgtc ctcgtagcac
     3001 tcggcggtgt cgatcacgtt gagcccggcg tcgagcgctc cgccgaggag gcgggccacc
     3061 gtccgcgccc cggcccgctc gtagccgatc tcggagccgc cgaagccgag cacgctcacg
     3121 tccatgtcgg tccgtcccag ccggcggcgc tccatgaccg gacgcgggtc aggccgtccg
     3181 ggacgcggcc gcctcgtcgc gctccatccg cgcccgcatc cgcgcgcgca ggccctccac
     3241 gatgtgcgcg ggggcggtcg cgggggagcc ggcccggaa
//
LOCUS       DATMCV010000664         3180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_32740, whole genome shotgun sequence.
ACCESSION   DATMCV010000664 DATMCV010000000
VERSION     DATMCV010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32740"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VNU03_15240"
     CDS             <1..1357
                     /locus_tag="VNU03_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP75875.1"
                     /translation="FAPSLVQALAFIYVFGNNGILTRTTGLNVGIYGAKGIVLAEVFY
                     CFPHAVLILVAALSATDARLYDAARTLGAGPLKTFLTVTLPGVKFGLVSACFVVFTLV
                     ITDFGAPKAIGGKFSVMATEIYNQVSGQQNFTMGATVSVVLLIPAVLAFLVDRVVQRR
                     RYALVTSSSQPLTPRRRPAADWGTFAYCALVAGAIAGMYLAILVYSLVARWPYNFTPT
                     LRHYRFDTVGGYAPLWNSVYVAALTAVVGTVLTFVGAYVVEKCRATGSGLLYLLSVLP
                     VSIPGMVLGLAYIFTFNAAGSALNALYGTLAILIVSNVIHYFTVGFLTATTALRQMDG
                     EFENVSASLGVPFYRTFWRVTVPMALPSIVAISMYFFLNAMVTLSAVVFLVAPGTELA
                     AVAVLLMDDAGDAAQAAAMSVCIVGIGLAVRLGYWALMRGVDRRTQAWRESREAPAGG
                     P"
     gene            1354..1665
                     /locus_tag="VNU03_15245"
     CDS             1354..1665
                     /locus_tag="VNU03_15245"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP75876.1"
                     /translation="MTQSVIDVPRHRGQFFEILQETAQSQTAVMTIDPGQDGGPAETH
                     AGDQVVYVIEGEGVLTIEGVEHRVRPGVCALIPAGTLHHVKNAGAGPLFFLSVYAPPA
                     Y"
     gene            1668..2405
                     /locus_tag="VNU03_15250"
     CDS             1668..2405
                     /locus_tag="VNU03_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75877.1"
                     /translation="MAPPEKTALVTGAARGIGLAIATRLAADGVRVALLDQDGAAVEA
                     AAGAIGPGALALTADVTRARDVDDAVARVVQRWSRLDILVNNAGITGRSFPIWELTDE
                     DWQRVIDVDLTSVFLCCRAAVKVMLRQGSGRIVNIASIAGKEGNPTLVPYSTAKAGVI
                     GLTKALAKEVATRGILVNAVAPAVIGTELLKQMEPSTVELLVSKIPMGRVGTPEEVAA
                     LVAWLASDECTFSTGAVYDLSGGRATY"
     gene            complement(2402..2959)
                     /locus_tag="VNU03_15255"
     CDS             complement(2402..2959)
                     /locus_tag="VNU03_15255"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWP75878.1"
                     /translation="MPPDPGDREWRFIRSHERARMGKLTLREDVWRLPTGVEKTYPIL
                     HVGLTVGVVPFVDRERVLLVRQFRHLARADSWELPGGGALPGESPEAAAQRELREEGG
                     YRAGRLTFLTRFLPSAAYLDETAHCFIGEELVADPLASDDDEFFERRAVPFREALAMA
                     LDDRITESVSKLALLAAALRLGVNP"
BASE COUNT          429 a         1113 c         1142 g          496 t
ORIGIN      
        1 cttcgcgccc tccctggtgc aggcgctcgc cttcatctac gtcttcggca acaacggcat
       61 cctcacccgg accaccgggc tcaacgtggg catctacggc gcgaagggca tcgtgctcgc
      121 cgaggtgttc tactgcttcc cccacgcggt gctcatcctg gtggccgcgc tctccgccac
      181 cgacgcgcgc ctctacgacg cggcccgcac cctcggggcc ggtcccctca agaccttcct
      241 gaccgtgacc ctgccggggg tgaagttcgg gctggtgagc gcgtgcttcg tggtgttcac
      301 gctggtgatc accgacttcg gggcgcccaa ggccatcggc ggcaagttct cggtgatggc
      361 gaccgagatc tacaaccagg tctcgggcca gcagaacttc acgatggggg cgacggtctc
      421 ggtggtgctg ctgatcccgg cggtgctcgc cttcctggtc gaccgggtgg tgcagcgccg
      481 ccgctacgcg ctcgtcacct cctcctcgca gccgctcacg ccccgccggc ggcccgccgc
      541 ggactggggc accttcgcct actgcgcgct ggtggcgggg gccatcgcgg ggatgtacct
      601 cgcgatcctc gtctactccc tggtggcgcg ctggccgtac aacttcaccc cgaccctgcg
      661 ccactaccgc ttcgacaccg tgggcgggta cgcgcccctc tggaacagcg tctacgtggc
      721 cgcgctcacc gcggtggtcg gcaccgtgct caccttcgtg ggggcctacg tggtcgagaa
      781 gtgccgggcg accgggagcg ggctgctcta cctgctctcg gtgctgccgg tgtccatccc
      841 ggggatggtg ctgggcctgg cctacatctt cacgttcaac gcggcgggaa gcgcgctgaa
      901 cgcgctctac ggcacgctcg cgatcctgat cgtctccaac gtgatccact acttcacggt
      961 gggcttcctc accgccacca ccgcgctccg gcagatggac ggcgagttcg agaacgtgtc
     1021 ggcctccctc ggggtgccgt tctaccgcac cttctggcgg gtcacggtgc cgatggcgct
     1081 gccgtcgatc gtggccatca gcatgtactt cttcctcaac gcgatggtga ccctctcggc
     1141 ggtggtcttc ctggtggccc ccggcaccga gctggccgcg gtcgcggtgc tcctcatgga
     1201 cgatgcgggc gacgccgcgc aggcggccgc gatgtcggtc tgcatcgtcg gcatcggcct
     1261 cgccgtccgc ctcggctact gggcgctcat gcgcggcgtc gaccgtcgca cgcaggcctg
     1321 gcgcgaatcc cgggaagccc ccgccggagg cccatgaccc aatcggtgat cgacgtcccg
     1381 cggcatcgcg ggcagttctt cgagatcctg caggagaccg cgcagagcca gaccgcggtc
     1441 atgaccatcg atcccgggca ggacgggggg ccggcggaga ctcacgccgg cgaccaggtc
     1501 gtgtacgtga tcgaggggga gggcgtcctg accatcgagg gcgtcgagca tcgggtgagg
     1561 ccgggggtct gcgcgctgat cccggccggg acgctccacc acgtgaagaa cgcgggggcg
     1621 gggccgctct tcttcctctc ggtgtacgcc ccgccggcct actagcgatg gcgcctccgg
     1681 agaagaccgc gctcgtgacc ggcgctgccc ggggcatcgg gctcgcgatc gccacccggc
     1741 tggccgccga cggcgtgcgg gtggccctgc tcgatcagga cggcgccgcg gtggaggcgg
     1801 cggcgggggc gatcggcccg ggcgcgctcg ccctgaccgc ggatgtcacc cgcgcccgcg
     1861 acgtggacga cgcggtggcc cgcgtggtgc agcgatggag ccgactggac atcctggtca
     1921 acaacgcggg gatcaccggg cgctccttcc cgatctggga gctgaccgac gaggactggc
     1981 agcgcgtgat cgacgtggat ctcaccagcg ttttcctctg ctgccgggcc gcggtcaagg
     2041 tcatgctgcg gcaggggagc ggccgcattg tcaacatcgc ctcgatcgcg ggcaaggagg
     2101 gcaacccgac gctcgtgccc tactcgaccg cgaaggccgg cgtcatcggg ctcaccaagg
     2161 cgctcgccaa ggaggtcgcg acccgcggca tcctcgtcaa cgcggtggcc cccgcggtga
     2221 tcggcaccga gctgctcaag cagatggagc cgagcacggt ggagctgctg gtgtcgaaga
     2281 tcccgatggg gcgcgtgggg acgccggagg aggtcgcggc cctggtggcg tggctcgcct
     2341 ccgacgagtg cacgttcagc accggcgcgg tctacgatct ctcaggcggc cgcgcgacct
     2401 actaagggtt gacgccgagg cggagggcgg cggcgagcag ggcgagcttg gagaccgatt
     2461 cggtgatgcg gtcgtcgagc gccatcgcga gggcctcgcg gaacggcacc gcgcggcgct
     2521 cgaagaactc gtcgtcgtcg gaggcgagcg ggtcggccac cagctcttct cctatgaagc
     2581 agtgcgcggt ctcgtcgaga taggccgccg acggcaggaa gcgggtcagg aaggtgaggc
     2641 gtcccgcgcg atagccgccc tcctcgcgta gctcgcgctg ggcggccgcc tccgggctct
     2701 cgccgggcag cgccccgccg ccgggcagct cccaggagtc ggcgcgagcc aggtggcgga
     2761 attgccggac cagcagcacg cgctcgcggt ccacgaacgg cacgactccc acggtcagtc
     2821 ccacgtgcag gatcgggtag gtcttctcga cgccggtggg cagccgccag acgtcctcgc
     2881 gcagcgtgag cttgcccatg cgcgcccgct cgtggctgcg gatgaagcgc cactcgcggt
     2941 cgccggggtc ggggggcacg ggaagtctcg ccgcgtcagt gtgctatcgt aaggcgcatg
     3001 acgcaagagc ggcccgcgga tcggctgatg gacaagctgc tccgcgccat cgaggtgtcg
     3061 atgaaggcca ccgaggcctc ccgcgaagcg gtgcaggaga tcctccggcg tggggccgag
     3121 accggcatct tcttcgcggg cggccgcgcc ggggacgccg gtgagggctc tcccgaaggc
//
LOCUS       DATMCV010000665         4936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_502646, whole genome shotgun sequence.
ACCESSION   DATMCV010000665 DATMCV010000000
VERSION     DATMCV010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502646"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VNU03_15260"
     CDS             complement(<1..484)
                     /locus_tag="VNU03_15260"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWP75879.1"
                     /translation="MHTPRAPRRRSHTSARPPRCGRKGRPGRYLIVNADDLGADDGTT
                     RGIIAAHERGIVTSASLMVDMPGARSAVRAARAHPQLGLGLHVSFTAGRRIPDLGDVG
                     ALRRELERQLDLFVDLTGQDPTHIDSHHHVHRRAELSPLFVELCRNRRIPLRGFSGIT
                     Y"
     gene            complement(681..2051)
                     /locus_tag="VNU03_15265"
     CDS             complement(681..2051)
                     /locus_tag="VNU03_15265"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP75880.1"
                     /translation="MSDAGGISMDETSVPVSKAARSVEQQRWLDRALAQVDDAGLLRL
                     AMRMVDIPSPTGGEAPLARHLEEDLRGRGFATTYQEIEEHRGNCIARVPGDGSGPDLM
                     LYGHLDTTFTGDAREDYPVVGPADRPDLRPRATLHDGLITGLGISNPKGGAACAIAAA
                     DAVRRAGVPLRGDLVVALVVGGIHKRPTEGVHRDFRGRAYQGFGIGCEYMLKHGVRAD
                     VAISTKPGYTVVWEEPGQCWFTVRVKGLLCYSGLRHIQPSRNPIVDAARLVEALEAWF
                     PEYTRRHTRGQLAPQAAVGAIEGGWPFKPEFIPGICHVYVDLRTNPHQTMLSVKREFD
                     DFMGAFRRAHPEMDLDWEMTLSVPGSRTDPRSWIIQTCLRAWEAVEERPHAFVHSQSG
                     TTDGNVLRNAGIPTARFGLPGLMNPEAGWPPSYDACRVESLGRLARAYVHAIIDTCTR
                     RRDEIG"
     gene            complement(2048..3274)
                     /locus_tag="VNU03_15270"
     CDS             complement(2048..3274)
                     /locus_tag="VNU03_15270"
                     /inference="COORDINATES: protein motif:HMM:NF023151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP75881.1"
                     /translation="MNPTEMRSLIPALGLSEYWYPALSESSVPRDKPTGLKIMGEEIA
                     FFRDKDGSVAAIADVCPHRGGSLRRGDCHYPGTIACPYHGWVFDARGECVAVLSEGPD
                     SRIPGKVRSRIFPTRTVKGMVFVWMGRGAPAPIAEDVPPEFFEGDETIVMTATRYWPV
                     NWRVALENALDSHVMYVHRNALLQLMEPILQFGPSGYRPRIVNGRAALGYIPDDEAPR
                     PAREYYPGVDGHWPKTQWRRLWLWAFRWRLSRRVTRRPFNPDEEWGMHTFLDGKRVRS
                     GGHHLPSMFRFDFGTHMYTRACVPVDEQLTRVIYYHAVRRRSRLGRAAARLFFLTFQN
                     WAMNANFSDQDYRVMAPQRYDTPEKLSGTDAEVIAWRRLLLSARGMPARSDLGPIEDA
                     GDESLADLRTRRSRPR"
     gene            complement(3299..4006)
                     /locus_tag="VNU03_15275"
     CDS             complement(3299..4006)
                     /locus_tag="VNU03_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008858532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75882.1"
                     /translation="MRWLEVDDVSVAYGLVRALDGVSLSVSEGEIVALVGSNCAGKST
                     LMKAVMGVVRPASGRIIFAGRSLLGLAVEAVVRRGVVLVPEGRGILGSLTVRENLRLG
                     AYHRRDAGIKADLDQVTARFPVLASRLEQKAGSLSGGEQQMLAIGRALMARPRLLLLD
                     EPSLGLAPVIVASIFDIIASLAREGVTVLLVEQNAAEALRLAHRAYVLETGRVVLEGK
                     ASDLLQHERVREAYLGA"
     gene            complement(4022..4798)
                     /locus_tag="VNU03_15280"
     CDS             complement(4022..4798)
                     /locus_tag="VNU03_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75883.1"
                     /translation="MRATPTVLRVDRLAKRFGGYQALAGVDLAVEPGSVHAVIGPNGA
                     GKTTLFNVITGILPPTEGDVALEGRDITGWRADRVTRAGIARTFQQVRLFRGMSALEN
                     VMVGRHARTRGGFGAALIRPPFRERGEEAETRRRAQELLELVGLAARAQTSGGDLTLV
                     EQRRLEVARALASDPRLLLLDEPAGGMTPVEVEDLNQLIRKIRESGVTVLLVEHHMRL
                     VMRVSDVVTVLDAGRVIASGSPDTVRRDPRVIAAYLGRDA"
BASE COUNT          690 a         1776 c         1799 g          671 t
ORIGIN      
        1 cgtaggtgat cccggagaat ccgcgcagag ggatgcgacg attccggcac agctcgacga
       61 acagcggcga gagctcggcc cttcgatgga cgtggtgatg cgagtcgatg tgcgtcggat
      121 cctgcccggt caggtcgacg aacaaatcga gctgccgctc gagctctcgc cgcagcgctc
      181 caacgtcgcc gagatcgggg atccgacggc ccgccgtgaa gctcacgtgg aggccgaggc
      241 ccagctgcgg atgcgcccgg gccgcccgga ccgcggagcg agcgcccggc atgtcgacca
      301 tgaggctcgc gctggtcacg atgccccgct cgtgggccgc gatgatcccc cgcgtggtcc
      361 cgtcgtccgc gcccagatcg tcggcgttga cgatcaggta gcggccgggc cggcccttgc
      421 ggccgcatcg tggcggccgg gcactggtgt ggctcctgcg acgcggggct cggggggtat
      481 gcaacggtgg ctccgcccgg agttccgggc aagccagatg ccaccgtcgg atgcgggctc
      541 aaagtagcga cattcaaacg ctcccggtcc cccggcggag ccgtggccct gatcgatcgt
      601 gaacagtgtc aggcgcggtg tgttcgggct ccagacacac cggccggccc gcaggtggga
      661 gccgggagcg cgacaaccga tcacccgatc tcgtcgcggc ggcgcgtgca cgtgtcgatg
      721 atcgcgtgca cgtacgcgcg ggcgaggcga ccgagcgatt ccacgcggca ggcgtcgtag
      781 ctcggcggcc agcccgcttc cgggttcatg aggccgggca ggccgaagcg cgcggtgggg
      841 atgcccgcgt tgcgcagcac gttgccatcc gtggttccgc tctgcgaatg cacgaaggcg
      901 tgcggccgct cctccaccgc ctcccaggcg cgcaggcagg tctggatgat ccagctccgc
      961 gggtccgtcc gggagccggg caccgacagg gtcatctccc agtcgaggtc catctcgggg
     1021 tgggcgcgac ggaacgcgcc catgaagtcg tcgaactcgc gcttgaccga cagcatggtc
     1081 tggtgcgggt tggtgcgcag gtcgacgtag acgtggcaga tgccggggat gaactcgggc
     1141 ttgaagggcc agccgccctc gatggccccg accgcggcct gcggggcgag ctgcccgcgc
     1201 gtgtgccggc gggtgtactc cgggaaccac gcctccagcg cctcgaccag gcgcgcggcg
     1261 tcgacgatgg ggttccggct cggctggatg tggcgcaggc cggagtagca gagcaggccc
     1321 ttcacgcgca ccgtgaacca gcactggccg ggctcctccc acaccacggt gtagccgggc
     1381 ttggtgctga tggcgacgtc cgcccgcacg ccgtgcttca gcatgtactc gcagccgatg
     1441 ccgaagccct ggtaggcgcg gccgcggaag tcgcggtgga cgccctcggt cggccgctta
     1501 tggatgccgc ccaccacgag ggcgacgacg aggtcgcccc ggagcggcac gcccgcccgg
     1561 cgcaccgcat ccgcggccgc gatggcgcag gcggcgccgc ccttcgggtt cgagatgccg
     1621 agcccggtga tgagcccgtc gtggagcgtc gcgcgcggcc ggaggtcggg ccggtccgcg
     1681 ggccccacca ccgggtagtc ctcgcgcgcg tccccggtga acgtggtgtc gagatggccg
     1741 tagagcatca ggtccggccc gctgccgtcg cccgggaccc gggcgatgca gttgccgcga
     1801 tgctcttcga tctcctggta ggtcgtggcg aagccgcggc cgcgcaggtc ctcctcgagg
     1861 tgccgggcga ggggggcctc gccgccggtc gggctcggga tgtccaccat gcgcatcgcg
     1921 aggcggagca gccccgcgtc gtcgacctgg gccagcgcgc gatcgagcca ccgctgctgc
     1981 tcgacggatc gcgccgcctt cgagacggga acgctcgtct cgtccatcga gatcccgccc
     2041 gcgtcgctca tcgcggccgc gaccgccggg tccggagatc ggccagggac tcgtcgcccg
     2101 cgtcctcgat cggccccagg tcgctgcgcg cgggcatgcc gcgcgcgctc agcaggagcc
     2161 ggcgccacgc gatcacctcc gcgtccgtcc cggagagctt ctccggcgtg tcgtaccgct
     2221 gcggcgccat cacccggtag tcctggtccg agaagttggc gttcatggcc cagttctgga
     2281 aggtcaggaa gaacaggcgg gccgcggccc ggcccaggcg cgagcgccgc cgcaccgcgt
     2341 ggtagtagat gacgcgcgtg agctgctcgt cgaccggcac gcaggcccgc gtgtacatgt
     2401 gagtgccgaa gtcgaagcgg aacatgctgg gcaggtggtg gccgccggag cgcacccgct
     2461 tgccgtcgag gaacgtgtgc atcccccact cctcgtcggg gttgaagggc cggcgcgtga
     2521 cgcgccggct caggcgccag cggaaggccc agagccagag gcgccgccac tgcgtcttcg
     2581 gccagtgccc gtccacgccc gggtagtact cgcgggccgg gcgcggggcc tcgtcgtccg
     2641 ggatgtagcc gagggcggcg cgcccgttca cgatccgcgg ccggtagccg gaggggccga
     2701 actggaggat cggctccatg agctggagca gggcgttgcg gtgcacgtac atcacgtgtg
     2761 agtcgagcgc gttctcgagc gccacccgcc agttcaccgg ccagtagcgc gtggcggtca
     2821 tgacgatcgt ctcgtcgccc tcgaagaact cgggcggcac gtcctccgcg atcggcgcgg
     2881 gcgccccgcg gcccatccac acgaagacca tgcccttgac cgtcctggtc gggaagatac
     2941 ggctccggac cttgccgggg atccggctgt ccggcccctc ggagagcacc gcgacgcact
     3001 cgccccgcgc gtcgaagacc cagccgtggt agggacacgc gatggtcccg gggtagtggc
     3061 agtcgccgcg ccggagcgag ccgccgcggt ggggacacac gtcggcgatc gcggccacgg
     3121 atccgtcctt gtcccggaag aaggcgatct cctcgcccat gatcttgagg ccggtgggct
     3181 tgtcgcgagg cacggacgat tcggacaggg ccgggtacca gtactcggac aggcccagcg
     3241 cggggatcag cgatcgcatc tcggtcgggt tcatgtcgtc attcctccgt gggccgcgtc
     3301 acgcgcccag ataggcctcg cgcacgcgct cgtgctgcag caggtccgac gcttttcctt
     3361 ccagcaccac gcgccccgtc tcgagcacgt aggcccggtg ggcgaggcgg agcgcctcgg
     3421 ccgcgttctg ctcgacgagg aggacggtga cgccctcccg ggcgagcgac gcgatgatgt
     3481 cgaagatgct cgccacgatg accggcgcca ggccgagcga gggctcgtcg agcagcagca
     3541 ggcgcgggcg ggccatcagg gcgcggccga tggccagcat ctgctgctcg ccgccggaga
     3601 gcgagcccgc cttttgctcg aggcggctcg ccagcaccgg gaagcgcgcg gtgacctggt
     3661 cgaggtcggc cttgatgccc gcgtcgcgcc ggtgataggc gccgaggcgc aggttctcgc
     3721 gcacggtcag ggagcccagg atcccgcggc cctcgggcac cagcaccaca ccccggcgga
     3781 ccaccgcctc gacggccagc cccagcagcg agcggcccgc gaagatgatg cggccgctcg
     3841 ccggccgcac cacgcccatc accgccttca tgagcgtgct cttccccgcg cagttcgatc
     3901 cgacgagggc cacgatctcg ccctccgaga cggagaggct caccccgtcg agcgcgcgga
     3961 ccaggccgta ggccaccgac acgtcgtcga cctcgagcca tctcatgcgt cgcgtccgcc
     4021 tttatgcatc tcggccaaga taggcggcga tcacgcgggg atcccgccgc accgtgtccg
     4081 gcgatccgct cgcgatgacc cggcccgcgt cgagcacggt gaccacgtcg gagacgcgca
     4141 tcaccaggcg catgtggtgc tcgaccagga gcacggtcac tccgctctcg cggatcttgc
     4201 ggatgagctg gttcaggtcc tccacctcga ccggggtcat gccgccggcc ggctcgtcga
     4261 ggagcaggag gcgcgggtcg ctcgcgaggg cgcgcgccac ctcgaggcgg cgctgctcga
     4321 ccagcgtcag atcgccgccc gatgtctgag cgcgcgcggc gagccccacc agctcgagca
     4381 gctcctgcgc ccggcgccgc gtctccgcct cctcgccgcg ctcgcggaac ggcgggcgga
     4441 tcagcgccgc gccgaagccg ccccgggtgc gggcgtggcg ccccaccatc acgttctcga
     4501 gcgcgctcat cccccggaag aggcgcacct gctggaacgt gcgcgcgatg ccggcgcggg
     4561 tcacccggtc ggcgcgccag cccgtgatgt cccggccctc gagcgccacg tccccctcgg
     4621 tcgggggcaa gatgccggtg atgacgttga agagcgtggt cttgccggcc ccgttcggcc
     4681 cgatcaccgc gtggacgctc cccggctcca cggccaggtc cacgccggcc agggcctggt
     4741 agccgccgaa gcgcttggcg agccggtcga cgcgcagcac ggtcggggtc gccctcacac
     4801 cgccgggcgc cggcgtcccg aaagcaggcc ggcgggccgg aggaccgcgc agaggaccag
     4861 gagggagccg aacacggtca tccgccagtc ctgcacgaag cgcagcagct cgggcaggag
     4921 cgtgaccacc gccgcg
//
LOCUS       DATMCV010000666         7528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_634982, whole genome shotgun sequence.
ACCESSION   DATMCV010000666 DATMCV010000000
VERSION     DATMCV010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634982"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VNU03_15285"
     CDS             <1..1033
                     /locus_tag="VNU03_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75884.1"
                     /translation="GDAASLATAPLSLLTLSEPLATRLSRWGLRTLGELVALPTAGLF
                     ERAGAEGVQAQRLARGEDPRPLASWRPVPAFEDSVECEWGIETLEPVVERLSGLAAQL
                     CGRLASRGLAADGFEWVCRLAGGRAHEGALTPAVPLTDAAAVTGLLRLALEARPPRGI
                     VEGLTLRARPTRAGAVQESLTDPSRPSPRFLAATMNRLVALVGSDRIGAPALLDSHRP
                     DAVGLGAYASPSPLRGEGRVRGSEVRGPERGPEVNGSAPAPVLALRRLRPPAPAAVTL
                     ASGRPVAMRSSRLAGRIVAGVGPWRSSGEWWTTQPWLQDEWDVELADGTLCRLAHDGS
                     AWHIAGIYD"
     gene            1060..1938
                     /locus_tag="VNU03_15290"
     CDS             1060..1938
                     /locus_tag="VNU03_15290"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP75885.1"
                     /translation="MTVLVTDMDNTLFDWLEMWQAAFGAMLERLTADSGVPRETLEQE
                     FFAIHQRHGTTEYAFAIQGLPSLRARHPPEELPTRYAAAIEAYRSMRRRTLTLYPGVL
                     DTLRTIRAAGTLVVAYTESRAYYADYRVRMLGLDGGIDYLYSLPDHALPEGLTPSQIR
                     RYPPEHYRLRETVHRHTREGAWKPDASVLLEILREVGAAPTQAVYVGDSLVKDVAMAQ
                     AASVMDVFARYGDVRSRPGYALLRRVTHWSAAMMARSEGIREADVRPTHVLSGGFAEL
                     LGRIDFCRFTPRSSAV"
     gene            1942..5490
                     /gene="dnaE"
                     /locus_tag="VNU03_15295"
     CDS             1942..5490
                     /gene="dnaE"
                     /locus_tag="VNU03_15295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWP75886.1"
                     /translation="MFVELHARSAFSFLEAAALPEALAERAAALGQPSVALLDADGVY
                     GAPRLYRACTRLGLSARVGAEVTLADGSRLPLLVEDREGYQNLCRLLTRVKMRAAKGE
                     GAATLDELSEFAGGLVCLTGGGRGPVATVLEGEGAPAGRRLLERLVSIYGRFNVFAEL
                     QRHLSRPEEARNEWLRAEARRLRLGPLATNEPRLIARRDRPLLDALTCIREHVTLEAA
                     GRRLARNSEHFMKSGATMERLFSDCPEAVANTGELAMRLGFTLKDLGYRFPDYPLPRG
                     ETPIGFLRELAYAGARTRYGSGPLAEKARRQIEHELALIGRLDLAGYFLIVWDLVQYC
                     GAHDVLVQGRGSAANSAVCYALGLTAVDPVGMELLFERFLSEERGEWPDIDLDLPSGD
                     RRERVIQYVYERYGRLGAAMTANVISYRGRSAAREVGKVLDLPADVIERLSQLVSNWE
                     YNDPGDSLLAHLGEAGCDPAEPRGQHFVRLWSAIQDLPRHLGQHSGGMVIAQGRLDGV
                     VPLEPATMPGRSVVQWDKDDCAALGIVKVDLLGLGMMSLLQDALEMIRRRGGAVDLAH
                     LPQDPTVYRMLQRADTIGVFQVESRAQMATLPRLRPTCFYDLVVQVAIIRPGPIVGDM
                     VHPYLRRRAGREEITVPHPDLEPILRRTLGVPLFQEQLLRMAMATAGFTGGEAEELRR
                     AFGFKRSERAMKEVELKLRAGMARQGISGAAAEAIIHSITAFALYGFPECVVGDTRVV
                     DPETGRRVRIEDVVEGRASLSHTLACDPDLKIRTRRVLAATASGSRMVFRLRTFLGRE
                     LVATPEHLLLTEDGWRPLGSLQFDDHVATAEADASCVGWDCVMSVEPAGMSPTYDLAV
                     EEDHNFVADDLVVHNSHAASFALLAYASAYLKAHHPAAFYAALLNNQPMGFYDPATIV
                     NDAGRHGQVIHPADVNYSGWLCTIESMGEPTGSPAGEVRLGLRYVRGLREEAGLRLEA
                     ERKRRVFASVEDLVKRGGLRHDEVAQLARVGALASLEPERRAALWEAERVGRPAGALY
                     EELAAPPETSPLRPMTAEERLVADYDGTGLTLGPHPMAFRRGRLLRMGVSRAADLAAF
                     RHGAWVKVAGAVVVRQRPGTAKGFVFLNLEDETGLINVIVRPALFHRHRLALVSESFL
                     LIEGTLQRQDDVTSVRAERLAPLRHRLANVPSHDFH"
     gene            complement(5414..6367)
                     /locus_tag="VNU03_15300"
     CDS             complement(5414..6367)
                     /locus_tag="VNU03_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWP75887.1"
                     /translation="MDLNPAHVRTLREIARHGSFSRAAESLHLSQPAVSLHMRQLEAR
                     CGTALLERVGKRAFPTRAGTVLLEHAGRAFAELDRAQEALHHLRGVVAGRLRLGTGAT
                     ASIHLLPPLLRRLRTRYPELELIVVTGNAPEIAAAVAANELDVGLVTLPVTGRQLTVT
                     PFRQDPLVAVGPPGHRWPGPGALTAAELAQQPLILYERGGTIRRVIDGWFRRGGARPR
                     VVMELGNEEAIKKLVGAGLGVSVSPEMAVRDEVRAGALRMRPLSPALVRRLGLVRRRD
                     KPETPALRVFLAAVAGLSENRAKARSPGDGAGGRAAPRARR"
     gene            6475..6969
                     /locus_tag="VNU03_15305"
     CDS             6475..6969
                     /locus_tag="VNU03_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HWP75888.1"
                     /translation="MGDVSIRPATLDDAAAICTIYNQGIEDRIATLETELRTPEERRQ
                     WMGGRAARHPVVVAATGGQVVGWGSLNPFNPRPAYDNVVDLSVYVERGWRGRGVGRAL
                     LQHLLPLARTLGYHKMVLATFPYNEAGVALYRKMGFSPVGVYHEQGRLDGRWVDVLIM
                     ERLL"
     gene            7029..>7528
                     /locus_tag="VNU03_15310"
     CDS             7029..>7528
                     /locus_tag="VNU03_15310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75889.1"
                     /translation="MYYGFPVFLASMEADLGASRIAITGAFTVGMAVAALAALPVGRW
                     LDRRGPWGLMTVGSCLGAALMVAWSQVHGVVGLYVIWSLMGLALAATLYEPAFGAVVR
                     WFPTDHRDRALTIVTLAGALASTIFMPISAGFLQHWGWRPALVGLAVILAILTIPLHA
                     VVLRRQP"
BASE COUNT          935 a         2765 c         2757 g         1071 t
ORIGIN      
        1 aggcgacgcc gcctcgctgg ccacggcgcc gctttcgctc ctcacgctct ccgagcccct
       61 ggcgacccgg ctctcgcgct gggggctgcg cacgctcgga gagctcgtgg cgctgcccac
      121 cgcggggctg ttcgagcggg ccggcgccga gggggtccag gcccagcggc tggcccgcgg
      181 ggaggacccc cgcccgctcg cgtcctggcg gccggtgccg gccttcgagg actcggtgga
      241 gtgcgagtgg gggatcgaga ccctcgagcc ggtggtggag cgcttgagcg ggctggccgc
      301 gcagctctgc gggcggctcg cctcgcgtgg cctcgcggcg gacggcttcg agtgggtctg
      361 ccggctcgcg ggtgggcgcg ctcacgaagg cgcgctcacc cccgccgtgc cgctgaccga
      421 tgccgccgcg gtgacaggcc tgctgcggct cgccctcgag gcccggcccc cgcgcggcat
      481 cgtcgagggt ctcaccctgc gagcccggcc cacccgggcg ggggcggtcc aggagtcgct
      541 caccgatcct tcacgtccga gcccacggtt cctcgcggcc accatgaacc gtctggtcgc
      601 gctggtgggc tcggaccgca taggcgctcc ggcgctgctc gacagccatc gccccgacgc
      661 cgtgggcctg ggcgcctatg cctctccctc tcccctcagg ggagagggca gggtgagggg
      721 ttctgaggtg aggggccccg agaggggccc cgaggtgaat ggctccgcgc ccgctcccgt
      781 cctcgccctc cgccgtctcc gccccccggc gcccgccgcg gtgaccctcg cctccggccg
      841 cccggtggcg atgcgctcga gccgactcgc ggggcgcatc gtcgccggcg tggggccgtg
      901 gcgctcgtcc ggcgagtggt ggaccacgca gccgtggctc caggacgagt gggacgtgga
      961 gctggccgac ggcacgctct gccgcctggc ccacgacggc tcggcctggc acatcgccgg
     1021 catctacgac tgatgggcgc cgcgcccgga cgtcccgccg tgacggtcct ggtcacggac
     1081 atggacaaca ccctcttcga ctggctcgag atgtggcagg ccgcgttcgg cgcgatgctc
     1141 gagcggctca ccgccgacag cggcgttccg cgcgagacgc tcgagcagga attcttcgcc
     1201 atccaccagc gccacggcac caccgagtac gccttcgcga tccaggggct gccgtccctg
     1261 cgcgcccgcc acccgccgga agagctgccg acccggtacg cggccgccat cgaggcctac
     1321 cgctcgatgc ggcgccggac cctcacgctc taccccgggg tcctcgacac cctgcggacg
     1381 atccgggccg cgggaacgct cgtcgtggcc tacaccgagt cccgcgccta ctacgccgac
     1441 taccgggtgc gtatgctcgg cctcgacggc gggatcgatt atctctactc gctgcccgac
     1501 cacgctctcc ccgagggcct gacgccgtcg cagatccggc gctacccgcc cgagcactac
     1561 cgtctccgcg agacggtcca ccgtcacacg cgcgaaggcg cgtggaagcc cgacgcctcc
     1621 gtcctgctcg agatcctgcg cgaggtcggc gccgcgccca cccaggcggt ctacgtggga
     1681 gacagcctcg tcaaggacgt cgcgatggcc caggccgctt cggtgatgga cgtcttcgcc
     1741 cgctacggcg acgtccggag ccggccgggc tacgccctgc tccgccgggt gacgcactgg
     1801 tcggccgcga tgatggcccg ctcggagggg atccgcgagg ccgacgtccg gccgactcac
     1861 gtcctgtccg gcggcttcgc cgagctgctc gggcgcatcg acttctgtcg cttcaccccg
     1921 cgctcttccg cggtctgagc catgttcgtc gagctgcacg cccggagcgc cttcagcttt
     1981 ctcgaggcgg ccgcgctgcc ggaggcgctg gccgagcggg cggccgcgct cggtcagccg
     2041 agcgtcgccc tcctcgacgc cgacggggtc tacggcgcgc cccggctcta tcgcgcctgc
     2101 acccggctcg ggctctccgc gcgggtgggc gcggaggtca ccctggccga cggcagccgt
     2161 ctcccgctgc tggtggagga ccgcgagggc tatcagaacc tctgccgcct gctcacccgg
     2221 gtcaagatgc gagccgcgaa gggcgagggc gcggccaccc tcgacgagct ctccgagttc
     2281 gcgggcgggc tcgtctgtct caccggcggc gggcgggggc cggtggcgac ggtgctcgag
     2341 ggcgaaggcg cgccggcggg ccggcgcctg ctcgagcgcc tcgtctcgat ctacggccgc
     2401 ttcaacgtct tcgcggagct gcagcgccac ctgagccgtc cggaggaagc gcgcaacgag
     2461 tggctgcgcg ccgaggcccg gcgcctgcgc ctgggcccgc tggccaccaa cgagccccgc
     2521 ctgatcgcgc gccgggaccg gcccctgctc gacgcgctca cgtgcatccg cgagcacgtc
     2581 acgctcgagg cggcgggccg gcggctcgcg cgcaacagcg agcacttcat gaagagcggg
     2641 gccaccatgg agcggctctt ctccgactgt ccggaggcgg tggcgaacac cggcgagctg
     2701 gccatgcggc tcggcttcac cctgaaggac ctgggctacc gcttcccgga ctacccgctg
     2761 ccgcgcgggg agaccccgat cggcttcctg cgtgagctcg cctacgcggg ggcgcgcacc
     2821 cgctacggct cggggccgct cgcggagaag gcgcggcggc agatcgagca cgagctggcc
     2881 ctgatcggcc ggctcgacct ggccggctac ttcctcatcg tctgggacct ggtgcagtac
     2941 tgcggcgccc acgacgtgct ggtgcagggg cgtggctccg cggccaacag cgcggtgtgc
     3001 tacgccctcg ggctcaccgc ggtggatccg gtgggcatgg agctgctctt cgagcgcttc
     3061 ctctccgagg agcgcgggga gtggcccgac atcgacctcg acctgccgag cggggaccgc
     3121 cgcgagcggg tgatccagta cgtctacgag cgctacggcc ggctgggcgc ggcgatgacc
     3181 gccaacgtga tcagctaccg cgggcgcagc gcggcccgcg aggtcggcaa ggtgctggac
     3241 ctgcccgccg acgtgatcga gcgcctctcc cagctcgtga gcaactggga gtacaacgat
     3301 cccggcgaca gcctgctcgc gcatctgggc gaggcgggct gcgatccggc cgagccgcgc
     3361 ggccagcact tcgtccggct ctggagcgcg atccaggacc tgccgcggca cctgggccag
     3421 cactccggcg gcatggtgat cgcccagggc cggctcgacg gggtggtgcc gctcgagccc
     3481 gccaccatgc ccgggcgcag cgtggtgcag tgggacaagg acgactgcgc cgcgctcggc
     3541 atcgtcaagg tggacctgct ggggctcggc atgatgtcgc tgctccagga cgcgctcgag
     3601 atgatccgcc gccgcggggg tgcggtcgac ctcgcccatc tgccccagga ccccacggtc
     3661 taccgcatgc tccagcgggc ggacaccatc ggggtcttcc aggtggagag ccgcgcccag
     3721 atggccacgc tgccgcgcct gcggcccacg tgcttctacg acctggtggt gcaggtggcg
     3781 atcatccgcc ccggccccat cgtgggcgac atggtccatc cctacctgcg ccgccgcgcg
     3841 gggcgcgagg agatcacggt gccccatccc gatctcgagc cgatcctgcg ccgcaccctc
     3901 ggggtgccgc tcttccagga gcagttgctg cgcatggcga tggccaccgc cggcttcacc
     3961 ggaggcgagg ccgaggagct gcggcgcgcc ttcggcttca agcgctcgga gcgcgccatg
     4021 aaggaggtgg agctgaagct ccgcgcgggc atggcgcgcc agggcatctc gggcgccgcg
     4081 gccgaggcca tcatccactc catcaccgcc ttcgcgctct acggcttccc ggagtgcgtg
     4141 gtgggcgaca cccgcgtcgt cgacccggag accgggcgcc gggtccgcat cgaggacgtg
     4201 gtggagggcc gcgcgtcgct gagccacacc ctggcctgtg accccgatct gaagatccgg
     4261 acgcgccggg tcctcgccgc caccgcgagc ggatcgcgga tggtgtttcg gctgcgcacc
     4321 ttcctggggc gggagctggt ggccaccccg gagcacctgc tcctcaccga ggacggctgg
     4381 cggccactgg ggagtctcca gttcgacgat cacgtggcga ccgcggaggc ggatgcgtcg
     4441 tgcgtgggct gggactgtgt gatgagcgtg gagccggcgg gaatgagccc gacgtacgac
     4501 ctcgcggtgg aggaggatca caacttcgtc gccgacgatc tggtcgtgca caactcgcac
     4561 gcggcgagct tcgcgctgct cgcctacgcg agcgcgtacc tcaaggcgca ccatccggcc
     4621 gcgttctacg ccgcgctgct caacaaccag ccgatgggct tctacgaccc ggccaccatc
     4681 gtgaacgacg cgggccggca cgggcaggtg atccaccccg ccgacgtcaa ctactcgggc
     4741 tggctgtgta cgatcgaatc catgggcgaa ccaaccggct caccggccgg cgaggtccgc
     4801 ctggggctgc gctacgtgcg cgggctgcgg gaggaggcgg gcctgcgcct cgaggcggag
     4861 cggaagcggc gggtcttcgc ctcggtcgag gacctggtca agcgcggggg gctgcgtcac
     4921 gacgaagtgg cccagctggc ccgggtgggc gcgctggcga gcctcgagcc ggagcgtcgc
     4981 gccgcgctgt gggaggccga gcgggtgggg cggccggcgg gggcgctcta cgaggagctg
     5041 gcggcgcccc cggagacctc gcccttgcgg ccgatgaccg ccgaggagcg gctggtcgcc
     5101 gattacgatg gcaccggcct caccctcggc ccgcatccga tggcgttccg gcgcggacgg
     5161 ctcctgcgaa tgggcgtgtc gcgggcggcg gatctcgccg ccttccgtca cggggcgtgg
     5221 gtgaaggtgg cgggggcggt ggtggtgcgc cagcggcccg gcaccgcgaa gggcttcgtc
     5281 ttcctcaacc tcgaggacga gaccgggctc atcaacgtga tcgtgcgccc ggcgctcttc
     5341 caccgccacc ggctggccct ggtcagcgag tcgttcctgc tgatcgaggg cacgctgcag
     5401 cggcaggacg acgtcacctc cgtgcgcgcg gagcggctcg cccccctgcg ccatcgcctg
     5461 gcgaacgtgc cttcgcacga ttttcactga ggcccgccac cgcggccagg aacacgcgca
     5521 gcgccggcgt ctccggcttg tcgcgtcggc gcaccaggcc gaggcggcgc accagggcgg
     5581 gggacagcgg gcgcatccgg agggcgcccg cccgcacctc gtcccgcacc gccatctccg
     5641 ggctcaccga gacgccgagg ccggcgccca ccagcttctt gatcgcctcc tcgttgccca
     5701 gctccatcac cactcgcggc cgcgcgccgc cgcgccggaa ccagccgtcg atcacgcgcc
     5761 ggatggtgcc gccccgctcg tagaggatga gcggctgctg cgcgagctcc gccgcggtga
     5821 gcgcgcccgg gcccggccac cggtgcccgg gcggccccac cgcgacgagc gggtcctggc
     5881 ggaacggggt cacggtgagc tgacggccgg tgaccggcag cgtgacgagg cccacgtcca
     5941 gctcgttggc ggccaccgcg gccgcgatct cgggcgcgtt gccggtgacc acgatcagct
     6001 ccagctcggg gtagcgggtc cgcagccggc gcagcagcgg aggcagcagg tggatgctcg
     6061 cggtggcgcc ggtgcccagg cggagccggc ccgccaccac cccgcggagg tggtggagcg
     6121 cctcctgagc ccggtcgagc tcggcgaagg cgcggccggc gtgctcgagc agcaccgtcc
     6181 cggcgcgggt ggggaacgcg cgcttgccca cccgctcgag cagggcggtg ccgcagcggg
     6241 cctcgagctg gcgcatgtgc aggctgaccg cgggctggct caggtgcagg ctctcggccg
     6301 cgcgcgagaa gctgccgtgg cgggcgatct cgcggagggt ccgcacgtgg gcgggattca
     6361 ggtccattcg gttcggagtc cttggcgaag tattcctcat cgtttgcatg agaacaatct
     6421 atttccctta tcgcatcgcc gcgggacaca ctgcagaaaa ggaggacgat cgtcatgggt
     6481 gacgtctcga tccgccccgc gaccctggac gacgcggcgg cgatctgcac gatctacaac
     6541 cagggcatcg aggaccgcat cgccacgctc gagaccgagc tgcgcacccc ggaggagcgc
     6601 cggcagtgga tgggcggtcg cgccgcgcgc cacccggtgg tggtcgcggc gaccgggggc
     6661 caggtggtgg gctgggggag cctcaacccg ttcaacccgc ggcccgccta cgacaacgtg
     6721 gtggacctct cggtctacgt ggagcgcggc tggcgcggcc gcggggtcgg gcgcgcgctc
     6781 ctgcagcacc tgctgccgct ggcgcggacg ctcggctacc acaagatggt gctggccacc
     6841 ttcccctaca acgaggcggg agtcgccctc taccggaaga tgggcttcag cccggtcggg
     6901 gtctaccacg agcagggccg cctcgacggc cgctgggtgg acgtgctgat catggagcgt
     6961 ctgctctgag ctacggctgg accgtcgtcg gcaccctctg cgtcaccgag atcgtctcgt
     7021 ggggcatcat gtactacggc ttcccggtgt tcctggcctc gatggaagcc gacctcggag
     7081 cctcgcgcat cgcgatcacc ggcgctttca cggtcggcat ggcggtggcc gcgctcgccg
     7141 cgctgccggt gggccgctgg ctcgaccgcc gcgggccctg ggggctgatg acggtgggct
     7201 cgtgtctcgg cgccgcgctc atggtcgcct ggtcgcaggt tcacggggtg gtggggctct
     7261 acgtgatctg gagcctgatg ggcctggcgc tggccgccac gctctacgag cccgcgttcg
     7321 gcgcggtggt ccgctggttc cccaccgacc accgcgatcg cgcgctcacc atcgtcaccc
     7381 tcgcgggcgc gctcgccagc accatcttca tgccgatcag cgccggattc ctccagcact
     7441 ggggctggcg cccggcgctg gtggggctgg cggtgatcct cgcgatcctg acgatcccgc
     7501 tccacgcggt ggtgctgcgg cggcagcc
//
LOCUS       DATMCV010000667         5231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_634993, whole genome shotgun sequence.
ACCESSION   DATMCV010000667 DATMCV010000000
VERSION     DATMCV010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634993"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VNU03_15315"
     CDS             complement(<1..804)
                     /locus_tag="VNU03_15315"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75890.1"
                     /translation="MTRIVCLLLTNELQAHSTLLQVALAHSPRVEDAGAGCIYLDASG
                     LQSLFGDDARLAERLRDAAFAAGLAIRVGVAASRLGALAAARLGAGVTVVEPGGDAAS
                     LATAPLSLLTLSEPLAIRLSRWGLRTLGELAALPTAGLFERAGAEGVQAQRLARGEDP
                     RPLASWRPVPAFEESVECEWGLETLEPVVERLSGLAAQLCARLASRGLAADGFEWVCR
                     LAGGRAHPRRAADRCRRGDGPAAARPRGPAPTRHRRRSHPAGPAHPGGGG"
     gene            complement(801..1538)
                     /locus_tag="VNU03_15320"
     CDS             complement(801..1538)
                     /locus_tag="VNU03_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75891.1"
                     /translation="MGIPIESSPPPGWEREGIDGLLGMFPPGRLSEIVGPRSSGASSL
                     LLALVARITTSGSGGSGKGGQVAFVDGMDALDPHSAAAAGADLSALLWVRCGGHLRVA
                     LSAADLLARCPGFALVVLDLDDLGPIPASACIRLQRAVEGTGTTLIVRTERHREGSPA
                     SLVLATRRLAPQWMGLRPLTRLAGLRSEISISRSRPHHSLSPLGRGQGEGPKGEGPKG
                     EGPKGEGPKGEGPQGERRWTLDWTLTQ"
     gene            complement(1612..2034)
                     /locus_tag="VNU03_15325"
     CDS             complement(1612..2034)
                     /locus_tag="VNU03_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75892.1"
                     /translation="MADRPGVLAVFFVLTWLTGLSLAAAFATSAYGFGRMSEPERTDL
                     ISLAIFGVMGLVSQAGLLLAPWAVTRGSTPRIIAALLMAPSGIFLSIFAYEGFARYAS
                     GSPIWAVAWTVYLCGVLVYVAAYVALVRGRFGPRISSS"
     gene            complement(2027..2635)
                     /gene="leuD"
                     /locus_tag="VNU03_15330"
     CDS             complement(2027..2635)
                     /gene="leuD"
                     /locus_tag="VNU03_15330"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWP75893.1"
                     /translation="MESFARLTAVAAPMDLPNIDTDRIIPARFLRRPRESGFGPLLFH
                     DVRFGADGGERADFVLNRPAYREARILVTAENFGCGSSREMAVWALLDHGIRVVIGPS
                     FGDIFFENCFKNGALAIVVPGETAAGLRDALAARPGTRLTVDLEAQTIAGADSPPIGF
                     EVDAFRKHGLLTGLDELALTLEQLPAIEAFEARRRVEMPWLG"
     gene            complement(2644..4035)
                     /gene="leuC"
                     /locus_tag="VNU03_15335"
     CDS             complement(2644..4035)
                     /gene="leuC"
                     /locus_tag="VNU03_15335"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWP75894.1"
                     /translation="MSRTMFEKIWSRHVVAKGPGGQSLLYVDRHLLHEGSTHAFGRLE
                     KKGRAVRRPEACVATADHYVPTAPGAGEIADPEVRRMVADLGRHTGRLGITFFGRGEP
                     RQGIVHVIGPEQGVTQPGILLVCGDSHTATHGAFGALAFGVGSTEVEHVLATQCLWQR
                     KPRVMRITVSGARPFGVTAKDVILSIIARIGAGGGVGYALEYAGPVIRAMSMDERMTV
                     CNMSIEAGARAGMVAPDETTFAHLEGRPFAPRGDLWDRALAHWRTLPSDPDAIFDREI
                     TLDAALIAPTVTWGTSPQDALPITERVPDPAAVGDPARRASMERALAYMGLTPGTPLA
                     GTPVSRVFIGSCTNSRLEDLRAAAGIARGRRAAVPAWVVPGSGAVKRAAEAEGLDRVF
                     TDAGFEWREPGCSMCVGMNGDTATAGERVASTSNRNFEGRQGQGARTHLMSPSMAAAA
                     AVTGRLTDVRTLG"
     gene            complement(4035..4898)
                     /locus_tag="VNU03_15340"
     CDS             complement(4035..4898)
                     /locus_tag="VNU03_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HWP75895.1"
                     /translation="MRTTTRLRQMLQAPGIIVAPGAYDGFSARLIEAAGFQAVYMTGA
                     GTAASHLGQPDLGLATLTEMANHAGHLASAVSLPVIADADTGYGNALNVVRTVREYEK
                     AGVAGLHIEDQVAPKKCGHIAGKQVIPTEEFCDKIRAASEYRTDPDFVIIARTDARAV
                     TSLDDAIDRGNRYAAAGADVIFFEAPQTEDEIRRVAREIKAPLLANMVIGGKTPAIKV
                     AELERIGFKMVIFPAVCMAAAVPAMEKSLAYLKEHGTDWQPGPVLSPMDIFRRVGFDW
                     WHGIEEKFAAR"
BASE COUNT          778 a         1892 c         1890 g          671 t
ORIGIN      
        1 accgcccccg cccgggtggg ccgggcccgc agggtgagac cttcgacgat gccgcgtggg
       61 ggccgggcct cgagggcgag ccgcagcagg cccgtcaccg cggcggcatc ggtcagcggc
      121 acggcggggg tgagcgcgcc cccccgcgag ccggcaaacc cactcgaagc cgtccgccgc
      181 gaggccgcgc gaggcgagcc gcgcgcagag ctgcgcggcc agcccgctca agcgctccac
      241 caccggctcg agggtctcga gcccccactc gcactccacc gactcctcga aggccggcac
      301 cggccgccag gacgcgagcg ggcggggatc ctccccgcgg gccagccgct gggcctggac
      361 cccctcggcg ccggcccgct cgaacagccc cgcggtgggc agcgccgcga gctctccgag
      421 cgtgcgcagc ccccagcgcg agagccggat cgccaggggc tcggagagtg tgaggagcga
      481 aagcggcgcc gtggccagcg aggccgcgtc gcctcccggc tcgaccacgg tcacgccggc
      541 tccgaggcgg gcggcggcca gcgcgccgag gcggctcgcc gcgacgccca cacgaatcgc
      601 gagccccgcc gcgaaggcgg cgtcacggag ccgctcggcc agccgggcgt cgtcgccgaa
      661 gaggctctgg agccccgagg cgtcgagata gatgcagccg gcgccggcgt cctctacccg
      721 gggcgagtga gcgagggcga cctgaaggag ggtgctgtga gcttggagct cgttcgtcag
      781 tagtagacag acgatgcggg tcattgggtc agtgtccagt cgagggtcca gcgcctctca
      841 ccctggggcc cctcaccttt gggcccctca cctttgggcc cctcaccttt gggcccctca
      901 cctttgggcc cctcaccctg ccctctcccc agaggggaga gggagtgatg cgggcgggag
      961 cgggagatgg agatctcgga gcgcaggcca gccagccggg tgagcggccg cagtcccatc
     1021 cactgggggg ccaggcgccg ggtggccagc acgagcgagg cggggctgcc ctcgcgatgc
     1081 cgctcggtcc gcacgatcag ggtggtgccg gtcccttcca cggcgcgctg gagccggata
     1141 caggccgacg cggggatggg gccgaggtcg tcgagatcca ggacgaccag ggcaaagccc
     1201 gggcagcggg cgagcagatc ggcggcgctg agcgctaccc gcaggtgacc gccacagcgc
     1261 acccaaagca gggcggagag atcggcgccc gccgcggcgg cggagtgagg gtcgagcgca
     1321 tccatcccgt ccacgaaggc cacctggccg cccttgccgc tacccccgct acccgaagtc
     1381 gtgatccggg cgacgagggc caggagcagg ctgctggctc cggaggaccg gggaccgacg
     1441 atctcgctga gccggcccgg cgggaacatg ccgagcaggc cgtcgatccc ttcgcgctcc
     1501 caccctggcg gaggtgagga ctcgatcggg atgcccatcg agaccaatta cacgtcactt
     1561 gtttagtatc tttcaacaac aagatacagg gtagccgagt ggatacaccc actagctgga
     1621 ggatatgcgg gggccgaatc gcccccggac cagggccacg taggcggcca cgtacaccag
     1681 caccccgcag aggtacaccg tccacgccac cgcccagatc ggcgatcccg aggcgtaccg
     1741 ggcgaagccc tcgtacgcga agatgctgag aaagatcccg gacggcgcca tcagcagggc
     1801 cgcgatgatc cgcggggtcg acccgcgcgt caccgcccag ggcgccagca gcaggcccgc
     1861 ctgcgagacg agccccatca ccccgaagat ggccaggctg atcaggtcgg tgcgctcggg
     1921 ctcggacatg cgcccgaagc cgtacgccga cgtcgcgaag gcggccgcga gcgacaggcc
     1981 ggtcagccag gtcagcacga agaacaccgc gagcacgccc ggacgatcag ccaagccagg
     2041 gcatctcgac gcggcggcgc gcctcgaagg cctcgatggc cggaagctgc tcgagggtca
     2101 gggccagctc gtccaggccg gtgagcagcc cgtgcttgcg gaaggcgtcc acctcgaacc
     2161 cgatcggcgg gctgtcggct cccgcgatcg tctgggcctc caggtccacc gtcaggcggg
     2221 tcccgggccg cgccgcgagc gcgtcccgca gcccggccgc ggtctcaccc gggaccacga
     2281 tggcgagcgc cccgttcttg aagcagttct cgaagaagat gtcgccgaag ctcggtccga
     2341 tcaccacccg gatgccgtgg tccaggagcg cccacaccgc catctcgcgg gaggagccgc
     2401 agccgaagtt ttccgcggtc accaggatgc gggcctcgcg ataggcgggg cggttcagca
     2461 cgaagtcggc ccgctccccg ccgtccgcgc cgaagcgcac gtcgtggaac agcagcggtc
     2521 cgaagccact ctcgcggggc cgccgcagga agcgcgcggg gatgatgcgg tcggtgtcga
     2581 tgttcggcag atccatcggc gcggccaccg cggtgagacg ggcgaacgac tccatcggtc
     2641 gcctcagccg agcgtccgca cgtcggtgag ccgcccggtg accgcggcgg ccgcggccat
     2701 cgacgggctc atgaggtggg tgcgcgctcc ctgcccctgg cggccctcga agttgcggtt
     2761 ggaggtgctg gccacgcgct cgcccgcggt cgcggtgtcg ccgttcatgc ccacgcacat
     2821 cgagcagccg ggctcgcgcc actcgaagcc cgcgtcggtg aagacccggt cgagcccttc
     2881 ggcctcggcc gcgcgcttga ccgcacccga gcccggcacc acccacgcgg gcaccgccgc
     2941 gcggcgaccg cgggcgatcc cggccgccgc gcgcaggtcc tcgagccggc tgttggtgca
     3001 cgagccgatg aacacgcggc tcaccggggt gcccgcgagc ggggtgcccg gggtcagccc
     3061 catgtaggcg agcgcacgct ccatgctcgc ccggcgcgcc ggatcgccca ccgccgcggg
     3121 atccgggacg cgctcggtga tgggcagcgc gtcctgcggg ctcgtgcccc aggtgacggt
     3181 gggcgcgatc agggccgcgt cgagggtgat ctcccgatcg aagatggcgt cgggatccga
     3241 aggcagcgtg cgccagtggg cgagcgcccg gtcccacagg tccccgcgcg gggcgaacgg
     3301 ccgaccctcg agatgggcga acgtggtctc gtcgggcgcc accatccccg cgcgggcgcc
     3361 ggcctcgatc gacatgttgc agaccgtcat gcgctcgtcc atcgacatcg cgcggatgac
     3421 cggtcccgcg tactcgagcg cgtaccctac tcccccgccc gcgccgatcc gcgcgatgat
     3481 cgacaggatc acgtccttgg ccgtcacccc gaagggccgc gccccggaaa ccgtgatccg
     3541 catcacgcgc ggctttcgct gccagaggca ctgggtggcc agcacgtgct ccacctcggt
     3601 cgagccgacg ccgaaggcga gcgccccgaa cgccccgtgg gtcgcggtgt gcgagtcccc
     3661 gcacacgagc aggatgccgg gctgggtcac tccctgctcc gggccgatca cgtggacgat
     3721 gccctgccgc ggctcgccgc gcccgaagaa cgtgatgccg agccgaccgg tgtggcggcc
     3781 gagatccgcg accatccggc gcacctcggg gtccgcgatc tccccggcac ccggagcggt
     3841 cggcacgtag tggtcggcgg tggccacgca cgcctccggc cggcgcaccg cgcggccctt
     3901 cttctccagg cgtccgaagg cgtgggtgga gccctcgtgg agcaggtggc ggtcgacgta
     3961 gagcagcgac tggccgcccg gccccttcgc caccacgtgg cggctccaga tcttctcgaa
     4021 catcgtgcgg gacatcagcg cgccgcgaac ttctcctcga tcccgtgcca ccagtcgaag
     4081 ccgacgcggc ggaagatgtc catcgggctc agcaccgggc cgggctgcca gtcggtgccg
     4141 tgctccttca ggtacgcgag cgacttctcc atcgcgggca ccgcggccgc catgcacacc
     4201 gccgggaaga tgaccatctt gaagccgatg cgctcgagct cggccacctt gatcgcgggg
     4261 gtcttgccgc cgatgaccat gttggccagc agcggggcct tgatctcgcg ggccacccgg
     4321 cggatctcgt cctcggtctg gggcgcctcg aagaagatca cgtcggcgcc cgccgccgcg
     4381 tagcggttgc cgcggtcgat ggcgtcgtcg agcgaggtga ccgcgcgggc gtcggtgcgg
     4441 gcgatgatga cgaagtcggg atcggtgcgg tactccgagg cggcgcggat cttgtcgcag
     4501 aactcctcgg tcgggatcac ctgcttgccc gcgatgtgcc cgcacttctt gggggccacc
     4561 tggtcctcga tgtggagccc ggccactccc gccttctcgt actcgcgcac cgtgcgcacc
     4621 acgttgagcg cgttgccgta tccggtgtcg gcgtccgcga tcaccggcag ggagaccgcg
     4681 gaggcgagat gaccggcgtg gttggccatc tcggtcagcg tggccaggcc caggtcgggc
     4741 tggcccaggt gcgaggcggc ggtgcctgcg ccggtcatgt acaccgcctg gaagccggcc
     4801 gcctcgatca ggcgcgcgga gaagccgtcg taggcgcccg gggcgacgat gatgccggga
     4861 gcctggagca tctggcggag tcgggtcgtg gttcgcatga cggaaaacct ttcccgggcg
     4921 ttcggcccga cggggacggc gtggccaccg agggctcgga ggcgaagccg attgtcggac
     4981 agggggctcg cgcttgtcaa atgcggccgg ttctgcgcgc caccggagca acaatggccg
     5041 gagcgacgtc accacgatcc caagcgaggg cccggtccat gcgacagatg acgctgacct
     5101 tctttccgat gctgatcctc gtcctctccc cggtcggccc ggcccgaggg gacgaggtgc
     5161 tgttccagaa cggcgaccgg ctgtccggca agatcctcaa ggcggccggc ggcaagctca
     5221 cgatcaagac c
//
LOCUS       DATMCV010000668         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_903842, whole genome shotgun sequence.
ACCESSION   DATMCV010000668 DATMCV010000000
VERSION     DATMCV010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903842"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2079)
                     /locus_tag="VNU03_15345"
     CDS             complement(<1..>2079)
                     /locus_tag="VNU03_15345"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWP75896.1"
                     /translation="DLGREAFIARVWQWKEESGGTIIRQLKRLGASCDWARERFTMDA
                     GLSAAVREVFVRLWEEGLIYQGDYIVNWCPRCQTVLSDLEVEREDQDAEFVYIKYGPL
                     TLGTVRPETKLGDTAVAVHPKDKRYKKYVGKTLEVPSVEGNITLKVVADEAVDPKFGT
                     GVIKVTPGHDPTDFEIGKRHNLPIRSVIGFDGKMTELAGKYAGLDRFETRKRIVEDMK
                     QLGLIDRIEPYRHSVGVCYRCKTVVEPLVSKQWYVRTKPLAEPAIKAVRSGKIKIVPR
                     AWSKTYFHWMENIRDWTISRQLWWGHRIPVWYCDKDGSMHVSRTDLTACPQCGGALRQ
                     DTDVLDTWFSSGLWPFSTLGWPQETPELKVFYPTSVLSTGFDILFFWVARMAMLGIKF
                     MGDVPFRHVYIHALIRDAEGQKMSKSKGNVVDPLSVMDKYGTDAFRFTLAALAAQGRD
                     IRLAEERIEGYRNFANKIWNAARLVLTNLDGYDPALARRGTPSVADRWIKSRLTEATA
                     RVRKAIDSYRFNDAASAVYQFLWHEYCDWYLEIAKRSLYQPESPAARAVTQRTLVETL
                     EATLRLLHPFMPFISEELWQRLPHPGDSIPAPARSIMIAPFPKASRKGRDPEAERLMA
                     PVLDFVSAIRTIRSESRISPAVELAVSVNPAGPAVVAALASGAAVIGSLARAAITVSA
                     DGARPANSAMA"
BASE COUNT          316 a          702 c          720 g          341 t
ORIGIN      
        1 gccatcgcgg agttggccgg gcgggctcca tcggcggaca ccgtgatcgc ggcccgggcc
       61 aggctcccga tcaccgccgc gccggacgcg agcgcggcga ccaccgccgg gcccgccggg
      121 ttcacactca ccgccagctc cactgcgggc gagatccggc tctcgctccg gatggtccgg
      181 atcgcgctca cgaagtcgag caccggcgcc atgagccgct ccgcctccgg atcgcgcccc
      241 ttgcgggagg ccttcgggaa cggggcgatc attatggacc tggcgggggc cgggatcgaa
      301 tcgcccgggt gcggcagccg ctgccacagc tcctcggaga tgaacggcat gaacgggtgg
      361 aggagccgca gcgtcgcctc gagcgtctcc accagcgtgc gctgggtgac cgcgcgggcg
      421 gccgggctct ccggctggta gagactccgc ttggcgatct cgagatacca gtcgcagtac
      481 tcgtgccaga ggaactggta gaccgcggag gccgcgtcgt tgaaccggta ggagtcgatc
      541 gccttgcgca cccgggcggt ggcctcggtg aggcggctct tgatccagcg atcggccacc
      601 gacggggtgc cgcgccgggc gagcgctgga tcgtagccgt ccaggttggt cagcaccagc
      661 cgggccgcgt tccagatctt gttggcgaag ttccggtagc cctcgatccg ctcctcggcg
      721 aggcggatgt cgcggccctg cgccgcgagc gcggccagcg tgaagcggaa cgcgtcggta
      781 ccgtacttgt ccatgacgct caggggatcg accacgttcc ccttcgactt cgacatcttc
      841 tggccctcgg cgtcacggat cagggcatgg atgtagacgt ggcggaacgg gacgtcgccc
      901 atgaacttga tgccgagcat cgccatccgg gccacccaaa agaacaggat gtcgaaaccg
      961 gtggagagca ccgaggtggg atagaagacc ttgagctcgg gggtctcctg gggccagccg
     1021 agcgtcgaga acggccagag ccccgaggag aaccacgtgt cgagcacgtc ggtgtcctgg
     1081 cggagcgctc ccccgcactg cgggcacgcg gtgagatcgg tgcgggagac gtgcatgctc
     1141 ccgtccttgt cgcagtacca caccgggatg cggtggcccc accagagctg gcgcgagatc
     1201 gtccagtcgc ggatgttctc catccagtgg aagtaggtct tgctccacgc gcgcgggacg
     1261 atcttgatct tgccggagcg caccgccttg atcgcgggct cggcgagcgg cttggtccgc
     1321 acgtaccact gcttggagac gagcggctcg accaccgtct tgcagcggta gcagaccccg
     1381 accgagtggc ggtagggctc gatgcggtcg atgaggccga gctgcttcat gtcctccacg
     1441 atgcgcttgc gggtctcgaa gcggtcgagc cccgcgtact tgccggccag ctcggtcatc
     1501 ttgccgtcga agccgatgac gctgcggatg ggcaggttgt gccgcttgcc gatctcgaag
     1561 tcggtcgggt cgtgaccggg ggtgaccttg atgaccccgg tcccgaactt cggatcgacc
     1621 gcctcgtcgg cgacgacctt gagggtgatg ttcccctcca ccgagggcac ctcgagcgtc
     1681 ttgcccacgt acttcttgta gcgcttgtcc ttggggtgca ccgccaccgc ggtgtcgccg
     1741 agcttggtct ccggccgcac ggtgccgagg gtgagcgggc cgtacttgat gtagacgaac
     1801 tccgcgtcct ggtcctcgcg ctcgacctcc agatccgaca gcacggtctg gcagcgcggg
     1861 caccagttca cgatgtagtc gccctggtag ataagcccct cttcccacag ccgcacgaag
     1921 acctcgcgca ccgcggccga gaggcccgcg tccatcgtga agcgctcgcg ggcccagtcg
     1981 caggaggcgc cgagccgctt gagctggcgg atgatggtgc cgcccgactc ctccttccac
     2041 tgccagaccc gggcgatgaa cgcctcccgc ccgagatcc
//
LOCUS       DATMCV010000669         3196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_32929, whole genome shotgun sequence.
ACCESSION   DATMCV010000669 DATMCV010000000
VERSION     DATMCV010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32929"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /gene="ilvN"
                     /locus_tag="VNU03_15350"
     CDS             complement(<1..229)
                     /gene="ilvN"
                     /locus_tag="VNU03_15350"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWP75897.1"
                     /translation="MLVENKFGVLSRVAGLFSARGYNIESLSVGETLDPSVSRMTLVV
                     RGDAFVIEQVIKQLHKLIDVIKVTDLSEENHV"
     gene            complement(449..2203)
                     /gene="ilvB"
                     /locus_tag="VNU03_15355"
     CDS             complement(449..2203)
                     /gene="ilvB"
                     /locus_tag="VNU03_15355"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HWP75898.1"
                     /translation="MKLSGARIVLECIKREGVDLIFGLPGGAVLPIYDALYDFEGLRH
                     VLVRQEAAAGHAAEGYARTTGRVGVCLVTSGPAATNLVTALQDALMDSIPIVAFTGQV
                     PTHWIGSDAFQEADNVGITRSATKHNFLVKDGKDLAPIIKEAFHIAATGRPGPVHVDL
                     PKDILVKEWNFEYPDKVHLRSYNPTYEGHPGQIKKAARSMVRAKRPVLYVGGGAISSD
                     ASPELRELADLTQIPLTQTLMGLGSFPMADPLSLDMLGMHGTYYANMAVHHSDCLIAV
                     AARFDDRVTGRVDAFSPNAEIIHIDIDPSSIAKNIKVDIPIVGDCKRVLRRLVEAVRE
                     ELKDGVPAAVTEGRRQWAAQIAEWKRDFPLRYDWDDEVIKPQYVIQELSNLTNGEAHV
                     VTGVGQHQMWAAQYYRFKHPRRWCTSGGLGTMGYGLPTAMGVQAAHPGKLVLNIDGDG
                     SFAMNSQELATCFTENLPVKTIIINNSGHGMVRQWQRIIYNERYFAIDLPGIPDWVKL
                     AEAYGCVGIRVTKPSEVVPALEKMLSTPAPVVMDVCVDKDECVFPMVPAGGANRDMIL
                     APPTREIREKAAKSQTGF"
     gene            complement(2200..>3196)
                     /locus_tag="VNU03_15360"
     CDS             complement(2200..>3196)
                     /locus_tag="VNU03_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HWP75899.1"
                     /translation="KKAVDRLLPFQREDFIGIFRAMAPHPVTIRLLDPPLHEFLPKYK
                     EVLEEYTRLDARGFNPERHAELGALKARLEALQEANPMLGHRGCRLGITFPEIYDMQV
                     RAIMEAACQVAGEGFSVEPEIMIPLTGTVAEMKLTREMTDRVARQVVAETGRDVRYSV
                     GTMIEVPRAALVADRIAEHAEFFSFGTNDLTQLTYGYSRDDIGKFLPFYLEKKLLPHD
                     PFAVLDQRGVGELVRIGIERGRQTVPTLKVGICGEHGGEPSSVEFCHRVGMTYVSCSP
                     YMVPVAWLAAAQGEIKEPRPPRKASRALHGEVQPKASRGNTKRSHVSSTKRGKKR"
BASE COUNT          462 a         1110 c         1058 g          566 t
ORIGIN      
        1 cgacgtgatt ctcctcgctg aggtcggtca ccttgatgac gtcgatcagc ttgtgcagct
       61 gcttgatcac ctgctcgatg acgaaggcgt cccctcgcac caccagggtc atgcgcgaca
      121 ccgagggatc gagggtctcc cccaccgaca ggctctcgat gttgtacccc cgcgccgaga
      181 acagcccggc cacccgggag agaacgccga acttgttctc gacgagcacc gcgatcgtat
      241 gcttttcgat gcttcctgtt tctgccacgt cagccgtcct ttcctacgtg ctctggcccg
      301 tgctgcgtcc acgcctggct acgcgtggtg ggcggacgct cggacccgcc cctctattta
      361 atgcgggggc ccagaaatgg cccccgcact cccccaggcc gcggacgctc gttccgagag
      421 gttcggcccg ccggcgctgt cgcatcgtct agaagccggt ttgggacttg gccgccttct
      481 cgcggatctc gcgggtgggc ggggccagga tcatgtcgcg gttggcgccg ccggcgggca
      541 ccatcgggaa cacgcactcg tccttgtcca cgcagacgtc catcaccacc ggcgcgggtg
      601 tgctcagcat cttctcgagc gcgggcacca cctcgctcgg cttggtcacc cggatgccga
      661 cgcagccgta cgcctcggcc agcttcaccc agtccgggat gccgggcagg tcgatcgcga
      721 agtagcgctc gttgtagatg atgcgctgcc actggcgcac catgccgtgg ccgctgttgt
      781 tgatgatgat ggtcttgacc ggcaggttct ccgtgaaaca ggtggcgagc tcctggctgt
      841 tcatcgcgaa ggagccgtcg ccgtcgatgt tgagcacgag cttgcccggg tgcgcggcct
      901 gcacccccat cgcggtcggc agaccgtatc ccatggtgcc gaggccgccc gaggtgcacc
      961 agcgacgcgg gtgcttgaac cggtagtact gggcggccca catctggtgc tggccgaccc
     1021 cggtgaccac gtgcgcctcg ccgttggtga ggttcgagag ctcctggatc acgtactggg
     1081 gcttgatgac ctcgtcgtcc cagtcgtagc gcagcgggaa atcgcgcttc cactccgcga
     1141 tctgggccgc ccactggcgc cgtccctcgg tcaccgcggc gggcaccccg tccttcagct
     1201 cctcccgcac cgcctccacc agccggcgca gcacccgctt gcagtcgccc acgatcggga
     1261 tgtcgacctt gatgttcttg gcgatcgacg agggatcgat gtcgatgtgg atgatctccg
     1321 cgttcggcga gaacgcgtcc acccggccgg tcacccgatc gtcgaagcgc gcggccaccg
     1381 cgatcaggca gtcggagtgg tgcaccgcca tgttggcgta gtaggtgccg tgcatgccga
     1441 gcatgtcgag cgagagggga tcggccatcg ggaacgagcc caggcccatc agggtctggg
     1501 tcagcgggat ctgggtcagg tcggccagct cgcgcagctc gggagaggcg tcggaggaga
     1561 tggcgccgcc gcccacgtag agcaccgggc gcttggcccg caccatcgag cgggccgcct
     1621 tcttgatctg tccgggatgc ccctcgtagg tcggattgta cgagcgcagg tgcaccttgt
     1681 ccgggtactc gaagttccac tccttgacca ggatgtcctt cggcaggtcc acgtggaccg
     1741 gccccgggcg tccggtggcc gcgatgtgga acgcctcctt gatgatgggg gccaggtcct
     1801 tgccgtcctt gaccaggaag ttgtgcttgg tggccgagcg ggtgatgccg acgttgtcgg
     1861 cctcctggaa ggcgtcgctg ccgatccagt gggtcggcac ctggccggtg aaggccacga
     1921 tcgggatcga gtccatcagg gcgtcctgca gcgcggtcac caggttggtc gcggccggcc
     1981 ccgaggtgac gaggcagacg ccgactcgac cggtcgtccg ggcgtagcct tccgccgcgt
     2041 gcccggcggc ggcctcctga cgtaccagga cgtgacgcag cccctcgaag tcgtacaggg
     2101 cgtcgtagat cggcaggacc gcgccgcccg gcagaccgaa gatgagatcc acgccttccc
     2161 gcttgatgca ctcgaggacg atccgggcgc ctgagagctt caccgcttct tccccctttt
     2221 cgtgctgctc acgtgacttc tcttcgtatt ccctcggctc gccttcggct gcacctcgcc
     2281 atgcaaggcc cggctcgcct tgcgcggggg ccgaggctcc ttgatctcac cctgggccgc
     2341 cgcgagccac gcgaccggga ccatgtaggg cgagcaggag acgtaggtca tcccgacccg
     2401 gtggcagaac tccaccgacg agggctcacc cccgtgctcc ccgcagatcc cgaccttcag
     2461 cgtgggcacg gtttgccggc cccgctcgat gccgatccgt accagctccc ccacgccccg
     2521 ctgatccagc accgcgaacg ggtcgtgggg cagcagcttc ttctccagat agaacggcag
     2581 gaacttgccg atgtcgtcgc ggctgtagcc gtacgtcagc tgggtcaggt cgttggtgcc
     2641 gaacgagaag aactcggcgt gctccgcgat ccggtccgcg accagcgcgg cgcgcggcac
     2701 ctcgatcatg gtgccgaccg agtagcgcac gtcccggccg gtctccgcca ccacctggcg
     2761 agcgacccgg tccgtcatct cgcgggtcag cttcatctcc gccaccgtgc cggtgagcgg
     2821 gatcatgatc tcgggctcga ccgagaaccc ctccccggcc acctggcagg ccgcctccat
     2881 gatcgcccgc acctgcatgt cgtagatctc ggggaaggtg atgccgaggc ggcagccccg
     2941 gtgcccgagc atggggttcg cctcctggag cgcctcgagc cgcgccttca gcgcgcccag
     3001 ctccgcgtgc cgctccgggt tgaagccgcg cgcgtcgagc cgcgtgtact cctcgagcac
     3061 ctccttgtac ttcgggagga actcgtggag cggcggatcg agcagccgga tggtgaccgg
     3121 gtgcggggcc atggcgcgga agatgccgat gaagtcctcg cgctggaacg ggagcaggcg
     3181 atcgaccgcc ttcttc
//
LOCUS       DATMCV010000670         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_972099, whole genome shotgun sequence.
ACCESSION   DATMCV010000670 DATMCV010000000
VERSION     DATMCV010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972099"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..903
                     /locus_tag="VNU03_15365"
     CDS             91..903
                     /locus_tag="VNU03_15365"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP75900.1"
                     /translation="MPLTSLKDRTAVVTGGASGIGRALSLLFAREGTHVVVADLDEAG
                     MAETVAGVSRAGRRGLAVKTDVSRLADVETLADRAFGEFGAVHVVCNNAGVALWGGLE
                     SVTHKDWEWAMGVNLWGVIHGVEAFVPRMVAGKQPGHVVNTASMAGLIASQGLGIYNT
                     TKYAVVGLSETLQKDLRGYDIGVSVLCPMGVNTQIRQSDRNRPAALRNEAAEGEGRAV
                     ELIGRYLPPEHVAERVLRAIYANRLYVITHEEGLTPLKRRFERMAQAIEENK"
     gene            900..1823
                     /locus_tag="VNU03_15370"
     CDS             900..1823
                     /locus_tag="VNU03_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HWP75901.1"
                     /translation="MKFSVIEGWGKLPEGFTYHEVAGVAVDKQDRAFVFNRGEHPVIV
                     FDRDGNFLRSWGEGVIRRAHGITIDADDMVWLTDDLHHTVRKFTPEGKHLLTIGDPDS
                     PAELHGGRPFNRPTHVAICPKSGFLFVSDGYGNSRVHKYSPDGKHVMSWGEAGTDPGQ
                     FNLPHNLVTDRDGLVYVADRENHRVQIFDGKGGYQGQWNNLHRPCGLFADRGHGLFFV
                     GELGSGMPVTEKTPNLGPRVSVLDAKGQRIARFGGQFASEKPGEFIAPHGLVVDSRGD
                     VYVGEVSWTGYGQFLTPPRVMRSFQKFKRAD"
     gene            complement(1820..>2193)
                     /locus_tag="VNU03_15375"
     CDS             complement(1820..>2193)
                     /locus_tag="VNU03_15375"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWP75902.1"
                     /translation="LRVRTFRAGGADGPANWEVLSPIVIQGRFEGPPGAAHGGIVAAY
                     LDEILAGAAVSHTGRLYVTGELTVRYVKGTPIERPLLGRGRAVRDWDKYIDLEATIED
                     WQTQEVVAKATARFLPIRDAS"
BASE COUNT          343 a          766 c          768 g          316 t
ORIGIN      
        1 gaaccggcgc gcgacgacga tcgtgcggtc gggcatggcg gccatcctct cacttgacag
       61 ccctcgatgc caggcgcgac actcggcgcc gtgcctctca ccagcctgaa ggaccggacc
      121 gcggtggtca ccggcggcgc cagcggcatc ggacgtgccc tctcgctgct cttcgcccgc
      181 gagggcaccc acgtggtggt cgccgatctc gacgaggccg gcatggccga gacggtggcg
      241 ggggtgagcc gggccggccg gcgcggcctc gcggtgaaga ccgacgtgag ccggctcgcc
      301 gacgtggaga ccctggccga tcgcgcgttc ggcgagttcg gcgcggtcca cgtggtgtgc
      361 aacaacgcgg gcgtcgccct ctggggcggg ctcgagtcgg tgacccacaa ggactgggag
      421 tgggcgatgg gcgtcaacct ctggggcgtc atccacgggg tcgaagcctt cgtgccgcgc
      481 atggtggccg gcaagcagcc cgggcacgtc gtgaacaccg cctcgatggc cggcctcatc
      541 gcctcgcagg gcctcgggat ctacaacacc accaagtacg cggtggtcgg cctctcggag
      601 acgctgcaga aggacctgcg cggctacgac atcggggtct cggtgctctg cccgatgggc
      661 gtgaacaccc agatccggca gagcgaccgc aaccgtccgg ccgcgcttcg caacgaggcg
      721 gcggagggtg agggacgcgc ggtcgagctg atcggccgct acctgccgcc cgagcacgtg
      781 gccgagcgcg tcctgcgcgc gatctacgcc aaccgcctct acgtcatcac ccacgaggag
      841 ggtctgaccc cgctcaagcg ccgcttcgag cgcatggccc aggccatcga ggagaacaaa
      901 tgaagttctc ggtcatcgag ggctggggaa agctaccgga gggcttcacc tatcacgagg
      961 tcgcgggcgt ggcggtggac aagcaggatc gcgcgttcgt gttcaaccgg ggcgagcacc
     1021 cggtcatcgt cttcgaccgc gacggcaact tcctgcgctc gtggggcgag ggcgtgatcc
     1081 gccgggccca cggcatcacc atcgacgccg acgacatggt gtggctcacc gacgacctcc
     1141 accacaccgt gcgcaagttc acccccgagg gcaagcacct gctgaccatc ggcgatccgg
     1201 actcaccggc cgaactgcac ggtggtcggc cgttcaatcg gccaactcac gtcgcgatct
     1261 gccccaagag cggcttcctg ttcgtctccg acggttacgg caactcccgc gtgcacaagt
     1321 actcgccgga cggcaagcac gtgatgtcgt ggggcgaggc ggggaccgat cccggccagt
     1381 tcaacctgcc ccacaacctg gtgaccgacc gcgacgggct cgtttacgtg gccgaccgcg
     1441 agaaccaccg ggtgcagatc ttcgacggca agggcggcta ccagggccag tggaacaacc
     1501 tccaccgtcc ctgcggactc ttcgccgacc gcggccacgg cctcttcttc gtgggcgagc
     1561 tcggctccgg gatgccggtg accgagaaga ccccgaacct ggggccgcgt gtgagcgtgc
     1621 tcgacgccaa gggccaacgg atcgcgcgct tcggggggca gttcgcgagc gagaagccgg
     1681 gggaattcat cgccccccac ggcctggtgg tcgactcgcg cggggacgtc tacgtcggag
     1741 aggtctcctg gaccggctac gggcagttcc tcaccccgcc gcgggtgatg cggtccttcc
     1801 agaagttcaa gcgcgcggac tagctcgcgt cgcggatcgg caggaagcgg gcggtcgcct
     1861 tcgcgaccac ctcctgcgtc tgccagtcct cgatggtggc ctccagatcg atgtacttgt
     1921 cccagtcgcg caccgcccgt ccgcgcccga gcagcggccg ctcgatcggg gtgcccttga
     1981 cgtagcgcac ggtgagctcg ccggtcacgt agagccggcc ggtgtggctg accgccgcgc
     2041 cggccaggat ctcgtcgaga taggcggcca cgatgccgcc gtgggcggcg ccgggcgggc
     2101 cctcgaagcg gccctggatc acgatcggcg agaggacctc ccaattggcg gggccgtctg
     2161 ccccgccggc gcgaaacgtc cggacccgga gac
//
LOCUS       DATMCV010000671         3811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_699159, whole genome shotgun sequence.
ACCESSION   DATMCV010000671 DATMCV010000000
VERSION     DATMCV010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699159"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /gene="eno"
                     /locus_tag="VNU03_15380"
     CDS             complement(<1..718)
                     /gene="eno"
                     /locus_tag="VNU03_15380"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285777.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWP75903.1"
                     /translation="MSEIRATHAREVLDSRGNPTVEVEMWLESGAFGRAIVPSGASTG
                     KREAVELRDDDDNRYGGKGVRRAVQNVNEVIAPEIEGLEAGEQAALDRALLELDGTPN
                     KSGLGANALLGISLAAARASADDAGLPLYQYLGGPGARILPVPLMNVLNGGAHADNGL
                     DIQEFMLVPAGAGSFSEALRMGVEIFHMLKRLLKDKGLSTGVGDEGGFAPALGGNEPA
                     LDFLLRAVERSGYRPGEDVWL"
     gene            complement(722..1588)
                     /locus_tag="VNU03_15385"
     CDS             complement(722..1588)
                     /locus_tag="VNU03_15385"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HWP75904.1"
                     /translation="MKERAAAVDVALDRFLPPETELPESLHKAMRYSVFAGGKRLRPV
                     LVIAGAEAVGGSAERVMPTACALELIHTYSLVHDDLPAMDNDDFRRGVPTNHNVFGEA
                     TAILAGDALLTLAFRLVADNARATSLDALQAVVVDIADAAGHGGMVAGQVADLEAEGR
                     QVGADTVDYIHVHKTGALIRTSLRVGAMLCGATSEQVRALSVAGADLGLAFQIVDDIL
                     DVVASSEELGKTAGKDQIQQKATYPAIHGIEPSRARARFLVGEAEEALSSFGPRAEPI
                     RALGRFILERKA"
     gene            complement(1609..1848)
                     /locus_tag="VNU03_15390"
     CDS             complement(1609..1848)
                     /locus_tag="VNU03_15390"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWP75905.1"
                     /translation="MTDIKFEAALQRLEQIVDQLEAGNLSLEESLQVFEEGVGLARRA
                     GKYLDEAEKRIELLTRDESGVLKTTPFDLADEERA"
     gene            complement(1829..3187)
                     /gene="xseA"
                     /locus_tag="VNU03_15395"
     CDS             complement(1829..3187)
                     /gene="xseA"
                     /locus_tag="VNU03_15395"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWP75906.1"
                     /translation="MSERVVCTVGELAMRIKAQLEDQFPAVWVEGEISNLRTPSSGHA
                     YFTLKDDTAQLRCVLFRGRGRRVAFQPEDGMQVLAFGGLDVYLARGEYQLVVELLEPK
                     GVGALQLAFEQLKRRLEAEGLFDAARKRPLPPFPRTIGIVTSPTGAAIRDMLHVIDRR
                     FADLRILITPVRVQGEEAPGEIVAALRDLQAVEDLDVIIVGRGGGSIEDLWAFNDERV
                     ARAIAGCRVPVISGVGHETDFTIADFVADLRAPTPSAAAEVVVQEKLQVARALVGLYE
                     ALKQAMASRLERDRERVEVLGKRRVLTDAARALRDLYRRVDELTSRLTRAVRGSERQA
                     THRLSLARNALRSLNPVARIANGTVLLAQLRGRLASAAVHSVKVSQHRFDAAVGRLDS
                     LSPLAVLGRGYSLTRLLPSGVIVRDAAQTRPGDAIEILLHQGAVEARVERLKERDDRH
                     QV"
     gene            complement(3184..3651)
                     /locus_tag="VNU03_15400"
     CDS             complement(3184..3651)
                     /locus_tag="VNU03_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HWP75907.1"
                     /translation="MEAIVSYSPSPLWGEGRVRGGRTTSAVCESPRDYARGLRRRQTD
                     AERRLWARLRDRGLLGAKFARQVPIGAYVVDLCCRELKVIVELDGGQHAVQARSDTER
                     TIFLEALGYRVLRFWNNDALANTDGVLQRIAQALAARPSPRPSPQRGEGEGRE"
     gene            complement(3675..>3811)
                     /locus_tag="VNU03_15405"
     CDS             complement(3675..>3811)
                     /locus_tag="VNU03_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75908.1"
                     /translation="AAKARAITPVPGGVGPMTIAMLMRNTYRAASRLLPPLPSGERAG
                     "
BASE COUNT          532 a         1385 c         1357 g          537 t
ORIGIN      
        1 cgagccacac gtcctcgccg gggcggtagc cgctccgctc caccgcgcgg agcaggaagt
       61 cgagcgcggg ctcgttgccg ccgagcgcgg gggcgaagcc gccctcgtcg ccgactccgg
      121 tggagagccc cttgtccttc agcagacgct tgagcatgtg gaagatctcc acgcccatcc
      181 ggagcgcctc gctgaaggag ccggcgccgg cgggcaccag catgaactcc tggatgtcga
      241 ggccgttgtc ggcgtgcgcg ccgccgttca gcacgttcat gagcggcacc ggcaggatgc
      301 gcgcgccggg cccgccgagg tactggtaga gcggcaggcc cgcatcgtcg gcggaggcgc
      361 gcgcggcggc cagcgagatg ccgagcagcg cgttggcccc gaggcccgac ttgttggggg
      421 tgccgtccag ctcgagcagc gcccgatcga gcgcggcctg ctcgcccgcc tcgagcccct
      481 cgatctcggg cgcgatcacc tcgttgacgt tctgcaccgc gcggcggacg cccttgccgc
      541 cgtagcggtt gtcgtcgtcg tcgcgcagct cgaccgcctc gcgcttgccg gtggaggcgc
      601 ccgacggcac gatggcccgg ccgaaggcgc cggactcgag ccacatctcg acctcgacgg
      661 tggggttgcc ccgggagtcg agcacttcac gggcgtgggt ggcgcggatc tcggacatgg
      721 ctcaggcctt ccgctcgagg atgaagcgcc cgagggcgcg aatgggctcg gcccgcgggc
      781 cgaacgagga gagagcttcc tcggcctcgc cgaccaggaa gcgggcgcgg gcgcgcgaag
      841 gctcgatgcc gtggatcgcc gggtaggtcg ccttctgctg gatctgatcc ttgcccgcgg
      901 tcttgcccag ctcctccgag ctggcgacca cgtccaggat gtcgtcgacg atctggaacg
      961 ccaggcccag gtccgctccg gccacgctga gcgcgcgcac ctgctcggac gtcgccccgc
     1021 agagcatcgc gccgacgcgg agcgaggtgc ggatgagggc gccggtcttg tggacgtgga
     1081 tgtaatccac cgtgtccgcg cccacctgtc ggccctcggc ctcgagatcg gccacctgcc
     1141 cggccaccat gccgccgtgg ccggccgcgt ccgcgatgtc caccacgacg gcctgcagcg
     1201 cgtcgaggct ggtggcgcgc gcgttgtcgg ccaccagccg gaaggccagg gtcagcagcg
     1261 cgtcgccggc caggatcgcg gtggcctcgc cgaagacatt gtggttggtg gggacgccgc
     1321 ggcggaagtc gtcgttgtcc atcgcgggca ggtcgtcgtg caccagcgag tacgtgtgga
     1381 tcagctcgag cgcgcacgcg gtcggcatga cccgctccgc ggacccgccc accgcctccg
     1441 cccccgcgat gaccagcacc gggcgcaggc gcttgccccc cgcgaacacg ctgtagcgca
     1501 tcgccttgtg caggctttcc gggagctcgg tctcgggcgg gaggaagcgg tcgagggcca
     1561 cgtccaccgc ggcggcccgc tccttcatgt acgcgcccag gtcgaagctc acgcgcgctc
     1621 ctcgtcggcc aggtcgaagg gggtcgtctt cagcaccccg ctctcgtccc gcgtgagcag
     1681 ctcgatgcgc ttctcggcct cgtcgaggta cttgccggcg cgccgggcga ggccgacgcc
     1741 ttcctcgaac acctgcaggg attcttccag ggagagatta cccgcctcga gctgatccac
     1801 gatctgctcg aggcgctgca gcgccgcttc aaacttgatg tcggtcatcc cgttccttga
     1861 ggcgctccac ccgggcctcg accgcgccct ggtgcagcag gatctcgatg gcgtccccgg
     1921 ggcgtgtctg cgcggcgtca cggacgatca cgccggaagg cagcagccgg gtcaggctat
     1981 aaccccgtcc gagcacggcc agcggcgaga ggctgtcgag ccggcccacc gcggcgtcga
     2041 accgatgctg cgacaccttc acgctgtgga cggccgcgga ggcgagccga ccgcgcagct
     2101 gggcgagcag caccgtgccg ttggcaatcc tagccaccgg attgagagag cgcaaggcgt
     2161 ttctggccag cgagaggcga tgcgtggcct gccgctcgct cccccgcacc gcccgcgtca
     2221 ggcgcgaggt cagctcgtcc acccggcggt agagatcgcg gagggcgcgc gcggcgtcgg
     2281 tgagcacgcg gcgcttgccg agcacctcca cccgctcgcg atcgcgctcg agacggctcg
     2341 ccatcgcctg cttgagcgcc tcgtagaggc ccacgagcgc gcgcgccacc tggagcttct
     2401 cctgcaccac cacctccgcc gccgccgacg gggtcggcgc gcgcaggtcg gccacgaagt
     2461 cggcgatcgt gaagtcggtc tcgtggccga cccccgagat gaccggcacg cggcagcccg
     2521 cgatcgcgcg cgcgacccgc tcgtcgttga aggcccacag atcctcgatg gagccgcccc
     2581 cgcgcccgac gatgatcacg tcgaggtcct cgaccgcctg cagatcccgg agggcggcga
     2641 cgatctcgcc cggcgcctcc tccccctgca cgcgcaccgg ggtgatcagg atgcgcaggt
     2701 cggcgaagcg gcgatcgatg acgtgcagca tgtcgcggat ggcggccccg gtcggcgagg
     2761 tcacgatgcc gatggtgcgc gggaacgggg gcagcgggcg cttgcgggcc gcatcgaaga
     2821 gcccctccgc ctcgagccgg cgcttgagct gctcgaaggc gagctggagt gcgcccaccc
     2881 ccttgggctc gagcagctcg acgaccaact ggtattcccc gcgcgcgagg tagacgtcga
     2941 ggccgccgaa ggccagcacc tgcatgccgt cctccggctg gaaggccacg cggcgcccgc
     3001 gcccgcggaa cagcacgcag cggagctgcg cggtgtcgtc cttcagcgtg aagtaggcgt
     3061 gtcccgacga cggggtccgc aggttgctga tctcgccctc cacccacacc gcggggaact
     3121 ggtcctcgag ctgcgccttg atgcgcatcg ccagctcccc gaccgtgcag acgacccgct
     3181 cgctcattcc cgcccttccc cctctcccct ctggggagag ggccggggtg aggggcgagc
     3241 agccagcgcc tgcgcgatac gctgcagcac gccatcggta ttggccagcg cgtcgttgtt
     3301 ccagaagcgc aacacgcgat agccgagcgc ttcgaggaag atggttcgct ccgtgtcaga
     3361 ccgagcctgc accgcgtgct ggccaccgtc gagctcgacg atcaccttca gctctcggca
     3421 gcagagatcg acgacgtacg cgccgatagg cacctggcgg gcgaatttcg cgcccagcag
     3481 cccccggtca cggagccggg cccatagccg gcgctccgcg tcggtctggc gacggcggag
     3541 gccgcgggcg tagtcgcggg gggattcgca gactgcgcta gtcgtccgcc cgcccctcac
     3601 cctgccctct ccccagaggg gagaggggga gtaggagacg atcgcctcca tcgggcccct
     3661 caccaccagg cccctcaccc tgccctctcc ccggagggga gaggggggag gaggcgggag
     3721 gcggcgcggt aggtgttgcg catgagcatc gcgatggtca tggggccgac gccgcccggg
     3781 accggggtga tggcgcgggc cttcgcggcc a
//
LOCUS       DATMCV010000672         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_635071, whole genome shotgun sequence.
ACCESSION   DATMCV010000672 DATMCV010000000
VERSION     DATMCV010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635071"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VNU03_15410"
     CDS             complement(<1..897)
                     /locus_tag="VNU03_15410"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75909.1"
                     /translation="MERRTFLGVIAGGLLAAPLAVEAPAADKIPLVGILDNGVPRLFT
                     AFREGLHELGYVEGQNVHLVVKSAQGRPDLLPGLVAELVALKPEVVVTTGVAVRAVRR
                     ASPTIPIVVAATRDAVTAGFTSSLAKPADNITGQSFLNTELSAKRLALLKDMLPRARR
                     IAMLVYADSGPDRDVRETRTAAQHLGLHVQRVDVRLDDLARSFSDAKKERADAVDVLA
                     SAWLNAHRERLVELAAQNGLPAIYESRDYVDAGGLMSYGQNITLLFRRAAVYVHKILK
                     GATPADLPWEQPSQFELVINLKT"
     gene            complement(920..>2003)
                     /locus_tag="VNU03_15415"
     CDS             complement(920..>2003)
                     /locus_tag="VNU03_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HWP75910.1"
                     /translation="IRFGQIAAIAPSIPADRAVQRIDAAGKLVTPGLIDLHTHLCPHL
                     GIGLPADELVPITATTTAVSAGDAGAYTFGNFRHGVVPQSRTRLFGFVHIASIGLAGG
                     LAPGEMLNIEYANVEACAKAVAENADLALGVKVRITDSVVGQNGLEPLRRAIRAAEMA
                     GKQFRVMCHIGSAPGSLSDLLDLLRPGDILTHAYSGAGNNTVQNGRVLPAALAAKQRG
                     VIVDVGHGGGSFDFTVCEPAMQQGFGPDTISSDIHAVSINTPGYPTMPWVMSKLLALG
                     MPLEDVVARSTSEPGRIIGKVPGLGTLAIGAPADLAIFDLVDGPVEFVDTRNNKRAGT
                     RKLVPVLTVKGGRPYGRPPLPIPFLY"
BASE COUNT          294 a          690 c          716 g          303 t
ORIGIN      
        1 ggtcttgagg ttgatgacga gctcgaactg cgagggctgc tcccacggca ggtcggcggg
       61 ggtggcgccc ttcagaatct tgtgcacgta tacggccgcc cgccggaaga gcagggtgat
      121 attctgcccg tatgacatga ggccgcccgc gtccacatag tcccggctct cgtagatcgc
      181 gggcagcccg ttctgcgcgg ccaactccac gagccgctcg cggtgcgcat tgagccacgc
      241 ggacgccaga acgtcgaccg cgtcggcccg ctctttcttc gcatcgctga atgagcgcgc
      301 gagatcatcc agccggacat cgacgcgttg cacgtgcaat ccgaggtgtt gcgcggcggt
      361 ccgcgtctcc ctcacgtcgc ggtccggacc ggaatccgca tacacgagca tggcgatccg
      421 gcgggcgcgg ggcaacatgt ccttcaacaa ggcgagccgc ttggcgctca gctcggtgtt
      481 caggaacgac tggcccgtga tgttgtcggc gggcttggcg aggctgctgg tgaagcccgc
      541 ggtgaccgcg tccctggtcg ccgcgacgac gatcgggatc gtcgggctgg cccgccggac
      601 cgcgcgaacc gcaaccccgg tcgtcaccac gacctcgggc ttgagcgcca ccagctcggc
      661 gacgagcccc ggaagcaggt cgggccgccc ctgagcgctc ttcacgacga ggtggacgtt
      721 ttgaccctcg acatacccga gctcgtgcag gccctcgcgg aacgcggtga acaggcgcgg
      781 cacgccattg tccaggatgc ccacgagggg gatcttgtct gccgccggcg cctccaccgc
      841 gagtggcgcg gcgaggagac cgccagcgat cacgccaagg aaggtccgtc gctccactcg
      901 atcacctgat ccgcccgctc tagtacagaa acgggatcgg cagcggcggc cgcccgtagg
      961 gacgcccgcc cttcacggtc agcaccggca ccagcttccg ggtgcctgcg cgcttgttgt
     1021 tgcgcgtgtc cacgaactcc accggcccgt ccaccaggtc gaagatggcc aggtcggccg
     1081 gcgcgccgat cgcgagcgtg ccgagccccg gcaccttgcc gatgatgcgc cccggctccg
     1141 acgtggagcg ggccaccacg tcctcgagcg gcatcccgag ggccaggagc ttggacatga
     1201 cccacggcat ggtcgggtac ccgggggtgt tgatggagac cgcgtggatg tcgctcgaga
     1261 tggtgtcggg gccgaagccc tgctgcatcg cgggctcgca caccgtgaag tcgaagctgc
     1321 cgccgccgtg gcccacgtcg acgatcaccc cgcgctgctt ggcggccagt gcggcgggca
     1381 ggacccgccc gttctgcacc gtgttgttgc cggccccgct gtaggcgtgg gtaaggatgt
     1441 cgcccgggcg cagcaggtcc agcaggtcgg agaggctgcc gggggccgat ccgatgtggc
     1501 acatcacccg gaactgcttg cccgccatct cggcggcgcg gatggcgcgc cggagcggct
     1561 cgaggccgtt ctgccccacc accgagtcgg tgatgcgcac cttgacgccg agcgccaggt
     1621 ccgcgttctc ggccaccgcc ttggcgcagg cctccacgtt ggcgtactcg atgttgagca
     1681 tctcgccggg ggcgagtccg ccggcgaggc cgatggaggc gatgtggacg aagccgaaca
     1741 ggcgggtgcg cgactggggc acgacgccgt ggcggaagtt cccgaaggtg tacgcgcccg
     1801 cgtcgcccgc cgacaccgcg gtggtggtgg cggtgatcgg caccagctcg tcggcgggca
     1861 gcccgatgcc gagatgcggg cagaggtgcg tgtgcaggtc gataaggccc ggggtcacca
     1921 gcttgcccgc cgcgtcgatg cgctgcaccg cccggtcggc cgggatgctc ggcgcgatcg
     1981 ccgcgatctg cccgaagcgg atg
//
LOCUS       DATMCV010000673         3053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_903986, whole genome shotgun sequence.
ACCESSION   DATMCV010000673 DATMCV010000000
VERSION     DATMCV010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903986"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..425
                     /locus_tag="VNU03_15420"
     CDS             240..425
                     /locus_tag="VNU03_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75911.1"
                     /translation="MEPTEDLGEYTTLIVRVRQDDAGRMSGVVERVRTGEKVQFHGLE
                     TLGRALASLFSTGAQGD"
     gene            428..835
                     /locus_tag="VNU03_15425"
     CDS             428..835
                     /locus_tag="VNU03_15425"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWP75912.1"
                     /translation="MTTDTAMTTDVTMTHTLPGPRLKRVTEFIALHLDEDLTLAQLGA
                     VACMSPFHFARLFKRSTGLPPHRFVVRARIDQARALLAEQRRLPIRRIAEVVGFRTVS
                     HFTAVFHRVTGTTPRAYRAAYRPVSTGGHDDVL"
     gene            822..1430
                     /locus_tag="VNU03_15430"
     CDS             822..1430
                     /locus_tag="VNU03_15430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP75913.1"
                     /translation="MTSYEERGLAVADRDATLVAQLRGREAGAAEELVGAYGDRVYRL
                     AIRITGSPSDAEEVTQDALWTASRKIDSFRGAAAFGSWLYRITANAAYQKLRGRRNAR
                     HEISWDDLPPAFDGKGQYLEPVPDWSPKLRDPAIDAELRAALRTAIDDLPGEYRAPLV
                     LHDVEGLSTQEIAEALQVKPGTVKSRVHRARLFLRKRLAELR"
     gene            complement(1427..2965)
                     /locus_tag="VNU03_15435"
     CDS             complement(1427..2965)
                     /locus_tag="VNU03_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013761620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP75914.1"
                     /translation="MRRRIGIDVGGTNTDAVLLEEDTVAAAVKTPTTQDVTTGITRAL
                     ASLVQQAPGARAAQAVMIGTTHFTNAVVQRRDLGRVAAVRIGLPSGASLPPFVDWPAD
                     LADLVRAEVVMLEGGHEFDGRPLVPFDVRGMRAAARRIREAGIRSVAVAAVFSPLNAS
                     GEEEAGAIFQEECPDVAVTLSHRLGRIGLLERENAALLNACLIELARKTTRAFTEALR
                     GSGIAAPLYLTQNDGTVMLADVAEAYPVYSFASGPTNSMRGAAFLSKRDEALVIDVGG
                     TTTDIGSLRHGFPREANSVVEIGGVRTLFRMPDLLSLGLGGGTLVRREPALTIGPASV
                     GYRLTEQALVFGGDVLTVTDVAVAAGLIDLGDRSRVAALPAALVEATLARVRAMIEEG
                     VDRMKTDAGDAPLIAVGGGSFLVPPRLAGVSEVLNVPHQAVANAVGAAIAQVSGEVDQ
                     IFQDLTREEAIARARRAAEDKAVAAGADRTTINVVEVEDLPLAYLPGNSLRTRVRVVG
                     DIAG"
     gene            complement(2962..>3053)
                     /locus_tag="VNU03_15440"
     CDS             complement(2962..>3053)
                     /locus_tag="VNU03_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75915.1"
                     /translation="PPILMTPKGIEHVGPRAFGYDLDFRSVFA"
BASE COUNT          446 a         1061 c         1125 g          421 t
ORIGIN      
        1 gctgggcctg gaagcccccc agtagagaaa gttttacgcg cgccgtgagc ttcactgccc
       61 ctccaggccc ctcggaagga aacggcgttc agtatgccac aggccttccg gcccccgtac
      121 ccggcctatc cggcccattc acgccatttt ccacgctcct gggcatgatc cgggcatgcc
      181 cctccgcgta caaccgttgt gagagacgag ccatgacatt gatgacaggg gagcgaggca
      241 tggagccaac cgaagatctc ggggagtaca cgacgttgat cgtgcgcgtg aggcaggacg
      301 acgcgggccg gatgagtggc gtggtggagc gggtgaggac gggcgagaag gtccagttcc
      361 acggtctgga gacgctgggc cgcgcgctgg cctcgctgtt ctcgaccggc gcccagggag
      421 attgaggatg acgacggaca cggcgatgac gacggacgtg acgatgaccc acaccctgcc
      481 ggggcctcgg ctgaagcggg tgaccgagtt catcgcgctg cacctggatg aggacctcac
      541 gctcgcccag ctcggcgcgg tggcgtgcat gagcccgttc cacttcgcgc gcctcttcaa
      601 gcgcagcacc ggcctgccgc cgcatcgctt cgtggtgcgc gcgcggatcg atcaggcccg
      661 cgcgctgctg gccgagcagc ggcggcttcc catccggcgg atcgcggagg tggtgggatt
      721 ccggaccgtg agccacttca ccgcggtctt ccatcgcgtg accggcacca cgccgcgcgc
      781 ataccgggcc gcgtaccggc ccgtttcgac aggagggcac gatgacgtcc tatgaggagc
      841 ggggactggc ggtggcggat cgggatgcga cgctggtcgc gcagctgcgt gggcgggagg
      901 cgggagcggc ggaggagctg gtgggggcct acggggatcg cgtgtaccgg ctcgcgatcc
      961 gcatcaccgg gagcccgtcc gacgcggagg aggtgacgca ggacgccctc tggaccgcca
     1021 gccgcaagat cgacagcttc cggggggcgg ccgccttcgg ctcctggctc taccggatca
     1081 ccgcgaatgc ggcctatcag aagctgcggg gccggcggaa tgcccggcac gagatctcgt
     1141 gggacgacct gccgcccgcg ttcgacggca agggccagta tctcgagccg gtgcccgact
     1201 ggtcgcccaa gctccgggat ccggcgatcg acgccgagct gcgggcggcg ctccgcacgg
     1261 cgatcgacga tctgccgggg gagtaccgcg cgcccctcgt cctgcacgac gtggaagggc
     1321 tctcgaccca ggagatcgcg gaggcgctgc aggtcaagcc gggcaccgtc aagtcacggg
     1381 tgcaccgggc gcggctcttc ttgcggaagc gcctggccga gctgcgctag cccgcgatgt
     1441 cgccgactac gcgcacgcga gtgcggagag agttgccggg caggtaggcc agcggcaggt
     1501 cctccacctc caccacgttg atggtcgtcc gatcggcgcc ggcggccacc gccttgtcct
     1561 cggccgctcg ccgcgcgcgc gcgatggcct cctcccgtgt gaggtcctgg aagatctggt
     1621 ccacctcgcc gctcacctgg gcgatcgccg cgcccaccgc gttggccacc gcctggtgcg
     1681 gcacgttcag gacctcggag acgccggcca ggcggggtgg aaccaggaac gagccgccgc
     1741 ccaccgcgat caggggcgcg tcacccgcgt cggtcttcat gcggtccacg ccctcctcga
     1801 tcatcgcgcg gacgcgcgcg agggtcgcct ccaccagcgc ggccggcagc gcggccaccc
     1861 gggaccggtc gcccaggtcg atgagcccgg cggccaccgc gacgtcggtg acggtgagga
     1921 cgtcgccgcc gaacacgagc gcctgctcgg tgaggcgata gcccacgctg gccgggccga
     1981 tggtgagggc cggctcccgg cgcacgagag ttccgccccc caggcccagc gagaggagat
     2041 ccggcatccg gaagagcgtg cggaccccgc cgatctcgac cacgctgttg gcctcgcggg
     2101 ggaagccgtg gcggaggctg ccgatgtcgg tggtggtgcc gcccacgtcg atgacgagcg
     2161 cctcgtcgcg cttggacagg aacgccgccc cccgcatcga gttggtgggc ccggacgcga
     2221 aggagtagac cgggtaggcc tccgccacgt cggccagcat gacggtgccg tcgttctggg
     2281 tgaggtagag cggcgccgcg atgccgctgc cgcgcagggc ctccgtgaac gcgcgcgtgg
     2341 tcttccgggc cagctcgatg aggcaggcgt tgagcagggc cgcgttctcg cgctcgagca
     2401 ggccgattcg cccgagccgg tgggagaggg tcaccgccac gtcggggcat tcctcttgaa
     2461 agatggcgcc ggcctcctcc tcgcccgagg cgttgagcgg cgagaagacc gcggccaccg
     2521 ccaccgagcg gatgccggcc tcgcggatcc tccgggccgc cgcgcgcatc cccctgacgt
     2581 cgaaggggac gaggggccgg ccgtcgaact cgtggccgcc ctcgagcatc accacctcgg
     2641 cgcgcaccag gtcggccagg tccgccggcc agtccacgaa gggaggcagc gaggccccgg
     2701 agggcaggcc gatgcgcacc gcggcgacgc gcccgagatc gcgccgctgc accaccgcgt
     2761 tggtgaagtg ggtggtgccg atcatcaccg cctgcgccgc gcgcgcgccg ggcgcctgct
     2821 gcaccaggga cgcgagcgcg cgggtgatgc cggtggtcac gtcctgggtg gtcggcgtct
     2881 tcaccgccgc cgcgaccgtg tcctcctcga ggagcaccgc gtcggtgttg gtgccgccca
     2941 cgtcgatgcc gatgcgccgt ctcatgcgaa caccgagcgg aagtcgagat cgtagccgaa
     3001 ggcgcgcggg ccgacgtgct cgatgccctt cggggtcatg agaattggag gcg
//
LOCUS       DATMCV010000674         2769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_302869, whole genome shotgun sequence.
ACCESSION   DATMCV010000674 DATMCV010000000
VERSION     DATMCV010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302869"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..928
                     /locus_tag="VNU03_15445"
     CDS             68..928
                     /locus_tag="VNU03_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWP75916.1"
                     /translation="MTVDVVVIGHVTLDQTPRGLRPGGAAYYAAITAHRQGMRVGVLT
                     SFGPDFPREALPRDIEVVTVPSTRTTMFRIAPGRLTEGRRVRELAILGRATDLEAEAL
                     PDEWRDVGLGLLCPVAGEVDPALAAAFPDTSLAVLPQGWMRQRGAGGVVSPQAWEDAD
                     LVLPHVQAVVVSHEDIEPFQKEALEWFQRVPVGAVTRGPRGAILFVNGDQYGVEADVA
                     VELDETGAGDVFATAFLIAYNRDGNAWDAAAVAACCAAASVEGEGVAAIPDRAALEAR
                     LASYRRRQVG"
     gene            995..1369
                     /locus_tag="VNU03_15450"
     CDS             995..1369
                     /locus_tag="VNU03_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HWP75917.1"
                     /translation="MADRKIRITVGKVAADAVLDDSETASAVWKALPLSVAGQTWGDE
                     IYFGIPVKAESEDPREVVELGDLAYWPPGSAFCIFYGPTPASRGSEIRPASPVNVFGR
                     VLGDPKVFKAVRSGTTVRVERA"
     gene            1383..2033
                     /gene="fsa"
                     /locus_tag="VNU03_15455"
     CDS             1383..2033
                     /gene="fsa"
                     /locus_tag="VNU03_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HWP75918.1"
                     /translation="MKIFLDSANVNELREGVAMGLVDGCTTNPSLIAKEKRPFRPLVE
                     EICKVVPGDVSLEVVATDFDGMVKEGKELAQVAPNVVVKCPLTKDGLKAVKYLSGEGI
                     RVNQTLCFSAPQALLSAKAGAVYISPFLGRLDDIGHVGMDLVRDICEIYRNYGYKTQV
                     LAASIRNPLHVIDAAKAGAHVATMPFNVLEMLMKHPLTDIGLKKFLDDWNKLGEKI"
     gene            2249..>2769
                     /locus_tag="VNU03_15460"
     CDS             2249..>2769
                     /locus_tag="VNU03_15460"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP75919.1"
                     /translation="MDTERALMARVARGDQAAFTDLAQRYSGKLLAVAGRLLGSRADA
                     EDAVQRAFLQCFAAAAGYDPRWAVSTWLYRILTNVCVDELRRRATRTAHDDRSAAGEL
                     SAGSSNHHPPAAYLDLHRALEQVPREARVLLALRYVDGLSYGELARIRGISINTVKSQ
                     LARGKSLLRAELG"
BASE COUNT          408 a          931 c         1019 g          411 t
ORIGIN      
        1 tgaccatcgg cggcgggacc tccgagatca tgaaggagat catcgccaag cagatggcgc
       61 tctgaccgtg acggtggacg tcgtcgtcat cgggcacgtc acgctcgacc agacgccgcg
      121 cggcctccgc ccgggagggg ccgcctacta cgcggcgatc accgcccacc ggcaggggat
      181 gcgggtgggc gtgctcacct cgttcgggcc cgacttcccg cgggaggcgc tgccccgaga
      241 catcgaggtg gtcaccgtac cctccacgcg gaccacgatg ttccggatcg ccccgggccg
      301 cctcaccgag gggcgccggg tccgcgagct ggcgatcctc gggcgcgcca ccgatctcga
      361 ggcggaggcg ctccccgacg agtggcgaga cgtggggctg ggcctgctct gcccggtcgc
      421 gggcgaggtc gaccccgcgt tggccgccgc cttccccgac acctcgctgg ccgtcctccc
      481 gcagggctgg atgcggcagc ggggcgcggg cggagtcgtc tcgccgcagg cctgggagga
      541 cgccgacctg gtgctgccgc acgtgcaggc ggtcgtggtg agccacgagg acatcgagcc
      601 cttccagaag gaggccctcg agtggttcca gcgcgtgccg gtcggcgcgg tgacccgcgg
      661 gccccgggga gcgatcctct tcgtcaacgg cgaccagtac ggggtcgagg cggacgtcgc
      721 ggtggagctg gacgagaccg gcgcgggcga cgtgttcgcc accgcgttcc ttattgccta
      781 caaccgcgac ggcaacgcgt gggacgcggc cgcggtcgcg gcgtgctgcg cggccgcttc
      841 cgtcgagggc gagggggtcg cggcgatccc ggaccgggcg gccctggagg cgcggctcgc
      901 gtcctatcgt cgccgccagg tcgggtgagc ggcgcggcgc ttgctaccgg gctcgacccg
      961 gggctacact cggtcgtgcg cacgcggcga gtccatggct gaccggaaga tccgcatcac
     1021 ggtgggcaag gtggccgcgg acgccgtcct cgacgattcc gagaccgcct cggcggtctg
     1081 gaaggccctg cctctcagcg tcgccgggca gacctggggc gacgagatct acttcggcat
     1141 cccggtgaag gccgagagcg aggatccccg cgaggtggtg gagctcgggg atctcgccta
     1201 ctggccgccg gggtccgcgt tctgcatctt ctacgggccg acgccggcga gccggggcag
     1261 cgagatccgg cccgcgagcc cggtcaacgt gttcggccgc gtgctggggg atcccaaggt
     1321 gttcaaggcg gtgcgctccg ggaccacggt ccgcgtggag cgcgcctgag acggaggacg
     1381 acatgaagat ctttctggat agcgcgaatg tgaacgagct gcgagagggt gtggccatgg
     1441 gcctggtgga cggctgcacc accaacccct cgctcatcgc caaggagaag cgccccttcc
     1501 ggccgctggt ggaggagatt tgcaaggtgg tgccgggcga cgtgagcctg gaggtggtgg
     1561 ccaccgactt cgacggcatg gtgaaggagg gcaaggagct ggctcaggtg gctcccaacg
     1621 tggtggtgaa gtgcccgctc accaaggacg ggctcaaggc ggtgaagtac ctgagcggcg
     1681 agggcatccg ggtcaaccag acgctctgct tctcggctcc gcaggccctg ctctcggcca
     1741 aggcgggggc ggtctacatc agcccgttcc tgggccgcct ggacgacatc gggcacgtgg
     1801 gcatggacct ggtccgggac atctgcgaga tctaccggaa ctacgggtac aagacccagg
     1861 tgctggccgc ctcgatccgc aacccgctcc acgtcatcga cgcggccaag gcgggggcgc
     1921 acgtggccac catgccgttc aacgtgctcg agatgctgat gaagcacccg ctcaccgaca
     1981 tcggtctcaa gaagttcctc gacgactgga acaagctcgg cgaaaagatc tagcccgaga
     2041 cgattgtccg cggcccggcc gagaaacggt tgtccgccgt ctgggggaga agacggttgt
     2101 ccgcggcctg ggggagtgcg ggggccattt ctgggccccc gcagatgaag agcctgaaga
     2161 tttaaacgat tgaccgcccg aagctcggct ctgttctggt ttccaccctt tctcgcctcc
     2221 cggccgtata tcccggtaag ggcctgagat ggacaccgag cgagcgctga tggccagggt
     2281 ggcgcggggg gaccaggcgg cgttcaccga cctcgcccag cgctatagcg gcaagctgct
     2341 tgcggtggcc gggcggctgc tcggctcccg cgccgatgcc gaggacgcgg tccagcgcgc
     2401 cttcctccag tgcttcgcgg ccgccgccgg ctacgacccg cggtgggcgg tctccacgtg
     2461 gctctatcgc atcctcacca acgtctgcgt ggacgagctg cgccggcggg ctacccggac
     2521 cgcccacgac gatcggtcgg cggcggggga gctctcggcc ggctcgagca atcatcaccc
     2581 gcccgcggcc tatctggatc tgcaccgggc cctcgagcag gtaccgcgcg aggcgcgggt
     2641 gctgctggcc ctgcgctacg tggacgggct ctcctacggc gagctggcgc gcatccgggg
     2701 catctcgatc aacaccgtga agagccagct cgcgcggggc aagtccctgc tgcgggccga
     2761 gctcggcaa
//
LOCUS       DATMCV010000675         3293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_102930, whole genome shotgun sequence.
ACCESSION   DATMCV010000675 DATMCV010000000
VERSION     DATMCV010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102930"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VNU03_15465"
     CDS             <1..180
                     /locus_tag="VNU03_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75920.1"
                     /translation="MKDRFLIGGPDQVIRALKPFVAEYGVTHLICRVFFPGMPHRHIL
                     RELELIAKEVRPAFA"
     gene            212..1186
                     /locus_tag="VNU03_15470"
     CDS             212..1186
                     /locus_tag="VNU03_15470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP75921.1"
                     /translation="MRWLIVLPFARPGHMGMDFRDELVAMGHEARTFAYRRDNPLYKN
                     RSTKATYQLFILRRLERACVAYRPDFVLVIKGGPVTPGLVRRVKARIDTRFLNFFPDN
                     PLWMIPFECIEAYDAFFTKERYALRALEQAGLRNLHYLPMYCVPDMHHPIELTPEETR
                     RYAAPLSFVGSAYAYRERFVRELAGTPLRLWGAGWDRVEAPEIRAMVAGGPVWGRAKL
                     AVYSGSTLSLNHHHPMNDIVGVNTRAFELAASAACQLVDAKEELAHLFTPGQEAVVYQ
                     DLGELRRHLDYYLAHPDEARDIGERARKRALAEHTLRHRIEEMLAILR"
     gene            1183..2415
                     /locus_tag="VNU03_15475"
     CDS             1183..2415
                     /locus_tag="VNU03_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWP75922.1"
                     /translation="MTAASFATALRCIECGAEFALDYRLECAACRGLLELDYDWEALR
                     RAGPAVLEGRGLWRYAPVLPIADPAHRVSLGEGESPLLDCPTLGRALGVRRLQVKFEG
                     SNPTGTVKDRSSPTAVAAALQFGFRITSVVSSGNAGSSIAAYSARAGLRSLIFAYART
                     SAPKLLHMAATATDLILYEGVYDDLIVLWDRLVEDQLFFDCGASRNAYKHEGKKTLAY
                     EIAEQSGWRVPDVVVAPVAVGETFIATHRGFVEMERLGWIPRRPRMVAAQATRADAIA
                     RAWREGRPLAPVKIGYTVAEGLAAGNPGRKGEWVLRILKETGGLAGDAEDEEILETQG
                     LLARTEGIWAGPTGVAALAVLRKLLAARALDPDATICVILSETGLKTEASGPGRRATA
                     FDEASLRRLVHSLLGGST"
     gene            2412..>3293
                     /locus_tag="VNU03_15480"
     CDS             2412..>3293
                     /locus_tag="VNU03_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWP75923.1"
                     /translation="MKRLLTAVLLALVIAPAAPPAAAQGLVATFKRVNPSVVIIRARW
                     KEVGSSGQMGGVNEVGSGVLISPDGQVITAAHVVQTADEITAEFLSGEVVNARVIASE
                     PEADVSLLQLDRVPAKALVARFGNSDRAEVGDQIFIIGAPYGISHTLSVGYVTGRRKP
                     NTVYSEMALAEFFQTDAAINPGNSGGPMFNMAGEVIGIASHNITKSGGSEGLGFVVTI
                     NMARRLLLEQRSFWSGMSGVIVGGQVARALNLPQPVGLMVQQVAANSPAAHMDLRPGV
                     IPASIEGKSFLLGGDIIL"
BASE COUNT          454 a         1214 c         1131 g          494 t
ORIGIN      
        1 atgaaggacc gcttcctcat cgggggcccc gatcaggtca tccgcgcgct caagccgttc
       61 gtggccgagt acggcgtaac ccacctgatc tgccgcgtct tcttcccggg catgccgcat
      121 cggcacatcc tgcgcgagct cgagctgatc gcgaaggagg tccgtcccgc cttcgcctga
      181 gcgccggccg cggtgtgata cgctttccgc cgtgcgctgg ctgatcgtgc tgcccttcgc
      241 ccgtcccggg cacatgggca tggacttccg cgacgagctg gtggccatgg gccacgaggc
      301 gcggaccttc gcctaccgcc gcgacaaccc gctctacaag aatcgctcga ccaaggccac
      361 ctaccagctc ttcatcctgc gccggctcga gcgggcgtgc gtggcctacc gaccggactt
      421 cgtgctggtg atcaagggcg gcccggtcac ccccggactg gtgcgccggg tgaaggcgcg
      481 catcgacacc cgcttcctca acttcttccc cgacaatccg ctgtggatga tcccgttcga
      541 gtgcatcgag gcctacgacg ccttcttcac caaggagcgc tacgcgctcc gcgcgctcga
      601 gcaggccggc ctgcgcaacc tgcactacct gccgatgtac tgcgtgcccg acatgcacca
      661 cccgatcgag ctcacgccgg aggagacgcg gcgctacgcg gcgccgctct ccttcgtggg
      721 cagcgcctac gcctaccggg agcgcttcgt gcgggagctc gccggcacgc cgctgcgcct
      781 ctggggcgcg ggctgggacc gggtggaggc gcccgagatc cgcgcgatgg tcgcgggagg
      841 tccggtgtgg ggccgcgcca agctcgcggt gtactccggc tccacgctct ccctgaacca
      901 ccaccatccg atgaacgaca tcgtgggggt caacacccgg gccttcgagc tcgccgccag
      961 cgcggcgtgc cagctggtgg acgcgaagga ggagctcgcc cacctcttca cgcccggcca
     1021 ggaggcggtg gtctaccagg atctgggcga gctgcgccgc cacctcgact actacctcgc
     1081 gcacccggac gaggcccgcg acatcggcga gcgcgcgcgc aagcgggccc tcgccgagca
     1141 caccctgcgc caccgcatcg aggagatgct ggcgatcctg cggtgaccgc cgcctcgttc
     1201 gcgaccgcgc tgcggtgcat cgagtgcggc gcggagttcg cgctcgacta ccggctcgag
     1261 tgcgcggcct gccggggcct cctcgagctc gactacgact gggaggccct ccgccgcgcg
     1321 gggcccgcgg tcctcgaggg ccgcgggctc tggcgctacg cgccggtgct gccgatcgcc
     1381 gatccggccc atcgggtctc gctcggagag ggagagagcc cgctgctcga ctgcccgacc
     1441 ctcggccgcg ccctcggggt gcggcggctc caggtgaagt tcgagggctc gaaccccacc
     1501 ggcaccgtga aggatcgctc ctcccccacc gcggtggcgg ccgcgctcca gttcggcttc
     1561 cgtatcacct cggtggtcag ctccggcaac gcgggctcgt cgatcgcggc ctactccgcg
     1621 cgcgccggcc tgcgctcgct gatcttcgcg tacgcccgca cctccgcgcc caagctcctg
     1681 cacatggccg cgaccgccac tgacctgatc ctctacgagg gtgtgtacga cgacctgatc
     1741 gtcctctggg atcgtctcgt cgaggaccag ctcttcttcg actgcggcgc ctcccgcaac
     1801 gcctacaagc acgagggcaa gaagacgctc gcctacgaga tcgccgagca gtcggggtgg
     1861 cgggtcccgg acgtggtggt ggccccggtc gcggtgggcg agacgttcat cgcgacccac
     1921 cgcggcttcg tggagatgga gcgcctcggc tggatcccgc ggcggccccg catggtcgcc
     1981 gcgcaggcga cccgggccga cgcgatcgcg cgcgcgtggc gcgagggccg tccgctcgcc
     2041 ccggtgaaga tcggctacac ggtggccgag gggctcgcgg ccggcaaccc ggggcgcaag
     2101 ggcgagtggg tcctccgcat cctgaaggag acgggtgggc tggccggcga cgcggaggac
     2161 gaggagatcc tcgagacgca gggcctgctc gcgcggaccg aggggatctg ggccggcccg
     2221 accggggtgg cggccctggc ggtgctgcgg aagctcctgg ccgcccgggc cctcgacccc
     2281 gacgccacca tctgcgtgat cctgagcgag accgggctca agaccgaggc gagcgggccc
     2341 ggccgtcggg ccaccgcctt cgacgaggcc tcgctccggc ggctcgtcca ctccctgctc
     2401 ggaggctcga catgaagcga ttgctcaccg ccgtcctgct cgccctggtc atcgcccccg
     2461 ccgccccgcc tgctgcggcc cagggtctgg tggcgacctt caagcgcgtc aacccgtcgg
     2521 tggtcatcat ccgggcgcgc tggaaggagg tgggcagctc cggccagatg gggggcgtca
     2581 acgaggtcgg ttcgggcgtc ctgatctcgc cggacggcca ggtgatcacc gcggcccacg
     2641 tggtgcagac cgcggacgag atcaccgccg agttcctgag cggcgaggtg gtgaacgcgc
     2701 gggtgatcgc ctccgagccg gaggccgacg tctccctgct ccagctggac cgcgtccccg
     2761 cgaaggcgct ggtggcccgg ttcggcaact cggatcgggc cgaggtcgga gaccagatct
     2821 tcatcatcgg cgccccctac ggcatctccc acaccctgag cgtgggctac gttaccggtc
     2881 gccgcaagcc caacaccgtc tacagcgaga tggcgctcgc cgagttcttc cagaccgacg
     2941 ccgccatcaa tcccggcaac tcgggcggcc ccatgttcaa catggcgggc gaggtcatcg
     3001 ggatcgcgag ccacaacatc accaagtcgg gtgggtccga gggcctgggc ttcgtggtca
     3061 cgatcaacat ggcccgacgc ctcctgctcg aacagcgctc gttctggagc ggcatgagcg
     3121 gggtgatcgt cggcgggcag gtcgcccgcg cgctcaacct gccccagccg gtcgggctga
     3181 tggtgcagca ggtcgcggcg aactcccccg cggctcacat ggatctccgg cccggggtca
     3241 tccccgcctc gatcgagggc aagtcgttcc tgctgggcgg cgacatcatc ctc
//
LOCUS       DATMCV010000676         4201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_302999, whole genome shotgun sequence.
ACCESSION   DATMCV010000676 DATMCV010000000
VERSION     DATMCV010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302999"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VNU03_15485"
     CDS             <1..903
                     /locus_tag="VNU03_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75924.1"
                     /translation="TRFLTTRTPFRHVPRLAGWVEYESADGLADLAVLQEFVANRGDG
                     WKHVVTTLREGLAAGAGRLASDPLIEDMRRLGAITGGLHAALASDGADPDFAPEPIDR
                     EDVARWAADIRRDVEAADVLAPADTSRDGVERTLSSLEALVGTVKIRSHGDYHLGQVL
                     KTEDGFAVIDFEGEPARPLAERRARQSALRDVAGMLRSLDYAAHAVAFEVPAPERAAA
                     LAALTAWEARARRAFLEGYRGAAGASPVALIPPSEGALIAACAAFELEKACYELRYER
                     DNRPDWVAIPLAGIRRILLTPGRA"
     gene            complement(884..1093)
                     /locus_tag="VNU03_15490"
     CDS             complement(884..1093)
                     /locus_tag="VNU03_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75925.1"
                     /translation="MIGRSEDLLGECRAADSGLAAIVESVLCEEGPYKVVAAAGVEAW
                     REREPNLWRKVAEWLDERGVTLVPV"
     gene            1364..1513
                     /locus_tag="VNU03_15495"
     CDS             1364..1513
                     /locus_tag="VNU03_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HWP75926.1"
                     /translation="MLWTIFVILLVLWALGMVSSYTLGGFIHLLLLIAIAVVLIRIIQ
                     GRRPV"
     gene            1545..2147
                     /locus_tag="VNU03_15500"
     CDS             1545..2147
                     /locus_tag="VNU03_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HWP75927.1"
                     /translation="MNYKVQEHLKPKALAGISDDQIAQHWALYEGYVKNANGLLEDTA
                     KAEAGSPAWAELRRRLGFEMNGLVLHEYYFANLASGSKLSPGSDLAADVGAAWGSVGA
                     WREDFTKTGGIRGVGWAILYHDPATGTLLNCWISSHEQGHPAGFHPVLVLDVFEHAFM
                     VDYGAGGRADYIAAFLDNVNWEVVEQRHKDSKAGRYTARF"
     gene            2157..2378
                     /locus_tag="VNU03_15505"
     CDS             2157..2378
                     /locus_tag="VNU03_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 domain-containing protein"
                     /protein_id="HWP75928.1"
                     /translation="MKSGVLVVAIVLIAIGVVSLAYQGITYTTREKVLEVGPIKATKD
                     KEHTLPLPPILGGLALAGGVVLLVAGARR"
     gene            2518..3174
                     /locus_tag="VNU03_15510"
     CDS             2518..3174
                     /locus_tag="VNU03_15510"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWP75929.1"
                     /translation="MSLPAPKLPREFYAHSSVVTVARRLLGQLLVVPTRSGRVSGIIV
                     ETEAYRGPEDRASHAWGGRRTLRTETMYARGGTAYVYFVYGMYFQFNVVTGVADVPHA
                     VLVRAVEPVDGVALMRRRRGVGTLHGLTSGPGKLCIALGIDRRLDRADLLGDRIWIEA
                     RPPVPARAIAAGPRVGIDYAETWVDRPWRFWLRDNPFVSRSPAGVRRGGGARSGGAPT
                     "
     gene            complement(3131..>4201)
                     /locus_tag="VNU03_15515"
     CDS             complement(3131..>4201)
                     /locus_tag="VNU03_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HWP75930.1"
                     /translation="LTVAAMVLVNNPGSWASVYWPLRHAEWSGFTPTDMIFPLFLFIV
                     GVSIPLALGPRLEERRAAGLVGRVLWRSLAIFTLGLLLHALPWFHLATLRIPGVLQRI
                     AVCYLLAALLVIASGGGAGWRLQAVVAGGLLVGYWLLMTRVAPPGGVAGDLSPAGNLA
                     GYVDRVVLGPHLWQVAKFYDPEGVLSTLPAVATTLLGVLAGHWIRGAGPTGRTVGGLV
                     IGGVAATAAGWLWAQSFPINKSLWTSSYALFMSGLAALALAACYWMIEVRGWRRVTGP
                     FVVLGVTALPLFFLSSLMARLLLLIRVGPDRIRLHAWLFEHLFAPWASPFNASLAFAL
                     AYVLLWWGLMWALHRSGLRLRV"
BASE COUNT          638 a         1532 c         1467 g          564 t
ORIGIN      
        1 acgcgcttcc tcacgacccg caccccgttc cgtcacgtcc cgcgcctggc cggctgggtc
       61 gagtacgaga gcgccgacgg gctcgccgac ctcgcggtgc tccaggagtt cgtggcgaac
      121 cggggcgacg ggtggaagca cgtcgtgacg acgctccgcg aaggtctcgc ggccggggcc
      181 ggtcgcctcg cttccgatcc cctgatcgag gacatgcggc gtctcggtgc gatcaccggg
      241 ggcctccacg ccgcgctcgc gtcggacggg gcggacccgg acttcgcgcc ggagccgatc
      301 gaccgcgagg acgtcgcgcg ctgggccgcc gacatccgcc gcgacgtgga ggccgccgac
      361 gtcctcgcgc ccgccgacac gagccgggac ggcgtcgagc ggacgctctc atcactcgag
      421 gcgctcgtcg gcaccgtgaa gatccggagc cacggcgact atcacctggg ccaggtgctg
      481 aagaccgagg atggcttcgc ggtgatcgac ttcgagggcg agcccgcgcg ccccctggcg
      541 gagcgccggg cccggcagtc ggccctgcgt gacgtggccg gcatgctgcg ctcgctcgac
      601 tacgcggcgc acgcggtggc cttcgaggtg cccgcgcccg agcgcgcggc ggccctggcc
      661 gcgctcaccg cgtgggaagc tcgggcccgc cgcgccttcc tcgagggcta ccggggcgcc
      721 gccggcgcga gcccggtggc gctgattcct ccgtcggagg gcgcgctgat cgcggcctgc
      781 gccgccttcg agctcgagaa ggcctgctac gagctgcgct acgagcggga caaccgaccg
      841 gactgggtgg ccatcccgct ggccggcatt cggcgcatcc tgctcacacc gggacgagcg
      901 tgaccccgcg ctcgtccagc cactccgcca cttttctcca gagattgggc tcccgctcgc
      961 gccaggcctc cacgcccgcc gcggccacga ccttgtaggg accttcctcg cacagcacgg
     1021 actccacgat cgcggccaga ccgctgtcgg cggcccggca ttctcccagc aggtcctcgc
     1081 ttcgaccgat catgggccgc gggaatcgca aggcccgtgc caccggctcc gccgcaccgt
     1141 atcacgggta tccggtcttc gagccccgga tcgaggtgct tctaccgagt agtcctgact
     1201 ggatcgcgat catccgcgtg tccgctgacg cgggatacgg cggcatctgc cgggcttaca
     1261 ggcagtttgc cagcatcgca ctcgctggca tgtcgcgtgc gtatacagcc cgccgaacgt
     1321 acgaactcga cactcacagc tcgatacccg aggagggcaa ctcatgttgt ggacgatctt
     1381 cgtgatcctg ctggtgctgt gggcgctggg catggtcagc tcgtataccc ttggcggctt
     1441 catccatctg ctgctcctca tcgccatcgc ggtggtcctg atccgcatca tccaggggcg
     1501 ccgacccgta tagggggcgc cacccccatg aactacccat cgccatgaac tacaaagttc
     1561 aggagcacct caagcccaag gcgctggccg gcatctcgga cgaccagatc gcgcagcact
     1621 gggcgctcta cgaaggctac gtgaagaacg ccaacggcct cctggaggac accgcgaaag
     1681 cggaggccgg ctcgcccgcg tgggccgagc tgaggcggcg gctcggcttc gagatgaacg
     1741 gactggtgct gcacgagtac tacttcgcca acctcgcgtc gggctcgaag ctgtcccccg
     1801 gaagcgatct ggccgcggac gtgggcgcgg cgtggggctc ggtcggcgcg tggcgggagg
     1861 acttcaccaa gaccggcggc atccgcggcg tgggctgggc gattctctac cacgatcccg
     1921 ccaccgggac cctgctcaac tgctggatct cgagccacga gcagggtcat ccggccgggt
     1981 tccacccggt cctggtcctg gacgtcttcg agcacgcgtt catggtggac tacggcgcgg
     2041 gcgggcgggc cgactacatc gcggccttcc tcgacaacgt gaactgggaa gtcgtcgagc
     2101 agcgccacaa ggacagcaaa gccgggcgct acaccgcccg cttctgagag gagcccatga
     2161 aaagcggagt cctggtcgtc gccattgtgc tgatcgccat cggtgtcgtc tcgctcgcct
     2221 accagggcat cacctacacg acccgggaga aggtcctcga ggtcgggccc atcaaggcga
     2281 cgaaggacaa ggagcacacc cttccgctgc cgccgatcct gggcgggctg gccctggccg
     2341 gcggcgtggt cctgctcgtg gccggggcgc gccgctagcg cgagcctccg gcatccccgt
     2401 caccggagcc gggcgcccca agcgccccat ccggtgcgag ccgcgggcgc ggtcccttct
     2461 cggccgcgcc cgcggtgttt cgcccgcccg gtggctttcg tggcacactg gcgggccgtg
     2521 agcctccccg ctccgaagct gccgcgcgag ttctacgcgc actcgagcgt cgtcaccgtc
     2581 gcccgccgac tcctcggcca gctgctggtg gtgccgaccc ggtccggccg ggtctcgggc
     2641 atcatcgtgg agaccgaggc ctaccgtggc cccgaggatc gcgcctcgca cgcgtggggc
     2701 ggccgccgga cgctccgcac cgagaccatg tacgcccgcg gcggcaccgc ctacgtctac
     2761 ttcgtctacg gcatgtactt ccagttcaac gtggtgaccg gggtggccga cgttccccac
     2821 gcggtcctcg tgcgggcggt ggagccggtc gacggcgtgg ccctgatgcg gcggcggcgc
     2881 ggcgtcggga cgctccacgg gcttaccagc ggcccgggca agctctgcat cgcgctcggc
     2941 atcgaccggc ggctcgaccg ggccgacctg ctcggcgacc ggatctggat cgaggcgcgc
     3001 ccgccggtgc cggcgcgggc catcgcggcg ggaccgcgcg tgggtatcga ctacgcggag
     3061 acctgggtgg accgcccgtg gcgcttctgg ctccgggaca accccttcgt gagccggtcg
     3121 ccggccggcg tcagacgcgg aggcggagcc cgctccggtg gagcgcccac atgaggcccc
     3181 accagagcag cacgtacgcc agggcgaagg ccagggacgc gttgaacggc gaagcccacg
     3241 gcgcgaagag gtgctcgaag agccaggcgt gcaggcggat gcggtcgggc ccgacccgga
     3301 tcagcaggag caggcgcgcc atgaggctcg acaggaagaa gagcggcagc gcagtgacgc
     3361 ccagcaccac gaagggtccc gtgacgcgcc gccagccccg cacctcgatc atccagtagc
     3421 aggcggcgag cgcgagcgcc gcgaggccgc tcatgaacag cgcgtacgag ctggtccaca
     3481 gcgacttatt gatcggaaaa gactgcgccc acagccagcc ggccgccgtc gcggcgacgc
     3541 caccgatcac gagcccaccg accgtgcggc ctgtcggccc cgccccacgg atccagtgcc
     3601 cggcgaggac gccgaggagc gtggtcgcca ccgcgggaag cgtgctcaag acgccctccg
     3661 ggtcgtagaa cttcgccacc tgccagaggt gcggcccgag caccacgcgg tcgacgtagc
     3721 cggccaggtt gcccgccggg ctcaggtccc cggccacccc gcccggagga gccacgcgcg
     3781 tcatgagtag ccagtagccg acgagcagcc cgcctgcgac gacggcctgc agacgccagc
     3841 ccgcgccgcc gccgctcgcg atcaccagca gcgcggccag caggtagcag accgcgatcc
     3901 gctggagcac gccggggatg cggagggtgg cgagatggaa ccacggcagc gcgtgcagca
     3961 ggagaccgag ggtgaagatc gcgagggatc gccacagcac gcggcccacc agccccgcgg
     4021 cccgccgctc ctccagccgg ggcccgaggg ccagcgggat cgacacgccg acgatgaaga
     4081 ggaagagcgg gaagatcatg tccgtcgggg tgaacccgct ccactccgcg tgccggaggg
     4141 gccagtagac ggacgcccac gagccgggat tgttcaccag caccatcgcg gccaccgtga
     4201 g
//
LOCUS       DATMCV010000677         6974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_569562, whole genome shotgun sequence.
ACCESSION   DATMCV010000677 DATMCV010000000
VERSION     DATMCV010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569562"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1393)
                     /locus_tag="VNU03_15520"
     CDS             complement(191..1393)
                     /locus_tag="VNU03_15520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75931.1"
                     /translation="MESWERNVWALALVVFIAFVGFQFFSPFLPLYVMELGVTDPTRV
                     ALWSGVLTAVTPAVSGLLGPLWGSFADRVGRKMMMVRSLVGFVIIVAAMGLVTSVTQL
                     LILRVLQGVIAGFSIFAMALASVSCPKERVPVAIGRVQGAQLLSVAIGPAAGGYVASH
                     FGLRYAFFVTAGMCALALVGLLVLFTENRPLGNAGPAAATSDTGFTLRQVWTSRGFPL
                     VLVLLFVAQFIDRGLSLLVPLKVGTLLDVTRIAATSGEIISLAAICATGSALLAGRLA
                     QRWPAERLLLIGLVAGGLPCAFMAFTTGWQSLMLFRCLTGLFLGGALTLAYSIGGSLV
                     TGERRGAAFGWLALSVQIGTAASPLVSGGLAAISLPGAFVLDGTLAWLAAGVLLLGAR
                     HLRRGAAP"
     gene            complement(1413..2480)
                     /locus_tag="VNU03_15525"
     CDS             complement(1413..2480)
                     /locus_tag="VNU03_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75932.1"
                     /translation="MRKAAIVLAVLVLWAPAAALAQTTKLRVAICARTISMGVGSPFA
                     VAMKMGWFKQEGLDIEIVPLPGSTDCVKAVATGEVPISLPSVEPLANGRPQGVKAKIY
                     YTAYQTNGYGIAVPADSPIQSPSELRGKSIGVTSLASAGVVVARAQVAAAGLDPARDV
                     QIVVAGEGAQTAAMVRNKQVEALSQFDTQYAMVENAGVRMRYLDRRDIEKFPGNGFLA
                     SEETLRTRRKELIGMARAYAKGTIFTIANPEAAVHIVYEMFPQTKPTGKDEVTAVRED
                     VKVLEARMPHYKLEPAGVKRWGENSEVNYRDYVDFLVKWSVITQKVPTSDLITNDLIE
                     DINRMDAGKIAAEAAAYRYSR"
     gene            complement(2504..3370)
                     /locus_tag="VNU03_15530"
     CDS             complement(2504..3370)
                     /locus_tag="VNU03_15530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP75933.1"
                     /translation="MIERRPFGRTGHQSTVTLFGAAALARASQDEADRALEVLLRHRV
                     NHIDTAARYGDSELRIGPWMARHRKDFFLATKTGSRGGREAREDLHRSLERLRVDSVD
                     LIQMHSLVHPDDWDQAMGPGGALEALVEAKQQGLVRAIGVTGHGWTIAAMHRRSLARF
                     DFDSILLPYTFFMMQNERYRQAFEEVATICRERGAAVQTIKAIARGPWAAADRSHATW
                     YQPLERQEDIDRAVHWVVGRPGVFLNTAGDLGLLPKVLDAASRFTRRPSDEEMTALHD
                     ATRMSSLFGLPT"
     gene            complement(3398..5722)
                     /locus_tag="VNU03_15535"
     CDS             complement(3398..5722)
                     /locus_tag="VNU03_15535"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWP75934.1"
                     /translation="MLVGASPKRKEDGRFVAGGGRYLDDVRVAGLLHLALVRSPHAHA
                     RVRAIDAAAARAVPGVVAVLTLDDLPELADATVPPLVPEPKGRPYVHPVLAGARVRHV
                     GEPVAVVVASDPYTAADGVERVVVAYDPLPAATSPAAAIAPGAPRVNETWPDNLASVT
                     ASAKGQPEEALARAPVVVAARLAYPRVAGMPLETRGVLAAPDPIGGGLTVWTSTQVPF
                     AVRSAIAPVVRLAEERVRVLVPDVGGGFGVKGHVYPEEILVAVVARRLGRPVKWAETR
                     SEHCLTAAGDRDQVHEARIGLERDGRIIAIETAFTRDHGVAPTLGEAITLNTINHLPG
                     PYRVPHYRGTGQNVLTHKTFAAAYRGAGRPEAAFVLDRLLDRAAREIGMDPAALRRLN
                     LIRPDEMPVPTGLTYRDGAPIVYDPADYPAAFDRLLATLDYPGWRARQAERRGGPRPI
                     GIGLCAYVEGTGIGPFEGADIRVDPDGTVFVHLGVCAQGQGHETTLAQIAADELGVAL
                     ESVAVVGGDTSLVGYGMGTIASRVAAVGGPAVQRSAAQVAHKARLVGAEMFECAPEDV
                     VLAEGRVGVRGVPGKSVSLGQVARAAVRSRAVAEAGGPGLSACAFFYPDTVTWAFGVQ
                     GIVLEVDLEACAITLLRLAAMHDCGRPINPVIVEGQLHGGIAQGLGSALGEELIHDDA
                     GQLLTGTLMDYPMPRADDMPPLDVVHMNFPSAINPLGIKGVGESGVISPAAAVANAVE
                     DALADYGVLVDRPPVTSARVFELLRATGRWPAGR"
     gene            complement(5745..6266)
                     /locus_tag="VNU03_15540"
     CDS             complement(5745..6266)
                     /locus_tag="VNU03_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP75935.1"
                     /translation="MTQTAQPAQSLDETLRTHVARFTERRADWDAFADARVEGFKRAQ
                     HRFIGSGASGKTDASVIPAQHFTLSVMFIPPGQGNAPHTHEVEEVFFILSGKVLVFIE
                     DGAGHRAETVLGPWDCVSCPANVIHGFTNVGLEPAYLQVMLGKARPELMTYTDGDLQS
                     RRDEHLRAAGARG"
     gene            6413..>6974
                     /locus_tag="VNU03_15545"
     CDS             6413..>6974
                     /locus_tag="VNU03_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75936.1"
                     /translation="MGKIVFAGAMSHVLDPDYYQRACGDEGRRMVTDCMTEIARMGER
                     FSATGADALIVVADDHLNAFSFNAVPALCVRIGRTVQRMKQDHAEAFDAVLDHMPDEY
                     PLHEDLANQILEKGLGSGFDLAMSWEAPVDHAFLSPVNTMHGTRPIPPLVPIWVNCFV
                     APQPTPKRCFDFGRHIARVVAASPWNV"
BASE COUNT          965 a         2482 c         2588 g          939 t
ORIGIN      
        1 ccgcccgagc cctcgtgggg caccgacgtc gggcgcggct accggttcat gcggctggcc
       61 ccgtggctgg tcttcatgcc ggggaccgca attctattga ccgcgctcgc gttcaacctg
      121 gtgggcgacg gcatccgcga cgccttcgat ccgcgccaga gccgggaggg ctagcgcgcc
      181 gtcgcggtcc tcagggggcc gccccccgtc gcaggtggcg cgcccccagc agcaagacgc
      241 cggcggccag ccacgcgagc gtgccgtcca gcacgaaggc gccgggcagg ctgatcgcgg
      301 cgagcccccc gctcaccagc gggctcgccg cggtgccgat ctgcacgctg agcgcgagcc
      361 agccgaaggc ggccccgcgg cgctcaccgg tcacgaggct gccgccgatc gagtaggcga
      421 gcgtcagtgc gccgcccagg aagagcccgg tgaggcagcg gaagagcatc aggctctgcc
      481 atcccgtcgt gaaggccatg aaggcgcagg gcagcccacc cgcgaccagg ccgatcagga
      541 gcaggcgctc cgccggccag cgctgggcga gccggcccgc gaggagagcc gagccggtgg
      601 cgcagatggc ggcgagcgag atgatctcgc ccgaggtcgc ggcgatgcgg gtgacgtcga
      661 gcagggtccc gaccttgagc gggacgagca gcgagagccc gcggtcgatg aactgggcga
      721 cgaacaggag gaccagtacc agcgggaacc cgcgcgaggt ccacacttgg cggagggtga
      781 agccggtgtc agacgtggcc gcggcgggcc ccgcgttgcc caggggccgg ttctcggtga
      841 agagcaccag caggccgacg agggcgagcg cgcacatgcc ggcggtgacg aagaacgcgt
      901 agcggagacc gaagtgggag gccacgtagc cgcccgcggc gggcccgatg gccacgctca
      961 ggagctgcgc gccctgcacg cgcccgatgg ccaccggcac gcgctccttg gggcacgaca
     1021 cgctggccag ggccatcgcg aagatggaga agccggcgat cacgccctgg agcacgcgca
     1081 ggatcaggag ctgggtgacc gaggtgacga gccccatcgc ggccacgatg atcacgaagc
     1141 ccaccagcga gcggaccatc atcatcttgc ggccgacccg gtccgcgaag ctgccccaga
     1201 gcgggcccag cagccccgag accgcggggg tcaccgcggt gagcaccccc gaccagaggg
     1261 cgacccgggt ggggtcggtg acccccagct ccatcacata gagcggcagg aagggcgaga
     1321 agaactggaa gccgacgaac gcgatgaaga cgacgagggc cagcgcccag acgttgcgtt
     1381 cccagctctc cactcggccc gggcgtcccc gtctaccggc tgtaccggta ggccgccgcc
     1441 tccgcggcga tcttgcccgc gtccatccgg ttgatgtcct cgatgaggtc gttggtgatc
     1501 aggtcggagg tcggcacctt ctgggtgatc acgctccact tcacgaggaa gtccacgtag
     1561 tcgcggtagt tgacctcgga gttctcgccc cagcgcttca cccccgccgg ctcgagcttg
     1621 tagtggggca tgcgcgcctc gagcaccttg acgtcctcgc gcaccgcggt gacctcgtcc
     1681 ttgccggtgg gcttggtctg cgggaacatc tcgtagacga tgtgcaccgc ggcctcgggg
     1741 ttggcgatgg tgaagatcgt ccccttggcg taggcgcgcg ccatgccgat cagctccttg
     1801 cgtcgcgtcc ggagcgtctc ctcggacgcg aggaagccgt tgccggggaa cttctcgatg
     1861 tcgcgccggt cgaggtagcg catgcggacg cccgcgttct ccaccatcgc gtactgggtg
     1921 tcgaactggc tgagcgcctc cacctgcttg ttgcgcacca tcgccgcggt ctgcgcgccc
     1981 tcgcccgcca ccacgatctg cacgtcgcgc gcggggtcga ggccggccgc cgccacctgg
     2041 gcccgcgcca ccaccacgcc ggcggaggcg aggctggtga ccccgatgga cttgccgcgc
     2101 agctcgctcg ggctctggat cgggctgtcc gcgggcaccg cgatgccgta gccgttggtc
     2161 tggtacgcag tgtagtagat cttggccttg actccctgcg gccggccgtt ggcgaggggc
     2221 tccacgctcg ggagcgagat cggcacctcg ccggtggcca ccgccttcac gcagtcggtg
     2281 gagccgggca gcgggacgat ctcgatgtcg agcccctcct gcttgaacca gcccatcttc
     2341 atggccaccg cgaacgggga accgacgccc atgctgatag tccgggcgca gatggccacc
     2401 cgcagcttgg tggtctgggc gagcgccgcg gcgggcgccc acaggacgag cacggcgagg
     2461 acgatggcgg ctttgcgcat ggagcgttgg tctcctctcg gggtcaggtc ggcagtccga
     2521 acagtgacga catccgggtg gcgtcgtgca gggccgtcat ctcctcgtcg gagggacggc
     2581 gggtgaagcg gctcgccgcg tcgaggacct tcgggagcag gccgaggtcg cccgcggtat
     2641 tcaggaagac gccgggccgg cccaccaccc agtgcaccgc gcggtcgatg tcctcctggc
     2701 gctcgagggg ctggtaccag gtggcgtggc tccggtcggc ggcggcccac ggcccgcgcg
     2761 cgatcgcctt gatggtctgc accgcggcgc cgcgctcgcg gcagatggtg gcgacctcct
     2821 cgaaggcttg ccggtagcgc tcgttctgca tcatgaagaa ggtgtagggc agcaggatcg
     2881 aatcgaagtc gaagcgcgcg aggctccgcc ggtgcatggc cgcgatggtc cagccgtggc
     2941 cggtcacccc gatggcccgc acgagcccct gctgcttggc ctcgacgagg gcttcgagcg
     3001 cgccgcccgg gcccatggcc tggtcccagt cgtcggggtg gacgagcgag tgcatctgga
     3061 tcaggtcgac cgagtcgacg cgcagccgct cgagcgagcg gtggagatcc tcgcgcgcct
     3121 cgcgcccgcc gcgcgagccg gtcttggtgg cgaggaagaa gtccttgcgg tggcgcgcca
     3181 tccacggccc gatgcgcagc tcggagtcgc cgtagcgcgc ggcggtgtcg atgtggttga
     3241 cccggtgccg cagcagcacc tcgagcgcgc ggtcggcctc gtcctggctg gcgcgggcca
     3301 gcgcggccgc gccgaacagg gtgacggtgg actggtggcc ggtgcggccg aacggtcgac
     3361 gctcgatcat cgtgtcctcc tccttgtcag ctaggtctca gcggccggcg ggccagcggc
     3421 cggtcgcgcg cagcagctcg aacacgcggg ccgaggtgac cggcggccgg tccaccaaga
     3481 cgccgtagtc cgcgagggcg tcctccaccg cgttggccac cgcggcggcc ggcgagatga
     3541 cgccgctctc gcccaccccc ttgatgccca ggggattgat cgcggacggg aagttcatgt
     3601 gcaccacgtc gagcggcggc atgtcgtcgg cgcggggcat cgggtagtcc atcagggtgc
     3661 cggtcagcag ctggcccgcg tcgtcgtgga tcagctcctc gccgagcgcg ctgcccaggc
     3721 cctgggcgat gccgccgtgg agctggccct ccacgatcac cgggttgatg ggccgcccgc
     3781 agtcgtgcat cgcggccagc cggagcagcg tgatcgcgca cgcctcgagg tccacctcga
     3841 gcacgatgcc ctgcacgccg aaggcccacg tgacggtgtc cgggtagaag aaggcgcagg
     3901 cgctgagccc cggcccgccc gcctccgcga ccgcgcggct ccgcaccgcg gcgcgcgcga
     3961 cctggccgag cgacacgctc ttgcccggca cgccgcggac gcccacgcgc ccctcggcca
     4021 gcaccacgtc ctcgggcgcg cactcgaaca tctcggcgcc caccaggcgc gccttgtgcg
     4081 ccacctgcgc ggcggagcgc tgcaccgcgg gcccgcccac cgcggccacc cggctcgcga
     4141 tcgtgcccat gccgtagcct accaggctcg tgtcgccgcc caccaccgcc accgactcga
     4201 gcgcgacccc gagctcgtcg gccgcgatct gggccagcgt ggtctcgtgg ccctggccct
     4261 gggcgcagac cccgagatgc acgaacacgg tgccgtcggg gtccactcgg atgtcggcgc
     4321 cctcgaacgg gccgatgccg gtgccctcga cgtaggcgca gaggccgatg ccgatcggcc
     4381 gcgggccgcc gcgccgctcc gcctgccggg cgcgccagcc ggggtagtcg agggtcgcga
     4441 gcaggcgatc gaaggcggct gggtagtcgg ccgggtcgta gacgatgggc gcgccgtcgc
     4501 gataggtgag gccggtcggg accggcatct cgtcgggccg gatcaggttg aggcgccgga
     4561 gcgcggccgg atccatcccg atctcgcgcg ccgcgcggtc gagcagccgg tccaggacga
     4621 acgcggcctc gggccggccc gccccgcgat aggcggccgc gaaggtcttg tgggtgagga
     4681 cgttctgccc ggtcccgcgg tagtgcggca cgcggtacgg cccgggcagg tggttgatgg
     4741 tgttgagcgt gatggcctcg ccgagcgtcg gcgccacccc gtgatcgcgc gtgaacgcgg
     4801 tctcgatggc aatgatgcgc ccgtcgcgct cgagcccgat gcgcgcctcg tggacctggt
     4861 cgcggtcgcc cgccgcggtg aggcagtgct cgctgcgcgt ctcggcccac ttcaccggcc
     4921 ggccgagccg gcgggccacc accgcgacca ggatctcctc cggatagacg tggcccttga
     4981 ccccgaagcc gccgcccacg tcggggacga gcacgcggac gcgctcctcg gccaggcgga
     5041 ccaccggggc gatcgcgctc cgcaccgcga atggcacctg cgtcgacgtc cacaccgtga
     5101 gcccgccccc gatgggatcg ggcgcggcca ggacgccgcg ggtctcgagc ggcatgccgg
     5161 ccacgcgcgg gtaggcgaga cgcgcggcca ccacgaccgg ggcgcgggcg agcgcctcct
     5221 cgggctggcc cttggcgctg gcggtgaccg aggcgagatt gtccggccac gtctcgttga
     5281 cgcgcggcgc gccgggcgcg atggcggcgg cgggcgacgt cgcggcgggc aacgggtcgt
     5341 acgcgacgac cacgcgctcg acgccgtcgg cggccgtgta cggatcgctc gccaccacca
     5401 ccgccaccgg ctcgcccacg tggcgcacgc gcgcgccggc cagaaccggg tggacgtagg
     5461 gccgaccctt gggctccggc accagcggag gaacggtcgc gtcggccagc tccggcaggt
     5521 cgtccagggt gagcaccgcg acgactccgg ggacggcgcg cgcggccgcg gcgtcgatgg
     5581 cgcggacgcg cgcgtgggcg tgcgggctgc gcacgagggc caggtggagc aggccggcca
     5641 cccgcacgtc gtcgaggtag cggccgccgc cggcgacgaa gcggccgtct tccttccgct
     5701 tcggggaggc gccgaccagc accggggccg ggaccggaag cgcgctagcc gcgagcgccc
     5761 gccgcgcgca ggtgctcgtc gcgtcgggac tggagatcgc cgtcggtgta ggtcatcagc
     5821 tcgggccggg ccttgccgag catcacctgg aggtacgcgg gctcgaggcc cacgttggtg
     5881 aagccgtgga tcacgttggc cgggcaggag acgcagtccc acggcccgag caccgtctcg
     5941 gcgcggtggc cggccccgtc ctcgatgaag accagcacct tgcccgagag gatgaagaag
     6001 acctcctcga cctcgtgggt gtgcggggcg ttgccctggc cgggcgggat gaacatgacc
     6061 gagagcgtga agtgctgggc cgggatcacc gaggcgtccg tcttgccgga ggcgcccgag
     6121 ccgatgaagc ggtgctgggc gcgcttgaag ccctccacgc gcgcgtcggc gaacgcgtcc
     6181 cagtcggcgc ggcgctcggt gaaccgggcc acgtgggtgc gcagcgtctc gtccagggat
     6241 tgagcgggct gggcggtctg ggtcatgtgg tgtcctcccg gggatcacag tctagtcggt
     6301 cgcccgcggg cccgcaagac gcgcggccgc gtcttgcgtc catcgcgtct atccgttacg
     6361 atccgcgctg cgccgggcgt gcggctgtcg gcccggccgg gaggctcgtc caatgggcaa
     6421 gatcgtcttc gcgggcgcga tgtcgcacgt gctcgatccc gactactacc agcgcgcctg
     6481 cggcgacgag gggcgccgga tggtcaccga ctgcatgacc gagatcgccc ggatgggcga
     6541 gcgcttctcg gccaccggcg ccgacgcgct gatcgtggtg gccgacgacc acctgaacgc
     6601 gttctcgttc aacgcggtgc cggccctctg cgtccgcatc ggccgcaccg tgcagcgcat
     6661 gaagcaggat cacgcggagg cgttcgacgc ggtcctcgac cacatgccgg atgagtaccc
     6721 gctccacgag gatctcgcca accagattct ggaaaagggg ctcgggtccg gattcgacct
     6781 cgcgatgtcc tgggaggcgc ccgtcgatca cgcgttcctg agcccggtca acaccatgca
     6841 cgggacgcgg ccgatcccgc cgctggtgcc gatctgggtg aactgcttcg tggcgcccca
     6901 gccgaccccc aagcgctgct tcgacttcgg ccggcacatc gcccgcgtcg tcgcggcgtc
     6961 gccgtggaac gtgg
//
LOCUS       DATMCV010000678         7389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_103080, whole genome shotgun sequence.
ACCESSION   DATMCV010000678 DATMCV010000000
VERSION     DATMCV010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103080"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VNU03_15550"
     CDS             complement(<1..237)
                     /locus_tag="VNU03_15550"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75937.1"
                     /translation="MKLDVSMLTHDLKSIPDYARKVEAMGYDCLWSSETQHDPYLPLA
                     VAATSTSRIKLGTNIATVFSRSPMITAHTAWDLQK"
     gene            337..1560
                     /locus_tag="VNU03_15555"
     CDS             337..1560
                     /locus_tag="VNU03_15555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75938.1"
                     /translation="MPLYRGWSVVIACGTIAAFSWGLGFYGLGVYLHALSHLHGWSAG
                     LISLAVTVYYSLSAVCLVVVGGFIDRHGPRGVLTYGVLTMAAAVALLGLITAPWQLFA
                     VYVVMATAWSCLGSTGLSSTLLPWFGRRQGLALTLALTGASVGGMVLVPILVALVQRH
                     SFRFATTAVSVALLAIGLPLVWLVIGGRPTPAQVAVELGRDGAPAAVGERAQEEKVWT
                     RPAVLRVPSLWTLMVPFALALAAQVGFLVHQISVLEPYLGESRAALTVSATTVAALLG
                     RIVIGVLSDRVDLRKLSAVNIGAQGGALAVMAAWPSPTVLVVASLVFGLGVGNLITFP
                     PLLAREEFGQRSFGTVFGLVGAATQAGVALGPGLMGLLHDALGSYQTALWCLVALEGL
                     AAASVLGGRPRRADP"
     gene            1586..2251
                     /locus_tag="VNU03_15560"
     CDS             1586..2251
                     /locus_tag="VNU03_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75939.1"
                     /translation="MAKKGTGLLMVWADVPADKEEEFNRWYNEEHLPERMAIPGFLGG
                     ARYEAVKGGPKHLACYELESAAVMESPAYKKVQGNPTPWTKKIGPQAIGTPFIRNIYT
                     MLHPKALTPAIAGAPMAPALQIGRMDVPAAADREFNEWYNTVYVPNYEKVPGVIRGRR
                     YRAVEGTPTYLTLYEFEHPKASETAAWNAQRDASPVNATMRAHMTHAPGSPGVYVKTV
                     ELR"
     gene            complement(2229..3092)
                     /locus_tag="VNU03_15565"
     CDS             complement(2229..3092)
                     /locus_tag="VNU03_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75940.1"
                     /translation="MSAPRWRWLRLLLRRRIAMLGAVLVVLNVLVAVFAPAIGRWEPQ
                     RLDVQARLAPPTASHWMGTDDVGRDVWSRVIHGTRLSMIVGGAVVALSFVGGIVFGLL
                     GGYYRPLDNVLMRIMDGFMAFPGIILAIAMMASLGPSVVNVIVALGIVYVPRVARIVR
                     GSVLVIRETPYVEAARALGVPDLTVLRRHVLPNCLSPVIVQGTFIFAAAVLGEAALSF
                     LGVGVPPQIPSWGNVLAEGRLYLQQAPWLTLFPGAAIMGCILGLNLFGDGLRDLLDPK
                     MQGLATGVRPS"
     gene            complement(3103..4047)
                     /locus_tag="VNU03_15570"
     CDS             complement(3103..4047)
                     /locus_tag="VNU03_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75941.1"
                     /translation="MRTYLLRRVLAVIPVMLVVATVTFVLVHLAPGDPASIIAGPDAT
                     PENIERLRHHLGLDEPLPSQLVHWYGRLLRGDLGDSIFLRRPVTEAIVERLEPTLLLT
                     SWATLIAVLIGVPAGIVSARYHNTAVDQSFMGLALVGLSIPNFLLGLLMILVFGVWLR
                     WLPVAGYVPLDVGLWPNARSLLMPSVSLGLVQSALIARITRSSMLDVLREQYILASRA
                     KGLAERAVVYKHALKNAIIPTLTVIGITFAILVGGAVVIETVFNIPGLGRLIISAVLR
                     RDYPVIQGVVLVIAVTYTLINLAVDLVYLTLDPRIRYQ"
     gene            complement(4054..5613)
                     /locus_tag="VNU03_15575"
     CDS             complement(4054..5613)
                     /locus_tag="VNU03_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75942.1"
                     /translation="MNRMSRRAWFGSIVLAVLAGLVSTPAAWAQETPRMGGVLKVASI
                     GEPPTLDIPMSTATLVYEIMWHVNESLFTYDKSFTPVPLLADSWALTDKGLKYTINLR
                     KSVKFHNGKEMTSADVVPSLKRWGRVASVGRVLWKFVESVDAKDPYTVVISMKQPSSS
                     LIYGLSEPHAAIYPKESIEAAGEGQLKEFIGTGPFRFVEHRPDRHIKLARFKDYSARS
                     EPPNGFGGKRTAYFDEILFLTVPETAVRLAGVETGEYHHAMFIKQDSYERIKNIPTLE
                     SRIVKPRGWAVAVMNHKSPLMSQTKLRQAFQAALDMEPILVAGFGSRDFYRLDPGVFF
                     PEQPWHSTASAKLYNQHDKDKARRLLKEAGYAGQPIRWVTTREYEFMYKNALVAKQQL
                     EEVGMTVDMQVVDWATLNNRTEKADLWEVFSTGFVFSADPGSHVALRCTFNGWWCNAE
                     KERLLVELQGESDLKKRKAIIDRIQTVFYEDVGSVKLGDYFTLDVARRDLRGDFRTAP
                     RLYFWNSWLAK"
     gene            5738..7360
                     /locus_tag="VNU03_15580"
     CDS             5738..7360
                     /locus_tag="VNU03_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWP75943.1"
                     /translation="MYDYVIVGAGSAGCVLANRLSADPAVSVLLLEAGGPDTSPFIHT
                     PAMMGFLPDGPHDWRYRTVPQAHCNLRRFPWPRGRTLGGSSSINYMIYIRGHATDYDR
                     WAEAGNKGWSYEDVLPVFRKSEHNERMEDRYHGRGGPLNVADHRFRHPLSEMFVASAQ
                     ETGVPANPDFNGPVQDGAGFYQVTQKDGLRWSTASAFLRPALARPNLTVITGALTCRV
                     RFSGRRATGVEYVRHGQSALADAAREIVLCGGSVNSPQLLMLSGVGPADHLRAVGVDV
                     VADLPGVGKNLQDHLGTFVRNEITVPFSLYGATPEQLAQVQAEYEATRQGLFTSNIAE
                     AGAFLRTGGADGPPNLQGFFLPYCLTESPTEAFQPHCHGITFAFYVNRPASRGQITLA
                     SVDPLDQPLIDPAYLSDPADLPQFVAGIEMVRDIFRAKPLGDILGRELGPGAEARCEA
                     EIAAYVRDRASGTIFHPVGTCKMGTDPMAVVDPRLKVIGVEGLRVIDASVMPCVTTGN
                     TNAPTIMIGEKGAAMIKEDARAAISPRPAQAA"
BASE COUNT         1115 a         2595 c         2564 g         1115 t
ORIGIN      
        1 cttctgcaga tcccacgcgg tgtgggcggt gatcatcggg ctccgcgaga acaccgtcgc
       61 gatgttggtg ccgagcttga tccgggaagt ggacgtggcc gcgaccgcca gcggcaggta
      121 ggggtcgtgc tgcgtctccg acgaccacag gcagtcgtaa cccatggcct cgaccttccg
      181 ggcatagtcc gggatgctct tgagatcgtg ggtcagcatg gacacgtcga gcttcacggg
      241 cgtctccttt gaccgcgagt ctacctcacc ctctcccgtg aatcaccctc tcccccctgg
      301 ggagagggca gggtgagggg ctggtataac cgtcccgtgc ccttgtaccg cggctggtcc
      361 gtcgtcatcg cctgcggcac gatcgccgcc ttctcctggg gcctcggctt ctacggcctc
      421 ggcgtctacc tccacgcgct cagccacctg cacggctggt ccgccggcct catctcgctg
      481 gcggtgacgg tctactactc gctgagcgcc gtctgcctgg tggtggtcgg cggcttcatc
      541 gatcgccatg gtccccgtgg cgtgctcacc tacggggtgc tgaccatggc cgcggcggtc
      601 gcgctcctcg gcctgatcac ggctccgtgg cagctcttcg cggtctacgt ggtcatggcg
      661 accgcatggt cgtgtctcgg ctccaccggg ctcagcagca cgctcttgcc ctggttcggc
      721 cgccgccagg gtctggcact caccctggcg ctgaccggcg ccagcgtggg cggcatggtg
      781 ctggtgccga tcctggtcgc gctggtgcag cggcacagct tccggttcgc cacgacggcg
      841 gtgagcgtgg ccctgctcgc gatcggcctg ccgctcgtct ggctcgtgat cggcgggcgc
      901 ccgacgcccg cccaggtggc cgtcgagctc gggcgcgacg gtgcgccggc ggcggtgggt
      961 gagagagcgc aggaggagaa ggtgtggacg cgcccggcgg tgctgcgggt gccgagcctc
     1021 tggacgctga tggtgccctt cgcgctggcc ctggccgcgc aggtcggctt cctcgtccac
     1081 cagatcagcg tgctcgagcc ctacctcggc gagagtcgcg ccgcgctgac ggtgagcgcc
     1141 accacggtgg ccgccctgct cggccgcatc gtcatcggcg tgctctcgga ccgggtggac
     1201 ctgcgcaagc tctccgcggt caacatcggc gcccagggtg gggccctcgc ggtgatggcc
     1261 gcgtggccgt caccaaccgt gctcgtggtc gcgagcctcg tgttcggcct gggggtgggc
     1321 aacctgatca cgttcccgcc gctgctggcc cgcgaggagt tcggccagcg ctcgttcggc
     1381 accgtgttcg gcctggtcgg cgcggccacc caggcgggcg tggcgctggg ccccggcctg
     1441 atgggcctct tgcacgacgc gctcgggagc tatcagaccg cgctctggtg cctggtggcg
     1501 ctcgaagggc tggccgcggc ctccgtgctc gggggccggc cccgccgcgc cgatccgtga
     1561 tataccgtgc cgaaaggagg gcgacatggc caagaaaggc accgggctcc tgatggtgtg
     1621 ggccgacgtc ccggccgaca aggaagagga attcaaccgc tggtacaacg aggagcacct
     1681 gccggagcgc atggcgatcc ccgggttcct cggcggcgcg cgctacgagg cggtgaaggg
     1741 cgggccgaag cacctggcct gctacgagtt ggagagcgcc gcggtcatgg agagcccggc
     1801 ctacaagaag gtccagggaa atcccacccc gtggacgaag aagatcggcc ctcaggccat
     1861 cggcaccccg ttcatccgca acatctacac gatgctgcat ccgaaggcgc tgaccccggc
     1921 gatcgcgggt gcgccgatgg ccccggcgct ccagatcggg cgcatggacg tgcccgccgc
     1981 ggcggatcgc gagttcaacg agtggtacaa cacggtctac gtgccgaact acgagaaggt
     2041 gcccggggtg atccgcggcc ggcggtaccg cgcggtggag ggcacgccga cttatctgac
     2101 gctctacgag ttcgagcacc ccaaggcctc ggagaccgcg gcgtggaacg cccagcgcga
     2161 cgccagcccg gtgaacgcca cgatgcgcgc gcacatgacg cacgcgccgg gctcgcccgg
     2221 ggtctacgtc aagacggtcg aactccggta gcgagcccct gcatcttggg gtcgagcagg
     2281 tcgcgcaggc cgtcgccgaa gaggttcaac cccaggatgc agcccatgat cgcggcgccc
     2341 gggaacaggg tgagccaggg cgcctgctgc aggtagaggc gcccctccgc cagcacgttg
     2401 ccccaggacg ggatctgggg cggcacgccc acccccagga acgagagcgc ggcctcgccc
     2461 agcaccgcgg ccgcgaagat gaacgtgccc tgcacgatga ccggcgagag gcagttcggc
     2521 agcacgtggc ggcgcagcac ggtcaggtcg gggacgccca gggcccgcgc ggcctcgacg
     2581 tagggcgtct cgcggatcac cagcaccgag ccgcgcacga tgcgcgccac ccgtggcacg
     2641 tacacgatgc cgagcgccac gatgacgttc accacgctcg gcccgagcga ggccatcatg
     2701 gcgatggcca ggatgatgcc ggggaaggcc atgaacccgt ccatgatccg catcagcacg
     2761 ttgtcgagcg ggcggtagta gccgccgagc aggccgaaga cgatgccgcc cacgaaggac
     2821 agcgccacca ccgcgccgcc cacgatcatg gagaggcgcg tcccgtggat cacgcggctc
     2881 cacacgtcgc ggcccacgtc gtcggtgccc atccagtggc tcgcggtggg cggggcgagg
     2941 cgggcctgca cgtccaaccg ctgcggctcc cagcgcccga tcgcgggcgc gaacaccgcc
     3001 accagcacgt tgagcacgac caggaccgcg ccgagcatcg cgatccgccg ccgcagcagc
     3061 aggcggagcc accgccagcg cggcgcgctc actggtaccg gctcattggt agcggattcg
     3121 ggggtcgagg gtcaggtaga ccaggtccac cgccaggttg atcagggtgt aggtgaccgc
     3181 gatgaccagc accacgccct ggatcaccgg gtagtcgcgc cggagcaccg ccgagatgat
     3241 gaggcgcccg agccccggga tgttgaacac ggtctcaatc accaccgcgc cgcccaccag
     3301 gatcgcgaac gtgatgccga tcacggtcag ggtcgggatg atcgcgttct tcagcgcgtg
     3361 cttgtagacc accgcccgct cggccaggcc cttggcccgg ctggccagga tgtactgctc
     3421 gcgcaggacg tcgagcatgc tcgagcgcgt gatacgggcg atcagggccg actgtacgag
     3481 gcccagcgag acgctcggca tgagcagcga gcgcgcgttc ggccagagcc ccacgtcgag
     3541 cggcacgtag ccggccaccg gcagccagcg cagccagacc ccgaacacca ggatcatcag
     3601 gagcccgagc aggaagttgg ggatggagag gccgaccagc gccagcccca tgaacgactg
     3661 gtcgaccgcg gtgttgtggt agcgcgcgga gacgatgccg gccggcaccc cgatgagcac
     3721 cgcgatcagg gtggcccagc tcgtgagcaa gagcgtcggc tcgaggcgct ccacgatggc
     3781 ctcggtgacc gggcggcgca ggaagatcga gtcgcccagg tccccgcgca ggaggcgccc
     3841 gtaccagtgg acgagctggg agggcagggg ctcgtcgagg cccaggtggt ggcgtagccg
     3901 ctcgatgttc tccggcgtcg cgtccggccc cgcgatgatg ctggcggggt cgccgggagc
     3961 caggtggacc agcacgaacg tgacggtggc caccacgagc atcaccggga tgaccgccag
     4021 cacccggcgc agcaggtagg tccgcacgcc tccctacttg gccagccagc tattccagaa
     4081 gtacaaccgc ggcgcggtcc ggaagtctcc ccgcaggtcc cggcgcgcga cgtccagggt
     4141 gaagtagtcg ccgagcttga cgctccccac gtcctcgtag aacacggtct ggatgcggtc
     4201 gatgatcgcc ttccgcttct tgagatcgct ctcgccctgc agctcgacga gcaggcgctc
     4261 cttctccgcg ttgcaccacc agccgttgaa cgtgcagcgg agcgcgacgt ggctgcccgg
     4321 gtcggccgag aacacgaagc cggtggagaa gacctcccac aggtcggcct tctcggtgcg
     4381 gttgttcagg gtggcccagt cgaccacctg catgtcgacg gtcatgccca cctcttccag
     4441 ctgctgcttg gccaccagcg cgttcttgta catgaactcg tactcgcggg tggtcaccca
     4501 gcggatcggc tgcccggcgt agccggcctc cttgaggagg cggcgcgcct tgtccttgtc
     4561 gtgctggttg tagagcttgg cgctcgcggt ggagtgccac ggctgctccg ggaagaagac
     4621 gccggggtcc agccggtaga agtcgcgcga gccgaagccg gccaccagga tgggctccat
     4681 gtccagcgcg gcctggaagg cctggcggag cttcgtctgg ctcatcagcg gcgacttgtg
     4741 gttcatgacc gccaccgccc agccgcgcgg cttcacgatc cgcgactcca gcgtcgggat
     4801 gttcttgatg cgctcgtagg agtcctgctt gatgaacatc gcgtggtggt actcgccggt
     4861 ctcgaccccg gccagccgca ccgcggtctc gggcacggtg aggaagagga tctcgtcgaa
     4921 gtaggcggtg cgcttgcccc cgaagccgtt gggcggctcc gaccgggcgc tgtagtcctt
     4981 gaaccgggcc agcttgatgt gccggtccgg ccggtgctcc acgaagcgga aggggccggt
     5041 gccgatgaac tccttgagct gcccctcgcc ggcggcctcg atgctctcct tggggtagat
     5101 cgcggcgtgc ggctcggaca gtccgtagat gagcgagctc gacggctgct tcatgctgat
     5161 gaccaccgtg taggggtcct tggcgtccac gctctccacg aacttccaga gcacgcggcc
     5221 gacggaggcc acgcgtcccc agcgcttgag cgagggcacc acgtcggccg aggtcatctc
     5281 cttgccgttg tggaacttga cggatttccg aaggttgatc gtgtacttga ggcccttgtc
     5341 ggtgagcgcc cacgagtcgg cgaggagggg caccggcgtg aagctcttgt cataggtgaa
     5401 gagcgactcg ttgacgtgcc acatgatctc gtagaccagc gtggccgtgc tcatcgggat
     5461 gtcgagggtt ggtggctcgc cgatcgacgc gaccttgagc acgccgccca tgcgcggtgt
     5521 ctcctgggcc caggcagccg gggtcgagac caggcccgcg agcacagcga ggacgatgct
     5581 tccgaaccag gcccggcgcg acattcggtt catggacggt ctccttgggc tctccgagta
     5641 gcatcccgtc acggtcgctg tcaacaagcc ggccgcgatc gagcggcttg acaggtgcgg
     5701 gcggagcgcc tagggtcggc ctgccaggag gcccgccatg tacgactacg tgatcgtcgg
     5761 cgccggctcc gccggctgcg tcctcgccaa ccgcctctcc gccgatccgg ccgtcagcgt
     5821 gctgctgctc gaggccggcg gcccggacac ctcgcccttc atccacaccc ccgcgatgat
     5881 gggcttcctg ccggacggcc cgcacgactg gcgctaccgc acggtgcccc aggcgcactg
     5941 caacctgcgg cgcttcccgt ggccgcgggg gcggaccctc ggcggctcca gctcgatcaa
     6001 ctacatgatc tacatccgcg gccacgccac cgactacgat cggtgggcgg aagccggcaa
     6061 caaggggtgg agctacgagg acgtcctgcc cgtcttccgc aagtccgagc acaacgagcg
     6121 catggaagat cgctaccacg gccgcggcgg cccgctcaac gtggccgacc accgcttccg
     6181 tcacccgctc tccgagatgt tcgtggcctc ggcccaggag acgggcgtgc ccgccaaccc
     6241 cgacttcaac gggccggtcc aggacggcgc gggcttctac caggtcaccc agaaggacgg
     6301 gctgcgctgg agcaccgcga gcgccttcct gcggccggcg ctggcgcgcc cgaacctgac
     6361 cgtgatcacg ggagcgctca cctgccgggt ccgcttctcg ggccgacggg ccaccggcgt
     6421 cgagtacgtg cgccacgggc agtcggccct ggccgacgcc gcccgcgaga tcgtcctctg
     6481 cggcggcagc gtcaactcgc cgcagctgct catgctctcc ggcgtgggcc ccgccgacca
     6541 cctgcgcgcg gtcggcgtcg acgtggtcgc ggacctgccc ggggtcggca agaatcttca
     6601 agatcacctc ggtaccttcg tccgcaacga gatcacggtg ccgttctcgc tctacggggc
     6661 cacccccgag cagctcgccc aggtgcaggc cgagtacgag gcgacgcgcc agggcctctt
     6721 cacctcgaac atcgcggagg cgggcgcctt cctccggacc ggcggcgccg acgggccgcc
     6781 caacctgcag gggttcttcc tgccgtactg cctgaccgag tcgccgaccg aggcgttcca
     6841 gccccactgc cacggcatca cgttcgcgtt ctacgtgaac cgcccggcca gccggggcca
     6901 gatcacgctg gcctccgtcg atcccctcga tcagccgctc atcgatccgg cgtacctgag
     6961 cgatcccgcc gacctgccgc agttcgtggc ggggatcgag atggtgcgcg acatcttccg
     7021 ggcgaagccg ctcggcgaca tcctcgggcg cgagctgggc ccgggcgccg aggcgcgctg
     7081 cgaggccgag atcgcggcct acgtgcgcga ccgcgcctcc ggcaccatct tccacccggt
     7141 cggcacctgc aagatgggca ccgacccgat ggcggtggtc gatccgcgcc tcaaggtcat
     7201 cggcgtcgag gggctgcgag tcatcgacgc ctcggtgatg ccctgcgtca ccaccggcaa
     7261 caccaacgcg ccgacgatca tgatcggcga gaaaggcgcg gcgatgatca aggaggacgc
     7321 ccgcgcggcc atctcccctc ggccggcgca ggcggcctag tccagccgcg ccttacaccg
     7381 ccgccgtcg
//
LOCUS       DATMCV010000679         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_832704, whole genome shotgun sequence.
ACCESSION   DATMCV010000679 DATMCV010000000
VERSION     DATMCV010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832704"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VNU03_15585"
     CDS             <1..628
                     /locus_tag="VNU03_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HWP75944.1"
                     /translation="DWTNATFDPFASPAETGSAFGPKNGSNKRAVTRHRVTAKRMLGA
                     PGDEPVRTDESGFPINRHFTDVPQDAPEVHPEPGFEDQVVSVNLFAYLSRSDVTWNPS
                     VVSVCKHSLYCPTTEEYILPIIHGNDRVEWFVQLCDEIQWEVEDRDTGAVRARVTMKA
                     GDVAAMPADIRHRGFSPKRSMLLVWENASPELPALYAAGKMPPAPVQF"
     gene            652..1950
                     /locus_tag="VNU03_15590"
     CDS             652..1950
                     /locus_tag="VNU03_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75945.1"
                     /translation="MSDETRRGGRREFLRVAAGSAAAITLGARPGRAAAQRGTRTLKI
                     GYVSPQTGPLAGFGETDTFVVGGIRKALGKGLAVRGQSFPVEVLVRDSQSDPNRAAEV
                     ASRLILSDKVDLMLVSSTPETTNPVSDQCEANGTPCVSSVCPWQPWFFGRGGKPETGF
                     KSTYHFFWGLEDIIGVFVDMWNTLPTNKVVGALWPNDGDGNAWGDPQRGFPPALASAG
                     YKLVDPGRYPNLSDDFSAQISTFKKEKVEIVTGVPIPPDWTTFWKQAAQQGFKPKIAT
                     VGKALLFPRSVEALGDLGDGMSTEVWWTPRHPFKSSLTGASAADLAAAYTKETKKQWT
                     QPLGFTHALFEVAADVLKRAASADDKGAVIAAIKATDLGTIVGPISWAKGPVPNVTKT
                     PLVGGQWGRGKDFRFDVAVVSNRAAPSIPASAKLRPIGQA"
     gene            1961..2686
                     /locus_tag="VNU03_15595"
     CDS             1961..2686
                     /locus_tag="VNU03_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018000574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP75946.1"
                     /translation="MSALLALRSVSKAFGSLRVIDDLSLSVAPGEALGVIGPNGAGKT
                     TALNLLIGRLRPERGAVVFDERDVTRVATHARCRAGIALTHQIPHPFEALTVFENVLV
                     GGIFGGGRAERAASAPALAALELTGLAGRANTRAGTLTLLERKRLELARALATAPRVL
                     LLDEIAGGLGEQEVQELVEVIRRVHREGVAIVWIEHIVHALRSVVSRLAVISAGRLLT
                     EGAPDDVMRSPEVQRVYLGIDVQ"
BASE COUNT          402 a         1006 c          962 g          383 t
ORIGIN      
        1 cgactggacg aacgcgacct tcgacccctt cgcgtccccc gcggagaccg gcagcgcctt
       61 cgggcccaag aacggctcca acaagcgcgc ggtcacccgc caccgggtca ccgccaagcg
      121 gatgctcggg gcgcccggcg acgagccggt gcgcaccgac gagagcggct tcccgatcaa
      181 ccgccacttc accgacgtgc cgcaggacgc gcccgaggtc caccccgagc ccggcttcga
      241 ggaccaggtg gtctcggtga acctcttcgc ctacctctcg cgctcggacg tgacctggaa
      301 cccgtcggtg gtgtcggtgt gcaagcacag cctctactgc cccaccaccg aggagtacat
      361 cctgccgatc atccacggca acgaccgggt ggagtggttc gtgcagctct gcgacgagat
      421 ccagtgggag gtggaggacc gcgacaccgg cgcggtgcgg gcccgcgtca ccatgaaggc
      481 gggcgacgtg gcggccatgc cggccgacat ccgccaccgc ggcttctctc ccaagcgctc
      541 gatgctcctg gtgtgggaga acgcctcgcc ggagcttccc gcgctctacg cggccggcaa
      601 gatgccgccg gcccccgtgc agttctagcc cgacgcggca ggaggacgac gatgtccgac
      661 gagacgaggc ggggcggccg gcgggagttc cttcgagtgg ccgcgggctc cgcggccgcg
      721 atcaccctcg gggcgcggcc gggccgggcc gcggcccagc gcgggacgcg gacgctcaag
      781 atcggctacg tgagcccgca gaccgggccc ctggccggat tcggcgagac cgacaccttc
      841 gtagtcggcg gcattcgcaa ggcgctcggg aagggcctcg cggtgcgcgg ccagagcttc
      901 ccggtcgagg tgctggtgcg cgacagccag tccgatccga accgcgccgc cgaggtcgcc
      961 tcgcgcctca tcctgtcgga caaggtcgac ctcatgctgg tgtcgagcac gccggagacg
     1021 acgaacccgg tctccgacca gtgcgaggcc aacggcacgc cgtgcgtctc gagcgtctgc
     1081 ccgtggcagc cgtggttctt cggccgcggc ggcaagccgg agaccggctt caagtccacc
     1141 tatcacttct tctggggcct cgaggacatc atcggggtct tcgtcgacat gtggaacacg
     1201 ctgcccacca acaaggtggt gggcgcgctc tggccgaacg acggcgacgg caacgcgtgg
     1261 ggcgaccccc agcgcggctt cccgcccgcg ctggcctccg ccggctacaa gctcgtcgat
     1321 cccggacgct atccgaatct ctccgacgac ttctcggccc agatctccac cttcaagaag
     1381 gagaaggtcg agatcgtcac cggcgtgccg atcccgccgg actggacgac gttctggaag
     1441 caggcggccc agcagggctt caagcccaag atcgccaccg tcggcaaggc gctcctgttc
     1501 ccgcgctcgg tcgaggccct cggcgacctg ggggacggta tgtccaccga ggtgtggtgg
     1561 acgccgcggc atcccttcaa atcctcgctg accggcgcga gcgcggccga cctggctgcc
     1621 gcctacacga aggagacgaa gaagcagtgg acccagcccc tcggcttcac ccacgccctc
     1681 ttcgaggtgg ccgcggacgt cctcaagcgc gccgcctccg cggacgacaa gggcgcggtg
     1741 atcgcggcga tcaaggcgac cgatctcggc acgatcgtgg gcccgatcag ctgggccaag
     1801 gggccggtgc ccaacgtcac caagaccccg ctcgtgggcg ggcagtgggg gcgcggcaag
     1861 gacttccgct tcgacgtggc ggttgtcagc aaccgggccg ccccgagcat ccccgcctcc
     1921 gccaagctcc ggcccatcgg ccaggcgtga gcgtccgcgg ctgagcgcgc tgctcgcgct
     1981 gcggagcgtc tcgaaggcgt tcggctcgct gcgggtcatc gacgatctga gcctctcggt
     2041 cgcgccgggg gaggcgctcg gggtcatcgg gccgaacggc gcgggcaaga ccaccgcgct
     2101 caacctcttg atcggccgtc tgcggcccga gcgcggtgcc gtcgtcttcg acgagcgcga
     2161 cgtcacccgg gtcgccacgc acgcacgctg ccgggccggc atcgcgctga cccaccagat
     2221 cccgcacccc ttcgaggcgc tcaccgtgtt cgagaacgtc ctcgtgggcg ggatcttcgg
     2281 gggcggacgg gccgagcgcg cggccagcgc tcccgccctg gctgccctcg agctgaccgg
     2341 cctcgcgggg cgggccaaca cccgcgcggg caccctgacc ctgctggagc gcaagcgtct
     2401 cgagctggcc cgcgcgctcg ccaccgcgcc ccgggtgctg ctgctcgacg agatcgcggg
     2461 cggcctcggc gagcaggagg tgcaggagct cgtggaggtg atccggcgcg ttcaccggga
     2521 gggggtcgcg atcgtgtgga tcgagcacat cgtgcacgcg ctgcgctcgg tggtgagccg
     2581 gctcgcggtc atcagcgcgg ggcgcctgct gaccgagggc gcgcctgacg acgtgatgcg
     2641 gagcccggag gtccagcgcg tctacctggg catcgacgtg cagtgagcct cctccaggtc
     2701 cagcgcctga gcgccttcta cggcagcttc caggccctct tcgacgtctc gct
//
LOCUS       DATMCV010000680         4340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_832734, whole genome shotgun sequence.
ACCESSION   DATMCV010000680 DATMCV010000000
VERSION     DATMCV010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832734"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /gene="treS"
                     /locus_tag="VNU03_15600"
     CDS             <1..1673
                     /gene="treS"
                     /locus_tag="VNU03_15600"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009269 - response to desiccation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HWP75947.1"
                     /translation="TEAQYQSMVRQAGDNGAVIIGDVIPGHSGKGADFRLAERRYGEY
                     PGLYHMVEIRPADWGLLPAVPAGKDSINLGPAAVDALKAAGYIVGQLPRTIFYEPGVK
                     ETDWSATDAVVGADGTSRRWVYLHYFKEGQPTFNWLDPTFAAPRLVAGDAIHSLGTLG
                     ERMLRLDANGFLGIEVGPNNGPAWSEGHPLSVTANQLIAGLVRKLGGFTFQELNLTLE
                     DMKAMSQGGADLSYDFITRPAYQHALVTGDASFLRVMLDLQRAYQVDPAALVHALQNH
                     DELTLELVHFWTRYKDTTFTFRGKPVKGGALRETIRAEMHARLQGLAAPYNLKAANGI
                     SSTTATVAAAALGIRDVARLTEAQRHDIQRAHLLLALYNAMQPGVFALSGWDLVGALT
                     LPAESVQSLIADGDTRWINRGAYDLLGKHPGVTKSSSDLPRAQALYGPLPEQLARPDS
                     FASELRKMLRVRAQYRINESEQVELPAVQARGLIVMVHRLPAGAGIQVTAVNFGRAAV
                     RETVTIRTAPADGAVTDLLAEKGLGKLGPARRLPVALGPHQGQALLIK"
     gene            1674..3032
                     /locus_tag="VNU03_15605"
     CDS             1674..3032
                     /locus_tag="VNU03_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP75948.1"
                     /translation="MRPPLSFWQIWNMCFGFLGIQFGWALQMANMSAIYEYLGADAHQ
                     IPILWLAAPLTGLLVQPVIGHLSDRTWNRLGRRRPYFLTGALLASFALIAMPHSGTLW
                     MAAGLLWVMDASINISMEPFRAFVADLLPPEQRTRGFAMQALFIGLGAVIASMLPWLL
                     YGLFPPGSDGGTSGKAIPFAVRLAFYAGAVAFLAAVLWTIFTTREHPPEDLEAFRQMK
                     AEKAGFRAAVVEIFHDVLAMPPTMRRLAWVQIFTWLGFFCMWLYFGVAVARNIFGGAP
                     GSPAYDQGIAWAGNCFAMYSAVCFAFSFALPAAAARLGRRLTHGLCLLMGALGLVSVA
                     FVDDKYLLLLPMVGVGVAWASTLAMPYAILAGALPAGKTGIYMGIFNFFIVIPEILAA
                     LGFGTLLERLLTGDSALVQLVGGDNRLTVVVIGGLSLAVAAALCTTVIEPEAERAEAE
                     RE"
     gene            complement(3387..>4340)
                     /locus_tag="VNU03_15610"
     CDS             complement(3387..>4340)
                     /locus_tag="VNU03_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWP75949.1"
                     /translation="VVILPALAVAEQIGLPLPAMPALLAVGALAAHGRISIPLVLGAI
                     SAAALATDFAWYELGRRRGARVLARLCQLSLAPDSCLHRAQSIFARYGARSMLVAKFV
                     PGLTTVMPPLAGVFAVARGWFALYDLAGVLLWAGTWLGIGYFFSDAIVLIAARATALG
                     HMLGLVIATALAGYILVKYGRRHLFLGRLRTERVSPEALKRQLDAGEDIAIIDLRSPL
                     EMTATPYAIPGNRWLAADVIDEHEAQLLGSRDVVLYGASSNDPTSARVALQLKGKGIT
                     RARPLEGGLAAWMALKFPVRPVQLPAVAADGSSASVRSTAA"
BASE COUNT          667 a         1573 c         1447 g          653 t
ORIGIN      
        1 gcacggaggc acagtaccag agcatggtcc ggcaggcggg cgacaacggc gccgtgatca
       61 tcggcgatgt catccccggc cacagcggca agggcgccga cttccgcctc gccgagcggc
      121 gctacggcga gtatccgggc ctctaccaca tggtggagat ccgccccgcg gactggggcc
      181 tgctcccggc cgtgcccgcc ggcaaggact cgatcaacct cgggcctgcg gcggtcgacg
      241 ccctcaaggc ggccggctac atcgtcgggc agctcccgcg cacgatcttc tacgagccgg
      301 gcgtcaagga gaccgactgg tcggccaccg acgccgtcgt cggcgccgac ggcacgagcc
      361 ggcgctgggt gtacctgcac tacttcaagg aagggcagcc caccttcaac tggctcgacc
      421 ccaccttcgc ggcgccgcgg ctggtggcgg gtgacgccat ccactcgctc ggcaccctgg
      481 gcgagcggat gctgcgtctc gacgccaacg gcttcctcgg catcgaggtc ggcccgaaca
      541 acgggcccgc ctggtccgag ggccacccgc tctcggtcac cgccaaccag ctcatcgccg
      601 gcctggtgcg caagctcggc ggcttcacct tccaggagct caacctgacg ctcgaggaca
      661 tgaaggcgat gtcccagggc ggcgccgatc tctcctacga cttcataacc cgcccggcct
      721 accagcacgc cctcgtcacc ggcgacgcct cgttcctgcg ggtcatgctc gacctgcagc
      781 gggcctacca ggtggacccg gcggccctcg tccacgcgct ccagaatcac gacgagctca
      841 ccctcgagct ggtgcacttc tggacccggt acaaggacac cacgttcacc ttccgcggca
      901 agccggtgaa gggcggcgcc ctgcgcgaga cgatccgcgc ggagatgcac gcccgcctcc
      961 agggcctcgc cgcgccctac aacctgaagg cggccaacgg catctccagc accaccgcca
     1021 ccgtggccgc cgccgccctc ggcatccgcg acgtcgcccg gctcaccgag gcgcaacggc
     1081 acgacatcca gcgcgcccat ctgctcctgg cgctgtacaa cgccatgcag cccggcgtct
     1141 tcgcgctctc cggctgggat ctggtgggcg ccctgaccct gcccgccgag tccgtgcaat
     1201 ccttgatcgc cgatggcgac acgcgctgga tcaaccgggg cgcctacgac ctgctgggca
     1261 agcacccggg cgtgacgaag tcgagcagcg acctgccgcg cgcccaggcc ctctacggcc
     1321 cgctgccgga gcagctcgca cgcccggact cgttcgcctc cgagctccgc aagatgctgc
     1381 gcgtgcgcgc gcagtaccgg atcaacgaga gcgagcaggt cgagctgccg gcggtgcagg
     1441 cgcgaggcct gatcgtcatg gtgcaccgcc tgcccgccgg cgcgggcatc caggtgaccg
     1501 cggtcaactt cggccgcgcg gccgtccggg agaccgtgac catccggacc gcgccggcgg
     1561 acggggcggt caccgacctc ctcgcggaga agggcctggg caagctcggc cccgcgcgac
     1621 gcctgcccgt tgccctcggt ccgcaccagg ggcaggccct gctcatcaag tagatgagac
     1681 ccccgctgag cttctggcag atctggaaca tgtgcttcgg cttcctgggc atccagttcg
     1741 gctgggcgct ccagatggcc aacatgagcg cgatctacga gtacctcggc gccgacgcgc
     1801 atcagatccc catcctgtgg ctcgccgccc cgctgaccgg gctgctcgtg cagcccgtca
     1861 tcggccacct gagcgaccgc acctggaacc gcctgggccg gcgccgcccc tacttcctca
     1921 ccggcgcgtt gctcgcctcc ttcgcgctga tcgccatgcc gcactcgggc acgctctgga
     1981 tggcagccgg cctgctgtgg gtgatggacg cctccatcaa catcagcatg gagcccttcc
     2041 gcgccttcgt cgccgacctg ctcccgccgg agcagcgcac gcgcggcttc gccatgcagg
     2101 cgctcttcat cgggctgggc gcggtcatcg cctcgatgct cccctggctg ctctacggtc
     2161 tcttcccgcc cggcagtgat ggcggcacct ccggaaaggc cattccgttc gcggtgaggc
     2221 tggccttcta cgcgggcgcg gtcgccttcc tggccgccgt gctctggacc atcttcacga
     2281 cccgcgagca tccgccggag gacctggagg ccttccggca gatgaaggcc gagaaggccg
     2341 gcttccgcgc cgcggtcgtc gagatcttcc atgacgtgct ggcaatgccg ccgaccatgc
     2401 ggcgactggc ctgggtgcag atcttcacct ggctgggctt cttctgcatg tggctctact
     2461 tcggagtcgc ggtggcccgg aacatcttcg gcggcgcgcc cggctcgccg gcctacgacc
     2521 agggcatcgc ctgggccggc aactgcttcg ccatgtactc ggccgtctgc ttcgccttct
     2581 cgttcgcgct gccggcggcg gccgcacggc tgggccgccg cctcacccac ggcctgtgcc
     2641 tgctgatggg cgccctcggc ctggtctcgg tggccttcgt cgacgacaag tacctgctgc
     2701 tcctgcccat ggtgggcgtg ggcgtggcct gggccagcac cctcgcgatg ccgtacgcca
     2761 tcctggccgg cgccctgccg gccgggaaga ccggcatcta catgggcatc ttcaacttct
     2821 tcatcgtcat cccggagatc ctggccgcgc tcggcttcgg cacgctgctg gagcgtctcc
     2881 tcaccggcga cagcgcgctg gtgcagctgg tcggtggtga caaccggttg acggtcgtcg
     2941 tcatcggcgg cctctcgctc gcggtggcgg cggcgctctg cacgaccgtc atcgagcccg
     3001 aagccgagag ggccgaagcc gagagggaat gacgagcaag gcagccaccc acgcaatgac
     3061 gagattaggt tagattgtgg gcccacaact ctcacgccag caccggggaa agggtcgcga
     3121 ccaatgggta tgccaaacgg aggaatcgtc acgggactct tggtggtgaa cattctggcc
     3181 ggatgcagca cgagcgaacc gatcgtctat gtgaagccgg gggtcaccgc gagtcagcag
     3241 aaggccgatc ggaccgcatg cgccgaagcc tcgatcggtg tcatctcgga cccgcggccg
     3301 tcgccactgc ccccgatcga tcgggaggct ttcgcacgat gcatgcagac gaagggatac
     3361 actccagcgc agaagtagaa ggccggctac gcagcggtgg atcgtacgct ggccgagctc
     3421 ccgtcagcgg cgacggccgg gagctgaacc ggtcgaacgg gaaactttag ggccatccac
     3481 gcggccagtc cgccctcgag cggccgggcc cgagtgatcc ccttaccttt cagctgaagt
     3541 gccacccggg cgctcgtcgg gtcattggat gaggcgccgt agagcaccac atcccgagat
     3601 ccgaggagct gtgcctcgtg ctcatcgatg acgtcggcgg ccagccagcg gtttcccggg
     3661 atggcgtaag gtgtggccgt catctcgagc ggactccgta gatcgatgat cgcgatgtct
     3721 tctccggcgt cgagctgccg cttgagcgct tcgggggaga cgcgctccgt ccggagcctg
     3781 cccagaaaca ggtggcggcg cccgtacttg accaggatgt acccggcgag cgcagtcgcg
     3841 atgaccaggc cgagcatatg ccccagcgcg gtggctcggg cggcgatcag gacgatggcg
     3901 tcgctgaaga agtatccgat gcccagccac gtgcccgccc agagcagcac gcccgcgagg
     3961 tcgtagagag cgaaccaccc ccgtgccacc gcgaacacgc cggcgagcgg cggcatgacc
     4021 gtggtcaagc cgggcacgaa cttcgcgacg agcatgctcc gtgcgccgta tcgggcgaag
     4081 atgctctggg cccgatggag gcacgagtcg ggcgcgaggg acagctggca gagtctcgcc
     4141 agcacccttg ccccgcgacg acggccgagc tcataccagg cgaagtcggt ggccagggcc
     4201 gcggccgaga tcgccccgag cacgagcggg atgctgatgc ggccgtgcgc ggccagggcc
     4261 ccgaccgcga ggagggccgg catggcgggg agcggcaggc cgatctgctc ggcgacggcc
     4321 agggcgggca ggatcacgac
//
LOCUS       DATMCV010000681         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_769316, whole genome shotgun sequence.
ACCESSION   DATMCV010000681 DATMCV010000000
VERSION     DATMCV010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769316"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VNU03_15615"
     CDS             complement(<1..815)
                     /locus_tag="VNU03_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75950.1"
                     /translation="MWRVIRGALPARHRGVTVAGLLVVGVLALAAGLASIVVPGSPTE
                     IARDLALRSPTWAQPFGMDDLGRSMLSRVVHAYRVSLGVALGSVGLALLAGVPLGLVA
                     GYAEGALDQLIMRPLDVLMSFPAILLAIALMSVFGTGVVLVAVSLAVVYTPIVARVVR
                     AAVLAVKVEVYVDGARALGAPVPRLVLRHILPNAATPILVQASVLMAIAILVEASLSF
                     LGVGVRPPTPSLGLMLADGRGFMVQAPWMVIFPGFAIMAAVLGFNMIADGLRD"
     gene            complement(832..1776)
                     /locus_tag="VNU03_15620"
     CDS             complement(832..1776)
                     /locus_tag="VNU03_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75951.1"
                     /translation="MILYGVRRLALAVPLLLGMSVLIFGLMRLVPGDPAVAVLGYKAT
                     PEGIAALREAFHLDEPVPRQYLRWLGAAARGDLGHDFRQNEPIGRMILDRLPVTLELA
                     GLAALCTALIGVPLGLLAGGRRGGGTDRAALAVGLIGVSIPDFWLGIMLILGLSLGAG
                     LLPSGGWVPFAESPIDNLIHLTLPALTLAFSRAAVLGRLTRAAVLDTMHRGFVQYARA
                     KGLGERAILFRHVLPNAAIPIVTVLGLQVGYMLGGAIVVEMIFTLPGLGRMTLDAVLE
                     RNYPVVQSAVLVIGAMFMLVNLATDVLYGAIDPRMRQR"
     gene            complement(1843..>2025)
                     /locus_tag="VNU03_15625"
     CDS             complement(1843..>2025)
                     /locus_tag="VNU03_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75952.1"
                     /translation="FTASPAARKKMYPELVRWFQEEGSLVVFSNELQRYWMKPNVQGS
                     TPLSSLELRFEDTWLG"
BASE COUNT          350 a          745 c          719 g          211 t
ORIGIN      
        1 cagtcgcgga gaccgtcggc gatcatgttg aaaccgagga ccgcggccat gatcgcgaag
       61 ccggggaaga tcaccatcca gggggcctgc accatgaacc cgcgcccgtc ggccagcatg
      121 agaccgagcg acggggtggg cgggcgcacc ccgaccccca ggaacgacag cgacgcctcg
      181 accaggatcg cgatggccat cagcaccgag gcctgcacca ggatcggcgt cgccgcgttc
      241 ggcaggatgt gccgcagcac caggcggggc accggcgccc ccagcgcgcg ggcaccgtcg
      301 acgtacacct ccaccttgac cgcgaggacc gcggcccgca ccacccgggc cacgatcggc
      361 gtgtagacca ccgcgagcga caccgccacc agcaccacgc cggtgccgaa gacgctcatc
      421 agcgcgatcg cgagcaggat ggccgggaac gacatcagca cgtcgagcgg tcgcatgatg
      481 agctggtcga gcgcgccctc cgcgtagccc gcgaccaggc cgagggggac gccggccagc
      541 agcgccaggc ccaccgagcc gagggccacc ccgagcgaga cccggtaggc gtgcacgacc
      601 cggctcagca tgctgcgccc gaggtcgtcc atcccgaagg gctgcgccca cgtcggagag
      661 cgcagcgcga gatcgcgggc gatctcggtc gggctgccgg gcacgacgat cgacgcgagc
      721 ccggcggcga gcgcgaggac gcccaccacg agcagcccgg ccaccgtgac cccgcggtgc
      781 cgcgcgggca acgccccgcg aatcacccgc cacatcagcg gcgccgcacc atcagcgctg
      841 ccgcatgcgc ggatcgatag ccccgtagag cacgtcggtg gccaggttca ccagcatgaa
      901 catcgcgccg atcaccagca ccgcgctctg caccaccggg tagttgcgct cgagcaccgc
      961 gtcgagggtc atgcggccga gcccgggcag cgtgaagatc atctcgacca cgatggcccc
     1021 gcccagcatg tagcccacct gcaggccgag caccgtgacg atcgggatgg ccgcgttggg
     1081 cagcacgtgg cggaacagga tggcgcgctc gcccagcccc ttggcgcgcg cgtactgcac
     1141 gaacccgcga tgcatggtgt cgagcaccgc ggcccgggtg agccggccga gcaccgcggc
     1201 ccggctgaac gcgagggtca gcgcggggag cgtcaggtgg atcaggttgt cgatcgggct
     1261 ctccgcgaag ggcacccagc cccccgaggg caggagcccc gcccccagcg agagcccgag
     1321 gatcagcatg atgccgagcc agaagtcggg gatggagacc ccgatcaggc ccaccgcgag
     1381 cgcggcgcgg tccgtcccac cgccgcgccg gccgccggcc aggaggccga gcgggacgcc
     1441 gatcagcgcg gtgcagagcg cggccaggcc ggccagctcg agggtgaccg gcagccggtc
     1501 gaggatcatc cgcccgatcg gctcgttctg gcggaagtcg tggccgaggt cgccgcgcgc
     1561 ggcagcgccg agccagcgca ggtactgccg gggcaccggc tcatcgaggt ggaatgcctc
     1621 ccgcagggcc gcgatgccct cgggagtggc cttgtagccg agcaccgcca ccgcggggtc
     1681 gccgggcacc aggcgcatga gcccgaagat cagcacgctc atgccgagca gcagcggcac
     1741 cgccagggcc agccgccgca ctccgtacag aatcatctcg cgcgtgttcg aggcggtggg
     1801 gggtctgggg gaggagcggc gctgcgctcc cccccagtca gatcaaccca gccaggtgtc
     1861 ctcgaagcgc agctcgagcg acgacagagg cgtcgagccc tggacgttgg gcttcatcca
     1921 gtagcgctgc agctcgttgc tgaagacgac cagcgagccc tcctcctgga accagcgcac
     1981 cagctccggg tacatcttct tgcgcgcggc cgggctcgcg gtgaa
//
LOCUS       DATMCV010000682         2329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_699770, whole genome shotgun sequence.
ACCESSION   DATMCV010000682 DATMCV010000000
VERSION     DATMCV010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699770"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1739)
                     /locus_tag="VNU03_15630"
     CDS             complement(150..1739)
                     /locus_tag="VNU03_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75953.1"
                     /translation="MKKHPWARLVMLGLVLAALAPATGRAQAPERFGTVDFPNSCAPA
                     VQEAFLRSVAMLHSFNYGPGERAFRDVLAQDPSCAIATWGIAAILMNNPLAGIGAPKE
                     WAERAQAAIDEGRKIGAKTARERDYIEAVAAYYEDWANRPERTRQQNRAKAFEALAAR
                     YPGDDEAQIFYALYLASTQSLTDQTYKAYLTAADILEKQFVKHPDHPGVAHYLIHSYD
                     APPIAAKGVPAARRYASIAPAAPHALHMPSHIFTRVGLWTESAETNARSAASARRDGD
                     GDEQAHAMDYMVYAYLQLARDTDARRVLDERGQVARFVRFAGPYALAAIPARYAVERG
                     DWAAATKLEPTPSAFPFTEALTHFALGLGAARTGDAAGAEKEVQELARLRDALRAAKS
                     EYWATEVEVNRLGVAAWAALARGKSDDALALMRSAADAEDRSEKHIVTPGRILPAREM
                     LGDMLLELKRPAEALKEFEAAQLREPGRFRGYAGAARAALQSGDRTKARHYYVKLVDM
                     AGAGDARPDLTQARAFLAANP"
     gene            complement(1830..>2329)
                     /locus_tag="VNU03_15635"
     CDS             complement(1830..>2329)
                     /locus_tag="VNU03_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWP75954.1"
                     /translation="TKSRPPSKTISAGTRESERGLGDLFVIRVAGNIVAASQIGSVEF
                     AAERFGTRLVVVLGHSQCGAVRATLEELQQPTGSQSRNLRSIVDRVRPSVEALLSTEL
                     RHDPEALVRHAVRANVRVSASHLRHGSEVLEQLIQRDGLLVVGAEYSLETGAVEFFDG
                     VPKSV"
BASE COUNT          299 a          831 c          845 g          354 t
ORIGIN      
        1 agcgcggacg gacatccaga agctgggcgg ccgcctcgcc ttcgccaacg cctacctgtc
       61 ggtgggtgac gagcggatcg tgcgggccag cgcggtcttc gtgcgcggcg gccgccccgg
      121 ggcctagcgg ctctgcctcc cgccgcgcct caggggttgg cggccaggaa cgcgcgcgcc
      181 tgggtgaggt cgggccgcgc gtcgcccgcg cccgccatgt cgaccagctt gacgtagtag
      241 tgccgcgcct tcgtccggtc gccgctctgt agcgcggcac gggcggcgcc cgcgtagccc
      301 cggaagcgcc cgggctcgcg cagctgggcg gcctcgaact ccttcagggc ctccgccggc
      361 cgcttcagct cgagcagcat gtcgccgagc atctcgcggg cgggcaggat gcgcccgggg
      421 gtcacgatgt gcttctcgct gcggtcctcc gcgtccgccg cgctccgcat cagggcgagc
      481 gcgtcgtccg acttgccccg cgcgagcgcg gcccacgcgg ccacccccag gcggttcacc
      541 tcgacctcgg tggcccagta ctcgctcttg gccgccctga gcgcgtcgcg cagccgggcc
      601 agctcctgca cttccttctc ggcgcccgcc gcgtcgccgg tgcgggccgc cccgagcccg
      661 agcgcgaagt gggtcagcgc ctccgtgaac gggaacgcgc tgggggtcgg ctcgagcttg
      721 gtggccgccg cccagtcgcc ccgctcgacc gcatagcggg ccgggatggc ggcgagcgcg
      781 tagggcccgg cgaagcggac gaaccgcgcc acctggcctc gctcgtcgag cacccgccgc
      841 gcgtccgtgt cgcgcgcgag ctgcagatag gcgtagacca tgtagtccat cgcgtgggcc
      901 tgctcgtcac cgtcgccgtc gcgccgggcc gacgcggcgg agcgggcgtt ggtctcggcc
      961 gactcggtcc agagcccgac gcgggtgaag atgtgcgacg gcatgtgcag ggcgtgcggc
     1021 gcggcgggcg cgatggaggc gtagcggcgc gcggccggga cgcccttcgc cgcgatcggg
     1081 ggcgcgtcgt agctgtggat caggtagtgg gcgaccccgg gatggtccgg atgcttcacg
     1141 aactgcttct cgaggatgtc ggccgcggtc aggtaggcct tgtaggtctg gtcggtgagg
     1201 gactgggtgg acgcgaggta gagcgcgtag aagatctgcg cctcgtcgtc gcccgggtag
     1261 cgggcggcga gcgcctcgaa ggccttcgcg cggttctgct ggcgggtgcg ctccgggcgg
     1321 ttggcccaat cttcatagta ggccgcgacc gcctcgatgt agtcgcgctc gcgcgcggtc
     1381 ttcgccccga tcttccgccc ctcgtcgatg gcggcctggg cgcgctcggc ccactccttg
     1441 ggcgctccga tgccggcgag cgggttgttc atcaggatgg ccgcgatgcc ccaggtggcg
     1501 atggcgcatg acggatcctg ggcgagcacg tcccggaagg cgcgctcgcc ggggccgtag
     1561 ttgaaggagt gcagcatggc gaccgagcgc aggaacgcct cctgtaccgc cggcgcgcag
     1621 gagttcggga agtcgaccgt cccgaaccgc tccggggcct gcgcccggcc ggtggccggc
     1681 gcgagcgctg caagtacgag gcccagcatc acgagccgcg cccacgggtg cttcttcatc
     1741 gtcgtctcct tcgcgcgagg tgcccggtcg atcgtcggct ccatcctatc ccagctcgag
     1801 gcgcggcgga cgtgtcctgc gcgcggggct caaacgctct tcggcacgcc gtcgaagaac
     1861 tccaccgccc cggtctccag ggaatactcg gcgcccacga cgagaagccc atctctctgg
     1921 atcaattgct ccagaacttc cgacccgtgt cgcagatggc ttgccgagac gcggacgttg
     1981 gcccgaaccg cgtgccgcac cagagcttcc gggtcgtgtc ggagctctgt cgacagcaac
     2041 gcctccacgg atggtcgaac ccggtcgacg atcgagcgca ggttccgcga ctggctgccc
     2101 gtcggctgct gaagctcctc cagcgttgcc cgaaccgccc cgcactggga atggcccaga
     2161 accaccacca gccgagtgcc gaaccgctcg gccgcaaact cgacgctgcc gatctgagag
     2221 gcggcgacga tgtttccggc aacgcggatg acaaacaggt cgcccaggcc ccgttccgac
     2281 tcgcgggtac cggcagagat cgtcttcgac gggggacgag atttggttc
//
LOCUS       DATMCV010000683         3404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_170541, whole genome shotgun sequence.
ACCESSION   DATMCV010000683 DATMCV010000000
VERSION     DATMCV010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170541"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VNU03_15640"
     CDS             <1..309
                     /locus_tag="VNU03_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWP75955.1"
                     /translation="TLRPGMYADVKLHLERDHPPLIIPTSALIIQATGPRVAVLGAEQ
                     KVRFQAVELGRDYGATVEIVRGIEPGDQVITSPPDGLSESSVVRVAPTPAPAGPGKKS
                     "
     gene            complement(326..1354)
                     /locus_tag="VNU03_15645"
     CDS             complement(326..1354)
                     /locus_tag="VNU03_15645"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HWP75956.1"
                     /translation="MTPAAGDDNRLLEVRDLQKLFPIRRGFLRRVVGQVRAVDGVSFH
                     VERGETLALVGESGCGKTTTSRCVLRAVTPTAGQILFRTAEGPVLDVATLPRRGLRPL
                     RRQMQMIFQDPFSSLNPRRTLLDIVAEPLVANRVGTRAERVERVAELLRLVGLRPEYM
                     RRYPHAFSGGQRQRVGIARALALNPRLVVADEPVSALDVSVQAQILNLMLDLQAQLGL
                     TYLFVAHDLSVVKHVSDRVAVMYVGQIVELAETEALFTSPRHPYTEALLSAVPKPDPR
                     LRAQRIVLEGDVADAAHPPPGCYFHPRCGYAVERCRVETPRLEPVAPGHFVACHRARE
                     LTLRGIAP"
     gene            complement(1351..2373)
                     /locus_tag="VNU03_15650"
     CDS             complement(1351..2373)
                     /locus_tag="VNU03_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75957.1"
                     /translation="MTATPADAARPILEVRDLRTYFFQDEGTVKAVDGAGLSVYAGKT
                     LGIVGESGCGKSVTARSILRIVERPGRVVSGEILLREDGRETDLARLDAESREMRQVR
                     GGDIALIFQEPMTSFSPVHTVGNQMIETIRLHQALDKKAAREKAIEMFRLVGIPRPER
                     RVDEYAFQLSGGLRQRAMIALALSCAPRILIADEPTTALDVTTQAQILSLLTRLQQRE
                     GMAIILITHNLGVVAEMCDQVAVMYLGRVVEQGPVDAIFHAPRHPYTRALLRSIPSIH
                     APVRTRLPTIAGSIPHPYNRPPGCPFHPRCPDFMPGRCDRAEPALLTVAAGQQAACFL
                     YGDAQP"
     gene            complement(2375..3394)
                     /locus_tag="VNU03_15655"
     CDS             complement(2375..3394)
                     /locus_tag="VNU03_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020040278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP75958.1"
                     /translation="MLGTAVVLAFYAAVLFADFLAYADPGASEAQRGLLPPQRIHWTI
                     DGRWAPHVYGLAGKRDPTTFKRVYTPDPSQKVPVRFFAHGFEYRLLGVLPTTRHLIGV
                     EGARPEDTLFLLGTDEQGRDLWSRLMYGTRTSLTIGLVGVAFSLVLGVMLGGVSGLYG
                     GLADTVIQRVIEIVRSIPTIPLWMGLAAALPNDWTVMQVYFAITIIISLVGWTELARV
                     VRGRFLALREEDFVTAAVLAGSSQMRIIVHHLVPSFLSHIIAATTLAIPAMIISETSL
                     SFLGLGLRPPAISWGVLLQQAQNIQALAISPWLLVSAVPVIIVILALNFMGDGLRDAA
                     DPYGQ"
BASE COUNT          519 a         1193 c         1257 g          435 t
ORIGIN      
        1 acgctccggc ccgggatgta cgcggacgtg aagctccacc tcgagcgcga ccacccgccc
       61 ctgatcatcc cgaccagcgc cctgatcatc caggccaccg gcccgcgcgt cgcggtgctg
      121 ggggccgagc agaaggtccg cttccaggcg gtcgagctgg ggcgggacta cggggccacc
      181 gtcgagatcg tgcgggggat cgagcccggc gaccaggtga tcacctcgcc gccggacggg
      241 ctcagcgaga gcagcgtggt gcgggtcgcg cccaccccgg cgcccgccgg gcccggcaaa
      301 aaatcctgag ccgagaagtc ctgatctagg gcgcgatgcc gcgcagggtg agctcgcggg
      361 cgcggtggca ggccacgaag tggcccggcg ccaccggctc cagccgaggg gtctccaccc
      421 ggcagcgctc gaccgcgtag ccacagcgcg ggtggaagta gcagcccggc ggcggatgcg
      481 ccgcgtccgc cacgtcgccc tccagcacga tgcgctgcgc gcgcaggcgc gggtccggct
      541 tgggcaccgc ggagagcagg gcctcggtgt agggatgccg gggggaggtg aagagggcct
      601 cggtctccgc cagctccacg atctggccca cgtacatcac cgccacccgg tcgctcacgt
      661 gcttcaccac gctgagatcg tgggccacga agagataggt gaggccgagc tgggcctgca
      721 ggtcgagcat caggttgagg atctgggcct gcaccgagac gtcgagggcc gagaccggct
      781 cgtccgccac caccagccgc gggttgagcg cgagggcccg ggcgatgccg acgcgctggc
      841 gctggccccc gctgaaggcg tgcggatagc ggcgcatgta ctcgggccgc aggcccacca
      901 gccgcagcag ctcggccacc cgctccaccc gctcggcgcg ggtgcccacc cggttggcca
      961 cgagcggctc ggccacgatg tcgaggaggg tgcgccgcgg gttcagcgag gagaacggat
     1021 cctggaagat catctgcatc tgccggcgca gcggtcggag tccccgccgg ggcaaggtgg
     1081 ccacgtccag gaccggcccc tcggcggtgc ggaacaggat ctgaccggcg gtgggcgtga
     1141 ccgcccgcag cacgcagcgc gacgtcgtgg tcttgccgca gccgctctcg cccaccaggg
     1201 ccagcgtctc gccgcgctcg acgtggaagc tcaccccgtc caccgcccgc acctggccga
     1261 ccacccgccg caggaatccc cggcggatgg ggaagagctt ctggaggtcc cgcacctcga
     1321 gcagccggtt gtcgtcgccc gcggccggcg tcacggctgc gcgtctccgt agaggaagca
     1381 ggccgcctgc tgccccgcgg ccaccgtcag gagggccggc tccgcccgat cgcagcggcc
     1441 gggcatgaag tccgggcagc gcggatggaa cgggcatccc gggggccggt tgtaggggtg
     1501 cgggatcgac ccggcgatgg tgggaagccg ggtgcgcacc ggcgcgtgaa tgctcgggat
     1561 cgagcgcagg agcgcgcggg tgtacggatg ccggggcgcg tggaagatgg cgtcgaccgg
     1621 cccctgctcc accacccggc cgaggtacat caccgccacc tggtcgcaca tctcggcgac
     1681 cacgccgagg ttgtgggtga tcaggatgat ggccatgccc tcgcgctgct gcaggcgggt
     1741 gagcagcgag aggatctggg cctgggtcgt gacgtcgagc gcggtggtcg gctcgtccgc
     1801 gatcaggatg cgcggcgcgc aggagagcgc gagcgcgatc atggcgcgct ggcggaggcc
     1861 cccgctcagc tggaacgcgt actcgtcgac ccgccgctcc gggcgcggga tgccgaccag
     1921 gcggaacatc tcgatggcct tctcgcgggc cgccttcttg tcgagcgcct ggtggaggcg
     1981 gatcgtctcg atcatctggt tgcccaccgt gtgcaccggg ctgaacgagg tcatcggctc
     2041 ctggaagatg agcgcgatgt cgcctccgcg gacctgccgc atctcccggc tctccgcgtc
     2101 gaggcgggcg aggtcggtct cgcggccgtc ctcccgcagg aggatctccc cgctgaccac
     2161 ccggcccggc cgctcgacga tgcggaggat cgagcgcgcg gtcacgctct tgccgcagcc
     2221 gctctccccc acgatgccga gggtcttgcc cgcgtagacc gagagtcccg ccccgtccac
     2281 cgccttcacg gtgccctcgt cctggaagaa gtaggtccgc agatcgcgaa cctcgaggat
     2341 cggacgcgcg gcgtcggcgg gcgtggcggt cacgttactg gccgtaggga tcggcggcat
     2401 cccgcagccc gtcgcccatg aaattgagcg cgaggatcac gatgatgacc ggcaccgcgg
     2461 agaccaggag ccacggcgag atcgcgagag cctggatgtt ctgcgcctgc tgcagcagga
     2521 cgccccacga gatggcgggc gggcgcagcc ccaggcccag gaacgacagc gacgtctcgc
     2581 tgatgatcat ggccgggatg gccagcgtcg tcgccgcgat gatgtggctc aggaacgacg
     2641 gcaccaggtg gtggacgatg atgcgcatct ggctcgagcc cgcgagcacc gccgcggtga
     2701 cgaagtcctc ctcccgcagg gcgaggaagc ggccgcgcac cacccgggcc agctcggtcc
     2761 agcccaccag cgagatgatg atggtgatgg cgaagtagac ctgcatcacc gtccagtcgt
     2821 tgggcagggc ggccgcgagc cccatccaga gcgggatcgt cgggatcgag cgcacgatct
     2881 cgatcacccg ctggatgacg gtgtcggcga ggccgccgta gagcccggag accccgccca
     2941 gcatgacgcc cagcaccagg ctgaacgcga cgcccaccag gccgatggtg agggacgtgc
     3001 gcgtgccgta catgaggcga gaccagagat cgcgcccctg ctcgtcggtg cccaggagga
     3061 acagggtgtc ctccgggcgc gcgccctcca ccccgatcag gtgccgcgtg gtcggcagca
     3121 cccccagcag ccggtactcg aagccgtggg cgaagaagcg caccgggacc ttctggctcg
     3181 gatccggggt gtagacccgc ttgaacgtcg tcgggtcgcg cttgcccgcg aggccgtaga
     3241 cgtggggcgc ccagcgcccg tcgatcgtcc agtggatgcg ctggggcggc agcagcccgc
     3301 gctgcgcctc cgaggcgccg gggtccgcgt aggccaggaa gtccgcgaac agcaccgccg
     3361 cgtagaaggc caggaccacc gcggtgccca gcatggccag ccgg
//
LOCUS       DATMCV010000684         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_33576, whole genome shotgun sequence.
ACCESSION   DATMCV010000684 DATMCV010000000
VERSION     DATMCV010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33576"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VNU03_15660"
     CDS             <1..696
                     /locus_tag="VNU03_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP75959.1"
                     /translation="LGIGVSHKPMMEAALGLDMGRPLEVTREYVTVLRAALTGKVQHQ
                     GARYRVNWQSGLPRVPAAPPVLLGGLAPRMLELAGEIADGVVLWLAAPAYVREQAVPA
                     IRRGRERAGKALQGFEIVAAVPAALTADRAAAGLLFRTELTRYLALPFYRAMLEQSGF
                     GAEIAAYDRSPKPEAVPERLVGALGGIGDFRTVSAFVESYREAGVTLPAVRPIGFPDA
                     THYEPTVEAGATA"
     gene            693..1373
                     /locus_tag="VNU03_15665"
     CDS             693..1373
                     /locus_tag="VNU03_15665"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HWP75960.1"
                     /translation="MIAMHADLLLRVLAGTMLGAVIGYERRIHRRPAGLRTHLLVALA
                     ASVFMVVSTNFVYFQHYVRDDLVAVDPSRIAASVVTGVGFLGGGAILRTGVNVQGLTT
                     AAGLWLCAAIGLSAGAGMYVVSVFSTLLGVVALTLLRQLEHKEDDLVTRRVSLTLGDP
                     APPWPTIMASLRARHIAITPGEYERRLDEGSVEVTFQARVAGDDADDLIAALEAQPGV
                     RRIRVESA"
     gene            1403..1672
                     /locus_tag="VNU03_15670"
     CDS             1403..1672
                     /locus_tag="VNU03_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75961.1"
                     /translation="MDNPFVKHVALPALAPAALIGLYFTPVLLFGCVNRGLLAVAVAL
                     VSAIGAFVTVGIALRLRAQGKPSGLWILSTAILTSPLALLVGPLG"
     gene            1705..>2113
                     /locus_tag="VNU03_15675"
     CDS             1705..>2113
                     /locus_tag="VNU03_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75962.1"
                     /translation="MLVALTVGWELYAIVAVSDTGVGFLDVALVVPTACLLAIAWALP
                     WSGATRRRPWLLGLWTSFAVLGAALVVIYLSSQSPFNPLFRARFHASEPALARAARGL
                     AGGPGHAEGLRVGLFRAKYVTVMSPDEVRFLTAE"
BASE COUNT          231 a          791 c          758 g          333 t
ORIGIN      
        1 ctcggcatcg gggtgagcca caagcccatg atggaggccg ccctcgggct cgacatgggc
       61 cggcccctcg aggtcacgcg cgagtacgtg acggtgctcc gcgccgcgct caccggcaag
      121 gtgcagcacc agggcgcacg ctaccgggtc aactggcaga gcggcctgcc gcgggtgccc
      181 gccgcaccgc ccgtcctgct gggtgggctg gccccgcgca tgctggagct ggccggcgag
      241 atcgccgacg gcgtcgtgct gtggctggcc gcgcccgcgt acgtgcggga gcaggcggtg
      301 ccggccatcc ggcgcgggcg cgagcgggcg ggcaaggcgc tccagggctt cgagatcgtg
      361 gccgcggtgc cggccgcgct gaccgccgat cgcgccgccg ccggcctgct cttccgcacc
      421 gagctcacgc gctacctcgc gctgccgttc taccgggcca tgctcgagca gagcgggttc
      481 ggcgcggaga tcgccgccta cgaccggtcg ccgaagccgg aggcggtgcc cgagcggctc
      541 gtgggcgcgc tgggcggcat cggcgacttc cggaccgtct ccgccttcgt cgaatcgtac
      601 cgggaggccg gcgtcaccct ccccgcggtg cggcccatcg gcttccccga cgccacccac
      661 tacgagccca ccgtggaggc cggcgccacc gcatgatcgc catgcacgcg gatctgctgc
      721 tgcgcgtgct cgcggggacg atgctggggg cggtgatcgg ctacgagcgg cggatccatc
      781 gccggcccgc cggcctccgc acgcacctgc tggtggcgct agccgcctcc gtgttcatgg
      841 tcgtctcgac gaacttcgtc tacttccagc actacgtgcg cgacgacctg gtcgcggtgg
      901 atccctcccg aatcgcggcc tcggtggtga ccggggtcgg cttcctcggt ggcggtgcga
      961 tcctgcgcac cggggtgaac gtgcaggggc tcaccaccgc ggcgggcctc tggctctgcg
     1021 cggccatcgg gctctccgcc ggcgccggca tgtacgtggt gagcgtcttc tcgacgctgc
     1081 tcggggtggt cgcgctcacg ctgctgcggc agctcgagca caaggaggac gacctggtca
     1141 cccgccgggt ctcgctgacg ctgggtgacc cggcgccgcc ctggccgacc atcatggcgt
     1201 cgctgcgcgc ccgccacatc gcgatcacgc ccggcgagta cgagcggcgg ctcgacgagg
     1261 gctcggtgga ggtgacattc caggcccgcg tcgcgggcga cgacgccgac gacctgatcg
     1321 ccgcgctgga ggcccagccc ggcgtgcgcc gcatccgagt cgagtccgcc tgaaattcgt
     1381 cccgtgtcag actggagggg ccatggacaa ccccttcgtc aagcacgtgg ccctgccggc
     1441 cctcgccccg gcggccctga tcgggctcta cttcacgccg gtgctcctgt tcggctgcgt
     1501 gaaccgcggg ctcctcgcgg tggcggtcgc gctcgtctcg gccatcggcg ccttcgtcac
     1561 ggtcggcatc gcgctccgcc tccgggcgca gggcaagccc tcgggcctgt ggatcctctc
     1621 gaccgccatt ctgacctcgc ccctcgccct gctcgtgggc ccgctcggct gacgagcatc
     1681 gggattcgct cgatgtggag agcggtgctc gtcgcgctga ccgtcgggtg ggagctctac
     1741 gcgatcgtcg cggtgagcga caccggcgtg gggttcctcg acgtcgcgct ggtcgtcccc
     1801 accgcgtgtc tgctcgcgat tgcctgggca cttccgtgga gcggggcgac ccgccgccgg
     1861 ccctggctcc tcgggctctg gacctcgttc gcggtcctgg gcgccgccct ggtcgtcatc
     1921 tatctctcga gccagagccc gttcaacccg ctgttccgtg cccggttcca cgcgagcgag
     1981 cctgcgctag cccgggccgc ccgcggactc gccggcggcc cgggccacgc cgagggactg
     2041 cgggtcggcc tgttccgcgc gaagtacgtg acggtcatgt cgcccgacga ggtgcggttc
     2101 ctcaccgccg agt
//
LOCUS       DATMCV010000685         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_503260, whole genome shotgun sequence.
ACCESSION   DATMCV010000685 DATMCV010000000
VERSION     DATMCV010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503260"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..2418
                     /locus_tag="VNU03_15680"
     CDS             13..2418
                     /locus_tag="VNU03_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP75963.1"
                     /translation="MNNQTTSLTSIQKDFPFESRLSLGLLIRFWEDLAKVDSVPGEQA
                     RSLLARLREVPALTGPIDDITLLDEHGPLVDALMSAVFPAAFLEKAYMGALIPFTLRS
                     VYGTRAFENIMGPDGVLYGSLNLDMQGLKDFRLVNAYALALERLYGIEFPVDYPLILT
                     APEPVSGLDRHYKIQFDGRFVDVDRLLPIPPLSEDMRQRLQAQALDLEGLASLIPAGS
                     VRFSGFTVVKAADVTDQEVLSSIKRDLIDKESIVANARFEKLQAKLRTLFRRAELQLT
                     LAAVEGDRILMLNSGHQLQQGNGCIFASSVHAKTIDYAGSVFEKATRRGAPVFVEDLA
                     ALPHRSAKEEMILSKGMRSLVVAPLFYQRELIGMLSLGSPHPGDLNTLLVPRLQEVLP
                     LFSVAVKRSVDELHSRVESFIKEQFTAIHPVVEWRFRKTVLGAMERPGSDHGHGEMDP
                     IVFRDVHPLYAVSDIRGSSTHRAWAIQADLLKQLELAREVLRAAHDARALPILDQLTH
                     RIDGHVAEIEVTLRSGDELGVMNFLKADVEPLFDHLQTFGDTVRERIDAYRAAVDPGI
                     GLVYARRKEFDESVTTINETISSYIDLEEQAAQGMFPHYFEKQKTDGVDYTIYLGGAL
                     REDGIFDPLYLKNLRLWQLMVACGVALRAERLKDRLPVPLDITNLILIQHAPLAIRFR
                     FDEKRFDVDGAYNVRYEIIKKRIDKAIIRGTTERLTQPGKIALVYSHATEAAEWREYI
                     EYLQRLGYLTREVEELELDELQGAQGLRALRVTVDLANRELDPPSTAASLPRSEGERT
                     PAG"
     gene            2433..2651
                     /locus_tag="VNU03_15685"
     CDS             2433..2651
                     /locus_tag="VNU03_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP75964.1"
                     /translation="MHRLAVLLLALALLGGCATASRPASARADEASATPRRCSTADPD
                     RGAWFCVVGQFLYGAASFFQPVNELSMR"
     gene            complement(2658..>3128)
                     /locus_tag="VNU03_15690"
     CDS             complement(2658..>3128)
                     /locus_tag="VNU03_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP75965.1"
                     /translation="VNLRAVFFVLQAAVRRMLKQDLIPGSELRGKLIQTASIAAYRGG
                     NHLMTPYSATKAGVVSVTRSAAQALAGERITSNCVCPGAVETPMWEQIDREWGGLEGL
                     GQGETWKRRIRGIPLGRPERPEDVASVVAFLAGPDSDYMTGQALNVDGGLVMGS"
BASE COUNT          486 a         1126 c         1047 g          469 t
ORIGIN      
        1 agaataatga gcatgaacaa tcagacgacg tcgctgacga gcatccagaa ggacttcccg
       61 ttcgagagcc gcctgagcct cgggctcctg atcaggttct gggaggatct cgccaaggtc
      121 gacagcgtgc ccggggagca ggcgcgtagc ctgctggccc ggctgcgcga ggtgccggcg
      181 ctgaccggcc ccatcgacga catcaccctg ctcgacgagc acgggccgct ggtcgacgcc
      241 ctgatgagcg cggtcttccc cgccgccttc ctcgagaagg cctacatggg ggcgctgatc
      301 ccgttcaccc tgcggagcgt gtacggaacc cgggccttcg agaacatcat gggtccggac
      361 ggcgtgctct acggcagcct gaacctcgac atgcaggggc tcaaggactt ccggctcgtg
      421 aacgcgtacg cgctggcgct cgagcgcctg tacggcatcg agttcccggt ggactatccg
      481 ctgatcctca ccgcacccga gcccgtcagc ggactggacc gtcactacaa gatccagttc
      541 gacggccgct tcgtggacgt cgaccgcctg ctcccgatcc cgccgctctc cgaggacatg
      601 cgccagcgcc tccaggcgca ggccctggac ctggagggcc tcgcgtccct gatcccggcg
      661 ggcagcgtgc gcttctccgg cttcacggtg gtgaaggcgg cggacgtgac cgaccaggag
      721 gtgctgtcgt cgatcaagcg cgacctgatc gacaaggagt cgatcgtcgc caacgcgcgc
      781 ttcgagaagc tgcaggccaa gctccgcacc ctcttccggc gggccgagct gcaactgacc
      841 ctggccgcgg tggagggcga ccggatcctc atgctcaact ccggtcacca gctccagcag
      901 ggcaacggct gcatcttcgc gagctcggtg cacgccaaga ccatcgacta cgcgggctcg
      961 gtcttcgaga aggcgacccg gcggggcgcg ccggtcttcg tggaggacct cgccgcgctg
     1021 ccccatcgct cggccaagga agagatgatc ctgagcaagg gcatgcggag cctggtggtc
     1081 gcgcccctgt tctaccagcg cgagctgatc ggcatgctga gcctgggctc gccccacccg
     1141 ggggacctca acaccctgct ggtgccgcgc ctccaggagg tgctgccgct cttctcggtg
     1201 gcggtgaagc gcagcgtgga cgagctgcac tcgcgggtgg agtccttcat caaggagcag
     1261 ttcaccgcca tccatccggt ggtggagtgg cgcttccgca agacggtgct gggggcgatg
     1321 gagcggcccg gctctgatca cgggcacggt gagatggacc cgatcgtctt ccgcgacgtc
     1381 caccccctct acgcggtgtc ggacatccgc ggctcgtcca cccaccgcgc gtgggccatc
     1441 caggcggacc tgctcaagca gctcgagctc gcccgcgagg tgctgcgcgc cgcccacgac
     1501 gcgcgcgccc tgccgatcct cgaccagctc acccaccgta tcgacgggca cgtggccgag
     1561 atcgaggtga ccctgcgctc gggcgacgag ctcggggtga tgaacttcct gaaggccgat
     1621 gtggagccgc tcttcgacca cctgcagacc ttcggggaca cggtgcgcga gcgcatcgac
     1681 gcctaccggg ccgcggtgga tcccgggatc ggcctggtct acgcccggcg caaggagttc
     1741 gacgagagcg tcaccaccat caacgagacg atctcgtcct acatcgacct ggaggagcag
     1801 gccgcccagg ggatgttccc gcactacttc gagaagcaga agaccgacgg ggtcgactac
     1861 acgatctacc tgggcggggc cctccgcgag gacgggatct tcgacccgct ctacctgaag
     1921 aacctgcgcc tgtggcagct catggtcgcg tgcggggtgg ccctgcgggc cgagcggctg
     1981 aaggaccggc tgccggtgcc cctcgacatc acgaacctga tcctgatcca gcacgccccg
     2041 ctcgccattc gcttccgctt cgacgagaag cgcttcgacg tggacggcgc ctacaacgtg
     2101 cgctacgaga tcatcaagaa gcggatcgac aaggcgatca tccgcggcac caccgagcgg
     2161 ctgacccagc ccggcaagat cgccctggtc tactcgcacg ccaccgaggc cgcggagtgg
     2221 cgcgagtaca tcgagtacct ccagcgcctg ggctacctca cccgcgaggt cgaggaactg
     2281 gagctggacg agctgcaggg agcccagggc ctccgggccc tccgggtcac ggtggatctc
     2341 gccaaccggg agctcgaccc gccctcgacc gcggcctcgc tgcctcggag cgagggcgag
     2401 cgcacccccg ccgggtaagc cgggcgcgga cggtgcatcg gctcgccgtc ctgctgctcg
     2461 cgctggcgct gctgggcggc tgcgccaccg catcacgccc cgcgagcgcg cgggccgacg
     2521 aggcctccgc gacgccgcgc cgctgctcga ccgccgatcc cgaccgcggg gcgtggttct
     2581 gcgtggtcgg gcagttcctc tacggcgcgg cctcgttctt ccagccggtc aacgagctgt
     2641 cgatgcggtg acccggctca gctccccatc acgagcccgc cgtccacgtt gagggcctgc
     2701 ccggtcatgt agtcggaatc ggggccggcg aggaacgcca ccacgctcgc cacgtcctcc
     2761 ggacgctccg ggcggccgag cgggatgccg cggatgcgcc gcttccacgt ctcgccctgt
     2821 cccaggccct cgagcccgcc ccactcccgg tcgatctgct cccacatcgg ggtctcgacc
     2881 gccccggggc acacgcagtt ggaggtgatg cgctcgcccg cgagggcctg cgcggcggag
     2941 cgggtgacgc tcaccacccc ggccttggtc gccgagtagg gcgtcatcag gtggttcccc
     3001 ccgcggtagg cggcgatgga ggcggtctgg atcagcttgc cgcggagctc cgaccccggg
     3061 atcaggtcct gcttcagcat ccgccgcacc gccgcctgga ggacgaagaa gaccgcgcgc
     3121 aggttcac
//
LOCUS       DATMCV010000686         4023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_103602, whole genome shotgun sequence.
ACCESSION   DATMCV010000686 DATMCV010000000
VERSION     DATMCV010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103602"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1609)
                     /locus_tag="VNU03_15695"
     CDS             complement(284..1609)
                     /locus_tag="VNU03_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWP75966.1"
                     /translation="MARRPNFVFILADDLGYADLGCYGGRPAASGAPFATCSPSLDRM
                     AREGLRFTQGYANSSVCSPTRFALMTGRWQHRLRGGADEPIRSNARGSPTLGLPPTHP
                     TLPSLLRDAGYATALVGKWHLGYPPHFGPLKSGYQEFFGALSGGMDYFTHRDTAGNHD
                     LYENDSEVLRKGYLTDLISERAVEFIGRKHEEPFLLSVHYNAPHWPWLTRADGAESKR
                     IEKIYHFDGGSVVTYLTMIRQMDEGIGHILKTLKAARADRDTLVVFTSDNGGERFSDT
                     WPLVGKKMDLLEGGIRVPYIVRWPAKIRPGGSTEQVAITMDWVATFLDAAGVKPHPDY
                     PLAGVSLLPVLKNPSKIFERELYWKMLFRNQKAMRAGDWKYLSVEGDDFLFDLAKDQR
                     ERANFARREPERLAAMRARYLAWENVLPKHPDATYSVPATKADLLTPSS"
     gene            1837..2211
                     /locus_tag="VNU03_15700"
     CDS             1837..2211
                     /locus_tag="VNU03_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWP75967.1"
                     /translation="MQFLMLMIPKIYRGNKVDPGFLPDPKKLAEMGRFNEELGNAVTI
                     LSLNGLHPLNTGARVSFGKGKPTVTDGPFIETTEVLGGFWMVEASSKEELVKWAQRCP
                     ADEGDVIEIRQIFDEADFAIKK"
     gene            2356..>4023
                     /locus_tag="VNU03_15705"
     CDS             2356..>4023
                     /locus_tag="VNU03_15705"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP75968.1"
                     /translation="MPATGPEPRSYTPRHLVEKILASKSALEGERKPVTVLFADVARS
                     MELAERVDPEEWHRLLDRLFRILADGVHRYEGTINQYTGDGIMALFGAPIAHEDHAQR
                     ACAAALELARDLGALAEDVRRERGLEFAVRMGLNSGEVVVGRIGDDLRMDYTAQGHVV
                     GLAARVQQLAPPGGISVTEQTARLASGFFDLLDRGEQSLKGASVPVRVFDLRGPGPIR
                     SRFDRSRARGFSRFVGREREIALLEKALRDAEAGRPTVVVVTAEPGAGKSRLCHELVE
                     RSRDVPLHYARALSHGRMLPFHAIVELARGLFGLDQDASAPDIRSAVGRGLASSQLVD
                     PIALAFWLELLGAPDPAMAPSELEPEARRTRLFQSFCDLIQARARREPTLLWVEDLHW
                     LDPASEAALQMLTERLLAPESAGSRALLLATTRPEYRPAWSARVERLSLAPLAAQHSS
                     ALLDDWLGSDPALAPLSARIEASARGNPLFVEEIVRSLVERGVLRGERGAYTPAAPVE
                     EITLPETVQAVLASRIDRLSPRDKDVLQAAAVVGRDVPTELLRVVVDL"
BASE COUNT          629 a         1334 c         1401 g          659 t
ORIGIN      
        1 agggcgatcg tgatccggat ggcgtcggtc cgggagagcg tcatcgcggc caggacaccg
       61 aatgccgcga acggggtcgc gcaggcaaag acgaagctga gtgcgctgac cgcgagaacc
      121 agaagcgcca gccaggcctg gtgagcccac tccggatagg gggatcgcga atccataccc
      181 ttgttcctcc tcctgctcgg tggtaccgcg tggtggctcg acgcccgaat cgaactgaat
      241 ctatgccttt cgccatacca gggcaagagt acggccgatg cccctagctg gagggtgtga
      301 ggagatcggc cttggtggcc ggcactgagt acgtcgcgtc cggatgcttc ggcagcacgt
      361 tttcccaagc gaggtagcgc gcgcgcatcg cggcgaggcg ctccggctcg cgccgggcga
      421 agttcgcacg ctcgcgctga tccttcgcga gatcgaacag gaagtcgtcg ccctcgacgg
      481 ataggtactt ccagtcgccg gcgcgcatcg ccttctggtt gcggaagagc atcttccagt
      541 agagctcgcg ctcgaagatt ttcgacggat tcttcaacac cgggagcagg ctcacgccgg
      601 ccagcggata gtccgggtgc ggcttcacgc cggccgcatc gaggaatgtc gccacccagt
      661 ccatggtgat cgccacctgc tcggtggagc cgcccggcct gatcttcgcc ggccagcgca
      721 cgatgtaggg cacgcggatg ccgccttcga gcagatccat cttcttgccg accagcggcc
      781 aggtgtcgga gaagcgctcg ccgccgttgt cgctggtgaa gacgaccagc gtatcgcggt
      841 ccgccctcgc cgctttcagc gtcttgagga tgtgcccgat gccctcgtcc atctggcgga
      901 tcatggtgag gtaggtcaca accgagccgc cgtcgaagtg gtagatcttc tcgatgcgct
      961 tcgactccgc cccatccgcg cgcgtgagcc aaggccagtg cggcgcgttg tagtggaccg
     1021 acaggaggaa cggttcctcg tgcttgcgtc cgatgaactc gaccgcccgc tcggagatga
     1081 ggtccgtgag gtagcccttc cggagcacct cggagtcgtt ttcgtacagg tcgtggttgc
     1141 ccgcggtatc gcggtgagtg aagtagtcca tgccgcccga gagcgcgccg aagaactcct
     1201 ggtacccgct cttcagcggc ccgaagtgcg gcggatagcc gagatgccac ttgcccacga
     1261 gtgcggtggc gtaaccggcg tcgcgcagga gcgacggcag cgtcgggtgc gtcggcggca
     1321 ggcccagggt cggactaccc cgcgcgttgc tccggatggg ctcgtcggcg ccgccgcgca
     1381 ggcgatgctg ccagcggccg gtcatcagcg cgaagcgggt cggcgaacag acggatgagt
     1441 tggcgtagcc ctgtgtgaat cggagcccct cgcgtgccat gcggtcgagc gacggcgagc
     1501 acgtggcgaa cggcgcgccg ctcgcggccg gccggccccc atagcacccc agatcggcgt
     1561 agccgaggtc gtccgcgaga atgaagacga agttggggcg gcgggccatg cctcgcgagg
     1621 atagcctagg atcctgagga aggcgatccc cacgagggcc gaagcgatgc cggagtcgac
     1681 ccagtcccgt gtgattggtc tggtcaaact ggccggaatc ggctcttgag ctgatccttt
     1741 ccgtccctta cggtgaagag cagtgaggcg catgtccgag caccgcgcga tcatgccgcg
     1801 actgtcgcag cgcttcacaa gagaaggaga agacccatgc aattcctgat gctgatgatc
     1861 cccaagattt accgaggcaa caaggtggat cccggctttc tgcccgaccc aaagaaactt
     1921 gccgagatgg gccgcttcaa cgaggagctg ggcaacgcgg tcacgatcct ctcgttgaac
     1981 ggccttcatc cgctcaacac cggcgcgcgg gtttcgttcg gcaaagggaa gccgacggtg
     2041 accgatgggc cgttcatcga gacgacagaa gtgctgggcg ggttttggat ggtggaagcc
     2101 agctcgaagg aagagctcgt gaaatgggcg cagcggtgtc ccgccgacga aggcgacgtg
     2161 atcgagatcc gtcagatctt tgacgaggcg gacttcgcca tcaagaaata ggaggcttac
     2221 ggagggggcg accgcctccc gaggtcgttc cggacggacg gggctaagat gggtggcgcg
     2281 atgagctgcc catcctgtgg acacgagaac cccgccgggg cgaggttctg caacgactgc
     2341 ggcgcgcgtc tggcagtgcc ggcgaccggt ccggagccgc gctcctacac gccgcgccac
     2401 ctcgtcgaga agatcctcgc ctccaagagc gcgctggagg gcgagcgcaa gcccgtcacc
     2461 gtccttttcg ccgacgtcgc gcgctcgatg gaactggccg agcgcgtcga tccggaggag
     2521 tggcatcggc tcctcgatcg cctgttccgg atcctcgcgg acggcgtcca ccgctacgag
     2581 gggacgatca accagtacac cggcgacggc atcatggcgc tcttcggggc cccgatcgcc
     2641 cacgaggacc acgcccagcg cgcctgcgcg gccgcgctcg agctggcgcg cgacctcggg
     2701 gcgctcgccg aggacgtgcg gcgcgagcgc gggctcgagt tcgcggtgcg gatgggcctg
     2761 aactcgggcg aggtcgtcgt cgggcgaatc ggcgacgacc tgcgcatgga ctacaccgcg
     2821 cagggccacg tcgtcgggct cgccgcccgc gtgcagcagc tggcgccgcc gggcggcatc
     2881 agcgtgaccg agcagaccgc gcggctcgcg tcggggttct tcgacctcct cgatcgcgga
     2941 gagcagagcc tcaagggcgc gagcgttccg gtgcgggtgt tcgacctgcg gggtccgggt
     3001 ccgatccgga gtcgcttcga ccgttcacgc gcccgcggct tctcgcgctt cgtcggccgg
     3061 gagcgcgaga tcgcgctgct cgaaaaggct ctgcgcgacg cggaggccgg tcggccgacg
     3121 gtcgtagtgg tcaccgcgga gccgggcgcg ggaaagagcc gcctgtgtca cgagctcgtc
     3181 gagcgctccc gtgacgtgcc gctccactat gcccgtgcgc tctcccacgg acgcatgctc
     3241 ccctttcacg ccatcgtcga gctggcccgc ggtctcttcg gcctagacca ggacgcgtcg
     3301 gcgccggaca tccgaagtgc cgtggggcgg ggtctggcga gctctcagct cgtcgacccg
     3361 atcgcgctgg ccttctggct cgagcttctc ggagcgcccg atcccgcgat ggccccttcg
     3421 gagctggagc ccgaggcgcg ccgtacgcgt ctgtttcagt cattctgcga tctgatccag
     3481 gcccgcgctc ggcgcgagcc cacgttgctc tgggtcgagg atctgcactg gctcgacccg
     3541 gcgagcgaag ctgcgcttca gatgctcacc gagcgccttc tggctccgga gtcggccggc
     3601 agccgggcgc tgctgctcgc gaccacgcga cccgagtatc ggcccgcgtg gtcggcacgg
     3661 gtcgagcggc tgtctctggc gcccctcgcc gcccagcact cctcggcgct gctcgacgac
     3721 tggctcgggt ccgaccccgc gcttgcgccg ctctcagccc ggatcgaggc cagtgcccgc
     3781 gggaatcctc tcttcgtcga ggagatcgtt cgctcgctcg tcgaacgggg tgtgctgcgc
     3841 ggcgaacgcg gcgcgtacac gccggccgcc ccggtcgagg agatcacact ccccgagacc
     3901 gtccaggctg tcctcgcctc gcggatcgat cgactctcgc cgcgcgacaa ggacgtgctc
     3961 caggccgcgg ccgtggtcgg ccgagatgtg ccgacggagc tgctgcgcgt cgtggtcgat
     4021 ctg
//
LOCUS       DATMCV010000687         2222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_303566, whole genome shotgun sequence.
ACCESSION   DATMCV010000687 DATMCV010000000
VERSION     DATMCV010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303566"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VNU03_15710"
     CDS             complement(<1..672)
                     /locus_tag="VNU03_15710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75969.1"
                     /translation="MVATLAALLYPIPFERSGQIYYETVGFLVLLNAMLGIGWNIIGG
                     WAGQFDFGPSIFFAIGAYTAALLSIHFGWNAWLALAGAVAVAVVICAALTYPVTRLRG
                     HYFAIATVAMWMIAQPIGATWSLINGSQGLFIPVAAGGSFVHGMLTMEFPGRGKGLGF
                     YYLSLLLFAVTLLGARAVERSKLGFYFRAVRDDQDGAEAIGIDSRLYKVIARCLMAAI
                     FAVGGT"
     gene            complement(713..1585)
                     /locus_tag="VNU03_15715"
     CDS             complement(713..1585)
                     /locus_tag="VNU03_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP75970.1"
                     /translation="MTIFLQAVADGLQMGLLYALVAVGLAIIWGLMEMINFAHGEFMM
                     LGMFGAWGLSVSLGWDPLVTMLPLAVVMYGLGVLVYRLLMQKVQRGEVFTQIFATVGL
                     FFFIQNGVVALFTSDYRFINKSVLTGLSGANLSLGGVRVGVPLLVGGLIALGLFLMLY
                     LLIDRTDFGLALQATAEDRDAAMLVGIRPQRMYAVAWGLGTALVAVAGVILANFFSVY
                     PQAGLSFTVLAYAIVALGGFGSILGTLLAALLVGVVQSVTAAYLPPAFKDAFVFGSYI
                     LIGLFRPQGLFGRF"
     gene            complement(1595..>2222)
                     /locus_tag="VNU03_15720"
     CDS             complement(1595..>2222)
                     /locus_tag="VNU03_15720"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP75971.1"
                     /translation="TTLASEVQRLQAANPDILFAVQYPAETTVFQADMKRANYMPKVI
                     ATSNGAYSDQTWLTAQKPTAGAAGWLGRDPTAIDLASKKASWKKVNEIYKKYSRGQDM
                     GELAMREITGMLWMADTINRAKSVEPAALAKAANETDMKPDMMIIDYKGIKFDKNRQN
                     ELATGVVTQVGWDDEKHTIWPWDLAAQAGFKPIYPSPSWQERETRSKK"
BASE COUNT          382 a          722 c          787 g          331 t
ORIGIN      
        1 cgtacccccg accgcgaaga tcgcggccat gaggcagcgg gcgatcacct tgtagagtcg
       61 gctgtcgatg ccgatggcct cggcgccgtc ctggtcgtcg cgcaccgcgc ggaagtagaa
      121 gccgagcttc gagcgctcca ctgcgcgcgc gccgagcagc gtgaccgcga agagcaggag
      181 cgacagatag tagaagccga gccctttgcc gcgcccgggg aattccatcg tgagcatgcc
      241 gtgcacgaag ctgccgccgg cggccaccgg gatgaagagg ccctggctgc cgttgatgag
      301 cgaccaggtg gccccgatgg gctgcgcgat catccacatc gccacggtgg cgatcgcgaa
      361 gtagtggccg cgcagccggg tgaccgggta ggtgagggcc gcgcagatga ccaccgccac
      421 cgccaccgcg cccgccagcg cgagccaggc gttccagccg aagtggatcg acaggagcgc
      481 ggcggtgtag gcgccgatcg cgaagaagat gctcggcccg aagtcgaatt ggccggccca
      541 gccgccgatg atgttccagc cgatgccgag catggcgttg agcaggacca ggaagcccac
      601 cgtctcgtag tagatctggc cgctgcgctc gaaggggatc gggtagagca gggccgcgag
      661 ggtggcgacc acccagacca cctgctgcgc gcggccggcg cgggccggcg agctagaagc
      721 ggccaaatag cccctggggc cggaacaggc cgatcaggat gtaggacccg aagacgaagg
      781 cgtccttgaa ggcgggcggc aggtaggccg cggtcacgct ctgcacgacc cccaccagga
      841 gcgcggcgag cagggtgccc aggatggagc cgaagccgcc cagggccacg atcgcgtagg
      901 cgagcacggt gaaggagagg cccgcctgcg gatacaccga gaagaagttg gccaggatga
      961 cgccggccac cgcgaccagc gcggtgccga ggccccaggc caccgcgtac atgcgctggg
     1021 gccggatgcc gaccagcatg gccgcgtcgc ggtcctccgc ggtggcctgg agcgcgaggc
     1081 cgaagtcggt gcggtcgatc agtagataaa gcatcaggaa caggccgagc gcgatcaggc
     1141 cgcccaccag gaggggaacg ccgacgcgta cccctcccag cgagaggttg gccccgctga
     1201 gcccggtgag cacgctcttg ttgatgaagc ggtagtcgct ggtgaagagc gccaccaccc
     1261 cgttctggat gaagaagaag aggccgacgg tggcgaagat ctgggtgaag acctccccgc
     1321 gctggacctt ctgcatgagc aggcgataca ccagcacgcc gaggccgtac atgacgaccg
     1381 cgagggggag catggtgacg agcgggtccc agccgagcga cacgctcagt ccccacgccc
     1441 cgaacatccc gagcatcatg aactcgccgt gggcgaagtt gatcatctcc atcaggcccc
     1501 agatgatggc gagccccacc gcgacgagcg cgtagagcag ccccatctgg agcccgtcgg
     1561 ccaccgcctg gaggaagatg gtcacggtcg gcggttactt cttgctccgg gtctcccgct
     1621 cctgccagct cggggacggg tagattggct tgaagccggc ctgggccgcg agatcccacg
     1681 gccagatggt gtgcttctcg tcgtcccagc cgacctgggt caccacgccg gtggccagct
     1741 cgttctggcg gttcttgtcg aacttgatgc ccttgtagtc gatgatcatc atgtcgggct
     1801 tcatgtcggt ctcgttggcc gccttggcca gcgcggccgg ctccaccgac ttggcgcggt
     1861 tgatggtgtc ggccatccag agcatgccgg tgatctcgcg catcgccagc tcgcccatgt
     1921 cctgcccgcg gctgtacttc ttgtagatct cgttgacctt cttccacgac gccttcttgc
     1981 tcgcgaggtc gatggcggtc ggatcgcggc cgagccagcc cgccgcgccc gcggtcggct
     2041 tctgggcggt cagccaggtc tggtcggagt aggcgccgtt gctggtggcg atcaccttgg
     2101 gcatgtagtt ggcgcgcttc atgtcggcct ggaacacggt ggtctccgcc gggtactgca
     2161 ccgcgaagag gatgtccgga ttcgcggcct gcagccgctg cacctcggag gcgagcgtgg
     2221 tg
//
LOCUS       DATMCV010000688         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_303570, whole genome shotgun sequence.
ACCESSION   DATMCV010000688 DATMCV010000000
VERSION     DATMCV010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303570"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VNU03_15725"
     CDS             <1..529
                     /locus_tag="VNU03_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWP75972.1"
                     /translation="SNIYYTPARAQAADFVNYATTGTAGIVKKGNPKGIKTIDDVCGK
                     RAAPILGTVEDKGFQDASAKCVAAGKPTIEITPYPNAPATSRAIENDRADLSMYDVVL
                     VDQVVRSNPDKFERAYYFRSNIKVGVAVKKGNEELVTAVKDAITELQANGTQKALMQK
                     NGIDPTLAIPVEIGR"
     gene            569..2170
                     /locus_tag="VNU03_15730"
     CDS             569..2170
                     /locus_tag="VNU03_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HWP75973.1"
                     /translation="MEAFLQYLTFPFLLEGMWLTLRIFLASMAGGLALGLLIALMRLS
                     PYRLLAGLAWTYVWLIRGTPVLLQLVFLFDALPFIGIVMPPVTTAIIGFSLNEAAFAG
                     EIIRGGILSVSRNQTLAAASLGMSSLLTLRRVVLPQAMRAILPAVGNDAISVLKGTSL
                     ASVIAVNELTLRSQQIVAQNFKFFPVFAAAGVMYLTVTTLITGIQFLLERRVSPDVDR
                     SQKPRSETALGRLFGFRWRLPEGKPAPAAGAGTSVHAAAPAARVDLVELVGGRAARTG
                     ADAAGPFVRCADVWKSYGHRAILQGVNFTVHRGDVVTLMGPSGSGKSTLLRLIAHLEP
                     MDRGEITVDGAHVGYERVDGVLRPSRHVARERARARIGMVFQQFNLFEHLTALENICE
                     APVRVHGERPELARARATTLLGSVGLAHHADHLPSRLSGGQQQRVAIARALAISPRVM
                     LFDEPTSALDPELVSEVLSVIRRLAEAGMTMIVVTHELRFAREVADRIVFMADGRIVE
                     EGTPEQVLEHPREARTQQFLKLVQD"
BASE COUNT          323 a          818 c          826 g          323 t
ORIGIN      
        1 gtcgaacatc tactacacgc cggcccgggc ccaggccgcc gacttcgtga actacgcgac
       61 caccggcacg gccgggatcg tcaagaaggg caacccgaag ggtatcaaga ccatcgacga
      121 cgtctgcggc aagcgggccg cgccgatcct cggcaccgtc gaggacaagg gcttccagga
      181 cgcgagcgcc aagtgcgtgg ccgcgggcaa gcccaccatc gagatcaccc cctatcccaa
      241 cgcgcccgcg acctcgcgcg ccatcgagaa cgaccgcgcc gatctctcga tgtacgacgt
      301 agtgctggtc gaccaggtgg tgcggagcaa cccggacaag ttcgagcgcg cgtactactt
      361 ccgcagcaac atcaaggtcg gggtggcggt gaagaagggc aacgaggagc tggtgaccgc
      421 ggtcaaggac gcgatcaccg agctgcaggc caacggcacc cagaaggcgc tgatgcagaa
      481 gaacgggatc gatccgacgc tggcgatccc ggtcgagatc ggccgctagc gcggaggcgc
      541 cggccggccg agcgcgagct ccggggacat ggaggcgttc ctccagtacc tgaccttccc
      601 cttcctgctg gaggggatgt ggctcacgct gcggatcttc ctcgcctcga tggcgggggg
      661 gctcgcgctc ggtctgctga tcgcgctgat gcgcctgtcc ccctaccggc tcctcgcggg
      721 gctcgcctgg acctacgtgt ggctgatccg gggcacgccg gtcctgctcc agctggtgtt
      781 cctgttcgac gccctgccgt tcatcggcat cgtgatgcct ccggtgacca ccgcgatcat
      841 cggcttctcg ctcaacgagg ccgccttcgc gggcgagatc atccggggcg gcatcctgtc
      901 ggtcagccgc aaccagaccc tggccgccgc ctccctcggg atgagctccc tgctcaccct
      961 gcggcgcgtc gtgctccccc aggcgatgcg cgcgatcctg cccgcggtgg gcaacgacgc
     1021 gatcagcgtg ctcaagggca cctcgctcgc ctcggtcatc gcggtcaacg agctgaccct
     1081 ccgcagccag cagatcgtgg cccagaactt caagttcttc ccggtcttcg cggcggccgg
     1141 cgtgatgtac ctgaccgtca ccaccctgat caccgggatc cagttcctcc tggagcggcg
     1201 ggtgagcccc gacgtggacc ggagccagaa gccgcggagc gagaccgcgc tgggccggct
     1261 cttcggcttc cgctggcggc tcccggaggg caagcccgcg cccgcggcgg gcgcggggac
     1321 gtccgtccac gcggcggcgc cggccgcgcg cgtcgatctg gtcgagctgg tgggggggcg
     1381 ggccgcgcgc accggcgccg acgccgcggg gccgttcgtg cgctgcgccg acgtctggaa
     1441 gtcgtacggg caccgggcca tcctgcaggg ggtgaacttc acggtgcatc gcggcgacgt
     1501 ggtcaccctg atggggccga gcggctcggg caagagcacg ctgctccggc tcatcgccca
     1561 cctcgagccg atggaccgcg gcgagatcac ggtggacggc gcgcacgtgg gctacgagcg
     1621 cgtggacggc gtgctccggc cctcacgcca cgtcgcgcgc gagcgggccc gcgcgcggat
     1681 cggaatggtc ttccagcagt tcaacctctt cgagcatctg accgcgctcg agaacatctg
     1741 cgaggcgccg gtgcgggtgc acggggagcg tccggagctg gcgcgcgcgc gggccaccac
     1801 gctgctcggg agcgtggggc tcgcccacca cgccgatcac ctgccctcgc ggctctccgg
     1861 tgggcagcag cagcgggttg ccatcgcgcg ggcgctcgcg atctcgccgc gggtcatgct
     1921 cttcgacgag ccgacctccg cgctcgaccc cgagctggtg tcggaggtgc tctcggtgat
     1981 ccgacggctc gcggaggcgg gcatgaccat gatcgtggtg acccacgagc tgcgcttcgc
     2041 ccgcgaggtg gccgaccgca tcgtcttcat ggccgacggc cgcatcgtcg aggagggcac
     2101 gcccgagcag gtcctcgagc acccgcgcga ggcccgcacc cagcagttcc tgaagctcgt
     2161 ccaggactag cggggcccct gccgccgccg ggccggtcag cgggccggcg gtggaggcgg
     2221 gggtcccggg ggcgcgggag gcgggggtgg cgccggcggt ggcccggccg gcggcgccgg
     2281 gatccgcgcg
//
LOCUS       DATMCV010000689         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_905037, whole genome shotgun sequence.
ACCESSION   DATMCV010000689 DATMCV010000000
VERSION     DATMCV010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905037"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="VNU03_15735"
     CDS             complement(<1..1592)
                     /locus_tag="VNU03_15735"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HWP75974.1"
                     /translation="MSERFFILDGPGFLFRAYHALPFLSTSRGVPSHAVFGMSTMLWK
                     LLREDTPDYFAVAWDPPGPTFREEKFAAYKETRAPTPDDLRSQISYVKTLFEALHLPV
                     LEVPGFEADDVLGTVVERTRDLPIELVLVTSDKDMLQLVSPRVRVFSTTGRGGDRVVF
                     DEAAVKAKWGVEPAQIPDILALMGDSIDNIPGVPGVGEKTAAKLIGQFGGVERLYENL
                     SLVPGKLRETLAANRKQALLSRELATVSTRVPIAVDLETFRRREPDWDKLRALWTEME
                     FHTLLRQVPAAPAAEAAGGDAVTLADAAALAQYLGRVPAGDPLAIETVGEGGPPDPTI
                     TALGLYHPAAGAARVAVASPADEQGRALVAALGGRTLIGHDAKALAEWWLARGGALPP
                     GEDTAVAAYLLNPARTNYKLEEVCAELLGEGPGIAPPGTRGKWIWDLWAMAPRALAEV
                     GLASLYEDIERPLIGVLAEMERHGIRIDQVRLGEFSRELEVHLERTTREIFGLAGEEF
                     NIGSPKQLAYILFEKLKLPPVRR"
     gene            1664..>2205
                     /locus_tag="VNU03_15740"
     CDS             1664..>2205
                     /locus_tag="VNU03_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWP75975.1"
                     /translation="MMVRDGDAAVTGDRFLVEGGHPLRGTVTPAGNKNEALPVLAACL
                     LVRGEVVVDNVPRIRDVTALLEGLRHLGVRVAVDEAGTVRLDASRLETRDPDPAVMSQ
                     LRGSVLLAPGLLARTGRAALPAPGGDRIGRRRVDTHLLALGTLGARVEAGPALRLTLE
                     GRFRGAEVFLDEMSVTATENT"
BASE COUNT          295 a          822 c          794 g          294 t
ORIGIN      
        1 cgcctcaccg gcggcagctt gagcttctcg aacaggatgt aggcgagctg cttcggcgag
       61 ccgatgttga actcctcgcc cgcgagcccg aagatctctc gggtcgtgcg ctcgaggtgc
      121 acctcgagct cgcgggagaa ctcgccgagc cggacctggt cgatccggat gccgtggcgc
      181 tccatctcgg ccagcacccc gatcaggggc cgctcgatgt cctcgtagag gctcgcgagg
      241 ccgacctccg cgagcgcgcg gggcgccatc gcccacagat cccagatcca cttcccgcgc
      301 gtgccgggcg gcgcgatccc cggcccctcg cccagcagct ccgcgcacac ctcctcgagc
      361 ttgtagttgg tgcgcgcggg gttgagcagg tacgcggcga ccgcggtgtc ctcgccgggc
      421 ggcagcgcgc cgccgcgggc cagccaccac tcggcgagcg ccttcgcgtc atgaccgatc
      481 agggtccggc cgccgagcgc ggccaccagg gcgcggccct gctcgtccgc gggcgaggcc
      541 accgcgaccc gggccgcccc cgccgccggg tggtagagcc cgagcgcggt gatggtggga
      601 tcgggcggcc cgccctcgcc gacggtctcg atcgcgagcg gatcgccggc cggcacccgg
      661 ccgagatact gagcgagcgc cgccgcgtcg gcgagggtga ccgcgtcccc cccggccgcc
      721 tccgccgccg gggccgccgg cacctggcgg agcagggtat ggaactccat ctcggtccag
      781 agcgcgcgca gcttgtccca gtccggctcg cgccgccgga acgtctccag atcgaccgcg
      841 atcggcacgc gggtgctgac ggtggccagc tcgcgtgaga gcagcgcctg cttgcgattg
      901 gcggccagcg tctcgcgcag cttgcccggc accagcgaga ggttctcgta gaggcgctcc
      961 accccgccga actggccgat cagcttggcc gccgtcttct cccccacccc gggcaccccg
     1021 gggatgttgt cgatggagtc gcccatgagg gccaggatgt cggggatctg cgcgggctcg
     1081 acgccccact tggccttcac cgccgcctcg tcgaagacca cccggtcccc gcctcggccg
     1141 gtggtggaga agacgcgcac gcgcgggctc accagctgga gcatgtcctt gtcggaggtg
     1201 accaggacca gctcgatcgg caggtcgcga gtgcgctcga ccacggtgcc cagcacgtcg
     1261 tcggcctcga agcccggcac ctccagcacc ggcaggtgca gggcctcgaa gagcgtcttg
     1321 acgtacgaga tctggctgcg gaggtcgtcc ggcgtcgggg cccgcgtctc cttgtaggcc
     1381 gcgaacttct cctcccggaa ggtcggcccg ggcgggtccc aggccaccgc gaagtagtcc
     1441 ggggtgtcct cgcgcaggag cttccacagc atggtcgaca tgccgaagac cgcgtggctc
     1501 ggcacccctc gcgaggtgga gaggaacgga agcgcgtgat aggcgcggaa gaggaagccg
     1561 gggccgtcca ggatgaagaa ccgctcgctc atgcgggcag atgatacacc ggcggcgccg
     1621 gccccgctcc cgcgccctat ccggtcaaaa aatcgggtcg gttatgatgg tccgcgatgg
     1681 agacgcggcg gtgaccggcg atcgattcct ggtggagggc ggccatcccc tgcggggcac
     1741 ggtcacgccg gccggcaaca agaacgaggc cctgcccgtc ctcgcggcct gcctgctcgt
     1801 gcggggggag gtcgtcgtcg acaacgtccc gcgcatccgc gacgtgaccg cgctgctcga
     1861 agggctccgc cacctggggg tgcgcgtcgc cgtcgacgag gcgggcaccg tgcgcctcga
     1921 cgcgagccgg ctcgagaccc gcgaccccga cccggccgtg atgtcccagc tccgcggctc
     1981 ggtgctgctg gcgcccggcc tgctcgcgcg aaccggccgg gcggcgctcc cggcgcccgg
     2041 cggcgaccgg atcgggcggc gccgcgtcga cacccacctg ctcgcgctcg gcacgctggg
     2101 ggcccgggtc gaagcggggc ccgcgctgcg cctcaccctc gagggccgct tccggggcgc
     2161 cgaggtcttc ctcgacgaga tgagcgtgac cgccaccgag aacac
//
LOCUS       DATMCV010000690         2688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_34073, whole genome shotgun sequence.
ACCESSION   DATMCV010000690 DATMCV010000000
VERSION     DATMCV010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34073"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VNU03_15745"
     CDS             complement(<1..1205)
                     /locus_tag="VNU03_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP75976.1"
                     /translation="MPDLTTLPRLLQRNARELGARPAMREKDRGIWQTWDWRQYHDQV
                     RDLALGLASLGFRRGDRLSVIGDNRPRLYWAQVAAQSLGGVSVPVYQDSIARELAYVW
                     NHAEVSVIVAEDQEQVDKVAALRDELPALRLVVYDDPRGMTGYRHDWLKSFEDVQEAG
                     RKFGVEHPGFFENQIELGRAEDVAVISYTSGTTGNPKGAMITHANAIGVAESFRQASQ
                     LVPEDTSLAYLPMAWAGDSVYTLFASLVAGFCANCPESPETVQRDLRELGPSTLLAPP
                     RIWENMLTAVLVRAADATPLKRRTFDYFRAAAERAEILRTEGKPMPARLRLATALGEF
                     FVYGPVRDQLGLRRTRWALTGGAPLGPDTFRFFRSIGVNLKQVYGSTETTGLVSLQPD
                     REANPTTAGR"
     gene            complement(1198..1992)
                     /locus_tag="VNU03_15750"
     CDS             complement(1198..1992)
                     /locus_tag="VNU03_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75977.1"
                     /translation="MSAQLEIAEVSKSFGGVLAVSRVSLEVRRGEILSVIGPNGAGKT
                     TLLNMISGFYHPDSGRIALEGSDVTRDSPSHLAELGIARTFQNIALFRGMTVLDNLML
                     GRHVRMKSGVLASFVYWGFAQREEVVHRRRVEDIIDFLRIQDLRRRPCGSLAYGLQKR
                     VELGRALALDPKILLLDEPMGGCNHEEKEDMARFVLDVNHEWGTTIILIEHDMGVVMD
                     ISDRVAVLDMGVKIAEGTPDEVRTNPQVIKAYLGDAKGSSNGGQNA"
     gene            complement(2003..>2688)
                     /locus_tag="VNU03_15755"
     CDS             complement(2003..>2688)
                     /locus_tag="VNU03_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP75978.1"
                     /translation="GAGKSTTLKAVSGLLRTERGEVTKGGIELGGEAIHRRAASEIVR
                     RGVVQVMEGRHVFEHLTVEENLLTGGYTRRNGHSLKSDLERVYEYFPRLRDRRSLDAG
                     YISGGEQQMLAIGRALMAHPRLMLLDEPSMGLAPMLVQEIFEIVGRLNREERVAVLLA
                     EQNANMALRFAQYVYVMENGRVVLDGDVKAVSENEDIKEFYLGLSGVGQRKSYRDVKN
                     YRRRKRWLS"
BASE COUNT          408 a          952 c          891 g          437 t
ORIGIN      
        1 cgcccggcgg tggtgggatt cgcttcccgg tcgggctgca gcgagacgag cccggtcgtc
       61 tcggtggatc cgtacacctg cttgaggttc actccgatcg agcggaagaa gcggaaggta
      121 tcgggaccga gaggggcccc gccggtcagc gcccacctgg tgcgccggag gcccagctgg
      181 tcgcgcaccg gcccgtacac gaagaactcg ccaagagcgg tggccagccg caggcgcgcc
      241 ggcatcggct tgccctccgt tctcaggatc tcggctcgct ccgccgccgc gcggaagtag
      301 tcgaaggtcc ggcgcttgag cggcgtcgcg tcggccgccc gcaccagcac cgcggtgagc
      361 atgttctccc agatccgcgg cggggcgagg agcgtggagg gccccagctc ccgcaggtcg
      421 cgctgcaccg tctccgggct ctcggggcag ttggcgcaga agccggccac caggctggcg
      481 aagagcgtgt agaccgagtc ccccgcccag gccatgggca gatacgccag cgaggtgtcc
      541 tccgggacga gctgcgacgc ctgccgaaag ctctccgcca cgccgatcgc gttggcgtgc
      601 gtgatcatcg cgcccttggg attgccggtg gtgccggagg tgtaggagat gacggccacg
      661 tcctcggcgc ggcccagctc gatctggttc tcgaagaagc ccggatgctc gacaccgaac
      721 ttccggccgg cctcctgcac gtcttcgaag gacttgagcc agtcatgccg gtagccggtc
      781 attcctctcg ggtcgtcgta gacgaccagg cggagggccg gcagctcgtc acgcagggcc
      841 gcgaccttgt cgacctgctc ctggtcctcg gccacgatca cgctcacctc cgcgtggttc
      901 cagacgtagg ccagctccct ggcgatcgag tcctggtaga cggggaccga gacgccgccc
      961 aggctctgcg cggccacctg ggcccagtac aggcgcggcc ggttgtcccc gatgacggaa
     1021 agccgatcgc cgcgacggaa gccgagggac gccaggccga gcgcgagatc ccgcacctgg
     1081 tcgtggtact gacgccagtc ccaggtctgc cagataccgc gatccttctc acgcatcgcc
     1141 ggtcgggcgc cgagctcgcg cgcgttgcgc tgcagcaagc ggggaagcgt cgtcagatca
     1201 ggcattctgg cctccattgc tgctcccctt ggcgtcgccc aggtacgcct tgatcacctg
     1261 cggattcgtc cgcacctcgt cgggggtgcc ctcggcgatc ttcacgccca tgtccagcac
     1321 cgccacccgg tccgagatgt ccatgaccac ccccatgtcg tgctcgatca ggatgatggt
     1381 ggtgccccac tcgtggttga catccaggac gaaccgggcc atgtcctcct tctcttcgtg
     1441 gttgcagccg cccatcggct cgtcgaggag gaggatcttc gggtcgaggg cgagggcgcg
     1501 gcccagctcc acgcgcttct gcaggccgta ggccagggag ccgcagggtc ggcggcgcag
     1561 gtcctggatc cggaggaagt cgatgatgtc ctcgacccga cgccggtgca cgacctcttc
     1621 tcgctgagcg aacccccagt agacgaacga agccaacact cccgacttca tgcgcacgtg
     1681 gcgaccgagc atgaggttgt cgagcacggt catgccgcgg aagagcgcga tgttctggaa
     1741 ggtccgcgcg atccccagct cggccaggtg actcggtgag tcacgagtga cgtcgctgcc
     1801 ttcgagcgcg atgcgcccgg agtccgggtg gtagaagccg ctgatcatgt tgagcagggt
     1861 ggtcttgccc gccccgttgg ggccgatgac ggacaggatc tcgccccggc gcacctcgag
     1921 gctcacccgg ctcaccgcca gcactccccc gaaggacttg gagacctccg cgatctcgag
     1981 ctgagcgctc atggccggac tctcacgaga gccaccgctt acggcgccgg tagttcttga
     2041 cgtcgcgata gctcttccgc tgtccgacac cggacagccc caggtagaac tccttgatgt
     2101 cctcgttctc gctgaccgcc ttgacgtcgc cgtccagcac cacccggccg ttctccatga
     2161 cgtacacgta ctgggcgaag cgcagggcca tgttggcgtt ctgctcggcg agcaggaccg
     2221 ccacccgctc ctcccggttg agccggccca cgatctcgaa gatctcctgc accagcatgg
     2281 gggcgagccc catcgagggc tcgtcgagca gcatcaggcg cgggtgcgcc atcagagcgc
     2341 ggccgatcgc gagcatctgc tgctcgcctc ccgagatgta gcccgcgtcg agcgagcgcc
     2401 ggtcccgcag ccgcgggaag tactcgtaga cccgctccag gtccgacttg agcgagtggc
     2461 cgttgcgccg ggtgtagccg ccggtcagca gattctcctc caccgtcaga tgctcgaaga
     2521 cgtggcggcc ctccatgacc tgcaccaccc ctcgccgcac gatctcggac gccgcgcgcc
     2581 ggtggatcgc ctccccaccg agctcgatgc cgcccttggt cacctcgccg cgctcggtcc
     2641 ggaggagccc cgagacggcc ttgagcgtgg tggacttccc ggcgccgt
//
LOCUS       DATMCV010000691         2440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_171111, whole genome shotgun sequence.
ACCESSION   DATMCV010000691 DATMCV010000000
VERSION     DATMCV010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171111"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VNU03_15760"
     CDS             <1..960
                     /locus_tag="VNU03_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWP75979.1"
                     /translation="PATGLLLSFETSRLMEVSVELRFVQVDVFTETVFGGNPLAVVFE
                     AGELDGGVMQRIAREMNCSETTFLVPATRADCAARVRIFTPAREIPFAGHPTIGTAWV
                     LATEGLLPAGTSQFNLEEGIGPVEVTLEGDSARPRFLWMRHGEAKFGPELADRAGFAR
                     ALGLEAADLLAGQPVRTGSTGNAFLYVPLRDREAVDRARLDVPALLRAQGEGPNLGVF
                     VFAPDPDPRAGRVYSRMFAPHTSGIPEDPATGSASGPLGAYLVERGLVAPADSVAIVS
                     EQGTRMGRPSFVHIRVGMRVGRVDEILVGGSVVPVIEGRLRIP"
     gene            975..1604
                     /locus_tag="VNU03_15765"
     CDS             975..1604
                     /locus_tag="VNU03_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HWP75980.1"
                     /translation="MYIPRHFEVTDTAWCHALMRAQSFATMITADDAGVPFATHLPML
                     VDDTRGPLGTLRGHVARANPHWRHLAAGRPTLVVFSGAHAYVSPAWYATHPAVPTWNY
                     IAVHATGTGSLVEDAERVRALLADLVHVYEGARPGAWSFEALAEDYVAGMQRGIVAFE
                     IPIDRLEGKAKLSQNRDAVDQGRTRDALAASDDPVARAVAGLMAGGPPR"
     gene            complement(1609..2379)
                     /locus_tag="VNU03_15770"
     CDS             complement(1609..2379)
                     /locus_tag="VNU03_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWP75981.1"
                     /translation="MADEVLVAVEAGIAVVTLNRPDQRNAMNTALLQGLRSAIDSLDE
                     RRDVRVVVVKGAGRAFCSGMDLKEMERRGGEADPEGNVVEVLRRVEASRHPTIAALHG
                     DAIAGGCELALHCDLRVAAEPVRLGMPLARIGLVIPFLLNQKLVEIIGPAHTRHLLFT
                     GQTVDARRAYEIGMVHQVVGAAELPAAVQALARRIADNAPLALAGIKASIQRTISARD
                     GIAHGDLDAAANRARTSADAQEGRRAMLEKRKPAFRGE"
BASE COUNT          303 a          839 c          931 g          367 t
ORIGIN      
        1 cccgcgacgg ggctgcttct atcttttgaa acgtcgcggc tcatggaggt gtcggtggag
       61 ttgaggtttg tgcaggtgga tgtgttcacg gagacggtgt tcggggggaa tccgctggcg
      121 gtggtgtttg aggctgggga gctggatggt ggggtgatgc agaggatcgc gcgggagatg
      181 aattgttcgg agaccacatt tctggtgccg gcgacgcggg cggattgtgc ggcgcgggtg
      241 aggatcttta cgccggcgcg ggagattccg tttgcggggc atccgacgat cgggacggcg
      301 tgggtgctgg cgacggaggg gctgctgccg gcggggacca gccagttcaa tctggaggag
      361 gggatcgggc cggtggaggt gacgcttgag ggggactcgg cccgccctcg ctttctgtgg
      421 atgcggcacg gggaggcgaa gttcgggccg gagctggcgg atcgggccgg gtttgcgcgt
      481 gcgctcggac tcgaggcggc ggacctgctc gcgggccagc cggtgcgcac cggctcgacc
      541 ggcaacgcct tcctctatgt cccgctgcgg gaccgggagg cggtcgaccg ggcccggctc
      601 gacgtgcccg cgctgctcag ggcgcagggc gagggaccga acctcggcgt cttcgtgttc
      661 gcgcccgatc cggatccgcg agccggccgc gtctactcgc gcatgttcgc gccgcacacc
      721 tcgggaatcc ccgaggaccc ggccaccggc tcggcgagcg gcccgctcgg cgcctacctg
      781 gtcgagcgcg ggctggtggc gccggccgac tccgtggcca tcgtgagcga gcagggcacg
      841 cgcatgggcc ggccgagctt cgtgcacatt cgcgtcggca tgcgggtcgg ccgggtcgac
      901 gagatcctgg tgggcggcag cgtggtcccg gtgatcgaag gccgcctgcg cattccctga
      961 tccgcgagag ggccatgtac ataccccgac acttcgaggt caccgacacc gcctggtgtc
     1021 acgcgctgat gcgggcgcag agctttgcga ccatgatcac cgccgacgac gcgggcgtgc
     1081 cgttcgccac ccacctgccg atgctggtgg acgacacgcg cgggcctctc ggtaccctgc
     1141 gcggccacgt ggcccgcgcc aacccgcact ggcgtcacct ggccgccggc cgccccacgc
     1201 tggtggtgtt ctcgggcgcg cacgcctacg tctcgcccgc gtggtacgcg acgcacccgg
     1261 cggtgcccac gtggaactac atcgcggtgc acgcgaccgg gaccggctcg ctcgtcgagg
     1321 acgcggagcg ggtgcgcgcg ctgctcgcgg acctggtcca cgtctacgag ggcgcgcgcc
     1381 cgggcgcctg gtccttcgag gcgctggccg aggactacgt ggccggcatg cagcggggga
     1441 tcgtggcgtt cgagatcccg atcgatcggc tcgagggcaa ggccaagctc agccagaacc
     1501 gcgacgcggt ggaccagggc cgcacccgcg acgcgctcgc cgcctccgac gacccggtgg
     1561 cccgcgcggt cgcgggcctg atggcgggcg gcccgccgcg ctgaggcgtt actccccccg
     1621 gaaggcgggc ttccgcttct cgagcatggc gcgccggccc tcctgcgcgt ccgcgctggt
     1681 gcgcgcgcgg ttggccgccg cgtcgaggtc tccgtgggcg atgccgtcgc gggccgagat
     1741 cgtccgctgg atgctcgcct tgatgccggc cagcgcgagc ggggcgttgt ccgcgatgcg
     1801 gcgcgcgagc gcctgcaccg cggcgggcag ctcggcggcc cccaccacct ggtggaccat
     1861 gccgatctcg taggcgcgcc gggcgtcgac ggtctgcccg gtgaacagca ggtggcgggt
     1921 gtgggcgggc ccgatgatct cgaccagctt ctgattgagc aggaacggga tcacgaggcc
     1981 gatgcgcgcg agcggcatgc cgagacgcac cggctccgcg gccacccgga gatcgcagtg
     2041 cagcgccagc tcgcagccgc ccgcgatggc gtcgccgtgg agcgcggcga tggtcggatg
     2101 ccgcgaggcc tccacccggc gcagcacctc caccacgttg ccttccggat ccgcctcacc
     2161 cccgcgccgc tccatctcct tcagatccat gccggagcag aacgcgcggc ccgccccctt
     2221 caccaccacc acgcgcacgt cgcgccgctc gtcgagggag tcgatggccg agcgcaggcc
     2281 ctgcagcagc gcggtgttca tcgcgttgcg ctgatccggc cggttcaggg tcacgacggc
     2341 gatgccggcc tcgacagcta ccagaacttc gtcggccatg gtggtctccg tgggctcgcg
     2401 tccgcggcta gccctcgccc ggggcgagga cgcggagcgc
//
LOCUS       DATMCV010000692         3430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_833550, whole genome shotgun sequence.
ACCESSION   DATMCV010000692 DATMCV010000000
VERSION     DATMCV010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833550"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /gene="purS"
                     /locus_tag="VNU03_15775"
     CDS             complement(<1..150)
                     /gene="purS"
                     /locus_tag="VNU03_15775"
                     /inference="COORDINATES: protein motif:HMM:NF014726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWP75982.1"
                     /translation="MKARVLVRLKPSILDAQGATVQRALAGLGFAEVQDVRVGKVVDV
                     ELTGLA"
     gene            complement(147..1460)
                     /gene="purB"
                     /locus_tag="VNU03_15780"
     CDS             complement(147..1460)
                     /gene="purB"
                     /locus_tag="VNU03_15780"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWP75983.1"
                     /translation="MISRYARPEMARIWTQDAKYEAWLQVELAVCEVYSRRGLIPVDA
                     LGRIKARARVSAERIDLIEATTRHDVIAFLTNLEEALGTDSRYVHMGLTSSDVVDTAL
                     ALQLQQASELLIAGLERFRGTLRALALRHRDTLCVGRTHGIHAEPMVFGLKAALWYSE
                     AGRNLERLRRARETVRVGKLSGAVGTFAHVDPDVEEEVCRLLGLEAALISNQIVQRDR
                     HAEFSTTLAIVAASLEKVATEIRALQRTEILEAEEPFAVGQKGSSAMPHKRNPISCEQ
                     VCGLARLVRTNALAALENVALWHERDISHSSVERVILPDSTCLVDYMLHQMTRIVEGL
                     QVYPERMRENMERSFGLLFSQRVLLKLTGKGLARQKAYEMVQRNAMTAWRDRTAFRDL
                     LASDPEVMAQLTRPELDECFDPTWHLRNVAAIYRRIGLPTEGGSE"
     gene            complement(1472..2731)
                     /locus_tag="VNU03_15785"
     CDS             complement(1472..2731)
                     /locus_tag="VNU03_15785"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWP75984.1"
                     /translation="MTDRKSALPNGIRLVTETMPHVRSVAVGIWVETGSRVEPEPLGG
                     ISHLIEHLVFKGTASRSAEDIARAIDSVGGHMDAFTAKEHTCFYVRVIDEHLPLAVEL
                     LTDILRHPRFAADDIEKEKAVVLQEIKMVEDTPDDLIHDLFAERVWAGHPLGRPILGQ
                     WDVVKGFDRDTVLGYFEREYVPSRIVVSVAGNAEHAQVADLFSAGFEGWDRAAEPHRL
                     TPPTIHPGIHTVQKPLEQVHLVMGLPGVPDAAPERHALYLLNDILGGSMSSRLFQEIR
                     ERQALAYSVHSGQQAYRDTGLFYVYAGTDPANFGKVMNALMKEIRSLKKDGVTAEELG
                     RSKDHLKGSLMLSLESTSSRMDRLARHEMRFGSFMSMDQMLAAIDAVRMEDVDALIHR
                     VLDEEQLSLMTLGAVNRRHFPRGLLRA"
     gene            complement(2731..>3430)
                     /locus_tag="VNU03_15790"
     CDS             complement(2731..>3430)
                     /locus_tag="VNU03_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWP75985.1"
                     /translation="EDHYGDMDFKVAGTDKGITALQMDIKIGGVSVDIMRQALAQARE
                     ARLHVLGKMAETIKGPRAELSPNAPRFLTIKIRPEKIREIIGPGGKVIRGIQEKTGAK
                     IDVEDDGKVTVFSPSAEAAQMAIGIIQDICREAELDRIYVGKVKSIKEFGAFVEIIPG
                     NEGLLHISQIAESRIRSVGDVLSEGDIVAVKVIEIDGNGKMRLSRKAAHRDQPGITEQ
                     EKLKIAPAAAGGSA"
BASE COUNT          491 a         1211 c         1174 g          554 t
ORIGIN      
        1 ggcgagaccg gtcagctcga cgtccaccac cttgccgacg cgcacgtcct gcacctccgc
       61 gaagcccagc ccggccaacg cccgctgcac ggtggcgccc tgggcgtcca ggatggacgg
      121 cttgaggcgg acgaggacgc gcgccttcac tcagagcctc cctccgtggg caggccgatc
      181 cgccggtaaa tggccgcgac gttgcgcagg tgccacgtcg gatcgaagca ctcgtccagc
      241 tcggggcggg tcagctgggc catcacctcg gggtcggagg ccagcaggtc gcggaaggcg
      301 gtgcgatcgc gccacgcggt catcgcgttg cgctgcacca tctcgtaggc cttctgccgg
      361 gcgagcccct tgccggtgag cttgaggagc acccgctggg agaagaggag accgaagctc
      421 cgctccatgt tctcccgcat ccgctccggg tagacctgca gcccctcgac gatgcgcgtc
      481 atctggtgga gcatgtagtc caccaggcac gtggagtccg gcaggatgac gcgctcgacc
      541 gaggagtggc tgatgtcccg ctcgtgccac agggccacgt tctccagcgc ggcgagcgcg
      601 ttggtgcgga ccagccgggc cagcccgcac acctgctcgc acgagatcgg gttgcgcttg
      661 tggggcatcg cgctcgagcc cttctgcccg accgcaaacg gttcttccgc ttccaatatc
      721 tcggtgcgct gcagcgcgcg gatctcggtg gcgaccttct cgagcgacgc ggccacgatg
      781 gccagggtgg tcgagaactc ggcgtgccgg tcccgctgca cgatctgatt ggagatgagc
      841 gcggcctcga ggccgaggag gcggcacacc tcctcctcca cgtccgggtc cacgtgggcg
      901 aaggtgccca ccgcgcccga gagcttgccc acccgcaccg tctcgcgcgc ccgccgcagc
      961 cgctcaaggt tgcgccccgc ctcgctgtac cagagggcgg ccttgaggcc gaacaccatc
     1021 ggctcggcgt ggatgccgtg ggtgcgcccc acacagagcg tgtcccggtg gcggagggcc
     1081 agcgcgcgca gcgtgccccg gaagcgctcg aggcccgcga tcaagagctc cgaggcctgc
     1141 tggagctgga gcgccagcgc ggtgtccacc acgtccgagg acgtgagccc catgtggacg
     1201 tagcgggagt ccgtccccag cgcctcctcg aggttggtca ggaacgcgat gacgtcgtgc
     1261 cgcgtggtgg cctcgatcag gtcgatgcgc tcggccgaga cccgggcgcg cgccttgatg
     1321 cgccccagcg cgtcgaccgg gatcagcccg cggcgcgagt agacctcgca caccgccagc
     1381 tccacctgga gccacgcctc gtacttcgcg tcctgggtcc agatccgcgc catctcgggg
     1441 cgcgcgtagc gggagatcac cggggagcct cctaggcgcg gagcaggccc cgggggaagt
     1501 gccgccggtt gaccgccccc agggtcatga gcgagagctg ctcctcgtcg agcacgcggt
     1561 ggatcagcgc gtccacgtcc tccatccgga ccgcgtcgat ggcggccagc atctggtcca
     1621 tggacatgaa cgagccgaac cgcatctcgt ggcgggccag ccggtccatg cgggacgagg
     1681 tcgactcgag cgagagcatg aggctgccct tcaggtggtc cttggaccgg ccgagctcct
     1741 cggcggtgac cccgtccttc ttcaggctgc ggatctcctt catcagcgcg ttcatcacct
     1801 tgccgaagtt ggccgggtcg gtgcccgcgt agacatagaa gagcccggtg tcccggtagg
     1861 cctgctggcc cgagtgcacc gagtaggcca gagcctgccg ctcgcgaatc tcctggaaga
     1921 gccgcgacga catgctcccg cccaggatgt cgttcagcag atagagcgcg tggcgctcgg
     1981 gcgcggcgtc cggcaccccg gggagcccca tgaccaggtg cacctgctcg agcggcttct
     2041 gcacggtgtg gatgcccggg tggatcgtag gcggcgtcag ccggtgcggc tcggcggccc
     2101 gatcccaccc ctcgaagccg gccgagaaga ggtcggccac ctgggcgtgc tcggcgttcc
     2161 cggccaccga caccacgatg cggctcggga cgtactcgcg ctcgaagtag cccagcacgg
     2221 tgtcccggtc gaagcccttc accacgtccc actgcccgag gatcggccgc ccgagcgggt
     2281 ggccggccca cacccgctcc gcgaacaggt cgtggatcag gtcgtccggg gtgtcctcca
     2341 ccatcttgat ctcctggagg acgaccgcct tctccttctc gatgtcgtcc gccgcgaagc
     2401 gcgggtgccg gaggatgtcg gtgagcagct ccaccgcgag cggcaggtgc tcgtcgatca
     2461 cccgcacgta gaagcaggtg tgttccttcg cggtgaacgc gtccatgtgc ccaccgaccg
     2521 agtcgatggc gcgggcgatg tcctccgcgg agcgcgacgc ggtgcccttg aagaccaggt
     2581 gctcgatcag gtgggagatg ccgcccaggg gctcgggctc gacccgcgag cccgtctcca
     2641 cccagatccc gaccgccacc gagcgcacat gcggcatcgt ctcggtgacg agacggatgc
     2701 cgttgggcag cgcgctcttg cgatcggtca ctaggcagag ccccccgccg cggcgggcgc
     2761 gatcttcagc ttctcctgct cggtgatccc gggctggtcc cggtgcgccg ccttgcgcga
     2821 gagccgcatc ttgccgttgc cgtcgatctc gatcaccttg accgccacga tgtcgccctc
     2881 ggagagcacg tcgcccaccg agcggatgcg gctctccgcg atctgggaga tgtggagcag
     2941 cccctcgttg ccggggatga tctccacgaa ggcgccgaac tccttgatcg acttcacctt
     3001 gcccacgtag atgcggtcga gctcggcctc gcggcagatg tcctggatga tgccgatcgc
     3061 catctgggcc gcctcggcgc tcggggagaa cacggtcacc ttgccgtcgt cctccacgtc
     3121 gatcttggcg ccggtcttct cctggatgcc gcggatgacc ttgccgccgg gtccgatgat
     3181 ctcccggatc ttctccgggc ggatcttgat ggtgaggaag cgcggcgcgt tcggcgagag
     3241 ctcggcgcgg ggccccttga tggtctcggc catcttgccc agcacgtgca gccgggcctc
     3301 acgggcctgg gcgagggcct ggcgcatgat gtccacggag acgccgccga tcttgatgtc
     3361 catctggagc gcggtgatcc ccttgtccgt gcccgcgacc ttgaagtcca tgtcgccgta
     3421 gtggtcctcg
//
LOCUS       DATMCV010000693         4189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_440116, whole genome shotgun sequence.
ACCESSION   DATMCV010000693 DATMCV010000000
VERSION     DATMCV010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440116"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VNU03_15795"
     CDS             complement(<1..294)
                     /locus_tag="VNU03_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWP75986.1"
                     /translation="MAADSISGVGTADGLSLPPRAVSRGRSGLGDRVYRTLILASVWL
                     VLLLAGGLVAALCWESGAAIRAFGFRFLVTSHWDPVAGEFGALPFVYGTLVSSL"
     gene            complement(298..1326)
                     /gene="pstS"
                     /locus_tag="VNU03_15800"
     CDS             complement(298..1326)
                     /gene="pstS"
                     /locus_tag="VNU03_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWP75987.1"
                     /translation="MLLLAAAMVGRPGLADAQMLMNGAGATFPYPIYSKWFEEYIKVD
                     PEVRFNYQSIGSGGGIRQITERTVDFGASDGPMTDEQLKKASAELLHIPTVMGAVVAT
                     YNLPGNPQLAFTGEVLADIFLGKITKWNDARIKALNPSATLPDQAIVVVHRSDGSGTT
                     YIWVDYLCKISPEWEKEVGRGTSVKWPVGLGGKGNEGVAGQVKNAPGSLGYVELAYAI
                     TNKLPAARIKNQAGKLVEPTIESTTAAAAGAVKSMPADFRVSLTNAPGPDAYPIASFT
                     WLLVYKDQPNESKGKALVKFLWWAIHDGQRHPAALLYAPLPAPVVKEIEAKIKQITFS
                     GKPLLAAQ"
     gene            complement(1451..2215)
                     /locus_tag="VNU03_15805"
     CDS             complement(1451..2215)
                     /locus_tag="VNU03_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWP75988.1"
                     /translation="MGVDPMAASRRSAADRPGAGMGRAGKVLVVEDEADVAEMIRYNL
                     GKEGYDVRLAANGTEALRQVKEARPDVILLDIMVPHLNGWEICRRLKQDRDTQAIPVI
                     MVTGRVEEGDKVLGFEMGADDYVTKPFSPRELVARVRAVSRRGRLGEDVTGQPVRAGD
                     LEIDRQRFEVKMKGRLVELTRKEFDLLSALVRSPGRVFGREELLDLVWGQDGFVEPRT
                     VDVHVARLRAKFIAARAPEPGIETVRGVGYRFRERS"
     gene            complement(2395..3456)
                     /locus_tag="VNU03_15810"
     CDS             complement(2395..3456)
                     /locus_tag="VNU03_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWP75989.1"
                     /translation="MAAAAAVGGWARPGRSAAAQPDEVKWRAFEVVTRAEVANPTGAT
                     RVWLPLPLQTDTDYFKSLGQSWTGNGAVTRVYRDERYGAGILYAEWPAGESAPAIEVT
                     TRFATRDRNVDVTRSGANPAPEDRATLAKYRESSKLVRTDGIVKKTSQQITKGIDTDV
                     EKARAIYEWIVDNTFRDPKVRGCGVGDIRAMLETGNLGGKCADLNALFVGLARAAGLP
                     ARDVYGIRVADSAEFKCLGKSGDITKAQHCRAEFYTAGLGWVPVDPADVRKVVLEERG
                     GIPLDDPTTRKARAKLFGQWEMNWLAYNYAHDVKLVNSKDEPVAFLMYPQAETGEGRK
                     DSLDPDAFKYKIASRELGT"
     gene            3656..>4189
                     /locus_tag="VNU03_15815"
     CDS             3656..>4189
                     /locus_tag="VNU03_15815"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWP75990.1"
                     /translation="MSPTSPDIEPLLTALQAQVEALHAREPEVRASRDPEDLRRMRVA
                     VRRLRATLRASRPLFDRSAVDGLRDELGWLGTALGRVRDLDMVGASVAAELGSLEGST
                     RRESHALLRRLDTDRTRAWDRLHVVLDGARYARLLRRLKTLLGRDPRHAAKVSLADAA
                     GAEWKKLRRAVKHLPGHP"
BASE COUNT          586 a         1524 c         1413 g          666 t
ORIGIN      
        1 cagcgaggac accagggtgc cgtagacgaa cggcagtgcg ccgaactcgc ccgcgaccgg
       61 atcccagtgc gaggtgacca ggaagcggaa gccgaaggcg cggatcgcgg cccccgactc
      121 ccagcagagc gccgcgacca ggccgccggc cagcagcagg accagccaca ccgaggccag
      181 gatcagcgtg cgatagacgc gatcgccgag tccgctgcgc ccccggctga ccgctcgcgg
      241 gggcagggac aggccgtccg ccgtccccac ccccgagatc gagtcggcgg ccaagcgcta
      301 ctgcgccgcc aggagcggct tgcccgagaa ggtgatctgc ttgatcttgg cctcgatctc
      361 ctttaccacc ggggccggca gcggcgcgta caggagcgcg gccgggtgcc gctgcccgtc
      421 gtggatcgcc caccagagaa acttcacgag ggccttgccc ttggactcgt tgggctggtc
      481 cttgtagacc aggagccagg tgaagctcgc gatcgggtag gcgtcgggtc ccggcgcgtt
      541 cgtgagcgag acccggaagt cggccggcat gctcttgacc gccccggccg ccgccgcggt
      601 cgtgctctcg atcgtcggct cgacgagctt gcccgcctgg ttcttgatgc gggccgcggg
      661 cagcttgttg gtgatcgcgt aggccagctc cacgtagccg agggagccgg gcgcgttctt
      721 cacctggccc gccacgccct cgttgccctt gccgcccagc ccgaccggcc acttcaccga
      781 ggtcccccgg ccgacctcct tctcccactc ggggctgatc ttgcagaggt agtcgaccca
      841 gatgtaggtg gtgccgctcc cgtccgaccg gtgcaccacc acgatggcct ggtccggcag
      901 ggtcgccgac gggttcagcg ccttgatccg cgcgtcgttc cacttcgtga tcttgccgag
      961 gaagatgtcg gccaggacct cacccgtgaa cgccagctgc gggttcccgg gcaggttgta
     1021 ggtggccacc accgcaccca tgacggtcgg gatgtgaagc agctcggcgg aggccttctt
     1081 gagctgctcg tcggtcatcg ggccgtcgga ggccccgaag tcgaccgtac gctcggtgat
     1141 ctgccggatg ccgccgccgc tcccgatcga ctgatagttg aagcgcactt ccgggtcgac
     1201 cttgatgtat tcctcgaacc acttcgagta gatcgggtac gggaaggtgg cccccgcccc
     1261 gttcatcagc atctgcgcgt cggccagacc cggccgtccg accatcgccg ccgccagcag
     1321 cagcatcggc aggattgacc acatcttcct catcggactc tcctcttgtc gtcttttccg
     1381 tgtggttggc ggaagtatgc tgcgagaggg tgacggatcg atgacgcagg tgttacatcc
     1441 gtgttaacag tcaggaccgc tcccggaagc ggtagccgac accgcgcacc gtctcgatgc
     1501 ccggctccgg cgcacgcgcc gcgatgaact tggcccggag ccgggccaca tggacgtcga
     1561 cggtgcgggg ctcgacgaag ccgtcctgcc cccacaccag gtccagcagc tcctcgcggc
     1621 cgaagacccg gccgggcgac cgaaccagcg ccgagagcag atcgaactcc ttgcgggtca
     1681 gctccaccag ccggcccttc atcttgacct cgaagcgctg gcggtcgatc tcgagatcgc
     1741 ccgcgcgtac cggctggccc gtcacgtcct cgccgaggcg tccgcgacgc gagaccgccc
     1801 gcacccgggc caccagctcg cggggggaga agggcttggt cacgtagtca tcggcgccca
     1861 tctcgaagcc gaggaccttg tcgccttcct ccacgcggcc ggtcaccatg atcaccggga
     1921 tcgcctgcgt atcccgatcc tgcttgagcc gccggcagat ctcccacccg ttgaggtgcg
     1981 ggaccatgat gtcgagcagg atcacgtcgg gccgcgcctc cttgacctgc cgcagggcct
     2041 cggtcccgtt ggcggcgagg cgcacgtcgt agccctcctt gccgaggttg tagcggatca
     2101 tctccgccac gtccgcctcg tcctccacca ccagcacctt cccggcccga cccatgccgg
     2161 cgcccggcct gtccgccgca ctccgccggc tcgccgccat cggatcgacc cccacgctcc
     2221 ctctcgtcat gttcatgggg gtatggtgcg cgcacggggt tacggaatga tgacgcgcgg
     2281 gtaaatcatg ggtaaacggc agtgtccgga catgcgcgac gcccccgcgg gggaggcggg
     2341 ggcgtcggtg gagccgggcg ccggacgggc gccccggtca ggagcggcgg ccggtcaggt
     2401 gcccagctcg cgcgaggcga tcttgtactt gaaggcgtcc gggtcgaggc tgtccttgcg
     2461 accctccccg gtctcggcct gagggtacat caggaacgcg accggctcgt ccttcgaatt
     2521 gaccagcttc acgtcgtgcg cgtagttgta ggcgagccag ttcatctccc actggccgaa
     2581 gagcttcgcg cgcgccttcc gggtggtggg atcgtcgagc gggatgccgc cccgctcctc
     2641 gagcacgacc ttgcgcacgt cggcgggatc gaccggcacc cagccgaggc ccgcggtgta
     2701 gaactcggcg cggcaatgct gcgccttcgt gatgtcgccg ctcttgccca ggcacttgaa
     2761 ctccgcggag tcggccaccc ggattccgta cacgtcgcgc gcggggagcc ccgccgcgcg
     2821 ggccaggccc acgaacagcg cgttcaggtc ggcgcacttg ccgccgaggt tgccggtctc
     2881 gagcatcgcg cggatgtccc cgaccccgca gccccgcacc ttcggatccc ggaacgtgtt
     2941 gtcgacgatc cactcgtaga tcgcgcgcgc cttctccacg tcggtgtcga tgcccttcgt
     3001 gatctgctgg gaggtcttct tcacgatgcc gtcggtgcgc acgagcttcg aggactcgcg
     3061 atacttcgcg agggtggccc gatcctcggg ggccggattc gcaccggacc gggtcacgtc
     3121 cacgttgcga tcgcgggtcg cgaaccgcgt cgtcacctcg atcgcgggcg cgctctcgcc
     3181 ggccggccac tccgcgtaga ggatccccgc gccgtaccgc tcgtcccggt acacgcgggt
     3241 gaccgccccg ttgccggtcc agctctgccc gaggctcttg aagtagtcgg tgtcggtctg
     3301 gagcgggagc ggcagccaca cccgcgtggc gccggtgggg ttcgccacct ccgcgcgcgt
     3361 gacgacctcg aaggcgcgcc acttgacctc gtccggctgg gctgccgccg agcgtcccgg
     3421 ccgggcccat ccgccgaccg cggcggccgc tgccatgccc atgcctgtct tcagaaagac
     3481 ccgtcgcttc atcgataccc cccggtcgat cgagtgcgcc gcgtggcggc aatggtacag
     3541 ggccgtgtag aaacgtccaa ttcgttgtac cgatgaggcc gactggcccc ataggcgacc
     3601 cctatggcga tgacggctgg cggcggcggc gcgcccatgt taccgatggg agcccatgtc
     3661 gcccacgagt cccgacatcg agccccttct cacggcgctc caggcccagg tcgaggccct
     3721 ccacgcccgc gagcccgagg tcagggcgag ccgcgatccg gaggatctcc ggcggatgcg
     3781 ggtcgcggtc cgccgtctgc gcgccaccct ccgggcgagc cgtcccctgt tcgaccggag
     3841 cgcggtcgat gggctgcggg acgagctcgg ctggctcggg acggcgctcg gtcgggtgcg
     3901 cgacctcgac atggtcggcg cctccgtcgc ggccgagctg ggctcgctcg agggctcgac
     3961 ccgccgggag agccacgcgc tcctccgtcg tctcgacacc gaccgcaccc gggcgtggga
     4021 ccggctgcac gtcgtgctgg acggcgcgcg atacgctcgg ctcctccggc gcctcaagac
     4081 gctcctcggg cgtgacccgc gccacgccgc caaggtctcg ctggcggatg ccgccggggc
     4141 cgagtggaag aagctgcggc gggcggtgaa gcacctcccg ggccatccg
//
LOCUS       DATMCV010000694         3262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_636275, whole genome shotgun sequence.
ACCESSION   DATMCV010000694 DATMCV010000000
VERSION     DATMCV010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636275"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VNU03_15820"
     CDS             complement(<1..609)
                     /locus_tag="VNU03_15820"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWP75991.1"
                     /translation="MKVLLVGLGRWGEKHLRVLGELGVELWVADVSAERRALAVKAGV
                     APARAVEDFRQALPHVDAVDVVTPADNHLALAGECLRAGRDCFIEKPLTLTVDEGRRL
                     AAIVAETGRVLQVGHIFRFHPVTTALRERIAAGVLGRVRYCTGRFAGFKRPRTDVGVT
                     QTDAIHYFDLFAHLLDRDATAVSATLRDHLGRGLDDCSFTTVE"
     gene            complement(606..1382)
                     /locus_tag="VNU03_15825"
     CDS             complement(606..1382)
                     /locus_tag="VNU03_15825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWP75992.1"
                     /translation="MTAAGSASPLASPLDLSIVLPVYNEEENLPLLWPEIRQVLEPTG
                     LRYEVIFVDDGSRDRSAEIIRAFREQDPRVRLVRLKVNAGETAATDAGIKAVRGRHVV
                     VMDADLQNDPHDIPGMLAHLDTWDAVTGWRVNRAAGDSWVRRLSSRIANRVRNRLSEE
                     TIQDSGCTFRAFRRECLRDLALYKGFHRFIPTLLRMRGFRVLEVPVNHRPRRFGVSKY
                     GIGNRALRAFVDLLVVRWMKDRLLRYEITEDVGGEGGDPR"
     gene            complement(1379..1972)
                     /locus_tag="VNU03_15830"
     CDS             complement(1379..1972)
                     /locus_tag="VNU03_15830"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012849237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HWP75993.1"
                     /translation="MKRLVVGISGASGAIYGIRFLELLRGIPDVESHLIVSDAGRRTI
                     VEETDYAVDAVEALAARRYRNRDIGAAVASGSFKTAGMVIVPCSIKSAAAVAHCAADT
                     LIARAADVTLKEGRPLVLVVRETPLHLGHLRVLTALAEMGAVILPPMPAFYNRPKQID
                     DLVTHTLARVLDRLGLPHTLAPEWQGTAPRVPPPAGA"
     gene            complement(1969..2253)
                     /locus_tag="VNU03_15835"
     CDS             complement(1969..2253)
                     /locus_tag="VNU03_15835"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HWP75994.1"
                     /translation="MSRERMFALADRIVADLTATESVMIKTADDKALGQLRTEVFRVL
                     EDESKLEESIDQEVRRSLSTYSRPAPEGSAEWELLYQKTRDEVYRRRFRL"
     gene            complement(2259..2507)
                     /locus_tag="VNU03_15840"
     CDS             complement(2259..2507)
                     /locus_tag="VNU03_15840"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HWP75995.1"
                     /translation="MAEAVVKRLVGRKLADVRDETRAREVVRRILMENFQAEEKIDAD
                     ARTLLMDNAKLIRDSASDYKRLFTMVKTKLAKERGFTL"
     gene            complement(2602..>3262)
                     /locus_tag="VNU03_15845"
     CDS             complement(2602..>3262)
                     /locus_tag="VNU03_15845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWP75996.1"
                     /translation="ELRTPLTALQGFSELLLRSALPPEKAHRCLVYIHTEACTLGRIV
                     GELLDLSRIEAGRALELHPETVDLDALLERNVELFATEHRQHRFEWTRTASGLALHAD
                     PDALDRMMKNLLSNAVKYSPRGGRVLVSASPAPDRPGMVEVAVEDDGVGIPAEQLPRI
                     FDKYVRVPHPDTAAARGLGLGLSLVKALAEAHGGRVEVDSLPGKGSRFRLLLPEVDSH
                     F"
BASE COUNT          472 a         1177 c         1146 g          467 t
ORIGIN      
        1 ctccacggtg gtgaacgagc agtcgtccag cccgcgcccc agatgatcgc gcagcgtcgc
       61 ggagaccgcg gtggcgtcgc ggtcgagcag gtgcgcgaag agatcgaagt agtggatggc
      121 gtcggtctgg gtcaccccca cgtcggtgcg cgggcgcttg aacccggcga agcgccccgt
      181 gcagtagcgc acgcggccga gcacgcccgc ggcgatccgc tcgcgcagcg cggtggtgac
      241 cgggtgaaag cggaagatgt gccccacctg gagcacgcgc ccggtctccg cgacgatcgc
      301 ggcgagtcgg cgcccctcgt ccaccgtgag ggtcagcggc ttctcgatga agcagtcgcg
      361 gcccgcgcgc aggcactccc cggccagggc cagatggttg tcggcggggg tcacgacgtc
      421 gaccgcgtcc acgtggggca gcgcctggcg gaagtcttcc accgcgcgcg cgggggcgac
      481 gcccgccttc accgcgagcg cccggcgctc ggccgagacg tcggccaccc acagctcgac
      541 ccccagctcc ccgagcacgc ggagatgctt ctcgccccag cggcccagcc cgaccagcag
      601 caccttcacc gcgggtcccc gccctcgccg cccacgtcct ccgtgatctc gtagcgcagc
      661 agccgatcct tcatccagcg caccaccagc aggtccacga acgcccgcag cgcccggtta
      721 ccgattccgt acttggacac gccgaagcga cgcggccgat ggttcaccgg cacctcgagg
      781 acgcggaacc cccgcatcct gagcagcgtg gggatgaacc ggtggaagcc cttgtagagg
      841 gccaggtcgc gcaggcactc gcgccggaag gcccggaagg tgcacccgct gtcctggatc
      901 gtctcctcgc tcaggcggtt gcgcacgcgg ttggcgatcc gcgaggagag gcgccgcacc
      961 caggagtcgc ccgcggcccg gttcacgcgc cagccggtca ccgcgtccca ggtgtcgagg
     1021 tgggcgagca tgcccgggat gtcgtgggga tcgttctgga ggtccgcgtc catgaccacc
     1081 acgtggcggc cgcgcaccgc cttgatgccc gcgtcggtcg ccgccgtctc ccccgcgttc
     1141 accttcaggc gcaccagccg cacccgcgga tcctgctcgc ggaaggcgcg gatgatctcc
     1201 gcgctccggt cgcggctgcc gtcgtccacg aagatcacct cgtagcggag cccggtcggc
     1261 tccaggacct ggcggatttc cggccagagc agcgggaggt tctcctcctc gttgtacacc
     1321 ggcagcacga tggagagatc gagcgggctc gcgaggggcg acgcggagcc ggcggcggtc
     1381 acgcgccggc gggcggcggc acgcgcggcg cggtgccctg ccattccggc gccagggtgt
     1441 gcggcagccc cagccggtcg agcacgcggg ccagtgtgtg ggtcaccagg tcgtcgatct
     1501 gcttcggccg gttgtagaac gcgggcatgg gcggcaggat caccgccccc atctcggcca
     1561 gggcggtcag cacccggagg tggcccaggt ggagcggggt ctcgcgcacg accaggacca
     1621 gcggccggcc ctccttgaga gtcacgtccg ccgcgcgcgc gatcagcgtg tcggcggcgc
     1681 agtgggcgac cgcggcggcg gacttgatgc tgcacggcac gatcaccatg ccggcggtct
     1741 tgaaggagcc cgacgccacc gcggcgccga tatcgcgatt gcggtagcgg cgcgcggcga
     1801 gcgcctcgac cgcgtccacc gcgtagtcgg tctcctcgac gatggtgcgc cgacccgcgt
     1861 cggagacgat caggtgggac tcgacgtcgg ggatgccgcg cagcagctcg aggaagcgga
     1921 tcccgtagat cgcgccggac gcgccactga tgcccacgac gagccgcttc acagccggaa
     1981 cctccgccgg tagacctcgt cacgggtctt ctggtagagc agctcccact ccgcgctgcc
     2041 ctcgggggcg gggcgcgagt acgtgctcag cgatcgccgg acctcctggt cgatcgactc
     2101 ctcgagcttg gactcgtcct ccagcacccg gaacacctcg gtgcgcagct ggccgagcgc
     2161 cttgtcgtcg gcggtcttga tcatcacgct ctcggtcgcg gtcaggtcgg ccacgatgcg
     2221 gtcggccagc gcgaacatgc gctcgcggct catccgggtc acagggtgaa cccccgctcc
     2281 ttggcgagct tggtcttcac catcgtgaag agccgcttgt agtcggaggc cgagtcccgg
     2341 atcagcttgg cgttgtccat caggagggtg cgggcgtcgg cgtcgatctt ctcctcggcc
     2401 tggaagttct ccatgaggat tcgccgcacc acttcccggg cccgcgtctc gtcccggacg
     2461 tcggcgagct ttcgccccac cagccgcttc accacggcct cggccatccg ccccgccacc
     2521 tcgtcccgtc gtggcatgcc cgaagagaca gggtacacgg cgtcgcgcga gggtgtcaag
     2581 cacaactatc tggaaaatag gtcaaaagtg gctatcgacc tcgggcagga gcaggcgaaa
     2641 ccgtgagccc ttgccgggca ggctgtccac ctcgacccgt ccgccgtggg cttcggccag
     2701 ggccttcact aggctcagcc cgagccccag cccccgcgcg gcggccgtgt cgggatgggg
     2761 cacgcgcacg tacttgtcga agatccgcgg aagctgctcg gcggggatcc ccacgccgtc
     2821 gtcctcgacc gccacctcca ccatcccggg ccgatccggc gccgggctcg cggagacgag
     2881 cacccgaccg ccccggggcg agtacttcac cgcgttcgag agcaggttct tcatcatgcg
     2941 gtcgagcgcg tccgggtcgg catggagcgc gaggccggag gcggtccggg tccactcgaa
     3001 ccggtgctgg cgatgctccg tcgcgaagag ctcgacgtta cgctccagca gcgcgtcgag
     3061 gtcgacggtc tcggggtgaa gctcgagcgc tcggcccgcc tcgatccgcg agagatccag
     3121 cagctcgccg acgatgcgcc cgagggtaca cgcctcggtg tggatgtaga cgaggcagcg
     3181 gtgcgccttc tcgggcggga gcgcggagcg gagcagcagc tcgctgaacc cctggagcgc
     3241 ggtcagcggc gttcgcagct cg
//
LOCUS       DATMCV010000695         3037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_905493, whole genome shotgun sequence.
ACCESSION   DATMCV010000695 DATMCV010000000
VERSION     DATMCV010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905493"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VNU03_15850"
     CDS             <1..449
                     /locus_tag="VNU03_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HWP75997.1"
                     /translation="QDALDAWLGAAQARVILPITDPYVVHHGALGSFATVYLPAGVDA
                     EQMRERVAALPGMEVVLGREAACRRFELPPDRVGDLVAVSERHVVIGTSEARHDLSGL
                     DAPLRSHGGISEQRVPLLLSRPTPGVPADQRLRNFDVFDLALNHVA"
     gene            complement(582..1817)
                     /gene="phnA"
                     /locus_tag="VNU03_15855"
     CDS             complement(582..1817)
                     /gene="phnA"
                     /locus_tag="VNU03_15855"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HWP75998.1"
                     /translation="MATRRASTLTVNGRSYAWPDRPLVVMCIDGCEPDYVRQAIGAGA
                     MPYLASALPGGTERLADCVVPSFTNPNNLSIVTGVPPAAHGICGNYFFDREAGAEVMM
                     NDPRYLRCGTILAAFAREGARVAVVTAKDKLRRLLGHEMSGVCFSAEKADETTVAEHG
                     IADGLGLVGMPLPSVYSAELSEFVFAAGVKLLESARPDIMYLSTTDYVQHKHAPGTPE
                     ANAFYGMMDGYLAKLAGLGATLAITADHGMNAKTDASGRPRVIYLQDAFDAWLGAAQA
                     RVILPITDPYVVHHGALGSFATVYLPAGTDAERLRERVAALPGMEVVLGREAACRRFE
                     LPPDRVGDLVAVSERHVVIGTSEARHDLSGLDAPLRSHGGISEQRVPLLLSRPTPDVP
                     AARRLRNFDVFDLALNHVG"
     gene            complement(1819..>3037)
                     /locus_tag="VNU03_15860"
     CDS             complement(1819..>3037)
                     /locus_tag="VNU03_15860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP75999.1"
                     /translation="TCPLAALGGKETGGMNVYVRQVARELGRMGLHVDVFTRSQNPTI
                     PRVVELGPGARVVHLPAGPEAPMPREAIHPHLGQFAAGVEDFARQSGITYDLIHSHYW
                     LSGVAGLRLRDKWGAPLVQMFHTLGRLKNTVAQSEADLEPALRIEEETRIVAEADRVV
                     AANVVERAHLVWHYGARADRIAVIPCGVDTDLFQPMDPAKAKDLLELPPDPLLLYVGR
                     MQPIKGLDTLLEAMTAVPEPAYLLIVGGEHDERENAHGAALRRRLSALGLERRVRFLR
                     SQPQRRLRLFYAAAAATVVPSHYESFGMVALEAMACGSPVVASRVGGLTTTVQDGVTG
                     RLVPEGDPAALAAAITALLDGAEGRRLGQQAIRWAAEHRWPCVAEAVCRLYSDLRPGA
                     VQHLPQAQCRGWS"
BASE COUNT          423 a         1112 c         1101 g          401 t
ORIGIN      
        1 tccaggacgc gctcgacgcg tggctcggcg cggcccaggc gcgagtgatc ctgccgatca
       61 ccgatcccta cgtcgtccac cacggggccc tcggctcctt cgcgaccgtc tacctgccgg
      121 ccggcgtcga cgcggagcag atgcgcgagc gcgtggcggc gctgcccggg atggaggtgg
      181 tgctggggcg cgaggcggct tgccggcgct tcgagctgcc gcccgaccgg gtgggcgatc
      241 tggtggccgt ctccgagcgg cacgtggtga tcggcaccag cgaggcgcgc cacgacctct
      301 ccgggctcga cgctccgctg cgctcgcacg gcggcatcag cgagcagcgt gtgccgctcc
      361 tgctaagccg ccccaccccc ggcgtccccg ccgaccagcg cctgcggaac ttcgacgtat
      421 tcgacctcgc gctgaatcat gtggcgtgag ataagagccg ccgccgcaaa cggccgtccg
      481 ccactggggg agtgcggggg ccatttctgg gcccccgcag atgaagagcc tgaagaacta
      541 aacggtcgcc cgctggccgg tcgcccgctg accccccgcc ctcaccccac atgattcagc
      601 gcgagatcga acacgtcgaa attccggagc cggcgcgcgg caggcacgtc aggggtgggg
      661 cggctcagca ggagcggcac ccgctgctcg ctgatgccgc cgtgcgagcg cagcggagcg
      721 tcgagcccgg agaggtcgtg gcgcgcctcg ctggtgccga tcaccacgtg ccgctcggag
      781 acggcgacca gatcgcccac ccggtcgggc ggcagctcga agcgccggca ggccgcctcg
      841 cgccccagga ccacctccat cccgggcagc gccgccacgc gctcccgcag ccgctccgca
      901 tccgtgccgg ccggcaggta gacggtcgcg aaggagccga gggccccgtg gtggacgacg
      961 tagggatcgg tgatcggcag gatcacccgc gcctgggccg cgccgagcca cgcgtcgaac
     1021 gcgtcctgga ggtagatcac gcgcgggcgg ccgctcgcgt cggtcttggc gttcatcccg
     1081 tgatcggcgg tgatcgcgag cgtcgcgccg agcccggcca gcttggcgag gtagccgtcc
     1141 atcatcccgt agaacgcgtt ggcctccggg gtgcccggcg cgtgcttgtg ctgcacgtag
     1201 tcggtggtgg agaggtacat gatgtccggg cgcgccgact cgagcagctt cacccccgcc
     1261 gcgaacacga actccgacag ctccgcgctg tacaccgacg gcagcggcat cccgaccagg
     1321 ccgaggccgt cggcgatgcc gtgctcggcc accgtcgtct cgtcggcctt ctcggccgag
     1381 aagcagacgc cggacatctc gtgtccgagc agccggcgca gcttgtcctt ggcggtgacc
     1441 accgcgacgc gggcgccctc gcgggcgaag gcggccagga tggtgccgca ccgcaggtag
     1501 cgggggtcgt tcatcatgac ctccgccccc gcctcgcgat cgaagaagta gttgccgcag
     1561 atcccgtgcg cagcgggtgg caccccggtc acgatcgaga ggttgttggg gttggtgaag
     1621 ctcggcacca cgcagtcggc caggcgctcg gtgcccccgg gcagcgccga ggccaggtac
     1681 ggcatcgccc ccgccccgat ggcctgccgc acgtagtcgg gctcgcagcc gtcgatgcac
     1741 atcaccacca gcgggcggtc cggccacgca tacgagcggc cgttcacggt cagggtggag
     1801 gctcgtcgag tggccatgtc agctccagcc ccggcactgg gcctggggaa gatgctgcac
     1861 cgctcccggg cgcagatcgg agtagaggcg gcacaccgct tcggccacgc agggccagcg
     1921 gtgctcggcc gcccagcgga tggcctgctg gccgagccgg cggccctcgg cgccgtcgag
     1981 cagcgcagtg atcgcggcgg ccagcgcggc cgggtcgccc tcgggcacga gccggccggt
     2041 caccccgtcc tgcaccgtgg tggtgagccc gcccacgcgt gaggccacca ccgggctgcc
     2101 gcacgccatc gcctcgagcg cgaccatccc gaacgactcg tagtgcgacg ggaccaccgt
     2161 cgcggccgcc gcggcataga acagccggag gcggcgctgg ggctgggacc ggaggaagcg
     2221 cacccgccgc tccagcccga gcgcgctcag gcgccggcgc agcgcggccc catgggcgtt
     2281 ctcgcgctcg tcgtgctccc cgcccacgat cagcaggtac gcgggctccg gcaccgcggt
     2341 catcgcctcg agcagcgtgt cgagcccctt gatcggctgc atccggccca cgtagaggag
     2401 gagcgggtcc ggcggcagct cgagcaggtc cttggccttc gcggggtcca tcggctggaa
     2461 caggtcggtg tcgacgccgc acgggatcac cgcgatgcga tcggcgcggg cgccgtagtg
     2521 ccagaccagg tgcgcacgct cgacgacgtt ggccgccacc acccgatcgg cctcggccac
     2581 gatgcgggtc tcctcctcga tgcgcagcgc gggctcgagg tcggcctcgc tctgagccac
     2641 cgtgttcttg agtcggccga gggtgtggaa catctgcacg agcggggcgc cccacttgtc
     2701 ccggagccgc aggccggcca ccccggaaag ccagtagtgc gagtggatca ggtcgtaggt
     2761 gataccgctc tggcgggcga agtcctcgac gccggccgcg aactggccca gatggggatg
     2821 gatcgcctcg cgcggcatcg gggcctcggg acccgcgggc aggtgcacca cgcgggcgcc
     2881 gggaccgagc tcgaccaccc gggggatcgt cgggttctgc gaccgggtga agacgtcgac
     2941 gtgcagcccc atgcgcccga gctcgcgcgc gacctgccgc acgtagacgt tcatcccgcc
     3001 ggtctccttg ccgcccagcg cggcgagggg acacgtg
//
LOCUS       DATMCV010000696         8224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_905509, whole genome shotgun sequence.
ACCESSION   DATMCV010000696 DATMCV010000000
VERSION     DATMCV010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905509"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VNU03_15865"
     CDS             complement(<1..786)
                     /locus_tag="VNU03_15865"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HWP76000.1"
                     /translation="MRFGFGMRRAALMMAVLGLALGGCGTLGGGLKSESLLAATSAPT
                     DTTDPANPVAAALAPEQQSTGAVVGPHAGEQVLAASVAFSPASFERIAAGRQPSYMVA
                     SIGPLMVSQVTGPLLAQARAADPVVPSEGILEEYDPWEPFNEKMFSFNYNMDKYVLKP
                     VAKGYNFVMPDRLQQMIDSGFDNLLVVQRVVNNLLQWRPKGAGAEVGRFLINSTLGIG
                     GLFDIAKQEFGLQKTRVDFGQTLGIWGVNPGPFLVLPFLPPLTV"
     gene            1109..1516
                     /locus_tag="VNU03_15870"
     CDS             1109..1516
                     /locus_tag="VNU03_15870"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWP76001.1"
                     /translation="MIELTLDMAERAARAAREKARALGTPITVTVVDEAGRLVLCARG
                     DGTGFFTPETSRAKALAAANFRMSTADLAERVQKGGAFWAQVGSAIPGQILASHGAVP
                     IVVGGRVIGAIGCGGGTGEQDQECAAAGAAVLA"
     gene            complement(1520..3619)
                     /locus_tag="VNU03_15875"
     CDS             complement(1520..3619)
                     /locus_tag="VNU03_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWP76002.1"
                     /translation="MSDLSAVFRPRAIAVVGASDDPVKIGGRPLAFLLRHGYPGHVYP
                     VNPSRATVQGLPAFPSVAAIPGEVDLAIVVVPAERVLESLEAAAAKGIRAAIVFSSGF
                     AEVGEAGRSAQAQLRALAERTGLRIIGPNCQGFAHLPSRLVATFASPFLDTGLATGPI
                     AMVSQSGAMAGMIYEMARAAGLGLNYWVSTGNEADVQAAEILAEVVEDPETRVACTYL
                     EDVKDAGRLRDALARAHRRGVPVFVLKSGRSAVGRRAASSHTGALAGEDAVYDAVFAD
                     WGAIRCADPAELLALPQAFLRYRDAGARVAILSNSGGLGVLSVDLCVDLGLVPAEFTP
                     ETTAILRAALPDFAAAANPVDLTAQMLTDPGMLARVLPALEADPGVDAIVFQIALLGA
                     ATDLGRLVQDVASVARRTPKVVAVSCPQRPVVDAFRAGGVLAFEDGSVALRSLACLAR
                     ATARRSRWLARVTEPSPPPTAPPALPPGRRSGQFLNEWESQRLLAPYGLPLVDTVFVT
                     AAAAAPDAADQVGYPVVVKICSAALPHKSDVGGVVLGLPDRASVAEACRRIDAEVRAK
                     APDVAVEGFVIQRQARGALELALGVKTDPVFGPVVMVGSGGVLIEVLRDFRLLLPPID
                     AAAAQEALRALRIGALWDGVRGSAPLDLPAAVALLERLGEAARALGPVVSEIDLNPVL
                     VGRRGEGVTVLDALVKL"
     gene            complement(3616..4164)
                     /locus_tag="VNU03_15880"
     CDS             complement(3616..4164)
                     /locus_tag="VNU03_15880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017686919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HWP76003.1"
                     /translation="MRDRTALLVIDMQIGLVEGAYRADAVLDRIAELIGRARRAGVPV
                     IHIQHHHATYAPLKAGAPTWHLHPRVAPAEGERVIGKTASDAFDETPLLGELRRLDVT
                     HLVVAGMQTELCVDTTCRRAVTLGLDVTLASDAHTTVDATLPAESIVAHHNAVLPQLA
                     HPHHHILVRPSRDVTFGYTPAP"
     gene            4206..6542
                     /locus_tag="VNU03_15885"
     CDS             4206..6542
                     /locus_tag="VNU03_15885"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP76004.1"
                     /translation="MARWDLRFSLVVAASLAVLVGLVADLALDAGRPYPGFFAAPDFR
                     LFPAEPDARAAGLRLGDRLIAVDGASPLTLAKRVRAAAGPIHYEVERAGRRLTVELAP
                     RPLDWSRLIDRFGIYVLVSAIMLGVGALVYVQNPAAAPNQRFLLYMCLWAVSNVAVPE
                     AVLGARTSAATVVGLVAPLLSIHGWVFFLTYPANPARERWLERHRVIPRLYRLAVALG
                     VAGALAYVLIVWRAPALLVDGWIYPASAAVLSLLSALSFPIKVGALLDTKRRAASPLI
                     DQQTTVLLLGIAMGLGGWLCLMLWPLQQLYASPVDPQVGSALMLLYPAAIGYATVRYR
                     LFDATVVIRRSVIYTGLAGLITGAYALLLAGANALLAQADLTRSPWFSAGFMFLVALL
                     FNPLRERVRRAVDRTFFRERHDWVQAMRTLARSMSSLLDLEEITRRLTTTIESAMHVR
                     TVHLLVDGLPHDVLAALEIAPGALSRYQVAADPRFAAAGGAALGAYQALGAEVVVPLR
                     FQAQLRGLLVLGAKQSDAAYTGEDLEMLEALADQAAVAVANAEAHRQVLEYAEQLERS
                     LLIRSSLAKFVPRRVRQLIEESPEAPSLEKRETDVTVLFADISGYTRLSARLQPDDLA
                     ALVERYFGAFLDEIVKHGGDVNETAGDGLMVIFHEGEHARAAVDAARAIQRRAAEIGA
                     ELAARFDPVAMHVGINTGPALLGATKIEGSAGTRWTYTASGMTTNIAARLSAQAGDGE
                     IVLSEPTRRRLDADLAVEDLGVRELKNVDTPLRLFRLR"
     gene            6694..>8224
                     /locus_tag="VNU03_15890"
     CDS             6694..>8224
                     /locus_tag="VNU03_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76005.1"
                     /translation="MELTRRDLLKGAAALGAAAAVPLTAGGPALAQTTQKKELITAQS
                     GDVSKFDPHYSTSSNDVRVSFNLFDNLTSRHPDGKLYPGLATEWKLEGPTTWRFKLRQ
                     GVKFHNGDPLTSADVKYSIERTYDPAAKTMVATVFGTIEKVDAPDASTIVITTKKPDP
                     LLAARLAFYGGQVVPKKYLESVGNDAFNAKPIGSGPVRFTSWVKDDRAILEANPDYWG
                     GKIDMDRWIMRPIPETAPRIAALLKGEVDAITQLPPDQEDRVAANPTTRVAGALYAGL
                     YVLAVNSKRPPLDNPLVKQALSLAIDREAIVKELWRGRGVVPNGPIAKGDNHFDASLP
                     PLAYNPAEAKARLQKAGYKGEEVFIETTVAYVAQDKAMAEAIAAMWKDVGVNVKVEVI
                     EYSVRAQKNREKSFKGVFWSDPTSTLSDPDGMMWRLLGPGGPQDYWREAKFDELGNAA
                     RFSVDEKFRGQSYKEMTKIFLEHLPWLPVIQPYEDYGIQKYIDFTPNPNQQFEIRRFN
                     FK"
BASE COUNT         1206 a         2939 c         2974 g         1105 t
ORIGIN      
        1 cacggtgagg ggaggcagga acggcagcac caagaagggc ccgggattca ccccccagat
       61 accgagggtc tgcccgaagt cgacgcgggt cttctggagc ccgaactcct gcttggcgat
      121 gtcgaagagc ccgccgatgc ccagcgtgct gttgatcagg aagcgcccga cctcggcgcc
      181 cgcgcccttg ggccgccatt gcagcaggtt gttcaccacc cgctggacca ccaggaggtt
      241 gtcgaacccg ctgtcgatca tctgctgcag ccggtcgggc atgacgaagt tgtagccctt
      301 ggcgaccggc ttcagcacgt acttgtccat gttgtagttg aacgagaaca tcttctcgtt
      361 gaacggctcc cacgggtcgt actcctcgag gatgccctcc gagggcacca ccggatcggc
      421 cgcgcgcgcc tgggccagca ggggcccggt gacctggctc accatcagcg ggccgatcga
      481 cgcgaccatg taggagggct gccggcccgc ggcgatccgc tcgaacgagg cgggggagaa
      541 ggccacgctg gcggccagca cctgctcacc cgcgtgcggc ccgacgacgg cgccggtcga
      601 ctgctgctcg ggcgcgagcg cggccgcgac cgggttcgcg ggatcggtcg tatccgtcgg
      661 cgcgctggtg gccgcgagca gcgactcgct cttgagccca cccccgagcg tgccgcagcc
      721 cccgagagcc aggcccagaa ccgccatcat cagcgctgcc cgtcgcatgc cgaatccgaa
      781 gcgcaccgtc tctctccctc cagaatgccg attggggtgg ctcaccagcc cgccgctcga
      841 ggccgacgcg gccagggcac caaagggagc aaggcaggtg ccagagcgca tcaggcccga
      901 gcatagggta tggagcgaca ttgtttcaag cactttccct gagacacgcc gtcgcctcgc
      961 gcccctacct atagtcgtac gccgcgcacg atttgccagc gcggtcaccg aatccggtcc
     1021 gcgggccgtt gacaggccgg gatcggcgcg attcaatgcg ggcgcggtcg cgggatcccg
     1081 gtggtgcgat gcgagaaagg tggcggagat gatcgagctg acgctggaca tggccgagcg
     1141 cgcggcgcgt gcggcgcggg agaaggcgcg ggccctcggc acgccgatca cggtgacggt
     1201 ggtggacgag gcgggccgcc tggtgctctg cgcccgcggc gacgggaccg gcttcttcac
     1261 gcccgagacg tcgagggcca aggcgctggc cgcggcgaac ttccgcatgt ccaccgccga
     1321 cctcgccgag cgagtgcaga agggcggcgc cttctgggcg caggtgggca gcgcgatccc
     1381 cggccagatc ctggcgagcc acggcgcggt gccgatcgtg gtgggcggcc gcgtgatcgg
     1441 cgcgatcggc tgcgggggcg gcactggcga gcaggaccag gagtgcgcgg cggcgggcgc
     1501 cgcggttctc gcctgagggc tagagcttca cgagcgcgtc gaggacggtc acgccctcgc
     1561 cgcgccggcc caccaggacc gggttcaggt cgatctcgct gaccaccggc ccgagcgcgc
     1621 gcgcggcctc gccgaggcgc tcgagcaggg cgaccgccgc gggcaggtcg agcggcgcgc
     1681 tgccgcgcac gccgtcccac agcgcgccga tgcgcagggc gcggagcgcc tcctgggccg
     1741 cggcggcgtc gatcgggggc agcagcagcc ggaagtcgcg cagcacctcg atgagcacgc
     1801 cccccgatcc caccatgacc accgggccga acaccggatc ggtcttcacc ccgagcgcca
     1861 gctcgagcgc accgcgggcc tggcgctgga tcacgaagcc ctcgaccgcg acgtcgggcg
     1921 ctttcgcccg cacctccgcg tcgatgcgcc ggcacgcctc cgcgaccgag gcgcggtcgg
     1981 gcagcccgag cacgacgccg cccacgtcgg acttgtgggg cagggcggcc gagcagatct
     2041 tcaccaccac cgggtagccg acctggtccg ctgcgtcggg cgcggcggcg gcggccgtga
     2101 cgaacaccgt gtcgaccagg ggcaggccgt agggcgcgag caggcgctgg ctctcccact
     2161 cgttgaggaa ctgcccggag cgacggcccg gcggcagcgc gggcggcgcg gtcggcggag
     2221 gcgagggttc ggtgacccgc gcgagccagc gcgagcgccg cgcggtcgcc cgcgccaggc
     2281 aggcgagcga gcggagcgcc acgctgccgt cctcgaaggc gagcacgccg ccggcccgga
     2341 aggcgtcgac caccggccgc tgtgggcagc tcaccgccac gaccttcgga gtgcgccggg
     2401 cgaccgaggc gacgtcctgg acgagccggc ccagatcggt cgcggcgccg agcagggcga
     2461 tctggaagac gatcgcgtcg acgcccgggt cggcctcgag cgcggggagc acgcgggcca
     2521 gcatgccggg atcggtcagc atctgcgcgg tgaggtcgac cggattggcg gccgccgcga
     2581 agtcgggcag ggccgccctt aggattgcgg tcgtctcggg cgtgaactcg gcgggcacca
     2641 ggcccagatc cacgcagaga tcgaccgaga gcacgccgag cccgcccgag ttcgacagga
     2701 tcgcgacgcg cgccccggcg tcccggtagc gcaggaaggc ctggggcagg gccagcagct
     2761 cggcgggatc ggcgcagcgg atggcgcccc agtccgcgaa caccgcgtcg tagactgcgt
     2821 cctcgccggc gagcgcgccg gtgtgggacg acgcggcgcg ccggcccacc gcgctccggc
     2881 ccgacttcag cacgaagacc ggcaccccgc ggcgatgggc gcgtgcgagc gcatcgcgca
     2941 gccggcccgc gtccttgacg tcctcgaggt aggtgcaggc cacgcgcgtc tcgggatcct
     3001 cgaccacctc ggccaggatc tcggccgcct gcacgtcggc ctcgttgccg gtgctcaccc
     3061 agtagttgag tccgaggccg gccgcgcgcg ccatctcgta gatcatcccg gccatcgccc
     3121 cgctctggct caccatcgcg atcgggccgg tcgcgagccc ggtgtcgagg aagggcgacg
     3181 cgaaggtcgc gaccaggcgc gagggcaggt gggcgaagcc ctggcagttg ggcccgatga
     3241 tgcggagtcc ggtgcgctcg gccagggcgc gcagctgggc ctgagccgag cgcccggcct
     3301 ctccgacctc ggcgaagccg ctgctgaaca cgatggcggc ccggatgccc ttggccgccg
     3361 cggcctcgag cgactcgagc acccgctcgg ccggcaccac cacgatcgcg agatcgacct
     3421 caccggggat cgcggccacc gagggaaagg cggggagccc ctgcacggtg gcgcgcgagg
     3481 gattcaccgg gtacacgtgg cccggatagc cgtgacggag gaggaaggcg agcgggcgcc
     3541 cgccgatctt gaccggatcg tcggaggcgc ccaccaccgc aatggcgcga ggccggaaca
     3601 ccgcgctcag atcgctcacg gagcgggagt atagccgaag gtcacgtcgc gactcgggcg
     3661 cacgaggatg tggtggtgcg ggtgggccag ctgcggcagc accgcgttgt gatgggcgac
     3721 gatcgactcg gccggcagcg ttgcgtcgac cgtggtgtgg gcgtcgctcg ccagcgtcac
     3781 gtccagtccg agggtgacgg cgcggcgaca ggttgtgtcg acgcagagct cggtctgcat
     3841 cccggccacg accaggtggg tcacgtcgag gcgtcgcagc tcgcccagca ggggggtctc
     3901 gtcgaaggca tccgacgcgg tcttgccgat gacccgctcg ccctccgccg gggccacccg
     3961 cggatgcaga tgccacgtcg gcgcgccggc cttcagcggg gcgtaggtgg cgtggtggtg
     4021 ctggatgtga atgacgggca cgccggcccg acgggcgcgg ccgatgagct cggcgatccg
     4081 gtcgagcacc gcgtccgccc ggtaggcgcc ctcgacgagg ccgatctgca tgtcgatcac
     4141 cagcaaggcg gtccggtcgc gcatgggtag atcgtcatca tagcgtgtat gatcggcgcg
     4201 agagcatggc gcgctgggac ctccgcttct cgctcgtcgt cgcggcgagc ctcgcggtgc
     4261 tggtgggcct ggtggccgac ctggccctcg acgcgggccg gccgtatccc ggcttcttcg
     4321 ccgcccccga tttccgcctc ttccccgccg agcccgacgc gcgcgcggcc ggcctccgcc
     4381 tgggcgatcg cctgatcgcg gtcgacggcg cctccccgct cacgctggcc aagcgggtgc
     4441 gggccgcggc cgggcccatc cactatgaag tcgagcgggc gggccggcgg ctcaccgtcg
     4501 agctggcgcc gcgcccgctc gactggagcc ggctgatcga ccgcttcggg atctacgtgc
     4561 tcgtctccgc gatcatgcta ggggtcggcg cgctggtcta cgtgcagaac ccggccgccg
     4621 cgccgaatca gcgcttcctc ctctatatgt gtctctgggc ggtctcgaac gtcgcggtgc
     4681 cggaggcggt gctgggcgcg cgtacctcgg cggcgacggt ggtgggcctg gtcgcgccgc
     4741 tgctctcgat ccacggctgg gtcttcttcc tcacgtaccc ggccaatccg gcgcgcgagc
     4801 ggtggctcga gcgtcaccgg gtgatcccgc ggctctatcg cctggcggtg gccctcgggg
     4861 tggccggcgc gctcgcctac gtgctgatcg tgtggcgcgc tcccgcgctg ctggtggacg
     4921 gctggatcta cccggcctcc gccgcggtgc tctcgctgct gagcgcgctc tcgttcccga
     4981 tcaaggtggg cgcgctgctc gacaccaagc gccgcgcggc ctcgcccctc atcgaccagc
     5041 agaccaccgt gctcctgctc ggcatcgcga tggggctcgg cgggtggctc tgcctgatgc
     5101 tctggccgct ccagcagctc tacgcgagcc cggtggaccc gcaggtgggc tcggccctga
     5161 tgctgctcta cccggccgcc atcggctacg ccaccgtgcg ctaccggctc ttcgacgcca
     5221 ccgtggtcat ccggcgctcg gtcatctaca ccgggctcgc cggcctgatc accggcgcct
     5281 acgcgctgct gctggcgggc gccaacgcgc tgctggccca ggccgacctc acccggtcgc
     5341 cgtggttctc ggccggcttc atgttcctgg tggcgctgct cttcaacccg ctgcgcgagc
     5401 gggtgcggcg cgcggtggac cgcacgttct tccgcgagcg ccacgactgg gtgcaggcca
     5461 tgcgcacgct ggcccgctcg atgtcgagcc tgctcgacct cgaggagatc acccgtcgcc
     5521 tcaccaccac catcgagtcc gccatgcacg tgcgcaccgt ccacctgctg gtggacggcc
     5581 tcccgcacga cgtgctcgcc gcgctggaga tcgcgcccgg cgcgctctcc cgctaccagg
     5641 tggccgccga tccgcgcttc gcggcggcgg gcggcgccgc gctcggggcc taccaggccc
     5701 tcggcgcgga ggtggtggtg ccgctgcgct tccaggccca gctgcgcggg ctgctcgtgc
     5761 tgggggccaa gcagtcggac gcggcctaca ccggcgagga tctggagatg ctggaggcgc
     5821 tcgccgacca ggccgcggtc gcggtggcca acgcggaggc ccaccggcag gtgctcgagt
     5881 acgcggagca gctcgagcgc agcctgctca tccgcagtag cctcgccaag ttcgtgccgc
     5941 gccgggtgcg ccagctcatc gaggagtcgc cggaggcgcc gtcgctcgag aagcgcgaga
     6001 ccgacgtcac ggtgctcttc gcggacatca gcgggtacac gcgactctcc gcgcgcctcc
     6061 agcccgacga cctggccgcc ctcgtcgagc gctacttcgg cgcgttcctc gacgagatcg
     6121 tcaagcacgg gggcgacgtc aacgagaccg cgggcgacgg gctgatggtg atcttccacg
     6181 agggcgagca cgcgcgcgcc gcggtggacg ccgcgcgcgc gatccagcgc cgcgcggccg
     6241 agatcggggc cgagctggcc gcccgcttcg atccggtggc gatgcacgtg gggatcaaca
     6301 ccgggcccgc cctcctcggc gcgaccaaga tcgagggcag cgcgggcacc cgctggacgt
     6361 ataccgcctc cggcatgacc accaacatcg cggcgcgcct gtccgcgcag gccggcgacg
     6421 gcgagatcgt gctcagcgag ccgacccggc ggcgcctgga cgccgacctg gccgtcgagg
     6481 acctgggcgt gcgcgagctc aagaacgtgg acaccccgtt gcggctcttc cggctgcggt
     6541 agcgcggccc gccgggcgtt tttcggccgg cgggcgtctt gacgggcggg gggctgcgcc
     6601 tatagaatga cgcaccgcga tcgaccgccg acgtccggcg cggagtcccg acggatccct
     6661 gaagtccatc gaacccgagg aggagccaac acaatggaac tgacccgacg cgacctgctc
     6721 aagggggcgg cagccctggg cgccgccgcc gcggtgccgc tgactgccgg tggccccgcg
     6781 ctggcgcaga ccacccagaa gaaggagctg atcaccgccc agagcggcga cgtctcgaag
     6841 ttcgacccgc actacagcac ctcgtcgaac gacgtccgcg tctcgttcaa cctcttcgac
     6901 aacctcacga gccgccatcc ggacggcaag ctctacccgg ggctcgccac cgagtggaag
     6961 ctcgaggggc cgaccacgtg gcgcttcaag ctgcggcagg gggtgaagtt ccacaacggc
     7021 gacccgctca cctcggccga cgtcaagtac agcatcgagc gcacctacga tccggcggcc
     7081 aagacgatgg tggcgaccgt cttcggcacc atcgagaagg tcgacgcgcc cgacgcctcc
     7141 acgatcgtga tcaccaccaa gaagccggac ccgctgctcg cggcgcggct cgccttctac
     7201 ggcgggcagg tggtgccgaa gaagtacctc gagtcggtcg gcaacgacgc cttcaacgcc
     7261 aagccgatcg gctcgggccc ggtgcgcttc acctcctggg tgaaggacga ccgcgcgatc
     7321 ctcgaagcca atccggacta ctggggcggc aagatcgaca tggaccgctg gatcatgcgg
     7381 ccgatcccgg agaccgcgcc gcggatcgcg gcgctgctga agggcgaggt cgacgcgatc
     7441 acccagctcc cgcccgacca ggaagatcgg gtcgcggcca acccgaccac ccgggtcgcg
     7501 ggggcgctct acgcgggcct ctacgtgctc gcggtgaaca gcaagcggcc gccgctggac
     7561 aatccgctcg tgaagcaggc gctgtcgctc gccatcgacc gcgaggcgat cgtgaaggag
     7621 ctgtggcgcg gccgcggcgt ggtgccgaac ggtcccatcg ccaagggcga caaccacttc
     7681 gacgcgagcc tgcccccgct ggcctacaac ccggcggagg cgaaggcgcg gctccagaag
     7741 gcgggctaca agggcgagga ggtcttcatc gagaccaccg tcgcctacgt ggcccaggac
     7801 aaggcgatgg cggaagccat cgcggccatg tggaaggacg tcggcgtcaa cgtgaaggtc
     7861 gaggtcatcg agtactcggt gcgggcgcag aagaaccgcg agaagagctt caagggcgtg
     7921 ttctggtccg atccgacgtc gaccctgagt gacccggacg ggatgatgtg gcgcctcctg
     7981 gggccgggcg ggccacagga ctactggcgc gaggccaagt tcgacgagct gggcaacgcg
     8041 gcgcgcttca gcgtggacga gaagttccgc gggcagtcct acaaggagat gaccaagatc
     8101 ttcctggagc acctgccgtg gctgccggtc atccagccct acgaggacta cggcatccag
     8161 aagtacatcg acttcacgcc gaacccgaac cagcagttcg agatccgacg cttcaacttc
     8221 aagt
//
LOCUS       DATMCV010000697         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_973316, whole genome shotgun sequence.
ACCESSION   DATMCV010000697 DATMCV010000000
VERSION     DATMCV010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973316"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VNU03_15895"
     CDS             <1..802
                     /locus_tag="VNU03_15895"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWP76006.1"
                     /translation="EDNLRALDETARLQRLRVDRGDLSELELLRIQVQRFTFERDAAD
                     ARQALDVARITLRTTAGPAAIAEDFEVAGDLQFADVSLDPDRLRRLTVANRPDLRAAM
                     AERERAQADHRLARANAWWDITPQIEYQRIGPDNTIGFGFSFPLRVFDRNQGEIERTR
                     SEIARVEATRQATEQQALAGLDVDLRTAQTQRERVLVLRDVYLPKARQTREVVEYAYR
                     RGGQSLLDFLDAQRSYRETALALLQATGAYLSAIYQLEADVGGPLDQP"
     gene            902..1732
                     /gene="argB"
                     /locus_tag="VNU03_15900"
     CDS             902..1732
                     /gene="argB"
                     /locus_tag="VNU03_15900"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWP76007.1"
                     /translation="MGESDPVATGARKTVVVKYGGAAVERGRRLDGFLRDIAALRSAG
                     IDLVVVHGGGPQISRLMEESGKTPRFVGGMRVTDDETMELVERALRQVNDEIAQMINR
                     HGARAVGLSIWPGPLIHACHRMHLLPTGESVDLGRVGDVAGVNTTPIRALLERGVVPV
                     IGPIGVGADHRTYNINADLVAGEVAAVMGAALLIYLTDVPGIVDRSGGRVRRLSRWDA
                     DCLVRDGVIDGGMLPKIEGAVRALKGGAAQALIVDGRIPHALRLTLRAPHHVGTEIVL
                     "
     gene            complement(1748..>2079)
                     /locus_tag="VNU03_15905"
     CDS             complement(1748..>2079)
                     /locus_tag="VNU03_15905"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWP76008.1"
                     /translation="VKSFGGGGGIGKDIPGTTFTYGLVNYTMFETSRKSFFTVRNEWW
                     RDEDGERSGFASNYSSHTVGFTYQLSPSIMVRPEVGYYHSYNAKAFDLGKKDYAVIGG
                     LDVTLRF"
BASE COUNT          316 a          715 c          733 g          315 t
ORIGIN      
        1 cgaggacaat cttcgggctc tcgacgagac cgcgcgactg cagcggctcc gggtcgaccg
       61 gggcgacctg tccgagctgg agctgctccg catccaggtg cagcgcttca ccttcgagcg
      121 cgatgcggcc gacgcccggc aggccctcga cgtggcccgg atcaccctgc ggaccaccgc
      181 cgggcccgcc gccatcgcgg aagacttcga ggtcgccggc gatctccagt tcgccgacgt
      241 ctccctcgac cccgatcgtc tccggcgtct gaccgtcgcg aaccggcccg acctgcgcgc
      301 ggcgatggcg gagcgggagc gcgcccaggc ggatcaccgg ctcgcccggg ccaacgcctg
      361 gtgggatatc acgccccaga tcgagtacca gcgcatcggc ccggacaaca ccatcggctt
      421 cggcttctcc ttcccgctgc gcgtcttcga ccgcaatcag ggcgagatcg agcggacccg
      481 gtccgagatc gcccgggtgg aggccacccg ccaggcaacc gagcagcagg cgctcgccgg
      541 gctcgacgtc gacttgcgaa ccgcgcagac ccagcgcgag cgggtgctcg tcctgcggga
      601 cgtctacctg ccgaaggcgc ggcagacgcg cgaggtcgtg gaatacgcgt accggcgggg
      661 cggacagagt ctgctcgact ttctcgacgc gcagcgcagc taccgggaga cggccctcgc
      721 cctccttcaa gccactggcg cgtatctgtc cgccatctac cagctcgagg ccgacgtggg
      781 cgggccgctc gatcagcctt gacgcttcct tgacgacccg gaccgggcat tggtcgcctt
      841 cttgacctga cctgcgcatc caatcgggtg aaggatgcaa acccgagggg agggcacggt
      901 catgggcgag agcgacccgg tcgcgacggg cgcgcggaag acggtggtcg tcaagtacgg
      961 cggggcggcc gtggagcgtg gtcggcggct ggatgggttc ttgcgggaca tcgccgcgct
     1021 ccggtcggcc gggatcgacc tcgtcgtggt ccacggtggt ggcccccaga tcagccggct
     1081 gatggaggag tcgggcaaga ccccgcggtt cgtcggtggc atgcgcgtca ccgacgacga
     1141 gaccatggag ctcgtcgagc gggccctgcg gcaggtcaac gacgagatcg cgcagatgat
     1201 caaccggcac ggcgcccgcg ccgtggggct gagtatttgg ccgggtccgc tcattcacgc
     1261 ctgccaccgg atgcatctcc tgccgaccgg cgagtccgtg gatctcgggc gggtcgggga
     1321 cgtggcgggg gtgaacacga ctccgatccg ggcgctgctg gagcgcggcg tggtccccgt
     1381 catcggaccg atcggagtcg gagccgacca tcgcacgtac aacatcaacg cggatctggt
     1441 ggccggcgag gtcgcggcgg tgatgggcgc ggccctcctg atctatctca cggatgtgcc
     1501 cgggatcgtg gaccgctccg gcggccgagt ccggcgtctc agccgctggg acgccgactg
     1561 cctcgtgcgg gacggggtga tcgacggcgg gatgctgccg aagatcgagg gcgcagtccg
     1621 ggcgctgaag ggcggcgccg cccaggcgct gatcgtcgat ggacggattc cgcacgcgct
     1681 gcgcctgacg ctccgggcgc cgcaccacgt gggcaccgag atcgtgctct agggcgccga
     1741 ggctcgacta gaaccgcagc gtcacgtcca gccccccgat gaccgcgtag tccttcttgc
     1801 cgagatcgaa cgccttcgcg ttgtaggagt ggtagtagcc cacctcgggc ctcaccatga
     1861 tcgacgggct gagctggtag gtgaagccga cggtgtggct cgagtagttg gacgcaaagc
     1921 cggatcgctc gccgtcctca tcccgccacc actcgttgcg caccgtgaag aagctcttcc
     1981 gcgaggtctc gaacatcgtg tagttcacga gcccatacgt gaaggtcgtg cccgggatgt
     2041 ccttcccgat gccgcccccg ccgccgaagg acttcaccg
//
LOCUS       DATMCV010000698         4014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_440315, whole genome shotgun sequence.
ACCESSION   DATMCV010000698 DATMCV010000000
VERSION     DATMCV010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440315"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VNU03_15910"
     CDS             <1..925
                     /locus_tag="VNU03_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76009.1"
                     /translation="CAAGVRFGVQESRPAPHAVHSYRTDYPSIGTVAARAGSSGPPPE
                     PSTAHTDRRPAEYSLCHAAGTAEIDLRLFVARVDAQMPKPGYEHEAPLNSSFFEYSGN
                     VYDPGYDPDRSWPSPEAIRSAPAVFVGVPGSERGTFFGYNIPTPAALYASEFFTEWNP
                     VKPVGRSTAFGVDVALGLFLGYVFAFTWERFARAEERLQETRLFPLARSVGRKAWRYL
                     VARGLLLANLALLAGVLAGHVLVSGQLLQSRNWLNPLPIVLGTCIDGLLHSRTRSDPI
                     EPQSWRAFVTRRSDALAQVVIIFWALWHLLS"
     gene            1007..2428
                     /gene="hydA"
                     /locus_tag="VNU03_15915"
     CDS             1007..2428
                     /gene="hydA"
                     /locus_tag="VNU03_15915"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HWP76010.1"
                     /translation="MAEFDTVIKNGLVVTAADVVEADLGIAGGVVTAVGRSLGPARRE
                     IDAAGRYVMPGGIDTHCHFDQPMRDSVTLADDFLSGTVSAAHGGTTTVVPFACQLQGR
                     SLREAVDDYHRRAAAKPVIDYAFHLIISDPTEAALRDELPALIREGYSSFKIYMTYET
                     LKLSDRQIIAVLAVARREGAMVMVHAENSDCIAWLTEELERQGKTAPYFHAVSRPQAV
                     EREGTHRAITMSEIVGAPILLVHVSSADAVEQIRWAHGRGLHILAETCPQYLVLTAEH
                     LHAPGFEGAKYICSPPPRDEASQRAVWHGLASGVFQVVSSDHAAFRFDDPAGKKRHGD
                     RASFTQVPNGIPGVETRMPLLFSEGVGKGRIDLTTFVALTSTNAAKIYGLYPRKGTIA
                     VGSDADLVVWDKDREVVITSGLLHSSCDYTPYEGMAVRGWPAVVLSRGEVVVEEGKLL
                     ATPGRGQFLRCDRPQPVFSNLGP"
     gene            2614..3804
                     /locus_tag="VNU03_15920"
     CDS             2614..3804
                     /locus_tag="VNU03_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76011.1"
                     /translation="MKTARAWLALTIVTVALGALALGAGPAAAADPVKIGLVAALSGQ
                     SAASGEAITRGLTVAIEEINGKGGVLGGRMLELVRRDDESNPSKGVTAARELIFKEQV
                     AALFGGIDSPVSLAIVPIANAEKKPFLGVWAAATGITRNGANPNYVFRVSAVDVIVDK
                     GLIQYAAKTYGAKKPGLMLINNPWGESNNDGLTKAAAAQGLTLAGVEKFQTEDVDMVP
                     QLSRLKEAGADSIILVANAAPAAQVMRSLDRMGWPVPVISHWGISGGRFVELGGPGAT
                     KVHFVQTYSFFGEQNPRGKHLLELLMKRYPAVKGPADVIAPVGTANAYDAMHILARAI
                     QSAGATDGPKVREALLKAGRYEGLIKTYDPAFTADNHDALNENDYVMVRYTEKGEIVP
                     VKGK"
     gene            3833..>4014
                     /locus_tag="VNU03_15925"
     CDS             3833..>4014
                     /locus_tag="VNU03_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017798994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76012.1"
                     /translation="MLILSALITGVATGSMYGLLALGFHVTWAVSRTVNFAQGSSMML
                     GAVLLYGLAVTHHWPGA"
BASE COUNT          600 a         1433 c         1381 g          600 t
ORIGIN      
        1 ctgcgccgcc ggcgtccggt tcggcgtgca ggagtcccgg cccgctccgc acgccgtcca
       61 cagctaccgg acggactacc cgtcgatcgg caccgtcgcc gcgcgggccg gctcgtcggg
      121 accgccgccc gagccgagca ccgcgcacac cgatcgccgg ccggcggagt acagcctgtg
      181 ccacgccgcg ggaaccgccg agatcgacct tcgcctgttc gtggcccgcg tggacgcgca
      241 gatgccgaaa cccggctacg agcacgaagc tccgctgaac agctcgttct tcgagtacag
      301 cgggaacgtc tacgatcccg gctacgatcc cgaccggtcc tggccctcgc ccgaggcaat
      361 tcgaagcgcg cccgcggtgt tcgtcggggt accggggtca gagcgcggaa ccttcttcgg
      421 atacaacatc ccgaccccgg cggccctcta cgcgtcggag ttcttcacgg aatggaaccc
      481 ggtgaaaccg gtcggccggt cgaccgcgtt cggcgtcgac gtggccctgg ggctcttcct
      541 cgggtacgtg ttcgccttca cgtgggagcg gttcgcgcgg gcggaagagc ggctgcagga
      601 gacccggctg ttcccgctcg cccgcagcgt cgggcgcaag gcgtggcgct acctggtggc
      661 ccgcgggctc ctcctcgcca atctcgctct tctcgccggc gtgctggccg gccacgtgct
      721 cgtgtccggt cagctgctgc agtcacggaa ctggctgaac ccgctgccga tcgtcctcgg
      781 gacgtgcatc gacgggctcc tgcacagccg gacccggtcg gacccgatcg agccccagag
      841 ctggcgggcc ttcgtgacga ggcgctccga cgctctggcc caggtcgtga tcattttctg
      901 ggcgctctgg cacctcctga gctgaccgtt gcgctcgaga ccccttgcag accggtcgtc
      961 gcccggtcat cctcggcgcg tcgccatcgg aaggagggct cagctcatgg cggagttcga
     1021 cacggtcatc aagaacgggt tggtggtcac cgcggccgac gtcgtcgagg ccgatctcgg
     1081 catcgcgggc ggtgtcgtca ccgcggtggg ccggagcctc ggcccggcac gccgggagat
     1141 cgacgcggcg ggacgctacg tgatgcccgg cgggatcgac acgcattgcc acttcgacca
     1201 gcccatgcgc gactcggtca ccctggccga cgacttcctg tcgggcacgg tgtcggccgc
     1261 ccacggaggc acgaccaccg tcgtgccctt cgcctgccag ctccagggac ggagtctccg
     1321 ggaggcggtc gacgactacc accggcgggc cgcggccaag ccggtgatcg actacgcctt
     1381 ccacctcatc atctccgacc cgaccgaggc cgcgctgcgc gacgagctgc cggcgctgat
     1441 ccgggagggc tactcctcgt tcaagatcta catgacctac gagaccctca agctgagcga
     1501 ccggcagatc atcgcggtgc tcgcggtggc gcggcgcgag ggggccatgg tgatggtcca
     1561 cgccgagaac tcggactgca tcgcgtggct caccgaggag ctcgagcgac agggcaagac
     1621 cgcgccctac ttccacgcgg tctcgcgtcc gcaggcggtg gagcgcgagg gcacccaccg
     1681 cgcgatcacc atgtcggaga tcgtgggcgc gcccatcctg ctggtccacg tctcgagcgc
     1741 ggatgcggtc gagcagatcc gctgggccca cggtcgcggc ctccacatcc tggccgagac
     1801 ctgcccccag tacctcgtcc tcaccgccga gcacctgcac gcgcccggtt tcgagggcgc
     1861 caagtacatc tgcagcccgc ccccgcgcga cgaggcgagc cagcgggcgg tgtggcacgg
     1921 ccttgcctcg ggtgtcttcc aggtggtctc gtccgaccac gcggccttcc gcttcgacga
     1981 tccggccggc aagaagcggc acggcgaccg cgcctccttc acccaggtgc ccaacggcat
     2041 ccccggcgtc gagacgcgga tgccgctgct cttctccgag ggcgtgggca agggccggat
     2101 cgatctgacc acgttcgtgg cgctgacctc gaccaacgcg gccaagatct acggcctcta
     2161 cccgcggaag gggacgatcg cggtcgggtc cgacgccgac ctggtcgtgt gggacaagga
     2221 ccgcgaggtc gtcatcacca gcggcctcct gcactcgagc tgcgactaca cgccctacga
     2281 gggcatggcg gtgcgcgggt ggccggccgt cgtcctctcg cggggcgagg tggtggtcga
     2341 ggagggcaag ctgctggcca cacccggtcg cggccagttc ctgcgctgtg atcgccccca
     2401 gcccgttttt tccaacctcg ggccctgacc tgggcgacgg atgctccggc gacggatgct
     2461 ccttcggggg ccaccgggat ctccggttgc ccagaaattg ggcaaccggt cgcggcgtcg
     2521 atgatctaaa gtagcgattc ccttcagggc tcgccgtggc acacggggtg cagtttcccg
     2581 cccggcgaaa tcccactcca aggagatcag gatatgaaga cggcgcgcgc gtggctcgct
     2641 ctcacgatcg tcacggtggc tctcggggcg ttggccctcg gagcgggccc ggccgccgcc
     2701 gcggacccgg tgaagatcgg cctggtggcc gccctctccg gccagtccgc ggcctcgggt
     2761 gaggcgatca cccgcgggct cacggtggcc atcgaggaga tcaacggcaa gggcggcgtg
     2821 ctcggcgggc gcatgctcga gctggtccgg cgcgacgacg agtcgaatcc gtccaagggc
     2881 gtgaccgcgg cgcgcgagct catcttcaag gaacaggtgg cggcgctctt cggtggcatc
     2941 gactcgccgg tgtcgctcgc catcgtgccg atcgccaacg cggagaagaa gccgttcctc
     3001 ggcgtgtggg cggcggccac cggcatcacc cgcaacggag ccaacccgaa ctacgtgttc
     3061 cgcgtgtccg cggtcgacgt gatcgtcgac aaggggctca tccagtacgc ggcgaagacg
     3121 tacggggcca agaagcccgg cctcatgctg atcaacaacc cgtggggcga gtccaacaac
     3181 gacgggctca ccaaggcggc ggccgcgcag gggctgaccc tcgcgggcgt cgagaagttc
     3241 cagaccgagg acgtcgacat ggtgccgcag ctctcgcggc tcaaggaggc gggcgccgac
     3301 agcatcatcc tggtggccaa cgcggcgccg gccgcccagg tcatgcgctc gctcgaccgc
     3361 atgggctggc cggtaccggt gatctcgcac tggggcatct cgggcgggcg cttcgtggag
     3421 ctgggcgggc cgggcgcaac caaggtgcac ttcgtccaga cctacagctt cttcggcgag
     3481 cagaacccgc gcggcaagca cctgctcgag ctgctgatga agcgctatcc ggccgtgaag
     3541 gggcccgccg acgtcatcgc cccggtgggc acagccaacg cctacgacgc catgcacatc
     3601 ttggcccggg ccatccagtc cgccggcgcc accgacggcc ccaaggtgcg ggaggcgctg
     3661 ctcaaggccg gccgctacga ggggctgatc aagacctacg atcccgcgtt caccgcggac
     3721 aaccacgacg cgctcaacga gaacgactac gtgatggtcc gctacaccga gaagggcgag
     3781 atcgtcccgg tgaagggcaa gtagcggcgc ggccccgcgg cggcagcggc cggtgctgat
     3841 cctctccgcc ctgatcaccg gcgtcgccac cgggagcatg tacgggctcc tcgcgctggg
     3901 cttccacgtg acctgggcgg tgtcgcggac cgtgaacttc gcgcagggca gctcgatgat
     3961 gctgggcgcg gtgctcctct acgggctcgc ggtcacccac cactggcccg gcgc
//
LOCUS       DATMCV010000699         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_503961, whole genome shotgun sequence.
ACCESSION   DATMCV010000699 DATMCV010000000
VERSION     DATMCV010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503961"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VNU03_15930"
     CDS             <1..377
                     /locus_tag="VNU03_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWP76013.1"
                     /translation="QYVNTTKEIILGRMLGDYDYGDGRKEKDKYYMTFFDRQTTFPLK
                     SHGVWWLSQFRRWGMVKEPPDYKAVVDRVHRPDIYREVAREMSLETPREDMRKETLFD
                     GVTFDPAEPEKYAKGFPIHSMS"
     gene            393..1163
                     /gene="ntrB"
                     /locus_tag="VNU03_15935"
     CDS             393..1163
                     /gene="ntrB"
                     /locus_tag="VNU03_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HWP76014.1"
                     /translation="MSDGWVMRIVLPCLGIACVLALWAVVSQTVAPDLPSPLKTWQES
                     RRYVLEPFFKDGEMNQGIGRLALYSLERVGKGYLLALAIGTPLGFLLGLSHRFHESFD
                     PIIQFLRPISPLAWLPLGLVVFRRSEPAAIFTIALCAMWPTVINTAVGVRGISPDYLN
                     VGRVLRLSRRKMLTKIIVPAALPYVFTGYRLSLGLAWLVIVAAEMLTGTPGVGGFLWQ
                     EYNSLVYSHIILSVITIGAIGFVLDRLMGLAEARFKAT"
     gene            1163..1945
                     /locus_tag="VNU03_15940"
     CDS             1163..1945
                     /locus_tag="VNU03_15940"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76015.1"
                     /translation="MSYLEVRGIAKSFVTPRGTTRVLGGVDLAVEEGEFVAIVGYSGS
                     GKTTLISLIGGLLAPDAGRITLEGAAVVEPGPERAIVFQQYSLLPWMTVYDNVALAVD
                     AVNPDRSPRERRRLTEELIDLVHLTPAVWKRPRELSGGMRQRVAVARGLAMRPKVLLM
                     DEPFSALDALTRATLQDELLRVWGERRSTVMLVTNDVDEAILLSDRIYPMTPGPGAVL
                     GPPIEVGLARPRERRHMSLSPAYQKARQGLVELLRGCRRPTP"
     gene            1950..>2586
                     /locus_tag="VNU03_15945"
     CDS             1950..>2586
                     /locus_tag="VNU03_15945"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76016.1"
                     /translation="MAAFVEIALLGKTYPTPSGPAEIVRDVTLNVARGEFVCIVGHSG
                     CGKSTVLSILMGLNAPTTGGVVIAGREVSGPGIDRGVVFQSATLLPWLSVRDNVKLAL
                     DQVPRPGRARDPEPFLAAVGLGGQGERYPRELSAGMQQRVGIARAFALEPRLLLLDEP
                     FSLLDALTRMELQDELMTLWEAERKTVIMVTHDVDEALLLADRVVMMTNGPA"
BASE COUNT          364 a          899 c          905 g          418 t
ORIGIN      
        1 cccagtacgt caacaccacc aaggagatca tcctgggccg catgctcggg gactacgact
       61 acggcgacgg gcgcaaggag aaggacaagt actacatgac cttcttcgat cgccagacca
      121 cctttcccct gaagtcgcac ggggtctggt ggctgagcca gttccgccgc tggggcatgg
      181 tgaaggagcc gcccgactac aaggccgtcg tcgaccgcgt gcaccggccg gatatctacc
      241 gcgaggtcgc ccgcgagatg agcctcgaga cgccccggga ggacatgagg aaggagaccc
      301 tgttcgacgg ggtcaccttc gacccggcgg agcccgagaa gtacgcgaag ggcttcccga
      361 tccattccat gagctagggg cttggagacg acatgtccga cggctgggtg atgcggatcg
      421 tgctgccgtg cctggggatc gcgtgcgtgc tcgcgctgtg ggcggtggtc agccagacgg
      481 tggcgcccga cctgccgtcc ccgctcaaga cgtggcagga gtcccggcgc tacgtgctgg
      541 agccgttctt caaggacggg gagatgaacc agggcatcgg gcgtctcgcc ctctactcgc
      601 tggaacgggt gggcaagggc tatctgctcg ccctcgcgat cgggaccccg ctcgggttcc
      661 tgctgggcct ctcccatcgc ttccacgaat cgttcgatcc catcatccag ttcctgcggc
      721 cgatctcgcc gctcgcgtgg ctgccgctgg ggctggtggt gttccgccgg tcggagccag
      781 ccgccatctt cacgatcgcc ctctgcgcga tgtggcccac cgtcatcaac accgcagtgg
      841 gcgtgcgcgg gatcagcccc gactacctga acgtgggacg ggtgctcagg ctgtcgcggc
      901 gcaagatgct caccaagatc atcgtgcccg ccgcgctgcc ctacgtgttc accggctatc
      961 gcctctccct cggcctcgcg tggctcgtca tcgtcgccgc cgagatgctg accggcaccc
     1021 cgggggtggg cggcttcctg tggcaggagt acaacagcct cgtctactcg cacatcatcc
     1081 tgagcgtcat caccatcggg gcgatcggct tcgtcctcga tcgcctcatg ggcctggcgg
     1141 aggcccgctt caaggccacc tgatgtcgta cctcgaggtt cgagggatcg cgaagtcgtt
     1201 cgtcacgccg cgcggcacca cccgggtgct gggcggcgtg gatctcgcgg tggaggaggg
     1261 cgagttcgtc gcgatcgtgg ggtactcggg ctcgggcaag accacgctga tctcgctgat
     1321 cggaggcctg ctcgcccccg acgcgggccg catcaccctg gagggggctg cggtcgtcga
     1381 gcccgggccc gagcgcgcca tcgtcttcca gcagtactcg ctgctgccct ggatgacggt
     1441 gtacgacaac gtggctctcg cggtggacgc ggtcaatccg gaccggtcgc cccgcgagcg
     1501 gcggcggctc accgaggagc tcatcgacct ggtgcatctc acccccgcgg tgtggaagcg
     1561 cccgcgcgag ctgtcgggcg gcatgcgcca gcgggtggcg gtcgcccgcg gcctcgcgat
     1621 gcggccgaag gtgctcctca tggacgagcc cttcagcgcg ctggacgcgc tgacccgcgc
     1681 caccctgcag gacgagctgc tgcgcgtctg gggcgagcgc cgctccaccg tgatgctggt
     1741 caccaacgac gtggacgagg cgatcctgct ctccgatcgc atctatccga tgacgccggg
     1801 ccccggcgcg gtgctgggcc cgcccatcga ggtggggctg gcgcggcccc gcgagcggcg
     1861 ccacatgagc ctgagcccgg cctaccagaa ggcgcgccag ggcctcgtgg aattgctccg
     1921 cggctgtcgc cgcccgacgc cctgacgcga tggcggcgtt cgtcgagatc gcgctcctgg
     1981 gcaagacgta cccgaccccg tcggggccgg ccgagatcgt gcgcgacgtc accctcaacg
     2041 tggcgcgggg cgagttcgtc tgcatcgtcg gccattcggg ctgcgggaag tccaccgtgc
     2101 tctccatcct gatgggcctg aacgcgccca ccaccggcgg agtggtgatc gcggggcgcg
     2161 aggtgagcgg gcccggcatc gaccgggggg tggtcttcca gtccgcgacc ctgctgccct
     2221 ggctgtcggt gcgcgacaac gtgaagctcg cgctcgacca ggtcccgcga cccgggcgcg
     2281 cccgcgaccc cgagccgttc ctcgccgccg tcgggctggg cgggcagggg gagcgctacc
     2341 cgcgcgagct ctcggccggc atgcagcagc gggtgggcat cgcccgtgcc ttcgcgctgg
     2401 agccgcggct cctgttgctc gacgagccgt tctcgctgct cgacgcgctg acccgcatgg
     2461 agctgcagga cgagctgatg accctgtggg aggccgagcg caagaccgtc atcatggtca
     2521 ctcacgacgt cgacgaggcg ctgctcctgg ccgaccgggt ggtgatgatg acgaacggcc
     2581 ccgccg
//
LOCUS       DATMCV010000700        10211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_171356, whole genome shotgun sequence.
ACCESSION   DATMCV010000700 DATMCV010000000
VERSION     DATMCV010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171356"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /gene="miaA"
                     /locus_tag="VNU03_15950"
     CDS             <1..865
                     /gene="miaA"
                     /locus_tag="VNU03_15950"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWP76017.1"
                     /translation="PIEVVSADSRQVYRGLDIGTGKPSAEERAAVPHHLLDVADPGER
                     YNAARFRTDARAAIAAIHGRGRLPVVVGGTGLYVRALLKGLTPAPPADPALRAELEAM
                     AQAQGSAALHARLAALAPEIARRLHPNDRVRVVRAIEIHAGSATAPAEGAEGDWSGAT
                     RAWRLLMIGLGRERAALNAAIALRARAMLARGMMSEVERLLATGHDPERPPMDGIGYR
                     PLALAARGRLPVEEALRLMIRDTTRYAKRQMTWFARDPEIRWIDVDAAGGVEGAAETV
                     SKHLTQEGLTE"
     gene            862..2112
                     /gene="hflX"
                     /locus_tag="VNU03_15955"
     CDS             862..2112
                     /gene="hflX"
                     /locus_tag="VNU03_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWP76018.1"
                     /translation="MKSREKAVLAAVRLPQQPRVVVEESLDELGRLAESAGALVVGRA
                     TQDRRAPTPGLYFGKGKVEEIKTLSKREGANLMISDDPLSPIQERNLGGSLGVRVIDR
                     TALILDIFAQRARTMEGKLQVELAQLSYLLPRLVGQWKHLERTGGGIGTRGPGEMQIE
                     SDRRMIRHRIQKIRDELQRVRVHRRLLRDRRKASGVPVVALVGYTNAGKTTLLNHLTG
                     ADRVAADALFVTLDPTARLVTSSTHASFILTDTVGFIRKLPHQLVAAFKATLEELTEA
                     DVLIHVVDASHPGLDDQVQAVEALLGELELMGRPMIVALNKIDRLESGAARSLLSRFD
                     GVAISARTGEGMEALRERVDLALSPRVARLTLRIPYADGPALAACYERGRVLTRRDEP
                     EGIRVEVEMPRRLMASFEGYRLPG"
     gene            2155..2709
                     /gene="pgsA"
                     /locus_tag="VNU03_15960"
     CDS             2155..2709
                     /gene="pgsA"
                     /locus_tag="VNU03_15960"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWP76019.1"
                     /translation="MGLPNWLTTLRILLIPVFVTLLVYRRAGAALMVFCLASLTDLLD
                     GYVARVQGRQTRLGAFLDPVADKLLLTSGFVTLTWLKVLPFWITAVVVSRDVVLSIGV
                     LVIHVAGGTVHPSPTWVGKMSTVFQMALVLAAMAGAYFQLVPPFVTKLLAWVTAGFTI
                     TSGLQYIVMGLKQLETPTSVGGRL"
     gene            2712..4052
                     /locus_tag="VNU03_15965"
     CDS             2712..4052
                     /locus_tag="VNU03_15965"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HWP76020.1"
                     /translation="MPRRTAATAEGVVVAAVAPRTAAARAGLRPGDRIVAINGAPLRD
                     VIDFHFHAGEETLDLALDREGRERTVRMTRRGAALGLELAPPRPAEIATCANKCVFCF
                     IHQLPKGMRKSLYVKDDDFRLSFLHGNYITLTDLDEAELQRIETQRLSPLYVSVHATD
                     PDLRHALLGQPRLRRELLPVMERLAKAGIAMHAQIVLCPGWNDGAHLERTVHELARLH
                     PAVPTTAVVPVGLTRHRERLPDLRAVTPGEAATLVRDIEGWQRAFQATLGTRFVWAAD
                     ELYLGAGLPLPPARAYEGFAVAEDGIGLVRRFEDAWARALARPPAPLTRLRRATVVTG
                     ELFAPRMRALLDRLAVPGLTVDLVPIRNEFFGRGIGVAGLLTGRDIQLQLGEAGCGGR
                     GLGDEVLVPAVTLRDGAGVFLDDLTPADLGAALGTPVTPVEPTAPAFLAALVGR"
     gene            4080..5429
                     /gene="der"
                     /locus_tag="VNU03_15970"
     CDS             4080..5429
                     /gene="der"
                     /locus_tag="VNU03_15970"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWP76021.1"
                     /translation="MLRPIIAIVGRPNVGKSTLFNRLIGRRRSLVRDVPGVTRDRLYG
                     TAVHERWQATVVDTGGFDPSSESDLVQGVRRQVLQAVDEADLLVFVVDGRAGVSGLDE
                     EIAGILRKSNRPVLLAVNKVDGAEQESPTGDAYRLGFTPVLTISAEHGRGVAELLEAC
                     RDLAPAVLPETGTEGTRVAIIGRPNVGKSSLVNAVLGHERVLVHDQPGTTRDAVDTPL
                     SFRGRDYLLIDTAGIRRKGKVTEPLEKLAVVMALKSLERCQVAVLVVDASEGVAAQDA
                     HIAGYANDAGRATVVAVNKWDLVPPGLVTKGDVTTQIYERLPFLEHAPICFTSAVVGF
                     GLRDLFDQVDLVAAEAQKRLQPGEVLSTLRQAVERRPMSSRGVPLRVQSAQQVAIGPP
                     TFALRVNLPDQIHFSYERYLINSLRHSHSFAGSPIRLLFRKAVGRGAKRTRRAPRRA"
     gene            5575..6297
                     /locus_tag="VNU03_15975"
     CDS             5575..6297
                     /locus_tag="VNU03_15975"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWP76022.1"
                     /translation="MSPERPEDDDFLEDEEQEEYGQRSIFATGWFRAVLVLLVLAVIA
                     VIAVPLIGGWFEPTPPPKVTTTRPVEPTPAPATPAPTPPPVTPAPVTPPPVAQLPTPA
                     PTAPPATPAPVTPPPAVSTPPARPEMPAASTPSRAAEKAKAPERTASASSGKSASKAP
                     AANGSYWVQVGAFKESKNAEGLARTLRSEGFTVQVATVTRGEPLHVVRVGGYADRGKA
                     TAAREELQGKGHSGFVMQGPAK"
     gene            6321..6593
                     /locus_tag="VNU03_15980"
     CDS             6321..6593
                     /locus_tag="VNU03_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HWP76023.1"
                     /translation="MTKNDLINAVAAHGLSKRQSASVVESLFDIIFRCFEKGEDVKIV
                     GFGHFRIRQKASRRGRNPQTGDSIEITARKVLTFKPSKGLKLRINV"
     gene            6612..6983
                     /locus_tag="VNU03_15985"
     CDS             6612..6983
                     /locus_tag="VNU03_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWP76024.1"
                     /translation="MSTVPDKLYYRIGEVETITEIPAYVLRYWESEFKLLRPKKNPAG
                     QRLYRKADLELVLRIKSLLYEERLTLEGAKKRLRSESRGLDQMDLGLRGATLEDALKR
                     TRSRLQAIRQRLAAPRSGRSS"
     gene            7020..7096
                     /locus_tag="VNU03_15990"
     tRNA            7020..7096
                     /locus_tag="VNU03_15990"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7054..7056,aa:Pro,seq:ggg)
     gene            7109..7891
                     /gene="surE"
                     /locus_tag="VNU03_15995"
     CDS             7109..7891
                     /gene="surE"
                     /locus_tag="VNU03_15995"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWP76025.1"
                     /translation="MAPVILVTNDDGIHARGLAVLAEALAEIGEVYVVAPDREQSAVG
                     HALTLHRPLRVDRVAERRYAVNGTPSDCVNLAVLGLLPEPPVLVASGINHGTNLGDDV
                     TYSGTVSAAMEGTLLGVPSMAVSQADPEAEGGFEAAGAVARAVAMRLLVEGLPAKTLL
                     NVNVPRGEIKGIRLTRLGHRVYREKVIREVDPRGRPYYWIGAGPPEWAEDASADIAAM
                     HEGWASVTPLHLDLTHHGALGRMTEWEGALNAVLKKKKKRSR"
     gene            7888..8562
                     /locus_tag="VNU03_16000"
     CDS             7888..8562
                     /locus_tag="VNU03_16000"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWP76026.1"
                     /translation="MTGGAETKAGSARFARERERMVEEQVIARGVRDPRVIEVMRRLP
                     RHVFVDEALRDRAYGDHPLPIGEGQTISQPFIVGRMTELLRLGGREKVLEVGTGCGYQ
                     AAVLAELAGRVCTMERIPRLASRARETLEGLGYRNVWVRAANGTLGWPDEAPFDRILV
                     AAGGPGVPPPLFEQLAEGGRMVIPVGEVDGQVLQVIEKVQGTMRVTPDSGCVFVKLVG
                     KYAWES"
     gene            8587..8663
                     /locus_tag="VNU03_16005"
     tRNA            8587..8663
                     /locus_tag="VNU03_16005"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8621..8623,aa:Pro,seq:cgg)
     gene            8706..9011
                     /locus_tag="VNU03_16010"
     CDS             8706..9011
                     /locus_tag="VNU03_16010"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWP76027.1"
                     /translation="MGITGLRSAAEIVVEGRVQGVGYRDYVQRKAASLGLAGYVMNLK
                     NGKVRVRVEGGRDLIEELMHDLEKGPPLSRVDHLTVTWRPLTGRYTGFSIRYAEFDP"
     gene            9008..9970
                     /locus_tag="VNU03_16015"
     CDS             9008..9970
                     /locus_tag="VNU03_16015"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWP76028.1"
                     /translation="MRHGPARARRAIGRALLLPILLVPTALAVASVSHAQTKKAIDAT
                     PTPAKTSPREPTSTRSTTAKITTPSRGGTKAKAGEPSAPCSHTVRNGESVGSIAARYR
                     VTRAAVVGANRLDNPHALRTGQRLAIPGCRPPAPTAKDEGPTVLSSEGSVLKRVGPLR
                     VLTELVLVQPDFQEERIALAWPVEGPVISTFGRRARGWHAGIDITADMGSQILAAAPG
                     TVLYSGWIRAYGQIVKIQHSNGFITLYAHNLKNLVEEGEEVEAGQVIATVGRSGHASG
                     PHLHFEVRRHGKAYNPLHVLEPSDQSPIFDDDVAASSSDLDSHE"
     gene            9975..>10211
                     /locus_tag="VNU03_16020"
     CDS             9975..>10211
                     /locus_tag="VNU03_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HWP76029.1"
                     /translation="MAPDTGTDELEGGDEPLETLLAIDPAELAEKPEAREAGDRSREL
                     IALYLQEISRVRLLTAEEEQDLARRVQAGDTDAER"
BASE COUNT         1486 a         3583 c         3754 g         1388 t
ORIGIN      
        1 cccgatcgag gtggtcagcg cggattcgcg ccaggtctac cgcgggctcg acatcggcac
       61 cggcaagccc tccgcggagg agcgggcggc ggtgccgcac cacctcctcg acgtggccga
      121 tccgggcgag cgctacaacg ccgcgcggtt ccggaccgac gcgcgggctg ccatcgcggc
      181 catccacgga cgcgggcggc tgccggtggt ggtgggcggc accggcctct acgtccgcgc
      241 cctgctcaag ggcctcaccc cggcacctcc cgccgatccc gccctgcgcg cggagctgga
      301 ggcgatggcc caggcgcagg gcagcgcggc cctccacgcg cggctggccg cgctggcgcc
      361 cgagatcgcg cgccgcctgc accccaacga ccgggtgcgc gtcgtccgcg cgatcgagat
      421 ccacgccggg agcgcgaccg cccccgcgga gggcgcggag ggagactgga gcggcgccac
      481 gcgggcctgg cggctgctga tgatcggcct cggacgggag cgcgccgcgc ttaacgcggc
      541 gatcgcgctc cgggcgcgcg cgatgctcgc ccgtggcatg atgagcgagg tggagcgcct
      601 gctcgccacg ggccacgatc cggagcggcc accgatggac ggcatcggct accggcctct
      661 ggccctggcc gcgcggggcc ggctgccggt cgaggaggcg ctccggctca tgatccgcga
      721 caccacgcgg tacgcgaagc ggcagatgac gtggttcgcg cgcgatccgg agatccgctg
      781 gatcgacgtg gatgcggcgg gcggcgtcga gggcgccgcc gagacggtgt cgaaacatct
      841 cacgcaggag gggctcaccg agtgaagtcg cgcgagaagg cggtgctggc ggcggtccgg
      901 ctgccccagc agccgcgggt ggtggtggaa gagagcctcg acgagctggg ccgcctggcc
      961 gagtcggccg gggccctcgt ggtggggcgg gcgacccagg accggcgggc gccgaccccg
     1021 ggcctctact tcggaaaggg caaggtggag gagatcaaga cgctctcgaa gcgcgagggc
     1081 gccaacctca tgatctccga cgatcccctc tcgccgatcc aggagcgcaa cctgggcggc
     1141 tcgctcgggg tgcgggtgat cgaccgcacc gcgctcatcc tcgacatctt cgcccagcgc
     1201 gcgcgcacca tggagggcaa gctgcaggtg gagctggccc agctctccta cctgctcccg
     1261 cgcctggtgg ggcagtggaa gcacctcgag cgcaccggcg gcggcatcgg gacgcgggga
     1321 ccgggcgaga tgcagatcga gtccgaccgg cgcatgatcc gccatcgcat ccagaagatc
     1381 cgcgacgagc tgcagcgggt gcgggtgcac cggcgcctgc tccgcgaccg ccgcaaggcg
     1441 agcggggtcc cggtcgtcgc cctcgtcggc tacaccaacg cgggcaagac cacgctgctc
     1501 aaccacctga ccggcgcaga ccgggtcgcg gccgacgcgc tcttcgtcac cctcgatccg
     1561 acggcccgcc tggtcacgag ctcgacccac gcctcgttca tcctgaccga taccgtcggc
     1621 ttcatccgca agctgccgca ccagctggtg gccgcgttca aggccaccct cgaggagctg
     1681 accgaggcgg acgtgctgat ccacgtcgtc gacgcgagcc acccggggct ggacgaccag
     1741 gtgcaggcgg tggaggcgct gctgggcgag ctcgagctga tgggccggcc catgatcgtc
     1801 gcgctgaaca agatcgaccg cctcgagtcg ggggcggccc ggtcgctgct ctcccgcttc
     1861 gacggggtcg cgatctcggc ccgcaccggg gagggcatgg aggcgctccg agagcgggtc
     1921 gacctggcgc tcagcccgcg cgtggcccgg ctgaccctgc gcatccccta cgcggatggg
     1981 ccggcgctcg cggcctgcta cgagcgggga cgggtgctca cgcgccggga cgagcccgag
     2041 ggcatccggg tcgaggtgga gatgccgcgg cggctgatgg cctcgttcga ggggtaccgg
     2101 ctgccaggct gaagtcggtg ctacaatcgg gcctcggccc tcgcggaccg ttccatggga
     2161 ctccccaact ggctcacgac gctgcgcatc ctgctgatcc cggtcttcgt caccctgctc
     2221 gtgtaccggc gggcgggcgc ggcgctcatg gtcttctgcc tcgcgagcct gaccgacctg
     2281 ctcgacggct acgtggcgcg cgtccagggc cgccagacac ggctcggcgc cttcctcgat
     2341 ccggtcgcgg acaagctcct gctcacctcc ggcttcgtca cgctcacctg gctcaaggtc
     2401 ctcccgttct ggatcaccgc ggtggtggtg agccgtgacg tggtgctgag catcggcgtg
     2461 ctggtgatcc acgtggcggg cggcaccgtg cacccctcgc cgacctgggt cggcaagatg
     2521 tccaccgtgt tccagatggc gctcgtgctc gccgccatgg ctggggcgta cttccagctc
     2581 gtcccaccgt tcgtgaccaa gctcctggcc tgggtcaccg ccggcttcac gatcacctcc
     2641 gggctgcagt acatcgtgat gggcctgaag cagctcgaga cgcccacctc ggtgggcggg
     2701 cggctctgac cgtgccccgc cggaccgcgg cgaccgcgga gggcgtggtg gtggcggcgg
     2761 tggcgccgcg gaccgccgcc gcgcgggcgg gcctgcgccc gggcgaccgc atcgtcgcga
     2821 tcaacggcgc gccgctgcgc gacgtgatcg acttccactt ccacgcggga gaggagacac
     2881 tcgacctcgc cctcgaccgg gaggggcgag agcggaccgt acggatgacc cgacggggag
     2941 ccgcgctcgg cctcgagctc gcgcccccgc ggcccgccga gatcgccacc tgcgccaaca
     3001 agtgcgtctt ctgcttcatc caccagctcc cgaagggcat gcgcaagagc ctctacgtga
     3061 aggacgacga cttccggctg tcgttcctgc acggcaacta catcacactg accgatctcg
     3121 acgaggcgga gctccagcgg atcgagaccc agcggctctc ccccctctac gtctcggtgc
     3181 acgccaccga tcccgatctg cgccacgccc tcctcggcca gccgcggctc cgtcgcgagc
     3241 tgctgccggt gatggagcgg ctcgccaagg ccgggatcgc gatgcacgcc cagatcgtgc
     3301 tgtgcccggg atggaacgac ggcgcgcacc tcgagcgcac cgtgcacgag ctggcgcgcc
     3361 tgcatcccgc ggtgcccacc accgcggtgg tgccggtcgg cctcacgcgc caccgtgagc
     3421 ggctgcccga tctccgggcg gtcacgcccg gggaagcggc cacgctggtg cgcgacatcg
     3481 agggctggca gcgcgcgttc caggccacgc tcggcacgcg cttcgtgtgg gccgccgacg
     3541 agctctacct gggcgcgggc ctgcccctgc cgcccgcgcg cgcctacgag ggcttcgcgg
     3601 tcgcggagga cggcatcggc ctcgtgcggc gcttcgagga cgcgtgggcg cgcgcgctcg
     3661 cccggccgcc cgcgccgctc acgcggctgc gccgcgcgac ggtggtgacc ggcgagttgt
     3721 tcgccccgcg catgcgggcg ctcctcgatc ggctcgcggt ccccggcctg acggtggacc
     3781 tggtcccgat ccgcaacgag ttcttcggcc gcggcatcgg ggtggccggc ctgctcaccg
     3841 gccgcgacat ccagctccag ctcggagagg ccggctgcgg cggccgcggg ctcggcgacg
     3901 aagtgctggt gccggcggtg accctccgcg acggggcggg cgtcttcctg gacgacctga
     3961 cccccgccga tctcggcgcc gcgctcggga ccccggtcac cccggtcgag cccaccgccc
     4021 cggccttcct ggccgccctc gtcgggcggt agccggcgag ggcaggaggg gaccagccgg
     4081 tgctgcgccc gatcatcgcc atcgtgggac gccccaacgt gggcaagtcc acgctcttca
     4141 accggctcat cggccgccgc cgctcgctgg tgcgcgacgt gcccggtgtc acacgcgacc
     4201 gcctctacgg caccgcggtc cacgagcgct ggcaggccac cgtggtcgac accggcggct
     4261 tcgacccgag cagcgagtcc gacctggtgc agggcgtgcg gcgccaggtg ctgcaggcgg
     4321 tggacgaggc ggatctactg gtcttcgtcg tggacggtcg cgcgggggtg agcgggctcg
     4381 acgaggagat cgcgggcatc ctccgcaaga gcaaccggcc ggtgctgctc gcggtcaaca
     4441 aggtcgacgg ggccgagcag gagtcaccca ccggcgacgc gtaccggctg ggcttcacac
     4501 cggtgctgac catctccgcc gagcacgggc gcggggtggc cgagctgctc gaggcctgcc
     4561 gcgatctcgc ccccgcggtg ctgccggaga cggggaccga gggcacgcgg gtggccatca
     4621 tcggccggcc caacgtgggc aagtcgtcgc tggtgaacgc ggtgctgggg cacgagcgcg
     4681 tgctcgtgca cgaccagccc ggcaccacgc gggacgcggt ggacaccccg ctgagcttcc
     4741 ggggccgcga ctacctgctg atcgacaccg cgggcatccg gcgcaagggg aaggtgacgg
     4801 agccgctcga gaagctcgcg gtggtgatgg ccctcaagag cctcgagcgc tgccaggtcg
     4861 cggtgctggt ggtggacgcc tccgagggcg tcgccgcgca ggacgcgcac atcgcgggct
     4921 acgccaacga cgcgggccgc gccaccgtgg tcgcggtcaa caaatgggat ctcgttccgc
     4981 ccggcctcgt caccaaaggg gacgtcacca cccagatcta cgagcggctg ccgttcctcg
     5041 agcacgcccc gatctgcttc acgtccgcgg tcgtcgggtt cggtctacgc gatctcttcg
     5101 accaggtcga tctggtggcg gcggaagcgc agaagcgcct gcagcccggc gaggtgctgt
     5161 ccacgctgcg ccaggccgtc gagcggcgcc cgatgtcctc gcgcggcgtg ccgctgcgcg
     5221 tgcaatcggc gcagcaggtg gcgatcggcc cgcccacgtt cgcgctccgc gtgaacctgc
     5281 cggaccagat ccacttctcg tacgagcggt atctgatcaa ctccctgcgc cattcccaca
     5341 gcttcgccgg ctcgccgatc cgtctgctct tccgcaaggc ggtcgggcgg ggcgccaagc
     5401 ggacccggcg agcgccccgc cgggcctgag ccggtatggg ctaactaggc agaaccccta
     5461 gcgatttcgt ttgagcggct caggcactgg tgctataaca atccctagct tttcacgacc
     5521 aggcagccaa acgaggccag caaggcctac gaggccgacg aggatcgagc acgcgtgagc
     5581 cctgagcgcc ccgaggacga cgatttcctg gaggacgaag agcaggagga gtatggacag
     5641 cgcagcatct tcgcgaccgg ttggttccgc gcggtcctgg tcttgctcgt cctcgcggtg
     5701 atcgcggtga tcgcggtgcc gctgatcggc ggctggttcg agccgacgcc accgcccaag
     5761 gtgacgacga cccggccggt cgagcccacg ccggcgccgg cgacgccggc gcccacgccg
     5821 ccgcccgtca ccccggctcc ggtgacgccg ccgcccgtgg cccagctgcc gacgccggcg
     5881 cccacggcgc cgccggccac cccggctccg gtgacgccgc cgcccgcggt gagcaccccg
     5941 cccgcgcggc ccgagatgcc ggccgcctcg acgcccagcc gggcggccga gaaggcgaag
     6001 gcacccgagc gcaccgcgtc cgcgagcagc ggcaagagcg cgagcaaggc ccccgccgcg
     6061 aacggcagct actgggtgca ggtgggcgcg ttcaaggaga gcaagaacgc cgaggggctc
     6121 gcccgcacgc tccgctccga gggattcacg gtgcaggtgg ccaccgtgac gcggggcgag
     6181 ccgttgcacg tggtgcgcgt cggcggctac gccgatcgcg gcaaggccac cgcggcccgc
     6241 gaggagctgc agggcaaggg acattcgggt ttcgtcatgc agggtccggc caagtaaatc
     6301 gacgagagga ggtgacggcg ctgaccaaga acgatctgat caacgcggtg gcggcccacg
     6361 ggctgtcgaa gcggcagtct gcttcggtgg tggagtcgct gttcgacatc atcttccgct
     6421 gcttcgagaa gggtgaggac gtgaagatcg tcggcttcgg gcattttcgg atccgtcaga
     6481 aggcgtcgcg ccgcggccgc aatccccaga ccggcgacag catcgagatc accgcgcgca
     6541 aggtgctgac gttcaagccg agcaagggcc tgaagctccg gatcaacgtc tagaccggag
     6601 gtccgcggga aatgagcacg gttcccgaca agctctacta ccggatcggg gaagtcgaga
     6661 cgatcacgga gatccccgcg tacgtccttc gctactggga gtccgagttc aagctgctcc
     6721 gcccgaagaa gaacccggcg gggcagcgcc tgtaccgcaa ggcggacctc gagctggtcc
     6781 tgcgcatcaa gtcgctgctc tacgaggagc gcctgaccct cgagggcgcc aagaagcgcc
     6841 tgcgcagcga gtcccgcggc ctcgaccaga tggacctggg gctgcgcgga gccaccctgg
     6901 aggacgcgct caagcgcacc cggagccgcc tgcaggccat ccgccagcgc ctcgccgctc
     6961 cgcgatccgg ccgctcgtct tgagtttgcc ccaccgtgcc cgtacaatgc ccgttgggtc
     7021 ggggcgtggc gcagcccggt agcgcacttg actgggggtc aaggggtcgc tggttcaaat
     7081 ccagtcgccc cgaccaattt caatcgacat ggcgcccgtg atcctcgtca ccaacgatga
     7141 cgggattcac gcgcgcggtc tggcggtgct cgccgaggcg ctggccgaga tcggtgaagt
     7201 gtacgtggtg gcgccggatc gcgagcagag cgcggtaggg cacgcgctca cgctgcaccg
     7261 accgctccgg gtcgatcggg tcgcggagcg gaggtacgcg gtgaacggta ccccgtcgga
     7321 ctgcgtgaac ctcgcggtcc tcgggctcct gccggagccg ccggtgctgg tggcgtcggg
     7381 catcaaccac ggcacgaacc tcggcgacga cgtgacgtat tcggggacgg tgtcggcggc
     7441 gatggagggc acgctgctcg gagtgccgtc catggcggtg tcgcaggcgg accccgaggc
     7501 ggagggcggc ttcgaggcgg cgggcgcggt ggcgcgggcg gtggccatgc ggctcctggt
     7561 ggaggggctg ccggccaaga ccctgctgaa cgtgaacgtg ccgcggggag agatcaaggg
     7621 catccggctc acgcggctcg ggcatcgggt ctaccgggag aaggtgatcc gggaggtgga
     7681 cccgcgcggg cggccgtact actggatcgg ggcgggcccg ccggagtggg cggaggacgc
     7741 cagcgccgat atagccgcga tgcacgaggg atgggcgtcg gtgacgccgc tgcacctgga
     7801 cctgacgcat cacggcgcgc tcggccggat gaccgagtgg gaaggcgcgc tgaacgcggt
     7861 actgaagaaa aagaagaagc ggagccggtg acgggcggcg cggagacgaa ggcgggctcg
     7921 gcgcggttcg cgcgcgagcg cgagcggatg gtggaggagc aggtgatcgc gcgcggggtc
     7981 agagacccgc gggtgatcga ggtgatgcga cggctgcccc gccacgtgtt cgtggacgag
     8041 gcgctccgcg atcgggccta cggggaccac ccgctgccga tcggggaggg gcagacgatc
     8101 tcgcagccct tcatcgtggg tcgcatgacc gagctgttgc ggctcggcgg gcgagagaag
     8161 gtactggagg tgggcaccgg ctgcgggtac caggccgcgg tgctcgcgga gctggccggg
     8221 cgcgtgtgta cgatggagcg catcccgcgg ctcgcgagcc gggcacggga gacgctggaa
     8281 gggctcggct accgcaacgt gtgggtgcgg gccgccaacg gcacgctggg atggccggac
     8341 gaggcaccgt tcgaccgcat cctggtggcg gcgggcgggc ccggcgtgcc gccgcccttg
     8401 ttcgagcagc tcgcggaggg cggccgcatg gtgataccgg taggtgaggt cgacggccag
     8461 gtgctgcagg tgatcgagaa ggtgcagggg acgatgcgcg tcaccccgga ttcggggtgc
     8521 gtgttcgtga agctggtggg caagtacgcc tgggagtcgt gagggcccgg gcctgacggt
     8581 tacggacggg gcgtagcgca gcttggtagc gcgcctgctt cgggagcagg aggtcgctgg
     8641 ttcaaatcca gtcgccccga ccatttttcg agtcgtcgtt ttctcgagtc gtcgccggag
     8701 ccggcatggg catcacggga ctgcgcagcg ccgccgagat cgtggtggag gggcgcgtgc
     8761 agggggtcgg gtaccgtgac tacgtccagc gcaaggccgc ctcgctgggg ctggccggct
     8821 atgtcatgaa cctgaagaac ggcaaggttc gcgtgcgggt cgaaggcggg cgcgacctca
     8881 tcgaggagct gatgcacgac ctggagaagg ggccgccgtt gtcgcgcgtg gaccacctga
     8941 ccgtgacgtg gcgcccgctc accggccgct acacggggtt ctccatccgg tacgccgagt
     9001 tcgatccgtg agacacggcc ccgcgcgggc ccgacgggcg atcggccgcg cgttgctcct
     9061 gcccatcctg ctcgtcccca ccgccctcgc ggtcgcttcc gtgtcccacg cccagaccaa
     9121 aaaagccatc gacgccacgc ccacgcccgc gaagacctcg ccccgggagc ccacctccac
     9181 ccggagcacg acggccaaga tcacgacccc gtcccggggc gggaccaagg ccaaggccgg
     9241 cgagccgtcc gctccgtgct cccacaccgt gcggaacggg gagtcggtcg gttccatcgc
     9301 cgcgcgctac cgcgtcaccc gcgcggcggt ggtgggggcc aaccgcctcg acaacccgca
     9361 cgcgctccgg accggccagc gcctcgccat cccgggctgc cgcccgccgg cgccgaccgc
     9421 caaggacgaa gggccgacgg tcctctcgtc cgaaggcagc gtgctgaagc gagtggggcc
     9481 gctgcgggtg ctgaccgagc tggtgctggt gcagccggac ttccaggagg agcgcatcgc
     9541 gctcgcctgg ccggtcgagg ggcccgtcat ctccaccttc ggccggcggg cgcggggctg
     9601 gcatgcgggc atcgacatca ccgccgacat gggctcgcag atcctggccg ccgcccccgg
     9661 gaccgtcctt tacagcggat ggatccgcgc ctatggccag atcgtgaaga tccagcattc
     9721 aaacggtttc atcacgcttt acgcccacaa tctaaagaat ctggtggaag aaggggaaga
     9781 ggtcgaggcc ggccaggtca tcgccacggt gggccgcagc gggcatgcct ccgggcccca
     9841 cctgcacttc gaggtgcgcc gccacggcaa ggcctacaac ccactccacg tgctcgagcc
     9901 ttccgatcag tcgccgatct tcgacgacga cgtggccgcc tccagctcgg acctcgactc
     9961 gcacgagtga tccggtggct cccgatacgg gcaccgatga gctggagggc ggggacgagc
    10021 ccctggagac gctcctcgcg atcgaccccg cggagctggc cgagaagccg gaggcccgcg
    10081 aggcgggcga ccgcagccgt gagctgatcg cgctctacct gcaggagatc tcgcgggtca
    10141 ggctgctgac cgcggaggag gagcaggacc tggcccggcg cgtccaggcc ggcgacacgg
    10201 acgcggagcg g
//
LOCUS       DATMCV010000701         2669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_973549, whole genome shotgun sequence.
ACCESSION   DATMCV010000701 DATMCV010000000
VERSION     DATMCV010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973549"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VNU03_16025"
     CDS             <1..273
                     /locus_tag="VNU03_16025"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HWP76030.1"
                     /translation="VVELKEADWCCGSAGTYNLTQPEMATRLQERKVAHVQATGADAV
                     VTANPGCIIQIAQGLAAKGSPIQVLHIVELLDQAYSPSPPKGERAG"
     gene            287..595
                     /locus_tag="VNU03_16030"
     CDS             287..595
                     /locus_tag="VNU03_16030"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWP76031.1"
                     /translation="MARVKVLDAADLIPGKGALVTVNGQDVALFRRGDEMLAIGNDCP
                     HQGGSLCAGWVEGDIVICPVHGWEFDMRSGACMTVPGESVPRYTATVEDGAIYVEETE
                     "
     gene            592..1119
                     /locus_tag="VNU03_16035"
     CDS             592..1119
                     /locus_tag="VNU03_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HWP76032.1"
                     /translation="MSSPERADRVPLEHKATAPRVIGCFVLTISDSKTPETDTGGALI
                     REHLVAAGHTVVGHAIVRDEPTQVTAVIRKGCADPAVEAFILTGGTGVTSRDSTFEAV
                     EALLDKRLTGFGELFRMLSYAEVGPAAMLSRAQGGVVQGRALFSLPGSPNACRLALEK
                     LILPELGHVLREVRR"
     gene            1198..2358
                     /locus_tag="VNU03_16040"
     CDS             1198..2358
                     /locus_tag="VNU03_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWP76033.1"
                     /translation="MRRPALPLALVLSLVVASTAVAPARAADPLWRDSPGVAAPPDIV
                     RLNDFMHDLSERMKPPLVQVRVRRAAEPQAEGQEQQGTPEERRSAGSGFIIREDGYLV
                     TNAHVVGDAERIQVRLSDGRRFDGRLVGLDERVDLALLKIEASGLPVALLGDSNRARV
                     GEFVLALGHPFGLEQTVSFGIVSRKGAPIQVAAPGFEFIQTDAAVNPGNSGGPLVNMA
                     GEVVGINSMAAVNGSIGFAIPVNLVKALLPQLAEKGKVEWGWLGVSIAEVPDEDAAKF
                     GLKEPKGVLIRQVVVGQPADQGGIRANDVVVSVDGASVEGPRDLQRIISSTPVGKIVK
                     ISLMREGKEQEVAVTVGAYQAAPLRAPRRPGPVPAPRQPQPQPSPPSPQQPR"
     gene            2364..>2669
                     /locus_tag="VNU03_16045"
     CDS             2364..>2669
                     /locus_tag="VNU03_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76034.1"
                     /translation="MPRPARRPAAPFILGLSLAAAVAWAPAAPATAAQVESLLPSDRL
                     TVPDPGQATGRRMTLPLPDCGLDAAGCDEVRLLNELDGWSVNPRIAIRFSGPVALDSV
                     "
BASE COUNT          365 a          975 c          957 g          372 t
ORIGIN      
        1 gtcgtggagc tcaaggaggc cgactggtgc tgcggctcgg ccggcacgta caacctgacc
       61 cagcccgaga tggccacccg cctgcaggag cgcaaggtcg ctcacgtgca ggccaccggc
      121 gcggacgcgg tggtgaccgc caatccgggc tgcatcatcc agatcgcgca gggactcgcc
      181 gcgaagggct cgcccatcca ggtgctccac atcgtggagc tgctcgacca ggcctactcg
      241 ccctcgcccc cgaaggggga gagggcaggg tgagggggcc tctcccatgg cccgcgtgaa
      301 ggtgctggat gccgccgacc tcatccccgg caagggcgcg ctcgtcaccg tcaacggcca
      361 ggatgtcgcg ctcttccgcc gcggcgacga gatgctggcc atcggcaacg actgcccgca
      421 ccagggcggg agcctctgcg ccggctgggt ggagggcgac atcgtgatct gcccggtcca
      481 cggctgggag ttcgacatgc gctccggcgc ctgcatgacc gtgccgggcg agagcgtccc
      541 gcgctacacc gccaccgtcg aggacggcgc gatctacgtc gaggagacgg aatgagcagt
      601 ccggagcgcg ccgaccgcgt gcctctcgag cacaaggcca ccgcgccgcg cgtgatcggc
      661 tgcttcgtgc tgaccatctc cgactcgaag acgcccgaga ccgacaccgg cggcgcgctc
      721 attcgcgagc acctcgtcgc ggcagggcac accgtggtcg gccacgcgat cgtgcgcgat
      781 gagccgacgc aggtgaccgc ggtgatccgg aagggctgcg cggatccggc cgtcgaggcc
      841 ttcatcctca ccggtggcac cggggtcacc tcgcgcgact cgacgttcga ggcggtggag
      901 gcgctgctgg acaagcggct caccggcttc ggcgagctgt tccgcatgct ctcctacgcg
      961 gaggtaggcc cggccgccat gctctcgcgc gcgcagggcg gcgtggtgca gggccgggcg
     1021 ctcttctcgc tgcccggctc gcccaatgcc tgccggctcg cgctggagaa gctgatcctg
     1081 cccgagctgg ggcacgtgct gcgcgaggtc cggcgctgat cgctccgggt cgccgctaaa
     1141 ccccatcgcg gtataatcga cccgtccccg agcaccacac ccccaggagg cttgcagatg
     1201 cgtcgtccgg ctcttcccct cgcgctcgtc ctgtcgctcg tcgtggcctc gacggccgtc
     1261 gccccggccc gagccgcgga tccgctgtgg cgggacagcc cgggagtcgc cgcgcccccg
     1321 gacatcgtcc ggctcaacga cttcatgcac gacctctcgg agcgcatgaa gccgccgctc
     1381 gtccaggtgc gggtgcggcg ggccgcggag ccgcaggccg aggggcagga gcagcaaggg
     1441 acgcccgagg agcgccgcag cgcgggctcg ggcttcatca tccgcgagga cggctacctg
     1501 gtgacgaacg cgcacgtggt gggcgacgcc gagcgcatcc aggtccggct ctcggacggc
     1561 cgccgcttcg acggccggct ggtcgggctc gacgagcgcg tggacctggc cctgctcaag
     1621 atcgaggcaa gcggcctgcc ggtcgcgctc ctcggcgact ccaatcgcgc gcgggtgggc
     1681 gagttcgtgc tggcgctcgg ccatcccttc gggctcgagc agaccgtgtc cttcggcatc
     1741 gtgagccgca agggcgcccc catccaggtg gcggcccccg gcttcgagtt catccagacc
     1801 gacgcggcgg tgaaccccgg caactcgggc gggccgctcg tcaacatggc gggtgaggtc
     1861 gtcgggatca acagcatggc cgcggtcaac ggctccatcg gcttcgcgat cccggtgaac
     1921 ctggtgaagg cgctgctgcc ccagcttgcc gagaagggca aggtcgagtg gggctggctc
     1981 ggcgtcagca tcgcggaggt gcccgacgag gacgccgcca agttcgggct caaggagccg
     2041 aagggcgtgc tgatccgtca ggtggtggtc ggacagcccg ccgatcaggg tgggatcagg
     2101 gccaacgacg tggtggtctc ggtggacggc gccagcgtgg aagggccgcg cgatctgcag
     2161 cggatcatct cgagcacgcc ggtgggcaag atcgtgaaga tctcgctcat gcgcgagggc
     2221 aaggagcagg aggtcgcggt caccgtgggt gcctaccagg ccgccccgct gcgtgcgcct
     2281 cgccgccccg ggcccgtgcc ggcgccgcgg cagccccaac cgcagccgtc gcctccgtcg
     2341 ccccagcagc ctcgctgagg ccggtgcctc gcccggcccg ccgcccggcc gcccccttca
     2401 tcctcgggct gtcgctggcg gcggcggtcg cctgggcgcc ggcggccccg gcgaccgcgg
     2461 cccaggtcga gtctctcctt ccctctgacc ggctgaccgt tccggatccc ggccaggcca
     2521 ccggccgccg catgaccctg ccgctacccg actgcggcct cgacgccgcc ggctgcgacg
     2581 aggtgcgcct cctgaacgag ctcgacggct ggagcgtcaa tccgcggatc gccatccgct
     2641 tctccgggcc agtggcgctc gacagcgtc
//
LOCUS       DATMCV010000702         4911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_236034, whole genome shotgun sequence.
ACCESSION   DATMCV010000702 DATMCV010000000
VERSION     DATMCV010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236034"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VNU03_16050"
     CDS             <1..290
                     /locus_tag="VNU03_16050"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76035.1"
                     /translation="PERVALIASGGMSHFPGTDRYASPEFEYDRGLLEVLADGRGREL
                     GRLTGEELDKRGNIELRTWITLLGAVGDARARVYCYEPSWHHGNAVVEWPL"
     gene            298..576
                     /locus_tag="VNU03_16055"
     CDS             298..576
                     /locus_tag="VNU03_16055"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76036.1"
                     /translation="MSETAHYRFPPSSAYALNRCLYALKSDREFAERFVADPAAVTGA
                     LGLTEGERADLRRMDRDALVARGAHPYLVFMAALRLKMLAEPGTLEYF"
     gene            complement(587..964)
                     /locus_tag="VNU03_16060"
     CDS             complement(587..964)
                     /locus_tag="VNU03_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76037.1"
                     /translation="MTRYITFVAVAGLVALQGGAVVASGAAQSAHPLQPTGRIARLDR
                     GAGMIEGKLTRVDGRTETVDVSIGLLGLLGKTIEVNHDTLIQVNGREARFADLQEGAK
                     VKAFYEERGAKLVGTRLEVTTGS"
     gene            1147..1320
                     /locus_tag="VNU03_16065"
     CDS             1147..1320
                     /locus_tag="VNU03_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76038.1"
                     /translation="MTRRFRRLARRSRAVAFAWAVLRSYREGRRRGQSPLRALRFGRA
                     VARWRRQNGAAHS"
     gene            1349..1804
                     /locus_tag="VNU03_16070"
     CDS             1349..1804
                     /locus_tag="VNU03_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76039.1"
                     /translation="MPALVLALILTAGARSPLEAQAPAAPPGTPAPAATVSELQQTLA
                     AASGRFQAMDTAGVLAYVSDRYRNGPITKSSVRDNLLAMFALYDTVRAQVRLDEVRVV
                     NGAAWVYSTGEISGRLRGLGVWTTVLSWEREPEVARREGAAWRLEGPGP"
     gene            1945..4305
                     /locus_tag="VNU03_16075"
     CDS             1945..4305
                     /locus_tag="VNU03_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76040.1"
                     /translation="MLRAAARGGHWGIFRLTAVLLLYALTLFTGATLLFVMQPMVGKM
                     ILPLLGGTPAVWSTCMVFFQAALLGGYAYAHALTARVRPSRQVLVHLIALALPFAVLP
                     LAVSPRLLRGGEANPVLDVLTILSLSVGLPFLVVSATAPLLQRWFTHTGHPAARDPYF
                     LYAASNLGSMLALLGYPTLVEPHLHLQGSTWLTQTALWSLGYATLAVLIALCALTLWW
                     KPAPAPAPAIAEAESSPAPAERRPRWPRRIHWVVLAFVPSSLLLGVTTYITTDLAAVP
                     LLWVLPLAIYLLTFILAFGRWPRLLHHVVVALALPLSLLVIFLMVSALPERIWVIVLW
                     HFGLLFVIALACHGALALDRPGPLHLTEFYLLISVGGVLGGLFNALVAPLVFSSLIEY
                     PLMMVLACVLLAAPSSPGLGLGAGARRALLAVAATILLTVVLYSESATLRVDFAFLAR
                     VLDLGSGRVSEWLDPAERLLNKLLIYGPPLVIAWFLRRRPLHLAVTLAAVLLISGFVD
                     ARNNDQIRQSRSFFGVLRISRDRDPKGYTELRHGTTLHGRQSLEPSRRAEPLSYYQRK
                     GPIGQLFAELDRRAGSVHMAVVGLGTGTLAAYARPGDRVTFYEIDRMVRDIAFDPAYF
                     TYATDARDRGVTMRLELGDARIRMEAVKRDRPEERYDVILVDAFSSDAIPVHLLTREA
                     LRLYFDLLGPRGILALHISNRYLRLEPVVAALAEDGRYARMVEHGDTGDIRGGVEASW
                     AILARRPEDFGEMASDPRWAAAHLDVEPAVSAWTDDFHNLLSVFKW"
     gene            complement(4295..>4911)
                     /locus_tag="VNU03_16080"
     CDS             complement(4295..>4911)
                     /locus_tag="VNU03_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP76041.1"
                     /translation="LKINVALAELPDFIACPGTTPGPQHRGTIHVCPDLDYIERAFDD
                     AKYGRPSARPVLECTIPSVVDPTVAPPGRHLMSIFVQYAPYTLRDGSWEEMREPFADR
                     CLALLDEYAPNFSRSVLARQVLAPPDLERVFGLTGGNIFQGAMTPGQLFSLRPVPGHA
                     GYRTPIGGLYLCGAAAHPGGGVMGTPGYNAAREILRARRPRVTT"
BASE COUNT          612 a         1834 c         1702 g          763 t
ORIGIN      
        1 gcccggagcg cgtcgcgctc atcgcctcgg gcggcatgtc ccacttcccg ggaaccgacc
       61 gctacgcctc ccccgagttc gagtacgacc gcggtttgct cgaggtgctg gccgacggcc
      121 gtgggcggga gctcggccgg ctgaccggcg aggagctcga caagcggggc aacatcgagc
      181 tgcggacctg gatcaccctg ctcggcgcgg tgggcgacgc gcgggcacgg gtgtactgct
      241 acgagccctc gtggcaccac ggcaatgcgg tggtggagtg gccgctctga gcccgcgatg
      301 agcgagaccg cccattaccg cttcccgccg tcgagcgcct acgcactgaa ccggtgcctc
      361 tacgcgctca agtcggaccg ggagttcgcg gagcgcttcg tcgccgaccc ggcggcggtc
      421 accggcgcgc tgggactgac cgagggcgag cgggccgacc tgcgccgcat ggatcgcgac
      481 gccctggtgg cccgcggcgc ccatccctac ctcgtgttca tggccgcgct ccggctcaag
      541 atgctcgccg agcccgggac cctggagtac ttctaggcgt gcacgtttag ctgccggtcg
      601 tcacctcgag gcgagtgccg accagcttgg cgccgcgctc ctcgtagaac gccttcacct
      661 tcgccccctc ctgcaggtcg gcgaaccgcg cctcgcggcc gttcacctgg atgagggtgt
      721 cgtggttgac ctcgatggtc ttgccgagga ggcccagcag gccgatcgag acgtcgaccg
      781 tctcggtgcg gccatccacc cgggtcagct tgccctcgat catcccggca ccgcggtcga
      841 gccgggcgat gcgccccgtc ggctgcagcg ggtgcgccga ctgggccgcg cccgaggcca
      901 cgaccgctcc gccctgcaac gcgacgagcc ccgcaaccgc cacgaaggtg atgtagcgcg
      961 tcatggcccg gccctcctct gcattttttc ctcggggcaa ttggccccgc tcccgcatat
     1021 aggagagcaa gggcgatacc agacgaggta aaggccgcct ccaggtagcg aatcgcctcc
     1081 ttccggcgtg ggaggatgta acccttacac gagcatggga cgtaagttgt tccaggaggg
     1141 gaaccaatga cacgacgctt cagacggctg gcgcggcgct cgcgcgcggt ggccttcgcg
     1201 tgggcggtgc tgcggagcta ccgggagggc cggcgtcggg gccaatcccc gctgcgcgcg
     1261 ctgcgcttcg gtcgcgcggt ggcgcgctgg cgacggcaga acggagccgc gcattcgtag
     1321 ccatcccggg cgtcgcccgg ggcggctgat gcccgccctc gtcctcgccc tgatcctgac
     1381 cgccggcgcc cgctccccgc tggaggccca ggcgcccgcc gcgccgccgg gcacgccggc
     1441 tcccgccgcg acggtgagcg agctgcagca gaccctggcg gccgccagcg gccgcttcca
     1501 ggcgatggac accgcggggg tgctcgccta cgtctccgat cgctatcgca acggccccat
     1561 caccaagtcg agcgtccgcg acaacctcct ggccatgttc gccctctacg acaccgtccg
     1621 ggcgcaggtg cgcctcgacg aggtgcgcgt ggtgaacggg gcggcgtggg tctactccac
     1681 cggggagatc tcgggacgac tgcgcggcct cggcgtgtgg acgacggtgc tctcgtggga
     1741 gcgcgagccg gaggtggcgc ggcgcgaggg cgcggcctgg cggctcgaag gcccggggcc
     1801 ctagaaccgc ggcgaggagt gatccggctg tcgttgcgaa gcgcgaaggt gcccacgccg
     1861 aacccgagga cggggccggg cgagggcggc aaggcgagca tcgcggagtc gattctaccg
     1921 tgactcgcgg acccggatac ggctatgcta cgtgccgccg cccgaggcgg ccactggggg
     1981 atcttccgtc tgaccgccgt cctgctgctc tacgcgctca ccctgttcac gggagccacc
     2041 cttctcttcg tgatgcagcc gatggtgggc aagatgatcc tgccgctcct gggcggcacc
     2101 cccgcggtgt ggagcacctg catggtgttc ttccaggccg cgctcctcgg cgggtacgcc
     2161 tacgcgcacg ccctcaccgc ccgcgtccgg ccctcgcgcc aggtcctcgt ccacctcatc
     2221 gctctcgcgc tgcccttcgc ggtcctgccg ctcgcggtga gcccgcgcct gctgcgcggc
     2281 ggcgaggcca acccggtgct ggacgtcctc acgatcctct cgctgtcggt gggcctgccc
     2341 ttcctggtgg tgagcgccac cgccccgctg ctccagcggt ggttcaccca caccgggcac
     2401 cccgccgcgc gcgatcccta ctttctctac gcggccagca acctgggcag catgctggcc
     2461 ctcctgggct atcccaccct ggtggagccg cacctgcacc tgcaaggatc gacctggctc
     2521 acccagaccg cgctctggag cctgggctac gcgacgctgg ccgttctcat cgcgctctgc
     2581 gcgctgaccc tctggtggaa gcccgcgccc gcccccgcgc ccgctatcgc ggaggcggag
     2641 agctcgccgg ccccggccga gaggcgcccg cggtggccgc ggcgaattca ctgggtcgtg
     2701 ctcgccttcg tgccgtcgag cctgctcctc ggggtcacca cctacatcac caccgacctg
     2761 gccgcggtgc cgctgctctg ggtgctgccg ctcgcgatct acctgctgac cttcatcctc
     2821 gccttcggcc gctggccgcg gctgctccat cacgtggtgg tggcgctcgc gctgcccctc
     2881 agcctgctcg tgatcttcct gatggtctcc gccctgcccg agcgcatctg ggtcatcgtg
     2941 ctctggcact tcggtctgct cttcgtcatc gcgctcgcct gccacggggc gctggcgctc
     3001 gaccggcccg gcccgctcca cctcaccgag ttctacctgc tgatctcggt gggcggcgtg
     3061 ctgggcgggc tcttcaacgc tctggtggcc ccgctcgtct tctcctcgct gatcgagtat
     3121 ccgctgatga tggtgctggc ctgcgtgctc ctcgccgcgc cgagcagccc cgggctcggc
     3181 ctgggcgccg gggcgcggcg cgcactgctc gcggtggcgg ccacgatcct gctcacggtg
     3241 gtgctctact cggagtcggc caccctgcgc gtcgacttcg cgttcctggc ccgggtgctc
     3301 gacctcggca gcgggcgggt gagcgagtgg ctcgatcccg ccgagcgctt gctcaacaag
     3361 ctcctgatct acgggccgcc tctcgttatc gcgtggttcc tgcgccgccg cccgctccac
     3421 ctggccgtga ccctcgccgc ggtgctcctg atctcgggct tcgtggacgc gcgcaacaac
     3481 gaccagatcc gtcagagccg ctccttcttc ggcgtgctgc gcatctcgcg cgaccgcgac
     3541 cccaagggct acaccgagct gcgccacggc accacgctgc acgggcgcca gagcctcgag
     3601 ccctcccggc gcgccgagcc cctgtcctac taccagcgca agggcccgat cggccagctc
     3661 ttcgccgagc tcgaccggcg cgccggcagc gtgcacatgg cggtggtcgg gctcggcacc
     3721 ggcaccctcg cggcctacgc gcggccgggc gatcgcgtca ccttctacga gatcgaccgg
     3781 atggtgcgtg acatcgcctt cgacccggcc tacttcacct acgcgaccga cgcgcgcgac
     3841 cgtggggtga ccatgcggct cgagctgggc gacgcgcgca tccggatgga ggcggtgaag
     3901 cgggaccggc ccgaggagcg ctacgacgtg atcctggtgg acgccttcag ctcggacgcc
     3961 atcccggtcc acctgctcac ccgcgaggcg ctccgcctct acttcgacct gctgggaccg
     4021 cgcggtatcc tcgcgctcca catctcgaac cgctacctgc gcctcgagcc ggtggtggcc
     4081 gcgctggccg aggacgggcg ctacgcgcgc atggtcgagc acggcgacac cggcgacatc
     4141 cgcggcggcg tcgaggcgag ctgggcgatc ctggcccggc ggccggagga cttcggcgag
     4201 atggcgtccg acccccgctg ggccgcggcg cacctcgacg tggagccggc cgtctccgcc
     4261 tggaccgacg acttccacaa cctcctgtcg gtcttcaagt ggtgacgcgc ggccggcgcg
     4321 cgcggaggat ctcccgcgcc gcgttgtagc cgggcgtgcc catgaccccg ccgcccgggt
     4381 gggccgccgc gccgcagagg tagagaccgc cgatcggggt gcggtagccg gcgtggccgg
     4441 gcaccgggcg cagcgagaag agctggcccg gggtcatcgc gccctggaag atgtttcccc
     4501 cggtgagccc gaacacgcgc tccagatcgg gcggggccag cacctgccgc gccagcaccg
     4561 agcggctgaa gttgggcgcg tactcgtcga gcagcgcgag gcagcgatcc gcgaagggct
     4621 cgcgcatctc ctcccagctc ccgtcgcgca gcgtgtaggg cgcgtactgc acgaagatcg
     4681 acatcaggtg ccggcccggc ggggccaccg tcgggtccac caccgacggg atcgtgcact
     4741 cgagcaccgg gcgggccgag ggacggccgt acttggcgtc gtcgaaggcc cgctcgatgt
     4801 agtcgaggtc ggggcagacg tggatggtcc cgcggtgctg cgggcccggc gtggtgcccg
     4861 gacaggcgat gaaatcgggc agctcggcga gcgccacgtt gatcttgagc g
//
LOCUS       DATMCV010000703         6367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_770670, whole genome shotgun sequence.
ACCESSION   DATMCV010000703 DATMCV010000000
VERSION     DATMCV010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770670"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VNU03_16085"
     CDS             <1..470
                     /locus_tag="VNU03_16085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP76042.1"
                     /translation="VTEPLPSLPPVFALIVDLDTGFYVHRQGLSAVLMGGTDKDIKPG
                     LDTEVDWDAFDAVFRAAAVRVPPLAEARVMRAYAGVRDLTPDYHGILGPAAQAPGLHV
                     ACGFSGHGFMHAPAIGILMAEILLDGRASSMDVGALSLDRFAAGKLRAEANMF"
     gene            474..1586
                     /locus_tag="VNU03_16090"
     CDS             474..1586
                     /locus_tag="VNU03_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018849 - muconate cycloisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018850 - chloromuconate cycloisomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconate/chloromuconate family cycloisomerase"
                     /protein_id="HWP76043.1"
                     /translation="MKLADLRVVRADIPVQRPHKMSFTTLETVNFVFVRLETSDGHVG
                     WGEAACLGGPTWSEESAESVAVTILRYIAPWLVGRDPTAIEALRIEMARRVQGNPFAR
                     AAVEMALWDIAGRALGVPVYRLLGGRVRDRVPLSWSLAVADPRAEIEEAQAKIARGHR
                     IFKIKTGAGALADDVERVSRLREALGPAISLRVDANQGWDRPTALRAVRALEPYRLDF
                     VEQPVPRWDLEGLAMIAERAAMPIMADESCFTDHDALELARRGSISILSLKLTKSGGL
                     LGSLAIARVAEAAGMGCYVGCMIETSLGTAAYLHLALAAAPVTWGCELFGPLLLAGDV
                     TRQPVQYADGCILALDGPGWGVDVDETALAEWSRRC"
     gene            1594..2352
                     /locus_tag="VNU03_16095"
     CDS             1594..2352
                     /locus_tag="VNU03_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76044.1"
                     /translation="MSGAAPAAAGPVLETRSVVKRFGGVVAVDGLSLALARGEIRGLI
                     GPNGSGKTTTVNILSGLHRLDAGEIHLDGRRVDRLRPHEIAARGVARTFQIPKLFGNM
                     TVLENVLLPGHAERAHATSRPMAEIQASARRQLEFVAMDRHRHALAKQLSGGQAMLVQ
                     IARALMIHPLQVVLMDEPFAGVHPTIREVIMEAILRMNREHGVTFLIVSHEMAEVRRL
                     CGRVSVMHDGRLIAEGPLAQVANDPVVLEAYLGK"
     gene            2353..3075
                     /locus_tag="VNU03_16100"
     CDS             2353..3075
                     /locus_tag="VNU03_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76045.1"
                     /translation="MALLEVDQVAAGYVEGIDILAGIGLRVEAGSVTGIIGPNGAGKS
                     TLLKTIFGFLHPREGRIVHDGREIQRAAPHEIKRLGVSYVPQGANVFPQLTVQENLLL
                     GAWVERGDRARVGQMLERAYATFPRLREKRRHRATSLSGGEAKMLSLAKELVTDPKLL
                     LVDEPSAGLAPRISTEVYARLVEARATGVTILLVDQNITKAVEVSDYLYVIEGGQVRR
                     QGPQADFADHLRELIRDSLLGA"
     gene            3080..4054
                     /locus_tag="VNU03_16105"
     CDS             3080..4054
                     /locus_tag="VNU03_16105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76046.1"
                     /translation="MAGLLRRLGLCLVTYAALAWVPLVTSEYHTHILVISLYYVILAV
                     GWNLLAGYTGQFSLAHHTFAGIGAYTSALLVQRTGIPILVGVVAGAVVAAAVGYGLGS
                     LCLRMRAIYLALATWAFAESVRLLVTVEYQITRGDLGLAAPLLFGTPRATPYYYLFLG
                     LALVALLVAWQIVHSRIGAYLRAIRDDEEAAATMGVDTFRWKRFAFVVSAVFAAVAGA
                     FQGHYIGLLSPTPMKFNEMATVIIMVITGGLRTFAGPALGAVFIELLSELLRRWGEIR
                     MVLFAVLVIAVVRLYPAGLVGLLRAAGARIGARLPGLAPLLGVGRPTD"
     gene            complement(4071..4961)
                     /locus_tag="VNU03_16110"
     CDS             complement(4071..4961)
                     /locus_tag="VNU03_16110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76047.1"
                     /translation="MDFHLLSQYVLSGLVIGVIYSLMALGITFIYSIMKMINWSMGEF
                     YMIGSYVQYFLLSSVLGWDRWYLALPLAMASVFLLGVVVQRFLLRPMYVGGVERRDEY
                     ATVMTIALMVLFRNLAIVLGGPNQYSPRDYAKPTMLLTLPLSGNRFVALVGALVLLAL
                     FYLLVTRTWVGRALRGAAQNRIGIQTAGIDVLRLDMLAFGVGVAMAAAAGALLAPDFL
                     VYPENGAISTFKGFEIIVIGGLGSILGSVVGGVLLGVIEALGSVFVSPAYKDLYGFLL
                     LIALLALRPNGLFGERERTV"
     gene            complement(5031..6230)
                     /locus_tag="VNU03_16115"
     CDS             complement(5031..6230)
                     /locus_tag="VNU03_16115"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76048.1"
                     /translation="MSRRLTAIVLALLTLAVLAPPALAQKPVKIGVLTPLSPPGDPAA
                     GQLIVRGAKMAADEINAKGILGGRKIELVIEDDSGTPEKGAAGFRKLATQDGVVAVLG
                     QFHSSVALAVQDLAEQFKVPIFATQASARAFTEKHLNYSFRTHVIDTDRVQLWNRWIK
                     DKGFKKVALLAENTDYGVGLVEDTKKLFPSTAGGAELKTIVFDRAVVDLTPQLLEIKS
                     WKPDVVINIGVGTPAYLIIKQAADVGLLPATPMLVSYDMPARAEFWKNLGEKGNYVSF
                     LVYYHPTMKLTPRGEAFRAKYREQFKEDPVYASLNGYAQVVLIADALEAAKSDTSDAL
                     VKSLLANKFQGWNATISFSRGEGPYWQQWTPPMLLMQYTKTDMPFTEAKIIYPTEFKT
                     GELVVPR"
BASE COUNT          906 a         2250 c         2234 g          977 t
ORIGIN      
        1 tggtgaccga gccgctcccg agcctgcccc cggtgttcgc gctcatcgtg gacctcgaca
       61 ccggcttcta cgtccaccgc cagggcttga gcgcggtgct gatgggcggc accgacaagg
      121 acatcaagcc gggcctcgac accgaggtgg actgggacgc cttcgacgcg gtcttccggg
      181 ccgcggcggt ccgggtgccg ccgctcgccg aggcgcgcgt catgcgcgcc tacgcgggcg
      241 tgcgggacct gacccccgac taccacggca tcctcgggcc ggcggcgcag gcgccggggc
      301 tccacgtggc ctgcggcttc tccggccacg gcttcatgca cgcgcccgcc atcggcatcc
      361 tcatggccga gatcctgctc gacggccgcg ccagcagcat ggacgtcggc gccctgtccc
      421 tcgatcgctt cgcggcggga aagctccgcg ccgaagccaa catgttctag gccatgaagc
      481 tcgccgacct ccgcgtggtg cgcgccgaca tcccggtgca gcgcccgcac aagatgtcct
      541 tcaccaccct cgagacggtg aacttcgtct tcgtccgcct cgagacctcc gacggccacg
      601 tgggctgggg agaagccgcc tgcctcggcg gacccacctg gagcgaggag tcggccgagt
      661 cggtggccgt gaccatcttg cgctacatcg ccccgtggct cgtcgggcgt gacccgaccg
      721 cgatcgaggc gctccggatc gagatggccc gacgcgtcca gggcaatccg ttcgcccgtg
      781 ccgcggtcga gatggccctc tgggacatcg cgggccgggc gctgggcgtc ccggtctacc
      841 ggctcctcgg gggacgcgtg cgggatcgcg tgccgctctc ctggtcgctc gcggtggcgg
      901 acccgcgcgc cgagatcgag gaggcgcagg ccaagatcgc gcggggccac cgcatcttca
      961 agatcaagac cggggccggc gccctcgccg acgacgtcga gcgcgtgagc cgcctccgcg
     1021 aggccctggg ccccgcgatc tcgctccggg tggacgccaa tcagggctgg gaccggccga
     1081 ccgcgctccg ggccgtccgg gccctcgagc cctaccgcct cgacttcgtc gagcagcccg
     1141 tgccccgctg ggacctcgag ggtctcgcca tgattgccga gcgagccgcg atgccgatca
     1201 tggccgacga gtcctgcttc accgatcacg atgcgctcga gctggcccgg cgcggctcga
     1261 tctcgatcct gagcctcaag ctgacgaagt cggggggcct gctcggcagc ctcgcgatcg
     1321 cgcgggtcgc ggaggcggcc ggcatgggct gctacgtggg ctgcatgatc gagacctcgc
     1381 tcggcaccgc cgcgtatctc catctggccc tggccgccgc tccggtcacc tggggctgcg
     1441 agctcttcgg gccgctcctg ctcgcgggcg acgtcacccg acagccggtg cagtacgccg
     1501 acggctgcat cctcgcgctg gacggtcccg gctggggcgt ggacgtcgac gagaccgcgc
     1561 tcgccgagtg gtcacgccga tgctagccgc gtggtgagcg gggctgcgcc ggccgccgcg
     1621 gggccggtgc tcgagacccg cagcgtcgtg aagcggttcg gcggcgtggt cgcggtggac
     1681 gggctctcgc tggccctggc ccgcggcgag atccgggggc tgatcggccc caacgggtcg
     1741 ggcaagacca ccaccgtcaa catcctctcg ggcctccacc gcctggacgc gggcgagatc
     1801 catctcgacg gccggcgcgt ggaccgcctg aggccccacg agatcgccgc gcgcggagtg
     1861 gcccgcacct tccagatccc gaagctcttc gggaacatga cggtgctcga gaacgtcctg
     1921 ctcccgggcc acgcggagcg ggcccacgcc acctcgcgcc cgatggcgga gatccaggcg
     1981 agcgcgcgcc gccagctcga attcgtcgcc atggatcggc accgccacgc cctcgccaag
     2041 cagctctcgg gcgggcaagc gatgctggtg cagatcgccc gcgccctgat gatccatccc
     2101 ctccaggtgg tcttgatgga cgagccgttc gcgggtgtgc atccgaccat ccgcgaggtc
     2161 atcatggagg cgatcctccg catgaaccgg gagcacgggg tgaccttcct gatcgtctcc
     2221 cacgagatgg ccgaggtgcg gcggctctgc ggccgggtct cggtcatgca cgacggacgc
     2281 ctcatcgcgg aaggcccgct cgcccaggtg gcgaacgatc ccgtcgtcct cgaagcctac
     2341 ctgggcaagt agatggcgct gctcgaggtg gaccaggtcg cagccggcta cgtcgagggc
     2401 atcgacatcc tggccggcat cggcctgcgc gtcgaggcgg gcagcgtcac cggcatcatc
     2461 ggaccgaacg gggcggggaa gtcgacgctg ctcaagacca tcttcggctt cctccatccc
     2521 cgcgagggcc gcatcgtcca cgacgggcgg gagatccagc gggcggcccc tcacgagatc
     2581 aagcggctcg gggtgagcta cgtgccccag ggcgcgaacg tctttcccca gctcaccgtg
     2641 caggagaacc tgctcctcgg cgcctgggtg gagcgcggcg accgcgcccg cgtcggccag
     2701 atgctggagc gcgcctacgc gacgttcccg cggctccggg agaagcggcg gcaccgcgcc
     2761 acctcgctga gcggcggcga ggccaagatg ctctcgctcg ccaaggagct ggtgaccgac
     2821 ccgaagctgc tcctggtgga cgagccctcc gccgggctcg ccccccgcat ctcgaccgag
     2881 gtgtacgcgc ggctcgtgga ggcgcgcgcc accggcgtca cgatcctcct ggtggaccag
     2941 aacatcacca aggcggtgga ggtctcggac tacctatacg tgatcgaggg gggacaggtc
     3001 cgccggcagg gcccccaggc cgacttcgcg gatcaccttc gcgagctgat ccgcgactcg
     3061 ctcctcgggg cctgagggcg tggcgggcct cctccggcgc ctgggcctct gcctcgtgac
     3121 ctacgcggcg ctggcgtggg tgccgctcgt cacctccgag tatcacaccc atatcctcgt
     3181 catcagcctc tactacgtga tcctcgcggt gggctggaac ctcctcgcgg gctataccgg
     3241 ccagttctcg ctggcccatc acacgttcgc cgggatcggc gcctacacct cggcgctcct
     3301 cgtgcagcgc accgggatcc cgatcctggt cggggtggtg gcgggggcgg tggtggccgc
     3361 cgcggtcggc tacggcctcg gcagcctctg cctgcgcatg cgcgcgatct acctggcgct
     3421 ggccacctgg gccttcgcgg aatcggtccg cctgctggtc acggtggagt accagatcac
     3481 gcgcggggat ctggggctgg ccgcgccgct gctcttcggg acgccgcgcg cgacgcccta
     3541 ctactacctg ttcctgggcc tggccctcgt ggccttgctg gtggcctggc agatcgtcca
     3601 ctcgcggatc ggcgcctacc tgcgggccat ccgcgacgac gaggaggccg ccgcgaccat
     3661 gggggtggac accttcaggt ggaagcgctt cgcgttcgtc gtctccgcgg tcttcgcggc
     3721 ggtggcgggc gccttccagg ggcactacat cggcctgctc tcgccgaccc cgatgaagtt
     3781 caacgagatg gccaccgtca tcatcatggt gatcaccggc ggcctgcgca ccttcgcggg
     3841 gccggcgctg ggcgccgtct tcatcgagct gctctcggag ctgctgcggc gctggggcga
     3901 gatccggatg gtgctgttcg ccgtcctggt gatcgcggtg gtccggctct acccggcggg
     3961 cctggtgggg ctgctgcgcg cggcgggcgc gcgcatcgga gcgcggctgc ccgggctggc
     4021 cccgctcctc ggagtcgggc ggccgaccga ctgaggcggc cgcggtcgga tcagaccgtg
     4081 cgctcgcgct ccccgaagag gccgttgggt cggagcgcca gcagggcgat cagcagcagg
     4141 aagccgtaga ggtccttgta ggcgggcgag acgaacaccg agcccagggc ctcgatcacg
     4201 ccgagcagca ctccgcccac cacgctgccc aggatcgacc cgaggccccc gatgacgatg
     4261 atctcgaagc ccttgaaggt cgagatggcc ccgttctccg gatagaccag gaagtcgggc
     4321 gcgagcagcg cgccggccgc ggcggccatc gccacgccca ccccgaaggc cagcatgtcg
     4381 aggcggagca cgtcgatgcc ggcggtctgg atgccgatcc ggttctgggc cgcgccccgg
     4441 agcgcccgcc ccacccaggt gcgcgtgacc agcaggtaga agagcgcgag cagcaccagc
     4501 gcgccgacga gggccacgaa gcggttgccg gagagcggga gcgtcagcag catcgtgggc
     4561 ttcgcgtagt cgcgcgggga gtactggttg ggcccgccca gcacgatcgc gaggttccgg
     4621 aagagcacca tgagggcgat cgtcatgacc gtcgcgtact cgtcgcgccg ctccaccccg
     4681 cccacgtaca tcggccgcag gaggaatcgc tggaccacga ctccgagcag gaacaccgac
     4741 gccatcgcga ggggaagggc gaggtaccag cggtcccagc ccagcaccga ggagagcagg
     4801 aagtactgca cgtagctgcc gatcatgtag aactcgccca tcgaccagtt gatcatcttc
     4861 atgatcgagt agatgaacgt gatgccgagc gccatgaggc tgtagatgac cccgatgacg
     4921 aggccggaga ggacgtactg ggagaggagg tggaagtcca tcgatcgcgc ggccgcggcg
     4981 ccggggccgg gagcgcgcga cttcggctcc cggcccggcc cgacggtcga ctagcgggga
     5041 acgaccagct cgcccgtctt gaactcggtc ggatagatga tcttggcctc ggtgaacggc
     5101 atgtcggtct tggtgtactg catcagcagc atcgggggcg tccactgctg ccagtacggc
     5161 ccctcgccgc ggctgaagct gatcgtcgcg ttccagccct ggaacttgtt ggccagcagc
     5221 gacttcacca gggcgtcgct cgtgtcgctc ttggccgcct ccagcgcgtc cgcgatgagg
     5281 accacctgcg cgtagccgtt cagcgacgcg tagaccgggt cctccttgaa ctgctcgcgg
     5341 tacttggcgc ggaaggcctc cccgcgcggg gtgagcttca tggtcgggtg gtagtagacc
     5401 aggaacgaga cgtagttgcc cttctcgccc aggttcttcc agaactccgc gcgagccggc
     5461 atgtcgtagg acaccagcat cggggtggcg ggcaggaggc cgacgtcagc ggcctgcttg
     5521 atgatgaggt acgcgggggt gccgaccccg atgttgatca ccacgtccgg cttccagctc
     5581 ttgatctcga gcagctgcgg ggtcaggtcc accaccgcgc gatcgaacac gatcgtcttc
     5641 agctccgctc cccccgcggt ggaggggaag agcttcttgg tgtcctcgac caggcccacc
     5701 ccgtagtcgg tgttctccgc gaggagcgcg accttcttga agcccttgtc cttgatccag
     5761 cggttccaga gctgcacgcg gtcggtgtcg atgacgtggg tgcggaagct gtagttgagg
     5821 tgcttctcgg tgaaggcgcg cgccgaggcc tgggtcgcga agatcggcac cttgaactgc
     5881 tcggccaggt cctggaccgc cagcgccacc gagctgtgga actggcccag caccgccacc
     5941 accccgtcct gggtcgcgag cttgcggaag ccggccgcgc ccttctcggg cgtgccggaa
     6001 tcgtcctcga tcaccagctc gatcttgcgc ccgcccagga tgcccttggc gttgatctcg
     6061 tcggccgcca tcttggcgcc gcgcacgatc agctggccgg cggcgggatc acccggaggc
     6121 gacagcgggg tcagcacgcc gatcttcacc ggcttctggg cgagggccgg cggggccagc
     6181 acggccaggg tcaagagagc cagcacgatg gcagtcaggc gtctactcat gcgttgcatc
     6241 ctccgctcac gctacgtggg tgtggaagga caggagaacc gcatcgtatg cgtcggggcc
     6301 gggccgcgtc aagcttcgcg ccccgcggtc agccgcggcc cagccgctcc agcagggtcg
     6361 cgtcggc
//
LOCUS       DATMCV010000704         9051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_571309, whole genome shotgun sequence.
ACCESSION   DATMCV010000704 DATMCV010000000
VERSION     DATMCV010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9051
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571309"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VNU03_16120"
     CDS             <1..780
                     /locus_tag="VNU03_16120"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWP76049.1"
                     /translation="RVRKAIDTYRFNDAASAVYQFLWHEYCDWYLEIAKRSLYQPESP
                     AARAVTQRTLVETLEATLRLLHPFMPFISEELWQRLPHQGDSIMIAAFPKASRKGRDP
                     EAERLMAPVLDFVSAIRTIRSESRISPAVELAVSVKPAGPEVAAALAAGAAVIGSLAR
                     AAITVSADGARPANSAMAATPSGDVFVRLEGVVDFEAERQRLRKEVERARKEIAFLEG
                     KLGRPDFVERAPAEVVERERARLTEQRETEQKLAASLAALG"
     gene            790..1563
                     /locus_tag="VNU03_16125"
     CDS             790..1563
                     /locus_tag="VNU03_16125"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWP76050.1"
                     /translation="MTRPDQPPLHFTVTALGIVDSTQSEVQRRAVAGAREGTVVTALH
                     QRAGRGRRGHEWWDAPGESLLFSVLLRPDREPAETPQLSLVGGLAVAEALAASTGVSA
                     RIRWPNDLLIDGRKVCGILAEAVSGSAARAHHVILGIGINLRQSAFPESLAERATSLR
                     LVTGRDHDAAALLAAVLARLEARYAVWRGGGFAPLREAWLAHSTLPGQPVRLPDGATG
                     RAEDVGADGVLLARAGDGRLVRVVSGAAVEEGTVHAAGH"
     gene            1547..2326
                     /locus_tag="VNU03_16130"
     CDS             1547..2326
                     /locus_tag="VNU03_16130"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012772073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWP76051.1"
                     /translation="MLLVIDVGNTNTSLGIYEAGRLRASWRLTTRREQTADEWGVFIQ
                     TLLATRGIEQQHITGVAISNVVPPIQQALEWMCDRYFGVSPLTVEPGVTADLPLNVDV
                     PREVGADRVVSCVAGIALYGTPLIVVDFGTATRFDCVNARGEFIGGAIAPGINTAAEA
                     LVSRAARLFRVELVRPKDAIGRHTVTNIQSGMIYGWAGLVDGLVERMRREMEGDVKVV
                     GTGGLVAQMREVVQCLQFVNPDLKLEGLRMLWERAHPGTTM"
     gene            2483..3643
                     /locus_tag="VNU03_16135"
     CDS             2483..3643
                     /locus_tag="VNU03_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HWP76052.1"
                     /translation="MGMWVGRGRKARAAYGFDDIALVPGALTINPSEVDISWELCGHR
                     FPIPIIAAAMDGVVSPSFAIEMGRVGGLAVLNLEGIFSRYENPDAVLDRITSASLEEA
                     TKIIQGIYSEPIKEELIHKRISEIKKGGGPVLVSSIPQRAERFAKIAEEAGADFFVVQ
                     STVTTARHVASEYTPVDLRQLKKHLSIPLIVGNVVTYEACLELMGCGVDALLIGVGPG
                     AACTSREVLGLGVPQVTATADSAAARDFYYKKTGRYVPIITDGGMTTGGDICKAFASG
                     ADAVMIGSAFARAKEAPGRGYHWGMATPHSNLPRGTRIRVGVAGSLEQILFGPAFTED
                     GTLNLVGAIRTCMGSVGAMNIRELQQTELIIAPSIKTEGKVFQRAQKLGTPR"
     gene            3651..5195
                     /gene="guaA"
                     /locus_tag="VNU03_16140"
     CDS             3651..5195
                     /gene="guaA"
                     /locus_tag="VNU03_16140"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWP76053.1"
                     /translation="MPSRVAILDFGGQYTQLIARRIRELGVYSEILPCTEPVERVLAG
                     GYTAIVLSGGPSSVYEDDAPLPDKRLLESGIPLLGICYGMQAMGYLMGGHVVPASVRE
                     YGKAHLRLLGREGLLAGVQPEADGLVTVWMSHGDTVLAPPPGFTSLAATDNCPVAAMA
                     DPARRIFAVQFHPEVAHTPQGKTVLKNFLDAAGATADWSMPSFVDSAVDGIRRQVGKD
                     PVLCALSGGVDSSVVAALIHRAVGDQLTCLFVDNGLLRKGEVDGVVRTFRDAFKMNLI
                     HVDASKRFIDKLEGISDPEVKRKTIGAEFIAVFEDEARKLGHIPWLAQGTLYPDVIES
                     VSFKGPSATIKTHHNVGGLPPNMRFKLVEPLRELFKDEVRELGALLGLPDEIIWRQPF
                     PGPGLAIRVLGDVTEERLALLREADAIVQEEVRHAGLERQVWQAFAVLLPIRTVGVMG
                     DFRTYAQVIALRAVTSLDAMTADWARLPYDLLGKISSRIINEVKGVNRVVYDISSKPP
                     STIEWE"
     gene            5401..>9051
                     /gene="dnaE"
                     /locus_tag="VNU03_16145"
     CDS             5401..>9051
                     /gene="dnaE"
                     /locus_tag="VNU03_16145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWP76054.1"
                     /translation="MKHADFVHLHVHSEYSLLDGAARLEKLVDRAKALKFPALALTDH
                     GNLFGAVDFYTACSKAGVKPILGCELYVAPGSRFERSSQDGGYEGASHCTVLARTAAG
                     YANLMKLVSKAYLEGYYYKPRVDRELLAQHADGLLVLSGCLNSEVSRQLSAGDEAKAL
                     QTAGWYQEVFGKDYYFMEVQSHGLEQQVAVTEGTVKIARAIGAPLCGTNDSHYLEADH
                     SRAHEALLCIQTGTTMSDPNRWKFSSNEFYVKSADEMKAVFKDLPEAYRNTIAVAERC
                     NVDLQFGQFHLPNYQVPEGYTLDSYLEHLALEGLARRYGGSPGDAIVERLRYELGVVS
                     KMGFSGYFLVVWDFIAYARRQGIAVGPGRGSSAGSLVAYCLGITNVDPIRYGLIFERF
                     LNPERISMPDMDIDFADDRRDEVIRYVVDRYGADRVAHIITFGTMGAKAVIRDVARVL
                     GFSYGEADRIAKLVPGFPLNISLDEALEKAPPLAEQVKRDQRVGEMWGVARALEGCTR
                     HASVHASAVVISDEPLMDRVPLYKDPKRPELITGYAMGPIEKLGLLKMDFLGLKTLTV
                     ITDALRLIKEARGISLDADALPLDDAGTYQLLTEARTFGVFQLESAGMRDALKRLKPQ
                     RIEDIIAMVALYRPGPMDLIEDFVNRKHGRAPIAYEHPLMERHLQETYGIMIYQEQVM
                     KLAADLAGFSLGEADTLRKAMGKKDRELMAQQREKFVSGCKANKIEVKKAERIWDLIE
                     KFAGYGFNKCLTAETRIEMADGSHKAITEIRAGDLVLTKDGPYRARAVRPSGVRRVGR
                     LHLANRMSVRCTPDHPIFTARGWVNAEDLAARDLVAVLRRLAPGESEDLVPVEVYGEA
                     LGESLTKVYATVEAGCRALRVPWKRPMRRGTLARLAVRFDSPALHALAESPVGWSRPR
                     GFALEGEEATYDFEVPGAASFIANGIAVHNSHAACYGVVAYQTAYLKANYPTEFMAAL
                     LTSEMEKTDKIVQYVEETRAMGLRVEPPDVNVSRAQFTVSGDAIHFGLAAIKNVGATA
                     IDSIVKVRETDGAFAALDDFCARVDLRLVNRRVIESLIKAGAFDSLRSTRAGLLASLD
                     QAMEGGQRRQRDREEGQVSLFDALGGGDTPKVATVASPAARVPEWPQEEMLAFEREVL
                     GFYLSGHPLEQYREVVRRIGALNAADLAARATGSRVLLLGQVSAFTESATKSGNRMAF
                     ATLELVDGSVPLT"
BASE COUNT         1395 a         3137 c         3217 g         1302 t
ORIGIN      
        1 cgggtgcgca aggcgatcga cacctaccgg ttcaacgacg cggcctccgc ggtctaccaa
       61 ttcctctggc acgagtactg cgactggtac ctcgagatcg ccaagcggag cctctaccag
      121 ccggagagcc cggccgcccg cgcggtcacc cagcgcacgc tggtcgagac gctcgaggcc
      181 acgctgcggc tcctccaccc cttcatgccg ttcatctccg aggagctgtg gcagcggctg
      241 ccgcaccagg gcgattccat aatgatcgcc gcattcccta aggcctctcg caaggggcgc
      301 gatccggagg cggagcggct catggcgccg gtgctcgact tcgtgagcgc gatccggacc
      361 atccggagcg agagccggat ctcgcccgcg gtggagctgg cggtgagcgt gaagccggcg
      421 ggtccggagg tggccgccgc gctcgcggcc ggcgcggcgg tgatagggag cctggcgcgg
      481 gccgcgatca cggtgtccgc cgatggagcc cgcccggcca actccgcgat ggcggccacc
      541 ccgagcggcg acgtgttcgt gcgcctcgag ggcgtggtgg acttcgaggc cgagcgccaa
      601 cgcctgcgca aggaggtgga gcgcgcgcgg aaggagatcg ccttcctcga gggcaagctc
      661 ggccgcccgg acttcgtgga gcgcgccccc gccgaggtgg tggagcgcga gcgcgcgcgg
      721 ctgaccgagc agcgggagac cgagcagaag ctcgcggcca gcctggccgc gctcggctag
      781 cccttcacgg tgaccaggcc cgaccagccg ccgttgcact tcacggtgac cgcgctgggc
      841 atcgtcgact ccacgcagtc cgaggtccag cgccgcgcgg tggcgggcgc gcgcgagggc
      901 acggtggtga ccgcgctcca ccagcgggcc ggccgcggcc ggcgggggca cgagtggtgg
      961 gacgcgccgg gcgaatccct cctcttctcg gtgcttcttc gccccgaccg cgagcccgcg
     1021 gagacgcccc agctctccct ggtgggcggc ctcgcggtcg cggaggcgct cgccgcgtcg
     1081 accggcgtga gcgcacgcat ccgctggccc aacgacctcc tgatcgacgg tcgcaaggtg
     1141 tgcggcatcc tggccgaggc ggtgtccggg tccgccgcgc gggctcacca cgtgatcctc
     1201 ggtatcggaa tcaacctccg gcagtccgcg tttccggagt ctctcgccga gcgggcgacg
     1261 tcgctccggc tcgtcaccgg ccgcgatcac gacgcggcgg cgctcctggc cgcggtgctc
     1321 gcgcggctcg aggctcgcta cgccgtctgg cggggcgggg gattcgctcc gctgcgggag
     1381 gcgtggctcg cccactcgac gctgccgggg cagccggtgc gcttgcccga cggcgcgacg
     1441 ggccgggccg aggacgtggg agcggacggg gtcctgctgg cccgcgcggg agacggacgg
     1501 ctggtgcgcg tcgtctcggg cgccgcggtc gaggagggga cggtccatgc tgctggtcat
     1561 tgacgtcggc aacaccaaca cctcgctcgg catctacgag gcggggcggc tgcgcgcgtc
     1621 ctggcgcctc accacgcggc gcgagcagac cgcggacgaa tggggcgtct tcatccagac
     1681 cctgctcgcc acccgcggca tcgagcagca gcacatcacc ggagtggcga tctccaacgt
     1741 ggtgccgccc atccagcagg cgctcgagtg gatgtgcgac cggtacttcg gggtgagtcc
     1801 cctcacggtg gagcccggag tcaccgccga tcttcccctc aatgtcgacg tcccgcgtga
     1861 ggtcggggcg gaccgggtgg tgagctgcgt ggccggcatc gcgctctacg ggaccccgct
     1921 catcgtggtg gacttcggca ccgccacccg cttcgactgc gtgaacgctc gcggcgagtt
     1981 catcggcggc gcgatcgcgc ccggtatcaa caccgcggcg gaggcgctcg tctctcgcgc
     2041 cgcgcgcctc ttccgcgtcg agctggtgcg cccgaaggac gccatcgggc ggcacaccgt
     2101 gaccaacatc cagtccggca tgatctacgg ctgggccggg ctcgtggacg ggctggtcga
     2161 gcggatgcgg cgggagatgg agggcgacgt gaaggtcgtc ggcaccggcg gtctggtggc
     2221 ccagatgcgc gaggtggtgc agtgcctgca gttcgtgaac cccgatctca agctcgaggg
     2281 cctgcgcatg ctctgggagc gggcccaccc cggcacgacc atgtaaaccg cgggtgaacc
     2341 ccggttgaca cccggggcga gccgctcgta tacttcaccc ctacctacag taggtcgacg
     2401 cggacggttc tctacatacg gggtatcggc ggcgggcaag cggcgccggg cccaggccgg
     2461 gtgaggccag gaggcggggc gtatggggat gtgggtcggg cgagggcgga aggcccgggc
     2521 agcgtacggc ttcgacgata tcgcgctggt tcccggcgcc ctgaccatca accccagcga
     2581 ggtggacatc tcctgggagc tctgcgggca tcgattcccg atcccgatca tcgcggcggc
     2641 catggacggg gtggtcagcc cctcgttcgc catcgagatg gggcgggtcg gcgggctcgc
     2701 ggtgctcaac ctggaaggca tcttctcccg ctacgagaat cccgacgcgg tgctggaccg
     2761 catcacctcc gcgagcctgg aagaagccac caagatcatc cagggcatct acagcgagcc
     2821 gatcaaggaa gagctgatcc acaagcggat cagcgagatc aagaaggggg gcggcccggt
     2881 cctggtctcc tccatcccgc agcgggctga gcgcttcgcc aagatcgcgg aggaggcggg
     2941 cgccgacttc ttcgtggtgc aatccaccgt gaccaccgcc cgacatgtgg cgagcgagta
     3001 caccccggtg gacctccgcc agctgaagaa gcacctctcg atcccgctca tcgtgggtaa
     3061 cgtggtcacc tacgaggcgt gcctcgagct gatgggctgc ggcgtggacg cgctcctgat
     3121 cggggtgggg ccgggcgcgg cgtgcacgag ccgcgaggtg ctgggcctcg gcgtgcccca
     3181 ggtgaccgcc accgcggact cggcggccgc gcgggacttc tactacaaga agaccggccg
     3241 ctacgtgccg atcatcaccg acggcggcat gaccaccggc ggcgacatct gcaaggcgtt
     3301 cgcctcgggc gcggacgcgg tcatgatcgg ctccgccttc gcgcgggcca aggaagcgcc
     3361 gggccgcggc taccactggg gcatggccac cccgcactcg aaccttcccc ggggcacccg
     3421 catccgcgtg ggcgtcgcgg ggtcgctcga gcagatcctc ttcgggcccg cgttcaccga
     3481 ggacggcacg ctcaacctgg tgggcgccat ccgcacctgc atggggtcgg tcggcgccat
     3541 gaacatccgg gagctgcagc agaccgagct gatcatcgcg ccctcgatca agaccgaggg
     3601 caaggtcttc cagcgcgccc agaagctggg gacgccgcgg tagatccccg gtgcccagcc
     3661 gggtcgcgat cctcgatttc gggggccagt acacgcagct gatcgcccgc cgcatccgcg
     3721 agctgggcgt ctactccgag atcctgccgt gcaccgagcc cgtcgagcgc gtgctcgccg
     3781 gcggctacac cgccatcgtg ctctccggcg ggccctcgag cgtctacgag gacgatgccc
     3841 cgctgcccga caagcgcctc ctcgagtccg ggatcccgct gctcgggatc tgctacggca
     3901 tgcaggccat gggctacctg atgggtggcc acgtggtgcc ggcatcggtg agggagtacg
     3961 gcaaggcgca cctgcggctc ctcggccgcg aggggctgct ggccggcgtg cagccggagg
     4021 ccgacgggct cgtgacggtg tggatgagcc acggcgacac cgtgctcgcg cccccgccgg
     4081 gcttcacgag cctggccgcc accgacaact gcccggtcgc ggccatggcc gacccggccc
     4141 ggcggatctt cgcggtgcag ttccatcccg aggtcgcgca caccccgcag ggcaagacgg
     4201 tgctcaaaaa cttcctcgat gcggcggggg ccacggccga ctggtccatg ccgtcgttcg
     4261 tggactcggc ggtcgacggc atccggcgcc aggtcggcaa ggaccccgtg ctctgcgcgc
     4321 tgtcgggcgg cgtggactcc tcggtggtgg cggccctgat ccaccgggcg gtgggcgatc
     4381 agctcacgtg cctcttcgtg gacaacggcc tcctgcggaa gggcgaggtt gacggggtcg
     4441 tccggacctt ccgagacgcc ttcaagatga acctgatcca tgtcgatgca agtaagcgat
     4501 tcatcgataa gctggaaggt atctcggatc ccgaggtcaa gcggaagacg atcggggccg
     4561 agttcatcgc ggtgttcgag gacgaagccc gcaagctcgg tcacatcccg tggctggccc
     4621 aggggacgct ctacccggac gtcatcgagt cggtgtcgtt caagggtccg tcggccacga
     4681 tcaagaccca ccacaacgtg ggcggcctgc ccccgaacat gcgcttcaag ctggtcgagc
     4741 cgctgcgcga gctcttcaag gacgaggtcc gcgagctggg cgcgctcctc ggcctgccgg
     4801 acgagatcat ctggcggcag ccgttccccg ggccgggcct ggccatccgc gtgctgggcg
     4861 acgtcaccga ggagcgcctg gcgctcctgc gcgaggccga cgccatcgtg caggaggagg
     4921 tgcgccacgc cgggctcgag cggcaggtgt ggcaggcctt cgcggtgctg ctcccgatcc
     4981 gcaccgtggg cgtgatgggc gacttccgga cctatgccca ggtcatcgcc ctgcgggcgg
     5041 tgacgagcct cgacgccatg accgccgact gggcgcggct gccctacgac ctgctcggca
     5101 agatctcgag ccggatcatc aacgaggtca agggcgtcaa ccgcgtcgtc tacgacatct
     5161 cctccaagcc cccgagcacc atcgagtggg agtgaccccg gcgggcccgg ccacatcgac
     5221 cgggccggag ggcacgcgcg aggcaggtcg gatgagcgct tgcgcgaaag agcccaaccg
     5281 ccaaaccgaa gtcttccaac attcgagcta gagatgggca cgcgcgaggc aggtcggatg
     5341 agcgcttgcg cgaaggagcc caaccgccaa accgaagtct tccacattta ggctagataa
     5401 atgaaacacg cggacttcgt gcacctgcac gtgcactcgg agtacagcct gctcgacggg
     5461 gcggcccgcc tcgagaagct ggtcgatcgg gccaaggcgc tcaagttccc ggcgctggcc
     5521 ctcaccgacc acggcaacct cttcggcgcg gtggacttct acaccgcgtg ctccaaggcg
     5581 ggggtgaagc cgatcctcgg ctgcgagctc tatgtggccc cgggcagccg cttcgagcgg
     5641 tcgagccagg acggcggcta cgagggcgcg agccactgca cggtgctggc ccgcaccgcg
     5701 gccggttacg ccaacctcat gaagctcgtc tccaaggcct acctcgaggg ctactactac
     5761 aagccccggg tggaccgcga gctgctcgcc cagcacgcgg acgggctgct ggtgctctcc
     5821 ggctgcctga actccgaggt gagccgccag ctctcggccg gcgacgaggc caaggcgctc
     5881 cagaccgcgg gctggtacca ggaggtgttc ggcaaggact actacttcat ggaggtgcag
     5941 tcgcacggtc tcgagcagca ggtggcggtg accgagggga ccgtcaagat cgcccgcgcc
     6001 atcggggccc cgctctgcgg gaccaacgac tcgcactacc tggaggccga ccactcgcgc
     6061 gcccacgagg cgctgctctg catccagacc ggcaccacca tgagcgaccc gaaccggtgg
     6121 aagttctcga gcaacgagtt ctacgtgaag tcggccgacg agatgaaggc cgtcttcaag
     6181 gacctgcccg aggcctaccg caacaccatc gcggtggccg agcgctgcaa cgtggatctg
     6241 cagttcggcc agttccacct gccgaactac caagtgccgg agggctacac cctcgactcc
     6301 tacctcgagc acctggcact ggaggggctc gcgcgccgct acgggggatc accgggcgac
     6361 gcgatcgtgg agcgcctgcg ctacgagctg ggcgtggtct ccaagatggg cttctcgggc
     6421 tacttcctgg tggtgtggga cttcatcgcc tacgcccggc gccagggcat cgcggtgggc
     6481 ccggggcgcg gctcgtccgc cggctcgctc gtcgcctact gcctcggcat caccaacgtg
     6541 gaccccatcc gctacgggct catcttcgag cgattcttga accccgagcg gatctcgatg
     6601 ccggacatgg acatcgactt cgcggacgac cgccgtgacg aggtcatccg ctacgtggtc
     6661 gaccgctacg gggcggaccg ggtggcccac atcatcacct tcgggaccat gggggccaag
     6721 gcggtcatcc gcgacgtggc ccgcgtgctc ggcttctcct acggggaggc cgaccgcatc
     6781 gccaagctgg tgcccggctt cccgctcaat atttcgctgg acgaggccct ggagaaggcc
     6841 ccgccgctcg ccgagcaggt caagcgcgac cagcgggtgg gggagatgtg gggggtggcg
     6901 cgcgcgctcg agggctgcac ccgccacgcc tcggtgcacg cctccgcggt ggtcatctcc
     6961 gacgagcccc tgatggatcg ggtgccgctc tacaaggacc ccaagcggcc ggagctgatc
     7021 accggctacg cgatggggcc gatcgagaag ctcggcctgc tcaagatgga cttcctcggg
     7081 ctcaagaccc tgaccgtgat caccgacgcc ctccgcctca tcaaggaggc gcgcgggatc
     7141 agcctcgacg cggacgcgct cccgctcgac gacgcgggca cgtaccagct cctgaccgag
     7201 gcgcgcacct tcggggtgtt ccagctggag tcggccggga tgcgcgacgc gctcaagcgc
     7261 ctgaagccgc agcgcatcga ggacatcatc gcgatggtgg cgctgtaccg gccgggcccg
     7321 atggacctca tcgaggactt cgtcaaccgc aagcacggtc gcgccccgat cgcctacgag
     7381 cacccgctca tggagagaca tctccaggag acctacggga tcatgatcta ccaggagcag
     7441 gtcatgaagc tcgccgcgga cctggccggg ttctcgctcg gcgaggccga caccctgcgc
     7501 aaggcgatgg ggaagaagga ccgcgagctg atggcccagc agcgcgagaa gttcgtctcc
     7561 ggctgcaagg ccaacaagat cgaggtcaag aaggcggagc gcatctggga cctcatcgag
     7621 aagttcgccg gctacgggtt caacaagtgc ctgaccgccg agacccgcat cgagatggcc
     7681 gacggcagcc acaaggccat caccgagatc cgggccgggg acctggtcct caccaaggac
     7741 ggcccctacc gcgcgcgcgc ggtgcgcccg agcggggtgc ggcgggtggg gcggctccac
     7801 ctcgccaacc gcatgtcggt gcgctgtacc cccgaccatc cgatcttcac cgcgcgggga
     7861 tgggtgaacg cggaagacct ggccgctcgc gatctggtcg cggtcctgcg ccggctcgcg
     7921 cccggcgaga gcgaggatct ggtgccggtc gaggtctacg gggaggcgct cggcgagagc
     7981 ctgaccaagg tctacgcgac ggtcgaggcg ggctgccggg ccctgcgggt cccgtggaag
     8041 cgcccgatgc ggcgaggcac gctggcccgg ctggccgtgc gcttcgactc gccggccctc
     8101 cacgccctgg ccgagtcgcc ggtcggctgg tcccggccga ggggcttcgc gctcgagggc
     8161 gaggaggcga cctacgactt cgaggtgccg ggcgccgcga gcttcatcgc caacggcatc
     8221 gcggtccaca actcgcacgc cgcctgctac ggggtggtgg cgtaccagac cgcgtatctc
     8281 aaggccaact acccgaccga gttcatggcc gccctgctca cctccgaaat ggagaagacg
     8341 gacaagatcg tccagtacgt ggaggagacg cgggcgatgg gcctgcgggt ggagccgccg
     8401 gacgtcaacg tctcgcgcgc ccagttcacg gtgtcgggcg acgcgatcca cttcgggctc
     8461 gccgcgatca agaacgtggg cgccaccgcg atcgactcga tcgtgaaggt gcgcgagacc
     8521 gacggggcgt tcgccgccct cgacgacttc tgtgcccgcg tggacctgcg cctagtcaac
     8581 cggcgcgtga tcgagtccct catcaaggcg ggcgcgttcg actcgctccg cagcacgcgc
     8641 gcgggcctcc tcgcgtccct cgaccaggcg atggagggcg ggcagcggcg gcagcgcgac
     8701 cgggaggaag ggcaggtctc gctcttcgac gcgctcggcg ggggcgacac gcccaaggtg
     8761 gcaacggtgg cgtcgcccgc ggcgcgggtc ccggagtggc cccaggagga gatgctggcc
     8821 ttcgagcgcg aggtgctggg cttctatctc tccggccacc cgctcgagca gtaccgggag
     8881 gtggtgcgcc ggatcggggc cctgaacgcg gcggacctcg ccgcccgcgc gaccggctcg
     8941 cgcgtgctgc tgctggggca ggtgagcgcg ttcaccgaga gcgcgaccaa gagcggcaac
     9001 cgcatggcct tcgcgacgct cgaactggtc gacgggagcg tgccgctcac g
//
LOCUS       DATMCV010000705         3125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_973806, whole genome shotgun sequence.
ACCESSION   DATMCV010000705 DATMCV010000000
VERSION     DATMCV010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973806"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /gene="nrfD"
                     /locus_tag="VNU03_16150"
     CDS             <1..643
                     /gene="nrfD"
                     /locus_tag="VNU03_16150"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWP76055.1"
                     /translation="ALVPGWHSTLFAPFFVDGAIFSGFAMVLVLILPMRYLFNWYPYI
                     TDRHLDAMAKLTLVTSLVLTYFYTCEVFTAWYSGDHFERASVFAKASKQYAWAYWGMY
                     FCNCLVPLVLLSRKARTHVVTLYVVSIFVLIGMWLERFNIIVPGLAHDFYPYTWGTYY
                     PTVTDSAIIVGSFAWFFILFLGFIRVMPSLSIVEVKEVLPHPLKHAASHGGHP"
     gene            643..1149
                     /locus_tag="VNU03_16155"
     CDS             643..1149
                     /locus_tag="VNU03_16155"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HWP76056.1"
                     /translation="MATASVLGVFAHVDTTLQAIRDLRAKGFSDLTVYTPVPVEEIEH
                     EVEKVRPLSAVRLFTLVGALTGTATGFFITIWTSLKWELVTGGKAPVSIPPFVIIAFE
                     LTILLGGLATALAALVLGQLPKLKPSPTYDPRFSVDRFGVAVACAPDKVDAVQAVLST
                     AGAEEVRR"
     gene            1146..1652
                     /locus_tag="VNU03_16160"
     CDS             1146..1652
                     /locus_tag="VNU03_16160"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWP76057.1"
                     /translation="MMKWVFVLCLLLIGGIGAVLGGAVGYRWMHNMFYTQRVMPGEYN
                     FSMPTGSLPRNGGQLYHSPAERDAAAARRNPVTASPDSVRRGGELFTIYCTPCHGATG
                     KGDGPVSTKFVPPADLSNPDLQKVRTDGYWQSYLSAGGAVMPSYAEALTPEERWHVVN
                     YVRTLAKR"
     gene            1649..2791
                     /locus_tag="VNU03_16165"
     CDS             1649..2791
                     /locus_tag="VNU03_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76058.1"
                     /translation="MSTMIATPASPARPHPFLILLAVLGIVAFLYGVTRPEPARVWGI
                     YLVNLLFWSSFAITGPAVAGMIQVTEGRWSPSIKRIALTTAGFLPVSLVLFVVLFFGR
                     ATLYPWVTEPIPVKAAWLNVPFMALRIGLGSLLLYWVAMRLVKAILVEDQPGQDTQAA
                     IDRRYRLAAVLLVLYVVVLSLWGFDLLMSLDPRWYSGLFGGYYVVTSMYPGFGLVTYL
                     TIRSNERGVTHVAPTAIQDVAKLTFALSVFWMYFFFSQYLVIWYGNVPAETRFIARRF
                     FSQPWGGIAWAIFIIGWLIPFSYLLKRLTGRPPERHQVFKVILFFGWIAIFVERMLLV
                     FPALLRDNTVPIGPVEVLVTLGFFALFVLSRNWFIGRYRPVLNLSK"
     gene            2831..>3125
                     /locus_tag="VNU03_16170"
     CDS             2831..>3125
                     /locus_tag="VNU03_16170"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76059.1"
                     /translation="MISVEEALDRILSRVAVLGDERVSLIQALHRVLAEDVDSPLDMP
                     PWPASSMDGYALRSTDTSSADAASPARLTVAGRVPAGGMAERPIRPGEAFRIFT"
BASE COUNT          402 a         1122 c         1016 g          585 t
ORIGIN      
        1 ggccctggtg ccgggctggc actcgacgct cttcgccccg ttcttcgtcg acggcgccat
       61 cttctccggc ttcgcgatgg tcctggtgct gatcctgccg atgcggtacc tgttcaactg
      121 gtatccctac atcacggacc ggcacctcga cgcgatggcc aagctgaccc tggtcacgag
      181 cctggtgctg acctacttct acacctgcga ggtgttcacc gcgtggtaca gcggggacca
      241 cttcgagcgg gccagcgtgt tcgccaaggc gtccaagcag tacgcgtggg cctactgggg
      301 catgtacttc tgcaactgcc tggtgccgct cgtgctcctg agccggaagg cgcgcaccca
      361 cgtggtcact ctctacgtgg tgtcgatctt cgtgctgatc gggatgtggc tggagcgctt
      421 caacatcatc gtgcccggcc tcgcccacga cttctacccc tacacctggg gcacctacta
      481 tccgaccgtc accgactccg cgatcatcgt cggctcgttc gcctggttct tcatcctgtt
      541 cctgggcttc atccgggtga tgccgtcgct gtcgatcgtg gaggtgaagg aggtgctgcc
      601 ccatccgctc aagcacgcgg cgtcccacgg gggccacccc tgatggccac cgcgagcgtg
      661 ctgggggtct tcgcccacgt ggacaccacg ctgcaggcca tccgtgacct gcgcgccaag
      721 ggcttctccg atctcaccgt ctacaccccg gtcccggtgg aggagatcga gcacgaggtc
      781 gagaaggtgc ggccgctgtc cgcggtgcgt ctcttcaccc tggtgggcgc gctcaccggc
      841 accgccaccg ggttcttcat cacgatctgg acctcgctca agtgggagct ggtcaccggc
      901 ggcaaggccc cggtgtcgat cccgcccttc gtcatcatcg ccttcgagct gacgatcctg
      961 ctcggcggcc tcgccaccgc gctcgcggcc ctcgtcctcg ggcagctgcc caagctcaag
     1021 ccctctccca cctacgaccc gcgcttcagc gtggaccgct tcggggtcgc ggtggcgtgc
     1081 gcgcccgaca aggtcgacgc ggtccaggcg gtgctgtcga ccgcgggggc ggaagaggtg
     1141 cggcgatgat gaagtgggtc ttcgtcctct gcctgctcct catcggaggg atcggcgcgg
     1201 tcctgggcgg cgcggtcggg taccgctgga tgcacaacat gttctacacg cagcgggtga
     1261 tgccggggga gtacaacttc tcgatgccca ccggcagcct gcccaggaac ggcggccagc
     1321 tctaccactc gcccgcggag cgcgacgcgg cggcggcgcg ccgcaacccg gtgacggcca
     1381 gccccgactc ggtgcgccgg ggcggggagc tgttcaccat ctactgcacg ccgtgccacg
     1441 gtgccaccgg gaagggggac gggccggtct cgaccaagtt cgtgccgccc gccgacctct
     1501 ccaaccccga tctgcagaag gtgcggacgg acggctactg gcagagctac ctgtccgcgg
     1561 gtggggcggt gatgccgtcc tacgcggagg cgctcacccc cgaggagcgg tggcacgtgg
     1621 tcaactacgt ccgcaccctg gccaagcgat gagcacgatg atcgcgaccc ccgcgagtcc
     1681 ggcccggccc catccgttcc tcatcctgct cgccgttctc gggatcgtcg cgttcctgta
     1741 cggggtcacc cgtccggagc ccgcgcgggt ctggggcatc tacctcgtca acctcctctt
     1801 ctggtcgagc ttcgcgatca ccggccccgc ggtggccggc atgatccagg tgaccgaggg
     1861 gcgctggtcg ccgagcatca agcgcatcgc gctcaccacc gcgggattcc tgcccgtgtc
     1921 gctggtgctg ttcgtggtgc tcttcttcgg ccgggccacg ctctacccgt gggtgaccga
     1981 gcccattccg gtcaaggcgg cctggctcaa cgtgccgttc atggccctgc ggatcggcct
     2041 gggctcgctc ctgctctact gggtggcgat gcgcctggtg aaggcgatcc tggtggagga
     2101 ccagcccggt caggacaccc aggccgccat cgaccggcgg taccggctcg cggcggtcct
     2161 gctggtgctc tacgtggtcg tgctctcgct gtggggcttc gacctcctga tgtcgctcga
     2221 cccgcgctgg tacagcgggc tcttcggcgg ctactacgtg gtgacctcga tgtaccccgg
     2281 cttcggcctg gtgacctacc tcaccatccg ctccaacgag cgcggggtga cccacgtggc
     2341 tccgaccgcg atccaggacg tggccaagct caccttcgcg ctctcggtct tctggatgta
     2401 cttcttcttc tcgcagtacc tggtcatctg gtacggcaac gtgccggcgg agacccgctt
     2461 catcgcccgg cgcttcttca gccagccgtg gggcgggatc gcgtgggcca ttttcatcat
     2521 cggctggctg atcccgttct cctacctgct caagcgtctc accgggcgcc cgccggagcg
     2581 gcaccaggtc ttcaaggtga tcctgttctt cggctggatc gcgatcttcg tcgagcggat
     2641 gctgctcgtg ttccccgcgc tgctgcgcga caacacggtg ccgatcgggc cggtcgaggt
     2701 gctggtgacc ctcggcttct tcgccctgtt cgtgctcagc cggaactggt tcatcggccg
     2761 ttaccggccg gtcctgaacc tgtccaagta gcccgccgac cccctcgcgt cccgcggtag
     2821 aatgcggggc gtgatctccg tcgaggaagc gctcgaccgc atcctctccc gcgtcgcggt
     2881 cctcggcgac gagcgcgtgt cgttgatcca ggcgctccac agggtgctcg ccgaggacgt
     2941 ggacagcccg ctcgacatgc cgccgtggcc cgcctcctcg atggacggct acgccctccg
     3001 cagcacggac acgtcgagcg cggacgcggc gtcccccgcg cggctgacgg tggcggggcg
     3061 ggtgcccgcg ggcggcatgg cggagcgccc gatccgcccc ggcgaggcct tccgcatctt
     3121 caccg
//
LOCUS       DATMCV010000706         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_304600, whole genome shotgun sequence.
ACCESSION   DATMCV010000706 DATMCV010000000
VERSION     DATMCV010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304600"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VNU03_16175"
     CDS             <1..581
                     /locus_tag="VNU03_16175"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76060.1"
                     /translation="ALVATVGSLGAGVAASFALLRRRVAGGAGVAALLNAPLVFPGVV
                     VGVALLQFYAMVHLNGSLVGLALAHMVITVPYVVRAVTASLQGIDPELEHAARVLGAS
                     GPVAFFTVTLPLIRPGVAAGALFAFIVSFDNVPVSIFLLGAGQMTLPVKIFTAIEYGV
                     DPSIAAISTMLIVLTGVGLAVAERWIGFHRFV"
     gene            602..1372
                     /locus_tag="VNU03_16180"
     CDS             602..1372
                     /locus_tag="VNU03_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76061.1"
                     /translation="MATALEGRLAGRIALVSGAANGIGRASALRLAREGADLALIDRE
                     GDPLHAVAREVEAGGRQVLTITADWTDERAVLEAFATIRQRFGRIDVLFNNVGQSARE
                     RASEFHLSEEATWRFVIEVSLLATMRATRLAVEEMRSRRSGRIVNMSTESAFYGDVGF
                     VDYAAAKMGVVGFTRSLARELAPFHVNVNAVCPGAIRTRAHDRLPKEVIDKVRNSVPM
                     GYVAEPEDVAGVVAFLASDDARYITGQSILIDGGRWMI"
     gene            1411..2193
                     /locus_tag="VNU03_16185"
     CDS             1411..2193
                     /locus_tag="VNU03_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP76062.1"
                     /translation="MAGRLAGKIAVITGGGSGIGRACAVRFAMEGAKVCVADLDQAAA
                     KDTARRVEAAGQKSLALRVDTTDEVANDLMVEQCVEAFGAVDVLVAAAGVGSPRPEAG
                     SAKPYTMLTMPIEAFRSVIDVNLYGVLFSNRAVASFMVANGRGGSLINLGSIMSKMPS
                     TGGAYSVSKAGVWMATKCLAQELARHGIRVNAIGPGFIETPMTAPLRGDADRSRWAMG
                     MTPLGRYGTAEEVASTALFLASDDAAFFTGEILHPSGGVFVG"
BASE COUNT          315 a          788 c          901 g          359 t
ORIGIN      
        1 tcgcgctggt cgcgaccgtc gggtcgctcg gcgccggggt ggccgcgtcc ttcgcgctgc
       61 tccggcgccg ggtggccggc ggtgccggcg tcgccgcgct gctcaacgca ccgctggtct
      121 tccccggagt ggtggtgggg gtggccctgc tgcagttcta cgcgatggtc cacctgaacg
      181 gcagcctcgt ggggctggcg ctcgcccaca tggtgatcac ggtgccctac gtggtccggg
      241 cggtgaccgc gagcctccag gggatcgatc ctgagctcga gcacgccgcg cgcgtgctgg
      301 gcgcgagcgg gccggtcgcg ttcttcacgg tcacgctgcc cctgatcagg cccggcgtgg
      361 cggccggggc gctcttcgcg ttcatcgtgt cgttcgacaa cgtcccggtg tcgatcttcc
      421 tcctcggggc gggccagatg acgctgccgg tgaagatctt caccgccatc gagtacgggg
      481 tggacccgtc catcgcggcc atctcgacca tgctgatcgt gctcaccggc gttggcctcg
      541 cggtggccga gcgctggatc ggcttccacc gcttcgtctg attggagcca ggaggaccat
      601 gatggccact gccctcgaag gccggctcgc cggccgcatc gcgctcgtga gcggggccgc
      661 caacggcatc ggccgcgcca gcgccctgcg cctcgcccgg gagggcgcgg acctggcctt
      721 gatcgaccgc gagggcgacc cgctgcacgc ggtggcgcga gaggtcgagg cgggcggccg
      781 gcaggtgctg acgatcaccg cggactggac cgacgagcgg gcggtgctcg aggcctttgc
      841 caccatccgg cagcggttcg ggcgcatcga cgtcctcttc aacaacgtcg gccagagcgc
      901 ccgcgagcgg gcgagcgagt tccacctctc ggaggaggcg acgtggcgct tcgtcatcga
      961 ggtctcgctg ctcgcgacca tgcgggccac gcggctggcg gtcgaggaga tgcggagccg
     1021 ccgcagcggg cgcatcgtca acatgtcgac cgagtccgcc ttctacggcg acgtggggtt
     1081 cgtggactac gcggcggcga agatgggtgt ggtcgggttc acccgctcgc tggcgcgcga
     1141 gctggcgccg ttccacgtca acgtcaacgc ggtctgcccc ggcgccattc gcacccgcgc
     1201 ccacgaccgg ctgccgaagg aagtgatcga caaggtgcgg aacagcgtgc ccatgggcta
     1261 cgtggccgag cccgaggacg tggccggtgt cgtggcgttc ctggccagcg acgacgcgcg
     1321 ctacatcacc gggcagtcga tcctgatcga cggcggccgg tggatgatct gattcgaccg
     1381 ggaagcttca aacccacgga ggaggcgacc atggcgggac ggctcgcggg caagatcgcg
     1441 gtgatcaccg gcggtggcag cggcatcgga cgcgcgtgcg cggtccgctt cgcgatggaa
     1501 ggggcgaagg tgtgcgtggc cgacctggat caggccgcgg ccaaggacac cgcgcggcgg
     1561 gtggaggcgg ccgggcagaa gtcgctggcg ctgcgggtcg acaccaccga cgaggtggcc
     1621 aacgacctca tggtggagca atgcgtcgag gccttcggcg cggtggacgt cctggtggcc
     1681 gcggccggcg tggggagccc acgcccggag gccggctccg cgaagcccta caccatgctc
     1741 accatgccga tcgaggcctt ccgctcggtc atcgacgtga acctctacgg ggtgctcttc
     1801 tcgaaccgcg cggtggcgag cttcatggtg gctaacgggc ggggtgggag cctgatcaac
     1861 ctgggctcga tcatgtcgaa gatgccctcg accggtggcg cctacagcgt gagcaaggcc
     1921 ggcgtctgga tggcgacgaa gtgcctggcc caggagctgg cgcgccacgg catccgggtg
     1981 aacgcgatcg gaccgggctt catcgagacg ccgatgaccg cgccgctgcg gggcgatgcc
     2041 gatcggagcc gctgggccat gggcatgacc ccgctgggcc gctacggcac ggcggaggag
     2101 gtcgcgagca ccgcgctctt cctggcctcc gacgacgccg cattcttcac cggcgagatc
     2161 ctccacccct ccggcggcgt cttcgtcggc tagcccctca ccctgccctc tccccacagg
     2221 ggagagggtg accccgctac ccctctgccc ccgctctccc tctcccctca ggggagaggg
     2281 cggggtgagg ggtggggcag ggtgaggggt ggggcagggt gaggggtggg gcagggtgag
     2341 gggtggggca gggtgagggg tgg
//
LOCUS       DATMCV010000707         3620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_304703, whole genome shotgun sequence.
ACCESSION   DATMCV010000707 DATMCV010000000
VERSION     DATMCV010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304703"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VNU03_16190"
     CDS             complement(<1..315)
                     /locus_tag="VNU03_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine methyltransferase"
                     /protein_id="HWP76063.1"
                     /translation="MTGFALFDTAIGWCGIAWGPRGIVGVQLPEGSAPATRVRLLRRF
                     PDAGEAPPPPEARRAIGDITALLRGEPKTLDDVPLDMEGLPEFNRRVYEVARTIPWGA
                     TLS"
     gene            complement(312..761)
                     /locus_tag="VNU03_16195"
     CDS             complement(312..761)
                     /locus_tag="VNU03_16195"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HWP76064.1"
                     /translation="MSEKSALLHDADQAFTELRGAIEGLSDDEMRRVWLGSWGVREIL
                     IHISGWHDELIPALGRIGQGQAGYPAGTYDDFDAWNARFVDRRAGVKPVDVVAELEAS
                     HRAFVVAAASVPEALFGPGGAARGPFEGAGAGHYREHAAQIRQWRGQ"
     gene            complement(838..1155)
                     /locus_tag="VNU03_16200"
     CDS             complement(838..1155)
                     /locus_tag="VNU03_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76065.1"
                     /translation="MLLDPVLWLFVLSVGLLVVGVLGVVVATRRARLSERQGVPGVSP
                     DVGSDFARIHELQTQVKQLGDEYDRLAAERDELEAVLARLAVLLEQADRTAAGTSTSR
                     VAR"
     gene            complement(1219..1902)
                     /locus_tag="VNU03_16205"
     CDS             complement(1219..1902)
                     /locus_tag="VNU03_16205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP76066.1"
                     /translation="MATYVLVHGAYQGGWIWKPVVERLRSAGHLVYAPTLDGCAERHD
                     QVRPGITVGTHAQEVAQLLFYEDLRDVVLVGTSSGGMVICRAAELAGERIARLVFVDA
                     LALMPGERVADIVSRPSGDTTAVTTAPTRADAEGRMFADLEPATRAWALARYTPHPIA
                     ALEAPMELATFWDRAWTASVIRCRRARNPPEAHQRRTAERLKAPYAELDTGHYPMLSQ
                     PDELVRLLA"
     gene            complement(1907..2557)
                     /locus_tag="VNU03_16210"
     CDS             complement(1907..2557)
                     /locus_tag="VNU03_16210"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76067.1"
                     /translation="MHTARPGHIVICMSTIDPLAVRRLGERLAARGIALLDAPVSGGT
                     GRAASGELSIIVGGATETVAACQDLFRAMGARVFHMGALGQGLAMKLVNNMLIQVNTV
                     AVAEALVLGVKAGLDPRAIHDVVRVSTGNSWAFESSVPRILRRDFSPGGTVDIAFKDQ
                     ELETAFAKQLGVPILRANVTQQVYQMARAAGLNKEDGSAVIKVLEQLAGVRVGGEA"
     gene            complement(2527..2802)
                     /locus_tag="VNU03_16215"
     CDS             complement(2527..2802)
                     /locus_tag="VNU03_16215"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWP76068.1"
                     /translation="MAGTVGFVGLGAMGGPMAQNLLEHGFALAVHDVDPTKAALWRGR
                     GAAVAGSAADVSAQADRTICMVETTAQAEDVIAGSAASCTPPVPATS"
     gene            2917..>3620
                     /locus_tag="VNU03_16220"
     CDS             2917..>3620
                     /locus_tag="VNU03_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP76069.1"
                     /translation="MGDPGPTIEKLTPHIGAIVHGVDLARPLDEPTFKRVHDALIDNQ
                     VIFFRDQHLTPDQQKDFGRLFGELAVHPAAPGLVEGHPEILVVHADEKSKHVAGENWH
                     SDVSCDPEPPMGSILYMHELPPVGGDTLFASMYAAYEALSPPMKRLLEGMTAIHRGEH
                     VYRGRYGVKDEGRQFPEAEHPIVRTHPVSGRKALFVNRTFTTRIVQLSRSESAAVLSF
                     LYQHVETPEFQCRFRW"
BASE COUNT          505 a         1365 c         1258 g          492 t
ORIGIN      
        1 cgagagggtg gcgccccacg ggatcgtgcg ggcaacctcg tagacgcgcc ggttgaactc
       61 aggcagcccc tccatgtcga gcggcacatc gtcgagcgtc ttcggctcgc cgcgcaggag
      121 cgcggtgatg tcgccgatcg cgcgccgcgc ctcgggcggc ggcggcgcct cgcccgcgtc
      181 ggggaagcgg cgcagcagcc gcacgcgggt cgcgggcgcg ctcccctccg ggagctgcac
      241 gccgacgatc ccgcgcgggc cccaggcgat cccgcaccag ccgatcgcgg tgtcgaagag
      301 ggcgaagccg gtcattgccc tcgccactgg cggatctgcg cggcgtgctc gcggtagtgc
      361 cccgcgcccg cgccctcgaa gggaccacgc gcggccccgc ccggcccgaa cagcgcctcg
      421 ggcaccgacg ccgcggcgac gacgaaggcc cgatgagacg cctcgagctc cgccaccacg
      481 tcgaccggct tcacgcccgc ccggcggtcc acgaagcgcg cgttccatgc gtcgaagtcg
      541 tcgtaggtgc cggccgggta gccggcctgg ccctgcccga tgcgtccgag ggccgggatc
      601 agctcgtcgt gccagcccga gatgtggatc aggatctcgc gcacgcccca gctcccgagc
      661 cagacgcgac gcatctcgtc gtcgctcagc ccctcgatcg ctccccgtag ctcggtgaat
      721 gcctgatcgg cgtcgtgcaa cagcgcactc ttctcgctca tgggtccctc ccgggttggt
      781 gggtctcgct ttacgtgggc tgagaccccg gtcgggcggg gttgtgaaac ggcccggtca
      841 gcgggcgacg cgggaggtgg aggtgccggc ggcggtgcgg tccgcctgct ccagcaggac
      901 cgcgaggcgg gccagcaccg cctccagctc gtcgcgctcc gcggccagcc ggtcgtactc
      961 gtcgccgagc tgcttgacct gggtctgcag ctcgtggatc cgggcgaagt cggagccgac
     1021 gtcggggctc acgccgggca ctccctgccg ctcgctcagc cgggcccgcc gggtggcgac
     1081 cacgaccccc agcaccccca ccaccagcag gccgaccgag aggacgaaca gccagagcac
     1141 cgggtccagc agcatggcga acagtctacc ccgagccggg tccaggagct tcgttcagcc
     1201 tgatggggat cggccgcctc aggcgagcag gcggaccagc tcgtcgggct ggctcagcat
     1261 cgggtagtgc ccggtgtcga gctcggcgta gggcgccttg agccgctccg cggttcggcg
     1321 ctgatgggcc tcgggggggt tgcgggcgcg ccggcagcgg atcaccgagg cggtccacgc
     1381 gcgatcccag aacgtggcca gctccatggg ggcctcgagc gccgcgatcg ggtgcggggt
     1441 gtagcgcgcg agcgcccacg cccgggtggc cggctcgagg tcggcgaaca tgcgcccctc
     1501 cgcgtcggcc cgggttggcg cggtggtcac cgcggtagta tccccggacg gtcggctcac
     1561 gatgtcggcg acccgctcgc cgggcatgag ggccagcgcg tccacgaaca cgagccgggc
     1621 gatgcgctcg cccgccagct cggcggcccg gcagatgacc atgccgcccg agctggtgcc
     1681 gaccaggacc acgtcgcgca ggtcctcgta gaacagcaac tgcgcgacct cctgagcgtg
     1741 ggtgccgacc gtgatcccgg gacggacctg gtcgtgccgc tcggcgcagc cgtcgagggt
     1801 cggggcgtac acgaggtgcc cggctgagcg gagccgctcc accaccggct tccagatcca
     1861 gccgccctgg tacgcgccgt gaacgagcac gtaggtcgcc atcgcgctag gcctccccgc
     1921 cgacgcgcac gccggccagc tgctcgagca ccttgatcac cgcggagccg tcctccttgt
     1981 tgagcccggc cgcccgcgcc atctggtaga cctgctgggt cacgttggcg cgcaggatcg
     2041 gcaccccgag ctgcttggcg aacgcggtct cgagctcctg gtccttgaac gcgatgtcca
     2101 ccgtgccgcc gggagagaaa tcgcgccgga ggattcgcgg cacgctggac tcgaacgccc
     2161 agctgttgcc ggtgctgacc cgcaccacgt cgtggatcgc ccgggggtcg agccccgcct
     2221 tcacgccgag cacgagggcc tcggccaccg cgaccgtgtt cacctggatc agcatgttgt
     2281 tcacgagctt catcgcgagc ccctgcccga gcgcgcccat gtggaagacg cgcgccccca
     2341 tcgcccggaa gagatcctgg cacgcggcga ctgtctccgt cgcgccgccc acgatgatcg
     2401 acagctcgcc cgacgccgcg cgcccggtgc cgccgctgac cggggcgtcg agcagcgcga
     2461 tcccccgcgc cgcgagccgc tcgccgagcc ggcgcaccgc cagcggatcg atggtgctca
     2521 tgcagatcac gatgtggccg ggacgggcgg tgtgcatgat gccgcgctcc ccgcgatgac
     2581 gtcctcggcc tgtgcggtgg tctccaccat gcagatcgtc cggtcggcct gcgcgctcac
     2641 gtcggcggcg gagccggcca ccgcggcgcc tcgcccgcgc cagagggcgg ccttcgtcgg
     2701 atcgacgtcg tgcaccgcca gggcgaagcc gtgctcgagg aggttctgcg ccatcgggcc
     2761 gcccatggcc cccaacccca cgaatccgac cgtgcccgcc atgtcccgcg tcctcccggc
     2821 ccctctctac tacgatcgag acgggttgtc gatgccctcc ggcgcgcttg acgacgcgcc
     2881 gggggcgggg cacgatcggg cccacaggag gcacccatgg gagatccggg cccgaccatc
     2941 gagaagctca cgccccacat cggcgcgatc gtccacggcg tcgacctcgc ccgaccgctc
     3001 gacgagccca ccttcaagcg ggtgcacgac gcgctcatcg acaaccaggt gatcttcttc
     3061 cgcgaccagc acctcacccc ggatcagcag aaggacttcg ggcgcctctt cggcgagctc
     3121 gcggtgcatc ccgccgcgcc ggggctggtg gaggggcacc ccgagatcct ggtggtgcac
     3181 gccgacgaga agtccaagca cgtggcgggc gagaactggc actccgacgt ctcgtgcgac
     3241 cccgagcccc ccatggggag catcctctac atgcacgagc tgccgccggt cggaggcgac
     3301 accctgttcg cgagcatgta cgcggcctac gaggcgctct ctccgccgat gaagcggctg
     3361 ctcgagggca tgaccgccat ccaccggggc gagcacgtct accgcggacg ctacggcgtg
     3421 aaggacgagg gccggcagtt cccggaggcc gagcatccga tcgtgcgcac ccacccggtc
     3481 tcggggcgca aggccctctt cgtcaaccgg accttcacca cccggatcgt ccagctctcc
     3541 cgcagcgaga gcgcggcggt gctctcgttc ctctaccagc acgtcgagac ccccgagttc
     3601 cagtgccgct tccggtggca
//
LOCUS       DATMCV010000708         4305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_637226, whole genome shotgun sequence.
ACCESSION   DATMCV010000708 DATMCV010000000
VERSION     DATMCV010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637226"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VNU03_16225"
     CDS             <1..1428
                     /locus_tag="VNU03_16225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWP76070.1"
                     /translation="TDLPWRRSTQHLVMASFVTPPFLGAFAWVMLAGPNAGYLNRLYR
                     DWTGAADPLLNIFSMPGLIFVVAIYTFPYVYIMIANTLGLIASDLEDAASILGAGRFQ
                     VARTITLPMVLPAILSGFILAVLQALALFGSPAILALPAGFHTVTTQLWAFFQYPPRV
                     EMAAAFSIPLLLATALLLLAQKRLLGRRGYAAVGGKGGQRRVIRLGRWRYPALGACLV
                     VLGAAVFLPYGILLKAAFSRAWAQPLTPENFTLENWSFTFLYSTTQSAIVNTIELGVL
                     TACIGAALATLLAYVTQRRLVAGHQVLAFLAVAPVVIPGVVLAVALFVAYTRPPFLLY
                     GTLWILFLAYLTKEMPVGYSQSEATLRGIHPELEEAGRILGAGRFRVLHEITAPLARS
                     GIIATWCFIFIGVIRELSASIILFTPNTKVMSVVIFDLKEEGQFGAIAVLGVFMLAMT
                     FVTVVVMQTVLGRDVMGGRQAGGAG"
     gene            1428..2537
                     /locus_tag="VNU03_16230"
     CDS             1428..2537
                     /locus_tag="VNU03_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76071.1"
                     /translation="MPGITITGLTKRFGDVGAVVGLDLTVRPGELVALLGPSGCGKTT
                     TLRLVAGFLTPEGGEIRVGDRVLSSAAVVVPPERRRMAMIFQSYALWPHMTVAQNVAY
                     GLRFAGVARGDRERRVDEMLRAVQLAGYGARYPGELSGGQQQRVAVARALVVEPEILL
                     LDEPLSNLDASLREEMRFEIRRLHERFAITTLYVTHDQAEAMVISDRVAVLREGRVAQ
                     VGPPAELFEHPRTRFVAEFVGKTNLIDAVADGSGGVTRGTLRLRVAAKSLVPRAPVVV
                     SIRPHQITLGPPARDADDANVLAGTVVRASYLGDAIDYQIAIDGTDVVLRVTGAAPAR
                     SRPGDAVALTVPVSACVPLPATDDPGPSDAGRGPT"
     gene            complement(2534..3652)
                     /locus_tag="VNU03_16235"
     CDS             complement(2534..3652)
                     /locus_tag="VNU03_16235"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HWP76072.1"
                     /translation="MTAPRPPFRADHVGSLLRSPALKARRRDHEDGRIDAAALAAAED
                     EAVREAVRLQEAAGLEGITDGELRRKSWHMDFLTRLGGLASEGRALPVTFHGRSGDVN
                     FTRPDLRIDARVGRPAPIFVDAFRFLRSVTTRTAKISIPSPCMLYSQVGRANISRAVY
                     PDLDRFVEDTAAAYRAEIADLYAAGCRYLQLDDVSLAYLCDEGMRADGRARGEDPDAV
                     LALSVKLVQATLRDRPRDLSVCTHLCRGNFRSSWRAQGGYEKVAETIFNQMPYDGFFL
                     EFDDARSGDFSPLRHAPPHVRVVLGLVSSKVGALEPKRDLIGRIEEASKFLPLDQLCL
                     SPQCGFSSTLEGNDVSVEQQEAKLRLCAEVAREVWGGL"
     gene            complement(3649..>4305)
                     /locus_tag="VNU03_16240"
     CDS             complement(3649..>4305)
                     /locus_tag="VNU03_16240"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWP76073.1"
                     /translation="FHGLGGSHEGAAVRAVDPDGRVAAAIPVRHVIGCVVHATCSVVE
                     PGLGRHGFGRGLIIGEPGGGTSARVSALAERLEAAGFETTVSLRIQADVWYKLWGNMT
                     MNPMSALTGATCDRLLDDPLLDRFTLAIMAEAAAIGEKIGCPIRQSGEERNAVTRKLG
                     AFKTSMLQDVEAGKPLEIDALLTAVSEIGGRVGVGTPSTDALLGLTRVFAAGRGLYRP
                     "
BASE COUNT          550 a         1607 c         1502 g          646 t
ORIGIN      
        1 accgacctgc cgtggcgtcg cagcacccag cacctggtca tggcctcgtt cgtgactccg
       61 cccttcctgg gcgccttcgc gtgggtcatg ctcgccggcc ccaacgcggg ctatctcaac
      121 cggctctacc gtgactggac cggcgccgcc gatccgctgc tcaacatctt cagcatgccg
      181 gggctgatct tcgtggtggc gatctacacg ttcccctacg tctacatcat gatcgccaac
      241 accctgggcc tcatcgcctc cgacctcgag gacgccgcct ccatcctggg cgcgggccgc
      301 ttccaggtgg cgcggaccat caccctgccc atggtgctgc ccgcgatcct gagtggcttc
      361 atcctggccg tgctgcaggc cctcgccttg ttcggctccc ccgccatcct ggcgctgccc
      421 gccggcttcc acaccgtgac cacccagctc tgggccttct tccagtaccc gccgcgcgtc
      481 gagatggcgg ccgccttctc gatcccgctc ctgctcgcca ccgccctgct gctgctcgcg
      541 cagaagcggc tgctgggccg gcgcggctac gccgcggtgg gaggcaaggg cgggcagcgc
      601 cgggtgatcc ggctcgggcg gtggcgctat cccgcgctcg gcgcgtgcct cgtggtgctg
      661 ggcgccgcgg tgttcctgcc ctacggcatc ctgctcaagg ccgccttctc gcgcgcgtgg
      721 gctcagccgc tcaccccgga gaacttcacg ctagagaact ggtcgttcac gttcctctac
      781 agcaccaccc agtccgccat cgtcaacacg atcgagctgg gggtgctcac cgcgtgcatc
      841 ggggccgccc tcgcgaccct gctcgcctac gtcacccagc ggcgcctcgt cgcgggccac
      901 caggtgctcg ccttcctcgc ggtggccccg gtggtcatcc cgggcgtggt gctggcggtg
      961 gcgctcttcg tggcctacac gcgcccgccc ttcctgctct acggcacgct ctggatcctc
     1021 ttcctcgcct acctgaccaa ggagatgccg gtcggctact cgcagtccga ggcgaccctc
     1081 cgcggcatcc acccggagct ggaggaggcg ggccgcatcc tgggggccgg gcgctttcgg
     1141 gtgctgcacg agatcaccgc cccgctcgcc cgcagcggga tcatcgcgac ctggtgcttc
     1201 atcttcatcg gagtgatccg cgagctgtcc gcgtcgatca tcctgttcac cccgaacacc
     1261 aaggtcatgt cggtggtcat cttcgatctg aaggaggagg gccagttcgg ggccatcgcg
     1321 gtgctcgggg tcttcatgct cgcgatgacc ttcgtcacgg tggtggtgat gcagaccgtg
     1381 ctcggccgcg acgtgatggg cggccggcag gcgggcgggg ccggctgatg cccgggatca
     1441 cgatcaccgg cctcaccaag cgcttcggcg acgtcggcgc ggtggtcggc ctcgatctca
     1501 cggtgcggcc gggcgagctg gtggccctgc tcggcccgtc cggctgcggc aagaccacca
     1561 ccctgcgtct ggtcgccggc ttcctcaccc ccgagggagg cgagatccgg gtgggcgacc
     1621 gcgtgctctc gtcggccgcg gtggtcgtcc cccccgagcg gcgccgcatg gcgatgatct
     1681 tccagagcta cgccctgtgg ccgcacatga cggtggccca gaacgtggcc tacgggctcc
     1741 gcttcgcggg cgtcgcgcgc ggcgatcgcg agcggcgggt ggacgagatg ctgcgcgcgg
     1801 tgcagctcgc gggctacggc gcgcgctacc cgggcgagct gtcgggcggc cagcagcagc
     1861 gggtggcggt ggcccgggcc ctggtggtgg agcccgagat cctgctgctc gacgagccgc
     1921 tcagcaacct cgacgcgagc ctgcgcgagg agatgcggtt cgagatccgt cggctccacg
     1981 agcgattcgc catcaccacg ctgtacgtca cccacgacca ggcggaagcg atggtgatct
     2041 cggaccgggt cgcggtgctg cgcgaggggc gcgtcgcgca ggtcgggcca ccggcggagc
     2101 tcttcgagca cccgcgcacc cgcttcgtgg ccgagttcgt gggcaagacc aacctcatcg
     2161 acgcggtggc cgacggctcc ggcggggtga cccgcgggac cctgcggctg cgggtggccg
     2221 cgaagagcct cgtcccgcgg gctccggtgg tggtctcgat ccgcccgcac cagatcacgc
     2281 tcggcccgcc cgcccgcgat gccgacgatg ccaacgtgct ggccggcacc gtggtgcgcg
     2341 cgagctatct cggggacgcg atcgactacc agatcgcgat cgacggcacc gacgtggtcc
     2401 tgcgcgtgac cggcgcggcg cccgcgcgct cgcggcccgg cgacgcggtc gcgctgaccg
     2461 tacccgtcag cgcgtgtgtc ccgctgcccg ccaccgacga ccccggcccg tccgacgctg
     2521 gccggggccc gacctagaga ccgccccaga cctcgcgcgc cacctcggcg cagaggcgaa
     2581 gcttcgcctc ctgctgctcc accgagacgt cgttgccctc gagcgtcgag gagaacccgc
     2641 actgggggct caggcacagc tgatcgaggg gcaggaactt cgaggcttcc tcgatccggc
     2701 cgatgaggtc gcgcttcggc tcgagcgccc cgaccttgct gctcacgagc cccagcacca
     2761 cccgcacgtg cgggggcgcg tggcggagcg gcgagaaatc gccggagcgc gcgtcgtcga
     2821 actcgaggaa gaagccgtcg tagggcatct ggttgaagat ggtctccgcc accttctcgt
     2881 acccaccctg ggcccgccag ctcgaccgga agttcccgcg gcagaggtgg gtgcacaccg
     2941 agaggtcgcg cggccgatcc cgcagcgtgg cctgcacgag cttcaccgag agggccagca
     3001 ccgcgtccgg gtcctcaccg cgcgcccgcc cgtccgcccg catgccctcg tcgcagaggt
     3061 aggccaggct cacgtcgtcg agctgcagat accggcagcc cgccgcgtag aggtcggcga
     3121 tctcggcgcg gtaggcggcc gcggtgtcct ccacgaagcg gtcgagatcc gggtacaccg
     3181 cgcggctgat gttggcgcgc ccgacctggg agtagagcat gcagggcgac gggatgctga
     3241 tcttcgcggt gcgggtggtg accgagcgca agaagcggaa ggcgtcgacg aagatcgggg
     3301 cgggccggcc cacgcgggcg tcgatccgga gatccgggcg ggtgaagttc acgtcgcccg
     3361 atcgcccgtg gaacgtcacc ggcagcgcgc ggccctcgct ggccaggccg cccaggcggg
     3421 tgaggaagtc catgtgccac gacttccgcc gcagctcgcc gtcggtgatc ccctcgagcc
     3481 cggccgcttc ctgaagccgc accgcttcgc gcaccgcctc gtcctcggcg gcggccagcg
     3541 cggccgcgtc gatgcgcccg tcctcgtggt cccgccgccg cgccttgagg gcgggcgacc
     3601 ggagcaggct gcccacgtgg tcggcccgga acggcgggcg cggcgcggtc acgggcggta
     3661 gagcccgcgc ccggccgcga agacgcgggt caggccgagc agcgcgtcgg tgctcggagt
     3721 gccgacgccg acccggccgc cgatctcgct caccgcggtg aggagcgcgt cgatctcgag
     3781 gggcttgccc gcctccacgt cctgcagcat cgaggtcttg aaggccccga gcttgcgggt
     3841 caccgcgttg cgctcctcgc cgctctggcg gatcgggcag ccgatcttct cgccgatcgc
     3901 ggccgcctcc gccatgatcg ccagcgtgaa ccgatcgagg agcggatcgt cgagcagccg
     3961 atcgcaggtg gccccggtca gcgccgacat cgggttcatc gtcatgttgc cccagagctt
     4021 gtaccagacg tcggcctgga tgcgcaggga gacggtggtc tcgaagcccg ccgcctcgag
     4081 ccgctccgcg agcgcgctca cccgggccga ggtcccaccg cccggctcgc cgatgatgag
     4141 cccacgcccg aagccgtggc gcccgagccc gggctccacc accgagcagg tcgcgtgcac
     4201 cacgcagccg atcacgtggc gcaccgggat cgcggccgcc acgcggccgt ccgggtcgac
     4261 cgcgcgcacc gcggcgccct cgtgcgagcc gccgaggccg tggaa
//
LOCUS       DATMCV010000709         5270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_504756, whole genome shotgun sequence.
ACCESSION   DATMCV010000709 DATMCV010000000
VERSION     DATMCV010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504756"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1060)
                     /locus_tag="VNU03_16245"
     CDS             complement(128..1060)
                     /locus_tag="VNU03_16245"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWP76074.1"
                     /translation="MPERPLPTEQEVRALIHDRRNWGRWGKDDQLGAINLITPAKRAA
                     AARLVRTGRSVSLSRPFPKEPGLNNALPAHHYMRTLPRGKGGFAADYYGIFYHGVAST
                     HLDALCHTWDEHAMWNGRDPKKEITFDGATFGSVEHWAEGILTRAVMLDVPRHRGTGS
                     VTEARPVHGWELEDIVRARGLRLEPGDAVCVYSGREAWQAANPERPYGRPFGIGPNER
                     PGLHVSCLEFLRDHDVSVLVWDMLDHLPVGYDIPWAVHAALFAYGVALVDNALLDSLA
                     RACVEEQRDEFMLIVAPLVVVGGTGSPANPLAVF"
     gene            1404..2405
                     /locus_tag="VNU03_16250"
     CDS             1404..2405
                     /locus_tag="VNU03_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWP76075.1"
                     /translation="MTKFIVHCSVAILLILIAATLCPAAEDMGIITGSDKGTYYQFGL
                     DLQKLSKPTGVNLTVHTSKGSIENIFAVYQRPGVQMGIVQSDVLAFVARVQSDPVLQR
                     IAKKTRMVFPLYNEEVHVLGRKGIRDFDDLAGKRVAIGRDGSGTYLTARLLFKLSEVA
                     PSEMVPIDTGEALGELKAGRIDAMFYVAGYPVKLLKDDVTEKDGLELIPITNKSITEF
                     YPRAEIPANIYEWQRTPVNSVAVKAVLVSFDFRRKDCDNVGRFAQTISRQMSWLLQNG
                     HSKWKVVDLNYPLKGWEQYDCVRKYVGTAAPAGTASPAKPAQNENPVFNAIKGLLDE"
     gene            2413..3261
                     /locus_tag="VNU03_16255"
     CDS             2413..3261
                     /locus_tag="VNU03_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76076.1"
                     /translation="MPGSNRLLSLAAAVGSVALLAVVIFMLWPADPSDTATADRDQLD
                     GEAIGWVSKVEANTIHVNSGPFGGGVLPLLVTRNTRIMVGSKEGWFEDIRPGGQVKVV
                     YQIAHGKRLARTVELLVDEGPRRPARPEPRVKSTSGSATGGDATAERVPAKPAVASSP
                     PAPEPKTLDAPPAKPPVAAPPPPSAAPAKPAAPAPPEVRPAARPEPARAQATEPALSR
                     PSSSNGRGNGAADSGARLPDSSRATQPATPVAPAATRPAEPPRASEGEGTDGAAAVDW
                     LLKGRR"
     gene            3310..3813
                     /locus_tag="VNU03_16260"
     CDS             3310..3813
                     /locus_tag="VNU03_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP76077.1"
                     /translation="MSRRARVDPLPHLAAVSAAVRTSGQPEATFRALDAAVAATLGHK
                     LFTCLLHHPAARESERRYTNQPEAYPVGGRKPVPASPWARRLFEERQPYIGYTADDIR
                     QIFFDHALIISLGCASVLNLPVVHDGRTLGVLNLLHEARWYGEDDVPLGQVFAALAVP
                     AFRLLSG"
     gene            complement(3855..>5270)
                     /locus_tag="VNU03_16265"
     CDS             complement(3855..>5270)
                     /locus_tag="VNU03_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76078.1"
                     /translation="RAGHILAFDGRGHRLLRDGVVVVEGDHILAVGPRFDGTVDETVD
                     ARDRVVTPGLISTHAHIGGSPLDRSFIEDRGNPQFWFSGLFEMLPVRSEAQDEEGGRA
                     CVDFSMPELLRGGVTTVMEIGGQGEYVVERAAHYGLRVYMGQAFRSGRWLTRDGKRAE
                     WEWSEEQGREGLRRAVDFHARHDGAHGGLVRCFFAPAQIDTCTPALLKEAKRLADEAR
                     VPYQVHTAQSVVEFNEMVARHGKTPIAWMRELGVLGPNTILGHAIIIGGSSWTNYPAG
                     DVAIMADAGCSVAHAVWVFARRGVAMESFARYRAAGVNMSLGTDTNPQSVIEAMRWAA
                     VCSKIVERNTEATTAAHAFDAATLGGARALGRDDLGRIAPGAKADLVLWRATSWRMTP
                     LRDPIKNIVYNAADEDVDRVYVNGRLVVDAGKVLAADDGAILGALQAAGDRMWPRMAK
                     SDWAGRSADQLSPQTYPDWDA"
BASE COUNT          796 a         1905 c         1816 g          753 t
ORIGIN      
        1 ggtgcccgac gatctcttcg cccagatgcc ggtgatggac cacggcgact tcctgatccc
       61 cgaccggccc ggccacggca tggcgctggc cccggaggcc gagcggaagt accggatcga
      121 ctgacgctca gaacaccgcg agcgggtttg cgggggatcc ggtcccgccc accaccacca
      181 gcggcgcgac gatcagcatg aactcgtcgc gctgctcctc cacgcacgcc cgcgcaagcg
      241 aatcgaggag cgcgttgtcc acgagggcga ccccgtaggc gaagagcgcg gcgtgcaccg
      301 cccaggggat atcgtagccc accggcaggt gatccagcat gtcccacacc agcacgctca
      361 cgtcatggtc gcgcaggaac tcgaggcagg agacgtgcaa gccggggcgc tcgttgggcc
      421 cgatcccgaa gggccggccg taaggccgct cggggttcgc ggcctgccag gcctcgcgac
      481 ccgagtagac gcagaccgcg tcgcccggct cgagacgcag gccgcgggca cgcacgatat
      541 cctccagctc ccagccgtgc accggccgcg cctcggtcac cgaccccgtc ccgcgatggc
      601 gcggcacgtc cagcatcacc gcgcgggtga ggatgccttc cgcccagtgc tcgaccgacc
      661 cgaaggtcgc cccgtcgaag gtgatctcct ttttcggatc gcgcccgttc cacatcgcgt
      721 gctcgtccca cgtgtggcag agggcgtcga gatgggtgga cgccactccg tggtagaaga
      781 tgccgtagta gtcggccgcg aagccgccct tgccgcgcgg cagcgtgcgc atgtagtgat
      841 gcgcggggag cgcattgttg agacccggct ccttcgggaa cgggcggctc agcgagacgc
      901 tccgcccggt ccgtacgagt cgagccgcgg cggcccgctt ggccggcgtg atgaggttga
      961 tcgcgccgag ctgatcgtcc ttcccccacc gtccccagtt gcgacgatcg tggatcagcg
     1021 cgcggacttc ctgctcggtc ggcagcggac gctcgggcat ggtcattcct ccggaagcgg
     1081 gactctacgc cgccgaccgg gcccgcgcaa ggctttgacg cgtggcgtta tcagggttga
     1141 ctcgcccgag cacgtgtggc acatttacgg ccccgcgctt ccctatccgg agcccgggcc
     1201 gacggcgaca cccacgccgg gtcgtcggag gtggtccgag cgggccacgt tgggtaggga
     1261 attgtcctgt agtacggagt gctccaccat gcaaggtcct cgcggttggc ggtaactcac
     1321 cgcgttcatt tgacttcggc gactggtacg gcgcttgccc catggaagcc gtgggggaaa
     1381 ctttcgccta gggggacatg ccagtgacga agttcatcgt tcattgctcg gtagccatcc
     1441 tattgatcct gatcgcagca accctgtgtc cggccgcgga ggacatgggg atcatcaccg
     1501 gcagcgacaa gggcacgtac tatcagttcg gcctcgatct gcagaagctg agcaagccca
     1561 ccggcgtgaa cctgacggtt cacacgtcga agggctcgat cgagaacatc ttcgcggtct
     1621 accagcggcc gggcgtgcag atgggcatcg tccagtccga cgtgctcgcc ttcgtggccc
     1681 gcgtccagtc ggacccggtc ctgcagcgca tcgccaagaa gacgcggatg gtcttcccgc
     1741 tctacaacga ggaagtgcac gtgctcggcc gcaagggcat tcgcgacttc gacgatctgg
     1801 ccggcaagcg ggtggccatc ggccgtgacg gcagcgggac gtacctgacc gcgcgcctgc
     1861 tcttcaagct ctccgaggtc gccccgagcg agatggtgcc gatcgacacc ggcgaggcgc
     1921 tcggcgagct caaggcgggc cgcatcgacg cgatgttcta cgtggcgggc tacccggtga
     1981 agctcctcaa ggacgacgtg accgagaagg acgggctcga gctgatcccg atcaccaaca
     2041 agagcatcac cgagttctac ccgcgcgcgg agatcccggc caacatctac gagtggcagc
     2101 ggaccccggt caacagcgtg gcggtgaagg cggtgctggt ctccttcgac ttccgccgca
     2161 aggactgcga caacgtgggc cgcttcgccc agaccatctc gcggcagatg agctggctgc
     2221 tgcagaacgg gcattcgaag tggaaggtcg tcgacctcaa ctatccgctg aagggctggg
     2281 agcagtacga ctgcgtgcgg aagtacgtcg ggactgccgc gccggccggc accgcgagcc
     2341 cggccaagcc cgcgcagaac gagaacccgg tcttcaacgc catcaagggg ctccttgacg
     2401 aatagacacg tggtgcctgg aagcaaccgt cttctttccc tggcggccgc ggtcgggagc
     2461 gtagcgctcc tggccgtggt catcttcatg ctctggcccg ccgacccatc ggataccgcc
     2521 accgcggacc gagatcagct cgacggggag gcgatcggct gggtctccaa ggtcgaagcc
     2581 aacaccattc acgtcaactc cggtccgttc ggtggcggcg tgctgccgct gctcgtgacc
     2641 cgcaacaccc ggatcatggt cggctccaag gagggctggt tcgaggacat tcgcccgggg
     2701 ggccaggtca aggtcgtgta ccagatcgct cacggcaagc ggctcgcccg caccgtggag
     2761 ctcctggtgg acgaagggcc gcgccgtccg gcccgcccgg agccgcgcgt gaagagcacg
     2821 tccggaagcg cgaccggcgg agacgcgacg gccgagcggg taccggccaa gcccgccgtc
     2881 gcgagcagcc ccccggcccc cgagccgaag acgctcgatg ctccgcccgc gaagccaccg
     2941 gtcgccgccc caccgccccc gtccgccgcc ccggccaagc cggcagcccc cgcgccgccc
     3001 gaggtgcgac cggcggcacg accggagccg gcgcgcgccc aggccaccga gccagctctc
     3061 tcgcggccat cgtcatcgaa cggacgcggg aacggcgcgg cggacagcgg ggcacggctg
     3121 ccggactcct cgcgcgcgac gcagcccgca actccggtcg cgccggccgc gacccggccc
     3181 gccgagccgc cccgagcctc cgagggcgag ggcaccgacg gggcggcggc ggtggactgg
     3241 ctcctgaagg ggcgtcgcta gtccgacccc gggacgtcgc gtgccggccc ggccatgcta
     3301 gggtgcggca tgagccggcg agcccgggtc gatcccctgc cgcacctggc cgcggtgtcc
     3361 gcggcggttc ggacctcggg ccagcccgag gcgaccttcc gggcgctcga cgcggcggtg
     3421 gccgccacgc tcggccacaa gctcttcacg tgcctgctcc atcatccggc ggcgcgcgag
     3481 tcggagcggc gatatacgaa ccagcccgag gcctacccgg tcggcgggcg aaagccggtg
     3541 ccggcgagcc cgtgggcccg gcgactcttc gaggagcgac agccctacat cggctacacc
     3601 gcggacgaca tccgccagat cttcttcgac cacgccctga tcatctcgct cggctgcgcg
     3661 tcggtcctca acctgccggt ggtccacgac ggccgcaccc tcggcgtcct caacctcctg
     3721 cacgaggcgc gctggtacgg agaggacgac gtgccgctcg gccaggtctt cgcggccctc
     3781 gcggtcccgg ccttccggct cctctccggc tgaccgctag gggcgcggcg cgcgcatccg
     3841 gggcggcacg gcgcctaggc gtcccagtcg gggtaggtct gaggcgagag ctgatccgcg
     3901 ctgcgtcccg cccagtccga cttggccatg cgcggccaca tgcggtcgcc cgccgcctgc
     3961 agcgcgccga ggatggcccc gtcgtctgcg gcgaggacct tgcccgcgtc gaccacgagg
     4021 cgcccgttga cgtagacgcg gtcgacgtcc tcgtcggccg cgttgtagac gatgttcttg
     4081 atcgggtcgc gcagcggagt catccgccac gaggtcgccc gccacagcac gaggtcggcc
     4141 ttcgcccccg gcgcgatgcg cccgaggtcg tcgcgcccga gtgcgcgcgc cccgccgagg
     4201 gtggccgcgt cgaaggcgtg cgcggcggtg gtggcctcgg tgttgcgctc cacgatcttc
     4261 gagcacaccg cggcccaacg catggcctcg atcacgctct gggggttggt gtcggtgccg
     4321 agcgacatgt tgaccccggc ggcgcggtag cgcgcgaacg actccatggc caccccccgc
     4381 cgcgcgaaca cccacaccgc gtgggcgacc gagcagccgg cgtccgccat gatcgccacg
     4441 tcaccggccg ggtagttcgt ccaggaggag ccgccgatga tgatggcgtg gcccaggatg
     4501 gtgttggggc cgagcacgcc cagctcgcgc atccaggcga tgggagtctt gccgtggcgg
     4561 gccaccatct cgttgaactc caccaccgac tgcgcggtgt gcacctggta cggcacgcgc
     4621 gcctcgtccg cgagccgctt cgcttctttc aggagcgcgg gggtgcaggt gtcgatctgg
     4681 gctggcgcga agaagcagcg cacgaggcct ccgtgggcgc cgtcgtgccg ggcgtggaag
     4741 tccaccgcgc gccgcaggcc ctcgcgcccc tgctcctcgc tccactccca ctccgcgcgc
     4801 ttgccgtccc gcgtgagcca gcgcccggag cggaaggcct ggcccatgta gacccggagg
     4861 ccgtagtggg ccgcgcgctc caccacgtac tcgccctggc ccccgatctc catcacggtg
     4921 gtgacgccgc cccgcagcag ctcgggcatc gagaagtcca cgcaggcgcg cccgccctcc
     4981 tcgtcctgcg cctcgctgcg caccggcagc atctcgaaga ggcccgagaa ccagaactgg
     5041 ggattccccc ggtcctcgat gaaggagcgg tccagcggtg agccgccgat gtgggcgtgg
     5101 gtcgagatca gtccgggggt gaccacgcgg tcacgcgcgt cgacggtctc gtcgaccgtg
     5161 ccgtcgaagc gcgggccgac cgccaagatg tgatcgccct ccaccaccac gaccccgtcc
     5221 cgcaggaggc ggtggccgcg gccgtcgaag gcgaggatgt ggccggcgcg
//
LOCUS       DATMCV010000710         4061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_105094, whole genome shotgun sequence.
ACCESSION   DATMCV010000710 DATMCV010000000
VERSION     DATMCV010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105094"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VNU03_16270"
     CDS             complement(<1..303)
                     /locus_tag="VNU03_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76079.1"
                     /translation="MTDMWGDLVWAALADLKTRLMLVLPGILAMLTLAAVGLAGAWVS
                     ARVARRLAQAVAFDRRAEAWGIVAALNRAGVRKPPSQVLGLVVFWSVLVLFLPVAID"
     gene            598..750
                     /locus_tag="VNU03_16275"
     CDS             598..750
                     /locus_tag="VNU03_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HWP76080.1"
                     /translation="MSRVLIVILLVLALGAVPAWPHSRDWGYYPSSALTVAGLILLVL
                     VLTGRL"
     gene            785..1288
                     /locus_tag="VNU03_16280"
     CDS             785..1288
                     /locus_tag="VNU03_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HWP76081.1"
                     /translation="MSRASQSVPVRERIAQGFTKVEDVLYVGLGVVLAAGGVALLVNA
                     VIHFVRSLLGGEIGPSVVTVLDQLLLILMIIEILYTVQVSFREHVLVPEPFVLVALIA
                     GVRRILVITAEFGTLTDQGESAFRYAMSELGLLTAMTGVLVTCLVGLRKRDKESGGRA
                     GVMGGAS"
     gene            1285..1749
                     /locus_tag="VNU03_16285"
     CDS             1285..1749
                     /locus_tag="VNU03_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014685076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HWP76082.1"
                     /translation="MKIRQLDDAGGSRTFVLVCDRGDDPVGALTDAAKRYDLRAASLT
                     GIGAFAGVTLGFFDHARRDYRRRVIREQVEVVSLIGNIALDRGEPRVHAHIVVARADG
                     GALGGHLLGGSVEPTLEVTLVEAPATLQRKTDPSTGLALIDLEWEDSRGGGR"
     gene            1977..3356
                     /locus_tag="VNU03_16290"
     CDS             1977..3356
                     /locus_tag="VNU03_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP76083.1"
                     /translation="MKHRILIADDDESGRSGLAALLTIWGYEVQEAVDGKDALERAPA
                     FRPAVVIADMIMPVIDGIGLLKPLAAAVPEAAVILLTAHGSIETAVSAMREGAYDYMT
                     KPVDPRRLRVLVEKAVEKVQVNKEVTVLRRQLKESRGLGPLLGVSPAMQEVYRLIEMA
                     SPSPAPVLILGETGTGKELVARTIHQMSQRAKGPFVAVNCSAIPETLLESELFGHEKG
                     AFTGAMARRPGYFELADGGTIFLDEIAEMSSNLQAKYLRVLQDSQVRRVGGHDEIGVD
                     VRVVAATNKDVVKAVQEGTFREDLYYRVNVLTIPLPPLRRRLEDIALLADAFIAEFNE
                     KYGRQVRSVDGASLKLLNDHTWPGNVRELRNVIERAVVSCEDGLITPECLPLSPLGAP
                     PAERANVVVLPLGTTLDDGERALILRTLQSVNNNKTRAAEILGTTPKTLHNKARRWRE
                     LQLDPEKRA"
BASE COUNT          640 a         1371 c         1471 g          579 t
ORIGIN      
        1 gtcgatggcc accggcaaga agagcacgag gacgctccag aagacgacca gtccgagcac
       61 ctgcgagggc ggcttgcgca cgcccgcgcg gttcagcgcg gccacgatgc cccacgcctc
      121 cgcgcgtcga tcgaaggcta ccgcctgcgc gagccggcgg gcgacgcggg cgctgaccca
      181 cgcaccggcc agcccgaccg cggcgagcgt cagcatcgcc aggattcccg gcagcacgag
      241 catcagccgc gtcttgagat cggcgagagc ggcccacacg agatcgcccc acatatcggt
      301 catgatccgc ccctccaaga gagcaaggtc gacaaaagag caaggtcgac gccaactggt
      361 cgctcacacg gtaccacgcg gcccacgcgc cagaaaggcg cgacaaggcg cgacgggcgg
      421 gtcggcatta ccgggtttct ccggtaacgt ttaccggcgt cccgcgcgcg tcgacaccga
      481 tgcgcccgag cccggcgcgc cgtcgtgcgc ccgttattga gcgcttagcc gcgccgcgca
      541 ggtgctcggt tcgctggcac agcgattgcc gaatagcggg ccggaggcaa cccgcacatg
      601 tcccgcgtgt tgatcgtgat cctgctcgtg ctggcgctcg gcgcggtgcc cgcgtggccg
      661 cacagccggg actggggata ctacccgagc agtgcgctga cggtggcggg cctcatcctg
      721 ctcgtcctcg tgctcaccgg tcgcctgtga gccggtggcc gccgccgccc cgggtcgtcg
      781 cgccgtgagc cgcgccagcc agtccgtccc cgtccgggag cggatcgcgc agggattcac
      841 caaggtcgag gacgtgctct acgtggggct gggggtcgtg ctcgcggcgg ggggcgtggc
      901 cctgctcgtg aacgcggtga tccatttcgt ccggagcctc ctgggcggcg agatcgggcc
      961 gagcgtggtc acggtcctcg accagctgct cctgatcctc atgatcatcg agatcctcta
     1021 caccgtgcag gtctcgttcc gcgagcacgt gctggtgccc gagccgttcg tgctggtggc
     1081 cctgatcgcg ggcgtgcggc gcatcctggt gatcaccgcg gagttcggca ccctgaccga
     1141 ccagggcgag tccgcgttcc gctacgccat gagcgagctc gggctgctca ccgcgatgac
     1201 cggcgtgctc gtgacctgcc tggtgggtct ccggaagcgg gacaaggaaa gcggtggccg
     1261 agcgggcgtg atgggaggag catcgtgaag atccggcagc tggacgatgc gggcgggagc
     1321 cgcaccttcg tgctcgtgtg cgatcggggc gacgacccgg tgggggccct gaccgacgcg
     1381 gccaagcgct acgacctccg ggccgcctcc ctgaccggca tcggcgcgtt cgccggggtg
     1441 accctgggct tcttcgatca cgcccggcgt gactaccgcc gccgggtcat tcgcgagcag
     1501 gtcgaggtgg tatccctgat cggcaacatc gcgctggatc ggggggagcc gcgggtgcac
     1561 gcccacatcg tcgtggcccg ggcggacggt ggggccctgg gcggccacct gctcggcggc
     1621 agcgtggagc cgaccctcga agtcaccctg gtggaggctc cggccaccct tcagcgaaag
     1681 accgatccct ccaccggcct cgccctgatc gatctggagt gggaggactc tcgcggtgga
     1741 gggcggtagt tacacggtaa aatattccct cctccgaggg gccgtccatc gcaagccgcg
     1801 gtaaagtcct ctcggaagct tcgaccacaa atactagtag ttattgagct tactgcgctt
     1861 cggtcggtat ctttttccac aaggaacttt cgaggcacat cgattgcagc cgggtcgcgc
     1921 agtgccgacc agatgactcc atcgaccgac accaatcacc cgagagggga cgaccaatga
     1981 agcatcgcat tctgatcgcc gacgacgacg agtcgggccg cagcgggctg gccgcgctcc
     2041 tgaccatatg gggctacgag gttcaggagg ccgtggacgg caaggatgcg ctcgagcggg
     2101 cgcccgcgtt ccggccggcg gtcgtgatcg cggacatgat catgccggtc atcgacggca
     2161 tcgggctcct gaagcccctc gccgccgcgg tgcccgaggc ggcggtgatc ctgttaaccg
     2221 cccacggctc gatcgagacc gcggtgtccg ccatgcggga gggcgcctac gactacatga
     2281 ccaagccggt ggacccccgc cggctgcgcg tgctcgtcga gaaggcggtc gagaaggtcc
     2341 aggtgaacaa ggaggtcacg gtgctgcgcc ggcagctcaa ggagagccgc gggctcgggc
     2401 cgctcctcgg ggtgagcccc gcgatgcagg aggtctaccg cctgatcgag atggcctcac
     2461 cgagtcccgc ccccgtgctg atcctggggg agaccggcac cggcaaggag ctggtggccc
     2521 gcaccatcca ccagatgtca cagcgggcga agggtccgtt cgtcgcggtg aactgctcgg
     2581 ccattccgga gaccctcctc gagagcgagc tgttcggtca cgagaagggc gcgttcaccg
     2641 gggcgatggc ccgccggccc ggctacttcg agctggccga cgggggcacg atcttcctgg
     2701 acgagatcgc cgagatgagc tcgaacctcc aggcgaagta ccttcgcgtg ctccaagata
     2761 gccaggtgcg gcgcgtcggc ggccacgacg agatcggcgt cgacgtgcgg gtggtggcgg
     2821 ccaccaacaa ggacgtggtg aaggcggtgc aggagggcac gttccgcgag gacctctact
     2881 accgcgtcaa cgtgctgacg atcccgctac ccccgctgag gcggcggctg gaggacatcg
     2941 cgctcctcgc ggacgcgttc atcgccgagt tcaacgagaa gtacggacgg caggtgcgga
     3001 gcgtggacgg ggcgtcgctc aagctcctga acgaccacac ctggccgggc aacgtccgcg
     3061 agctccggaa cgtgatcgag cgagcggtgg tctcgtgcga ggacgggctc atcacgcccg
     3121 agtgcctgcc gctgtcgccg ctcggcgcgc cgccggcgga gcgggcgaac gtggtggtgc
     3181 tgccgctggg aaccacgctg gacgacggcg agcgggcgct catcctgagg acgctgcagt
     3241 cggtgaacaa caacaagacc cgcgccgcgg agatcctcgg caccacgccg aagacgctcc
     3301 acaacaaggc ccgccgctgg cgggagctgc agctggatcc ggagaagcga gcctagaccg
     3361 cgcggcctag cggcggcggg agccgccgct aggctgggcg ccgatccaca ccacccacgg
     3421 ccgcgacgac gaggcatcct gtcggatctc gacgggatag ccctgcttct cgatgctgct
     3481 cgcgaggcga ctcgcggccg cgcgatccgt gaaggcggcc gccggggtcc agtcgcgtgg
     3541 ctcccggggg gcgcggcgcg gctccacggc cgcgcgtggc tccgcggcgg gggccggtac
     3601 agtcgcggcg ctccgcgatg gctccggaac gcggggctcc ggcgcgatgg gcggcgtggt
     3661 cggggccgtg cgcgccgggg gcagtgacgg gggcagtgag gggaccggct cgggcgccac
     3721 cgcggccgct cgctcctcga tcttggactc gagcttgggg cactggaccc tggagggctc
     3781 gaccctgggg gactcgacct tgggctcgtc gggcaccggc ggggtcgacc gagacggatc
     3841 ggtcgtcata cggcccccca agcccccgcg cggatcaccg aggccggtcc gcaagtccag
     3901 gccgctccgc gaatcaccgg ggaccgcctc cgatgacgac gggaacgcct tcggatcgat
     3961 ctcgggagcc ggggaggcga cggacgcact cggcgtccgg gccggcacgg cagagcccac
     4021 gaggtacggt agtccgaaga agatcagcaa gaatgtggga g
//
LOCUS       DATMCV010000711         4415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_441405, whole genome shotgun sequence.
ACCESSION   DATMCV010000711 DATMCV010000000
VERSION     DATMCV010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441405"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2244)
                     /locus_tag="VNU03_16295"
     CDS             complement(<1..2244)
                     /locus_tag="VNU03_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76084.1"
                     /translation="MWTPLNKRSLPVALIALLLIGVIAGSAWAQAPRAERPSYAIGDK
                     WIRSDGAYDLIRIENERYVFASDGGREVHLTRDLGVAKIARGGKTLLELEPPPALTWP
                     LDVGQWGVSWLTLKTLDPQYGQSLVRLSWRVEAYEDVRVPAGTFKAYRIVQVLEPRFL
                     AGSPQSRRIELAFWYAPAVQQIVRAEGSDLTGVAFQAIALDRPGPAPLSVVLIDPKDK
                     GHVATDRVTLTGKVIGGAGPLRIAITLNGAEVAQQQEPGTPGKDVALSMPIKLVEGEN
                     NLIVTATDASGAMRQQGRVLFYDKPGATVAAAAPAPPPSSTPAPPASAPARPAVTPPP
                     GVTPPVIAAPPPVVSAPPTPVPPAAAPPAPPVASAPIPSPPSSSPSAPAPPPPAVASA
                     PPTTPAPTAPLSPRAPVVATPVAPAAPVTAAPATPEPPRSEPRPQPEPARAIPATPTA
                     PAAAPTVVAAIPPVAPAPGFRIVISTPTNQARVDQETVTFAGLVHGGTGVRQVLVTAN
                     GVELWRQDNRSQQPAMAVNLPVKLVEGQNTLVVTAAEIDGTMHQEMRTIYLEKAVPLA
                     VDVRYPEDRSRVTDDASVVAAVARSSKGISRITVALNGAEVHRQDERSPQKSMAVSAP
                     LTLREGPNAIVITALEPDGAARQEIRTVILERPKPTVPAAPPAPAPPPPSTQWAVIIG
                     VGGYESTAVPKLRYPVADADAIYQTLIASGGFKKENILLLTDKTERKPTLRNIKWALG
                     TFLARSAH"
     gene            2434..3444
                     /locus_tag="VNU03_16300"
     CDS             2434..3444
                     /locus_tag="VNU03_16300"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWP76085.1"
                     /translation="MHVIQTARSTRFALAAIALAALGGCAEMQGKEAEEPTRPRVFVA
                     NESSNNVTVVDGETFAVVGTVDARNHATHDLAVSRDGRWLFATNLASGRLSAINTRAM
                     ETVASIATGDRAHVVTLTNDNRQAWVANIGDNTISIVDTTSFRILGTIAVGKGPTGLT
                     FSRDGRFAYVSNQGDKTVEVIDTAAHRVVKAIPVGTNPHFLVLGPDGRIWGTNTGGTD
                     IYVIDPAAQDKIASINVGPSPQQIAFGFKGLQGPNAYVTVAGLNRVVVLSADPANLRI
                     LEQIDVGQRPNGISGNREGTRLFVVHEVSNDLKVIDTGSSAVLATVPVGRKPIRVVFH
                     GN"
     gene            3453..4097
                     /locus_tag="VNU03_16305"
     CDS             3453..4097
                     /locus_tag="VNU03_16305"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HWP76086.1"
                     /translation="MISRVGGLVLAWALGAALVAVAGGPRPAAAQGQPVGTAQNAVGT
                     LVIVRTDGIEHRLQGKGSLPLYEGDVLRTEPASQALIQLRPGTPVALNEKTTIKILSR
                     WDKSVAGSGTVRILRVSRGELWAKAGSGERQLEVETPASVVSGRDAEFDLKVGEDGQS
                     ALTVVQGTADFGTPFGQCAVRGGTSSVGARGAACTAPQAANAAATIGWKQAVSQ"
     gene            complement(4111..4299)
                     /locus_tag="VNU03_16310"
     CDS             complement(4111..4299)
                     /locus_tag="VNU03_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76087.1"
                     /translation="MGDESSFVDLLFLAGALVLILVTSAGIGRRRGGKPAEGQRAAAL
                     GILVWLGLLGFALYLAFG"
BASE COUNT          612 a         1503 c         1686 g          614 t
ORIGIN      
        1 gtgcgccgag cgcgccagga acgtgccgag cgcccacttg atgttgcgca gggtgggctt
       61 gcgctccgtc ttgtcggtga gcaggaggat gttctccttc ttgaagccgc cggaggcgat
      121 cagggtctgg tagatcgcgt cggcgtccgc caccgggtag cgcagcttcg gcaccgcggt
      181 ggactcgtag ccgccgacgc cgatgattac cgcccactgc gtggacggcg gcggaggggc
      241 cggggccggg ggggcggccg ggacggtggg cttcggccgc tccaggatca cggtccggat
      301 ctcctggcgc gcggccccgt ccggctcgag cgcggtgatc acgatcgcgt tcgggccctc
      361 gcgcagggtg aggggagcgc tgaccgccat cgacttctgc gggctgcgct cgtcctgccg
      421 gtgcacctcc gccccgttga gcgcgacggt gatccggctg atccccttgc tcgaccgcgc
      481 caccgcggcc accacgctcg catcgtcggt cacacgcgag cggtcctcgg ggtagcgcac
      541 gtccaccgcc agcggcaccg ccttctccag gtagatggtg cgcatctcct ggtgcatggt
      601 cccgtcgatc tcggccgcgg tcaccaccag ggtgttctga ccctcgacca gcttcaccgg
      661 caggttgacc gccatcgcgg gttgctgcga gcgattgtcc tgccgccaga gctcgacccc
      721 gttggcggtg accagcacct ggcgcacgcc ggtgccgccg tgcaccaggc cggcaaaggt
      781 caccgtctcc tggtccaccc gggcctggtt ggtcggcgtc gagatcacga tccggaatcc
      841 gggagctggg gccaccggtg gaatcgccgc gacgaccgtg ggagccgcgg ccggggcggt
      901 cggcgtggcc ggaatggctc gcgcgggctc cggttgtggc cggggttccg accgtggcgg
      961 ctcgggcgtg gccggcgccg cggtgacggg tgcggcgggc gcgactgggg tcgccacgac
     1021 cggagctcgc ggcgacagcg gggcagtcgg ggccggcgta gtcggcggag cgctcgcgac
     1081 ggccggcggc ggcggggcgg gtgcgctcgg gctcgatgac ggcggtgagg ggatcggcgc
     1141 gctcgcgacc ggcggggccg gcggcgccgc ggctggcggc acgggggtcg gcggcgcgct
     1201 cacgaccgga ggcggagccg cgatcaccgg gggagtaacg ccgggcggag gagtcacggc
     1261 gggccgcgct ggcgccgatg ccggcggggc cggggtcgag ctcggcggcg gggcgggcgc
     1321 cgccgcggcc actgtggcgc cgggcttgtc gtagaagagc acacgtccct gctggcgcat
     1381 ggcgccggag gcgtccgtgg cggtgacgat caggttgttc tcgccctcga cgagcttgat
     1441 cggcatgctg agggccacgt ccttgccggg cgtgcccggc tcctgctgct gggcgacctc
     1501 ggcgccgttc agcgtgatcg cgatgcgaag cggaccggcc ccgccgatca ccttgccggt
     1561 gagcgtgacc cggtcggtgg ccacgtggcc cttgtccttg gggtcgatga ggacgacgct
     1621 cagcggggcc ggaccgggcc ggtcgagcgc gatggcctgg aaggcgaccc cggtcaggtc
     1681 gctgccctcg gcccgcacga tctgctggac cgcgggggcg taccagaacg cgagctcgat
     1741 ccgtcgcgac tgcggcgagc cggcgaggaa gcgcggctcc agcacctgga cgatgcggta
     1801 ggccttgaag gtgccggcgg gcacgcgcac gtcctcgtag gcctcgacac gccagctcag
     1861 gcgcacgagg ctctgcccgt actgcgggtc cagcgtcttg agggtgagcc agctcacgcc
     1921 ccactggccc acgtcgagcg gccaggtgag cgccggcggc ggctcgagct cgagcagggt
     1981 cttgccgccg cgggcgatct tggccacgcc gagatcgcgg gtgaggtgga cttcccgccc
     2041 gccgtcggac gcgaacacgt agcgctcgtt ctcgatccgg atcaggtcgt aggccccgtc
     2101 gctccggatc cacttgtcgc cgatcgcata gctcggccgc tcggccctgg gcgcctgggc
     2161 ccacgccgag ccggcgatca ccccgatgag caggagggcg atgagggcga cgggcaggct
     2221 gcgcttattc agtggtgtcc acatcacgag attcgggtct tctccggtct tattcggccg
     2281 gtgatggcag gcatggcgct ccgggctgcc cttgagatta tgcccatccg gccagggtgt
     2341 caacgaaccg gaaacgggga atatccttga ctcgccgggc ctcctggacc aaggtaaggg
     2401 gcgcttttcc aggagggtcc agggagggag cccatgcacg tgatccagac cgcccgatcg
     2461 acacggttcg cgctcgccgc catcgcgctc gccgccctgg gcggctgcgc cgagatgcag
     2521 ggcaaggagg ccgaggagcc cacccgccca cgcgtgttcg tcgccaacga gagctcgaac
     2581 aacgtgacgg tggtcgacgg cgagaccttc gcggtcgtcg gcacggtgga cgcgcgcaat
     2641 cacgcgacgc acgacctggc cgtctcgcgg gacgggcgct ggctcttcgc gaccaacctg
     2701 gcctcgggcc gtctctccgc gatcaacacc cgggccatgg agacggtcgc ctcgatcgcc
     2761 accggcgatc gggcccacgt ggtcacgctc accaacgaca atcggcaggc gtgggtggcc
     2821 aacatcggcg acaacacgat ctcgatcgtg gacaccacca gcttccgcat cctcggcacc
     2881 atcgcggtgg gcaaggggcc gaccggtctc accttctcgc gcgacggccg cttcgcctac
     2941 gtgagcaacc agggcgacaa gacggtggag gtgatcgaca ctgccgcgca ccgcgtggtg
     3001 aaggcgatcc cggtcgggac gaacccgcac ttcctggtgc tgggccccga cggccggatc
     3061 tggggcacca ataccggcgg caccgacatc tacgtgatcg atcccgccgc ccaggacaag
     3121 atcgcctcca tcaacgtggg gccgtcgccg cagcagatcg ccttcggctt caagggcctg
     3181 caggggccga acgcctacgt cacggtggcg gggctgaacc gggtggtggt gctgagcgcg
     3241 gacccggcga acctgcgcat cctcgagcag atcgacgtcg gccagcggcc caacggcatc
     3301 tcgggcaacc gcgagggcac gcggctgttc gtcgtgcacg aggtctcgaa cgatctcaag
     3361 gtcatcgaca ccggcagcag cgcggtgctg gccacggtgc cggtcgggcg caagccgatc
     3421 cgcgtcgtgt tccacggcaa ctgaggcacg tggtgatctc tcgagtcggc ggtctggtgc
     3481 tggcgtgggc cctcggcgcg gcgctcgtcg cggtggcggg cggtccgcgg cccgcggcgg
     3541 cgcagggcca gccggtgggc acggcgcaga acgcggtcgg caccctggtc atcgtgcgga
     3601 cggacgggat cgagcatcgg ctgcagggga aggggtcgct cccgctctac gagggtgacg
     3661 tgctccgcac cgagcccgcg agccaggccc tgatccagct gcgccccggc accccggtgg
     3721 cgctcaacga gaagaccacg atcaagatcc tctcccgctg ggacaagagc gtggcgggct
     3781 ccgggacggt ccgcatcctg agagtcagcc gcggcgagct ctgggccaag gcgggctcgg
     3841 gcgagcgaca gctcgaggtg gagacgcccg cgtcggtggt atccgggcgc gacgccgagt
     3901 tcgacctgaa ggtcggtgag gacggacaga gcgcgctcac ggtcgtgcag gggacggccg
     3961 acttcggcac cccgttcggg cagtgcgcgg tgcgcggcgg gaccagcagc gtcggggcgc
     4021 gcggggcggc ctgcaccgcg cctcaggccg cgaacgccgc ggccacgatc ggatggaagc
     4081 aggcggtgtc gcagtgacgc gggcggcggg tcagccgaag gccaggtaga gcgcgaagcc
     4141 gagcaggccg agccagacca ggatcccgag cgcggcggcg cgctggccct cggcgggctt
     4201 gcccccgcgg cggcgcccga ttccggcgct cgtgaccagg atcagcacga gcgcgccagc
     4261 caggaagagc agatcgacga agctggactc gtcgcccatc taggcggtcg gaagccggtc
     4321 gacgctcatc gcgccggggt ggcgagcgga gggcgcgcga tctcccggat tgcgtcgatg
     4381 cgtgcgctgc gctcgagggc gtcggcatcg aagac
//
LOCUS       DATMCV010000712         3504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_504870, whole genome shotgun sequence.
ACCESSION   DATMCV010000712 DATMCV010000000
VERSION     DATMCV010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504870"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VNU03_16315"
     CDS             complement(<1..800)
                     /locus_tag="VNU03_16315"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76088.1"
                     /translation="MPHADLVLTGGKIVTLDPRSRTVSALAIRGDRIAALGTDAEIAG
                     LIGPDTRRIDLRGRLAAPGLFDGHAHLDREGLKLKWPSLAAARSIDDILQIVDGLVRR
                     AALGEWIVTMPVGEPPLYEGVPDNLAEKRWPTRHDLDRVAPDHPVYIRAIWGHWRNTL
                     PLVSIANSRALARAGIDRDTRPPAPSIEIQRDARGEPTGVLIEHTYKPLVEHTLMAVA
                     PRFTLEDRVTGLAESMRAYNRCGTTSIFEGHGISAEVLAAYQALRERGG"
     gene            916..2154
                     /locus_tag="VNU03_16320"
     CDS             916..2154
                     /locus_tag="VNU03_16320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP76089.1"
                     /translation="MTEVPGTADVVVVGGGVHGASVAYHLARRKAGRVVLVERKFLAS
                     GPTGRSSALVRRFYAWDFLTRTASASADTFQRWADVIGGPDEPGFRQVGALWLAGADT
                     APHLRENVRRARELGARVDLLAPEDVLRLVPELSVDDVVAGAHEPESGYADPSSTTSA
                     LVNRARALGATIVQYVAVEALLADGNRVTGVRTTQGTISAPAVVVCAGLWADRLLRPL
                     GIEVPVTPQRHQMCFFRRPPEFASHPAILDRPHGAYMRPETGNLTIHGLSTYDDVVDP
                     DSYNEGADPGEILRNAELIAQRFPCMEHGLSMGGYSGVYDVTPDHEPVLGAIPEYAGL
                     FADFGWSGHGFKHAPVIGDIMAELVLEGRAAGWDLAPLRWTRFREADLLPPASGASPP
                     HPKLRPELKSRPELKSRRTA"
     gene            2151..3164
                     /locus_tag="VNU03_16325"
     CDS             2151..3164
                     /locus_tag="VNU03_16325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP76090.1"
                     /translation="MNFGLIVSKQHPPGVSMVDRFREHIDQVRAARDAGFDLIVMGQH
                     FLSTPFQEMQSLPALARLAAEAGTMRVGATVILLPLLNPVDVAEQVATLDVITEGRFI
                     FGVGLGYREEENEAFGIAPKERVGRLVESLEVITRLWSGEEVTHHGRFFHLTRARMML
                     RPVQQPHPPIWFAANNDAAVVRAARLGSAWVINPHAKLAVLERQMEVYRGALREAGRP
                     FPAELPIIKELYVAPDRRTALRECQPFLEAKYKAYASWGQDKALPEGDSFSHEFEELV
                     ADRFIVGDPDDAIREITRYAERLGTNCFIFRVQWPGMEQAKVLRTIKLLADRVIPALR
                     GLK"
     gene            3208..>3504
                     /locus_tag="VNU03_16330"
     CDS             3208..>3504
                     /locus_tag="VNU03_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76091.1"
                     /translation="MVQALAASERSETPKGRRVAALVVALVGVAAYAASLGYDFVWDD
                     TLLIQQSVRLHQWGELPRLLLSQFWSEVGEASHYYRPLITLSFFLDLKVWGLHPF"
BASE COUNT          476 a         1212 c         1288 g          528 t
ORIGIN      
        1 ccgccgcgct cgcggagcgc ctggtaggcc gcgagcacct cggccgagat gccgtggccc
       61 tcgaagatgc tcgtggtgcc gcaccggttg taggcgcgca tggactcggc gaggccggtc
      121 acgcgatcct ccagcgtgaa gcgcggtgcc accgccatca gggtgtgctc caccagcggc
      181 ttgtaggtgt gctcgatgag gacgccggtg ggctcgcctc gggcatcgcg ctggatctcg
      241 atggagggtg cgggcgggcg ggtgtcgcgg tcgatgccgg cgcgggccag agcccgggag
      301 ttggcgatcg agacgagggg cagggtgttc cgccagtgcc cccagatggc gcggatgtag
      361 accggatggt ccggagcgac ccggtccagg tcgtgacggg tgggccagcg cttctcggcc
      421 aggttgtcgg gcacgccctc gtagagcggc ggctcgccga cgggcatggt cacgatccac
      481 tcaccgagcg cggcgcggcg cacgagcccg tcgacgatct ggaggatgtc gtcgatggag
      541 cgggcggccg cgagggacgg ccacttgagc ttgaggccct cgcgatccag gtgggcgtgg
      601 ccgtcgaaga gccccggcgc cgcgaggcgc ccgcgcaggt cgatccggcg ggtgtcgggc
      661 ccgatgagcc ccgcgatctc cgcatcggtg cccagggcgg cgatgcgatc gccgcggatc
      721 gccagggcgg agacggtgcg ggatcgcggg tcgagggtca cgatcttgcc gccggtcagc
      781 acgaggtcgg cgtggggcat ggcgcgtatt caaccatcgc gcccggaccg gggtcaattc
      841 gtcgcgcgcc ggacgagccc acgtcccgag ccttgtcgtg gccggcaggt gcataatgtc
      901 gcccgggagg gcgccatgac ggaagtgccg ggtaccgcgg acgtcgtcgt cgtcggggga
      961 ggcgtgcacg gagcgagcgt cgcgtatcac ctggcgcgcc ggaaggcggg gcgggtggtc
     1021 ctggtcgagc ggaagttcct cgcctcgggg cccaccggcc ggtcctccgc cctggtgcgc
     1081 cgcttctacg cctgggattt tctcacccgc accgcgagcg cctcggccga taccttccag
     1141 cgctgggccg acgtgatcgg cggcccggac gagcccggct tccgccaggt cggagcgctc
     1201 tggctggccg gcgcggacac cgcgccgcac ctgcgcgaga acgtgcggcg ggcccgggag
     1261 ctgggggcgc gggtggatct gctggccccc gaggacgtgc tccgcctggt tccggagctg
     1321 tcggtggacg acgtcgtcgc cggcgctcac gagcccgaat ccggctacgc ggacccatcg
     1381 tcgaccacga gcgcgctcgt gaaccgggcc cgcgcgctgg gcgcgaccat cgtccagtac
     1441 gtggcggtgg aggccctcct cgccgacggc aaccgcgtga ccggcgtccg caccacgcag
     1501 ggcaccatca gcgccccggc cgtcgtcgtc tgcgcggggc tgtgggccga tcgcctgctc
     1561 cgtccgctcg gcatcgaggt gccggtgacg ccgcagcgcc accagatgtg cttcttccgg
     1621 cgcccacccg agttcgcgtc gcaccccgcg atcctcgacc ggccccacgg ggcctacatg
     1681 cgcccggaga ccgggaacct caccatccac ggcctgtcca cgtacgacga cgtggtggat
     1741 cccgactcgt acaacgaagg ggcggatccg ggcgagatcc tgcgcaacgc ggagctgatc
     1801 gcgcagcgct tcccgtgcat ggagcacggg ctctcgatgg gcggctactc gggcgtttac
     1861 gatgtcaccc ccgaccacga gccggtgctc ggggcgatcc cggagtacgc ggggctcttc
     1921 gccgacttcg gctggagcgg ccacgggttc aagcacgcgc ccgtgatcgg cgacatcatg
     1981 gccgagctcg tcctcgaggg ccgcgccgcg ggctgggacc tcgcccccct gcgctggacg
     2041 cgcttccgcg aggccgatct gctcccgccc gcgtcgggcg cctcgccgcc ccacccgaag
     2101 ctacgcccgg agctgaagtc acgcccggag ctgaagtcac ggaggaccgc atgaacttcg
     2161 gcctgatcgt ctccaagcag catccccccg gggtgtcgat ggtcgatcgc ttccgcgaac
     2221 acatcgacca ggtgcgcgcg gcccgcgacg ccggcttcga cctcatcgtg atgggccagc
     2281 acttcctgtc gacgccgttc caggagatgc agagcctgcc cgcgctggcg cggctcgccg
     2341 ccgaggcggg gacgatgcgg gtgggcgcca ccgtgatcct tttgccgttg ctgaacccgg
     2401 tggacgtggc cgagcaggtg gcgaccctcg acgtgatcac cgaggggcgc ttcatcttcg
     2461 gagtcgggct cggctatcgc gaggaggaga acgaggcgtt cggcatcgcg cccaaggagc
     2521 gggtgggccg cctggtcgag agcctggagg tcatcacgcg gctctggtcg ggcgaggagg
     2581 tgacccatca cgggcgcttc ttccatctca cccgggcccg catgatgctg cgccccgtgc
     2641 agcagcccca tccgccgatc tggttcgcgg ccaacaacga cgcggcggtc gtccgcgcgg
     2701 cgcgcctggg ctccgcgtgg gtcatcaacc cgcacgccaa gctcgcggtg ctcgagcgcc
     2761 agatggaagt ttaccgcgga gccctgcgcg aggccggccg cccgttcccg gcggagctgc
     2821 ccatcatcaa ggagctctac gtggcgcccg atcgccgcac cgcgctccgg gagtgccagc
     2881 ccttcctcga ggccaagtac aaggcctacg cctcgtgggg ccaggacaag gcgctgcccg
     2941 agggggacag cttctcccac gagttcgagg agctggtggc ggaccgcttc atcgtcgggg
     3001 acccggacga cgcgatccgg gagatcacgc gctacgcgga gcggctcggc accaactgct
     3061 tcatcttccg ggtccagtgg ccgggcatgg agcaggccaa ggtgctgcgg accatcaagc
     3121 tgctggcgga tcgggtaatc ccggcgctgc gggggttgaa gtagccgaag tcgctgcaga
     3181 atattcagtt cgtgagaatc tggctgtgtg gtgcaggcac tggcggcgtc cgagcggtcc
     3241 gagaccccca agggccggcg tgtcgccgcg ctggtggtgg ccctcgtcgg cgtggcggcc
     3301 tacgcggcga gcctcgggta cgacttcgtg tgggacgaca cgctgctcat ccagcagagc
     3361 gtccgcttgc accagtgggg tgagctgccg cgtctcctcc tctcccagtt ctggtcggag
     3421 gtcggcgagg cgagccacta ctaccggccg ctgatcacgc tgtcgttctt cctcgacctg
     3481 aaagtgtggg gcctgcaccc gttc
//
LOCUS       DATMCV010000713         3454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_974449, whole genome shotgun sequence.
ACCESSION   DATMCV010000713 DATMCV010000000
VERSION     DATMCV010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974449"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VNU03_16335"
     CDS             complement(<1..373)
                     /locus_tag="VNU03_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HWP76092.1"
                     /translation="MPKHVGIVGVSAEGAALCYRALCVEGAELFGRHGHPEVTLHTYS
                     LADYMRHIDADDWHAVGDMVLASVAHLQRSGAELVICPDNTVHQAIDLVRDRSPLPWL
                     HIAEEVAAVAAERRFRRVLVLG"
     gene            complement(389..685)
                     /locus_tag="VNU03_16340"
     CDS             complement(389..685)
                     /locus_tag="VNU03_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76093.1"
                     /translation="MGSKLGVSDEKILALADYATSPLYDEAERTALEYADCMTISGRE
                     VGDELFARLRRLYDDDALVELTATIAWENASSKYNRALRIPSQGLWKRSREPRS"
     gene            complement(704..949)
                     /locus_tag="VNU03_16345"
     CDS             complement(704..949)
                     /locus_tag="VNU03_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76094.1"
                     /translation="MTRIEGVDPRRLTDEYAAKVLEAQAKTWGAPLLNHLTYARRPSI
                     FRGVRGMWTGLESSGLIDHRLVALLNRRVAALNGCEF"
     gene            complement(946..1254)
                     /locus_tag="VNU03_16350"
     CDS             complement(946..1254)
                     /locus_tag="VNU03_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWP76095.1"
                     /translation="MKPLFAVIRARGPAWDHSRPLEGQPEWPAHATFMNGLKAEGFVV
                     LGGPLEGTSEVLLIVRAENEAEVRSKLAADPWSANDLLRVARTAPWTLRLGSLEDTGR
                     "
     gene            complement(1251..1784)
                     /locus_tag="VNU03_16355"
     CDS             complement(1251..1784)
                     /locus_tag="VNU03_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP76096.1"
                     /translation="MTHTWSLRRLHAVDDAQIDGLADVLIDCVEGGASVSFMHPLARE
                     RAVAFWRRVGRGVAAGERALLVAEDARGVCGTVQLVLEQPENQPHRAELSKMLVHRRA
                     RRQGLGAALMRAAEDAARECGKTLLVLDTASVDAERLYERQGWVRVGVIPDFALLPHG
                     GLCGTTVYYRNLEGAGR"
     gene            complement(1787..2386)
                     /locus_tag="VNU03_16360"
     CDS             complement(1787..2386)
                     /locus_tag="VNU03_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP76097.1"
                     /translation="MGDAAPDLNQRIADRVGKLRAAQGLSLDALAAKSGVSRSMISLV
                     ERGESSPTAVVLEKLAAGLGVMLASLFDAPGAAAQASGGPVARRADQPLWRDPASGYL
                     RRNVSPPGVPQPMQIVEVRFPPGGRVAFETGGRDLRVHEQVWVLEGAIDVTVGGERHR
                     LREGDCLAMQLDRPTMFHNPTRKPTRYAVVIASETSSRR"
     gene            complement(2474..2977)
                     /locus_tag="VNU03_16365"
     CDS             complement(2474..2977)
                     /locus_tag="VNU03_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HWP76098.1"
                     /translation="MTRFHLAQANIGRIRAPLEDPIMEGFRTQLDPINALADRSPGFV
                     WRLQTEDGNAMAIRPFADERMAINMSVWESLDALQQFVYRTAHVGPLRDRKQWFEPIE
                     GPILVLWWIPAGHVPTVAEAQERLGHLKEHGPTAHAFTFRTPFPSPDGRSGAVAGLDA
                     EFCDWAS"
     gene            3093..>3454
                     /locus_tag="VNU03_16370"
     CDS             3093..>3454
                     /locus_tag="VNU03_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP76099.1"
                     /translation="MPATIAAERPDTPDALRLIDELQTHLESFYPPESRHGFSVDRLI
                     KEGVAFFVLRVDGAPTGCGGIKLFGREYGELKRMYVRPDFRGRKYGEMLVDHLAEHAR
                     AHGVTLLRLETGIHQQAA"
BASE COUNT          503 a         1302 c         1145 g          504 t
ORIGIN      
        1 tgccgagcac cagaacccgc cggaacctcc gctcggcggc gaccgcggcg acctcctccg
       61 cgatgtgcag ccacggcagc ggcgatcggt cccgcaccag gtcgatggcc tggtgcacgg
      121 tattgtccgg gcagatgacc agctccgcgc cgctcctctg aaggtgcgcc acggacgcga
      181 gcaccatgtc gcccaccgcg tgccaatcgt ccgcatcgat gtggcgcatg tagtccgcga
      241 gcgaatacgt gtgcagcgtg acctccgggt gcccgtgccg accgaagagc tcggcccctt
      301 ccacgcacag cgcccggtag cagagggcgg ccccctccgc gctcaccccg acgatcccga
      361 cgtgcttcgg cacctgggcg cggccggcct aggagcgcgg ctcgcggctc cgcttccaca
      421 gcccctggga cggtatccgc agggcgcggt tgtacttgct ggaagcgttc tcccaggcga
      481 tggtcgcggt cagctccacc agcgcgtcgt cgtcatagag gcggcgaagc cgggcgaaga
      541 gctcgtcccc gacctcgcgg ccgctgatgg tcatgcagtc ggcatactcg agcgcggtcc
      601 gctcggcctc gtcgtagagc ggactggtcg cgtagtcggc cagcgcgagg atcttctcat
      661 cggacacgcc cagcttgctg cccacggcag cgttgatgtc ctgtcagaac tcgcagccgt
      721 tcagcgccgc gacccgccgg ttgagcagcg ccaccagccg gtggtcgatc agcccggagc
      781 tctcgagccc cgtccacatg ccccgcacgc cgcggaagat cgacgggcgc cgcgcgtagg
      841 tcaggtggtt cagcagcggt gcgccccacg tcttggcctg ggcctccagg accttcgccg
      901 cgtactcgtc ggtcaagcgc cgagggtcga cgccttcgat ccgtgtcatc gcccggtgtc
      961 ctccagtgag ccgagccgga gcgtccacgg agccgtccgc gccacgcgca gcagatcgtt
     1021 cgcgctccac gggtccgcgg ccagcttcga gcggacctcc gcttcgttct ctgcccgcac
     1081 gatcagcaag acctccgacg tcccctcgag cggtccgccc agcaccacga acccctcggc
     1141 cttgagcccg ttcatgaacg tggcgtgcgc cggccactcc ggctgtccct ccagcgggcg
     1201 cgaatgatcc cacgccggtc cccgcgcccg gatgaccgca aagagcggct tcaccggccg
     1261 gccccctcga ggttccgata gtacaccgtc gtgccgcaga gcccgccgtg cggcagcagc
     1321 gcgaaatcgg ggatcacccc cacccgcacc cagccctgcc gctcgtacag gcgctcggcg
     1381 tcgacgctgg cggtgtcgag caccagcagc gtcttgccgc actcgcgcgc ggcgtcctcc
     1441 gcggcgcgca tcagcgccgc gcccagcccc tggcgccggg cccggcggtg caccagcatc
     1501 ttcgacagct cggcgcggtg cggctggttc tcgggctgct cgagcacgag ctgcacggtg
     1561 ccgcacacgc cccgcgcgtc ctccgcgacc agcagcgcgc gctcgcccgc ggccacaccc
     1621 cgccccaccc ggcgccagaa cgccaccgcg cgctcgcggg ccagcgggtg catgaagctc
     1681 accgaggcgc cgccctcgac gcagtcgatc aggacgtcgg cgaggccgtc gatctgcgcg
     1741 tcgtcgaccg cgtgcagccg tcgcagggac caggtgtgcg tcatgctcat cgcctcgaag
     1801 aagtctccga cgcgatcacc accgcgtagc gcgtgggctt ccgcgtcggg ttgtggaaca
     1861 tggtcgggcg gtcgagctgc atggccaggc aatctccttc gcgcaggcgg tggcgctccc
     1921 cgcccaccgt gacgtcgatc gcgccttcga gcacccagac ctgctcgtgc acgcgcaggt
     1981 cgcgcccgcc ggtctcgaac gcgacgcgcc ccccgggggg gaagcgcacc tcgacgatct
     2041 gcatgggctg cggcacaccg ggcggcgaga cgttgcggcg caggtacccg gaggcaggat
     2101 ctcgccacag cggctggtcg gcccgccgcg ccaccggtcc gccggacgcc tgggccgcgg
     2161 cgcccggggc atcgaacagc gaggccagca tcacccccag gccggcggcg agcttctcca
     2221 gcaccaccgc ggtggggctg ctctcgcccc gctcgacgag cgagatcatc gagcggctca
     2281 ccccgctctt ggcggccagg gcgtcgagcg agaggccctg cgcggcacgc agcttgccca
     2341 cgcgatcggc gatgcgctgg ttcaggtcgg gggctgcatc tcccatggtg gacacagtgt
     2401 ccaccatcct ggatacggcg tcaaggccgg agctgcagcc cgtgaaatcc cgccgttcct
     2461 ctcgaccggg ccctcagctg gcccagtcgc agaactccgc atccaggccc gcgaccgcac
     2521 cagaccgtcc atccggggat ggaaacggcg tcctgaatgt gaaggcatga gcggtcggcc
     2581 cgtgctcctt cagatgcccg aggcgctcct gtgcctcggc gaccgttgga acgtgcccgg
     2641 ccgggatcca ccacaggacg aggatggggc cctcgatggg ctcgaaccac tgcttccggt
     2701 cacgaagcgg cccgacgtgc gcggtccggt agacgaactg ctggagcgca tccagggact
     2761 cccataccga catgttgatg gccatgcgct cgtcggcgaa cggccggatc gccatggcat
     2821 tgccgtcctc ggtctggagt cgccacacga atcccgggct gcggtcagcg agcgcgttga
     2881 tggggtccag ctgggtccgg aagccctcca tgatcgggtc ctcgagcggc gcccgaattc
     2941 gcccgatgtt cgcctgtgcc agatggaaac gcgtcatgcc cgatgcctcc ctcgattgcc
     3001 tatcgtggct cgaccagcgc cgcgagcctc gcgaacgcct ccctcccccg tggcgccttg
     3061 acggtcctgg ctcggcgagg caggatcccg ccatgcccgc caccatcgcg gccgagcgtc
     3121 ccgacacccc ggacgctctg cgtctgatcg acgagctgca aacccatctg gagtcgttct
     3181 acccgccgga gagccggcac ggattcagcg tggatcgatt gatcaaggaa ggggtggcgt
     3241 tcttcgtgct gcgggtggac ggcgcgccca cgggctgcgg cgggatcaag ctctttgggc
     3301 gcgagtacgg agagctgaag cgcatgtatg tccggccgga cttccgcggc cgcaagtacg
     3361 gcgagatgct cgtcgatcac ctcgccgagc acgcccgcgc gcacggcgtc acgctcctgc
     3421 gcctcgagac cggcatccac cagcaggccg cgat
//
LOCUS       DATMCV010000714         6390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_572162, whole genome shotgun sequence.
ACCESSION   DATMCV010000714 DATMCV010000000
VERSION     DATMCV010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572162"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2385)
                     /locus_tag="VNU03_16375"
     CDS             complement(<1..2385)
                     /locus_tag="VNU03_16375"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP76100.1"
                     /translation="MGPLRPHDTPDLHEHGALRARLRRLEEDNARLLDRERTRLQETR
                     ALAGIGRLLSERLEPDVVGLRIAESLRSLLAGGSAVVYRLDADSRSLHALAVSRAADP
                     SSGWRQVREPSSGAVGLAIRERRTIATPDVLADPRIQTGADLRGHLEQAPDRAILAVP
                     LLTQDRLIGVLAVRNATGSVFDARAVQMAEALADQAALTLENARLFAEEEHRRREAEI
                     LAELARTIGGTLDLETVLRRVTEAAQELCRSDGAVIGLRVPGAESVRLRYWTANWYDT
                     LADTCIEPRRGLGGLVLAEGRPMRTSDYLRDPRIGEGYQERIQRLGIHAQMAVPVLID
                     DRVEGLLYVDNRSARAFTDQEEAILARLAAQAALAIRNAQLFADEQVARGTAERLVRA
                     LRESQERFQFVSRATNDAVWDWDLVSGGLWWNEGIQTLFGYTEEQIGPDITWWYELVH
                     PEDRERVVTDIHAAIERGDASWSAEYRCRRADGSYAHVYDRGYVLRDAEGRGIRMIGA
                     MMDVTQRKQLEDELRQAQKMEAVGRLAGGVAHDFNNLLTVITGRSAILLGRLKADDPL
                     RRSVEQIQKTADRAAGLTRQLLAFSRKQVLQRKDLDLNAMVEEVSGMLRRLIGEDVEL
                     LLTLGPKAGHVNADPGQLEQVLMNLAVNSRDAMPRGGTLGVETDCVQVEAAPPDRPEA
                     LPPGPYAVLRVMDTGVGMDAATQARIFEPFFTTKEPGKGTGLGLSMVHGVVRQHGGAV
                     QVRSVVGGGTTFEIYLPQVATPAEPARIAETVGRETAWRETILLAEDEDEVRALT"
     gene            2578..3924
                     /locus_tag="VNU03_16380"
     CDS             2578..3924
                     /locus_tag="VNU03_16380"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWP76101.1"
                     /translation="MTTDAQAIRHLWTHKTQDHPFLSDQELVIDRGEGVWIWTEQGKK
                     LLDGFAGLAVVNVGHGRREIAEAIAEQTVRLAYYPTTRQFSNRPAADLAAKLATLAPG
                     DLRYTMFAVSGSEANERSMQIARQYWLAAGKAGKHKVISLQGGYHGATMGTFAVCGLP
                     HLAQPYQPLEVPGFAKVAPPHPFRDLGTGTEAELIARRIGELREAILREGPDTVSAVI
                     MEPVLSSGGFIMPPIGWLRAVRALCDELDVLMIADEVITGFGRTGRWFAVEHDQVVPD
                     LMSVAKGITSGYIPLSASIARGRLADAFSDTTTQENVHPNTYAAHPVACAAALANLKI
                     MEQDRLVENAAKMGTRLLDGLRNAVGKHPIVGEVRGRGLLVCVDFVEPDGSGRPLDAK
                     HVAELDRKAWDRGAVIYARGTVLRLAPPLCITPAEVDELVAIVADCVDSLQRDVAR"
     gene            complement(3939..5282)
                     /locus_tag="VNU03_16385"
     CDS             complement(3939..5282)
                     /locus_tag="VNU03_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP76102.1"
                     /translation="MRDEQTIEAEATDLLQTLIRNRCVNDGTVASGQETRSVAALETY
                     FNGAGVACERYTSEPGRESLIARIEGSDPKAPRLLLMGHTDVVPVSPDGWKRDPFGGE
                     LVDGVVWGRGAIDMLNLTSTMAVATRRLAKDGFRPRGTLIYLAVADEEAGGNLGAGHL
                     VDRRPEMVKADYVITESGGVPIPTRDGHVLGVTVGEKGANWRHLTVAGTPGHGSRPFR
                     TDNALVTAAEVVRRVAAYQPKARILDVWREYVKAMDLGPELTKGLTDPARVLETVSGM
                     ENLALAREAHACTHTTFSPNIIHGGVKTNVIPDRVDIDVDIRALPGVTTEDVDAMLRE
                     ALGDLAPRVTIKQGRGQQGSLSSHDTPLVAAMERVTSRLMPGSRVVPKMTTGGTDAKF
                     FRWQGVPAYGFGLQSLRIPYTDYPLMFHGHNERVDTESLKLSTMMWETLAREFLA"
     gene            complement(5293..6171)
                     /gene="nudC"
                     /locus_tag="VNU03_16390"
     CDS             complement(5293..6171)
                     /gene="nudC"
                     /locus_tag="VNU03_16390"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HWP76103.1"
                     /translation="MTPLSVYLPFNMECLRGEFEPAKRGGGPPAGVGRWLVVQDQKLL
                     VVPEGDGFRLPAGESPEAPPRGLDGLGPRHWLGTWGGAPCWVAARPKDAPVPEGLAAE
                     TLVPMQGTKLPDDLLTVGGMAMQLLYWESTSGHCPRCGAPAGRLEGEWGKRCEGCRYE
                     HYPHLHPAVIVLVRDGNRVLLARKAIWAPGRYALVAGFVDNGESLEGCVAREVKEEVG
                     VDVKDIQYVGSQNWPFPSQIMIGFVASYAGGEITIDREELEDARWFPSDAMPVGPARH
                     SIAGWIIRNFAANGGM"
BASE COUNT          843 a         2303 c         2283 g          961 t
ORIGIN      
        1 ggtgagcgcg cgcacctcgt cctcgtcctc cgccagcaag atggtctcgc gccaggcggt
       61 ctcgcggccc acggtctccg cgatccgggc gggctcggcg ggagtagcca cctgggggag
      121 gtagatctcg aaggtggtgc cgccgcccac cacgctccgc acctgcacgg cgcccccgtg
      181 ctggcgcacc accccgtgca ccatcgagag cccgagcccg gtgcccttgc cgggctcctt
      241 ggtggtgaag aatggttcga agatgcgcgc ctgcgtggcc gcgtccatcc ccacgccggt
      301 gtccatgact cgcaggaccg cgtacgggcc gggcggcagg gcctccggcc ggtccggcgg
      361 ggccgcctcg acctggacgc aatcggtctc gacgcccagg gtgccgccgc ggggcatggc
      421 atcgcgcgag ttcacggcca ggttcatcag cacttgctcg agctggcccg ggtcggcgtt
      481 gacgtgcccg gccttcggtc ccagggtgag gagcagctcg acgtcctcgc cgatgagccg
      541 ccgcagcatc ccgctgacct cctccaccat cgcgttgagg tccaggtcct tccgctgcag
      601 cacctgcttg cggctgaagg ccaggagctg gcgggtgagg ccggcggccc gatcggcggt
      661 cttctggatc tgctccacgc tgcggcggag cggatcgtcg gccttcagcc ggccgaggag
      721 gatcgcgctg cgcccggtga tcacggtgag gaggttgttg aagtcgtgag ccactccgcc
      781 cgcgaggcgc cccaccgcct ccatcttctg ggcctgccgc agctcgtcct cgagctgctt
      841 gcgctgggtg acgtccatca tggcgccgat catgcggatg cctcggccct ccgcgtcgcg
      901 cagcacgtag ccgcggtcgt agacgtgggc gtacgagccg tcggcgcgcc ggcagcggta
      961 ctccgcgctc cagctcgcgt cgccccgctc gatggcggcg tggatgtcgg tgaccacccg
     1021 ctcgcgatcc tccgggtgga ccagctcgta ccaccaggtg atgtccgggc cgatctgctc
     1081 ctcggtgtag ccgaagaggg tctggatgcc ctcgttccac cagagcccgc cgctgaccag
     1141 gtcccagtcc cacaccgcgt cgttggtggc gcgcgagacg aactggaagc gctcctgact
     1201 ctcgcggagg gcgcgcacca gccgctcggc ggtgccgcgc gcgacctgct cgtccgcgaa
     1261 gagctgggcg ttgcggatgg cgagcgcggc ctgggcggcc aggcgcgcca ggatggcctc
     1321 ctcctggtcg gtgaaggcgc gggccgagcg gttgtctacg tagagcagac cctcgacgcg
     1381 atcgtcgatg agcaccggga ccgccatctg ggcgtggatg ccgaggcgct ggatgcgctc
     1441 ctggtagccc tcgccgatcc ggggatcgcg gaggtagtcg ctcgtgcgca tcgggcggcc
     1501 ctccgcgagg acgaggccgc ccaggccccg gcggggctcg atgcaggtgt cggccagcgt
     1561 gtcgtaccag ttggcggtcc agtagcggag gcgcacgctc tccgcgccgg gcacccgcag
     1621 gccgatcacc gctccgtcgc tgcggcagag ctcctgggcc gcctcggtca cgcgtcgaag
     1681 caccgtctcg aggtcgaggg tgccgccgat ggtgcgggcc agctcggcga ggatctccgc
     1741 ctcccggcgc cggtgctcct cctcggcgaa gagccgcgcg ttctccaggg tgagcgcggc
     1801 ctggtcggcg agcgcctccg ccatctgcac cgcgcgcgcg tcgaagacgc tgccggtggc
     1861 gtttcgcacc gcgagcacgc cgatgaggcg atcctgggtc agcaggggga ccgcgaggat
     1921 cgcgcggtcg ggcgcctgct ccaggtggcc gcggaggtcc gcgccggtct ggatgcgcgg
     1981 gtccgcgagc acgtccgggg tggcgatggt ccgccgctcg cggatcgcga gcccgaccgc
     2041 gccggagctt ggctcgcgca cctggcgcca gccgctcgac ggatcggccg cgcgggacac
     2101 cgccagcgcg tggaggctcc gcgagtccgc gtcgaggcga tagaccaccg cggagccgcc
     2161 cgcgaggagc gagcgcaggc tctccgcgat gcggaggccc accacgtcgg gctcgaggcg
     2221 ctcggagagc agccggccga tgccggcgag cgcccgcgtc tcctggaggc gcgtacgctc
     2281 ccggtccagg aggcgcgcgt tgtcctcctc gaggcgccgc agtctggcgc gcagcgcgcc
     2341 gtgctcgtga aggtccgggg tgtcgtgagg tcgcagcggt cccatcgcgt gtcggtggtg
     2401 gagcaaggac cacgccacgg gtaaatggct gattgtcttg tcgttccagg ccgtgccggc
     2461 acggaaagtg tcagccggcg tcgcaaaatg agcacccggg gacgggccgg gtgccacggt
     2521 gctaccatgg cgactcactg agggcgagcc gctcaacctt tcccgggaga tccgtccatg
     2581 accaccgacg cgcaggccat ccgccacctc tggacccaca agacgcagga ccatccgttc
     2641 ctgtccgatc aggagttggt catcgaccgg ggcgagggag tgtggatctg gaccgagcag
     2701 gggaagaagc tgctcgacgg cttcgcgggc ctcgcggtgg tcaacgtggg ccacggccgg
     2761 cgcgagatcg cggaggccat cgcggagcag acggtgcggc tggcctacta cccgaccacg
     2821 cggcagttct ccaaccggcc ggccgcggac ctggccgcga agctcgcgac cctcgcgccg
     2881 ggcgacctgc gctacacgat gttcgcggtg agcgggtcgg aggccaacga gcgctcgatg
     2941 cagatcgcgc ggcagtactg gctggccgcc ggcaaggcgg gcaagcacaa ggtcatctca
     3001 ctgcagggtg gctaccacgg tgccacgatg gggacgttcg cggtctgcgg gctgccccac
     3061 ctggcccagc cctaccagcc gctcgaggtg ccgggcttcg cgaaggtggc gccgccccat
     3121 ccgttccgcg atctgggtac cggcaccgag gccgagctga tcgcgcgccg catcggggag
     3181 ctccgcgaag ccattctgcg cgaaggcccg gacacggtct ccgcggtgat catggagccg
     3241 gtcctctcgt cgggcggctt catcatgccg cccatcggct ggctgcgcgc ggtgcgcgcg
     3301 ctctgcgacg agctcgacgt gctgatgatc gcggacgagg tcatcaccgg cttcgggcgc
     3361 accggacggt ggttcgcggt cgagcacgac caggtcgtgc cggatctcat gtcggtggcc
     3421 aagggcatca cctcgggcta catcccgctc tcggccagca tcgcgcgcgg gcggctcgcc
     3481 gacgcgttct ccgacaccac cacccaggag aacgtgcacc ccaacaccta cgccgcgcac
     3541 ccggtggcgt gtgcggccgc gctcgcgaat ctgaagatca tggagcagga ccggctggtg
     3601 gagaacgcgg cgaagatggg gacgcgcctg ctcgacgggc ttcgcaacgc ggtgggcaag
     3661 caccccatcg tgggcgaggt gcgcgggcgc ggcctgctgg tctgcgtgga cttcgtggag
     3721 cccgacggct ccggccggcc gctcgacgcc aagcacgtgg ccgagctcga ccgcaaggcg
     3781 tgggaccgcg gggccgtcat ctacgcgcgc ggcaccgtgc tccgcctcgc cccgccgctc
     3841 tgcatcaccc ccgcggaggt cgacgagctg gtggccatcg tcgccgactg tgtcgactcc
     3901 ctccagcgcg atgtcgcgag gtagccgcgg acgtccgtct aggcgaggaa ctcgcgggcg
     3961 agggtctccc acatcatggt ggagagcttg aggctctcgg tgtccacccg ctcgttgtgg
     4021 ccgtggaaca tgagcgggta gtcggtgtag gggatgcgga gcgactgcag cccgaagccg
     4081 taggcgggga cgccctgcca gcggaagaac ttggcgtcgg tgccgccggt ggtcatcttg
     4141 ggcaccaccc ggctccccgg catcagccgc gaggtcaccc gctccatcgc ggccaccagc
     4201 ggggtgtcgt ggctgctcag cgagccctgc tggccgcggc cctgcttgat ggtcacccgc
     4261 ggggcgaggt cgccgagcgc ctcccgcagc atcgcgtcca cgtcctcggt ggtcaccccg
     4321 ggcagcgcgc ggatgtccac gtcgatgtcg acgcgatccg ggatcacgtt ggtcttcacg
     4381 ccgccgtgga tgatgttggg cgagaaggtg gtgtgggtgc aggcgtgcgc ctcgcgggcc
     4441 agcgccaggt tctccatgcc cgagaccgtc tccagcaccc gcgcggggtc ggtgaggccc
     4501 ttggtcaatt cggggccgag gtccatggcc ttcacgtact cgcgccacac gtcgaggatc
     4561 cttgccttcg gctggtaggc cgccacgcgc cgcacgacct cggccgcggt caccagcgcg
     4621 ttgtcggtgc ggaaggggcg cgagccgtgg cccggcgtcc ccgccaccgt caggtgacgc
     4681 cagttggcgc ccttctcgcc gacggtgacg cccagcacgt gaccgtcgcg ggtggggatg
     4741 ggcacgccgc cgctctcggt gatcacgtag tccgccttca ccatctcggg gcgccgatcc
     4801 accaggtggc ccgcgcccag gttgcctccc gcttcctcgt ccgccaccgc cagatagatg
     4861 agagtgccgc gcgggcggaa gccgtccttg gcgaggcgcc gggtggccac cgccatggtg
     4921 gaggtgaggt tcagcatgtc gatggcgccg cggccccaca ccactccgtc caccagctcg
     4981 ccgccgaagg gatcgcgctt ccagccgtcc gggctcaccg gcaccacgtc ggtgtgaccc
     5041 atcagcagca gccgcggggc cttggggtcg gacccctcga tgcgcgcgat gaggctctcg
     5101 cggccgggct ccgaggtgta gcgctcgcag gccacgcccg cgccgttgaa gtacgtctcg
     5161 agcgcggcca cgctgcgcgt ctcctggccg gaggccaccg tgccgtcgtt gacgcagcgg
     5221 ttccggatca gggtttgcaa caggtcggtc gcttccgctt cgatggtctg ctcgtctcgc
     5281 atgatctccc tcttacatgc cgccgttggc ggcgaagttg cggatgatcc agcccgcgat
     5341 ggagtgccgc gcggggccga ccggcatcgc gtcgctcggg aaccagcgcg cgtcctccag
     5401 ctcctcgcgg tcgatggtga tctcgccgcc cgcgtagctc gccacgaagc cgatcatgat
     5461 ctggctcggg aacggccagt tctggctccc gacgtactgg atgtccttga cgtcgactcc
     5521 cacttcctcc ttcacctcgc gcgccacgca gccctcgagc gactcgccgt tgtcgacgaa
     5581 gccggccacc agcgcgtagc ggcccggggc ccagatcgcc ttgcgcgcga gcagcacccg
     5641 gttgccgtcg cgcaccagca cgatgaccgc ggggtggagg tgcgggtagt gctcgtagcg
     5701 gcagccttca cagcgcttgc cccactcgcc ctcgagccgg ccggcgggcg cgccgcagcg
     5761 cgggcagtgc ccgctggtcg actcccagta caggagctgc atggccatgc cacccaccgt
     5821 gagcagatcg tcgggtagct tcgtgccctg catcggcacg agcgtctccg cggcgagtcc
     5881 ctcgggcacc ggcgcgtcct tcgggcgcgc ggccacccag cacggcgcgc cgccccacgt
     5941 gccgagccag tgacgcgggc ccagcccgtc cagcccgcgc ggcggggcct ccgggctctc
     6001 gcccgcgggc aggcggaatc cgtcgccctc cggcaccacg aggagcttct gatcctggac
     6061 cacgagccac cgtccgactc cggcgggcgg cccaccgccc cgcttggcgg gttcgaactc
     6121 gccacgcagg cactccatat tgaagggcaa gtagacggac agcggtgtca tcagcgtgcc
     6181 gagtctacca cggggaaaac aggttgcgct cccgcgccgg ctgggctacg actagctcct
     6241 catgagcgac ctccgcatga gcgacctctg gcaccccgtg ctatcctccc cgccgtgccc
     6301 gacctcctag cgtccctcgc tgtctcggtc gccgcgcgcc tggtcgcgcg ccgcgagacg
     6361 gtggcggtct ccgagtcgtc gaccggcggc
//
LOCUS       DATMCV010000715         6521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_702976, whole genome shotgun sequence.
ACCESSION   DATMCV010000715 DATMCV010000000
VERSION     DATMCV010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702976"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..614
                     /gene="queF"
                     /locus_tag="VNU03_16395"
     CDS             249..614
                     /gene="queF"
                     /locus_tag="VNU03_16395"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWP76104.1"
                     /translation="MPSQPSKTLEVVPNPHPDRDYEVSLEIPEFTCICPRTGQPDFAT
                     IRVRYVPDQRLLELKAIKLYIWSYRNEGAFHEDVTNRILNDFVAAAAPRWIEVVGDFT
                     VRGGIKTVVRATHGKRPEL"
     gene            complement(611..1477)
                     /locus_tag="VNU03_16400"
     CDS             complement(611..1477)
                     /locus_tag="VNU03_16400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWP76105.1"
                     /translation="MWLLLALVAALCQVLRNTVMKRLGHSLDEYINVWGRFTFLLPFA
                     GAFVLWKGVPVIKPGFYLACVCFAVCQTISTMSLSKALKLSEISMVTALWKVSLLVLV
                     VLGYFTLKERPTLLGILGILISMSGVYLLNVNRAHIAWWAPLAVLFTDRGLRYTLLAA
                     LFFAPSVVTIKWAMQCSDQYMGTLGGYLASSLLVTPIVLVTSRRHFVEIPRYWKAFVS
                     FGLFASLTTVSQGTAYLMTLSSYVEAVKQVEILFAMVIGTIMFHEKQKVRDIAPGALV
                     MLAGVVLLSLAG"
     gene            complement(1488..2378)
                     /locus_tag="VNU03_16405"
     CDS             complement(1488..2378)
                     /locus_tag="VNU03_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP76106.1"
                     /translation="MATVRTPTLEIGYEEHGPATGPPIVLLHGFPDDVRAWDGATGPL
                     AAQGCRVLVPYLRGYGPTRFLDPGEPRMAQQAAIGQDLLDFMDALGLERVSLAGYDWG
                     GRAACLAAILAPARVRALVTIGGYNVQNTLAPPRPASAEDERGYWYQWYFNTERGRLG
                     LEQNRREICRLLWRDWSPGWRFDDATFERTAVAFDNPDFVPVVIHSYRHRHRNAPGDP
                     RFDAVERRLAERPRIEVPAVILHGGDDGVDRPQKSERHPDLFPAGTERRVVPDAGHFL
                     PREQPGAVVEALLSLLARTR"
     gene            complement(2397..2990)
                     /gene="leuD"
                     /locus_tag="VNU03_16410"
     CDS             complement(2397..2990)
                     /gene="leuD"
                     /locus_tag="VNU03_16410"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015138305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWP76107.1"
                     /translation="MSESARRQVAGRAIPVPGNDIDTDRIIPARFLKTVTNEGLGLHA
                     FEDARKHDPEHPFNQKAYEGAAVLVAGINFGCGSSREHAPESLKQWGIKAIVGASFGE
                     IFFGNCTMLGIPCVRIDNEDVLWLQKAVGRDPKQPVTVDVEKQEVRVGERVIKGVIPD
                     GARNQLVSGAWNPTGVLLEAGDAIEATAKRLPYVSGF"
     gene            complement(2990..4390)
                     /gene="leuC"
                     /locus_tag="VNU03_16415"
     CDS             complement(2990..4390)
                     /gene="leuC"
                     /locus_tag="VNU03_16415"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWP76108.1"
                     /translation="MPSSLLDKVWDAHTVRTLPSGSTQLLIGLHLIHEVTSPQAFQAL
                     RELKVPVRMPARTFATLDHIIPTTAQQRPFADEVAEELTVHLTRNAQQSGVPFFGLDS
                     GKQGIVHVIGPELGLTQPGMTIACGDSHTSTHGAVGAIAFGIGTSQVRDVLATQCLAM
                     AKPKLRQVRVDGALSKGVYAKDVILAIIRTLGVNGGVGYAYEYAGPVVDRMTMEERLT
                     LCNMSIEGGARFGYVNPDQTTYDYLRGRQYAPQGAAFDRAVSWWQGMASDPDARYDDV
                     KRLDGSAIPPMVTWGINPGQSVGVGERLPNPETAPEQERATYREGLAHMGFEAGQPIA
                     GAKIDVVFIGSCTNSRISDLRVAAAVAKGNRVAKGVRAIVVPGSQQVAKVAEAEGLHE
                     VFREAGFEWRNAGCSMCLGMNDDKLRGREMSASTSNRNFIGRQGSPTGRTLLMSPAMA
                     VAAALHGAVTDVREML"
     gene            4477..5397
                     /locus_tag="VNU03_16420"
     CDS             4477..5397
                     /locus_tag="VNU03_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWP76109.1"
                     /translation="MIDAALLPALLSLLAVARSGSVGAAARLQHRTSSAISQQIRKLE
                     RHLDVKLLERAGRGVRLTAAGEAALPGILQLGTETEALFAQLAELSGRPPAILRVAVS
                     DYLGRDLLVPVLHELLEEQPRLRFEIATTHSRESVRLVGLGEVDCAVVTGQEAPRGIE
                     TARLFDQAFVWVGPRRARRRAPLAERLAREPLLRLGAGSQGRRLLEDYLAREHIQPVS
                     TIDVPSVSLLLTYVSGGLGVGLAPALALAEGGSGRRAYDWERAAVPALTVTLVHRPGF
                     RRSSAAERFATRLAAEGRRAGARLRRMGLG"
     gene            complement(5394..>6521)
                     /locus_tag="VNU03_16425"
     CDS             complement(5394..>6521)
                     /locus_tag="VNU03_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWP76110.1"
                     /translation="IFQRGAVDGLSMVVPHGDPDYYGVRGSIAIARPAPGVAEATVDL
                     DAFFGLHPSLAPLKPLWDDRRLAVVHACGSTDTTRSHFDAQDYMESGTPGVKSTPDGW
                     LARGLAGMPAREPASPFRAVSLGAALPRILRGDVGAVAMSSVSDFDVKEGGAAMAGRP
                     GARQGFESMYEKGVRDLLYGTGRETFEAVKMLKAAAPQRLGPANGAVYPRGRFGDSLR
                     QIAQLIRADVGLEVAFADVGGWDTHAGQGNERGQLAARLREFGEGLAALDRDLGARMS
                     DVVVLTMSEFGRTVRENGNRGTDHGHATAMLALGGGVRGGRVYGRWPGLRREQLFEGR
                     DLAVTTDFRALFTEVAVRHLGAPAGPLFPGFQASALPGLLA"
BASE COUNT          914 a         2387 c         2313 g          907 t
ORIGIN      
        1 agcgcgctcg ccacctgctc gctcaccacc ccgctcgccg gcgtgctcat cgccgccgcg
       61 gtaaccggcg atcgcctgag tcccctgctc ctgctctcct cggtgatggt cgcggccggg
      121 atcggcctca ccgtccgtcg acctccccct ctcccccctg gggagagggc agggtgaggg
      181 cgagagggca ggatgagggg tgtcggctcc accttgaccg gtccccaccc ccgcggtagc
      241 gtcggctcat gcccagccag ccctcgaaga ccctcgaggt cgtccccaac ccgcatcccg
      301 accgcgacta cgaggtgagc ctcgagatcc cggagttcac ctgcatctgc ccgcgcaccg
      361 gccagcccga cttcgcgacc atccgggtgc gctacgtgcc ggaccagcgg ctgctcgagc
      421 tgaaggcgat caagctctac atctggtcct atcgcaacga gggcgccttc cacgaggacg
      481 tgaccaaccg gatcctgaac gacttcgtgg ccgccgcggc gccgcggtgg atcgaggtgg
      541 tgggcgactt cacggtgcgc ggcggcatca agaccgtggt gcgggccacc cacggcaagc
      601 ggccggagct ctagccggcc aggctcagca gcaccacccc ggccagcatc accagcgcgc
      661 cgggcgcgat gtcccgcacc ttctgcttct cgtggaacat gatcgtcccg atcaccatcg
      721 cgaacaggat ctccacctgc ttgaccgcct ccacgtagga ggacagcgtc atcaggtacg
      781 cggtcccctg gctcacggtg gtgagggacg cgaagagacc gaagctcacg aaggccttcc
      841 agtagcgcgg gatctccacg aagtgccgcc gcgaggtcac gagcacgatc ggggtgacga
      901 gcaggctcga ggccaggtag ccgccgaggg tgcccatgta ctggtcggag cactgcatcg
      961 cccacttgat cgtcaccacc gacggggcga agaagagggc ggcgagcagg gtgtagcgca
     1021 gcccgcggtc ggtgaacagc accgccagcg gggcccacca cgcgatgtgg gcccggttga
     1081 cgttgagcag gtacaccccg ctcatgctga tcaggatgcc caggatgccg agcagggtcg
     1141 gccgctcctt cagggtgaag tagccgagca cgaccagcac cagcaggctc accttccaga
     1201 gcgcggtgac catcgagatc tcggagagct tcagggcctt cgagagcgac atggtggaga
     1261 tcgtctggca gaccgcgaag cacacgcagg cgaggtagaa gccgggcttg atgaccggca
     1321 cgcccttcca gagcacgaag gcgcccgcga acggcagcag gaaggtgaag cggccccaga
     1381 cgttgatgta ctcgtcgagc gagtgcccga ggcgcttcat caccgtgttg cgcagcacct
     1441 ggcagagggc cgccaccagc gcgaggagga gccacatggc ggagcgctca gcgcgtccgc
     1501 gccagcagcg agaggagcgc ctccaccacc gcgccgggct gctcgcgcgg caggaagtgg
     1561 cccgcgtccg gcaccacgcg gcgctcggtg ccggccggga agaggtccgg gtgccgctcc
     1621 gacttctggg ggcggtcgac gccgtcgtcc ccgccgtgca ggatcacggc cggcacctcg
     1681 atgcgcggcc gctccgccag ccggcgctcg accgcgtcga agcgcgggtc gcccggcgcg
     1741 ttgcggtgcc gatgccggta ggagtggatc accaccggga cgaagtcggg gttgtcgaag
     1801 gccaccgcgg tgcgctcgaa cgtggcgtcg tcgaagcgcc agcccggcga ccagtcccgc
     1861 cacaggagac ggcagatctc gcgccggttc tgctcgagcc ccaggcgccc gcgctcggtg
     1921 ttgaagtacc actggtacca gtagccccgc tcgtcctccg cggaagccgg gcgcggcggg
     1981 gccagcgtgt tctgcacgtt gtagccgccg atggtgacga gcgcccgcac ccgcgccggg
     2041 gccaggatcg ccgcgaggca ggccgcgcgc ccgccccagt cgtagccggc gaggctcacg
     2101 cgctcgagac cgagcgcgtc catgaagtcg agcaggtcct ggccgatcgc ggcctgctga
     2161 gccatgcgcg gctcgccggg gtcgaggaag cgcgtcggtc cgtagccgcg caggtagggc
     2221 acgagcacgc ggcagccctg ggcggccagc gggccggtcg ccccgtccca ggcgcggacg
     2281 tcgtcgggaa agccgtgcag caggacaatc gggggaccgg tcgcggggcc gtgctcctcg
     2341 tagccgatct cgagggtcgg cgtgcggacg gtcgccacga gcggccggga gccgggctag
     2401 aagccgctga cgtacggcag gcgcttcgcg gtggcctcga tggcgtcgcc cgcctcgagc
     2461 aggacgccgg tgggattcca cgcgcccgac acgagctggt tgcgcgcccc gtccgggatc
     2521 acgcccttga tcacccgctc gcccacgcgc acctcctgct tctccacgtc caccgtcacc
     2581 ggctgcttgg ggtcgcgccc gaccgccttc tgcagccaga gcacgtcctc gttgtcgatc
     2641 cggacacacg ggatcccgag catggtgcag ttgccgaaga agatctcgcc gaaggaggcg
     2701 cccacgatgg ccttgatgcc ccactgcttg agcgattccg gcgcgtgctc gcgcgaggac
     2761 ccgcagccga agttgatccc ggccaccagc accgcggcgc cctcgtaagc cttctggttg
     2821 aagggatgct cgggatcgtg cttgcgcgcg tcctcgaagg cgtgcagccc gagcccctcg
     2881 ttcgtcaccg tcttgaggaa ccgcgccggg atgatccggt cggtgtcgat gtcgttgccg
     2941 ggcaccggaa tcgcccgccc cgcgacctgc cgccgcgcgc tctcgctcat cagagcatct
     3001 cccgcacgtc ggtgaccgcg ccgtggagcg cggccgccac ggccatggcc gggctcatca
     3061 gcagggtccg cccggtcggg ctcccctgcc gcccgatgaa gttgcggttg ctggtggagg
     3121 cgctcatctc gcggccccgc agcttgtcgt cgttcatgcc gaggcacatc gagcagcccg
     3181 cgttgcgcca ctcgaagccc gcctcgcgga agacctcgtg cagcccttcc gcctccgcga
     3241 ccttggccac ctgctgcgag cccggcacca cgatggcccg cacgcccttg gccacccggt
     3301 tgcccttggc caccgcggcg gccacccgca gatcggagat ccgcgagttg gtgcacgagc
     3361 cgatgaagac cacgtcgatc ttggcccccg cgatgggctg cccggcctcg aagcccatgt
     3421 gggccagtcc ctcgcggtag gtcgcccgct cctgctcggg cgccgtctcc ggattcggga
     3481 ggcgctcgcc cacgcccacc gactgccccg ggttgatgcc ccacgtcacc atcggcggga
     3541 tcgcggagcc gtcgagccgc ttgacgtcgt cgtatcgcgc gtccgggtcg gaggccatgc
     3601 cctgccacca gctgaccgcg cgatcgaacg cggcgccctg cggcgcgtac tggcggccgc
     3661 gcaagtagtc gtaggtggtc tggtccgggt tgacgtagcc gaagcgtgcc ccgccctcga
     3721 tcgacatgtt gcagagagtg agccgctcct ccatggtcat gcggtcgacg accggtcccg
     3781 cgtactcgta ggcgtagccc accccgccgt tcaccccgag ggtgcggatg atcgcgagga
     3841 tcacgtcctt ggcatagacg cccttggaga gcgcgccgtc cacccgcacc tgacgcagct
     3901 tcggcttggc catggccagg cactgggtgg ccagcacgtc gcgcacctgg ctggtgccga
     3961 tcccgaaggc gatggccccg accgcgccgt gcgtgctggt gtggctgtcg ccgcacgcga
     4021 tggtcatgcc gggctgggtc aggccgagct cggggccgat cacgtgcacg atcccctgct
     4081 tgccgctgtc caggccgaag aacggcaccc cgctctgctg ggcgttgcgc gtgaggtgga
     4141 ccgtcaactc ctcggccacc tcgtccgcga acggccgctg ctgggcggtg gtggggatga
     4201 tgtggtcgag ggtcgcgaag gtccgcgccg gcatccgcac cggcaccttc agctcgcgca
     4261 gggcctggaa cgcctgcggc gaggtgacct cgtggatcag gtgaagcccg atcaggagct
     4321 gcgtcgatcc cgagggcagc gtccggaccg tgtgggcgtc ccacaccttg tcgagcagtg
     4381 atgacggcat tggcatcctc cgtggccgcc gagtgtgccg cgacgtgacc gttaagtata
     4441 gtgaaatctt cttcccggtc acgtaaggtg gactacatga tcgacgcggc cctcctcccg
     4501 gccctgctga gcctgctggc cgtcgcccgc agcgggtcgg tgggagcggc ggcgcgcctg
     4561 cagcaccgca cctcgtccgc gatcagccag cagatccgca agctcgagcg ccatctggac
     4621 gtcaagctgc tcgagcgcgc gggccggggc gtgcggctca ccgcggccgg ggaggccgcg
     4681 ctccccggca tcctccagct cggcaccgag accgaggcgc tcttcgcgca gctcgccgag
     4741 ctgtccggcc gcccgcccgc gatcctccgg gtggcggtca gcgactacct gggccgcgac
     4801 ctgctggtgc cggtgctgca cgagctgctc gaggagcagc cgcgcctgcg cttcgagatc
     4861 gcgaccaccc actcgcgcga gagcgtgcgg ctggtgggcc tgggcgaggt ggactgcgcg
     4921 gtcgtcaccg gccaggaggc gccgcgcggc atcgagacgg cgcggctctt cgaccaggcg
     4981 ttcgtctggg tggggccgcg gcgcgcgcgc cgccgcgctc cgctggccga gcggctcgcc
     5041 cgcgagccgc tgctgcgcct cggggcgggc agtcagggcc ggcgcctgct cgaggactac
     5101 ctggcccgcg agcacatcca gccggtctcg acgatcgacg tgccgagcgt ctcgctgctc
     5161 ctgacctacg tctccggcgg cctcggggtg gggctcgccc ccgcgctggc cctggccgag
     5221 ggaggatccg ggcggcgggc ctacgactgg gagcgtgccg cggtgcccgc gctcaccgtc
     5281 acgctggtgc accggccggg cttccggcgg agctccgccg cggagcgctt cgcaacgcgg
     5341 ctcgcggccg agggccggcg cgcgggcgcg cgcctgcgcc gcatgggcct cggctaggcg
     5401 agcaggccgg gcagtgctga ggcctggaag ccggggaaaa ggggcccggc gggcgcgccg
     5461 aggtggcgca ccgcgacctc ggtgaagagc gcgcgaaagt cggtggtgac ggccaggtcg
     5521 cggccctcga agagctgctc gcgacggagc ccgggccacc gcccgtacac gcggccgccc
     5581 ctcaccccgc ccccgagggc cagcatcgcg gtggcgtgcc cgtggtcggt cccgcgattg
     5641 ccgttctcgc gcacggtgcg gccgaactcc gacatcgtga gcaccaccac gtcgctcatc
     5701 cgggcgccca ggtcacggtc cagcgcggcg agcccctcgc cgaactcgcg caggcgcgcc
     5761 gcgagctgac cgcgctcgtt gccctgcccc gcgtgggtgt cccagccgcc cacgtcggcg
     5821 aaggccacct cgagacccac gtcggcgcgg atcagctggg cgatctgccg gaggctgtcg
     5881 ccgaaccggc cccgcggata gaccgccccg ttggccggcc cgaggcgctg gggcgcggcc
     5941 gccttcagca tcttcaccgc ctcgaacgtc tcgcggccgg ttccatacag gagatcgcgc
     6001 acgcctttct cgtacatcga ctcgaagccc tgccgcgcgc cgggccgacc ggccatggcc
     6061 gcgccgccct ccttcacgtc gaaatcgctc acgctgctca tcgcgaccgc gcccacgtcg
     6121 ccgcggagga tgcgcgggag cgcggccccg agcgacaccg cgcggaacgg cgaggccggc
     6181 tcgcgggccg gcatcccggc gaggccgcgg gccagccagc cgtcgggcgt gctcttcacg
     6241 ccgggcgttc ccgactccat gtagtcctgc gcgtcgaagt gcgagcgggt ggtgtcggtc
     6301 gagccgcacg cgtggaccac cgcgaggcgc cggtcgtccc acagcggctt gaggggagcg
     6361 agcgacgggt gcaggccgaa gaaggcgtcg aggtccacgg tggcctcggc gacgcccgga
     6421 gccggccgcg cgatcgcgat ggagccgcgg accccgtagt agtcgggatc accgtgcggc
     6481 accaccatgc tgaggccgtc gaccgcgccg cgctggaaga t
//
LOCUS       DATMCV010000716         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_305288, whole genome shotgun sequence.
ACCESSION   DATMCV010000716 DATMCV010000000
VERSION     DATMCV010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305288"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VNU03_16430"
     CDS             <1..369
                     /locus_tag="VNU03_16430"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP76111.1"
                     /translation="VVRSLFLLPEVKKGMETALELDPRFPSAYVLAGTVYCEVPGLFG
                     GDLEKSEAIFRRGLEVDPRFTALRVGLARTLIKRRRIPEARRELQAALDEKSPGNLAD
                     WTLKDAPEARALLASLAGRS"
     gene            372..1013
                     /gene="nth"
                     /locus_tag="VNU03_16435"
     CDS             372..1013
                     /gene="nth"
                     /locus_tag="VNU03_16435"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWP76112.1"
                     /translation="MGHVIRRLRREAPTWRATALALVADQWHDPFRVLIACLLSLRTK
                     DETTGPAAARLFALADTPERLRRLSPRTIERAIYPVGFYRTKARVVRDVSRDLIERFG
                     GAVPDEIDALLTLKGVGRKTANLVVTQGFNKPGICVDVHVHRISNRWGYVTTRTPEET
                     ETALRRRLPRRYWIGYNDLLVSFGQNVCLPVSPHCSRCPIRAGCPRKGVRRSR"
     gene            complement(1010..1858)
                     /locus_tag="VNU03_16440"
     CDS             complement(1010..1858)
                     /locus_tag="VNU03_16440"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HWP76113.1"
                     /translation="MSIAGGLPLALTRGLEVGCSVVQIFLKNQRQWTARAYSDSEVRE
                     FRAAWKASGIRVVFAHANYLINLATPVPAEWHRAVDVFHDELERAEALGLPFVVIHPG
                     SHRATGLAEGVLRVARAIDVLHERTRGQRVRIVLENTAGGGASVGRSFEELAAVIGAV
                     REPERVGICLDTCHLFAAGYDIRSRAGYRATMTECARRLGLERVLAFHLNDAKQPLGS
                     GLDRHEKIGRGHLGVEAFRLLMTDPRFARVPMALETPKDPEPRADRDALALLRGLRRR
                     ARRARS"
     gene            complement(1875..>2231)
                     /locus_tag="VNU03_16445"
     CDS             complement(1875..>2231)
                     /locus_tag="VNU03_16445"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76114.1"
                     /translation="TVFFGEEANLPARFARSLAAQKDRYDKGVERIVAEGIRAGVFRD
                     VPPRLVVFAILGMVNWVHKWYNPRGRWGAEEISAAFLSLIEGGLLRQKPRGRALSGRL
                     RRLERELHEVARQLDA"
BASE COUNT          295 a          853 c          781 g          302 t
ORIGIN      
        1 gtggtgcgat ctctcttcct gttgcccgag gtgaagaagg gaatggagac cgcgctcgag
       61 ctggacccgc gcttcccgtc cgcctacgtg ctcgccggta ccgtctactg cgaggtgccg
      121 gggctcttcg gcggcgacct cgagaagtcg gaggcgatct tccggcgggg gctcgaggtc
      181 gacccgcgct tcaccgctct gcgcgtcggt ctcgcccgca ccctcatcaa gcgccggcgg
      241 atcccggagg cgcggcgcga gctgcaggcg gcgctcgacg agaagagccc cggcaacctg
      301 gccgactgga cgctcaagga cgcgcccgag gcgcgcgcgc tgctcgcgtc gctcgcgggt
      361 cgctcgtgag catcggccac gtcatccggc gcctccgccg ggaggcgccg acctggcggg
      421 cgaccgcgct ggccctggtg gccgaccagt ggcacgatcc gttccgcgtc ctgatcgcct
      481 gcctgctctc gctccgcacc aaggacgaga ccaccgggcc ggcggcggcc cggctcttcg
      541 cgctggccga cacccccgag cgcctgcgtc gcctgagccc ccgcaccatc gagcgggcga
      601 tctacccggt gggcttctac cggaccaagg cgcgggtggt gcgcgacgtg agccgcgacc
      661 tgatcgagcg cttcggcggc gcggtgccgg acgagatcga cgcgctcctc accctcaagg
      721 gcgtcggccg caagaccgcc aacctggtgg tgacccaggg cttcaacaag ccgggcatct
      781 gcgtggacgt gcacgtgcac cggatctcga accgctgggg ctacgtgacc acgcgcactc
      841 cggaggagac cgagacggcg ctccgccgcc ggctccctcg ccgttactgg atcggctaca
      901 acgatctgct ggtctccttc gggcagaacg tgtgcctgcc ggtgtcgccc cactgctcgc
      961 gctgcccgat ccgcgcgggc tgcccgcgca agggcgtgcg ccgctcccgc tagctccgcg
     1021 cgcggcgcgc gcgccgcctc agcccgcgga gcagggcgag cgcgtcccgg tccgcccgcg
     1081 gctcgggatc cttcggcgtc tcgagcgcca tcggcacccg cgcgaagcgt gggtcggtca
     1141 tcagcagccg gaacgcctcc accccgaggt gaccccgccc gatcttctcg tgccggtcga
     1201 ggccggagcc gagcggctgc ttggcgtcgt tgaggtggaa ggcgagcacc cgctcgagcc
     1261 cgaggcgccg cgcgcactcc gtcatcgtcg cgcggtagcc ggcgcgcgag cggatgtcgt
     1321 agccggccgc gaacaggtgg caggtgtcga ggcagatccc cacccgctcc ggctcccgca
     1381 ccgccccgat caccgcggcc agctcctcga agctgcgccc cacgctggcc ccgccgcccg
     1441 cggtgttctc gaggacgatc cgcacccgct gcccgcgcgt gcgctcgtgg agcacgtcga
     1501 tggcgcgcgc cacccggagc acgccctccg cgagcccggt cgcccggtgc gagccggggt
     1561 ggatcaccac gaagggcagc ccgagcgcct cggcgcgctc cagctcgtcg tggaagacgt
     1621 ccaccgcgcg atgccactcc gcgggtaccg gggtcgccag gttgatcagg tagttcgcgt
     1681 gggcgaagac gacccggatg ccgctcgcct tccaggccgc gcgaaactcc cgcacctcgc
     1741 tgtcgctgta ggcccgggcg gtccactgcc gctggttctt gaggaagatc tggacgaccg
     1801 agcagcccac ctcgagcccc cgcgtgagcg ccaggggcag gcctcccgcg atggacatgt
     1861 gggcgcccag ccagtcacgc gtcgagctgc cgggcgacct catgcagctc gcgctcgagc
     1921 cgacgcagac ggccgctgag ggcccgcccg cggggcttct gccgcagcag gccgccctcg
     1981 atcagggaca ggaaggcggc cgagatctcc tcggcgcccc atcggccgcg cgggttgtac
     2041 cacttgtgca cccagttgac catgccgagg atcgcgaaga ccaccaggcg cggcggcacg
     2101 tcgcggaaca ccccggcccg gatcccctcc gccacgatgc gctccactcc cttgtcgtag
     2161 cggtccttct gggccgcgag ggagcgggcg aagcgggcgg gcaggttggc ctcctcgccg
     2221 aagaacacgg t
//
LOCUS       DATMCV010000717         2837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_172860, whole genome shotgun sequence.
ACCESSION   DATMCV010000717 DATMCV010000000
VERSION     DATMCV010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172860"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNU03_16450"
     CDS             complement(<1..469)
                     /locus_tag="VNU03_16450"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP76115.1"
                     /translation="MPTPTPQPTPRLHVADARPNGAVPKLSGDIRHLIPKLGLRNYWY
                     PAVEARHVGRRKPVKVALLGEELCLFRGAAGEVVAIQDVCPHRGARLSEGDCHYQGTV
                     ACPYHGWVYDESGKNVMVLSEGPNSGVCGKAGTEAKAYPTRTVKGLVFVWIGEE"
     gene            complement(481..1536)
                     /locus_tag="VNU03_16455"
     CDS             complement(481..1536)
                     /locus_tag="VNU03_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HWP76116.1"
                     /translation="MGEILGLGMTHYPPLTGLDQNMASILRRVLQDPGLPERYRDPAS
                     WPAPMRAEYGSDGGTESAAGHRDALLHHFRKARKILDDFRPDVVVIWGDDQHENFTAD
                     VIPPFCVLAYDKVEARPWQRAGVGPNVWGEPSETSFQIMGHREAGKHLASGLLEQGVD
                     MAYAYKPLHYPGLAHAFLNTIMFLDYDRVGFPYPVIAFQVNCYGRRVIAQRGGRASLA
                     NPLGEADLDPPSPSPKRCMEVGAATARVMRDSPWRTALIASSSWSHAFLTDKNHQLYP
                     DIESDRRLYEALRVGDYETWRKTPLHAIESSGQQEMLNWYMLAGAMEALGRKPDECYF
                     LETWSFNSNKCFATFMP"
     gene            complement(1655..2419)
                     /locus_tag="VNU03_16460"
     CDS             complement(1655..2419)
                     /locus_tag="VNU03_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76117.1"
                     /translation="MRFEGKVALISAAGAGIGKATAMIIAREGGIVVGVDVEQAALDR
                     LAADVTSAGGRALGLRADALDAAAVEGVVKRAVDAHGRIDILVNAVGGSTVVSRPAAP
                     LDELTLEEWQKLLHFNLTGTFLFTHAVVPVMKRQGAGKIVNLASIAGRGVSQSSSSAY
                     ATAKGGIIALTRKLAFELGPHGVTVNAIAPSLTLSERIRPHWDRRSAEGQAAEIRRTP
                     LGRVATPEDQARVICFLASSDADFVTGVTIDVTGGQ"
     gene            2448..2627
                     /locus_tag="VNU03_16465"
     CDS             2448..2627
                     /locus_tag="VNU03_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018396462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HWP76118.1"
                     /translation="MTYDCVFCGEENDVLVDPSGGRHQTFTEDCTVCCRPNLITLTLD
                     DDGDVTIEVVQEYEA"
     gene            complement(2632..>2837)
                     /locus_tag="VNU03_16470"
     CDS             complement(2632..>2837)
                     /locus_tag="VNU03_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP76119.1"
                     /translation="RPSLGIPGFADTTTAMIPMRRLGRPEEIASVVMFLASDLASYVT
                     GAALVADGGLTAQTGMPSRIPLP"
BASE COUNT          405 a          971 c         1032 g          429 t
ORIGIN      
        1 gctcctcgcc gatccagacg aacaccaagc ccttgacggt gcgggtcggg tacgccttgg
       61 cctcggtgcc ggccttgccg cacacgccgg aattcggacc ctccgagagc accatcacgt
      121 tcttgcccga ctcgtcgtag acccagccgt ggtacgggca cgccaccgtc ccctggtagt
      181 ggcagtcgcc ctcgctgagg cgcgccccgc gatgcgggca gacgtcctgg atggccacca
      241 cctcgcccgc cgcgccacgg aacaggcaca gctcctcgcc gaggagcgcg accttgaccg
      301 gcttccgccg cccgacgtgg cgggcctcga ccgcggggta ccagtagttg cgcaggccga
      361 gcttcgggat caggtgccgg atgtcgccgc tcagcttggg aacggcgccg ttcgggcgcg
      421 cgtcggcgac gtgcaggcgc ggggtcggct ggggagtagg tgtcggcacg ccgatctcct
      481 tcagggcatg aaagtggcga agcacttgtt cgagttgaac gaccacgtct cgaggaaata
      541 acactcgtcg ggcttgcgtc caagggcctc catggcgccg gccagcatgt accagttcag
      601 catctcctgc tggcccgacg actcgatggc gtgcagcggc gtcttgcgcc acgtctcgta
      661 gtcgcccacg cggagggcct cgtagaggcg gcgatccgac tcgatgtcgg ggtagagctg
      721 gtggttcttg tcggtgagga aggcgtgcga ccagctcgac gacgcgatca gggcggtgcg
      781 ccaggggctg tcgcgcatca cccgcgcggt cgccgcgccc acctccatgc agcgcttcgg
      841 cgagggcgac gggggatcga ggtcggcctc cccgaggggg ttggccaggc tcgcgcgccc
      901 gccccgctgg gcgatcaccc ggcgcccgta gcagttcacc tggaacgcga tgaccgggta
      961 cgggaagccg acccggtcgt agtcgaggaa catgatcgtg ttgaggaagg cgtgcgccag
     1021 ccccggatag tggagcggct tgtaggcgta ggccatgtcg acgccctgct cgaggagacc
     1081 cgacgcgagg tgcttgccgg cctcccggtg gcccatgatc tggaacgacg tctcggacgg
     1141 ctcgccccag acgttgggcc cgacgcccgc acgctgccac ggccgcgctt ccaccttgtc
     1201 gtaggccagc acgcagaacg ggggaatgac gtccgcggtg aagttctcgt gctggtcgtc
     1261 gccccagatg accaccacgt cgggccggaa gtcgtcgagg atcttgcgcg ccttgcggaa
     1321 gtggtggagc agcgcgtcgc ggtgcccggc cgcgctctcg gtgccgccgt cggacccgta
     1381 ctcggcgcgc atcggggccg gccagctcgc gggatcccgg tagcgctcgg gcaggcccgg
     1441 atcctggagg acccggcgga ggatgctggc catgttctgg tccagaccgg tgagtggcgg
     1501 gtagtgcgtc atgcccagcc cgagaatctc gcccatggag gtggctcctc gccgtccggg
     1561 taaagcgctt gcgccaccct agtcccccgg tcccggcctt gtcaacttgg cccgcccggg
     1621 gggtgggcgg cctgcccggg gcggtgccgg tcggctactg gccgccggtc acgtcgatgg
     1681 tgacgccggt cacgaaatcg gcgtcggagg aggccaggaa gcagatgacc cgggcctggt
     1741 cctcgggggt ggccacccgc cccagggggg tgcgccggat ctcggcggcc tggccctcgg
     1801 ccgaccggcg gtcccagtgg ggtcggatgc gctcggagag ggtcaggctg ggggcgatgg
     1861 cgttgacggt gacgccgtgg ggccccagct cgaaggcgag cttgcgggtg agtgcgatga
     1921 tgccgccctt ggcggtcgcg taggcgctcg agctcgactg gctgaccccg cgcccggcga
     1981 tcgacgcgag gttgacgatc ttgccggcgc cctgccgctt catcaccggt accaccgcgt
     2041 gagtgaagag gaacgtcccg gtcaggttga agtggagtag cttctgccac tcctccaggg
     2101 tcagctcgtc gagcggcgcg gcggggcggc tcaccacggt gctgccgccc accgcgttga
     2161 ccaggatgtc gatgcgcccg tgggcgtcga cggcccgctt caccacgccc tccaccgcgg
     2221 ccgcgtcgag cgcgtcggcc cgcaggccga gcgcgcggcc ccccgcggag gtgacgtcgg
     2281 cggcgaggcg atcgagggcg gcctgctcca cgtcgacgcc caccacgatc ccgccctcgc
     2341 gggcgatgat catcgcggtc gccttcccga tgcccgcgcc cgccgccgag atcagcgcga
     2401 ccttaccctc gaatctcatg agctcttctc cgagtacact ggggatcgtg acctacgact
     2461 gtgtcttctg cggcgaggag aacgacgtgc tggtcgaccc gagcggcggc cgccaccaga
     2521 cgttcaccga ggactgcacg gtgtgctgcc ggcccaacct gatcacgctg acgttagacg
     2581 acgacggcga cgtgacgatc gaggtggtgc aggaatacga agcctgaccc atcagggcag
     2641 ggggatccgc gagggcatcc cggtctgcgc ggtcaggccg ccgtcggcca ccagcgccgc
     2701 cccggtgacg taggacgcga gatcggaggc gaggaacatc accacgctcg cgatctcctc
     2761 cggccgtccg aggcgccgca tcgggatcat cgcggtggtg gtgtccgcga agccggggat
     2821 gccgagcgac gggcgca
//
LOCUS       DATMCV010000718         3411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_637886, whole genome shotgun sequence.
ACCESSION   DATMCV010000718 DATMCV010000000
VERSION     DATMCV010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637886"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VNU03_16475"
     CDS             complement(<1..229)
                     /locus_tag="VNU03_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWP76120.1"
                     /translation="MIAPGRVYLVGAGPGDPGLMTVRGLELLRHAGVVVYDRLVNPAL
                     LDEAPPGTLRIFAGKRAGAHCLPQAQINALLI"
     gene            complement(226..861)
                     /locus_tag="VNU03_16480"
     CDS             complement(226..861)
                     /locus_tag="VNU03_16480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HWP76121.1"
                     /translation="MTASSYYPVFLDLADRPCLVVGGGPVAEGKVEGLLAAGARVTVV
                     SPTVTARLAGWAAEGRLTHLARAYRAEDLDGPQVAFAATDEAAVTRAVAADGRARSVW
                     VNAADEPARCDFILPSVMRRGRLVVAVSTGGASPAAARAIREELEGYLTEEHAALVEL
                     AAEARADLRRRGRAPGPAAWRGALDGRLRRLIADRRYRQARAHLARRLEPA"
     gene            complement(858..1517)
                     /locus_tag="VNU03_16485"
     CDS             complement(858..1517)
                     /locus_tag="VNU03_16485"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWP76122.1"
                     /translation="MTLRLGDIAPDFQAETTNGPIQFHEWLGDDWGVLFSHPRDFTPV
                     CTTELGYVARLRPEFERRGVRAIGLSIDPVASHKGWAADIQETQGYAVNFPIIADPDR
                     KVADLYGMIHPAHDEVYTVRTVFVIDPKKKVRLFITYPQTTGRNFDEILRVIDSLQLT
                     DRHRVATPVNWKPGEDVIIVPAVSDEEARAKFPAGWRALKPYLRLTPAPGREPAEPVK
                     R"
     gene            complement(1554..3251)
                     /locus_tag="VNU03_16490"
     CDS             complement(1554..3251)
                     /locus_tag="VNU03_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HWP76123.1"
                     /translation="MSEPVPETKAQRAERLKGALNPWAAYAEIERFARDGVGSIPPEW
                     LGTYFRWWGIYTQGDGVGAVGGKGGEGRAVPYFMLRIRIPNGFLTAAQLRTVADLAGR
                     YARGVADLTVRQNIQLHWVRVEDLPAIFSALGACSLSSLGTCGDVTRNITGCPLAGLD
                     GDELLDASPLVQAATAMVNGSPEFYNLPRKYKVSITGCRVWCSYPEINDVGLTAVRDL
                     YSGRVGFSLRVGGGLSTRPHLAPRLPAFVRPEQVLAVVKGVSEIFRDSDVLRQNREKA
                     RLKFLFLDHGWTPERFQSALEDRLGFRLDPAVPEVLPEEAYRDHVGVHPQKQEGLVYA
                     GLPILRGRLSPDEMRAIADLAERHGSGELRATTMQNLVVVNVPAARAAQLERDAAAAD
                     LRFEGSPFRRGTVACTGSEFCKLALTETKGFARWLVEDLEARLPGFQEHVRLHVTGCP
                     NSCGQHWIADIGIEGKKVKVDGRLVDAYYFCVGGAVGKHQAVARPVGYRVPAAHVPEA
                     VERLLRAYLAGRRDRQSFRAFCAAHTDDELRTFLAGTEIPAETRDAPAGPVPHGVDG"
BASE COUNT          466 a         1222 c         1250 g          473 t
ORIGIN      
        1 cgatcagcag cgcgttgatc tgggcctggg gcaggcagtg ggcccccgcg cgcttgcccg
       61 cgaagatccg gagcgtgccg ggcggcgcct cgtcgagcag cgcggggttg acgaggcgat
      121 cgtagacgac caccccggcg tgccgcagca gctcgagccc gcgcaccgtc atgagcccgg
      181 gatcgccggg ccctgcgccg accaggtaga cgcgcccggg cgcgatcatg ccggctcgag
      241 ccggcgcgcc aggtgcgcgc gggcctgacg gtagcgccgg tccgcgatga gccgtcgcag
      301 ccggccgtcg agcgcgccgc gccaggcggc cggacccggc gcccgcccgc gccggcgcag
      361 gtcggcccga gcctccgcgg ccagctccac cagcgccgcg tgctcctcgg tgagatagcc
      421 ctccagctcc tcgcggatgg cccgtgccgc cgccgggctc gcgccgcccg tggacaccgc
      481 gaccacgagc cggccgcgcc tcatcaccga gggcaggatg aagtcgcagc gcgccggctc
      541 gtcggccgcg ttgacccaga ccgagcgggc gcggccgtcg gcggccaccg cgcgggtgac
      601 cgccgcctcg tcggtggccg cgaacgcgac ctggggcccg tcgagatcct ccgcccggta
      661 cgcgcgcgcc agatgggtga ggcgtccctc ggcggcccag cctgcgagcc gcgcggtcac
      721 ggtggggctc accaccgtga cgcgcgcgcc cgcggccagc agcccctcca cctttccttc
      781 cgcgaccgga ccgccgccga cgaccaggca ggggcggtcg gccagatcga ggaagaccgg
      841 gtagtaggag ctcgccgtca tcgcttgacc ggctcggccg gctcccgccc gggcgccggc
      901 gtcagccgta ggtacggctt cagcgcgcgc cagccggccg ggaacttggc ccgcgcctcc
      961 tcgtcggaga ccgccggcac gatgatcacg tcctcgcccg gcttccagtt gaccggcgtg
     1021 gccacgcggt ggcgatcggt gagctggagc gagtcgatca cgcgcaggat ctcgtcgaag
     1081 ttccggccgg tggtctgggg ataggtgatg aagagccgca ccttcttctt gggatcgatc
     1141 acgaagacgg tgcggacggt gtagacctcg tcgtgggcgg gatggatcat gccgtagagg
     1201 tcggccacct tccggtccgg atccgcgatg atcgggaagt tcaccgcgta gccctgcgtc
     1261 tcctggatgt cggcggccca gcccttgtgc gaggccaccg gatcgatcga gagcccgatg
     1321 gcgcggacgc cgcggcgctc gaattcgggc cgcaggcgcg cgacgtagcc gagctcggtg
     1381 gtgcacaccg gcgtgaagtc gcgcgggtgg gagaagagca ccccccagtc gtcgcccagc
     1441 cactcgtgga actggatggg gccattggtg gtctcggcct ggaagtcggg agcgatgtcg
     1501 ccgagtcgaa gggtcatggt cgtctctcct tgtcggttcg gtcggtcggg gaatcagccg
     1561 tccacgccgt gcgggaccgg tcccgcgggg gcgtcgcggg tctccgcggg aatctcggtt
     1621 ccggccagga aggttcgcag ctcgtcgtcg gtgtgcgccg cgcagaacgc gcggaaggac
     1681 tgccggtcgc gccggcccgc gaggtaggcg cgcagcagcc gctccaccgc ctccggcacg
     1741 tgcgccgcgg gcacgcggta gccgaccggc cgcgccaccg cctgatgctt gcccaccgcg
     1801 ccgcccacgc agaagtagta ggcgtcgacc aggcggccgt ccaccttgac cttcttgccc
     1861 tcgatgccga tgtcggcgat ccagtgctgg ccgcagctgt tggggcagcc ggtgacatgg
     1921 aggcgcacgt gctcctggaa gccgggcagc cgcgcctcca gatcctccac gagccagcga
     1981 gcgaatccct tggtctcggt cagcgcgagc ttgcagaact cgctgccggt gcaggccacc
     2041 gtgccccgac ggaacggcga gccctcgaaa cgcaggtcgg ccgcggccgc gtcgcgctcg
     2101 agctgggccg cgcgcgcggc cggcacgttc accaccacga ggttctgcat ggtggtggcg
     2161 cgcagctcgc ccgagccgtg gcgctcggcc aggtccgcga tggcgcgcat ctcgtccggg
     2221 ctgagccggc cgcgcaggat cggcaggccc gcgtacacga gcccctcctg cttctgcgga
     2281 tgaacgccca cgtggtcgcg gtaggcctcc tcgggcagga cctcgggcac cgcggggtcg
     2341 aggcggaagc cgagccggtc ctcgagggcg gactggaagc gctccggggt ccatccgtga
     2401 tcgaggaaga ggaacttgag ccgggccttc tcgcggttct ggcggagcac gtcgctgtcg
     2461 cggaagatct ccgacactcc cttgaccacc gccagcactt gctccgggcg gacgaaggcg
     2521 gggaggcgcg gggccaggtg cggccgggtg gagaggcccc caccgacgcg cagcgagaag
     2581 ccgacgcggc ccgagtagag gtcgcgcacc gcggtgagac ccacgtcgtt gatctcgggg
     2641 tacgagcacc agacgcggca gccggtgatc gagaccttgt acttgcgggg caggttgtag
     2701 aactcggggc tgccgttgac catcgcggtc gcggcctgca ccagcggtga cgcgtcgagc
     2761 agctcgtcgc catcgaggcc ggcgagcggg cagccggtga tgttgcgggt cacgtcgccg
     2821 caggtgccga ggctcgacag cgagcaggcg ccgagagccg agaagatcgc gggcaggtcc
     2881 tcgacccgca cccagtggag ctggatgttc tggcgcaccg tgaggtcggc gacgccccgc
     2941 gcgtatcgcc cggccaggtc ggccaccgtg cggagctgcg ccgcggtgag gaagccgttg
     3001 gggatgcgga tgcgcagcat gaagtagggc accgcgcgcc cttccccgcc cttgccgccg
     3061 accgcgccca ccccatcgcc ctgggtgtag atgccccacc agcggaagta cgtcccgagc
     3121 cactcgggcg ggatcgagcc caccccgtcg cgcgcgaagc gctcgatctc cgcgtacgcg
     3181 gcccacggat tcagcgcccc cttgaggcgc tccgcccgtt gcgctttcgt ctccggtacc
     3241 ggctcgctca cgtgctcctc cttgcccgga aaaagcgaaa gccgaggagg gccgcgatct
     3301 ccgcggcccc cgctcggctt tctgtctcga tgctgcgact tgtgccttgt ctaggagtgc
     3361 ctcatccgat gtaccggagg ggtacgtcca cccatgcttt acagcgtgga c
//
LOCUS       DATMCV010000719         2478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_35634, whole genome shotgun sequence.
ACCESSION   DATMCV010000719 DATMCV010000000
VERSION     DATMCV010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35634"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VNU03_16495"
     CDS             <1..802
                     /locus_tag="VNU03_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HWP76124.1"
                     /translation="HERKLTRVLKAEGRPAHPEEVQKTLGVAPGSVGPLDVRGARIIA
                     DESLRDGRYVVGANREGFHVTGVKPGEDFTPEWADLQVALAGEGCPSCGKPLRIERVI
                     EVGNIFKLGTKYSVPLGATYLDERGQQQPIVMGSYGIGPARIAAAAVEQRHDADGIVW
                     PWAIAPFHVHLVPVSVKDAGQMAAAEELYRELRAAGFDVLMDDREERAGVKFKDADLL
                     GVPIRVTVGNALAKEGVVEVRQRATKADRRVPKAEVVGVIREMAQTIS"
     gene            943..1386
                     /gene="rimP"
                     /locus_tag="VNU03_16500"
     CDS             943..1386
                     /gene="rimP"
                     /locus_tag="VNU03_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWP76125.1"
                     /translation="MAPVLASRGLDLVDTEWHREGRRWVLRFFVDKPGGVGIQDCQSF
                     SREAGDVLDVSGLIEPSYDLEVSSPGLDRVLKKDRELRWAVGREVHCWVGEPVDGRTE
                     FSGRLVEVSPAALMLEEGVGTRREVPRRLVTKARLQLQAFGRTKG"
     gene            1386..>2478
                     /gene="nusA"
                     /locus_tag="VNU03_16505"
     CDS             1386..>2478
                     /gene="nusA"
                     /locus_tag="VNU03_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWP76126.1"
                     /translation="MNKDLIYVIEQIGREKGIGKEVLFEALESALLSASKKTMGAADN
                     VRMQIDRQTGSLRVFARKKVVGAVTDPKLEITLDEARRLNAAVEMEDEIEQELAPQEF
                     GRIAAQTAKQVILQRVRDAERDAVYAEFIDREGKIVRGAVHRIEKRNVVVELGKAEAV
                     ITEREQLPGERYNPGDRLRAYMLEVKKTAKGPQILLSRTHPGFLVRLFETEIPEIAEG
                     IVLVKAAAREPGERAKVAVSSTKRDVDPIGACVGLRGTRIQVISRELRGEKIDIVEWS
                     PDPAVFVARALSPAKVSSVTVEERPEGEEGDALRSVLVVVPDNQLSLAIGKKGQNARL
                     AAKLTGFRVDIKSEGEIEEERRRDEEERAE"
BASE COUNT          406 a          770 c          944 g          358 t
ORIGIN      
        1 ccacgagcgc aagctcaccc gggtgctcaa ggccgaggga cggccggccc atcccgagga
       61 ggtccagaag accctgggag tggccccggg ctcggtcggg ccgctcgacg tccggggcgc
      121 ccgcatcatc gccgacgagt cgctccgcga cggccgttac gtggtggggg ccaatcgcga
      181 gggcttccac gtgaccggcg tgaagccggg ggaggacttc actcccgagt gggcggatct
      241 ccaggtggct ctggccggcg agggttgccc gagctgcggc aagcccctcc gcatcgagcg
      301 ggtgatcgag gtcggcaaca tcttcaagct cgggaccaag tactcggtgc cgctgggagc
      361 cacctacctg gacgagcgcg gccagcagca gccgatcgtg atggggtcct acgggatcgg
      421 cccggcccgg atcgccgccg ccgcagtgga gcagcgccat gacgccgacg gcatcgtctg
      481 gccctgggcg atcgcgccct tccacgtgca cctggtgccg gtctcggtca aggatgccgg
      541 gcagatggcc gccgccgaag agctctaccg ggagctccgg gcggccgggt tcgacgtcct
      601 gatggacgac cgcgaggagc gggccggggt gaagttcaag gacgccgacc tgctgggggt
      661 gccgatccga gtgaccgtcg gcaatgccct ggcgaaggag ggcgtggtcg aggtgcgcca
      721 gcgcgccacg aaggcggatc gccgggtgcc caaggcggag gtcgtcggcg tgatccggga
      781 gatggcgcaa acgatttcat agaccatccg cgtctccgtc cccttgcagg ggtcggatcc
      841 ctgtgctaaa gtcctgaaaa aagtgggccg tcgcccactt ttttttctgg ggggcagggc
      901 atggtggatg atgcacagtg gctcgaccgg atcgaagcgg tcgtggcgcc ggtgctcgcc
      961 tcgcgcggcc tggacctcgt ggataccgag tggcaccgcg aggggcgtcg ctgggtgcta
     1021 cgcttcttcg tggacaagcc gggcggtgtc ggcatccagg attgccagtc cttcagccgg
     1081 gaggccggcg acgtgctgga cgtctccgga ctgatcgagc cctcctatga cctcgaagtg
     1141 tcctcgcccg ggctggatcg ggtgctgaag aaggatcgcg agctccgctg ggcggtcggc
     1201 cgtgaggtac actgctgggt cggagagccg gtggacgggc gaaccgagtt ctcggggcgg
     1261 ctggtggagg tgtctccggc cgcgttgatg ctggaggaag gtgtcggaac acggcgcgag
     1321 gtaccgcgcc ggctggtgac gaaagcgcgc ctgcagcttc aggcgtttgg gcggaccaag
     1381 ggatgatgaa caaagatctc atctacgtta tcgagcagat cggccgcgag aaggggatcg
     1441 ggaaagaggt cctcttcgag gcgctggagt ctgccttgct gtcggcgtcc aagaagacca
     1501 tgggcgcggc ggacaacgtc cggatgcaga tcgaccgcca gaccggaagc ctgcgcgtct
     1561 tcgcccgcaa gaaggtggtg ggcgcggtca ccgatcccaa gctcgagatc accctcgacg
     1621 aggcccgccg gctcaacgcc gcggtcgaga tggaggacga gatcgagcag gagctcgcgc
     1681 cccaggagtt cgggcgcatc gcggcccaga ccgccaagca ggtgatcctg cagcgggtcc
     1741 gcgacgccga gcgcgacgcg gtctacgccg agttcatcga ccgcgagggc aagatcgtcc
     1801 gcggcgcggt gcatcgcatc gagaagcgga acgtggtggt ggagctgggc aaggcggagg
     1861 cggtgatcac cgagcgcgag cagctgccgg gcgagcgcta caacccgggc gaccgtctgc
     1921 gggcctacat gctggaagtc aagaagacgg ccaagggccc ccagatcttg ttgtcgcgca
     1981 cgcatcccgg cttcctggtg cggctgttcg agaccgagat ccccgagatc gcggagggca
     2041 tcgtgctggt caaggccgcg gcccgcgagc ccggcgagcg cgccaaggtg gcggtgtcgt
     2101 cgaccaagcg cgacgtggat ccgatcgggg cgtgcgtcgg gctgcgcggc acgcgcatcc
     2161 aggtgatcag ccgcgagctg cgcggggaga agatcgacat cgtggagtgg tcgcccgacc
     2221 cggcggtctt cgtggcccgg gccctctcgc cggccaaggt ctcctcggtc acggtggagg
     2281 agcggccgga gggcgaggag ggcgacgccc tgcgctcggt gctggtggtc gtccccgaca
     2341 accagctctc gctcgccatc ggcaagaagg ggcagaacgc ccggctcgcc gcgaagctca
     2401 ccggcttccg cgtcgacatc aagagcgagg gcgagatcga ggaggagcgg cgccgcgacg
     2461 aggaagagcg cgccgagg
//
LOCUS       DATMCV010000720         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_572719, whole genome shotgun sequence.
ACCESSION   DATMCV010000720 DATMCV010000000
VERSION     DATMCV010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572719"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(526..972)
                     /locus_tag="VNU03_16510"
     CDS             complement(526..972)
                     /locus_tag="VNU03_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HWP76127.1"
                     /translation="MKIEGSADIPAARDKVWAAFLDPAILAQALPGCEKLEAIGPNEY
                     KATMKVGVAAIKGTFEGKVKLSDLEPPHRYRMAVEGSGGPGFVRGDAGMQLSDVDGGT
                     RVSYDADVQVGGLIASVGQRMLGGVTKMMLDQFFTRMTELLTAQKA"
     gene            complement(973..2166)
                     /locus_tag="VNU03_16515"
     CDS             complement(973..2166)
                     /locus_tag="VNU03_16515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWP76128.1"
                     /translation="MLADRMKTLAPSPTLAMQAKARAMRAQGIDVISFGAGEPDFDTP
                     ARIKAAAIRALETGQTKYTEVGGVPELRAAVCQKLKRDHGLEYAPDEVTVSCGAKHTL
                     YNIVMALVNPGDEVLIPSPYWVSYPEQVQLLGGVPVPVPTHESTGFDLDPAALRAAVT
                     ARTKIIVLDSPGNPTGAVFSPEALAQVARLAVERDLWVISDECYESLTYEGRHVSIAS
                     LSPEIKARTIVVNTCSKAYAMTGWRIGYAAGPKPIIRAMTDVQSQVTSNPSSVAQWAA
                     VEALGGPQDEVAKMAGEFDRRRRFIVPALNAIPGVTCVMPKGAFYAFPNVSGLFGRRG
                     RGGVLRGSADVSAFLLDEARIATVAGVDFGSDAHIRLSYATSLDKIQEGLRRMDTAVR
                     SLQGA"
     gene            complement(2178..>2541)
                     /gene="coaD"
                     /locus_tag="VNU03_16520"
     CDS             complement(2178..>2541)
                     /gene="coaD"
                     /locus_tag="VNU03_16520"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWP76129.1"
                     /translation="LFTAKERLELIDECTTGIGMSRMRITSFDGLLIDLVDREGADCV
                     VRGLRAVSDFEYEFQMALMNRKLRHTVETVFMMPHERYTYISSRLIKEVALLGAPITG
                     MVPPAVEERLAQKFASRR"
BASE COUNT          363 a          868 c          928 g          382 t
ORIGIN      
        1 ggtgagcgca gctcggagtg cgaggccggg ggcccagtcg cgcgcgatgc gggcgacctc
       61 gcgcgccgag tagatctggc gcaccgcgag cgcgcgcccg tccacctgcg ccatcagcag
      121 gcggtgcgcg tccaccgcga gcacgtcggg ctgtggcgac gagaggcggc gccgcttggc
      181 ctcgaagaag tacgagatgt cgccggtcgt gcctcccgcg aatcggatct gcgcgaaggt
      241 gggctcgtgc cagatgactt cggggcgcgg cagggtggcg acgccgggcg gcggctcgag
      301 ccagaccggc ttctcgggat cgccgtagta gtagccgacc cggcagtcac ggtcggtgcg
      361 gcaccgggag acgaggcccg tgctggggaa ggccgcgcgg tagatgcagt gcgagggctg
      421 gtcgggtgag cccgcggcgc agtctcccgc gaccggttgc ggggtggcgg cggccgcgat
      481 cgagagcagg cccgccagca ggagcgccga cgccgtggcg gccctttacg ccttctgcgc
      541 ggtgagcagc tcggtcatgc gggtgaagaa ctggtcgagc atcatcttgg tgacgccgcc
      601 cagcatgcgc tggccgacgc tcgcgatcag cccgccgacc tgcacgtccg cgtcatagct
      661 cacccgggtg ccgccgtcca cgtcggagag ctgcatgccc gcgtcgccgc ggacgaagcc
      721 cggccccccg ctcccctcca ccgccatgcg atagcggtgc ggcggctcga ggtcgctcag
      781 cttgaccttg ccctcgaacg tgcccttgat cgcggcgacg cccaccttca tggtggcctt
      841 gtactcgttc ggcccgatcg cttcgagctt ctcgcagccg ggcagcgcct gggccaggat
      901 ggcgggatcc aggaacgcgg cccagacctt gtcgcgcgcg gccgggatgt cggcggagcc
      961 ctcgatcttc atctaggcgc cctggagaga gcgaaccgcg gtgtccatcc gccgcaggcc
     1021 ctcctggatc ttgtcgagcg atgtcgcgta cgagaggcgg atgtgggcgt cggagccgaa
     1081 gtccaccccg gccaccgtgg cgatgcgggc ctcgtcgagc aggaacgcgg agacgtcggc
     1141 ggagccgcgg agcacaccgc ccctgccgcg cctgccgaag agcccggaca cgttcgggaa
     1201 ggcgtagaac gcccccttcg gcatcacgca ggtcaccccg gggatcgcgt tgagggcggg
     1261 cacgatgaag cggcggcggc ggtcgaactc ccccgccatc ttcgcgacct cgtcctgcgg
     1321 gccgcccagc gcctcgaccg cggcccactg ggccaccgag gaggggttgg acgtcacctg
     1381 gctctgcacg tcggtcatcg cgcggatgat cggcttgggg ccggccgcgt agccgatgcg
     1441 ccagccggtc atcgcgtacg ccttcgagca cgtgttgacc acgatggtgc gcgccttgat
     1501 ctcgggcgag agcgaggcga tcgagacgtg gcggccctcg taggtgagcg actcgtagca
     1561 ctcgtctgag atcacccaga ggtcacgctc gaccgcgagc cgggccacct gcgcgagcgc
     1621 ttccggggag aagaccgcgc cggtcgggtt cccggggctg tcgagcacga tgatcttcgt
     1681 gcgggccgtg accgccgccc gcagcgcggc gggatcgagg tcgaacccgg tcgactcgtg
     1741 cgtcggcacc ggcaccggca cgccgcccag gagctgcacc tgctccgggt acgagaccca
     1801 gtacgggctc gggatcagca cctcgtctcc cgggttcacg agcgccatca cgatgttgta
     1861 gagcgtgtgc ttcgccccgc aggagacggt cacctcgtcc ggcgcgtact cgaggccgtg
     1921 gtcgcgcttg agcttctggc agaccgcggc gcgcagctcg ggaacgcccc cgacctcggt
     1981 gtacttggtc tgccccgtct ccagcgcgcg gatcgcggcg gccttgatcc gcgcgggggt
     2041 gtcgaagtcg ggctcccccg cgccgaagga gatcacgtcg atgccctgcg cgcgcatggc
     2101 acgggccttg gcctgcatgg cgagggtggg cgaaggggca agggtcttca tccggtcggc
     2161 gagcatggtg tcctttctca gcggcggcta gcgaacttct gggcgaggcg ctcctccacc
     2221 gccggcggca ccatgccggt gatgggagct cccagcaacg cgacttcctt gatgaggcgc
     2281 gaggagatgt aggtgtagcg ctcgtgcggc atcatgaaca ccgtctccac ggtgtggcgg
     2341 agcttgcggt tcatgagcgc catctggaac tcgtactcga agtcggagac cgcccggagg
     2401 ccgcgcacca cgcagtcggc gccctcgcgg tccaccaggt cgatgaggag accgtcgaag
     2461 gaggtgatgc gcatccggct catcccgatg ccggtcgtgc actcgtcgat cagctcgaga
     2521 cgctccttgg cggtgaacag c
//
LOCUS       DATMCV010000721         3100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_35906, whole genome shotgun sequence.
ACCESSION   DATMCV010000721 DATMCV010000000
VERSION     DATMCV010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35906"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..553
                     /locus_tag="VNU03_16525"
     CDS             35..553
                     /locus_tag="VNU03_16525"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP76130.1"
                     /translation="MLTAADFIRANIKRLHDGLDKVMADLTPDQLHTVPAGHPKANTI
                     AWGVWHYTRTEDNIVRYVIQGRKPPIWVEGGYGERLGLHPAAQGTGMSTAEAQALRIK
                     DVPLFREYMGKVWAGTDELIAGAPPELLDKVITVKPLGDMSVVQCLGQVGLTHGMGHL
                     GEIELARTLIGA"
     gene            564..1622
                     /locus_tag="VNU03_16530"
     CDS             564..1622
                     /locus_tag="VNU03_16530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP76131.1"
                     /translation="MRFGWLTLSHSPSPDGDHAAIEEQLEQACAAETLGFDSVWLTEH
                     NFTGEAVYCDPIPFASALAARTSRVRIGFAVIQLALRHPIRLAVQLALLDNLSRGRLD
                     VGVGRGTLYNEYEFVGYGLRSDDSRERSAEALEVLTRAWTEEPFVHHGKFFQGSFPAL
                     RPRVYQRPHPPIWRSVVTPASFAECGRQGVPILTSRIPLGTIPERLRLYEEGLAAGGH
                     PARLREDVAVWRHVYVAPSRAQAEDELAETLAHTRRHMLEARAANNPQDYRVDPAVIN
                     PFNDPRVSEDEAVRFSLGTGALYGTPAEVAEQVAALREAGVHHVLAQMSFGYLGHGRI
                     MESMRRFGAEVMPAFRDR"
     gene            1718..2959
                     /locus_tag="VNU03_16535"
     CDS             1718..2959
                     /locus_tag="VNU03_16535"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWP76132.1"
                     /translation="MRLRAMGHGWRLAVVVAGIGLVAGGAAAVARAARNETYQALDVY
                     TEAMTIIHNQYVDELPWSKIVQDGIRGAVEGLDADSTVVEARKPAGAGDVGLLLTHRT
                     GALDVMATQDDSPARAAGLVPGDRIVLIDRVETRSLSVGDAAERLQGAPGSQVALSVF
                     RAGWAEPRDFRLTRAKPPATAVRSRALGAGVLYVRLPEITGRTPADLQRSIGAASGPV
                     TGLVLDLRNTVGGDVRAVPAVASLFLDPGCVVARVQSRVAGQSAELTTSAGPARFTQP
                     VTILVGQGTASAAEVLAAAMQDAKRAVIVGAPTFGDASTQSTTPLADGSRVSLTTSRY
                     ATPGGRPITGHGIAPDVVASEPAPDAAPAAGTTASIADPALQLALDVVKAAAILQPGA
                     SEPAAPTRIQTTAPRCSVSPA"
BASE COUNT          419 a         1147 c         1126 g          408 t
ORIGIN      
        1 cccatgatac aagggcagcc gaggagagac ccccatgctc accgcggcgg acttcattcg
       61 ggcgaacatc aagcggctcc acgacggcct cgacaaggtg atggccgacc ttaccccgga
      121 ccagctccac accgtcccgg ccgggcatcc gaaggccaac accatcgcgt ggggcgtctg
      181 gcactacacg cgcaccgagg acaacatcgt ccgctacgtg atccagggcc ggaagccgcc
      241 gatctgggtg gagggcggct acggcgagcg gctcggcctg cacccggcgg cccagggcac
      301 cggcatgtcc accgcggagg cgcaggccct ccggatcaag gacgtgccgc tcttccgcga
      361 gtacatgggg aaggtctggg ccggcaccga cgagctgatc gcgggcgcgc cgccggagct
      421 gctcgacaag gtcatcacgg tcaagccgct cggggacatg tcggtggtcc agtgcctggg
      481 gcaggtgggc ctcacccacg gcatgggcca cctgggcgag atcgagctgg cccgcacgct
      541 gatcggcgcc tgacccgccg cccatgcgct tcggctggct gaccctgtca cactcgccgt
      601 cgcccgacgg cgatcacgcg gcgatcgagg agcagctgga gcaggcctgc gcggcggaga
      661 ctctcggctt cgactcggtg tggctcaccg agcacaactt caccggcgag gcggtctact
      721 gcgacccgat cccgttcgcg agcgcgctgg ccgcccgcac ctcgcgcgtg cgcatcgggt
      781 tcgcggtgat ccagctcgcg ctgcgccacc cgatccgcct cgcggtgcag ctcgcgctcc
      841 tcgacaacct gagccggggg cggctcgacg tgggggtcgg ccggggcacg ctctacaacg
      901 agtacgagtt cgtgggctac gggctccgca gcgacgacag ccgcgagcgg agcgcggagg
      961 cgctcgaggt gctgacccgc gcctggaccg aggagccgtt cgtccaccac gggaagttct
     1021 tccaggggag cttccccgcg ctgcgcccgc gcgtctacca gcgcccgcat ccgccgatct
     1081 ggcgcagcgt ggtgaccccg gcgtccttcg cggagtgcgg gcggcagggc gtgccgatcc
     1141 tcacctcgcg catcccgctc ggcaccatcc ccgagcggct gcgcctctac gaggagggcc
     1201 tcgccgcggg cggccatccg gcgcgcctgc gcgaggacgt ggcggtgtgg cgccacgtct
     1261 acgtggcccc gagccgggct caggcggagg acgagctggc cgagacgctg gcccacaccc
     1321 gccgccacat gctggaggcc cgcgccgcca ataatccgca ggactaccgc gtggatcccg
     1381 cggtgatcaa tccgttcaac gacccgcggg tgtcggagga cgaggcggtg cgcttctcgc
     1441 tcggcaccgg cgccctctac ggtacaccgg ccgaggtggc cgagcaggtg gccgcgctgc
     1501 gggaggcggg ggtgcaccac gtgctggccc agatgagctt cggctatctc gggcacgggc
     1561 gcatcatgga gtcgatgcgc cgcttcggcg ccgaggtgat gcccgccttc cgggaccgct
     1621 agccgcgccc tggccgcggc gcggtccgac ccgcgccgcg accgtttctc atctgatcct
     1681 catcgatctc tcatctggca gtgctatccc ggatgccatg cgccttcgag cgatgggaca
     1741 cgggtggcgg ctggcagtgg tggtggcggg gatcgggctg gtggccggag gcgcggcggc
     1801 ggtcgcccgg gccgcccgga acgagaccta ccaagcgctc gacgtgtaca ccgaagcgat
     1861 gacgatcatc cacaaccagt acgtcgacga gctgccgtgg tcgaagatcg tgcaggacgg
     1921 aatccgcggc gcggtcgagg gcctcgacgc cgacagcacg gtggtggagg cgcgcaagcc
     1981 cgcgggcgcg ggcgacgtcg gtctgctgct cacccataga accggcgccc tcgacgtgat
     2041 ggccacccag gacgacagcc cggcgcgcgc ggccgggctc gttcccggcg accggatcgt
     2101 cttgatcgat cgcgtcgaga cgcgcagcct gagcgtcggc gacgcggccg agcggctgca
     2161 gggcgcgccc ggtagccagg tcgcgctcag cgtgttccgt gccgggtggg ccgagccgcg
     2221 ggacttcagg ctcacgcggg ccaagccgcc cgcgaccgcg gttcgctccc gggccctggg
     2281 cgcgggcgtg ctgtacgtgc gcctccccga gatcaccggc cgcacccccg cggatctcca
     2341 gcgctcgatc ggcgccgcct ccgggccggt caccgggctg gtgctcgatc tgcgcaacac
     2401 ggtgggcggc gacgtgcgcg cggtgccggc ggtggcctcc ctctttcttg atccgggctg
     2461 cgtggtggcc cgggtgcaga gccgcgtcgc cgggcagtcg gcggagctga ccacctccgc
     2521 cggcccggcg cgcttcaccc agccggtgac gatcctggtg ggccagggca ccgcgtcggc
     2581 cgccgaggtg ctcgcggccg cgatgcagga cgccaagcgg gcggtcatcg tgggcgcccc
     2641 cacgttcggc gacgcctcca cccagtcgac gacgccgctg gcggacggct cgcgcgtgtc
     2701 gctcaccacg tcgcgctacg ccactccggg cggccggccg atcaccgggc acggcatcgc
     2761 gccggacgtg gtcgcgagcg agcccgcgcc cgacgccgcg cccgcggcgg gcacgaccgc
     2821 ctccatcgcc gatcccgcgc tgcagctcgc gctcgacgtg gtgaaggccg cggcgatcct
     2881 ccagcccggc gcgtcggagc cggccgcgcc cacccgcatc cagacgaccg ctccgcgctg
     2941 ttccgtctcg cccgcctgac gtccgggctc ccggcggtag accgccggtg tagagtgtgg
     3001 ccgggaggag cccgcgcatg cgaatcgcca acgacgtcac ggagctggtc ggcaacaccc
     3061 cgctggtccg gatccgccgg ctgaccgagg gcgcgaaggg
//
LOCUS       DATMCV010000722         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_374452, whole genome shotgun sequence.
ACCESSION   DATMCV010000722 DATMCV010000000
VERSION     DATMCV010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374452"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VNU03_16540"
     CDS             <1..432
                     /locus_tag="VNU03_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWP76133.1"
                     /translation="RLDQSTLRVHAAKRLVGSDVTTEEYPGFATDMQAQYMALATQAE
                     GTSIVTENIFENRFMHAQELVRMGANIKIEGRRAIVRGRTPLSAAAVLASDLRASASL
                     VLAGLAARGNTEISRVYHLDRGYERLEVKLRSLGATIDRVK"
     gene            432..1097
                     /gene="hisG"
                     /locus_tag="VNU03_16545"
     CDS             432..1097
                     /gene="hisG"
                     /locus_tag="VNU03_16545"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWP76134.1"
                     /translation="MLTLALPKGRLLEPALTLLAGMGIRGFEPDSRRLLQADDAAGLR
                     FILLKPADVPTYVEHGAADLGIVGKDILLEQQPDVYEPVDLGFGFCRLVVAETRELWQ
                     RDDPAKWSWVRVATKYPRLTESYFSERGIQVEMVRLDGSIELAPLVGLAERIVDLVQS
                     GETLRANGLVEVAEILTSTARLIVNRASLKTAHAHVSQLIATMREVAGRAAAHGEPSK
                     YKK"
     gene            1094..>2025
                     /gene="hisD"
                     /locus_tag="VNU03_16550"
     CDS             1094..>2025
                     /gene="hisD"
                     /locus_tag="VNU03_16550"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWP76135.1"
                     /translation="MIRTLETSSSGIAGVIRALERPPGAVDPEIARRVAEIVAAVRST
                     GDAALLDCTRRFDRVALSAADLAVRPEEYAAAERAVGEVTLRSLRYAADRIERFHREC
                     APRSWRMRDDHGSRLGQEVRPLDRVAVYVPGGRAAYPSTVLMTVVPARVAGVREIVLA
                     SPPGADGSLNPAVLAAARIAGVTEAYRVGGAQAIAALAYGTATIRRVDKIVGPGNIYV
                     ALAKAQVFGDVGIDMVAGPSEVLVVADESADAEFVAADLLAQAEHDPMARAVLLTPSP
                     ALRDAVAAALGRQLAALPRREIAGAALAGHGALVL"
BASE COUNT          272 a          749 c          724 g          280 t
ORIGIN      
        1 cggctggatc agtcaacctt gcgcgtccac gcagccaagc ggctggtcgg ctccgatgtg
       61 accacagagg agtatcccgg cttcgccacc gacatgcagg cgcaatacat ggccctcgcc
      121 acccaagccg aaggcacaag catcgtcacc gaaaatattt ttgagaatcg cttcatgcac
      181 gcgcaggagt tggtccgcat gggcgctaac atcaagatcg aaggtcgccg cgccattgtt
      241 cgtggaagga cgccgttgag cgccgccgcc gtgctcgcct cagacttgcg cgcctccgcg
      301 tcgctggtgc tggccggcct cgcggcccgc ggcaataccg agatctcgcg cgtctaccac
      361 ctcgatcgcg gctacgagcg gctcgaggtc aagctgcgct cgctcggcgc caccatcgac
      421 cgcgtgaagt gatgctcacc ctggcgctgc ccaagggtcg gctgctggag cccgcgctga
      481 ccctgctcgc gggcatgggc atccgcggct tcgagcccga ctcgcggcgc ctcctccagg
      541 ccgacgacgc ggcggggctg cgcttcatcc tgctcaagcc ggccgacgtc ccgacctacg
      601 tcgagcacgg cgcggccgac ctcggcatcg tgggcaagga catcctgctc gagcagcagc
      661 ccgacgtcta cgagccggtc gacctgggct tcggcttctg ccgcctggtg gtcgcggaga
      721 cgcgcgagct gtggcagcgc gacgatccgg ccaagtggtc ctgggtgcgg gtggccacca
      781 agtacccgcg cctcaccgag tcgtacttct ccgagcgcgg aatccaggtc gagatggtcc
      841 ggctcgacgg ctccatcgag ctggccccgc tggtcgggct ggccgagcgg atcgtcgacc
      901 tcgtgcagtc cggcgagacg ctgcgggcga acgggctggt ggaggtggcc gagatcctca
      961 cctcgaccgc gcggctcatc gtcaaccgcg cctcgctcaa gaccgcccac gcccacgtga
     1021 gccagctcat cgccacgatg cgagaagtgg ccgggcgcgc ggccgcccac ggcgagccga
     1081 gcaagtacaa gaaatgatcc ggacgctcga gacgtcgagc tcggggatcg ccggggtgat
     1141 tcgcgccctc gagcggccgc ccggcgcggt ggacccggag atcgcccgcc gggtcgccga
     1201 gatcgtggcc gcggtccgct ccaccggcga cgcggccctg ctcgactgca cccgccgctt
     1261 cgaccgcgtc gccctctccg ccgccgacct cgcggtgcgg cccgaggagt acgcggcggc
     1321 cgagcgcgcg gtgggcgagg tgaccctccg ctcgctccgc tacgcggccg accgcatcga
     1381 gcgcttccat cgcgagtgcg cgccgcgctc ctggcgcatg cgcgacgacc acggctcccg
     1441 gctcggccag gaggtgcgcc cgctcgaccg ggtcgcggtc tacgtgccgg gcgggcgggc
     1501 cgcctatccg tccacggtgc tgatgacggt ggtgccggcg cgggtcgccg gggtgcggga
     1561 gatcgtgctg gcctcgccgc ccggcgccga cggctcgctg aacccggcgg tgctggccgc
     1621 cgcccgcatc gcgggcgtga ccgaggccta ccgggtcggc ggagcccagg cgatcgccgc
     1681 gctcgcctac gggaccgcga ccatccgccg ggtcgacaag atcgtgggcc ccggcaacat
     1741 ctacgtggcg ctcgccaagg cgcaggtgtt cggcgacgtg ggcatcgaca tggtggcggg
     1801 gccgagcgag gtcctggtgg tggccgacga gagcgcggac gcggagttcg tggccgccga
     1861 cctgctggcc caggccgagc acgacccgat ggcgcgggcg gtgctgctca cgccctcgcc
     1921 cgcgctacgc gacgcggtcg cggccgcgct cggccgccag ctcgccgcgc taccccgccg
     1981 cgagatcgcg ggcgcggcgc tcgcgggcca cggcgcgctg gtgct
//
LOCUS       DATMCV010000723         5069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_573165, whole genome shotgun sequence.
ACCESSION   DATMCV010000723 DATMCV010000000
VERSION     DATMCV010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573165"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..877
                     /locus_tag="VNU03_16555"
     CDS             212..877
                     /locus_tag="VNU03_16555"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP76136.1"
                     /translation="MANPVSPVGGTLTPDDPATLSAIRRYEERLARDPTSLAFAPLAD
                     AYRKTGRVRDAIRLCREGLERFPHYVTARLILSKALLDDGDPDGALSEVRAILEVSPH
                     EAQAHRLAGDLYRRSGALADAVSHLEHAVRLDPGDRESRALLDVLGAGGSVSDNSALK
                     QLLADDTFVTMSFGRVCLEQGLVDEAAQIFLRLLGRDLRHVGAREGLEEALRLKTQRR
                     KGS"
     gene            885..1451
                     /gene="efp"
                     /locus_tag="VNU03_16560"
     CDS             885..1451
                     /gene="efp"
                     /locus_tag="VNU03_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWP76137.1"
                     /translation="MVSTAEFKKGLKIRFDGEPYSIVDFQHVKPGKGGAFVRTKLKHM
                     RLGRVIDNTFRAGEKVELVDFEDKRMQYLYHDDRFHFMDLDTYDQISLSAEEVGEARD
                     FLKENIEVEILFINDSPITVELPNFIELQIAKTDPGIRGDTASGGSKPATLETGAVVQ
                     VPLFLNEGDVVKVDTRSGEYLGRVATAG"
     gene            1451..1927
                     /gene="accB"
                     /locus_tag="VNU03_16565"
     CDS             1451..1927
                     /gene="accB"
                     /locus_tag="VNU03_16565"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HWP76138.1"
                     /translation="MAARRPARGADGSGRVTLDQVVELAVRHNLAEIEVEAAGLRIRV
                     VREHAPAAGGSHGDVSSAAALPQPIPERAESTAHLVPVEAPMVGTFYRAPKPDAPPFV
                     AEGDVVKEGQVLCIVEAMKLMNEIEAKVSGRIVKVVVENGQPVEFGQSLFLMEPLR"
     gene            1959..3293
                     /gene="accC"
                     /locus_tag="VNU03_16570"
     CDS             1959..3293
                     /gene="accC"
                     /locus_tag="VNU03_16570"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWP76139.1"
                     /translation="MFHKILIANRGEIALRIIRACREMGIRTVIAHSTADAASLPVRL
                     ADESVCIGPPEARGSYLNIPSIISAAAITDSEAIHPGYGLLSENPAFAEICRACGITF
                     IGPSPEAIRLMGDKAQAREMAKQAGAPVVPGSEGPLGGVDEAQALADSMGYPVIVKAA
                     AGGGGRGMRIVREREGLARAYATCQAEAGAAFGSSELYLEKFVEEARHVEIQVLGDKN
                     GMRVHLGERDCTVQRRHQKLLEESPAPALAPETRAGLYKAALAVANAVNYMSAGTVEF
                     LVDRNEQFYFIEMNTRIQVEHPVTEMITGIDIVREQIRIATGEALGYRQNAVRFTGHA
                     IECRINAEDPEHFVPSPGRVTAWIAPGGPGVRVDSHLMAGYVVPPYYDSLIAKIIVHA
                     NDRGAAIARMQRALSETVVEGVKTTIPYHLKLLADPAFVAGESTLARLEQTL"
     gene            3330..3953
                     /gene="thiE"
                     /locus_tag="VNU03_16575"
     CDS             3330..3953
                     /gene="thiE"
                     /locus_tag="VNU03_16575"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWP76140.1"
                     /translation="MILDRSVAGGRQLPDLLGAVLAGGCRLVQLREKTMPLDELLALA
                     RHLARQCREADARFIVNDRADLALACGADGLHVGQDDLPAREARALLRPGMILGVSTH
                     DPEQARRAIADGADYVAVGSIFPTTTKAGFQLVGTDLIRRVRPEVSVPLVGIGGITSD
                     NASAVLGAGADGVAVISAVCAAADPEASTRRFLERIVARLSASPGAS"
     gene            3965..>5069
                     /locus_tag="VNU03_16580"
     CDS             3965..>5069
                     /locus_tag="VNU03_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76141.1"
                     /translation="MRRFLPAFIGVLSFIVFIPALSGEFLTWDDDINLVSNPFYRGLG
                     SSQIRWAFSNVRMGHYIPLTWLGLSANYAAGGMNPWGYHLVNVILHVANAVTFYFVAR
                     RLLAAAVDGGRQDRRADPALAWGAAVAALVFALHPLRVESVAWITERRDVQSGFFYLL
                     SILTHLRSLEAAEGAQWRWRAGSLLLFAGGLLSKASIMVLPAVLVLLDVYPLRRGALS
                     WRRLVIEKAGYWVLGAAGAGGALIALRLSGLQITSYGAYGPEARAAMIAYSFWFYPST
                     WVWPVRLSPMYELPARVDPLAEPFLGAIVGLVVVTALLVLFRRRWPAGLAAWLYSALL
                     ILPISGAVHAGFQLAHDRYSYLSGLGFALVAGGA"
BASE COUNT          762 a         1759 c         1750 g          798 t
ORIGIN      
        1 tcccagctga ggaacgaccc ggacagcgca gggaggaagc aggcgatggt cagcaggacc
       61 acgaaggacg ggatcagtgt ccgaagcttg gacgggggcg ttgccacacc ctcaaatctc
      121 ccatggcgac tcaccaggac caagcggaaa ggactcgcag gctatcggaa tgattgactc
      181 tgcgggccct gccgcgtata acgaatgatg gatggctaac ccggtctctc cggtcggcgg
      241 gaccctgaca cccgatgatc cagccaccct cagcgccatt cgccgctatg aggagcgcct
      301 ggcccgggat ccaacctcgc tggcgttcgc acccctggcc gatgcgtatc gcaagaccgg
      361 tcgagtccgc gacgccatcc ggctctgccg ggaagggctg gagcgctttc cgcattacgt
      421 gacggcgcga ctcatcctgt cgaaggcgct cctcgacgat ggcgatccgg acggcgcgct
      481 gagcgaggtg cgggccatcc tcgaggtgag ccctcacgag gcgcaggctc atcgcctggc
      541 cggcgatctc tatcgtcggt ccggggcgct ggccgacgcg gtcagccacc tggagcacgc
      601 ggtgcggctc gacccgggcg accgtgaatc gcgcgcgctg ctcgacgtcc tcggggcggg
      661 gggatccgtg tcggacaact cggcgctcaa gcagctgctg gccgacgata cgttcgtcac
      721 gatgagcttc ggccgggtct gcctcgagca ggggctagtc gacgaggcgg cgcagatctt
      781 cctgcgtctc ctcggtcgcg atctgcgcca cgtcggggcc cgcgagggcc tcgaggaagc
      841 gcttcgactc aagactcaac gacggaaggg gtcatgatct catggtggtg tccaccgcgg
      901 aattcaagaa gggcctgaag attcgcttcg acggcgagcc gtacagcatc gtcgacttcc
      961 agcacgtcaa gcccggcaag ggcggggcgt tcgtgcggac caagctcaag cacatgaggc
     1021 tcggccgcgt catcgacaac acgttccgcg cgggcgagaa ggtcgagctg gtcgacttcg
     1081 aggacaagcg gatgcagtac ctctaccacg acgatcgctt ccatttcatg gacctcgaca
     1141 cctacgatca gatctccctc tcggcggagg aggtcggcga ggcgcgcgac ttcctcaagg
     1201 agaacatcga ggtggagatc ctgttcatca acgacagccc gatcacagtc gagctgccga
     1261 acttcatcga gctgcagatc gccaagaccg atcccgggat ccgcggcgac accgcgtcgg
     1321 gcggctcgaa gcccgcgacc ctggagaccg gcgcggtggt ccaggtgccc ctgttcctca
     1381 acgaggggga cgtggtgaag gtggacacgc gcagcgggga atacctcggc cgcgtggcga
     1441 cggccggctg atggccgccc ggcgaccggc tcgcggcgcg gacggatccg ggcgcgtcac
     1501 cctcgaccag gtcgtcgagc tcgcggtgcg ccacaacctc gccgagatcg aggtggaggc
     1561 ggcgggcctc cgcatccgcg tcgtccggga gcacgccccc gcggcgggcg ggagccacgg
     1621 ggacgtgagc tccgccgccg cgctccccca gcccatcccg gagcgggccg agtccaccgc
     1681 ccacctcgtc ccggtggagg ccccgatggt cggcaccttc taccgcgcgc cgaagcccga
     1741 cgcacccccg ttcgtcgccg agggggacgt ggtcaaggag gggcaggtgc tctgcatcgt
     1801 cgaggcgatg aagctgatga acgagatcga ggccaaggtc tcgggacgca tcgtcaaggt
     1861 ggtggtggag aacggccagc cggtcgagtt cggtcagtcg ctgttcctga tggagcccct
     1921 gcgctgaccg atcgagcccc cgcgctgacc cgggactgat gttccacaag atcctcatcg
     1981 ccaaccgggg cgagatcgcg ctccgcatca tccgggcctg ccgcgagatg ggaatccgga
     2041 ccgtcatcgc ccactcgacc gccgacgcgg cctccctgcc ggtgcggctg gccgacgagt
     2101 cggtctgcat cgggcctccc gaggcccggg ggagctacct caacatcccg agcatcatct
     2161 cggccgccgc gatcaccgac agcgaggcca tccatcccgg ctacgggctg ctctcggaga
     2221 acccggcgtt cgcggagatc tgccgggcgt gcggcatcac cttcatcggt ccctcgccgg
     2281 aagcgatccg gctcatggga gacaaggccc aggcccgcga gatggccaaa caggcgggag
     2341 ccccggtggt gcccggcagc gaggggccgc tcggcggggt ggacgaggcc caggcgctcg
     2401 ccgactcgat gggctatccg gtgatcgtca aggccgcggc aggtggcggc gggcggggca
     2461 tgcgcatcgt ccgcgagcga gagggcctgg ctcgcgccta cgccacctgc caggcggagg
     2521 ccggagccgc gttcggctcc tccgagctgt acctggagaa gttcgtggag gaggcgcggc
     2581 acgtcgagat ccaggtgctc ggagacaaga acggcatgcg cgtgcacctg ggcgagcgcg
     2641 actgcacggt gcagcgccga caccagaagc tcctcgagga gtcgcccgcg ccggcgctcg
     2701 cgcccgagac gcgggccggg ctctacaagg cggccctcgc ggtggccaat gcggtcaact
     2761 acatgtcggc gggcacggtg gagttcctgg tggaccggaa cgagcagttc tacttcatcg
     2821 agatgaacac ccggatccag gtggagcatc cggtcaccga gatgatcacc gggatcgaca
     2881 tcgtgcggga gcagatccgc atcgccaccg gggaggcgct cggctaccgg cagaacgcgg
     2941 tgcgcttcac cggccacgcc atcgaatgcc ggatcaacgc ggaggatccg gagcacttcg
     3001 tgccctcccc cggccgcgtc accgcctgga tcgcgccggg cggccccgga gtccgggtgg
     3061 acagccacct gatggcgggc tacgtggtgc ccccgtacta cgattcgctc atcgccaaga
     3121 tcatcgtgca cgccaacgat cggggcgcgg cgatcgcccg catgcagcgg gcgctgtccg
     3181 agaccgtggt cgagggggtg aagaccacga tcccctacca cctcaagctg ctggccgacc
     3241 cggcgttcgt ggcgggcgag tccacgctgg cccggctcga gcagaccctg tagggagctc
     3301 tccccgtgcg gttgccctcc ccgctctacg tgatcctgga tcgatccgtc gccgggggtc
     3361 ggcagttgcc cgacctgctg ggcgccgtcc tcgccggtgg ctgccgtctg gtgcagctcc
     3421 gcgagaaaac catgccgctc gacgagctct tggcgctggc ccgacacctg gcgcggcaat
     3481 gtcgggaggc ggacgcgcgc ttcatcgtga acgaccgggc cgacctggcg ctcgcctgcg
     3541 gtgcggacgg actgcacgtg gggcaggacg acctccccgc ccgcgaagcc cgggcgctcc
     3601 tgcgtcccgg aatgatcctg ggggtgtcga cccacgatcc ggaacaggct cgacgcgcaa
     3661 tcgccgacgg cgcggactac gttgcggtgg gcagcatctt cccgaccacg accaaggccg
     3721 ggttccagct cgtcggcacc gatctcatcc gccgggtgcg tcccgaggtg tcggtcccgc
     3781 tcgtgggcat cggggggatc acgtccgaca acgcgtccgc ggttctcgga gccggcgccg
     3841 acggggtggc cgtcatctcg gcggtgtgcg cggcggcaga ccccgaggcg tcgactcgcc
     3901 gcttcctcga gcggatcgtc gcgcgcctga gcgcgtcgcc cggcgcctcc taggaccggc
     3961 tctggtgcgc cgattcctcc cggccttcat cggggtgctg tccttcatcg tcttcatccc
     4021 cgcgctctcg ggagagtttc tgacctggga cgacgacatc aacctggtgt cgaacccgtt
     4081 ctaccgcgga ctcggctcgt cgcagatccg ctgggcgttc agcaatgtgc gcatgggcca
     4141 ttacatcccc ctcacctggc tcgggctctc cgcgaactac gcggcgggcg ggatgaaccc
     4201 gtggggctat cacctggtca acgtcatcct ccatgtcgcc aacgcggtca cattctactt
     4261 cgtcgcgcga cggctcctcg cggcggccgt cgatggaggc cgtcaggatc ggcgcgctga
     4321 cccggccctg gcgtgggggg ccgccgtcgc ggcactcgtc ttcgccctcc atcccctccg
     4381 tgtcgagtcc gtcgcctgga tcaccgagcg gcgtgacgtt caaagtggct tcttctacct
     4441 cctctcgatc ctgacccatc tgcgaagcct ggaggccgcc gaaggggccc agtggcgctg
     4501 gcgtgcgggc tcgctgctcc tgttcgcggg aggcctgctg tcgaaggcct cgatcatggt
     4561 gctgcccgcc gtcctcgtgt tgctcgacgt ctatccgctg cggcgggggg ccctgtcgtg
     4621 gcgccggctc gtcatcgaga aagcgggcta ctgggtactg ggagccgccg gggccggcgg
     4681 tgcgctgatc gccttgcggc tctcgggcct gcagatcacc agctacgggg cgtacggccc
     4741 ggaggcccgg gccgcgatga tcgcctacag cttctggttc tacccctcca cctgggtgtg
     4801 gccggtccgg ctatcgccca tgtacgagct gccggctcgc gtcgatcccc tggccgaacc
     4861 cttcctcgga gccatcgtgg gcctcgtggt ggtcaccgcc ctgctcgtgc tcttccggag
     4921 acggtggccg gcgggactgg cggcctggct ctattccgcc ctgctgatcc tgccgatcag
     4981 cggcgcggtg catgccggct tccagctcgc gcacgaccgc tacagctacc tttcgggcct
     5041 cggcttcgcg ctcgtagcgg gcggggcga
//
LOCUS       DATMCV010000724         3215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_36283, whole genome shotgun sequence.
ACCESSION   DATMCV010000724 DATMCV010000000
VERSION     DATMCV010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36283"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VNU03_16585"
     CDS             <1..609
                     /locus_tag="VNU03_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWP76142.1"
                     /translation="RTSNAGQCLFTGIVHPERATRLARTLLGPDSFSGWGVRTLSAGE
                     ARYNPMSYHNGSVWPHDNALIAHGLARYGLGDLALQIWTGQFEAGMYFDLHRVPELFC
                     GFPQDPGEGPITYPVACAPQAWSAASVFLLFQACLGLTIDGLERRVYLTRPRLPASLD
                     ELRIHNLEVGDATVDLLLRRHEHDVGVNVLRADGDVQLMVVM"
     gene            762..1934
                     /locus_tag="VNU03_16590"
     CDS             762..1934
                     /locus_tag="VNU03_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWP76143.1"
                     /translation="MAFGGGALLAALTLDLVGEAMRKHDFYPLAVGCLLGCVAFVGLN
                     QIVNARGGFLRSFATTSEYLAKLKSAHVRSLAERLSSVPMFRVLPPDLIADLLPQVQE
                     RTYQPGTTIIRQGEPGDGLFVIERGEVDFIDERNGGRKMRTLYDHDVFGEMALLTGEP
                     RSATAVAVKETSVWSIFKEQFDRLLHASPELAEAVHGLATQRGAVLPGEAAEDRRKTA
                     AWVHAATAGLDTTLAAPTNSEVREAAARHAGAPLAIWLGILLDGIPESLVIGSSLLQA
                     TMSASLIAGLFLSNFPEALSSSVGMVHQRYSRMRIFLLWTSLMLMTGLGAYFGAILFE
                     GMSHSWFALTEGVAAGAMLTMISETMLPEAERSGGAITGASTLLGFLAAIFFKTFE"
     gene            1965..>3215
                     /locus_tag="VNU03_16595"
     CDS             1965..>3215
                     /locus_tag="VNU03_16595"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWP76144.1"
                     /translation="MSAASLAQYGAFLLMVTLLVRPVGGYLARVFGGERTWLDPVMHP
                     LERAIYRLAGVDAAAEMGWKGYAGCFVGFGLGGTLLLYALLRVQPLLHTFDPAYRPGP
                     LAPDLAMNTAISFATTTTWQAYGGETTMSYLSQVAGLTAQNFLAGAAGLAVGIAFIRG
                     LARERTPLLGNFWVDVTRAILWVLLPLALMTAPLLVWQGVPMNFSPYAHAAVLQPTAY
                     DEPVTDPDGKPVLDENGQPKTKKARLIEQIIALGPVAALEPIKNLGTNGGGFFNVNAA
                     HPFENPTPLANLLGLLSIAVLPASLTYTFGRMTGRPAQGWVLYAVMLVLFAAGLLVCH
                     MAEQRGHPLAAQGVDHVTSAFQAGGNMEGKEVRFGIGGSVLAAITTSNGATGSYNSMH
                     DSYTPIGGLVTIVNMLFGEVVFGGL"
BASE COUNT          467 a         1144 c         1043 g          561 t
ORIGIN      
        1 cgcacgtcaa acgcgggcca gtgcctcttc acgggtatcg tccaccctga gcgggccacc
       61 cgcctggccc gcacgctctt gggacccgac tcgttctccg ggtggggggt ccgcaccctc
      121 tccgccggcg aggcccgcta caatccgatg agctatcaca acggctcggt ctggccacac
      181 gataacgcgc tcatcgccca cgggctggcc cgctacggcc tgggtgacct tgcgctccag
      241 atctggactg gccagttcga ggccgggatg tacttcgacc tccaccgtgt ccccgagctg
      301 ttctgcggct tcccgcagga ccccggggag gggccgatca cgtacccggt cgcgtgcgcc
      361 ccccaggcgt ggtcggccgc gtcggtgttt cttctcttcc aggcctgcct gggcttgacg
      421 atcgatggac tggaacgccg ggtgtacctg acccggccgc gcctgcccgc gtcactcgac
      481 gagctgagaa tccacaactt ggaagtcggc gacgcgaccg tcgacctgct gctgcgccgc
      541 cacgagcacg atgtcggggt caacgtgctg cgcgctgacg gcgatgtcca gctcatggtc
      601 gtgatgtagc atcccttcct atgttgatgc tcactgatct cttgcccgcg ggggcgcccg
      661 cccaggcact catcttcggg ttgatcagcg cggcgtcgct accgctcggc gccctcgttg
      721 cgctcttctg gatccctcgg cctcgcgtca ccgcggcctt gatggccttc ggcggtggcg
      781 cgctcctggc cgctctcacg ctcgatctgg tcggcgaagc gatgcgcaag cacgatttct
      841 acccactggc ggtgggttgc ttgctcggct gtgtcgcctt cgtcgggctc aaccagatcg
      901 tgaacgcgcg gggcggcttc ctgcgcagct tcgccaccac gagcgagtat ctggccaagc
      961 tcaagagcgc gcacgtgcgg tcgctcgccg agcggctcag ctcggtaccc atgttccgcg
     1021 tattgccccc ggatctgatc gccgacctcc tcccccaagt ccaggaacgg acgtaccagc
     1081 cgggaaccac gatcatccgc cagggcgagc cgggcgacgg gctgttcgtc atcgaacgcg
     1141 gtgaggtcga cttcatcgac gagcggaacg gcggccgaaa gatgcggacg ctctacgatc
     1201 acgacgtgtt cggtgagatg gcattgctca ccggcgagcc gcgatcggcg accgcggtgg
     1261 cggtgaaaga aacctccgtg tggagcatct tcaaggagca gttcgaccgg ctcctgcatg
     1321 catcgcccga gcttgccgag gccgtccacg gcctcgccac ccagcgtggc gccgtgctcc
     1381 cgggcgaggc ggcggaggac cgacggaaga ccgcggcctg ggtgcacgcc gccaccgccg
     1441 gcctcgacac cacattggcc gcgcccacga actcggaagt ccgcgaagcg gccgcccgtc
     1501 acgcgggcgc cccgctcgcg atctggctgg ggatcctcct cgacgggatt cccgagtctc
     1561 tcgtgatcgg ctccagcctg ctgcaggcga cgatgagcgc ctcgctgatc gcgggattgt
     1621 tcctgtcgaa cttccccgag gccctctcca gctcggtggg aatggtccac cagcgctatt
     1681 ctcggatgcg gatcttcctc ctgtggacgt ccctgatgct catgacgggt ctgggagcat
     1741 atttcggcgc catcctcttc gaggggatgt cacactcgtg gttcgctctg acggagggcg
     1801 tcgccgcggg ggcgatgctg accatgatct cggagacgat gctgcccgag gccgagcgct
     1861 cgggtggggc gatcacgggg gcctcgaccc tgctcgggtt cctcgccgcc attttcttca
     1921 agactttcga gtaaccgcga cgtccggccc gaggagaaaa gggcatgagc gcagcgagtc
     1981 tcgcgcagta cggcgcattc ctgctgatgg tcaccctgct cgtcaggccg gtcggcggct
     2041 acctcgcccg ggtcttcggc ggcgagcgga cctggctcga cccggtgatg catccgctcg
     2101 agcgggccat ctaccggctc gccggggtcg acgccgccgc cgagatgggc tggaagggct
     2161 atgccggatg cttcgtcgga ttcggcctgg gcggcacgct gctgctatac gcgctgctac
     2221 gagtgcagcc gctgctccac acgttcgacc cggcgtaccg gccggggccg ctcgctcccg
     2281 acctcgcgat gaacaccgcg atcagcttcg cgaccacgac gacctggcag gcgtacggcg
     2341 gcgagaccac catgagctac ctgagccagg tcgccggcct cacggcccag aacttcctgg
     2401 ccggggccgc cggcctcgcg gtgggcatcg cgttcatccg aggcctggcc cgcgagcgca
     2461 cccctctgct tggcaatttc tgggtagacg tgacccgcgc gattctctgg gtgctgctgc
     2521 ccctcgcgct gatgaccgcc ccgttgctcg tatggcaggg cgtgcccatg aacttctccc
     2581 cctacgcaca cgccgcggtc ctgcagccga ccgcctacga cgagccggtg accgaccccg
     2641 acggcaagcc cgtcctcgac gagaatgggc agccgaagac gaagaaggcg cgcctcatcg
     2701 agcagatcat cgcgctgggc ccggtcgccg ccctcgagcc aatcaagaac ctcggcacca
     2761 acggcggagg cttcttcaac gtcaacgcgg cgcacccgtt cgagaatccc acgcccctcg
     2821 cgaatcttct cgggctgctg tccatcgcgg tcctgccggc ctcactcacc tacaccttcg
     2881 ggcgcatgac cggccggccc gcgcagggct gggtgctgta cgcggtgatg ctcgtgctct
     2941 tcgcggcggg cctcctggtc tgccatatgg ccgagcagcg cggccacccc ctggccgccc
     3001 agggggtgga tcacgtgacg agtgccttcc aggccggggg caacatggag ggcaaggagg
     3061 tccgcttcgg aatcggcggc tcggtcctcg ccgcgatcac cacgtcgaac ggcgcgaccg
     3121 ggtcctacaa ctcgatgcac gacagctaca cccccatcgg cggcctggtg acgatcgtga
     3181 acatgctttt cggtgaggtc gtgttcggcg gcctc
//
LOCUS       DATMCV010000725         3016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_638319, whole genome shotgun sequence.
ACCESSION   DATMCV010000725 DATMCV010000000
VERSION     DATMCV010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638319"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1142)
                     /locus_tag="VNU03_16600"
     CDS             complement(105..1142)
                     /locus_tag="VNU03_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWP76145.1"
                     /translation="MAQPPVSSESQEQRVAEFVSAYQSLRAEVGKIIVGHSEVVTQVL
                     IGLFAGGHVLLEGVPGLGKTLLIKTLAESLELSFSRIQFTPDLMPGDIIGTNIIVEDA
                     QGRKHFQFQRGPIFGHILLADEVNRATPKTQSALLEGMQEGAVTVSGSAHPLPAPFFV
                     LATQNPIEMEGTYPLPEAQLDRFLFKVRVRYPALEELTEIIDRTTHVRTVEVRRVLTG
                     PQVLSMRELVREIPIATHVRDLASFIVLGTHPQWERAPESARRYIRYGASPRGAQALV
                     LGAKVRALSDGRFNVSVDDLRAMALPALRHRLILNFEGEAAGVDVDDLVGQIVESAEA
                     MSGTGKEVFLR"
     gene            complement(1371..2396)
                     /locus_tag="VNU03_16605"
     CDS             complement(1371..2396)
                     /locus_tag="VNU03_16605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP76146.1"
                     /translation="MTPFAAPSVRGNAITVDRIARGLRERGVELRVWDLSVAPETAVE
                     HQIGQFRPAVVHAFHALRTGPAALRLARRVDVPLLVTVTGTDVNQDLLDPDSAPVVRR
                     VLEGAAAISVFDESMATAIVEALPELAGRLVVIPQSAAFEPTPIGHWAPAATLQRAPG
                     PLVLFPAGIRGVKRPLLPLDPLEGLLAEYPGLELIYVGPILDPAAGEGLLRTVASYPW
                     ARHIGAVPHHRMPALLELSDVVLNCSLSEGGMANSVLEALALGRAVLASDIPGNRSLV
                     EDGVTGLLFGTPEELAAKADRLLKDPDLRARLGQAGRERVSARFGRDRETDGYVAAYT
                     RLTPTVT"
     gene            2558..2821
                     /locus_tag="VNU03_16610"
     CDS             2558..2821
                     /locus_tag="VNU03_16610"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWP76147.1"
                     /translation="MDGSSREIGCSECVELLADYIDGTLPRDQTELLEWHLDGCPPCV
                     AFVNTYRGTVDAARRLRETPESKLPPELRQKLIAFLKRSDAAR"
     gene            complement(2818..>3016)
                     /locus_tag="VNU03_16615"
     CDS             complement(2818..>3016)
                     /locus_tag="VNU03_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWP76148.1"
                     /translation="RGQLRIDPNVPEALVKLLCESETSGGLLFAVEAGRAGTVRDGFA
                     RRGEAVWEVGVVTAEAELALV"
BASE COUNT          477 a         1087 c         1047 g          405 t
ORIGIN      
        1 cccgcgcagg ccgtagaact ccgcccgccg cagccggatc tgccgggcga cgtccgcgac
       61 cagccgctcg atcaccgtgg gaccgggcat cgactacctc ctctctacct caggaacacc
      121 tctttccccg tcccactcat cgcctcggcg gattcgacga tctggcccac gaggtcgtcc
      181 acgtccaccc ccgccgcctc accttcgaaa ttcaagatga ggcggtgacg cagcgcgggc
      241 agcgccatcg cccgcagatc gtcgacgctg acgttgaacc ggccgtcgga cagggcgcgg
      301 accttcgccc ccagcaccag cgcctgcgcg ccgcgcgggc tcgccccgta gcggatgtag
      361 cgacgggccg actcgggcgc ccgctcccac tggggatggg tgccgaggac gatgaacgag
      421 gcgagatcgc gcacgtgggt ggcgatcggg atctcgcgca ccagctcgcg catcgagagc
      481 acctgcggcc cggtcaggac gcgccgcacc tcgaccgtgc gcacgtgggt ggtgcggtcg
      541 atgatctcgg tcagctcctc gagggccgga taccgcaccc ggaccttgaa caggaaccgg
      601 tcgagctggg cctcgggcag cgggtacgtg ccctccatct cgatggggtt ctgggtggcg
      661 agcacgaaga acggcgccgg cagcggatgc gcggagccgg acacggtcac cgcgccttcc
      721 tgcatgccct cgagcagcgc cgactgcgtc ttgggcgtgg cccggttcac ctcgtcggcg
      781 agcaggatgt gcccgaagat gggcccgcgc tggaactgga agtgcttccg cccctgcgcg
      841 tcctccacga tgatattggt gccgatgatg tcgcccggca tcagatccgg ggtgaactgg
      901 atgcgcgaga acgacagctc gaggctctcg gcgagcgtct tgatcagaag ggtcttgccg
      961 agacccggca cgccttcgag gagcacgtgg ccgcccgcga agagaccgat caggacctgg
     1021 gtgacgacct cgctgtggcc gacgatgatc ttgccgacct cggcccggag cgactggtaa
     1081 gcgctgacga actccgcgac ccgctgctcc tgggactcgc tcgacacggg aggctgcgcc
     1141 atgaacggct cctttcccgc cttggaattg cctcatcata ggggggcgtc ccacccccgt
     1201 caactacttg cccccgatgc cgtcgcgcgt ctgctctcgc gcttgccctc cgcgtgtgcg
     1261 acgtgccgaa aattcactca acgcgtcgcg cgaagagcgc tcgaaacccc atcggcgggc
     1321 gccccgcgcg cgctcgagcg cgagcgaggc aaggagcgtg ccgcgagcgc ctaggtgacc
     1381 gtgggtgtca gccgcgtgta cgcggcgaca tagccgtccg tctcgcggtc gcggccgaag
     1441 cgcgcgctca cgcgctcccg cccggcctga ccgagtcggg cgcggagatc gggatccttg
     1501 agcaggcgat cggccttggc ggccagctcc tcgggcgtgc cgaacagcag cccggtgacc
     1561 ccgtcctcga cgagcgagcg gttgcccggg atgtcggagg cgaggaccgc gcggccgagc
     1621 gccagcgcct cgagcaccga gttggccatg ccgccctcgg agagcgagca gttgagcacc
     1681 acgtcggaca gctcgagcag ggccggcatg cggtgatgcg gcaccgcgcc gatgtggcgc
     1741 gcccacgggt aggacgcgac cgtccgcagc aggccctctc ccgcggccgg atcgaggatc
     1801 ggcccgacgt agatcagctc gaggcccgga tactccgcga gcagcccctc gagcggatcg
     1861 aggggcagga gtggacgctt cacgccccgg atcccggccg ggaacagcac gagcgggccg
     1921 ggcgcccgct gcagcgtcgc ggcgggagcc cagtgcccga tgggagtcgg ctcgaaggca
     1981 gcgctctggg gaatcaccac gaggcgaccg gccagctccg gcagggcctc cacgatcgcg
     2041 gtggccatcg actcgtcgaa gacgctgatc gcggccgcgc cctcgagcac ccggcgcacg
     2101 acgggggccg agtccggatc gaggaggtcc tggttgacgt cggtcccggt gacggtcacg
     2161 agcaggggca cgtccacgcg acgggccagg cggagcgcgg ccggaccggt gcggagggcg
     2221 tgaaaggcgt gcacgaccgc gggccggaac tggccgatct ggtgctcgac cgcggtctcc
     2281 ggcgccaccg acagatccca gacgcgcagc tcgaccccgc gctcccgaag cccgcgggcg
     2341 atgcggtcga ccgtgatcgc gttacctcga accgagggag cggcgaacgg cgtcaagata
     2401 gccacgcggg cggccatggg tgcgacgagc ctacactggg gccgcgcggg aaggcaatgc
     2461 gggcggggac cccgcgggca aagaccgact tgcctcggcg cgggcgtgtg ttaaggtgac
     2521 gtcacaagcc cagtgcctca tcggtgaggg agagcgaatg gacgggtcct cgcgggaaat
     2581 cgggtgctcg gagtgtgttg agctcctcgc cgactacatc gacggcactc ttccacgaga
     2641 ccagaccgag ctgctggaat ggcacctgga tgggtgcccg ccgtgcgtgg cgttcgtcaa
     2701 cacctaccga ggcaccgtcg acgccgcccg ccgcctccgt gagacccccg aatccaagct
     2761 gccccccgag ctgcgccaga agctgatcgc cttcctgaag cgctccgacg ccgcgcgcta
     2821 gaccagagcc agctccgcct ccgccgtcac caccccgact tcccacaccg cctcgccgcg
     2881 ccgcgcgaag ccgtcccgca ccgtcccggc ccgtccggcc tccaccgcga agagcagccc
     2941 gcccgacgtc tccgactcgc agagcagctt gaccagggcc tcgggcacat tcgggtcgat
     3001 gcgcaattgt ccccgg
//
LOCUS       DATMCV010000726         3948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_173725, whole genome shotgun sequence.
ACCESSION   DATMCV010000726 DATMCV010000000
VERSION     DATMCV010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173725"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VNU03_16620"
     CDS             <1..429
                     /locus_tag="VNU03_16620"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76149.1"
                     /translation="PSADWDALRALGEGIGAELARRAPDRYTINPLKAARRGRIFVDY
                     LRNVRGATSVAAYSPRARADAPVSTPLGWSELAGGATPGDFTVQTVPRRLAARKDPWA
                     DFFSVDQAITARTARALAPPAAPRRSPPPRGRARTRPARS"
     gene            complement(332..910)
                     /locus_tag="VNU03_16625"
     CDS             complement(332..910)
                     /locus_tag="VNU03_16625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP76150.1"
                     /translation="MFGTAPSDFALEVARLVRPGDYVLDLGCGEGRDSVFFAERGAVV
                     FGVDLSEAGLAKARRLARARGVRVRWVHGPATGLLPTGPFDLIFSCGSLHYVARDQRA
                     DLFARLCAMTRPGGRHAHLVFTEDLVHEEKGEIVEYYRAGELREAFVNWQVLKQADHV
                     ISCAQDGSVHGHAVEEIVAARPVGPAPWPSEP"
     gene            1184..2329
                     /locus_tag="VNU03_16630"
     CDS             1184..2329
                     /locus_tag="VNU03_16630"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWP76151.1"
                     /translation="MALIDKREFVGLDGVAHLCTGGEAPWLRAHTTACERFGALKSGG
                     MAGREEMFAIGERARARAARVLGVAPAELAFLAHSSEGLNQAVRSVEWRAGDNVVFAD
                     LEYPSSIYPAAMLRERGVEARVVRTRAHYLSLDDLAALVDRRTRLILVSQVSYLTGQR
                     LDLARCADIAHGVGARLAVDATHAAGVVPVPGEVCDFVVSSCYKWLLATHGVGLFGYS
                     ARRVGELVPATVGWHSVGHRGGPDDPLAMSWRADASRLEAGNPSLLGLAVLDSALARL
                     EQLAPDAVERHAVGLGGRLIEGLRSRGFTVLTPEAPAERAGNVCFLVDDAAGLAGRLA
                     GLRVLVWGGEGRIRVSPHVHCDGDDVERFFEALDRVGARPGQAVAAH"
     gene            2438..3340
                     /locus_tag="VNU03_16635"
     CDS             2438..3340
                     /locus_tag="VNU03_16635"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HWP76152.1"
                     /translation="MEALLSLSNDLAGAVERAAPSIVAVHARRRLPSTGIHWRPGIVV
                     TADHTVRMEDGITVETADGRARPAALVGRDPGSDLAVLRVADLDTEPAALGDDGALRV
                     GHMVLALGYGPRASWGVISALGPRWRTWRGGEIDRLVRLDLVLYPGFSGGPLVDATGR
                     VIGLNTSGLARESRLALPVATVARVTDELLRKGHVARAYLGIGMQPVPLPEPLRAALG
                     LPGDGGLIVVMVEPDGPAARAGLLLGDVLVALDGAPTGDLEDVQAQLGGERIGTTVTA
                     LVVRAGARVEVRITVGERPRRGAS"
     gene            3337..3924
                     /locus_tag="VNU03_16640"
     CDS             3337..3924
                     /locus_tag="VNU03_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWP76153.1"
                     /translation="MSAVLTAEPTLEPLAAAAAAMAAELARVTVEVRGRGRGAGGAAV
                     GAGVIWHPDGLILTNAHVAHHDVTVVLADGRARGARLVARDPRRDLAALVVDATGLPT
                     AEIGDSAALRVGELVLAVGNPLGLVRALSAGLVHAVGPRAIHADLRLAPGNSGGPLAD
                     ARGRVVGLNAMIVGGLAVAVPSREAQRFVREQLRG"
BASE COUNT          451 a         1444 c         1535 g          518 t
ORIGIN      
        1 ccctcggccg actgggacgc gctccgggcg ctcggcgagg ggatcggggc ggagctggcc
       61 cggcgcgcgc ccgatcgcta cacgatcaac cccctgaagg cagcgcgccg cgggcggatc
      121 ttcgtcgact acctccgcaa cgttcgaggg gcgacctcgg tggcggccta ctcgccccgc
      181 gcgcgggcgg atgccccggt gtcgacgccg ctcggctgga gcgagctggc gggcggagcg
      241 acccccgggg acttcacggt gcagacggtg ccgcgccggc tggccgcgcg gaaggacccg
      301 tgggccgact tcttctcggt ggatcaggcg atcacggctc ggacggccag ggcgctggcc
      361 ccaccggccg cgccgcgacg atctcctcca ccgcgtggcc gtgcacggac ccgtcctgcg
      421 cgcagctgat cacgtggtcg gcctgcttga gcacctgcca gttcacgaac gcctcgcgta
      481 gctcgcccgc ccggtagtat tccacgatct ctcccttctc ctcgtgcacc aggtcctcgg
      541 tgaagacgag atgcgcgtgg cggccgcccg gccgggtcat cgcgcacagc ctcgcgaaga
      601 gatcggcgcg ctggtcgcgg gccacgtagt gcaggctgcc gcacgagaag atcaggtcga
      661 agggcccggt cggcagcagg ccggtcgcag gcccgtgcac ccagcggacc cggaccccgc
      721 gcgctcgcgc caggcgccgc gccttggcga ggcccgcttc cgagagatcc accccgaaca
      781 ccaccgcgcc ccgctccgcg aagaagacgc tgtcgcggcc ttccccgcag ccgaggtcga
      841 gcacgtagtc gcccggccgt acgagccggg cgacctcgag cgcgaagtcc gatggagcgg
      901 tgccgaacac gtagcgctcg ggagtgcgcg catactctcg cgcccaggga ctccaggtcg
      961 tcttcgaccg ttgcgatgcg ctcggcatcg gtaaccccgc acgcaagccg ggtgccagcg
     1021 ccggggcggg cgcggctcgt cgcagggcgc gagaaacgcg tgatttccgc gccagcggca
     1081 gggcggcgag cgggccgccg gcgggacgcg ggtaagcctt accgggtagt tcgacgcctc
     1141 gcccgatgct aggctacatc cggtcaaccc gaggaggacc accgtggcgc tcatcgacaa
     1201 gcgggagttc gtgggactgg acggcgtggc gcatctctgc accggcgggg aggcgccctg
     1261 gctccgggcg cacaccacgg cctgcgagcg gttcggggcg ctgaagagcg gcggcatggc
     1321 cggccgcgag gagatgttcg cgatcgggga gcgggcgcgg gcccgggccg cccgggtgct
     1381 gggggtcgcg ccggcggagc tggccttcct ggcgcactcc tccgagggcc tgaaccaggc
     1441 ggtgcgctcg gtggagtggc gcgccggcga caacgtggtc ttcgccgatc tcgagtaccc
     1501 gtcgtcgatc tacccggccg cgatgctgcg cgagcggggg gtcgaggccc gcgtggtgcg
     1561 cacccgcgcc cactatctct cgctcgacga cctcgcggcc ctggtcgacc ggcgcacccg
     1621 cctgatcctg gtgagccagg tgagctatct gaccgggcag cggctcgatc tggcgcgctg
     1681 cgccgacatc gcccacggcg tcggggcccg gctggccgtg gacgctaccc acgcggccgg
     1741 agtggtgccg gtgccgggcg aggtctgcga cttcgtggtc tcgagctgct acaagtggct
     1801 gctcgccacc catggggtcg gcctcttcgg atacagcgcc cgccgcgtcg gggagctggt
     1861 gccggccacc gtgggctggc acagcgtcgg gcaccggggc ggccccgacg atccgctcgc
     1921 catgtcgtgg cgcgccgacg cctcgcggct cgaggcggga aacccgagcc tgctcggcct
     1981 cgcggtgctg gacagcgcgc tcgcgcgcct ggaacagctg gctcccgatg cggtggagcg
     2041 ccacgcggtc ggcctgggcg ggcggctgat cgaggggctc cggtcgcgcg gcttcacggt
     2101 cctcacgccg gaggcgcccg cggagcgcgc cggcaacgtc tgcttcctgg tcgacgacgc
     2161 ggccgggctc gcgggacggc tcgccggcct gcgagtgctg gtctggggcg gcgagggccg
     2221 catccgcgtc tcgccgcacg tgcactgcga cggcgacgac gtcgagcggt tcttcgaggc
     2281 gctcgaccgg gtcggcgcgc gccccggcca ggcggtggcg gcgcactagc tcacctggcc
     2341 atccggccgg gtcccgacct ggccattcgg ctgatgtccg cggttcactc ctccggcatg
     2401 ctgagggcgc accctcactc gtaaggagga cacacgaatg gaagccctgc tctcgctctc
     2461 gaacgatctg gccggcgcgg tggagcgcgc ggccccgtcc atcgtcgcgg tgcacgcgcg
     2521 ccggcgcctg ccgtccaccg ggatccactg gcgccccggc atcgtggtca ccgccgatca
     2581 cacggtccgg atggaggacg gcatcaccgt cgagaccgcg gacggccgcg cgcggcccgc
     2641 cgccctcgtc ggtcgcgatc ccggctcgga cctcgcggtg ctgcgcgtgg cggatctcga
     2701 cacggagccg gccgcgctcg gggacgacgg cgcgctgcgc gtcggccaca tggtgctcgc
     2761 gctcggctac gggccccgcg cgagctgggg cgtgatcagc gcgctcggcc cgcgctggcg
     2821 cacctggcgc ggcggtgaga tcgatcggct ggtgcgcctg gacctcgtgc tctacccggg
     2881 cttctcgggc gggccgctgg tcgacgcgac cgggcgcgtg atcggcctga acacctccgg
     2941 cctcgcgcgc gagagccggc tcgcgctgcc ggtggccacg gtcgcgcggg tgaccgatga
     3001 gctgctccgc aagggccatg tcgcgcgcgc ctacctgggc atcggcatgc aaccggtccc
     3061 gctgccggag cccctccgcg ctgcgctcgg cctgcccggc gacggcgggc tcatcgtggt
     3121 gatggtggag cccgacgggc ccgcggcgcg cgcgggcctg ctcctgggcg acgtgctggt
     3181 ggcgctcgac ggcgcgccga ccggcgatct ggaggatgtc caggcgcagc tcggcggcga
     3241 acgcatcggc accacggtca ccgcgctggt ggtacgcgcg ggggcgcgcg tggaggtgcg
     3301 catcacggtg ggcgagcgcc cgcggcgggg cgcatcgtga gcgccgtcct gaccgccgaa
     3361 cccaccctcg agccgctcgc cgcggccgcc gccgcgatgg ccgcggagct ggcccgcgtc
     3421 acggtggagg tgcgcgggcg ggggcgcggc gccggcggag cggcggtggg cgcgggcgtg
     3481 atctggcacc cggacggcct gatcctcacc aacgcgcacg tggctcacca cgacgtgacg
     3541 gtggtgctgg ccgatggccg ggcgcggggc gcgcggctgg tcgcgcgcga cccccggcgc
     3601 gacctggccg cgctggtggt ggacgcgacc ggactcccga ccgcggagat cggcgactcg
     3661 gccgcgctgc gggtcggtga gctggtgctg gcggtcggca acccgctcgg gctggtgcgc
     3721 gcgctctcgg ccggcctcgt gcacgcggtc gggccgcggg cgattcacgc cgacctgcgc
     3781 ctggcccccg gcaactcggg cggcccgctc gccgacgcgc ggggccgcgt ggtcgggctc
     3841 aacgcgatga tcgtgggcgg cctcgcggtg gcggtgccga gccgcgaggc gcagcgcttc
     3901 gtccgggagc agctgcgagg ctgagggcgt gaccacggtg ctggtggc
//
LOCUS       DATMCV010000727         5067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_173765, whole genome shotgun sequence.
ACCESSION   DATMCV010000727 DATMCV010000000
VERSION     DATMCV010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173765"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VNU03_16645"
     CDS             <1..827
                     /locus_tag="VNU03_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76154.1"
                     /translation="LLVAAFADVIAPRGYDDQDLAFALQPPAWAGGTWANPLGTDPLG
                     RDVLSRVIYGARTSLLLALGSVLLAAAIGVTLGLVSGWAGGRVDAAIMSAVEAQLSLP
                     YLLFAIAFMALLGSSFTNLVIVLVLRSWVVYAQLVRVSVLSMKTREFVTVAVALGARG
                     RRILFRHIAPNVIAPALVVSSFQLAELIIVESSLGFLGLGVQPPTPSWGSMLSQGREY
                     LGSAWWLVTFPGLAIIVTVLGANLFGDALRDALDPRLRTRARGARGLFRRTFKETS"
     gene            830..1864
                     /locus_tag="VNU03_16650"
     CDS             830..1864
                     /locus_tag="VNU03_16650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP76155.1"
                     /translation="MDFGLFVPCHRFDASLSVKELYDQVLEMVKLADEAGFNTVWFPE
                     HHLVQYYASPSPLMWMVKAAAATRRVRVGAAILVIPYYNPLRLAGEIGMADVLCDGRL
                     EIGVGRGAFEYEFDRFGIDEKIGGARLREGMEILEGLLTQDDFSYEGTTCSFAPATAV
                     PRSLQQPHPPIWVAARSPDTIAWALKRGYNLLTTPWRDPFSRIQKHHEQVMQIAAAVG
                     VTKPPKIAVSRMTYVGATDGDALEAMADIQVSHRIFMRLFRNEATVKGGFTLPEPVED
                     EYSREQLMANLVAGSPATCVEKVRDYEQLGISQFILYGAFGPDHSRTMASLRRFAEQV
                     MPHFQKGSRS"
     gene            1861..3201
                     /locus_tag="VNU03_16655"
     CDS             1861..3201
                     /locus_tag="VNU03_16655"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76156.1"
                     /translation="MTSHPGSSVTLISAERLVDGTGREPLARGTVAVQGDRIVAVGER
                     AELAARFPGAARADFPGATIVPGMVDSHVHVTFSASAVPFEDIQNDGDVTVGLRGAIN
                     ARDALLAGVTTLRDLGGRQRTTLELRNAINAGAIPGPRLLVCGRPVTIPNGHCHFLGG
                     VAQGVPEVTRLTGELVEQGADVIKVMATGGNMTVTSDPLKAQFTKEEMRAIVGIANQA
                     GLRVTAHARGVEGIRVAAEANAHGIEHCRMEVGTGEWQFDHELARMLADKGIFAAPTL
                     AASYRAFQRQAAGGKVGIRAGAIPFPVRQKNAAMLREAGIRVVVGTDAGASLCRFDEA
                     VHVEMEALVGAGWTPVEAIHAGTLGSATAIGREKDLGSLEAGKLADVVVVRGNPAQSI
                     SDVRQVEAVYLNGRRVASRGHATLDERPTPWPQSAIATRTEYRGTDVGPRLPKA"
     gene            3212..4129
                     /locus_tag="VNU03_16660"
     CDS             3212..4129
                     /locus_tag="VNU03_16660"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWP76157.1"
                     /translation="MAKPRLLNMNGRVIPYEDARVHVLSTAMKYAASVYEAMRAYWSD
                     ADQQLYVFRLREHLRRLERSAKIARIPLPADAARLERETLEIIRANDLREDLHVRLIV
                     FVDTDDGGLASREPVGYVIAPIPTTRYFETGGLHVAVSSWTRTADNAIPARVKAAANY
                     QNSRLALLQAKTDGYDDAILLNGDGSVAEGPGYTLFLVRDEQPMTPSVTSNILEGVTR
                     STLLSLFPEALGLTVEQRQIDRTELYTADEAFFTGTAAEVTPILSIDRRPVGDGTTGP
                     LTAKVRDVYFRAVRDGSAPHPEWRLPVWV"
     gene            4136..>5067
                     /locus_tag="VNU03_16665"
     CDS             4136..>5067
                     /locus_tag="VNU03_16665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP76158.1"
                     /translation="MPAASADVVIVGGGVMGTSIALQLARRRAGRIVLCEKRHVASGP
                     TGRSSALLRRHYSIELYCRMAARSFELYSNFEELTGRPAEITRCGLVMAVGPEDVAAL
                     RDTVTMMRGLGVKVEELAPRDLARVVPGVAVDDFGAAAWETDTGYGDPAGVSQAYAAR
                     ARELGVEIRQETTVSGFTLTGGRISGVRTSGGDIATSTVVNAAGPWGAALSRAVGVDL
                     PVVASRQQLVILRPRAEATAARPVLVDMVERAYYRPETGGLVLVGTRNRAGVSAAVDP
                     DAFSERVDADRVAWTAEAAARRFPGFADAEALGG"
BASE COUNT          677 a         1888 c         1807 g          695 t
ORIGIN      
        1 cgctcctggt ggcggcgttc gcagatgtca tcgccccgcg cggctacgac gaccaggacc
       61 tggccttcgc gctccagccg ccggcctggg cggggggcac gtgggccaac ccgctcggca
      121 ccgacccgct cggccgcgac gtcctgagcc gggtgatcta cggcgcgcgc acctcgctcc
      181 tgctcgcgct cggctcggtg ctgctggccg ccgccatcgg cgtgacgctc gggctggtca
      241 gtggctgggc cggcggccgg gtggacgcgg ccatcatgag cgcggtggag gcgcagctct
      301 cgctgcccta cctgctcttc gccatcgcgt tcatggcgct gctgggctcg agcttcacga
      361 acctcgtgat cgtcctggtg ctgcgcagct gggtggtcta cgcccagctg gtgcgcgtct
      421 cggtgctgtc gatgaagacg cgcgagttcg tcacggtggc ggtggcgctc ggcgcgcgcg
      481 gccgccgcat cctgttccgc cacatcgccc ccaacgtgat cgcccccgcc ctggtggtgt
      541 cgagcttcca gctcgcggag ctgatcatcg tggagtcgtc gctcggcttc ctcggcctgg
      601 gcgtgcagcc gcccactccc agctggggca gcatgctgag ccagggccgc gagtacctgg
      661 gctcggcgtg gtggctggtc acgttccccg ggctggccat catcgtcacc gtgctgggcg
      721 ccaatctctt cggcgacgcc ctgcgcgacg cgctggatcc ccggctccgc acccgcgccc
      781 gcggcgcgcg cggcctgttc cgccggacct tcaaggagac ctcctgacca tggacttcgg
      841 cctcttcgtt ccctgccatc gcttcgacgc ctcgctgagc gtcaaggagc tgtacgacca
      901 ggtgctcgag atggtgaagc tcgccgacga ggccggcttc aacacggtgt ggttccccga
      961 gcaccacctg gtgcagtact acgcctcgcc ctcgccgctc atgtggatgg tgaaggcggc
     1021 cgccgcgacc cgccgcgtgc gggtgggcgc cgcgatcctg gtgatcccct actacaaccc
     1081 gctgcgcctg gccggcgaga tcggcatggc cgacgtgctg tgcgacgggc gcctggagat
     1141 cggcgtgggc cgcggggcct tcgagtacga gttcgaccgc ttcggcatcg acgagaagat
     1201 cggcggcgcc cgcctccgcg agggcatgga gatcctggag ggcctgctca cccaggacga
     1261 cttctcctat gagggcacca cctgctcctt cgcgcccgcc accgcggtgc ccaggtcgct
     1321 ccagcagccc cacccgccca tctgggtggc ggcccgctcc cccgacacca tcgcctgggc
     1381 gctcaagcgc ggctacaacc tgctcaccac cccgtggcgc gatccgttct cgcgcatcca
     1441 gaagcaccac gagcaggtga tgcagatcgc ggccgcggtc ggggtgacga agccgcccaa
     1501 gatcgcggtc tcccgcatga cctacgtggg ggccaccgac ggggacgcgc tcgaggcgat
     1561 ggccgacatc caggtcagcc accgcatctt catgcgcctc ttccgcaacg aggccaccgt
     1621 caagggcggc ttcaccctgc ccgagccggt ggaggacgag tacagccgcg agcagctcat
     1681 ggccaacctg gtggcggggt cgcccgcgac ctgcgtggag aaggtccgcg actacgagca
     1741 gctcggcatc agccagttca tcctctacgg ggccttcggg cccgaccact cgcgcaccat
     1801 ggcctcgctc cggcgcttcg cggagcaggt gatgccgcac ttccagaaag ggtcccgctc
     1861 atgaccagtc atcccggcag ctccgtcacc ctgatctccg ccgagcgcct cgtcgacggc
     1921 accggccgcg agccgctggc ccgcggcacg gtcgcggtgc agggcgaccg catcgtggcg
     1981 gtgggcgagc gcgccgagct ggccgcccgc ttccccggcg cggcgcgggc cgacttcccc
     2041 ggcgccacca tcgtgccggg catggtggac tcccacgtgc acgtcacctt cagcgcgagc
     2101 gcggtgccgt tcgaggacat ccagaacgac ggtgacgtga cggtggggct gcgcggcgcc
     2161 atcaacgcgc gcgacgccct gctggccggg gtcaccactc tccgcgacct cggcgggcgg
     2221 cagcgtacca ccctcgagct gcgcaacgcg atcaacgcgg gcgccattcc cggcccgcgc
     2281 ctgctggtgt gcggccggcc ggtcacgatc ccgaacgggc actgccactt cctgggcggc
     2341 gtggcccagg gcgtgcccga ggtcacccgc ctcaccggcg agctggtgga gcagggcgcc
     2401 gacgtcatca aggtgatggc caccggcggc aacatgacgg tgacctccga cccgctcaag
     2461 gcccagttca ccaaggagga gatgcgcgcg atcgtgggga tcgcgaacca ggccggcctg
     2521 cgggtcaccg cgcacgcgcg cggcgtggag ggcatccggg tcgccgcgga ggccaacgcg
     2581 cacggcatcg agcactgccg catggaggtg ggcaccggcg agtggcaatt cgaccacgag
     2641 ctcgcccgca tgctggccga caagggcatc ttcgcggcgc cgacgctggc ggccagctac
     2701 cgcgccttcc agcgccaggc cgccggcggc aaggtcggca tccgcgccgg cgcgatcccg
     2761 ttcccggtgc gacagaagaa cgcggcgatg ctccgcgagg ccggcatccg ggtcgtggtg
     2821 ggcaccgacg cgggggcgtc cctctgccgc ttcgacgagg cggtgcacgt ggagatggag
     2881 gccctcgtcg gcgcgggctg gacgccggtg gaggccatcc acgccggcac cctgggctcc
     2941 gccaccgcga tcggccggga gaaggacctg ggcagcctcg aggcgggcaa gctcgcggac
     3001 gtggtggtgg tgcgcggcaa cccggcccag tcgatcagcg acgtgcgcca ggtggaggcg
     3061 gtctacctga acggccggcg ggtggcgagc cgaggccacg ccaccctcga cgagcggccg
     3121 accccgtggc cgcagagcgc catcgccacc cgcaccgagt accgcggcac cgacgtcggc
     3181 ccgcgcctgc cgaaggcctg aggagccgac catggccaag ccccgcctgc tcaacatgaa
     3241 cggccgcgtg atcccctacg aggacgcgcg cgtccacgtg ctctccaccg cgatgaagta
     3301 cgcggccagc gtctacgagg cgatgcgcgc ctactggagc gacgcggacc agcagctcta
     3361 cgtcttccgc ctccgcgagc acctgcgccg gctggagcgc tcggccaaga tcgcccgcat
     3421 ccccctgccc gcggacgcgg cccgactcga gcgggagacg ctcgagatca ttcgcgccaa
     3481 cgacctccgc gaggacctgc acgtccgcct gatcgtcttc gtcgacaccg acgacggcgg
     3541 gctcgcctcg cgggagccgg tgggctacgt gatcgcgccc atcccgacca cgcgctactt
     3601 cgagaccggg ggcctccacg tggcggtcag ctcctggacc cgcaccgccg acaacgcgat
     3661 cccggcgcgg gtcaaggcgg cggccaacta ccagaacagc cgcctcgcgc tgctccaggc
     3721 caagaccgac ggctacgacg acgcgatcct gctcaacggc gacggcagcg tggccgaggg
     3781 gccgggctac acgctcttcc tggtgcgcga cgagcagccg atgaccccgt cggtgacgag
     3841 caacatcctg gagggcgtca cccgctcgac tctcctgagc ctcttcccgg aggcgctcgg
     3901 gctcacggtg gagcagcggc agatcgaccg caccgagctc tacaccgcgg acgaggcgtt
     3961 cttcaccggc accgcggccg aggtcacccc gatcctgtcg atcgaccggc gccccgtcgg
     4021 cgacggcacg acgggcccgc tcaccgccaa ggtgcgcgac gtctacttcc gtgcggtccg
     4081 cgacggctcg gcgccgcacc cggagtggcg ccttccggtc tgggtgtagc cgccgatgcc
     4141 cgccgcgtcg gccgacgtcg tcatcgtggg cggcggcgtg atgggcacct ccatcgcgct
     4201 gcagctggcc cggcggcgcg ccggccggat cgtgctgtgc gagaagcgcc acgtggcctc
     4261 cggccccacc ggccggtcga gcgcgctgct gcgccgccac tactcgatcg agctctactg
     4321 ccggatggcg gcgcgctcgt tcgagctcta ctccaacttc gaggagctga ccggccgccc
     4381 ggccgagatc acccgctgcg ggctggtgat ggcggtgggc cccgaggacg tggcggcgct
     4441 gcgcgacacc gtcaccatga tgcgcgggct cggggtgaag gtggaggagc tcgccccgcg
     4501 cgacctggcg cgcgtggtgc ccggcgtcgc ggtggacgac ttcggcgccg cggcctggga
     4561 gaccgacacc ggctacggcg atccggccgg cgtgagccag gcctacgcgg cgcgggcacg
     4621 cgagctgggc gtggagatcc gccaggagac gacagtgtcg ggcttcacgc tgaccggggg
     4681 gcggatctcg ggtgtgcgca ccagcggcgg tgacatcgcc acctcgacgg tggtgaacgc
     4741 ggccgggccc tggggcgccg cgctctcgcg cgcggtcggc gtcgacctgc cggtggtggc
     4801 gagccggcag cagctcgtga tcctgcgccc gcgcgcggag gccaccgccg cgcggccggt
     4861 gctggtggac atggtggagc gcgcgtacta ccggcccgag accggcggcc tcgtgctggt
     4921 cggcacccgc aaccgcgccg gggtgagcgc ggcggtcgat cccgacgcgt tctccgagcg
     4981 ggtcgacgcc gaccgcgtgg cgtggaccgc ggaggcggcc gcccggcgct tccccggctt
     5041 cgcggacgcg gaggcgctcg gcggcta
//
LOCUS       DATMCV010000728         2611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_374907, whole genome shotgun sequence.
ACCESSION   DATMCV010000728 DATMCV010000000
VERSION     DATMCV010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374907"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..385)
                     /locus_tag="VNU03_16670"
     CDS             complement(125..385)
                     /locus_tag="VNU03_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76159.1"
                     /translation="MRPYVLLVLAIFASVLLAGLLGRLWRRDEQRAVERKWQERERLL
                     DRRRPVATRREVLRRRLRPTRPTYRGRRSAAPSRGGRRARPG"
     gene            464..1381
                     /locus_tag="VNU03_16675"
     CDS             464..1381
                     /locus_tag="VNU03_16675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP76160.1"
                     /translation="MKLGFSLPTAGAWATPENQIRVARQAETLGYHSLWVFQRLLYAL
                     DPKNEYPPVPGKTWPKPFERVLDPIVTLAYVAAVTSRIRLGTSVLIMPFYAPVVLAKQ
                     LATLDVLSGGRLDVGLGLGWSEDEYEAVGVPYKARGRRGDEFLRCLDMIWTQDVIEFH
                     GEFYHVPRCRVEPKPVQKPRPPITIGGYGSTVVKRAVELADGFNGGNVPMASVAPLVR
                     EVREAAAAHGKDPARLHIVCRGSYRVLDTPQGPDRRPLWGTLDEIREDIDRYAAAGLT
                     ELFLEGNFTPGGPSLEQTLEVMTRLAPAG"
     gene            1669..1887
                     /locus_tag="VNU03_16680"
     CDS             1669..1887
                     /locus_tag="VNU03_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76161.1"
                     /translation="MAGRSGRIGLVVALLGLMLPLPAWAGSAQATLTVGVVVPARCAV
                     RVASSLAASDGMGGAREAVAMRCTKGNF"
     gene            2088..>2611
                     /locus_tag="VNU03_16685"
     CDS             2088..>2611
                     /locus_tag="VNU03_16685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP76162.1"
                     /translation="MDDSLPSILCVDDDVDVLDLLKEYFTEQGFVVLTATNGVEAFLQ
                     VKRWQPKAVILDLFMPRLGGIGALGRIKALNPDVVVILVSGMGNALELVTEAGLSVAG
                     AFAKPLDLMQLSQTLARAGVVAPSAFVRGETSPERRPVRARILVVDDELEFRKVLAEY
                     LEDRGFDVIEAQAG"
BASE COUNT          392 a          905 c          892 g          422 t
ORIGIN      
        1 cccgtcgctc cggtcgggac ggacaggagc gcggcgatca tcggcagagc cagtcgccgg
       61 gtgcgcgcca tccgcctcat catacccggc gggaccagag agaccggggc cgggcgctgc
      121 cggatcagcc cggtcgggcg cgtcggcctc cgcgagaggg tgcggcgctc cgccgccccc
      181 ggtacgtggg ccgggtcggc cgcaggcgcc gcctcaggac ctcccgacgg gtcgcgaccg
      241 ggcgccggcg gtccagcagg cgctcccgct cctgccactt ccgctcgacg gctcgctgct
      301 cgtcccgcct ccacaggcgt ccgagcaacc cggcgagcag gacgctcgcg aagatggcca
      361 ggacgagcag gacgtagggt ctcacgggag ccgaagcgta ccagagcgcg ggtgacctgc
      421 caagccgagc gtggctcgag ggccgcgtgt atcatgtcgc ccgatgaagc tcggattctc
      481 gctgcccacc gccggggcgt gggccacccc ggagaatcaa atccgcgtcg cgcgccaggc
      541 cgagacgctc ggctaccact cgttgtgggt gttccagcgg ttgctctacg cgctcgatcc
      601 gaagaacgag tacccgccgg tgccgggcaa gacgtggccc aagccgttcg agcgcgtgct
      661 cgaccccatc gtcacgctcg cctacgtggc cgcggtcacc tcgcgtatcc gcctcggcac
      721 gagcgtgctg atcatgccgt tctatgcccc ggtggtcctg gccaagcaac tcgccactct
      781 cgacgtgctc tcgggggggc ggctcgacgt cgggctgggc ctcggctggt cggaggacga
      841 gtacgaggcg gtcggggtgc cgtacaaggc gcgcgggcgt cgcggcgacg agttcctccg
      901 ctgcctcgac atgatctgga cccaggacgt gatcgagttc cacggcgagt tctatcacgt
      961 gccgcgctgc cgggtggagc ccaagccggt gcagaagccg cgcccgccga tcaccatcgg
     1021 cggctacggc agcacggtgg tgaagcgcgc ggtcgagctg gccgacgggt tcaacggcgg
     1081 caacgtgccg atggccagcg tggccccgct ggtgcgcgag gtccgggagg cggcggccgc
     1141 gcacggcaag gatccggccc ggctccacat cgtctgccgc ggctcctacc gagtactcga
     1201 tacgccgcag ggccccgacc gtcggccgtt gtggggcacg ctcgacgaga tccgcgagga
     1261 catcgaccgc tacgcggccg ccggcctcac cgagctgttt ctcgagggca acttcacgcc
     1321 gggcgggccg tcgctcgagc agaccctcga ggtgatgaca cgcctcgccc ccgcgggcta
     1381 ggccgagcgc ggagtcgtcg ccttcttctg acccaccgcc tcgagccctc acagcgcggc
     1441 tgtcggtgct atggcggccg tcccatacga ggcggatcgg gcccatggga ttcgcgctac
     1501 ccgcgattgt cggcacccgc aggaatactc tcaactactt taaatattta tatttgtagg
     1561 ctgtcatcca gctgacactc gattcgaatt cttggccccc cgcttgccct ggcctagagt
     1621 gctgtagcaa aatccacagc cgcgaggcgg agaagataga gccagaacat ggcggggcga
     1681 agcggacgga tcgggctggt agtcgcgctg ctggggctca tgctgcccct gcccgcgtgg
     1741 gccggatccg cgcaggccac gcttaccgtc ggcgtggtcg tccccgcccg ctgcgccgtc
     1801 cgggtggcga gctcgctcgc cgccagcgac gggatgggcg gggcccggga ggcggtggcc
     1861 atgcggtgca ccaagggaaa cttctaggcc ctgaagccgt acctcccagc cagttccact
     1921 ctgttggtga ccggtagtgt cagcctcctg tcaggatctg tgaaggatct tgcaatcgcc
     1981 gcaacggctt tcgcagcaaa gcgcagttat ttcgtctggt tagcaatgac acactcggcg
     2041 tccaaatagc gatatccccg gcactgttct tgctctgacg gttcgtcatg gacgatagcc
     2101 tcccgagcat cctgtgtgtg gatgacgacg tcgacgtcct cgacttgctc aaggagtact
     2161 ttaccgagca ggggttcgtc gtcctgaccg ccaccaacgg cgtcgaggca ttcctccagg
     2221 tcaagcgctg gcaaccgaag gcggtgatcc tcgatctctt catgccccgt ctcggcggca
     2281 tcggcgccct cgggcgcatc aaggccctca atcccgacgt cgtggtgatc ctggtcagcg
     2341 gcatgggcaa cgccctcgag ctggtcaccg aggccggcct gagtgtcgcg ggcgctttcg
     2401 caaagccgct cgacctcatg cagctctccc agacgctggc ccgggcgggc gtggtggccc
     2461 cctccgcctt cgtcaggggc gagacctcgc cggagcgccg gccggtccgc gcgcgcatcc
     2521 tggtggtgga cgacgagctc gagttccgca aggtcctggc cgagtacctc gaggaccgcg
     2581 gcttcgacgt gatcgaggcg caggcgggcg a
//
LOCUS       DATMCV010000729         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_573380, whole genome shotgun sequence.
ACCESSION   DATMCV010000729 DATMCV010000000
VERSION     DATMCV010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573380"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..2173)
                     /locus_tag="VNU03_16690"
     CDS             complement(59..2173)
                     /locus_tag="VNU03_16690"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWP76163.1"
                     /translation="MRRRRHFCYKKAVTTTPALLIMLSIDGLRADYIDDPTVDLPALR
                     GLAARGARARRMTPIFPTVTWPCHTSIVTGVSPARHGVLGNLVFDRASGKPVEHYGDR
                     TDAPVRVPTLWDRLHATGQRTAAVCWPKTRGIAAVADNIPEFYEQELFEAYASRPLWT
                     ELAARRLPVDRYGPWSAQHPMGPMQDWLTLEAARHILAVRPPRLMLLHFLTLDSFQHD
                     HGVDSPEARWALLQMDALLGRLLDTLAEQGRLETTTLMVFGDHGFVNVSRRHHLNQLL
                     REEGLLDLDRHGDITQRRAWVAGNGGAAHVYVLDGAPRTTGERLAERFRALPGLDVLE
                     PARFAALGLPAPGPDSMQGDLMLAAHEGVLITGQRDARGRRGGAGAARGSRPRAVAAA
                     DGRGLRDGRARGAGRRAPRHGLHARPRADRGPSPRGGPAGGGGHAAGRGARRALKGIL
                     IAAGRGQRLMPLTEDVPKCYAEIGGRRMLDWCVDALGHAGCGDIVFVGGYRVGRVQAD
                     YPRFRFVHNAEWERNNILLSLFSAEHEMAGGFVSSYADILYTSEAAARLVRHPGDIVL
                     LVDTDWRARYRPRTQHPESDGEKVRLYHDRVTEVSRRIPPAEAPAEFTGVAKFTAAGA
                     RLLVQHFHRARERFDGRAFGNSAVFARAYLIDLLQAMLEAGVTLHAVETPGGYFEVDT
                     TEDHGLASRDWDASRRRAGSAR"
BASE COUNT          327 a          846 c          901 g          307 t
ORIGIN      
        1 cgcacgaagt ccgagcgcgg catgctgccg accaccgagg acgggaagcg gggcagggtc
       61 accgggcgct cccggcccgc cggcgcgagg cgtcccagtc gcgcgaggcg agtccgtggt
      121 cctcggtggt gtccacctcg aagtagccac ccggcgtctc caccgcgtgc agggtcaccc
      181 ccgcctcgag catcgcctgg agcaggtcga tcaggtaggc gcgcgcgaag acggccgagt
      241 tgccgaaggc gcggccgtcg aagcgctccc gcgcgcggtg gaagtgctgg acgaggagtc
      301 gcgcgcccgc ggcggtgaac ttggccaccc cggtgaactc ggccggcgcc tccgcgggcg
      361 ggatgcggcg gctcacctcg gtcacccggt cgtggtagag gcgcaccttc tcgccgtcgg
      421 actcggggtg ctgggtgcgc ggccggtagc gcgcgcgcca gtcggtgtcg accagcagca
      481 cgatgtcgcc cgggtgccgc accaggcgcg cggccgcctc cgaggtatag aggatgtcgg
      541 cgtagctcga gacgaagccg cccgccatct cgtgctcggc gctgaagagc gagagcagga
      601 tgttgttccg ctcccactcc gcgttgtgca cgaagcggaa gcgcgggtag tcggcctgca
      661 cgcgccccac gcggtagccg cccacgaaga cgatgtcccc gcagcccgcg tggccgagcg
      721 cgtccacgca ccagtcgagc atgcgccggc cgccgatctc ggcgtagcac ttcgggacgt
      781 cctcggtgag gggcatgagc cgctggcccc ggcccgccgc gatcaggatg cccttcaacg
      841 cgcggcgagc gcctcgacca gcggcgtgcc ctccgccccc ggcaggtcca ccgcgaggag
      901 acgggccgcg gtcggcgcga ggtcgagcat ggagaccgtg tcgaggcgcg cgccttcccg
      961 caccccgggc ccggccatca cgaagcccgc gcccatcagc ggcagcgacg gcgcgtggcc
     1021 gtgagccgcg cgcagcaccg gcgccgccgc ggcggcctcg ggcgtcgcgt tggccggtga
     1081 tgagcacgcc ctcgtgggcg gccagcatca ggtcgccctg catggagtcg gggccgggcg
     1141 cgggcaggcc gagagcggcg aagcgcgcgg gctcgagcac gtcgagcccg gggagcgcac
     1201 ggaagcgctc cgcgagccgc tccccggtgg tgcgcggcgc gccgtcgagc acgtagacgt
     1261 gtgctgcgcc cccgttgccc gcgacccacg cgcgccgctg cgtgatgtcc ccgtgccggt
     1321 ccagatcgag cagcccctcc tcgcgcagga gctggttcag gtgatgtcgg cgggagacgt
     1381 tcacgaaccc gtggtcgccg aagaccatca gggtggtggt ctcgaggcgg ccctgctcgg
     1441 ccagggtgtc gagcagtcgc ccgagcagcg cgtccatctg caggagcgcc cagcgcgcct
     1501 ccgggctgtc caccccgtga tcgtgctgga aggaatcgag ggtgaggaag tgcaggagca
     1561 tcaggcgcgg cgggcggacc gccaggatgt gacgcgccgc ctccagggtg agccagtcct
     1621 gcatcggccc catcgggtgc tgtgcgctcc agggcccgta gcgatccacc ggcaggcgcc
     1681 tggcggccag ctcggtccag agcgggcgcg aggcgtaggc ctcgaacagc tcctgctcgt
     1741 agaactcggg gatgttgtcg gccaccgcgg cgatcccgcg cgtcttgggc cagcacaccg
     1801 ccgcggtgcg ctggccggtc gcatgcaggc ggtcccacag ggtcggcacc cgcaccggcg
     1861 cgtcggtgcg gtcgccgtag tgctcgaccg gcttcccgct cgcgcggtcg aagaccaggt
     1921 tgccgagcac gccgtgccgc gcgggcgaga cgccggtgac gatcgaggtg tgacacggcc
     1981 acgtgacggt ggggaagatc ggcgtcatcc gtcgggcgcg cgcgccacgc gcggcgaggc
     2041 cgcggagcgc gggcaagtcc acggtgggat cgtcgatgta gtcggcgcgg agcccgtcga
     2101 tgctcagcat gatcagcagg gcgggcgtcg tcgtcaccgc cttcttgtag cagaagtgac
     2161 gccgccgtcg caagcgcgct tcccgtataa taaccgggct ccatgtccac ctccgtcctg
     2221 gtcggcgtcg atatcggcac gtcatccgcc aagggggcag cctacgatcc ggacggacgc
     2281 gcgctcggca ccgtcgagat cgcgtatcgc cacgagatgc cgcgccgcgg atgggccgag
     2341 gccgacgcga ccgcgtggtg gcgggcgacg tgtcgcatcc t
//
LOCUS       DATMCV010000730         5469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_908403, whole genome shotgun sequence.
ACCESSION   DATMCV010000730 DATMCV010000000
VERSION     DATMCV010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908403"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VNU03_16695"
     CDS             <1..201
                     /locus_tag="VNU03_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2236 domain-containing protein"
                     /protein_id="HWP76164.1"
                     /translation="LWLAALPAVGLLPLSIRAGYGLPWDARRARALALVAAATRVSLP
                     LVPPPLRYWGAARRAMRRARRG"
     gene            198..659
                     /locus_tag="VNU03_16700"
     CDS             198..659
                     /locus_tag="VNU03_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP76165.1"
                     /translation="MSNPAITLAVESPRQAEVTRLLEALDAYQGALYPPESNHFLDVE
                     ALAAPNMRFIVARRGAEVVGCGALRIDGACGELKRMYVVPEARGQRLGRRILDRIEVE
                     ARREGLGCLRLETGIYQPEALGLYRSAGYAEREAFAPYAPDPLSVFMEKIL"
     gene            complement(587..955)
                     /locus_tag="VNU03_16705"
     CDS             complement(587..955)
                     /locus_tag="VNU03_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76166.1"
                     /translation="MTLQSLEMRGRGIGWKADRCADPETCDVHLCVVDDEGGYWHPAD
                     PLAFAKWRVRAELDAMPGHVVVKVGRGWDALRRGAVVGAVGLGGTAAVLGMWWGRQRI
                     FSMKTLSGSGAYGAKASRSA"
     gene            1362..1997
                     /gene="tmk"
                     /locus_tag="VNU03_16710"
     CDS             1362..1997
                     /gene="tmk"
                     /locus_tag="VNU03_16710"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWP76167.1"
                     /translation="MRGALITVEGVEGSGKSTQCARLAAHLTARGIEVVLTSEPDGTP
                     LGARIRSLFEAGPTPTPLTQTFLFMAARQEHVTRVIAPALTRGAVVISDRYTDATVAY
                     QGYGQGVDVQTIGELNMLATGGLLPDLTLVLDLDPVVGMRRIRDRALDAFEKMDLAFH
                     RRVREGYLEIAHSDKNRVVVLDAGREPEVLHADVVRSVDECLARRGGGRGA"
     gene            1987..2874
                     /locus_tag="VNU03_16715"
     CDS             1987..2874
                     /locus_tag="VNU03_16715"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HWP76168.1"
                     /translation="MALEGIRDQDEAVTLLRRALATGRVAHAYAFVGPEGSGRKATAL
                     ALAAALVAPDGGSAAERAARGRHPDVHLLGPTPPAGNPKGAQALRVETIRELERLASL
                     KPMEARVKVFIVDEAEKMTAATPQAFLKTLEEPPAQTVIVLILTQLRALPPTVLSRCQ
                     VVRFRPRATEGVPALLPDVRSEVHTRSLRQLAEARAQGVEAIIRLGEQVGRDRQAAEA
                     FVQACWLWHRDLLCGLGGAPARLAVFGEPAIAAARERSLDSVLRALRDCREAWQAIQG
                     NVSPRLSVEVLVGRLVEAA"
     gene            2879..3778
                     /gene="ricT"
                     /locus_tag="VNU03_16720"
     CDS             2879..3778
                     /gene="ricT"
                     /locus_tag="VNU03_16720"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HWP76169.1"
                     /translation="MNEPIEEGAPGALESPVVDETPRYLIGVRLREPLLADDYQTTDA
                     DLHVGDFVLVETGGAPVLGEVRRPKRPVPSFKRDRVYRRVVRRATRGEVNEWRERRAL
                     EVKGVDTSARMARARGLAMKVVDVEIDASRRRVTVFFNSEERVDFRDLVRDLAREFHA
                     RIEMRQIGARDTTKLLDGIGPCGRQLCCSSHLRKFDPISVKMAKAQDMPLTDSRLLGN
                     CGRLKCCLLYEFSTYEELRSRLPRVNSACSASCGGGGCMTGKVRSLRVLKQSVVVGFP
                     DGTEAEVPLDQLTWEGRDHIKPT"
     gene            3798..>5469
                     /gene="metG"
                     /locus_tag="VNU03_16725"
     CDS             3798..>5469
                     /gene="metG"
                     /locus_tag="VNU03_16725"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWP76170.1"
                     /translation="MSTFYLTTPIYYVNARPHLGHACTTIMADAMCRYRRLRGDRVFF
                     LTGTDEHGERIAQVAAAAGKSPQAYADEIAAVFRETWRRLGITNDDFIRTTEPRHKKV
                     VQEVLQRLYDAGEIYFGEYGGWYCYGCERFYTEKEIVDGKCPDHQTALTFIKEKNYFF
                     KMSKYQQWLIKHLEDNPSFIQPDRYRNEVLGFLREPLQDLSISRPTSRLEWGIPLPFD
                     DKYVTYVWFDALLNYYSAVASGGEKTRGLWEHVEHLIGKDILKPHGVYWPTMLKAAGI
                     PLYRHLHVHGYWSLGGSKMSKSVGNVVEAFQLTDKYGHDAFRYFLLREMNFGLDANFN
                     EEALVDRFNSDLANDLGNLVSRATTMIVNFGGGIIPAVGGPGDPEPAVFNLPAAWQQT
                     LDAVDVAMNEFAFQKALTALWAFIRVVNRYIDELQPWALAKSGRAEDGTRLKDVLFML
                     GASLRCLGIVLWPFIPDAAAKILAAVGETDPPRLSEAKLSSLAAGARVQKAPALFPRI
                     EVKVADKVSVVDSAKLEVRSSKIGIADFQKVELRVAEVLAAERVPKSKK"
BASE COUNT          840 a         1861 c         1920 g          848 t
ORIGIN      
        1 ctctggctcg ctgccctgcc cgccgtcggg ctgctgccgc tgtcgatccg cgcgggctac
       61 gggctgccgt gggatgcccg gcgcgcgcgg gccctggcgc tggtggcggc ggccacgcgc
      121 gtgagcctgc cgctcgtgcc tccgccgctg cgctactggg gggcggcgcg tcgcgcgatg
      181 cggagagcgc ggcgcggatg agcaaccccg ccatcaccct cgcggtggag tcgccccggc
      241 aggccgaggt cacgcgcctg ctcgaggccc tcgacgccta tcagggcgcg ctctacccgc
      301 cggagagcaa ccacttcctc gacgtggagg cgctcgccgc gccgaacatg cggttcatcg
      361 tggcgcgccg cggagcggag gtggtgggct gcggtgcgct tcgcatcgac ggggcctgcg
      421 gagagctgaa gcggatgtac gtggtgccgg aggcgcgggg ccagcggctg gggcgacgca
      481 tcctggaccg catcgaggtg gaggcgcgcc gcgagggact gggctgcctg cgcctggaga
      541 ccggcatcta ccagccggag gcgctcggtc tgtaccggtc ggcgggctac gcggagcgcg
      601 aggccttcgc gccgtacgcg ccggacccgc tcagcgtctt catggagaag atcctctgac
      661 ggccccacca catgcccagt accgccgcgg tgccgccgag gccgacggcg cccaccaccg
      721 cgccgcgccg gagcgcatcc cagccacggc cgaccttgac cacgacgtgc ccgggcatcg
      781 cgtcgagctc ggcgcggacg cgccacttcg cgaaggccag gggatccgcc ggatgccagt
      841 agccgccctc gtcgtcgacg acgcagaggt gcacgtcgca cgtctccgga tcggcgcagc
      901 ggtcggcttt ccagccgatg ccgcggccgc gcatctcgag gctctgaagg gtcacgcgag
      961 cagccatatc gcacccattc cgaccagcac cgccgtcctg atcaatcccg ccaggcccat
     1021 ggtcacgcgc ctcctcttct cgctcctgct catgtgacgc gcccggtgcg tccagcgttt
     1081 cgctacgcgt gcgtgacgct ggacccgaga gcggaggcgg gttgacccct cgcgcagaac
     1141 cggtgtgtgg cgaggggtcc gtgtgccgac ccgctctccg cctcgggcac tcatggagca
     1201 gagcaagacg cgagccacga gggaaataga gcaaattccc gaaacgaagt cggttcgctg
     1261 ctctgcgtta ggaaaggtcg ttacagtact gtggaacgat tttccctgag gtggccagtg
     1321 gcgtggcgac gcctcccgaa atcggtgtta gagtacgctg catgcgcggg gcgctgatca
     1381 ccgtcgaggg ggtggagggg tctggtaaat ccacccagtg cgcgcggttg gcggcccatc
     1441 tgacggcccg cggcatcgag gtcgtcctga cgagcgagcc cgacggcacc cccctgggtg
     1501 cccggatccg cagcctcttc gaggccgggc cgaccccgac ccccctgacc cagacgttcc
     1561 tcttcatggc cgcccggcag gagcacgtga cccgggtgat cgccccggct ttgacgcggg
     1621 gcgccgtggt gatctccgac cgctataccg acgccaccgt cgcctaccag ggctacgggc
     1681 aaggggtgga cgtccagacg atcggcgagc tgaacatgct cgcgacgggc ggcctcctgc
     1741 ccgacctcac tctcgtgctc gacctggacc cggtggtggg catgcggcgc atccgcgacc
     1801 gcgccctcga cgccttcgag aagatggatc tggcgttcca ccgccgggtg cgggagggct
     1861 acctcgagat cgcccacagc gacaagaacc gggtggtggt gctggacgcc ggtcgggagc
     1921 cggaggtcct gcatgccgac gtggtccgct cggtggacga gtgcctggcg cggcgaggtg
     1981 gtggtcgtgg cgcttgaggg tatccgggac caggacgagg cggtgaccct gctgcgccgg
     2041 gctctggcca ccgggcgcgt ggcccacgcc tatgccttcg tcgggcccga gggctcgggt
     2101 cgcaaggcga ccgcgctggc cctcgcggcg gcgctggtgg ccccggacgg gggatcggcg
     2161 gccgagcggg ccgcgcgggg ccgccatccc gacgtgcacc tgctggggcc gacgccgccg
     2221 gcgggcaatc ccaagggagc gcaggcgctc cgcgtggaga cgatccggga gctcgagcgg
     2281 ctcgcctcgc tcaagccgat ggaggcgcgc gtcaaggtct tcatcgtcga cgaggccgag
     2341 aagatgaccg cggccacccc gcaggcgttc ctcaagacgc tcgaggagcc gccggcccag
     2401 accgtgatcg tcctgatcct gacccagctc cgcgcgcttc cgcccacggt gctgtcgcgc
     2461 tgtcaggtgg tgcgcttccg cccccgggcc accgagggcg tgccggcgct cctgcccgac
     2521 gtgcggagcg aggtccacac ccggagcctg cgccagctcg cggaagcccg ggcgcagggc
     2581 gtggaagcca tcatccggct cggcgagcag gtgggccgcg accggcaggc cgccgaggcc
     2641 ttcgtccagg cgtgctggct ctggcaccgc gatctgctgt gcggcctcgg cggcgccccc
     2701 gcgcgcctgg ccgtcttcgg cgagccggcc atcgcggcgg cgcgcgagcg ctcgctcgac
     2761 tcggtcctgc gcgcgctccg cgattgccgc gaggcgtggc aggccatcca ggggaacgtg
     2821 tcgccgcgac tgagcgtcga ggtgctcgtg gggcgcctcg tggaggcggc ctgaagccat
     2881 gaacgagccg atcgaggagg gcgctccggg agcgctcgag tcgcccgtcg tcgacgagac
     2941 gccccgctac ctgatcgggg tgcgcctgcg cgagccgctg ctggccgacg actaccagac
     3001 caccgatgcg gacctgcacg tgggcgactt cgtgctcgtg gagaccggcg gggcgcccgt
     3061 gctgggcgag gtgcgccggc ccaagcggcc ggtcccgtcg ttcaagcgcg accgcgtcta
     3121 tcgccgggtc gtgcgccggg ccacccgggg cgaggtgaac gagtggcgcg agcgccgggc
     3181 cctcgaggtg aagggggtgg acaccagcgc gcggatggcg cgggcccgcg ggctcgccat
     3241 gaaggtggtc gacgtcgaga tcgacgcctc ccgccgccgc gtgaccgtct tcttcaactc
     3301 cgaggagcgg gtggacttcc gcgacctggt gcgcgacctg gcccgcgagt tccacgcccg
     3361 gatcgagatg cggcagatcg gggcccgcga caccaccaag ctgctcgacg gcatcggtcc
     3421 ctgcggccgc cagctctgct gctcgtcgca cctgcgcaag ttcgacccga tctcggtgaa
     3481 gatggcgaag gcgcaggaca tgccgctcac cgacagccgg ctcctcggca actgcgggcg
     3541 gctcaagtgc tgtctcctgt acgagttctc gacctacgag gagctgcgct cgcggctgcc
     3601 gcgggtgaac tcggcgtgct cggcctcgtg cgggggcggc ggctgcatga ccggcaaggt
     3661 gcgctcgctg cgggtgctga agcaatcggt ggtggtcggg ttccccgacg gcaccgaggc
     3721 ggaggtgccc ctcgatcagc tcacgtggga ggggcgcgac cacatcaagc cgacctgagg
     3781 gcccagtcac ccgacgcatg agcaccttct acctcacgac cccgatctac tacgtcaacg
     3841 cccggccgca cctcgggcac gcctgcacca ccatcatggc cgacgcgatg tgccgctatc
     3901 gccggctccg cggcgatcgc gtgttcttcc tcaccggcac cgacgagcac ggcgagcgga
     3961 tcgcccaggt cgcggcggcg gccgggaagt caccgcaggc ctacgcggac gagatcgcgg
     4021 ccgtcttccg ggagacctgg cggcggctcg gcatcacaaa cgacgacttc atccgcacca
     4081 ccgagccgcg gcacaagaag gtcgtgcagg aggtcctgca gcggctctac gacgcgggcg
     4141 agatctactt cggcgagtac ggcggctggt actgctacgg ctgcgagcgc ttctacaccg
     4201 agaaggagat cgtggacggc aagtgccccg atcaccagac cgcgctcacc ttcatcaagg
     4261 agaagaacta cttcttcaag atgtcgaagt accagcagtg gctgatcaag cacctcgagg
     4321 acaatccgag cttcatccag ccggaccggt accggaacga ggtgctgggc tttctccgcg
     4381 agccgctgca agacctgtcg atcagccggc ccacctcgcg gctcgagtgg ggcatcccgc
     4441 tgccgttcga cgacaagtac gtgacgtacg tctggttcga cgcgctcctc aactactact
     4501 ccgcggtcgc gagcggcggc gagaagacgc gcggcctgtg ggagcacgtc gagcacctga
     4561 tcggcaagga catcctgaag ccccacgggg tctactggcc gaccatgctg aaggcggccg
     4621 gcatcccgct ctaccggcac ctccacgtgc acggctactg gtcgctcggg ggcagcaaga
     4681 tgtccaagtc ggtcggcaac gtggtggagg ccttccagct caccgacaag tacggccacg
     4741 atgccttccg ctacttcctg ctgcgcgaga tgaacttcgg gctcgacgcc aactttaacg
     4801 aggaagcact ggtcgatcga tttaactctg atctcgcgaa cgacctaggt aatctcgtga
     4861 gccgagctac cactatgata gtgaatttcg gtgggggcat tatccctgcg gttggtgggc
     4921 ccggggaccc tgaacctgcg gtcttcaatc ttcctgcggc atggcagcag acgttggacg
     4981 cagtcgacgt tgcgatgaac gagttcgctt tccagaaggc cctgaccgct ttgtgggcgt
     5041 tcattagagt agtgaatcgc tatattgatg aactacaacc ctgggctctt gctaagagtg
     5101 gtcgagcgga ggatgggacc cgactcaagg atgtcctctt catgctcggt gctagtctta
     5161 gatgccttgg aatcgtgttg tggcccttca tccctgatgc ggcagcaaag atccttgccg
     5221 cggtgggaga gacagatcca ccgcggctct cggaagcgaa gctttcctca ctagctgctg
     5281 gtgcgagggt ccagaaagca cctgccctat ttcctaggat cgaggtgaaa gttgcagaca
     5341 aggtgtcggt cgtagactcc gcaaagcttg aagtgcgcag ttctaagatt gggatcgccg
     5401 acttccagaa ggtcgagctg cgggtcgccg aggtgctggc cgccgagcgc gtgccgaagt
     5461 ccaagaagc
//
LOCUS       DATMCV010000731         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_638494, whole genome shotgun sequence.
ACCESSION   DATMCV010000731 DATMCV010000000
VERSION     DATMCV010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638494"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VNU03_16730"
     CDS             complement(<1..1041)
                     /locus_tag="VNU03_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWP76171.1"
                     /translation="MLFTLASEFAPSGDQPEAIDRLVSMVGEGSPHTVLLGVTGSGKT
                     FTLANVMARLGRPALVISPNKTLAAQLYSEFKAFFPGNAVEYFVSYYDYYQPEAYIPQ
                     SDTYIEKDALVNDEIDRMRHSATASLFERRDVVVVASVSCIYGIGEPETYHGMHVALR
                     AGQTMDRDTLIRSLVAVQYERNDYDFRRGTFRVRGDVVEVFPAAAESEALRVEFWGDT
                     VERLMTLEPLRGVTTGSVPEVRVYPASHYVTPAAQLERAIEGIMDELRERLGYLKARG
                     KLLEAQRLEQRTLFDMEMLRELGYCHGVENYSRHLSGRKPGQNPPTLMDYLPKDALVI
                     LDESHVTAPQLRG"
     gene            complement(1062..1592)
                     /locus_tag="VNU03_16735"
     CDS             complement(1062..1592)
                     /locus_tag="VNU03_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWP76172.1"
                     /translation="MDEIAVLVLNYTYEPLHFTNAKRAITLLLAGKAESVEASPRVIR
                     SPSRSFHLPAVIRLGAYIRKPFLERVAFNKKNILRRDGYTCQYCSRRGEKLTVDHVVP
                     RSRGGQTTWTNVVAACLRCNLLKGNRTLEEARLRLIREPVHPQFLFSVHLLRHPHATS
                     FLDSWRKYLVAVPSTS"
     gene            complement(1642..2076)
                     /locus_tag="VNU03_16740"
     CDS             complement(1642..2076)
                     /locus_tag="VNU03_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76173.1"
                     /translation="MSLIPRVVVRRWLETLLALFSLAVLYVNQHREAMPRALDLKDDT
                     NLSLLDWLFRGVVFGMMGVLGFSALVVIFFLVYSPIYLINKIPHLVGKGGWLDKREVR
                     FYLACFALICLLISLFAWSSEAFTMLLVLLAGFGPLVWRLLV"
     gene            complement(2139..>2624)
                     /locus_tag="VNU03_16745"
     CDS             complement(2139..>2624)
                     /locus_tag="VNU03_16745"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HWP76174.1"
                     /translation="FYALVEAAACGLTLEPKEAARLVEVGERIRLAIEARVPLVHPAM
                     PDARGLLYVQFHEPARRADAHLRNAVVVAPAGLDRSPCGTGTSARLANLHARGRLGVG
                     EGFGHESIIGSLFTGRIAGLTQVAGTAAVVPEITGRAWMTGRSTLTLDPSDPFPGGFL
                     L"
BASE COUNT          438 a          877 c          946 g          363 t
ORIGIN      
        1 cccgcgcagc tggggcgcgg tgacgtgcga ctcgtcgagg atcaccagcg cgtccttggg
       61 gaggtagtcc atcagggtgg gcgggttctg gcccggcttg cgccccgaga ggtggcgcga
      121 gtagttctcg accccgtggc agtagccgag ctcgcgcagc atctccatgt cgaacagggt
      181 gcgctgctcg agccgctgcg cctccaggag cttgccgcgc gccttgaggt acccgagccg
      241 ctcgcgcagc tcgtccatga tgccctcgat cgcccgctcg agctgggccg cgggggtcac
      301 gtagtggctc gccgggtaga cgcggacctc gggcacgctg ccggtggtca cgccccggag
      361 cggctcgagg gtcatcaggc gctccaccgt gtcgccccag aactcgaccc gcagggcctc
      421 cgactcggcc gcggccggga acacctcgac cacgtcgccg cgcacccgga aggtcccgcg
      481 ccggaaatcg tagtcgttgc gctcgtactg caccgcgacc agcgagcgga tcagggtgtc
      541 gcggtccatg gtctggcccg cccgcagggc cacgtgcatg ccgtggtagg tctcgggctc
      601 gccgatgccg tagatgcacg agaccgaggc caccaccacc acgtcgcggc gctcgaagag
      661 cgacgcggtg gcggagtgcc gcatgcggtc gatctcgtcg ttcaccagcg cgtccttctc
      721 gatgtaggtg tccgactgcg ggatgtaggc ctcgggctgg tagtagtcgt agtaggagac
      781 gaagtactcg accgcgttgc ccgggaagaa cgccttgaac tcgctgtaga gctgggccgc
      841 gagcgtcttg ttgggcgaga tcaccagcgc ggggcggccg aggcgcgcca tcacgttggc
      901 gagcgtgaag gtcttgccgc tgccggtcac gccgagcagg acggtgtggg ggctgccctc
      961 gccgaccatc gacaccagac gatcgatcgc ctccggctgg tccccggacg gggcgaactc
     1021 ggaggcgagc gtgaacaaca tctggtggtc cgggggaccg ctcaggacgt cgacggcacc
     1081 gccaccaggt acttccgcca cgagtcgagg aacgaggtcg cgtgcggatg ccggagcagg
     1141 tgcaccgaga agaggaactg cgggtgcacc ggctcgcgga tcagccggag ccgcgcctcc
     1201 tccagggtgc gattgccctt gagcaggttg cagcgcaggc aggcggccac cacgttggtc
     1261 caggtggtct ggccgccgcg cgagcgcggc accacgtggt cgaccgtcag cttctccccg
     1321 cggcgcgagc agtactggca ggtgtagccg tcccgtcgca agatgttctt cttgttgaac
     1381 gccacccgct cgaggaacgg cttgcggatg taggccccga gccggatcac ggccgggagg
     1441 tggaagctcc gcgagggcga gcggatcacc cggggcgacg cctcgacgct ctcggccttg
     1501 cccgcgagca gcagcgtgat ggcccgcttg gcgttggtga aatgcagcgg ctcgtaggtg
     1561 tagttcagca ccaggaccgc gatttcatcc atataccccg aaaggataag cgggggcccg
     1621 gaacctgtca actcagcggg gctacaccag gaggcgccag acgaggggac cgaagccggc
     1681 gagcaggacg aggagcatcg tgaacgcctc gctcgaccac gcgaagaggc tgatgagcag
     1741 gcagatcagc gcgaagcacg ccaggtagaa gcggacctcg cgcttgtcca gccagccgcc
     1801 cttgccgacc aggtgcggga tcttgttgat gaggtagatc ggcgagtaca cgaggaagaa
     1861 gatcaccacc agcgccgaga atcccaggac tcccatcatg ccgaacacca cgccccggaa
     1921 gagccagtcc agcagggaga ggttggtgtc gtccttgaga tcgagggcgc gcggcatcgc
     1981 ctcccgatgc tggttcacgt agaggaccgc gagcgagaag agggccagga gcgtctccag
     2041 ccagcgccgg accacgaccc gcgggatgag gctcatcccg gctagaacat cacacgagcc
     2101 gtcggagtgt caacaaaatg ggccgccgcg ccgcgcagtt agagcaggaa gccgcccggg
     2161 aagggatcgc tcgggtcgag cgtcagggtg gaccggccgg tcatccacgc gcggcccgtg
     2221 atctcgggca ccaccgcggc ggtcccggcc acctgggtga gccccgcgat ccgcccggtg
     2281 aagagcgagc cgatgatgga ctcgtgcccg aacccctccc cgaccccgag gcggccgcgc
     2341 gcgtggaggt tggcgagccg cgcggacgtg ccggtgccgc agggcgagcg gtcgaggccg
     2401 gcgggcgcca ccaccaccgc gttgcgcagg tgcgcgtcgg cccgccgcgc cggctcgtgg
     2461 aactggacgt agagcagccc gcgcgcgtcg ggcatcgcgg gatggaccag cggcacgcgc
     2521 gcctcgatgg cgagacggat ccgctcgccc acctccacca gacgcgccgc ctctttcggc
     2581 tcgagggtga ggccgcacgc cgcggcttcc accagcgcgt agaa
//
LOCUS       DATMCV010000732        12037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_573504, whole genome shotgun sequence.
ACCESSION   DATMCV010000732 DATMCV010000000
VERSION     DATMCV010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573504"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VNU03_16750"
     CDS             complement(<1..759)
                     /locus_tag="VNU03_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HWP76175.1"
                     /translation="MPGSNQGPVVACAAYAGGCRVADVAIEDISEVLTQEDRFVWIGL
                     YEPDEPLLKEVQAEFGLHDLAIEDAHLAHQRPKLERYGDSLFIALRTAQPDREQGRIA
                     FGETHLFVGARYVVSVRHGASLSYADVRTRCEASPQLLAKGPGFVLHAIMDFVVDQYF
                     PIVDSLEDDLETLEAHTTMRIYTLKRDLLEIKRGVSPLVDICNRLMRTDLDLIPDDAR
                     PYFRDVYDHAIRLSEKVDGLRELLGTALEANLSLT"
     gene            complement(759..1004)
                     /locus_tag="VNU03_16755"
     CDS             complement(759..1004)
                     /locus_tag="VNU03_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWP76176.1"
                     /translation="MKAYVLIETAAGKTKNVKKALAKIKSVRATVAAMDAVTGPYDFI
                     AIVDGQTLDAIGSLVTDSIGIIDGVTRTTTCVAVTVG"
     gene            complement(1001..1939)
                     /locus_tag="VNU03_16760"
     CDS             complement(1001..1939)
                     /locus_tag="VNU03_16760"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWP76177.1"
                     /translation="MTVHRLEELSTPGLDALDRDRTVVILSVSPLETHGPHLPLGVDA
                     FAARYFSEAIAERIVASRPGWAAVLAPTLHLGSFTFDTVGTVRVRQRVVRDVVVDYGR
                     ALARTGFRHILVSNGHGGPGHLVALEEAAAIVSRRHRVTMASFTGHLAWEFLRGRLLP
                     RIEAGLGRPLSPAERLAFSEDAHGGWWETSLMLALRPDLVDDGYRTLPAARYSLHERL
                     VPNYPLRRGGQGYVGHPAMADPAFARATTDVLLAEAMTLVDALLDGRLRPAERRSPFF
                     AIPLFRTNFRRVAGAGAAAGALLAAWRILRRQRRPR"
     gene            complement(1936..3306)
                     /locus_tag="VNU03_16765"
     CDS             complement(1936..3306)
                     /locus_tag="VNU03_16765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWP76178.1"
                     /translation="MDRFYAYVRMVSRFWLWFFFKDVSVLHAERVPRGGPVLLCINHP
                     NNLIDSLLVGGVLSRKVHYLATAALFRNPLVARFLATCGAIPVYRKQDDPTKMDKNVD
                     AFAACFRALEAGRLVGIYPEGTTHSESRVQRIKTGASRIALDYEAAARGGPLTLIPVG
                     LSFDARKSFRSRVRVSFGEPIPVAPYAAVHREDPARAVEALTTAIQGGMEAEILQVAR
                     PERQDLIRALEQIYRGDLIRELQEERGLGQRQIDPLRLSQAIADAVAHFEERDPERVE
                     RLWAGVQRYRGMLSVYRVRDQAVRARGEHTARRTRLRRSWEAAVGLPFFAYGVAVNGL
                     PYLLPRLIAHRTARKETDYATTRLLASIVAFPLFWALETWIVWRLAGLGWALGFAVSL
                     PISGLLAYRYLGGVGRLQGQLRFGWLSLTHRHFATRLLAERQALVAELEHAKDDYLRA
                     TRGSSF"
     gene            complement(3439..4194)
                     /gene="pncA"
                     /locus_tag="VNU03_16770"
     CDS             complement(3439..4194)
                     /gene="pncA"
                     /locus_tag="VNU03_16770"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HWP76179.1"
                     /translation="MVTDRRGFLKSTMVGALAATAAAGVTRAHAQGALKASPKGTPVK
                     PGPRDVLLVVDVQNDFIPGGALAVKEGDAIVPLVNQLGAGFEHVILTQDWHTPGHVSF
                     ASSHPGKKPFETTTLAYGTQVLWPDHCVQGTPGADLHKDLKIPHAELIIRKGYRKQMD
                     SYSAFFEADGKTTTGLAGYVKDRGFRQVYLVGLATDFCVCWSALDARKVGLGASVIED
                     ACRGIDTNGSLGKAWDQMAKAGVKRIRSADIAV"
     gene            complement(4277..4618)
                     /locus_tag="VNU03_16775"
     CDS             complement(4277..4618)
                     /locus_tag="VNU03_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005420270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HWP76180.1"
                     /translation="MPVHPQWVTTGLEFEGHRIVGHRGVVRGITVRSRNIIVNLGATI
                     QTLFGGNITLYTELCERARQEAFELMLEHASQMGANAVVAMRYDANDVAAGVTEVLAY
                     GTAVVIEPVKP"
     gene            complement(4622..5029)
                     /locus_tag="VNU03_16780"
     CDS             complement(4622..5029)
                     /locus_tag="VNU03_16780"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP76181.1"
                     /translation="MPKLTQAINPPTLVKPAGFSHGWETQGGKTVYIAGQVALDKEGH
                     VVGAGDLVAQFRQVCENLKAVLVSRAGQMNDIVKMTIYVLSKSDYKTRARDIGAVYRD
                     YFGRHYPAMTLVEVKGLYDDACLIEIEAIAVVG"
     gene            5094..6365
                     /locus_tag="VNU03_16785"
     CDS             5094..6365
                     /locus_tag="VNU03_16785"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWP76182.1"
                     /translation="MSRSFEQATLADAVGRLRPGMKVLLPAGCGDPSALLGEILRQAD
                     RLAPLTLMGGLRVDNYPFGAAAYAGKIRFATWHMSPRLAEAAARGDVDFVPARYFDTV
                     RLFAAGGPWAPDAVIVHTAPPDRGGHLSLGVSVSFTLPAARRAPLVIAQVNARMPRTL
                     GNAFLHRSQVDVWTAVDEPLLEYPPTPVGDVERRIAGHVAELIPDGATLQVGVGSIPQ
                     AVMEALSGKKDLGVHSLLVDHMLPLVRSGVITNARKRLHPGRMDVGEIMGTAALFEWS
                     HENPVVNMEPSDVVHDPQVVGTLGDFVSVNSALEVDLLGQVNAESVDGRQVTGIGGQF
                     DFVLGAARAERGRSIIALPATAARGSRSRIVGRLGAGARVTTPRFLADYVVTEHGVAA
                     LRGKSDAGRARALLHIADPAFRELIEREVRP"
     gene            complement(6355..6537)
                     /locus_tag="VNU03_16790"
                     /pseudo
     CDS             complement(6355..6537)
                     /locus_tag="VNU03_16790"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            6654..7637
                     /locus_tag="VNU03_16795"
     CDS             6654..7637
                     /locus_tag="VNU03_16795"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HWP76183.1"
                     /translation="MTCHLLIDTDPGIDDALAILLALASPEAHIEAVTVVAGNVPVDL
                     ATANARRILAVAAPTPMPPVIRGAEAPLKRTLVTAGHVHGQDGLGNLDRFAEPDGRLR
                     YPEPPPIVETRSAAEAILETVDRWGPELTVVALGPLTNLAAAAALDARRLARAGRVVV
                     MGGAVAVSGNVTPAAEFNFFVDPEAAAQVLEAGLAVELIPLDVTRRVVLAQAVLTERL
                     RRCPDDRIARFILDFTLHGFAFGASQEGGGIVLHDPLAMAVALDPSLVTFEPMSVEVE
                     CEGKLTRGLSLADRRALPSHRKRVPSCRVAMDVDADAVLRLFLERLCPASA"
     gene            7619..8545
                     /gene="rbsK"
                     /locus_tag="VNU03_16800"
     CDS             7619..8545
                     /gene="rbsK"
                     /locus_tag="VNU03_16800"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HWP76184.1"
                     /translation="MPRVCVVGSANLDYTVALPRLPRVGETVSSGTLLVNRGGKGANQ
                     AVAARRLGAEVRLIGCVGDDDAGAAMRRALGEQGIGVDGVVTAPGVATGTALIFVDRE
                     GHNQIGVAPGANHRLTVDMARAGEESIAWAQVLVSQLEVPVPVVRWALETANRHGVPT
                     VLNPAPVQELSDDLLGLVTYLTPNAGEVAALTGIEVTDLDSGRRAAARLCERGVGTVI
                     ITLGEEGALVCDGTTAVHFEAFPVEAIDTTGAGDAFNGALAVGLSAGGSLEQVIPLAS
                     AAAALTCTRRGAQDALPARADVEHFLQSLRDR"
     gene            complement(8567..9703)
                     /locus_tag="VNU03_16805"
     CDS             complement(8567..9703)
                     /locus_tag="VNU03_16805"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWP76185.1"
                     /translation="MRVLPGRAALVFLSLGLALTGCALITPRPAPDPARLRIADLLLI
                     GFTGTQVAGNEEVRRLVCDVKVGGLLLFERYTSGQPRNMVSPEQISTLTRDLQALAAR
                     CVGRPLFIAVDAEGGRVMRLSPRLGYPPTRPAQELGERDDPAFTQAEARRMGLMLREA
                     GINWDLAPVVDVAVNPANPAVVALGRTYSADPARVSVHARAFVEGMHEAGVLTALKHF
                     PGHGSSRADSHHGFTDVTDTANLSVELQPYRALIAEGMADSVMTAHVFNRGLDPWDPA
                     TLSRFTVRRVLRGWLHYDGVAVSDDLLMGAIAQHYGLEDAAVRALDAGVDVLLISQNT
                     GRIDDRAAERVVAAITRAIAEGRLAESAVTTAVRRVDLLRARLK"
     gene            complement(9748..10563)
                     /locus_tag="VNU03_16810"
     CDS             complement(9748..10563)
                     /locus_tag="VNU03_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HWP76186.1"
                     /translation="MSLAAIIGLPAHVTRAIIGMVHLRALPGSPRWDGDMDGVVRAAL
                     DDARALADGGVDALVVENHGDVPFTAGRVDAASVAGMTVAVAEIRRQVTRPIGINVLK
                     NDVRSALAVAAATGARFVRVNVHVGAVAADQGIIQSEAHDSLRYRRLLGVQIAILADV
                     QAKHGMPLAPVPIEHEARDCYARGLADALVVSGVATGEPTPMSDLKRVRGAVPEAPLL
                     VGSGASPETVAELLSVADGVIVGTSLKRDGRLANPVDVERVRRLVAAARGARG"
     gene            10588..11889
                     /locus_tag="VNU03_16815"
     CDS             10588..11889
                     /locus_tag="VNU03_16815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP76187.1"
                     /translation="MLRTPADAPARIFYDYWVVLALSLIVLLSMGIRFAIGPFLKPVS
                     AELGIDRGTFSLVISLSLFLYGAFMPLVGRVVDRFGSRVVCSVGAVVMAASLVATARM
                     TTLWEFYLYYAVVGSLGLAATGHVMGSVALARWFVRRRGLAMSTLGAAGMAGMALVVP
                     VAMWCILRYGWRATFVILGVGSLAIMLPLTLWVLRDGPESMGLEPDGDAAPAPAPATR
                     TIERTAIGDALRVPSFWLLSVGLFNCGFSMSLLSAHGVPMLTDHGFHPMTASSAIGFL
                     GMTAIGGGLLLGLISDRWGRTPVLASVYVLRLIAFGMLFFVRDPLLLLVVAAVGGVGM
                     SGSLAMTSALTGDIFGRYSVGSIFGLIFLSHQTGAALGSWLGGTLFDLTGGYGAAFSV
                     AGALLLIAAGLSITINEAARPAGRAMTLPGRREPHPVAGGR"
BASE COUNT         1595 a         4211 c         4493 g         1738 t
ORIGIN      
        1 ggtgagcgag aggttggcct cgagcgcggt gccgagcagc tcgcgcagcc cgtccacctt
       61 ctcgctcagg cggatcgcgt ggtcgtagac gtcgcggaag taggggcgcg cgtcgtcggg
      121 gatcaggtcg aggtcggtgc gcatgagccg gttgcagatg tcgaccaggg gcgagacccc
      181 gcgcttgatc tcgagcaggt cgcgcttgag ggtgtagatg cgcatggtgg tatgggcctc
      241 gagcgtctcc agatcgtcct cgagcgagtc cacgatcggg aagtactggt ccaccacgaa
      301 gtccatgatc gcgtgcagca cgaagccggg ccccttggcc aggagctgcg ggctcgcctc
      361 gcagcgggta cggacgtcgg cgtaggagag cgaggccccg tgccggaccg agaccacgta
      421 gcgggccccc acgaagagat gggtctcccc gaaggcgatc cggccctgct cgcggtcggg
      481 ctgggcggtg cggagcgcga tgaagagcga gtcgccgtag cgctcgagct tgggccgctg
      541 gtgggccagg tgggcgtcct cgatggcgag gtcgtgcagg ccgaactcgg cctgcacctc
      601 cttgagcagg ggctcgtcgg gctcgtagag gccgatccag acgaagcggt cctcctgggt
      661 caggacctcg ctgatgtcct cgatcgccac gtccgccacc cggcagccgc ccgcgtaggc
      721 ggcgcaggcg accaccggtc cctggttcga tcccggcatc agccgaccgt gaccgccacg
      781 caggtggtgg tgcgcgtcac cccgtcgatg atcccgatgg agtcggtgac gaggctgccg
      841 atggcgtcga gggtctggcc gtccacgatg gcgatgaagt cgtagggccc ggtgacggcg
      901 tccatggccg ccaccgtcgc gcggacgctc ttgatcttgg cgagcgcctt cttgacgttc
      961 ttggtcttgc cggccgcggt ctcgatcagc acgtaggctt tcatcgtggt ctcctttgtc
     1021 tgcgcaggat ccgccaggcg gccaggaggg cgcccgcggc cgcgccggct cccgccaccc
     1081 ggcggaagtt ggtgcggaag agcgggatgg cgaagaaggg cgaccgacgc tcggcggggc
     1141 ggagccgtcc gtcgagcagg gcgtcgacca gggtcatggc ctccgcgagc agcacgtcgg
     1201 tggtcgcccg cgcgaaggcg ggatcggcca tggccgggtg ccccacgtag ccctgcccgc
     1261 cgcggcgcag gggatagttg ggcacgaggc gctcgtggag ggaatagcgg gcggccggca
     1321 gcgtgcggta gccgtcgtcg accaggtcgg gccgcagcgc cagcatcagc gacgtctccc
     1381 accagccgcc gtgggcgtcc tcggagaacg cgaggcgctc ggccggcgag agcgggcgcc
     1441 ccaggccggc ctcgatccgg ggcagcaggc gaccgcgcag gaactcccac gcgaggtggc
     1501 cggtgaacga ggccatggtg acccggtgcc ggcgcgagac gatggcggcc gcctcctcga
     1561 gggccaccag gtgaccgggg ccgccgtggc cgttcgagac caggatgtgg cggaagccgg
     1621 tgcgggcgag cgcgcgcccg tagtccacga ccacgtcgcg gactaccctc tgccgtaccc
     1681 ggaccgtgcc gacggtgtcg aacgtgaacg agcccaggtg gagcgtgggc gccagcaccg
     1741 ccgcccagcc cgggcggctc gcgacgatgc gctcggcgat cgcttccgag aaatagcgcg
     1801 cggcgaacgc gtcgacgccg agcggcaggt gcggcccgtg ggtctcgagc gggctgaccg
     1861 agaggatcac cacggtgcga tcgcggtcga gcgcgtcgag ccccggggtg gagagctcct
     1921 ccaggcgatg gacggtcaaa agctgctgcc ccgcgtcgcc cggaggtagt cgtccttggc
     1981 gtgctcgagc tcggccacca gcgcctgccg ctcggccagc aggcgggtcg cgaagtggcg
     2041 gtgggtcagc gagagccagc cgaaccggag ctggccctgc agccggccca cgccgcccag
     2101 gtagcgatac gcgagcaggc cgctgatcgg cagcgagacc gcgaagccga gcgcccagcc
     2161 gagccccgcg agacgccaga cgatccaggt ctcgagcgcc cagaagagcg ggaaggccac
     2221 gatgctggcg aggaggcggg tggtcgcgta gtccgtctcc ttgcgcgcgg tgcggtgggc
     2281 gatcaggcgc ggcagcaggt agggcaggcc gttgacggcg acgccgtagg cgaagaacgg
     2341 gagcccgacc gcggcctccc aggagcggcg caggcgggtg cggcgcgcgg tgtgctcgcc
     2401 gcgggcgcgt accgcctgat cgcgcacccg gtacaccgag agcatgccgc gatagcgctg
     2461 cacgcccgcc cagagccgct ccacccgctc cgggtcccgc tcctcgaagt gggcgaccgc
     2521 gtccgcgatg gcctgcgaga ggcgcagcgg gtcgatctgc cgctggccca ggccacgctc
     2581 ctcctgcagc tcccggatca ggtcgccgcg gtagatctgc tcgagggcgc ggatgagatc
     2641 ctggcgctcg ggacgggcca cctggaggat ctccgcctcc atgcccccct ggatcgcggt
     2701 ggtcagggcc tccaccgcgc gggccgggtc ctcccgatgc accgccgcgt acggggccac
     2761 cgggatcggc tcgccgaagg agacgcgcac gcggctccgg aacgacttgc gggcgtcgaa
     2821 gctcaggccg accgggatca aggtgagggg gccgccccgg gcggcggcct cgtagtccag
     2881 cgcgatgcgg gaggcgccgg tcttgatccg ctgcacgcgg ctctcggagt gggtcgtgcc
     2941 ttccgggtag atgccgacga gccgcccggc ttcgagggct cggaagcagg ccgcgaaggc
     3001 gtccacgttc ttgtccatct tggtgggatc gtcctgcttg cggtagaccg ggatggcgcc
     3061 gcaggtggcc aggaatcgag ccacgagcgg attgcggaag agcgcggccg tggccaggta
     3121 gtggaccttg cgggagagga ccccgcccac gaggagcgag tcgatcaggt tgttggggtg
     3181 gttgatgcac aggaggacgg gcccgccgcg cggcacgcgc tcggcatgga gcaccgagac
     3241 gtccttgaag aagaaccaga gccagaaccg gctaaccatc cgcacgtacg catagaatcg
     3301 atccatgcgc tccggtggct gggtcgcggc cgcctggacg ctcatcggga gaggggcgcg
     3361 cgcggcggat cggcccaggc caggacctcg gccagcagca gcgcccggac cccggcccgg
     3421 cggcccgtca ccgcggtgct agaccgcgat gtcggcggag cggatgcgct tcaccccggc
     3481 cttggccatc tggtcccacg ccttgccgag cgagccgttg gtgtcgatgc cccggcaggc
     3541 gtcctcgatc accgaggcgc ccaggcccac cttgcgcgcg tcgagcgccg accagcagac
     3601 gcagaagtcg gtggcgagcc cgaccaggta gacctggcgg aagccgcggt ccttcacgta
     3661 gccggcgagc ccggtggtgg tcttgccgtc ggcctcgaag aaggcggagt aggagtccat
     3721 ctgcttgcga tagcccttgc ggatgatcag ctcggcgtgc gggatcttga ggtccttgtg
     3781 gaggtcggcc cccggcgtcc cctgaacgca gtgatccggc cagagcacct gggtgccgta
     3841 ggcgagcgtg gtggtctcga acggcttctt gcccgggtgc gaggaggcga aggagacgtg
     3901 gcccggggtg tgccagtcct gggtgaggat gacgtgctcg aagccggccc cgagctggtt
     3961 cacgagcggc acgatcgcgt cgccttcctt gaccgcgagg gccccgccgg ggatgaagtc
     4021 gttctgcacg tccacgacca ggagcacgtc gcggggtccg ggcttgaccg gggtgccctt
     4081 gggggaggcc ttcagcgcgc cctgcgcgtg cgcccgcgtc accccggcgg ccgccgtcgc
     4141 ggccagcgcg cccaccatcg tgctcttgag gaacccccgg cgatccgtga ccatgacgtt
     4201 ctcctccttc ggcccgcgag tggacggctc gcccgagagt atagtctcgc gcgagcggtg
     4261 gaggccaggg gctcggctag ggcttcaccg gctcgatcac caccgcggtg ccgtaggcga
     4321 ggacctcggt cacgccggcc gccacgtcgt tggcgtcgta gcgcatcgcc accaccgcgt
     4381 tggcgcccat ctgggaggcg tgctccagca tcagctcgaa cgcctcctgg cgcgcgcgct
     4441 cgcagagctc ggtgtagagc gtgatgttgc ccccgaagag ggtctggatc gtcgccccca
     4501 ggttgacgat gatgttccgg gagcgcaccg tgatgccgcg caccacgccc cgatgtccca
     4561 cgatgcggtg cccctcgaac tcgagcccgg tcgtcaccca ctgcgggtgc accggcatcg
     4621 gtcagcccac caccgcgatc gcttcgatct cgatcaggca cgcgtcgtcg tagaggccct
     4681 tgacctccac cagggtcatg gccgggtagt ggcgcccgaa gtagtcgcgg tagaccgcgc
     4741 cgatgtcccg cgcgcgggtc ttgtagtcgc tcttggacag cacgtagatc gtcatcttga
     4801 cgatgtcgtt catctgcccc gcgcgggaca ccagcaccgc cttcaggttc tcgcacacct
     4861 ggcggaactg ggcgaccaga tcgcccgcgc ccacgacgtg gccctccttg tcgagcgcga
     4921 cctggcccgc gatgtagacc gtcttgccgc cctgggtctc ccagccgtgg gaaaagccgg
     4981 ccggcttgac gagggtcggg gggttgatgg cttgcgtgag cttgggcatg gattcctccc
     5041 gggacagacg cgttgagtgg ccgatccgcg cgcgattata ctacccgcgc tccatgagcc
     5101 gttccttcga gcaggccacg ctggccgacg cggtcggccg actccgaccg gggatgaagg
     5161 tgttgctccc ggccggctgc ggcgatccct ccgcgctgct cggggagatc ctgcggcagg
     5221 ccgatcggct cgcgccgctg accctgatgg gcgggctgcg cgtcgacaat tatccgttcg
     5281 gcgcggcggc ctatgcgggc aagatccgct tcgcgacctg gcacatgtcg ccgcggctcg
     5341 cggaggcggc ggcccggggc gacgtggact tcgtgcccgc gcgctacttc gacacggtgc
     5401 gcctcttcgc ggcgggcggg ccgtgggcgc cggacgcggt gatcgtccac accgcgccgc
     5461 ccgaccgggg cgggcacctc tcgctcggag tctcggtgag cttcacgctg cccgccgcgc
     5521 gccgcgcccc gctggtcatc gcgcaggtga acgcgcgcat gcctcgcacc ctcggcaacg
     5581 cgttcctgca ccgctcgcag gtcgacgtct ggaccgcggt cgacgagccg ctgctcgagt
     5641 acccgccgac cccggtcggc gacgtggagc ggcgcatcgc ggggcacgtg gccgagctca
     5701 tcccggacgg ggcgacgctc caggtcgggg tcggctccat cccccaggcg gtcatggagg
     5761 cgctctcggg caagaaggac ctgggggtgc actcgctcct ggtcgaccac atgctgcccc
     5821 tggtgcggag cggggtcatc accaacgcgc gcaagcgcct ccatcccggc cgcatggacg
     5881 tgggggagat catgggcacc gcggcgctct tcgagtggag ccacgagaac ccggtggtga
     5941 acatggagcc gtccgacgtg gtccacgacc cgcaggtggt gggcacgctc ggcgacttcg
     6001 tctcggtcaa ctccgccctg gaggtggacc tgctcgggca ggtcaacgcg gagagcgtgg
     6061 acgggcggca ggtgaccggg atcggcggcc agttcgactt cgtgctgggc gcggcgcgcg
     6121 cggagcgggg ccgctcgatc atcgcgctgc ccgcgaccgc ggcgcggggc agccggtcgc
     6181 ggatcgtggg gcggctcggg gcgggagcgc gcgtgacgac gccacgcttc ctcgccgact
     6241 acgtggtcac cgagcacggg gtggcggcgc tgaggggaaa aagcgacgcc gggcgggcga
     6301 gggcgctgct ccacatcgcc gacccggcgt tccgggagct gatcgagcgg gaggtcaggc
     6361 cttgagcttg tcgatctcgg cgaagacctc ttgatcctcc gggaagcggt acgtcacctg
     6421 gttgtcgtag atcatcttgt cgtcggcctc gacggtgaag atgcccccga cgccctcccg
     6481 cagctcggag tcgatgccgt acttactctt gatggcggcc tgcaaactgg aggcctgggg
     6541 tagatagttt cactcgccgc agtacgtgat ccggatgtgc atccgagtcc tcctttgcga
     6601 ggtactggcg ccaggctgcc gcgtatgata cggaccctcg gagagggtgt caaatgacct
     6661 gccacctgct gatcgacacc gaccccggca tcgacgacgc gctcgcgatc ctgctcgcgc
     6721 tcgcctcgcc ggaggctcac atcgaggcgg tcaccgtggt cgccggcaac gtgccggtgg
     6781 atctggccac cgccaatgcc cggcgcatcc tcgcggtggc cgccccgacc ccgatgccgc
     6841 cggtgatccg gggcgcggag gcgccgctca agcggaccct ggtcaccgcg gggcacgtcc
     6901 acggccagga cgggctcggc aacctggacc gcttcgccga gcccgacggc cggctccgct
     6961 atccggaacc gcctcccatc gttgagacgc ggtcggccgc ggaagcgatt ctggagacgg
     7021 tcgaccggtg gggtccagag ctgacggtgg tggccctcgg gccgctgacg aacctggccg
     7081 ccgcggcggc gctggacgcg cggcggctcg cgcgggccgg gcgcgtggtg gtgatgggcg
     7141 gggcggtcgc ggtatcgggc aacgtgaccc cggcggccga gttcaacttc ttcgtggatc
     7201 cggaggccgc cgcccaggtg ctggaggccg ggctcgccgt cgagctcatc ccgctcgacg
     7261 tgacccgccg ggtggtcctg gcgcaggcgg tcctcaccga gcggctgcgg cgctgcccgg
     7321 acgaccggat cgcccgcttc atcctcgact tcaccctgca cggcttcgcc ttcggcgcct
     7381 cgcaggaggg gggcggcatc gtgctgcacg atccgctcgc gatggcggtg gcgctcgacc
     7441 cgtcgctcgt caccttcgag ccgatgagcg tggaggtcga gtgcgagggc aagctgaccc
     7501 ggggcctctc cctggccgat cgccgtgccc tcccctcgca ccgtaagcgg gtgccgagct
     7561 gccgggtcgc gatggacgtc gacgcggacg cggtgctgcg gctcttcctg gagcgcctgt
     7621 gccccgcgtc tgcgtagtcg gctccgccaa cctcgactac accgtcgcgc tcccgcgcct
     7681 gccgcgcgtc ggcgagaccg tctcctcggg cacgctgctg gtcaaccgcg gcggcaaggg
     7741 cgcgaaccag gcggtggccg cgcggcgcct cggggcggag gtgcggctca tcggctgcgt
     7801 gggcgacgac gacgcgggag ccgcgatgcg ccgcgccctc ggcgagcagg ggatcggcgt
     7861 cgacggcgtg gtgaccgcgc ccggagtggc caccgggacc gcgctcatct tcgtggaccg
     7921 ggaggggcac aaccagatcg gggtggcgcc gggggccaat caccggctca ccgtggacat
     7981 ggcgcgggcg ggggaggagt cgatcgcgtg ggcgcaggtc ctggtcagcc agctcgaggt
     8041 gccggtcccg gtcgtccgct gggctctcga gaccgcgaac cgccacgggg tgccgaccgt
     8101 gctcaatccg gcgccggtcc aggagctgtc cgacgatctg ctggggctgg tgacctacct
     8161 caccccgaac gcgggggagg tggccgcgct caccggcatc gaggtgaccg acctcgactc
     8221 ggggcggcgg gccgccgcgc ggctctgcga gcggggcgtg ggcaccgtga tcatcacgct
     8281 gggtgaggaa ggcgcgctcg tctgcgacgg cacgaccgcg gtgcacttcg aggccttccc
     8341 ggtcgaggcg atcgacacca ccggggccgg cgacgccttc aacggcgcgc tcgcggtggg
     8401 cctctccgcc ggcggcagcc tcgagcaggt gatcccgctc gccagcgccg ccgccgcgct
     8461 cacctgcacg cgccgcggcg cccaggacgc gctccccgcc cgcgccgacg tcgagcactt
     8521 cctccagtcc ctccgcgacc gctgaagggg tcataagacc tgccccctac ttcagccgcg
     8581 cgcggaggag gtcgacgcgg cggaccgcgg tggtgacggc ggactcggcg aggcggccct
     8641 cggcgatggc gcgggtgatg gcggcgacca cgcgctcggc cgcgcggtcg tcgatgcggc
     8701 cggtgttctg ggagatcagg agcacgtcga cgcccgcgtc cagggcccgc accgccgcgt
     8761 cctcgagccc gtagtgctgg gcgatcgcgc ccatcagcag gtcgtcggac accgccaccc
     8821 cgtcgtagtg gagccagccc cgcagcacgc ggcgcaccgt gaagcgcgag agcgtggcgg
     8881 ggtcccacgg gtcgaggccg cggttgaaca cgtgcgcggt catcacgctg tcggccatcc
     8941 cctccgcgat cagggcgcgg tacggctgga gctcgaccga caggttcgcg gtgtcggtca
     9001 cgtcggtgaa gccgtggtgc gagtccgccc ggctcgagcc gtggccgggg aagtgcttga
     9061 gcgcggtgag tacccccgcc tcgtgcatgc cctcgacgaa cgcccgcgcg tgcacgctca
     9121 cccgcgcggg atcggccgag tacgtccgcc cgagcgcgac gaccgcggga ttcgccggat
     9181 tgaccgcgac gtccaccacc ggagcgaggt cccagttgat gccggcctcg cgcagcatga
     9241 gccccatgcg ccgcgcctcc gcctgggtga acgcggggtc gtcgcgctcg cccagctcct
     9301 gcgcgggccg ggtgggcgga tagccgaggc gcggggagag ccgcatcacc cggccgccct
     9361 cggcatccac cgcgatgaag agcggccggc cgacgcagcg cgcggcgagg gcctgcagat
     9421 cgcgtgtcaa agtcgagatc tgctccgggc tcaccatgtt gcgcggctgt cccgacgtgt
     9481 agcgctcgaa gaggaggagg ccgccgacct tcacgtcgca cacgaggcgg cgcacctcct
     9541 cgttgcccgc gacctgggtg ccggtgaagc cgatgaggag gagatcggcg atgcggaggc
     9601 gcgcgggatc gggagcgggg cggggggtga tgagggcgca gccggtgaga gctagcccga
     9661 gggagaggaa gaccagcgcg gcgcggccgg ggagcaccct caccccggcc ctctccctct
     9721 gagagggaga gggggaaggg cgggcgatca cccgcgggcg ccccgggccg cggcgacgag
     9781 gcggcggacg cgctccacgt cgaccgggtt ggcgaggcgg ccgtcgcgct tgagcgaggt
     9841 gccgacgatc acgccgtcgg ccaccgacag cagctcggcc accgtctcgg gcgacgcccc
     9901 gctcccgacg agcagggggg cctccgggac cgccccgcgc acccgcttga gatcgctcat
     9961 cggggtgggc tcgccggtgg ccacgcccga caccacgagc gcgtcggcca gcccgcgcgc
    10021 gtagcagtcg cgcgcctcgt gctcgatcgg caccggggcc agcggcatgc cgtgcttggc
    10081 ctgcacgtcg gcgaggatcg cgatctggac cccgaggagc cggcggtagc gcagggagtc
    10141 gtgcgcctcg gactggatga tgccctggtc cgccgcgacc gcgcccacgt gcacgttgac
    10201 gcgcacgaag cgcgcgccgg tggcggcggc gaccgccagc gcggagcgca cgtcgttctt
    10261 cagcacgttg atcccgatcg gccgcgtcac ctgccggcgg atctccgcca ccgcgaccgt
    10321 catcccggcc accgaggccg cgtcgacgcg gcccgcggtg aaggggacat caccgtggtt
    10381 ctccaccacc agcgcgtcga cgccgccgtc ggcgagggcc cgcgcgtcgt cgagcgcggc
    10441 ccgcaccacc ccgtccatgt cgccgtccca gcgcgggctg ccgggcagcg cgcgcaggtg
    10501 caccatgccg atgatggcgc gcgtgacgtg cgcgggcagg ccgatgatcg cggcgagact
    10561 catctggcgg gatgatatca tttcgtcatg ctgaggacgc ccgccgacgc gcccgcccgc
    10621 atcttctacg actactgggt ggtcctggcg ctctcgctca tcgtgctcct ctccatgggc
    10681 atccgcttcg cgatcggccc gttcctcaag ccggtgtcgg cggagctcgg catcgatcgt
    10741 ggcaccttct ccctcgtgat ctcgctctcg ctcttcctgt acggcgcgtt catgccgctg
    10801 gtcgggcgcg tggtggatcg cttcggctcg cgggtggtct gctcggtggg cgcggtggtg
    10861 atggcggcct cgctggtggc caccgcccgc atgaccacgc tgtgggagtt ctacctgtac
    10921 tacgcggtgg tgggctcgct cgggctggcc gccaccgggc acgtgatggg ctcggtcgcc
    10981 ctcgcccgct ggttcgtgcg ccgccgcggg ctcgcgatga gcacgctggg cgcggccggc
    11041 atggcgggca tggccctggt ggtgccggtg gcgatgtggt gcatcctgcg ctacggctgg
    11101 cgcgcgacct tcgtcatcct gggcgtgggc tcgctcgcga tcatgctgcc cctcaccctg
    11161 tgggtgctcc gcgacgggcc ggagagcatg ggcctcgagc cagacggcga cgcggccccc
    11221 gcgccggctc cggccacccg cacgatcgag cggaccgcga tcggcgacgc gctgcgggtg
    11281 ccgtccttct ggctcctgag cgtggggctc ttcaactgcg gcttctcgat gagcctgctc
    11341 tccgcgcacg gggtgccgat gctcaccgac cacggcttcc acccgatgac cgcctcctcc
    11401 gcgatcggct tcctcggcat gaccgcgatc gggggaggcc tcctgctcgg cctgatctcg
    11461 gaccgctggg gccgtacccc ggtgctcgcc tccgtgtacg tgctgcgcct gatcgccttc
    11521 ggcatgctct tcttcgtgcg cgacccgctg ctcctgctgg tggtggccgc cgtcggcgga
    11581 gtcgggatgt cgggcagcct ggccatgacc tccgcgctga ccggggacat cttcggccgg
    11641 tactcggtgg gctccatctt cgggctgatc ttcctctccc accagacggg cgcggcgctc
    11701 ggctcgtggc tgggcggcac gctcttcgac ctgacgggcg gctacggggc cgccttctcg
    11761 gtggcgggcg ccctgctgct gatcgcggcc gggctcagca tcacgatcaa cgaggcggcg
    11821 cgcccggcgg gccgcgcgat gacgctgccg gggcgacgcg agccgcaccc ggtggccgga
    11881 ggacgctgat gggctacagc acgatcctgg tggaccggac cgacggggtg gccaccatca
    11941 cgctcaaccg gccggaggcg cgcaacgcgc tcgacctggt gatgcggcgc gagctgctca
    12001 ccgcgctcga cgacctggag gcggacgcgg ccacccg
//
LOCUS       DATMCV010000733         4753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_442635, whole genome shotgun sequence.
ACCESSION   DATMCV010000733 DATMCV010000000
VERSION     DATMCV010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442635"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VNU03_16820"
     CDS             <1..349
                     /locus_tag="VNU03_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76188.1"
                     /translation="CARTPLSTPAAPDVKALTGSWQGWLVTERDFTLVRFDIHEDATF
                     EVTGPRIRATGTLTLVGGLVRFSGTGLWQGSLTPTPPGGGGALRLERDDRLYRGTLRL
                     ARDSALPTSGNLR"
     gene            350..679
                     /locus_tag="VNU03_16825"
     CDS             350..679
                     /locus_tag="VNU03_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76189.1"
                     /translation="MYPTIMRVRSRLSRSIGAVTSTLILFVFALAFQLDAMRPPHAHG
                     DETTGIYNEEHILAELGSLHRIVSLPEGAPAIVLALRTTAAVVSARVHPHSPDASPAG
                     PRAPPAA"
     gene            767..2188
                     /locus_tag="VNU03_16830"
     CDS             767..2188
                     /locus_tag="VNU03_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76190.1"
                     /translation="MRSLISICLLIGGALLPLPAEAQEVESLRKELEQMRKQFDTMKD
                     GYEKAINQLGERIQQLENRPQPAAAPPPAAPPPAAVAQAPAPPASAVTINPMDLARPR
                     EPFSLYGQRGPGQLLFDIGVAGDFIGNLTQSNVDKANAGSFPGRENRFFPREIELALF
                     GQIDPYARAEVRFEAGEEDRGEIALHLAEANLTLMALPFGTQLKMGQMRNRFGLLNPV
                     HEHDRPFIDAPNVLARFLGEEGLVERGAELTWVAPLPFFLEALVGAFNGDNDTAFGRG
                     KLNAPLVTGRLRTFFELGDESAIQLGASVANGETSERHRNTLLGFDAKYKYRPEGWQH
                     PLLTLLGEMIYQNRRVNVLGEDTNGDGIQDTPDQTRTRNRWGWYVGGEVQPFRTGQLA
                     RWAAGFRYDWTQYPVMRGREWAVGPYLAFMPSEFLRFRLGYKYTERADRDGFTDNGGS
                     ARKVDEILFQATFILGAHPAHPF"
     gene            2214..3155
                     /locus_tag="VNU03_16835"
     CDS             2214..3155
                     /locus_tag="VNU03_16835"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWP76191.1"
                     /translation="MRPLARMLSPLCLLLVFGIGLGIGLEAGLAEAASKIPVVATTTD
                     LKALVEAVGGDLVEVDALARGTQNPHDLEVRPSLMVKVRRADLLVMNGLELDQWAEVV
                     VQGAGNPRVGLGAPGRVDASAGLLVLEVPQGKVDRSMGDVHPVGNPHYSLDPGMAAGV
                     TANIVEGLARVAPQSRPTFDRNRQAFLGRVDQALTRWTTTLAPFKGTKVVVDHNMWIY
                     FLTRFGLVEAGSIEERPGIPPTPSHLTKLIALMKDQKVRVILSVPWGDQKIAERVAQE
                     TGAKVVPLAPAVGSAKGTDGYLETIDYNVRSVAQALK"
     gene            3187..4053
                     /locus_tag="VNU03_16840"
     CDS             3187..4053
                     /locus_tag="VNU03_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWP76192.1"
                     /translation="MSGDLLALMWFPFLMCLVLTGIHAYLGFHVIAREVVFVDIALAQ
                     IAALGATAAFLLGYELETWESYAAGLGFTVLGALVLALTRSRTRRVSHEAVIGVVYAV
                     SSAGAVLLADRSPHGAEHLRGMLVGSILSVRGDEVLKVAILYALVGLFHWLCRRPFFL
                     ISTDPDRAYREGWRVRAWDFAFYASFGVVVTSSVRIAGVLLVFSYLIVPALAGILLAS
                     RIGARLAVGWAFGAGVSIMAMVASAVIDLPTGATVVCAFGLSLFVLGLLLRLRPRPLG
                     AAALADPGGGHA"
     gene            4050..>4753
                     /locus_tag="VNU03_16845"
     CDS             4050..>4753
                     /locus_tag="VNU03_16845"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HWP76193.1"
                     /translation="MTGRLRGIALAAILALAGCQESPAPAKPQVIATIYPLWELTRQV
                     AGDRAEVTSLVPPGVEPHDWEPSPRDVSLVQRASVIVHTGTGLDAWAVRLLAALPART
                     TVVNAARGLSLLPQGPSVDPHVWLDPTLASVQAQTIADGLAQADSAGRPVYLENAKAL
                     AARLGALDQAFAAGLRDCARREVVTTHAAFAYLARHYQLTQVPLMGSAPEAEPSPADL
                     AAIVQTARRLHVTHVF"
BASE COUNT          636 a         1816 c         1586 g          715 t
ORIGIN      
        1 ttgcgcccgc acgcctctct cgacccccgc ggctccggac gtcaaagcgc tgaccggatc
       61 atggcagggc tggctcgtga ccgagcggga cttcacgctg gtacgcttcg atatccacga
      121 ggacgcgacg ttcgaggtca ccggccctcg gatccgggcg accggaacgc tgacgctggt
      181 cgggggtctg gtgcgcttca gcgggaccgg cctgtggcag ggatccctga cccccacgcc
      241 gccgggaggc gggggtgccc tcaggctcga gcgggacgac cgtctctacc gaggaacact
      301 ccgtctcgcc cgagattcgg ccttgccaac gtccggaaat ctgcgataag tgtacccgac
      361 catcatgcgg gtccggtctc gcctctcgcg ttccatcggc gcggtcacct cgacgctgat
      421 cctcttcgtc ttcgcgctcg cgttccagct cgatgcgatg cgcccgccgc acgcccacgg
      481 ggacgagacc accgggatct acaacgagga gcacatcctc gccgagctgg gatcgctgca
      541 ccgtatcgta tcgctccccg aaggggcacc ggcgatcgtc ctggccctcc gcacgactgc
      601 ggcggtggtc tccgcccgcg tgcacccgca ctcgccggac gccagtcccg ccgggccccg
      661 agcgccgccc gccgcctagc ctcgcgccgt cctcgcatcg tcgccgatcc acgggatctc
      721 cagatcccgc cggaccgggt ccaatcctct cgagacggag gcgtccatgc gctcactcat
      781 cagtatctgt ctgctcatcg ggggcgcgct gctccctctt ccggcggagg cccaggaggt
      841 cgaatccctg cggaaggagc tggagcagat gcgcaagcag ttcgacacca tgaaggacgg
      901 ctacgagaag gcgatcaacc agctgggtga gcgcatccag cagctggaga atcgcccgca
      961 gcccgccgcg gcgccaccgc cggccgcgcc gccgcccgcg gcggtcgccc aggcccccgc
     1021 cccgccggct tccgcggtca cgatcaaccc gatggacctg gcgcggcccc gtgagccctt
     1081 ctccctctac ggccagcgcg ggcccgggca gctcctgttc gacatcggcg tggccggcga
     1141 cttcatcggc aacctcaccc agagcaatgt cgacaaggcc aacgcgggct cgttcccggg
     1201 gcgcgagaac cgcttcttcc cgcgcgagat cgagctggcg ctcttcggcc agatcgatcc
     1261 ctacgctcgc gcggaggtcc gcttcgaggc cggcgaggag gatcggggcg agatcgcgct
     1321 ccacctggcc gaggccaacc tgaccctgat ggcgctgccc ttcggcaccc agctcaagat
     1381 gggccagatg cgcaaccgct tcgggctgct caatccggtg cacgagcacg accggccgtt
     1441 catcgacgcg cccaacgtgc tggctcgctt cctcggcgag gaggggctcg tcgagcgcgg
     1501 cgccgagctc acctgggtgg cgccgctgcc cttcttcctc gaggcgctcg tcggcgcgtt
     1561 caacggcgac aacgacaccg ccttcgggcg cggcaagctc aacgcgccgc tcgtgaccgg
     1621 ccgcctgcgc accttcttcg agctgggcga cgagagcgcc atccagctcg gggcctcggt
     1681 ggccaacggc gagacgtccg agcgccatcg caacaccctg ctcggcttcg acgccaagta
     1741 caagtaccgg cccgagggct ggcagcatcc gctgctcacc ctgctggggg agatgatcta
     1801 ccagaaccgg cgcgtcaacg tgctgggcga ggacaccaac ggcgacggca tccaggacac
     1861 gccggaccag acccgcaccc gcaaccgctg gggctggtac gtgggcggcg aggtgcagcc
     1921 cttccgcacc ggccagctcg cccgctgggc cgcgggcttc cgctacgact ggacccagta
     1981 cccggtcatg cgtggccgcg agtgggcggt gggcccgtac ctcgcgttca tgccgtccga
     2041 attcctgcgc ttccgcctcg gctacaagta caccgagcgg gccgaccggg acggcttcac
     2101 cgacaacggc gggagcgccc ggaaggtcga cgagatcctc ttccaggcca cgttcatcct
     2161 gggcgcccat ccggcgcacc cgttctagac tcgacgagga gacgatcatg cccatgcgcc
     2221 cgctcgctcg catgctgagc ccgctctgcc tcctgctcgt cttcgggatc ggcctcggga
     2281 ttggcctcga ggccgggctc gcggaggccg cgtccaagat tcccgtcgtg gcgaccacga
     2341 ccgacctcaa ggccctggtc gaggcggtgg gcggcgacct ggtcgaggtc gacgcgctcg
     2401 cccgcggcac ccagaacccg cacgacctcg aggtccggcc gagcctcatg gtcaaggtcc
     2461 gccgggccga cctgctggtc atgaacggcc tcgagctcga ccagtgggcc gaggtggtcg
     2521 tgcagggggc cggcaaccca cgggtggggc tcggcgcgcc ggggcgggtc gacgcctcgg
     2581 ccggcctcct cgtgctcgag gtgccccagg gcaaggtcga ccgctcgatg ggcgacgtgc
     2641 acccggtggg caatccccac tactccctcg acccgggcat ggcggccgga gtcaccgcca
     2701 acatcgtcga agggctcgcg cgggtcgcgc cccagtcccg tcccacgttc gatcgcaacc
     2761 ggcaggcctt cctcggccgc gtggaccagg cgctcacccg gtggaccacg acgctggcgc
     2821 cgttcaaggg cacgaaggtg gtggtggacc acaacatgtg gatctacttc ctcacccgct
     2881 tcggcctcgt cgaggcgggc tccatcgagg agcgccccgg catcccgccg acgccctcgc
     2941 acctgaccaa gctcatcgcc ctgatgaaag accagaaggt acgggtcatc ctctcggtgc
     3001 cctggggcga tcagaagatc gcggagcggg tggcccagga gacgggcgcg aaggtggtcc
     3061 cgctcgcgcc cgcggtcggc tccgccaagg gcaccgacgg ctacctggag acgatcgact
     3121 acaatgtccg gagcgtcgcg caggcgctca agtgatgccg actccgcaga agggacaccc
     3181 gacgagatga gcggcgatct gctcgccctg atgtggtttc cgttcctgat gtgcctggtg
     3241 ctcaccggca tccacgccta cctgggcttc cacgtgatcg cccgcgaggt ggtcttcgtc
     3301 gacatcgcgc tcgcccagat cgcggccctc ggcgccaccg ccgcgttcct gctgggctac
     3361 gagctcgaga cgtgggagtc gtacgcagcc ggcctcggct tcacggtgct gggcgccctc
     3421 gtgctggcgc tcacccgctc gcgcacgcgg cgcgtctcgc acgaggcggt gatcggggtg
     3481 gtctacgcgg tctcctccgc gggcgcggtc ctgctggccg accgctcgcc ccacggggcc
     3541 gagcacctgc gcgggatgct ggtgggctcc atcctgtccg tccgcggcga cgaggtcctc
     3601 aaggtcgcga tcctctacgc gctggtcggc ctcttccact ggctctgccg gcggcccttc
     3661 ttcctgatct ccaccgatcc cgaccgcgcc tatcgcgagg gctggcgcgt gcgggcgtgg
     3721 gacttcgcct tctacgcctc cttcggggtc gtcgtgacct cgtcggtgcg catcgcgggc
     3781 gtgctgctcg tcttctcgta cctcatcgtg cccgcgctgg ccggcattct cctggcctcc
     3841 cgcatcggcg cccgcctggc cgtcggctgg gccttcggcg ccggcgtcag catcatggcg
     3901 atggtcgcct ccgcggtgat cgacctgccc accggcgcca cggtggtctg cgccttcggc
     3961 ctgagcctct tcgtcctcgg gctcctcctg cgtctccgcc cgcggccgct cggagccgcc
     4021 gcgctggccg acccgggcgg cggccacgcg tgaccggccg tctccggggc atcgccctcg
     4081 cggcgatcct cgccctcgcc ggctgccagg aatcgcccgc gccggcgaag ccgcaggtga
     4141 tcgccaccat ctaccccctc tgggagctga cgcgccaggt ggcgggcgat cgcgccgagg
     4201 tgacctcgct cgtgccgccc ggcgtcgagc cgcacgactg ggagccctcg ccgcgcgacg
     4261 tctcgctcgt gcagcgggcc agcgtgatcg tccataccgg gaccggcctc gacgcctggg
     4321 ccgtgcggct cctggccgcc ctgcccgccc gcaccaccgt cgtgaacgcc gcgcgcggcc
     4381 tgagcctcct cccgcagggc ccgagcgtcg acccccacgt gtggctcgat cccacgctcg
     4441 cgagcgtcca ggcgcagacg atcgcggacg ggctcgcgca ggccgactcc gccgggcggc
     4501 ccgtctacct ggagaacgcg aaggcgctcg ccgcgcggct cggcgcgctc gaccaggcct
     4561 tcgcggcggg actgcgcgac tgcgcgcggc gcgaggtggt gaccacgcac gccgcgttcg
     4621 cctacctggc ccggcactac cagctcaccc aggtgccgct catggggagc gcccccgagg
     4681 ccgagccgag cccggcggac ctcgcggcga tcgtgcagac cgcgcggcgg ctgcacgtca
     4741 cccacgtctt ctt
//
LOCUS       DATMCV010000734         9679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_908583, whole genome shotgun sequence.
ACCESSION   DATMCV010000734 DATMCV010000000
VERSION     DATMCV010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908583"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1752)
                     /locus_tag="VNU03_16850"
     CDS             complement(1..1752)
                     /locus_tag="VNU03_16850"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP76194.1"
                     /translation="MRNVVIIGGGPAGLTAARELTKFGDRPTVLEKNPHVGGLSCTLE
                     YKGYHFDMGGHRFFTKVDEVNKIWQEVLGGEFLRRPRMSRIFYNDRFFHYPLKPLNAL
                     MGLGIIQAILVVLSYLRWQIWPHKQEDTFEQWVTNRFGHRLFSIFFKTYTEKVWGIPC
                     SELRAEWAAQRIKGLSLKTVLLTMFLKPQKTIKTLIEEFDYPRLGPGMMWQAVAAEVQ
                     EKGGQVRLGEDVVRICRTGSRIDSVVVARDGREETLPATDFISSMPVSELILKLSPPP
                     PEPVLDAARRLTYRDFLTVCLIVRKRDLFPDNWIYVHSAQVQVGRIQNFANWSPAMVP
                     DPSKSSLGLEYFCTEGDALWNTPDARLLELAGRELETIGLATRADVEDGCVFRVPKAY
                     PVYDASYAEYMVTVREFVDGLENLQTIGRNGLHRYNNQDHAMLTGLLAVRNLVLGEHN
                     DLWSVNTEAEYHEEITEKALVPALEEYFPRVDRVALAGATGAASGLALFLATILLVLK
                     GGAVVGPTLGLLGHYFPGYTVSTSGAVLGLLYGFVAGGALGWTFALLRNTVLLLYVAI
                     IRRRVEMGLLRRFLEFV"
     gene            complement(1749..2624)
                     /locus_tag="VNU03_16855"
     CDS             complement(1749..2624)
                     /locus_tag="VNU03_16855"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWP76195.1"
                     /translation="MPIERTLMFVGAHPDDESFGPGGTLAKYAGDGVRVVYACATRGE
                     AGGDETDDAAAHAQLGERRWSELRCAATALGLADVLHLGYRDSGMPGSPDNRHPRALV
                     TAPIEEVARDVVAVIRQVRPQVVITFDPIGGYYHPDHIAMHQATALAFHQAGRPDHFP
                     ALGAPWSPSKLYYWVPSRTLLRLAVRALRLLGRDPSRSGRNHDVDLASIATVDFPVHT
                     RIRLSRRVLARKHAAGACHQSQLPPGSLPRRAVRVLSRYLGSTELFTLAHPRPWNGLS
                     EIDLFDGVAAAKEPS"
     gene            complement(2603..3853)
                     /locus_tag="VNU03_16860"
     CDS             complement(2603..3853)
                     /locus_tag="VNU03_16860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP76196.1"
                     /translation="MTARTLHLVPRSAAGAVVFDPGNFTPYYVDALCAAFRRLGVRAQ
                     VISSPPLFETVDPAGRYEVARCFFPSLGGPLGRLLRRRTRLRQVVKALGYPLGLWRTW
                     RLLRTESPGVLHVQWALLPLLDGLLARALRARGWRVVYTVHDPLPEPDRLLAARHQRC
                     LLSQVDACIVHTPALERSLVASYPELTDRVRVIPHGGVPEPLPTEADRASARLRLGLA
                     PSGPLLLLFGMLKPYKGLEDLLMAMPAVLARFPRCRLLVAGEPLMPMAPIHQQIERLG
                     LQRAVTLRAAFVPQEQVAAYFTAADLILAPYREIAASGVVVTAQGHARAVLVTRVGGL
                     PEFVEPDNCGLVVPPRDPGALAEAICRALADPEELARMGQRARRRIAADHDWTDVARK
                     TLALYQVLPLTATPGAPACQSNAR"
     gene            complement(3859..4920)
                     /locus_tag="VNU03_16865"
     CDS             complement(3859..4920)
                     /locus_tag="VNU03_16865"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWP76197.1"
                     /translation="MIRLAFVGCGAVTEEEHLPALRGLPGVQVVAAVDPRPERLALVG
                     DRFAIPRRYAHVDALAADREVDAVGVCIPAGRHRLAVPTLLAAGKHVLVEKPPGLALE
                     DVDEMIAAARRSGAKAMVGYHMRWHRMVRQARALLDRGVLGPLESVRMVWYGPRDDQD
                     LPEWRARRELGGGALIETGVDHFDLWRHLVGADVDEIFARSRSGRRDDEAAVVSAVFD
                     SGVLGSAVFSERTTEDIEFEVCGAAGRLRVSCNRVEGLALHPAGADPASPRARLRRIP
                     EVLRLLPTALRNLRQGGDFKTSYRTQWEHFLDAIRSGGPVGCTLEDGRAALQTALAAV
                     ESAASGHPVRLSAATRRPA"
     gene            complement(4917..5960)
                     /locus_tag="VNU03_16870"
     CDS             complement(4917..5960)
                     /locus_tag="VNU03_16870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWP76198.1"
                     /translation="MALARPRLSVVVPVYNDARNLAECLAALRAADIPGCELIVVDDA
                     SPDDSADVARRAGAMVITLPQNSGPAAARNRGAAAATGEILFFVDADVAVASDAIRRV
                     ADAFAADPDLAALFGSYDDAPRAPGMVSQYRNLLHHWVHQQGNPEASTFWAGCGAVRR
                     ATFLEVGGFDAGLFRRPAIEDIELGYRMRRRGYRIRLDKDLQGKHFKGWTLYSVIKTD
                     ILSRAVPWSRLVLEGGGAPNDLNLKRGQRLSGLLVLLALAALVLSPLRPLLLGLSATA
                     LGAVVVLNWPLYRFLLARRGPWFLARALPLHWLYYLYSSLTYLAIWIHVHARRGARPM
                     PAADTPAPGRERA"
     gene            6090..7280
                     /locus_tag="VNU03_16875"
     CDS             6090..7280
                     /locus_tag="VNU03_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76199.1"
                     /translation="MLGSRFLSLMKRSLRAWGPLAPVIWALAGCAAEGPIESTAPVAD
                     VKVTPTMDTLRVGESVQLYADPRDARGRVLKGRSVAAVSQEPAVVNLLPGGVASALTP
                     GVARVLVTSEGQTATVSITVLAPAGPIAECESSKPAWLWCDDFERDRLDQYFEYSSAA
                     GGFVRAAGVGYGGSSGMRVRFQPKQVDVGSLHLAIGKTPSAYMRAVDAGTAVHREVYW
                     RFYVRYSPDWVGGGGNKVSRAQSLAATTFAQAMIAHVWSGSTPGDQVPIDRLGLDPWT
                     GVGWRGKLETSEYNDFPHLRGIGRDFSKTTLFDRVHTGKWQCIEAHARLNDPGRSNGL
                     FELWLADQPEAKLTGLGWIGSFRDFGINAIYLENYWNDGAPQAQERYFDNFVVSTERI
                     GCLK"
     gene            complement(7285..8892)
                     /locus_tag="VNU03_16880"
     CDS             complement(7285..8892)
                     /locus_tag="VNU03_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWP76200.1"
                     /translation="MTANQLEPAYDYVIVGGGPAACVLANRLSADPSTRVLLLEAGTG
                     NSSMIRRLLARFTNWFDPELRWQYETVPQPGMNGRRVYLPQGRTLGGGSAINAMTYVR
                     GQPADFEAWVRLGNDGWDYATVLEAYKRLEKNAVLRDEFHSQAGRLSVIDQVHPHRLT
                     QVFVEAGRQVGLPFNRDFNGQSQLGVGTYQVTQTNGKRCSAADAFLHPVRHRANLHVR
                     THAYVTRVCLEGSRATGVEFRAGGRPHRARAEREVIVSGGAINSPKLLLLSGIGPADD
                     LRRLGITVAHDLPGVGKNLHDQLNVQVITRASRPITYDGWDRPVPFLRYALQFALFNS
                     GVATSNICEGAAFLPGDPGLDAPDIQLHFMPLLWLNYGRTHIPGHGMTLEAAFLQPLG
                     RGSVTLASADPSVSPLIDPAYGACPEDIHGLVQAIRRSREIMQAPAFRPFTDGELYPG
                     RDKQSDAELADYARQSAITTYHPVGTCRMGVDALAVVDPRLRVRGIEGLRVVDSSIMP
                     RTTSGSTQAPSGMIGEMGAAMILKASA"
     gene            9100..9175
                     /locus_tag="VNU03_16885"
     tRNA            9100..9175
                     /locus_tag="VNU03_16885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9133..9135,aa:Val,seq:cac)
     gene            9286..>9679
                     /locus_tag="VNU03_16890"
     CDS             9286..>9679
                     /locus_tag="VNU03_16890"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP76201.1"
                     /translation="MIDESAVDRRISPAERMLGASGREHYFRVGQSALECIQKALDIA
                     SISPHRIKRILDLPCGHGRVLRYLKAAFPEAEITACDLLRDGVDYCSSMFGATALYSC
                     DDPEDIPLHPGSFDLIWVGSLLTHLDAAL"
BASE COUNT         1537 a         3217 c         3541 g         1384 t
ORIGIN      
        1 tcacacgaac tcgaggaagc gccgcagcag gcccatctcg acgcgccgcc ggatgatcgc
       61 cacatagagc agcagcacgg tgttacgcag cagggcgaac gtccagccga gcgccccacc
      121 cgccacgaac ccgtacagca ggccaaggac ggcgcccgac gtgctcacag tgtagccggg
      181 gaagtagtgg ccgagcaatc cgagggtggg gcccacgacc gcgccgcctt tgagcacgag
      241 caggatcgtg gccaggaaga gggccagccc ggacgccgcg ccggtggccc cggcaagggc
      301 cacgcggtcg acgcggggga agtactcctc gagggcgggg acgagggcct tctcggtgat
      361 ctcctcgtgg tactcggcct cggtgttcac gctccagaga tcgttgtgct cgccgaggac
      421 caggttgcgc accgcgagga gcccggtgag catcgcgtga tcctggttgt tgtagcggtg
      481 caggccgttg cgcccgatgg tctggaggtt ctccaggccg tcgacgaact cgcgcaccgt
      541 gaccatgtac tccgcgtagg aggcgtcgta gaccgggtat gccttgggca cccggaagac
      601 gcacccgtcc tcgacgtcgg cccgggtcgc gaggccgatg gtctcgagct ctcgaccggc
      661 cagctcgagg aggcgcgcgt ccggggtgtt ccagagcgcg tcgccctcgg tgcagaagta
      721 ttcgaggccc aggctgctct tcgaggggtc gggcaccatg gccgggctcc agttggcgaa
      781 gttctggatg cggccaacct gcacctgggc cgagtgcacg tagatccagt tgtccgggaa
      841 gagatcgcgc ttgcgcacga tcaggcacac ggtcaggaag tcgcgatagg tcaggcggcg
      901 ggccgcgtcc agcaccggct ccggaggcgg cggcgagagc ttcaggatca gctcggacac
      961 cggcatgctc gagatgaagt cggtggcggg cagcgtctcc tcgcgcccgt cgcgggcgac
     1021 caccaccgag tcgatgcggg agccggtacg gcagatccgc accacgtcct cgcccaggcg
     1081 cacctgcccg cccttctcct ggacctcggc cgccaccgcc tgccacatca tgccagggcc
     1141 caggcgcggg tagtcgaact cctcgatcag ggtcttgatg gtcttctggg gcttgaggaa
     1201 catggtcagg agcacggtct tgagcgagag ccccttgatc cgctgggccg cccactcggc
     1261 gcgcagctcg gagcagggga tgccccagac cttctcggtg taggtcttga agaagatcga
     1321 gaagaggcgg tgcccgaagc ggttggtcac ccactgctcg aaggtgtcct cctgcttgtg
     1381 gggccagatc tgccagcgca gatagctcag caccaccagg atcgcctgga tgatcccgag
     1441 ccccatcagg gcgttgagcg gcttgagcgg gtagtggaag aagcggtcgt tgtagaagat
     1501 ccgcgacatg cggggccggc gcaggaactc gcctccgagc acctcctgcc agatcttgtt
     1561 cacctcgtcc accttggtga agaagcggtg gccgcccatg tcgaagtgat agcccttgta
     1621 ctcgagcgtg caggaaagcc cgcccacgtg cgggttcttc tcgagcacgg tgggccggtc
     1681 cccgaacttg gtcagctcgc gggcggcggt gaggcccgcg ggtccgccgc cgatgatcac
     1741 gacattcctc atgatggttc ctttgcggcc gccacaccgt cgaagaggtc gatctccgac
     1801 agcccgttcc agggccgggg gtgggcgaga gtgaacagct cggtcgagcc gagataccgc
     1861 gagagcaccc ggaccgcgcg gcgcggcagg gatccaggcg gcagctggct ttggtggcac
     1921 gcgcccgccg cgtgcttccg ggcgaggacg cggcgggaga ggcggatgcg ggtgtgcacc
     1981 ggaaagtcca ccgtcgcgat gctcgcgagg tcgacgtcgt ggttgcggcc gctccggctc
     2041 ggatcccgcc cgaggaggcg gagggcgcgg acggccaggc gtaacagggt gcgggacggg
     2101 acccagtagt agagcttcga cgggctccag ggcgcgccga gggccgggaa gtggtccggc
     2161 cggcccgcct ggtggaacgc gagcgcagtc gcctggtgca tcgcgatatg gtccgggtgg
     2221 tagtagccgc cgatcggatc gaaggtgatc accacctgcg gccgcacctg ccggatcacc
     2281 gcgaccacgt cccgcgcgac ctcctcgatc ggcgcggtca cgagggcccg cggatgccgg
     2341 ttgtcgggcg acccgggcat gccggagtcg cgatagccca ggtggaggac gtcggccagc
     2401 ccgagcgcgg tggccgcgca gcgcagctcg ctccagcggc gctcgccgag ctgggcgtgc
     2461 gcggccgcat cgtccgtctc gtcgcccccc gcctcgcccc gcgtggcgca ggcgtagacc
     2521 acccgcaccc cgtcgccggc gtacttggcg agcgtgccgc cggggccgaa cgactcgtcg
     2581 tccgggtgcg cgcccacgaa catcagcgtg cgttcgattg gcatgcgggg gcacccgggg
     2641 tcgcggtgag cggcaggacc tggtagaggg cgagcgtctt gcgggccacg tccgtccaat
     2701 cgtgatccgc cgcgatgcgc cggcgcgcgc gctggcccat gcgggccagc tcctccgggt
     2761 cggcgagcgc gcggcagatc gcctccgcga gggcgccggg atcgcggggc gggaccacga
     2821 gcccgcaatt gtccggctcg acgaactccg ggaggccgcc gacccgggtc accagcacgg
     2881 cgcgcgcgtg accctgggcg gtgaccacaa ctccgctcgc cgcgatctcg cgatagggcg
     2941 ccaggatgag gtcggcggcg gtgaagtagg cggccacctg ctcctgcggg acgaaggccg
     3001 cccgcagggt caccgcgcgc tggaggccga gacgctcgat ctgctggtgg atgggcgcca
     3061 tcggcatgag gggctcgccg gccaccagca ggcggcagcg cgggaaccgc gccaggacgg
     3121 ccggcatcgc catcaggaga tcctcgaggc ccttgtacgg cttgagcatc ccgaagagca
     3181 ggagcagcgg cccgctcggc gccaggccca ggcgtagccg cgcgctggcg cggtccgcct
     3241 cggtgggcaa gggctcgggc acgccgccgt gcgggatcac ccgcacgcgg tcggtcagct
     3301 cgggatagga ggccaccagc gatcgctcga gcgccggggt gtggacgatg caggcgtcca
     3361 cctgcgagag caggcagcgc tggtggcggg ccgccagcag gcgatcgggc tccggcagcg
     3421 ggtcgtgcac ggtgtagacc acgcgccagc cgcgggcccg cagggcccgg gcgagcaggc
     3481 cgtccaggag cgggagcagc gcccactgca cgtggagcac gcccggcgac tcggtgcgca
     3541 gcagtcgcca cgtgcgccag aggcccaggg gatagccgag cgccttgacg acctggcgca
     3601 gccgcgtgcg acggcgcagc aggcgcccga gggggccgcc cagcgacgga aagaagcagc
     3661 gcgcgacctc gtagcgcccg gccgggtcga ccgtctcgaa gagcggcggc gaggagatga
     3721 cctgcgcgcg cacgccgagc cggcggaagg cggcgcagag cgcgtcgacg tagtagggcg
     3781 tgaagttgcc cgggtcgaac acgaccgcgc ccgcggcgct gcgcggcacg agatggagcg
     3841 tccgggcggt catgtccgtt acgctgggcg ccgcgtcgcc gcgctcaggc gaaccgggtg
     3901 gccgctcgcg gccgactcca ccgcggccag cgcggtctgg agcgcggcgc ggccatcctc
     3961 gagcgtgcag cccaccgggc cgccgctacg gatcgcgtcg aggaagtgct cccactgggt
     4021 gcggtacgag gtcttgaagt cgccgccctg ccgcaagttc cgcagcgcgg tgggcaggag
     4081 gcgcaggacc tccgggatgc ggcggagccg ggcgcgcggg ctcgccgggt ccgcgccggc
     4141 gggatggagc gcgaggccct cgacacgatt gcaggagacg cgcagccggc cggccgcgcc
     4201 gcagacctcg aactcgatgt cctcggtggt gcgctcggag aagacggccg agccgagcac
     4261 cccgctgtcg aagaccgcgc tcaccaccgc ggcctcgtcg tcacgccggc ccgagcggct
     4321 gcgggcgaag atctcgtcga cgtcggcgcc aaccagatgg cgccagaggt cgaagtggtc
     4381 caccccggtc tcgatgagtg cgccgccgcc cagctctcgg cgcgcgcgcc actccgggag
     4441 gtcttggtcg tcgcgcggcc cgtaccagac catgcgcacg ctctcgaggg gaccgagcac
     4501 gccccggtcc aggagcgcgc gggcctggcg aaccatgcgg tgccagcgca tgtggtagcc
     4561 gaccatggcc ttcgcgccgg accggcgggc cgcggcgatc atctcgtcga cgtcctcgag
     4621 ggcgagcccg ggcggcttct cgaccagcac gtgcttgccg gcggcgagca gcgtcgggac
     4681 cgcgaggcga tggcggccgg cggggatgca gacgcccacc gcgtcgacct cgcggtcggc
     4741 ggcgagcgcg tcgacgtgcg cgtagcgccg ggggatcgcg aagcggtcgc ccacgagcgc
     4801 gagccgctcg ggtcgcggat cgaccgccgc caccacctgg acgccgggca gaccgcgcag
     4861 cgccgggaga tgctcctcct cggtgaccgc gccgcagccg acgaaggcca ggcggatcat
     4921 gcccgctcgc gtcccggcgc cggggtgtcg gcggcgggca tggggcgggc gccgcgccgg
     4981 gcgtgcacgt gaatccatat cgccaggtag gtcaggctgc tgtagaggta gtagagccag
     5041 tgcagcggca gcgcgcgggc gagaaaccac ggcccgcgcc gggcgaggag gaagcgatag
     5101 agcggccagt tcagcacgac gaccgcgccg agcgccgtcg cgctcaggcc gagcaggagc
     5161 ggacggagcg gggagaggac gagagcggcc agggccagca gcaccaggag cccggagaga
     5221 cgctgcccgc gcttgaggtt gagatcgttg ggcgccccgc cgccctcgag cacgagccgc
     5281 gaccacggca ccgcgcgcga caggatatcg gtcttgatca ccgagtagag cgtccagccc
     5341 ttgaagtgct tgccctgaag gtccttgtcg aggcgaatgc gatagccgcg ccggcgcatc
     5401 cggtagccca gctcgatgtc ctcgatggcc gggcgccgga agagacccgc gtcgaagccg
     5461 ccgacctcga ggaaggtcgc ccggcgcacc gcgccgcagc cggcccagaa ggtggacgcc
     5521 tccggattgc cctgctggtg gacccagtgg tggagcaggt tgcgatactg cgagaccatg
     5581 ccgggggccc gcggggcgtc gtcgtacgag ccgaagaggg ccgcgaggtc gggatcggcc
     5641 gcgaaggcgt cggccacgcg gcggatcgca tccgaggcga ccgcaacgtc ggcatcgacg
     5701 aagaacagga tctcgccggt ggcagccgcc gcgccccgat tccgggccgc cgcggggccc
     5761 gagttctgcg gaagggtgat caccatggcg cccgcgcgac gggctacgtc agccgagtcg
     5821 tccggcgagg cgtcgtcgac cacgatgagc tcgcagcccg ggatgtcggc cgcccggagt
     5881 gcggcgagac attccgcgag gttacgggca tcgttgtaca cgggtaccac gacggacagg
     5941 cgaggccgag cgagcgccat gggctgttag gggggcatat tccatgccga ccacggggaa
     6001 gagccatggg cccaactcgt caagcttact gcggggttgc acggcgcgcc cgtcggcgag
     6061 actaaatcgt gcagagaaag gaaatcgcca tgttgggtag tcggtttctg agcctgatga
     6121 agcggtcgct gcgggcctgg ggccccctgg ccccggtgat ctgggcactg gcgggctgtg
     6181 ccgccgaggg cccgatcgag tccaccgcgc cggtggccga cgtgaaggta acgccgacca
     6241 tggacaccct gcgggtgggg gaaagtgtgc agctctatgc cgacccgcgc gacgcgcgcg
     6301 gacgggtcct caagggccgg tcggtcgctg cggtgagcca ggagccggcc gtggtgaacc
     6361 tgctgcccgg aggtgtcgcg agcgccctga ccccgggcgt cgcccgggta cttgtcacca
     6421 gcgaagggca gacggccacg gtgtccatca ccgtgctggc gccggctggg ccgatcgcgg
     6481 agtgcgagtc gtccaagccc gcctggctct ggtgcgatga cttcgagcgg gaccgcctcg
     6541 atcagtactt cgagtactct tcggccgccg gcggcttcgt ccgggcggcg ggcgtgggct
     6601 acggagggtc ctccgggatg cgggtgcggt tccagccaaa acaggtagac gtgggcagtc
     6661 ttcacctggc catcggcaag acgccatctg catacatgcg tgcagtggac gccggcaccg
     6721 ccgtgcatcg tgaggtgtac tggcgcttct acgtgcgcta ctcacccgat tgggtgggcg
     6781 gcggcggcaa caaggtgtcg cgcgcccaga gcctcgcggc cacgaccttt gctcaggcca
     6841 tgatcgcgca cgtctggtcg ggcagcactc ccggagacca ggtgcccatc gaccggcttg
     6901 gcctcgaccc ctggaccggg gtcggctggc ggggaaagct cgagaccagc gagtacaacg
     6961 acttccctca tttgcgtggg atcgggcggg acttctcgaa gaccacgctc ttcgaccgcg
     7021 tccacaccgg aaagtggcag tgcatcgagg cccacgcgcg gctcaacgat cctggccgga
     7081 gcaacggact cttcgagctg tggctcgcgg accagcccga ggccaagctc acgggcctcg
     7141 gctggatcgg gtcctttcgg gacttcggca tcaacgcgat ctatctcgag aactactgga
     7201 acgacggtgc cccccaggcc caggagcgct acttcgacaa cttcgtggtg agcaccgaac
     7261 ggatcggctg cctgaagtga gcggctaagc ggaggccttc aggatcatcg cggctcccat
     7321 ctccccgatc attcccgacg gggcctgggt gctgccactc gtagtgcgcg gcatgatcga
     7381 cgagtccacg acgcgcaggc cctcgatccc gcgcacccgg agccgcggat cgaccaccgc
     7441 cagggcatcg acccccatcc ggcaggtgcc gaccgggtgg tacgtggtga tggcgctctg
     7501 ccgcgcatag tcggcgagct ccgcatccga ctgcttgtcg cgaccggggt agagctcccc
     7561 gtcggtgaac gggcggaacg cgggcgcctg catgatctcg cgcgagcggc ggatcgcctg
     7621 cacgagcccg tggatgtcct cggggcaggc accataggcc ggatcgatga gcggggaaac
     7681 gctcggatcg gcggaggcga gagtcacgct gccgcggccg agcggctgga gaaaggcggc
     7741 ctcgagcgtc atcccgtggc cgggaatatg ggtgcggccg tagttaagcc agagcaaggg
     7801 catgaagtgg agctggatgt ccggcgcgtc cagcccgggg tcgccgggaa ggaaggcggc
     7861 tccctcgcag atgtttgacg tagcgacgcc cgaattgaag agcgcgaact gcagagcata
     7921 ccggaggaag ggaactggcc gatcccagcc gtcgtaggtg atcggcctgc tggcacgggt
     7981 gatcacctgg acgttcagct gatcgtgcag gttcttgccg acgccgggca ggtcgtgggc
     8041 gaccgtgatc ccgagacgcc gcaggtcatc ggccgggccg atccccgaca gcaggagcag
     8101 cttgggcgag ttgatcgccc ccccgctgac gatgacttcg cgctcggccc gcgcccggtg
     8161 gggccggccg ccagcccgga actcgacgcc agtggcccgg ctcccctcga ggcagacgcg
     8221 cgtcacgtag gcgtgcgtgc ggacgtgcag gttcgcgcgg tgccgcaccg gatgcaggaa
     8281 ggcatcggcc gcgctgcagc gcttgccgtt ggtctgggtt acctggtagg tgcccacgcc
     8341 cagctgggac tggccattga agtcacggtt gaagggcagc cccacctgtc ggcccgcctc
     8401 cacgaacacc tgggtgagcc ggtgcgggtg cacctgatcg atcacgctca gccgcccggc
     8461 ctggctgtga aactcgtctc gcagcaccgc attcttctcg agccgcttgt aggcctcgag
     8521 cacggtcgcg taatcccacc cgtcattgcc gagccggacc caggcctcga agtcggcggg
     8581 ctgcccgcgc acgtacgtca tcgcgttgat cgcgctcccg ccgcccaggg tgcggccctg
     8641 cggcaggtag acgcggcggc cgttcatgcc gggctgcggc accgtctcgt actgccaccg
     8701 cagctcgggg tcgaaccagt tcgtgaaccg ggcgaggagc cgtcggatca tcgaggagtt
     8761 gccggtcccg gcttcgagca gcagcacgcg agtcgacggg tccgcactca ggcggttggc
     8821 gagcacgcat gcggccggcc cgccaccaac gatcacgtag tcgtaggcgg gctccaactg
     8881 attcgcggtc acggctccgc aatttaccac acgtgtgttt tcgctgggcc cgcacggcat
     8941 gcgtggaagt acataccaag gatgatcgac aggacctatt ccgcgaggta ggttggctca
     9001 gctctacgat caccggcggc aatccatcgg cgcatttctg ggcacaccga gggtgttcgg
     9061 cttgaaacct agtccgtaac cagctaatat tccgggcacg gacgattagc tcagttggca
     9121 gagcaccggc ctcacatgcc gggggtcact ggttcaagtc cagtatcgtc caccatcgaa
     9181 cgcaaaagac ccgaggggtt acatgcccct cgggtttcgt gctctgtggc tgtcgtgagc
     9241 cagaggaggg agtgtggccg cggccaacac caaggaggca acgaagtgat tgacgaatct
     9301 gccgtcgata gacgtatttc gcccgctgag cgcatgttgg gcgcaagcgg ccgggagcac
     9361 tactttcgtg ttgggcagtc ggcactggaa tgcatccaga aggcactaga tatcgcgtcc
     9421 atttcgccgc accgcatcaa gcgcattctt gatttgccat gtggtcatgg tcgagtgctg
     9481 agatacctga aggcggcgtt tcccgaggcc gaaatcactg cctgtgatct cctgcgcgac
     9541 ggagtggact actgttcctc gatgtttggg gcgacggcct tgtattcgtg tgacgacccc
     9601 gaggacatcc cgctgcaccc ggggagcttt gacctcattt gggttggttc cctcctcacc
     9661 catctggatg cagcactct
//
LOCUS       DATMCV010000735         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_174108, whole genome shotgun sequence.
ACCESSION   DATMCV010000735 DATMCV010000000
VERSION     DATMCV010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174108"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VNU03_16895"
     CDS             complement(<1..167)
                     /locus_tag="VNU03_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP76202.1"
                     /translation="METVTARTTNQPARVRYRDARVAGLDIFYREAGPKDAPTLLLLH
                     GFPTSSQMFRNL"
     gene            437..640
                     /locus_tag="VNU03_16900"
     CDS             437..640
                     /locus_tag="VNU03_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76203.1"
                     /translation="MLSTVACSSTTRLSSANMCKAAGGTYSQGTCQPGGNPLSAQKMC
                     EAHGGTYFSGGDYCEVGGSGWKY"
     gene            complement(794..1708)
                     /locus_tag="VNU03_16905"
     CDS             complement(794..1708)
                     /locus_tag="VNU03_16905"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HWP76204.1"
                     /translation="MIATRSAEDLLPALRLAHPEWRPLLALVEEALREIERPEWRQSV
                     PAPRDPQSEPLLSGAILTVGRRPIERWVRRVLETAAGIGPAAPFVDPAATRQVDPLAL
                     LQAAVAQDLARLDDIASAAGDTHGVLRGVAPLIAMPLLQACRRAWADPAPATWAHGYC
                     PTCGAWPALAEVRGLDGARHLRCRGCGGNWATSWLRCPFCGEHDHTRLGSLVASEGLE
                     REKIEVCDACHGYLKTITTFGPIPAEQVVLHDLATVGLDVAAVERGYRSPSPRSPLPV
                     RIVAGRSGLYRMTSTASAVVRTAFAKKP"
     gene            complement(1713..1928)
                     /locus_tag="VNU03_16910"
     CDS             complement(1713..1928)
                     /locus_tag="VNU03_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76205.1"
                     /translation="MALLVAGLAVACTSPESTRSRGGGPGADVGNHPRGPVELHAGAR
                     MFAGTPTSGKTIGRHALIGGTEQAGQR"
BASE COUNT          309 a          725 c          794 g          281 t
ORIGIN      
        1 agattgcgga acatctgcga gctggtcggg aagccgtgca ggagcaggag cgtcggcgcg
       61 tccttcggac cggcctcgcg gtagaagatg tccaggccgg cgactcgggc gtccctgtac
      121 cggactctcg cgggttggtt ggtcgttctc gcggtgacgg tctccatggc gtccttctcc
      181 tcgcgtgggt gggataccga cggctacccg tacgtgtggg agggtgcggc gacgacaccg
      241 gtgacgcctc ggaaggcttt cggcaggaat ccaacaggcc cgcttgccgc ttcttgcgga
      301 aagccgggcg gtcgcgagcg gtcgcgccgt gggtgggatc acaagcgcgg atcgaccaac
      361 caccacctca cgggaagggg acagacttat gagttcagta accgtacttc ggatgggggc
      421 gctggccggg cttctgatgc tctcgacggt cgcctgttcc agcaccacgc ggctgagcag
      481 cgccaacatg tgcaaggccg ccggcgggac gtactcgcag gggacgtgcc agccgggcgg
      541 caacccgctc agcgcgcaga agatgtgcga ggctcacggc ggaacgtact tctcgggcgg
      601 ggactactgc gaagtgggcg ggagcggctg gaagtactga caccgcgggc agccggccga
      661 ccggcgcgtc accggccgtc agggtggggg gacgtgccgg gtcggcttgt tgcggagctc
      721 gcgcacgttg ccgggtcagt cctagcgcgc gagctggcgc aggtgctcct gcgtcagctg
      781 cggcggtcgc cagctacggt ttcttggcga acgcagtcct gacgaccgcc gaggcggtgg
      841 aggtcatgcg gtagagtccg gagcgcccgg cgacgatccg gaccggcagc gggctccggg
      901 gactcggtga ccggtagccg cgctccaccg cggccacgtc gagccccacg gtggcgaggt
      961 cgtgcagcac cacctgctcg gccgggatcg ggccgaaggt ggtgatggtc ttgaggtatc
     1021 cgtggcaggc gtcgcagacc tcgatcttct cgcgctcgag gccctccgac gccaccaggg
     1081 agccgagccg cgtgtggtcg tgctcaccgc agaaggggca gcgaagccac gaggtcgccc
     1141 agttcccgcc acagccccga caccggagat gccgggcccc gtcgaggccg cggacctcgg
     1201 cgagcgcggg ccacgccccg caggtcgggc agtagccgtg cgcccaggtc gcgggcgccg
     1261 gatccgccca ggcgcggcgg caggcctgaa gcagcggcat ggcgatcagc ggagccactc
     1321 cccggagcac tccgtgagtg tcgcccgcgg cgctcgcgat gtcgtcgagg cgcgcgaggt
     1381 cctgggccac cgcggcctga agcaaggcga gcgggtcgac ctgtcgcgtg gcggccgggt
     1441 cgacgaaggg cgccgccggc ccgataccgg ccgcggtctc gagcacccga cgcacccacc
     1501 gctcgatcgg tcggcgaccc accgtgagga tcgcccccga cagcaggggc tcgctctgtg
     1561 gatcgcgcgg cgcgggcacc gactgccgcc actccgggcg ctcgatctcc cggagcgctt
     1621 cctcgaccag cgcgagcagc ggccgccact cggggtgcgc gagacgcagc gcagggagga
     1681 gatcctcggc cgaccgggtc gcgatcatgc gcctatcgct gcccggcctg ctcggtgccc
     1741 ccgatgaggg cgtgccggcc gatcgtcttg ccggaggtgg gtgtgccggc gaacatgcgg
     1801 gcgccggcat gcagctcgac cggcccgcgc ggatgattgc cgacgtcggc gccgggtccg
     1861 ccgcctcgac tccgggtcga ctcggggctg gtgcaggcga cggccagccc ggccacgagc
     1921 agtgccacga ccccgcgcca cctccaccgg ctcatagccg atccgaggag aagatgatga
     1981 ccatgcgtag catgagccct ccgagcagga cgagcgtggg cccgatccag gccggccgac
     2041 ccgccggccc tcgccagtcg aggaccaggg gcagcgcgat gccgcccagg accatcgcgg
     2101 ccaggagca
//
LOCUS       DATMCV010000736         4214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_573684, whole genome shotgun sequence.
ACCESSION   DATMCV010000736 DATMCV010000000
VERSION     DATMCV010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573684"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VNU03_16915"
     CDS             complement(<1..644)
                     /locus_tag="VNU03_16915"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWP76206.1"
                     /translation="MTRRGLVVLLLLLAASPLPAHAQEAERLEPVVVTATRAEQPIGE
                     APASVTVLSREDIRQGAGQSLDDILRQVPGFSLFRRSSSLVSHPTTQGVSLRGIGPSG
                     ASRALVLLDGVPVNDPFGGWVYWDRIPLQSVEQVEVVRGGGASLWGNYALGGVINIIT
                     RRPTERAAYFEGSYGTNDTHNVDLLLTDALGPFRVSLEGSHFATDGYPVVSSARR"
     gene            723..2375
                     /locus_tag="VNU03_16920"
     CDS             723..2375
                     /locus_tag="VNU03_16920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWP76207.1"
                     /translation="MDRIWQAHWPPGVDEARIRLPDDSLPARLKATAARLPRKTALVF
                     YGREISFTELDEASDRFAAWLRGRGVGPGDRVALYLENCPQFAVAHFGALKVGAITVA
                     LNPMHKAHELGHELEDSGARVIVAADAGREVVEGVRDRTALDTAVLVSYRDFLPEEPT
                     LPLPPSLLEEAPRRPRGWEDFQAIVGSAATLRALEPRARHDTALIQYTSGTTGAPKGA
                     ELTHGNVTANCELIRAFFGIGPADVLLGVVPWFHITGMEVQLNLMAYTGATLVALHRF
                     DVETALRAIQRYRCTVTTLIATINVAILNHEATPSHDLTSLRLCTSGGAPVPAEIARR
                     WEATTGHALVEGYGLTETTAPTHSNPPHRPRYGTVGVPLPYTGVRIVSLEDGVTEVAP
                     GESGEIAVRGPMVTKGYWGRPEATAEAIPDGWLRTGDIGRVDEEGYFTIEERKKDLIK
                     ASGYSVFPAEVEAIMYRHPAVAEVGVVGVPDPYRGEDVLAFVVRRTGATATERELVEW
                     CRAEMAVYKAPRTVRFVPDLPKTGSGKIVKRALREQARAGSG"
     gene            complement(2412..2882)
                     /locus_tag="VNU03_16925"
     CDS             complement(2412..2882)
                     /locus_tag="VNU03_16925"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP76208.1"
                     /translation="MATSAHREEIWVPGMPEPISHFVHVVRAGRLVFVSGCVASDDHG
                     QTVGGSDIVAQTRQVHENIKRCLAAAGATFADVCKVTVYLKNVGDREKVNSVRKEYFG
                     ASRPASTLIEISQFVRPDLLIEVEAVAVLPERSAAKPGRRKPARRAAPRKRRRR"
     gene            3091..3510
                     /locus_tag="VNU03_16930"
     CDS             3091..3510
                     /locus_tag="VNU03_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76209.1"
                     /translation="MISRLATAALAALLLLICSAAPTHSQALDPASADALATTLRMLA
                     DPATRGRIIASDPSAAEIDRQVQGMAGSSALVQEVYRLAAEVFADLTRNSNGDPRAMS
                     AALDRAKSDPAGFAAMLRPETLERLRALSIKISDTQR"
     gene            3519..4199
                     /locus_tag="VNU03_16935"
     CDS             3519..4199
                     /locus_tag="VNU03_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76210.1"
                     /translation="MTTRMTEDPISLLKGRMAEALVEAVFKRAKYTVARVGRETQMPA
                     LVRSGRTEFMPDFMVWRALDGPSLGHHSYRLVAIEVKYRADLADYLCRDSPSRSAEVA
                     QQWPELYEILVTDRPEVGRSCFQAIHVSEVPAGTVPITLDLHQVSGLDIYRRTVEEYE
                     GLVREIFPILGSRQGSGQGCVEPERKPLARMLGRNQGRGHRLTRPARDPHRYRPSVVS
                     EHRPGASE"
BASE COUNT          592 a         1545 c         1494 g          583 t
ORIGIN      
        1 cggcgcgccg agctcaccac cgggtagccg tcggtggcga agtggctgcc ctcgaggctc
       61 acgcggaagg gtccgagcgc gtcggtcagg agcaggtcca cgttgtgggt gtcgttggtg
      121 ccgtagctcc cctcgaagta ggcggcgcgc tcggtgggcc ggcgggtgat gatgttgatt
      181 accccgccga gggcgtagtt gccccagagg ctcgcgcccc cgccgcggac gacctccacc
      241 tgctccacgc tctggagcgg gatccggtcc cagtacaccc agcctccgaa cggatcgttg
      301 accggcaccc cgtcgagcag cacgagggcg cggctcgccc cgctgggccc gatgccgcgc
      361 agcgagacgc cctgcgtggt cgggtgggag acgaggctcg aggagcggcg gaaaaggctg
      421 aagccgggca cctgacgcag gatgtcgtcg aggctctgcc ccgccccctg gcggatgtcc
      481 tcgcgggaga gcacggtcac gctggccggc gcctcgccga tcggctgctc ggcgcgggtc
      541 gcggtgacca ccaccggctc gaggcgctcg gcctcctgcg cgtgcgcggg aagcggcgag
      601 gccgcgagca ggaggagcag gacgacgagg ccccggcgcg tcatcgtggg ccaggatgct
      661 acgcgccgat ccacggagtg tcaacgcagg agggcgtcga ctgcggtgtt agtatccgcc
      721 ccgtggatcg gatctggcag gcgcactggc cccccggcgt cgacgaggcg cgcatccggc
      781 ttcccgacga ctcgctgccc gcgcggctca aggccaccgc cgcgcggctc ccgcgcaaga
      841 cggcgctggt tttctacggg cgggagatct cgttcacgga gctggacgag gcgagcgacc
      901 ggttcgcggc ctggctccgt ggacggggcg tcggtccggg agaccgggtc gcgctctacc
      961 tcgagaactg cccccagttc gcggtggccc acttcggcgc gctcaaggtg ggcgccatca
     1021 ccgtcgcgct caatccgatg cacaaggccc acgagctggg gcacgagctg gaagattcgg
     1081 gggcgcgcgt catcgtggcg gccgacgcgg gccgcgaggt cgtggagggc gtacgcgacc
     1141 ggaccgccct cgacaccgcg gtgctcgtct cctaccgcga cttcctgccg gaggagccca
     1201 ccctgcccct tccgccgtcg ctgctcgagg aagcgccccg gcggccgcgc ggctgggagg
     1261 acttccaggc catcgtcggg agcgcggcca ccctccgcgc gctcgagccg cgggcgcgtc
     1321 acgacaccgc gctgatccag tacacctccg gcaccaccgg cgcgcccaag ggcgccgagc
     1381 tgacccacgg caacgtcacc gccaactgcg agctgatccg cgccttcttc ggcatcgggc
     1441 ccgccgacgt gctcctgggt gtcgtgccgt ggttccacat caccggcatg gaggtacagc
     1501 tcaacctgat ggcctacacc ggggcgacgc tggtggcgct ccatcgcttc gacgtggaga
     1561 ccgcgctgcg cgccatccag cgctaccggt gcacggtcac caccctcatc gcgacgatca
     1621 acgtggccat cctcaaccac gaggcgacgc cgagccacga tctcacctcg ctccgtctgt
     1681 gcacctcggg cggcgcgccg gtgcccgcgg agatcgcgcg gcgctgggaa gcgaccaccg
     1741 gtcatgccct ggtggagggc tacgggctca ccgagaccac cgcgcccacc cattccaacc
     1801 cgccccaccg cccgcgctac gggaccgtcg gggtgccgct gccgtacacc ggggtgcgca
     1861 tcgtgagcct cgaggacggc gtgaccgagg tggcgccggg cgagtcgggc gagatcgcgg
     1921 tccgcgggcc gatggtcacc aagggctact ggggccgacc ggaggcgacc gcggaggcca
     1981 tcccggacgg ctggctccgg accggcgaca tcggccgggt ggacgaggag ggctacttca
     2041 cgatcgagga gcgcaagaag gacctgatca aggcctccgg ctacagcgtc ttcccggcgg
     2101 aggtggaggc catcatgtac cggcaccccg cggtggcgga ggtcggggtg gtgggggtgc
     2161 ccgatcccta ccgcggcgag gacgtgctcg ccttcgtggt ccggcggacg ggcgcgaccg
     2221 cgaccgagcg cgagctggtc gagtggtgcc gcgccgagat ggcggtctac aaggcgccgc
     2281 gcacggtgcg cttcgtgccc gacctgccca agaccggctc gggcaagatc gtcaagcgcg
     2341 ccctgcgcga gcaggcccgc gccggctcgg gctgaggcgt gcggaccgac gctccgccct
     2401 gctccctcgg ctcagcggcg ccggcgcttc cggggcgcgg cacgccgtgc gggcttccgc
     2461 cggccgggct tcgccgcgct ccgctcgggg agcaccgcca ccgcctcgac ctcgatcaac
     2521 aaatcaggcc gcacgaactg ggagatctcg atgagggtgc tggccgggcg gctcgccccg
     2581 aagtactcct tccgcaccga gttgaccttc tcgcggtcgc ccacgttctt aaggtacacc
     2641 gtcaccttgc agacgtcggc gaacgtcgcc cccgccgcgg ccaggcagcg cttgatgttc
     2701 tcgtggacct gccgggtctg ggccacgatg tcgctgccgc ccaccgtctg cccgtggtcg
     2761 tcgctggcca cgcagccgga cacgaacacg agccggccgg cccggaccac gtggacgaag
     2821 tggctgatcg gctcgggcat gcccggcacc cagatctctt cgcggtgagc gctcgtggcc
     2881 atcggattct cctccgtcgg gtgagggtgg gggggtcgcg tcgcgcggcg attattctcc
     2941 gcccggcgcg gccggtcaag cggtgaccgc cgagggccga tggtctgaca ccacctgtca
     3001 accgaccgcc ggcgaccccc ggcgaccgcg cggatttacg gtcgatttca cgcggcctgg
     3061 ctggtccgac tcttgctgat cctgaacgcc atgatcagcc gactcgccac cgccgccctc
     3121 gccgcgctcc tgctcctgat ctgctccgcc gcgccgacgc actcgcaggc cctcgacccc
     3181 gcctccgccg acgccctggc gaccacgctc cgcatgctgg ccgaccccgc cacacggggc
     3241 cggatcatcg ccagcgaccc gagcgcggcc gagatcgacc gccaggtgca ggggatggcg
     3301 ggctcctccg cgctcgtcca ggaggtctac aggctcgccg cggaggtatt cgccgacctg
     3361 acccgcaact ccaacggcga cccccgggcc atgagcgcgg cgctcgatcg ggccaagtcc
     3421 gacccggccg gcttcgcggc catgctccgc ccggagacac tggagcggct ccgcgcgctg
     3481 tccatcaaga tctccgacac ccagcgatga cctccagcat gaccaccaga atgaccgagg
     3541 acccgatcag cctgctgaag ggacgcatgg ccgaggccct ggtcgaggcg gtcttcaagc
     3601 gggcaaagta cacggtggcg cgggtcggcc gtgagaccca gatgccggcg ctcgtgcgga
     3661 gtggccgcac cgagttcatg ccggacttca tggtgtggcg ggccctcgat gggccgagcc
     3721 tcggccatca ctcctaccgc ctggtcgcca tcgaggtgaa gtatcgcgcg gacctggcgg
     3781 actacctgtg tcgcgactcg ccctcgcggt cggccgaggt ggcccagcag tggccggagc
     3841 tctacgagat cctcgtgacc gaccggcccg aggtcggccg gtcctgcttc caggcgatcc
     3901 acgtctccga ggtgcccgcg ggcacggtgc cgatcacgct cgacctgcat caggtctcgg
     3961 gcctcgacat ctaccgccgg acggtggagg agtacgaggg gctcgtgcgg gagatctttc
     4021 cgattcttgg atcacgtcag ggctcgggcc aggggtgcgt cgagccggag cggaagcccc
     4081 tggcgcggat gctcggccgg aaccagggcc gcggccaccg gctcacccgg ccggcccgcg
     4141 acccgcaccg ctaccgtccg tcggtggtca gcgagcaccg accgggcgcg agcgagtagc
     4201 gcagccccga cgtc
//
LOCUS       DATMCV010000737         2591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_442715, whole genome shotgun sequence.
ACCESSION   DATMCV010000737 DATMCV010000000
VERSION     DATMCV010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442715"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1621
                     /locus_tag="VNU03_16940"
     CDS             110..1621
                     /locus_tag="VNU03_16940"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HWP76211.1"
                     /translation="MRKLRAKSFKSIPGKYYRTPKELWGFRTDRGDGSPSAIARTFLE
                     TNRDTLGITSALDRLDVRRVIHAQGADHVILQQLWRRRRIHRAYVTVHIGRDRRVYLV
                     KSRAVPEDVLRRAEEPRISSATAVRAAMAGGGRGTGRAWVVGKPELLWYPARRLLHPA
                     WRVRIHRTKPRAEVITYVHAVTGTILSRYDNLSEATGRATVFLPNPMALARGFEPLHG
                     TRLQRPPASAYALVKLTGLRSGIQLNGRRVTTRLTKSRLRSARLDFSVSSARAGFEEV
                     SAYYHVNEAMAYLEQLGYRGPRRLFAKPLEIDARGTRDDNSWYSPGERTLTFGTGDVD
                     DAEDGETVLHEFGHAIQDAICPDFGQSPEAAAMGEGFGDYFAGSFFAAKKPPDYRDAV
                     MTWDGARGDGTPPCVRRLDSALTYESFDHGTTADEHENGQIWSATLWDIWKATGRRVA
                     DRMIVESHFQLDGFTTFARGARAILDADRNLYRKRHATILKRIFRRRGIGPVE"
     gene            complement(1992..2174)
                     /locus_tag="VNU03_16945"
     CDS             complement(1992..2174)
                     /locus_tag="VNU03_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76212.1"
                     /translation="MTAATLFFGVLAAFVAFLEEHRRCGELDGGTDDGRVWLACTCGA
                     EIAHPVVPSPPVHGRP"
     gene            2321..2521
                     /locus_tag="VNU03_16950"
     CDS             2321..2521
                     /locus_tag="VNU03_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76213.1"
                     /translation="MEIIGPIQAVGPDHVVMLTGERIAVPADLLARAKIVVGLFATIS
                     VEEVDGQLVASDIRLHVDRLTL"
BASE COUNT          409 a          934 c          853 g          395 t
ORIGIN      
        1 gacggccagg cgacccgacc ttactcaggc atcccctgcc ttggccaggg ctacttatcg
       61 gctggccatg ctacgattcc tcgaccgaac gcccttcgtg ggggtcctca tgaggaagct
      121 ccgagcgaag tcgttcaaga gcattccggg caagtactac cgcactccga aggagctctg
      181 gggctttcgc accgaccggg gcgatggatc gccgtcggcg atcgcgcgga cctttctcga
      241 gaccaaccgc gacaccctcg gcatcacctc cgcgctcgac cgcctcgacg tgcgccgcgt
      301 gatccacgcc cagggcgccg accacgtcat cctccagcag ctctggcgtc gccgccgcat
      361 ccaccgcgcc tacgtcaccg tgcacatcgg ccgggatcgg cgggtctacc tggtgaagag
      421 ccgcgcggtg ccggaggacg tgctgcgccg ggccgaggag ccccgcatct cgagcgcgac
      481 ggcggtgcgg gccgcgatgg ccggaggagg gcgcggcacc gggcgggcgt gggtggtcgg
      541 caagcccgag ctgctctggt acccggcccg gcggctgctc catcccgcct ggcgcgtgcg
      601 catccaccgc acgaagccgc gcgcggaggt catcacctac gtgcacgcgg tcaccgggac
      661 gatcctcagc cgctacgaca acctctccga ggcgacgggc cgggcgaccg tgttcctgcc
      721 gaaccccatg gccctggccc gcggcttcga gccgctccac gggacgcgac tgcagcgccc
      781 tccggcgtcg gcgtacgccc tcgtgaagct caccggcctt cggagcggga ttcagctgaa
      841 cgggcgacgc gtgaccacgc gtctcacgaa gtcgcgcctg cggagcgcgc gcctcgactt
      901 ctcggtgtcg agcgcccgcg ccggcttcga ggaggtctcg gcctactacc acgtcaacga
      961 ggccatggcg tatctcgagc agctcggcta ccggggcccg cgacgccttt tcgccaagcc
     1021 cctcgagatc gacgcgcggg gcacgcgcga cgacaactcg tggtacagcc cgggcgagcg
     1081 caccctgacc ttcggcaccg gtgacgtgga cgacgcggag gacggcgaga ccgtgctcca
     1141 cgagttcggg cacgccatcc aggacgccat ctgtcccgac ttcggccagt cgcccgaggc
     1201 ggccgcgatg ggcgaaggct tcggcgacta cttcgcgggc agcttcttcg cggcgaagaa
     1261 accgcccgac taccgcgacg cggtgatgac gtgggacggc gcgcgcggcg acggcacgcc
     1321 gccgtgcgtc cggcggctcg actcggccct cacctacgag agcttcgacc acggcacgac
     1381 cgccgacgag cacgagaacg gccagatctg gtcggccacc ctgtgggaca tctggaaggc
     1441 gaccgggcgg cgggtggcgg accgcatgat cgtcgagagc cacttccagc tcgacggctt
     1501 caccaccttc gcgcgcggcg cgcgcgccat cctcgacgcg gaccgcaacc tctaccgcaa
     1561 gcgccacgcg accatcctca agcggatctt ccgccgccgc gggatcgggc cggtggagtg
     1621 aggcggtgcg tcacatctga gtgatcctcg tccacgtccg tattcgagga gcgcctggta
     1681 gtcgtcttcg ctgatcattc gcttctccca atcgcaggcc tgggcgcagc tccgctcgat
     1741 gggtctgaag aaccacatga tcctgatata gaccggcttc cagccatacc cgatgctgat
     1801 gcccaccagc tcctcgacca cgccctcgta gggggtacct tcctctcgca ggatgcggga
     1861 gagggtcacc acgtgaggga ggccttcctt cgcgagaaac gtctcccggt tgcgtcgcca
     1921 gtactcggtc tccgatccca tcggacgcgc ttcggctgaa gctcgcgctc agtggaggct
     1981 cgaggacttc ttcagggacg gccgtggacc gggggcgacg ggaccaccgg gtgcgcgatc
     2041 tctgccccac acgtacaggc cagccagacc cggccatcgt cggtgcctcc atcgagctcg
     2101 ccgcatcgcc ggtgctcttc caggaacgcg acgaaagcag ccagcacgcc gaagaacaag
     2161 gttgctgccg tcatggcgag atcctcccgt ctggccgaat atcgaagcgg gacggggcgg
     2221 agcggggacg accagggaag gaacgtatac cagggcggcg cggggtccgc aagacgttct
     2281 cggggccggg cgtgcccggc ttgtgctatc gtcccgtacc ttggaaatca tcggccccat
     2341 ccaggcggtc ggtccggacc acgtcgtcat gttgacgggc gagcggattg cggtcccggc
     2401 cgatctcctg gcgagagcaa agatcgtcgt cggcctcttc gccacaatct cggtcgagga
     2461 ggtggacggg cagctcgtcg cgtctgacat ccggctgcac gtcgaccggc tcacgctctg
     2521 accgggcgcg cgactcatcc tcgcccgtca cccggtcgct ccatctcact cgatcacctg
     2581 atccgcccgc t
//
LOCUS       DATMCV010000738         4740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_238351, whole genome shotgun sequence.
ACCESSION   DATMCV010000738 DATMCV010000000
VERSION     DATMCV010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238351"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VNU03_16955"
     CDS             complement(<1..527)
                     /locus_tag="VNU03_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HWP76214.1"
                     /translation="MIEGLVGLCAMMVLAFLRVPISYAMGIVGIIGYAYMRDWNWWVA
                     FATAQTKVYETGRNYTLSVVPLFILMGNLVTRAGMSQELFRAAYAFIGHLRGGLAMAT
                     VWASAGFGGICGSSIATAATMAKVAYPSMKRFGYHDQLAAGTVAGAGTLGIMIPPSTI
                     MIIYGVFTETNIGKL"
     gene            complement(524..994)
                     /locus_tag="VNU03_16960"
     CDS             complement(524..994)
                     /locus_tag="VNU03_16960"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HWP76215.1"
                     /translation="MIDRSTWGRRVEGVLGVGSSLILLVMMALTFVDVVARYLFNRPL
                     AGAFEVTELLLLVLIFAGLPLVTYADEHALMDFIDRPLGPRATRVLERLVQLLCAGVM
                     ALLAWLVWLKADRIWAYRDATDVLRIVYGPFVYFMAITIGLTAVIHLYKIVERR"
     gene            complement(991..2013)
                     /locus_tag="VNU03_16965"
     CDS             complement(991..2013)
                     /locus_tag="VNU03_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWP76216.1"
                     /translation="MFTRVVLTALALIGAAAAPALAQTTALTMSSWVSPQHHLTSVVL
                     QGWANEVEKATGGRVKLTMLPKHPSAPPGTFDAVRDGLVDVSYVTASYTPARHILPLM
                     AELPGAGDTALVNSVAYSRIYWKYFHAVGEYKGVKQLGVFTHGPGQMFTKRPVNGIAD
                     VQGLKIRTGGGIAEKVAKALGASAFVKPAPESYELLKGGVADGVFFPFESIISFKLDT
                     VIEQATVFPGGMYSSAFGFFMNEDKWNKLSKEDQAAIEKVSGEHLARLAGRSWDEADA
                     KGLDALKKSGVKIVTADAAFSAEVAKRSAPIVEEWIKSASAKGVDAAKILGEFRAELK
                     KVAAGQ"
     gene            2142..2945
                     /locus_tag="VNU03_16970"
     CDS             2142..2945
                     /locus_tag="VNU03_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76217.1"
                     /translation="MGKRVEGKAAVVVGGGQTPGQTVGNGRATAIVLGREGAHVCVVD
                     RALASAQETVDLIAAEGGRAWAFAADVTREADVERLMAAARERFGRIDILHNNVGASL
                     ALGDAGATDLTEEAFDRSLAVNLKATWLTCKHALPALRETHGSIVNIASMAALNAYPL
                     VGYKTTKAAVVAFTENLAAHNAKYGVRANSILPGLMNTPMAIESRVAAGTPREEVIAA
                     RDRRVPLGRRMGTGWDVANAALFLHSEEAGFITGVSLIVDGGESVAWGS"
     gene            2947..3747
                     /locus_tag="VNU03_16975"
     CDS             2947..3747
                     /locus_tag="VNU03_16975"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWP76218.1"
                     /translation="MAGPPLPENAYRARAERGEIQIGTWITMIRTPSVLTLLQAAGLD
                     YARLDLEHSAFSIETVADMAALARALDFPLVVRPPDGNREWITRLLDAGVWNLHIPQV
                     ESGEHAAAVVAATRYAPLGSRGMYGSGPHTAFRVRPPADHMATANARVHVTAMLESRH
                     AFDRLDEIAAVPGIDALTIGPTDLAQDLGVLGTPAQAAVVREHRIRLGEAARKHGKTV
                     AMAVDTFEAVRDVIALGATIVNYSSDVGVLRSGYAAAVAEIRRPKETA"
     gene            3744..>4740
                     /locus_tag="VNU03_16980"
     CDS             3744..>4740
                     /locus_tag="VNU03_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWP76219.1"
                     /translation="MKIKAVNGFARILKSEGTPWVSCYPTSPVNNALGEEGMPILMMG
                     EERFAVAVADSFSRVTNGKQIGVCTVMAGLNAAGIQMAFGAIAQAWEDSSPLLVIAEG
                     LGVGASRHTHFDMAEAFKPVTKWVGKVDRADLIPEYTRRAYTHLRSGRPGPVLLLIPR
                     DLGEYDADEHPYAPVKGWRTGPDPDDVKSAIRALLSAKDPLLHIGEGVCYADAAAELL
                     QFAEAAQIPVMTTLKAKGVFPENHPLSIGVRGSMVDHFLRKCDLLFSIGSSLFPGRFS
                     HTVPDAHKKTIVQCTVDTLDINRSYETRHAVIGDAKLTLQALADELQAKGARKHPA"
BASE COUNT          744 a         1657 c         1678 g          661 t
ORIGIN      
        1 aagagcttgc cgatgttggt ctcggtgaac acgccgtaga tgatcatgat ggtggacggc
       61 gggatcatga tcccgagcgt ccccgccccc gccaccgtgc ccgccgcgag ctggtcgtga
      121 tagccgaagc gcttcatcga cgggtacgcg accttggcca tggtggccgc ggtggcgatg
      181 gaggagccgc agatgcctcc gaagccggcc gaggcccaga cggtggccat cgcgagcccg
      241 ccgcgcaggt ggccgatgaa ggcataggcg gcccggaaca gctcctgcga catgccggcg
      301 cgggtgacca ggttgcccat caggatgaag agcggcacca ccgacagggt gtagttgcgg
      361 ccggtctcgt agaccttggt ctgggcggtc gcgaaagcga cccaccagtt ccagtcgcgc
      421 atgtaggcgt agccgatgat gccgacgatc cccatcgcgt aggagatggg cacgcgcagg
      481 aaggccagca ccatcatggc gcaaaggccg accagtccct cgatcaccgg cgctcgacga
      541 tcttgtagag gtggatcacc gcggtcagcc cgatggtgat cgccatgaag tagacgaacg
      601 ggccgtagac gatgcgcagc acatcggtgg cgtcccggta ggcccagatc cggtccgcct
      661 tgagccacac cagccaggcg agcagggcca tcacccccgc gcacaggagc tggaccagcc
      721 gctccagcac ccgggtggcg cggggcccga gcgggcggtc gatgaagtcc atcagggcgt
      781 gctcgtcggc gtaggtgacg agcgggaggc ccgcgaagat cagcaccagg agcagcagct
      841 cggtgacctc gaaggcgccc gcgagcggac ggttgaacag gtagcgggcg accacgtcca
      901 cgaacgtcag cgccatcatc accagcagga tcagggagga gccgacgccg aggacaccct
      961 cgacacgccg cccccacgtg gagcggtcga tcactggccg gcggcgacct tcttcagctc
     1021 ggcccggaac tcgccgagga tcttcgccgc gtccacgccc ttggccgacg cgctcttgat
     1081 ccactcctcc acgatcggcg cggagcgttt cgccacctcg gccgagaagg cggcgtccgc
     1141 ggtgacgatc ttgaccccgg acttcttgag cgcgtcgagg cccttggcgt cggcctcgtc
     1201 ccaggagcgg ccggccaggc gcgcgaggtg ctcgcccgag accttctcga tcgcggcctg
     1261 gtcctccttc gagagcttgt tccacttgtc ctcgttcatg aagaagccga acgcggagct
     1321 gtacatgccg cccgggaaga cggtggcctg ctcgatcacg gtgtcgagct tgaacgagat
     1381 gatggactcg aacgggaaga acaccccgtc ggccaccccg cccttgagca gctcgtagga
     1441 ctccggcgcg ggcttcacga aggcggatgc gccgagcgcc ttggcgacct tctccgcgat
     1501 gccgcctccg gtgcggatct tgaggccctg cacgtcggcg atcccgttga ccggccgctt
     1561 ggtgaacatc tggccgggcc cgtgggtgaa caccccgagc tgcttcacgc ccttgtactc
     1621 gcccaccgcg tggaagtact tccagtagat gcgcgagtag gccaccgagt tgaccagcgc
     1681 ggtgtcgccc gcccccggca gctcggccat gagcggcagg atgtgccggg ccggggtgta
     1741 gctggcggtg acgtaggaga cgtcgaccag cccgtcgcgg accgcgtcga acgtgcccgg
     1801 cggggcggac gggtgcttcg gcagcatggt cagcttgacc cgaccgccgg tggccttctc
     1861 cacctcgttg gcccagccct gcagcaccac gctggtgagg tgatgctggg gcgacaccca
     1921 cgacgacatc gtgagcgcgg tggtctgggc gagcgcgggc gccgcggcgg ccccgatcag
     1981 ggccagcgcg gtcagcacga ctctcgtgaa catggccatc ggatggtcct cctcgggcgc
     2041 tacgcgtggg atcggcctgc gggcggtagt gtatcgcagg tcgcgccgtc cgagcgcgat
     2101 ccgcttgcgc agcccgcggg ggcggggtag aagggagcct catgggcaag cgcgtcgagg
     2161 gcaaggccgc cgtcgtcgtc ggaggcgggc agacacccgg ccagacggtg ggcaacggcc
     2221 gggccaccgc catcgtgctg ggccgcgagg gagcccacgt ctgcgtggtc gaccgggcgc
     2281 tcgcttccgc gcaggagacc gtcgatctga tcgcggcgga gggcggccgc gcctgggcct
     2341 tcgcggccga cgtcacgcgc gaggccgacg tcgagcgcct gatggcggcg gcgcgcgagc
     2401 gcttcggccg gatcgacatc ctgcacaaca acgtgggagc gagcctggcc ctcggcgacg
     2461 cgggggccac cgatctgacc gaggaggcct tcgaccggag cctggccgtc aatctgaaag
     2521 caacctggct cacctgcaag cacgcgctgc ctgccctgcg ggagacccac ggctcgatcg
     2581 tgaacatcgc gtcgatggcc gcgctgaacg cctacccgct ggtgggctac aagaccacca
     2641 aggccgcggt ggtcgcgttc accgagaacc tggccgccca caacgcgaag tacggcgtgc
     2701 gcgccaactc gatcctcccc gggctgatga acaccccgat ggcgatcgag tcgcgcgtgg
     2761 ccgcgggcac cccgcgggaa gaggtcatcg ccgcgcgcga ccgccgggtg ccgctcgggc
     2821 ggcgcatggg caccggctgg gacgtggcca acgcggcgct cttcctgcac tccgaggagg
     2881 cgggcttcat caccggggtc tcactcatcg tggacggcgg cgagtccgtc gcctggggct
     2941 cctgacgtgg cggggccccc gctcccggag aacgcgtacc gcgcccgcgc cgagcgcggc
     3001 gagattcaga tcggcacctg gatcaccatg atccgcaccc cgtcggtgct cacgctgctg
     3061 caggcggccg gcctcgacta cgcgcgcctc gatctcgagc actccgcgtt ctcgatcgag
     3121 acggtcgccg acatggcggc cctggcccgc gcgctcgact ttcccctcgt ggtgcggccg
     3181 ccggacggca accgcgagtg gatcacccgg ctgctcgacg cgggggtgtg gaacctgcac
     3241 atcccccagg tggagtcggg cgagcacgcg gccgcggtgg tggcggccac ccgctacgcc
     3301 cccctcggtt cgcgcgggat gtacggctcg ggcccgcaca ccgcgttccg ggtgcgcccg
     3361 cccgccgatc acatggcgac cgccaacgcc cgcgtgcacg tgaccgcgat gctcgagagc
     3421 cggcacgcct tcgaccggct ggacgagatc gcggcggtgc ccggcatcga cgcgctgacc
     3481 atcggtccca ccgacctggc ccaggacctg ggcgtcctcg gcaccccggc ccaggccgcg
     3541 gtcgtgcgcg agcaccggat ccggctgggc gaggcggcgc gcaagcacgg caagacggtg
     3601 gccatggcgg tcgacacctt cgaagcggtg cgggacgtga tcgcgctcgg cgcgacgatc
     3661 gtgaactact cctccgacgt gggcgtgctg cggtcgggct acgcggccgc ggtggcggag
     3721 atccgcaggc caaaggagac ggcatgaaga tcaaggcggt caacggcttc gcgcgcatcc
     3781 tcaagtccga gggcaccccg tgggtgagct gctacccgac gagcccggtc aacaacgcgc
     3841 tcggcgagga gggcatgccc atcctcatga tgggcgagga gcgcttcgcg gtcgcggtgg
     3901 cggacagctt ctcgcgggtg accaacggca agcagatcgg ggtgtgcacg gtcatggccg
     3961 gcctcaacgc ggcgggcatc cagatggcct tcggcgccat cgcgcaggcg tgggaggatt
     4021 cctccccgct gctggtgatc gcggagggcc tcggcgtcgg ggccagccgc cacacccact
     4081 tcgacatggc ggaggccttc aagccggtca ccaagtgggt gggcaaggtg gaccgcgccg
     4141 acctcatccc cgagtacacg cgccgggcct acacgcacct gcgctccggc cggcccggcc
     4201 cggtgctgct gctgatcccg cgcgatctgg gcgagtacga cgcggacgag catccctacg
     4261 cgccggtgaa gggctggcgc accgggcccg atccggacga cgtcaagtcc gcgatccgtg
     4321 cgctgctctc ggccaaggat cccctgctgc acatcggcga gggcgtctgc tacgcggacg
     4381 cggcggccga gctgctgcag ttcgcggagg cggcccagat cccggtgatg accacgctca
     4441 aggccaaggg cgtgttcccg gagaaccacc cgctgtccat cggcgtgcgc ggctcgatgg
     4501 tcgatcactt cctgcgcaag tgcgacctgc tcttctctat cggctcgagc ctcttccccg
     4561 gccgcttcag ccacacggtg ccggacgcgc acaagaagac catcgtgcag tgcacggtcg
     4621 atacgctcga catcaaccgc agctacgaga cgcggcacgc ggtgatcggc gacgccaagc
     4681 tcacgctcca ggcgctggcg gacgagctcc aggccaaggg cgcgcgcaag caccccgcgg
//
LOCUS       DATMCV010000739         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_975751, whole genome shotgun sequence.
ACCESSION   DATMCV010000739 DATMCV010000000
VERSION     DATMCV010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975751"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VNU03_16985"
     CDS             complement(<1..1093)
                     /locus_tag="VNU03_16985"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWP76220.1"
                     /translation="MPPSKSTGAAGRRAGPEAAHPLATALQFVKGIGPQRAKLLANLG
                     LHTVEDALYYLPTRHEDRSQLTPLRSLKPNDVTTVSGTIRAVSPPPRGRPRVPLSALL
                     SDGTGFLTCVWFGQAYLERVFQRGQRLIVHGKVQRYRSGPLQMQVKDFEIVEDESGDG
                     AGEPGDETLHTGRLVPVYGLTRGLTARPMRRLMKRLVDGYVDGLDDPLPDALRARHHL
                     DLLSLALRAGHFPKTDADYASARRRLVYDEFLLLQLGLAIRRHRQGRQPGLAMNPPGA
                     LARRLLASLPFALTPAQERVWREIRMDMAAPYPMNRLLQGDVGSGKTVVAALAILTAV
                     ESGYQTALMAPTEILAEQHLMTLTQLLEPL"
     gene            1176..1367
                     /gene="rpmB"
                     /locus_tag="VNU03_16990"
     CDS             1176..1367
                     /gene="rpmB"
                     /locus_tag="VNU03_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWP76221.1"
                     /translation="MAQRCDVCGKGPAVGNRISHAHNVTKRRWNVNLVSMRARVKGRV
                     ERMKVCTRCLKAGKVVKVV"
     gene            complement(1682..>2133)
                     /locus_tag="VNU03_16995"
     CDS             complement(1682..>2133)
                     /locus_tag="VNU03_16995"
                     /inference="COORDINATES: protein motif:HMM:NF021469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HWP76222.1"
                     /translation="LARPGDLLDAWVEAYSYRQSDVQAYFSPERITRGLVAELGRAAH
                     EARRRYAFTLHSGAALVAPNVRFPAIHCYLEGDPEPVAKALGLRPGEGEGNVYLLAPY
                     DPGVFYAPMAKGGLPVVFLPQLYADLYHYERRGREQAAHLRREAMGY"
BASE COUNT          316 a          734 c          745 g          338 t
ORIGIN      
        1 cgaggggctc gaggagctgg gtcagggtca tcaggtgctg ctcggccagg atctcggtcg
       61 gagccatcag cgcggtctga tagcccgact ccaccgcagt gaggatcgcg agcgcggcca
      121 ccaccgtctt gcccgagccc acgtcgccct gcaggagccg gttcatcgga tagggcgcgg
      181 ccatgtccat ccggatctcg cgccacaccc gctcctgcgc gggcgtgagc gcgaacggaa
      241 gcgaggcgag gagacgccgg gccagggcgc cgggcgggtt catggccagg cccggctgcc
      301 gcccctggcg gtgtcgccgg atcgcgagac cgagctgcag cagcaggaac tcgtcgtaga
      361 cgagccgccg ccgcgcggag gcgtagtcgg catccgtctt ggggaagtgg ccggcgcgca
      421 gggccaggct cagcagatcc aggtgatggc gcgcgcgcag ggcgtccggc agcgggtcgt
      481 caaggccgtc cacgtagccg tcgaccaggc gcttcatgag ccgacgcatc ggccgcgcgg
      541 tgaggccccg ggtcagcccg tagaccggca cgaggcgtcc ggtgtgcagg gtctcgtcgc
      601 cgggctcgcc cgcgccgtcc ccgctctcgt cctccacgat ctcgaagtcc ttgacttgca
      661 tctgcagcgg ccccgagcga tagcgctgga ccttgccgtg gacgatgagc cgctggcccc
      721 gctggaacac gcgctcgagg tacgcctggc cgaaccacac gcaggtgagg aagccggtgc
      781 cgtcgctcag cagcgccgac agcggcaccc ggggccgccc gcgcgggggc gggctgaccg
      841 cgcggatcgt cccggacacc gtggtcacgt cgttgggctt gaggctccgg agcggcgtga
      901 gctggctgcg gtcctcgtgg cgcgtcggga ggtagtagag ggcgtcctcc acggtgtgga
      961 ggcccaggtt ggcgaggagc ttggcccgct gcggcccgat gcccttgacg aactgcagcg
     1021 ccgtcgcgag gggatgggcc gcctccgggc ccgcgcggcg gcccgcggcg cccgtcgact
     1081 tactcggggg caacggtttg cctcatgcca gtcgccatgc tataaagaaa cgcttcgtcc
     1141 ggcaaaccac acacacaact cagcagggga tcagcatggc tcagcgttgc gatgtctgcg
     1201 ggaagggtcc ggccgtcggc aaccgcatca gtcacgctca caacgtgacc aagcggcgct
     1261 ggaacgtcaa cctcgtgtcg atgcgggcac gggtcaaggg cagggtcgag cgcatgaagg
     1321 tctgcacgcg ctgtctcaaa gcgggcaagg tcgtcaaggt cgtctgaggt tcgagcctga
     1381 aaagggggcg gggggtgtca ccaccccccg ccgtccttct gaagccttgc agtagctagt
     1441 aagagtcccg gtgggaggcc gccacccccc accgcacgac aacagcgtta cttcttcttc
     1501 ttcttggccg ccttcttctt cttcgccatg aggtcgtcac cccctttcct gagaagactc
     1561 acaactcgcg cgcggctgac tacttccgct tacccttctt ggtggccttc ttcttgccac
     1621 ccttcttgac ggccttcttg cgcttcggca tgtggggtct cacctccctc ccctctggga
     1681 ttcagtagcc catcgcttcg cgccggaggt gtgcggcctg ctcccgcccc cgtcgctcgt
     1741 agtggtagag atcggcgtag agttgtggca ggaagaccac cgggagcccg cccttcgcca
     1801 tcggggcgta gaagacgccc ggatcgtagg gggccagcag gtagacgttg ccctcgcctt
     1861 cccccgggcg cagcccgagg gccttcgcca ccggctcggg atcaccctcg aggtagcagt
     1921 ggatggccgg gaaccgcacg ttgggagcca ccagcgccgc gccggagtgc agcgtgaacg
     1981 cgtagcgccg gcgtgcctcg tgagccgccc gcccgagctc ggccaccagc ccccgggtga
     2041 ttcgctccgg tgagaagtag gcctggacgt cgctctgccg gtaggagtag gcctccaccc
     2101 aggcgtccag caggtcgccc ggccgggcca gct
//
LOCUS       DATMCV010000740         2826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_106511, whole genome shotgun sequence.
ACCESSION   DATMCV010000740 DATMCV010000000
VERSION     DATMCV010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106511"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(712..1647)
                     /locus_tag="VNU03_17000"
     CDS             complement(712..1647)
                     /locus_tag="VNU03_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP76223.1"
                     /translation="MSDNQAVVVDPSAPGRLVLRPVPAPTPDRGEALIRVRAISLNRG
                     EVRRSTMAPAGWRPGWDLAGTVERVASDGSGPKAGARVVGFLPEGAWAERVAVPTHAV
                     AELPEKVTFSQAATFPVAGLTALHALGKNGMILGKRVLVTGATGGVGDFAVQLARLGG
                     AHVTAAVRRADQAAAVRQLGAHEVTVGDDIPATPKYDLVIESVGGRTLGTALAALERG
                     GVCVTLGVSASADVTFDARAFFATGRTQLYGFYIFTELGNEPAAIGLRRLADLVAAGQ
                     LAPHISVERPWTDIAQVAADLIARKYPGKAVLTLA"
     gene            complement(1699..>2826)
                     /locus_tag="VNU03_17005"
     CDS             complement(1699..>2826)
                     /locus_tag="VNU03_17005"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP76224.1"
                     /translation="QMLAWLGADVIKIEPPTGEPGRTALSDKRGEDAWFFLLLNSGKK
                     GVTLNLKSERGRSMFLEMIKSADVLVENMGPGAMDRLGLGWDTLQRLNPRLIAASVKG
                     FGGGGPYADYKSFEWIAQAMAGAMSMTGSPDGPPTKAIGGLADTGAGLHTAIGILAAI
                     IQRQATGKGQQVEVAQQEAVVNLLRIHLRESYATGKPAPRQGNRSAAAAPSNIYRCSP
                     GGPNDYVFIHCATVDMWKTLTRIVGRPEMADDPRYHDRRDRVQIVDEIDAMIEAWTET
                     RNKHEVMEIMAGAGVPCGAVLDSTEVLSDPHMRKRRFVTDLEHPRRGPYPMPGNPVRL
                     SDSPTEVTRSPLLGEHNTEVYSRLLGYKPEAIDELKRDGVI"
BASE COUNT          398 a          930 c         1044 g          454 t
ORIGIN      
        1 actcgattcc cagcaatgaa gctcgcagat gaatatctag ctctgatagc gtccggagtg
       61 tcccaatcgg ctgtttctgc ttctgccagc caactgtcga gaggtccccg cgcatccgcg
      121 tgacttttct tgaaatcctc caatgagccc cggccgacaa cgatcacctc tcgacagggc
      181 taccgaactg gctagcgtta ccctttccgt tcccgtaggg ggaatatagt tatagttccc
      241 acacagggaa cattctagta gtacgccagg gcttgtcaag aggatttttc aattagggac
      301 ccgatttcgc cgttgccgca gatattgagc aacccagcca atttgtgcag gagaagggga
      361 gccacgtttc cgcggaaact ccgggggaag ctggaagggg ggaaccgcat ggcggtgtgc
      421 cacccggtcg gttcctcgtg cagcagccgg gggcggggac ccggacgatc cccccttgcg
      481 gtgggagctc cgctgcccga ctgccatccg cctgccgtgg tgcccgacgc tgccgtcgag
      541 acgctacatg cgcccggccc ggctcagacg attccacgcg cgcacggcct gccatggggg
      601 ctcgggacca cgtggatgcg gatcgggcga tcgatgtcct acatccccgg ggcgcgaccc
      661 ctcccgcgcg tttccgcgga aacgtcctag cgcgagccgc gcggcatcag ctcaggcgag
      721 cgtcagcacc gccttgcccg gatacttgcg cgcgatcaag tccgccgcca cctgcgcgat
      781 gtcggtccac ggccgctcca cgctgatgtg gggggcgagc tggcccgcgg ccaccaggtc
      841 cgccaggcgg cgcaggccga tcgcggcggg ctcgttgccc agctcggtga agatatagaa
      901 cccgtagagc tgggtgcggc cagtggcgaa gaaggcgcgg gcgtcgaagg tcacgtcggc
      961 tgaggcggag acgccgagcg tgacgcagac gccgccccgc tcgagcgcgg ccagcgcggt
     1021 gccgagggtg cggccgccga ccgactcgat caccaggtcg tacttgggcg tggcggggat
     1081 gtcgtcgccg acggtgacct cgtgggcgcc gagctggcgg acggcggcgg cctgatcggc
     1141 gcggcggacg gcggcggtga cgtgggcgcc gcccaggcgc gcgagctgca ccgcgaagtc
     1201 gccgacgcca ccggtggcgc cggtcaccag cacgcgcttg ccgaggatca tgccgttctt
     1261 gccgagggcg tggagcgcgg tgaggccggc gaccgggaag gtcgcggcct gggagaacgt
     1321 caccttctcg ggcagctcgg ccactgcgtg ggtcggaacc gcgacgcgct cggcccaggc
     1381 gccttccggc aggaagccca ccacgcgggc gccggccttg gggccggagc cgtcggaggc
     1441 gacgcgctcc acggtgccgg ccaggtccca gcccgggcgc cagcccgcgg gggccatcgt
     1501 cgagcggcgg acctcgccgc ggttcagcga gatggcgcgg acgcggatga gcgcttcgcc
     1561 gcggtcggga gtgggggcgg ggaccgggcg gaggacgagg cggcctggag cgctagggtc
     1621 tacgaccacg gcttggttgt cgctcacttg cattctcccc tcaccctgcc ctctccccag
     1681 aggggagagg gttaggggtt agatgacgcc gtcgcgcttg agctcgtcga tggcctcggg
     1741 cttgtagccg aggaggcggc tgtagacctc ggtgttgtgc tcgcccagga gcggcgaacg
     1801 cgtcacctcg gtgggcgagt cggagaggcg caccgggttg ccgggcatcg ggtaggggcc
     1861 gcggcgcggg tgctcgaggt cggtgacgaa gcggcgcttg cgcatgtgcg ggtcggacag
     1921 cacctcggtg gagtcgagca ccgcgccgca ggggacgccg gcgccggcca tgatctccat
     1981 gacctcgtgc ttgttccgcg tctcggtcca cgcctcgatc atcgcgtcga tctcgtcgac
     2041 gatctgtacg cggtcgcgcc ggtcgtggta gcgcgggtcg tcggccatct cggggcggcc
     2101 gacgatgcgg gtgagcgtct tccacatgtc gacggtggcg cagtggatga acacgtagtc
     2161 gttgggcccg ccggggctgc agcggtagat gttggacggc gcggcggccg cggagcggtt
     2221 gccctgccgc ggggccggct tgccggtggc gtagctctcg cgcaggtgga tgcggagcag
     2281 gttgaccacc gcctcctgct gggcgacctc gacttgctgg cccttgccgg tggcctggcg
     2341 ttgaatgatt gcggccagga tgccgatcgc ggtgtggagc ccggcgccgg tgtcggcgag
     2401 cccgccgatg gccttggtgg gcggcccgtc gggcgagccg gtcatgctca tcgcgccggc
     2461 catggcctgc gcgatccact cgaagctctt gtagtccgcg tagggcccgc ccccgccgaa
     2521 gcccttcacg gaggccgcga tcagccgcgg gttcagccgc tggagggtgt cccagccgag
     2581 tcccaggcga tccatcgcgc cggggcccat gttttccacc aggacgtccg cgctcttgat
     2641 catctcgagg aacatggagc ggccgcgctc cgacttgagg ttgagcgtga cgcccttctt
     2701 gcccgagttc agcagcagga agaaccacgc gtcctcgccg cgcttgtcgg agagcgcggt
     2761 gcggccgggc tcgccggtgg gcggctcgat cttgatgacg tccgcgccga gccacgccag
     2821 catctg
//
LOCUS       DATMCV010000741         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_574008, whole genome shotgun sequence.
ACCESSION   DATMCV010000741 DATMCV010000000
VERSION     DATMCV010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574008"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VNU03_17010"
     CDS             complement(<1..323)
                     /locus_tag="VNU03_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HWP76225.1"
                     /translation="MVSGPYRTGAADAEQRTRNLAVLNEAALALFRAGHVPIVGVNVA
                     LPLIEAAGATTFDEIMMPLSLALVDRCDACLRVGGASTGADQEAQRFRATGRPVYGSV
                     AEVPGT"
     gene            complement(349..1758)
                     /locus_tag="VNU03_17015"
     CDS             complement(349..1758)
                     /locus_tag="VNU03_17015"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP76226.1"
                     /translation="MNATDLAYTPATDLIARIRSKALSPVEVTRAVLERIEQANPKIN
                     AFVTVTGDAALAAARAAEAAVMKGERIGPLHGIPVSIKDLALVKGVPAKFGSPVFEQR
                     IGEVDAPFVRRLREAGAILTGKTSTPEFGWKALGDSPLTGITRNPWNTAMTSGGSSAG
                     AGAAAAAGLGPLHQGSDGAGSVRVPAAFCGIYGLKPTFGRVPMWPVSNTDSASHVGPM
                     TRTVVDAALMLAVMAGPDDWDRQSLDAPPADYVGRLRDGVKGLRVGWSLDLGCCRVDP
                     EIGQVARRAAEAFQELGCTVEDAKVSLPDTREMIHLMWNAHYAGNYAPFLEKFRARMD
                     PGLVAALEDGRRYTAEAYVEMRARKHTYCDAVRELFDTYDLLLTPTVSVAAFEVGRLN
                     PAHFPQHAWDWFPWAGFSYPFNFTGQPAATVPAGFTASGMPVGLQIVGRRLADLQVLQ
                     ASAAFEEARPWAAKRPALG"
     gene            complement(1808..>2542)
                     /locus_tag="VNU03_17020"
     CDS             complement(1808..>2542)
                     /locus_tag="VNU03_17020"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWP76227.1"
                     /translation="LGAGFLVMERATGRPLLDDRVLGVSATLASAQARLHALDAEPVL
                     RALDDEGRAAGGGFDRSVVGYEGYLAALDRRIRRAELTGLAPGVRWLRDRGPAAGPRV
                     ICHGDFHPQNLLAERGRVTAVLDWPNTLVAEPECDVAATLVILRLTPIEVLPVPAALR
                     PLVALLRPLMTARYLARYRRARALDPGRLAYYEAGACMRGLVRAAEARRSGDTNPLDA
                     SSFGERLAARFARLTGVPVALPPSRG"
BASE COUNT          348 a          926 c          960 g          308 t
ORIGIN      
        1 gtccccggca cctccgcgac gctgccgtag accgggcggc ccgtcgcgcg gaaccgctgg
       61 gcctcctggt ccgctccggt ggatgcgccg cccacgcgca ggcacgcgtc gcagcggtcc
      121 accagggcga gcgagagcgg catcatgatc tcatcgaagg tcgtcgcacc ggcggcctcg
      181 atcagcggga gcgccacgtt gacgccgacg atcggcacgt gtccggcgcg gaagagggcc
      241 agcgcggcct cgttcagcac ggccaggttt cgcgtgcgct gctcggcatc ggccgcgccg
      301 gtgcggtagg gtcccgacac catgatccac atatgatcgg gccgcggctc agccgagcgc
      361 cgggcgcttc gccgcccacg gccgtgcctc ctcgaatgcc gcggaggcct gcagcacctg
      421 gagatcggcg aggcgccggc ccacgatctg caaccccacc ggcatgccag acgcggtgaa
      481 gcccgcgggc accgtcgcgg cgggctggcc ggtgaagttg aacgggtagc tgaagcccgc
      541 ccacggaaac cagtcccacg cgtgctgcgg gaagtgggcg gggttcaggc gcccgacctc
      601 gaaggcggcc accgacacgg tgggcgtgag cagcaggtcg taggtgtcga acagctcgcg
      661 caccgcgtcg cagtaggtgt gcttgcgcgc gcgcatctcc acgtaggcct cggcggtgta
      721 gcgccggccg tcctcgaggg ccgcgaccag gcccgggtcc atccgggcgc ggaacttctc
      781 gaggaagggg gcgtagttgc ctgcgtagtg cgcgttccac atcaggtgga tcatctcgcg
      841 cgtgtcgggc aggctcacct tggcatcctc gacggtgcag cccagctcct ggaaggcctc
      901 cgccgcgcgc cgggccacct ggccgatctc gggatcgacg cggcagcagc cgaggtcgag
      961 gctccacccg acccgcagcc ccttgacgcc gtcgcgcagg cggcccacgt agtccgcggg
     1021 cggcgcgtcg agcgactggc ggtcccagtc gtcgggcccg gccatcaccg ccagcatgag
     1081 cgcggcgtcg acgacggtgc gggtcatcgg acccacgtgc gaggcgctgt cggtgttgga
     1141 gaccggccac atcggcacgc ggccgaaggt gggcttgagg ccgtagatgc cgcagaaggc
     1201 cgccggcacc cgcaccgagc ccgccccgtc cgagccctgg tggagcggcc ccaaccccgc
     1261 ggcggccgcg gcgcccgcgc ccgcgctcga gccgcccgag gtcatcgcgg tgttccacgg
     1321 gttgcgggtg atgccggtga gcgggctgtc gccgagcgcc ttccagccga actcgggggt
     1381 gctcgtcttg ccggtcagga tcgcccccgc ctcgcgcagg cggcgcacaa acggggcgtc
     1441 gacctccccg atgcgctgct cgaagacggg ggagccgaac ttggccggca cgcccttgac
     1501 gagcgccaga tccttgatcg agaccgggat gccgtggagc gggccgatcc gctcgccctt
     1561 catgaccgcc gcctccgcgg cgcgcgcggc ggccagtgcg gcgtccccgg tgacggtcac
     1621 gaacgcgttg atcttcgggt tcgcctgctc gatgcgctcc agaaccgcac gcgtcacctc
     1681 caccggggag agggccttgg agcggatgcg ggcgatgaga tcggtggcgg gagtgtaggc
     1741 gagatcggtc gcgttcatgg gcctctccgg agcgaaggga tcggaatccg cgggaatggt
     1801 agcacggtca gccgcgcgac ggcggcagcg ccaccggcac gccggtcagc cgcgcgaacc
     1861 gcgcggcgag gcgctcgccg aaggacgaag cgtcgagcgg attcgtgtcg ccggaccgcc
     1921 gcgcctccgc ggcgcgcacc aggccccgca tgcacgcgcc ggcctcgtag taggcgaggc
     1981 gccccgggtc gagggcccgg gcgcgccggt agcgcgcgag atagcgcgcg gtcatgagcg
     2041 ggcgcagcag cgccaccagc gggcgcagcg cggccggcac cggcaggacc tcgatgggcg
     2101 tgaggcgcag gatgacgagc gtggccgcca cgtcgcactc cggctcggcg accagcgtgt
     2161 tcggccagtc gagcacggcg gtcacccgcc cgcgctctgc gaggaggttc tggggatgga
     2221 agtcgccgtg gcagatcacg cgcggcccgg cggccggccc gcggtcccgc agccagcgca
     2281 cgccgggcgc gaggccggtc agctcggccc ggcggatgcg ccggtcgagc gcggcgagat
     2341 aaccctcata gccgaccacg ctccggtcga agccgccgcc ggccgcccgg ccctcgtcgt
     2401 ccagcgcgcg cagcaccggc tctgcgtcga gggcgtgcag gcgcgcctgg gcactggcca
     2461 gcgtcgcgct caccccgagg acccggtcgt cgagcagcgg ccggccggtg gcgcgctcca
     2521 tcacgaggaa gccggcgccg ag
//
LOCUS       DATMCV010000742         3780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_106673, whole genome shotgun sequence.
ACCESSION   DATMCV010000742 DATMCV010000000
VERSION     DATMCV010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106673"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VNU03_17025"
     CDS             complement(<1..707)
                     /locus_tag="VNU03_17025"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWP76228.1"
                     /translation="MPKTVKRAVLAYSGGLDTSVILRWLIETYRCEVVAYCADLGQGE
                     ELIPVKEKALRTGASTVHIVDLREEFVRDFVFPMLRANAVYEGSYLLGTSIARPLIAK
                     AQVDVARKESADAVSHGATGKGNDQVRFELTYAALAPDLTVIAPWREWDLNSRTALME
                     FARRHDIPVPVTAERPYSTDRNLFHISFEGGILEDPWNEPPPKMFLLTNSPESAPDVP
                     VYVELDYEAGNPVAVDGR"
     gene            complement(725..1636)
                     /gene="argF"
                     /locus_tag="VNU03_17030"
     CDS             complement(725..1636)
                     /gene="argF"
                     /locus_tag="VNU03_17030"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWP76229.1"
                     /translation="MKHFLSIRDLAREDLPRLFALMAELKAKTKARDRSTPLPGRTMA
                     LIFEKPSLRTRVTFEVAMIQLGGAAVYLAAQEIGMGKRESVPDVARNLSRWVDVVAAR
                     VFSHRTLVQLAANSSVPVINGLSDLEHPCQAMADFFTLWERGVDLAKLRLAWVGDGNN
                     VCNSLLLVGAMLGTSAVVATPPGYEPDAAVLEACRAMGGRVRVTTEAREALEDADVVY
                     TDTWISMGQESDREQRLEAFQRYQLNDRLLGFAPGNALVMHCLPAHRGEEITDAVLDG
                     PRSLILEQAENRLHAQKAIILNLLGGD"
     gene            complement(1633..2835)
                     /locus_tag="VNU03_17035"
     CDS             complement(1633..2835)
                     /locus_tag="VNU03_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWP76230.1"
                     /translation="MTDTKTLIEWADKHLMYTAKRAPVALVRGEGVRVWDSDGKEYLD
                     FTGGIAVTALGHSHPKVVGTMREQAATLLHVSNLFYIPQQTQLAKLLCDHSFADRVFF
                     SNSGAEANESAIKLARKWAKEHGASDRGDIITMRGGFHGRTLATVTATAQEKYHHGFE
                     PLPGGFKYVPFNDLRAVERAIDSHTAAVMVEPIQGEGGINIPDDGYLPGLRKLCDEAG
                     ILLVLDEIQTGMGRTGRLWGYEHSGIEPDIMTLAKALANGVPIGATLATESVASAFTP
                     GTHGSTFGGNPFATAVGVTVFATLMEERLPERAAAMGRVLREHLEALRARHPKAATAV
                     RGRGLLVGMDLVPPVGEVISACRERGLLALSAGDNTLRLAPPLVVDEAAIRHAVEIID
                     QALAGFAK"
     gene            complement(2864..>3780)
                     /gene="argB"
                     /locus_tag="VNU03_17040"
     CDS             complement(2864..>3780)
                     /gene="argB"
                     /locus_tag="VNU03_17040"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWP76231.1"
                     /translation="GSFPLHLIGTDERTMAIPEMVQRAEVLIEALPYIRAFQGKTLVI
                     KYGGAAMEQADLKEQFAKDVLLLRLVGIRPVIVHGGGPQIGALMKRLGKEPRFVGGMR
                     VTDEETVQIVEMVLVGKINKEIVGLINLHGGRAVGLSGKDASLLRARKRLHRMPDGTS
                     VDIGLVGEVEAVNPEPIRLLEENGFIPVIAPVGVGESGETYNINADLVAGDVAAALSA
                     EKLIHLTDVTGINGEDGRLVSTLTKRDAERLIKAGVIDGGMLPKVESSLRALTGGAQK
                     AHIIDGRVPHAILLEVLTKEGIGTEIVL"
BASE COUNT          539 a         1326 c         1310 g          605 t
ORIGIN      
        1 cggccgtcca ccgccaccgg gttgcccgcc tcgtagtcga gctcgacgta caccggcacg
       61 tccggcgccg actccggcga gttggtgagc aggaacatct tgggcggggg ctcgttccac
      121 ggatcctcga ggatgccgcc ctcgaacgag atgtggaaca ggttgcggtc cgtcgagtac
      181 ggccgctccg cggtcaccgg caccgggatg tcgtggcggc gggcgaactc catgagcgcg
      241 gtgcgggagt tgagatccca ctcccgccac ggcgcgatca cggtcaggtc gggggccagc
      301 gcggcgtagg tcagctcgaa gcgcacctgg tcgttgccct tgccggtggc cccgtggctc
      361 accgcgtccg ccgattcctt tcgcgccacg tccacctgag ccttggcgat gaggggccgc
      421 gcgatcgagg tgcccaagag gtaggagccc tcgtagaccg cgttggcgcg gagcatcggg
      481 aagacgaagt cgcggacgaa ttcttctcga aggtcgacga tatgcacggt ggacgcgccg
      541 gtgcggagcg ccttctcctt gaccgggatc agctcctcgc cctgccccag gtccgcgcag
      601 tacgccacga cctcgcagcg gtaggtctcg atgagccagc gcaggatgac cgaggtgtcg
      661 aggcctcccg agtacgcgag cacggcccgc ttgaccgtct tgggcatagc cgtcctggac
      721 acgatcagtc cccgccgagc aggttgagga tgatggcctt ctgggcgtgc agccggttct
      781 ccgcctgctc caggatcagg ctgcgcggcc cgtcgagcac cgcgtcggtg atctcctccc
      841 cgcggtgggc gggcaggcag tgcatgacca gcgcgttccc gggggcgaag ccgaggagcc
      901 ggtcgttgag ctggtagcgc tggaacgcct cgagccgctg ctcgcgatcg ctctcctggc
      961 ccatgctgat ccaggtgtcg gtgtagacga cgtcggcgtc ctcgagcgcc tcgcgcgcct
     1021 cggtggtgac gcgcacgcgc ccccccatcg cccggcaggc ctccagcacc gccgcgtccg
     1081 gctcgtagcc cggcggggtg gccaccaccg cgctggtgcc cagcatcgcc cccaccagca
     1141 ggagcgagtt gcagacgttg ttgccgtcgc ccacccacgc gagccgcagc ttggccaggt
     1201 cgacgccccg ctcccagagc gtgaagaagt cggccatcgc ctggcaggga tgctcgagat
     1261 cggagagccc gttgatcacc gggaccgacg agttcgcggc gagctgcacg agggtccggt
     1321 gggagaacac gcgcgccgcc accacgtcca cccagcggga gaggttgcgg gccacgtcgg
     1381 gcaccgactc ccgcttgccc atgccgatct cctgggcggc caggtacacc gcggcgcctc
     1441 ccagctggat catcgcgacc tcgaaggtca cgcgcgtgcg cagcgacggc ttctcgaaga
     1501 tcagggccat ggtgcgcccg ggcagcgggg tcgagcggtc gcgcgccttg gtcttcgcct
     1561 tgagctcggc catgagcgcg aagaggcgcg ggaggtcctc ccgggccagg tcgcggatcg
     1621 acaggaagtg cttcacttcg cgaagccggc cagggcctgg tcgatgatct cgaccgcgtg
     1681 acggatggcg gcctcgtcca ccaccagggg tggggcgagg cggagcgtgt tgtcgcccgc
     1741 gctcagcgcg agcaggccgc gctcgcgaca ggccgagatc acctcgccaa ccggcggcac
     1801 caggtccatg ccgaccagga ggccgcggcc ccgcaccgcg gtcgccgcct tcggatggcg
     1861 cgcgcgcagc gcctccaggt gctcgcgcag cacgcggccc atggcggccg cccgctccgg
     1921 cagccgctcc tccatcaggg tggcgaagac ggtcacgccg accgcggtgg cgaacgggtt
     1981 gccgccgaag gtcgagccgt gggtgccggg cgtgaacgcg gacgccacgc tctcggtggc
     2041 gagggtggcc ccgatcggca ccccgttggc gagagccttg gccagggtca tgatgtccgg
     2101 ctcgatcccg gagtgctcgt agccccacag ccggccggta cgacccattc cggtctggat
     2161 ctcgtccagc accaggagga tgccggcctc gtcgcagagc ttgcggagac cggggagata
     2221 gccgtcgtcc gggatgttga tcccgccctc gccctggatc ggctcgacca tcaccgcggc
     2281 ggtgtggctg tcgatggccc gctccaccgc gcgcaggtcg ttgaacggta cgtacttgaa
     2341 cccgccgggc agcggctcga agccgtggtg gtacttctcc tgggcggtgg cggtcacggt
     2401 ggcgagcgtg cgcccgtgga agccgccccg catcgtgatg atgtcgccgc ggtcgctggc
     2461 cccgtgctcc ttggcccact tgcgcgcgag cttgatcgcg gattcgttgg cctccgcgcc
     2521 cgagttcgag aagaagaccc ggtccgcgaa cgagtgatcg cagagcagct tggcgagctg
     2581 ggtctgctgg gggatgtaga agaggttgga gacgtgcagc agggtcgcgg cctgctcgcg
     2641 catggtcccc accaccttgg gatggctgtg accgagcgcg gtcaccgcga tgccgcccgt
     2701 gaagtcgagg tactccttgc cgtcgctgtc ccacacccgg acgccctcgc cgcgcaccag
     2761 ggccaccggg gcgcgcttgg cggtgtacat caggtgcttg tccgcccact cgatcagcgt
     2821 cttcgtatcc gtcatgtgct tgggcctcgc ggatgaatgc gggtcacaga acgatttccg
     2881 tgccgatacc ttccttggtc agcacttcga gcaaaatggc gtgggggacg cgaccgtcga
     2941 tgatgtgggc cttctgggcc ccgccggtga gcgcccgcag ggaggactcc accttgggga
     3001 gcatgccccc gtcgatgact cccgccttga tcagccgctc cgcgtcccgc ttggtcaagg
     3061 tcgagaccag ccgcccgtcc tcgccgttga tgccggtcac gtcggtcagg tggatgagct
     3121 tctcggcgga cagcgcggcg gccacgtcgc ccgccaccag gtccgcgttg atgttgtagg
     3181 tctcgccgct ctcccccacc ccgaccggag cgatcaccgg gatgaacccg ttctcctcga
     3241 ggaggcggat cggctccggg ttcaccgcct ccacctcgcc gaccaggccg atgtcgaccg
     3301 aggtcccgtc cggcatccgg tgcaggcgct tgcgggcccg caggagactc gcgtccttgc
     3361 cggagaggcc caccgcgcgg cccccgtgca ggttgatcag gcccacgatc tccttgttga
     3421 tcttgccgac cagcaccatc tcgacgatct ggaccgtctc ctcgtcggtg acccgcatgc
     3481 cgcccacgaa gcggggctcc ttgccgagcc gcttcatcag ggccccgatc tgcggccccc
     3541 cgccgtgcac gatgaccggg cggatgccga ccagccggag caggagcacg tccttggcga
     3601 actgctcctt gaggtcggcc tgctccatcg ccgcgccccc gtacttgatc acgagggtct
     3661 tgccctggaa ggcgcgaatg tagggcagcg cctcgatcag aacctcggcg cgctggacca
     3721 tctcggggat cgccatggtg cgctcgtccg tccctataag atgtagcggg aaagatcctc
//
LOCUS       DATMCV010000743         3159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_37426, whole genome shotgun sequence.
ACCESSION   DATMCV010000743 DATMCV010000000
VERSION     DATMCV010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37426"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VNU03_17045"
     CDS             complement(<1..283)
                     /locus_tag="VNU03_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HWP76232.1"
                     /translation="MSQPAPASGARLAVLLGGLGLLVIAAALGALFVGSARVPPGDVL
                     NVLTGRIKGVDQVVVLSLRLPRILAALLAGGALAVAGAGFQALARNPLAE"
     gene            complement(280..1173)
                     /locus_tag="VNU03_17050"
     CDS             complement(280..1173)
                     /locus_tag="VNU03_17050"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HWP76233.1"
                     /translation="MSRFLTALALALTLLPAPAGALTVTDQTGRRVVLPAPPGRIISL
                     VPSVTEILFSIGAQDRLVGVTDFCDYPAEARRKPRVGGMLAPSLEGMVSLKPDLVVAT
                     IAGNRQETFEQLGRLKIPVYVVNPVTVADVLDLIARLGRLADRGDAADRTVTALRERM
                     QAVAVRVDGRPRPRVLYVLWPDPLIVPGRASLVSELIALAGGDSVTADGGQGYPRYSL
                     EAALARNPEVIILASHGSEKSPLMRDKWERFTQVPAIAAGRLHTIDGNLTHRYGPRIV
                     DGLERLARVIHPEAFDRTESR"
     gene            complement(1170..2243)
                     /gene="cobT"
                     /locus_tag="VNU03_17055"
     CDS             complement(1170..2243)
                     /gene="cobT"
                     /locus_tag="VNU03_17055"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWP76234.1"
                     /translation="MTALTKLRSLVTLPDPGAEVEVRRRLDQLTKPPGSLGRLEELVV
                     RLATIAGTCPPPLRDPVIFTLAGDHGVVAQGVSAYPQIVTAQMVENFLRGGAAVNVLA
                     RHASARVVVADLGVAAPLPRHPALRDLKIAPGTADMTRGPAMTRAQAVQAIEAGIGLV
                     EAERAAGADLVGTGEMGIGNTTAASALVAALTGATAESVTGPGTGVDAAGRRRKVEAI
                     RRALERNAPDPADALDTLAKVGGFEIAGLVGVILAGAAYRLPVVLDGFIAGAAALVAV
                     RLEPAARGALLAAHRSAEPGHARVLDALGLEPYLDLGMRLGEGTGAALCISLARAAVS
                     VLTDMATFKSAGVSDRADGPGAP"
     gene            complement(2218..2793)
                     /gene="cobU"
                     /locus_tag="VNU03_17060"
     CDS             complement(2218..2793)
                     /gene="cobU"
                     /locus_tag="VNU03_17060"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HWP76235.1"
                     /translation="MTAVHHSLLVLGGARSGKSRHALEQARQDGPRVAFLATARALDG
                     DMASRIARHRAERPPRWTTIEEPHEVGAACRRAARSHDLVVVDCVTVWVSNLMERGDD
                     DAVVLAAADDLAKLLRERLVSVILVSNEVGEGVHPPTELGRRFRDLLGLVNQRLAAAA
                     DRVTLMVAALPLTVKDAPLPTAPHDRSHEAP"
     regulatory      complement(2838..2969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3034..>3159)
                     /locus_tag="VNU03_17065"
     CDS             complement(3034..>3159)
                     /locus_tag="VNU03_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWP76236.1"
                     /translation="LYEDLTPPPPPREARPAPPAYRPPGAGRPTKRERRRIDRLR"
BASE COUNT          427 a         1159 c         1186 g          387 t
ORIGIN      
        1 gctcggcgag cgggttacgg gcgagggcct ggaaccccgc ccccgccacc gccagcgcgc
       61 cgcccgccag cagggcggcc aggatgcgcg gcagccgcag gctcagcacc acgacctgat
      121 cgaccccctt gatgcggccg gtgagcacgt tgagcacgtc tccgggcggc acgcgagcgc
      181 tgccgacgaa cagggcgccg agcgcggccg cgatcaccag gagcccgagc ccgcccagca
      241 ggacggcgag gcgcgcgccg gaggcggggg ccggctggct catcggctct ccgtccgatc
      301 gaaggcctcg gggtggatca ccctcgccag ccgctcgagg ccgtcgacga tgcgcggccc
      361 gtaccggtgc gtcaggttgc cgtcgatcgt gtggagccgc ccggccgcga tggccggcac
      421 ctgggtgaag cgctcccact tgtcgcgcat cagcgggctc ttctccgagc cgtggctcgc
      481 caggatgatg acctccgggt tgcgggccag ggcggcctcc aggctgtagc gcggatagcc
      541 ctgtcctccg tcggcggtca ccgagtcgcc gccggcgagg gcgatcagct cggacacgag
      601 cgaggcgcgc cccggcacga tgagaggatc gggccagagg acgtagagca cgcgcgggcg
      661 cggccggccg tccacccgca ccgccaccgc ctgcatccgc tcccgcagag cggtgaccgt
      721 ccggtctgcg gcgtcgcccc ggtcggcgag ccgcccgagg cgggctatca ggtccagcac
      781 gtctgccacc gtgaccgggt tgacgacgta gaccggtatc ttgagccgcc cgagctgctc
      841 gaacgtctcc tgtcgattgc cggcgatggt ggccacgacc agatcgggct tgagcgagac
      901 catgccctcg aggctcgggg cgagcatgcc gccgaccctg ggcttgcggc gcgcctccgc
      961 ggggtagtcg cagaagtcgg tcaccccgac caggcggtcc tgggccccga tcgagaagag
     1021 gatctcggtc acgctcggca cgagcgagat gatccgtccg ggcggcgcgg gcagtacgac
     1081 gcggcggccg gtctggtcgg tgacggtgag cgccccggcc ggggccggga gcagggtgag
     1141 cgccagcgcc agcgcggtca ggaaccggct cacggagccc cggggccgtc ggcgcggtcg
     1201 ctcacacccg ccgacttgaa ggtcgccatg tcggtgagca ccgagaccgc ggcgcgggcc
     1261 aggctgatgc agagcgccgc gccggtcccc tccccgagcc gcattccgag atcgagatac
     1321 ggctcgaggc cgagcgcatc gagcacccgt gcgtgcccgg gctccgccga ccgatgggcg
     1381 gcgagcagtg ccccccgcgc ggccggctcg agccggaccg cgaccagggc cgccgcgccc
     1441 gcgatgaagc cgtccaggac caccgggagg cggtacgcgg caccggccag gatgacccca
     1501 acgagaccgg cgatctcgaa gcctccgacc ttcgcgagcg tatcgagtgc atccgcggga
     1561 tccggcgcgt ttcgctcgag ggcacgccgg atcgcctcga ccttgcggcg gcggcccgcc
     1621 gcatccacgc ccgtgcccgg gccggtgaca ctctccgcgg tggcgccggt cagcgccgcc
     1681 accagcgcac tcgcggcggt ggtgtttccg atgcccatct cgccggtgcc gacgaggtcc
     1741 gcgcccgccg cccgctcggc ctcgacgagc ccgatgccgg cctcgatcgc ctgcaccgcc
     1801 tgggcgcgcg tcatcgcggg cccccgcgtc atgtccgcgg tgcccggcgc gatcttgagg
     1861 tcgcgcagag cgggatgccg gggcagcggg gccgccacgc ccaggtcggc caccaccacg
     1921 cgcgcggacg cgtgccgcgc gaggacgttg accgcggctc caccgcggag gaaattctcg
     1981 accatctgcg cggtgacgat ctgcggatac gcgctcacgc cctgggcgac cacgccgtga
     2041 tcgcccgcga gggtgaagat gaccggatcg cggagcggcg gcgggcaggt ccccgcgatg
     2101 gtggccaggc gcaccaccag ctcctcgagg cgcccgaggc tgcccggagg cttcgtcagc
     2161 tgatcgagcc ggcggcgcac ctcgacctcg gcgccgggat ccggcagggt cacgaggcta
     2221 cggagcttcg tgagagcggt catgcggggc ggtcggcagc ggggcgtcct tcacggtcag
     2281 ggggagcgct gccaccatca gggtcacccg atcggcggcg gcggcgagac gctgattgac
     2341 gagccccagc aggtcccgga agcggcggcc cagctcggtc ggcgggtgca cgccctcgcc
     2401 gacctcgttg gacaccagga tgacggagac gagacgctcg cgcagcagct tagccagatc
     2461 gtcggccgcg gccagcacca ccgcatcgtc gtcaccgcgc tccatgagat tcgagaccca
     2521 caccgtgacg cagtcgacga ccacgagatc gtgcgagcgg gctgcgcggc ggcaggccgc
     2581 ccccacctcg tgcggctcct cgatcgtggt ccaccgggga ggacgctcgg cccggtggcg
     2641 ggcgatgcgg ctggccatgt caccatcgag cgcgcgcgcg gtggcgagga acgcgacgcg
     2701 ggggccatcc tggcgggcct gctcgagcgc gtggcggctc ttgccgctac gggcaccgcc
     2761 caggacgaga agcgagtgat gcacggccgt cacccgggtc ctccctcgaa gaccggtacg
     2821 gggtggcggt gtcgggcagg tctcctggct ctcggatcat cctactcccc gccccttccc
     2881 gatccggctc gtgccggatc agtggccgat cgcgggtttc gttcccgatt acagtgacgg
     2941 ggtcgcggcg gattcgcacc gccttccctg gggcccgcac ccggcgggcc gcccggacac
     3001 catggacggc cagaccgtag cagaggggtc gggtcagcgc aaccggtcga tgcggcggcg
     3061 ctcgcgcttg gtggggcgtc ccgcacccgg cgggcggtag gcgggcggcg ccgggcgggc
     3121 ctcccgcggc ggcgggggcg gcgtgagatc ctcgtagag
//
LOCUS       DATMCV010000744         2146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_106752, whole genome shotgun sequence.
ACCESSION   DATMCV010000744 DATMCV010000000
VERSION     DATMCV010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106752"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2146)
                     /locus_tag="VNU03_17070"
     CDS             complement(<1..>2146)
                     /locus_tag="VNU03_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76237.1"
                     /translation="RYFEVESGDDASTIRDKVATRMRDLDPELEEGVPAILWVLGVAQ
                     DGGGLAAMDPGLRRRHVMRTVHRLVARQGRRQPFVMILEDMQWVDTETHAAMDALVET
                     LPPFVMVACTYRPEYADHWTGHPGFTPVRVGALEAPATDLLLDALLGTDPALAPLKRL
                     VAERANGNPLFLEECVASLVETGALEGEPGAYRFTRPTLALEVPATVRATIASRIDRL
                     RYEDKRLLQAASVIGDEVPVRLLEAVADVPVEEVQRGLEQLHAGGLLDQRALFPDLVY
                     GFRHALVHDVAYESLLHDRRRGLHARILDAIEQSYAGRLAEQTERLAHHAFLGEVWDR
                     AVGYCREAGARTLSRMASWESVSFFERALAALAHRPADAALGVDLRCDLHNALVPLGQ
                     HARSVAVLREAAALATSLGDQPRLARALSLESSVHWDMGASDEAARVGERALAITDEA
                     RDTGLWVVGHYNVGAGRRAVGDYRQALVVLRRVLAVLPPAASGERFGLPGIAAVLTRA
                     QLAWTLAELGQFDEAVQTAEEGLRLAEARRDPYSVTFALLGLGGTLVRRGQIREARGL
                     LERGIAQCGDMPAFYPPFAGDLAMINALTGRTAEALELAERGVRQAQSMQRLGRLSLI
                     MTHLGEARLLAGQPAAAAADARRALELAHTHRERGNVVYAERLLGLAARAASPPDAEA
                     ARGHFTRALDLAGLLGMRPLAARCHLGLARLAR"
BASE COUNT          266 a          801 c          832 g          247 t
ORIGIN      
        1 cgggcgagcc gggcgagccc caggtggcag cgggcggcca gcgggcgcat gccgagtagg
       61 ccggccaggt cgagcgcgcg cgtgaaatgg ccgcgggccg cctccgcgtc gggcgggctc
      121 gcggcgcggg cggccaggcc gagcagccgc tcggcgtaga ccacgttgcc ccgctcccgg
      181 tgggtgtggg ccagctcgag cgcgcgccgc gcgtcggccg ccgccgcggc gggctggccc
      241 gcgagcaggc gcgcctcgcc cagatgggtc atgatcagcg agaggcgtcc gagccgctgc
      301 atgctctggg cctgccgcac cccgcgctcg gccagctcga gcgcctccgc ggtccggccg
      361 gtgagcgcgt tgatcatcgc gagatccccc gcgaagggcg ggtagaacgc gggcatgtcc
      421 ccgcactgcg cgatgccgcg ctcgagcagt ccgcgggcct cgcggatctg gccgcggcgg
      481 acgagggtgc cgcccaggcc gagcagcgcg aaggtgacgc tgtaggggtc gcggcgcgcc
      541 tcggcgaggc gcagcccctc ctcggccgtc tgcaccgcct cgtcgaactg cccgagctcg
      601 gcgagggtcc aggcgagctg ggcgcgggtg aggaccgccg cgatgccggg caggccgaag
      661 cgctcgccgc tcgccgcggg cggcagcacc gcgagcacgc ggcgcaggac caccagggcc
      721 tgccggtagt cgccgaccgc ccgccggccc gctcccacgt tgtagtggcc gaccacccac
      781 agcccggtgt cccgggcctc gtcggtgatg gcgagcgcgc gctcccccac ccgcgcggcc
      841 tcgtcggagg cccccatgtc ccagtgcacg ctgctctcga gcgacagcgc gcgcgcgagt
      901 cgcggctggt cgccgaggct cgtcgcgagc gcggccgcct cgcgcagcac cgccacgctg
      961 cgggcgtgct ggccgagcgg gaccagcgcg ttgtgcaggt cgcagcgcag gtccaccccc
     1021 agggccgcgt cggcgggccg gtgggcgagc gcggcgaggg cccgctcgaa gaacgacacg
     1081 ctctcccagc tcgccatgcg cgagagcgtg cgggcgcccg cctcgcggca atagcccacc
     1141 gcgcggtccc acacctcgcc caggaacgcg tggtgggcga ggcgctcggt ctgctcggcc
     1201 aggcggcccg cgtaggactg ctcgatggcg tcgaggatgc gggcgtgcag gccccggcgc
     1261 cggtcgtgca gcaggctctc gtaggccacg tcgtggacga gggcgtggcg gaagccgtac
     1321 accagatcgg ggaatagcgc gcgctggtcg aggaggccgc ccgcgtggag ctgctcgagc
     1381 ccacgctgca cctcctcgac cggcacgtcg gccaccgcct cgagcaggcg caccggcacc
     1441 tcgtcgccga tcacggaggc ggcctggagc aggcgcttgt cctcgtagcg gagccggtcg
     1501 atgcgggagg cgatggtggc gcgcacggtg gccggcacct ccagcgcgag cgtgggacgg
     1561 gtgaaccggt acgcgccggg ctcgccctcg agcgcgccgg tctccaccag gctggccacg
     1621 cactcctcga ggaagagcgg gttaccgttc gcgcgctcgg ccacgagccg cttgagcggg
     1681 gcgagcgcgg gatcggtgcc gagcagagcg tccagcagca ggtcggtggc cggcgcctcg
     1741 agcgcgccca cccgcaccgg ggtgaagccc gggtgtccgg tccagtggtc cgcgtactcc
     1801 gggcgatagg tgcacgccac catcacgaag ggcggcaggg tctccaccag cgcgtccatc
     1861 gcggcgtgcg tctcggtgtc cacccactgc atgtcctcga ggatcatcac gaagggctgg
     1921 cgccggccct ggcgcgccac caggcggtgg acggtgcgca tgacgtggcg gcggcggagg
     1981 ccggggtcca tggccgcgag gccgcccccg tcctgcgcca cgcccagcac ccacaggatg
     2041 gccggcacgc cctcctcgag ctcgggatcg agatcgcgca tccgggtggc caccttgtcg
     2101 cggatggtcg acgcgtcgtc gccgctctcc acctcgaagt agcggc
//
LOCUS       DATMCV010000745         5213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_37508, whole genome shotgun sequence.
ACCESSION   DATMCV010000745 DATMCV010000000
VERSION     DATMCV010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37508"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1266)
                     /gene="pdhA"
                     /locus_tag="VNU03_17075"
     CDS             complement(73..1266)
                     /gene="pdhA"
                     /locus_tag="VNU03_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HWP76238.1"
                     /translation="MPRTQIEPQFTVEHLSILDSDGNLDSALEPSIAPDELKRLHRAM
                     LLGRRFDERMLRLQRQGRIGTFAPIKGQEAAQIGSVAALRRTDWMVPSFRETASMLWR
                     GWTMERILLFFAGYLEGGQPGPEQRDLPICIPVATQLPHAVGLAYAAQYRGDDAVVMA
                     YCGDGATSEGDFHEAMNFAGVWHVPMVFLIQNNQWAISVPLKKQTHSRTIAQKALAYG
                     FPGLQVDGNDLLAVYSATREAVERARRGEGPTLIECVTYRLGVHTTSDDPTKYRSTEE
                     VQAWERKDPLTRFGAYLQKRNLLEAGLEEAVDAEIARAVSAFEATGAADPLAMFDHAY
                     ATRTAPLEAQRAEMQERLARAPDGVPGPVAPAPPASAAGDAAGTGASDGQSAPMRGQR
                     RPWRS"
     gene            1345..2256
                     /gene="thrB"
                     /locus_tag="VNU03_17080"
     CDS             1345..2256
                     /gene="thrB"
                     /locus_tag="VNU03_17080"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWP76239.1"
                     /translation="MRVRVRVPATSANLGPGFDALGLALALYNEVTLEEADRVSVAVE
                     GEGSGRLDEGAKNVVARGAALAFEIAGRAFPGVRLRCVNRIPLSRGLGSSAAAWVGGL
                     VAANALMGEPLDQDALLAAATRAEGHPDNVAAAILGGLTVSCADGPRVAAVSLSVPRE
                     IEWVVLVPETESSTREARAVLPESVPRADAIFNVQRVSLFLAALAAGRAELLALAMQD
                     RLHQPYRLRLFPWMDAVAAAGREAGALGCVLSGAGPAMLAAVRPRGGESVAQAMEQAL
                     RGAGIAGRALHLSVEASGATWERLPQT"
     gene            2300..2851
                     /gene="dcd"
                     /locus_tag="VNU03_17085"
     CDS             2300..2851
                     /gene="dcd"
                     /locus_tag="VNU03_17085"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWP76240.1"
                     /translation="MIKSDRWIRRQALEKGMIKPFEDRQVRKGGISFGLSSYGYDIRI
                     ADEFKIFTNINTTIVDPKAFDPRSFVDYQGEVALIPPNSFALGRSVEYFKIPRNVLTI
                     CVGKSTYARCGIITNVTPFEPEWEGYVTLEISNTTPLPAKIYANEGIAQVLFFESDEA
                     CETSYADRQGKYQGQQGIVLPTV"
     gene            2898..3104
                     /locus_tag="VNU03_17090"
     CDS             2898..3104
                     /locus_tag="VNU03_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HWP76241.1"
                     /translation="MAEQKKKETRPTKPNEGGGDDAGAGSADLSKKGKKIKEELDNLL
                     DEIDDILEENAEEFVKSYVQRGGQ"
     gene            3138..3356
                     /locus_tag="VNU03_17095"
     CDS             3138..3356
                     /locus_tag="VNU03_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWP76242.1"
                     /translation="MPIYEYGCLACGAHFEKFMQVATAVACPSCESDRVSRRLSVVGV
                     KVGGRASDPSAAPMGGGGGCCGGGCGCH"
     gene            3475..4368
                     /locus_tag="VNU03_17100"
     CDS             3475..4368
                     /locus_tag="VNU03_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HWP76243.1"
                     /translation="MDLRQLEIFVKVAELGSFSRAAQALFLTQPTVSEHIRTLEDELG
                     VRLLDRLGRGAAVTKGGALLLGYAQRMLALSREARQAMESFQGRMSGDLLVGASTIPG
                     EYILPALIGRFKEKFPDIAITLLIGGSQAVTEWVLEGRAEVGVVGARAGHRAIEYREL
                     FPDDIVLIVGATHPWQGRPLVTLDELRAEPLLLREQGSGTRAALESALAAAGTDIGAF
                     RVVGEMGSTQAIKQAVKAGVGVSMVSRRAVEEECRAGSVWCLRVRDLTITRAFYLATH
                     RERSRSPLAEAFRAFVETEAS"
     gene            4505..>5213
                     /gene="selD"
                     /locus_tag="VNU03_17105"
     CDS             4505..>5213
                     /gene="selD"
                     /locus_tag="VNU03_17105"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HWP76244.1"
                     /translation="MLGDLRHEEHADLLVGLGRSDDAAVYRVTDDVAIVETVDFFPPI
                     VDDPYLYGAIAAANSMSDVYAMGGQVLFALNVAGFPRDLPKAIIAAVFRGGADKVREA
                     GGVIAGGHTVVDAEPKYGLCVTGRVDPRRILLKGGLRPGQQVFLSKPLGTGVIATAAK
                     NDKCEAAVLDGAVQSMLRLNRAAAEVAQEAGAGGATDITGFGLLGHASEMVEASGAGL
                     TLRSRDLPLLPGALALAE"
BASE COUNT          722 a         1806 c         1913 g          772 t
ORIGIN      
        1 cgccgatgac gagcacgtcg gggtcgcgct ccatctcctg caggagcgcc aggttgaggg
       61 ccttcaccat gttcagcttc gccacggtcg cctctgtccc cgcatgggcg cgctctgacc
      121 gtcgctcgcc ccggtcccgg ccgcgtcccc cgctgccgat gcgggcggcg ccggcgcgac
      181 cggccccggc acgccgtcgg gcgcccgggc gaggcgctcc tgcatctcgg cccgctgcgc
      241 ctcgagcggg gcggtccgcg tggcgtaggc gtggtcgaac atggcgagcg ggtcggcggc
      301 gccggtcgcc tcgaaggcgg agacggcgcg cgcgatctcc gcgtccaccg cctcctcgag
      361 gccggcctcg aggagattcc gcttctggag atacgcgccg aagcgggtga ggggatcctt
      421 ccgctcccac gcctgcacct cttccgtcga gcggtacttg gtggggtcgt cggaggtggt
      481 gtgcacgccc agccgatacg tcacgcactc gatcagcgtc ggcccctcgc cgcggcgcgc
      541 ccgctccacc gcctcccgcg tcgcgctgta gaccgcgagc aggtcgttgc cgtcgacctg
      601 cagccccggg aacccgtagg cgagcgcctt ctgggcgatg gtgcgcgagt gggtctgctt
      661 cttgagcggg accgagatcg cccactggtt gttctggatg aggaacacca tcggcacgtg
      721 ccagaccccc gcgaagttca tcgcctcgtg gaagtcgccc tccgaggtgg ccccgtcgcc
      781 gcagtaggcc atcaccaccg cgtcgtcgcc gcgatactgc gccgcgtagg ccaggcccac
      841 cgcgtgcggc agctgggtgg cgaccgggat gcagatgggc agatcgcgct gctccggccc
      901 cggctgcccg ccctcgaggt agccggcgaa gaacagcagg atcctctcca tggtccagcc
      961 gcgccagagc atggaggcgg tctcccggaa ggacggcacc atccagtcgg tgcgccgcag
     1021 ggccgcgaca ctgccgatct gggcggcctc ctggcccttg atgggcgcga acgtgccgat
     1081 gcgcccctgg cgctgcagcc gcagcatgcg ctcgtcgaag cggcggccga ggagcatcgc
     1141 gcgatggagg cgcttgagct cgtcgggggc gatcgagggc tcgagggcgg agtccaggtt
     1201 gccgtcgctg tccaggatgg acaggtgctc gacggtgaac tgcggctcga tctgggtcct
     1261 cggcatggcg gccggctcgt ttccctcgca ttctaccccg gtccgctcta ggcggcctcc
     1321 gcgaaagcgg gtaatctctc tgccatgcgc gtccgggtgc gggtgcccgc cacctcagcc
     1381 aacctcggtc ccggattcga cgcgctgggc ctggcgctgg cgctctacaa cgaggtcacg
     1441 ctcgaggagg ccgaccgggt ctcggtcgcg gtggagggcg aggggagcgg gcggctcgac
     1501 gagggcgcca agaacgtcgt cgcgcgtggg gccgcgctgg ccttcgagat cgcgggccgc
     1561 gcgtttccgg gcgtgcgcct ccgctgcgtc aaccgcatcc cgctgagccg cggcctgggc
     1621 tcgagcgcgg ccgcgtgggt cggcgggctg gtcgcggcca acgcgctcat gggcgagccg
     1681 ctcgaccagg acgcgctgct cgccgccgcc acccgcgcgg aggggcaccc ggacaacgtg
     1741 gccgccgcga tcctcggcgg gctcaccgtc tcctgcgcgg acggcccgcg cgtcgccgcg
     1801 gtgagcctgt cggtgccgcg cgagatcgag tgggtcgtcc tggtccccga gaccgagagc
     1861 tcgacccgcg aggcgcgcgc ggtgctgccc gagtcggtgc cccgggccga cgcgatcttc
     1921 aacgtgcagc gcgtgagcct cttcctggcc gcgctcgccg cgggccgcgc ggagctgctc
     1981 gcgctggcga tgcaggaccg gctccaccag ccctatcgcc tccggctctt cccgtggatg
     2041 gacgcggtgg ccgcggcggg gcgcgaggcc ggcgcgctcg gctgcgtcct gagcggagcg
     2101 gggcccgcca tgctcgccgc ggtgcggccg cgcggtgggg aatcggtcgc ccaagccatg
     2161 gagcaggccc tccgcggtgc cgggatcgcg ggtcgggcgc tccacctgtc ggtcgaagcg
     2221 agcggcgcga cgtgggaacg gcttccccag acttgactcg ccctctcggc aggcagtacg
     2281 atccgtgcaa ggtgtatccg tgatcaagag tgaccgctgg atcaggcggc aagccctgga
     2341 gaagggcatg atcaagccct tcgaggaccg ccaggtccgc aagggcggga tttccttcgg
     2401 gctgtcctcc tatggctacg acatccgcat cgcggacgag ttcaagatct tcaccaacat
     2461 caacaccacc atcgtggacc cgaaggcctt cgacccgcgc tccttcgtcg attaccaggg
     2521 tgaggtggcg ctgatcccgc ccaactcctt cgcgctgggg cggtcggtcg agtacttcaa
     2581 gatcccccgc aacgtgctga cgatctgcgt ggggaagtcc acctatgcgc ggtgcggcat
     2641 catcacgaat gtgaccccgt tcgagccgga gtgggagggg tacgtgaccc tcgagatcag
     2701 caacaccacc ccgcttccgg ccaagatcta cgccaatgag ggcatcgcgc aggtcctatt
     2761 ttttgaatca gacgaggcct gcgagacgtc atatgctgac aggcagggga agtatcaggg
     2821 ccagcagggg atcgtgctcc cgaccgtcta ggccctgaga ccggtcccgc gaacgcgggg
     2881 ccaggaggtg acgtccgatg gctgagcaga agaagaagga gacgaggcca accaagccca
     2941 acgagggcgg tggcgacgac gccggcgcgg gaagcgccga cctgtccaag aagggcaaga
     3001 agatcaagga agagctggat aacctgctgg acgagatcga cgatatcctc gaggaaaacg
     3061 ccgaggagtt cgtgaagagc tacgtccagc gcggcggcca gtagccgcgc gtcagcccga
     3121 ggagccggag gcgtaccatg ccgatctacg aatacgggtg cttggcctgc ggagcgcatt
     3181 tcgagaagtt catgcaggtg gcgaccgcag tggcctgccc gtcgtgcgag agcgatcgcg
     3241 tcagccgtcg tctctcggtg gtcggcgtga aggtgggcgg acgcgcgagc gatccgtcgg
     3301 ccgcgcccat gggtgggggc ggagggtgct gcggcggcgg ctgcggctgt cactgatcgc
     3361 cgccacgcgt cctgcgtgag tccggggggc cttcgggccc ctttgttgtt cccgagtcgc
     3421 tccggctgac cgccctcgct ggacagcgcc ccgcctcgct cctatactcg cctcgtggat
     3481 ctacgccagc tcgagatctt cgtgaaggtt gccgagctcg gctcgttctc ccgggccgcc
     3541 caggccctct tcctgaccca gccgacggtc tccgagcaca tccggaccct ggaggacgaa
     3601 ctcggggtgc gcctgctcga ccggctcggc cgcggggccg cggtcaccaa ggggggcgcg
     3661 ctgctgctgg gctacgcgca gcgcatgctc gcgctgtcgc gcgaggcgcg gcaggcgatg
     3721 gagagcttcc aggggcggat gagcggagac ctcctggtgg gggccagcac catcccgggg
     3781 gagtacatcc tcccggccct gatcggccgc ttcaaggaga agttcccgga catcgccatc
     3841 accctgttga tcggcggcag ccaggcggtc accgagtggg tgctggaggg ccgggccgag
     3901 gtgggcgtgg tgggcgcgcg cgcgggccac cgcgcgatcg agtaccgcga gctgttcccc
     3961 gacgacatcg tgctcatcgt gggcgcgacg catccctggc aggggcgccc gctggtgacg
     4021 ctcgacgagc tgcgcgccga gccgctgctg ctgcgcgagc aggggtcggg cacgcgcgcc
     4081 gcgctcgagt cggccctggc cgccgcgggc accgacatcg gcgcgttccg ggtcgtgggc
     4141 gagatgggct cgacccaggc gatcaagcag gcggtcaagg ccggggtcgg ggtgtcgatg
     4201 gtgtcacggc gggcggtcga ggaggagtgc cgcgcgggca gcgtgtggtg cctgcgcgtc
     4261 cgcgacctca cgatcacccg cgccttctac ctcgccacgc atcgggagcg gagtcgctcc
     4321 cccctcgccg aggccttccg ggccttcgtc gagaccgagg ccagctgacg ccgcgctccc
     4381 gtcggtcccg cccccgatgg atccccgcct gatcgatccg aagaccggca agcagatccg
     4441 gctcaccgcc tacgcggcct gcgccgggtg agcgtcgaag atgggtcctg gagatctcca
     4501 ggacgtgctg ggcgacctcc gacacgagga gcacgccgat ctcctggtcg gcctcggccg
     4561 gagcgacgac gccgcggtct accgggtgac cgacgacgtg gcgatcgtcg agacggtgga
     4621 tttcttcccg cccatcgtgg acgacccgta tctctacggc gcgatcgcgg cggcgaactc
     4681 gatgtccgac gtctacgcca tgggcgggca ggtgctcttc gccctcaacg tggccggctt
     4741 cccgcgcgac ctgccgaagg cgatcatcgc ggcggtcttc cgggggggcg ccgacaaggt
     4801 gcgcgaggcg ggcggcgtca tcgcgggcgg gcacacggtg gtggacgccg agcccaagta
     4861 cggcctctgc gtcaccggcc gcgtggaccc gcgccggatc ctgttaaagg gcggcttgcg
     4921 tccggggcag caggtcttcc tctcgaagcc gctcggcact ggagtgatcg ccaccgcggc
     4981 caagaacgac aagtgcgagg cggcggtgct ggacggcgcg gtgcagagca tgctccggct
     5041 gaaccgggcc gcggccgagg tcgcgcagga ggcgggcgcg ggcggcgcga ccgacatcac
     5101 cggcttcggc ctgctcggcc acgcgagcga gatggtggag gcctccggcg ccgggctcac
     5161 gctgcggagc cgcgacctgc cgctcctgcc gggcgcgctc gcgctcgccg agc
//
LOCUS       DATMCV010000746         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_836604, whole genome shotgun sequence.
ACCESSION   DATMCV010000746 DATMCV010000000
VERSION     DATMCV010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836604"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1308)
                     /locus_tag="VNU03_17110"
     CDS             complement(34..1308)
                     /locus_tag="VNU03_17110"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWP76245.1"
                     /translation="MSLLIRNGRVVDPAGGTDAVADVLIADGRIQKVGRALKAPAGTE
                     LIDATGKVVCPGFIDMHVHLREPGFEYKETIASGTRAAAAGGFTAVACMANTFPVNDN
                     RAVTDYILARAKVEGVVRVYPIGAVTRNLEGRQLAELAEQAEAGCVAFSDDGKCVMNA
                     ELYRRAMEYALPFGTPIISHAEDCHLSHGAHMNEGLVATELGLSGQPGAAEDVMVARD
                     IILAELTGAHLHIAHISTAGAVRLVREARARGVRVTAEVTPHHLVLTDEAVRSYDPNC
                     KMAPPLRTKRDVEACLEGLIDGTIECVATDHAPHATSEKEGEFAEAANGIVGLETAVP
                     ILLDRLVRPGVVDLPTLVARLSTGPARLLNLPGGSLAPGAAADVTILDLERAFTVDPA
                     AFQSRSRNTPFAGFTGTGTPWMTLVGGVKVTP"
     gene            complement(1305..>2230)
                     /locus_tag="VNU03_17115"
     CDS             complement(1305..>2230)
                     /locus_tag="VNU03_17115"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWP76246.1"
                     /translation="SWKRKDLLGMQDLDATEIIGVLDTASSMKEIAAREIKKVPTLRG
                     KTVVNLFYESSTRTRTSFEIAGKWLSADVINFSASGSSSDKGESLLDTARNIEAMSPD
                     VVVVRHKASGAPALLARHLRCGVVNAGDGAHEHPTQALLDLMTIREKKGHLEGLNVTI
                     VGDVTHSRVARSDIFGMRKMGITVTVAGPPTLIPAQCQELGVKVSHRLEEAIASADVI
                     MMLRLQHERMEGGFIPSIREYSRVWGLSLDKLKPCRPDVLIMHPGPVNRGVELSPEVA
                     DSPFSVILDQVGNGVAVRMAVLYLLAGSKGA"
BASE COUNT          302 a          773 c          809 g          346 t
ORIGIN      
        1 gcccgtcggc gagcgcgagc agggccgggc gcgtcacgga gtcaccttga cgccgccgac
       61 gagcgtcatc cacggcgtgc cggttccggt gaagccggcg aagggcgtgt tccggctccg
      121 cgactggaac gcggccggat ccaccgtgaa ggcccgctcg agatcgagga tggtgacgtc
      181 ggccgccgcc ccgggggcga ggctgccgcc gggcaggttc agcaggcgcg cgggcccggt
      241 gctgagccgg gcgaccaggg tgggcagatc gaccacgccc ggccgcacca gacggtccag
      301 caggatcggc accgcggtct cgagcccgac gatgccgttg gccgcctccg cgaactcgcc
      361 ttccttctcg gaggtggcgt gcggggcgtg gtccgtggcc acgcactcga tggtgccgtc
      421 gatgaggccc tccagacacg cctcgacgtc gcgcttcgtg cggaggggcg gcgccatctt
      481 gcagttggga tcgtagctgc gcaccgcctc gtcggtgagc accaggtggt gcggggtcac
      541 ctccgcggtg acccggaccc cgcgcgcccg ggcctcgcgc acgaggcgca ccgcgcccgc
      601 ggtcgagatg tgcgcgatgt gcaggtgggc gccggtcagc tcggccagga tgatgtcgcg
      661 cgccaccatc acgtcctcgg ccgcgccggg ctggccggag agccccagct cggttgcgac
      721 gaggccctcg ttcatgtgcg ccccgtggga caggtgacag tcctccgcgt ggctgatgat
      781 cggggtgccg aagggcagcg cgtactccat ggcgcggcgg tagagctccg cgttcatcac
      841 gcacttgccg tcgtcggaga aggcgacgca cccggcctcg gcctgctcgg ccagctcggc
      901 gagctgccga ccctcgaggt tgcgagtcac cgcgccgatc gggtacacgc ggacgacgcc
      961 ctcgaccttc gcgcgggcca ggatgtagtc ggtgaccgcg cggttgtcgt tgaccgggaa
     1021 ggtgttggcc atgcaggcca ccgcggtgaa cccgcccgcg gcggcggcgc gggtgccgga
     1081 ggcgatcgtc tccttgtact cgaagcccgg ctcgcggagg tgcacgtgca tatcgatgaa
     1141 gcccgggcag acgacttttc cggtggcgtc gatgagctcg gtgcccgcgg gcgccttgag
     1201 ggcgcggccg accttctgga tccgcccgtc cgcgatcagg acgtccgcca ccgcgtcggt
     1261 gccgccggcc ggatcgacca cgcgcccgtt ccggatcagc aggctcatgc gcccttgctc
     1321 ccggccagga ggtagagcac cgccatccgc accgcgaccc cgttgccgac ctggtcgagg
     1381 atcaccgaga aggggctgtc ggccacctcg ggcgagagct cgaccccgcg gttcaccgga
     1441 ccggggtgca tgatgagcac gtcgggccgg cagggcttga gcttgtcgag cgagagcccc
     1501 cacacccgcg agtactcgcg gatggacggg atgaagccgc cctccatccg ctcgtgctgc
     1561 aggcgcagca tcatgatcac gtccgcgctg gcgatggcct cctccagccg gtggctcacc
     1621 ttcacgccca gctcctggca ctgggccggg atcagggtgg gcgggcccgc caccgtcacc
     1681 gtgatgccca tcttgcgcat cccgaagatg tcggagcggg cgacccggct gtgggtgacg
     1741 tcgcccacga tcgtcacgtt gagcccttcg agatggcctt tcttctcccg gatcgtcatg
     1801 aggtcgagca gggcctgggt cgggtgctcg tgggcgccgt cgcccgcgtt gaccactccg
     1861 cagcggaggt gccgcgccag cagggcgggg gccccggagg ccttgtgacg taccaccacc
     1921 acgtcggggc tcatggcctc gatgttgcgg gcggtgtcga gcaggctctc gcccttgtcc
     1981 gagctcgaac cggaggcgga gaagttgatc acgtcggccg agagccactt gcccgcgatc
     2041 tcgaacgagg tgcgggttcg ggtggacgac tcgtagaaca ggttcaccac cgtcttgccc
     2101 cggagggtcg ggaccttctt gatctcgcgg gccgcgatct ccttcatgga ggacgcggtg
     2161 tcgagcacgc cgatgatctc ggtcgcgtcc aggtcctgca tgccgagcag atccttgcgc
     2221 ttccagctca
//
LOCUS       DATMCV010000747         5431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_174912, whole genome shotgun sequence.
ACCESSION   DATMCV010000747 DATMCV010000000
VERSION     DATMCV010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174912"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VNU03_17120"
     CDS             complement(<1..1173)
                     /locus_tag="VNU03_17120"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76247.1"
                     /translation="MASRSFLVLRLISLLGAALWLGTAGPAVAAPEGTLTIAMHFSPV
                     TRWLDPAEGESTITPYLLLAALHDGLLRPTAGQASAPSLAESWAMAKDMTSAEFTLRK
                     AKFHNGDPVTAEDVKFSFERYRGGAAKTLKDGVKEIQVLGPSRVRFVFKGPWPDFPAF
                     YGTFVASAGWVVPKKYFERVGEEGYRKAPVGAGPYKFVSFNPGVELVVEAFDGYWRKQ
                     PSIKRIVFRSLPDETTRAAALKSGEVDLAMLLSGPTAQEIKRTPGLRLAAPLLGVFWL
                     DFPDQWDPKSPWADRRVRQAASLALDRQALNEAETLGLSRPVGNTVPRDYEFALPVEP
                     PPYNPARARQLLAEAGYPNGFDAGELTPFPPYNSMGETIQGWLQAVGIRTRVRAMER"
     gene            complement(1229..1957)
                     /locus_tag="VNU03_17125"
     CDS             complement(1229..1957)
                     /locus_tag="VNU03_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76248.1"
                     /translation="MPAGHSMQSRAWPLAVLWLTLALVAPGRVAAQAGSPSTASLLDF
                     VTFDGIDYIRFLDEPGRALTRDDLGIEFATVACSIGEDNRTCSYGLDGGAAFLPAGTK
                     VYAVRGYATEFRLAAVWRDHVYLYQAWRNPRARVGAALFDIAGKVRTIEVRLGEPTAE
                     APGTPAVIAARADVDALVDMIGRGTARRPLPHAVGETRYWLTFWLADGTTLGRAYFPE
                     ASELMGGVIVPGEFRALLDRYLAR"
     gene            2015..2623
                     /locus_tag="VNU03_17130"
     CDS             2015..2623
                     /locus_tag="VNU03_17130"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HWP76249.1"
                     /translation="MKTVVACETCGLVQRLEGLGPGLVAECARCGAQLADHKPNSLAR
                     TAAFSLAALLLYVPANIFPILEMDLYGVHSENTVWEGCKTLFQHGEILVAVVVFMASL
                     LIPALKLLGLFFLVVTTRRGSNRRRPVRTWVYRTIDRVGPWAMLDVFLLAILVALVKL
                     GELATVIPGPGLLAFTAVVVLTILASSSFDPTLIWADRGDRP"
     gene            2620..3531
                     /locus_tag="VNU03_17135"
     CDS             2620..3531
                     /locus_tag="VNU03_17135"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWP76250.1"
                     /translation="MTDPSPEQPAPEPGPDGPLPTATVRKRRWRFPVVWVVPLIAAIV
                     AATLVYNRLQEFGPTITIKFRDGSGVKAGQTEIRYRGVPVGQVTTIELTLDHEHVVVT
                     ARLRHTAAALAKEGALFWIVRPEVGFGTVRGLSTVLTGAYIQSLPGRGKDRKDFTGLE
                     SASPTLGQAGLTVTLAARQVGSVRIGSPVFYRGIEVGSVIAADLGPDATAAQIKVFVP
                     QRYARLVRLGSRFWLQSGVDVNFGLFRGLEVNVESLRSLVAGGIAFATPDDAGGPVKD
                     GTLFLLHEKPQNEWLDWMPKIPIAPAG"
     gene            3533..4954
                     /locus_tag="VNU03_17140"
     CDS             3533..4954
                     /locus_tag="VNU03_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HWP76251.1"
                     /translation="MRALVTLLVLTLLAPAAAFAESPKLDWETGEGKSYVIPALEIGG
                     FLLGLNQFNRHVIDSKEYGSDFKSIGNNLTGPLVIDRDPFSVNQLGHPYQGNMYFGFA
                     RSAGLDFWQSLAYSLGGSLLWETAGETTPPSLNDHINTAIGGAFIGEAAFRMASLLLE
                     GGGQNPGFWRELGAAVLSPPTGFNRLVFGDRFDKVFPSRNPEVFIRLRLGATLTNEVN
                     NDIKKDVKRQEGAADYSITYGLPGKPGYEYKRPFDYFHFEFTAVPNAGDITDAIENVS
                     IRGLLGGRRYTLGDDFRGVWGVFGNYDYFSPQIFRVSSTAVSMGTVFQWWLSRHVALQ
                     GIALGGLGLGAAGTVGDRAEREYHYGLTPLTLLGMRLIFGDRAMLEATGRQYYVAGTS
                     SNRETGVDTSHFSQDVILRGSLGFTVRLWGPHALRVQYLISSRNTGAPDLRDRHQSVQ
                     TVTISYNFLGHTRFGAVEWRPDQ"
     gene            4964..>5431
                     /locus_tag="VNU03_17145"
     CDS             4964..>5431
                     /locus_tag="VNU03_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76252.1"
                     /translation="MLGPTLAPTLAKIVAFGLFLALGFAAIPLMLRVFLWGQRRIGNE
                     AVPMVRFLADHQTGVVVAVWIVFALGLGIALPAMLADEFFGRPAKLWFEARLRGASRG
                     VLTANVGMTLEDIRRRSTLPMSEPRGESLTGSSRLAADVVFDLDIADTGTLFPG"
BASE COUNT          767 a         1943 c         1880 g          841 t
ORIGIN      
        1 gcgctccatg gcgcggaccc gcgtgcggat gccgaccgcc tggagccaac cctggatggt
       61 ctcgcccatc gagttgtagg gcgggaacgg ggtcagctcg cccgcgtcga agccgttcgg
      121 gtagccggcc tcggccagca gctggcgggc gcgggcggga ttataaggag gcggctccac
      181 cggcagcgcg aactcgtagt cgcgcgggac ggtgttcccg accgggcgcg agagccccag
      241 cgtctccgcc tcgttcaggg cctggcggtc cagcgccagg ctcgcggcct gccgcacgcg
      301 cctgtccgcc cagggcgact tcggatccca ctggtcgggg aagtctagcc agaacacgcc
      361 gagcagcggc gcggccaggc gcaggcccgg cgtccgcttg atctcctgcg cggtggggcc
      421 gctcagcagc atggccaggt cgacctcgcc gctcttgagc gcggccgcgc gcgtcgtctc
      481 gtccggcagg ctgcggaaga ctatcctttt tatggacggc tgctttcgcc agtagccgtc
      541 gaacgcctcg acgaccagct ccacgccggg gttgaagctc acgaacttgt agggcccggc
      601 cccgaccggc gccttgcgat agccttcctc gccgacgcgc tcgaagtact tcttcggcac
      661 cacccagccc gcgctcgcca cgaaggtgcc gtagaacgcg gggaagtcgg gccacggccc
      721 cttgaacacg aaccgcacgc ggctcgggcc gagcacctgg atctccttca cgccgtcctt
      781 cagggtcttg gccgcgccgc cgcgatagcg ctcgaaggag aacttcacgt cctccgcggt
      841 gaccgggtcc ccgttgtgga acttcgcctt tcgcagggtg aactcggcgc tcgtcatgtc
      901 cttcgccatg gcccacgact cggccaggct gggcgcggag gcctgtcccg cggtgggccg
      961 gagcagcccg tcgtgcaggg cggccagcag gagatagggc gtgatcgtgc tctcgccctc
     1021 ggccggatcg agccagcgcg tgaccgggct gaagtgcatc gcgatcgtca gcgtgccctc
     1081 cggggcggcg accgcggggc cggcggtacc gagccagagc gcggcgccga ggagggagat
     1141 cagccggagg acgaggaagg accgggaggc catgcctgga tggagcgcat tctagtgcaa
     1201 gccgggcgat cgcacaaacc ctcggccctc accgggccag gtagcggtcg agcagggcgc
     1261 ggaactcgcc gggcacgatc acgccgccca tcagctcgct cgcctcgggg aagtaggcgc
     1321 gaccgagcgt cgtgccgtcc gcgagccaga aggtgagcca gtagcgggtc tcgcccaccg
     1381 cgtgcggaag cggccggcgc gcggtcccgc gcccgatcat gtcgaccagg gcgtccacgt
     1441 ccgcgcgcgc ggcgatcacc gccggcgtcc cgggtgcctc cgcggtcggc tcgccgagcc
     1501 gcacctcgat ggtgcgcacc ttgcccgcga tgtcgaagag cgccgcgccg acccgcgcgc
     1561 gcggattgcg ccaggcctgg tagaggtaga cgtgatcccg ccacaccgcg gcgagccgga
     1621 actcggtcgc gtagccgcgc accgcgtaca ccttcgtgcc ggcgggcagg aaagcggcgc
     1681 cgccgtcgag gccgtacgag caggtgcggt tgtcctcgcc gatcgagcag gcgaccgtcg
     1741 cgaactcgat gccgaggtcg tcgcgggtca gcgcccggcc gggctcgtcc aggaagcgga
     1801 tgtagtcgat gccgtcgaag gtgacgaagt cgagcaggct cgcggtgctc ggcgagccgg
     1861 cttgcgcggc gacgcgcccg ggcgcgacga gcgccagcgt cagccagaga acagcgagcg
     1921 gccaggctct gctctgcatc gagtgaccgg caggcatgat acgcctcgcg gcggggcggg
     1981 cctcggaatt gcacgacggc ggcctaccgg catcgtgaag accgtcgtcg cctgcgagac
     2041 ctgtggcctg gtgcagcgcc tggaggggct cggccccggc ctggtggccg agtgcgctcg
     2101 ctgtggcgca cagctcgcgg accacaagcc caacagcctc gcccgtaccg ccgcgttttc
     2161 gttggccgcg ctcctcttgt acgtgccggc caacatcttc cccatcctcg agatggacct
     2221 ttacggcgtc cactcggaaa acacggtgtg ggaggggtgc aagacgctct tccagcatgg
     2281 cgagattctg gtcgcggtgg tggtcttcat ggcgagcctg ctcatcccgg ccctcaaact
     2341 actcggtctt ttcttcctgg tcgtgaccac tcggcgcggc tccaaccgcc ggcggccggt
     2401 ccggacgtgg gtctaccgca cgatcgatcg ggtcggaccg tgggccatgc tcgacgtgtt
     2461 cctgctcgcg atcctggtcg cgctggtcaa gctgggcgag ctggccaccg tgatcccggg
     2521 gcccgggctg ctcgccttca ccgcggtggt cgtgctgacc atcctggcct cgagcagctt
     2581 cgacccgacc ctcatctggg ccgatcgcgg cgatcggccg tgaccgatcc ctcgccggag
     2641 cagccggccc cggagcccgg gcccgacggt cccctgccga ccgcgacggt ccgcaagcgg
     2701 cggtggcggt tcccggtcgt ctgggtcgtc ccgcttatcg ccgccatcgt ggcggccacc
     2761 ctcgtctaca atcgcctgca ggagttcggg ccgaccatca cgatcaagtt ccgggacggc
     2821 agcggcgtga aggccggtca gaccgagatc cgctaccgcg gcgtgccggt gggccaggtc
     2881 acgacgatcg agctgaccct ggatcacgag cacgtggtgg tgaccgcacg gctgcgccac
     2941 accgcggccg cgctcgccaa ggagggcgcg ctgttctgga tcgtccgtcc cgaggtcggt
     3001 ttcggcaccg tgcgcggtct cagcaccgtg ctcaccggcg cctacatcca gtcgctgccg
     3061 ggccgcggca aggatcgcaa ggacttcacc ggcctcgaga gcgcttcgcc cacgctcggc
     3121 caggcggggc tcaccgtgac cttggccgcg cggcaggtcg ggtcggtgcg gatcggctcg
     3181 cccgtcttct accgagggat cgaggtgggc tcggtcatcg cggcggatct ggggcccgac
     3241 gccaccgcgg cgcagatcaa ggtgttcgtc ccgcagcgat acgcccggct ggtgcggctc
     3301 ggctcgcgct tctggctgca gagcggcgtg gacgtcaact tcggcctctt ccgcggcctc
     3361 gaggtcaacg tcgagtcgct acgctcgctg gtggcgggcg gcatcgcgtt cgccacgccg
     3421 gacgatgcgg gcggcccggt gaaggacggc accctgttcc tgctgcacga gaagccgcag
     3481 aacgagtggc tcgactggat gcccaagatc cccatcgcgc cggccggcta gcgtgagagc
     3541 cctcgtcacc ctgctggtcc tgactctgct cgcgccggcg gcggctttcg cggagtcgcc
     3601 caagctcgac tgggagaccg gcgagggcaa gagctacgtg atcccggctc tcgagatcgg
     3661 cggcttcctc ctcgggctca accagttcaa ccggcacgtc atcgactcca aggagtacgg
     3721 gagcgacttc aagtcgatcg gcaacaatct cacgggcccg ctcgtcatcg accgggaccc
     3781 gttcagcgtc aaccagctgg gccatcccta ccagggcaac atgtacttcg gcttcgcgcg
     3841 gtcggccggc ctcgacttct ggcagtcgct ggcctacagc ctgggcggca gcctgctctg
     3901 ggagacggcg ggggagacca cgccgccctc cctgaacgat cacatcaaca ccgcgatcgg
     3961 cggcgcgttc atcggggagg ccgccttccg gatggccagc ctgctgctcg aaggcggcgg
     4021 ccagaacccc ggattctggc gcgagctcgg cgccgcggtg ctctcaccgc ccaccgggtt
     4081 caaccggctc gtcttcggcg accggttcga caaggtcttc ccgagccgca acccggaggt
     4141 cttcatccgg ctccgcctcg gggccacgct gaccaacgag gtgaacaacg acatcaagaa
     4201 ggacgtgaag cgccaggagg gcgcggcgga ctactcgatc acctacgggc ttcccggcaa
     4261 gcccggctac gagtacaagc gcccgttcga ctacttccac ttcgagttca ccgcggtccc
     4321 caacgcgggc gacatcaccg acgccatcga gaacgtgtcg atccgcgggc tcctcggcgg
     4381 gcgccgctac accctgggcg acgacttccg cggggtgtgg ggcgtcttcg gcaactacga
     4441 ctacttctcg ccgcagatct tccgggtgtc gagcaccgcg gtgtcgatgg gcaccgtctt
     4501 ccagtggtgg ctctcgcgcc acgtcgcgct ccagggcatc gcgctgggcg gcctcggcct
     4561 tggcgcggcg ggcaccgtcg gcgaccgcgc cgagcgcgag taccactacg gcctgacccc
     4621 gctgaccctg ctcggcatgc gactcatctt cggggaccgg gcgatgctgg aggcaacggg
     4681 ccgtcagtac tacgtggccg ggaccagctc caaccgggag accggggtgg acaccagcca
     4741 cttcagccag gacgtgatcc tgcgcggcag cttgggcttc acggtgcgtc tctgggggcc
     4801 gcacgccctc cgggtccagt acctcatctc gagccgcaac accggcgccc ccgacctgcg
     4861 cgatcggcac cagtcggtgc agaccgtcac catctcctac aacttcctcg gccacacgcg
     4921 cttcggcgcg gtggagtggc ggccggacca gtagaatcgg ggtatgctcg gccccacgct
     4981 cgcgcccacg ctcgcgaaga tcgtggcgtt cggcctcttc ctggcgctgg gcttcgcggc
     5041 gattcctctc atgctgcgcg tcttcctctg ggggcagcgc cggatcggca acgaggcggt
     5101 cccgatggtg cgcttcctcg ccgatcatca gaccggcgtg gtggtcgcgg tctggatcgt
     5161 cttcgcgctc gggctcggga tcgccctgcc cgccatgctg gccgacgagt tcttcgggcg
     5221 gcccgccaag ctctggttcg aggccaggct gcgcggcgcg agccggggcg tgctgaccgc
     5281 caacgtcggc atgaccctcg aggacattcg ccgccggtcg acgctcccga tgtccgagcc
     5341 ccgcggcgag tcgctgaccg gctcgagccg cctggccgcc gacgtggtct tcgatctcga
     5401 catcgccgac accggcacgc tcttcccggg g
//
LOCUS       DATMCV010000748         2366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_239084, whole genome shotgun sequence.
ACCESSION   DATMCV010000748 DATMCV010000000
VERSION     DATMCV010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239084"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VNU03_17150"
     CDS             complement(<1..473)
                     /locus_tag="VNU03_17150"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HWP76253.1"
                     /translation="MSPRALLLVLLAALCHSAWNLIVKTDARRLEIQSGALVVGTLMC
                     APVLFFYSPWTLSPHAWALIAVSALFESAYVLALTSAYGAGDMSLVYPVARGSGTVLV
                     APLAVLLLGERLSPQGVIGIGLVVAGIFLSHGLGRGALRGWAVAGAHRRALGWALL"
     gene            534..1589
                     /gene="mltG"
                     /locus_tag="VNU03_17155"
     CDS             534..1589
                     /gene="mltG"
                     /locus_tag="VNU03_17155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWP76254.1"
                     /translation="MMERPRSLKPWGDSPKHSGPTWRTAIGLLVLVAVLSLLLEGWWI
                     LSVPETLRATSRVVEVPAHKGVVEVAEILDDAGVIRSRVGFILLSVARGSFRSLKAGE
                     YQIPQGTNTVRVLERLEGGQVLQHMVVVQEGSTLAELAKQLETERLAPAADIMRVARD
                     AVFLKTLDVQADSVEGYLFPDTYQFVKGMTAEEILARMVARMREKVSPEIVAEARARD
                     LSVHQMLTLASIIEKEAVEPTEMPLISAVFWNRIRKDMPLQADPTVQYAVGKDRRRLT
                     REDLQTDSPYNTYRRQGLPPGPIASPGRAAIQAAVRPAHVNYLYFVAMDDRHHQFSTN
                     LADHNAAVARYRLARSR"
     gene            2021..>2366
                     /locus_tag="VNU03_17160"
     CDS             2021..>2366
                     /locus_tag="VNU03_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76255.1"
                     /translation="MTAMADERLISGCVGADRPEAQPYIPEVDGVLYSTCPERRKALE
                     ELELPQREWDSSRPDDTSPLPESRPLTQGLEENNMKHSRTMIANFVAFTLVLALSAVP
                     FATAENLANPSVN"
BASE COUNT          405 a          817 c          787 g          357 t
ORIGIN      
        1 agcagcgccc agccgagggc ccgccggtgg gctcccgcga cggcccagcc gcggagggcc
       61 ccgcggccga gcccatggct cagaaagatg ccggccacca cgagcccgat gccgatcacg
      121 ccctgcggcg acagccgctc gcccagcagg agcaccgcga gaggggcgac gagcaccgtc
      181 ccgctgccgc gggccaccgg gtagacgagc gacatgtccc ccgccccgta ggcggacgtc
      241 agggcgagca cgtaggcgga ctcgaacagc gcggagaccg cgatcaacgc ccaggcgtgc
      301 ggcgatagcg tccacggcga gtagaagaag agcaccgggg cgcacatcag ggtaccgacg
      361 acgagggcgc ccgactggat ctccaggcga cgcgcgtcgg tcttcacgat caggttccac
      421 gcgctgtggc agagagcggc cagcagcacc agcagcaggg cccgcggcga cattccgcca
      481 gtctaccggc ggtttgactc attaaagaag cccggccaaa atgagggggt cccatgatgg
      541 agcgtccccg atccctcaag ccgtggggcg acagccccaa gcactccggg ccaacctggc
      601 gcaccgcgat cggcctgctc gtgctcgtcg ccgtgctctc gctgctgctc gagggctggt
      661 ggatcctgag cgtgcccgag accctgcgcg cgacgtcgcg ggtggtcgag gtcccggccc
      721 acaagggcgt ggtggaggtg gccgagatcc tggacgacgc gggcgtgatc cgcagccggg
      781 tcggcttcat cctcctctcg gtagcccgcg gcagcttccg cagcctgaag gcgggcgagt
      841 accagatccc gcagggcacc aacaccgtcc gcgtgctcga gcgtctcgag ggcggccagg
      901 tgctgcagca catggtggtg gtccaggagg gcagcacgct cgccgagctg gccaagcagc
      961 tcgagacgga gcggctcgcg ccggccgccg acatcatgcg ggtggcccgt gacgcggtct
     1021 tcctcaagac gctggacgtc caggccgatt cggtcgaggg ctatctcttc cccgacacct
     1081 accagttcgt gaagggcatg accgccgagg agatcctggc ccgcatggtc gcgcgcatgc
     1141 gcgagaaggt gtcccccgag atcgtggccg aggcgcgggc ccgtgatctg agcgtgcacc
     1201 agatgctgac gctggcctcg atcatcgaga aggaagcggt ggagccgacc gagatgccgc
     1261 tcatctccgc ggtgttctgg aatcgcatcc gcaaggacat gccgctgcag gcggacccga
     1321 ccgtccagta cgcggtgggc aaggaccgcc ggcgcctcac ccgcgaggat ctccagaccg
     1381 actcgcccta caacacctat cgccgccagg ggctgccgcc cgggcccatc gcgagccccg
     1441 gacgcgcggc catccaggcc gcggtgcgac ccgcccacgt gaactacctc tacttcgtgg
     1501 cgatggacga ccggcatcat cagttctcga cgaacctggc cgatcacaac gccgccgtgg
     1561 cgcggtatcg gctggctcgg tctcgctagc ttctgtcgcg cggccatccg agggcgggcg
     1621 gggcgggagg ctggcggcga agccctcgtg ccaggtcagt agcgccttga gggcttcacc
     1681 aacctccact ttttcagggt gcctgatcct gttccctcgc tgtgctcggg tgtgccggcg
     1741 gacaccacgc ttgaccttcc cgactcgacg gcgcgtgaga gccgtgaccg gttacgaacc
     1801 gccgcaaggt gggcctcgag ttcccggtcg accgggtcac ggtcaatctc gcctccgcta
     1861 agcttcgcaa cgagggcgcc gccttcggcc tgccgggcgg cgggcatcct ctgcgccacg
     1921 taatccactt ccggcgtttc ccttcgaggg cctacgccag tggggttgcc agtgggttaa
     1981 ggggattccg gctccgcttt tgcaccgttg gatgtcactg atgaccgcga tggcagacga
     2041 gcgactcatc tccggctgtg tcggcgccga ccgccctgag gcccagccct acatccccga
     2101 agtcgacggg gtgctgtact ccacctgccc ggagcgccgg aaggcgctgg aggaactcga
     2161 gcttccgcag agggaatggg actcctctcg gccggatgac acatccccac ttcccgaatc
     2221 tagaccacta actcaaggac tagaagagaa caatatgaaa catagtcgga caatgatcgc
     2281 taacttcgtg gcattcacgc tcgtcctggc attgtcagcg gttccattcg caactgctga
     2341 gaacttggca aacccttcgg tgaaca
//
LOCUS       DATMCV010000749         9700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_37763, whole genome shotgun sequence.
ACCESSION   DATMCV010000749 DATMCV010000000
VERSION     DATMCV010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37763"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VNU03_17165"
     CDS             complement(<1..797)
                     /locus_tag="VNU03_17165"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76256.1"
                     /translation="MAAVAGSLLAAPRAGRAQEASKTYTVGLVSIGTDPGQSTLWQSF
                     IDGMRERGYVEGRNLVVKRAFGNPDRLSGMLAELVAARVDAIVTTGPRETRTAQRATA
                     TIPIVMTFVEDPVAEGFVKSLAQPGTNVTGLTSLVPGLSQKYVELLSEALPTARRFGV
                     VATRDPVPESRHELETAVKARGLTMSLLVVSEPVDFDTTLARAKRDGVTGIIAPVDGV
                     TLLHRRALVEAALKHRLPGIYWAREYVEAGGLMTYGASLADRRRHAA"
     gene            928..2169
                     /locus_tag="VNU03_17170"
     CDS             928..2169
                     /locus_tag="VNU03_17170"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP76257.1"
                     /translation="MPDAVPSELQSRVLDRIDDEEIVRLASDLIRIPSFTTEETPCAE
                     WLADYLGAQGLAVTLQEIEPGRKQTIARLAGTGVGRSIMLNGHIDIDPLASGWTRDPF
                     VPWIADGKLFGHGVFNMKAGVTACIMATLALRRAGVPLRGDVVLACVAAELNSGIGTI
                     HALEHGQRTDMAVVAEPYGARTILTKHTGTMDVVVHTLGRATHISKKEQGVDAIALMM
                     RAIQALEAMRFTHVHDPDLPGLPRLNVGSIIGGRGREHNLRGAYTVSDFCTAYVNVRF
                     NASQTGESVVADIRRTLDALAADDPRFRYEIEFPPALERGLGRLAKAAVDVPKTEAVV
                     QMVRRNLHRMTGAEPEHFGVRLPQSYAGNDTTHLWAAGIPCCIYGPGGGYTDYHDVHV
                     ELADLFLCTRVLALTALEAAL"
     gene            2172..3302
                     /locus_tag="VNU03_17175"
     CDS             2172..3302
                     /locus_tag="VNU03_17175"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWP76258.1"
                     /translation="MHYLDPSTGRSYPLDEPRWRADNGHYLNLAPGPGLTRRDIEPGR
                     RSVWRYARALAVGVEHAVSLGEGWTPLVDGRWHDAPVRYKLEFMMPTGSFKDRGMTVM
                     VSYLRSRGVTRVLEDSSGNAGASLSAYAAAAGLTCRILVPETASYPKIAQIAAGGAEV
                     VTIKGSRQDVAEAALRQSAEMFYASHNWQPFFVEGTKTLAYELWEQSGFRAPDNVVVP
                     LGYGSNVLGADRGFAELSRAGEIARRPRLFGVQAAHCAPYEAAFRAGVETLGPVEVRP
                     TVAEGIATPRPTRGPEVLRAVRESGGSVVAVEEAEIVQALRDLARRGLYVEPTAAAAA
                     AGLTRLLASGVIRPQEDTVLVLTGSGLKASATIGELLKLAPK"
     gene            complement(3377..3880)
                     /locus_tag="VNU03_17180"
     CDS             complement(3377..3880)
                     /locus_tag="VNU03_17180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWP76259.1"
                     /translation="MDANVKKTVLRMIPYGLYVLTARGKDDAVAAATVNWVTQASFAP
                     PLVVVGVKTDSHAHALIKESKAFALNVLGQGQQAMAFTFFKPATREGQTISGEPFRWG
                     STGAPILERTPAFVECRLVDTVERGDHSVFVGEVVEAGQAKPIEGRADDATLWLKALG
                     PNVFYGG"
     gene            4006..4759
                     /locus_tag="VNU03_17185"
                     /pseudo
     CDS             4006..4759
                     /locus_tag="VNU03_17185"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
     gene            4859..5575
                     /locus_tag="VNU03_17190"
     CDS             4859..5575
                     /locus_tag="VNU03_17190"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HWP76260.1"
                     /translation="MGADYGALLNVQTSARMLSQLEFFCGLAMKVADAIGITGFLHVQ
                     EKLGEMLSQMEIARAVFYGAEAMAQRLPSGVWVPGGHGLRAFHLQTGKVYSRFVEIVQ
                     TLAAGGFFYAPSEADLQSPELRPYIDRYVRGRAGVSAEERVALFKLAWDATGESFAQR
                     MAQYVRYYSGDPIRLTAGFYAQYDKAPLLDLVERALGHRDGVPLPITPVEPGSPLPYL
                     PDTRGMAGTYAATSLPPRQA"
     gene            5739..6062
                     /locus_tag="VNU03_17195"
     CDS             5739..6062
                     /locus_tag="VNU03_17195"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWP76261.1"
                     /translation="MNTPKHHRSRRRQPRAGVSWTVWIKAGPHRVRHHTVDISAHGAK
                     LRPRTQLQPGTPVQLHMVPPEGRALQISGVVWRVDSDGFVVLFLASLPAQHLTGERTA
                     ASVTH"
     gene            6172..7371
                     /locus_tag="VNU03_17200"
     CDS             6172..7371
                     /locus_tag="VNU03_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76262.1"
                     /translation="MAFTESQVMLTLAGLAYRGFHDLLHGRSHTDSVQRAVLDGLHTL
                     APVRGEWTLVWGPVTSRSGPDLFDAAAMYVVQSRREPTRYVVAIRGTNPIAASDWLLG
                     DFWVGTTVPWPYAPAADAVALSASAALGLSKLQALRSPRIALEPAADADARPVDPSVL
                     ADQIAGIDASWQRLASEPTMWLRDQVERLKRATALRWAILRPRSQPAPGDDAALDLLG
                     FLGDEARAAEGPLDVAVTGHSKGGALAPVVALWLQELLVSPDAAERWDGERGARVSCH
                     AFAGPTPGNARLADRIDRVLGAGHHHLRNANDVITHAWQIDELEAIPSLYAPRGATLE
                     RLIRLIVEKTRALDYRQTQVGVTTFAGPLDASRSFASELVHQHLEAYLAELRLLEAGV
                     RPLALFF"
     gene            complement(7385..8122)
                     /locus_tag="VNU03_17205"
     CDS             complement(7385..8122)
                     /locus_tag="VNU03_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWP76263.1"
                     /translation="MTTLQRAEAAEHALSQELDRVVVKSVLFTSGERDPRQPVSRPPD
                     NGRLYMMGHDSRLPRMPDRPTLFDFFRLRFGPSAHLLQSARLAMKNGVDEKIVLACLL
                     HDIAIAGFIRSDHGYWSAQLLEPYVDEEVAWGIRYHQALRFFPDESVGYRYPELYLKL
                     FGADYVPEPHIQEAYRYARDHKWYMTSRLITVNDLYAFDPSVQVELEEFTDVVGRHFR
                     QPKEGLGFDQSPSAHMWRTINWPTRYL"
     gene            8198..8629
                     /locus_tag="VNU03_17210"
     CDS             8198..8629
                     /locus_tag="VNU03_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76264.1"
                     /translation="MRSRIIPAAVVTIALLAASALPAPAQTTSKDVSQKASETGEAIK
                     DYTIEKKNEAVAQAKKLTADLDAKIKDLEAEVSKQTGEAKAKAQAQLRDLKAKRAAAS
                     RKASDLGKATKASWERAKEGFADAYRDLATAYEKAAAEFKK"
     gene            complement(8619..>9700)
                     /locus_tag="VNU03_17215"
     CDS             complement(8619..>9700)
                     /locus_tag="VNU03_17215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP76265.1"
                     /translation="LATHGQAALPAIMAGQHYTAAMRLVVSCVWTLCLLALVILWWRR
                     PHSVLDVWLMVVMSAWVFDIALAAVLNAGRFDLGFYAGRIYGLLAASFVLGVLLIEHG
                     RLYARLVTAHAGERRERQLVQLRTAELTAVNQDLEAFSYSVSHDLRAPLRAVRGYVTI
                     LEEDFGARLDGEPRRLLGVIQDRASRMEQLIEDLLAFARLGRQRLALTSVPLDDLVRE
                     SVEDLRPACDGREVTFNVEPLGRVEGDPALLKQVLVNLLGNAVKFTRTRHPAVVEVGR
                     RAETVGAAAYYVRDNGVGFDRARAEKLFGVFQRFHSPAEFEGTGVGLAIVQRVVERHG
                     GRVWAESAPGEGATFYFTLPGAATS"
BASE COUNT         1456 a         3582 c         3303 g         1359 t
ORIGIN      
        1 atcgccgcgt gtcgccgccg atcggcgagg ctggcgccgt aggtcatgag accgccggcc
       61 tctacgtact cccgggccca gtagatcccg ggcaggcgat gcttcaacgc cgcctccacg
      121 agcgcccggc gatgcaacaa cgtgacgcca tcgacggggg cgatgatgcc ggtgaccccg
      181 tctcgcttcg cccgagccag cgtcgtgtcg aaatccaccg gctccgaaac gacgagaagc
      241 gacatggtca acccacgcgc cttcaccgcg gtctccagct cgtgccggct ctcgggcacc
      301 ggatcgcgcg tcgcgacgac cccgaatcgc cgcgccgtcg gcagcgcctc gctgagcagc
      361 tccacgtact tctggctcag tcccgggacg aggctcgtga ggccggtcac attggttccc
      421 ggctgggcca ggctcttcac gaatccctcc gcgaccggat cctccacgaa cgtcatgacg
      481 atcggtatgg tggcggtcgc ccgctgggcc gtgcgagtct ctcgtggtcc cgtcgtcacg
      541 atggcgtcga cccgggccgc gaccagttcg gcgagcatac cggagagacg atccgggttg
      601 ccgaaggccc gcttgacgac gaggttccga ccctcgacgt agccccgctc acgcatgcca
      661 tcgatgaagg actgccagag ggtcgactgc ccaggatcgg tcccgatcga caccagtcca
      721 accgtgtacg tcttgctcgc ctcctgcgct cgtccggcgc gcggagcggc caggaggctg
      781 cccgcgacgg ccgccatgaa gacacgtcga gacaggtcag gcgggggaag cggctcccgg
      841 gggactctcc tcatagctcg cccgcgagtc taccagcgtc ggcgtgcatg gagagcagcg
      901 gcgatgccag ttgtacaatg gccccccatg cccgacgcgg tgccgtccga gctccagagc
      961 cgggtcctcg accggatcga cgacgaagag atcgtccggc tcgcctccga cctgatccgc
     1021 atcccgtcgt tcaccaccga ggagacgccc tgcgcggagt ggctcgccga ctacctgggc
     1081 gcgcaggggc tcgcggtgac gctgcaggag atcgagccgg gccgcaagca gaccatcgcg
     1141 cggctcgccg gcaccggcgt cggccgctcc atcatgctga acggccacat cgacatcgac
     1201 ccgctcgcgt ccggctggac gcgcgatccc ttcgtcccct ggatcgcgga cggcaagctc
     1261 ttcggccacg gcgtcttcaa catgaaggcg ggcgtcaccg cctgcatcat ggccaccctg
     1321 gccctccggc gcgccggggt cccgctccgg ggcgacgtgg tgctggcctg cgtggccgcg
     1381 gagctgaaca gcgggatcgg caccatccac gcgctcgagc acgggcagcg caccgacatg
     1441 gcggtggtcg ccgagcccta cggggcacgc accatcctga ccaagcacac cggcaccatg
     1501 gacgtggtcg tccacaccct cgggcgggcc acccacatct ccaagaagga gcagggcgtg
     1561 gacgccatcg cgctcatgat gcgcgccatc caggcgctcg aggcgatgcg cttcacccac
     1621 gtccacgacc cggacctgcc tggcctgccg cgcctcaacg tgggctcgat catcggcggc
     1681 cgcggccgcg agcacaacct gcgcggcgcc tacacggtga gcgacttctg caccgcctac
     1741 gtgaacgtgc gcttcaacgc gagccagacc ggtgagagcg tggtggccga catccgccgc
     1801 accctcgacg cgctcgccgc cgacgacccg cgcttccgct acgagatcga gttcccgccc
     1861 gcgctcgagc gcggcctcgg gcgcctcgcc aaggccgcgg tggacgtgcc caagacggaa
     1921 gcggtggtgc agatggtgcg gcgcaacctc caccgcatga ccggggccga gcccgagcac
     1981 ttcggggtgc gactgccgca gagctacgcg ggcaacgaca ccacgcacct ctgggccgcc
     2041 ggcatcccct gctgcatcta cggcccgggc ggcggctaca ccgactacca cgacgtgcac
     2101 gtggagctgg cggatctctt cctctgcacg cgcgtcctcg ccctcaccgc gctcgaggcg
     2161 gcgctctgac cgtgcactac ctcgatccct ccaccggccg gtcctacccg ctcgacgagc
     2221 cgcgctggcg cgccgacaac ggccactacc tgaacctggc cccgggcccg gggctcaccc
     2281 gccgcgacat cgagcccggg cggcgctcgg tgtggcgcta cgcgcgggcg ctcgcggtgg
     2341 gcgtggagca cgcggtgagc ctcggcgagg gctggacccc gctcgtcgac gggcgatggc
     2401 acgacgcgcc ggtgcgctac aagctcgagt tcatgatgcc gaccggctcg ttcaaggacc
     2461 gcggcatgac cgtgatggtc agctatctcc ggagccgcgg cgtcacccgc gtgctggagg
     2521 actcctccgg caacgcgggc gcctcgctct ccgcctacgc ggcggccgcc ggcctcacct
     2581 gccggatcct cgtgccggag accgcgtcct acccgaagat cgcgcagatc gccgcgggcg
     2641 gcgccgaggt ggtgacgatc aagggctcgc ggcaggacgt ggcggaggcc gcgctccggc
     2701 agagcgccga gatgttctac gcgagccaca actggcagcc cttcttcgtg gagggcacca
     2761 agaccctcgc ctacgagctg tgggagcagt cgggcttccg cgcgcccgac aacgtggtgg
     2821 tgccgctcgg ctacggcagc aacgtgctgg gcgccgaccg cggcttcgcg gagctctccc
     2881 gcgcgggcga gatcgcgcgg cggccccgcc tcttcggggt gcaggccgcc cactgcgcgc
     2941 cctacgaggc cgccttccgc gcgggagtgg agaccctcgg cccggtcgag gtgcgaccca
     3001 cggtggccga gggcatcgcc acccccaggc cgacgcgcgg gcccgaggtg ctgcgcgcgg
     3061 tgcgcgagag cggcggcagc gtggtcgcgg tcgaggaggc ggagatcgtc caggcgctcc
     3121 gtgacctggc ccggcgcggc ctctacgtgg agccgaccgc ggccgccgcg gcggcgggcc
     3181 tcacccgcct cctcgcgagc ggggtcattc gcccgcagga ggacaccgtc ctcgtgctca
     3241 ccggctccgg cctcaaggcc tccgccacca tcggcgagct cctcaagctc gcccccaagt
     3301 aaccctctcc cctagtaacc ctcgcccctc cggggagagg gcaggtgagg ggcgagctgg
     3361 tgaggggcgc ggaagtctac cccccgtaga acacgttcgg ccccagcgcc ttgagccaga
     3421 gcgtcgcgtc gtccgcgcgg ccctcgatgg gcttggcctg gcccgcctcc accacctcgc
     3481 ccacgaacac cgagtgatca ccccgctcga cggtgtccac cagccggcac tccacgaaag
     3541 cgggagtccg ctcgaggatc ggcgcgccgg tcgagcccca gcgaaacggc tcgccggaga
     3601 tcgtctgtcc ctcccgggtc gccggcttga agaaggtgaa agccatcgcc tgctgcccct
     3661 gtcccagcac gttgagggcg aaggccttcg actccttgat cagggcgtgg gcgtgggaat
     3721 cggtcttcac ccccacgacg accagcggcg gagcgaacga ggcctgggtc acccagttca
     3781 cggtggccgc ggccaccgcg tcgtccttgc cccgggcggt caggacgtag agcccgtagg
     3841 gaatcatgcg cagcaccgtc ttcttcacgt tggcgtccat ggcgcgctcc gtcctcgttg
     3901 accttcgttc gctgcggagt ctctcgccgg aattctgcca ccgccgcgcg cgcctgtcga
     3961 cccgccggtt gacttcgcgg cgcgtttcgt gaaagggtgg gcgccatgcc ggcgcgcagt
     4021 ggacgcgagt acgtcgaatc gatccggaag caggccccga gcgtcttcct gggcggccgg
     4081 cgcatcaccg acgtgaccgc ggagccggtc ttccaggagc cgctccgcgc catcgccgag
     4141 cagtacgaca tgcagctcga cccggcctat cgcgacgtga tgacctatcc gtcgcccacc
     4201 accggccagc cggtctcctc ctcgttcctg gtgccgcgct cgcgcgagga gctggtgaag
     4261 aagcgccagc acttcaagct gcgcgccgac cacaacttcg gattcatggg gcgctcgccc
     4321 gacttcatga accagttcgt gaccggctgg catctcatgg ccgaccgttt cgcgcgcgcc
     4381 ggcgcgctac ggagacaacg ccacccgcta ctacgagcac gtgcgcgagc acgatctctt
     4441 cctcacccac atgctcatca acccgcaggt cgatcgcagc aagacctcgg ccgagcagga
     4501 ggatccctac ctccacctgg ggcgggtgcg ggagacgtcc gacggcatca tcgtgcgcgg
     4561 ggccaagatg ctcggcacca tggcgccgct caccgaggag gtcgcggtga tccccttcgg
     4621 cgggatcccg ccgggcgacg acgcctacgc gctcgccttc gccatccccg ccgcgactcc
     4681 gggcctcacc ttcatctgcc gcgagacggt gtcgccgctg ccgcgctccc gcttcgacca
     4741 cccgctctcg agccggttcg aggagatgga ctgcatcgcg gtcttcgagg acgtgctggt
     4801 gccgtgggac cgggtcatgg tggacggcag cccgggcagc gcccaggtga tcaacacgat
     4861 gggcgccgac tacggggcgc tcctgaacgt gcagaccagc gcgcgcatgc tgagccagct
     4921 cgagttcttc tgcggcctcg ccatgaaggt ggccgacgcc atcgggatca ccggcttcct
     4981 ccacgtgcag gagaagctcg gtgagatgct gagccagatg gagatcgccc gcgcggtctt
     5041 ctacggcgcg gaggcgatgg cccagcggct gccgagcggg gtgtgggtgc cgggcggcca
     5101 cgggctgcgc gccttccacc tgcagaccgg caaggtctac agccgcttcg tcgagatcgt
     5161 gcagaccctc gcggcgggcg gcttcttcta cgcgccgagc gaggcggatc tccagagccc
     5221 cgagctgcgg ccctacatcg accgctacgt acggggccgc gccggcgtct ccgcggagga
     5281 gcgcgtcgcc ctgttcaagc tggcgtggga cgccaccggg gagagcttcg cgcagcgcat
     5341 ggcccagtac gtgcgctact actcgggcga tccgatccgc ctcaccgccg gcttctacgc
     5401 gcagtacgac aaggcgccgc tgctggacct ggtcgagcga gcgctcggcc accgcgacgg
     5461 cgtcccgctg ccgatcaccc ccgtcgagcc gggctccccg ctcccgtacc tgccggacac
     5521 gcgcgggatg gccgggacat acgcggccac ctcgctgccg ccgcgccagg cctgagccgg
     5581 aagaaacgct acggagattt tgcaggaggt ttgtgcccgg ctccgatcct ggtagtcgcc
     5641 tgacgaagtt cgttggtccc cgcccgccgc ctcatctgta acgtcccgat ttggcgggcc
     5701 ctcgatgctg gcgcgcggtt tgctcgtcat gcggggtcat gaacactccg aagcaccatc
     5761 gatcgcgtcg tcgccagccc cgggccgggg tgtcgtggac ggtctggatc aaggccggcc
     5821 cgcaccgggt ccgccatcac accgtcgaca tcagcgccca cggcgccaag ctgcggcccc
     5881 ggacccagct ccagccgggc acgcccgtgc agctgcacat ggtgccgccc gagggccggg
     5941 cgctccagat ctcgggcgtc gtctggcgcg tggacagtga cggcttcgtc gtcctcttcc
     6001 tggcgagcct tcccgcccag cacctgaccg gagagcgcac ggcggccagc gtcacccact
     6061 gatccacgca ggcgcccagg agcccttact gggtgctcga tgagcgccga gccaccgagt
     6121 aaggacagct tcgggttgac acgccgcggg cccgcgcccg atggtgtccc catggcgttc
     6181 accgaaagcc aggtcatgct gaccctcgcg ggcctggctt atcggggatt ccacgacctc
     6241 ctgcacggca ggtcgcacac ggactcggtg cagcgggccg tgctcgacgg cctccacacc
     6301 ttagcgccgg tccgcggcga gtggacgctc gtctggggcc cggtcaccag ccgcagcggc
     6361 cccgacctct tcgacgccgc ggccatgtac gtggtccaga gccggcggga gccgacccgg
     6421 tacgtggtgg ccatccgcgg cacgaacccg atcgccgcct cggactggct cctgggcgat
     6481 ttctgggtcg gcacgacggt gccgtggccg tacgcgcccg cggcggacgc cgtcgcgctc
     6541 tccgcgagcg ccgcgctcgg cctctccaag ctgcaggcgc tgcgctcgcc gcggatcgcg
     6601 ctcgagcccg ccgccgacgc ggacgcccgc cccgtggacc cgagcgtgct ggccgatcag
     6661 atcgcgggca tcgacgcctc gtggcaacgg ttggcgagcg agcccacgat gtggctccgc
     6721 gaccaggtcg agcgcctgaa gcgtgcgacc gcgcttcgat gggccatcct gcggccccgc
     6781 tcgcagcctg cccccggaga cgacgccgcg ctcgacctgc tgggcttcct gggcgacgag
     6841 gcccgcgcag ccgagggacc gctcgacgtc gcggttaccg gccacagcaa gggaggcgcg
     6901 ctggccccgg tggtcgcgct ctggctgcag gagctgctcg tctcaccgga cgcggcggag
     6961 cggtgggacg gggagcgcgg cgcccgcgtg tcctgtcacg ccttcgcggg cccgacgccc
     7021 ggcaacgcgc gcctggccga ccggatcgac cgggtcctgg gcgccggtca ccatcacctg
     7081 cgcaacgcga acgacgtgat cacccacgcg tggcagatcg acgagctgga ggcgatccca
     7141 tccctctacg cgccgcgggg agccacgctg gagcggctga tccggctcat cgtcgagaag
     7201 acccgcgcgc tcgactaccg gcagacccag gtgggggtga ccacgttcgc gggcccgctc
     7261 gacgcctccc gctcgttcgc gtcggagctc gtccatcagc acctggaggc ctacctggcc
     7321 gagctgcggc tgctcgaggc gggggtgcgc ccgctcgcgc tcttcttcta gagagagcgc
     7381 cgtcctacag gtagcgggtg ggccagttga tggtgcgcca catgtgcgcc gacgggctct
     7441 gatcgaagcc gagcccctcc ttcggctgcc ggaagtggcg ccccaccacg tcggtgaact
     7501 cttccagctc cacctgcacg ctgggatcga aggcgtagag gtcgttcacc gtgatgagcc
     7561 gcgaggtcat gtaccacttg tggtcgcgcg cgtaccgata cgcctcctgg atgtggggct
     7621 ccggcacgta gtccgccccg aagagcttca ggtagagctc cgggtaccgg tagccgaccg
     7681 actcgtccgg gaagaagcgc agcgcctggt ggtagcggat gccccacgcc acctcctcgt
     7741 cgacgtacgg ctcgagcagc tgggcgctcc agtagccgtg gtcggagcgg atgaagccgg
     7801 cgatcgcgat gtcgtggagc aggcaggcca ggacgatctt ctcgtcgacg ccgttcttca
     7861 tggcgagccg cgcgctctgc aggaggtgcg cagaggggcc gaagcgcagc cggaagaaat
     7921 cgaagagggt cggccggtcc ggcatccggg gcagccgcga gtcgtggccc atcatgtaca
     7981 ggcggccgtt gtccgggggc cgcgacaccg gctggcgggg atcccgctca ccggaggtga
     8041 acagcaccga cttcaccacc acccggtcga gctcctggct cagcgcgtgc tcggcagcct
     8101 cggcccgttg cagggtcgtc atgtgagtcc tcccgctcac gatcttcgta ctcgccggcg
     8161 tcgtctgtca aggtagactc tcccgcggga ggtgcccatg cgctcacgga tcatcccggc
     8221 cgccgtcgtc acgatcgcgc tgctcgccgc gtccgcgctc cccgccccgg cccagaccac
     8281 gtccaaggac gtctcccaga aggcgtcgga gaccggggag gcgatcaagg actacacgat
     8341 cgagaagaag aacgaggcgg tggcccaggc caagaagctc accgccgacc tcgacgccaa
     8401 gatcaaggat ctggaggcgg aagtctccaa gcagaccggg gaggccaagg cgaaggccca
     8461 ggcccagctc cgggacctca aggccaagcg ggccgcggcc tcccggaagg cgagcgacct
     8521 cggcaaggcc acgaaggcgt cgtgggagcg ggcgaaggag ggcttcgccg acgcttaccg
     8581 cgacctggcg accgcctacg agaaggcggc cgccgagttc aagaagtagc cgcccccggc
     8641 agggtgaagt agaacgtggc gccttccccg ggggcggact cggcccacac ccgcccgccg
     8701 tggcgctcga cgacccgctg cacgatcgcc agccccacgc ccgtcccctc gaactccgcg
     8761 gggctgtgga atcgttggaa caccccgaag agcttctcgg cccgcgcccg gtcgaagccg
     8821 accccgttgt cccgcacgta gtacgcggcc gcgcccaccg tctccgcccg gcggccgacc
     8881 tcgacgaccg cgggatggcg ggtccgggtg aacttgaccg cgttgccgag caagttcacc
     8941 aggacctgct tgagcagggc cgggtcgccc tcgacccgac cgagcggctc cacgttgaac
     9001 gtgacctcgc gcccgtcgca cgcggggcgg agatcctcga ccgactcccg caccagatcg
     9061 tcgagcggca ccgacgtgag cgccagccgc tggcgcccga ggcgggcgaa cgcgagcaga
     9121 tcctcgatga gctgctccat gcggctcgcc cggtcctgga tgactcccag gagccggcgc
     9181 ggctcaccgt cgagtcgcgc cccgaagtcc tcctccagga tcgtcacgta gccgcggacc
     9241 gcccggaggg gcgcgcggag atcgtgggag accgagtagg agaacgcctc caggtcctgg
     9301 ttcaccgcgg tcagctccgc ggtccggagc tggacgagct gccgctcgcg tcgctcgccc
     9361 gcgtgggccg tgaccaggcg ggcgtagagt cggccgtgct cgatcaggag caccccgagc
     9421 acgaagctgg ccgccagcag gccgtagatc cgcccggcgt agaagccgag atcgaagcgg
     9481 cccgcgttga ggacggcggc gagcgcgatg tcgaagaccc acgcgctcat caccaccatg
     9541 agccacacgt cgagcaccga gtggggccgc cgccaccaca ggatcacgag cgcgagcaag
     9601 cacagggtcc acacacacga caccacgagc cgcatcgccg cggtgtagtg ctggccggcc
     9661 atgatggcgg gcagcgcggc ctggccgtgc gtggccagga
//
LOCUS       DATMCV010000750         3447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_704594, whole genome shotgun sequence.
ACCESSION   DATMCV010000750 DATMCV010000000
VERSION     DATMCV010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704594"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..483
                     /locus_tag="VNU03_17220"
     CDS             58..483
                     /locus_tag="VNU03_17220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP76266.1"
                     /translation="MTVARHSQTFPARRDALPRVDAFLGEVCSGAGLGRDTCLRLTLL
                     VEELFTNTVVHGHGGDSEAPVRIECEVSPGRVALIYEDTGPAHDPFARVITPDTGAGV
                     EERPVGGLGVLLVSEMAQQVEYRRAGDRNRISLVIATRA"
     gene            562..2109
                     /locus_tag="VNU03_17225"
     CDS             562..2109
                     /locus_tag="VNU03_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWP76267.1"
                     /translation="MNWKSEMDELRRREEFAEQLGGAERVKRQHDGGRYTIRERIDRL
                     VDGGSFHEIGKIAGKASYDGKNDLKELTPSNFVFGRARLDGRPVVVGGDDFTVRGGSA
                     DATIKGKHNQCEQMANELRLPLVRLVEGSGGGGSVKTIETTGRANVPGVSGWEWVVNS
                     IGTVPRVALGLGSVAGLGAAHLAAAHLSVMVKDKSALFVAGPPVVERLGQKLTKNELG
                     GWQIQLKAGAVDLAVDTEEQAFEVTRRFLSYLPSSIYELPPRGPRTDDPERREEWLGD
                     AIPRDIRKPYKMRTIIEAVVDRDSFFEIQPLYGRAIITGLARLDGWPVAVLASDPYFY
                     GGAWTADTCQKVERFVDFAQTFHLPVFYLVDCPGFLIGLEAEQTGTIKQGVRAMSAIW
                     QSTVPWCAVIVRNVFGVAGAAHKPGARYGPRYAWPSGRWGSLPLEGGIEAAYRADIQG
                     AADPAAKMIEIEERLQKLRSPFRSAETFWIEEIIDPRATRPLLCEFAELAAPLRETGA
                     YTHRMRP"
     gene            2212..2985
                     /locus_tag="VNU03_17230"
     CDS             2212..2985
                     /locus_tag="VNU03_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76268.1"
                     /translation="MYQAFDLHGKTAIVTGGNSGIGLGMAEALAQAGAAVCIWGTNEE
                     KNHTAVARLHAIGGKAMALRCDVSDEAAVDRAFAETVSTLGGVDACFVNAGVSGRGAA
                     ASFAEMTIEEWHRVLAVNLDGAFFTLRAAARHMIGRGEGGSLAVTASLAALEGSPLSA
                     HYAASKGAMLSVMRALAVGLARHKIRCNAIVPGWIETPMTEKAVHGDAFKTRVLPRVP
                     ARRWGTGGDFGGIAVYLTSDASNYHTGDTFVIDGGYAIF"
     gene            complement(3097..>3447)
                     /locus_tag="VNU03_17235"
     CDS             complement(3097..>3447)
                     /locus_tag="VNU03_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWP76269.1"
                     /translation="DPRHHVEKVYWVQVERVPDEDALARLRAGLVLKDGPTRPARARL
                     IEEPPGLWAREPPIRWRANIPTAWVEIGLTEGRNRQVRRMTAAVGFPALRLIRVAVGA
                     YGLGGLRPGEWREG"
BASE COUNT          501 a         1241 c         1224 g          481 t
ORIGIN      
        1 cgcgcgcggg ccgttcccat gcctcggata taccaccgcc ggccgtacaa taggccgatg
       61 acggttgcgc gccacagcca gacgttcccc gcccgtcgcg acgcgttgcc acgggtcgac
      121 gccttcctcg gcgaggtttg ctccggggcc gggctcgggc gtgacacctg cctgcggctc
      181 accctcctgg tggaggagct cttcaccaac accgtggtgc acggccacgg gggggacagc
      241 gaggcgccgg tgcgtatcga gtgcgaggtg agtcccggcc gtgtcgcgct catctacgag
      301 gacaccggcc ccgcgcacga ccccttcgct cgggtgatca ctcccgacac gggcgcgggc
      361 gtcgaggaac gcccggtggg tgggctcggc gtgctgctcg tctcggagat ggcccagcag
      421 gtggaatacc ggcgggccgg cgaccggaac cgcatctcgc tggtcatcgc cactcgcgcc
      481 tgatcgccgc cgtccccgct ccggccgagc ctaggcaggg gcgggcgctc cctgctacca
      541 tcctccgcgg aggtctcccc catgaactgg aagtccgaga tggacgagct gaggcgccgc
      601 gaggagttcg cggagcagct gggcggcgcc gagcgggtca agcggcagca cgacggcgga
      661 cgctacacga tccgcgagcg catcgaccgc ctcgtcgacg ggggcagctt ccacgagatc
      721 ggcaagatcg cgggcaaggc gtcgtacgac ggcaagaacg acctcaagga gctgacgccg
      781 tcgaacttcg tcttcggccg ggcgcggctc gacggccgcc cggtggtggt gggcggcgac
      841 gacttcacgg tccggggcgg gtcggccgac gcgaccatca agggcaagca caaccagtgc
      901 gagcagatgg ccaacgagct gcgcctgccc ctggtccgcc tggtcgaggg ctcgggcggc
      961 ggcgggtcgg tgaagacgat cgagaccacc ggacgggcca acgtccccgg cgtgtccggc
     1021 tgggagtggg tggtcaacag catcggcacc gtgccgcgcg tggcgctcgg cctgggctcg
     1081 gtggccgggc tcggtgccgc gcacctggcc gcggcgcatc tctcggtcat ggtgaaggac
     1141 aagtcggccc tcttcgtggc ggggccgccc gtcgtcgagc ggctcgggca gaagctcacc
     1201 aagaacgagc tgggcggctg gcagatccag ctcaaggccg gggcggtcga cctcgcggtg
     1261 gacaccgagg agcaggcgtt cgaggtgacc cggcgcttcc tctcctatct gccttcgtcg
     1321 atctacgagc tgcccccgcg cgggccgcgc accgacgacc ccgagcggcg cgaggagtgg
     1381 ctgggcgacg cgatcccgcg cgatatccgc aagccgtaca agatgcgcac gatcatcgag
     1441 gcggtggtgg accgcgattc gttcttcgag atccagccgc tctacggccg cgccatcatc
     1501 accgggctgg cccggctcga cggctggccg gtcgcggtgc tggcgagcga tccttacttc
     1561 tacggcgggg cgtggaccgc ggatacctgc cagaaggtgg agcgcttcgt cgacttcgcc
     1621 cagaccttcc acctgccggt cttctacctg gtggactgcc ccggctttct catcgggctc
     1681 gaggccgagc agaccggcac catcaagcag ggcgtgcgcg cgatgtcggc catctggcag
     1741 tccaccgtcc cgtggtgcgc ggtcatcgtc cgcaacgtgt tcggggtggc cggcgcggcc
     1801 cacaagccgg gcgcccgcta cggcccgcgc tacgcctggc cctcggggcg ctgggggtcg
     1861 ctgccgctcg agggcggcat cgaggccgcg taccgggccg atatccaggg cgcggccgat
     1921 ccggccgcga agatgatcga gatcgaggag cggctgcaga agctccgctc gccgttccgc
     1981 tccgcggaga cgttctggat cgaggagatc atcgacccgc gcgccacccg gccgctgctc
     2041 tgtgagttcg cggagctggc cgcgccgctg cgggagacgg gggcgtatac gcatcgcatg
     2101 cggccttgag aggtaccaac ctcccctcac cctcaaccac ccttaccctc acccgcccct
     2161 caccctgccc tctcccctga ggggagaggg atatgaggat gaaaggctct tatgtaccag
     2221 gcgttcgatc tgcacggcaa gaccgcgatc gtgaccggcg gcaacagcgg catcggcctc
     2281 gggatggcgg aggcgctcgc gcaggcgggc gcggcggtgt gcatctgggg caccaatgag
     2341 gagaagaacc acaccgcggt cgcccggctg cacgcgatcg gcggcaaggc gatggcgctg
     2401 cgctgcgacg tgagcgacga ggccgcggtg gaccgcgcct tcgccgagac cgtctcgacc
     2461 ctgggcggcg tcgacgcctg cttcgtcaac gcgggggtct cgggccgggg agccgcggcc
     2521 agcttcgccg agatgaccat cgaggagtgg caccgggtgc tggcggtcaa tctcgacggg
     2581 gccttcttca ccctccgcgc ggcggcacgg cacatgatcg ggcgcgggga gggcggctcg
     2641 ctcgcggtca ccgcgagcct ggccgcgctg gagggctcgc cgctcagcgc gcactacgcg
     2701 gccagcaagg gcgcgatgct ctcggtgatg cgggccctcg cggtcggcct cgcacgccac
     2761 aagatccggt gcaacgcgat cgtgccgggc tggatcgaga ccccgatgac cgagaaggcg
     2821 gtccacggcg acgcgttcaa gacccgcgtg ctcccgcggg tgcccgcgcg gcgctggggc
     2881 accggcggcg acttcggcgg catcgcggtc tacctcacca gcgacgccag caactaccac
     2941 accggcgaca ccttcgtcat cgacggcggc tacgccatat tctagagagc ctctcctcct
     3001 catacccctc tcccctcagg ggagagggca gggtgagggg cggtccaggg tgaggggtgg
     3061 tcaaggacga ggggctgtcc aggctgaggg gcgctctcaa ccctcccgcc actccccagg
     3121 ccgcaacccc ccgagcccgt acgccccgac cgcgacccga atcaaccgca gcgcgggaaa
     3181 ccccaccgcc gccgtcatcc gccgcacctg ccggttccgc ccctcggtga gcccgatctc
     3241 gacccacgcg gtcggaatgt tcgcccgcca ccggatcggc ggctcgcgcg cccacaggcc
     3301 gggcggctcc tcgatcagcc gcgcccgcgc cggccgtgtc gggccgtcct tcagcaccag
     3361 ccccgcacgc agccgcgcga gcgcgtcctc gtcgggcacg cgctccacct gcacccagta
     3421 gaccttctcc acgtggtggc gcggatc
//
LOCUS       DATMCV010000751         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_107020, whole genome shotgun sequence.
ACCESSION   DATMCV010000751 DATMCV010000000
VERSION     DATMCV010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107020"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VNU03_17240"
     CDS             complement(<1..997)
                     /locus_tag="VNU03_17240"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76270.1"
                     /translation="MATTRREFLKVAGAAATVGAVGFPAVARGQARPGVPSDPVKIGV
                     LAIRAGIAAPVGQSGLRGTEWWADRVNKAGGILGRPVQLVVEEESTPKDTVERYRKLI
                     LQDKVEVVIGGISTGVTLALGPVAEEMEVPWLSWDGTTQKGVEETMPNPKWAFRSVDN
                     EVEAIVAGQLTPKYFKGIKTVAGIGNDYSYGHDCWETYQAVLKRYIPDVKFVLELFPK
                     LGVTDFTSHIAAIQQAKPDLLMCSFWSGDATIIMKQAAAVGLFKSMKGVFTTAGGVHD
                     SLKKEFTPEGLLLGYNTMYFDDPKGSPLLKQFVREYKAKYNEYPAYECDHAYFNIE"
     gene            complement(1031..2065)
                     /locus_tag="VNU03_17245"
     CDS             complement(1031..2065)
                     /locus_tag="VNU03_17245"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWP76271.1"
                     /translation="MASSLPLLYTHVMGSHGFPSWFWTALDRIKAGEYGQTDVRETFD
                     DATQLAIRDQERAGIDVICDGEMRRYFFVQTFYGRMEGLETLEPLRKTGLYAYDSVPR
                     YRATRKVTVPKGLGTVDEFKYLVSQTDRPVKATCPGPVTLSIHIQTRPGDAYDGDRLA
                     LCWDLVPAVNAELRALAAAGATWIQVDEPSAAIVPGQAAEYVKMFNACVEGVKAKIGY
                     HVCFGNLLSRPRGKRSYRWMFPGLLETRCDQFVFEYANREMAEIEMWKEIGVDRDVAC
                     GVVDVKSFYMETPEDIAERVALCLESIPAERLSLIPDCGFFPVPRWVAFEKLKRLTAG
                     VALARKRLAG"
     gene            complement(2078..>2535)
                     /locus_tag="VNU03_17250"
     CDS             complement(2078..>2535)
                     /locus_tag="VNU03_17250"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWP76272.1"
                     /translation="IAAVQGHALGGGLMLAIAQDLRVAEASARFGLPEVTLGFNPGYG
                     IARLLDVAGGAHARDLMLTARPVDAAEAHRMGLVTRVVPDGTLDAAATTLAHDVARSP
                     RGGLAATKAITAALRAGRPGTEPQAYGALRAGPEAQARIAAFLDRRRSS"
BASE COUNT          356 a          877 c          893 g          409 t
ORIGIN      
        1 actcgatgtt gaagtaggcg tggtcgcact cgtaggcggg gtactcgttg tacttggcct
       61 tgtactcgcg cacgaactgc ttgagcagcg ggctgccctt gggatcgtcg aagtacatgg
      121 tgttgtagcc gagcagcagc ccctccgggg tgaactcctt cttgagcgag tcgtgcaccc
      181 cgcccgcggt ggtgaagacg cccttcatgc tcttgaagag accgaccgcg gcggcctgct
      241 tcatgatgat cgtcgcgtcg cccgaccaga acgagcacat cagcagatcc ggcttggcct
      301 gctggatcgc cgcgatgtgc gaggtgaagt cggtgacccc gagcttgggg aagagctcga
      361 gcacgaactt cacgtcggga atgtagcgct tgagcaccgc ctggtaggtc tcccagcagt
      421 cgtggccgta ggagtagtcg ttgccgatgc cggccaccgt cttgatgccc ttgaagtact
      481 tgggcgtgag ctggccggcc acgatggcct cgacctcgtt gtccacgctg cggaaggccc
      541 acttggggtt gggcatggtc tcctccacgc ccttctgggt ggtgccgtcc caggagagcc
      601 acggcacctc catctcctcg gcgaccggcc cgagcgcgag ggtcacgccg gtggagatgc
      661 cgccgatcac cacctcgacc ttgtcctgca ggatgagctt gcggtagcgc tccacggtgt
      721 ccttgggggt cgactcttcc tcgaccacca gctgcaccgg acggcccagg atgccgcccg
      781 ccttgttgac ccgatcggcc caccattcgg tgccgcgcag cccggactgc cccaccggcg
      841 ccgcgatgcc ggcccggatg gccaggaccc cgatcttgac cgggtccgac ggcactccgg
      901 gcctggcctg cccgcgggct actgcgggga agcccaccgc gccgaccgtc gcggcggcgc
      961 cggccacctt caggaactcg cgtctcgtcg tcgccatctc gttctccctc tcgtcgttga
     1021 gagtcgtcgg ttagccggcc agccgcttgc gcgcgagcgc cacgcccgcc gtgaggcgct
     1081 tgagcttctc gaaggcgacc cagcgcggca ccgggaagaa gccgcagtcc gggatcagcg
     1141 agagccgctc ggccgggatg gactcgaggc agagcgccac gcgctccgcg atgtcctcgg
     1201 gagtctccat gtagaagctc ttcacgtcaa ccaccccgca cgcgacgtcg cggtccaccc
     1261 cgatctcctt ccacatctcg atctcggcca tctcccggtt ggcgtactcg aagacgaact
     1321 ggtcgcaccg ggtctcgagc agccctggga acatccaccg ataggatcgc ttgccccgcg
     1381 ggcgcgacag gaggttgccg aagcagacat ggtagccgat cttggccttg accccttcca
     1441 cgcaggcgtt gaacatcttc acgtactcgg cggcctggcc gggcacgatg gcggcggagg
     1501 gctcgtcgac ctggatccag gtggctcccg ccgcggccag cgcccggagc tccgcgttca
     1561 ccgcgggcac caggtcccag cagagcgcga gccggtcgcc gtcgtaggcg tcgcccggcc
     1621 gggtctggat gtggatcgag agcgtcaccg ggcccgggca ggtggccttc accggtcggt
     1681 cggtctggct caccaggtac ttgaactcgt ccaccgtgcc gaggcccttg ggcaccgtga
     1741 ccttccgggt ggcgcggtag cgcggcacgc tgtcgtaggc gtagaggccg gtcttgcgca
     1801 gcggctcgag ggtctcgaga ccctccatgc ggccgtagaa ggtctgcacg aagaagtagc
     1861 ggcgcatctc gccgtcgcag atcacgtcga tgccggcgcg ctcctggtcg cggatcgcga
     1921 gctgggtggc gtcgtcgaag gtctcccgga cgtcggtctg cccgtactcg cccgccttga
     1981 tccggtcgag cgcggtccag aaccaggagg ggaagccgtg gctgcccatg acgtgggtgt
     2041 agaggagcgg cagcgaggag gccatgacgg ttcctttcta cgatgagcgg cgtcgatcga
     2101 ggaaggcggc gatgcgcgcc tgcgcctccg ggcccgcgcg cagggcgccg taggcctgcg
     2161 gctcggtgcc gggccggccg gcccggagcg cggcggtgat ggccttcgtg gccgccaggc
     2221 cgccgcgcgg cgagcgcgcc acgtcgtggg ccagggtcgt ggccgcggcg tcgagggtgc
     2281 cgtccggcac cacgcgggtc accaggccca tgcggtgggc ctcggccgcg tccaccgggc
     2341 gcgcggtgag catcaggtcc cgggcgtggg cgccgcccgc gacgtcgagc agccgcgcga
     2401 tcccgtagcc cgggttgaag ccgagggtga cctcgggcag gccgaagcgc gccgaggcct
     2461 ccgcgacccg caggtcctgg gcgatggcga gcatgagccc gccgcccagg gcgtggccct
     2521 gcaccgccgc gatcg
//
LOCUS       DATMCV010000752         2861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_574747, whole genome shotgun sequence.
ACCESSION   DATMCV010000752 DATMCV010000000
VERSION     DATMCV010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574747"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VNU03_17255"
     CDS             <1..380
                     /locus_tag="VNU03_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76273.1"
                     /translation="DNARYRDYILASRGEFTVAKDQNVRLRSGWFSDRSACYLAAGRP
                     VVTQDTAFGKFVPTGEGLFAFTSLDDILAALEAINSDYARHARAAREIAEAYFKAETV
                     LARLVADAGVSPSPRNPGMMRQP"
     gene            377..1621
                     /locus_tag="VNU03_17260"
     CDS             377..1621
                     /locus_tag="VNU03_17260"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76274.1"
                     /translation="MTRVLTGATLIDGTGAAPVPDAAVVIDGDRITAAGPRAAVTWPA
                     DAEVIDARGRTVIPGLIDAHDHLASHGYGLATRFGLEEPASTAHLRTARVLAETLAMG
                     YTTVRDAGGLDAGFKLAVEQGLIPGPRLVLALQIISPTGGIGDRVSPSGHDCCGAYDP
                     LLPPCVANGSDAVRDVVRTMVRAGADVIKTATTGGASSRPGHGPLDAAFSLAEMEALV
                     TESHALGRRVMCHALGGPGLRTALVAGVDSIEHGCYLDEDGTLLGQMAVQGTFFVPTL
                     TVYVYHRESPAPHVRARAVALHAHHVASVRRALELGVPIAAGTDAGGHGHPKNALELK
                     YLVEAGLSPMQALRAGTQWAAACLGLEREIGTIERGRLADLVLVGGNPLDDVTLLLDP
                     ARIELVLKGGVICADRRGPRPH"
     gene            1717..>2861
                     /locus_tag="VNU03_17265"
     CDS             1717..>2861
                     /locus_tag="VNU03_17265"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76275.1"
                     /translation="MRKPTAVRSLLLGLAALLAAPLAASAQEEIRLGAFLPISGISAD
                     VGAQMKAGTEVALERMATVQVGGKPAKVRVIWYDDEGKGDVGLNVVTRALTVDKIHVG
                     MGFLSSDVFIRVMDEFQKASTPVVTCCSASLKIGDKIAQAKMEYAFQLSPTANDIARS
                     VAAAVAMHVKPKKIALLNENTDAGRDFSRIVRDWFGANAKDVEVAADEFVDRGVTDLT
                     PQWAKIKRTGAQATIGEIYGSSGPVLFNQWFELKVPSVVAHMGATVSAQTFVDQHVKQ
                     MDLAIVNNRWWPAKYSAVSEPMMAAYKKKTGTDPTNFAVQAHDATLVLLDAIQKAGSL
                     DPQKIQAALESTSFVTAWGTRKFTPIAEGHRMPIQTVVVQIQSGKKVP"
BASE COUNT          424 a         1039 c         1015 g          383 t
ORIGIN      
        1 acgacaacgc gcgctatcgc gactacatcc tcgcctcgcg cggggagttc acggtggcca
       61 aggaccagaa cgtccgcctg cggagcggct ggttcagtga tcggagcgcc tgctatctcg
      121 ccgcaggccg gccggtggtc acccaggaca ccgccttcgg gaagttcgtc cccaccggcg
      181 aggggctgtt cgcgttcacg tccctcgacg acatcctcgc cgcgctcgag gccatcaact
      241 ccgactacgc gcgacacgcc cgggcggccc gcgagatcgc cgaggcctac ttcaaggccg
      301 agacggtgct ggcgaggctg gtcgcggatg ccggagtgag cccctcgccg cgaaatccag
      361 gtatgatgcg ccagccatga cccgcgtcct gaccggcgcc accctgatcg acgggaccgg
      421 cgcggctccc gtcccggacg cggcggtggt catcgacggc gaccggatca ccgcggccgg
      481 cccgcgcgcc gcggtcacct ggcccgccga cgcggaggtc atcgacgccc gcggccgcac
      541 cgtcattccc ggcctgatcg acgcccacga tcatctcgcc tcccacggct acggcctcgc
      601 cacgcgcttc ggcctggagg agccggccag caccgcgcac ctgcgcaccg cgcgcgtgct
      661 cgccgagacg ctcgcgatgg gctacaccac cgtgcgcgac gcgggcggcc tggacgcggg
      721 gttcaagctg gccgtcgagc aggggctgat cccggggccg cgcctggtgc tggcgctgca
      781 gatcatctcg ccgaccggcg gcatcggcga ccgggtgagc ccgtcgggcc acgactgctg
      841 cggggcctac gacccgctgc tgccgccctg cgtggccaac gggtcggacg cggtgcgcga
      901 cgtggtccgc acgatggtgc gggccggcgc cgacgtgatc aagaccgcga ccaccggggg
      961 cgccagctcg cgcccggggc acgggccgct cgacgcggcc ttctcgctcg cggagatgga
     1021 ggcgctggtg accgagtcgc acgccctcgg ccgccgggtg atgtgtcacg cgctcggcgg
     1081 ccccgggctg cgcaccgcgc tggtcgcggg cgtcgactcg atcgagcacg ggtgctatct
     1141 cgacgaggac gggaccctgc tcgggcagat ggcggtccag ggcaccttct tcgtgccgac
     1201 cctgacggtg tacgtctacc accgcgagag cccggccccg cacgtgcggg cccgcgcggt
     1261 cgccctccac gcgcaccacg tggcgagcgt gcggcgcgcg ctcgagctgg gcgtgccgat
     1321 cgcggcgggc accgacgcgg gcgggcacgg acacccgaag aacgcgctcg agctcaagta
     1381 cctggtcgag gccgggctca gcccgatgca ggcgctccgc gcgggcaccc agtgggccgc
     1441 cgcgtgcctc gggctcgagc gcgagatcgg caccatcgag cggggccgcc tggccgatct
     1501 ggtgctggtc gggggcaatc ccctcgacga cgtcacgctg ctgctggatc ccgcgcgcat
     1561 cgagctggtg ctgaagggcg gcgtgatctg cgcggaccgg cgcggcccgc gtccccacta
     1621 gccgcgggcg gagcgcgcgt ccgggcctcg tccgcgcgtg tgctatcatc ccgccaccgt
     1681 gcgtcccccc acgatgcccc acgcggaggc gtgatcatgc gcaagcccac ggcggtccgg
     1741 tcgctcctgc tcggcctcgc cgcgctcctc gcggctcccc tcgccgcctc cgcgcaggag
     1801 gagatccggc tgggcgcgtt cctgcccatc tccgggatca gcgccgacgt cggggcgcag
     1861 atgaaggcgg gcacggaggt cgcgctcgag cggatggcca ccgtccaggt gggcggcaag
     1921 cccgcgaagg tccgcgtcat ctggtacgac gacgagggca agggcgacgt cgggctcaac
     1981 gtggtgacac gcgcgctcac ggtggacaag atccacgtgg ggatgggctt tctctccagc
     2041 gacgtgttca tccgggtgat ggacgagttc cagaaggcgt cgaccccggt ggttacctgc
     2101 tgctcggcgt cgctcaagat cggcgacaag atcgcccagg ccaagatgga gtacgcgttc
     2161 cagctgagtc ccaccgccaa cgacatcgcg cgctcggtgg cagcggcggt ggcgatgcac
     2221 gtgaagccga agaagatcgc gctgctgaac gagaacaccg acgcggggcg cgacttctcg
     2281 cgcatcgtgc gcgactggtt cggggccaac gccaaggacg tggaggtcgc ggccgacgag
     2341 ttcgtggacc gcggggtgac cgacctgacc ccgcagtggg ccaagatcaa gcgcaccggg
     2401 gcccaggcca ccatcggcga gatctacggc tcgagcgggc cggtcctctt caaccagtgg
     2461 ttcgagctca aggtgccgtc ggtggtggcc cacatgggcg ccaccgtgag cgcgcagacc
     2521 ttcgtcgacc agcacgtcaa gcagatggat ctcgccatcg tcaacaaccg ctggtggccc
     2581 gccaagtact ccgcggtgtc cgagccgatg atggcggcct acaagaagaa gaccgggacg
     2641 gatcccacca acttcgcggt gcaggcgcac gacgccaccc tggtcctgct cgacgccatc
     2701 cagaaggcgg gcagcctcga tccgcagaag atccaggccg cgctcgagag cacctcgttc
     2761 gtgaccgcgt ggggcacccg caagttcacc ccgatcgccg aggggcaccg catgcccatc
     2821 cagaccgtgg tggtgcagat ccagtccggc aagaaggtgc c
//
LOCUS       DATMCV010000753         4784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_507151, whole genome shotgun sequence.
ACCESSION   DATMCV010000753 DATMCV010000000
VERSION     DATMCV010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507151"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VNU03_17270"
     CDS             <1..215
                     /locus_tag="VNU03_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76276.1"
                     /translation="AGLNSSLDPEVVCAQFGWWQACPPLGLPGYDADGPGSANYNALI
                     DPEATDPISGTVALRSYLCEVRRGDP"
     gene            212..976
                     /locus_tag="VNU03_17275"
     CDS             212..976
                     /locus_tag="VNU03_17275"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWP76277.1"
                     /translation="MSIDQAIDAFWQCARQRTAPGPEWMKRLSREEAYRVQLGVLARY
                     VAAGERQAGWKVGLTSKAMQAQQGVHEPVLGFLLQSGEQPSGTRFRYADLIRPGFENE
                     LCLTVGTTLRGPGVTTERAAAALAAMAPAFEIVERRGDFAADLNVSLADNAQQRAYVT
                     GPPVPVPPGFDLATVVVELSVNGAAAERALGSEVLGTPAASIAWLANKLAELGLALEA
                     GMKVMSGSFTRQYLLNAGDRIEASFTPVGQVRAEFI"
     gene            complement(989..1951)
                     /locus_tag="VNU03_17280"
     CDS             complement(989..1951)
                     /locus_tag="VNU03_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HWP76278.1"
                     /translation="MKAIRVSEYGGPSVLKLEEIPTPQPGPGQVLVRHHAVGINPVDT
                     YLRSNTDNRGPKLPYTPGGDAGGVVEAVGPGVTAVKAGDRVYVGGTVTGAYAELAVCE
                     QAQVHPLAANASYAQGAAMNVPYATAYHALFNRARGVAGETVLVHGASGGVGIGAVQL
                     ARARGLTVIGTAGTEKGRRMVLEQGAHHALDHTTAGYLEECVRLTGGRGPDIIMEMLA
                     NVNLQKDLGAIATRGRIVVIGNRGMVEINARLAMNKDADIMGMALNHATPAQHTGIHA
                     ALVEGLRNGSLRPVIAQELPLAQAARSHEAVMEAGHHGKVVLIP"
     gene            complement(1992..2240)
                     /locus_tag="VNU03_17285"
     CDS             complement(1992..2240)
                     /locus_tag="VNU03_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76279.1"
                     /translation="MNLFRSEDHARRWSQFTPRAEEGFITLTDLAGFFGTESRRHMLD
                     PDYLSVWYPRRAVERRAYLESIGKTSPFWMGTPDPPTS"
     gene            complement(2247..2714)
                     /gene="merB"
                     /locus_tag="VNU03_17290"
     CDS             complement(2247..2714)
                     /gene="merB"
                     /locus_tag="VNU03_17290"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HWP76280.1"
                     /translation="MADAKLMDRAFQRIMRSLVETGRAPHYAELGRALGLPAEEGRAL
                     LRDVMAAYPIGWLHPETDYIASFPPLNNLPTQYRITVGGEQRWFAQCGFEATSATWLF
                     PGQTVRVDAACLDCGDPVTVEMRDGRITWADPPDLVGHLAFGFGASRGRSYYL"
     gene            complement(2739..3137)
                     /locus_tag="VNU03_17295"
     CDS             complement(2739..3137)
                     /locus_tag="VNU03_17295"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP76281.1"
                     /translation="MAAYEVITGGSAWPSRYTFSPAVRAGNLLFISGTTAGDEHGQLV
                     GPGDIVAQTRYIFEKFGRLLRAAGGGFEHIVETTDYVTTTENYARTAGVRREFFGERF
                     PTATGVIVAGLLREGALIEISAVAVLPDPS"
     gene            complement(3167..>4784)
                     /locus_tag="VNU03_17300"
     CDS             complement(3167..>4784)
                     /locus_tag="VNU03_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP76282.1"
                     /translation="SRPTYRDACQAFRWEESARALDWAPGEPVNLGHTIVDRHAGGGR
                     PALRWLGRRGEDRTYSFDELGELTARFAAVLRTQGVKPGDRVAGFLPRLPEMLIAMIG
                     TWKAGAVYVPIFTGFGPDAVEYRVRHSGARVLVTHREHRSRLPEPVPPSVTVVTVAGG
                     DAAAPGDTDFWHAMDRHREGAESVVCRRDEPTVLLYTSGSTGPPKGVKIAANFLMAIH
                     PHLVHGADLQPDDVFWPTGDPGWGYGLVCYMGALALGVPVLSFEAAPTPELCVSLLRD
                     RRVSNLATTPTLLRGVMALGPDAVAGVRLRAASSCGEPLNAEVVRFFREHWGVTVMDQ
                     YGSSEFGLPVGNFGTVDMVVKPGSMGLPLPGSTVAVVDDDGHEVGPDVVGHVGMTPHP
                     EGYYSLGYWNHAERDREAYRGRWLTCGDLARRDDDGYFWFEGRADDVIKSAGYRIGPF
                     EVESAILKHEAVAEAAVVGKPDPLRGHIVKAYVVLKPGRHAKTGLADEIAEVVKRHVG
                     RHQYPREVEFLDRLPKTETGKIQRFLLRGR"
BASE COUNT          671 a         1748 c         1682 g          683 t
ORIGIN      
        1 gagccggtct caacagcagc ctcgatcccg aggtggtgtg cgcgcagttc ggctggtggc
       61 aggcgtgccc gccgctcggc ctgcccggct acgacgccga cggccccgga agcgccaact
      121 acaatgccct catcgatccg gaggccaccg acccgatcag cgggacggtg gcgctccgct
      181 cgtacctctg cgaggtacgc cgaggagacc cgtgagcatc gaccaagcga tcgacgcgtt
      241 ctggcaatgc gcccggcagc ggaccgcccc cggtcccgag tggatgaagc gcctgagccg
      301 cgaggaggcg taccgcgtgc agctgggcgt cctcgcccgc tacgtcgccg cgggcgagcg
      361 ccaggcgggc tggaaggtgg gcctcacctc gaaggcgatg caggcccagc agggcgtgca
      421 cgagccggtg ctgggcttcc tcctgcagag cggcgagcag ccgagcggca cgcgcttccg
      481 ctacgcggac ctgatccgcc ccggcttcga gaacgagctc tgcctcacgg tcggcaccac
      541 cctgcgcggc cccggggtga ccaccgagcg ggccgccgca gccctcgccg cgatggcacc
      601 cgccttcgag atcgtcgagc ggcgcggcga cttcgcggcc gatctcaacg tgagcctcgc
      661 cgacaacgcc cagcagcgcg cctacgtcac cggcccgccg gtcccggtgc cgcccggctt
      721 cgacctcgcg acggtggtgg tggagctctc cgtgaacggc gcggcggccg agcgcgcgct
      781 cggcagcgag gtgctcggca cgcccgcagc ctcgatcgcc tggctcgcca acaagctggc
      841 cgagctcggc ctggccctcg aggccggcat gaaggtcatg tcgggctcgt tcacccgcca
      901 gtacctcctc aatgcgggcg accgcatcga agcctcattc accccggtcg gccaggtccg
      961 cgccgagttc atctagcctc gaggcggctc aagggatgag gaccaccttg ccgtggtggc
     1021 ccgcctccat caccgcctcg tggctccggg ccgcctgggc gagcgggagc tcctgcgcga
     1081 tgaccgggcg cagcgagccg ttgcgcaggc cctccaccag cgcggcgtgg atgccggtgt
     1141 gctgggcggg cgtggcgtgg ttgagcgcca tgcccatgat gtcggcatcc ttgttcatgg
     1201 ccaggcgcgc gttgatctcg accatgccgc ggttgccgat caccacgatg cggccacggg
     1261 tggcgatggc gccgaggtcc ttctggaggt tcacgttggc gagcatctcc atgatgatgt
     1321 cggggccgcg gccgccggtg aggcgcacgc actcctcgag gtagcccgcc gtggtgtggt
     1381 cgagcgcgtg gtgcgcgccc tgctccagga ccatccgccg gcccttctct gtcccggcgg
     1441 tgccgatcac ggtgagcccg cgcgcccgcg cgagctgcac cgccccgatg cccaccccgc
     1501 cgctcgcccc gtgcaccagc accgtctccc ccgccacgcc acgggcgcgg ttgaagagcg
     1561 cgtgataggc ggtcgcgtac gggacattca tggccgcccc ctgcgcgtag ctcgcgttgg
     1621 cggccagcgg gtgcacctgc gcctgctcgc acaccgccag ctccgcgtac gctccggtca
     1681 cggtgccacc cacgtagacg cggtcgcccg ccttcaccgc ggtgacgccc ggccccaccg
     1741 cctcgaccac gccgcctgcg tcgccgcccg gggtgtaggg aagcttcggc ccccggttgt
     1801 cggtgttcga gcgcaggtag gtgtccaccg ggttgatgcc gacggcgtgg tggcgcacga
     1861 ggacctgccc cggccccggc tggggcgtgg ggatctcctc gagcttcagg accgacgggc
     1921 caccgtattc cgagacgcgg atcgctttca tgcgggtgtc ctccttaagg atgtgggctc
     1981 aggacgtggg ctcaggacgt ggggggatcg ggagtgccca tccagaacgg cgaggtcttg
     2041 ccgatcgact ccagataggc gcggcgctcg accgcgcggc gcgggtacca caccgacagg
     2101 tagtccgggt ccagcatgtg ccggcggctc tcggtgccga agaagccggc caggtcggtg
     2161 agggtgatga agccctcctc ggctctcggc gtgaactggc tccagcggcg cgcgtggtct
     2221 tccgaccgga agaggttcat cccggttcag aggtagtacg accggccgcg cgaggcgccg
     2281 aagccgaagg cgaggtggcc aaccaggtcg ggcgggtcgg cccaggtgat gcggccgtcg
     2341 cgcatctcga ccgtcaccgg atcgccgcag tcgaggcagg ccgcgtcgac ccgcacggtc
     2401 tggcccggga agagccacgt cgcggaggtc gcctcgaagc cgcactgcgc gaaccaccgc
     2461 tgctcgccgc cgaccgtgat ccggtactgg gtgggcaggt tgttgagcgg cgggaacgac
     2521 gcgatgtagt cggtctcggg atggagccag ccgatcgggt aggcggccat gacgtcgcgc
     2581 aggagcgcgc gcccctcctc ggcgggcagg cccagggccc gcccgagctc ggcgtagtgc
     2641 ggcgcgcggc ccgtctcgac gaggctccgc atgatgcgct ggaacgctcg atccatcagc
     2701 ttcgcgtcgg ccatggtgtc ctcctccggt agaaccggtc aggatgggtc gggcagcacc
     2761 gcgaccgccg agatctcgat cagggcgccc tcgcgcagga gcccggccac gatcacgccg
     2821 gtggcggtcg ggaaacgctc cccgaagaac tcccggcgga cgccggcggt gcgggcgtag
     2881 ttctcggtgg tcgtgacgta gtcggtggtc tccacgatgt gctcgaagcc gccgcccgcg
     2941 gcccggagca gccggccgaa cttctcgaag atgtagcggg tctgggccac gatgtcaccc
     3001 ggcccgacca gctgcccgtg ctcgtctccc gcggtcgtgc ccgagatgaa gaggaggttg
     3061 cccgcgcgca ccgccggcga gaacgtgtag cgggacggcc aggcggaccc gccggtgatc
     3121 acctcgtacg cggccatcgt atcgtcctcc tcgcgtgtgg cggagcctac cgcccccgca
     3181 gcaggaaccg ctggatcttg ccggtctcgg tcttgggcag ccggtcgagg aactcgactt
     3241 cacgagggta ctggtgacgg ccgacgtgcc gcttcacgac ctcggcgatc tcgtccgcga
     3301 gcccggtctt ggcgtggcgg cccggcttca gcaccacgta cgccttcacg atgtgccccc
     3361 gcaacggatc cggcttgccc accaccgcgg cctcggccac cgcctcgtgc ttcaggatcg
     3421 cgctctcgac ctcgaagggg ccgatccggt agcccgcgct cttgatcacg tcgtcggcgc
     3481 ggccctcgaa ccagaagtag ccgtcgtcgt cgcgccgcgc cagatctccg caggtcagcc
     3541 accggccccg gtacgcctcg cgatcccgct ccgcatggtt ccagtagccg agcgagtagt
     3601 agccctccgg gtgcggcgtc atcccgacgt ggccgaccac gtccgggccc acctcgtgtc
     3661 cgtcgtcgtc caccaccgcc accgtggagc ccggcagcgg cagccccatc gagccgggct
     3721 tcaccaccat gtccacggtg ccgaagttgc cgaccggcag gccgaactcg ctcgagccgt
     3781 actggtccat cacggtcact ccccagtgct cgcggaagaa gcggacgacc tcggcgttca
     3841 ggggctcccc gcagctgctg gccgcgcgca ggcggacgcc cgccaccgcg tcgggcccga
     3901 gcgccatcac gccgcgcagc agcgtcgggg tcgtcgcgag attgctgacg cgccggtccc
     3961 ggagcagact cacgcagagc tccggcgtcg gagcagcctc gaacgagagc acgggaaccc
     4021 cgagggcgag cgcgcccatg tagcacacga ggccgtagcc ccagcccgga tcgccggtcg
     4081 gccagaacac gtcgtccggc tgcagatcgg caccgtggac gaggtgcgga tggatcgcca
     4141 tcaggaagtt ggccgcgatc ttgacgccct tcggcggccc ggtcgagccc gaggtgtaga
     4201 gcagcaccgt cggctcgtcg cgccggcaga cgactgattc agccccttcc cggtgacgat
     4261 ccatcgcgtg ccagaagtcc gtgtcaccgg gcgccgcggc gtcgccgccg gcgaccgtga
     4321 cgacggtcac gctgggcggc accggctcgg gcaggcgcga gcggtgctcg cgatgcgtga
     4381 ccaggacgcg cgccccgctg tggcgtaccc ggtactccac cgcgtccggg ccgaatccgg
     4441 tgaagatcgg cacgtacacc gcccccgcct tccacgtgcc gatcatcgcg atcagcatct
     4501 cgggcaggcg gggcaggaag cctgcaaccc ggtcgcccgg ctttacccct tgagtgcgga
     4561 gcacggccgc aaagcgagcg gtcagctcgc cgagctcgtc gaacgaatag gtccggtcct
     4621 cgccgcgcct gccgagccat cgaagcgcgg ggcgcccgcc gcccgcgtga cggtcgacga
     4681 tggtgtggcc gagattcacc ggctcgccgg gggcccagtc gagggcgcga gcactttcct
     4741 cccagcggaa ggcctggcag gcatcgcgat aggtcggccg gctc
//
LOCUS       DATMCV010000754         3235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_443436, whole genome shotgun sequence.
ACCESSION   DATMCV010000754 DATMCV010000000
VERSION     DATMCV010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443436"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1023
                     /locus_tag="VNU03_17305"
     CDS             115..1023
                     /locus_tag="VNU03_17305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76283.1"
                     /translation="MAIGTLAPPMGAAAGSAHAAERSRVWRKFARNPAAIGGALILLA
                     VVGAAIFAPYVAPHEPTRQSLIRRFTPPVWTAGGKASYVLGTDQVGRDILSRMIHGAR
                     VSLMVGVAAVLVSLTVGVTLGLMSGFLRGRVDTVIMTVVDVTLSFPQLLLALAFVAAL
                     GPSLLTIIVVLGLTGWERYTRVVRAEVLALREKDFVEAARAIGASSTRTLLRHLLPNT
                     FSSIIVMSTLQVAQAILQEAALSFLGVGTGSAYPTWGQMIALGRDFVTVAWWLPTFPG
                     LAILLTVLAINLVGDRLRDALDPRVG"
     gene            complement(1020..2126)
                     /locus_tag="VNU03_17310"
     CDS             complement(1020..2126)
                     /locus_tag="VNU03_17310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP76284.1"
                     /translation="MPGAAEIVVVGAGCVGSSVAYRLAQRGARVTLLDAGAPGAGTSG
                     TSFAWTNSFGKTPRDYHDLNVAGMEEHAALVKELKELPGGGWLHQDGALAWEDSPVGC
                     ARLDQAVERLAGWGYAIERITPAQAREIEPDLHIASDVARVIWTPGEGYLDAVPYVAA
                     LLAAARRHGARVRPGRRVAEVLRVADRVVGVRTEDGERFEARVVVDCAGVAAGEVARL
                     AGLEVPLDRVPGRLIYTSPVATTLRRPIHAPGVHFRPDGGGRVVLAEGTHDQVWRETV
                     EAWPPERSLAAVAVHLPALAAARVEATRVGLRPMPRDERPMVGGIPGLDGFYVVVSHS
                     GVTLGPLWGRIAAAELLDGVPDPRLAPYRPARFL"
     gene            complement(2170..2805)
                     /locus_tag="VNU03_17315"
     CDS             complement(2170..2805)
                     /locus_tag="VNU03_17315"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWP76285.1"
                     /translation="MSLRLGDTAPDFTAETTQGPVKLHDWIGDSWAILFSHPRDYTPV
                     CTTELGYVARLKGEFDKRQVKCIGLSIDPVDSHKGWMKDIKETQGHEPTFPIVADPER
                     KVANLYGMMHPAHDEVYTVRTVFVIDPKKKIRLMITYPQTTGRNFDEILRVIDSMQLT
                     DGYRVATPVNWKQGDDVIIVPAMTDDEAKTKFPKGWKALKPYLRLTPQPNK"
     gene            complement(2822..>3235)
                     /locus_tag="VNU03_17320"
     CDS             complement(2822..>3235)
                     /locus_tag="VNU03_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HWP76286.1"
                     /translation="RGRGPDGGRGRFMLHRAPRFNVTAVTWRPGDRAAAHNHDTWGVI
                     GVVDNEIEETRYRVAEGPGGGAALTVWSVTRHGAGAISLLVPPDDEVHAMYNPTGRDT
                     VEIHVYGRDLYGLPRKTWAADGRVSPLVSPKYLNC"
BASE COUNT          410 a         1148 c         1177 g          500 t
ORIGIN      
        1 cccgatcgtg caggccggcg tcttcgtcat tgccctgatc gtggtgctgg tgaacttcgc
       61 agtggacatg ctgtacatct acctgaaccc gcagatccgg gtgcgctgaa ggccatggcc
      121 atcggcaccc tcgcgccgcc gatgggagcc gcggccggct ccgcgcatgc cgcggagcgc
      181 tcccgagtct ggcgcaagtt cgcacgcaat cccgccgcga tcgggggcgc cctcatcctc
      241 ctcgccgtag tcggcgccgc catcttcgcg ccctacgtcg cgccgcacga gcccacccgc
      301 cagagcctga tccgtcgctt caccccgccg gtctggaccg cgggaggcaa ggcgagctac
      361 gtgctcggta cggaccaggt tggccgcgac atcctgagcc gcatgatcca cggcgcgcgc
      421 gtctcgctca tggtcggggt cgccgcggtc ctggtcagct tgacggtggg ggtcaccctg
      481 gggctgatga gcggcttcct gcgggggcgg gtggacaccg tgatcatgac ggtggtggac
      541 gtgaccctgt cgtttccgca gctcctgctc gcgctcgcct tcgtggccgc gctcggcccg
      601 agcctgctca ccatcatcgt ggtgctcggc ctcaccgggt gggagcgcta cacgcgggtg
      661 gtgcgcgccg aggtgctcgc gctccgcgag aaggacttcg tggaggccgc gcgggccatc
      721 ggggccagct ccacgcgcac gctgctgcgg catctgctgc cgaatacctt ctcgtccatc
      781 atcgtgatgt ccacgctgca ggtcgcgcag gcgatcctgc aggaggccgc gctctccttc
      841 ctcggggtgg ggacggggag cgcctacccg acctggggcc agatgatcgc gctggggcgc
      901 gacttcgtga cggtggcctg gtggctcccc accttcccgg gcctggccat cctgctcacc
      961 gtgctcgcca tcaacctggt cggcgatcgg ctgcgcgacg cgctggatcc gcgggtgggc
     1021 tagaggaagc gggcggggcg gtagggagcg aggcgcgggt cgggcacgcc gtcgagcagc
     1081 tcggccgccg cgatgcggcc ccacagcggc cccagcgtga ccccgctgtg cgagaccacc
     1141 acgtagaagc cgtcgagccc cgggatccca ccgaccatcg ggcgctcgtc gcgcggcatg
     1201 gggcgcagcc cgacgcgggt ggcctcgacc cgcgcggcgg ccagcgcggg caggtggacg
     1261 gccaccgcgg ccagcgagcg ctccggcggc caggcctcga ccgtctcccg ccacacctgg
     1321 tcgtgcgtgc cctccgccag cacgacgcgc ccgcccccgt ccggccggaa gtgcacgccg
     1381 ggcgcgtgga tgggccgccg cagggtggtg gcgaccgggg aggtgtagat gagccgcccc
     1441 gggacgcggt cgagcggaac ctcgaggccg gcgagacggg cgacctcacc ggccgcgacg
     1501 cccgcgcagt ccaccaccac ccgggcctcg aagcgctcac cgtcctcggt gcgcacgccc
     1561 accacgcggt cggcgacccg cagcacctcc gcgacgcggc ggccgggccg cacccgcgcc
     1621 ccgtggcgtc gggcggcggc cagtagcgcg gcgacgtagg gcaccgcgtc caggtacccc
     1681 tcgcccggcg tccagatcac ccgggcgacg tcggaggcga tgtggagatc gggctcgatc
     1741 tcgcgggcct gcgcgggcgt gatgcgctcg atcgcgtagc cccacccggc gagccgctcg
     1801 accgcctgat cgagccgggc gcatccgacg ggggagtcct cccacgcgag cgcgccgtcc
     1861 tggtggagcc accctccgcc gggcagctcc ttcagttcct tcacgagggc ggcgtgctct
     1921 tccatgcccg cgacgttgag atcgtgatag tcgcgcgggg tcttgccgaa ggagttggtc
     1981 caggcgaacg aggtgccgga ggtgcccgcg cccggcgcgc ccgcgtccag cagggtgacg
     2041 cgggcgccgc gctgggccag gcggtaggcg acactggagc cgacgcaccc ggccccgacc
     2101 acgacgatct cggcggctcc tggcatgagc gggcgagcta ggccgggtcg gggcgcggag
     2161 cgcgccgcgc tacttgttgg gctgcggggt gaggcgcagg tagggcttga gcgccttcca
     2221 gcccttgggg aacttggtct tggcctcgtc gtcggtcatg gcgggcacga tgatgacgtc
     2281 gtcgccctgc ttccagttga ccggggtggc cacgcggtag ccgtcggtga gctgcatcga
     2341 atcgatcacc cgcaggatct cgtcgaagtt ccgcccggtg gtctggggat aggtgatcat
     2401 cagccggatc ttcttcttgg ggtcgatcac gaacacggtg cgcaccgtgt agacctcgtc
     2461 gtgggccgga tgcatcatgc cgtagaggtt tgcgaccttg cgctccgggt cggccacgat
     2521 cgggaaggtg ggctcgtggc cctgcgtctc cttgatgtcc ttcatccagc ccttgtgcga
     2581 gtccaccggg tcgatcgaga gcccgatgca cttgacctga cgcttgtcga actcgccctt
     2641 gagccgcgcc acgtacccga gctcggtggt gcagaccggc gtgtagtcgc gcgggtggga
     2701 gaacaggatg gcccaggaat cgccgatcca gtcgtgaagc ttgaccgggc cctgggtggt
     2761 ctcggcggtg aagtcgggtg cggtgtctcc gagtctcagc gacattgctt ctcctccatc
     2821 gtcagcagtt gaggtatttc gggctcacca gcgggctcac gcgcccgtcg gcggcccagg
     2881 tcttgcgcgg caggccgtag aggtcgcgcc cgtagacgtg gatctcgacg gtgtcgcgcc
     2941 cggtgggatt gtacatcgcg tggacctcgt cgtccggagg aacgaggagc gagatcgccc
     3001 cggccccgtg gcgggtcacg ctccaaaccg tcagcgcggc gcccccgccc ggaccctcgg
     3061 ccacccggta acgggtctcc tcgatctcgt tgtccaccac gccgatgacg ccccacgtgt
     3121 cgtggttgtg ggccgccgcg cggtcgccgg gccgccaggt caccgcggtg acgttgaagc
     3181 gcggcgcccg gtggagcatg aagcggccgc ggccgccgtc ggggccgcgc ccgcg
//
LOCUS       DATMCV010000755         5537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_307082, whole genome shotgun sequence.
ACCESSION   DATMCV010000755 DATMCV010000000
VERSION     DATMCV010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307082"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VNU03_17325"
     CDS             complement(<1..400)
                     /locus_tag="VNU03_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76287.1"
                     /translation="MSLSPAAVLGAPAVPATRRYKAARQLARRPAAMAALAVVLGFIV
                     VAVAAPHLAPYDPNAIDYRALRKAPSAAHLLGTDEVGRDVLSRLLWGARASLLAGVVP
                     VTIAVALSVPLGLLSGSVGGWVDGLIMRIND"
     gene            complement(397..1350)
                     /locus_tag="VNU03_17330"
     CDS             complement(397..1350)
                     /locus_tag="VNU03_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76288.1"
                     /translation="MALWLYVVRRLAVLVPTLFFVSVLIFSLQQLLPGDAAVALAGEE
                     HDPTVVAAIRARYHLDRPLPVQYGLWLGRVLVGDFGESLRNRIPVRELLASKLPVTVE
                     LALCSMLIALVIGIPTGILSATRKGTAVDVVANFFALSGLSVPHFWLGIMLILLFAVR
                     LGWLPASGYVPPWEDLRQNLTTILLPSFVLGTGVAGVIMRHTRSAMLQTLGADYVRTA
                     RAKSVPERLVVLKHALRNALIPIITLGAIEFGRLLSGAVLTEQVFAIPGFGKLLVDGV
                     FNRDYAVVQAVVIVSAGLYVLLNLGADVLYFLANPRLRGST"
     gene            complement(1363..2862)
                     /locus_tag="VNU03_17335"
     CDS             complement(1363..2862)
                     /locus_tag="VNU03_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76289.1"
                     /translation="MKTILTALLAILLLVPARASAQSVLRIGLNDDPDALDPTISRAY
                     TGRLVFAALCDKLFDVTPDLKIVPQLATGFEWGADRKSIVIKLRPGVRFQDGEPFNAE
                     AVKFNIERHQNTPGSFRKPEIGEIQSVKVVNDLTVRLHLSQPLVPLLAALTDRSGMMV
                     SPKAAKALGDKLGTQPVCAGPYKFVQRVAQGKIVMEKVADYWDKNAFSVDRIEFVPIT
                     DSSSRLASLRSGDLQMIERVSPTDLAEIRGDSKLKVTGVPELGYQMIPLNVANGPKSK
                     ALADVRLRQALDLAIDRETLVKTVFGGEYIAGNQFISPESPYYDKKVPVAKRDVAKAK
                     QLLKDAGQPNLTFTLVVPPERDRQEAALILQAMWAEAGITANLQTQENVTMLQSGRKG
                     DFEAYFTFWSGRPDPDGNVYTFSTCKGAQNDMHYCNADVDALLVKARQVADPAERKKL
                     YDQATEIMAKEVPRLILWHRRVFTGLSTRVQGFTPYPDGIIRFRGLKLN"
     gene            3023..3910
                     /locus_tag="VNU03_17340"
     CDS             3023..3910
                     /locus_tag="VNU03_17340"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76290.1"
                     /translation="MKAGLKIIDMECSVPYGAEHEKTAAAAAPPAAGQPGYGMANYGR
                     IFRSRSEGNDPRPKTDLDAYVKHLAGLGIVRSVPFGVSNEEATALLRRYPGRFLGLAR
                     ISVNTHRGMAGVRELERLVRDERFSALGISALVDCLPASDRRYYPLYTKAAELGIPVR
                     IYSSMNYANDRPYDLGHPRHLDQVAMDFPELTIIGGLGGWPWVNEMVALVRRHPKLHM
                     DTSAHRGRYLGQPGSGWEMLMQFGNTLIQDKVLVGLSAGLVGQPYETLIDEYLGLPLK
                     DSVKEKWLYGNAARVFGIA"
     gene            3921..>5537
                     /locus_tag="VNU03_17345"
     CDS             3921..>5537
                     /locus_tag="VNU03_17345"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP76291.1"
                     /translation="MTRLAGLPGGDRGGALADVLVVELTHTLAGEMAGGLLADLGATV
                     VKVEPAAGSPMRKRGPALPGEDSLYFQSENRGKLSVQAESADLAREPWLATLLASADA
                     LIEDLGPGRLEAAGLAPAELERRNPRLALLRISPFGQTGPLAAERGDDRIAQAFSGVQ
                     FTTGFPDRAPMPVTVPLAEAWTAIQGASGLLMAVFHARRRGHGQVVDIGLYQMGLRLQ
                     EEVVVRHARSGEVATRLGTESPVVVPANVYRTLDGGWVAVSGAGDQPFARLCEAIEAP
                     EAPRDPRFATSAARLQNRAAADALVGDWIARHDQLEVEARFAAVGVAGAAVRSADDIL
                     ADEHVKARRALLQLASTGGQLFVAPGAVPKLTRTPAADPSPAPRLGEHTAAVRGAMER
                     APAPPTRPPAPVESVEPVAAGALAGLRVLDLSQWLAGPAAAAILGDFGADVIMIELPP
                     AGGTSDTWARTSPGTVVTNRNKRSLALDVRSPRGRELFLELVRVSDVVVENFRPGTLE
                     RWKLGPEVLLETNPKLVLLRSSGFGQTGPYT"
BASE COUNT          796 a         1953 c         2025 g          763 t
ORIGIN      
        1 cgtcgttgat gcgcattata agcccgtcga cccagccccc gacagagccg gagagcagcc
       61 cgagcggcac gctgagcgcg accgcgatgg tgaccgggac gacgccggcc agcagcgacg
      121 cccgcgcgcc ccagagcagg cgcgacagca cgtcgcgccc cacctcgtcg gtgccgagga
      181 ggtgcgcggc ggacggcgcc ttgcggagcg cccggtagtc gatggcgttc ggatcgtacg
      241 gggcgaggtg cggcgcggcc accgccacca cgatgaagcc gagcaccacc gccagcgcgg
      301 ccatcgccgc gggccggcgg gccagctgcc gcgccgcctt gtagcgtcgc gtcgcgggca
      361 ccgcgggcgc cccgaggacc gccgccgggc tcaggctcac gtgctccccc gcaggcgcgg
      421 gttggccagg aagtagagca cgtccgcgcc caggttcagg agcacgtaga ggcccgccga
      481 gactatgacg acggcctgca cgacggcgta gtcgcgattg aacacgccgt ccaccagcag
      541 cttgccgaag ccggggatgg cgaagacctg ctcggtgagc accgcgcccg agagcagccg
      601 cccgaactcg atcgcgccca gggtgatgat cgggatcagc gcgttgcgga gcgcgtgctt
      661 cagcaccacc aggcgctcgg gcacactctt ggcccgtgcg gtgcgcacgt agtcggcgcc
      721 cagggtctgg agcatggcgc tgcgggtgtg gcgcatgatg accccggcga ccccggtgcc
      781 gaggacgaac gagggcagca ggatcgtggt caggttctgg cgcaggtcct cccacggcgg
      841 cacgtagccg gaggcgggaa gccagccgag gcgcaccgcg aacagcaaga tgagcatgat
      901 gcccagccag aagtgcggca ccgacagccc ggagagcgcg aagaagttgg ccaccacgtc
      961 caccgcggta cccttgcggg tcgcggacag gatgccggtc gggatgccga tgacgagcgc
     1021 gatcagcatc gagcagagcg cgagctcgac ggtgaccggc agcttggagg ccagcagctc
     1081 gcgcaccgga atgcggttgc gcagcgactc gccgaagtct ccgaccagca cgcggccgag
     1141 ccagagcccg tactgcaccg gcagcggccg gtccaggtgg taccgcgccc ggatggccgc
     1201 gaccaccgtc gggtcgtgct cctcgccggc cagcgccacc gccgcgtcgc cgggcagcag
     1261 ctgctgcagc gagaagatca gcaccgacac gaagaacagt gtggggacga gcaccgccag
     1321 ccggcgcacc acgtagagcc agagcgccat gccgccggcg cctcagttca gcttgaggcc
     1381 ccggaagcgg atgatcccgt cggggtaggg cgtgaagccc tggacccggg tgctgaggcc
     1441 ggtgaagacg cgccggtgcc acaggatcag ccgcggaacc tccttggcca tgatctcggt
     1501 ggcctggtcg tagagcttct tgcgctcggc cggatccgcg acctggcggg ccttgacgag
     1561 cagcgcgtcc acgtcggcgt tgcagtagtg catgtcgttc tgcgcgccct tgcaggtcga
     1621 gaaggtgtag acgttgccgt cggggtcggg gcggccgctc cagaacgtga agtaggcctc
     1681 gaagtcgccc ttgcgcccgg actgcagcat cgtcacgttc tcctgggtct ggaggttcgc
     1741 ggtgatgccg gcctccgccc acatcgcttg taagatcagc gccgcctcct ggcggtcgcg
     1801 ctccgggggc accaccagcg tgaacgtgag attgggctgg cccgcgtcct tcaggagctg
     1861 cttcgccttg gccacgtcgc gcttggccac cgggaccttc ttgtcgtagt agggactctc
     1921 ggggctgatg aactggttgc ccgcgatgta ctcgccaccg aacacggtct tgaccagggt
     1981 ttcccgatcg atcgcgaggt cgagggcctg acgcagccgg acgtccgcga gcgccttgct
     2041 cttcgggccg ttggcgacgt tcagcgggat catctggtag cccagctcgg gcacgccggt
     2101 gactttgagc ttggaatccc cacggatttc cgcgaggtcg gtcggcgaca cgcgctcgat
     2161 catctggagg tcgccggagc gcaggctggc caggcgcgag ctcgagtcgg tgatgggcac
     2221 gaactcgatg cgatcgaccg agaaggcgtt cttgtcccag tagtcggcca ccttctccat
     2281 cacgatcttg ccctgggcca cccgctgcac gaacttgtac ggcccggcgc agaccggctg
     2341 ggtgcccagc ttgtcgccga gcgccttcgc cgccttgggc gagaccatca tgccggagcg
     2401 gtcggtcagg gcggccagga ggggcaccag cggctgcgag agatggagcc gcaccgtgag
     2461 gtcgttcacc accttgacgg actggatctc gccgatctcc ggcttgcgga acgagccggg
     2521 cgtgttctgg tgccgctcga tgttgaactt gaccgcctcc gcgttgaacg gctcgccgtc
     2581 ctggaagcgg accccgggcc gcagcttgat cacgatcgac ttgcggtccg ctccccactc
     2641 gaagccggtg gcgagctggg gcacgatctt gagatccggg gtgacgtcga agagcttgtc
     2701 gcaaagggcg gcgaagacca gccggccggt gtaggcgcgg ctgatggtgg ggtcgagggc
     2761 gtcggggtcg tcgttgagcc cgatgcggag cacgctctgc gcgctcgccc gggcgggcac
     2821 cagcagcagg atcgcgagca gcgcggtcag gatcgtcttc acggggtctc cttggtccga
     2881 tcggggatgc cgggcatggc cgagctccgg gtgatgccgg gatactgcgg cgcgggggcg
     2941 gtactgtcaa ggcggccgtg atcgcggtac tgtatgcggc gcgcccgcga agcaggcact
     3001 cggccggcgc gggaggcacc acatgaaggc agggctcaag atcatcgaca tggagtgcag
     3061 cgtcccctac ggggcggagc acgagaagac cgccgccgcg gccgccccgc ccgccgccgg
     3121 ccagcccggc tacggcatgg ccaactacgg ccgcatcttc cgctcgcgca gcgagggcaa
     3181 cgacccgcgc ccgaagaccg acctggacgc ctacgtgaag cacctcgcgg ggctcggcat
     3241 cgtccgctcg gtgcccttcg gcgtctcgaa cgaggaggcc accgcgctgc tgcgccgcta
     3301 tcccggccgc ttcctcggcc tggcccggat cagcgtcaac acgcaccggg gcatggccgg
     3361 cgtccgcgag ctggagcgcc tcgtgcgcga cgagcgcttc agcgccctcg gcatctcggc
     3421 cctggtcgac tgcctgcccg cgagcgatcg ccgctactac ccgctctaca ccaaggccgc
     3481 cgagctcggc atcccggtgc gcatctactc gtcgatgaac tacgccaacg accgcccgta
     3541 cgacctgggc cacccgcgcc acctcgacca ggtggcgatg gatttcccgg agctcaccat
     3601 catcggcggc ctcggcggct ggccgtgggt gaacgagatg gtggcgctgg tgcgacggca
     3661 tccgaagctg cacatggaca cctcggccca ccgcgggcgc tatctcggcc agccgggctc
     3721 gggctgggag atgctgatgc agttcggcaa cacgctgatc caggacaagg tgctggtcgg
     3781 cctctcggcc gggctcgtgg ggcagccgta cgagacgctg atcgacgagt acctcggcct
     3841 gccgctcaag gactcggtga aggagaaatg gctctacggc aacgcggcgc gcgtcttcgg
     3901 gatcgcgtga ggcgcccgcg gtgacgcggc tcgcggggtt gccgggcggc gatcgcggcg
     3961 gcgcgctggc cgacgtgctg gtggtcgagc tgacgcacac gctcgccggt gagatggcgg
     4021 gcggcctgct cgccgatctg ggcgccaccg tcgtcaaggt cgagcccgcc gccggctcgc
     4081 cgatgcggaa gcgcggcccc gcgctgcccg gcgaggactc gctctatttc cagtccgaga
     4141 accgcggcaa gctctcggtc caggccgagt cggccgacct cgcgcgcgag ccgtggctcg
     4201 cgacgctgct cgcctccgcg gacgcgctga tcgaggacct ggggccggga cgcctcgagg
     4261 cggccggcct cgcgccggcc gagctcgagc ggcgcaaccc gcgcctcgcc ctcctgcgca
     4321 tctcgccctt cggccagacc gggccgctcg cggccgagcg cggcgacgac cgcatcgccc
     4381 aggcgttctc gggcgtgcag ttcaccaccg gcttcccgga ccgcgcgccg atgccggtga
     4441 cggtgccgct ggccgaggcc tggaccgcga tccagggcgc gagcgggctc ctgatggcgg
     4501 tcttccacgc gcggcggcgc gggcacgggc aggtggtcga catcggcctc taccagatgg
     4561 gcctccggct gcaggaagaa gtcgtggtcc gccacgcgcg gtccggtgag gtggcgacgc
     4621 gcctcggcac cgagtcgccg gtggtggtgc cggccaacgt ctatcgcacg ctcgacggcg
     4681 gctgggtcgc ggtctcgggc gccggcgatc agcccttcgc gcgcctctgc gaggccatcg
     4741 aggcgccgga ggcgccgagg gacccgcgct tcgccaccag cgccgcgcgt ctgcagaacc
     4801 gcgcggcggc ggacgcgctg gtcggcgact ggatcgcccg ccacgaccag ctggaggtgg
     4861 aggcgcgctt cgcggcggtc ggcgtcgcgg gcgccgcggt gcggtcggcg gacgacatcc
     4921 tagccgacga gcacgtgaag gcgcgccggg cgctgctcca gctggcgtcg accggagggc
     4981 aactgttcgt ggcgcccggg gcggtgccga agctcacgcg cacgccggcg gcggatccgt
     5041 cgccggcgcc gcgcctcggc gagcacaccg cggcggtgcg cggggcgatg gagcgcgcgc
     5101 cggcgccgcc gacccggccg cccgccccgg tcgaatcagt cgagccggtc gccgccggcg
     5161 ccctcgcggg gctccgcgtc ctcgatctct cccagtggct cgccggcccc gccgccgcgg
     5221 ccatcctcgg cgacttcggc gccgacgtga tcatgatcga gctgccgccg gccggcggga
     5281 cgtcggacac gtgggcgcgc accagcccgg gcaccgtggt caccaaccgc aacaagcgca
     5341 gcctggcgct cgacgtgcgc agcccgcgcg ggcgcgagct cttcctcgag ctggtgcgcg
     5401 tgagcgacgt ggtcgtggag aacttccggc ccggcacgct cgagcgctgg aagctcggcc
     5461 ccgaggtgct gctcgagacc aacccgaagc tggtgctgct gcgctcctcc ggcttcggcc
     5521 agaccgggcc ctacacc
//
LOCUS       DATMCV010000756         5415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_107110, whole genome shotgun sequence.
ACCESSION   DATMCV010000756 DATMCV010000000
VERSION     DATMCV010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107110"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VNU03_17350"
     CDS             <1..507
                     /locus_tag="VNU03_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76292.1"
                     /translation="SGNRMAFATLELVDGSVPLTIFPEPYRTCATALRHKGPVIVKGR
                     ADDSDKGRVVLAEEIKPLEEAVANGNGNGNGNGNGHRGEAEVAHACRIRVSAAAESLD
                     ALFASLKAACREHEGRTPLFVHVLLPEQEVVLRIKELGVEPAPALVAKVEGLLGPGSI
                     LVEYAGRA"
     gene            491..1444
                     /locus_tag="VNU03_17355"
     CDS             491..1444
                     /locus_tag="VNU03_17355"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HWP76293.1"
                     /translation="MPDELEFEKPLLELENRIAELRASEDSLAARDEIAKLEERLARQ
                     QQRVYSGLTAWQRTQIARHPKRPHTLDLVNLLLEDWVELHGDRVFGDDKAIVGGIATF
                     DGEPVVVIGHQKGRDTRENIARNFGMPHPEGYRKALRLMQLAGKFGKPIITLIDTPGA
                     YPGLAAEERGQAEAIARNLREMAGLPTPVICVVTGEGGSGGALALGVGNRVLMLEYSI
                     YSVISPEGCAAILWGEAAKAPEAAELMRVTAPDLLKLGVIDAIVPEPVGGAHRNWDAT
                     AASLREALREQLWQLRSKSEAELIEERQEKFRRIGVFEETV"
     gene            1510..2913
                     /gene="miaB"
                     /locus_tag="VNU03_17360"
     CDS             1510..2913
                     /gene="miaB"
                     /locus_tag="VNU03_17360"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWP76294.1"
                     /translation="MQKNPPLSPRKLHLITYGCQMNEYDSERVAGLLKQERYELTDSA
                     EEADLILLNTCSIREKAEDKVFSQLGALRLLKRRRPDLTIGVMGCMAQLQKGRIQERA
                     PYVDLVFGSPAIARVAELVERAREGRGPILETGESPLVKITARPDGGRRLKAFVTVME
                     GCEKFCTFCVVPTTRGRERSHAPEVIVEEIRGLAAAGCREVTLLGQTVNAYGRDLDPA
                     TDLAALLERVNDVDGIERIRFTTSNPYNLTPRLIRALRDVPKVCEYLHLPLQSGSDRV
                     LERMNRGYSRARYLELIAELQETVPDLALSTDLIVGFPGETEDDFAQTVEMVERVGYD
                     NVFVFRYSRRPGTPAANMADQVPDEVKALRNTRLLEVTGRVAAARSLRLEGRTLPVLV
                     DGVSRKNAGEAAGRTRCNRVVNFDTGGRDLLGRVVEVRIARALPHSLRGELVDASGDP
                     GAMDRLPVGLAEPALPA"
     gene            2927..3460
                     /locus_tag="VNU03_17365"
     CDS             2927..3460
                     /locus_tag="VNU03_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HWP76295.1"
                     /translation="MVLEMKVKGLALDPLSNMPMIILRDEEEKRSLPIWVGLFEANAI
                     ALELEKISTPRPMTHDLIKNILEALDARVQKVVVNDLRDNTFYAVLYLRLGNADITVD
                     SRPSDAIALALRVGAPIFVEEDVVDKARTVEVAKETEIGTGKGDDQAKIKEWLESLKP
                     GDFDRLERDKDPKRPDE"
     gene            3464..4612
                     /locus_tag="VNU03_17370"
     CDS             3464..4612
                     /locus_tag="VNU03_17370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP76296.1"
                     /translation="MRVGLFTNNYLPFRGGVTTAVETLRLGLEGLGHRAWVFAPAAQH
                     PHQDPPFVFRYPSIPAPTYPGFSLPLPVSRRLGRLARSLNLDVVHVHHPFLLGVTGRR
                     LARGQRRPLVFTYHTRYEKYAHYVPLPPRLVRSLAVRLACRFADSTDLVVAPSDHVAD
                     TLRRRGVRAPVAVIPTGVDLSLFSPGSRERARRRLGLPAEGLICLYTGRLDREKSLER
                     VLDAFESVAAALSGATLHLVGKGSHGAALEARAAAGRAGRRVVFHGGLAREALPDYYR
                     AADLFLFASETETQGLVLAEAHACALPAVAVRASGVDEVVTDGETGFLTKGETGEMAD
                     AAIGLLLDVERRVAMGQAARRLAEARFSTGRQIDAMVGHYEALIAGRR"
     gene            4612..4881
                     /locus_tag="VNU03_17375"
     CDS             4612..4881
                     /locus_tag="VNU03_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76297.1"
                     /translation="MPGSTLGRTLRVGLQTDWDPHTAGPGHQGLLAWDQRVRTLLRVL
                     VSYPEVRYILPDRISLEASADPRTLETMARFLERQSWLVRRVAVT"
     gene            4907..>5415
                     /locus_tag="VNU03_17380"
     CDS             4907..>5415
                     /locus_tag="VNU03_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HWP76298.1"
                     /translation="MWNRDVETAPRAALRALQAERLRATVARARARVPFYKDAFAEAG
                     VRDGDVDLERLAELPFTRKAHLRDHYPFGLFAVPREELVRIHASSGTRGKPTVVGYTR
                     GDLELWREVMARALAAAGAEPGQLVQIAYGYGLFTGGLGFHDGAEHMGLTVVPVSSGN
                     TLRQILLLQ"
BASE COUNT          787 a         1935 c         1953 g          740 t
ORIGIN      
        1 agcggcaatc gcatggcctt cgcgacgctc gagctggtcg acggcagcgt gccgctcacg
       61 atcttccccg agccctaccg cacctgcgcg accgcgctgc ggcacaaggg gccggtcatc
      121 gtgaagggcc gggccgacga cagcgacaag ggccgggtgg tgctcgcgga ggaaatcaag
      181 ccgctggagg aagcggtggc aaacggcaac ggaaatggaa acgggaacgg aaacggccac
      241 cgcggcgagg cggaggtcgc gcacgcctgc cgcatccggg tgagcgccgc ggcggagtcg
      301 ctcgacgccc tgttcgcctc gctgaaggcg gcctgccgcg agcacgaggg gcggaccccg
      361 ctcttcgtgc acgtgctgct ccccgagcag gaggtggtgc tgcggatcaa ggagctgggc
      421 gtggagcccg cgcccgccct ggtcgcgaaa gtcgagggcc tcctggggcc gggcagtata
      481 ctcgtcgaat atgccggacg agcttgagtt cgagaagccg ctcctcgagc tcgagaatcg
      541 catcgccgag ctgcgcgcct ccgaggactc gctcgccgcg cgcgacgaga tcgcgaagct
      601 cgaggagcgc ctcgcgcgcc agcagcagcg cgtctactcg ggcctgaccg cctggcagcg
      661 tacccagatc gcccggcacc cgaagcgccc ccacaccctc gacctcgtca acctgctgct
      721 ggaggactgg gtcgagctcc acggcgaccg ggtcttcggc gacgacaagg cgatcgtggg
      781 cggcatcgcc accttcgacg gcgagccggt ggtggtgatc gggcatcaga aggggcggga
      841 cacccgcgag aacatcgccc gcaacttcgg gatgccccat cccgagggct accgcaaggc
      901 gctccgcctg atgcagctgg ccggcaagtt cggcaagccg atcatcacgc tcatcgacac
      961 gccgggggcc tatccgggcc tggccgccga ggagcgcggg caggccgagg ccatcgcgcg
     1021 caacctgcgc gagatggcgg ggctgcccac gccggtgatc tgcgtggtca ccggggaggg
     1081 cggcagcggc ggcgcgctcg cgctcggcgt cggcaaccgg gtgctgatgc tcgagtactc
     1141 gatctactcg gtgatctcgc cggagggctg cgcggccatc ctctggggcg aggccgcgaa
     1201 ggcccccgag gcggccgagc tgatgcgggt gaccgcgccg gacctgctca agctgggggt
     1261 catcgacgcg atcgtgcccg agccggtggg cggcgcccat cgcaactggg acgcgaccgc
     1321 ggccagcctg cgggaggcgc tgcgcgagca gctctggcag ctccggtcca agtcggaggc
     1381 ggagctgatc gaggagcgcc aggagaaatt ccggcggatc ggcgtcttcg aggagaccgt
     1441 ctgagcgttc cgatccgccc ggagtttgac atgcccgggg gcggatgcta ccgtaagtcc
     1501 cgaccaatta tgcagaaaaa tcctccgctg tctccgcgga agctccatct gatcacctac
     1561 ggctgccaga tgaacgagta cgactccgag cgcgtggcgg ggctgctcaa gcaggagcgc
     1621 tacgagctga ccgattccgc ggaagaggcg gacctgatcc tgctcaacac ctgctcgatc
     1681 cgcgagaagg cggaggacaa ggtgttctcc cagctgggcg cgctgcggct cctcaaacgg
     1741 cggcgccccg acctcacgat cggggtcatg ggctgcatgg cccagctcca gaagggccgc
     1801 atccaggagc gcgcgcccta cgtcgacctg gtcttcggct cgcccgcgat cgcccgggtc
     1861 gcggagctgg tcgagcgggc ccgggagggg cggggaccga tcctcgagac cggcgagagc
     1921 ccgctcgtca agatcaccgc gcggccggac ggcggccggc ggctcaaggc gttcgtcacg
     1981 gtcatggagg gctgcgagaa gttctgcacc ttctgcgtcg tgccgaccac ccggggccgg
     2041 gagcgcagcc acgcccccga ggtcatcgtc gaggagatcc ggggtctcgc cgcggcgggc
     2101 tgccgggagg tgaccctgct cggccagacc gtcaacgcct acgggcggga tctcgacccg
     2161 gccaccgacc tggccgccct gctcgagcgg gtgaacgacg tcgacggcat cgagcggatc
     2221 cgcttcacca cctcgaaccc gtacaacctc accccgcgcc tgatccgcgc cctgcgggac
     2281 gtgcccaagg tctgcgagta cctgcacctg ccgctgcagt ccgggtccga tcgcgtgctc
     2341 gagcggatga accgcggcta cagccgcgcc cgctacctcg agctgatcgc ggagctgcag
     2401 gagacggtgc cagacctcgc gctctccacc gacctgatcg tgggcttccc gggcgagacc
     2461 gaggacgact tcgcccaaac cgtggagatg gtggagcggg tgggctacga caacgtgttc
     2521 gtcttccgct attcccggcg gcccggcacg cccgccgcca acatggcgga ccaggtgccg
     2581 gacgaggtga aggctctccg caacacgcgg ctcctcgagg tgacgggccg cgtggccgcg
     2641 gcgcggagcc tgcggctcga gggccggacg ctgccggtgc tggtcgacgg cgtctcgcgc
     2701 aagaatgcgg gggaggccgc gggccggacc cgctgcaatc gagtggtgaa cttcgatacc
     2761 ggaggccgcg acctgctggg ccgggtggtg gaggtgcgca tcgcccgggc cctgccgcac
     2821 agccttcgtg gtgagctggt cgacgcctcc ggggacccgg gcgcgatgga ccggctcccg
     2881 gtgggcctgg ccgagcccgc gttaccggcc taggaggagg cgcaccatgg tgctggagat
     2941 gaaagtgaag gggctcgcgc tggatccgct ctccaacatg cccatgatca tcctccgcga
     3001 cgaggaggag aagcgctcgc tgccgatctg ggtgggtctc ttcgaggcca acgccatcgc
     3061 gctggagctg gagaagatct ccacgccgcg gccgatgacc cacgacctca tcaagaacat
     3121 cctcgaggcc ctcgacgccc gcgtgcagaa ggtcgtggtg aacgacctcc gggacaacac
     3181 cttctacgcg gtgctctacc tccggctcgg caacgccgac atcacggtcg actcgcgccc
     3241 ctcggacgcc atcgcgctcg ccctgcgggt cggggcgccg atcttcgtgg aggaggacgt
     3301 ggtggacaag gcccgcaccg tcgaggtcgc caaggagacc gagatcggga ccgggaaggg
     3361 cgacgaccag gccaagatca aggaatggct cgagtcgctc aagccgggcg acttcgaccg
     3421 gctcgagcgc gacaaggacc cgaagcgccc ggacgaatag tggatgcgcg tcgggctgtt
     3481 caccaacaac tacctgccgt tccgcggggg agtcacgacc gcggtcgaga ccctgcgcct
     3541 cgggcttgaa gggctcggcc accgggcctg ggtcttcgcg ccggccgccc agcatccgca
     3601 tcaggatccc ccgttcgtct tccgctaccc gtcgatcccg gcgccgacct accccggttt
     3661 ctcgctgccg ctgccggtct cgcggcgcct gggtcggctc gcccgctcgc tcaacctcga
     3721 cgtcgtccac gtccaccacc cgttcctgct cggggtgacc ggtcggcggc tcgcccgcgg
     3781 ccaacggcgc ccgctcgtct tcacctacca cacgcgctac gagaagtacg cccactacgt
     3841 cccgctgccg ccgcgtctcg tccggagcct cgcggtgcgc ctcgcctgcc gcttcgcgga
     3901 ctcgacggac ctggtcgtcg cgccctccga tcacgtcgcg gacacgctgc gacgccgagg
     3961 cgtgcgcgcg ccggtcgcgg tgatccccac cggtgtcgat ctctcgctgt tcagcccggg
     4021 cagccgggag cgggcgcgtc gccggctcgg cctgcccgcg gaggggctca tctgcctcta
     4081 caccggccgg ctcgaccgcg agaagagcct cgagcgtgtg ctcgacgcct tcgagtcggt
     4141 ggcggccgcg ctgtccggcg cgaccctgca cctggtcggg aaggggagcc acggggcggc
     4201 gctcgaggcg cgcgcggcgg cgggccgcgc gggccgccgc gtcgtcttcc acgggggcct
     4261 cgcgcgggag gcgctgcccg actactatcg cgcggccgat ctcttcctct tcgcgtcgga
     4321 gaccgagacc cagggcctgg tgctggccga ggcgcacgcc tgcgcgctgc ccgcggtggc
     4381 ggtccgcgca tccggggtgg acgaggtggt gaccgacggg gagaccgggt tcctcaccaa
     4441 gggcgagacc ggcgagatgg ccgacgccgc gatcggtctc ctcctcgacg tggagcgccg
     4501 ggtggcgatg gggcaggccg cgcgccggct cgcggaggcg cgcttctcca ccgggcggca
     4561 gatcgacgcg atggtcgggc actacgaggc cctgatcgcg gggcggcggt gatgccgggc
     4621 tcgacgctcg gacgcacgct gcgggtgggc ctccagaccg actgggaccc gcataccgcc
     4681 ggccccgggc accaggggct gctggcctgg gaccagcggg tccgcaccct gctgcgcgtg
     4741 ctggtgtcct accccgaggt gcgctacatc ctgccggacc ggatcagcct cgaggcctcc
     4801 gccgacccgc gaacgctcga gaccatggcg cgcttcctcg agcgacagag ctggctcgtg
     4861 cgccgggtgg cggtgacgtg agcgccacga gtgggggccg cgcgtgatct ggaaccgcga
     4921 cgtcgagacc gcgccgcgcg cggccctgcg cgcgctgcag gccgagcggc tgcgcgccac
     4981 cgtggcccgc gcgcgggcgc gggtgccctt ctacaaggac gccttcgcgg aggccggcgt
     5041 gcgcgacggc gacgtcgacc tcgagcggct cgccgagctg ccgttcaccc gcaaggcgca
     5101 cctgcgtgac cactacccgt tcgggctctt cgcggtgccc cgcgaggagc tggtgcgcat
     5161 ccacgcctcc tcgggcacgc gcggcaagcc caccgtggtc ggctacacgc gcggcgacct
     5221 cgagctgtgg cgcgaggtga tggcccgcgc gctcgccgcc gccggcgccg agcccggcca
     5281 gctcgtgcag atcgcctacg gctacggcct gttcaccggc ggcctcggct tccacgacgg
     5341 ggccgagcac atggggctca cggtggtgcc ggtctcctcc ggcaacaccc tgcgccagat
     5401 cctgctcctc cagga
//
LOCUS       DATMCV010000757         2303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_704796, whole genome shotgun sequence.
ACCESSION   DATMCV010000757 DATMCV010000000
VERSION     DATMCV010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704796"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VNU03_17385"
     CDS             complement(<1..339)
                     /locus_tag="VNU03_17385"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWP76299.1"
                     /translation="MRFLANRGPIIAVALLVVALVADAVLSLHNIREVATSVQWVSHT
                     NEVLSRLEAVLSTLKDAETGQRGYLLTGEPAYLEPYRDALDRLPRQIADLRQLTLDNP
                     PQTVRVLRLDQ"
     gene            complement(358..1371)
                     /locus_tag="VNU03_17390"
     CDS             complement(358..1371)
                     /locus_tag="VNU03_17390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP76300.1"
                     /translation="MPRASFGLILANRAVVLGAIQARDLIELSQQAEASGAFDTLWVG
                     DSLLAKPRLEAVALLSALAGATSRMRLAVGCMATFVHRHPLLLAQQWASLDVLSGGRA
                     LLAVCLGGPDEQSAAQALEHRVMGIRSSERVGRMEEGIQILRKVFSGEKVSHQGEFYQ
                     FEGVKIEPRPVQQPCPIWIASNPTGLTWKGGASASDAAVERGFRRVARFADGWMTNKV
                     TPAQFREQWARIARMTREEGRNPDALGSALYHNININEDRQQALEESKTFLDTYYTSK
                     FSPAFVEGWTVAGSPAQCIAELREYVAAGLTHVSLRLTSWDQRGQLKRFLAEVAPALT
                     GGR"
     gene            1599..>2303
                     /locus_tag="VNU03_17395"
     CDS             1599..>2303
                     /locus_tag="VNU03_17395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWP76301.1"
                     /translation="MSLGAALTQAVQFAVVKGRAREIPPLVIVAWTQGVACAAWVVFF
                     VASAHPFTAPGALWPVVAVSCLLVLGMSGLLARASARGDISIVGPILALSPIFTILPD
                     AALSGGLPSPLGWIGLALSLTGTVSLSGGAGGRLGRIRTLFARRDALDALGAALLLGF
                     LAAVDRYGALHLGPPSYLVCSHGATALLAALIAAVTTPRGLAESVRPRNLLTVVIHGL
                     LGVTGTGMQTTALTMAS"
BASE COUNT          282 a          822 c          849 g          350 t
ORIGIN      
        1 ctgatcgagc cgcagcacgc gcacggtctg gggcggatta tcgagggtga gctgtcggag
       61 gtcggcgatc tggcgcggta ggcgatccag ggcgtcgcgg tatggctcca gataggccgg
      121 ctccccggtg agcaggtagc cgcgctggcc ggtctcggcg tccttgaggg tcgagaggac
      181 cgcctcgagc cggctcagga cctcgttggt gtgcgagacc cactgcacgc tggtggcgac
      241 ctcgcggatg ttgtgcagcg acagcaccgc gtccgcgacc agggccacca cgagcagggc
      301 gaccgcgatg atggggccgc ggttcgccag gaagcgcatg cctggcgcgg gcacctctca
      361 gcgcccgccc gtcagcgcgg gggcgacctc ggccaggaag cgcttgagct ggccgcgctg
      421 gtcccaggac gtgaggcgga gcgagacgtg ggtgagcccc gccgccacgt actcgcgcag
      481 ctccgcgatg cactgggccg ggctgcccgc gaccgtccag ccctcgacga aggcggggga
      541 gaacttggag gtgtagtagg tatcgaggaa ggtcttgctc tcctcgagcg cctgctggcg
      601 atcctcgttg atgttgatat tgtggtagag cgcgctgccg agcgcgtccg ggttgcggcc
      661 ctcctcgcgc gtcatccggg cgatgcgcgc ccactgctcg cgaaactggg ccggggtcac
      721 cttgttggtc atccagccgt ccgcgaagcg ggcgacccgc cggaagccgc gctccaccgc
      781 cgcgtccgac gcgctggccc cgcccttcca ggtgaggccg gtcgggttgc tcgcgatcca
      841 gatcgggcac ggctgctgga ccgggcgcgg ctcgatcttc acgccctcga actggtagaa
      901 ctcgccctgg tgggagacct tctccccgct gaagaccttc cggaggatct ggatcccctc
      961 ctccatgcgc cccacccgct cgctcgagcg gatgcccatc acccggtgct cgagcgcctg
     1021 cgcggcgctc tgctcgtcgg ggccgcccag gcagaccgcg aggagggccc ggccgccgga
     1081 gagcacgtcg aggctcgccc actgctgggc gagcaggagc gggtggcggt ggacgaaggt
     1141 ggccatgcag cccaccgcga gccgcatccg cgaggtggcg cccgcgaggg ccgagagcag
     1201 cgcgaccgcc tcgaggcgtg gcttggcgag caggctgtcg ccgacccaca gcgtgtcgaa
     1261 ggcgccggag gcctcggcct gctgcgacag ctcgatgaga tcgcgggcct ggatcgcgcc
     1321 gagcacgacc gcccgattgg ccaggatgag cccgaaggat gcgcgtggca tggatggcct
     1381 cccggcagca atcttggcgg ccggggcacg ggacgtcaag ggcgcggtcg atttgacctg
     1441 gcgtcgacac ctcgggtacg gtctcccggg aggagccgac cgatgagcgt gagccgcgca
     1501 ttccacgggg tcatccccgc gatggccatt ccgttccgcg acgccgcgct cgaggcgagc
     1561 ggggtgcccc gcgtcgagct cgtctgacct ggttcctgct gtcgctcggg gcggccctca
     1621 cccaggccgt ccagttcgcg gtcgtcaagg ggcgcgcccg ggagatcccg ccgctggtga
     1681 tcgtcgcgtg gacccagggc gtggcctgcg cggcgtgggt ggtgttcttc gtggcctcgg
     1741 cccatccgtt caccgcgccg ggggcgcttt ggcccgtggt ggccgtctcg tgcctgctcg
     1801 tgctcggcat gagcgggctg ctcgcccggg ccagcgcgcg cggagacatc tcgatcgtcg
     1861 ggcccatcct cgcgctgagc ccgatcttca cgatcctgcc ggacgccgcg ctctcgggcg
     1921 ggctgccgag cccgctcggc tggatcggcc tcgcgctctc cctcaccggt accgtcagcc
     1981 tgtcgggcgg agcgggcggg cgcctcggcc ggatccgcac gctcttcgcg cggcgagacg
     2041 cgctcgacgc cctgggcgcc gccttgctgc tcggctttct cgccgcggtg gaccgctacg
     2101 gggcgctcca cctcgggccg ccgagctacc tggtctgctc gcacggcgcg accgcgctcc
     2161 tggccgcgct gatcgcggcg gtcaccaccc cgcgcggtct ggccgagagc gtgcggcccc
     2221 gcaacctgct cacggtggtg atccacggtc tgctcggggt gaccggcacc ggcatgcaga
     2281 ccaccgcgct caccatggcc tcc
//
LOCUS       DATMCV010000758         3966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_909972, whole genome shotgun sequence.
ACCESSION   DATMCV010000758 DATMCV010000000
VERSION     DATMCV010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909972"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VNU03_17400"
     CDS             complement(<1..514)
                     /locus_tag="VNU03_17400"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP76302.1"
                     /translation="MDLGPSARQRELIALARDLARERFAPRAAQHDREASFPFDDYAD
                     LRSAGLLGLCVPARHGGLGADYETYCLVAEQLAQGNASTSLTFNMHCLTMLMMGVLAD
                     AMSMSSAARERHEKLRAAKFREVIEGGAFYGQPHSEPVEQGQTDTALSMGGRRFGTTA
                     RKVDGGYVVNG"
     gene            complement(561..1301)
                     /locus_tag="VNU03_17405"
     CDS             complement(561..1301)
                     /locus_tag="VNU03_17405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWP76303.1"
                     /translation="MTETAIRFRDDPWPRLRWLVPAALGLTLLSQMAFLSLLRQPASL
                     PTVPRPVDVHVIELPAPTPPAAAPPAPPERRSPPPRRLDAAPPRPVPAPSEPRIEVPR
                     APVPSVAEEPVTPAPAVTVPVPPAPAPPTTAPATQVPLTALPPSRGPQDATPGGTDRM
                     SARAIYKPIPEIPEALRRRTIDLVAVARFRVDATGRAQVELVEPTPDPDLNRALLESL
                     KRWRFFPATQAGRPIASTIDVRIPVSVK"
     gene            complement(1298..1696)
                     /locus_tag="VNU03_17410"
     CDS             complement(1298..1696)
                     /locus_tag="VNU03_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWP76304.1"
                     /translation="MKFRPSRQRRRGRIEIIPMIDVMFFLLATFMLASLSLQNLHSLT
                     VNLPRGQAPPLQAARSVTLTVTREGELLLDRTVVTLDTIAAELRPRLAAAGASVVVAV
                     DSAAPSGVVVQAMLKAREAGVEHFLFAVTP"
     gene            complement(1693..2328)
                     /locus_tag="VNU03_17415"
     CDS             complement(1693..2328)
                     /locus_tag="VNU03_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWP76305.1"
                     /translation="MDLSGFVAALRVGGAMIYPLLLLAVIAVVVILEKAFVFSTRTRL
                     PAPVLEVLETYAFAWEDLEQRVGQMDPRNYFRRFFRVILDQRARPTWWVESRAAEEAT
                     LIEQALGRWLWALETIVTAAPLLGLLGTITGMIRAFHLFGSEGLVDPRGVTGGVAEAL
                     IATAVGLFIALIALFAFNYFSNRQAQVMDEMEQLGTRVVDHLRLEATDGAP"
     gene            complement(2328..>3966)
                     /locus_tag="VNU03_17420"
     CDS             complement(2328..>3966)
                     /locus_tag="VNU03_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWP76306.1"
                     /translation="KTGAFEPGGHVGVYGGSHAWLQPSAEYRGAVGRFNYFLTGDYLQ
                     NSIGISPATPNGAIHDDTQQGHGFGYFEYLLDATSKVSAIAGSFVGHFQIPNRPNVSP
                     SFTVNGISEFDSRKIDETQREQNHFLVLSYLKTAGDFSVQASTFARYSTMSFRPDSLA
                     DLLFNGIAQQVDRSSIATGLQVDSTYTLTPTHTLHGGVYLAAERTSVQATSSVLPAVD
                     GVPTSDQPFKIFDSRGKTGYTYSVYLQDAWRILPTVTINGGLRLDGLEAFTSEWQLSP
                     RLNVVWEATSTTTIHAGYARYFTPPRQEFVATGSVARFANTTAEASVPVNAPVRAERA
                     HYIDAGVTQQIIPGLKVGLDAYYKQSLYHLDEGQFGAPTFLTPFNYHHATNIGIELTT
                     SYTAGGFSAYGNLAAAQQVAKGIVSAQALFDAEDLAYIHQHYIVTDHSQLITASAGLS
                     YLWRQTRVSLDLLVGSGLRRTVRHPNDATNPPYQQLDFGIAQRFTLPAIGAMEARFDV
                     INVLGQNYVLRDGTGVGIFARQFGPPRGFFAGVKKEF"
BASE COUNT          589 a         1238 c         1564 g          575 t
ORIGIN      
        1 ggccgttgac cacgtagccc ccgtccacct ttcgcgcagt ggtgccgaag cgccgcccgc
       61 ccatgctgag cgcggtatcg gtctggccct gctcgaccgg ctcgctgtgg ggctgcccgt
      121 agaaggcgcc cccctcgatc acctcgcgga acttggccgc gcggagcttc tcgtgacgct
      181 cgcgcgcggc ggaggacatg gacatggcgt cggcgagcac gcccatcatc agcatggtga
      241 ggcagtgcat gttgaaggtg agcgaggtcg acgcgttgcc ctgcgcgagc tgctccgcga
      301 cgaggcagta ggtctcgtag tccgctccga gcccgccgtg gcgggcggga acgcagaggc
      361 cgaggaggcc ggcggatcgg agatccgcat agtcgtcgaa ggggaaggac gcctcgcgat
      421 cgtgctgggc cgcgcgggga gcgaagcgct cgcgggccag atcgcgggcg agggcgatca
      481 gctcgcgctg gcgggcggac gggccgagat ccatggcccg acattgtgtc catcatcggg
      541 gccgcgagcg caagccccgg ctatttcacc gagaccggga tgcggacgtc gatggtggag
      601 gcgataggcc ggccggcctg ggtggcgggg aagaagcgcc atcgcttcag ggattcgagg
      661 agcgcgcggt tgagatccgg atcgggggtg ggctcgacca gctcgacctg ggcgcgaccc
      721 gtggcgtcca cccggaaccg cgccaccgcc accagatcga tcgtgcgccg gcgcagcgct
      781 tcggggatct cgggtatcgg cttgtagatg gcccgggcgc tcatgcggtc ggttccgccc
      841 ggggtggcgt cctggggccc gcgcgagggc ggcagcgcgg tcagcgggac ctgcgtcgcc
      901 ggcgcggtgg tcggcggcgc gggcgccgga ggcaccggca ccgtcacggc cggggccggt
      961 gtcaccggct cctccgcgac ggacggcacc ggcgcgcgcg gcacctcgat gcggggctcg
     1021 ctcggcgcgg gcaccggacg gggaggggcc gcatccaggc gcctcggcgg cggcgatctc
     1081 cgctccggtg gcgccggtgg ggccgcggcc ggcggtgtcg gggcgggcag ctcgatgacg
     1141 tggacgtcca ccggtctcgg gacggtgggc aggctcgcgg gctggcggag cagggacaga
     1201 aaggccatct gcgacaggag ggtgagaccg agcgccgccg gcaccagcca ccgcagccgt
     1261 ggccaggggt cgtcgcggaa ccggatcgcg gtctcggtca cggcgtgacc gcgaagagga
     1321 agtgctcgac gccggcctcc cgggccttga gcatcgcctg gacgacgacg ccgctcgggg
     1381 cggcactgtc caccgcgacg acgacgctcg cgcccgcggc ggcgagccgg ggtcggagct
     1441 cggccgcgat ggtgtcgagg gtgaccacgg tgcggtcgag caggagctcg ccctcacggg
     1501 tcacggtcag ggtgaccgat cgcgcggcct gcagcggggg cgcctggccg cgaggcagat
     1561 tcacggtcag cgagtgcagg ttctggagcg agagggaggc caacatgaag gtggcgagga
     1621 ggaagaacat gacgtcgatc atcgggatga tctcgatccg gccccggcga cgctggcgcg
     1681 acgggcggaa tttcacggcg cgccgtccgt cgcctcgagg cggaggtggt ccaccacccg
     1741 cgtgccgagc tgctccatct cgtccatgac ctgggcctgc cggttcgaga agtagttgaa
     1801 ggcgaagagc gcgatgaggg cgatgaagag ccccaccgcg gtggcgatga gggcctcggc
     1861 gacgcccccg gtgacgccgc ggggatcgac cagcccctcg gagccgaaga gatggaacgc
     1921 gcggatcatc ccggtgatgg tgccgagcag gcccagcagc ggagcggcgg tgacgatcgt
     1981 ctcgagggcc cagagccagc ggcccagcgc ctgctcgatc agggtggcct cctcggcggc
     2041 ccgcgactcg acccaccacg tcgggcgcgc gcgctggtcc aggatcacgc ggaagaatcg
     2101 ccggaagtag ttgcgggggt ccatctggcc gacgcgctgc tcgagatcct cccaggcgaa
     2161 ggcgtaggtc tcgaggacct cgaggaccgg ggcgggcagg cgagtgcggg tcgagaagac
     2221 gaaggccttc tcgaggatca ccaccaccgc gatgaccgcg agcagcagga gagggtagat
     2281 catggcgccg cctacccgca gggcggcgac gaagcccgag agatccatca gaactccttc
     2341 ttcacgcccg cgaagaatcc gcggggtggt ccgaactgcc gggcgaagat cccgaccccg
     2401 gtgccgtcgc gcagcacgta gttctggccg agcacgttga tcacgtcgaa gcgcgcctcc
     2461 atggccccga tcgcgggcag ggtgaagcgc tgggcgatgc cgaagtcgag ctgctggtag
     2521 ggcgggttgg tggcgtcgtt cgggtggcgc acggtgcgcc gcagtccgct gcccaccaag
     2581 aggtcgaggc tcacccgggt ctggcgccag aggtaggaga gcccggccga ggcggtgatg
     2641 agctggctgt ggtcggtgac gatgtagtgc tggtggatgt aggcgaggtc ctccgcgtcg
     2701 aacagggcct gggcggagac gatgcccttg gccacctgct gcgcggcggc caggttgccg
     2761 taggcggaga acccgcccgc ggtgtaggac gtcgtgagct cgatgccgat gttggtcgcg
     2821 tggtggtagt tgaacggggt caggaacgtg ggcgcgccga actgcccctc gtccaggtgg
     2881 tagagcgact gcttgtagta ggcgtcgagc cccaccttga ggccggggat gatctgctgg
     2941 gtgactcccg cgtcgatata gtgagcccgt tccgcccgca ccggcgcgtt gaccgggacc
     3001 gacgcctccg cggtggtgtt ggcgaaccgc gccacgctcc cggtcgcgac gaactcctga
     3061 cgaggcgggg tgaagtagcg cgcgtagccc gcgtggatgg tggtggtcga cgtcgcctcc
     3121 cacaccacgt tcagccgggg gctgagctgc cactcgctcg tgaaggcctc gagcccgtcg
     3181 aggcggagcc cgccgttgat ggtgacggtg ggcaggatgc gccacgcatc ctgaagatag
     3241 acgctgtagg tgtagccggt cttgccccgg ctgtcgaaga tcttgaacgg ctggtcggag
     3301 gtcggcaccc cgtcgacggc gggcagcacc gacgaggtcg cctgcacgct ggtgcgctcc
     3361 gccgcgaggt agaccccccc gtgcagggtg tgggtcgggg tgagggtgta ggtggagtcg
     3421 acctgcaggc cggtcgcgat gctgctccgg tccacctgct gggcgatgcc gttgaacagc
     3481 aggtcggcga gcgagtcggg gcggaaggac atcgtgctgt agcgggcgaa ggtggaggcc
     3541 tgcacgctga agtcgcccgc ggtcttgagg taggacagga ccaggaagtg gttctgctcc
     3601 cgctgggtct cgtcgatctt ccgggagtcg aattccgaga tgccgttgac cgtgaagctg
     3661 ggcgagacgt tgggccggtt cgggatctgg aagtggccga cgaagctccc ggcgatggcg
     3721 ctgaccttgc tggtcgcgtc gaggagatac tcgaagtatc cgaagccgtg gccctgctgg
     3781 gtgtcgtcgt ggatcgcgcc gttgggggtg gccggggaga tgccgatgct gttctggagg
     3841 tagtcgccgg tcaggaagta gttgaagcgg ccgacggccc cgcgatactc cgcgctgggc
     3901 tggagccacg cgtggctgcc gccgtagacg cccacgtgcc cgcccggctc gaaggcgccg
     3961 gtcttg
//
LOCUS       DATMCV010000759         4671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_639451, whole genome shotgun sequence.
ACCESSION   DATMCV010000759 DATMCV010000000
VERSION     DATMCV010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639451"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..930)
                     /locus_tag="VNU03_17425"
     CDS             complement(136..930)
                     /locus_tag="VNU03_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP76307.1"
                     /translation="MDLGLRGKTALVTGGSKGIGRAVARTLAAEGARVVICSRDAGAL
                     KDAAGDIERATGGRVETVAGDLSRHDEVQRVAAEAVARLGRLDILVNNAGAIKGGDFL
                     TTPDEEWLKGWSLKLLGYIRMAREVLPHMQRQAGGRIVNVVGAAARNPAVTYMMGGTA
                     NAALINFTKALADLGAPSGVLVTGVSPGPVKTERWDSLMAQQAKAANQNLDDYVKERG
                     QELPLGRIALPEEVADVVCFLASERASFLTGITITVDGGITRGVYL"
     gene            complement(948..1484)
                     /locus_tag="VNU03_17430"
     CDS             complement(948..1484)
                     /locus_tag="VNU03_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76308.1"
                     /translation="MHLITDTAAVAGRRKLVPPGRLIEAWPDLYVAGQAWVGDETRAL
                     LDGAGDAMPVALSIEDTAVPIYYGPRLQDTESLPLEASLRARVLSAHGIAAAWITVDQ
                     FGERTVYEPTGPADPIFFLRRPGGSAAHIWRLFRSRAEARVYMAEYYGRDSEGRDWAE
                     TLSVETFEDLLARHARRS"
     gene            complement(1500..2147)
                     /locus_tag="VNU03_17435"
     CDS             complement(1500..2147)
                     /locus_tag="VNU03_17435"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HWP76309.1"
                     /translation="MTLPLPLRNRLADLVLDALHDREARRALEALAAVCADPRALDAA
                     PPPVAFPADLFDRGPEGWRIKSGYRQHAAPFAERAGRAARALADRPLAPADPPLETAL
                     DHAATLFAAGLYFEVHEVLEPHWMRAEGPARQTLQGLIQAAVGYQHLANGNLEGARAL
                     LHDGAAKLLGRRLAGRDLDAFARAVAGHSRELAGGDAAAAGRFDWTRVPPFPAEA"
     gene            complement(2144..2662)
                     /locus_tag="VNU03_17440"
     CDS             complement(2144..2662)
                     /locus_tag="VNU03_17440"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP76310.1"
                     /translation="MAATRCPMCRIAHTRLGSVRPLRALRSAPGRLARAVRRVPRRLV
                     ARRPARGEWAVAEVMSHLADAEIALAFRIRKIAAEPRSVLVAWDQDRWAEGGRYRRTP
                     PGEALATYTALRRSNLAYVGRLGAAARRQHGRHPEYGRLTMAQLLDHWAEHDLIHLEQ
                     IQAAHRRLAGRA"
     gene            2774..3976
                     /gene="glp"
                     /locus_tag="VNU03_17445"
     CDS             2774..3976
                     /gene="glp"
                     /locus_tag="VNU03_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWP76311.1"
                     /translation="MISVTDAQAHVLSLVARVAAPEVIPVTAGLGRVLAEDVRATLDV
                     PPTANSAVDGYAVRGGDIPTAGTRDLTVVADLAAGAVFEATIGPGEALRIMTGAPIPA
                     GADTVYPQELVERAGRVVRIPAGPVGVNVRQRGEDVRAGTVVLPAGVALRPQELGVAA
                     SLGLPQLLVRQKPRVAILSTGDEVTEPGHARKPGQIFDSNRFSLHGLVEAAGGEPTDH
                     GIVPDLYDVLHARLRAAATTADVVLTSGGVSVGDHDLVKAVLQDAGGIDFWQVAMQPG
                     RPLAVGRIGPALFFGLPGNPVASMLTFHLFVRPALWKLGGRRDLFPPRFHAIAMEPMS
                     KKTGRREFKRGILTYAGDRWEVRTTGPQGSGILTSMTLANCFVVIDEERGDVAEGETV
                     SVEPFAVS"
     gene            3984..>4671
                     /locus_tag="VNU03_17450"
     CDS             3984..>4671
                     /locus_tag="VNU03_17450"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HWP76312.1"
                     /translation="MPHGIETRLIEGITPMVRDLVTGYARSLPGLRYADVRVEVSEGK
                     FAGAENGEAKSSGDDYAFAFGIRVLAGTRMSAPGYFGRGLGAADLPELARLLREGLQA
                     AYRRAMANAEMKAEVKAKFGSLGDALEDTRLHPIPVRQEIVPAVFEVDPRAVALDEMV
                     WFTTDVSRQVAAADAAVSYNYVSTLTQLSRELFASSEGALIDQSFALTQGMAYVVATG
                     PDNSQEIYDVL"
BASE COUNT          615 a         1694 c         1710 g          652 t
ORIGIN      
        1 acgtagtcag gcccgcagcg tgcccgctac cctagtcgtg gtctaaccgg ctgtcaaggg
       61 aatattcctg cagaatgttc tttgtttcac gaggttatgt ccttactggg tgtgcaatcc
      121 atgcggctgt aacggctaga gatacactcc gcgcgtgatg ccgccatcga ccgtgatggt
      181 gatcccggtc aagaatgaag cgcgctcaga cgcgagaaag cagaccacgt ctgcaacttc
      241 ttccggaagc gcgatgcggc cgaggggcaa ttcttgacca cgttctttta cgtagtcgtc
      301 gagattctgg ttcgcggcct tcgcctgctg ggccatgagg ctgtcccacc gctcggtctt
      361 gaccggaccc ggcgaaacgc cggtcacgag cactccagag ggtgcgccga gatcggcgag
      421 ggccttggtg aagttgatga gcgcggcgtt cgcggtgccg cccatcatgt aggtgaccgc
      481 cgggttgcgc gccgccgcgc cgacgacgtt cacgatgcga ccgcccgcct gccgctgcat
      541 gtgcggcagt acctcgcggg ccatccggat gtagcccagc agcttcaggc tccagccctt
      601 gagccactcc tcgtcgggcg tggtgagaaa gtcaccgccc ttgatcgcgc ccgcattgtt
      661 caccaggatg tcgagccggc ccaggcgggc gacggcttcg gcggccacgc gctgcacctc
      721 gtcgtgccgg ctcaggtcgc ccgccaccgt ctcgacgcgc ccgccggtgg cgcgctcgat
      781 gtcgccggcc gcgtccttga gcgcccctgc gtcgcgcgag cagatgacca cccgcgcgcc
      841 ttccgccgcg agcgtgcgcg ccaccgcacg gccgatgccc ttgctcccgc cggtcacgag
      901 cgcggtcttg ccccgaagtc ccagatccat gatgtcgctc ctctcggtca gctccgccgg
      961 gcgtgacgcg ccagcagatc ctcgaaggtc tccacggaca gcgtctcggc ccagtcgcgc
     1021 ccctccgagt cgcggccgta gtactcggcc atgtagacgc gcgcctccgc ccggctgcgg
     1081 aagagccgcc agatgtgcgc cgcgctcccg ccggggcggc gcaggaagaa gatcggatcg
     1141 gccggcccgg tcggctcgta gacggtgcgc tccccgaact gatcgacggt gatccacgcc
     1201 gccgcgatgc cgtgggccga gagcacgcgc gcgcgcagcg acgcctcgag gggcagcgac
     1261 tcggtatctt gaagtcgcgg tccgtagtag atcggcaccg cggtgtcctc gatcgagagc
     1321 gcgaccggca tcgcgtcccc cgccccatcg agcagggccc gcgtctcgtc gccgacccac
     1381 gcctgcccgg ccacgtagag atcgggccac gcctcgataa gacggcccgg cggcacgagc
     1441 ttgcggcgcc ccgccaccgc cgcggtgtcc gtgatcagat gcacgcgggc cactcgcggt
     1501 caggcctccg cgggaaacgg agggacccgc gtccagtcga accgccccgc ggcggctgcg
     1561 tcgccgccgg cgagctcgcg ggagtggccg gccaccgcgc gcgcgaaggc gtcgaggtcg
     1621 cgcccggcga ggcgccgtcc gagcagcttc gcggcgccgt cgtgcagcag ggcgcgcgcg
     1681 ccctcgaggt tgccgttggc caggtgctgg tagcccaccg cggcctggat gaggccctgg
     1741 agcgtctggc gcgcggggcc ctcggcgcgc atccagtggg gctcgagcac ctcgtggacc
     1801 tcgaagtaga ggccggccgc gaagagggtc gcggcgtggt cgagggcggt ctcgagcggc
     1861 gggtccgcgg gcgccagcgg acgatccgcg agggcccggg ccgcgcggcc cgcgcgctcc
     1921 gcgaagggcg ccgcgtgctg ccggtaaccc gacttgatgc gccagccttc cggcccgcga
     1981 tcgaagaggt cggccgggaa cgcgaccggc ggcggggccg cgtccagcgc gcgcgggtcc
     2041 gcgcacaccg cggcgagcgc ctcgagagcg cggcgcgcct cgcggtcgtg cagcgcgtcg
     2101 aggacgagat cggccaggcg attgcgcagc ggcagcggca gcgtcacgct cgtccggcga
     2161 ggcgacggtg cgcggcctgg atctgctcga ggtgaatcag gtcgtgctcc gcccagtggt
     2221 cgagaagctg cgccatggtg agccggccgt actccggatg ccgtccgtgc tgccgccgcg
     2281 cggcggctcc gagccggccg acgtaggcga ggttggagcg ccgcagcgcg gtgtaggtcg
     2341 cgagcgcctc ccccggcggg gtgcgccgat agcggccgcc ctcggcccag cggtcctggt
     2401 cccacgcgac cagcaccgag cgcggctcgg ccgcgatctt gcggatgcgg aaggcgagcg
     2461 cgatctcggc gtcggccagg tggctcatca cctccgcgac cgcccactcc ccgcgcgccg
     2521 ggcggcgggc gacgagccgg cgcggcacgc gccggaccgc gcgggcgagc cggcccgggg
     2581 ccgagcggag cgcgcggagg ggacggacgg acccgagtcg cgtgtgggcg atgcggcaca
     2641 tcgggcaacg ggtggcggcc atggaggact ccctggactg acgggttcga cgcgcgagct
     2701 cggtgcgcaa gcccggcgcg tatcgtaaca cgctcgcgcg gggcgccgcc gggccgcgga
     2761 gtataatgcg cgcatgattt ccgtcaccga cgcgcaggcg cacgtgctct cgctggtggc
     2821 gcgcgtggcc gcgcccgagg tgatcccggt gacggccggg ctcgggcggg ttctcgcgga
     2881 ggacgtgcgc gccaccctcg acgtgccgcc caccgccaat tccgcggtcg acggctacgc
     2941 ggtccgcggg ggcgacatcc cgaccgcggg gacccgcgac ctgaccgtcg tggccgacct
     3001 ggccgcgggg gcggtgttcg aggctacgat cgggcccggc gaggcgctgc gcatcatgac
     3061 cggggcgccg atccccgcgg gcgcggacac cgtctatcca caggagctgg tggagcgcgc
     3121 gggtcgcgtg gtgcggattc ccgccggtcc ggtgggcgtc aacgtccgcc agcgcggcga
     3181 ggacgtgcgc gcgggcaccg tggtcctgcc cgccggggtc gccctccgcc cgcaggagct
     3241 cggggtggcc gcctcgctcg ggctgccgca gctcctggtg cgccagaagc cgcgggtggc
     3301 gatcctctcc accggcgacg aggtgaccga gccgggtcac gcgcggaagc cgggccagat
     3361 cttcgactcg aatcggttct cgctccacgg gctggtcgag gccgcgggcg gcgagccgac
     3421 cgaccacggc atcgtgcccg atctctacga cgtgctgcac gcgcgcctgc gcgccgcggc
     3481 caccaccgcc gacgtggtgc tcacctcggg cggcgtctcg gtgggcgacc acgacctggt
     3541 caaggcggtc ctgcaggacg cggggggcat cgacttctgg caggtggcga tgcagccggg
     3601 ccgcccgctc gcggtgggcc gcatcggccc cgcgctcttc ttcggcctgc ccggcaaccc
     3661 ggtggcgtcg atgctcacct tccacctctt cgtgcgcccg gccctctgga agctcggcgg
     3721 ccggcgcgac ctgttcccgc cgcggttcca cgcgatcgcg atggagccga tgagcaagaa
     3781 gacgggccgg cgcgagttca agcgcggcat cctcacctac gcgggcgacc gctgggaggt
     3841 gcgcaccacc ggcccgcagg gctcgggcat cctcacctcg atgacgctcg ccaactgctt
     3901 cgtggtgatc gacgaggagc ggggcgacgt ggccgagggc gagacggtgt cggtcgagcc
     3961 cttcgcggtg tcctgagcga gccatgcccc acggcatcga gacccggctg atcgaaggca
     4021 tcaccccgat ggttcgcgac ctggtcaccg ggtacgcccg ctcgctcccc ggcctgcgct
     4081 acgccgacgt ccgcgtcgag gtcagcgagg gcaagttcgc gggcgccgag aatggcgagg
     4141 ccaagtcctc cggcgacgac tacgccttcg ccttcgggat ccgggtcctg gccggcaccc
     4201 gcatgagcgc gcccggctac ttcggccgcg ggctcggcgc cgccgatctc ccggagctgg
     4261 cccgcctgct ccgcgagggc ctgcaggccg cctaccgccg ggcgatggcc aacgcggaga
     4321 tgaaggccga ggtgaaggcc aagttcggct cgctcggcga cgcgctcgag gacacgcgcc
     4381 tgcatccgat cccggtccgc caggagatcg tgcccgcggt cttcgaggtg gatccgcggg
     4441 cggtcgcgct cgacgagatg gtgtggttca ccaccgacgt gtcccgccag gtggccgccg
     4501 ccgatgccgc cgtctcctac aactacgtct ccactctcac ccagctgagc cgggagctgt
     4561 tcgcctcgtc ggagggcgcg ctcatcgatc agtcgttcgc gctcacccag ggcatggcct
     4621 acgtggtggc gaccggaccc gacaacagcc aggagatcta cgacgtgctt g
//
LOCUS       DATMCV010000760         2446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_976664, whole genome shotgun sequence.
ACCESSION   DATMCV010000760 DATMCV010000000
VERSION     DATMCV010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976664"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VNU03_17455"
     CDS             complement(<1..328)
                     /locus_tag="VNU03_17455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76313.1"
                     /translation="MVEQLLNALTLAGLLFLVSAGLSLVFGILRVVNFAHGVFYMLGA
                     YLGFTTVALSGAFWPALVAVPLVVGTLGALIESSTLRFIYRRPPIYQLLLTFGLALIL
                     EEGIRIL"
     gene            complement(330..1535)
                     /locus_tag="VNU03_17460"
     CDS             complement(330..1535)
                     /locus_tag="VNU03_17460"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76314.1"
                     /translation="MRRRDFLTMAAVGGVGAGVGVPALLRRAAAATPIKIGMPAALSG
                     GYAQYGIQAKRAAELFTKDITAKGGVLGRPVEFLYEDTAGDPATAVRKAQKLVEKDGV
                     KFLTGIALSSEALAVSAKCPEWKVIFMSTINGAGSLTAKAWNRYFFRVNTSGPMGARA
                     ISLYLAEGPMKRFFALGSDYAWGRDSVASFTSQITAAKKEIVGTDYPPVGTKDFASYI
                     AKIRQSRADGVYLALPGQDATIYLKQAHQFGLNREVKPIMEILELENMKAVGDAMVGA
                     IGSSRYPFTVDTPRNRDFVKRFHALHGLYPDMFDGETYEGLEWLAQVIQKAGTDDVEK
                     VIDAWEDSSYDGLEGPFFMRKCDHQAVQLGFAVEAVKDPKYPHPVPKILATYPGEKVT
                     PKCRSEDFS"
     gene            complement(1567..2358)
                     /locus_tag="VNU03_17465"
     CDS             complement(1567..2358)
                     /locus_tag="VNU03_17465"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP76315.1"
                     /translation="METGLRGKIAMISGASKGIGRAIAEKLAAEGASLSLCARSSEPL
                     RAVARQIEDKHGGACLAVPADLNKDADIRDWVAATIARFGGLDILVNNAGAAQGGAFL
                     DLPDQVWRDSLNLKLFGYIRVAREVFPHLVRRGGGRIVNVIGIGGVQPMDNYMVGGAA
                     NAALLNFTKALANEGAPHGILVTGVNPGATKTERWDTMLAKWGAAKGVTPGQAEREIL
                     DGVPLKRAGTVDEVANLVVFLASDLSSYITGTTVNVDGGMARMIF"
BASE COUNT          373 a          842 c          841 g          390 t
ORIGIN      
        1 agaggatgcg gatcccttcc tcgaggatga gggcgaggcc gaaggtcagc aggagctggt
       61 agatcggcgg acgacgatag atgaagcgca gcgtggagga ctcgatgagc gcccccagcg
      121 tgcccacgac cagcggcacc gccacgagcg ccggccagaa cgcgccggag agcgcgacgg
      181 tggtgaaccc gaggtaggcg ccgagcatgt agaagacgcc gtgcgcgaag ttgacgacgc
      241 gcaggatgcc gaacacgagc gagagcccgg ccgacaccag gaagaggagc cccgccagcg
      301 tcagcgcgtt gaggagctgc tcgaccacgc tacgagaagt cctcgctgcg gcacttcggg
      361 gtcaccttct cgcccgggta ggtcgcgagg atcttcggca ccgggtgcgg atacttcggg
      421 tccttcaccg cctcgaccgc aaagccgagc tggaccgcct ggtgatcgca cttgcgcatg
      481 aagaatggcc cctcgaggcc gtcgtagctc gagtcctccc aggcgtcgat caccttctcg
      541 acgtcgtcgg tgccggcctt ctggatcacc tgggccagcc actcgaggcc ctcgtacgtc
      601 tcgccgtcga acatgtcggg atagagacca tgcagagcgt ggaagcgctt gacgaagtcg
      661 cggttccgcg gcgtgtccac cgtgaacgga tagcgcgagc tgccgatcgc gcccaccatc
      721 gcgtcgccca ccgccttcat gttctcgagc tcgaggatct ccatgatcgg cttcacctcg
      781 cggttgaggc cgaactggtg ggcctgcttc aggtagatgg tggcgtcctg gcccggcagg
      841 gccaggtaga cgccgtcggc gcgggactgc ctgatctttg cgatgtagct cgcgaagtcc
      901 ttcgtgccga cgggcgggta gtcggtgccg acgatctcct tcttggccgc cgtgatctgc
      961 gatgtgaagg acgccacgct atcccgcccc caggcgtagt cactgcccag cgcgaagaag
     1021 cgcttcatgg ggccctcggc cagatacagg ctgatcgcgc gcgcgcccat gggcccgctc
     1081 gtgttcaccc ggaagaagta ccggttccag gccttcgcgg tgagggagcc cgcgccgttg
     1141 atggtggaca tgaagatgac cttccactcg gggcacttgg cggagacggc cagcgcctcg
     1201 gaagacagcg cgatgccggt gagaaacttc acgccgtcct tctccaccag cttctgggcc
     1261 ttgcgcaccg cggtggccgg atcgcccgcg gtgtcctcgt agaggaactc caccggtcgg
     1321 cccaggacgc cgcccttcgc cgtgatgtcc ttcgtaaaca gctcggccgc ccgcttggcc
     1381 tggatgccgt actgcgcgta gccgccggag agcgcggcgg gcatcccgat cttgatcggc
     1441 gtggccgccg ccgcgcggcg gaggagcgcc ggcacgccga cgcccgcgcc caccccgccc
     1501 accgccgcca tcgtcaggaa gtcgcgtcgc ctcatggtcg tcctcctcct tcgcggccgg
     1561 cccgcatcag aagatcatgc gggccatgcc gccgtccacg ttgaccgtcg tgccggtgat
     1621 gtacgatgac agatcggacg cgaggaatac gacgaggttg gccacctcgt ccacggtgcc
     1681 cgcgcgcttg agcgggacgc cgtcgagaat ctcgcgctcg gcctggccgg gggtcacgcc
     1741 cttggccgcg ccccacttgg cgagcatggt gtcccagcgc tcggtcttcg tcgcgccggg
     1801 attcacgccg gtgacgagaa tgccgtgggg cgcgccctcg ttggcgagcg ccttcgtgaa
     1861 gttcaggagc gcggcgttgg cggcgccgcc caccatgtag ttgtccatcg gctgcacgcc
     1921 gccgatgccg atgacgttga cgatgcgccc accaccccgc cgcacgagat gcggaaagac
     1981 ctcgcgcgcc acgcggatgt agccgaagag cttcaggttg aggctgtcgc gccagacctg
     2041 gtcgggcagg tcgaggaagg cgccgccctg cgcggcgccc gcgttgttga cgaggatgtc
     2101 gaggccgccg aagcgggcga tcgtggccgc gacccagtcc cggatgtccg cgtccttgtt
     2161 caggtcggcg ggcaccgcga ggcaggcgcc gccgtgcttg tcctcgatct gcctcgccac
     2221 cgcgcgcagc ggctcggagc tgcgcgcgca cagtgagagg ctcgcgccct cggcggcgag
     2281 cttctccgcg atcgcgcgtc cgatcccctt gctcgcgccc gagatcatgg cgatcttgcc
     2341 gcgcaggccg gtctccatcc gggcgcgata ttgctccggc gaggtgggcc tgtcaagcgg
     2401 cccgcccgct cccggtcggc gttgacaacc gtccggccgg agcgta
//
LOCUS       DATMCV010000761         2681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_107322, whole genome shotgun sequence.
ACCESSION   DATMCV010000761 DATMCV010000000
VERSION     DATMCV010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107322"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VNU03_17470"
     CDS             <1..254
                     /locus_tag="VNU03_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76316.1"
                     /translation="DRLRRRHTVILESEAARALADPSLRAAEALVATAAAWEQERATG
                     SRLAAARDEAERRLMARERDEAGRLARDYWATAERLLAR"
     gene            complement(264..638)
                     /locus_tag="VNU03_17475"
     CDS             complement(264..638)
                     /locus_tag="VNU03_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP76317.1"
                     /translation="MRTLRILVVDDQEHMRELLVEALSADGHTVDPAENGTAAIQLFE
                     AQAYDLVVSDLQMPQMDGPKLYEEVCKRWPAAAPGFVFITGEDEAPSYRQFLKDSKLP
                     VVAKPFKLKEFRQLLGTLFPPA"
     gene            724..2247
                     /locus_tag="VNU03_17480"
     CDS             724..2247
                     /locus_tag="VNU03_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HWP76318.1"
                     /translation="MLARVRSATLSGVEAATVFVEVDVTAGLPSFTTVGLPDSTVRES
                     RDRVRAAIHNVGLEFPIDRITVNLAPAELRKEGAAFDLPMAVGILCATGLVKPGRLDR
                     ALVMGELSLDGSVRAVRGVLPIALHARREGFAPLLVPAGNAAEASVVAGVEVIAIATL
                     HDAVEYLNGEREIAPARPDGGSGGPAAPDRDGLDFADVRGQAHAKRGLEIAAAGAHNV
                     LMIGPPGTGKTMLARRVASILPPLSLEEAIEASTVWSVAGLLSPGQGLLAVRPFRSPH
                     HTASEAGLIGGGGVPHPGEISLAHHGVLFLDELPEFSTRVLEALRQPLEDARVVVSRA
                     AGTVAFPARFQLVAAANPCRRGCPSLPSCACSPPERVRYLGRLSRPLLDRIDLHLEIP
                     ALSHAELTASAAGEPSAAIRARVVAARARQVARFAGTAIHANARMSGRQTRRICTLPP
                     DAARLLGLAVTRLGLSARAHDRLLRVARTIADLAAADAIATEHVAEAIQYRTLDRAR"
     gene            2373..>2681
                     /locus_tag="VNU03_17485"
     CDS             2373..>2681
                     /locus_tag="VNU03_17485"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWP76319.1"
                     /translation="MVLGREHTALVVIDVQERLFPAMDSDHREEVMRNLKVLAATAQR
                     LQLPTLATEQYPKGLGHTLPEMKAALPTGLEPIEKVAFSCWGVEAFRSRLTASGARQI
                     L"
BASE COUNT          345 a         1005 c          932 g          399 t
ORIGIN      
        1 tcgacaggct gaggcgccgc cacaccgtga ttctcgagag cgaggccgcc cgcgcgctgg
       61 ccgatccgtc cctgcgggcc gcggaggctc tcgtggccac cgcggctgcg tgggagcagg
      121 agcgtgcgac gggaagccgg ctcgcggcgg cgcgggacga ggcggagcgg cggctgatgg
      181 cccgggaacg tgatgaggcg ggacgtctgg cgcgcgacta ctgggcgacc gccgagcgac
      241 tgctcgctcg gtgaagaggg cgcctacgcg ggcgggaaga gcgtccccag cagctggcgg
      301 aactccttga gcttgaacgg cttggcgacc accggcagct tggagtcctt gaggaattgc
      361 cggtagctcg gcgcttcatc ctcgccggtg atgaagacga accccggcgc cgccgccggc
      421 cagcgcttgc agacctcctc gtagagcttg ggcccgtcca tctgcggcat ctgcaggtcg
      481 ctcaccacca ggtcgtaggc ctgggcctcg aatagctgga tcgccgccgt cccgttctcg
      541 gccggatcca cggtgtgccc gtccgccgag agggcctcca ccagcagctc gcgcatgtgc
      601 tcctgatcgt cgaccacgag gatgcgtagc gtccgcatgc cacgagtcta gcagagacgg
      661 ccgcccgggc gtatcggttc cgggacattg acgggatcgg cccggcattc ccacactggg
      721 cccgtgctcg cgcgcgtgcg ctccgcgacg ctctccggag tcgaggcggc caccgtcttc
      781 gtcgaggtgg acgtgactgc cggcttgccg tcattcacca cggtgggctt gcccgactcc
      841 acggtgcggg aaagccggga ccgcgtccgg gcggcgattc acaacgtggg cctcgagttc
      901 ccgatcgacc ggatcacggt gaatctcgcc ccggccgagc tccgcaagga gggcgccgcc
      961 ttcgacctgc cgatggcggt gggtatcctc tgcgccaccg gcctcgtgaa gccgggccgg
     1021 ctcgatcgcg cgctcgtgat gggcgagctg tcgctcgacg gcagcgtacg ggcggtgcgc
     1081 ggcgtcctcc cgatcgcgct gcacgcgcgc cgcgagggct tcgcgccgct gctcgtgccg
     1141 gccggcaacg cggccgaggc cagcgtggtc gcgggcgtcg aggtcatcgc catcgccacg
     1201 ctgcacgacg cggtcgagta cctcaacggc gagcgcgaga tcgctccggc gcgtccggac
     1261 ggcggctccg gcggcccggc cgcccccgac cgcgacgggc tcgacttcgc ggacgtgcgc
     1321 ggccaagcgc acgcgaagcg cggtctggag atcgcggcgg cgggcgcgca taacgtactc
     1381 atgatcggac cacccggcac cggcaagacg atgctggccc gccgcgtcgc ctccatcctg
     1441 ccgcccctct cgctggagga ggcgatcgag gcgtcgaccg tctggagcgt ggccggcttg
     1501 ctgtcgcccg gtcagggcct gctcgcggtg cgcccgtttc gcagcccgca ccatacggcc
     1561 tcggaggccg gcctcatcgg tggcggcggc gtcccccatc ccggcgagat cagcctggcg
     1621 caccacggcg tcctgttcct ggatgagctt ccggagttct ccacccgagt gctcgaggca
     1681 ctccggcagc cgctcgagga cgcgcgtgtc gtcgtctcgc gcgcggccgg caccgtagcc
     1741 ttcccggccc ggttccagct cgtggccgcc gccaacccct gccgccgtgg ctgtccgtca
     1801 ctcccgagct gcgcctgttc gccgcccgag cgcgtgcgct acctgggtcg cctctcccgg
     1861 cccttgctcg accgcatcga tctccacctc gagattcccg ccctgtccca cgccgagctc
     1921 accgccagcg ccgcgggcga gccgtctgcc gccatccggg cccgcgtcgt cgcggcgcgc
     1981 gcccgacagg tggcgcgctt cgcgggtacc gccatccacg ccaatgcccg gatgtcgggc
     2041 cgccagacgc gtcggatctg cacgctcccg cccgatgccg cgcgcctgct gggcctggcg
     2101 gtcacccgcc tgggcctctc cgcgcgcgcc cacgaccgtc tgcttcgcgt ggcccgcacc
     2161 atcgccgacc tggccgcagc cgacgccatc gccaccgagc acgtcgccga ggccatccag
     2221 taccgcacgc tcgatcgcgc gcgctgagat ccccgacggc ttgcctccac tcgcagatgt
     2281 gagaggatgg cggcggcttc agatcctcac ctccggaggt tccgatgacc cagcccggca
     2341 gccctttcgc gacggcatca gcgtcccatc ccatggtgct cgggcgcgag cacaccgctc
     2401 tggtcgtgat cgacgtgcag gagcggctgt tccctgcgat ggactcggat catcgcgagg
     2461 aagtgatgcg gaacctcaag gtcctcgccg cgacggccca gcgtctgcag ctgccgacct
     2521 tggcgaccga gcagtatccc aaggggctcg ggcacaccct gccggagatg aaggcggccc
     2581 tgcccaccgg gctcgagccg atcgagaagg tcgcgttctc gtgctggggg gtcgaggcgt
     2641 tccggagccg tctgaccgcg agcggcgccc gtcagatcct g
//
LOCUS       DATMCV010000762         2741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_773658, whole genome shotgun sequence.
ACCESSION   DATMCV010000762 DATMCV010000000
VERSION     DATMCV010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773658"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VNU03_17490"
     CDS             complement(<1..774)
                     /locus_tag="VNU03_17490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76320.1"
                     /translation="MSVLITPTMVGQVVISGLLAGSIYAMVALGLGLIFGVMRVLNVA
                     HGPLMMLGAYTTFWLFQRFGLNPYLSILVSMPVLFLIGVALQRFLVRRVVDAPELSSL
                     LLTFGVSIALVNLAQLAFTSDLRSVEYLTGSYVVGPFALSKPRLAACAFAIGITAASF
                     VFLRSTRLGKAIRAVSQSREVAQVCGIDVQRIHLLAFGLGSALAAAGGSLVAVMVAIQ
                     PEMGQVYTFKSFLVIVLGGAGNYPGALLGGLLLGLVEQLS"
     gene            complement(830..2041)
                     /locus_tag="VNU03_17495"
     CDS             complement(830..2041)
                     /locus_tag="VNU03_17495"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWP76321.1"
                     /translation="MIMTSFRYLGRCLGKRLAPVAIAGVVAASLPLPAGAQTPIKIGA
                     SMSVTGTYAKPGSYQKEGYDICIDELNGKGGLLGRKVELVIYDDQSQPATAVKLYEKL
                     ITEDKVDAVMGPYSSAVSEAVANVTEKYKKVMVAPLAATTSIFKKGRKYIFMVITPAE
                     NYLDGLIDMAAKRGLKTVAIINEDTLFPKASAAGTAEAAKKRGMQVVLQEAYPKGNTD
                     FSALLVKIKAANPDVIAAGTYFDDAVAITRQMKELNVNPKMFGLTVGGDLPEFYDLLK
                     QNAEYVYGSTQWDESLPYPGQKEFLAAYTKKFKHEPSYHAAAGYAGCLIYGEAVKRAG
                     TLDADRVREQLLKMEIKTAFGDYKVEPDGFQVAHKMVMLQWQDGKRVVVWPDDLASGK
                     LRYPTPEWGKR"
     gene            2197..2718
                     /locus_tag="VNU03_17500"
     CDS             2197..2718
                     /locus_tag="VNU03_17500"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP76322.1"
                     /translation="MAREIVRAVHHPDKRHVEMPYAPALLVRGGQLLFLSGLTAAPVY
                     HSHPHREEEFDLPPRMREQAVLLMENLKTTLAAAGCTLADVVSATRYLTDVAEQDDLN
                     AVWASYLGAHRPTTTTVEVSRLATHANCKIEISVIACFARRSQRRAERKQEELTRSSR
                     KTTRPGRKTARRK"
BASE COUNT          470 a          936 c          905 g          430 t
ORIGIN      
        1 ggagagctgc tcgaccagcc cgagcaggag cccacccagg agcgcgcccg ggtagttgcc
       61 ggcgccgccc aggacgatga ccaggaacga cttgaaggtg tagacctggc ccatctccgg
      121 ctggatcgcc accatcaccg cgaccagcga gccgcccgcg gcggccagcg ccgagcccag
      181 gccgaacgcg agcaggtgga tgcgctgcac gtcgatgccg cacacctggg cgacctcgcg
      241 gctctgggag actgcccgga tggccttccc caggcgggta gagcggagga agacgaagga
      301 cgccgcggtg atcccgatcg cgaaggcgca ggcggcgagg cgcggcttgg agagcgcgaa
      361 gggccccacc acgtaggacc cggtcaggta ctcgaccgag cgcaggtccg aggtgaacgc
      421 gagctgggcc aggttgacga gcgcgatcga gaccccgaag gtcagcagga gcgaggagag
      481 ctcgggcgcg tccaccaccc ggcgcacaag gaagcgctgc agcgccaccc cgatcaggaa
      541 gagcaccggc atcgacacga ggatcgagag gtacgggttg aggccgaagc gctggaagag
      601 ccagaacgtg gtgtaggcgc ccagcatcat gagcgggccg tgggccacgt tgaggacccg
      661 catgaccccg aagatcagcc cgagaccgag cgccaccatc gcgtagatgg agccggccag
      721 gagaccggag atcaccacct ggccgaccat cgttggcgtg atcaggacgc tcacggcccc
      781 tccgaagcgc gacgacgccc gccggagacc cggcgggcgc ctggtgagct tatcgcttgc
      841 cccactccgg cgtcggatag cgcagcttgc cgctcgcgag gtcgtccggc cacaccacca
      901 cccgcttgcc gtcctgccac tggagcatga ccatcttgtg ggcgacctgg aagccgtccg
      961 gctcgacctt gtagtcgccg aacgcggtct tgatctccat cttcaggagc tgctcgcgga
     1021 cacggtcggc gtcgagggtg cccgcccgct tgaccgcctc cccgtagatc aggcagcccg
     1081 cgtagcccgc cgccgcgtgg taggagggct cgtgcttgaa cttcttggtg taggcggcga
     1141 ggaactcctt ctggccggga tacggcagcg actcgtccca ctgggtggag ccgtagacgt
     1201 actccgcgtt ctgcttgagc agatcgtaga actcgggcag gtcaccgccc accgtgagcc
     1261 cgaacatctt cgggttcacg ttcagctcct tcatctgccg ggtgatggcg accgcgtcgt
     1321 cgaagtaggt gcccgccgcg atgacgtcgg ggttcgcggc cttgatcttc acgagcagcg
     1381 ccgagaagtc ggtgttgccc ttcgggtagg cctcctgcag caccacctgc atgccccgct
     1441 tcttggccgc ctccgcggtg ccggcggcgg aggccttggg gaagagggtg tcctcgttga
     1501 tgatcgcgac cgtcttgagc ccgcgcttgg ccgccatgtc gatcaggccg tcgagatagt
     1561 tctcggccgg ggtgatgacc atgaagatgt acttccggcc cttcttgaag atcgaggtgg
     1621 tggccgcgag cggcgccacc atcaccttct tgtacttctc ggtgacgttg gccaccgcct
     1681 cgctcaccgc cgaggagtag ggccccatca ccgcgtccac cttgtcctcg gtgatgagct
     1741 tctcgtagag cttcaccgcg gtcgccggct gcgattggtc gtcgtagatc actagctcga
     1801 ccttccggcc gagcagcccg cccttgccgt tcagctcgtc gatgcagatg tcgtagcctt
     1861 ccttctgata gctgccgggc ttggcgtagg tgccggtgac cgacatcgag gcgccgatct
     1921 tgatgggagt ctgcgcgccg gccggcagcg gcagcgacgc cgccaccacg cccgcgatag
     1981 ccaccggggc caggcgtttg cccagacatc tgcccagata tctaaaggac gtcatgatca
     2041 tgagaatcct cctgagaggc gtgatcgagc ggatgggccc gtgctggaat gggcctttct
     2101 tttagcaaac atggctcagg gtgtcaaggt tccggggcca cgagacggcc ccggatccct
     2161 tgacgccggg cgggtccgcg tcaatgatgc gctgtcatgg cccgagagat cgtccgcgcc
     2221 gtccaccacc ccgacaagcg acacgtggag atgccctacg ctccggccct gctcgtgcgc
     2281 gggggtcagc tcctgtttct ctccgggctc accgccgccc cggtctatca cagccatccg
     2341 caccgcgagg aggagttcga cctgcccccg cgcatgcgtg agcaggcggt gctcctgatg
     2401 gagaacctca agaccacgct ggcggcggcg ggctgcacgc tggccgacgt ggtctcggcc
     2461 acccgctacc tcaccgacgt cgccgagcag gacgatctca acgcggtgtg ggcctcctac
     2521 ctgggcgccc atcgccccac cacgaccacg gtggaggtct cacgcctcgc cacccacgcc
     2581 aactgcaaga tcgagatcag cgtcatcgcc tgctttgcga ggcgcagcca aaggcgagcc
     2641 gagcgcaaac aagaagagct tacaagaagt agccgtaaga ccacccgccc ggggcgcaag
     2701 accgcccgcc ggaaatgacc gccaccctcg gcttcgtcgg c
//
LOCUS       DATMCV010000763         4264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_38553, whole genome shotgun sequence.
ACCESSION   DATMCV010000763 DATMCV010000000
VERSION     DATMCV010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38553"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VNU03_17505"
     CDS             <1..1049
                     /locus_tag="VNU03_17505"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HWP76323.1"
                     /translation="GLIFVGPSWRVIQQMGDKIAARRLMAAAGVPVVPGSDGPVDSVD
                     AAREVAARAGYPVMLKAAAGGGGIGMVKVADEAGLPAAFASAQRRAQAAFGSGGLFLE
                     RYVERARHVEVQVFGDASGSVVHLHERECSIQRRHQKLVEESPAPRLSAALKARLTEA
                     AVTGARTVGYVNAGTMEFIVVGDEFYFLEMNTRLQVEHPVTEEVTGLDLVQAQLRVAA
                     GEPLPWSQAQIPQRGASIECRIYAEDPAKGFMPSPGRLTRLVLPEGPGVRLECGVAEG
                     VEVSVHYDPLLAKLITTGRDRDEAIARMAAALDGFVVEGVKTAIPFHQRVMRSDLFRS
                     GSVHTQLIEQGAFS"
     gene            1071..2696
                     /locus_tag="VNU03_17510"
     CDS             1071..2696
                     /locus_tag="VNU03_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP76324.1"
                     /translation="METTLTEARIRATTGAGHWRDESLEGYLDRWARERPSRTALVDA
                     RGRYSWEDLARAVDRVAHGLRAHGLEPGGVVSCQLPNWNEVVVLFLAALRLGAVVNPI
                     PPTYRASELRFMLGLLESQVAVVPARFRGFDHAGMLDALRPALPRLRSVFVARGDGPG
                     GTTPLAALTEEPWEARSDRRPLGGTDPNTVHEVVFTSGTTGEPKGVMHTQNTTLSTLH
                     RAIERLELSDRDVMLMASTVGHQTGFLFGYCLNLLLGATTVWMDIWNVEEAARLIEAE
                     GVTLTMGATPFLRDLTYAEARQDLGSLRLFIAAGAPIPRALVRDARARLGCVVSAGWG
                     MTEDGLVTCNGLRDPDEKICGSDGFPLPGMELRIVDDDGAPAPIGTEGDLLARGAAQF
                     VGYFRRPQFTAEAHTADGWFRTGDRATIDRDGYLAITGRAKDIIIRGGENIPVVEVEN
                     LLYTHPKIAGVAIVAMPDPRLGERACAVVIPREGQTLTLAEIVGFLERHELARQKLPE
                     RLELVSEFPTTPSGKIQKYKLRDLIVQRMERPA"
     gene            2693..3490
                     /locus_tag="VNU03_17515"
     CDS             2693..3490
                     /locus_tag="VNU03_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP76325.1"
                     /translation="MNLNLEGKSVVVTGGGSNIGRAISLAFAREGAHLTVAEIDEGQG
                     GKVVAEARGLGAASATLVPTDVTRWESVQAMVREVEGRAGRVDVLVNNVGWTYDRLFV
                     EKERTEWEREIQLNLWGMINCTRAVLDGMIARKGGVIVSMGSDAGRMGEFREAVYGAC
                     KAGVIALSKSLAREVGRHGIRLNVVCPGMTIPATEEDFGGLSMWAADANRAFSTPEMQ
                     ARIAKAYPLRRVGKPDDVAPTVVFLASDAAAFITGQTVSVSGGYTMM"
     gene            3508..>4264
                     /locus_tag="VNU03_17520"
     CDS             3508..>4264
                     /locus_tag="VNU03_17520"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP76326.1"
                     /translation="MDFRAITCATGDGIARITLNRPAQLNAISPELLEDLDRACALVE
                     GDAGVRAVTLTAAGRTFCAGADLRAVRELSPDPVRWSAFMGLWHRVFDRIEALPVPVI
                     AGVHGLALAGGLELVLVADLVVADEAARLGDQHANFGLVAGGGGSQRLPRLIGARRAK
                     ELMLLGGWLSAGDALAWGLVNRVVPAGTVGAAVDEMAAALVAGSVSANRTVKALVNRA
                     LDCDLADGLELERRLVAEHMRSADAAEGLRAFAE"
BASE COUNT          573 a         1481 c         1627 g          583 t
ORIGIN      
        1 cgggcctgat cttcgtgggg ccgtcctggc gcgtgatcca gcagatgggc gacaagatcg
       61 cggcgcgccg gctcatggcc gcggccgggg tgccggtggt gccgggcagc gacggcccgg
      121 tggactccgt cgacgcggcg cgagaggtcg cggctcgcgc gggctacccg gtgatgctca
      181 aggcggccgc gggcgggggc ggcatcggca tggtgaaggt cgcggacgag gcggggctgc
      241 ccgccgcgtt cgcctcggcc cagcgacggg cgcaggccgc gttcggctcg gggggcctct
      301 tcctggagcg ctacgtggag cgcgcccgtc acgtcgaggt ccaggttttc ggcgacgcga
      361 gcgggagcgt cgtccacctg cacgagcgcg agtgctcgat ccagcgccgg caccagaagc
      421 tggtcgagga gagccccgcc ccgcgcctgt cggccgcctt gaaggcccgc ctgaccgaag
      481 ccgcggtgac cggcgcccgg acggtgggct acgtcaacgc gggcacgatg gagttcatcg
      541 tggtgggcga cgagttctac ttcctcgaga tgaacacccg gttgcaggtc gagcacccgg
      601 tgaccgagga ggtgaccggg ctcgacctgg tgcaggcgca gctgcgcgtg gccgcgggcg
      661 agccgctgcc gtggagccag gcccagatcc cccagcgcgg ggccagcatc gagtgccgga
      721 tctacgccga ggatcccgcc aagggcttca tgccgtctcc cggccggctg acccggctgg
      781 tgctgcccga gggcccgggc gtccggctcg agtgtggcgt ggcggagggc gtggaggtct
      841 cggtgcacta cgatcccctc ctcgccaagc tgatcaccac cggccgggac cgcgacgagg
      901 cgatcgcgcg gatggcggcg gccctcgacg gcttcgtggt cgagggagtc aagaccgcga
      961 tcccgtttca ccagcgcgtc atgcggagcg acctcttccg ctccgggtcg gtgcacaccc
     1021 agctgatcga gcagggcgcc ttcagctgag gcgccgtctc cggggccggc atggagacca
     1081 ctctcaccga ggcgcgcatc cgggccacga ccggggcggg tcactggcgc gacgagagcc
     1141 tcgagggcta cctcgaccgg tgggcgcgcg agcgcccctc ccggaccgcg ctcgtcgacg
     1201 cgcgcggtcg gtactcgtgg gaggacctgg cccgggcggt cgaccgggtc gcccacggcc
     1261 tgcgggcgca cgggctcgag ccgggagggg tcgtctcctg ccagctcccg aactggaacg
     1321 aggtggtcgt gctcttcctg gccgcgctcc gcctgggcgc ggtggtcaac ccgatcccgc
     1381 cgacctaccg ggcgagcgag ctgcggttca tgctcggcct gctggagtcg caggtggcgg
     1441 tggtgccggc ccgcttccgc ggcttcgacc acgcgggcat gctcgacgcg ctccggcccg
     1501 cgctgccccg gctccggtcc gtgttcgtgg cccggggcga cgggcccggc ggcaccacgc
     1561 cgctcgccgc tctcaccgag gagccgtggg aggcgcggag cgatcgccgg cccctcggcg
     1621 gcaccgaccc caacacggtc catgaagtgg tgttcacctc cggcaccacg ggggagccga
     1681 agggcgtgat gcacacccag aacaccacgc tctccacgct ccaccgggcg atcgagcggc
     1741 tcgagctgtc ggaccgggac gtgatgctga tggcgtccac cgtcggccac cagactggct
     1801 tcctcttcgg ctactgcctc aacctgctac tgggcgccac cacggtgtgg atggacatct
     1861 ggaacgtgga ggaggcggcg cggctgatcg aggccgaggg cgtcacgttg acgatggggg
     1921 cgaccccgtt cctgcgcgac ctcacctacg cggaggcccg gcaggacctg ggctccctcc
     1981 ggctgttcat cgcggcgggc gcgcccatcc cgcgcgcgct cgtgcgcgac gcgcgggcgc
     2041 ggctcggctg cgtggtctcg gcggggtggg ggatgaccga ggacgggctc gtcacgtgca
     2101 acgggctccg ggacccggac gagaagatct gcggcagcga cggctttccg ctgccgggca
     2161 tggagctgcg aatcgtcgac gatgacggcg ccccggcgcc catcgggacc gagggcgatc
     2221 tgctggcccg gggagccgcc cagttcgtcg gctacttccg gcggccgcag ttcaccgcgg
     2281 aggcccacac cgccgacggc tggttccgca ccggagaccg cgccaccatc gatcgcgacg
     2341 gctacctcgc catcactggg agagccaagg acatcatcat ccggggcggg gagaacatcc
     2401 cggtcgtgga ggtcgagaac ctgctctaca ctcatccgaa gatcgcgggc gtcgccatcg
     2461 tggccatgcc cgatccccgc ctgggcgagc gggcgtgcgc ggtggtgatc ccccgggagg
     2521 ggcagacgct cacgctcgcg gagatcgtcg ggttcctcga gcggcacgag ctggcgcgcc
     2581 agaagctgcc ggagcgcctc gagctggtct ccgagttccc cacgaccccg agcggcaaga
     2641 tccagaagta caagcttcgc gacctgatcg tccagcgcat ggagaggccg gcatgaatct
     2701 caatctcgag ggcaagagcg tggtggtcac cgggggcggc tcgaacatcg gacgcgccat
     2761 cagcctggcc ttcgcgcggg agggcgcgca cctgaccgtc gccgagatcg acgagggcca
     2821 gggcggcaag gtggtggcgg aggcccgggg cctgggggcc gcgtccgcga cgctggtgcc
     2881 caccgacgtg acccgctggg agtcggtcca ggccatggtc cgcgaggtcg agggccgcgc
     2941 gggccgcgtg gacgtgctcg tgaacaacgt gggctggacg tacgaccggc tcttcgtcga
     3001 gaaggagcgc acggagtggg agcgcgagat ccagctcaac ctctggggca tgatcaactg
     3061 cacccgcgcg gtgctcgacg gcatgatcgc ccgcaagggc ggcgtgatcg tgagcatggg
     3121 ctcggacgcc gggcgcatgg gcgagttccg cgaggcggtg tacggagcct gcaaggccgg
     3181 ggtgatcgcc ctcagcaaga gcctggcccg cgaggtcggc cgtcacggca tccgtctcaa
     3241 cgtggtgtgc ccgggcatga ccatcccggc gaccgaggag gacttcggcg gcctgagcat
     3301 gtgggccgcc gacgccaacc gggccttcag caccccggag atgcaggcgc gcatcgccaa
     3361 ggcctacccg ctgcggcggg tcggcaagcc ggacgacgtg gcccccaccg tggtgttcct
     3421 cgcctccgac gcggccgcct tcatcaccgg gcagaccgtg tcggtgagcg gcggctacac
     3481 gatgatgtga ggcgggagag accccccatg gactttcgag cgatcacctg cgcgaccggc
     3541 gacggcatcg cgcgcatcac gctgaatcgg cccgcccagc tcaacgcgat cagccccgag
     3601 ctgctcgagg atctcgaccg ggcctgcgcc ctcgtggagg gtgacgccgg cgtccgcgcg
     3661 gtcaccctca ccgcggcggg gcggacgttc tgcgccgggg cggacctgcg cgcggtgcgg
     3721 gagctctcgc ccgatccggt gcgctggagc gccttcatgg gtctctggca tcgcgtcttc
     3781 gatcggatcg aggcgctgcc ggtcccggtc atcgcggggg tgcacgggct cgcgctcgcc
     3841 ggtggcctcg agctggtcct ggtggccgat ctagtcgtcg ccgacgaggc ggcgaggctc
     3901 ggcgaccagc acgccaactt cggcctggtc gcgggcggcg ggggcagcca gcgcctgccg
     3961 cgcctgatcg gggcccggcg cgcgaaggag ctgatgctcc tcggcggctg gctgagcgcg
     4021 ggcgacgcgc tggcgtgggg cctcgtcaac cgggtcgtgc ccgcggggac ggtgggtgcc
     4081 gcggtggacg agatggcggc cgccctcgtg gcgggaagcg tctccgccaa ccggaccgtg
     4141 aaggcgctgg tcaaccgcgc gctcgactgc gatctcgccg acgggctcga gctggagcgc
     4201 cggctggtgg ccgagcacat gcgctcggcc gacgccgcgg aggggctgcg cgccttcgcc
     4261 gaga
//
LOCUS       DATMCV010000764         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_773679, whole genome shotgun sequence.
ACCESSION   DATMCV010000764 DATMCV010000000
VERSION     DATMCV010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773679"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VNU03_17525"
     CDS             complement(<1..826)
                     /locus_tag="VNU03_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HWP76327.1"
                     /translation="MLSPACAEATGALLSALLGVLDRAEWVQRQLFPPQAGRLAQQLA
                     PHAERLAEPLAAFETQAWPDDLRFLRERLAEVARQALELIEAFGAAAGTGEPIGLYRA
                     LRRLGPLQEALYPLYPVLGPVSRWFIEAGRRDDDDLVERLRAGALREDGVRSGILHAQ
                     NDRASRGGLSLYVPETSDGQTPAPLIVALHGGSGHGRDFLWAWLREARTRGALLLSPT
                     ARERTWSIMGGEDVDAPRLVAAVEEVARRHPVDRSRVLLTGMSDGATYALLAGLAED"
     gene            complement(830..1648)
                     /locus_tag="VNU03_17530"
     CDS             complement(830..1648)
                     /locus_tag="VNU03_17530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP76328.1"
                     /translation="MNGKLDRPGGALHFEIIDATPPWLGEAPLIVFHHGIGATADIWA
                     EWLPALVDRFRIARFDTLGFGRSAQPGPGYAWSLDGLVEDVLAVARAAGGGPFHFVGE
                     SLGGTVGLRLASRHPEALLSLTASNASHRGGSIQRAREWREFIGAKGMAAWGEMMTPL
                     RLDRARVSEAQWRWFERAQAEASPDSVLDLADLLIGADLTSELGRIGVPTLLLAPARS
                     PFVPLAVMEAIHAAIPGSELAVFADARHGLPCSHGPACGRALRAFLDRRVPSAA"
     gene            complement(1697..2002)
                     /locus_tag="VNU03_17535"
     CDS             complement(1697..2002)
                     /locus_tag="VNU03_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76329.1"
                     /translation="MLRLLVIALGLGAVVAVPAAAQYRYTNDKGVTKEAQYKLDVPEP
                     YRDAAVWVGPTGVGKPALSEDQRKMKERWDPIQKKADDARKADDARAAERQRLAPTR"
BASE COUNT          304 a          850 c          803 g          285 t
ORIGIN      
        1 tgtcctcggc gaggccggcc agcagcgcgt aggtggcgcc gtccgacatg ccggtgagca
       61 gcacccgcga ccggtcgacc ggatggcgcc gcgccacctc ctccaccgcg gcgacgagtc
      121 gcggagcgtc gacgtcctcg ccgcccatga tcgaccaggt gcgctcccgg gcggtgggcg
      181 agagcaggag cgcgccgcgc gtgcgcgcct cgcggagcca ggcccacagg aagtcgcgcc
      241 cgtggccgct gccgccgtgg agcgcgacga tcagcggcgc cggggtctgg ccatctgacg
      301 tctcgggcac gtagagcgag agcccgccac ggctggcccg gtcgttctgc gcgtgcagga
      361 tgccgctccg cacgccgtcc tcccggagcg cgcccgcgcg caggcgctcc accaggtcgt
      421 cgtcgtcgcg gcggccggcc tcgatgaacc accggctgac cgggcccagc accgggtaca
      481 gcgggtagag cgcctcctgc agcgggccga ggcgccgcag cgcgcggtaa agcccgatcg
      541 gctcgccggt cccggccgcg gcgccgaaag cctcgatcag ctcgagggcc tggcgcgcca
      601 cctcggccag ccgctcgcgc aggaatcgta gatcgtccgg ccacgcctga gtctcgaagg
      661 cggcgagcgg ctccgccagg cgctcggcgt ggggcgcgag ctgctgggcg agccgtccgg
      721 cctggggcgg aaagagctgg cgctgcaccc actccgcgcg atcgagcacg ccgagcagcg
      781 ccgagagcag ggccccggtc gcctcggcac acgcgggcga gagcatgcgc taggcggcgg
      841 agggcacgcg gcggtcgagg aacgcccgga gcgcgcgccc gcaggccggg ccgtgcgagc
      901 agggcagccc gtgccgcgcg tccgcgaaca cggcgagctc ggagccgggg atcgcggcgt
      961 ggatcgcctc catcaccgcg agcggcacga aggggctccg cgcgggcgcg agcaggagcg
     1021 tcggcacgcc gattcgcccc agctccgacg tgagatccgc gccgatgagc aggtcggcga
     1081 ggtcgaggac cgaatcgggc gacgcctcgg cctgcgcgcg ctcgaaccag cgccactgcg
     1141 cctccgacac ccgcgcgcga tccagccgga gcggggtcat catctcgccc caggcggcca
     1201 tgcccttcgc cccgatgaac tcgcgccact cccgggcgcg ctggatggac ccgccccggt
     1261 gggaagcgtt ggacgcggtc aggctcagga gcgcctccgg atgccggctc gcgagccgga
     1321 gcccgacggt accgccgagc gactcaccca cgaagtggaa gggcccgccg ccggccgcac
     1381 gcgccaccgc gagcacgtcc tcgacgagcc cgtcgagcga ccacgcgtac ccgggaccgg
     1441 gctgagcgga gcgcccgaag ccgagggtgt cgaagcgtgc gatccggaag cggtccacga
     1501 gggcggggag ccactcggcc cagatgtcgg cggtcgcgcc gatgccgtga tggaagacga
     1561 tgagcggcgc ttctccgagc cacggaggcg tggcgtcgat gatctcgaaa tgcagcgcgc
     1621 cgcccggacg atcgagcttg ccgttcacct cgcggacggt atcagagcgg tgcggatccg
     1681 gcaagcagcc gggcgactag cgggtcggcg cgagccgctg gcgctcggcc gcccgcgcgt
     1741 cgtccgcctt cctcgcgtcg tccgccttct tctgtatcgg atcccagcgc tccttcatct
     1801 ttcgctggtc ctcgctcaag gccggcttgc ctaccccggt cggcccgacc cagaccgccg
     1861 catcgcggta cggctcgggc acgtccagct tgtactgcgc ctccttggtc acgcccttgt
     1921 cgttcgtgta ccggtactgc gcggcagccg gcaccgcgac gaccgctccc agccccagcg
     1981 caatcacgag caatctcagc atcgttggcc ccccttggcc gctccagacc ttcgcctcga
     2041 ttgtcctgcc gggcgaatta agcccggtcc aatttcggcg gggcgcccgc tcggcgcgat
     2101 cctgccgttt tccttgagcg aacacccccc tccccggggc gcggcgaggc ccgcggccgg
     2161 ctgagctgtc gtgagaccgc ctgcccagac ttacgcggta gttccttcca ctccctgctt
     2221 accaggctcg cgtcggtgct tc
//
LOCUS       DATMCV010000765         3576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_837282, whole genome shotgun sequence.
ACCESSION   DATMCV010000765 DATMCV010000000
VERSION     DATMCV010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837282"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VNU03_17540"
     CDS             complement(<1..286)
                     /locus_tag="VNU03_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP76330.1"
                     /translation="MALRGVSFAVPAGAIVTILGANGAGKTTILKTVSGVMDPQKGSI
                     TLEGRQIQGLDPDRVARLGLSHVPEGREVFPFLTVRENLRMGAYTRRDADG"
     gene            complement(347..1105)
                     /locus_tag="VNU03_17545"
     CDS             complement(347..1105)
                     /locus_tag="VNU03_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76331.1"
                     /translation="MTFFAVEDLGVSFGGIRAVDGVSFEVEAGQVFTIIGPNGAGKTT
                     IFNLISRIYDPTDGRLVFRDEDITRVPPHEIARRGIARTFQNIELFEHASVLQNLLLG
                     RHAHARPRLLQEMLFLPAVRARERAHREAAEKVIDFLELQAYRDSLVVNLPYGVRKVV
                     EMGRALCTEPALLLLDEPSSGLSVEETEDMAFWIQDIRSLLGISVLMVEHDMSLVGAV
                     SDRVLALNYGRVVACGTSREVQEHPDVVKAYLGG"
     gene            complement(1102..2244)
                     /locus_tag="VNU03_17550"
     CDS             complement(1102..2244)
                     /locus_tag="VNU03_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76332.1"
                     /translation="MRFAFKTAYDHDLGLFRDPVQRRWYAGLLAAVLLLPLAVPDYVS
                     DISMVFIYGLCGLSLMVLAGYTGLVSLGHAAFLGIGAYAHVYFVQDLGLPWVVAVALA
                     AALSGASGVLVGLPALRMTGVYLTIATLAFALIIQEAFSRWEHVTHGLKGRPVDKPVI
                     FGVSFASDAAFYFLCLAVLIAGLWLTANILRAPTGRAWVAIRDSEIAAQSMGVHLARY
                     KTMAFAYSAALMGAAGALFAHKIGFLAPDIFSVLLSIQFLLMVVVGGLGSLHGALYGA
                     VFVAMLPVLISEVRDHAPQWAGHLSGLLGKDAGAAVYLTVERFVKQPGLEPGIFGLIL
                     VLFILFEPLGIYGRWRKIQLLFSTFPLYKRATFRRQKTYMRSERLR"
     gene            complement(2254..3126)
                     /locus_tag="VNU03_17555"
     CDS             complement(2254..3126)
                     /locus_tag="VNU03_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76333.1"
                     /translation="MDTILQLALNGLAVGCIYGLVALGFVLIYKATELVNFAQGDLLM
                     LGAFVCYMFVVWYGIGYWTAFALAIGIVGAFGAVLDATVLRRVIGQPQFAVVMLTIGL
                     GAMFRSFASLTWGSEIYTLPTPFSAKTTRLGGVTVSHEYVAIIVGTVVLCALLYAFFT
                     YSRVGVAMQAASQNQLAAYYMGIPVKRMFSLIWAISAGVAAIAGVLLAPVSLIDINLG
                     FIGLKAFAAAVLGGFGSIPGALVGGVTIGLIELFSGAYLPLGVKDVAAYIVLLLVLAI
                     RPQGMFGTVGRKKV"
     gene            complement(3236..>3576)
                     /locus_tag="VNU03_17560"
     CDS             complement(3236..>3576)
                     /locus_tag="VNU03_17560"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWP76334.1"
                     /translation="PRRRGRSSTPRSARPRPSAREKRPTALLHPDDCAALGAPAGTPV
                     RLGNERGEVVAHAEPRPGQQRGVVVVEGVWPNRDFVGGVGINALTSADPGYPNGGAVF
                     HDTAVWIRKL"
BASE COUNT          609 a         1212 c         1293 g          462 t
ORIGIN      
        1 ccccgtccgc gtcccggcgc gtgtaggcgc ccatccgcag gttctcgcgc acggtcagga
       61 aggggaagac ctcgcgtccc tccggcacgt gggagagccc gaggcgcgcg acccggtccg
      121 ggtcgaggcc ctggatctgg cgaccctcga gcgtgatgct gcccttctgc ggatccatga
      181 ctccggacac cgtcttgagg atggtggtct tgcccgcccc gttggcgccc aggatcgtga
      241 cgatggctcc cgcgggcacc gcgaagctga ctccccgcag ggccatgatg gggccgtagt
      301 aggtctccac gttgcgcacg gtgaggagcg cctcggccac cgttcgtcag cctccgaggt
      361 aggccttgac cacgtcggga tgctcctgga cctcccgcga cgtcccgcac gccaccacgc
      421 gcccgtagtt cagcgcgagc acgcggtccg agaccgcgcc caccaggctc atgtcgtgct
      481 cgaccatcag cacgctgatg ccgagcaggc tccgaatgtc ctggatccag aacgccatgt
      541 cctcggtctc ctcgacgctg agcccggagg acggctcgtc gagcaggagc agggccggct
      601 cggtgcagag ggcccggccc atctccacca ccttgcgcac cccgtagggc aggttcacga
      661 ccaggctgtc gcggtaggcc tgcagctcga ggaagtcgat caccttctcg gccgcctcgc
      721 gatgggcgcg ctcgcgggcg cgcaccgcgg ggaggaacag catctcctgc aggagccgcg
      781 gccgggcgtg ggcgtggcgg cccagcagca ggttctggag caccgaggcg tgctcgaaca
      841 gctcgatgtt ctggaaggtg cgcgcgatgc cccgccgggc gatctcgtgc gggggcaccc
      901 gggtgatgtc ctcgtcgcgg aagacgaggc ggccgtcggt ggggtcgtag atgcgcgaga
      961 tcaggttgaa tatggtggtc ttgcccgcgc cgttcggccc gatgatcgtg aacacctggc
     1021 ccgcctcgac ctcgaacgac accccgtcta cggcccggat gccgccgaag ctgaccccga
     1081 ggtcctcgac ggcgaagaag gtcaccggag gcgctccgag cgcatgtagg tcttctggcg
     1141 ccggaaggtg gcgcgcttgt agagcgggaa ggtcgagaag aggagctgga tcttgcgcca
     1201 tcggccgtag atgccgagcg gctcgaagag gatgaagagc accaggatga ggccgaagat
     1261 gccgggctcg aggccgggct gcttcacgaa gcgctccacc gtcaggtaga ccgccgcgcc
     1321 cgcgtccttg ccgagcaggc ccgagagatg gccggcccac tggggcgcgt ggtcgcgcac
     1381 ctccgagatc agcaccggca gcatcgccac gaagaccgcg ccgtagagcg cgccgtggag
     1441 gctgccgagg ccccccacca cgaccatcag cagaaactgg atggagagca ggacgctgaa
     1501 gatgtcgggg gcgaggaagc cgatcttgtg ggcgaagagc gcgcccgccg cgcccatcag
     1561 cgcggccgag taggcgaagg ccatggtctt gtagcgcgcg aggtgcacgc ccatggactg
     1621 cgccgcgatc tcgctgtcgc ggatggccac ccacgcacgg ccggtcggcg cgcgcaggat
     1681 gttggcggtg agccacaggc cggcgatcag gaccgccagg cagaggaagt agaaggcggc
     1741 gtcggaggcg aaggagacgc cgaagatcac cggcttgtcg accggccggc ccttcaggcc
     1801 gtgggtcacg tgctcccacc ggctgaacgc ctcctggatg atgagcgcga aggcgagtgt
     1861 cgcgatcgtc aggtagacgc cggtcatgcg cagcgcgggc aggcccacca gcacgccgga
     1921 cgccccggac agcgccgccg cgagcgcgac cgccaccacc cacggcagcc ccaggtcctg
     1981 cacgaagtag acgtgggcgt aggccccgat gccgaggaag gcggcgtggc ccaggctcac
     2041 caggccggtg tagccggcca gcaccatcag cgagagcccg cagagcccgt agatgaagac
     2101 catgctgatg tcgctcacgt agtcgggcac ggcgagcggc aggagcagca ccgcggccag
     2161 caggcccgcg taccagcgcc gctggacggg atcgcggaag agccccaggt cgtgatcgta
     2221 ggcggtcttg aaggcgaagc gcatggggcg cggtcagacc ttcttccggc ccacggtgcc
     2281 gaacatgccc tgggggcgga tggcgagcac cagcagcagc acgatgtagg ccgccacgtc
     2341 cttcaccccg agcggcaggt aggcgcccga gaacagctcg atgagcccga tggtcacgcc
     2401 gcccaccagc gcgcccggga tcgacccgaa gccgcccagc accgcggccg cgaacgcctt
     2461 gagcccgatg aagcccaggt tgatgtcgat cagcgagacc ggggccagca ggacgcccgc
     2521 gatggcggcc accccggccg agatggccca gatgagcgag aacatgcgct tcaccgggat
     2581 gcccatgtag tacgccgcga gctggttctg ggaggcggcc tgcatggcca cgcccacccg
     2641 cgagtaggtg aagaaggcgt agagcagggc gcagagcacc accgtgccca cgatgatcgc
     2701 cacgtactcg tggctcaccg tcaccccgcc cagccgggtg gtcttggcgc tgaatggcgt
     2761 cggcagggtg tagatttcgg agccccaggt cagggaggcg aacgagcgga acatcgcgcc
     2821 caggccgatg gtgagcatga ccaccgcgaa ctggggctgg ccgatgacgc gccggagcac
     2881 ggtggcgtcg agcaccgccc cgaaggcacc gacgatgccg atggcgagcg cgaaggcggt
     2941 ccagtagccg atgccgtacc agaccacgaa catgtagcag acgaacgcgc ccagcatgag
     3001 caggtcgccc tgggcgaagt tgaccagctc ggtggccttg tagatgagga cgaagccgag
     3061 ggcgaccagg ccgtagatac agcccacggc gaggccattc agggcaagct gcaggatcgt
     3121 gtccagcgca gaccccccgc cgggctccac cggcctgctc cgtccgccgc ggcgaccctg
     3181 tcgcggcgtg acggtaatgg gggcggttat atccggcggg cgccggtcaa gtcaatcaga
     3241 gcttgcggat ccagaccgcg gtgtcgtgga agacggcgcc cccgttgggg tagccggggt
     3301 cggcgctggt gagcgcgttg atgccgacgc cccccacgaa gtcgcgattc ggccagaccc
     3361 cttccacgac caccacgccc cgctgctggc ccggccgggg ctcggcgtgc gcgaccacct
     3421 cgccccgctc gttgccgagc cggacggggg tgccggcggg agcgcccagc gccgcacagt
     3481 cgtcgggatg gagcagggcg gtgggccgct tctcgcgcgc gctaggacgg ggtctcgctg
     3541 aacgtggagt tgaggaacgt ccgcgccggc gcggcc
//
LOCUS       DATMCV010000766         4009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_910507, whole genome shotgun sequence.
ACCESSION   DATMCV010000766 DATMCV010000000
VERSION     DATMCV010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910507"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1234
                     /locus_tag="VNU03_17565"
     CDS             308..1234
                     /locus_tag="VNU03_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP76335.1"
                     /translation="MKTVTARTTTQPARVRYKNARVAGLDIFYREAGPKDAPTLLLLH
                     GFPTSSQMFRNLIPALADQYHVVAPDYPGYGHSSMPSRDTFAYTFDNLAQVIDQFTEQ
                     LGLETYALYVQDYGAPIGYRLAVKHPERVTAIVVQNGNAYEEGLDNDFWKPLKEYWKD
                     PASKDKRDAIRGMLTYEATRWQYMTGARNPDLVSPDGAAEDQFLLDRKGNDEIQLDLF
                     LSYGSNPPLYPAWQDYFRKHQPPMLIAWGKNDQIFPPAGAEPYKRDLMTLEYHLIDAG
                     HFALETDGETIAALMREFLGKHVTKKSERTPA"
     gene            1269..1538
                     /locus_tag="VNU03_17570"
     CDS             1269..1538
                     /locus_tag="VNU03_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008384793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HWP76336.1"
                     /translation="MSRPDGGALRVSRRAALWTGATGLAAALVPARAGAQTKIAPKLV
                     QYQEKPKGPQECDNCLQFVAPAACKLVDGKINPKGWCQLYAAKPK"
     gene            1637..3094
                     /locus_tag="VNU03_17575"
     CDS             1637..3094
                     /locus_tag="VNU03_17575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HWP76337.1"
                     /translation="MLVTGTHDPYLVALSILVASFASYTALDLAAHAGAARGLARRLW
                     LVAAAITMGGGIWSMHFVAMLAFIMPVPLAYDLGVTALSLLVAIVVTSASFYVISRPT
                     VSPLRVALSGIFMGFGIVAMHYTGMAAMRGHADLSYERPFVALSLVIAIGASTAALWL
                     AFRTTDLGQRLVAAVVMGLAISGMHYTAMRAAIFTVHGPLNEASKYAGLDQTTLALVV
                     AGTTFVLLAFALGASLFEQQCAEEALRQARADLAHVNRVTTMGALTASLAHEVNQPIT
                     AVVTNAYACLHWLEGETPNLAEARASAMSIVEDGTRAADIIGRTRLLFTKSAPRREPV
                     DLNEVIREMVVLLRSETARHSISVRTDLAPHLPRVMGDRVQLQQVMMNLMINSVDAMR
                     DVEGARELFIRSQGAGDEQLTVCVSDTGVGLPPQQAEQIFYAFFTTKPQGTGMGLSIS
                     HSIVESHHGRLWAVDNSPRGACFHVALPAAVDPYD"
     gene            complement(3114..>4009)
                     /locus_tag="VNU03_17580"
     CDS             complement(3114..>4009)
                     /locus_tag="VNU03_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HWP76338.1"
                     /translation="FFRTALAPPAAGAPARGTAAPPTSAWSSRQLYLAHFALTDTAGR
                     RFHAWSRLEREALGLAGAQASPFRVWIEDWSAESEVPGGLPVRLRAAEGDVAIDLVLS
                     SQKPVTLQGDHGLSQKGPEPGNASYYYSLSRMSVRGTVRVGGEPLPVSGLAWMDREWS
                     TSALGPDLVGWDWVALQLDDGRDVMLYRLRRRDGSADPHSAGTLIAADGATRPLAPGD
                     VTLEALDHWTSPRSRVRYPSRWRLAIPGAGLSLEITPRLPDQELIVGTRYWEGAVGVA
                     GTAAGRPIAGRGYVELVGYGE"
BASE COUNT          665 a         1426 c         1324 g          594 t
ORIGIN      
        1 agccgcgtgg tgctggaaca ggcgaccgtc gagagcatca gaagcccggc cagcgccccc
       61 atccgaagta cggttactga actcataagt ctgtcccctt cccctgaggt ggtggttggt
      121 cgatccgcgc ttgtgatccc acgcgtggcg cgacgggtta cgaccgcccg gctttccgca
      181 agaaccggca agcgggcctg tcagattcct gccgaaagcc ttccgacgcg tcaccggtat
      241 cgtcgccgca ccctcccaca cgtacgggta gccgtcggta tcccacccac gcgaggagaa
      301 ggacgccatg aagaccgtca ccgcgagaac gaccacccaa cccgcgagag tccggtacaa
      361 gaacgcccga gtcgccggcc tggacatctt ctaccgcgag gccggtccga aggacgcgcc
      421 gacgctcctg ctcctgcacg gcttcccgac cagctcgcag atgttccgca atctcatccc
      481 ggcgctggcg gaccagtatc acgtcgtcgc gccggactac cccggctacg gccacagctc
      541 gatgccgtca cgggatacct tcgcgtacac cttcgacaac ctggcgcagg tgatcgacca
      601 gttcaccgag cagctcgggc tcgagacgta cgccctgtac gtgcaggact acggggcccc
      661 gatcggttac cgtcttgccg tcaagcaccc ggagcgggtc accgccatcg tcgtccagaa
      721 cggcaacgcc tacgaggagg gcctcgacaa cgacttctgg aagccgctca aggagtactg
      781 gaaggacccc gccagcaagg acaagcgcga cgccattcgc gggatgctca cctacgaggc
      841 gaccagatgg cagtacatga ccggcgccag gaacccggac ctcgtgagcc cggacggggc
      901 ggccgaggac cagttcttgc tcgaccgcaa ggggaacgac gagatccagc tcgatctctt
      961 tctcagctac ggcagcaatc cccctctcta tcccgcgtgg caagactact tccggaagca
     1021 tcaaccgccg atgctgatcg cgtggggaaa gaacgaccag atcttccccc ccgccggagc
     1081 ggagccctac aagcgcgatc tgatgacgct ggaatatcac ctgatcgacg ccggtcactt
     1141 cgccctggaa acggacggcg agaccatcgc ggcgctgatg cgagagttcc tcggcaagca
     1201 cgtgaccaaa aaatccgaaa ggacaccggc atgaccgact accacggccc cgttctcgag
     1261 aagacgcgat gagccggccg gacggaggag cgctcagggt ctcgcgacgc gcggcgctgt
     1321 ggacgggcgc gaccggtctg gccgccgcgc tcgtcccggc ccgggccggc gcacagacca
     1381 agatcgcgcc gaagctggtg caatatcagg agaagccgaa ggggccgcaa gaatgcgaca
     1441 actgcttgca attcgtggcc ccggccgcct gcaagctggt ggacggtaag atcaacccga
     1501 agggctggtg ccagctctac gccgcgaagc cgaagtagcc ggctcgcggg cgcgggccgg
     1561 ccttccggtg tagacttgcg gccgagtccg cggacggtgc gcccactgtc tctggtccga
     1621 caattggggc aacccgatgc ttgttaccgg aacacacgat ccgtacctcg tcgctctatc
     1681 catcctggtc gcctccttcg cgtcctacac cgccctcgat ctggccgcgc acgcgggagc
     1741 cgcccggggg ctggcgcgtc ggctgtggct ggtcgccgcc gcgatcacga tgggaggcgg
     1801 catatggtcg atgcacttcg tcgccatgct cgcgttcatc atgccggtac cgctcgccta
     1861 cgatctcggc gtgaccgccc tctccctcct ggtggcgatc gtcgtgacca gtgccagctt
     1921 ctacgtcatc agccgcccga ccgtctcgcc gcttcgcgtc gccctcagcg gcatcttcat
     1981 gggattcggc atcgtcgcca tgcactacac cggcatggcg gccatgaggg ggcacgccga
     2041 cctcagctac gagcgtccgt tcgtcgcgct ctccctggtc atcgccatcg gcgcgtcgac
     2101 cgcggccctg tggctggcgt tccggaccac cgatctcggc caacgactcg tcgccgccgt
     2161 cgtcatgggc ctggccatct cggggatgca ctacaccgcg atgcgcgcgg ccatcttcac
     2221 ggttcacggc ccgctcaacg aggcctcgaa gtacgcgggg ctcgaccaga ccacgctggc
     2281 gctggtggtc gcgggcacga cgttcgtcct gctcgccttc gcgctgggcg cctccttgtt
     2341 cgagcagcag tgcgcggaag aagcgctgcg ccaggcgcgg gccgatctcg cccacgtcaa
     2401 tcgggtgacg accatgggcg cgctgaccgc gtccctggcc cacgaggtga accagccgat
     2461 caccgcggtc gtcaccaacg cctacgcctg cctgcactgg ctcgaggggg agaccccgaa
     2521 tctcgcggaa gcgcgcgcgt ccgcgatgag catcgtggag gacggaacgc gcgcggcgga
     2581 catcatcggc cggacgcgcc tgctcttcac gaagagcgct ccgcgacggg agccggtcga
     2641 tctgaacgag gtcatccgcg agatggtcgt cctcctgcgc agcgagacgg cccgccactc
     2701 catctcggtc cggaccgacc tagccccaca tcttccccgg gtcatgggag atcgcgtgca
     2761 gctgcagcag gtcatgatga acctcatgat caacagcgtc gacgcgatgc gggacgtgga
     2821 gggtgcgcgc gagctcttca tcagatcgca gggggcgggc gacgagcagc tcacggtgtg
     2881 cgtcagcgac accggcgtgg ggctacctcc gcagcaggcg gagcagatct tctacgcctt
     2941 tttcaccacc aaaccccagg ggaccgggat gggactatcg atcagccact ccatcgtcga
     3001 atcgcaccac ggccgcctgt gggcggtcga caattccccg cgcggcgcgt gctttcacgt
     3061 cgcgctacct gccgcggtcg acccgtacga ctgacgggcg accgcacctt cgatcactcc
     3121 ccgtagccga ccagctccac gtagccgcgg cccgcgatcg ggcggccggc cgcggtgccc
     3181 gcgacgccga ccgcgccctc ccagtagcgc gtgccgacga tcagctcctg atcgggcagg
     3241 cgcggggtga tctcgaggct gagcccggct cccgggatgg cgagacgcca gcggctcggg
     3301 tagcgcacgc ggctccgcgg gctcgtccag tggtcgaggg cctcgagcgt cacatcaccc
     3361 ggggcgagcg gccgcgtggc gccgtcggcg gcgatcagcg tgccggcgct gtgcgggtcg
     3421 gcgctgccgt cgcgccggcg gaggcggtag agcatgacgt cgcgcccgtc gtcgagctgg
     3481 agggcgaccc agtcccagcc gaccaggtcc gggccgagcg cgctggtgct ccactcgcga
     3541 tccatccatg cgaggcccga gaccgggagc ggctcacctc ccacgcgcac ggtgccgcgg
     3601 acgctcatcc gggagagcga gtagtagtag gaggcgttgc cgggctcggg gcccttctgg
     3661 ctgaggccgt ggtcgccctg gagagtcacc ggcttctggc tcgaaaggac caggtcgatg
     3721 gcgacgtctc cctcggcggc gcggagccgt accggcagcc cacccggcac ctcgctctcc
     3781 gccgaccagt cctcgatcca gacgcggaac gggctcgcct gcgccccggc cagcccgagc
     3841 gcttcccgct cgagccggct ccaggcgtga aagcgccgcc ccgcggtgtc ggtgagcgcg
     3901 aagtgcgcga ggtagagctg gcgggacgac catgcagacg tgggcggggc ggcggtacca
     3961 cgcgcggggg cgccggccgc gggcggcgcc agcgcggtgc ggaagaagg
//
LOCUS       DATMCV010000767         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_38885, whole genome shotgun sequence.
ACCESSION   DATMCV010000767 DATMCV010000000
VERSION     DATMCV010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38885"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VNU03_17585"
     CDS             complement(<1..913)
                     /locus_tag="VNU03_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HWP76339.1"
                     /translation="MSPAPRLSARDCLPRDAAEALLIGRAWSPAEDGPVVVAVRGDEV
                     VDLTRQYPTTSGLLNTAMPAQVRAAIKTAAAVGSLEALAANSVEGARDATRPWLLAPA
                     DLHAVKASGVTFVASLLERVIEERAKGNPALADGARREIQAIVGADLRGIKPGSPEAG
                     RVKEVLVAKGMWSQYLEVGIGPDAELFSKCPPMAAVGTGARVGVHPASVWSNPEPELV
                     LVINRDGAIVAVTLGNDVNLRDVEGRSALLLGRAKDNNASCAMGPFFRLLDERFTLDH
                     ARRVTFETVVEGTDGFRIAHQTQVSEIS"
     gene            complement(910..2070)
                     /locus_tag="VNU03_17590"
     CDS             complement(910..2070)
                     /locus_tag="VNU03_17590"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWP76340.1"
                     /translation="MLTIDGHCEPRFTAVREAFFRNFTERGDVGAAVCVYLDGVRVVD
                     CWGGHADAARTRPWTADTIVSVASTTKGMVALCAHMLAERGKLDLDAPVARYWPEFAQ
                     AGKQDIPVRWLLSHRAGLPAIRPTLPAEALLDWSAMTAALAESAPWWTPGSQHGYHAI
                     TFGHLVGEVIRRVDGRSVGAFLRDEVTGPLRADFFIGVPEEIDARAAVVLPPPPASEP
                     TIWDTILADPDSVSGRTFLNPPRTADLVNTRAWRAAEIPAANGHTSARGVARVYAALA
                     RGGELDSVRLLTPATIDRAIVAQSSGRDAVLTLPTRFATGFMLPLPGGVFECGPGAHT
                     FGHPGRGGSIGFADPGARLAFGYVTNQYVTGTARHPDRRVASLVDAVYAALR"
     gene            complement(2087..>2370)
                     /locus_tag="VNU03_17595"
     CDS             complement(2087..>2370)
                     /locus_tag="VNU03_17595"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the hydrolysis of a monocarboxylic acid
                     amid to form a monocarboxylate and ammonia; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP76341.1"
                     /translation="IERAWPVDNSDLLLTFTVNGRSVPYLHGLCYPAVSTVPGQPATA
                     FPVGLSREGLPIGLQAIGPYLEDLTTIRFTALLAREIGGYRKPSGYDES"
BASE COUNT          307 a          851 c          911 g          301 t
ORIGIN      
        1 ggctgatctc gctcacctgg gtctggtgcg cgatgcggaa gccgtcagtg ccctcgacca
       61 ccgtctcgaa ggtcacccgg cgggcgtggt cgagggtgaa gcgctcgtcg aggagccgga
      121 agaacggccc catcgcgcac gaggcgttgt tgtccttggc gcggccgagc agcagcgcgc
      181 tgcgcccctc cacgtcgcgc aggttcacgt cgttgccgag ggtgaccgcc acgatcgccc
      241 cgtcgcggtt gatcaccagc accagctccg gctccgggtt gctccacacc gaggccgggt
      301 gcacgcccac ccgcgcgccg gtgcccaccg cggccatcgg cgggcatttg ctgaagagct
      361 ccgcgtccgg cccgatgccg acctcgaggt actgcgacca catccccttg gccaccagca
      421 cctctttgac ccgtcccgcc tccggcgagc cgggcttgat gccgcgcagg tcggcgccca
      481 cgatcgcctg gatctcgcgg cgcgccccgt cggccagggc cgggttgccc ttggcgcgct
      541 cctcgatcac gcgctcgagc aggctcgcca cgaaggtgac gccgctcgcc ttgaccgcgt
      601 gcaggtcggc gggcgcgagg agccacggcc gcgtggcgtc gcgcgcgccc tccacgctgt
      661 tggccgcgag cgcctcgagc gagcccaccg ccgccgcggt cttgatcgcg gcgcggacct
      721 gggccggcat ggcggtgttg agcaggccgc tcgtggtcgg gtactggcgg gtgaggtcga
      781 ccacctcgtc gccgcgcacc gcgacgacca ccgggccgtc ctcggccggg gaccaggcgc
      841 gcccgatcag cagcgcctcc gccgcgtccc gtggcaggca gtcgcgcgcg gagagccggg
      901 gcgccgggct catcggagcg ccgcgtacac cgcgtcgacc aggctcgcga cgcgccggtc
      961 gggatgccgc gcggtgccgg tgacgtactg gttggtcacg tagccgaagg cgaggcgcgc
     1021 gcccgggtcg gcgaagccga tcgagccgcc gcggcccggg tgaccgaagg tgtgcgcgcc
     1081 aggtccgcac tcgaagaccc cgccgggcag cgggagcatg aagccggtcg cgaagcgggt
     1141 gggcagggtc agcaccgcgt cgcggcccga cgactgggcg acgatggcgc ggtcgatggt
     1201 ggcgggcgtg agcagccgca ccgagtcgag ctcgccgccc cgcgcgagtg cggcgtagac
     1261 gcgcgcgacc ccgcgggcgc tggtgtggcc gttggcggcc gggatctccg ccgcgcgcca
     1321 ggcgcgggtg ttgaccaggt ccgcggtgcg cgggggattg aggaacgtgc ggccggagac
     1381 cgagtcggga tcggccagga tcgtgtccca gatggtgggc tcggacgccg gcgggggcgg
     1441 caggacgacg gcggcgcgcg cgtcgatctc ctcgggcacg ccgatgaaga agtcggcgcg
     1501 cagcgggccg gtcacctcgt cgcgcaggaa cgcgcccacg ctgcgcccgt cgacccggcg
     1561 gatcacctcg cccaccaggt ggccgaaggt gatggcgtgg tagccgtgct gactgcccgg
     1621 cgtccaccac ggcgcgctct ccgcgagggc cgcggtcatg gcggaccagt cgagcagcgc
     1681 ctccgcgggc agggtcgggc ggatggcggg cagccccgcg cgatggctca ggagccagcg
     1741 caccgggatg tcctgcttgc cggcctgggc gaactccggc cagtagcggg ccaccggcgc
     1801 gtccagatcg agcttgccgc gctcggccag catgtgggcg cagagggcga ccatcccctt
     1861 cgtggtggag gcgacgctga cgatggtgtc ggccgtccac ggccgcgtgc gggccgcgtc
     1921 cgcgtggccg ccccagcagt cgaccacccg caccccgtcg aggtagacgc acaccgcggc
     1981 gcccacgtcc ccgcgctcgg tgaagttgcg gaagaacgcc tcgcggaccg cggtgaagcg
     2041 cggctcgcag tgcccgtcga tcgtcagcat cggtcctccc cggggactag gactcgtcgt
     2101 agccggacgg cttgcgatag ccgccgatct cgcgggccag gagcgcggtg aagcggatcg
     2161 tggtcaggtc ctccaggtag ggcccgatcg cctggaggcc gatcggcagc ccctcgcggg
     2221 agaggcccac cgggaacgcg gtggcgggct ggcccggcac cgtggacacc gccggatagc
     2281 agaggccgtg caggtacggg accgaccggc cgttgaccgt gaaggtgagc aagaggtcgc
     2341 tgttgtcgac cggccacgcg cgctcgatgt
//
LOCUS       DATMCV010000768         2695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_910706, whole genome shotgun sequence.
ACCESSION   DATMCV010000768 DATMCV010000000
VERSION     DATMCV010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910706"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..177)
                     /locus_tag="VNU03_17600"
     tRNA            complement(99..177)
                     /locus_tag="VNU03_17600"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(139..141),aa:Glu,seq:ttc)
     gene            complement(266..2374)
                     /locus_tag="VNU03_17605"
     CDS             complement(266..2374)
                     /locus_tag="VNU03_17605"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP76342.1"
                     /translation="MLRLVGGRRVRLYCRLDPAVALTCIGSVGEADSAPAATSADLIF
                     AERVVHERQPLCTPEVGLAAVPVEARGEAVGALLLVGDPGRRFTEHELALLAACADQM
                     ALAVEHAHTQIELARQRYETEELARVARLVNETLDLAAVGKRIASSVLDLLAVPSSAI
                     RLFRPDGSLGAIALGGRARQYGGGSDVVPAGIGLVGRAAAEGRPMWTADFRTDERFET
                     SDEMRRRNETAGIVAGLAVPLRVGGKVIGVLSVGHPTPRTFTTAEIALLQTFADHAAV
                     ALANARTQDDLAKHGERLRVLHEIDRAIITETAPVAIAEAVLWRLRDLLGVPRAIVNL
                     FDWEAGEVEWLAAVGRHRMHLGPGVRYPLNLAGDLAGLRRGEPQIVDVDRLPSTPQAQ
                     ALLASGVHVYMVVPMIVGDDLIGSVSFGGDQAQFSAEQVAIAQEVAAQLAIAITQARL
                     HEQVTRQAAELEHRVAERTRALEMANHELEAFTHTVSHDLKAPLRGMEGFAEALEEDF
                     GDRLDDTGRDFLSRIRASAGRMSRLIDDLLQYSRIEKRPGERGAVALAPLLDELLDEL
                     AGEIRARAISVTADLAVGEVTGEREGLREALANLLSNAVKFSPAQGGTIAVRTYREGG
                     CVVLSVADRGVGFDMQYHDRIFGIFERLHRQEEFPGTGVGLAIVRKVAERHGGRAWAE
                     SEVGRGSTFFLALPDAPAGD"
     gene            complement(2493..>2695)
                     /locus_tag="VNU03_17610"
     CDS             complement(2493..>2695)
                     /locus_tag="VNU03_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76343.1"
                     /translation="GPIPDYAPVHGYADHCRVFSEGELYQRFGHQPNFRLQKIPGAWK
                     HGELPACFEPIEFGSFFVSYSK"
BASE COUNT          394 a          966 c          976 g          359 t
ORIGIN      
        1 cacgtgcgcg ccgttcgagg agaaaggccg gcggggctac cgattcacgg gccagggatc
       61 ctacgcggaa gtactcccgg cgagcctatc aacgcttgtg gtgaccccgg cgggattcga
      121 acccgcgatc tcgaccttga aagggtcgcg tccttggcca ggctagacga cggggtccct
      181 gggcctgccg cagtggctcg gaaggaacag cggcatgata gcaggccctc ggcggggcga
      241 caaccgagcg gccaggtccg ggcgactagt cgccggccgg cgcgtcggga agggccagga
      301 agaaagtgct gccccggccc acctcggact cggcccaggc gcggccgccg tggcgctcgg
      361 ccaccttccg gacgatggcc aggcccacgc cggtaccggg aaactcctcc tggcggtgca
      421 ggcgctcgaa gatgccgaag atgcggtcgt ggtactgcat gtcgaagccg accccgcggt
      481 cggcgaccga gagcacgacg cagccaccct cccggtaggt gcgcactgcg atcgtgccgc
      541 cctgggccgg gctgaacttg accgcgttcg agaggagatt ggcgagcgcc tcacggaggc
      601 cctcgcgctc tccggtcacc tcgcccaccg ccaggtccgc cgtcacgctg atcgcgcgcg
      661 cccggatctc gccggccagc tcgtcgagca gctcgtccag cagcggggcc agagccaccg
      721 cgccacgctc gcccggtcgc ttctcgatgc gcgagtactg gagcagatcg tcgatcagcc
      781 gcgacatgcg cccggccgag gcgcggatcc gcgacaggaa gtcgcgcccg gtatcgtcga
      841 ggcggtcgcc gaagtcctcc tcgagcgcct ccgcgaagcc ttccatcccg cgcaggggcg
      901 ccttgagatc gtgggacacg gtgtgggtga aggcctccag ctcgtggttg gccatctcga
      961 gggcccgggt gcgctcggcg acgcgatgct cgagctcggc ggcctggcgc gtcacctgct
     1021 cgtgcagacg cgcctgcgtg atcgcgatcg cgagctgcgc ggccacctcc tgcgcgatgg
     1081 cgacctgctc cgccgagaac tgcgcctggt cgccgccgaa gctgaccgag ccgatgaggt
     1141 cgtcgcccac gatcatcggc accaccatgt acacgtgcac gcccgaggcg aggagcgcct
     1201 gcgcctgcgg agtcgacggg agccggtcca cgtccacgat ctggggctcg ccccggcgca
     1261 gaccggccag gtccccggcc aggttcaacg gatagcgaac gccgggcccg aggtgcatgc
     1321 ggtggcgacc caccgcggcg agccactcca cttccccggc ctcccaatcg aacaggttga
     1381 cgatggcgcg gggcacgccc agcaggtcgc gcaaccgcca gagcaccgcc tcggcgatcg
     1441 cgaccggcgc ggtctcggtg atgatcgcgc gatcgatctc gtgcaggacc cgcagacgct
     1501 cgccgtgctt ggccaggtcg tcctgggtgc gcgcgttcgc cagggcgacc gcggcgtgat
     1561 cggcgaacgt ctgcagcagc gcgatctcgg cggtggtgaa ggtccggggc gtggggtggc
     1621 cgaccgagag cacgccgatc accttgccgc cgacccgcag cggcaccgcg agcccggcca
     1681 cgataccggc cgtttcgttg cgtcgcctca tctcgtcgct ggtctcgaac cgctcgtcgg
     1741 tccggaagtc ggccgtccac atcgggcggc cctcggcggc cgcgcgaccc acgaggccga
     1801 tcccggccgg cacgacgtcg ctgccgccgc cgtattgccg ggcgcggccg cccagcgcga
     1861 tcgcacctag gctcccgtcg gggcgaaaga gccggatggc ggagctcggg acggccagca
     1921 ggtcgagcac gctcgacgcg atccgcttgc ccaccgcggc caggtcgagc gtctcgttga
     1981 ccagccgggc cacgcgggcc agctcctccg tctcgtagcg ctggcgcgcc agctcgatct
     2041 gggtatgggc gtgctcgacc gcgagcgcca tctggtcggc gcaggcggcc agcagggcca
     2101 gctcgtgctc cgtgaagcgg cggcccgggt cgccgacgag cagcagggcc ccgaccgcct
     2161 ctccgcgcgc ctcgacggga accgcggcca gccccacctc gggcgtgcag aggggttggc
     2221 gctcgtggac gacgcgctcg gcgaagatca ggtccgcgct cgtggccgcg ggagcggaat
     2281 cggcctcccc cacggagccg atgcacgtga gagcgacggc gggatcgaga cggcagtaga
     2341 ggcggacgcg gcggcctccg acgagccgga gcaccgagct cgcaattcgc tccgcgacgg
     2401 ggcccacgtc gggggcgcct gccggtggca cgtgccggga aggctgcccc atacctccag
     2461 ggtacctcct gcccgatttt ccacctggag ggctatttcg agtaggagac gaagaaggag
     2521 ccgaactcga tcggctcgaa gcaggcgggc agctcgccgt gcttccacgc gcccgggatc
     2581 ttctgcaggc ggaagttggg ctggtgcccg aagcgctggt acagctcgcc ctcgctgaag
     2641 acccggcagt ggtcggcgta gccgtggacc ggggcgtagt ccgggatggg gccgc
//
LOCUS       DATMCV010000769         2906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_910863, whole genome shotgun sequence.
ACCESSION   DATMCV010000769 DATMCV010000000
VERSION     DATMCV010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910863"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1127)
                     /locus_tag="VNU03_17615"
     CDS             complement(105..1127)
                     /locus_tag="VNU03_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWP76344.1"
                     /translation="MITATRSPLHTPLCDRLGIRYPICQAGMAFVARAQLAAAVSAAG
                     GLGVIAAAHGSPALLREEIRRVRELTDRPFGVDILFATIRTSGRETEAFTDAVRGWCD
                     VALEERVPVLIAGLGNPGPLTAEAHRLGMTVMALCGNVKQARDHAANGVDVIIAQGHE
                     GGGHTGRVGGVVLTPAVVDAVAPRLVVAAGGIADGRGLVAALALGAAGVWMGTRFIAT
                     AEAHGHDNYKQKIAAIDEEGTVVTRAVSGKPNRQIRNAFTREWEGRQAEIEPFPIQYE
                     RVGKPASIRAREEGDVEHGSAAAGQSAGLVRDVRPAGQVVASIVEEAEAILQMLGRTP
                     GARGEA"
     gene            complement(1183..1269)
                     /locus_tag="VNU03_17620"
     tRNA            complement(1183..1269)
                     /locus_tag="VNU03_17620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1233..1235),aa:Leu,seq:caa)
     gene            1378..1908
                     /gene="tpx"
                     /locus_tag="VNU03_17625"
     CDS             1378..1908
                     /gene="tpx"
                     /locus_tag="VNU03_17625"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HWP76345.1"
                     /translation="MADRAGAVMFKGNPIALGGPEIKAGSDAPDFTALDNGLQPVKLS
                     QARGKVIVLSAVPSLDTPVCDTETRKFNVEAGKLGGNVEVWTVSMDLPFAQKRWCGAA
                     GVTAVKTLSDFRERSFAQNYGVLVKEGPLAGLTARAVFVVGKDGKVKHAEYVKEITTE
                     PNYDAALAAAKQAAAG"
     gene            1984..2059
                     /locus_tag="VNU03_17630"
     tRNA            1984..2059
                     /locus_tag="VNU03_17630"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2017..2019,aa:Ala,seq:ggc)
     gene            complement(2139..2537)
                     /locus_tag="VNU03_17635"
     CDS             complement(2139..2537)
                     /locus_tag="VNU03_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HWP76346.1"
                     /translation="MVEPRPVPGVVHLRSQAPAAATLDRLEALVRARGLMVFARIEFS
                     RDAAAVGLPLPPMAQLVFGNSRAGTPLLAASPAIGLALPLRALAWEDGEGHSWLSYEV
                     PESLAERFGVPAGLAGNIGAIRSLCEAAVG"
     gene            complement(2589..>2906)
                     /locus_tag="VNU03_17640"
     CDS             complement(2589..>2906)
                     /locus_tag="VNU03_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWP76347.1"
                     /translation="SEFAMYGLMFVLPLVGWGMLSAERYPIVLFGSVHLPFILPPSAR
                     LYSFLRSAHTILAYAFALTFLAHFGAILFHTLIVRDGLLTRMAPWNTRPPGVASADPT
                     ARE"
BASE COUNT          471 a         1059 c          994 g          382 t
ORIGIN      
        1 gatcacgcag tcggcgccct cgcgcgccag gtacagcgcg atgccgcggc cgatgccacg
       61 cgagccgccg gtgacgaccg cgacccgccc cgtgagcgga ccgctcacgc ttcgccccgc
      121 gcgccaggcg tccgccccaa catctggagg atcgcctcag cctcctccac gatcgacgcg
      181 accacctggc cggccgggcg cacgtcgcga accaggccgg ccgactgccc ggccgccgcg
      241 ctgccgtgct cgacgtcgcc ctcctcccgc gcccggatcg aggcgggctt gcccacgcgc
      301 tcatactgga tcgggaacgg ctcgatctcg gcctgccggc cctcccactc gcgggtgaac
      361 gcgttgcgga tctggcggtt gggcttaccg gacaccgcgc gggtcaccac cgtgccctcc
      421 tcgtcgatgg ccgcgatctt ctgcttgtag ttgtcgtggc cgtgcgcctc ggcggtggcg
      481 atgaagcgcg tgcccatcca gacgccggcc gcccccagcg ccagcgcggc cacgagcccg
      541 cgcccgtcgg cgatcccgcc cgccgccacc acgaggcggg gcgccaccgc gtccaccacc
      601 gccggcgtga gcacgacgcc gcccacgcgc ccggtgtgtc cgccgccctc gtggccctgc
      661 gcgatgatca cgtccacccc gttggccgcg tggtcgcgcg cctgcttcac gttgccgcag
      721 agggccatca cggtcatgcc gaggcggtgc gcctccgcgg tgagcggtcc cgggttgccg
      781 agccccgcga tcagcaccgg cacgcgctcc tcgagcgcca cgtcgcacca gccgcggacc
      841 gcgtcggtga acgcctccgt ctctcgaccg ctggtccgga tggtggcgaa gaggatgtcg
      901 acgccgaagg gccgatcggt cagctcgcgc acccggcgga tctcctcgcg cagcagcgcg
      961 gggcttccgt gcgcggctgc gatgaccccg agcccacccg ccgcggatac cgcggccgcg
     1021 agctgggcgc gcgccacgaa cgccatgccg gcctggcaga tcggatagcg gatgccgagc
     1081 cggtcgcaga gcggagtgtg gagcggggag cgcgtcgccg tgatcatgcc gctcacctcc
     1141 cgctcccatc gaggacgcga tcagccgagc gcccggaaaa agtggtgccg gaggcgggac
     1201 tcgaacccgc acatccttgc ggacaccgca ttttgagtgc ggcgcgtatg ccagtttcgc
     1261 cactccggcc cgtggctcgc ttcagctcga gcctatgata cgacaaccct agcttgtata
     1321 ctgtggagcg ctcgcggtaa gcgtcccgac ccgaattcca tccaacggag gtgcgccatg
     1381 gccgatagag caggcgccgt gatgttcaaa ggcaatccga tcgcgctcgg cggtccggag
     1441 atcaaggcgg gcagcgacgc ccccgacttc accgcgctcg acaacgggct ccagcccgtg
     1501 aagctgagcc aggcccgcgg caaggtcatc gtgctcagcg cggtgccgtc gctcgacacc
     1561 ccggtgtgcg acaccgagac ccgcaagttc aacgtggagg cgggcaagct cggcggcaac
     1621 gtcgaggtgt ggacggtgag catggacctg cccttcgcgc agaagcgctg gtgcggcgcg
     1681 gccggggtca ccgcggtgaa gaccctgtcc gacttccgcg agcgctcctt cgcccagaac
     1741 tacggcgtgc tcgtgaagga gggccccctc gccgggctga ccgcgcgcgc ggttttcgtg
     1801 gtgggcaagg acggcaaggt caagcacgcg gagtacgtca aggagatcac caccgagccg
     1861 aactacgacg cggccctcgc ggcggcgaag caggcggccg ccggctaggt cgggttgggt
     1921 cgtcgcaagt attggtcaac cttgacaaaa gaaagccagc cacgttaggc tgaacggtgc
     1981 ggtggggctg tagctcagtt gggagagcac aaggctggca gtcttggggt caggggttcg
     2041 agccccctca gctccaccaa aaattccaga gtcaccgaca cggggcaggc gatgccagat
     2101 ccggatcgcc tgccccgtcg tccttccggt cccgcgggct acccgaccgc cgcctcgcag
     2161 agtgaccgga tcgcgccgat gttgcccgcg agcccggcgg gcacgccgaa gcgctccgcc
     2221 agggactccg gcacttcgta gctcaaccag ctgtggccct cgccgtcctc ccacgcgagc
     2281 gctcgcagcg gcagcgcgag gccgatggcc gggctggcag ccagcagagg ggtcccggcg
     2341 cgagagttgc cgaacacgag ctgcgccatc ggggggagcg ggagcccgac cgccgccgca
     2401 tcgcggctga actcgattcg agcgaacacc atcaggccac gtgcgcgcac cagcgcctcg
     2461 agtcgatcca gcgtcgccgc cgccggggcc tgactgcgca ggtgcaccac gccaggaacc
     2521 ggacgcggct ccaccatgac gaaccctcct tgcgaccgag tcccggagcc gcccgccggc
     2581 cggcgctcct actcgcgagc ggtgggatcg gcggatgcga cccccggtgg ccgggtgttc
     2641 cacggcgcca tccgcgtcag gaggccgtcc cgcacgatca gggtgtgaaa caagatcgcc
     2701 ccgaagtgag ccaggaacgt caaggcaaac gcgtaggcga ggatggtgtg cgccgagcgc
     2761 aggaaagaat acagtcgcgc actcggcgga aggatgaacg gcaggtgcac ggagccgaag
     2821 aggacgatcg gatagcgctc cgcagacagc atcccccagc cgaccagcgg gagaacgaac
     2881 atcaggccat acatcgcgaa ttccga
//
LOCUS       DATMCV010000770         2209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_377493, whole genome shotgun sequence.
ACCESSION   DATMCV010000770 DATMCV010000000
VERSION     DATMCV010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377493"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1105)
                     /gene="cofD"
                     /locus_tag="VNU03_17645"
     CDS             complement(161..1105)
                     /gene="cofD"
                     /locus_tag="VNU03_17645"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HWP76348.1"
                     /translation="MKITALAGGTGAAKLLRGLGRVLDPRDLTVVVNTGDDTEIWGLH
                     VSPDLDTVCYTLGGVIDERKGWGLTDETFHALDQIARYGEPVWFNLGDRDLATHLHRS
                     RLLREGRSLTQITRAIAQALGVTATVLPMSDQPVRTRILGPDGWLAFQEYFVREKAQV
                     EVRAVNYAGAPAARPAPGVLDALRTADAVLLCPSNPITSIGPILAVPGLVEALQSTEA
                     IVAAVSPIVGGDAVSGPAGRLMAAAGLPVSATGVARAYGGWLDLLVFDEQDRTLAPEI
                     RSAGAAPVVAPTMMSSRDAEVTLARHVVAALEAARAAR"
     gene            complement(1122..1475)
                     /locus_tag="VNU03_17650"
     CDS             complement(1122..1475)
                     /locus_tag="VNU03_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76349.1"
                     /translation="MGALLMERAGGAAMLHGPVVVAPPGTPAEDVLGVAADLTAEALA
                     LAAPGGIETIFARPQSLDRVWVRTGFIPVPEAELPRPLRGRPGLGLFGWRGGTALWST
                     AGRGGSRSALARGEH"
     gene            complement(1615..1902)
                     /locus_tag="VNU03_17655"
     CDS             complement(1615..1902)
                     /locus_tag="VNU03_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWP76350.1"
                     /translation="MAKSMTKSAIIAHLAQKTELSKKQVVDVMDQLLSLAQKEAKNVF
                     VLPNFGRLVLANRKARMGRNPQTGEPIKIPAKRVVKFRLAKSLKDTVLGKK"
BASE COUNT          316 a          755 c          838 g          300 t
ORIGIN      
        1 gtgatcacca ccaccgagcc ggggagcgcc gccaccagcg cttcgagcac gtcgcccagc
       61 atggcgcgcg cgagctcgcg ccgctcggcg ggcgagaggg cgggtacgag ccgctgcttg
      121 gcgttcacga ggtccttgac cggcacggcc gcgatcacgc tcatcgcgcg gcccgcgctg
      181 cctcgagcgc ggcgaccacg tgccgagcca gcgtcacctc ggcgtcgcga ctcgacatca
      241 tggtgggggc cacgaccggg gccgctccgg cggagcgaat ctccggggcc agcgtccggt
      301 cctgctcgtc gaagaccagg aggtcgagcc agccgccgta ggcgcgcgcc acgccggtgg
      361 ccgagacggg caggccggcc gcggccatga gccggccggc gggacccgac accgcgtcgc
      421 cgcccacgat cgggctgacc gcggccacga tcgcctcggt ggactgcagc gcctcgacga
      481 gcccgggcac ggccaggatc gggccgatgg aggtgatcgg gttcgagggg cacagcagga
      541 ccgcgtcggc ggtgcggagt gcgtccagga cgccgggggc gggccgagcg gccggcgccc
      601 ccgcgtagtt caccgcgcgc acctcgacct gggccttctc gcgcacgaag tactcctgga
      661 acgcgagcca gccgtcgggg ccgaggatcc gggtgcgcac cggctgatcg gacatgggga
      721 gcaccgtggc ggtgactccc agggcctggg cgatcgcccg cgtgatctgg gtgagggagc
      781 ggccctcgcg gaggaggcgg ctgcggtgga gatgggtcgc caggtcgcgg tcgcccaggt
      841 tgaaccagac cggctcaccg tagcgcgcga tctggtcgag ggcgtggaag gtctcgtcgg
      901 tgaggcccca gcccttgcgc tcgtcgatga cgccgcccag ggtgtagcag acggtatcga
      961 ggtccggcga gacgtgcagg ccccagatct cggtatcgtc cccggtgttg acgacgacgg
     1021 tgagatcgcg cggatcgagg acgcggccga gaccgcggag cagcttggcg gcgccggtgc
     1081 cgcccgcgag cgcggtgatc ttcatgccgc ggctccgccc gtcagtgttc gccgcgggcg
     1141 agggcggagc gggagccgcc gcgaccggcg gtgctccaga gcgcggtgcc gccccgccag
     1201 ccgaagaggc cgaggccggg ccggccgcga agcgggcggg gcagctccgc ttccggaacc
     1261 gggatgaagc cggtgcgcac ccagacccgg tccaggctct gggggcgcgc gaagatggtt
     1321 tcgatgccgc cgggagcggc gagggccagc gcctcggcgg tgaggtcggc ggccacaccc
     1381 agcacgtcct cggcgggcgt gccgggcggg gcgaccacga ccggaccgtg caacatcgcg
     1441 gcgccgcccg cccgctccat caggagagct cccacgagcc gctccccttc ccaggcgccc
     1501 cagccgacgg aaatgtcgtc cggcggtgga gcccatgcgc ctgcaaagcg gaagagctcg
     1561 agggccgcct cccatggtgc ggcgagcggc cggtaagcgg gcgcggggcc cgtgctactt
     1621 cttgccaagc accgtgtcct tgagggactt ggcgagccgg aacttcacca cgcgcttggc
     1681 gggaatcttg atgggctctc cggtctgcgg gttgcgcccc atccgcgcct tgcgattcgc
     1741 cagcacgagc cggccgaagt tcggcagcac gaatacgttc ttggcttcct tctgggcgag
     1801 cgagagcagc tggtccatca cgtcgacgac ctgcttcttg ctgagctccg tcttctgggc
     1861 caggtgagcg atgatggcgg acttcgtcat cgatttcgcc atgtgcgttc ctccgttgtg
     1921 ctggatgtga gagtgaaggg ccggaccgtc taggccgacc gcttaggatg gtcgcgtccc
     1981 gcgtgaaacc aaggcccaat tctactcaag gcgcctcgcg cgctcaacag ccgaatgcgt
     2041 ccgtccgcgg ctcaggcgtc cgggacgcgc gtgggatgaa ggaagcggaa cttgccggag
     2101 gaggccagca tccggtacga gccgaagcgg gcctccgcgt tctcgacgtg gtcgagcggg
     2161 gagtcgtccg cggcgtcctc cacctcgtag acgcggagca ggtcgtagg
//
LOCUS       DATMCV010000771         4468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_107709, whole genome shotgun sequence.
ACCESSION   DATMCV010000771 DATMCV010000000
VERSION     DATMCV010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107709"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VNU03_17660"
     CDS             complement(<1..337)
                     /locus_tag="VNU03_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWP76351.1"
                     /translation="MASKLLGAHVKRKEDPRLITGTSQYVGDVAMPGMQHVAFVRSPH
                     AHARVRGIKSGAALKVPGVVGVVTGQELKPHVSPIPGVQASAEGGEVTEAKVGRQHYP
                     LSVDRVRYVG"
     gene            complement(354..962)
                     /locus_tag="VNU03_17665"
     CDS             complement(354..962)
                     /locus_tag="VNU03_17665"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HWP76352.1"
                     /translation="MSPDSDARPTLTDADLDLILRKARTYRAWLDRPVSDDLLRRVYE
                     LAAQGPTSANCSPMRVLFARSREAKERLRPALTPGNVDKTMQAPVTAIIGHDMDFYEQ
                     LPRLFPTADMRANFVGKPELIAATAFRNGTLQGAYLMLAARALGLDCGAMSGFDNAKV
                     DAEFFPDGRVKSNFLCNLGYGDASKLGPRPPRQPFDEACRFI"
     gene            1100..>4468
                     /locus_tag="VNU03_17670"
     CDS             1100..>4468
                     /locus_tag="VNU03_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP76353.1"
                     /translation="MPPGTGPRKARRKTHVKTKPAAGKARGPRPTLPGSPEARLLEAL
                     EQQAATSEILRVISSSPTDAQPVFDAIVRSAARLCGAEFSAVARFADGLLHLVAVNNL
                     SPEEAEAFRLLFPRAPGRGFAMGRAFVDGRPAHVEDVFADSDYDRQTQGLLQRVTGYR
                     TFLGVPILRAGVPVGVIGCARRSVQPFTALQIDLVKTFAEQAVIAIENVRLFQELEQR
                     NRALTGSLEQQTATGEILRVISSSPTDVRPVLDTVSESAARLCEAFDAAIWRRDGDRL
                     LLVAHHGPILQGPVGQFSLPLAGTAAGRSVLEARTVHVADLLADPDEFPDTSENARRT
                     GFRTVLCVPLMREGTAIGTITLPRTEVQLFSESQIALLETFADQAVIAIENVRLFTEL
                     EGRNRALTEALDQQTATSEILRVISSSPTDIRPVLDTVAESAARLCDATDAAIFREDG
                     GRLRMVAHHGPIPFGTVGEYTIQLVRGTVLGRTVLDGRTVQVDDLLSASDEFPEGQEL
                     ARELGHRTTLAVPLLREGVAIGAITIRRTEVRPFTDRQIALLETFADQAVIAIENVRL
                     FTEVQDRNRELTDTLEQQTATGEILRVISGSPTDVQPVFDTIARNARRLCEADSAAVF
                     TYDGELVHLQSLANVGFAGADALRQAYPAPVNDGHATGRAIRQGRPVHIEDAQVDRDF
                     NLTGVLGAGVRTVLAVPMLRDGAAVGIIVLHTWTTPRPFSESQIALLETFADQAVIAI
                     QNVRLFTEVQERNHDLTETLEQQTATGEILRVISSSPTDVQPVFDAIAHSAARLCEAE
                     MCFVFRFDGEILHFVAHHGLGPAALAAAGAAWPAPPTRGSAAGRAILERGVAHIPDAQ
                     TDPDYALTALASVAALRGALAVPMMREGQPVGVISVARSQVGHFTARQIALLQTFADQ
                     AVIAIQNVRLFKELEVRNHDLIETLEQQTATSEILRVISSSPTDIQPVLDTVAEYAAK
                     VCGAQNSVVWRLEGDRLTRAASHGPIPIGGNHLDVAPGSVTGRAVLEGRAIHSHDLQN
                     DPEFPVGREIARRTGHRTTLATPMLREGTPIGAILIRRLEVQPFTEKQIALLQTFADQ
                     AVIAIQNVRLFTEVQERNHDLTETLEQQTATGEIL"
BASE COUNT          645 a         1663 c         1522 g          638 t
ORIGIN      
        1 cgcccacgta gcgcacccgg tccaccgaga gcgggtagtg ctggcggccg accttggcct
       61 cggtgacctc gccgccttcc gccgaggcct gcacgcccgg gatcgggctc acgtgcggct
      121 tgagctcctg gccggtgacc accccgacca ccccgggcac cttgagcgcg gcgccgctct
      181 tgatcccgcg gacccgcgcg tgggcgtgcg ggctccgcac gaacgccacg tgctgcatgc
      241 cgggcatcgc cacgtcgccc acgtactgcg aggtgccggt gatgagccgc gggtcttcct
      301 tgcgcttgac gtgtgcgccc aggagcttgg aggccatggt cggatctcct ctgctagatg
      361 aagcggcagg cttcgtcgaa gggctgtcgc gggggccggg gcccgagctt gctcgcatcg
      421 ccgtagccga gattgcacag gaagttggac ttcacgcgcc cgtccgggaa gaactccgcg
      481 tccaccttgg cgttgtcgaa gccggacatg gcgccgcagt cgagcccgag cgcgcgggcg
      541 gccagcatca ggtaggcgcc ctggagggtg ccgttgcgga acgcggtcgc cgcgatcagc
      601 tccggcttgc ccacgaagtt ggcccgcatg tcggcggtgg gaaagagccg cgggagctgc
      661 tcgtagaagt ccatgtcgtg cccgatgatc gcggtgaccg gggcctgcat ggtcttgtcg
      721 acgttgcccg gggtcagggc cggccgcaac cgttccttgg cctcgcgcga cctcgcgaag
      781 agcacgcgca tcgggctgca gttcgcgctg gtgggcccct gcgcggccag ctcgtacacc
      841 cgacgcagga ggtcgtcgga gacgggacgg tcgagccagg cccggtacgt gcgggccttg
      901 cgcaagatca gatcgaggtc ggcgtcggtg agcgtgggcc gcgcgtcgga atcggggctc
      961 atgaccgtcg agtatcgggg ctcccagcga ccgcgtcaag cgaccggccc gcttggccct
     1021 tgttcagccg ggcgccatcg cttagcctgt cgtccactct cgcgctcttg cgggagcgat
     1081 cccaggtcca ggagagccga tgcccccagg taccgggccg aggaaggccc gccggaagac
     1141 tcacgtcaag accaagcccg ccgccggcaa ggcgcgcggg ccccgtccca cccttcccgg
     1201 gagccccgaa gcacgtttgc tcgaggctct cgagcagcag gccgccacca gcgagatcct
     1261 gcgggtcatc tcgagctccc ccaccgatgc ccagccggtc ttcgacgcca tcgtgcggag
     1321 cgcggcgcgc ctgtgcggcg cggagttcag cgcggtggcg cgcttcgcgg atggcctcct
     1381 tcacctggtg gcggtcaaca acctctcgcc ggaggaggcg gaggccttcc ggctcctgtt
     1441 cccgcgagcc cccgggcgcg gcttcgccat gggccgcgcc ttcgtggacg gccggcccgc
     1501 gcacgtggag gacgtcttcg cggattccga ctacgaccgt cagacccagg gtctcctcca
     1561 gcgcgtcacc ggttaccgga cgttcctggg ggtcccgatc ctccgggccg gggttccggt
     1621 cggagtgatc gggtgcgccc ggcggtcggt gcagccgttc accgccctcc agatcgacct
     1681 cgtcaagacc ttcgcggagc aggcggtgat cgccatcgag aacgtgcgcc tcttccagga
     1741 gctcgagcag cgcaatcggg ccctgaccgg gtccctcgag cagcagaccg cgaccggcga
     1801 gatcctccgc gtcatctcga gctcgcccac cgacgtccgg ccggtgctcg acacggtgtc
     1861 ggagagcgcg gcgcgcctgt gcgaggcgtt cgacgccgcg atctggcgtc gcgacggcga
     1921 ccggctcctc ctcgtcgcgc atcacgggcc gatcctccag gggccggtcg gccagttctc
     1981 ccttcccctc gccggcaccg ccgcgggccg gtcggtgctg gaggcgcgga ccgtccacgt
     2041 cgccgacctg ctcgcggacc cggacgagtt cccagatacc agcgagaacg cgcgccgcac
     2101 cggcttccgc accgtcctct gcgttcccct gatgcgggag gggaccgcga tcggtacgat
     2161 caccctcccc cgcaccgagg tccagctctt ctccgagagc cagatcgcgc tcctcgagac
     2221 cttcgccgac caggcggtca tcgcgatcga gaacgtgcgc ctgttcaccg agctcgaggg
     2281 ccgcaaccgg gccctcaccg aggccctcga ccagcagacc gccacgagcg agatcctccg
     2341 cgtgatctcg agctcgccca ccgacatccg gccggtgctc gacacggtgg ccgagagcgc
     2401 ggcgcgcctc tgcgacgcca ccgacgcggc catcttccgc gaggacggcg gccggctgcg
     2461 catggtcgcc caccacggcc cgatcccctt cggcaccgtg ggggagtaca cgatccagct
     2521 cgtgcggggc accgtcctcg gccggaccgt gctcgacggg cgcaccgtcc aggtcgacga
     2581 cctcctgagc gcgtccgacg agttccccga gggacaggag ctggcgcggg agctcggcca
     2641 tcggacgacc ctcgcggtgc ccctgctccg agaaggcgtg gcgatcgggg cgatcaccat
     2701 ccgccggacc gaggtgcggc cgttcaccga ccgccagatc gccctcctcg agacattcgc
     2761 ggaccaggcg gtgatcgcga tcgagaacgt gcgcctgttc accgaggtcc aggaccgcaa
     2821 ccgcgagctg accgacacgc tggagcagca gaccgccacc ggcgagatcc tgcgggtcat
     2881 ctccggctcg cccaccgacg tgcagccggt gttcgacacc atcgcgcgta acgcccggcg
     2941 cctctgcgag gccgactccg ccgcggtgtt cacctacgac ggcgagctgg tccacctcca
     3001 gtcgctcgcc aacgtgggat ttgccggcgc ggacgcgctc cggcaggcct accccgcccc
     3061 ggtgaacgac gggcacgcga ccgggcgggc gatccggcag ggccgcccgg tccacatcga
     3121 ggacgcgcag gtcgaccgcg acttcaacct caccggagtg ctgggggccg gggtgcgcac
     3181 cgtcctcgcg gtcccgatgc tgcgcgacgg ggccgcggtc ggcatcatcg tgctccacac
     3241 ctggaccacg ccccgtccat tctccgagag ccagatcgcg ctgctcgaga ccttcgcgga
     3301 tcaggcggtc atcgcgatcc agaacgtccg cctgttcacc gaggtgcagg agcgcaacca
     3361 cgatctgacc gagaccctcg agcagcagac cgcgaccggc gagatcctgc gggtcatctc
     3421 gagctcgccc accgacgtcc agccggtctt cgacgcgatc gcgcacagcg cggcccggct
     3481 ctgcgaggcc gagatgtgct tcgtcttccg cttcgacggg gagatcctgc acttcgtggc
     3541 ccatcacggg ctggggcccg cggcgctcgc ggcggccggg gccgcgtggc ccgccccgcc
     3601 cacccgcggc tccgcggccg gccgcgcaat cctggagcga ggcgtcgccc acatcccgga
     3661 cgcccagacc gaccccgact acgccctgac cgcgctcgcg tccgtcgcag ccctccgcgg
     3721 agcgctcgcg gtgccgatga tgcgcgaggg ccagccggtc ggggtcatca gcgtcgcgcg
     3781 ctcccaggtc ggtcacttca ctgcccggca aatcgctctg ctgcagacct tcgccgacca
     3841 ggcggtcatc gcgatccaga acgtgcggct cttcaaggag ctcgaggtcc gcaaccacga
     3901 cctcatcgag accctggagc agcagaccgc caccagcgag atcctccggg tgatctcgag
     3961 ctcgccgacg gacatccagc cggtcctgga cacggtggcc gagtacgccg cgaaggtgtg
     4021 cggggctcag aattcggtgg tatggcggct cgagggcgac cgcctgacgc gcgccgcgtc
     4081 acacgggccg attcccatcg gggggaatca cctggatgtg gcgccgggtt cggtcaccgg
     4141 acgcgcggtg ctcgaggggc gggccatcca ctctcacgac ctgcagaacg atccggagtt
     4201 cccggtcggc cgggagatcg cccgccgcac cggtcaccgc acgacgctcg cgacgccgat
     4261 gctccgggaa gggacgccga tcggggcgat cctcatccgg cgcctggagg tgcagccgtt
     4321 caccgagaag cagatcgccc tgctccagac cttcgcggac caggcggtca tcgcgatcca
     4381 gaacgtccgc ctcttcaccg aggtgcagga gcgcaaccac gatctgaccg agaccctcga
     4441 gcagcagacc gcgaccggcg agatcctg
//
LOCUS       DATMCV010000772         4595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_240329, whole genome shotgun sequence.
ACCESSION   DATMCV010000772 DATMCV010000000
VERSION     DATMCV010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240329"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..616)
                     /locus_tag="VNU03_17675"
     CDS             complement(8..616)
                     /locus_tag="VNU03_17675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76354.1"
                     /translation="MRRPLGLLLALLLAGAAAAPAARAADAATITWGVHVSLAPTFFD
                     PAEATGTALPLMVYYAMHDALVRPLPGQAMGAALATSWTVSRDGLVYEFVLRRGVVFH
                     NGDPFTAEDVKFSFERYRGASATQLKAKVAAVEIVDPYRVRFRLKQPWPDFLTFYATP
                     PPGRPGSCRRSTSRRWATRASRGRRWGRGPTSSSRSSPGSSW"
     gene            complement(613..2070)
                     /locus_tag="VNU03_17680"
     CDS             complement(613..2070)
                     /locus_tag="VNU03_17680"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76355.1"
                     /translation="MRTVIEGGWVVAWNGRGHEVHEQGSVVFEDHRVVHAGGPYTGAV
                     DARISARGKLVSPGFINTHVHTAGNGGDYLLLDMAKNDYRTANYMAFAAPLKGKMTPP
                     PAEATAALRAFVFLHALKNGTTTVIDVGGLRGDWDGYARLVDDLGVRVYGGPPFRDRD
                     TYMDTQGRLTYEEDVAVGRARLQEAVDFARKFDGVAQGRLRALFNAAQVETCSEPLLR
                     AGKDAARAIGAPIHTHAGGNLVEFQRIMDEYRKTPVRFLADIDFLDERTLLGHVVFTT
                     AHEWAHYPFGDDLRLLAEHGSTVGHCPYKYAKMAMTLRSFQRYLEAGVTLALGTDTFP
                     LDIVSELRWASILAKVTDANYQAGLARDVFNAATIGGCRFLGREDLGRLAPGAKADIL
                     LIDLDHAGGPVYADPIKALVDSASGRDVDTVIVDGQVLVQGGRATRVDEDAVYAKARE
                     ATQHYWSRVSGWRWDGATVDQIVPPAFPIHRAARA"
     gene            complement(2561..3502)
                     /locus_tag="VNU03_17685"
     CDS             complement(2561..3502)
                     /locus_tag="VNU03_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWP76356.1"
                     /translation="MRLVTFRRGRGEPKPGALWQEHVLDLGGLAKDLAVQRGTVRRGR
                     GGFPRTILEIVQGSDAVRALAREAVEHGQRLVGQTAAEELAQRRLAYPLKRVRLLAPI
                     PSPSRNIFCLGRNYADHAAERGAAAPEHPVYFTKPATAVVGPGDDVVHHAITKELDYE
                     VELTVVIGTGGRDIARADALKHVFGYTIINDVTARDLQKKHQQWFKGKSLDTFCPMGP
                     VLVTADELPDPQALAISLRVNGQLRQSSHTSKMIFPVAQCIEVLSQGMTLLPGDIIAT
                     GTPEGVGAASGNFLKAGDRIEAEVESIGILHSKVVRP"
     gene            complement(3506..4507)
                     /gene="ispH"
                     /locus_tag="VNU03_17690"
                     /gene_synonym="lytB"
     CDS             complement(3506..4507)
                     /gene="ispH"
                     /locus_tag="VNU03_17690"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWP76357.1"
                     /translation="MTTLPLQQIVLAGPRGFCAGVDRAIDIVELALQVCPPPVYVRKE
                     IVHNRHVVEALRAKGAHFVDELDEVPDDATVIFSAHGISPAVRAEAQRRGLRVIDATC
                     PLVTKVHLEAVRYAREGYSIILVGHADHDEVVGTMGEAPDRMFLIAHPEDVDRLAVAD
                     PDKVAYLTQTTLSVDDTRDCIEALRRRFPKIVGPAKDDICYATQNRQAAVKTVAGAVD
                     VLLVIGAANSSNANRLVEVSTVMGTPSYLINDKNDIRPEWLEGLSRVGVTAGASTPEH
                     LVTEVVEELRRKHGSSVQEVHVVEEDVRFGLPRELEDFARAAGKTLPPRAGAVAVSG"
BASE COUNT          634 a         1642 c         1646 g          673 t
ORIGIN      
        1 gcctccatca ccagctcgac cccgggcttg aacgcgacga acttgtaggg ccccgccccc
       61 accggcgccc tcttgaagcc ctcgtcgccc accttctcga cgtacttctt cggcacgatc
      121 caggccgccc cggtggcggg gtggcgtaga acgtgaggaa gtcgggccac ggctgcttga
      181 gccggaagcg cacccggtag ggatcgacga tctccaccgc ggccaccttg gccttgagtt
      241 gggtcgcgct cgccccgcgg tagcgctcga aggagaactt cacgtcctcc gcggtgaacg
      301 ggtcgccgtt gtggaagacg acgccccggc gcagcacgaa ctcgtagacc agcccgtcgc
      361 ggctgaccgt ccacgacgtc gccagcgcgg ctcccatcgc ctggccgggc agcgggcgca
      421 ccagcgcgtc gtgcatcgcg tagtagacca tcagcggcag ggcggtgccg gtggcctcgg
      481 cgggatcgaa gaaggtgggc gcgagcgaga cgtgcacgcc ccacgtgatg gtggcggcgt
      541 cggccgcgcg cgcggcaggc gccgccgccg cgccggccag gagcagcgcg aggaggagcc
      601 cgagcggccg cctcacgcgc gcgcggcccg gtggatgggg aacgcgggcg ggacgatctg
      661 gtccaccgtc gcgccgtccc agcgccaccc ggacacccgg ctccagtagt gctgggtggc
      721 ctcgcgcgcc ttggcgtaca ccgcgtcctc gtccacccgg gtcgcgcggc cgccctgcac
      781 cagcacctga ccgtccacga tgacggtgtc cacgtcgcgc cccgaggccg agtcgaccag
      841 cgccttgatc gggtccgcgt agaccggccc gcccgcgtgg tcgaggtcga tcagcaggat
      901 gtcggccttg gctccggggg ccaggcggcc gaggtcctcg cggccgagga accggcagcc
      961 gccgatggtc gcggcgttga acacgtcgcg cgcgagcccc gcctggtagt tggcgtcggt
     1021 gaccttggcc aggatggagg cccagcgcag ctcggacacg atgtcgagcg ggaaggtgtc
     1081 ggtgccgagg gcgagggtga ccccggcctc gaggtagcgc tggaaggagc gcagggtcat
     1141 cgccatcttc gcgtacttgt acgggcagtg gccgacggtg ctcccgtgct cggcgagcag
     1201 gcgcaggtcg tcgccgaacg gatagtgcgc ccactcgtgc gcggtggtga agaccacgtg
     1261 gcccagcagg gtgcgctcgt cgaggaagtc gatgtcggcg aggaagcgca ccggggtctt
     1321 gcggtactcg tccatgatcc gctggaactc gacgaggttg ccgcccgcgt gggtgtggat
     1381 cggcgccccg atcgcgcgcg ccgcgtcctt gcccgcccgc agcagcggct cgctgcaggt
     1441 ctcgacctgg gccgcgttga agagcgcacg caggcggccc tgcgcgaccc cgtcgaactt
     1501 gcgggcgaag tccaccgcct cctggagccg tgcgcgaccc accgcgacgt cctcctcgta
     1561 ggtgagccgg ccctgcgtgt ccatgtaggt gtcgcggtcg cggaacggcg gcccgccgta
     1621 gacgcgcacc ccgaggtcgt ccaccagccg cgcgtagccg tcccagtccc cgcgcaggcc
     1681 gcccacgtcg atcacggtgg tggtgccgtt cttcagcgcg tggaggaaga cgaaggcccg
     1741 gagggccgcg gtggcctccg cggggggcgg cgtcatcttg cccttcagcg gcgccgcgaa
     1801 ggccatgtag ttggcggtgc ggtagtcgtt cttggccatg tcgagcagca ggtagtcgcc
     1861 cccgttgccc gcggtgtgca cgtgggtgtt gatgaagccg ggcgagacca gcttgccccg
     1921 cgccgagatg cgcgcgtcaa ccgcgcccgt gtacggcccg cccgcgtgca cgacgcggtg
     1981 gtcctcgaag accacgctcc cctgctcgtg cacctcgtgg ccgcgcccgt tccacgccac
     2041 cacccagccg ccctcgatca ccgtccgcat gtcggccctc ccagggccag catgctagcc
     2101 cgcccgcctc cgactccgca atcgggaggt gcgccgtgca gtcaaccggc gaggctccac
     2161 tcagggcgtg gagaagggtc cagctgcgag gcggggcccg aagggcgcgc gcgaggcgta
     2221 gtccctctac gttgagcggg cgctcgaggg ccccaacgaa gcagctgggc ccttatccgg
     2281 gcccatcaac gggcgcccag ggcgtggaga agggcccagc tgcgaggcgg ggcccgaaga
     2341 gcgcacgcga ggcgtagtct ctctacgttg agcgggcgct cgagggcccc aacgaagcag
     2401 ctgggccctt atccgcgcct gtcaacgggc gcccagggcg tggagaaggg cccagctgcg
     2461 aggcggggcc cgaagggcgc acgcgaggcg tagtccctct acgttgagcg tgcgctcgag
     2521 ggccccaacg aagcagatgg gcccttatcc gcgccctggg ctagggccgg acgaccttgc
     2581 tgtgcaggat gccgatcgac tcgacctccg cctcgatccg gtcgcccgcc ttgaggaagt
     2641 tgccgctggc tgcgccgacc ccctcgggag tcccggtcgc gatgatgtcg ccgggcagca
     2701 gcgtcatgcc ctgggagagc acctcgatgc actgggccac cgggaagatc atcttggagg
     2761 tgtggctcga ctgccgcaat tgcccgttca ccctcaagga gatcgcgagg gcctgcggat
     2821 cgggcagctc gtcggcggtg accagcaccg gccccatcgg gcagaaggtg tcgagcgact
     2881 tgcccttgaa ccactgctga tgcttcttct gcagatcgcg tgcggtcacg tcgttgatga
     2941 tcgtgtaacc gaacacgtgc ttgagggcgt cggcccgcgc gatgtcgcgc ccgccggtgc
     3001 cgatcaccac cgtcagctcg acctcgtagt cgagctcctt ggtgatcgca tggtgcacca
     3061 cgtcgtcgcc cggcccgacc accgcggtgg ccggcttggt gaagtagacc gggtgttccg
     3121 gcgccgccgc gccgcgctcc gcggcgtgat cggcgtagtt gcggccgagg cagaagatgt
     3181 tccggctcgg ggacgggatc ggggcgagca gccgaacccg cttcagcggg taggccagcc
     3241 gccgctgggc cagctcctcc gccgcggtct ggccgacgag ccgctggccg tgctccaccg
     3301 cctcccgcgc gagggcgcgg accgcgtcgc tgccctgcac gatctcgagg atcgtgcggg
     3361 gaaagccgcc gcgtccccgg cgcaccgtcc cccgctgcac ggccaggtcc ttcgcgagac
     3421 cgcccaggtc gaggacgtgc tcctgccaga gcgcgcccgg cttgggctct ccccgtcctc
     3481 gccggaaggt gacgaggcgc acggcctacc cgctcaccgc caccgcgccc gcgcgcgggg
     3541 gcagggtctt gccggcggcg cgcgcgaagt cctcgagctc gcgcggcagg ccgaagcgaa
     3601 cgtcctcctc gaccacgtgg acctcctgca cgctggagcc gtgcttgcgg cgcagctcct
     3661 cgacgacctc ggtcaccagg tgctcggggg tggaggcgcc ggcggtcacc ccgacccggc
     3721 tcaggccctc gagccactcg ggccggatgt cgttcttgtc gttgatcagg taggacggcg
     3781 tccccatcac ggtggagacc tcgaccagcc ggttggcgtt cgagctgttg gccgcgccga
     3841 tgaccagcag cacgtccacc gcccccgcca ccgtcttcac cgcggcctgg cggttctggg
     3901 tcgcgtagca gatgtcgtcc ttggcgggcc cgacgatctt ggggaagcgc cgccgcagcg
     3961 cctcgatgca gtcgcgggtg tcgtccaccg agagcgtggt ctgggtgagg taggccacct
     4021 tgtcgggatc ggcgaccgcg agccggtcca cgtcctcggg atgcgcgatc aggaacatcc
     4081 ggtccggggc ctcgcccatc gtcccgacga cctcgtcgtg atcggcgtgg ccgaccagga
     4141 tgatcgagta cccctcccgc gcgtagcgca ccgcctcgag atgcaccttg gtgacgagcg
     4201 ggcaggtcgc gtcgatcacg cgcagccctc gccgctgggc ctccgcgcgc accgcgggcg
     4261 agatgccgtg ggcgctgaag atcacggtcg cgtcgtccgg cacctcgtcc agctcgtcca
     4321 cgaagtgggc gcccttggcc cgcagggcct ccaccacgtg gcggttgtgc acgatctcct
     4381 tgcgcacgta caccgggggc gggcagacct gcagggcgag ctcgacgatg tcgatcgcgc
     4441 ggtccacccc ggcgcagaag ccgcggggcc cggccagcac gatctgctgc agcggaagcg
     4501 tggtcatgat gcccgtttcc tcacttgaag tagtacgaga cgcccaccac ccccgagtcc
     4561 gagttgaagc cccggttggg gttggaggtg tgccc
//
LOCUS       DATMCV010000773         2680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_240351, whole genome shotgun sequence.
ACCESSION   DATMCV010000773 DATMCV010000000
VERSION     DATMCV010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240351"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VNU03_17695"
     CDS             <1..1108
                     /locus_tag="VNU03_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HWP76358.1"
                     /translation="TMVLLMGIGYGVSTLGLGSLMAAPAVFAFGLVAFGFLGMGPVTI
                     AVDSYGPVTDNAQSVFELSVIETLPGIKGQLKKDYGFDVNFEEAKHLLEENDGAGNTF
                     KATAKPVLIGTAVVGATTMIFSIIVLLTAGLTQNLDKLSLLHAPFLLGLISGGAVIYW
                     FTGASIQAVTTGAYRAVEFIKANIRLEGSTKASIADSKRVVEICTQYAQKGMFNIFLA
                     VFFATLAFAFAEPYFFVGYLISIALFGLYQAIFMANAGGAWDNAKKVVEVDLKEKGTP
                     LHAATVVGDTVGDPFKDTSSVAMNPIIKFTTLFGLLAVELAVSLTAERGAGFTTALAV
                     VFFVISVIFVWRSFYSMRIKVEGARATAPAMAKH"
     gene            1124..1378
                     /locus_tag="VNU03_17700"
     CDS             1124..1378
                     /locus_tag="VNU03_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76359.1"
                     /translation="MDRSSVLGSLVALLVGLALGAAALSWWEGGRQSTEIESLKVRLA
                     DERGVVQAKVKQLTDELSAEKQRREALEQVVEQQKSGKRR"
     gene            complement(1387..1644)
                     /locus_tag="VNU03_17705"
     CDS             complement(1387..1644)
                     /locus_tag="VNU03_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76360.1"
                     /translation="MRRALLVALSLALLLARDGDAQPELQCPDLPFYLLVGFNYPCPI
                     APRVCRVDYGQCRLGIGIRPGTPCQCWASSNGLWYPGVCIR"
     gene            1869..>2680
                     /locus_tag="VNU03_17710"
     CDS             1869..>2680
                     /locus_tag="VNU03_17710"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HWP76361.1"
                     /translation="MPVVIMSGVGLYALARQQRIQAERIGLELARALATAVDAELRSS
                     ISVLQSLATTVEIDPKNLTAFRQEVQPVLETQPTWAALFLADPSGHRLVDTRFPLDAT
                     LPTVFETASFQRVVRTRAATVGGLARGADGVMLFAVRAPVLRAGRVEYVLTALVKPDG
                     ILNVITRQRVPRDWIISIFDANGLRVARSRSHAETLGGRASESLQTLMATSADEGVGQ
                     TTDLEGGRNYASFSRLGDYGWTVAPGIAAGFVEGGTYRSLAIYGSGILLSVVL"
BASE COUNT          402 a          958 c          896 g          424 t
ORIGIN      
        1 caccatggtg ctgctcatgg gtatcggcta cggggtgagc acgctgggcc tcggcagcct
       61 gatggccgcg ccggcggtgt tcgccttcgg cctcgtggcc ttcggcttcc tcggcatggg
      121 cccggtgacc atcgcggtcg actcctacgg tccggtgacc gacaacgccc aatccgtgtt
      181 cgagctgtcg gtcatcgaga cactgcccgg catcaagggg cagctcaaga aggactacgg
      241 cttcgacgtc aacttcgagg aggccaagca cctgctggag gagaacgacg gcgcgggcaa
      301 caccttcaag gccaccgcca agccggtgct catcgggacc gcggtggtgg gcgcgaccac
      361 catgatcttc tcgatcatcg tgctgctcac cgccggcctc acccagaacc tcgacaagct
      421 ctcgctgctg cacgctccct tcctgctggg cctcatcagc ggcggcgcgg tcatctactg
      481 gttcaccggc gcctccatcc aggcggtcac caccggcgcc taccgcgcgg tggagttcat
      541 caaggccaac atccggctcg agggctccac caaggcctcg atcgcggaca gcaagcgggt
      601 ggtggagatc tgcacccagt acgcccagaa gggcatgttc aacatcttcc tcgccgtctt
      661 cttcgcgact ctggcttttg cgttcgcgga gccctacttc ttcgtgggct acctgatctc
      721 gatcgcgctg ttcggcctct accaggccat cttcatggcc aacgcgggcg gcgcctggga
      781 caacgccaag aaggtggtcg aggtggacct gaaggagaag ggcaccccgc tccacgcggc
      841 caccgtcgtc ggcgacacgg tgggcgaccc gttcaaggac acctcgtcgg tggccatgaa
      901 cccgatcatc aagttcacca cgctcttcgg cctgctcgcg gtggagctgg cggtgtcgct
      961 caccgccgag cgcggggccg ggttcaccac cgcgctcgcg gtggtgttct tcgtcatctc
     1021 ggtcatcttc gtatggcggt ccttttactc gatgcgcatc aaggtcgagg gcgcgcgggc
     1081 gacggccccc gccatggcga agcactgaga ggagggcggc tcaatggacc gctcgagcgt
     1141 gctcggttcc ctggtggcac tcctggtcgg cctggccctc ggcgcggccg ccctctcctg
     1201 gtgggagggc ggccggcaga gcaccgagat cgagagcctc aaggtccgtc tggccgacga
     1261 gcgcggggtg gtccaggcca aggtcaagca gttgaccgac gagctcagcg ccgagaagca
     1321 gcggcgcgag gcgctcgagc aggtcgtgga gcaacagaag agcggcaagc gacgctagcg
     1381 ccgccgctag cggatgcaga cccccggata ccacagcccg ttgctcgacg cccagcactg
     1441 acagggcgtg ccgggccgga tcccgatccc gagccggcac tgcccgtagt cgacccggca
     1501 cacgcggggg gcgatcgggc aggggtagtt gaagcccacg agcaggtaga acggcaggtc
     1561 ggggcactgc agctccggct gggcgtcacc gtcccgggcg agcagcaacg cgagcgacag
     1621 cgccaccagc agggctctcc gcacggccgg tctccctccg cggccaccgt accatcggaa
     1681 ggcgaggccc gcgagtgatc tgatcgccta ctccgcgcca cttcatcgcg tccttcgggc
     1741 ggaaggggcg cgagttgccc catccccgtg ttgcaccatc tggggacctg agccagaatg
     1801 ccgcgatggc cgaagcccct cgcgtcccct tgcgcagacg gcttctcctt ctggcggcgg
     1861 ccggcctcgt gccggtggtc atcatgtccg gagtcgggct ctacgcgctg gcccggcagc
     1921 agcggatcca ggccgagcgc atcggcctcg agctcgcgcg cgccctggcc accgcggtcg
     1981 acgccgagct gcgcagctcc atctccgtgt tgcagtcgct ggccaccacc gtcgagatcg
     2041 accccaagaa cctcaccgcc ttccggcagg aggtccagcc ggtgctcgag acgcagccga
     2101 cctgggccgc gctcttcctc gccgacccgt ccggccatcg tctcgtcgac acccgcttcc
     2161 cgctcgacgc gaccttgccc acggtcttcg agaccgcgag cttccagcgc gtcgtgcgca
     2221 cccgcgcggc cacggtgggc gggctggcgc gcggagccga cggcgtcatg ctgttcgcgg
     2281 tgcgggcgcc cgtcctgcgc gccggccgcg tagagtacgt gctgaccgcg ctggtcaagc
     2341 ccgacggcat cctgaacgtg atcacccgcc agcgggtgcc ccgcgactgg atcatctcga
     2401 tcttcgacgc gaacggcctg cgggtcgccc gctcgcggtc ccacgcggag accctcggcg
     2461 ggcgggcatc ggagagcctg cagaccctga tggcgacctc ggccgacgag ggcgtcgggc
     2521 agacgaccga cctcgagggc ggccgcaact acgcctcgtt cagccggctc ggtgactacg
     2581 gctggacggt ggctcctggc atcgcggccg gcttcgtcga agggggcacc taccgctcgc
     2641 tcgccatcta cgggagcgga atcctcctct cggtggtgct
//
LOCUS       DATMCV010000774         3362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_107860, whole genome shotgun sequence.
ACCESSION   DATMCV010000774 DATMCV010000000
VERSION     DATMCV010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107860"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VNU03_17715"
     CDS             <1..923
                     /locus_tag="VNU03_17715"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP76362.1"
                     /translation="HGPSLARGHYLLPIPAQVQAELEAALAELRRAPVPTLLLLPEHF
                     ALTATARFMDEARRRLDEGPGFVMLDRLPVDAMSRDEAIALYWVLMSLLEPPVAQEWK
                     GTVIYDVRHDGREYSEDTRGALTPVGLEMHTDSSMGEAPPSYVSLFCLRPARSGGMSI
                     VSSAAAAHNHFLTEYPALLRRLYDVFYRDHQEYQAADAAATNFRPVFAWDSRLRTRFN
                     ARHIVRGYEKTGRALDSEGAQAVRLMDEFLGDPAHRLDLWLERGQIQVLNNRVIVHGR
                     TPYEDQEEPELRRHLVRLWLRAGDRRQFRG"
     gene            complement(968..1627)
                     /locus_tag="VNU03_17720"
     CDS             complement(968..1627)
                     /locus_tag="VNU03_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76363.1"
                     /translation="MSRSRRAGVVLVIAAAALAVTPIDAFAQRMGGGARSGSHGAGSP
                     GGFHRGSNHGGPGGRSFAHRRFGGGLIVFAPPFWYGSDLSYDAGPAYPSAPAYGPAVY
                     PGVGGPGVGGTLALAAPAPPTPSVIQYPTGRWELRGDGLTIPYRWVWIPNPPSAPPSP
                     IPPSGEPVTPAPPVSTRPEPPGNTKIYRWTDGQGVLHLTDRLDTVPERYRAQARASRP
                     S"
     gene            complement(1749..>3362)
                     /locus_tag="VNU03_17725"
     CDS             complement(1749..>3362)
                     /locus_tag="VNU03_17725"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWP76364.1"
                     /translation="PTRERTRPDGVTGYLDTLIDLAATSQAERPALLAEAFAAAQIPR
                     GGETARAITNMAARLDVADPAVRAAAREFQEATRARDRIRQTLAVETFKEQESRNLPQ
                     EETTKAELRAAEEKIASLEGRLQAELPRYAQITAARPVPVSELAPLLRPGEAVVQLLP
                     TSDGTYVLLVRDGQAYAHRAAITAGELTRLVRGIRASLEVTDGKLKPFDTAAAARLYT
                     LLLAPLAEPLRGATHLVAVPSGPLLSLPLGLLVTRAPAVAGDYAQTGWLARDVAISVI
                     PSVSSLKSLRQLAGRSAATRPFIGFGDPAFAGAPGATRGLDALGELCRQGEPVDTALV
                     RELPRLRETARELEQIATALGADRASVITGAAATEPAVRTANLADYRVLAFATHGLLP
                     GELKCKSEPALALTPPAIATAADDGLLDASEVAQLKLDADWVLLSACNTASPDGKLGG
                     ESLSGLARAFFYAGARSVLASHWAVASRATVALTTGAFDALAKDASLGRAEALRRSAL
                     KLAGEKDTGHPFFWAPFVLVGDGGAPSRP"
BASE COUNT          428 a         1261 c         1251 g          422 t
ORIGIN      
        1 ggcacggccc ctccctcgcg cgcggccact acctgctgcc gatcccggcg caggtccagg
       61 ccgagctgga ggcggcgctg gccgagctgc ggcgcgcccc ggtgcccacg ctcctgctcc
      121 tgcccgagca cttcgcgctc accgcgaccg cgcgcttcat ggacgaggcg cgccgccggc
      181 tcgacgaggg gccgggcttc gtgatgctgg accggctccc ggtggacgcg atgagccgcg
      241 acgaggcgat cgcgctgtac tgggtcctga tgagcctcct cgagcccccg gtggcccagg
      301 agtggaaggg caccgtgatc tacgacgtgc gccacgacgg ccgggagtac tccgaggaca
      361 cccggggcgc gctgacgccg gtcgggctcg agatgcacac cgacagcagc atgggggagg
      421 cgccgcccag ctacgtgagc ctcttctgcc tgcgcccggc gcgcagcggc gggatgagca
      481 tcgtgtcgag cgcggcggcg gcccacaatc acttcctgac cgagtacccc gccctgctgc
      541 ggcgtctcta cgacgtgttc taccgggatc accaggagta ccaggcggcg gacgcggccg
      601 cgaccaactt ccggcccgtc ttcgcgtggg acagtcggct gcgcacgcgc ttcaacgccc
      661 gccacatcgt ccggggctac gagaagaccg ggcgcgcgct cgactccgag ggggcccagg
      721 cggtgcgcct catggacgag ttcctgggcg accccgcgca tcggctcgat ctctggctcg
      781 agcgcggcca gatccaggtg ctgaacaacc gcgttatcgt ccacggccgc acgccctacg
      841 aggatcagga ggagcccgag ctgcgccgcc atctcgtgcg gctctggctc cgcgccggcg
      901 accgccgcca gttccgcggc tagcgcacgg gcgccgggcg accaggacgg ccgactaggc
      961 tgggcggcta cgacgggcgg ctcgcccgcg cctgggcccg gtagcgctcc ggaaccgtgt
     1021 cgagccggtc ggtgaggtgg agcaccccct gcccgtcggt ccagcgatag atcttcgtgt
     1081 tgcccggggg ctccggcctg gtcgatacag gcggggcggg cgtgaccggc tcaccgctcg
     1141 gcgggattgg cgacggcggc gcgctcggag ggttcggaat ccacacccag cgatacggaa
     1201 tcgtgaggcc gtcgccacgc agctcccagc ggccggtcgg atactggatc acgctcggcg
     1261 tcggcggcgc cggagccgcg agcgccagcg tcccgcccac tccggggccc cccactccgg
     1321 gatagacggc cgggccgtag gccggggcgg atgggtaggc cgggccggcg tcgtacgaga
     1381 gatcggatcc gtaccagaac ggcggcgcga agacgatgag gccgccgccg aaacgccggt
     1441 gcgcgaagga gcgtcccccc ggcccgccgt ggttggagcc gcgatggaat ccgccggggc
     1501 tgccggcgcc gtggctaccg gatcgcgctc ctccgcccat gcgctgagcg aacgcgtcga
     1561 tcggcgtgac cgccagcgcg gccgccgcga tcaccaggac gaccccggcc ctccggctcc
     1621 tgctcatccg ctccctccac cacccccgcc accacggaag atggccggag tgtgcgcccg
     1681 gctttcggcc gcgtcaacgc gcagcgcgcc accgtcccgg cacgccaccg accctgtggg
     1741 gcgcgctctc acgggcggct gggcgccccg ccgtcgccaa ccaggacgaa cggagcccag
     1801 aagaacgggt gcccggtgtc cttctctccg gccagcttga gggccgagcg gcgcagtgct
     1861 tccgcgcgac cgagcgacgc gtcctttgcc agcgcgtcga aggcaccggt ggtgagggcc
     1921 acggtcgcgc gcgaggccac cgcccagtgc gaggccagca ccgagcgggc gcccgcgtag
     1981 aagaaggcgc gcgcgaggcc cgacaggctc tcgccaccca gcttgccgtc cgggctcgcg
     2041 gtgttgcagg ccgacagcag cacccagtcc gcgtcgagct tgagctgggc cacctcactc
     2101 gcgtccagca gcccatcgtc ggccgcggtc gcgatggcgg gcggcgtcag ggccagggcc
     2161 ggctccgact tgcacttgag ctccccgggc agcagcccgt gggtggcgaa ggcgagcacg
     2221 cggtagtcgg ccaggttggc ggtgcgcacc gcgggctcgg tggcggccgc gccggtgatc
     2281 acgctcgccc ggtccgcgcc gagcgcggtg gcgatctgct cgagctcgcg cgcggtctcg
     2341 cgcagccgcg gcagctcgcg caccagcgcg gtgtcgacgg gctccccctg ccggcacagc
     2401 tcccccagcg cgtcgagccc cctcgtggct cccggcgccc ccgcgaaggc cggatcgccg
     2461 aacccgatga aggggcgcgt ggcggcggag cgccccgcca gctggcgcag cgacttgagc
     2521 gacgagacgg acgggatcac gctgatcgcc acgtcgcggg ccagccagcc ggtctgggcg
     2581 tagtccccgg cgaccgccgg tgcccgggtc accagcaggc cgagcggcag gctcagcagc
     2641 gggccgctcg gcaccgcgac gagatgcgtg gcgccgcgca gcggctcggc gaggggggcc
     2701 agcaggagag tgtagaggcg cgcggcggcc gcggtgtcga acggcttgag cttgccgtcg
     2761 gtgacctcga ggctcgcccg gatcccgcgc accaggcggg tcagctcgcc ggcggtgatt
     2821 gcggcgcgat gcgcgtaggc ctgcccgtcg cgcaccagga gcacgtacgt cccgtcgctg
     2881 gtcggcagga gctgcaccac cgcctcgccg ggccgcagca gcggggccag ctccgagacc
     2941 gggaccgggc gcgccgcggt gatctgcgca tagcgcggca gctcggcctg cagccggccc
     3001 tcgaggctcg cgatcttctc ctcggccgcg cgcagctcgg ccttcgtcgt ctcctcctgc
     3061 ggcaggttgc ggctctcctg ctccttgaag gtctccaccg cgagcgtctg ccggatgcgg
     3121 tcgcgcgcgc gggtcgcctc ctggaactcg cgggccgcgg cccgtactgc tggatccgcg
     3181 acgtcgaggc gcgccgccat gttggtgatc gcccgcgcgg tctcgccccc gcgcgggatc
     3241 tgcgccgccg cgaaagcctc cgcgagcagc gccggccgct cggcctgcga ggtggcggcc
     3301 agatcgatca gggtgtcgag gtagccggtc acgccgtcgg ggcgcgtgcg ctcgcgcgtg
     3361 gg
//
LOCUS       DATMCV010000775         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_378053, whole genome shotgun sequence.
ACCESSION   DATMCV010000775 DATMCV010000000
VERSION     DATMCV010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378053"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VNU03_17730"
     CDS             <1..312
                     /locus_tag="VNU03_17730"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWP76365.1"
                     /translation="HSRYDVLLTPGLGQLPVKLGWIDMMMDDVEEYWRRVFAFSPFTV
                     WFNLTGQPAMMLPLGHSASGLPVAVQLVARFGDEATLFRLGAQLETARPWFARKPALA
                     S"
     gene            complement(609..1640)
                     /locus_tag="VNU03_17735"
     CDS             complement(609..1640)
                     /locus_tag="VNU03_17735"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWP76366.1"
                     /translation="MRIGVFGAGAIGCVVGGLLTKAGHDVTLVDQWPEHIETMKRRGL
                     KLSGTCGDHTIPVKALHIHELQGVSTPFDAIFVAVKSYDTEWAAQMALTYLKPSDGVV
                     VDFQNGINDERVASVVGKDRCLGCVITIGAGMYEPGHAMRTDSGAVGFKIGEHDGRDT
                     PRAQALAKVMNDVAGTKVTTNLWGERWSKLAINCMANPLAGLSGLGSAEVRTAPVPRR
                     IAIHVCAEVIKVGRAAGYEVEPIYGIEAQRFVDAAQGKGLEAVETDMGASVKFLTGGR
                     PSMLQDVMKGRRTEIEYLNGYVSQQGRRLGVPTPVNDAVVAAVRSHGVGTLKPDPKNL
                     DPLLQALPR"
     gene            complement(1637..2212)
                     /locus_tag="VNU03_17740"
     CDS             complement(1637..2212)
                     /locus_tag="VNU03_17740"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWP76367.1"
                     /translation="MPTRCAWANGGPLEIEYHDTEWGVPSRDDRHLFEMLILEGAQAG
                     LSWSTILRKRENYRKAFAGFDPVKVARFDARRRAALMRDPGIVRNRLKIEATVANARQ
                     VLAVQDEHGSLAAYLWQFVEGVPVVNAWTAMGQVPAETAESRAMSKALLQRGFRFVGP
                     TICYAFMQATGMANDHVTTCFRYSKRRSRRA"
     gene            complement(2220..>3264)
                     /locus_tag="VNU03_17745"
     CDS             complement(2220..>3264)
                     /locus_tag="VNU03_17745"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP76368.1"
                     /translation="TALRSGLKEVWEFGALVGRHPTMQEVYKAIGRVAASDVSVLLRG
                     ESGTGKEVVARALHHYSRRAGRPFVGISAAAIPGTLLESELFGHEKGAFTDAKERRLG
                     KLELAHGGSVFFDEIGDMPPELQVKLLRALQERSFERVGGHELIRMDVRVMAATHRDL
                     EAMMKSGRFREDLFYRLNVVTLSLPALRDRRGDIPLLGEHFLAKYAESLGERVVTADA
                     MDRLMGYDWPGNVRELENVIQRAMVMASTGAILPEHLPIGPVSAAAAVGTDASLEEII
                     ERKMHECVRGLRGHASANLHGLMVGLVEKPLLRAVMRETKGNQVRAAQLLGINRNTLR
                     KKLKEHGIDPDAV"
BASE COUNT          426 a         1187 c         1123 g          528 t
ORIGIN      
        1 cactcccgct acgacgtgct gctgaccccg ggcctcggcc agctcccggt caagctcggc
       61 tggatcgaca tgatgatgga cgacgtcgag gagtactggc gccgcgtctt cgcgttctcg
      121 ccgttcacgg tgtggttcaa cctgaccggc cagcccgcca tgatgctgcc gctcggtcac
      181 tccgcgagcg ggttgccggt cgcggtccag ctcgtcgcgc gcttcggcga cgaggccacg
      241 ctgttccgcc tcggcgcgca gctggaaacc gcgcgcccct ggttcgcccg caagcccgcg
      301 ctcgcgtctt agggcttcgt cgtcgtcgcg gaactcccag acgttgcgcg cgctcgggct
      361 cctcgtgagc gggtccgggg cgaggcggcc gggtcctgtc ggcgggggcg gctcccggct
      421 ggccgtctcc gtcacccgct cgccggattc gccgctcttc gagcggcgcg ccggactcgg
      481 ggggcccact ccgcggccac ccgggagccg cccccgcggc cacccggccg gtctcgcgcg
      541 ctcccgctca cgaggagccc ggcctgtgcg cgtgcgggag agtcaggcta gtgcggcggg
      601 ctttgaggct agcgcggcag agcctggagg agcgggtcta gattctttgg gtcgggcttg
      661 agggtgccga cgccgtggct gcggacggcg gcgaccaccg cgtcgttcac cggtgtcggg
      721 acgccgaggc ggcggccctg ctggctcacg tagccgttca ggtactcgat ctcggtgcgg
      781 cggcccttca tcacgtcctg gagcatggac gggcggccgc cggtgaggaa cttgacgctc
      841 gcgcccatgt cggtctccac cgcttccagc cccttgccct gggcggcgtc gacgaagcgc
      901 tgggcctcga tgccgtagat cggctcgacc tcgtagccgg cggcgcggcc gaccttgatc
      961 acctcggcgc acacgtggat ggcgatgcgc cggggcaccg gcgcggtgcg cacctcggcc
     1021 gagcccaggc cgctcaggcc cgcgagcgga ttggccatgc agttgatcgc gagcttcgac
     1081 catcgctcgc cccacaggtt ggtggtcacc ttggtgccgg ccacgtcgtt catcaccttc
     1141 gcgagcgcct gggcccgcgg ggtgtcgcgg ccgtcgtgct cgccgatctt gaagccgacc
     1201 gcgccggagt cggtccgcat cgcgtggccg ggctcgtaca tcccggcgcc gatggtgatc
     1261 acgcagccga ggcagcggtc cttgcccacc acggaagcca cccgctcgtc gttgatcccg
     1321 ttctggaagt cgaccaccac cccgtccgac ggcttgagat aggtcagcgc catctgggcc
     1381 gcccactcgg tgtcgtagga cttgaccgcg acgaagatcg cgtcgaacgg cgtcgacacg
     1441 ccctgcagct cgtggatgtg gagcgccttc accgggatgg tgtggtcgcc gcaggtgccg
     1501 gagagcttga gaccgcggcg cttcatggtc tcgatgtgct cgggccactg gtccaccagc
     1561 gtcacgtcgt gcccggcctt ggtgagcagc ccgcccacca cgcagccgat cgctcccgct
     1621 ccgaagaccc cgatcctcat gcccgtcgac tcctccgttt ggaataccga aagcacgtgg
     1681 tgacatggtc gttggccatg ccggtggctt gcatgaacgc gtagcagatc gtcggcccca
     1741 cgaagcggaa gccgcgctgg agcaacgcct tgctcatcgc gcgcgactcg gcggtctcgg
     1801 ccggcacctg ccccatggcg gtccacgcgt tgacgaccgg cacgccctcg acgaactgcc
     1861 acagataggc cgcgaggctg ccgtgctcgt cctggaccgc caggacctgc cgcgcgttgg
     1921 ccaccgtcgc ctcgatcttc agccggttcc gcacgatgcc cggatcccgc atgagggcgg
     1981 ctcgcctccg cgcgtcgaag cgggcgacct tgaccggatc gaatcccgcg aaggccttcc
     2041 ggtagttctc ccgcttgcgc agaatggtgg accaggagag cccggcctgg gctccctcga
     2101 ggatcagcat ctcgaagagg tgccggtcgt cacgcgaggg aacgccccac tcggtgtcgt
     2161 ggtactcgat ctccagcggg cctccgttgg cccaggcgca gcgggtcggc acgggtctcc
     2221 tagacggcgt cggggtcgat gccgtgctcc ttgagcttct tgcgcagggt gttccggttg
     2281 atgccgagga gctgggccgc ccgcacctga ttgcccttgg tctcccgcat gaccgcgcgc
     2341 aggagcggct tctccaccag tcccaccatc agcccgtgga ggttggcgga ggcgtgtccg
     2401 cgcaggccgc gcacgcactc gtgcatcttg cgctcgatga tctcctcgag cgacgcgtcg
     2461 gtgcccaccg cggccgccgc cgagaccggg ccgatcggca ggtgctcggg caggatcgcc
     2521 cccgtcgagg ccatgaccat ggcccgctgg atgacgttct cgagctcgcg cacgttgccg
     2581 ggccagtcgt agcccatcag ccggtccatc gcgtcggcgg tgaccacccg ctcgccgagc
     2641 gactccgcgt acttggccag gaagtgctcc ccgagcagcg ggatgtcgcc gcggcgatcg
     2701 cgcagggcgg gcagcgagag ggtgaccacg ttcaagcggt agaacaggtc ttcccggaag
     2761 cgcccgctct tcatcatggc ctcgagatcg cggtgggtcg ccgccatcac gcgcacgtcc
     2821 atccggatca gctcgtggcc gccgacgcgc tcgaagctgc gctcctggag cgcgcgcagc
     2881 agcttcacct ggagctcggg cggcatgtcg ccgatctcgt cgaagaagac cgaccctccg
     2941 tgggccagct cgagcttgcc caggcgccgc tccttggcgt cggtgaacgc gcccttctcg
     3001 tggccgaaca gctccgactc cagcagggtg cccgggatcg cggcggccga gatgcccacg
     3061 aagggacgcc ccgcgcgccg gctgtagtgg tgcagcgccc gggccaccac ctccttgccg
     3121 gtgccggact cgccgcgcag cagcaccgac acgtcgctgg ccgccacccg gccgatggcc
     3181 ttgtagacct cctgcatggt cgggtgccgg ccgaccagcg cgccgaactc ccagacctcc
     3241 ttgaggcccg agcggagcgc ggtg
//
LOCUS       DATMCV010000776         5654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_176240, whole genome shotgun sequence.
ACCESSION   DATMCV010000776 DATMCV010000000
VERSION     DATMCV010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176240"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="VNU03_17750"
     CDS             complement(<1..1827)
                     /locus_tag="VNU03_17750"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWP76369.1"
                     /translation="MAAESIFFRDLAYVFMAAVAGGALARLAGQPLVLGYVLGGILIS
                     PLTPGPAVSDLHTFELFAEIGVILLMFSVGIEFSLRDLLRVKWVALLGGPLGIALSVA
                     MGLGVASLLGWTPLQGAIVGIVLSVASTMVLARLLMDRGELHSRHGRVMIGITLVEDL
                     AVVVLIVVIPTLGTLEPGRLVTIAGTLGQALLILVPAGYVAAKLIPPLMTRVARMHSD
                     EMFLLVALAVGLGTAALTQAAGLSLALGAFLAGLIINGSEYAHETLARLLPLRDVFVA
                     LFFVTIGALIDPGSLVANVPLLATMVAMVVVGKLVLWTLVVWLFRQSLWTALLVGVGL
                     TQIGEFSFILVQVARTAGHVGPDIYNATLAASLITILINAALVRAAPGWIARLRGVEQ
                     SRPPGGVERAEAEHVVLCGFGRVGSAVGEALETFGIRFVVIERDPDIVRGLRSRAVPC
                     LFGDAAQARLLDEAGAGQAQLVVVALPEMDRAQLAVRAVRALNPRVPVLARAHSASGR
                     DALAEAGATEVIEPELEAAATLIRHALRQLAQPQARVLDYLTRFRGAMNPVPADDRGG
                     SGAAEALPEVHEVRLGAGALADLSLGEARVRERFGVTVVSVER"
     gene            1901..2326
                     /locus_tag="VNU03_17755"
     CDS             1901..2326
                     /locus_tag="VNU03_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76370.1"
                     /translation="MTERRRAPAPRAQAKIKQLETRLAQVEADLARVRTTAARGGLTG
                     VKLAGIEKAMARQVSRAQAGLKDSVNRLSRRLLSARSRREAAQQVAQARASVKESLDR
                     LARTLGESQQKVSREVSLLTRGLKAGVKAGRAAYQGRRR"
     gene            2376..3767
                     /locus_tag="VNU03_17760"
     CDS             2376..3767
                     /locus_tag="VNU03_17760"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HWP76371.1"
                     /translation="MRPVLVCPDDLLEHMVAAAAFPGERPLYLVSRASLRARLGRHGD
                     RAVAGRVTDPAFLHEALKGGRGPVVVAAPSGQLGRVAAAVKEAAPDAPLLVLRDDDRP
                     MSGATVVPLSAFGERIIQPAVDRAAQRVRADRLRAHFYDAERVLILMQDDPDPDAIAS
                     ALALKTLLGRTRTSAPMCTFGTITRPENVAMCKILEIEVEAISAEDIAEFDRVAMVDV
                     QPSFLEERFERVDLVIDHHPMEQPIDARIKDVRPSYGATSTILVEYLRAADIKITQRL
                     ATALLYGIKSDTLGLERGGTKADLEAFSYLYLLANHNALRRIERPELSHEALDVLAEG
                     LAKRRVVQGVFFSHLGRVETMDLIPQFADFGLQAEGVQWSVVSGVVGDEVHVSVRNVG
                     YVKSAGDVTRAAFGDLGVGGGHRTMAKAIFPVQKLGRGASEPGGGGVCQERIIDRFLR
                     AIGAGRPPEGPPS"
     gene            3764..4072
                     /locus_tag="VNU03_17765"
     CDS             3764..4072
                     /locus_tag="VNU03_17765"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HWP76372.1"
                     /translation="MIVIYLLMALLGSAATIFALQNRDPVEIWFLAWGVKGMPLALVI
                     LLSLLVGVVFTSLSGLVKVLKMRYRIRQLETQVGQLIAAQPVPPPPPRLPQSPAPPPA
                     "
     gene            4083..5237
                     /gene="mgtE"
                     /locus_tag="VNU03_17770"
                     /pseudo
     CDS             4083..5237
                     /gene="mgtE"
                     /locus_tag="VNU03_17770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
     gene            5266..5430
                     /locus_tag="VNU03_17775"
     CDS             5266..5430
                     /locus_tag="VNU03_17775"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWP76373.1"
                     /translation="MIVAAVVGVVIPLFLKSFRVDPAIASSVIITTFTDVFGFFSFLG
                     LATLLTRFLL"
BASE COUNT          861 a         2018 c         2014 g          761 t
ORIGIN      
        1 ccgctccacc gagaccaccg tgaccccgaa gcgctcgcgc acccgcgcct ccccgaggga
       61 cagatcggcg agcgcacccg cgccgagcct cacttcgtgc acctcgggca gcgcctccgc
      121 ggcacccgac ccgccgcggt cgtcggccgg gaccgggttc atcgctccgc ggaaccgggt
      181 gaggtagtcg agcacgcgcg cctggggctg ggcgagctgg cgcagcgcgt ggcggatcag
      241 ggtggccgcc gcctccagct cgggctcgat gacctcggtg gcgcccgcct ccgcgagggc
      301 gtcgcggccc gaggcgctgt gggcccgcgc caggaccggc acgcgcgggt tgagcgcccg
      361 gaccgcgcgc accgcgagct gggcccgatc catctcgggc agcgccacca ccaccagctg
      421 ggcctgaccc gcgcccgcct cgtcgagcag acgggcctgc gcggcgtccc cgaagagaca
      481 cggcaccgcg cgggagcgca gcccccgcac gatgtccgga tcgcgctcga tcaccacgaa
      541 gcggatcccg aaggtctcga gcgcctcgcc caccgcgctg ccgacgcggc cgaagccgca
      601 gagcaccacg tgctccgcct ccgcgcgctc gaccccaccg ggggggcggc tctgctcgac
      661 cccgcggagg cgggcgatcc agccgggggc cgcgcgcacg agcgccgcgt tgatcaggat
      721 ggtgatcagc gaggcggcga gggtggcgtt gtagatgtcg gggcccacgt ggccggcggt
      781 ccgcgcgacc tggaccagga tgaaggagaa ctcgccgatc tgggtgaggc cgaccccgac
      841 cagcagcgcg gtccagagcg attgacggaa gagccagacc accagggtcc acagcacgag
      901 cttgcccacc accaccatcg ccaccatggt ggcgagcagc gggacgttgg cgaccaggct
      961 gcccggatcg atcagcgcgc cgatggtcac gaagaagagc gccacgaaga cgtcgcgcag
     1021 cggcaggagc cgggccagcg tctcgtgggc gtactccgag ccgttgatga tgaggccggc
     1081 caggaacgcg cccagcgcga gggagaggcc ggcggcctgg gtgagggccg cggtgccgag
     1141 tcccaccgcg agggccacga gcaggaacat ctcgtcgctg tgcatgcggg ccacccgcgt
     1201 catgagcggc gggatgagct tggccgccac gtagccggcc gggaccagga tgagcagcgc
     1261 ctggccgagg gtgcccgcga tcgtgaccag gcgccccggc tcgagcgtgc ccaacgtggg
     1321 gatgaccacg atgaggacca ccaccgcgag gtcctcgacg agcgtgatgc cgatcatcac
     1381 ccggccgtgg cgcgagtgca gctcgcctcg atccatgagc aggcgcgcga gcaccatggt
     1441 gctcgccacc gagaggacga tgccgacgat ggcgccctgg agcggcgtcc agcccagcag
     1501 gctcgccacc ccgagcccca tcgcgaccga gagcgcaatc ccgagcgggc cgcccaggag
     1561 cgccacccac ttcacgcgca gcaggtcgcg cagcgagaac tcgatgccga ccgagaacat
     1621 gagcaggatc accccgatct cggcgaacag ctcgaaggtg tgcagatccg agaccgcggg
     1681 cccgggggtg agcgggctga tcaggatgcc ccccagcacg tagcccagca ccaggggctg
     1741 gcccgcgagc cgggcgagcg cgccgccggc caccgcggcc atgaacacgt acgccaggtc
     1801 ccggaagaag atggattcgg cggccacgcc caagtctcgc atccaccccc cggtggggcc
     1861 aagcctcgag cgtcgtatac tgcagggcgg aggagacgcc atgaccgagc gacgcagagc
     1921 gcccgcgccc agggcgcagg cgaagatcaa gcagctcgag acgcgcctgg cccaggtcga
     1981 ggccgacctc gcgcgcgtgc gcaccaccgc ggcccgcggc gggctcacgg gtgtcaagct
     2041 ggccggcatc gagaaggcga tggcccggca ggtgtcgcgc gcccaggcgg ggctgaagga
     2101 ctcggtgaat cgcctcagcc ggcggctcct cagcgcgagg tcgcggcgcg aggcggcaca
     2161 gcaggtcgcc caggcgcgcg cgagcgtgaa ggagtcgctc gaccgtctgg cccgcaccct
     2221 gggcgagtcc cagcagaagg tgagccggga ggtgtccctc ctcacccgtg gcctcaaggc
     2281 cggcgtgaag gccggccgcg cggcgtacca gggccggcgc cgctagccgg attttcgctt
     2341 ggcccccccg cggggtgggc cgagaatggg gccatatgcg accggtcctg gtttgtccgg
     2401 atgatctcct cgagcacatg gtggccgccg ccgcgttccc gggcgagcgg ccgctctacc
     2461 tggtctcgcg ggcgagcctg cgcgcgcggc tcggccggca cggcgatcgg gcggtggcgg
     2521 gccgggtcac cgatccggcc ttcctgcacg aggcgctcaa gggcggccgc gggccggtgg
     2581 tggtggccgc gccgtccggg cagctcggcc gcgtggccgc ggcggtgaag gaggccgcgc
     2641 cggacgcccc gctgctggtg ctgcgcgacg acgaccggcc gatgtcgggc gcgaccgtgg
     2701 tgccgctctc cgccttcggc gagcgcatca tccagcccgc ggtggaccgc gcggcccagc
     2761 gggtccgcgc cgaccgcctg cgggcccact tctacgacgc ggagcgggtc ctgatcctca
     2821 tgcaggacga tcccgatccc gacgcgatcg cctccgcgct cgcgctcaag accctgctcg
     2881 gccgcacccg cacctcggcc ccgatgtgca ccttcggcac catcacccgc cccgagaacg
     2941 tggcgatgtg caagatcctg gagatcgagg tcgaggcgat cagcgcggag gacatcgcgg
     3001 agttcgaccg ggtggcgatg gtggacgtgc agccctcgtt cctcgaggag cgcttcgagc
     3061 gggtggacct ggtgatcgac caccatccga tggagcagcc gatcgacgcg cgcatcaagg
     3121 acgtgcgccc gtcctacggg gcgacctcca cgatcctggt ggagtacctg cgcgcggccg
     3181 acatcaagat cacccagcgg ctcgccaccg cgcttctcta cggcatcaag tccgacaccc
     3241 tcggcctcga gcgcggcggc accaaggcag acctggaagc gttctcctat ctctatctcc
     3301 tcgccaacca caacgcgctc cgccgaatcg agcggcccga gctctcgcac gaggcgctcg
     3361 acgtcctcgc cgagggtctc gccaagcggc gcgtggtgca gggcgtgttc ttctcgcacc
     3421 tcggccgggt cgagacgatg gacctcatcc cccagttcgc cgacttcggc ctgcaggccg
     3481 agggcgtgca gtggtcggtc gtatcggggg tggtgggtga cgaggtgcac gtctcggtcc
     3541 gcaacgtcgg ctacgtgaag agcgcgggcg acgtcacccg cgccgccttc ggcgatctgg
     3601 gcgtgggcgg aggccatcgc acgatggcca aggcgatctt cccggtgcag aagctcgggc
     3661 gcggcgcgag cgagccgggg ggcggtggag tctgccagga gcgcatcatc gaccgcttcc
     3721 tgcgcgcgat cggagcgggc aggccgccgg agggccctcc gtcatgatcg tcatctacct
     3781 gctgatggcg ctgctcggga gcgcggccac catcttcgcg ttgcagaacc gcgacccggt
     3841 cgagatctgg ttcctggcct ggggcgtgaa ggggatgccg ctggcgctcg tgatcttgtt
     3901 gtcgctgctg gtcggggtcg tcttcacctc gctgagcggc ctggtgaaag tcctcaagat
     3961 gcggtaccgg atccgccagc tggagactca ggtgggccag ctcatcgcgg cccagcccgt
     4021 tcccccgccg ccgccccgct tgccgcagag ccccgcaccg ccccccgcct agccccgtcg
     4081 tcatgggcga ggcaacccgg ctcaccgaca ccgtccggga tctgctggag tcgggacgcc
     4141 gcgagcgcct caaccagatc ctcgaggacg cccaccccgc ggacatcgcg tcggctctgc
     4201 gcgagctccc cctccacgac caggtgacgc tcttccgcct gctcggccgc gaatcggctg
     4261 gggcggtgct ggccgagctg ggagaccagg cggtgcacga gctggcgggg gccctcgacg
     4321 agggcgagat ctcgagcatc ctcgaccgga tgcggcccga cgacgccgcc caggtcctcg
     4381 aggagctgcc cgccgagcag gccgagaagg tgctggacct catgagggag gagaagtccg
     4441 aggaggtcca ggggctcctc gagtacggcg agaagaccgc cggccggctc atgaccccgg
     4501 acatgctcgc gatcctcgag tacgtcacgg tggctcaggc gatcgagcag gtccggaagt
     4561 caccggtggc cgagaccgcc ccggtcctct acgtggtgga cgatcacgac catctggtgg
     4621 gcagcctgcc ctggcggcgg ctgatcacgg ccgacccgac ggctccggtg ggactcctgc
     4681 gccaggagga gccggtgagt gtgaccccgg agaccgacca ggaggaggtc gcccggctcg
     4741 tcgccaagta caacctggtg gccatcccgg tggtcgacag cggccatcgc ctgctggggg
     4801 tgatcacggt cgacgacatc atcgacgtca tccgggagga ggccaccgag gacatccagc
     4861 gcctcggtgg ggcggccggc gacgagaccg tcctggaccc ggcccgggtg gtcttcccga
     4921 agcgcctggt ctggctgctg atcaacctgg cgaccgccat cctggccgcc tcggtgatcg
     4981 gcctcttcga gaactcgatc cgggagctgg ccctcctggc cgtgttcatg ccgatcgtgg
     5041 cctccatggg cggcatcggg accacccaaa ccgccaccgt ggtggtgcgg gggctcgccc
     5101 tgggggacat gtcggcggcc catctctggc gggtgctgcg gaaggagacc accctggccg
     5161 tgaccatcgg gctggccaat ggcctggtca tggccgccat cgcctacctg tggaaggggc
     5221 aggccctcct cgccgggatc atcgcgctgg cccatgacgc tcaacatgat cgtggccgcg
     5281 gtggtcgggg tggtcattcc cctcttcctc aagagcttcc gggtggaccc cgccatcgcc
     5341 tcgagcgtga tcatcacgac cttcaccgac gtcttcgggt tcttctcctt cctcggtctg
     5401 gccaccctcc tcacccgatt cctgctctag tccgggcggc aaaggtaaga tttacccttg
     5461 gagcgtgtaa aaacgtgtag cgattttcat gcccaccggg atgcaaaagg ttccgaggtc
     5521 gcagaccctg agaaaatcgc gggttcctga cggtcttcgg gcgcgcgcac atgacgcggg
     5581 ctggcatgag ggttgcctca acagcagctc ggaggaagcc ccggaatctc gggctcctcc
     5641 ggctctcgga acag
//
LOCUS       DATMCV010000777         6922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_977587, whole genome shotgun sequence.
ACCESSION   DATMCV010000777 DATMCV010000000
VERSION     DATMCV010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977587"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..814
                     /locus_tag="VNU03_17780"
     CDS             311..814
                     /locus_tag="VNU03_17780"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWP76374.1"
                     /translation="MTDRPERSRRGWRTLFARLTEGQRRDVKEILAENYAAEIWQARE
                     LAGHAERLSRYPDRRERLLAIAAREEEHARWLREAIERLGGRPPEPATSPADARTNWE
                     RLIGDLEAEKEAAERMLRDAALVERDHPDVARLLERISREEAAHQEELVWLLARSTDR
                     VVLDQAP"
     gene            811..1521
                     /locus_tag="VNU03_17785"
     CDS             811..1521
                     /locus_tag="VNU03_17785"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWP76375.1"
                     /translation="MIPLRDSVTLLSRPVVTHVLIALNVAVFLYELTLGPELEGFVRT
                     WGFIPARYFWLTPLDPEEWVARYLPLLASMFLHGGWAHLIGNMVYLWIFGDNVEDRLG
                     HGRYLVFYVVCGVSASLAQAYLHPDSLVPTVGASGAISGVLGAYLVLFPYARVLTFVP
                     LLLFFPLIEVPAVIYLGLWFLMQLMNGTVALALMGEPGGVAWWAHVGGFVAGAALAPL
                     LRGRPRDPRRWRDQYASW"
     gene            1552..2397
                     /locus_tag="VNU03_17790"
     CDS             1552..2397
                     /locus_tag="VNU03_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWP76376.1"
                     /translation="MGIGDVFWLLFMLSAVQPVLQQKMLEASRLRVLSRFERKRRSRA
                     IALVHRQETMSFLGFPIVRYIDINDSEAILRAIKLTEPDVPIDLIVHTPGGLVLAAGQ
                     IAHALRRHRAKVTVFVPHYAMSGGTLLALAADEIVMDPNAVLGPVDPQLGQQPAASVL
                     TVLEQKKPEHIDDQTLILADVSRKAIVQVARTVQDLLSERMSLEQATSLAEKLSTGTW
                     THDYPILSEEGKSLGLPISTEMPDEVYEFMRLFPQPVRARPAVEYVPMRYGSRQGGSL
                     SPRQL"
     gene            2394..2894
                     /locus_tag="VNU03_17795"
     CDS             2394..2894
                     /locus_tag="VNU03_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HWP76377.1"
                     /translation="MTAHGRSRGALGPGLERFARRVTEWTGSSLAFAGATGFVVLWAI
                     TGPLFHFSDTWQLVINTFTSIVTFLMVVLIQRSQNKDSLAVHLKLNEIVAAIDGASNR
                     LINVEDLTEEEVRTLREYYRKLVEIARREESLTRSHSIEEAAARARDKRRRSQALNAT
                     ASRSGA"
     gene            2911..3213
                     /locus_tag="VNU03_17800"
     CDS             2911..3213
                     /locus_tag="VNU03_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76378.1"
                     /translation="MDGDLAGVVDELTRRGFSEDFRVVDGRLQVVGTRERLRPEDCAI
                     VEYHRFEGISDPDDMAIVYGIETVSGTRGTVVDAFGVYSDPALSAALRDVSIRGPA"
     gene            3230..3649
                     /locus_tag="VNU03_17805"
     CDS             3230..3649
                     /locus_tag="VNU03_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76379.1"
                     /translation="MALLLHSNLDVLIPPRWVTVESRVNDTLARYPSTAPVFVQFGRL
                     YVDRPGEVYPAFPGLTVGEYARHNGVALEPLLVELNAAAESEEAARLWSRSPVREPGS
                     GGGFSPTLGYTASHRAAEDAAPNWVPVVQVQATRGPE"
     gene            3831..4151
                     /locus_tag="VNU03_17810"
     CDS             3831..4151
                     /locus_tag="VNU03_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76380.1"
                     /translation="MRKAALVGLVAAIGALMLAASPAAAWHRWHGGVFIDVGPWWIPP
                     PRYYVAPPPAVIVEPPPVYVQQAPTQPAQESYWYYCASKQGYYPYVPSCPEPWVPVPA
                     RPPQ"
     gene            4148..4666
                     /locus_tag="VNU03_17815"
     CDS             4148..4666
                     /locus_tag="VNU03_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HWP76381.1"
                     /translation="MSRRLALGIPLALLATACATLPPGPSVMVLPGTLKTFEDFQTDD
                     AVCRQWAALQTGTTRKRAAGESAASGATIGTVLGAALGAAFGAAAGNPGIGAAVGAGT
                     GLLGGTVVGADTAEGAAYEVQHRYDMAYMQCMYSKGNQIPVARGSLPAYTAISPGPAD
                     VPPPPPPGRVGQ"
     gene            5043..5678
                     /locus_tag="VNU03_17820"
     CDS             5043..5678
                     /locus_tag="VNU03_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWP76382.1"
                     /translation="MQRDVIAELKWEPRVRPNEIGVAVKDGVVTLMGWVDSYTKKWAA
                     EEATHRVRGVKAVANDIEVRLPGDAERTDADIAVAAVRAVGWNAFVPSGQVDITVSKG
                     WVTLKGEVEWQFQRDEAERVVRNLMGVKAVTNLITVKPRLTPSGLKEKIEDALLRSAK
                     TDAERITVEVQGNKVILRGTVRALAEREEAERVAWSAPGVASVENRIAISY"
     gene            5886..6317
                     /locus_tag="VNU03_17825"
     CDS             5886..6317
                     /locus_tag="VNU03_17825"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HWP76383.1"
                     /translation="MLGIGLALFGLMVRTLMRLVKELLGPSLDFRVVVAEVLFMLVMV
                     ELVRLLILYLEEHRVAVDFMVELGIVATLREIVLRGVIELTWQQITALSVFFVALGAL
                     LRFADLRTPDSPLLGRGDISRGTDGSHAGAVQADTAPRAGA"
     gene            6444..6809
                     /locus_tag="VNU03_17830"
     CDS             6444..6809
                     /locus_tag="VNU03_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76384.1"
                     /translation="MGPSLDPFVVAVGEHTIADADFEDVISAGCGRRRRDSVAKAAAV
                     DRGEPPAADMITLIRPMLATLVPKPFHRPGWVYEEKYDGIRTLAYRRRRRIRLYSRSL
                     TEAVERGGTLETEQGAGQY"
BASE COUNT         1071 a         2231 c         2526 g         1094 t
ORIGIN      
        1 acggagcaag gctcaagcgc atcgtgatcc ccaccggggc ccgcgccccc gcctcctcgg
       61 gtgtgtcttc acccaacgcc ggcggtcccg cccgacgaaa ccttcggatc agcggcccgg
      121 atcagcggga agatgtcccg cgtccgggct ggcgtcgccc ttgcacgcac gctgcacggc
      181 gaatgccagc cgactggagg agcaggccga tggcaaatct agagggctgg cggcgggcga
      241 tgcgacgctc tccgatgcgg cgattcgacc ggttcttctt cgtggccatc cagagggtcg
      301 gatgagcgcg gtgaccgatc gcccggagag atcgaggcgc gggtggcgca cgctcttcgc
      361 ccggctcacc gaggggcagc gacgcgatgt gaaggagatc ctggccgaga actacgcggc
      421 cgagatctgg caggcccgcg agctcgccgg gcacgcggag cggctgtcgc gctacccgga
      481 ccgccgcgag cggttgctcg cgatcgcggc gcgcgaggag gagcacgcgc gctggctccg
      541 ggaggcgatc gagcggctgg ggggccgccc gcccgagccc gccacgagtc cggccgacgc
      601 gcggacgaac tgggagcgcc tgatcggcga cctggaggcg gagaaggagg cggccgaacg
      661 gatgctgcgc gacgccgccc tggtggagcg cgaccatccg gacgtcgcgc ggctgctcga
      721 gcggatcagc cgggaggagg ccgcgcacca ggaggagctg gtctggctcc tcgcgcgcag
      781 caccgaccgg gtggtcctgg accaggcgcc gtgatcccgc tgcgcgactc cgtgacgttg
      841 ctgagccggc cggtggtcac ccatgtcctg atcgcgctca acgtcgcggt gttcctttac
      901 gagctgacgc tcgggcccga gctggagggc ttcgtgcgca cgtggggctt cattcccgcg
      961 cgctacttct ggctgacgcc gctcgatccg gaggaatggg tggctcgcta cctgcccctt
     1021 ctcgcctcca tgttcctgca cggcggttgg gcccatctga tcggcaacat ggtctacctg
     1081 tggatcttcg gcgacaacgt ggaggaccgc ctcggccacg ggcgctacct cgtcttctac
     1141 gtggtgtgcg gagtctcggc gtccctcgcc caggcttacc ttcatcccga ctcgttggtg
     1201 ccgaccgtcg gggccagcgg ggcgatcagc ggcgtgctcg gcgcgtacct ggtgttgttc
     1261 ccctacgccc gggtgctcac cttcgtcccg ctgttgctct tcttcccact catcgaggtg
     1321 ccggcggtga tctacctggg tctctggttc ctgatgcagc tcatgaacgg aaccgtggca
     1381 ctcgcgctga tgggcgagcc ggggggagtg gcgtggtggg cgcacgtcgg cggattcgtg
     1441 gccggggcgg cgctcgcacc gctcctgcgc gggcggccgc gagatccgcg aaggtggcgg
     1501 gaccagtacg cttcgtggtg aacgaaaggc catccatcca aggaggaacc gatgggtatc
     1561 ggtgacgtgt tctggctgct cttcatgctg tcggccgtgc agccggtgct gcagcagaag
     1621 atgctggagg cgagccggct gcgcgtgctc agccggttcg agcgcaagcg ccggagccgg
     1681 gcgatcgcgc tggtgcaccg gcaggagacg atgagcttcc tcggcttccc gatcgtgcgc
     1741 tacatcgaca tcaacgactc ggaggcgata ctgcgcgcga tcaagctcac cgagccggac
     1801 gtgccgatcg acctcatcgt gcacaccccc ggcgggttgg tgctggccgc cgggcagatc
     1861 gcccacgcgc tgcgccgcca ccgggccaag gtcaccgtgt tcgtgccgca ctacgcgatg
     1921 tcgggcggca ccctgctcgc gctggccgcg gacgagatcg tgatggatcc caacgcggtg
     1981 ctgggcccgg tggacccgca gctgggccag cagccggccg cctcggtcct gaccgtgctc
     2041 gagcagaaga agcccgagca catcgacgac cagaccctga ttctggccga cgtctcccgc
     2101 aaggcgatcg tgcaggtggc ccggacggtg caggatctcc tgtccgagcg tatgtcgctg
     2161 gagcaggcga cttcactggc cgagaagctc tccaccggca cgtggacgca cgactacccg
     2221 atcctctcgg aggaggggaa gtcgctcggc ctgccgatca gcaccgagat gccggacgag
     2281 gtctacgagt tcatgcgcct gttcccgcag cccgtccggg cccggccggc ggtggagtac
     2341 gtgccgatgc gctatgggag tcgccaaggc gggagcctct cgccccgcca gctatgaccg
     2401 cgcacggccg gagccgcggc gccctcgggc ccggactgga gcggttcgcc cgccgggtca
     2461 ccgagtggac cggcagctcg ttggcgttcg ccggcgcgac cgggttcgtc gtgctgtggg
     2521 cgatcacggg gcccttgttt cacttctcgg acacctggca gctcgtgatc aacaccttca
     2581 cgagcatcgt gacattcctg atggtggtcc tgatccagcg ctcccagaac aaggactcgc
     2641 tcgcggtgca cctcaagctg aacgagatcg tggccgcgat cgacggggcc agcaatcggc
     2701 tcatcaacgt cgaggacctg acggaggagg aagtgcggac gctgcgcgag tactaccgga
     2761 agctggtgga gatcgcccgg cgcgaggaga gtctcacgcg atcgcactcg atcgaggagg
     2821 cggcggcccg cgcgcgggac aagcggcgac ggtcccaggc gctcaacgcc acggcctcgc
     2881 gctctggagc ctgaccgagg aggcgccgcc gtggatggcg acctcgcggg cgtggtggac
     2941 gagctcaccc gccggggttt ttccgaggat ttccgcgtgg tggacggccg gctgcaggtg
     3001 gtgggcaccc gcgagcgcct ccggccggag gattgcgcga tcgtcgagta ccaccggttc
     3061 gaggggatct cggatcctga cgatatggcg atcgtgtacg gtatcgagac cgtgagcggg
     3121 acgcggggga cggtggtcga cgccttcggc gtctactcgg accccgcgct gagcgcggcg
     3181 ctgcgggacg tgtcgatccg gggacccgcg tgacgtgcat gcgggtccgg tggcgcttct
     3241 cttgcactcg aacctcgacg tgctcatccc cccacgctgg gtcaccgtcg agtcgcgcgt
     3301 caacgatacg ctggcgcgct atccgagcac cgcgcccgtg ttcgtccagt tcggccgcct
     3361 ctacgtggat cgcccggggg aagtataccc ggcgtttccc gggctcaccg tcggggagta
     3421 cgcgcggcat aacggggtgg cgctcgagcc gctgctggtc gagctgaacg cggcggccga
     3481 gtccgaggag gcggcgcggc tctggtctcg atcgcccgtc cgcgagcccg ggtcaggggg
     3541 tggcttctcg ccgaccttgg gctacaccgc cagtcatcgc gcggcggaag acgcggcgcc
     3601 caactgggtc ccggtggtgc aggtgcaggc gacacgaggg cccgaatagt gccgagcgtg
     3661 aggcacgtgc cgcgcgggtg cgcggccacc cgaggcgcgg gtgcgggcgc acccgggctg
     3721 cctgcgccgc cgtgccgaag cggcgcctcg accccgcgat gggcgctgat gtggccccgg
     3781 acactgcgtg gctacggttt tgcagtcctg gtcctccggg ggaggcgacg atgagaaagg
     3841 cggcgctggt gggactggtc gcggcaatag gcgcgctcat gctggcggcc agcccggccg
     3901 cggcctggca tcggtggcac ggaggcgtgt tcatcgatgt cggcccctgg tggatcccac
     3961 cgccgcgata ctacgtcgca ccgccgcccg ccgtcatcgt ggagccgccg ccggtgtacg
     4021 tgcagcaggc gcccacccag cccgcgcaag agtcctactg gtactactgc gcgagcaagc
     4081 aaggctacta cccgtacgtg ccgagctgtc ccgaaccgtg ggtgcccgtg cccgcgaggc
     4141 cgccccagtg agccgccggc tggccctcgg gatcccgctc gcgctcctgg cgaccgcgtg
     4201 cgcgacactg ccgcccggcc cgagcgtgat ggtgctcccg gggacgctca agacctttga
     4261 ggacttccag accgacgacg cggtctgccg gcagtgggcc gcgctgcaga ccgggaccac
     4321 ccgcaagcgg gcggcgggcg agagcgcggc gagcggcgcg acgatcggca ccgtgctcgg
     4381 cgcggcgctg ggcgccgcgt ttggcgccgc cgccggcaat ccgggaatcg gggccgcggt
     4441 gggggcgggg accgggctcc tcggcggcac cgtagttggc gcggacaccg ccgagggggc
     4501 cgcgtacgag gtgcagcatc gctacgacat ggcctacatg cagtgcatgt attccaaggg
     4561 caaccagatc ccggtggccc ggggatcgct gccggcgtac accgcgatct cgcccgggcc
     4621 ggcggatgta ccgccgccac ccccgcccgg acgggtcggc cagtgagcgc gatgggatat
     4681 cgcggcgtat ctggagcgcg cgaagcgggc cccgctccag tgccggatcg tgtcctgggc
     4741 gccttggtcg caccgctccg ggtcttcacc gttgcggagg catcgatgaa tgtggggcgc
     4801 acacggtcca gaaccccgac tggtacgcgg cccggacttt gcgagcggca gccctcgtcg
     4861 gctagcgcag tcgctcattt gcgagacacg acatgctggg agcctccacg gtgagcccgt
     4921 taaggcgggc gacgcactga ctgcggccgc acgcgaattg aacgcggtga tgctcgcccg
     4981 cgtgaaaagg gaggaacagt catggcaacg gcaacagaaa cgcgacgcgc acggatgagc
     5041 taattcagcg ggacgtgata gcggagttga aatgggagcc ccgcgtgcga ccgaacgaga
     5101 tcggggtggc ggtcaaagat ggggtcgtga cgctgatggg ctgggtcgat tcctatacca
     5161 agaaatgggc tgcggaggag gccacgcatc gggtgcgcgg cgtgaaggcc gtggccaacg
     5221 atatcgaggt ccggctcccc ggcgatgccg agcgcaccga cgccgacatc gcagtggcgg
     5281 cggttcgggc ggtcgggtgg aatgccttcg tgccgagcgg gcaggtcgac atcacggtgt
     5341 cgaagggatg ggtcacgcta aagggcgagg tggagtggca attccagagg gacgaggccg
     5401 agcgggtcgt ccggaacttg atgggtgtca aggcggtcac caacctgatc acggtgaagc
     5461 cgcgactgac gccctccggg ttgaaggaga aaatcgaaga cgcgctcctg cggagtgcga
     5521 agacggacgc cgagcggatc acggtagagg tgcaggggaa caaggtgatt ctgaggggaa
     5581 cggtgcgggc gttggcagag cgagaagaag cggagcgagt ggcgtggtcg gctccgggcg
     5641 tcgcctcggt ggaaaaccgg atcgcgatct cgtactaggt ccgcgaatcg catgtgctgc
     5701 tgcgggtgac aagagcaccc gggaacatcc gctacgaaat gagggtccac ggcggagcgg
     5761 ccggctgacg gccggccgcc gccagcgttc cggtcgatat tcagggggtc atgaaaatcc
     5821 gggcttctca tacggacgtc caccgagtgg cccgacgctt cttggagcca gcccaggaca
     5881 tcctggtgct cggcatcggg ctggcgctgt tcgggttgat ggtgcgtacc ctcatgagac
     5941 tcgtcaaaga actcctcgga cccagcctgg acttccgcgt ggttgttgct gaggtgctat
     6001 tcatgctggt gatggtcgag ctcgtgcgtt tgctcatcct ctaccttgag gagcaccggg
     6061 tggcggtcga cttcatggtg gagctcggaa tcgtggcgac ccttcgcgag atcgtcctgc
     6121 gtggggtgat cgagttgacc tggcagcaga ttacggcgct gagcgtcttc ttcgtcgccc
     6181 tcggtgcgct gctccgtttc gccgatctgc gcacgccaga ttcgcctctc ctgggacggg
     6241 gtgacatctc aagaggtacc gacggaagcc acgctggcgc agtacaggcg gacacggcgc
     6301 ctcgagcagg cgcttgagcc ccagcgacac ggcgacattg acggttaggc cgtcgcttcg
     6361 ttatgcagaa acaccgtgct cgacacctgc gctaggactt ccggctcgag gtcaggggca
     6421 gcctgcagtc atggtcggtt ccgatgggcc cgtcgctgga tcccttcgtg gtagcggtgg
     6481 gagagcacac catcgcggat gcggactttg aggacgtcat atcggccgga tgcgggagac
     6541 gacgtcggga ctccgtcgcc aaggcggcgg ccgtcgatcg aggggagcct ccggcggcgg
     6601 acatgatcac actgattcgc ccgatgctgg cgacgctcgt gccgaaaccg ttccaccggc
     6661 cgggctgggt ctacgaggag aagtacgacg gcatccgcac ccttgcctac cggcgtcggc
     6721 gacgaattcg gctgtattcg cgcagtctca ctgaagcagt cgaacggggc gggacacttg
     6781 agacggagca aggcgcaggt cagtattgaa ccccatcaaa ttacgagttg cgacgcgcgg
     6841 aggcgtccgc gcctgacggc tggcgtcatt cgtctgccgc ggcaagtggt aggctgccca
     6901 cgccgacgac ctgacggaga gc
//
LOCUS       DATMCV010000778         6735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_705844, whole genome shotgun sequence.
ACCESSION   DATMCV010000778 DATMCV010000000
VERSION     DATMCV010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705844"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VNU03_17835"
     CDS             <1..252
                     /locus_tag="VNU03_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWP76385.1"
                     /translation="LIDISALEIVDSFIGRALSNIAKMARVLDADTVVVGMRPAVAIT
                     LVELGLNLDGVRTALDAEKGMALIRRRFDERGRLDARGS"
     gene            236..640
                     /locus_tag="VNU03_17840"
     CDS             236..640
                     /locus_tag="VNU03_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HWP76386.1"
                     /translation="MPAVREESYDIHSDEDVVRVRQLTRRWAVDLGFTLVDQTKIVTA
                     ASELARNTLIHGGGGVTRLEALQEGERRGLRLTFEDKGPGMADVPQALRDGFTTGSGL
                     GLGLGGARRLCNEFDITSVPGQGTRVRITRWK"
     gene            637..1635
                     /locus_tag="VNU03_17845"
     CDS             637..1635
                     /locus_tag="VNU03_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013321371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWP76387.1"
                     /translation="MIVTRAPLPFPITEPSQVGEARRQILATALAIGLDADQRGKLAL
                     VVNELGTNLVKHGSGGELIARVPEGPPAVELLALDRGAGMGSVAECFRDGYSTAGSQG
                     TGLGAIGRLAARVDVYSTQPGGTAIVALVGADAPLGRLETGAVSVPRAGEEVCGDAWA
                     VAAAQECPALMVVDGLGHGLGAADAARLAVRAFEGSAELPPSALVATIHDALRGSRGA
                     AVSVARIDWARGTVTYAGLGNVAGVVLSGGDARHLVSGNGTAGHDAGRISEFSYPWAE
                     DAVLVMHSDGLGSRWQMDRYPGLPRRHPTLIAGVLYRDWTRGRDDVTVVAARLRRA"
     gene            1632..3797
                     /locus_tag="VNU03_17850"
     CDS             1632..3797
                     /locus_tag="VNU03_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP76388.1"
                     /translation="MSLPLISLFVRHEQDVVAARQRARQIAARLGFESQDQTRIATAV
                     SEIARNAFMYGGGGKIEITVEGRYAPQLLVMRVSDRGPGIRDLDAVLSGRYRSSTGMG
                     LGMLGARRLVDHFEVKSRPGAGATVTLGKLFPRRAPEIGPAGLAALADALAREVPQGA
                     LEEVRLQNQELLRTLDELRERQEDLSRLNAELQDTNRGVLALYAELDEKADTLRRADE
                     MKSRFLSNMSHEFRTPLNSMLALSRLLLEDAERPLTAEQKRQVGYIRQAAEDLTELVN
                     DLLDLAKVEAGKIAIRPAEFDVHDLFAALRGMLRPLLLNDQVNLVFEEPEGVPPMHTD
                     EGKVSQILRNLLSNALKFTERGEIRVSARSVGEGPGGPLIAFSVADTGIGIAPADQQR
                     IFEEFGQLENPVQRRVRGTGLGLPLVRRLATLLGGSVSLDSTPGEGSTFTTVLPARFA
                     TEREPAETAAAAEPASLEPGQVPVLVVEDSAQDLLLYEHYFRGSRFHPVIARTLPDAR
                     RLIDQIHPVALVLDIRLAGEDAWAFIGEVRRRDDTRGLPLVVVSSIDDQAKGLTLGAD
                     AYAIKPVHPAWLLGTLSRLLDRGPERRVLVIDDDEIARYLVRNHLAGAPFVVTETADP
                     VQGLRDARADRPDFIVLDLVMPEMSGFDVLARLKEDPVTMDIPVVVLTSKTLTDEERR
                     RLAPHARRIVSKQALAEGREIEHLRDILSDAEPREAGRG"
     gene            3790..5718
                     /locus_tag="VNU03_17855"
     CDS             3790..5718
                     /locus_tag="VNU03_17855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP76389.1"
                     /translation="MAESRGLILNVDDYEASRYARSQMLQRSGFRVLEAGTGAEALRL
                     ASDVGPDLVLLDVNLPDMDGFEVCRRLRAQPLTLTVPIVHISATFLSDRAEQLAYEGG
                     ADSYLTDPVEPAVLLATIHSLLRLRRAEEGLRAASREWRATFDAIGDGICLLAPDGTV
                     ARCNGAFATLLDRAPADLVGRPWTEIWRRLGGGEEPSPTAQVERTRRRETFDLHREQG
                     DLHREQGWFRLLVDPVLEGESVTGLVCIVTDVTIERQAADVRAALFAREQEAREEAEA
                     SNRAKDEFLAMLGHELRNPLDVIGTAVRVLDSPTGEPDRDAGTTRRARGVLARQVLQL
                     GRLVDDLLDVGRVTTGKIRLVKVPVNLADTVQRSVAGLAPEGGTPAHRITVRAEPIWV
                     EADQARVEQIVMNLLSNAVKYTAPGGDIEISVTGDGRTARLAVRDTGVGMTPYMLERV
                     FDLFFQGERTLERAEGGLGIGLTLVRRLAEMHGGRVEAESPGQGRGTTVTVELPQIAA
                     PAEAPGAPPPARPSPRRILIVEDSRDSRDMLRYLLERAGHEVHEAADGPSGVEAILKV
                     RPDIALVDVGLPGLDGYEVARRVRADAAGGKVRLVALTGYGLPDDHRRSQEAGFDAHL
                     VKPVDPARLAAVIEAPAR"
     gene            complement(5715..6056)
                     /locus_tag="VNU03_17860"
     CDS             complement(5715..6056)
                     /locus_tag="VNU03_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76390.1"
                     /translation="MSEQTQDVRARLDRLEQGVTRLAEGPERASLVETVQALRETVER
                     LEGVERDERHRLGHDLRVPLNAIAGWTHILKLDASSAATVTRAVDVFDRNVRALTRLI
                     ESYTSEDPSAS"
     gene            complement(6077..6538)
                     /locus_tag="VNU03_17865"
     CDS             complement(6077..6538)
                     /locus_tag="VNU03_17865"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP76391.1"
                     /translation="MPPLRQRVEDIPLLVTGFIEEFNAKYDKRITGVDDATLKILMSH
                     AWPGNVRELRNVVERAFIACEGDVITAKILPGTSPVAPVASWSGDSDILTAPIGLPLR
                     EVEKQFVLRTLAAESNNKTRAADRLGISTKTLHNMLQRWGLLHRQIPRGST"
BASE COUNT          905 a         2432 c         2486 g          912 t
ORIGIN      
        1 ctgatcgaca tctccgcgct cgagatcgtg gactcgttca tcggccgcgc cctcagcaac
       61 atcgccaaga tggcccgcgt cctcgacgcg gacacggtgg tggtgggcat gcggcccgcg
      121 gtggccatca ccctcgtcga gctcggcctc aacctggacg gcgtacgcac cgccctcgac
      181 gcggagaagg gcatggccct gatccgccgg cggttcgacg agcgcgggag gctcgatgcc
      241 cgcggttcgt gaggagagct acgacatcca ctcggacgag gacgtggtgc gcgtccggca
      301 gctgacccgc cggtgggcgg tggacctcgg cttcaccctc gtcgaccaga ccaagatcgt
      361 caccgcggcc agcgagctgg cccgcaacac cctcatccac ggcggaggcg gggtcacccg
      421 gctcgaggct ctgcaagaag gcgagcgacg cgggctaagg ctcaccttcg aggacaaggg
      481 ccccggcatg gccgacgtgc cgcaggccct gcgcgacggc ttcaccaccg gctccggcct
      541 cgggctgggt ctgggcggcg cccggcgtct ctgcaacgag ttcgacatca cctcggtgcc
      601 cggccagggc acccgcgtgc gcatcacgag gtggaagtga tcgtcacgcg cgccccgctg
      661 ccgttcccga tcaccgagcc gagccaggtg ggcgaggcgc ggcggcagat cctggccacc
      721 gccctggcga tcgggctcga cgcggaccag cggggcaagc tcgccctcgt ggtgaacgag
      781 ctcggcacca atctggtcaa gcacggcagc ggaggtgagc tgatcgcccg ggtgccggag
      841 ggtcctcccg cggttgagct gctcgccctg gaccggggcg ccgggatggg gagcgtggcg
      901 gagtgcttcc gcgacggcta ctcgaccgcg ggcagccagg ggaccgggct cggcgcgatc
      961 gggcgcctgg ccgcccgcgt cgacgtgtac tcgacccagc cggggggcac cgccatcgtg
     1021 gccctggtgg gcgccgacgc gccgctcggc cgcctcgaga ccggcgcggt gagcgtgccc
     1081 cgggcgggcg aggaggtttg cggcgacgcc tgggcggtgg ccgccgcgca ggaatgcccc
     1141 gccctgatgg tggtggacgg cctggggcac gggctcggcg ccgccgacgc ggcgcggctc
     1201 gcggtgcggg ccttcgaggg gtccgcggag ctgccgcctt cggccctggt cgcgaccatc
     1261 cacgacgcgc tccgcggcag ccgcggcgcc gcggtatcgg tggcgcgcat cgactgggca
     1321 cgcgggacgg tgacctacgc gggactcggc aacgtggcgg gggtggtgct gagcggcggc
     1381 gatgcgcggc acctcgtctc cggcaacggc accgccggcc acgacgcggg ccggatcagc
     1441 gagttctcct atccctgggc cgaggacgcg gtgctcgtca tgcactccga cgggctcggc
     1501 agccgatggc agatggaccg atatccgggt ctcccccggc gccaccccac gctcatcgcc
     1561 ggcgtgctgt accgggactg gacccgaggt cgcgacgacg tgacggtcgt ggcggcccgg
     1621 ctgcggcggg catgagcctc ccgctcatct ccctcttcgt ccgccacgag caggacgtgg
     1681 tggcggcgcg ccagcgggcc cggcagatcg cggcgcgcct cggcttcgag agccaggatc
     1741 agacccgcat cgccaccgcg gtgtccgaga tcgcccgcaa cgcgttcatg tacgggggcg
     1801 gcggcaagat cgagatcacg gtggagggcc gctacgcgcc gcagctcctg gtgatgcggg
     1861 tgagcgatcg cgggcccggc atccgcgacc tcgacgcggt cctctccggg cgctaccgct
     1921 cctccaccgg catgggcctg ggcatgttgg gcgcccggcg cctcgtcgac cacttcgagg
     1981 tcaagagccg cccgggcgcg ggcgcgacgg tgaccctcgg caagctcttc ccacgccgcg
     2041 cgcccgagat cgggccggcc ggactcgcgg cactcgcgga cgccctcgcg cgcgaggtgc
     2101 cgcagggggc gctggaggag gtccggctgc agaaccagga gctcctccgc accctcgacg
     2161 agctgcgcga gcgccaggaa gacctctcgc ggctcaacgc ggagctgcag gacaccaacc
     2221 gcggcgtcct cgcgctctac gcggagctgg acgagaaggc ggacaccctg cgtcgcgccg
     2281 acgagatgaa gtcgcgcttc ctctccaaca tgagccacga gttccgcacc ccgctgaact
     2341 cgatgctggc cctctcgcgc ctcctgctgg aggacgcgga gcggccgctg accgccgagc
     2401 agaagcgcca ggtgggctac atccgccagg ccgcggagga tctcaccgag ctggtgaacg
     2461 acctgctcga cctcgccaag gtcgaggcgg gcaagatcgc gatccgtccc gccgagttcg
     2521 acgtgcacga cctgttcgcg gccctgcgcg gcatgctgcg gcccctgctg ctcaacgacc
     2581 aggtcaacct ggtcttcgag gagccggagg gcgtgccccc gatgcacacc gacgagggca
     2641 aggtctccca gatcctccgc aacctgctct ccaacgcgct caagttcacc gagcgcggcg
     2701 agatccgggt cagcgcgcgg tccgtcggcg agggcccggg cggtcccctg atcgccttct
     2761 cggtcgcgga caccggcatc ggcatcgccc ccgcggatca gcagcgcatc ttcgaggagt
     2821 tcggccagct cgagaatccg gtccagcgcc gggtgcgcgg caccgggctc ggcctgcccc
     2881 tggtgcgtcg gctggccacg ctcctgggcg gctcggtgtc gctcgacagc accccgggcg
     2941 agggctcgac cttcaccacc gtgctgccgg cgcgattcgc gaccgagcgg gagcccgcgg
     3001 agaccgcggc cgccgcggaa cccgcctcgc tcgagccggg gcaggtgccg gtgctggtgg
     3061 tggaggacag cgcccaggac ctgctgctct acgagcacta cttccgcggc tcccggttcc
     3121 atccggtgat cgcgcgcacc ctgcccgacg cgcgccggct catcgaccag atccacccgg
     3181 tcgcgctggt cctcgacatc cgcctcgcgg gcgaggacgc ctgggccttc atcggcgagg
     3241 tgcggcgacg ggacgacacg cgcgggctgc cgctggtcgt ggtctcgagc atcgacgacc
     3301 aggccaaggg actgacgctg ggcgccgacg cctacgcgat caagccggtg catcccgcct
     3361 ggctgctcgg caccctgagc cgcctgctcg accgcggccc cgagcggcgg gtgctggtca
     3421 tcgacgacga cgagatcgcc cgctatctcg tacgcaacca tctcgcgggc gcgccgttcg
     3481 tggtgaccga gaccgccgac ccggtgcagg ggctgcgcga cgcccgcgcg gaccggcccg
     3541 acttcatcgt gctcgacctg gtgatgcccg agatgtccgg cttcgacgta ctcgcccgtc
     3601 tcaaggagga cccggtcacg atggacatcc cggtcgtggt cctgacctcg aagaccctca
     3661 ccgacgagga gcgccgccgg ctcgccccgc acgcccgacg gatcgtctcc aagcaggcgc
     3721 tcgccgaggg ccgcgagatc gaacatctgc gcgacatcct ctccgatgcg gagccgaggg
     3781 aggccgggcg tggctgagag ccgggggctc atcctgaacg tcgacgacta cgaggcgtcg
     3841 cgctacgccc gcagccagat gctgcagcgg tcgggtttcc gggtgctgga ggccggcacc
     3901 ggcgcggagg cgctccgtct cgcgagcgac gtgggaccgg acctcgtgct cctcgacgtc
     3961 aacttgccgg acatggacgg cttcgaggtg tgccgccgac tgcgggctca gccgctcacg
     4021 ctgacggtac ccatcgtgca catctcggcc acgttcctga gcgaccgggc ggagcagctg
     4081 gcctacgagg gtggcgcgga cagctatctc accgatccgg tcgagccggc ggtgctgctc
     4141 gccaccatcc actcgctgct gcggctgcgc cgcgcggagg aggggctgcg ggcggccagc
     4201 cgcgagtggc gggccacctt cgacgccatc ggcgacggca tctgtctgct cgccccggac
     4261 ggcacggtgg cccgctgcaa cggggccttc gcgaccctgc tcgaccgtgc gcccgccgat
     4321 ctggtcgggc ggccgtggac ggagatctgg cgccggctcg gcggcggcga ggagccgtcg
     4381 ccgaccgcgc aggtggagcg cacccggcgc cgcgagacct ttgacctgca ccgcgagcag
     4441 ggcgacctgc accgcgagca gggctggttc cgcctgctgg tggatccggt gctggagggc
     4501 gagtcggtga ccggcctcgt gtgcatcgtg accgacgtga cgatcgagcg gcaggcggcc
     4561 gacgtgcgcg ccgcgctctt cgcgcgcgag caggaggcgc gggaggaggc ggaggcgagc
     4621 aaccgggcca aggacgagtt cctcgccatg ctgggccacg agctgcgcaa ccccctcgac
     4681 gtcatcggga ccgcggtgcg ggtgctcgac tcgccgaccg gcgagcccga ccgggacgcg
     4741 gggaccaccc ggcgggcgcg cggggtcctc gcgcggcagg ttctccagct cgggcggctc
     4801 gtcgacgacc tcctcgacgt cggccgcgtc accaccggca agatccggct ggtgaaggtg
     4861 ccggtgaacc tggccgacac ggtgcagcgc tcggtcgcgg gcttggcgcc cgagggagga
     4921 accccggcgc accggatcac ggtgcgggcc gagccgatct gggtcgaggc cgaccaggcg
     4981 cgcgtcgagc agatcgtgat gaacctcctg tccaacgcgg tgaagtacac cgcccccggc
     5041 ggcgacatcg agatctcggt gaccggcgac gggcgcaccg cgcgcctcgc ggtgcgggac
     5101 accggcgtcg ggatgacgcc ctacatgctc gagcgcgtct tcgacctctt cttccagggc
     5161 gagcggaccc tcgagcgggc cgaggggggg ctcggcatcg ggctcaccct ggtgcgccgg
     5221 ctcgccgaga tgcacggcgg gcgcgtggag gcggagagtc ccgggcaggg ccgcgggacc
     5281 acggtcacgg tggagctgcc ccagatcgcg gcgcccgcgg aggcgcccgg cgcaccgccg
     5341 cccgcccggc cgagcccgag gcgcatcctg atcgtcgagg acagtcgcga cagccgcgac
     5401 atgctccgct acctgctcga gcgcgcgggc cacgaggtgc acgaggccgc cgacgggccg
     5461 agcggggtgg aggccatcct gaaggtgcgc ccggacatcg cgctggtgga cgtggggctg
     5521 cccgggctcg acggctacga ggtcgcgcgc cgtgtccggg ccgacgcggc ggggggcaag
     5581 gtccggctgg tggccctcac cggctacggg ctgcccgacg accaccggcg ctcgcaggaa
     5641 gcgggcttcg acgcccacct cgtgaagccg gtggacccgg cgcgcctcgc ggcggtgatc
     5701 gaggcgccgg ctcgctagct agcgctcgga tcctccgagg tgtagctctc gatcagccgg
     5761 gtcagggcgc ggacgttgcg gtcgaagacg tccaccgccc gggtcacggt ggccgcgctg
     5821 ctcgcgtcga gcttcaggat gtgggtccag cccgcgatgg cgttgagcgg cacccgcagg
     5881 tcgtgcccca gccggtgacg ctcgtcgcgc tccacgccct ccagccgctc gaccgtctcc
     5941 cgcagggcct gcacggtctc cacgagcgac gcgcgctcgg gcccttcggc cagccgcgtc
     6001 accccctgct cgagccggtc caggcgggcc ctcacgtcct gcgtctgctc gctcacgcgg
     6061 gctctcatct cgccgatcac gtcgaccccc gggggatctg ccggtgcagg agtccccagc
     6121 gctgcagcat gttgtggagc gtcttggtgc tgatgccgag ccggtcggcc gcccgcgtct
     6181 tgttgttgct ctccgcggcc agggtgcgca gcacgaactg cttctcgacc tcgcgcaggg
     6241 gcaggccgat cggcgcggtg aggatgtcgg aatcgccgct ccacgacgcc accggcgcca
     6301 ccggggacgt gccgggcagg atcttggcgg tgatcacgtc gccctcacag gcgatgaacg
     6361 cgcgctccac cacgttgcgc agctcgcgga cgttgcccgg ccacgcgtgc gacatgagga
     6421 tcttgagcgt ggcgtcgtcg acgccggtga tgcgcttgtc gtacttggcg ttgaactcct
     6481 cgatgaagcc ggtgacgagg agggggatgt cctcgacccg ctggcggagc gggggcaccg
     6541 cgagggtgaa gacgttgagc cgtagtagag gtcttcgcgg aacgctccct cccgcaccgc
     6601 cgcgaccggg tccttgttgg tggcggcgac gacgcggacg tcgacggtga tctcggtctt
     6661 gccgccgagg cggcggaagg cgccctcctc gaggacgcgg aggaacttgg cctgggtgcc
     6721 gggctgcatc tcggc
//
LOCUS       DATMCV010000779         4101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_640636, whole genome shotgun sequence.
ACCESSION   DATMCV010000779 DATMCV010000000
VERSION     DATMCV010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640636"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VNU03_17870"
     CDS             complement(<1..794)
                     /locus_tag="VNU03_17870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP76392.1"
                     /translation="MRDQKVFYGWWVVGAFSVTTFMSTGVRHAVGPFLKPIVADLNLD
                     RASFSAVIALSLFLYGVFMPLAGMALDRFSVRVVASAGTLLLVASLILTAAVRNVWEF
                     TAVYGVLVPLGLAGTGPVIASGVVARWFSRRRGTALSVLGSASMTGMSLLVPAVTWLV
                     LTTGWRTTYVIIAIMVLAISLPLCIWVLRDSPESVGLLPDGGTARPGALNAPVERVTG
                     TEALQTLAFWQLAGSFFTCGFSMSLLSAHGIPMLTDHGYTPMFASWT"
     gene            complement(832..1671)
                     /locus_tag="VNU03_17875"
     CDS             complement(832..1671)
                     /locus_tag="VNU03_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP76393.1"
                     /translation="MSTRRPVAVVVGATSKWQADGRNTKLAHGKALDDGDVPVGIRWG
                     VGGAVAQKFAKEGLHVVLTTRSAPNAKGLDEAIRAQGGASSIVELDLVSEASITKAFA
                     TIRAQVGEPEVLVYNAGYLEGRDLPPDKELLEHVPLEIFETAQHIASRGPFLVAKEVL
                     PAMRKRGGGSFLISNNSSSLRGRKRLTGQSLYYPRVMMRTLAQVLTEEYSEHGVHVAN
                     VVIDGLIDSPGTRALPRAQKQPEIVMNPVKIAEAFYYLHTQDRSCWTHELQLTPFSTK
                     PSF"
     gene            complement(1697..2500)
                     /locus_tag="VNU03_17880"
     CDS             complement(1697..2500)
                     /locus_tag="VNU03_17880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP76394.1"
                     /translation="MSPDPAPRMAVHRSGRGPDLVLFHGGMGSWKHWIRNVGPLSERF
                     TVHALDHPSYGDSAPVPRETTGPQYLDLVHAVLVEAFPGDTPLRFAGFSFGAAIASNM
                     ARRLGSRVSHLAIVSPGGFPARKFGERPIRSYKEAGGDDRLFREICRHNLLVNMLSDE
                     ASVSEETLDIQVECVRRTRFDSRKVSAGGTLLGDLAEVARRGGKIRLLWGEHDDSAFR
                     PAKLLIGEIQTAVPDLDVHRIAKAGHWSAYENAPEVNRLLLEFFGGGAT"
     gene            complement(2511..3407)
                     /locus_tag="VNU03_17885"
     CDS             complement(2511..3407)
                     /locus_tag="VNU03_17885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP76395.1"
                     /translation="MRFGVWLPNCRHLATPETIRGAAVRAEALGYDSVWVSDHVVVPR
                     ANVANFGTTIFDPLITLAVVAGATSRVRLGTTVLIVPYRQAVVTAKMVSSLDALSGGR
                     VILGVGAGWVAAESAMLGVPFAERGPMTDEYLRAMQELWTSASPSFSGKYVQFHDLHF
                     EPKPVQRPLPIWVGGHGKASLRRAAELGAAWHPINRSPDELRAGVAELVRLSKARGRA
                     TPPALTLRNDVRILRAGESAPASAHAGRVIAGEPAAVADQITELAACGVEELVLELLA
                     ADAPELDAQMAAFAERVRPRVA"
     gene            complement(3415..>4101)
                     /locus_tag="VNU03_17890"
     CDS             complement(3415..>4101)
                     /locus_tag="VNU03_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWP76396.1"
                     /translation="LFPVAQADDYVRGSIQIVDPETGKFETLYDRCEGERLRGPNDLV
                     FDGAGGFWFTDLGKTRERDSDRGVVYYAKADGSLIKEAIFPLERPNGIGLSPDEKTLY
                     VVETPTARCWAFKLSGPGQIESANGPYRGEKGTVIAGLGGYQMFDSLAVDGEGHVCVA
                     TLITGAVSDIWPDGSRVDQYALPDMMVTNVCFGGKDLRTAFATLSMGGTLVSFEWPRP
                     GLPLHYLNRG"
BASE COUNT          634 a         1465 c         1433 g          569 t
ORIGIN      
        1 gtccacgacg cgaacatcgg cgtgtagccg tggtcggtca gcatcgggat gccgtgcgcc
       61 gagagcaggc tcatcgagaa gccgcaggtg aagaacgagc cggcgagctg ccagaacgcc
      121 agggtctgga gcgcttcggt ccccgtcacc cgctcgaccg gcgcgttgag cgcgcccggc
      181 ctcgcggtgc cgccgtcggg caacaggccg accgactccg gcgagtcgcg gagcacccag
      241 atgcagagcg gcagcgagat ggccaggacc atgatcgcga tgatcacgta cgtggtgcgc
      301 cagccggtgg tcagcacgag ccaggtcacc gcgggcacca gcaggctcat cccggtcatc
      361 gacgcgctgc cgagcacgga gagcgcggtg ccgcgccgac ggctgaacca gcgggcgacg
      421 acgccggagg cgatcaccgg cccggtgccg gccagaccca gcggcaccag caccccgtag
      481 accgcggtga actcccagac gttgcgcacc gccgcggtca ggatcagcga ggccaccagg
      541 agcagggtgc cggccgaggc aacaacccgg acactgaacc tatccagggc catgccggcc
      601 agcggcatga acaccccgta gaggaagagg ctcagcgcga tgacggccga gaagctggcg
      661 cggtcgaggt tgagatcggc cacgatcggc ttcaggaagg gcccgaccgc gtggcgcacg
      721 ccggtcgaca tgaacgtggt gaccgagaag gcgcccacca cccaccagcc gtagaagacc
      781 ttctggtctc gcatgccccc tcgactatat cccacgccgc gggcgatctg cctagaagct
      841 aggcttggtc gagaacggcg tcagctggag ctcgtgggtc cagcaggatc ggtcctgagt
      901 gtggaggtag tagaacgcct ccgcgatctt gaccgggttc atgacgatct ccggctgctt
      961 ctgcgcccgc ggcagcgcgc gggtgcccgg ggagtcgatg agcccgtcga tcaccacgtt
     1021 ggcgacgtgc acgccgtgct ccgagtactc ctcggtgagc acctgcgcca gcgtgcgcat
     1081 catcacgcgc gggtagtaga gcgactgccc ggtgagccgc ttgcgccctc ggagagacga
     1141 cgagttgttc gagatcagga acgagccgcc gccgcgcttg cgcatcgcgg gcagcacctc
     1201 cttggcgacc aggaacggcc cgcggctcgc gatgtgctgc gcggtctcga agatctcgag
     1261 cgggacgtgc tccagcagct ccttgtcggg cggcaggtca cgcccttcga ggtagcccgc
     1321 gttgtagacc aggacctccg gctcgccgac ctgcgctcgg atcgtggcga acgccttcgt
     1381 gatggaggcc tccgacacca ggtcgagctc cacgatcgag ctggccccgc cctgggcccg
     1441 gatggcctcg tcgagaccct tcgcgttggg ggcgctccgc gtcgtcagca cgacgtggag
     1501 gccttccttg gcgaacttct gggccaccgc gccgccgacg ccccagcgga tgccgaccgg
     1561 cacgtcgccg tcgtcgagcg ccttgccatg ggccagcttg gtgttgcggc catccgcctg
     1621 ccacttggac gttgctccca ccacgaccgc gaccggtcga cgggtcgaca tgacgaatcc
     1681 tcctatgtcc tgggctctac gttgcgccgc cgccgaagaa ctccagcagc agccggttga
     1741 cctcgggcgc gttctcgtac gccgaccagt ggccggcctt ggcgatccgg tgaacgtcca
     1801 gatccgggac cgcggtctgg atctcgccga tcagcagctt cgcgggccgg aacgccgagt
     1861 cgtcgtgctc gccccacagc aggcggatct tcccgccccg tcgcgcgacc tcggccaggt
     1921 cgccgagcag ggtaccgccg gcgctgacct tgcgactgtc gaagcgggtt ctccgcacgc
     1981 actccacctg gatatcgagc gtctcctcgg acacgctcgc ctcgtcgctc agcatgttca
     2041 ccagcaggtt gtggcggcag atctcgcgga agagtcgatc gtcgccgccc gcctccttgt
     2101 agctgcggat cgggcgctcc ccgaacttgc gcgcgggaaa gccgccgggc gagacgatcg
     2161 ccaggtggct cacgcgcgat ccgagccgcc gcgccatgtt cgacgcgatg gcggcgccga
     2221 aggagaagcc cgcgaagcgg agcggcgtgt cgcccgggaa ggcctcgacg agcaccgcgt
     2281 gcacgaggtc gaggtactgg gggccggtgg tctcgcgcgg caccggcgcg gagtcgccgt
     2341 aggaggggtg gtcgagcgcg tgcacggtga accgctccga cagcgggccc acgttgcgga
     2401 tccagtgctt ccacgagccc atgccgccgt ggaagaggac gaggtccggc ccgcggccgg
     2461 accggtgcac ggccatgcgg ggcgcgggat cggggctcac gccggcgaga tcaggcgacg
     2521 cgagggcgga cgcgctcggc aaacgcggcc atctgggcgt cgagctcagg cgcgtccgcg
     2581 gcgaggagct ccagcaccag ctcctcgacc ccgcacgcgg ccagctctgt gatctggtcc
     2641 gctaccgccg ccggctcgcc cgcgatcacg cggccggcgt gagcggacgc cggcgcgctt
     2701 tcccccgcgc gcaggatgcg cacgtcgttg cgcagggtca gcgcgggcgg ggtcgcgcgg
     2761 ccgcgggcct tcgagagacg cacgagctcg gccaccccgg cgcgcagctc gtcgggcgag
     2821 cggttgatcg ggtgccacgc cgcgcccagc tcggccgcgc ggcgcaggga ggccttgccg
     2881 tgtccgccca cccagatcgg caggggccgc tgcaccggct tcggctcgaa gtgcaggtcg
     2941 tggaactgca cgtacttgcc ggagaacgac ggcgacgcgc tcgtccacag ctcctgcatc
     3001 gcgcgcaggt actcgtcggt catcggcccg cgctccgcga acggcacgcc gagcatggcg
     3061 ctctccgccg ccacccagcc cgcgcccacg cccaggatca cgcggccacc gctcagcgcg
     3121 tcgagcgacg agaccatctt ggcggtcacc accgcctgcc gatagggcac gatcagcacg
     3181 gtggtgccga ggcgcacccg gctcgtcgcc cccgcgacca ccgcgagggt gatcaacgga
     3241 tcgaagatgg tcgtcccgaa gttggccacg ttggcgcgcg gcaccaccac gtggtcgctc
     3301 acccacaccg agtcgtagcc gagcgcctcg gcccgcaccg ccgcgccccg gatcgtctcc
     3361 ggggtggcga ggtgccggca gttgggcagc cagaccccga agcgcatcag cggttcagcc
     3421 ccggttcaga taatgcagcg gcaggcccgg gcgcggccac tcgaacgaca ccagcgtccc
     3481 gcccatcgag agcgtggcga aggcggtgcg cagatccttc ccgccaaagc acacgttggt
     3541 gaccatcatg tcggggagcg cgtactggtc cacccggctg ccgtcgggcc agatatcgct
     3601 caccgcgccg gtgatcaggg tggccacgca cacgtggcct tcaccgtcca ccgcgagcga
     3661 gtcgaacatc tggtagccgc cgaggccggc gatcacggtg cccttctccc cgcggtaagg
     3721 cccgttggcg gactcgatct gcccggggcc cgagagcttg aacgcccagc agcgcgcggt
     3781 gggcgtctcg accacgtaga gggtcttctc gtccggcgag aggccgatgc cgttgggccg
     3841 ctcgagcggg aagatcgctt ccttgatcag cgagccgtcg gccttcgcgt agtagaccac
     3901 cccgcggtcg ctgtcgcgct cgcgcgtctt gccgaggtcg gtgaaccaga agccgcccgc
     3961 cccgtcgaac accaggtcgt tgggcccgcg caggcgctcg ccctcgcagc ggtcgtagag
     4021 ggtctcgaac ttgccggtct ccggatcgac gatctggatg ctgccccgca cgtagtcgtc
     4081 ggcctgggcc accgggaaga g
//
LOCUS       DATMCV010000780         4167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_40174, whole genome shotgun sequence.
ACCESSION   DATMCV010000780 DATMCV010000000
VERSION     DATMCV010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40174"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VNU03_17895"
     CDS             <1..817
                     /locus_tag="VNU03_17895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP76397.1"
                     /translation="TAVSVMRPVAPSEIVTASDRESVRLDEQVLDLVRLDRVLGLKAT
                     PPSMRLPVVVLRGGGKPFGVVVDELLGKEEIVIKSLGALLEGVGPFSGATISGEGRVI
                     LLLDPTRLRDAATTSPTMPTATSREEERADETRSRVLLVDDSVSIRKFVGQMLEKAGF
                     DVLTAVDGQDAIQQLTEQTVDAVITDLEMPRVNGYALIEDLRRRSTTREIPVIVLTTR
                     AGAKHVGLARRLGVRHYVAKPVEEQSFIRLVASVTSVPEAVLAVAGRSGTMAD"
     gene            821..1762
                     /locus_tag="VNU03_17900"
     CDS             821..1762
                     /locus_tag="VNU03_17900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HWP76398.1"
                     /translation="MANAKVLVVDANQDSRAALAHALTEAGYEAAVATSGSFALTMLE
                     WEQPDLIVSYAKVQDMDGYELFTMVRRDPATKDTPFLLLAGRDRPIALAATEAGVDMT
                     VTGDFTLDTVMTRVQELLGDVPASAPPKPRSEPPPAVSKAPAKMPARPPAVTAAPMPA
                     PAAATVPPPPAPQPKPPVEAARSSFQGSLGVMDLTELTQVISLGVKTGELHLSLSKGE
                     GIILFEAGRVVHAEFAERSGEAAFGAMVAASQREGEARFCFNRADREAMAGRPRTISR
                     SVEQLLLSVAQEMDEMSHGAGGGREPAATRATRTGEA"
     gene            1766..2635
                     /locus_tag="VNU03_17905"
     CDS             1766..2635
                     /locus_tag="VNU03_17905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP76399.1"
                     /translation="MPKVLVVDDSLSVRKVVQRALESRRIEVLSAASGTEALEQIGRE
                     APDLIVCDVIMPDMDGYQICDFVKKHPTLGHTPVLLISGIVNGTVLERAAKVRSDDVM
                     RKPFAAEELLQRIESFLNAQSRTPSSTPPPAAVTAPVERPAPAAVTAPVERPIPVPVI
                     DRPAVVLESAAPSVDLKVLLDGVAQLPGVSLTALIDREGALMEAAGGLLPEATLAGAL
                     AACLVDSTVGVGQALAQGRLQSVILEYDAGVVLLNAVGEGAMLAVVLGDSAVLGKVRY
                     HVKKALPDLVRAV"
     gene            3250..>4167
                     /locus_tag="VNU03_17910"
     CDS             3250..>4167
                     /locus_tag="VNU03_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76400.1"
                     /translation="MVAPPVDPEVADAARRRRVAAGAERRRETTAPRPPRRSDVALRI
                     ALDRIMGQLPPGTFLAPEDEVAASLSDPGYLLIPGPLVVTQLSEGVALVAWSDIVDQF
                     PPRLIGLGSAEISEHLGDGLRLPLDEVVGQLPHELFVADTPEVELSGLDRIPVPFHPL
                     EESEPTPALTARIASEVRPAPPTERPVVATSPIVTERPVVELPAEPRVQPAPAPVLEP
                     PGRIEEPSLSVPEPDPLPKPVHVPEPEPVAARVPVSQIDEPTVRISFGRVMADLPAEG
                     FHGALDQLGARMQHPGALLIPLAQVLPQLG"
BASE COUNT          561 a         1481 c         1464 g          661 t
ORIGIN      
        1 caccgcggtg agcgtgatgc gtccggtcgc gccctccgag atcgtgaccg cgagcgatcg
       61 ggagtcggtc cggctggacg agcaggtgct ggacctcgtc cgactcgacc gggtcctggg
      121 gctcaaggcc acgccgccgt cgatgcggtt gccggtggtc gtcctgcgtg gcggtgggaa
      181 gccgttcggg gtggtcgtcg acgagctgct cgggaaggag gagatcgtca tcaagagcct
      241 cggggcgctg ctcgagggcg tgggcccgtt ctcaggggcc acgatctccg gcgaagggcg
      301 agtcatcctg ctcctcgacc cgacccggct gcgtgacgcc gcgacgactt ctccgacgat
      361 gccgacggcc acgtcccgcg aggaagagcg ggcggacgag acccgctcgc gggtcctgct
      421 ggtggacgac tcggtcagca tccgcaagtt cgtcggacag atgctggaga aggccggctt
      481 cgacgtgctc accgcggtgg acggccagga tgcgatccag cagctcaccg agcagacggt
      541 cgacgcggtg atcaccgacc tcgagatgcc gcgcgtgaat ggctacgcgc tgatcgagga
      601 tctccgtcgc cgctccacca ctcgcgagat cccggtcatc gtgctgacca cgcgggccgg
      661 tgccaagcac gtcggcctcg cgcgtcgact gggtgtccgg cactacgtcg ccaagccggt
      721 ggaagagcag agcttcatcc ggctcgtggc ctcggtcacg agcgtgccgg aagcggtcct
      781 cgcggtggcc gggcgctccg gaacgatggc ggactagccg gtggccaacg cgaaggtgct
      841 cgtggtggat gccaaccagg acagccgcgc ggcccttgcc cacgccctga ccgaggccgg
      901 ctacgaggcc gcagtggcca ccagcggatc gttcgcgctg accatgctgg aatgggaaca
      961 gcccgacctg atcgtgagct acgccaaggt gcaggacatg gacggctacg agctgttcac
     1021 gatggtccgc cgcgatccgg ccacgaagga caccccgttc ctcctgctgg ccgggcgcga
     1081 tcggcccatc gcgctggctg cgacggaggc cggggtggac atgaccgtga ccggagactt
     1141 cacgctggac accgtcatga cccgagtcca ggagcttctg ggcgacgtgc cggcgtccgc
     1201 gcctcccaag cctcgttccg agccgccgcc cgccgtgtcg aaggcgccgg ccaagatgcc
     1261 ggcccgacca cccgcggtca ccgccgcccc gatgcccgcg cccgccgcgg cgaccgtccc
     1321 gcctccgccc gcgccccagc cgaagccccc ggtcgaggcg gcgcgcagca gcttccaggg
     1381 ctcgctcgga gtgatggatc tcaccgagct gacccaggtg atctccctcg gcgtcaagac
     1441 cggcgagctc cacctctcac tgtcgaaggg ggaggggatc atcctgttcg aggcgggccg
     1501 ggtggtgcac gccgagttcg cggagcgatc tggcgaagcc gcgttcggcg cgatggtcgc
     1561 cgcctcccag cgcgaaggtg aggcgcgatt ctgcttcaac cgggcggacc gtgaggccat
     1621 ggccggccgg cctcggacca tctcgcgctc ggtcgagcag ctgctcctga gcgtagccca
     1681 ggagatggac gagatgagcc acggcgctgg agggggccgg gagccggccg ccactcgcgc
     1741 gacacgaacg ggagaagcct aggtcatgcc caaggtactg gtggtcgacg acagcttgag
     1801 cgtccgcaag gtggtgcagc gagcgctcga gtcccgacgg atcgaggtgc tgtccgcggc
     1861 ctcgggtacc gaggccctcg agcagatcgg tcgcgaggcg cccgacctga tcgtctgcga
     1921 cgtcatcatg ccggacatgg acggctacca gatctgcgac ttcgtcaaga aacacccgac
     1981 gctcggtcac accccggtgc tcctgatctc gggcatcgtc aacggcacgg tgctcgagcg
     2041 cgcggccaag gtccgctccg acgacgtgat gcggaagccg ttcgcggccg aggagctgtt
     2101 gcagaggatc gagagcttcc tgaacgcgca gagccggact ccgtcctcga ccccgccacc
     2161 ggccgcggtc acggcacccg tggagcgtcc cgcccccgcc gcggtcacgg cacccgtgga
     2221 gcgtcccatc ccggtcccgg tgatcgaccg gcctgccgtc gtcctcgaga gcgcggcgcc
     2281 ctcggtggac ctgaaggtcc tcctcgacgg ggtggctcag ctgccgggtg tatcgttgac
     2341 agcgctgatc gaccgtgaag gcgcgctcat ggaagcggcc ggtgggttgc tccccgaggc
     2401 gacgctcgcc ggtgccctgg cggcctgcct cgtcgattcg acagtcggag tcgggcaagc
     2461 tttggctcaa ggtcgtctgc agagtgtgat cctcgaatac gacgccgggg tggtcctgct
     2521 caatgcggtg ggcgagggcg cgatgctcgc cgtggtcctc ggtgactcgg ccgtcctcgg
     2581 taaggtccgc taccacgtga agaaggccct tccggacctg gtccgggcgg tgtgaggcat
     2641 tcgtccatga gaacgtccta cagctcggtc tccagtgccc ggcggctctc gtcgtggctg
     2701 atggtcatcc tggggctggc cgcgtacgtc ggcctcctct acgggttgac catgctgccc
     2761 ctgcaattcg aagtgccgct gccgcactgg gggatcgcgg tggtccctcc gctcgtctac
     2821 ggtctcctcg tcctgctctg cgtgcgctgg ccgtctccgc tccggtggct cgtcggcacg
     2881 gccctcctga gcggcctcca cctcctgctc ggcatggccc gcggtcccgt ttccgcgttc
     2941 ctcgatcctt ccctggcggg ccggccgctg ccctgggtgc ttcccccacc gctcccggag
     3001 ctggtgggtc tcatcctgct cctggtgccg ctccacgacc tgcttcgagc gcgtccgcgg
     3061 tctgctcgcg agcgagtgtc ggcggccggg cgcgtggcgt cctcctctcg gggccggacc
     3121 ctctcggcgc gaccgctgca gcccgtttca ggggaaggcc ggcaggcgcc gggcgagacc
     3181 ctggtcgcgc cgcaggtcga ggtttccccg gagacgacca ctcccgcgac cccggtcgcc
     3241 cctccaatca tggtggcgcc gcccgtcgac ccggaagtgg cggatgcggc gcgccgccgc
     3301 cgggtggcgg ccggggccga gcgccggcgt gagacgacgg ctccgcgccc gccccgccgg
     3361 tcggacgtcg cgctgcgaat cgccctcgat cggatcatgg gccagctgcc gccgggcacg
     3421 ttcctcgcgc ccgaggacga ggtcgcggcg agcctcagcg atcctggata tctgctcatc
     3481 cccggaccgc tcgtcgtcac ccagctctcg gaaggcgtcg cgctggtcgc gtggagcgat
     3541 atcgtcgacc agttcccccc gaggctcatc ggactcggga gtgccgagat cagcgagcac
     3601 ctcggagatg gactgcggct gccgctcgac gaggtggtcg gtcagttgcc gcacgagctg
     3661 ttcgtcgcgg acactcccga ggtcgagctg tcgggtctcg atcgcatccc ggtgccgttc
     3721 catcccctcg aagagtccga gccgacgccg gccctgacgg cccggatcgc ctccgaggtc
     3781 cgtcccgcgc caccgaccga gcggccagtt gtcgcgacat cccctatcgt gacggagcgg
     3841 cccgtcgtcg agctcccggc cgagccgagg gtccagccgg cgccggctcc cgttctcgaa
     3901 ccgccggggc ggatcgagga gccgtctctt tccgtccccg agccggatcc gctgccgaag
     3961 ccggtccacg ttcccgagcc ggagccggtg gctgctcggg tgcccgtctc ccagatcgac
     4021 gagcccacgg tccgcatttc cttcggccgc gtgatggccg accttccggc ggaagggttt
     4081 catggggcgc tcgatcagct gggggcgcgg atgcagcatc cgggtgcgct gctcattccg
     4141 ttggcgcagg tgctgccgca gctgggc
//
LOCUS       DATMCV010000781         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_40255, whole genome shotgun sequence.
ACCESSION   DATMCV010000781 DATMCV010000000
VERSION     DATMCV010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40255"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VNU03_17915"
     CDS             complement(<1..695)
                     /locus_tag="VNU03_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(138..140),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HWP76401.1"
                     /translation="MSAGATPGAAAPRIQFAPEDDVAIRYMDRTREYYQALGFSPYRW
                     AHFTDAPFTPLRVPLAKARGALITTTAPFQPEAGDQGPRAPYNAAAKFYQVYSAPVDS
                     KPDLRISHVGYDRANTVPEDLDAYVPLARLQEAVAAGRIGGLGPRFHGAPTNRSHRVT
                     METDAPELLRRCREDGADLAVLVPSUPVCHQTVGLVARHLEANGLSTVIMGCAKDIVE
                     HCGVPRFVWSDFPL"
     gene            complement(692..1642)
                     /locus_tag="VNU03_17920"
     CDS             complement(692..1642)
                     /locus_tag="VNU03_17920"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HWP76402.1"
                     /translation="MSFDYGKYFGEIKLTHDLLSKKEDRTWLLKPPRDGERHDVVLYL
                     GCNVLRTSHLVETVGAVFDRLGLDYVAAGGPTYCCGIQHHRRGQEASGERYANHTVEL
                     LQSMAPREVVMWCPSCIQFYDEVLQADLPFPKRHTAEFLADCLDRGLFTFTQRVEATV
                     ALHYHYATEARAREGRAARRLLEAVPGITVVDFEPDPRWGRTCTPALQEQLGPEVWRQ
                     MALDDIDRARAAGATHLAGIYHGCHRDQCRFELERPIVVEHYLTLFARALGIEFEDTY
                     KKYMHMGDPDRILEDASPCMAANGVDPEKARGLVIKTFSA"
     gene            complement(1671..2924)
                     /locus_tag="VNU03_17925"
     CDS             complement(1671..2924)
                     /locus_tag="VNU03_17925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP76403.1"
                     /translation="MATDRIVIVGASLAGATASAALRDGGFEGELTLIGEEARLPYSR
                     PALSKGYLRGQERFEDQLVNPADYYDKHRITLRLGARATRVDPARRVVELGGGETLPY
                     DRLLVTTGGRNRTLRTPGASLSGIFQLRTVEDCDRIRAAARSGARAVVVGMGFIGSEV
                     TASLRQMGVTVSAIEGHPVPLARVLGPEVGAVLAAIHRDHGVELVLGDSVAAFEGTGR
                     VERVRTAKGRVLDCDLVVAGIGIEPNQELLAAAGALVDNGVLVDPHCRTSLPEVYAAG
                     DVANHQHPLFGRLRVEHWNNGLQHGWAAARAMLGGSEPYDYVHSFWSDQYEHLIEYVG
                     FAATWDRLVFRGTPESRKFLGFYLKDGVLRAAVGLDRGGDPEDPKSESELKVVAGLIR
                     SRARVDPARLVDEAVELSRLAPSPA"
     gene            complement(3004..>3602)
                     /locus_tag="VNU03_17930"
     CDS             complement(3004..>3602)
                     /locus_tag="VNU03_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76404.1"
                     /translation="GFVCWTRRGLMTITSRNGQSEGVLMAGQRAWARQGWPVTPEPFV
                     AGASTLEIESSGPVMPLLRMPDGRVAAGFLADGVEVNQVFGSLSASRGGQRWLVEVPG
                     GAEGAYTLILTGAGTGPFTARVSARYAGFRIYRQELTGEIRPGEQLVTRINQRVKGDD
                     PSTARVVAGSVEGLRAWDGDEPAAVVAAPGARRFPGAN"
BASE COUNT          500 a         1317 c         1288 g          497 t
ORIGIN      
        1 agggggaagt cgctccacac gaagcgcggg acgccgcagt gctccacgat gtccttggcg
       61 cagcccatga tcacggtcga caggccgttc gcctcgagat gccgggcgac cagacccacg
      121 gtctggtggc agaccggtca gctggggacg aggaccgcca ggtcggcgcc gtcctcgcgg
      181 cagcgtcgca gcagctcggg cgcgtcggtc tccatggtca cgcgatgact ccggttggtc
      241 ggcgccccgt gaaagcgcgg gcccaggccg ccgatgcgtc ccgccgcgac cgcttcctgc
      301 agccgggcca gcgggacgta ggcgtcgagg tcctcgggga ccgtgttcgc ccggtcgtag
      361 cccacgtgcg agatccggag atcgggcttg ctgtccaccg gggccgagta gacctggtag
      421 aacttcgccg ccgcgttgta cggcgcgcgc gggccctggt cgcccgcctc cggctggaac
      481 ggcgcggtgg tggtgatcag cgcgccgcga gcctttgcga gcgggacgcg cagcggggtg
      541 aacggcgcgt ccgtgaagtg ggcccagcgg tacgggctga agccgagcgc ctggtagtac
      601 tcgcgggtgc gatccatgta ccggatcgcc acgtcgtcct ccggagcgaa ctggatccgg
      661 ggagccgccg ccccgggagt ggcgcccgcg ctcatgccga gaaggtcttg atgacgagcc
      721 cgcgcgcctt ctcgggatcg accccgttgg ccgccatgca ggggctcgcg tcttccagga
      781 tccggtcggg atcgcccatg tgcatgtact tcttgtaggt gtcctcgaac tcgatgccga
      841 gggcgcgggc gaagagcgtc aggtaatgct cgaccacgat gggccgctcg agctcgaatc
      901 ggcactgatc gcggtggcag ccgtggtaga tgccggccag gtgcgtggcg ccggccgccc
      961 gcgcccggtc gatgtcgtcg agcgccatct gccgccagac ctcgggtccg agctgctcct
     1021 ggagcgccgg cgtgcaggtc cggccccagc gaggatccgg ctcgaagtcg accaccgtga
     1081 tcccgggtac cgcctcgagc agccgtcgcg cggccctgcc ctcgcgcgcg cgggcctcgg
     1141 tcgcgtagtg gtagtggagc gccacggtgg cctcgacccg ctgggtgaac gtgaagagcc
     1201 cccggtcgag gcagtcggcg aggaactcgg cggtgtggcg cttcgggaac ggcaggtcgg
     1261 cctggagcac ctcgtcgtag aactggatgc acgaggggca ccacatgacg acctcgcgcg
     1321 gggccatgga ctgcagcagc tcgacggtgt ggttggcgta ccgctcgccc gaggcctcct
     1381 gcccgcgccg gtggtgctgg atgccgcagc aataggtcgg cccgcccgcg gccacgtagt
     1441 cgagcccgag gcggtcgaac accgcgccca ccgtctcgac caggtgcgag gtgcgcagca
     1501 cgttgcagcc caggtagagg accacgtcgt ggcgctcccc gtcccgcggc ggcttgagca
     1561 gccaggtccg gtcctccttc ttcgagagga ggtcgtgggt cagcttgatc tcgccgaagt
     1621 acttgccgta gtcgaagctc atggtgtcga gagtttacca ccaagggggc tcaggcgggg
     1681 ctcggcgcca ggcgcgacag ctccaccgcc tcgtcgacca ggcgggccgg atcgacgcgg
     1741 gcgcggctcc ggatcaggcc ggccacgacc ttgagctcgc tctcggactt cggatcctcc
     1801 ggatccccgc cccggtcgag cccgaccgcc gcgcgcagca cgccgtcctt caggtagaag
     1861 ccgaggaact tccggctctc cggcgtgccc cggaacacga ggcggtccca cgtcgcggcg
     1921 aagcccacgt actcgatcag gtgctcgtac tgatccgacc agaacgagtg gacgtagtcg
     1981 tagggctcgc tcccgcccag catcgcgcgc gcggcggccc aaccgtgctg caggccgttg
     2041 ttccagtgct ccacgcgaag gcggccgaag agcgggtgct gatggttcgc cacgtcgccc
     2101 gccgcgtaca cctccggcag cgacgtgcgg cagtgggggt cgaccagcac gccgttgtcg
     2161 acgagggcgc ccgccgcggc cagcagctcc tggttgggct cgatcccgat gcccgcgacg
     2221 acgaggtcgc agtcgagcac tcgccccttg gcggtgcgta cccgctcgac gcgcccggtg
     2281 ccctcgaacg cggcgaccga gtcgccgagc accagctcga ccccgtggtc ccgatggatg
     2341 gcggccagca ccgcgcccac ctcgggcccc aggacgcgcg cgagcggaac cggatggccc
     2401 tcgatcgcgg acaccgtcac gcccatctga cgcagggacg cggtcacctc ggagccgatg
     2461 aagcccatgc cgaccacgac cgcacgggcc ccggagcgcg ccgccgcccg gatccgatcg
     2521 cagtcctcca cggtgcggag ctggaagatg cccgagaggc tcgcgccggg cgtgcggagc
     2581 gtgcgattgc ggccgccggt ggtcaccagc agccggtcgt agggcagcgt ctcgccgccg
     2641 cccagctcga ccacgcggcg ggccggatcc acgcgggtcg cgcgcgcgcc gagccggagc
     2701 gtgatgcggt gcttgtcgta gtaatccgcc gggttgacga gctgatcctc gaagcgctcc
     2761 tggccgcgga gatagccctt cgagagcgcc ggccggctgt agggcagccg ggcctcctcc
     2821 ccgatcagcg tcagctcgcc ctcgaagccg ccgtcacgca gcgccgccga cgcggtggcc
     2881 cccgccagcg acgcgccgac gatcacgatc cgatcggtcg ccatggtctc gtgtcccccg
     2941 ggttcattga acgcccgatc gggccgcctg tccaagactc gctcggtccg ctccgcggcg
     3001 cgatcagttc gcgccaggga agcgtcgggc gcccggggcc gcgaccaccg ccgccggctc
     3061 gtcaccgtcc cacgcgcgca ggccctcgac gctgcccgcc accacgcgcg cggtactcgg
     3121 atcgtcgccc ttgacccgct ggttgatccg ggtgacgagc tgctcgccgg gccggatctc
     3181 gccggtcagc tcctgccggt agatccggaa ccccgcgtag cgcgcggaga cgcgcgcggt
     3241 gaacggaccc gtccccgcgc cggtgaggat cagcgtgtag gcgccctcgg cgccccccgg
     3301 cacctcgacg agccaccgct ggccgccccg gctcgcgctg agcgagccga acacctggtt
     3361 gacctcgacg ccgtcggcca ggaagccggc cgcgacgcgg ccgtccggca tccgcagcag
     3421 gggcatgacc gggccggacg actcgatctc gagcgtgctc gccccggcga cgaacggctc
     3481 cggggtgacg ggccagccct gccgcgccca cgcgcgctga ccggccatca gcacgccctc
     3541 ggactgcccg ttgcggctgg tgatggtcat caagccgcgg cgcgtccagc acacgaagcc
     3601 ct
//
LOCUS       DATMCV010000782         3414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_378660, whole genome shotgun sequence.
ACCESSION   DATMCV010000782 DATMCV010000000
VERSION     DATMCV010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378660"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..970
                     /locus_tag="VNU03_17935"
     CDS             101..970
                     /locus_tag="VNU03_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP76405.1"
                     /translation="MLQQQVGDITIHRILESERPDFDAAGFFPQITPEQWAPYRQRLA
                     GWALDPAVNGLVFPMQSFLVKTRHHTIVVDTCVGDHKERARANWNMTLSGAYLARLAE
                     AGVRPEQVDYVMTTHFHTDHVGWNTRWDNGRWVPTFPNARYVMSEKEWAYWSAMHKET
                     PQNQIADSVIPIVESGLAQMVKNDFALDDEVRFESTWGHTPDHMSVHIASRGRHAVIT
                     GDLIHSPVQCLETDWVPRPDFDPKQAAATRRAFLDRYCEQDVLVCASHFPSPSFGRVV
                     REGNGFWFEYVKA"
     gene            984..2012
                     /locus_tag="VNU03_17940"
     CDS             984..2012
                     /locus_tag="VNU03_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76406.1"
                     /translation="MTARKKNHDWLALTVEEPIEPALPICDPHHHLWDGETGRVVPRY
                     LLDEILEDVGGGHRIVSTVFIECGAMFKPDGPEALRAVGETEFVNGIAAMSASGLYGP
                     TRIAAGIVGTAPLRLGAAAAPVLDAQIAAGGGRFRGIRLGAAWDADPAVGNHRTRPPQ
                     GLFLRDDFRAGFALLAPRGLTFEAWCYHRQIPDVTALARAFPDTTIILDHFGGPVGVG
                     SYAGRGREVHAEWRGYISELATCPNVMAKLGGLNMEVNGFGWHEQPRPPSSQKLADAT
                     RPYYEFTIEKFGVDRCMFESNFPVDAVSCSYTVLWNTFKHLTAGYSAAEKAKLFHDTA
                     TRVYRLAT"
     gene            complement(2005..2418)
                     /locus_tag="VNU03_17945"
     CDS             complement(2005..2418)
                     /locus_tag="VNU03_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWP76407.1"
                     /translation="MHPARPRGSLTTLAALVLLAVGAGGCASGGMTDRVTPQALLAAI
                     DAGTAPVIVDVRTRREYEAGHVPGAIHVPFYTLLVRQDDVPGPRERPVVVYCEHGPRA
                     GVARLALRMTGFTDVRYLDGHMAGWKKSGLREELR"
     gene            2683..>3414
                     /locus_tag="VNU03_17950"
     CDS             2683..>3414
                     /locus_tag="VNU03_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76408.1"
                     /translation="MTAPIRRRPPFVSLVGLALLLASLTATVPGDATAADTPRRGGVL
                     LAVIGADPPSLDPHQESTFANIELVAPLYSTLLQLDPYSYPKIIGDLATEWKISPDGL
                     TYTFKLHPGVKFHDGSPLTSADVKASYDKIIFPPAGVRSVRQNAYTAVSQVEAPDPAT
                     VVFKLKFPSASLLANLASPWNVIFPKSYLDRDPNYFKTHAIGSGPFKFKGYTRGSTFE
                     GERNPAYFVKDRPYLAGYKFFISPET"
BASE COUNT          517 a         1284 c         1110 g          503 t
ORIGIN      
        1 ttcctgaagg acgtccgcgc caaggtcggc gcggccgggg gcctcgactg gacccgcgtg
       61 gacgagatcg atttctcgaa gctctaagga ggcctccctc atgctgcagc agcaggtcgg
      121 cgacatcacc atccaccgga tcctcgagtc ggagcgcccg gacttcgacg cggcgggctt
      181 cttcccgcag atcacgcccg agcagtgggc gccgtaccgg cagcggctcg cgggctgggc
      241 cctggacccg gcggtgaacg gcctcgtctt cccgatgcag tcgttcctgg tcaagacccg
      301 ccaccacacc atcgtggtgg acacctgcgt gggcgaccac aaggagcgcg cgcgcgccaa
      361 ctggaacatg acgttgagcg gtgcctatct cgcccgcctc gccgaggcgg gggtgcggcc
      421 cgagcaggtc gactacgtca tgaccaccca cttccacacc gaccacgtgg ggtggaacac
      481 acgctgggac aacggccgct gggtgcccac ctttcccaac gcccgctacg tcatgtccga
      541 gaaggagtgg gcgtactggt cggccatgca caaggagacc ccgcagaacc agatcgcgga
      601 cagcgtgatc cccatcgtgg agtcgggcct ggcccagatg gtgaagaacg acttcgccct
      661 cgacgacgag gtgcgcttcg agtcgacctg ggggcacacc cccgaccaca tgagcgtgca
      721 catcgcgtcc cggggccggc acgcggtgat caccggcgat ctcatccact ccccggtgca
      781 gtgcctcgag accgactggg tgccgcggcc cgacttcgac cccaagcagg ccgcggccac
      841 ccgccgcgcc ttcctcgacc gctactgcga gcaggacgtg ctggtgtgcg cctcgcactt
      901 cccctcgccc tccttcggcc gcgtggtgcg cgagggcaac gggttctggt tcgagtacgt
      961 gaaggcctga gcgcggaccg gtcgtgaccg cccggaagaa gaaccacgac tggctcgccc
     1021 tgaccgtcga ggagccgatc gagcccgcgc tccccatctg cgatccgcat catcacctgt
     1081 gggacgggga gaccggccgc gtcgtcccgc gctatctcct ggacgagatc ctggaggacg
     1141 tcgggggcgg ccaccgcatc gtctccaccg tgttcatcga atgcggcgcg atgttcaagc
     1201 ccgacgggcc ggaagccctc cgcgcggtcg gggagaccga gttcgtgaac ggcatcgccg
     1261 cgatgagcgc ctccggcctc tacggtccca cgcgcatcgc ggccgggatc gtggggaccg
     1321 cccccctgcg cctgggcgcc gcggcggccc cggtgctcga cgcgcagatc gcggccgggg
     1381 gcggccgctt ccgcggcatc cgcctcggcg cggcgtggga cgccgacccc gcggtgggaa
     1441 accaccgcac ccgtcccccg cagggcctct tcctgcgcga cgacttccgg gccggcttcg
     1501 ccctgctggc gccccgtggg ctcaccttcg aggcgtggtg ctaccaccgc cagatccccg
     1561 acgtcaccgc gctcgcccgc gccttcccgg acaccacgat catcctcgac cacttcggcg
     1621 gcccggtcgg ggtcggctcc tacgcgggcc gggggcgcga ggtccacgcg gagtggcgcg
     1681 gctacatcag cgagctggcc acctgcccca acgtgatggc caagctgggc gggctcaaca
     1741 tggaggtcaa cggcttcggc tggcacgagc agccccggcc gccttcgagc cagaagctgg
     1801 ccgacgcgac gcggccgtac tacgagttca ccatcgagaa gttcggggtg gatcgctgca
     1861 tgttcgagtc gaacttcccg gtggacgcgg tgagctgcag ctacaccgtg ctgtggaaca
     1921 ccttcaagca cctgaccgcg ggctactcgg cggccgagaa ggccaagctc ttccacgaca
     1981 ccgccacccg ggtctatcgg ctggctacct gagctcctcg cgcaatccgc tcttcttcca
     2041 gcccgccatg tgcccgtcga ggtagcggac gtccgtgaag ccggtcatcc gcagcgcgag
     2101 ccgggccacc ccggcgcgcg gcccgtgctc gcagtacacc accaccggcc gctcgcgggg
     2161 gcccgggacg tcgtcctgcc ggacgaggag cgtgtagaag ggaacgtgga tggcgcccgg
     2221 cacgtgaccc gcctcgtact cgcgacgcgt ccggacgtcg acgatcaccg gcgcggtgcc
     2281 ggcatcgatc gcggcgagca gcgcctgcgg cgtcacccgg tcggtcatgc ccccgctcgc
     2341 gcagccgccc gcgcccaccg ccaggagcac gagcgcggcg agcgtggtca gggatcctcg
     2401 aggccgcgcg ggatgcatgg ccggatggta gcgcaggccg gcgctccgcg tcccggctcc
     2461 gcggttgaca ggtccgtctc gcccctggta cggtccgcgt gttcgagcgt gcgaaccgtc
     2521 cgagaccgct tcggcccccc ttaagacggc gcaagcgcag gtcgtccagg tcggcttccc
     2581 gaggcggggg cccctatcca ggcccgggga agtgcgtatg cctcgcaggc gaagtccttc
     2641 cgacgtcatc ccccagcatc cgactcgtgc gggaggtgct ccatgaccgc tcccatccgg
     2701 cgtcgtcccc cgttcgtctc cctcgtcggg ctcgccctgc tcctggcctc gctcaccgcg
     2761 accgtgcccg gcgacgcgac ggccgccgac acgccccggc gcggcggcgt cctgctcgcg
     2821 gtgatcggcg ccgatccgcc gagcctcgac ccccaccagg agagtacgtt cgccaacatc
     2881 gagctggtgg cgccgctcta cagcaccctg ctccagctcg atccctacag ctatccgaag
     2941 atcatcgggg acctcgcgac cgagtggaag atctcgcccg acgggctcac ctacacgttc
     3001 aagctccacc cgggggtgaa gttccacgac gggtccccgc tcacctcggc cgatgtgaag
     3061 gccagctacg acaagatcat cttcccgccc gcgggggtcc gtagcgtgcg gcagaacgcc
     3121 tacaccgcgg tcagccaggt cgaggccccc gatccggcca ccgtggtgtt caagctcaag
     3181 ttcccgtccg cgtcgctcct ggccaacctg gcctcgccct ggaacgtgat cttcccgaag
     3241 agctacctcg accgggatcc gaactacttc aagacccacg cgatcggcag cggccccttc
     3301 aagttcaagg gctacacccg cgggtccacc ttcgagggcg agcgcaatcc ggcctacttc
     3361 gtcaaggacc gcccgtatct cgccggctac aagttcttca tcagcccgga gacc
//
LOCUS       DATMCV010000783         3231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_308705, whole genome shotgun sequence.
ACCESSION   DATMCV010000783 DATMCV010000000
VERSION     DATMCV010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308705"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1092)
                     /locus_tag="VNU03_17955"
     CDS             complement(67..1092)
                     /locus_tag="VNU03_17955"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HWP76409.1"
                     /translation="MPGPKIAAVATATPPWRYDQATVLRMSGYDDPRRMGFFSNSLIE
                     TRHLYMDPETFTPDESVDQLNDRWRRGAVEVGKAAVARAIEQAGWRREDVDFLATTTC
                     TGRVCPSLDAQIINELGLKSTIQRVHVGDTGCASAMVAMQQVSNHLRAFPDHRAVMVA
                     VEICSAAYFLDDRLESAVAHAIFADGAGAIAISRDGAGPEIVEHRTMFRSEHLGAMGF
                     EYPGGRPRVVLSKDVRRIGAGMMKEMADALMADNGLKTEDIAHFVLHSAGRRVIEQVA
                     KLMTLDETRLAHSRSVLQQYGNMSSATVVFVLDDLLRSRQPVPGDWGLMIALGPGFAA
                     EGALLRW"
     gene            1255..1779
                     /locus_tag="VNU03_17960"
     CDS             1255..1779
                     /locus_tag="VNU03_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015628998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWP76410.1"
                     /translation="MPLFSFEGKSPRVHPTAFIAPTAILIGDVTVEENASVWYNTVLR
                     ADFNPIVIRRGANVQDCAVIHVTPAGGVEIGPGATVGHLCLVHAATVGEEALVGNSST
                     LLDGVKIGVRAMVAAGSLVTPGTEIPDGMLAMGAPCKVKGPLAGTAAERWVQMNPAGY
                     QALAQRHRKGVQPA"
     gene            1776..2408
                     /gene="pcp"
                     /locus_tag="VNU03_17965"
     CDS             1776..2408
                     /gene="pcp"
                     /locus_tag="VNU03_17965"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HWP76411.1"
                     /translation="MTPDPARHLLLTGFEPFDGDPVNPSGEVAKHLDGRVIGDCVVRS
                     AILPVQHEAARAMVAPRLDAPGLVAIVHLGLAGGRARVSLERVAVNVMDYSRPDAHGQ
                     VLRGVACAEDGPAAYFSTLPLRAMLAALTAEGIPAAISNTAGTYLCNDISYTTLHVLA
                     RRGLAIPTGFIHLPFLPSMVSAHDLEEPSMDLPLMVRAVEIALQHARPAR"
     gene            complement(2412..>3231)
                     /locus_tag="VNU03_17970"
     CDS             complement(2412..>3231)
                     /locus_tag="VNU03_17970"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HWP76412.1"
                     /translation="AWALGRLGAAERPVLRGAVFGGVGAGIAAAGLLCVALMERGAGS
                     ASAWIQLGALALAGTAAVWPALREDGAAAGAPAPRRAAGRFRWDRDAVRLVLCHGILG
                     LGYIIPATFVPAMARTLVPDPLVFGWAWPIFGVAAMASTLGAGAAIRVVGNRRLWALS
                     QLVMAAGVAAPVLWPSLGGIMVAALLVGGTFMVSTMGGMQEAQIVAGPHATRLMAAMT
                     AAFAAGQIAGPLLVATAVGPHGDFSRPLSIACLLLLVSAGALARRVGPGPGSGR"
BASE COUNT          453 a         1258 c         1109 g          411 t
ORIGIN      
        1 ggcagcggcc cgtcgatgat ctcgagcacg cccgcggcac gcgccagcgg agggaagagc
       61 ggcatcctac cagcggagca acgcgccctc ggccgcgaag cccggtccca gggcgatcat
      121 gagtccccag tcgcccggca ccggctggcg cgaccgcagc aggtcgtcga gcacgaagac
      181 cacggtggcg gacgacatgt tgccgtactg ctgcagcacg ctccgcgagt gggcgagccg
      241 cgtctcgtcg agcgtcatca gcttcgccac ctgctcgatc acccggcggc ccgcggagtg
      301 cagcacgaag tgggcgatgt cctccgtctt gagcccgttg tccgccatga gcgcgtcggc
      361 catctccttc atcatgcccg ccccgatgcg ccgcacgtcc ttggagagca ccacccgcgg
      421 ccgcccaccc gggtactcga agcccatcgc ccccaggtgc tcggagcgga acatggtgcg
      481 gtgctcgacg atctccggtc ccgccccgtc ccggctgatc gcgatggccc ccgccccgtc
      541 cgcgaagatc gcgtgcgcca ccgcgctctc gagccggtcg tcgaggaagt aggccgccga
      601 gcagatctcc accgccacca tcaccgcgcg gtggtcgggg aacgcgcgca ggtgattgga
      661 cacctgctgc atcgccacca tcgcgctggc gcagccggtg tcgcccacgt gcacgcgctg
      721 gatggtcgac ttgagcccga gctcgttgat gatctgcgcg tcgaggctcg ggcacacccg
      781 cccggtgcag gtggtggtgg cgaggaagtc gacgtcctcc cgccgccagc ccgcctgctc
      841 gatggcgcgg gccaccgcgg ccttgcccac ctccaccgcc ccgcggcgcc agcggtcgtt
      901 gagctggtcc acgctctcgt cgggggtgaa cgtctccgga tccatgtaga ggtggcgcgt
      961 ctctatcaaa ctattgctga aaaatcccat gcgccgcggg tcgtcgtagc ccgacatgcg
     1021 cagcacggtg gcctgatcgt aacgccaggg cggcgtggcg gtggcgacgg cggcgatctt
     1081 cggcccgggc atgatgggcg caggctaccc cccgccccca accttgtcca ccggcccgga
     1141 ccgccacgtg ccttggcggc ccgcacttgc cttggccgcc cgcacttgcc ttggccggag
     1201 gacgggggca atatcgtgcc tcgcagccat ccccacgtcg gcaaggagtc gcggatgccg
     1261 ctcttctcgt tcgaaggcaa gtccccgcgc gtgcacccca ccgccttcat cgcgcccacc
     1321 gcaattctca tcggcgacgt cacggtggag gagaacgcct cggtctggta caacaccgtg
     1381 ctccgcgccg acttcaaccc gatcgtgatc cgccggggcg ccaacgtgca ggactgcgcg
     1441 gtcatccacg tgaccccggc cggcggcgtc gagatcggcc ccggcgccac cgtcggacac
     1501 ctctgcctgg tacacgcggc caccgtcggc gaggaagcgc tggtgggcaa ttcctcaacc
     1561 ctgctcgacg gcgtcaagat cggcgtgcgc gccatggtgg ccgcgggctc gctcgtgacg
     1621 ccgggcaccg agatccccga cggcatgctg gccatgggcg ctccctgcaa ggtgaagggc
     1681 ccgctggccg gcaccgccgc cgagcgctgg gtgcagatga acccggcggg ctaccaggcc
     1741 ctcgcccagc gccatcgcaa gggagtgcag cccgcgtgac ccccgacccg gcccgccacc
     1801 tcctgctcac cggcttcgag cccttcgacg gcgacccggt caacccgtcc ggcgaggtgg
     1861 ccaagcacct cgacggccgc gtgatcggcg actgcgtggt ccggagcgcg atcctgccgg
     1921 tgcagcacga ggcggcgcgc gcgatggtgg ctccgcggct ggacgcgccg gggctggtgg
     1981 cgatcgtgca cctgggcctg gccggcggcc gcgcgcgcgt ctcgctcgag cgcgtggcgg
     2041 tcaacgtgat ggactactcg cggcccgacg cccacgggca ggtgctgcgc ggggtcgcct
     2101 gcgcggagga cgggccggcg gcctacttca gcacgctgcc gctgcgggcg atgctggccg
     2161 cgctgaccgc ggagggcatc ccggccgcga tctcgaacac cgccgggacc tatctctgca
     2221 acgacatctc ctacaccacg ctgcacgtcc tggctcgacg cggcctcgcg atcccgaccg
     2281 gcttcatcca cctgccgttc ctgccgtcga tggtgagcgc gcacgatctc gaggagccga
     2341 gcatggatct gcccctgatg gtccgcgcgg tcgagatcgc cctgcagcac gcccgtccgg
     2401 cgcgctgacc ctcagcgtcc cgagcccggt cccggcccca cgcgacgggc cagcgcgccc
     2461 gcgctcacca gcagcaacag gcacgcgatc gacagcggcc gcgagaaatc gccgtgcggc
     2521 ccgacggcgg tggccaccag gagcgggccg gcgatctggc ccgccgcgaa cgcggcggtc
     2581 atcgcggcca tcagccgtgt cgcgtgcggc cccgcgacga tctgcgcctc ctgcatgccg
     2641 cccatggtgc tcaccatgaa ggtgccgccc acgagcagcg cggccaccat gatgccgccc
     2701 aggctgggcc acagcaccgg cgcggccacg cccgccgcca tgacgagttg gctcagggcc
     2761 cacagccgcc ggttccccac cacgcggatc gcggccccgg ccccgagcgt cgacgccatc
     2821 gccgcgacgc cgaagatcgg ccaggcccag ccgaagacga gcgggtcggg cacgagcgtc
     2881 cgcgccatcg cgggcacgaa ggtcgcggga atgatgtagc cgaggcccag gatcccgtgg
     2941 cagaggacga ggcgcaccgc gtcgcggtcc cagcggaagc ggccggcggc ccgccgcggt
     3001 gccggcgctc cggccgcggc gccgtcctcg cggagcgccg gccagaccgc cgcggtgccg
     3061 gccagggcga gcgcgccgag ctgaatccac gcgctcgccg atccggcgcc gcgctccatg
     3121 agggccacgc agagcaggcc ggccgccgcg atcccggcgc cgacgcctcc gaagaccgcg
     3181 ccccgcagca ccgggcgctc cgccgccccg agccggccga gcgcccacgc g
//
LOCUS       DATMCV010000784         2936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_108497, whole genome shotgun sequence.
ACCESSION   DATMCV010000784 DATMCV010000000
VERSION     DATMCV010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108497"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1424
                     /locus_tag="VNU03_17975"
     CDS             108..1424
                     /locus_tag="VNU03_17975"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76413.1"
                     /translation="MATNARKDFPVFDCDSHVVEPPAIWDEYVPARSRAWVKTQFCFH
                     TDSDLLFINGRVVPAARERSNAAEVGWARWDKKEVGKLTPGTPAWQGKFGRLLGCRDP
                     QARLKDMDALGTDQVMLFPTWFVRLALVKSAEAATVLAQAYNNWVLDYCAADRRRLFP
                     CAVLPLQSVEASIAELRRVAKLGFKAAAVRPCFWNGRYPTLAEFDPLWREFEATGTVL
                     AMHTFPSREALTPEWGERMAQARGHSGQGLLFTDESVVYSPGQFVSNITAAMDPAIDA
                     SETLGFLLEAMTWVTVVVQTGWLEKFPKLKAAVLESNASWLPLVLSRSSNFSNLFAFQ
                     RDNRLIRDPVDVFNERCFIGFESDETLVYRLWDVFQDVAIWSSDYPHHDAEDAWDGLH
                     HMAEFKVPLAAQRKLMGENARTLYGIEPLMTVEERIPEYAPAILPW"
     gene            complement(1447..2505)
                     /locus_tag="VNU03_17980"
     CDS             complement(1447..2505)
                     /locus_tag="VNU03_17980"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP76414.1"
                     /translation="MVSPRTLHTARTALWITCGWIGAALLVHWVVRRVPTHLARELTA
                     TATGAAIGLASAWFELRLIPRHVRSLTVGGLLLVRTFFYVTLCAVVIHVVTMSLLGLS
                     EHGGLLAYYTSEQYWDFVLGGRFLMALAILTLASFLINFFRQLNRMLGPGTLVSLLLG
                     RYHRPVAEERIFMFLDLNDSTAIAAALGPLRFNDFKNDFFHDIAEPILETRGRIYQYV
                     GDEAVVTWTVERGLRQGNCLRCVFLVTERIHERKDRYLARYGVVPEFKAGIHGGPVVT
                     AEIGDIKKDIVHSGDTVNTAARVEAQCRPLERRVLVSAALLDRCRVPEELELEDMGER
                     ELRGKAESLRLYSVRARA"
     gene            complement(2509..2886)
                     /locus_tag="VNU03_17985"
     CDS             complement(2509..2886)
                     /locus_tag="VNU03_17985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWP76415.1"
                     /translation="MKDIAALDRRRYVALATFRRSGAEVRTPVWFAAMDGKLYVFSAG
                     DAGKVKRLRHSPRARIAPSDARGNVRGEWWDAEARVVAEPRVIERAHAAMRAKYGWPA
                     RVGDLFSRLTGRLPRRAWIEIEI"
BASE COUNT          459 a         1004 c         1038 g          435 t
ORIGIN      
        1 acgacgcgcc ccgacagcga gaagcgctcg atccccggcg tcttgtccat gcctggcccg
       61 gataatagaa tgcccctccc gacattgaaa gggacaggag ggtcgccgtg gccacgaacg
      121 cccgcaagga tttccccgtc ttcgactgcg acagccacgt cgtcgagccg cccgcgatct
      181 gggacgagta cgtcccggcc cgcagccgcg cctgggtgaa gacgcagttc tgcttccaca
      241 ccgacagcga tctgctcttc atcaacgggc gggtggtacc cgccgcgcgc gagcgctcga
      301 acgcggccga ggtcggctgg gcccgctggg acaagaagga ggtgggcaag ctcacccccg
      361 gcacgccggc ctggcagggg aagttcgggc ggctgctcgg ctgccgcgac ccgcaggcgc
      421 gcctcaagga catggacgcg ctcggcaccg accaggtgat gctgttcccg acgtggttcg
      481 tgcggctggc gctggtgaag agcgccgagg cggcgacggt gctggcccag gcctacaaca
      541 actgggtgct cgactactgc gccgcggacc ggcggcggct gtttccctgc gccgtcctgc
      601 ccttgcagag cgtcgaggcc tcgatcgcgg agctccggcg ggtcgccaag ctggggttca
      661 aggcggccgc ggtgcgcccc tgcttctgga acgggcggta tcccaccctg gccgagttcg
      721 atccgctgtg gcgcgagttc gaggccaccg ggacggtgct ggccatgcac acgttcccgt
      781 cccgcgaagc gctcacgccc gagtggggcg agcgcatggc ccaggcgcgc ggtcactcgg
      841 gccagggcct gctcttcacc gacgagtccg tggtgtattc gcccggccag ttcgtgtcga
      901 acatcaccgc ggcgatggat cccgccatcg acgcctccga gacgctcggc ttcctgctgg
      961 aggcgatgac ctgggtgacc gtggtggtgc agaccggctg gctggagaag ttcccgaagc
     1021 tgaaggcggc ggtgctggag tcgaacgcct cgtggctccc gctggtcctg tcgcgctcct
     1081 cgaacttctc gaacctgttc gccttccagc gcgacaaccg gctcatccgc gatccggtcg
     1141 acgtgttcaa cgagcgctgc ttcatcggct tcgagtcgga cgagaccctg gtctaccgcc
     1201 tgtgggacgt gttccaggac gtggcgatct ggtcgtcgga ctatccccac cacgacgcgg
     1261 aggacgcgtg ggacggcctg catcacatgg cggagttcaa ggtgccgctc gcggcgcaga
     1321 ggaagctgat gggcgagaac gcgcgcacgc tctacggcat cgagccgctc atgacggtgg
     1381 aggagcggat cccggagtac gcgcccgcga tcctgccctg gtagggcgcc gggccgcggg
     1441 gcgggcctac gcgcgcgccc gcacgctgta gaggcggaga gactcggcct tgccgcgcag
     1501 ctcccgctcg cccatgtcct cgagctccag ctcctccggg acccggcacc ggtccaggag
     1561 cgccgccgac accagcaccc ggcgctccag cgggcgacac tgggcctcga cacgggccgc
     1621 ggtgttgacc gtgtcgccgc tgtggacgat gtccttcttg atgtcgccga tctcggcggt
     1681 gacgacgggg cctccgtgga tgccggcctt gaactcgggt acgaccccat accgcgcgag
     1741 ataccgatcc ttccgctcgt ggatgcgctc ggtcacgagg aagacgcagc gcagacagtt
     1801 gccctggcgc agcccccgct cgaccgtcca ggtcaccacc gcctcgtccc cgacgtactg
     1861 gtagatccgg cctcgcgtct cgagaattgg ctccgcgatg tcgtggaaga agtcgttctt
     1921 gaaatcgttg aagcggaggg ggcccagcgc ggccgcgatc gcggtggagt cgttcaggtc
     1981 gaggaacatg aagatgcgct cctccgcgac gggtcggtga taccgcccca gcaggagact
     2041 caccagggta cccgggccga gcatgcggtt gagctgccgg aagaagttga tgagaaagct
     2101 cgccagggtg aggatcgcca gcgccatcag gaaccgaccg cccagcacga agtcccaata
     2161 ttgctcggac gtgtagtacg cgagcaggcc cccgtgctcg gagagcccga gcaggctcat
     2221 ggtcacgacg tggatgacga cggcgcacag ggtgacgtag aagaaggtcc ggacgaggag
     2281 gagaccgcca acggtcagcg agcgcacgtg gcgcgggatg aggcgcagct cgaaccacgc
     2341 gctggcgagc ccgatggccg ccccggtggc ggtcgcggtg agctcacgag cgaggtgcgt
     2401 cggcacccgc cggacgaccc aatgcacgag cagggccgct ccgatccagc cgcacgtgat
     2461 ccagagagcg gtccgcgcgg tgtggagcgt tcgtggggag accaccggtc agatctcgat
     2521 ctcgatccag gcgcggcgcg ggagccggcc ggtgagccgg gagaacaggt cgcccacccg
     2581 cgccggccag ccgtatttcg cccgcatcgc cgcgtgcgct cgctcgatga cgcggggctc
     2641 ggcgacgacg cgcgcctcgg cgtcccacca ctccccgcgc acgttgccgc gcgcgtcgga
     2701 gggcgcgatc cgcgcccggg gcgagtggcg caggcgcttg accttgccgg catcgccggc
     2761 gctgaacacg tagagcttgc cgtccatcgc cgcgaaccag accggagtcc tgacctcggc
     2821 tccgctgcgt cggaaggtcg cgagcgcgac gtagcgccgg cggtcgagcg cggcgatgtc
     2881 cttcatgcgg gtgcgatcct aggccagccc gatcgatcgg gcgaccgcgt ccaggt
//
LOCUS       DATMCV010000785         5164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_309007, whole genome shotgun sequence.
ACCESSION   DATMCV010000785 DATMCV010000000
VERSION     DATMCV010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309007"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VNU03_17990"
     CDS             <1..741
                     /locus_tag="VNU03_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWP76416.1"
                     /translation="DHLRRWSAQDGVTTFRKPCNKSNGLTWDQQGRLLACEHASSQVS
                     RTEPDGRITPLATHYQGKQINSPNDIVCGADGAIYFSDPPYGRAEFYGVARPQELAFQ
                     GVFRVGPDPKSPFLVADDFDRPNGLCFSLDGRRLFVNDTARQHIRVFDVKTDGTLSGG
                     KVWAETTGEGKGAPDGMKIDSRGNVYCCGPGGIHVLSPEAVSLGVIHVPEYVANFAWG
                     DADCRSLYITASTSVYRIRTVTPGLPAW"
     gene            762..1952
                     /locus_tag="VNU03_17995"
     CDS             762..1952
                     /locus_tag="VNU03_17995"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76417.1"
                     /translation="MMLLRVLTMLLALALAVPMAAEAQKPIKVGMPIPLSGPPALFGD
                     PASKGAQMYVDELNAKGGVLGRKVELIIRDSKADANEAVRVAREMILKDNVDFLVGTL
                     TSAEGPAVSVVAKENKIVFIAPIPKTDQLTAADKLHPYVFRIAANTTMEGRSAAEIVA
                     KWPVTKVATIAFDYAYGQDVTKAFVEHMKKIKPSVQIVDQQWPKLGEQDYNPFINAQM
                     AKKPEAIVSSIWGGFFVTYAKQGKALGMFDAVKYNFIGLGEAATPETTKSMGADYPVG
                     IWGNSYDAFYWGETAAHREYTERISKYLKDEYPSSWAIQGYTGMQFLIEGIKKAGSTD
                     SDKVSKALLGLTIDTPIGKQTIREKDHQANRGQLYGKTVKDPRFPFAIMKPIEYVDPT
                     KFMD"
     gene            2081..2932
                     /locus_tag="VNU03_18000"
     CDS             2081..2932
                     /locus_tag="VNU03_18000"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76418.1"
                     /translation="MSGLTAAMFLFLIASGLSLIFGVLRVLNFAHGTFYMLGAYSAFQ
                     IVQWIGLDGGRFWLAALGAALAVAALGGIVERVLFRHLYGKEELYQLLFTYALVLILS
                     DVAKLIWGTQQKSVSRPPELSGAFHVFGATVPHYNLFILLLGPTIALAFWFVLQRTRA
                     GRFIRAAALDRETLGALGVNVDGLYTWVFVIASFLGGLGGALIAPMRATTPGMDTEVI
                     VEAFVVVVIGGLGSFWGTFLGALIYGQVLSFGILFFPRFSIFSVFALMAAVLIIRPWG
                     LLGRPLK"
     gene            3063..4013
                     /locus_tag="VNU03_18005"
     CDS             3063..4013
                     /locus_tag="VNU03_18005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76419.1"
                     /translation="MLLAAFFVPALGSRFYTFLVNDMVIWALFATSLNLLVGYTGLVS
                     FGHAAYFGIGAYATGLLMKQAGVSFLIAFPAAGVVAGLCALVFGFFCVRLTRIYFAML
                     TLAFAQIVWAICFKWNEVTGGEQGMPEIPYPGFDWLDRIVSSMPFLGSYRTSDYYYFT
                     CLVLVALCLWLLKRIVASPFGRMLTTIRENAERAEFIGVNVRRYELAAFVLAGVFAGL
                     AGGLFGIFNRGVFPDFAYWTKSSEVLIMTLLGGMGTFYGPAVGAVALIWLNQQIVSYT
                     QYWPLILGTILVVLLFVFPGGLAGAVIALWHRLARRPAGA"
     gene            4006..4743
                     /locus_tag="VNU03_18010"
     CDS             4006..4743
                     /locus_tag="VNU03_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76420.1"
                     /translation="MLEVRGLSKNFGGFMAVGGVSFTVPRGSISAIIGPNGAGKTTLF
                     NLITGHIEPDEGRVVFKERDVTGIAPHDLCRLGMGRSFQRTNIFPRLTVYENIQAAYV
                     SHRGRGFNMFTPVERLYRPETESLLQAVGLLEKASETSGFLSHGNQKQLELGITLALE
                     PEILLLDEPTAGMSATETRESIRLIERIAADRGLTLLFTEHDMEVVFSIAHRITVLHQ
                     GRVIADGTPAEVRRDPEVRRVYLGESR"
     gene            4740..>5164
                     /locus_tag="VNU03_18015"
     CDS             4740..>5164
                     /locus_tag="VNU03_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP76421.1"
                     /translation="MTAETGAVPAVPILTVPILTVDDVYTAYGLSQVLFGVSLEVRRG
                     ECVCLLGRNGVGKTTTMRSIIGLTPPTRGRVMWKGRDVAGRPAYEIARAGIGFIPEDR
                     RIFADLTVWENLDVATRRSREGGFTMERVFDLFPKLREL"
BASE COUNT          783 a         1814 c         1675 g          892 t
ORIGIN      
        1 gaccacctcc gccgctggtc cgcccaggac ggggtcacca cgttccgcaa gccgtgcaac
       61 aagtccaacg ggctcacgtg ggaccagcaa ggccggctgc tcgcctgcga gcacgcctcc
      121 agccaggtgt cgcggaccga gcccgacggc cggatcacgc cgctcgccac ccactaccag
      181 ggcaaacaga tcaacagccc gaacgacatc gtctgcgggg ccgacggggc catctacttc
      241 agtgatccgc cgtacggccg cgccgagttc tacggtgtcg cgcggccgca ggagctggcg
      301 tttcagggcg tgttccgggt cggcccggat ccgaagagcc cgttcctggt ggccgacgac
      361 ttcgaccggc ccaacgggct ctgcttctcg ctcgacggcc ggcggctctt cgtcaacgac
      421 accgcccgcc agcacatccg ggtcttcgac gtgaagaccg acggcacgct ctccggcggc
      481 aaggtctggg cggagaccac cggcgagggc aagggcgcgc ccgacggcat gaagatcgac
      541 tcccggggca atgtgtactg ctgcggcccc ggcggcatcc acgtcctgag cccggaggcg
      601 gtgagcctcg gggtgatcca tgtgcccgag tacgtggcca acttcgcctg gggcgacgcg
      661 gattgccgca gcctctacat caccgcgtcc acctcggtgt accgcatccg gaccgtcacg
      721 ccgggcctgc ccgcgtggtg aacggccaaa ggaggctctc aatgatgttg ctgcgcgtac
      781 tgacgatgct gctcgcgctg gccctcgccg tgccgatggc cgcggaggcc cagaagccga
      841 tcaaggtcgg gatgccgatt ccgctgtccg gcccgccggc gctcttcggc gaccccgcct
      901 ccaagggcgc ccagatgtat gtcgacgagc tgaacgccaa gggtggcgtg ctcggccgca
      961 aggtcgagct gatcatccgc gactccaagg ccgacgccaa cgaggcggtg cgggtggcgc
     1021 gcgagatgat cctcaaggac aacgtggact tcctggtggg gacgctcacc tccgcggagg
     1081 gcccggcggt ctcggtggtg gcgaaggaga acaagatcgt cttcatcgcg ccgatcccga
     1141 agaccgacca gctcaccgcg gccgacaagc tgcaccccta cgtgttccgc atcgcggcca
     1201 acaccaccat ggagggccgc agcgcggccg agatcgtggc caagtggccg gtgaccaagg
     1261 tcgccaccat cgccttcgac tacgcctacg gccaggacgt gaccaaggcg ttcgtcgagc
     1321 acatgaagaa gatcaagccg agcgtgcaga tcgtggacca gcagtggccg aagctcggcg
     1381 agcaggacta caacccgttc atcaacgccc agatggccaa gaagccggag gccatcgtgt
     1441 cgtcgatctg gggcggcttc ttcgtcacct acgccaagca gggcaaggcc ctcggcatgt
     1501 tcgacgcggt gaagtacaac ttcatcggcc tgggcgaggc ggcgaccccg gagaccacca
     1561 agtcgatggg cgcggactac ccggtgggca tctggggcaa ctcctacgac gcgttctact
     1621 ggggcgagac cgcggcccac cgcgagtata ccgagcggat ctccaagtac ctgaaggacg
     1681 agtacccgtc gtcgtgggcc attcagggct acaccggcat gcagttcctg atcgagggga
     1741 tcaagaaggc gggcagcacc gactcggaca aggtctcgaa ggccctgctg ggtctcacca
     1801 tcgatacgcc gatcggcaag cagactattc gagaaaaaga tcatcaggcg aaccgcggcc
     1861 aactctacgg caagacggtg aaggatccgc ggtttccgtt cgcgatcatg aagccgatcg
     1921 agtacgtgga ccctaccaag ttcatggatt agcgtgctgg cccgccgccc tccggctacc
     1981 cgcccccgac cataacctcg atatgctccc cgctcgatag agtcagccgc tagatcctcg
     2041 gccccggatg ccggatccgt cgtttgtttt tgcgcagagc ttgagcggcc tgaccgccgc
     2101 gatgttcctt ttcctgatcg cgtcggggct ctcgctcatc ttcggggtgc tgcgggtcct
     2161 caacttcgcc cacgggacct tctacatgct gggggcgtac tccgccttcc agatcgtgca
     2221 gtggatcggg ctcgacgggg gccgcttctg gctcgccgcc ctcggcgccg ccctcgcggt
     2281 cgccgccctg ggcgggatcg tggagcgcgt cctcttccgg cacctctatg gcaaggaaga
     2341 gctctaccag ctcctcttca cctacgccct ggtgctgatc ctgagcgacg tggccaagct
     2401 catctggggt acccagcaga agtcggtgag ccggccgccc gagctgagcg gggccttcca
     2461 cgtcttcggc gccaccgtgc cccactacaa cctcttcatc ctgctcctgg gcccgacgat
     2521 cgcgctcgcc ttctggttcg tgctgcagcg cacccgggcg ggccggttca tccgggccgc
     2581 cgcgctcgat cgcgagacgc tcggcgccct cggggtcaac gtcgacggcc tctacacctg
     2641 ggtcttcgtg atcgcctcgt tcctcggcgg gctcggcggc gccctgatcg cgccgatgcg
     2701 ggccaccact cccgggatgg acaccgaggt gatcgtggag gctttcgtgg tggtggtgat
     2761 cggcggcctc ggctccttct ggggcacctt cctcggggcg ctgatctacg ggcaggtgct
     2821 ctccttcggc atcctcttct tcccgcgctt ctcgatcttc tcggtcttcg ccctcatggc
     2881 cgcggtcctg atcatccgcc cctggggcct cctgggccga cccctgaagt agcgagcgga
     2941 ccaatgatct cgacaacgcg attgatcaat gtggtggggg ggcttggggg aggagcggcg
     3001 ccgctccccc ccaattcaaa ataatgccgt cacggtggcc gcgaatccca tgggccctcc
     3061 tcgtgctgct cgccgcgttc ttcgtgccgg cgctgggctc gcgcttctac acgttcctgg
     3121 tcaacgacat ggtcatctgg gccctcttcg ccaccagcct gaacctcctg gtgggctaca
     3181 ccgggctcgt gtcgttcggc cacgccgcct acttcgggat cggggcctac gccaccgggc
     3241 tcctcatgaa gcaggcaggc gtctcgttcc tgatcgcgtt cccggccgcc ggggtggtcg
     3301 ccggtctctg cgccctcgtc ttcggcttct tctgcgtgcg cctcacccgc atctacttcg
     3361 cgatgctgac cctcgccttc gcccagatcg tgtgggccat ctgcttcaag tggaacgagg
     3421 tgaccggcgg cgagcagggc atgcccgaga tcccctaccc gggcttcgac tggctcgacc
     3481 ggatcgtctc gtcgatgccg ttcctgggga gctaccgcac gtcggactac tactacttca
     3541 cctgcctggt cctggtcgcg ctctgcctgt ggctcctgaa gcgcatcgtg gcctcgccct
     3601 tcggccgcat gctcaccacc atccgggaga acgcggagcg cgccgagttc atcggggtga
     3661 acgtgcgtcg ctacgagctg gccgccttcg tcctcgcggg cgtgttcgcc ggtctggccg
     3721 gggggctctt cgggatcttc aaccgcgggg tcttcccgga cttcgcgtac tggaccaagt
     3781 cgtccgaggt gctgatcatg accctcctgg gcggcatggg caccttctac ggcccggcgg
     3841 tgggcgcggt ggccctcatc tggctcaacc agcagatcgt ctcctatacc cagtactggc
     3901 cgctgatcct gggcaccatc ctggtcgtcc tcctgttcgt ctttcccggc gggctggccg
     3961 gcgcggtgat cgcgctctgg caccgcctcg cccggaggcc cgccggtgct tgaggtccgc
     4021 gggctctcca agaacttcgg cggcttcatg gcggtgggcg gggtgtcgtt cacggtgccg
     4081 cgcgggtcga tcagcgcgat catcgggccc aacggggcgg gcaagaccac gctcttcaac
     4141 ctgatcaccg ggcacatcga gcccgacgag gggcgcgtgg tgttcaagga gcgcgacgtc
     4201 accggcatcg ccccccacga tctctgccgc ctcgggatgg gccgatcgtt ccagcggacc
     4261 aacatcttcc cgcggctcac cgtgtacgag aacatccagg ccgcctacgt gtcccaccgc
     4321 ggccgcgggt tcaacatgtt caccccggtg gagcggctct accgcccgga gacggagagc
     4381 ctcctgcagg cggtgggcct gctggagaag gcgagcgaga cgtcgggctt cctctcccac
     4441 ggcaaccaga agcagctgga gctcggcatc accctcgcgc tcgagcccga gatcctgctg
     4501 ctcgacgagc ccaccgccgg gatgtccgcg accgagaccc gcgagtcgat ccggctcatc
     4561 gagcgcatcg cggccgaccg gggcctcacc ctgctcttca ccgagcacga catggaggtc
     4621 gtcttctcca tcgcgcaccg catcacggtg ctgcaccagg gccgggtgat cgcggacgga
     4681 acgcccgccg aggtgcgacg cgatcccgag gtgagacgcg tctacctggg agagagccgg
     4741 tgaccgcgga gaccggcgcc gtccccgcgg tccccatcct gacggtgccc atcctgaccg
     4801 tggacgacgt ctacaccgcc tacgggctgt cgcaggtcct cttcggggtc tcgctcgagg
     4861 tgcggcgggg cgagtgcgtg tgcctgctcg ggcgcaacgg ggtcggcaag accaccacca
     4921 tgcgcagcat catcggcctc acccctccga cccgcgggcg ggtgatgtgg aaggggcgcg
     4981 acgtcgcggg gcgccccgcc tacgagatcg cgcgggccgg catcgggttc atcccggaag
     5041 accggcgcat cttcgcggat ctcacggtgt gggagaacct cgacgttgcc acgcgccgga
     5101 gccgcgaggg cggcttcacg atggagcggg tcttcgatct cttccccaag ctgcgggagc
     5161 tgat
//
LOCUS       DATMCV010000786         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_774772, whole genome shotgun sequence.
ACCESSION   DATMCV010000786 DATMCV010000000
VERSION     DATMCV010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774772"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1530)
                     /locus_tag="VNU03_18020"
     CDS             complement(187..1530)
                     /locus_tag="VNU03_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWP76422.1"
                     /translation="MTKSELMRKVHATIDRRGDEIIALGEAIRRQPELGFKEFKTAKL
                     VAQTFERIGLTPRTGLALTGVRADVIGRAGDGPTLALFGELDGLVVVGHPVADPETGA
                     AHACGHNAQVAGMLGAAMGLLEAGAFDHLAGRVAFVGVPAEEYGDVEWRVAQRDAGRL
                     EFMGGKPEMLRLGHLDDVAMGMMIHATPRREEGKLAVPMSNNGCVVKTVRYVGRASHA
                     GGAPHMGINALYAAQIGLMAINALRETFRDEDTIRVHPIITHGGSQVNVIPGEVRLET
                     FVRGKSLEAIADADRKVDRALRAGALAMGASVEIETLPGYMPLRCDPLMVERFRAIAA
                     DLVGADNVRTILHRTGSTDMGDLGQVMPIIHPYVGGAKGTPHGADYEIVDPALTYLTN
                     AKALASMVIDLLADNATVGRQVVAQAKPPLTRDGYLAFQRKMSRRETYDGATTDR"
     gene            complement(1680..>2057)
                     /locus_tag="VNU03_18025"
     CDS             complement(1680..>2057)
                     /locus_tag="VNU03_18025"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HWP76423.1"
                     /translation="YARPHPQYVYAGMSLQRRLIGDLYKTVRELAGGAFQHLPSSERS
                     FESPETRAATERYYQSIGTPARDRVKLLKLIWDFIGTEYGGRQLQYEMFYSAAQPVVN
                     RRMFRSYDWAAARAMVDRLLSEY"
BASE COUNT          276 a          732 c          730 g          319 t
ORIGIN      
        1 cgtcctcacc tcgcgtcagc agttcccaat ctgttagcgg ccgctgtagg cgatccccgg
       61 agggaacggg tcacccggcc tacccccgcc gccccgcccc cgcccgctcc ctccctaccc
      121 gcttggttct catcccggct cacactcgtt ccagcgaagc tgggcccgct ggtcggtggg
      181 cggggctcag cggtcggtgg tggcgccgtc gtaggtttcg cggcggctca tcttgcgctg
      241 gaacgcgagg tatccgtcgc gggtgagcgg gggcttggct tgggcgacga cctggcggcc
      301 gacggtggcg ttgtcggcga ggaggtcgat gaccatggag gcgagggcct tggcgttagt
      361 cagataagtc agcgcggggt cgacgatctc gtagtcggcg ccgtgcgggg tgcccttggc
      421 gccgcccacg tagggatgaa tgatcggcat gacctggccg aggtcgccca tgtcggtcga
      481 gccggtgcgg tgcaggatgg tgcgcacgtt gtcggcgccg accaggtccg ccgcgatggc
      541 ccggaagcgc tccaccatca gcgggtcgca gcggagcggc atgtagccgg gcagggtctc
      601 gatctccacc gaggcgccca tcgcgagggc ccccgcccgc agggcgcgat ccaccttgcg
      661 gtcggcgtcc gcgatggcct cgagcgactt gccgcgcacg aaggtctcga ggcgcacctc
      721 gcccgggatc acgttcacct gcgagccgcc gtgggtgatg atcgggtgca cccggatggt
      781 gtcctcgtcg cggaaggttt cccgcagcgc gttgatggcc atgagcccga tctgggccgc
      841 gtagagcgcg ttgatcccca tgtgcggggc gccgcccgcg tgggaggcgc ggcccacgta
      901 gcgcaccgtc ttcaccacgc agccgttgtt ggacatgggc accgccagct tgccctcctc
      961 gcgccgcggg gtggcgtgga tcatcatgcc catggcgacg tcgtcgaggt ggccgagccg
     1021 cagcatctcc ggcttgccgc ccatgaactc gagcctcccc gcgtcccgct gcgccacccg
     1081 ccactccacg tcaccgtact cctccgccgg cacgcccacg aaggcaaccc gcccggccag
     1141 gtgatcgaag gcgcccgcct cgagcaggcc catcgccgcg cccagcatgc ccgcgacctg
     1201 tgcgttgtgc ccgcaggcgt gcgcggcgcc ggtctccgga tccgccaccg ggtggccgac
     1261 caccacgagc ccgtcgagct cgccgaagag cgcgagggtg gggccgtcgc ccgcgcgccc
     1321 gatcacgtcg gcgcgaacgc cggtgagggc cagaccggtg cgcggggtga gcccgatgcg
     1381 ctcgaaggtc tgggcgacca gcttggcggt cttgaactcc ttgaagccga gctccggttg
     1441 gcggcggatc gcctcgccca gcgcgatgat ctcgtcgccc cgccggtcga tcgtcgcgtg
     1501 gaccttccgc atcagctcac tcttggtcat gggatgctcc tcctcgtagg aggccgccga
     1561 gaaaagtcca gctgcgaggc ggcgcccgaa ggcggcacgc gaagcgtact ccctgtacgc
     1621 tgagcgtgcc gccgagggcg ccaacgaagc agatgggcct ttatccgccg ccggcgcgct
     1681 cagtactccg agaggaggcg gtcgaccatc gcccgggccg cggcccagtc gtaggagcgg
     1741 aacatgcggc ggttcaccac cggctgggcc gcggagtaga acatctcgta ctggagctgg
     1801 cgccctccgt actccgtgcc gatgaagtcc cagatcagct tgagcagctt cacccggtcc
     1861 cgcgcgggcg tgccgatcga ctggtagtag cgctcggtgg ccgcgcgcgt ctccggcgac
     1921 tcgaagctcc gctccgacga gggcaggtgc tggaaggcgc cgccggccag ctcgcgcacc
     1981 gtcttgtaga ggtcgccgat cagccgccgc tgcaggctca tcccggcgta gacgtactgc
     2041 gggtgcgggc gcgcgta
//
LOCUS       DATMCV010000787         4319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_108783, whole genome shotgun sequence.
ACCESSION   DATMCV010000787 DATMCV010000000
VERSION     DATMCV010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108783"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VNU03_18030"
     CDS             <1..233
                     /locus_tag="VNU03_18030"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWP76424.1"
                     /translation="LDEDDYEVQLFEDALVVEGRRWLPPRGAGGLYHTAGVRQGPFRL
                     EVRLPAPVDGERIEAHYDRGLLRISLPKQRRP"
     gene            233..2650
                     /gene="lon"
                     /locus_tag="VNU03_18035"
     CDS             233..2650
                     /gene="lon"
                     /locus_tag="VNU03_18035"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWP76425.1"
                     /translation="MADEPTGDFTIPDALPVLPLRELVLFPLTAVPLAVGQARSLRLV
                     DDVMRSNRLLALVAQRDPKPEMAGPDDLYGVGTVGVIHQLARAPDGTVRLMIQGLERI
                     RIVGWVGTEPYLVARIEVAKEDAASDTEAGALRVALLDIFRRLVSASPDRPEELATTA
                     ESIVDPRHVAYFIATVIPLDVAVRQELLELEPLPAKLRRLVDVLQRELAVRELGRKIT
                     TDTEERLSKNQRDFYLREQLRSIQKELGEEGARDGGAADLRRRIEEAGLPEEARREAT
                     RELTRLAGLSPSSPEHAMIGTYLEWLASLPWSRLAGGPIDIQRAREVLNQDHYDLEKI
                     KDRILEYLAVKKLRQERAAPAKAPREAGPGPAAEPAREPILCFVGPPGVGKTSLGQSI
                     ARALGRRFARMSLGGVRDEAEIRGHRRTYIGALPGRIIQGLRRAETRDPVFMLDEIDK
                     IGSDWRGDPSSALLEVLDPAQNHTFVDNYLGVSFDLSQVFFIATANTLETIPGALRDR
                     LEILTLSGYTDEEKIGIATAFLIPKQLAAHGLSPEELAFDREAIRQIVRGYTREAGVR
                     NLEREIAAVMRKVARRIAEGQREPVRIASDRVSEYLGRPRFVDEVAERTTRPGVATGL
                     AWTPVGGDVLFVEVTMMPSNEERLVLTGMLGDVMRESAQAAVSYVWSNASVLDIDPEL
                     FEGKTIHVHVPAGAIPKDGPSAGVTIVTALASLATRRPVRSDVAMTGEITLRGKVLPV
                     GGIKEKVLAAHRAGIRTVILPRRNERDLDDVPEELRRQLAFALVDDADEVLRHALTPA
                     VVGAAAS"
     gene            complement(2655..>4319)
                     /locus_tag="VNU03_18040"
     CDS             complement(2655..>4319)
                     /locus_tag="VNU03_18040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWP76426.1"
                     /translation="LGIEGEAGVLVAGSRRDDFPSNTEVLLLRVAANQAEMGLRAARL
                     LGEHRRERHLVDEDRRALAALVEHSSDFIALATLEGRVFFLNPAGQRLVGIRAEEQVR
                     ATRISDYITEDDREWFESSVMPTVLRAGSWQGEARFRHFHSGAAIPVLQDIFIIKDWS
                     RDRPLSVGTIGRGITDRMRAEQSVRESERRLRLQAEAIPHQVWTRLPDGTVDYCNRRF
                     IDYTGLTLEAMQQFGLTAVVHPGDVERVASAWKEARARLAPYEVEKRLRGVDGRYRRF
                     LSRAVPVCDEQGRLLHWIGTYTDVEDRKQAEDALRDAQAALAQVSRLTTMGELAATLA
                     HEMNQPLTAVVANGGACLRWLDRAQPDIAEAMGAVRRMIRDANRAADVVGHARALVNR
                     STGEKTGLDLDEVIREITTLTQGELLRHRVVLREVLDRNVPPVLGDRIQLQQVLLNLV
                     MNAIEAMADIEECRELTIRSAPQEFDGGPGALVAVHDTGAGVAPENLDRLFDAFYTTK
                     PDGLGLGLSISRTIIEGHGGRLWATANAGPGTTFQFALPASGKKPS"
BASE COUNT          649 a         1546 c         1485 g          639 t
ORIGIN      
        1 gcctggacga ggacgactac gaggtgcagc tcttcgagga cgcgctggta gtcgaggggc
       61 gccggtggct gcccccccgt ggcgccggcg gtctctacca cacggccggc gtccgccagg
      121 ggcccttccg cctcgaggtg cgactgccgg caccagtgga cggcgagcgc atcgaggccc
      181 actacgaccg cgggctgctc cgcatctccc tgccgaagca gaggagaccc tgatggcgga
      241 cgagcccacc ggcgacttca cgatccccga tgcgctcccg gtcctcccgc tccgggagct
      301 cgtgctgttc ccgctcaccg cggtcccgct ggcggtgggt caggctcgct ccctccgcct
      361 ggtggacgac gtgatgcgga gcaaccgctt gctggccctg gtggcccagc gcgatcccaa
      421 gccggagatg gcgggccccg acgacctcta cggagtcggc accgtcggag tgatccacca
      481 gctcgcccgg gcgcccgacg gcacggtgcg tctgatgatc cagggtctcg agcgcatccg
      541 catcgtgggc tgggtgggaa ccgagcccta cctggtggcg cggatcgagg tggccaagga
      601 ggacgcggcc agcgacaccg aggcgggcgc gctgcgcgtg gccctgctcg acatctttcg
      661 ccgtctcgtg agcgcctcgc ccgaccggcc ggaagagctg gccaccacgg cggagagcat
      721 cgtcgatccc cgccatgtcg catacttcat cgccacggtg atcccgctcg acgtggccgt
      781 ccggcaagag ctgctcgagc tggagccact cccggccaaa ctccggcgcc tagtggacgt
      841 gctccagcgc gagctggccg tgcgcgagct ggggcggaag atcaccaccg acaccgagga
      901 gcggctctcc aagaatcagc gcgacttcta ccttcgcgag cagctccgct ccatccagaa
      961 ggagctgggt gaagagggag cccgtgacgg cggcgcggcc gaccttcgcc gccggatcga
     1021 ggaggccggg ctgccggagg aggccaggcg cgaggcgacg cgggagctga cccggctggc
     1081 gggcctgtca ccctcttcgc ccgagcacgc gatgatcggg acctacctcg agtggctggc
     1141 gagcctgccg tggagccggc tcgcgggcgg ccccatcgac atccagcgag cccgggaggt
     1201 gctgaaccag gatcactacg acctggagaa gatcaaggac cgcattctcg aatacctggc
     1261 ggtcaagaag ctccggcagg agcgggccgc gccggcgaag gcgccccggg aagcggggcc
     1321 ggggccagcc gccgagccgg cgcgcgagcc catcctctgc ttcgtcggac cccccggcgt
     1381 cggcaagacc agcctcggcc agtccatcgc ccgagcgctg ggccgccgct tcgcgcgcat
     1441 gtcgctcggc ggggtgcgag acgaggcgga gatccggggt caccgacgca cctacatcgg
     1501 ggccctgccc ggccggatca tccaggggct caggcgcgcg gagacgcgcg atccggtctt
     1561 catgctggac gagatcgaca agatcggctc cgactggcgg ggcgacccct cgtccgcact
     1621 cctggaggtg ctcgatccgg ctcagaacca caccttcgtg gacaactacc tcggcgtgtc
     1681 gttcgacctc tcacaggtct tcttcatcgc gaccgccaac accctggaga ccatccccgg
     1741 cgccctgcgc gaccggctgg agatcctgac cctgtcgggc tacaccgacg aggagaagat
     1801 cgggatcgcc accgcgttcc tgatcccgaa gcagctcgcc gcgcacggcc tgtcgccgga
     1861 ggagctggcc ttcgaccggg aagcgatccg tcagatcgtg cggggctaca cccgggaggc
     1921 cggggtgcga aacctcgagc gggagatcgc ggcggtgatg cgaaaagtcg cccggcgcat
     1981 cgccgagggc cagcgcgagc cggtccggat cgcttccgac cgggtcagcg agtacctcgg
     2041 ccgcccgcgc ttcgtcgacg aggtggccga gcggaccacg cggcccgggg tggccaccgg
     2101 gctcgcctgg acgccggtgg gcggcgacgt gctattcgtc gaggtcacca tgatgccgag
     2161 caacgaggag cggctggtgc tcaccggcat gctcggcgac gtcatgcgcg aaagcgccca
     2221 ggccgcggtg tcctatgtct ggtcgaacgc gtccgtgctc gacatcgatc cggagctgtt
     2281 cgagggcaag accatccacg tgcacgtgcc cgccggcgcc attcccaagg acgggccctc
     2341 cgccggggtg accatcgtga ccgcgctggc ctcgctcgct actcgccggc cggtgcgcag
     2401 cgacgtggcg atgaccgggg agatcacgct gcggggcaag gtactgcccg tcggcgggat
     2461 caaggagaag gtcctggccg cccatcgggc cggcatccgc accgtgatcc tgccccggag
     2521 aaacgagcgc gacctcgacg acgtgccgga ggagcttcgg cgccagctcg ccttcgccct
     2581 ggtcgacgac gcggatgagg tcctgagaca tgccctcacc cccgccgtcg tcggcgcggc
     2641 ggcctcctag acgctcaaga cggtttcttg cccgaggcgg ggagggcgaa ctggaaggtc
     2701 gttcccgggc cggcattggc ggtcgcccac agccggccgc cgtgtccctc gatgatggtc
     2761 cggctgatcg acagtcccag cccgagaccg tcgggtttcg tggtgtagaa cgcgtcgaag
     2821 agccggtcga gattttcagg cgcgaccccc gctcccgtgt cgtggacggc caccagggcg
     2881 ccggggcctc cgtcgaactc ctggggcgcc gatcggatcg tgagctcccg gcactcctcg
     2941 atgtcggcca tcgcctcgat cgcgttcatg accaggttga gtagcacctg ctggagctgg
     3001 atccggtcac ccagcaccgg cggcacattc cggtccagca cctcgcggag cactacgcga
     3061 tgccgcagga gctcgccctg ggtcagggtg gtgatctcgc ggatcacttc gtcgaggtcg
     3121 agcccggttt tctctccggt cgacctgttc accagcgccc gcgcgtgccc gaccacgtcg
     3181 gcggcccgat tcgcgtcgcg gatcatccgc cggaccgcgc ccatcgcctc cgcgatatcc
     3241 ggctgggcgc gatccagcca gcgcaggcag gcgccgccgt tggcgacgac ggcggtcagg
     3301 ggctggttca tctcgtgggc cagagtcgcc gccagctccc ccatcgtggt caagcgcgag
     3361 acttgcgcga gcgcggcctg cgcgtcgcgc agggcgtcct ccgcctgctt gcgatcctcg
     3421 acgtcggtat acgtcccgat ccagtggagc agccggccct gttcgtcgca caccggaacc
     3481 gcgcgcgaca ggaagcgtcg gtaccgaccg tcgactccgc gcaggcgctt ttccacctcg
     3541 tacggggcca gccgggccct cgcctccttc cacgcgctcg cgacgcgttc gacatcaccg
     3601 ggatgcacga ccgcggtcaa gccgaattgt tgcatcgcct cgagggtgag ccccgtgtag
     3661 tcgatgaagc ggcggttgca gtagtccacg gtgccgtccg ggagcctggt ccatacctga
     3721 tgagggatcg cttccgcctg caggcgaagc cgacgctcac tctcccgaac gctctgctcg
     3781 gcccgcatgc gatccgtgat gccgcggccg atcgtgccga ccgagagggg acgatccctg
     3841 ctccaatcct tgatgatgaa gatgtcctgc agcaccggaa tcgcggcgcc gctgtgaaag
     3901 tgccggaatc gcgcctcccc ttgccagctt cccgctctca gcaccgtcgg catgacgctc
     3961 gactcgaacc actcgcgatc atcctcggtg atgtagtccg agatccgggt ggcgcgcacc
     4021 tgctcttccg cccggatccc gaccaggcgc tgccccgccg ggttgaggaa gaagacccgg
     4081 ccctcgagag tcgcgagggc gatgaagtcg gagctgtgct cgaccagcgc ggcgagcgcc
     4141 cggcggtcct cgtcgacgag gtggcgctcc cgccggtgct cgcccaggag ccgagctgcg
     4201 cgcaggccca tttcagcctg gttggcggcc acccgcaaga ggagcacctc cgtgttcgag
     4261 gggaaatcgt cgcgtcggga ccctgcgacc agcacgccag cctcgccctc gattcccag
//
LOCUS       DATMCV010000788         5799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_108829, whole genome shotgun sequence.
ACCESSION   DATMCV010000788 DATMCV010000000
VERSION     DATMCV010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108829"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VNU03_18045"
     CDS             <1..851
                     /locus_tag="VNU03_18045"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWP76427.1"
                     /translation="AVNTALMVGCLLLVIGFRSSAALGGAYGVAVTGTMATTTVLFYA
                     IARYRWRWSRWSAGVLTVVFLAVDLAFLAANVGKIAAGAWVPLVVAGAIVLVMTTWHR
                     GSDLVMSTLSRLSMPLDVFLRTAEEQKPPRVPGTAVFLTPTLQGAPLSLVRHFRHNKV
                     LHEEVILVSIVTEEVPEVEEARRMESEPLGFGFWRVRAHYGFLERPDVQWVVARCCDR
                     GMSAAPEETTFFLGRASLLPVGSAPMPRWRKRLFALMSWNASSATDFFQVPPDQVLEV
                     GARIEF"
     gene            complement(867..1433)
                     /locus_tag="VNU03_18050"
     CDS             complement(867..1433)
                     /locus_tag="VNU03_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWP76428.1"
                     /translation="MVIGPHVGQSRVARLILPISLHDHVQGAVDAPVTLVEYGDYECP
                     FYGRAHPIIKSVQHRLGRRLRFVYRHFPLAEMHPRAFRAAEAAEAAGARRKFWPMHDM
                     QFERQQALEDTDLMSYAEYLRLDLPRFVADLADGTYEMKVRSDFMSGVRSGVNGTPTF
                     FINGVRHDGPWDADTLTEACLATLAAHV"
     gene            complement(1450..1811)
                     /locus_tag="VNU03_18055"
                     /pseudo
     CDS             complement(1450..1811)
                     /locus_tag="VNU03_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            2314..2991
                     /locus_tag="VNU03_18060"
     CDS             2314..2991
                     /locus_tag="VNU03_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWP76429.1"
                     /translation="MATMTATQTRTDEEIQRDVLAELRWDARVRPNEIGVVVKDGVVA
                     LTGWVDSYMKKWSADEAAHRVKGVKAVANDIEVRLPGDQQRIDSDIAAEVVRALQSDA
                     ALRQEKVEVTVSKGWVTLEGEVEWKYQKEDAERVVRRILGVKGVSNYLVVKPRVTPGE
                     LKQKIEQALIRDAETDAERITVEVQGAKVILKGAVRSWAERQEAERVAWSAPGVAQVD
                     NRITVAL"
     gene            3278..3676
                     /locus_tag="VNU03_18065"
     CDS             3278..3676
                     /locus_tag="VNU03_18065"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWP76430.1"
                     /translation="MRRLQGQMEQLFTDSVPTWRWPLTGQYPPLNITRSDTGITLEAL
                     CPGVDRGSLDVQVVGDTVTLRGEHKPEPDAPEEHYYRRERPIGSFTRTLGLGEAVNAD
                     QTQATYTGGILRVQLARSPEATPKKIPVKS"
     gene            3704..4120
                     /locus_tag="VNU03_18070"
     CDS             3704..4120
                     /locus_tag="VNU03_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWP76431.1"
                     /translation="MTTTATPKDQRDKTASRAPGPRDPRDTADRAPRAIPPRVDIYET
                     EKIYVLLADMPGVLPDGLDVVAERDELVIRGRVERPARTPDYQEFELANYYRAFTLTD
                     DLDTDGITARLSDGVLRVEIPKSARTVLKKIPVRME"
     gene            4133..4600
                     /locus_tag="VNU03_18075"
     CDS             4133..4600
                     /locus_tag="VNU03_18075"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWP76432.1"
                     /translation="MAILDKVTSLLPWRSERREHPERPAGAMALRDDLDRWLTRLLEE
                     PFSRYGVDELGTAPTAKVHETDDELVVSIEVPGFDRDELDVRLTPEGLTVRGERRAEA
                     DDEYIHRHFILTVPLPPGLDLDRPEARISRGVLTIRFPRTPTRPGARRIPVRA"
     gene            4615..>5799
                     /gene="dnaK"
                     /locus_tag="VNU03_18080"
     CDS             4615..>5799
                     /gene="dnaK"
                     /locus_tag="VNU03_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWP76433.1"
                     /translation="MAKVIGIDLGTTNSVVAVMEGGEPAVISNQEGSRLTPSVVAFTK
                     DGEILVGHVARRQAITNPENTVFSIKRFMGRRYDEVLEEIKKVPYKVVKAANGDVRVE
                     IRGKLYSPPEISAMILRKLKESAEAHLGDKVTQAVITVPAYFNDSQRQATKDAGAIAG
                     LEVLRIINEPTAAALAYGLDKKADEQIAVYDLGGGTFDISILEIGQGVFEVKATNGDT
                     HLGGDDFDQRVIDWIADEFKKENRIDLRQDRMALQRLKEAAEKAKIELSSTLQTEINL
                     PFVTADATGPKHLVMTLTRARLEALVADLVERTIEPCRRAMKDADVTEADIDEVILVG
                     GQTRMPKVQETVKQLFGKEPHKGVNPDEVVAVGAAAQGGVLSGEVKDVLLLDVTPLSL
                     GIE"
BASE COUNT          981 a         1942 c         2033 g          843 t
ORIGIN      
        1 ccgcggtcaa caccgccctg atggtgggct gtctcctcct cgtcatcggc tttcgctcgt
       61 cggccgccct cggcggggct tacggcgtcg cggtcaccgg caccatggcc accaccaccg
      121 tgctcttcta cgcgatcgcc cgctaccgct ggcgttggtc cagatggtcg gcgggcgtcc
      181 tcaccgtcgt gttcctggcg gtggatctcg cgttcctcgc ggcgaacgtc ggaaagatcg
      241 cggccggcgc gtgggtgccc ctggtcgtcg cgggagccat cgtcctcgtg atgaccacgt
      301 ggcaccgcgg cagcgatctc gtgatgagca ccctgtcgcg cctctcgatg ccgctcgacg
      361 tcttcctcag gacggcggag gagcagaagc cgccccgtgt gccgggcacc gccgtcttcc
      421 tcacgccgac cctgcagggg gcacccttga gcctggtgcg tcatttccgg cacaacaagg
      481 tcctgcacga ggaggtcatc ctggtgtcca tcgtgacgga ggaggttccg gaggtcgagg
      541 aggcccggcg gatggagagc gagcccctgg gcttcggatt ctggcgcgtc cgggcccact
      601 acgggttcct ggagcggccc gacgtccagt gggtggtcgc gcgctgctgc gatcgaggca
      661 tgagcgcggc gcccgaagag accacgttct tcctcgggcg cgcgtcgctt ctgccggtgg
      721 ggtcggcgcc gatgccgcgc tggcggaagc gcctgttcgc gctcatgtcg tggaacgcga
      781 gctcggccac cgatttcttc caggtcccgc ccgaccaggt cctggaggtg ggcgctcgga
      841 tcgagttcta gcgtctagga cgcgcttcag acatgggcgg ccaaggtcgc caggcaagcc
      901 tcggtcaagg tgtcggcgtc ccatggaccg tcgtgccgga cgccgttgat gaagaacgtc
      961 ggggtgccgt tcacgccgct acggaccccg ctcatgaaat cgctccgcac cttcatctcg
     1021 taggtgccgt cggccaggtc ggcgacgaat cgcggcagat cgagcctcag gtactcggca
     1081 tagctcatca gatcggtgtc ctcgagcgcc tgctggcgct cgaactgcat gtcgtgcatg
     1141 ggccagaact tccgccgagc ccccgcggct tccgcggcct ccgcggcgcg gaaggcgcga
     1201 ggatgcatct cggcgagcgg gaaatgccgg tagacgaagc gcagccgccg gccgagacga
     1261 tgctgcacgc tcttgatgat cggatgggcc cgtccataga aggggcactc gtagtccccg
     1321 tattcgacga gcgtgaccgg cgcatccacc gccccctgga cgtggtcgtg caggctgatc
     1381 ggcaggatga ggcgggcaac gcgggactgg ccgacgtgcg gtccgatgac catgggtgcc
     1441 tccttcgatt caggcgcgcc gcgacctggt cggaagcgac tcgagcgcct cgaggatgcc
     1501 gtcggcgccc gggttcactc cgatcggcga caggtagctc cacacgatgg tcccttcgcc
     1561 gtcgagcacg aagagcgccc gcgacgcctt cgcgcgcgcg gtacgctccg tagctccggg
     1621 cgaccgctcc cttgggctcg aagtcggcca ggagggggaa atgcaggcgg ctggtcgccg
     1681 cagaccggac tccagtcggc cggatagaac gcgaggatca ccggctgccc gccgaagtcg
     1741 gaaagggcga cggtctggtc gggtgtggag ttgagggtga acccgggctc gctgatgccg
     1801 ggcttcagga tgcccggggc ccggccagcc ggcgcggtcg acgacgtgcc ttgctcacgc
     1861 ttcggtcccc gatcggcctc cgccatgtgg tgggcctcag gcaagaacga cgccacgcgc
     1921 ctcgtcgtgg cgatgggctc cccgagtggc agattctccg aaggtcggag tgggggacgc
     1981 gggggagatc gggtgacctc acacccaagc gatcgagggg gcggtactgc cggagggcgc
     2041 ggccggcccc ggattccgcg ggattggccg cgggtgcgac cgcacccgtc gcccgcgtgt
     2101 ctcccggggt ccggccaccc gcctcaccca cacagacgga tggggagtca cccggctcgt
     2161 cgaggtccgg cggcgcggcc gacgcgatcc ggcgctcgac ctctcggaaa gccaccgctt
     2221 tacacgctgg cgtcggtctt gcttgccgcg tcgaccgcag gacaccgtcg agaacgggcg
     2281 ctgaccgata tgtagcgaga aaggagagcc gccatggcaa ccatgaccgc cactcagacc
     2341 agaaccgacg aggagatcca gcgcgacgtc ctcgccgagc ttcgatggga cgcccgggtg
     2401 aggcccaacg agatcggggt ggtcgtgaag gacggggtgg tcgctctcac cggctgggtc
     2461 gactcctaca tgaagaagtg gtcggcggac gaggcggccc accgggtgaa gggtgtgaag
     2521 gcggtcgcca acgacatcga ggtgcggttg cccggcgacc agcagcggat cgactcagat
     2581 atcgccgcgg aggtggtgcg agccctgcag tccgatgccg ccctacggca ggagaaggtg
     2641 gaggtcacgg tgtcgaaggg ctgggtgacg ctggagggcg aagtcgagtg gaagtaccag
     2701 aaggaggacg ccgagcgcgt ggtgcggcgc atcctgggcg tcaagggtgt gagcaactac
     2761 ctcgtggtga agccgcgcgt cacaccgggc gagctgaagc agaagatcga gcaggccctg
     2821 atccgcgacg cggagaccga cgccgagcgc atcacggtgg aggtgcaggg cgccaaggtt
     2881 atcttgaagg gggcggtgcg gtcgtgggcg gagcggcagg aggcggagcg ggtggcctgg
     2941 tcggcgcccg gggtggccca ggtcgacaac cggatcacgg tcgccctctg agacgctccc
     3001 gggcttcacc cagcgtggtg ggcgggattg cacccaagcg caaacccgtg ccgggcaaag
     3061 ccgcgacgag cttctggatc gcccggtgga gatcgggtgg gtgcatgctc acccaccccg
     3121 cgggcggagg acgcggatcg tcccgggaag tcgccagatg gagtactggc tttcgtcttg
     3181 cagcgatagg cggagacgat cgaggcagga cgctcgacca acgaacaggg gaggccactc
     3241 gatggacacg cgatcactat gggatccctg gcaggagatg cgtcgcttgc agggccagat
     3301 ggagcagctc ttcaccgatt cggttccgac gtggcggtgg ccgctcaccg gccagtatcc
     3361 gccgctcaac atcacacggt ccgacaccgg gatcacgctc gaagcgctct gcccgggcgt
     3421 ggaccgcggg agcctcgacg tccaggtcgt gggtgacacg gtcacgctcc gcggcgagca
     3481 caagccggag ccggacgccc ccgaggagca ctactaccga cgggagcgtc cgatcgggtc
     3541 cttcacccgc accctcggcc tcggcgaggc cgtgaacgcc gatcagaccc aggcgacgta
     3601 caccggcggg attctccgcg tgcagctcgc ccggtcaccc gaagccaccc ccaagaagat
     3661 tccggtcaag agctgaccga ggaggctcga ccccatggca accatgacca cgaccgcgac
     3721 ccccaaggac cagcgcgaca agaccgcctc gcgggcgccc ggcccgcgcg atccgcggga
     3781 caccgccgat cgcgccccgc gcgcgatccc gccccgcgtg gacatctacg agaccgagaa
     3841 gatctacgtg ctcctggccg acatgcccgg ggtgctgccc gatggcctgg atgtggtggc
     3901 cgagcgcgac gagctggtca tccgcggccg ggtcgagcgc ccggccagga cgcccgacta
     3961 tcaggagttc gagctcgcca actactatcg ggccttcacc ctgaccgacg atctcgacac
     4021 cgatggcatc accgcccggc tcagcgacgg cgtgctgcga gtggagatcc ccaagtcggc
     4081 ccgcacggtg ctcaagaaga tcccggtccg gatggagtag gaggagccgg ccatggcaat
     4141 tctcgacaag gtcacctcgc tcctcccgtg gcgctccgag cggagagagc atccggaacg
     4201 gccggcgggc gcgatggccc tccgtgacga tctcgacagg tggttgaccc gactgctcga
     4261 ggagcccttc tcacggtacg gcgtggacga gctcggcacc gctccgaccg cgaaggtgca
     4321 cgagaccgac gacgagctcg tggtctcgat cgaggtaccc ggattcgacc gcgacgagct
     4381 cgatgtccgt ctgactcccg agggcctcac cgtccgcggt gagcgacggg ccgaggccga
     4441 cgacgaatac atccaccgcc acttcatcct gaccgtgccg ctgcctcccg gcctcgatct
     4501 cgatcggccg gaggcgcgga tcagccgggg cgtcctcacc atacgctttc cgaggacccc
     4561 gacccggccc ggggctcgtc gcatcccggt ccgggcctga gaggacgcat ggccatggcc
     4621 aaggtgatcg gcatcgacct cggcaccacc aactcggtcg tggcggtgat ggaaggtgga
     4681 gagcccgccg tcatctccaa ccaggaagga agtcgactca ccccgtcggt ggtcgccttc
     4741 accaaggacg gcgagatcct cgtcgggcac gtggctcgtc ggcaagccat cacgaacccc
     4801 gagaacaccg tgttctccat caagcggttc atggggcggc gctacgacga ggtgctcgag
     4861 gagatcaaga aggtgcccta caaggtggtc aaggccgcca acggggacgt gcgcgtcgag
     4921 atccgcggca agctctactc gccccccgag atctccgcga tgatcctgcg caagctcaag
     4981 gagtcggccg aggcccacct cggagacaag gtcacccagg cggtgatcac ggtgcccgcc
     5041 tacttcaacg acagccagcg tcaggccacc aaggacgccg gcgccatcgc ggggctcgag
     5101 gtgctccgca tcatcaacga gcccaccgcg gccgccctgg cctacggcct cgacaagaag
     5161 gcggacgagc agatcgcggt ctacgatctg ggtggcggta ccttcgacat ctcgatcctc
     5221 gagatcggac agggcgtctt cgaggtcaag gccaccaacg gcgataccca cctgggcggc
     5281 gacgacttcg accagcgggt catcgactgg atcgcggacg agttcaagaa ggagaaccgc
     5341 atcgacctgc gccaggaccg catggcgctg cagaggctca aggaggcggc cgagaaggcc
     5401 aagatcgagc tgagctcgac gctgcagacc gagatcaacc tgccgttcgt caccgcggac
     5461 gccaccggac ccaagcacct ggtgatgacc ctgacgcggg cccggctcga ggcgctggtg
     5521 gcggacctgg tcgagcgcac catcgagccc tgccgtcgcg ccatgaagga cgccgacgtc
     5581 accgaggcgg acatcgacga ggtgatcctg gtcggcggcc agacccgcat gccgaaggtg
     5641 caggagaccg tcaagcagct cttcggcaag gagccccaca agggcgtgaa ccccgacgag
     5701 gtggtcgcgg tcggcgcggc ggcgcagggc ggcgtgctct ccggcgaggt caaggacgtc
     5761 ctgctcctcg acgtgacgcc gctgtcgctc ggcatcgag
//
LOCUS       DATMCV010000789         4494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_241437, whole genome shotgun sequence.
ACCESSION   DATMCV010000789 DATMCV010000000
VERSION     DATMCV010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241437"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /gene="speB"
                     /locus_tag="VNU03_18085"
     CDS             complement(<1..902)
                     /gene="speB"
                     /locus_tag="VNU03_18085"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HWP76434.1"
                     /translation="MSDWPRPRDGFASPRFAQPATFMRLPHVESPAGLDVALCGIPFD
                     GGCSYRSGARMGPRHVREQSSLIRPWNSALRVHPFERLRVADCGDVDVVPVSIEHTFA
                     AIERTLAPVVEARAVPLCVGGDHTVTLAVLRALARRHGRLGLVHFDAHPDTWDRYFGL
                     PYYHGSTFRRAMEEGLIDGRRSIQVGIRGPLYGPEDFDFHREHGFEVIRIEDVKEGGV
                     APVVERLRRLAGGPVYCSFDVDAADPAYAPGTGTPEAGGLTSYEALSLVRGLRGLELA
                     GGDIVEVSPPYDGPGAITGVLAANL"
     gene            complement(925..2163)
                     /locus_tag="VNU03_18090"
     CDS             complement(925..2163)
                     /locus_tag="VNU03_18090"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWP76435.1"
                     /translation="MNLGGDPMNLGERCRRDFPILSRIVNGHPLVYLDSAASSQKPTA
                     VIEAIQRYYETSHANVHRSIHTLGEEATERYEGARDAVRAFIGARGREEVIFTRGTTE
                     SINLVAQAVGRTLAPGDEIVVSELEHHSNLIPWQMVCRDRGTVLRAVPVLAEGYLDLE
                     AYGRLLSPRTRVVALAHVSNVLGTINPVAQMVEQAHRVGALVLLDGAQAVPHLPVDVT
                     AIGADFYAFSGHKMLGPTGIGVLHGRHEILERLEPAWGGGEMIKEVWIDHAQWNDLPW
                     RFEPGTPPIAGAVGLHAAVEYLGKLGMGQVSAHEQALTAATMEALGRIPGVSLYGPSN
                     PEMKGAVVAFNVDGIHPHDGAALLDQHGIAVRAGHHCAQPLMKCLGIAGTLRASFSVY
                     STPQDVQRLADAVATLRGEL"
     gene            complement(2160..2531)
                     /locus_tag="VNU03_18095"
     CDS             complement(2160..2531)
                     /locus_tag="VNU03_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HWP76436.1"
                     /translation="MALYSNVIRDRFRRPRYRGTLERPDVSGEDVNPLCGDRVRIQAN
                     LADGRLVEGRWTGDCCAISAASADVLLEMATGQPASEVRELTIESILERLDSDIRPSR
                     MKCVALPLTVLQGALTNEVTP"
     gene            complement(2534..3586)
                     /locus_tag="VNU03_18100"
     CDS             complement(2534..3586)
                     /locus_tag="VNU03_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76437.1"
                     /translation="MRAIAIRKEAAPAVAALEGRIACHDVRDEAGRIAISKGQTLDAD
                     AAARLLALPWRDPEVHLLELDPGDVHEEPAGSRLATAACGEQVEVKGYSGGQWTLVGQ
                     RRGLVRVNVDALAAVNALEGMSVFTLYDLQPVEAGEVVARAKITPLAIPERLVKDAES
                     RAWSAGGLVAVKTFRPHVLGALSRETLAPPQRQRFESSLTEKVDWFGSRLLPVRYTPG
                     DAGAVAAEMESLLAAGAQILIAAGAGSLDPLDPVFAGLERLGGRMIRHGAPAHPGSLL
                     WIADLRGHPVLGMPACGMFSQATTFDLVLPRILAGEPGGATELAALGHGGLLSRDSAY
                     RFPPYRKSGARGELSE"
     gene            complement(3597..>4494)
                     /locus_tag="VNU03_18105"
     CDS             complement(3597..>4494)
                     /locus_tag="VNU03_18105"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HWP76438.1"
                     /translation="ADVMDKNAPRLLELNLGDEEAWEIGLTCGGTIEVFVEPVTLGRT
                     DDATLAFYEGARAHAETGGRAAIVTRLDPPHNGGKLLILDRGDRQGSLGDPFLDERFA
                     AEARQALEAGKSRTLFLENVRAFAEVFTPPAILLVVGASHVAMPLTVMAKELGFRTIV
                     IDGRPRFATPERFPHVDALRIGVPSELVQQVPLIPTTALVLVAHDYKYDLPVLRHALS
                     TPVGYIGLLGSTRRGAAILNLLREDGVGEEALARVRVPIGLDLGAQSAPEIALSILAE
                     ALAAQRNATGMPISEKVRRGLK"
BASE COUNT          610 a         1662 c         1621 g          601 t
ORIGIN      
        1 aggttcgcgg cgaggacgcc ggtgatggcg cccggcccgt cgtagggggg cgagacctcg
       61 acgatgtcgc cgccggccag ctcgagcccg cgcaggcctc gcaccaggct cagggcctcg
      121 tacgaggtga ggccgcccgc ctcgggggtg ccggtgccgg gggcgtacgc gggatccgcc
      181 gcgtcgacgt cgaacgagca gtagaccggc ccgcccgcca ggcggcgcag gcgctccacc
      241 accggggcca cgcccccctc cttcacgtcc tcgatgcgga tcacctcgaa gccgtgctcg
      301 cggtggaagt cgaagtcctc cggcccgtag agcgggccgc ggatccccac ctggatcgag
      361 cgccggccgt cgatcagccc ctcctccatc gcgcggcgaa acgtactgcc gtggtagtag
      421 ggcagcccga agtagcggtc ccaggtgtcg gggtgcgcgt cgaagtgcac gagcccgagc
      481 cgcccgtgcc ggcgcgccag cgcgcgcagg accgcgaggg tcacggtgtg gtcgccgccc
      541 acgcagagcg gcaccgcgcg cgcctccacc accggggcca gcgtccgctc gatggccgcg
      601 aaggtgtgct cgatcgagac cggcaccacg tcgacgtcgc cgcagtcggc gacccgcagc
      661 cgttcgaacg gatgcacccg cagcgcggag ttccacggcc ggatcaggga ggactgctcg
      721 cgcacgtgac gcggacccat ccgcgcgccg gaccgatagg agcagccgcc gtcgaacggg
      781 atgccacaga gggccacgtc gagcccggcg ggcgactcca cgtggggcag ccgcatgaac
      841 gtcgccggct gcgcgaagcg gggcgacgcg aagccgtcgc ggggtcgggg ccagtcgctc
      901 acgatggcct ccgcgaggcc gcggctagag ctcgccgcgc agcgtggcca ccgcgtccgc
      961 caggcgctgc acgtcctggg gggtgctgta gaccgagaag ctcgcccgca gcgtgcccgc
     1021 gatgccgagg cacttcatga gcggctgggc gcagtggtgt cccgcgcgca ccgcgatgcc
     1081 gtgctggtcg aggagcgccg cgccgtcgtg cgggtggatg ccgtcgacgt tgaacgccac
     1141 caccgcgccc ttcatctccg ggttcgaggg cccgtagagc gagacgccgg gaatgcggcc
     1201 cagcgcctcc atggtcgccg cggtcagcgc ctgctcgtgg gcgctcacct gccccatgcc
     1261 gagcttgccc aggtactcga ccgcggcgtg gaggcccacc gcgcccgcga tcggcggcgt
     1321 gcccggctcg aagcgccacg gcaggtcgtt ccactgggcg tggtcgatcc agacctcctt
     1381 gatcatctcg cctccgcccc aggcgggctc gaggcgctcg aggatttcgt ggcggccatg
     1441 cagcactccg atgccggtcg ggccgagcat cttgtggccg gagaacgcat agaagtccgc
     1501 gccgatcgcg gtcacgtcga ccggcaggtg cggcaccgcc tgcgcgccgt cgagcagcac
     1561 cagcgcgccg acccggtgcg cctgctcgac catctgcgcc accgggttga tggtgccgag
     1621 cacgttggag acgtgggcca gggccaccac ccgggtgcgc ggcgagagca gccgtccgta
     1681 ggcctcgagg tcgagatagc cctcggccag gaccgggacc gcccgcagca ccgtgccgcg
     1741 gtcgcggcat accatttgcc acgggatcag gttcgagtgg tgctcgagct cgctcaccac
     1801 gatctcgtcg cccggggcca gggtgcggcc caccgcctgg gccacgaggt tgatcgactc
     1861 ggtggtcccg cgcgtgaaga tgacctcctc gcggccgcgc gccccgatga aagcgcgcac
     1921 cgcgtcgcgt gcgccctcgt accgctcggt ggcttcctcg ccgagggtgt ggatcgagcg
     1981 gtgcacgttg gcgtggctgg tctcgtagta gcgctggatc gcctcgatca ccgcggtcgg
     2041 cttctggctc gacgcggccg agtccaggta gacgagcggg tgtccgttga cgatccgcga
     2101 gaggatgggg aagtcgcgcc ggcagcgctc gcccaggttc atggggtcac ctcccaggtt
     2161 catggggtca cctcgttggt gagcgcgccc tgcagcaccg tgagcggcag ggccacgcac
     2221 ttcatccggg acgggcggat atcggagtcg agccgctcga ggatgctctc gatcgtcaac
     2281 tcgcggacct ccgacgcggg ctggcccgtc gccatctcca gcagcacgtc cgcggacgcg
     2341 gccgagatgg cgcagcaatc gccggtccac cgcccctcga cgagccgccc gtcggcgagg
     2401 ttcgcctgga tgcgcacgcg gtctccgcag agcgggttca cgtcctctcc cgagacgtcc
     2461 ggccgctcca gggtgccacg gtaccgcggc cggcggaacc ggtcgcggat gacgttgctg
     2521 tagagcgcca tcgtcactcc gacagctccc cgcgcgcgcc gctcttgcgg tagggcggga
     2581 agcggtaggc gctgtcgcgc gacaggaggc ccccgtggcc gagcgcggcc agctcggtcg
     2641 cgccgcccgg ctcgccggcc aggatgcggg gcaggaccag atcgaaggtg gtggcctgcg
     2701 agaacatccc gcacgcgggc atgccgagca ccggatgacc gcgcaggtcc gcgatccaga
     2761 gcaggctgcc cggatgggcg ggcgccccgt ggcggatcat ccgcccgccg agccgctcga
     2821 gaccggcgaa gaccggatcg agcggatcga gtgatccggc gccggccgcg atcaggatct
     2881 gagcccccgc ggcgagcagg ctctccatct cggccgcgac cgcccccgcg tcgcccgggg
     2941 tgtagcgcac cggcaggagg cgcgagccga accagtccac cttctcggtc agcgaggact
     3001 cgaaccgctg gcgctggggc ggggccaggg tctcccggct cagcgcaccc agcacgtgcg
     3061 ggcggaacgt cttcaccgcg accaggccgc ccgcgctcca cgcccgcgac tcggcgtcct
     3121 tcacgagccg ctccgggatc gcgagcgggg tgatcttggc tcgcgcgacg acctcgcccg
     3181 cctccaccgg ctggagatcg tagagggtga acaccgacat gccctcgagc gcgttcaccg
     3241 ccgcgagcgc gtccacgttg acccgcacga ggccccggcg ctggcccacg agcgtccact
     3301 gcccgcccga gtagcccttc acctcgacct gctcgccgca cgccgccgtg gccagccgcg
     3361 acccggccgg ctcctcgtgc acgtcgcccg ggtccagctc gagcaggtgc acctccgggt
     3421 cccgccacgg gagcgcgagc agccgggccg ccgcgtccgc gtcgagggtc tgtcccttgg
     3481 agatcgcgat ccgtccggcc tcgtcccgca cgtcgtggca ggcgatgcgc ccttcgagcg
     3541 cggcgacggc gggcgcggcc tccttccgga tcgcgatggc tctcatggcg cgcgccttac
     3601 ttgaggcctc ggcggacctt ctcggagatc ggcatgccgg tggcgttgcg ctgggccgcc
     3661 agggcctcgg ccaggatgct gagcgcgatc tcgggcgcgg actgggcgcc caggtcgagc
     3721 ccgatcggca cccgcacccg cgcgagcgcc tcctcgccca ccccgtcttc ccgcagcaga
     3781 ttcaggatgg cggctccgcg ccgcgtgctg ccgaggagcc cgatgtagcc gaccggcgtg
     3841 ctcagcgcgt ggcgcagcac cggcaggtcg tacttgtagt cgtgcgccac cagtaccagc
     3901 gcggtggtcg ggatcagcgg cacctgctgc accagctcgg agggcacccc gatccgcagg
     3961 gcgtccacgt gcgggaagcg ctccggggtc gcgaagcggg gccgcccgtc gatcacgatg
     4021 gtccggaagc cgagctcctt ggccatcacg gtcagcggca tcgccacgtg gctcgcgccc
     4081 accaccagga gaatggcggg cggggtgaag acctccgcga aggcccgcac gttctcgaga
     4141 aagagggtgc gcgacttccc cgcctccagc gcctgccggg cctccgccgc gaagcgctcg
     4201 tcgaggaatg gatcgcccag gctgccctgg cggtcgccgc gatcgaggat caggagcttg
     4261 cctccgttgt ggggtggatc gagccgcgtg acgatcgcgg cgcggccccc ggtctcggcg
     4321 tgagcccgcg caccctcgta gaaggcgagc gtcgcgtcgt cggtccggcc gagcgtcacc
     4381 ggctccacga agacctcgat ggtcccgccg caggtgagcc cgatctccca cgcctcctcg
     4441 tcgcccaggt tcagctcgag cagccgcggg gcgttcttgt ccatcacgtc ggcg
//
LOCUS       DATMCV010000790         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_309543, whole genome shotgun sequence.
ACCESSION   DATMCV010000790 DATMCV010000000
VERSION     DATMCV010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309543"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VNU03_18110"
     CDS             <1..547
                     /locus_tag="VNU03_18110"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76439.1"
                     /translation="ARLADGTDQGLAAFRDTVARDPRFSINVLREPEYYAKQAGVLSS
                     LINVLGYTVASFMAVGATFGALNCMYSAIASRQVEIATLRAIGFGGTPVVVSVVIEAL
                     LLALLGGAVGGALAYVYCDGASLSTLNFSTFSQVGFDFRVTPGLLLRGLVWALVIGAA
                     GGLPPAVRSARIPVTEALRTL"
     gene            639..2339
                     /locus_tag="VNU03_18115"
     CDS             639..2339
                     /locus_tag="VNU03_18115"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76440.1"
                     /translation="MHDLVIDNARLIDGLGTPAADGSLAVKDGRIAAISADRRGADLG
                     AAGARVDAQGLVLAPGIVDLHTHYDAQLTWDPYATPSTALGVTTVVIGNCGFTIAPCH
                     PAHRDLIVRNLTHVEGMSLEAMRAGINWDFESYPEYLASLERRGLVPNVASFVGHSSV
                     RTYVLGEDASKRKATDAEIAEMRRIVVEALRTGAIGFATSTLEQHNGENGIPMPSRLA
                     DEREMTALTGALGEVGRGIFMLTKGMTSTVPWLESIAAANGRPVMIAAMFVDPGDPTR
                     VFRELSEIEAARSRGRELWAQVGCFPLGMEFTLRHPYPLEAFLAWRPAIVAHDEARYR
                     AVLADPSFRDGLKQEIGQPGVPNRFSDKNWDHLTIAEVKRPEHRSLEGKTIGTLARER
                     GQHPWDTFLDFGLDGELDAMFDCRLFNIDENEVRRLLRHPNAAVALSDAGAHLSFLCD
                     AGFGLHLFGHWARERGDLTLEQAVRTVTSAVADAYRIKDRGRLTPGAWADLMLFDPAT
                     VGRGGKRRVHDLPTGASRLDTPAVGLHGVWVNGVRTVDERGVVKECGRPGRLLREFAP
                     "
     gene            2538..>2782
                     /locus_tag="VNU03_18120"
     CDS             2538..>2782
                     /locus_tag="VNU03_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76441.1"
                     /translation="MVGSTMGMSLALVAPNAWAQTPPPSAEQKEGKDDVALPGVDVRS
                     QRNQFKIEEPSLYKLPDPIKDTPQSITVIPEQVLDERA"
BASE COUNT          396 a         1031 c          948 g          407 t
ORIGIN      
        1 cgcgcgcctc gccgacggca ccgaccaggg cctggctgcg ttccgggata ccgtcgcccg
       61 cgacccgcgc ttctcgatca acgtgctccg cgagccggag tactacgcca agcaggcggg
      121 cgtgctgtcc agcctcatca acgtgctcgg ctacacggtg gcctcgttca tggcggtggg
      181 cgcgacgttc ggggcgctca actgcatgta ctccgcgatc gcgagccgcc aggtcgagat
      241 cgccaccctg cgcgccatcg gcttcggcgg cacgccggtg gtggtctcgg tggtgatcga
      301 ggcgctcctg ctcgcgctgc tgggcggcgc ggtcggcggc gcgctcgcct acgtctactg
      361 cgacggggcg tcgctctcca cgctcaactt cagcaccttc tcccaggtgg ggttcgactt
      421 ccgggtcacg ccggggctgc tcctccgcgg cctcgtctgg gcgctcgtga tcggtgccgc
      481 cggcggcctg ccgcccgcgg tgcgctcggc ccgcatcccg gtcaccgagg ccctgcgcac
      541 actctagccg cgccgccggc ccgctgcggt tgactcgccg tcgcggccgg gctagcctcg
      601 accggtcagg cagcccacat ccccaggagg ctccaccctt gcacgacctc gtgatcgaca
      661 acgcccgcct catcgatggt ctcggcacgc ccgcggccga cggcagtctc gcggtgaagg
      721 acgggcgcat cgccgcgatc agtgccgacc gtcgcggtgc ggacctgggg gccgcgggcg
      781 cgcgcgtcga cgcgcagggc ctcgtgctcg cgcccgggat cgtggacctg cacacccact
      841 acgacgctca gctcacctgg gacccctacg cgacgccgtc gaccgcgctc ggcgtcacca
      901 cggtggtgat cggcaactgc ggcttcacga tcgcgccgtg ccatccggcg caccgcgacc
      961 tgatcgtgcg caacctgacc cacgtggagg gcatgtcgct cgaggcaatg cgggccggca
     1021 tcaactggga cttcgagtcg tatccggagt acctggcctc cctggagcgg cgcggtctgg
     1081 tgcccaacgt ggcctccttc gtgggccact cctcggtgcg cacctacgtg ctcggcgagg
     1141 acgccagcaa gcggaaggcg accgacgcgg agatcgcgga gatgcgccgc atcgtggtgg
     1201 aggccctgcg gacgggcgcc atcggcttcg ccacctccac cctcgagcag cacaacggcg
     1261 agaacggcat ccccatgccg tcgcgcctgg ccgacgagcg cgagatgacc gcgctgaccg
     1321 gcgcgctcgg cgaggtcggc cgcggcatct tcatgctgac caagggcatg acctccaccg
     1381 tgccgtggct cgagtccatc gcggcggcca acggccgccc ggtgatgatc gcggccatgt
     1441 tcgtggatcc gggcgacccg acccgcgtgt tccgcgagct ctccgagatc gaggccgccc
     1501 gcagccgggg gcgcgagctg tgggcgcagg tggggtgctt cccgctcggc atggagttca
     1561 cgctgcgcca cccctatccg ctcgaggcct tcctcgcctg gcggcccgcg atcgtggcgc
     1621 acgacgaggc acgctaccgg gcggtgctcg ccgatccgtc gttccgcgac gggctcaagc
     1681 aggagatcgg ccagcccgga gtgcccaacc gcttcagcga caagaactgg gatcacctca
     1741 ccatcgcgga ggtgaaacga ccggagcacc ggagcctcga gggcaagacc atcgggaccc
     1801 tggcccgcga gcgcggccag catccgtggg acaccttcct cgacttcggg ctcgacgggg
     1861 agctggacgc gatgttcgac tgccgtctct tcaacatcga cgagaacgag gtgcgccggc
     1921 tcctgcggca tcccaacgcc gcggtggccc tctccgacgc gggggcgcat ctctccttcc
     1981 tctgcgacgc gggcttcggc ctccacctct tcggccactg ggcgcgcgag cgcggcgacc
     2041 tcaccctcga gcaggcggtg cggacggtga ccagcgcggt ggccgacgcc taccggatca
     2101 aggaccgggg ccggctcacc cccggagcct gggccgacct catgctcttc gaccccgcga
     2161 cggtcgggcg cgggggcaag cggcgcgtcc acgacctgcc gaccggggcg agccgcctcg
     2221 atacgccggc ggtgggcctc cacggggtgt gggtcaatgg ggtgcgcacg gtggacgagc
     2281 gcggggtcgt caaggagtgc ggccgtcccg gccgcctgct ccgcgagttc gcgccctaga
     2341 attagactct agaattaggg ttgacaaccc tcgctagtta gggttaccta acgacgccct
     2401 gctagccagc tccggttcct cgacctacgc gagccagccc ggccatctca tccttgggag
     2461 gcgtggcaat gggtcgcaat cgatcgttcc ggaggcatat cgcgcgggct agacgtcgcg
     2521 ggcacgcgct ccaccgcatg gtcgggtcca ccatggggat gagcctcgcg ctcgtcgccc
     2581 cgaacgcgtg ggcccagacg ccgccaccgt ccgccgagca gaaggaaggc aaggacgacg
     2641 tcgccctgcc cggcgtcgac gtccgcagcc agcgcaacca gttcaagatc gaggagccga
     2701 gcctctacaa gctccccgac ccgatcaagg acaccccgca gtccatcacc gtcatcccgg
     2761 agcaggtgct cgacgagcgc gc
//
LOCUS       DATMCV010000791         3034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_508994, whole genome shotgun sequence.
ACCESSION   DATMCV010000791 DATMCV010000000
VERSION     DATMCV010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508994"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1023)
                     /gene="dapA"
                     /locus_tag="VNU03_18125"
     CDS             complement(142..1023)
                     /gene="dapA"
                     /locus_tag="VNU03_18125"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017352151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWP76442.1"
                     /translation="MRFSGVWLPAITPFIRGEIDYPAYERLIDHYVKLGVTGIIPLGT
                     TGESPTIDDEEADRVVERTVATVAGRVPIVVGVGGNDTRKAVKAVKRLQRHPVQGILS
                     VCPYYNRPSQDGMHEHFTRIAEATDRPILIYNIPYRTGVNLTNETLLRLAEIPNIAGV
                     KDSSGSIAQSLDLLRQRPDGFAVLTGEDAYFYTMLAHGGDGGILAASHVETALFLTVY
                     ERMAANDHQGARKAWSRLEALVPLLFREPNPMPIKHCLWRQGLIRSPECRLPLTRVSS
                     ALVGKLDRLVAEIGDSA"
     gene            1105..2109
                     /locus_tag="VNU03_18130"
     CDS             1105..2109
                     /locus_tag="VNU03_18130"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP76443.1"
                     /translation="MRVDQLTEHLPAEEIERPKAWTRDTISADRWLISLPEACIAELD
                     QVVMSLRATPRPVESLAPAAFSLRACSDLMSDVRAKLETDTGLAVVDRFPVERYETEE
                     NRAIGWLLAAMLGQVVAQKANGARLYDVKDTGQALGYGVRRSVTNLGQPFHTDGGWLW
                     KAPNFVGLFCLESALEGGLSRFTSLVTAHNELRRTRPDLLARLYTPFAWDRQGEHAPD
                     DVRASHHPVFEYERGVLAARYYEDYIVNGQQLAATALDDEGREALDAMRAIVDDPASW
                     VEFRIEKGQLQYLNNRQFAHSRTAFKDAGTAQQGGGRHMLRLWNRDEGSWHLEGRPAA
                     "
     gene            2117..2848
                     /locus_tag="VNU03_18135"
     CDS             2117..2848
                     /locus_tag="VNU03_18135"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWP76444.1"
                     /translation="MPPLTLETAAYSALILLLAYWVRGMAGFGSGLIAVPLLTLLWPV
                     TVVVPLVVALDYLGSASQGVRNVEQVAWREQLWLIPFMVVGVLAGLWVLAAMSTAILA
                     RILGGFVIVYAIYQMLPLPELRGSRAAASYCGLLGGLVGTLFGTGGPFYVIYLNLRGL
                     ERLAFRATFAMNFLIDGGVRLAAFAAAGLFGRQTLGYLLAALPIAGAGLYVGGRIQTG
                     LSQRTFQIVIRVLLLTSGLALLLKR"
     gene            complement(2838..>3034)
                     /locus_tag="VNU03_18140"
     CDS             complement(2838..>3034)
                     /locus_tag="VNU03_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76445.1"
                     /translation="RGALLGLIALTNQGGFVIGSAVGAAAIGSGGYRGMALAALAQGL
                     IAAVLALPLLRRPRRARVSA"
BASE COUNT          411 a         1076 c         1049 g          498 t
ORIGIN      
        1 cgcggcggcg ctgccgcaat ttatccaccg cgcgccgctg cgcatccgtt gccattcagg
       61 cctcccacga tccgtggata tatatccagg gatcgattac gcttcaagcc gtatcctccc
      121 tcgaggtctt gtttgccgcg gtcacgcgct gtctccgatt tctgcgacca gccgatcgag
      181 cttgccgacg agcgctgacg acactcgcgt cagcggcaag cggcattccg gcgagcggat
      241 gaggccctgg cgccacaggc agtgcttgat cggcatcggg ttcggctccc tgaagagcag
      301 gggcaccagc gcctcgagcc gggaccaggc cttgcgcgcg ccctgatggt cgttggcggc
      361 catgcgctcg tacacggtga ggaacagcgc ggtctcgacg tgcgacgcgg cgaggatgcc
      421 gccgtcgccg ccgtgggcga gcatggtgta gaagtaggcg tcctcgccgg tgagcaccgc
      481 gaagccatcg ggccgctgac gcagcagatc gagcgattgc gcgatgctgc ccgacgagtc
      541 cttcaccccg gcgatgttcg ggatctcggc caggcggagc agggtctcgt tggtcaggtt
      601 gacgccggtc cggtagggga tgttgtagat caggatcggc cggtcggtgg cctccgcgat
      661 gcgcgtgaag tgctcgtgca tgccatcctg gctcggccgg ttgtagtacg ggcagaccga
      721 gagaatgccc tggaccgggt ggcgctgcag ccgcttcacc gccttgaccg ccttgcgcgt
      781 gtcgttgccg ccgaccccga ccacgatcgg cacccggccc gccaccgtcg cgacggtgcg
      841 ctcgaccacg cggtcggcct cctcgtcgtc gatggtcggg ctctcgccgg tggtgccgag
      901 cgggatgatg ccggtgaccc cgagcttcac gtagtggtcg atgaggcgct cgtacgcggg
      961 gtagtcgatc tccccccgga tgaacggggt gatcgcgggc agccagacgc cggagaagcg
     1021 catgggtggt ggtacctcct ggattttcga gtctcgcacg cgtgctatac tcccgggcac
     1081 ttccgacgta gaaggaggct cgcgatgcgt gtcgaccagc tgacggagca cctcccggcc
     1141 gaagagatcg agcgtccgaa ggcgtggacg cgcgacacca tctccgccga ccggtggctg
     1201 atctcgctcc ccgaggcgtg catcgcggag ctggaccagg tggtgatgtc gctgcgggcg
     1261 accccgcgcc cggtcgagag cctggccccc gcggccttct cgctgcgagc gtgctccgat
     1321 ctcatgtcgg acgtgcgggc gaagctcgag accgacaccg gcctcgcggt ggtggaccgc
     1381 ttcccggtcg agcgctacga gacggaggag aaccgggcga tcggctggct gctcgccgcc
     1441 atgctcgggc aggtcgtcgc gcagaaggcc aacggggccc ggctctacga cgtgaaggac
     1501 accggccagg cgctcggcta cggggtgcgg cgatcggtca ccaacctggg ccagccgttc
     1561 cacaccgacg gcggctggct ctggaaggcg cccaacttcg tgggtctctt ctgtctcgag
     1621 tccgcgctcg agggcggcct cagccgcttc acgagcctgg tgaccgccca caacgagctc
     1681 cgacgcaccc ggcccgacct cctcgcccgc ctctatacgc cgttcgcgtg ggatcgccag
     1741 ggcgagcacg cccccgacga cgtccgcgcc agccatcacc cggtgttcga gtacgagcgc
     1801 ggggtcctgg ccgcgcgcta ctacgaggac tacatcgtca acggccagca gctcgccgcc
     1861 accgcgctcg acgacgaggg gcgcgaggcg ctcgacgcga tgcgcgcgat cgtggacgat
     1921 cccgccagct gggtcgagtt ccgcatcgag aagggtcagc tccagtacct caacaaccgc
     1981 cagttcgcgc acagccggac cgccttcaag gacgcgggca ccgcgcagca gggtgggggc
     2041 cgtcacatgc tccggctctg gaaccgcgac gagggctcct ggcatctcga gggtcgcccc
     2101 gccgcctgat cgcttcgtgc ccccgctgac cctcgagacc gcggcctatt ccgcgctgat
     2161 cctcctgctc gcctactggg tgcggggcat ggccggcttc ggctccggcc tgatcgcggt
     2221 gccgctgctc accctgctgt ggccggtcac cgtcgtcgtg cccctcgtgg tcgcgctcga
     2281 ctaccttggc tcggccagcc agggcgtgag gaacgtggag caggtggcct ggcgcgagca
     2341 gctctggctc atcccgttca tggtggtggg cgtgctcgcc ggcctctggg tgctggccgc
     2401 gatgagcacc gcgatcctgg cccgcatcct gggcggcttc gtcatcgtgt acgcgatcta
     2461 ccagatgctg ccgctgcccg aattgcgcgg ctcgcgcgcg gccgcgagct actgcgggct
     2521 gctgggcggg ctggtcggca cgctctttgg caccggcggg cccttctacg tgatctacct
     2581 gaacctgcgt gggctcgagc gcctcgcctt ccgcgccacc ttcgcgatga acttcctgat
     2641 cgatggggga gtccggctcg ccgcgttcgc ggccgcgggg ctgttcggcc gccagacgct
     2701 cggctatctg ctggccgcgc tccctatcgc gggggccggc ctctacgtgg gcggccgcat
     2761 ccagaccggg ctctcgcagc gcacgttcca gatcgtgatc cgcgtgctcc tcctcacctc
     2821 cggcctggcc ctcctgctca agcgctgact cgcgcgcgcc gaggccgcct gaggagcgga
     2881 agggccagca cggccgcgat caggccctgg gccagcgcag cgagcgccat gccccggtag
     2941 ccgccgctcc cgatcgcggc cgcgcccacc gcggagccga tcacgaagcc gccctggttc
     3001 gtgagcgcga tcagcccgag gagtgcgccg cgct
//
LOCUS       DATMCV010000792         5720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_978362, whole genome shotgun sequence.
ACCESSION   DATMCV010000792 DATMCV010000000
VERSION     DATMCV010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978362"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1229)
                     /locus_tag="VNU03_18145"
     CDS             complement(60..1229)
                     /locus_tag="VNU03_18145"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76446.1"
                     /translation="MLTRRQFTTTLGGAMAAGLLAPRAGRAQSAPIKLGSIFSYTGPA
                     AFLGDRMKRSVELFVEEANKKGGLGGRRIELTLYDAGSESTKAVLAAKRLIEQDGVEA
                     IVGDGNRSDIGLALVPSVQRAEVPLMSVSGATALVEPVKERGWVFKSTVNDVEVVARL
                     LDFFKKRGIKDVAMLNDTGAFGVSARDQLRAQAPAAGLNVVYEEFGPGDNDLTTQLTR
                     IRGSNAKAIVCWTVTPAGVVFLKNAQQLGLKQMLVHGFGFVADRYMQLAGDAAEGLML
                     TSLKFPVAEQLPDADPIKATILDYKARYRARYNEETDVYGGQAWDGIGMVAQAITRAG
                     SSERGKIRAGLEEHVREYKGVGGVFTFSPQKHWGLAKSDVVMIEWRQGKFRLVDL"
     gene            complement(1280..2161)
                     /locus_tag="VNU03_18150"
     CDS             complement(1280..2161)
                     /locus_tag="VNU03_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76447.1"
                     /translation="MSSANLLQFLFSGLMVGAIYALVALGLNVVFNATGAVNFAQGEF
                     SMLGGMLGAAMLSATGLPLALVFASTVVVVTAIGVGMERLIVRPVRHADVLNLIIVTI
                     GASILIKGGVMVTLGKNAAGLPAFTGERPFQILGATFVPQALWILGVAAAIVVALHFF
                     FDRTLLGRAMRAVASDREAARLMGIDVGRIVMLSFALAAAVGAIGGLIVTPVTLTIYD
                     AGTMLGLKGFAAAVTGGLGNTFGGIAGGLVLGLLEAFGGGLIGSEFKDVAAFVLLLLL
                     LFLRPRGLFARGESERV"
     gene            complement(2215..4194)
                     /locus_tag="VNU03_18155"
     CDS             complement(2215..4194)
                     /locus_tag="VNU03_18155"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWP76448.1"
                     /translation="MTMRRVILFIALFLVVLGLPGGAAAPPPGGSAARRVIIFVWDGL
                     RADDVTQENMPNYFALARSGVVFADHHAVYPTFTMMNSASIATGTYPGVHGFYGNVVY
                     APNAKGKNAKGVDIDFSAPAFIEDFGVVEAVRDSYQGALTRVSTMLQAAQAKGLTTAA
                     VGKFGAAFIQDYHRGGIILDEDAAIPLSFAKELQNAGYALPKNTVNAYRAGTLVLAGS
                     NGDPTAPIPIQRLNDRETANPLDRTGALSRRGFTYLTEVFVNYILPTKQPDLTIFWSK
                     EPDATSHAYGPGTYNSIDATKMNDEILGRVVDKIIQLGWEQTTDIIITQDHNHSTVSG
                     DIAHYPLRGIADGRVGSHDPYGYSVSGFVRTAALLALDGLKAYDGAGCRNIPTLSGIL
                     FDGTPLHPTKDDADGTVCGKAQTYTSPRYAAPKPLPPGGIVVAANAGSDYLFVPDGDV
                     NTVKAAVVSLQSRPQFGAVFVSDKYGDIAGTLRMGLINVESPASGRGPDIIVSFSFDE
                     HVAVAGRPGISYASSVNRRGDHGSFSRTDTHISMMAHGPDFKSGMYDPLPTANVDIAP
                     TVARILALNMPGTQGRALEEALKDGPRVTEYAVLGKIYPSSKKGGLKMKLSTDLDGRS
                     IDPNLTTFSVELTTKTLTRGSSSYMYFDQARAIRE"
     gene            complement(4256..5416)
                     /locus_tag="VNU03_18160"
     CDS             complement(4256..5416)
                     /locus_tag="VNU03_18160"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76449.1"
                     /translation="MKNGFKVMDSDLHTMEPDGLWERYLAEPFKKFAPRFLRSSERSP
                     NQPVIQIGDLELGEMSKRARTAVVGRDLQQRAFARSPHYEIAHARGYDAETHIQAMNI
                     EGIDGAVLYGTRGRQVLMHDDLDPAVASALARAHNDWTHDYCQHAPSRLKFAAQVAFH
                     DVPGAIAEARRAVRQLGAVAVVGNPNPINGRHVHDPEMEPLWAAIEELGVPVGFHPTG
                     HASLRDDIARRYLDTPNGRVIGVAGRNPVELMMAFGSMAAGGVLERHPGLRCAFLEGT
                     CGWLPWYLWRLDEAWEKFGPGSEIQIAHTPSQYFFRQCVVATDADEKPLRQVIEAIGD
                     DNIVVSTDYPHSDGLFPKAIEEFVHLEGVTDKSKSKILWDNCARLYNLAGSR"
     gene            5394..>5720
                     /locus_tag="VNU03_18165"
     CDS             5394..>5720
                     /locus_tag="VNU03_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76450.1"
                     /translation="MTLKPFFIAGLLVAGGSLGAPEDLPGEAAASRGMLPCGPMKLGT
                     SVRFLFPTSPATHERFRSLVASMPKGAFIERPLGAYAVDEQARNLMEIAAAARAAGLD
                     GLLTGDS"
BASE COUNT          903 a         1890 c         1974 g          953 t
ORIGIN      
        1 gccaggagcg cccggccgga gacccggccg ggcagccgcg ccgcctcgcc cacgcggggc
       61 tagagatcca ccaggcggaa cttcccctgg cgccactcga tcatgaccac gtcgctcttg
      121 gcgaggcccc agtgcttctg cggcgagaag gtgaagacgc cgcccacgcc cttgtactcg
      181 cggacgtgct cctcgagccc ggcgcggatc ttcccgcgct cgctgctgcc cgcgcgggtg
      241 atggcctgcg ccaccatgcc gatgccgtcc cacgcctggc cgccgtagac gtcggtctcc
      301 tcgttgtagc gggcgcggta gcgcgccttg tagtcgagga tggtggcctt gatgggatcg
      361 gcgtcgggga gctgctcggc caccgggaac ttgagcgagg tgagcatcag gccctcggcc
      421 gcgtcgcccg cgagctgcat gtagcggtcg gccacgaagc cgaagccgtg caccagcatc
      481 tgcttgagtc cgagctgctg cgcgttcttc aggaagacca cgcccgccgg cgtcaccgtc
      541 cagcacacga tggccttggc gttgctgccg cggatgcggg tgagctgggt ggtgagatcg
      601 ttgtcgccgg ggccgaactc ctcgtagacc acgttgagcc cggcggccgg ggcctgggcc
      661 cgcagctgat cccgggcgct gaccccgaag gccccggtgt cgttcagcat cgccacgtcc
      721 ttgatgccgc gcttcttgaa gaagtcgagc aggcgggcca ccacctccac gtcgttcacg
      781 gtggacttga agacccagcc gcgctccttc accggctcga cgagcgcggt ggcgccggag
      841 acgctcatca gcggcacctc ggcgcgctgc acgctcggca ccagggccag gccgatgtcg
      901 ctgcggttgc cgtcgccgac gatcgcctcg actccatcct gctcgatcag gcgcttggcc
      961 gcgagcaccg ccttggtgga ctcgctgccc gcgtcgtaga gggtcagctc gatgcgccgg
     1021 ccgccgagcc cgcccttctt gttggcctcc tcgacgaaga gctcgaccga gcgcttcatg
     1081 cggtcgccca ggaaggcggc ggggccggtg taggagaaga tggagccgag cttgatgggg
     1141 gcggactggg cccggcccgc gcggggggcg aggaggccgg cggccatcgc gccgccgagg
     1201 gtcgtggtga actgccgtcg cgtgagcatg ggatctccat accgcgcccg gatgccggcg
     1261 cgccgatcga ggtgagcgct catacccgct cgctctcgcc gcgcgcgaac aggccccgcg
     1321 ggcggaggaa cagcaggagc agaaggagga cgaaggcggc cacgtccttg aattccgagc
     1381 cgatgagtcc gccgccgaag gcctcgagga ggcccaggac gagcccaccc gcgatgccgc
     1441 cgaaggtgtt gccgagcccg ccggtgaccg cggccgcgaa gcccttgagg cccagcatgg
     1501 tgcccgcgtc gtagatggtg agcgtgaccg gggtcacgat gagcccgccg atcgcgccca
     1561 ccgcggccgc gagcgcgaac gacagcatga cgatgcggcc gacgtcgatg cccatgagcc
     1621 gggccgcctc ccggtcgctc gccaccgcgc gcatggcgcg gccgagcagg gtgcggtcga
     1681 agaagaagtg gagcgcgacc acgatggccg ccgcgacccc gaggatccag agggcctggg
     1741 gcacaaaggt cgcgccgagg atctggaagg gccgctcccc ggtgaaggcg ggcaggccgg
     1801 ccgcgttctt gccgagggtg accatcaccc cgcccttgat caggatcgac gccccgatcg
     1861 tcacgatgat caggttcagc acgtcggcgt gccgcaccgg ccgcacgatg agccgctcca
     1921 tgccgacgcc gatcgcggtc accacgacga cggtggaggc gaacacgagg gcgagcggca
     1981 ggccggtcgc ggagagcatc gcggccccca gcatcccgcc cagcatggag aactcgccct
     2041 gggcgaagtt caccgcgccg gtcgcgttga agacgacgtt gaggccgagc gcgacgaggg
     2101 cgtagatcgc gccgaccatg agcccggaga acaggaactg gagcaggttc gcggagctca
     2161 cggatgcccg agcgtaagct cagcccggcg ccttgtcaac gctcgggtcg tacatcattc
     2221 acggatcgct ctggcctggt cgaagtacat gtaactcgag ctccctcggg tgagggtctt
     2281 cgtcgtcagc tcgacggaga aggtcgtcag gttaggatca atgctgcgac cgtcgaggtc
     2341 ggtggagagc ttcatcttca agccgccctt ttttgaggac gggtagatct ttcccaagac
     2401 cgcgtattcc gtgacccgtg gcccgtcctt caaagcctcc tcgagcgctc gtccctgagt
     2461 gcccggcata ttgagcgcga gaatgcgcgc gaccgtaggc gcgatatcga cgttcgcggt
     2521 gggaagcggg tcatacatac ccgacttgaa gtcgggccca tgggccatca tcgaaatatg
     2581 cgtgtcggtg cgtgagaagc tgccgtggtc gcccctgcgg ttgaccgagc tcgcgtagct
     2641 gattccaggc cggccggcca ccgcgacgtg ttcgtcgaag ctgaagctga ctatgatatc
     2701 cgggccgcga ccgctcgccg ggctttccac gttgatgagc cccatgcgca gggttcccgc
     2761 gatgtccccg tacttgtcac tcacgaagac ggcgccgaat tgcgggcgac tctggagact
     2821 caccaccgcg gccttgaccg tgttcacgtc accatcgggg acgaacaagt agtcgctccc
     2881 cgcgttcgcc gccaccacga tgcctccggg gggaaggggc ttcggcgccg cataccgtgg
     2941 cgaggtgtac gtttgcgctt tgccacacac ggttccatcc gcgtcgtcct tggtgggatg
     3001 aaggggcgtg ccgtcgaaca ggatcccgga caaggtgggg atgtttcgac agcccgcgcc
     3061 atcgtaggcc ttgaggccat cgagggcgag caacgcggcc gtccggacga agcccgagac
     3121 ggagtacccg tacggatcat gcgagccgac gcggccatcg gcaattcctc gcaacgggta
     3181 gtgagcaata tcaccggaga cggtgctgtg attatgatcc tgcgtgatga tgatgtcggt
     3241 ggtctgctcc cagccgagct gaataatctt gtccacgacg cgacccagga tctcgtcatt
     3301 catcttcgtg gcgtcgatcg agttgtatgt ccccgggccg tacgcgtggc tcgttgcgtc
     3361 tggctccttc gaccagaaga tcgtgaggtc cggctgtttc gtcgggagga tgtaattcac
     3421 gaagacttcg gtcagatacg taaacccacg ccgggagaga gccccggtgc ggtcgagcgg
     3481 attcgcggtc tctcgatcgt tgagcctctg aatcggaatc ggagcggtgg ggtcgccatt
     3541 gctaccggcg agtactagag ttcccgcgcg gtaggcgttg acggtgttct tcggaagggc
     3601 gtacccggcg ttctggagct ccttcgcgaa actcagcggg atcgccgcgt cctcgtcgag
     3661 gatgatgccc cctcggtggt aatcctgaat gaacgcggcg ccgaatttcc cgaccgctgc
     3721 ggtcgtcagt cctttggcct gcgccgcctg gagcattgtc gacacacgcg tcagtgcgcc
     3781 ctgatacgag tcccggaccg cttccaccac cccgaagtcc tcgatgaacg ccggggcaga
     3841 aaaatcgata tcgactccct tcgcgttctt ccccttggca ttcggtgcgt acactacgtt
     3901 gccatagaac ccgtgcaccc ccggatacgt cccagtggcg atggacgcgg agttcatcat
     3961 ggtgaaggtc gggtagaccg cgtggtgatc cgcgaagacg acccctgatc gcgcgagggc
     4021 aaagtagttc ggcatgtttt cctgggtgac gtcgtccgct cgcagcccgt cccaaacgaa
     4081 gatgatgacc cttcgcgcag ctgaaccgcc cggtggtggt gctgcggcac cacctgggag
     4141 tccgaggaca actaagaaca aggcgatgaa gagaatgact ctccgcattg tcatggctgt
     4201 cctcgcatgt tgaatgcgaa cgttgtcggc ggaagaatgt gctacttctc tcgggctacc
     4261 ggctcccggc caggttgtag agccgcgcgc agttgtccca gaggatcttc gacttcgact
     4321 tatcggtgac gccctccagg tgtacgaatt cctcgatcgc cttcgggaag agaccgtccg
     4381 agtgcgggta gtcggtcgag accacgatgt tgtcgtcgcc gatcgcctcg atgacctggc
     4441 ggagcggctt ctcgtcggcg tcggtcgcga cgacgcactg gcggaagaag tactggctgg
     4501 gggtgtgcgc gatctggatc tcggagccgg ggccgaactt ctcccacgcc tcgtcgagcc
     4561 gccagagata ccacggcagc catccgcacg tgccctcgag gaacgcgcag cgcagccccg
     4621 ggtgccgctc gaggacgccg ccggcggcca tgctgccgaa ggccatcatg agctcgaccg
     4681 ggttgcggcc ggccaccccg atgacgcggc cgttcggggt gtcgaggtag cggcgggcga
     4741 tgtcgtcgcg cagcgaggcg tgcccggtgg ggtggaagcc caccggcacc cccagctcct
     4801 cgatggcggc ccacagcggc tccatctccg ggtcgtggac gtggcggccg ttgatcgggt
     4861 tcgggttgcc gacgaccgcc accgcgccga gctggcgcac cgcgcggcgg gcctccgcga
     4921 tcgcgccggg cacgtcgtgg aacgccacct gcgccgcgaa cttcaggcgc gacggcgcgt
     4981 gctggcagta gtcgtgggtc cagtcgttgt gcgcccgggc cagcgcgctc gccaccgccg
     5041 ggtcgaggtc gtcgtgcatg agcacctggc gcccgcgcgt gccgtagagg acggccccgt
     5101 cgatgccctc gatgttcatg gcctggatgt gcgtctccgc gtcgtagccg cgcgcgtgcg
     5161 cgatctcgta gtgcggcgac cgcgcgaagg cgcgttgctg gaggtcccgg ccgaccaccg
     5221 cggtgcgggc gcgcttggac atctcgccca gctcgagatc gccgatctgg atgaccggtt
     5281 gattcggcga gcgctcgctc gagcgcagga accgaggggc gaacttcttg aacggctcgg
     5341 cgaggtagcg ctcccagagc ccgtcgggct ccatcgtgtg caggtccgag tccatgacct
     5401 tgaagccgtt cttcatcgcg ggcctcctcg tggcgggcgg atcgctcggt gcgcccgagg
     5461 atctgcccgg agaggccgcg gcgtcaaggg gcatgctacc ctgcggcccc atgaagctcg
     5521 gcacctcggt ccgcttcctc ttcccgacgt cgccggccac ccacgagcga ttccggagcc
     5581 tcgtcgcgtc gatgccgaag ggcgccttca tcgagcggcc gctgggcgcc tacgcggtgg
     5641 acgagcaggc gcgcaacctg atggagatcg cggccgcggc ccgtgcggcc gggctggacg
     5701 ggctgctgac cggtgacagc
//
LOCUS       DATMCV010000793         5728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_642057, whole genome shotgun sequence.
ACCESSION   DATMCV010000793 DATMCV010000000
VERSION     DATMCV010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642057"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VNU03_18170"
     CDS             <1..886
                     /locus_tag="VNU03_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76451.1"
                     /translation="QGRSAEMLEVTQTVLPALADDGYTWGYHAFAPEENHRFDEALPL
                     AERALARNPRDAWAVHAIAHVHYERGDNRRGIESLPGQIHPCDHLGYFRNHLLWHLAL
                     LHLAEGDYERGGRLFESVFGRIPIAIASDLQDSVALAWRLDLFGRPDPRRWSQLGEAA
                     RRWLEMPLLLFHDLHVGMALAAAGDWSGATLQLDRLRQRATKSKNPTLGEVVVPLIEG
                     LHAFARGEYGAAVARIEPVEGRIVEVGGSHAQREVFHDTLLAAALRGSEPRAVALLER
                     RLAKRPNPGRFWERARAS"
     gene            883..2211
                     /locus_tag="VNU03_18175"
     CDS             883..2211
                     /locus_tag="VNU03_18175"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP76452.1"
                     /translation="MNWEALGRETVELLRQYLMLDTTNPPGNETLGARFLEDVLGRDG
                     IASETAESAPGRGNLVARLRGDGSEGGIVLHHHIDVVYADRRYWTVDPFGGAIQDGFL
                     YGRGALDMKSTGILHLAGILAIKRAGVPLKRDLVLLATADEEAGSRFGAQWVADRHRE
                     WLADAEYALSELGGIYPAEGHRAPFASIVTSEKTGLPLRLTARSEPGHGSMPWPDTAP
                     NRLIRALGRLLEAERPLRVLPEIQEYFERLGSVMAGDAGTGYEDLARSLEDPTFRRAF
                     LADRHQAAMLRTTFAVTMLRGSEKRNVIPPEAIADVDCRMLRGDDPEEIVAWVHGAIA
                     DPRVEVSLINPPPKIPNASPPDTALYKALGEALRRRAPGAVVAPDILVGFTDNWVFRN
                     CGLHGYGFSPFILEESEWRRVHGNDERISLENLREGVRCHTEMLLDMAAA"
     gene            2211..2696
                     /locus_tag="VNU03_18180"
     CDS             2211..2696
                     /locus_tag="VNU03_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP76453.1"
                     /translation="MARASLVIRAGTARDVPTILALIRGLAEYERLDHQMEATPARVR
                     AHGFGPRRYFQTLICRRGGRPIGFALYFFTYSTFLARPSLYLEDLFVLPEERGTGAGK
                     ALLRALARIAVRRGCGRMEWAVLDWNRPAIRFYRRLGAQLRRQWILTRLTGTPLRRLA
                     R"
     gene            2707..3219
                     /locus_tag="VNU03_18185"
     CDS             2707..3219
                     /locus_tag="VNU03_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76454.1"
                     /translation="MAHLVVAVVSLGLLLSLAGPAAAQPISCPDGTRLGSSESPAGKL
                     QWCERADPRGSIRHGPMVGSYPNGNRRLELHFVDGTPRGPIRAWYESGQASAIGETRP
                     DNGTLTLRDENGRKRAQIDVRDRQLVTQAWDANGKEERYEEAKLAKSLAANRELALIM
                     NLFAVGIGIQ"
     gene            complement(3233..4666)
                     /locus_tag="VNU03_18190"
     CDS             complement(3233..4666)
                     /locus_tag="VNU03_18190"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP76455.1"
                     /translation="MTDRDLCFVPAATLLRLYRTRKVSPLEVMRAVLARIDAVNPRVN
                     AYVTVAREAALAAAKKATAALGRKGAALGPLRGIPVSIKDLTPTKGIRTTWGSKVFEH
                     HVPDEDALYVARLKDAGAIVVGKTNTPEFGAGGNTFNAVFGATRNPWNPALTCGGSSG
                     GAAVALATGMGPLAQGSDLGGSLRTPAAFCGVVGFRTTPGLIPVYPADIGWDSLSVTG
                     PMARTVGDVALMLSAMAGPDDRAPLSYDVDPRRFPAAVKQPSVKGWRAAWTRDLNGLI
                     PVDDEVAAVAERATSVFRSLGVRVEEACPDFEEVNDIVLASRGLSMVARHADKLAEWE
                     PVMQKGLIWNIKQGLALTSEQIGRGEVLRTQLWHRVREFMATRDLLLLPTVAMPPFPV
                     EQPYPTEINGKPLDNYTQWFFLTYGITVTGLPVISVPCGFTRSGLPVGLQIVGRRRQE
                     AAVLRAAAAFEAAAPWAAAIPPVVAGG"
     gene            4744..>5728
                     /locus_tag="VNU03_18195"
     CDS             4744..>5728
                     /locus_tag="VNU03_18195"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76456.1"
                     /translation="MDDLVIRGGEVLDGDGAAPVRADVAIRDGRIVSLAAPYAGTARR
                     TIDARGLVVAPGFIDIKTHSDFTLPYAPGAESKVFQGVTTEVIGHYGLSVAPAVPGRA
                     GLLQEYLAGFAPWIEVRETSFARYMDRFPATAVNVIMQVGHNTLRLMTVGLDDRAPTD
                     EERAAMGRLLAEGLAAGAIGLSSGLFTAPGSFARPDELHELLAVVRRHGGTYATHVRD
                     EAGGVFEAVRETIAAAEATGVHVQIVHLKLSGTDNWGRAGELIAEIEAARARGVRVDC
                     DAYPYTFAANPLRNLLPLWVQEGGLQAMLGRLGDPDVRRRIGREIEERGLTS"
BASE COUNT          789 a         2075 c         2093 g          771 t
ORIGIN      
        1 gcagggccgc tcggccgaga tgctcgaggt gacgcaaacc gtgctgccgg ccctcgccga
       61 cgacgggtat acgtggggct accacgcctt cgcccccgag gagaaccacc gcttcgacga
      121 ggcgctgccg ctcgcggagc gggcgctcgc gcggaacccg cgggacgcgt gggcggtgca
      181 cgccatcgcc cacgtccact acgagcgggg cgacaaccgc cgcggcatcg agagcttgcc
      241 ggggcagatc catccgtgcg accacctggg ctacttccgc aaccacctgc tctggcacct
      301 cgcgctgctc cacctggccg agggcgacta cgagcgaggc gggcggctct tcgagagcgt
      361 gttcgggcgg atcccgatcg cgatcgcctc cgatcttcaa gactcggtgg cgctcgcgtg
      421 gcgcctcgac ctcttcgggc ggcccgatcc gcggcgctgg tcccagctcg gggaggccgc
      481 gcggcgctgg ctcgagatgc cgctcctgct cttccacgac ctgcacgtgg ggatggccct
      541 cgccgccgcg ggcgactgga gcggcgcgac gctccagctc gaccggctcc ggcagcgcgc
      601 gaccaagtcg aagaacccca ccctcggcga ggtcgtggtg ccgctgatag aggggctcca
      661 cgccttcgcg cggggcgagt acggggccgc ggtggcccgc atcgagcccg tggagggtcg
      721 catcgtcgag gtgggcggca gccacgccca gcgcgaggtc ttccacgaca cgctgctggc
      781 cgcggccctg cgcggctccg agccccgcgc ggtcgccctg ctcgagcgcc gcctggccaa
      841 gcggcccaac cccggccgct tctgggagcg ggcgcgcgcg tcatgaactg ggaggccctc
      901 gggcgcgaga ccgtcgagct gctccggcag tacctgatgc tcgacaccac caacccgccc
      961 ggcaacgaga cgctcggcgc gcgcttcctc gaggacgtgc tggggcgcga cggcatcgcg
     1021 agcgagaccg cggagtccgc gcccggccgc ggcaacctgg tggcgcgcct gcggggcgac
     1081 gggtccgagg gcggcatcgt gctccatcat cacatcgacg tcgtctacgc ggaccggcgc
     1141 tactggacgg tggacccgtt cggcggcgcg atccaggacg gcttcctcta cggccgcggc
     1201 gcgctcgaca tgaagtcgac cgggatcctc catctcgccg ggatcctggc catcaaacgg
     1261 gccggggtgc cgctcaagcg cgatctggtc ctgctcgcga ccgcggacga ggaggcgggc
     1321 agccgcttcg gcgcccagtg ggtggccgac cgacaccggg agtggctcgc cgacgcggag
     1381 tacgcgctct ccgagctggg cggcatctat cccgccgaag ggcaccgggc gcccttcgcg
     1441 agcatcgtca cctccgagaa gacgggtctc ccgctccggc tcaccgcgcg cagcgagccc
     1501 gggcacggct cgatgccgtg gcccgacacc gcgccgaacc ggctcatccg cgcgctcggc
     1561 cggctgctcg aggcggagcg gccgctccgc gtgctgccgg agatccagga atacttcgag
     1621 cggctcggct cggtgatggc gggcgacgcg ggcacgggct acgaggatct cgcccgatcg
     1681 ctcgaggacc cgaccttccg tcgcgcgttc ctggccgacc ggcatcaggc cgcgatgctg
     1741 cggaccacgt tcgcggtcac catgctgcgg ggcagcgaga agcgcaacgt gatcccgccc
     1801 gaggcgatcg ccgacgtcga ctgccggatg ctgcggggcg acgatccgga ggagatcgtc
     1861 gcctgggtgc acggcgccat cgcggacccg cgcgtggagg tgagcctgat caacccgccg
     1921 ccgaagattc ccaacgcctc gccgcccgat accgcgttgt acaaggcgct cggcgaggcg
     1981 ctccgacgcc gcgccccggg ggcggtggtc gcgcccgaca tcctggtcgg cttcaccgac
     2041 aactgggtct ttcgcaactg cgggctgcac ggctacggct tcagcccctt catcctggag
     2101 gagagcgagt ggcgtcgcgt ccacggcaac gacgagcgga tctcgctcga gaacctgcgc
     2161 gaaggcgtgc ggtgtcacac cgagatgctg ctcgacatgg cggcggcctg atggcacgcg
     2221 cgtccctcgt catccgggcc ggcaccgcgc gggacgtgcc gaccatcctg gccctcatcc
     2281 gcggcctcgc cgagtacgag cggctcgacc accagatgga ggcgacgccg gcgcgggtgc
     2341 gcgcccacgg cttcggcccg cgccgctact tccagaccct catctgccgg cgcggcggcc
     2401 gtccgatcgg cttcgcgctc tacttcttca cgtactccac gttcctcgcg cggccgagtc
     2461 tctacctgga agacctcttc gtgctgccgg aggagcgggg cacgggcgcc gggaaggcgc
     2521 tgctccgcgc gctggcccgc atcgcggtcc ggcgcgggtg cgggcggatg gagtgggcgg
     2581 tgctcgactg gaaccgtccc gcgattcgct tctaccggcg cctgggggcc cagctcaggc
     2641 gacagtggat cctcacccgc ctcaccggga ctccgctgcg ccgactggcg cgctgaccgg
     2701 gcgccggtgg cccacctcgt cgtcgcggtc gtgtcgctgg gcctgctgct ctcgctggcc
     2761 gggccggccg ccgcgcagcc catctcgtgc cccgatggca cgaggctcgg cagcagcgag
     2821 agtccggccg ggaagctcca gtggtgcgag cgcgccgacc cccgcggatc gatccggcac
     2881 gggcccatgg tgggttccta tccgaacggg aaccgccggc tcgagctgca cttcgtggac
     2941 ggcaccccgc gtggcccgat ccgggcgtgg tacgagagcg ggcaggccag cgcgatcggc
     3001 gagacgcggc ccgacaacgg cacgctgacg ctgcgcgacg agaacgggcg caagcgcgcc
     3061 cagatcgacg tgcgggatcg gcagctggtc acccaggcgt gggacgcgaa cggtaaagag
     3121 gagcgctacg aggaggcgaa gctcgccaag tcgctcgcgg ccaaccgcga gctggccctc
     3181 atcatgaacc tgttcgcggt gggcatcggc atccagtgag cggtcgccgc gatcacccgc
     3241 cggccaccac cggtgggatc gcggcggccc acggcgcggc ggcctcgaag gcggcggcgg
     3301 cccgcagcac cgcggcctcc tggcgccgcc ggccgacgat ctgcagcccc accggcagcc
     3361 cgctgcgggt gaagccgcag gggaccgaga tgacgggcag accggtcacc gtgatgccgt
     3421 aggtcaggaa gaaccactgg gtgtagttgt cgagcggctt gccgttgatc tcggtgggat
     3481 agggctgctc gaccggaaac ggcggcatcg cgacggtggg caggagcagg aggtcgcggg
     3541 tggccatgaa ctcgcgcacg cggtgccaga gctgggtgcg caggacctcg ccccggccga
     3601 tctgctccga ggtcaacgcg agtccctgct tgatgttcca gatcaggccc ttctgcatga
     3661 ccggctccca ctcggcgagc ttgtcggcgt ggcgcgcgac catgctgagc ccgcggctcg
     3721 ccagcacgat gtcgttgacc tcctcgaagt cgggacacgc ctcctcgacg cgaaccccga
     3781 gggagcggaa caccgacgtg gcccgctcgg ccaccgcggc cacctcgtcg tccaccggga
     3841 tcaggccgtt caggtcccgt gtccacgccg cgcgccagcc cttgaccgac ggctgcttca
     3901 ccgcggccgg gaagcgccgc gggtcgacgt cgtaggacag cggcgcgcgg tcgtcgggac
     3961 ccgccatcgc ggagagcatg agggccacgt cgccgacggt gcgggccatg ggcccggtca
     4021 cgctcagcga gtcccacccg atgtcggcgg gatagaccgg gatcaggccc ggcgtggtcc
     4081 ggaagccgac caccccgcag aaggcggcgg gcgtgcgcag cgagccgccc aggtccgagc
     4141 cctgggcgag cggccccatg ccggtagcga gggccaccgc ggcgccgccg ctcgagccgc
     4201 cgcaggtgag cgcggggttc cacgggttgc gggtagcccc gaagaccgcg ttgaaggtgt
     4261 taccgccggc cccgaactcc ggcgtgttgg tcttgcccac cacgatggcc cccgcgtcct
     4321 tgagccgcgc cacgtagagc gcgtcctcgt ccggcacgtg gtgctcgaag accttcgagc
     4381 cccacgtggt gcggatgccc ttggtggggg tcaggtcctt gatcgagacc gggatgccgc
     4441 gcagcggccc cagcgcggcg cccttgcgcc cgagcgcggc ggtcgccttc ttcgcggcgg
     4501 cgagcgccgc ctcgcgggcc accgtgacgt aggcgttcac gcgcgggttg accgcgtcga
     4561 tgcgggccag gaccgcccgc atcacctcga gcggggagac cttccgggtc cggtagaggc
     4621 gcagcagggt ggcggcgggc acgaagcaga ggtcacggtc ggtcacggcg ggtctccctt
     4681 ctttgctcgc tacctaacta gcacacttgg aaggaacgat ggactactat ccgggagctc
     4741 gacatggacg atctcgtcat ccgcggcggg gaggtgctgg acggcgacgg ggcggccccg
     4801 gtgcgcgcgg acgtcgcgat ccgcgacggc cggatcgtct cgctcgccgc gccctacgcg
     4861 gggacggcgc gccgcacgat cgacgcccgc ggcctggtgg tggcgcccgg gttcatcgac
     4921 atcaagaccc actcggactt cacgctgccc tacgcgcccg gggccgagag caaggtgttc
     4981 cagggcgtga cgaccgaggt gatcggccac tacgggctct ccgtcgcgcc cgcggtgccc
     5041 gggcgcgccg gcctgctcca ggagtacctg gccgggttcg ctccctggat cgaggtccgg
     5101 gagacgagct tcgcccggta catggaccgc ttcccggcca ccgcggtgaa cgtgatcatg
     5161 caggtcggcc acaacacgct gcgcctgatg acggtcgggc tggacgaccg cgcgccgacc
     5221 gacgaggagc gggccgcgat gggacggctg ctcgccgagg gcctggcggc cggcgcgatc
     5281 ggcctgtcct ccggcctctt caccgcgccg ggctccttcg cccgcccgga cgagctgcac
     5341 gagctgctcg cggtggtccg gcgtcacggc ggaacgtatg ccacccacgt ccgtgacgag
     5401 gcgggaggcg tcttcgaggc cgtccgcgag acgatcgcgg cggccgaggc gaccggggtc
     5461 cacgtgcaga tcgtccacct gaagctctcg ggcaccgaca actgggggcg cgccggcgag
     5521 ctgattgcgg agatcgaggc cgcgcgggcg cggggcgtgc gggtcgattg cgacgcctat
     5581 ccgtacacct tcgcggccaa cccgcttcgc aacctgcttc ccctgtgggt gcaggaaggc
     5641 ggcctgcagg ccatgctcgg gcgcctcggc gatccggacg tcagacggcg catcgggcgc
     5701 gagatcgagg agcgcgggct caccagct
//
LOCUS       DATMCV010000794         5813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_109355, whole genome shotgun sequence.
ACCESSION   DATMCV010000794 DATMCV010000000
VERSION     DATMCV010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109355"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="VNU03_18200"
     CDS             <1..1737
                     /locus_tag="VNU03_18200"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HWP76457.1"
                     /translation="RLAQTFADQAAVALENARLFEEAERRRWQSLALGAVSQTLAESL
                     HPTGVAQRVVDAARDLLGVRVATLYHLDPITGALEESACAGRLPGREWSLRHPRGTGA
                     VGLAAAERRAVVSNDVLLDPRLTFAPEMRAHIEGTGLRAVMAVPLTVKEQVVGALGLV
                     DAAGRVFNVEDVQLARALADQASVALENARLYADAEQRRLEGETLSGLVRTISATLDL
                     AAVMRHVAAAARELCRSDIAHILLRDAESDTMSTQYGVGHMRAYGARIERGRGAGGTA
                     WSTGRPFRSANRATDSRIVDHYPEQVAREGVVTTLVVPIWISGQVEGLLYVENRTDRV
                     FTDRDEAILLRLADHAAVAIQNATLYQRLQGLSGRLMEVQETERRHLARELHDEIGQL
                     LTGLRLTLDLPEPPSPALGERLEQARGLVEELLDRVRALSLDLRPSILDDLGLLPALL
                     GHVERYTSQTKVQVRLEHHGLDRRFDPETETGVYRIVQEALTNVARHSGVDEVTVRLW
                     GTRDVLGVQVEDHGRGFDVDAVLGNGGGLAGLRERAVLLNGHLTVETKPGGGVRLTAE
                     VPVNGSPKGDGE"
     gene            1734..2387
                     /locus_tag="VNU03_18205"
     CDS             1734..2387
                     /locus_tag="VNU03_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWP76458.1"
                     /translation="MSISIVLAEDHHVVRQGLRALLAAETDFVVVGEAASGLEVAPLV
                     ERVKPDVLVVDFVMPGLNAPDIVRRATRLSPRTRALVLSMHANPAYVVEAIQSGAAGY
                     VLKGSSAAELVRAIRAVASGERHLSPPLSEEMVEAYAQRAVPTGLDLYRTLTQREREV
                     LHLAAEGQTSAQIGARLNISPRTVEVHRANLMRKLGLRGQGDLVRYALGRGILTVDG"
     gene            2480..2878
                     /locus_tag="VNU03_18210"
     CDS             2480..2878
                     /locus_tag="VNU03_18210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP76459.1"
                     /translation="MADPNSPATILPATILIVEDEEDVRELTRDVLEMNGFTVLESLD
                     PADAIRIAETHPGSIDLLITDVVMPRMSGPELARRLRETRPALRVLCMSGYPESAERG
                     AAGASQWTAWLQKPFNPDGLILKVRECLTS"
     gene            complement(2868..3119)
                     /locus_tag="VNU03_18215"
     CDS             complement(2868..3119)
                     /locus_tag="VNU03_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76460.1"
                     /translation="MYHPPIFFDPQFTFGVMAGWVLTLAGVGALLLAGVWYSFAGEWR
                     RGTPPPGAFRALLVLGALAWAGGLLWQFVGYFGTGTLSW"
     gene            complement(3134..3901)
                     /locus_tag="VNU03_18220"
     CDS             complement(3134..3901)
                     /locus_tag="VNU03_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HWP76461.1"
                     /translation="MQIAGSTTVITGGASGLGRATAERLHAAGGSVVLLDLARSPGKE
                     VAAGLGDRALFAPADVTSPDEVGAALEAAVGRFGGVQGLVNCAGIGTAEKTFGKRGSA
                     DLTAFSRTIQVNLIGTFNCIRLAAAQMAKNAPNGEGERGVIINTASVAAFDGQIGQAA
                     YSASKGGVVGMTLPIARDLAELGIRVCTIAPGLFETPMLAGLPEPARVSLGKQVPFPP
                     RLGRPTEYAALAAHIIENPMLNGETIRLDGAIRMQPR"
     gene            3956..5092
                     /locus_tag="VNU03_18225"
     CDS             3956..5092
                     /locus_tag="VNU03_18225"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HWP76462.1"
                     /translation="MTPPLWIRTADDLAALAAELAGTPALAIDTEADSLHHYPGKLCL
                     VQVADAEGRGHLVDPLVLRDLSPLGPVLADPGTIKVLHAADNDLSYLKRLYGVTVTSL
                     FDTAVAARFIGATSLGLEGLLTQYLGVTAVKSRQKDDWSRRPLTGPQEAYALDDVLHL
                     IPLRARLLEELRAIGREQWVEEECAALAALEVPEKAADPDAYMKLKGARELDARGLAV
                     LRELHAARETLALRLDRPPFMIVGNESLVALAKRLPADNEAILSVAGCTPPVVRRAGP
                     AILEAVARGLALPTDELPVYRPMPRPHLPAAVRRRAEALRAWRTRASKEIGLEPGVLF
                     PQRLIDRLAAAPPTDLAGLRQVEGVHGWRVGLLGPAVLEALAAA"
     gene            5208..>5813
                     /locus_tag="VNU03_18230"
     CDS             5208..>5813
                     /locus_tag="VNU03_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76463.1"
                     /translation="MASHSRYLREGVIAGLIGAALVAVWFLIYDAASGRPFRTPALLG
                     AATFEGVTNPSTVPTAAHLVVPYTVLHGVVFAMIGVLIAYLIVSAQREPSRVLMLFIA
                     LMSFEIAFLAMVTWLAHPVLDELAWWAILVANALAAAGMLIYLLVGHRAVGRALLGPL
                     WSRAVREGIWAGLLGAAVVAVWFLAYDAAAGVPLRTPALLGA"
BASE COUNT          759 a         2108 c         2150 g          796 t
ORIGIN      
        1 cgcctggccc agaccttcgc ggaccaggcc gcggtggcgc tcgagaacgc ccggctcttc
       61 gaggaggccg agcggaggcg atggcagagc ctcgccctgg gcgcggtgag ccagaccctc
      121 gccgagtcgc tccatccgac cggggtggcc cagcgagtgg tggacgcggc gcgcgacctg
      181 ctcggcgtgc gggtcgcgac cctctatcac ctcgacccga tcaccggagc gctcgaggag
      241 agcgcgtgcg ccggccgcct gcccggccgc gagtggtcgt tgcggcatcc gcgcggcacc
      301 ggggcggtcg ggctcgccgc cgcggagcga cgggccgtcg tgtccaacga cgtgctgctc
      361 gacccgcggc tcacgttcgc cccggagatg cgcgcccaca tcgaggggac cgggctccgc
      421 gcggtgatgg cggtgccgct gaccgtgaag gagcaggtgg tgggcgcgct cggcctggtc
      481 gacgcggcgg ggcgggtctt caacgtggag gacgtgcagc tggcccgggc cctggccgat
      541 caggcgtcgg tggcgctcga gaacgcccga ctctacgccg acgccgagca gcggcggctc
      601 gagggcgaga ccctctcggg cctggtccgc accatcagcg ccaccctcga cctggccgcg
      661 gtcatgcgcc acgtggccgc ggcggcccgc gagctctgtc gcagcgacat cgcgcacatc
      721 ctgctccgtg acgccgagag cgacaccatg tccacccagt acggggtggg tcacatgcgc
      781 gcctacgggg cgcgcatcga gcgcgggcgc ggcgcgggcg ggaccgcgtg gagcaccggg
      841 cggccgttcc gcagcgcgaa ccgcgcgacg gactcgcgaa tcgtcgacca ctacccggag
      901 caggtggcgc gagagggcgt cgtcaccacg ctggtggtgc cgatctggat cagcggccag
      961 gtcgaggggc tgctctacgt ggagaatcgg accgaccgcg tcttcaccga tcgcgacgag
     1021 gcgatcctgc tgcgcctcgc cgaccacgcg gcggtggcga tccagaacgc caccctctac
     1081 cagcggctcc agggtctctc cgggcgcctg atggaggtgc aggagaccga gcgccggcac
     1141 ctcgcgcgcg agctgcacga cgagatcggc cagctcctca ccggcctgcg cttgacgctc
     1201 gacctgcccg agccgccgtc ccccgcgctg ggcgagcggc tcgagcaggc ccgcggcctg
     1261 gtggaggagc tgctcgaccg ggtgcgcgcg ctgtcgctcg acctgcgccc gagcatcctc
     1321 gacgacctgg ggctcctgcc ggccctcctc gggcacgtcg agcgctacac gagccagacc
     1381 aaggtgcagg tgcgcctcga gcaccacggt ctcgaccgac gcttcgaccc ggagaccgag
     1441 accggcgtct accgcatcgt gcaggaggcc ctcaccaacg tggctcggca cagcggggtg
     1501 gacgaggtga cggtgcggct ctggggcacc cgcgacgtcc tgggggtgca ggtcgaggac
     1561 cacgggcgcg gattcgacgt cgacgcggtg ctgggcaacg gcggaggtct ggccggcctt
     1621 cgcgagcgcg cggtcctgct caacgggcac ctgacggtgg agacgaagcc cggcggaggc
     1681 gtgcgcctca ccgcggaggt cccggtcaac ggctccccca agggggacgg agaatgagca
     1741 tctcgatcgt cctggccgag gaccaccacg tggtccggca ggggctgcgg gccctgctcg
     1801 cggccgagac cgacttcgtg gtggtggggg aagcggcgag cggcctcgag gtggcgccgc
     1861 tggtcgagcg cgtgaagccc gacgtgctcg tggtcgactt cgtcatgccg gggctcaacg
     1921 cgcccgacat cgtgcgccgg gccacgcgcc tgtcgccgcg cacccgagcc ctcgtgctgt
     1981 cgatgcacgc caacccggcc tacgtggtgg aggcgatcca gagcggggcg gccggctacg
     2041 tgctcaaggg ctcgagcgcg gcggagctgg tgcgggccat ccgcgcggtg gcgagcgggg
     2101 agcggcacct gagcccgccg ctctccgagg agatggtcga ggcctacgcg cagcgcgcgg
     2161 tgccgaccgg cctcgatctc tatcgcaccc tgacccagcg cgagcgcgag gttctgcacc
     2221 tggccgcgga gggccagacc agcgcccaga tcggcgcgcg gctcaacatc agcccgcgca
     2281 cggtggaggt gcaccgggcc aacctgatgc gcaagctcgg actccgcggg cagggcgacc
     2341 tggtgcgcta cgcgctcggg cgcggcatcc tcaccgtcga cggctgatcc aaaatacgta
     2401 agcgtacgaa tagacgggcg cggtgcgttg cgtgcatcgt cgcctgcagt gctgaccgag
     2461 gcctcgcggc ggcatctccg tggctgaccc gaattccccc gcgacgatcc tgcccgcgac
     2521 gatcctgatt gtcgaggacg aggaggacgt gcgagagctg acgcgcgacg tcctcgagat
     2581 gaacggcttc acggtcctgg agtcgctgga ccccgccgac gccatccgga tcgccgagac
     2641 ccatcccggg tcgatcgacc tgctgatcac cgacgtggtc atgccgcgca tgagcggtcc
     2701 cgagctggcg cggcgcctcc gggagacccg gcccgcgctg cgcgtgctct gcatgtcggg
     2761 ttacccggag agcgcggagc gcggggcggc cggcgcgtct cagtggaccg cgtggctgca
     2821 gaagccgttc aaccccgacg gcctgatctt gaaggtgcgc gagtgcctta ccagctgagg
     2881 gtgccggtcc cgaagtagcc gacgaactgc cagagcagcc cgcccgccca ggccagcgcg
     2941 cccagcacca gcagggcccg gaacgcgccc ggcggcggcg tgccgcgccg ccactcgccg
     3001 gcaaaggagt accacacccc ggcgagcagc agggcgccga cgccggccag ggtcagcacc
     3061 cagcccgcca tcacgccgaa ggtgaactgc gggtcgaaga agatcggggg atggtacatg
     3121 gtcgctcctg tcctcagcgt ggctgcatgc ggatggctcc gtcgagccgt atggtctcgc
     3181 cgttgagcat cgggttctcg atgatgtgcg cggcgagggc cgcgtactcg gtcggacggc
     3241 cgagccgcgg cgggaagggg acctgcttgc cgagcgagac gcgcgcgggc tcgggcaggc
     3301 cggctagcat cggcgtctcg aagagcccgg gcgcgatcgt gcacacgcgg atgccgagct
     3361 cggccaggtc gcgggcgatc ggcagggtca tgcccacgac gccgcccttg gacgcggagt
     3421 aggcggcctg gccgatctgg ccgtcgaagg ccgccaccga ggcggtgttg atgatcacgc
     3481 cccgctcacc ctctccgttg ggggcgttct tggccatctg ggccgcggcc agccggatgc
     3541 agttgaacgt gccgatcagg ttcacctgga tggtgcggga aaaggcggtc agatccgccg
     3601 agccgcgctt gccgaaggtc ttctcggcgg tgccgatgcc cgcgcagttg accagaccct
     3661 gcacgccccc gaagcgcccc accgccgcct ccagggccgc gccgacctcg tcggggctgg
     3721 tcacgtcggc gggagcgaag agggcccgat cgccgaggcc ggccgccacc tccttgccgg
     3781 gcgatcgcgc gaggtccagc agcaccacgg agccgcccgc cgcgtggagc cgctccgcgg
     3841 tggcccggcc gaggccggag gctccacccg tgatgaccgt cgtcgagccc gcgatctgca
     3901 ttgccgtcct cctcccaggg gagtgtcacc gacggtgtag tataaccgag cgcccatgac
     3961 cccccccctc tggatccgga ccgccgacga cctcgccgcg ctggccgcgg agctcgcggg
     4021 cacgccggcg ctggcgatcg acaccgaggc cgacagcctg caccactacc cgggcaagct
     4081 ctgcctcgtc caggtggcgg atgccgaggg gcgggggcac ctcgtggatc ccctggtgct
     4141 ccgcgacctc tccccgctcg gcccggtgct ggccgatccc gggacgatca aggtgctgca
     4201 cgcggccgac aacgacctgt cctatctcaa gcggctgtac ggcgtgacgg tgacctccct
     4261 gttcgacacc gcggtggccg cgcgcttcat cggcgcgacc tcgctcggtc tcgaagggct
     4321 gctgacccag tacctcggcg tgaccgcggt caagtcgcgc cagaaggacg actggtcgcg
     4381 gcgccccctg accgggcccc aggaggccta cgcgctcgac gacgtgctcc acctgatccc
     4441 gctccgggcg cggctgctcg aggagctgcg cgcgatcggc cgcgagcagt gggtggagga
     4501 ggagtgcgcg gcgctggccg cgctcgaggt ccccgagaag gcggcggatc cggacgccta
     4561 catgaagctc aagggggcgc gcgagctgga cgcgcgcggg ctcgcggtgc tgcgcgagct
     4621 gcacgccgcg cgcgagaccc tcgcgctccg cctggatcgt ccgccgttca tgatcgtcgg
     4681 caacgagagc ctggtggcgc tcgccaagcg cctgccggcc gacaacgagg ccatcctgtc
     4741 ggtggcgggc tgcaccccgc cggtggtgcg ccgggccggt ccggccatcc tggaggcggt
     4801 ggcccgcggg ctcgcgcttc ccaccgacga gctgccggtc taccggccca tgccgcggcc
     4861 ccatctgccc gccgcggtgc gccggcgagc cgaggccctg cgggcctggc gcacccgcgc
     4921 ctccaaggag atcggtctcg agcccggggt cctgtttccc cagcggctca tcgaccgcct
     4981 cgccgccgcc ccgccgaccg acctggccgg gctccgccag gtcgagggcg tgcacggctg
     5041 gcgcgtgggt ctgctgggcc ccgcggtcct ggaggccctg gcggcggcct gacccggccc
     5101 ggctcccgcg gccggccgcg gcgccctgcg gcttgccgtg ggtggccgat ggtggtacgc
     5161 ctgagcctac gtcgtctgac cggactggtt cggcaaggag ggtccccatg gcctcccatt
     5221 cccggtatct ccgcgaaggc gtcatcgccg gcctcatcgg ggccgccctg gtcgccgtgt
     5281 ggttcctcat ctatgacgcc gccagcgggc ggccgttccg cacccccgcg ctgctgggcg
     5341 cggccacctt cgagggggtg acgaatccct cgacggtgcc gaccgcggcg cacctcgtcg
     5401 tgccgtacac cgtgctgcac ggcgtggtgt tcgcgatgat cggggtgctg atcgcgtacc
     5461 tgatcgtctc ggcccagcgc gagccgagcc gggtgctgat gctgttcatt gcgctcatgt
     5521 cgttcgagat cgccttcctg gcgatggtga cctggctcgc gcaccccgtg ctcgacgagc
     5581 tcgcgtggtg ggccatcctg gtcgccaacg cgctcgccgc ggccgggatg ctgatctacc
     5641 tgctggtggg ccaccgcgcg gtcggccgcg cgctgctcgg tccgctctgg agccgtgcgg
     5701 tgcgcgaggg gatctgggcc ggcctgctgg gcgccgcggt ggtcgcggtc tggttcctgg
     5761 cctacgacgc cgcggcgggg gtgccgctgc gcacgccggc cctgctcggg gcc
//
LOCUS       DATMCV010000795         4113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_838829, whole genome shotgun sequence.
ACCESSION   DATMCV010000795 DATMCV010000000
VERSION     DATMCV010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838829"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VNU03_18235"
     CDS             complement(<1..500)
                     /locus_tag="VNU03_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76464.1"
                     /translation="MNESTLYYTFSTVPQTLAGGGAILVAVVLYRLGEIDRIIDTAAD
                     HLETAWQGHPFRELWRALERNGWKGVDRVLRRLDAGEAAAGAAEQAACRRAYRAMRAR
                     RQILFLLYSALGFTVLDILVCITSIPVTPNLLKSPADASQVVVGTIVLTGICLGFYAR
                     LIWAMV"
     gene            538..1443
                     /locus_tag="VNU03_18240"
     CDS             538..1443
                     /locus_tag="VNU03_18240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWP76465.1"
                     /translation="MSAAYLVLLVANLVFSTGYAVTRVVLNDVGPATLGLARVLIGAV
                     LLLGWAAWQSSSGPRVPPRVRWTSGDRMRVALMGVVGFALAFAFGNWGLAHSTASNAA
                     LLITVEPTTLLVLSPLLLGERLTRRETVGAGLTLLGAVVVVVNGVPGLTRSLMPHWRG
                     DLVLILSGVAYAGYSLLGRDVLSRHPVSLVTAHSVVWGAVSIAPLALYEWHVGPPPAW
                     TAAAIAGTVYLGVVITALGYGVWNWCLERVGAARVAAFVNVQPLAGALLGVWWLREPV
                     TPFLVAGGLLIVAGLHLTVNGARRG"
     gene            1453..3177
                     /locus_tag="VNU03_18245"
     CDS             1453..3177
                     /locus_tag="VNU03_18245"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWP76466.1"
                     /translation="MRRVRVGLSQVNPTVGAVEANARLVIAGMERARGLGCDIVAFPE
                     LVLTGYPPEDLLFKPAFIEANLRALAEVARASRGLTTIVGYVDKRDDIFNAAAILHDG
                     VVAGTYHKQYLPNYGVFDENRYFQSGTESPVFTIGETVIAANICEDIWYPTGPTTLQA
                     LGGAELVVSINASPYHAGKGRFREKMLATRAADDLVCLAFVNTVGGQDELIFDGQSLM
                     FNEKGECVARGKAFEEDFVVADLDLDEVFRARLHDSRRRKEKLRATEAPPRRVTLPPL
                     PAPERPRLPAHEVAVLEPVEEIYQALVLGTRDYVTKNGFRHVVLGLSGGIDSSLVAAI
                     ACDALGPANVTGVTMSSQFSSKGTRGDAARLARNLGIEFLRLPITPVLGAYRRALARP
                     FKGLKEDVTEENLQARIRGNYLMALSNKFGWLVLTTGNKSEIGVGYSTLYGDMAGGFA
                     VIKDVPKTLVYQVSEHVNRRAGREVIPRSVFERAPSAELRPDQTDQDTLPPYATLDAI
                     LEAYVEGDQGVADIVARGFDAPTVKRVIAMVDRNEYKRRQGPIGIKITPRAFGKDWRL
                     PIVNRFRE"
     gene            3182..3508
                     /locus_tag="VNU03_18250"
     CDS             3182..3508
                     /locus_tag="VNU03_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HWP76467.1"
                     /translation="MLSSVALFLLAGLAEIGGGYLMWLWWREGRPWPLGLVGAAVLVL
                     YGLIPTYQAAHFGRVYAAYGGVFVVLSLLWGWAVDRMCPDRYDIAGVCLCLVGVAVMM
                     YAPRAA"
     gene            3511..4002
                     /locus_tag="VNU03_18255"
     CDS             3511..4002
                     /locus_tag="VNU03_18255"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HWP76468.1"
                     /translation="MADAGGRMDPADHLRRPWRVHALAAAEGLALRDVWEVATPLPAG
                     VPLARWVEALRTEPRGRSSGALFALRRALGRLLGLDRGGTGFVPVYQEPDEVLSRIAN
                     RTVTAFLHVSLVERHPRVAIYVRPKGAFGRSYMALIEPFRRWVIYPALLAAGSRAAKR
                     LGG"
BASE COUNT          526 a         1471 c         1489 g          627 t
ORIGIN      
        1 tggaccatcg cccagatcag ccgggcgtag aacccgagac agatgccggt gaggacgatc
       61 gtgccgacga cgacctggga ggcgtccgcg ggcgacttca ggaggttcgg ggtcaccggg
      121 atcgaggtga tgcacaccag gatgtcgagc acggtgaagc ccagcgccga gtagagcagg
      181 aagaggatct gccggcgcgc ccgcatggcc cggtacgcgc gccggcaggc cgcctgctcc
      241 gcggcacccg ccgcggcctc gccggcgtcc agccgacgca ggacgcggtc gacccccttc
      301 cacccgttgc gctcgagcgc gcgccacagc tcccggaacg gatggccctg ccacgcggtc
      361 tcgaggtgat cggccgcggt gtcgatgatc cgatcgatct ccccgagccg gtagaggacc
      421 accgcgacca ggatcgcgcc gcccccggcc agggtctggg ggacggtgct gaaggtgtag
      481 tagagcgtgc tctcgttcat gccgcgcgcg agcaggatac tacgctaagc tcacccggtg
      541 tccgccgcct acctggtcct cctggtcgcc aacctcgtct tctccaccgg ctacgcggtc
      601 acccgcgtcg tgctgaacga cgtggggccg gccacgctgg gcctcgcgcg cgtgctgatc
      661 ggcgcggtgc tgctcctcgg gtgggccgcg tggcaatcga gctcgggccc gcgcgtcccc
      721 ccgcgcgtcc ggtggacgag cggggaccgg atgcgggtcg cgctgatggg cgtggtggga
      781 ttcgcgctcg ccttcgcctt cggcaactgg ggcctcgcgc actcgaccgc cagcaacgcc
      841 gcgctgctca tcacggtcga gccgaccacg ctgctcgtgc tctccccgct gctgctgggc
      901 gagcggctga cgcgacggga gacggtcggc gccggcctca ccctgctggg cgccgtcgtg
      961 gtggtggtga acggggtgcc cgggctcacc cgctccctga tgccacactg gcgcggcgac
     1021 ctggtgctga tcctctccgg cgtcgcctac gccgggtact cgctcctggg ccgggacgtg
     1081 ctgagccgcc atccggtgtc ccttgtcacc gcccattcgg tggtctgggg cgcggtctcg
     1141 atcgcgccgc tggcgctcta cgagtggcac gtcggcccgc cgcccgcgtg gacggccgcc
     1201 gcgatcgcgg gcaccgtcta cctgggcgtg gtgatcaccg cgctcggcta cggggtctgg
     1261 aactggtgcc tggagcgggt gggcgccgcg cgcgtggccg ccttcgtcaa cgtgcagccg
     1321 ctggccggcg ccctcctcgg ggtgtggtgg ctgcgcgagc cggtgacccc cttcctggtg
     1381 gccgggggct tgctgatcgt ggccgggctg cacctgacgg tgaacggggc ccgaagaggg
     1441 tagaataccg gcatgcggcg ggtgcgggtg gggctcagcc aggtgaaccc gacggtgggt
     1501 gccgtcgagg cgaatgcccg gctggtgatt gccggcatgg aacgcgcgcg tggcctcggc
     1561 tgcgacatcg tggccttccc ggagctggtg ctcaccggct accccccgga agacctcctc
     1621 ttcaagcccg ccttcatcga ggccaacctg cgggcgctcg ccgaggtggc ccgggcgagc
     1681 cgcggtctca ccaccatcgt cggctacgtg gacaagcgcg acgacatctt caacgcggcg
     1741 gccatcctcc acgacggggt cgtggccggc acgtatcaca agcagtacct gccgaactac
     1801 ggcgtcttcg acgagaaccg ctacttccag tccggcaccg agtcgccggt gttcaccatc
     1861 ggcgagaccg tcatcgcggc caacatctgc gaggacatct ggtacccgac cgggcccacc
     1921 acgctgcagg ccctgggcgg cgcggagctg gtggtgagca tcaacgcctc gccctaccac
     1981 gcgggcaagg ggcggttccg cgagaagatg ctcgcgaccc gcgcggccga cgacctcgtg
     2041 tgcctggcct tcgtcaacac ggtgggcggc caggacgagc tgatcttcga cggccagtcg
     2101 ctcatgttca acgagaaggg tgagtgcgtg gcccgcggga aggccttcga ggaggacttc
     2161 gtggtggccg acctggacct cgacgaggtc ttccgggcgc gcctgcacga ttcccggcgg
     2221 cggaaggaga agctgcgcgc gaccgaggcg ccgccgcgcc gcgtgaccct gccgccgctg
     2281 cccgcgcccg agcggccccg gctgcccgcg cacgaggtgg cggtgctcga gccggtggag
     2341 gagatctacc aggccctcgt cctcggcacg cgcgactacg tcaccaagaa cgggttccgc
     2401 cacgtggtgc tgggcctctc gggcggcatc gattcgtccc tggtggccgc catcgcgtgc
     2461 gacgcgctgg ggccggccaa cgtcaccggg gtcaccatgt cctcgcagtt ctcctcgaag
     2521 ggcacgcggg gggacgcggc gcgcctggcc cggaacctcg gcatcgagtt cctgcgcctg
     2581 cccatcaccc cggtgctcgg cgcctaccgc cgcgcgctgg cccggccctt caaggggctg
     2641 aaggaggacg tgaccgagga aaatcttcag gcccgcatcc gcggcaacta cctcatggcg
     2701 ctctccaaca agttcggctg gctcgtgctc accaccggca acaagagcga gatcggggtc
     2761 ggctactcga cgctctacgg ggacatggcg ggcggcttcg cggtgatcaa ggacgtgccg
     2821 aagacgctcg tctaccaggt gtccgagcac gtcaaccggc gggccggccg cgaggtcatc
     2881 ccgcgctcgg tcttcgagcg ggccccgtcc gcggagctgc ggcccgacca gaccgatcag
     2941 gacacgctgc cgccctacgc gaccctcgac gcgatcctcg aggcctacgt ggagggcgac
     3001 cagggcgtgg ccgacatcgt ggcgcgcggc ttcgacgccc ccaccgtcaa gcgggtgatc
     3061 gcgatggtcg accgcaacga gtacaagcgc cgccagggcc cgatcgggat caagatcacc
     3121 ccgcgcgcct tcggcaagga ctggcgcctg ccgatcgtga accgcttccg cgagtaggcg
     3181 cctgctctcc tccgtcgccc tgttcctcct ggccggtctc gccgagatcg ggggcggcta
     3241 cctgatgtgg ctctggtggc gcgaggggcg gccgtggccg ctgggcctgg tcggcgccgc
     3301 cgtcctcgtc ctctacgggc tcatcccgac ataccaggcg gcccacttcg ggcgcgtcta
     3361 cgcggcctac ggcggcgtgt tcgtggtcct gtcgctgctc tgggggtggg ccgtcgaccg
     3421 gatgtgcccg gaccgctacg acatcgcggg cgtgtgtctc tgtctggtgg gcgtcgcggt
     3481 catgatgtac gcgccgcgcg cggcctagcg atggcggacg cgggcggccg catggatccc
     3541 gccgatcatc tgcggcgccc gtggcgcgtg cacgccctcg ccgccgcgga agggctcgcg
     3601 ctacgcgacg tgtgggaggt ggcgaccccg ctgccggcgg gcgtgccgct cgcgcgctgg
     3661 gtggaggcgc tgcggacgga accgcgcggg cggtcgtccg gagcgctctt cgcgctccga
     3721 cgggccctcg ggcgcctgct cggcctcgac cgcggcggga ccggattcgt gccggtctac
     3781 caggagccgg acgaggtcct ctcccgcatc gccaaccgca ccgtgaccgc cttcctgcac
     3841 gtctcgctgg tcgagcggca tcctcgagtg gcgatctacg tgcggcccaa gggcgcgttc
     3901 ggccgctcct acatggcgct catcgagccc ttccggcgct gggtgatcta cccggccctt
     3961 ctcgcggcgg gcagccgcgc ggcgaagcgg ctgggcggct agcgggtcgg cagggcgtcc
     4021 agcgactgta gcgtcgcgag ccggcggtag atgccgtcgg gccgcgccat cagctcgtcg
     4081 tggttgccca tctcggcgat gcggcccgcg tgg
//
LOCUS       DATMCV010000796         2082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_577831, whole genome shotgun sequence.
ACCESSION   DATMCV010000796 DATMCV010000000
VERSION     DATMCV010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577831"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="VNU03_18260"
     CDS             complement(<1..1983)
                     /locus_tag="VNU03_18260"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWP76469.1"
                     /translation="MHLTSKGFYVIVAPMDPFIELLGASPAIEAARDSIRRLLSRPFS
                     ARRPPAILLEGETGSGKGLVASVLHRAGPRAPRAFVPLNCAAIPETLLESELFGYERG
                     AFTDARRAKPGLFQTAHQGTIFLDEVGLLPEPLQAKLLTVIEERAVRRLGGTHPEPAD
                     AWIISATNADLQTAIRHRRFREDLYHRLAVLTLRLPPLRERGRDVILLAEHLLVRVCA
                     DYGLPLKTLSPEAQERLLAHLWPGNVRELSNVVERVALLTDGAVVTADVLELPTPVRE
                     DTAPAPVVPAPRSLDDALREHMRAVLLQTGWNISRTAAVLGITRNTVRARMEKLGLRP
                     DGPAPASGRRAAGSAAARLARVARPAPPVSPPPTAIRWESRRVTLLRAVLEWSDKGSA
                     LDVVLDKLTSFGGRIDELSPTTVGAVFGLEPVEDAPRRAAHAAMAVQNAAARGRDGEP
                     FTVTTAIHVGQVLVGQFAGDLRIDSEAKRIEWRMLDPLLAAARPGGIVASPAAVPFLA
                     RRFDLERREIGSSTLHYLRGREGPGLGVEGEMAGFVGRRQELDLLNSRLASARAGRGQ
                     IVGIVGEAGIGKSRLLYEFRQGLRGEPVTYIEGHCLSYGTSIPFLPVLEILRTACLIR
                     ETDGPAAVAAKLRKSLEWLGIEVEGALPYLLSFLGIK"
BASE COUNT          292 a          746 c          769 g          275 t
ORIGIN      
        1 cttgatcccg aggaagctga gcaggtaggg cagcgcgccc tccacctcga tcccgagcca
       61 ctcgaggctc ttgcggagct tggcggccac cgcggccggc ccgtcggtct cgcggatgag
      121 gcaggcggtg cggaggatct ccagcaccgg caggaacggg atcgacgtgc cgtaggagag
      181 acaatggccc tcgatgtagg tgaccggctc cccgcggagc ccctgccgga actcgtagag
      241 cagccgcgac ttgccgatgc ccgcctcccc gacgatcccc acgatctggc cgcgcccggc
      301 ccgcgccgag gccaggcggc tgttgaggag gtcaagctcc tgccggcgcc ccacgaagcc
      361 ggccatctcg ccctcgacgc ccagcccggg cccctcgcgc ccgcgcaggt agtgaagcgt
      421 gctcgacccg atctcgcgcc gctcgaggtc gaagcggcgg gcgaggaagg ggaccgccgc
      481 cgggctcgcc acgatgccgc ccgggcgggc ggccgcgagg agggggtcga gcatcctcca
      541 ctcgatccgc ttggcctccg agtcgatccg gagatcgccc gcgaactgcc cgaccaggac
      601 ctggcccacg tggatggcgg tggtcaccgt gaacggctcg ccgtcgcgac cgcgcgccgc
      661 cgcgttctgc accgccatgg ccgcgtgggc ggcgcgccgc ggggcgtcct ccaccggctc
      721 gaggccgaag accgcgccga cggtggtcgg gctcagctcg tcgatccggc ctccgaagga
      781 cgtgagcttg tcgagcacca cgtcgagcgc cgagcccttg tcgctccact cgaggaccgc
      841 gcggagcagg gtgacccgcc gggattccca gcggatcgcg gtgggcggcg gggaaaccgg
      901 cggagccgga cgcgccaccc gggcgagacg ggccgccgcg ctaccggccg cccgccgccc
      961 gctcgcgggc gccggcccgt ccgggcgcag gccgagcttc tccatgcgcg cccgcaccgt
     1021 gttgcgggtg atccccagca cggccgcggt gcgcgagatg ttccagccgg tctgcaggag
     1081 gacggcccgc atgtgctcgc gtagagcgtc gtcgagcgac cgcggcgccg ggacgaccgg
     1141 cgcgggagcc gtgtcctcgc ggaccggggt gggcagctcg agcacgtccg cggtgaccac
     1201 cgctccgtcg gtcagcagcg cgacccgctc caccacgttg ctgagctcgc gcacgttgcc
     1261 gggccacagg tgcgccagca gccgctcctg ggcctcgggc gacagggtct tcagcggcag
     1321 gccgtagtcg gcgcagacgc ggaccagcag gtgctcggcg aggaggatca cgtcgcggcc
     1381 gcgctcgcgc agcggcggca ggcggagcgt gaggacggcg agccggtggt agagatcctc
     1441 gcggaagcgg cggtggcgga tggcggtctg cagatcggcg ttggtggcgc taatgatcca
     1501 cgcgtcggcc ggctcggggt gcgtgccgcc caggcgtcgg accgcgcgct cctcgatgac
     1561 ggtcagcagc ttcgcctgga gcggctccgg cagcagcccg acctcgtcca ggaagatcgt
     1621 gccctggtgc gcggtctgga agagcccggg cttcgcgcgg cgcgcgtcgg tgaaggcgcc
     1681 gcgctcgtag ccgaacagct ccgactcgag cagcgtctcc ggaatcgccg cgcagttgag
     1741 cggcacgaag gcccggggcg cgcgcgggcc ggcccggtgg agcacgctgg ccaccagccc
     1801 cttgccgctc ccggtctcac cctccagcag aatggccggc ggcctccgcg cgctgaacgg
     1861 gcgcgagagc agccgccgta tggagtctcg ggccgcttcg atcgccgggc tcgcgccaag
     1921 cagctcgatg aacggatcca tcggcgcaac gatgacgtag aagcccttac tggtcaagtg
     1981 catggtcaag ttttcaccag tcgctgtcgg aaatattgac ctgaggagtt caacctgctg
     2041 gaagcatgta tctttatcgt ggcacccggc ttgcgatcgt gt
//
LOCUS       DATMCV010000797         2289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_978602, whole genome shotgun sequence.
ACCESSION   DATMCV010000797 DATMCV010000000
VERSION     DATMCV010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978602"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VNU03_18265"
     CDS             complement(<1..202)
                     /locus_tag="VNU03_18265"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP76470.1"
                     /translation="MIVACEERARHDAVTGRTEFRQADAEALPFAPGTFDFVAAMQVY
                     EYMPDVERGLAEAARVLKARGRL"
     gene            496..1287
                     /locus_tag="VNU03_18270"
     CDS             496..1287
                     /locus_tag="VNU03_18270"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP76471.1"
                     /translation="MATASPALVDPSRSTPDVAEQRARVRAIVAPRSGERGLDIGCGF
                     GLLSCELARDVGPTGRVMGVDSVPAMIVACEERARHDAVTGRTEFRQADAEALPFAPG
                     TFDFVTAMQVYEYMPDVERGLAEAARVLKARGRLAVLDTDWESCVWHSGDRERTARVL
                     QAWEQRFAHPHLPARLPELLRRTGFALRSVTLIPVVNVEASEQTYSPSMIDVIARFVA
                     NRGGITEDEAARWAEDVRAQAVRGEYFFSLSRYLFLAERTRPNRP"
     gene            1295..1474
                     /locus_tag="VNU03_18275"
     CDS             1295..1474
                     /locus_tag="VNU03_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76472.1"
                     /translation="MRLEVELVEEDGGLYRATAVLYPEVSVTGRTEKEALGLLLDAVA
                     KYLKSQPEKSQPGRA"
     gene            complement(1500..2144)
                     /locus_tag="VNU03_18280"
     CDS             complement(1500..2144)
                     /locus_tag="VNU03_18280"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWP76473.1"
                     /translation="MRCQDVRRALPLLVAGEMPLTEWALLETHLVECVECRGELDRQR
                     VQAAQRARIRRRSSTAAALVATAVVLVVAGAGFYIYETSLPDPYRPGTVRMPPRPPAA
                     PIAVTPVAPLPAPAISPAPPVARPIPPAPPAPVPVTPTTRKVETPVPPPARIPAAASE
                     TVPRPVRVPAAVPRPADPPAEERMPTQARPPAAAVTAGPGAEAMPTQGGSRPAR"
BASE COUNT          303 a          836 c          860 g          290 t
ORIGIN      
        1 cgagccggcc ccgggccttc agcacgcgcg cggcctcggc cagccctcgc tcgacgtccg
       61 gcatgtactc gtagacctgc atggcggcga cgaagtcgaa ggtgccgggc gcgaatggca
      121 gcgcctcggc gtccgcctgg cgaaactcgg tgcggccggt gaccgcgtcg tggcgcgcgc
      181 gctcctcgca ggccacgatc atggccggga ccgagtaggc gtcgccgggc gggagcggga
      241 gccgcggcgg gcgcggcggg gcctcccggg gacacgctga ctcgcaatcc gcgtgggcac
      301 tctagcgagc cacgagatgg gagctttcac gcttcggcct cgcgcggaag acaccgtccc
      361 cgggaggccc cgccgcgccc gccgcggctc ccgctcccgc cccccccgct cgcttctggt
      421 acgcctcacg ttccagtgag acggtgactc gtcacgacgg ttgacgtggg cagcgggcgc
      481 tgatagcgtg ccggggtggc aaccgccagc ccggcactgg tcgatccttc acgctccacc
      541 ccggacgtgg ccgagcagcg ggcccgcgtc cgcgccatcg tcgcgccgcg cagtggcgag
      601 cgcggtctgg acatcggctg cggcttcggg ctgctctcct gcgagctggc ccgcgacgtg
      661 ggaccgaccg ggcgcgtcat gggcgtcgac tcggtcccgg ccatgatcgt ggcctgcgag
      721 gagcgcgcgc gccacgacgc ggtgaccggc cgcaccgagt tccgccaggc ggacgccgag
      781 gcgctgccgt tcgcgcccgg caccttcgac ttcgtcaccg ccatgcaggt ctacgagtac
      841 atgccggacg tcgagcgagg gctggccgag gccgcgcgcg tgctgaaggc ccggggccgg
      901 ctcgcggtgc tcgatacgga ctgggagtcc tgcgtgtggc actccggcga ccgggagcgg
      961 accgcccggg tgctccaggc gtgggagcag cgcttcgccc atccccacct gcccgcccgc
     1021 ctgcccgagc tgctgcgccg caccgggttc gcgctccgaa gcgtgacctt gatcccggtg
     1081 gtcaacgtcg aggcgagcga gcaaacgtat agcccgagca tgatcgacgt gatcgcgcgc
     1141 ttcgtggcca atcgcggcgg gatcaccgag gacgaggccg cgcgctgggc cgaggacgtc
     1201 cgcgcccagg cggtgcgcgg cgagtacttc ttcagcctct cccgctatct gttcctggcc
     1261 gagcgcacgc gaccgaaccg gccgtgacat tccgatgaga ttggaagtcg agctcgtgga
     1321 agaggacggc ggcctctacc gggccaccgc ggtgctctac cccgaggtga gcgtgaccgg
     1381 ccgcaccgag aaggaagcgc tgggcctgct cctcgacgcg gtcgccaagt acctcaagag
     1441 ccagcccgag aaaagtcagc ccgggcgcgc ctgagcgggc ctaccaggcg cgggcgactt
     1501 cagcgggcgg ggcgcgatcc cccctgcgtc ggcatcgcct ctgcgcccgg ccccgcggtc
     1561 acggcggcgg cgggcgggcg cgcctgcgtc ggcatgcgct cctccgcggg cgggtcggcg
     1621 ggccggggca cagcggccgg aacccgcacc ggacggggca cggtctccga cgccgcggcc
     1681 ggaattcgcg ccggcggcgg aacgggggtc tcgaccttcc gggtcgtcgg ggttaccgga
     1741 acgggtgcgg gcggagcggg cggaatcggg cgcgccaccg gaggcgcggg cgagatcgca
     1801 ggcgcgggca gcggggccac cggcgtgacg gcgatcggcg cggccggcgg gcgcggtggc
     1861 atgcggacgg tgccggggcg atacggatcg ggaaggctgg tctcgtagat gtagaagccg
     1921 gcgccggcca ccaccaggac caccgcggtc gccaccaggg ccgccgcggt ggagctgcgt
     1981 cgccggatgc gcgcgcgctg cgcggcctgg acccgctggc gatccagctc ccctcggcac
     2041 tcgacgcact cgaccaggtg cgtctcgaga agggcccact ccgtgagggg catctcgccc
     2101 gcgacgagga gcggaagagc ccggcgcacg tcctggcacc tcatcctgcc tccatcttcc
     2161 acatctcgcc cgcgctgtaa agccctgcgt tagcatgtgg cccatgctgc cagccggcga
     2221 gggcaacccg ttcctccacg caacggtcgg cgacatgctc gcgacccagg ccgcgcgcgt
     2281 gggagaccg
//
LOCUS       DATMCV010000798         5166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_509322, whole genome shotgun sequence.
ACCESSION   DATMCV010000798 DATMCV010000000
VERSION     DATMCV010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509322"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..3242
                     /locus_tag="VNU03_18285"
     CDS             63..3242
                     /locus_tag="VNU03_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:636..638,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP76474.1"
                     /translation="MDLSRRDFIKLSAGSLTGTAIGGIAGLGASLAPGLAQAQTLRIE
                     KAKATPSVCPYCSVGCATLVHTVGNKIVNIEGDPRSPHNEGTLCPKGAAIYQLHINPN
                     RPTKVLHRAPGAADWEVWDLDRAMDRVAQLVKKTRDETFIERLENGRLVNCTTAIFSL
                     GGATLDNEWNHIQQKLFRGLGIVAIENQARIUHSSSVPGLGTRLGRGAATMYPRSMAD
                     SDCVVIQGSNMAECHPVAFRWPMQAKAKGAKLIHVDPRFTRTSAMADIHAPIRAGSDV
                     AFLGGLIRHVLESERWNTEPFFREFVTHYTNAPTIIGDGFQDTEDLAGVFSGLMQYTP
                     GVKEWPLDGFVGQYDPKTWQYAGAPGGKPSPPAGPPFDPLVRSLLAPLPRRDETLQHP
                     RSVFQIVRRHFSRYTPEMVERVTGCPQATFLKVAETILANSGPDRTTSFAYAVAWTQH
                     TNGPQTIGCCALLQLLLGNVGRPGAGVMALRGHASIQGSTDMPTLYHSIHGYMNHPSA
                     LKAHGSLRDWLLTETPPRGYMANTPKFMVSYLKSMYGAAAAAGNDFGYDWHPKIVGDH
                     SHMAMFVAMNEGRVKGMLCVGQNPATSLNASVERKGLGKLEWLVVKDNWLTETATFWQ
                     SAPEVTSGQVRSQDIGTEVFFFPAAQVAEYEGSFTNTQRMLQWHYKAADPPGDCRTDL
                     WLTHQLALRLKKLYADSREPRDQGFRSLTWDFDSDDARERERGEPDAHKILKEINGYY
                     TGQTDRHIAGFGDLKDDGSTTCASWIYCGVFPDKDRNLAARKQPDPPGVIGAQLEWGW
                     AWPANRRILYNRASADREGRPWSERKKWVWWDGAKWTGRDVPDFALTKAPSAKADPTA
                     IGLDALSGGDPFIMKPDGVGWLYVPSGLLDGPLPTHYEPAESPVANPIYQQQSSPVLK
                     YWKLDGNALAAPGDARFPYIVTTYRLTEHYLSGAMSRWNPWLAELQPEFFIEISPELA
                     ALKGIGNTDRIRVWTPRGSVQGKALVTRRVRPFTIAGRVVHHLGMPFHWGYQGLVVGD
                     AANELTALVGDPNVSIHEAKAFVCNVEKA"
     gene            3242..4024
                     /locus_tag="VNU03_18290"
     CDS             3242..4024
                     /locus_tag="VNU03_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWP76475.1"
                     /translation="MAEPMGFYTDTTVCIGCKACEVACKEWNQLPARNGGVGVLSGDS
                     YDNTRALDGTHWRHVKFVEQFSPDRQDGRWLMMSDVCKHCVNAGCLEVCPTGAIMRTE
                     FDTVVIQADTCNGCRDCIGACPFGVIGINPVSGTAQKCTLCYDRMKVGLEPACAKACP
                     TKSINFGPIADLKQLARGRVGQLHEAGEGRAYLYGADETMLGGLNSFYLLVDRPEVYG
                     LPPDPRLPSRNLKRSALWSTLGAVALAVMGVFHFRERGRSHV"
     gene            4017..4901
                     /gene="nrfD"
                     /locus_tag="VNU03_18295"
     CDS             4017..4901
                     /gene="nrfD"
                     /locus_tag="VNU03_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWP76476.1"
                     /translation="MSSTFFTASPGWRWFIVAYFFLGGLAGGSYFLATLLDRFGTERD
                     RPLVRLGYLVAFPIVCLCGLVLTVDLGRPERFWHMLVQSQTWRPMLKTYSPMSSGAWV
                     ILLFSGCAFVSFAGVMAERGWPRWALLRHASPDARFGRLVSGAGAILGLYLAGYTGVL
                     LSVTNRPVWADTTLLGLTFLTSAASTSAALLILLGFWSGLARERLYPLERFDRSALIL
                     EVLAIVALVASLGRIATVWLDGWGMLLAAMVLGGIALPLLLDWRGPAGRPAWIAPTLV
                     LLGGLMLRMVIVFSSDRL"
     gene            4925..5143
                     /locus_tag="VNU03_18300"
     CDS             4925..5143
                     /locus_tag="VNU03_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76477.1"
                     /translation="MALLVVAGVAVACTSPESTRTRGGGPGADVGNHPRGPVQLHAGA
                     HMFAGTPTSGKTIGRHALIGGTEQAGQR"
BASE COUNT          788 a         1847 c         1758 g          773 t
ORIGIN      
        1 cggcgggaga tcaaaaccga cgacggctct gcacgagccg atcctcccga cgaggagcgc
       61 ccatggatct gtcacgacgg gacttcatca agctgtccgc gggcagcctc accggcaccg
      121 cgatcggcgg gatcgccggc ctcggggcga gcctggcccc cggcctcgct caggcgcaga
      181 cgctccggat cgagaaggcc aaggctaccc cgagcgtctg cccctactgc tcggtgggct
      241 gcgccaccct cgttcacacc gtgggcaaca agatcgtcaa catcgagggc gatccccgga
      301 gcccccacaa cgagggcacg ctctgtccga agggcgccgc gatctaccag ctccacatca
      361 acccgaaccg cccgaccaag gtcctgcacc gggcgccggg ggcggccgac tgggaggtat
      421 gggatctcga ccgggcgatg gaccgggtgg cgcagctggt caagaagacg cgcgacgaga
      481 ccttcatcga gcgcctcgag aatggccggc tggtcaactg cacgactgcc atcttctcgc
      541 tgggcggggc caccctcgac aacgagtgga accacatcca gcagaagctg ttccgcggcc
      601 tcgggatcgt ggccatcgag aatcaggccc gtatctgaca cagctccagc gtccccgggc
      661 tggggacacg gctgggacgc ggggcggcga cgatgtaccc gcgcagcatg gcggactcgg
      721 attgcgtggt gatccagggc tcgaacatgg ccgagtgcca cccggtggcg ttccgctggc
      781 cgatgcaggc gaaggccaag ggcgcgaagc tgatccacgt ggacccgcgc ttcacccgca
      841 ccagcgcgat ggccgacatc cacgcgccga tccgcgccgg ctccgacgtg gcgttcctcg
      901 gcggcctgat ccgccatgtg ctggagtccg agcggtggaa caccgagccg ttcttccggg
      961 aattcgtcac ccactacacc aacgcgccga cgatcatcgg cgacgggttc caggacaccg
     1021 aggacctcgc gggggtcttc tccggcctga tgcagtacac ccccggggtg aaggaatggc
     1081 ccctcgacgg gttcgtcggc cagtacgatc cgaagacgtg gcagtacgcg ggggcgccgg
     1141 gcggcaagcc ctcgccaccg gccgggccgc cgttcgaccc gctggtgcgc tcgctgctcg
     1201 ccccgctccc gcggcgcgac gagaccctgc agcacccccg gtcggtcttc cagatcgtcc
     1261 ggcggcactt cagccgctac acccccgaga tggtggagcg ggtgaccggc tgcccgcagg
     1321 cgaccttcct caaggtcgcc gagacgatcc tggccaactc cggccccgac cgcaccacct
     1381 cgttcgccta cgcggtggcg tggacccagc acaccaacgg accccagacc atcggctgct
     1441 gcgcgctgct gcagctactc ctgggcaacg ttggccgccc gggcgccggg gtgatggccc
     1501 tccgcggcca cgcctcgatc cagggctcga ccgacatgcc gacgctctat cactcgatcc
     1561 acggctacat gaaccatccc tccgcgctga aggcccacgg ctcgctccgc gactggctgc
     1621 tcaccgagac gccgccgcgg ggctacatgg ccaacacgcc gaagttcatg gtctcgtacc
     1681 tcaagtcgat gtacggagcg gcggccgccg ccgggaacga cttcggctac gactggcacc
     1741 cgaagatcgt cggggaccac tcgcacatgg cgatgttcgt ggccatgaac gagggccggg
     1801 tgaagggcat gctctgcgtc ggccagaacc cggccacctc gctcaacgcg agcgtggagc
     1861 gcaagggcct cggcaagctc gagtggctcg tggtcaagga caactggctc accgagaccg
     1921 cgaccttctg gcagagcgcg ccggaggtca cgagcggcca ggtgcggagc caggacatcg
     1981 gcaccgaggt cttcttcttc cccgccgccc aggtcgcgga gtacgagggg agcttcacca
     2041 acacccagcg catgctgcag tggcactaca aggcggccga tccgccgggc gactgccgga
     2101 ccgatctgtg gctgacccat cagctggccc tgcggctcaa gaagctgtac gcggactcca
     2161 gagagccgcg ggaccaggga ttccggagcc tgacctggga cttcgactcg gacgacgcgc
     2221 gcgagcgcga gcgtggcgag cccgacgccc acaagatcct caaggagatc aacgggtact
     2281 acaccggcca gaccgaccgc cacatcgccg gcttcggcga cctcaaggac gacggttcca
     2341 ccacctgcgc ctcctggatc tactgcgggg tgttccccga caaggaccgc aatctcgccg
     2401 cccgcaagca gcccgatccc cccggcgtca tcggcgcgca gctcgagtgg ggctgggcgt
     2461 ggccggccaa ccgccggatc ctctacaatc gcgcctccgc ggaccgggag ggccggccgt
     2521 ggagcgagcg caagaagtgg gtgtggtggg acggggccaa gtggacgggc cgggacgtcc
     2581 cggatttcgc cctgaccaag gccccgagcg cgaaggccga tccgactgcg atcgggctcg
     2641 acgcgctgtc cggcggcgat cccttcatca tgaagcccga cggggtcggc tggctgtacg
     2701 tgccgagcgg cctcctggat ggtcccctcc ccacccacta cgagccggcc gagtcgccgg
     2761 tcgccaaccc gatctaccag cagcagtcga gcccggtgct caagtactgg aagctcgacg
     2821 gcaacgccct ggccgccccc ggcgacgcgc gcttccccta catcgtgacc acgtaccggc
     2881 tcaccgagca ctacctgtcc ggggccatga gccgctggaa cccgtggctc gcggagctgc
     2941 agccggagtt cttcatcgag atcagcccgg agctggccgc gctgaagggc atcggcaaca
     3001 cggaccggat ccgggtctgg accccgcgcg gcagcgtgca gggcaaggcc ctcgtgaccc
     3061 ggcgggtgcg cccgttcacc atcgcgggcc gcgtcgtgca ccacctcggc atgccgtttc
     3121 actgggggta tcaggggctg gtggtgggcg acgcggccaa cgagctgacc gccctggtgg
     3181 gcgaccccaa cgtgtcgatc cacgaggcga aggccttcgt gtgcaacgtg gagaaggcgt
     3241 gatggcggag ccgatgggct tctacaccga caccacggtc tgcatcggat gcaaggcgtg
     3301 cgaggtggcg tgcaaggagt ggaaccagct accggcccgg aacggcggcg tcggcgtcct
     3361 cagcggggac agctacgaca acacccgcgc gctcgacggc acccactggc gccacgtgaa
     3421 gttcgtggag cagttcagcc ccgatcgcca ggacggccgc tggctgatga tgagcgacgt
     3481 ctgcaagcac tgcgtgaacg ccggctgcct cgaggtgtgc cccaccggcg cgatcatgcg
     3541 gaccgagttc gacaccgtgg tcatccaggc ggacacctgc aacggctgcc gggactgcat
     3601 cggcgcgtgc ccgttcggcg tgatcgggat caacccggtg tcgggcaccg cgcagaagtg
     3661 cacgctctgc tacgaccgga tgaaggtggg cctcgagccg gcgtgcgcca aggcgtgccc
     3721 gaccaagtcc atcaacttcg gcccgatcgc ggacctgaag cagctggccc gcggccgggt
     3781 gggccagctc cacgaggccg gcgaggggcg cgcgtatctc tacggggccg acgagaccat
     3841 gctcggcggg ctcaactcct tctacctgct ggtggacagg ccggaggtct acgggctccc
     3901 gccggatccg cgcctgccgt cgcgcaacct caagcggtca gcgctctggt ccacgctggg
     3961 ggcggtggcg ctcgcggtca tgggcgtctt ccacttccgg gagcgcggga ggagtcatgt
     4021 ctagcacgtt cttcaccgcg tcgcccggat ggcgctggtt catcgtcgcc tacttcttcc
     4081 tgggcgggct cgcgggcggg agctatttcc tggccaccct gctcgatcgc ttcgggaccg
     4141 agcgagaccg gcccctcgtg cggctcggct atctcgtcgc gtttccgatc gtgtgcctgt
     4201 gcgggctcgt cctcaccgtc gatctcgggc ggccggagcg cttctggcac atgctggtgc
     4261 agtcccagac ctggcggccg atgctcaaga cctactcccc gatgtcgtcc ggcgcgtggg
     4321 tgatcctgct cttcagcgga tgcgccttcg tgtcgttcgc gggggtgatg gccgagcgtg
     4381 gctggccccg gtgggcgctt ctgcgccacg ccagcccgga cgcccggttc gggcggctcg
     4441 tgagcggcgc gggcgccatc ctcggtctgt atctggcggg ctataccggc gtgctgctct
     4501 cggtcaccaa ccgacccgtc tgggcggata ccacgctgct cgggttgacc ttcctgacct
     4561 cggccgcgtc cacctcggcc gcgctcctca tcctgctcgg attctggagc gggctcgcgc
     4621 gcgagcgcct gtatccgctc gagcgcttcg atcgctcggc gctgatcctc gaggtcctcg
     4681 cgatcgtcgc gctggtcgcc tcgctcgggc gcatcgccac cgtgtggctc gacgggtggg
     4741 gcatgctcct ggccgcgatg gtcctgggcg gcatcgcgct gcccctgctc ctcgactggc
     4801 gagggccggc cggtcggccg gcctggatcg ctcccacgct cgtcctgctc ggcgggctca
     4861 tgctacgcat ggtcatcgtc ttctcctcgg atcggctatg agccggcgga ggtggcgcgg
     4921 ggtcgtggca ctgctcgtcg tcgcgggggt ggccgtcgcc tgcaccagcc ccgagtcgac
     4981 ccggactcgg ggcggcggac ccggcgccga cgtcggcaat catccgcgcg ggccggtcca
     5041 gcttcatgcc ggcgcccaca tgttcgccgg cacgcccacc tccggcaaga cgatcggccg
     5101 acacgccctc atcgggggca ccgagcaggc cgggcagcga taggcgcatg atcgcgaccc
     5161 ggtcgg
//
LOCUS       DATMCV010000799         2236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_446783, whole genome shotgun sequence.
ACCESSION   DATMCV010000799 DATMCV010000000
VERSION     DATMCV010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446783"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..454
                     /locus_tag="VNU03_18305"
     CDS             332..454
                     /locus_tag="VNU03_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76478.1"
                     /translation="MPDLLAVRSQMVMSLGFHLLFAMVGVAMPSWPGGSCFSPR"
     gene            495..1343
                     /locus_tag="VNU03_18310"
     CDS             495..1343
                     /locus_tag="VNU03_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWP76479.1"
                     /translation="MSLTVFSVAVLFTSLGAGVVGAILGLGGGILLVPILTIFYGVDL
                     HYAMGASIISVIATSSGAAASYLRTGLSNLRVGLFLVVATITGALTGAFLAGVVPTRW
                     LELILGLALSYSAFTTIRQLGDELPRDLSADGLAIRFGLEGTYHDLRLDREIPYRARN
                     VAGGFVAMYGAGVLSGMLGIGSGAFKVLAMDYLMRLPMKVSTATSNFMIGLTAAASAG
                     IYFSRGDIHVLIVAPVAVGVLAGAYLGTTVMARMRNTTVRKLFLPIVVYLALSMILRG
                     LGIHLL"
     gene            1358..1747
                     /locus_tag="VNU03_18315"
     CDS             1358..1747
                     /locus_tag="VNU03_18315"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HWP76480.1"
                     /translation="MFPVEYLVARVLLVGGLLGLGLIVLGLGLYAAHGGFHQHVLLLS
                     RPAGDAPPGVFTSLRQIGVGLQHRPIDPLALSALGLVLLMATPGAAVTLAIPGFLAVR
                     DFRYATIAALVLAMLLVSLLLAGAIHS"
     gene            complement(1757..>2236)
                     /locus_tag="VNU03_18320"
     CDS             complement(1757..>2236)
                     /locus_tag="VNU03_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76481.1"
                     /translation="DGLRGVPAERSRARLIRWRERQESARDRLRYYRGRLGELARADL
                     EERAALVGRMIGRQLAARNGAAAAGRGERAVPLVPDLTARYRRAVQAYMPAPYPGRLV
                     VLRSQYLDDPRPDLGWSQLSPRVEVHAVPGDHLGCITRHMAETAGRLGACLEAVALG"
BASE COUNT          268 a          823 c          774 g          371 t
ORIGIN      
        1 ttccggggtc ggcattcgta gatctgcacc ccccttgccg cgacgatcat cgcgagcgat
       61 tcgcccgcgc cgggattgag cttctccggc accgcgaccg cggacgtcgg gcgttgcggg
      121 ctcgcgcacg ccccgacgag aaccaccagc atgacgccca tcacgccccg tgcaatcatc
      181 atcgcgccct ccgtcgcgtg gacagagagt ggcgtgctcg tcggggggtg tcaagcgtga
      241 gcgtgtcctt acagcaccgc gcttccgcgg tgatgagcaa gaacccgcga atgcggcccg
      301 cgcggcggcg ggctaccatg gccacagagc catgcccgat ctcctcgccg tgcgctcgca
      361 gatggtcatg tcgctggggt tccacctcct cttcgcgatg gtgggcgtgg ccatgccctc
      421 ctggccgggg ggctcgtgct tttcccctcg ctgatctatc ttcttcgggt gttcaagcgc
      481 gagccgaccc ggtcgtgagc ctcaccgtct tctcggtggc ggtgctcttc acgtcgctgg
      541 gggcgggtgt ggtgggggcc atcctggggc tgggcggcgg catcctgctg gtgccgatcc
      601 tcaccatctt ctacggggtg gatctgcact acgcgatggg ggccagcatc atctcggtca
      661 tcgccacgtc gagtggagcg gcggcgtcct acctgcgcac cggcctcagc aacctccgcg
      721 tcggcctgtt cctggtggtg gccaccatca ccggcgcgct caccggcgcc ttcctggcgg
      781 gagtcgtgcc cacccgctgg ctcgagctga tcctggggct cgcgctctcc tactccgcgt
      841 tcaccaccat ccggcagctc ggggacgagc tgccgcggga cctctcggcg gacgggctcg
      901 cgatccggtt cggcctcgaa ggcacctacc acgacctgcg cctcgaccgc gagatcccct
      961 accgcgcgcg caacgtggcg ggcggcttcg tggccatgta cggggcgggt gtgctatccg
     1021 gcatgctcgg catcggcagc ggcgcgttca aggtgctcgc gatggactac ctgatgcgcc
     1081 tgcccatgaa ggtctccacc gcgaccagca acttcatgat cgggctgacc gcggcggcca
     1141 gcgcgggcat ctacttcagc cggggcgaca tccacgtgct gatcgtggcc ccggtcgcgg
     1201 tgggcgtgct ggccggcgcc tacctgggca ccaccgtgat ggcgcgcatg cgcaacacca
     1261 cggtgcggaa gctcttcctg cccatcgtgg tgtacctggc gctctcgatg atcctgcgcg
     1321 gtctcggcat ccacctgctg tgaccacgcc ccggtccgtg ttccctgtcg agtacctcgt
     1381 cgcgcgcgtg ctgctcgtgg gggggctcct cgggctcggc ctgatcgtgc tcgggctcgg
     1441 gctctacgcc gcacatgggg gctttcacca gcacgtgctg ctgctgagcc gcccggccgg
     1501 cgacgcacca cccggggtgt tcacctcgct gcgacagatc ggggtcgggc tgcagcaccg
     1561 gcccatcgat ccgctcgcgc tgagcgccct cggcctggtg ctgctgatgg ccaccccggg
     1621 cgccgcggtc accctggcga ttccgggctt cctggccgtg cgggacttcc gctacgcgac
     1681 gatcgccgcg ctggtgctgg cgatgctgct ggtgagcctg ctcctggccg gcgccattca
     1741 cagctagcgc cgggtcctaa ccgagcgcga ccgcctcgag gcaggcgccg agccgcccgg
     1801 cggtctccgc catgtggcgc gtgatgcagc ccaggtgatc gccgggcacc gcgtgcacct
     1861 ccacgcgcgg gctgagctgc gaccagccga gatccgggcg cgggtcgtcc aggtactgcg
     1921 agcgcagcac cacgaggcgc ccgggatagg gtgcgggcat gtacgcctgc acggcgcgcc
     1981 ggtagcgggc ggtgagatcg gggacgagcg gcaccgcacg ctccccacgc ccggcggccg
     2041 ccgcgccgtt ccgggcggcc agctgccggc cgatcatccg accgaccagc gccgcgcgct
     2101 cctcgagatc cgcgcgggcc agctcgccga gccggccgcg gtagtagcgg agccggtcgc
     2161 gcgccgattc ctggcgctcg cgccagcgga tcagccgcgc gcggctccgc tccgcgggca
     2221 cgccgcgaag gccatc
//
LOCUS       DATMCV010000800         3751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_41442, whole genome shotgun sequence.
ACCESSION   DATMCV010000800 DATMCV010000000
VERSION     DATMCV010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41442"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..963
                     /locus_tag="VNU03_18325"
     CDS             199..963
                     /locus_tag="VNU03_18325"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76482.1"
                     /translation="MIIARDITRRFTHGAAGAFVALDRVSFEVAQGEFLAIVGPSGCG
                     KSTLLNIVAGLKRPDGGTVTVNGRDTTGTTPSEMGYLFQEDTVLPWFSVERNIGLGLE
                     YRGISRADARPRVEWALEAAGLTEFRASYPHQLSGGMRRRVALMMTLVVDPKILMMDE
                     PFGALDTHTKTRLHASLLEIWEKTRQTIIFVTHDLMEAITLSDRVIIMSGRPGRVKAS
                     YPIRIPRPRDVIKVKDSEEYLGDFREIWRILGEEFV"
     gene            1034..2074
                     /locus_tag="VNU03_18330"
     CDS             1034..2074
                     /locus_tag="VNU03_18330"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HWP76483.1"
                     /translation="MRRPYRQPGLVLRPRSGPPDPLAKILQEDLRSLGYLRAGIDGRF
                     GDGTASAIRALQQDLLTAGQHGSDGDAPVALQTFNRGRVTGLTGVVDERLAACIEDIL
                     DDHRIPALPRSTEPAQANQAAMAQIGQLVGLPVPRPFLLATFLQESGGLHFRVPSGDN
                     ADNFIVVGLDHGDHGRPDRITSRGYGIGQFTLFHHPPTATEVATVMVDPVKNVQRAVK
                     ELRSKYELFVAGSTSGQQADDRIHEIGDGALRPCRFAPDDPRYMTDCARCAAEHLVDI
                     PAGAPVYRGAAITMHPTQYHPETAYSRVPDRAQLGCDWPYAVRRYNGSGVNSYHYQFQ
                     VLQRLTRPPVSA"
     gene            2071..3267
                     /locus_tag="VNU03_18335"
     CDS             2071..3267
                     /locus_tag="VNU03_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HWP76484.1"
                     /translation="MKTIVFCADGTWNGPGQPEEQEEQGGRRTNVFKLFLNLAGDLSP
                     DSLRSADEQERIVAGADGKPTQIAKYLHGVGDSKNFLVRVLGGGGGAGLVTRIVRGYT
                     FISRNFEPGDRIFIVGFSRGAYTARALAGLIAARGLLDPTQNDLAGDRAQAYRLGSAE
                     WFTYRREVLKERKSLLRTMAEVAVDLPGFLQSPAVAPRISDVPLEGVVVWDTVGSLGI
                     PAYNLKTDTQLDAFQFADLALSRKVRHGLHAVSVDEQRSNFTPTLWDHDDRIVQVLFP
                     GAHADVGGGYPDSESGLSDGGLQWVVEELTKLGVAFAAEPAVTLKPDACGTAHAPWAQ
                     APWTLLPNGHRAFPPGLAVHRSVVDRLAGADRLRAAGQPVYNPESLADAYVAAGQLKP
                     GLRVIG"
     gene            complement(3257..>3751)
                     /locus_tag="VNU03_18340"
     CDS             complement(3257..>3751)
                     /locus_tag="VNU03_18340"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWP76485.1"
                     /translation="LLSFVYTRCRDAWGCPLAYRVFDAVGAAVERSPDLRSRVRLVTL
                     SFDPRHDTPAVMRQYAGEQAARGLDWRFLTTRSERALAPLLGGFGQDVRAAAAAPAHD
                     AGELTHVLKVFLIDPRGVVREIYSTAYLYPEVVVADIETLRLEGRSGNAAPATRAAGA
                     PISR"
BASE COUNT          545 a         1344 c         1327 g          535 t
ORIGIN      
        1 tgggcttcat catcacggtc gccctcggcc agctcgaggc caccgatctc ttcgtcgcgc
       61 tgctcaccct gtgcgtgctc gcggtggtgc tgatccatct ggcctcgcgg ctcgagcggc
      121 ggctcctgca ctggcgcccc gagcacgtcg ggcacgaggg cggggtctag cccggtggcg
      181 gcgccggtcc ccgctcccgt gataatcgcg cgcgacatca cccgccgctt cacccacggc
      241 gcggcgggcg ccttcgtcgc gctcgaccgg gtctcgttcg aggtcgccca gggcgagttc
      301 ctggccatcg tcggcccgtc ggggtgcggc aagtccaccc tgctcaacat cgtggccggc
      361 ctcaagcgcc cggacggcgg cacggtgacc gtcaacggcc gggacaccac cggcacgact
      421 cccagcgaga tgggctatct cttccaggag gacacggtgc tgccgtggtt ctcggtcgag
      481 cgaaacatcg ggctcggcct cgagtaccgc gggatctccc gcgccgacgc caggccgcgc
      541 gtggaatggg cgctcgaggc ggcggggctc accgagttcc gcgcctcgta cccgcaccag
      601 ctctcgggtg gcatgcgccg gcgggtggcc ctcatgatga cgctggtggt ggatcccaag
      661 atcctcatga tggacgagcc cttcggcgcg ctcgacaccc acaccaagac gcggctgcac
      721 gcctcgctgc tcgagatctg ggagaagacg cggcagacca tcatcttcgt gacccacgac
      781 ctgatggagg cgatcacgct gtcggaccgg gtcatcatca tgagtggccg accggggcgg
      841 gtgaaggcct cctacccgat ccgcatcccg cgcccccgcg acgtcatcaa ggtgaaggac
      901 agcgaggagt acctgggcga tttccgggag atctggcgga tcctcggcga ggagttcgtc
      961 tagctactgc agcatctcga tcaactccga atgttgacag ggccccgggg cggttcgtag
     1021 gctgggaaga ccgatgaggc gtccgtatcg tcagcccggg ctcgtgctgc ggccccgatc
     1081 gggacccccc gatcccctcg ccaagatcct gcaggaggat ctgcggtcgc tcggctacct
     1141 gcgggccggc atcgacggcc ggttcggcga tggcaccgcg agcgcgattc gcgcgctgca
     1201 gcaggatctc ctgaccgccg ggcagcacgg aagcgacggc gacgccccgg tggcgctgca
     1261 gaccttcaat cgcggccgcg tgaccggcct gaccggggtg gtcgacgagc ggctggccgc
     1321 ctgcatcgag gacatcctgg acgaccaccg gatcccggcg ctgccccgct ccaccgagcc
     1381 cgcgcaggcc aaccaggccg cgatggccca gatcggccag ctcgtggggc tgcccgtccc
     1441 gcggccgttc ctgctcgcga cctttctgca ggagagcggc ggcctccact tccgggtgcc
     1501 gagcggcgac aacgcggaca acttcatcgt ggtcgggctc gatcacggcg atcacggccg
     1561 tcccgaccgc atcacgtcgc gcggctacgg gatcgggcag ttcacgctct ttcatcaccc
     1621 gccgaccgcg accgaggtcg ccaccgtcat ggtggacccg gtcaagaacg tccagcgcgc
     1681 ggtgaaggag ctgcggagca agtacgagct cttcgtcgcg ggctccacct ccggccagca
     1741 ggccgatgac cggattcacg agatcggcga cggtgccctc cggccctgcc gcttcgcgcc
     1801 ggacgatccg cgctacatga ccgactgcgc gcggtgtgcc gccgagcacc tcgtcgacat
     1861 cccggcgggg gctccggtct accgcggggc cgcgatcacg atgcatccga cccagtacca
     1921 cccggagacg gcatactccc gggtgccgga ccgggcgcag ctcggctgcg actggcccta
     1981 cgcggtgcgg cgctacaacg ggtccggcgt caactcgtac cactaccagt tccaggtcct
     2041 gcagcggctg accaggccgc cggtgagcgc atgaagacca tcgtgttctg tgcggacggg
     2101 acgtggaatg gacccgggca gcccgaggag caggaggagc agggcggccg tcggaccaac
     2161 gtcttcaagc tcttcctgaa tctggccggc gacctctcgc cggacagcct gcgctcggcc
     2221 gacgagcagg agcggatcgt ggcgggggcc gacgggaagc ccacgcagat cgccaagtac
     2281 ctgcacgggg tcggcgactc gaagaacttc ctggtacggg tgctcggcgg tgggggcggc
     2341 gcggggctcg tcacgcggat cgtgcgcggc tacacgttca tctcccgcaa cttcgagccg
     2401 ggggatcgca tcttcatcgt cgggttcagc cggggcgcgt acaccgcgcg tgccctcgcg
     2461 ggcctgatcg cggcgcgagg gctcctggac cccacgcaaa acgatctcgc cggcgaccgg
     2521 gcgcaggcct atcgcctggg ctccgccgag tggttcacgt accggcgcga ggtgctgaag
     2581 gagcgcaaga gcctgctgcg caccatggcc gaggtcgcgg tcgatctgcc cggcttcctg
     2641 cagtcgcccg ccgtcgcgcc gcggatcagc gacgtgccgc tcgagggggt cgtggtgtgg
     2701 gacacggtcg gatcactcgg gattcccgcc tacaacctga agaccgacac gcagctcgac
     2761 gcgttccagt tcgccgacct cgcgctgagc cggaaggtgc gccacggcct ccacgcggtc
     2821 tcggtcgacg agcagcggtc gaacttcacc ccgaccctct gggaccacga cgatcgcatc
     2881 gtccaggtgc tgttcccggg cgcgcacgcg gacgtgggcg gcggctaccc ggatagcgag
     2941 agcggcctgt ccgacggcgg cctgcagtgg gtggtcgagg agctgaccaa gctgggcgtc
     3001 gcgttcgccg cggagccggc ggtcacgctc aagcccgacg cgtgcgggac cgcgcatgcg
     3061 ccgtgggccc aggccccgtg gactctgctg cccaacggcc atcgcgcgtt tccaccgggg
     3121 ctggccgtcc accgctcggt ggtggaccgg ctcgccggtg cggatcggct gcgggccgcc
     3181 gggcagccgg tctacaaccc cgagagcctc gccgacgcct acgtcgcggc gggccagctc
     3241 aagccgggcc tgcgcgtcat cggctgatcg gcgcgccggc ggcgcgggtg gccggcgccg
     3301 cgttcccgga tcggccctcc aggcggagcg tctcgatgtc ggcgaccacc acctccgggt
     3361 agaggtaggc ggtgctgtag atctcgcgca ccacgccgcg cgggtcgatc aggaagacct
     3421 tcagcacgtg ggtcagctcg cccgcgtcgt gcgccggcgc ggccgcggcg gcgcgcacgt
     3481 cctgcccgaa gccgccgagc aagggagcca gcgcgcgctc cgagcgcgtg gtgaggaatc
     3541 gccagtcgag cccccgcgcg gcctgctcgc ccgcgtactg ccgcatcacc gccggggtgt
     3601 cgtgccgggg atcgaaggag agcgtgacca gccgcacgcg cgagcggagg tccggggagc
     3661 gctcgaccgc ggcgcccacc gcgtcgaaga cccgataggc gaggggacag ccccacgcgt
     3721 cgcggcagcg cgtgtagacg aagctcagca g
//
LOCUS       DATMCV010000801         5314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_509572, whole genome shotgun sequence.
ACCESSION   DATMCV010000801 DATMCV010000000
VERSION     DATMCV010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509572"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1255
                     /locus_tag="VNU03_18345"
     CDS             131..1255
                     /locus_tag="VNU03_18345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76486.1"
                     /translation="MIADGGLKIHGASLEARGLSKAFGGARVVSDVSFRVEGGALLTL
                     LGPSGSGKTTTLRMIAGFEDPDAGQILVADEDITERPAHRRNIGVVFQQYALFPHLTV
                     AQNVAYPLEMRRYGRAEIRSRVGAALDLVRLQGYETRYPRQLSGGQQQRVALARAIVF
                     EPPVLLMDEPLGALDKRLRETMQVEVRRLQQQLGITTVSVTHDQVEALVMSDLIAVMD
                     GGVLHQLASPLEVYQRPATQFVADFIGESNLLVGALSEPAGGALTFTTAKGLRIQTQG
                     GAGATRLPGATQLAGATHLIIRPEHVRIGPDAERAANRYAAEVLEALYVGDLVKYRVV
                     AETGDELWAKTLAPATGQRWSKGQRVTVGWEPGDSLTVSA"
     gene            1277..2347
                     /locus_tag="VNU03_18350"
     CDS             1277..2347
                     /locus_tag="VNU03_18350"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76487.1"
                     /translation="MGKTEISRRRFLKMTGLTAAAAGAASVAFPHVITTAKGAANELV
                     FVGFGGGYQEGQTKALFEPFEKETGIKVVQTTGVDLAKLRAQVQSKNVEWDLISIPDR
                     LRYTAVQDGLLQKLNYGMINAKDIQKDLVTEYAVGCVTIPMLLTYSTKAHPPGKEPKT
                     WMDYWDPAKVPGIRGMYNAPPYSLEFALIADGVPKDKLYPLDVTRAFKSFDRIKPAVK
                     VWWSQFPQPGVLLQSGEITMTPWTRSITPVFEGQPMGVSYDGAALTYEGWVVPTGAPN
                     AQNAMKFINWALQPKPQAELTKFVAFGPTNKNATQFVNPKLRPLLSSDPENVKKGFLL
                     SGDWWGPNLEKVTEAWNEWRLK"
     gene            2358..3137
                     /locus_tag="VNU03_18355"
     CDS             2358..3137
                     /locus_tag="VNU03_18355"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP76488.1"
                     /translation="MSTVLYEPKGAVVRITLNRPEAMNAFDLPTARELGRRLEEFDRD
                     DALRVAIVTGAGDKAFCAGADLKQMHGGSHAGGIDELWGSEIQYRLGQRLQVTKPVIA
                     AINGYCLAGGLELALGCDIRIASETASFGCPEVRWSILHGFGAMRLPSTVPMSVAMEM
                     LLTGERIDAARAREIGLVSRVVAAKALLPTAEQMAERISQNGPLAIKLTKELAWRSLH
                     QHPDDALRFYGAVTALMHETEDSKEGPRAFAEKRTPRFKGK"
     gene            3134..5257
                     /locus_tag="VNU03_18360"
     CDS             3134..5257
                     /locus_tag="VNU03_18360"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWP76489.1"
                     /translation="MTTRHTLDSFTNPASVAVIGASTVLHKAGGRRWRSMVEAGFTGP
                     LYPIHPTATEILGHKAYRSLTDVPAPPELAVVLVRPDLVPGALADCGRLHVPAVVVIT
                     AGFGETGPEGKAVEQAMARGLRAAGGRMIGPNCAGLFSASGRVNVLGWGVPAGPIAVI
                     SQSGNMALTFVQLAREKGIGMSKLITVGNAADLRIPEYLEYLQDDPDTRVIVAYLEGF
                     EPGEGRAVWELMRRRPHPKPVVVIKPGETDSGRRAALSHTGALAGQHRVVAAALRQCG
                     ILRVTDSEEAWDAAMALALLPAPDPGAVVVISDGGGHATIVADTADKVGLAVPPLSAA
                     TREALAAVLPQRSAIGNPVDFAGVAEEEPEVVPRVVDIALGDPAIGGALIAGHFGGYV
                     KIATEELGRRECAAALELTRVVARRGKPVIVHTIYGAEPLPALEPLRAAGVPIYRSLE
                     ASARAMGALWQHARAQRARAGAGPRRSQPDAARVSALLAGARPGEVLPEPECRELVAC
                     YGIPVPPWQVVTGAEETAAAVEALAAPAALKLVARGVVHKSDVGGVLLGIRGAAAARD
                     GWARLMDAAARAGATDARVLVTPMLDPGVEAVVGVLRDRQFGPVVMFGLGGVLVEALD
                     DVVFRVAPFGAGEAEAMISEIHGHRLFGPVRRRPPVDRTALVDLLVRVSELASDRPEL
                     AELDLNPVICSARGAALADARAVVG"
BASE COUNT          768 a         1889 c         1948 g          709 t
ORIGIN      
        1 aaggcgcgaa ccggcttcga gctgctggtg cccgaccggg tgccgatcac cggctccccc
       61 gacgccgacg agctcgacat cctgcgccgg cgggtcgacc cgcagggcct gctgcgccgc
      121 tagggcggga atgatcgccg acggcgggct caagatccac ggtgcctccc tcgaagcgcg
      181 ggggctctcc aaggcgttcg gcggtgcgcg cgtggtcagc gacgtgtcgt tccgcgtcga
      241 gggtggggcg ctgctcaccc tgctcgggcc cagcggctcc gggaagacca ccaccctgcg
      301 catgatcgcg ggcttcgagg atccggacgc cgggcagatc ctggtcgcgg acgaggacat
      361 caccgagcgg cccgcccacc gccggaacat cggcgtggtg ttccagcagt acgccctctt
      421 cccccacctg accgtcgcgc agaacgtggc ctacccgctc gagatgcgcc gctacggccg
      481 ggccgagatc cggagccggg tgggcgcggc gctcgacctc gtccgcctgc agggctacga
      541 gacgcgctac ccgcgtcagc tctccggcgg ccagcagcag cgggtagccc tggcccgcgc
      601 catcgtcttc gagccgccgg tgctcctgat ggacgagccg ctcggcgcgc tcgacaagcg
      661 gctccgcgag accatgcagg tcgaggtgcg ccggctccag cagcagctcg gcatcaccac
      721 cgtgtcggtc acccacgacc aggtggaggc cctggtgatg tccgacctca tcgcggtgat
      781 ggacggcggc gtgctgcatc agctcgcctc gccgctcgag gtgtaccagc gcccggcgac
      841 ccagttcgtg gccgacttca tcggcgaatc gaacttgctg gtgggcgcgc tctccgagcc
      901 ggcgggcggt gcgctcacct tcaccaccgc caagggcctc cggatccaga cccagggcgg
      961 cgcgggagcc acgcgtctgc ccggagccac gcaactggcc ggagcgacgc atctgatcat
     1021 ccggccggag cacgtgcgga tcgggccgga cgcggagcgg gcggccaacc gctacgcggc
     1081 agaggtgctc gaggccctgt acgtgggaga cctcgtgaag taccgggtgg tcgcggagac
     1141 cggcgacgag ctctgggcga agaccctggc tcccgcgacc gggcagcgat ggagcaaggg
     1201 gcagcgggtg acggtcggct gggagccggg cgacagcctc accgtgagtg catagacccg
     1261 atcgaaagga gtcgcgatgg gcaagacgga gatctcgcgt cgcaggttct tgaagatgac
     1321 gggcctgacc gcggccgcgg cgggcgcggc cagcgtggcg ttcccccacg tcatcaccac
     1381 cgccaagggg gccgcgaacg agctggtgtt cgtgggcttc ggcggcggct accaggaggg
     1441 ccagaccaag gcgctcttcg agccgttcga gaaggagacc ggcatcaagg tggtgcagac
     1501 caccggagtc gacctggcca agctgcgggc gcaggtgcag tcgaagaacg tggagtggga
     1561 tctcatctcc atcccggatc ggctgcgcta caccgcggtc caggacgggc tgctccagaa
     1621 gctcaactac gggatgatca acgccaagga catccagaag gacctcgtca ccgagtacgc
     1681 ggtcggctgc gtgaccatcc ccatgctgct cacctacagc accaaggccc atccgcccgg
     1741 caaggagccg aagacctgga tggactactg ggacccggcc aaggtgccgg ggatccgcgg
     1801 catgtacaac gccccgccct actcgctcga gttcgcgctc atcgcggacg gcgtgccgaa
     1861 ggacaagctc tacccgctcg acgtgacccg cgccttcaag agcttcgacc gcatcaagcc
     1921 cgcggtgaag gtgtggtgga gccagttccc gcagccgggg gtgctgctcc agagcggcga
     1981 gatcaccatg accccgtgga cgcgctccat caccccggtc ttcgagggcc agccgatggg
     2041 cgtcagctac gacggggcgg ccctgaccta tgaaggatgg gtggtgccga ccggcgcgcc
     2101 caacgcgcag aacgcgatga agttcatcaa ctgggcgctc cagccgaagc cgcaggctga
     2161 gctgaccaag ttcgtggcgt tcggccccac caacaagaac gcgacccagt tcgtgaaccc
     2221 caagctccgc ccgctgctct cctcggatcc cgagaacgtg aagaaggggt tcctcctgag
     2281 cggggactgg tggggcccga acctcgagaa ggtcaccgag gcgtggaacg agtggcgcct
     2341 caaataggag acgacctgtg agcaccgttc tctacgagcc caagggcgcc gtggtccgga
     2401 tcacgctgaa ccggcccgag gccatgaacg cattcgacct gccgacggcc cgcgagctgg
     2461 gccgtcggct cgaggagttc gaccgcgacg acgcgctccg cgtcgcgatc gtcaccggcg
     2521 cgggcgacaa ggcgttctgc gccggcgccg atctcaagca gatgcacggc ggctcgcacg
     2581 ccgggggcat cgacgagctg tgggggtcgg agatccagta ccggctcggc cagcggctcc
     2641 aggtcacgaa gccggtgatc gccgcgatca acggctactg cctcgccggg gggctcgagc
     2701 tggccctcgg ctgcgacatc cgcatcgcct ccgagaccgc gtcgttcggc tgcccggagg
     2761 tgcgctggtc gatcctccac ggcttcgggg cgatgcggct gccgtccacc gtgccgatgt
     2821 cggtggccat ggagatgctc ctcaccggcg agcgcatcga cgcggcgcgc gcgcgggaga
     2881 tcgggctggt gagccgggtg gtggccgcga aggcgctgct gcccaccgcg gagcagatgg
     2941 cggagcgcat cagccagaac ggcccgctcg ccatcaagct caccaaggag ctggcgtggc
     3001 ggtcgctgca ccagcacccg gacgacgcgc tccgcttcta cggcgcggtc accgcgctga
     3061 tgcacgagac ggaggactcc aaggaaggtc cgcgggcctt cgcggagaag cgcacgcccc
     3121 gtttcaaggg caaatgacga cccggcacac cctcgactcc ttcaccaacc cggccagcgt
     3181 ggccgtcatc ggcgcctcca ccgtgctcca caaggcggga gggcgccggt ggcggagcat
     3241 ggtcgaggcg ggcttcacgg gcccgctcta cccgatccat cccaccgcga ccgagatcct
     3301 gggccacaaa gcctaccggt cgctcaccga cgtgccggcg ccccccgagc tcgcggtggt
     3361 gctggttcgc cccgacctcg ttcccggcgc cctcgcggac tgcggtcgcc tgcacgtgcc
     3421 cgcggtggtg gtcatcaccg cgggcttcgg ggagaccggc cccgagggca aggcggtgga
     3481 gcaggcgatg gcccgggggc tgcgcgcggc gggcgggcgc atgatcggcc ccaactgcgc
     3541 cggcctcttc agcgcctcgg gccgcgtgaa cgtgctcggg tggggcgtgc cggcgggccc
     3601 catcgcggtg atctcgcaga gcggcaacat ggcgctgacc ttcgtgcagc tcgcccgcga
     3661 aaaaggcatc gggatgagca agctcatcac ggtcggcaac gcggccgatc tgcgcatccc
     3721 cgagtacctc gagtatctcc aggatgatcc ggacacccgg gtgatcgtgg cgtacctcga
     3781 aggcttcgag cccggcgagg gccgcgcggt ctgggagctg atgcggcgcc ggcctcatcc
     3841 caagccggtg gtggtcatca agccgggcga gaccgacagc ggtcggcgcg cggcgctctc
     3901 gcacaccggc gcgctcgccg gccagcaccg cgtggtggcg gccgccctgc gccagtgcgg
     3961 catcctgcgc gtcaccgaca gcgaggaggc ctgggacgcg gcgatggctc tcgccctcct
     4021 ccccgccccg gacccgggcg cggtggtggt gatctcggac ggtggcggcc acgcgaccat
     4081 cgtggccgac accgccgaca aggtcggcct ggcggtcccg ccgctctccg ccgcgactcg
     4141 cgaggccctg gccgcggtgc tgccacagcg gagtgcgatc gggaacccgg tcgacttcgc
     4201 gggagtggcc gaggaggagc cggaggtcgt gccgcgggtg gtggacatcg cgctcggcga
     4261 tcccgccatc ggcggcgccc tcatcgcggg ccacttcggc ggctacgtca agatcgccac
     4321 cgaggagctc ggccggcgcg aatgcgcggc ggcgctggag ctgacgcggg tggtggcgcg
     4381 gcggggcaag ccggtcatcg tgcacaccat ctacggcgcg gagcccctgc ccgcgctcga
     4441 gcccctgcgg gcggccggcg tgccgatcta tcgctcgctc gaggcctccg cccgcgcgat
     4501 gggcgcgctg tggcaacacg cccgggcgca gcgggcgcgc gccggcgccg ggccgcgccg
     4561 ctcgcagccg gatgcggcgc gcgtgtcggc gctcctggcc ggcgcgcgcc ccggagaggt
     4621 gctgccagag cccgagtgcc gggagctcgt ggcgtgctac ggcattccgg tgccgccctg
     4681 gcaggtggtg accggcgcgg aggagacggc ggccgctgtc gaagccctcg ccgcccccgc
     4741 cgcgctcaag ctggtggccc gcggcgtcgt gcacaagagc gacgtcggcg gcgtgctcct
     4801 cggaatccgc ggggcggccg cggcgcgcga cggctgggcg cgcctcatgg acgcggcggc
     4861 gcgggccggc gccaccgacg cccgggtgct cgtcaccccg atgctcgacc ccggcgtgga
     4921 ggcggtggtc ggcgtgctcc gcgaccggca gttcggcccg gtggtgatgt tcgggctggg
     4981 cggcgtgctc gtcgaggcgc tcgacgacgt ggtgttccgg gtggccccgt tcggcgcggg
     5041 cgaggcggaa gcgatgatct ccgagatcca cgggcaccgg ctgttcggcc cggtgcggag
     5101 acgccccccg gtggatcgca cagcgctggt ggacctgctc gtccgcgtct cggagctcgc
     5161 ctcggaccga ccggagctgg ccgagctcga cctgaacccg gtgatctgct cggcgcgcgg
     5221 cgcggccctc gcggacgcgc gcgcggtggt gggctgacgt gcggcgggcc gcgcgcaccc
     5281 ggctctccac cgcgctgctg ctcgcgccgc cgct
//
LOCUS       DATMCV010000802         7885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_41470, whole genome shotgun sequence.
ACCESSION   DATMCV010000802 DATMCV010000000
VERSION     DATMCV010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41470"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..882
                     /gene="pgeF"
                     /locus_tag="VNU03_18365"
     CDS             127..882
                     /gene="pgeF"
                     /locus_tag="VNU03_18365"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWP76490.1"
                     /translation="MTSRAPRDPDALSHFVTSRLLDRHGLPHLFTTRHFPGIARPTES
                     RPPLGPESRALLATRGLVGEPVFARQVHGADVIRVEHGGFAGHGDALATSRAGLPLCI
                     STADCLPIVLYDPRAGRLAAVHAGWRGTVQSIGRAAVSALAEAGSAPRDLVVAIGPSI
                     GPCCYEVDAPVLERLETAFPGAWEPWAMPKGPGKWMLDLWRANVDQLTAAGVPPEGID
                     LLGLCTGCRPDLLFSYRRERGAGRLVTVAALPG"
     gene            911..1600
                     /locus_tag="VNU03_18370"
     CDS             911..1600
                     /locus_tag="VNU03_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWP76491.1"
                     /translation="MLDMRANLGRVQEAVARACARAGRSPDHVLLIAVSKTMEVERVR
                     LAIAAGVAALGENRVQEAKEKIEALGRPVPWHLIGSLQTNKARDAARLFDWIHSVDRL
                     DLAQELSRRAHGAQRPLNVLLQVNLGDEPQKGGVAPAELKRLHEMVAGLPALKVRGLM
                     AIPPATEQPEQARPYFRRLRELRDELALEHCSMGMSADFEVAIEEGATMVRVGTAIFG
                     ARAPRGQERTG"
     gene            1597..2418
                     /gene="proC"
                     /locus_tag="VNU03_18375"
     CDS             1597..2418
                     /gene="proC"
                     /locus_tag="VNU03_18375"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWP76492.1"
                     /translation="MSIKDKRVGFLGAGNMGEAIIKGLLQTGLVPASAIAATDVRADR
                     LQQLASQYGIGAASSNHDLVAKSDVVILAVKPQIMGAVLKEIAGAVDRTRLLISVAAG
                     VPVARLREGLGKDARLIRVMPNTPALVLEGVTAIARADGLEAGDLELAQELFGAVGRA
                     VVLDEDALDAVTGLSGSGPAYVAIVIESLADGGVKMGLDRATAMILAAQTVLGSARLI
                     LETGMHPGQLKDMVSSPGGTTIAGIAALEEGGVRRTFIGAVERATLRSRELGGKR"
     gene            2418..2690
                     /locus_tag="VNU03_18380"
     CDS             2418..2690
                     /locus_tag="VNU03_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWP76493.1"
                     /translation="MPELIGFFGWVLGLYSWVIIAAALITWVSPDPRNPVVMFLRQVT
                     EPVLAPVRRLLPPWKTGGLDFSPLIVILAIQFVERVILPGLLRAMY"
     gene            2701..3177
                     /locus_tag="VNU03_18385"
     CDS             2701..3177
                     /locus_tag="VNU03_18385"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HWP76494.1"
                     /translation="MRITPMDIRQQQFTVRMFRGFDVQEVDTFLEDLAADYEALLKEN
                     TLLKEHLQAAEERARGLEQRERMLQETLVTTQRMVEEMKDNARREAQLLIKEAEMQGE
                     KTIEAARTAEGAIQAQISQLKRTRRQLAESLRATVDMYQRLLDQDLEADANQGDNG"
     gene            3203..3469
                     /locus_tag="VNU03_18390"
     CDS             3203..3469
                     /locus_tag="VNU03_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HWP76495.1"
                     /translation="MQPRARSNEVLGWQGDTLRVRVTAPPAHGEANRAVTDLLAEALG
                     VAASRIALVRGAASRDKLFRVERLSPSDLRARLSLPLPSGERAG"
     gene            3514..4530
                     /gene="mtnA"
                     /locus_tag="VNU03_18395"
     CDS             3514..4530
                     /gene="mtnA"
                     /locus_tag="VNU03_18395"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HWP76496.1"
                     /translation="MITPVRWEDGRLVLIDQTRLPTEEVERACGSWPEVAEAIRSLVV
                     RGAPAIGVAAAFGVVLAARASRATDRSALLADLEEAIKGLAATRPTAVNLFWALDRMR
                     RTAAAGPELSVGALRDRLLVEAQAILAEDLAANRAMGAHGATLVPEHARLLTHCNAGA
                     LATAGYGTAVGVIRAAHELGRLALVWVDETRPVMQGSRLTAWELAKEGIPHRLISDVA
                     AGFVMKRGEVDLVVTGADRIAANGDTANKIGTYSVAVLARHHGIPFYIAAPCSTIDPS
                     IPTGASIVIEERDAAEVRGVAGRQTAPTASPVFNPAFDVTPAELITAIITERGVVRPP
                     YHFT"
     gene            4604..6088
                     /locus_tag="VNU03_18400"
     CDS             4604..6088
                     /locus_tag="VNU03_18400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HWP76497.1"
                     /translation="MRRSGHTLDWETKPLPFKIYPALEAGRLPTGLPVPAIDAFAVLR
                     PSAPITRPAALDLDRLAAILFFSAGVMRVKTYPGGAQVHFRAAASTGALYQTEVYVVA
                     GAVEGLAPGIYHFNPGDFSLRTLRAGDWRGALALAAADDAIAAQPASVVLSAIYWRNT
                     WKYEARGYRHLFWDSGTMLAELLAVARALELPARLVTGFVDAQVNGLLGLDAAKEGAL
                     VIAPIGAEGASAPASPVVTPLALDVVPLSPREVDYPLLRQAYDDSALDSEPEVADWRD
                     RGQVLHSDNVVMQDLTPLGAPRAEAGRTVGETIARRGSTREFSGEAITMEALSSVLYH
                     ATGEIPSDAPAGLVDLYVNVHAVDGLEPGAYVYDLRAHALARLRAGDVRRSSAFLCLD
                     QALGGTSSATVFFLGDLARTLERWGNRGYRLANLEAGLIGGRLYLGAYAQGFGATGLT
                     FYDRAVVDFFSPHASGRDALFVTALGRSRPRSQAGGSLQGIARF"
     gene            6143..7282
                     /locus_tag="VNU03_18405"
     CDS             6143..7282
                     /locus_tag="VNU03_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWP76498.1"
                     /translation="MKKYYLMAPGPTPVPSNVLLAMAQPMIHHRTPEYEALFIEVRAG
                     LKRLFQTSQDVVPFTSSGTGAMEAAVVNTLSAGDTVLALRAGKFGERWEEICRGYGVT
                     VIPLPAPFGETVPAEAFARALREHPEAKAVLMQQSESSTGVLHDVKGIAAVTRASGAI
                     LVVDAVSSLGIADLRMDEWGVDLVVSGSQKGLMLPPGLSFCALSEKAWGHVKASRLPK
                     YYFDLATERKFVARNECRFTPAVSIFVGLREVLKMLEAEGLPAVFRRHERLARATRSG
                     AEALGLGLFAKASPSPALTAVVAPRGLDSEAVLSTYSTSHNITIAGGQGEMKGKVFRL
                     GHMGYVAEFDVITALAALEQVLHELGQPVDFGAGVRAAQKVFAER"
     gene            7288..>7885
                     /locus_tag="VNU03_18410"
     CDS             7288..>7885
                     /locus_tag="VNU03_18410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWP76499.1"
                     /translation="MRMLLVRHAETDWNRERRFQGGRDVSLSATGREQAESAARLLAA
                     TPLDAVWSSPLARARDTAAIIAAPHRLAVQEAAAFREMGFGEWEGLTRDEVRERFADA
                     HRAWVETPQEATWSGAEDLAAVRSRALAGLAALRTAHQGQTICLVTHGITGRLLILEA
                     LGLGLDRLWTFHLSSTGISELEFRDDWTTLHRMNSLIHL"
BASE COUNT         1037 a         2856 c         2873 g         1119 t
ORIGIN      
        1 gccttcctgc ggcggcaggc ggactgagga gcttccccaa gaagctttcc ccctcaccct
       61 gccctctccc ccgtcgaggg gggagagggt gggccgccgc cggaagaggc gtgagacaat
      121 gttggagtga cttcccgcgc cccgcgcgac cccgacgctc tttcgcattt cgtcacctcc
      181 cggctcctcg atcggcacgg gctgccccac ctgttcacga cgcggcactt ccccggcatc
      241 gcgcggccca ccgaatcgcg gcctccgctc ggccccgagt cgcgcgcgct gctggccacc
      301 cggggcctcg tcggcgagcc cgtcttcgcg cgccaggtgc acggggccga cgtgatccgg
      361 gtggagcacg ggggcttcgc gggccacggg gacgcgctgg ccaccagtcg ggccgggctg
      421 ccgctctgca tctcgaccgc cgactgcctg cccatcgtcc tctatgaccc gcgtgccggg
      481 cggctggccg cggtgcacgc gggctggcgg ggcaccgtgc agtcgatcgg ccgcgccgcg
      541 gtctcggccc tggcggaggc cggcagcgcg ccccgcgacc tggtagtggc catcggcccg
      601 tccatcgggc cctgctgcta cgaggtggac gcccccgtgc tggagcgcct ggagacggcc
      661 tttccagggg cgtgggagcc ctgggcgatg cccaagggcc ccggcaagtg gatgctcgac
      721 ctctggcggg ccaacgtgga ccagctcacc gccgccggcg tccccccgga aggtatcgat
      781 ctgctggggc tctgcaccgg ctgccggccc gacctcctct tctcctaccg ccgggagcgc
      841 ggtgccgggc gcctcgtgac ggtcgcggcg ctccccggat agggcgggcg tgctagaatc
      901 gcccccactc atgctggaca tgcgcgccaa cctcgggcgg gtccaggagg ccgtcgcgcg
      961 ggcgtgcgcg cgggccggtc gctcccccga ccacgtcctg ctcatcgcgg tctccaagac
     1021 catggaagtg gagcgggtac ggctggccat cgccgccggc gtcgccgctc tcggggagaa
     1081 ccgcgtccag gaggcgaagg agaagatcga ggcgctcggg cgcccggtgc cctggcactt
     1141 gatcgggtcg ctgcagacca acaaggcgag ggacgcggcc cgcctgttcg actggatcca
     1201 ctcggtggac cggctcgatc tggcgcagga gctgtcgcgg agggcgcacg gggcgcagcg
     1261 gccgctcaac gtgctgctcc aggtgaacct gggggacgag ccgcagaagg gcggcgtcgc
     1321 ccccgccgag ctgaagcggc tgcacgagat ggtggcgggg ctgcccgctc tgaaggtgcg
     1381 cggcctcatg gcgatcccgc ccgcgacgga gcagccggag caggcgcggc cgtacttccg
     1441 gcgcctgcgc gagctgcgtg acgagctggc cctcgagcac tgctcgatgg gcatgagcgc
     1501 cgacttcgag gtggccatcg aggaaggcgc gaccatggtg cgggtgggga ccgcgatctt
     1561 cggcgcgcgg gctccgcgcg gacaggagcg gaccgggtga gcatcaagga caagcgggtc
     1621 ggcttcctcg gcgcggggaa catgggcgag gccatcatca agggcctgct ccagaccggg
     1681 ctcgtgcccg cctccgcgat cgcggccacc gacgtgcgcg ccgaccggct ccagcagctc
     1741 gcatcccagt acggcatcgg cgcggcctcc tccaaccatg atctggtcgc caagtccgac
     1801 gtggttatcc tggccgtgaa gccgcagatc atgggcgcgg tgctgaagga gatcgcgggc
     1861 gcggtggatc gtacccggct cctgatctcg gtggcggcgg gggtgccggt ggcgcggctg
     1921 cgcgaggggc tcggcaagga cgcgcgcctg atccgggtga tgccgaacac gcccgcgctg
     1981 gtgctcgagg gcgtgaccgc gatcgcgcgc gccgacggcc tcgaggcggg cgatctggag
     2041 ctggcgcagg agctgttcgg agcggtcggc cgcgcagtgg tgctcgacga ggacgccctc
     2101 gacgcggtga ccgggctctc cggctcgggc cccgcctacg tggccatcgt catcgagtcg
     2161 ctcgccgacg gcggcgtgaa gatggggctc gaccgcgcca ccgccatgat cctggccgcc
     2221 cagaccgtgc tcggctcggc gcgcctcatc ctggaaaccg ggatgcaccc gggacaattg
     2281 aaggacatgg tgtcctcgcc gggcggcacc accatcgccg ggatcgcggc gctggaagag
     2341 ggcggggtgc ggcggacctt catcggcgcg gtcgagcggg cgaccctgcg ctcgcgcgag
     2401 ctgggaggca aacgctagtg ccggagctca tcggtttctt cggctgggtg ctggggctct
     2461 actcgtgggt gatcatcgcg gcggccctca tcacctgggt cagccccgat ccccgcaatc
     2521 ccgtcgtgat gttcctgcgg caggtcaccg agccggtgct ggctccggtg cgccggttgc
     2581 ttccgccgtg gaagaccggg gggctcgatt tctcccccct gatcgtcatc ctggccatcc
     2641 agttcgtgga gcgggtgatc ctgcccggcc tgctccgagc gatgtactga ggagagtccc
     2701 gtgcgcatca cgcccatgga tatccgtcag cagcagttca ccgtccggat gttccgcggc
     2761 ttcgacgtcc aggaggtgga caccttcctc gaggatctcg ccgccgacta cgaggcgctc
     2821 ctgaaggaga acacgctcct gaaggagcac ctgcaggcgg ccgaagagcg cgcgcgcggg
     2881 ctcgagcagc gcgagcggat gctgcaggag accctggtga ccacccagcg catggtcgag
     2941 gagatgaagg acaacgcgcg ccgcgaggcc cagctcctga tcaaggaagc ggagatgcag
     3001 ggggagaaga ccatcgaggc cgcccgcacc gccgagggcg ccatccaggc ccagatcagc
     3061 cagctcaagc ggacccggcg ccagctcgcg gagtcgctgc gggccaccgt cgacatgtac
     3121 cagcggctgc tggatcagga cctcgaggcg gatgccaacc agggcgacaa cggctagcgc
     3181 tcccccgctg ctgcacgtcc gggtgcagcc ccgcgcccgg agcaacgagg tgctgggctg
     3241 gcagggcgac accctgcgag tgcgggtgac cgcgccgcct gctcacgggg aggccaatcg
     3301 cgcggtgacc gacctgctgg ccgaggcgct cggcgtcgcc gcctcgcgga tcgcgctggt
     3361 gcgcggcgcc gcctcccgcg acaagctctt ccgcgtcgag cgcctctctc catccgacct
     3421 ccgcgcccgc ctctctctcc ctctcccctc gggggagagg gcagggtgag gggtccagga
     3481 tgaggggcgc agggtgaggg gcgtgggccc gcgatgatca cccccgtccg ctgggaggac
     3541 ggccgcctgg tcctgatcga ccagacccgc ctccccaccg aggaggtcga gcgcgcctgc
     3601 gggagctggc ccgaggtcgc ggaggccatc cgcagcctgg tcgtccgcgg cgctcccgcg
     3661 atcggggtgg ccgccgcgtt cggggtggtg ctcgccgcgc gggccagccg ggcgaccgac
     3721 cgctcggctc tgctcgcgga tctggaggaa gcgatcaagg gtctggccgc gacgcggccc
     3781 accgcggtca acctcttctg ggctctcgac cggatgcggc ggactgctgc ggccggcccc
     3841 gagctctcgg tgggcgcgct gcgggaccgg ctgctcgtcg aggcccaggc catcctggcc
     3901 gaggacctcg cggccaaccg ggcgatgggc gcccacggcg ccacgctcgt gcccgagcac
     3961 gcgcgtctgc tcacccactg caacgcgggc gcgctggcca ccgcgggcta cggcaccgcg
     4021 gtcggggtga tccgcgcggc gcacgagctc ggccggctcg cgctggtgtg ggtggacgag
     4081 acgcggccgg tcatgcaggg ctcccggctg accgcgtggg agctggccaa ggagggcatc
     4141 ccgcaccggc tcatctccga cgtagcggcg ggcttcgtga tgaagcgcgg cgaggtcgac
     4201 ctggtcgtga ccggcgccga ccgcatcgcg gccaacggcg acaccgccaa caagatcggg
     4261 acctactcgg tggcggtgct cgcgcgccac cacggcatcc cgttctacat cgcggcgccg
     4321 tgctcgacca tcgacccgtc gatcccgacc ggcgcctcca tcgtgatcga ggagcgcgac
     4381 gcggccgagg tgcgcggggt ggccgggcgc cagaccgcgc cgacggcctc gccggtgttc
     4441 aaccccgcct tcgacgtgac gcccgccgag ctgatcaccg cgatcatcac cgagcgcggg
     4501 gtcgtccgcc cgccgtacca cttcacgtaa ggtcatctcg aacaaccgcg acatcagggc
     4561 cgcccgcgag taccacgagc gcaccgcgca ttcccccgag tcggtgcgtc ggagcggcca
     4621 caccctcgac tgggagacca agccgctccc gttcaagatc taccccgcgc tcgaggcggg
     4681 gcgcctgccc accgggctgc cggtgcccgc gatcgacgcc ttcgccgtgt tgcgcccatc
     4741 cgcaccgatc acccggccgg ccgcgctcga tctcgaccgg ctcgccgcga tcctcttctt
     4801 ctcggccggc gtcatgcgcg tcaagacgta cccggggggc gcccaggtcc acttccgggc
     4861 ggccgcctcc accggcgcgc tctaccagac cgaggtgtac gtggtggccg gggcggtgga
     4921 gggcctcgcg cccgggatct atcacttcaa cccgggcgac ttcagcctga gaacgctgcg
     4981 ggccggcgac tggcgcggtg ccctcgccct ggccgcggcc gacgacgcga tcgcggccca
     5041 gcccgcctcg gtggtcctct ccgcgatcta ctggcgcaat acctggaagt acgaggcgcg
     5101 cggctaccgg cacctcttct gggactcggg caccatgctg gccgagctgc tggcggtggc
     5161 gcgcgcgctg gagctgccgg cccgcctcgt caccggcttc gtggacgcgc aggtcaacgg
     5221 attgctcggg ctcgacgccg cgaaggaggg cgcgctggtg atcgcgccga tcggcgccga
     5281 gggggcgtcc gcccccgcgt ccccggtggt cacgccgctc gcgctcgacg tggtcccgct
     5341 ctccccgcgc gaggtcgact acccgctcct ccgccaggcc tacgacgact ccgccctcga
     5401 ctccgagccc gaggtcgccg actggcgcga ccggggtcag gtcttacatt ccgacaatgt
     5461 cgtaatgcaa gacctgaccc cgctgggggc gccgcgggcc gaggcggggc ggacggtggg
     5521 agagacgatc gcccggcgcg gatccacgcg cgagttctcc ggcgaggcga tcacgatgga
     5581 ggcgctgtcg agcgtgctct atcacgcgac gggggagatc ccgtccgacg cgccggccgg
     5641 gctggtcgat ctgtacgtca acgtgcacgc ggtggacggg ctcgagccag gcgcctacgt
     5701 gtacgacctc cgcgcgcacg cgctcgcgcg gctccgggcc ggcgacgtgc gccggagctc
     5761 cgcgttcctc tgcctcgacc aggcgctcgg cggcacgtcg agtgccaccg tcttcttcct
     5821 gggcgatctg gcgcgcacgc tcgagcgctg gggcaaccgg ggctaccgcc tggcgaacct
     5881 cgaggcgggg ctgatcggag ggcgcctcta cctgggcgcc tacgcccagg gcttcggggc
     5941 gaccggcctc acgttctacg accgggcggt ggtcgacttc ttctccccgc atgcgagcgg
     6001 ccgggacgcg ctcttcgtca cggccttggg ccgctcgcgc ccccgttcac aagccggcgg
     6061 ctcgttgcaa ggtatcgcac ggttctgata ccgtggtcgg gtcaactcga ccctctggga
     6121 gcttgccctc aaggagccgg caatgaagaa gtactacctg atggccccgg gcccgacccc
     6181 cgtgccctcg aacgtcctgc tggcgatggc ccagccgatg atccaccacc ggacgccgga
     6241 gtacgaggcg ctcttcatcg aggtccgggc ggggctcaag cgcctcttcc agacgagcca
     6301 ggacgtggtc ccgttcacct cgtccgggac cggggcgatg gaggccgcgg tggtcaacac
     6361 cctgtcggcc ggcgacaccg tcctggcgct gcgggcgggg aagttcggcg agcgctggga
     6421 ggagatctgc cgcggctacg gggtgaccgt gatcccgctc ccggccccct tcggcgagac
     6481 ggtcccggcg gaggccttcg cgcgcgcgct gcgcgagcac cccgaggcga aggcggtgct
     6541 gatgcagcag agcgagtcct cgaccggcgt gctccacgac gtgaagggca tcgccgcggt
     6601 cacccgcgcc tccggggcga tcctggtggt cgacgcggtg tcgagcctcg gcatcgccga
     6661 tctccggatg gacgagtggg gcgtggacct ggtggtgtcg ggctcccaga aggggctgat
     6721 gctgcccccg gggctctcgt tctgcgcgct ctccgagaag gcgtggggcc acgtgaaggc
     6781 ctcgcgcctg cccaagtact acttcgacct cgccaccgag cggaaattcg tggcgcggaa
     6841 cgaatgccgc ttcacccccg ccgtctccat cttcgtgggg ctgcgggagg tgctcaagat
     6901 gctcgaggcc gaggggctcc cggcggtgtt ccgccgtcac gagcgcctgg cccgggccac
     6961 ccggtcgggc gccgaggccc tgggcctcgg gctgttcgcc aaggcgtcgc cgagccccgc
     7021 gctgaccgcg gtggtggcgc cgcgcggcct cgacagcgag gcggtgctct cgacctactc
     7081 gacctcgcac aacatcacga tcgcgggcgg gcagggggag atgaagggca aggtgttccg
     7141 cctgggccac atgggctacg tggccgagtt cgacgtgatc acggccctgg ccgcgctcga
     7201 gcaggtcctg cacgagctcg ggcagccggt cgacttcggc gccggggtcc gcgcggccca
     7261 gaaggtcttc gccgagcgct gatcgccatg cgcatgctgc tggtgcgtca cgccgagacc
     7321 gactggaacc gggagcgtcg cttccagggc gggcgagacg tctcgctgtc ggccaccggc
     7381 cgcgagcagg cggagtcggc cgcgcgcctg ctggccgcca ctccgctcga cgcggtgtgg
     7441 tcgagcccgc tcgcgcgcgc ccgcgacacc gcggccatca tcgcggcccc gcaccgcctc
     7501 gcggtccagg aggcggccgc cttccgcgag atgggcttcg gcgagtggga gggcctcacg
     7561 cgcgacgagg tgcgggagcg cttcgcggac gcccaccggg cctgggtcga gaccccgcag
     7621 gaggcgacct ggtccggcgc cgaggacctt gcagcggtgc ggagccgggc gctcgcgggc
     7681 ctggccgcgc tccggaccgc ccaccagggc cagaccattt gtctcgtcac ccacgggatc
     7741 accggccgcc tgctgatcct ggaggcgctc ggcctggggc tcgatcggct ctggacgttc
     7801 catctctcgt cgaccgggat ctcggagctc gagttccgcg acgactggac gacgctgcac
     7861 cgcatgaact cgctgatcca cctgg
//
LOCUS       DATMCV010000803         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_642510, whole genome shotgun sequence.
ACCESSION   DATMCV010000803 DATMCV010000000
VERSION     DATMCV010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642510"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VNU03_18415"
     CDS             complement(<1..685)
                     /locus_tag="VNU03_18415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWP76500.1"
                     /translation="MPISSRRLGRWAHRHARWLFPAPAVLLVAAIIVYPILYTVWMSL
                     QEWSASSLIGPRFIGMANYHKIALADARFQEAVGRTIYFTIVAVGAETLLGLAMALLF
                     NREFWGRGLLRTLAILPMVATPTAIALVFVMMYHPSLGVANYLLSVVGLSPWRWTYSS
                     QTALYALALVDVWQWTPLIMLIALAGLASLPREPYEAAHIDGASTLRAFWHITLPLLR
                     PALVVAVLFR"
     gene            complement(696..2015)
                     /locus_tag="VNU03_18420"
     CDS             complement(696..2015)
                     /locus_tag="VNU03_18420"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWP76501.1"
                     /translation="MPARRLTRRAFLAVAAAGAAAGPFVFTPARAQGFNWKRFQGKEL
                     FLLLTKHPFVDVLEKNIPEFESLSGMKVKWETLPEIQARQKMTVEMTANSGGIDAFFT
                     SLHVEKKRFWKAGWYHPLNKFLQDATLTSPDFDWNDFSPGARAGVTQPDGSVSALAAF
                     IDANVLFYRKDLFASKNVTPPKTLAELEPLVQKLHAPPGTYGIVLRGLKNANATQYPS
                     ILFPMGGTYLKDGKAALDTKEQVAAIDLYSKLLRQYGPPGVVNFNWYECSASFMQGQV
                     AIYMDGVNFASQFEDPAKSKVVGKVGYAMLPSGPGGHFSPIYITGMAVNAQSRNKEAS
                     YLFCQWATNKANAVRELMAGVGVGRTSTWDNPEVKAKPKMPADWYAAYQASLKIGRQG
                     LPEIVAVTEYRDIIGVAIQKAIEGAPPAQVLAQAQKEFQDMLNRTEG"
     gene            complement(2139..3359)
                     /locus_tag="VNU03_18425"
     CDS             complement(2139..3359)
                     /locus_tag="VNU03_18425"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP76502.1"
                     /translation="MRQIADDRRLPADVEAQAPVQAAAALRPVIRAYHDELEREQRLP
                     KALVEQFHAAGFYRLVRPRELGGLQTDPLTYLRTVELLAEGSGSVGWNLCNNNIAQLV
                     TLGLPDGGIREIYGPGGDVAVAGTAVQGGGRAEPVDGGYRVTGRWPFGTGCQESPWML
                     GSFQILDGDQPRRSPDGASVYWRGVFHRAETQIVAGSWDVSGLRATGSFDWTVDDVFL
                     PERRTMVHAGVPLDNQWSHWPGVSYALPAQVWVGPHHSAVITGIARAGIDALIELAVE
                     KTPRGRTSRLCDNPQVQDAIGRADAILNAGRAYRSATITEVWNTLAAGEDTTLEQRAR
                     CRLAAVHAADCAREAMDLVYRQGGSTSYRRESRLAECWGDLQVVGQAVTIMPEWYPMG
                     GRVFMKMDPGPRLR"
BASE COUNT          541 a         1178 c         1256 g          550 t
ORIGIN      
        1 cgcggaacag gaccgccacc accagcgcgg gcctgagtag cgggagcgtg atgtgccaga
       61 acgcccgcag cgtcgaggcg ccgtcgatgt gggcggcctc gtaaggctcg cggggcaggc
      121 tcgccaggcc ggccagcgcg atcagcatga tgagcggcgt ccactgccac acgtcgacca
      181 gggcgagcgc gtacagcgcg gtctggctcg agtacgtcca ccgccacggc gacagcccca
      241 ccacggagag caggtagttc gccaccccga ggctcgggtg gtacatcatc acgaagacga
      301 gcgcgatggc ggtcggcgtc gccaccatgg gcaggatggc cagggtgcgc agcaggcctc
      361 gcccccagaa ctcgcggttg aacagcagcg ccatcgcgag accgaggagg gtctcggcgc
      421 cgacggccac gatcgtgaag taaatcgtcc gccccaccgc ctcctggaag cgcgcgtccg
      481 cgagcgcgat cttgtggtag ttcgccatcc cgatgaagcg cggcccgatc aggctggagg
      541 cggaccactc ctggaggctc atccacaccg tgtagaggat cgggtacacg ataatcgccg
      601 cgacgaggag caccgcgggc gcggggaaca gccaccgggc gtggcgatgc gcccagcgcc
      661 cgagccggcg ggaggagatc ggcacccccc agccgctagc cctccgtccg gttgagcatg
      721 tcctggaact ccttctgcgc ctgcgccagg acctgcgcgg gcggcgcgcc ctcgatggcc
      781 ttctggatcg ccaccccgat gatgtcgcgg tactcggtga ccgcgacgat ctcgggcagc
      841 ccctgccggc cgatcttgag cgaggcctga taggccgcgt accagtcggc gggcattttg
      901 ggcttggcct tgacctcggg gttgtcccag gtggaggtgc ggcccacccc gaccccggcc
      961 atgagctcgc gcaccgcgtt cgccttgttg gtggcccact ggcagaagag gtacgaggct
     1021 tccttgttcc ggctctgggc gttgaccgcc atgcccgtga tgtagatcgg cgagaagtgc
     1081 ccgccggggc cggagggcag catcgcgtag ccgaccttgc cgaccacctt ggacttggcc
     1141 ggatcctcga actggctcgc gaagttcacg ccgtccatgt agatcgcgac ctggccctgc
     1201 atgaaggacg ccgagcactc gtaccagttg aagttgacca cgccgggcgg cccgtactgc
     1261 cggaggagct tgctgtagag gtcgatggcc gcgacctgct ccttggtgtc gagcgcggcc
     1321 ttgccgtcct tcagataggt gccgcccatc gggaacagga tgctcggata ctgcgtcgcg
     1381 ttggcgttct tgagcccgcg gaggacgatg ccgtaggtgc cgggcggggc gtggagcttc
     1441 tgcaccagcg gctcgagctc ggcgagcgtc ttcggcggcg tgacgttctt ggacgcgaag
     1501 aggtctttcc ggtagaacag cacgttcgcg tcgatgaagg ccgcgagcgc ggagacgctg
     1561 ccgtcgggct gggtcacccc ggcgcgggcg cccggggaga agtcgttcca gtcgaagtcg
     1621 gggctggtca gggtcgcgtc ctggagaaac ttgttcagcg ggtggtacca gcccgccttc
     1681 cagaagcgct tcttctcgac gtggagcgag gtgaagaagg cgtcgatccc acccgagttc
     1741 gccgtcatct cgaccgtcat cttctggcgg gcctggatct cgggcagggt ctcccacttg
     1801 accttcatcc cggagagcga ctcgaactcg gggatgttct tctcgagcac gtcgacgaac
     1861 ggatgcttgg tcaggagcag gaagagctcc ttgccctgga agcgcttcca gttgaagccc
     1921 tgtgcgcggg cgggggtgaa gacgaagggg cctgcggcgg cgccggcggc agcgacggcg
     1981 agaaaggcgc ggcgggtcag tcggcgagcg ggcatctcgg gctcctctcg tgcgataggc
     2041 ggcattgcct tcggcgggcc gtggcgggag agcgagccgt ggcgacgcgc gccgatcctg
     2101 tcagagcgcg ggggacaagt caaaccgggc cgcggtcgct agcgcagccg cgggcccggg
     2161 tccatcttca tgaagacccg gccgcccatc gggtaccact ccggcatgat ggtgaccgcc
     2221 tgaccgacga cctggagatc gccccagcac tcggccagac ggctctcgcg ccggtaggac
     2281 gtgctgccac cctgccggta caccaggtcc atcgcctcgc gggcgcagtc ggcggcgtgg
     2341 acggcggcca ggcggcaccg ggcgcgctgc tccagcgtgg tgtcttctcc ggccgcgagc
     2401 gtgttccaca cttccgtgat cgtcgcgctg cgatacgcgc ggcccgcgtt caggatcgcg
     2461 tcggcccggc cgatcgcgtc ctgcacttgc gggttgtcgc agagacgact ggtccggccg
     2521 cgtggcgtct tctcgacggc cagctcgatc agcgcgtcga tgccggcccg ggcgatgccg
     2581 gtgatcaccg cgctgtgatg tggcccgacc cagacctgcg cgggcagcgc gtacgagact
     2641 cccggccagt ggctccactg gttgtcgagt gggaccccgg cgtggaccat cgttcgccgc
     2701 tccggcagaa agacgtcgtc gaccgtccag tcgaagctgc cggtcgcacg gagcccggac
     2761 acgtcccagc ttcccgcgac gatctgcgtc tcggcgcgat ggaagacgcc gcgccagtag
     2821 accgaggcgc catccggact ccggcgcggc tggtcgccat cgaggatctg gaagctgccc
     2881 agcatccagg ggctctcctg gcacccggtg ccgaacggcc agcgaccggt gacccggtag
     2941 ccaccgtcga ctggttccgc ccggccaccg ccctgcacgg cggtgccggc gacggcgacg
     3001 tcaccgccgg gcccgtagat ctcccggatg cctccgtccg gcaagccgag cgtgaccagc
     3061 tgggcgatat tgttgttgca gaggttccag ccgaccgagc cggaaccctc ggctagcagc
     3121 tcgaccgtcc gcagatacgt gagcgggtcg gtctgcaggc cgcccagctc ccgcggtctc
     3181 acgagccggt agaagccggc cgcgtggaac tgctcgacca gcgccttcgg caagcgctgc
     3241 tcgcgctcga gctcgtcgtg atacgcgcgg atgactggcc gcagcgcggc cgcggcctgg
     3301 actggggcct gagcctcgac gtcagccggt aggcgtctgt cgtcggcaat ctgtcgcatt
     3361 ggccgggatg ttccgctggc ggagcagagg tgtcaagagg tacagtcgct gatcatcccc
     3421 agccaggaga cctgatgaca gacaagattc gcgtgggcat cgtgggagcg accgtgacgc
     3481 aagggggcag tggctggggg gcgaacgctc acgttcccgc cttga
//
LOCUS       DATMCV010000804         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_642522, whole genome shotgun sequence.
ACCESSION   DATMCV010000804 DATMCV010000000
VERSION     DATMCV010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642522"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VNU03_18430"
     CDS             <1..1002
                     /locus_tag="VNU03_18430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP76503.1"
                     /translation="TAITREPVQVPDITVPGAYESRIRDNLIASGIRAVLAMPMAHHD
                     RLVGCIAVTRNRPGDFPVETIDLLRTFAAQSALAIQNARLFTEIEDKSRQLEAASRHK
                     SEFLANMSHELRTPLNAVIGFSEVLIQRMFGELTDKQEEYLKDIFSSGQHLLSLINDI
                     LDLSKIEAGRMELAISEFDLPGAIENVLILMRERAGRRGVTLGRTVDERVGTIHADER
                     KVKQVLLNLLSNALKFTPEGGRIDVRAGFRDGVVEIAVTDTGIGIAPEDHEAVFEEFR
                     QVGHASLGVEGTGLGLALSRKFVALHGGRIWVESAIGRGATFTFTLPPTPVPSPPRA"
     gene            complement(1038..1640)
                     /locus_tag="VNU03_18435"
     CDS             complement(1038..1640)
                     /locus_tag="VNU03_18435"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HWP76504.1"
                     /translation="MTDRADPFDWSGGHPALDLVNTLDERPSDSPIEKLVSYRDLVRF
                     AGRAGLITTSMSARLLRLDGRSCARVVERTRELRERFHDVLAAAHSRRRPRRRDLDAI
                     SAAVQAAHAAQVLVASPARGLAHHEWSPASVPDIPLHACSLAIERLVLEAKPARVRKC
                     GASDCDVYYLDTSKSRLRQWCRMTSCGNREKQRRWRAATR"
     gene            1692..>2180
                     /locus_tag="VNU03_18440"
     CDS             1692..>2180
                     /locus_tag="VNU03_18440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWP76505.1"
                     /translation="MQRGYSTSVEYTQGALYGLAAVGIWSGWIVVARLGLRTSLTPWD
                     IAALRFSVAGLLLLPFVLKKGLDVERLGGVGLAALVLGGGAPVLLANVGLLFAPAAHA
                     GALFPGVMPLMVAILAAVVLREAFTGTKKIGFALILPGVFGIAWGAGGAGGARENIGH
                     ALF"
BASE COUNT          331 a          768 c          724 g          357 t
ORIGIN      
        1 accgcgatca cccgggagcc ggtgcaggtg cccgacatca cggtgccggg cgcgtacgag
       61 agccggatcc gggacaacct gatcgcctcc gggatccggg ccgtgctggc gatgccgatg
      121 gcccaccacg accgcctcgt cggctgcatc gcggtcaccc gcaaccgtcc cggcgacttc
      181 ccggtcgaga cgatcgatct gctgcgcacg tttgccgccc agtcggccct cgcaatccag
      241 aacgcgcgcc tgttcaccga gatcgaggac aagagccggc agctcgaggc ggcgagccgt
      301 cacaagtccg agttcctcgc caacatgtcg cacgagctcc ggactcccct caacgcggtc
      361 atcgggttct ccgaggtgct catccagcgg atgttcggcg agctgaccga caagcaggag
      421 gagtacctca aggacatctt ctcctccggc cagcacctgc tctcgctgat caacgacatc
      481 ctggaccttt cgaagatcga ggccgggcgc atggagctgg ccatcagcga gttcgatctg
      541 ccgggggcca tcgagaacgt cctgatcctg atgcgggagc gggccggccg ccgcggcgtc
      601 acgctcggcc gcaccgtcga cgagcgggtc gggacgatcc acgccgacga gcggaaggtg
      661 aagcaggtgc tcctcaacct gctctccaac gcgctgaagt tcactccgga gggcggacgg
      721 atcgacgtgc gcgccggttt ccgtgatggc gtggtcgaga tcgcggtgac cgacaccggc
      781 atcggcatcg cccccgagga ccacgaggcg gtcttcgagg agttccgtca ggtgggccac
      841 gcgagcctcg gggtggaggg caccggcctc gggctggccc tgtcgcggaa gttcgtcgcg
      901 ctccacggcg gccggatctg ggtggagagc gcgatcggtc gcggcgcgac cttcaccttc
      961 acgctcccgc ccaccccggt cccgtccccg ccccgcgcgt agtcgagctc gtcgcccggc
     1021 ctcaccccgt cacgccgcta tcgcgtcgcg gcgcgccatc gccgctgctt ctcccggttt
     1081 ccgcagctcg tcatgcggca ccactgccga agtcggctct tgctcgtgtc gagatagtag
     1141 acgtcgcagt cggaggcgcc gcacttccgg actctcgcgg gcttcgcctc gaggaccaga
     1201 cgctcgatgg ccagcgaaca ggcatgcagt gggatgtcgg gcaccgaggc cggagaccac
     1261 tcgtgatggg cgagcccccg ggccggcgat gcgaccagca cctgcgccgc atgcgcggcc
     1321 tgcaccgcgg cggatatcgc gtccagatca cgccgccggg gccgccggcg cgagtgggcc
     1381 gcggccaaca catcgtggaa acgctcgcgc agctcacggg tgcgctccac gacgcgcgcg
     1441 caagagcgcc cgtcgagacg aagcagtcgc gccgacatcg aggtcgtgat gagccccgcc
     1501 cgtccggcga accgaaccag atcccggtag ctgaccagct tctcgatcgg cgaatcggag
     1561 ggccgctcat ccagggtgtt caccaggtcc agggccggat gccccccgct ccaatcgaat
     1621 gggtccgccc gatcggtcat tctcactctt gtcttcggtc gtgtcctgtt gatatcataa
     1681 cccttaaaac aatgcaaaga ggttattcaa cttccgtcga gtatacccag ggcgcactct
     1741 acggcctcgc cgcggtcggc atctggtcgg gatggatcgt ggtggctcgc ctcggtctcc
     1801 ggacgagtct caccccgtgg gacatcgcgg cgcttcgatt cagcgtagcc ggattgctcc
     1861 tgctgccgtt cgtactgaag aagggcctcg acgtcgagcg cctcggcggg gtcggactgg
     1921 cggcgctcgt gctcggcggc ggagctccgg tcctgctcgc gaacgtgggc ctgctcttcg
     1981 ctcccgccgc gcacgcggga gccctcttcc ccggggtgat gccgctgatg gtcgcgattc
     2041 tcgccgcggt cgtcctgcgc gaggcattca ccggaacgaa gaagatcggc ttcgcgctga
     2101 tcctccccgg cgtctttggc atcgcttggg gcgccggcgg cgccggcggg gccagggaga
     2161 atatcggcca cgcgctgttt
//
LOCUS       DATMCV010000805         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_310605, whole genome shotgun sequence.
ACCESSION   DATMCV010000805 DATMCV010000000
VERSION     DATMCV010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310605"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VNU03_18445"
     CDS             complement(<1..958)
                     /locus_tag="VNU03_18445"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWP76506.1"
                     /translation="MKELLTADINERYAVALNYRQIDRFMIQVLWWHFIAIGLLTLGN
                     TVFRLGLYFPSPFSWRVPTLEEGAVTIGLGLLASAIPAGLYGRVRNHYAWRVLLSASF
                     TAFSYLFVFISGGSIEMHFHFFMIMALLVMYSDWRLGWFVLVLTALHHAVLNYLQPNW
                     VYFYGRNDFAIVAHGLPVGVMALFTTRLCQIQRQSVAILEETRRVQEEDLLKRREAEE
                     TLRESEEKSRYVIESAADAIVSIDAEGAVREFNRAAERLFGYTAAEMAGEPLTRLIPE
                     RFHEAHRADLRRSLESGERRLRSWRNLDLVGLTQDRREVPLEM"
     gene            complement(955..1473)
                     /locus_tag="VNU03_18450"
     CDS             complement(955..1473)
                     /locus_tag="VNU03_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76507.1"
                     /translation="MWPIWFAGVLLLAGMPGVMGRALAHGAPEKVVDERFVVTVALAP
                     DGDLTRLRFFFRDFKTGRGLTGPMSVRVRIAEDETPTPLHDGRAAVVGSWADVLYQFP
                     RDGFYEVVLEFSLDGEPARVYRPEDWRIWVGKPRPLNIRAWAWAAATSTLLTMVIVVS
                     IKRQRTERGGAR"
     gene            complement(1476..>2133)
                     /locus_tag="VNU03_18455"
     CDS             complement(1476..>2133)
                     /locus_tag="VNU03_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP76508.1"
                     /translation="TTADAGRTPVLVTVTDTGAGMTDEVRRRCLEPFFTTKGERGTGL
                     GLAMAYGVIQRHRGTFDIASQPGQGTTFRIRLPARSDRSVAAQPSSASAPSRPLRVLV
                     VDDEPQMRQVMSECLVSDGHAVETAPNGRVGLRRFESGRFDVVVTDRAMPEMNGLELA
                     AAIQQTALRKPVVILLSGFGDELDTAGGPPAGVDLVVGKPVTLEKLRDMLAMAVAPGD
                     "
BASE COUNT          354 a          770 c          699 g          310 t
ORIGIN      
        1 acatctccag cggcacctcc cgtcggtcct gcgtcaaccc gaccaggtcg aggttccgcc
       61 agctgcggag ccggcgctcg ccgctctcga gggaccggcg caggtcggcc cgatgcgcct
      121 cgtggaagcg ctccggaatc aggcgcgtca ggggctcccc ggccatctcg gccgcggtgt
      181 agccgaacag ccgctcggcc gcccggttga actcgcggac cgctccttcc gcgtcgatcg
      241 acacgattgc gtcggcggcc gactcgatca cgtaccgcga cttctcctcg ctctcgcgca
      301 gcgtctcctc ggcctcacgg cgcttgagca gatcttcctc ctggacgcgt cgcgtctctt
      361 cgaggatcgc aaccgactgg cgctggatct ggcagaggcg cgtcgtgaac agtgccatca
      421 ccccgacggg caggccgtgc gccacgatcg cgaaatcgtt ccgaccgtag aagtagaccc
      481 agttcggctg gaggtagttg aggacggcgt ggtgcagcgc ggtcagcacg aggacgaacc
      541 agccgagccg ccagtccgag tacatcacga gaagagccat gatcatgaag aagtggaagt
      601 gcatctcgat cgagccaccg ctgatgaaca cgaacaggta cgaaaaagcg gtgaaggagg
      661 cgctcaacag gacccgccag gcgtagtggt tccgtacgcg gccgtacaag ccggcgggga
      721 tcgcgctggc caggaggccg agcccgatgg tcaccgcgcc ctcctcgagc gtggggaccc
      781 gccacgagaa gggggacggg aagtagagtc ccagccggaa cacggtgttg cccagcgtga
      841 ggagcccgat ggcgatgaag tgccaccaga gcacctggat catgaatcga tcgatctgcc
      901 ggtagttgag ggccaccgcg tagcgctcgt tgatgtcggc ggtcagaagc tccttcacct
      961 cgcacctccc cgttcggtcc gctggcgctt gatggatacg acgatcacca tggtcaacag
     1021 ggtcgacgtc gccgccgccc aggcccaggc ccggatgttc agcggccgcg gcttgccgac
     1081 ccagatccgc cagtcctccg gccggtacac gcgtgccggc tcaccgtcga gggagaactc
     1141 gaggaccacc tcatagaacc cgtcgcgcgg gaactggtag agcacgtccg cccagctgcc
     1201 caccaccgcc gcgcgcccgt cgtggagcgg cgtgggtgtc tcgtcctccg cgatgcggac
     1261 tcgaaccgac atcggcccgg tcagccctcg cccggtcttg aaatctcgga agaagaaccg
     1321 cagccgcgtg aggtctccgt ccggagcgag ggcgaccgtg acgacgaatc gctcgtcgac
     1381 caccttctcc ggcgccccgt gggccagcgc gcggcccatg accccgggca tcccggccag
     1441 cagcagcacg cccgcgaacc agatgggcca cacggtcagt cgccgggcgc cacggccatg
     1501 gcgagcatgt cccgcagctt ctcgagggtc accggcttcc ccaccacgag gtcaacgccg
     1561 gccggcgggc ccccggccgt atcgagctcg tcgccgaatc cactcagcag aatgaccacc
     1621 ggcttccgga gtgccgtctg ctggatggcc gcggccagct ccagtccgtt catctcgggc
     1681 atcgcgcgat cggtgaccac gacgtcgaac cggccgctct caaaccgccg cagacccact
     1741 cgcccgttcg gagcggtctc gaccgcgtgc ccgtcactca ccaggcactc actcatcacc
     1801 tgccgcatct gcggctcgtc gtccacgacg aggacccgaa gggggcggga cggcgcgctc
     1861 gccgagctcg gctgggcggc gaccgatcga tccgagcgcg cgggcaaccg gatgcggaac
     1921 gtcgtgccct ggccgggctg gctcgcgatg tcgaatgtgc cgcggtggcg ctggatgacc
     1981 ccgtacgcca tcgccagccc caggcccgtt ccccgctcgc ccttggtcgt gaagaacggc
     2041 tcgaggcacc gccggcgcac ctcgtcggtc attcccgcgc cggtgtccgt gaccgtcacg
     2101 agcaccggcg tacggccggc gtccgcggtg gtc
//
LOCUS       DATMCV010000806         6501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_380042, whole genome shotgun sequence.
ACCESSION   DATMCV010000806 DATMCV010000000
VERSION     DATMCV010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380042"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VNU03_18460"
     CDS             complement(<1..442)
                     /locus_tag="VNU03_18460"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWP76509.1"
                     /translation="MQILKGLPSFPPELRPSVAALGAFDGIHLAHAKILDTAVARARA
                     LGVHAVACTFDPHPTVVLRPALAPPPIATLEENLARIARHGLDAALVIPFTRELSLME
                     AEAFVDDVLVRTLGTREVVVGFNHTFGAGARGTAALLLELGRLRG"
     gene            complement(405..1322)
                     /gene="truB"
                     /locus_tag="VNU03_18465"
     CDS             complement(405..1322)
                     /gene="truB"
                     /locus_tag="VNU03_18465"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWP76510.1"
                     /translation="MSDRHRAGILPVEKGPGVTSFQVVAHLRRVLRAPRIGHGGTLDP
                     AATGLLPILIGEATKLTPYLVDLDKEYVATVRLGVTTESQDLSGAVLETRPVPPLDAA
                     AIEAALAPLVGVIQQVPPMFSALRRGGKRLYELARQGVEVDREPREVTVHSIRLMSMS
                     LPDFVIHVRCGKGTYVRTLAADAGAALGCGAALAGLVRTRVGPFALDSAVTWDEVRQA
                     RSGETLWPRLLPPDAALAGLPPVRLDADGARAFAHGQAVAAGGRVLGWLRVYGPDDAL
                     LGVGQGLGDRVKPERLLHADPQGSSVLPA"
     gene            complement(1319..2329)
                     /locus_tag="VNU03_18470"
     CDS             complement(1319..2329)
                     /locus_tag="VNU03_18470"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HWP76511.1"
                     /translation="MSDPTPAGITVVSEQPPAQLLDALRRPRGAALMLGHVHPDGDVL
                     GTLLGLGLAMEEAGWSVSYGGPDPVPAVLDFIPGGDRWQVWRTAPRRFDTIVLTDCPN
                     DGRTEGLLAGVRGPESQVLNIDHHPDNRRYGTVNWIDPTAAATGEMIHDLLVALGWPI
                     RPETALGLYTAVHTDTGSFRYSNTTPRTFRIAAALTAAGAEPALVTDRLYQRRPKDAL
                     AILGSLLGRVEMSDDGRVATLTVPEGAASEEFMAAEDLVTYPRSIAGVKVAVLLRAEP
                     DGRVKASLRGKGDVPVNRIAHRFGGGGHENAAGCTLPGPLAAAKTALLAAVREALDGG
                     AP"
     gene            complement(2331..2699)
                     /gene="rbfA"
                     /locus_tag="VNU03_18475"
     CDS             complement(2331..2699)
                     /gene="rbfA"
                     /locus_tag="VNU03_18475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWP76512.1"
                     /translation="MQAKRLERVNQLIKEEISMLVQRQLKDPRLGFVTVTEVDTSPDL
                     KHAKVYFSVLGPEDKWAASFKALESARGFVWSWLRRHLDLRTTPELLFRPDRSMEHAA
                     HIQALLADLRSKAPDSPEGE"
     gene            complement(2759..3049)
                     /locus_tag="VNU03_18480"
     CDS             complement(2759..3049)
                     /locus_tag="VNU03_18480"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWP76513.1"
                     /translation="MSGARVAVGTVELHLPDIGSLKGKRHALKGLKDTLRRRFEISVA
                     EVDHHDVWQRATLALACVSGDSRHANEVISKAMDYIEDHVDGWVTDVQVEIL"
     gene            complement(3062..5599)
                     /gene="infB"
                     /locus_tag="VNU03_18485"
     CDS             complement(3062..5599)
                     /gene="infB"
                     /locus_tag="VNU03_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWP76514.1"
                     /translation="MAARVKVIDLAKELGVTSKDLIVALEGMGHKGMRAMSPLLAAQA
                     NELRVKLGRGRELPAEAKPKRAPKPKAAEGDAVAAAPKKKAAGKKAAGKVAEDAAAMV
                     ELAATPGPELAKPAATIFRPAPAPLSPPVAPEITPAPPIAPAATVTSPPAPAAPGAPP
                     RPAAVPAPAQPARPAVVSPAPAIVRPAPAAPPRPMSPPSPVRPAVAPAAAPRPAAAPV
                     PPRPAAPPIPARPAAAAVAAPAATPATPVRSESLPIPPPPEPPQEIKRELIKLPESVT
                     VGELAAGMRRKSTEVIKALLELGVMATVNEVLDPTAAKLVADRFHFDVEVRSLEGDLL
                     EEEESDPAQMRTRPPVVTVMGHVDHGKTSLLDAIRTTKVAEREFGGITQHIGAYQVDT
                     SHGKVTFLDTPGHEAFTAMRARGAQATDMVILVVAADDGVMPQTVEAINHAKAANVPI
                     LVAVNKIDKPGSDPDRVKRELANQGLVPEDWGGQTIFIHTSAKRGDGIPQLLEMTALQ
                     AEILELRANPGRSGRGVIVEGRLDRGRGPVATALIQSGTLREGDAVVVGSHAGRVRAM
                     FNDRGKKVASAGPSDPVEILGLSGVPQAGDTMLVVSDERKARQIATVRSERDRLKGKG
                     ATRITLEDLHKQIAAGEVKELRLVIKADVQGSVEALTESLERLSTDEVKLKVIHSSVG
                     GVTESDVMLASASNAIVLGFNVKADPKATSQAQANGVDMRSYNVIYDAINDVRAALSG
                     MLAPEIREVVLGRAQVRQLFPISKVGTIYGSSVTEGKMVRGARARIKRGDTVLGESTV
                     NSLKRFKDDVREVLQGLECGIGIEGIKGVQPNDVIEAFSTEEVARTL"
     gene            complement(5606..5842)
                     /locus_tag="VNU03_18490"
     CDS             complement(5606..5842)
                     /locus_tag="VNU03_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HWP76515.1"
                     /translation="MRPQAELIRLVRGEAGDVRVDREGIAAGRGAYVCATVECMEKAL
                     QAGRLGHAFKRPSRPPQESPVVILASWLASWKGR"
     gene            complement(5890..>6501)
                     /locus_tag="VNU03_18495"
     CDS             complement(5890..>6501)
                     /locus_tag="VNU03_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWP76516.1"
                     /translation="GLRGTRIQVISRELRGEKIDIVEWSPDPVVFVARALSPAKVSSV
                     TVEERPEGEEGDDLRSVLVVVPDNQLSLAIGKKGQNARLAAKLTGFRVDIKSEGEIEE
                     ERRRDEEERAEGRQTLVHLPGVGPQLVEKLVEVGLHSPARIVAAGLPALQALPGVGET
                     KASSILAAATAWIAEHPAPVAAEPSAAVEGDGGSEVEPSPLER"
BASE COUNT          826 a         2387 c         2354 g          934 t
ORIGIN      
        1 agccgcggag gcggcccagc tccaggagca gcgccgcggt gccgcgggcc ccggccccga
       61 aggtgtggtt gaagcccacc accacctcgc gcgtgccgag cgtgcggacg agcacgtcgt
      121 cgacgaaggc ctcggcctcc atcagcgaga gctcgcgggt gaacgggatg acgagcgcgg
      181 catcgagccc gtgccgggcg atgcgggcca agttctcctc gagggtcgcg atcggcgggg
      241 gcgccagcgc cggccggagc accacggtcg gatgcggatc gaacgtgcac gcgaccgcgt
      301 gcactcccag cgcgcgcgcg cgggccaccg cggtgtcgag gatcttggca tgggccaggt
      361 ggatgccgtc gaacgcgccg agcgcggcca ccgagggccg cagctcaggc gggaaggacg
      421 gaagaccctt gaggatctgc atgcagcagt cgttcaggct tcacccgatc gccgagcccc
      481 tgccccaccc cgagcagtgc atcgtcgggg ccgtacacgc ggagccagcc cagcacccgg
      541 ccgcccgcgg ccaccgcctg gccgtgcgcg aacgcccggg ccccgtccgc atccaggcgc
      601 accgggggca gccccgcgag cgccgcgtcg ggcggcagga gccgcggcca cagggtctcg
      661 ccgctgcggg cctggcgcac ctcgtcccag gtgacggcgg agtcgagcgc gaacggtccg
      721 acccgcgtgc ggacgaggcc ggcgagcgcg gcgccgcagc cgagggccgc gccggcgtcg
      781 gccgcgagcg tgcgcacgta ggtgcccttg ccgcagcgga catggatcac gaagtcgggc
      841 aggctcatcg acatcaggcg gatcgagtgc acggtgacct cccgcggctc gcggtctacc
      901 tcgacgccct gccgggccag ctcgtagagc cgcttgccgc cccggcgcag ggccgagaac
      961 atcggcggca cctgctggat caccccgacg agcggcgcaa gcgcggcctc gatcgcggcc
     1021 gcgtcgagcg gcggcaccgg ccgggtctcg agcaccgcgc cggagagatc ctggctctcg
     1081 gtggtcacgc ccaggcgcac ggtggccacg tactccttgt cgaggtccac caggtacggg
     1141 gtgagcttgg tggcctcgcc gatcaggatc gggaggaggc cggtcgcggc gggatcgagc
     1201 gtgccgccgt ggccgatgcg cggagcgcga aggacccgcc gcaggtgggc caccacctgg
     1261 aacgacgtga ccccggggcc cttctcgacc ggcaggatgc cggcgcgatg gcgatcgctc
     1321 acggcgcgcc gccgtcgagg gcctcgcgga ccgcggccag cagcgcggtc ttcgccgcgg
     1381 cgagcgggcc cggcagggtg cacccggccg cgttctcgtg gccaccgccg ccgaagcgat
     1441 gcgcgatacg attcaccggc acgtcgccct tgccccggag gctcgccttc acccgcccgt
     1501 ccggctcggc ccgcaggagg accgcgacct tcacgcccgc gatcgagcgt gggtaggtca
     1561 cgagatcctc ggcggccatg aactcctcgg aggccgcacc ctcgggcacc gtgagcgtgg
     1621 ccacccgtcc gtcgtcgctc atctccacgc ggccgagcag gctccccagg atcgcgagcg
     1681 cgtccttcgg tcgtcgctgg tagagccgat cggtgacgag cgcgggctcg gctcccgccg
     1741 cggtgagggc ggccgcgatc cggaaggtgc gcggagtggt gttggagtag cggaacgagc
     1801 cggtatcggt gtggaccgcg gtgtagaggc cgagggcggt ctcggggcgg atcggccacc
     1861 cgagggcgac gagcagatcg tgaatcatct cgccggtggc ggccgcggtc gggtcgatcc
     1921 agttgacggt gccgtagcgg cgattgtccg ggtggtggtc gatgttgagc acctggctct
     1981 ccggcccgcg caccccggcc aggagcccct cggtgcgccc gtcgttcggg caatcggtga
     2041 gcacgatggt gtcgaagcgc cgcggcgcgg tccgccacac ctgccagcgg tcgccgccgg
     2101 gaatgaagtc gagcaccgcc ggcaccggat cgggcccccc gtagctgacg ctccagccgg
     2161 cctcctccat ggcgaggccc aggcccagca gcgtgccgag gacgtcgcca tcgggatgga
     2221 cgtggcccag catgagcgcc gcgccgcggg gccggcggag cgcgtcgagc agctgcgcgg
     2281 ggggctgctc ggagaccacg gtgatgccgg cgggggtggg gtcgctcacg tcactcgccc
     2341 tccggggagt cgggcgcctt cgagcgcaga tcggccagga gggcctggat gtgcgcggcg
     2401 tgctccatgg atcggtcggg gcggaagagc agctccgggg tcgtgcgcag gtcgaggtgg
     2461 cggcggagcc agctccacac gaagccgcgc gcgctctcga gggccttgaa cgaggcggcc
     2521 cacttgtcct ccgggccgag gaccgagaag tacaccttcg cgtgcttgag gtccgggctc
     2581 gtgtccacct cggtgacggt gacgaagccg aggcgcgggt ccttgagctg acgctggacg
     2641 agcatcgaga tctcttcctt gatcagctga ttcacgcgct ccagacgctt agcttgcatg
     2701 acgccggtag cacctttctt gtattcgctc gtctcgcctt cggctgcgcc tcgccctgct
     2761 agagaatctc gacctgcacg tcggtgaccc agccgtcgac gtggtcctcg atgtagtcca
     2821 tggccttgga gatcacctcg ttggcgtggc gcgagtcgcc ggacacgcac gcgagcgcca
     2881 gggtcgcgcg ctgccacacg tcgtggtggt cgacctcggc gaccgagatc tcgaagcggc
     2941 ggcgcagggt gtccttgagt cccttcaggg cgtggcgctt gcccttgagc gatccgatgt
     3001 ccggcaggtg cagctcgacg gtgccgaccg ccacccgcgc tcccgacact ggtcgacgcc
     3061 cctacagggt gcgggcgacc tcttcggtgg agaaggcctc gatgacgtcg ttgggctgca
     3121 cgcccttgat gccctcgatg ccgatgccgc actcgagccc ctgcagcacc tcgcgcacgt
     3181 cgtccttgaa ccgcttgagc gagttgacgg tgctctcgcc cagcacggtg tcgccgcgct
     3241 tgatgcgggc ccgcgccccg cggaccatct tgccctcggt gaccgacgag ccgtagatcg
     3301 tgcccacctt ggagatcggg aagagctgcc gcacctgcgc ccgccccagc acgacctcgc
     3361 ggatctcggg ggccagcatg ccggacagcg ccgccctcac gtcgttgatc gcgtcgtaga
     3421 tcacgttgta gctgcgcatg tcgacgccgt tggcctgggc ctggctcgtg gccttggggt
     3481 cggccttcac gttgaagccg agcacgatgg cgttggaggc ggaggcgagc atcacgtccg
     3541 attcggtcac cccgcccacc gagctgtgga tgaccttgag cttgacctcg tcggtggaca
     3601 ggcgctcgag cgactcggtg agggcctcga ccgagccctg cacgtccgcc ttgatgacca
     3661 ggcgcagctc cttgacctcg cccgccgcga tctgcttgtg caggtcctcg agggtgatcc
     3721 gggtcgcgcc cttgcccttc agacggtcgc gctcgctgcg cacggtggcg atctgccgcg
     3781 ccttgcgctc gtcggagacc accagcatgg tgtcgcccgc ctgcggcaca cccgagaggc
     3841 ccaggatctc gaccgggtcc gacgggcccg cgctcgcgac cttcttgccg cgatcgttga
     3901 acatcgcgcg cacgcggccg gcgtgggagc cgaccaccac cgcgtcgcct tcccgcagcg
     3961 tgcccgactg gatgagggcg gtggccaccg gaccgcgccc gcgatcgagc cggccttcca
     4021 cgattacccc gcgcccggac cgcccggggt tggcgcgcag ctcgaggatc tccgcctgca
     4081 gcgcggtcat ctcgaggagc tgcgggatgc cgtcgccccg cttggccgag gtgtggatga
     4141 agatggtctg gccgccccag tcttccggca ccaggccctg gttggcgagc tcgcgcttga
     4201 cccggtccgg atcggagccc ggcttgtcga tcttgttcac cgcgaccagg atcggcacgt
     4261 tggccgcctt ggcgtggttg atcgcctcca ccgtctgcgg catcaccccg tcgtcggccg
     4321 cgaccaccag gatcaccatg tcggtggcct gcgccccgcg ggcccgcatc gcggtgaagg
     4381 cctcgtggcc gggggtgtcg aggaaggtga ccttgccgtg gctcgtgtcg acctggtacg
     4441 cgccgatgtg ctgggtgatg ccgccgaact cgcgctccgc caccttggtg gtgcggatcg
     4501 cgtcgagcag cgacgtcttg ccgtggtcga cgtggcccat gaccgtgacc accggcggcc
     4561 gcgtgcgcat ctgcgccggg tcggactctt cctcctccag cagatcgccc tcgagggagc
     4621 ggacctccac gtcgaagtgg aaccggtccg cgacgagctt ggccgcggtc gggtcgagca
     4681 cctcgttgac ggtggccatg acgcccagct cgagcagcgc cttgatcacc tcggtcgatt
     4741 tgcgccgcat tccggccgcg agctcgccga ccgtgaccga ctccggcagc ttgatcagct
     4801 cacgcttgat ctcctgcggc ggctcggggg gcggcgggat cgggagcgac tccgaacgta
     4861 ccggcgtggc gggcgtcgcc gcgggcgcgg ccacagctgc agcggctggc cgagccggga
     4921 tcggcggcgc ggcggggcgc gggggcaccg gggcggcggc cgggcgtggc gccgcggccg
     4981 gcgccaccgc gggacgaacc ggcgacggcg gagacatcgg gcgcggcggg gccgcgggag
     5041 ccggacgcac gatcgcgggc gcggggctca ccaccgcggg ccgcgcgggc tgcgccggcg
     5101 cgggtacggc cgccggccgg ggcggcgccc cgggagcggc gggcgccggc ggagacgtca
     5161 ccgtggcggc cggcgcgatc gggggcgcgg gggtgatctc gggcgccacc ggtggagaca
     5221 gtggggcggg cgcgggacgg aagatcgtcg ccgcgggctt ggccagctcg ggccccggcg
     5281 tcgccgccag ctcgaccatt gccgccgcat cctcggcaac cttgccggct gccttcttgc
     5341 cggcggcctt cttcttgggt gccgccgcga ccgcgtcccc ctccgccgcc ttgggcttgg
     5401 gagcgcgctt gggcttcgcc tccgcgggca gctcgcgccc gcgcccgagc ttgacccgca
     5461 gctcgttggc ctgcgcggcc aggagcgggc tcatggcccg catgcccttg tggcccatgc
     5521 cctcgagcgc cacgatcaag tccttgctgg tgacgcccag ctccttcgcc aggtcgatga
     5581 cctttacccg tgccgccacg cgcgattacc tccccttcca gctcgcgagc cagctcgcga
     5641 gaatgactac cggactctcc tgcggcggcc ggctcggccg cttgaacgcg tggcccagtc
     5701 gtcccgcctg cagcgccttc tccatgcatt cgacggtggc gcacacgtac gcgccccgtc
     5761 ccgccgcgat cccctcacga tccactcgta cgtcgcccgc ctcgccccgt accagccgga
     5821 tcagctccgc ctgcggccgc accgtccggc acgcgatgca ggtccgctcc ggccgaccgc
     5881 cccgcccgct cagcgctcga gcgggctcgg ctcgacctcc gaccccccat cgccctcgac
     5941 cgcggcggac ggctccgcgg ccaccggggc cggatgctcg gcgatccacg cggtcgcagc
     6001 ggcaaggatc gaggaggcct tcgtctcgcc cacgcccggc agcgcctgca gcgcgggcag
     6061 gcctgcggcc acgatacgcg ccggcgagtg cagcccgacc tcgaccagct tctccaccag
     6121 ctgcgggccg accccgggca ggtggacgag cgtctggcgg ccctcggcgc gctcttcctc
     6181 gtcgcggcgc cgctcctcct cgatctcgcc ctcgctcttg atgtcgacgc ggaagccggt
     6241 gagcttcgcg gcgagccgcg cgttctgccc cttcttgccg atggcgaggg acagctggtt
     6301 gtcgggcacg accaccagca ccgagcgcag gtcgtcgccc tcctcgccct ccggccgctc
     6361 ctccaccgtg accgaggaga ccttggccgg cgacagggcc cgggccacga agaccaccgg
     6421 gtcgggcgac cactccacga tgtcgatctt ctccccgcgc agctcgcggc tgatgacctg
     6481 gatgcgcgtg ccgcgcagcc c
//
LOCUS       DATMCV010000807         3530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_839350, whole genome shotgun sequence.
ACCESSION   DATMCV010000807 DATMCV010000000
VERSION     DATMCV010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839350"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /gene="ligD"
                     /locus_tag="VNU03_18500"
     CDS             <1..1367
                     /gene="ligD"
                     /locus_tag="VNU03_18500"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWP76517.1"
                     /translation="FGAVHLGLYDGPAGAPRLVYVSKVGTGFDQAGLAAIWERLRPLA
                     RATSPFEAGAVPTGRGHHWVEPRLVCEVRFTDWTEDDGLRHPTFVGLRADRKPEECRR
                     EAPEEMAPVDAAGPGGSAVDAAPVPAAAPEPAPRLKVTNPRKVFWPAEGYTKADLIEY
                     YERVAPLMLPYLRDRPLVLTRFPDGIAGKSFFQKDAPEFAPPWVRTERIYSKDTERDI
                     AYLVVDDVEMLRYVANSASIPIHCWASRVPSLERPDWLVLDLDPKGAPFTDVVRVALA
                     LRGILDGLALPSYVKTSGATGLHILLPLGARYTYEQTRTFARLLAMLGVEALPDISTV
                     ARPLRARGGKVYIDFGQNGHGQTIVAPFSLRPLPGAPASCPLEWAEVTPGLDPAGFNL
                     ATIAERFERMADPLRPVLEDGIDMVAAIERIQARVATAGAVSEAAAPPAGGRTRGRSR
                     PPRA"
     gene            complement(1300..2001)
                     /locus_tag="VNU03_18505"
     CDS             complement(1300..2001)
                     /locus_tag="VNU03_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWP76518.1"
                     /translation="MIRIGTSGYNYPEWRGTFYPEKFPTGKMLPYYAERFSTVEINYT
                     FYRMPNAKTVAGWVAETPAHFSFTLKAPQRITHFARLRDIGDPLRYFLDTAAGLGPKL
                     GTVLFQLPPNFKKDLDRLGGLLDLIPAGLRCAFEFRHDSWLDPAVYDRLRAANAALCV
                     ADTEDATTPIETTADWGYLRLRDQGYTEANLAEWAGKIVALGPTWRDVFVYFKHEEAG
                     TGPAFARQLEALLPR"
     gene            complement(1998..2807)
                     /locus_tag="VNU03_18510"
     CDS             complement(1998..2807)
                     /locus_tag="VNU03_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWP76519.1"
                     /translation="MRIEDPDDPRVADYRAAGRGGADGLFLCEGRLVVRRLLGGSRYR
                     VRSVLATASVLEDLSDALGPAPPPVFVATTAVIRSVFGFNFHRGCLALGERPEQPASP
                     ESVIEPPGPRMVVGLDEVTDPDNVGAIFRNAAAFGVAGVVLSPGCADPLYRKTIRVSM
                     GGTLSTPFARTEWAATLEALRRAGYTVVALTPDPDAETIATAAERLVSRRLAVIVGAE
                     GPGLGEQSRAAADLRVRIPMAPGVDSLNVATACGIALHRLAGGSIMGGGPA"
     gene            2946..>3530
                     /locus_tag="VNU03_18515"
     CDS             2946..>3530
                     /locus_tag="VNU03_18515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWP76520.1"
                     /translation="MERAVFPGFEFPVTARAALLVIDMQPWGVGAEGGLVQAIERTAS
                     GYTRHLTARVRETVIPNTLRLLDAFHRLRRPVYFTAFASATGDGRDVTTATIRYRDTE
                     RRARTGQSVILPRSDPATNVIPELAPRPGDRVLTKTSMDVFTSTRLAEELRVGGIETL
                     VITGVLTDAGVESSARHAAERGFQVIVVDDACAAW"
BASE COUNT          482 a         1236 c         1322 g          490 t
ORIGIN      
        1 acttcggggc ggtgcatctc ggtctctacg acggaccggc cggggcgccg cgcctcgtct
       61 acgtctcgaa ggtgggtacc gggttcgacc aggccggtct cgccgcgatc tgggagcggc
      121 tccgcccgct cgcccgcgcg acgtctccct tcgaggccgg ggcggtgccg accggacgcg
      181 gccaccactg ggtcgagccg cgactggtgt gcgaggtgcg cttcaccgac tggaccgagg
      241 acgacgggct ccgccacccg accttcgtcg gcctgcgcgc ggaccggaag ccggaggagt
      301 gccggcgcga ggcgccggag gagatggcgc cggtcgatgc cgccgggccg ggcggctccg
      361 cggtcgacgc ggctcccgtg ccggccgccg cccccgagcc ggcgccgcgg ctcaaggtca
      421 ccaacccgcg caaggtcttc tggccggcgg agggatacac caaggcggac ctgatcgagt
      481 actacgagcg cgttgcgccc ctcatgctgc cctacctgcg cgaccggccg ctggtgctga
      541 cccgcttccc ggacggcatc gccgggaagt ccttcttcca gaaggacgcg cccgagttcg
      601 cgccgccgtg ggtgcggacc gagcgcatct actcgaagga caccgagcgc gacatcgcgt
      661 acctggtggt ggacgacgtg gagatgctgc gctacgtggc caacagcgcg tcgatcccga
      721 tccactgctg ggcctcacgg gtgccctcgc tcgagcggcc ggattggctc gtgctcgacc
      781 tcgatccgaa gggcgctccg ttcaccgacg tggtgcgggt ggccctcgcc ctgcgcggca
      841 tcctggacgg gctcgccctg ccgagctacg tcaagacgtc gggcgccacc gggctgcaca
      901 tcctgctgcc gctgggcgcg cgctacacct acgagcagac ccgcaccttc gcgcgtctcc
      961 tcgcgatgct gggggtcgag gcgctgcccg acatctccac ggtggcgcgg ccgctgcggg
     1021 cccgtggagg caaggtttac atcgacttcg ggcagaacgg ccacgggcag acgatcgtgg
     1081 cgcccttctc gctgcggccg ctgcccggcg cccccgcctc ctgcccgctc gagtgggccg
     1141 aggtgacgcc ggggctcgac cccgcgggct tcaacctcgc caccatcgcg gagcgcttcg
     1201 agcggatggc cgatccgctg cgcccggtgc tggaggacgg gatcgacatg gtcgcggcca
     1261 tcgagcgcat ccaggctcgg gtggcgacgg cgggagcggt cagcgaggca gcagcgcctc
     1321 cagctggcgg gcgaacgcgg ggccggtccc ggcctcctcg tgcttgaagt agacgaagac
     1381 gtcgcgccac gtgggaccga gcgcgacgat cttgccggcc cactccgcca gattcgcttc
     1441 ggtgtagccc tgatcgcgca gccgcaggta gccccagtcg gcggtggtct cgatcggcgt
     1501 cgtcgcgtcc tcggtgtcgg ccacgcagag ggccgcgttg gccgcgcgca ggcggtcgta
     1561 gaccgcgggg tcgagccagc tgtcgtggcg gaactcgaag gcgcaccgca gcccggcggg
     1621 gatcaggtcg aggaggccgc cgagccggtc caggtccttc ttgaagttgg gcgggagctg
     1681 gaagagcacg gtgccgagct tcggaccgag gcccgccgcg gtgtcgagga agtagcgcag
     1741 cggatcgccg atgtcccgca gccgtgcgaa gtgggtgatc cgctgcgggg ccttgagcgt
     1801 gaaggagaag tgagccggcg tctccgcgac ccatcccgcc accgtcttgg cgttgggcat
     1861 gcggtagaac gtgtagttga tctcgacggt gctgaaccgc tccgcgtagt aggggagcat
     1921 cttgccggtg gggaacttct ccggatagaa ggtgccccgc cactcgggat agttgtagcc
     1981 ggaggtgccg atgcggatca tgcgggtccg ccgcccatga tactgccccc ggccagacgg
     2041 tggagcgcga tgccgcacgc ggtggccacg ttcagcgagt ccacgcccgg cgccatcggg
     2101 atgcgcaccc ggagatcggc cgcggcgcgg ctttgctcgc cgagcccggg gccctccgcg
     2161 cccacgatga ccgcgagccg ccgggagacg agacgctccg cggcggtggc gatcgtctcc
     2221 gcgtccggat ccggggtgag cgcgaccacg gtgtagccgg cccggcggag ggcctcgagc
     2281 gtggcggccc actccgtgcg cgcgaacggg gtggacaggg tcccgcccat cgagacgcgg
     2341 atggtcttgc ggtagagggg atcggcgcag cccggggaga gcaccacgcc cgcgacgccg
     2401 aaggcggccg cgttgcggaa gatggcgccc acgttgtcgg ggtcggtcac ctcgtccagc
     2461 ccgaccacca tccgcggccc gggcggctcg atgacggact cgggggaggc cggctgctcc
     2521 ggccgctcgc cgagcgcgag gcagccccgg tggaaattga agccgaagac ggagcggatc
     2581 accgcggtgg tggccacgaa gaccggtgga ggggcgggcc cgagggcgtc gctcagatcc
     2641 tcgaggacgg atgcggtggc gaggaccgag cgcacccggt agcgggagcc gccgagcagc
     2701 cggcgcacca cgaggcggcc ctcgcagagg aacagcccgt cggctccccc gcgccccgcc
     2761 gcgcggtagt cggcgacgcg cgggtcgtcc ggatcttcga tgcgcaccgg ctgccatgct
     2821 ggcacgggct cccggccttg acaacggaga ggtcggtgtt agcctcccgg ccgtgacaag
     2881 gttgtcatcg acgcgccccg gacacgccac cctgtgggag gtggcccggc ggtcgatcgg
     2941 ctgacgtgga gcgcgccgtc tttcccggct ttgagttccc ggtcactgcg cgggcggccc
     3001 tgctcgtgat cgacatgcag ccgtgggggg tgggggccga gggcggtctc gtgcaggcca
     3061 tcgagcggac cgcctccggc tacacccgcc atctcaccgc gcgcgtccgg gagaccgtga
     3121 tcccgaacac gctccggctc ctggacgcgt ttcaccgcct ccgccgtccc gtctacttca
     3181 ccgcgttcgc gagcgcgacc ggggacggcc gcgacgtgac caccgccacc atccgctacc
     3241 gggacacgga gcgccgagcg cggacgggcc agtcggtgat cctaccgcgc tccgatcccg
     3301 ccaccaacgt gatcccggag ctggcgccgc ggccgggaga tcgcgtgctg accaagacct
     3361 cgatggacgt cttcacctcg acccggctgg ccgaggagct acgggtggga gggatcgaga
     3421 ccctggtcat caccggggtg ctgaccgatg cgggcgtgga gagctcggcc cggcacgcgg
     3481 cggagcgggg atttcaggtg atcgtggtcg acgacgcgtg cgccgcgtgg
//
LOCUS       DATMCV010000808         3830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_509831, whole genome shotgun sequence.
ACCESSION   DATMCV010000808 DATMCV010000000
VERSION     DATMCV010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509831"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNU03_18520"
     CDS             <1..210
                     /locus_tag="VNU03_18520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP76521.1"
                     /translation="TDLGMPDVNGWDVARAVKSGRQGLPVLLLTGWADAEEAGVGRVD
                     AVIKKPFDMTKLAAAVSAALGPASG"
     gene            complement(179..601)
                     /locus_tag="VNU03_18525"
     CDS             complement(179..601)
                     /locus_tag="VNU03_18525"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWP76522.1"
                     /translation="MDARIPSELAAALARARYMYVTTYSRAGNPGTVPTWLWPHDGDV
                     YFTTRRDSLKARRIQNDGRVTVHVGTKDGPAFEGRAEWVDGRADLETALLSDYRRKYW
                     LLVPVFMGRFIRRGLDTQKSVLIRISPDFSRTPDPAPR"
     gene            701..2287
                     /gene="ggt"
                     /locus_tag="VNU03_18530"
     CDS             701..2287
                     /gene="ggt"
                     /locus_tag="VNU03_18530"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWP76523.1"
                     /translation="MGTRGAVASAHPLASMSGIRVLLEGGNAVDAAVAVGSTLNVVEP
                     FMSGAAGIGLMLISRARTRERHVLDFIGPAPRGADPARCTEDELAGGPKSCATPGNLG
                     GWLAALERFGSMPRQKVLAPAIDLAENGVPLTFANVKFFEQSRAQLARSAEAERLFLG
                     NGGPRPGKVVTYKDLAGTLRQVAEGGAEAFYRGPIARTITRAVQEAGGWLAEEDLAAF
                     KPEWREPLDIPYRGREVCSVPPPFSAFQMLETLNILEGFDLAGWGHNSPDYLHHLIEA
                     IKLGSADRLAFAYSGAPPIAGLVSKAYAASQRARIDPARAAASEGERHTRERLAGQIT
                     EGHPAQFVNEQTTHFACADAEGTVVSVTQTLGMAFGSGFAVPGTGLVLNNILKWSDRD
                     PESPNVLKPGRKSGTMMSPTQVFRDGAFELSIGTPGSYGILQTQPQMLLNFVEFGLNL
                     QEAIEAPRIRVYRDRLLDAESRIAEATRAELGRRGHQVNVIDDWSWVVGGGQGIARDP
                     ESGALMAGADPRRDGYALAI"
     gene            2370..3161
                     /locus_tag="VNU03_18535"
     CDS             2370..3161
                     /locus_tag="VNU03_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76524.1"
                     /translation="MDLQDKVAIVTGAGSGIGRGIALGMAARGARVAAVDLDGKSADE
                     TARLLMGAGGKGVGVQADTSRAADVDRAVATVVGQLGPLDVMVNNAGILDGYFNVDET
                     DEAVWTRVIGINLTGVFLGCKRALKEMLPRGAGRIINMASVAGLNGTGGGAAYVASKH
                     GVVGLTRQMAVTYSARGITINAVAPGPILTGLRAHSQEILGPGVPDMSGRGVAVSDEQ
                     VRAIAPAGRRGTVEEIASAVCFLASDEAGYITGHTLVVDGGWRAK"
     gene            3158..>3830
                     /locus_tag="VNU03_18540"
     CDS             3158..>3830
                     /locus_tag="VNU03_18540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP76525.1"
                     /translation="MIKKFSVLYVGQIELENVGINGTPTEQRAYSNERLVEAFQTSKE
                     VAQLMDELGYYCLWTAEHHFQKEGYEVLPNLVQLSLWLATQTRRLKLGCAFNILPMWH
                     PVRLAEDYAMADIVTDGRVIMGVGRGYHTREVESFGAPLLDAAANRELFEEQMDVLLK
                     CFNEESWSHRGKHFVIPPDVDYRGYRLREVTCVPRPIHRPVEIWMPIASGKTIDYMAR
                     MGLKAM"
BASE COUNT          571 a         1332 c         1390 g          537 t
ORIGIN      
        1 accgatctgg ggatgcccga cgtcaacggc tgggacgtgg cgcgcgcggt caagagcggg
       61 cgccaggggc tcccggtcct gctgctgact ggctgggccg acgcggagga ggcgggcgtc
      121 ggccgggtcg acgcggtgat caagaagccg ttcgacatga cgaagctggc cgccgcggtc
      181 agcgcggcgc tgggtccggc gtccggctga agtcgggaga gatccggatc aggacgctct
      241 tctgggtgtc gagccccctg cggatgaagc ggcccatgaa gaccgggacc agtagccagt
      301 acttgcggcg gtagtcggac agcagagcgg tctccagatc cgcgcggccg tccacccatt
      361 ccgctcggcc ctcgaaggcc ggcccatcct tggtgcccac gtggaccgtc acccgaccgt
      421 cgttctggat ccggcgagcc ttgagactgt cgcgccgggt cgtgaagtac acgtcgccgt
      481 cgtgcggcca gagccaggtc ggcacggtgc ccgggttgcc cgcccggctg taggtcgtca
      541 cgtacatgta gcgggcgcgg gcgagggcgg cggccagctc cgaggggatt cttgcgtcca
      601 tgttggctag agcctaccat tgccgggtga accgcagcga accgtgagcc ccggaggaga
      661 ccacatgccc ccgcatcgtc atgcgtaccg tccgaccatc atgggaaccc gcggcgccgt
      721 ggcctccgcg catcccctcg cgtcgatgtc cggcatccgc gtgctgctgg aaggcggcaa
      781 cgcggtcgac gcggcggtgg cggtgggttc caccctgaac gtggtggagc cgttcatgtc
      841 gggtgcggcc gggatcgggc tcatgctgat ctcgcgggcg cgcactcgcg agcggcacgt
      901 gctcgacttc atcggccccg ccccgcgcgg cgccgacccg gcgcgctgca ccgaggacga
      961 gctggcgggc gggccgaagt cgtgcgccac gcccggcaac ctcggcggct ggctcgccgc
     1021 gctcgagcgc ttcgggtcga tgccgcggca gaaggtgctg gcgcccgcca tcgatctggc
     1081 cgagaacgga gtgccgctca ccttcgccaa cgtgaagttc ttcgagcagt cgcgcgccca
     1141 gctggcccgc tcggccgagg cggagcgcct cttcctgggc aacggcgggc cgcgccccgg
     1201 gaaggtcgtc acctacaagg atctggccgg gacgcttcgc caggtggccg aggggggcgc
     1261 ggaggcgttc taccgggggc cgatcgccag gaccatcacg cgtgcggtgc aggaggccgg
     1321 cggctggctc gcggaggagg acctggccgc gttcaagccg gagtggcggg agccgctcga
     1381 catcccatac cggggccggg aggtctgctc ggtgccgccg ccgttctcgg cgttccagat
     1441 gctcgagacc ctcaacatcc tggagggctt cgacctggcc gggtggggcc acaactcccc
     1501 cgactacctc catcacctga tcgaggcgat caagctcggc tcggcggacc ggctggcctt
     1561 cgcctactcg ggggcgcccc cgatcgcggg cctggtgtcc aaggcgtacg cggcctcgca
     1621 gcgggcgcgc atcgacccgg cgcgtgcggc cgcgagcgag ggcgagcggc acacccgcga
     1681 gcggctggcc ggccagatca cggaggggca cccggcgcag ttcgtcaacg agcagacgac
     1741 ccacttcgcg tgcgccgacg cggagggcac cgtggtctcg gtcacccaga cgctcgggat
     1801 ggccttcggc tcgggcttcg cggtgcccgg gaccgggctc gtgctcaaca acatcctcaa
     1861 gtggtcggat cgggatccgg aatcgcccaa cgtgctcaag cccggccgca agtccggcac
     1921 catgatgtcg ccgacccagg tcttccggga cggcgccttc gagctctcga tcggcacccc
     1981 cggctcgtac ggcatcctcc agacgcagcc gcagatgctg ctcaacttcg tcgagttcgg
     2041 cctgaacctg caggaggcca tcgaggcgcc gcggatccgg gtgtaccgcg accggttgct
     2101 cgacgcggag tcgcgcatcg ccgaggcgac ccgtgcggag ctcgggcgac gcggccacca
     2161 ggtcaacgtg atcgacgact ggtcatgggt ggtgggcggc gggcagggga tcgcccgcga
     2221 cccggagtcc ggcgccctca tggccggcgc cgacccccgc cgcgacggct acgccctggc
     2281 catctaagct ggcgccctcg cctcgagggc gccctccaaa tacccctctc ccctccgggg
     2341 agagggcagg gtgaggggaa caaacggcaa tggacctcca agacaaggta gcgatcgtga
     2401 ccggcgcggg ctccggcatc ggccgcggca tcgcgctggg gatggcggca cgcggcgccc
     2461 gcgtggcggc ggtggatctc gacggcaaga gcgcggacga gaccgcgcgc ctgctcatgg
     2521 gcgcgggcgg caagggcgtg ggtgtccagg cggacacgag ccgcgccgcc gacgtggacc
     2581 gcgcggtcgc caccgtggtg ggccagctgg ggccgctcga cgtcatggtc aacaacgcgg
     2641 gcatcctcga cggctacttc aacgtcgacg agaccgacga ggcggtgtgg acccgcgtga
     2701 tcgggatcaa cctgaccggc gtcttcctcg gctgcaagcg cgcgctcaag gagatgctgc
     2761 cccgcggggc cgggcgcatc atcaacatgg cctcggtcgc cgggctcaac ggcaccgggg
     2821 gcggcgcggc ctacgtggcg tccaagcacg gcgtggtcgg cctgacccgc cagatggcgg
     2881 tgacctactc ggcgcgcggc atcacgatca acgcggtggc gcccggcccg atcctgaccg
     2941 ggctccgcgc gcactcgcag gagatcctcg gccccggcgt gccggacatg agcggccgcg
     3001 gcgtggcggt gagcgacgag caggtgcgcg cgatcgcgcc cgcgggccgg cgcgggacgg
     3061 tggaggagat cgcctccgcg gtctgcttcc tggcctcgga cgaggcgggc tacatcaccg
     3121 gacacaccct ggtcgtcgac ggaggctgga gagccaagtg atcaagaaat tctcggtcct
     3181 ctacgtgggg cagatcgagc tggagaacgt cggcatcaac ggcaccccga ccgagcagcg
     3241 cgcctattcc aacgagcgcc tggtcgaggc cttccagacg tcgaaggagg tcgcccagct
     3301 catggacgag ctgggctact actgcctgtg gaccgccgag caccacttcc agaaggaggg
     3361 ttacgaggtg ctgccgaacc tcgtgcagct ctcgctctgg ctcgccaccc agacccggcg
     3421 cctcaagctc ggctgcgcct tcaacatcct gcccatgtgg cacccggtgc gcctcgccga
     3481 ggactacgcg atggccgaca tcgtcaccga cgggcgggtc atcatgggcg tcggtcgcgg
     3541 ctaccacacc cgcgaggtcg agtccttcgg ggcccccctc ctcgacgcgg cggccaaccg
     3601 cgagctgttc gaggagcaga tggacgtgct gctcaagtgc ttcaacgagg agtcgtggag
     3661 ccaccgcggc aagcacttcg tgatccctcc cgacgtggac taccgcgggt accgcctccg
     3721 cgaggtcacg tgcgtgccgc gcccgatcca ccggccggtc gagatctgga tgccgatcgc
     3781 gagcggcaag acgatcgact acatggcccg catgggtctc aaggcgatgg
//
LOCUS       DATMCV010000809         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_839461, whole genome shotgun sequence.
ACCESSION   DATMCV010000809 DATMCV010000000
VERSION     DATMCV010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839461"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2068)
                     /locus_tag="VNU03_18545"
     CDS             complement(<1..>2068)
                     /locus_tag="VNU03_18545"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWP76526.1"
                     /translation="DYIAAARAALGAVPTRDTVVVERFFDEAGGMQMVIHAPFGGRIN
                     RAWGMALRKRLCHSFNFELQAAATDDGVLLSLGPQHSFPLEAVFEMLHPSGLEELLTQ
                     AALQAPMFNTRWRWNANRALALLRWQNGRRVPPYLQRMRAEDLLVAVFPAQLACQDNV
                     GAGPIEVPDHPLVRETLRDCLTEAMDIDGLRACLTAIHGGQIRRVTRETPEPSVFAHE
                     ILNANPYAYLDDAPLEERRARAVTLRRGLPAAVADDIGRLDPEAIAAVVEEAWPDLRS
                     ADELHDLLLDLGALPRDQNDLQTDTRTETWPGYLEELIAAGRAARLRHGDRVLWVAAE
                     RRSLAATVWPDACFEPDVAEPPARRAPAWTDRESALVEIVRARLGLVGPTTAPALAED
                     LGVTPGEVDAALAAVEAEGGVLRGRFTPGAVSGEALAREWCDRRLLARIHRRTLEGLR
                     RLIEPVSPAALLRFLFAWQHVAPGSQPHGRDGLLRVIERLQGFEAAAGAWEREILAAR
                     TSGYQPGWLDELCLSGEVTWGRLGLHESARHAGRRLSATVPLALLLRRDLGWLLDPRP
                     EPGALATAPSGKPSGPALDVLAHLEHAGASFVEDIAAGVRRLRIEVEEGLCELVTAGL
                     VTGDGFAGLRSLLHSEKRRRPRPGRPTPARPSPGTGRWSRLAPPAAPPADDLVEARAR
                     QYVRRYG"
BASE COUNT          255 a          763 c          812 g          238 t
ORIGIN      
        1 ccccgtagcg gcggacgtat tgccgggcgc gggcctcgac caagtcgtcg gcaggcggcg
       61 cggcgggcgg ggcgagccgg gaccagcgcc cggtgccggg cgaggggcgg gcgggggtgg
      121 ggcggccggg gcgcggccgg cgccgcttct cggagtggag caggctgcgg aggccggcga
      181 aaccgtcacc ggtgacgagg cccgcggtca ccagctcgca gagcccttcc tcgacctcga
      241 tgcggagtcg ccgcaccccc gccgcgatgt cctcgacgaa ggaggcgccc gcgtgctcga
      301 gatgggccag cacgtcgagc gcggggcccg agggcttgcc cgacggcgcg gtggccagcg
      361 cgcccggctc cggccgcggg tcgagcagcc agccgaggtc gcgccgcagc agcagggcca
      421 gcggcacggt ggcggagagc cgccggcccg cgtggcgcgc gctctcgtgc aggccgagcc
      481 ggccccacgt gacctcgccg gagaggcaga gctcgtcgag ccagccgggc tggtagccgc
      541 tcgttcgcgc ggccaggatc tcgcgctccc acgcccccgc cgcggcctcg aagccctgca
      601 gccgctcgat cacgcgcagc aggccgtcgc gcccgtgcgg ctggctgccg ggcgccacgt
      661 gctgccacgc gaaaaggaac cgcagcagcg ccgcgggtga caccggctcg atgaggcgtc
      721 ggagcccctc gagcgtccgg cgatggatcc gcgcgagcag gcgccggtcg caccattctc
      781 tcgcgagcgc ttcgccggag accgcgcccg gcgtgaagcg gccgcgcagc acgccgccct
      841 cggcctcgac cgcggcgagc gccgcgtcca cctcgccggg ggtgacgccc aggtcctccg
      901 cgagggcggg ggcggtggtc gggccgacga ggccgagccg cgcgcggacg atctcgacca
      961 gcgcgctctc gcgatcggtc caggcggggg cgcgccgcgc ggggggctcg gccacgtcgg
     1021 gctcgaagca ggcgtccggc cacacggtgg cggccagcga gcggcgctcg gccgccaccc
     1081 agagcacgcg gtcgccgtgg cgcagccgcg cggcgcgccc ggctgcgatc agctcctcga
     1141 gatagccggg ccacgtctcg gtccgggtgt cggtctggag gtcgttctgg tctcggggca
     1201 gcgcgcccag gtcgaggagc aggtcgtgca gctcgtcggc cgagcgcagg tcgggccagg
     1261 cctcctcgac caccgcggcg atcgcctccg ggtccaggcg cccgatgtcg tcggccaccg
     1321 cggcgggcag cccgcgcctc agcgtgaccg cgcgggcccg ccgctcttcg agcggggcgt
     1381 cgtcgagata ggcgtagggg ttggcgttca ggatctcgtg ggcgaacacg gagggctccg
     1441 gcgtctcgcg cgtcacccgc cggatctggc ctccgtggat cgcggtgagg caggcgcgca
     1501 ggccgtcgat gtccatcgcc tcggtcaggc agtcgcgcag ggtctcgcgc accagcgggt
     1561 ggtcgggcac ctcgatcggc cccgccccca cgttgtcctg gcaggcgagc tgggccggga
     1621 acaccgcgac cagcagatcc tccgcgcgca tgcgctgcag atacggcggc acgcgccggc
     1681 cgttctgcca gcgcagcagg gcgagcgccc ggttggcgtt ccagcgccag cgggtgttga
     1741 acatgggcgc ctggagcgcg gcctgggtca gcagctcctc gagcccgctc gggtggagca
     1801 tctcgaagac cgcctcgagc ggaaagctgt gctgggggcc gagggagagc agcacgccgt
     1861 cgtcggtggc cgcggcctgc aactcgaagt tgaagctgtg gcagagccgc ttgcgcagcg
     1921 ccatgcccca cgcgcgattg atgcggccgc cgaagggcgc gtggatcacc atctgcatgc
     1981 cgcccgcctc atcgaagaag cgctccacca ccaccgtgtc gcgcgtcggc accgctccca
     2041 gcgcggcgcg ggccgccgcg atgtagtc
//
LOCUS       DATMCV010000810         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_447422, whole genome shotgun sequence.
ACCESSION   DATMCV010000810 DATMCV010000000
VERSION     DATMCV010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447422"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..888)
                     /locus_tag="VNU03_18550"
     CDS             complement(16..888)
                     /locus_tag="VNU03_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWP76527.1"
                     /translation="MRPAPFDYFAPRTVDEALDLLAHHGEDAKVLAGGQSLVPMMNMR
                     LVRPAVIVDLNRVAALGALREEAGGLRLGALVRQHALERDGRIATTAPLLAEAAPLIG
                     HLQTRARGTIGGSLAHADPAAELPACMIALDAVFHLRSARAPRACRAQDFFRGLLATA
                     LAPDELLAEIEVPGPAAPRTGHGFAEVARRHGDFALVGACAVLALDAAGVCRHARLVI
                     FGAGDGPHLARAADALLGERPSAARLGEIGRAAAGEIDARADLHATPEYRRRVAAGLA
                     ARALGHAAARAEAA"
     gene            complement(899..1804)
                     /locus_tag="VNU03_18555"
     CDS             complement(899..1804)
                     /locus_tag="VNU03_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWP76528.1"
                     /translation="MSTYLPETRYPLELASAMPDVWELWQRAKTLEWDPELDIPWEEL
                     HPERYPEEQIMAARLHWSRRTWGEYGAISESPALLLRFCLENRLPDLRFFFTMRTQEE
                     GRHAEASWLMAERLGGYFQQPQNPPTATAVGTHGVRRMAFDPDTSLEGIFASLVCAAE
                     EILIDAFKATVHKATNPAVRRLMELILRDEVRHIAFGWQCLDAWAPTFTPETVKNIER
                     ATTQMIQNVEFNGYRNLWLAIEGGSATPLEIDADRVACEAGLGGTTPEDEMRIVPQFI
                     KKIRARMAPWGVNLPMFDHPKLGKV"
     gene            complement(1842..>2095)
                     /locus_tag="VNU03_18560"
     CDS             complement(1842..>2095)
                     /locus_tag="VNU03_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76529.1"
                     /translation="GGAVRDMLERVILTGYRNTWLLPAESREPLLAAEALTAEHGLGA
                     STVAQEKAVVRATLAQVRERLATWDVTLAPVDHPELGRA"
BASE COUNT          265 a          746 c          802 g          282 t
ORIGIN      
        1 gtccggggcg atcggtcacg ccgcctccgc ccgcgcggcc gcgtggccga gggcccgcgc
       61 ggcgagcccc gcggcgacgc ggcgccggta ctccggcgtg gcgtgcagat cggcgcgcgc
      121 gtcgatctcg ccggccgcgg cccggccgat ctcgccgagc cgcgccgccg acggccgctc
      181 gccgagcagt gcgtcggcgg cgcgcgccag gtgggggccg tcgcccgcgc cgaagatcac
      241 gaggcgcgcg tggcggcaga cgccggccgc gtcgagcgcg agcaccgcgc acgcgccgac
      301 gagcgcgaag tctccgtgcc ggcgcgcgac ctcggcgaag ccgtgccccg tccggggcgc
      361 ggccgggccg ggcacctcga tctccgcgag cagctcgtcg ggcgcgagcg cggtggccag
      421 gaggccccgg aagaagtcct gcgcgcggca cgctcgcggc gcccgggcgc tccggagatg
      481 gaagacggcg tcgagggcga tcatgcaggc gggcagctcg gcggcggggt cggcgtgggc
      541 caggctgccg ccgatggtgc cgcgagcccg ggtctgcagg tggccgatga gcggcgccgc
      601 ctccgcgaga aggggcgcgg tggtggcgat gcggccgtcg cgctcgaggg cgtgctggcg
      661 aacgagcgcg cccaggcgga ggccgcccgc ctcctcccgc agcgccccga gcgcggccac
      721 gcgattcagg tccacgatca ccgcggggcg caccagccgc atgttcatca tcggcaccag
      781 gctctggccc ccggccagca ccttggcgtc ctcgccgtgg tgcgcgagga ggtcgagggc
      841 ctcgtccacc gttcgcggcg cgaagtagtc gaagggagcg ggccgcacgc gtcttcggct
      901 acaccttgcc gagcttcggg tgatcgaaca tcggcaggtt cacgccccag ggggccatgc
      961 gcgcccggat cttcttgatg aactggggga cgatgcgcat ctcgtcctcg ggggtggtgc
     1021 cgccgaggcc cgcctcgcag gccacgcggt cggcgtcgat ctcgagcggc gtcgcgctgc
     1081 cgccctcgat ggccagccag aggttgcggt agccgttgaa ctccacgttc tggatcatct
     1141 gggtcgtcgc gcgctcgatg ttcttcaccg tctccggcgt gaaggtgggc gcccaggcgt
     1201 ccaggcactg ccacccgaag gcgatgtggc gcacctcgtc gcgcaggatc agctccatga
     1261 gccggcgaac cgccgggttg gtggccttgt gcacggtggc cttgaaggcg tcgatcagga
     1321 tctcctccgc cgcgcagacc aggctcgcga agatgccctc gagcgaggtg tcggggtcga
     1381 aggccatccg acgcaccccg tgcgtgccca ccgcggtcgc ggtcggcggg ttctgcggct
     1441 gctggaagta gccgccaagt cgctcggcca tgagccagga ggcctcggcg tggcggccct
     1501 cctcctgcgt ccgcatcgtg aagaagaagc gcaggtcggg cagccggttc tcgaggcaga
     1561 agcgcaggag cagggccggg ctctccgaga tggcgccgta ctcgccccag gtccgccggc
     1621 tccagtgaag ccgcgcggcc atgatctgtt cctcggggta gcgctccggg tgcagctcct
     1681 cccacgggat atccagctcc gggtcccact cgagcgtctt ggctcgctgc cacagctccc
     1741 agacatcggg catggcggag gcgagctcga gcggatagcg ggtctcgggg agataggtgc
     1801 tcatgacggc attctcctgt acggggcgat cgagcggcgc ctcatgcgcg gccgagctcg
     1861 ggatggtcga cgggggccag cgtgacgtcc cacgtggcga ggcgctcccg cacctgggcg
     1921 agggtggcgc gcaccaccgc cttctcctgg gccacggtgg aggcgcccag gccgtgctcc
     1981 gcggtgaggg cctccgcggc gagcaggggc tcgcggctct ccgcgggcag tagccacgtg
     2041 ttccggtagc cggtgaggat cacgcgctcc agcatatcgc gaaccgcgcc accca
//
LOCUS       DATMCV010000811         6720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_110550, whole genome shotgun sequence.
ACCESSION   DATMCV010000811 DATMCV010000000
VERSION     DATMCV010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110550"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VNU03_18565"
     CDS             <1..978
                     /locus_tag="VNU03_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76530.1"
                     /translation="AYVAHPVGAGPYKFVEYIPGSRLVLEANPTYWGAKPAFKRVTVR
                     IIPENATRVAALQTGEVLAIYNVPPDSIDRLKGNKDLVVQDTLTTRYNYLYLQNDRPP
                     FNDKRVRLAINYALDKDQITRVIFKGLAKPATAPMGPATAFFDKSLSPYPFDVAKAKA
                     LLTEAGYPNGLKFVMGTPFGRFINDRQVAEAVVGQLAKAGFTVDLRVEEWGTSLANLM
                     ARKYDAMFGAYGGSPDPDYMLSWLFTAKSSVVGFNKPEVEALLLEGLQAPDDAAARKT
                     YERIQKIVWEDVPLGFLYFQPDIVAYSKRLKGFGPRVDEYVDVRGARVE"
     gene            984..1904
                     /gene="nikB"
                     /locus_tag="VNU03_18570"
     CDS             984..1904
                     /gene="nikB"
                     /locus_tag="VNU03_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HWP76531.1"
                     /translation="MRNAYLRQIFQAAIVLLNVSLVVFVLVRIVPGDPARLMLGMEAS
                     EEAVQAARAQFGFDRPLYVQYVQFVGGALRGDLGQSVRFRRPVGDLLRESFPATLELA
                     LAATVIALLLAVPAGIYAAVHRDGVLDRLLMAGALIGQAMPIFWLGIMLITLFSVKLG
                     WLPTSGRGSLAQLIMPSVTLSTYVMALLARLTRSNMLDVLREQYVRTARAKGLRETVI
                     VHKHALMSAALPIVTVLGLQVGALLSGAVVTETVFNWPGIGTLALRAIQQRDYPVVQG
                     VVLVSAALFVLINGMVDLAYRYLDPRVRLT"
     gene            1921..2865
                     /locus_tag="VNU03_18575"
     CDS             1921..2865
                     /locus_tag="VNU03_18575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76532.1"
                     /translation="MSPATEIEVAGPFVLSEARTPRPSVFRHSGFLFGLVTVSVALLV
                     ALFPHAVATQDPLAQDIGNRLVPPAWMSGGSPQWLLGTDHLGRDILSRVLYGSRVSLA
                     VGLWSVLIGGPVGLLLGLVAGYRGGAVDSLVMRLVDLHLAFPFVLLAMAMIAILGPGL
                     WKMVAVFSITSWMLYARTVRSSVLALREREFVQGAWALGATGTRIVARHILPNVVNSL
                     VVLLSFDLARIILAESALSFLGLGVPPPTPSWGGMLSEGREYIEDAWWIAAFPGFAIM
                     LVTVGVSFLGDGLRDVLDPRFEPLNDRTRGGDVGTSKT"
     gene            2897..4951
                     /locus_tag="VNU03_18580"
     CDS             2897..4951
                     /locus_tag="VNU03_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP76533.1"
                     /translation="MDIGGTFTDVVLVRGTGQVKTAKVLTTPHEPERAVLEGIAKVLA
                     EEAVTAGRVARLIHATTLATNAIVERKGCRTAMLTTTGFRDVLETRNELRYDLYDLFL
                     DLPPAVVPRFLRIPVDERIRYDGKVLRAPEPVELEQTVQRLVAEQVDSVAVCFIHAYA
                     NPENEQRVGEMLRKHLPGLAVSLSSEVLSEIGEYGRFSTTAINAYVQPLVARYLERLS
                     GTLEADGFKGAFTVMASSGGTMPLATAKRFPVRLVESGPAAGVHAASAAGVALQIPRL
                     LSLDIGGTTAKMCVIQDGKANWTTDFEVARLSRFKKGSGLTLKVPSVDLIEIGAGGGS
                     IARVNALGLIEVGPDSSGSDPGPACYGLGGDFATVTDADVLLGYLNPANFLGGEMRLD
                     SAAAERAVMKAIGAPLRMDVRRAAYGINSIVNENMANAARVYTAERGIDIRSFTLFAF
                     GGAGPVHACGVAERLGISVVIVPPAGGVLSAIGCVLAPMAFDMVRGFKCDLDGADFAR
                     ANGLLAGMVEEGRSLLQQAGFTRGVVVEASADMRFAGQRSEVNVPLRRLTLPASSARI
                     IDRDFRAVYRARYGRDVPEVKPEFVNLRVTVRGPGHRGRLRPPVPRREARVSGPSSRR
                     PMFFEPRRGFVDCPAYERAALVKGQRITGPAVVEDRDSTAVVPPRWTATTDHVGNLVL
                     TR"
     gene            4960..6564
                     /locus_tag="VNU03_18585"
     CDS             4960..6564
                     /locus_tag="VNU03_18585"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP76534.1"
                     /translation="MRKIGTDPLLISMQWAKLQSVVDEASVTLLRTAFSRIVTEANDY
                     TCALFDTRGDMIIQSTQGLTSFTGVLAVAIKHFLAAFPVAELEPGDCLIMNEPWLGAS
                     QVNDMHVVTPIFHRRRVVAYAANISHCPDVGGRILSGDSREVFEEGIRLPMSKLFKRG
                     KPNDELLRIFLNNVRVPDIVRGDLMAQWSSNVIMERGVLAFLREYRLDSLTGIAREIL
                     SRSEAAMRAAIREIPDGTYASEIETDGFDEPLRICTSVTVRGDRISVDYAGTSPQTPV
                     GINCCWNYTYAETVFPLICVVRPSTPVNGGCLRPLTVVAPEGSVLNPRFPAAVGARAM
                     ASMFLPAAVFRALASAVPDRVLADPSAPTWIPTCSGINQYGKRFADIIFMNGGLGARP
                     TKDGINVIGFPANISSTPVEIFENEKPMMVTEKQLISSSGGSGRYRGGCGQRCSITSY
                     SEAPITFAMRGDRVRHPALGLFGGGAGAPGAVTLNGQPIHSKRTIQLQAGDRLVLETP
                     GGGGYGDPAERLEAARDEDRKNGYTG"
BASE COUNT          987 a         2281 c         2315 g         1137 t
ORIGIN      
        1 gcctacgtcg cccatccggt cggcgcgggc ccctacaagt tcgtcgaata catcccggga
       61 tcgcggctcg ttctcgaagc gaatcccacc tactggggag ccaagccggc cttcaagcgg
      121 gtcacggtcc ggatcatccc cgagaacgcc acccgggtcg ccgcgctgca gaccggagaa
      181 gtgctcgcca tctacaacgt tccccccgac tcgatcgatc gtctgaaggg gaacaaggac
      241 ctggtggtcc aggacacgct caccacccga tacaactatc tctatctcca gaacgaccgg
      301 ccgccgttca acgacaagcg ggtgcgcctg gcgatcaact atgccctcga caaggatcag
      361 atcacgcgcg tcatcttcaa gggcctcgcc aagcccgcca cggccccgat ggggccggcc
      421 accgcgttct tcgacaagag cctgagccca tatccattcg acgtggccaa ggccaaggcc
      481 ttgctgaccg aggccggata ccccaacggg ctcaagttcg tcatgggcac gcccttcggc
      541 cgcttcatca acgatcggca ggtcgccgag gcggtggtgg gacagctcgc caaggccggc
      601 ttcacggtgg acctccgggt ggaggagtgg gggacgagcc tcgccaacct catggcccgc
      661 aagtacgacg cgatgttcgg cgcctacggg ggctcgcccg accccgacta catgctgagc
      721 tggctcttca cggccaagag cagcgtggtg ggcttcaaca agcccgaggt cgaggcgctg
      781 ctgctcgagg gcctgcaggc ccccgacgac gcggcggccc gcaagaccta cgagcggatc
      841 cagaagatcg tgtgggagga cgtgccgctc gggttcctct acttccagcc cgacatcgtc
      901 gcctacagca agcggctcaa gggcttcggc ccccgcgtcg acgagtacgt ggacgtgaga
      961 ggcgcgcggg tcgaatagac gggatgcgga acgcctatct ccggcagatt ttccaggcgg
     1021 ccatcgtcct gctcaacgtg tcgctcgtgg tcttcgtcct ggtgcggatc gtgccgggcg
     1081 atcccgcgcg tctcatgctg gggatggagg cgtcggagga ggcggtccag gccgcccgcg
     1141 cccagttcgg cttcgaccgg cccctctacg tccagtacgt ccagttcgtc gggggcgcgc
     1201 tccggggcga cctggggcag tcggtccgat ttcgacggcc ggtaggggac ctcctgcgcg
     1261 agagctttcc cgccaccctc gagctggccc tggccgcgac cgtgatcgcg ctgctcctgg
     1321 ccgtgcccgc cggcatctac gccgccgtgc accgcgacgg ggtgctggat cggctcctca
     1381 tggcgggggc gctgatcggg caggcgatgc caatcttctg gctcggcatc atgctgatca
     1441 cgctgttctc ggtgaagctc ggctggctgc ccacctcggg ccggggctcg ctcgcgcagc
     1501 tgatcatgcc gagcgtgacc ctctccacgt acgtcatggc cctgctggcc cggctgacgc
     1561 gctccaacat gctcgacgtc ctgcgcgaac agtacgtgcg caccgcgcgg gccaagggcc
     1621 tgcgggagac cgtcatcgtg cacaagcacg ccttgatgag cgccgcgctg cccatcgtca
     1681 cggtgctggg gctgcaggtc ggcgccctgc tgagcggcgc ggtcgtgacc gagaccgtct
     1741 tcaactggcc ggggatcggc accctggccc tccgggcgat ccagcagcgc gactacccgg
     1801 tggtgcaggg ggtcgtcctg gtcagcgccg cgctcttcgt gctgatcaac gggatggtcg
     1861 acctggccta ccggtatctc gatccgcgcg tccggctcac gtgagatcgg ccggggcgcg
     1921 gtgagtccgg ccaccgagat cgaggtcgcg ggtccgttcg tcctgtcgga agcgagaacg
     1981 ccgcgcccgt ccgtcttccg ccattccggg ttcctcttcg gcctggtcac cgtgtcggtc
     2041 gctctgctgg tcgccctctt tccgcacgcg gtggcgaccc aggatccgct cgcccaggac
     2101 atcggcaatc ggctcgtccc cccggcctgg atgtccgggg gcagcccgca gtggctgctg
     2161 ggaaccgatc acctcggccg agacatcctg agccgggtgc tctacggcag ccgggtatcg
     2221 ctcgcggtgg gcctgtggtc ggtcctcatc ggcggcccgg tgggactgct cctcggcctg
     2281 gtggccggtt accggggcgg ggccgtcgac agcctggtca tgcgcctcgt ggacctccac
     2341 ctcgcctttc ccttcgtcct gctcgcgatg gccatgatcg cgatcctggg ccccgggttg
     2401 tggaagatgg tggcggtgtt ctcgatcacg agctggatgc tgtacgcgcg taccgtccgc
     2461 tcctcggtgc tggccctccg ggagcgggaa ttcgtccagg gcgcctgggc cctcggggcg
     2521 accgggaccc ggatcgtcgc ccgccacatc ctgccgaacg tggtgaattc cctggtcgtg
     2581 ctcctgtcct tcgatctggc ccggatcatc ctcgccgagt cggccctgag cttcctcggg
     2641 ctcggcgttc cgccgcccac gccgtcgtgg gggggaatgc tcagcgaggg ccgcgaatac
     2701 atcgaggacg cgtggtggat cgcggccttt cccggattcg cgatcatgct cgtcacggtc
     2761 ggcgtgagct tcctgggaga cggcctgaga gacgtcctcg atccccggtt cgaacccctc
     2821 aacgatagga cccgcggagg cgacgttggc acatccaaaa cgtagcgcga catcaggcga
     2881 tcggctcgag ctcggcgtgg acatcggcgg caccttcacc gacgtggtcc tcgtgcgcgg
     2941 cacggggcag gtcaagacgg ccaaggtgct gaccacgccg cacgagcccg agcgggcggt
     3001 gctggaaggg atcgccaagg tgctcgcgga ggaggcggtc accgcgggcc gggtcgcgcg
     3061 gttgatccac gccaccacgc tggcgacgaa cgccatcgtc gagcgaaagg gctgccggac
     3121 cgcgatgctg accaccaccg gctttcgcga cgtcctggag acgcggaacg agctccgtta
     3181 cgacctctac gatctcttcc tcgatctgcc gcccgcggtg gtcccgcgct tcctgcgaat
     3241 tccggtcgac gagcgcatcc gctacgacgg caaggtgctg cgggctccgg agccggtgga
     3301 gctcgagcag accgtccagc gcctggtggc cgagcaggtc gactcggtcg cggtgtgctt
     3361 cattcacgcg tacgccaacc ccgagaacga gcaacgagtg ggggagatgc tgcggaagca
     3421 tctcccgggg ctggcggtgt ccctctcgtc cgaggtgctg tccgagatcg gcgagtacgg
     3481 ccgcttttcc accaccgcga tcaatgccta cgtgcagccg ctggtggccc gctatctcga
     3541 gcgcctatcc ggcaccctgg aggcggacgg gttcaagggc gcattcacgg tgatggcatc
     3601 gagcggcggc acgatgcccc tggccaccgc caagcggttc ccggtccggc tcgtcgagtc
     3661 cgggcccgcg gccggggtgc acgccgcctc ggcggccgga gtggcgcttc agatcccgcg
     3721 cctcctctcg ctcgacatcg ggggcaccac cgccaagatg tgcgtgatcc aggacggcaa
     3781 ggcgaactgg acgaccgatt tcgaggtcgc gcggctcagc cgcttcaaga aagggagcgg
     3841 gctgaccctc aaggtcccga gcgtggacct gatcgagatc ggggccggag gcggcagcat
     3901 cgcgcgcgtc aacgcgctcg gcttgatcga ggtggggccg gatagctccg gatccgaccc
     3961 cgggccggcc tgctatggcc tgggcggaga cttcgccacc gtcacggacg cggacgtgct
     4021 cctgggctat ctcaatccgg ccaatttcct cggcggggag atgcggctgg attcggccgc
     4081 cgccgagcgg gcggtcatga aggcgatcgg ggctccgctc cggatggacg tccggcgcgc
     4141 ggcctacggc atcaactcca tcgtgaacga gaacatggcc aacgcggctc gggtctatac
     4201 cgcggagcga ggtatcgaca tccggtcgtt caccctcttt gccttcggag gcgccggccc
     4261 ggttcatgcg tgcggggtgg ccgagcgtct cggcatctcg gtggtgatcg tgcccccggc
     4321 cggcggggtc ctgtccgcca tcggctgcgt cttggcgccg atggccttcg acatggtgcg
     4381 cgggttcaag tgcgacctcg acggggccga cttcgcgcgg gccaacggac tcctcgcggg
     4441 catggtcgag gaggggcgat ccttgctcca gcaggccggg ttcacccggg gcgtggtggt
     4501 ggaggcttcg gccgacatgc gcttcgccgg gcagcgctcg gaggtgaacg tgcccctgcg
     4561 gcgcctcacg ctgccggctt cgtcggcgcg aatcatcgac cgggacttcc gggccgttta
     4621 tcgggctcgc tacgggcgcg atgtcccgga ggtcaagccg gagttcgtga atcttcgggt
     4681 caccgtgcgc ggcccgggcc accgcggacg gctccggccg ccggtaccgc gtcgggaggc
     4741 gcgcgtgtcg ggtccctcgt cccgacggcc catgttcttc gagccgaggc gcggcttcgt
     4801 cgactgcccg gcctacgagc gcgccgccct cgtgaagggg cagcggatca ccggtccggc
     4861 ggtcgtcgaa gatcgggact cgaccgcggt cgtgcctccc cgctggacgg ccacgaccga
     4921 tcacgttggc aatctcgtcc tgacgcggtg aggagcccca tgcgaaagat tggaaccgat
     4981 ccgttgctga tcagcatgca gtgggcaaag ctgcagtcgg tggtcgacga ggcgagcgtg
     5041 accttgctcc ggaccgcctt ctcgcgaatc gtgaccgagg ccaacgacta cacgtgcgcg
     5101 ctcttcgata cccggggcga catgatcatc cagtccaccc aggggctgac gtcgttcacc
     5161 ggcgtcctcg ccgtcgcgat caagcatttt ctcgccgcct tcccggtggc ggagctcgag
     5221 ccgggcgact gcctgatcat gaacgagccg tggctgggcg ccagccaggt caacgacatg
     5281 cacgtggtca cccccatctt ccatcggcga cgggtggtcg cgtatgccgc caacatctcg
     5341 cactgtccgg acgtgggagg acggatcctg tccggggatt cacgcgaggt attcgaggag
     5401 ggcattcgcc tgccgatgtc caagctgttc aagcgcggca agccgaacga cgagctcctc
     5461 cggatcttcc tgaacaacgt ccgggtgccg gatatcgtcc ggggcgatct gatggctcag
     5521 tggtcgtcga acgtgatcat ggagcgcggg gtcctggcgt tcctccgcga gtatcggctg
     5581 gactcgctca ccgggatcgc gcgggagatc ctgagccggt cggaggcggc catgcgcgcg
     5641 gccattcgcg agatcccgga cgggacctac gcgagcgaga tcgagacgga tggcttcgac
     5701 gagccccttc ggatctgcac gtcggtcacg gtgcggggag accggatcag cgtggactat
     5761 gccgggacgt cgccccagac gccggtcggc atcaattgtt gctggaatta cacgtacgcg
     5821 gaaacggtct tcccgttgat ctgcgtggtc cggccgtcca cgccggtcaa cggcgggtgc
     5881 ctgcgtccgc tcaccgtggt ggcacccgaa ggaagcgtct tgaaccctcg gtttcccgcc
     5941 gcggtgggtg ctcgggccat ggcgtcgatg ttcctgcccg ccgccgtctt ccgggcgctc
     6001 gcgtcggcgg tgccggaccg ggtgctcgcc gatccgtcgg cgccgacctg gatcccgacc
     6061 tgctccggca tcaatcaata cgggaagcgt ttcgcggaca tcatcttcat gaatggaggc
     6121 ctcggcgcgc gccccacgaa ggacggcatc aacgtcatcg gcttcccggc caatatctcg
     6181 agcacccccg tcgagatctt cgagaacgag aaaccgatga tggtgaccga gaaacagctg
     6241 atctcgagct ccggcggctc cggccggtat cggggagggt gcgggcagcg ctgttcgatc
     6301 accagctact ccgaggcccc gatcacgttc gcgatgcgtg gcgaccgggt ccggcatccc
     6361 gcgcttggac tcttcggagg cggcgcgggt gctccgggcg ccgtcaccct caatggccag
     6421 ccgatccaca gcaagcggac catccagctc caggcggggg accggttggt cctggagacc
     6481 ccgggcggcg gtggctacgg cgatccggcg gagcgtctgg aggcggccag agacgaggat
     6541 cggaagaacg gatacacggg gtaggggctt tcgggacccc tcacccctgc cctctccccg
     6601 gaggggagag ggtttcgatc aggggagggt tgcggtcagg cgcgggcgtc ggagggggtg
     6661 ttctgctcca cccactcgag cgcgcggctc acctcgaagc cttctgcctc cgcctcggtg
//
LOCUS       DATMCV010000812         3952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_242659, whole genome shotgun sequence.
ACCESSION   DATMCV010000812 DATMCV010000000
VERSION     DATMCV010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242659"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VNU03_18590"
     CDS             complement(<1..411)
                     /locus_tag="VNU03_18590"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76535.1"
                     /translation="MTRILAIAALLLGAVPSAALANEGGGGLISLDKSIVIQMINFGL
                     LLFILIKLLYKPLLAKMEERTQAIRKSLEEAQAARAEAERERSEHAAKLQAAYAEAQA
                     IRAAALKEAGEEQHRLLEAARGEATRLVESARSEL"
     gene            complement(490..1602)
                     /gene="mnmA"
                     /locus_tag="VNU03_18595"
     CDS             complement(490..1602)
                     /gene="mnmA"
                     /locus_tag="VNU03_18595"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWP76536.1"
                     /translation="MKERIVVGMSGGVDSSVAAALLVEQGVEVIGVTLRVWPWREPEE
                     STRKFGSCCSPETVADARAVARRLGIAYYLLNTEREFERAVIEPFARAYAEARTPVPC
                     VACNREVKFGSLVQRARAWDAVAVATGHYARITRESSGGRFLLWRGRDARKDQSDFLW
                     PLTQSQLEAARFPVGDLTKEAVREKARALGLETADKPESQEICFIPDDDYRGFLRRRM
                     PEAFAPGPIVDGQGTVLGRHEGLVNYTVGQRRGLGLSSRRPLYVTALDPGRNALVVGE
                     AAAVEVDRLQAEQVNLISVPALTGPLAVAAKIRHSHEPAAATIEPGEPGEPDGEGTVL
                     VRFERAQRAPAPGQSVVFYQGDLVVGGGVIARPKGA"
     gene            complement(1613..2473)
                     /locus_tag="VNU03_18600"
     CDS             complement(1613..2473)
                     /locus_tag="VNU03_18600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWP76537.1"
                     /translation="MVNRQHGLGRGLGALLSSPGPSSTEAPGTTTVELPIDSISPNPQ
                     QPRKDFDDKSLHDLSASLKQSGVLQPVVVRRLGDGYQLVVGERRWRAAKLAGITRIPA
                     VVREASDAESLELALVENLLREDLNPMEEAEAYQRLLAEFAWTQEELAQRVARDRSSI
                     ANCLRLLKLPDVIQADLRGGRLTMGHARALLSLDSPAEQLRLREEILTHSWSVRTTEQ
                     GVQAKRTQPTRRLLRRSAELAAVEDALRVALATRVRIVGSERAGRIEVSYSSREELDR
                     LTELISTTSR"
     gene            complement(2466..3227)
                     /locus_tag="VNU03_18605"
     CDS             complement(2466..3227)
                     /locus_tag="VNU03_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP76538.1"
                     /translation="MGRIVAVVNQKGGVGKTTTAVNLATGLALAERRTLLVDLDPQGN
                     ATTGLGVDKAGLERTIYHVVLEGATIADVAIPSLLPFLHLVPSDIDLVGAEIELVNQD
                     HREGRLKTALIAVGPDYDYIVIDCPPSLGLLTLNALAAADSALVPLQCEYYAMEGLAH
                     IIRTIGLIRERMNPQLKVEGVVLTMYDGRTSLAAQVRAEAQQHLGKQVMTAVIPRNIR
                     LSEAPSHGKPIMLYDLRSAGSTAYLDLAREVISHG"
     gene            complement(3272..>3952)
                     /gene="rsmG"
                     /locus_tag="VNU03_18610"
     CDS             complement(3272..>3952)
                     /gene="rsmG"
                     /locus_tag="VNU03_18610"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HWP76539.1"
                     /translation="AWGKLAGVPGTPPDIFRELLAAIRPLAGRPATAEHREQFQKYLD
                     VFLRWNRVHRMTALGSPAEIVRGLFLDSLLFFSVLPPRPLRVVDIGAGSGVPGLPLRL
                     VDPGISLTLIEAKRKRVSFLLAACRELSLPDVSVVEGRAEDLMAADSALAGGFDAVVA
                     RAVGSEKRLFPISLRYLRPGGAVVLAGSPASTGQGGMQVVKVSVPGGRSTRVFLRAAK
                     ESSVPRGT"
BASE COUNT          656 a         1372 c         1314 g          610 t
ORIGIN      
        1 cagctcgctg cgggccgact ccaccaggcg cgtggcctcc ccgcgggccg cttccagcaa
       61 ccggtgctgc tcctcgcccg cctccttgag tgcggcggcc cggatcgcct gcgcctccgc
      121 gtacgcggcc tgcagcttcg cggcgtgctc cgagcgctcg cgctccgcct ccgcccgcgc
      181 ggcctgcgcc tcctcgagcg acttgcggat cgcctgggtg cgctcctcca tcttggccag
      241 cagcggcttg tacagcagct tgatcaggat gaagagcagc aggccgaagt tgatcatctg
      301 gatgacgatc gacttgtcca ggctgatcag gccgcctccg ccctcgttgg ccagcgccgc
      361 cgacggcacc gccccgagca gaagcgcagc gatggcgagt atgcgagtca tggtgagttc
      421 tccggcccga gcgctattgt gaaagaagga acaaagggta tccgagtatc tccgtgaggg
      481 ccgggactgt caagcgcctt tgggccgggc gatgaccccc ccaccgacca ccagatcgcc
      541 ctgatagaag accaccgact ggccgggagc cggggcccgc tgggcccgct cgaaccggac
      601 caggacggtc ccctccccgt cgggctcgcc gggttcgccg ggttcaatcg tcgcggcggc
      661 cggctcgtgg ctgtgccgga tcttggccgc caccgccagc gggccggtca gggccggcac
      721 cgagatcaga ttgacctgct cggcctgcag ccggtccacc tcgaccgcgg cggcctcccc
      781 gaccacgagc gcgttccggc cgggatcgag cgcggtgacg tagagcggtc ggcgcgagga
      841 aaggccgagg ccgcgacgct ggcccaccgt gtagttgacc aggccttcgt gccgcccgag
      901 cacggtgccc tgcccgtcga cgatcggccc gggggcgaag gcctccggca tccgccggcg
      961 caggaagccg cggtagtcgt cgtcggggat gaagcagatc tcctggctct ccggcttgtc
     1021 cgcggtctcg aggccgaggg cgcgggcctt ctcgcgcacc gcctccttgg tgagatcgcc
     1081 caccgggaag cgcgccgcct cgagctgcga ctgcgtgagc ggccagagaa aatccgactg
     1141 atccttgcgc gcgtcgcggc cgcgccagag caggaagcgc ccgccggacg actcccgggt
     1201 gatgcgcgcg tagtggccgg tggccaccgc gaccgcgtcc cacgcacgcg cgcgctgcac
     1261 cagcgagccg aacttcacct cgcggttgca cgccacgcac ggcaccggag tgcgcgcctc
     1321 cgcgtaggcg cgggcgaacg gctcgatcac cgcgcgctcg aactcgcgct cggtgttgag
     1381 caggtagtac gcgatgccga gacgccgcgc gaccgcacgc gcgtccgcga cggtctcggg
     1441 cgagcagcag ctcccgaact tccgcgtgga ttcttcgggc tcgcgccacg gccacacccg
     1501 cagcgtgact ccgatcacct cgacgccttg ctcgacgaga agcgcggcgg cgaccgacga
     1561 gtcgacgccg ccgctcatgc cgacaacgat gcgctccttc atgcgctcga gtctagcgcg
     1621 acgtcgtcga gatcagctcg gtcagccgat ccagctcttc gcgagaggag tagctgacct
     1681 cgatgcgccc ggcgcgctcg ctgccgacga tgcgcacccg cgtggcgagc gccacgcgca
     1741 gcgcgtcctc gaccgcggcc agctccgccg agcggcgaag cagccggcgc gtcggctgcg
     1801 tgcgcttggc ctggacgcct tgctccgtcg tgcgcaccga ccacgaatgg gtgaggatct
     1861 cttcccggag tcggagctgc tcggcagggg agtcgagcga gagcagagcg cgcgcgtggc
     1921 ccatcgtgag ccgaccgcca cggagatcgg cctggatcac gtccggcagc ttcaacaggc
     1981 ggaggcaatt cgcgatgctg ctccgatcgc gcgccactcg ctgcgccagc tcttcctgcg
     2041 tccaggcgaa ctcggcgagc agtcgctgat acgcctcggc ttcctccatc gggttcaggt
     2101 cctcgcgcag caggttctcg acgagcgcga gctccaggct ctcggcatcg ctcgcttcgc
     2161 ggacgaccgc cggaatgcgg gtgatcccgg ccagcttcgc cgcgcgccac cgacgctcgc
     2221 cgacgacgag ctggtaaccg tcaccgaggc ggcggacgac caccggctgc agcacgccgc
     2281 tctgcttcag tgaggccgaa agatcatgta aagacttatc atcaaagtct tttcttggct
     2341 gctgtgggtt gggggagatg gagtcgatcg ggagctcgac ggtggtggtc cctggcgcct
     2401 ccgtggacga agggccgggg gaggagagca gggcccccag gccgcgccca agtccgtgct
     2461 gtcgattaac catgtgagat cacctctctg gcaaggtcga ggtaggcggt ggagcccgcg
     2521 gaccgcagat cgtagagcat gatgggctta ccgtggctag gcgcttcact aagccttata
     2581 tttcgaggta ttacagccgt cattacctgc ttgccgaggt gctgttgtgc ctcggcccgg
     2641 acctgcgcag cgaggctggt gcggccatcg tacatggtga ggacaacacc ctccaccttg
     2701 agctgagggt tcatccgctc gcggataagc ccgatcgtcc tgatgatatg ggccagccct
     2761 tccatcgcgt agtactcaca ctgcagcggt acgagcgcgg agtcggcggc agccagagca
     2821 ttcagggtca gcaggccaag ggaagggggg caatcgatga cgatgtagtc atagtcgggc
     2881 ccgaccgcga tgagggccgt cttcaaccgt ccctcgcggt gatcctgatt aaccagctcg
     2941 atctcggctc ctaccaggtc aatgtccgat ggaacgaggt gtaggaaggg gagaagggac
     3001 gggatcgcca cgtccgcaat tgtcgcgccc tcgagaacga catgatagat cgttcgctcg
     3061 agtcccgcct tgtccacccc taggccggtg gtcgcgttgc cctgagggtc caagtccacg
     3121 agaagcgtcc gccgctcggc gagggctagg ccggtcgcga gattgacggc ggtcgtcgtc
     3181 ttgccgaccc cgcccttttg gttcaccacc gccacaatcc gccccacttg ctagggaccc
     3241 tttctcaatg ttccacgtgg aacgctgcca ctcatgttcc acgtggaaca ctactctctt
     3301 tcgccgcccg gagaaagact ctggtgcttc ggccccccgg aacggagacc ttgaccacct
     3361 gcataccgcc ctgcccagtc gaggccggag agcccgccaa aaccacggct cctcctggcc
     3421 ggaggtagcg taaggaaata gggaacaacc gcttctcgga gccgactgcc ctcgccacaa
     3481 ccgcgtcgaa ccctccggcg agggccgagt cggccgccat caggtcctcc gctcggcctt
     3541 ccaccaccga gacgtcaggc aggctcagct cccggcaggc ggcgagcaga aaggacaccc
     3601 gcttccgctt cgcctcgatc agggtcaggg agatgcccgg gtcaacgagg cgaagcggaa
     3661 ggcccgggac accactgccc gccccgatgt cgacaaccct gagcgggcga ggcggtagca
     3721 cggaaaagaa caagagagag tccaggaaga ggcctcgaac gatctcggcg ggggagccga
     3781 gcgcagtcat gcggtgcacc cgattccacc gcaaaaagac atcgaggtac ttctgaaact
     3841 gctcccggtg ctcggccgtg gcgggccgcc cggccagcgg gcggatcgcc gcaagcagct
     3901 cacggaagat gtcaggaggg gtgccgggca cgccagcaag cttaccccag gc
//
LOCUS       DATMCV010000813        13034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_643054, whole genome shotgun sequence.
ACCESSION   DATMCV010000813 DATMCV010000000
VERSION     DATMCV010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643054"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VNU03_18615"
     CDS             <1..641
                     /locus_tag="VNU03_18615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76540.1"
                     /translation="RESIVKELWRGRGVVPNGPIAKGDNHFDASLPPLAYNPAEAKAR
                     LQKAGYKGEEIFIETTVAYVAQDKAMAEAIAAMWKDVGVNVKVEVIEYSVRAQKNREK
                     SFKGVFWSDPTSTLSDPDGMMWRLLGPGGPQDYWREAKFDELGNAARFSVDEKFRGTA
                     YKDMTKIFLEHLPWLPVIQPYEDYGIQKYIDFTPNPNQQFEIRRFNFKFRRA"
     gene            709..1635
                     /locus_tag="VNU03_18620"
     CDS             709..1635
                     /locus_tag="VNU03_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76541.1"
                     /translation="MRGFVPFLGQRLFRALIALWLVSTVVFVVMRLSGDPVPLLLPPD
                     APTSEILRVRRELGLDKPLPAQYVIFLGNLARGDFGRSIHFREPAFNVVRGYLAATLE
                     LGLTSFVLAALIAVPIGLFSAMRRNTLVDHVAMGLALIGQSAPTFFLGILFILLLSLK
                     ADLFPTSGRGDWRNLVLPALTLGAFTMASIARLTRSAVLEVLGADYIRTARAKGVAEA
                     WVVAKHTLKNAAIPIVTITGLQFGTLLGGAVVTETVFSWPGIGRLAVQSIYNRDYAVV
                     QCTVFLAALMFVVINFFVDLIYGVLDPRIRAG"
     gene            1658..2509
                     /locus_tag="VNU03_18625"
     CDS             1658..2509
                     /locus_tag="VNU03_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012765412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76542.1"
                     /translation="MRPKARRRRTPVLVLLGVGFVAVLLLVAAAAPLLAPQDPIRQSL
                     RGRLAAPTWNGSDGRAHLLGTDHLGRDVLSRVIFGSRVSLVIGFSAVLVGGVLGSAAG
                     LAAGFSRGRMDAIIMTVADAQLAFPFILLAIGIIAVLGPSFPTLVVVIGLSGWVSYAR
                     ILRSQVLVLRSREFVESIHALGGSLARVVLRHVLPNVLSSIVVIATLELARAIVLEAT
                     LSFLGLGIQPPTPSWGGMVQEGREYLDSAWWISTCPGIVLMVTSIVVSRTGDWLRDVL
                     DPTLRGE"
     gene            2529..2930
                     /locus_tag="VNU03_18630"
     CDS             2529..2930
                     /locus_tag="VNU03_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP76543.1"
                     /translation="MPVTALIEYGAASPEVRAVYDDIMATRKTDWINNFWKALANHPP
                     TLRRTWESIKQIMGAGALDPLTKEMVYLAVSAANGCDYCIASHTAGARRAGMSETMRG
                     ELLAVVGMACETNALANAFQVPVDERFRDPA"
     gene            complement(2891..3838)
                     /locus_tag="VNU03_18635"
     CDS             complement(2891..3838)
                     /locus_tag="VNU03_18635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWP76544.1"
                     /translation="MSRAAIADGVAFRRRGRLLVAAAAVSWSLGGLMARNVGTDPWTT
                     VFWRGLFCAAFLVAVTAVREGRRTPGVFAGMGGTGVGMAACFAIGSTCFIMALRHTSV
                     ANVLIIQSLSPFVAGLLGWIWMGERVAGRTWLAMGVALLGTAVMVSRYFYSTPASESL
                     GGDLLAFAVALTFALATVLLRRRRQVQMLPAAALAAALTSAIAWGSATPRAAGAGDLA
                     LLALFGAGQLGLGMILFTAGARRIPVAEASLIAVLESVLGPVWVWLALGENPGAPSLV
                     GGAVVLAALAGHTLADLRLEREIPADPQAGSRNRSSTGT"
     gene            complement(3835..4701)
                     /locus_tag="VNU03_18640"
     CDS             complement(3835..4701)
                     /locus_tag="VNU03_18640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWP76545.1"
                     /translation="MTVAALLLVAGAAVLHAGWNALAKRAGEPVLFLWWVGVVASGLY
                     APIALAVLGREGFRAAAIPFVIGTVLLHALYFFSLGRAYAAGDYSLVYPIARGLGVAL
                     VPMAAFALFDERLSPLGTLGVILVAAGIFFVHWGLRGRPGAESVAPRHRSGTGWALAT
                     GVLIAAYSLLDRAGVSRMSPLAYIWLMELGSCVLLAPVALARRDALGGEWRRNRATIV
                     AAALMSPTAYLLVLFAFQLSKTGYVVAAREMSIVLSAIIGAAWMKEDTGMRRLAGAAV
                     VLAGVACVALAR"
     gene            4761..5381
                     /locus_tag="VNU03_18645"
     CDS             4761..5381
                     /locus_tag="VNU03_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP76546.1"
                     /translation="MAYSLRLIEDRLAANAAAPVSRASRVLYAVEGALLADGISEPAP
                     AVGPGAARARAATTAARVYRVELVRQPPPSAGGRVLLEHPIDLAGGSGWLMRCDRVDF
                     DPGGVAPRHRHQGGGIRCLLQGRLEVTVGESAPRAVVPGEAWFESGREPVLAVAARDA
                     ETSFIRVSILPAAIAGRSSILYVDPAAAAGGRPRRYTVYVDEPIAL"
     gene            5384..6505
                     /locus_tag="VNU03_18650"
     CDS             5384..6505
                     /locus_tag="VNU03_18650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP76547.1"
                     /translation="MRVLILTASYGSGHAEAARSLAGAFASRGAEPVVVDHFRELVHP
                     VFARLSGATYYWLLRHAPSLWALGYSLGDRLGPESPLAFGVPSLGTASLGRLLDAMQP
                     DAVVTVHATPAAAMAALAASGRALPPHTTAVTDFVAHSQWMPRPVDRYCVAAEEVRNE
                     FVARGIPPDRVIVTGVPVREEFSRPVEGAAARRAWDLAPRLPTVVAMAGTQGSLGRLY
                     DVVLALLTVERPLQAVVVAGRDERLRARLARLAAGSRVRVVGYVEDVRTLLAAADLLV
                     TKAGGMTLAEATATEVPLLLYGSLPGQEIGNERFASRAGIALVARSRGELRRLLDRAL
                     ADPETVERMQGSLRRLRRPDAAAHIVDLVLEQIATRRRG"
     gene            6506..8299
                     /locus_tag="VNU03_18655"
     CDS             6506..8299
                     /locus_tag="VNU03_18655"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP76548.1"
                     /translation="MSQFAFCLESGDRALREGRYADAEQALAAALALDPRSVKAQFKL
                     GLTLERQRKWAPAETAFRAAIRGKRDHATAWLRLSAVCRRLGKAREEETAAREAVRLA
                     PRSSEAHHGLAVTLARLGRAEEAVAAFEETLRLAPSSAVAHGGLGAALTTLGRHGDAA
                     AAFGRAARLDPASYKAHLQHAEAHRRQRRWGEAVDAYRAAAALRPAVPALHGRLGLML
                     ERQRRYREAVAAYTRAARLDTAGARWRARLGGVLERQGRYGEAEAALLEALRREPDRL
                     DAHLSLAMIYRRQGKWGDEARVYERAIGLKPGDAHLHADLGLALEAQGLLGPAEGQFR
                     RALALAPENADLHVELGIAMGRQGRHAEAEAALREAVRLNPQDLVARANLALGLGRQG
                     RHAESEAAYRQALSLKADAAPLHRGLGMALYSQAKRTEAEAAFREAVRLKPSYARAWG
                     DLGELLTETGRHDEAVEAFREAIAVKPGRIASHRGLGTARLLQERPDLAETAFRGALA
                     LRPDDSQSHFGLWTALERQGKLVEAEAAYRDAIAMSADLAEAHSRLGELLARQGRWRE
                     AETAYREALRRSPDDARSRVALTAVLRRARG"
     gene            complement(8296..8949)
                     /locus_tag="VNU03_18660"
     CDS             complement(8296..8949)
                     /locus_tag="VNU03_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76549.1"
                     /translation="MSRGRTYTLRNAASIVFALTAVIPLLLFTYTLYALNQLKLTEAQ
                     MGLGVALACALVGLFIFSNLMARLSEVLRYLEEQQDAGGRAGAGAAASAVPGVGGDMV
                     ARPVAAATGSNPAAAVFPGGLQVPGLGTITAASRAASAARQAAGIFDEVQKTMWQAEA
                     QRHLGRAVLISVKNSTEPIKGSLAQIADDGVLLEQGDERVAVSYLRITNIEVDPTDT"
     gene            9059..9850
                     /locus_tag="VNU03_18665"
     CDS             9059..9850
                     /locus_tag="VNU03_18665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HWP76550.1"
                     /translation="MSPSRRTLRDMRGFVFDLDGCVWTGDVLTPGAAQVLALIRQQGK
                     ALCFLTNNSRARARTMQAKLVRLGVEAKEHEVLTPLEILGEVVTERWGPSSVLAIGGP
                     ELAEVLVEAGHRVVPIDRSRDATVVVVGNDFDFSYERLTAAARAVAGGAAFLTPNIDP
                     RLPMENGEFLPGCGAIVEAVATAAGLRPIVVGKPEPPLFELALKRMGLGVDEAAMVGD
                     SVDSDVRGARRVGMTAVLFAPKGGLDGVAHYLVKSMSQLRRLLGG"
     gene            9853..10548
                     /gene="pepE"
                     /locus_tag="VNU03_18670"
     CDS             9853..10548
                     /gene="pepE"
                     /locus_tag="VNU03_18670"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HWP76551.1"
                     /translation="MRVLLHSGGPLGPLGRAALPDFLGPLRRVAFVTAASLHDETASF
                     ERVRAALGAPPPDGAGLELLHLRWNHDPLETLAGAEALFMGGGNTYALLQRLRGAGLL
                     EAIRARVAAGMPYMGASAGSNVAGPTILTTNDWNVVALDRFDALGLVGFNINPHYKEA
                     DPAMAPYSETRDDRIREYHVVNANPVLGLEEGAWVIVEDGVVTAHGTARVKLFRPAAD
                     PVWYRAGDRLPVT"
     gene            complement(10643..11344)
                     /locus_tag="VNU03_18675"
     CDS             complement(10643..11344)
                     /locus_tag="VNU03_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76552.1"
                     /translation="MRQFMSRFIVRRLAALALIFVIPVPAAADEAIMGIEQYKTPKAR
                     TLATTHQPLLKQMREEIYNCMPWVGVQKAGIGFRQPKGATEDDRYLSVWIMIDQDEDA
                     RFAALPAERRASAMFSRYGTDLLRRMAALQPLVADPNVQGFSVVLSWLKPGTGGAVQP
                     VNETLALFVDKQTTLGFLGKRVSAGEFTRRAKFSVFDGVQDMGRLPLEVWEDSFVRTF
                     KVANYEAPKGQRCSG"
     regulatory      complement(11406..11485)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11652..12365
                     /locus_tag="VNU03_18680"
     CDS             11652..12365
                     /locus_tag="VNU03_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76553.1"
                     /translation="MLSDAPVAVSLLFVVTVLGMLALVLWGAGRAARRVGPGAMRGVR
                     LAGVALGGWLAITAALAGQGFFEDFYALPPHMLLAIGPPLLALLALTTLGRLDPLLAA
                     LPPSWPIAAQTFRVLVEIVLWRLAVAGVAPEIMTFTGRNVDILVGLTAPIVAYACFVR
                     RAWPARVARWWNWAGVVILLNVVVHAQLSAPTRFRVFETDPPTTFIGDLPYIWLPAFL
                     VPLAWALHAISLRQLRSTR"
     gene            complement(12376..13032)
                     /locus_tag="VNU03_18685"
     CDS             complement(12376..13032)
                     /locus_tag="VNU03_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWP76554.1"
                     /translation="MVLGIDNVVFIAILVARLPDGQREFARRLGLVLALGVRVGLLFT
                     ISWMMGLTEPLVTVLGRGISGRDLILLGGGLFLIFKATREIYEKVEADPHAPAAAGAR
                     AAFFWVLVQILLLDIVFSLDSVITAVGMANHLSIMVTAMVIAMLVMLVSAGGVSRFID
                     QHPSLKILALAFLLLIGVMLVAEGMGTHVEKGYIYFAMAFSLLVEMLNMRYRRRRSAR
                     "
BASE COUNT         1764 a         4745 c         4732 g         1793 t
ORIGIN      
        1 accgcgagtc gatcgtgaag gagctgtggc gcggccgcgg cgtggtgccg aacggcccca
       61 tcgccaaggg cgacaaccac ttcgacgcga gtctgcctcc gctggcctac aacccggcgg
      121 aggcgaaggc gcggctccag aaggcgggct acaagggcga ggagatcttc atcgagacca
      181 ccgtcgccta cgtggcccag gacaaggcga tggcggaagc catcgcggcc atgtggaagg
      241 acgtcggcgt caacgtgaag gtcgaggtca tcgagtactc ggtgcgggcg cagaagaacc
      301 gtgagaagag cttcaagggc gtgttctggt cggacccgac gtcgaccctg agtgacccgg
      361 acgggatgat gtggcgcctc ctggggccgg ggggaccgca agactactgg cgcgaggcca
      421 agttcgacga gctgggtaac gcggcgcgct tcagcgtcga cgagaagttc cgggggacgg
      481 cgtacaagga catgaccaag atcttcctgg agcacctgcc gtggctgccg gtcatccagc
      541 cctacgagga ctacggcatc cagaagtaca tcgacttcac gccgaacccg aaccagcagt
      601 tcgagatccg acgcttcaac ttcaagttcc gccgcgcgta gagtcagtcc ccctcaccct
      661 gccctctccc ccggaaacgg gggagagggt ggcggcgagg tccctgctat gcgcggattc
      721 gtcccgttcc tcggccagcg cctcttccgc gccctcatcg cgctctggct cgtgtccacc
      781 gtggtcttcg tggtcatgcg cctgtccggc gatccggtgc cgctgctgct gccccccgac
      841 gcacccacca gcgagatcct gcgcgtccgg cgcgagctgg gcctcgacaa gccgctgccg
      901 gcccagtacg tgatcttcct cggcaacctc gcccgcggcg acttcggccg ctcgatccac
      961 ttccgcgagc ccgccttcaa cgtggtgcgc ggctacctgg ccgccacgct cgagctgggg
     1021 ctcacctcct tcgtcctggc cgccctgatc gcggtgccga tcggcctctt ctccgcgatg
     1081 cggcgcaaca ccctggtgga ccacgtggcg atgggcctgg ccctgatcgg gcagtcggcg
     1141 cccaccttct tcctcggcat cctgttcatc ctgctgctgt cgctcaaggc ggatctcttc
     1201 ccgacctccg gccgcggcga ctggcgcaac ctggtgctgc ccgcgctgac cctcggcgcc
     1261 ttcacgatgg cctcgatcgc gcggctgacc cgctccgcgg tcctcgaggt cctgggggcc
     1321 gactacatcc gcaccgcgcg ggccaagggc gtggcggagg cgtgggtggt ggccaagcac
     1381 acgctcaaga acgcggcgat cccgatcgtg accatcaccg ggctccagtt cggcaccctg
     1441 ctgggcggag cggtcgtcac cgagaccgtg ttctcgtggc cgggcatcgg gcgtctcgcg
     1501 gtgcagtcga tctacaaccg cgactacgcg gtggtgcagt gcaccgtctt cctggccgcg
     1561 ctcatgttcg tggtgatcaa cttcttcgtc gatctcatct atggcgtcct cgatccccgc
     1621 atccgcgccg gctgacgcgg tccttcccgc gcccgtcgtg cggccgaagg cgcgccggcg
     1681 tcggaccccg gtcctcgtgt tgctcggggt cggcttcgtg gcggtgctac tgctggtggc
     1741 ggccgccgcg cccctgctcg caccgcagga cccgatccgc cagagcctgc gcggccgcct
     1801 cgcggcgccc acctggaacg gcagcgacgg gcgggcccac ctgctgggaa cagatcacct
     1861 cggacgcgac gtgctctcgc gggtgatctt cggatcgcgg gtctcgctcg tgatcggctt
     1921 ctccgcggtg ctggtgggcg gtgtgctcgg ctcggcggcg gggctggccg ccggcttcag
     1981 ccgcgggcgc atggacgcga tcatcatgac ggtggccgac gcgcagctcg cgttcccgtt
     2041 catcctgctc gccatcggca tcatcgcggt gctgggcccg agctttccca ccctggtggt
     2101 ggtgatcgga ctctcgggct gggtgtccta cgcgcgcatc ctgcgctcgc aggtgctggt
     2161 gctgcgctcg cgcgagttcg tggagtcgat ccacgccctc ggcggctcgc tcgcgcgcgt
     2221 cgtgctccgc cacgtgctcc ccaacgtgct ctcctccatc gtggtcatcg cgacgctcga
     2281 gctggcgcgg gccatcgtgc tcgaggccac gctctcgttc ctgggcctgg gcatccagcc
     2341 gccgaccccg tcgtggggcg ggatggtgca ggaggggcgc gagtacctcg actcggcgtg
     2401 gtggatctcc acctgtcccg ggatcgtgct catggtcacc tccatcgtgg tcagccgcac
     2461 cggcgactgg ctgcgcgacg tgctggaccc gacgctccgc ggggagtagg gccgaaagga
     2521 tctccttcat gccggtcacc gctttgatcg agtacggggc ggccagcccc gaggtgcgcg
     2581 cggtctacga cgacatcatg gccacccgca agacggactg gatcaacaac ttctggaagg
     2641 cgctcgccaa ccacccgccc acgctgcgcc gcacctggga gtccatcaag cagatcatgg
     2701 gcgcgggcgc gctcgacccg ctcaccaagg agatggtcta cctcgcggtc agcgcggcca
     2761 acggctgcga ctactgcatc gcctcccaca ccgcgggcgc acgtcgggcc ggcatgagcg
     2821 agacgatgcg gggcgagctg ctcgcggtgg tgggcatggc ctgcgagacg aacgcgctcg
     2881 ccaacgcctt tcaggtcccg gtggacgagc ggttccgcga tccggcctga ggatcggccg
     2941 ggatctcgcg ctcgaggcgc agatcggcca gggtgtgccc ggccagcgca gcgagcacca
     3001 ccgcgccgcc caccagcgac ggcgcccccg ggttctcccc gagcgcgagc cacacccaca
     3061 ccggccccag cacgctctcc agcaccgcga tcagcgaggc ctcggccacc gggatgcgcc
     3121 gcgcgcccgc ggtgaacagg atcatcccga gcccgagctg gcccgccccg aagagggcca
     3181 ggagggccag gtcgcccgcc cccgcggcgc gtggggtcgc cgacccccac gcgatcgcgc
     3241 tcgtgagcgc ggcggccagc gcggcggcgg gcagcatctg cacctgacgc cggcggcgca
     3301 ggagcacggt ggcgagcgcg aaggtcagcg ccaccgcgaa ggcgagcagg tcgccgccca
     3361 atgactcgct ggcgggcgtg gagtagaaat agcgcgagac catgaccgcg gtgccgagca
     3421 gcgcaacgcc catcgcgagc caggtgcgac cggccacccg ctcgcccatc cagatccagc
     3481 cgagcagccc cgccacgaac gggctgaggc tctggatgat caggacgttc gcgaccgagg
     3541 tgtggcggag cgccatgatg aagcacgtgg agccgatggc gaagcacgcc gccatgccga
     3601 ccccagtgcc gcccatgccc gcgaagaccc ccggggtgcg ccggccctcg cgcaccgcgg
     3661 tcaccgcgac caggaacgcg gcgcagaaga gcccgcgcca gaacacggtg gtccagggat
     3721 cggtgccgac gttgcgggcc atcagcccgc ccaggctcca ggagacggcc gccgccgcca
     3781 ccagcagacg gccgcgccga cggaaagcga cgccgtccgc gatcgcggcg cggctcaccg
     3841 ggcgagcgcc acgcaggcca cgccggccag caccaccgcg gcccccgcga gccggcgcat
     3901 ccccgtgtcc tccttcatcc acgcggcgcc gatgatggcg gagagcacga tcgacatctc
     3961 gcgggccgcg accacgtagc cggtcttcga gagctggaag gcgaagagca cgagcaggta
     4021 ggcggtcggg ctcatcagcg cggcggccac gatggtcgcg cggttgcgcc gccattcccc
     4081 gccgagcgcg tcccggcggg cgagcgccac cggggcgagc aggacgcagg agccgagctc
     4141 catcagccag atgtaggcca gcgggctcat gcgggagacc ccggcccggt cgagcagcga
     4201 gtacgccgcg atcagcacgc cggtggcgag cgcccagccg gtgcccgagc ggtgccgcgg
     4261 ggcgacgctc tccgcgccgg ggcgcccgcg caggccccag tgcacgaaga agatgccggc
     4321 cgccaccagg atcaccccga gcgtgccgag gggcgacagc cgctcgtcga agagcgcgaa
     4381 ggcggccatc ggcacgagcg cgaccccgag cccgcgcgcg atagggtaga cgagggagta
     4441 gtcgcccgcc gcgtaggcgc gtccgagcga gaagaagtag agcgcgtgga ggaggacggt
     4501 gccgatcacg aacgggatgg cggcggcccg gaagccctcg cgaccgagca ccgcgagggc
     4561 gattggcgcg tagagcccgg aggcgaccac ccccacccac cagaggaaca gcaccggctc
     4621 accggcgcgc ttggcgagtg cgttccaccc cgcgtggagc acggccgcgc cggcgacgag
     4681 cagaagggcg gcgacggtca ccgggacagg gtaggggttt cccccgacga cgccaagcga
     4741 ttactatgga gagcgcatcc atggcctatt ccctccggct catcgaggac cggctcgcgg
     4801 cgaatgcggc cgctcccgtc tctcgcgctt ctcgcgtgct ctacgcggtc gagggcgcgc
     4861 tgctcgccga cgggatctcc gagccggcgc ccgcggtggg ccccggcgcg gcccgcgccc
     4921 gggccgccac gaccgcggcg cgcgtctacc gggtcgagct ggtgcggcag ccgcccccgt
     4981 ccgcgggcgg gcgggtcctg ctggagcatc cgatcgacct cgcgggcggc tcgggctggc
     5041 tcatgcgctg cgaccgggtg gacttcgatc cgggtggcgt cgcgccgcgg caccggcacc
     5101 agggcggcgg catccgctgt ctcctgcagg gccgtctcga ggtgacggtg ggcgagagcg
     5161 cgccgcgcgc ggtcgtgccg ggcgaggctt ggttcgagag cgggcgtgag ccggtgctgg
     5221 ccgtcgccgc gcgcgatgcg gagacgagct tcatccgggt ctcgatcctg cccgccgcca
     5281 ttgccgggcg ctcctcgatc ctctacgtgg atcccgccgc cgcggcgggg ggccggccgc
     5341 ggcgctacac cgtctacgtg gacgagccga tcgccctctg acgatgcgcg tcctgatcct
     5401 gaccgccagc tacggctccg gccacgcgga ggccgcgcgc agcctggccg gcgccttcgc
     5461 gagccgcggg gccgagccgg tggtggtgga tcacttccgc gagctggtgc acccggtctt
     5521 cgcgcggctg agcggcgcga cctactactg gctcctgcgc cacgccccgt cgctgtgggc
     5581 cctcggctac tcgctgggag accgcctggg ccccgagtcg ccgctcgcgt tcggggtgcc
     5641 gagcctcggc acggcgagcc tcggccgcct gctcgacgcg atgcagccgg acgcggtggt
     5701 gaccgtccac gccacccccg cggcggcgat ggcggcgctc gccgcctccg ggcgcgcgct
     5761 gccgccgcac accacggcgg tgaccgactt cgtggcgcac agccagtgga tgccgcgccc
     5821 ggtggaccgc tactgcgtgg ccgcggagga ggtgcggaac gagttcgtgg cgcgcgggat
     5881 cccccccgat cgcgtgatcg tgaccggcgt cccggtgcgc gaggagttct cgcgtccggt
     5941 cgagggggcg gcggcccgcc gcgcctggga cctcgccccg cggctgccca ccgtggtcgc
     6001 gatggcgggg acgcagggct cgctcggccg gctgtacgac gtcgtcctcg cgctgctcac
     6061 cgtcgagcgc ccgctgcagg cggtggtggt ggccgggcgc gacgagcgcc tgcgcgcgcg
     6121 gctcgcccgc ctcgccgcgg gcagccgggt ccgggtggtg ggctacgtgg aggacgtgcg
     6181 cacgctgctg gccgcggcgg atctgctcgt gaccaaggcg ggcgggatga ccctggccga
     6241 ggcgaccgcg accgaggtgc cgctgctcct ctacggaagc ctcccgggcc aggagatcgg
     6301 caacgagcgc ttcgcctccc gcgccggcat cgcgctggtg gcccgctcgc gcggcgagct
     6361 ccgccgcctg ctcgaccgcg cgctcgccga tccggagacc gtcgagcgga tgcagggctc
     6421 gctgcgccgt ctgaggcgcc cggacgcggc ggcccacatc gtcgacctcg tcctcgagca
     6481 gatcgcgacc cgccggaggg gctgagtgtc gcagttcgcc ttctgcctcg agagcggcga
     6541 tcgcgcgctc cgggaggggc gctacgcgga cgccgagcag gcgctggcgg cggcgctggc
     6601 gctcgatccc cgcagcgtca aggcccagtt caagctcggc ctcacgctcg agcgccagcg
     6661 caagtgggcc cccgcggaga ccgccttccg ggcggccatc cggggcaagc gcgatcacgc
     6721 caccgcgtgg ctgcggctct ccgcggtgtg ccgccgcctc ggcaaggccc gcgaggagga
     6781 gaccgccgcg cgcgaggcgg tgcgcctggc cccgcgcagc agcgaggcgc accacgggct
     6841 cgcggtgacc ctcgcgcgcc tcgggcgcgc cgaggaggcg gtggccgcct tcgaggagac
     6901 gctccgcctg gccccctcga gcgcggtcgc ccacggcggg ctcggcgcgg cgctcaccac
     6961 cctcggccgc cacggcgacg cggcagccgc cttcggccgg gccgcgcgcc tcgaccccgc
     7021 ctcgtacaag gcgcacctgc agcacgcgga ggcccaccgg cgccagcgcc gatggggcga
     7081 ggcggtggac gcctaccgcg cggcggccgc gctccggccc gcggtgcccg cgctgcacgg
     7141 gcgcctcggg ctcatgctcg agcgccagcg ccgctaccgc gaggcggtgg ccgcctacac
     7201 ccgcgcggcc cggctcgaca ccgccggcgc ccgctggcgc gcgcgcctcg gcggcgtgct
     7261 cgagcggcag ggccgttacg gcgaggcgga ggccgcgctg ctcgaggcgc tgcgccggga
     7321 gcccgaccgc ctcgacgcgc acctgagcct ggcgatgatc taccgccgcc agggcaagtg
     7381 gggcgacgag gcgcgcgtgt acgagcgggc catcggcctg aagccgggcg acgcccatct
     7441 ccacgccgac cttggtctgg ccctcgaggc ccaggggctg ctcgggcccg ccgaggggca
     7501 gttccgccgg gcgctcgcac tcgcccccga gaacgccgac ctccacgtcg agctcgggat
     7561 cgcgatgggc cgccagggcc gccacgcgga ggcggaggcc gcgctccgcg aggcggtacg
     7621 cctcaatcct caggatctgg tggcccgcgc gaacctcgcc ctcggtctcg ggcgccaggg
     7681 tcgccacgcg gaatcggagg cggcctaccg ccaggcgctc tcgctcaagg cggacgccgc
     7741 gccgctccac cgcgggctcg gcatggcgct gtactcgcag gccaagcgta ccgaggcgga
     7801 ggccgccttt cgcgaggcgg tccgtctcaa gccgagctac gcgcgggcct ggggcgacct
     7861 gggcgagctg ctgaccgaga ccggccgcca cgacgaggcg gtggaggcct tccgggaggc
     7921 catcgcggtg aagcccgggc gcatcgcctc gcaccggggc ctgggcaccg cgcgcctcct
     7981 gcaggagcgt cccgacctgg ccgagaccgc gttccgcggt gcgctcgccc tccgcccgga
     8041 cgactcccag agccacttcg gcctgtggac cgcgctcgag cggcagggaa agctcgtgga
     8101 ggcggaggcc gcgtaccggg acgccatcgc gatgagcgcg gatctggcgg aggcccactc
     8161 ccggctcggc gagctgctgg cgcgtcaggg gcgctggcgg gaggccgaga ccgcctatcg
     8221 cgaggcgctg cggcggtcgc ccgacgacgc gcgctcgcgc gtcgcgctca ccgcggtgct
     8281 gcgccgggcc cgcggctagg tatcggtggg atcgacttcg atgttggtga tgcggaggta
     8341 gctgaccgcc acccgctcgt cgccctgctc cagcagcacg ccgtcgtcgg cgatctgggc
     8401 aaggctgccc ttgatgggct cggtggagtt cttcaccgag atgagcaccg cccggcccag
     8461 gtgacgctgg gcctcggcct gccacatcgt cttctgcacc tcgtcgaaga tcccggcggc
     8521 ctggcgggcc gccgaggcgg cccggctcgc cgcggtgatg gtgccgaggc cggggacctg
     8581 caggccgccc ggaaagaccg cggccgccgg attgctgccg gtcgcggccg ccacgggtcg
     8641 cgccaccatg tcgccgccga ccccggggac cgcggaggcc gcggcgccgg cgccggctcg
     8701 tccaccggcg tcctgctgct cctcgaggta gcgcagcacc tcggacagcc gcgccatcag
     8761 gttcgagaag atgaagagcc cgacgagcgc gcaggccagc gccaccccga gccccatctg
     8821 ggcctcggtc agcttgagct ggttcagcgc gtagagggtg taggtgaaga gcaggagggg
     8881 aatgacggcc gtcagcgcga acacgatcga ggccgcgttc cggagggtat aggtccgccc
     8941 tctactcatg gtgtcccaaa tctagcgcgc ccggcctcgc gccccaagtt ttgagagagc
     9001 cggccccggt ggccgccccc cgggcctcgg gcccgcgtga tagtatccga ccgcaaccat
     9061 gagccccagc cggcgcaccc tgcgcgacat gcgtggcttc gtcttcgacc tcgacgggtg
     9121 cgtctggacc ggcgacgtcc tcactcccgg cgccgcgcag gtcctcgccc tgatccgcca
     9181 gcagggcaag gcgctctgct tcctgaccaa caactcgcgc gcgcgggccc ggaccatgca
     9241 ggccaagctc gtgcgtctgg gggtggaggc caaggagcac gaggtgctca cccccctcga
     9301 gatcctgggc gaggtggtga ccgagcgctg gggtccgtcg agcgtgctgg cgatcggcgg
     9361 ccccgagctg gcggaggtgc tggtcgaggc cggccaccgc gtggtcccca tcgaccgctc
     9421 ccgcgacgcg acggtggtgg tggtcggcaa cgacttcgac ttcagctacg agcgtctcac
     9481 cgcggcggca cgcgcggtgg ccggcggcgc cgcgttcctc actccgaaca tcgacccgcg
     9541 actgcccatg gagaacggcg agttcctgcc cggctgcggc gccatcgtcg aggcggtggc
     9601 caccgcggcg ggcctgcgcc cgatcgtggt gggcaagccg gagccgcccc tgttcgagct
     9661 ggccctcaag cgcatgggcc tgggggtcga cgaggcggcc atggtgggcg acagcgtgga
     9721 ctcggacgtg cgcggcgccc gccgcgtcgg catgaccgcg gtgctgttcg cgcccaaggg
     9781 tggcctggat ggcgtggcgc actatctggt caagtcgatg tcccagctga gacggctgct
     9841 gggcggatag ggatgcgcgt cctcctgcac tcgggcggcc cgctggggcc gctcggccgc
     9901 gcggccctgc ccgatttcct gggcccgctc cgccgggtgg ccttcgtgac cgcggcgagc
     9961 ctgcacgacg agaccgcctc gttcgagcgg gtgcgggccg cactcggcgc gccgcccccg
    10021 gatggcgcgg gcctcgagct gctgcacctg cgctggaacc acgatccgct cgagacgctg
    10081 gccggggcgg aggcgctctt catgggcggc ggcaacacct acgcgctgct ccagcggctg
    10141 cgcggggccg ggctgctgga ggccattcgc gcccgcgtgg ccgcgggcat gccgtacatg
    10201 ggcgcgagcg cgggatccaa cgtggccggt cccaccatcc tcaccaccaa cgactggaac
    10261 gtggtggccc tcgatcgctt cgacgccctc gggctggtcg gcttcaacat caatccccac
    10321 tataaggaag ccgaccccgc gatggcgccc tacagcgaga cgcgcgacga ccggatccgc
    10381 gagtaccacg tggtcaacgc caatccggtg ctcgggctcg aggaaggcgc gtgggtgatc
    10441 gtcgaggacg gggtggtgac cgcgcacggg accgcgcggg tgaagctgtt ccggccggcc
    10501 gccgacccgg tgtggtaccg ggccggcgac cgtctgccgg ttacttgacg accgcccgga
    10561 aggacccgcc tgcttcgctc gtcgtcgcat cgggtgcttc cgagcggtct ggttcgtcag
    10621 gggtcagggg agcggtcggg cgtcagcccg agcacctctg gcccttgggg gcttcgtagt
    10681 tggcgacctt gaaggtccgg acgaaggaat cctcccagac ctcgagcggc agccgaccca
    10741 tgtcctgcac gccgtcgaag accgagaact tcgcgcggcg agtgaactcg cccgccgaca
    10801 cccgcttgcc gaggaagccg agcgtggtct gcttgtccac gaacagggcc agcgtctcgt
    10861 tcaccggctg caccgcgccg ccggttccgg gcttgagcca cgagagcacc accgagaagc
    10921 cctggacgtt gggatcggcc accagcggct ggagcgccgc catgcgccgg aggagatcgg
    10981 taccgtagcg ggagaacatc gcggaggcgc ggcgctccgc gggcagcgcg gcgaagcggg
    11041 cgtcctcgtc ctgatcgatc atgatccaca ccgagagata acggtcgtcc tccgtcgcgc
    11101 ccttgggctg acggaagccg atgcccgcct tctgcacgcc gacccacggc atgcagttgt
    11161 agatctcctc gcgcatctgc ttgaggagcg gctggtgggt ggtggcgagg gtgcgcgcct
    11221 tgggcgtctt gtactgctcg atacccatga tcgcctcgtc ggcggccgcg ggaaccggga
    11281 tgacgaagat gagcgcgagg gcggcgagcc ggcgaacgat gaagcgggac atgaactgtc
    11341 gcatcgggat cctcctgtcg cggaacgcat ttcagatccc gacgagcgcc ttcgtgcgcc
    11401 tttccttcgg tagcccggct gtcaggaatt cacctgaccc gcggctttgc gtccccgtct
    11461 cacgacgggt ttgccattgt cgggaagaag ctccgcgagg aggagagggg agcaatcgcg
    11521 atgccagcgc gcggggcgcc cgggcggcca gcaatgatcg gtagatagcc ggcccgcgcg
    11581 gagcgcggtg tcatcggcct gacgcgcgtg tcggcatacg ctcacacgca agatgtgcta
    11641 tccatgcggg catgctctcg gacgctccgg tcgcggtcag cctgctcttc gtggtcacgg
    11701 tgctgggaat gctggcgctc gtgctgtggg gcgcgggccg cgcggcgcgc cgcgtcgggc
    11761 cgggcgcgat gcgcggggtg cggctcgccg gggtcgcgct cggcgggtgg ctcgccatca
    11821 ccgcggcgct ggccgggcag gggttcttcg aggacttcta cgcgctgccg ccccacatgc
    11881 tgctcgcgat cggcccgccg ctcctcgcgc tcctggccct gaccactctc ggtcgactcg
    11941 acccgctgct cgccgcgcta cccccgtcct ggccgatcgc cgcgcagacc ttccgcgtgc
    12001 tggtggagat cgtgctgtgg cggctcgcgg tggcgggcgt ggcgcccgag atcatgacct
    12061 tcaccgggcg caacgtggac atcctggtcg ggctcaccgc cccgatcgtg gcctacgcct
    12121 gcttcgtgcg gcgcgcgtgg ccggcccggg tggcgcggtg gtggaactgg gccggggtgg
    12181 tcatcctgct gaacgtggtg gtgcacgcgc agctctccgc gccgacgcgc ttccgggtgt
    12241 tcgagaccga cccgcccacc accttcatcg gcgacctgcc ctacatctgg ctccccgcgt
    12301 tcctggtgcc gctggcctgg gcgctccacg cgatctcgct gcgccagctc cggtcgaccc
    12361 gctgatcggc gcgcttcacc gggcactccg acggcgccgg tagcgcatgt tgagcatctc
    12421 cacgagcagg gagaaggcca tcgcgaagta gatgtagccc ttctccacgt gggtgcccat
    12481 gccctccgcg accagcatca ccccgatcag gagcaggaag gccagggcga ggatcttgag
    12541 gctcgggtgc tggtcgatga accggctcac cccgcccgcc gagaccagca tgacgagcat
    12601 cgcgatcacc atcgcggtga ccatgatcga caggtggttg gccatgccga ccgcggtgat
    12661 caccgagtcg agcgagaaga cgatgtcgag cagcaggatc tgcacgagca cccagaagaa
    12721 cgcggcccgc gcccccgcgg cggccggggc gtggggatcc gcctccacct tctcgtagat
    12781 ctcccgggtc gccttgaaga tgaggaagag gccgccgccc agcaggatca ggtcgcggcc
    12841 ggagatgccg cggccgagca cggtgacgag cggctcggtg aggcccatca tccacgagat
    12901 cgtgaagagc agcccgacgc ggaccccgag cgcgaggacg aggccgaggc gccgcgcgaa
    12961 ctcgcgctgc ccgtcgggga ggcgggccac caggatcgcg atgaagacca cgttgtcgat
    13021 gcccagcacg atct
//
LOCUS       DATMCV010000814        13138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_643060, whole genome shotgun sequence.
ACCESSION   DATMCV010000814 DATMCV010000000
VERSION     DATMCV010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643060"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VNU03_18690"
     CDS             complement(<1..982)
                     /locus_tag="VNU03_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76555.1"
                     /translation="MSRLVLTDVSRRFGTVTAVDRFSLTLQPGEFVSLLGPSGCGKTT
                     TLRMIAGFLEPSEGTIDMDGRRISAPGSTLPPEKRNMSMIFQSYAIWPNMTVAENVAF
                     GLEVRRVPKDELRIKVQRILDVVQMGHLGGRYPQELSGGQQQRVALARAIVVQPAVLL
                     LDEPLSNLDANLREEMRYEIRRLHDEFQITTVYVTHDQAEAMVTSDRIAVMHQGRIEQ
                     VDAPRALYARPRTRFVAGFIGRTNLLGGLVRGEDVVFDHFTIARDRFAEAAALQGQVS
                     FSLRPQSIHLYRLSPPVSDRVCVVPGVVAQRAYLGEHWDYTVRPAGSALAL"
     gene            complement(979..2655)
                     /locus_tag="VNU03_18695"
     CDS             complement(979..2655)
                     /locus_tag="VNU03_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWP76556.1"
                     /translation="MREKSFVIWLLLIALLLLLVINPLARLLWASFQHPETGAFTLAN
                     YAAAYGRWRHVEALINSLVIGLAVAALCTVFGVPMAWAVSRTDMPGKGLVWAAVLGTF
                     IMPNYLGAVAWILMAGPNAGWLNRVYTAITAADAGPFNVYSKTGLVLVIACYSFPYMF
                     VFTRSALDLISSEMEDAASTLGAGALRTTLRVTLPLALPAIMGAFIVEFLESIALFGA
                     PALIALPARFQVMTTTLWQMFEFPPRVQEAAAYAMPLLLITVFLFWLQYRIIARKGYV
                     ALTGKGGERRLVRLGPWRFILLGYCLFVTSLSFFLPMLVVLQAAFARAWGRGFSWDNV
                     TLRNLRFVLFDQTATRAATLNTFVYAAAASVIALTLALAIAYIVARRLVPLGRALTYV
                     AMAPFVIPGIVLAIGFYAAYTTKPLVLYGTAWILILAFATRFLPIAYTNSDAALRSIN
                     PELEDAARILGAGRLLSLRRIVAPLLKRGLAGAFILVFIPATRELSSAIFLYTTGTQV
                     LSVLLFDKSDEGNFEVLSSMGLILVVVTVALVLVGFRMVGRDFLLRRTAT"
     gene            complement(2672..3706)
                     /locus_tag="VNU03_18700"
     CDS             complement(2672..3706)
                     /locus_tag="VNU03_18700"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP76557.1"
                     /translation="MARLAALALTLTLVLAGSPAALAFDGEPALYEAAKKEKEFTWYT
                     AHYNSEAAAAICQGFEKKYPGVKCNYIRTTAQVAYQRLAQDQKAGIAQCTVISSTDQS
                     HYTKMKQDGWLLPYKPKNLSELVDAFRSFNDPDGLFTATAAGLVLLTYNTSVVSPAEA
                     PRKWTDLADPKWKNKVSIGHPGFSGYVGTWVVQMKKLYGWDYFKKLELNKPRIGRSIN
                     DTVTMLNAKESIVAAGPSATTLESRAKGNPVAVVYPEDGAVLMVSASGILKNAPAPNA
                     GRLFIEYLLSREGNEVMVAQYQDPVNKGVKPMAGGKSIAEIKTIRPTAVEIEKGIPEV
                     KELFRETFGI"
     gene            complement(3712..4530)
                     /locus_tag="VNU03_18705"
     CDS             complement(3712..4530)
                     /locus_tag="VNU03_18705"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWP76558.1"
                     /translation="MGYEQILYEVRDRIATVTLNRPDRLNAWTGVMGREVRQAMDDAA
                     RDEAVRVIVLTGAGRGFCAGADMQMLSGIVDAGTAARVEETGPAVDGAIRDDFRGPYA
                     YFPMVPKPIIAALNGATAGLGLVVSLYCDLRIAADRAVFTTAFSRRGLIAEHGVSWML
                     PRLVGLQHGLDLLLSGRKFTAAEALAMGLVSQVHPAGVLMDAVRSYGGELADLVSPRS
                     IRVMKRQIWEAQFQTLAEATAVGHEEMRQSFGAEDFKEGVAHFVEKRAPRFTGR"
     gene            complement(4613..5863)
                     /locus_tag="VNU03_18710"
     CDS             complement(4613..5863)
                     /locus_tag="VNU03_18710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP76559.1"
                     /translation="MDAPVSDRIGDRAAGADTRWRLLAILTTSYGAGAFGMLGISPLS
                     PSLVEGLGLTRFQVAFIVPSIYLAGLLFSLPGGRLADRLGVRPAFLSGLALGAAGLLA
                     AALAPGFLAFLACLFVAGAGWSVVNPTLGKAIMDVFPARERGIAMGVKQMGLTLGGLI
                     SALVLPAIAATLGWRYAVGACALVVALPVALGWRPLGAFRAGGRAASPGELGATGSSW
                     WWARRPALVIFFATGFVLGMVQAAVLSYLPLFTIQALGFDKIGAGLLVAASQAGGAVS
                     RLALGAASDRWFTGQRSVWLAGTGAVGAVIFALYAAWPATSPLAAGLLAFVTGIGAYG
                     WVGIFFVISAEAGGPREAGLLSGVAFASIVLGLLVGPPVFGLLLEAANSYSMAWAVFA
                     ALSALVAIVALLAGPAIDRASHHR"
     gene            5862..6212
                     /locus_tag="VNU03_18715"
     CDS             5862..6212
                     /locus_tag="VNU03_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76560.1"
                     /translation="MKIHHLFVDQKGESHFEDMEIPYVETGRAGRLSARLPATGIIFR
                     EVQPDYDLDWHPAPRRQYIINLDAGVQITASDGEARVIGAGEVILVEDTWGKGHLSKA
                     LDGKLRHCIFVPIE"
     gene            complement(6338..7504)
                     /locus_tag="VNU03_18720"
     CDS             complement(6338..7504)
                     /locus_tag="VNU03_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HWP76561.1"
                     /translation="MATVSDRQRELRNVACIVGVDESDQIGTLPNVSQLSLHLEAISN
                     AVRDAGLQVKDVDGIFTAGQHSPATLGEALGVKPRYVDGTTVGGCSFIIMVGHAVAAL
                     HHGLCDVAVVSHGESGRSGTGVTRSRDTALSGQFEFPYGFGGATSYFGMITTRHMHDY
                     GTTLEQWAQVAVSTRKWAALNPKARFRDPITVADVLNSRPVSYPFNLLNICLVTDAGG
                     AVVLTRADRAKDCAKAPVYVRGFGEATEHVMVTQMDSLSVSAATRLAGERAFSMAGVS
                     GKDFNHIMLYDAFTSGPPIMLESLGLAKPGEGVQFFSEGRSTPGGSLPINTNGGGLSY
                     THSGMYGIFPIIEATRQLRGETGARQVPGASCSLVNGMGGMLSAAGTLVLANRT"
     gene            complement(7510..7926)
                     /locus_tag="VNU03_18725"
     CDS             complement(7510..7926)
                     /locus_tag="VNU03_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HWP76562.1"
                     /translation="MDVDRPLPTPITPEAKPFWDGCRAEKLMLPRCEDCGHVFFYPRI
                     LCPRCHSRRIGWMQASGRGTLHSFEIAHQAFQKAWKIKPPYVLAMVQLEEGPRMMSNL
                     INVEPSPKAIKCDMPVEVVFTKVSDDVTLPLFQPRR"
     gene            complement(8073..8384)
                     /locus_tag="VNU03_18730"
     CDS             complement(8073..8384)
                     /locus_tag="VNU03_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76563.1"
                     /translation="MKRSTIFAVIAVCSMGAVATTVSAAELTNPTPTTEVEEAKMPAG
                     AEFSNGPAGGGATLGTKDHMPSGAEFSNGPIGGAASDPTPRRMPSGAEFSNGPTGISK
                     E"
     gene            complement(8458..8769)
                     /locus_tag="VNU03_18735"
     CDS             complement(8458..8769)
                     /locus_tag="VNU03_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76564.1"
                     /translation="MRRGQPFPFWVAVLAVGLLGLLSPLAHTCTADPRWLVSSADNLD
                     LDDQLLLAADGVLVSALAIPPAPRGTPHLIPELGTGSASSDPGSASRTRAPPVLLARS
                     V"
     gene            complement(8785..10704)
                     /locus_tag="VNU03_18740"
     CDS             complement(8785..10704)
                     /locus_tag="VNU03_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HWP76565.1"
                     /translation="MRLRLAVAVLTLASLASAQPDGGSEWPHYGNDAGGARYSPASQI
                     DRTNVAQLQVAWTFRTGALGHSAPLDRKAAFEATPILVDGKLFLSTPYNHVIALDPRT
                     GARLWEYDAALDRSHDYSEVTSRGVAAWRDTAASPGAPCRTRILMGTLDARLIALDAE
                     TGTPCAGFGASGQVDLTRGVDLRDLGNYQMTSAPAVSGDVIVVGSSIGDNRAVDVERG
                     IVRGFDARTGALRWTWDPIPWASRTTPRTGAGNAWSTLSVDAERGLVFVPTGSASPDF
                     WGGTRKGDNRWANSVVALRAATGELVWGFQVVHHDLWDYDVASQPTLFSWRDGTPAIA
                     ITTKMGRVFVLDRVTGAPLLPVEERPAPPSDVPGEEAWPTQPSSGISVVPESLRAEDA
                     WGSTEKDRAWCREKIAAARSEGIFTPPSLRGTVVFPGNVGGVNWGSSAYDPTRHLLFM
                     NTNRLATLVRLIPHERLALEATQGTSAERLRAELALQAGTPYAMRRAPLLSPGGTPCN
                     PPPWGTLVAVDLFSGRTAWDVPLGSVISGDFKRLLVRLFRPALLDSGSPNLGGPIATA
                     GGLVFTAASMDNHLRAFDSDTGRELWKYELPAGGQATPMTYRVDGRQYIVIAAGGHGK
                     LGTKLGDYVVAFTLP"
     gene            complement(10727..10909)
                     /locus_tag="VNU03_18745"
     CDS             complement(10727..10909)
                     /locus_tag="VNU03_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76566.1"
                     /translation="MNHWMIYSTCSIFAFCAAGAMAVSSPTVDLDALPPTDAADLGND
                     LMDDAITIASPRPTPA"
     gene            complement(10984..11301)
                     /locus_tag="VNU03_18750"
     CDS             complement(10984..11301)
                     /locus_tag="VNU03_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76567.1"
                     /translation="MRRPPPDRFWVALLTLVLIVVLRPLAQASPDDPLWIAGFYDGAD
                     FDDVVSFLVTYSGLDTGRPAIHRPVPRVPETLVEARDCPTGTADRPSTLTRAPPPSFA
                     PPV"
     gene            complement(11369..12151)
                     /locus_tag="VNU03_18755"
     CDS             complement(11369..12151)
                     /locus_tag="VNU03_18755"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76568.1"
                     /translation="MGQLEGKVALITGASKGIGRVMSRLFAQEGAAVICAARTRDLVE
                     ETAGLVKTAGGRAIAVTGDAATEADVQRMIEAGVRAFGKLDVLINNAGDGGPTKRVQD
                     YTSDDWFYTINSCLTSSYMCIRFAVPEMLKAGGGAIVNISSTAGRRGLPFRIGYCSAK
                     AGQVGMTYGLALELAPHNIRVNAIAPGAVAGDRIDRVIAGQAEVRGVALEDMRRAFVD
                     RAPLKRMSTAEDIATLAVYLCSDAAQNLSGQCIAVTAGEPAV"
     gene            12380..12604
                     /locus_tag="VNU03_18760"
     CDS             12380..12604
                     /locus_tag="VNU03_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HWP76569.1"
                     /translation="MTKKSTKTRILSAVVGLSLVSLAATACGTLGGAAVGAGAGAAVG
                     AGTGYGAGKGALIGTGVGAAAGAIYDITKH"
     gene            12620..12901
                     /locus_tag="VNU03_18765"
     CDS             12620..12901
                     /locus_tag="VNU03_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76570.1"
                     /translation="MTPYKAVTLGIMLAVPLWAGFAAGQTKQSCEQMKAGARQQVEGK
                     VARVDQTQGVVAVTEKDGTTHEFKASKETLQDLKVGDNIEARLRELPKC"
BASE COUNT         2011 a         4424 c         4890 g         1813 t
ORIGIN      
        1 gcagggccag ggcgctgccg gccggccgca ccgtgtagtc ccagtgctcg cccaggtagg
       61 cgcgctgggc caccaccccg ggaacgacgc agacgcgatc ggagacgggc ggggacagcc
      121 ggtagaggtg gatcgactgc gggcggagtg agaagctgac ctggccctgg agggcggcgg
      181 cctcggcgaa acggtcgcgc gcgatcgtga agtggtcgaa caccacgtcc tcgccgcgga
      241 cgagcccgcc cagcaggttg gtacgcccga tgaagccggc cacgaagcgg gtgcgcgggc
      301 gcgcgtagag ggcgcgcggg gcgtccacct gctcgatccg cccctggtgc atgaccgcga
      361 tgcgatcgga ggtcaccatg gcctccgcct ggtcgtgggt cacgtagacg gtggtgatct
      421 ggaactcgtc gtgcaggcgc ctgatctcgt agcgcatctc ctcgcgcagg ttcgcgtcga
      481 gattgctgag cggctcgtcc agcagcagca ccgcgggctg caccacgatc gcgcgggcca
      541 gcgcgacccg ctgctgctgg ccgccggaca gctcctgagg gtagcgcccg ccgaggtggc
      601 ccatctgcac cacgtcgagg atgcgctgga ccttgatgcg cagctcgtcc ttcggcaccc
      661 gccgtacctc gaggccgaag gccacgttct cggccaccgt catgttcggc cagatcgcgt
      721 agctctggaa gatcatcgac atgttgcgct tctcgggcgg cagggtggag ccgggcgcgg
      781 agatccgccg cccgtccatg tcgatggtgc cctcgctcgg ctcgaggaag cccgcgatca
      841 tccgcagggt cgtggtcttg ccgcagccgg aggggccgag cagcgagacg aactcgccgg
      901 gctgcagggt gagggagaag cggtcgaccg cggtcaccgt cccgaagcgg cggctgacgt
      961 cggtgaggac caggcggctc acgtggcggt cctccgcagg aggaagtcgc ggccgaccat
     1021 ccggaacccc accagcacca gcgccaccgt gaccaccacc aggatcaggc ccatggagga
     1081 gagcacctcg aagttgccct cgtcgctctt gtcgaagagg aggaccgaca gcacctgggt
     1141 gccggtggtg tagaggaaga tcgcggagga cagctcgcgg gtggccggga tgaacaccag
     1201 gatgaaggcg cccgcgagcc ctcgcttcag gaggggcgcg actattcgac gtagggacag
     1261 gagccggccg gcgcccagga tgcgggccgc gtcctccagc tccgggttga tgctgcggag
     1321 cgccgcgtcg ctgttggtgt acgcgatggg gaggaagcgg gtcgcgaagg ccaggatgag
     1381 gatccacgcg gtgccgtaga gcacgagcgg cttcgtggtg taggcggcgt agaacccgat
     1441 cgcgagcacg atgcccggga tgacgaaggg agccatcgcc acgtaggtca gcgcgcggcc
     1501 gagcgggacc aggcggcgcg ccacgatgta ggcgatggcg agcgcgaggg tgagcgcgat
     1561 gacggaggcc gccgccgcgt acacgaaggt gttgagggtg gcggcccggg tggcggtctg
     1621 gtcgaacagc acgaagcgca ggttgcgcag cgtgacgttg tcccaggaga agccgcggcc
     1681 ccaggcccgc gcgaaggcgg cctgcagcac cacgagcatc ggcaggaaga acgagagcga
     1741 cgtcacgaac aggcagtagc cgagcaggat gaagcgccag ggacccaggc gcaccaggcg
     1801 gcgctccccg cccttgccgg tcagcgccac gtagcccttg cgggcgatga tgcggtactg
     1861 gagccagaag aggaacacgg tgatgagcag gagcggcatc gcgtaggccg ccgcctcctg
     1921 cacccgcggg gggaactcga acatttgcca gagcgtggtg gtcatgacct ggaagcgcgc
     1981 gggcagggcg atcagggcgg gagccccgaa gagcgcgatc gactcgagga actccacgat
     2041 gaacgcgccc atgatcgcgg gcagggccag cggcaaggtg acccgcaggg tggtgcgcag
     2101 cgcgcccgcg cccagcgtgc tcgccgcgtc ctccatctcg gaggagatca gatcgagcgc
     2161 ggagcgggtg aacacgaaca tgtacgggaa cgagtagcag gcgatgacca gcacgagccc
     2221 ggtcttgctg tagacgttga agggtcccgc gtccgccgcg gtgatcgcgg tgtagacgcg
     2281 gttgagccag cccgcgtttg gccccgccat gaggatccag gccaccgccc cgaggtagtt
     2341 cggcatgatg aaggtgccga gcaccgccgc ccacaccagc cccttgcccg gcatgtcggt
     2401 gcgcgacacc gcccacgcca tcggcacccc gaacacggtg cagagcgcgg ccaccgcgag
     2461 gccgatgacc agcgagttga tcagcgcctc cacgtgccgc cagcgtccat acgcggccgc
     2521 gtagttcgcg agcgtgaagg cgccggtctc cgggtgctgg aagctcgccc acaggaggcg
     2581 ggcgagcggg ttgatcacga gcaggagcag cagcgcgatc aggagcagcc agatgacgaa
     2641 ggacttctcc cgcatcgcgc ggcggccggt ctcagatccc gaaggtctcc cggaagagct
     2701 ctttgacctc cgggatgccc ttctcgatct cgaccgcggt gggccggatc gtcttgatct
     2761 ccgcgatgga cttgccgccc gccatcggct tcacgccctt gtttaccggg tcctggtact
     2821 gcgcgaccat cacctcgttg ccctcgcggc tcagcaggta ctcgatgaag agccggcccg
     2881 cgttgggggc gggcgcgttc ttcaggatgc cggaggcgga gaccatgagc accgcgccgt
     2941 cctcgggata gaccaccgcg accgggttgc ccttggcccg gctctccagc gtggtggcag
     3001 agggccccgc ggccacgatc gactccttcg cgttgagcat ggtcacggtg tcgttgatcg
     3061 agcggccgat gcgcggcttg ttcagctcga gcttcttgaa gtagtcccag ccgtagagct
     3121 tcttcatctg caccacccag gtgcccacgt agccgctgaa gccggggtgg ccgatcgaga
     3181 ccttgttctt ccacttcggg tcggcgagat cggtccactt gcggggcgct tccgcggggg
     3241 agaccacgct cgtgttgtag gtgaggagca ccaggcccgc cgcggtggcg gtgaagagcc
     3301 cgtcggggtc gttgaacgag cggaaggcgt cgaccagctc ggagaggttc ttcggcttgt
     3361 agggcaggag ccacccgtcc tgcttcatct tggtgtagtg gctctggtcg gtggagctga
     3421 tcacggtgca ctgggcgatg cccgccttct ggtcctgggc cagccgctgg taggccacct
     3481 gggcggtggt ccggatgtag ttgcacttca cgcccgggta cttcttctcg aagccctggc
     3541 agatcgcggc cgcggcctcc gagttgtagt gggcggtgta ccaggtgaac tccttctcct
     3601 tcttcgccgc ctcgtagagc gccggctccc cgtcgaaggc gagggcggcg gggctcccgg
     3661 cgaggacgag cgtcagcgtc agcgcgaggg ccgcgagccg ggccatggcg gtcaccgccc
     3721 ggtgaagcgc ggggcgcgct tctcgacgaa gtgggccacg ccttccttga agtcctcggc
     3781 cccgaagctc tgccgcatct cctcgtggcc caccgcggtc gcctccgcga gcgtctggaa
     3841 ctgcgcctcc cagatctgcc gcttcatcac ccggatcgag cgcggagaga ccaggtcggc
     3901 cagctcgccg ccgtaggacc gcaccgcgtc catcagcacg ccggccggat gcacctgcga
     3961 gaccagcccc atcgcgagcg cctccgctgc ggtgaacttg cggccagaga ggagcaggtc
     4021 gaggccgtgc tggaggccca ccaggcgagg cagcatccag ctcaccccat gctcggcgat
     4081 gagcccgcgc cgggagaacg cggtggtgaa gaccgcgcgg tcggccgcga tgcgcagatc
     4141 gcagtagagc gagaccacca ggccgagacc ggcggtggcc ccgttgagtg ccgcgatgat
     4201 cggcttgggc accatcggga agtaggcgta gggcccgcgg aagtcgtccc ggatcgcgcc
     4261 gtcaacggcg ggccccgtct cctccacgcg cgccgcggtc cccgcgtcga cgatgccgga
     4321 gagcatctgc atgtccgccc cggcgcagaa gccgcgcccg gcgccggtga gcacgatcac
     4381 gcgcaccgcc tcgtcgcgcg ccgcgtcgtc catcgcctgg cgcacctcgc gacccatcac
     4441 gcccgtccag gcgttgagcc gatcggggcg gttcagggtg acggtggcga tgcggtcccg
     4501 cacctcgtag aggatctgct cgtagcccat gtggcgcgct ccttcgcggt tgtcgccgcc
     4561 gtcgaggcgg ccgaggccgc atgatagcgc ggctctcctc gggccgccat cctcagcggt
     4621 gatgggatgc gcggtcgatg gcgggaccgg cgagaagggc gacgatggcg accagggccg
     4681 agagggcggc gaagacggcc caggccatcg agtatgagtt ggcggcctcc agcagcaggc
     4741 cgaagaccgg cgggcccacc agcaggccga gcacgatcga cgcgaaggcc acgccgctca
     4801 gcaggcccgc ctcgcgaggg ccgcccgcct ccgcgctgat cacgaagaag atgccgaccc
     4861 acccgtaggc gccgatgccg gtcacgaagg cgaggagccc ggcggcgagc ggcgaggtgg
     4921 cgggccaggc ggcatagagc gcgaagatga ccgcgcccac cgcgccggtg ccggcgagcc
     4981 acaccgaccg ctggccggtg aaccagcggt cactggccgc gccgagcgcg aggcgcgaga
     5041 ctgccccgcc ggcctgcgag gcggccacga ggaggccggc gccgatcttg tcgaagccga
     5101 gggcctggat ggtgaagagc ggcaggtaac tcaggaccgc ggcctgcacc atgccgagca
     5161 cgaagccggt cgcgaagaag atcaccaggg cgggccggcg cgcccaccac cagctcgagc
     5221 cggtcgcgcc gagctccccg ggactcgcgg cgcggcctcc ggcccggaac gcgccgaggg
     5281 ggcgccagcc cagcgcgacc ggcagcgcca ccaccagagc gcaggcgccg accgcgtacc
     5341 gccagcccag ggtggccgcg atcgcgggca ggacgagggc cgagatcagt ccgcccagcg
     5401 tgaggcccat ctgcttcacg cccatcgcga tgccgcgctc gcgcgccggg aagacgtcca
     5461 tgatggcctt gccgagcgtg ggattcacca cgctccagcc cgcgccggcc acgaagaggc
     5521 acgcgaggaa ggcgaggaag ccgggcgcga gcgcggcggc cagcaggccg gccgcgccga
     5581 gggcgaggcc gctgagaaag gcggggcgca ctccgagccg atccgcgagg cgcccgcccg
     5641 gcagcgagaa gagcaggcct gcgagataga tcgacgggac gatgaaggcc acctggaagc
     5701 gggtgaggcc caggccctcg accaggctcg gcgacagcgg cgagatcccc agcatgccga
     5761 aggcgcccgc gccgtatgat gtcgtcagaa tcgccaggag ccgccagcga gtgtcggctc
     5821 ccgcggcgcg atccccgatc cggtcagaga caggagcgtc catgaagatc catcatctgt
     5881 tcgtcgatca gaagggcgag tcccacttcg aggacatgga gatcccgtac gtcgagaccg
     5941 gccgcgcggg ccggctctcg gcgcgcctgc ccgcgaccgg gatcatcttc cgggaggtcc
     6001 agcccgacta cgatctggac tggcacccgg cgccgcgccg ccagtacatc atcaacctgg
     6061 acgcgggcgt gcagatcacc gcgagcgacg gtgaggcgcg cgtcatcggc gcgggcgagg
     6121 tgatcctcgt cgaggacacg tggggcaagg gtcatctctc gaaagccctc gacggcaagc
     6181 tgcgccactg catcttcgtt cccatcgaat agggggccga gaagggtcca gatgcgaggc
     6241 gacgccctcg gccgcccgcg aggcgtactc tccgtacgtc gagcggcgcg gccgagggcg
     6301 tcaacgaagc agatgggccc ttctcggccc ccggccccta ggtgcggttg gcgaggacga
     6361 gggtgcccgc ggcggatagc atcccgccca tgccgttgac gagcgagcac gaggcgccgg
     6421 ggacctggcg cgcgccggtc tcgccgcgga gctggcgggt ggcctcgatg atcgggaaga
     6481 tgccgtacat gccggagtgg gtgtaggaga gtccgccgcc gttggtgttg atgggcaggc
     6541 tgccgcccgg ggtggagcgg ccctcgctga agaactgcac gccctcgccg ggcttggcca
     6601 ggccgagcga ctccagcatg atcggcggcc ccgacgtgaa ggcgtcgtag agcatgatgt
     6661 ggttgaagtc cttgcccgag acgccggcca tcgagaaggc gcgctcgccg gccagacggg
     6721 tcgccgcgct caccgacagg ctgtccatct gggtgaccat cacgtgctcg gtggcctcgc
     6781 cgaagccgcg cacgtagacc ggggccttgg cgcagtcctt cgcccgatcg gcgcgggtca
     6841 gcacgaccgc gccgcccgcg tcggtcacga ggcagatgtt gagcaggttg aacgggtagg
     6901 acaccgggcg cgagttgagc acgtcggcca ccgtgatcgg atcgcggaac cgggccttgg
     6961 ggttcagcgc ggcccacttc cgggtcgaca ccgccacctg ggcccactgc tcgagcgtgg
     7021 tgccgtagtc gtgcatgtgc cgcgtggtga tcatgccgaa gtagctggtg gctccgccga
     7081 agccgtaggg gaactcgaac tggccgctca gcgcggtgtc gcggctgcgg gtgaccccgg
     7141 tgccggagcg gccgctctcg ccgtgcgaga ccaccgccac gtcgcagagc ccgtggtgca
     7201 gcgcggccac cgcgtgcccc accatgatga tgaacgagca cccgcccacc gtggtgccgt
     7261 ccacgtagcg cggcttgacc ccgagggcct cgcccagggt ggcgggggag tgctggcccg
     7321 cggtgaagat gccgtccacg tccttgacct ggaggcccgc gtcgcggacc gcgttcgaga
     7381 tcgcctccag gtgcagcgag agctggctca cgttgggcag cgtgccgatc tgatcgctct
     7441 cgtccacgcc cacgatgcag gcgacgttgc gcaattcccg ctgccggtcg gacacggtgg
     7501 ccatcgcgct caccgcctcg gctggaacag cggcagggtc acgtcgtcgc tcaccttggt
     7561 gaacacgacc tccaccggca tgtcgcactt gatcgccttc ggcgagggct ccacgttgat
     7621 gaggttcgac atcatgcgcg ggccctcctc gagctgcacc atcgccagca cgtagggcgg
     7681 cttgatcttc caggccttct ggaaggcctg gtgtgcgatc tcgaaggagt gcagcgtgcc
     7741 gcgcccgctc gcctgcatcc agccgatgcg acgggagtgg cagcgcgggc acaggatgcg
     7801 gggatagaag aagacgtgcc cgcagtcctc gcagcggggg agcatcagct tctcggcgcg
     7861 gcagccgtcc cagaagggct tggcctccgg ggtgatcggg gtggggagcg ggcgatcgac
     7921 gtccatgtct ctcctctctc gggagtcgcg tgacgaacga gacggagaga gactctacgc
     7981 gggggcggcg ggggtgtcaa cgaagcgggc ggcggcgcgc cccgcggggt gcgccgccgc
     8041 ccgggtactg cggctggcct gctccttccc ggctactcct tgctgatgcc ggtcggcccg
     8101 ttgctgaact cggccccgct cggcatgcgg cgcggggtgg gatcgctggc cgcccctccg
     8161 atcgggccgt tgctgaactc ggcgcccgaa ggcatgtggt ccttggtgcc gagggtggcc
     8221 ccaccgcccg ccggcccgtt gctgaactcg gcgcccgccg gcatcttggc ctcctcgacc
     8281 tcggtggtcg gggtgggatt ggtcagctcg gccgcgctca ccgtggtggc gaccgcgccc
     8341 atcgagcaaa cggcgatgac cgcaaagatc gtgctgcgct tcatgatggt tctcctcgct
     8401 gccatcgcgc ggaatgcgcc cgacggcgtt caggtcacga ctgagcttcg gactcggtca
     8461 gacggagcgg gcgaggagga cgggcggagc gcgcgtgcgg gaagcggagc ccggatcgga
     8521 ggacgccgag ccggtgccga gctcggggat caggtgcggc gtaccgcgtg gagcgggcgg
     8581 gatcgccagg gcgctgacga gcaccccgtc ggcggcgagc aggagctggt cgtccaggtc
     8641 caggttgtcg gccgagctga cgagccagcg gggatcggcg gtgcaggtgt gcgcgagggg
     8701 gctcaggagg cccagcagtc ccaccgccag aactgccacc cagaacggga acggctggcc
     8761 gcgcctcatc gagacaaggg taccttaagg cagcgtgaag gcaaccacgt agtctccgag
     8821 cttggtgccg agcttgccgt gcccgccggc cgcgatcacg atgtactgcc gcccgtcgac
     8881 gcggtacgtc atcggagtgg cctgcccgcc ggcgggcagc tcgtacttcc agagctcgcg
     8941 cccggtgtcc gaatcgaacg cccgcaggtg attgtccatc gacgccgcgg tgaacacgag
     9001 gcctccggcg gtggcgatgg ggccgccgag gttcgggctg ccgctgtcga gcagcgcggg
     9061 tcggaagagc cgcacgagca gccgcttgaa gtcgcctgat atgaccgagc cgagcggcac
     9121 gtcccaggcc gtgcggccgg agaagaggtc gaccgcgacc agcgtccccc acggcggcgg
     9181 gttgcagggc gtgccgccgg gcgacaggag cggggcgcgc cgcatcgcgt aaggggtgcc
     9241 ggcctgcagc gccagctcgg cgcggagacg ctccgcggac gtgccctgcg tggcctcgag
     9301 cgcgaggcgc tcgtgcggga tgagccgcac gagcgtcgcg agccggttcg tgttcatgaa
     9361 cagcaggtgg cgggtcggat cgtaggccga gctgccccag ttcaccccgc ccacgttgcc
     9421 cgggaacacc acggtgcccc gcaggctcgg cggggtgaag atgccctccg agcgggccgc
     9481 tgcgatcttc tcgcggcacc acgcgcggtc cttctcggtc gagccccacg cgtcctcggc
     9541 gcggaggctc tcggggacga ccgagatgcc ggaggagggc tgcgtcggcc acgcctcctc
     9601 gcccggcacg tcgctcggcg gcgccggacg ctcctcgacc gggagcagcg gcgcgccggt
     9661 gacccggtcg aggacgaaca cgcggcccat cttggtggtg atggcgatcg cgggggtgcc
     9721 gtcgcgccag gagaacagcg tgggctggga ggcgacgtcg tagtcccaca gatcgtggtg
     9781 gacgacctgg aagccccata cgagctcgcc ggtggcggcg cggagggcca cgaccgagtt
     9841 cgcccaccgg ttgtcgccct tgcgcgtgcc gccccagaag tcggggctcg cgctgccggt
     9901 cgggacgaag acgagcccgc gctccgcgtc gacggagagg gtcgaccagg cattgccggc
     9961 tccggtgcgc ggcgtggtcc ggctcgccca cgggatcggg tcccaggtcc agcggagcgc
    10021 gccggtgcgg gcgtcgaagc cccgcacgat cccccgctcc acgtcgacgg cccggttgtc
    10081 gccgatggac gacccgacga cgatcacgtc accgctgacc gcgggggctg aggtcatctg
    10141 gtagttgccg aggtcacgca ggtcgacgcc ccgggtgaga tcgacctggc cgctcgcgcc
    10201 gaagccggcg cagggcgtgc cggtctcggc gtccagcgcg atgagccggg cgtcgagcgt
    10261 gcccatgagg atgcgggtgc ggcacggcgc gccgggggag gccgcggtgt cccgccaggc
    10321 cgccaccccg cgcgaggtca cctccgagta gtcgtgcgag cgatcgagcg cggcgtcgta
    10381 ctcccagagc cgggcgccag tccgcgggtc gagggcgatg acgtggttgt agggcgtgct
    10441 gaggaacagc ttgccgtcga ccaggatcgg cgtcgcctcg aaggcggcct tgcgatcgag
    10501 cggcgcgctg tggccgaggg ctccggtgcg aaaggtccag gcgacctgga gctgggcgac
    10561 gttggtccgg tcgatctggg acgcggggga gtagcgggcg ccgcccgcgt cgttgccgta
    10621 gtgcggccac tcggagccgc cgtcgggctg cgcgctcgcg agcgacgcga gcgttaggac
    10681 cgcgaccgca agcctcaacc tcacgggcgt gaccggccgt cgcgggctag gcgggcgtgg
    10741 ggcgcgggct cgcgatggtg atcgcgtcgt ccatcaggtc gttgcccagg tcggcggcgt
    10801 cggtcggcgg caaggcatcg agatcgacgg tcggtgatga gacggccatc gctcccgccg
    10861 cgcagaacgc gaagatcgag caggtcgaat agatcatcca gtggttcatt gcggatctcc
    10921 tcatgccacc gtcgcgggga gcgcgcggcg gccgttcggg gcaccatctc gtccagggaa
    10981 cggtcagacg ggtggagcga acgagggcgg aggagcgcgg gtgagggtcg aggggcggtc
    11041 ggccgtgccc gtcgggcaat cacgggcttc gacgagcgtc tccgggacgc gcggcaccgg
    11101 gcggtggatc gcgggccggc ccgtatcgag gccgctgtac gtgacgagga acgagacgac
    11161 gtcgtcgaag tcggcgccgt cgtagaaccc ggcgatccag agaggatcgt ccggactggc
    11221 ctgggccagg ggccggagca cgacgatcag gaccagcgtc aggagcgcga cccagaaccg
    11281 gtccgggggc gggcgtctca tcgcgcggaa gcgtaccacc gcgggcgcgc cgtggatagc
    11341 cgccgtgctg cggaccggtg ggagggcctc acaccgccgg ctcgcccgcg gtcaccgcga
    11401 tgcactggcc cgacaggttc tgggccgcgt cgctgcagag gtacaccgcg agggtcgcga
    11461 tgtcctccgc ggtcgacatg cgcttgagcg gtgcccgatc gacgaaggcg cggcgcatgt
    11521 cctcgagcgc cacgccccgc acctccgcct ggccggcgat gacgcggtcg atccggtcgc
    11581 ccgcgaccgc cccgggggcg atggcgttga ccctgatgtt gtgcggggcc agctcgaggg
    11641 ccaggccgta ggtcatgccg acctgcccgg ccttcgccga gcagtagccg atgcggaagg
    11701 ggagcccccg ccggcccgcg gtggaggaga tgttgacgat ggccccgccg cccgccttga
    11761 gcatctccgg caccgcgaag cggatgcaca tgtaggagct cgtcaggcac gagttgatgg
    11821 tgtagaacca gtcgtcgctg gtgtagtcct gcacgcgctt ggtcgggccg ccgtcgcccg
    11881 cgttgttgat caggacgtcg agcttgccga aggcgcggac ccccgcctcg atcatccgct
    11941 gcacgtccgc ctcggtggcc gcgtcgccgg tgaccgcgat ggcccggcct cctgccgtct
    12001 tgaccagccc cgccgtctcc tccaccaggt cacgagttcg cgccgcgcag atgacggcgg
    12061 ccccctcctg ggcgaacagc cggctcatca cgcggccgat gcccttgctg gcgccggtga
    12121 tgagcgccac cttgccctcg agctgtccca tgcgtcctcc ttgtccctcg gtcggcgagc
    12181 ggacgggcaa ggatgtccca tcgccgctcc agtggcaaga cggtaattct tacgtcggcc
    12241 ggcgtgtacc tttttccgcc gccggcccgc ttccctcgtc gcggggatct cgccgcgtgc
    12301 gtcgacgggg agttgcgtgt cgccgccgcg cacgcgccgt ggcacgcagg ttgccactat
    12361 ccgcactagg aggtgcacca tgaccaaaaa atcgacgaag acgagaatcc tctccgccgt
    12421 ggttgggctg tccctggtct cgctcgccgc gacggcgtgc gggaccctgg gcggcgcggc
    12481 ggtcggcgcg ggcgcgggcg ccgcggtcgg cgccggaacc ggctacggag ccggcaaggg
    12541 cgcgctcatc ggtacgggtg tcggcgcggc tgccggcgcc atctacgaca tcaccaagca
    12601 ctagggtcgg gaggaattca tgacgccgta caaggcagtg acactgggaa tcatgctggc
    12661 cgtcccgctg tgggcggggt tcgcggcagg acagaccaag caatcctgcg aacagatgaa
    12721 ggccggcgcg cgccagcagg tcgagggcaa ggtggcgcgg gtcgaccaga cgcagggcgt
    12781 ggtggcggtg accgagaagg acggcacgac gcacgagttc aaggcctcga aggagacgct
    12841 ccaggatctc aaggtgggtg acaacatcga ggcgaggctg cgcgagctgc cgaagtgctg
    12901 atcgtccgct gaatcgcggc ggggccggtc gtctcccggc cccgccgccc cccgcggccc
    12961 gccccgaggt tgccgctccc gggtgggttg cgctacgatc gcccgcatga acatcctcaa
    13021 gagcctggcg gtcaaggaca tcgatgaggc cacgcgggcc atcccggtca tcgacttctc
    13081 tcccgcgttc cggtctccgg ccgatcccga agggctggag gcggtggccc gcgaggtc
//
LOCUS       DATMCV010000815         8634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_242851, whole genome shotgun sequence.
ACCESSION   DATMCV010000815 DATMCV010000000
VERSION     DATMCV010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242851"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VNU03_18770"
     CDS             <1..704
                     /locus_tag="VNU03_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWP76571.1"
                     /translation="RHTETALAAAIQVLRESVIRGDEVIYRNRPLTSEDLKKTVNVSL
                     MENDCIAEHTIIACGEQGVDPHNQGSGPIRPNQGIIFDIFPRSSRTRYYADMTRTVVK
                     GKASDELRRLYDAVLASQLRGIELIHDGASGSAIHAEVNRTLESRGYKTGVVNGRNQG
                     FFHGTGHGVGLDIHEMPRISRLDSELKTGHVVTVEPGLYYPGRGAVRIEDMVLVEGSG
                     CRNLTQSPKMELLEL"
     gene            994..2769
                     /locus_tag="VNU03_18775"
     CDS             994..2769
                     /locus_tag="VNU03_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HWP76572.1"
                     /translation="MQVTGEQFTSEAAWAGNDISTLPNFPAEIRAPAGTLFGVSSFQL
                     QFGSQRVYTPGDQLDALVVMNPAAFKVHLADLKPGGILIVNPGAFDRRNLDKAGYTAN
                     PLDDATLGEKYRLHQVDMEGLTKKALEDMPLNAKEKDRCKNFFALGLVSWVYTRPLEP
                     TLDAIKKRFAKRPEFVEANIRALKAGHAFGETAEMFAEHYGVEPAEMPPGVYRSMTGN
                     RALAWGLLAAAERTKIPVVFGAYPITPASDILHELALHKRFRVRTFQAEDEIAAVTAA
                     IGASFGGCIGVTASSGPGIALKAEAIGLAVTAELPLVIFDIQRGGPSTGLPTKTEQAD
                     LMQALYGRNSESPVVVIAPATPGDCFYIAYEAVRIAIKYMVPVMVLSDGYLANGSEPW
                     LIPDIKTLPEISVAFRTEAEGFFPYLRDPATLSRPWVRPGTPGLEHRIGGIEKQDVTG
                     NISYDPDNHDHMVRTRAEKVRRVGQEIPPLSINGPATGEVLVVGWGGTYGAITAAVEE
                     AQAEGKAVASVHLRHLNPLPPDLGHILRQYRRVLVPEINSGQLVRILRAEYLVDAVGF
                     NRVRGLPLASQEIFEAINQLLEATR"
     gene            2766..3797
                     /locus_tag="VNU03_18780"
     CDS             2766..3797
                     /locus_tag="VNU03_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HWP76573.1"
                     /translation="MSPVTGAPGATPKYTKKDFESDQDVRWCPGCGDYAILSAVQKMM
                     PDLGIPREDIVFISGIGCSSRFPYYMNTYGFHTIHGRAPAVATGLKLARPELKIFVVT
                     GDGDGLSIGGNHLLHVLRRNVDLTILMFNNRIYGLTKGQYSPTSELGKVTKSTPVGSA
                     DRPVSPCAFALAAGATFVARTVDRNIAHMEETLKRAAAHKGAAFVEILQNCNVYNDMA
                     WSVLYDRESKVLHELRLQHGKPLVWGPLDDRRGLVLEGVKPKVVKVADVAESALWVHD
                     ETDGGTALVLAQLWAPEYPIPLGVLANKAGESTYEEILIQQEQKVISERGPGDIAKLL
                     MSGDTWKIG"
     gene            3880..5325
                     /locus_tag="VNU03_18785"
     CDS             3880..5325
                     /locus_tag="VNU03_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWP76574.1"
                     /translation="MPLPPPVLRELERIVGAAGLIVSREGLLTYECDMHTFYKGAPEA
                     VVLPESAGQVAEIVRLCRRERVPIVPRGSGTGLIGGAMAPEGGVMVSMTRMNRIVEID
                     FANRCATVQPGLINLWLSQATREGGYFFAPDPSSQMVSSIGGNASTNAGGPHCLKYGI
                     TVNHILGLEVVTGAGELVRLGGKTHERPGYDLTGVMVGAEGTLGLITGVVVRLTHLPE
                     SVKTILASFSSIEDASETVSAIIRAGIIPAALEMLDEVVIRAIEEGIHAGYPKGAGAV
                     LLIELDGPVAEIEAQAAPIEAICREHLALEIRVARSEAERALLWKGRKEAAGVFGRLT
                     PNWLLQDAVVPRSKLPEIMRELQAIGARHRVVIANVFHAGDGNLHPLICYDDRVPGEL
                     ERAKHANEDLLHACLRLGGSVTGEHGVGLDKCESLPLQFAEADLNFMARLRRAFDPDS
                     IMNPGKLLPSHPACGEGFRPTARPLPAGTWI"
     gene            5328..6638
                     /locus_tag="VNU03_18790"
     CDS             5328..6638
                     /locus_tag="VNU03_18790"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP76575.1"
                     /translation="MAVSTPALHDALAELVGPAHVLRDPVARARHAVDGLVPGVVVRP
                     GGPEEVSRVLALCSSERLAVVARGAGTSQGLGNPPRRLDLVLELGRLAAVREYVPEDM
                     VATVETGLTLAGLAAHLAPHRQMLALDPPGGDARSVGGVLATHASGALRFRYGTGRDL
                     LLGARFVQADGTVTWGGAKVVKSVTGYDVPKLLVGSLGTLAILVEATLRLHPMPPATR
                     SWQVGLPSYAGARAFLAALLDSPLQPDRAALLDAAGLRRAGLTPDPIALLLSIGSVAE
                     AVEQQGQALEGLASGHGGRIEGIADSAWSALGAAVDAPIRLRLAGEPRRLLDWVAEGQ
                     AGADRLGAETALLAQPGHGVIQMAVWGSQEPRRVGAELVRPLRAAVEAEGGSLVLERA
                     PVELKIDCDAWGSISPKILTIMKRIKTEFDPQDCLSPGRFVGGL"
     gene            6692..7927
                     /locus_tag="VNU03_18795"
     CDS             6692..7927
                     /locus_tag="VNU03_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWP76576.1"
                     /translation="MDGLRACVHCGICLPQCPTYRVLGEEMDSPRGRVYLMRAAAEDR
                     IALTPTMARHLDLCLGCRACETACPSGVPFGQLLEATRGQFERRGVTAPQRDSATLRF
                     LLEVFPHPGRLGPMLHALRMYQWSGMQALVRGLGLLAPFAKLRAMEALLPPVPAPAAP
                     LPEVVPARGGRARGRAGVLLGCAQRFFYPDVNADTVRLLSAAGYEVVVPRQQECCGAL
                     HLHAGRIDDFRAMARRLMPAFDDVDVVVVNAAGCGSALKEYGHWLPEDAARLFALRVR
                     DVSEVLADADLPLRPLPLTVTYHDACHLVHGQRVRAEPRSLLRRIPELTLVDLPDGEL
                     CCGSAGVYNLLEPEMAGELGRRKAARIRETGARVVAAGNPGCLMQIRQHCRALGLEIE
                     VVHPVTLLARALVEKETAR"
     gene            7924..8265
                     /locus_tag="VNU03_18800"
     CDS             7924..8265
                     /locus_tag="VNU03_18800"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP76577.1"
                     /translation="MKIRVRDHVKVAADRMAKIALSGTARTQLDLYCVAPGQSQSPHR
                     HEGEDKIYYVIEGSGRFTVGDSEERLEAGDALVAPAGVGHGLVNDGSALLLVLVLVAP
                     PPAHVKRGPAS"
     gene            8270..>8634
                     /locus_tag="VNU03_18805"
     CDS             8270..>8634
                     /locus_tag="VNU03_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWP76578.1"
                     /translation="MATPVRAALMITCLGDMFFPEVGVAMVRLLRGLGVEVEFPEGQT
                     CCGMPLFNSGYHHDAALVAARTVKLFERAEHVVVPSGSCAWMVKTEYPGLLKHDPALR
                     AAAESLARRTRELSQFLVEV"
BASE COUNT         1252 a         3043 c         3106 g         1233 t
ORIGIN      
        1 agcggcacac cgagaccgcg ctcgcggcgg cgatccaggt gctccgggag agcgtgatcc
       61 gcggggacga ggtgatctac cggaaccgcc cgctgacctc ggaggacctc aagaagacgg
      121 tgaacgtgtc gctgatggag aacgactgca tcgccgagca caccatcatc gcctgcgggg
      181 agcagggggt cgacccgcac aaccaggggt cggggcccat ccgcccgaac cagggaatca
      241 tcttcgacat cttcccgagg tcgtcccgga cccgctacta cgcggacatg acccgcacgg
      301 tggtcaaggg caaggcctcc gacgagctcc ggcgcctcta cgacgcggtg ctcgccagcc
      361 agctccgggg catcgagctg atccacgacg gggcctccgg ctcggccatc cacgccgagg
      421 tcaaccgcac cctggagagc cggggctaca agaccggggt ggtgaacggt cggaaccagg
      481 gcttcttcca cggaaccggc cacggggtcg gcctcgacat ccacgagatg ccgcggatca
      541 gccgcctgga ctccgagttg aagaccgggc acgtggtgac cgtcgaaccg gggctgtact
      601 accccggccg cggggcggtc cggatcgagg acatggtgct ggtggagggg agcggctgcc
      661 ggaacctgac ccagtccccc aagatggagc tgcttgagct ctagcggcgg cttccgggag
      721 ggggcggtcg ggcggtcaga ccgcggacaa ccttgcttcc attcagacgt ttgggtagca
      781 tgactaggga gtgtcacgcc tctgatcgtt gctttccaga agccagggtg accgagccac
      841 tccgcctgca ggctgccgtc gaacggggca tgcgtttgga cggaaaggac accgaagatg
      901 agtgagagtg tagccgccac gccgacggct tcgaagccgc gccgcaccct gacgcgcgcc
      961 gtgatccggt tcgccggaga ctcgggcgac ggcatgcagg tgaccggcga gcagttcacg
     1021 agcgaggccg cgtgggccgg caacgacatc tccacgctgc ccaatttccc ggccgagatc
     1081 cgggcccccg ccggcaccct cttcggggtg tcgagcttcc agctccagtt cggcagccag
     1141 cgggtctaca ccccgggcga tcagctcgac gcgctggtgg tgatgaaccc ggccgcgttc
     1201 aaggtgcacc tggccgatct caagccgggc ggcatcctga tcgtgaatcc cggcgccttc
     1261 gacaggcgga acctcgacaa ggcgggttac accgccaacc cgctcgacga cgcgacgctg
     1321 ggcgagaagt accgcctgca ccaggtcgac atggaggggc tcaccaagaa ggccctcgag
     1381 gacatgccgc tgaacgcgaa ggagaaggat cgctgcaaga acttcttcgc gctcggtctc
     1441 gtgtcctggg tgtacacccg gccgctcgag cccaccctgg acgcgatcaa gaagcggttc
     1501 gccaagcgcc ccgagttcgt ggaggccaac atccgcgcgc tgaaggcggg gcacgccttc
     1561 ggcgagaccg cggagatgtt cgccgagcac tacggcgtcg agccggccga gatgccgccc
     1621 ggcgtgtacc ggagcatgac cggcaatcgc gcgctggcct ggggcctgct cgcggcggcc
     1681 gagcggacga agatcccggt ggtgttcggg gcctacccga tcacgcccgc gagcgacatc
     1741 ctgcacgagc tggcgctcca caagcgcttc cgggtgcgca ctttccaggc cgaggacgag
     1801 atcgcggcgg tcaccgcggc gatcggggcc tcgttcgggg gctgcatcgg ggtgaccgcg
     1861 tcgagcggcc ccggcatcgc gctcaaggcg gaggccatcg ggctcgcggt caccgccgaa
     1921 ctcccgctcg tgatcttcga catccagcgt ggcgggccga gcaccggcct gcccaccaag
     1981 accgagcagg ccgacctgat gcaggccctg tacggccgca acagcgagtc gccggtggtg
     2041 gtgatcgccc ccgcgacccc gggcgattgc ttctacatcg cgtacgaggc ggtgcggatc
     2101 gccatcaagt acatggtgcc ggtgatggtg ctctccgacg ggtacctggc caacggctcc
     2161 gagccgtggc tgatccccga catcaagacg ctgcccgaga tctcggtcgc cttccggacc
     2221 gaggccgagg ggttctttcc gtacctgcgg gatccggcca ccctctcgcg gccctgggtg
     2281 cgcccgggga cgccggggct cgagcaccgc atcggcggca tcgagaagca ggacgtgacc
     2341 ggcaacatct cttacgaccc cgacaaccac gaccacatgg tccgcacccg cgccgagaag
     2401 gtgcggcggg tgggccagga gattccgccg ctctcgatca acggcccggc caccggcgag
     2461 gtgctggtgg tgggctgggg cggcacctac ggggccatca ccgccgcggt cgaggaggcg
     2521 caggccgagg gcaaggcggt cgcctcggtg cacctgcgcc atctcaaccc gctgcccccg
     2581 gacctcggcc acatcctccg ccagtaccgg cgggtgctgg tgcccgagat caacagcggg
     2641 cagctcgtgc gaattctccg ggcggagtac ctggtcgacg cggtgggctt caaccgcgtg
     2701 cgcggcctgc cgctcgcgag ccaggaaatc ttcgaagcca tcaaccaatt gctggaggcc
     2761 acacggtgag tcccgtaacc ggagcgcccg gagcgacccc gaagtacacc aagaaggatt
     2821 tcgagtccga ccaggacgtg cgctggtgcc ccggctgcgg ggactacgcg atcctgagcg
     2881 cggtgcagaa gatgatgccc gacctcggca tcccgaggga ggacatcgtg ttcatctccg
     2941 ggatcggctg ctcgagccgc ttcccgtact acatgaacac gtacggattc cacaccatcc
     3001 acggccgggc tcccgcggtc gccaccggcc tcaagctggc gcggcccgag ctcaagatct
     3061 tcgtcgtcac cggtgacggc gacggcctca gcatcggcgg taatcatctg ctgcacgtgc
     3121 tgcggcgcaa cgtggacctg accatcctga tgttcaacaa ccggatctac ggcctcacca
     3181 agggccagta ctcgccgacc agcgagctgg gcaaggtgac caagtccacc ccggtgggct
     3241 cggccgaccg gccggtgagc ccgtgcgcgt tcgcgctcgc ggccggggcc accttcgtgg
     3301 cgcggacggt ggaccgcaac atcgcccaca tggaggagac gctcaagcgg gccgcggccc
     3361 acaagggcgc cgccttcgtc gagatcctcc agaactgcaa cgtgtacaac gacatggcgt
     3421 ggagcgtgct ctacgaccgc gagtccaagg tgctccacga gctgcgcctg cagcacggca
     3481 agccgctcgt gtgggggccc ctcgacgacc gccgcggcct cgtgctggaa ggcgtcaagc
     3541 ccaaggtcgt gaaggtggcc gacgtcgcgg agagcgcact ctgggtgcac gacgagaccg
     3601 acggggggac cgcgctggtg ctggcgcagc tgtgggcgcc cgagtacccg attccgctcg
     3661 gggtgctcgc caacaaggcg ggcgagtcca cctacgagga gatcctgatc cagcaggagc
     3721 agaaggtcat ctcggagcgc ggccccggcg acatcgccaa gctgctcatg tcgggcgata
     3781 cctggaagat cggctgaacc cccgcgtccg gcgggcagcc cgtagatcac cggccctccc
     3841 tgggagggcc ggtggtattt tgtcagcctg agacgcccca tgcccctgcc tcccccggtc
     3901 cttcgcgagc tcgagcgcat cgtcggcgcc gccgggctga tcgtctcccg cgagggcctc
     3961 ctcacctacg agtgcgacat gcacacgttc tacaagggtg cgccggaggc ggtggtgctg
     4021 cccgagtcgg ccgggcaggt ggccgagatc gtccgcctct gccgccgcga gcgggtgccg
     4081 atcgtgccgc ggggatcggg caccgggctc atcggcggcg ccatggcccc cgagggtggg
     4141 gtgatggtct cgatgacccg catgaaccgg atcgtggaga tcgacttcgc gaatcgctgc
     4201 gccaccgtgc agcccgggct catcaacctg tggctgagcc aggccacccg cgagggcggc
     4261 tacttcttcg cgccggatcc ctcgagccag atggtgtcgt ccatcggtgg gaacgcgtcc
     4321 acgaatgcgg gcgggcccca ctgcctcaag tacgggatca ccgtgaacca cattctcggc
     4381 ctcgaggtcg tcaccggggc gggggagctg gtgcggctcg ggggcaagac ccacgagcgc
     4441 cccggctacg atctcaccgg ggtcatggtc ggggccgagg gcaccctcgg cctcatcacc
     4501 ggcgtggtgg tccggctcac ccacctcccg gagtcggtga agacgatcct cgcgtccttc
     4561 tcgagcatcg aggacgcctc cgagacggtg tcggcgatca tccgggccgg catcatcccg
     4621 gccgcgctcg agatgctgga cgaggtggtg atccgcgcca tcgaggaggg catccacgcc
     4681 ggctatccca agggcgcggg cgcggtgctc ctgatcgagc tggacggccc cgtcgccgag
     4741 atcgaggccc aggccgcgcc gatcgaggcg atctgccgcg agcacctggc gctggagatc
     4801 cgggtggcgc ggagcgaggc ggagcgcgcg ctcctctgga agggccggaa ggaggcggcg
     4861 ggcgtcttcg ggcgcctcac ccccaactgg ctgctccagg acgcggtggt gccccgctcg
     4921 aagctgccgg agatcatgcg cgagctgcag gccatcgggg cgcggcaccg ggtggtgatc
     4981 gccaacgtgt tccacgccgg cgacggcaac ctccacccgc tcatctgcta cgacgaccgg
     5041 gttcccggcg agctcgagcg cgccaagcac gccaacgagg atctgctcca cgcgtgcctg
     5101 cgcctcggcg gcagcgtgac cggcgagcac ggggtcgggc tcgacaagtg cgagagcctg
     5161 ccgctgcagt tcgcggaggc ggacctgaac ttcatggccc ggctgcgacg cgccttcgat
     5221 ccggactcga tcatgaatcc gggcaagctc ctgccgtcgc acccggcgtg tggcgagggg
     5281 ttccgtccca ccgctcgccc gctgcccgcc ggcacctgga tctgacggtg gcggtctcga
     5341 cgcccgccct tcacgacgcg ctcgccgagc tggtcggccc cgcccacgtg ctccgcgatc
     5401 cggtggcgcg ggcgcgccac gcggtggacg gcctcgtgcc cggcgtggtg gtccggcccg
     5461 gagggccgga agaggtgagc cgcgtgctgg ccctctgctc gagcgagcgg ctcgcggtcg
     5521 tcgcccgcgg cgccggcacc agccagggcc tgggcaaccc gccgcggcgg ctcgacctcg
     5581 tgctggagct gggacgtctc gccgcggtcc gggagtacgt gcccgaggac atggtggcga
     5641 cggtcgagac cggcctcacc ctcgccggtc tggccgcgca cctggctccg caccggcaga
     5701 tgctggcgct cgatccgccg ggaggcgacg cgcgcagcgt gggcggcgtc ctggccaccc
     5761 acgcgagcgg cgcgctccgc ttccgctacg gcaccggacg cgatctgctg ctgggcgcgc
     5821 gcttcgtcca ggccgacggc acggtcacct gggggggcgc caaggtggtg aagtcggtca
     5881 ccggatacga cgtgcccaag ctgctggtcg gctcgctcgg cacgctcgcg atcctcgtgg
     5941 aggcgacgct ccggctgcac ccgatgcccc cggccacccg ctcctggcag gtcggcctgc
     6001 cctcgtacgc gggcgcacgc gcgttcctgg ccgccctgct ggattcgcca ctgcagcccg
     6061 atcgggccgc cctgctcgac gccgccggct tgcgccgggc cgggctgacc ccggacccga
     6121 tcgcgctgct gctctcgatc ggcagcgtgg ccgaggcggt ggagcagcag ggccaggcgc
     6181 tcgaggggct cgcgtccggt cacggggggc gcatcgaggg tatcgccgac tcggcctggt
     6241 cggcgctcgg cgcggcggtg gacgccccga tccgactgcg gctggccggc gagccccggc
     6301 gcctgctcga ctgggtcgcg gagggacagg ccggggccga ccgcctggga gccgagaccg
     6361 cgctcctggc ccagcccgga cacggcgtga tacagatggc ggtctggggg agccaggagc
     6421 cgcgacgggt gggcgccgag ctcgtgcggc ccctgcgggc cgcggtcgag gccgaggggg
     6481 gcagcctggt cctggagcgg gccccggtcg agcttaagat cgactgtgat gcttggggtt
     6541 ctatcagtcc taaaatattg acgatcatga aacggatcaa gaccgaattc gacccccagg
     6601 attgcctgag cccggggcgc ttcgtggggg gcctgtgagc cagggcaccc cggtcaggct
     6661 cggcgccttc gatgcccagg acgcgccgtc catggacggg ctgcgggcgt gcgtccactg
     6721 cgggatctgc ctgccgcagt gtccgaccta tcgcgtgctg ggcgaggaga tggactcgcc
     6781 ccgcggccgg gtctacctca tgcgcgcggc cgccgaggat cggatcgcgc tcactccgac
     6841 catggcgcgg cacctcgacc tctgtctcgg ctgtcgcgcg tgcgagaccg cgtgcccctc
     6901 cggcgtgccg ttcggccagc tgctcgaggc cacgcgcggg cagttcgagc gacgcggggt
     6961 caccgcaccc cagcgcgact cggcgaccct gcgcttcctc ctcgaggtct ttccacatcc
     7021 gggccgcctc ggccccatgc tccacgcgct ccgcatgtac cagtggtcgg gcatgcaggc
     7081 gctcgtgcgc gggctcggcc tgctggcgcc gttcgcgaag ctccgggcga tggaggcgct
     7141 cctgccgccg gtgcccgcgc cggccgctcc gcttccggag gtggtcccgg cccgcggcgg
     7201 ccgggcgcgc ggccgggccg gcgtcctgct gggttgcgcg cagcgattct tctaccccga
     7261 tgtcaacgcc gacaccgtcc ggcttctctc cgcggcgggc tacgaggtgg tggtgccgcg
     7321 gcagcaggag tgctgcgggg cgctccatct gcacgccggg cgcatcgacg acttccgggc
     7381 gatggcgcgg cggctgatgc ccgccttcga cgacgtcgac gtcgtggtgg tgaacgcggc
     7441 gggctgcggc tcggcgctca aggagtacgg gcactggctg ccggaggacg cggcgcggct
     7501 ctttgccctg cgggtgcgcg acgtctcgga ggtgctcgcg gacgccgacc tgccgctgcg
     7561 cccgcttccc ctgaccgtga cctaccacga cgcctgccac ctggtccacg ggcagcgcgt
     7621 gcgcgccgag ccccgcagcc tgctgcgccg catcccggag ctgaccctgg tggatctgcc
     7681 cgacggcgag ctctgctgcg ggagcgcggg tgtctacaac ctgctcgagc ccgagatggc
     7741 gggggagctc ggccggcgga aggcggcgcg catccgggag accggcgcgc gcgtggtggc
     7801 ggcgggcaac ccgggctgcc tgatgcagat ccgccagcac tgccgcgcgc tgggcctcga
     7861 gatcgaggtc gtgcacccgg tgacgctgct ggcccgggcc ctcgtggaga aggagaccgc
     7921 gcgatgaaga tccgggtacg ggaccacgtg aaggtggcgg ccgaccggat ggccaagatc
     7981 gccctgtccg gaaccgcgcg cacccagctc gacctctact gcgtggcgcc cggccagtcg
     8041 cagtcgcccc accgccacga gggcgaggac aagatctact acgtcatcga ggggagcggg
     8101 cgcttcacgg tgggcgactc ggaggagcgg ctcgaggcgg gcgacgccct ggtggccccg
     8161 gccggagtgg gccacggcct ggtcaacgac ggctccgcgc tcctgctggt gctggtgctc
     8221 gtggcgccgc cgccggccca cgtcaagcgc gggcccgcga gctagcccgg tggccacccc
     8281 ggttcgcgcc gcgctcatga tcacctgcct gggcgacatg ttcttcccgg aggtgggcgt
     8341 ggcgatggtg cggctgctcc ggggcctggg cgtggaggtc gagttcccgg agggccagac
     8401 ctgctgcggc atgccgctgt tcaactcggg ctatcaccac gacgcggcgc tggtggcggc
     8461 ccggaccgtg aagctcttcg agcgcgcgga gcacgtggtg gtgccctcgg gctcctgcgc
     8521 gtggatggtg aagaccgagt acccgggcct gctcaagcac gatccggccc tgcgcgccgc
     8581 cgccgaatcg ctcgcccgcc gcacgcgcga gctctcccag ttcctcgtgg aggt
//
LOCUS       DATMCV010000816         2552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_707876, whole genome shotgun sequence.
ACCESSION   DATMCV010000816 DATMCV010000000
VERSION     DATMCV010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707876"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1010
                     /locus_tag="VNU03_18810"
     CDS             141..1010
                     /locus_tag="VNU03_18810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP76579.1"
                     /translation="MHTLALGRFRVDAVVETAGPTRPTWLFPAATPEALAPHLGWLAP
                     HFVDAEGRLRQSVHTFVIRSSDLTVLVDTCIGNDKDRGGRRPFHMLRTGFLEDLRAAG
                     VAPEAVDVVICTHLHVDHVGWNTRLADGRWVPTFPRARYLFARREWEHWTSEEEDGTK
                     RILADSVAPVLDAGLAELVEMDHRVSDGIWLEPTPGHTPGHVSVRLHADGADAVITGD
                     LMHHPVQVAEPAWQSAFDTDPAQARATRRAFCARYADRPVDVLGTHFHHPTAGRIVTH
                     RDAWRFVVDEPRT"
     gene            1076..1594
                     /locus_tag="VNU03_18815"
     CDS             1076..1594
                     /locus_tag="VNU03_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWP76580.1"
                     /translation="MPTHYRGSAQERNALDAYIKLMRAADSVTARLDPLMRAADLTVG
                     QFGALEALLHRGPLCQRDLGRKLLRSGGNITVVVGNLARRGLVRRHRGPQDRRFVTVT
                     LTDKGRRVIGGIFPRHVRHVVRELGALSPPEQAELGRLCRQLGLARRPERARKERSNG
                     AQSHPLRLARAL"
     gene            1551..2066
                     /locus_tag="VNU03_18820"
     CDS             1551..2066
                     /locus_tag="VNU03_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HWP76581.1"
                     /translation="MAPNRTRFGSLAHYEKGGVEVIKDDARNYVFSNVFEVASKARPY
                     EKVAVGKNIEYVIEAIRAEGTSGWRVACQDEFALVMDGEVEVRLLKPDDASLAPAGSH
                     GSIPLAVAPAGRKMGRVRARRGHMTLLPAGAAYQFHAGSPSVILLQTLAGRDTVERWA
                     EICQTTPRPQA"
     gene            2080..>2552
                     /locus_tag="VNU03_18825"
     CDS             2080..>2552
                     /locus_tag="VNU03_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HWP76582.1"
                     /translation="MATDTIPELGSIEPNRHGYKEFALGKFSFSRDEYFAHVRWPNGT
                     HMLAADAFLRAIQRDVAWGFFYGIVNFDTVIGTVNHYGTVDLFAGRFNSQYREAGLAY
                     SENFKSDAISRLFEAILLDWTNATFDPFASPAETGSAFGPKNGSNKRAVTRHRVTA"
BASE COUNT          374 a          944 c          883 g          351 t
ORIGIN      
        1 ccggccggat ccggtcacga tcgccacctt gccgtcgagc tgtcccatgc cgtcgcgcct
       61 ccttgcgcgg atcgcggccg atcatacacg atccgctcgg ccctggtcct cgctcgccgc
      121 gttgggtaga atcctcgacc atgcacaccc tcgcgctcgg gcgcttccgc gtcgacgcgg
      181 tcgtggagac cgcgggcccg actcggccga cgtggctctt cccggccgcg actcccgagg
      241 cgctcgcgcc ccacctgggg tggctcgcgc cgcacttcgt cgacgcggaa gggcggctcc
      301 ggcagagcgt gcacaccttt gtgatccgat cctccgacct gaccgtgctc gtcgacacct
      361 gcatcggcaa cgacaaggac cgcggcggcc ggcgcccgtt ccacatgctc cgcaccgggt
      421 tcctcgagga cctgcgcgcg gcgggggtgg ctccggaggc ggtggacgtg gtgatctgca
      481 cccacctgca cgtcgatcac gtgggctgga acacccgtct cgccgacggc cgctgggtgc
      541 cgacctttcc ccgcgcccgc tacctcttcg cccggcggga gtgggagcac tggacgtccg
      601 aggaggagga cggcaccaag cgcatcctgg ccgacagtgt cgccccggtg ctcgacgcgg
      661 ggctcgccga gctggtggag atggatcacc gcgtctcgga cggcatctgg ctcgagccga
      721 cccccggcca cacccccggg cacgtgagcg tgcgcctgca cgccgacggc gccgacgcgg
      781 tgatcaccgg cgacctcatg caccacccgg tccaggtcgc ggagcccgcg tggcagagcg
      841 cgttcgacac ggatccggcc caggcgcggg cgacccggcg cgcgttctgc gcccgctacg
      901 cggaccggcc ggtcgacgtg ctcggcaccc acttccacca cccgaccgcg gggcgcatcg
      961 tgacccaccg cgacgcgtgg cgcttcgtgg tggacgagcc gcggacgtag ccgcgagcgc
     1021 gacgcccttg acaacgcggc ggcgcgggta ctaatagacg gttcaagttc aaagtatgcc
     1081 cacccactac cggggcagcg cgcaggagcg gaacgcgctc gacgcgtaca tcaagctcat
     1141 gcgcgcggcc gactcggtga ccgcgcgcct cgatccgctg atgcgcgccg ccgacctgac
     1201 cgtcggccag ttcggtgcgc tggaggcgct gctccaccgg gggccgctct gccagcggga
     1261 cctgggccgc aagctcctcc gcagcggggg aaacatcacg gtggtggtcg gcaacctcgc
     1321 gcggcgcggg ctcgtgcgcc gccaccgggg gccgcaggat cggcggttcg tcacggtcac
     1381 gctcaccgac aagggccgcc gagtgatcgg cgggatcttt cctcgccacg tgcggcacgt
     1441 cgtgcgcgag ctgggggcgc tctccccgcc ggagcaggcg gagctgggcc ggctctgccg
     1501 gcagctcggc ctcgcgcggc ggccggagcg cgcccgaaag gagaggagca atggcgccca
     1561 atcgcacccg cttcggctcg ctcgcgcact atgagaaggg cggcgtcgag gtcatcaagg
     1621 acgacgcgcg caactacgtc ttctcgaacg tgttcgaggt cgcctcgaag gctcgaccct
     1681 acgagaaggt cgcggtcggc aagaacatcg agtacgtgat cgaggccatc cgcgccgagg
     1741 gcacctcggg ctggcgggtg gcgtgccagg acgagttcgc cctggtgatg gacggggagg
     1801 tggaggtgcg gctgctcaag cccgacgacg cgtcgctcgc gccggcgggg agccacggct
     1861 cgatcccgct cgcggtcgct cccgccggac gcaagatggg gcgggtgcgg gcccggcgcg
     1921 gccacatgac gctcctaccc gccggcgcgg cctaccagtt ccacgccggg agcccgagcg
     1981 tgatcctgct ccagaccctg gccggtcgcg acacggtgga gcgctgggcc gagatctgcc
     2041 agaccacgcc gcggccccag gcctgagagg agaccgacca tggccaccga caccatcccc
     2101 gagctcggca gcatcgagcc caaccggcac ggctacaagg agttcgcgct cggcaagttc
     2161 tcgttcagcc gggacgagta cttcgcccac gtgcgctggc ccaacggcac ccacatgctc
     2221 gcggcggacg ccttcctgcg cgcgatccag cgcgacgtgg cgtggggctt cttctacggc
     2281 atcgtcaact tcgacaccgt gatcgggacg gtcaatcact acggcacggt ggatctgttc
     2341 gcgggccggt tcaactcgca gtaccgggag gccggcctcg cctactcgga gaacttcaag
     2401 agcgacgcga tcagccggct cttcgaggcg atcctgctcg actggacgaa cgcgaccttc
     2461 gaccccttcg cgtcccccgc ggagaccggc agcgccttcg ggcccaagaa cggctccaac
     2521 aagcgcgcgg tcacccgcca ccgggtcacc gc
//
LOCUS       DATMCV010000817         2111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_111029, whole genome shotgun sequence.
ACCESSION   DATMCV010000817 DATMCV010000000
VERSION     DATMCV010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111029"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="VNU03_18830"
     CDS             complement(<1..2020)
                     /locus_tag="VNU03_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76583.1"
                     /translation="MPRPARRPAAPFILGLSLAAAVAWAPAVPAAAAQVESLLPSDRL
                     TVPDPGQATGRRIALPAPDCGVDAAGCDEVRLLNELDGWSVNPRVAIRFSGPMALDSV
                     TRTTAFIVPLWAEPLPSPVGLTQLVWDAEQHTLYARPERPLLQARRYALVLTAKILDE
                     SGQPLVPLAGRGLVSEAAQGVSAQGMVFHQLKTLGVARRDLAAVSVFTTQSVTAGLEQ
                     MRAAVDLGQAPVIHFTLGPGGARSVYTRAELDGIELRRQVASDPAAALEDPEKLPLVL
                     VPPTEVRAIAFGRFSARSFLTADRHVPATATRSEAPAPQGSEDVDVTVFMPAGTMPRD
                     GWPVAIFGHGFGNERHSVPMVVAGTMARAGFATVAINVVGHGGGPAGTLTVSRAGQEP
                     VTLPAGGRGLDLNGDGRIGVTEGVATRAASPLALISMRDGLRQTTADLMQLVRAIHRG
                     VDVDGDGRVDLDRERIQYFGQSFGGIYGTLLMAVDPLVRMGVLNVAGGPIVEIGRQSP
                     VFRGLALNQLRQRQPPLANGENDFTESLPLPGEAPVRDPAPGALAIQAYLARAEWLSQ
                     SANPVAFAPYLRQAPLPGVPARPVLFQWAVGDQTVPNPTTTALLDAGLLRPVSSLYRH
                     DVLGPGLPDRFRDPHAFLTWTAFPEVYAIGRAAQEQVARFFLSGGRQIE"
BASE COUNT          287 a          745 c          847 g          232 t
ORIGIN      
        1 cctcgatctg ccggccgccg gagagaaaaa agcgcgccac ctgctcctgc gccgcgcgcc
       61 cgatggcgta cacctcgggg aacgcggtcc aggtcaggaa ggcgtgcggg tcgcggaagc
      121 gatccgggag gccggggcca agcacgtcgt gacgatagag cgaagagacc ggccggagca
      181 agcccgcgtc aaggagcgcc gtcgttgtcg ggttgggcac ggtctgatcg cccaccgccc
      241 actggaacag gacggggcgc gccggcacgc cgggcagggg cgcctgacgc aggtacgggg
      301 cgaaggcgac cgggttggcg ctctgggaca gccactccgc gcgggcgagg taggcctgga
      361 tggcgagcgc gcccggcgcc gggtcgcgca ccggggcctc gccgggcagc ggcagggact
      421 cggtgaagtc gttctccccg ttggccaggg gcggctggcg ctggcggagc tggttcaggg
      481 ccagcccgcg gaagaccggc gactgccgcc cgatctccac gatgggcccg cccgccacgt
      541 tgagcacgcc cattcgcacc agcgggtcca ccgccatcag gagcgtgccg tagatgccgc
      601 cgaacgactg gccgaagtac tggatgcgct cgcggtcgag atcgacgcgg ccgtcgccgt
      661 ccacgtcgac accgcggtgg atggcgcgga cgagctgcat gaggtcggcg gtggtctggc
      721 ggaggccgtc gcgcatgctg atcagcgcga gcggcgaggc ggcgcgggtg gcgacgccct
      781 cggtcacgcc gatgcgcccg tcgccgttca ggtccaggcc gcgcccaccc gcgggcagcg
      841 tcaccggctc ctggccggcg cgtgacacgg tgagcgtgcc ggcgggccca ccgccgtggc
      901 cgaccacgtt gatcgcgacg gtggcgaagc cggcgcgcgc catggtgccg gccaccacca
      961 tcggcaccga atggcgctcg ttgccgaagc cgtggccgaa gatcgcgacc ggccagccgt
     1021 cccgcggcat cgtgcccgcg ggcatgaaga cggtgacgtc gacgtcctcg ctcccctgag
     1081 gcgcgggcgc ctcgctgcgg gtggcggtgg cgggcacgtg gcgatcggcg gtgaggaacg
     1141 atcgcgcgct gaagcggccg aacgcgatcg cgcggacctc ggtcggcggc accaggacca
     1201 gcggcagctt ctccgggtcc tcgagcgccg cggcggggtc ggacgcgacc tggcggcgca
     1261 gctcgatccc gtcgagctcg gcgcgcgtgt agaccgagcg cgcgccgccg gggccgagcg
     1321 tgaagtggat gaccggcgct tggccgaggt cgaccgcggc gcgcatctgc tcgaggcccg
     1381 cggtcacgct ctgggtcgtg aagaccgaca ccgcggcgag gtcgcgccgc gccaccccga
     1441 gcgtcttgag ctggtggaag accatgccct gcgcggagac gccctgcgcc gcctcgctca
     1501 ccagcccgcg gccggcgagg ggcaccagcg gctggccgct ctcgtcgagg atcttcgcgg
     1561 tcagcaccag ggcgtagcgg cgcgcctgca ggagcggccg ctccgggcgg gcgtacaacg
     1621 tgtgctgctc cgcatcccac acgagctggg tgaggcccac cggcgacggc agcggctcgg
     1681 cccagagcgg cacgatgaac gcggtggtcc gcgtgacgct gtcgagcgcc atcggcccgg
     1741 agaaccggat ggcgacccgc gggttgacgc tccagccgtc cagctcgttg aggagccgca
     1801 cctcgtcgca gcccgcggcg tcgacaccgc agtcgggcgc cggcagggcg atgcggcggc
     1861 cggtcgcctg gccgggatcc ggaacggtca gccggtcaga cgggaggagc gactcgacct
     1921 gggccgcggc cgccgggacc gccggcgccc aggcgaccgc cgccgccagc gacagcccga
     1981 ggatgaaggg ggcggccggg cggcgggccg ggcgaggcac cggtctcagc gaggctgctg
     2041 gggcgacgga ggcgacggct gcggttgggg ctgccgcggc gccggcacgg gcccggggcg
     2101 gcgaggcgca c
//
LOCUS       DATMCV010000818         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_707929, whole genome shotgun sequence.
ACCESSION   DATMCV010000818 DATMCV010000000
VERSION     DATMCV010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707929"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VNU03_18835"
     CDS             <1..897
                     /locus_tag="VNU03_18835"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP76584.1"
                     /translation="LILPISGAVHAGFQLAHDRYSYLSGLGFALVAGGAIAWVLAAAG
                     ARRVGRPVLAAGLGVATLVIGALAVQSWQQLRIWRDSDTLWRWSLEADPRCAVCANNL
                     AALIINQPSPKPAQLTEAESLARRALAQRPTYDSAHNTLGVVFAQRGEDGAAETEFRQ
                     AIQLSPDRVASVANLGALYARTGRPEEALPLLRRAWAKEPDYPGLRTSLGFTLRDAGI
                     VQARAGHLPAAIGLLQEAEREIPDDADVHRNLGLALWEHGQSAAAVPYLERAVTLRPD
                     DENGRRLLTRLRTDPEHPPSFR"
     gene            894..>2155
                     /locus_tag="VNU03_18840"
     CDS             894..>2155
                     /locus_tag="VNU03_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76585.1"
                     /translation="MSHRWILPVSVTVLTVICFLPALTGSFLNWDDNVNFLENTAYRG
                     LGPAQVRWAFTSVLFGHYIPLTRLTWSLNYVLGGLDPSGYHLVNLLLHAGNALLVYFV
                     ARRLLAAADDDGAQKTRSERDVSVAAALAALVFGLHPLRVEPVAWIAARADLLCGAFA
                     LLAVWLYLRAVDEPPRPRLLLVAAGALAAALLSKGAALPLVAALLLLDVYPLRRLRRV
                     GGWALLVEKIPIMLVTLGGTALVVWALREGAVINRGAEYGFLARASVAAYSFVIFPVR
                     FVWPFSLSPLYEMPRAISVLEPRFGLALVATAAVTAALIVLRRRWPAGLSVWAFSALM
                     LAPASAAVRLGVDLAPDRYSYLSGLGFAMLAGGAALGAIRLARRHPLARPVRWARIFA
                     TGVVLGGFGLTSWSFAEVWRDSEPLWRWA"
BASE COUNT          255 a          790 c          728 g          382 t
ORIGIN      
        1 ctgatcctgc cgatcagcgg cgcggtgcat gccggcttcc agctcgcgca cgaccgctac
       61 agctaccttt cgggcctcgg cttcgcgctc gtggcgggtg gggcgatcgc ctgggttctg
      121 gcggccgccg gggccaggag agtaggccga ccggtgctcg cggcgggtct cggggtcgcc
      181 actctcgtga tcggcgcgct cgcggtgcag agctggcagc agctgcgcat ctggcgagat
      241 tcagacaccc tctggcgctg gagcctcgag gcagatcccc gctgcgccgt ctgtgccaac
      301 aatctggccg ccctgatcat caaccaaccg tcgccgaagc cggcgcagct caccgaggcg
      361 gagagtctgg cccgacgggc gctcgctcag agacccacgt acgacagtgc gcacaatacg
      421 ctcggcgtcg tgttcgccca gcggggcgag gacggggccg ccgagaccga attccgccag
      481 gcgatacagc tctcgcccga tcgggtcgct tccgtcgcca atctcggcgc gctgtacgcg
      541 cggaccggcc ggcccgagga ggccttgccg ctgcttcgcc gggcatgggc caaggagccc
      601 gactatccgg gattgcggac gagcctcggc ttcaccctcc gcgacgcggg gatcgttcag
      661 gcgcgggccg gccacctgcc cgcggccatc ggcctgctgc aggaggccga gcgggagatc
      721 cccgacgacg cggacgttca tcgcaacctc gggctcgccc tctgggagca cggccagagc
      781 gccgccgcgg tcccctacct ggagcgcgcg gtgacgctcc ggcccgacga cgagaatggc
      841 cggcgcctgc tgacacgcct ccgaaccgat cccgagcatc ctccctcgtt ccgatgagcc
      901 accgctggat actcccggtc tcggtcaccg tcctgaccgt catctgcttc ctgcccgcgc
      961 tgaccggttc gttcctcaac tgggatgaca acgtgaactt cctggagaac accgcttacc
     1021 ggggactcgg gccggcccag gtccgttggg cattcaccag tgtcctcttc ggccactaca
     1081 tccccctgac tcgcctcacg tggagcctga actacgtcct gggcggcttg gatccgagcg
     1141 gctaccatct cgtgaacctg ctcctccacg cgggcaatgc cttgctggtc tacttcgtgg
     1201 ctcgtcggtt gctggccgcc gctgacgacg acggcgcgca gaagacgcga tccgagcgag
     1261 acgtgagcgt ggccgccgcg ctcgccgcgc tcgtcttcgg gcttcaccca ctccgggtcg
     1321 agccggtcgc ctggatcgcg gctcgggcgg acctcctctg cggggccttc gcgctcctgg
     1381 cggtgtggct ctatctacgg gcggttgacg agccaccccg ccctcggctc ctgctcgtcg
     1441 cagcgggagc cctcgcggcc gccttgctct ccaagggcgc ggccctcccc ctggtggccg
     1501 ctctcctcct cctcgatgtc tatcccctgc gccgtctgcg ccgggtcggc ggatgggctc
     1561 tcctcgtgga aaagattccg atcatgctcg tgacattggg cgggacggcg ctcgtcgtgt
     1621 gggcgctgcg ggaaggcgcc gtcatcaacc ggggggcgga gtacggcttc ctcgctcggg
     1681 cttcggtcgc ggcctacagc ttcgtgatct ttccggtccg attcgtctgg cccttctccc
     1741 tgtcccccct ctatgagatg ccccgcgcga tcagcgtgct ggagccgcga ttcggcctgg
     1801 ccctggtggc cacagccgcg gtcacggccg ccctgatcgt cctgcggcgc cgatggcccg
     1861 ccggtctgag cgtctgggcc ttctccgcgc tgatgctcgc tcccgcgagc gcggcggtgc
     1921 ggctcggcgt ggacctcgcc cccgaccggt acagctatct gtcaggattg ggcttcgcga
     1981 tgctggcggg cggtgccgcg ctcggggcga tccggctcgc ccgccgtcac cccttggcgc
     2041 ggccagtcag gtgggccagg atcttcgcga cgggcgtcgt cctgggcggc ttcggtctca
     2101 cctcctggag ctttgccgag gtctggcggg actcggagcc gttgtggcgc tgggc
//
LOCUS       DATMCV010000819         4669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_643157, whole genome shotgun sequence.
ACCESSION   DATMCV010000819 DATMCV010000000
VERSION     DATMCV010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643157"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VNU03_18845"
     CDS             <1..1279
                     /locus_tag="VNU03_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP76586.1"
                     /translation="NLKPLDVRKVDITTVINRIQPKLVAANGLTLYLQPVQDLTVEDR
                     VSRTQYQYSLEDPSGDELNTWTNKLVTRLKTIPALRNVATDQQNDGWEEHIVIDRLTA
                     SRLGITPNLIDNTLYDAFGQRQVSTMFTQLNQYRVVLEVKPEFRRSAGALRDIYVTGT
                     TPGANGPQPVAVPLDTMIRTYPTTAPLVVSRQGQFPAVTLSFNLARGAALGDAVAAVE
                     ATTHQLGLPASIQGSFQGTAQAFRAALANEPLLILAALVAVYIVLGVLYESYIHPLTI
                     LSTLPSAGVGAILALLVCREELSVIALIGIILLIGIVKKNAIMMIDFALEAERGQGQS
                     PGDAIYQACLLRFRPIMMTTMAALLGALPLALGRGVGSELRRPLGIAIVGGLIVSQLL
                     TLYTTPVIYLAFDRLARRFRRAPAEAAEPTESA"
     gene            1276..4407
                     /locus_tag="VNU03_18850"
     CDS             1276..4407
                     /locus_tag="VNU03_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWP76587.1"
                     /translation="MNISAPFIRRPVATTLLTIGLALSGILAWGLLPVSPLPQVDFPT
                     IQVSATLPGAEPDTMATSVAAPLERQFGRIAGLTEMTSTSYLGSTSVALQFDLSRNID
                     GAARDVQAAINAARGYLPPALPNNPSYRKVNPADAPIMIVALTSDTLDRGRMYDAASS
                     ILQQKLSQLPGVGQVIVGGSSLPGVRVEMNPTALNRYGIALETIRSALAATTQYRPKG
                     QLGDETRASEIASNDQLLRAADYAPVIVAFRGGRPVQLSDVARVEDSVEDVRTTGLAN
                     GRPAVLLVIFRQPGANIIDTVDGVRRLLPELGAAIPPAITMSVVMDQTQTIRASLHDV
                     ELTLVISVLLVTLVVFAFLRSVRATLIPAVAVPVSLVGTFGVMYVLGYSLDNLSLMAL
                     TIATGFVVDDAIVVLENVARHRAAGLSPLEAALRGAREIGFTVLAISLSLVAVFTPIL
                     LMGGLVGRLFREFAMTLSAAIAISLLVSLTTTPMMCATLLARESSEHGRLYRASERAF
                     EAMRRGYETSLAWVLRHPRGVLAVTLLTVALNGYLFWVIPKGFFPQQDTGRLQGAIQA
                     AQDISFQAMQAKLTEIVDIVGHDPAVANVVGFTGSQGNGPSTATNTARLFVMLKPLAE
                     RKISADQVITRLRGRVAGVPGAPAFFQAVQDVRAGGRMGNAQYQYTLQGDDVRELDAW
                     GPRVAERLRALPQLTDLGSDQQDRGRQSSLVIDRATASRLGITPQLIDDTLDDAFGQR
                     QVAIMYSPLNQYHVVLEVEPRFWQRPETLGDIYLPSAKGGMVPLSAFSRYEPTQTALA
                     VNHQAQFPAVTLTFNLAPGVALGDAVTAIEAAVRPLGLPASIQASFQGTAQIFQASLA
                     SQPWLIAAALLAVYVVLGVLYESYIHPLTILSTLPSAGVGALLALLACRSDLTVIALI
                     GIVLLIGIVKKNAIMMIDFALAAERGEGKSPAEAIYQACVLRFRPIMMTTMAALLGAL
                     PLALGRGIGSELRRPLGIAIVGGLIVSQALTLYTTPVVYLYLDRVRLWWARGRAGRGR
                     RLASTAAPDALEP"
     gene            4404..>4669
                     /locus_tag="VNU03_18855"
     CDS             4404..>4669
                     /locus_tag="VNU03_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HWP76588.1"
                     /translation="MRRRTVCGRLGGPLALASALLVLVSACTVGPDYVRPSMISPDAY
                     KELDGWKIAHPRDGLARGPWWEIFADPQLNALEARVSISNQNLAV"
BASE COUNT          675 a         1667 c         1597 g          730 t
ORIGIN      
        1 caacctgaag ccgctcgacg tgcgcaaagt cgatatcacg acggtcatca accgcattca
       61 gccgaagttg gtggccgcca atggcttgac gctgtacctg cagccggtgc aggacctcac
      121 cgtcgaagat cgcgtgagcc gcacgcaata tcagtacagc cttgaagatc cgagcggcga
      181 cgagctgaac acctggacca acaagctggt caccaggctc aagacgattc ccgcgctgcg
      241 caacgtcgcg accgatcagc agaacgacgg ctgggaggag catatcgtca tcgatcggtt
      301 aacggcgtcg cggctcggta tcacgccgaa tctgatcgac aacacgctgt atgacgcctt
      361 cgggcagcgg caggtgtcca cgatgttcac ccagctcaac cagtaccggg tcgttctcga
      421 ggtcaagccc gagttccgcc ggagcgcggg agccctccgg gacatctacg tcaccgggac
      481 gacccccggc gcgaatggtc cccagccggt cgcggtgccg ctcgacacca tgatccggac
      541 ctaccccacc acggccccgc tcgtggtgtc gcggcagggg cagttcccgg cggtgaccct
      601 gtccttcaat ctggcccgcg gggcggccct cggcgatgcg gtcgcggcgg tggaggcgac
      661 gacccaccag ctcgggctgc ccgcgagcat ccaggggagc ttccagggga cggcgcaggc
      721 gttccgggcc gcgctggcca acgagccctt gctgatcctg gccgcgctgg tggcggtcta
      781 catcgtgctg ggcgtcctct acgagagcta catccacccg ctcaccatcc tctccacgct
      841 gccctcggcg ggagtggggg cgatcctcgc gctcctggtt tgccgcgagg agctgagcgt
      901 catcgcgctg atcggcatca tcttgttgat cggcatcgtg aagaagaacg cgatcatgat
      961 gatcgatttc gccctggagg cggagcgcgg gcagggccag agccccgggg acgccatcta
     1021 ccaggcctgc ctcctgcggt tccgcccgat catgatgacc acgatggcgg cgctgctggg
     1081 ggcgctcccg ctcgcgctcg gaaggggagt gggctccgag ctccgccggc cgctcggcat
     1141 cgccatcgtg ggcggactca tcgtgagcca gctgctcacg ctctacacca cgccggtgat
     1201 ctatctcgcc ttcgaccggc tggcgcggcg cttccggcgg gcgccggcgg aggctgcgga
     1261 gccgacggag tcggcatgaa catctccgcg cccttcatca ggcggccggt ggccaccacg
     1321 ctgctgacga tcggcctggc cctgagcggg atcctcgcct ggggcctgct gccggtctcg
     1381 ccgctgcccc aggtggattt tccgacgatc caggtcagcg cgacgctgcc gggtgcggag
     1441 ccggacacga tggcgacgtc ggtggccgcg ccgctcgagc gccagttcgg ccgcatcgcg
     1501 gggctcaccg agatgacgtc gaccagctat ctgggctcga cgagcgtcgc gctccagttc
     1561 gatctgtccc gcaacatcga cggcgcggcc cgcgacgtcc aggccgcgat caacgcggcc
     1621 cgcggctacc tgccgcccgc gctgcccaac aacccgagct accgcaaggt gaacccggcc
     1681 gacgcgccga tcatgatcgt ggcgctcacc tcggacaccc tcgatcgcgg ccggatgtac
     1741 gacgcggcct ccagcatcct gcagcagaag ctgtcccagc tgccgggggt gggccaggtg
     1801 atcgtgggcg gcagctcgct gccgggggtc cgggtggaga tgaaccccac cgcgctcaac
     1861 cgctacggca tcgcgctcga gacgatccgg agcgcgctcg ccgcaacaac ccagtatcgg
     1921 cccaagggac agctgggcga cgagacccgg gcctcggaga tcgcgtccaa cgatcagctc
     1981 ctccgggccg ccgactacgc cccggtgatc gtcgccttcc gcggcggtcg gccggtgcag
     2041 ctctcggacg tggcgcgcgt cgaggactcg gtcgaggacg tccgcaccac cggcctggcg
     2101 aacggccggc ccgcggtgct gctggtgatc ttccgccagc cgggggccaa catcatcgac
     2161 accgtcgacg gggtgcgccg cctgctgccg gagctcgggg ccgcgatccc gccggccatc
     2221 accatgtcgg tggtgatgga ccagacccag accatccgcg cgtcgctgca cgacgtcgag
     2281 ctgacgctgg tgatctcggt gctcctggtg accctcgtcg tgtttgcctt cctgcgatcc
     2341 gtccgggcca ccctcattcc cgcggtggcg gtgccggtct cgctggtggg caccttcggg
     2401 gtgatgtacg tgctcggcta cagcctggac aacctgtcgc tcatggcgct gaccatcgcg
     2461 accggattcg tggtggacga cgccatcgtg gtcctggaga acgtggcgcg gcaccgggcg
     2521 gccggtctct cgcccctcga ggccgcgctc cggggggccc gggagatcgg cttcacggtg
     2581 ctcgccatca gcctctcgct ggtggcggtc ttcacgccca tcctcctgat gggcggcctg
     2641 gtgggccgcc tcttccggga gttcgcgatg acgctctccg ccgcgatcgc gatctcgctg
     2701 ctggtgtcgc tgaccactac cccgatgatg tgcgccaccc tgctcgcccg cgagtcgagc
     2761 gagcacggcc gcctctaccg ggcgagcgag cgggcgttcg aggcgatgcg gcgcggctac
     2821 gagacgagcc tcgcgtgggt gctgcgccat ccgcgcgggg tgctcgcggt gacgctcctg
     2881 accgtggccc tgaacggcta tctcttctgg gtgatcccca agggcttctt cccccagcag
     2941 gacaccgggc gcctgcaggg ggcgatccag gccgcgcagg acatctcctt ccaggcgatg
     3001 caggccaagc tgaccgagat cgtggacatc gtcgggcacg acccggcggt ggcgaacgtg
     3061 gtgggattca cgggcagcca gggcaacggg cccagcaccg ccaccaacac cgcgcgcctg
     3121 ttcgtcatgc tgaagccgct cgcggagcgg aagatcagcg ccgaccaggt gatcacgcgg
     3181 ctgcgaggcc gggtggcggg agtgccgggg gcccccgcct tcttccaggc ggtgcaggac
     3241 gtccgcgcgg gcgggcgcat ggggaacgcg cagtaccagt acacgctcca gggggacgac
     3301 gtgcgggagc tggacgcgtg gggcccgcgg gtcgcggagc gccttcgcgc gctgccgcaa
     3361 ctcaccgatc tgggcagcga tcagcaagac cgggggcggc agtcgtcgct ggtgatcgac
     3421 cgggccaccg catcgcgcct cggcatcacc ccccagctca tcgacgacac gctcgacgac
     3481 gccttcggcc agcgccaggt tgcgatcatg tatagcccgc tcaaccagta tcacgtggtg
     3541 ctggaggtcg agccgcgctt ctggcagcgt cccgagaccc tcggggacat ctacctcccg
     3601 tccgccaagg gcggcatggt gccgctcagc gcattcagcc gatacgagcc cacccagacc
     3661 gcgctggcgg tgaaccacca ggcgcagttc ccggcggtga cgctgacctt caacctggct
     3721 cccggcgtcg cgctgggaga cgcggtgacc gcgatcgagg ccgcggtgcg tccgctcggg
     3781 ctgcccgcct cgatccaggc tagcttccag ggcaccgccc agatcttcca ggcctcgctg
     3841 gccagccagc cctggctgat cgccgcggcg ctcctcgcgg tgtacgtcgt cctcggggtg
     3901 ctctacgaga gctacatcca cccgctcacc atcctgtcca cgctgccgtc ggccggggtg
     3961 ggcgcgctgc tggcgctgct ggcgtgccgc agcgatctga ccgtcatcgc cctgatcggc
     4021 atcgtcctgc tcatcggcat cgtgaagaag aacgcgatca tgatgatcga cttcgcgctc
     4081 gcggccgagc ggggagaggg caagagcccg gcggaggcga tctatcaggc ctgcgtgctg
     4141 cgcttccggc cgatcatgat gacgacgatg gccgcgctgc tgggcgcgct cccgctggcc
     4201 cttggccgtg gcatcgggtc ggagctgcgc cggccactcg ggatcgccat cgtgggcggg
     4261 ctcatcgtga gccaggcgct tacgctctac acgacgccgg tcgtgtacct ctacctcgac
     4321 cgcgtacggc tgtggtgggc gcgcgggcgc gccggacgtg ggcggcggct cgcctccacc
     4381 gcggccccgg atgcgctgga gccatgaggc gccggacggt ttgcggacgc ctcggcggcc
     4441 cgctcgcgct cgcctccgcg ctgctcgtgc tcgtgagcgc ctgcaccgtg gggcccgact
     4501 acgtcaggcc gtcgatgatc agccccgacg cctacaagga gctcgacggc tggaagatcg
     4561 cccatcctcg ggacggcctg gcccgcggcc cctggtggga gatcttcgcc gatccgcagc
     4621 tgaacgccct cgaggcgcgc gtcagcatct cgaaccagaa cctcgcggt
//
LOCUS       DATMCV010000820         2420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_447982, whole genome shotgun sequence.
ACCESSION   DATMCV010000820 DATMCV010000000
VERSION     DATMCV010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447982"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..480)
                     /locus_tag="VNU03_18860"
     CDS             complement(46..480)
                     /locus_tag="VNU03_18860"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWP76589.1"
                     /translation="MRSSAYRRLLVLLDGTERGERALTWARHLARGPGSAVHLLMIEP
                     AARMLGVGGRTVAFVDQLEDAARAAARAYLASVAGRLREDGMTVWTHVRFGAAAPVTR
                     AVIQELEADVLVVTDGVIRRPDDLGVVPIPVLTSGPRCLRSA"
     gene            609..1514
                     /locus_tag="VNU03_18865"
     CDS             609..1514
                     /locus_tag="VNU03_18865"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWP76590.1"
                     /translation="MADAPLPTRDRVAAYFKELNNWGRWGHDDQLGTVNLITPAKQAQ
                     AQRLIKKGRTVSLARDVVPRPVYMYHMTFPSKRERVDVVLDRFDMVYHGYWITHVDAL
                     CHVGWDGELYNGRPFAESLTVEGALWCPIDPIFTRGITTRGVFLDVAAGRKEGYVTVG
                     RPVTPRELDETERRAGVKVEPGDVVVLRSGDEKFRLENPDWKPRISEHPGLNVSCLEW
                     FREKDIAAISWDMMDERPSGYPGFGMSTHLAIPFLGLALIDNSDPERLAKACAEEGRN
                     EFLFTATPLRLVGATGAPAHPIAIF"
     gene            1514..>2420
                     /locus_tag="VNU03_18870"
     CDS             1514..>2420
                     /locus_tag="VNU03_18870"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76591.1"
                     /translation="MSRHVPGRSIRPLALVALLFLATAASALAAEGEMRWGLHVTLAP
                     KWLDPAETEAFSTPFMVLYAIHDALVKPMPGGLNTPSLAQSWQESKDHLSFTFNLRKN
                     ARFHNGEPVTAEDVKFSFERYKGASATLLKEKVKEVQVLGPAQVRFVLKEPWADFMTF
                     YGTTASGAAWIVPKKYVQQVGEDGFKAAPVGAGPYKVVSFKPGVEIVMEAFDGYWRKA
                     PSVKRLVMRSMPEETTRAAALKSGEVDIAYLLTGPTAEAIRKTAGFTLKAPLLSGAFW
                     LELPEQWDPKSPWHDLRVRQAASLAI"
BASE COUNT          382 a          835 c          848 g          355 t
ORIGIN      
        1 gccggtcggg tgagcagggt cttgatctgc atgggctcct ctcttctatg cggaccgcaa
       61 gcatcgcggg ccgctcgtca gcacgggaat cgggacgacg ccaagatcat ccgggcgtcg
      121 aatcacgccg tcggtcacga cgaggacgtc ggcctccagc tcctggatga cggcgcgggt
      181 caccggggcg gccgcgccga agcgcacgtg ggtccacacc gtcatgccgt cttcccgcag
      241 gcggccggcg accgacgcga ggtaggcgcg cgcggcggcc cgggcagcgt cctcgagctg
      301 atcgacgaag gccaccgtcc gcccgccgac gcccagcatg cgcgcggccg gctcgatcat
      361 caggaggtgg accgcgctgc cgggtccccg ggccaggtgg cgcgcccagg tcagggcgcg
      421 ctcgccgcgc tcggttccgt ccagcagaac gagaagacgt cggtaagcgc tcgatcgcat
      481 gcgtccaggg taccgaccgg ctcgacgccg gctcgctaag ctttcgtgac gtgccggccc
      541 gggccggatc gtgctacctt cctgccaacg atcccgcgag atcgcacgaa tacgaaagga
      601 tccgaccgat ggccgatgcc ccgctgccga cccgtgatcg cgtcgccgcc tacttcaagg
      661 agctgaacaa ctggggccgc tgggggcacg acgaccagct cggcaccgtg aacctgatca
      721 cgccggccaa gcaggcgcag gcccagcggc tgatcaagaa gggccgcacc gtgtcgctgg
      781 cgcgcgacgt ggtgccgcgg ccggtgtaca tgtaccacat gacgtttccg tccaagcggg
      841 agcgggtgga cgtggtgctc gaccgcttcg acatggtcta ccacggctac tggatcaccc
      901 acgtggacgc gctctgccac gtgggctggg acggcgagct ctacaacggc cgcccgttcg
      961 cggagagcct caccgtggag ggcgcgctct ggtgcccgat cgacccgatc ttcacccgcg
     1021 gcatcaccac gcgcggggtg ttcctcgacg tggcggccgg gcgcaaggag ggctacgtca
     1081 cggtgggccg tccggtgacc ccgcgcgagc tcgacgagac ggagcggcgc gcgggcgtga
     1141 aggtggagcc gggcgacgtg gtggtgctgc gcagcggcga cgagaagttc cgcctcgaga
     1201 acccggactg gaagccgcgg atctcggagc acccgggcct gaacgtgtcc tgcctcgaat
     1261 ggttccgcga gaaggacatc gcggcgatct cctgggacat gatggacgag cggccgagcg
     1321 gttaccccgg cttcggcatg tccacccacc tggcgatccc gttcctgggc ctggccctca
     1381 tcgacaactc ggaccccgag cgtctggcca aggcctgcgc cgaggaaggg cgcaacgagt
     1441 tcctgttcac ggccacgccg ctgcgcctgg tcggcgccac cggcgcgccc gcgcacccga
     1501 tcgcgatctt ctgatgagcc gacacgtccc gggccgatcc atccggccgc tcgctctcgt
     1561 cgcgctcctc ttcctcgcga ccgccgcctc cgctttggca gctgaaggcg agatgcgctg
     1621 gggcctgcac gtcacgctcg cccccaagtg gctcgatccg gccgagaccg aggcgttcag
     1681 cactccgttc atggtgctct acgcgataca cgacgcgctg gtgaagccga tgccgggcgg
     1741 gctcaacacc ccgagcctgg cccagtcgtg gcaggagtcg aaggaccatc tctcgttcac
     1801 gttcaacctc cgcaagaacg cgcgcttcca caacggagag cccgtcaccg cggaggacgt
     1861 gaagttctcc ttcgagcgct acaagggggc cagcgccacc cttttgaagg agaaggtgaa
     1921 ggaggtgcag gtgctggggc cggcccaggt gcgcttcgtg ctgaaggagc cgtgggccga
     1981 cttcatgacc ttctacggga cgaccgcctc gggggcggcc tggatcgtgc ccaagaagta
     2041 cgtgcagcag gtgggcgagg acggcttcaa ggccgcgccg gtcggggcgg ggccctacaa
     2101 ggtggtctcg ttcaagcccg gcgtcgagat cgtcatggag gccttcgacg gctactggcg
     2161 gaaggcccca tcggtgaagc gcctggtcat gcgctcgatg ccggaggaga ccacccgcgc
     2221 ggccgcgctc aagagcggcg aggtggacat cgcgtatctg ctcaccgggc cgaccgcgga
     2281 ggccattcgc aagacggccg gcttcaccct gaaggcgccg ctgctctcgg gcgcattctg
     2341 gctcgagctg cccgagcagt gggacccgaa gtcgccctgg cacgatctgc gggtgcgcca
     2401 ggcggcgagc ctcgcgatcg
//
LOCUS       DATMCV010000821         3576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_111106, whole genome shotgun sequence.
ACCESSION   DATMCV010000821 DATMCV010000000
VERSION     DATMCV010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111106"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VNU03_18875"
     CDS             <1..257
                     /locus_tag="VNU03_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HWP76592.1"
                     /translation="IFGVLGYALRKLRVDPSPMVVALVLGPMMEKTLRQALFLTRGSV
                     VDLVARPLSATILILAAAALLGPPLVRLLRRGRVAQPLTR"
     gene            279..1463
                     /locus_tag="VNU03_18880"
     CDS             279..1463
                     /locus_tag="VNU03_18880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP76593.1"
                     /translation="MRLSLAFEMQRPVVDDHAVVEETIEQCVLADRMGFDAVWFVEHH
                     FLTSFSMSPCPEVIFGALSRLTKRIRLGFGVVILPYHHPVRVAERVAMVDHLSRGRVE
                     FGTGRSAAYEQIGMGIDPRQTRAMWEESLAMIPKIWEDGPFSWEGQFWSVPPRDVRPK
                     PYQKPHPRMWVAALQPATYQLAAEKGIGVMALSVAAPSYLEPHIKAYKERVRHASPVG
                     KVVNDQWLSSTMGLCDADDKAARELSAKSLRTFFGPDRPYLKDQTHLYEQLVDSWGGV
                     PDHLKANFARYLKTEGPGGGPPAGAPEVDLSGGSGQIASALWNQIDADTLVDRGVLVA
                     GNPETCLRSIAIHEAVGVDELQFLMATETVPHDKVMSSIEMFGRHVIPELKKRRKRRG
                     RK"
     gene            1463..2536
                     /locus_tag="VNU03_18885"
     CDS             1463..2536
                     /locus_tag="VNU03_18885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP76594.1"
                     /translation="MDVGLQMIFATYGWSNVSDEQAWDEELRLARLAADLGFDVLWSV
                     EHHGNDYSFCPDNLQLMSYLAAECPEVGLGTAAVILPWHDPLRVAEQASVLDHLSRGR
                     LRLGIGRGLARREFEAFRGTMDESRGRFDEAAEMILRGLRTGFMEGDGPFYRQPRTEI
                     RPRPKRSFDDRVYAVASSDDSVVSAARLNARMVMFADRPWPIRMPAIQKHRELVREIH
                     GKEAGPPLLADFCVCTPTMDGAEETARRYMGKFVESNFYHYELLGPHFATVKGYDAYA
                     QKVAMAREIGMDGIVSAFMQAAVWGTPDRILRTLEERRALVGVFELATSFRFGGTPLD
                     LAESGLRLYAKEVLPIVQSWAGA"
     gene            2571..3398
                     /locus_tag="VNU03_18890"
     CDS             2571..3398
                     /locus_tag="VNU03_18890"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWP76595.1"
                     /translation="MLWSSSFITARVGLRVVSPLLFVGVRLVAAAALLGLAALVGRHS
                     WAALRGRWHHLAVAGALVNGVTLSAFHVGMVTENVAVMALLQSLGPMLIALSAVPLLG
                     ERLRSSQWLGLILGTAGVMMVVAPRALGSPAGWRAIALGLVGVAGLAGGTIYFRRFCA
                     GVPLLTATTVQVAAGALLTVALALGFEHLHAVWTVPAVASVVWNVLAVSIGAMALYYY
                     LLARGTAGRVAANFYLVPGTVAVAAWALLGERLTPLAVLGFALASAGVFLVARVPGP"
     gene            complement(3395..>3576)
                     /locus_tag="VNU03_18895"
     CDS             complement(3395..>3576)
                     /locus_tag="VNU03_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020022762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iditol 2-dehydrogenase"
                     /protein_id="HWP76596.1"
                     /translation="LVGSWTFSKQGQAECAEFVADRQVDVEKLFTHRWKLDQAEEAYR
                     LFDTQTTGKAVILPS"
BASE COUNT          458 a         1281 c         1261 g          576 t
ORIGIN      
        1 tgatcttcgg cgtgctcggc tacgcgctgc ggaagctccg cgtggacccc tccccgatgg
       61 tggtcgcgct ggtgctgggg ccgatgatgg agaagacact gcgccaggcg ctcttcctca
      121 cgcgcggcag cgtggtcgat ctcgtggcgc gcccgctgtc ggcgacgatc ctgatcctcg
      181 ctgccgccgc gctcctcggg ccgccgctcg tccggctgct gcgccgcggg cgagtcgccc
      241 agccgctcac gcggtaaccg ggtacgggag gccggcgcat gagactgagc ctggcgttcg
      301 agatgcagcg gcccgtggtc gacgaccacg cggtcgtcga ggaaacgatc gagcagtgcg
      361 tgctcgcgga ccggatgggc ttcgacgcgg tctggttcgt ggagcatcac ttcctcacca
      421 gcttctcgat gtctccgtgc ccggaggtga tcttcggggc gctcagccgg ctgaccaagc
      481 ggatccgcct cggattcggc gtggtgatcc tcccctacca ccacccggtg cgcgtggccg
      541 agcgggtggc gatggtcgat cacctctcgc gcggccgggt cgagttcggc accgggcggt
      601 cggcggcgta cgagcagatc ggcatgggca tcgacccgcg ccagacccgc gcgatgtggg
      661 aagagtcgct cgccatgatc ccgaagatct gggaggacgg ccccttctcc tgggagggac
      721 agttctggag cgtcccgccg cgcgatgtgc gcccgaagcc ctaccagaag ccgcacccgc
      781 gcatgtgggt ggccgcgctc cagcccgcga cgtaccagct cgcggccgag aagggcatcg
      841 gggtgatggc gctcagcgtg gccgcgcctt cctatctcga gccccacatc aaggcgtaca
      901 aggagcgggt gcgccacgcg agcccggtgg gcaaggtcgt caacgaccag tggctgtcct
      961 cgacgatggg gctctgcgac gccgacgaca aggcggcgcg cgagctgtcg gcgaagtcgc
     1021 tgcgcacctt cttcgggccg gatcgcccgt acctcaagga tcagacgcac ctctacgagc
     1081 agctcgtcga ctcctggggc ggcgtgcccg accatctcaa ggccaacttc gcccgctacc
     1141 tcaagacgga aggccccggc ggcgggccgc ccgccggtgc gccagaagtc gatctgtccg
     1201 ggggctccgg gcagatcgcg agcgcgctgt ggaaccagat cgacgccgac accctggtcg
     1261 accgcggcgt cctggtggcg ggcaatcccg agacctgcct ccggtccatc gccattcacg
     1321 aggcggtcgg ggtggacgag ctgcagttcc tcatggcgac ggagacggtg ccccacgaca
     1381 aggtcatgtc gtccatcgag atgttcggcc ggcatgtcat cccggagctg aagaagcggc
     1441 ggaagcgccg cgggagaaag tgatggacgt cggcctgcag atgatcttcg ccacctacgg
     1501 gtggagcaac gtgtccgacg agcaggcgtg ggacgaggag ctgcggctcg cgcgactggc
     1561 cgccgatctc ggcttcgacg tcctgtggtc cgtcgagcat cacgggaacg actactcgtt
     1621 ctgcccggac aacctccagc tcatgtccta cctggccgcc gagtgccccg aggtcggcct
     1681 cgggaccgcc gcggtgatcc tgccctggca cgaccccctc cgcgtcgccg agcaggcgtc
     1741 cgtcctcgac cacctgagcc ggggccggct ccgcctcggc atcgggcgcg gcctcgcccg
     1801 gcgggagttc gaggccttcc ggggcacgat ggacgaatcg agggggcggt tcgacgaggc
     1861 cgcggagatg atcctccgtg gcctccgcac cggcttcatg gagggcgacg ggcccttcta
     1921 ccggcagccg aggaccgaga tccggccgcg tccgaagcgg agcttcgacg accgcgtgta
     1981 cgcggtggcc tcctcggacg actcggtcgt ctccgcggcc cggctgaatg cccgcatggt
     2041 catgttcgcc gaccggccct ggcccatccg catgccggcc atccagaagc accgcgagct
     2101 ggtgcgggag atccacggca aggaggcggg gccacccctg ctggccgact tctgcgtctg
     2161 cacgcccacc atggacgggg cggaggagac ggcgcgccgc tacatgggga agttcgtgga
     2221 gagcaacttc taccactacg agctgctggg cccccacttc gccaccgtca agggctacga
     2281 cgcctacgcg cagaaggtcg cgatggcccg ggagatcggc atggacggga tcgtctccgc
     2341 cttcatgcag gccgcggtct ggggcacgcc cgaccggatc ctgcgcacgc tcgaggagcg
     2401 acgggccctc gtgggcgtct tcgagctggc cacgtcgttc cgcttcgggg gcacgcccct
     2461 cgacctggcc gagtccgggc ttcggctcta cgccaaggag gtcctgccga tcgtccagtc
     2521 ctgggccggg gcgtagtcgt atcgcgcgca tcgccccgta cgtcttcgtc ctgctctgga
     2581 gctcctcctt catcacggcg cgcgtcggcc tccgcgtggt ctcgccgctg ctcttcgtcg
     2641 gcgtgcgact ggtggccgcc gccgccctcc tgggcctcgc cgccctggtg ggccggcact
     2701 cctgggccgc cctccgcggg cgctggcatc acctcgcggt ggccggcgcc ctcgtcaacg
     2761 gcgtcaccct ctccgcgttt cacgtgggga tggtgaccga gaacgtcgcg gtgatggcgc
     2821 tgctgcagtc gctcggcccg atgctcatcg ccctctcggc ggtgccgctg ctcggggagc
     2881 gcctccgctc ctcgcagtgg ctcggcctca tcctgggcac cgccggcgtg atgatggtcg
     2941 tggcgccgcg cgcgctcggg agccccgcgg gctggcgggc catcgcgctc gggctcgtcg
     3001 gcgtcgccgg cctcgccggc ggcacgatct acttccgccg cttctgcgcg ggcgtgcccc
     3061 tgctcaccgc caccaccgtg caggtcgccg cgggcgcgct cctgaccgtg gcgctggcgc
     3121 tgggattcga gcacctgcac gcggtgtgga cggtccccgc ggtggcctcc gtggtctgga
     3181 acgtgctggc ggtctcgatc ggagccatgg cgctctacta ctacctgctc gcgcgcggga
     3241 cggcgggccg cgtggcggcc aacttctacc tcgtccccgg gacggtggcg gtggccgcgt
     3301 gggcgctcct gggggagcgc ctgacgccgc tggccgtcct cggcttcgcc ctcgcctcgg
     3361 cgggcgtgtt cctggtggcc cgcgttcccg ggccctagga cggcaggatg accgccttgc
     3421 ccgtcgtctg cgtgtcgaag agccggtagg cttcctccgc ctgatcgagc ttccaacggt
     3481 gggtgaagag cttctccacg tccacctgcc ggtcggccac gaactcggcg cactcggcct
     3541 ggccctgctt cgagaacgtc caggagccca cgagcg
//
LOCUS       DATMCV010000822         2718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_839981, whole genome shotgun sequence.
ACCESSION   DATMCV010000822 DATMCV010000000
VERSION     DATMCV010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839981"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1497)
                     /locus_tag="VNU03_18900"
     CDS             complement(148..1497)
                     /locus_tag="VNU03_18900"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWP76597.1"
                     /translation="MDYLDRLSAFVGAVSLDRLPEEAVQAARLVLLDTIGAMVAGSAQ
                     PENRRLAAAMHERRSRAGATLVGHGGRVDPLLATFANATAGVTLEVDEGSRLGGGHPA
                     IHVVPAALAVAEDTGADGRRLLESVIAGYEVCSRLGGATTPKPNVHSHGTWGTIGTAV
                     AVARLAGLDAASVRSVINLSASMSPANTWTTALEGVTIRNAYPGRSGLEGILAVDLHR
                     AGFTGLPDAPSDVYGTILADRFEPERALDGLGGPLRIQQNYFKLYACCRYNHFALDAL
                     LAMTRAGEVRAEEVERVVVTTIPFGPRMAGTAPASMLGAKFSIPYAVAAALVLGRADV
                     SAFVEPALGDPRIRALAARVEVRVDPEMSPRRADAPTARVEVALKDGRALTGVTSVVR
                     GDFEDPVPAAEVVEKFVALTSDVLGIGRARIVVQIVGDVGTLKNLHDLTELLAPATS"
     gene            complement(1513..>2718)
                     /locus_tag="VNU03_18905"
     CDS             complement(1513..>2718)
                     /locus_tag="VNU03_18905"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76598.1"
                     /translation="DWHTGPVAAAHAGLTSLLSFVEYDDQAKETLPEALKRLRDEAGS
                     QSVVDFGFHFILNNQPYILDGLAEAFEQGVSSYKIFMTYKKRPNRMCSDEFIAQVMEK
                     IAALGGVCQLHCENGDILYHLENKSIAAGKVKPTDFPATCPPWTEEEAINRAILIGEM
                     TKCPVYVVHLSTKVGLERIKAAQARGQRVWTETCPQYLLLTEKEMEAWGPLAKIGPPL
                     RPVGGGDGDALWGGSREGYIATVASDHSPRRKELKEPGWKNIFVDGKGNPVPFGSPSI
                     ETLVPLMWSEGVVKRGLPVTWLARVMAENPARIFGLYPRKGAIRVGADADLTIIDPDA
                     DGTIQVADHHGVAGWTLYEGWKSKGRPWMTVLRGQVLLNQGRLEQKPGYGQFLARTGP
                     VAPLGGTAG"
BASE COUNT          377 a          991 c          946 g          404 t
ORIGIN      
        1 ccggcctcgc gatcttctcg ctgacgctgt cgttcaacct ggccggcgac ggcgtccgcg
       61 acctgctcga ccccaaggac tactagcttc cccctctccc ctccggggag agggcagggt
      121 gagggggccg ctccagggat cgctgattta cgacgtcgcc ggcgccagaa gctcggtcaa
      181 gtcgtgcagg ttcttgagtg tgccgacatc gcccacgatc tgaaccacga tgcgagcccg
      241 accgatgcca agaacgtccg atgtcagcgc aacgaacttc tccacgacct ctgccgccgg
      301 caccggatcc tcgaagtcgc cgcgcaccac actggtgact ccggtgagcg cgcgcccgtc
      361 cttgagcgcg acctcgaccc gggcggtcgg ggcgtcggcg cgccggggcg acatctccgg
      421 atccacgcgc acctcgacgc gggcggcgag ggcccggatg cgcggatccc cgagcgcggg
      481 ctccacgaag gctgagacgt cggcgcggcc gagcaccagt gcagccgcca ccgcgtacgg
      541 gatggagaac ttggcgccca gcatcgaggc cggcgcggtg ccggccatcc gcgggccgaa
      601 cgggatcgtg gtgacgacca cgcgctcgac ctcctcggcc cgcacctccc cggcgcgcgt
      661 catggcgagc agcgcgtcca gtgcgaagtg gttgtagcgg cagcacgcgt agagcttgaa
      721 gtagttctgc tggatgcgca gcgggccgcc caggccgtcc agcgcgcgct cgggctcgaa
      781 gcggtcggcg aggatcgtgc cgtagacgtc gctcggcgcg tcgggcagcc cggtgaagcc
      841 ggcgcggtgg agatcgaccg cgaggatgcc ctcgaggccc gagcgccctg gatacgcgtt
      901 gcggatcgtc acgccctcga gcgcggtggt ccaggtgttg gccgggctca tgctcgcgga
      961 gaggttgatc accgagcgga cggaggccgc gtcgagcccg gccagccggg ccaccgccac
     1021 cgcggtgccg atggtgcccc acgtcccgtg ggagtggacg ttcggcttcg gcgtggtcgc
     1081 accgccgagc cgggagcaca cctcgtagcc ggcgatgacg ctctcgagca gccggcgccc
     1141 gtcggccccg gtgtcctccg ccaccgcgag ggcggccggt accacgtgga tcgcgggatg
     1201 accgccgccg agccggctgc cctcgtcgac ctcgagcgtg acgcccgcgg tggcgttggc
     1261 gaacgtggcc agcaacgggt ccacccggcc gccgtggccc acgagagtcg cgccggcgcg
     1321 cgaccggcgc tcgtgcatgg cggcggcgag ccgtcgattc tccggctggg cgctgccggc
     1381 caccatcgcg ccgatcgtgt cgaggagcac caggcgcgcc gcctggaccg cctcctccgg
     1441 caggcgatcc aggctcaccg ccccgacgaa cgcggagagc cggtccagat agtccatggg
     1501 gcggcggcgc ccctacccgg ccgtcccgcc gagcggcgcc accggtccgg tccgagcgag
     1561 gaactgcccg tagcccggct tctgctcgag gcggccctgg ttcagcagca cctggccgcg
     1621 cagcaccgtc atccacgggc ggcccttcga cttccagccc tcgtagagcg tccagcccgc
     1681 gacgccgtga tgatcggcca cctggatggt cccgtccgcg tcgggatcga tgatggtgag
     1741 atcggcgtcg gcgcccaccc ggatggcgcc tttccgcgga tagagcccga agatgcgcgc
     1801 cgggttctcc gccatcacgc gggccagcca cgtgaccggc aggccgcgct tcaccacgcc
     1861 ctcgctccac atgagcggca ccagcgtctc gatcgacggg ctgccgaagg ggaccgggtt
     1921 gcccttgccg tccacgaaga tgttcttcca gcccggctcc ttcagctcct tgcgccgcgg
     1981 cgagtggtcg ctcgccacgg tggcgatgta gccctcgcgg ctgccgcccc agagcgcgtc
     2041 gccgtcgccg ccccccaccg gccggagcgg cggcccgatc ttggcgagcg ggccccacgc
     2101 ctccatctcc ttctcggtca gcagcaggta ctgcgggcag gtctcggtcc agacccgctg
     2161 cccgcgcgcc tgcgcggcct tgatccgctc cagcccgacc ttcgtcgaga ggtgcaccac
     2221 gtagaccggg cacttggtca tctcgccgat gaggatcgcg cggttgatcg cctcctcctc
     2281 ggtccacggc gggcacgtgg cgggaaagtc ggtgggcttc accttgcccg ccgcgatcga
     2341 cttgttctcg agatggtaga ggatgtcgcc gttctcgcag tggagctggc agacgccgcc
     2401 cagcgccgcg atcttctcca tgacctgagc gatgaactcg tccgagcaca tgcggttcgg
     2461 ccgcttcttg taggtcatga agatcttgta ggagctgacc ccctgctcga aggcctcggc
     2521 cagcccgtcc aggatgtagg gctggttgtt caagatgaag tggaagccga agtcgacgac
     2581 ggactgcgag cccgcctcgt cgcgtagccg cttgagcgcc tcgggcaggg tctccttcgc
     2641 ctggtcgtcg tactccacga aggacagcag tgaggtcagg ccggcgtggg ccgccgccac
     2701 cgggccggtg tgccagtc
//
LOCUS       DATMCV010000823         4078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_643308, whole genome shotgun sequence.
ACCESSION   DATMCV010000823 DATMCV010000000
VERSION     DATMCV010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643308"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1108
                     /gene="htpX"
                     /locus_tag="VNU03_18910"
     CDS             239..1108
                     /gene="htpX"
                     /locus_tag="VNU03_18910"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HWP76599.1"
                     /translation="MGNLVKTGVLLAALTVLLVLIGGALGGQHGMLLAFVMAMVMNLG
                     SYWFSDKIVLSMYGAKPVDEAQAPGLYRIVRTLATRAGIPMPRVYILPQETPNAFATG
                     RNPQHAAVAVTEGILRVMSEEELEGVLAHELSHVQNRDTLIMAIAATLAGAITYMAHM
                     AQWAMIFGGGRRDSDEEGSGGGGIFGGILMMVLAPLAATLIQLAVSRSREFQADASGA
                     RMAGQPWGLAKALEKLEMASKAEPMQATPATAHLFIVNPLTGGGFATLFSTHPPIAER
                     VARLRAMASSQAR"
     gene            1184..1897
                     /gene="ccmA"
                     /locus_tag="VNU03_18915"
     CDS             1184..1897
                     /gene="ccmA"
                     /locus_tag="VNU03_18915"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HWP76600.1"
                     /translation="MIAAQELRKTYGPTLVLDGVSFALAPGQCLALLGPNGAGKTTLL
                     RLLATLLRPTSGALRVGGIDALREPDRVRPLLGVVAHGSYVYEDLTARENLRFWETMR
                     GGDASPARLSAALAQVELDTVAEERVRTFSSGMRRRLGLARVLLAQARLLLLDEPFTG
                     LDRRGRKWVNEFLQAFKAGGGTAVLATHSFGGGLGVADRIGILGQGRLLLDRPAADLS
                     PEELHRVYESLTEGAEAGA"
     gene            1894..2571
                     /locus_tag="VNU03_18920"
     CDS             1894..2571
                     /locus_tag="VNU03_18920"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HWP76601.1"
                     /translation="MTYARRAWIIFYKDLLVERRSKEQTNALLFFCLLLLFLFQFALG
                     PDRDRLAAALPGLLWLGVILAGLLGLGRSFLAERENDCWEGLRLTPGDKSAIYLGKLA
                     GNCVLMTAVEVVLVVLFGVFYNVDLWTPAAPLALVLALGTIGLAAVGTLFAAMTTQVR
                     ARELLFPVLLLPVQVPLLLATVSATQALLLGQPLADVAHWLKLLAGADAVYVTIGLLT
                     FEFVLEG"
     gene            2585..3226
                     /gene="ccsA"
                     /locus_tag="VNU03_18925"
     CDS             2585..3226
                     /gene="ccsA"
                     /locus_tag="VNU03_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWP76602.1"
                     /translation="MTRALGWLAALGLLAGLAAGFGYAPREAVQGNVQRIMYLHVPSV
                     LTAYLAFGLVLLGSIAYLATRRPGWDRLAGSAAEIGVLFTGLTIVSGSIWGKPTWGTW
                     WTWDARLTSTAVLFCVYVGYLLLRSLIDDPEARGRYAAVVGILGAVNIPIVHFSVKWW
                     RALHQPSTILGPEPSPIDPAIGLALFVNWVAFTLLFAYFLSRRMEIARLEEQT"
     gene            3226..3366
                     /locus_tag="VNU03_18930"
     CDS             3226..3366
                     /locus_tag="VNU03_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76603.1"
                     /translation="MPANWGYVIAAYGIAAVALFGYWRRLARRARALAARPRTAAKTR
                     AA"
     gene            3363..3809
                     /locus_tag="VNU03_18935"
     CDS             3363..3809
                     /locus_tag="VNU03_18935"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HWP76604.1"
                     /translation="MNRKAKFIGGGLVIVAALAYLIYAGVSQSVVYFVTPSELGAAPV
                     AGKSYRLGGMVLPGTLKWDARSLDLRFTLSDGQASVPVRHRGTPPDLFAESRGAVVEG
                     TWSHDGYFQATTILAKHSEEYKAPHDQSQAGYKDLLKTLKGSEGKP"
     gene            3806..>4078
                     /locus_tag="VNU03_18940"
     CDS             3806..>4078
                     /locus_tag="VNU03_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmF"
                     /protein_id="HWP76605.1"
                     /translation="MTPELGYAATVAALILAVYGSGAAALSAVRGRADLRLSAERAAV
                     GVWLLVLTCMALLIYAFLTFDFSVRYVAGNTNLGTPFYYRITAVWGA"
BASE COUNT          529 a         1497 c         1393 g          659 t
ORIGIN      
        1 tgcggaaact ccgcgacgcc ctcgatacgc agaccctcgt cggggatctc ggagacctgg
       61 atgagcacgt gaaggacccc taactttctg tcaattaagg cgttttgcta aagccgcgtc
      121 agtataagcg cgggggtacc ccttgtcaac ctaattgccc ccatgacggc gcacacatgg
      181 ccggttgctt gacggtcctg agggcgactg ctacccttcg gtcgggagga ccaaccccat
      241 gggcaacctc gtgaaaaccg gtgtcttgct ggcagccttg acggtgctgc tcgtgctcat
      301 cggcggggcg ctcggcgggc agcacggcat gctcctggcc ttcgtcatgg cgatggtcat
      361 gaacctgggc agctactggt tctcggacaa gatcgtcctg tccatgtacg gggccaagcc
      421 ggtcgacgag gctcaggccc ccggactcta ccgcatcgtc cgcaccctgg ccacccgggc
      481 cggcatcccg atgccgcggg tctacatcct cccgcaggag accccgaatg ccttcgccac
      541 cggccgcaat ccgcagcacg cggcggtcgc ggtcaccgag ggcatcctgc gggtgatgag
      601 cgaggaggag ctggagggcg tgctggccca cgagctctcc cacgtgcaga accgcgacac
      661 cttgatcatg gccatcgcgg ccaccctggc cggcgccatc acctacatgg cgcacatggc
      721 gcagtgggcg atgatcttcg gcggcggccg gcgcgacagc gacgaggaag ggagcggcgg
      781 cggcgggatc ttcgggggca tcctgatgat ggtgctcgcc cccctggccg cgaccctcat
      841 ccagctcgcg gtctcccgct cgcgcgagtt ccaggccgac gccagcggcg cccgcatggc
      901 cggccagccg tgggggctcg ccaaggcgct cgagaagctc gagatggcct ccaaggcgga
      961 gcccatgcag gccacgcccg cgaccgcgca tctcttcatc gtcaatccgc tgaccggcgg
     1021 cgggttcgcc acgctcttct ccacgcatcc gcccatcgcc gagcgggtgg cgcggctgcg
     1081 cgccatggcc tcgtcccagg ccaggtagct cgatcgccga gccggccgca ctcacgatcg
     1141 caactccggg gagacgggcg accgtctccc ctttttcacg gacatgatcg ccgcgcagga
     1201 gctccgcaag acctacggcc ccacgctcgt gctcgacggt gtgtccttcg cgctcgcccc
     1261 gggccagtgc ctggccctcc tcggccccaa cggcgccggc aagacgacgc tgctccgcct
     1321 cctcgccacg ctcctgcgcc cgacgtcggg cgcgctccgg gtgggtggga tcgacgcgct
     1381 tcgggagccc gaccgggtgc gccccctgct gggcgtcgtc gcgcacggct cgtacgtcta
     1441 cgaggatctg accgcccgcg agaacctgcg cttctgggag acgatgcgcg gcggtgacgc
     1501 ctcccccgcc cgtctgagcg ccgctctggc ccaggtggag ctcgacacgg tcgcggagga
     1561 gcgggtgcgc acgttctcgt ccggcatgag gcggcgcctc gggctggccc gcgtgctcct
     1621 ggcccaggcc cggctgctgc tgctcgacga gcccttcacg ggcctcgacc ggcgcggccg
     1681 gaagtgggtg aacgagttcc tgcaggcctt caaggcgggc ggcggcaccg cggtgctggc
     1741 gacccacagc ttcggcggcg gcctcggcgt ggccgaccgc atcggcatcc tggggcaggg
     1801 ccggctcctg ctggaccggc cggccgccga tctctcgccc gaggagctgc atcgcgtgta
     1861 cgagagcctc accgagggcg cggaggccgg cgcgtgacct acgcccggcg ggcctggatc
     1921 atcttctaca aggacctcct cgtcgagcgg cgctcgaagg agcagaccaa cgcgctgctc
     1981 ttcttctgcc tgctcctgct gttcctcttc cagttcgcgc tgggcccgga ccgcgaccgc
     2041 ctggccgccg cgctccccgg cctgctctgg ctcggcgtca tcctggccgg cctgctcggt
     2101 ctgggccgga gcttcctggc cgagcgcgag aacgactgct gggagggcct gcggctcacc
     2161 ccgggcgaca agtcggccat ctacctcggc aagctcgcgg gcaactgcgt gctgatgacc
     2221 gccgtcgagg tcgtgctggt ggtactcttc ggcgtgttct acaacgtgga tctctggacg
     2281 ccggcggcgc ccctggccct ggtgctggcc ctgggcacca tcgggctcgc cgcggtgggc
     2341 acgctcttcg ccgccatgac cacgcaggtg cgcgcgcgcg agctgctctt cccggtgctg
     2401 ctgctgccgg tgcaggtacc gctgctgctg gccaccgtga gcgcgacgca ggccctgctg
     2461 ctgggccagc ccctcgccga cgtggcgcac tggctcaagc tgctggccgg ggccgacgcg
     2521 gtctacgtga cgatcgggct gctcaccttc gagttcgtgc tcgagggctg agccgggaga
     2581 ggacatgacg cgcgcgctcg gctggctggc ggcactgggg ctgctggcgg gactcgcggc
     2641 cggcttcggc tacgcgcccc gcgaggccgt gcagggcaac gtgcagcgca tcatgtacct
     2701 gcacgtgccc tcggtgctca ccgcctacct ggccttcggc ctggtgctgc tcggctccat
     2761 cgcctacctg gcgacgcggc gccccggctg ggatcggctc gccggctcgg ccgcggagat
     2821 cggcgtgctc ttcaccgggc tcacgatcgt ctccggctcc atctggggca aacccacctg
     2881 gggcacctgg tggacgtggg acgcgcggct cacctcgacc gcggtgctct tctgcgtcta
     2941 cgtggggtac ctgctgctgc ggagcctcat cgacgatccc gaggcgcgcg gacgctacgc
     3001 cgcggtggtg ggcatcctcg gcgcggtcaa catcccgatc gtgcacttct cggtgaagtg
     3061 gtggcgggcg ctccaccagc cctcgacgat cctcggcccc gagccctcgc cgatcgatcc
     3121 cgcgatcggc ctcgcgctct tcgtgaactg ggtggccttc acgctgctgt tcgcctactt
     3181 cctgtcccgg cggatggaga tcgcccggct cgaggagcag acctgatgcc ggccaactgg
     3241 ggctacgtga tcgccgccta cggcatcgcc gcggtcgcgc tcttcggcta ctggcgccgc
     3301 ctcgcgcgcc gggcccgcgc cctcgccgcg cgaccgcgga ccgcggcgaa gacgagggcc
     3361 gcgtgaaccg caaggccaag ttcatcgggg gcggcctcgt catcgtggcc gcgctcgcct
     3421 acctgatcta cgccggcgtg agccagtcgg tggtctactt cgtcactccg tcggagctgg
     3481 gcgcggcccc cgtcgcgggc aagtcgtacc gcctcggcgg catggtcctg cccggcacgc
     3541 tcaagtggga cgccaggagc ctcgacctgc gcttcacgct ctccgacggc caggcctccg
     3601 tcccggtgcg ccaccggggc acgccgcccg atctgttcgc ggagagccgc ggcgcggtcg
     3661 tggaaggcac ctggagccac gacggctact tccaggccac cacgatcctg gccaagcact
     3721 cggaggagta caaggcgccg catgatcaga gccaggctgg ctacaaggac ctgctcaaga
     3781 ccctgaaagg ctcggaggga aagccatgac gcctgagctg ggctacgccg cgaccgtcgc
     3841 cgccctgatc ctggcggtct acggatccgg cgcggccgcg ctgagcgcgg tccggggacg
     3901 cgcggacctg cgcctctccg cggagcgcgc ggcggttggc gtctggctgc tggtcctgac
     3961 gtgcatggcg ctgctgatct acgccttcct caccttcgac ttctcggtcc gctacgtggc
     4021 cggcaacacc aacctgggca cgcccttcta ctaccgcatc accgcggtgt ggggcgcg
//
LOCUS       DATMCV010000824         4665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_42475, whole genome shotgun sequence.
ACCESSION   DATMCV010000824 DATMCV010000000
VERSION     DATMCV010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42475"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1286)
                     /locus_tag="VNU03_18945"
     CDS             complement(48..1286)
                     /locus_tag="VNU03_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76606.1"
                     /translation="MDTATLPDEVQAVVERLGGADVVVGLATSGPVPALAAVAAAVRT
                     GLDAHCAGQAAAVVHVDQTPSETSTAAVTAALGDLRVVSVPAPGGHGDDGLDWSAAVR
                     AVLTVARRLEARTIVMLNAELSSMTSEWLRGLALPVLKDDYGLMLPLYRRYRYDGTLT
                     QALVVPLMRSLFGRQLAHPLAEEFACSGAAADFFLQQPIWDTELGRQGLEFWLPAAAI
                     EQRLTVGQSVLGPRTVAAAAHPPAPLGRTVGRVAGALFALADRTESFWLDVHGSEPVV
                     SFGVPPEPLPGGPPVDPSLMQVGFVQGVRDLLPVWERILAPENLGQVLELSDRPLERF
                     RFSDRLWTRVVYDFVLAYRTRVVYRSHAAQSLAPLYLGRAASVVLETQGRPPLAMLQS
                     AARLGGMFEEEKPYLVDRWR"
     gene            complement(1286..2485)
                     /locus_tag="VNU03_18950"
     CDS             complement(1286..2485)
                     /locus_tag="VNU03_18950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP76607.1"
                     /translation="MAPGALLKLDDYRQVAPRGTVDFLMRIGERLRGKRLVHVSASRY
                     GALAEGLNRVVAILNDLGVETTWEITIGTADFDQVSRAVAKGLAGTEQVITEAMLGRL
                     RETGVDNARRLRLDADLVMVHDASPLGLVEQRGDAARWVWRCHQDLSSAQPQLWNALR
                     PMVQRYDAAVFSLARFAAPLSIPRFIVYPAIDPLSERNREMTRSEQALQLDRLHVPRD
                     KPILLQVGPFERQHDPIGVINAYRLVKRHHDVRLVLAGPPPAPGGDALGDVQEAASHD
                     PDIAVILLPPDPQTELNALERAATIAIQKPLKADFTLEVASAMWKGKPVIGTIAGGIP
                     FQMVVNVTGYTVETVEGAAFRIRQLLSNPELIGRMGAAGREHVRRNFLVTRHLSDYLA
                     LLAHLTR"
     gene            complement(2485..3147)
                     /locus_tag="VNU03_18955"
     CDS             complement(2485..3147)
                     /locus_tag="VNU03_18955"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HWP76608.1"
                     /translation="MSETKPFVFLGCLDLNELLPHEAHDARELLEQLEKVPVESVFCH
                     TSAALLHRPVLAEAYPNDFSLWVGTEVRDVRLAERLAAVDAFHAGSMERVREDLIATI
                     SDHLQHLGTAPPRTQGKRFRFFQVHLVPVPTGHQARTLDEFRDALAEVDVSALFYHTI
                     EARYRAGRGRGDFAEWVGGVLGRSDLADRLAHIDPHAGSLERIRDRHLSVLSDAISGE
                     MS"
     gene            3177..4379
                     /gene="glgA"
                     /locus_tag="VNU03_18960"
     CDS             3177..4379
                     /gene="glgA"
                     /locus_tag="VNU03_18960"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HWP76609.1"
                     /translation="MRILLLSREYPPHVYGGAGVVVDHLSRALARRATVEVRCFGDQA
                     LTAPGLSARGYAPWDRLAGGPETAYAPALEALSVGLAMARDPVAADVVHTHTWYVGFG
                     GMLVQSIHDLPLVVTLHSMEPLRPWKADQLGTGYAVSSWAERLAVERAERVIAVSAQM
                     RGDILTHFQVDPDRVVVIHNGVDAEAFRRTEQREALARHGVREPYALFVGRISEQKGI
                     FQLLEAARTLPEEVTLVLCASSPDTPELAARLEAAVAGRSRIRWINAMLPVPEVVQLY
                     SHAAVFVCPSIYEPFGLINLEAMACGTPVVASRVGGIPEVVVDGETGWLVPPGDPVAL
                     AQALRGALADPGRARQMGEAGRRRVEAHFSWDRIADRTLAVYQDAIAAKKAARAAAEG
                     GHRADSAR"
     gene            complement(4355..>4665)
                     /locus_tag="VNU03_18965"
     CDS             complement(4355..>4665)
                     /locus_tag="VNU03_18965"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HWP76610.1"
                     /translation="QAWLALHQTLLHLRTTAIVPLLAGEPVPAASWKALGDNALEVTW
                     EFPAGTLRLVTNLGLVPVAHPGPGPDWGRRLHALLLPAAGWRELPPWSAAFYLAESAR
                     "
BASE COUNT          648 a         1645 c         1775 g          597 t
ORIGIN      
        1 caccgcggga cgcacgtgct cgatgacgat ggcgcggcgc gcaggcttca tcgccaccgg
       61 tccacgaggt agggcttctc ctcctcgaac atgccgccca ggcgcgccgc gctctgcagc
      121 atggcgagcg ggggacggcc ctgcgtctcc agaaccaccg acgcggcgcg gccgagatag
      181 agcggggcca gcgactgcgc ggcgtggctc cggtagacga cgcgcgtgcg ataggccagg
      241 acgaagtcgt agaccacccg ggtccacagc cggtccgaga agcggaagcg ctccagcggc
      301 cgatcgctca gctcgagcac ctggccgagg ttctcgggcg ccaggatccg ctcccacacc
      361 gggagcaggt cccgcacgcc ctggacgaag cccacctgca tcagggaggg atcgaccggg
      421 gggccgccgg gcaagggctc gggcggcacg ccgaagctca ccaccggctc cgagccgtgc
      481 acgtcgagcc agaagctctc ggtgcggtcg gcgagcgcga agagcgcgcc ggccacgcgg
      541 cccaccgtgc ggccgagggg cgcgggggga tgggcggcgg cggccaccgt gcgcggcccc
      601 agcaccgact ggcccacggt gagcctctgc tcgatggccg ccgcgggcag ccagaactcg
      661 agaccctgtc gccccagctc ggtgtcccag atcggctgct gcaggaagaa gtcggccgcc
      721 gcgccggagc aggcgaactc ctcggccagc gggtgcgcga gctggcggcc gaacaggctc
      781 cgcatcaggg ggaccacgag ggcctgggtg agggtaccgt cgtagcgata gcgccggtag
      841 agcggcagca tgaggccgta gtcgtccttg agcaccggga gcgcgagccc gcgcagccac
      901 tcgctggtca tgctgctcag ctcggcgttc agcatcacga tcgtgcgcgc ctcgagccgg
      961 cgggccaccg tgagcaccgc ccgcaccgcg gcggaccagt cgagcccgtc gtcgccgtgg
     1021 ccgcccggag ccggcaccga caccacccgg aggtcgccga gggccgcggt caccgcggcg
     1081 gtgctcgtct ccgacggcgt ctggtcgacg tggacgaccg cggcggcctg gcccgcgcag
     1141 tgcgcgtcga gcccggtgcg caccgccgcc gcgaccgcgg ccagcgccgg gaccggaccc
     1201 gaggtggcca ggccgaccac gacgtcggcg ccgccgagcc gctcgacgac cgcctggacc
     1261 tcgtcgggca gggtcgcggt gtccatcacc gggtgaggtg ggcgagcagg gccaggtagt
     1321 cgcttagatg acgggtcacc aggaagttcc ggcgcacgtg ctcgcgcccc gccgcgccca
     1381 tgcggccgat cagctcgggg ttggacagca gctggcggat gcgaaatgcg gcgccctcca
     1441 cagtttccac ggtatacccg gtgacgttga cgaccatctg aaatgggatg ccgcccgcga
     1501 tggtgccgat caccggcttg cccttccaca tcgcgctcgc cacctccagg gtgaaatcgg
     1561 ccttgagcgg cttctggatg gcgatcgtgg cggcccgctc gagcgcgttc agctcggtct
     1621 ggggatccgg cgggagcagg atcaccgcga tgtcggggtc gtgggaggcg gcctcctgca
     1681 cgtcgccgag ggcgtcgccc cccggcgcgg gtgggggccc ggccaggacc aggcggacgt
     1741 cgtggtgccg cttcaccagc cggtaggcgt tgatcacccc gatcggatcg tgctggcgct
     1801 cgaacgggcc cacctggagc aggatcggct tgtcgcgcgg cacgtgcagc cggtccagct
     1861 ggagcgcctg ctcgctgcgg gtcatctcgc ggttgcgctc cgagagcggg tcgatcgcgg
     1921 ggtagacgat gaagcgcggg atggacagcg gcgccgcgaa gcgggccagg gagaagaccg
     1981 cggcgtcgta gcgctgcacc atcggccgca gcgcgttcca gagctggggc tgggccgacg
     2041 agagatcttg gtgacagcgc cacacccagc gggccgcgtc cccgcgctgc tcgaccagcc
     2101 ccaacggcga cgcgtcgtgg accatcacca gatccgcgtc gagccgtagg cggcgggcgt
     2161 tgtcgacccc ggtctcgcgc aggcggccga gcatggcctc ggtgatgacc tgctcggtgc
     2221 ccgcgaggcc cttggccacc gcgcggctca cctggtcgaa gtccgcggtg ccgatggtga
     2281 tctcccaggt ggtctcgacg cccaggtcgt tcaggatggc caccacccgg ttgagaccct
     2341 cggcgagcgc gccgtagcgc gaggcgctca cgtggacgag ccgcttgccg cggaggcgct
     2401 cgccgatgcg catgaggaag tccaccgtgc cgcggggggc cacctggcgg tagtcgtcga
     2461 gcttgagcag cgcgccgggc gccatcagga catctccccg gagatggcgt cgctcagcac
     2521 gctcaggtgg cggtcgcgga tgcgctcgag gctgcccgcg tgcggatcga tgtgggccag
     2581 gcgatcggcc agatcggacc ggccgaggac gccgccgacc cactccgcga aatcgccccg
     2641 gccgcggccc gcgcggtagc gcgcctcgat cgtgtgatag aagagcgcgc tcacgtccac
     2701 ctccgcgagg gcgtcgcgga actcgtccag ggtgcgggcc tggtggccgg tgggcaccgg
     2761 caccaggtgg acctggaaga agcggaagcg cttgccctgg gtgcgcggcg gggccgtgcc
     2821 gaggtgctgg aggtgatcgg agatggtggc gatcagatct tcgcgcaccc gctccatgga
     2881 ccctgcgtgg aaggcgtcga ccgcggcgag ccgctcggcg agccgcacgt cgcgcacttc
     2941 ggtgccgacc cagagcgaga agtcgttggg gtaggcctcg gcgagcaccg ggcggtggag
     3001 cagggccgcc gaggtgtggc agaagaccga ctccaccggc accttctcga gctgctcgag
     3061 cagctcgcgg gcgtcgtggg cctcgtgggg caggagctcg ttcaggtcga ggcagccgag
     3121 gaagacgaag ggcttggtct cgctcatgcg gtaagctctc cggaaggacg gtgcgagtga
     3181 gaatcctgct cctgagccgc gagtacccgc cgcacgtcta cggcggcgcc ggcgtggtgg
     3241 tggaccacct gagccgcgcg ctggcccgcc gcgccaccgt cgaggtgcgc tgcttcggcg
     3301 accaggccct gaccgcgccg ggcctgagcg cgcggggcta cgctccgtgg gaccggctgg
     3361 ccgggggacc ggagacggcc tacgctcccg cgctggaggc gctctcggtc gggctcgcga
     3421 tggcccgcga tccggtcgcc gccgacgtgg tccacaccca cacctggtac gtgggattcg
     3481 gcggcatgct cgtgcagtcg atccacgacc tgccgctcgt ggtcacgctc cacagcatgg
     3541 agccgctgcg accgtggaag gccgatcagc tcggcaccgg ctacgcggtg tcgagctggg
     3601 cggagcggct ggccgtcgag cgcgcggagc gcgtgatcgc ggtgtcggcc cagatgcgcg
     3661 gcgacatcct gacccacttc caggtcgacc cggaccgcgt ggtggtgatc cacaacggcg
     3721 tcgacgcgga ggccttccgg cgcaccgagc agcgggaggc cctcgcgcgc cacggggtgc
     3781 gcgagcccta cgcgctcttc gtcggccgca tcagcgagca gaaggggatc ttccagctgc
     3841 tggaggcggc ccggaccctg cccgaggagg tcacgctcgt gctctgcgcg agcagccccg
     3901 acaccccgga gctggccgcc cggctcgagg ccgcggtggc cggccgctct cgcatccgct
     3961 ggatcaacgc gatgctcccg gtcccggagg tcgtgcagct ctacagccac gccgcggtgt
     4021 tcgtgtgccc gtcgatctac gagccgttcg gcctcatcaa cctggaggcg atggcgtgcg
     4081 gcacgccggt ggtggcgagc cgggtgggcg gcatccccga ggtagtggtc gacggcgaga
     4141 ccggctggct cgtcccgccg ggcgatcccg tcgcgctcgc ccaggccctc cgcggggcgc
     4201 tcgccgatcc ggggcgcgcc cgtcagatgg gcgaggccgg ccgccggcgc gtcgaggcgc
     4261 acttctcctg ggatcggatc gcggaccgta cgctcgcggt ctaccaggac gcgatcgcgg
     4321 ccaagaaggc cgcccgcgcg gccgccgagg gcggtcaccg cgccgactcc gcgaggtaga
     4381 aggccgcgct ccacggcggc agctcgcgcc acccggccgc gggcaggagg agcgcgtgga
     4441 gccgccggcc ccagtcgggg ccgggccccg gatgcgccac cgggaccaac cccaggttgg
     4501 tcaccaggcg cagcgtcccg gccgggaact cccacgtgac ctcgagggcg ttgtccccca
     4561 gggccttcca cgaggcggcc ggcaccggct cgccggcgag cagcggcacg atggcggtcg
     4621 tgcgcaggtg gaggagggtt tggtggagcg cgagccaggc ctggt
//
LOCUS       DATMCV010000825         3109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_510497, whole genome shotgun sequence.
ACCESSION   DATMCV010000825 DATMCV010000000
VERSION     DATMCV010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510497"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VNU03_18970"
     CDS             <1..850
                     /locus_tag="VNU03_18970"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP76611.1"
                     /translation="EVVARALETRRKDVILVTKCGVRVPPPGQPGPLRDASRANILLE
                     VDASLKRLRTDWVDVLLVHWPDASTPFEDTMRALDEVVASGRARFVGVSNFTGAMLAE
                     CQRTRRVDVSQVGYHMLDRRQEQETFPLCLREGIGVMGYGSLGHGLLTGAFTSATAFE
                     AGRDWRAGGVAFGQPIFRGDNLKTNVGVVERIRREIAEPRGVAVSQIALAWVLANPAI
                     STALVGARTPAEVDANDAGAELELTVAERAALDAILEGAAGRVREFTPLRPAMEPWGE
                     ELPAGR"
     gene            complement(854..2398)
                     /locus_tag="VNU03_18975"
     CDS             complement(854..2398)
                     /locus_tag="VNU03_18975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76612.1"
                     /translation="MRNWRIAVVLMGIALGPVAAEAAPEGRVVIAQGVDPTTLDMMHQ
                     QEQPASNVGAQIFESLVERGPDLKIVPALATEMPKLVAPTTWEIKLRRGVKFHNGEDF
                     NAESVKFSLERLANPANKLRGSTSFAPIDRVEIVDPFAVKVHTKKPWPVFVVHLALRQ
                     AAMYPPKEYAGKDPAAISRNPIGTGPYKFVRWSKDEEIVMEAFPGYWAGAAKIKTIVF
                     KPIPDDAVRVAALQNGEIDVAVNIPPHLAGIIEKHPKIYLSTAPSIRTIQLMIYTHQM
                     DANHKPTGPYAGPTADKRVRQALAYAIDADEIIKGVMDGKAVRVATMLPSLHFGYDAS
                     LKPIKQDLARSKKLLAEAGFPNGVEIVLNGPQGRYVRDKEVGEAVTGQLNKAGIKTTL
                     RTYEFVNYLNNMVYVHKAGPVWLIGWGHPTMDAEAIYVPLFKSPGIFVNWQNEDFNGM
                     VEQAQSQMDEKKRYAEYQKINKLWIEEVPAIPLYQQIDLYGANKRLNWKARSDELIRA
                     YDMSLR"
     gene            complement(2466..>3109)
                     /locus_tag="VNU03_18980"
     CDS             complement(2466..>3109)
                     /locus_tag="VNU03_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP76613.1"
                     /translation="DDEGKKVEAGIRAGGGDAIYLHLDVTSEADWRAAVQTALDSHGR
                     LDVLVNNAAIVIPKVPIEERTVAEWDQVMAVNAKGVFLGTKYAIPAMRRSGGGSIVNI
                     SSVAGIGQSLHQEPAYAASKGAIRIFTKVTASQHAKDKIRCNSVHPGPVDTEMFHSAF
                     RDRDAMEKRLQRVPLRRMGTVPEIVSAVLYLASDDSSYMTGSELVIDGGALAQ"
BASE COUNT          418 a         1069 c         1081 g          541 t
ORIGIN      
        1 ggaagtggtc gcccgcgcgc tcgagacccg gcgcaaggac gtgatcctgg tcaccaagtg
       61 cggggtgagg gtccccccgc cgggccagcc cgggccgctg cgggacgcga gccgggccaa
      121 catcctgctc gaggtcgacg cgagcctgaa gcgcctgcgc accgactggg tggacgtgct
      181 gctcgtgcac tggcccgacg cgtccacccc gttcgaggac acgatgcgcg ccctcgacga
      241 ggtggtggcg tccggccgcg cgcgcttcgt cggcgtctcc aacttcaccg gcgccatgct
      301 ggccgagtgc cagcgcaccc gacgggtcga cgtctcgcag gtgggctacc acatgctcga
      361 ccgccgtcag gagcaggaga ccttcccgct ctgcctgcgc gaaggcatcg gggtgatggg
      421 ctacggctcg ctcggccacg ggctcctgac cggggccttc acctccgcca ccgcgttcga
      481 ggcggggcgc gactggcgcg cgggcggcgt cgcgttcggg cagccgatct tccgcggtga
      541 caacctcaag accaacgtcg gcgtggtgga gcggatccgg cgggagatcg cggagccgcg
      601 cggggtcgcg gtgagccaga tcgcgctcgc gtgggtgctc gccaatcccg cgatcagcac
      661 cgcgctggtg ggcgcgcgca cccccgccga ggtggacgcc aacgacgcgg gggccgagct
      721 cgagctgacc gtggccgagc gcgccgcgct cgacgcgatc ctcgaggggg cggccggccg
      781 cgtgcgggag ttcaccccgc tgcggccggc gatggagccg tggggcgagg agctccccgc
      841 ggggcgctag ccgctagcgc agcgacatgt cgtaggcgcg gatcagctcg tcgctgcggg
      901 ccttccagtt gagccgcttg ttggccccgt agagatcgat ctgctggtac agcgggatcg
      961 cggggacctc ctcgatccag agcttgttga tcttctggta ctccgcgtag cgcttcttct
     1021 cgtccatctg cgactgcgcc tgctccacca tcccgttgaa gtcctcgttc tgccagttca
     1081 cgaagatccc gggcgacttg aagagcggca cgtagatcgc ctccgcgtcc atcgtcggat
     1141 ggccccagcc gatgagccac accggacccg ccttgtgcac gtagaccatg ttgttcaggt
     1201 agttcacgaa ctcgtaggtc cgcagcgtgg tcttgatccc ggccttgttg agctggccgg
     1261 tgaccgcctc cccgacctcc ttgtcgcgga cgtagcggcc ctgcgggccg ttcagcacga
     1321 tctcgacccc gttggggaag ccggcctccg cgaggagctt cttgctgcgg gccaggtcct
     1381 gcttgatcgg cttgagcgag gcgtcgtagc cgaagtgcag gctcggcagc atggtggcca
     1441 cccgcaccgc cttgccgtcc atcaccccct tgatgatctc gtccgcgtcg atcgcgtagg
     1501 cgagggcctg gcgcacgcgc ttgtccgcgg tcgggcccgc gtaggggccg gtgggcttgt
     1561 ggttggcgtc catctggtgc gtgtagatca tgagctggat cgtgcggatg gacggcgcgg
     1621 tgctgaggta gatcttgggg tgcttctcga tgatgccggc caggtggggc gggatgttca
     1681 ccgccacgtc gatctcgccg ttctggagcg cggccacccg caccgcgtcg tccgggatcg
     1741 gcttgaacac gatggtcttg atcttggccg ccccggccca gtagcccggg aaggcctcca
     1801 tcacgatctc ctcgtccttc gaccagcgga cgaacttgta cggtccggtg ccgatcgggt
     1861 tccgggagat cgcggccggg tccttgcccg cgtactcctt ggggggatac atggcggcct
     1921 ggcgcagggc caggtggacc acgaagaccg gccacggctt cttggtgtgg accttcaccg
     1981 cgaacggatc gacgatctcc acccggtcga tcggcgcgaa cgacgtcgag ccgcgcagct
     2041 tgttggccgg attggccagg cgctcgaggc tgaacttgac cgactccgcg ttgaagtcct
     2101 ccccgttgtg gaacttgacc ccgcggcgca gcttgatctc ccaggtcgtc ggcgccacca
     2161 gcttcggcat ctcggtggcc agggcgggca cgatcttcag atcggggccg cgctccacca
     2221 gcgactcgaa gatctgcgcg cccacgttgg aggcgggctg ctcctgctgg tgcatcatgt
     2281 cgagcgtggt cggatccacg ccctgcgcga tcacgacccg gccctcgggc gccgcttccg
     2341 ccgccaccgg gcccagcgcg atgcccatca gcaccaccgc gatccgccag ttcctcatgc
     2401 gcaccacctc catggggggt caggtcttac attacgacat tcggaatatt gctggattac
     2461 cggtcctact gggccagcgc gccgccgtcg atgaccagct cactgccggt catgtaggag
     2521 gagtcgtcgg aggcgaggta gagcaccgcg ctcacgatct cggggacggt gcccatccgg
     2581 cgcaggggca cgcgctggag gcgcttctcc atggcgtcgc ggtcgcggaa ggcgctgtgg
     2641 aacatctcgg tgtcgaccgg gcccgggtgg accgagttgc agcggatctt gtccttggcg
     2701 tgctgggacg cggtcacctt ggtgaagatc cggatcgcgc ccttgctcgc cgcgtaggcc
     2761 ggctcctggt gcagcgactg gccgatgccg gccaccgagg agatgttgac gatcgagccg
     2821 ccgcccgagc ggcgcatggc cgggatcgcg tacttggtgc cgaggaagac gcccttggcg
     2881 ttcaccgcca tcacttggtc ccactccgcg acggtgcgct cctcgatcgg caccttgggg
     2941 atgacgatgg ccgcgttgtt gaccaggacg tcgagccgcc cgtggctatc gagcgcggtc
     3001 tgcaccgccg cccgccagtc cgcctcgctg gtcacgtcca ggtggaggta gatggcgtcg
     3061 ccgccccccg cccggatgcc cgcctcgacc ttcttcccct cgtcgtccc
//
LOCUS       DATMCV010000826         5150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_840111, whole genome shotgun sequence.
ACCESSION   DATMCV010000826 DATMCV010000000
VERSION     DATMCV010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840111"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VNU03_18985"
     CDS             complement(<1..658)
                     /locus_tag="VNU03_18985"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76614.1"
                     /translation="MRILRVIVAVAVLSFVAVQLGPVRAAGPVSEVLIGELLPLTGNF
                     ASLGQQYLWAGQTVEDIVNNDYPDLQVPLGPGKGFPGLGGAKMTLVVRDDQSRGDQAR
                     TLAEQLITVNKVHWLNGEGTSGNTSIIQPLVESYGIPMSCHPCASPTLTEKGFKWFYR
                     TGPNDGTMVASVFQFLKEWPSHGGPSDLKTIAMFTCDNLFCQDNRKIALDLSEKGGYK
                     I"
     gene            complement(897..1961)
                     /locus_tag="VNU03_18990"
     CDS             complement(897..1961)
                     /locus_tag="VNU03_18990"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP76615.1"
                     /translation="MRFSICVMADVDEIGFFSHAEALDYDSVWVTDSQMLFSDCYAVL
                     ALAAQQTRRIRLGPGVAICGTRIPPVHVAAMATLNRLAPGRVHLGVGTGNTATRSMGQ
                     PPMRIAEYAEHLRVLRGLLRGEPVDYTQDGSTRPIKMLIREKKYMSLEPRIPLYVSAF
                     GPRAMELAGEYGNGVVFAIPPRGIAPAEALARCRVGATRAGRSLDDFHTCALTNVVLL
                     DPGEPADSERVIRTVGPNVMASVYYFYDAVHERGIEVPPFLRRIWSRYCTLVEEVPRP
                     HRHFRTHEGHYTYLHPGEAALIDAELVRATCLVGEADELTERIRELERGGLRELMFAT
                     GVDEKWRFSQEFARRVMARY"
     gene            complement(1974..2720)
                     /locus_tag="VNU03_18995"
     CDS             complement(1974..2720)
                     /locus_tag="VNU03_18995"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP76616.1"
                     /translation="MKLLLSGLAVVLLVAAPVSAPGQTPVETARGLLKQYQEDPTRID
                     RARDLLEAAVAKGAGSDVPTLVTLARAWFLYGEERATTDEAKVAAYERARDLAKRATE
                     LAPRDPDAHFLYAISLGSWSQAKGLLRSALALRDLRREVDRVLELDPTNIGAHIMAGS
                     IARELPVLLGGDRKDAEAHFKTARHLDPRLTGARIELAQLYVNMGRYAEARQELQAVL
                     TEKAPTDRPRWTLKEVPRARQMLESIRDKN"
     gene            2853..3332
                     /locus_tag="VNU03_19000"
     CDS             2853..3332
                     /locus_tag="VNU03_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76617.1"
                     /translation="MTLGMTLETFTRLHVVISLIGIASGLMAVFGMMSGVRRDGWTTL
                     FLWTTVLTSVTGFGFPVEHLLPSHKVGIISLVVLAIVFVARSRAHSGSWRWIYVIGSV
                     LALYLNVFVLVVQLFQKVPVLAVLAPTQSELPFQLAQLAVFALFVWFAIAATRRFRF"
     gene            complement(3329..4588)
                     /locus_tag="VNU03_19005"
     CDS             complement(3329..4588)
                     /locus_tag="VNU03_19005"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76618.1"
                     /translation="MRHLATWSLIALLASACGVSRTEPPPTSQETVIALVGGRVQTAP
                     EAMPIPDGVVVLRNDVITAVGRRADVRVPPEATVIDCAGGTVTAGFWNSHVHFTQPTW
                     SGAATAPAERLTTALRGMLTSYGVVRALDTGSEQANTEALRRRIASGEVLGPTIMMAA
                     GSIAPVGGSPYYILPARLPEAESAAAVVAIVETALDRGAEGIKLFTGSWATPRSIVVM
                     PVEMVRAATDAAHRRGRFVIAHPSNSAGARAAIEGGVDILAHTFPASPDRQPWDRALP
                     GMMRERGMALIPTLKLWPYELAKAGAPAEVTRLVLGNGQAQLKAFIDLGGQVLFGTDV
                     GYMMEYDPTDEYVYMQQAGLSYAQILAALTTAPAARFGGASRTGRLTPGFAADVVVLD
                     GDPAEDIRALARVRATLRAGRIIYRRP"
BASE COUNT          733 a         1759 c         1850 g          808 t
ORIGIN      
        1 cgatcttgta gccgcccttc tcggacagat cgagcgcgat cttgcggttg tcctggcaga
       61 agaggttgtc gcaggtgaac atcgcgatcg tcttgaggtc cgacgggccg ccgtgcgacg
      121 gccactcctt gaggaactgg aagacgctcg ccaccatggt gccgtcgttg ggcccggtgc
      181 ggtagaacca cttgaagccc ttctcggtca gcgtcggcga ggcgcaggga tggcagctca
      241 tcgggatgcc atagctctcc accagcggct ggatgatcga ggtgttgccg ctcgtgccct
      301 cgccgttcag ccagtgcacc ttgttgaccg tgatgagctg ctcggccagg gtgcgcgcct
      361 gatcgccgcg gctctggtcg tcgcgcacca ccagcgtcat cttggccccg cccaggccgg
      421 ggaagccctt gccggggccg aggggcacct gcagatccgg gtagtcgttg ttgacgatgt
      481 cctcgacggt ctgcccggcc cagaggtact gctggcccag gctcgcgaag ttcccggtga
      541 gcggcagcag ctccccgatc aggacctcac tgaccgggcc ggcggcccgg acgggcccga
      601 gctgcaccgc gacgaacgac agcaccgcga ccgcaacgat gacgcggaga atccgcatgc
      661 gtgcccctcc ttacagtgag tgcgggaaac ggccggctcg cgaccatggc gggccgcgac
      721 cggaaccgcc ggccggcggg cccgacttgc gcgcgccgag tctagcgcag gacccccgct
      781 ctgagaaggg cccttattcc tgaagggccc ttattcctct tcccctctcc cctcagagga
      841 gagggcaggg tgaggggtgg tcagggtgag gggtagagag tgaggggtag agcgacctag
      901 taccgcgcca tcacccgccg ggcgaactcc tggctgaagc gccacttctc gtccaccccg
      961 gtggcgaaca tcagctcccg gagcccgccg cgctcgagct cgcggatgcg ctcggtcagc
     1021 tcgtcggcct cgcccaccag gcaggtggcg cgcaccagct ccgcgtcgat cagcgcggcc
     1081 tcgccggggt gcaggtaggt gtagtgcccc tcgtgggtgc ggaagtgccg gtgcggccgc
     1141 ggcacctcct ccaccagcgt gcagtagcgc gaccagatgc gccgcaggaa gggcggcacc
     1201 tcgatgccgc gctcgtgcac cgcatcgtag aagtagtaga cgctggccat cacgttcggc
     1261 cccaccgtgc ggatgacccg ctcggagtcg gccggctcgc ccgggtcgag caggaccacg
     1321 ttggtgagcg cgcaggtgtg gaagtcgtcc aggctccggc cggcccgggt ggcgccgacg
     1381 cggcagcggg cgagggcctc cgcgggcgcg atgccgcgcg gcgggatcgc gaagacgacc
     1441 ccgttgccgt actcgccggc cagctccatg gcccgtgggc cgaacgcgga gacgtagagg
     1501 gggatgcgcg gctcgaggct catgtacttc ttctcccgga tcagcatctt gatcgggcga
     1561 gtgctcccgt cctgcgtgta gtccaccggc tccccccgca acaggccgcg cagcacccgc
     1621 aggtgctccg cgtactccgc gatgcgcatg ggcggctgcc ccatcgagcg ggtggcggtg
     1681 ttgccggtgc cgacgcccag gtggacgcgg ccgggggcga ggcggttcag ggtcgccatc
     1741 gcggccacgt gcaccggcgg gatgcgggtg ccgcagatgg cgaccccggg gcccaggcgg
     1801 atgcggcgcg tctgctgcgc cgcgagggcc agcaccgcgt agcagtcgga gaagagcatc
     1861 tggctgtcgg tgacccagac cgagtcgtag tccagggcct cggcgtggct gaagaagccg
     1921 atctcgtcca cgtcggccat cacgcagatg gagaagcgca tgcgcgtcct ttctcagttc
     1981 ttgtcgcgga tggactcgag catctggcgg gcccgcggca cttccttgag ggtccagcgc
     2041 ggtcggtcgg tgggggcctt ctcggtcagc accgcctgca gttcctggcg tgcctccgcg
     2101 tagcggccca tgttcacgta gagctgggcc agctcgatgc gcgccccggt gagccggggg
     2161 tccaggtgcc gggcggtctt gaagtgcgcc tcggcgtcct tgcgatcgcc gcccagcagc
     2221 accggcagct cgcgcgcgat gctcccggcc atgatgtgcg ccccgatgtt cgtcgggtcc
     2281 agctcgagga cccggtccac ctcgcgccgc aggtcgcgca gggccagggc cgagcggagc
     2341 aggcccttgg cctgcgacca ggagccgagg ctgatcgcgt acaagaaatg cgcgtccggg
     2401 tcgcgggggg ccagctcggt cgcgcgcttg gcgagatcgc gcgcccgctc gtaggccgcg
     2461 accttggcct cgtcggtggt ggcccgctcc tcgccgtaga gaaaccaggc gcgggccagg
     2521 gtcacgaggg tcggcacgtc gctgcccgcc cccttggcga ccgcggcctc gagcagatcg
     2581 cgcgcgcggt cgatgcgggt ggggtcctcc tggtattgct tcaggaggcc gcgcgcggtc
     2641 tccaccgggg tctggcccgg tgccgacacg ggggcggcca cgagcaggac cacggcgagg
     2701 ccggatagga ggagtttcac gggccgatca acgctggccg gatgatagca cccggcaccg
     2761 cggcccggcg gccggtagcc caggccccgg ggttcggcta cgatggcccg ggtgcccgga
     2821 ctctcggacg acgaaaggag acgagaccga ccatgactct ggggatgacg ctggagacgt
     2881 tcacccggct gcacgtcgtg atcagcctga tcggcatcgc ctcgggcttg atggccgtgt
     2941 tcgggatgat gtccggggtg cggcgcgacg gctggacgac cctcttcctg tggaccaccg
     3001 tgctgacgag cgtgaccggg ttcggcttcc cggtcgagca tctgctgccg tcgcacaagg
     3061 tcgggatcat ctcgctggtc gtgctggcca tcgtgttcgt ggcgcgctcc cgggctcaca
     3121 gcggctcgtg gcgctggatc tacgtgatcg gctcggtgct cgccctgtac ctgaacgtgt
     3181 tcgtgctcgt ggtgcagctc ttccagaagg tccccgtcct ggccgtgctg gccccgaccc
     3241 agtccgagct gccgttccag ctcgcgcagc tcgcggtctt cgcgctcttc gtgtggtttg
     3301 cgatcgcggc gacgcggcgg tttcgcttct agggtcgccg gtagatgatc cggcccgcgc
     3361 ggagcgtcgc gcgcacgcgg gcgagggcgc ggatgtcctc ggcgggatcg ccgtccagca
     3421 cgacgacgtc cgccgcgaac cccggggtga ggcgcccggt ccggctcgcg ccgccgaagc
     3481 gcgccgccgg cgcggtcgtg agcgcggcga ggatctgggc atacgacagg cccgcctgct
     3541 gcatgtagac gtactcatcc gtcgggtcgt actccatcat gtagccgacg tcggtgccga
     3601 acaggacctg cccacccagg tcgatgaatg ccttgagctg ggcctgaccg tttccgagga
     3661 cgagccgggt cacctccgcg ggcgccccgg ccttcgcgag ctcgtagggc cagagcttca
     3721 gcgtcggaat gagggccatg ccccgctccc gcatcatccc gggcagggcg cggtcccacg
     3781 gttgacggtc cgggctggcg gggaaggtgt gcgcgaggat gtcgacgccg ccctcgatgg
     3841 cggcccgcgc tcccgcgctg ttggacgggt gcgcgatgac gaaccggccc cggcgatgcg
     3901 ccgcgtccgt ggcggcgcgc accatctcga ccggcatgac cacgatcgag cggggcgtgg
     3961 cccacgagcc ggtgaagagc ttgatgcctt ccgcgccccg gtcgagcgcg gtctccacga
     4021 tcgccaccac cgcggcggca ctctcggcct ctgggagtcg ggcgggcagg atgtagtaag
     4081 ggctcccgcc caccggcgcg atgctcccgg ccgccatcat gatcgtcgga ccgaggacct
     4141 ccccgctcgc gatgcgacgg cgcagcgcct cggtgttggc ctgctccgag cccgtatcga
     4201 gcgcccgcac gacgccgtac gacgtcagca tcccccgcag cgccgtggtg aggcgctcgg
     4261 ccggcgcggt cgcggcgccg ctccaggtcg gctgggtgaa gtgcacgtgg ctgttccaga
     4321 acccggcggt gaccgtgccg ccggcgcagt cgatcacggt tgcctccggc ggcacgcgga
     4381 cgtccgcgcg gcggcccact gcagtgatga cgtcattgcg gaggaccacg acaccatccg
     4441 gtatcggcat cgcctcgggc gccgtctgca cgcgcccgcc caccagcgcg atgacggtct
     4501 cctgggaggt tggcgggggc tcggtcctcg agaccccgca ggccgaagcg agcagtgcga
     4561 tcagcgacca cgtggcgaga tgtctcatgg gaacggtcct taaatgcgcc agagaaggcc
     4621 tgcgagccgg actgtagacg acccaagcat acaggcgcat gaacgctgac gaaacgccct
     4681 cctgggttcg cggtcaggag ccgcggccta taagtgcgtg tagcccccgg agacggtccg
     4741 aggggacaga gcctgcctac tgtggtcgct cgcctagagc cgagaacaga ctcgctgacc
     4801 cgcgagagca gtcgaggggt gcccgggcat ggtctcgacc tctatgaccc gccagcagcc
     4861 cggctggtgg ccgagggaac tattccgtgc agcccttgac acccgctgac cggttctacg
     4921 cggcctatca gcgaaaggag tttcatggcc gatacgtctc tccttctggc ccgccagggc
     4981 gcggtcaggc ttcatagccc gccttggcgg cttggtacgt tggatcgaac cctcgtaggt
     5041 agcctgtagt ctcgaatcgg aatccctagt gggctcacga atggaatcgc gtctgacggg
     5101 aagacggcac acccggctga gacgagactg tcctccgagg gcacgggaac
//
LOCUS       DATMCV010000827         8330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_579526, whole genome shotgun sequence.
ACCESSION   DATMCV010000827 DATMCV010000000
VERSION     DATMCV010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579526"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VNU03_19010"
     CDS             <1..211
                     /locus_tag="VNU03_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76619.1"
                     /translation="ALIRAGAGVELVGPDLFVPLGHGRPHLRRVLSALALYEPAAPGS
                     ATAAPVDGEPSVGPRRLREIRVPLA"
     gene            213..2264
                     /locus_tag="VNU03_19015"
     CDS             213..2264
                     /locus_tag="VNU03_19015"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWP76620.1"
                     /translation="MSALLALRLVTYLLVGTGVAALYGAALIGPVGAGLVVLAILVGW
                     GHEQARARGAVRPALGYCLVGTAAVAITVDLFYLAYSLLDGMVHLLLFLILLRLFVRR
                     GLRDLRDAALLSFFMLVAAASISFSLSFLVVYVVFLVLGTWMLILNHVVTELEQAGRE
                     GAAGSSTRRVFFRGPLTRVSLVAAAITFALAGGLFFIIPRVGQAALPLRAQLGRMVTG
                     FSDRVDLGSFGDIESDPSVVMRVYLTDERVEPATLPELRWRGVAFDQFDGSAWSSAGA
                     TRRYIRRGAAGDFGIGLPRGPGPVLRQEIFLEPMGTDAVFGAPRMLRLELQSPTLVAD
                     DMGGLSTSSATARLHYIVESELEEPTRPAGLAARSGLPAAERARYLQLPELSPDVARL
                     AREATAGTRDPETAARRISAFLSTTYRYTLALRRETTRPPLEEFLFVRRSGNCEYFAA
                     AMAVMLRSEGIPSRVVAGFQHGEWNPYGRYFMVRLSDAHSWVEAYIDGRGWVAFDPSP
                     RGEVASVPPGSVSLYLDAARMRWYRYVINWSLGDQVHLAASVHRQATDVRVGLSWPRH
                     WRVSPAVLAAGGAVVVLALAWLFGRRGFAKGGAKATASVPPFYQRALRLLARRGLSPG
                     AAETARQFATRVGVTAPERAEPFARLTRHYERARFGATALSETEWQDVTLALDTLAAA
                     R"
     gene            complement(2171..2698)
                     /locus_tag="VNU03_19020"
     CDS             complement(2171..2698)
                     /locus_tag="VNU03_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76621.1"
                     /translation="MSMRVAFYKVDGGRLCAWTALIPKRRPLQGTTMAAGRDLPHDLA
                     QFVVESTLGLRCGFWGLLSIGATFESVRRRPTKPGRRVIRTHHAALVATERVVNAHAA
                     AWRAKTATPVGPALDEILARWRSLRAGEELRVEWTISPAAGRAQRAAASVSSASVTSC
                     HSVSLSAVAPKRARS"
     gene            complement(2711..3154)
                     /locus_tag="VNU03_19025"
     CDS             complement(2711..3154)
                     /locus_tag="VNU03_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWP76622.1"
                     /translation="MSEAWRANLVTDQESIARIVRECKRIAVLGIKTEAQRDQPGFYV
                     AEYMASAGYDVVPVPVYFPEATRILDRPVYRTVREVPGPVDMVDVFRRPRDIPPHVDD
                     ILAAHPRVVWLQLGIRHEQAAETFARAGIQVVQDRCLLVEHRRLR"
     gene            3202..3810
                     /locus_tag="VNU03_19030"
     CDS             3202..3810
                     /locus_tag="VNU03_19030"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWP76623.1"
                     /translation="MPVRITRVYTRTGDHGETALVGGRRVPKDSPRIEAYGTIDELNA
                     IIGLARVFNAERKKSGKRHRWLDEVLRRLQNELFDLGSELATPADATYEGMFRVSAEQ
                     VTALERLMDECQKELTPLKSFILPGGGPVGGFLHQARTVCRRAERRVLELSRVEAIGE
                     WPLKYVNRLSDLLFVLSRWVGKHLGETEYLWQRGLDAHARKR"
     gene            3811..4296
                     /locus_tag="VNU03_19035"
     CDS             3811..4296
                     /locus_tag="VNU03_19035"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP76624.1"
                     /translation="MAEDYQIRRAGPADHAGVTRELAEYFAFLGEDVDASGLDHDVAD
                     WQGEYDGAAGVMLIVVDPAGVVVGTAGVRRLAPGVAELKRMWLRPACRGRGLADRLMA
                     RVLEEARALGGATLRLDSESRLEAAVRLYRRYGFREIADYNGNPRADVWMELPLAPGR
                     S"
     gene            4343..5917
                     /gene="ggt"
                     /locus_tag="VNU03_19040"
     CDS             4343..5917
                     /gene="ggt"
                     /locus_tag="VNU03_19040"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWP76625.1"
                     /translation="MVTSPHSLASRAGVQVLQSGGSVVDAAIAAAAVLAVVYPHMTSV
                     GGDAFWLIHEARTGSVHSLDAAGGAAASGTLDWFRARGLGQIPLRGVVPATLTVPGAV
                     DGWCEAHARHGKLPLADDLAAAVRYARDGFPITRRISEWIERSADVLTADPPAARIFL
                     AEGRRPAVGRRLVNPDLARTLERIGIGRAGFYEGETAREMARWAREHGGFFTEHDLAG
                     QRARWGESIRATYRGVTIHETPPPSQGISVLQALRLVESHDLGALPYLGPDHVHLLVQ
                     AKQIAFHDRDRFVADPAFVPVPVERLLSTAYADERRRLIDPARALPWDRVPSEGSLAG
                     DTVYVAAVDEAGNAVSLIQSLYFGFGAGVVAGDTGIVLQNRSAYFSLDPDHPNRLEPG
                     KRPLHTLIASLAFRDGRLWQVVGCMGADGQPQIHLQVYTALIDFGLDIQQAVEAPRWL
                     AGRFAIGDAREQLNLEGRFPDATVAELARRGHAINRWEAWNELAGHAHGITLDAGAGV
                     LVGGADPRSDGAAIGY"
     gene            5940..7169
                     /locus_tag="VNU03_19045"
     CDS             5940..7169
                     /locus_tag="VNU03_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HWP76626.1"
                     /translation="MQEVSVDRLIALVAFAGTYLGLALGRLPFFRVDRTGVAIIGGAV
                     VVVSGLLPWDRAVAAVDAHTLVLLFGMMIVAAYLRLSGFFRLVTYAAVRRAHTPVGLL
                     ALIIVAAGVLSALFVNDVVCLVMAPIVLDLVRRLRLPPVPYLIALATAANVGSVATLT
                     GNPQNMLVGSFSGISYRAFLLREAPVAVIGLALVFAVIWLAYRRRLPPALPAEALEER
                     GAVHQALMIKTMAAVSVMLVAFLAGVPIALVAIGGAAYCLLTRRVNPDKVYREIDWGL
                     LVLFTGLFVVIGGLEASGLTGEILGWAGAVGLYRPAVLTAVTAVLSNLVSNVPAVLLF
                     KAVIPTFGEPTRAWLLLAMASTLAGNLTILGSVANLIVVEQARGAGIQIGFLEYSKVG
                     VPVTVATLLVGWLLLIL"
     gene            7188..>8330
                     /locus_tag="VNU03_19050"
     CDS             7188..>8330
                     /locus_tag="VNU03_19050"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWP76627.1"
                     /translation="MALLADLTELRARVAASGGRLEKRRLVALYLRALPPDDLARAVT
                     YLSGRALPASDPRVLNVRGLPAPAPGASGEPLTLADVARAFGEVAEAAGTGARRLREA
                     RLGELAARASEAERDLLQRIIYGEMRMGLSEGLVLEAIAEAAGAPPATVRRAALITGD
                     LTAVAALALGGDAAALAAAQPRVFVPLLPMLAEIATDPAEALAAHGGTSAMEYKYDGA
                     RIQLHRDGERVAIWSRRMSDVTRSLPDIVAIARRDLSGTPLILDGEVVALDPAGRPLP
                     FQELMRRFRRVRGVEAAAGALPLTLHLFDCLMADGRSYLDEPYARRWEVLERVTGGRW
                     LAERALVTRAEDGAALMARALAAGHEGLMAKDPASPYEPGGRGKKWL"
BASE COUNT          965 a         3077 c         3091 g         1197 t
ORIGIN      
        1 ggcgctgatc cgcgccgggg ccggggtgga gctggtcggg cccgatctct tcgtcccgct
       61 cggccacggc cgcccgcatc tccgccgcgt gctttccgcg ctcgcgctct acgagccggc
      121 cgcccccggc tccgcgaccg cggccccggt ggacggcgag ccgtccgtcg gcccgcggcg
      181 gctgcgcgag atccgggtgc cgctcgcctg acatgtcggc cctcctcgcc ctccgcctcg
      241 tcacctacct gctggtcggc accggcgtgg ccgcgctcta cggcgccgcg ctcatcggtc
      301 cggtgggcgc ggggctggtg gtgctggcca tcctggtggg ctggggccac gagcaggcgc
      361 gggcccgcgg cgcggtgcgc ccggcgctcg gttactgcct ggtcggcacc gcggcggtgg
      421 cgatcacggt cgatctcttc tacctcgcct actcgctgct ggacgggatg gtgcacctgc
      481 tgctgttcct catcctcctg cgcctcttcg tgcggcgcgg cctgcgggac ctgcgcgacg
      541 ccgcgctgct gtcgttcttc atgctggtcg ccgccgcctc catcagcttc agcttgagct
      601 tcctggtggt gtacgtcgtc ttcctcgtgc tcggcacctg gatgctgatc ctcaaccacg
      661 tggtgaccga gctcgagcag gcgggccgcg agggcgcggc cggctcctcc acccgccgcg
      721 tcttcttccg cgggccgctc acgcgggtga gcctggtcgc cgccgcgatc accttcgcgc
      781 tcgcgggcgg gctcttcttc atcattccgc gcgtggggca ggccgcgctg cccctgcggg
      841 cccagctcgg ccgcatggtg accggcttct cggaccgggt ggacctgggg agcttcggcg
      901 acatcgagag cgatccgagc gtggtgatgc gggtgtacct caccgacgag cgggtggagc
      961 cggccacgct gcccgagctg cgctggcggg gcgtggcctt cgaccagttc gacggcagcg
     1021 cctggtcgag cgcgggggcg acgcgccgct acatccgccg cggcgccgcg ggcgacttcg
     1081 ggatcggcct gccgcgcggc cccggaccgg tcctgcgcca ggagatcttc ctggagccga
     1141 tgggcaccga tgcggtcttc ggggcgcccc ggatgctgcg gctcgagctg cagtcgccca
     1201 ctctcgtggc ggacgacatg ggcgggctca gcacctcgag cgccaccgcc cggctgcact
     1261 acatcgtcga gtccgagctg gaggagccga cccggccggc cggtctcgcg gcgaggtcgg
     1321 gcctgccggc ggcggagcgt gcgcgctacc ttcagctgcc cgagctgtcc cccgacgtcg
     1381 cgcggctggc ccgcgaggcg accgcgggca cccgggaccc cgagaccgcg gcacgccgca
     1441 tcagcgcgtt cctcagcacc acctaccgct acacgctcgc gcttcgccgc gagaccacgc
     1501 gcccgcccct cgaggagttc ctgttcgtgc gccggtcggg caactgcgag tacttcgcgg
     1561 ccgcgatggc ggtgatgctg cggagcgagg gcatcccgag ccgggtggtg gcgggcttcc
     1621 agcacggcga gtggaacccg tacggccgct acttcatggt ccggctgagc gacgcgcact
     1681 cgtgggtcga ggcctacatc gacgggcggg gctgggtcgc cttcgatccg tcgccccgcg
     1741 gcgaggtcgc gagcgtcccg cccggatcgg tctcgctcta cctcgacgcg gcgcgcatgc
     1801 gctggtaccg ctacgtgatc aactggagcc tgggcgacca ggtgcacctc gcggccagcg
     1861 tgcatcgcca ggccaccgac gtgcgggtcg ggctctcgtg gccgcgccac tggcgggtga
     1921 gcccggcggt gctggcggcc ggcggagcgg tggtggtgct ggccctggcc tggctattcg
     1981 ggcgtcgcgg cttcgccaag ggcggggcga aggccaccgc gagcgtgccc ccgttctacc
     2041 agcgcgcgct gcgcctgctc gcccggcgcg ggctctcgcc cggggcggcc gagaccgcgc
     2101 ggcagttcgc gacgcgggtc ggcgtcaccg ctccggagcg cgcggagcct ttcgcgcgcc
     2161 tcacgcgcca ctacgagcgg gcgcgcttcg gcgcgaccgc gctcagcgag acggagtggc
     2221 aggacgtgac gctcgcgctg gacacgctcg ccgccgcgcg ctgagcccgg cccgcggcgg
     2281 gcgagatcgt ccactccacg cggagctcct ccccggcgcg cagcgaccgc cagcgggcga
     2341 ggatctcgtc gagggccgga cccaccggag tggccgtctt ggcgcgccac gcggccgcgt
     2401 gggcgttcac gacgcgctcg gtggcgacga gcgccgcgtg atgcgtgcgg atcacccgcc
     2461 ggcccggctt cgtgggacgt cgccggacgc tctcgaaggt cgcgccgatc gagagcaggc
     2521 cccagaaccc gcaccgcagg ccgagggtgg actcgacgac gaactgggcg aggtcgtgcg
     2581 gcaggtctcg ccccgcggcc atcgtggtcc cttgcaatgg ccggcgcttg ggaatgagcg
     2641 cggtccaggc gcacaggcgt ccgccgtcga ccttgtagaa cgcgacccgc atcgacatgg
     2701 ggcaggaata ctagcgcagg cggcggtgct cgaccagcag gcagcggtcc tggaccacct
     2761 ggatgcccgc gcgcgcgaag gtctccgcgg cctgctcgtg gcggatgccg agctggagcc
     2821 acaccacgcg cgggtgcgcg gccaggatgt cgtccacgtg cggaggaatg tcgcgcgggc
     2881 ggcggaagac gtcgaccatg tccaccgggc ccggcacctc gcggacggtg cggtagacgg
     2941 ggcggtcgag gatgcgggtg gcctcgggga agtagaccgg caccggcacg acgtcgtaac
     3001 cggcgctcgc catgtactcg gccacgtaga atccgggctg gtcgcgctgc gcctcggtct
     3061 tgatgcccag gaccgcgatg cgcttgcact cgcgcacgat gcgcgcgatg gactcctggt
     3121 cggtcaccag gttcgcgcgc cacgcctcgc tcatggccag agtctagcac ctgtgctagc
     3181 ctcggcgcgg gaggaccccg gatgcccgtc cgcatcaccc gcgtctacac gcgcaccgga
     3241 gaccacggcg agaccgcgct cgtcggcggc cgccgcgtgc cgaaggattc gccgcgcatc
     3301 gaggcctacg gcaccatcga cgagctgaac gcgatcatcg ggctggcccg cgtcttcaac
     3361 gcggagcgga agaagagcgg caagcggcac cggtggctcg acgaggtgct gcgccggctg
     3421 cagaacgagc tcttcgacct cggcagcgag ctggccaccc ccgccgacgc gacctacgag
     3481 ggcatgttcc gcgtgagcgc cgagcaggtg accgcgctcg agcggctgat ggacgagtgc
     3541 cagaaggagc tgaccccgct caagagcttc atcctgcccg gcggcgggcc ggtgggcggc
     3601 ttcctccacc aggcgcgcac cgtgtgccgc cgggcggagc ggcgcgtgct ggagctctcg
     3661 cgggtggagg cgatcggcga gtggccgctc aagtacgtga accgcctcag cgacctgctc
     3721 ttcgtgctct cgcggtgggt cggcaagcac ctgggcgaga ccgagtatct ttggcagcgc
     3781 gggctcgacg cgcacgcccg gaagcgctag gtggccgagg actaccagat ccgccgcgcg
     3841 ggccccgccg atcacgcggg ggtcacccgc gagctggccg agtacttcgc gttcctgggt
     3901 gaggacgtgg acgcgagcgg cctcgaccac gacgtggccg actggcaggg cgagtacgac
     3961 ggcgcggcgg gggtgatgct gatcgtcgtc gaccccgccg gcgtggtcgt cgggaccgcg
     4021 ggggtgcgcc gcctcgcccc gggcgtggcc gagctcaagc gcatgtggct gcgcccggcg
     4081 tgccggggcc gcggcctcgc ggaccggctg atggcccgcg tgctggagga ggcccgcgcg
     4141 ctcggcggag caacgctgcg cctggacagc gagagccggc tcgaggccgc ggtgcggctc
     4201 tatcgccgct acgggttccg ggagatcgcc gactacaacg gcaacccgcg cgccgacgtc
     4261 tggatggagc tgccgctcgc gccgggacgg agctaggcgc atcctcgaca ccagccgccc
     4321 ggccacgctg gcgtaccgcg gcatggtcac ctcgccccac tcgctggcct cgcgggccgg
     4381 ggtccaagtc ttacaatccg gcggctccgt ggtcgacgcg gccatcgcgg ccgccgcggt
     4441 gctcgcggtc gtctaccccc acatgaccag cgtggggggc gacgcgttct ggctcatcca
     4501 cgaggcgcgc accggctccg tgcactcgct cgacgccgcg ggcggcgcgg cggcgtcggg
     4561 gaccctcgac tggttccggg cccgcggcct cggccagatc ccgctccgcg gtgtcgtgcc
     4621 cgcgacgctc acggtgcccg gcgcggtgga cggctggtgc gaggcccacg cgcgccacgg
     4681 gaagctcccg ctcgccgacg atctggccgc ggcggtgcgc tacgcgcgcg acgggttccc
     4741 gatcacccgg cggatctccg agtggatcga gcgcagcgcc gacgtgctca cggccgatcc
     4801 gcccgcggcg cgcatcttcc tggccgaggg gcgccggccg gcggtcgggc ggcggctcgt
     4861 caacccggac ctcgcgcgca ctctcgagcg catcggcatc gggcgggccg gcttctacga
     4921 gggcgagacc gcccgcgaga tggcccggtg ggcgcgcgag cacggaggct tcttcaccga
     4981 gcacgatctg gccgggcagc gcgcgcgctg gggcgagtcg atccgcgcca cctatcgcgg
     5041 cgtcaccatc cacgagaccc cgccgccgag ccagggcatc tcggtcctgc aggcgctgcg
     5101 gctcgtggaa tcccacgacc tcggcgcgct gccctacctg gggccggacc acgtgcacct
     5161 gctggtgcag gccaagcaga tcgcattcca cgatcgggac cgcttcgtcg ccgatccggc
     5221 cttcgtgccg gtgccggtcg agcgcctgct ctcgaccgcc tacgccgacg agcgccggcg
     5281 cctcatcgat cccgcccggg cgctgccgtg ggaccgcgtg ccctccgaag ggagcctggc
     5341 cggcgacacc gtctacgtgg ccgcggtgga tgaggcgggc aacgcggtct cgctgatcca
     5401 gagcctctac ttcggcttcg gcgcgggggt ggtggcgggg gacaccggga tcgtgctcca
     5461 gaaccggagc gcgtacttct cgctcgatcc ggatcacccg aatcggctcg agcccggcaa
     5521 gcggccgctg cacaccctga tcgcctcgct cgccttccgc gacggccgcc tgtggcaggt
     5581 ggtgggctgc atgggcgccg acggccagcc gcagatccat ctccaggtgt acaccgcgct
     5641 gatcgacttc gggctcgaca tccagcaggc ggtcgaggcg ccgcgctggc tcgccgggcg
     5701 cttcgcgatc ggcgacgcgc gggagcagct caacctggag ggccgcttcc cggatgccac
     5761 cgtcgcggag ctggcccgcc ggggccacgc gatcaaccgg tgggaagcct ggaacgagct
     5821 cgccggccac gcccacggca tcaccctcga cgcgggcgcc ggcgtcctgg ttggcggcgc
     5881 cgatccgcgg agcgacggcg ccgcgatcgg ctactgaacg gccgcccggg gatcggccca
     5941 tgcaggaagt cagcgtcgac cggctgatcg cgctcgtcgc gttcgcggga acgtatctcg
     6001 gcctcgccct cggccgcctg cccttcttcc gcgtcgaccg caccggcgtg gccatcatcg
     6061 gcggagcggt cgtggtggtg agcgggctcc tgccctggga ccgggcggtg gccgcggtcg
     6121 acgcccacac cctcgtgctg ctcttcggca tgatgatcgt ggccgcgtac ctgcgcctct
     6181 ccggcttctt ccgcctcgtg acctacgcgg cggtgcggcg cgcccacacc ccggtcggcc
     6241 tgctggccct catcatcgtg gccgccggcg tgctgtcggc gctcttcgtc aacgacgtgg
     6301 tgtgcctggt gatggcgccc atcgtgctgg atctcgtgcg ccggcttcgc ctgccgcccg
     6361 tgccgtacct gatcgcgctc gccaccgcgg ccaacgtggg cagcgtggcc accctgaccg
     6421 gcaacccgca gaacatgctg gtgggcagct tctccggcat cagctaccgc gcgttcctgc
     6481 tgcgcgaggc gccggtggcg gtgatcgggc tcgcgctcgt gttcgcagtg atctggctcg
     6541 cgtaccggcg ccggctgcca ccggcgctgc cagccgaggc cctcgaggag cgcggcgcgg
     6601 tgcaccaggc gctgatgatc aagaccatgg ccgcggtgtc ggtgatgctg gtggccttcc
     6661 tcgccggggt gccgatcgcg ctggtggcca tcggcggcgc cgcctactgt ctcctcacgc
     6721 gacgggtgaa tcccgacaag gtctatcgcg agatcgactg ggggctgctc gtgctcttca
     6781 ccgggctctt cgtggtcatc ggcggcctcg aggcctccgg gctgaccggg gagatcctcg
     6841 gctgggcggg cgcggtcggc ctctaccgcc cggcggtgct cacggcggtg accgcggtgc
     6901 tgtcgaacct ggtctcgaac gtccccgcgg tgctgctctt caaggcggtg atcccgacct
     6961 tcggcgagcc cacccgcgcg tggctcctgc tcgcgatggc ctccacgctg gccgggaacc
     7021 tcacgatcct gggctcggtc gccaacctca tcgtggtcga gcaggcgcgg ggcgccggca
     7081 tccagatcgg cttcctcgag tacagcaagg tcggggtgcc ggtgaccgtg gcgaccctgc
     7141 tggtcggctg gctcctgctg atcctgtgac ggagagagaa tggcgccatg gcgctcctcg
     7201 ccgatctgac cgagcttcgc gcgcgcgtcg ccgcgagcgg ggggcggctc gagaagcgcc
     7261 gtctcgtcgc cttgtacctg cgcgcgcttc cgcccgacga tctcgcacgc gcggtcacct
     7321 acctgagcgg gcgggccctc ccggcctcgg atccgcgcgt gctcaacgtg cgcggcctgc
     7381 ccgcgccggc gccgggcgcg tcgggcgagc cgctcacgct ggcggacgtg gcgcgggcgt
     7441 tcggcgaggt cgcggaggcg gccggaacgg gcgcccggcg gctgcgggag gcgcgcctcg
     7501 gcgagctggc ggcgcgcgcc tcggaggccg agcgcgacct gctccagcgc atcatctacg
     7561 gcgagatgcg catgggcctc tcggaggggc tcgtgctcga ggcgatcgcc gaggcggccg
     7621 gcgcgccgcc cgcgacggtg cgccgcgccg cgctgatcac cggcgatctg accgcggtgg
     7681 cggccctcgc gctgggcggg gacgccgccg ccctggccgc ggcccaaccc cgcgtcttcg
     7741 tgccgctgct gccgatgctg gccgagatcg cgaccgaccc ggccgaggcg ctggcggccc
     7801 acggcggcac ctccgcgatg gagtacaagt acgacggcgc gcgcatccag ctgcaccgcg
     7861 acggcgagcg ggtggccatc tggagccggc gcatgtccga cgtcacccgc agcctgcccg
     7921 acatcgtggc catcgcccgg cgcgatctct ccggcacgcc gctcatcctg gacggggagg
     7981 tggtcgccct cgacccggcc gggcgcccgc tgccgttcca ggagctgatg cgccgcttcc
     8041 gccgggtgcg cggcgtcgag gccgccgcgg gcgcgctgcc gctcacgctg catctcttcg
     8101 actgcctgat ggcggacggg cggtcgtatc tcgacgagcc ttacgcgcgc cgctgggaag
     8161 tcctcgagcg cgtgaccggc ggtcgctggc tggccgagcg cgcgctggtc acgcgggcgg
     8221 aggacggcgc cgcgctcatg gcccgcgccc tcgcggccgg ccacgagggc ctgatggcca
     8281 aggacccggc gagcccctac gagccgggcg gccgcggcaa gaagtggctc
//
LOCUS       DATMCV010000828         5813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_381058, whole genome shotgun sequence.
ACCESSION   DATMCV010000828 DATMCV010000000
VERSION     DATMCV010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381058"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /gene="tssK"
                     /locus_tag="VNU03_19055"
     CDS             complement(<1..283)
                     /gene="tssK"
                     /locus_tag="VNU03_19055"
                     /inference="COORDINATES: protein motif:HMM:NF017723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HWP76628.1"
                     /translation="MTPLGKVVWGEGMFLTPHLFQQADRYHEQVLDFRVRAVVPFGWG
                     FTELDLDDEAVTNGDVGLRRCAGVLPDGLVFQVPQPDRGPSTRSIKSHFT"
     gene            complement(306..3713)
                     /locus_tag="VNU03_19060"
     CDS             complement(306..3713)
                     /locus_tag="VNU03_19060"
                     /inference="COORDINATES: protein motif:HMM:NF025689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HWP76629.1"
                     /translation="MIYLKHVEGSKRGQVEEFDLERIRVGRHPDNDLRFDPSVDREVS
                     GHHAEILCQGEQVSIRDLQSRNGTLVNGRRINQTTALRHGDTIQFAPTGPKIIFSLGE
                     AVHGTGTLVIDRDQIAPAAPAAAERAAPAAATPPAAPQRKRTFVLAGAVVVLVLVVLL
                     LALAAWRSWKLFLVLLGVVIFLGVAGAVAWWWMRTWSSSEPAASPGEPVRPPGGETGP
                     ATGEGASDTIRELRAKWSQALARLRGSKLERQGDGAVRALPWIVTLGERGSGKSELIR
                     AGNPPASVSTVRQAVSGTRTCDWWFFDRVVVLDTPGRYTFPVDAKGDGREWEEFLGLL
                     RSARQADPLNGVIVTVAADSLAARSTASLKDEATQIRRRIDSLTQRVGVRPPIYLVVT
                     KIDLLAGFKEFFADVPEANRSQAMGWVSEDDREEVSAALFGRAVETIARQLDRLRLSV
                     LDRRDADPQVGRRFLFPDEFRALTGPLQVFGDALLRANQYDETPWFRGVFFASATGDG
                     APTSYLARTLGLDDGLSSGAPRSSGSLFVRDLFGAILPRDRELVRRTAAARRRHSRGR
                     QGAAYAVGAGAAVLCLLLTVSFVRNSRSLARLDVRPCLGSGGTAVTASLGARLERLDR
                     CRGTLATLVPHGFWDRVTTDFWLRQAERLADPIARRYVEVFTAEVEGPIEGALDRALV
                     PGPKAPVVVGAVLQRIAVIGRCRSEGRCPTDDAGAWPSYRTLVGAVEPRAQDNEDTVK
                     ALRRTHAAYLDLQAASALDDMRGRDVARVSRWLSAGGLRSEWILASASSQFAPVRSRD
                     FWGWDGPLQVDAPFTGRAWSEAIQPLTQGLRALAPNAADVRDAVTRFESDYRAEALRQ
                     WGQFLASFPQEERVLGGRRGSRDLAVRSLEADSPYRRVLDQAAADVGAIAGPVHGEGE
                     LPAWAGTLLRYAALRRNAAGPPSAAGQKLGDAKAQYTEAERQAMGYVKAYGEAMDQLK
                     GELGGPEKAYASVQKVFEEGESSERSVHPVHRVLWNLRALRNMIGARQGDDHIVWDLL
                     SRPVGLVWRTMLAEAAQQVQGVWEAMIPGLSGLTPGLQAAKIIEFANGPAGPLLERSR
                     DRYVVRRFLGEAAPLSPGFVDFISRIRWMPVERLDKIDPPRLIVTSS"
     gene            3974..5002
                     /locus_tag="VNU03_19065"
     CDS             3974..5002
                     /locus_tag="VNU03_19065"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HWP76630.1"
                     /translation="MPVRRVTCRYERLLLVGVTAGLIALALVPDGLTRKSFALAAAGW
                     QGGQYRDSSNQAAGPDPAATTRAPAKQLALGETAVQRPRLQPGEAATLRASYEVPEPI
                     QVRETRVIRYDGLVLARIERVIARPGGSVTSEYRVDVPRDAAEGWYAVTTTVERVDAG
                     TRGGAGEQKETGFFVDTKATATSAGSTSAPPAPKAAEDGIAVKLWSEKPRYRVGDRLT
                     ISFETNRDAFLTIVNVGTSGEVTILFPNRFSGGHGVKAGRTYRIPEATDSYELELKGP
                     PGVELVYALLTLKPVLFLATDFPSAGAVFPSTAERVSRVTRDINVAAKSIPLREQAQA
                     MIELEISR"
     gene            5005..>5813
                     /locus_tag="VNU03_19070"
     CDS             5005..>5813
                     /locus_tag="VNU03_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HWP76631.1"
                     /translation="MTPRRSWSPVSPIARRVVLGVLSLVVLGCAPAPAPKAAEPRPTP
                     SGQAADAFLVVDCLLPGQIRRLGNQVTYVGARRAEKATARDCEIRGGEYVAYDRADYK
                     TALQVWMDGAQRGDPKSQMYVGEIYEKGLGVPPDYAAAAQWYRRAAEQGYAPAALSLG
                     VLYENGLGVPRDPKQAAQWYGRATGASTVTVNASGAGREDVRRLEAQVESLRADLRTK
                     QAELDQNQRGLAEARRALAQVQGDSQTTRTELDRLRRERASVQGTDAATVTR"
BASE COUNT          918 a         2082 c         1993 g          820 t
ORIGIN      
        1 gcgtgaagtg cgacttgatc gagcgggtgc tcgggccgcg gtcgggttgc ggcacctgga
       61 agacgagccc gtccggcagc acgcccgcgc agcgccggag gcccacgtcc ccgttggtca
      121 ccgcctcgtc gtcgaggtcc agctcggtga aaccccagcc gaatgggacc acggcccgca
      181 cgcgaaaatc gaggacctgc tcgtgatacc gatcggcctg ctggaagaga tggggcgtga
      241 ggaacatgcc ctcaccccat acgaccttgc ccaatggcgt catggcgtgg ctcgctcgaa
      301 gcctttcacg aggacgtgac gatgagtcgt ggcggatcga tcttgtccag ccgctccacc
      361 ggcatccagc ggatgcgcga gatgaaatcg acgaaccccg gcgacagggg agccgcttcc
      421 ccgaggaagc ggcgcaccac gtaccgatct ctcgaccgct cgagcaaggg cccggccggt
      481 ccgttggcga actcgatgat cttcgccgcc tgtagcccgg gtgtcaggcc gctcaggcct
      541 ggaatcatgg cctcccatac gccctgcacc tgctgggcgg cctcggcgag catcgtccgc
      601 cagaccagcc cgaccggtcg cgatagcaag tcccacacga tgtggtcgtc tccctggcgt
      661 gccccaatca tgttgcgcag ggctcggaga ttccagagca cccgatggac cggatgaacg
      721 ctccgctcgc tggattcccc ttcctcgaac accttctgaa ccgacgcata ggccttctcg
      781 ggtccgccga gctcgccctt gagctggtcc atggcctccc cgtatgcctt cacgtacccc
      841 atggcctgcc gttcggcctc ggtatactgc gccttcgcat cgcccagctt ctgcccggcg
      901 gcactcgggg gacccgcggc attccgtcgg agcgccgcgt agcgaaggag cgtgccggcc
      961 cacgcgggca gctcgccctc cccgtggacc gggccagcga tcgcgccgac atcggcggcg
     1021 gcctggtcga gcacccggcg atacggcgaa tccgcttcca gggatcgcac cgcgaggtcc
     1081 cgcgacccgc gccggccccc gagcacgcgc tcctcctgcg ggaagctcgc caggaactgc
     1141 ccccactggc gcagggcctc ggctcgatag tcgctctcga atcgcgtgac ggcgtcacgc
     1201 acgtcggccg cattcggcgc gagcgctcga aggccttgcg tcagcggctg gatggcctcg
     1261 ctccacgccc ggccggtgaa gggcgcgtcg acctggagcg gaccatccca gccccagaag
     1321 tcccgggagc gcaccggcgc gaactgactg ctcgcggagg ccaggatcca ctcgctccgc
     1381 aagccgcccg cgctcagcca ccggctgacg cgcgccacat cgcggccacg catgtcgtcg
     1441 agcgcggagg ctgcctgcag gtccaggtac gccgcgtgag tccgccggag ggccttgacc
     1501 gtgtcctcgt tgtcctgggc tctcggctcc acggctccga cgagcgtgcg atagctgggc
     1561 caggctccgg catcgtccgt cgggcagcgg ccctcggacc ggcagcggcc gatcaccgcg
     1621 atgcgctgga gcaccgcgcc gacgaccacg ggggccttcg ggcccggcac gagcgcgcga
     1681 tccagcgccc cctcgatcgg gccctcgacc tccgccgtga agacctccac gtaccggcgg
     1741 gcgatggggt ccgccagtcg ctcggcctga cgcagccaga aatcggtcgt gacccggtcc
     1801 cagaagccgt ggggcacgag ggtcgcgagc gtgccgcgac accggtcgag gcgctcgagg
     1861 cgagcgccga gcgaggcggt cacggcggtc ccccccgatc cgaggcaggg gcgcacgtcc
     1921 agccgcgcca gggaccgtga attccggacg aaggagacgg tcagcaggag gcacaggacg
     1981 gccgcacccg ccccgaccgc gtacgcggcg ccctggcggc cgcggctgtg gcgacggcgg
     2041 gccgcggcgg tgcgccgcac cagctcgcgg tccctgggca ggatggcgcc gaacaggtcc
     2101 cgcacgaaga gcgagccgga cgaccgcggc gcccccgacg acaggccgtc gtccaggccg
     2161 agggttctcg ccaggtagga cgtgggcgcc ccgtctccgg tcgcggatgc gaaaaagacg
     2221 ccccgaaacc agggggtctc gtcgtactgg ttggcgcgga gcagggcgtc gccgaacacc
     2281 tgaagaggcc ccgttagggc ccggaactcg tcggggaaca ggaaccgccg gccaacctgc
     2341 gggtcggcgt cacggcggtc caggacggag aggcgcaggc gatcgagctg tcgcgcgatc
     2401 gtctcgaccg cgcggccaaa cagcgccgcg gacacctcct cacggtcatc ctcgctgacc
     2461 cagcccatcg cctggctgcg gttcgcttcc ggcacgtcgg cgaagaactc cttgaaccct
     2521 gccaggaggt cgatcttggt gacgaccagg taaatcggag gccgcacccc gactcgctgg
     2581 gtcaggctgt cgattcgccg gcgaatctgg gtggcttcgt ccttgaggct ggccgtcgag
     2641 cgtgcggcga gcgagtcggc cgccaccgtg acgatcacgc cattgagcgg gtccgcctgt
     2701 cgcgcgctcc ggagcagccc gagaaattcc tcccattcac gtccgtcgcc cttcgcgtcg
     2761 accgggaagg tatatcgtcc cggcgtatcc aggaccacga cgcgatcgaa gaaccaccag
     2821 tcgcacgtgc gggtccccga caccgcctgt ctgacggtcg acaccgacgc cgggggattg
     2881 ccggccctga tgagctcgct cttccccgag ccccgctcgc cgagcgtcac gatccagggc
     2941 agcgcccgca ccgcaccgtc cccttgacgc tcgagcttgg agcctcgcaa tctggcgagg
     3001 gcctggctcc acttggcccg cagctcccgg atggtgtccg aggccccttc gccggtggcc
     3061 gggccggtct cgcctccggg aggccgaacc ggctcccccg gcgacgcggc cggctcgctg
     3121 gacgaccacg ttcgcatcca ccaccaggcg accgctcccg ccacgccaag gaagatgacc
     3181 acgcccagga ggaccaggaa cagcttccac gagcgccatg ccgcgagggc gagtaggagg
     3241 acgaccagca cgagcacgac caccgccccg gccagcacga acgttctctt ccgctgcggc
     3301 gccgccgggg gtgtggccgc cgcgggggcg gcccgctcgg cagcggcggg ggcggccggg
     3361 gcaatctggt cgcgatcgat cacgagcgtc ccggtgccgt ggacggcttc gcccagcgag
     3421 aagatgatct tgggaccggt gggcgcgaac tgaatggtgt cgccatgccg cagggccgtg
     3481 gtctggttga tccggcgccc gttgaccagg gttccgttgc gactctggag atcacggatc
     3541 gagacctgct cgccctggca caggatctcg gcatggtggc ccgagacctc ccggtccacg
     3601 ctgggatcga acctgaggtc gttgtccggg tggcgcccga cgcgaatgcg ctcgaggtcg
     3661 aattcctcga cctgcccgcg cttgctcccc tcgacgtgct tcagatagat catgcgctct
     3721 caggaactgg gatggacttc gcgaaggaag ggacggtgca aagccacggc tcgacggcag
     3781 tatgacgacc tagtcgcaac aagatctcga tctcaagcgc cccctgcccc ccgggtgagc
     3841 ctagatggcc tggtagaccg cagagtagta gagcacctga cggggagtca agcttgactc
     3901 cccgcaccag ggcgacgtag tgttaccgcg ttgggaaacc gatttgcggg agccctctcc
     3961 cgagaggaag acgatgcccg tccgccgtgt cacctgccgg tacgaacgcc tccttctcgt
     4021 gggtgtgacc gcgggcctga ttgccctggc cctcgtaccg gacggcttga cgcgcaaaag
     4081 tttcgccctt gccgcggcgg gctggcaagg agggcagtac cgggactcca gcaaccaggc
     4141 ggctggtccg gatcccgccg caaccacccg agcgcccgcc aaacagctgg ccctcggcga
     4201 gaccgcggtg cagcgcccgc gcctgcaacc gggtgaagcg gccacgctcc gggctagcta
     4261 cgaggtaccg gagcccatcc aggtccggga aacccgggtc atccgctacg atggcctcgt
     4321 cttggcccgg atcgagcgcg tgatcgcgcg ccccggaggc agcgtcacga gcgagtatcg
     4381 ggtggacgtg ccccgggacg cggccgaggg ttggtacgcg gtgacgacga cggtcgagcg
     4441 ggtagacgcg ggaacccggg gcggcgctgg cgagcagaag gagacgggat tcttcgtcga
     4501 cacgaaggcg acggcgacca gcgccgggtc cacgtcggca ccgccggctc ccaaggccgc
     4561 cgaggacggg atcgcggtga agctgtggtc ggagaagccg cgctaccgcg tcggcgatcg
     4621 gttgaccatc agcttcgaga ccaatcgaga cgcgttcctc accatcgtca acgtgggcac
     4681 gagcggagag gtgacgattc tctttccgaa ccgcttctcc gggggacacg gcgtcaaggc
     4741 aggacggacg taccgcatcc ccgaggcgac cgacagctac gagctcgagc tcaagggccc
     4801 tcccggcgtc gagctcgtct acgccttgct taccctcaag cccgtgctgt ttctcgcgac
     4861 cgatttcccg tccgccggcg ccgtcttccc ctcgactgcc gagcgcgtgt ctcgcgtcac
     4921 ccgcgacatc aacgtggccg cgaaatcgat cccgctccgc gagcaggccc aggccatgat
     4981 cgagctggag atcagtcgct agccgtgacg ccccggagat cctggagtcc cgtctcgccc
     5041 atcgcccgtc gcgtcgtcct cggcgtcctc tcgcttgttg tactgggctg cgctccggct
     5101 ccggccccga aagcggcgga gccccgaccg acgccgtcgg gacaggcggc ggacgcgttc
     5161 ctggtcgtgg actgcctctt gcccgggcag attcgccggc tcggcaacca ggtgacctac
     5221 gtgggagcgc ggcgggccga gaaggccacc gcccgcgatt gcgagatccg aggcggtgaa
     5281 tacgtcgcct acgaccgcgc cgattacaag accgcgttgc aggtctggat ggacggcgcc
     5341 cagcgcgggg atcccaagtc gcagatgtac gtgggcgaga tctacgagaa gggtctcggc
     5401 gtgcctccgg actacgccgc ggcggcgcaa tggtatcgcc gcgcggccga gcagggttat
     5461 gccccggccg ccctcagcct cggggtcctg tacgagaacg ggctcggcgt gccgcgagac
     5521 cctaagcagg cggcgcagtg gtatggccgg gcgaccggcg ccagcaccgt caccgtcaat
     5581 gcctcgggag ccggccggga ggacgtccgg cgactcgagg cacaggtcga gagcctccgc
     5641 gccgacctgc gcaccaagca ggctgagctg gatcagaatc agcgtggtct ggccgaggcg
     5701 cgccgggcgc tcgcccaggt ccagggcgac tcccagacca cgcgcaccga gctggaccgc
     5761 cttcgccgtg agcgggcctc cgtccagggc accgacgcgg cgacggtgac gcg
//
LOCUS       DATMCV010000829         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_979826, whole genome shotgun sequence.
ACCESSION   DATMCV010000829 DATMCV010000000
VERSION     DATMCV010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979826"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VNU03_19075"
     CDS             complement(<1..267)
                     /locus_tag="VNU03_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76632.1"
                     /translation="MRTLLPVGLMAAAHLLVTLGLGVLTFLTQGPLDRPGPPSLVHRT
                     LSATVDVLEFPAVWALRRVDRERLRGFEAVAVANSLLWGAGLVFS"
     gene            complement(270..1412)
                     /locus_tag="VNU03_19080"
     CDS             complement(270..1412)
                     /locus_tag="VNU03_19080"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP76633.1"
                     /translation="MRAERLLEAVRALAPLVDSERARLDRDRALSPGLVDALVDAGLF
                     RLWTPRRLGGAELDPVAGLRVIAAVAALDGSVGWNVMVASAHGFLAGRLPAVAAREVF
                     GDRRAVVAGQLEPGGVADVVKGGYRVSGRWPFASGCRHATWLLAHCVVPTGSRRRAGA
                     RPTTRLVFVPVDRARIHDTWRVSGLRGTGSHDYSLEGVMVPAAYAIDAFADEPTRPER
                     LYAYPVIPLVTGAVGAVPIGIARGAIEELTRLARTRKRAGRPLAEDATVQQGIARAEI
                     HARTAESLLHEAVGDMWRTVKRGKPPTLEQRAHVRMACVNAGESAAAAVDLVWGLAGS
                     RAIFEDAGLERRFRDVHVATQHVALSPRTLEMAGRVLLGLEPLGLF"
     gene            complement(1437..1802)
                     /locus_tag="VNU03_19085"
     CDS             complement(1437..1802)
                     /locus_tag="VNU03_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HWP76634.1"
                     /translation="MIQVANVLVALVALIHLYFLVLEMFLWDTPKGRRTFGLTPEFSA
                     ASKTLAANQGLYNGFLAAGLVWGLTLGAGGLGVKVFFLACVVVAGVFGALTAGRKILW
                     VQALPGAVALALVLLSRGQ"
     gene            complement(1799..2455)
                     /locus_tag="VNU03_19090"
     CDS             complement(1799..2455)
                     /locus_tag="VNU03_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HWP76635.1"
                     /translation="MLDRVAAAVLALPTERTVRVGIDGVDGAGKTTFADELRDRLASS
                     GRPLIRAGVDWFHHPKPVRYRRGRYSPEGHYRDSYDYAALRRLLLDPLGPGGTGRFRR
                     AIFDVDADHAVDAPEERAAPVSILLFDGLFLHRPELRATWDLSIFLRVEWIRNHRLRG
                     APPLDLEAPSTRRYREGQEIYFRECAPWDCASIVVDNDDLDAPFVVEGPARPMTRCSP
                     "
BASE COUNT          382 a          961 c          925 g          267 t
ORIGIN      
        1 cgagaacacc aggccggctc cccacagcag gctgttggcc accgcgaccg cctcgaagcc
       61 gcgcagccgc tcgcgatcga cccggcggag agcccacacc gccgggaact cgagcacgtc
      121 gacggtggcg gagagcgtcc gatgcacgag cgacggcggc cccggacggt cgagcgggcc
      181 ctgggtcagg aacgtgagga ccccgaggcc gagcgtcacc agcagatgcg cggccgccat
      241 cagcccgacc gggagcagcg tgcgcacgct cagaagagcc ccagcggctc gaggcccagg
      301 agcacgcggc ccgccatctc gagcgtccgc ggcgagaggg ccacgtgctg ggtggccacg
      361 tgcacgtcgc ggaagcgccg ctcgagcccg gcgtcctcga agatggcgcg cgagccggcg
      421 agcccccaga cgagatccac cgcggccgcc gcgctctcgc ccgcgttcac gcaggccatc
      481 cgcacgtgtg cgcgctgctc gagcgtcggc ggcttcccgc gcttcaccgt ccgccacatg
      541 tcgccgaccg cctcgtgcag gaggctctcc gccgtgcgcg cgtggatctc ggcgcgggcg
      601 atcccctgct gcaccgtcgc gtcctcggcg agcggccgcc ccgcccgctt gcgggtgcgg
      661 gccagccggg tcagctcctc gatggcgccg cgcgcgatgc cgatcggcac cgcgcccacc
      721 gcgccggtca cgagcgggat caccgggtag gcgtagagcc gctcggggcg cgtcggctcg
      781 tcggcgaagg cgtcgatggc gtacgccgcg ggcaccatca cgccctccag cgagtagtcg
      841 tggctgccgg tgccgcgcag accgctcacc cgccaggtgt cgtggatgcg cgcccgatcc
      901 accggcacga agaccagccg ggtcgtcggg cgagcgcccg cgcgccggcg actgcccgtg
      961 ggcacgacgc agtgggcgag gagccaggtg gcgtggcggc agccgctcgc gaacggccag
     1021 cggccggaca cgcggtagcc gcccttcacg acatcggcga cgccgcccgg ctcgagctgg
     1081 ccggccacca ccgcgcgccg gtcgccgaac acctcgcgcg cggccaccgc gggaagacgt
     1141 ccggcgagga atccgtgcgc ggaggcgacc atgacgttcc agcccaccga gccgtcgagc
     1201 gcggccactg ccgcgatcac ccggaggccc gcgaccgggt ccagctcggc tccgcccagg
     1261 cggcgcggcg tccacaggcg gaagagtccc gcgtcgacga gggcgtccac cagccccggc
     1321 gagagcgcgc ggtcgcggtc gaggcgcgcg cgctcggaat cgacgagcgg ggcgagcgcg
     1381 cgtaccgcct cgagcaggcg ctcagcgcgc atgcgcgtcg cgatccgccg cgcgcctcac
     1441 tggccgcggg agaggaggac cagcgcgagc gccaccgcgc cgggcagggc ctgcacccac
     1501 aggatcttgc ggcccgcggt cagcgcgccg aagacgcccg ccaccacgac acaggcgagg
     1561 aagaagacct tgaccccgag gccgcccgcg ccgagggtga gcccccagac gagcccggcg
     1621 gccaggaacc cgttgtagag accctggttg gccgcgagcg tcttcgaggc cgccgagaac
     1681 tccggggtga ggccgaaggt gcggcggccc ttgggcgtgt cccagagaaa catctcgagc
     1741 accaggaagt agagatggat gagcgcgacc agcgccacca ggacgttcgc cacctggatc
     1801 atgggctgca cctcgtcatc ggtcgagcgg ggccctccac gacgaacggc gcgtcgagat
     1861 catcgttgtc gacgacgata ctggcacagt cccacggcgc gcactcgcga aagtagatct
     1921 cctggccttc ccggtagcgc cgcgtcgagg gcgcctcgag gtccagcggc ggcgcgcccc
     1981 gcagccggtg gttccggatc cactcgacgc gcaggaagat cgagagatcc cacgtcgccc
     2041 gcagctcggg ccggtgcagg aagagcccgt cgaagagcag gatcgacacg ggcgccgcgc
     2101 gctcctccgg cgcgtccacc gcgtgatccg cgtccacgtc gaagatcgcg cgccggaacc
     2161 ggccggtccc gcccggcccg agcggatcga gcaggagccg ccgcagcgcc gcgtagtcgt
     2221 aggagtcgcg gtagtgccct tcgggtgagt agcgcccgcg gcggtagcgg accggcttgg
     2281 gatggtggaa ccagtcgacg ccggcgcgga tcagcggccg cccgctcgat gccagccggt
     2341 cgcgcagttc gtcggcgaag gtggtcttcc cggcgccgtc gacgccgtcg atgccgaccc
     2401 gcaccgtccg ctcggtcgga agcgccagca ccgccgcggc cacccgatca agcatcgccc
     2461 gacgggcggg cgtcatctaa ccgggccgtc cgaagagcgc gatcgcggct cccgacggca
     2521 ctccgcgcac gaccg
//
LOCUS       DATMCV010000830         4497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_840320, whole genome shotgun sequence.
ACCESSION   DATMCV010000830 DATMCV010000000
VERSION     DATMCV010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840320"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VNU03_19095"
     CDS             <1..1255
                     /locus_tag="VNU03_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76636.1"
                     /translation="GSAQRAFTACQNAADDSGITARCEVVARGGAIVARSYAEAQARD
                     DAAGLVRDAMRCGQRPLGRWFWVERAFCDMPWHGAASAKGVVIWNHGIHGTVAQHTAS
                     VPPVFRLLQARGWDVVKIARNNLGETTGEQSLNRAVRRTQEEIAARRREGYGKVVLAG
                     QSFGGYITLDTAESSRDVYGVVAMAPGIRFGGTGNLDTSVTERTLGALTVDRLALVFP
                     RNDTLFGSQVRGPGAARVLARRGGSFLLLDETLDIVDHGGGTTAKFAVRYGPCLVRYL
                     TSPEVGKGVVGCPESLAEQRGAAEELLPRLPGTVKLAPASDRAAEEGGALLGKAWYGI
                     LEPAAEVVSFAIVDVDGVGRRAMFGVASGGRRGGLYPLATGEPGVTFRVADRGTVTVK
                     GTTLTWTATGGRASHAAKLHPLAEP"
     gene            1328..2206
                     /locus_tag="VNU03_19100"
     CDS             1328..2206
                     /locus_tag="VNU03_19100"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76637.1"
                     /translation="MSGPALVHVVVSGLATGCIYALVALSLVIVYNATRTVNFAQGEM
                     LMVSAFVAWSAQHTAGLPLLAAVACGVAAGGLLAFLVERAIIRRAVSATHWDVLIITL
                     GLSLALRAAAGIVWTNEELPFPALFSNRPVAIGPVRVAPVSLGVIGASLVLMLALVAL
                     FRATRFGRAMRAVAQNQTAARLMGISVERVYSGAWVLAAVVGGVAGVLIAPVTFLSTK
                     MGLIAINGFTAAVLGGFGSLPGAVAGGMLLGVIENLAPLYLPTGIRYSVPFLVMIAIL
                     VARPRGLFGSLERRKV"
     gene            2206..3117
                     /locus_tag="VNU03_19105"
     CDS             2206..3117
                     /locus_tag="VNU03_19105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76638.1"
                     /translation="MRAAAWVLGGMAALGWPLVAPRYFVFLATLVAVNAVVAIGLNLL
                     SGYTNQLSFGHAGFLAIGAYTAAILTLRAPALPVPLTLLAAGLVTALVGLALGIPCLR
                     LDGLYLAMATLAFGFVVTEAITNLDWLTRGNDGLRVPLAHLGPWALDTDRSRYYLALA
                     VAAAMILAALNVAATRTGRAFLAIRASEIAAQASGINAAAYKTVAFVLSAFYTGVAGG
                     LFAFVVGFLSPDAFDVFLSVDFLVMIILGGLGSVWGGVAGAAIVTVLNDSLAGFQAYR
                     PLIFGAIMIACMLFAPGGLAAMTRRQS"
     gene            3114..4301
                     /locus_tag="VNU03_19110"
     CDS             3114..4301
                     /locus_tag="VNU03_19110"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76639.1"
                     /translation="MRQRYAVVVLVAAMVVAMLGGLAGRPAVAAEPGVTDTEILLGGS
                     NSFSGPLAFTGTQLTKVGVDLYFRVLNDAGGVHGRKIRTVYYDDGYRPQDAVANTKKL
                     VEQDGIFAVIAPQGSPPVVATLDYLEQGKVPMLFPFQSSTATRNKKYVFSGLVLSDRQ
                     SRMMIDYLAGARRFKRFAALYQDDEYGKSFLTAFEADLGRHSLKLTATEPVKRGVTDV
                     SAQIAKLQAAKPEVTFLVLTPGPAAQALKERQKIGWGDTLMVSTGPLTDETYLGLAGD
                     ASDGVEGLATLPDPLRADNPGVKLYRDHLQKYFPKNEPNRYSLSGYVAGMLFAEGAKR
                     AGRNLTRDGLVTALESLKGFDTGILPPVTIGADHDTQKQGFWVRVENGRFKPLSDWPK
                     SDP"
     gene            4298..>4497
                     /locus_tag="VNU03_19115"
     CDS             4298..>4497
                     /locus_tag="VNU03_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP76640.1"
                     /translation="MTRPPAPLLDIEELSIRFGGLAALSEVDLAVGAGEIFALIGPNG
                     AGKTTVFNIVTGLYRPLAGHVR"
BASE COUNT          551 a         1603 c         1642 g          701 t
ORIGIN      
        1 cggatcggcc cagcgcgcgt tcaccgcgtg ccagaacgcg gccgacgaca gcggcatcac
       61 ggcgcggtgc gaggtggtcg cgcgcggcgg cgcgatcgtg gcccgctcct acgccgaggc
      121 gcaggcgcgg gacgacgcgg ccggcctggt ccgggacgcg atgcgttgcg gccagcggcc
      181 gctcggccgg tggttctggg tcgagcgggc gttctgcgac atgccatggc acggggccgc
      241 ctccgccaag ggggtggtga tctggaacca cggcattcac gggacggtgg cgcaacacac
      301 cgcgtcggtg ccgccggtct tccggctctt gcaggcgcgc ggctgggacg tggtgaagat
      361 cgcgcgcaac aatctcgggg agaccaccgg cgagcaatcc ctcaaccggg cggtccggcg
      421 gacccaggag gagatcgccg cgcgccgccg cgagggatac gggaaggtcg tgctggccgg
      481 gcagtccttc ggcggctaca tcacgctgga tacggcggag tcgtccaggg acgtttacgg
      541 tgtcgtcgcg atggccccgg gcatccggtt cggcggcacg ggcaacctcg acacgtcggt
      601 gacggagcgg acgctgggcg ccctcaccgt cgatcggctg gcgctcgtgt tcccgcgcaa
      661 cgacacgctc ttcgggagcc aggtgcgcgg gccgggcgcg gcccgggtcc tggcgcgccg
      721 cggcggctcc ttcctgctgc tcgacgagac gctcgacatc gtcgaccacg gcggggggac
      781 caccgccaag ttcgcggtgc gatacgggcc gtgtctcgtc cgctacctga cgagccccga
      841 ggtggggaag ggcgtggtgg gctgccccga gagcctggcc gagcagcgcg gcgcggccga
      901 ggagctgctg ccccggctcc ccggcaccgt gaagctcgcg ccggcgtccg atcgcgcggc
      961 cgaggagggt ggcgcgctcc tgggcaaggc gtggtacggc atcctggagc cggccgcgga
     1021 ggtcgtgtcc ttcgccatcg tggatgtgga cggcgtgggc cggcgtgcga tgttcggcgt
     1081 cgcctcgggc gggcggcgcg gcgggctcta cccgcttgcc accggagagc cgggcgtgac
     1141 gttccgcgtg gcggaccggg gcaccgtcac cgtgaagggg acaacgctca cctggacggc
     1201 gacgggcggc cgggcctcgc acgcggcgaa gctccatccg ctggccgagc cctagcgctc
     1261 gcgccggccg cgccgcgccg gccgccgtcg ccttgacagg gccgagccgg ccgagcagta
     1321 tccgggcgtg agcggtccgg ccctggtcca cgtcgtcgtc agcgggctcg cgacgggctg
     1381 catctacgcg ctggtcgcgc tcagcctcgt catcgtctac aacgccaccc gcaccgtgaa
     1441 cttcgcccag ggggagatgc tgatggtctc cgcgttcgtg gcgtggtccg cccagcacac
     1501 cgccgggctc ccgctgctgg ccgcggtggc ctgcggggtg gcggcgggcg ggctcctcgc
     1561 gttcctggtg gagcgtgcga tcatccggcg cgcggtctcg gccacccact gggacgtgct
     1621 gatcatcacg ctcgggctct cgctcgcgct gcgggccgcg gccggcatcg tgtggacgaa
     1681 cgaggagctg ccgttccccg ccctgttctc gaaccggccc gtcgcgatcg gcccggtgcg
     1741 cgtcgcgccg gtgtccctgg gggtcatcgg ggcctcgctc gtcctgatgc tggccctcgt
     1801 cgcgctcttc cgcgccaccc gcttcgggcg ggccatgcgc gcggtcgcgc agaaccagac
     1861 cgcggcgcgg ctcatgggca tcagcgtgga gcgggtgtac tcgggcgcgt gggtgctcgc
     1921 ggcggtggtg ggcggggtgg ccggcgtgct catcgccccg gtgacgttcc tctcgaccaa
     1981 gatggggttg atcgcgatca acggcttcac cgccgcggtg ctgggcggct tcggctcgct
     2041 gcccggcgcg gtggcgggcg gcatgctcct cggggtgatc gagaacctgg ccccgctcta
     2101 cctgccgacc ggcatccgct actcggtgcc cttcctcgtg atgatcgcca tcctggtcgc
     2161 gcgcccgcgc ggcctcttcg gctcgctcga gcggcggaag gtctagtgcg cgctgcggcg
     2221 tgggtgctgg gcgggatggc cgcgctcggg tggccgctcg tggcgccgcg gtacttcgtg
     2281 ttcctggcca ccctggtggc ggtgaacgcg gtcgtcgcga tcgggctcaa cctgctctcg
     2341 ggctacacca accagctctc gttcggccac gccggcttcc tcgccatcgg ggcctacacc
     2401 gcggcgatcc tcaccctgcg ggcgcccgcg ctgccggtgc ccctgaccct gctcgcggcc
     2461 gggctcgtca ccgccctcgt cggcctggcg ctcggcatcc cgtgcctccg gctcgacggc
     2521 ctctacctgg ccatggccac gctcgccttc ggcttcgtgg tcacggaggc gatcacgaac
     2581 ctcgactggc tcacccgcgg caacgacggc ctgcgcgtgc cgctcgcgca tctcggcccg
     2641 tgggcgctcg acaccgaccg ctcgcgctac tacctggccc tcgcggtggc cgcggcgatg
     2701 atcctggccg cgctcaacgt ggcggccacc cgcaccggcc gcgccttcct cgccatccgc
     2761 gccagcgaga tcgcggccca ggcgagcggg atcaacgcgg ccgcgtacaa gacggtggcc
     2821 ttcgtgctct cggccttcta caccggggtg gcgggcgggc tcttcgcctt cgtggtgggc
     2881 ttcctctccc cggacgcgtt cgacgtgttc ctctcggtgg acttcctggt gatgatcatc
     2941 ctgggcgggc ttggctccgt ctggggtggg gtggcgggcg cggcgatcgt caccgtgctc
     3001 aacgactcgc tggccgggtt ccaggcctac cggccgctca tcttcggcgc gatcatgatc
     3061 gcgtgcatgc tgttcgcgcc cggcggcctg gccgcgatga caaggaggca gtcatgaggc
     3121 aacgctacgc ggtggtcgtg ttggtggcgg cgatggtcgt ggcgatgctc gggggcctgg
     3181 cgggccggcc cgcggtcgcg gccgagcccg gcgtgaccga caccgagatc ctgctcggcg
     3241 gctcgaactc cttctcgggt ccgctcgcct tcaccggcac ccagctcacc aaggtcggcg
     3301 tcgatctcta cttccgcgtg ctcaacgacg cgggcggggt ccacggacgc aagatccgca
     3361 ccgtctacta cgacgacggt taccggccgc aggacgcggt ggccaacacg aagaagctgg
     3421 tggagcagga cgggatcttc gcggtgatcg cgccccaggg ctcgccgccg gtggtggcca
     3481 cgctcgacta cctcgagcag ggcaaggtgc cgatgctgtt tccgttccag agctcgaccg
     3541 cgacccggaa caagaagtac gtcttcagcg ggctcgtgct gagcgatcgc cagtcgcgga
     3601 tgatgatcga ctacctggcc ggcgcgcgga ggttcaagcg gttcgccgcg ctctaccagg
     3661 acgacgagta cggcaagtct ttcctgaccg cgttcgaggc cgatctcggt cgccacagcc
     3721 tcaagctgac cgccaccgag ccggtgaagc gcggggtcac cgacgtgagc gcgcagatcg
     3781 ccaagctcca ggccgcgaag cccgaggtca ccttcctggt cctgactccc ggtccggccg
     3841 cccaggcgct caaggagcgg cagaagatcg gctggggcga taccttgatg gtctcgacgg
     3901 gcccgctgac cgacgagacc tacctgggcc tggccggcga cgcctccgac ggggtggagg
     3961 gactggccac cctgcccgat ccgctgcgcg ccgataaccc gggggtgaag ctctaccggg
     4021 accacctgca gaagtacttc ccgaagaacg agccgaaccg ctactcgctc tccggctacg
     4081 tggccggcat gctcttcgcc gagggcgcca agcgcgcggg gcgcaatctc acccgcgacg
     4141 ggctcgttac cgcgctcgag tcgctcaagg gcttcgacac cgggatcctg ccgccggtca
     4201 cgatcggggc cgaccacgac acccagaagc agggcttctg ggtgcgggtg gagaacgggc
     4261 gcttcaagcc gctctcggac tggccgaagt cggacccgtg acgcggccgc ccgcgccgct
     4321 gctcgacatc gaggagctct cgatccgctt cggcggcctc gccgcgctga gcgaggtgga
     4381 tctggccgtc ggggcggggg agatcttcgc gctcatcgga cccaacgggg ccggcaagac
     4441 gaccgtcttc aatatcgtga ccgggctcta ccgccccctc gccgggcacg tccgttt
//
LOCUS       DATMCV010000831         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_243681, whole genome shotgun sequence.
ACCESSION   DATMCV010000831 DATMCV010000000
VERSION     DATMCV010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243681"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VNU03_19120"
     CDS             <1..964
                     /locus_tag="VNU03_19120"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76641.1"
                     /translation="LVRNPDYFVKGRPYLDGVRYTVIVERGTRVAALQAGQIDVAYPG
                     ETTLAIAEQLKNAVPGMVFTETASNVSENLLLNTKKPPFDNIKVRRALSFAIDRRTYT
                     QTVHRGAAVVGASLAPKPWGVWGLLDRDLGQLPGYGGAAAGRAQAKKLLAEAGFGPSN
                     PLKVELVTRAIAIYLDFAGFVVSDLKQVGVEATLKQIDTAQWHPMVTRREFQIGANLT
                     GLGVDDPDANFYENFSCGSPRNYGDYCNEEVGRLIDQQSQEIDAQKRLALVWQIQKKL
                     EEDAARPTMGWRTDRFAHHPYVKNLMPNQVTYNCCRLQEVWLDK"
     gene            975..1703
                     /locus_tag="VNU03_19125"
     CDS             975..1703
                     /locus_tag="VNU03_19125"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWP76642.1"
                     /translation="MRVVFWNIRHGGGVRADAIARRIRTWGADAVALCEFRGTPPSGE
                     LARALAARGLVYQCTTADPVRPTVNGLLLASRWPLRRVRVATAPVEPGRWLLARIQSP
                     TPLVVGVMHVPNRVTGRKYPYLDAVLETARRTWPGPAVFLGDTNSGRIGLDEEVPAFN
                     RIEDGWIRGLHEAGWIDAFRHLRGDARAYTWYSPNGGNGFRIDQAFLNGALRPRLESF
                     RYDWGRRDRRSASDHAAMLLDLTG"
     gene            complement(1846..>2044)
                     /locus_tag="VNU03_19130"
     CDS             complement(1846..>2044)
                     /locus_tag="VNU03_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76643.1"
                     /translation="LQWTDQASKVFAGAARFKPHKIRLLRKAGRVAEAQTLTVDCALS
                     GTTELKRACQEANQTPAAAAR"
BASE COUNT          316 a          734 c          707 g          287 t
ORIGIN      
        1 gctggtgcgc aacccggact acttcgtgaa gggccgcccc tacctcgacg gcgtgcgtta
       61 caccgtgatc gtggagcgcg gcacgcgggt ggccgcgctg caggcgggcc agatcgacgt
      121 ggcctatccg ggggagacca cgctcgcgat cgccgagcag ctcaagaacg cggtcccggg
      181 catggtgttc accgagaccg cgagcaacgt cagcgagaac ctgctcctga acaccaagaa
      241 gccgcccttc gacaacatca aggtccggcg cgcgctcagc ttcgccatcg atcggcgcac
      301 ctatacccag accgtgcatc gcggcgccgc ggtcgtcggg gcctccctgg ctccgaagcc
      361 gtggggcgtg tgggggctcc tcgatcgaga cctgggccag ctcccgggct acggcggggc
      421 cgcggcgggc cgggcgcagg cgaagaagct cctggccgag gcgggcttcg ggccgtcgaa
      481 tccgctcaag gtcgagctgg tcacccgcgc gatcgcgatc tatctcgact tcgcgggctt
      541 cgtggtcagc gatctcaagc aggtcggggt ggaagccacc ttgaaacaga tcgacaccgc
      601 gcagtggcac ccgatggtga cccgccgcga gttccagatc ggggccaacc tcaccggcct
      661 cggggtggac gatcccgacg ccaacttcta cgagaacttc tcgtgcgggt ccccccgcaa
      721 ctacggcgac tactgcaacg aggaggtggg ccggctgatc gatcagcagt cgcaggagat
      781 cgacgcccag aagcggctgg cgctcgtctg gcagatccag aagaagctcg aggaggacgc
      841 ggcccggccc accatgggct ggcggaccga tcgcttcgcc caccatccct acgtcaagaa
      901 cctgatgccg aaccaggtga cctacaactg ctgtcgcctg caagaggtct ggctcgacaa
      961 ataggcaagt accgatgcgg gtcgtcttct ggaacatccg gcacgggggc ggcgtccgcg
     1021 ccgacgccat cgcgcggcgg atccggacgt ggggcgccga cgcggtggcg ctctgcgagt
     1081 ttcgcggcac cccgccgagc ggcgagctcg cccgcgccct cgccgcgcgc ggcctcgtct
     1141 accaatgcac caccgccgac ccggtgcgcc ccaccgtcaa cggcctgctg ctggcctcgc
     1201 ggtggccgct ccggcgcgtg cgcgtggcca ccgcgcccgt cgagcccggc cgctggctcc
     1261 tcgcgcgcat ccagtcaccg accccgctcg tggtcggggt gatgcacgtc cccaaccgcg
     1321 tgacggggcg caagtacccc tacctggacg cggtgctcga gaccgcgcgc cggacctggc
     1381 cggggcccgc cgtcttcctg ggcgacacca actcgggccg gatcgggctc gacgaggagg
     1441 tgccggcatt caaccggatc gaggatggct ggattcgcgg gctccacgag gcgggatgga
     1501 tcgacgcctt ccggcatctc cgcggggacg cgcgggccta cacctggtac tccccaaacg
     1561 ggggcaacgg gttccggatc gaccaggcgt tcctgaacgg cgcgctgcgg ccccgcctgg
     1621 agagcttccg ctacgactgg ggccgacgcg accgccgctc cgccagcgac cacgccgcga
     1681 tgctcctcga cctcaccggc tgaccctcgg cgccgcagac cagctcacgc atggcggcgg
     1741 ctcgagaagg ggtcagctgc gaggcggcgc ccgaaggccg ctcgcaaggg gtactccatg
     1801 tacgtcgagc gagcggccga gggcgccaac gaagcagatg gccccttatc gagccgccgc
     1861 cgccggcgtt tgattggcct cttggcaggc gcgcttgagc tcggtggtcc cgctgagcgc
     1921 gcagtccacc gtgagggtct gcgcttcggc cacccgcccg gccttgcgca ggagccggat
     1981 cttgtggggc ttgaagcgcg ccgcgcccgc gaagaccttc gaggcctgat cggtccactg
     2041 gagc
//
LOCUS       DATMCV010000832         3455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_510762, whole genome shotgun sequence.
ACCESSION   DATMCV010000832 DATMCV010000000
VERSION     DATMCV010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510762"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VNU03_19135"
     CDS             <1..1152
                     /locus_tag="VNU03_19135"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWP76644.1"
                     /translation="PGAISFAGGMPDSGLFPTEAFRRVLNRVIREEGPELLQYYPARG
                     YPPLREFLSGYLLRFGLEARPEEILIVNGSQQGFDLVARTLLDPGDFVAIEEPSYPRA
                     MQVFRSFGAQLLPVPMVNGGLDPEHLDRLLERQSPKLLYCQPSAHNPTGLTMRDDARK
                     RLLEVAAHHRLPIMEDGFDGSLFFGPRPPAPLKALDVSGLVVYVGTFSKILFPGLRLG
                     WVVAAPELIERLEVAKELADIHTSPLIQAAVYHFCRQRLLDRHQARVLREYGRRRAAL
                     LQALAKRMPPGVTWTDHEGGFSLLLTLPPGMDATLLLERAAPRGVVFTAGNAFFVDGG
                     GERMLRLSFSALPLAQIDEGIRRLAEIIRDVQRQPEPAAVAALPGVPIV"
     gene            1165..2328
                     /locus_tag="VNU03_19140"
     CDS             1165..2328
                     /locus_tag="VNU03_19140"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWP76645.1"
                     /translation="MNFHTLAARGGRDVPSASRPLTAPIYQTNVYVFEDMDTVESVWE
                     SKKPGFVYGRYGTGNHAMLEDLVAALEGAEAAVACASGMGATTALLLGLFAPGDHLVS
                     ARDLYGSTAAFLGDEGRRLGVETDFVDATDTAAILGALRPATRAVFVEAVSNPLLRLV
                     DVPALAPELARRGIDLIVDASMTSPAVFRPIEHGATMVMHSLTKFISGHGDVTGGLVA
                     GRAEPMGRVRNAMIRAGTNLGPFDAWLATRGARTLAVRMERQSATALTLAQGLERLPA
                     VSRVYYPGLASHPQHALARRLMPKLGGAMVSFDLRGGAPAVERFMARARLLEFAPSFG
                     DVATTWTYPARTSHRRVSDEQKAAMGIGAGLVRVSVGLEDPAELMADLEGAVA"
     gene            2325..2981
                     /locus_tag="VNU03_19145"
     CDS             2325..2981
                     /locus_tag="VNU03_19145"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWP76646.1"
                     /translation="MSAVGVAPAPTLVRPVTLEGRFVRLEPLTEAHAGALLAAAGGSR
                     ETYALTLVPGTAAEAAAYVETALAEQRATRSLPFVTVDRASGRVIGSTRFLNIEFWPW
                     PAGNHHQRGPERPDVVEIGATWLAAVAQRTPINTEAKLLMLTHAFDHWRVHRVSLMTD
                     ARNQRSRNAIVRLGARFDGILRATRPASDGAIRDTAAFSILEAEWPAVRANLEARLTQ
                     "
     gene            3014..>3455
                     /locus_tag="VNU03_19150"
     CDS             3014..>3455
                     /locus_tag="VNU03_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWP76647.1"
                     /translation="MDSGTPRLKVGLAEMLKGGVIMDVTNAEQAKIAEDAGAVAVMAL
                     ERVPSDIRKEGGVARMSPVTKIKEIQKSVTIPVMAKCRIGHFVEAQILESLGVDFIDE
                     SEVLTPADEHFHVDKFAFKVPFVCGCRDLGEALRRIGEGAAMIRT"
BASE COUNT          475 a         1233 c         1256 g          491 t
ORIGIN      
        1 ccgggcgcca tctccttcgc gggtggcatg ccggacagcg ggctgttccc gaccgaggca
       61 ttccggcgcg tgctcaaccg ggtgatccgc gaggagggac ccgagctgct gcagtactac
      121 ccggcgcgcg ggtacccgcc gctgcgggag ttcctctccg gctacctcct gcgcttcggc
      181 ctcgaggcac ggccggagga gatcctgatc gtcaacggct cgcagcaggg cttcgacctg
      241 gtggcgcgca cgctcctcga tccgggcgac ttcgtggcga tcgaggagcc gtcgtatccg
      301 cgggccatgc aggtcttccg atcgttcggg gcccagctcc tgccggtgcc catggtgaac
      361 ggcgggctcg atcccgagca cctggaccgg ctcctcgagc ggcagtcgcc caagctgctc
      421 tactgccagc cgagcgccca caaccccacc gggctgacca tgcgcgacga cgcgcgcaag
      481 cggctgctcg aggtggcggc gcaccaccgc ctgccgatca tggaggatgg cttcgacggc
      541 agcctgttct tcgggccacg gccgcccgcg ccgctcaagg ccctcgacgt ctcgggcctc
      601 gtcgtctacg tgggcacctt ctcgaagatc ctcttcccgg gcttgcggct cggctgggtc
      661 gtggccgcgc cggagctgat cgagcggctg gaggtcgcca aggagctggc cgacatccac
      721 acgagcccgc tcatccaggc cgcggtctac cacttctgcc ggcagcgcct cctcgaccgt
      781 caccaggcgc gcgtgctgcg cgagtacggc cgccggcgcg cggcgctgct gcaggccctc
      841 gccaagcgca tgccacccgg cgtgacgtgg accgatcacg agggcggctt ctccctgctg
      901 ctcacattgc cgcccggcat ggacgcgacg ctcctgctcg agcgggccgc cccgcgcgga
      961 gtcgtcttca ccgcgggcaa cgcgttcttc gtggacggcg gcggcgagcg gatgctgcgc
     1021 ctgtcgttct cggcgctgcc gctcgcccag atcgacgaag gcatccggcg gctggccgag
     1081 atcattcgcg acgtgcagcg tcagccggag ccggccgcgg tggccgcgct gcccggcgtg
     1141 ccgatcgtgt agaggagata ccgcgtgaac ttccacaccc tggccgcccg cggaggacgc
     1201 gacgtgccgt ccgcgagccg cccgctgacc gcccccatct accagaccaa cgtgtacgtc
     1261 ttcgaggaca tggacacggt ggagtcggtg tgggagagca agaagccggg cttcgtgtac
     1321 ggccgctacg gcaccggcaa ccacgccatg ctggaggacc tggtggccgc gctggagggc
     1381 gcggaggccg cggtcgcgtg cgcctccggc atgggagcca ccaccgcgct cctgctgggg
     1441 ctgttcgcgc ccggcgacca tctggtgtcg gcgcgggatc tctacggctc caccgcggcc
     1501 ttcctcggtg acgaggggcg gcggctcggg gtcgagaccg atttcgtgga cgcgaccgac
     1561 accgcggcga tcctcggcgc gctgcgaccg gccacccggg cggtcttcgt ggaggcggtg
     1621 tcgaacccgc tgctgcggct cgtcgacgtg cccgcgctgg cgcccgagct ggcccggcgg
     1681 gggatcgatc tgatcgtgga cgcgtcgatg acgtcgcccg cggtgttccg cccgatcgag
     1741 cacggcgcca ccatggtgat gcacagcctg accaagttca tctcgggcca cggcgacgtc
     1801 accggagggc tcgtggcggg ccgggccgag ccgatggggc gcgtccgcaa cgcgatgatc
     1861 cgggccggca ccaacctggg cccgttcgac gcctggctcg cgacccgggg ggcgcgcacc
     1921 ctcgcggtgc ggatggagcg gcagagcgcg accgcgctga cgctggccca gggcctggag
     1981 cgcctgcccg cggtgtcgcg cgtgtactat cccggcctgg cgtcgcatcc gcagcacgcg
     2041 ctcgcgcggc gcctgatgcc gaagctcggg ggggcgatgg tgtcgttcga cctgcgcggc
     2101 ggcgcgcccg cggtggagcg cttcatggcg cgcgcccgcc tgctcgagtt cgcgccgagc
     2161 ttcggcgacg tggccaccac ctggacctat cccgcgcgca cctcgcaccg gcgcgtgtcc
     2221 gacgagcaga aggccgcgat ggggatcgga gccgggctgg tccgggtgtc ggtcgggctc
     2281 gaggatcccg ccgagctgat ggccgatctc gagggcgcgg tcgcatgagc gcggtggggg
     2341 tggcgcccgc gccgacgctg gtgaggccgg tgaccctcga gggccgcttc gtccggctcg
     2401 agccgctgac ggaggcgcac gcgggcgcgc tgctcgcggc cgcgggcggc tcgcgcgaga
     2461 cctacgccct caccctggtg ccgggcaccg cggcggaggc ggcggcctac gtcgagaccg
     2521 cgctcgccga gcagcgggcc acccgctcgc tgccgttcgt caccgtggac cgcgcgagcg
     2581 ggcgggtgat cggctcgacc cgcttcctca acatcgagtt ctggccgtgg cccgcgggca
     2641 atcaccacca gcggggaccg gagcgccccg acgtggtgga gatcggcgcg acgtggctgg
     2701 ccgccgtggc ccagcgcacc ccgatcaaca cggaggcgaa gctcctgatg ctgactcacg
     2761 cgttcgacca ctggcgcgtc caccgcgtga gcctgatgac cgacgcgcgc aaccagcgct
     2821 cgcggaacgc catcgtgcgg ctgggcgccc gcttcgacgg aatcttgaga gcgacgcggc
     2881 ccgccagcga cggggccatt cgcgacaccg ccgccttctc gatcctggag gcggagtggc
     2941 ccgcggtccg ggccaacctg gaagcacgac tcacgcagtg atccgatcca cactcgaaaa
     3001 aggagacgag gacatggaca gcggaacccc gagactgaag gtgggactgg ccgagatgct
     3061 gaagggcggc gtcatcatgg acgtgaccaa cgccgagcag gccaagatcg cggaggacgc
     3121 gggcgcggtc gcggtgatgg cgctcgagcg cgtgccgtcc gacatccgca aggaaggcgg
     3181 agtggcccgc atgtcgccgg tcaccaagat caaggagatc cagaagtcgg tcaccatccc
     3241 ggtgatggcc aagtgccgca tcggccactt cgtggaggcg cagatcctcg agtcgctcgg
     3301 tgtcgacttc atcgacgagt ccgaggtgct gaccccggcc gacgagcact tccacgtgga
     3361 caagttcgcc ttcaaggtgc ccttcgtctg cggctgtcgc gacctgggcg aggcgctccg
     3421 gcgcatcggc gaaggggcgg cgatgatccg cacga
//
LOCUS       DATMCV010000833         5282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_840824, whole genome shotgun sequence.
ACCESSION   DATMCV010000833 DATMCV010000000
VERSION     DATMCV010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840824"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VNU03_19155"
     CDS             complement(<1..572)
                     /locus_tag="VNU03_19155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76648.1"
                     /translation="MAADGPGEILLELVDVHAGYGEIEVLRGVSADVRAGEIVSIIGA
                     NGAGKSTLLRTVFGMVTPSRGRIRLAGEEIAGENPVAVLRRGCSYVPQGRCNFPAMSV
                     LENLEMGAYTRSDASVRRDIEAMLERFPMLGAKRREPAGTLSGGQQQILEIAIALLLH
                     PRVLLIDEPSLGLDPRMVEIVFSTIVTVNRA"
     gene            complement(562..1317)
                     /locus_tag="VNU03_19160"
     CDS             complement(562..1317)
                     /locus_tag="VNU03_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76649.1"
                     /translation="MALFETRGLTKRFLGVTAVDAVDLTVEAGELVSLIGPNGSGKTT
                     LFNCVTGYLAPDEGRVTFQGRDVTGVAAHRIARLGIGRTFQLVSVFPRLSALENLLTF
                     LQQHQEESLVGRLVRAPRVRRLEAAAVERARSLLDSVGLGGRAEAPAGTLSYGQRKLL
                     AFAAALMPDPDLLLLDEPAAAVNPTMIEQMKAQIRALHRQGKTVLLVEHNMDVVMDIS
                     QRVIVLDHGQKIAEGPPEVVRRDPKVVEAYFGR"
     gene            complement(1320..2285)
                     /locus_tag="VNU03_19165"
     CDS             complement(1320..2285)
                     /locus_tag="VNU03_19165"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76650.1"
                     /translation="MRATRWVLGSAALVAVPWIAAAVVPGQQRYVLHVLIFTALYGAL
                     ALSYDLVVGHVGSLSLAHPAFFGLGAYTLALLATEARWPFAFALPAGIALAAVVALAV
                     GVPMFRLTEHSFAMGTLGFAIVTQVVATNWVDFTHGPLCVTGIPKPQVGALRIATLPA
                     FYWMGLGALALVALLYRGLTTFRLGRAFHAVRDNETLAAAASIDPLRYRMLAFVIGGG
                     VAGGIGALYVSYVGVLCPGELTVSLSVNLLVMVFLGGVGSLRGVLVGAAAFTALPEIL
                     RMAPTWRMVIYGLLLLVIVIRAPDGLEGLMRRASSRGRRPSASGA"
     gene            complement(2288..3157)
                     /locus_tag="VNU03_19170"
     CDS             complement(2288..3157)
                     /locus_tag="VNU03_19170"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76651.1"
                     /translation="MSDLLEQLVHGLTLGSLYAMVATGLALMFGVVRLINFAHGEFYM
                     LGGYAFWYAYVELGLPYPVAGLAAVAAMAVFGLAYERAVIRTILARTWHVQLIATLAT
                     SITLTNLAIIVFGTQPKEVPTRLSSVILDVGGVRMAWQRVLVLVAAIAIFVALEWFVA
                     NAKLGKAMRAMSQNREACAVVGVDVQQVALATFGISAGLAAAAAALVSPLFSIFPDVG
                     ALLTLKAFAAVIVGGFGYVRGAIAAAFLIGIAESLAAGYLSYAYKDAIAFVVMVAVLL
                     VRPQGLFGRRIGI"
     gene            complement(3165..4343)
                     /locus_tag="VNU03_19175"
     CDS             complement(3165..4343)
                     /locus_tag="VNU03_19175"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76652.1"
                     /translation="MTRTGYRAIGVAIAVGLAWGLAAEAPPAGAQTAPPIRVGFSSAM
                     TGPSAITGEGVKWAAQMLADEYNAKGGIAGRKIELYFGDNAGTPGEAVSAVRKLVDVD
                     KVDVIIGQTHSGACLGALPVVKELQVPMVIEACSHPKIRDQIGKNGNEWAFRVNPDDV
                     MLANQFAKYMVQQAKTVSIFAQNDDFGRGAAAAYDAAFKKVGIKLLSTEFFDRGQADY
                     RPVLTRVKRAAPEGVLLVMLASEGSVFMRQYRELGLNQKLFARGSMGTVEFLYQVRDS
                     PNIADGLVEATYWTPALDPAWEKQWLERWKVPVRIHGSLAAIAFRYAVAPAIEQAIKK
                     TGKADRKAIRDALEEVDVPNTPVGRIKFDDTHQAFINMLLVEINGGQLKVLEKIPIQP
                     "
     gene            complement(4364..>5282)
                     /locus_tag="VNU03_19180"
     CDS             complement(4364..>5282)
                     /locus_tag="VNU03_19180"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWP76653.1"
                     /translation="NYTDDPGPIATRVREITSRPAAGPGEALREILDGFGVAAGRLAV
                     DEAGVFPATWQRLGEQLPGATISQAYAHFRTARMVKSPEEVRRLERAARIAEDGIAAI
                     FGMLKPGVTEREAASAFEQEVIRRGAQTFFTVVTIGPHRAALADVYASETALKAGDLI
                     RLDLGCVYQGYRSDISRTALLGTPTEKQRRYYDAIRDGEKAAIAAMKPGVAVSQIFEI
                     GMKVTREQGMPHYQRNHVGHGIGLEPYDPPTINATGQTVLEPGMVFCVETPYYEHGWG
                     GIQVEDAVEITAAGVRRLTQSSQELQILG"
BASE COUNT          811 a         1932 c         1808 g          731 t
ORIGIN      
        1 gcccggttga ccgtcacgat ggtcgagaac acgatctcca ccatccgggg atcgagcccg
       61 agcgagggct cgtcgatcag caaaacgcgc gggtggagca gcagcgcgat cgcgatctcg
      121 agaatctgct gctggccgcc cgacagggtg ccggcgggct cccggcgctt ggcgcccagc
      181 atcgggaacc gctccagcat cgcctcgatg tcccggcgaa cggaggcgtc cgaccgggtg
      241 taggcgccca tctcgaggtt ctccaggacg ctcatcgcgg ggaagttgca ccgtccctgc
      301 ggcacgtagg agcagccgcg acgcaggacg gccaccggat tctcccccgc gatctcctcg
      361 ccggccagcc ggatgcggcc acgcgagggg gtgaccatgc cgaacacggt gcggagcagc
      421 gtggacttcc ccgccccgtt ggccccgatg atcgacacga tctcgcccgc ccgcacgtcg
      481 gccgagacgc cgcgcagcac ctcgatctcg ccgtacccgg cgtgcacgtc cacgagctcc
      541 agcaggatct cgccgggccc gtcagcggcc aaagtacgcc tccaccacct tcggatcccg
      601 gcggacgacc tcgggcgggc cttccgcgat cttctgcccg tggtcgagca cgatcacccg
      661 ctgggagatg tccatcacca cgtccatgtt gtgctccacc agcaacaccg tcttgccctg
      721 ccggtggagc gcccggatct gggccttcat ctgctcgatc atcgtcgggt tcaccgcggc
      781 cgccggctcg tcgagcagca gcaggtccgg atccggcatc agggccgccg cgaaggcgag
      841 cagcttccgc tggccgtagg agagcgtgcc cgcgggcgcc tcggcccgcc cgcccaggcc
      901 caccgagtcg aggaggctcc gcgcccgctc caccgccgcc gcctccagac gtcgcacccg
      961 cggcgcgcgc accagccggc cgaccaggct ctcttcctgg tgctgctgca ggaacgtcag
     1021 caggttctcg agcgcggaga ggcggggaaa gacgctgacg agctggaagg tgcgaccgat
     1081 gccgagccgg gcgatgcggt gagcggcgac cccggtcacg tcgcggccct ggaaggtcac
     1141 ccgaccttcg tccggcgcca ggtagccggt cacgcagttg aagagcgtgg tcttgcccga
     1201 gccgttgggg ccgatgaggc tcaccagctc ccccgcctcg acggtgagat cgaccgcgtc
     1261 gaccgcggtg acaccgagga agcgcttggt gagcccgcgc gtctcgaaga gcgccatcgc
     1321 tacgctcctg acgcggacgg gcgccggccg cgcgacgagg cccggcgcat caggccctcg
     1381 agcccgtcgg gcgcgcggat cacgatcacc agcagcagca gcccgtagat caccatgcgc
     1441 caggtcgggg ccatgcggag gatctcgggc agcgcggtga aggcggcggc gcccaccagg
     1501 acgccccgca ggctccccac cccgccgagg aagaccatga ccagcaggtt gacgctcagg
     1561 ctgacggtca gctctcccgg gcagagcacg cccacgtagg agacgtagag cgcgccgatc
     1621 ccgcccgcca cgccaccccc gatcacgaag gcgagcatgc ggtaccggag cggatcgatc
     1681 gacgcggcgg cggccagggt ctcgttgtcc cgcaccgcgt ggaaggcccg cccgagccgg
     1741 aacgtggtga gcccgcgata gaggagagcg accagggcca gggcgccaag ccccatccag
     1801 taaaacgcgg ggagcgtggc gatccggagc gcgcccacct gcggcttggg gatgccggtc
     1861 acgcagagcg gcccgtgggt gaagtccacc cagttggtgg ccacgacctg ggtcacgatc
     1921 gcgaagccga gcgtccccat cgcgaagctg tgctcggtca gccggaacat gggcacgccc
     1981 accgcgagcg cgaccaccgc ggccagcgcg atgccggcgg gcagcgcgaa cgcgaagggc
     2041 cagcgggcct cggtggcgag gagggccagc gtgtaggcgc cgaggccgaa gaaggcgggg
     2101 tgggccagcg agaggctccc cacgtggccc accaccaggt cgtagctcag cgccagcgcc
     2161 ccgtagagcg cggtgaagat cagcacgtgg aggacgtagc gctgctggcc gggcaccacc
     2221 gccgcggcga tccacgggac cgcgaccagc gcggccgagc ccagcaccca ccgggtggcg
     2281 cgcacgatca gatcccgatc cgccggccga agagcccctg cggccgtacc agcagcaccg
     2341 ccaccatgac cacgaacgcg atcgcgtcct tgtacgcgta agagaggtag cccgccgcga
     2401 gcgactccgc gatgccgatc aggaaggcgg ccgcgatggc cccccgcacg tagccgaagc
     2461 cgcccacgat gaccgccgcg aaggccttca gggtgagcag ggcgcccacg tcggggaaga
     2521 tgctgaagag cggggagacc agggcggccg ccgcggcggc cagccccgcc gagatgccga
     2581 aggtggcgag cgcgacctgc tggacgtcga ccccgaccac cgcgcacgct tcgcggttct
     2641 gcgacatggc gcgcatcgcc ttccccagct tggcgttggc cacgaaccac tcgagggcga
     2701 cgaagatcgc gattgccgcg accagcacga gcacccgctg ccacgccatc cgcaccccgc
     2761 ccacgtcgag gatgacggac gagagccgcg tcggcacctc cttgggctgg gtgccgaaca
     2821 cgatgatcgc caggttcgtg agcgtgatcg aggtggccag cgtggcgatg agctgcacgt
     2881 gccaggtccg ggccaggatc gtgcggatca ccgcccgctc gtaggcgagc ccgaagaccg
     2941 ccatcgcggc caccgcggcg aggccggcca ccggatacgg cagccccagc tccacgtacg
     3001 cgtaccagaa cgcgtagccc cccagcatgt agaactcgcc gtgggcgaag ttgatgaggc
     3061 gcaccacccc gaacatgagg gccaggccgg tggccaccat cgcgtagagc gatcccaggg
     3121 tgaggccgtg gacgagctgc tcgaggagat cgctcaagcg cgcgctacgg ctggatcggg
     3181 atcttctcca gcaccttgag ctggccgccg ttgatctcga ccaggagcat gttgatgaac
     3241 gcctggtggg tgtcgtcgaa cttgatccgc cccaccgggg tgttcggcac gtccacctcc
     3301 tcgagcgcgt cgcggatcgc cttgcgatcg gctttccccg tcttcttgat cgcctgctcg
     3361 atggcgggcg ccaccgcgta gcggaacgcg atggcggcga ggctgccgtg aatgcgcacc
     3421 ggaaccttcc agcgctcgag ccactgcttc tcccacgcgg gatcgagggc gggcgtccag
     3481 taggtggcct ccaccaggcc gtcggcgatg ttggggctgt cccgcacctg gtacaggaac
     3541 tcgaccgttc ccatgctccc ccgcgcgaag agcttctgat tgaggccgag ctcccggtac
     3601 tggcgcatga acacgctgcc ctcgctcgcg agcatcacca gcagcacccc ctccggggcc
     3661 gcgcgcttca cccgggtgag caccggccgg tagtcggcct ggccgcggtc gaagaactcc
     3721 gtcgacagca gcttgatgcc gaccttcttg aacgcggcgt cgtaggccgc ggcggccccg
     3781 cgcccgaagt cgtcgttctg ggcgaagatc gacaccgtct tggcctgctg gaccatgtac
     3841 ttggcgaact ggttggcgag catcacgtcg tcggggttca cccggaacgc ccactcgttg
     3901 ccgttcttgc cgatctggtc gcggatcttg ggatggctgc acgcctcgat caccatcggc
     3961 acctgcagct ccttcaccac cggcagcgcc ccgaggcacg ccccgctgtg ggtctggccg
     4021 atgatgacgt ccaccttgtc gacgtcgacc agcttgcgca ccgcgctcac cgcctcgccg
     4081 ggggtgcccg cgttgtcgcc gaagtagagc tcgatcttgc ggcccgcgat cccgcccttg
     4141 gcgttgtact cgtcggcgag catctgggcc gcccacttga cgccctcgcc ggtgatggcg
     4201 gagggcccgg tcatggccga ggagaacccc acccggatcg gcggcgcggt ctgcgccccc
     4261 gccggcggcg cctccgcggc gagcccccag gccagcccca ctgcgattgc gaccccgatt
     4321 gcccggtatc cggtgcgtgt catcgcgctc tcctcgctcg gcctcagccg aggatctgca
     4381 gttcctggct cgactgggtg agccgccgga ctccggcggc ggtgatctcg accgcgtcct
     4441 ccacctggat cccgccccac ccgtgctcgt agtacggggt ctccacgcag aagaccatgc
     4501 ccggctccag caccgtctgc cccgtcgcat tgatggtggg cgggtcgtag ggctccagcc
     4561 cgatgccgtg gccgacgtgg ttgcgctggt agtggggcat gccctgctcg cgggtcacct
     4621 tcatgccgat ctcgaagatc tggctcaccg ccacccccgg cttcatggcc gcgatggccg
     4681 ccttctcgcc gtcgcggatc gcgtcgtagt agcggcgctg cttctcggtg ggcgtgccca
     4741 gcaaagcggt gcgcgagatg tccgagcggt agccctggta cacgcagccg aggtcgaggc
     4801 ggatcaggtc ccccgccttc agcgcggtct cggacgcgta cacgtcggcc agcgcggccc
     4861 ggtggggacc gatggtcacc accgtgaaga acgtctgggc tccccggcgg atcacctcct
     4921 gctcgaaggc gctggccgcc tcgcgctcgg tgacgcccgg cttcagcatg ccgaagatcg
     4981 ccgcgatgcc atcctccgcg atccgcgcgg ccctctccag gcgccgcacc tcctccgggc
     5041 tcttgaccat ccgagccgtc cggaagtggg cgtacgcctg ggagatggtg gcgccgggga
     5101 gctgctcgcc gaggcgctgc caggtggccg ggaacacgcc ggcctcgtcc accgcgagac
     5161 gcccggccgc gacgccgaag ccgtccagga tctcgcgcag cgcctccccg gggcccgcgg
     5221 cgggccggct cgtgatctcg cgcacccgcg tggcgatcgg gcccggatcg tcagtgtagt
     5281 tg
//
LOCUS       DATMCV010000834         7066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_43391, whole genome shotgun sequence.
ACCESSION   DATMCV010000834 DATMCV010000000
VERSION     DATMCV010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43391"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /gene="polA"
                     /locus_tag="VNU03_19185"
     CDS             <1..1611
                     /gene="polA"
                     /locus_tag="VNU03_19185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWP76654.1"
                     /translation="IESVSEGGPPDPTITAVGLYHPAAGAARVAVASPADEPGRALVA
                     ALGGRTLIGHDAKALAEWWLARGGALPPGEDTAVAAYLLNPARTNYKLEEVCAELLGE
                     GPGIAPPGTRGKWIWDLWAMAPRALAEVGLASLYEDIERPLIGVLAEMERHGIRIDQV
                     RLGEFSRELEVHLERTTREIFGLAGEEFNIGSPKQLAYILFEKLKLPPVRRTKTGYST
                     DADVLEQLALGHELPARIIEHRTLAKLKSTYADALPMLVNPTTGRLHTSFNQLVAATG
                     RLSSSAPNLQNIPVRTELGRRIRAAFVPDAGWRFVAADYSQIELRILAHVSGEESLIE
                     AFRRGEDIHARTASEVFGVALDAVTPEQRDIAKTTNFAVIYGVTAFGLSRGLDMSTKQ
                     AQEFLDRFFARHPKVKAYLGRTVAEGRERGFVATLLGRRRYLPELRSGNPNLRGFGER
                     MATNAPIQGTAADLVKIAMVRMARELRAQGLESRMLLQVHDELLFETPAAEVGRLQAL
                     ATEVMESALALDVPLKVDVKVGDDWAAV"
     gene            1608..2243
                     /gene="coaE"
                     /locus_tag="VNU03_19190"
     CDS             1608..2243
                     /gene="coaE"
                     /locus_tag="VNU03_19190"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827869.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWP76655.1"
                     /translation="MRRFLLVGLTGGIATGKSTVTAMLANPSVRVVDADALAREVVEP
                     GTPAHRQIVAEFGPDVLQADGRLDRARLGEIVFPDPVKRKRLEAITHPAIRGRFEAIM
                     ADLERKGWDGILIWDAALLVESGGNKNMDRMVVVTTDPATQLRRLVARDGCTEEAARA
                     RTASQMPLAVKARYADYVIDNSGPREQTEARVREVYRALLDDLRQRPTARR"
     gene            2240..3118
                     /gene="rsmA"
                     /locus_tag="VNU03_19195"
     CDS             2240..3118
                     /gene="rsmA"
                     /locus_tag="VNU03_19195"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWP76656.1"
                     /translation="MSPRRNARAAAPRAPVYRSATRGRTRALGQHFLRDRTVVDRILE
                     LVRPTARDLAVEIGPGRGALTEALAARAGRLLALEIDAALAGALQERFAGVGSVEIRQ
                     ADARLFDYAGLRAGAPDPGGRVLVVGNLPYSVGKPILAALVESGGAIDEMALMLQKEV
                     AERVAAQPGSKTYGALSVLTQVSAAVRLAFTVPPGAFSPPPQVDSAVLYLRLLREPPV
                     PVADPARFGAVVRAAFSQRRKSLANALAAGLAVSAESARRQAEAAGIDPGRRAETLSL
                     AEFARLAAQIAHPDPA"
     gene            3173..4876
                     /locus_tag="VNU03_19200"
     CDS             3173..4876
                     /locus_tag="VNU03_19200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWP76657.1"
                     /translation="MNTTYRPEPVPIDLPGEPAVRLIPLGGLGEIGLNMMLIESGRDI
                     VAVDCGLLFPDDEMPGVDFAIPDFSYLRERRDAFRAVVLTHGHEDHIGALSYLLRDFP
                     VPVYGTPLTLAIARHRLAENGLLEQADLRAYAPGDRLVIGGFTVEPIRVTHSIADGIG
                     LAIETPLGTVVHTGDFKLDRQPIDHQQPDYSRFAALGEQGVLVLCSDSTNVGRPGHTG
                     SESLVGEALGGRFAAAPGRIIVATFASHIHRIQQVLDLAARGDRHVALLGRSMVANVA
                     VAAELGYLNVPEGLVLPLEDLAELPAQRQVILSTGSQGEPNSAISLIAAGDHKYVQVG
                     PGDLVIFSSRVIPGNERVLGRMINSLLRRGTEVLWEDVAFVHVSGHASQEDLKEMLAL
                     TRPRYFVPVHGEFRHLLQHARLAEGVGIPKDRIFLIEDGLGLEVTASGTRVLGPYPAG
                     PVLVDGKGVGDVGGVVLRDRQLLAEAGMLVVALTVDRTTGSIVAGPEIASRGFVYMKE
                     ADALMDEVKTAVRDALAARREPAGFDREQLGATVRSAVRRFVNQRFQRKPVVLPVILE
                     V"
     gene            4876..>7066
                     /locus_tag="VNU03_19205"
     CDS             4876..>7066
                     /locus_tag="VNU03_19205"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWP76658.1"
                     /translation="MAEKVTIEKVLPDRTPGPNGHGPRESREASRPGALKRSGPRKSK
                     GEPGRWVREAKGILALALAGFGFVALYAYDPTLHPLDQSSPVGPVGGWLGWGSFHLFG
                     YAGYLFPVLFTLYGVSAFVRPRMAHGWQAGAGLAILLVSATGILARASDTLAEFRVHK
                     GGVVGWAVSEALRLSIGTVGTWIILLALIPLGVLFVTRIPYSVLSRGLRARFGRLRRA
                     EAKKGRSQSPASPGAPVIAAALAGLPAIEADVPPRLVVKEPPKAKSSLSDKGLAWQET
                     FDFGKGGAESFKLPAVGLLAPPPASSLQRTREELETNAQTLKKKLHDFGVDGHIVQAS
                     PGPVITSYEFEPASGVKVSQVVNLADDLALAMKVAAVRIIGPIPGRGTVAVEVPNPET
                     TTVYLREILVSQEFVTNKARLPLALGKDTTGNSVIADLAAMPHLLIAGSTGSGKSVGL
                     NTMICSLLYKATPADVRFLLIDPKRLELGVYEKIPHLLAPVVTDAKEASARLKWIVGK
                     MDARYKQLQAKAVRNIEGYNKEVPPEERLPYWVVVVDELADLMMVSAGEVQNSLIRLA
                     QIARAVGIHLIVATQRPSVDVVTGLIKANFPTRIAFQVASKVDSRTVLDANGAEQLLG
                     RGDMIFVPPGQSRQMRVHGSWVSDKEVKAICEFLRTQGSAVYEHVELPSEEAGYGGGG
                     EDRDDIYWDAVNLVIAQRAASISFLQRRLGLGYPKAARFIDMMEQDHV"
BASE COUNT          970 a         2519 c         2594 g          983 t
ORIGIN      
        1 atcgagagcg tcagcgaggg cgggccgccc gatcccacca tcaccgcggt cgggctctac
       61 catccggcgg cgggggcggc ccgggtcgcg gtggcgtcgc ccgcggacga gccgggccgc
      121 gcgctggtgg cggcgctcgg cggccggacc ctgatcggcc acgacgcgaa ggcgctcgcc
      181 gagtggtggc tggcgcgcgg cggcgcgctg ccgcccggcg aggacaccgc ggtcgccgcg
      241 tacctgctca atcccgcgcg cacgaactac aagctcgagg aggtgtgcgc cgagctcctg
      301 ggcgaagggc cgggcatcgc gccgccgggc acgcgcggca agtggatctg ggatctctgg
      361 gcgatggcgc cgcgcgcgct cgcggaggtg gggctcgcga gcctctacga ggacatcgag
      421 cggcccctga tcggggtgct ggccgagatg gagcgccacg gcatccggat cgaccaggtc
      481 cggctcggcg agttctcccg cgagctcgag gtgcacctcg agcggacgac ccgggagatc
      541 ttcgggctcg cgggcgagga gttcaacatc ggctcgccga agcagctcgc ctacatcctg
      601 ttcgagaagc tcaagctgcc gccggtgagg cggaccaaga cgggctactc caccgacgcc
      661 gacgtgctcg agcagctggc gctgggccac gagctgcccg cgcgcatcat cgagcaccgc
      721 accctggcca agctcaagtc gacgtacgcc gacgcgctgc ccatgctggt gaaccccacc
      781 accgggcgcc tgcacacctc gttcaaccag ctggtggccg cgaccggacg cttgagttct
      841 tcggcgccga accttcagaa catacccgtg cgcaccgagc tcggccggcg catccgcgcc
      901 gccttcgtgc ccgacgcggg ctggcgattc gtggccgcgg actactccca gatcgagctg
      961 cgcatcctgg cccacgtctc gggggaggag agcctgatcg aggccttccg ccgtggggag
     1021 gacatccacg cccgcaccgc ctccgaggtc ttcggggtcg cgctcgacgc ggtcaccccc
     1081 gagcagcgcg acatcgccaa gaccaccaac ttcgcggtga tctacggggt caccgccttc
     1141 gggctctccc gcggcctcga catgagcacc aagcaggccc aggagttcct cgatcgcttc
     1201 ttcgcgcggc acccgaaggt gaaggcctac ctcggtcgca ccgtggcgga ggggcgcgag
     1261 cgcgggttcg tggccaccct gctcggccgc cgccgctacc tgcccgagct gcggagcggc
     1321 aaccccaacc tgcgcggctt cggcgagcgc atggccacca acgcgcccat ccagggcacc
     1381 gcggcggacc tggtgaagat cgcgatggtc cggatggcgc gcgagctgcg cgcgcagggg
     1441 ctcgagagcc gcatgctgct gcaggtgcac gacgagctgc tcttcgagac gcccgcggcg
     1501 gaggtggggc ggctccaggc gctcgcgacc gaggtgatgg aatcggcgct cgccctggac
     1561 gtgccgctga aggtcgacgt caaggtcggc gacgactggg ccgcggtgtg aggcgcttcc
     1621 tgctggtcgg cctcaccggc gggatcgcca ccggcaagag caccgtcacc gcgatgctgg
     1681 ccaacccgag cgtgcgggtg gtggacgcgg acgcgctcgc ccgcgaggtg gtggagcccg
     1741 ggaccccggc ccaccggcag atcgtggccg agttcggccc ggacgtgctc caggccgacg
     1801 gccggctcga ccgcgcgcgc ctcggcgaga tcgtgtttcc ggacccggtc aagcgcaagc
     1861 gcctcgaggc gatcacgcac cccgcgatcc gcgggcgctt cgaggccatc atggcggacc
     1921 tcgagcggaa gggatgggac ggcatcctga tctgggacgc ggcgctgctg gtggagtccg
     1981 gcggcaacaa gaacatggac cggatggtgg tggtgaccac cgatccggcc acgcagctcc
     2041 gccggctggt ggcccgcgac ggctgcaccg aggaggcggc gcgggcccgc accgcgagcc
     2101 agatgccgct cgcggtcaag gcccgctacg ccgactacgt catcgacaac tcgggcccgc
     2161 gcgagcagac ggaggcgcgc gtgcgcgagg tctaccgcgc cctcctcgac gacctgcgcc
     2221 agcgccccac ggcccgccgg tgagcccgcg ccggaacgcg cgcgccgcgg cgccgcgcgc
     2281 gccggtgtac cggtcggcga cccgtgggcg cacccgggcc ctcggccagc acttcctgcg
     2341 ggatcgcacc gtggtcgacc gcatcctgga gctggtgcgg ccgaccgcgc gcgacctggc
     2401 ggtcgagatc ggccccgggc gcggcgcgct caccgaggcg ctggccgcgc gcgccgggcg
     2461 gctgctggcc ctcgagatcg acgcggcgct ggccggagca ctgcaggagc gcttcgcggg
     2521 cgtgggctcg gtcgagatcc gccaggccga cgcgcggctg ttcgactacg cgggtctgcg
     2581 cgccggagcc cccgatcccg gcggccgcgt gctcgtcgtg ggcaacctgc cctacagcgt
     2641 gggcaagccg atcctggccg ccctggtcga gagcggcggg gcgatcgacg agatggcgct
     2701 gatgctccag aaggaagtcg ccgagcgggt ggcggcccag ccggggagca agacctacgg
     2761 cgcgctctcg gtgctgaccc aggtgtcggc cgcggtgcgg ctcgccttca cggtgccgcc
     2821 gggggccttc agccccccgc cgcaggtgga ctcggcggtg ctgtacctgc gcctgcttcg
     2881 cgagccgccg gtgccggtcg ccgatccggc ccgattcggg gcggtggtgc gcgccgcgtt
     2941 cagccagcgc cgcaagagcc tggccaatgc cttggccgcg gggctggccg tctccgccga
     3001 gagcgcccgg cgccaggcgg aggcggcggg catcgacccg ggccgccggg ccgagacatt
     3061 atcgctcgcc gagttcgccc ggctggccgc tcagatcgct catcccgatc cggcctagcc
     3121 tccccgttcc ttgggccgga cgggggcccg gtggtaggat gaaaaggccg tcgtgaacac
     3181 cacttacaga ccagagcccg ttcccatcga cctccccggc gagcccgcgg ttcggctcat
     3241 cccgctgggc gggctgggcg agatcgggct caacatgatg ctgatcgagt ccggccggga
     3301 catcgtcgcg gtggactgcg ggctgctctt cccggacgac gagatgccgg gcgtggactt
     3361 cgcgatcccg gacttctcct atctccggga gcggcgcgac gcgttccgcg cggtggtgct
     3421 cacccacggt cacgaggatc acatcggcgc gctctcctac ctgctccggg acttcccggt
     3481 gccggtctac gggacgccgc tcaccctggc gatcgcgcgc caccggctcg ccgagaacgg
     3541 cctgctcgag caggccgacc tgcgcgccta cgccccgggg gaccggctcg tcatcggagg
     3601 cttcacggtc gagccgatcc gcgtgaccca ctcgatcgcc gacggcatcg gcctggccat
     3661 cgagacgccg ctcggcaccg tggtgcacac cggcgacttc aagctcgacc ggcagccgat
     3721 cgaccaccag cagccggact actcgcgctt cgcggcgctc ggcgagcagg gtgtgctcgt
     3781 gctctgctcc gattcgacca acgtggggcg ccccggccac accggctcgg agtccctggt
     3841 gggcgaggcg ctcggcgggc gcttcgcggc ggcgccgggc cgcatcatcg tggccacctt
     3901 cgcctcgcac atccatcgca tccagcaggt gctggacctg gccgcgcggg gcgatcgcca
     3961 cgtggccctc ctggggcgga gcatggtggc caacgtggcg gtggccgcgg agctcggtta
     4021 tctgaacgtg cccgagggcc tcgtgctgcc gctcgaggac ctggccgagc tgcccgccca
     4081 gcggcaggtg atcctctcca ccggcagcca gggcgagccc aactcggcca tctccctgat
     4141 cgcggcgggc gaccacaagt acgtgcaggt gggcccgggc gacctggtga tcttctcctc
     4201 gcgggtgatc ccgggcaacg agcgcgtgct cggccgcatg atcaactcgc tgctgcgccg
     4261 cgggaccgag gtgctgtggg aggacgtggc cttcgtccac gtctccggcc acgcgagcca
     4321 ggaggacctc aaggagatgc tcgcgctcac ccggccgcgc tacttcgtgc ccgtgcacgg
     4381 cgagtttcgc cacctgctgc agcacgcgcg gctggccgag ggcgtgggca ttcccaagga
     4441 ccgcatcttc ctgatcgagg acgggctcgg gctcgaggtg accgcgtccg gcacgcgcgt
     4501 gctggggccc tacccggcgg ggccggtgtt ggtggacggc aagggcgtgg gcgacgttgg
     4561 cggcgtggtg ctccgcgacc gtcagctcct ggccgaggcg ggcatgctgg tggtcgcgct
     4621 caccgtcgac cgcaccaccg gctcgatcgt ggcgggaccc gagatcgcct cccgcgggtt
     4681 cgtctacatg aaggaggcgg acgcgctgat ggacgaggtc aagaccgcgg tgcgggacgc
     4741 gctggccgcg cggcgggagc cggcggggtt cgaccgggag cagctcgggg ccacggtgcg
     4801 aagcgcggtg cgccgcttcg tcaaccagcg cttccagcgc aagccggtgg tgctgcccgt
     4861 gatcctggag gtgtgatggc cgagaaggtg acgatcgaaa aggtactgcc ggaccggacc
     4921 cccggtccaa acggccacgg cccccgcgag tcgcgcgagg cgtcgcgtcc cggggcgctc
     4981 aagcggagcg ggccgcggaa gagcaagggc gagcccggtc gctgggtgcg ggaggccaag
     5041 ggcatcctgg ccctggccct cgccggcttc ggcttcgtgg cgctctacgc ctatgacccg
     5101 acgctgcatc cgctggacca gtcgagcccg gtggggccgg tcggcgggtg gctcggctgg
     5161 gggtcgttcc acctgttcgg ctacgcggga tatctcttcc cggtgctgtt cacgctctac
     5221 ggcgtctccg ccttcgtgcg cccgcgcatg gcgcacggct ggcaggcggg cgccggcctc
     5281 gcgatcctcc tggtgagcgc gaccggtatc ctcgcccgcg cctcggacac gctggccgag
     5341 ttccgcgtgc acaagggcgg ggtggtcggc tgggcggtgt ccgaggccct ccggctctcg
     5401 atcggcacgg tggggacgtg gatcatcctg ctggccctga tcccgctggg cgtgctgttc
     5461 gtcacgcgca ttccctacag cgtgctctcg cgcggtctgc gggcccgctt cggccggctg
     5521 cggcgggcgg aagccaagaa ggggcggagc cagtcccccg cgtccccggg cgcgcccgtg
     5581 atcgcggcgg cgctcgcggg ccttcccgcc atcgaggccg acgtgccgcc gcggctcgtg
     5641 gtgaaggagc cgcccaaggc caagtcgagc ctctccgaca agggcctcgc gtggcaggag
     5701 acgttcgact tcggcaaggg cggcgcggag tcgttcaagc tgcccgcggt cggcctgctc
     5761 gccccgccgc ccgcctcctc gctccagcgc acccgcgagg agctcgagac caacgcgcag
     5821 acgctcaaga agaagctcca cgacttcggg gtcgacggcc acatcgtgca ggcgagcccg
     5881 gggccggtga tcacctccta cgagttcgag cccgcgtccg gggtgaaagt gagccaggtg
     5941 gtgaacctgg ccgacgacct ggccctggcc atgaaggtgg cggcggtccg catcatcggg
     6001 ccgatcccgg gccgcggcac ggtggcggtg gaggtgccga accccgagac cacgacggtc
     6061 tacctccgcg agatcctggt gagccaggag ttcgtcacga acaaggcgcg gctgccgctc
     6121 gccctcggca aggacaccac cggcaactcg gtgatcgcgg acctggccgc gatgccgcat
     6181 ctgctcatcg cgggctcgac gggcagcggc aagtcggtcg ggctcaacac catgatctgc
     6241 tcgctgctct acaaggcgac gcccgccgac gtgcggttcc tcctgatcga tccgaagcgg
     6301 ctcgagctcg gggtctacga gaagatcccg cacctgctcg ccccggtggt gaccgacgcc
     6361 aaggaggcct ccgcgcgcct caagtggatc gtgggcaaga tggacgcccg ctacaagcag
     6421 ctgcaggcca aggcggtgcg caacatcgag ggctacaaca aggaggtgcc gccggaggag
     6481 cggctgccct actgggtggt ggtggtcgac gagctggccg acctcatgat ggtctcggcc
     6541 ggcgaggtgc agaactcgct gatccgcctg gcccagatcg cccgcgcggt cggcatccac
     6601 ctgatcgtgg cgacccagcg gccgtctgtc gacgtggtga ccggcctcat caaggcgaac
     6661 ttcccgaccc gcatcgcctt ccaggtggcg tcgaaggtgg actcgcgcac cgtgctggac
     6721 gccaacgggg ccgagcagct gctgggccgc ggcgacatga tcttcgtccc gccggggcag
     6781 agccgccaga tgcgtgtgca cggctcgtgg gtctccgaca aggaagtgaa ggcgatctgt
     6841 gagttcctgc gcacccaggg ctcggcggtg tacgagcacg tggagctgcc gtccgaggag
     6901 gcgggctacg gcgggggcgg ggaagatcgc gacgacatct actgggacgc ggtcaacctg
     6961 gtgatcgccc agcgggccgc gtcgatctcg ttcctgcagc ggcggctcgg gctcggctac
     7021 cccaaggccg cgcggttcat cgacatgatg gagcaggacc acgtca
//
LOCUS       DATMCV010000835         5685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_178912, whole genome shotgun sequence.
ACCESSION   DATMCV010000835 DATMCV010000000
VERSION     DATMCV010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178912"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VNU03_19210"
     CDS             <1..850
                     /locus_tag="VNU03_19210"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HWP76659.1"
                     /translation="LLHEEMDVTFRVVRDLFSPEVEEFLVDSEAAYEKCLHFATSLVP
                     QLAPRVKRWDKDAPIFEATGIERDIDKALRRRVWLKSGGYIVIDHTEALVAIDVNTGK
                     YVGKRDLEETILKINLEAVTEVVRQIRLRDLGGIIIIDFIDMESFEHRDQVFKALRRV
                     LADDKARTNVLEISELGLVEMTRKRVRQSLQSLFCAPCPTCKGSGVVKSDATLAAEIF
                     RKIQAGAHDGGPREVVVRVHPEMAHHLESTQHDGLERLQALIGRKVVVQGASSYHREQ
                     YELTFK"
     gene            855..1211
                     /locus_tag="VNU03_19215"
     CDS             855..1211
                     /locus_tag="VNU03_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76660.1"
                     /translation="MTRPSRISRAAIVAQLRAAATKGDRSALALAIDQMKTWAYSPRY
                     WQKYLQLLTHPMARLVDLTVIKQGDKIAHQKGWVKPKREPRKPKRAVEPRPAGRARRG
                     ARSTPAATQPSLFPES"
     gene            1301..2068
                     /locus_tag="VNU03_19220"
     CDS             1301..2068
                     /locus_tag="VNU03_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76661.1"
                     /translation="MGRTGAIGLIGGLAVLAGTGAFLIPPIPQDPAYHRLADARSWLG
                     IPNALNVLSNAGFVLVGALGLGFLGDGGATARAFREPGERWPYTVFFAGLLLTGLGSA
                     YYHWAPGNPRLAWDRLPLAITLMGLLDAVIAERVGVRAALRLLGPLVAVAALSVGYWA
                     LTEQRGAGDLRPYALVQFYPLLAIPLMIWLLPPRYTGSAGLLAAAGIYALAKLPELAD
                     AWILSATRVVSGHTLKHVLAAVAGYSVLRMLRDRQAV"
     gene            2081..2362
                     /locus_tag="VNU03_19225"
     CDS             2081..2362
                     /locus_tag="VNU03_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWP76662.1"
                     /translation="MMDDQTKGKALGRLRRIEGQVQGIQRMVEEDKYCVDILLQLTAV
                     QGAVEQVQKLLLGQHLETCVADAVRSGSSRDRQRKLDELLDVFSRFSGR"
     gene            2374..4638
                     /locus_tag="VNU03_19230"
     CDS             2374..4638
                     /locus_tag="VNU03_19230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWP76663.1"
                     /translation="MSPKTSDAPRLDLPVKGMHCAACVGKVERALRSVPGVTDAAVNL
                     ATERARVELGPEPAALDQLRAAVAAAGYTIPEQIAVTPESRERDQADRAREIARLRVK
                     LVVGALLAVPVLLGSMTELFPWAPGWLRDPRLLWLLTTPVQFWVGGEFHAAFLRELRH
                     RTTSMNTLVSIGTNAAYFFSVAVTLWPHAFMAAGAMPYFEASALLMTFLVLGRWLEAR
                     ARGGTSEAIRRLVALQPRTARLAEPGGERDVPIAEIVPGDLLRVRPGERIAVDGLVVE
                     GASSVDESMLTGESLPVERGPGAPVVGGSINRTGTFTFRATRVGRDTVLARIVRLVEE
                     AQGSKAPIQRLADRVASVFVPVVLGIAALTFGAWMLWGPAPAFFHALTNAVGVLVIAC
                     PCALGLATPTAVMVATGRGAELGVLIRSAEVLETLHRTHTVVMDKTGTLTMGRPAVTS
                     VVPAPGIETDLVLALAAAVEQGSEHPLGEAIVTAAKSRGLALPPVREFRAVPGQGVEA
                     LVEQGRVLLGNQRLMDGRGVEPGSLAEPARRLAQGGQSVVYVAVAGAPLGLIAVADVL
                     RPEAPAAVAALRGMGVDVIMLSGDAQSTAAAIARQAGIERVHADVLPEQKATEIKRLQ
                     ADGRLVAMVGDGINDAPALAQADVGLAMSSGTDVAIEAADVTLMRDDLRGVVAAIELS
                     RRTMRIVKENLGWAFGYNLVLVPVAAGMLYPIWGIQLSPILAGLAMALSSVSVVTNSL
                     RLGRFRPSAWGTER"
     gene            4641..4787
                     /locus_tag="VNU03_19235"
     CDS             4641..4787
                     /locus_tag="VNU03_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWP76664.1"
                     /translation="MAKDPVCDMDVDPKSAAAQSKYQGQTYYFCAVGCKVKFDADPPK
                     YLAR"
     gene            4784..5680
                     /locus_tag="VNU03_19240"
     CDS             4784..5680
                     /locus_tag="VNU03_19240"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HWP76665.1"
                     /translation="MIGSPFLAGRDRYERVMDGRVDNTHDAAFTHTVRLSDPDRSVEV
                     TAVCTPSPGYEVREASVRVLSGAVDPGIARDFPGLAGARMVGGFTKRLADLCGPREGA
                     GLLVDAGIEVARLSRQVTKMPAAAVASLAPGDSQACWALDMAGWVDLPDSCFTYSPAG
                     RALFGTRAVSSPMVPALYCPPPGMLGVFTRKKVARLVVTGSRLHLFHSMHDNVHGFDV
                     HYEVDLDRGTIVAADSITSRLPYAGICSEPQSRVESLRGQAVDVALRKRIQGLLGGIG
                     GCAQLYDLTSDLLKLLSIESNS"
BASE COUNT          769 a         2018 c         2063 g          835 t
ORIGIN      
        1 gctcctccac gaggagatgg acgtcacctt ccgggtggtg cgggatctct tctcgccgga
       61 ggtggaggag ttcctggtcg acagcgaggc cgcctacgag aagtgcctgc acttcgcgac
      121 ctcgctggtg ccccagctcg ccccgcgggt caagcggtgg gacaaggacg ccccgatctt
      181 cgaggccacc ggcatcgagc gggacatcga caaggccctg cgccgccggg tgtggctcaa
      241 gtcgggcggc tacatcgtga tcgaccacac cgaggccctg gtggcgatcg acgtcaacac
      301 cggcaagtac gtgggcaagc gcgatctgga ggagaccatc ctcaagatca acctggaggc
      361 ggtgaccgag gtggtccgcc agatccgcct gcgggacctc ggcggcatca tcatcatcga
      421 cttcatcgac atggagagct tcgagcaccg cgaccaggtg ttcaaggccc tgaggcgggt
      481 gctcgcggac gacaaggcgc gcaccaacgt gctggagatc tcggagctgg gcctggtgga
      541 gatgacgcgc aagcgcgtgc gccagagcct ccagtccctc ttctgcgcgc cgtgccccac
      601 ctgcaagggc agcggggtcg tgaaatcgga cgccaccctc gcggccgaga tcttccgcaa
      661 gatccaggcg ggggcgcacg acggcgggcc ccgcgaggtg gtcgtgcggg tccaccccga
      721 gatggcgcac cacctcgagt cgacccagca cgacggcctc gagcgcctgc aggcgctgat
      781 cgggcgcaag gtcgtggtgc agggcgcgtc ctcctatcac cgcgagcagt acgagctgac
      841 gttcaagtga ccccgtgacc cgcccgtcgc gcatcagccg ggccgccatc gtcgcccagc
      901 tccgggcggc cgcgaccaag ggagatcgct cggccctggc cctcgcgatc gaccagatga
      961 agacctgggc gtactcgccc cgctactggc agaagtacct ccagctgctg acccacccga
     1021 tggcgcgcct ggtggacctg accgtcatca agcagggcga caagatcgcc caccagaagg
     1081 gctgggtgaa gcccaagcgc gagccgcgca agccgaagcg ggcggtcgag ccgcgcccgg
     1141 cgggccgggc gcggcggggc gccaggtcga cgcccgcggc aacgcagcct tccctctttc
     1201 ccgagagctg atcgccccac cgtcccgccg gtggctccgc cggtggctcc gccttcgacc
     1261 actggatgac tacccccgta gggtatatgc tgctctcgac atggggcgca cgggggcgat
     1321 cgggctgatc ggcgggctgg cggtgctggc ggggacgggg gcgttcctga tcccgccgat
     1381 cccgcaggac cccgcctacc atcggctggc cgacgcgcgg agctggctcg gcatccccaa
     1441 cgcgctgaac gtgctctcca acgcgggctt cgtcctggtc ggcgccctcg gactgggctt
     1501 tctcggcgac ggcggggcca cggcccgggc cttccgcgag cccggagagc ggtggcccta
     1561 caccgtcttc ttcgccggcc tgctcctgac cgggctcggc tccgcctact accactgggc
     1621 gcccggcaac ccgcggctcg cctgggatcg gctgccgctg gccatcaccc tcatgggcct
     1681 cctcgacgcg gtgatcgccg agcgcgtcgg ggtgcgggcg gctctgcgcc tcctgggccc
     1741 gctggtggcg gtcgcggccc tctcggtcgg ctactgggcg ctgaccgagc agcggggcgc
     1801 gggcgacctg cgcccgtacg cgctcgtgca gttctatccg ctgctcgcca tcccgctcat
     1861 gatctggctg ctgccgccgc ggtacaccgg gagcgccggg ctgctggccg ccgcgggcat
     1921 ctacgcgctg gcgaagctgc ccgagctcgc ggacgcgtgg atcctctcgg cgacccgcgt
     1981 ggtcagcgga cacaccctca agcacgtgct ggccgcggtg gccggctact cggtgctccg
     2041 gatgctccgg gatcgacagg cggtctgaaa cggagacacc atgatggacg atcagacgaa
     2101 ggggaaggcg ctcggtcgcc tgcgccgcat cgagggccag gtccagggca tccagcggat
     2161 ggtggaggag gacaagtact gcgtggacat cctgctccag ctcaccgcgg tccagggggc
     2221 ggtcgagcag gtccagaagc tcctgctcgg ccagcacctc gagacgtgcg tggcggacgc
     2281 ggtgcgctcc ggcagctcgc gggatcgcca gcggaagctc gacgagctgc tcgacgtctt
     2341 ctcccggttc tccgggcgat agggaggccc agggtgagcc cgaagacgtc cgacgccccg
     2401 cgcctcgatc tcccggtgaa gggcatgcac tgcgcggcct gcgtcggcaa ggtcgagcgg
     2461 gcgctccggt ccgtgccggg ggtgaccgac gccgcggtca acctcgccac cgagcgcgcg
     2521 cgcgtggagc tgggcccgga gccggccgcg ctcgaccagc tccgggccgc ggtcgccgcc
     2581 gccgggtaca cgattcccga gcagatcgcg gtcacccccg agagccgcga gcgagaccag
     2641 gcggaccggg ctcgcgagat cgcgcggctg cgcgtgaagc tcgtggtggg cgcgctcctc
     2701 gcggtgccgg tgctgctcgg cagcatgacc gagctcttcc cgtgggcgcc cggatggctc
     2761 cgggaccccc ggctcctgtg gctcctgacc acgccggtgc agttctgggt cggcggggaa
     2821 ttccacgccg cgttcctgcg cgagctgcgg caccgcacta cgagcatgaa caccctcgtg
     2881 tcgatcggca ccaacgcggc ctacttcttc agtgtggcgg tgaccctctg gccgcacgcg
     2941 ttcatggccg cgggggcgat gccctacttc gaggcctccg cgctcctcat gaccttcctg
     3001 gtgctcgggc gctggctcga ggcgcgcgcc cgcggcggca cctcggaggc gatccgacgg
     3061 ctcgtcgcgc tccagccccg caccgcgcgc ctggccgagc cgggaggcga gcgcgacgtg
     3121 ccgatcgccg agatcgtgcc cggcgacctg ctgcgcgtgc gtccgggcga gcggatcgcg
     3181 gtggacggcc tggtcgtcga gggcgcgtcc tccgtcgacg agtcgatgct gaccggcgag
     3241 agcctcccgg tcgagagagg ccccggggcc ccggtggtcg gcggctccat caaccgcacc
     3301 gggacgttca ccttccgggc cacccgggtg gggcgcgaca ccgtgctcgc ccggatcgtg
     3361 cggctggtcg aggaggccca gggctcgaag gcgccgatcc agcggctcgc cgaccgggtc
     3421 gcgtccgtct tcgtcccggt ggtgctgggg atcgccgcgc tcacgttcgg ggcctggatg
     3481 ctctggggtc ccgcgccggc cttcttccac gcgctcacca acgcggtggg cgtgctcgtc
     3541 atcgcctgcc cgtgcgctct cgggctcgcg acgcccaccg cggtgatggt ggcgaccggc
     3601 cggggcgccg agctcggggt gctgatccgg agcgcggagg tcctcgagac gctgcaccgc
     3661 acgcacaccg tggtcatgga caagaccggc acgctcacga tgggacggcc cgccgtcacc
     3721 tccgtggtcc cggcccccgg aatcgagacg gacctggtgc tggccctggc cgccgcggtc
     3781 gagcagggct cggagcatcc cctcggcgag gcgatcgtga ccgcggccaa gtcccggggc
     3841 ctcgcgctgc cgccggtgcg cgagttccgg gccgtgccgg ggcagggggt ggaagcgctg
     3901 gtcgagcagg gtagggtcct gctgggcaat cagcggctca tggacggtcg cggggtcgag
     3961 ccgggatctc tggcggagcc ggcgcggcgg ctcgcccagg gaggacagag cgtcgtctac
     4021 gtggcggtcg cgggcgcgcc gctcgggctc atcgcggtgg ccgacgtgct gcggccggag
     4081 gcgcccgcgg cggtggccgc gctccgcgga atgggcgtgg acgtgatcat gctgagcggc
     4141 gacgcgcaga gcactgcggc ggccatcgcg cgccaggccg gcatcgagcg ggtgcacgcg
     4201 gatgtgcttc cggagcagaa ggcgaccgag atcaagcgcc tgcaggccga cggccggctg
     4261 gtggccatgg tcggcgatgg gatcaacgac gcgcctgcgc tcgcccaggc cgacgtgggc
     4321 ctcgcgatga gctcgggcac cgacgtggcc atcgaggcgg ccgacgtcac cctcatgcgc
     4381 gacgacctgc ggggggtggt cgcggccatc gagctgtccc gccgcacgat gcgcatcgtc
     4441 aaggagaacc tggggtgggc gttcggctac aacctcgtgc tggtgccggt ggccgcgggt
     4501 atgctctacc cgatctgggg aatccagctc tcgccgatcc tggccgggct cgcgatggcc
     4561 ctctcgtcgg tatcggtggt caccaacagc ctgcggctcg gccggttccg tccgagcgcg
     4621 tggggaacgg agcgatgacg atggccaagg acccggtgtg cgacatggac gtggacccga
     4681 agagcgcggc cgcgcagtcg aagtaccagg ggcagaccta ctacttctgc gcggtgggct
     4741 gcaaggtgaa gttcgacgcc gatcccccca agtacctcgc ccgatgatcg gctccccgtt
     4801 tctcgccggc cgcgaccgct atgagcgggt gatggacggc cgggtcgaca acacccacga
     4861 cgcggccttc acccacaccg tgcggctgtc cgatccggat cggtccgtcg aggtgaccgc
     4921 ggtctgtact ccctcgccgg gatacgaggt gcgggaggcg agcgtgcgcg tgctgtcggg
     4981 cgcggtcgac cccggcattg cccgcgactt ccccggcctc gccggagcac ggatggtggg
     5041 cggattcacc aagcgcctgg ccgacctctg cggcccgcgc gagggggcgg ggctgctcgt
     5101 cgacgcgggc atcgaggtgg cccggctcag ccgccaggtg accaagatgc cggccgcggc
     5161 cgtggcctcg ctcgcgccgg gggattcgca agcatgctgg gcgctcgaca tggccgggtg
     5221 ggtggacctg cccgactcct gcttcaccta cagtcccgcc gggcgtgcgc tcttcggcac
     5281 gcgggcggtc tcctccccca tggtgcccgc gctctattgc cccccgccgg ggatgctcgg
     5341 cgtcttcacc cgcaagaagg tcgcccgtct ggtagtgacc gggtcccggc ttcacctgtt
     5401 ccactccatg cacgacaacg tgcacggctt cgacgtgcac tacgaggtcg acctcgaccg
     5461 gggcaccatc gtggcggccg actccatcac gtcgcggctg ccgtatgccg ggatctgctc
     5521 cgagccgcaa tctcgcgtcg agtccctgcg cgggcaggcg gtcgacgtcg ctctccggaa
     5581 gcgcatccag ggcctcctcg ggggcatcgg gggctgtgcc cagctctacg acctcacatc
     5641 cgacctgctc aagctcctga gcatcgagtc aaatagctga taaat
//
LOCUS       DATMCV010000836         8424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_777030, whole genome shotgun sequence.
ACCESSION   DATMCV010000836 DATMCV010000000
VERSION     DATMCV010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777030"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VNU03_19245"
     CDS             complement(<1..476)
                     /locus_tag="VNU03_19245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWP76666.1"
                     /translation="MEMRELTIRKRDGIGKGPAKRLRRSGQVPATLYGGAAPVNIAVT
                     PKDVLRLIHGHEGSTQLFRVTFEGGKDGKMVIIRDMQFDPVSESLIHVDLQEVAMDRA
                     IQVSVAIHHVGDSVGVKDASGILEMVLREVRVSCLPGNIPEFLEADVTNLNIHDVLT"
     gene            complement(504..1445)
                     /locus_tag="VNU03_19250"
     CDS             complement(504..1445)
                     /locus_tag="VNU03_19250"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWP76667.1"
                     /translation="MSYELKLFSGNANRPLAEEIAKTLQLPLGDADVSRFSDGEVYVQ
                     INENVRGQDIFVIQPTCPPVNDHLMELLVMIDAFKRASARRITAVLPYYGYGRQDRKV
                     QGRMPITAKLVADLLTAAGTQRLLAVDLHAGQIQGFFDIPVDHLFAAPPVIIDYLAKK
                     DLKDPVLVSPDAGGVERARALAKRLKAGLAIIDKRRDGNNVAVFMHLIGDVKDKDVVI
                     ADDMIDTAGTLIQAVDALRREGARRILACAVHGVLSGPAIARIKESVLEEVIITNSIP
                     LTPEHQLPKIRVLSVAPLLAEAIRRIHDEESVSTLFV"
     gene            complement(1571..1644)
                     /locus_tag="VNU03_19255"
     tRNA            complement(1571..1644)
                     /locus_tag="VNU03_19255"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1610..1612),aa:Gln,seq:ttg)
     gene            complement(1651..2514)
                     /gene="ispE"
                     /locus_tag="VNU03_19260"
     CDS             complement(1651..2514)
                     /gene="ispE"
                     /locus_tag="VNU03_19260"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWP76668.1"
                     /translation="MLRASAKVNLALEVLGKRGDGYHEIATVLQAVDLFDRLTVETAD
                     TLSLHADDPELPTDEGNLVMRAARLLQKTAGIEKGARIELQKRIPVAAGLGGGSSDAA
                     ATLWGLNRLWGLRWPRARLQELAVELGMDVPFFLGTGRAVARGRGERLQALPAGGGYA
                     LVLVNPRAPLSTREVYGRVPAGWRAEPTGTERVIEALRRRNVATLAAALTNNLERVVE
                     PVLPVIGRMKAALLAAGALGAIMSGSGPTVFGLARSLDHARQVRSRVSRAGWACWAVR
                     TNSGPAIRASR"
     gene            complement(2582..3367)
                     /locus_tag="VNU03_19265"
     CDS             complement(2582..3367)
                     /locus_tag="VNU03_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76669.1"
                     /translation="MRRPRPSRSSRLGALLLAAVLGGCATTAPAPAPPPVTEEARAAL
                     ARIEQHRLSLHDLRSRADITIRRSGRAQRLTGVLLLLGEPATMRFEALSPLGTPVLIV
                     AGDPKSVTLWEVLDNRAYIAPASPDANRRWLGLALGVEDLVALLGGRVRAMPDPTTVE
                     LVAADAVGPSLRLTGAPGEQRIWFDPDSGQARQVEWTGKNPARVTFTPAPADGPPAGF
                     TLETADGSLRVAIVYREPRMNTSFDPALLTLTVPQDVRIQDFR"
     gene            complement(3339..5558)
                     /locus_tag="VNU03_19270"
     CDS             complement(3339..5558)
                     /locus_tag="VNU03_19270"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HWP76670.1"
                     /translation="MGDVLLAALPALQERLLAERIRLGWRAAVGADLSRRSRPGELKA
                     GTLTIMVDNSPWLQELSMRSAEVLSAVRARFGPSVTALRLSLAGPAPAPERPARRATP
                     PTGSSRLDPDETALVESALAPVQDPDLATTVRRLMSKDLIARRPRGLAALALGGAALA
                     ATLAGCASGGTGPADPGQPAAAVDAAPMVRRPIGAQGEAYDHYVIAQMEARAGRLPQA
                     IAQLREAIKTDPDSGVLWIQLSQWLVRQNDVTGAFTAAQKAIALEPDNASAYMTLADL
                     YKRQRRMPEAEAALEKAVALDPKTPDGYLALAQLHFEQKNYDKARAVLGRLVAARPNL
                     SQGYYLLGRIGIENEQWDDALTNLKRAVELDPDHDGAWSALGFVYEAQNKLDDAVAVY
                     KEALKANPDNAAFVERLGDLLVKLGRYGEAQNEIEALVESLPRDPRVWLKLGAIHYEQ
                     KQYDRAVSAFRQAVTLEPGNMRTRYYLATALMDAGKDDEARAELEKILQADPRSVDAR
                     VQLGFLYGRAKRYDDAVSTLQEAVNLEPKRPELFLYLGTALQRANQYDRAANALREGL
                     SVDDKSKDLHFQLGVVQEKQQRFDDAIRSFRRVLAIDSKHAESYNYIGYMYAERGTNL
                     GEAIQLIQQALALEPENGYFIDSLGWAYYQQGRYPEALRELKRAVSLAKEDPVLYEHL
                     GDAYLKNNLSNEAIEAWEKSLKTDPDSPTAPQVRTKIKELRESQTRAKGAPPKAEQK"
     gene            5720..7147
                     /gene="rlmD"
                     /locus_tag="VNU03_19275"
     CDS             5720..7147
                     /gene="rlmD"
                     /locus_tag="VNU03_19275"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HWP76671.1"
                     /translation="MARPKRGEILSLTIDDLAFGGEGVGRADGYVVFVPGALPGDRLQ
                     VRLVQVRSRFGRGVIEAVLEPSPQRVEAPCPYFGRCGGCRLQHVAYEAQLTFKSKQVA
                     DALERLGGLRDVPVRPIIGADEIFGYRNKMEFTVARARRPAGSEPAGSGPPRHEPPRH
                     EIVVGLHETERYDSVLDIERCLLQSDRMNGLLGEARAFFAERGLTVYEQDTGEGLLRF
                     LMLREGRQTGELMTNVVTSAPAVSELAPLVERLQARDAGTTSVVMNVNPKKASVAVGV
                     EEHLLGGRDHIRESVGGLTFRVSANSFFQTNTRQAERLFDLVVESTGLTGTETVLDLY
                     SGTGAISLLLARRARWVYGVELAQAAVDDAGANAAANSITNCTFLSGEVRFVLPELIA
                     KGVTAEVVVADPPRAGFHPKALHALITLGARRIVYVSCNPTTLARDLGELVRGGYRLE
                     WVQPVDMFPHTPHIEAVARLERVTS"
     gene            7144..8061
                     /gene="nikB"
                     /locus_tag="VNU03_19280"
     CDS             7144..8061
                     /gene="nikB"
                     /locus_tag="VNU03_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HWP76672.1"
                     /translation="MRSYLRRRLWQSLLVLLGVSVVVFLILHLTGDPAALLLPPDATA
                     EDIAKFRTAMGFDDPVAVQYVRFLKGAVRGDFGESLRHGEPAMALVLERLPATFELAG
                     AGLLIALGLAIPAGIISAVRRNTSVDYVSTVVALLGQAMPTFWLGIMLILIFSVRLNW
                     LPSSGRGGLEHLILPAITLGLFTTARITRLTRSGMLEVLGQDYIRTARAKGVGEPPVV
                     WKHALKNASIPIVTIVGIELGTLLGGSVITETIFAWPGVGRLSVQAIFNRDYPVVQSA
                     VFLLASTFVIVNFLVDVIYTYLDPRIRFR"
     gene            8142..>8424
                     /locus_tag="VNU03_19285"
     CDS             8142..>8424
                     /locus_tag="VNU03_19285"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76673.1"
                     /translation="MTLLRRLVRRRTALFGMLVVLGVLLAAAFAPLVAPFDPLEQDIG
                     QRLREPGWQDAQGRVHPLGTDHLGRDILSRIVFGSRIALVVGLAAVLISG"
BASE COUNT         1122 a         2930 c         3020 g         1352 t
ORIGIN      
        1 gtgagcacgt cgtggatgtt caggttggtg acgtcggcct ccaggaactc ggggatgttg
       61 ccgggcaggc aggagacccg cacctcgcgc agcaccatct cgaggatgcc ggaggcgtcc
      121 ttgaccccga ccgaatcgcc cacgtggtgg atggccaccg agacctggat ggcccggtcc
      181 atcgccacct cctgcaggtc gacgtggatg aggctctccg agaccggatc gaactgcatg
      241 tcccggatga tcaccatctt gccgtccttg ccgccctcga aggtcacccg gaacagctgg
      301 gtgctgccct cgtggccgtg gatcagccgg aggacgtcct tgggcgtcac cgcgatgttc
      361 accggggccg cgcccccgta gagggtggcc gggacctgcc cgctgcggcg cagccgcttg
      421 gccggaccct tgccgatgcc gtcgcgcttc ctgatggtca gctcacgcat ttccatgatt
      481 cgttgctcct tgagtgggtg gtcttacacg aagagggtcg acaccgactc ctcgtcgtgg
      541 atgcgacgga tcgcctccgc gagcaggggg gcgaccgaga gaacgcggat cttgggcagc
      601 tggtgctcgg gagtgagcgg gatcgagttc gtgatgatga cttcttccag gacggactcc
      661 ttgatgcggg cgatggcggg tccggacagc actccgtgca ccgcgcaggc gaggatgcgc
      721 cgggcgccct cgcgccggag cgcgtcgacc gcctggatca aggtgcccgc ggtatcgatc
      781 atgtcgtcgg cgatcacgac gtccttgtcc ttgacgtcgc cgatgaggtg catgaacacc
      841 gccacgttgt tgccatcgcg ccgcttgtcg atgatggcga ggccggcctt gagccgcttc
      901 gcgagggccc gggcgcgttc cacgcctccc gcgtcggggg agaccaggac ggggtccttc
      961 agatccttct tcgccaggta gtcgatgatc accggcgggg cggcgaagag gtgatccacc
     1021 gggatgtcga agaagccctg gatctggcct gcgtggaggt ccaccgcgag cagtcgctgg
     1081 gtgccggccg cggtgaggag gtcagccacg agtttcgcgg tgatcggcat tcgtccctgc
     1141 accttgcggt cctggcggcc gtagccgtag tagggcagga ccgcggtgat ccgccgcgcg
     1201 gaggcgcgct tgaaggcgtc gatcatcacg agcagctcca tcagatgatc gttgaccggc
     1261 ggacacgtgg gctggatcac gaagatgtcc tgcccccgga cattctcgtt gatctgcacg
     1321 tacacctcgc cgtccgagaa gcgggagacg tccgcgtcgc ccagcgggag ctggagcgtc
     1381 ttggcgatct cctcggccag gggccggttc gcgttgcccg agaacagctt cagctcgtag
     1441 gacatggttt ctcccgtgat gccatcgcgg ctgtccggat tgcggctgct acccttgctt
     1501 ccctttcaaa tgcgctcggc tcgccttcgg ctgcacctcg ccaagccact gcacctcgcg
     1561 acgcgcgagg tggctggggc gggaggaatc gaacctccat tgcggggtcc aaagccccga
     1621 gtcctgccac tgaacgacgc cccagtgccg tcatcggctc gcccggatcg ccggtcccga
     1681 gttcgtcctg accgcccagc acgcccagcc ggcccggctc acccgcgatc gcacctgccg
     1741 cgcgtggtcg agggaccgcg cgaggccgaa caccgtcggg ccgctgcccg acatgatcgc
     1801 cccgagggct cccgcggcca agagggcggc cttcatgcgg ccgatcacgg gcagcaccgg
     1861 ctcgaccacc cgctccaggt tgttcgtcaa cgccgccgcg agggtggcca cgttgcgccg
     1921 ccgcagtgcc tcgatcaccc gctcggtgcc cgtgggctcc gcgcgccagc ccgccggcac
     1981 gcgaccgtag acctcgcggg tggagagcgg cgcgcgcgga ttgaccagca ccaacgcgta
     2041 ccccccgccg gccggcagcg cctgcagccg ctcgccgcgc cctctcgcca ccgcccggcc
     2101 ggtacccagg aagaacggca cgtccatgcc gagctccacc gccagctcct gcagccgggc
     2161 ccggggccac ctgagccccc acagccggtt gaggccccag agcgtggccg ccgcgtcgct
     2221 cgacccgccg cccagcccgg ccgcgaccgg gatccgcttc tgcagctcga tccgcgcgcc
     2281 cttctcgatc cccgcggtct tctgcagcag ccgcgccgcg cgcatcacca gattcccttc
     2341 gtcggtgggc agttcgggat catcggcgtg tagcgaaagg gtgtcggcgg tttccacggt
     2401 caggcgatcg aagaggtcca ccgcttgcag caccgtcgcg atctcatggt agccgtcgcc
     2461 ccgcttcccc agcacctcga gggccaagtt caccttggcc gaggcgcgga gcacgagcct
     2521 ctggcttccc cgcggccgct tcgacaaggc agcacacgct atcacgcgtc gagacggagt
     2581 gtcaacggaa gtcttgaatt ctaacgtctt gcggaacagt gagggtcagc agggcggggt
     2641 cgaacgacgt gttcatgcga ggctcacggt acacgatcgc gacccgcagc gatccgtccg
     2701 cggtctcgag cgtgaagccg gccggcgggc cgtcggccgg ggccggcgtg aacgtcacgc
     2761 gcgccgggtt cttgccggtc cactccacct gtcgggcctg gccgctgtcg ggatcgaacc
     2821 agatgcgctg ctcgcccggc gcgccggtga gccggaggga cgggccgact gcgtcggcgg
     2881 ccacgagctc gaccgtcgtc ggatcgggca tcgcccgcac ccgaccgccg agcagggcca
     2941 ccaggtcctc gaccccgagc gccaggccca gccagcgacg gttggcgtcc ggggaggccg
     3001 gggcgatgta ggcccggttg tcgagcacct cccagagggt gacgctcttc ggatcgccgg
     3061 ccacgatgag caccggcgta ccgagaggag agagagcctc gaagcgcatg gtggcgggct
     3121 cgcccaggag cagcagcacc ccggtgaggc gctgggcccg tccgctgcgg cggatggtga
     3181 tgtcggcccg cgagcgcagg tcgtgcagcg agagacggtg ctgctcgatc cgcgccagcg
     3241 cggcgcgcgc ctcctcggtg accggcgggg gcgcgggggc cggcgcggtg gtcgcgcagc
     3301 cgcccagcac cgccgccagc aggagggcgc cgaggcggct acttctgctc ggccttgggc
     3361 ggcgcaccct tggcccgggt ctggctctcc cgcaactcct tgatcttggt gcggacctgg
     3421 ggcgcggtgg ggctgtccgg atccgtcttc agcgacttct cccacgcctc gatcgcctcg
     3481 ttggagaggt tgttcttgag gtaggcgtcg cccaggtgct cgtagaggac ggggtcttcc
     3541 ttggcgaggc tcaccgcgcg cttcagctcg cggagggcct ccggatagcg gccctgctga
     3601 tagtaggccc agcccaggct gtcgatgaag tagccgttct cgggctcgag ggccagcgcc
     3661 tgctggatga gctggatcgc ctcgccgaga ttcgtgccgc gctcggcgta catgtagccg
     3721 atgtagttgt acgactcggc gtgcttggag tcgatcgcga gcacgcgacg gaaggagcgg
     3781 atcgcgtcgt cgaaccgctg ctgcttctcc tgcacgaccc cgagctggaa gtggagatcc
     3841 ttgctcttgt cgtcgaccga cagcccctcg cgcagcgcgt tggcggcccg atcgtactgg
     3901 tttgcgcgct gcagcgcggt gccgaggtag aggaacagct cgggccgctt cggctccagg
     3961 ttgaccgcct cctgcagggt ggacaccgcg tcgtcgtagc gcttggcgcg cccgtagagg
     4021 aagccgagct gcacgcgcgc gtcgaccgag cgggggtcgg cctggaggat cttctcgagc
     4081 tcggcgcggg cctcgtcgtc cttgcccgcg tccatcagcg cggtggcgag gtagtagcgg
     4141 gtccgcatgt tgcccggctc gagggtcacc gcctgccgga acgcggacac cgcccggtcg
     4201 tactgcttct gctcgtagtg gatggcgccc agcttgagcc acacccgcgg atcgcgcggc
     4261 agggactcca cgagggcctc gatctcgttc tgggcctcgc cgtagcggcc gagcttgacg
     4321 agcaggtccc cgaggcgctc cacgaacgcg gcgttgtccg ggttggcctt cagcgcttcc
     4381 ttgtagaccg cgaccgcgtc gtccagcttg ttctgcgcct cgtagacgaa gccgagggcc
     4441 gaccacgcgc cgtcgtggtc cgggtcgagc tcgaccgcgc gcttcaagtt ggtcagcgcg
     4501 tcgtcccact gctcgttctc gatgccgatg cggccgagca ggtagtagcc ctgcgagagg
     4561 ttggggcgcg cggccaccag ccgccccagc accgcgcggg ccttgtcgta gttcttctgc
     4621 tcgaagtgca gctgggccag ggccaggtag ccgtccgggg tcttgggatc gagcgcgacc
     4681 gccttctcga gcgcggcctc cgcctccggc atccggcgct gccgcttgta gagatcggcg
     4741 agcgtcatgt acgcgctcgc attgtccggc tcgagcgcga tcgccttctg cgccgcggtg
     4801 aacgcgccgg tgacgtcgtt ctggcggacc agccattgcg agagctggat ccagaggacc
     4861 ccggagtcgg gatcggtctt gatcgcctcg cgcagctggg cgatggcctg gggcaggcgg
     4921 ccggcccggg cctccatctg ggcgatcacg tagtggtcgt acgcctcgcc ctgcgcgccg
     4981 atgggccggc ggaccatcgg cgccgcgtcg accgccgcgg ccggctggcc ggggtcggcc
     5041 ggacccgttc cgccgctggc gcagccggcc agcgtggcgg cgagggccgc cccgccgagg
     5101 gccagagccg cgagcccgcg ggggcggcgt gcgatcaggt ccttcgacat gagccggcgc
     5161 accgtcgtgg ccagatcggg gtcctgaacg ggggcgagcg cgctctccac gagcgcggtc
     5221 tcgtcgggat cgagacggga ggaaccggtg gggggcgtcg cgcgccgcgc gggccgctcc
     5281 ggcgcgggcg ccgggccggc gaggctcagg cgcagcgcgg tgacggacgg gccgaagcgg
     5341 gcacggaccg cgctgaggac ttccgcggat cgcatggaga gctcctggag ccagggtgag
     5401 ttgtcgacca tgatcgtgag cgtacccgcc ttcagctcgc cgggtcgcga gcggcgcgag
     5461 agatcggcgc ccaccgccgc tcgccagccg agacggatgc gctcggcgag cagccgttcc
     5521 tggagagccg ggagggcggc gagcagcacg tcgcccacgc ggacggggga gggagagcga
     5581 ttggcactcc gcatcgcttt cccatgatac gcgaccggct atctcatcgc tacttcagac
     5641 ctcatcgtcc gcgatcatgg cgcgcggggg gtggggtcaa attgactccg atccgacgga
     5701 cctctatagt gtcgctacca tggcgcgacc caaacgcggg gagattctgt ccctgacgat
     5761 cgacgacctg gccttcggcg gcgagggcgt cgggcgggcc gacggatacg tggtcttcgt
     5821 gccgggcgcg ctgccgggtg atcggctgca ggtgcggctc gtccaggtgc gctcccggtt
     5881 cggtcgcggt gtcatcgagg cggtgctcga gccgtcgccc cagcgggtgg aggcgccgtg
     5941 cccgtatttc gggcggtgcg gggggtgccg gctgcagcac gtggcctatg aagcccagct
     6001 caccttcaag tccaagcagg tggcggacgc gctcgagcgc ctgggcggcc tgcgggacgt
     6061 ccccgtccgc ccgatcatcg gcgcggacga gatcttcggc taccggaaca agatggagtt
     6121 cacggtggcg cgcgcccgcc ggcccgcggg gagcgagccg gcggggagcg ggcccccgcg
     6181 gcatgaaccc ccgcggcatg aaatagtcgt cggcctgcac gagaccgagc gctacgactc
     6241 ggtgctcgac atcgagcgct gcctgctcca gtcggaccgg atgaacggtc tcctcgggga
     6301 ggcgcgcgcc ttcttcgccg agcgcgggct caccgtgtac gagcaggata ccggcgaggg
     6361 gctgctgcgc ttcctgatgc tgcgggaggg ccggcagacc ggcgagctga tgaccaacgt
     6421 ggtgacctcg gcgcccgcgg tgtccgagct ggccccgctg gtggagcgcc tgcaggcccg
     6481 cgacgcgggg accacgagcg tggtcatgaa cgtgaacccc aagaaggcga gcgtggcggt
     6541 cggggtggag gagcacctgc tgggcgggcg cgaccacatc cgcgagagcg tgggcggtct
     6601 caccttccgg gtctcggcga actccttctt ccagacgaac acccggcagg cggagcggct
     6661 cttcgacctc gtggtcgagt cgaccggcct gaccggcacc gagaccgtgc tcgacctgta
     6721 ctcgggcacc ggcgcgatca gcctgctgct ggcccgccgg gcgcgctggg tctacggggt
     6781 ggagctggcc caggccgcgg tggacgacgc gggggcgaac gcggccgcga actccatcac
     6841 caactgcacg ttcctgagcg gcgaggtgcg cttcgtgctg ccggagctca tcgccaaggg
     6901 ggtgaccgcg gaggtggtgg tggcggatcc gccgcgcgcg gggttccatc ccaaggcgct
     6961 ccacgccctg atcaccctgg gcgcccggcg catcgtctac gtctcgtgca acccgaccac
     7021 cctcgcccgc gacctgggcg agctggtgcg cggcggctac cggctggagt gggtccagcc
     7081 ggtggacatg ttcccgcaca ccccgcacat cgaggcggtg gcccggctgg agcgtgtgac
     7141 ctcgtgaggt cgtacctgcg gcggcgcctc tggcagtcgc tgctcgtgct cctgggcgtc
     7201 tcggtggtgg tgttcctgat cctgcacctg accggcgatc ccgccgcgct cctgctgccc
     7261 cccgacgcca ccgcggagga catcgcgaag ttccgcaccg cgatgggctt cgacgatccg
     7321 gtggccgtcc agtacgtgcg cttcctcaag ggcgcggtgc ggggcgactt cggggagtcg
     7381 ctgcgccacg gcgagcccgc gatggccctc gtgctcgagc gcctgcccgc caccttcgag
     7441 ctggccgggg cgggcctgct gatcgcgctc ggcctcgcga tcccggccgg catcatctcc
     7501 gcggtccgtc gcaacacctc ggtggactac gtctcgaccg tggtggccct gctcggccag
     7561 gccatgccaa ccttctggct cggcatcatg ctgatcctga tcttctcggt gcggctcaac
     7621 tggctgccct cctccggacg cggcggcctc gagcacctga tcctgcccgc gatcaccctc
     7681 gggcttttca ccaccgcgcg catcacccgg ctgacccgct cgggcatgct cgaggtgctc
     7741 ggccaggact acatccgcac cgcgcgggcc aagggggtgg gggagccgcc ggtggtctgg
     7801 aagcacgcgc tcaagaacgc gtcgatcccg atcgtcacca tcgtcggcat cgagctcggc
     7861 acgctgctcg gcggctcggt gatcaccgag accatcttcg cgtggccggg ggtgggccgc
     7921 ctctcggtcc aggcgatctt caaccgcgac tatccggtgg tgcagtcggc ggtgttcctg
     7981 ctcgcgagca cgttcgtgat cgtgaacttc ctggtcgacg tgatctacac ctacctcgat
     8041 ccgcgcatcc ggttccgctg atggccgcgc tgccgccgtc tgtcgctagt gaggccgtca
     8101 cgctcgcgac gccccctcgg agcgcggcgg aacgcgagtg ggtgaccctg ctccgtcgcc
     8161 tggtccgccg gcgcaccgcg ctcttcggga tgctcgtggt cctgggggtg ctgctggccg
     8221 ccgccttcgc cccgctggtg gcgcccttcg atccgctcga gcaggacatc ggccagcggc
     8281 tgcgcgagcc gggctggcag gacgcgcagg ggcgcgtgca tccgctcggc accgatcacc
     8341 tgggccgcga catcctctcg cgcatcgtct tcggctcccg catcgcgctc gtggtcgggc
     8401 tggccgcggt gctgatctcg gggg
//
LOCUS       DATMCV010000837         5972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_178932, whole genome shotgun sequence.
ACCESSION   DATMCV010000837 DATMCV010000000
VERSION     DATMCV010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178932"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VNU03_19290"
     CDS             <1..320
                     /locus_tag="VNU03_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76674.1"
                     /translation="LRLPQGAHDLILTHADGTLVFAQGSEDRQRHLSMHMTQVYRIAS
                     ATLARAGLGAMRRGVIEGSSEMTFLRSDGGVILGVALPREIEVGGGLVQVGRLWTDLN
                     IKA"
     gene            324..761
                     /locus_tag="VNU03_19295"
     CDS             324..761
                     /locus_tag="VNU03_19295"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HWP76675.1"
                     /translation="MADSQTSDSNAAHDPGTTVPRPSSRAQAFRETLFELIRLDGVRG
                     GLIVTPDGLVITSELPPRFPVEALAALAATLGRELELGASRLERSAFRTALFSADDGT
                     VFIGGSPVGYVTLVADRNVNMEAVRAALGRAVDRLRGTWRTAA"
     gene            complement(769..2862)
                     /gene="pilB"
                     /locus_tag="VNU03_19300"
     CDS             complement(769..2862)
                     /gene="pilB"
                     /locus_tag="VNU03_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HWP76676.1"
                     /translation="MAQIESKPLSPEEKRSLAESARLCEMIVEANPSDTGALETLKEI
                     YTKLGDRENLARVVARLAGTVGGRPSASVTPAPEPAAAPVPAAAAPLSPATIELDPGM
                     PGGDNGLGRGPGKRSATAVASERRQGSLSRLGDRLVAEKLITTEQLQRALAEQKGSAD
                     KLGTILVRLGFITEDSLVSFLSKQYSIPAITVAQVDPDPDVLKLVPEQIAKKHSVLPI
                     KRMGNVLTLAMADPTNVFALDDVGFMTGLQIQPVVASEAAIRKAFERLYETGGSVTDM
                     MSELEEADTDVEVVEGGEETFTKADVFDLKESADEAPVVRLINMILTDAIRRGASDIH
                     LEPYEKVFRVRFRIDGVLHEIMTPPKRLEAALTSRVKIMATLDIAERRLPQDGRIKLR
                     YHQREIDFRVSTLPTIFGEKTVMRILDKDALQLDLTALGFDPWSMEQFTKAIHSPYGM
                     ILITGPTGSGKTTTLYSAIHTINSPDINIMTAEDPVEYNLKGVNQVQINDEIGRTFAA
                     CLRSFLRQDPDVILVGETRDLETAQIGIRAALTGHLVLSTLHTNDCPSTPARLLDMGI
                     PPFLVSSSLQLILAQRLGRRVCRDCKEPYEASEESLVPYGHVPQGLGTVNFYKGKGCQ
                     TCNFTGMKGRVAIYEVMPVSTEIRDLILRNATTNEIQDAACSQGMKTLRQNALQKVID
                     GVTTVEEVLRVTLGT"
     gene            complement(2880..3368)
                     /locus_tag="VNU03_19305"
     CDS             complement(2880..3368)
                     /locus_tag="VNU03_19305"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HWP76677.1"
                     /translation="MSLNDNLAIHEEDAQRINGVLLHFLGESGAMEALLIDRSGQLLA
                     RGGASRSLDTVSLSALAAGAFSSTAAMARLLGETEFTMLFHQGVKESIHVAAVDEHAI
                     LLAIFDSRTTVGMVRLFAKEAGTAIGAILAETRTRPHRTGALAAPLHKDEVQSTLRER
                     PV"
     gene            complement(3355..3972)
                     /locus_tag="VNU03_19310"
     CDS             complement(3355..3972)
                     /locus_tag="VNU03_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HWP76678.1"
                     /translation="MSIINYAQKEISFKVVYYGTGVAGKTANLQYIYRGLPDVNRGNM
                     VSLATGDDRTLFFDFLPVSAVTVRNFVAKFQLYTVPGQVYYNMTRKLVLRGVDGIVFV
                     ADSQWDRLRENVESFRNLEENLKEYGYNLDEIPYVIQYNKRDLPNVAPVEYLEYLLNR
                     RGRRVPYFESVAVEGKGVFDTLNTVSRMVLVAGFGQEQGALNESK"
     gene            complement(3981..5246)
                     /locus_tag="VNU03_19315"
     CDS             complement(3981..5246)
                     /locus_tag="VNU03_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76679.1"
                     /translation="MAAPPLVVQAASPLVAEPPRLSDPVSVDLAPVVAPPIQEVVALP
                     PVVATSIPATEPPGVPEPELPIVSEPALRAEPPTAVASEVSRRIEIHPEPDATNIPAA
                     APLIVTPAPVLVAASPTGLHVGPSTTPEPRWKEPVPEPIVAKTAREWRVAPSVEPTPA
                     TPLADPAAAAEARRILTLLAPIASFEVTIQPMEGVTVFAMAAPAVAPETAVAATGLAL
                     PLLTDRRAPWPLDQITLRGPETALVLTPLSRGSSAASVLATAAPRGGSLALLEILCRR
                     VGAEGSRRVGSVPAPPDRTRNLVPAAVPDQVTRYASSFTAYGPVTASVLRDTEGEGVF
                     YFFLPRGADVPAVGAFAQDLQAVIRKAAGSGAVFRTAVLRSGNTLVVIQPEEVGHGRS
                     IVVVAGGDVTRPGLAYRQVERATAMLAHA"
     gene            5712..>5972
                     /locus_tag="VNU03_19320"
     CDS             5712..>5972
                     /locus_tag="VNU03_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76680.1"
                     /translation="MKPFRRKLGHHAAEGNADRGLVDLGDGHPSSHWLWLGNVDRPRQ
                     RSRLGDEPSRLLDPPRQLENGSRRRLDPRLGRELDDGPLRHDR"
BASE COUNT          907 a         2046 c         2066 g          953 t
ORIGIN      
        1 ggctccgtct accccagggc gcgcacgatc tgattctcac gcacgccgac ggaacgctcg
       61 tgttcgcaca ggggagcgag gaccgccagc ggcacctctc catgcacatg acccaggtct
      121 atcgcattgc ctccgccacc ctcgcccgag cgggccttgg cgcgatgcgc cgcggcgtca
      181 tcgagggctc atcggagatg acattcctga gaagcgacgg cggcgtgatc ctcggggtcg
      241 cgctccctcg cgagatcgag gtcggcggcg ggctcgtgca ggtcggccgg ctctggaccg
      301 atctcaatat caaggcctga gccgtggccg actcgcagac ctccgattcg aacgcggccc
      361 acgatcccgg gaccaccgtc ccgcgaccat cctcgcgggc ccaggccttc cgcgagacgc
      421 tcttcgagct gatccggctg gacggggtcc ggggcgggct catcgtgacc ccggacgggc
      481 tcgtgatcac ttccgagctg cccccgcgct tcccggtgga ggcgctggcc gccctcgcgg
      541 cgaccctggg aagagagctg gagttgggcg cctcccgcct cgagcgcagc gccttccgga
      601 ccgcgctgtt ctcggcggac gacggaaccg tcttcatcgg aggcagcccg gtcggttacg
      661 tcaccctggt ggcggaccgc aacgtgaaca tggaggcggt tcgcgccgcg ctcggccgcg
      721 cggtggatcg cctgcgcggc acctggcgga ccgccgcctg agcccgatct aggtcccgag
      781 ggtcacccgc aggacctcct ccaccgtcgt tacgccgtcg atcaccttct gtagcgcgtt
      841 ctggcggagg gtcttcatgc cctgcgagca ggcggcgtcc tggatctcgt tggtggtcgc
      901 gttacgcaag atcaggtcgc ggatctcggt gctcaccggc atgacctcgt agatggccac
      961 ccgacccttc atcccggtga agttgcaggt ctggcagccc ttgcccttgt agaagttcac
     1021 ggtgccgagc ccctggggca cgtgcccgta gggcaccagg ctctcctcgc tcgcctcgta
     1081 gggctccttg cagtcgcgac agacgcggcg cccgagccgc tgcgccagga tcagctgcag
     1141 cgacgaggag accaggaagg gcgggatgcc catgtccagg agccgcgcgg gggtcgacgg
     1201 gcaatcgttg gtgtgaaggg tggacagcac caggtggccg gtcagcgcgg cgcggatgcc
     1261 gatctgggcg gtctccaggt cacgcgtctc accgaccagg atcacgtccg ggtcctggcg
     1321 caggaagctc cgcaggcacg ccgcgaaggt gcgcccgatc tcgtcgttga tctgcacctg
     1381 gttgacgccc ttcaggttgt actcgaccgg gtcctccgcg gtcatgatgt tgatgtccgg
     1441 ggagttgatg gtgtggatcg ccgaatagag ggtcgtggtc ttgccggacc cggtcggccc
     1501 ggtgatcagg atcatgccgt agggactgtg gatcgccttg gtgaactgct ccatgctcca
     1561 gggatcgaag ccgagcgcgg tcaggtcgag ctgcagcgcg tccttgtcca ggatacgcat
     1621 gaccgtcttc tcgccgaaga tcgtcggtag cgtcgagact cggaagtcga tctcgcgctg
     1681 gtggtagcgc agcttgatgc gcccgtcctg gggaagccgc cgctccgcga tgtcgagggt
     1741 ggccatgatc ttcacgcgcg aggtcagcgc ggcctcgagg cgcttgggtg gggtcatgat
     1801 ctcgtggagc acgccgtcga tgcggaaccg gacccggaac accttctcgt acggctccag
     1861 gtggatgtcc gaggcgcccc gacggatggc gtcggtcagg atcatgttga tgagccggac
     1921 caccggcgct tcgtcggccg actccttgag gtcgaagacg tcggccttgg tgaaggtctc
     1981 ctcgccgccc tcgacgacct cgacgtcggt gtcggcctcc tcgagctcgc tcatcatgtc
     2041 ggtgacgctg ccgccggtct cgtagagacg ctcgaacgcc ttgcggatgg ccgcctccga
     2101 ggcgaccacc ggctggatct gcaacccggt catgaagccg acgtcgtcga gcgcgaagac
     2161 gttggtgggg tcggccatcg cgagggtgag gacgttgccc atccgcttga tgggcagcac
     2221 gctgtgcttc ttggcgatct gctcgggcac cagcttcagc acgtccggat ccggatcgac
     2281 ctgcgccacc gtgatggcgg ggatcgagta ctgcttggac aggaaggaga cgagactatc
     2341 ctcggtgatg aagccgagtc ggaccaggat ggtccccagc ttgtccgcgc tccccttctg
     2401 ctcggcgagg gcccgttgca gctgctcggt cgtgatcagc ttctcggcca ccaggcgatc
     2461 gccgagacgg gacaggctgc cctgccgtcg ctcgctcgcc accgcggtgg cgctccgctt
     2521 gcccggcccg cgcccgagtc cgttgtcccc acccggcatg cccgggtcca gctcgatcgt
     2581 cgcgggcgaa agcggcgccg ccgcggccgg caccggcgcg gcggccggct cgggcgccgg
     2641 ggtcacgctc gcggaggggc gtcctccgac cgtgccggcg agacgcgcga cgacccgggc
     2701 caggttctcc cgatcgccga gcttggtgta gatctccttc agcgtctcga gcgccccggt
     2761 gtccgaggga ttggcctcca cgatcatctc gcagagccgg gccgactcgg ccagggagcg
     2821 cttctcttcg ggcgacagtg gcttgctctc gatctgagcc atgagtggta tccgtcagct
     2881 cagaccgggc gctcccggag agtggactgc acctcgtcct tgtgcagcgg tgccgcgagc
     2941 gctccggtgc ggtgcggacg ggtacgggtc tccgccagga tcgccccgat ggcggtcccg
     3001 gcctccttgg cgaagaggcg caccataccc accgtggtcc gactgtcgaa gatggcgagc
     3061 aggatcgcgt gctcgtccac cgccgccacg tggatgctct ccttcacgcc ttgatgaaac
     3121 agcatcgtga actcggtctc gcccagcagg cgcgccatcg cggcggtcga gctaaaggca
     3181 cccgccgcga gagcggagag cgacaccgtg tcgagcgacc gggacgcgcc gccgcgggcg
     3241 aggagctgcc cgctccgatc gatcaggagg gcctccatcg ctccggactc tcccaggaag
     3301 tgcaggagga cgccgttgat ccgctgcgcg tcctcctcgt ggatggccag attgtcattt
     3361 agactcattg agcgccccct gttcttgtcc gaaacccgcg acgaggacca tgcgcgacac
     3421 ggtgttgagc gtgtcgaaga cccctttgcc ctcgacggcg accgactcga agtacggaac
     3481 ccgccggccc ctgcgattca gcaggtattc gaggtattct accggcgcca cgttgggcag
     3541 gtcccgcttg ttgtactgga tcacgtacgg gatctcatca agattgtacc cgtactcctt
     3601 tagattctct tccaggtttc ggaagctctc gacgttctcg cgcaggcggt cccactgcga
     3661 gtcggcgacg aacacgatgc cgtcgacacc acgcaacacg agcttgcggg tcatgttgta
     3721 gtacacctgc ccgggtacgg tgtagagctg gaacttcgcc acgaagttcc gtaccgtcac
     3781 cgcggagacg ggcaggaaat cgaagaagag ggttcggtcg tctccggtcg cgagcgacac
     3841 catgttgccg cggttcacgt ctggcagccc ccggtagatg tactggaggt tggcggtctt
     3901 gcccgcgacg ccggtgccgt agtacacgac cttgaagctg atctctttct gcgcgtagtt
     3961 gatgatggac atcgatcgcc tcaggcgtgg gccagcatcg cggtcgctcg ctcgacctgg
     4021 cgataggcca ggccggggcg ggtgacgtcc ccacccgcga caaccacgat ggaccgaccg
     4081 tgcccgacct cctcgggctg gatgaccaca agcgtgttcc ccgatctcag cacagcagtg
     4141 cggaacacgg cgccggaccc cgcagccttc cggatgacgg cctggagatc ctgcgcgaac
     4201 gctcccaccg cgggcacatc ggctcctcgc ggcaggaaga agtagaagac cccttcaccc
     4261 tcggtgtccc ggagcaccga cgcggtgacc ggaccgtagg cggtgaaact cgacgcgtag
     4321 cgagtgacct ggtccggcac cgcggccggg acgaggttgc gagtccgatc cgggggcgcg
     4381 ggcaccgagc ccactcgccg actcccctcg gcgccgacgc gccggcagag tatctcgagc
     4441 aaggccaggg atccgccccg gggcgccgcc gtcgccagca cggacgcagc cgagctcccg
     4501 cggctgagcg gcgtcaggac cagcgcggtc tccggaccgc gtagcgtgat ctgatcgaga
     4561 ggccacggtg cccgccggtc tgtcagcagg ggcagcgcga gcccggtcgc cgcgaccgcg
     4621 gtctcgggcg ccaccgcggg cgcggccatc gcgaagacgg tgacgccctc catcggctgg
     4681 atggtcacct cgaacgaggc gattggcgcc aagagggtga ggattcgccg cgcctcggcc
     4741 gccgcggccg gatccgcgag cggggtcgcc ggcgtcggct ccacggatgg ggcgacgcgc
     4801 cactcccgcg ccgtcttagc gacgatcggc tccggcaccg gctccttcca gcgcggctcc
     4861 ggcgttgtgg atggcccgac gtgcaagccc gtcgggctcg ccgccacgag caccggagcg
     4921 ggcgtcacga tgagcggcgc cgcggccgga atgttggtcg catcgggctc ggggtggatt
     4981 tcgattcggc ggctcacctc cgaggcaaca gccgtcggtg gctcggccct gagcgcgggc
     5041 tccgacacga tcgggagctc gggctcgggg acacccggtg gctccgtggc cggaatcgac
     5101 gtcgcgacga cgggcggaag cgcgacgacc tcttgaatcg gcggggcaac caccggcgcg
     5161 aggtctaccg agacggggtc gctcaggcgg ggcggctcgg ccaccagcgg tgaagccgcc
     5221 tggaccacga gcggcggcgc ggccatcggc ttcggctcgg gcggccactc ggagacgctc
     5281 tcggagcccg tggcggacgc cgacggctcc ggcaccaccg acggggccgg ctcgggaaga
     5341 gccacgacgg ctgacgtggc aggttccacc gcagccaccg gctcgggctc cggggccggg
     5401 tccgagacga gcggctggaa cggcgcggga aagctctcca gcccactcac atcgaccgcc
     5461 ggtcccccgc tcatgaacag gtcgggtgaa atttgacgga tgatctcgtc gagtgggaga
     5521 tggatgccat tcgggagccg atcggccatc tcggcatccg acatcgccat ctgctcacgc
     5581 gggaactgtg acgcgaccac gtcccagccc acctggatca atccctcgcc cagctgcggc
     5641 agcacctgcg ccaacggaat gagcagcgca cccggatgct gcatccgcgc ccccagctga
     5701 tcgagcgccc catgaaaccc ttccgccgga agctcggcca tcacgcggcc gaaggaaatg
     5761 cggaccgtgg gctcgtcgat ctgggagacg ggcacccgag cagccactgg ctctggctcg
     5821 ggaacgtgga ccggccccgg cagcggagcc ggctcgggga cgaaccgagc cggctcctcg
     5881 atccgccccg gcagctcgag aacgggagcc ggcgccggct ggaccctcgg ctcggccggg
     5941 agctcgacga cgggccgctc cgtcacgata gg
//
LOCUS       DATMCV010000838         2747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_708837, whole genome shotgun sequence.
ACCESSION   DATMCV010000838 DATMCV010000000
VERSION     DATMCV010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708837"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VNU03_19325"
     CDS             <1..936
                     /locus_tag="VNU03_19325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP76681.1"
                     /translation="VILMYLPLFHAFGFIEGPLMSMIRGARQVLTESFDGGHCLDLIA
                     SEKATIIHGFDTHYKELLDAQEQSPRDVSSVRTGICGTGMSSSIPIARRARKTFGNLM
                     TGFGMSEVGIGVTFSFLDSTEEQCVEANGYPGSGYEVRIIDPETGVDQPVSVPGEILV
                     RGYIVTAGYYKRPEETARAIDADGWFHTGDMGLLRPDGHMRFLGRYKDMLKIGGENVD
                     PMEVEAFLMTHPAVNAASVVGLPDTRLSEVAVAFVQVTAGCALSEQEVIEHCRGRVAS
                     FKIPRHVVFVDEFPMTSSGKVQKVKLRERARQEWP"
     gene            complement(933..1343)
                     /locus_tag="VNU03_19330"
     CDS             complement(933..1343)
                     /locus_tag="VNU03_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWP76682.1"
                     /translation="MAHWLLKSEPEDWSWAQQVAKGRQGAEWTGIRNFSAQNHLRAMK
                     KGEQAFFYHTGKERAIVGIVKVIAEAHPDSTDAAWRAIDVAAVEPLPTPVGLDRIKAD
                     KRFATMALVRLARLSVQPVTDAEWRLICQMGGRG"
     gene            complement(1410..2417)
                     /locus_tag="VNU03_19335"
     CDS             complement(1410..2417)
                     /locus_tag="VNU03_19335"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWP76683.1"
                     /translation="MPALAAAVRWLGPGSRLPAGSADQPWLLVPAGRLVHTRVLRDLI
                     EGAATSERVIMTTSALDPAPVAVLPRAIIGALWSRLAAGSPVGPDLARLLQEWGAQPR
                     ESTDPLVTVGDETARSRAEDALFGDLGIEADTGLDRHFHRRCSRWITRLLVNTPVTPS
                     QVSLASLAVGIAAIWCFWRATPLSAVWGVVFYAIATILDHTDGEIARLTFQESQSGAN
                     LDWTVDTIIHAGLVLGMAVTAEGRPMHWVGLFSALGVTLSALFARYLPLGVVAGGGAG
                     GMLKALGSRDLVYLLLLSFVAFRWLAPSLLQPLAVVVGIGSQAYWIACLVRILRERAT
                     R"
BASE COUNT          450 a          929 c          992 g          376 t
ORIGIN      
        1 gtgatcctca tgtacctgcc gctcttccac gccttcggct tcatcgaggg gccgctcatg
       61 tcgatgatcc ggggcgcccg tcaggtgctg accgagagct tcgacggggg ccactgtctc
      121 gatctcatcg cgagcgagaa ggcgacgatc atccacggct tcgacacgca ctacaaggag
      181 ctgctggacg ctcaggagca aagcccgcgg gacgtgtcga gcgtgcgtac cgggatctgc
      241 ggcaccggca tgtcgagctc gatccccatc gcccgtcgcg cccgcaagac gttcggcaat
      301 ctcatgaccg gattcgggat gagcgaggtc ggcatcgggg tgaccttctc cttcctcgac
      361 tcgaccgagg agcagtgcgt ggaagccaac ggctatcccg gctccggcta cgaggtgcgc
      421 atcatcgatc cggagaccgg agtcgaccag ccggtgtcgg tgccgggtga gatcctggtc
      481 cgcggataca tcgtcacggc gggctactac aagcggccgg aagagacggc gcgggccatc
      541 gacgccgacg gctggttcca caccggcgac atgggcctgc tgcgtccgga cggccacatg
      601 cggttcctcg gccgctacaa ggacatgctc aagatcggcg gggagaacgt ggatcccatg
      661 gaggtcgagg ccttcctgat gacccacccc gccgtgaacg cggcgtcggt ggtcgggctg
      721 cccgacacga ggctctccga ggtggcggtg gccttcgtgc aggtgacggc cgggtgcgcc
      781 ctgtccgagc aggaggtgat cgagcactgc cgcgggcggg tggcgagctt caagattccc
      841 cgccacgtcg tcttcgtcga tgagttcccg atgaccagct cgggcaaggt ccagaaggtc
      901 aagctgcgcg agcgggcgcg gcaggagtgg ccctagccgc ggccccccat ctggcagatg
      961 agccgccact cggcatcggt caccggctga accgatagcc gggccagcct gaccagggcc
     1021 atggtggcga agcgcttgtc ggccttgatc cgatcgaggc cgaccggggt cgggagcggc
     1081 tcgaccgccg cgacgtcgat ggcgcgccag gcggcatcgg tcgaatccgg gtgcgcttcc
     1141 gcgatcacct tgacgatgcc cacgatcgcg cgctccttgc cggtgtggta gaagaacgcc
     1201 tgctcaccct tcttcatcgc gcgcaggtgg ttctgggccg agaagttgcg gatgcccgtc
     1261 cactccgcgc cctggcggcc cttcgcgacc tgctgggccc acgaccaatc ctcgggctcg
     1321 gacttcaaca gccagtgcgc catggcagcc ccgcctcctt ggccgggcac gcccgctagg
     1381 gtagcaaatc gccatgcgcg acgccgacgt caccgagtcg cgcgctcccg gaggatacgg
     1441 accagacatg cgatccagta ggcctgcgag ccgatcccga cgaccactgc cagtggttgg
     1501 aggagggacg gcgccagcca gcggaaggcc acgaagctca gcagcaggag gtacacgaga
     1561 tcgcggctgc cgagggcttt cagcatcccg ccggcgcccc cgccggccac gaccccgagg
     1621 gggaggtacc gggcgaagag cgcgctgagg gtgaccccga gggcgctgaa gaggccgacc
     1681 cagtgcatcg ggcgtccctc cgctgtcacc gccatgccga gcacgaggcc cgcgtggatg
     1741 atcgtgtcga ccgtccagtc gagattggcg ccggactgcg actcctggaa ggtgagccgc
     1801 gcgatctcgc cgtcggtgtg gtccaggatg gtcgcgatcg cgtagaagac caccccccag
     1861 accgcgctga gcggggtggc gcgccagaag caccagatgg cggcgatccc gaccgccagg
     1921 ctcgcaagac tgacctggct cggggtgacc ggggtgttga ccagcagccg ggtaatccag
     1981 cgggagcagc ggcggtggaa gtgacgatcg aggcccgtgt cggcctcgat gccgagatca
     2041 ccgaagagag cgtcctcggc acgggagcgg gccgtctcgt ccccgaccgt gacgagcggg
     2101 tcggtggact cgcggggctg ggcgccccat tcctggagga gccgagccag atccgggccc
     2161 accggggagc cagcggccag ccggctccag agcgccccga tgatcgcccg cggcagcacg
     2221 gccaccgggg ccgggtcgag agccgaggtt gtcatgatca cgcgctccga ggtcgcggcc
     2281 ccctcgatca ggtcccgcaa gacgcgggta tgcaccaggc gcccggcggg gaccagcagc
     2341 cagggctggt cggctgagcc agccggcagt cggctgccgg gcccgagcca gcggacggcg
     2401 gcggccagcg ccggcatgtg tgcgagcgcc cgctcgacgg accggtcacg gagggcggtt
     2461 ggcaccgcga tggtggaagc gccggcccgc agcgcggcga ccaagttccg gaccgtgacg
     2521 gtacggccgg ctacggaacg cgccgcgagc cgcacggagg cggcatccgg gaggtacaga
     2581 agggccgcac ccggtcgaac cgtgcgcgcc ggtccggtcc aagccgggcg aagcgttccc
     2641 gcggatggtg ccttcatcac ggcgacgggg tcccgaagtg atgggcccgc cagctctcga
     2701 agacgaggag cgaccagagg atccgccggt ggtctcgaac gccaccg
//
LOCUS       DATMCV010000839         3504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_179071, whole genome shotgun sequence.
ACCESSION   DATMCV010000839 DATMCV010000000
VERSION     DATMCV010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179071"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VNU03_19340"
     CDS             complement(<1..830)
                     /locus_tag="VNU03_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76684.1"
                     /translation="MAASASPAVSGTAPALGVRRLRKAFGPVIAVDGVSLDAASGEFL
                     ALLGPSGCGKSTLLRMVAGLVEPDDGEIVLAGEDITRVAVHRRNLGLVFQSYALFPHM
                     TVFENVAFGLRRRSVPPAELGPRVERMLELVRLGPLGSRHPRELSGGQQQRVALARAL
                     VTEPKVLLLDEPLSNLDALLRDEMRVELKRLQERLGTTMIFVTHDQAEALILSDRVVV
                     MEAGRVEQIGRPEEVYRRPATAFVARFLGRANFLAGTVAETGPTATVVALDGGGFSVV
                     A"
     gene            complement(838..1860)
                     /locus_tag="VNU03_19345"
     CDS             complement(838..1860)
                     /locus_tag="VNU03_19345"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76685.1"
                     /translation="MTVLRATVLMCLLSVGLVRPAAADELVVATFGGTFVDNSKKCHA
                     AAFEKATGSTVKYVLGSSVQTMAKLRAAGARAEFDVAYMDSQIVKQAKAEGLLQPLEP
                     AKIEHWNDLYDASRDKDAYWVSMMFAGTIITYNTNLVKTPPTSWADLWKPEWKGKLAI
                     PDISGTSGQQFLMAAARLNGGSVENIDPGFEAIKKLKPNIQMMYTQPDQIIPLFERGD
                     IALAVWYTDRTGAAALKGVPVAAAYPSEGAIGIVPTVSVPKGSQKRELAQKYIAVLLA
                     PEGQLCFGQSQFAGPTNKKVQLPADLAKLVPYGPLVQRMFFPDTDLVAKKFPEWSERW
                     GREIAR"
     gene            1999..>3504
                     /locus_tag="VNU03_19350"
     CDS             1999..>3504
                     /locus_tag="VNU03_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76686.1"
                     /translation="MTDRSEDSIQLPPDPTRRAFLGAGALTLLGLAAPTVPLAGAQTP
                     KRGGTLVVAADVSPPGLDPQKSAAAHSWMIAEHVYSTLLRRDPKMNIVGDLAESWQAV
                     NDTTFVFKLRKGVAWHHGRDLVADDVKYSFERMLDEKTASPWRSNWSIIDRIEAPDRA
                     TVRFVIKRPFAPFFSYLATPHYGAIVPRDIVEKNGDLQKVASGTGPFVLERFVPDNTV
                     VLKRNPSYYESGLPYLDGLEYRIIPDEAARLAALRAGTVHYLWSVDPLIDRQLQGATG
                     VILREPDGYCAQHGMAFNQTKPPFTEVAVRRAVSVGINRREIIASVLRGHGAISTKIP
                     PCDAPFGYSGDEKGLPYYAHDAALSRRLLAEAGFPNGLDTTLEVSPRFPQTVRTGEVM
                     KEQLAAAGIRVTLKQTEWGAALKNFVRTEYEGMSMIPLVWQPDPDAHVYDIFHSGSAI
                     NLGKFKDPRVDQLLEQGRTTLDRDKRIAIYRDLQRHMADQAYLVFPYASGAT"
BASE COUNT          535 a         1290 c         1183 g          496 t
ORIGIN      
        1 gccaccaccg agaacccgcc gccgtccagc gcgacgacgg tggcagtcgg cccggtctcc
       61 gccacggtgc ccgcgaggaa gttcgcgcga ccgaggaagc gcgcgacgaa ggcggtggcc
      121 gggcggcgat agacctcctc cggccggccg atctgctcca cgcgcccggc ctccatcacc
      181 accacgcggt ccgacaggat gagcgcctcc gcctggtcgt gggtgacgaa gatcatcgtg
      241 gtcccgaggc gctcctgcag ccgcttcagc tcaacgcgca tctcgtcccg cagcagcgcg
      301 tccaggttgg acagcggctc gtcgaggagc agcaccttgg gctcggtgac gagcgcgcgg
      361 gcgagcgcca cgcgctgctg ctggccgccg gagagctcgc gcggatgccg gctcccgagc
      421 gggccgaggc gcaccagctc cagcatgcgc tcgacccggg gccccagctc ggccggcggc
      481 accgagcgcc ggcggagtcc gaaggcgacg ttctcgaaca cggtcatgtg gggaaagagg
      541 gcgtaggact ggaagacgag cccgaggttg cggcgatgga ccgccacccg ggtgatgtcc
      601 tcgcccgcga ggacgatctc cccgtcgtcg ggctccacga ggcccgccac catgcggagc
      661 agcgtgctct tgccgcaccc cgagggccca aggagcgcga ggaactcgcc cgatgcggcg
      721 tcgagcgaca cgccgtcgac cgcgatcacc gggccgaacg ccttgcggag ccggcggacg
      781 ccgagcgccg gcgcggtgcc gctcaccgcc ggggacgcgc tcgcggccac cccggggtca
      841 acgcgcgatc tcgcgccccc agcgctccga ccactccggg aacttcttgg ccaccagatc
      901 ggtgtcgggg aagaacatgc gctgcacgag cgggccgtag ggcacgagct tcgccagatc
      961 ggccgggagc tgcaccttct tgttggtggg ccccgcgaac tggctctggc cgaaacagag
     1021 ctggccctcc ggcgccagga ggaccgcgat gtacttctgg gccagctccc gcttctggct
     1081 ccccttgggc accgagacgg tcggcacgat gccgatcgcg ccctcgctcg ggtaggccgc
     1141 cgccaccggc acgcccttca gggcagccgc gccggtccgg tccgtgtacc acaccgcgag
     1201 cgcgatgtcg ccgcgctcga agagcgggat gatctggtcc ggctgcgtgt acatcatctg
     1261 gatgttgggc ttgagcttct tgatcgcctc gaacccggga tcgatgttct cgaccgagcc
     1321 gccgttcagg cgcgccgccg ccatcaggaa ctgctggccc gaggtgcccg agatgtcggg
     1381 aatcgcgagc ttgcccttcc actccggctt ccagaggtcg gcccacgagg tgggcggcgt
     1441 cttcacgaga ttggtgttgt aggtgatgat ggtgcccgcg aacatcatcg agacccagta
     1501 ggcgtccttg tcccgcgagg cgtcgtagag atcgttccag tgctcgatct tcgcgggctc
     1561 gagcggctgg agcaggccct ccgccttcgc ctgcttgacg atctggctgt ccatgtaggc
     1621 gacgtcgaac tcggcccggg ccccggccgc gcgcagcttg gccatggtct gcaccgagct
     1681 gccgagcacg tacttcacgg tggagccggt cgccttctcg aaggcggcgg cgtggcactt
     1741 cttcgagttg tcgacgaagg tgccgccgaa cgtcgccacc accagctcgt cggcggcggc
     1801 gggccgcacg aggccgacgg agagcaggca catcagcacg gtcgctcgga gcacggtcat
     1861 gatcgctggt cctctcggga tacggggtgg cgcgattatt cgccaggcgc gccgggcagt
     1921 caacccttga cgcgtccatt tccgcgggcg agtatggcgc tcggcagttc accggcaccc
     1981 gagagcccgg agggcggcat gaccgaccgc agcgaggact cgatccagct cccgcccgac
     2041 cccacccggc gcgcgttcct cggcgcaggc gccctcaccc tgctggggct cgcggcgccg
     2101 accgtcccgc tcgccggggc ccagaccccg aagcgcggcg gcaccctggt cgtcgcggcc
     2161 gacgtcagcc cgcccggcct cgacccgcag aagtccgccg cggcccacag ctggatgatc
     2221 gcggagcacg tctactcgac cctgctccgc cgcgatccga agatgaacat cgtgggcgac
     2281 ctcgccgagt cctggcaggc ggtcaacgac accaccttcg tgttcaagct gcgcaagggc
     2341 gtcgcgtggc accacgggcg cgacctcgtg gccgacgacg tgaagtactc gttcgagcgg
     2401 atgctcgacg agaagaccgc gtcgccctgg cgctccaact ggtcgatcat cgaccgcatc
     2461 gaggcgcccg accgggccac cgtgcgcttc gtcatcaagc ggccgttcgc ccccttcttc
     2521 tcctacctgg ccacgcccca ctacggggcc atcgtcccgc gcgacatcgt ggagaagaac
     2581 ggcgacctcc agaaggtcgc gtccgggacc gggcccttcg tcctcgagcg gttcgtcccg
     2641 gacaacacgg tcgtcctcaa gcgcaacccc agctactacg agtccggcct cccctacctc
     2701 gacgggctcg agtaccggat catcccggac gaggcggcgc gcctggccgc cctgcgggcg
     2761 ggcaccgtcc actacctctg gtccgtcgac ccgctcatcg accggcagct ccagggcgcc
     2821 accggggtga tcctccgcga gccggacggc tactgcgcgc agcacggcat ggccttcaac
     2881 cagacgaagc cgccgttcac cgaggtggcg gtccgccggg cggtcagcgt gggcatcaat
     2941 cggcgcgaga tcatcgcgag cgtgctgcgg ggccacggcg cgatcagcac caagatcccg
     3001 ccctgcgacg cgcccttcgg ttacagcggc gacgagaagg gcctccccta ctacgcccac
     3061 gacgcggccc tgtcgcggcg cctgctcgcc gaggcgggct tccccaacgg cctcgacacg
     3121 accctcgagg tgtcgccccg cttcccccag acggtgcgga ccggcgaggt catgaaggag
     3181 cagctcgccg cggccggcat ccgcgtgacg ctgaagcaga ccgagtgggg cgcggcgttg
     3241 aagaacttcg tgcgcaccga gtacgagggc atgagcatga tcccgctggt gtggcagccc
     3301 gaccccgacg cccatgtcta cgacatcttc cacagcgggt cggcgatcaa cctgggcaag
     3361 ttcaaggacc cgcgggtcga ccagctcctc gagcagggcc gcaccacgct cgatcgggac
     3421 aagcggatcg cgatctaccg ggacctccag cgccacatgg ccgaccaggc ctacctggtc
     3481 ttcccgtacg cctcgggcgc gacg
//
LOCUS       DATMCV010000840         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_43884, whole genome shotgun sequence.
ACCESSION   DATMCV010000840 DATMCV010000000
VERSION     DATMCV010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43884"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VNU03_19355"
     CDS             <1..353
                     /locus_tag="VNU03_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007950829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76687.1"
                     /translation="LIPETRHLINAQSLRTMKKTAFLINAARGPVVDEAALVQALKEG
                     SIAGAGLDVFEEEPKVHPGLIGLPTVVLAPHIASASSETRLKMANLAVENCLAVLEGK
                     TPPTPVNPEVLSRR"
     gene            356..1393
                     /locus_tag="VNU03_19360"
     CDS             356..1393
                     /locus_tag="VNU03_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76688.1"
                     /translation="MDLLVLDRVSKRFEPDVSPAVDGLSLRVAEGEILALLGPSGCGK
                     TTTLRLIAGLETPDSGTITLRGRPVAGPGHAVPPEERGVGIVFQDYALFPHLTVGDNV
                     GFGLARAARRDRVADVIDLVGLGGLAARYPHELSGGQQQRVALARALAPAPAVMLLDE
                     PFSNLDADLRAQMRDEVERILRSSATTAIFVTHDQEEAFTLADRVGVLRAGSVEQLAT
                     PSQIYHRPATRFVASFVGAADFLPGVVTGLGIVTEVGTFANVEQLALGGRVDVMIRPD
                     DITFVPETDGAGTITRRYFRGSETVYCIRLPSGHRVHSSQPSASILPAGMRVRVSSHV
                     LHVVAFPAVDT"
     gene            complement(1390..>2279)
                     /locus_tag="VNU03_19365"
     CDS             complement(1390..>2279)
                     /locus_tag="VNU03_19365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP76689.1"
                     /translation="LFALVLLLLEHRSRGRARFVQRGRASRALEAEPLRGFRAVAATA
                     VCLAVLGPAFVLPVGQLAWWALAAVRGGRVAPDFLALLEHTALLAAAAAGLACALAVL
                     LAYAGRLRGTRVVRLAARLASMGYALPGAVIAVGVLLPLAWADHVLVPHLERVLDRPL
                     GLLLTGSAAGLILAYVVRFLAVGLQSVEASLGRIPPSLDDAARTLGARAGVALRRVHL
                     PLIRGGLLTALVLVFVEAMKEMPATLLLRPFGLNTLAIEIWERTSEAMWQEAAVPALA
                     LVGVGLLPVMLLIRLTSRG"
BASE COUNT          324 a          879 c          806 g          270 t
ORIGIN      
        1 cgctgatccc cgagacccgt cacctgatca acgcccagtc gctccgcacc atgaagaaga
       61 ccgcgttcct gatcaacgcg gcccgcggcc cggtggtgga cgaggcggcg ctcgtccagg
      121 ccctcaagga aggctccatc gcgggggccg gtctcgacgt gttcgaggag gagccgaagg
      181 tgcacccggg cctgatcggg ctgccgaccg tggtgctggc cccccacatc gcgagcgctt
      241 cttccgagac gcggctcaag atggccaacc tcgcggtcga gaactgcctc gccgtgctcg
      301 agggtaagac gccgcccacg ccggttaatc ccgaggtgct gtcgcgccgg tagccgtgga
      361 tctgctggtc ctcgatcggg tcagcaagcg attcgagccg gacgtatccc cggcggtcga
      421 tgggctgtcg ctccgggtgg ccgagggcga gatcctggcc ctgctcggcc cctcgggctg
      481 cggcaagacc accacgctgc gcctcatcgc gggcctcgag accccggact cgggcaccat
      541 cacgctgcgc ggccgccccg tggccgggcc gggccacgcg gtgccacccg aggagcgcgg
      601 cgtcggcatc gtgttccagg actacgccct gttcccgcac ctgaccgtgg gcgacaacgt
      661 gggcttcggc ctggcccggg ccgcccgccg cgatcgggtc gccgacgtca tcgacctggt
      721 cgggctcgga ggcctcgccg ctcgctaccc ccacgagctc tccggcggcc agcagcagcg
      781 cgtcgccctc gcgcgcgccc tcgcccccgc ccccgcggtc atgctcctcg acgagccctt
      841 ctcgaacctg gacgccgatc tccgggccca gatgcgcgac gaggtggagc ggatcctccg
      901 ctcgagcgcc accaccgcga tcttcgtgac ccacgaccag gaggaagcgt tcaccctggc
      961 ggaccgggtc ggcgtcctgc gggccggctc agtcgagcag ctcgcgaccc cgtcgcagat
     1021 ctaccaccgc ccggccacgc gcttcgtggc ctccttcgtg ggcgcggccg acttcctgcc
     1081 cggggtcgtc accggcctcg gcatcgtcac cgaggtgggc acgttcgcca acgtcgagca
     1141 gctcgcgctc ggcgggcggg tcgacgtgat gatccggccc gacgacatca cgttcgtccc
     1201 ggagacggac ggggccggca ccatcacccg ccgctatttc cgcgggtcgg agacggtgta
     1261 ctgcatccgg ctgccgtcgg gccaccgggt gcactcctcc cagccctcgg cctcgatcct
     1321 gcccgccggc atgcgggtcc gggtctcgtc acacgtgctc cacgtggtgg cgttcccggc
     1381 ggtcgacacc tagccgcgag acgtcagccg gatcagcagc atcaccggca gcagccccac
     1441 tccgaccagg gccagcgccg gcaccgccgc ctcctgccac atcgcctccg acgtccgctc
     1501 ccagatctcg atggcgagcg tgttgagccc gaacggccgg agcagcaggg tggccggcat
     1561 ctccttcatg gcctcgacga acaccagcac cagcgcggtc aggagtccgc cccggatgag
     1621 cggcaggtgg acgcggcgca gggcgacgcc cgcgcgggcc ccgagtgtcc gcgccgcgtc
     1681 gtcgaggctc ggcgggatcc ggccgaggct cgcctccacc gactgcagtc ccaccgcgag
     1741 gaagcggacg acgtaggcga ggatcagccc cgccgccgac ccggtgagga gcaggccgag
     1801 cgggcgatcg aggacgcgct cgaggtgggg caccaggacg tggtcggccc aggcgagcgg
     1861 caggagcacc ccgaccgcga tgaccgcgcc gggcagcgcg tagcccatgg acgcgagccg
     1921 ggccgcgagc cggaccacgc gggtgccgcg gagccgtccc gcgtaggcga ggagcacggc
     1981 cagggcgcac gcgagccccg ccgcggcggc ggcgagcagc gcggtgtgct cgaggagcgc
     2041 gaggaaatcg ggcgcgacgc gaccgccgcg caccgcggcg agggcccacc acgccagctg
     2101 ccccaccggc agcacgaagg ccgggccgag gacggccaaa cacaccgcgg tggcggccac
     2161 cgcccggaag ccccgcagcg gctccgcctc cagcgcgcgg ctcgcccggc cgcgctggac
     2221 gaagcgggcc cggccccgcg agcggtgctc gagcaggagc aggaccaggg cgaacagca
//
LOCUS       DATMCV010000841         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_777269, whole genome shotgun sequence.
ACCESSION   DATMCV010000841 DATMCV010000000
VERSION     DATMCV010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777269"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VNU03_19370"
     CDS             <1..291
                     /locus_tag="VNU03_19370"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HWP76690.1"
                     /translation="AGADIVGMNCGRGPDRAIVIIREMRQVTDAPLIAYPNAGLPITT
                     GGRTTYELGPEAMAKDYPALLDAGCNIVGGCCGSNPEHIRLIADVVRSRRAR"
     gene            288..1043
                     /locus_tag="VNU03_19375"
     CDS             288..1043
                     /locus_tag="VNU03_19375"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HWP76691.1"
                     /translation="MTALAFSLLSRTPVVLEGLPLSIDRDEVLRFQGYQKGVDLPSPA
                     VVAIFEEALALGERLMAPRLVYRAAPVDERGADLIVAGGERLGIPQIGRLWGPIDAVG
                     AGICTVGEAIETRVRALFDAREFPLAVMLDSVGSAAAESLAEYANDLLCQAALPQGLK
                     VTNRVSPGYAGWDTAEQARLFRICPGDPIGVQLNEACFMTPAKSISWLVGLGPEARVD
                     HYFTQCRRCWMRDCAYRRAPATVTVQRPGAGVW"
     gene            1059..1589
                     /locus_tag="VNU03_19380"
     CDS             1059..1589
                     /locus_tag="VNU03_19380"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWP76692.1"
                     /translation="MGDNVVLVGFMGAGKSSVGRLLARRMGRCFVETDDMITAREGRS
                     IPEIFAERGEAHFRALEEETVRLLALKSGDVIATGGGLYCRDGRPEALRAIGTVVWLT
                     GSFDVLYERARRMGERPMLAGRTRDEVADIYHAREAYYRQADLTVDTTGRNTDQVVTQ
                     VLLALRPHLSRAEGSR"
     gene            1593..>2074
                     /locus_tag="VNU03_19385"
     CDS             1593..>2074
                     /locus_tag="VNU03_19385"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HWP76693.1"
                     /translation="MPSPLLTRLGERPLLCDGAMGTMLYARGVPLDACFDVLNLNEPK
                     IVQSIHAEYIQAGSDLIETNTFGANRFKLAAHGLQGRVREINLRGAKLARDVRESMGR
                     DVLVVGSIGPLGKYLAPLGSIEPEEARLAFHEQAEGLLEGGVDAFVIETFSDLTEMGL
                     A"
BASE COUNT          279 a          749 c          750 g          296 t
ORIGIN      
        1 gcgggggcgg acatcgtggg gatgaactgc gggcgcggtc ccgaccgcgc catcgtgatc
       61 atccgcgaga tgcgtcaggt caccgacgcg ccgctcatcg cgtaccccaa cgcgggcctg
      121 ccgatcacca ccggcggacg gaccacctac gagctcggcc cggaggcgat ggccaaggac
      181 taccccgcgc tgctcgacgc ggggtgcaac atcgtgggcg gctgctgcgg ctcgaatccg
      241 gagcacatcc gcctcatcgc cgacgtggtg cgctcccgcc gcgcgcggtg accgcgctcg
      301 cgttctccct cctctcccgc accccggtcg tcctcgaggg actgccgctc tccatcgatc
      361 gcgacgaggt cctgcgcttc cagggctacc agaagggcgt cgacctgccg agcccggcgg
      421 tggtggccat cttcgaggag gcgctcgcgc tcggggagcg cctgatggcg ccgcggctgg
      481 tctaccgcgc ggccccggtg gacgagcgtg gcgcggacct gatcgtggcg ggcggcgagc
      541 ggctcggcat cccccagatc ggccgcctgt gggggccgat cgatgcggtc ggggccggca
      601 tctgcacggt cggcgaggcc atcgagaccc gcgtgcgcgc cctcttcgac gcgcgcgagt
      661 tccccctcgc ggtgatgctc gacagcgtgg gctcggccgc ggccgagagc ctcgccgagt
      721 acgccaacga ccttctctgc caggcggccc tgccgcaggg gctcaaggtc accaaccggg
      781 tcagcccagg ctacgcgggc tgggataccg ccgagcaggc gcggctcttc cggatctgcc
      841 cgggcgatcc gatcggggtg cagctcaacg aggcgtgctt catgaccccg gccaaatcca
      901 tcagctggct ggtcgggctc gggccggagg cgcgggtgga ccactacttc acccagtgcc
      961 ggcgctgctg gatgcgcgac tgcgcctacc gccgggctcc cgccaccgtc acggtgcagc
     1021 ggcccggcgc gggggtgtgg tgataggctg gaggtctcat gggcgacaac gtggtgctgg
     1081 tggggttcat gggcgcgggc aaatcctcgg tggggcggct cctcgcccgc cggatgggcc
     1141 gctgcttcgt cgagaccgac gacatgatca ccgcacgcga gggccgctcg atcccggaga
     1201 tcttcgccga gcgcggcgag gcgcacttcc gcgccctcga ggaggagacg gtgcggctgc
     1261 tcgcgctcaa gtcgggtgac gtgatcgcca ccggcggcgg cctctactgc cgcgacggcc
     1321 ggcccgaggc cctccgcgcg atcggcaccg tggtgtggct caccggcagc ttcgacgtgc
     1381 tctacgagcg ggcgcgacgc atgggcgagc gcccgatgct ggcgggccgg acccgggacg
     1441 aggtcgccga catctatcac gcgcgcgagg cctactaccg ccaggccgat ctcaccgtcg
     1501 ataccaccgg ccgcaacacc gaccaggtgg tcacccaggt cctcctcgcg ctccggccgc
     1561 atctctcccg tgccgagggg agccggtagg ccgtgccctc tccgctcctg acccgcctcg
     1621 gcgagcgccc gctcctctgc gacggcgcga tgggcaccat gctctatgcc cgcggggtgc
     1681 cgctcgacgc gtgcttcgac gtgctgaacc tcaacgagcc caagatcgtg cagtcgatcc
     1741 acgccgagta catccaggcc ggctcggacc tgatcgagac caacaccttc ggggccaacc
     1801 gcttcaagct ggccgcgcac ggcctgcagg gccgggtgcg cgagatcaac ctgcgcgggg
     1861 ccaagctcgc gcgggacgtg cgcgagagca tgggccgcga cgtgctcgtg gtgggctcga
     1921 tcgggccgct cggcaagtac ctggcgccgc tcggcagcat cgagcccgag gaggcgcggc
     1981 tcgcgttcca cgagcaggcg gaggggctgc tcgagggcgg ggtggacgcg ttcgtgatcg
     2041 agacgttctc cgacctcacc gagatggggc tcgc
//
LOCUS       DATMCV010000842         2219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_449754, whole genome shotgun sequence.
ACCESSION   DATMCV010000842 DATMCV010000000
VERSION     DATMCV010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449754"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VNU03_19390"
     CDS             <1..226
                     /locus_tag="VNU03_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWP76694.1"
                     /translation="VLFQAAAILVFLVNIMMSLRRGAPAGDDPWDAWTLEWATTSPPP
                     AYNFDVVPVVSSRRPLWDLKHPDDPDGPHE"
     gene            219..767
                     /locus_tag="VNU03_19395"
     CDS             219..767
                     /locus_tag="VNU03_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWP76695.1"
                     /translation="MNERGRVGMIGLIIAESSLFAVFVVAYLFYIGKSLNGPYPKDVL
                     ELPIFNTVCLLSSSITIAVALRALRAGAVRRCGVWLLVTVALGAVFLAGTAREWYGLI
                     VGHGLTIGTNLFGTTFYSLVGLHASHVIVGLVILSLLCVFSFSGALRRKDIERAEIAS
                     WYWHFVDGVWIVVFTVVYLVGR"
     gene            764..1603
                     /locus_tag="VNU03_19400"
     CDS             764..1603
                     /locus_tag="VNU03_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76696.1"
                     /translation="MTPTPAAEPQTIEMPAPTAWPLALALGITLTFAGLVTNEIVSGV
                     GVILTLAAALGWWRQVLPEAKHEYVPMRSVAEQARPIVPSPVAVGRLDLGEGGHRMRI
                     PVEVQPISAGVRGGIVGGAAMAVVALAYGVVFQHSLWYPVNLLSAVAMPGMAQASLAE
                     LRAFSMSALIIGIIAHGVISVLTGLLYAVILPMLPGRHVLWGGLVAPLLWTGGLWAVL
                     GVINPILNARVDWTWFVVSQVAFGLAAGYVVSRAHPVATMQSWPLAARAGVKTSGARE
                     IDR"
     gene            1600..>2219
                     /locus_tag="VNU03_19405"
     CDS             1600..>2219
                     /locus_tag="VNU03_19405"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWP76697.1"
                     /translation="MNAFPRLRAAAGAVVVCLGFAACDALPGRPAIAKREVMPSQVTA
                     FDVLYGRNCAGCHGADGRLGAARSLNDPVYLALVSADRLRTIIGEGVPGTSMPAFRNS
                     SGGDLTGAQVDALVKGMLARWARADAVAGTTLPPYEGPGKGSSTADKARGAVVFASAC
                     ARCHGPEGKGGPTTGSIVDPSYLALVSDQSLRTTVIAGRPDLGKPD"
BASE COUNT          284 a          729 c          803 g          403 t
ORIGIN      
        1 ggtgctattc caggcggcgg caattctcgt gttcctggtc aacatcatga tgtcgctgcg
       61 ccggggggcc ccggcgggcg acgacccctg ggacgcctgg acgctcgagt gggccacgac
      121 ctcgccgccg cccgcctaca acttcgacgt ggtcccggtg gtctcgagcc ggcggccgct
      181 ctgggacctg aagcatcccg acgatccgga cggtccccat gaatgagcgc gggcgcgtgg
      241 gcatgatcgg cctcatcatc gcggagagct cgctcttcgc ggtcttcgtg gtcgcctatc
      301 tcttctacat cggcaagagc ttgaatgggc cgtatccgaa ggacgtgctg gagctcccga
      361 tcttcaatac ggtgtgtttg ctctcgagca gcatcacgat cgcggtcgcc ctccgtgcgc
      421 tccgtgccgg tgcggtccgc cgctgcggcg tctggttgct cgtgacggtc gcgctgggcg
      481 ccgtgttcct ggccgggacc gcgagggagt ggtacgggct gatcgtcggg cacgggctca
      541 cgatcgggac caatctcttc gggaccacgt tctattccct ggtcgggctc cacgcgagcc
      601 atgtgatcgt cgggctcgtc atcttgagcc tgctctgtgt gttctcgttt tccggcgctt
      661 tgcggcggaa ggatatcgag cgcgcggaga tcgcgtcctg gtactggcac ttcgtcgacg
      721 gcgtgtggat cgtcgtcttc accgtggtct acctggtggg ccgatgaccc cgacgcccgc
      781 ggccgagccc cagaccatcg agatgccggc gccgaccgcc tggccactgg ccctcgccct
      841 gggaatcacc ctgaccttcg ccggcctggt gacgaacgag atcgtgtccg gggtgggagt
      901 gatcctgacg cttgccgcgg ccctcggctg gtggcggcag gtcttgcccg aggcgaagca
      961 cgagtacgtg ccgatgcgat cggtcgccga gcaggcgaga ccgatcgtgc catctcccgt
     1021 cgcggtcgga aggctcgacc tcggcgaggg tgggcaccgc atgcggatcc cggtcgaggt
     1081 ccagcccatc tcggcgggcg tcaggggagg gatcgtcggg ggcgcggcga tggcggtcgt
     1141 ggccctcgcg tacggggtcg tcttccagca cagcctgtgg taccccgtga acttgctctc
     1201 cgcggtggcg atgccgggga tggcgcaagc cagcctggcg gagctccgcg ccttcagcat
     1261 gagcgccctg atcatcggga tcatcgcgca cggcgtgatc tcggtgctga ccgggctcct
     1321 ctatgccgtg atcctgccga tgctgcccgg ccggcacgtc ctctggggag gcctcgtggc
     1381 gccgctgctg tggacgggag ggctgtgggc ggtgctgggc gtcatcaatc cgatcctgaa
     1441 cgcccgcgtc gactggacct ggttcgtggt ctcccaggtc gcgttcgggc tggcggccgg
     1501 atacgtggtg tcccgggcgc acccggtcgc caccatgcag tcatggccgc tggcggcgcg
     1561 cgcaggggtc aaaacctctg gcgctcgcga gatcgaccga tgaacgcttt tccgcggcta
     1621 cgcgcggctg ccggcgcggt ggtcgtctgt ctcggcttcg ccgcgtgtga cgccttgccc
     1681 gggcgcccgg cgatagcgaa acgtgaggtc atgccttcgc aggtgacggc gttcgacgtg
     1741 ctctacggac gtaactgcgc gggctgtcac ggtgccgatg ggcgcctcgg cgccgcccgg
     1801 tcgctcaacg accccgtcta cctggccctg gtgtcggcgg accgactgcg cacgatcatc
     1861 ggcgaggggg tgccgggcac ctcgatgccg gcattccgaa acagctcagg aggcgatctg
     1921 acaggcgcgc aggtcgacgc gctggtcaag ggaatgctcg cccgctgggc tcgggcggac
     1981 gcggtggccg gcacgacgtt gccgccctac gaggggcccg gcaagggctc tagcactgcg
     2041 gacaaggccc ggggtgcggt cgtctttgcc agcgcctgcg cgcggtgcca tggaccggag
     2101 ggcaagggtg ggccgacgac gggctcgatc gtggatccgt catatctcgc gctggtgagc
     2161 gatcagtccc tgcggaccac ggtgattgcc gggcgaccgg acctgggcaa gcccgactg
//
LOCUS       DATMCV010000843         6156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_511442, whole genome shotgun sequence.
ACCESSION   DATMCV010000843 DATMCV010000000
VERSION     DATMCV010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511442"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VNU03_19410"
     CDS             complement(<1..240)
                     /locus_tag="VNU03_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP76698.1"
                     /translation="MNFDLTEEQQLLADSLKRYLANEYSIDKRAKIVDSAAGWSETVW
                     GAFAEMGLLGVPFAEEHGGFGGTAVDVMLVMEALGE"
     gene            complement(482..1660)
                     /locus_tag="VNU03_19415"
     CDS             complement(482..1660)
                     /locus_tag="VNU03_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP76699.1"
                     /translation="MDLNYSPEELTFRDEVRGWLRTNLPADLRQKMESYQELSKEELL
                     RWHKILAQKGWVAPDWPQEWGGTDWNVVQRYIFEEECGFAATPMVVPFGVRMCAPVLL
                     QFGTDAQKKRFLPRIYQGEDFWCQGYSEPGSGSDLASLKTRAVRQNGDYVVTGQKIWT
                     TMAQHADWIFCLVRTDASGKRQDGISFLLIDMKTLGITVRPIVLLDGSKEVNEVFFDE
                     VKVPAENLVHEEGKGWTVAKYLLGYERMGTGRIGASKRELAKLKEVASEQMKNGRPII
                     EDPRFRDRLTRVEVELMALEITNLRFLDQMRRSGRPPGADVSMLKIKGTEIQQAITEL
                     MMQAAGPLAQAFRPVDAMDFDHFTARLAPRYCNLRKATIYAGSNEIQRNIIAKATLGL
                     "
     gene            complement(1717..2751)
                     /locus_tag="VNU03_19420"
     CDS             complement(1717..2751)
                     /locus_tag="VNU03_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP76700.1"
                     /translation="MRVSISLRLAQTDWAEASAYVTEAERLGVHDVWSAEAWGHDAVT
                     PLAFMAARTSRIRLGTGIIQAGTRTPALVAMTAMSLASMSNGRFVLGLGVSGPQVIEG
                     WHGIRFDRPVQRMREIVEIVRRAVTGEHLAYKGKLYELPLPGGEGKAIRSAARPQPGI
                     PIYLATLSPKSLEMTGEIADGWLGTSFMPDHAQVFTNHLAAGAARAGRSLAQLDLQAG
                     GFVAFSDDVERLLPPRKPGLAFTLGAMGSRQHNFYNEAFRRAGYEDVAVEVQRLWLAG
                     KREEAAARVPDELVQKTNLLGTEAMVRTRLEAYRRAGITTLRVEPAGEGLAARLATLG
                     RLLDLIRALG"
     gene            complement(2751..3275)
                     /locus_tag="VNU03_19425"
     CDS             complement(2751..3275)
                     /locus_tag="VNU03_19425"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP76701.1"
                     /translation="MDEEQARIRSYLVAQGAKLTPAALVEKVRAAMDDLRAAAVAVPA
                     ERFAERPAPEEWSGNEVMAHVVMADGYFGGGIRDALAGRPAGPRPTRRGVEDAPLQSA
                     QAWYEVLSRQREALFAEVLAADPATAADRRIEHPMFGPLSWRETLLFTRLHDLDHAGQ
                     LQKIAAALGAARPA"
     gene            complement(3385..4140)
                     /locus_tag="VNU03_19430"
     CDS             complement(3385..4140)
                     /locus_tag="VNU03_19430"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP76702.1"
                     /translation="MVVWIHGGSVEDSSVMVADLEPFFDRVRALFPDTRGHGLSSRFE
                     RVEDYTYARKAEDLLHWLDGLGIREAVWGGASMGGALSLWIAVHAPERVRGVVSISGP
                     PFEPTREDKAWWARQRPLVEAGRFAEYFDANVRLRMGEEALARLKARPDRYAELTGAL
                     GRHSVASLLALLDETYQRPEWLPDCRRIRCPVQVIAGSVDSFPTVAMSRRVAETIPGA
                     RLHVVEGGPHFPNRTHRAEVQRVIGTFLTDLGL"
     gene            complement(4217..4894)
                     /locus_tag="VNU03_19435"
     CDS             complement(4217..4894)
                     /locus_tag="VNU03_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWP76703.1"
                     /translation="MGDLDVIWRNFPFLMLQGFLGIGNFVGGTLRLAIPSIILGFLLG
                     IFIGIARLAPQWWIRTPAVLYVEFFRGVPLVMVIFWMWFIMPIVLHTAIPEFGVALAA
                     FVIFEAAYLGEIVRAGIQSVPRGQVEAGTALGLSGSQGMRFVILPQALKNMIPSLVTQ
                     FIVLFKDTSLASIIGFMDLTKAAQVVNNREIRPFPIYLFIAVVYWVCTYSMSRYARYL
                     EKRMGPA"
     gene            complement(4897..5619)
                     /locus_tag="VNU03_19440"
     CDS             complement(4897..5619)
                     /locus_tag="VNU03_19440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWP76704.1"
                     /translation="MRYQFNWSVLWTGESGQWLLSGLITTLELSIVAWLLAAVLGVLS
                     GSLRTVRFWPLRAAATFYVEFFRNVPLLVWMFFWYFAVPPLLPQAVQEWLFAHGLEFW
                     AAVCALGVYSGARFSEVLRSGIQSIPRTQLEASVASGLTTFQAYRYVILPVALRLIIP
                     PGTNESLNLLKNSSVALTISVAELTFQTRQIETYTAKAIEALTAGTLVYLVLCVSIAT
                     IMSWVERHTAIPGLIVGGRQRR"
     gene            complement(5704..>6156)
                     /locus_tag="VNU03_19445"
     CDS             complement(5704..>6156)
                     /locus_tag="VNU03_19445"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWP76705.1"
                     /translation="GSPIKGIKNIDGKRIAAQQGSTNARIIREKAPKAILREFPDQPA
                     AFQALAQGQVDAYTNDGIQLAGLKVKSPNPAQWDIVGDFYSYEPYGMAMRKGESDFRN
                     VVNSGLMDGIESGKFFEIYEKWFGVKGELPYPMDARVRSFMLLQVVPK"
BASE COUNT          995 a         2154 c         2095 g          912 t
ORIGIN      
        1 ctcaccgagc gcctccatca ccagcatcac gtccaccgcg gtgcccccga accccccatg
       61 ctcctccgcg aagggcaccc cgagcaaccc catctccgcg aacgcccccc acaccgtctc
      121 actccacccc gcagccgaat ccacgatctt cgctcgcttg tcgatggaat actcattcgc
      181 cagataccgc ttcaggctat cggccaggag ctgctgctcc tcagtcaaat caaaattcat
      241 ggagtaactc ctggacgaga gaggtcgtca tgcagcaccg cgacgtccgg cgggaccggg
      301 gggtggccct ggggggtcag gctgacccgt tcctgccaag cagaacgatg ccgaccaccg
      361 cccaggttgg gaactcctcc cagggtttcc ggagaacagc gctgcgtggt cagacgaccc
      421 cccagggcca ccccccggcc ccgccgaaca tcgccacgcg caccccgagt acgagccgcc
      481 actacaaccc gagcgtcgcc ttcgcgatga tgttccgctg aatctcgttg gaccccgcgt
      541 agatcgtagc cttgcgcaga ttgcagtacc gcggcgccaa ccgcgccgtg aagtggtcga
      601 agtccatcgc gtcgacgggc cggaacgcct gcgccagcgg ccccgcggcc tgcatcatca
      661 gctcggtgat cgcctgctgg atctcggtgc ccttgatctt gagcatcgac acatcggcgc
      721 ctgggggccg gccgctgcgg cgcatctggt cgaggaagcg caggttggtg atctcgagcg
      781 ccatcagctc gacctcgacc cgcgtgagcc ggtcacggaa gcgcgggtcc tcgatgatcg
      841 ggcggccgtt cttcatctgc tcgctcgcca cttccttcag cttggccagc tcccgcttgg
      901 aggcaccgat tcggccggta cccatgcgct cgtagcccag gaggtacttg gccaccgtcc
      961 agcccttgcc ctcctcgtgc acgaggttct cggccggcac cttgacctcg tcgaagaaga
     1021 cctcgttgac ctccttgctg ccgtcgagca ggacgatcgg ccggaccgtg atgcccaggg
     1081 tcttcatgtc gatcagcagg aacgagatcc cgtcctgccg cttgccgctg gcatccgtgc
     1141 gcaccaggca gaagatccag tccgcgtgct gggccatggt ggtccagatc ttctggccgg
     1201 tgacgacgta gtcaccgttc tgccgcaccg cccgcgtctt gagcgacgcc aggtccgagc
     1261 ccgagcccgg ctccgagtag ccctggcacc agaaatcctc tccctggtag atgcgcggga
     1321 ggaaccgctt cttctgggca tcggtgccga actgcaggag caccggggcg cacatgcgca
     1381 cgccgaaggg cacgaccatc ggagtggctg cgaagccgca ctcctcctcg aagatgtagc
     1441 gctgcaccac gttccagtcg gtcccgcccc actcctgcgg ccagtccggc gccacccagc
     1501 ccttctgggc caggatcttg tgccagcgca ggagctcttc cttggacagc tcctggtagc
     1561 tctccatctt ctggcgcagg tcggcgggca ggttggtgcg gagccagccg cggacttcgt
     1621 cgcggaatgt cagctcctcg ggcgagtagt tcaggtccat ggtcaggctc ctctcagcgg
     1681 agtcgtcgtc ggccccgggc cgggccgagg gccatgctat cccagggcac ggatgaggtc
     1741 cagcagccgg cccagggtgg cgagccgcgc ggccagtccc tcccccgcgg gctcgacccg
     1801 cagggtcgtg atcccggcgc ggcggtaggc ctcgaggcgg gtgcgcacca tcgcctcggt
     1861 gccgagcagg ttcgtcttct ggaccagctc gtcgggcacc cgggccgccg cctcctcgcg
     1921 cttgccggcg agccagaggc gctgcacctc gaccgccacg tcctcgtagc ccgcccggcg
     1981 gaaggcctcg ttgtagaagt tgtgctggcg ggagcccatg gccccgagcg tgaacgcgag
     2041 ccccggcttg cgcggcggga gcagccgctc cacgtcgtcc gagaaggcca cgaagccacc
     2101 ggcctgcagg tcgagctggg cgagcgagcg gccggcccgc gccgcgccgg cggcgaggtg
     2161 gttagtgaag acctgcgcat gatcgggcat gaacgaggtt ccgagccagc cgtcggcgat
     2221 ctcacccgtc atctcaaggc tcttggggga aagggtcgcc agatatatgg gaatcccggg
     2281 ctgcggcctg gccgccgacc ggatcgcttt cccctcgccg cccggcagcg gcagctcgta
     2341 gagcttgccc ttgtacgcca ggtgctcgcc ggtgacggcg cgccggacga tctccacgat
     2401 ctcccgcatg cgctgcaccg gccggtcgaa gcggatgccg tgccagccct cgatgacctg
     2461 cggcccgctc accccgaggc cgaggacgaa gcggccgttg gacatggagg cgaggctcat
     2521 cgcggtcatg gccaccaggg ccggcgtgcg ggtgcccgcc tggatgatcc cggtgcccag
     2581 gcggatgcgc gaggtgcgcg cggccatgaa ggcgagcggc gtgaccgcgt cgtggcccca
     2641 cgcctccgcg gaccagacgt cgtgcacgcc cagccgctcg gcctcggtga cgtaggcgct
     2701 cgcctccgcc cagtcggtct gggcgaggcg gagcgagatc gagacgcgca tcacgcggga
     2761 cgggctgcgc cgagggcggc ggcgatcttc tggagctggc cggcgtgatc caggtcgtgc
     2821 aggcgggtga agagcagggt ctcgcgccag gagagcggcc cgaacatggg atgctcgatg
     2881 cgccgatccg ccgcggtggc ggggtcggcg gcgaggactt cggcgaagag ggcctcgcgc
     2941 tggcggctga gcacctcgta ccacgcctgc gcgctctgga gcggcgcatc ctccaccccg
     3001 cggcgggtcg gccgcggccc cgccgggcgc ccggcgagcg cgtcacggat gccgccgccg
     3061 aagtaaccgt cggccatcac cacgtgggcc atcacctcgt tgccgctcca ctcttcaggc
     3121 gccggtcgct ccgcgaaccg ctcggccggc accgccactg cggccgcgcg taggtcatcc
     3181 atcgccgcgc gcaccttctc gacgagcgca gccggcgtga gcttcgcccc ctgcgcaacc
     3241 agataactcc ggatccgcgc ctgctcctcg tccatcgcgc ctggacttta ccagatgggg
     3301 tcaggtctta cattccgaca ttttttggcg ctctgtgact cactcggttg cctggaaaat
     3361 gtcggaatgt aagacctgac cccgtcatag gccgagatcg gtgaggaagg tgccgatcac
     3421 gcgctggacc tcggcgcggt gggtgcggtt cgggaaatgg gggccgccct cgaccacgtg
     3481 caggcgggcg cccgggatgg tctcggcgac acggcgggac atggccaccg tggggaagct
     3541 gtccaccgag cccgcgatca cctgcaccgg gcagcggatg cggcggcagt ccgggagcca
     3601 ctcggggcgc tggtaggtct cgtcgagcag cgcgagcagc gaggccaccg agtggcggcc
     3661 gagcgcgccg gtcagctccg cgtagcggtc cgggcgcgcc ttcagccgcg ccagcgcctc
     3721 ctcgcccatg cgcagccgca cgttggcgtc gaagtactcg gcgaagcgtc ccgcctccac
     3781 cagtgggcgt tggcgggccc accaggcctt gtcctccctg gtgggctcga agggcggccc
     3841 gctgatcgac accacgcccc gcacccgctc cggggcgtgg accgcgatcc agagcgagag
     3901 cgcgccgccc atcgaggcgc cgccccacac tgcctcgcgg atgccgagcc cgtccagcca
     3961 gtggagcagg tcctcggcct tccgcgcgta ggtgtagtcc tccacccgct cgaagcgcga
     4021 ggagagcccg tggccgcgcg tgtcggggaa caatgcacgc acccggtcga agaagggttc
     4081 caggtccgcg accatgaccg aagagtcctc gaccgatccg ccgtggatcc agaccaccac
     4141 cgggggagcg acgccgggcc ccgcggccag cccggcctcc cgccagtgaa ggcggacgcc
     4201 ggtgggcagg gcgacctcag gcagggccca tcctcttctc caggtagcgc gcgtagcggg
     4261 acatggagta ggtgcagacc cagtagacga ccgcgatgaa gaggtagatg gggaacgggc
     4321 ggatctcgcg gttgttgacg acctgggccg ccttggtcag gtccatgaag ccgatgatgg
     4381 aggcgagcga ggtgtccttg aagagcacga tgaactgggt caccagcgac gggatcatgt
     4441 tcttgagcgc ctggggcagg atcacgaagc gcatgccctg cgatccgctg agcccgagcg
     4501 cggtgcccgc ctcgacctga ccgcgcggca ccgactggat gcccgcgcgg acgatctcgc
     4561 ccaggtaggc ggcctcgaag atcacgaagg cggccagcgc gaccccgaac tccgggatcg
     4621 cggtgtggag cacgatcggc atgatgaacc acatccagaa gatcaccatg accagcggga
     4681 caccccggaa gaactccacg tagagtaccg ccggggtccg gatccaccac tgcggagcga
     4741 gccgggcgat gccgatgaag atgccgagga ggaagcccag gatgatcgac gggatggcca
     4801 gccgcagggt gccgcccacg aagttgccga tgcccaggaa gccctggagc atcaggaacg
     4861 ggaagttgcg ccagatgacg tccaggtcgc ccatggctag cgccgctgcc gcccgcccac
     4921 gatgaggccg gggatcgcgg tgtgccgctc cacccaggac atgatcgtgg cgatcgacac
     4981 gcagaggacc agatacacga gcgtccccgc ggtgagcgcc tcgatggcct tcgcggtgta
     5041 ggtctcgatc tggcgcgtct ggaacgtgag ctcggccacg ctgatggtga gcgccaccga
     5101 cgagttcttg agcaggttga gcgactcgtt ggtgccgggc gggatgatga ggcgcagggc
     5161 caccggcagg atcacgtacc ggtaggcctg gaaggtggtg agcccggagg ccaccgaggc
     5221 ctccagctgc gtgcgcggga tcgactggat gcccgagcgc agcacctcgg agaagcgggc
     5281 cccgctgtag acgccgagag cgcacaccgc ggcccagaac tcgagcccgt gcgcgaagag
     5341 ccattcctgg accgcctggg gcaagagcgg cggcaccgcg aagtaccaga agaacatcca
     5401 gaccagcagc gggacgttgc ggaagaactc cacgtagaag gtcgcggcgg cccgcagcgg
     5461 ccagaaacgc accgtgcgca gcgagcccga gaggacgccg aggacggccg cgagcagcca
     5521 cgccacgatc gacagctcga gcgtggtgat caggccgctc agcagccact ggccggactc
     5581 gccggtccag agcaccgacc agttgaactg atatctcatg tggagctccc cctcagcatg
     5641 acccctcacc ctgcccccct caccccggcc ctctccccag aggggagagg gccggggtga
     5701 gggctacttc gggaccacct gcaggagcat gaagctgcgc acgcgcgcgt ccatcgggta
     5761 cggcagctcg cccttgaccc cgaaccactt ctcgtagatc tcgaagaact tgccggactc
     5821 gatgccgtcc atcagcccgg agttgaccac gttgcgaaag tcggactcgc ccttgcgcat
     5881 cgccatgccg tagggctcgt aggagtagaa atcgcccacg atgtcccact gggccggatt
     5941 cggcgacttg accttgagcc cggcgagctg gatgccgtcg ttggtgtagg cgtccacctg
     6001 gccctgggcc agcgcctgga aggcggcggg ctggtcgggg aactcgcgca ggatggcctt
     6061 gggcgccttc tcgcggatga tccgcgcgtt ggtcgagccc tgctgggccg cgatgcgctt
     6121 gccgtcgatg ttcttgatgc ccttgatggg gctgcc
//
LOCUS       DATMCV010000844         3519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_179243, whole genome shotgun sequence.
ACCESSION   DATMCV010000844 DATMCV010000000
VERSION     DATMCV010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179243"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="VNU03_19450"
     CDS             <1..1823
                     /locus_tag="VNU03_19450"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76706.1"
                     /translation="VIGKDVSLALLQEIADLSEEATRRNLALLQSGEFLYEISLFPSV
                     EYTFRHALTHEVAYSSLLQDRRRALHGRVVTAMEQLYADRLPEHFERLAHHAVRGEVW
                     AKAVAYLRQAGIKAAGRSSNREAVIFLEQAMAALARLPEGRAATEQAIDLRLDLRSPL
                     LQSGQLERVLTLSQEAEAMAEKLGDEQRLARVYTYLINYHYLKGEPDLAIEYGERCLR
                     IGDAAGDVGLQALARGYLGYSCHAQGLYHRAHSILRENVEALELVQGDTPGGQTGVAY
                     VTSSGWLAFTLADLGEFDAAAMSLDRAQRAGEASGHAYTQTIARTMAGLAWLRRGQLE
                     RALPALQRSLDACREKNLDVWRPIPSSLLGLTLVLLGRVEEGMRLLEDGVTLTEELGV
                     RAYLALWTANLGEGLAAARQLDRARTVAQQALDLALRHKERGHQAWALRLMGELSSLA
                     EPPAVGEAEGYYGQALALAEELEMRPLQARTHLGLGRLLRLSGDRERAEDHLATALGL
                     LREMDMRFWLSRAAEELLHMGHLFVVARHNIQLYDYLKREYAGEPVTVILDRRHGQRR
                     ENAEATDSERRRAERRRQIQVDDAIRTRGFAVVPEIIGGA"
     gene            complement(1807..2058)
                     /locus_tag="VNU03_19455"
     CDS             complement(1807..2058)
                     /locus_tag="VNU03_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76707.1"
                     /translation="MIKTGRIEFYDRPTAWGLIVGDDQELYVVRGRDGRGVLPRVGER
                     VSFESGTAVDGKRAAEVRLVSRGLSAFGREAASGIRPLR"
     gene            2215..>3519
                     /locus_tag="VNU03_19460"
     CDS             2215..>3519
                     /locus_tag="VNU03_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP76708.1"
                     /translation="MLDATIAAVEARNPEINAVICRLDDQARAAITAGLPAGPFTGVP
                     YLLKDLGAHYQGVVTSGGGALFKDFVPDHDSEITRRLKRAGLVIVGKTNTPELGLASS
                     TEPRLFGPTRNPWNPAHSAGGSSGGSAAAVAARMVPMAHATDGGGSIRIPASACGLFG
                     LKPTRARNPMGPDQGEGWGGASVGHAVTRSVRDSAALLDATSGPDVGDPYWAPPPARP
                     FLEEVGRDPGRLRIAITTRPWNGQAVDPECEAAARAAARLCESLGHSVEEARPDADAG
                     QLGAATLVIIGANLRAALEARAASLGRELDEADVERITWIRALDGHTARAADYARAIG
                     IMHRTGRQVAPFFSRYDVLLTPTMCRPPYPLGVIDMMTGDPVGYGQAVLSTIAFTSLW
                     NACGNPAMSVPLVWSRAGLPLGVQFVGGFGDEATLFRLAAQLE"
BASE COUNT          481 a         1310 c         1273 g          455 t
ORIGIN      
        1 cggttatcgg caaggacgtg tcgctggcgc tgctgcagga gatcgccgac ctgtcggagg
       61 aggcgacgcg ccgcaacctg gccctgctcc agtcgggcga gttcctgtac gagatcagcc
      121 tcttcccgag cgtggagtac acgttccgcc acgccctgac ccacgaggtg gcctactcga
      181 gcctgctcca ggatcggcgc cgcgcgctcc acgggcgcgt ggtgaccgcc atggagcagc
      241 tctacgccga ccgcctgccc gagcatttcg agcggctggc ccaccacgcg gtgcgcggcg
      301 aggtgtgggc gaaggcggtg gcgtacctgc gccaggccgg catcaaggcg gcgggacgct
      361 cctccaaccg ggaggcggtg atcttcctcg agcaggccat ggccgcgctc gcccggctgc
      421 ccgagggccg cgcggcgacc gagcaggcca tcgacctgcg cctggacctg cgctcgccgc
      481 tgctccagtc gggacagctc gagcgggtgc tgacgctctc gcaggaagcg gaggcgatgg
      541 ccgagaagct cggcgacgag cagcggctgg cgcgcgtcta cacctacctg atcaactacc
      601 actatctgaa gggcgagccc gacctcgcca tcgagtatgg ggagcgctgc ttgcgcatcg
      661 gcgacgcggc cggcgacgtg ggcctgcagg cgctcgcgcg cggttacctg ggctacagct
      721 gtcacgcgca gggcctctat caccgcgcgc actcgatcct tcgcgagaac gtggaagccc
      781 tcgagctggt ccagggcgac actcccggtg gccagaccgg cgtcgcctac gtcacctcga
      841 gcggctggct cgccttcacc ctggccgacc tcggcgagtt cgacgccgcc gccatgtcgc
      901 tcgaccgggc gcagcgggcg ggcgaggcga gcgggcacgc ctacacccag accatcgcgc
      961 ggaccatggc tgggctcgcc tggctgcggc gcggccagct cgagcgggcg ctccccgcgc
     1021 tgcagcggag cctcgacgcc tgccgcgaga agaacctcga cgtgtggcgg ccgattccgt
     1081 cctccctgct cgggctcacg ctcgtgctgc tcgggcgggt cgaggaaggc atgcggctcc
     1141 tggaggacgg cgtcaccctc accgaggagc tgggcgtgcg cgcctacctc gcgctctgga
     1201 ccgcgaacct gggcgagggc ctggccgccg cgcggcagct cgaccgcgcg cgcaccgtgg
     1261 cgcagcaggc gctcgacctg gcgctccggc acaaggagcg cgggcaccag gcgtgggcgc
     1321 tgcgcctgat gggcgagctg tcgtcgctgg ccgagccccc cgcggtcggc gaggccgagg
     1381 ggtactacgg ccaggcgctc gccctggccg aggagctgga gatgcgcccg ctgcaggccc
     1441 gcacccacct gggactgggc cggctgctgc gcctgtccgg cgatcgggaa cgggccgagg
     1501 atcacctggc gaccgccctc ggcctgttgc gcgagatgga catgcgattc tggctctcgc
     1561 gggccgccga ggagctcctg cacatgggcc atctcttcgt ggtcgcgcgc cacaacatcc
     1621 agctctacga ctatctgaag cgcgagtacg cgggggagcc ggtcaccgtc atcctggacc
     1681 ggcggcacgg gcagcggcgg gagaacgcgg aggccaccga ctccgagcgc cggcgggccg
     1741 agcgccgccg ccagatccag gtcgacgacg ccatccgcac gcggggcttc gcggtcgtgc
     1801 ccgagatcat cggaggggcc tgatcccgct cgccgcctcc cggccgaagg ccgagagccc
     1861 gcggctcacc agccgcacct cggccgcgcg cttgccgtcc accgcggtgc cggactcgaa
     1921 cgacacgcgc tcgcccactc gcggcagcac tccgcgcccg tcgcgtcccc ggacgacgta
     1981 gagctcctgg tcgtctccca cgatgagacc ccaggccgtc ggccggtcgt agaactcgat
     2041 gcgtcccgtc ttgatcaccg cgcccgcatg ctacggcgtc gcgcgagcgc gccgcaatgt
     2101 ccagaatgtc ggacacgccg cccgggagga acgatgaaga ccgacgagtt cgcgcgccac
     2161 gatgggacgg cactggcgga actgatacgg cgaaaggagg tcagcgcgag cgaggtgctc
     2221 gacgccacga tcgccgccgt ggaggcccgt aaccccgaga tcaacgcggt gatctgccgg
     2281 ctcgacgacc aggcgcgggc cgcgatcacg gccgggctgc ccgccgggcc cttcaccggg
     2341 gtgccctatc tgctgaagga cctgggcgcg cactaccagg gcgtggtcac cagcggcggc
     2401 ggcgcgctct tcaaggactt cgtgccggat cacgacagcg agatcacccg gcgcctcaag
     2461 cgagccggcc tcgtgatcgt cggcaagacc aacacccccg agctcggcct ggccagctcc
     2521 accgagccgc gcctgttcgg gcccacccgc aacccgtgga atcccgcgca cagcgcgggc
     2581 ggctcgagcg gcggctccgc ggcggcggtg gcggcgcgca tggtgccgat ggcgcacgcc
     2641 accgacggcg gcggctcgat ccgcatcccc gcgtccgcct gcggcctctt cgggctcaag
     2701 cccacccgcg cccgcaaccc gatgggcccc gatcagggcg agggctgggg cggcgcctcg
     2761 gtgggccacg cggtgacccg cagcgtgcgg gacagcgcgg cgctgctcga cgccacctcc
     2821 gggcccgacg tgggcgatcc ctactgggcg ccgccgcccg cgcgcccgtt cctggaggag
     2881 gtgggtcgcg atccgggccg gctgcgcatc gccatcacca cgcggccctg gaacggccag
     2941 gcggtggatc cggagtgcga ggcggccgcc cgcgcggcgg cccggctctg cgagagcctc
     3001 gggcactcgg tggaggaggc gcgcccggac gcggatgccg gtcagctcgg agccgcgacg
     3061 ctcgtgatca tcggcgcgaa tctgcgagcc gccctggaag cgcgcgcggc ctcgctcggc
     3121 cgcgagctcg acgaggccga cgtggagcgg atcacgtgga tccgcgcgct ggacggccac
     3181 accgcccgcg cggccgacta cgcccgggcc atcgggatca tgcaccgcac cgggcgccag
     3241 gtcgcgccgt tcttctcgcg ctacgacgtg ctgctgacgc cgaccatgtg ccgcccgccg
     3301 tatccgctcg gggtcatcga catgatgacc ggcgacccgg tgggctacgg gcaggcggtg
     3361 ctctccacca tcgcgttcac gtcgctctgg aacgcgtgcg gcaacccggc gatgtcggtg
     3421 ccgctcgtct ggtcgcgagc cgggctgccg ctcggcgtgc agttcgtggg cggcttcggc
     3481 gacgaggcga cgctcttccg cctggccgcc cagctcgag
//
LOCUS       DATMCV010000845         8547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_450020, whole genome shotgun sequence.
ACCESSION   DATMCV010000845 DATMCV010000000
VERSION     DATMCV010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450020"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1189)
                     /locus_tag="VNU03_19465"
     CDS             complement(182..1189)
                     /locus_tag="VNU03_19465"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP76709.1"
                     /translation="MKFDVGILASQPLPVLVRQVQLAESLGFDTAWIADTHLVCRELW
                     VTLAACAAGTSRIRLGPGIAVPHTRHISVTASAIATLHELAEGRVVIGIGTGGSAAET
                     MGLSVGKVARIATLESMAAALRRLLGRESIRLDNGTDARLAWLERAPAIPLYLAGSGP
                     RMLDAAGRLGDGAIMYSGVAPWLVEAALGCVKAGARSAGRTPEDLDVAIWAPTSIAPD
                     RALARDHVRGRVASALRHAFPVAWSPEDQKVIAQVRAAYDSYQHASAASKHRLLVTDR
                     FVDLMALAGRPEEVREQVRGIIGVGGVGRIILLPQVPGQDFVEREEVLRLFAERVMAR
                     L"
     gene            complement(1200..1973)
                     /locus_tag="VNU03_19470"
     CDS             complement(1200..1973)
                     /locus_tag="VNU03_19470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP76710.1"
                     /translation="MPTIEHKGVKLAYEEKGSGSPTFLFIHGWTCDRSFFKAQADHFA
                     KRHRTVSVDLRGHGESDKPAGAYPISAYVDDLAHVIDTLKLGKVVAVGHSMGGITVLE
                     LGARHADKVAAIVMVDPAPLVWPAELTGPVGDVVSAIEAGNQEPRRQFIANALFLPTS
                     DKKLMDSVLKTMMAAPAPVAAAAMKGILAFDGPGMAKRCTVPALHLAATPPLNAPHMM
                     SQLMPKVVNGWTVGAGHFNMMETPDQVNSMIEGFLRHHV"
     gene            2071..3387
                     /locus_tag="VNU03_19475"
     CDS             2071..3387
                     /locus_tag="VNU03_19475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP76711.1"
                     /translation="MANTSRFDVAVIGGGPGGSSVATALARRGRSVLLLERDRFPRFH
                     IGESQLPWINGVLDALGAHDAVAKAGFVEKWGASFAEIDGTPAAYVDFTAAVETPVPQ
                     TYQVPRAAFDEVLLRHAERSGVIVREEHRALDAAFDPDGVTLRFTGPAGPGEARVQAV
                     VDASGRSGLLARRIGGYETDVMLQHVAVHAQYEGVPRASGRPAGDIRVLTRADVGWVW
                     LIPISERVTSVGAVVPQALHRRESKATAEESLEHYLRETPAAPALLREATRVSPARFD
                     ADYSYLARAMAGDRWVAVGDAAAFLDPIFSTGVLLAMQGGLEAAEAIDAGLRAGDLSA
                     RRFEGYERVVRRRYHYFRRFVVGFYDPYFRRLLFRRSRRLGIYEAVLSALAGNWRPTL
                     GTRLRIQLFFAIVAVKRFFRVAPPGDATWRPEAAGLTSGAEPTPRR"
     gene            complement(3353..4258)
                     /locus_tag="VNU03_19480"
     CDS             complement(3353..4258)
                     /locus_tag="VNU03_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWP76712.1"
                     /translation="MLSPSDVTFYREQGYLVVPDVLDASTLDTVRRELARILDGARAV
                     TAHTDVYDLEPGHRADDPRVRRVKTPHRFFPVFDRLMRHPRLVAILTALLGDAIRLHG
                     SKINLKSPRYGSPVEWHQDWAFYPHTNDELLAVGVMLDDCTSDNGPLLVVPGTHRGPT
                     FDHHTGDHPGGRYFCGAIDPAAIRDEIARAVPLTGRAGSMSFHHVRLVHGSAQNVSSL
                     PRTLLLYEYGAADAWPLMGVPSLADFDGRLITGEPGLVPRLVPAPVRMPLPPALRQGS
                     IYENQSDAARRYFPVTDEASAPPRT"
     gene            4363..5715
                     /locus_tag="VNU03_19485"
     CDS             4363..5715
                     /locus_tag="VNU03_19485"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76713.1"
                     /translation="MLDSRISRRSLLAGAVGTSALLTLPRPAFPAVKKGTSLRLWILK
                     TYVEPTNKAIEASAQRWAQKNGASVTVEYFTFEDMQTKYVAAIESNSTPDIGQLETSA
                     PARFAGMGQLIDLTTFARQIEGQVGKAPANVTAVTMVGDKVYSLPWYVMGAFWYAWRD
                     VFEKAKVKLPTSFEEAKQAAAALNRPKDNFYGMGQSWNRTADGYGVMQSLMYSHGVSW
                     AGKDGKYKSIKTAAMQNVMKWATDIYKDGVQPADTLTWTGSGNNENFIAKNIAQTSNG
                     PSITFALENALAKATDAKDRKAKEEALANHVALPHPAGPDGRRMWAIAMSFGIFKTSK
                     DPEAAMSLIAHLLSPEETVAVMKDSYGQFGPVLDKARAASKDYFNKNDNYRTFGRAAE
                     WFAPTGWPGPTTAAAAEVQASNVLTDAPAKVIVDKWSVDQAIEWEDKKIKEIYDTLRG
                     "
     gene            5717..6655
                     /locus_tag="VNU03_19490"
     CDS             5717..6655
                     /locus_tag="VNU03_19490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWP76714.1"
                     /translation="MALPHTVGIPTVRARASLWRRARTRETTTALLFLLPLFVLVVAL
                     IVYPVYRAVWLSLTDKLVGYPERFVGLRNYAYLAQDDTFHAVIRNSLVFTVGSVGLKV
                     VTGVLMALVLNNVIRGRNFFRGLFLLPWITSTVIIALTWRWMFDAFPGRGFFNSVLLD
                     AGVMRRPIAFMATPEGAMMAVIVANWWRGFPFFGVSFLAGLASIPRELYEAASVDGAS
                     SWRRFWHITLPGLKHVIIVTTLLSFILTINDFNIVYVMTRGGPGTATQIFATYSYQVA
                     FGQLRWGRGVTICLFVVPILVLGIIMISRYLLRDRK"
     gene            6662..7480
                     /locus_tag="VNU03_19495"
     CDS             6662..7480
                     /locus_tag="VNU03_19495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWP76715.1"
                     /translation="MTTKALTWLGMAVFLLVILVPFWWIASMSFKTYEQIQFATSIYV
                     PNPFTWENYTGLWTETRFPLWLRNSLVTALVVTATTTVVASLGGYAVARLRFPGRESV
                     ASLILILYLIPPALLFIPLYRVLAELGATNHLSSLFLSYPTFTVPFCTWLLIGFFKAL
                     PDELEEAALIDGAGRVTAFWRVLLPLAAPGLVASAIFAFTLSWNEFLYALVFIQDEQA
                     ITVPVGLNLLIYGDVFHWGQLMAASVITTIPVVALYVFIHRWMVEGLVAGSVKG"
     gene            7503..8417
                     /locus_tag="VNU03_19500"
     CDS             7503..8417
                     /locus_tag="VNU03_19500"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP76716.1"
                     /translation="MATIAAPVTISPTHPAFAAEVSGVDLTRRLDDATFERIAVAFDD
                     YSVLVFRGQALSDEQQMAFSERWGPLETTVRTLGGEDRLGAHIVDLSNTDPDGKLMGW
                     DDRRMLYQSGNQLWHSDSSFKPVPAHSSALSARVIPPEGGETEFASMRVAYEHLDENV
                     RRDLDRLIVVHSFGFSRSMIDPGIGTEIGRDYPPVRHALVRANPRNGRRSLYIGSHAW
                     FIEGIGLDESRILIARLLEQTTRPDRVYRHRWQVGDLVMWDNRCVLHRGRPWDSARYK
                     RVMHRTTVAGDGATADPAFLHELTATRA"
BASE COUNT         1252 a         3032 c         2964 g         1299 t
ORIGIN      
        1 gtgtgcaccg aggagggcgc ggtcgatccg ggcgccacct ggctcaccga gttctgggcc
       61 ggcggccgca gccgcgggtg gatcgtggcc cagacctcgc tgctccacgt ctacggccac
      121 tacttcgagg ccctcgcgac caagggcctc tggggcgcgc gcagccgcta ggtctggagc
      181 gctagagccg ggccatcacc cgctcggcga agaggcgcag cacttcctcg cgctccacga
      241 agtcctgccc cggcacctgc ggcaacagga tgatccgccc gacgccgccc acgccgatga
      301 tccctcgcac ctgctcgcgt acctcctcgg gtcgtccggc cagcgccatg aggtccacga
      361 agcggtcggt cacgagcagg cggtgcttcg acgcggcgct cgcgtgctgg taggagtcgt
      421 aggccgcgcg gacctgggcg atcaccttct gatcctcagg gctccacgcg accggaaacg
      481 cgtggcggag cgcggaggcc acgcggccgc ggacatgatc ccgcgcgagg gcgcggtcgg
      541 gcgcgatcga ggtaggcgcc cagatcgcca cgtccagatc ttccggcgtg cgtccggccg
      601 atcgcgcgcc ggccttcacg cagccgagcg cggcctcgac cagccacggc gcgaccccgg
      661 agtacatgat cgcgccgtcg cccaggcgcc cggccgcgtc cagcatgcgg ggcccggagc
      721 cggccagata gagcgggatg gccggcgcgc gctcgagcca cgccaggcgc gcgtcggtgc
      781 cgttgtcgag gcggatcgac tcgcggccga ggagtcgccg cagcgccgcc gccatggact
      841 cgagggtggc gatccgcgcg accttcccga ccgagagccc catggtctcg gccgcgctcc
      901 cgccggtgcc gatgccgatg accacgcggc cttcggccag ctcgtggagc gtggcgatcg
      961 cgctcgcggt caccgagatg tggcgggtgt gcggcaccgc gatgccgggg cccaggcgga
     1021 tgcgcgaggt cccggccgcg cacgcggcga gggtgaccca cagctcgcgg cagaccaggt
     1081 gggtgtcggc gatccacgcg gtgtcgaacc cgagcgactc cgcgagctgc acctggcgaa
     1141 ccagcaccgg gaggggctgg ctcgccagga tgccgacgtc gaacttcacg aagatcggct
     1201 cagacgtggt gccgcaggaa gccctcgatc atcgagttca cctggtccgg agtctccatc
     1261 atgttgaagt gcccggcgcc gaccgtccag ccgttcacca ccttcggcat caactgcgac
     1321 atcatgtgcg gcgcgttgag cggcggcgtg gcggccaggt gcagggccgg caccgtgcag
     1381 cgcttggcca tgcccggccc gtcgaaggcc aggatgccct tcatcgcggc ggcggcgacc
     1441 ggcgcgggcg ccgccatcat ggtcttgagg accgagtcca tcagcttctt gtcggaggtg
     1501 ggcaggaaga gcgcgttggc gatgaactgg cgccgcggct cctggttgcc cgcctcgatc
     1561 gcgctcacca cgtcgcccac cgggcccgtg agctcggcgg gccagacgag cggcgcgggg
     1621 tccaccatca caatcgccgc caccttgtcc gcgtggcgcg cgcccagctc gagcacggtg
     1681 atgccgccca tgctgtggcc gaccgcgacc accttgccga gcttcaaggt gtcgatcacg
     1741 tgcgcgagat cgtccacgta ggccgagatc ggataggccc cggcgggctt gtcgctctcc
     1801 ccgtggccgc ggaggtcgac cgacaccgtg cgatgacgct tcgcgaaatg gtcggcctgg
     1861 gccttgaaga acgagcggtc gcaggtccag ccgtggatga agaggaaggt ggggctgccg
     1921 gagcccttct cctcgtacgc cagcttcacg cccttgtgct cgatggtcgg catggggtct
     1981 cctccttgaa gggtgccgcg acgtttatcg gacgaggccg agaatcagcc cgaaccccgg
     2041 ccccgtcaag gggtatcctc tatagccgag atggcgaaca cgagccggtt cgacgtggcg
     2101 gtgatcggcg ggggccccgg cggctcgagc gtggccaccg cgctcgcccg acgcggtcgg
     2161 agcgtgctgt tgctcgagcg cgatcgcttc ccccgcttcc acatcggcga gtcgcagctg
     2221 ccgtggatca acggggtgct ggacgccctc ggagcccacg acgcggtggc caaggccggc
     2281 ttcgtggaga agtggggcgc gagcttcgcg gagatcgacg ggacgcccgc cgcctacgtg
     2341 gatttcaccg cggcggtcga gaccccggtc ccgcagacct accaggtgcc gcgggccgcg
     2401 ttcgacgagg tgctgctccg ccacgccgag cgctccgggg tcatcgtgcg cgaggagcac
     2461 cgtgcgctcg acgcggcgtt cgacccggac ggggtcaccc tgcgcttcac cggccccgcc
     2521 gggccgggtg aggcccgcgt gcaggcggtg gtcgacgcct ccggtcgctc cggcctgctg
     2581 gcccgtcgga tcggcggcta cgagaccgac gtcatgctgc agcacgtcgc ggtgcacgcc
     2641 cagtacgagg gcgtcccgcg ggcctccggc cgcccggcgg gcgacatccg cgtgctcacg
     2701 cgcgcggacg tggggtgggt ctggctgatc ccgatctcgg agcgcgtcac cagcgtgggg
     2761 gccgtcgtgc cgcaggcgct ccaccggcgc gagtcgaagg cgaccgcgga ggagagcctc
     2821 gaacactacc tgcgcgagac gccggccgcg cccgcgctgc tgcgcgaggc cacgcgcgtg
     2881 tcccccgcgc gcttcgacgc cgactactcc tacctcgcgc gcgcgatggc gggggaccgg
     2941 tgggtcgcgg tgggcgacgc ggccgcgttc ctcgatccga tcttctccac cggggtgctg
     3001 ctcgccatgc agggcgggct cgaggcggcg gaggcgatcg acgcgggcct gcgggcgggc
     3061 gatctctccg cgcggcgctt cgagggctac gagcgggtgg tgcggcggcg ctaccactac
     3121 ttccgccgct tcgtggtggg gttctacgat ccctacttcc gccgcctgct gttccggcgc
     3181 tcgcgccggc tcggcatcta cgaggcggtg ctctctgcgc tggccggcaa ctggcgcccg
     3241 accctcggca cccggctgcg catccagctc ttcttcgcga tcgtcgcggt gaagcgcttc
     3301 ttccgcgtgg ccccgccggg cgacgcgacc tggcggcccg aggccgccgg cctcacgtcc
     3361 ggggcggagc cgacgcctcg tcggtgaccg gaaagtagcg gcgcgccgcg tccgactggt
     3421 tctcgtagat cgacccctga cggagcgcgg ggggcagggg catgcgcacc ggcgccggca
     3481 ccagccgcgg aacgagaccg ggctcgccgg tgatcagccg gccgtcgaag tcggcgaggc
     3541 tcggcactcc catgagcggc cacgcgtcgg cggcgccgta ttcgtagagc aggagcgtgc
     3601 gcggcaggct cgagacgttc tgggccgagc cgtgcacgag gcgcacgtgg tggaagctca
     3661 tggagccggc gcgcccggtc agcggcaccg cgcgcgcgat ctcgtcgcgg atcgcggcgg
     3721 gatcgatggc cccgcagaag tagcgtccgc cggggtgatc gcccgtgtgg tgatcaaatg
     3781 tcggcccgcg atgcgtgccc ggaaccacca ggagcgggcc gttgtcgctc gtgcagtcgt
     3841 cgagcatcac cccgaccgcg aggagctcgt cgttggtgtg cgggtagaac gcccaatcct
     3901 ggtgccactc caccggcgag ccgtagcgcg gcgatttgag attgatcttg gagccgtgca
     3961 gccggatcgc gtcgccgagg agcgcggtca ggatcgccac cagccgcggg tggcgcatca
     4021 ggcggtcgaa caccgggaag aaccggtggg gcgtcttcac ccggcgcacc cgcgggtcgt
     4081 ccgcgcgatg gcccggctcg agatcgtaga cgtcggtgtg cgcggtcacc gcgcgcgcgc
     4141 cgtcgaggat gcgcgcgagc tcccgccgca cggtatcgag cgtcgaggcg tcgaggacgt
     4201 cgggaaccac caggtagccc tgctcgcgat agaaggtcac gtcggaaggg gagagcatga
     4261 gagaagcgta gcacgggaca cgcggcgctt gacagggcgc cgccggtgac cgacattcgg
     4321 ggcgcgtcgt cggggtcaaa gacgatccaa gggggacacg ccatgctcga ttcgcgcatc
     4381 agccgtcgct cactcctcgc cggggccgtc gggacatccg ccctcctgac cctgccgcgg
     4441 ccggccttcc cggccgtcaa gaagggcacc agcctccgcc tgtggatcct gaagacctac
     4501 gtggagccga ccaacaaggc gatcgaggcg agcgcccagc ggtgggcgca gaagaacgga
     4561 gcgagcgtca ccgtcgagta cttcacgttc gaggacatgc agaccaagta cgtggccgcc
     4621 atcgagagca acagcacccc cgacatcggc cagctcgaga ccagcgcccc cgcgcgcttc
     4681 gcgggcatgg gccagctcat cgacctcacc accttcgccc gccagatcga ggggcaggtg
     4741 ggcaaggcgc ccgccaacgt gaccgcggtc accatggtcg gcgacaaggt ctactcgctg
     4801 ccgtggtacg tgatgggcgc cttctggtac gcgtggcgcg acgtgttcga gaaggcgaag
     4861 gtgaagctgc ccaccagctt cgaggaagcc aagcaggccg ccgctgcgct caaccggccc
     4921 aaggacaact tctacggaat gggccagagc tggaaccgca ccgccgacgg ctacggggtc
     4981 atgcagagcc tgatgtactc ccacggggtg agctgggccg gcaaggacgg caagtacaag
     5041 tcgatcaaga cggccgcgat gcagaacgtg atgaagtggg cgaccgacat ctacaaggac
     5101 ggggtgcagc cggccgacac gctcacgtgg accggctccg gcaacaacga gaacttcatc
     5161 gccaagaaca tcgcccagac ctccaacggc cccagcatca ccttcgcgct cgagaacgcg
     5221 ctcgccaagg ccaccgacgc caaggaccgg aaggcgaagg aggaggcgct cgccaaccac
     5281 gtggccctgc cgcacccggc cggaccggac ggccgccgca tgtgggccat cgcgatgagc
     5341 ttcgggatct tcaagaccag caaggacccg gaggcggcca tgagcctgat cgcccacctg
     5401 ctgtctcccg aggagacggt ggcggtcatg aaggactcct acgggcagtt cgggccggtg
     5461 ctcgacaagg cccgcgccgc gtcgaaggac tacttcaaca agaacgataa ctaccggacc
     5521 ttcggccggg ccgcggagtg gttcgccccg accgggtggc ccggccccac caccgcggcg
     5581 gccgccgagg tgcaggcgag caacgtgctc accgacgcgc cggccaaggt catcgtggac
     5641 aagtggtcgg tcgatcaggc catcgagtgg gaggacaaga agatcaagga aatctacgac
     5701 accctgcgcg ggtgacgtgg cgctccccca caccgtcggc atcccgaccg tgcgggcgcg
     5761 cgcctccctc tggcggcgcg cccgcacccg ggagaccacg acggcgctcc tgttcctgct
     5821 gccgctgttc gtcctggtgg tggccctcat cgtctacccg gtctaccggg cggtctggct
     5881 ctcgctcacc gacaagctgg tgggctatcc cgagcggttc gtgggcctgc gcaactacgc
     5941 gtacctggcg caggacgaca ccttccacgc ggtcatccgc aacagcctcg tcttcacggt
     6001 gggctcggtg ggcctcaagg tggtcaccgg ggtcctcatg gcgctggtgc tcaacaacgt
     6061 gatccgcggc cgcaacttct tccgcgggct cttcctgctg ccctggatca cctcgaccgt
     6121 gatcatcgcg ctcacctggc ggtggatgtt cgacgccttc cccggccgcg ggttcttcaa
     6181 cagcgtgctg ctcgacgcgg gggtgatgcg ccgcccgatc gcgttcatgg ccaccccgga
     6241 gggggccatg atggcggtga tcgtggcgaa ctggtggcgc ggcttcccgt tcttcggggt
     6301 gtcgttcctg gccgggctcg cgtccatccc ccgcgagctc tacgaggcgg cctcggtgga
     6361 cggcgcctcc tcctggcgcc gcttctggca catcacgctg cccgggctca agcacgtgat
     6421 catcgtcacc accctgctga gcttcatcct gaccatcaac gacttcaaca tcgtctacgt
     6481 gatgacccgg ggcgggcccg gcaccgcgac ccagatcttc gcgacctatt cctatcaggt
     6541 ggcgttcggc cagctccgct ggggccgcgg ggtgacgatc tgtctcttcg tggtgcccat
     6601 cctggtgctc ggcatcatca tgatcagtcg ctacctcctc cgcgaccgca agtaacccgc
     6661 catgaccacg aaagccctca cgtggctcgg catggccgta ttcctcctcg tgatcctggt
     6721 gccgttctgg tggatcgcga gcatgtcgtt caagacctac gagcagatcc agttcgccac
     6781 cagcatctac gtgccgaacc cgttcacctg ggagaactac accgggctct ggaccgagac
     6841 ccgcttcccg ctctggctgc gtaacagcct ggtgaccgcg ctggtggtca ccgccaccac
     6901 cacggtggtg gcgagcctcg gcggctacgc ggtggcgcgc ctgcgcttcc ccgggcgcga
     6961 gtcggtggcg agcctgatcc tgatcctcta cctgatcccg cccgcgctgc tgttcatccc
     7021 gctctaccgg gtgctggccg agctgggcgc caccaatcat ctgtcgtcgc tcttcctctc
     7081 ctatccgacc ttcacggtgc ccttctgcac ctggctcctc atcggcttct tcaaggcgct
     7141 gcccgacgag ctggaggagg cggccctcat cgacggggcc ggccgggtca ccgcgttctg
     7201 gcgggtgctg ctgccgctgg ccgccccggg gctggtggcc tctgccatct tcgcgttcac
     7261 gctctcgtgg aacgagttcc tgtatgcgct ggtcttcatc caggacgagc aggccatcac
     7321 ggtgccggtc ggcctcaacc tgctcatcta cggtgacgtc ttccactggg gccagctcat
     7381 ggccgcctcg gtgatcacca cgatcccggt ggtcgccctc tacgtcttca tccatcggtg
     7441 gatggtggag gggctggtcg ccgggtcggt gaagggctag aatttcccgc caggaggtct
     7501 ccatggcgac catcgcagct cccgtcacca tcagcccgac ccatcccgcg ttcgcggccg
     7561 aggtctccgg cgtggacctc acccgccggc tcgacgacgc caccttcgag cgcatcgcgg
     7621 tggccttcga cgactactcg gtcctggtct tccgcggcca ggccctgagc gacgagcagc
     7681 agatggcctt cagcgagcgc tgggggccgc tcgagaccac cgtgcgcacc ctcggcgggg
     7741 aggatcggct gggggcccac atcgtcgacc tctccaacac cgacccggac ggcaagctga
     7801 tgggctggga cgatcggcgc atgctctacc agtccggcaa ccagctctgg cacagcgaca
     7861 gctcgttcaa gccggtgccc gcgcactcct cggccctctc cgcgcgggtg atcccgccgg
     7921 aggggggcga gaccgagttc gcgagcatgc gcgtcgcgta cgagcatctt gacgaaaacg
     7981 tacgccgcga cttagatcgg ctaatagtcg tgcacagctt cggcttctcg cgcagcatga
     8041 tcgaccccgg catcggcacc gagattggcc gcgactaccc gccggtgcgc cacgcgctgg
     8101 tgcgggccaa cccgcgcaac ggccgccggt cgctctacat cggctcgcac gcctggttca
     8161 tcgaggggat cgggctcgac gagagccgca tcctgatcgc gcggctgctc gagcagacga
     8221 cgcggcccga tcgcgtgtac cggcaccgct ggcaggtggg agatttggtg atgtgggaca
     8281 accggtgtgt gctgcaccgc gggcgcccgt gggacagcgc gcgctacaag cgggtcatgc
     8341 atcggaccac ggtggcgggc gatggggcga ccgccgatcc cgccttcctc cacgagctga
     8401 cggcaacccg ggcctgatcc ctccggcccg ggtcgccgcc gtgcggtctc gctagccggc
     8461 gctgggccgg ctctcgtccg tcgccgcggg ctggtggctc ccatcctcgg gcgccatgcc
     8521 gcgcgtcccc gagccctgcg gcccgcg
//
LOCUS       DATMCV010000846         2389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_179339, whole genome shotgun sequence.
ACCESSION   DATMCV010000846 DATMCV010000000
VERSION     DATMCV010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179339"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VNU03_19505"
     CDS             complement(<1..242)
                     /locus_tag="VNU03_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWP76717.1"
                     /translation="MRPDYLKLARDQFDRGELSPAALKQVEDRAVDEAVRLQEEAGLD
                     VITDGELRRYAFFGHLIDALDGFDKLGGWAIPFHNEA"
     gene            complement(415..1638)
                     /locus_tag="VNU03_19510"
     CDS             complement(415..1638)
                     /locus_tag="VNU03_19510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP76718.1"
                     /translation="MAVDSKPVGGSGGGAAVAPPQSQKALLELVDRLTREHIAPRAAE
                     YDAAGANPIDSWRVLGRDGFLASCIPTAYGGLGLDMPTYVGVIRTIARGCANTAMTVH
                     MHSTVMRFIDALGTDAQKRCYFAEVVEHGRLFGSWGSEPAVSLSRSFLMETVVRQDRD
                     DYVVDGVKHFCTMALGASHYMVWCALEGGTDMGKALLQVLVPADAPGIATDGKWNTLG
                     MRATFSPSVTFTAVRVPRDAALGDPGTALRVGVVESFALGYAAVYVGIAEGALAFAID
                     YAKQRIVKPENVPVAHDPTVQRHVGELAAHLDAARLVLADTAERWEAADVQERGVLGN
                     KAKYIATEVGLAVTSKVIQVVGGRGAYKDFPAERAFRDLRTSTLMPPTVDRMLEGIGK
                     NALGIDGAMFKVAGS"
     gene            complement(1645..2100)
                     /locus_tag="VNU03_19515"
     CDS             complement(1645..2100)
                     /locus_tag="VNU03_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019747088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP76719.1"
                     /translation="MAVKVLELHHHGIRVGPSPAEVKKAYGFYHDVLGLDADQGRPVI
                     PTIDGFWMDVGGTAQIHLMGVDGQSKFAQGPDKDPSRPHVALAVPDIQEAKAELDRMG
                     VEHWVTRGVVGPESQQIFMYDPFGNMIELHQAGTCRCITGNRQPVKGMS"
     gene            complement(2081..>2389)
                     /locus_tag="VNU03_19520"
     CDS             complement(2081..>2389)
                     /locus_tag="VNU03_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76720.1"
                     /translation="FSACFAGLAGSLDALRLTVVPVESLYWTTSGQVVMMTLLGGAGT
                     FFGPFVGAATFLVLEERLSLVLESWPLVIGTIFSAFVLFLPKGIWGTLTGGIHGRQSA
                     "
BASE COUNT          358 a          813 c          837 g          381 t
ORIGIN      
        1 gcctcgttgt ggaaggggat ggcccagccg ccgagcttgt cgaagccgtc gagggcgtcg
       61 atcaggtggc cgaagaaggc gtagcggcgc agctcgccgt cggtgatcac gtcgaggccg
      121 gcttcttcct ggagccgcac cgcctcgtcg accgcgcggt cctccacctg cttgagcgcg
      181 gcgggggaga gctcgccgcg gtcgaactgg tcgcgggcga gcttgagata gtccggtcgc
      241 aacagacttc ctatcacgtc gctgcggatc gcttgggcgg gcatccctgg cttctcttta
      301 tatacgttcg gctcgcctat ggctcgcctc acctgtgtaa cccggctcgc cttcggctgc
      361 gccttgcgcc gtgattcgat cgcctgcagc tatggcctgc tccgcgattc cgcgctagct
      421 gccggcgacc ttgaacatgg cgccgtcgat gccgagcgcg ttcttgccga tgccctcgag
      481 catccggtcc acggtgggcg gcatgagggt ggaggtgcgc aggtcgcgga aggcgcgctc
      541 ggctgggaag tccttgtagg cgccgcggcc gcccaccacc tggatgacct tggaggtgac
      601 cgcgaggccg acctcggtgg cgatgtactt ggccttgttg cccagcacgc cgcgctcctg
      661 cacgtcggcg gcctcccagc gctccgcggt gtcggccagc acgagccggg ccgcatcgag
      721 gtgtgcggcc agctccccga cgtgccgctg cacggtgggg tcgtgggcca ccgggacgtt
      781 ctcgggcttc acgatgcgct gcttggcgta gtcgatcgcg aaggcgagcg caccctccgc
      841 gatgcccacg tacaccgcgg cgtagccgag ggcgaagctc tccaccacac ctacccgcag
      901 cgcggtgccg gggtcgccga gcgccgcgtc cctcgggacg cgcaccgcgg tgaaagttac
      961 cgacgggctg aaggtcgccc gcatgccgag ggtgttccac ttgccgtcgg tggcgatgcc
     1021 gggagcgtcg gccggcacca gcacctggag cagggccttg cccatgtcgg tgccgccctc
     1081 gagcgcgcac cagaccatgt agtgcgaggc gcccagggcc atggtgcaga agtgcttcac
     1141 cccgtcgacg acgtaatcgt cgcggtcctg gcgcaccacc gtctccatca ggaagctgcg
     1201 cgagaggctc accgcgggct cgctgcccca gctgccgaag aggcggccgt gctcgacgac
     1261 ctcggcgaag tagcagcgct tctgcgcgtc ggtgccgagg gcgtcgatga agcgcatgac
     1321 cgtggagtgc atgtgcacgg tcatggcggt gttggcgcag ccgcgggcga tggtgcggat
     1381 caccccgacg taggtcggca tgtcgagccc gagcccgccg tacgcggtgg ggatgcacga
     1441 ggccaggaag ccgtcacgac cgagcacgcg ccacgagtcg atcgggttgg cgcccgccgc
     1501 gtcgtactcg gccgcccgcg gagcgatgtg ctcgcgagtc agccggtcga ccaactccag
     1561 caaggctttt tgggactggg ggggagcgac cgccgctcct cccccagacc cccccaccgg
     1621 tttcgagtca accgccatcg gccgctagct catgcccttg accggttggc ggttgccggt
     1681 gatgcagcgg caggtgccgg cctgatgcag ctcgatcatg ttgccgaacg ggtcgtacat
     1741 gaagatctgc tgcgactccg ggcccaccac gccccgggtc acccagtgct ccacgcccat
     1801 gcggtccagc tcggccttgg cctcctggat gtcgggcacc gcgagggcca cgtgcggccg
     1861 ggacggatcc ttgtccggcc cctgcgcgaa cttggactgc ccgtccacgc ccatgaggtg
     1921 gatctgggca gtgccgccca cgtccatcca gaagccgtcg atggttggaa taaccggacg
     1981 gccctggtcg gcgtcgaggc cgagcacgtc gtggtagaag ccgtaggcct tcttcacctc
     2041 ggcgggcgag ggaccgacgc ggataccgtg gtggtggagc tcaagcactt tgacggccat
     2101 gtatgcctcc tgtgagcgtg ccccatatcc ccttggggag gaacaggacg aacgcggaga
     2161 agatcgtgcc gatcaccagg ggccaggact cgaggacgag gctcagtcgc tcctccagca
     2221 ccaggaacgt ggcggcgccc acgaacggcc cgaagaaggt tccggccccg cccaggagcg
     2281 tcatcatgac cacctggccc gaggtggtcc agtatagcga ctcgaccggc acgacggtga
     2341 gccgcagcgc gtcgagcgat ccggcgagcc cggcgaagca ggcggagaa
//
LOCUS       DATMCV010000847         2840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_450152, whole genome shotgun sequence.
ACCESSION   DATMCV010000847 DATMCV010000000
VERSION     DATMCV010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450152"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..823)
                     /locus_tag="VNU03_19525"
     CDS             complement(164..823)
                     /locus_tag="VNU03_19525"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWP76721.1"
                     /translation="MGGKSEKPLVEKIRSHLEEQIIAGRLKPRQRLVEEEIAREMSVS
                     RSPIREALRALERDGLVTLTPGRGACVADLTPEEVDDVYAVRGRLGGLLFSLAARHIT
                     EDALARLDQVVAEMERVVADADIQRYFLLDLDFEEQVIGACPNKKLVVVWRNLGRPIL
                     RFRYFSLFPPGRLPTSLQYHRDLVVAFRRRDGATADQLVQRTIEAAGRALRDHLRSGV
                     A"
     gene            1115..1531
                     /locus_tag="VNU03_19530"
     CDS             1115..1531
                     /locus_tag="VNU03_19530"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWP76722.1"
                     /translation="MPPTNENAKKLEQAFVTWHVTKGKSTDIWLDLMAEDVRFHSLAG
                     GAPGAEFTVECRSRADFVRYLEGLTKDWEMIHYETSVFAADTDRVVMLGSTAWRNRRT
                     QKIVDTPKADLVTFRDGKIVDFSEFYDTAKLFAATV"
     gene            complement(1574..2134)
                     /locus_tag="VNU03_19535"
     CDS             complement(1574..2134)
                     /locus_tag="VNU03_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76723.1"
                     /translation="MRQGLLRGALVGLLIAPVAFGCVLPPDGGVSTPPEGQVVVADAR
                     ALAYGRSAGEILANPELRDKIRALFGADWAPPTGAGVGKLSAAAPQYFELGGPVRMVR
                     LGDANYITVTGCAAQACTARRGLLMIREGGEQLMARLDEGGFSHHYAYGPGTVGGPAG
                     AGPVLGSALRALQGVSDGSPYPRPAP"
     gene            complement(2179..2451)
                     /locus_tag="VNU03_19540"
     CDS             complement(2179..2451)
                     /locus_tag="VNU03_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76724.1"
                     /translation="MTRTRQMRALWVAPAFLAVAVLGCGAASAADKTKVDQATKQVEQ
                     GAKQIGDGSVGPGVKQTAKGIGNTVVEGAKYSGENIKEFFKKAFGS"
BASE COUNT          438 a         1014 c          934 g          454 t
ORIGIN      
        1 atccgggccc ggatgatctg ccacgcctgt cggagcttgg tgaaatcgtc ctcggtgctg
       61 cgcaggatcg ttccccgcgc atacgggagt ccggcggcga tgacgtttcc gaatcggtcg
      121 gtggccatgg ggctcttctc ctctcgatca ggcgggccgc cgctcacgcg acgccgctcc
      181 tgaggtggtc acgcagggcc cggcccgcgg cctcgatcgt ccgctggacg agctggtcgg
      241 cggtcgcgcc atcccggcgg cgaaacgcga cgacgaggtc gcggtggtac tggagcgagg
      301 tcggcaaccg gcccggggga aacagcgaga agtagcggaa acgcaggatc ggacgaccca
      361 ggtttcgcca gaccaccacg agcttcttgt tcgggcacgc cccgatcacc tgctcctcga
      421 agtcgagatc gaggagaaag taccgctgaa tgtccgcgtc ggccacgact cgctccatct
      481 cggcgaccac ctggtcgagc cgggccagcg cgtcttcggt gatgtgccgg gcggccagcg
      541 agaacaggag ccccccgagc cgcccgcgca ccgcgtagac gtcgtcgacc tcttccggcg
      601 tcaggtccgc gacgcaggcg ccgcggcccg gagtcagggt gacgagcccg tcgcgctcga
      661 gggcgcgcag cgcttcccgg atcggggagc ggctcaccga catctcgcgc gcgatctcct
      721 cctcgaccag ccgttgccgc ggcttgagac gacccgcgat gatctgctcc tcgaggtggg
      781 agcggatctt ctcgaccagc ggcttctcgg acttgcctcc catggggatc agacctttcg
      841 ccgccggtcg accaagcgcc tgcgggagcc ggcccactcg cgctcggcgg cccgagtcag
      901 gaaataccct gcgtccattg attaatcccg agatacttga ttgtcgacaa tgtcgaagac
      961 agtatcgccg tcgattcagc cagtcaagcc ccgcgtcgcc gcgagctagc tccccgcacc
     1021 cagtaagcac atgattcgat tgcctccggt tgccactcgg cctactgtcc tcccgcgtta
     1081 ctggctgtgc ctttcaccac acgggaggac cgcaatgccg ccgaccaacg agaacgcgaa
     1141 gaagctcgag caggccttcg tgacctggca cgtcacgaag ggcaagagca cggacatctg
     1201 gctcgacctg atggccgagg acgtgcggtt ccactcgctg gccggcgggg cgccgggcgc
     1261 ggagttcacc gtcgagtgcc gctcgcgcgc cgacttcgtg cgctacctgg aggggctcac
     1321 gaaggactgg gagatgatcc actacgagac gagcgtcttc gcggccgaca ccgatcgcgt
     1381 ggtcatgctc ggctccaccg cgtggcggaa ccgccggacc cagaagatcg tggacacccc
     1441 gaaggccgac ctcgtcacgt tccgtgacgg gaagatcgtc gacttctcgg agttctacga
     1501 caccgcgaag ctgttcgccg cgaccgtctg agccgggcgc ccggctagct gcctgacgcc
     1561 cggccccgcc gcgtcagggc gcggggcgtg ggtacgggct cccgtcggac acgccctgca
     1621 gcgcgcgcag cgcgctcccc agcaccggcc ccgcgccggc gggcccgccg accgtgccgg
     1681 ggccgtaggc gtagtggtgc gagaagccgc cctcgtcgag tcgcgccatc agctgctcgc
     1741 caccctcgcg gatcatcagg agcccgcgcc gggcggtgca ggcctgggcg gcgcagcccg
     1801 tgaccgtgat gtagttggcg tcccccagcc gaaccatgcg cacggggccg cccagctcga
     1861 agtactgcgg cgccgccgcg ctcagcttgc cgaccccggc tcccgtgggc ggcgcccagt
     1921 ccgcgccgaa gagcgcgcgg atcttgtcac ggagctccgg gttggccagg atctcgccgg
     1981 ccgatcggcc gtaggcgagc gcgcgggcgt cggccaccac cacctgtccc tcgggtggag
     2041 tggacacgcc gccgtccggc ggcagcacac agccgaaggc caccggcgcg atcagcaggc
     2101 ccacgagcgc tccccgcagc agtccctgtc gcatagacgg cctccttggc gcttctcgct
     2161 atgccacagg atacctgcct agctgccgaa cgccttcttg aagaattcct tgatgttctc
     2221 gccgctgtac ttggcgccct cgaccaccgt gttaccgatg cccttggcgg tctgcttcac
     2281 gcccggcccg accgagccgt cgccgatctg cttggcgccc tgctccacct gcttggtcgc
     2341 ctggtccacc ttggtcttgt cggcggcgct ggccgccccg cagcccagga ccgcgacggc
     2401 caggaaggcc ggcgcgaccc acaacgcgcg catctgccgt gtccgtgtca tggcttcgcc
     2461 tccttcgatc ggtttttaac cgaccggttt tcaacggaac cccagatacc cgagaacgaa
     2521 caggacaacc accacgacac cgatgatgta aaagatgttg ttcatggctc gctcctttct
     2581 ggggccaccc gcggcccgcg gtgtatcgcg tcgtgttgct tgccgccaag ggagcatcgg
     2641 ccatgccagg cgagggccgc ctgtatatac tgggacttgt cgcgcgcggt ggcgccgtgc
     2701 ccgccgggcg cggcgcgtcc tctgaaggaa ttaccgtgtc ctcgtgactt gggaccgaaa
     2761 ggatcgcgaa tgcctgggtc gcggagaacg cgggacgaca tccggatctc gaaccgggca
     2821 acgtactggc cggtgtcgcc
//
LOCUS       DATMCV010000848         2826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_450184, whole genome shotgun sequence.
ACCESSION   DATMCV010000848 DATMCV010000000
VERSION     DATMCV010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450184"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2569
                     /locus_tag="VNU03_19545"
     CDS             <1..2569
                     /locus_tag="VNU03_19545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP76725.1"
                     /translation="AALEGDYRDFFDLVAAQIGTAVTQASAYEEERKRAEALAELDRA
                     KTTFFSNISHEFRTPLTLMLGPLEDLLQGTPPGGRVEDRETLAVIHRNGRRLLKLVNA
                     LLDFSRIQAGRHDARYEPSDLGAVTTELAGVFRSAIEQAGLGFEVRCPAGLPAVYVDR
                     SMWEKIVLNLLSNAFKFTFEGTIAIELTDHGSSVDLAVRDSGIGIAPTEIDRVFDRFH
                     RVENARARTHEGSGIGLALVQELVRLHGGTVRVESAPGRGSTFTVTLPRGRAHLPDDR
                     IAAEATGADRSSGGGFFVEEALGWLPDADAAVDEPTAGAAADGEAAPRRSRILVVDDN
                     GDMREYVTRLLRSRWDVEAVGDGHAALASLRARPPGLVLADVMMPGLDGFALLSAVRA
                     DPATRATPVILLSARAGEEARVEGLEAGADDYVTKPFSARELLARVEATLRLRELRDR
                     AESDNARLLEKEQRARAAAEAASRAKDEFLAVLSHELRTPLNAVYGWARMLRAGEVEG
                     EAAKRALDVIMRNANAQVQLIDDMLDVSRIVSGKMRLDVRPVDLRMVVDAALDAVRPA
                     AEAKGLRLQAVLDPGALGISGDPDRLQQVVWNLLINAVKFTPREGRVQVQLHRVSSHV
                     EIIVSDTGQGIAPDVLPHVFERFQQADSGSARRHTGLGLGLALVRHLVELHGGAVEAS
                     SAGEGQGSVFTVKLPVALARVDAVGETPPPARVHTPAPAGASARGPSLSGLRVLVVDD
                     DRDGLDLVAMILLTSGAEVRACSSAADGFEALLAWRPDVLVSDIEMPGEDGYALIRRV
                     RGLESGAVARTPAVALSAYGRVEDRLRTLAAGYSMHVPKPVDPAELATIVASLAGRP"
     gene            complement(2578..>2826)
                     /locus_tag="VNU03_19550"
     CDS             complement(2578..>2826)
                     /locus_tag="VNU03_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76726.1"
                     /translation="FRARAWLRERGVPIHFEGRRRAGCQIAIEFRDPDGNNLEIYWNI
                     DQVGTVGGARPADEWRPAKTLEDAVANPVKGQTLPRRS"
BASE COUNT          359 a          992 c         1099 g          376 t
ORIGIN      
        1 cgcggcgctc gagggcgact accgcgactt cttcgatctc gttgccgcgc agatcggcac
       61 cgcggtgacc caggcgtcgg cctacgagga ggagcgcaag cgggccgagg ccctggccga
      121 gctcgaccgg gccaagacga cgttcttcag caacatcagc cacgagttcc ggaccccgct
      181 cacgctcatg ctcggtccgc tcgaggacct gctgcagggg acgccgccgg gcggacgagt
      241 ggaggaccgg gagacgctgg cggtgattca ccgcaacggc cggcggctgc tcaagctggt
      301 caacgcgctg ctcgatttct cccggatcca ggccgggcgg cacgacgccc gctacgagcc
      361 gagcgacctg ggcgcggtca ccacggagct ggcgggcgtg ttccggtcgg cgatcgagca
      421 ggcggggctc gggttcgagg tacggtgccc cgccggcctc cccgcggtct acgtcgatcg
      481 cagcatgtgg gagaagatcg tcctcaacct cctctcgaac gcgttcaagt tcaccttcga
      541 ggggacgatc gcgatcgagc tgaccgacca cggctcgtcg gtcgacctgg ccgtccgcga
      601 cagcggcatc ggcatcgcgc cgaccgagat cgaccgggtc ttcgatcgct tccaccgggt
      661 cgagaacgcg cgggcccgga cgcacgaggg ctccggcatc gggctcgcgc tggtgcagga
      721 gctggtgcgg ctgcacgggg gcacggtgcg cgtcgagagc gcgcccgggc ggggcagcac
      781 gttcacggtg acgctgcccc gggggcgggc gcaccttccg gacgaccgga tcgccgcgga
      841 ggccaccggc gccgaccggt cgagcggcgg cgggttcttc gtggaggagg cgctcgggtg
      901 gctgcccgac gccgacgccg cggtcgacga accgacggcg ggggcggccg cagacggcga
      961 ggcggccccg cgtcggagcc ggatcctggt ggtcgacgac aacggggaca tgcgcgagta
     1021 cgtgacccgc ctgctccggt cgcggtggga cgtcgaggcg gtgggcgacg gtcacgcggc
     1081 gctcgcgtcg ctccgggccc gtccgcccgg gctcgtgctg gccgacgtca tgatgcccgg
     1141 cctcgacggg ttcgcgctgc tgagcgcggt gcgcgccgat cccgcgacgc gggccacccc
     1201 ggtcatcctg ctctccgccc gggcgggcga ggaggcgcgg gtcgaggggc tcgaggcggg
     1261 ggccgacgac tacgtgacca agccgttctc ggcgcgggag ctcctggcgc gggtggaggc
     1321 gacgctccgg ctccgggagc tgcgcgaccg ggcggagagc gacaacgcgc ggctgctcga
     1381 gaaggagcag cgggcgcggg cggccgccga ggcggccagc cgggcgaagg acgagttcct
     1441 cgcggtgctc tcgcacgagc tgcgcacgcc gctcaacgcg gtctacggct gggcgcgcat
     1501 gctccgggcg ggggaggtcg agggcgaggc cgcgaagcgc gcgctcgacg tcatcatgcg
     1561 caacgccaac gcccaggtcc agctgatcga cgacatgctg gacgtctcgc gcatcgtctc
     1621 cggcaagatg cgcctcgacg tgcgcccggt ggacctccgg atggtggtgg acgcggcgct
     1681 cgatgcggtg cgccccgccg cggaggccaa gggcctgcgg ctgcaggcgg tgctcgatcc
     1741 cggcgcgctc gggatctccg gcgatccgga ccggctccag caagtcgtct ggaacctgct
     1801 catcaacgcg gtgaagttca ccccgcggga aggccgggtc caggtccagc tgcaccgggt
     1861 gagctcgcac gtcgagatca tcgtcagcga taccgggcag ggcatcgctc ccgacgtgct
     1921 gccccacgtg ttcgagcggt tccagcaggc cgacagcggg tcggcgcggc gccacaccgg
     1981 gctcggcctc ggcctggcgc tcgttcgcca cctggtggag ctgcacgggg gcgcagtcga
     2041 ggcgtccagt gccggcgagg gccagggctc cgtcttcacc gtcaagcttc cggtcgcgct
     2101 cgcgcgcgtc gatgcggtgg gggagacacc gccgcccgcg cgggtccaca cgcccgcgcc
     2161 cgccggggcg tcggcgcgcg ggccctcgct gagcgggctg cgggtgctgg tggtggacga
     2221 cgaccgggac gggctcgacc tggtcgcgat gatcctgctc accagcgggg ccgaggtgcg
     2281 agcctgctcg tcggccgcgg acggcttcga ggcgctgctg gcctggcgcc cggacgtcct
     2341 cgtctccgac atcgagatgc cgggtgagga cggctatgcg ctcatccgtc gggtgcgcgg
     2401 gctcgagagc ggtgcggtcg cccggacgcc cgcggtggcc ctcagcgcct acgggcgcgt
     2461 cgaggatcgg ctgcggacgc tcgcggcggg ctacagcatg cacgtcccga agccggtgga
     2521 tcccgccgag ctggccacca tcgtggcgag cctcgccggc cggccctagg cccgcgctca
     2581 gctgcggcgg gggagggtct ggcccttgac cgggttggcg accgcgtcct cgagagtctt
     2641 cgccggccgc cactcgtcgg cggggcgagc gcctcccacc gtgccgacct ggtcgatgtt
     2701 ccagtagatc tcgaggttgt tgccgtccgg gtcccggaac tcgatggcga tctggcagcc
     2761 ggcgcggcgg cggccctcga agtggatcgg cacgccccgc tcgcgcagcc acgcgcgggc
     2821 ccggaa
//
LOCUS       DATMCV010000849         2896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_44263, whole genome shotgun sequence.
ACCESSION   DATMCV010000849 DATMCV010000000
VERSION     DATMCV010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44263"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VNU03_19555"
     CDS             <1..1334
                     /locus_tag="VNU03_19555"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HWP76727.1"
                     /translation="AYTEMGEWALPPAAAAELTAARHRLQDLPDGERLARLLRGGFFR
                     NFLLKYPEIGDAYWKMLRLSRRVHEGLAARPRDPRLLAARERLWQGQANDAYWHGVFG
                     GCYLPHLRRAVKSALIGGERSLEGAGKPIGIEWTRADVDGDGQAEVTVRTRSLGVLMR
                     PARGGSLTELAWMGGEVDVADVLTRRPEAYHRQVAERPRESAPDEVKTIHSAPEVKEA
                     GLPALLRYDRFRRAVLLDGLFPPAEGRAPLDPLDPWDAAWMAVGERSLDHEVKASPRE
                     VAVLCSLLRPDGRPMALQKSLIVAADDPAVTAGYRLRWEGEEPLDARWAVQCNLTLSA
                     GDAPGRYFRVAGRPSLGSRGRLEGAHGLAMVDEWLGGEVALSFTAPAEVSWAPVETVS
                     LSESGFERIYQGSALLVAWPVRLAPGETWEVSLRVRVGEMAAGAPETGGPS"
     gene            1407..2558
                     /gene="metK"
                     /locus_tag="VNU03_19560"
     CDS             1407..2558
                     /gene="metK"
                     /locus_tag="VNU03_19560"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWP76728.1"
                     /translation="MAREEYLFTSESVTEGHPDKIADQVSDAVLDAILAQDPTGRVAC
                     ETLLTTGLVVVAGEITTSCYVDIPRVARETIREVGYTRAKFGFDHETCGVITAIDEQS
                     GDIAMGVDKLGAGDQGLMFGYACTETEELMPLPIMLAHKLCLRLTQVRKSGQLPYLRP
                     DGKSQVSVRYVDGKPVSVETVVISTQHGPETGLSKIREDMIEQVVLPTIPKHLIDPNR
                     VTYHINPTGRFVTGGPMGDTGLTGRKIIVDTYGGSCPHGGGAFSGKDPTKVDRSACYM
                     ARHVAKNVVAAGLAERAQVQVAYAIGVADPVSIMVETFGTGKVPNSKLEQMVRRHFDF
                     TPIGIIKYLDLRRPFYRKTAAYGHFGRNEPEFTWERTNRVKDLRDDAGV"
     gene            2717..>2896
                     /locus_tag="VNU03_19565"
     CDS             2717..>2896
                     /locus_tag="VNU03_19565"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWP76729.1"
                     /translation="MRRSRAGLWVLLGLVVLTVAGQAVPLYTDWLWFQEVGFTTVFTT
                     RLTISGWLFLGLGAAV"
BASE COUNT          405 a         1019 c         1063 g          409 t
ORIGIN      
        1 cggcctacac cgagatgggg gagtgggcgc tgcccccggc cgccgcggcg gagctcaccg
       61 cggcccgtca ccggctccag gatctgccgg acggcgagcg tctcgcgcga ctcctgcgcg
      121 gtgggttctt ccggaacttc ctgctcaagt acccggagat cggcgacgcc tactggaaga
      181 tgctgcgcct ctcccggagg gtgcacgagg gcctcgccgc gcggccccgc gatccgcgac
      241 tgctggccgc ccgtgagcgc ctgtggcagg gccaggccaa cgacgcctac tggcacggcg
      301 tgttcggggg ctgctacctg ccgcacctgc gccgggcggt gaagtcggcc ctgatcggcg
      361 gcgagcggag cctcgagggc gcgggcaagc cgatcggcat cgagtggacg cgcgcggacg
      421 tcgacggcga cgggcaggcc gaggtcaccg tccgcacccg ctccctcggg gtcctgatgc
      481 gtccggcccg cggcggatcg ctgaccgagc tcgcgtggat gggcggcgag gtggacgtgg
      541 ccgacgtgct gacccggcgg cccgaggcgt atcaccgcca ggtggcggag cgtccgcggg
      601 agagcgctcc ggacgaggtg aagacgatcc actccgctcc cgaggtgaag gaggcgggcc
      661 tgcccgcgct gctgcgctac gaccgcttcc gccgggcggt gctcctcgac gggctgttcc
      721 cgcccgcgga ggggcgcgcg ccgctcgacc cactcgatcc gtgggacgcg gcctggatgg
      781 cggtgggcga gcggtcgctc gaccacgagg tgaaggcctc gccccgcgag gtggccgtgc
      841 tctgctcgct gctgcgcccg gacggccgcc cgatggccct gcagaagtcg ctgatcgtcg
      901 cggcggacga ccccgcggtc accgcgggct accgcctgcg ctgggagggc gaggagccgc
      961 tcgacgcccg ctgggcggtg cagtgcaacc tcaccctcag cgcgggcgac gccccgggcc
     1021 gctatttccg ggtggccggc cggccgtcgc tcggcagccg cggtcgcctc gagggggcgc
     1081 acggcctcgc gatggtggac gagtggctcg gcggcgaagt ggccttgagc ttcaccgcgc
     1141 ccgccgaggt ctcgtgggcg ccggtcgaga cggtctcgct ctccgagagc ggcttcgagc
     1201 gcatctacca gggctcggcc ctgctcgtgg cctggccggt gcggctcgct cccggcgaga
     1261 cctgggaggt ctcgctgcgc gtgcgcgtcg gggagatggc ggcgggcgcg ccggaaaccg
     1321 gggggccctc ttgagagcgt gggaggggcg tgctaatgtc acgtattcgc agtcgatccc
     1381 tgacgaagcg cggagggcac tgaggcatgg cgcgtgagga gtacctgttc acctcggagt
     1441 cggtgaccga aggacatccg gacaagatcg ccgaccaggt ctcggatgcg gtcctcgacg
     1501 cgatcctggc gcaggatccc accggccgcg tcgcctgcga gaccctcctc accaccgggc
     1561 tcgtggtggt ggcgggcgag atcacgacct cctgctacgt cgacatcccg cgcgtggcgc
     1621 gcgagaccat ccgggaagtc ggctatacgc gcgccaagtt cggcttcgac cacgagacgt
     1681 gcggcgtcat caccgcgatc gacgagcagt cgggcgacat cgcgatgggg gtggacaagc
     1741 tgggcgcggg cgaccagggg ctcatgttcg gctacgcctg caccgagacc gaggagctga
     1801 tgcccctgcc gatcatgctg gcccacaagc tctgcctgcg gctcacccag gtgcggaaga
     1861 gtgggcagct cccctacctg cggccggacg gcaagagcca ggtgagcgtc cgctacgtgg
     1921 acggcaagcc ggtgtcggtg gagacggtgg tcatctcgac gcagcacggg cccgagaccg
     1981 gcctctccaa gatccgcgag gacatgatcg agcaggtggt gctgccgacg atccccaagc
     2041 acctgatcga tcccaaccgg gtcacctacc acatcaaccc caccggccgc ttcgtgaccg
     2101 gcggccccat gggcgacacc gggctcaccg ggcgcaagat catcgtcgac acctacggcg
     2161 gctcgtgccc ccacggcggc ggcgcgttct ccggcaagga cccgacgaag gtcgatcgct
     2221 ccgcgtgcta catggcccgc cacgtggcca agaacgtggt ggccgcgggc ctggccgagc
     2281 gggcgcaggt gcaggtggcc tacgcgatcg gggtggccga tccggtctcg atcatggtcg
     2341 agaccttcgg gaccgggaag gtgccgaaca gcaagctcga gcagatggtc cgccggcact
     2401 tcgacttcac ccccatcggc atcatcaagt acctggacct gcggcggccg ttctaccgca
     2461 agacggcggc ctacggacac ttcgggcgca acgagcccga gttcacgtgg gagcgcacga
     2521 atcgcgtcaa ggatcttcgg gacgacgccg gcgtctgacg ccggcctccg ctcacgcccg
     2581 catcccgcgg cccacggccc gggcccatga ctgaccacga cgtcaccgac cgctcgcggg
     2641 ccgtggccgg cgagatgcgc cggacgtaag gacggagcgc ttggcgcaga tgccccccgg
     2701 cggtcgagtc atacccttgc ggcgctcgcg ggccggtctc tgggtcctcc tgggcctggt
     2761 cgtcctcacc gtggcgggcc aggcggttcc gctctacacg gactggctgt ggttccagga
     2821 ggtcggcttc accaccgtct tcaccacgcg gctgacgatc agcggctggc tcttcctcgg
     2881 gctgggcgcc gcggtc
//
LOCUS       DATMCV010000850         3648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_980935, whole genome shotgun sequence.
ACCESSION   DATMCV010000850 DATMCV010000000
VERSION     DATMCV010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980935"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2586)
                     /locus_tag="VNU03_19570"
     CDS             complement(<1..2586)
                     /locus_tag="VNU03_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP76730.1"
                     /translation="MSLSEPFVRRPIATTLLTVGVALAGTLAFFRLPVSPLPQVDYPT
                     ISVQASQPGASPAVMATSVATPLERHLGTIANVTEMTSSSSLGSTRITLQFGLDRDIN
                     GAARDVQAAINAAHADLPASLRSNPTYRKVNPADAPIMILALTSTTLSQGQLYDAAAT
                     ILQQKLSQVSGIGQVDLGGSSLPAVRVELEPRALFRYGIGLEDVRAAIAAANANSPKG
                     AIEVGGRHYQLYTNDQARRADQYKGLVIAYRNGAAVRLADLAEVVDSVEDLRNQGLAN
                     GRPSVLVILSREPGANIIDTVDRVKDVLPQLRASIPAGAELAVASDRTTTIRASLRDV
                     EYTLVIAIALVILVVFVFLRSVRATIIPGVAVPISLIGTLGVMYLLGYSINNLSLMAL
                     TIATGFVVDDAIVVMENVTRYVEAGVPRLEAALRGAAEVGFTVLSMSLSLIAVFIPIL
                     LMSGILGRLFREFAITLSVAILVSLAVSLTTTPMMCAYFIPDRPERRGRLYRWSERGF
                     DWLVAAYGQTLDWALRHSLAVVLILAGVLALNAYLYLVIPKGFFPQQDTGRLTGRIQA
                     DQSTSFQLMRGKLRQFVDVVGEDPAVDAVVGFTGGGQTNGGSVFVSLKPRAERGVSVD
                     QVMARLRGKLARVPGATLFLQAVQDIRVGGRQSNALYQYTLQGDSLDDLRSWTPRLAD
                     ALGRVPEIVDVNSDQQDRGLQVTVQIDRPTASRLGISASQIDNTLYDAFGQRQVSTIY
                     NPLNQYHVVMEVAPRYWQSPEILRELYVSTAAGGVRGAQSTNAVAGTVSSASVTASAV
                     AADTARNQAANQLANTGRGNTSTGAAVATVAETMVPLVAFSSFGPDTTPLAVNHQGHF
                     VASTIS"
     gene            complement(2583..>3648)
                     /locus_tag="VNU03_19575"
     CDS             complement(2583..>3648)
                     /locus_tag="VNU03_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP76731.1"
                     /translation="EFTVWIPRLIDRMNALPHVEDVASDLQNQGLSVYVEVDRATAGR
                     FGITPATVDNALYDSFGQRIVSTVFTQSNQYRVILEADPALQESLQSLGSIYLPSSVA
                     AGGQVPLSAIASVREESAPLAVNHLGQFPAATVSFNLAPGASLGAAVDAITAAGREIG
                     LPASMASSFQGAALAFQASLANELFLILAALAAVYIILGVLYESYVHPVTILSTLPSA
                     GVGALLALMICHEDLGIIGIIGIILLIGIVKKNAIMMIDFALDAERSEGKSPREAIHQ
                     ACLLRLRPILMTTMAALLSALPLMLGTGTGAELRRPLGITIVGGLIVSQVLTLFTTPV
                     IYLAFDRLGRWARGRGSAAA"
BASE COUNT          547 a         1187 c         1411 g          503 t
ORIGIN      
        1 cgagatcgtc gaggccacga agtgcccctg gtggttgacc gcgagcgggg tggtgtcggg
       61 gccgaacgag ctgaacgcga ccagcggcac catggtctcc gccacggtcg ccaccgcggc
      121 gccggtcgag gtgttgccgc gcccggtgtt ggcgagctgg ttggccgcct ggttgcgcgc
      181 ggtgtccgcg gccaccgccg acgcggtgac gctcgcgctc gacacggtgc cggccaccgc
      241 gttggtggac tgggcgccgc gcaccccgcc cgccgcggtg ctgacgtaga gctcgcgcag
      301 gatctccggg ctctgccagt agcgcggcgc cacctccatc accacgtggt actggttgag
      361 cgggttgtag atggtggaga cctggcgctg gccgaaggcg tcgtagagcg tgttgtcgat
      421 ctggctcgcg ctgatgccga ggcgcgaggc ggtgggccgg tcgatctgca ccgtgacctg
      481 gagcccgcgg tcctgctggt cggagttgac gtcgacgatc tcgggcacgc ggccgagcgc
      541 gtcggccagc cggggcgtcc aggagcgcag gtcgtcgagg gagtcgccct gcagggtgta
      601 ctggtagagc gcgttgctct gccggccgcc cacccggatg tcctgcaccg cctgcaggaa
      661 cagggtggcg ccgggcaccc gcgcgagctt gccgcgcagg cgcgccatca cctggtcgac
      721 cgacacgccc cgctccgcgc ggggcttgag cgagacgaag accgacccgc cgttggtctg
      781 gccgccgccg gtgaagccga ccaccgcgtc gaccgcggga tcctccccca ccacgtcgac
      841 gaactgccgc agcttgccgc gcatgagctg gaaggatgtg ctctggtcgg cctggatgcg
      901 gccggtgagc cggccggtgt cctgctgcgg gaagaagccc ttggggatga cgaggtagag
      961 gtaggcgttg agggccagca ccccggccag gatgagcacc accgcgaggg agtgccgcag
     1021 ggcccagtcg agggtttggc cgtacgcggc caccagccag tcgaagccgc gctcgctcca
     1081 gcggtagagg cgaccgcggc gctcgggccg gtcggggatg aagtaggcgc acatcatcgg
     1141 ggtggtggtg agcgagaccg cgagcgacac caggatcgcg accgacaggg tgatcgcgaa
     1201 ctcgcggaag aggcggccca ggatgccgct catcaggagg atcgggatga agaccgcgat
     1261 gagggagagg ctcatcgaga gcaccgtgaa gcccacctcg gccgcgcccc gcagcgcggc
     1321 ctcgaggcgc ggcacgcccg cctcgacgta gcgggtcacg ttctccatca ccacgatcgc
     1381 gtcgtccacc acgaagccgg tggcgatggt cagcgccatc agcgagaggt tgttgatgct
     1441 gtagccgagc aggtacatga ccccgagcgt cccgatcagg gagatcggca ccgcgacgcc
     1501 gggaatgatg gtggcccgca cgctgcgcag gaacacgaac accaccagga tcaccagcgc
     1561 gatcgcgatg accagcgtgt actccacgtc gcgcagcgag gcccggatgg tggtggtgcg
     1621 gtcggaggcc accgcgagct cggccccggc gggaatggac gcgcgcagct ggggcagcac
     1681 gtccttcacc cggtcgacgg tgtcgatgat gttggcgccc ggctcgcgcg agaggatgac
     1741 cagcaccgac ggccgcccgt tggcgagacc ctggttgcgc aggtcctcca ccgagtccac
     1801 cacctcggcg aggtcggcga ggcgcaccgc cgcgccgttg cggtaggcga tcacgagccc
     1861 cttgtactgg tcggcccgcc gcgcctggtc gttggtgtag agctggtagt ggcggccgcc
     1921 cacctcgatg gcgcccttgg ggctgttggc gttggctgcc gcgatggccg cgcgcacgtc
     1981 ctcgaggccg atcccgtagc ggaagagcgc gcgcggctcg agctccaccc gcaccgcggg
     2041 cagcgagctg ccgcccaggt ccacctggcc gatgccggag acctgcgaga gcttctgctg
     2101 caggatcgtc gcagccgcgt cgtagagctg cccctggctg agggtggtgg aggtcagcgc
     2161 caggatcatg atgggggcgt cggccgggtt gaccttccgg taggtggggt tggaccgcag
     2221 gctcgcgggc aggtcggcgt gggccgcgtt gatcgcggcc tgcacgtcgc gggccgcccc
     2281 gttgatgtcg cggtcgaggc cgaactggag cgtgatgcgc gtcgagccga gcgagctgct
     2341 cgacgtcatc tcggtcacgt tggcgatggt gccgaggtgg cgctcgagcg gggtggccac
     2401 gctggtcgcc atcaccgcgg ggctcgcgcc gggctgggag gcctgcaccg agatggtcgg
     2461 gtagtccacc tgggggagcg gcgagacggg caggcggaag aaggcgaggg tgcccgcgag
     2521 ggccaccccg acggtcagca gggtggtggc gatgggccgc cggacgaagg gctcggagag
     2581 gctcacgcgg ccgcgctccc gcgtccgcgg gcccagcgcc cgagccggtc gaaggccagg
     2641 tagatgaccg gggtggtgaa gagggtcagg acctggctca cgatgagccc gcccacgatc
     2701 gtgatgccga gcggccgccg cagctccgcc ccggtgccgg tgccgagcat gaggggcagc
     2761 gcgctcagca gggccgccat ggtggtcatg agaatggggc gcaggcgcag caggcacgcc
     2821 tggtggatcg cctcccgggg cgacttgccc tcgctgcgct cggcgtccag cgcgaagtcg
     2881 atcatcatga tcgcgttctt cttcacgatg ccgatcagca gaatgatccc gatgatcccg
     2941 atgatgccga gatcctcgtg gcagatcatg agggccagca gcgcgccgac ccccgcggag
     3001 ggcagggtgg agaggatcgt gaccgggtgg acgtagctct cgtagagcac gcccaggatg
     3061 atgtagaccg cggcgagggc ggccaggatc aggaacagct cgttggcgag cgaggcctgg
     3121 aaggcgagcg cggcgccctg gaagctgctc gccatggacg cggggagccc gatctcgcgg
     3181 ccggccgcgg tgatcgcgtc gaccgccgcg ccgagcgagg cgcccggcgc caggttgaag
     3241 gagacggtgg cggccgggaa ctgccccagg tggttgaccg cgagcggcgc cgactcctcg
     3301 cgcaccgacg cgatggccga gagcggcacc tgcccgccgg ccgccaccga ggagggcagg
     3361 tagatggagc cgagcgactg cagcgactcc tggagcgcgg ggtccgcctc caggatgacg
     3421 cggtactggt tcgactgggt gaacacggtg gagacgatgc gctggccgaa ggagtcgtag
     3481 agcgcgttgt ccaccgtggc gggcgtgatg ccgaagcggc ccgcggtggc ccggtcgacc
     3541 tccacgtaga ccgagagccc ctggttctgg aggtcgctcg ccacgtcctc cacgtgcggc
     3601 agcgcgttca tgcggtcgat gaggcggggg atccacaccg tgaactcg
//
LOCUS       DATMCV010000851         5369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_580671, whole genome shotgun sequence.
ACCESSION   DATMCV010000851 DATMCV010000000
VERSION     DATMCV010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580671"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VNU03_19580"
     CDS             complement(<1..637)
                     /locus_tag="VNU03_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76732.1"
                     /translation="MTNAVAGLGLAPDAAMVTFPIEMFLPGSDIGPVEARKQEFYDGL
                     TRWRSAFAGDGAGEPPMLQVEGASVEDAFARANHLMLANRWGDGLPLWPPTRERVGWI
                     LRGTAEARGRKLGTFPPRGGVTTVESCAIALAMAGGRPEYLPVLVAAVEAFLDPESGS
                     ETLQAASGSAFPVVIVNGPIGARIRLNAGFGCLGPDPQRPAGASIGRALRLL"
     gene            complement(734..1027)
                     /locus_tag="VNU03_19585"
     CDS             complement(734..1027)
                     /locus_tag="VNU03_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76733.1"
                     /translation="MVRLEFHDPSGIIEIKRPHAARVPALAGKRIGFVSNEQWQAYRM
                     LPRLKALLETDFPGIEVLPIDAFPQGNAVIGSEETAALVKASGVDAVVIGNAA"
     gene            complement(1027..1347)
                     /locus_tag="VNU03_19590"
     CDS             complement(1027..1347)
                     /locus_tag="VNU03_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76734.1"
                     /translation="MAWAVIACVLAPAVALAQLPSIGSMLPDKAQLLEQAQKLVTELT
                     AMKSSGKLGAADASKVDSLLPKATAVNTELAKPQVPPSRLAQLAGQLGDLQKQAGALQ
                     GLMK"
     gene            1870..3123
                     /locus_tag="VNU03_19595"
     CDS             1870..3123
                     /locus_tag="VNU03_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76735.1"
                     /translation="MTGTLIANTMILDGSGAAPFPGDVLVEGQRIAAVQAGGGLPRGS
                     ARVIDGAGATLMPGLIEPHGHVSYPDAARNSDFTRLPPEEHVLVTMRNARTLLDCGYT
                     GVLSAAAAKPRLDIVIRNEIEAGRIPGPRYLANGPEITVTGGLGDENALHLPHLETPT
                     FAWVADGPDQIQRVCRILVREGADLLKLNISGDTGPRNSRSQRAVMTDAEVAAAMEIA
                     RAGAVRVCAHARSAESVKMCVRHGVGIIYHANFADEEALDLLEANRDWCFVAPAVGLT
                     YQACYAASEWGFTPQMARDHGLLDELEVSVDTMGRMRKRGIRVLPGGDYGFAWNPHGT
                     YARDLLLFVDLLGFSPMETLVATTRMGGEIMGRPQELGQVKPGYLADLLLVDGDPLTD
                     IKRLQRQDALLMIMKDGQLHKAPAA"
     gene            complement(3127..4371)
                     /locus_tag="VNU03_19600"
     CDS             complement(3127..4371)
                     /locus_tag="VNU03_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP76736.1"
                     /translation="MPTPATDAGSSEESAYAWTRLFAALLLSAIGGIGMWSVIVVLPA
                     VQVEFGVARSAASLPYTVTMISFGFGGILMGRLSDRYGICAPVVGGAIALSVGYLLAS
                     HTTSLWQFVLAQGLLVGVAGSASFAPLIADTSLWFTRHRGLAVSIISSGSYLAGTVWP
                     PVVQHFIQSIGWRRTYLGIAIFCVATMLPLALALRRRSPQLAPVASEAAPRATSQPSR
                     PLGMSPAALQTVLVIAGLSCCVAMSMPQVHMVAYAGDLGHGAARGAQMLSLMLATGIV
                     SRLASGWICDRIGGRRTLLLGSSLQALALVLFLPFDGLASLYILSALFGLSQGGITPA
                     YAVIIREFYPAEEAGVRVGTVLMATLFGMALGGWMSGVIYDLTGSYQAAFVNGILWNL
                     LNIGIAVGLLRQPGRPVQPAWR"
     gene            complement(4927..>5369)
                     /locus_tag="VNU03_19605"
     CDS             complement(4927..>5369)
                     /locus_tag="VNU03_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76737.1"
                     /translation="RFRILGCPARDEADALALEMVGHLLDPALYRLEVSGSGMLTAEL
                     VAWVDMHRPALVCIGAVAPGGLSQARHLCKRLRAQCPELKIVVGRWGLHDGKDTERQH
                     LLAAGADHVETRVLDTQRALAQVRLTVGRLAPESLPALVPAVVR"
BASE COUNT          861 a         1936 c         1858 g          714 t
ORIGIN      
        1 gcagcaggcg gagcgcgcgg ccgatgctgg cccccgccgg tcgctgcgga tcgggaccca
       61 ggcacccgaa gccggcattg agccggatcc gggcgccgat gggcccgttg acgatcacca
      121 ccggaaacgc gctgcccgac gcggcctgca atgtctcgct ccccgactcg ggatcgagga
      181 aggcctccac cgccgccacc agcacgggca ggtactcggg ccgcccgccc gccatcgcca
      241 gcgcgatggc gcacgactcg acggtggtga ccccgccacg cggtggaaac gtgccgagct
      301 ttcgcccgcg cgcttcggcg gtgccccgca ggatccagcc gacccgctcc cgcgtggggg
      361 gccagagggg caggccgtcg ccccagcggt tggccagcat caggtgattg gcccgcgcga
      421 acgcgtcctc gacgctcgcg ccctccacct gcagcatcgg cggctcgccc gcgccgtcgc
      481 cggcgaaggc cgagcgccag cgcgtcaggc cgtcgtagaa ctcctgcttg cgcgcctcca
      541 ccggcccgat gtcgctgccg ggcaagaaca tctcgatggg gaaggtgacc atcgccgcgt
      601 cgggcgccag cccgaggccg gcgaccgcgt tcgtcatgac cccgacgaaa tcagcccggg
      661 tcagggtgac ggtcggaatc ccctgtgcct cgagcctggc ggccgcccgg ccgcacgccg
      721 tcgcgcaggt ccctcaggcc gcgttgccga tgacgaccgc gtcgactccg ctcgccttga
      781 cgagggccgc cgtctcctcc gagccgatca ccgcgttgcc ctgggggaag gcgtcgatcg
      841 gcagcacctc gatgcccggg aagtccgtct cgagcagcgc cttgagacgc ggcaacatgc
      901 gataggcctg ccactgctcg ttgctgacga agccgatccg cttgcccgcg agcgcgggga
      961 cgcgcgcggc gtggggccgc ttgatctcga tgatgccgga cgggtcgtgg aactcgaggc
     1021 ggaccatcac ttcatcaagc cttggagcgc gcccgcctgc ttctggagat ccccgagctg
     1081 acccgcgagc tgggcgaggc ggctcggtgg gacctggggc ttcgccagct cggtgttgac
     1141 cgcggtcgcc ttggggagca gggagtccac cttcgaggcg tcggccgcgc cgagcttgcc
     1201 gctgctcttc atcgcggtca gctcggtgac cagcttctgg gcctgctcca ggagctgggc
     1261 cttgtccggc agcatcgagc cgatgctggg gagctgcgca agggcgaccg cgggggccag
     1321 cacgcaggcg atcacggccc acgccatcaa cgtcatccga ccattcaatc tgttcatcgt
     1381 cagaatcctc ccattgcgga gtataccgcc gggcgtcgcg gcgtcgccat caagcgcagc
     1441 ggcgacaccc tcttcgggct cacgatcgat cccggcaccg gccgcacgct gtcggtccag
     1501 ctggaccggg tgccctagga cccattgctg ccaccgctcg ccgacgctac aagtagggca
     1561 tgcgggcgat cggctccggc gtggcctgcc tcctgctcct cgccgcgact gtggcgcatg
     1621 cggatgagcc gtgggatctg tgggaggagc ggctcgtagg gaaggacccg gtccgcatgg
     1681 tcctggtcgg acacttcccg tcttcgcagg cctgccaggc tagagccgcg gagctgtgga
     1741 actccccggc acccgccggg atcacccggc tgggctacat ctgtctcccc gccgagcctc
     1801 cgtcccgccc cgacccgacc cccagccctc gctagaccgc gtcgtgtgac gtttgctaga
     1861 ctccgcccca tgaccggcac gctgatcgcg aacacgatga tcctcgacgg gagcggggcg
     1921 gcgccgttcc ccggcgacgt gctggtggaa ggccagcgca tcgcggcggt gcaggccggc
     1981 ggcggcctgc cgcgcgggtc ggcccgggtg atcgatggcg cgggcgccac gctcatgccg
     2041 gggctcatcg agccgcacgg gcacgtgagc tacccggacg cggcccgcaa ctccgacttc
     2101 acgcggctgc ctcccgagga gcacgtgctg gtgacgatgc gcaacgccag gacgctgctc
     2161 gactgcggct acaccggagt gctctcggcg gccgcggcca aaccacgcct ggacatcgtg
     2221 atccgcaacg agatcgaggc cggccgcatc ccgggaccgc gctacctggc caacggcccg
     2281 gagatcacgg tcacaggcgg gctcggcgac gagaacgcgc tccatctgcc gcacctggag
     2341 acgccgacct tcgcctgggt ggccgacggc ccggaccaga tccagcgggt ctgccgcatc
     2401 ctggtccgcg aaggcgccga cctcctgaag ctcaacatct cgggcgacac cgggccgcgc
     2461 aactcgcgct cccagcgcgc ggtgatgacc gacgccgagg tcgcggccgc gatggagatc
     2521 gcccgggccg gcgcggtgcg cgtctgcgcc cacgcgcgca gcgcggagtc cgtgaagatg
     2581 tgcgtgcggc acggggtggg catcatctac cacgccaact tcgcggacga ggaagcgctc
     2641 gacctgctcg aggccaaccg cgactggtgc ttcgtcgccc ccgcggtggg cctcacctac
     2701 caggcgtgct acgcggcgag cgagtggggg ttcacgcccc agatggcgcg cgaccacggg
     2761 ctgctggacg agctcgaggt gtcggtcgac accatgggcc ggatgcgcaa gcgcggcatc
     2821 cgcgtgctgc ccggcggcga ctacggcttc gcctggaacc cgcacgggac gtacgcgcgg
     2881 gacctgctgc tcttcgtcga cctgctcggc ttctctccga tggagaccct ggtcgcgacg
     2941 acgcgcatgg gcggcgagat catggggcgg ccccaggagc tggggcaggt gaagcccggc
     3001 tacctcgcag atctgctatt ggtcgacggc gatccgctca ccgacatcaa gcggctccag
     3061 cgacaggacg cgctgctgat gatcatgaag gacggccagc tgcacaaggc gccggccgcc
     3121 tgagggctac cgccaggccg gctggaccgg ccgtccgggc tgccgcagca gccccaccgc
     3181 gatgccgatg ttcagcaggt tccacaggat gccgttgacg aaggcggcct ggtaggagcc
     3241 ggtgaggtcg tagattacgc ccgacatcca cccgcccagg gccatgccga agagggtggc
     3301 catcagcacg gtcccgaccc gcaccccggc ctcctcggcc ggatagaact cccggatgat
     3361 gaccgcgtag gcgggcgtga tcccgccctg cgacagcccg aacagcgcgg acaggatgta
     3421 gagggacgcg agcccgtcga agggcaggaa gaggacgagg gccagcgcct gcaggctcga
     3481 gcccagcagc agcgtgcgcc ggcccccgat gcggtcgcag atccagcccg acgccagacg
     3541 gctcacgatg ccggtggcca gcatcagcga cagcatctgc gcgccccgcg ccgcgccgtg
     3601 gcccaggtcg cccgcgtacg cgaccatgtg gacctgcggc atcgacatcg cgacacagca
     3661 gctgaggccc gcgatgacca gcacggtctg cagagcggcc ggcgacatgc cgagcggccg
     3721 ggacggctgc gacgtggccc gcggggccgc ctcggaggcg acgggcgcga gctggggcga
     3781 ccggcgccgc agggccagcg cgagcggcag catcgtcgcc acgcagaaga tggcgatgcc
     3841 cagataggtc cgccgccagc cgatgctctg gatgaagtgc tgcaccaccg gcggccagac
     3901 cgtccccgcc aggtagctgc cgctcgagat gatcgacacc gccagcccgc gatggcgcgt
     3961 gaaccagagc gaagtatccg cgatgagcgg cgcgaacgag gccgagccgg cgacgccgac
     4021 gagcagcccc tgggccagaa cgaactgcca gaggctcgtc gtgtgactgg cgagcagata
     4081 gccgacgctc agggcgatcg ccccacccac gacgggcgcg cagatcccgt accgatcgga
     4141 gagccgcccc atcaggatgc cgccgaagcc gaaggagatc atcgtcacgg tgtagggcag
     4201 ggacgcggcg gagcgggcga cgccgaactc cacctggacc gccggcagca ccacgatcac
     4261 cgaccacatg ccgatgccgc cgatggccga cagcagcagc gcggcgaaga gccgggtcca
     4321 ggcgtaggcg gactcttcgg agctcccggc gtcggtggcg ggtgtgggca tggcgggcga
     4381 catccagtat ccgggttctc gcgcgatttg tactcagcca attcggcgtc gcgcggcgca
     4441 tgaaacggac gggcgcggag gcgcccggag cgcgggactc aggcccgaag tagcgtcgga
     4501 cgtcccagga cttgccacac tcgcgcacaa cgaggatgca tgtgcaggag ctgcttcggc
     4561 gaagcctcga cgccgaggta gttccgctgg ggaccaacga tgacctcgtc gcatgcggaa
     4621 cagacgaggc ccattcctgc ggacaccacc gccgcgcaga attgaaccgg agagccgagc
     4681 gcgcgggccc cgatcatacg cgcgatccgc ggctcgattc gcgcgcacgc ctcgcacgcg
     4741 ggcacgtcag ggatcaggac gtccgagcaa tacaggcagg atgtcgcggc gccttgggtc
     4801 gctctcatga ccgtcacctc tctgcgcaca tctaggccca taggttcgcc cctgagcatt
     4861 tcattaccta gcttttcctc agcaccccga agcattggac caaagtccta caccggtcag
     4921 aatggctcac cgcacgaccg ccggaaccag cgccggtagc gactcgggag ccaggcgccc
     4981 gaccgtaagt ctcacctggg cgagcgcccg ttgggtatcg agcaccctcg tctccacgtg
     5041 gtcggctccg gccgcgagga ggtgctggcg ctcggtatcc tttccgtcgt ggagacccca
     5101 acgaccgacc acgatcttga gctccggaca ctgcgcccgc aggcgcttgc agagatgccg
     5161 cgcctggctc aggcccccgg gggccaccgc cccgatgcag acgagtgcgg ggcgatgcat
     5221 gtcgacccag gcgaccaatt cggccgtgag catcccgctc ccgctcacct cgagccggta
     5281 cagcgccggg tcgagcagat gtccgaccat ctcgagcgcc aaggcatccg cctcgtcccg
     5341 agccgggcat cccaggatcc ggaagcggg
//
LOCUS       DATMCV010000852         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_112719, whole genome shotgun sequence.
ACCESSION   DATMCV010000852 DATMCV010000000
VERSION     DATMCV010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112719"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VNU03_19610"
     CDS             complement(<1..356)
                     /locus_tag="VNU03_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP76738.1"
                     /translation="MPEPVVDLRGVFKEFSGRVGRLVALEGIDLAIAPEEFTAVLGPS
                     GCGKSTLLNMVAGFDAPTRGEVLFRGQRVSRPDPRRGVVFQEAALFPWYTVLDNITFG
                     PKTGGVPAADYGPRVDA"
     gene            complement(349..1143)
                     /locus_tag="VNU03_19615"
     CDS             complement(349..1143)
                     /locus_tag="VNU03_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76739.1"
                     /translation="MGGRRVPLFLILPLLLLGVWYLAVEQRWVAEGIIPAPAQVLESW
                     RRWIFGGPTRSLSPYSGTWVANVLYSGRRVLEGFLLAAVVAIPLGIFVGWNRLVARVV
                     DPSIQLFRPIPITAWLPFAIAVFGIYDASALFLIGLGAFYPIAVNTSHGVRDTNLLLL
                     RAARMLGAGERTVLLKVVLPSALPSIFTGLRLGIGVAWTAVIVAEMIAVKSGLGYVLW
                     DAYYVGRMDICVATMFSVGLLGFISDQVILRASQVVLRWRTLERHA"
     gene            complement(1160..2185)
                     /locus_tag="VNU03_19620"
     CDS             complement(1160..2185)
                     /locus_tag="VNU03_19620"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76740.1"
                     /translation="MNVRRLGPTLALLAVLSAAASPTPAAAQSDPSRTRLAAPVKVKQ
                     GMANVPALSPLWLLPEEAAKYNVQVEQVIFQRFADERTALASGDLDIAAFGPQDIILG
                     LGQGAKSMIGVAGVGSGNDCLLVRKGEDVKDWAGVAPKRIGIGAGSISWLKFAAAVQE
                     NGLDYGKLRIVNIMGGGGNYLKSLQGKEIDMAVVWHPVCAQGITEGWAQYPAITHNAT
                     KTVGGLIAVLALNRGFMDKQREAAQRLVSAYVDVVKIAQADPQRFAKIYAEKAGLPEA
                     VAAEAIRYTRFDVTLPLASIKGIAKFLFDNGVIARDVAGEVEQYHTYEFLARATGRSA
                     ADLGQSK"
BASE COUNT          384 a          805 c          822 g          307 t
ORIGIN      
        1 gcgtcgaccc gcgggccgta gtcggcggcg ggcaccccgc cggtcttggg gccgaaggtg
       61 atgttgtcga gcacggtgta ccaggggaag agcgccgcct cctggaacac cacgccacgc
      121 cggggatcgg gacggctcac ccgctggccg cgaaagagca cctcgccgcg ggtgggcgcg
      181 tcgaagcccg cgaccatgtt cagcagcgtg gacttgccgc agcccgacgg cccgagaacc
      241 gcggtgaact cctcgggcgc gatcgcgagg tcgatgccct cgagcgcgac cagccggccg
      301 acccgcccgc tgaactcctt gaagacgccc cgcaggtcga ccaccggctc aggcatgccg
      361 ctcgagcgtg cgccagcgca acaccacctg ggaggcgcgg aggatgacct gatcggagat
      421 gaagcccagg aggcccaccg agaacatggt ggccacgcag atgtccatcc ggccgacgta
      481 gtaggcgtcc cagagcacgt aaccgaggcc ggacttgacc gcgatcatct ccgccacgat
      541 gaccgcggtc cacgccaccc cgatgccgag ccggagcccg gtgaagatgg acgggagggc
      601 cgacggcagc acgaccttca ggaggacggt ccgctccccc gccccgagca tgcgcgcggc
      661 ccgcaggagc aggaggttcg tgtcgcgcac cccgtgggag gtattgaccg cgatggggta
      721 gaaggctccg aggccgatca ggaagagcgc gctcgcgtcg tagatgccga agaccgcgat
      781 ggcgaacggc agccaggcgg tgatggggat cggccggaag agctggatcg agggatcgac
      841 cacgcgggcc accaaccggt tccagcccac gaagatcccg agggggatgg ctaccaccgc
      901 ggcgagcagg aacccttcca ggacccggcg gcccgagtag agcacgttgg cgacccaggt
      961 gcccgagtag ggcgagagcg accgggtcgg cccgccgaag atccaccgcc gccacgactc
     1021 gagcacctgc gcgggcgcgg ggatgatccc ctcggcgacc cagcgctgct cgaccgcgag
     1081 gtaccagacc ccgaggagca ggagcggcag gatcaggaag agagggacgc gccggccgcc
     1141 cacgcgcgcc cgcgcctcgc tacttgctct gcccgaggtc cgccgcgctg cgcccggtgg
     1201 cccgcgcgag aaactcgtag gtgtggtact gctccacctc gcccgcgacg tcgcgggcga
     1261 tgacgccgtt gtcgaagagg aacttcgcga tgcccttgat cgacgcgagc ggcagcgtca
     1321 cgtcgaagcg ggtgtagcgg atggcctccg cggcgaccgc ctccggcagc cccgccttct
     1381 ccgcgtagat cttggcgaag cgctgcggat cggcctgggc gatcttcacc acgtccacgt
     1441 aggccgagac cagccgctgc gcggcctcgc gctgcttgtc catgaagccg cggttgagcg
     1501 ccaggaccgc gatgagcccg cccaccgtct tcgtggcgtt gtgggtgatg gccgggtact
     1561 gggcccagcc ctccgtgatg ccctgggcgc agaccgggtg ccacaccacc gccatgtcga
     1621 tctccttgcc ctgcagcgac ttcaggtagt tgccgccacc tcccatgatg ttcacgatgc
     1681 ggagcttgcc gtagtcgaga ccgttttcct ggaccgcggc cgcgaacttg agccacgaga
     1741 tcgagccggc cccgatcccg atccgcttcg gggcgacccc ggcccagtcc ttgacgtcct
     1801 cgcccttgcg aaccagcagg cagtcgttgc cggagccgac gccggccact ccgatcatcg
     1861 acttcgcgcc ctggcccagg cccaggatga tgtcctgggg accgaacgcg gcgatgtcga
     1921 gatcgcccga ggcgagcgcg gtgcgctcgt ccgcgaagcg ctggaagatg acctgctcga
     1981 cctggacgtt gtacttcgcg gcctcctcgg gcaggagcca gagcggcgac agggcgggca
     2041 cgttcgccat cccctgcttc accttgaccg gggcggccag ccgggtccgc gaggggtccg
     2101 actgggccgc ggcgggagtc ggcgacgcgg cggcggagag aacggcgagc agcgcgagcg
     2161 tcgggccgag gcggcggacg ttcattcgtg ggctcctttc gcgagcacgt tgcgtagaat
     2221 gcgcgatggg aggagcctta ggcaagcatg gaaatgagca ttctgaagcg cctgatcgtg
     2281 ggcgctccca tgcctctcgc ccaggcccgg cacgagcg
//
LOCUS       DATMCV010000853         4359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_777873, whole genome shotgun sequence.
ACCESSION   DATMCV010000853 DATMCV010000000
VERSION     DATMCV010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777873"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1462
                     /locus_tag="VNU03_19625"
     CDS             182..1462
                     /locus_tag="VNU03_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008388290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWP76741.1"
                     /translation="MKQPWRPRVLIGVVISFVIGAVVLASAGMFVARAADPIKIGFGM
                     ALTGGLSANGKPALLALQIWKDDINKKGGLLGRPVELVFYDDQTNPATVPGIYSKLLD
                     VDKVDIVTSGYGTNLIAPLMPIAMERKLMIMGIFGLANNEKYKYPNYFQISPNGPEPE
                     TSTALGFFELAARQNPKPQTVAIVGADAEYPQNALVGARELIKKFGFKTVYDKTYPPS
                     TTDFTPIVRAIKATNPDVVFVASYPPDSVGMLRAAHEVGLQPKLFGGGMVGLMFTSIM
                     TSMGPMLNGVVNYDFWAPEPTFLAMPGIKEFLKEYQARAEKAGVDPLGYYLPPYSYAT
                     GQVLAQAIEATKGLDQQKLADYIRATEFNTIVGKVKFGKNGEWAKGRTLMVQYQKIQG
                     NDIEQFRGPGKKVVLYPDEFKSGNIIYPYSAAKN"
     gene            1506..2348
                     /locus_tag="VNU03_19630"
     CDS             1506..2348
                     /locus_tag="VNU03_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76742.1"
                     /translation="MVAGLLLGGFYAAVSLGISLVFGLLDIANIAQPAFLILGAYSAY
                     VMNGSFGLDPILAGVLVTPLFYGLGVLVYRVYYESFERRGEEALRGLVFFFGLLFIIE
                     VSLSLKYGVDYRLVQADYIGRSIEFGGVGIAYRLLVPCLVGLAMTVALYFFLARTFYG
                     RAIMAVSQDAGALRLMGADPIRIKTIAFGIGIAAASLAGALAIMIAPVVPSSDRDYIG
                     RMFAITVLGGMGSIGGTLVAAIILGVVESLMGTFFGPSWSPAVSFGILLIALAVRPAG
                     LFGR"
     gene            2349..3284
                     /locus_tag="VNU03_19635"
     CDS             2349..3284
                     /locus_tag="VNU03_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76743.1"
                     /translation="MRRTGLILLVGAAVVAGGILLASLRVNPYFYFAGYVILQYIVIA
                     TAWNILGGYAGYVNFGTPAFFALGAYTAVFLIQSMRAPLPVLILAGGLVSALLGLGIG
                     YLTLRLRGVFFSIATLALSIVLQTMIINWEFVGGSRGLSVIRPSGPPFGNYVTFLFTV
                     MVLLAVVSVVTARFIERSWIGRGLAALRDNEEAAECMGVPTLRLKLFATTISGFLLGI
                     AGAPFPYYVTFVDPNSAFSLDYAVNALAMPMIGGTTSWVGPVIGAVLLGTAQQLATVT
                     ISSEMNLFIVGVVLVAFVVLAPEGILGLVRRLRRR"
     gene            3288..4007
                     /locus_tag="VNU03_19640"
     CDS             3288..4007
                     /locus_tag="VNU03_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76744.1"
                     /translation="MPPALLEVNRLTKRFGGFMALHEVTIHVKAGERFGLIGPNGSGK
                     TTLINCVSGSLPADGGRIVFDGRDITGLAAHRRTRLGLVRSFQIPKPFSSMTVLENLD
                     IPLEYAAHERSDAADADAMEILRAIGLESKAHLRPAGLTQIEMRKLELARAMAARPKL
                     LISDEAMAGLSHAEVDDILAILFGLSERGITIIMIEHIMRAVMRFSERIVVLDAGELI
                     AEGTPDEIVRNPAVEKAYLGE"
     gene            3997..>4359
                     /locus_tag="VNU03_19645"
     CDS             3997..>4359
                     /locus_tag="VNU03_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP76745.1"
                     /translation="MASRIVVRDVDAGYGAVRVLHGVSIEVREGETVALLGTNGNGKS
                     TLIKCLMGMVRPSSGEIFLETDAGRVDLAGKSTEEIVGLGIALVPEGRRLFPKLSVEE
                     NLLLGAYRREARADIAANL"
BASE COUNT          653 a         1493 c         1475 g          738 t
ORIGIN      
        1 gggcagcctg gccatggggg ctcctcctct cccgggacta tagacgatcg cctcccgagc
       61 cgcccactgc cttgacaagg gcgcggcccc gggcgcagtc ttggcccgcc caatgcccgg
      121 cgcgctccaa cgggggcgcg cgccaagaga ggccaagagg ggccaagaga ggaagaatgc
      181 gatgaagcag ccctggagac cgcgggtcct gatcggggtc gtcatctcgt tcgtcatcgg
      241 cgcggtcgtg ctggccagcg cggggatgtt cgtcgcgcgg gccgccgatc ccatcaagat
      301 cggcttcggc atggcgctga ccggcgggct ctccgccaac ggcaagccgg cgctgctggc
      361 gctgcagatc tggaaggacg acatcaacaa gaagggcggt ctcctcggcc ggccggtgga
      421 gctggtcttc tacgacgacc agaccaaccc ggccaccgtg cccggcatct actcgaagct
      481 gctcgacgtc gacaaggtgg acatcgtcac ctccggctac ggcaccaacc tcatcgcgcc
      541 gctcatgccc atcgccatgg agcgcaagct catgatcatg gggatcttcg ggctcgcgaa
      601 caacgagaag tacaagtacc cgaactactt ccagatctcg cccaacgggc cggagccgga
      661 gacgagcacc gcgctcggct tcttcgagct ggccgctcgc cagaacccca agccccagac
      721 ggtggccatc gtgggcgccg acgcggagta cccgcagaac gcgctggtgg gggcgcgcga
      781 gctgatcaag aagttcggct tcaagacggt ctacgacaag acctatccgc ccagcaccac
      841 cgacttcacc ccgatcgtgc gcgcgatcaa ggcgacgaat cccgacgtgg tgttcgtggc
      901 ctcgtacccg ccggactcgg tgggcatgct gcgcgcggcc cacgaggtgg ggctccagcc
      961 caagctcttc ggcggcggca tggtgggcct gatgttcacg agcatcatga ccagcatggg
     1021 gccgatgctc aacggcgtcg tgaactacga cttctgggct ccggagccga ccttcctcgc
     1081 gatgcccggg atcaaggagt tcttgaagga gtaccaggcc cgcgccgaga aggcgggggt
     1141 cgatccgctc ggctattacc tgccgcccta ctcgtacgcg accgggcagg tgctggccca
     1201 ggccatcgag gccaccaagg gcctcgacca gcagaagctg gccgactata tccgggccac
     1261 cgagttcaac accatcgtgg gcaaggtgaa gttcggcaag aacggggagt gggccaaggg
     1321 gcgcaccctg atggtccagt accagaagat ccagggcaac gacatcgagc agttccgcgg
     1381 gccgggcaag aaggtcgtgc tctatcccga cgagttcaag tccggcaaca tcatttatcc
     1441 gtactcggcg gcgaagaact agcgcgagtc gctccctgtt ctcctgggac ctgctggcga
     1501 acgcgctggt cgccggactt ctcctcgggg gcttctatgc cgcggtgagc ctcggcatct
     1561 cgctcgtctt cggcctgctc gacatcgcca acatcgcgca gcccgcgttc ctgatcctgg
     1621 gcgcgtactc ggcgtacgtg atgaacggct cgttcggcct cgaccccatc ctggccggcg
     1681 tgctcgtcac ccccctcttc tacgggctcg gggttctcgt ctatcgcgtc tactacgaga
     1741 gcttcgagcg gcggggggag gaggcgctcc ggggcctcgt cttcttcttc gggctcctgt
     1801 tcatcatcga ggtgagcctc agcctgaagt acggcgtcga ctaccggctg gttcaggccg
     1861 actacatcgg ccggtcgatc gagttcggcg gggtcgggat cgcgtaccgg ctgctggtgc
     1921 cgtgcctcgt cgggctcgcc atgacggtgg cgctctactt cttcctggcc cggacgttct
     1981 acgggcgggc gatcatggcg gtctcgcagg acgcgggcgc gctgcgcctg atgggggccg
     2041 acccgatccg gatcaagacc atcgccttcg ggatcgggat cgcggcggcg agcctggccg
     2101 gcgcgctcgc gatcatgatc gcgccggtgg tgccgtcgag cgaccgcgac tacatcggcc
     2161 ggatgttcgc gatcacggta ctgggcggca tgggcagcat cggcggcacc ctggtcgcgg
     2221 cgatcatcct gggggtggtc gagagcctga tgggcacatt cttcggcccg tcgtggtctc
     2281 ccgcggtgtc gttcggtatc ctactgatcg cgctcgcggt gcggccggcc gggctcttcg
     2341 gacgctagat gcggcgcacc ggcctgatcc tcctggtggg cgcggcggtg gtggcggggg
     2401 gaatcctcct cgcctccctg cgggtgaacc cgtacttcta cttcgccggc tacgtgatcc
     2461 tccagtacat cgtgatcgcc accgcgtgga acatcctggg cggctacgcg ggctacgtga
     2521 acttcggcac tcccgcgttc ttcgccctcg gggcctacac cgcggtcttc ctcatccagt
     2581 cgatgcgggc gccgctgccg gtgctgatcc tggccggcgg gctggtctcg gccctgctcg
     2641 gcctcggcat cggctatctc accctgcggc tccggggggt cttcttctcc atcgcgacgc
     2701 tggcgctctc catcgtgctc cagaccatga tcatcaactg ggagttcgtg ggcggctccc
     2761 ggggcctcag cgtcatccgc ccgagcgggc cgcccttcgg caactacgtg acgttcctct
     2821 tcacggtcat ggtcctgctc gcggtcgtct cggtggtcac cgcccggttc atcgagcgct
     2881 cctggatcgg ccgcggcctg gccgcgctgc gcgacaacga ggaggccgcc gagtgcatgg
     2941 gggtgccgac cctgcgcctc aagctcttcg ccaccacgat cagcggcttc ctcctcggga
     3001 tcgcgggggc cccgttcccg tactacgtga ccttcgtgga ccccaactcc gccttcagcc
     3061 tcgactacgc ggtgaacgcg ctcgcgatgc cgatgatcgg ggggaccacg agctgggtgg
     3121 gcccggtgat cggggcggtg ctgctcggga ccgcccagca gctcgccacc gtgaccatct
     3181 cctcggagat gaacctcttc atcgtgggcg tggtgctggt ggccttcgtg gtcctggccc
     3241 ccgaaggcat cctgggcctg gtgcggcgcc tgcggaggcg atgaccgatg ccccccgccc
     3301 tgctcgaggt caaccgtctc accaagcgct tcggcggctt catggcgctc cacgaggtca
     3361 ccatccacgt gaaggcgggc gagcggttcg ggctgatcgg gcccaacggc tcgggcaaga
     3421 cgaccctcat caactgcgtg tcggggtcgc tcccggcgga cggcggccgc atcgtgttcg
     3481 acggccggga catcaccggc ctggcggccc accggcgcac gcggctcggg ctcgtccgca
     3541 gcttccagat ccccaagccg ttctccagca tgacggtgct ggagaacctc gacatcccgc
     3601 tcgagtacgc ggcgcacgag cgctccgacg cggccgacgc ggacgcgatg gagatcctgc
     3661 gcgccatcgg gctcgagtcg aaggcccacc tgcggccggc cgggctcacc cagatcgaga
     3721 tgcgcaagct cgagctggcc cgggcgatgg cggcgcggcc gaagctgctc atctccgacg
     3781 aggcgatggc cgggctctcg cacgcggagg tggacgacat cctcgccatc ctgttcggcc
     3841 tgagcgagcg gggcatcacg atcatcatga tcgagcacat catgcgggcg gtgatgcgct
     3901 tctccgagcg catcgtggtg ctcgacgcgg gcgagctgat cgcggagggc accccggacg
     3961 agatcgtccg caaccccgcg gtggagaaag cctaccttgg cgagtaggat cgtggtccgc
     4021 gacgtggacg ccggctacgg cgcggtgcgg gtgctccacg gggtgtcgat cgaggtgcgg
     4081 gaaggggaga ccgtcgccct gctcggcacc aacggcaacg gcaagagcac gctcatcaag
     4141 tgcctgatgg gcatggtgcg accctcgtcg ggcgagatct tcctcgagac cgacgccggg
     4201 cgcgtggatc tcgccggcaa gtccaccgag gagatcgtgg gcctcggcat cgcgctggtg
     4261 ccggagggac gccggctctt cccgaagctc tcggtggagg agaacctgct cctgggcgcc
     4321 taccgtcgcg aggcccgcgc cgacatcgcg gccaacctc
//
LOCUS       DATMCV010000854         3997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_313242, whole genome shotgun sequence.
ACCESSION   DATMCV010000854 DATMCV010000000
VERSION     DATMCV010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313242"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="VNU03_19650"
     CDS             complement(<1..1599)
                     /locus_tag="VNU03_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009181043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP76746.1"
                     /translation="MNPSRPFILRPVATSLLTAAIVLAGLVAYRLLAVSALPQVDYPT
                     MQVQTFYPGASPDVMASAVTAPLERQFGQMPGLKQMTSTSSSTSSVITLQFDLNLALD
                     IAEQQVQAAINAATSFLPTDLPNPPIYSKVNPADAPILTLGLTSTALPLTSVQDLADT
                     RLVPKISQLPGVGLVTLSGGQKPAIRIQVNPARLAANALTLEDVRIAVANASVNQAKG
                     SLDGPRQAFTIGANDQLVSSEQYRNLVIAYRGGAPVHLSDVADTVDDAENLRQAAWMG
                     AEPAVIVNIQRQPGANLIQVVDRVKLLLPTLQATLPSSVKVAILSDRTTTIRASVHDV
                     QLELMLAVVLVVAVIFLFLGSPAATFIPSIAVPVSIIGTFAVMYALNFSLNNLSLMAL
                     TIATGFVVDDAVVMIENIARYIEAGDSPLEAALKGSEQIGFTILSLTVSLIAVLIPLL
                     FMGDIVGRLFREFAITLSAAILVSAAVSLTLTPMLCAKLLRHHEPHDTGRVARASQAV
                     FNRVLDLYARTLRVVLAHQGLTLVV"
     gene            complement(1596..2834)
                     /locus_tag="VNU03_19655"
     CDS             complement(1596..2834)
                     /locus_tag="VNU03_19655"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HWP76747.1"
                     /translation="MAGEAHESGEFRPTPSDAPRRSSGRLWVIGIVLAGLLAGGGYFA
                     LNRPGRAQPAASTGPRAVPVVTVPARTGDLPVYLTGLGSVTALNTVTVRSRVDGQLMR
                     TSFQEGQIVKAGDVLAEIDPRPFEAQLTQVEGQLAKDEAALSNARLDLERYKTLVKGG
                     FIPQQQLDTQASSVSQIAASIKADQGNIDAIRLQLTYCRILAPITGRTGLRLVDAGNM
                     VHANDAGGLVVLTQVEPIAVLFTIPQDNLQSVLARQRAAGAIAVEAFDREGRNRIGSG
                     TLVAVDNQIDQGTGTVKIKAAFENADGMLYPNQFVNARVLVDTLKGAVLVPNEAIQRG
                     AQGAFVYVVKPDKTVELRKVALGPAEGGVTAVREGVAAGDALIVDGADKVQERSRVEP
                     TTRGERPPARGERPPGRTGS"
     gene            complement(2966..3955)
                     /locus_tag="VNU03_19660"
     CDS             complement(2966..3955)
                     /locus_tag="VNU03_19660"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWP76748.1"
                     /translation="MLEQAFTCSEPLAAIRERDRDGDLWAVVLAGGEGSRLRALTRYL
                     YGDERPKQYAVLTGSKSLLRQTLERVARLVPPSRIVVVAQASHDRYLGAELAGFPQIH
                     VLAQPSDRGTAAGVLMPAHWIRARDPRAVVAVFPADHFILEEETFMRHVGEVAGYVRA
                     HPEGLVLLGVPPSDADPDYSWIEPGVRLGWAGPDPVHRIRGFREKPTEESARRLYALG
                     GLWNTFVFAASVTTLIEAGRECVPLLHDRLVRMGLFSGTQYEAWALRQAYLFAPAADF
                     SRTVLESPSLALAVAEVPTHTWCDLGTPERVAWTLRALGAPPPAWLGLLGSTS"
BASE COUNT          574 a         1317 c         1536 g          570 t
ORIGIN      
        1 caccaccagg gtcagcccct ggtgcgcgag gaccacccgc agggtgcgcg cgtagaggtc
       61 gagcacgcgg ttgaacaccg cctgggaggc gcgcgcgacc cggccggtat cgtgcggctc
      121 gtgatggcgc agcagcttgg cgcagagcat cggggtgagc gtgagggaca ccgcggccga
      181 gacgaggatc gcggcgctga gggtgatcgc gaactcccgg aagagccggc ccacgatgtc
      241 gcccatgaac aggagcggga tcagcaccgc gatgagggag acggtcagcg acaggatggt
      301 gaacccgatc tgctcggagc ccttgagggc ggcttcgagc ggggagtcgc ccgcctcgat
      361 gtagcgcgcg atgttctcga tcatcaccac cgcgtcgtcc accacgaagc cggtggcgat
      421 ggtgagcgcc atcagggaga ggttgttcag gctgaagttg agcgcgtaca tgaccgcgaa
      481 ggtgccgatg atcgagaccg gcaccgcgat cgacgggatg aaggtggcgg ccgggctgcc
      541 caggaacagg aagatcaccg ccaccaccag gaccaccgcg agcatcagct cgagctgcac
      601 gtcgtggacg gaggcgcgga tggtggtggt gcggtcgctc aggatcgcca ccttgaccga
      661 gctgggcagg gtggcctgga gcgtgggcag cagcagcttg acgcggtcga ccacctggat
      721 caggttggcg ccgggctgcc gctggatgtt gacgatcacc gcgggctccg cgcccatcca
      781 ggcggcctgg cgcaggttct ccgcgtcgtc cacggtgtcg gccacgtccg agaggtgcac
      841 cggcgcgccg ccgcggtacg cgatgacgag gttgcggtac tgctcgctgc tgacgagctg
      901 gtcgttggcc ccgatggtga aggcctggcg cgggccgtcg aggctgccct tggcctggtt
      961 cacgctcgcg ttggccaccg cgatccgcac gtcctcgagg gtgagcgcgt tggcggccag
     1021 ccgcgcgggg ttcacctgga tgcggatcgc cggcttctgg ccgccgctca gggtcaccag
     1081 gcccacgccc gggagctggg agatcttcgg caccaggcgc gtgtccgcga ggtcctggac
     1141 gctcgtcaag ggcagggcgg tcgaggtgag acccagggtc aggatcggcg cgtcggccgg
     1201 gttcaccttg ctgtagatcg gcgggttggg gagatcggtg ggcagaaagg aggtggccgc
     1261 gttgatcgcg gcctgcacct gctgctccgc gatgtcgagg gcgaggttca ggtcgaactg
     1321 cagcgtgatg acgctgctgg tggacgagct ggtcgacgtc atctgcttga ggccgggcat
     1381 ctggccgaac tggcgctcga gcggagcggt gaccgcggag gccatgacgt cggggctcgc
     1441 cccggggtag aaggtctgca cctgcatggt cgggtagtcg acctggggca gggcggagac
     1501 cgcgagcagc cggtaggcga cgaggccggc caggacgatc gcggcggtga ggagggaggt
     1561 ggcgaccggc cgtaggatga acggccgcga tggattcatg agcccgtgcg gccgggcggg
     1621 cgctccccgc gcgcgggtgg tcgctcgccc cgcgtggtgg gctccacgcg cgagcgctcc
     1681 tgcaccttgt cggccccgtc cacgatgagc gcgtcgcccg cggcgacgcc ctcgcgcacc
     1741 gcggtgaccc cgccctcggc ggggccgagc gcgaccttcc gcagctccac cgtcttgtcg
     1801 ggcttcacca cgtagacgaa ggcgccctgg gcgccgcgct ggatcgcctc gttgggcacc
     1861 agcaccgcgc ccttgagggt gtcgacgagc acgcgcgcat tgacgaactg gttcggatag
     1921 agcatgccgt cggcattctc gaaggcggcc ttgatcttga cggtgccggt gccctggtcg
     1981 atctggttgt cgaccgccac cagcgtgccg ctgccgatcc ggttgcgccc ctcgcgatcg
     2041 aaggcctcca ccgcgatggc gcccgccgcg cgctggcgcg cgagcaccga ctggaggttg
     2101 tcctggggga tcgtgaacag caccgcgatc ggctcgacct gggtgagcac cacgaggccg
     2161 cccgcgtcgt tggcgtgcac catgttgccc gcgtcgacga ggcgcagccc ggtccggccg
     2221 gtgatcgggg cgaggatgcg gcagtaggtg agctggaggc ggatggcgtc gatgttgccc
     2281 tggtcggcct tgatggacgc cgcgatctgc gaaacgctgg aggcctgggt gtcgagctgc
     2341 tgctgcggga tgaagccgcc cttgaccagg gtcttgtagc gctcgagatc gagacgcgcg
     2401 ttgctgagcg cggcctcgtc cttggcgagc tggccctcga cctgcgtgag ctgggcctcg
     2461 aagggccgcg ggtcgatctc ggccagcacg tcgccggcct tgacgatctg cccctcctgg
     2521 aaggaggtcc gcatgagctg gccgtcgacg cggctccgga ccgtcaccgt gttcagtgcg
     2581 gtgaccgagc cgaggcccgt caggtagacg ggcaggtcac cggtccgggc ggggacggtc
     2641 accaccggga ccgcccgggg gccggtggag gcggccggct gggcgcgccc gggacggttc
     2701 agggcgaagt agccgccgcc ggccaggagg cccgccagga cgatcccgat gacccagagc
     2761 cggcctgacg accggcgggg ggcgtccgag ggtgtcggac ggaactcccc ggactcatgc
     2821 gcttccccag ccataccggt ccccctctcg tgtggctccg cttgtagcat atttcgacgg
     2881 gccactccgc gggatcattc ggccggacgg gctcggaacg gcctccgggc cgggggcgac
     2941 cgggggccgg atcctccgca gccggtcacg aggtcgaccc gagcaggccg agccaggccg
     3001 ggggcggagc cccgagggcc cggagggtcc aggcgacgcg ctccggggtg cccagatcgc
     3061 accaggtgtg ggtcggcacc tcggccaccg ccaaggcgag ggacggcgac tcgaggaccg
     3121 tgcgcgagaa gtccgcggcc ggcgcgaaga gataggcctg tcggagggcc caggcctcgt
     3181 actgcgtgcc cgagaagagc cccatgcgca cgaggcggtc gtggaggagg ggcacgcact
     3241 cgcgacccgc ctcgatgagg gtggtcacgc tcgccgcgaa gacgaacgtg ttccagagtc
     3301 cgccgagcgc gtagaggcgc cgtgccgact cctcggtcgg cttctcccgg aagccgcgga
     3361 tgcggtggac ggggtccggc ccggcccagc ccagccggac cccgggctcg atccagctgt
     3421 agtcgggatc ggcgtcgctg ggcggcacgc cgagcaggac cagcccttcc ggatgcgcgc
     3481 gcacgtatcc ggccacctcg cccacgtggc gcatgaaggt ctcctcctcg aggatgaagt
     3541 ggtcggcggg gaacaccgcg accaccgccc gcggatcgcg cgcgcggatc cagtgggcgg
     3601 gcatcagcac gccggccgcg gtgccgcggt cggagggctg ggcgaggacg tgaatctgcg
     3661 ggaagccggc cagctccgcc ccgaggtagc ggtcgtggct ggcctgggcc acgaccacga
     3721 tgcgcgaggg cgggaccagc cgcgcgacgc gctccagggt ctgccgcagg agcgacttgg
     3781 agccggtcag gaccgcgtat tgcttgggcc gctcgtcgcc gtagagatag cgggtcagcg
     3841 cccggagccg gctgccctcc ccaccggcca gcacgacggc ccacaggtca ccgtcacggt
     3901 cgcgttcccg gatcgcggcg agcggctcgc tgcaagtgaa tgcctgctcg agcatggatc
     3961 tcctcctcgt tctcacctcc gttcgggttt cgacgcg
//
LOCUS       DATMCV010000855         3202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_112771, whole genome shotgun sequence.
ACCESSION   DATMCV010000855 DATMCV010000000
VERSION     DATMCV010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112771"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2521
                     /gene="glnE"
                     /locus_tag="VNU03_19665"
     CDS             <1..2521
                     /gene="glnE"
                     /locus_tag="VNU03_19665"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HWP76749.1"
                     /translation="TEELSHVADVCLAAAWEWTRERLERLYGAPLGADGAPTGLAVIG
                     MGKLGGLELNYSSDIDPIFVYGEDGETAGGESGRIANGEFFAQAVRGILETLESVTEE
                     GHVFRVDLRLRPEGRSGALILSVDGYRRYFADRAEPWERQALIKARACAGDPATAARF
                     LEAVRSFVYRPGLDPDIVGHVRGMKAQIDRSLRGKDAGRRNVKLGRGGIREVEFLVQA
                     LQLLYGGDDPWLRERNSLRAIFRLTERGYLPPALGRELGLALVYLRTVEHRLQIVHEF
                     QTHTLPEEPAALGLLARRMGIALPPAAAARRFVVEHRRVTTLVHRAFREFFAAPPAAA
                     AAPVRIPSYTALKATGFADPDRARQNLRRVLEGRPLVPYPAAAGRAIARLFPMLLDAL
                     WQSPDPDEALGQFERFVSAAGPRTAYLDLLADRPELLANLVRLCARGELLTELLVSQP
                     ELLNGLASPETFAAHKGERQFRAELAPALAPRLAAGERRDRLRRLKQAQELGIIWRML
                     LGVTDAERFSLEMTALAEAALAVAWTLALDETAALWGVPRDGDRGLIETAIIGLGKLG
                     GRELTTGSDLDLFVVYGGDGVTDGPETVEAHVFYDRAVEALSGLLGDITAAGIVFAVD
                     LRLRPGSKGSGFAAAADALERYYREWADPWERQTLTRARLVGGDPRLGRAVRRRIRGL
                     LYGPEAPPPDLKEMRALRERMERELGKETPGRFHVKFGRGGLVDVEFITQALQMRHGA
                     RHPGVRRANTLAAIRGIAAAELLPGSDCDLLIEQYRFLRRVSLGLRLFGTRPSDTLEV
                     AGPNPARLAKTLDYPSRKDFLDDYRRRAGWVRALYDRVVPR"
     gene            complement(2540..>3202)
                     /locus_tag="VNU03_19670"
     CDS             complement(2540..>3202)
                     /locus_tag="VNU03_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWP76750.1"
                     /translation="IGTNQLRIEGVAELHPGRHRLGPDHIEIGSFVALAAMTGGELHI
                     PDVVPDDLRMIRLAYRRLGVETALEGTTLIVPGPQELRVAADADGAIPKVDDAPWPGF
                     PADLTSLALVLATQSHGTVLIHEKLFESRLFFVDSLIAMGARIVLCDPHRAVVVGPSE
                     LRAAPIVSPDIRAGMALIGAALCARGTSVIMNAHQVDRGYERIDERLRGLGAHIKRDA
                     GA"
BASE COUNT          397 a         1198 c         1179 g          428 t
ORIGIN      
        1 caccgaggag ctctcccacg tggccgacgt gtgcctcgcc gcggcctggg agtggacccg
       61 cgagcggctc gagcggctct acggggcgcc cctcggcgcc gacggcgcgc ccaccgggct
      121 cgcggtgatc ggcatgggca agctgggcgg cctcgagctg aactactcct ccgacatcga
      181 cccgatcttc gtctacggcg aggacggcga gaccgcgggt ggggaatcgg gtcgcatcgc
      241 caacggggag ttcttcgccc aggcggtgcg cggcatcctg gagaccctcg agtcggtcac
      301 cgaggagggc cacgtcttcc gcgtcgacct gcgcctgcgc ccggagggcc gctccggggc
      361 gctcatcctc tcggtcgacg gctaccgccg ctacttcgcc gaccgggccg agccgtggga
      421 gcgccaggcc ctgatcaagg cgcgcgcctg cgcgggcgat cccgcgacgg ccgcgcgctt
      481 cctcgaggcg gtgcggtcat tcgtctaccg tcccggcctc gatccggaca tcgtggggca
      541 cgtgcgcggg atgaaggccc agatcgaccg ctcgctccgc ggcaaggacg cggggcgccg
      601 caacgtgaag ctcgggcgcg gaggcatccg cgaggtcgag ttcctggtgc aggcgctcca
      661 gctcctctac ggcggcgacg atccgtggct gcgcgagcgg aacagcctgc gggccatctt
      721 ccgtctcacc gagcgcggct acctgccgcc cgcgctcggc cgcgagctgg gcctcgcgct
      781 cgtgtatctc cggacggtcg agcaccgcct ccagatcgtg cacgagttcc agacccacac
      841 cctgccggag gagccggcgg cgctcggcct cctggcccgc cgcatgggca tcgcgctgcc
      901 gcccgcggcg gcggcccggc gcttcgtcgt cgagcaccgg cgcgtcacca cgctcgtgca
      961 ccgggccttc cgcgagttct tcgccgcgcc gcccgcggcc gccgcggcgc cggtgcgcat
     1021 cccgagctac accgcgctca aggccaccgg cttcgcggat ccggaccgcg cgcgccagaa
     1081 cctgcgccgg gtcctcgaag gccggccgct cgtcccgtat ccggccgccg cgggtcgcgc
     1141 gatcgcccgg ctcttcccga tgctgctcga cgcgctctgg cagagcccgg atcccgacga
     1201 ggcgctcggc cagttcgagc gcttcgtctc ggcggcgggc ccgcgcaccg cctacctgga
     1261 tctcctcgcg gaccggcccg agctgctcgc caacctggtg cgcctctgcg cccgcgggga
     1321 gctgctcacc gagctgctgg tgagccagcc cgagctgctc aacgggctcg cgagcccgga
     1381 gaccttcgcg gcgcacaagg gggagcgcca gttccgggcc gagctggccc cggcgctcgc
     1441 gccccggctc gccgcggggg agcgccgcga ccggctccgg cgactcaagc aggcacagga
     1501 gctgggcatc atctggcgga tgctgctggg ggtgaccgac gcggagcgct tctcgctcga
     1561 gatgaccgcc ctggccgagg ccgcactggc ggtggcgtgg acgctcgccc tcgacgagac
     1621 cgcggccctg tggggcgtgc cgcgcgacgg ggacaggggt ctgatcgaaa ccgccatcat
     1681 cggcctcggc aagctcggag gccgggagct gaccaccggc tccgatctcg acctcttcgt
     1741 ggtctacggc ggagacggcg tcaccgacgg gcccgagacg gtcgaggccc acgtcttcta
     1801 cgaccgcgcg gtggaggcgc tgagcggcct gctcggcgac atcaccgccg ccgggatcgt
     1861 gttcgcggtg gacctgcgcc tgcggcccgg ctcgaaaggc agcggcttcg ccgccgccgc
     1921 cgacgcgctc gagcgctact accgcgagtg ggccgacccg tgggagcgcc agacgctcac
     1981 ccgcgcccgg ctggtcggcg gcgacccgcg gctcggccgg gcggtgcggc gccgcatccg
     2041 cggcctgctc tacgggccgg aggcgccgcc ccccgacctc aaggagatgc gggccctccg
     2101 cgagcggatg gagcgcgagc tgggcaagga gacgccgggg cgcttccacg tgaagttcgg
     2161 gcggggcggc ctcgtcgacg tggagttcat cacccaggcc ctgcagatgc ggcatggggc
     2221 ccggcacccg ggcgtgcgcc gggccaacac cctggccgcc atccgcggga tcgcggcggc
     2281 cgagctcctg ccgggctccg actgcgatct cctgatcgag cagtaccgct tcctgcggcg
     2341 cgtctccctg ggcctgcgcc tcttcggcac gcgcccgtcc gacaccctgg aggtcgcggg
     2401 cccgaacccg gcgcggctcg ccaagacgct cgactatccc tcgcgcaagg acttcctcga
     2461 tgactaccgc cgtcgcgcgg gctgggtccg cgcgctctac gatcgcgtcg tgccgaggta
     2521 gtggcgggcg cggcccgggt caggcgcccg cgtcgcgctt gatgtgcgcg ccgagcccac
     2581 ggagacgctc gtcgatccgc tcgtagccgc gatccacctg gtgcgcgttc atgatcaccg
     2641 aggtgccccg cgcgcagagc gcggcgccga tcagggccat gccggcgcgg atgtcgggag
     2701 agacgatcgg ggcggcgcgt agctcgctcg gaccgaccac caccgcgcgg tgcggatcgc
     2761 acaggacgat ccgcgcgccc atcgcgatca ggctgtccac gaagaagagc cggctctcga
     2821 agagcttctc gtggatgagg acggtgccgt ggctctgggt ggccagcacc agcgcaagcg
     2881 aggtcaggtc ggcggggaag cccggccagg gcgcgtcgtc caccttgggg atggcgccgt
     2941 cggcgtcggc ggccacccgc agctcctgcg ggccgggcac gatcagggtc gtgccctcga
     3001 gcgcggtctc caccccgagg cggcggtagg ccagccggat catgcgcagg tcgtcgggca
     3061 ccacgtccgg gatgtggagc tcgccgccgg tcatggccgc gagcgccacg aaggagccga
     3121 tctcgatgtg atcggggccg agccggtggc gtccggggtg gagctcggcc accccctcga
     3181 tgcgcagctg gttggtaccg at
//
LOCUS       DATMCV010000856         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_512252, whole genome shotgun sequence.
ACCESSION   DATMCV010000856 DATMCV010000000
VERSION     DATMCV010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512252"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VNU03_19675"
     CDS             <1..384
                     /locus_tag="VNU03_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWP76751.1"
                     /translation="EPGDAVCVYSGREAWQAANPERPYGRPFGIGPNERPGLHVSCLE
                     FLRDHDVSVLVWDMLDHLPVGYDIPWAVHAALFAYGVALVDNALLEPLARACVEEQRD
                     EFMLIVAPLVVVGGTGSPANPLAVF"
     gene            complement(388..1467)
                     /locus_tag="VNU03_19680"
     CDS             complement(388..1467)
                     /locus_tag="VNU03_19680"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWP76752.1"
                     /translation="MKITAVDTAILRIPTRKPIALDFPHHSLVVAHVRTDEGVTGLGY
                     TLAFGGGGVESIQAYLVTRLAPLLIGQDPLFVERLWERMYRADRGIKRQGIAAYALSA
                     LDIALWDIAGKAAGLPLYKLWGAVTDRVAAYGSGGWPSYALEDLIGEAQAYVARGCRY
                     YKMKIHHPDPRANRARVEAVKKAVGDDVRLMVDVNQKLDVLGNIRQARLLADLDLVWY
                     EEPVLADDLAACAEVAARIDIPVATGENNYTRFEFRDLIERRAARYLMPDICRANGFS
                     ETLKIGRLAAAHQIAVSPHVVHELSLQVAGALSNSFLVEWIDWVPDDLFAQMPVMDHG
                     DFLIPDRPGHGMALAPGAERKYRID"
     gene            1732..>2223
                     /locus_tag="VNU03_19685"
     CDS             1732..>2223
                     /locus_tag="VNU03_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76753.1"
                     /translation="MALILLLIALPLYLWPLRGGGVGLPGAAALSGLPRDPRDATAVA
                     HLPADVWDGLMDEGRTGPKGDTRHVPGNLTRIARLEEVPGAGPLGIVSSGTSLSWPRD
                     TLPRPFTALNGDSPDQSTGTSDSPPAPVQFLSGPPGGEGTGSGSGWGGGTYSGLPNNL
                     GPFN"
BASE COUNT          306 a          769 c          794 g          354 t
ORIGIN      
        1 gagccgggcg acgcggtctg cgtctactcg ggtcgcgagg cctggcaggc cgcgaacccc
       61 gagcggccct acggccggcc cttcgggatc gggcccaacg agcgccccgg actgcacgtc
      121 tcctgcctcg agttcctgcg cgaccatgac gtgagcgtgc tggtgtggga catgctggat
      181 cacctgccgg tgggctacga catcccctgg gcggtgcacg ccgccctctt cgcctacggg
      241 gtcgccctcg tggacaacgc gctcctcgag ccgctcgcgc gggcgtgcgt ggaggagcag
      301 cgcgacgagt tcatgctgat cgtcgcgccg ctcgtggtgg tgggcgggac cggatccccc
      361 gcgaacccac tcgcggtgtt ttgagcgtca gtcgatccgg tacttccgct cggcccccgg
      421 ggcgagcgcc atgccgtggc cgggccggtc ggggatcagg aagtcgccgt ggtccatcac
      481 cggcatctgg gcgaagagat cgtcgggcac ccagtcgatc cactcgacga ggaagctgtt
      541 actgagcgca ccggccacct gcagcgagag ctcgtgcacc acgtggggtg agaccgcgat
      601 ctggtgggcg gcggcgagcc gtccgatctt gagcgtctcg ctgaagccgt tggcccggca
      661 gatgtcgggc atcaggtagc gggcggcgcg ccgctcgatc aggtcccgga actcgaagcg
      721 cgtgtagttg ttctctccgg tcgccaccgg gatgtcgatc cgggccgcca cctccgcgca
      781 ggcggcgagg tcgtcggcca gcaccggctc ctcgtaccag acgagatcga ggtcggcgag
      841 caggcgcgcc tgccggatgt tgccgagcac gtcgagcttc tggttgacgt cgaccatcag
      901 gcgcacgtcg tcgcccaccg ccttcttcac cgcctccacc cgcgcccggt tcgcacgcgg
      961 atccggatgg tggatcttca tcttgtagta gcggcagccg cgcgcgacgt aggcctgggc
     1021 ctcgccgatc aggtcctcga gcgcgtagct gggccagccg ccgctgccgt aggccgcgac
     1081 ccggtccgtc accgcgcccc acagcttgta gagcggcagg cccgcggcct tgcccgcgat
     1141 gtcccacagc gcgatgtcga gcgcgctcag cgcgtacgcc gcgatgccct gccgcttgat
     1201 gccgcggtcc gcgcgataca tccgctccca caggcgctcc acgaagagcg ggtcctggcc
     1261 gatcagcagc ggggcgaggc gcgtcaccag gtaggcctgg atggactcga cgccaccgcc
     1321 gccgaacgcg agcgtatagc cgagcccggt gacgccctcg tcggtgcgga cgtgcgccac
     1381 cacgaggctg tggtgcggga agtcgagcgc gatcggcttg cgagtgggaa tgcgcaggat
     1441 ggcggtgtcg acggcggtga ttttcatggc cgcatcctag ggcggccggg cgcagaagtg
     1501 caatggggcc ggtgggtagc cggttgccct gactgaggct accgccgtct caccccagaa
     1561 ggcggcttcc gcgcaattgc tgccgccggc cggcgtgtcg atgcaactgc gtgaactaga
     1621 tatgattttc ggcccaactc tgcatggcac gggcttcgct tagtcctcac gaacatgagg
     1681 cgctcgtcgg gcccagagtc tgccccggcg cgggggcgaa ggatcgcgat cgtggcgctg
     1741 atccttctgc tgatcgcgct gcctctctac ctctggccgc tgcggggagg cggtgtcgga
     1801 ctgcccggcg ccgccgcgct ctccggactg ccgcgcgacc cgcgggatgc gacggcggta
     1861 gcgcaccttc ccgccgacgt gtgggacggc ttgatggacg aggggcgaac gggcccgaag
     1921 ggtgacacga ggcatgtgcc cgggaacctc actcggattg cccggctcga ggaggtgccc
     1981 ggcgccgggc cgctcggcat cgtgtcgagc ggtacctcgc tgtcctggcc gcgcgacact
     2041 ctgccgcgcc cgttcactgc gctcaacggc gacagcccgg atcaatcgac cggcaccagc
     2101 gactcaccgc ccgcgcccgt gcagtttctc tcggggccac ctggaggcga gggcacgggc
     2161 tccgggagcg ggtggggtgg cggcacttac tcgggcttgc cgaacaacct cggcccgttc
     2221 aat
//
LOCUS       DATMCV010000857         4247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_580941, whole genome shotgun sequence.
ACCESSION   DATMCV010000857 DATMCV010000000
VERSION     DATMCV010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580941"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VNU03_19690"
     CDS             complement(<1..603)
                     /locus_tag="VNU03_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP76754.1"
                     /translation="MSGFADYERYDALGLADLVRRGKVTPTELLEAAIARVEARNPKV
                     NAVIMPLYDHGRRAIADGLPDGPFRGVPFLLKDLGAPLTGTTMTRGSRFFADTPPSTF
                     DSENVARLKRAGLVIFGRTNSCEVGLSLTCEPRLYGPTRNPWDPTRTSSGSSGGAAAA
                     VGARMLPMAHASDGFGSIRAPAASCGLVGLKPTRGRNTFAP"
     gene            complement(615..2255)
                     /gene="thiC"
                     /locus_tag="VNU03_19695"
     CDS             complement(615..2255)
                     /gene="thiC"
                     /locus_tag="VNU03_19695"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020375549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HWP76755.1"
                     /translation="MSNRKIYVSGSNPSVRVPVREVTLSNNERLRLYDTSGLYTDPDY
                     TPDVKQGLPPLRRHWVLSRADVEPGASGRWGLRAKPGHRVTQMHYARRGEITPEMEFV
                     ALREGMAPEVVRDEIARGRAIIPANINHPESEPMIIGRHFLVKINANIGNSAVSSSIE
                     EEVEKMTWATRWGGDTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVNGKAE
                     DLTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIMAKWCLAH
                     HRESFLYTHFREICEIMAAYDVAFSLGDGLRPGCQADANDDAQFAELDTLGELTTLAW
                     EQDCQVMIEGPGHVPMHLIKENMDRQLAVCHEAPFYTLGPLTTDIAPGYDHITSAIGA
                     AMIGWYGTAMLCYVTPKEHLGLPDRQDVKDGVIAYKIAAHAADLAKNHPKAREWDDAL
                     SRARFEFRWEDQFNLSLDPETARSFHDETLPADGAKIAHFCSMCGPHFCSMKITQDVR
                     DYAAKHGIGDETAAVVQGLRDKAAEFKKAGGQLYVKQP"
     regulatory      complement(2249..2359)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2476..>4247
                     /locus_tag="VNU03_19700"
     CDS             2476..>4247
                     /locus_tag="VNU03_19700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP76756.1"
                     /translation="MSDANPQELEVLAEASRLLTSTLDLAEVLDRLADIARTRLETDV
                     ARIWLLDEEGEVLRLAAHKGRVRSPLPAREQVATRSSLVGWVFTHRKPLALADVQQDR
                     RLEKRDWFKAEGFASLLCVPILLDGDVIGILSGMTRQRREFRAPDVALAEALTASAAV
                     AVRNARLHSETVRRLAEIEAFQRVAADTLSSPDVVTALEAVVREIPALLRSDGAACSR
                     VDPRTGALETVTTVGARGRAMQPMRLDPGHGLAGLVLREKRPLCTDDYFADTRFERPP
                     AVADWATAEGVVSMITVPVTDAAGEPIALLWAFNRSARPFTGRDEATLTGLARQAALA
                     MDNARLVGDLRRTLEELQAAQDTLVRGATLRAVGELAAGAAHHLNNLMAVVLGRSQLL
                     LYRNRDPEMGSALRTIERAALDAADTVRRIQAFSRAGHGASATVVDLNDTVRESVDLT
                     RARWEHEAQVRGAQIELVFEPGALPPVSGRNAELREVLTNLILNAVDALPRGGRLLIR
                     TWGEPGRALVSITDSGPGMPEDVRGRAFEPFFTTKGVRRLGLGLAVAYGLVSGHGGDI
                     ALESGEGRGTTVTFWIPAEARVEAG"
BASE COUNT          558 a         1482 c         1562 g          645 t
ORIGIN      
        1 cggcgcgaac gtgttgcggc ctcgcgtggg cttgagcccg accagcccgc agctcgcggc
       61 gggcgcgcgg atcgagccaa agccgtcgct ggcgtgcgcc atcgggagca tgcgggcgcc
      121 caccgcggcg gcggccccgc cgctcgagcc ggaggaggtg cgggtcgggt cccacgggtt
      181 gcgggtcggc ccgtagagcc gtggctcgca ggtgagcgag aggcccacct cgcagctgtt
      241 ggtgcggccg aagatcacga ggccggcgcg cttgagccgg gccacgttct cgctgtcgaa
      301 cgtggacggc ggggtgtcgg cgaagaaccg gctgccccgc gtcatggtcg tcccggtgag
      361 gggggcgccc aggtccttca agagaaacgg tacgccgcgg aacggcccgt cgggaagccc
      421 gtcggcgatc gcgcggcggc cgtggtcgta gagcggcatg atcaccgcgt tcaccttcgg
      481 gttacgcgct tccacccgcg cgatcgcggc ctcgagcagc tcggtgggcg tcaccttgcc
      541 gcgccgcacg aggtcggcca gcccgagggc gtcgtaccgc tcgtagtccg cgaagccgct
      601 catgtggatc cgcgctacgg ctgcttcacg tagagctggc cgcccgcctt cttgaactcg
      661 gccgccttgt cccgcaggcc ctgcaccacc gcggcggtct cgtcgccgat gccgtgcttg
      721 gccgcgtagt cgcgcacgtc ctgggtgatc ttcatcgagc agaagtgggg cccgcacatc
      781 gagcagaagt gggcgatctt ggccccgtcg gcggggaggg tctcgtcgtg gaaggagcgc
      841 gcggtctccg ggtcgagcga gaggttgaac tgatcctccc agcggaactc gaagcgggcg
      901 cgcgagagcg cgtcgtccca ctcgcgggcc ttgggatggt tcttggccag gtcggcggcg
      961 tgggccgcga tcttgtaggc gatgaccccg tccttgacgt cctgccgatc cgggagcccg
     1021 aggtgctcct tgggcgtgac gtagcagagc atcgcggtgc cgtaccagcc gatcatcgcc
     1081 gcgccgatcg cggaggtgat gtggtcgtag ccgggggcga tgtcggtggt gagcggcccc
     1141 agggtgtaga acggcgcctc gtggcagacc gcgagctgcc ggtccatgtt ctccttgatg
     1201 aggtgcatcg ggacgtggcc cggtccctcg atcatcacct ggcagtcctg ctcccacgcc
     1261 agcgtggtca gctcgcccag ggtgtcgagc tccgcgaact gggcgtcgtc gttggcgtcg
     1321 gcctggcagc cagggcgcag cccgtcgccc agcgagaacg ccacgtcgta ggcggccatg
     1381 atctcgcaga tctcgcggaa gtgggtgtag aggaagctct cccggtggtg ggccaggcac
     1441 cacttggcca tgatcgagcc gccgcgcgac acgatgccgg tgaggcgctt cgcggtgagc
     1501 ggaatgtagc gcagcagcac gcccgcgtgg atggtgaagt agtcgacgcc ctgctcggcc
     1561 tgctcgatga gggtgtcgcg gtagatctcc cacgtcaggt cctcggcctt gccgttgacc
     1621 ttctccagcg cctggtagat cggcaccgtg ccgaccggca ccggcgagtt gcgcaggatc
     1681 cactcgcggg tctcgtggat gttcttgccg gtggagagat ccatgatggt gtcgccgccc
     1741 cagcgggtgg cccacgtcat cttctcgacc tcctcctcga tggaggagga gaccgcggag
     1801 ttaccgatgt tggcgttgat cttcaccagg aagtgccggc cgatgatcat cggctcgctc
     1861 tccgggtggt tgatgttcgc cgggatgatc gcgcgccccc gggcgatctc gtcccgcacg
     1921 acctcggggg ccatgccctc ccgcagggcc acgaactcca tctcgggagt gatctcgccc
     1981 cgccgcgcgt agtgcatctg ggtcacccgg tggcccggct tggcgcgcag tccccagcgg
     2041 ccgctcgcgc ccggctccac gtcggcgcgg gagaggaccc agtgacgacg aagcggaggc
     2101 aggccctgct taacgtcggg cgtgtagtcg ggatcggtgt agagcccgct cgtgtcgtag
     2161 agccggaggc gctcgttgtt cgagagcgtg acctcgcgga ccggaacgcg caccgagggg
     2221 ttggagccgg acacatagat ctttcggttg gacatgcagg gcctcccttc gctggcatta
     2281 cccagttcag gtttgtagcg gtcggcagcg tccccgctgc cctctcagcc cggatcaccg
     2341 agctcccgcg tgggaatggc tgtccgaatg ctacgaccaa aggtaggggg ggtcaaggcc
     2401 gtccggacgg cgcgccgcgc gatggcgtgg cgccgggccc tgcgctatag tccacccccg
     2461 agcccgcccg atccgatgag cgacgcgaac ccgcaggagc tggaggttct cgccgaggcg
     2521 agccggctgc tcacctcgac gctcgatctc gccgaggtgc tcgatcgtct cgccgacatc
     2581 gcgcgcaccc ggctcgagac cgacgtggcg cggatctggc tgctcgacga ggagggcgag
     2641 gtgctgcggc tcgccgccca caagggccgt gtccgctcgc cgctgcccgc ccgggagcag
     2701 gtggccacgc gctcctccct ggtgggctgg gtgttcaccc accgcaagcc cctcgcgctg
     2761 gccgacgtgc agcaggaccg gcggctcgag aagcgggact ggttcaaggc cgagggcttc
     2821 gcctcgctcc tctgcgtgcc catcctgctc gacggagacg tgatcgggat cctctcgggc
     2881 atgacccggc agcgccgcga gtttcgcgcc cccgacgtgg cgctcgcgga ggccctgacc
     2941 gcgtccgcgg cggtggcggt ccgcaacgcg cgcctgcact cggagacggt gcgccggctg
     3001 gccgagatcg aggcgttcca gcgtgttgcc gccgacaccc tgtcctctcc ggacgtggtc
     3061 accgcgctcg aggcggtggt gcgcgagatc ccggcgctgc tgcgctccga cggggcggcc
     3121 tgctcgcggg tggacccgcg gaccggggcg ctcgagacgg tgaccaccgt cggcgcgcgc
     3181 gggcgcgcga tgcagccgat gcgcctcgat ccgggccacg ggctggccgg cctggtcctg
     3241 cgcgagaagc ggccgctctg caccgacgac tacttcgccg acacccggtt cgagcgcccg
     3301 cctgccgtcg cggactgggc caccgccgag ggcgtcgtga gcatgatcac ggtcccggtc
     3361 accgacgcgg cgggcgagcc gatcgcgctg ctgtgggcct tcaatcgcag cgcgcgcccg
     3421 ttcaccggtc gagacgaggc gaccctcacc gggctggccc gccaggccgc gctcgcgatg
     3481 gacaacgcgc gcctggtcgg cgatctgcgc cgcaccctgg aagagctgca ggcggcccag
     3541 gacacgctcg tgcgcggggc gacgctccgc gcggtggggg agctggccgc gggggccgcg
     3601 caccacctca acaacctcat ggcggtcgtg ctggggcgaa gccagctcct gctctatcgc
     3661 aaccgcgatc ccgagatggg ctccgccctc cgcaccatcg agcgcgccgc cctcgacgcg
     3721 gccgacaccg tccggcgcat ccaggccttc agccgggccg ggcacggagc gagcgcgacc
     3781 gtcgtggatc tgaacgacac ggtgcgggag agcgtcgacc tcacgcgcgc gcgctgggag
     3841 cacgaggcgc aggtgcgcgg cgcgcagatc gagctcgtgt tcgagcccgg cgccctgccc
     3901 ccggtctcgg ggcgcaacgc ggagctccgc gaggtgctca ccaacctgat cctgaacgcg
     3961 gtggacgcgc tcccgcgggg gggacgcctc ctgatccgca cctggggcga gccggggcgg
     4021 gcgctcgtct cgatcacgga ctctggcccc ggcatgcccg aggacgtgcg cggccgcgcc
     4081 ttcgagccgt tcttcaccac caagggcgtg cggcggctcg ggctcgggct ggcggtcgcc
     4141 tacggcctcg tcagcggcca cgggggcgac atcgcgctcg agagcggcga gggccgcggc
     4201 accaccgtga ccttctggat tcccgccgag gcccgcgtcg aggccgg
//
LOCUS       DATMCV010000858         3452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_383235, whole genome shotgun sequence.
ACCESSION   DATMCV010000858 DATMCV010000000
VERSION     DATMCV010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383235"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VNU03_19705"
     CDS             complement(<1..384)
                     /locus_tag="VNU03_19705"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWP76757.1"
                     /translation="MTCTACGQANPGAAQFCGGCGARLAAVCPSCQAANPPDNRFCHQ
                     CGATLGSRPAPAVIVAPTPAPRSYTPRHLAEKILTTGAALKGERKQVTVLFVDVSGFT
                     SLSERLDPEEVHRLMSRAFDLMLAEV"
     gene            complement(398..1366)
                     /locus_tag="VNU03_19710"
     CDS             complement(398..1366)
                     /locus_tag="VNU03_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP76758.1"
                     /translation="MLMRKLGRTGLKVAALCLGGNTFGWTTDQKASEAVLDAYVEAGG
                     NFIDTADVYSRWAPGNTGGESEHALGLWLAARKHRPSVLIATKVCGPMGPGPNDKGLS
                     RQHIMEGVEASLRRLQTDYIDLYQAHWDDLETPLDETLRAFDDLVRQGKVRYIGASNY
                     PAWRLTRALWESDTNGTVRYECLQPKYNLVIRDEYERELEPLCLEQEVGVIPYSSLAS
                     GFLSGKYRAGEALPKTARAGGVEKTYMNDRGFRVLAAVEKVAAAASATAAQVSLSWLA
                     HRPGITAPIASATTVPQLKELVGGIELRLDAEATAALDRASAWRES"
     gene            complement(1397..2407)
                     /locus_tag="VNU03_19715"
     CDS             complement(1397..2407)
                     /locus_tag="VNU03_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76759.1"
                     /translation="MKSTTSSTIPAFLSVDVEPDGFQLDRRDPPGWGGYVAMVDYVTR
                     LREDLAGWTGRTPRFGWYVRTDPQIAEVHGRPDHALVAFPGCVDRLRAAGDYFGVHSH
                     LIRWAPERGEWVHEFSDDGWQAHATRFALDAFGQWAGAPSRLFRSGAGFLSDRVLEVV
                     EQAGVAIDLSLEPVTGRGVGAAAALTAVDSAPIVGVYPDCAGAPRVPYRPARHDFRVD
                     GGRAGRRLLMVPLTTRSLVPPRPRWRRAARRVLRGPAPTTVEVLYPTAPWPSAHGYWD
                     VVDQQLRSMPRPYLSLGIRTDAVGSETAARVRRIFDALPGHPLAQRLRFENPLITFGD
                     AL"
     gene            2512..>3452
                     /locus_tag="VNU03_19720"
     CDS             2512..>3452
                     /locus_tag="VNU03_19720"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76760.1"
                     /translation="MTRAYHIIDADGHVLEPVDLWDKYIDPAYRDRAPRMIIDTDGKE
                     RLLVEGQVLGSKKGFNVVGAIGSRQGGVPEATMKYVDGRAGGFDPHARIPDMDLDGID
                     AAFLYPSVGLFAGAVREPALAGAMCRAYNRWLADYCHPYPDRLFGVAMLPMQSIELAI
                     EEMRFARRELGMRGGFLRPNPYNGRTLHDPAYAPFWEEAQDLDFAIGLHEGASGGMPQ
                     VGVDRFETRGAKHIISHTMEMMLAAMSVIWGGVCDRYPRLRIAFLESGGGWIAPWLDR
                     MDRHFDDQGFNDSGLTTRPSDLFQRNCWISFEPVEGSL"
BASE COUNT          497 a         1235 c         1236 g          484 t
ORIGIN      
        1 cacctccgcc agcatgaggt cgaaggcgcg gctcatgagc cggtggacct cttcgggatc
       61 gagccgctcg gacagcgacg tgaagcccga cacgtcgacg aacagcacgg tgacctgctt
      121 gcgctcgccc ttcagcgcgg ccccggtggt gaggatcttc tcggccaggt gccggggcgt
      181 gtaggagcgg ggcgccgggg tcggggcgac gatgacggcg ggggcgggtc tcgatccgag
      241 cgtcgcgccg cactggtggc agaagcgatt gtccggcggg ttcgccgcct ggcaggaagg
      301 gcagaccgcg gccaggcgcg ccccgcagcc gccacagaac tgcgcggccc cgggattcgc
      361 ctgaccgcag gcggtacagg tcatgcgggt cgcgcctcta gctctcccgc caggcgctcg
      421 cccggtcgag agcagcggtg gcctcggcgt ccagccggag ctcgatgccg ccgaccagct
      481 ccttgagctg cggcaccgtg gtcgcgctgg cgatcggcgc ggtgatgccg ggccggtggg
      541 cgagccacga cagcgacacc tgcgccgcgg tcgcgctcgc ggccgcggcc accttctcca
      601 ccgcggccag cacccggaag ccgcggtcgt tcatgtaggt cttctccacc ccgcccgcgc
      661 gcgcggtctt gggcaatgcc tcgcccgccc gatacttgcc cgacaggaag ccgctcgcga
      721 gcgaggagta cgggatcact cccacctcct gctcgaggca gagcggctcc agctcgcgct
      781 cgtactcgtc gcggatcacc aggttgtact tgggctgcag gcactcgtag cgcacggtgc
      841 cgttggtgtc gctctcccag agcgcacggg tgagccgcca cgccgggtag ttggaggcgc
      901 cgatgtagcg caccttgccc tgccgaacca ggtcgtcgaa ggcgcggagc gtctcgtcga
      961 gcggcgtctc cagatcgtcc cagtgagcct ggtagagatc gatgtagtcg gtctggagcc
     1021 gccgcagcga ggcctcgacc ccctccatga tgtgctgccg cgagagaccc ttgtcgttcg
     1081 gccccggccc catggggccg cagaccttgg tcgcgatcag caccgagggg cggtgcttgc
     1141 gcgcggcgag ccagaggccg agcgcgtgct ccgactcgcc gcccgtgttg ccgggcgccc
     1201 agcgcgagta cacgtcggcg gtgtcgatga agttgccgcc cgcctcgacg taggcgtcga
     1261 gcaccgcctc cgaggccttc tggtcggtgg tccagccgaa cgtgttgccg cccaggcaca
     1321 gcgcggccac cttgagcccg gtgcgtccca gcttgcgcat cagcatgatc ggtcgtcctc
     1381 ctggagtcgc gtcgcgctag agcgcgtcgc caaaggtgat gagcgggttc tcgaagcgca
     1441 gccgctgcgc gagcggatgc cccggcaggg catcgaagat ccggcggacc ctggccgcgg
     1501 tctccgaccc gaccgcgtcg gtgcggatcc cgagcgagag atagggtcgc ggcatcgacc
     1561 ggagctgctg gtcgacgacg tcccagtacc cgtgcgcgct cggccagggg gccgtgggat
     1621 agagtacctc cacggtggtc ggggcggggc cgcgcagcac gcgccgcgcc gcgcgccgcc
     1681 atcgcggccg cggcggcacg agcgagcgcg tggtcagcgg caccatgagg agccggcggc
     1741 ccgcgcggcc cccgtcgacc cggaagtcgt ggcgcgcggg ccggtacggc acgcgcggcg
     1801 cgcccgcgca gtcggggtag acgcccacga tgggcgcgga gtccaccgcc gtcagagccg
     1861 ccgcggcgcc cacgccccgg ccggtcaccg gctcgagcga gaggtcgatc gcgacgccgg
     1921 cctgctcgac cacctccaaa actcgatcgc tcaggaaccc ggcgcccgac cggaagagcc
     1981 gggacggagc cccggcccac tggccgaagg cgtcgagggc gaaccgggtg gcgtgggcct
     2041 gccagccgtc gtcgctgaac tcgtgcaccc actcgccgcg ctccggggcc cagcggatca
     2101 ggtgcgagtg caccccgaag tagtcgcccg cggcccgcag ccggtcgacg caccccggga
     2161 acgccaccag cgcgtggtcg gggcgcccgt gcacctcggc gatctgggga tcggtccgca
     2221 cgtaccagcc gaagcgtggg gtgcgcccgg tccagccggc caggtcctct cgcagccgcg
     2281 tgacatagtc gaccatcgcc acgtagccgc cccagcccgg ggggtcccgg cggtccagct
     2341 ggaacccatc cggctcgacg tcgacactca ggaaggcggg gatggtgctc gacgtcgtcg
     2401 acttcatcgg tagagccgca tctcacaata cccgccaccc tctgtcaagg atggtgcgga
     2461 cggccgcgcg cgctgctaga gtctcctcga gattccgaaa ggaggccctc catgacccgc
     2521 gcctatcaca tcatcgacgc cgacggtcac gttctcgagc cggtggatct ctgggacaag
     2581 tacatcgacc ccgcctaccg cgaccgcgcc ccccggatga tcatcgacac cgacggcaag
     2641 gagcggctgc tcgtcgaggg gcaggtgctc ggcagcaaga agggcttcaa cgtcgtcggc
     2701 gccatcgggt cccggcaggg cggggttccc gaggccacga tgaagtacgt ggacggacgc
     2761 gcgggcggct tcgatccgca cgcacgcatc ccggacatgg atctggacgg catcgacgcg
     2821 gccttcctct acccgagcgt gggcctgttc gcgggcgcgg tccgggagcc cgcgctcgcg
     2881 ggcgcgatgt gccgcgccta caatcgctgg ctcgccgact actgccatcc ctaccccgac
     2941 cgcctgttcg gggtggcgat gctgccgatg cagtcgatcg agctcgccat cgaggagatg
     3001 cgcttcgccc gccgggagct gggcatgcgc ggcgggttcc tgcgccccaa tccctacaac
     3061 gggcgcacgc tgcacgaccc cgcctacgcg ccgttctggg aggaggcgca ggacctcgac
     3121 ttcgcgatcg gcctgcacga gggcgccagc ggcgggatgc cgcaggtggg ggtggaccgc
     3181 ttcgagacgc gcggggccaa gcacatcatc tcccacacca tggagatgat gctggccgcg
     3241 atgagcgtga tctggggcgg agtgtgcgac cgctacccgc gcctccgcat cgcgttcctc
     3301 gagtcgggcg gcgggtggat cgcgccgtgg ctcgaccgca tggaccgcca cttcgacgat
     3361 caggggttca acgattccgg cctgacgact cggccgagcg acctgtttca gcgcaattgc
     3421 tggatctcgt tcgagccggt cgagggcagc ct
//
LOCUS       DATMCV010000859         5053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_842352, whole genome shotgun sequence.
ACCESSION   DATMCV010000859 DATMCV010000000
VERSION     DATMCV010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842352"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1570)
                     /locus_tag="VNU03_19725"
     CDS             complement(35..1570)
                     /locus_tag="VNU03_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP76761.1"
                     /translation="MRAGPGVRWWAPGDWNAFCGLALDNLSLFVILSGLLIGVFGFPS
                     DLVLRVMVPGTALGVLLGDLAYTWLAVRLMRRTGRSDVTAMPFGIDTPSLFGMVFGVL
                     GPAMLLTRDPILAWKIGMGVTVAMGVIKLGLAFAGDWVRRLVPRAALLGSIAGVAVLL
                     IAFLPALRIFADPLVGVVSLTVVLVALVGGRRLPWGIPGALAAVLAGTAIFWARWALT
                     GAGGAEARPVLALGTLGLALPMPTLAWLGTVDALLPYLPLAVPFALATVVGGIDNTES
                     AIVAGDEYRTRDILLTEALATIAAGCCGGVVQNTPYIGHPAYKAMGARAGYTLATGLL
                     IGVGAAIGVVALLVHLLPDAAVAPILVFVGLQITGQAFLASPARHAPAVALAFLPAIA
                     ALVLIQGNLLLGGVGKSAADLAGEGRAMWSTLLVLGNGFILTALLWGSALVAIIEKRL
                     PVAGAVLAIAAVATLFGVIHSPLPDGAVFWPWAIASGAPHTLAAAYGVAGALLVLLGR
                     GDF"
     gene            complement(1575..1892)
                     /locus_tag="VNU03_19730"
     CDS             complement(1575..1892)
                     /locus_tag="VNU03_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76762.1"
                     /translation="MFSGGEYEEVARWLRNFVASHAKRENLRAEPVVDTEGPREGQSF
                     GVRLRLGAGLYPAPPAPPLELGFPEVAQNRGSLAWCAGLAARVRALARELPAAGSGPL
                     RTA"
     gene            complement(1902..4178)
                     /locus_tag="VNU03_19735"
     CDS             complement(1902..4178)
                     /locus_tag="VNU03_19735"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWP76763.1"
                     /translation="MPSITRPPKMGRWSEPALRVLRERYLVREQGQVRETPEEMCWRV
                     ALAIASAETRFGRTPAGVSEVATAFYDMMVDGYFLPNSPTLMNAGKDNALQYSACYVL
                     PVGDSMEEIFESVKAAAIIQQSGGGTGFAFSRLRPKDDVVHSTGGRASGPVSFLRVFN
                     AATEAVKQGGARRGANMGILRVDHPDLLEFIECKLDGGITNFNISVAATDTFMEALAA
                     DGEYDLINPRTGQVTGRLRARDVFARIVRAAWRTGDPGMVFIDRINRSPANPTPDLGL
                     IEATNPCGEQPLLPNDACNLGSINVAKFAVERSGRWEIDWTEMERVVRLAVRFLDDVI
                     EVNPYPLPQVDEMVKANRRVGLGIMGWADLLFTLGVPYDSDEALELAERLMAFVNERG
                     HDQSARLAEERGPFPHWARSIYRHGRPVRNATVTTIAPTGTISMIAGCSSGIEPVFAL
                     AFEHRVKGPDGERVLPFFSETFERLAHRRGFHSESLMTEVARRGSLRDVPGVPEEARE
                     IFKTAHEIDAIWHVRHQAAFQKHTDNGVSKTINLPENATEEDVAAAYRLAWDLGCLGI
                     TVFRDGSKGSQVLNVGLRPDAAAGEPPARGVKPRPHSLTGATYRMETPIGTAFITVND
                     NGGGEPFEVFVQVGKAGSDTMAVAEALGRLVSLVLRLPSPLTPTRRLEEAISQLLRIG
                     GGQPTGFGAAKVLSLPDALARTLAEHIGIPRAAAPVEPPAVHPLRRVGDLCRECGQAT
                     LVYEEGCKKCLSCGFNEC"
     gene            4426..>5053
                     /locus_tag="VNU03_19740"
     CDS             4426..>5053
                     /locus_tag="VNU03_19740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWP76764.1"
                     /translation="MMREQFALSMALVLTLGILPPAVRAQTPPPATTLPPAAPPPAVM
                     QTPPSMPPREPLPPPPGIRPFATPQELVGRELGLEEAVNIGLENAPLIVARIGDYIAA
                     QQRVNQALAPLLPQLSGSGNFGRQRSLSTFGGDAGYNDFGSASITASQLLFDFGRTWA
                     AKDAAKYTAVAIKESLETQKLDISQLVKTQYFTLLLSKRLVQVNLAALD"
BASE COUNT          765 a         1869 c         1763 g          656 t
ORIGIN      
        1 cgagcgaccg cgagatgctc gaccaggtcc gcggctagaa atccccgcgg ccgagcagca
       61 ccagcagcgc ccccgccacc ccgtacgccg cggccagcgt gtgcggcgcg ccgctcgcga
      121 tggcccaggg ccagaacacc gcgccgtcgg ggagtggcga gtggatcacg ccgaagagcg
      181 tcgccaccgc cgcgatcgcg agcaccgcgc ccgccacggg gagccgcttc tcgatgatcg
      241 cgaccagggc ggagccccac agcagcgcgg tgaggatgaa gccgttgccg agcacgagga
      301 gggtgctcca catcgcgcgg ccctcgcccg ccagatccgc cgcgctcttg ccgacccctc
      361 ccagcagcag gttcccctgg atgagcacga gcgccgcaat cgcgggcagg aaggcgaggg
      421 ccaccgcggg cgcgtgccgc gcgggggaag cgaggaaggc ctggccggtg atctgcaggc
      481 ccacgaagac caggatgggc gcgaccgccg cgtcgggcag caggtgcacc agcagcgcga
      541 cgaccccgat cgcggcgccg acgccgatga gcagcccggt ggcgagcgtg tagcccgcgc
      601 gcgcgcccat cgccttgtag gcgggatggc cgatgtacgg ggtgttctgg accaccccgc
      661 cgcagcaccc ggccgcgatg gtggcgaggg cctcggtgag caggatgtca cgcgtgcggt
      721 actcgtcgcc cgccacgatc gccgactcgg tgttgtcgat gccgcccacc acggtggcga
      781 gcgcgaacgg caccgcgagc ggcaggtacg gcagcagggc gtcgacggtg ccgagccacg
      841 cgagcgtggg catcggcagc gcgagcccga gcgtccccag cgccagcacc gggcgcgcct
      901 ccgcgccccc ggccccggtc agagcccacc gggcccagaa gatcgcggtg cccgcgagca
      961 ccgcggcgag cgcgcccggg attccccacg gcagccggcg accacccacc agggccacca
     1021 gcacgacggt gagcgagacc acgcccacca gcgggtcggc gaagatccgc agcgcgggca
     1081 ggaaggcgat gaggagcacc gccacccccg cgatcgagcc gagcagcgcg gcgcgcggca
     1141 cgaggcgacg cacccagtca cccgcgaacg cgagcccgag cttgatcacc cccatcgcca
     1201 ccgtcacgcc catgccgatc ttccaggcga ggatcgggtc gcgggtgagg agcatcgcgg
     1261 gccccagcac gccgaagacc atgccgaaga gcgagggggt gtcgatgccg aagggcatcg
     1321 cggtgacgtc gctccgcccc gtgcgccgca tgagccgcac cgcgagccac gtgtaggcca
     1381 gatcgcccag cagcactccg agcgcggtgc cggggaccat cacgcgcagc accaggtcgg
     1441 agggaaagcc gaagacgccg atcaggaggc cggacaggat gacgaacagg ctcaggttgt
     1501 cgagcgcgag cccgcagaac gcgttccagt cgccgggagc ccaccagcgg acgcccggcc
     1561 ccgccctcac cgggtcaagc ggtgcggagc gggcccgacc cggccgcggg cagctcgcgc
     1621 gcgagtgcgc gcacgcgcgc cgcgaggccg gcgcaccacg cgaggctccc ccggttctgc
     1681 gccacctccg ggaagccgag ctcgagcgga ggcgcgggcg gcgcgggata gagcccggcc
     1741 cccagccgga gacgcacccc gaagctctgc ccctcgcgcg ggccctcggt gtccaccacc
     1801 ggctcggcgc gcaggttctc ccgcttggcg tgcgaggcca cgaagttccg cagccaccgc
     1861 gccacttcct cgtactcacc tccggagaac atcggcgccc cctagcactc gttgaagccg
     1921 caggagagac acttcttgca gccctcctcg tacacgaggg tggcctggcc gcactcgcgg
     1981 cagagatcgc ccacccggcg cagcgggtgc accgccggcg gctcgaccgg ggccgcggcc
     2041 cgcgggatcc cgatgtgctc ggcgagggta cgggccagcg cgtcgggcag cgacagcacc
     2101 ttggccgcgc cgaagccggt cggctgccca cccccgatgc gcaggagctg gctgatcgcc
     2161 tcctcgagcc gccgggtcgg ggtgagcggc gagggcaggc gcaggacgag cgacacgaga
     2221 cgcccgagcg cctccgcgac cgccatggtg tcggagcccg ccttgccgac ctgcacgaag
     2281 acctcgaagg gctcgccgcc gccgttgtcg ttcacggtga tgaatgcggt gccgatcggc
     2341 gtctccatgc ggtaggtggc cccggtgagg ctgtgggggc gcggcttcac cccgcgggcg
     2401 ggcggctcgc ccgccgccgc gtcgggccgg agccccacgt tcagcacctg gctgcccttg
     2461 gagccgtcgc ggaacacggt gatgccgaga cagccgaggt cccaggcgag gcggtaggcg
     2521 gcggccacgt cctcctcggt cgcgttctcg ggcaggttga tcgtcttcga gactccgttg
     2581 tcggtgtgct tctggaacgc ggcctggtga cgcacgtgcc agatcgcgtc gatctcgtgt
     2641 gcggtcttga agatctcgcg ggcctcctcg ggcacgccgg gcacgtcgcg cagcgagccg
     2701 cggcgcgcca cctcggtcat cagcgactcc gagtggaagc cgcggcgatg ggcgagccgc
     2761 tcgaacgtct cgctgaagaa cggcagcacc cgctcaccat ccggcccctt cacccggtgc
     2821 tcgaaggcga gcgcgaagac cggctcgatc cccgacgagc agcccgcgat catcgagatg
     2881 gtcccggtcg gcgcgatggt ggtcacggtg gcgttgcgga cgggccggcc gtgtcggtag
     2941 atcgagcggg cccagtgcgg gaagggtccc cgctcctccg cgagccgcgc ggactggtcg
     3001 tggcctcgct cgttgacgaa ggccatgagc cgctccgcca gctcgagggc ctcgtcgctg
     3061 tcgtagggca ccccgagggt gaagagcagg tcggcccagc ccatgatccc gaggcccacg
     3121 cgccggttgg ccttcaccat ctcgtcgacc tgcggcagcg ggtacgggtt cacctcgatg
     3181 acgtcgtcga ggaaccgtac cgccaggcgc accacgcgct ccatctcggt ccagtcgatc
     3241 tcccaccgcc ccgatcgctc gaccgcgaac ttggccacgt tgatcgagcc caggttgcac
     3301 gcgtcgttcg gcaagagtgg ctgctcgccg catgggttag tcgcctcgat cagcccgagg
     3361 tccggggtgg gattcgccgg gctccggttg atgcggtcga tgaagaccat gccgggatcg
     3421 ccggtgcgcc acgccgcgcg tacgatgcgc gcgaagacgt cgcgcgcgcg cagccggccg
     3481 gtgacctgcc cggtgcgcgg gttgatcagg tcgtactccc cgtccgcggc gagcgcctcc
     3541 atgaacgtgt cggtggcggc caccgagatg ttgaagttgg tgatgccgcc gtcgagcttg
     3601 cattcgatga actcgagcag gtccgggtgg tcgacgcgga ggatccccat gttggccccg
     3661 cgccgcgccc cgccctgctt gaccgcctcg gtggccgcgt tgaacacgcg caggaaggac
     3721 accggtcccg acgcgcgccc gcccgtcgag tgcaccacgt cgtccttggg gcggaggcgc
     3781 gagaacgcga agccggtgcc gccgcccgac tgctggatga tcgcggccgc cttcaccgac
     3841 tcgaagatct cctccatgga gtcgcccacc ggcaggacgt agcaggccga gtactgcagc
     3901 gcgttgtcct tgcccgcgtt catgagcgtc ggggagttcg gcaggaagta gccgtcgacc
     3961 atcatgtcgt agaaggcggt cgccacctcg gagaccccgg ccggggtgcg cccgaagcgg
     4021 gtctccgcgg aggcgatggc cagcgccacc cgccagcaca tctcctcggg ggtttcgcgc
     4081 acttgcccct gctcccggac caggtagcgc tcgcgcagga cgcgcagggc gggctccgac
     4141 cagcggccca tcttgggcgg tcgggtgatg gacggcatgg ctctcgctta cccctcccct
     4201 ggtatccggc gcccgagacc ctccccgcgc ctggggtacc ctccggacta tctcacaaac
     4261 ttgactacag tccaaaggtc cctacaattg ggcggaattg acttctccac cgagatttcc
     4321 ccatgcgcct gcagcgggtc cgggcgctcc ccctccgaat acgaggctcc gtctgacgtc
     4381 ttctatgggc accgctgttc ctcacgcttc gtcaggagat ccggcatgat gcgggagcaa
     4441 tttgcgctgt ccatggcgct ggtcctcacc ctggggatac tgccgcccgc ggtccgggcg
     4501 cagacacccc ctccggcgac cacgctgccg ccggccgccc cgccgccggc ggttatgcag
     4561 acgcccccgt cgatgccgcc gcgcgagccc ctgccgccgc cgcccggaat ccggccgttc
     4621 gccaccccgc aggagctggt ggggcgcgag ctggggctag aggaagcggt caatatcggg
     4681 ctcgagaacg ccccgctgat cgtggcgcgc atcggcgatt acatcgcggc ccagcagcgc
     4741 gtgaaccagg ccctcgcccc cctcctgccc cagctctccg gctccggcaa cttcggccgg
     4801 cagcgctcgc tcagcacctt cggcggcgac gccggctaca acgacttcgg gtcggcgagc
     4861 atcaccgcct cccagctcct gttcgacttc gggcggacct gggccgccaa ggacgcggcc
     4921 aagtacaccg cggtggcgat caaggaatcg ctcgagacac agaaactcga catctcccag
     4981 ctcgtgaaga cgcagtactt cacgctgctg ctctccaagc gcctggtcca ggtgaacctg
     5041 gccgcgctcg atc
//
LOCUS       DATMCV010000860         3080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_981394, whole genome shotgun sequence.
ACCESSION   DATMCV010000860 DATMCV010000000
VERSION     DATMCV010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981394"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            524..1744
                     /locus_tag="VNU03_19745"
     CDS             524..1744
                     /locus_tag="VNU03_19745"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWP76765.1"
                     /translation="MLPNGMRLIIQEHRAASTVAIHLWTGVGGRDEAAGERGFSHFAE
                     HMLFKGTATRPRGFVESEVESVGGRTNAGTSNDYTFYYLLLPAARALPGIALIADMVL
                     NSVFDPAELTREREVVFEEVRLNEDNPRSSLGRQLYTLLYQEHPYGRPVLGDAADLRA
                     ATQETLRGYYARYYVPNNMTLVVVGPVDPAAVRAAALEAFGARPAAPYTRPPLPAPGR
                     FDGVRDRAVERPERQTQLGLAWLGPPLGDPDMAAVDVLGHILGGSRSSRLNQALRERT
                     RLVSSINGWYGALQGAGAIGVTAQLEGRDAEAVERAILAEVERVRSEGVTPDELARAI
                     TASEAEREFSRETVEGLALAYGRAETTWNLEEERAYVDRVRKVTRADVQAAARRYLTP
                     SYARLTFVPRARTP"
     gene            1741..3048
                     /locus_tag="VNU03_19750"
     CDS             1741..3048
                     /locus_tag="VNU03_19750"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWP76766.1"
                     /translation="MRTSRLVSTAVLAATAAWALAGVAAAQGPRREQLPSGLTVLVRE
                     NTATPVVAASLFVRVGSRWETEADAGITHLLQQVLLKGTLTRSALEIAETAEGLGGGI
                     SASADMDFSELRATALARNWKKMIELMADVALRPTLPDAEIDGERRAMLTALRSRQDQ
                     PFPLAMDTVMSRVYGDHPYGRPVLGRPAALERVDRAALLAYHQRFYRAPRMILAVSGD
                     VSAREVMAEVTRRFATAPTGEGDPEPTLPAAAARADRTVLVRPSAQAQVLVAFLAPPT
                     SHPDYAAVKVLATALGGGMAGRLFTEVRDKQGLAYSTGGAYPSRLGPGVLYMQLGTAP
                     ANQVRAEAAMLGELERIRRDPVNPAELTRAKAYLLGQFALDRRTNARLAWYDAFFEVL
                     GVGLDFSDRYARAVEAVSAEGVLRVARTYLAAPTIVTLGPAAQ"
BASE COUNT          430 a         1130 c         1079 g          441 t
ORIGIN      
        1 cacctcaggg gggcaggagg tctgcgccat cccggccgtg gccgagagtg agaccatcgt
       61 gaccacgctc aacacactcg cgagaacctt tttcattgaa tccatccttt ctgactgcgt
      121 tttcacgtct gacctcgttt ccaccttggc tccacctctt ggccccctgg ggccccgact
      181 cgcgccctta ggatgcagag ccgatgccat tgggagcagc ctctaaggat cttagtctta
      241 tcaagtgttt acggttcgtt gtcccctcag gctccggtgc ccacgagggc gttctgcccg
      301 gcatgccgct gatgggggga tcgacaatct gcctcggaag agactccctt gaggcctcat
      361 catcgctctg ataccgtgga gcgccatgac ccccaccccc agcagacgca gaagcgtctg
      421 cgcgctcgcg atcgtcctcc tcacgacagc cggatgcgcc accggcaccg catcgtccgc
      481 tccatcctcg ccatcgcacg gtctgccggc tccgatccgc gaggtgctcc cgaacgggat
      541 gcggctgatc atccaggagc accgcgccgc cagcaccgtg gcgattcatc tctggaccgg
      601 ggtcggggga cgtgacgagg cggcgggcga gcgcggcttc tctcacttcg cggagcacat
      661 gctgttcaag gggaccgcca cgcgcccgcg cggcttcgtc gaatccgagg tcgagtcggt
      721 gggcggacgc accaacgcag ggacctccaa cgactatacc ttctattatc tcctgctacc
      781 cgcggcccgc gcgctgccgg gcatcgccct gatcgcggac atggtgctca actccgtctt
      841 cgatcccgcc gagctcacgc gggagcgcga ggtggtgttc gaggaggtgc ggctgaacga
      901 ggacaacccg cgctcgtcgc tgggacgcca gctctacacc ctgctgtacc aggagcaccc
      961 gtacggacgg ccggtgctgg gtgacgccgc cgacctgcgg gccgcgaccc aggagaccct
     1021 ccgcggctat tacgcgcgct actacgtccc gaacaacatg acgctggtgg tggtcggccc
     1081 cgtcgatccg gccgcggtgc gagcggccgc gctggaggca ttcggcgccc ggccggccgc
     1141 gccgtacacg cgcccgccgc tccccgcccc cggccgattc gacggcgtcc gcgaccgggc
     1201 ggtggagcgg cccgagcgac agacccagct cgggctcgcg tggctcgggc cgccgctcgg
     1261 ggaccccgac atggccgcgg tggacgtgct cggccacatc ctgggcggct cgcgcagctc
     1321 ccggctcaac caggcgctgc gggagcgcac gcggctcgtc tcgagcatca acggctggta
     1381 cggagcgctg cagggggcgg gagccatcgg cgtcaccgcc cagctggaag ggcgggacgc
     1441 ggaggcggtg gagcgggcca tcctggcgga ggtcgagcgc gtccggtccg agggggtcac
     1501 cccggacgag ctggcgcggg ccatcaccgc gtcggaggcc gagcgcgagt tctcgcgcga
     1561 gacggtggag ggactcgccc tcgcgtacgg ccgcgccgag accacgtgga acctggagga
     1621 ggagcgagcc tacgtggacc gcgtgcgcaa ggtgacacgg gcggacgtgc aggcggcggc
     1681 gcgccgctac ctgaccccct cgtacgcgcg gctcaccttc gttccgcggg cccggacccc
     1741 gtgaggacgt cgcggctggt ctcgacggcg gtgctcgccg cgacggccgc gtgggctctg
     1801 gccggcgtgg cggcggccca ggggccccgg cgcgagcagc tgccgagcgg cctcaccgtg
     1861 ctggtgcgcg agaacaccgc caccccggtc gtcgccgcct cgctgttcgt gcgcgtcggc
     1921 tcccggtggg agacggaggc cgacgcgggc atcacccacc tgctgcagca ggtgctgctc
     1981 aagggaaccc tgacccgctc ggccctcgag atcgccgaga ccgccgaggg actgggcggg
     2041 ggaatcagcg catcggcgga catggacttc tccgagctgc gcgccaccgc gctcgcccgg
     2101 aactggaaga agatgatcga gctgatggcc gacgtcgcgc tccggccgac gctgccggac
     2161 gcggagatcg acggcgagcg gcgggcgatg ctgaccgcgc tgcgcagccg gcaggaccag
     2221 ccgttcccgc tcgccatgga caccgtcatg agccgcgtgt acggcgacca tccctacgga
     2281 cgcccggtcc tcgggcgacc ggccgcgctc gagcgcgtcg atcgcgccgc cctgctcgcg
     2341 taccaccagc gcttctaccg cgccccgcgg atgatcctgg cggtgagcgg ggacgtcagc
     2401 gcgcgggagg tgatggccga ggtcacgcgc cggttcgcga cggcgcccac cggagaaggc
     2461 gatcccgagc ccacgctgcc cgccgcggcg gcgcgcgccg accgtaccgt cctcgtgagg
     2521 ccatccgcgc aggcgcaggt actggtcgcg tttctcgccc cgcccacgtc gcaccccgac
     2581 tacgcggcgg tgaaggtgct ggccaccgcc ctgggcggcg gcatggccgg gcgcctcttc
     2641 accgaggtgc gcgacaagca gggcctggcc tactcgaccg gcggcgccta tccctcccgg
     2701 ctgggaccgg gggtcctcta tatgcagctc ggcaccgccc ccgcgaatca ggtccgcgcc
     2761 gaggcggcca tgctgggcga gctggagcga atccgacgcg atccggtgaa tcccgccgag
     2821 ctgacccgcg ccaaggcgta cctgctcggg cagttcgcgc tcgaccggcg aaccaacgcg
     2881 cgcctggcct ggtacgacgc cttcttcgag gtgctcggcg tcgggctcga cttctccgac
     2941 cgctacgccc gggcggtgga ggcggtcagc gcggagggcg tgctccgcgt ggcgcgtacc
     3001 tatctcgcgg cgccgacgat cgtgaccctc gggccggccg cccagtaggc cggcgtgttc
     3061 cctctcaagg acgacactcc
//
LOCUS       DATMCV010000861         2291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_246038, whole genome shotgun sequence.
ACCESSION   DATMCV010000861 DATMCV010000000
VERSION     DATMCV010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246038"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VNU03_19755"
     CDS             <1..676
                     /locus_tag="VNU03_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76767.1"
                     /translation="LQLREYLLPAPLRVVQAMTGDEIPWLRHLSITSVEIVGAFALAG
                     AVGVFLGVAVAWSPILSRALVPFLVFVNTLPKVAVAPLFLLWLGYGVLPNMLIGALIG
                     FFPVVINTAVGLSQVDEELLDLGRVFNAPKWKVFATIRIPNAYPYILSALKVTATAAV
                     VGAIVGEFVASQQGLGYVIITTQGSMNTPVAFAALVWISVVGLAVYGLVVLAARRIAP
                     WAETVE"
     gene            707..1726
                     /locus_tag="VNU03_19760"
     CDS             707..1726
                     /locus_tag="VNU03_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76768.1"
                     /translation="MRRVSLSIALLVAVVLVALALGSPSAQPAQQKVVFALNWFPVGD
                     HAAYWVALEKGYYRQRGLDVDMQNSKGSGDSIAKVDTGRADVGLADSAVVIASSARGT
                     KVKVVGMVFDKSPLNVWSRKDAPITKPKDLEGKTVAAPPGDGQRQMFPAFARLNGIDP
                     TKVTWVNVEPAAKVPALAEKRVDAVADYTTGLPFYEKAVGKGNAVMLPWADHGFDMYS
                     MSIIASEKTMKERAPMLRAFLEASYLGWRDVMSDPKSALEIFKKRVPEIDLSIIEPNM
                     MMGLELMKTDRYAKNGIGWMEEKKMCDSVDLVNTYMGVPTKVECKAVYTNEFLTKVEL
                     PKSAR"
     gene            1735..>2291
                     /locus_tag="VNU03_19765"
     CDS             1735..>2291
                     /locus_tag="VNU03_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP76769.1"
                     /translation="MGDLIRLDGVGMTYATASGPVEALRDIDLAVGRGELVAIVGPSG
                     CGKSTLLRIIAGLRAPSRGQVQVDGRPVVRPIPSVGMVFQAPVLLKWRTIEDNVLLPA
                     ELSRLDPRDYRARAAQLLRLVGLAEFAQKLPRELSGGMQQRASLCRALLLDPPLLLMD
                     EPFGALDAMTRDEMNLELLRVWGQG"
BASE COUNT          340 a          746 c          839 g          366 t
ORIGIN      
        1 gctccagctg cgcgagtatc tgctccccgc gccgctccgc gtggtccagg ccatgacggg
       61 cgacgagatc ccgtggctcc ggcatctctc gatcacgtcg gtggagatcg tcggggcgtt
      121 cgcgctggcc ggcgcggtgg gggtgttcct cggggtcgcg gtggcgtggt cgccgatcct
      181 gtcgcgggcg ctggtgccct tcctggtgtt cgtgaacacg ctgccgaagg tggcggtggc
      241 cccgctcttc ctcctctggc tcggctacgg cgtgctgccc aacatgctga tcggcgccct
      301 gatcggcttc tttccggtgg tgatcaacac cgcggtcggc ctctcccagg tggacgagga
      361 gctgctcgac ctcggccgcg tgttcaacgc gccgaagtgg aaggtcttcg cgacgatccg
      421 gatccccaac gcctacccgt acatcctgag cgcgctgaag gtgaccgcga ccgcggcggt
      481 ggtgggcgcc atcgtgggcg agttcgtggc ctcccagcag gggctcggct acgtgatcat
      541 caccacccag ggcagcatga acacgccggt ggccttcgcg gcgctggtgt ggatctcggt
      601 ggtcggcctc gcggtgtacg gcctcgtcgt cctcgccgcg cggcgcatcg ccccgtgggc
      661 ggagaccgtg gagtagcgga acccgtggac taaaggagaa gaatccatgc gacgcgtctc
      721 gctctcgatc gctctcctgg tggcggtggt gctggtggcg ctcgcgctcg gcagcccgtc
      781 ggcgcagccg gcgcagcaga aggtggtctt cgcgctcaac tggttcccgg tgggcgacca
      841 cgcggcgtac tgggtggcgc tcgagaaggg ctactaccgc cagcgaggcc tcgacgtgga
      901 catgcagaac tccaagggct ccggcgactc catcgccaag gtggacaccg ggcgcgcgga
      961 cgtgggcctg gccgacagcg cggtggtcat cgcctcctcg gcgcggggca ccaaggtgaa
     1021 ggtggtcggc atggtgttcg acaagagccc gctgaacgtg tggagccgca aggacgcgcc
     1081 gatcaccaag ccgaaggacc tcgagggcaa gacggtggcc gcgccccccg gcgacgggca
     1141 gcgccagatg ttccccgcct tcgcgcgcct gaacggcatc gacccgacca aggtgacgtg
     1201 ggtgaacgtg gagccggcgg ccaaggtgcc ggcgctagcc gagaagcgcg tcgacgcggt
     1261 ggccgactac accaccggct tgccgttcta cgagaaggcg gtgggcaagg gcaacgcggt
     1321 catgctgccg tgggccgacc acggcttcga catgtacagc atgtcgatca tcgcgagcga
     1381 gaagacgatg aaggagcgcg ccccgatgct gcgggccttc ctcgaggcct cgtacctggg
     1441 ctggcgcgac gtcatgagcg acccgaagtc ggcgctcgag atcttcaaga agcgggtgcc
     1501 cgagatcgac ctctcgatca tcgagccgaa catgatgatg gggctcgagc tgatgaagac
     1561 cgaccgctac gcgaagaacg ggatcggctg gatggaggag aagaagatgt gcgactcggt
     1621 cgacctcgtg aacacctaca tgggcgtgcc gacgaaggtc gagtgcaagg cggtgtacac
     1681 caacgagttc ctcaccaagg tggagctgcc caagtccgcc cgctgaccgc gcgcgtgggc
     1741 gacctcattc gcctcgacgg ggtcgggatg acgtacgcga ccgcgtccgg ccccgtcgag
     1801 gcgctgcgcg acatcgacct cgcggtcggc cggggcgagc tggtggccat cgtgggccca
     1861 tcggggtgcg gcaagtcgac gctcttgcgc atcatcgcgg ggctgcgggc gccgagtcgc
     1921 ggccaggtcc aggttgacgg gcgcccggtg gtgcggccga tcccgtcggt cggcatggtg
     1981 ttccaggcgc cggtgctgct gaagtggcgg acgatcgagg acaacgtgct gctgcccgcc
     2041 gagctctcgc ggctcgatcc ccgcgactac cgcgcccggg ccgcgcagct gctccgcctg
     2101 gtgggcctcg ccgagttcgc gcagaagctg ccgcgcgagc tgtcgggggg catgcagcag
     2161 cgggcctcgc tctgccgcgc gctgctgctg gatccgccgc tcctcctaat ggacgagcct
     2221 ttcggggcgc tcgatgcgat gacccgcgac gagatgaatc tcgagctgct gcgcgtgtgg
     2281 ggccagggga g
//
LOCUS       DATMCV010000862         2338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_45122, whole genome shotgun sequence.
ACCESSION   DATMCV010000862 DATMCV010000000
VERSION     DATMCV010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45122"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /gene="tilS"
                     /locus_tag="VNU03_19770"
     CDS             complement(<1..1118)
                     /gene="tilS"
                     /locus_tag="VNU03_19770"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWP76770.1"
                     /translation="MTIVTSLVARVQDTIRRHGMLRGGEAVLVAVSGGPDSVALLDVL
                     CALRAPLALSLSVAHVHHGLRPEADAEAEAVARLCEQLGVACRVERVTVRQAPPWDGL
                     EAESRRARHAALARAAHAAGAARIATGHTADDQAETVLMRLLQGAGPRGLGGIAPVRG
                     RLVSPLIETRRGEIVAHLAARGLAAAEDASNRDPRFLRSRVRHDLLPFMAELTGGSVV
                     EALGRSAVAARAVMADLEARARADLERMATRGPAGFILDVSPLEAQPLELAAETIRQA
                     AALLGETRPLRGPAQRALRRLLGETPRRRAVRLGRLVVERSGRRLRVGPPALSAVTTR
                     GWPGAGVLDMPEIGQRLTARVLARDADYVVPREPARAA"
     gene            complement(1102..1797)
                     /locus_tag="VNU03_19775"
     CDS             complement(1102..1797)
                     /locus_tag="VNU03_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWP76771.1"
                     /translation="MKVLVRLFARYREAAGRDSLDLELPDGGTVERAWEAVTRQLPAL
                     TPYRPFTLFALRNDYVDADRLLADGDELCLFPPVSGGSAPADADWIEVTTEPLSERAL
                     AQAVEHPGAGALALFSGVVRDQTGGRRVKFLEYEAHGPMALAKMREIASAIRGRWPII
                     NRIALVHRIGRLEIGESSVMIAISSPHRGEAFEACRFAIDTLKETVPIWKKEYFEDGE
                     VWVGLQSECDHRH"
     gene            complement(1824..2150)
                     /gene="erpA"
                     /locus_tag="VNU03_19780"
     CDS             complement(1824..2150)
                     /gene="erpA"
                     /locus_tag="VNU03_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HWP76772.1"
                     /translation="MITLTETAAKKISDLRLEEGKPEWGLRIRIVGGGCSGMSYELGW
                     DDTATDGDNVVETNGVKVFVDSNSAQYLQGSEIDYVDNNMLGAGFAIKNPNVKSSCGC
                     GSSHQF"
BASE COUNT          322 a          866 c          852 g          298 t
ORIGIN      
        1 aaggcggcgc gcgcgggctc ccgcgggacc acgtagtcgg catcccgcgc gagcacgcgc
       61 gcggtgagcc gctggccgat ctccggcatg tcgagcaccc ccgcccccgg ccacccgcgc
      121 gtggtgaccg cggagagcgc cggaggaccg acccgcagcc ggcggccgct ccgctcgacc
      181 acgagacggc cgagtcgcac cgcccggcga cgcggcgtct cgcccagcag ccggcggagc
      241 gcgcgctggg caggtccgcg gagggggcgc gtctcgccca gcagggccgc ggcctgccgg
      301 atcgtctccg cggccagctc gaggggctgg gcctcgagcg gcgacacgtc gaggatgaac
      361 ccggccggtc cccgggtggc catccgctcg agatcggcgc gcgcccgcgc ctcgaggtcg
      421 gccatcaccg cgcgcgccgc cacggccgag cgcccgagcg cctcgaccac cgagccgccg
      481 gtgagctccg ccatgaacgg cagcaggtcg tggcggacgc gcgagcgcag gaagcgcggg
      541 tcgcggttgc tcgcgtcctc ggccgcggcg aggccgcgcg cggcgagatg cgccacgatc
      601 tcgccccggc gggtctcgat cagcgggctg acgaggcgcc cgcggaccgg ggcgatgccg
      661 ccgaggccgc ggggcccggc gccctggagg agccgcatca gcaccgtctc ggcctggtcg
      721 tccgcggtgt ggccggtcgc gatgcgcgcg gcacccgcgg cgtgcgcggc gcgggccagc
      781 gcggcgtgcc gcgcccgccg gctctccgcc tcgagcccgt cccagggcgg agcctgccgg
      841 accgtgacgc gctcgacccg gcaggccacc ccgagctgct cgcagagccg cgcgaccgcc
      901 tccgcctccg cgtcggcctc cggccggagg ccgtggtgca cgtgggccac cgagagcgag
      961 agcgcgagcg gggcccggag cgcgcagagc acgtcgagga gcgcgaccga gtcgggcccg
     1021 cccgagacgg ccaccaagac ggcttcgccg ccacggagca ttccgtgacg gcgaatggtg
     1081 tcctggacgc gcgcgacgag gctagtgacg atggtcacac tcggactgca ggcccaccca
     1141 cacctcgccg tcctcgaagt actccttctt ccagatcggc accgtctcct tcagggtatc
     1201 gatggcgaag cggcaggcct cgaaggcctc gccgcggtgg ggcgaggaga tcgcgatcat
     1261 cacgctcgac tccccgatct cgaggcggcc gatgcgatgc acgagggcga tgcggttgat
     1321 gatgggccag cggccgcgga tggccgacgc gatctcccgc atcttggcga gtgccatcgg
     1381 cccgtgggcc tcgtactcga ggaacttgac ccgccgaccc ccggtctggt cgcgcaccac
     1441 tcccgagaag agcgcgaggg cgcccgcgcc cgggtgctcc accgcctggg cgagcgcacg
     1501 ctccgagagg ggctcggtgg tgacctcgat ccagtccgcg tccgccggcg cggagccgcc
     1561 gctcaccggt gggaagagac agagctcatc gccgtctgcg agcagccggt cggcgtcgac
     1621 gtagtcgttg cggagcgcga agagggtgaa cggccggtag ggggtgagcg cgggaagctg
     1681 gcgggtcacc gcttcccagg cccgctcgac ggtgccgccg tcgggaagct cgagatccag
     1741 cgagtcacgg cccgccgcct cgcgatagcg ggcgaagagc cggaccagca ccttcatcga
     1801 gcgagcctcg atcccgccgc ggcttagaac tggtgggacg agccgcagcc gcaggacgac
     1861 ttcacgttcg ggttcttgat cgcgaacccg gcgccgagca tgttgttgtc gacgtagtcg
     1921 atctcgctac cctgcagata ctgggcgctg ttcgagtcca cgaagacctt cacgccgttg
     1981 gtctcgacga cgttgtcgcc gtcggtcgcg gtgtcgtccc agccgagctc gtaggacatg
     2041 ccggagcatc cgcccccgac gatacggatc cgcagccccc actcgggctt gccctcttcg
     2101 agccgcaggt ccgagatctt cttggcggcc gtctcagtca acgtgatcat ccaatgcctc
     2161 cttgggtgga gtgccgaact gtcgagtcgc gctcattata gcagaaggac cgtgggcttg
     2221 ccgggcgccc tccctgccgg ccggcggcct agaggatgcc tcccgcgggc agcagcgtga
     2281 cctcctcgag gctcgcattc gggcccagtc ccgccatgag cagcgccgcc tcggccac
//
LOCUS       DATMCV010000863         5897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_383604, whole genome shotgun sequence.
ACCESSION   DATMCV010000863 DATMCV010000000
VERSION     DATMCV010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383604"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VNU03_19785"
     CDS             <1..426
                     /locus_tag="VNU03_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWP76773.1"
                     /translation="NDLAPVASPDYPYVVTTYRLTEHHLSGVMSRYLPMLAELFHSHF
                     AEISNELAAELGIKNGERITVATPRGKIHTTAMVTSRLKPFMIDGKKTHQIGIPWHWG
                     YNGISKGDITNDLSSTVGDPTVYIQETKAFLCSVKKGEV"
     gene            429..1208
                     /locus_tag="VNU03_19790"
     CDS             429..1208
                     /locus_tag="VNU03_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWP76774.1"
                     /translation="MARTLAMFTDTSLCIGCRACQVACKQWNELPMEEPEWTGSYQNH
                     AHFTDKTYRLVRFIEKPQANGELQWLLMSDVCKHCAQAGCLDACPTGAIHRTEYGTVN
                     INQDVCNGCRYCVSSCPFGVVSFNHDTGRANKCTFCNDRIHNGLGPACAKTCPTESIQ
                     FGFRDELAAKAEKRVAKLKEMGYTGAQLYGADTSGPLGGLNAFFLLLDRPSTYNLPEK
                     PLLPQRNVFVDSVLAIGAALVVGVGAMVSFRERGGKGDGDA"
     gene            1201..2019
                     /gene="nrfD"
                     /locus_tag="VNU03_19795"
     CDS             1201..2019
                     /gene="nrfD"
                     /locus_tag="VNU03_19795"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWP76775.1"
                     /translation="MLKSPEWHLLIVWYFFLGGIAGGAYFTAAIADNFGGPRDRNVVR
                     TGYILSLLMIGVCGMLLILDLGTPSRFMNMMGTFKFKDPMSIGAWMLGVFGLFSFVSS
                     ALSLVGGEERAGLRRKWSLVGILFGFFLASYTGVLLSATALPFWRDGRLMGALFLASG
                     ASTGMAAISLLLYLSGDSAGEGFKKVKRADRFAIVFEMVVLALFLVLLGSAAAPLTSG
                     HLAPLFWGGLVALGLVIPLLLDLVGGLRVPAVVPAVLVLVGGFVLRYVIVMSHA"
     gene            2111..2869
                     /gene="fdhD"
                     /locus_tag="VNU03_19800"
     CDS             2111..2869
                     /gene="fdhD"
                     /locus_tag="VNU03_19800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWP76776.1"
                     /translation="MRTYFRVKGGHLEEVKGEVVREQPLTVHVNGERFVTLLCSPFQL
                     EALVVGYLWMEKVIEDLSEITRIDISEVDGRAEVTLAGPVTLPTERILTSGCGGGITF
                     RIDPRLFPRLRSSVRVRPEQLAARLKELFGRAVHYQASRGIHGAALADAERLLVVAED
                     VGRHNAVDKVKGEALLRSIPTTDRVLISTGRISSEMLLKAARMGVPVVASRTSPTEMA
                     VGLAEQLGITVIGYVRPDGLNLYAGDALDADGGP"
     gene            2873..3757
                     /locus_tag="VNU03_19805"
     CDS             2873..3757
                     /locus_tag="VNU03_19805"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HWP76777.1"
                     /translation="MPDYSALMADWQALLARRPALGEPLRFWTAILEGWAGWKPPATL
                     VPLAWSADECRGRWERGVPLLAEARPIIPSASVEDLLGPVMERLAADGPEAVEAFRRL
                     VLAWDEGQIDASALLPAPDRDPAAWLLDRFGLGTHLGAFLPVTALRPALEIYFEGVRA
                     LPDGIWTRGACPWCGGAAGYGDLLEDGRRRLSCHLCGGAWIAARLRCPFCESWNSRDL
                     ARLVAEDLEAAYFIEGCRACRGYVKGVDRRQRWNAGPPLVEDWGSPHLDLHASREGYW
                     RATPSLAHLLPPETGETA"
     gene            3754..4560
                     /gene="ccsA"
                     /locus_tag="VNU03_19810"
     CDS             3754..4560
                     /gene="ccsA"
                     /locus_tag="VNU03_19810"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWP76778.1"
                     /translation="MNAVLFDVALTAYILAAVAAIGSLAGRRDQLAWVALLLTQAGWI
                     CHTGAVILRGIELRRLPVSTLAEMVSLLIWAVILLDFWVERQRRIRPLSAFVLPVVLA
                     LGLGLPTGLRSIVLEPPVRSGWIVVHVALVLVGLAALVLNFGGALMYLLVERQLKAKR
                     PGPAYYRLPPLETLDRLTIATLTVAFPFLTVGLALGVLSARGAWGSVVAFDPLALFSL
                     VMWIIYAATLFGRVLGHWRGRRAAYLSIAGFCVMLVTLSAGAFLHGHHGS"
     gene            4550..5869
                     /gene="hemA"
                     /locus_tag="VNU03_19815"
     CDS             4550..5869
                     /gene="hemA"
                     /locus_tag="VNU03_19815"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWP76779.1"
                     /translation="MAPDERVPDAPGLLFVAGMNHRTAPVAVREQLALEEEKIREILA
                     DLTGRGFLHEVMILSTCNRVEVYGVAAVPGEARSQAFSRLGSHRGMAWRELEPLLYTA
                     TGDEAALHAFRVAASLDSMVLGEPQILGQVKDAFALAQSAGTAGPVLHALMSQAFSAA
                     KRVRSETLVGRLAVSISYAAVELARRIFEGLEGKSVLLVGAGEMSELAARHLIEQGAL
                     PVYVANRTWSRAQELARGLGGVPVPFDQIEATLARVDIVITSTAAPEPIVTAAQVRAA
                     LQARRGQPLFFIDIAVPRNVEPAVNDLEGAFCYDIDDLRAVVESNLKERQREAQRAQT
                     LLEREVDKFAGRLQQLEVVPTIVSLREKLEAIRRAELERALARLPGVAEDTRRVMDAL
                     SQAIVNKVLHAPMVKLKDSSRAGHGRRWTETISELFGLRGPGPGPSE"
BASE COUNT          761 a         2127 c         2070 g          939 t
ORIGIN      
        1 aacgacctgg ccccggtggc ctcgccggac tatccctacg tggtcaccac ctaccgcctc
       61 accgagcatc acctctcggg ggtcatgtcg cgctacctgc ccatgctcgc cgagctgttc
      121 cactcgcact tcgcggagat cagcaacgag ctggccgccg agctcgggat caagaacggg
      181 gagcggatca cggtcgcgac cccgcgcggg aagatccaca ccaccgcgat ggtcacgagc
      241 cggctcaagc cgttcatgat cgacggcaag aagacccacc agatcggcat cccctggcac
      301 tggggctaca acggcatctc caagggcgac atcaccaacg atctctcctc gacggtggga
      361 gaccccacgg tctacatcca ggagaccaag gccttcctct gcagcgtgaa gaagggggag
      421 gtgtagccat ggcccgcacc ctcgcgatgt tcaccgacac gtccctctgc atcggctgcc
      481 gcgcatgcca ggtggcgtgc aagcagtgga acgagctgcc gatggaggag ccggaatgga
      541 ccggctccta ccagaaccac gcccacttca ccgacaagac ctatcgcctg gtgcgcttca
      601 tcgagaagcc gcaggcgaac ggcgagctgc agtggctcct gatgtcggac gtttgcaagc
      661 actgcgccca ggcgggctgc ctcgacgcct gcccgaccgg cgcgatccac cgcaccgagt
      721 acggcaccgt gaacatcaac caggacgtgt gcaacggctg ccggtactgc gtcagctcgt
      781 gcccgttcgg ggtcgtctcg ttcaaccacg acaccggccg cgccaacaag tgcaccttct
      841 gcaacgaccg catccacaac ggcctcgggc ccgcgtgcgc caagacctgt cccaccgagt
      901 ccatccagtt cggcttccgg gacgagctgg ccgcgaaggc cgagaagcgc gtggccaagc
      961 tcaaggagat gggctacacg ggcgcccagc tctacggcgc ggacaccagc ggcccgctgg
     1021 gcgggctgaa cgcgttcttc ctcctgctgg accggccgtc cacctacaac ctgcccgaga
     1081 agccgctgct gccgcagcgc aacgtcttcg tcgactcggt gctcgcgatc ggggccgccc
     1141 tcgtggtggg ggtgggcgcg atggtctcgt tccgcgagcg gggcgggaag ggggacggcg
     1201 atgcttaaga gcccggagtg gcacctgctg atcgtctggt acttcttcct gggcggtatc
     1261 gcgggcggcg cctacttcac cgccgccatc gcggacaact tcggcggccc gcgcgaccgc
     1321 aacgtggtgc gcaccggcta tatcctctcg ctcctgatga tcggggtgtg cggcatgctc
     1381 ctgatcctcg atctgggcac gcccagccgg ttcatgaaca tgatggggac cttcaagttc
     1441 aaggatccca tgtcgatcgg ggcctggatg ctcggggtct tcggcctctt ctcgttcgtt
     1501 tcctccgcgc tgtccctcgt gggcggtgaa gagcgggccg gcctccggcg caagtggagc
     1561 ctggtcggga tcctcttcgg gttcttcctc gccagctaca ccggggtgct actgagcgcc
     1621 accgcgctgc cgttctggcg cgacgggcgc ctgatgggcg cgctcttcct cgcctcgggc
     1681 gcctccaccg gcatggccgc catctcgctg ctgctctacc tgagcggaga ttcggcgggg
     1741 gagggcttca agaaggtcaa gcgggcggac cgcttcgcca tcgtcttcga gatggtggtc
     1801 ctggctctct tcctggtcct gctcggctcg gcggctgccc cgctcacgtc ggggcacctg
     1861 gctccgctct tctggggcgg cctggtcgcg ctcgggctgg tcatcccgct cctgctcgac
     1921 ctcgtcggcg gcctgcgggt gcccgcggtg gttcccgcgg tgctggtgct cgtgggcggc
     1981 ttcgtcctgc gctacgtcat cgtcatgagc cacgcctgag cccgccgtcc ggcgggccgg
     2041 tcgcccggtc gcacaagggg ccccgagagg ggccccttcc tttatactga ggcccggcag
     2101 ccagcggacc atgcgcacct actttcgcgt caagggcggc cacctcgagg aggtcaaggg
     2161 cgaggtcgtc cgggagcagc cgttgacggt ccacgtcaac ggggagcgct tcgtcacgct
     2221 gctctgctcg ccgttccagc tggaggcgct ggtggtgggc tacctctgga tggagaaggt
     2281 gatcgaggat ctctccgaga tcacccggat cgacatctcc gaggtggacg ggcgcgccga
     2341 ggtgaccctg gccgggccgg tgaccttgcc caccgagcgc atcctgacct cgggctgcgg
     2401 cggcggcatc accttccgga tcgacccccg cctgttcccg cgcctgcgct cttcggtccg
     2461 ggtccgcccc gagcagctcg cggcgcggct caaggagctg ttcgggcgcg cggtccacta
     2521 ccaggcctcc cgcggcatcc acggggcggc cctggccgac gcggagcggc tgctggttgt
     2581 cgcggaggac gtcggccgtc acaacgcggt ggacaaggtc aagggcgagg cgctgctccg
     2641 ctcgatcccg accacggatc gcgttctcat ctccaccggg cggatctcgt ccgagatgct
     2701 gctcaaggcc gcgcgcatgg gcgtgccggt ggtcgcctcg cgcacctcgc ccaccgagat
     2761 ggcggtgggg ctggccgagc aactcggcat caccgtgatc gggtacgtgc ggcccgacgg
     2821 actcaacctc tacgccggcg acgcgctcga cgccgacggc ggcccctgac ggatgccgga
     2881 ctactccgcg ctgatggccg actggcaggc gctgctcgcg cggcgccccg cgctcggcga
     2941 gcccctccgc ttctggaccg cgatcctcga gggctgggcc ggatggaagc cgcccgccac
     3001 cctcgtgccg ctcgcctggt ccgccgacga gtgccggggg cggtgggagc gcggggtgcc
     3061 gctcctggcc gaggcccggc cgatcatccc ctccgcctcg gtcgaggacc tgctggggcc
     3121 ggtcatggag cgattggccg ccgacgggcc ggaggcggtg gaggccttcc gccgcctcgt
     3181 gctcgcgtgg gacgaagggc agatcgacgc gagcgcgctg ctgcccgcgc ccgatcgcga
     3241 tccggccgcg tggctgctcg atcgattcgg actcggcacg cacctgggcg cgttcctccc
     3301 ggtgaccgcg ctgcggcccg ccctcgagat ctacttcgag ggcgtccgcg ccctgccgga
     3361 cgggatctgg acgcgcggag cgtgcccgtg gtgcggcggg gccgccggct acggcgacct
     3421 gctggaggat ggacgccggc ggctctcctg ccatctctgc ggcggggcgt ggatcgcggc
     3481 gcggctgcgc tgcccgttct gcgagagctg gaactcgcgc gacctggcgc gcctggtcgc
     3541 ggaggatctc gaggcggcct acttcatcga gggctgtcgc gcgtgccgcg gctatgtgaa
     3601 gggcgtcgat cggcgccagc gctggaacgc ggggcccccg ctcgtggagg actggggctc
     3661 cccgcacctc gatctccacg cctcgcgcga gggctactgg cgcgccaccc cctcgctcgc
     3721 gcacctgctc ccgcccgaga ccggagagac ggcgtgaacg cggtgctctt cgacgtggcc
     3781 ctgaccgcct acatcctggc cgcggtggcc gcgatcggct cgctggccgg gcggcgcgac
     3841 cagctcgcgt gggtcgcgct gctgctcacc caggcgggct ggatatgcca caccggcgcg
     3901 gtgatcctgc ggggcatcga gctccggcgc ctgccggtct cgaccctcgc cgagatggtc
     3961 tcgctgctga tctgggccgt gatcctcctc gatttctggg tcgagcgtca gcggcgcatc
     4021 cgcccgctca gcgccttcgt gctcccggtc gtcctggccc tgggcctcgg gctgcccacc
     4081 gggctccgct cgatcgtgct cgagcccccg gtgcgcagcg gctggatcgt ggtgcacgtg
     4141 gcgctggtgc tggtggggct ggccgcgctc gtgctgaact tcgggggcgc gctcatgtac
     4201 ctgctggtgg agcgccagct caaggccaag cgccccggcc ccgcgtacta tcgcctgccg
     4261 ccgctcgaga cgctcgaccg gctcacgatc gccaccctca ccgtcgcctt cccgttcctc
     4321 acggtgggcc tcgccctcgg cgtgctctcg gcccgcgggg cgtggggcag cgtggtcgcg
     4381 ttcgacccgc tcgccctctt ctcgctggtg atgtggatca tctacgccgc gacgctgttc
     4441 ggccgcgtgc tcgggcactg gcgcggccgg cgggccgcct atctctccat cgccggcttc
     4501 tgcgtgatgc tggtcacgct gagcgcgggc gccttcctcc acgggcacca tggctcctga
     4561 cgagcgggtc cccgacgcgc ccggtctcct gttcgtcgcg gggatgaatc accgcaccgc
     4621 gccggtggcg gtgcgcgagc agctggccct cgaggaggag aagatccggg agatcctcgc
     4681 cgatctgacc ggccgcggct tcctgcacga ggtcatgatc ctctccacct gcaaccgggt
     4741 cgaggtctac ggggtggccg cggtcccggg cgaggcgcgc tcccaggcgt tcagccggct
     4801 cgggagccac cgcggcatgg cgtggcggga gctggagccg ctgctctaca ccgcgaccgg
     4861 cgacgaggcc gcgctccacg ccttccgcgt cgcggcgagc ctcgactcca tggtgctggg
     4921 cgagccgcag atcctcgggc aggtgaagga cgccttcgcg ctggcccagt cggcgggcac
     4981 cgcgggcccg gtgctgcacg ccctcatgag ccaggcgttc agcgcggcta agcgggtgcg
     5041 atccgagacc ctggtgggcc gcctcgcggt ctcgatctcc tacgcggcgg tggagctggc
     5101 gcggcggatc ttcgaggggc tcgagggcaa gtcggtcctg ctggtcggcg cgggcgagat
     5161 gagcgagctg gccgcgcggc acctgatcga gcagggcgcg ctgccggtct acgtggccaa
     5221 ccgcacgtgg agccgcgccc aggagctggc ccgcgggctc ggcggggtgc cggttccctt
     5281 cgatcagatc gaggcgacgc tcgcccgggt ggacatcgtg atcacctcca ccgcggcccc
     5341 cgagcccatc gtgaccgcgg cgcaggtgcg ggccgcgctc caggcgcgcc gcgggcagcc
     5401 gctcttcttc atcgacatcg cggtgccccg caacgtggag ccggcggtga acgacctgga
     5461 gggcgccttc tgctacgaca tcgacgacct gcgcgcggtc gtggagtcca acctcaagga
     5521 gcggcagcgg gaggcccagc gcgcccagac cctgctcgag cgcgaggtcg acaagttcgc
     5581 gggacggctg cagcagctcg aggtcgttcc caccatcgtg tcgctgcgcg agaagctgga
     5641 agcgatccgg cgcgccgagc tggagcgcgc actcgcccgc ctgcccggtg tggccgagga
     5701 cacgcgccgg gtcatggacg cgctcagcca ggccatcgtg aacaaggtgc tccacgcccc
     5761 catggtcaag ctcaaggact cgtcgcgcgc gggccacggc cgccgctgga cggagacgat
     5821 ctcggagctg ttcgggctgc gcggacccgg gcccggcccg tccgaatgaa cgtccgcctc
     5881 ggcacgcggg gcagccg
//
LOCUS       DATMCV010000864         2142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_313661, whole genome shotgun sequence.
ACCESSION   DATMCV010000864 DATMCV010000000
VERSION     DATMCV010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313661"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..334)
                     /locus_tag="VNU03_19820"
     tRNA            complement(259..334)
                     /locus_tag="VNU03_19820"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Lys,seq:ctt)
     gene            complement(376..452)
                     /locus_tag="VNU03_19825"
     tRNA            complement(376..452)
                     /locus_tag="VNU03_19825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(416..418),aa:Arg,seq:ccg)
     gene            complement(480..556)
                     /locus_tag="VNU03_19830"
     tRNA            complement(480..556)
                     /locus_tag="VNU03_19830"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(520..522),aa:His,seq:gtg)
     gene            complement(619..1506)
                     /locus_tag="VNU03_19835"
     CDS             complement(619..1506)
                     /locus_tag="VNU03_19835"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP76780.1"
                     /translation="MRALVIGGSGQVGAALMRVLRARGHDAAGTWAHHAYPGLVPLDL
                     ADVAALERLLAEQRPDWVLCPAALSHVDYCEDHPDEAFAINLQAPLAAARAAARAGAG
                     FVYYSSDYIFDGVGGPYTEEAPPRPLGVYGRSKAEGEQAVLGALDRALVVRTSVVYGP
                     ERQEKNFVYQLIRACRGGQGFRPAVDQRASPSYNVDVATATVECCEREERGIWHLAGA
                     DTLDRMAFSRLVCRVFDLDGSRLTPATTAELGQKAGRPLDGGLDVGKARRLLKTPLRG
                     AEEGLRAMRTALDAGPVTG"
     gene            1547..>2142
                     /locus_tag="VNU03_19840"
     CDS             1547..>2142
                     /locus_tag="VNU03_19840"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76781.1"
                     /translation="MAADRPLVLLWIPEAGPYRQAIERAGLDRRVELVSVPPTETPAP
                     ELLARAQAMLAYRSGPGVLPRMPALRWIQALTAGVEGWLALPDLPERLILTCARGTHR
                     VQMPENILGALFHLTKPYAGIALDQKEHRWRRRVSETLAGTTLGILGLGVIAQELARK
                     AAALEMRVIGTRRSPAPIAHVDCVYGLEGTDEVLATSD"
BASE COUNT          324 a          757 c          751 g          310 t
ORIGIN      
        1 agcgtggagc ctagcggccg gcctgaaacc acatccggac gaactggcta tccgggtatg
       61 ggatgacgag cccggctggg aggctgagtc cgacgagtat ccctcaagcg ggactatcag
      121 gttgtatccg cgggagtagc cggctgccgt ttgaggccgg gcggggcgcg cgagcaacga
      181 gtatctcggg accgtcagcg tggcgtcagc ggggttagcg agcaccttct gcgatgcccg
      241 ctaaccccgt gactgcattg gtgggccgtc aaggactcga accttggacc cgctgattaa
      301 gagtcagctg ctctaccaac tgagctaacg gcccgcgacg tagcgggtac tgccaccagt
      361 atctacagca gaaaatggtg cgcccaagag gattcgaacc tctgaccttt agctccggag
      421 gctaacgctc tatccagctg agctatgggc gcccgagcct gccacctggc tttcaggagt
      481 ggggtgaccg aagggaatcg aaccctcaac ccctggagcc acagtccagt gctctaacca
      541 attgagctac ggtcaccgtc ccgggacgac agccaagttg tgactctacc gtatgtttcc
      601 gggcgggtca acggccgctc agcccgtcac cgggcccgcg tccagcgccg tccgcatcgc
      661 gcgcagccct tcttccgcgc cccgcagcgg tgtcttcagg agacggcgcg ccttgccgac
      721 gtcgagccca ccgtcgagcg ggcggcccgc cttctgcccg agctctgccg tggtcgccgg
      781 agtgaggcgc gagccgtcga ggtcgaagac ccggcagacc aggcgcgaga aggccattcg
      841 gtcgagcgtg tcggcgccgg ccaggtgcca gatgccacgc tcctcccgct cgcagcactc
      901 gacggtggcg gtcgccacgt ccacgttgta ggaggggctc gcgcgctggt ccaccgcggg
      961 ccggaagccc tggccgccgc ggcaggcgcg gatgagctgg tagacgaagt tcttctcctg
     1021 gcgctcgggg ccgtagacca cgctggtgcg gaccaccagg gcccggtcga gcgcgcccag
     1081 caccgcctgc tcgccctccg ccttgctgcg cccgtagacg ccgagcggcc gcgggggcgc
     1141 ttcctcggtg tagggaccgc cgacgccgtc gaagatgtag tccgacgagt agtagacgaa
     1201 ccccgcgccg gcgcgcgccg ccgcccgggc cgcggcgagc ggggcctgca ggttgatcgc
     1261 gaacgcctcg tcggggtggt cctcgcagta gtccacgtgg ctgagcgcgg cggggcagag
     1321 cacccagtcg ggacgctgct cggcgagcag gcgctcgagc gcggccacgt cggcgaggtc
     1381 gagcggtacg agccccggat aggcgtgatg cgcccaggtg ccggccgcgt cgtggccgcg
     1441 cgcgcgcagc acgcgcatga gagccgcacc cacctgtccc gagccgccga tcaccagcgc
     1501 gcgcatgaat tctaggctac catcccgcgg ggaggaaccc acatcgatgg ctgccgatcg
     1561 tccgctggtg ctgctctgga tccccgaggc cggcccctat cgccaggcca tcgagcgcgc
     1621 cgggctcgac cggcgcgtgg agctggtctc cgtgccgccg accgagacgc ccgcgcccga
     1681 gctgctcgcg cgcgcgcagg ccatgctcgc gtaccgctcg gggccgggcg ttctcccgcg
     1741 catgccggcg ctccgctgga tccaggcgct gaccgccggc gtggagggct ggctcgccct
     1801 gcccgatctg ccggagcgcc tcatcctgac ctgcgcccgc gggacccatc gcgtgcagat
     1861 gcccgagaac atcctgggcg cgctcttcca cctgaccaag ccctatgccg gcatcgcgct
     1921 cgaccagaag gagcaccgct ggcggcggcg cgtatccgag acgctggccg gcaccacgct
     1981 cggcatcctg ggcctgggag tgatcgcgca ggagctggcg cggaaggccg cggcgctcga
     2041 gatgcgcgtg atcggcacgc gacggagccc ggcgcccatc gcacacgtgg attgcgtcta
     2101 cgggctggag ggcaccgacg aggtgctggc cacgtccgac tg
//
LOCUS       DATMCV010000865         6240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_180055, whole genome shotgun sequence.
ACCESSION   DATMCV010000865 DATMCV010000000
VERSION     DATMCV010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180055"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VNU03_19845"
     CDS             complement(<1..803)
                     /locus_tag="VNU03_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HWP76782.1"
                     /translation="MTAAGRGSVQTVLGPIAPADLGPTLMHEHLLCDIRHPSQRKPDD
                     LGPELALDNVWAINYGTAKGAARNYLLDARDVAIDEVRRMVDAGGRSVVDLSSGGLSP
                     DPMGLVQIARDSGAHVIMGCGQYVHDFQDPANATRTVEDFAEEMVEAVRDGVWGTDVK
                     AGIIGEIGCQAPWTAQEQRVMLGAVLAQRETGASINVHPGRHPDQPQEAADFIRVKGG
                     RMDRVIISHIDRTVFDDERLRRLADTGCVLEWDLFGQESSYYRHSDIDMP"
     gene            1315..2364
                     /locus_tag="VNU03_19850"
     CDS             1315..2364
                     /locus_tag="VNU03_19850"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP76783.1"
                     /translation="MIGRSTRPRSALLLVWIVLGAFVAALAPALPAHAQQTTLVVTGY
                     GGRWSEVMKKALVEPFEKKHNVKVEVVTGISTEWVAKLMASGPDNPPFDVLFGNEPAF
                     PIPRERGFFDKRNETLAPNIKNLYPKALIGDTSLAMFWGRIGLAYRTDSGVKKPTSWK
                     DFWDEAYVGKRSTYVIGNTLGINFLFVISKIFGKDYFDIDAGVAAIKKAQPKLVDFSG
                     TMEKQLEQKEAVLAVMHDAGTNDLKNRGIPVDWVAPVEGTPILDQVVQVTKGSKNREL
                     AWKFIDAYLSPEVQLAFASELFWSPTNKTVKLPADVAKKVIGPTDVDKLVLFDWAQVA
                     KQRPQWTEKWNREMR"
     gene            2361..3431
                     /locus_tag="VNU03_19855"
     CDS             2361..3431
                     /locus_tag="VNU03_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76784.1"
                     /translation="MTEGPRAAGTVTLTGLVKRYGTTLALDGVSLAVVPGEFFTLLGP
                     SGCGKTTTLRSVAGFVTPDAGRVAINGADVTGVPPHLRRVGMVFQHYALFPHRTVAQN
                     VGFGLRMQRVDRSEIARRVAEALALVQLPGHGGRYPSQLSGGEQQRVALARALVTRPS
                     VLLLDEPLGALDKKLRDHMKIELKRLQREVGITTIYVTHDQEEALTMSDRIAVMHRGR
                     VEQVAPPRGLYETPATAFVAGFIGNINLLSGRAAGANTVACGGATLAATGTAPAGTTV
                     AVALRPERIRLDPAAALDTVLPMTVAHVVYQGETVRYILKSEAGLELQAVELGEVRFA
                     TGARVRAGWAAADARMIQEETA"
     gene            3428..4105
                     /locus_tag="VNU03_19860"
     CDS             3428..4105
                     /locus_tag="VNU03_19860"
                     /inference="COORDINATES: protein motif:HMM:NF007788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HWP76785.1"
                     /translation="MTTVGLITVGQAPRSDVVPDMATILGRDVEIVEAGALDGLTRAE
                     MEPLAPEGDDEILVTRLADGTSVFVGKSKMITRIEAKIAALEDRGVALNVLLCTGEFP
                     KLRARRPFLEPQQLLLGLLRAMVFPGRLGVLTPSERHVPQTTARWRASGFDAHVAPLS
                     PYEETDPAAVRRAADALRAGNAGLVVMDCIGFRRKTRDEIAALTGAPTLVANLLVARV
                     AAELLGR"
     gene            4110..4955
                     /locus_tag="VNU03_19865"
     CDS             4110..4955
                     /locus_tag="VNU03_19865"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76786.1"
                     /translation="MTRGARLLLLGPYVVLLMVFFAAPLVLMFAISLSRQSFGQLEWA
                     FTLHHYVRFLTDAYYLGVLADTLWLGVLTTVVALLLGYPLAYHLALTRSRWKPLLIVF
                     ILSPLLVGIVIRCYGWMILLADRGLINETLAQHGWITKPLPLMYNKFGVTIALVHVFL
                     PFMVLSLTGVLKRIEPQLIEASQTLGASPRRAFFEVTLPLSLPGILAGSLLVFSLAIS
                     SFVVPILVGGFKVHVLPMVVYEQVLSVFDWPFGAANAFVLLVISVALIAVYIKVTERA
                     LRGIV"
     gene            4970..5725
                     /locus_tag="VNU03_19870"
     CDS             4970..5725
                     /locus_tag="VNU03_19870"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76787.1"
                     /translation="MGLVYAFLLLPMVVVVLAAFNAGNYFTFPPQGFSLKWFANFFQR
                     REFMQALRLSTELAVWTALASTVIGTAAAIPLVRGRFRGRDLLNAFVTSPLLLPQILT
                     GVALLQFYTLLKMQASYGALLLGHIVVTTPYVIRTVTATLTHFDMALEEAAQSLGAHP
                     VRAFFEVTLGVIKPGVVAGAIFAFAISFDNFTLSLFLTSSKLTPLPIELFAYLKYSFD
                     PTAAAVSAFAIGVALVLVLGIARFMGLEEFTGF"
     gene            5753..>6240
                     /locus_tag="VNU03_19875"
     CDS             5753..>6240
                     /locus_tag="VNU03_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HWP76788.1"
                     /translation="MRPVAAEEIESLAIGAWILGTGGGGSPYLALLNMRQLYRQGIVS
                     QLMDASELADDDLVAVVSNMGAPLVGQERLTDPKTMALAVTMMEEYLGRKFRALMSLE
                     IGGGNSIQPFMGAAMLDLPVIDGDCMGRAFPEAQMTSFAIHDLQMYPLTLADVRDNAV
                     VVA"
BASE COUNT          877 a         2272 c         2088 g         1003 t
ORIGIN      
        1 ggcatatcga tgtcggagtg acggtagtag gacgactcct ggccgaagag atcccattcg
       61 agcacgcagc cggtatcggc cagccgccgc agccgctcgt cgtcgaagac ggtccggtcg
      121 atgtggctga tgatcacgcg atccatccgc ccgcccttca cgcggatgaa gtcggccgcc
      181 tcctgcggct ggtcgggatg acgccccggg tgcacgttga tggacgcgcc ggtctcccgc
      241 tgggccagca ccgcgcccag catcacccgc tgctcctgag ccgtccacgg cgcctggcag
      301 ccgatctcgc cgatgatgcc ggccttcacg tcggtgcccc agacgccgtc gcgcaccgcc
      361 tcgaccatct cctccgcgaa atcctcgacg gtgcgggtgg cgttggcagg gtcctggaag
      421 tcgtggacgt actggccgca gcccatgatc acgtgcgccc cgctgtcgcg cgcgatctgc
      481 accaggccca tgggatcggg gctgagtccg ccggaggaga gatcgaccac gctgcgcccg
      541 ccggcgtcca ccatgcggcg cacctcgtcg atggccacgt cgcgcgcgtc cagcaggtag
      601 ttccgcgccg cgcccttcgc ggtgccgtag ttgatggccc acacgttgtc gagggccagc
      661 tcggggccga ggtcgtcggg cttgcgctgc gaggggtggc ggatgtcgca cagcaggtgc
      721 tcgtgcatca gcgtgggccc gaggtccgcg ggcgcgatgg ggccgagcac ggtctgcacg
      781 gagccccggc ccgccgcggt catggcgcac accgtacgcc gcggccggcg gggcgtcaat
      841 ggcgctccgt cgaccgcgaa ccgcgaccgg cgcggcgacg atccggtaag ccttgccgtg
      901 tagaacctcc ctcataacgc ggccggagcg ggccaacggg caccaacctg tgtccaatgc
      961 gcaatcaggc tcgggatggc atgtccgatg ctccatcacc tcgcatgagc cgcacgcgat
     1021 ccaagccacc gtgggcgcag gcgtcaccga gtagcgcgcc gggcacgcgg gcgaccaagc
     1081 ggtccgcaag ccgatcgggc cgggccgtgc gccgctttcc gcacctggtg gaggacatgc
     1141 cgccgcccgc ggtgccgccc gacgggcgac ggcggcggcc ggtgacccgc gggcggatcg
     1201 cgccgcgcta gccgcgctcg cgcccgcgcg cggcggccgc cctgcctcta ccattcgtcc
     1261 tccggctgcg acataatgcc gcccacgtca acgccacgtc ccggaggcct gctcatgatc
     1321 ggtcggtcga ctcgtccccg ctccgcgctg ctcctcgtct ggatcgtgct cggggccttc
     1381 gtggccgcgc tcgcgcccgc ccttcccgcc cacgcccagc agaccacgct cgtggtcacc
     1441 ggctacggcg gccgctggtc cgaggtcatg aaaaaggcgc tcgtcgaacc cttcgagaag
     1501 aagcacaacg tgaaggtcga ggtcgtcacc ggcatcagca ccgagtgggt ggccaagctc
     1561 atggcttccg ggcccgacaa cccgcccttc gacgtgctct tcggcaacga gccggccttc
     1621 ccgatcccgc gcgagcgcgg cttcttcgac aagcgcaacg agacgctggc ccccaacatc
     1681 aagaacctct acccgaaggc cctcatcggc gacacgagcc tcgccatgtt ctgggggcgc
     1741 atcgggctgg cctaccgcac cgactccggg gtcaagaagc ccacctcctg gaaggacttc
     1801 tgggacgagg cctatgtggg caagcgctcc acctacgtga tcggcaacac gctcggcatc
     1861 aacttcctgt tcgtgatctc gaagatcttc ggcaaggact acttcgacat cgacgcgggg
     1921 gtggccgcga tcaagaaggc gcagcccaag ctggtggact tctccggcac catggagaag
     1981 cagctcgagc agaaggaggc ggtgctcgcg gtgatgcacg acgcggggac caacgatctg
     2041 aagaaccgtg ggatcccggt ggactgggtg gcccccgtcg agggcacgcc catcctcgac
     2101 caggtcgtcc aggtcacgaa gggctcgaag aaccgggagc tggcgtggaa gttcatcgac
     2161 gcctatctct cgcccgaggt ccagctcgcg ttcgcgagcg agctcttctg gagcccgacc
     2221 aacaagacgg tgaagctgcc ggccgacgtg gccaagaagg tcatcgggcc caccgacgtg
     2281 gacaagctcg tgctcttcga ctgggcgcag gtggccaagc agcggccgca atggaccgag
     2341 aagtggaacc gggagatgcg ttgacggaag ggccgcgcgc cgccggcacc gtcaccctga
     2401 ccggcctcgt caagcgctac ggcacgactc tggccctcga cggcgtctcc ctggccgtcg
     2461 tgccggggga gttcttcacc ctgctcggcc cgtccgggtg cggcaagacc accaccctgc
     2521 gctcggtggc cggcttcgtg accccggacg ccggccgggt ggcgatcaac ggggccgacg
     2581 tcaccggcgt gccgccgcac ctgcgccgcg tcggcatggt gttccagcac tacgcgctct
     2641 tcccgcaccg caccgtggcg cagaacgtgg gcttcggcct gcgcatgcag cgggtggacc
     2701 ggtcggagat cgcgcggcgg gtggccgagg cgctggccct cgtccagctc cccggtcacg
     2761 gcgggcgcta cccgagccag ctctcgggcg gcgagcagca gcgggtcgcg ctggcgcgcg
     2821 cgctcgtcac ccggccctcg gtgctgctgc tggacgagcc gctgggcgcg ctcgacaaga
     2881 agctgcgcga ccacatgaag atcgagctca agcggctcca gcgcgaggtg ggcatcacca
     2941 cgatctacgt gacccacgac caagaggagg cgctcaccat gtccgaccgc atcgcggtca
     3001 tgcaccgcgg gcgcgtggag caggtggccc cgccgcgcgg cctctacgag acgcccgcga
     3061 ccgccttcgt ggccggcttc atcggcaaca tcaacttgct gtccggccgc gccgcgggcg
     3121 cgaacaccgt cgcgtgcggc ggagccaccc ttgcggccac cggcaccgcg ccggccggca
     3181 ccactgtcgc ggtcgccctg cgcccggagc ggatccgcct ggatcctgcc gccgcgctcg
     3241 acacggtgct cccgatgacg gtcgcccacg tggtctacca gggcgagacc gtccgctaca
     3301 ttctcaagtc cgaggccggg ctcgagctgc aggccgtcga gctcggcgag gtgcgcttcg
     3361 cgaccggcgc ccgcgtgcgc gcgggctggg ccgccgcgga cgcccggatg atccaggagg
     3421 agaccgcatg accacggtcg gactgatcac ggtcggacaa gccccgcgca gcgacgtggt
     3481 gccggacatg gccaccatcc tcggccgcga cgtcgagatc gtcgaggcgg gcgcgctcga
     3541 cgggctcacc cgcgcggaga tggagcccct cgcccccgag ggcgacgacg agatcctggt
     3601 gacccggctg gccgacggca cctcggtctt cgtcggcaag agcaagatga tcacgcgcat
     3661 cgaggcgaag atcgccgcgc tcgaggatcg gggcgtcgcg ctgaacgtgc tgctgtgcac
     3721 cggcgagttc ccgaagctgc gggcgcgccg cccgttcctc gagcctcagc agctcctgct
     3781 ggggctcctc cgcgcgatgg ttttccctgg ccgcctgggg gtgctgaccc cgtccgagcg
     3841 ccacgtgccc cagaccaccg cccgctggcg cgcctcgggc ttcgacgccc acgtggctcc
     3901 gctgtcgccc tacgaggaga ccgaccccgc cgcggtccgc cgcgccgcgg acgcgctccg
     3961 ggccgggaac gcgggcctgg tggtgatgga ctgcatcggc ttccgccgca agacccggga
     4021 cgagatcgcg gcgctgaccg gcgcgccgac cctggtggcc aacctgctcg tggcccgcgt
     4081 ggccgcggag ctgctcggcc gctgatcccg tgacccgcgg cgcgcgtctc ctcctgctgg
     4141 ggccctacgt ggtcctgctg atggtgttct tcgccgcgcc gctcgtgctg atgttcgcga
     4201 tcagcctctc gcgccagtcg ttcggccagc tcgagtgggc cttcaccctg caccactacg
     4261 tccggttcct caccgacgcg tactacctgg gcgtgctcgc cgacaccctg tggctcgggg
     4321 tgctcaccac cgtggtcgcg ctgctcctgg gctatccgct ggcctaccac ctggccctga
     4381 cccgctcgcg ctggaagcca ctcctgatcg tcttcatcct gtcgccgctg ctggtgggca
     4441 tcgtcatccg ctgctacggc tggatgatcc tgctcgccga ccgtgggctg atcaacgaga
     4501 ccctcgcgca gcacggctgg atcaccaagc cgctcccgct catgtacaac aagttcgggg
     4561 tgaccatcgc gctcgtccac gtgttcctgc cgttcatggt cctctcgctc accggggtgc
     4621 tgaagcgcat cgagccccag ctgatcgagg cctcgcagac cctgggggcc tccccgcgcc
     4681 gggctttctt cgaagtgacc ctgccgctct cgctgcccgg catcctggcc ggctcgctgc
     4741 tcgtcttctc gctcgccatc agctccttcg tggtgccgat cctggtcggc gggttcaagg
     4801 tgcacgtgct gccgatggtg gtctacgagc aggtgctgtc ggtgttcgac tggcccttcg
     4861 gggccgccaa cgccttcgtg ctgctcgtga tctcggtggc cctgatcgcg gtctacatca
     4921 aggtgaccga gcgggcgctg cgcggaatcg tatgattctc aaaaccgtcg tggggctagt
     4981 ctacgcgttc ctgctgctgc cgatggtggt ggtggtgctg gccgcgttca acgcgggcaa
     5041 ctacttcacg ttcccgcccc agggcttctc cctcaagtgg ttcgcgaact tcttccagcg
     5101 gcgcgagttc atgcaggcgc tccggctctc caccgagctg gcggtctgga ccgcgctcgc
     5161 ctccaccgtg atcgggaccg cggcggccat cccgctggtg cggggccggt tccggggccg
     5221 ggatctgctc aacgcgttcg tgacctcgcc gctcctgctg ccgcagatcc tcaccggggt
     5281 cgcgctgctg cagttctaca ccctcttgaa gatgcaggcc tcctacgggg cgctcctcct
     5341 cggccacatc gtggtgacca cgccctacgt gatccgcacc gtcaccgcga ccctcaccca
     5401 cttcgacatg gcgctcgagg aggcggccca gagcctgggc gcgcacccgg tgcgcgcgtt
     5461 cttcgaggtg accctgggag tgatcaagcc gggggtggtg gcgggggcga tcttcgcctt
     5521 cgcgatctcg ttcgacaact tcaccctgtc gctcttcctg accagctcga agctgacccc
     5581 gctcccgatc gagctcttcg cctatctcaa gtactccttc gaccccaccg cggccgcggt
     5641 gtccgccttc gccatcgggg tggccctggt gctcgtcctg ggcatcgcgc gcttcatggg
     5701 gctcgaggag ttcaccggct tctgatggga cggacctgat ggagatcgcg cgatgagacc
     5761 ggttgccgcg gaggagatcg agagcctggc catcggcgcc tggatcctgg gcaccggcgg
     5821 cggcggcagc ccgtacctgg ccctgctcaa catgcgccag ctctatcgcc aggggatcgt
     5881 gagccagctc atggacgcgt ccgagctggc cgacgacgac ctggtcgcgg tggtctccaa
     5941 catgggcgcg ccgctcgtcg gccaggagcg cctcaccgat ccgaagacga tggccctggc
     6001 cgtcaccatg atggaggagt acctcgggcg gaagtttcgc gccctgatgt cgctcgagat
     6061 cggcggcggc aactccatcc agcccttcat gggcgcggcc atgctggatc tcccggtgat
     6121 cgacggcgac tgcatgggcc gcgcgttccc cgaggcccag atgaccagct tcgccatcca
     6181 cgacctccag atgtacccgc tgacgctcgc cgacgtgcgc gacaacgcgg tggtggtggc
//
LOCUS       DATMCV010000866         4536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_512742, whole genome shotgun sequence.
ACCESSION   DATMCV010000866 DATMCV010000000
VERSION     DATMCV010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512742"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VNU03_19880"
     CDS             complement(<1..435)
                     /locus_tag="VNU03_19880"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462701.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWP76789.1"
                     /translation="MTPSRDRVLSGMRPTGQLHLGNYLGALENWVKLQDQFDCFYFVA
                     NWHVLTTSPGDIQAVPGDTIEMGADWLGAGLDPERSTLFIQSLVPEHAELHLLFSMAT
                     PVTWLERVPTYKEMVEQLGIESPSYGLLGYPLLQAADILMYKA"
     gene            complement(442..1209)
                     /locus_tag="VNU03_19885"
     CDS             complement(442..1209)
                     /locus_tag="VNU03_19885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWP76790.1"
                     /translation="MSRLTVTVIAWNEEERLRACLESVAWADEIVVVDAESADKTVQI
                     AREFTDKVSVRVWPGFAIQKNFAIEQATGDWILSLDADERVTPELRERIRVILEEDGP
                     ADGFAIPRRNIFWGAWVRHGGLYPDYQLRLFRRRAGRFVENAVHESVTVTGRVDTLAE
                     PLLHHSYRDLEDFVRRSNRYSTLSAQDWIRRGKGAGLLGLVTRPLGRFFSMYIVHRGF
                     LDGWRGFVLAVLYGEYVFLRMAKVWETQRGPKPGGSD"
     gene            complement(1206..2282)
                     /gene="waaC"
                     /locus_tag="VNU03_19890"
     CDS             complement(1206..2282)
                     /gene="waaC"
                     /locus_tag="VNU03_19890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HWP76791.1"
                     /translation="MTAPGASAREPGRISGPALLKIAIVKLSSLGDVIHALPVARALR
                     RARPGLHLTWIVEAREYALLNGHPDLDVVVPVDTRLWRRLIWRPAGARQVLGKVRRLK
                     SRVRAARFDVAIDLQGLMKSGLLTAYTGAPVRIGFTADRCRERLNCLFTNRRVRPPAS
                     AVHVVDQYLSLLEPLGVGPGPVEFHVPIPAAAERRMEDFLGEQGVKSRDSLVAINPGA
                     GRADKQWPVAHFAALAEQLAQEPNTRVLLLWGPDEVHMARQIRDGMQARAILAPPTDL
                     HELGALLRRSSLMVANDTGPLHLAAALGTPSLGLFGPTRAERNGPYGPHCRGVQSSNG
                     TMAGLLPSVAVEAARALLAARSDR"
     gene            complement(2279..2770)
                     /locus_tag="VNU03_19895"
     CDS             complement(2279..2770)
                     /locus_tag="VNU03_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HWP76792.1"
                     /translation="MAAVVSVDEAGRLAAGWRAEGKRLVLANGCFDLLHVGHVRYLQA
                     ARALGDALVVGLNSDASVRRLKGPGRPVMPAVERAELLAAVSGVDAVVVFEEDSADAL
                     IERLRPDVHAKGTDYTEQSVPERATVAAVGATVAIAGDPKSHSTKDLIATILARFGRT
                     PSR"
     gene            complement(2748..3764)
                     /locus_tag="VNU03_19900"
     CDS             complement(2748..3764)
                     /locus_tag="VNU03_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWP76793.1"
                     /translation="MFPLKRLQAALTRLAGRRVVVVGDLIADEYLYGKPARISREAPV
                     LILRFTDREVRLGGAANAAHNVHALGATVLPVGLLGQDAAGDEVHALFRQAGIGTDGV
                     SREAGRPTPVKTRIMAGGYQATRQQVVRLDREPEGGIAGPAEARLIETLRAVGATADG
                     FLVSDYGYGTVTPRIYETVLELARARKAPVSIDSRYELPRFRGATAATPNEPEVEALT
                     GHELTDDAAVEKAGRVVLERLDARLLLLTRGSRGMALFEREGPVTFMPIHGTDEIADV
                     TGAGDTVISTFTVALAAGTAPGEAAWLANVAGGVVVMKRGTATVTVPELAAALASDGG
                     RRQR"
     gene            complement(3766..4335)
                     /locus_tag="VNU03_19905"
     CDS             complement(3766..4335)
                     /locus_tag="VNU03_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP76794.1"
                     /translation="MDRDGCLTEEVGYVNHPTRIRLLPRTAEAVRRLNQAGVPAVMVT
                     NQTGIARGYFSEETLHATNARMQGALAAAGARLDGAYVCMHHPSEGQPPYRADCDCRK
                     PRPGLLHRAAAELDLDLTASVMVGDKFSDVAAGHAVGAVGVLVLTGYGRGEWEYHRHG
                     QPVRPDHVAEDLLDAVEWALARTASGTRA"
BASE COUNT          607 a         1663 c         1656 g          610 t
ORIGIN      
        1 cgccttgtac atgaggatgt cggccgcctg gagcagcgga tagccgagca ggccgtagga
       61 cggcgactcg atgccgagct gctccaccat ctccttgtag gtcgggaccc gctcgagcca
      121 ggtcaccggc gtcgccatcg agaagagcag gtgcagctcc gcgtgctccg gcaccaggga
      181 ctggatgaag agggtcgacc gctccgggtc gagcccggcc cccagccagt ccgcgcccat
      241 ctcgatggtg tcgcccggca ccgcctggat gtcgcccggg ctggtggtca gcacgtgcca
      301 gttggccacg aagtagaagc agtcgaactg gtcctggagc ttgacccagt tctcgagcgc
      361 ccccaggtag ttcccgaggt ggagctggcc ggtcggtcgc attcccgaca gcacgcggtc
      421 ccgcgaaggc gtcatgatct cctagtcact ccctccgggc ttcggccccc gctgcgtctc
      481 ccacaccttg gccatgcgca ggaacacgta ctcgccgtac aggaccgcca gcacgaaccc
      541 ccgccagccg tccaggaagc cccggtgaac aatgtacatg gaaaagaagc gtcccagcgg
      601 acgagtcacg aggccgagca ggcccgcccc cttcccccgc cggatccagt cctgggccga
      661 gagggtcgag tagcggttgg agcggcgcac gaagtcctcg aggtcgcgat aggagtggtg
      721 gagcagcggc tccgcgagcg tgtccacccg tccggtcacc gtcaccgact cgtggaccgc
      781 gttctccacg aagcgccccg cccgccgccg gaagagccgg agctggtagt cggggtagag
      841 cccgccgtgg cgcacccagg cgccccagaa gatgttgcgg cgcgggatgg cgaagccgtc
      901 ggcgggcccg tcctcctcga ggatgacccg gatgcgctcg cgcagctccg gggtcacccg
      961 ctcgtcggcg tcgagggaga ggatccagtc cccggtggcc tgctcgatcg cgaagttctt
     1021 ctggatcgcg aagccgggcc acacgcgcac cgacaccttg tcggtgaact cgcgggcgat
     1081 ctgcaccgtc ttgtcggcgg actccgcgtc gaccaccacg atctcgtcgg cccacgcgac
     1141 gctctcgagg cacgcgcgca gccgctcctc ctcgttccag gcgatcacgg tcacagtgag
     1201 ccggctcacc ggtcgctccg cgccgcgagg agcgcccgcg ccgcctcgac cgcgaccgac
     1261 gggaggagac cggccatggt gccgttggag ctctgcactc cgcggcagtg cgggccgtag
     1321 gggccgttgc gctccgcccg ggtggggccg aagaggccga ggctcggcgt gccgagcgcg
     1381 gcggccaggt gcagcgggcc ggtgtcgttg gccaccatca gcgacgaccg gcgcagcagc
     1441 gcgcccagct cgtggaggtc ggtggggggc gccaggatcg cgcgcgcctg catgccgtcc
     1501 cggatctgcc gcgccatgtg gacctcgtcg ggcccccaca gcagcaggac gcgggtgttc
     1561 ggctcctggg ccagctgctc ggccagcgcg gcgaagtgcg cgaccggcca ttgcttgtcg
     1621 gcgcggccgg ctcccggatt gatcgcgacc aggctgtcgc ggctcttcac cccctgctcg
     1681 cccaggaagt cctccatccg gcgctccgcc gcggccggga tcggcacgtg gaactcgacc
     1741 gggccggggc cgacgccgag cggctcgagc agcgacaggt actggtccac cacgtgcacc
     1801 gcgctcgcgg gcgggcgcac gcgccggttg gtgaagagac agttgaggcg ctcgcggcag
     1861 cggtccgcgg tgaagccgat gcgcaccggc gcgccggtgt aggcggtgag cagcccgctc
     1921 ttcatcagcc cctgcaggtc gatggccacg tcgaaccggg ccgcgcggac gcggctcttg
     1981 agccgtctca ccttgcccag cacctggcgc gccccggcgg gccgccagat gagccggcgc
     2041 cagagccggg tgtcgaccgg taccaccacg tccaggtccg gatggccgtt gagcagcgcg
     2101 tactcgcgcg cctccacgat ccacgtgagg tggagccccg gccgcgcgcg gcgcagcgcg
     2161 cgcgccaccg gtagcgcgtg gatgacgtcg ccgagcgagg acagcttcac gatggcgatc
     2221 ttcaggagcg cgggccccga gatgcgcccc ggctccctcg cgctcgcacc cggagcggtc
     2281 atcgggaggg cgtccggccg aagcgggcca ggatggtggc gatgaggtcc ttggtcgagt
     2341 ggctcttcgg atcgcccgcg atggccacgg tggctcccac cgcggccacc gtcgcgcgct
     2401 cgggcacgct ctgctccgtg tagtcggtgc ccttggcgtg cacgtcgggg cgcagccgct
     2461 cgatgagggc gtcggcgctg tcctcctcga agacgaccac cgcgtcgacg ccgctcaccg
     2521 cggccaggag ctccgcgcgc tccaccgccg gcatcaccgg gcggcccggc cccttgagcc
     2581 ggcgcaccga ggcgtccgag ttgagcccga ccaccagcgc gtcgccgagg gcgcgggcgg
     2641 cctgcaggta gcgcacgtgg cccacgtgca ggaggtcgaa gcagccgttg gcgaggacca
     2701 ggcgcttgcc ctcggcgcgc cagccggcgg cgagacggcc cgcctcgtca acgctgacga
     2761 cggccgccat cgctcgcgag cgcggccgcc agctcgggga cggtcacggt ggcggtgccg
     2821 cgcttcatga ccaccacgcc gccggccacg ttggcgagcc acgccgcctc gcccggcgcg
     2881 gtgcccgcgg ccagcgccac cgtgaaggtg ctgatcacgg tgtcacccgc gccggtcacg
     2941 tccgcgatct cgtcggtgcc gtggatcggc atgaaggtga ccggcccctc ccgctcgaag
     3001 agcgccatgc cgcggctgcc ccgcgtgagc aggaggagcc gcgcgtcgag ccgctcgagc
     3061 accacgcgtc ccgccttctc gaccgccgcg tcgtcggtca gctcgtgccc ggtgagcgcc
     3121 tccacctcgg gctcgttcgg ggtcgccgcg gtggcgcccc ggaagcgcgg cagctcgtag
     3181 cggctgtcga tcgacaccgg cgccttgcgc gcgcgggcga gctcgagcac cgtctcgtag
     3241 atccgcgggg tcaccgtgcc gtagccgtag tcggagacga ggaagccgtc cgcggtggcg
     3301 cccaccgcgc gcagcgtctc gatcagccgc gcctcggccg gccccgcgat gccgccctcc
     3361 ggctcgcggt cgaggcggac cacctgctgc cgggtggcct ggtagccgcc ggccatgatg
     3421 cgcgtcttca ccggcgtggg gcgtcccgcc tcacgggaga cgccgtcggt gccgatgccc
     3481 gcctggcgga agagcgcgtg cacctcgtcg cccgcggcgt cctgtccgag cagacccacc
     3541 ggcagcacgg tggcgccgag cgcgtggacg ttgtgggccg cgttggccgc gccgcccagc
     3601 cgcacctcgc gatcggtgaa gcgcaggatg aggaccgggg cctcgcggga gatgcgcgcg
     3661 ggcttgccgt agagatactc atcggcgatg agatcgccca ccaccaccac gcggcggccg
     3721 gcgaggcggg tcagcgcggc ctggagtcgc ttgaggggga acacgtcagg cccgcgtgcc
     3781 gctcgcggtg cgggcgagcg cccactccac cgcgtcgagg agatcctcgg ccacgtggtc
     3841 cggcctcacg ggctgcccgt ggcggtggta ctcccactcc ccgcggccgt agccggtcag
     3901 cacgagcacg cccaccgcgc ccaccgcgtg cccggccgcc acgtcggaga acttgtcgcc
     3961 gaccatcacc gaggcggtca ggtcgaggtc gagctcggcc gccgcgcgat gcagcaggcc
     4021 cgggcgcggc ttgcggcagt cgcagtcggc gcgatacggc ggctggccct cgctcgggtg
     4081 gtgcatgcac acgtaggcgc cgtcgagccg cgcgcccgcc gcggccagcg ctccctgcat
     4141 gcgcgcgttg gtggcgtgca gggtctcttc cgagaagtag ccccgcgcga tgccggtctg
     4201 gttggtgacc atcaccgcgg ggacgccggc ctggttcagg cgccggaccg cctccgcggt
     4261 gcgcggcagg aggcggatcc gggtcgggtg gttgacgtag cccacctcct cggtgaggca
     4321 gccgtcccgg tccatgaaca cggccggtcg tggcgcacgg cgctcgggcg acattgtctc
     4381 gcagtgtaat gcgctcgccc tcgaatgatc aatgaaaatc gggggtttga cgcggccggc
     4441 gcggatggtc agcggcgcag gagctcgtag agcgccgtga aggcccgcga ctgcgccgcg
     4501 taggtgaagc cctcgacgga ggcgcgggcc cggctg
//
LOCUS       DATMCV010000867         2611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_246805, whole genome shotgun sequence.
ACCESSION   DATMCV010000867 DATMCV010000000
VERSION     DATMCV010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246805"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2611
                     /locus_tag="VNU03_19910"
     CDS             <1..>2611
                     /locus_tag="VNU03_19910"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HWP76795.1"
                     /translation="KTQALLAYLALPLGQAHSRDKLATLLWGDMPDAQARGNLRHALS
                     RIRKALPAGARHGLILDGPTVALDPSVVDVDVAQFERLVADGQPPALEQIGGLYRGDL
                     LAGLALTERPFEEWLTSERERLHELAIQGLGRLLTHQQRGGAAEPAIQTGLRLLALDP
                     LQEPVHRTLMRLYARLGRREAALRQYQLSVDALKRELNTSPEAETTQLYQEILRSRSR
                     RPDRATVAGSAGEDPAPGPAADLLSAAVAALPEPPMNLPAPTSELIGRADALAEVTEL
                     LGVHRLVTLIGAGGIGKTRLGLEVARQVLPGFADGVWVAELAPLSDPGLVPATVAVAL
                     GLTLLAGAESPERVAAALGAKRALLMLDNCEHVIEAAARMAEALLRANPHVRVMATSR
                     EPLRAPGEYVYRVLPLEVPAEGAEGREDLLEAAAVQLFVARAQAVEHFSLDVRTAAIT
                     GAVCRRLDGIPLAIELAAARTATLGLEELAAHLDDRFRLLTGGHRTALPRHQTLRATL
                     DWSHELLSAIERTALRRLAIFVGGFTLEAASAVATASDLGAPEVVDSVTNLAAKSLVV
                     VEVAGAVTRYRLLETTRAYALEKLTASGELEQAARRHAEYCRDLFERAEVEWETRTTV
                     EWVAAYGRQIDNVRAALDWAFSPSGDASIGAALTAASVPLWFQHSLMVECRGRVERAL
                     ASLEPRPGRGGRREMQLFAALGVSLMQTKGPAPDTTAAWTTALEIAERVDDIEYQLRA
                     LWGLWHFRVSRGECRAALALAESFCGQAAGNDNPAELLVGERMVGVSLHYLGDQANAR
                     LHLERMLGRYVAPAHRSDTIRFQYDQQVAGRMILARILWLQGFPDQALRTAQENVEDA
                     RAIDHALSLCYALE"
BASE COUNT          342 a          965 c          935 g          369 t
ORIGIN      
        1 aagacccagg ctctcctggc ctacctggcg ctccccctcg ggcaagccca ttcgcgcgac
       61 aagctcgcga ccctgctctg gggagacatg ccggatgccc aggcccgggg caatctacgc
      121 cacgccctgt cgcgcatccg caaggccctc cccgccggcg cccgacacgg cctcatcctc
      181 gacggcccga cggtggccct cgatccctcg gtcgtggacg tcgacgtcgc ccagttcgag
      241 cggctggtcg ccgacggcca gcctcctgcg ctcgagcaga tcggtggcct ctatcggggg
      301 gacctgctgg ccggcctggc gctcaccgag cggcccttcg aggagtggct cacctccgag
      361 cgggagcgcc tgcacgagct ggccatccag ggcctggggc gcctgctcac ccaccagcag
      421 cggggcggcg ccgccgagcc ggccatccag accgggctcc ggctcctcgc cctggacccg
      481 ctccaagagc ccgtccaccg cacgctcatg cggctctatg cccggctcgg gcgccgggag
      541 gccgcgctgc gccagtacca gctcagcgtg gatgccctca agcgcgagct caacacgtcg
      601 cccgaggcag agaccaccca gctgtaccag gagatcctgc gctcgcgatc ccgccgtccc
      661 gaccgggcca cggtagccgg ctcggcgggc gaggaccccg cgccaggccc cgcggccgat
      721 ctcctgtccg ccgcggtggc ggccctgccg gagccgccaa tgaacctgcc ggcgccgacc
      781 tcggagctga tcggccgggc ggacgcgctc gcggaggtga cggagctgct gggggtccac
      841 cggctggtca ccctgatcgg ggccgggggc atcggcaaga cgcggctggg cctggaggtc
      901 gcgcgtcagg tgctgccggg tttcgccgac ggggtctggg tcgccgagct cgcgccgctg
      961 tccgatcccg ggctggtgcc ggccaccgtc gcggtcgccc tcgggctcac gctcctcgcc
     1021 ggcgcggagt cccccgagcg ggtggcggcg gcgctcggcg ccaagcgcgc cctgctcatg
     1081 ctggacaatt gcgagcacgt gatcgaggcg gcggcccgca tggccgaggc gctgctgcgc
     1141 gccaatccgc atgtccgcgt gatggcgacc agccgcgagc cgttacgcgc gccgggggag
     1201 tacgtgtacc gggtgctccc cctcgaggtg ccggcggagg gagccgaggg gcgcgaggat
     1261 ctgctggaag cggccgcggt ccagctgttc gtggcgcgcg cgcaggcggt cgagcacttc
     1321 tcgctggacg tgcgcaccgc ggcgatcacc ggcgcggtct gtcggcgcct cgacggcatc
     1381 ccgctcgcga tcgagctggc ggcggcccgc accgccacgc tcgggctgga agagctcgcc
     1441 gcccatctgg acgatcgctt ccgactgctc accggcggcc atcgtaccgc gctgccgcgg
     1501 caccagacgc tgcgcgcgac gctcgactgg agccacgagc tgctctccgc gatcgagcgc
     1561 accgccctgc gtcgcctggc catcttcgtc gggggcttca cgctggaggc ggcgagtgcc
     1621 gtcgcgacgg cctccgacct cggcgcgcca gaggtcgtgg acagcgtcac gaatctggcc
     1681 gcgaagtcac tcgtggtcgt ggaggtcgcg ggcgcggtca cgcgataccg gctgctcgag
     1741 acgacgcggg cctacgcgct cgagaagctc accgccagcg gcgagctcga gcaggccgcg
     1801 cgacgccacg ccgaatactg ccgtgacctc ttcgagcggg ccgaggttga atgggagacg
     1861 cggaccactg tcgagtgggt cgcggcgtac ggccgccaga tcgacaacgt tcgggcggcg
     1921 ctggactggg cgttttcgcc gagcggcgac gcgtcgatcg gggcggcgct caccgccgca
     1981 tcggtgcccc tgtggttcca gcactcactg atggtcgagt gtcgcgggcg cgtcgaacgc
     2041 gcgctcgcca gcctcgaacc ccgcccgggt cgaggcgggc gccgcgagat gcagctcttc
     2101 gctgccctcg gcgtgtcgct catgcagacg aagggccccg cgcccgatac caccgcagcc
     2161 tggacgaccg cgctcgagat cgccgaacgc gtcgacgaca tcgagtatca gctgcgggcc
     2221 ctttggggcc tgtggcattt ccgcgtcagc cgcggcgagt gtcgcgccgc gctggcgctg
     2281 gcggaaagct tctgcggcca ggccgcaggc aatgacaatc cggccgagct gctcgtcggc
     2341 gagcggatgg tcggcgtctc cctccattac ctgggagatc aggcgaacgc gcggcttcac
     2401 ctcgagcgca tgctcggtcg ctatgtcgcc ccggctcacc ggtcggacac catccgcttt
     2461 caatacgacc agcaggtggc gggccgcatg atcctggcgc ggatcctctg gttgcaggga
     2521 ttccccgatc aggctctgcg gacggcccag gagaacgtcg aggacgcgcg ggcgatcgat
     2581 catgcgctct cgctgtgcta cgcgctggag g
//
LOCUS       DATMCV010000868         4100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_778634, whole genome shotgun sequence.
ACCESSION   DATMCV010000868 DATMCV010000000
VERSION     DATMCV010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778634"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VNU03_19915"
     CDS             <1..1122
                     /locus_tag="VNU03_19915"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWP76796.1"
                     /translation="EKAPSVDDPEWHRVFAPGGAFPRTGDCFRQPDLARTIAELGQEP
                     ELFYKGRVARSIVKRMSRDGFLTAEDLAAHEGEWGEPISSTYRGYTVYETPPPTQGLA
                     ALLSLNLLEGFDLARYEPHSPEHLHLLIEMTKLAYADRDRWVADPTRERVPVEALLSK
                     SYAARRRRDFDPEKAQWHKWGELDGDTTGFVVADARGNIMSVIQSLYKSFGSGVVAPD
                     TGVVLQNRGAYFNTDPAHPNHFAPGKRPFHTLIACVVTRDERPVLGYSNMGGDGQAMF
                     HVQSLTNALDYGMEIQEAIERPRFVAGPIDPGDVTDQVRIESRVPAATREALERKGHH
                     VVPVSDFFMRMGHAHGITLTDGTLRGGADPRGDGAAIGF"
     gene            1122..1628
                     /locus_tag="VNU03_19920"
     CDS             1122..1628
                     /locus_tag="VNU03_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76797.1"
                     /translation="MAGRRALLAAVAMAGLLLAGEPGAAEEWGAIEPGVTTAEQVRAR
                     YGAPSKETRGKADGYDIMQWVYEGAQVPGGIQKMTVDYGLLTPQGFKPTVVRVLRLEP
                     KAKVFGRNTVIQAWGVPDGITQQNELDTFFYKTGLLVNFDKEGDEAALLTFTPPQPDA
                     PPPAAPKR"
     gene            1648..2538
                     /locus_tag="VNU03_19925"
     CDS             1648..2538
                     /locus_tag="VNU03_19925"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HWP76798.1"
                     /translation="MIDHLMALDPGGRGIAAFFVRGGATAAAQALRKSRRVLLTTGFA
                     VGPGLPETDGPPGTACLGRALRTLGAEVVYVTDAVAVPPLQAALKVLGEPSTVETFHV
                     AHGAGGGPAAGGARETARRLLAEHRPTHLVAIERPGRSRDGEYRSARGRSLTEWNGAL
                     DELFLAAPARIVTVGVGDGGNEVGMGTVRARVARAGGVFAQIASVVKVKHLVVAGVSN
                     WGAYGIVAELSRLTGRALLHTGEEEQAMVQACVDAGAVDGLTHRPEPTVDGLPLPAHI
                     GMLELLRVLESSRRTGGPSK"
     gene            2535..3872
                     /locus_tag="VNU03_19930"
     CDS             2535..3872
                     /locus_tag="VNU03_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWP76799.1"
                     /translation="MSAAHASVIDTYKAMHPKSSALYERARGIIPGGVTHDGRHMKPF
                     PVYVERALGSHKWDVDGHEYIDYWMGHGALFLGHCHPAVVKAIQEQAARGTHFGASHE
                     LEVRWAELIAKLIPSAQMVRFAMSGTEATHLALRIARAFTGRNKVVKFQGHFHGWHDG
                     VVAAVNPPYDVPMSAGVPESVLDQLLVCPPNDIKSVDTMLERGDVAAVILEPAGGQSG
                     TTPTIPGYLQELRSLTTRHNVVLIFDEVITGFRYSPGGAQAYFGVTPDMTTLAKIVAG
                     GLPGAAIVGKKELMSMMAHRGEPVFDRSQRVAQNGTFNSNPVCAAAAIATLEMVSDGS
                     LHARANKAGDELRSGLSDAMKRAGVPGTCFGEASIFHVSFEGKPGLAGFDRPRRGDLY
                     HLLRCALLNNGVDCASHHGWISAVHSDTDIERTIAAHEKAFREMAADGVFKGM"
BASE COUNT          610 a         1484 c         1447 g          559 t
ORIGIN      
        1 gagaaggcgc cctccgtcga cgacccggag tggcatcgcg tcttcgcgcc gggcggcgcg
       61 ttcccccgca ccggcgactg cttccgccag cccgacctgg cccgcaccat cgccgagctg
      121 gggcaggagc cggagctgtt ctacaagggg cgggtcgcgc gctcgatcgt gaagcgcatg
      181 agccgcgacg gcttcctcac cgcggaggac ctggccgcgc acgaggggga gtggggcgag
      241 cccatctcgt ccacctatcg cggctacacg gtgtacgaga cgccgccgcc cacccagggc
      301 ctggccgcgc tgctgagcct gaacctgctc gagggcttcg atctcgcgcg ctacgagccg
      361 cactcgcccg agcaccttca cctgctgatc gagatgacca agctggccta cgccgaccgc
      421 gaccgctggg tggccgaccc cacgcgcgag cgcgtgccgg tggaggccct gctcagcaag
      481 agctacgcgg cgcgccgccg ccgcgacttc gatcccgaga aggcgcagtg gcacaagtgg
      541 ggcgagctcg acggcgacac caccggcttc gtggtggcgg acgcgcgggg caacatcatg
      601 agcgtcatcc agagcctgta caaatcgttc ggctccgggg tggtggcccc cgacaccggg
      661 gtggtgctgc agaaccgggg cgcctacttc aacaccgatc ccgcgcaccc gaaccacttc
      721 gccccgggca agcggccgtt ccacaccctg atcgcctgcg tggtcacgcg cgacgagcgg
      781 ccggtgctgg gctactccaa catgggcggt gacgggcagg cgatgttcca cgtccagtcg
      841 ctcaccaacg cgctcgacta cggcatggag atccaggagg ccatcgagcg gccccgcttc
      901 gtggccggcc caatcgaccc gggcgacgtg acggaccagg tgcgcatcga gagccgcgtc
      961 cctgccgcca cccgggaggc gctcgagcgc aagggccacc acgtcgttcc cgtgtcggac
     1021 ttcttcatgc gcatgggcca cgcccacggc atcaccctca ccgacggcac cctgcgcggc
     1081 ggcgccgatc cccgcggcga cggcgccgcg atcggcttct gatggccggg cggcgcgcgc
     1141 tcctcgccgc ggtcgcgatg gccgggctgc tcctggccgg ggagccgggc gcggcggagg
     1201 agtggggcgc gatcgagccc ggcgtgacga ccgccgagca ggtgcgcgcg cgctacggcg
     1261 cgcccagcaa ggagacccgc ggcaaggcgg atggctacga catcatgcag tgggtgtacg
     1321 agggcgcgca ggttccgggt ggcatccaga agatgaccgt ggattacggg ctgctcaccc
     1381 cgcagggttt caagcccacg gtggtgcggg tgctccgcct cgagccgaag gcgaaggtct
     1441 tcggccggaa cacggtgatc caggcctggg gggtgcccga cggcatcacc cagcagaacg
     1501 agctggacac cttcttctac aagaccggcc tgctcgtgaa cttcgacaag gagggcgacg
     1561 aggccgcgct gctgaccttc acgccgcccc agcccgatgc cccgccgccc gcggctccga
     1621 agcggtagcc cggcgccgcg tactccgatg atcgaccacc tgatggcgct cgatccgggc
     1681 ggccgcggca tcgccgcgtt cttcgtgcgc ggcggtgcca ctgcggcggc ccaggcgctg
     1741 cgcaagagcc ggcgcgttct gctgaccacc ggcttcgcgg tgggcccggg cctgcccgag
     1801 accgacggcc cgcccggcac cgcgtgtctc ggccgtgcct tgcggaccct gggcgccgag
     1861 gtggtctacg tgaccgacgc ggtcgcggtg ccaccgctgc aggccgcact caaggtgctg
     1921 ggcgagccct cgacggtcga gacctttcac gtggcgcacg gtgcgggcgg tgggcccgcc
     1981 gcgggcggag cacgcgagac cgcacggcgc ctgctggccg agcaccggcc cacgcacctg
     2041 gtcgccatcg agcggccggg ccgctcgcgc gacggcgagt atcggtccgc ccgcggccgc
     2101 tcgctcaccg agtggaacgg cgccctcgac gagctgttcc tggccgcgcc cgcccgcatc
     2161 gtgaccgtcg gtgtgggcga cggcgggaac gaggtgggca tgggcaccgt ccgcgcccgg
     2221 gtggcccggg cgggcggcgt cttcgcccag atcgcctcgg tggtgaaggt gaagcacctg
     2281 gtggtggccg gcgtgtccaa ctggggcgcc tacggcatcg tggccgagct gtcccggctg
     2341 accggccgcg ccctgctgca caccggcgaa gaggagcagg ccatggtaca ggcctgcgtg
     2401 gatgcggggg cggtcgacgg cctgacccat cgccccgagc ccacggtgga cggcttgcct
     2461 ttgcccgccc acatcggcat gcttgagctg cttcgagtgc tggaatcatc ccgtcgaacc
     2521 ggaggcccga gcaaatgagt gctgcccacg cgagcgtgat cgacacctac aaggccatgc
     2581 accccaagtc gtccgcgctc tacgagcgcg cgcgcggcat catccccggc ggcgtcaccc
     2641 acgacggccg gcacatgaag ccgttcccgg tgtacgtcga gcgggcgctc gggtcgcaca
     2701 agtgggacgt cgacgggcac gagtacatcg actactggat gggacacggc gcgctcttcc
     2761 tggggcactg ccatcccgcg gtggtcaagg ccatccagga gcaggccgcg cgcggcaccc
     2821 acttcggagc gagccacgag ctggaggtac gctgggccga gctgatcgcc aagctgatcc
     2881 cgtcggccca gatggtgcgc ttcgcgatgt ccggcaccga ggccacgcac ctggcgctgc
     2941 gcatcgcgcg cgccttcacc gggcgcaaca aggtggtgaa gttccagggc cacttccacg
     3001 gctggcacga cggggtggtc gcggcggtga acccgcccta cgacgtgccg atgtcggccg
     3061 gcgtgccgga gtcggtgctc gaccagctgc tggtgtgccc gccgaacgac atcaagtcgg
     3121 tggacaccat gctcgagcgc ggcgacgtgg ccgcggtgat cctggagccg gccggcggcc
     3181 agtccggcac cacgcctacc attccgggct acctccagga gctgcgcagc ctcaccactc
     3241 gccacaacgt ggtgctgatc ttcgacgagg tgatcaccgg cttccgctac tcgccgggcg
     3301 gcgcccaggc ctacttcggc gtcacgccgg acatgaccac gctggccaag atcgtggccg
     3361 gcgggctgcc cggcgccgcg atcgtcggca agaaggagct gatgtcgatg atggcccacc
     3421 gcggcgagcc ggtcttcgac cggagccagc gcgtggccca gaacggcacg ttcaactcca
     3481 acccggtctg cgccgccgcc gccatcgcca ccctcgagat ggtgagcgac gggtcactgc
     3541 acgcgcgcgc caacaaggcg ggggacgagc tgcgctcggg cctctcggat gcgatgaagc
     3601 gcgccggcgt gcctggcacc tgcttcggcg aggcctcgat cttccacgtc tcgttcgagg
     3661 gcaagcccgg cctggccggc ttcgaccgcc cgcgccgcgg cgatctctac cacctgctgc
     3721 gctgcgccct gctcaacaac ggggtcgact gcgccagcca ccacggctgg atctcggcgg
     3781 tgcacagcga caccgacatc gagcgcacca tcgcggccca cgagaaggcg ttccgcgaga
     3841 tggccgccga cggtgtcttc aagggcatgt aaccacctgc cttcccctct cccctcaggg
     3901 gagagggcag ggtgaggggg tggttttcca gagaggaacg cagtggacga cgcccggcgc
     3961 cgcgccaagc tccaggatct ctacgacctc gcccagggct ccgaggagtt cgagggcggc
     4021 atcacctggg agcccgacac cgaggccctc gtggtgggca actgggcctt cttcgccatc
     4081 gacgagatcg gcgacctggc
//
LOCUS       DATMCV010000869         3442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_451559, whole genome shotgun sequence.
ACCESSION   DATMCV010000869 DATMCV010000000
VERSION     DATMCV010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451559"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VNU03_19935"
     CDS             <1..309
                     /locus_tag="VNU03_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76800.1"
                     /translation="HTITFAFGAGLAAAAGALLGPVFLLYPSMGDLASLKAFSVVILG
                     GLGNFAGAALGGIVLGVAEELGAGYVSSGYRDAVGFLMIVAVLLLRPSGLFARAERVG
                     "
     gene            306..1271
                     /locus_tag="VNU03_19940"
     CDS             306..1271
                     /locus_tag="VNU03_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP76801.1"
                     /translation="MSRLALLIGLVVAATAPAWVWNPYHLHTLIMAGIFAVLALSLNL
                     LLGYTGQLSLGHAAFFGIGAYATGLLTVKLEWSAWIGLLAAIVLPAVAGYVIGRLALK
                     LRGAYFVLLTISFAGCVSLVSVNWMDLTNGSLGLPGVPPLEIALPGLPALSLRTKSAF
                     YYVVLAAVVLSYLVCLALIRSRVGRALVALRENETLAASVGIDGTHYLVLAAVVSAAM
                     AGFGGGLYAHYTRFVSPEVFLFTYTVTMVIMVVAGGKGTLAGPIVGAVLFTVLPEALR
                     AATSWQWQMLLYGILLVGVLFFMPEGIVPALRGLGPAARRRWSPS"
     gene            1268..2038
                     /locus_tag="VNU03_19945"
     CDS             1268..2038
                     /locus_tag="VNU03_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76802.1"
                     /translation="MTLEAHDLTVRFGGVAALTGVSFTVAAGTVTSLIGPNGAGKTTA
                     FNVITGFQRPTQGTVRHDGQPITGWRPHRIAARGLVRTFQKTSVFPGLSVRENVLTGL
                     HLRGRVGVAAALLTRGRVRAEERRLEAEAERVIDFVGLGSRRGEAANGLSYGDQRLLE
                     LAVALAAQPRLLMLDEPASGMSASEKVTVAALIGRIREQGITVFLVEHDMRLVMGISD
                     RVLVLNYGRLIADGDPAAVQRDPEVVRAYLGSGAHAHA"
     gene            2025..2729
                     /locus_tag="VNU03_19950"
     CDS             2025..2729
                     /locus_tag="VNU03_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76803.1"
                     /translation="MLTLERVAAGYGRITALHGVSLTVGAAEFVCLIGANGAGKSTTL
                     KTISGLVRAGAGRIVFDGQEIQRLAPQDILRRGIAHCPEGRRVFPHMTVEENLQMGAY
                     VRTDGVAVAADRERVFGHFPILADRRRQAAGTLSGGEQQMLAIGRCLMARPRLILFDE
                     PSLGLAPTVTETVFQIIAGIQREGTTVLMVEQNAYMALQLAGRAYVMETGRITLEGPA
                     KDLMRNDHVKKAYLGG"
     gene            2985..>3442
                     /locus_tag="VNU03_19955"
     CDS             2985..>3442
                     /locus_tag="VNU03_19955"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76804.1"
                     /translation="MGAKYFGASVKRKEDPRYLRGEGRFVDDIKLPGMLHAAFVRSPY
                     AHARITAIRTEAAKRLPGVAHVFTFADLERWMKPLPLFGAIPPGLSARVAVMMRQIGQ
                     LALCRDEARHVGEIVAMVLASSRALAEDGCELVEVDYEPLPVLADAVAAA"
BASE COUNT          439 a         1216 c         1250 g          537 t
ORIGIN      
        1 cacaccatca ccttcgcgtt cggggccggc ctggccgccg cggcgggggc cctgctgggt
       61 cccgtgttcc tgctctaccc ctccatgggc gacctggcct cgctcaaggc cttctcggtg
      121 gtcatcctgg gcggcctcgg caacttcgcg ggcgccgcgc tcggcggcat cgtcctgggc
      181 gtggcggagg agctgggcgc gggatacgtc tcctccggct accgcgacgc ggtgggcttc
      241 ctgatgatcg tggcggtgct gctgctgcgt ccctccgggc tcttcgcccg cgccgagcgg
      301 gtgggatgag tcgcctcgcg ctcctgatcg ggctggtggt ggcggcgacg gcgccggcgt
      361 gggtctggaa cccgtaccac ctgcataccc tgatcatggc cggcatcttc gcggtgctcg
      421 cgctcagcct gaatctcctg ctgggctaca ccggccagct ctcgctcggc cacgccgcct
      481 tcttcggcat cggcgcctac gccaccgggc tcctcaccgt gaagctcgag tggtcggcct
      541 ggatcggcct gctcgccgcg atcgtgctgc cggcagtggc gggctacgtg atcggccggc
      601 tcgcgctcaa gctgcgcggc gcctacttcg tgctgctgac catctccttc gcggggtgcg
      661 tctcgctggt cagcgtgaac tggatggacc tcaccaacgg ttcgctcggc ctgcccggag
      721 tgcccccgct cgagatcgcc ctgcccgggc tgcccgcgct ctcgctgcgc accaagagcg
      781 ccttctacta cgtggtgctg gccgcggtcg tgctctcgta cctggtgtgc ctggccctga
      841 tccgctcgcg ggtggggcgg gcgctggtgg cgctccggga gaacgagacg ctcgccgcct
      901 cggtcggcat cgacggcacg cactacctgg tgctggccgc ggtggtgagc gcggcgatgg
      961 ccggcttcgg cggcggcctc tacgcgcact acacgcgctt cgtgagcccg gaggtcttcc
     1021 tcttcaccta caccgtcacg atggtgatca tggtggtggc gggcgggaag ggcacgctgg
     1081 ccggccccat cgtgggcgcg gtgctcttca cggtgctgcc ggaggcgctg cgggccgcca
     1141 cctcctggca gtggcagatg ctgctctacg gaatcttgct ggtgggcgtg ctgttcttca
     1201 tgcccgaggg aatcgtgccg gccctgcgcg ggctcggccc ggccgcccgg cgccgctgga
     1261 gcccgtcgtg accctcgagg cccacgacct cacggtgcgc ttcggcgggg tggccgcgct
     1321 cacgggggtg agcttcacgg tcgcggccgg caccgtgacc agcctgatcg gcccgaacgg
     1381 cgcgggcaag accaccgcct tcaacgtgat caccggcttc cagcggccga cgcaggggac
     1441 ggtccggcac gacgggcagc ccatcaccgg ctggcgaccg caccggatcg cggcgcgggg
     1501 gctcgtgcgc acgttccaga agacgagcgt gttccccggc ctgagcgtgc gggagaacgt
     1561 gctgaccggg ctacacctcc gcgggcgagt gggcgtggcc gcggccctgc tgacccgcgg
     1621 ccgagtgcgc gcggaggagc gtcggctcga ggcggaggcc gagcgggtga tcgacttcgt
     1681 cgggctcggg tcgcgccggg gcgaggcggc gaatggcctc tcctacggcg accagcggct
     1741 cctcgagctg gcggtggccc tggccgcgca gccgcgcctg ctcatgctgg acgagcccgc
     1801 ctcgggcatg agcgcctccg agaaggtcac ggtcgcggcg ctgatcggcc gaatccgcga
     1861 gcagggcatc acggtgttcc tggtggagca cgacatgcgg ctggtcatgg gcatctcgga
     1921 tcgcgtgctg gtgctcaact acggccggct gatcgccgac ggcgacccgg ccgcggtgca
     1981 gcgcgacccc gaggtcgtcc gcgcctacct gggatcggga gcccatgctc acgcttgagc
     2041 gggtggccgc gggctacggg cgcatcaccg cgctccacgg cgtgtcgctc acggtgggcg
     2101 cggccgagtt cgtgtgtctc atcggggcca acggggccgg caagtccacc acgctcaaga
     2161 cgatctccgg actggtgcgg gcgggggccg ggcggatcgt cttcgacggg caggagatcc
     2221 agcgcctggc gccccaggac atcctccgcc gcggcatcgc ccactgcccg gagggccggc
     2281 gcgtgttccc gcacatgacg gtggaggaga acctccagat gggcgcctac gtgcgcaccg
     2341 acggggtggc ggtcgcggcg gaccgcgagc gggtcttcgg ccacttcccg atcctggccg
     2401 accggcggcg ccaggccgcg ggcacgctct cgggcggcga gcagcagatg ctggccatcg
     2461 gccgctgcct gatggcgcgc ccccggctca tcctgttcga cgagccctcg ctcgggctgg
     2521 ctcccaccgt gaccgagacc gtgttccaga tcatcgcggg catccagcgg gaaggcacca
     2581 cggtgctgat ggtcgagcag aacgcctaca tggcgctcca gctcgccggc cgcgcctacg
     2641 tcatggagac cggccgcatc accctcgaag gccccgccaa ggacctgatg cgaaacgacc
     2701 acgtcaagaa agcctatctg gggggctaag aggctaagca atggccgcgc cggaacggat
     2761 gtccgccgcc tgggggagcg cgatcgctgc gccggaacgg atgtccgccg cctgggggcg
     2821 tgcgggggcc atttctgggc ccccgcagat gaagagcttc aaaggcagta agcggatgtc
     2881 cgccgcctgg gggcgtgcgg gggccatttc ttggcccccg cagatgaaag gcttcaaaga
     2941 actaaacggt tgtccgcaga agccccaagg agctccccga taacatgggc gccaagtatt
     3001 tcggggcctc ggtgaaacgc aaagaagacc cgcgctacct gcgcggcgag ggccgcttcg
     3061 tggacgacat caagctgccc ggcatgctcc atgccgcctt cgtgcggagc ccctacgcgc
     3121 acgcgcgcat caccgcgatc cgcaccgagg ccgcgaagcg cctgcccggc gtcgcccacg
     3181 tgttcacgtt cgccgacctc gagcgctgga tgaagccgct gccgctcttc ggcgcgatcc
     3241 cgcccgggct ctccgcgcgg gtggcggtga tgatgcggca gatcggtcag ctcgccctgt
     3301 gccgcgacga ggcccgccac gtgggcgaga tcgtggccat ggtgctcgcg agcagccgcg
     3361 ccctggccga ggacggctgc gagctggtcg aggtcgacta cgagcccctg ccggtgctgg
     3421 ccgacgcggt ggccgccgcc ga
//
LOCUS       DATMCV010000870         3859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_710414, whole genome shotgun sequence.
ACCESSION   DATMCV010000870 DATMCV010000000
VERSION     DATMCV010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710414"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VNU03_19960"
     CDS             <1..1064
                     /locus_tag="VNU03_19960"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP76805.1"
                     /translation="SGDYPRCMALALEALDLSAARREQAEARAQGRYLGIGLVNYVEA
                     TATVPNESAVVRVHADGRVTVVSGAAPQGQGHVTMFSRLVGRELGVDADAIEVLTGDT
                     ALVETGGGTYGSRTAAIGGSAALLASRAVLDKARRVAAHLLEAAPADVVVEAGRFAVR
                     GLPSRTLGWSDVAAAAHGGHVPGEAAGLEDTHVFAVSQSSFANGSHAVVLEVDPETGA
                     VTVLRWIVAHDCGHVLEPGIVDGQIHGAVVQGLPDALNVQLAYDEGGQRLTATLMDYV
                     LATAADAPATFELRHLESPTPLNPLGAKGAGEGGIMPVHPLIAQAIEDALAPFGVGPV
                     ERVPVTREDIHGMVRAARR"
     gene            complement(1155..1991)
                     /locus_tag="VNU03_19965"
     CDS             complement(1155..1991)
                     /locus_tag="VNU03_19965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWP76806.1"
                     /translation="MALGSALCAACATLLIQRGLQRSNFYAGAWINVVVGAVAAWSAT
                     LLIVPWHAYTWRAVPYFVFSGVVGTAGGRLFRVLAIQKVGAPVAAAVNNLAPLVATGL
                     AILLLGEHVTLPILGGTLVIVAGTILLSLSGRYVGFRVRHLAYPFIAASCFGTVAVVR
                     KLGLSTAGPLFDAAINVSAALVASTTFVAASGHLASLRCDRKSLGWFVAGGIAENSGV
                     FLILLALGFGDVSVVIPLAGTAPLFVLLITYFMPREAARLHWRLVAGAVMIVIGVMVL
                     TG"
     gene            complement(2019..2933)
                     /locus_tag="VNU03_19970"
     CDS             complement(2019..2933)
                     /locus_tag="VNU03_19970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP76807.1"
                     /translation="MKLSVEFPSVSYREGPAAVAALARAIERIGYDHIDIFDHVVMGV
                     PIEGRERGPYNPQMPILEALMGLAYMAAVTTRVTLGTEVLVLPQRQPTLVAKQVSTLD
                     TLSGGRARLGVGVGWQESEYEALGERFHDRGARMDEAIRLLRAYWADAEVNMASAHYP
                     TVSMAMEPKPPQGGRLPIWIGGLSEAAFRRVGRLGDGWLASRVTDAAGARQAIETIHR
                     HAEAAGRDPGAIGLQSMVAPPPRDAAGKTFYGDHDQVVARVASLKAMGFGWVALNATA
                     IFQAGARSVDAMTQQLHRLHDRIRAEVG"
     gene            3026..>3859
                     /locus_tag="VNU03_19975"
     CDS             3026..>3859
                     /locus_tag="VNU03_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HWP76808.1"
                     /translation="MGGLGFLTMLRSRAAADLVEPAAARPTQRLLSLDVFRGLTIAAM
                     LLVNNPGTWSHVYPPLEHAEWNGWTPTDLVFPFFLFIVGVATALSLGGLRDAGTGRAN
                     VFRKAFVRAAIIFGLGLLLQGFPHYDLPHLRIPGVLQRIAIAYLLTAIVVLLTGVRGQ
                     VLTLLGLLLGYWTLMTLVPVPGVGRGVLEPEKNLSNWLDFRLLGVNHVWHETRTWDPE
                     GLLSTLGAVASVLCGVLAGHWIRSRHRTDAKAFGLFYAGGMILLLGWVWSHIFPINKS
                     LW"
BASE COUNT          533 a         1392 c         1419 g          515 t
ORIGIN      
        1 acagcggcga ctacccgcgc tgcatggccc tggcgctcga ggcgctggac ctgtccgcgg
       61 cccgccggga gcaggcggag gcgcgcgcgc agggccgcta cctcgggatc ggcctcgtca
      121 actacgtgga ggccaccgcc accgtgccga acgagagcgc ggtggtgcgc gtgcacgccg
      181 acggccgcgt caccgtggtg agcggggccg cgccgcaggg gcagggccac gtgactatgt
      241 tctcgcgcct ggtcggtcgg gagctcggcg tcgacgccga cgcgatcgag gtgctgaccg
      301 gcgacaccgc gctggtcgag accggcggcg gcacctacgg cagccgcacc gcggccatcg
      361 gcggcagcgc ggcgctgctg gcctcgcgcg cggtgctcga caaggcgcgc cgggtggccg
      421 cgcacctgct cgaggccgcc cccgccgacg tggtggtcga ggccggccgc ttcgcggtgc
      481 gcgggctgcc gagccgcacg ctcggctggt ccgacgtcgc ggcggcggct cacggaggcc
      541 acgtgcccgg cgaggcggcc gggctcgagg acacgcacgt cttcgcggtc tcgcagtcga
      601 gcttcgccaa cggcagccac gcggtggtac tcgaggtgga cccggagacc ggtgcggtga
      661 ccgtgctccg ctggatcgtg gcccacgact gcggccacgt gctcgagccg ggcatcgtgg
      721 acggccagat tcacggcgcg gtggtgcagg gcctgcccga cgcgctcaac gtgcagctgg
      781 cctacgacga gggcgggcag cgcctcaccg cgacgctgat ggactacgtg ctggccaccg
      841 cggcggatgc ccccgcgacc ttcgagctgc gccacctgga gtcgcccacg ccgctgaacc
      901 cgctcggagc caagggcgcg ggcgagggcg ggatcatgcc ggtgcacccg ctgatcgcgc
      961 aggccatcga ggacgcgctg gcgccgttcg gcgttggccc ggtcgagcgc gtgccggtga
     1021 cgcgggagga catccacggc atggtgcggg cggcgcggcg gtagctctcc cctcgccccc
     1081 tcgccccctc accaccgggc cccctcaccc tgccctctcc ccctcggcgg gggagaggga
     1141 gagatagggg agagctagcc ggtcaggacc atcacgccga tgacgatcat caccgcgccc
     1201 gcgacgaggc gccagtggag gcgggctgcc tcccggggca tgaagtaggt gatgagcagg
     1261 acgaagagcg gcgcggtgcc cgcgagcggg atgaccacgc tgacgtcgcc gaacccgagc
     1321 gcgagcagga tcaggaacac gccgctgttc tccgcgatgc cgcccgcgac gaaccagccg
     1381 aggctcttgc ggtcgcagcg gagcgaggcg aggtggccgg aggcggccac gaaggtggtg
     1441 gacgcgacca gcgccgcgga gacgttgatc gccgcgtcga agagcgggcc cgcggtggag
     1501 agcccgagct tgcgcaccac cgcgacggtg ccgaagcacg acgccgcgat gaacggatag
     1561 gcgaggtggc ggacgcggaa gcccacgtag cggccgctca gcgagagcag gatcgttccc
     1621 gccacgatga ccagggtgcc gcccaggatc ggcagcgtga cgtgctcgcc cagcagcagg
     1681 atggcgaggc cggtggccac gagcggggcc aggttgttga ccgcggccgc caccggcgcg
     1741 cccaccttct ggatggcgag cacccggaag agccggcccc ccgcggtgcc gaccaccccg
     1801 gagaacacaa agtaggggac cgcccgccag gtgtaggcgt gccagggcac gatgaggagc
     1861 gtggccgacc acgcggcgac cgcgcccacc accacgttga tccaggcgcc cgcgtagaag
     1921 ttggagcgct gcagcccgcg ctggatgagg agggtggcgc acgcggcgca gagcgcgctc
     1981 ccgagcgcca tcaactgcgg aagcggagtc aatgcggctc agccgacctc ggcgcggatg
     2041 cgatcgtgca gccggtggag ctgttgcgtc atcgcgtcga cggagcgcgc gccggcctgg
     2101 aagatcgcgg tggcgttgag cgcgacccag ccgaagccca tcgccttgag cgaggccacc
     2161 cgggccacca cctggtcgtg gtccccgtag aacgtcttgc ccgccgcgtc gcggggcggg
     2221 ggagccacca tgctctggag cccgatggct cccggatcgc ggccggccgc ctccgcgtgg
     2281 cgatggatgg tctcgatggc ctgccgcgcg ccggccgcgt cggtgacgcg gctcgcgagc
     2341 cagccgtcgc cgagccggcc cacgcggcgg aacgcggcct ccgagaggcc gccgatccag
     2401 atcggcaggc ggccgccctg cggcggcttc ggctccatcg ccatcgacac cgtcgggtag
     2461 tgcgcgctcg ccatgttgac ctcggcgtcg gcccagtagg cgcgcagcag ccggatcgcc
     2521 tcgtccatcc gcgccccgcg gtcatggaac ctctcgccga gcgcctcgta ctccgattcc
     2581 tgccagccca ctcccactcc gagccgcgcc cggccgcccg acagcgtgtc gagcgtgctc
     2641 acctgcttgg ccaccagggt gggctggcgc tgcggcagga ccagcacctc ggtgccgagc
     2701 gtcacccgcg tggtgaccgc ggccatgtag gcgagcccca tcagggcctc gaggatcggc
     2761 atctgggggt tgtaggggcc gcgctcgcgc ccctcgatcg gaacgcccat caccacgtga
     2821 tcgaagatgt cgatgtggtc gtagccgatg cgctcgatcg cgcgggccag cgcggcgacc
     2881 gcggccgggc cctcgcggta ggacacgctc gggaactcga cggacagctt catgcggacg
     2941 ccctcatgac ggcctccttt ggccaggcgc ggctacgata tcacggagcg ctcggaggct
     3001 tcccgccggt gctacgatgc gggacatggg cgggctaggg ttcctgacga tgctccggtc
     3061 gcgggcggcg gccgatctcg tcgagccggc ggccgcccga ccgacgcagc ggctgctctc
     3121 cctcgacgtc ttccgcgggc tcaccatcgc ggccatgctg ctggtcaaca accccggcac
     3181 ctggagccac gtctacccgc cgctcgagca cgcggagtgg aacgggtgga cgccgaccga
     3241 cctggtgttc ccgttcttcc tcttcatcgt cggcgtcgcg accgcgctct cgctcggcgg
     3301 gctccgggac gcgggcaccg gacgggccaa tgtcttccgc aaggcgttcg tgcgcgccgc
     3361 gatcatcttc gggctggggt tgctgctcca ggggtttccc cactacgacc tcccccacct
     3421 ccgcatcccc ggcgtgctgc agcggatcgc gatcgcgtac ctgctcaccg cgatcgtcgt
     3481 cctgctcacc ggggtgcgag gtcaggtcct caccctgctc gggctgctgc tcgggtactg
     3541 gacgctcatg acgctggtgc ccgtccccgg ggtcgggcgc ggcgtgctcg agccggagaa
     3601 gaacctctcg aactggctcg acttccgcct gctcggggtc aaccacgtct ggcacgagac
     3661 gcgaacctgg gacccggagg ggttgctgag cacgctgggc gcggtcgcct ccgtgctctg
     3721 cggggtgctg gccggccact ggatccgctc ccgccaccgg acggacgcga aggccttcgg
     3781 gctcttctac gcgggcggca tgattctcct gctcgggtgg gtctggagcc acatcttccc
     3841 gatcaacaag agcctgtgg
//
LOCUS       DATMCV010000871         4915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_843416, whole genome shotgun sequence.
ACCESSION   DATMCV010000871 DATMCV010000000
VERSION     DATMCV010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843416"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VNU03_19980"
     CDS             complement(<1..1217)
                     /locus_tag="VNU03_19980"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76809.1"
                     /translation="MDMLVRGRHVITDPAVGDPSVLEDGAVLVSGSRIAAVGAWAALR
                     RRHPRARVMGNGKQLLMPGLVDAHSHGRALSPIQKGVLNDYLENNLLDWAFMPVFDPE
                     LMAALAAWRHLRSGCTTIHHMGFDTEGPQARGRSEKAIRTYLRSGIRLAFAPGVRNVD
                     KLVLDSVTFLETLPPDLQAFADPLVNVDAKRMEEEYFALFAHLHGRFASEDTRVLLSP
                     SWAQACTESFLLRAKATADRLGKVPIHMHCVQTPIQRAFSFRKYGKSAIAWLDDLGLV
                     DENLALGHAIWVSEDDIDRLATRRASITSHPSCNLGMRNGLAPIYAMHRRGVNVAMGL
                     DDKTINDDEDAVMELRMLHKLHRVPDYDLRTPALTAYDVLRMGTLNGARAIGFGGQIG
                     ALVPGMKADMILV"
     gene            1379..2245
                     /locus_tag="VNU03_19985"
     CDS             1379..2245
                     /locus_tag="VNU03_19985"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWP76810.1"
                     /translation="MTVRVALAQFSGHIDKNINIEKAESLARQAAGNGAKVVCFPELC
                     TTIYFCFDRDKKWFETAEPVPGPAVDRLRRVAKETGTVIVYPLYENDGGVLYNTAAVI
                     GPDGELIGKYRKMSIPQILRTVKAGETPGDERFFFQPGNLGFPVFDTPFGLKIGILIC
                     YDRHFPEAARTLGLNGAHLLLVPTATYREWIRDVWEVELRGHAIANMFYVGGVNKVGK
                     DVGGPPDRHYFGSALFIDPKGGVMARAGDKEDEILYADIDPKTCDDVRDLWGFLKFRR
                     PDAYGDVVQGTR"
     gene            2250..3611
                     /locus_tag="VNU03_19990"
     CDS             2250..3611
                     /locus_tag="VNU03_19990"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76811.1"
                     /translation="MDADLFVRDCRVVKPDGVVDCGLAIRGGSIAAVLRPGETVTARR
                     TIDADGRFVVPGLIDTHLHLGNAAQAFASDCATESRHAVTGGVTTLMPFVITRGSYLD
                     ALDEMRRAVAEQSAVDMLFHAIIVREAQIDEIPRMASEFGVRSFKAFMAYKGREISPS
                     GIQGMGDDQIFSMFQRVKDVPGGVAIVHCENMEIIELHQKPLMAAGRQDTAAWSDGRP
                     AWGELEAIRRMCQFAEAAAVQLLIPHMGVGLGSEFLRQKAWGPGRVATESCPHYLAFD
                     KDTDRGVQGKVNPPLRGRDHIDALWQRLTDGTVDVMGSDHCPYTKAVKGDELWAARAG
                     ITAGSAMILPVLLTEGVNRGRLTMPDVVRLTSYNAARLFGLYPKKGALEAGSDADLVI
                     VDTDREVKVDLPALHSAVDFSPYEGYVARGWAATTIAGGRIAYDAGEVVSDGARGRYL
                     ARS"
     gene            3633..4637
                     /locus_tag="VNU03_19995"
     CDS             3633..4637
                     /locus_tag="VNU03_19995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HWP76812.1"
                     /translation="MLKFGVMLRPGHFPFSHMLTTMRVAEDAGFEFMWFGDSHLIWQE
                     VSPYLAAAASGSRRLTVGPLVSNPVTRHPTVMASMIATIAQLYEGRAVLGVGRGDSAV
                     RTLGLPPMPVKQFRETLRTIRALCRGDEVDYGGTAIHFPWLTHKVPVWVAAYGPRVLE
                     LAGAEGDGVILQLASPSVVEWSVGHVRRGREQAGLPWAGFEVLAGAPTYVSDDRAHAL
                     SRVRGFPATVSNHVRDLLRHHAPSELPAELVEGMEQVAGYDYQQHGQPDAPHVRAVTD
                     AMAERLTLVGTAAEVLDKIKKLEAAGVTQVCLYLSIVEPERHFSTLETYGREIIPAFR
                     "
     gene            4679..>4915
                     /locus_tag="VNU03_20000"
     CDS             4679..>4915
                     /locus_tag="VNU03_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76813.1"
                     /translation="MTLRLLALLAVAVISAVLPTTAPAQENVLNVGKYGQLASYDPHA
                     GGLDSMWWTLNNFYESLVDLNDDINGLRPVLATAW"
BASE COUNT          736 a         1758 c         1671 g          750 t
ORIGIN      
        1 tcgacgagga tcatgtccgc cttcatcccg ggcacgagcg cgccgatctg cccgccgaaa
       61 ccaatggcgc gggctccgtt cagagttccc atgcgcagca cgtcgtaggc ggtgagggcc
      121 ggggtgcgca ggtcgtagtc gggcacgcgg tggagcttgt gcagcatgcg cagctccatc
      181 accgcgtcct cgtcgtcgtt gatggtcttg tcgtcgagcc ccatcgccac gttgacgcct
      241 cgccggtgca tggcgtagat cggcgccagg ccgttgcgca tgccgaggtt gcagctcggg
      301 tggctcgtga tggaggcccg gcgggtcgcg agccggtcga tgtcgtcctc gctgacccag
      361 atggcgtggc ccagggcgag gttctcgtcc acgaggccca ggtcgtcgag ccaggcgatg
      421 gcgctcttgc cgtactttcg gaaagaaaac gcccgctgga tcggcgtctg cacgcagtgc
      481 atgtggatgg gcaccttgcc gagccggtcc gccgtcgcct tggcgcggag gaggaagctc
      541 tcggtgcacg cctgcgccca gctcggcgag agcagcacgc gcgtgtcctc ggacgcgaag
      601 cgcccgtgca ggtgcgcgaa cagcgcgaag tactcctcct ccatccgctt ggcgtcgacg
      661 ttgaccaggg ggtcggcgaa ggcctggagg tccggcggca gcgtctcgag gaaggtcacg
      721 gagtccagca ccagcttgtc cacgttccgc accccgggcg cgaaggcgag ccggatcccg
      781 ctgcgcaggt aggtgcggat ggccttctcc gaccggccgc gcgcctgcgg gccctccgtg
      841 tcgaacccca tgtggtggat cgtcgtgcac ccgctccgca gatgtcgcca ggcggccagg
      901 gcggccatca gctccgggtc gaagacgggc atgaaggccc agtcgaggag gttgttctcc
      961 aggtagtcgt tgagcacgcc cttctggatc gggctcagcg cccgcccgtg actgtgggcg
     1021 tcgaccagcc ccggcatcag cagctgcttg ccgttgccca tcacccgcgc gcggggatgc
     1081 cgccggcgga gcgcggccca ggccccgacc gcggcgatcc gggagcccga gaccagcacc
     1141 gccccgtcct cgagcacgct cgggtccccg acggcggggt ccgtgatcac gtgccgtcca
     1201 cgcaccagca tgtccatgcg cgcacctccg tgtggtggct cccggccgcg cggccggctt
     1261 cgaccgggct atcctactcg tcagctggtt ccagaagaag ccctgtggcc ccttgcgcaa
     1321 tgtggcccgc ggcggtacaa aagccgggcc tggccatccc agaggaggat ccgatccgat
     1381 gaccgtacgg gttgcgctcg cccagttcag tggacacatc gacaagaaca tcaatataga
     1441 gaaagccgag agccttgccc ggcaggccgc cggaaacggg gccaaggtgg tgtgcttccc
     1501 ggagctctgc acgaccatct acttctgctt cgaccgcgac aagaagtggt tcgagaccgc
     1561 cgagcccgtc cccggccccg ccgtcgaccg tctgcgccgg gtcgccaagg agaccgggac
     1621 cgtcatcgtc tacccgctct acgagaacga cggcggcgtg ctctacaaca cggccgcggt
     1681 gatcggtccc gatggcgagc tcatcggcaa gtaccgcaag atgtccatcc cgcagattct
     1741 ccgcaccgtg aaggcgggag agacgcccgg tgacgagcgc ttcttctttc agcccggcaa
     1801 cctcggcttc ccggtcttcg atacgccgtt cggcctcaag atcggcatac tcatctgcta
     1861 cgaccgccac ttcccggagg ccgcccggac gctcggcctg aacggagcgc acctgctcct
     1921 ggtgccgacc gcgacctatc gcgagtggat ccgcgacgtc tgggaggtcg agctgcgcgg
     1981 ccacgccatt gccaacatgt tctacgtggg cggcgtcaac aaggtgggca aggatgtcgg
     2041 cggccctccc gatcgtcact acttcggctc ggcgctcttc atcgatccga aaggcggggt
     2101 gatggcccgc gccggcgaca aggaggacga gatcctctac gccgatatcg accccaagac
     2161 atgcgacgac gtgcgcgacc tctggggctt cctgaagttc cgtcgtccgg acgcctacgg
     2221 ggacgtggta cagggcacgc gctgagccga tggacgccga cctgttcgtc cgcgactgcc
     2281 gcgtcgtcaa gcccgacggc gtcgtcgact gcgggctcgc catccggggc gggtcgatcg
     2341 cggccgtgct ccgccccggg gagaccgtca ccgcccgccg caccatcgac gcggatgggc
     2401 gcttcgtcgt ccccgggctc atcgatacgc acctgcacct cggcaacgcg gcccaggcct
     2461 tcgcctcgga ctgcgcgacg gagagccggc acgccgtcac cggcggcgtg accacgctga
     2521 tgcccttcgt gatcacccgg ggctcctatc tggacgctct ggacgagatg cggcgggcgg
     2581 tggcggagca gagcgcggtc gacatgctct ttcacgccat catcgtccgc gaggcccaga
     2641 tcgacgagat cccgcgcatg gcctcggagt tcggcgtgcg ctccttcaag gcgttcatgg
     2701 cctacaaggg gcgcgaaatc tcgccgagcg ggatccaggg catgggcgac gaccagatct
     2761 tctcgatgtt ccagcgcgtg aaggacgtgc ccggcggcgt ggccatcgtg cactgcgaga
     2821 acatggagat catcgagctg caccagaagc cgctcatggc cgccgggcgc caggacaccg
     2881 cggcatggtc cgacggccgg ccggcgtggg gcgagctcga ggcgatccgg cgcatgtgcc
     2941 agttcgccga ggccgccgcc gtccagctgt tgatccccca catgggcgtg ggcctcggct
     3001 ccgagttcct gcgccagaag gcgtgggggc ccggccgcgt cgccacggag agctgccccc
     3061 actacctggc cttcgacaag gacaccgacc gcggcgtgca gggcaaggtg aacccgccgc
     3121 tccgcgggcg cgaccacatc gacgcgctct ggcagcgcct caccgacggc accgtcgacg
     3181 tgatggggtc cgatcactgc ccttacacga aggcggtgaa gggcgacgag ctctgggccg
     3241 cgcgcgccgg gatcaccgcg ggaagcgcca tgatcctccc ggtgctgctg accgaggggg
     3301 tcaaccgcgg gcgcctcacc atgccggacg tcgtgcgcct cacctcgtac aacgcggcgc
     3361 ggctcttcgg gctctatccg aagaagggcg cgctcgaggc ggggtcggac gccgacctcg
     3421 tcatcgtcga caccgatcgc gaggtcaagg tggatctccc cgcgctccac tccgcggtcg
     3481 acttcagccc gtacgaggga tacgtggcgc ggggctgggc ggccaccacc atcgccggtg
     3541 gccgcatcgc ctacgatgcg ggagaggtcg tctccgacgg cgcccgcggg cgctacctcg
     3601 cccgaagcta gcgacccgga gcccattcgg ccatgctcaa gttcggcgtc atgctgcgcc
     3661 ccggccactt ccccttctcg cacatgctca ccaccatgcg ggtggcggag gacgcggggt
     3721 tcgagttcat gtggttcggc gactcgcacc tcatctggca ggaggtcagc ccctacctgg
     3781 ccgccgccgc ctcgggcagc cgccgcctga ccgtcggccc gctcgtctcc aacccggtga
     3841 cccgccaccc gaccgtgatg gcgagcatga tcgcgaccat cgcccagctc tacgagggcc
     3901 gcgccgtcct gggcgtgggc cgcggcgaca gcgcggtgcg caccctcggg ctaccgccga
     3961 tgccggtcaa gcagttccgc gagaccctgc ggacgatccg cgcgctctgc cgcggcgacg
     4021 aggtggacta cgggggcacc gcgatccact ttccgtggct cacccacaag gtcccggtgt
     4081 gggtggccgc ctacggcccc cgggtgctcg agctggccgg ggccgaaggg gacggcgtca
     4141 tcctccagct cgcctcgccc agcgtggtgg agtggtcggt gggccacgtc cggcgcggac
     4201 gcgagcaggc cggcctgccc tgggcgggct tcgaggtgct cgcgggcgct ccgacgtacg
     4261 tctccgacga tcgcgcgcac gccctctcgc gggtgcgcgg gtttcccgcc acggtgtcga
     4321 accacgtccg ggacctgctg cgccaccacg cgccctcgga gctgcccgcc gagctcgtgg
     4381 agggcatgga gcaggtcgcc gggtacgact atcagcagca cgggcagccc gatgcgcccc
     4441 acgtccgcgc ggtcaccgac gcgatggccg agcggctcac cctcgtcggg acggccgccg
     4501 aggtgctcga caagatcaag aagctcgaag ccgcgggcgt gacgcaggtg tgcctctacc
     4561 tgtcgatcgt ggagcccgag cggcacttct cgacgctgga gacctacggc cgcgagatca
     4621 ttccggcgtt ccgctgatgg ccgccgcttc cgccaccccg cacccaggag gagctgtcat
     4681 gactctccgg ctgctcgcgc tgctcgccgt cgccgtcatc tccgccgtcc tcccgaccac
     4741 ggcgccggcc caagaaaacg tgctcaacgt cggcaagtac ggccagctcg ccagctacga
     4801 ccctcacgcg ggcgggctcg acagcatgtg gtggaccctc aacaacttct acgagtcgct
     4861 cgtcgacctg aacgacgata tcaacggcct ccggccggtg ctggcgacgg cgtgg
//
LOCUS       DATMCV010000872         4082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_180457, whole genome shotgun sequence.
ACCESSION   DATMCV010000872 DATMCV010000000
VERSION     DATMCV010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180457"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VNU03_20005"
     CDS             complement(<1..451)
                     /locus_tag="VNU03_20005"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP76814.1"
                     /translation="MAIDPVSLGIMWDRLISITDEVLSALVRTSFSSNVRDSYDLSVL
                     LFSADGRSLVQGTYSVPSFTGTGPATLRHMLDRFPADTLRPGDVIATNDPWMGTGHLF
                     DVNVMAPIFLRDRLIGYSMSITHLPDIGGRGFSATGRTIYEEGLRLPV"
     gene            complement(451..2550)
                     /locus_tag="VNU03_20010"
     CDS             complement(451..2550)
                     /locus_tag="VNU03_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP76815.1"
                     /translation="MPSYRIGIDIGGTFTDFVLVREDSGAMRFAKALTTPRDPSECVL
                     AGVRDLLAEHRVPIDQVQAIVHGTTLATNAVIERRGATTGLLTTKGFGDLLDLGRETR
                     YDLFDLRAVYPAPLVPRGLRRELTERLRYDGEVLEPLDVAEVERVLDELVHGHHVEAL
                     AICLLHAYANPVHEQRVQALARARFPRLAVSTSSEVFPFMGEYERFTTTTMNAYVQPM
                     IDRYLDRLERELAGQGFRGRLYVMTSAGGTMTLEAARRFPVRLLESGPVAGALMSASL
                     GRQLGAPDLLSFDMGGTTAKGCIVLDHEPRKRYALEVARVHEFKPGSGLPVRGPFIDM
                     VEIGAGGGSIAEVDARGLIRVGPRSAGADPGPACYGRGGTRATLTDANVLLGYLDPAF
                     FNGGRMTLDVEAAREAVTTHVARPLGLTPPAAAWGIHEIINEDVARAFRVHAAERGVD
                     YRRCGMVAFGGSGPIHALRVARKLRIPRVIFPLGAGVMSAVGLLVSPLSFDAARSHRV
                     RLDQLEAKAFAQLLQALVDEAIARLEADGIPADRIAVERRLDMRHEGQGFEIEVPVPV
                     AEDPQHALDALPALFKARYAELFAVTSNDQPIEVVNWKVAVTGPRPANADAYRLEQER
                     AVRASLKGARPAFFGAADPVECPVYDRYALAPGARLTGPALLEERESTCVVGPGDRIE
                     VDGAGNLIAEISYGGDG"
     gene            complement(2588..3403)
                     /locus_tag="VNU03_20015"
     CDS             complement(2588..3403)
                     /locus_tag="VNU03_20015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76816.1"
                     /translation="MRGATVPQMVSGAVLLGFLALTAFPEALAPWDPLKTQPGQRLRP
                     PSAAHPLGTDYLGRDVLSRVIHGARVSLVPAIAVVLAAGLAGSAVGLIAGYLGGWIDE
                     TTMRLADVFLAVPALILAMAIATALGPSARNAALAVIVVWWPSYARLARGEAQVLRAV
                     EFIQAAQASGASQSRILIMHMLPNQVSSLVVKASLDVGTVLLLLTGLSFLGIGAQPPS
                     PEWGLEVATSRQYMFERPWYTIGASAAIFLAVYSMNLFGDLVRERLDPRLRST"
     gene            complement(3403..>4082)
                     /locus_tag="VNU03_20020"
     CDS             complement(3403..>4082)
                     /locus_tag="VNU03_20020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76817.1"
                     /translation="LVGGALGVLAAVRRGSAIDTVATAGPVAQLSVPVFVMGLVLLLV
                     FYRWLGWLPYGGRLDSMVPAPPGITGLYLIDSLLARDGRAFRDAAAHLVLPALALSNL
                     TLAEMTRITRSAVLDVLGADHVRTARSKGLAERAVLWRHVVKNAAIPVVTVMGLRVGF
                     LMGGAVITETIFAWPGIGRYAWEGARNVDLNVVMGVTLVVALVYSLVNLAVDVLYSVL
                     DPRVRLS"
BASE COUNT          631 a         1392 c         1554 g          505 t
ORIGIN      
        1 ccaccggaag gcgcagcccc tcctcgtaga tggtgcggcc ggtggccgag aagccgcgcc
       61 cgccgatgtc gggcaggtgg gtgatggaca tcgaatagcc aatcaggcgg tcgcggagaa
      121 agatcggcgc catgacgttg acgtcgaaca ggtggccggt gcccatccat ggatcgttgg
      181 tggcgatcac gtcgcccggc cggagcgtgt cggcggggaa gcgatccagc atgtgacgca
      241 gggtggcggg cccggtgccg gtgaaggaag gcacgctgta cgtgccctgg acgagcgagc
      301 ggccgtcggc gctgaacagg agcaccgata gatcgtagct gtcgcgcacg ttcgacgaga
      361 acgacgtccg caccagcgcg gacagcacct cgtcggtgat cgagatcagc cgatcccaca
      421 tgatgccgag gctgaccggg tcgatggcca tcagccgtct cctccgtagg aaatctccgc
      481 gatcaggttg cccgcgccgt cgacctcgat ccggtcgccc gggccgacca cgcaggtcga
      541 ctcccgctcc tcgagcaggg cgggtccggt gagccgcgcg ccgggcgcca gcgcgtagcg
      601 atcgtagacc gggcactcga ccgggtcggc cgcgccgaag aacgccggcc gcgcgccctt
      661 gagcgaggcg cggaccgcgc gctcctgctc gaggcggtac gcgtccgcgt tggcggggcg
      721 cggtccggtg accgcgacct tccagttgac gacctcgatg ggttgatcgt tcgaggtcac
      781 cgcgaacagc tccgcgtagc gcgccttgaa cagcgcgggc agggcatcga gcgcgtgctg
      841 cggatcctcg gccacgggca ccgggacctc gatctcgaag ccctggccct cgtggcgcat
      901 gtcgaggcgg cgctccaccg cgatccggtc ggccgggatt ccgtcggcct cgagccgggc
      961 gattgcctcg tcgaccagcg cctgcagcag ctgggcgaac gccttggcct cgagctggtc
     1021 caggcgcacg cggtgcgagc gggcggcgtc gaaggacagc ggcgagacca gcagccccac
     1081 cgcggacatc acgccggccc ccagggggaa gatcacgcgc gggatgcgca gcttgcgcgc
     1141 gacgcgcagg gcgtggatcg ggccggagcc gccgaaggcc accatgccgc agcgccggta
     1201 gtccacaccg cgctccgcgg cgtggacgcg gaaggcacgc gccacgtcct cgttgatgat
     1261 ctcgtggatg ccccacgccg cggccggcgg cgtgaggccg agggggcgtg ccacgtgcgt
     1321 ggtcaccgcc tcgcgcgccg cctcgacgtc cagggtcatc cggccgccgt tgaagaaggc
     1381 cggatcgagg tagccgagca gcacgttggc atcggtgagc gtcgcccgcg tgccgccgcg
     1441 cccgtagcag gccgggcccg ggtcggcgcc ggcgctgcgc gggccgacgc ggatgaggcc
     1501 gcgcgcgtcg acctcggcga tgctcccgcc gcccgcgccg atctcgacca tgtcgatgaa
     1561 cggaccccgc accggcagcc cgctgcccgg cttgaactcg tgcacgcgcg ccacctcgag
     1621 cgcgtagcgc ttgcggggct cgtggtccag cacgatgcag cccttcgcgg tggtgccgcc
     1681 catgtcgaac gagaggaggt cgggcgcgcc gagctggcgg ccgaggctgg ccgacatcag
     1741 ggcgcccgcg acggggccgg actcgaggag gcgcaccggg aagcgccgcg ccgcctcgag
     1801 ggtcatcgtg ccgcccgccg aggtcatcac gtagaggcgg ccgcggaagc cctggccggc
     1861 cagctcgcgc tcgaggcggt ccaggtaacg gtcgatcatc ggctgcacgt aggcgttcat
     1921 ggtggtggtg gtgaagcgct cgtactcgcc catgaacggg aagacctcgg acgaggtcga
     1981 gacggcgagg cgcgggaatc gggcgcgcgc cagcgcctgc acccgctgct cgtggaccgg
     2041 gttcgcgtag gcgtggagga ggcagatcgc gagcgcctcg acgtggtggc cgtggaccag
     2101 ctcgtcgagg acgcgctcga cctccgcgac gtcgagcggc tcgagcacct cgccgtcgta
     2161 gcggagccgc tcggtcagct cgcgccggag gccgcggggc acgagcggcg ccgggtatac
     2221 cgcgcgcagg tcgaagaggt cgtagcgggt ctcgcgcccg aggtcgagaa ggtcgccgaa
     2281 gcccttcgtg gtgaggagcc cggtggtggc gccgcggcgc tcgatcaccg cgttggtagc
     2341 cagcgtcgtc ccgtgcacga tggcctgcac ctgatcgatg gggacgcggt gctcggccag
     2401 cagatcgcgg acgccggcca gcacgcactc ggagggatca cggggggtgg tgagcgcttt
     2461 cgcgaagcgc atggccccgc tgtcctctcg gacgaggacg aagtcggtga aggtgccgcc
     2521 gatgtcgatg cctatgcgat agctgggcat gcttgctcct agatagcgtc ggcgcccgga
     2581 gggcgcacta ggtgctccgg aggcgcgggt cgagacgctc ccggaccagg tcgccgaaga
     2641 ggttcatgct gtacaccgcg agaaagatgg ccgcgctcgc gccgatcgtg taccacggcc
     2701 gctcgaacat gtactggcgc gacgtggcga cctcgagccc ccactcgggg gacggcggct
     2761 gggcgccgat gccgaggaag ctgaggccgg tcagcagcag cagcacggtg ccgacgtcaa
     2821 ggctcgcctt gaccacgagc gacgacacct ggttcggcag catgtgcatg atcaggatgc
     2881 gcgactggct cgccccgctc gcctgcgcgg cctggatgaa ctcgacggcg cggagcacct
     2941 gggcctcccc gcgggcgagc cgtgcgtagg acggccacca cacgacgatc accgccagcg
     3001 cggcgttccg cgcgctgggg ccgagggcgg tggcgatcgc catggcgagg atcagggcgg
     3061 gtacggcgag gaacacgtcg gcgagacgca tggtcgtttc gtcgatccag ccgccgagat
     3121 agccggcgat gaggcccacc gcgctgcccg cgaggccggc cgcgagcacc accgcgatgg
     3181 ccgggaccag cgacacccgc gccccgtgga tcacgcggct caggacgtcg cgacccaaat
     3241 agtcggtgcc gagggggtgg gccgcgctcg gcggccggag ccgctggccc ggctgggtct
     3301 tcaaagggtc ccacggcgcg agggcctccg ggaacgcggt gagggcgagg aagccgagca
     3361 gcacggcgcc gctcaccatc tggggcacgg tcgcgccgcg catcacgaga gccgcacccg
     3421 cggatcgagc acggagtaga ggacatcgac ggccaggttc acgagcgagt agaccagcgc
     3481 gaccaccagc gtcacgccca tcaccacgtt gaggtcgacg ttccgcgcgc cctcccaggc
     3541 gtagcggccg atgcccggcc acgcgaagat ggtttccgtg atgaccgcgc cgcccatcag
     3601 gaagccgacg cggaggccca tgacggtgac gaccgggatg gccgcgttct tcaccacgtg
     3661 ccgccagagc acggcgcgct cggcgaggcc cttgctgcgg gcggtccgga cgtgatcggc
     3721 gccgaggacg tcgagcaccg ccgagcgcgt gatgcgcgtc atctcggcga gggtgaggtt
     3781 gctgagcgcg agcgcgggca ggacgaggtg agcggcggcg tctcggaacg cccgcccgtc
     3841 gcgggcgagg aggctgtcga tcaggtagag gccggtgatg ccgggcggag cgggcaccat
     3901 cgagtcgagg cggccgccgt aagggagcca gccgagccag cggtagaaca ccagcagcag
     3961 cacgagcccc atcacgaaga cgggcaccga cagctgggcc accggcccgg cggtggcgac
     4021 agtgtcgatc gcgctgccgc gccggaccgc ggcgagcacc ccgagggcgc cgcccacgag
     4081 ga
//
LOCUS       DATMCV010000873         2678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_645637, whole genome shotgun sequence.
ACCESSION   DATMCV010000873 DATMCV010000000
VERSION     DATMCV010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645637"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VNU03_20025"
     CDS             complement(<1..573)
                     /locus_tag="VNU03_20025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76818.1"
                     /translation="MLEIRGLEAAYGDVPALSGVTLRVGAGEIVALLGPNGAGKSTLL
                     GCVAGLVRPRAGIISWDSENLLAVSPHLIVERGLAVVPEGRRLFGSMTVEDNLELGAF
                     SPRARAARRESLERVFALFPDLRTRRRQIVRALSGGQQQMVAVGRALMASPRLLMLDE
                     PSLGIAPLLVRRILDALVEINRAGVAVFLVE"
     gene            complement(566..1300)
                     /locus_tag="VNU03_20030"
     CDS             complement(566..1300)
                     /locus_tag="VNU03_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76819.1"
                     /translation="MALLEVERLTKGFGGVLAVSDVSLTLEAGELLGVMGPNGSGKTT
                     LFNLIAGALRPDRGRIRFQDRDIAGLSPHRVCARGIARTFQLVRPFAGLSALDNVRVG
                     CLYGRRRGADARTEALRLLALVGLEGRAATPAAALTLMDRKRLELARALATGPDLLLL
                     DEFMAGLNPTETATAMGLIRRLVADGLSVLMVEHIVWALMDLARRIVVLSAGEKIADG
                     PPAAVAADSRVVDVYLGVERPGAARA"
     gene            complement(1322..2503)
                     /locus_tag="VNU03_20035"
     CDS             complement(1322..2503)
                     /locus_tag="VNU03_20035"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWP76820.1"
                     /translation="MRMLAWLALALLGLSPVPAWAQSIKMGAVVPLTGRYGAGGAQIR
                     AGYEFAVERLNAGGGLTVGGVKIPVELVLLDDESDATKTVARMETLAAQGVIGYLGGF
                     GSDLHAAAASVAEKNRIPYLGVAFALQKIHQQGFRYLFSPFWKSPDIGHATQGLLELI
                     AAGERPKTVAIFQEKTDWGREMAAAWTEAGKTAGYQVALNGEYAPGAKDFSDLILKAK
                     AANVDVVLALPNPPDGMTIVKQMKELGFTPRLSFFIRAPDAQIWSQNLGKDGDFMVLA
                     AGWHHAMKGAGVKELNDAHQKKFGRPADPLVGPAYACVEILAAAVTRAGFPDREKVRD
                     AIAATSMNTVIGSVKFRPDGTGIVPQALLQWQSGKQELVWPKEAATATLAFPAPPFAK
                     R"
BASE COUNT          377 a          970 c          971 g          360 t
ORIGIN      
        1 ctccaccagg aagacggcca cgccggcccg gttgatctcg acgagcgcgt ccaggatccg
       61 ccgcaccagc aggggcgcga tgccgagcga cggttcgtcc agcatcagca ggcgcgggct
      121 cgccatcagg gcgcgcccca ccgcgaccat ctgctgctgg ccgcccgaga gggcgcggac
      181 gatctgccgg cgccgcgtgc gcaggtccgg gaagagcgcg aagacgcgct cgaggctctc
      241 ccgtcgcgcc gcgcgggccc tcggcgagaa ggcgcccagc tcgaggttgt cctccaccgt
      301 catggagccg aagaggcgcc ggccctccgg taccaccgcg aggccgcgct cgacgatcag
      361 gtgcggggag actgccagaa ggttctcact gtcccaggag atgatgcccg cgcggggccg
      421 taccagcccg gccacgcagc cgagcagggt ggacttgccc gcgccgttcg ggccgagcag
      481 cgcgacgatc tcgcccgcgc cgacccggag cgtgacgccg gagagcgcgg ggacgtcgcc
      541 gtaggccgcc tcgaggccgc ggatctcaag cacgcgcggc tcccgggcgc tccacgccga
      601 ggtagacgtc caccacccgg gaatcggcgg ccaccgcggc gggcgggccg tccgcgatct
      661 tctcgcccgc gctcagcacc acgatgcggc gcgcgaggtc catcagcgcc cacacgatgt
      721 gctccaccat caggaccgag agcccgtcgg cgaccagccg gcggatcagg cccatggccg
      781 tcgcggtctc ggtcgggttc aggccggcca tgaactcgtc gagcagcagc aggtccggcc
      841 cggtggcgag cgcgcgggcc agctcgaggc gcttccggtc catcagggtg agggcggcgg
      901 ccggcgtggc cgcgcggccc tcgaggccca ccagggcgag caggcggagg gcctcggtcc
      961 gggcgtcggc gccgcgccgc cggccgtaga ggcagcccac ccgcacgttg tccagcgcgg
     1021 agaggcccgc gaagggccgc acgagctgga acgtgcgcgc gatcccgcgg gcgcagacgc
     1081 gatgcgggga gagcccggcg atgtcgcgat cctggaagcg gatgcgcccc cgatccggac
     1141 ggagcgcgcc tgcgatcagg ttgaagagcg tggtcttgcc ggagccgttc ggtcccatga
     1201 ccccgagcag ctcgcccgcc tcgagggtga ggctcacgtc gctgaccgcg aggacgccgc
     1261 cgaagccctt ggtcagccgc tcgacctcca ggagggccac ggtcggctca ggcgccggag
     1321 ctcagcgctt ggcgaacggg ggcgccggga acgccagcgt cgcggtcgcc gcctccttcg
     1381 gccacaccag ctcctgcttg ccgctctgcc actgcagcag cgcctgcggg acgatgccgg
     1441 tgccgtcggg ccggaacttc acggagccga tcacggtgtt catgctcgtc gccgcgatcg
     1501 cgtcgcgcac cttctcgcga tccgggaagc ccgcgcgggt gaccgcggcg gccaggatct
     1561 ccacgcacgc gtaggccggg ccgaccagcg gatccgccgg gcgcccgaac ttcttctggt
     1621 gcgcgtcgtt cagctccttc accccggcgc ccttcatggc gtggtgccag cccgcggcca
     1681 ggaccatgaa gtcgccgtcc ttgccgaggt tctgcgacca gatctgggcg tcgggggcgc
     1741 ggatgaagaa ggagagccgg ggggtgaagc cgagctcctt catctgcttc acgatcgtca
     1801 tgccgtcggg cgggttgggc agggccagca ccacgtccac gttggcggcc ttggccttca
     1861 ggatcaggtc cgagaagtcc ttggcccccg gcgcgtactc gccgttcagc gcgacctggt
     1921 agcccgcggt cttgcccgcc tccgtccacg cggccgccat ctcgcggccc cagtccgtct
     1981 tctcctggaa gatggccacc gtcttgggtc gctcgccggc cgcgatcagc tcgagcaggc
     2041 cctgggtggc gtgaccgatg tcgggcgact tccagaacgg cgagaagaga tagcggaagc
     2101 cctgctggtg gatcttctgg agcgcgaagg ccacgcccag gtacgggatg cggttcttct
     2161 cggccaccga tgcggccgcc gcgtgcagat cggagccgaa gccgccgaga tagccgatca
     2221 cgccctgggc ggcgagggtc tccatgcgcg cgacggtctt ggtggcgtcc gactcgtcgt
     2281 cgagcaggac cagctcgacc ggtatcttga cgccgcccac cgtcaggccg ccgcccgcgt
     2341 tcaggcgctc caccgcgaat tcgtagccgg cgcggatctg ggcgccgccc gcgccgtagc
     2401 ggccggtcag gggaaccacc gcgcccatct tgatcgactg ggcccaggcg gggacgggcg
     2461 acagcccgag cagggcgaga gcgagccagg ccagcattct catcgggatt ctcctctcca
     2521 gattcgattc gcggtctcgg ccacgcggtg gagcttggcc tgctgctcgg cgggggtgat
     2581 ccggtttcct tccatggtgg aggcgaagcc gcactgcggg ctcaaggcca ggcgctcgag
     2641 tggcaccagc cgggccgcct cctcgacacg ggcgcgca
//
LOCUS       DATMCV010000874         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_314106, whole genome shotgun sequence.
ACCESSION   DATMCV010000874 DATMCV010000000
VERSION     DATMCV010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314106"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="VNU03_20040"
     CDS             complement(<1..1704)
                     /locus_tag="VNU03_20040"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HWP76821.1"
                     /translation="MAIRLLAAALLVGFLAGPALAAPPRAAGPPRVSVALISEGASIE
                     PGSPLWVGLRQRIAPGWHTYWLNPGDSGEAPSIEWSLPTGFGAEPIVWPQPERLPVGP
                     VMSYGYTKEVVLLTRVTVPGDARLGSRVTLRGRASWLVCEKTCIPEEADVALTLPVIE
                     GRPAPAVTASAIERARRAVPGPSPWPATLGATTKTVTVSVAAPRLAADRISDVWFFPA
                     RWGVIDHAAAQAVTIDADGITLRIARGALPDGPATDLDGVLVMRERLDQGLVTQAFAI
                     RATAAPASSAARAGLSLVVAIGLALAGGLILNLMPCVLPVLSVKAIALVSHAQTSSVV
                     LRRHGLAYTGGVLVSFAALAGALIALRAGGTEVGWGFQLQSPVVVGALAYLLFALGLS
                     LSGVLVLGTRLTGVGRGLAARPGYRGSFFTGALAAVAATPCTAPFMGVAVGYALTQPA
                     ATALLIFEALGLGLALPFLALSLVPAWRRLLPRPGPWMERLKQALAFPLYASVAWLVW
                     VVSRQAGPNGVALVLIGLLLIGFAAWLHQATRAGTSVRWRRAATVLALGCVAGALGLG
                     PL"
     gene            complement(1719..>2066)
                     /gene="msrB"
                     /locus_tag="VNU03_20045"
     CDS             complement(1719..>2066)
                     /gene="msrB"
                     /locus_tag="VNU03_20045"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWP76822.1"
                     /translation="DHHEPGIYVDVVSGEPLFSSTDKFDSGTGWPSFVQPIEPANVIE
                     HSDTSYGMRRVEVRSTEGDSHLGHLFPDGPRPTGMRYCINSAALRFIPVAKLEEEGYG
                     KYRALFEKVKPQS"
BASE COUNT          329 a          713 c          809 g          215 t
ORIGIN      
        1 cagcgggccg aggccgagcg cgccggccac gcagccgagc gcgagcacgg tggccgcgcg
       61 tcgccagcgc accgaggtgc ccgcgcgggt ggcctggtgg agccacgccg cgaagccgat
      121 caggagcagt ccgatcagga ccagcgccac cccgttcggc ccggcctggc ggctgaccac
      181 ccacacgagc caggccacgg aggcgtagag cgggaaggcg agcgcctgct tgagccgctc
      241 catccagggt cctgggcggg gcagcaggcg ccgccacgcg gggaccaggg agagggcgag
      301 gaatggcagg gccaggccga gtccgagggc ctcgaagatc agcagcgcgg tggccgccgg
      361 ctgggtgagc gcgtagccca ccgccacgcc catgaacggg gcggtgcagg gcgtcgcggc
      421 caccgcggcc agcgcgccgg tgaagaagga gccccggtag cccgggcggg ccgccagccc
      481 gcggccgacg ccggtcagcc gcgtgccgag cacgaggacg cccgagaggc tcaggccgag
      541 cgcgaagagc aggtaggcga gagcgccgac gaccaccggg gactggagct ggaagcccca
      601 gccgacctcg gtcccgcccg cgcgcagcgc gatcagggca ccggccagcg cggcgaacga
      661 gaccagcacg ccgcccgtgt aggcgagccc gtggcgccgg aggacgaccg acgaggtctg
      721 ggcatgggag accagggcaa tcgccttgac ggaaagcacg ggtagaacgc acggcatgag
      781 attcaggatg agcccgcccg cgagggccag accgatcgcg acgacgagag agagacccgc
      841 gcgggcggcg gaggatgcag gggcggcggt tgcccggatc gcgaaggcct gggtcaccag
      901 cccctggtcc agccgctcgc gcatcaccag gacgccgtcg agatcggtcg caggcccgtc
      961 cggcagcgca ccgcgggcga tgcgcaacgt gatgccgtcc gcgtcgatgg tgaccgcctg
     1021 ggccgccgcg tgatcgatga cgccccagcg agccgggaag aaccacacgt ccgagatgcg
     1081 gtcggcggcc aggcgagggg ccgcgaccga taccgtcacg gtcttggtcg tcgcgccgag
     1141 ggtggcgggc cacgggctcg ggcccggaac cgcgcggcgg gcgcgctcga tggccgatgc
     1201 ggtcaccgcc ggggccggac gtccctcgat gaccggcagg gtcagggcga cgtcggcctc
     1261 ctcggggatg caggtcttct cgcacacgag ccacgaggcc cgaccgcgca gcgtgacgcg
     1321 gctcccgagc cgggcgtcac ccggtacggt gacgcgggtc agcaggacga cctccttggt
     1381 gtagccgtag ctcatcaccg gccccaccgg cagccgctcg ggctgcggcc acacgatggg
     1441 ctccgcgccg aacccggtgg gaagagacca ttcgatgctc ggcgcctcgc cggagtcgcc
     1501 cgggttgagc cagtaggtgt gccagcccgg ggcgatgcgc tgccggagcc ccacccagag
     1561 cggagagccc ggctcgatgg aggcgccctc gctgatcagc gccaccgaga ccctgggagg
     1621 tccggccgcg cggggcgggg cggcgagcgc gggtcccgcc aggaacccga ccaggagcgc
     1681 ggcggcgagc aggcgaatcg ccatcgcggg gaagccggct acgactgcgg cttcaccttc
     1741 tcgaagagcg cgcggtactt gccgtaccct tcctcttcga gcttggcgac ggggatgaag
     1801 cggagcgctg ccgagttgat gcagtagcgc atcccggtcg ggcgggggcc gtcggggaag
     1861 aggtggccca ggtgcgagtc gccttccgtt gaccgcacct cgacccggcg catgccgtag
     1921 gaggtgtccg agtgctcgat cacgttggcc ggctcgatcg gctgcacgaa gctcggccag
     1981 ccggtgccgg agtcgaactt gtcggtcgag gagaagagcg gctcgcccga gaccacgtcc
     2041 acgtagatgc cgggctcgtg gtggtc
//
LOCUS       DATMCV010000875         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_982486, whole genome shotgun sequence.
ACCESSION   DATMCV010000875 DATMCV010000000
VERSION     DATMCV010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982486"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VNU03_20050"
     CDS             <1..447
                     /locus_tag="VNU03_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76823.1"
                     /translation="LVRAGELPSPPWPLTVAQAVALALRWPQLGTAAAVLAAAWPNAR
                     ARTRVAALLDRVGLTADARRRRDGLPAAAMGRLGLAQALAQRPRLLLLDEPLGRLPAD
                     DRPAAARLIDEIRAQGVSVLLTEREPAVAKTVADRVARLERGAVIG"
     gene            444..1508
                     /locus_tag="VNU03_20055"
     CDS             444..1508
                     /locus_tag="VNU03_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76824.1"
                     /translation="MSAEDVRGLAHLIGSVPLPTTEEVFRRLGATLGPLLSRMPDGET
                     GERRRWIYWQRTMLERHPAMEVDPDAGLLELRQWDGSLLRRTDLLRFRPGVDPDGVDF
                     PTGYADAARESWALFERLRREGALPASLRFQVCLPTPMSSAFMYVSPRAHDDYQRAYE
                     RALRRDLDLIVASIPPADLSIQWDICQEVLVCEGYFPSRPADYRERIFALLARLGDAV
                     PPGVEMGYHLCYGSPADQHLVMPKDAAILAELARAIVSGVKRRVDFLHLPVPRERDDT
                     AYLAPLRDLELPAGLRLYLGLLHHDDRAGDRRRIDVARDARPAFGVATECGWGRGEPA
                     RLAGLLESHRHAVDYLVTRR"
     gene            1505..2383
                     /locus_tag="VNU03_20060"
     CDS             1505..2383
                     /locus_tag="VNU03_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP76825.1"
                     /translation="MTDRTPGPASHTFFSQRLRLHYVDWGNPDKPPLLMIHGGRDHCR
                     NWDWTAAALRDDWHIIAPDLRGHGDSQWSADGSYSMTGYIYDLAQLIHGQRLGPVTII
                     AHSLGGNVALRYAGLYPETVARLVAIEGLGPSPKAWAERSAKSIAERLDEWIREQRGL
                     AGRLPRRYASIEDAFRRMQDENPHLTAEQARHLTVHGVNQNEDGTYSWKFDNYVRVFP
                     PYDMRGQDIKHLWSRIACPTLLLYGKESRSGNPADDGRAAAFRDATVIGIDRAGHWLH
                     HDRLDEFLRVVRDFLR"
     gene            complement(2380..>2719)
                     /locus_tag="VNU03_20065"
     CDS             complement(2380..>2719)
                     /locus_tag="VNU03_20065"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992758.1"
                     /note="catalyzes the cleavage of carbon-halogen bonds in
                     aliphatic compounds forming a primary alcohol and a
                     halide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HWP76826.1"
                     /translation="EAEMAVYRRPYLEPGESRRPTLTWPREIPVGGEPADVVAIADGY
                     ARWLATSQLPKLFINADPGTILTGAQREFCRRWPNQEEITVRGIHFIQEDSPREIGEA
                     VARFVERLPS"
BASE COUNT          340 a         1075 c          941 g          363 t
ORIGIN      
        1 ctggtccgcg ccggcgagct accctcgccg ccctggccgc tcacggtcgc gcaggcggtg
       61 gccctcgcgc tgcgctggcc gcagctcggc accgccgccg cggtcctcgc cgccgcgtgg
      121 cccaacgccc gcgcccgcac ccgcgtcgcg gcgctgctcg atcgggtcgg cctcaccgcg
      181 gatgcccggc ggcggcgcga cgggctgccc gcggcggcga tggggcgact gggcctggcc
      241 caggccctcg cccagcgccc tcgcctgctg ctcctggacg agccgctggg ccgcctgccg
      301 gccgacgacc ggccggccgc ggcccgcctg atcgatgaga ttcgcgccca gggggtgagc
      361 gtcctcctca ccgagcgcga gcccgcggtg gcaaagacgg tggcggaccg ggtggcgcgg
      421 ctcgagcgcg gcgcggtgat cggttgagcg ccgaggacgt ccgcgggctc gcccacctga
      481 tcggcagcgt gcccctgccc accaccgagg aggtgttccg ccgcctcggc gccacgctcg
      541 gcccgctgct ctcgcgcatg ccggacggcg agaccggcga gcggcggcgc tggatctact
      601 ggcagcgcac catgctcgag cgccacccgg ccatggaggt ggaccccgac gcggggctgc
      661 tcgagctgcg ccagtgggac ggctcgctcc tgcggcgcac cgatctgctg cgctttcgcc
      721 ccggcgtcga cccggacggg gtcgacttcc cgaccgggta cgcggacgcg gcgcgggagt
      781 cctgggcgct cttcgagcgg ctgcgccggg agggcgcgct gcccgcgagt ctgcgcttcc
      841 aggtctgcct gcccactccc atgtcgagcg cgttcatgta cgtgagcccg cgcgcccacg
      901 acgactacca gcgcgcctac gagcgcgcgc tccgccgcga cctggacctc atcgtggcga
      961 gcatccctcc cgccgatctc tcgatccagt gggacatctg ccaggaggtc ctcgtctgcg
     1021 agggctactt cccgtcgcgg cccgccgact atcgggagcg catcttcgcg ctgctcgcgc
     1081 gcctgggcga cgcggtgccg ccgggcgtgg agatgggcta tcacctctgc tacggcagcc
     1141 cggccgacca gcacctcgtg atgccgaagg acgccgcgat cctggccgag ctggcccgcg
     1201 ccatcgtctc gggcgtgaag cgccgcgtcg acttcctcca tctgccggtg ccgcgcgagc
     1261 gcgacgacac cgcctacctc gcgccgctgc gcgatctcga gctgcccgcg ggcctgcggc
     1321 tctacctggg cctgctccac cacgacgacc gcgcgggcga ccgccgccgt atcgacgtgg
     1381 cgcgcgacgc gcggcccgcc ttcggggtgg ccaccgagtg cggctggggc cgcggcgagc
     1441 ccgcgcggct cgcgggcctg ctcgagagcc accgccacgc cgtcgactac ctggtgaccc
     1501 gccgatgacc gatcgcacgc ccggccccgc ctcgcacacc ttcttctcgc agcgcctgcg
     1561 gctgcactac gtggactggg gcaaccccga caagccgccg ctgctgatga tccacggcgg
     1621 gcgcgatcac tgccgcaact gggactggac cgccgccgcg ctccgggacg actggcacat
     1681 catcgcgccg gacctgcgcg gccacggcga cagccagtgg tcggccgacg gcagctactc
     1741 gatgaccggg tacatctacg acctcgcgca gctcatccac ggccagcgcc tgggcccggt
     1801 caccatcatc gcccactcgc tcggcggcaa cgtggccctg cgctacgcgg gcctctaccc
     1861 cgagacggtc gcccggctcg tggcgatcga gggcctgggg ccgtcgccga aggcgtgggc
     1921 cgagcgctcc gccaagtcca tcgcggagcg cctggacgag tggatccgcg agcagcgcgg
     1981 gctggccggg cggctgcccc gccgctacgc ctccatcgag gacgccttcc gtcgcatgca
     2041 ggacgagaac ccgcacctca ccgcggagca ggcgcggcac ctcaccgtgc acggggtgaa
     2101 ccagaacgag gacggcacct acagctggaa gttcgacaac tacgtgcggg tgttcccgcc
     2161 ctacgacatg cgcgggcagg acatcaagca cctctggtca cgcatcgcgt gcccgacgct
     2221 cctgctctac ggcaaggaga gccgctccgg caacccggcc gacgacgggc gcgccgccgc
     2281 cttccgggac gccaccgtca tcggcatcga ccgcgccggc cactggctgc atcacgaccg
     2341 gctcgacgag ttcctccgcg tcgtccgcga cttcctccgc tagctcggaa gccgctccac
     2401 gaagcgcgcc accgcctcgc cgatctcccg cggcgagtcc tcctggatga agtggatgcc
     2461 cctcacggtg atctcctcct ggttgggcca ccgccggcag aactcgcgct gtgccccggt
     2521 gaggatcgtc cccgggtcgg cgttgatgaa cagcttgggc agctggctcg tggccagcca
     2581 ccgagcgtag ccgtccgcga tggcgaccac gtcggcgggc tcgcctccga ccgggatctc
     2641 ccgcggccag gtcagcgtgg gacggcgcga ctcgccgggc tcgagatacg ggcggcggta
     2701 gaccgccatc tccgcctcg
//
LOCUS       DATMCV010000876         6254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_452000, whole genome shotgun sequence.
ACCESSION   DATMCV010000876 DATMCV010000000
VERSION     DATMCV010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452000"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VNU03_20070"
     CDS             complement(<1..616)
                     /locus_tag="VNU03_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009805929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP76827.1"
                     /translation="MTTDLLETVKDGVAVLTLNRPDRLNAMSRPMLDALLEALPRLAD
                     DPAVGCVVLTGAGRGFCAGGDVKAMAEGNELGGQTMEEKAQALRSRMETSRWLHEMPK
                     PTIAMVRGPAAGAGLSLAMACDMRVASDTARLGTAFARVGYSGDFGGSYYLTQLVGTA
                     KARELYFTADLLDAQQALALGLLNRVVPDARLEEETMALASRLAR"
     gene            725..1612
                     /locus_tag="VNU03_20075"
     CDS             725..1612
                     /locus_tag="VNU03_20075"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWP76828.1"
                     /translation="MTVSPAAAAERALVPHTLEVDGITRSYHLHVPSAVGPAPAPLVL
                     VFHGGGGTGPGTERLTRFTALADREGFLVAFPEGVEKNWNDGREFTSSRAHRDHVDDV
                     AFVAVLIDAIGRAHAVDPRRVYATGISNGGIFSHYLAAHLSARLAAIAPVVGGIADPP
                     EAWLRPEQPVSVLMLQGTRDPLVPYHGGAVAFGRGKIIDTEEAARRWAALNGGREPVR
                     EPLPADGKDRCGGLRTIYPGGRDGSEVTLVRLDGGGHTWPGGAQYLPELLIGRVCRDF
                     DATVLIWDFFKTHPKPGGP"
     gene            complement(1623..3542)
                     /locus_tag="VNU03_20080"
     CDS             complement(1623..3542)
                     /locus_tag="VNU03_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP76829.1"
                     /translation="MPTPDTRATSEQESRRVAEQARAEVWEGRGFLRDLFLGQLSLDL
                     IHPFPLRPKERPEFTRFIDDLRAFLRDHVDPAAIDQSGEYPPDVLSGLRRLGAFGMKI
                     SRDYGGLGFTVSEYCRAMELVGSYDGNLAAVLSAHQSIGVPQPLKLFGTVEQKAKYLP
                     RCAAGAISAFALTESGVGSDPASLATTAERRDDHFVLNGEKLWCTNGTLAELLVVMAR
                     DPQSHRISAFIVETGWEGVTVEYRCRFMGLRALANGVISFRNVRVPRENLIGREGQGL
                     KIALATLNTGRLTLPAVCAGAAKRCLEICRGWAAARVQWGAPIWKHEAIGHRIADMAA
                     TTFAMDSVAILAAEMADRGGYDIRLEAAAAKEWNTVRAWELADQTLQIRGGRGYETEA
                     SLAGRGEAAIGVERMLRDLRINLIFEGSSEIMHLFMAREAVDKHLEVAGALVDPRRST
                     AEKRAALPRVAAFYARWYPLLWLRGLAAPARFGELGPLAPHLRFVERSARRLARATFH
                     GMLVFRAGMERRQAFLFRTVDIAMELFAMAAATTHAARLADGRYPEAERARELADLFC
                     RNARRKVRRLFRDLWRNDDALKGRVAAAVMGDRHTWLERGILELGLPVSAFRPQPFAR
                     TEPAREPEPAPATRA"
     gene            3592..4143
                     /locus_tag="VNU03_20085"
     CDS             3592..4143
                     /locus_tag="VNU03_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWP76830.1"
                     /translation="MLASPRQSPPRQEEIVIATDPIEQRVREVLDRLALHHEMVEIDP
                     QYADTAAFCAQYGFPIEQSANTIVVGSKKEPKRYCACVVRATRRLDVNHTVRKLLGVS
                     RLSFADPEETRALTGMMLGGVTVFALPPDLPIYVDEGLMALPWIILGGGSRSLKVKIT
                     PDVFRALPGATVVPGLVTAAVAE"
     gene            4140..6071
                     /locus_tag="VNU03_20090"
     CDS             4140..6071
                     /locus_tag="VNU03_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HWP76831.1"
                     /translation="MTPDAEGPQDATPATRARQRAVLDALPFGDVQDFEDAGRGFVAT
                     LPEVSVRNAQGRVVWSLGEYAFLEAAEAPATVNPSLWRQARLNMRHGLFQVTDRIYQV
                     RGFDLSNMTILEGERGLVVIDPLISTEVARAALALYREHRGDRPVTAVIYTHSHTDHY
                     GGVRGVVDEARVAAGEVEVIAPHRFMEEVVSEAVLVGTAMVRRGQFQFGATLPKGPRG
                     QVDAGLGKVTSRGTVTLIPPTRVVADPIEVHRIDGIEIVFQLAPETEAPAEMHMFYPA
                     LRALNLAENATHHLHNIYPLRGAQARDANAWAKYLDEARDRFGRGAQVAFAQHHWPVW
                     GTARILEYLGAQRDLYKYLHDQTLRLMNHGFKAAEIAERLALPRSLQSTWHVRGYYGT
                     LSHNAKSVYQRYLGWYDANPANLEPLPPVERGRKYVEYMGGAAAVLARAREDFARGEY
                     RFVAEATSHVVFADPANVEARRLGADALEQLGYAAESATWRNAYLLGAHELRRGRSDT
                     AARAPVSPDLVRAMSLDLFFDYLAVRLNGEKAEGHRFVLNWIFPDGDDPARGRRYVLT
                     LQNCALTYLAERQSEQPDATVTLGRPVLDRLVLRELTIAEAIEQGLVIIAGDRAPVAR
                     LFALLDDFALMFDVIEPRR"
BASE COUNT          853 a         2341 c         2225 g          835 t
ORIGIN      
        1 cgcgggccag gcgggaagcc agcgccatcg tctcctcctc gaggcgcgcg tcggggacga
       61 cgcgattgag gagccccagg gcgagcgcct gctgggcgtc cagcagatcg gcggtgaagt
      121 agagctcgcg ggccttggcc gtcccgacga gctgggtcag gtagtagctg ccgccgaagt
      181 cgcccgagta gccgacgcgg gcgaaggcgg tgccgagccg cgcggtgtcg gaggcgacgc
      241 gcatgtcgca cgccatcgcg agcgagagcc cggcgcccgc cgccggcccg cgcaccatcg
      301 cgatggtggg cttgggcatc tcgtgcagcc agcgcgacgt ctccatgcgg ctgcgcagcg
      361 cctgggcctt ctcctccatg gtctggccgc ccagctcgtt gccctcggcc atcgccttga
      421 cgtcgccgcc cgcgcagaag ccgcgtccgg cgccggtcag gaccacgcag ccgaccgccg
      481 ggtcgtccgc gaggcgcggc agagcctcca gcagggcgtc gagcatcggg cgcgacatgg
      541 cgttgagccg gtcgggccgg ttgagggtca gcacggcgac gccgtccttc acggtctcga
      601 gcaagtcggt ggtcatcggg gcccctcctt ccggagcagc ggcgattgta ccccttcgcc
      661 ggggcgcgca ggctgccgat caccgaggcg cgcgcggacg ctcgccctcg tcctggccgc
      721 gctcgtgacg gtctcccccg cggccgcggc ggagcgggcc ctcgtgcccc acaccctcga
      781 ggtcgacggg atcacccgga gctatcacct gcacgtgccg tcggcggtcg gccccgcccc
      841 cgccccgctc gtgctcgtct tccacggggg cggcggcacc ggcccgggca ccgagcggct
      901 cacgcgcttc acggccctcg ccgaccgcga ggggttcctg gtggcgttcc cggaaggcgt
      961 cgagaagaac tggaacgacg gccgcgagtt caccagctcg cgcgcgcacc gcgaccacgt
     1021 cgacgacgtg gccttcgtcg ccgtcctgat cgacgcgatc ggccgggcgc acgcggtgga
     1081 cccccggcgc gtgtacgcga ccggcatctc caacggcggg atcttctcgc actacctggc
     1141 cgcgcacctg tcggcgcgcc tcgcggccat cgccccggtc gtcggcggga tcgccgaccc
     1201 gcccgaggcg tggctccgac cggagcagcc ggtctcggtg ctgatgctcc agggcacgcg
     1261 cgacccgctg gtgccctatc acgggggagc ggtcgccttc ggccgcggca agatcatcga
     1321 taccgaggag gccgcccgcc gctgggccgc gctgaacggc gggcgcgagc cggtgcggga
     1381 gcccctgccc gccgacggca aggaccgctg cggcggcctc cggacgatct acccgggcgg
     1441 acgcgacggc agcgaggtga cgctggtgcg cctggacggc ggcgggcaca cctggcccgg
     1501 gggcgcccag tacctgccgg agctgctgat cgggcgcgtg tgccgggact tcgacgcgac
     1561 cgtgctgatc tgggacttct tcaagaccca ccccaagccc ggcgggccgt gatcgcgcgc
     1621 ggctaggccc gcgtggccgg ggcgggctcg ggctcccggg ccggctcggt gcgcgcgaac
     1681 ggctgcggcc ggaacgcgga gaccgggagc cccagctcga ggatgccccg ctcgagccac
     1741 gtgtgccggt cgcccatgac cgccgccgcg acgcggccct tcaacgcatc gtcgttgcgc
     1801 cacaggtcgc ggaagagccg ccgcaccttg cgccgcgcgt tccggcagaa gaggtcggcc
     1861 agctcgcggg cccgctccgc ctccgggtag cgcccgtcgg cgaggcgcgc ggcgtgagtg
     1921 gttgcggccg ccatcgcgaa cagctccatc gcgatgtcga cggtccggaa gaggaacgcc
     1981 tgccgccgct ccatcccggc ccggaagacc agcatcccgt ggaaggtcgc ccgcgcgagg
     2041 cggcgggcgc tccgctccac gaagcgcagg tggggcgcga ggggcccgag ctcgccgaag
     2101 cgcgcgggcg ccgcgagccc gcggagccag agtagcgggt accagcgcgc gtagaaggcg
     2161 gccacccggg gcagggccgc gcgcttctcg gccgtgctcc gccgcgggtc gacgagcgcc
     2221 cccgcgacct cgagatgctt gtcgaccgcc tcgcgcgcca tgaacagatg catgatctcg
     2281 ctcgagccct cgaagatcag gttgatgcgc aggtcgcgca gcatccgctc gacgccgatc
     2341 gcggcctccc cgcgcccggc cagcgaggcc tccgtctcgt agccgcggcc gccgcggatc
     2401 tgcagggtct ggtcggccag ctcccacgcc cgcaccgtgt tccactcctt ggccgcggcc
     2461 gcctcgagac ggatgtcgta gccgccgcgg tcggccatct cggcggcgag gatggccacc
     2521 gagtccatcg cgaaggtggt ggccgccatg tccgcgatcc ggtggccgat cgcctcgtgc
     2581 ttccagatcg gcgcgcccca ctgcacgcgg gccgcggccc agccccggca gatctcgagg
     2641 cagcgcttgg ccgctcccgc gcagaccgcg ggcagggtga ggcgcccggt gttgagggtg
     2701 gcgagcgcga tcttgagccc ttgcccctcg cgcccgatca ggttctcgcg cggcacccgc
     2761 acgttgcgga agctgatgac gccgttggcg agcgcgcgga ggcccatgaa gcggcagcgg
     2821 tactcgaccg tgacgccctc ccacccggtc tccacgatga acgcgctgat gcgatggctc
     2881 tgcggatcgc gcgccatcac caccagcagc tcggcgaggg tcccgttggt gcaccagagc
     2941 ttctcgccgt tgagcacgaa atgatcgtcg cgccgctcgg cggtggtggc gaggctcgcc
     3001 gggtccgagc ccaccccgga ctcggtcagg gcgaaggcgg agatggcgcc cgccgcgcag
     3061 cgcggcaggt acttggcctt ctgctcgacg gtcccgaaga gcttgagcgg ctgcggcacc
     3121 ccgatggact ggtgcgcgga cagcaccgcg gcgaggttgc cgtcgtagct gcccaccagc
     3181 tccatggccc ggcagtactc ggacaccgtg aagccgaggc cgccgtagtc ccgcgagatc
     3241 ttcatcccga aggcgcccag tctccggagc ccggacagga cgtctggtgg atactcgccg
     3301 ctctggtcga tcgcggccgg gtcgacgtgg tcgcgcagga acgcgcgcag gtcgtcgatg
     3361 aagcgcgtga actccggccg ctccttgggc ctcaggggaa acgggtggat caggtcgagg
     3421 gacagctggc ccaggaagag gtcgcgcagg aagccgcgcc cctcccagac ctccgcgcgg
     3481 gcctgctcgg ccactcgccg ggattcctgc tcgctcgtcg cccgcgtgtc cggtgtcggc
     3541 atgggatcct cccgtctcca tcgattctag cggattcgcg ggggcggcgg tatgctcgcc
     3601 tcgccgcgtc aatccccacc tcgacaggag gagatcgtca tcgccaccga cccgatcgag
     3661 cagcgagtgc gcgaggtcct cgaccgcctc gcgctgcatc acgagatggt cgagatcgac
     3721 ccgcagtacg ccgacaccgc cgccttctgc gcccagtacg gcttccccat tgagcagtcc
     3781 gccaacacca tcgtcgtggg ttccaagaag gagccgaagc gctactgcgc ctgcgtggtg
     3841 cgggcgacgc gccggctcga cgtcaaccac accgtccgca agctcctggg cgtctcccgc
     3901 ctctccttcg ccgatcccga ggagacgcgc gccctgaccg gcatgatgct gggcggggtc
     3961 accgtgttcg ccctgccgcc cgacctgccg atctacgtgg acgaggggct gatggcgctg
     4021 ccctggatca tcctgggcgg gggcagccgc tccctcaagg tgaagatcac gcccgacgtc
     4081 ttccgcgccc tgcccggcgc caccgtggtc ccgggcctcg tcaccgcggc cgtcgcggaa
     4141 tgacgcccga cgcggagggc ccgcaggacg ccaccccggc cacccgcgcg cgccagcggg
     4201 cggtgctcga cgcgctgccc ttcggcgacg tccaggactt cgaggacgcg ggccgcggct
     4261 tcgtggccac cctgcccgag gtctccgtgc gcaacgccca gggtcgcgtg gtgtggagcc
     4321 tcggcgagta cgcgttcctc gaggccgcgg aggctcccgc caccgtgaac ccgagcctct
     4381 ggcggcaggc gcggctcaac atgcgccacg ggctcttcca ggtcaccgac cgcatctacc
     4441 aggtccgcgg gttcgacctc tcgaacatga cgatcctcga gggtgagcgc ggcctcgtcg
     4501 tcatcgatcc gctgatctcg accgaggtgg cgcgcgccgc cctcgccctc taccgcgagc
     4561 accgcggcga ccgcccggtg accgcggtca tctacaccca cagccacacc gaccactacg
     4621 gcggggtccg cggggtcgtg gacgaggcgc gggtggccgc gggagaggtc gaggtcatcg
     4681 ccccgcaccg cttcatggag gaggtcgtct cggaggcggt gctggtcggg accgcgatgg
     4741 tccgccgcgg ccagttccag ttcggcgcca ccctgcccaa ggggccgcgc ggccaggtgg
     4801 acgcggggct cggcaaggtg acctcgcgcg gaaccgtcac cctgatcccg ccgacccgcg
     4861 tggtggccga cccgatcgag gtccaccgga tcgacgggat cgagatcgtc ttccagctgg
     4921 ctcccgagac cgaggccccc gccgagatgc acatgttcta cccggccctg cgcgccctca
     4981 acctggccga gaacgcgacc caccacctcc acaacatcta tccgctccgc ggggcgcagg
     5041 cgcgcgacgc caacgcgtgg gccaagtacc tcgacgaggc gcgggatcgg ttcgggcgcg
     5101 gggcccaggt ggccttcgcc cagcaccact ggccggtgtg gggcactgcg cggatcctcg
     5161 agtacctggg cgcgcagcgc gacctctaca agtacctcca cgaccagacc ctgcgtctca
     5221 tgaaccacgg cttcaaggcg gccgagatcg cggagcgcct ggcgttgccc cggagcctgc
     5281 agtcgacctg gcacgtgcgc ggctactacg gcaccctctc ccacaacgcc aagagcgtct
     5341 accagcgcta tctcggctgg tacgacgcca accccgcgaa cctcgagccg ctgcctccgg
     5401 tcgagcgcgg acggaagtac gtcgagtaca tgggcggggc cgccgcggtg ctcgcgcggg
     5461 cgcgcgagga cttcgcccgc ggcgagtacc gcttcgtggc cgaggcgacg agccacgtgg
     5521 tgttcgcgga tcccgcgaac gtggaggcgc ggcgcctcgg cgcggacgcg ctcgagcagc
     5581 tcggctacgc cgccgagtcg gcgacctggc gcaacgccta cctcctcggc gcccacgagc
     5641 tgcggcgggg ccgatccgac accgcggcgc gggctccggt gagccccgac ctcgtccggg
     5701 ccatgagcct cgatctcttc ttcgactacc tcgcggtgcg cctgaacggc gagaaggcgg
     5761 agggccaccg cttcgtcctg aactggatct tccccgacgg cgacgatccg gcgcggggcc
     5821 ggcgctacgt gctcaccctg cagaactgcg ccctgaccta cctcgccgag cgccagagcg
     5881 agcagcccga cgcgacggtg acgctcgggc ggccggtgct ggaccggctc gtgctgcgcg
     5941 agctcaccat cgcggaggcg atcgaacagg gtctggtgat catcgcgggt gatcgggccc
     6001 cggtcgcccg gctcttcgcg ctcctcgacg acttcgcgct gatgttcgac gtgatcgagc
     6061 cgcggcgctg aggcggaacg gcccctactc gcgcagggcg cccgcgagca gcccgcgcac
     6121 gaggtaccgc tggaaggcga ccacgatcag gatgggcggc aggctcgcca gcaacgaggc
     6181 ggccgcgatg tcgccccacg gcacctcgaa gacgccggga aagagcgcca gggccaccgg
     6241 caccgtgcgg ctcg
//
LOCUS       DATMCV010000877         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_180945, whole genome shotgun sequence.
ACCESSION   DATMCV010000877 DATMCV010000000
VERSION     DATMCV010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180945"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1581
                     /locus_tag="VNU03_20095"
     CDS             100..1581
                     /locus_tag="VNU03_20095"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HWP76832.1"
                     /translation="MRTGSEYRDALHDGRRVWVMGEGLVEDVTVHPATRPMVDEYAAW
                     YDRHHDPTWQDVLLTAPDERGARRPWAFAVPRTSTDLRAMGRSYAATIFLTAGNMTHT
                     PGYGNLIALGIQDVVRQRKVSPEQGASAAAYRDLLARTGRFLTFSAGAATIGYRLRED
                     PDQRAALRIVRETGAGLVLSGKVGMHTSPVYAEDVYVGAHSGVDRDGHRATFIVPVNA
                     PGVTVLCRKISARHANPFLAPLSSRFDELDGQLWLEEVLVPWERVFLTEASPDPIAAW
                     CFWHQLYAWLAKAEFTLGLALACAHAMGLRDHEPTIEHLMDLVVDVQTVRSCQTAAEL
                     DPDVSEEGYCIPGRAHVAAGSIAMQKARQRMAEILRIVPGSSLVVAPSDKDLTSPEVG
                     AGLEESFGGGEYTALQRAALLQLAADHVASALDGRESAFELHANGGLVAWRARLRRAF
                     PDYNALANGVLRALSLDMPAIDLDSLRAIALPQRRPVTPPRRA"
     gene            complement(1661..>2133)
                     /locus_tag="VNU03_20100"
     CDS             complement(1661..>2133)
                     /locus_tag="VNU03_20100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP76833.1"
                     /translation="LVLTHHHMDHAGGLRAYASAGATLVVGKGTTEHYRRVLAAPYTR
                     NPDLSPQDLSRTPIVEVAEKYSIIDSQREVQVYAIEPNPHAQGLLIGFVPDAKLAYAT
                     DIWSPGAGPLPDKLNPGLAALVAGVKKAGIEPAKFAGGHGSTADYAPLAALEGK"
BASE COUNT          276 a          766 c          785 g          306 t
ORIGIN      
        1 cggcacggcc acccggggat gctggaggac tgggcggacg tcttcgccgg cgtctagctc
       61 acgggcgtct agtacgggga cgtccaggag gttcgagcgg tgaggaccgg cagcgagtat
      121 cgcgacgccc tgcacgacgg ccggcgggtc tgggtcatgg gcgaggggct ggtcgaggac
      181 gtcacggtcc acccggccac ccgtccgatg gtcgacgagt acgccgcgtg gtacgaccgc
      241 caccacgatc cgacgtggca ggacgtgctc ctgaccgcgc cggacgagcg aggcgcgcgc
      301 cggccgtggg ccttcgcggt gccgcggacc tcgaccgatc tacgcgcgat gggccggagc
      361 tacgccgcga cgatcttcct caccgcgggc aacatgaccc acaccccggg ctacgggaac
      421 ctcatcgcgc tcggcatcca ggacgtggtc cggcagcgca aggtctcgcc cgagcagggc
      481 gccagcgccg cggcgtatcg cgacctgctc gcccgcaccg gccgcttcct cacgttctcg
      541 gccggcgcgg ccaccatcgg ctaccggctc cgcgaggatc ccgaccagcg ggcggcgctc
      601 cgcatcgtcc gcgagaccgg cgccggtctc gtgctgagcg gcaaggtggg catgcacacg
      661 agcccggtct acgcggagga cgtctacgtc ggcgcccact ccggggtcga tcgcgacggc
      721 caccgggcca cgttcatcgt gccggtgaac gcacccggcg tgaccgtgct gtgccgcaag
      781 atctcggccc gccacgcgaa cccgttcctc gcgccgctca gcagccgctt cgacgagctg
      841 gacggacagc tctggctcga ggaggtgctg gtgccgtggg agcgcgtgtt cctcaccgag
      901 gcgtcgcccg atccgatcgc ggcgtggtgc ttctggcatc agctctacgc ctggctcgcg
      961 aaggccgagt tcacgctggg gctcgcgctg gcgtgcgccc acgcgatggg cctccgggac
     1021 cacgagccga ccatcgagca cctgatggac ctggtggtgg acgtccagac cgtccgcagc
     1081 tgccagaccg cggccgagct ggacccggac gtctcggagg agggctactg catccccggc
     1141 cgcgcccacg tggcggcggg cagcatcgcg atgcagaagg cacggcagcg catggcggag
     1201 attctgagga tcgtgcccgg ctcgtcgctc gtggtggcgc cgtcggacaa ggacctgacc
     1261 tcgcccgagg tgggcgccgg cctcgaggag tcgttcggcg ggggcgagta caccgcgctg
     1321 cagcgcgcgg ccctgctcca gctggccgcc gaccacgtgg cctcggccct cgacggccgc
     1381 gagtccgcgt tcgagctgca cgccaacggc gggctcgtgg cctggcgcgc ccgtctccgg
     1441 cgcgcgttcc ccgactacaa cgcgctcgcc aacggcgtgc tgcgcgccct gagcctcgac
     1501 atgcccgcga tcgacctgga ctcgctccgt gcgatcgccc tgccgcagcg gcggccggtc
     1561 accccgccgc ggcgggcata gctggggccc ctcaccctgc gcccctcacc ctgcgtccct
     1621 caccctgccc tctccccggg ggggagaggg tggggggagg ttatttgcct tcgagggcgg
     1681 cgaggggggc gtagtcggcg gtggagccgt ggccgccggc gaacttggcg ggctcgatgc
     1741 cggccttctt cacgcccgcg accagcgcgg cgagacccgg gttgagcttg tccggcagcg
     1801 gccccgcgcc cgggctccag atgtcggtcg cgtaggcgag cttggcgtcg gggacgaagc
     1861 cgatcagcag gccctgcgcg tgcgggttgg gctcgatcgc atagacctgc acctcgcgct
     1921 ggctgtcgat gatcgagtac ttctccgcga cctcgacgat cggggtgcgc gagagatcct
     1981 gcggcgagag atcggggttg cgcgtgtacg gcgcggccag cacgcggcgg tagtgctcgg
     2041 tggtgccctt gcccaccacg agcgtggccc ctgcgctcgc gtaggcccgg agtccgcccg
     2101 cgtgatccat gtggtgatgg gtgagcacga ggt
//
LOCUS       DATMCV010000878         3180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_452131, whole genome shotgun sequence.
ACCESSION   DATMCV010000878 DATMCV010000000
VERSION     DATMCV010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452131"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..503
                     /locus_tag="VNU03_20105"
     CDS             120..503
                     /locus_tag="VNU03_20105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP76834.1"
                     /translation="MADLTGVHVLVVEDTDDSREMLRVSLEYCGALVTTAASATEAKK
                     VLEHLRPHVLVSDIAMPDNGLELIREVMTVAGEKGVHIPAIAVTAYHDRREELLAAGF
                     AELVEKPLNPIALCGVIRRHGQLET"
     gene            complement(933..1490)
                     /locus_tag="VNU03_20110"
     CDS             complement(933..1490)
                     /locus_tag="VNU03_20110"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HWP76835.1"
                     /translation="MPGRRARRPGERRPQPTLDEKAARLAAFDLLARKSWSGRELTRR
                     LRRRGAPAEIAQAVVADLTTRGYVDDQAFARWWAEARARGRRVGSRRLRQELLAKGIP
                     REMTDAAIAAAFEEVGEADRALEAARRRLPGLLRGKGERAAARLADYLLRRGYPAPAV
                     RRAVRTLLTDRAGDLPSADEDDGGV"
     gene            complement(1498..2709)
                     /locus_tag="VNU03_20115"
     CDS             complement(1498..2709)
                     /locus_tag="VNU03_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWP76836.1"
                     /translation="MELQQLLSETAQRKASDLHLKSGSKPILRIHGHLEVQDDLPMVT
                     REFIDKMAESLLGERRVGMLLEGREMDLGYMLPGIGRFRCNLFLAMGEVRGVFRHVPE
                     RIPAFQELHLPKVLERLSMERRGMILVTGITGSGKSTSLASMIDFMNRSRNDHIVTIE
                     DPIEFVHQDKKCVMSQREIGHDSMNFAQALRAALREDPDIMLVGELRDVETMEVGLHA
                     AETGHLVLSTLHTLNAAETINRMISMFPPHQEDQIRGQLASVIQGIVSQRLVVRADGR
                     GRVPAVEIMIATGLIRDCIREADKTPQIPSVIAAGTAQYGMQTFDQSLLQLYREELVT
                     YETARDAATNPDDFDLKVKGIFSTGEMTWEHPGGSSTASTHTPPAQPASLSGVAAPAA
                     PAARSPFGKRA"
     gene            complement(2722..>3180)
                     /locus_tag="VNU03_20120"
     CDS             complement(2722..>3180)
                     /locus_tag="VNU03_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76837.1"
                     /translation="IREKIGVMFGSPETTTGGRALKFYSSIRLDIRRQDTIKNGTEAV
                     GVRTKVKVVKNKLSPPFREAEFDVIYGEGISKEGSVLDLASEHNVVEKSGTWYTYKSE
                     RIGQGRENAKRYLKENPKILVEVEAKVRDALGLRPIGGAAPAPAPEKAAK"
BASE COUNT          462 a         1073 c         1109 g          536 t
ORIGIN      
        1 cagacggagt tcttaccggg tcgttctgga gcacttcccc agcgtactgg ccctccgcat
       61 gccgcagcag caggtacgac cattggcacg cgccgtgctc tgaggcacgg aggcaagcca
      121 tggcggacct tactggcgtc cacgtactgg tcgtcgagga caccgacgac tcgcgcgaaa
      181 tgctgcgcgt ctccctggag tactgcgggg cgctcgtcac gacggccgcg tcagccaccg
      241 aagcgaagaa ggtcctggag cacttgcgac ctcacgtcct ggttagcgac atcgccatgc
      301 cggacaacgg cctggagcta atccgcgaag tcatgacggt cgcaggggag aagggcgtcc
      361 acatcccggc gatcgcggtc accgcgtacc acgatcggcg cgaagagctg ctggctgccg
      421 ggttcgcgga acttgtcgag aagcccctca acccaatcgc gctctgcggg gtgatccgcc
      481 gccacgggca gctggagacg tagccctcct gtggccgacg acgtcatcga gagtcgggtg
      541 cgcgagtggt tgcgcgacca ccaaggcagc gcgtactgcg cgaggtgcgt cgcgagaggc
      601 ctccacatcg atgtcgctct cgtgcaaacc gccatggacg acctcaatac acgacaggtc
      661 ttctcgcgtg gcccctgcgc gtgcgggacg atggggctgg cctacgggtg gtctgcgggc
      721 ggcatgaggc gatagagccc gaaggagcac cctcacgagg gcggctcatc cccttcgggc
      781 gattgccggg cgacccgtag cgcccggtgc ccctcagtct acttcggatg ccagccacga
      841 gctctgaggg aaacgagcct gcagtggtgg gtcagtctgg gcgctgtggt cgtttccctg
      901 tggcctcagc gaccgcctgc tctagtgcga tattatacgc cgccgtcgtc ctcgtcggcg
      961 gacgggaggt cgcccgcgcg atcggtgagc agcgtgcgga ccgcccggcg tactgccggc
     1021 gccggatagc cacgcctcag caggtagtcg gcgaggcgcg cggccgcccg ctcgcctttg
     1081 ccccgcagca gcccgggaag acgccggcgc gcggcctcga gggctcgatc ggcctcgccg
     1141 acctcctcga aggcggcggc gatggccgcg tccgtcatct cgcgggggat gcccttggcg
     1201 agcagctcct ggcggagccg gcggctgccc acccggcggc cgcgggcccg cgcctcggcc
     1261 caccagcgag cgaaggcctg gtcgtccacg taaccgcggg tggtgaggtc ggcgaccacc
     1321 gcctgggcga tctccgcggg ggcgccccgg cggcggaggc ggcgggtcag ctcccgcccg
     1381 ctccaggact tccgggcgag aagatcgaag gccgccagtc tggcggcctt ctcatcgagt
     1441 gtcggctgcg ggcgccgctc cccgggacgg cgggcgcggc gccccggcac cgggagccta
     1501 ggcccgcttg ccgaaggggc tccgcgcggc cggcgcggcg ggcgcggcga ccccgctgag
     1561 gctcgcgggc tgggccgggg gggtgtgggt ggacgcggtg ctgctcccgc ccggatgctc
     1621 ccacgtcatc tcgccggtgg agaagatgcc cttcaccttg agatcgaagt cgtccgggtt
     1681 ggtggccgcg tcgcgggcgg tttcgtacgt gaccagctcc tcgcgataga gctgcaggag
     1741 cgactggtcg aaggtctgca tgccgtactg ggcggtgccc gccgcgatca cgctcggaat
     1801 ctgcggggtc ttgtccgcct cccggatgca gtcccggatg aggccggtgg cgatcatgat
     1861 ctccaccgcg ggcacgcggc cccggccgtc ggcgcgcacc acgaggcgct gcgagacgat
     1921 gccctggatg accgaggcga gctgaccccg gatctggtcc tcctggtgcg gggggaacat
     1981 ggagatcatc cggttgatgg tctccgctgc gttcagcgtg tgcagcgtgg agagcaccag
     2041 gtgacccgtc tccgccgcgt ggagccccac ttccatggtc tccacgtcgc gcagctcgcc
     2101 gaccagcatg atgtcggggt cttcacgaag ggcggcgcgc agggcctggg cgaagttcat
     2161 cgagtcgtgg ccgatctcgc gctggctcat cacgcacttc ttgtcctggt gcacgaactc
     2221 gatcgggtcc tcgatggtga cgatgtggtc gttccggctc cggttcatga agtcgatcat
     2281 ggaggcgagg ctggtcgact tgccggagcc ggtgatcccg gtgaccagga tcatcccgcg
     2341 acgctccatc gacagccgct cgagcacctt gggcaggtgc agctcctgga aggcggggat
     2401 gcgctcgggc acgtgccgga agacgccacg cacctcgccc atggcgagga agaggttgca
     2461 gcggaagcga ccgatcccgg gcagcatgta gcccaggtcc atctcgcgtc cctccagcag
     2521 catgccgacc cggcgctcgc ccagcaggct ctcggccatc ttgtcgatga actcgcgggt
     2581 gaccatgggc aggtcgtcct gcacctcgag atggccgtgg atcctgagga tcggcttcga
     2641 acccgacttc aggtgcaggt ccgaggcctt gcgctgcgcc gtctccgaca gcagctgctg
     2701 gagttccatc gtcgctcgct cctacttggc cgccttctcc ggggccgggg ccggcgccgc
     2761 ccctccgatt ggcctgaggc cgagggcgtc gcggactttg gcctcgactt cgaccaagat
     2821 cttgggattt tccttcaggt accgcttggc gttctcgcgg ccctgaccga tccgctcact
     2881 cttataggtg taccaagtac cggatttttc gacaacattg tgctccgagg cgaggtccag
     2941 gaccgagccc tccttggaga tgccctcccc gtaaatgacg tcgaactcgg cctcccggaa
     3001 gggcggagag agcttgttct tcaccacttt gaccttggtc cggaccccga ccgcctcggt
     3061 gccattcttg atggtgtcct ggcgccggat gtccaggcgg atcgaggagt agaacttgag
     3121 cgcccggccc ccggtggtgg tctcggggga gccgaacatg accccgatct tctcccggat
//
LOCUS       DATMCV010000879         4480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_646095, whole genome shotgun sequence.
ACCESSION   DATMCV010000879 DATMCV010000000
VERSION     DATMCV010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646095"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VNU03_20125"
     CDS             complement(<1..532)
                     /locus_tag="VNU03_20125"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWP76838.1"
                     /translation="MSAQISLPIFPLPDLTFFPHTMLPLHIFEARYRAMITDCLSRDR
                     RLAVVGLRPGYEASYEGRPAVYEVMGVGRIVQWERMATGRFNLLLQGECRARIDRELP
                     ADTLYRMVAATPLDDTGVDAPAVAALTGRVRSRAAQILATVGRSGVELQATLDALTEP
                     GRLCDQIASTLIPSSST"
     gene            complement(538..1665)
                     /locus_tag="VNU03_20130"
     CDS             complement(538..1665)
                     /locus_tag="VNU03_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWP76839.1"
                     /translation="MATPTAPTEHIVLDALRAVRDPDQGQDIVSLGLVKEIRIQDAEV
                     SFTLAFAGQAPATKAALHSSASKAVSQLPGVSRVSVKMGSAGAARPQAGAPPAHAHGH
                     GQAAPQAQAAEFIPEVKHTIAVSSGKGGVGKSTVAVNLALALKQSGAQVGLVDVDVYG
                     PDVPLMMGAKGRPGMFDNKIIPVEAHGIKIMSIGLLVAEREALVWRGPMIHSAVQQFL
                     RDVNWGPLDYLVFDMPPGTGDAQLSLSQVVPLSGVVMVTTPQEVALLDVRKALGMFRK
                     LNVPILGIVENMSYFAAPDTGRRYPIFGEGGGLRVAEEFGVPLLGQIPLEMDTRAGGD
                     AGVPITVGQPDSAQAKAFRTIAAAAVARVEAVAGLKLPDLR"
     gene            1927..2391
                     /locus_tag="VNU03_20135"
     CDS             1927..2391
                     /locus_tag="VNU03_20135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWP76840.1"
                     /translation="MSHFATGVTIITAWDAEHRPTGLTASSFTSVSLHPPLILVCVSQ
                     KAQSYPAIKSAGQFAVNILCTGQEAASRRFATAPTASGDEKFVGLGYRPGAFGLPILD
                     EALAAIECTVVQAHPAGDHTIFVAQVEAADCRDDAGREPLLYFRGKYSRLHP"
     gene            2408..3490
                     /locus_tag="VNU03_20140"
     CDS             2408..3490
                     /locus_tag="VNU03_20140"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWP76841.1"
                     /translation="MPVPLSRPPVDDEIKAAVLAAIDSRQYILGPECKAFEQEFARYL
                     GRKHAVLTSSATAAIWMVLKAFGVKAEDEVLVPSHTAFPTVEAICFAGATPIFVDVDE
                     SYTVDLADATAKVSPRTVGFVPVHLYGHPADLDGIRRLCAERSLWLLEDCAQAHGAAW
                     RGRKVGTFGRAGVFSFYPSKNLTVMGDGGLLVTDDDDVAARCRRLRDHGRVNKDVHAE
                     IGFNLRFNDIQAAVGRVLLRRLDAMNDHRRALAARYTAGLAGLPLGLPVETPGARHVY
                     HLYVVRTPERDALARFLKERGIATGIHYPVPSHKQPAVEPFEPPALPVTERLVREILT
                     LPISAGHTEAEIDEVVAAVKEFFRTR"
     gene            3524..>4480
                     /locus_tag="VNU03_20145"
     CDS             3524..>4480
                     /locus_tag="VNU03_20145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP76842.1"
                     /translation="MNILQIYPKADYFTGAAMQLRDLAAGLAARGHHVTVATRPSAQW
                     ERACAAARVRHAPVPMRGSLDLRSAWTLARLIRDQHVEVVHCQKGRARSLALLAGLVT
                     TIPVMILNRGVSFPVDRWTRLGYTTPRVSAIVAVCESIKRGLVTSGVPAAKIDVIYSG
                     TDLGRFHPGVDGRRIRAELGLAPDHALVTQVGIRSWRGNDDVLEAMARVCHAAPHARL
                     LFVGAPPPRIPTLRDRAFRRGIGHAVSLLGHREDIPEILAASDLVVDASYAGLGLTGS
                     LREALAVETPVIGTDIEGHPELIVEGETGLLVPPRNPDALAQA"
BASE COUNT          635 a         1679 c         1541 g          625 t
ORIGIN      
        1 gcgtagagga gctgggtatc agcgtggacg cgatctgatc gcacagccgc ccgggctcgg
       61 tcagcgcgtc gagggtcgcc tgcagctcca ctcccgagcg gcccacagtg gccaggatct
      121 gggcggcccg gctccggacc cggccggtca gcgccgccac cgcgggcgcg tcgactccgg
      181 tgtcatcgag cggcgtggcc gcgaccatgc gatagagcgt gtccgcgggc agctcgcggt
      241 cgatccgcgc gcgacactcg ccctgcagca gcaggttgaa ccggccggtg gccatccgct
      301 cccactgcac gatgcgcccg acccccatga cctcgtagac cgccggccgc ccctcgtagg
      361 aggcctcgta gcccggcctc agccccacca ccgcgagccg ccggtccctc gagagacagt
      421 cggtgatcat cgcgcggtag cgcgcctcga agatgtggag cgggagcatc gtgtgcggga
      481 agaaggtgag gtccggcagg gggaagatcg gcagcgagat ctgagccgac acccggatca
      541 gcgcagatcg gggagcttca gcccggccac cgcctccacg cgcgcgacgg ccgcggccgc
      601 gatggtccgg aacgccttcg cctgcgccga atcgggctgg ccgaccgtga tcggcacgcc
      661 cgcgtcgccg cccgcgcgcg tgtccatctc gagcgggatc tggcccagca gcggcacccc
      721 gaactcctcg gccacccgca ggccgccgcc ctcgccgaag atggggtagc ggcggccggt
      781 gtccggtgcc gcgaagtagc tcatgttctc cacgatgccg aggatcggca cgttcagctt
      841 gcggaacatg ccgagcgcct tgcgcacgtc gaggagcgcc acctcctgcg gggtcgtcac
      901 catgaccacc ccggagagcg gcaccacctg cgagagcgag agctgggcgt cgccggtgcc
      961 gggcggcatg tcgaagacca ggtagtcgag cgggccccag ttgacgtcgc ggagaaactg
     1021 ttgcaccgcg gagtggatca tcggcccgcg ccagacgagg gcctcccgct cggccaccag
     1081 caggccgatc gacatgatct tgatgccgtg ggcctcgacc gggatgatct tgttgtcgaa
     1141 catccccggg cgccccttgg cgcccatcat cagcggcacg tcgggtccgt agacgtccac
     1201 gtcgaccagg ccgacctggg cccccgactg cttgagcgcc agcgccaggt tcaccgccac
     1261 cgtggatttg cccacgccac ccttgccgga ggagaccgcg atggtgtgct tgacctccgg
     1321 gatgaactcg gcggcctggg cctgcggcgc ggcctgcccg tggccgtggg cgtgcgccgg
     1381 cggcgcgccc gcctgcggac gggcggcgcc cgcggagccc atcttcacgc tcacccggct
     1441 gacgcccggc agctgcgaga cggccttcga ggcgctgctg tggagcgcgg ccttggtcgc
     1501 gggcgcctgg ccggcaaacg ccagcgtgaa ggacacttcc gcgtcctgga tgcgaatctc
     1561 cttcaccagg ccgagcgaga cgatgtcctg accctgatcg ggatcgcgga ccgcgcggag
     1621 ggcgtcgaga acgatgtgct cggtcggagc ggtgggcgtc gccatggtgt gcgcgcagta
     1681 tactcggcgc ctacccggga cgccaccacc ggggcccgat cctcacctct caaccgccgc
     1741 gaacgtcgtc gctcctagcg cccggaagcc gagcgcgaac cgggcagccg gtctcgatca
     1801 cggcagccgc gaagtcgtcc atgcacccag tcttccggtg cggtccaagc ccttggagaa
     1861 cttgaaaaat cgagcaggaa ttgctacaac ggtcaggtgc tcggacccga cgacttccgc
     1921 cgtgcgatga gtcatttcgc caccggggtg accatcatca ccgcgtggga cgccgagcac
     1981 cgcccgaccg ggctcaccgc cagctccttc acgtcggtgt cgctccaccc gccgctcatc
     2041 ctggtctgcg tgtcccagaa ggcgcagagc tatccggcga tcaagtcggc cggccagttc
     2101 gcggtgaaca tcctctgcac cgggcaggag gcggcctcac gccggttcgc caccgcaccg
     2161 accgcaagcg gcgacgagaa gttcgtcggc ctgggctacc gcccgggcgc gttcggcctg
     2221 ccgatcctgg acgaggccct ggccgcgatc gaatgcaccg tggtccaggc ccacccggcc
     2281 ggcgatcaca cgatcttcgt ggcccaggtc gaggcggccg actgccgcga cgacgcgggg
     2341 cgcgagccgc tcctgtactt ccgcggcaag tactcccggc tccacccgtg acccgaggac
     2401 tcagctgatg cccgtccccc tctcccgccc gccggtcgac gacgagatca aggccgcggt
     2461 gctcgcggcc atcgattcgc ggcagtacat cctcgggccg gagtgcaagg ccttcgagca
     2521 ggagttcgcg cgctacctgg gccgcaagca cgcggtgctg acgagcagcg ccaccgccgc
     2581 gatctggatg gtgctcaagg ccttcggggt caaggccgag gacgaggtgc tcgtgccctc
     2641 gcacaccgcc ttccccaccg tggaggcgat ctgcttcgcg ggcgcgacgc cgatcttcgt
     2701 cgacgtcgac gagtcctaca ccgtcgatct ggcggacgcg accgcgaagg tgtcgccccg
     2761 gaccgtcggg ttcgtcccgg tgcacctcta cggccacccg gccgacctcg acggcatccg
     2821 ccgcctctgc gcagagcgga gcctgtggct gctcgaggac tgcgcgcagg ctcacggggc
     2881 cgcctggcgg gggcgcaagg tcggcacctt cgggcgcgcg ggcgtgttct ccttctatcc
     2941 gtccaagaac ctcaccgtga tgggcgacgg cggcctgctg gtcaccgacg acgacgacgt
     3001 cgcggcgcgc tgccggcggc tgcgcgacca cggccgggtc aacaaagacg tgcacgccga
     3061 gatcggcttc aacctgcgct tcaacgacat ccaggctgcg gtcggccgcg tgctgctgcg
     3121 ccggctcgac gccatgaacg accaccgccg tgcgctcgcc gcgcgctaca ccgcgggcct
     3181 ggccggcctg cccctcggcc tgccggtgga gacgccaggc gcccgccacg tctaccacct
     3241 ctacgtggtg cgcacccccg agcgcgacgc gctcgcccgc ttcctcaagg agcgcggcat
     3301 cgcgaccggc atccactacc cggtgcccag ccacaagcag cccgcggtcg agccgttcga
     3361 gcccccggcc ctgccggtga ccgagcgtct cgtccgcgag atcctgaccc tgccgatctc
     3421 ggccggccac acggaggccg agatcgacga ggtggtcgcc gcggtgaagg agttcttccg
     3481 tacccggtga ccgaccggca ttgaggaacc gcgggggctg ctcgtgaaca tcctccagat
     3541 ctaccccaag gcggactact tcaccggggc ggcgatgcag ctccgcgacc tcgccgcggg
     3601 cctcgccgcg cgcggccatc acgtgacggt ggccacccgg ccgagcgcgc agtgggagcg
     3661 cgcctgcgcg gcggcccggg tgcgccacgc gccggtgccc atgcgcggga gcctcgacct
     3721 gcgctcggcg tggaccctgg cccggctgat ccgcgaccag cacgtggagg tcgtgcactg
     3781 ccagaagggc cgcgcccgct cgctcgccct cctcgccggg ctcgtcacca cgatcccggt
     3841 gatgatcctg aatcgcgggg tgagcttccc ggtggatcgc tggacgcgcc tcggctacac
     3901 caccccgcgg gtctccgcga tcgtggcggt gtgcgaatcg atcaagcggg ggctggtgac
     3961 ctccggggtg ccggcggcca agatcgacgt gatctactcc ggtaccgatc tggggcgctt
     4021 ccatcccggc gtggacggcc ggcgcatccg ggccgagctc gggctcgccc ccgaccacgc
     4081 cctcgtcacc caggtgggca tccgctcgtg gcgcggcaac gacgacgtgc tggaggcgat
     4141 ggcccgcgtc tgccacgccg ccccgcacgc gcgcctgctc ttcgtgggcg cgccgccgcc
     4201 gcgcatcccg accctgcgcg atcgcgcctt ccggcgcggc atcggccacg cggtctcgct
     4261 gctcggccat cgcgaggaca tcccggagat cctcgccgcc agcgacctgg tggtggacgc
     4321 ctcctacgcc gggctcgggc tgaccggctc gctgcgcgag gccctcgcgg tggagacccc
     4381 ggtgatcggc accgacatcg aggggcaccc cgagctgatc gtcgagggcg agaccgggct
     4441 gctggtgccg ccccgcaatc cggacgcgct ggcccaggcg
//
LOCUS       DATMCV010000880         2991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_779829, whole genome shotgun sequence.
ACCESSION   DATMCV010000880 DATMCV010000000
VERSION     DATMCV010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779829"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1988
                     /locus_tag="VNU03_20150"
     CDS             24..1988
                     /locus_tag="VNU03_20150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWP76843.1"
                     /translation="MTTSRGMVPADLTRIRFVSDAQVSPDGRVVAFVVTTLSEERDEY
                     LSQIWLVDGAGGEPRRFTTGPRRDTAPRWSPDGTRLAFVSEREGKKKGQLHVMPAAGG
                     EPIRLTDLRHGVSAPAWSPDGTRLAFVSRVGGWVEPESEEEKRKSRPPRVITSLKYRF
                     NGEGFTYDRRPQIFTVAADGGEPRQVTEGDYDHADPAWSPDGRTLAFTSARHDERDHD
                     DVSDIWLVSAEGGTPHRVTEADRPAAHPAFSPSGDAIAYLGRATRNAFGRNIRLFTVP
                     AKGGASECRTDALDRSCGPLGVAPIWSADGRALTIAAEDQGSLGLYRVEPGAGPPRRV
                     VGGERVVGGYSASRDGTVLAFTASEPGAPAEVFVCGPDGSGERKLTDLNRAFTREVAL
                     ARPERFRFERAGFTVDAWIVKPHGFEPGRRYPLLVNVHGGPHAQYGYPFFDEFQVQTG
                     AGYGVLYTNPRGSQGYGEAFTSAVVGDWGGGDAADMEAALDEALRRFDWIDRERLGLL
                     GGSYGGFMTSWIVGHSKRFRAACSERAVNAQTSMFGTSDIGFLFNQVELGGVLPWEDF
                     GKYVERSPLTYAKDITTPLLILHSEDDLRCPIEQGEQLFVALKTLRREVVFVRFPDEN
                     HELTRSGKPRHRLERFRILLEWFAKYLRSA"
     gene            complement(2001..2933)
                     /gene="cysK"
                     /locus_tag="VNU03_20155"
     CDS             complement(2001..2933)
                     /gene="cysK"
                     /locus_tag="VNU03_20155"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HWP76844.1"
                     /translation="MRIANDVTELVGNTPLVRIRRLTEGAKGQVVAKLEFYSPAHSVK
                     DRIGVSMIDAAEKAGLIKPDTIILEPTSGNTGIGLAFVCAARGYKLTLLMPDSMSKER
                     RMLLRAYGAELILTPAADGMPGAIKRAEEMAKADSRYFIPQQFQNPANPEIHRRTTAE
                     EIWRDTDGQVDILVAGIGTGGTITGVGEVIKARKPEFRCVAVEPDASAVLSGGAKGPH
                     PIQGIGAGFIPDILNTKIYDEIIRVKNDDAFAFARRAAREEGLLVGISSGAALWAAVQ
                     VARRPESAGKLIVVIIPSFGERYLSTPLYADLAD"
BASE COUNT          396 a         1107 c         1048 g          440 t
ORIGIN      
        1 gcccgcggta tgatgcgctc gcgatgacca cctcccgcgg catggtgccc gccgatctca
       61 cccgcatccg cttcgtcagc gacgcccagg tctcgcccga cggccgcgtc gtcgccttcg
      121 tcgtcaccac gctctccgag gagcgggacg aatatctctc ccagatctgg ctcgtggacg
      181 gagccggggg cgagccccgc cgcttcacca ccggcccccg ccgggacacc gcgccgcgct
      241 ggtctcccga cggcacgcgg ctggcgttcg tctccgagcg cgagggcaag aagaaggggc
      301 agctccacgt gatgcccgcc gccgggggcg agccgattcg cctcaccgat ctgcggcacg
      361 gcgtgagcgc tcccgcgtgg tcgcccgacg ggacccgtct ggccttcgtc tcgcgggtcg
      421 gcggctgggt ggagcccgag tccgaggagg agaagcggaa gtcgcggccg ccgcgcgtga
      481 tcacctcgct caagtaccgg ttcaacggcg agggcttcac ctacgaccgg cgcccgcaga
      541 tcttcacggt ggccgccgac ggcggcgagc cgcgccaggt gaccgagggc gactacgatc
      601 acgccgatcc ggcctggtcg ccggacggcc gcaccctcgc cttcacctcc gcgcgccacg
      661 acgagcgcga ccacgacgac gtgagcgaca tctggctcgt ttccgccgaa ggcggcaccc
      721 cgcaccgggt gacggaggcc gaccggccgg ccgcccatcc cgcgttctcc ccgtcggggg
      781 acgcgatcgc ctacctcggg cgcgcgaccc ggaacgcgtt cggccggaac atccgcctct
      841 tcaccgtgcc cgccaagggc ggcgcgtccg agtgccgcac cgacgccctc gaccgctcgt
      901 gcgggccgct cggggtggcc ccgatctgga gcgccgacgg ccgcgcgctc acgatcgccg
      961 cggaggacca ggggagcctc gggctctacc gcgtcgagcc gggcgcgggc cccccgcggc
     1021 gggtggtggg cggcgagcgc gtcgtcggcg gctactccgc ctcgcgcgac ggcacggtcc
     1081 tcgcgttcac cgcgagcgag ccgggcgcgc ccgccgaggt gttcgtgtgc ggccccgacg
     1141 ggagcggcga gcggaagctc accgatctca accgggcctt cacccgggag gtcgccctgg
     1201 cgcgccccga gcggttccgc ttcgagcgcg ccggcttcac cgtcgacgcg tggatcgtga
     1261 agccccacgg cttcgagccg ggccggcgct atcccctgct ggtcaacgtc cacggcggcc
     1321 cgcacgccca gtacggctat ccgttcttcg acgagttcca ggtgcagacg ggcgcgggct
     1381 acggcgtgct ctacacgaac ccgcgcggca gccagggcta cggcgaggcc ttcacctccg
     1441 cggtggtggg cgactggggc ggcggcgacg ccgccgacat ggaggccgcg ctggacgagg
     1501 cgctgcgccg cttcgactgg atcgaccgcg agcggctcgg cctcctgggg ggcagctacg
     1561 gcgggttcat gacgagctgg atcgtcgggc actcgaagcg cttccgggcc gcctgctcgg
     1621 agcgcgcggt caacgcgcag accagcatgt tcggcaccag cgacatcggc ttcctgttca
     1681 accaggtgga gctgggcggg gtgctgccgt gggaggactt cggcaagtac gtcgagcggt
     1741 ccccgctgac ctacgccaag gacatcacca ccccgctcct gatcctccac tccgaggacg
     1801 acctgcgctg tccgatcgag cagggcgagc agctcttcgt ggccctcaag accttgcggc
     1861 gcgaggtggt cttcgtgcgc ttccccgacg agaaccacga gctgacccgc tcgggcaagc
     1921 cgcgccaccg cctcgagcgc ttccgcatcc tgctcgagtg gttcgcgaag tacctccgct
     1981 ccgcctagtc ggccctgcgg ctagtcggcg agatccgcgt agagcggggt gctgaggtac
     2041 cgctctccga acgaggggat gatcaccacg atcagcttcc ccgcgctctc cggacgacgc
     2101 gccacctgga ccgcggccca cagcgcggcc cccgaggaga tgccgaccag cagcccctcc
     2161 tcgcgcgcgg cccgccgcgc gaaggcgaag gcgtcgtcgt tcttcacccg gatgatctcg
     2221 tcgtagatct tggtgttgag gatgtcgggg atgaagccgg ccccgatccc ctggatgggg
     2281 tgcggcccct tggcgccgcc ggagaggacc gcggaggcgt cgggctcgac cgccacgcag
     2341 cggaactcgg gcttgcgcgc cttgatcacc tcgcccaccc cggtgatggt gccgccggtg
     2401 ccgatgccgg ccaccaggat gtcgacctgg ccgtcggtgt cccgccagat ctcctccgcg
     2461 gtggtgcgac ggtggatctc gggattcgcc gggttctgga actgctgcgg gatgaagtag
     2521 cgggagtcgg ccttggccat ctcctccgcg cgcttgatcg cgccgggcat gccatcggcg
     2581 gcgggggtca ggatcagctc ggctccgtag gcgcgaagga gcatgcgccg ctccttgctc
     2641 atgctgtccg gcatgagcag cgtgagcttg tagccacggg ccgcgcagac gaaggccagc
     2701 ccgataccgg tgttgccgct cgtcggctcc aggatgatgg tgtcgggctt gatcaggccg
     2761 gccttctcgg ccgcgtcgat catcgacacc ccgatccggt ccttcacgct gtgggccggg
     2821 ctgtagaact cgagcttggc caccacctgc cccttcgcgc cctcggtcag ccggcggatc
     2881 cggaccagcg gggtgttgcc gaccagctcc gtgacgtcgt tggcgattcg catgcgcggg
     2941 ctcctcccgg ccacactcta caccggcggt ctaccgccgg gagcccggac g
//
LOCUS       DATMCV010000881         2415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_582315, whole genome shotgun sequence.
ACCESSION   DATMCV010000881 DATMCV010000000
VERSION     DATMCV010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582315"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1349)
                     /locus_tag="VNU03_20160"
     CDS             complement(63..1349)
                     /locus_tag="VNU03_20160"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWP76845.1"
                     /translation="MRVFSRHAVRALFLHRQHLGRPRAHGLTPHRLTRFVEDVGGIQM
                     DSINVVERAHYLTVWSRFGPYERARLDRLVYRRRLFLEYWAHAACLVPASMLPWWKRA
                     MLDYRVRHTGWSGWLRKNTRLLATVTDMIRAGGPMGHAELEGRRPPRGRSGWWDWRPV
                     QHALHYLWMTGVLTIHSRRHFQKRFDLLERVLPATAGSEAVSADAFLCWHIERSLHAM
                     GAAIELDLTHYLTYPRGATSERRAALRAMIAEGLVTEIAVEGSTARWLALARDLPALG
                     RAGRLPAASHGTTLLAPFDSLLWYRDRVSRVFGFDYRIEVYTPGPKRVHGYYTLPILH
                     DGQLVGRLDAKAHRGDRHLEVRHVHLEPWAARGQAPPAGGPSIDRDAMLAGIAEAATS
                     LARFVEADRVTVARVTPGRLRGPLVRAMRGTEALGA"
     gene            1374..2123
                     /locus_tag="VNU03_20165"
     CDS             1374..2123
                     /locus_tag="VNU03_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76846.1"
                     /translation="MGTRRGLLIAGLLASFIGPAPAHAVEYRLEVANLWETALYPFAK
                     PAELSDGASGPGLLKLEASLDQGAIPHAVVLRDRTLRWSSESVAQAYGTVRVLAEIKA
                     AGDGTRWDEVRWEGKPGERSVWMVLPSGSGRPQRLYRTVLNGAGAPRQFMPYVPVNGS
                     RSAAVKYPLNFLWSYERRGTIWDRYVSKSLDLSDGLGAVVGENFNQMFPDTVYLIVQQ
                     GPEPTTYKAVLLWRDPAYNFEAPGRQGTPIR"
BASE COUNT          353 a          840 c          901 g          321 t
ORIGIN      
        1 tgcgctcgcg acggtgacca ggaggccgaa ggcgacgatc gcgaggtcgc gggccggatg
       61 cctcatgccc cgagtgcttc cgtcccgcgc atcgcccgca cgagaggccc gcgcaggcgc
      121 cccggcgtca cccgggcgac ggtgacgcgg tcggcctcga cgaagcgtgc gagcgaggtg
      181 gccgcctcgg cgatgcccgc cagcatcgcg tcccgatcga tcgacggccc gcccgcgggc
      241 ggcgcctgcc cgcgcgcggc ccacggctcg aggtgcacgt gccgcacctc gagatgccgg
      301 tcgcctcggt gggccttggc gtcgagccgg ccgacgagct ggccgtcgtg gaggatcggc
      361 agcgtgtagt agccgtggac tcgcttcggc cccggggtgt agacctcgat gcggtagtcg
      421 aacccgaaga cgcgggagac ccggtcgcgg taccagagca acgagtcgaa cggggcgagc
      481 agggtggtgc cgtgcgaggc cgcgggcagc cggcccgcgc ggccgagcgc gggcaggtcg
      541 cgggccagcg cgagccaccg cgcggtcgag ccctccaccg cgatctcggt caccaggccc
      601 tccgcgatca tcgcgcgcag tgcggcgcga cgctccgacg tggcgccgcg cgggtaggtc
      661 aggtagtggg tgaggtcgag ctcgatcgcg gcgcccatcg cgtggagcga ccgctcgatg
      721 tgccagcaca ggaacgcgtc cgccgatacc gcctcgctgc cggcggtcgc ggggagcacc
      781 cgctccagca ggtcgaagcg cttctggaag tggcgccggg agtggatggt cagcacgccg
      841 gtcatccaga ggtagtgcag cgcgtgctgc accggccgcc agtcccacca gccgctgcgg
      901 ccgcgcggcg gccgtcgccc ctcgagctcc gcgtggccca tcggcccgcc cgcccggatc
      961 atgtcggtga cggtggcgag cagccgggtg ttcttgcgca gccagcccga ccagccggtg
     1021 tgtcgcacgc ggtagtcgag catcgcgcgt ttccaccacg gcagcatcga ggcgggcacc
     1081 agacaggcgg cgtgcgccca gtattccagg aagaggcggc ggcggtagac gagacggtcg
     1141 agccgcgccc gctcatacgg cccgaagcgg ctccacaccg tgaggtagtg ggcgcgctcc
     1201 accacgttga tcgagtccat ctggatgccg cccacgtctt cgacgaagcg cgtcaaacgg
     1261 tggggagtca ggccatgggc gcgcgggcga ccgaggtgct gccggtgcag gaagagcgcc
     1321 cgcacggcgt ggcgcgagaa gacacgcatg gcgcttgaga gtatcatccg tccatgggga
     1381 cacgccgggg actcctgatc gcgggcctgc tcgcctcctt catcgggccc gcaccggccc
     1441 atgccgtgga gtaccggttg gaggtcgcga acctctggga gaccgcgctc tatcccttcg
     1501 ccaagccggc cgagctgtct gacggcgcct ccggccccgg gctcctgaag ctcgaggcga
     1561 gcctggacca gggagccata ccgcacgcgg tcgtcctgcg agaccgcacg ctgcggtggt
     1621 cgagcgaatc ggtggcccag gcctacggca cggtgcgggt gctggccgag atcaaggcgg
     1681 ccggtgacgg cacgcgctgg gacgaggtgc gctgggaggg caagccgggc gagcggagcg
     1741 tgtggatggt gctgcccagc gggagcgggc ggcctcagcg cctctaccgg accgtgctca
     1801 acggcgcggg cgcgccgcgg cagttcatgc cgtacgtgcc ggtgaacggc tcccgcagcg
     1861 cggcggtgaa atatcccctt aacttcctct ggtcctacga gaggcgcggg acgatctggg
     1921 accggtacgt ctcgaagagc ctcgacctga gcgacggcct cggtgcggtg gtgggggaga
     1981 acttcaacca gatgttcccg gatacggtct acctgatcgt gcaacagggc cccgagccga
     2041 cgacctacaa ggcggtgctg ctctggcgcg atccggcgta caacttcgag gcgcccggca
     2101 ggcagggcac cccgatccgc tagccgcgcc cgcgcctcac cggcgtttca ccggacggac
     2161 gccgcgaaga tccgcaggta gttgccgccc acgatcttgg cggtgtccgc cggcgtgaag
     2221 ccgccccgca gcatcgcgtc caccaggccg gtgaacttgt cgtagtctgg gaagaggccg
     2281 ccgctccccg agctcctcgg gaggctcctc aggggttgac cgcgccgtgc tcacgcagga
     2341 aggtgagccc gcctcgcgcg tcgtccaggt ccagctccag caccccgggc aggttggcga
     2401 aggcgggatg ccgcg
//
LOCUS       DATMCV010000882         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_844191, whole genome shotgun sequence.
ACCESSION   DATMCV010000882 DATMCV010000000
VERSION     DATMCV010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844191"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VNU03_20170"
     CDS             complement(<1..1211)
                     /locus_tag="VNU03_20170"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP76847.1"
                     /translation="MANDTAGAFEHTEAMFPKLTAAQLARLLPIGRRRRFAAGEVVFE
                     RGSAKRAFYLLLAGRVEIATPSREGDVRLTIHGIGEFTGEVDMISGRQSLVRASALDA
                     AELLEIDLADLRHIVQTDAELSELFLRAFVLRRAHLIANTLGGVVLIGSGHSADTLRI
                     KAFLVRNGHPHVYLDVERDAGVDALLEHFQIGVAEIPVLMCRDRDRALRNPSNAEVAT
                     ALGFNSAPDDGHVHDLVVVGAGPSGLAAAVYGASEGLDVLVLETSAPGGQAGSSSRIE
                     NYLGFPMGISGQDLAGRALLQAEKFGASLTVARAAVGLTCADRQLRVRCADGGTVQGH
                     TVVVASGAAYRKLAVPELPRFEGVGVYYGATRVEAQLCEGEEIVVVGGGNSAGQAAMY
                     LSGIAGHVHLLV"
     gene            1285..>2732
                     /locus_tag="VNU03_20175"
     CDS             1285..>2732
                     /locus_tag="VNU03_20175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HWP76848.1"
                     /translation="MQSAQLVLALLIAVAALVTIARRLGIAYPIFLVIGGLVLGLVPG
                     TPRVGIDPDLIFLVVLPPLLYIASFYTPLRSLRANLGSIGSLAIGLVIASALAAGATA
                     HALMPGMPWAIAIALGAIVAPPDEIAATAIAARLAVPRRIVTILEGESLLNDATALTI
                     YGIAIAVAVGGAFRPSSGVLTFTGALVGGTAIGLAVGWIIGQVRQRIDDTPVEITISL
                     LTPYAAFLPAQQLGASGVIATVAAGLYLGRRASHMAGPDVRLTGRAVWETITFLLNGI
                     VFIVTGLEVPLLLRNLAPATAIRLVGIGIAVSLVLVAVRALWIFSTSSLACLVRPETR
                     PPRLFAQSLVLSWAGMRGVISLAVVLALPPTLPDGGPYPTREALLIVTLTVIVFTLLG
                     QGLTLPWLIRRLRLGSDPELHEEEALARQQLVEAALRRIDQLYPVWPGHRPLLDRLRE
                     TYEHRSEHVERQRDAAGSGEEDREIIEHREIHR"
BASE COUNT          346 a         1050 c          922 g          414 t
ORIGIN      
        1 accaggaggt ggacgtgccc cgcgatcccg gacaggtaca tcgcggcctg cccggccgaa
       61 ttgccgcctc ccaccaccac gatctcctcg ccctcgcaga gctgcgcctc gactcgcgtg
      121 gcgccgtagt agaccccgac cccctcgaac cggggaagct cgggcaccgc aagcttgcga
      181 taggccgcgc cgctggccac caccacggtg tggccctgca ccgtcccgcc gtcggcgcac
      241 cggacccgca gctgccggtc ggcacaggtg aggcccaccg ccgcccgcgc caccgtcagg
      301 ctcgccccga acttctcggc ctggaggagc gcgcggccgg ccaggtcctg gcccgagatg
      361 cccatcggga accccaggta gttctcgatg cgagagctcg agccggcctg gccgcccgga
      421 gcgctggtct cgagcaccag cacgtccagc ccctcggagg ccccgtagac cgccgcggcc
      481 aggcccgagg gccccgcgcc caccaccacg aggtcgtgca cgtgcccgtc gtcgggcgcg
      541 ctgttgaagc cgagcgcggt cgccacctcc gcgttgctcg ggttccgtag cgcccggtcc
      601 cggtcccggc acatcagcac cgggatctcc gcgacgccga tctggaagtg ctcgagcagc
      661 gcgtccacgc ccgcgtcccg ctccacgtcc aggtagacgt gcggatgtcc gttgcggacc
      721 aggaaggcct tgatgcgcag ggtatccgcc gagtggcccg acccgatcag cacgacgccg
      781 cccagggtgt tggcgatgag gtgcgcgcgc cgcaggacga acgcgcgcag gaacagctcg
      841 ctcagctcgg cgtccgtctg cacgatgtgc cggaggtccg ccaggtcgat ctcgagcagc
      901 tccgccgcgt cgagggcgct ggcccggacc aggctctggc gcccggagat catgtcgact
      961 tccccggtga actccccgat cccgtgaatg gtgaggcgca catccccctc ccgcgagggg
     1021 gtcgcgatct ccacgcggcc ggccagcagc aggtagaacg cgcgcttggc cgagccgcgc
     1081 tcgaagacca cctcgcccgc cgcgaaccgg cgacgccggc cgatcggcaa caggcgcgcg
     1141 agctgcgccg cggtgagctt cgggaacatc gcttcggtgt gctcgaaggc gcccgccgtg
     1201 tcgttcgcca tactcgtggc catagtgctt gaaggatagc atcgccgtcc agcgcccgga
     1261 cccgtgtgct aggctgcgaa cgccatgcag agcgcccagc tcgtcctggc tctgctgatc
     1321 gcggtcgccg cgctggtcac gatcgcgcga cggctcggca tcgcctatcc gatcttcctg
     1381 gtgatcggcg ggctcgtgct ggggctcgtg ccgggtacgc cgcgggtcgg catcgatccg
     1441 gacctgatct tcctcgtggt cctgcccccg ctgctctata tcgcctcgtt ttacactccg
     1501 ctgcggagtt tgcgggcgaa cctgggctcg atcggctcgc tcgccatcgg cctggtgatc
     1561 gccagcgcgc tcgcggccgg cgcgaccgcg cacgccctga tgcccggcat gccgtgggcc
     1621 atcgccatcg ccctgggggc gatcgtggcc cctcccgacg agatcgcggc caccgcgatc
     1681 gccgcgcgcc tggcggtgcc gcggcggatc gtgacgatcc tggagggcga gagcctgctc
     1741 aacgacgcga ccgcgctcac catctacggc atcgcgatcg cggtcgcggt cggcggcgcg
     1801 ttccgcccgt ccagcggcgt gctcaccttc acgggcgccc tggtgggggg caccgcgatc
     1861 ggcctggcgg tgggttggat catcggacag gtccgccagc ggatcgacga tacgccggtg
     1921 gagatcacga tctccctgtt gacgccctac gccgccttcc tccccgccca gcagctgggc
     1981 gcttccgggg tgatcgcgac ggtcgcggcc gggctctacc tcggccgccg cgcctcgcac
     2041 atggcgggcc ccgacgtccg cctgaccggc cgcgcggtct gggagacgat caccttcctc
     2101 ctgaacggca tcgtcttcat cgtgaccggt ctcgaggtgc ccctgctcct gcgcaacctc
     2161 gcccccgcga ccgccatccg gctcgtcggc atcggcatcg cggtgagcct ggtcctggtc
     2221 gcggtccggg ccctctggat cttctcgacc tcgtccctcg cgtgcctggt ccgcccggag
     2281 acccgcccgc cccggctctt cgcgcagtcc ctggtgctct cgtgggccgg gatgcgcggg
     2341 gtcatctccc tcgcggtggt gctggctctg ccgccgaccc tgcccgacgg cgggccctac
     2401 cccacgcgcg aggcgctcct gatcgtgacc ctgaccgtca tcgtgttcac cctcctgggc
     2461 caggggctga ccctgccgtg gctgatccgc cggctccggc tcggctccga cccggagctg
     2521 cacgaggaag aggccctcgc gcgccagcag ctcgtcgagg cggcgctgcg ccggatcgat
     2581 cagctctacc ccgtgtggcc gggccatcgc ccgctgctcg atcggctgcg tgagacgtac
     2641 gagcatcgct cggagcacgt cgagcggcag cgggacgcgg ccggctccgg ggaggaagac
     2701 cgggagatca tcgagcaccg cgagatccat cg
//
LOCUS       DATMCV010000883         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_844296, whole genome shotgun sequence.
ACCESSION   DATMCV010000883 DATMCV010000000
VERSION     DATMCV010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844296"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VNU03_20180"
     CDS             <1..291
                     /locus_tag="VNU03_20180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP76849.1"
                     /translation="GIPVVVTSQAAQALGAEAGRDLLVADAPREFASQILQLLRDQEA
                     AAEMGARGQLLVATKYAWSVLTAPVHELVESIAKVGPPPPAPKPLAGLRGSR"
     gene            288..1388
                     /locus_tag="VNU03_20185"
     CDS             288..1388
                     /locus_tag="VNU03_20185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP76850.1"
                     /translation="MKTRRVESLAGFEALSPAWATVVRESGQTSPFLSHEWFTCCWRG
                     AGAGRRPEAILVEDTTGPVGLVPLTHWRGNLHRLPVRFVGMLNSPDTPYVDWLMVGRP
                     EPVVDAVLTELMRRRDWDVFSLAGLPADSLTLKALETVLPGRFRTLRLPALPSPYVDV
                     SGSWNAFWTGTSQRFKKTVRSIRNRLQKAGEIRVEEHRDLAPGSPILDEVIDVSRRSW
                     KAVENVAIANMPGMRDFFRELTACASRNGWLRLWLLRLDGRVVATEYQLEAAGRVHAL
                     RADFDGTLPSDLSPGTHLSEEIIRRLFERPGIHEYDMGPGDNAYKQRWATGTHEHVRL
                     LIFRPGAVGATLHTMANRIVPALRRLRPGSAR"
     gene            1385..>2224
                     /locus_tag="VNU03_20190"
     CDS             1385..>2224
                     /locus_tag="VNU03_20190"
                     /inference="COORDINATES: protein motif:HMM:NF024927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWP76851.1"
                     /translation="MSLIAGLVYRDPARPVEPEVLAALVEGSSVVHPRESRLDTGAAL
                     VAGGPGASAEACGPVVLAADMDVTNVTELLRQRGVTTLPELMHDLYESEGRRFPLRLR
                     GGFALALWYPERRRLVLAVDRFGIRRLYYAANEHGLAWSSRVRGALAIDSGGPRIDPD
                     AVYAYLNFGTIPAPQTIYAAARRLPPGHALIWEDGRVSLERYWDMSYPLRPTTESLAV
                     SAVRQQTREAVREALVGSDPKRTGAFLSGGTDSSTIVGLMKQVSDERVNAFSIGFAEE
                     RYDE"
BASE COUNT          305 a          770 c          793 g          356 t
ORIGIN      
        1 gggatcccgg tggtggtcac ctctcaggcg gcgcaggcgc tcggggccga ggcgggtcgg
       61 gacctcctgg tcgccgacgc cccccgtgag ttcgcctccc agatcctcca gctgctccgt
      121 gaccaggagg cggccgcgga aatgggcgcg cgcgggcagc tcctcgtcgc gaccaagtat
      181 gcctggagcg tgctcactgc cccggttcac gaactcgtcg agagcatcgc gaaggtgggt
      241 ccccctccgc cggcgccgaa gcccctggcc gggcttcgag ggtcgcgatg aagacccgcc
      301 gggtcgagtc gctcgctggg ttcgaagcct tgagccccgc gtgggccacg gtcgtacggg
      361 agagcgggca gacctcgccc ttcctgagcc acgaatggtt cacgtgctgc tggcgggggg
      421 cgggcgcggg tcggcggccg gaggcgatcc tcgtcgagga cacgaccgga ccggtcgggc
      481 tggtgccgct cacgcactgg aggggcaacc tgcatcgact gccggtgcgc ttcgtgggaa
      541 tgctgaattc cccggatacg ccgtatgtcg actggctcat ggtgggccgg cccgagcctg
      601 tcgtcgatgc ggttctcacc gagctgatgc ggcgtcgcga ctgggacgtg ttctcgctcg
      661 ccggccttcc ggccgactcg ctgaccctga aggcgctcga gacagttctc ccgggccggt
      721 tccgcacgct ccggctgccc gcgctaccgt ctccatacgt ggacgtgagc ggcagctgga
      781 acgccttctg gaccggtacc agccagcgct tcaagaagac ggtccgcagc atccggaacc
      841 gactccagaa ggcgggagag attcgcgtgg aggagcaccg cgacctcgct cccggcagcc
      901 cgatcctcga cgaggtgatc gacgtctccc gccggagctg gaaggcggtc gagaacgtgg
      961 cgatcgccaa catgccgggc atgagggatt tcttccggga gctgaccgcg tgcgcgagcc
     1021 ggaacggctg gctccgtctc tggctcctgc ggctggacgg gcgggtggtc gctaccgagt
     1081 accagctgga ggcggccggt cgtgttcacg cgctccgggc cgacttcgac ggcacgctgc
     1141 cgtcggacct ctcgcccggc actcacctga gcgaggaaat catccggcgg ctcttcgagc
     1201 ggcccggcat ccacgagtac gacatggggc cgggggacaa cgcctacaag cagcggtggg
     1261 ccaccggcac gcacgagcac gtgcggctcc tgatcttccg gcccggtgcc gtcggggcca
     1321 cgctgcacac gatggccaac cggatcgtcc ccgccctccg gcggctccgg cccggatctg
     1381 cccgatgagc ctcatcgcag gtctcgtgta ccgcgatccg gcgcgcccgg tcgaaccgga
     1441 ggttctggcg gccctcgtcg agggctcgtc cgtggtgcat ccgcgtgagt cccggctgga
     1501 cacgggcgcg gccttggtgg ccggcgggcc gggcgcttcg gccgaggcct gtggcccggt
     1561 cgtgctggcc gcggacatgg acgtgaccaa tgtgacggag ctgcttcgac agcgcggggt
     1621 cacgaccctt cccgagctta tgcacgacct ctacgagtcg gaaggtcggc gattcccgct
     1681 ccggctgcgg ggcggcttcg cgctggccct ctggtatccc gagcgtcggc ggctggtcct
     1741 cgcggtcgat cgcttcggca tccgccgtct ctactacgcg gccaacgagc acgggttggc
     1801 ctggagctcg cgggtgcggg gtgcgcttgc gatcgactcc ggcggtccgc ggatcgaccc
     1861 cgacgcggtg tacgcctatc tgaatttcgg gaccatcccc gccccgcaga ccatctacgc
     1921 ggcggcccgg cgcctgcctc ccggccatgc cctgatctgg gaggacggac gcgtgtctct
     1981 ggagcgctac tgggacatgt cctatccgct gcgtccgacc accgaatccc tggccgtgag
     2041 cgcggtacgc cagcagaccc gggaggcggt tcgcgaagcg ctggtcgggt ccgatcccaa
     2101 gcgtaccggc gccttcctga gcggcggaac cgacagcagc acgattgtgg gcctgatgaa
     2161 gcaggtctcc gacgagcggg tcaacgcctt ctcgatcggc ttcgccgagg agcggtacga
     2221 cgag
//
LOCUS       DATMCV010000884         3776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_181421, whole genome shotgun sequence.
ACCESSION   DATMCV010000884 DATMCV010000000
VERSION     DATMCV010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181421"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VNU03_20195"
     CDS             <1..1294
                     /locus_tag="VNU03_20195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76852.1"
                     /translation="DETIDGRGLMVMPGLIDVHSHPCLEASYRGIREEHGVPEMYMSG
                     LFERAGAYWPDDDGKLACAEAAYCELLRSGVTTLVDISGDYPGWIDLIARSGLRGVLA
                     PSYASSRWVVRRSHLLEYEWNEAAGRQGFEEALRLCDDLAKHPSGRLTGMISPAQIDT
                     CTEDLLRDSVAAARERGLPFTVHCAQAAGEFHEMVRRHGITPVQWAHQIGLGGPGTIL
                     GHAMFIDEHSWLHWWSRTDIAIMADAGTSVAHCPTPFARYGHTLEHFGKYRRAGVNLG
                     IGTDTVPQNLLEEMRWATVLARIAAEDIRATEMADVFHAATVGGAHALLREDLGRLAV
                     GARADLALVDCRNPWMMPVRDPLRSLIFHAADRAVRDVYVDGERVVADGRVLTLDQPG
                     ALDRLTAAQRRMEASVPARDARGRRSVDITPLSLPPMP"
     gene            1291..2553
                     /locus_tag="VNU03_20200"
     CDS             1291..2553
                     /locus_tag="VNU03_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWP76853.1"
                     /translation="MTAARRGLLERIEADRDRLVGFLQAFTRIDTANPPGDTRAGAAF
                     IGDFLERAGLPYRRIAPQETMPNLLAATAFARPGRHLVLNGHIDVFPAGDRARWTHDP
                     LSGAIVDGRLHGLGTVDMKCGTTASIFTYAYLSGLADELRGRLTLTLVSDEETGGRWG
                     SGYLVEHHRDEVLGDCVLNGEPSSPYTIRFGEKAMFWLKLSIRTPGGHSAYPHVSKSA
                     NRIAAALIRDLESLEALVPATPEKVARALGGPDVRAGAERGLGAGAAEFLEKVTLNVG
                     VMRGGVKVNMLPAACEIEADVRLPVGVDRDDVRREIERIVARYPEAAWEEGASQRANA
                     TWSDPEHAMVGHLQENVQAFRGFRPPAIISLGGTDCRFWRAAGTPAYVYGPSPAGMGA
                     PNESVDIEEFLHVVRTHTLSAFDYLSRA"
     gene            2550..2780
                     /locus_tag="VNU03_20205"
     CDS             2550..2780
                     /locus_tag="VNU03_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76854.1"
                     /translation="MITRRELERWLLREGATRVKRADGHKHFTLRGHHVVVLGHGPQT
                     LSATSVSLVMKQLEQAGYTRERLRREWSGAGA"
     gene            2839..>3776
                     /locus_tag="VNU03_20210"
     CDS             2839..>3776
                     /locus_tag="VNU03_20210"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HWP76855.1"
                     /translation="MRVSPDRLRRLTATIIARGGSEAAEADLVAGHLVDANLAGHDSH
                     GVSLIPHYVRHLQEGLVVPNTTAKVLKDDGAFLMFDGQRGFGRRVAGEAMAAAIARAR
                     ESGVAVMTLANSHHIGRVGAYGELASAAGLVSLHFVNVADHRGLVAPFRGSDSRFSTN
                     PVCIGMPGTATQPPLLLDMATSGVAMGKVLVAKLAGKRLPEGNLIDARGAPTTDPEVM
                     YQEPRGSLLPFGAHKGYALAVLTELLAGAMSGGGTIQPENPRRGGIVNNMFSIVVDPA
                     RLAGADWMRREIDGFVGYVKASPAADAAKPVLVPGDP"
BASE COUNT          520 a         1419 c         1336 g          501 t
ORIGIN      
        1 cgacgagacc atcgacgggc gcggcctcat ggtgatgccg ggcctcatcg acgtccactc
       61 ccacccctgc ctcgaggcca gctaccgcgg catccgggaa gagcacgggg tgcccgagat
      121 gtacatgtcg ggcctcttcg agcgcgcggg cgcctactgg ccggacgacg acggcaagct
      181 cgcctgcgcg gaggccgcct actgcgagct gctgcgctcc ggcgtcacca cgctggtcga
      241 catctcgggc gactacccgg gctggatcga cctcatcgcc cggagcgggc tgcgcggggt
      301 gctcgccccc tcctacgcat cgtctcgatg ggtggtgcgg cggtcccacc tcctcgagta
      361 cgagtggaac gaggcggccg gccgccaggg gttcgaggag gccctgcggc tctgcgacga
      421 cctcgccaag catccgagcg gccgcctcac cggcatgatc tccccggccc agatcgacac
      481 ctgcaccgag gatctgctgc gcgacagcgt ggccgccgca cgcgagcgcg ggctgccctt
      541 caccgtccac tgcgcccagg ccgcgggcga gttccacgag atggtgcgcc gccacgggat
      601 cacccccgtg cagtgggcgc accagatcgg cctcggcggg cccggcacca tcctcggcca
      661 cgccatgttc atcgacgagc actcgtggct gcactggtgg tcgcgcaccg acatcgcgat
      721 catggccgac gcggggacgt cggtcgcgca ctgcccgacc ccgttcgccc gctacggcca
      781 taccctcgag cacttcggca agtaccgccg ggccggggtg aacctcggca tcggcaccga
      841 caccgtgccc cagaacctgc tcgaggagat gcgctgggcc accgtgctcg cgcggatcgc
      901 ggcggaggac atccgcgcca ccgagatggc cgacgtcttc cacgccgcca ccgtgggcgg
      961 cgcccacgcg ctgctgcgcg aggacctcgg ccgcctcgcg gtgggggcca gggcggacct
     1021 cgccctggtg gactgccgga atccgtggat gatgccggtg cgcgatccgc tgcgctcgct
     1081 gatcttccac gccgccgacc gcgcggtgcg cgacgtctac gtcgacggcg agcgcgtggt
     1141 ggcggacggc cgcgtgctca ccctcgacca gccgggcgcc ctcgaccggc tcaccgccgc
     1201 gcagcggcgc atggaggcga gcgtgcccgc gcgcgacgcc cgcggccgcc gctccgtcga
     1261 catcaccccg ctcagcctgc ccccgatgcc atgaccgccg cgcgccgggg cctgctcgag
     1321 cggatcgagg ccgaccgcga tcggctcgtc ggcttcctgc aggccttcac ccgcatcgac
     1381 accgcgaacc cgccgggcga cacgcgcgcg ggcgccgcct tcatcggcga cttcctcgag
     1441 cgggccgggc tgccctaccg gcgcatcgcc ccgcaggaga cgatgcccaa cctgctcgcc
     1501 gcgacggcct tcgcccggcc gggccgccac ctggtgctca acggccacat cgacgtcttc
     1561 ccggccgggg atcgggcgcg ctggacgcac gacccgctct ctggcgcgat cgtggacggg
     1621 cgcctgcacg ggctcggcac ggtcgacatg aagtgcggca ccaccgcgtc gatcttcacc
     1681 tacgcctacc tgagcgggct cgcggacgag ctgcgcggcc gcctcaccct caccctggtc
     1741 tccgacgagg agaccggcgg acggtggggc agcggctacc tggtcgagca tcaccgggac
     1801 gaggtcctgg gcgactgcgt gctgaacggg gagccgagca gcccgtacac catccgcttc
     1861 ggcgagaagg cgatgttctg gctgaagctc tcgatccgca ccccgggcgg gcacagcgcg
     1921 tacccccacg tgagcaagag cgccaaccgg atcgcggccg cgctcatccg cgacctcgag
     1981 tcgctcgagg ccctcgtgcc cgccaccccc gagaaggtcg cgcgggccct cggtggcccc
     2041 gacgtccgcg cgggggccga gcgggggctg ggcgcgggcg cggccgaatt tctagagaag
     2101 gtgacgctca acgtcggggt catgcggggc ggggtgaagg tcaacatgct gcccgccgcg
     2161 tgcgagatcg aggcggacgt ccgcctgccg gtgggggtcg accgcgacga cgtgcggcgc
     2221 gagatcgagc gtatcgtcgc gcgctatccg gaggcggcgt gggaggaggg cgcgagccag
     2281 cgcgcgaacg cgacctggtc ggatcccgag cacgcgatgg tcggtcacct ccaggagaac
     2341 gtgcaggcgt tccgcgggtt ccggccgccc gccatcatct cgctcggcgg caccgactgc
     2401 cgcttctggc gcgcggccgg cacccccgcc tacgtgtacg gcccctcccc cgcgggcatg
     2461 ggcgccccga acgagtcggt cgatatcgag gagttcctcc acgtggtgcg cacccacacc
     2521 ctctccgcct tcgactacct gagtcgcgcg tgatcacgcg ccgagagctc gagcgctggc
     2581 tcctgcgcga gggcgccacc cgggtgaagc gggcggacgg ccacaagcac ttcacgctgc
     2641 ggggccatca cgtcgtggtg ctcggtcacg gcccccagac gctcagcgcc accagcgtgt
     2701 cgctcgtgat gaagcagctc gagcaggcgg gctacacgcg cgagcggctg cgccgggagt
     2761 ggagcggcgc gggcgcttga ttcacgcggc gtcgacgggt aggctgcgct ggtatccatc
     2821 ccccgccagg agatcccgat gcgcgtatcc cccgaccgtc ttcgccggct gaccgccacc
     2881 atcatcgccc gcggcggcag cgaggccgcc gaggccgacc tggtcgccgg tcatctggtc
     2941 gacgccaacc tcgccggtca cgacagccac ggggtgagcc tgatcccgca ctacgtgcgg
     3001 cacctccagg aaggcctcgt cgtgcccaac accaccgcga aggtgctgaa ggacgacggc
     3061 gcgttcctca tgttcgacgg ccagcgcggg ttcgggcgcc gggtggcggg cgaggcgatg
     3121 gccgctgcga tcgcgcgcgc ccgggagagc ggggtcgcgg tcatgaccct cgccaactcg
     3181 caccacatcg ggcgggtcgg ggcctacggc gagctggcca gcgcggccgg gctcgtctcc
     3241 ctccacttcg tcaacgtggc cgatcatcga gggctggtgg cgccgttccg ggggagcgac
     3301 tcgcgcttct cgaccaaccc ggtctgcatc ggcatgcccg gcaccgcgac ccagccgccg
     3361 ctgctcctcg acatggcgac cagcggcgtc gcgatgggca aggtgctggt ggcgaagctc
     3421 gccggcaagc gcctgcccga gggcaatctg atcgacgcgc ggggcgcgcc caccaccgat
     3481 cccgaggtga tgtaccagga gccgcgcggc tccctcctcc ccttcggagc gcacaagggc
     3541 tacgcgctcg cggtcctcac cgagctgctg gcgggggcga tgtccggggg cggcaccatc
     3601 cagcccgaga acccgcgccg cggcggcatc gtcaacaaca tgttcagcat cgtcgtcgat
     3661 ccggcccgcc tggccggggc ggactggatg cggcgggaga tcgacggctt cgttggctac
     3721 gtgaaggctt cgccggcggc ggacgccgcg aagccggtgc tggtgccggg cgatcc
//
LOCUS       DATMCV010000885        10423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_780622, whole genome shotgun sequence.
ACCESSION   DATMCV010000885 DATMCV010000000
VERSION     DATMCV010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10423
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780622"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VNU03_20215"
     CDS             <1..810
                     /locus_tag="VNU03_20215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HWP76856.1"
                     /translation="EKRFTFTPMASQHDLGLLQIPEEHGVLRVLDGQHRLLALHAVTQ
                     AGENLGIEVPAVLFDRLDARQIVELFVTINAKHTRLNPSHIVSLAGRKLYPDPNQAMA
                     HDVIRSLNEDETSPLAGEIKMLGTGRGRVSQAPLAEEIVDFLDAVEKIGGGARMNEVR
                     QHAKRFFLNYIKAVAGVFPTAWAGRKYSIKTGAALRAFIRVAPDVMARARELKRDAFD
                     FHAIREAVKPWGDRLKDRRFETEGEWKNKLAGGTRGTVEALTRELREALRG"
     gene            complement(943..1425)
                     /gene="greA"
                     /locus_tag="VNU03_20220"
     CDS             complement(943..1425)
                     /gene="greA"
                     /locus_tag="VNU03_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWP76857.1"
                     /translation="MSVQRTPMTRTGYERLRDEVTRLKKERPNIVRAIAEARAHGDLS
                     ENAEYHAARERQGFIEARITDLESKVGTAEVIDPPTSGDRVTFGSTVKLEDEDGKETI
                     YQIVGSDEADPKQGLISIMSPLARTLISKKVGDTAIATLPGGKKTFEILAANFPVNRA
                     "
     gene            complement(1422..1976)
                     /locus_tag="VNU03_20225"
     CDS             complement(1422..1976)
                     /locus_tag="VNU03_20225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HWP76858.1"
                     /translation="MDKTALLIIDAQQEYFAPIGKVVLPDGPKAVVRIADTLRWARES
                     RVPVFHIVHESRRPGATTFVPGSPALAIHDAVAPRAEEPIITKHLPGAFTDTPLEAEL
                     RRRGIERVIVSGFMTQMCCDTTSREAAHRGFKVTLLSDATAAMDVKGPDGVVIPHDQV
                     HRTHLGSLNGFLAEVKRSDEVIGT"
     gene            complement(1994..3895)
                     /locus_tag="VNU03_20230"
     CDS             complement(1994..3895)
                     /locus_tag="VNU03_20230"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HWP76859.1"
                     /translation="MMREYARSLKIVLLIVVVVFILTSGVLFYFGSGPFGSGKSNAVA
                     IVNGEEIPPERFKRAQANMMAAYARMARQQLTPELAERLGLSQQVINELVTEAIVIQG
                     AADEGVRVSDDELRVTIQQIREFQENGRFSRDEYLKVLRQIRLDPGEFETEVRRQLLR
                     RKMENLIKQGVKVSDEELRQAYRQRHEQVRAAWAYGDVKPAMAGVQVADADLEPYVKT
                     HQPQFSQPERRRLQYVLLAASPQAATVSDAEVDAYYKEHGSEFDEPKRLRIAHVLVRV
                     PPVGGSDAENAARAKVEDVIKRARAGEDFGKLAREISEDKASAVQGGDLGFVGPGELV
                     APFEQAAFALKKGDVSDPVRTPFGYHAIRILDVRDGGKTPLKDVAPKIKAALVAQKGE
                     RAAQARADEAKAALLGAPDFAAAAKRLGLEAREVTFGRGDALGEAGRDPQLDEAVFGL
                     AVGGVSTPLKTAAGYAVVKVAQQIPAGVPPLPEIRDRVVEAIKRERAEQQLTDKAKAL
                     AATLAKGSDFAAAAKAEGFTTGSIPLFSRSDPPKERGGLPGGVLLAALQTPAGQVAEP
                     VRAEGGVYVVKTLERQPASPGGFDRERDELDKQVLEQKRALAWDTWIRGRLASAKVEV
                     GGQPASMTR"
     gene            complement(4002..5387)
                     /locus_tag="VNU03_20235"
     CDS             complement(4002..5387)
                     /locus_tag="VNU03_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006132722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HWP76860.1"
                     /translation="MLARYFELGRLGTTPGGELRGGLTTFMVMAYIIFVNPAILSFAG
                     IPPLQGQGPSFAATQAATCLVAGVLTIAMGLVTNYPLALASGMGLNAVVAFQLVAGMK
                     LPWTAAMGVVLLEGLVITALVLTGFREAIMNAIPAALKRAIGVGIGLFILFIGLVSAG
                     IVKPGPPGVPVTLGDLSSAPVAVALFGLLLTLWLTARRSSGALLVGILASTVVAVAVN
                     AATGGHAFPTPGQAVWPIALVAWPDFSSLGAGLDLSVFARVGVVTAVVVIFSIMLSDF
                     FDTMGTVIGIGGEAGWLTRDGRLPRLDRVLLVDSLAAVAGGAAGASSATTYIESAAGV
                     AAGARSGLASVVTGLCFLLALFFAPLAGVVPAQATAPALIVVGYLMSRVVRDIPFGDF
                     EEGFPALLTLAVMPFTYSITNGIGAGFIAYCFIKLVRGKGGSVPVMMYGAALAFVIYF
                     VLPLIQRLLSA"
     gene            complement(5393..6226)
                     /locus_tag="VNU03_20240"
     CDS             complement(5393..6226)
                     /locus_tag="VNU03_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HWP76861.1"
                     /translation="MKSDKFEDGEQALLIDQRGRRHLLLLRKGETFHSDRGWVTHDAI
                     IGHPDGSWVRTSKGTRYLALRPTLAEYVLDMPRGAQVIYPKDLAMVMFWADIFPGARV
                     LEAGMGSGALTLALLRAVGPEGRVVSYEQREDFCRRALANIQMRLGEVTNLIVRIRPV
                     EDGLPEEEPVDRAVFDLPEPWRLVEPVASVLRPGGIFLSYVPTIIQSHQLTETLRRHR
                     GYALVETFETLLRPWNIDGTSVRPFHRMVAHTGFLTVARRVVPEEGAAATPRIADPET
                     D"
     gene            complement(6213..7442)
                     /locus_tag="VNU03_20245"
     CDS             complement(6213..7442)
                     /locus_tag="VNU03_20245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP76862.1"
                     /translation="MSPDPSSSSVALPSVGVLPAERARPDMRLLGLLALGHMVIDINQ
                     GSLPAILPIIKDALNLSYAATGVIVLSANVTSSMIQPLFGYLADKTARRWLLPLSVLL
                     SSVGLAFMGLAPSYAAVLALVMITGFGVAAYHPEGYRTATAVAGERKATGVSIFSTGG
                     NIGIALGPPLMTALLIGFGPAGSLGMLVPGLLVAGLLVAVLPRLSAPPPAAARARANA
                     SGARTMVGAMSLLILVVAIRSWTQLGFTTFMPFYWKDVLHGDPRLVGTLLAVFLGSGA
                     IGTLVAGPVADRVGIRRSVVGVFLLAAPLAGGFLLVSGGPLVFVLLALLGFVLVSTFT
                     MSVVLGQAYLPRSPGMASGLIVGFAIGAGGIGATGLGWVADHWGLTTALGISAAMPLA
                     GFVAALFLPDPQTGEVG"
     gene            complement(7472..8644)
                     /locus_tag="VNU03_20250"
     CDS             complement(7472..8644)
                     /locus_tag="VNU03_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP76863.1"
                     /translation="MEKFRGVDYYGIDDLLTEEQRMMRDAVRDWVEAKFLPVVSQHHR
                     DGTFPMELAKDLGEMGVFGATLKGYGCAGLDNVAYGLIMQELERGDSGLRSFASVQSG
                     LVMYPIHSYGSDAQKDRWLPRLQSGEALGCFGLTEPDHGSDPGSMKTRAVKKGNEYVL
                     NGTKLWITNGSVADVAVVWAKGDDGEIGGYLVEKGTPGYSTIDIHGKFSMRASITSEL
                     AFADCRIPLENKLPGVKGLKGPLSCLSQARYGIAWGAIGAAMGCYDWALQYSQQRIQF
                     NKPIGSFQLVQQKLVWMITEITKAQLLCWRLGQLKDAGRARPQQISMAKMNNVQMALD
                     GARLARDILGAAGIVDEHPIIRHMMNLETVNTYEGTHDIHTLIVGRDITGLDAFGI"
     gene            complement(8666..10069)
                     /locus_tag="VNU03_20255"
     CDS             complement(8666..10069)
                     /locus_tag="VNU03_20255"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWP76864.1"
                     /translation="MKGVDTYQGRGLIADPIHQYILYTRPEGTPGESTEQDLIDSPWM
                     QRLRRVPQLQSARWVFPAAEHSRFQHSLGAMHLAGRFALQLHRSLQAEFADAPSAPLF
                     EELMRVAGLLHDVGHGPFGHFFDDNFLADFDLTHEKVGQRIIREELGDLIRGLRRSPS
                     GPFEDGERIDPEWICYLMGKSYTVPTAEHPRWLAHLKPLLSGIYTADNMDYVLRDSYM
                     CGVAVGPIDIERIMYYSFFSEKGLTLDRGGIQAFIMFLNARFYLYTNVYYHRTTRGID
                     LHLKEIFRETMKLVFPFDLNKELQPYLNMTEWTLLEEVAHWDHSDEPDRRALGREWRQ
                     VLDRHLKWRMSHEVVLDLFEPRRGQSFMKAEEVEQRVRELLPPGLHEFPFKIDMAQQD
                     PRPLNPIGMQDRQIYIYDAASRTVSAEPLKELLKYLPGKVAQCRIFAMSHEHDQRLAA
                     ALEQALGEDRPAHPTNL"
     gene            complement(10101..>10423)
                     /locus_tag="VNU03_20260"
     CDS             complement(10101..>10423)
                     /locus_tag="VNU03_20260"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWP76865.1"
                     /translation="ELIARGRVTSRPPRPWLGLYTEPGAGGLLVSGLSPLSPARAAGL
                     RPGDVIVQLNGRQVASREDFYRTLWQAEVDRDVHVTVARPGGHHAITVRPVDRYRFYR
                     TSDQ"
BASE COUNT         1599 a         3622 c         3683 g         1519 t
ORIGIN      
        1 gagaagcgct tcaccttcac cccgatggcg agccagcacg acctgggtct gctccagatt
       61 ccggaggagc acggtgtcct gcgcgtgctc gacggccagc accggctgct cgccctgcac
      121 gcggtcaccc aggcgggcga gaaccttggc atcgaggtcc cggcggtgct cttcgatcgg
      181 ctggacgcgc gccagatcgt ggagctgttc gtcaccatca acgccaagca cacgcgactc
      241 aacccgtccc acatcgtgag cctggccggg cgcaagttgt atccggatcc caaccaggcg
      301 atggcgcacg acgtgatccg ctcgctcaac gaggacgaga cctcgccgct cgcaggcgag
      361 atcaagatgc tcggcaccgg gcgggggcgc gtctcgcagg ccccgctcgc cgaggagatc
      421 gtggacttcc tcgacgcggt ggagaagatc ggcggcgggg cccgcatgaa cgaggtgcgc
      481 cagcacgcca agcggttctt cctgaactac atcaaggcgg tggcgggggt cttccccacc
      541 gcgtgggcgg ggcggaagta ttccatcaag accggcgccg cgctgcgcgc cttcatccgc
      601 gtcgctcccg acgtgatggc ccgggcccgc gagctcaagc gcgacgcgtt cgatttccac
      661 gccatccgcg aggcggtcaa gccgtggggc gaccgactga aggaccgccg cttcgagacc
      721 gagggcgagt ggaagaacaa gctcgcgggc ggcacccgcg gcaccgtcga agccctcacc
      781 cgcgagctgc gcgaggcgct gcgggggtag ctgggcgggg cgggcgggca agcgtttcga
      841 cgctgccgcc acgcgctgcg cttcgagcta ccggagcttc tcttcatctg cgggggccca
      901 gaaatggccc ccgcactccc ccagttttcc tgtctttccg ctctaggcgc gattcactgg
      961 gaagttcgcg gcgaggatct cgaaggtttt cttgccgccc gggagggtcg cgatggcagt
     1021 gtcgccgacc ttcttgctga tcagggtgcg ggccagcggg gacatgatcg agatcagccc
     1081 ctgcttcggg tccgcctcgt ccgagcccac gatctggtag atcgtctcct tgccgtcctc
     1141 gtcctcgagc ttcacggtgg agccgaaggt cacccggtcg cccgacgtcg gcggatcgat
     1201 cacctcggcg gtgccgacct tggactcgag atcggtgatg cgcgcctcga tgaacccctg
     1261 gcgctcccgc gccgcgtggt actccgcgtt ctcgctcagg tcgccgtggg cccgcgcctc
     1321 cgcgatggcc cgcacgatat tcgggcgctc cttcttgagc cgagtcacct cgtcgcgcag
     1381 ccgctcgtag ccagtgcgcg tcatcggggt gcgctggacg ctcatgtccc gatcacctcg
     1441 tcgctgcgct tgacctcggc caggaagccg ttgaggctcc ccaggtgcgt gcggtggacc
     1501 tggtcgtggg ggatcaccac gccgtcgggc cccttgacgt ccatcgcggc ggtggcgtcc
     1561 gagagcagcg tcaccttgaa gccgcggtgg gccgcctcgc ggctggtggt gtcgcagcac
     1621 atctgggtca tgaagccgga gacgatgacc cgctcgatcc cgcgacggcg gagctcggcc
     1681 tcgagcggcg tgtcggtgaa cgcgccgggc aagtgcttgg tgatgatcgg ctcctccgcc
     1741 cgcggcgcca ccgcgtcgtg aatggcgagc gcggggctcc ccggcacgaa ggtggtggcc
     1801 ccgggccggc ggctctcgtg gacgatgtgg aagacgggca cccggctctc gcgcgcccag
     1861 cgcagggtgt ccgcgatgcg gaccaccgcc ttggggccat caggcaggac caccttgccg
     1921 atcggggcaa agtactcctg ctgggcgtcg atgatgagga gcgcggtctt gtccatcgtg
     1981 agcctcccgg cggtcagcgc gtcatggagg cgggctggcc gcccacttcg accttggccg
     2041 acgcgagccg gccgcggatc caggtgtccc acgcgagcgc ccgcttctgc tcgagcacct
     2101 gcttgtccag ctcgtctcgc tcccggtcga agccgccggg gctggccggc tgccgctcca
     2161 gcgtcttgac cacgtagacg ccgccctcgg cgcgcaccgg ctccgcgacc tgcccggccg
     2221 gggtctgcag cgcggccagg agcacgccgc cgggaaggcc gccgcgctcc ttcggcggat
     2281 ccgagcggga gaacagcggg atgctcccgg tggtgaatcc ctccgccttg gccgccgccg
     2341 cgaagtcgct gcccttggcg agggtcgcgg ccagcgcctt ggccttgtcg gtcagctgct
     2401 gctcggcccg ctcgcgcttg atggcctcga cgacgcggtc gcggatctcg ggcagcggcg
     2461 gcacccccgc cgggatctgc tgcgccacct tgaccaccgc gtagccggcc gcggtcttga
     2521 gcggcgtgga gacgccgccc accgcgagcc cgaataccgc ctcgtcgagc tgcggatcgc
     2581 ggccggcctc gccgagcgcg tcgccgcgcc cgaaggtgac ctcgcgcgcc tcgagcccga
     2641 ggcgcttggc cgccgccgcg aagtcggggg cgccgagcag ggcggccttc gcctcgtccg
     2701 cgcgggcctg ggccgcgcgc tcgcccttct gcgcgacgag ggccgccttg atcttcgggg
     2761 ccacgtcctt gagcggcgtc ttgcccccgt cccgcacgtc gaggatccgg atcgcgtggt
     2821 agccgaaggg cgtgcgcacc ggatcggaga cgtcgccctt cttgagggcg aaggcggctt
     2881 gctcgaacgg ggccaccagc tccccagggc ccacgaaacc gaggtcgcct ccctgcaccg
     2941 cgctcgcctt gtcctccgag atctcccgcg cgagctttcc gaagtcctcc cccgccctcg
     3001 cgcgcttgat cacgtcctcg accttcgccc tggccgcgtt ctccgcgtcg ctgcccccca
     3061 ccggaggcac ccgcaccagc acgtgggcga tccggaggcg cttcggctcg tcgaactcgg
     3121 acccgtgctc cttgtagtaa gcgtcgacct ccgcgtccga gaccgtggcg gcctgcgggc
     3181 tcgcggcgag cagcacgtac tggagccgcc gccgctcggg ctggctgaac tgcggctggt
     3241 gggtcttgac gtacggctcg aggtcggcgt ccgccacctg cactccggcc atcgcgggct
     3301 tgacgtcgcc gtaggcccac gcggcgcgca cctgctcgtg gcgctggcgg taggcctgcc
     3361 gcagctcctc gtcagagacc ttcacgccct gcttgatcag gttctccatc ttgcgccgga
     3421 ggagctggcg ccgcacctcg gtctcgaact cgccgggatc gagccggatc tgtcgcagca
     3481 ccttcaggta ctcgtcgcga gagaaccgtc cgttctcctg gaactcgcgg atctgctgaa
     3541 tggtgacccg cagctcgtcg tcgctgacgc gcacgccctc gtcggccgcc ccctggatca
     3601 cgatggcctc ggtcaccagc tcgttgatca cctgctgcga caggccgagc cgctcggcca
     3661 gctccggggt cagctgctgg cgcgccatgc gagcgtaggc cgccatcatg ttggcctggg
     3721 cgcgcttgaa gcgctcgggc gggatctcct cgccgttgac gatcgcgacc gcgttgctct
     3781 tgccgctgcc gaaggggccg gacccgaaat agaagaggac gcccgaggtg aggatgaaga
     3841 ccacgacgac gatcaggagc acgatcttca ggctcctggc gtattcgcgc atcatgctga
     3901 tcacggcggc ggcctcctag cgggaacgcc cggtcgacac gttcgagggc ttggcaatca
     3961 aggccttagc gtaccacacg acgggacgcg cgccggacgg cctaggcgga gagcaggcgc
     4021 tggatcagcg gcagcacgaa gtagatcacg aaggcgagcg cggccccgta catcatgacc
     4081 ggcaccgagc cgcccttgcc gcgaaccagc ttgatgaagc agtaggcgat gaagccggcg
     4141 ccgatgccgt tggtgatcga gtaggtgaac ggcatcaccg ccagggtgag gagcgcgggg
     4201 aagccctcct cgaaatctcc gaaggggatg tcgcggacca cgcggctcat caggtagccc
     4261 accacgatca gggccggcgc ggtggcctgg gccggcacca cgcccgcgag cggggcgaag
     4321 aacagcgcga gcaggaaaca gaggccggtc accaccgagg cgagcccgct gcgtgccccc
     4381 gccgccacgc ccgccgccga ctcgatgtag gtggtggccg acgaggctcc cgccgcgccg
     4441 cccgccaccg cggccagcga gtccaccagg agcacccggt cgagccgcgg cagccgcccg
     4501 tcccgggtga gccagcccgc ctcgccgccg atgccgatca cggtgcccat cgtgtcgaag
     4561 aagtcggaga gcatgatcga gaagatgacc accaccgcgg tgacgacgcc cacccgcgcg
     4621 aagacggaca ggtcgagccc ggcgcccagg ctcgagaagt ccggccacgc caccagcgcg
     4681 atcggccaca ccgcctgccc cggggtcggg aacgcgtgcc caccggtggc cgcgttcacc
     4741 gccaccgcca ccacggtgct cgcgaggatg ccgaccagga gcgcgccgct cgagcgccgc
     4801 gcggtgagcc acagggtgag caggagaccg aacagggcca ccgccaccgg cgccgacgaa
     4861 agatcgccga gggtcaccgg caccccgggc ggaccgggct tcacgatgcc cgccgacacg
     4921 aggccgatga agaggatgaa gaggccgatg ccgacgccga tggcgcgctt gagcgcggcc
     4981 gggatcgcgt tcatgatcgc ctcgcgaaag ccggtcagca ccagcgcggt gatcaccagg
     5041 ccttccagca gcaccactcc catcgcggcg gtccacggca gcttcatgcc cgccacgagc
     5101 tggaaggcga ccaccgcgtt gagccccatg ccggaggcga gggcgagcgg gtagttggtg
     5161 acgagcccca tcgcgatggt gagcacgccg gccacgaggc aggtggcggc ctgggtggcc
     5221 gcgaacgacg ggccctgccc ctgcagcggc gggatgcccg cgaaggagag gatcgcggga
     5281 ttcacgaaga tgatgtaagc catcaccatg aaggtggtga ggccgccgcg gagctcgccc
     5341 cccggggtcg tgccgagccg gcccagctcg aagtagcgcg cgagcacgcg ggtcagtcgg
     5401 tctcgggatc ggcgatgcgg ggcgtcgcgg ccgcgccttc ttccggcacc acccggcggg
     5461 ccaccgtgag gaagccggtg tgggccacca tccggtggaa cgggcgcacc gaggtgccgt
     5521 cgatgttcca cgggcggagc agcgtctcga acgtctccac gagggcgtag ccgcggtggc
     5581 gccgcagggt ctcggtgagc tggtgcgact ggatgatcgt cggcacgtag gagaggaaga
     5641 tgccgcccgg ccgcagcacg ctcgccaccg gctccaccag acgccagggc tccggcagat
     5701 cgaacaccgc ccgatccacc ggctcctcct cggggagccc gtcctccacc gggcggatgc
     5761 gcacgatcag gttggtcacc tcgccgagcc gcatctggat gttggcgagc gcgcggcggc
     5821 agaagtcctc gcgctgctcg tagctgacca cccggccctc gggtccgacc gcgcgcagga
     5881 gcgcgagggt gagcgcgccc gagcccatcc ccgcctcgag cacgcgcgcg cccgggaaga
     5941 tgtcggccca gaacatcacc atcgccagat ccttgggata gatgacctgg gccccgcgcg
     6001 gcatgtccag cacgtactcg gcgagggtcg gccgcagggc gagatagcgc gtgcccttgg
     6061 aggtgcggac ccaggagccg tcggggtgcc cgatgatcgc gtcgtgggtc acccagccgc
     6121 gatcggagtg gaaggtctcg cccttgcgca gcaggagcag atggcggcga ccccgctgat
     6181 cgatcagcag ggcctgctcg ccgtcctcga acttatccga cttcaccggt ctggggatcc
     6241 ggcaggaaca gggcggccac gaagccggcc agcggcatgg cggccgagat accgagcgcg
     6301 gtggtgaggc cccagtggtc ggcgacccat ccgagcccgg tcgcgccgat cccacccgcg
     6361 ccgatggcga agcccacgat cagccccgag gccatgccgg ggctccgcgg caggtaggcc
     6421 tggccgagca cgaccgacat cgtgaaggtg gagacgagca cgaagccgag cagggcgagc
     6481 aggacgaaca ccagcggccc gccggatacc aggaggaacc cgcccgcgag cggcgcggcg
     6541 aggaggaaca cgcccaccac ggagcggcgg atcccgacgc gatcggccac cggccccgcg
     6601 accagcgtgc cgatggcgcc cgagcccagg aacaccgcga gcagggtgcc gaccaggcgc
     6661 ggatcgccgt ggagcacatc cttccagtag aacggcatga aggtcgtgaa gccgagctgg
     6721 gtccaggagc ggatcgcgac caccaggatc agcaggctca tcgcgcccac catggtgcgg
     6781 gcgcccgacg cgttggcgcg ggcgcgcgcg gcggccggcg gcggcgcgga gagccggggc
     6841 agcaccgcga cgagcaggcc cgccaccagg aggccgggca cgagcatccc gaggctgccc
     6901 gccggcccga agccgatgag cagcgcggtc atcagcggtg ggcccagcgc gatgccgatg
     6961 ttgcccccgg tggagaagat ggagacgccg gtggccttgc gctctcccgc caccgcggtg
     7021 gcggtgcgat atccctccgg atggtaggcg gcgaccccga acccggtgat catgaccagc
     7081 gcgagcaccg ccgcgtacga gggcgcgagc cccatgaagg cgagccccac cgaggagagc
     7141 agcaccgaga gcgggagcag ccagcggcga gccgtcttgt cggccaggta gccgaagagc
     7201 ggctggatca tcgacgacgt gacgttggcg ctcagcacga tcactccggt cgcggcgtag
     7261 gacaggttga gcgcgtcctt gatgatcggg aggatcgcgg gcagcgagcc ctggttgatg
     7321 tcgatgacca tgtgcccgag ggccaggagg ccgagcaggc gcatgtcggg gcgggcgcgc
     7381 tccgcgggca agacgccgac ggacggcagg gcgacgctgg acgaggacgg gtccgggctc
     7441 atgggctccc tatgctacac cagccgcgcg atcagatgcc gaaggcgtcc agccccgtga
     7501 tgtcccgccc gacgatcaag gtgtggatgt cgtgcgtccc ttcataggta ttcaccgttt
     7561 caagattcat catgtgccgg atgatcggat gctcgtcgac gatgccggcc gcgcccagga
     7621 tgtcgcgggc caggcgcgcg ccgtcgagcg ccatctgcac gttgttcatc ttggccatcg
     7681 agatctgctg cggccgcgcc cgccccgcgt ccttcagctg gccgagccgc cagcagagga
     7741 gctgcgcctt ggtgatctcg gtgatcatcc acaccagctt ctgctgcacg agctggaagg
     7801 agccgatcgg cttgttgaac tggatgcgct gctgcgagta ctggagcgcc cagtcgtagc
     7861 agcccatcgc ggccccgatc gcgccccacg cgatgccgta gcgggcctgg gacaggcagg
     7921 agagcgggcc cttgagcccc ttcaccccgg gcagcttgtt ctcgagcggg atgcggcagt
     7981 ccgcgaacgc gagctcggag gtgatcgagg cgcgcatgga gaacttgccg tggatgtcga
     8041 tcgtgctgta gcccggcgtc cccttctcca ccaggtagcc gccgatctcg ccgtcgtcgc
     8101 ccttggccca caccaccgcc acgtccgcca ccgagccgtt ggtgatccag agcttggtgc
     8161 cgttcagcac gtactcgttc cccttcttga ccgcccgcgt cttcatcgag ccgggatcgg
     8221 agccgtggtc gggctcggtg aggccgaagc agcccagggc ctcgccggac tgcaggcggg
     8281 ggagccagcg gtccttctgg gcgtcggagc cgtagctgtg gatcgggtac atgacgagcc
     8341 cgctctgcac cgaggcgaac gagcgcaggc ccgagtcgcc ccgctccagc tcctgcatga
     8401 tgagcccgta ggccacgttg tcgaggcccg cgcagccgta gcccttgagg gtggccccga
     8461 agacccccat ctcgccgaga tccttcgcga gctccatcgg gaacgtgccg tcccggtgat
     8521 gctggctcac gacgggaagg aacttcgcct ccacccagtc gcggaccgcg tcccgcatca
     8581 tgcgctgctc ttcggtcagg agatcgtcga tgccgtagta gtccacgccg cgaaactttt
     8641 ccatgacgcc tgtccttcga ctcggttaga ggttggtggg gtgggcgggc cggtcttccc
     8701 cgagggcctg ctcgagcgcg gcggcgagcc gctggtcgtg ctcgtggctc atggcgaaga
     8761 tccggcactg ggcgaccttg ccgggcaggt acttcagcag ctccttgagc ggctccgccg
     8821 acacggtgcg cgaggccgcg tcgtagatgt agatctggcg gtcctgcatg ccgatcgggt
     8881 tgagcgggcg cgggtcctgc tgggccatgt cgatcttgaa gggaaactcg tgcaggccgg
     8941 gtggcagcag ctcgcgcacc cgctgctcga cctcctccgc cttcatgaag ctctgcccgc
     9001 gccgcggctc gaagaggtcg agcaccacct cgtgggacat gcgccacttg aggtggcggt
     9061 cgagcacctg gcgccactcg cggccgaggg cccggcggtc cggttcgtcg gagtggtccc
     9121 agtgggcgac ctcctccagc agggtccact cggtcatgtt gaggtagggc tggagctcct
     9181 tgttcaggtc gaagggaaag accagcttca tggtctcccg gaagatctcc ttcaggtgga
     9241 ggtcgatgcc gcgggtggtg cggtgatagt agacgttggt gtagaggtag aagcgggcgt
     9301 tcaggaacat gatgaacgcc tggatcccac cgcgatccag ggtgagcccc ttctcgctga
     9361 agaaggagta gtacatgatc cgctcgatgt cgatcggccc gaccgcgacc ccgcacatgt
     9421 aggagtcgcg cagcacgtag tccatgttgt cggcggtgta gatgccggag agcagcggct
     9481 tcaggtgcgc gagccagcgc gggtgctcgg cggtgggcac ggtatagctc ttgcccatca
     9541 agtagcagat ccactccggg tcgatgcgct ccccgtcctc gaagggcccg ctggggctgc
     9601 gccgcaggcc gcggatgagg tcgcccagct cctctcgaat gatacgttgg ccgactttct
     9661 catgggtgag atcgaagtcg gcgaggaagt tgtcgtcgaa gaagtggccg aagggcccgt
     9721 ggcccacgtc gtggagcagc cccgccaccc gcatcagctc ctcgaagagc ggcgcggagg
     9781 gcgcgtcggc gaactcggcc tggagcgacc ggtggagctg gagcgcgaag cgccccgcga
     9841 ggtgcatcgc ccccagggag tgctggaagc ggctgtgctc ggcggccggg aacacccacc
     9901 gcgccgactg cagctgcggc acccggcgca gccgctgcat ccacggggag tcgatcagat
     9961 cctgctcggt cgactcgccg ggcgtcccct ccgggcgggt atagaggatg tactggtgga
    10021 tcggatcggc gatgagaccg cggccctggt aggtgtcgac acccttcatg gctaagagcc
    10081 taacctagtt ggagcccaac ttactggtcg gaagtgcgat agaaacggta gcgatccacc
    10141 gggcgcaccg tgatcgcgtg gtggcctccc ggccgcgcca ccgtcacgtg gacgtcgcga
    10201 tccacttccg cctgccagag cgtgcggtag aagtcctcgc ggctcgcgac ctggcggccg
    10261 ttcagctgga cgatgacgtc gcccggccgc aaccccgccg cgcgggccgg gctcagcggc
    10321 gagaggcccg agaccagcag cccgcccgcc ccaggctcgg tgtagagccc gagccagggg
    10381 cgcggcgggc ggctcgtgac gcggccccgc gcgatcagct cgt
//
LOCUS       DATMCV010000886         5640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_711764, whole genome shotgun sequence.
ACCESSION   DATMCV010000886 DATMCV010000000
VERSION     DATMCV010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711764"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VNU03_20265"
     CDS             <1..226
                     /locus_tag="VNU03_20265"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422796.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWP76866.1"
                     /translation="LPAPEAAHAVRGAIDEALMAKKEGKRKTIVFNLSGHGLLDLSAY
                     EAYLAGKLQDYEYPSEKVAEALKHLPKVKG"
     gene            232..1230
                     /locus_tag="VNU03_20270"
     CDS             232..1230
                     /locus_tag="VNU03_20270"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76867.1"
                     /translation="MLGRRELLKLAGAGAAAAMTGAPAVLRAQAKGKVRLAYLQLGWA
                     GTEIIHKEDLLGRHGWSAEYSVVPGAPGPLLSQLAAKNVDAIDMSFALAAKAFEEGVP
                     LKVTGVATAVLGAIVARKDAGLSRVEDLKGRKIAAVVGTSTFFDIRALTLKGYGLDLQ
                     KDTQLITATAPPDMVTMLGKKDVDAIVAWQPITDQVVFRGEGVYLAKQIDLWRKATGR
                     ATGFPVHVCYLASPDFIQKNPSFPADLNAAQKDAVDLWYGKPERAIDIVTEVTKLPKD
                     VIAVAYKETVRMLSGLPEEQIETLIVQLKLNKEFGFLKSDLWDNPDRIRREFFHRA"
     gene            1227..1985
                     /locus_tag="VNU03_20275"
     CDS             1227..1985
                     /locus_tag="VNU03_20275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76868.1"
                     /translation="MSARDWAGRLHLPTLAALVVVLAGWTLLARRYGAFVLPSPWSIV
                     TGFVEIVRSGEIWGHAGASLGRIAAGFGSAVAVSLLMGLGAFVSRTARGIVQDVLAIL
                     NSTSVFVWIVVSLVWFGLSNWAPIFTTFMITLPVVASTLVEGVASVDRRLLEMGDVYR
                     LSGPQKFTAIVIPSTLPYLVAGMKVGFGLALKVSVVAEIFGVTSGIGYAMNYSREILA
                     TRMVFVWALVMILIMVATDKLLFDPVNRRLTRWR"
     gene            1976..2725
                     /locus_tag="VNU03_20280"
     CDS             1976..2725
                     /locus_tag="VNU03_20280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP76869.1"
                     /translation="MALKLEVRGIGKDFLIPVIDSVSFRVEAGEFVCLLGPNGCGKTT
                     LLRIVGGIEPASRGAVLLDGLPVQADRRHQRHVGIVFQEDRLLPWLSLRDNVTLVLRP
                     LGLDAAEREARAARYLKLAGLQGFEDYYPGRVSGGMRQRAAIARALAIEPDVLLMDEP
                     FGALDAQNRRIMQQEVRRIWSETRRTILFVTHSIEEAVAIGTTLIMLSARPARIRELI
                     SNDGRHERGKLVDDLNAMIMEEVQRQQGDAS"
     gene            2722..3789
                     /locus_tag="VNU03_20285"
     CDS             2722..3789
                     /locus_tag="VNU03_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005256270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWP76870.1"
                     /translation="MIKPYTAVGLIPTVRGIRKRKDIQINLDHLSHLVKAASWLSSLD
                     LPVRLIAFPEGALQAFNDEVLDLDHATFARECAIDIPGEETEALGKIAKEYNAFVMAQ
                     AKARHPDWKDRYFNVGFIINPRGRVILQHYKVSPLFPVEHSMCPHDVYDWWVEKYGRT
                     LQSFWPVVDTEIGRLGIMMANEGSYPENARALALNGAEVVYRASYPHPATGNEIFEIQ
                     SRARALDNNMYLVAPNMGTYHLFPEESTPIDTFGGRSFVIDYKGRIVGRQDYGAGSTY
                     VGGVIDVEALRDHRARAQWDNWMKDLRTELYGMLYEKPIYPKNLYLKRAPMKHKEYRE
                     KIIRRQIRLMHDRGIWAKPAR"
     gene            3813..5012
                     /locus_tag="VNU03_20290"
     CDS             3813..5012
                     /locus_tag="VNU03_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP76871.1"
                     /translation="MSNTSPTALLLNVGHAMDHLFLLIFATAVSAIAAEWGMVWQDLM
                     PYTVGAFVLFGLGSLPAGRLGDLWGRRAMMVIFFLGLGATGLLVAAAQRPWQLAMALT
                     VMGAFAAIYHPVGIPMIVQNSTRPGFTIGLNGLAGNLGIAVAAVLTGFLVQRVGWRMA
                     FMVPALLAIASGVLFALVVPREEMAPARKPRRSVDLPASIMMRIFFVMTLAAVSGSMI
                     FNFTTNGNNQLLAERLRGLVEDPAKLGVLLALVYTVASFAQIVVGRLIDRYPLKTVYL
                     PIVAAQVPLFLLAANAQGWVFYAIAVVFMVFVFGAIPFIDAMIVQYVDDRMRSRVAGM
                     RLAVSFGVSALAVYLLGPTVKAAGFTNLLLIMAGISASTTLFVSLLPGRIPTAAAASA
                     SAPAPAR"
     gene            5033..5518
                     /locus_tag="VNU03_20295"
     CDS             5033..5518
                     /locus_tag="VNU03_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP76872.1"
                     /translation="MPDPAFSPEEHRLAAPRWFDDLTVGERFPIPSRTMTEALFAAFQ
                     LASADNHPIHYDREYCRAHGHRDLLAHGLLVTAQAAAGAGIFPHAISDSLVAFLDQSS
                     RFLKPVYVGDTLYPLLTITRLEPGRTTGVVAMRATVHNQARELVMEGEHRYLLRRKPP
                     A"
BASE COUNT          835 a         2079 c         1824 g          902 t
ORIGIN      
        1 cctgcccgcc ccggaggcgg cccacgcggt gcggggcgcg atcgacgagg cgctcatggc
       61 caagaaggaa ggcaagcgga agacgatcgt gttcaacctc tctggccacg gcttgctcga
      121 cctctccgcc tacgaggcct acctggccgg caagctccag gattacgagt accccagcga
      181 gaaggtcgcg gaggccctca agcacctgcc gaaggtgaag ggctagccgc ggtgctcggc
      241 cgccgcgagc tgctgaagct ggccggcgcg ggcgcggccg ccgcgatgac cggcgcgccc
      301 gccgtcctgc gcgcccaggc caagggcaag gtccgcctgg cctacctcca gctcggctgg
      361 gccggcaccg agatcatcca caaggaggac ctgctcggcc gtcacggctg gagcgcggag
      421 tattccgtcg tccccggcgc gcccggcccg ctgctgagcc agctcgcggc caagaacgtc
      481 gacgccatcg acatgagctt cgcgctcgcc gccaaggcct tcgaggaggg cgtgccgctc
      541 aaggtgaccg gggtggccac cgcggtgctc ggggccatcg tggcccgcaa ggacgccggg
      601 ctcagccgag tggaggacct gaagggccgc aagatcgcgg cggtggtcgg cacctccacc
      661 ttcttcgaca tccgggcgct caccctcaag ggctacggcc tcgacttgca gaaggatacc
      721 cagctcatca ccgccaccgc gcccccggac atggtcacca tgctgggcaa gaaggacgtg
      781 gacgccatcg tggcgtggca gcccatcacc gaccaggtgg tgttccgcgg cgagggcgtc
      841 tacctggcca agcagatcga tctctggcgg aaggccaccg ggcgcgccac cggcttcccg
      901 gtccacgtct gctacctggc gagccccgac ttcatccaga agaacccgtc cttcccggcg
      961 gacctgaatg cggcgcagaa ggacgcggtc gacctctggt acggcaagcc ggagcgggcc
     1021 atcgacatcg tcaccgaggt caccaagctc ccgaaggacg tgatcgcggt cgcctacaag
     1081 gagacggtgc gcatgctctc gggcctgccc gaggagcaga tcgagaccct gatcgtccag
     1141 ctcaagctga acaaggagtt cggcttcctc aagtccgacc tctgggacaa ccccgaccgc
     1201 atccgtcgcg agttcttcca tcgggcgtga gcgcccgcga ctgggccggc cgcctgcacc
     1261 tgccgaccct cgccgctctc gtcgtggtcc tggccggctg gaccctgctg gcccgccgtt
     1321 acggggcctt cgtgctcccc tccccgtggt cgatcgtgac cggcttcgtc gagatcgtgc
     1381 ggagcgggga gatctggggc cacgccggcg cctcgctcgg ccgcatcgcg gccggcttcg
     1441 ggagcgcggt ggcggtctcg ctcctcatgg gcctgggcgc gttcgtgtcg cgcaccgcgc
     1501 gcggcatcgt gcaggacgtg ctcgccatcc tcaactccac ctcggtcttc gtgtggatcg
     1561 tcgtctcgct cgtctggttc gggctcagca actgggcccc gatcttcacc accttcatga
     1621 tcaccctgcc ggtggtggcc tccacgctgg tggaaggggt ggcctcggtc gaccgccggc
     1681 tcctcgagat gggcgacgtc taccggctca gcgggccgca gaagttcacc gcgatcgtga
     1741 tcccgtcgac cctgccctac ctggtggcgg gcatgaaggt gggcttcggc ctggccctca
     1801 aggtctcggt ggtcgcggag atcttcggag tcacctcggg gatcggctac gcgatgaact
     1861 acagccgcga gatcctggcc acccgcatgg tcttcgtctg ggccctggtg atgatcctca
     1921 tcatggtcgc caccgacaag ctgctgttcg atccggtcaa ccggcggctc acccgatggc
     1981 gctgaagctc gaggtgcgcg ggatcgggaa ggacttcctc atcccggtga tcgactcggt
     2041 ctcgttccgg gtggaggcgg gcgagttcgt ctgcctgctc ggaccgaacg gctgcggaaa
     2101 aaccaccctt ctccgaatag tcggcggcat cgagccggcc agccggggcg cggtgctcct
     2161 cgacggcctc ccggtgcagg ccgatcggcg ccaccagcgg cacgtgggga tcgtcttcca
     2221 ggaggatcgc ctcctgccct ggctctcgct gcgcgacaac gtgaccctgg tgctccgccc
     2281 cctcgggctc gacgcggcgg agcgggaggc ccgggccgcg cgctacctga agctggccgg
     2341 gctgcagggc ttcgaggact actatccggg ccgggtcagc gggggcatgc gccagcgcgc
     2401 ggccatcgcc cgggcgctcg ccatcgagcc cgacgtgctc ctgatggacg agccgttcgg
     2461 ggccctcgac gcccagaacc ggcgcatcat gcagcaggaa gtccggcgca tctggagcga
     2521 gacccggcgt accatcctct tcgtcaccca ctccatcgag gaggcggtcg ccatcgggac
     2581 caccctgatc atgctgtcgg cgcggcccgc gcgcatccgc gagctgatct cgaacgacgg
     2641 ccgccacgag cgggggaagc tggtcgacga tctcaacgcg atgatcatgg aagaggtgca
     2701 gcgccaacaa ggagacgcgt catgatcaag ccctacaccg cggtcgggct catccccacc
     2761 gtccggggca tccggaagcg caaggacatc cagatcaacc tcgaccacct ctcccacctg
     2821 gtgaaggccg cctcgtggct gtcgagcctc gacctgccgg tccgcctcat cgccttcccg
     2881 gagggcgcgc tgcaggcctt caacgacgag gtgctcgatc tcgaccacgc gaccttcgcg
     2941 cgggagtgcg cgatcgacat cccgggcgag gagaccgaag cgctcggcaa gatcgccaag
     3001 gagtacaacg ccttcgtgat ggcccaggcc aaggcccggc atcccgactg gaaggaccgc
     3061 tacttcaacg tgggcttcat catcaacccg cgcgggcggg tgatcctgca gcattacaag
     3121 gtctcgccgc tcttcccggt ggagcactcg atgtgtcccc acgacgtcta cgactggtgg
     3181 gtggagaaat atggacggac gctccagtcg ttctggccgg tggtcgacac cgagatcggc
     3241 cggctcggca tcatgatggc caacgagggc tcctaccccg agaacgcgcg ggccctcgcc
     3301 ctcaacgggg ccgaggtggt ctaccgcgcc tcctacccgc acccggccac cggcaacgag
     3361 atcttcgaga tccagagccg cgcccgcgcc ctcgacaaca acatgtacct ggtcgccccg
     3421 aacatgggga cctaccacct cttccccgag gagtccaccc ccatcgacac cttcgggggc
     3481 cgctccttcg tcatcgacta caagggccgc atcgtggggc gccaggacta cggggcgggg
     3541 tccacctacg tgggcggggt catcgacgtc gaggccctcc gggaccaccg ggcccgggcc
     3601 cagtgggaca actggatgaa ggacctccgg accgagctct acggtatgct ctacgagaag
     3661 ccgatctatc ccaagaacct ctatctcaag cgcgcaccga tgaagcacaa ggagtatcgc
     3721 gagaagatca tccggcgcca gatccgcctc atgcacgacc ggggcatctg ggccaagccc
     3781 gcacggtagc gatcaccgga gatcagcgac ccatgtccaa cacgagtccg accgccctgc
     3841 tgctcaacgt gggccacgcc atggaccacc tcttcctcct catcttcgcc accgcggtgt
     3901 ccgcgatcgc ggcggagtgg gggatggtct ggcaggacct gatgccctac acggtgggcg
     3961 ccttcgtgct cttcgggctg ggctcgctgc ccgccggccg cctcggcgat ctgtggggcc
     4021 gccgcgccat gatggtgatc ttcttcctgg ggctcggcgc gactggcctc ctggtggccg
     4081 cggcccagcg gccgtggcag ctcgcgatgg ccctcaccgt gatgggcgcc ttcgcggcga
     4141 tctatcaccc ggtcggcatc ccgatgatcg tgcagaactc gacccggccc ggcttcacca
     4201 tcggcctcaa cggcctcgcc ggcaacctgg gcatcgcggt ggccgcggtg ctgaccggct
     4261 tcctggtgca gcgcgtgggc tggcgcatgg ccttcatggt ccccgccctg ctcgcgatcg
     4321 cgagcggggt gctcttcgcc ctggtggtgc cgcgcgagga gatggccccc gcccgcaagc
     4381 cccggcgctc ggtcgacctg cccgcctcga tcatgatgcg gatcttcttc gtcatgaccc
     4441 tcgccgcggt ctcgggcagc atgatcttca acttcaccac caacggcaac aaccagctcc
     4501 tggccgagcg gctgcgcggc ctcgtcgagg acccggccaa gctcggcgtg ctcctggccc
     4561 tggtctacac ggtggcctcc ttcgcccaga tcgtggtggg ccgcctcatc gaccgctacc
     4621 cgctcaagac ggtctacctg ccgatcgtgg ccgcccaggt gccgctcttc ctcctcgccg
     4681 cgaacgcgca gggctgggtc ttctacgcca tcgccgtcgt gttcatggta ttcgtcttcg
     4741 gggccatccc gttcatcgac gcgatgatcg tgcagtacgt ggacgaccgc atgcgctcgc
     4801 gggtggccgg catgcgcctg gccgtctcct tcggggtcag cgcgctcgcg gtgtacctgc
     4861 tgggcccgac cgtgaaggcg gccggcttca ccaacctgct cctgatcatg gccggcatct
     4921 ccgcctccac caccctcttc gtctcgctgc tgcccgggcg catcccgacc gccgcggccg
     4981 cctccgcctc cgccccggcg ccggcgcgct gacgggcgag ccaggccgcg ccatgcccga
     5041 cccggccttc agcccggagg agcatcggct cgcggcgccg cgttggttcg acgacctcac
     5101 ggtgggcgag cgcttcccga ttccctcgcg cacgatgacc gaggcgctct tcgctgcttt
     5161 ccagctcgcc tccgccgaca accacccgat ccactacgat cgcgagtact gccgggccca
     5221 cggccaccgc gatctcctgg cccacggtct cctcgtcacc gcccaggccg cggcgggcgc
     5281 cggcatcttc ccgcatgcga tcagcgactc gctggtggcc ttcctcgacc agtcgtcccg
     5341 cttcctcaag ccggtctacg tgggcgacac gctctacccg ttgctcacca tcacccggct
     5401 cgagcccggg cgcaccaccg gcgtggtcgc catgcgggcc accgtgcaca accaggcgcg
     5461 cgagctggtg atggagggcg agcaccgcta tctcctccgg cggaagccgc cggcctgagc
     5521 cggcgccgcg caccccttcc cgaagcggag caccgaccgt ggccgtccgc gcggacaccc
     5581 gccgctggct gatcgtcgcc gccctcttcg tggtcatcta cggcgtctcg gtgccgctgg
//
LOCUS       DATMCV010000887         3622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_845241, whole genome shotgun sequence.
ACCESSION   DATMCV010000887 DATMCV010000000
VERSION     DATMCV010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845241"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VNU03_20300"
     CDS             complement(<1..528)
                     /locus_tag="VNU03_20300"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP76873.1"
                     /translation="MVETLTDRLTEAWARTDRIFEILAPGSWLAQPIALRHPFIFYLG
                     HLPAFAWNHVGGELLGRPPFNPAFDELFSRGIDPDVDDPTHCHDHPDVPATWPAVPEV
                     LAYRDRVRAALLDAGAAVADRAATHLMAIEGRVFAMIAEHELMHQETLLYMMQQLPLE
                     QKVRPSWVPPLVLGPG"
     gene            609..1583
                     /gene="egtD"
                     /locus_tag="VNU03_20305"
     CDS             609..1583
                     /gene="egtD"
                     /locus_tag="VNU03_20305"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HWP76874.1"
                     /translation="MRYRIDVHTDERAQQQRLEEDVRRGLTSRPKSLPAKYFYDRAGS
                     LLFERITDLPEYYPTRTESTLLAEILPGLIEDFLPDDIVEIGAGSSEKTRRVLDAVTA
                     GGRAVRYVPLDVDRLTLEASAERLLRDYPSLSVHAVVGDFERDLAHVPPPVGRRLAMF
                     LGSTIGNLDEPEQRRLLANLKTLLPDPRDRLLLGVDLVKDVKILEAAYDDSAGVTRDF
                     NLNILRVINRGVDGDFDPDAFRHRAFYNDAASRIEMHLVADSAQTVRLRRLGLTVRFA
                     PGEDIWTESSYKFSRAGVEGLLGGAGLRLARWHVDPANYFALALIQPA"
     gene            1583..2446
                     /locus_tag="VNU03_20310"
     CDS             1583..2446
                     /locus_tag="VNU03_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76875.1"
                     /translation="MAVDGGAYAQPTPLHLYVHDDLSDEIAADAGESSVAAGLARELL
                     ALLADDRERVRVLTLAEQVERVAAQGPHAPFDLTLGIGRAGERVAQALHARTGWFPRV
                     RRIGLTREEDGAGGYRLVSTEGTSLAAQLEGTRACPSLAVVDDTVFSGLTMTGLIEAL
                     DAETRARTRTRAFCLRGVAESVARVAALCPITVGVAAPGRLLAEVSFINASGLVRRVA
                     IRRPERPPLAFFDRPEWIRAWFPTCHADVLALCRRLNALLEPAEPVTSAAPAGSLRDP
                     AARTARGRRAP"
     gene            complement(2373..>3622)
                     /locus_tag="VNU03_20315"
     CDS             complement(2373..>3622)
                     /locus_tag="VNU03_20315"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWP76876.1"
                     /translation="TGTLARVILKDPALQTVEALRARLEAGGLTISASLGFANTYALA
                     IRAEQADRLGLRTVGDLARHPELRAAFSSGFLERDDGWPGLRARYGLGLARVEVMEHA
                     VTYRAIASGQVDLMDVFSTDGQLERLRLRLLEDDRRFFPDYSAVLLARRAMTERFPRT
                     WARLREALEGRLDAARMARLNAMADLDGRSVPEVAAAFLGGGTPEAGGGRGRALLGEL
                     GTLTADHLLLVLISVGAAVVLGIPMGVIAARYRRAGQVELSVIAMLQTVPSLALLMFM
                     IPLFGIGKGPALIALSVYALLPIARNTYAGMMAIEPTLLEIAWVLRLGRLRRLLRVEL
                     PLSAIAIMAGIKTAAVTTVGTATLAAFIGGGGYGTLIVRGLALDDTATILAGAAPAAL
                     MALAFHALFELLDRAVIPRGLRS"
BASE COUNT          512 a         1325 c         1314 g          471 t
ORIGIN      
        1 gcccgggccg agcacgagcg gcggcaccca gctcggccgg accttctgct cgagcggcag
       61 ctgctgcatc atgtagagca gcgtctcctg gtgcatcagc tcgtgctccg cgatcatcgc
      121 gaagacgcgc ccctcgatcg ccatgaggtg ggtggccgcg cgatcggcca ccgcggcgcc
      181 cgcgtcgagc agtgcggcgc gaacccggtc gcggtaggcg agcacctccg gaaccgcggg
      241 ccacgtggcc ggcacgtcgg gatggtcgtg acagtgggtc gggtcgtcca cgtcgggatc
      301 gatcccccgc gagaacagct cgtcgaaggc cgggttgaac gggggccggc cgagcagctc
      361 cccgcccacg tggttccagg cgaaggcggg caagtgtccg agatagaaga tgaacggatg
      421 ccggagcgcg atcggctggg ccagccagga gcccggcgca aggatctcga agatccggtc
      481 cgtgcgggcc cacgcctcgg tcagccggtc tgtcagggtc tcgaccattg ggtcccctcc
      541 cccagaggat ttttcacaac cgcttgcata gtgcctcagt ggcgtctagc ttgcagcagg
      601 agcgtgccat gcgctatcgg atcgacgtgc acacggacga gcgggcccag cagcagcgcc
      661 tcgaggagga cgtccgccga gggttgacct cgcgcccgaa gtccctgccc gcgaagtact
      721 tttacgaccg ggcggggtcg ctgctcttcg agcgcatcac cgacctgccg gagtactatc
      781 cgacccgcac cgagtcgacc ctgctggcgg aaattctgcc cgggctgatc gaggattttc
      841 ttcccgacga catcgtcgag atcggcgccg gctcgtccga gaagacgcgt cgggtgctcg
      901 acgcggtgac cgcgggcggg cgggcggtcc gctacgtgcc cctcgacgtg gaccgcctga
      961 ccctcgaggc gagcgcggag cggctcctgc gcgactaccc cagcctgagc gtgcacgcgg
     1021 tggtgggcga cttcgagcgc gatctcgccc acgtgccgcc gccggtcggc cggcggctgg
     1081 cgatgttcct gggcagcacc atcggaaatc tcgatgagcc ggagcagcgg cgcctgctgg
     1141 cgaatctgaa gacgctcctg cccgacccgc gcgaccggct gctcctgggg gtggacctgg
     1201 tgaaggacgt caagatcctg gaagccgcct acgacgactc ggcgggggtg acgcgcgatt
     1261 tcaatctcaa catcctgcgg gtcatcaacc gcggggtgga cggcgacttc gatcccgacg
     1321 cgttccggca ccgcgccttc tacaacgacg cggcctcgcg catcgagatg cacctggtgg
     1381 cggactccgc ccagacggtg cgtctgcggc gcctgggcct cacggtccgc ttcgcgcccg
     1441 gcgaggacat ctggaccgag agctcgtaca agttcagccg cgccggggtc gaggggctgc
     1501 tcggcggcgc gggcctgcgg ctcgcccgct ggcacgtcga tcccgccaac tacttcgcgc
     1561 tcgccctgat ccagcccgcc tgatggcggt ggacggcggc gcgtacgcgc agccgacacc
     1621 gctccacctc tacgtccacg acgacctgag cgacgagatc gcggccgacg cgggcgagag
     1681 ctcggtcgcc gccggcctcg cgcgcgagct gctcgcgctc ctcgcggacg accgagagcg
     1741 ggtgcgcgtc ctcaccctgg ccgagcaggt ggagcgcgtc gcggcccagg gcccgcacgc
     1801 gccgttcgac ctgaccctcg gcatcggccg cgcgggcgag cgggtggcgc aggcgctcca
     1861 cgcgcgcacc ggctggttcc cgcgcgtccg ccgcatcggg ctcacgcgcg aggaggacgg
     1921 cgcgggcggc tatcgcctgg tcagcaccga ggggacgagc ctcgcggccc agctcgaggg
     1981 gacgcgcgcc tgcccatcgc tggcggtggt ggacgacacc gtcttctccg gcctcaccat
     2041 gaccggcctg atcgaggcgc tcgacgccga gacccgcgcc cgcacccgca cccgcgcgtt
     2101 ctgcctgcgc ggggtggccg agtccgtcgc gcgggtcgcc gcgctctgcc cgatcacggt
     2161 cggcgtcgcc gcacccggcc ggctgctcgc cgaggtgagc ttcattaacg cgagcgggct
     2221 cgtccggcgc gtggcgatcc gccggccgga gcggcccccg ctcgccttct tcgaccgccc
     2281 ggagtggatc cgcgcgtggt tcccgacgtg tcacgccgac gtcctcgcgc tctgccgacg
     2341 cctcaacgcc ctgctcgagc cggccgagcc cgtcacgagc gcagcccccg cgggatcact
     2401 gcgcgatcca gcagctcgaa cagcgcgtgg aaggcgagcg ccatgagcgc ggcgggcgcc
     2461 gcgccggcca ggatggtggc ggtgtcgtcg agggccaggc cccggacgat cagggtgccg
     2521 taaccgcccc cgccgatgaa cgcggccagg gtggcggtgc ccaccgtggt caccgccgcg
     2581 gtcttgatcc cggccatgat cgcgatcgcg gagagcggca gctcgacgcg cagcagccgg
     2641 cgcaggcggc cgaggcgcag cacccaggcg atctcgagca gggtcggctc gatcgccatc
     2701 atcccggcgt aggtgttgcg cgcgatcggc aggagcgcgt agaccgagag cgcgatcagc
     2761 gccgggccct tgccgatgcc gaagagcggg atcatgaaca tcagcagcgc gagcgagggg
     2821 acggtctgga gcatcgcgat caccgacagc tcgacctgcc ccgcgcgccg gtagcgcgcg
     2881 gcgatcacac ccatggggat gcccagcacc accgccgcgc ccaccgagat cagcaccagc
     2941 agcaggtgat cggcggtcag cgtgcccagc tcgccgagca gcgcgcgccc gcgcccaccc
     3001 cccgcctccg gggtgccgcc gccgaggaac gcggcggcca cctcgggcac cgaccggccg
     3061 tccaggtcgg ccatcgcgtt gagccgcgcc atgcgggccg cgtcgaggcg gccctcgagc
     3121 gcctcccgca gccgcgccca cgtgcggggg aagcgctcgg tcatcgcacg gcgcgcgagg
     3181 agcaccgccg agtagtcggg gaagaagcgc cgatcgtcct ccagcaggcg caggcggagc
     3241 cgctcgagct gaccgtcggt ggagaagacg tccatcaggt ccacctggcc gctcgcgatc
     3301 gcgcggtagg tcaccgcgtg ctccatcacc tccacgcggg cgagcccgag cccgtagcgc
     3361 gcgcgcaggc ccggccagcc gtcgtcgcgc tcgaggaagc cggagctgaa ggcggcccgc
     3421 agctccgggt gccgcgccag gtcgccgacg gtgcggagcc cgaggcggtc ggcctgctcg
     3481 gcccggatcg cgagcgcgta ggtgttggcg aacccgagcg aggcgctgat cgtgagccct
     3541 cccgcctcga gccgggcccg gagcgcctcg acggtctgga gcgccggatc cttgaggatg
     3601 acgcgggcga gggtgccggt gt
//
LOCUS       DATMCV010000888         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_182413, whole genome shotgun sequence.
ACCESSION   DATMCV010000888 DATMCV010000000
VERSION     DATMCV010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182413"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VNU03_20320"
     CDS             complement(<1..332)
                     /locus_tag="VNU03_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76877.1"
                     /translation="MRLLSAPFDPAAAPPPRAPLRPFAQTGGVAALVVGLGAASEMLG
                     AVPTGLGRALAMGAAHGGLIGLGWLLATGGAPGWTGPVIRLGVVMTLVSVAARLVPWG
                     ALAFVLMPL"
     gene            complement(307..1062)
                     /locus_tag="VNU03_20325"
     CDS             complement(307..1062)
                     /locus_tag="VNU03_20325"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWP76878.1"
                     /translation="MAGSVGLSQALSYARDLKSLYETSRARERELEQAQERLRHAYAQ
                     SRQYAVDLRKTYRRLQHAIFQSLLGLANALEAKDAYTRGHSERVAALARQVALAAGVS
                     PAEADIIAQAGLLHDLGKIGIPEQVLRKPGPLSAEEWTVMRRHPIVGAQIVAPLEFFA
                     DGAIIVRHHHERHDGSGYPDGLRGELIPLGSRIVAVADVYDALTSDRTYRPRLSRPET
                     VRQLQTEAGRTLDAHLTALCIQVTDDAAAERAV"
     gene            complement(1047..1433)
                     /locus_tag="VNU03_20330"
     CDS             complement(1047..1433)
                     /locus_tag="VNU03_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP76879.1"
                     /translation="MMTLLIADDEPAILDLVRFTLEDPQVRIVEASDGAAALDLAQQL
                     RPDVALLDVKMPRLSGLDVCRRLRELPECAHTRIVLLTAAAQVEDRRRGLAAGADHYL
                     TKPFSPLALLTLVRSLMPEVSIWPAV"
     gene            complement(1570..1983)
                     /locus_tag="VNU03_20335"
     CDS             complement(1570..1983)
                     /locus_tag="VNU03_20335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWP76880.1"
                     /translation="MLLELATPTRQLVSEEVDEVVAPGSEGYFGVLPGHAAFLATLGS
                     GEVVYRRGRDEVHLAVHGGFAEVTPERVIILAETAERPDEIDRARAERARQRAEQRMT
                     GRDPDGSEGEIDYDDAVAAYQRALTRLLVVAIGGG"
     gene            complement(1983..>2804)
                     /gene="atpD"
                     /locus_tag="VNU03_20340"
     CDS             complement(1983..>2804)
                     /gene="atpD"
                     /locus_tag="VNU03_20340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWP76881.1"
                     /translation="LESGEPVISKAALIYGQMNEPPGARARVALSALTAAEYFRDEEG
                     QDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLGTEMGALQERITSTKKGSITS
                     VQAIYVPADDITDPAPATAFAHLDATTVLSRQLTELGIYPAVDPLSSTSRILDPIILG
                     QEHYGTARAVQSILQRYKDLQDIIAILGMEELSDEDKLIVARARKLQRFLSQPFFVAE
                     VFTGQPGRYVKLADTIKGFKEIVDGKHDELPEQAFYMVGGIEEVQEKAAKMREAK"
BASE COUNT          388 a          978 c         1047 g          391 t
ORIGIN      
        1 agtggcatca ggacgaacgc gagagcgccc caggggacga gccgcgcggc gacggacacg
       61 agggtcatga ccaccccgag cctgatgacg ggcccggtcc agccgggcgc gccgcccgtc
      121 gccagcagcc agcccagccc gatcaggccc ccgtgcgccg cccccatcgc cagcgcgcga
      181 ccgagcccgg tcggcacggc gccgagcatc tccgacgcgg cgcccaggcc gacgaccagc
      241 gcggccacgc cgccggtctg cgcgaacggc cggagcggcg cgcgcggcgg cggcgcggcg
      301 gccggatcaa acggcgcgct cagcagccgc atcgtccgtg acctggatgc agagggcggt
      361 gagatgcgcg tcgagggttc gtcccgcttc cgtctggagc tgtcgcacgg tctccggtcg
      421 cgacagacgt ggccggtagg tgcggtccga cgtgagcgca tcgtagacgt cggcgaccgc
      481 cacgatgcgc gagccgagcg ggatcagctc cccgcgcaac ccgtccgggt agccgctgcc
      541 gtcgtgccgc tcgtggtgat gccggacgat gatcgcgccg tcggcgaaga actcgagcgg
      601 cgccacgatc tgcgcgccga cgatcgggtg ccggcgcatc accgtccact cctcggcgct
      661 gagcgggccg ggcttgcgca gcacctgctc cgggatgccg atcttgccga ggtcgtggag
      721 caggcccgcc tgcgcgatga tgtccgcctc cgcgggcgat acgccggccg cgagcgcgac
      781 ctggcgggcg agcgccgcca cgcgctccga gtggccccgg gtgtaggcgt ccttggcctc
      841 gagcgcgttg gcgagcccga gcaggctctg gaagatcgcg tgctggagcc ggcggtacgt
      901 cttgcgcagg tcgaccgcgt actggcgcga ctgcgcgtag gcgtgccgga gccgctcctg
      961 ggcctgctcg agctcccgct cgcgcgctcg cgaggtctcg tagagcgact tgaggtcgcg
     1021 cgcgtaggac agcgcctgcg agaggcctac actgccggcc atatcgacac ctccggcatg
     1081 agggatcgga cgagcgtcag cagggccagc ggcgagaacg gcttggtgag gtagtggtcc
     1141 gcgccggcgg ccagcccccg ccgccgatcc tcgacctggg ccgccgcggt gagcagcacg
     1201 atgcgggtgt gcgcgcactc cggcagctcg cgcaggcgcc ggcacacgtc gaggccgctc
     1261 agccggggca tcttcacgtc gagcagtgcc acgtccgggc gtagctgctg cgcgaggtcg
     1321 agcgcggcgg cgccgtcgga cgcctcgacg atccgcacct gcgggtcctc gagggtgaag
     1381 cggacgagat cgagaatcgc gggctcatcg tcggcgatca ggagggtcat catgccctcg
     1441 ctgattgcga gggacgtgcc accgacggag gtacggcgaa tcagccgctt ggaatcaatc
     1501 ccgcgcggga atgcccggcc ggctgggcag ggcggcgcgc gcggcgcccg accggagagg
     1561 cagccgcttc tagccgccgc cgatcgcgac gaccagcagc cgcgtcaggg cccgctggta
     1621 ggccgccacc gcgtcgtcgt agtcgatctc gccctcggag ccgtccgggt cgcggccggt
     1681 catgcgctgc tcggcgcgct ggcgcgcgcg ctccgccctc gcccggtcga tctcgtccgg
     1741 gcgctccgcg gtctcggcga ggatgatcac ccgctccggc gtcacttccg cgaacccgcc
     1801 gtgcacggcc aggtgcacct cgtcgcgtcc ccggcgatag accacttcgc cgctgcccag
     1861 ggtggccagg aacgcggcgt ggccgggcag gaccccgaag tagccctcgg agccgggcgc
     1921 caccacctcg tcgacctcct ccgagacgag ctggcgcgtg ggagtggcga gctccagcag
     1981 cactacttgg cctcgcgcat cttggccgcc ttctcctgta cctcctcgat gccgcccacc
     2041 atgtagaacg cctgctcggg cagctcgtcg tgcttgccgt ccacgatctc cttgaagccc
     2101 ttgatggtgt ccgcgagctt gacgtagcgg ccgggctggc cggtgaacac ctcggccacg
     2161 aagaagggct gcgagaggaa gcgctgcagc ttgcgggcgc gggccacgat cagcttgtcc
     2221 tcgtcggaga gctcttccat gccgaggatc gcgatgatgt cctggaggtc cttgtagcgc
     2281 tgcaggatgg actggaccgc gcgcgccgtc ccatagtgct cctggcccag gatgatcggg
     2341 tcgaggatgc gcgacgtcga cgagagcgga tccaccgcgg ggtagatgcc gagctcggtg
     2401 agctggcgcg agagcacggt ggtggcgtcc agatgcgcga acgcggtggc gggcgcgggg
     2461 tcggtgatgt cgtcggcggg cacgtagatc gcctgcaccg acgtgatcga gcccttcttg
     2521 gtcgacgtga tccgctcctg cagcgcgccc atctcggtgc cgagggtggg ctggtagccg
     2581 accgcggagg gcatgcggcc gagcagggcg gacacttccg acccggcctg ggtgaagcgg
     2641 aagatgttgt cgacgaagag gagcacgtcc tggccctcct cgtcgcggaa gtactcggcg
     2701 gcggtgagcg ccgagagggc cacgcgggcg cgggcgccgg ggggctcgtt catctggccg
     2761 tagatgaggg cggccttgga gatgacgggc tcgccgctct ccag
//
LOCUS       DATMCV010000889         5315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_453917, whole genome shotgun sequence.
ACCESSION   DATMCV010000889 DATMCV010000000
VERSION     DATMCV010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453917"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4692)
                     /locus_tag="VNU03_20345"
     CDS             complement(<1..4692)
                     /locus_tag="VNU03_20345"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HWP76882.1"
                     /translation="MRVARRFTKEGQSPYAGIQFEQRVSEIKNPDGSTVFRQDGISVP
                     AGWSGVATDILAQKYFRKSGVPQYALDGKPLLDREGRPVLGGERDARQVFHRLAGCWM
                     HWGEKYGYFDTPSDARAFYDELCSMLASQVAAPNSPQWFNTGLHYAYGLSGPAQGHYY
                     VDPDTGILTRASSAYERPQPHACQPYHALVSTPEGPLPIGQIVTKHLVGLEVYDGRTE
                     GTGTTRVVAVQENGEKAVFRIELKNGVAVEATGDHLVWAIVNGVGAWRRVDEVEPGAA
                     LCLSTRTEVRSGSQESLMDEAALVGWLQADGFVGQDGLATDHSLAVEFTTIDKDEMDF
                     VVERVHRVFEGVHYHVRSMETRTPGLDIHRIRLYGERLRPFVEKYGLLRSSADHAVPP
                     MILRAGREAQAAYLRALFQAQGTVELRSYWARLADITLTTTSAGLAHGIQALLLNLGI
                     YSRVGRGAETREGRRTPYVVSIAHAEPRARFRDLIGFVSDDKRELLEIACSDQFAGKR
                     IGALREEMVVRVECVGVQPVYDIQTESGQYLSNNVIVHNCFIQSVADDLVNDGGIMDL
                     WTREARIFKYGSGSGTNFSSLRGENEPLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTR
                     RAAKMVILDLDHPDILNFIRWKVVEEQKVAALVTGSRLTKRRLQSVLGAARTPAGLEA
                     DPRKNPALRSAVREARAAMVPEGYIQRVLQLAGQGVRELDFPEYDTDWDSEAYYTVSG
                     QNSNNSVRVPNSFYDVLARRGQWELKRRTDGKSAGFVPAEQLWDVIAHAAWACADPGV
                     QYDTTINEWHTCPEDGRINASNPCVTGDTLVATAEGWRRIDALVGGTAQVIGADGQPH
                     LVTRIFPTGTKPVFDLRTRLGYRVRITGDHRVLTVGRGDVAVQDLTAGNRVLLRGAGF
                     GRRSLSTGQALGIGVAAAEGYLSHSVIGGRAQDSLVLTTDAGEAPVLAAVASAVNAGR
                     AALQAVGAGGRSESAGVITRTAATARLVLSETSTVDLFREFAVLDEGAANIRFTPAVF
                     ELDRPALASVLRGLFTADAGVANYGEQSRYISLDSTSLELLQQTQLLLLGFGIKARLY
                     EDTRLAEAQPSRPHPTHSLRISRTSRVLFEQEIGFHAESPKAAALAHINGEEPAGGED
                     LTDEVASIEPAGEAPVFDLTEEVTQHFIASGVVVHNCSEYMFLDDTACNLASINLVKF
                     LREDGSFDVDGFRHACRLWTAVLEISVLMAAYPSPAIAQRSWDYRTLGLGYANMGTVL
                     MRKGIPYDSPEATAICGAVTAVMCGEAYATSAEMARDLGPFPGFPRNHDAMLRVMRNH
                     RRAAYRTPAAEYEGLSITPHGIDPAHCPTPLLQAAREAWDRAVTLGEQFGYRNAQVTV
                     LAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINQSLPVALESVGYAPGQIE
                     DIVAYCVGRKTLRGAPFINHDTLRAKGFDDEGLQRIEAGIETAFDISFAFNAWSLGEG
                     YIAAHLGLNEAQLAEWNGNLLRALGFTAAEIEAANDYCCGTMTVEGAPHLRAEHLAIF
                     DCASRCGKRGVR"
     gene            complement(5081..>5315)
                     /locus_tag="VNU03_20350"
     CDS             complement(5081..>5315)
                     /locus_tag="VNU03_20350"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HWP76883.1"
                     /translation="VRDGIGTAVDGAMDPLSYVLPFIWTRLGMKIGNTINDRSLNLDL
                     YQGFEETTVDLYSAVRNGYLQRRENMIKGPAPY"
BASE COUNT          780 a         1835 c         1876 g          824 t
ORIGIN      
        1 ccgcaccccc cgcttgccgc agcggctggc gcagtcgaag atcgcgagat gctccgcccg
       61 gaggtgcggg gctccctcca ccgtcatggt gccgcagcag tagtcgttgg ccgcctcgat
      121 ctccgccgcg gtgaacccca gcgcccgcag gaggttgccg ttccactccg cgagctgcgc
      181 ctcgttcagg cccaggtgcg ccgcgatgta gccctcgccg agcgaccagg cgttgaaggc
      241 gaaggagatg tcgaaggcgg tctcgatccc ggcctcgatc cgctgcagcc cctcgtcgtc
      301 gaagcccttg gcgcggagcg tgtcgtggtt gatgaacggc gcgccgcgca gggtcttgcg
      361 gcccacgcaa taggccacga tgtcctcgat ctggcccggg gcgtagccga ccgactcgag
      421 cgccaccggc aggctctggt tgatgatctt gaagtacccg ccgccggcca gcttcttgaa
      481 cttcaccagc gcgaagtccg gctcgatgcc ggtggtgtcg cagtccatga ccaggccgat
      541 cgtgccggtc ggggccagca ccgtcacctg ggcgttgcgg tagccgaact gctccccgag
      601 ggtcaccgcc cgatcccagg cctcacgcgc cgcctgcagc agaggagtcg ggcagtgggc
      661 cgggtcgatg ccgtgcgggg tgatgctgag cccctcgtac tccgcggcgg gcgtccggta
      721 ggccgcgcgg cggtgattgc gcatgacccg gagcatcgcg tcatggttgc gcgggaagcc
      781 cgggaagggg cccaggtcgc gggccatctc cgcggaggtc gcgtaggcct cgccgcacat
      841 caccgcggtg accgccccgc agatcgcggt cgcctccggc gaatcgtacg ggatgccctt
      901 gcgcatgagc acggtgccca tgttggcgta gccgagcccg agggtgcggt agtcccagct
      961 ccgctgggcg atggccggac tcgggtaggc cgccatcagc acgctgatct cgagcaccgc
     1021 cgtccacagc cggcacgcgt gccggaagcc gtcgacgtcg aaggagccgt cctcgcgcag
     1081 gaacttcacg aggttgatcg aggccaggtt gcacgcggtg tcgtcgagga acatgtactc
     1141 cgagcagttg tgcacgacga cgccgctcgc aatgaagtgc tgggtcacct cctcggtcag
     1201 gtcgaagacc ggggcctcgc cggccggctc gatggatgcg acctcgtcgg tcaggtcctc
     1261 gccgccggcc ggctcctcgc cgttgatgtg ggcgagggcg gccgccttgg gactctccgc
     1321 gtggaagccg atctcctgct cgaagagcac gcgggaggtc cggctgatcc gcagcgagtg
     1381 cgtcgggtgc gggcgggagg gctgcgcctc ggccagccgc gtgtcctcgt agagccgcgc
     1441 cttgatcccg aagcccagga gcaggagctg ggtctgctgc agcagctcga gcgaggtgga
     1501 gtcgagcgag atgtagcgcg actgctcgcc gtagttggcc acgcccgcgt ccgcggtgaa
     1561 gaggccgcgg agcaccgagg ccagcgcggg ccggtccagc tcgaagaccg ccggggtgaa
     1621 gcggatgttc gcggcgccct cgtcgaggac cgcgaactcc cggaagagat cgacggtgga
     1681 ggtctcgctc aggacgagcc gggcggtcgc cgcggtccgg gtgatgaccc ccgcgctctc
     1741 cgatcgtccg cccgctccca ccgcctgcag ggcggctcgc cccgcgttga ccgcgctcgc
     1801 gacggcggcc agcaccggcg cctcgcccgc gtcggtggtg agcacgagcg agtcctgggc
     1861 gcgcccgccg atcaccgagt gcgagaggta gccctccgcg gcggccaccc cgatgccgag
     1921 cgcctgcccg gtcgagaggc tccggcggcc gaagccggcc ccgcggagca ggacccggtt
     1981 gcccgcggtg agatcctgca ccgccacgtc accgcggccg accgtgagga cccgatggtc
     2041 ccccgtgatc cgcacccggt agcccaggcg cgtccgcagg tcgaacaccg gcttggtgcc
     2101 ggtggggaag atgcgggtca ccaggtgcgg ctgcccgtcg gcgccgatca cctgcgcggt
     2161 cccgcccacc agcgcgtcga tccgccgcca gccctccgcg gtggccacca gcgtgtcgcc
     2221 ggtcacgcac gggttgctcg cgttgatgcg cccgtcctcc gggcaggtgt gccactcgtt
     2281 gatggtcgtg tcgtactgca cgcccgggtc ggcgcaggcc cacgcggcgt gcgcgatcac
     2341 gtcccagagc tgctcggcgg ggacgaaccc ggcgctcttg ccgtcggtgc ggcgcttgag
     2401 ctcccactgg ccgcggcgcg ccagcacgtc gtagaaggag ttgggcaccc gcacgctgtt
     2461 gttggagttc tggccggaga ccgtgtagta ggcctcgctg tcccagtcgg tgtcgtactc
     2521 cgggaagtcg agctcgcgca cgccctggcc ggccagctgc agcacccgct ggatgtagcc
     2581 ctccggcacc atggccgcgc gcgcctcgcg caccgccgag cggagcgccg ggttcttgcg
     2641 cgggtcggcc tcgagaccgg cgggggtgcg ggctgcgccc agcaccgact ggagccggcg
     2701 cttggtgagc cgggagccgg tcaccagggc cgccaccttc tgctcctcca ccaccttcca
     2761 gcggatgaag ttcaggatgt ccgggtggtc gagatcgagg atgaccatct tggccgcgcg
     2821 ccgggtggtg ccgcccgact tgatggcgcc ggcggcgcga tcgccgatct tgaggaacga
     2881 catcaggccc gaggacttgc cgccgcccga caggggctcg ttctcgcccc ggaggctcga
     2941 gaagttggtc ccgctgccgg agccgtactt gaagatgcgg gcctcccgcg tccagaggtc
     3001 catgatgccg ccgtcgttga ccagatcgtc ggccaccgac tggatgaagc agttgtggac
     3061 gatcacgttg ttggagaggt actggccgct ctcggtctgg atgtcgtaga ccggctggac
     3121 ccccacgcac tccacccgta ccaccatctc ctcgcgcagc gcgccgatcc gcttgcccgc
     3181 gaactggtcg gagcaggcga tctcgagcag ctcgcgcttg tcgtccgaga cgaagccgat
     3241 caggtcccgg aagcgggcgc gtggctcggc gtgcgcgatg gagaccacgt agggggtgcg
     3301 gcggccctcg cgcgtctccg ccccgcgccc gacccgcgag tagatcccca ggttgagcag
     3361 gagcgcctgg ataccgtggg ccagaccggc ggaggtggtg gtgagcgtga tgtcggccag
     3421 gcgcgcccag taggagcgca gctcgaccgt gccctgggcc tggaagagcg cgcgcaggta
     3481 cgcggcctgg gcctcgcggc cggcccgcag gatcatgggc ggcaccgcat gatccgcgct
     3541 cgaccggagc aggccgtact tctcgacgaa cgggcgcagc cgctccccgt agaggcggat
     3601 gcggtggatg tcgaggcccg gggtccgggt ctccatcgac cggacgtggt agtggacgcc
     3661 ctcgaagacg cggtgcaccc gctccacgac gaagtccatc tcgtccttgt cgatggtcgt
     3721 gaactcgacg gccaggctgt gatcggtcgc gagcccgtcc tggcccacga agccgtcggc
     3781 ctgcagccac cccaccaggg cggcctcgtc catcagggac tcctgggagc ccgagcggac
     3841 ctcggtccgg gtggagaggc acagggccgc gcccggctcc acctcgtcca cccgccgcca
     3901 ggcgccgacc ccgttgacga tggcccacac gagatggtcc ccggtcgcct cgaccgccac
     3961 cccgttcttg agctcgatcc ggaagaccgc cttctcaccg ttctcctgca ccgccaccac
     4021 ccgggtcgtc ccggtgccct ccgtccggcc gtcgtagacc tcgaggccga ccaggtgctt
     4081 ggtcacgatc tggccgatgg gcagggggcc ctccggggtc gagaccaggg catggtaggg
     4141 ctggcaggcg tgcggctggg ggcgctcgta ggcggagctg gcccgggtca ggatcccggt
     4201 gtccgggtcg acgtaatagt ggccctgggc cggcccgctg aggccgtagg cgtagtggag
     4261 accggtgttg aaccactggg gtgagttggg cgccgcgacc tgggaggcga gcatggagca
     4321 gagctcgtca tagaaggcgc gcgcgtccga cggagtgtcg aaatagccat acttctcgcc
     4381 ccaatgcatc cagcagcccg ccagccggtg gaagacctgg cgggcgtccc gctcgcctcc
     4441 caggacgggc cggccctccc ggtcgagcag gggcttgccg tccagggcgt actggggaac
     4501 ccccgacttt cggaagtact tctgggccag gatgtcggtc gccacgcccg accacccggc
     4561 cggcaccgag atcccatcct ggcggaagac cgtcgagccg tccgggttct tgatctccga
     4621 cactcgttgt tcgaactgga tccccgcgta gggactctgg ccttccttgg tgaaccgacg
     4681 ggcgactctc atgatctcct cctagaacac gcgggccaaa ccacgaccgc tgctcgatcc
     4741 attggtgcga acaacttcag acgggaaaaa cgggcttgcg cgacgcgagc gcgtctggac
     4801 tcggaaccga ccgatgtgaa tgcctgttca tatatctagt gtgtgttcaa aagcggaccc
     4861 caacatattg cggcccgcga gaccctgtca actaaaaaag acccgcacaa cttgcacgca
     4921 tcgcgttagc cgttggggat tgagcgaaac cgacgggatc agccgggcgg gttactacga
     4981 gaggcgcgtt gagggaccgc tttcgagcgc cggcttcgcc ggcgcaatcc taaatccgag
     5041 gggggaggcc tcggaggggg ccgtcgaggc cccctccgat ttagtagggt gccggcccct
     5101 tgatcatgtt ctcgcggcgc tggaggtagc cgttccgcac cgcgctgtag aggtcgacgg
     5161 tggtctcctc gaagccctgg tagaggtcca ggttgaggga ccgatcgttg atcgtgttgc
     5221 cgatcttcat cccgagcctg gtccagatga agggcagcac gtaactgagt ggatccatgg
     5281 caccatccac cgcggtcccg atcccgtcac gcacg
//
LOCUS       DATMCV010000890        12003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_583205, whole genome shotgun sequence.
ACCESSION   DATMCV010000890 DATMCV010000000
VERSION     DATMCV010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583205"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2567)
                     /gene="ppdK"
                     /locus_tag="VNU03_20355"
     CDS             complement(<1..2567)
                     /gene="ppdK"
                     /locus_tag="VNU03_20355"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HWP76884.1"
                     /translation="MAGRRETARRTAGAASARAPKYVYAFANGKADGSSALRPLLGGK
                     GCELAEMTKLGVPVPPGFTITTEAWAVYNASGKKHPAGLWKQVLAHLARLEAAAGSRL
                     GDPARPLLVSVRSGARASMPGMMDTVLNLGLNDKSVEGLAQRTRNDRFAWDCYRRFIT
                     LFADVVLSLDRHALDALLDAAKARAKARTDADLPADALRALVSELKAVVQEKTGRPFP
                     QDPLEQLRLAINAVFDSWWAKKAVDYRRINRLSDDWGTAVTVMAMVFGNLGPTSGTGV
                     CFSRDPSSGERRFFGEFLVNAQGEDVVAGIRTPEPIDALKQKMPAVYAKLAAIKDRLE
                     AHYRDMQDIEFTVQDGRLFILQTRSGKRTGAAAVRIAVEMATREKRIDRDTALLRVEP
                     VSLHQLLVKTVDPQAKYTSIAKGLPATPAAAVGKVVFDPEKAVEMALREEKVILVRAE
                     TSPEDVAGMHAAQGVLTSRGGLTSHAAVVARGWGKCCVVGAGDVVVDEENHLFRAGRA
                     VVREGQVVTLNGATGEVIVGAQPLVDPTFSGEFKTLLGWARDRATTRVRANADTPADA
                     TKAREFGAEGIGLVRTEHMFFGDERIPIVREMIMALDVQARKKAVDRLLPFQREDFIG
                     IFRAMAPHPVTIRLLDPPLHEFLPKYKEVLEEYTRLDARGFNPERHAELGALKARLEA
                     LQEANPMLGHRGCRLGITFPEIYDMQVRAIMEAACQVAGEGLSVEPEIMIPLTGTVAE
                     MKLTREMTDRVARQVVAETGRDVRYSVGTMIEVPRAALVADRIAEHAEFFSFGTNDLT
                     QLTYGYSRDDIGKFLPFYLEKKLLPHDPFAVLDQRGVGELVRIGIERGRQTVPTLKVG
                     "
     gene            complement(2752..3498)
                     /locus_tag="VNU03_20360"
     CDS             complement(2752..3498)
                     /locus_tag="VNU03_20360"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWP76885.1"
                     /translation="MDLEPIKSTRIYEEIVRQVKQLIAEGRLKSGDRLPPERDLAEKF
                     MVSRTSVREALRALQSRGLIDIRAGEGAFVRDVSVETLIEPLALVILPHREAVGELFE
                     ARRLLEPAIAALAARRATREEISEMERIVEDQAKEVAAGRTGMLQDTALHAAIANSAH
                     NRAITRIVNALMDLLTQSREESLHMPGRPARSHQDHRRILAAVQKRDEVTAHRAMLDH
                     LIAVETLVTGAHGEEHPASGPAGGRRKPRS"
     gene            complement(3554..4255)
                     /locus_tag="VNU03_20365"
     CDS             complement(3554..4255)
                     /locus_tag="VNU03_20365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWP76886.1"
                     /translation="MSVLLDPEFWARLASIIVIDLSLAGDNALVIALAVRSLPAREQW
                     LGRVWGTAAAVVLRLLFIAVVSALLTIPLLRAVGGLLLLWIAIRLVRPGGSAEGHVRH
                     GTSLRQAVWIIVVADVTMSLDNVLAVAAAAHGDMLLVSLGIALSLPIVVWGSGFLARL
                     MTRHAWIIWVGGGILGYVAGEMITDDPLVRSWLGAHADTLDDFVSVLLALGLALLGWW
                     LARAAASRPPARGET"
     gene            complement(4252..4926)
                     /locus_tag="VNU03_20370"
     CDS             complement(4252..4926)
                     /locus_tag="VNU03_20370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HWP76887.1"
                     /translation="MILDTPEFWGRLLEIGILNMLLSGDNAVVIALAVRALPRRQRLL
                     GQLGGTLGAVVLRLLFVGVISMLLRVPLLRLVGGLLLIWIAYRLVRPVAEAEAGRRHA
                     ASFWESVWIIVVADITMSLDNVLAVAAAAHGDMLLVTLGIAMSVPIVVWGSGVLAGLM
                     NRYPWIIWAGGGLLGYVAGDMLLEDPLLVDWLGAVGPALEYPVPGALAVVLTGLGWWM
                     ARPKRA"
     gene            complement(4954..6480)
                     /locus_tag="VNU03_20375"
     CDS             complement(4954..6480)
                     /locus_tag="VNU03_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HWP76888.1"
                     /translation="MGWENLLLGFQVALQPNNLALAVLGITLGTIIGVLPGLGGANGV
                     AILLPLTFSMNPTSAIILLTSLYWGALFGGAITSILFNIPGEPWSVATTFDGYPMARQ
                     GRGGEALTAAFTSSFVGALFSVVLITLFAPLLAEIALKFGPPEFFAIQLLTFSSFIGL
                     GGGNPIKSLVSILIGFILASAGLDIVTGQLRLTFGITELMKGFDFIVAVIGLFGIGEI
                     LLSVEEGLSFRGISAHINPKVVWQTWKTLPRYYRTFVRSAFIGFWMGFKPGGATPASF
                     MSYAFAKRFSKNPEGFGKGELEGVVAPETAAHAAGGASLLPMITLGIPGSPTAAVMLG
                     GLIIWGLQPGPMLFKEKPEFVWGLIASMYTGNVIGVAMVLAFVPFFAAILRIPFPILT
                     PIIVAICAVGSYAVHDSMVDIWYMLLFGVIGYVFKKLDYPLAPLVLALVLGDLAENAL
                     RQSLIMSQGSLLILFQRPISGLISAVALLFFALPVINPYIRRARAGKPPVEVATRHDD
                     "
     gene            complement(6483..6944)
                     /locus_tag="VNU03_20380"
     CDS             complement(6483..6944)
                     /locus_tag="VNU03_20380"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HWP76889.1"
                     /translation="MRTADLTTALILMAGGLLVIWDSLRLGVGWGTDGPQSGFFPFWL
                     AVFLLASCAIIGAQAWRRTSGTPFVTREQLKPVLITLLPSAGFVVLIQFLGLYVATAI
                     FIGFYMRWIGRYGWVPVILLAVLFPVLTFVVFEKWFLVPMPKGPLEAWLGY"
     gene            complement(6969..7964)
                     /locus_tag="VNU03_20385"
     CDS             complement(6969..7964)
                     /locus_tag="VNU03_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWP76890.1"
                     /translation="MTRQWRRLTVIVAVLVAVGTWGLPSAHAAWEPTKPIEFVIPAGT
                     SGGADQMARLIAGIVEKHKFSPRPIIVVNKSGGAGAEGFLHVKGKKADAHTIIITLSN
                     LFTTPLHTGVPFSWKDLTPVARLALDEFILWVNAETPYKTAKEYLAAVKARSGGDRMK
                     MGGTGSAQEDQILTIQLEQATGTKFTYVPFKGGGEVCVNLVGKHVDSTVNNPIECASH
                     WKAARVRPLAVFDTARIAVPEWKDIPTVKESLGADISYLMLRGIFGPPDMPREALEWY
                     QAMLKKVYDAPEFKEYLTQGALKAAWETGPEFVKWLGEADQLHRELMEKGGLLKK"
     gene            complement(8039..9052)
                     /locus_tag="VNU03_20390"
     CDS             complement(8039..9052)
                     /locus_tag="VNU03_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWP76891.1"
                     /translation="MADTAFTNATQILEPFKGVKKVTIEEYFTSGHRTCQGCESALVM
                     KLMVKAAGPRTIVLGSTGCMYVANTTYYTTPWVVPWMHTQLGSSGSAALGTAAGLKAL
                     MKKGKMKSEPINVIAFCGDGGGADMGLGAISATLTHKEYNSLILLYDNESYANTDIQL
                     SGSTPYGANTTFSPPGSVKRIIHTRWKKNTAGMLAAGHPECRYVATVDASYAVEMMNK
                     VRKALTIGGPTFIHSLDPCPKGWDYDPMLSHELGELAVETGIFPLYEVEDGVVRHYGK
                     TKAIVEGRPRKPVREYLLKQGRFAHFTEEDLEYFQSKVDEMWDKWEIPGVIPFWKERP
                     GAK"
     gene            complement(9068..10297)
                     /locus_tag="VNU03_20395"
     CDS             complement(9068..10297)
                     /locus_tag="VNU03_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HWP76892.1"
                     /translation="MAVETKPAGAVADHTEMLISGSEACAEALTLADLDVVTAYPIRP
                     YDTVMQAIAKKIANGHLEAEYIVAEGEHSQFEIVKHASTVGARVFCGSSGVGWMYAME
                     CLTVTPPLRVPMVCMVGNRALDDPGAFGVEHNDALVVRDLGWLLIWVDTAQEMLDTTL
                     LAYRIAEDRRVFLPIAISADGAFLTHSQTICQVPSRALVDKFLPRYDRGDLLLHPDNP
                     ITVAPQANEDWVIEIRRQNDEAMKRATTVIEEAYADFRKVMGRAPENPWFEEYMTDDA
                     EIILVGMGTISLPMKVGIRNMRAQGKKVGLIRPRWFRPFPTERLVKALSKASAIGVID
                     RDYSFGSPFNSGVVANEIRAAMYNADKRPPLLSFICGLGGREVTLEDVAKAADMCYAA
                     AKAGKSDPKTHWLGVRE"
     gene            complement(10313..11518)
                     /locus_tag="VNU03_20400"
     CDS             complement(10313..11518)
                     /locus_tag="VNU03_20400"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HWP76893.1"
                     /translation="MAHFTCEVVYRGIFQKNLAARICRGIVLSARKAGRWGIAFGRYG
                     DSPQRNGIPAKDFAIVADSQEELEQNMARYEPKEVDVTICVDDTLSKGVESWAWYGLQ
                     PINRLTVSGGTVLMTSLQSQAALLKDIHKKDAPYNLALIRAKASFSGLWVYKEDHTEA
                     RILGALARIQPEFLSLDAVCQAITEAEWGSDLKVASARKAHERLETQPIKMTEGNAEI
                     PYSFEKPKWWEMREGVTIPAIPIGKPMEGGKGYVPERNPYFKKYTTRTMRPVIDFDKC
                     VKCTLCWIQCPDSCFDVTPEGLYDANMESCCGCGVCEAVCPAKDCVTMVREDAFEDNA
                     SQWEMWRTDKAKYTTWMTDKIHSKEHITRSHGFRFRGQYEEEIKKDLEQGGIEIIAGI
                     PGENAAGKK"
     gene            complement(11637..>12003)
                     /locus_tag="VNU03_20405"
     CDS             complement(11637..>12003)
                     /locus_tag="VNU03_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003231391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HWP76894.1"
                     /translation="MLDALAARRPLQAGWVAAVNQALGWGTGASRLVRQGRGWRLAFA
                     STLGEPLRALAPIARSMADLAAQGRAVAVRKCANPRCVLYFRDTSRTRRRRWCSMAVC
                     GNRMKVAAHTRRHGRRRSA"
BASE COUNT         1857 a         4155 c         4124 g         1867 t
ORIGIN      
        1 ccgaccttca gcgtgggcac ggtttgccgg ccccgctcga tgccgatccg taccagctcc
       61 cccacgcctc gctgatccag caccgcgaac gggtcgtggg gcagcagctt cttctccagg
      121 tagaacggca ggaacttgcc gatgtcgtcg cggctgtagc cgtaggtcag ctgggtcagg
      181 tcgttggtgc cgaacgagaa gaactcggcg tgctccgcga tccggtccgc gaccagcgcg
      241 gcgcgcggca cctcgatcat ggtgccgacc gagtagcgca cgtcccggcc cgtctccgcc
      301 accacctggc gggcgacccg gtccgtcatc tcgcgggtca gcttcatctc cgccaccgtt
      361 ccggtgagcg ggatcatgat ctcgggctcg accgacagcc cctccccggc cacctggcag
      421 gccgcctcca tgatggcccg cacctgcatg tcgtagatct cggggaacgt gatgccgaga
      481 cggcagcccc ggtgcccgag catcgggttc gcctcctgga gggcctcgag ccgcgccttc
      541 agcgcgccca gctccgcgtg ccgctccggg ttgaagccgc gcgcgtcgag ccgcgtgtac
      601 tcctcgagca cctccttgta cttggggagg aactcgtgga gcggcggatc gagcagccgg
      661 atggtgaccg ggtgcggggc catggcgcgg aagatgccga tgaagtcctc ccgctggaac
      721 ggcagcagcc gatcgaccgc cttcttccgg gcctggacgt cgagggccat gatcatctcg
      781 cgcacgatcg ggatgcgctc gtcaccgaag aacatgtgct cggtgcgcac cagcccgatg
      841 ccctccgccc cgaactcccg ggccttggtc gcgtccgcgg gagtgtcggc gttggcgcgc
      901 acccgggtcg tggcgcgatc ccgcgcccat cccaggagcg tcttgaactc gcccgagaac
      961 gtcggatcca cgagcggctg cgcgccgacg atcacctcgc cggtggcgcc gttcagcgtg
     1021 acgacctggc cctcgcgcac caccgcgcgc ccggcccgga agagatggtt ctcctcgtcg
     1081 acgaccacgt cgcccgcgcc caccacgcag cacttgcccc agccgcgggc gaccaccgcc
     1141 gcgtgcgacg tgaggccgcc ccgcgaggtc agcacgccct gcgccgcgtg catgccggcc
     1201 acgtcctcgg gcgacgtctc ggcccgcacc aggatgacct tctcctcccg cagggccatc
     1261 tcgaccgcct tctcgggatc gaacaccacc ttgccgaccg cggccgcggg ggtggcgggc
     1321 aggcccttgg cgatcgaggt gtacttggcc tgcgggtcga cggtcttgac cagcagctgg
     1381 tggagcgaga ccggctccac ccgcaggagc gcggtgtcgc ggtcgatccg cttctcccgg
     1441 gtggccatct ccaccgcgat gcgcaccgcg gccgcgccgg tccgcttgcc ggagcgcgtc
     1501 tgcaagatga agagccggcc gtcctgcacc gtgaactcga tgtcctgcat gtcgcggtag
     1561 tgggcttcca ggcgatcctt gatcgccgcg agcttggcgt agaccgcggg catcttctgc
     1621 ttgagggcgt cgatcggctc cggggtgcgg atccccgcga ccacgtcctc gccctgggcg
     1681 ttgaccagga actcgccgaa gaagcgccgc tcgccgctcg aggggtcgcg ggagaagcag
     1741 accccggtgc ccgaggtggg gccgaggttg ccgaacacca tcgccatcac ggtcaccgcg
     1801 gtgccccagt cgtcggagag gcggttgatg cgccggtagt cgaccgcctt cttggcccac
     1861 caggagtcga acaccgcgtt gatggccagg cggagctgct cgaggggatc ctgcgggaac
     1921 ggccgcccgg tcttctcctg caccaccgcc ttcagctcgg agacgagggc gcggagcgcg
     1981 tcggcgggca ggtcggcgtc ggtgcgcgcc ttcgcccgcg ccttggccgc gtccagcagc
     2041 gcgtcgaggg cgtgtcggtc gagggacagc accacgtccg cgaacagggt gatgaagcgg
     2101 cggtagcagt cccacgcgaa gcggtcgttc cgggtccgct gcgccagccc ctccaccgac
     2161 ttgtcgttca gaccgaggtt gaggacggtg tccatcatgc ccggcatgga cgcccgcgcg
     2221 ccggagcgca ccgagacgag gagcgggcgc gccggatcgc cgaggcggct ccccgcggcg
     2281 gcctcgagcc gggccaggtg ggcgaggacc tgcttccaca gcccggcggg atgcttcttg
     2341 ccggacgcgt tgtagacggc ccacgcctcg gtggtgatgg tgaagccggg cggcaccggt
     2401 actcccagct tcgtcatctc ggccagctcg caccccttgc cccccagcag cggccgcagg
     2461 gcgctcgatc cgtccgcctt gccgttcgcg aacgcgtaga cgtacttggg agcgcgagcg
     2521 gaggcggctc cggcagtccg gcgggcggtc tctcgacgtc cggccatcaa cgaccccctc
     2581 agcactctcg ggcggtggca gagaaaaaga cacaggcccg gacagcggcc tgcgttagtc
     2641 cgttatgcta ggccgccggg gcggcgatgt caacgatttg atatagctgc atcaggtact
     2701 tacggagagc ttcggcaccg gctttcacac cggtccccgg gtaaccgcga atcaggatcg
     2761 gggttttcgc cggcctccgg cgggcccgga ggccgggtgc tcctctccgt gagcccccgt
     2821 caccagcgtt tccaccgcga tcaggtggtc cagcatcgcg cggtgggccg tcacctcgtc
     2881 gcgcttctgg acggcggcga ggatgcgccg atggtcctgg tgcgagcgcg ccggtctccc
     2941 gggcatgtgc agggactcct cgcggctctg agtgagcagg tccatgaggg cgttgacgat
     3001 gcgggtgatg gcgcggttgt gggcactgtt ggcgatggcg gcgtggagcg cggtgtcctg
     3061 cagcatcccg gtgcgcccgg cggccacctc cttcgcctgg tcctcgacga tgcgctccat
     3121 ctccgagatc tcttcgcggg tcgcgcggcg cgcggcgagg gccgcgatgg ccggctcgag
     3181 cagccggcgc gcctcgaaca gctcgcccac cgcttcccgg tgcggcagga tcaccagggc
     3241 cagcggctcg atcagggtct ccaccgagac gtcccgtacg aacgcccctt ccccggcccg
     3301 gatatcgatc agcccgcggc tctgcagcgc ccggagcgcc tcgcggaccg aggtccggct
     3361 caccatgaac ttctcggcca ggtcgcgctc gggcggcagc cggtcgcccg acttgagccg
     3421 cccctccgcg atcagctgct tgacctggcg gacgatctcc tcgtagatcc gcgtcgactt
     3481 gatcggctca agatccatcc cctccggggg ccgggccccg tcagtccgcc gccgggcgcg
     3541 ctcgctcatc gggtcacgtc tccccccggg cgggcgggcg ggacgcggcg gcccgggcca
     3601 gccaccagcc caggagcgcg aggcccaggg ccagcagcac cgacacgaag tcgtcgaggg
     3661 tgtcggcgtg cgctccgagc cacgagcgca ccagggggtc gtcggtgatc atctctccgg
     3721 ccacgtagcc gaggatcccg cccccgaccc agatgatcca ggcgtgcctc gtcatgagcc
     3781 gcgcgaggaa gccgcttccc cacaccacga tcggcaggga cagcgcgatg ccgagcgaga
     3841 ccagcagcat gtccccgtgc gcggccgcgg ccaccgcgag gacgttgtcg aggctcatgg
     3901 tgacgtcggc caccacgatg atccacaccg cctggcggag cgaggtcccg tgccggacgt
     3961 ggccctccgc gcttccgccg ggccggacca gccggatcgc gatccacagc aggagcaggc
     4021 cgcccaccgc ccgcaggagc ggaatggtga gcagcgcgct caccaccgcg atgaacagca
     4081 ggcgcaggac gaccgcggcc gcggtgcccc acacccgccc gagccactgc tcgcgcgcgg
     4141 gcagcgagcg caccgcgagc gcgatgacca gggcgttgtc gccggccagc gagagatcga
     4201 tgacgatgat gctcgccagc cgggcccaga actcgggatc gagaagcacg ctcatgctcg
     4261 tttgggacgc gccatccacc agcccaggcc ggtcaggacc acggcgagcg cgccgggaac
     4321 aggatactcc agggcgggcc cgaccgctcc gagccagtcc accagcagcg ggtcctccag
     4381 cagcatgtca cccgccacgt agccgagcag gccgccgccc gcccagatga tccacggata
     4441 gcgattcatc agcccggcca ggactccgct gccccacacc acgatgggca ccgacatcgc
     4501 gatgccgagg gtgaccagca gcatgtcgcc gtgcgccgcg gccgccaccg cgaggacgtt
     4561 gtcgaggctc atggtgatgt cggccaccac gatgatccac accgactccc agaacgacgc
     4621 ggcgtggcgg cggcccgcct ccgcctccgc caccgggcgg accagccggt acgcgatcca
     4681 gatcagcagc agcccgccca ccaggcggag cagcggcacg cgcaggagca tcgagatgac
     4741 gcccacgaag agcaatcgca ggacgaccgc gcccagcgtc ccgccgagct gccccaggag
     4801 acgctgccgc cgcggcaggg cccgcaccgc gagcgcgatg accaccgcgt tgtcccccga
     4861 gagcagcatg ttgaggatcc cgatctcgag gaggcggccc cagaactcgg gggtgtcgag
     4921 gatcaccgac gcgcccggag cggcccggcg cgctcagtca tcgtgccggg tcgccacctc
     4981 gaccgggggc ttcccggccc gcgcgcgccg gatgtaggga ttgatcaccg gcagcgcgaa
     5041 gaagaggagg gcgaccgcgc tgatgagccc ggagatgggg cgctggaaca ggatcagaag
     5101 cgagccctgc gacatgatca ggctctggcg gagggcgttc tcggccagat ctccgagcac
     5161 gagggccagg acgaggggag ccagcgggta gtcgagcttc ttgaacacgt aaccgatcac
     5221 gccgaagagg agcatatacc agatgtccac catgctgtcg tggaccgcgt aggagccgac
     5281 cgcgcagatc gccacgatga tcggggtgag gatcggaaac gggatccgga ggatcgccgc
     5341 gaagaagggg acgaaggcca gcaccatcgc gaccccgatc acgttgccgg tgtacatgct
     5401 ggcgatgagc ccccagacga actccggctt ctccttgaag agcatcggcc ccggctggag
     5461 accccagatg atgagccccc ccagcatcac cgccgcggtg gggctgcccg ggatcccgag
     5521 cgtgatcatc ggcagcagcg aggccccgcc cgcggcgtgg gccgcggtct ccggcgccac
     5581 caccccctcg agctcgccct tgccgaagcc ctccgggttc ttcgagaagc gcttggcgaa
     5641 ggcgtagctc atgaacgagg cgggggtggc cccgcccggc ttgaatccca tccagaagcc
     5701 gatgaaggcg ctccggacga aggtgcggta gtagcggggc agcgtcttcc aggtctgcca
     5761 gacgaccttc gggttgatgt gggcgctgat cccgcggaag gagaggccct cctccaccga
     5821 gaggaggatc tcgccgatgc cgaagagccc gatgaccgcc acgatgaaat cgaagccctt
     5881 catcagctcg gtgatcccga aggtcagccg gagctgtccg gtcacgatgt cgagcccggc
     5941 ggaggcgagg atgaagccga tcaggatcga gacgagcgac ttgatcgggt tgccgccccc
     6001 caggccgatg aacgaggaga aggtgaggag ctggatcgcg aagaactcgg gagggccgaa
     6061 cttgagcgcg atctcggcca gcagcggggc gaagagcgtg atcagcacca ccgagaagag
     6121 ggcccccacg aacgaggagg tgaacgcggc ggtgagcgcc tcgccgcccc gcccctgccg
     6181 cgccatcgga tacccgtcga aggtggtggc caccgaccag ggctcgcccg ggatgttgaa
     6241 gaggatcgac gtgatggcgc cgccgaacag cgcgccccag tagaggctcg tgagcaggat
     6301 gatggccgag gtgggattca tcgagaacgt gagcggcagc aggatcgcca ccccgttggc
     6361 gccgcccagg ccgggcagca cgccgatgat ggtgccgagg gtgatgccga gcaccgcgag
     6421 ggcgaggttg ttcggctgga gcgcgacctg gaagccgagg agcaggttct cccagcccat
     6481 gctcagtacc cgagccaggc ctcgaggggg cccttgggca tggggaccag gaaccacttc
     6541 tcgaagacca cgaaggtgag caccgggaag agcaccgcga gcaggatcac cggcacccac
     6601 ccgtagcggc cgatccagcg catgtagaag ccgatgaaga tggcggtggc cacgtagagc
     6661 ccgaggaact ggatcagcac cacgaacccc gcggagggca gcagcgtgat gagcaccggc
     6721 ttgagctgct cccgggtgac gaagggggtg ccgctcgtcc ggcgccacgc ctgcgcgccg
     6781 atgatcgcgc aggacgcgag gaggaagacc gcgagccaga acgggaagaa cccgctctgc
     6841 gggccgtcgg tgccccagcc gacgcccagg cgcagcgagt cccagatgac caggagacct
     6901 cccgccatca ggatcaacgc cgtcgtcagg tcggcggtgc gcacgtccgc gctccggagg
     6961 ctaggctcct acttcttgag gagccccccc ttctccatga gctcgcggtg cagctggtcg
     7021 gcctctccga gccacttgac gaactcgggt ccggtctccc aggctgcctt gagggcgccc
     7081 tgagtgaggt actccttgaa ctcgggcgca tcgtagacct tcttgagcat cgcctggtac
     7141 cactcgagcg cctcccgggg catgtccggc ggcccgaaga tgccccgcag catcaggtag
     7201 ctgatgtcgg cgccgaggga ctccttgacc gtcgggatgt ccttccattc cgggaccgcg
     7261 atccgcgcgg tgtcgaacac cgcgagcggg cgcacccgcg ccgccttcca gtggctcgcg
     7321 cactcgatcg ggttgttgac ggtggagtcg acgtgcttgc ccaccaggtt cacgcacacc
     7381 tcgcccccgc ccttgaacgg cacgtaggtg aacttggtcc cggtggcctg ctccagctgg
     7441 atggtgagga tctggtcctc ctgggccgac ccggtcccgc ccatcttcat ccggtcgccg
     7501 cccgagcgcg ccttgaccgc ggccaggtac tccttcgcgg tcttgtaggg cgtctccgcg
     7561 ttgacccaca ggatgaactc gtcgagggcc aggcgggcga ccggggtcag gtccttccat
     7621 gagaacggca ccccggtatg cagcggggtg gtgaagagat tcgacagggt gatgatgatg
     7681 gtgtgggcgt cggccttctt gcccttgacg tgcaggaacc cctcggcgcc cgcgcccccc
     7741 gacttgttca ccacgatgat cgggcgcgga gagaacttgt gcttctcgac gatgcccgcg
     7801 atgagacgcg ccatctgatc ggccccgccg ctggtgccgg cggggatcac gaactcgatg
     7861 ggcttggtgg gctcccacgc cgcgtgcgcg gaggggagcc cccaggtgcc gaccgcgacc
     7921 aggaccgcaa cgatcaccgt gagacgtcgc cattgacggg tcatatcccc tcctcggggg
     7981 ccgggcgctc gggcgcgcgg gcgacgcaac cgtttgtgcg gcttacgtgc ggtgcggctt
     8041 acttggcgcc gggccgctcc ttccagaacg ggatcacgcc cggaatctcc cacttgtccc
     8101 acatctcgtc caccttggac tggaagtact ccaggtcctc ctcggtgaag tgggcgaagc
     8161 gaccctgctt gaggaggtac tcccgcaccg gcttgcgcgg gcgcccttcc acgatggcct
     8221 tggtcttgcc gtagtgacgc accaccccgt cctccacctc gtagagcggg aagatgccgg
     8281 tctcgaccgc cagctcgccc agctcgtggg agagcatcgg atcgtagtcc cagcccttcg
     8341 ggcacgggtc gagcgagtgg atgaaggtgg gcccgccgat ggtgagcgcc ttgcggacct
     8401 tgttcatcat ctcgaccgcg taggaggcgt ccaccgtcgc cacgtagcgg cactcgggat
     8461 gcccggccgc cagcatgccc gcggtgttct tcttccagcg ggtgtggatg atgcgcttga
     8521 ccgatcccgg cgggctgaac gtggtgttgg cgccgtaggg ggtcgagccg gagagctgga
     8581 tatcggtgtt ggcgtacgac tcgttgtcgt agagcaggat cagcgagttg tactccttgt
     8641 gggtcagggt cgcggagatg gcgcccagcc ccatgtcggc cccgcccccg tcaccgcaga
     8701 aggcgatgac gttgatcggc tccgacttca tcttgccctt cttcatgagc gccttgagcc
     8761 ccgccgcggt gccgagggcg gccgagccgg aggagccgag ctgggtgtgc atccacggca
     8821 ccacccacgg ggtcgtgtag taggtggtgt tggccacgta catgcagccg gtggagccga
     8881 gcacgatggt gcgcgggccc gccgccttga ccatcagctt catcaccagg gccgactcgc
     8941 agccctgaca ggtgcggtgg ccggaggtga agtactcctc gatggtgacc ttcttgacgc
     9001 ccttgaaggg ctcgaggatc tgcgtcgcgt tcgtgaatgc ggtatcggcc atatcggtag
     9061 cctccgatta ctctctgacg cccagccaat gcgtcttggg gtcggatttc ccagccttcg
     9121 cggcggcgta gcacatgtcc gccgccttcg cgacgtcctc cagggtgacc tcgcggccgc
     9181 cgagcccgca gatgaagctg agcagcggcg ggcgcttgtc cgcgttgtac atcgcggccc
     9241 ggatctcgtt ggcgaccact cccgagttga acggcgagcc gaaggagtag tcgcggtcga
     9301 tgacgccgat ggcggaggcc ttcgagagcg ccttcaccag ccgctcggtc gggaacgggc
     9361 ggaaccagcg ggggcggatc aggccgacct tcttgccctg ggcccgcatg ttccggatgc
     9421 ccaccttcat cggcagcgag atcgtcccca tgcccaccag gatgatctcc gcgtcgtcgg
     9481 tcatgtactc ctcgaaccac gggttctcgg gcgcgcggcc catgactttt cggaaatcag
     9541 cataggcttc ttcgatcacg gtggtggccc gcttcatcgc ctcgtcgttc tgccgcctta
     9601 tctcgatgac ccagtcctcg ttggcctggg gcgccaccgt gatcgggttg tccgggtgca
     9661 gtagcaggtc gccccggtcg taacgcggca ggaacttgtc caccagggcg cggctcggca
     9721 cctggcagat cgtctgggag tgcgtgagga aggcgccgtc cgccgagatc gcgatgggca
     9781 gaaagacgcg gcggtcctcg gcgatccggt aggcgagcag cgtggtgtcc agcatctcct
     9841 gggcggtgtc cacccagatc agcagccagc ccaggtcacg gacgaccaga gcgtcattgt
     9901 gctcgacccc gaaggccccc ggatcgtcca gcgcccggtt gcccaccatg cacaccatgg
     9961 gcacccggag cgggggcgtg acggtgaggc actccatcgc gtacatccag ccgacgccgg
    10021 aggagccgca gaacacccgc gccccgacgg tggaggcgtg cttcacgatc tcgaactggc
    10081 tgtgctcgcc ttccgccacg atgtactcgg cctccagatg gccgttggcg atcttcttgg
    10141 cgatcgcctg catgaccgtg tcgtagggcc ggatcgggta cgcggtcacc acgtcgaggt
    10201 cggccagggt cagcgcctcg gcacaggcct cgctgccgga gatcagcatt tccgtgtgat
    10261 cggcgactgc tcctgcgggc ttggtctcca ccgccatggt ctggtctcct ccctacttct
    10321 tccccgcggc gttctcgccg ggaatgcccg cgatgatctc gatgcccccc tgctccagat
    10381 ccttcttgat ctcttcctcg tactggcccc ggaagcggaa gccgtgggac cgggtgatgt
    10441 gctccttgct gtggatcttg tcggtcatcc acgtggtgta cttggccttg tcggtccgcc
    10501 acatctccca ctggctcgcg ttgtcctcga aggcatcctc gcgcaccatg gtgacgcagt
    10561 ccttggccgg gcacaccgcc tcgcagaccc cgcagccgca gcaggattcc atgttggcgt
    10621 cgtagaggcc ctccggggtg acgtcgaagc aggagtcggg gcactggatc cagcagaggg
    10681 tgcacttcac gcacttgtcg aagtcgatga ccggccgcat ggtgcgcgtg gtgtacttct
    10741 tgaagtacgg gttgcgctcg ggcacgtagc ccttgccgcc ttccatcggc ttgccgatgg
    10801 gaatcgcggg gatggtcacc ccctcgcgca tctcccacca cttcggcttc tcgaaggaat
    10861 acgggatctc cgcgttgccc tcggtcatct tgatcggctg ggtctccagc cgctcatgag
    10921 ccttgcgggc cgaggcgacc ttgagatccg agccccactc ggcttccgtg atggcctggc
    10981 agaccgcgtc gagcgacagg aactcgggct ggatcctggc cagcgcgccc aggatccggg
    11041 cctcggtgtg gtcttccttg tagacccaga gccccgagaa ggatgccttg gcccggatga
    11101 gggccaggtt gtagggcgcg tccttcttgt ggatgtcctt gaggagggcg gcctggctct
    11161 ggagcgacgt catgaggacg gtgccgcccg agaccgtcag gcggttgatc ggctgcagcc
    11221 cgtaccaggc ccaggactcg acgcccttgg agagggtgtc gtcgacgcag atggtgacgt
    11281 cgacctcctt gggctcgtag cgggccatgt tctgctccag ctcctcctgc gagtcggcca
    11341 cgatggcgaa gtccttcgcc gggatgccat tgcgctgggg gctgtcaccg tagcgcccga
    11401 aggcgatgcc ccatcgcccc gccttgcggg cggaaaggac gatgccgcga cagatgcgcg
    11461 cggcgaggtt cttctggaag atgccccggt acacgacctc gcaggtgaag tgcgccatgc
    11521 cttcctcctc gaacgagcgg cgttgctttc ttagcctgac gtgtggtcct ggtcggctgg
    11581 tcggaccata ccttgcctcc ggcgagcagg gctgtcaagg gcaaaccggc tccgggctag
    11641 gcggatcggc ggcggccgtg ccgccgggtg tgggccgcca ccttcatccg gttgccgcag
    11701 accgccatcg agcaccagcg gcggcggcgc gtgcgggagg tatcgcggaa atagaggacg
    11761 cagcgcgggt tcgcgcactt ccggacggcc acggcccggc cctgcgcggc caggtccgcc
    11821 atggaccgcg cgatcggggc cagggcccgg agcggctcgc ccagggtcga ggcgaaggcg
    11881 aggcgccagc cccgtccttg ccggacgagc cggctcgcgc cggtgcccca gcccagcgcc
    11941 tggttcaccg ccgcgaccca gccggcctgc agcgggcgcc gggccgccag ggcgtccagc
    12001 atc
//
LOCUS       DATMCV010000891         2430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_115065, whole genome shotgun sequence.
ACCESSION   DATMCV010000891 DATMCV010000000
VERSION     DATMCV010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115065"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1261
                     /locus_tag="VNU03_20410"
     CDS             176..1261
                     /locus_tag="VNU03_20410"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP76895.1"
                     /translation="MGPDSPSGAGGVAGAPGPFEIDERALIQRCIAGDATAFEPLVEK
                     YRQRVWRLAYQVLHDREEAWDVAQEAFVRAFHSLPSFRGQSAFYTWLFRITVNVATDR
                     HRQRGAQARAFGPERVSEEEWARTTPDPGGGPEQQAARKEQRERIRSALDALPPKART
                     IIMLSDVEGLSYREIAEVLNCPIGTVMSRLHNARKRLKVLLGPMLVLMLWLGLTLVLT
                     ASAEAQPVIRFGVRILEASSAPRTPSGPAAPKSEAEPDERLRKIIPRLQTLFRYTDYT
                     TLDRQRVDGPLGTQQRFAVPGERWLEVTPDQMQGTSVRMRVRLLRGERPEMNANILAA
                     PGAPAVLGGPPYGSGVLIIILWANPNP"
     gene            1265..>2430
                     /locus_tag="VNU03_20415"
     CDS             1265..>2430
                     /locus_tag="VNU03_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWP76896.1"
                     /translation="MSEQRYRQERSRIEQGHVKYRDKLKEEGKLFVRDRLKLLLDPGS
                     EFQEDFLFARNQEADTPADGVVTGVGTVGGRTVCVMANDYTVKAGSWGEKTVQKIVRI
                     QERAQRLQVPLLYLVDAAGGRISEQIKIFPGRFHAGRIFYNEVQLSGVVPQVCVLFGP
                     SPAGSAYLPALTDCVIMVDGKASLYVGSPRMVEMAIGEKTTLEELGGARMHCAVSGCG
                     DMLAASDEEAIELCRRYLSYMPGSFRERPAVADAAPAKPGRSIEDIVPYDQRKWFDMY
                     EVIDRIVDEGSFFEIKRLFAAEIIVGFARLAGRAVGLVANQPKVKGGVLMVDSSDKAA
                     RFISLCNAYNIPLVYLADVSGFMVGTKVERAGIIRHGAKMVFATSQATVPKICVV"
BASE COUNT          371 a          819 c          856 g          384 t
ORIGIN      
        1 ccggcttcgg gcccgccagc tacctgctcg gggtggatgc cctgctgggc gcgttgccgg
       61 gggcctccag acgccgcgcc gcctcgcgac tccgacggtg aatgcgggcg cgcttcctga
      121 cgtccaaccc actgtcggat aaggggcgcg gagaccagcg cggggttcgg ccgtgatggg
      181 ccccgactcg ccgtccggcg cgggcggtgt cgcgggggct ccgggcccct tcgagatcga
      241 cgagcgcgcg ctgatccagc gatgcatcgc tggagacgcg accgccttcg agccgctcgt
      301 cgagaagtat cgtcagcggg tgtggcgcct ggcctaccag gtcctgcacg accgcgagga
      361 ggcgtgggac gtggcacagg aggcattcgt acgggcgttt cattcgttgc cgtcgttccg
      421 cgggcagtcg gcgttctaca cctggctctt tcgcatcacc gtcaacgtcg ccaccgatcg
      481 gcatcgccag cggggggccc aggcgcgcgc cttcgggccg gagcgggtga gcgaggagga
      541 gtgggcgcgg acgactccgg atcccggggg cgggcccgag cagcaggcgg cgcggaagga
      601 gcagcgagag agaatccgga gcgctctcga tgccttgccg cccaaggcgc gaacgattat
      661 catgctcagt gacgtcgagg gcctctcgta ccgcgagatc gccgaggtgc tcaactgccc
      721 gatcgggacc gtgatgtcca ggctgcataa tgcacgcaag cggctcaagg tacttctcgg
      781 accgatgctc gtcctgatgc tctggctggg cctgaccctg gtgctcacgg cgtccgccga
      841 ggcccagccg gtcatccgct tcggggtccg catcctcgag gcctccagcg cgcccaggac
      901 cccatccggc cccgccgcgc cgaagtcgga ggccgagccg gacgagcggc tgcggaagat
      961 catcccgcgt ctccagacgc tcttccgcta caccgactac acgacgctcg accgccagcg
     1021 ggtcgacggc ccgctcggga cccagcagcg cttcgcggtg cccggcgagc gctggctcga
     1081 ggtgactccg gaccagatgc agggcacctc ggtccggatg cgggtgcgcc tgctgcgcgg
     1141 cgagcgcccc gagatgaacg ccaatatcct cgccgcgccc ggggctcccg cggtgctcgg
     1201 cggccccccc tacggaagcg gggtgctgat catcatcctc tgggcgaatc cgaatccgta
     1261 gtcgatgagc gagcagcgct atcggcagga acgcagccgc atcgagcagg gccacgtcaa
     1321 gtaccgcgac aagctcaagg aagagggcaa gctcttcgtc cgcgaccgcc tgaagctgct
     1381 gctcgatccg gggagtgagt tccaggagga ttttctcttc gcgcgcaacc aggaggccga
     1441 cactcccgcc gacggggtgg tcactggggt ggggacggtc ggtggccgca ccgtatgcgt
     1501 gatggccaac gactacaccg tgaaggcggg gtcgtggggc gagaagaccg tccagaagat
     1561 cgtgcgcatc caggagcgcg cccagcggct gcaggttccg ctgctctacc tggtcgatgc
     1621 ggcgggcggt cgcatctcgg agcagatcaa gatcttcccc ggccgcttcc acgccggccg
     1681 catcttctac aacgaggtgc agctctccgg ggtggtgccg caggtgtgcg tactattcgg
     1741 cccctctccg gcgggctcgg cctatctccc ggccctcacc gactgcgtga tcatggtcga
     1801 cggcaaggcg agtctctacg tgggatcgcc ccgcatggtc gagatggcga tcggggagaa
     1861 gaccaccctc gaggagctgg gcggggcccg gatgcactgt gcggtctcgg gctgcggcga
     1921 catgctcgcc gcctcggacg aggaggccat cgagctctgc cgacgttatc tgagctacat
     1981 gcccggctcc ttccgggagc ggcccgcggt ggcggacgcg gcaccggcca agcccgggcg
     2041 ctcgatcgag gacatcgtcc cctacgacca gcgtaagtgg ttcgacatgt acgaggtcat
     2101 cgatcggatc gtggacgagg gctcgttctt cgagatcaag cgcctcttcg ccgccgagat
     2161 catcgtgggc ttcgcgcgcc tcgccggccg ggcggtgggc ctggtcgcca accagcccaa
     2221 ggtgaagggg ggagtgctga tggtcgactc ctccgacaag gcggcccgct tcatctcgct
     2281 gtgcaacgcc tacaacatcc cgctcgtgta cctggcggac gtctcgggct tcatggtggg
     2341 caccaaggtc gagcgcgccg ggatcatccg gcacggcgcc aagatggtgt tcgcgacctc
     2401 gcaggccacc gtgccgaaga tctgcgtggt
//
LOCUS       DATMCV010000892         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_386948, whole genome shotgun sequence.
ACCESSION   DATMCV010000892 DATMCV010000000
VERSION     DATMCV010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386948"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VNU03_20420"
     CDS             <1..1590
                     /locus_tag="VNU03_20420"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP76897.1"
                     /translation="APDVGGGFGAKLIVYPEDVLIPFLARRLARPVRWIEDRLEHMLA
                     ATQEREQEHEVTVGFDDAGRLLALRDRFVHDTGAYTPRGLVVPLLTASMLTGPYRIPA
                     VESSFVSRYTHRVPVTPYRGAGQPQAVFVIERVLDLVARETGRDRAQVRLANLVRAED
                     MPWDTGLPNYRGSGHVVLDSGDLPSVLRRALESAGYDARAAEAAAARAQGRLVGVGVA
                     CYVELTGVGPFESARVRIDAAGRIAAWSGVSTQGQGLETTLAQVAADELGVTPEDVAV
                     TMGDTAGVEHGTGSFASRAAVVGGNAIALAARDVYAKARLLATRALGLADADLEQTGA
                     AFADRRRPERRVTFAELARLAGAATAALGVEPGLESTRFFQPTDMTYSSGAHVALVEM
                     DPLSAAVRILGYWISHDSGRLINPLVVEGQLQGAVALGIGSALLEEIVYDEAGQLLAG
                     SYMDYLLPTATDVPTMVIDHLETLSPLNPLGLKGVGESGTLPVPAVLASAIEDAVGPR
                     GGRVTRMPLGPARLLDLLPPA"
     gene            complement(1614..2225)
                     /locus_tag="VNU03_20425"
     CDS             complement(1614..2225)
                     /locus_tag="VNU03_20425"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HWP76898.1"
                     /translation="MIFLPVAVPLLLLLLVVLLVVFVVLVELRILSYAYRKIGIAPRY
                     VFTILVLTLIGSHVNIPLYSVATRHAARATIVAVNVGGALIPALLSFYLFVRMPTRGR
                     MLIGTAVVALVMNQLARVVPGVGIVVPMLLPPLLAAAVALILAFRQAPPVAYVAGSMG
                     TLIGADLLNLGRIAEMGAPMLSIGGAGTFDGVFLTGILAGLLA"
     gene            2294..3067
                     /locus_tag="VNU03_20430"
     CDS             2294..3067
                     /locus_tag="VNU03_20430"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWP76899.1"
                     /translation="MTSHRIFEGVLQDLRLAIARGTIGPGRRLPAERALAEQFRVSRG
                     SVREAVRILELFGLVTVRRGRKGGVVTTPHCTEIARDSFASLQPLGRHGFQHSLEFRR
                     IVEPKVAELAALRATEDHLNTLHKSIRMLEEHPDSADVFVESNRLFHQTLALASGNPY
                     VQGFLPDFFATEVMAHAAGTAGPVQRSFARFFHGKITDAVARRDVKEAAVWMEAHLSQ
                     LESDFLRSAEVASVDDGPPAPGGGRRPRRSRARRARPTR"
     gene            3091..>3273
                     /locus_tag="VNU03_20435"
     CDS             3091..>3273
                     /locus_tag="VNU03_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76900.1"
                     /translation="MMPCRLKLFALAVLTLTLLGGPGSAAPAVAQVKIGVLMPLSGKG
                     ASYGAHQQVAIKMFQDK"
BASE COUNT          458 a         1208 c         1184 g          423 t
ORIGIN      
        1 gcccccgacg tgggcggcgg cttcggggcc aagctcatcg tttatccgga ggacgtcctg
       61 atcccgttcc tcgcgcggcg gctggcccgc ccggtgcgat ggatcgagga ccgcctcgag
      121 cacatgctgg ccgccaccca ggagcgggag caggagcacg aggtgacggt cggcttcgac
      181 gacgcgggcc ggctcctggc cctgcgtgac cgcttcgtcc acgacaccgg cgcctacacc
      241 ccgcgcggcc tcgtggttcc gctgctcact gcctcgatgt tgaccgggcc ctaccggatc
      301 cccgccgtcg agtcgagctt cgtgagccgc tacacccacc gcgtgccggt caccccgtac
      361 cgcggcgccg gccagccgca ggcggtgttc gtgatcgagc gggtgctcga cctggtggcg
      421 cgcgagaccg ggcgcgaccg cgcccaggtg cgcctcgcca acctggtccg cgccgaggac
      481 atgccgtggg acaccgggct gcccaactac cgcggcagcg gccacgtggt gctggacagc
      541 ggcgacctgc cgtcggtgct gcgccgggcg ctcgagagcg ccggctacga cgcgcgcgcc
      601 gccgaggccg ccgccgcgcg cgcccagggt cggctggtcg gcgtgggtgt cgcctgctac
      661 gtggagctga ccggcgtcgg ccccttcgag agcgcgcgcg tgcgcatcga cgcggccggg
      721 cgcatcgccg cgtggagcgg cgtctccacc cagggccagg ggctcgagac gacgctggcc
      781 caggtggcgg ccgacgagct gggggtcacg cccgaggacg tggcggtcac catgggcgat
      841 accgcggggg tcgagcacgg gacgggctcg ttcgcgagcc gcgccgcggt ggtcggcggc
      901 aacgcgatcg cgctcgccgc gcgcgacgtg tacgcgaagg cccgcctcct ggccacgcgc
      961 gccctcggac tcgccgacgc cgatctggag cagaccggcg cggccttcgc ggaccgccgg
     1021 cgccccgagc gccgcgtgac cttcgccgag ctggcccggc tggccggcgc ggcgaccgcc
     1081 gcgctcggcg tcgagcccgg gctcgagagc acgcggttct tccagcccac cgacatgacc
     1141 tactcgtcgg gtgcccacgt cgcgctggtg gagatggatc ccctctccgc ggcggtccgg
     1201 atcctcggct actggatcag ccatgactcg ggccgcttga tcaacccgtt ggtagtcgag
     1261 ggccagctcc agggcgcggt cgcgctcggc atcggcagcg cgctactcga ggagatcgtc
     1321 tacgacgagg ccggccagct cctggccggc agctacatgg actatctgct gcccaccgcc
     1381 accgacgtgc cgaccatggt catcgaccac ctcgagacgc tctcgccgct caacccgctg
     1441 gggctcaagg gcgtgggcga gtccggcacg ctgccggttc ccgcggtgct ggcctccgcc
     1501 atcgaggacg cggtcggccc gcgcggcggc cgcgtcaccc gcatgccgct cgggcccgcg
     1561 cgcctgctcg acctgctgcc gccggcctag cgccgcggcc gcgggccttc cgctcaggcg
     1621 agcaggccgg ccaggatccc ggtcaggaag acgccgtcga aggtgcccgc cccgccgatg
     1681 gacagcatcg gcgcgcccat ctccgcgatc cgccccaggt tgaggaggtc cgcgccgatc
     1741 agcgtcccca tgctgcccgc gacgtaggcg acgggcggcg cttggcggaa cgcgaggatc
     1801 agcgccaccg ccgcggccag cagcgggggc aggagcatcg gcaccacgat accgacgccg
     1861 gggaccacgc gggcgagctg gttcatcacc agcgcgacca ccgcggtccc gatcagcatg
     1921 cggccgcgcg tgggcatccg gacgaagagg tagaacgaga gcagcgccgg gatgagcgca
     1981 ccgcccacgt tgaccgccac gatggtggct ctcgcggcat gccgggtggc cacggagtag
     2041 agcgggatgt tgacgtggct cccgatcagg gtcagcacca ggatcgtgaa gacgtagcgg
     2101 ggcgcgatgc cgatcttccg gtaggcgtag gagaggatcc gcagctcgac caggaccacg
     2161 aagaccacca gcagcacgac gagcagcagc aacaacaggg ggaccgccac cgggagaaag
     2221 atcatcggcc gcagcataga ccggcggggg cgccttgaca atggtcggcc cagtagcgta
     2281 ttgggtctga ccaatgacct cccaccgcat cttcgagggc gtgctccagg acctgcgcct
     2341 cgccatcgcg cgagggacga tcggcccggg gcggcggctg cccgcggagc gggcgctggc
     2401 cgagcagttc cgggtgagcc ggggctcggt gcgggaggcg gtccggatcc tcgagctgtt
     2461 cggcctcgtg accgtgcggc ggggccgcaa gggcggggtg gtcaccaccc cccactgcac
     2521 cgagatcgcg cgggactcgt tcgcctcgct ccagcccctc ggccggcacg gcttccagca
     2581 ctcgctcgag ttccggcgca tcgtcgagcc gaaggtggcg gagctggccg ccctgcgggc
     2641 gaccgaggac catctgaaca cgctgcacaa gagcatccgg atgctcgagg agcaccccga
     2701 ctcggccgac gtcttcgtcg agagcaaccg gctcttccac cagacgctgg cgctggcgag
     2761 cggcaacccg tacgtccagg gcttcctgcc cgacttcttc gccaccgagg tcatggccca
     2821 cgcggccggg accgcgggcc cggtgcagcg atcgttcgcg cggttcttcc acggcaagat
     2881 caccgacgcg gtggctcggc gcgacgtcaa ggaggccgcg gtgtggatgg aggcgcatct
     2941 ctcccagctc gagagcgact tcctgcgcag cgcggaggtc gcgagcgtgg acgacggccc
     3001 gccggcgccc ggtggcggcc gtcgcccccg ccgctcccgg gcccgccgcg cccgcccgac
     3061 ccgatgaccc ccaaccagga ggacgcacgc atgatgccct gcaggctcaa gctcttcgcg
     3121 ctggcggtgc tgacgctcac cctgctcggc ggtccgggat cggccgctcc ggccgtggcc
     3181 caggtgaaga tcggcgtgct gatgccgctc tccggcaagg gcgcctccta cggcgcccat
     3241 cagcaggtgg cgatcaagat gttccaggac aag
//
LOCUS       DATMCV010000893         2050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_712277, whole genome shotgun sequence.
ACCESSION   DATMCV010000893 DATMCV010000000
VERSION     DATMCV010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712277"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VNU03_20440"
     CDS             complement(<1..512)
                     /locus_tag="VNU03_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWP76901.1"
                     /translation="MYERRRNIVVRRDGRRVNVARFRGQLFVCATGCCCGRTEDGFAP
                     VPTETFHREWERRRLRNVIHLTIGGCLGPCALANVVLLLFDGQTQWFHSIDSEAQALA
                     LYDHVERMLEADGCLPPPAGLAPYHFTASASQPRPDGQPVDDARLWRAARGHACEAPA
                     SGVTADGRALS"
     gene            complement(529..1131)
                     /gene="nthA"
                     /locus_tag="VNU03_20445"
     CDS             complement(529..1131)
                     /gene="nthA"
                     /locus_tag="VNU03_20445"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HWP76902.1"
                     /translation="MGKNHPNVDPYVVSRVRALESLLLEKGILAPDAVDRVIQRYEAD
                     TGPMVGARAVARAWTDPEYKRLLLDDPRTALARFELDYQMLMVVENTDALHNLVVCTL
                     CSCYPWPVLGLPPTWYKMPAYRSRAVSEPRALLGEMGLAIDPRREIRVWDSSAEMRYM
                     VLPQRPAGTDGLGEAELVPLITRDALIGVADVRPPAPAAR"
     gene            complement(1138..1818)
                     /gene="nthB"
                     /locus_tag="VNU03_20450"
     CDS             complement(1138..1818)
                     /gene="nthB"
                     /locus_tag="VNU03_20450"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HWP76903.1"
                     /translation="MDGVHDLGGMHGFGPVEREENEPVFHAAWEAAMLAIQRAGGARG
                     YYNIDEFRHGIERMAPSHYLRATYYEKWLDGVTRVLVEKGVLDPAELAARVAYFEQRP
                     DASATEPLAAPLPPRPPANSKWAQDVIRETGAAPRFADGDPVRTRNVHPPGHTRLPRY
                     ARGKHGVIHRCHGIHVFPDTHAHGGGEQPQPLYSVRFDARELWGESAEPNQAVHIDLW
                     ESYLLPGS"
BASE COUNT          276 a          721 c          773 g          280 t
ORIGIN      
        1 gagagggccc gcccgtcggc agtgacgccg gaggcgggcg cctcgcacgc gtgcccgcgg
       61 gcggcccgcc agagccgcgc gtcgtccacc ggctgcccgt ccgggcgcgg ctgcgaggcc
      121 gacgcggtga agtgatacgg ggcgagcccg gccggcggag gcaggcaccc gtcggcttcc
      181 agcatgcgct ccacgtgatc gtagagcgcg agggcctgcg cctcggagtc gatcgagtgg
      241 aaccactggg tctggccgtc gaagagcagg agcaccacgt tggcgagcgc gcacgggccg
      301 aggcagccgc cgatcgtgag gtggatcacg ttgcggaggc gccgccgctc ccactcgcgg
      361 tggaaggtct cggtgggcac cggggcgaag ccgtcctcgg tgcggccgca gcagcagccg
      421 gtggcgcaca cgaagagctg cccgcggaag cgggccacgt tgacgcggcg gccgtcacgc
      481 cgcaccacga tgttgcgtcg ccgctcgtac acgggaggtc gcgccgtctc agcgggcggc
      541 gggcgcgggc ggccgcacgt cggccacccc gatcagcgcg tcgcgcgtga tcaggggcac
      601 cagctccgcc tcgccgagcc cgtcggtgcc ggccggccgc tgcggcagca ccatgtagcg
      661 catctccgcg ctcgagtccc acacccggat ctcgcgccgc ggatcgatcg cgagccccat
      721 ctcgcccagc agtgcgcgcg gctcgctcac cgcgcgcgag cggtaggccg gcatcttgta
      781 ccaggtcggg ggcagcccga gcaccggcca cgggtagcag gagcagagcg tgcagaccac
      841 caggttgtgc agcgcgtcgg tgttctcgac caccatcagc atctggtagt cgagctcgaa
      901 gcgggcgagc gcggtccgcg ggtcgtcgag caggaggcgc ttgtactcgg gatcggtcca
      961 ggcccgggcc accgcccgcg cgcccaccat cgggccggtg tccgcctcgt agcgctggat
     1021 cacccggtcg accgcgtcgg gcgccaggat gcccttctcg agcagcagcg actcgagcgc
     1081 ccgcacccgg gagaccacgt agggatcgac gttggggtgg ttcttgccca tgggggatca
     1141 gctcccgggg aggagatagc tctcccacag atcgatgtgc accgcctggt tcggctccgc
     1201 cgactcgccc cacagctcgc gggcgtcgaa gcgcacgctg tagagcggct ggggctgctc
     1261 gcccccgccg tgggcgtggg tgtcgggaaa cacgtggatg ccgtgacagc ggtggatgac
     1321 gccatgcttg ccccgcgcgt agcgtggcag ccgcgtgtgc ccgggcgggt ggacgttgcg
     1381 tgtgcgcacc gggtcaccgt ccgcgaagcg cggtgccgcg ccggtctcgc ggatcacgtc
     1441 ctgcgcccac ttggagttgg cgggcggccg cggcgggagc ggcgcggcca ggggctcggt
     1501 cgccgacgcg tcgggccgct gctcgaagta ggccacccgc gcggccagct cggcgggatc
     1561 cagcaccccc ttctccacga gcacgcgggt cacgccgtcg agccatttct cgtagtaggt
     1621 cgcgcgcagg tagtgggacg gggccatgcg ctcgatgccg tgacggaact cgtcgatgtt
     1681 gtagtagccg cgcgcgccgc ccgcgcgctg gatcgcgagc atcgccgctt cccacgccgc
     1741 gtggaagacg ggctcgttct cctcccgctc caccggcccg aagccgtgca tgccgcccag
     1801 gtcgtggact ccgtccatgc ccgcgattct atcggctggg gtcaggtctt acaatccgac
     1861 aatgtcggcc aacattgtca gccaacattg tcagaatgta ggacctgacc ccatcagtgc
     1921 gtccgcggcg gcgtgaggtc ggccggcggg ggctcggcgg gaacgcccgc gtggcgggcg
     1981 cgcaggtcca tccagaggcg gaggcgggac cagtcgggcg tgtccacgta gcggccgccg
     2041 cgggcggggt
//
LOCUS       DATMCV010000894         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_249473, whole genome shotgun sequence.
ACCESSION   DATMCV010000894 DATMCV010000000
VERSION     DATMCV010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249473"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VNU03_20455"
     CDS             <1..291
                     /locus_tag="VNU03_20455"
                     /inference="COORDINATES: protein motif:HMM:NF021883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWP76904.1"
                     /translation="RENMERSFGLLYSQRVLLKLTDKGLARQKAYEMVQRHAMTAWRD
                     RTAFRDLLASDPEVMAQLTRPELDECFDPAWYLRNVAAIYRRIGLPAEGGSA"
     gene            288..539
                     /gene="purS"
                     /locus_tag="VNU03_20460"
     CDS             288..539
                     /gene="purS"
                     /locus_tag="VNU03_20460"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWP76905.1"
                     /translation="MKARVLVRLKPSILDAQGATVQRALAGLGFAEVQDVRVGKVVDV
                     ELTGLAPDEARRRVDEMCARLLCNPVIEDFTVEMLGDGR"
     gene            641..1354
                     /gene="purQ"
                     /locus_tag="VNU03_20465"
     CDS             641..1354
                     /gene="purQ"
                     /locus_tag="VNU03_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWP76906.1"
                     /translation="MNFGVVIFPGTWSDCDFHYVIGEVLHQPVRYVWHRDANLADFDC
                     LILPGGFSYGDYLRAGAVAGRSPVVEALPEFTARGGLVLGSCNGFQILCEARLLPGAL
                     MRNECLQYRCQSTHLIVENADTAFTRAMRPGQVLKMPISHGEGKYHADAETIRSLKAK
                     NQIVFRYCTENGRVVKEANPNGSMDNIAGIINEEGTVLGLMPHPERAAESAVGGTDGT
                     LIFHSILGTLVEDGSFLKR"
     gene            1384..>2147
                     /locus_tag="VNU03_20470"
     CDS             1384..>2147
                     /locus_tag="VNU03_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HWP76907.1"
                     /translation="MTAGEPKVTLDLAQSHGLTAEEYDRIIRRLEREPTFTELGLFSA
                     LWSEHCAYKHSRVFLRGLPTQAPHVLQGPGENAGIVDLGGDLALTFKIESHNHPSFIE
                     PFQGAATGVGGILRDIFTMGARPIAVLDSLRFGDPADPRTRRLIEGVVSGIGWYGNCF
                     GVPNLGGEVGFAAEYAGNPLVNAMAVGLVSKRRIFRARADGVGNPVFYVGAKTGRDGI
                     HGATMASATFDETAEERRPTVQVGDPFTEKLLLEACL"
BASE COUNT          330 a          749 c          733 g          335 t
ORIGIN      
        1 cgggagaaca tggagcggag cttcgggctc ctctactccc agcgggtgct cctcaagctc
       61 accgacaagg gcctcgcccg tcagaaggcc tacgagatgg tgcagcgcca cgcgatgacc
      121 gcgtggcgcg atcgcaccgc cttccgggac ctgctggcct ccgaccccga ggtgatggcc
      181 cagctgaccc gccccgagct cgacgagtgc ttcgatcccg cgtggtacct gcgcaacgtc
      241 gcggccatct accggcggat cggcctgccc gcggagggag gctctgcgtg aaggcgcgcg
      301 tcctcgtccg cctcaagccg tccatcctgg acgcccaggg cgctaccgtg cagcgggcgc
      361 tggccgggct gggcttcgcg gaggtgcagg acgtgcgcgt cggcaaggtg gtggacgtcg
      421 agctgaccgg tctcgccccc gacgaggccc gtcggcgggt cgacgagatg tgcgcgcgtc
      481 tgctctgcaa cccggtgatc gaggacttca cggtggagat gctcggcgac ggtcgctagc
      541 cgcgggaatc gacctctttt gctccgcgag cctcttgtca gatcgtagac ggcgggttag
      601 agtgacgaag tagcaaccac aaggaggaac acacggtccc atgaactttg gcgtggtgat
      661 cttcccgggc acgtggagcg attgcgattt tcactacgtc atcggcgagg tgctgcacca
      721 gccggtccgc tacgtctggc atcgggacgc caacctcgcc gacttcgact gcctgatcct
      781 gcccggtggg ttctcctacg gtgactatct ccgggccggc gcggtcgcgg gccgctcccc
      841 ggtggtcgag gccctgcccg agttcaccgc gcgcggcggc ctcgtgctcg ggtcgtgcaa
      901 cggcttccag atcctctgcg aggcccggct cctccccggg gccctgatgc gcaacgagtg
      961 cctgcagtac cgctgccagt cgacccacct gatcgtcgag aacgcggaca ccgccttcac
     1021 ccgtgcgatg cgtcccggcc aggtgctcaa gatgcccatc tcgcacggcg agggcaagta
     1081 ccacgccgac gcggagacga tccgctcgct caaggccaag aaccagatcg tcttccgcta
     1141 ctgcaccgag aacggccgcg tggtgaagga agccaacccc aacgggtcga tggacaacat
     1201 cgccgggatc atcaacgagg aaggcaccgt gctcggcctg atgccgcatc cggagcgggc
     1261 cgccgagtcc gcggtggggg gtaccgacgg taccctcatc ttccactcga tcctcggcac
     1321 cctcgtcgaa gacggctcgt tcctcaagcg gtaggtcacg cgtgcgaacc gggtgggggc
     1381 ggggtgaccg cgggcgagcc taaggtcacg ctggacctgg cccagagcca cggcctcacg
     1441 gccgaagagt acgatcgcat catccggcgc ctcgagcgcg agcccacgtt caccgagctg
     1501 ggactcttct ccgccctctg gtccgagcac tgcgcctaca agcactcccg ggtgttcctg
     1561 cgcgggctgc ccacccaggc gccgcacgtg ctccaggggc cgggcgagaa cgcgggcatc
     1621 gtggatctcg gcggggacct cgccctgacc ttcaagatcg agagccacaa tcacccgtcc
     1681 ttcatcgagc ccttccaggg ggcggccacc ggggtgggcg gcatcctgcg cgacatcttc
     1741 acgatgggcg cccgcccgat cgcggtcctc gactcgctcc gcttcggcga tccggccgac
     1801 ccgcggacac ggcggctgat cgagggcgtg gtgtcgggca tcggctggta cggcaactgc
     1861 ttcggcgtgc cgaacctggg cggcgaggtg ggcttcgcgg ccgagtacgc gggtaacccg
     1921 ctggtcaacg cgatggcggt cgggctcgtc agcaagcggc ggatcttccg ggcccgcgcc
     1981 gacggggtgg gcaacccggt cttctacgtc ggggccaaga ccgggcgcga tggcatccac
     2041 ggcgccacca tggcctcggc cacgttcgac gagaccgcgg aggagcgccg gccgaccgtg
     2101 caggtagggg atccgttcac cgagaagctg ctcctcgagg cctgcct
//
LOCUS       DATMCV010000895         4942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_583526, whole genome shotgun sequence.
ACCESSION   DATMCV010000895 DATMCV010000000
VERSION     DATMCV010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583526"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1410
                     /locus_tag="VNU03_20475"
     CDS             46..1410
                     /locus_tag="VNU03_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWP76908.1"
                     /translation="MQTKTGLKQSICAAIDRNAERIITLGERIRRHPELGFKEFKTAR
                     LAEETFSELGLKPRGGLALTGVRAEAAGAKDGPTFALLGELDGLVVAGHPIADPETGA
                     AHACGHNAQMAGLLGAAMGLLEARAFEHLAGRVVFFAVPAEEYGDVEWRVQQARAGRI
                     EFLGGKPELLRLGHFDDVDLAMMIHTTSQPDMKKAGVAASNNGCIVKTVRYIGRASHA
                     GGAPHMGINALYAANIGLAAINAIRETFRDEDSIRVHPIITHGGSQVNVIPGEVRLET
                     YVRGKTVEAILEANWKVDRALRAGAMALGAQVEIETLPGYLPLFNHMGMAGMFKDNAA
                     ALLGADQVTESGHRSGSTDMGDISHVMPTLHPYMGGAIGGGHGADFAIADPKVAYVEP
                     AKQLALMAVDLLWGDADGARDILKTWKPRMTKDAYLSFQRSVSRTELYEPKDLPLPSG
                     ERAG"
     gene            1407..2234
                     /locus_tag="VNU03_20480"
     CDS             1407..2234
                     /locus_tag="VNU03_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HWP76909.1"
                     /translation="MRGNPRAMYGKSPVTFPQRIVCLSAEHVEICYALGAGDRVVGVP
                     GTAHRPPEARDKPKVGGFTTFKVDRILALEPDLVLAFSDLQADVSAELIRAGRPVFCT
                     NPRSMDEILQSILMVGGVLGVDAAAHALVADMHDEINQVREFSTMWPDRPRVYFEEWH
                     EPLISGIRWVSEVIELAGGRDVFAELRDHRSARERVVDAAEVARRDPQIILVSWCGKP
                     ADLDLVRGRPGWDRISAVREGRVHEVDAATLLSPGPALLTGLRTVHELVQQALRDRD"
     gene            2394..3830
                     /gene="tldD"
                     /locus_tag="VNU03_20485"
     CDS             2394..3830
                     /gene="tldD"
                     /locus_tag="VNU03_20485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HWP76910.1"
                     /translation="MLARPERFFADRFGLNAGTMERLLGSTLAGRVDHADLYLEYRVS
                     EELVLEEGAVKKAARHVSQGAGIRAQSGERTGYAHTDDLALPNLEEAARQARAIADRS
                     SESAVVAVGGDGPPHDLYSLAEPPIAADLDRKIKLLGRMDAAARALDPRVRQVIATLG
                     SEDVVVMIATPSGWTVGDVRPLTRLNITVIVEDGAKREIGSFGGGGRVAFDFFLEDDR
                     WQRFASEAVRQALMKLGAVDAPAGEMTVVLGPGWPGILLHEAVGHGLEGDFNRKKTSA
                     FSGRLGQPVASELVTVVDDGTIANRRGSLNVDDEGVPTRRNVLIEKGILRGYMQDRLN
                     SRLMGMEVTGNGRRESYAHPPMPRMTNTFMLAGQDEPADIIRSVPKGLYAVNFGGGQV
                     DITSGRFVFSASEAYLIEDGRVTKPVKGATLIGNGPEALTQVSRVGHDLKLDEGVGTC
                     GKDGQSVPVGVGLPTIRIDRMTVGGTQV"
     gene            3839..>4942
                     /locus_tag="VNU03_20490"
     CDS             3839..>4942
                     /locus_tag="VNU03_20490"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWP76911.1"
                     /translation="MQDLLADLLKQATRKGATEADAFVVEDQSFSAQVRLGQVDTVKH
                     AREQHMALRVFVGKSVAAASTSDLSPQAMTRLVDEAVSLARVTSPDELSGLPDAALLA
                     REIPDLDLRDPRGHDLSPEEKIDLARRCEAAALAADPRITNSEGGDFGDRRARYAYAT
                     SHGFAGEYATSSFSLAVSPVATRDAEMQRDSWYHVTRRRARLDDPESIGRIAAQRAVR
                     RLGARRVKTAEVPVVFDPDMAASLVRHIAGAASGPALYRRASFLVGKLGEQIASPAVT
                     IVDDGTVPGALGSRPFDGEGLPVRRTVVVDRGTLRSYLLDTYSGRKLGLPSTHHGARD
                     GSGVSVSTTNLYLAAGDRDPGDLIRSVKNGLYVT"
BASE COUNT          760 a         1764 c         1755 g          663 t
ORIGIN      
        1 atcaccccgt tccttcccca gcgcttcgtg tcataggaga ttcccgtgca aacaaaaacc
       61 ggcctcaagc agtccatctg cgccgccatc gaccgcaacg cggagcgcat catcaccctc
      121 ggcgagcgca tccggcgcca tcccgagctc ggcttcaagg agttcaagac cgcgcgcctg
      181 gccgaggaga cgttcagcga gctgggcttg aagccccgag gcgggctcgc gctgaccggg
      241 gtacgcgcgg aggcggcggg ggccaaggac ggcccgacct tcgccctgct cggcgagctg
      301 gacggcctgg tggtggccgg ccacccgatc gccgatccgg agacgggggc cgcgcacgcg
      361 tgcggccaca acgcgcagat ggcggggctg ctcggcgccg caatgggcct gctcgaggcg
      421 cgcgccttcg agcacctggc cggccgggtg gtcttcttcg cggtgcccgc cgaggagtac
      481 ggtgacgtgg agtggcgcgt gcagcaggcg cgggcggggc gcatcgagtt cctcggcggc
      541 aagccggagc tgctgcgcct cggccacttc gacgacgtcg acctcgcgat gatgatccac
      601 accacctcgc agcccgacat gaagaaggcg ggggtggccg cctccaacaa cggctgcatc
      661 gtgaagacgg tgcgctacat cgggcgcgcc tcgcacgcgg gcggggcgcc gcacatgggg
      721 atcaacgcgc tctacgcggc caacatcggc ctcgccgcga tcaacgcgat ccgggagacc
      781 ttccgcgacg aggactcgat ccgggtccac ccgatcatca cccacggggg cagccaggtg
      841 aacgtgatcc cgggcgaggt gcgcctcgag acctacgtgc gcggcaagac cgtcgaggcg
      901 atcctcgagg cgaactggaa ggtggaccgc gcgctccggg ccggcgccat ggcgctgggc
      961 gcgcaggtcg agatcgagac gctgcccggc tacctcccgc tcttcaacca catgggcatg
     1021 gccgggatgt tcaaggacaa cgcggccgcc ctgctgggcg ccgaccaggt caccgagtcc
     1081 ggccatcgca gcggctccac tgacatgggc gatatctccc acgtgatgcc gacattgcat
     1141 ccctacatgg gcggggcgat cggcggcggg cacggcgccg acttcgcgat cgccgatccg
     1201 aaggtggcat atgtggagcc ggccaagcag ctcgccctga tggcggtcga cctgctgtgg
     1261 ggcgacgccg acggagcgcg cgacatcctc aagacatgga agccgcgcat gaccaaggac
     1321 gcgtacctct ccttccagcg ctccgtctcg cggaccgagc tctacgagcc aaaggacctc
     1381 cctctcccct ccggggagag ggcagggtga ggggaaaccc gcgcgcaatg tacggcaagt
     1441 caccggtaac tttcccgcag cgcatcgtct gcctgagcgc ggagcacgtg gagatctgct
     1501 acgcgctcgg agcgggggac cgcgtggtcg gcgtgcccgg caccgcgcac cgaccgcccg
     1561 aggcccgcga caagcccaag gtcggcggct tcacgacctt caaggtcgac cggatcctcg
     1621 ccctcgagcc cgacctggtc ctggccttct ccgacctcca ggccgacgtc tccgccgagc
     1681 tgatccgggc cggccggccc gtcttctgca ccaatccacg ctcgatggac gagatcctcc
     1741 agtccatcct gatggtgggc ggcgtcctcg gggtcgacgc cgcggcgcac gccctcgtcg
     1801 ccgacatgca cgacgagatc aaccaggtgc gcgagttctc caccatgtgg cccgaccggc
     1861 cgcgggtgta cttcgaggag tggcacgagc cgctcatctc cggcatccgg tgggtgtccg
     1921 aggtcatcga gctggccggc ggccgcgacg tgttcgcaga gctgcgcgat caccggagcg
     1981 cgcgggagcg ggtggtggac gcagccgagg tggcgcggcg cgatccccag atcatcctgg
     2041 tgtcctggtg cggcaagccc gccgacctcg acctggtccg cggccggccc ggctgggacc
     2101 gcatcagcgc ggtgcgcgag ggccgcgtgc acgaagtcga cgcggccacg ctgctcagtc
     2161 ccggccccgc gctgctcacc gggctgcgaa cggtgcacga gctggtgcag caggccctcc
     2221 gcgaccgcga ctgagtctcg acacgatcct gagtgccgcc acggcaaagc gtggacatat
     2281 ctgcgataat caaaagtgac gcggagcacg cgtggccacg tactaaacat ggcggcccag
     2341 cctgagcccg atgctaggat gaaccgcttt cgagagactc ccaggagaga gcaatgctag
     2401 cgcgcccgga gcgcttcttc gctgatcggt tcggattgaa cgccgggacg atggagcggc
     2461 tgctgggctc gaccctcgcg ggtcgcgtcg accacgccga cctctacctc gagtaccgcg
     2521 tcagcgagga gctggtgctg gaggaaggcg cggtcaagaa ggcggcccgc cacgtgagcc
     2581 agggggcggg catccgggcc cagtcgggtg agcgtaccgg ctacgcccac accgacgacc
     2641 tcgcgctgcc caatctcgag gaggccgcgc ggcaggcgcg ggccatcgcc gaccgctcct
     2701 ccgagtccgc agtggtcgcg gtgggcggcg acgggccccc gcacgatctc tacagcctcg
     2761 ccgagccgcc gatcgcggcg gacctcgatc ggaagatcaa gctgctcggg cgcatggacg
     2821 cggcggcgcg cgcgctcgac ccgcgggtgc gccaggtcat cgcgaccctc ggcagcgagg
     2881 acgtggtggt catgatcgcg accccgtccg gctggacggt gggagacgtg cgcccgctca
     2941 cccggctcaa catcacggtg atcgtcgagg acggggcgaa gcgggagatc ggcagcttcg
     3001 gcggcggcgg ccgcgtggcg ttcgacttct tcctggaaga cgaccgctgg cagcgcttcg
     3061 cctccgaggc ggtacggcag gcgctcatga agctcggggc ggtggacgcc cccgccggcg
     3121 agatgacggt ggtgctgggg cccggctggc ccggcatcct gctccacgag gcggtcgggc
     3181 acgggctcga gggcgacttc aaccgcaaga agacctccgc attctccggg cggctcggcc
     3241 agccggtcgc ctcggagctg gtgacggtgg tcgacgacgg cacgatcgcc aaccggcgcg
     3301 gctcgctcaa cgtggacgac gagggcgtgc cgacccgccg caacgtcctc atcgagaagg
     3361 gcatcctgcg cggctacatg caggaccggc tcaactcgcg gctcatgggc atggaggtca
     3421 ccggcaacgg gcggcgcgag tcgtacgcgc acccgccgat gccgcgcatg accaacacct
     3481 tcatgctcgc ggggcaggac gagccggccg acatcatccg gagcgtgccg aaggggctct
     3541 acgcggtgaa cttcggcggc ggccaggtcg acatcacgag cggccgcttc gtgttctcgg
     3601 ccagcgaggc ctacctgatc gaggacggcc gcgtgaccaa gccggtgaag ggcgcgaccc
     3661 tgatcggcaa cgggccggag gcgctcaccc aggtgagccg cgtgggccac gatctcaagc
     3721 tcgacgaagg cgtggggacc tgcggcaagg acggccagtc ggtgccggtg ggggtggggc
     3781 tgcccaccat ccgcatcgat cggatgaccg tcggcgggac ccaggtctga ccggccgcat
     3841 gcaggatctc ctcgccgatc tcctgaagca ggcgacccgc aagggggcca ccgaggccga
     3901 cgccttcgtg gtggaagacc agtcgttctc ggcccaggtc cgcctgggcc aggtggatac
     3961 cgtcaagcac gcccgcgagc agcacatggc gctgcgggtg ttcgtgggca aatcggtggc
     4021 ggccgcctcc acctcggatc tgtcgccgca ggccatgacc cgcctggtcg acgaggcggt
     4081 gtcgctcgcc cgggtcacca gccccgacga gctgtccggc ctgccggacg cggcgctgct
     4141 cgcgcgggag atcccggatc tcgatctgcg cgacccgcgc ggccacgatc tgtcgcccga
     4201 ggagaagatc gacctggccc ggcgctgcga ggcggccgcg ctcgcggcgg atccgcgcat
     4261 caccaactcg gagggcggcg acttcggcga ccgccgcgcc cgctacgcct acgccaccag
     4321 ccacggcttc gcgggcgagt acgcgacgtc gtccttcagc ctcgcggtgt ccccggtggc
     4381 cacccgcgac gcggagatgc agcgcgactc gtggtaccac gtgacccgcc ggcgcgcccg
     4441 tctcgacgat cccgagtcga tcggccgcat cgcggcgcag cgcgcggtgc gccgcctggg
     4501 cgcgcgccgg gtgaagaccg ccgaggtgcc ggtggtcttc gatcccgaca tggccgcctc
     4561 gctggtgcgg cacatcgcgg gcgcggcgtc ggggcccgcg ctctaccgcc gcgcgtcctt
     4621 cctggtgggc aagctcggcg agcagatcgc gtcgcccgcg gtcaccatcg tcgacgacgg
     4681 caccgtgccc ggcgccctcg gctcgcgacc cttcgacggg gagggcctcc cggtccgccg
     4741 caccgtggtg gtggaccggg gcacgctgcg ctcctacctg ctcgacacct acagcggccg
     4801 caagctcggg ctgccgtcca cgcaccacgg ggcccgcgac ggcagcgggg tgagcgtgtc
     4861 gaccaccaac ctctacctcg ccgcgggcga ccgcgacccg ggcgacctga tccgcagcgt
     4921 gaaaaacggc ctctacgtca cc
//
LOCUS       DATMCV010000896         7293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_712478, whole genome shotgun sequence.
ACCESSION   DATMCV010000896 DATMCV010000000
VERSION     DATMCV010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712478"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1122)
                     /locus_tag="VNU03_20495"
     CDS             complement(298..1122)
                     /locus_tag="VNU03_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76912.1"
                     /translation="MDLRAEGLGLGSIQEINALPREVAEALYARLVPDELLDRFGIDP
                     ATLCDRDGARLVRVTAPPDKPWARVEVRSSTEDRDPALLVDVEMSPLSVPELAFVQIT
                     DPAGPRFAIDRDPDGRDTLFGTASRNVVEEARAMQDGLAPGQVRRGLRLLSRVLDSME
                     SFCTLIGKEIYLIEPLFYHSAILYERRGCGYLMGRDVMEEIHAGFSEGGSLLTALDGS
                     SHFRRPEAARTVRGRSWALHDGVIEGLWGGAGVGAVKMYKAAGRPAGIDTFPGAPY"
     gene            complement(1139..1762)
                     /locus_tag="VNU03_20500"
     CDS             complement(1139..1762)
                     /locus_tag="VNU03_20500"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWP76913.1"
                     /translation="MRITGVIQAGGRSVRMGGRPKALMELGGRRIIDRVADVVRAVAD
                     DVLLVTNTPELYASLGLPMVPDAFPDHGSLGGIYSGLRAARGDVAFTVACDMPFLMVE
                     VARLVTARAAEADVVVPRVGAQWETLHACYGKACLAPIETRLRAGRLKIVGFYEDVRV
                     LAIGEDEVARHRAPEIVFMNVNTPEDLEAARGLLPTLEPGPAPAGPR"
     gene            complement(1753..2394)
                     /locus_tag="VNU03_20505"
     CDS             complement(1753..2394)
                     /locus_tag="VNU03_20505"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWP76914.1"
                     /translation="MSAPPIRTRIRAVDPEQPDVRAIAAAAAVVEAGGLVAFPTESFY
                     GLGADALDSDAVARVFEVKGRPEHKALLVLVDSIDMAAGLAARISDGARALMARYWPG
                     PLTLVLEAADRVPAGLTGGGSTIGVRMPGHAVARALVHSAGRPITAPSANPSEAPPAL
                     TAAAVREYFEGRVELILDGGPTAGGAGSTVADCTVWPPRILRQGPVIVEAACG"
     gene            complement(2391..3671)
                     /gene="purD"
                     /locus_tag="VNU03_20510"
     CDS             complement(2391..3671)
                     /gene="purD"
                     /locus_tag="VNU03_20510"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWP76915.1"
                     /translation="MTDVLLVGGGGREHALAWKLAQSPGLGRMVAAPGNPGIAALARC
                     VPIKDTAIDQIVALAQQERPDLVVVGPETPLALGLADRLRAAGLAVFGGSAAAARLES
                     SKAFAKDLMARHRIPTARFGTFRDAAAARRYCRELGAPLVVKADGLAAGKGVVVCGSL
                     EEADRAVAQCLEARAFGDSGLTVVIEEFLVGEEASFFALSDGTGVLPLVAAQDHKTVH
                     DGDRGPNTGGMGAYSPAPVMDAGMQERVMREIVRPTIAAMAEEGAPYTGVLFVGLMIT
                     RDGPKVIEFNCRFGDPECQAILPRLEDDLLALLLATATGKGLPPALAFSPRSSVCVVM
                     TSAGYPGAYETGRAITGVEAAAGLPGVNVFHAGTALAGGALVTAGGRVLGVQALGADV
                     AAAIRTAYAAVERIRFDGAHYRRDIGHHALRRLG"
     gene            complement(3668..4564)
                     /locus_tag="VNU03_20515"
     CDS             complement(3668..4564)
                     /locus_tag="VNU03_20515"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP76916.1"
                     /translation="MFSLRELTRLLRLSPKRRSQLRRLGLLRDDPRGVRFRELVSARA
                     AAQLLESGASVRQVRAALDGARRLVPDVQTPLAELRLVVHDRRVVVEQDRLRLDPGTG
                     QALLDFAAGDLERETREVLLTGMVRPLIPPADAAEVWFQRASEWDGDPERWESAVDAY
                     ERVLDMDPGYAAAWNNLGLLHHRMGQYERAGECYRAALDADDSCCQAGFNLGSLHEDL
                     GDFPVAVTWYRRALELDADYADAHFNLAGVLAKMGRSESAAVHWRRYLELDLGSPWAK
                     IARAHLDGEAGGPDDAAPESDE"
     gene            complement(4581..6158)
                     /gene="purH"
                     /locus_tag="VNU03_20520"
     CDS             complement(4581..6158)
                     /gene="purH"
                     /locus_tag="VNU03_20520"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWP76917.1"
                     /translation="MTKVGRALISVHDKTGVVDFARGLVALGAEIISTGGTARLLRES
                     GIAVVDVAEVTGFPEMLDGRVKTLHPKVHGGLLARRELPEHMAALERHGIRPIDLVAV
                     TLYPFEQTVARTGVTLEEAIENIDIGGPSMIRGAAKNHAHVAVLTDPAQYGPVLDELR
                     KADGTLSDETRFRLAAEAFRRTAQYDAAIAAYLRDPAAAGRPAAAGFPERLAVDGVRV
                     QSLRYGENPHQAAAFYRPTAGALGLSAAAQLHGPELSYNNLLDWSAALGLLLEFDDPA
                     AVVIKHTNPCGVALGAGAGEAVRRAKACDPVSIYGGIVGLNRTVDLEVVKELSGILLE
                     ILFAPAYEPDALEEIRRTKKKCRVFQLPCARRDYPARAPELRSVWGGVLLQEADLVDL
                     DEGNLKVVSRRAPSAAELSALRFAWRVGKHAKSNAIVLATADQVVGVGAGQMNRVDSA
                     RLAVMRAREVGLETRGSVCASDAFFPFRDGLDVVAKAGATAVIHPGGSLRDEEVVAAA
                     DQHDMAMVVCGIRHFKH"
     gene            complement(6155..6775)
                     /gene="purN"
                     /locus_tag="VNU03_20525"
     CDS             complement(6155..6775)
                     /gene="purN"
                     /locus_tag="VNU03_20525"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWP76918.1"
                     /translation="MSEPLRVGVLASGRGSNFRALVLAAESGRMPAAVVVLVADRAGA
                     PVLDIAREHRIEAVVVDPRQHPSREAHEKAVIGVLEERRVGLVCLAGYMRLLTAGFVA
                     HFAGRLLNIHPSLLPAFPGLHPHRQALAHGVRVSGATVHFVDEGVDSGPVVLQAAVPV
                     QPDDTEESLAARILVEEHRIYPEAVRLFAEGRLEIHGRRVTIRETP"
     gene            complement(6898..>7293)
                     /locus_tag="VNU03_20530"
     CDS             complement(6898..>7293)
                     /locus_tag="VNU03_20530"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWP76919.1"
                     /translation="GVGRPVVDELLEPTRIYVRPVLGLLREVAVKAMAHITGGGITEN
                     LPRVLPGGRRARLTPSAWPVPPIFAAIQRAASVDDAEMRRTFNMGLGFLLVVAADDVA
                     AATQRLETAGERVFTVGEIVAGEPGVEYA"
BASE COUNT          973 a         2782 c         2629 g          909 t
ORIGIN      
        1 cgcgcgcagc aggttttctt gcaggtgccg gaggaagagg ggccggttgc cccgcttgag
       61 gctgatctcg tggtagtgga ggatcgcggc gggcacggtc tgatgcccgg gggacgggct
      121 cgccatcact cggcctccgc cggccgggcg tgcttgggcc gccgccggta gaccgtccgg
      181 ggcacctcca ccttgggcgg cttcgtcggc atcggcttgc ggaccttcat atgaatgggc
      241 atatgaatgg gcttccggcg ctgcttcatc cggcaagtat atcccgccga tccgcggtca
      301 atagggcgcg cccgggaagg tgtcgatccc ggcgggacgg cccgccgcct tgtacatctt
      361 gaccgcgccg acgcccgcgc cgccccacaa cccctcgatg accccgtcgt gcaacgccca
      421 gcttcggccg cgcacggtcc gcgccgcctc cggccggcgg aagtgcgagg agccgtcgag
      481 cgcggtgagc agggacccgc cctcggagaa gccggcgtgg atctcctcca tcacgtcgcg
      541 gcccatcagg tagccgcagc cccggcgctc gtagaggatc gccgagtggt agaagagcgg
      601 ctcgatgagg tagatctcct tgccgatcag cgtgcagaag ctctccatcg agtccagcac
      661 ccgcgacagg agccgcaggc cgcgccgcac ctgcccgggg gcgagcccgt cctgcatcgc
      721 gcgcgcttcc tccaccacgt tgcgcgacgc ggtgccgaag agggtatcgc gcccgtccgg
      781 gtcgcggtcg atcgcgaagc gcggcccggc cgggtcggtg atctgcacga aggccagctc
      841 cggcaccgac aggggcgaca tctccacgtc gaccagcagc gcgggatcgc ggtcctcggt
      901 cgaggagcgg acctccacgc gggcccacgg cttgtccgga ggcgcggtca cccgcaccag
      961 ccgcgccccg tcccggtcgc agagggtggc cggatcgatc ccgaagcggt cgagcagctc
     1021 gtcggggacc aggcgcgcgt agagggcctc cgcgacctcg cgcggcagcg cgttgatctc
     1081 ctggatcgag ccgagtccga gcccctccgc gcgcaggtcc atggggtccg gtcgcgccct
     1141 agcggggtcc cgccggcgcg gggccgggct cgagcgtggg caggagcccg cgcgcggcct
     1201 cgaggtcctc gggggtgttg acgttcatga agacgatctc gggggcgcgg tggcgggcga
     1261 cctcgtcctc cccgatcgcg aggacgcgca cgtcctcgta gaagccgacg atcttgaggc
     1321 gcccggcgcg cagccgcgtc tcgatcggcg cgaggcacgc cttgccgtag caggcgtgga
     1381 gcgtctccca ctgcgctccc acgcggggga ccaccacgtc ggcctccgcg gcccgcgcgg
     1441 tgaccaggcg cgccacctcc accatcagga acggcatgtc gcaggccacc gtgaacgcga
     1501 cgtcgccgcg cgcggcgcgg aggccggagt agatgccgcc gagcgagccg tggtccggga
     1561 acgcgtcggg caccatcggc agtccgagcg aggcgtagag ctcgggagtg ttggtgacca
     1621 ggagcacgtc gtcggcgacc gcgcgcacca cgtcggcgac ccggtcgatg atccgccgcc
     1681 cccccagctc catcagggcc ttgggccgcc cgcccatccg cacgctccgg cccccggcct
     1741 ggatcacgcc ggttatccgc atgccgcctc gacgatcacc gggccctgcc ggaggatccg
     1801 ggggggccac accgtgcagt ccgcgacggt cgagcccgcg ccccccgcgg tcggcccccc
     1861 gtcgaggatc agctccaccc gcccctcgaa gtactcgcgc accgcggccg cggtgagcgc
     1921 gggcggcgcc tcgctcggat tggcgctcgg cgcggtgatc gggcgtcccg ccgagtgcac
     1981 gagcgcgcgg gccaccgcgt ggccgggcat gcgcaccccg atggtggagc cgcccccggt
     2041 gagccccgcg ggtacgcggt ccgccgcctc cagcacgagg gtgagcgggc cgggccagta
     2101 ccgcgccatc agcgcccggg cgccgtcgga gatgcgggcg gccaggcccg ccgccatgtc
     2161 gatcgagtcg accagcacca gcagcgcctt gtgctccggc ctccccttca cctcgaacac
     2221 ccgcgcgacc gcgtcggagt cgagcgcgtc ggcgccgaga ccgtagaagc tctcggtcgg
     2281 gaacgccacc agcccgcccg cctcgaccac cgcggcggcg gccgcgatcg cgcgcacgtc
     2341 gggctgctcg ggatccaccg cgcggatacg cgtgcgaatg ggcggcgcgc tcacccgaga
     2401 cgtctcaggg cgtgatggcc gatgtcgcgc cggtagtggg ccccgtcgaa gcggatgcgc
     2461 tcgaccgcgg cgtaggcggt gcggatcgcg gcggccacgt cggcgccgag cgcctgcacg
     2521 ccgagcacgc ggccgcccgc ggtcacgagc gcgccccccg cgagcgcggt gcccgcgtgg
     2581 aacacgttga cgccggggag gccggcggcg gcctccacgc cggtgatggc ccggcccgtc
     2641 tcgtaggccc caggatatcc cgccgaggtc atgaccacgc acaccgagct ccgcgggctg
     2701 aaggccagcg cgggcggcag gcccttgccg gtcgcggtgg ccaggagcag ggccagcagg
     2761 tcgtcctcga ggcgtggcag gatcgcctgg cactcggggt cgccgaagcg gcagttgaac
     2821 tcgatgacct tcggtccgtc gcgcgtgatc atcaggccca cgaagagcac gccggtgtag
     2881 ggcgcgccct cctccgccat ggccgcgatg gtggggcgga cgatctcgcg catcacgcgc
     2941 tcctgcatgc ccgcgtccat caccggggcc ggcgagtagg cgcccatgcc gccggtgttg
     3001 gggccgcggt cgccgtcgtg caccgtcttg tggtcctgcg ccgcgacgag cggcagcacg
     3061 ccggtcccgt cggagagcgc gaagaacgac gcctcctcgc ccaccaggaa ctcctcgatc
     3121 accaccgtga gcccggagtc gccgaaggcg cgcgcctcga ggcactgcgc gaccgcccga
     3181 tccgcctcct cgaggctccc gcacaccacc acgcccttgc ccgcggcgag gccgtcggcc
     3241 ttcaccacca gcggcgcgcc cagctcccgg caatagcgcc gagccgctgc cgcgtcccgg
     3301 aaggtgccga agcgcgcggt cgggatgcgg tgacgggcca tcaggtcctt ggcgaaggcc
     3361 ttcgagctct cgagccgcgc cgcagccgcg ctgcccccaa acaccgcgag cccggccgcg
     3421 cggagccggt cggccaggcc gagggcgagc ggggtctcgg gccccaccac cacgaggtcg
     3481 ggccgctcct gctgggccag ggccacgatc tggtcgatgg cggtgtcctt gatcggcacg
     3541 cagcgggcga gcgcggcgat gccggggttc ccgggcgcgg cgaccatgcg gccgaggccc
     3601 gggctctggg cgagcttcca cgcgagcgcg tgctcgcggc cgccgccgcc gaccaggagc
     3661 acgtcggtca ttcgtcgctc tccggcgccg cgtcgtcggg gcccccggcc tcgccgtcca
     3721 ggtgggcccg cgcgatcttg gcccacgggc tgcccagatc cagctcgagg tagcgccgcc
     3781 agtggaccgc cgcgctctcg ctccgcccca tcttggccag caccccggcc aggttgaagt
     3841 gagcgtccgc gtagtcggcg tccagctcga gcgcgcgccg gtaccacgtc accgccaccg
     3901 ggaagtcgcc gaggtcctcg tggagcgagc ccaggttgaa gccggcctgg cagcagctgt
     3961 cgtcggcgtc cagcgcggcg cgatagcact cgcccgcccg ctcgtactgc cccatgcggt
     4021 ggtgcaggag gccgaggttg ttccaggccg ccgcgtagcc cggatccatg tcgagcacgc
     4081 gctcgtaggc gtcgaccgcc gactcccacc gctcggggtc gccgtcccac tccgaggcgc
     4141 gctggaacca cacctcggcc gcgtcggcgg gcgggatgag cggccgcacc atgccggtca
     4201 gcagcacctc gcgcgtctcg cgctcgaggt cgcccgccgc gaagtcgagg agcgcctgcc
     4261 cggtgcccgg gtcgaggcgc aggcggtcct gctccacgac cacgcggcgg tcgtggacca
     4321 ccagccgcag ctcggcgagc ggagtctgca cgtccgggac gaggcgccgg gcgccgtcga
     4381 gcgcggcccg cacctggcgc acgctcgcgc cgctctcgag gagctgcgcg gcggcgcggg
     4441 ccgagaccag ctcgcggaag cggaccccgc gcgggtcgtc gcgcaggagc cccaggcgcc
     4501 ggagctgcga gcgccgcttg ggggacagcc gcaggagccg cgtcagctcg cgcagcgaga
     4561 acacccgctc gggaggatga ctagtgcttg aaatggcgta tcccgcacac caccatggcc
     4621 atgtcgtgct ggtcggccgc ggccacgact tcctcgtctc ggagcgagcc ccccggatgg
     4681 atcaccgcgg tggcgcccgc cttggcgacc acgtcgagcc cgtcgcggaa cgggaagaac
     4741 gcgtcggagg cgcacaccga gcccctggtc tcgagcccga cctcgcgggc gcgcatcacc
     4801 gcgagccgcg ccgagtccac ccggttcatc tgaccggccc cgacgcccac cacctggtcc
     4861 gcggtggcga gcacgatggc gttggacttg gcgtgcttgc ccacccgcca cgcgaagcgc
     4921 agggcagaga gctccgccgc gctcggcgcc cggcgcgaga ccaccttgag gttcccctcg
     4981 tccaggtcga cgaggtcggc ctcctggagc agcaccccgc cccagacgct ccgcagctcc
     5041 ggcgcgcgcg ccggatagtc gcgccgcgcg cacgggagct ggaacacccg gcacttcttc
     5101 ttggtccggc ggatctcctc gagcgcgtcc ggctcgtagg cgggcgcgaa caggatctcg
     5161 agcaggatgc cggacagctc cttcaccacc tcgaggtcta ccgtccggtt gagccccacg
     5221 atgccgccgt agatcgagac cgggtcgcag gccttggccc ggcgaacggc ctcgcccgct
     5281 cccgccccga gggcgacgcc gcacgggttg gtgtgcttga tcaccaccgc ggccgggtcg
     5341 tcgaactcga gcagcagccc cagcgcggcc gaccagtcga gcaggttgtt gtaggagagc
     5401 tcggggccgt ggagctgggc ggccgcgctc agcccgagcg cgccggcggt cgggcggtag
     5461 aacgcggcgg cctggtgcgg gttctcgccg tagcggagcg actgcacgcg cacgccgtcg
     5521 accgccagcc gctccggaaa gcccgccgcg gcgggccgcc ccgccgcggc cgggtcgcgc
     5581 aggtaggccg cgatcgccgc gtcgtactgc gcggtgcgcc ggaaggcctc cgcggccagc
     5641 cggaagcggg tctcgtccga gagcgtcccg tcggccttcc gcagctcgtc gagcaccggc
     5701 ccgtactgcg cgggatcggt gagcaccgcc acgtgggcgt ggttcttcgc cgcgccccgg
     5761 atcatgctgg ggccgccgat gtcgatgttc tcgatcgcct cctcgagcgt gacaccggtc
     5821 cgcgccaccg tctgctcgaa cgggtagagc gtcaccgcga ccaggtcgat gggccggatc
     5881 ccgtgccgct cgagcgcggc catgtgctcg ggcagctccc gccgggccag gagcccgccg
     5941 tgcaccttgg ggtggagcgt cttcactcgc ccgtcgagca tctccgggaa gccggtgacc
     6001 tcggccacgt ccaccaccgc gatcccggac tcgcgcagca gccgggcggt gccgccggtg
     6061 gagatgatct cggccccgag cgcgaccagg ccgcgggcaa agtccaccac cccggtcttg
     6121 tcgtgcacgc tgatcagagc gcgtcctacc ttcgtcatgg cgtctccctg atggtcacgc
     6181 ggcgcccgtg gatctcgagc cgcccctccg cgaacaggcg caccgcctcc gggtagatgc
     6241 ggtgctcctc caccaggatg cgcgcggcca ggctctcctc ggtgtcgtcc ggctgcaccg
     6301 gcaccgcggc ctgcagcacc accggcccgc tgtcgacgcc ctcgtccacg aagtgcacgg
     6361 tggcgcccga cacccgcacc ccgtgggcca gggcctgccg gtgcggatgg aggcccggga
     6421 acgcggggag cagcgagggg tggatgttga ggaggcggcc cgcgaagtgc gcgacgaagc
     6481 ccgcggtcag gagccgcatg tagccggcca ggcacacgag gcccacccgg cgctcctcca
     6541 gcaccccgat caccgccttc tcgtgcgcct cccgcgaggg atgctgcctc ggatccacca
     6601 ccaccgcctc gatgcggtgc tcgcgggcga tgtcgagcac cggcgcgccc gcccggtcgg
     6661 cgacgagaac caccaccgcg gccggcatgc ggccgctctc ggcggccagg acgagggcgc
     6721 ggaagttcga gccgcggccc gaggcgagca ccccgacccg cagcggctca gacatactcg
     6781 actcccggct cgcccgcgac gatctcgcag gtcgggatag ggccgcgctc ggcagcccac
     6841 gcgagcacgc ggaagctcga gccgcggcca gaggggagca cactggctcg gaggggctca
     6901 ggcatactcg actcccggct cgcccgcgac gatctcgccc accgtgaaca cccgctcgcc
     6961 cgcggtctcg aggcgctggg tcgcagccgc cacgtcgtcg gccgcgacga ccaggaggaa
     7021 acccaggccc atgttgaacg tgcgtcgcat ctccgcgtcg tcgaccgagg ccgcgcgctg
     7081 gatggccgcg aagatcggcg gcaccggcca cgcggacggg gtgagccggg cgcgccggcc
     7141 tcccggcagc acgcggggca ggttctcggt gatgccaccg ccggtgatgt gggccatcgc
     7201 cttcacggcc acctcgcgga ggagccccag caccggacgc acgtagatgc gggtcggctc
     7261 cagcagctcg tcgaccaccg gccgacccac tcc
//
LOCUS       DATMCV010000897         2755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_845953, whole genome shotgun sequence.
ACCESSION   DATMCV010000897 DATMCV010000000
VERSION     DATMCV010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845953"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /locus_tag="VNU03_20535"
     CDS             complement(<1..1952)
                     /locus_tag="VNU03_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP76920.1"
                     /translation="MAHTFRHLFTPLRVGGLTLKNRIFSTGHAEAMAEDGKPGPQLRA
                     YHEAKARGGAGLTIVGGSTSVHPSSPASAWNMIANHDDSVIAGYRSLADAVHRHGCRV
                     MSQLTHMGRRSQSDVESWHVLLAPSQIPEKVHREVPHEIEPEQIAMLVRAFGDATRRC
                     REGGLDGVELSFAHNHLVDQFWSPLFNRRTDEYGGSLENRMRFGFEVLREIRRQVGSD
                     WVVGARISGDEMTAGGLTPADMAEIARRLAASGLVDFLSIIGGAAHTLPLQALAVPNM
                     ASPHGIFVPLAAAIKAAVAAMPIFHAGRVVDPVHADQILADGSIDLVGMTRALIADPD
                     LPRKAIEGRLDDVRLCVGANEGCIDRIYQGKPVTCVQNPGAGRERELAKPVRAASPRR
                     VVVVGAGPAGLEAARVAALRGHRVVLLEREAEVGGQVLIAARGPARAEYAGIIRFLAR
                     QVEKLTIDCRLRVEASVDTVLAESPEVVIIATGSSPYRPDLPGLDGKHVVTDRDVLLD
                     RAEVGERVVVVDDVHTQQGLSTAEYLLDRGRRVEVISRLFYAGQDVGVTSIAPLYSRL
                     FAKGAVLTPHTDLVAVEGSAVVVANVYTRTERRIEGVDTLVLSMGSRSTDALYRALKG
                     KVPTLHAVGDCVAPRGIHQAILEGTRV"
     gene            complement(2026..>2755)
                     /locus_tag="VNU03_20540"
     CDS             complement(2026..>2755)
                     /locus_tag="VNU03_20540"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP76921.1"
                     /translation="GSGIGAATAVVFAQEGAKVALADLRPADLETVAAQVKSAGGEAL
                     VMVGDVTSRPDSQRFVDETVKRFGRADLLVNSAGVAPRNAPADWDWEKVWDFVMAVNM
                     KGTFLMSRAVADQMVKQGGGSIVNLSSIYGLVGRPRELGTGLDPYVHSKGGVVQLTRD
                     MAVHFAREGVRVNALCPGFVYTNLTKALTDKPESRKFLEERHPMGRLGQPEEIAKAAL
                     FLASDDASFVTGACLPVDGGYTAQ"
BASE COUNT          419 a          994 c          948 g          394 t
ORIGIN      
        1 acccgagtcc cctcgaggat cgcctggtgg atgccgcgcg gggccacgca atcgccgacc
       61 gcgtggagcg tgggaacctt gcccttcaga gcccggtaca gggcgtcggt ggagcgactg
      121 cccatcgaca gcaccagggt gtcgacgccc tcgatccgcc gctccgtccg cgtgtacaca
      181 ttggccacca cgaccgccga gccctccacc gcgaccagat cggtgtgcgg agtcagcacc
      241 gcgcccttcg cgaacagccg cgagtacaag ggcgcgatcg aggtgacccc cacgtcctgg
      301 ccggcgtaga agagccgcga gatcacctcg acgcggcggc cgcgatcgag caagtactcg
      361 gcggtggaca gtccctgctg ggtgtggacg tcgtccacga cgacgacccg ctcgccgacc
      421 tcggcgcgat cgagaaggac atcgcggtcg gtcacgacgt gcttgccatc gagcccggga
      481 aggtcgggcc gatacggaga cgagccggtg gcgatgatca cgacctcggg tgactcggcc
      541 aggaccgtgt cgacgctggc ctcaacccga agccggcaat cgatcgtaag cttctccacc
      601 tgacgggcca ggaaacggat gatgcccgcg tactccgcgc gggccggccc gcgggcggcg
      661 atcagcacct gcccgccgac ctcggcctcg cgctcgagaa gaaccacgcg atggccacgg
      721 agggcggcca cccgggccgc ctcgaggccc gcgggcccgg cgcccaccac gacgacgcgc
      781 cggggcgaag cagcccggac cggcttggcc agctccctct cccggcccgc gccggggttc
      841 tggacgcagg tgaccggttt gccctgatag atccggtcga tgcacccctc gttggccccg
      901 acacacaggc gcacatcgtc tagccgcccc tcgatggcct tgcggggcag atcgggatcg
      961 gcgatgagcg cacgcgtcat cccgaccagg tcgatggagc cgtcggccag gatctggtcc
     1021 gcgtgaacag gatccacgac gcggccggcg tggaagatcg gcattgcggc caccgccgcc
     1081 ttgatggcgg ccgcgagggg gacgaagatg ccgtgcggcg aggccatgtt ggggacggcg
     1141 agggcttgca acggcagcgt gtgcgccgca ccgccgatga tcgagaggaa gtccacgagc
     1201 ccggaggcgg ccaggcggcg ggcgatctcc gccatgtcgg ccggggtgag gccgccggcg
     1261 gtcatctcgt cgcccgagat ccgcgcgccg accacccagt cgctcccgac ctgacgtcgg
     1321 atctcccgca ggacctcgaa gccaaaacgc atccggttct cgaggctgcc cccgtactcg
     1381 tcggtccgcc ggttgaagag cggtgaccag aactggtcca ccagatggtt gtgagcgaag
     1441 gacagctcca ccccgtccag cccgccctcg cggcagcgcc gcgtcgcatc cccgaaggcg
     1501 cgcaccagca tggcgatctg ctcgggctcg atctcgtgcg gcacctcgcg atggaccttc
     1561 tccggaatct gcgacggcgc caggaggacg tgccagctct ccacgtcgga ctgcgaccgc
     1621 cggcccatgt gggtgagctg gctcatgacc cggcagccgt ggcggtgcac cgcgtccgcg
     1681 agactccgat agccggcgat gaccgagtcg tcgtggttgg cgatcatgtt ccaggcgctg
     1741 gccggcgagg aggggtggac gctggtcgag ccgccgacga tggtgaggcc cgcgccgccg
     1801 cgggccttgg cttcgtggta ggcgcggagc tgggggccgg gcttgccgtc ctcggccatc
     1861 gcctcggcgt gcccggtcga gaagattcgg ttcttgaggg tgagcccgcc gacccggaga
     1921 ggtgtgaaga gatggcgaaa cgtgtgcgcc atggggcaca gcatggacgg gagcggacgg
     1981 ctcgtcaacg caggaggagt ggccccgact cgtttcgcgc ggcacctact gtgcggtgta
     2041 gcccccgtcc accggcaggc atgcccccgt gacgaaggag gcgtcgtccg acgccaggaa
     2101 gagcgccgcc tttgcgatct cctcgggctg gccgagccgg cccatcgggt gcctctcttc
     2161 caggaacttc cggctctcgg gcttgtcggt cagggccttc gtgaggttgg tgtacacgaa
     2221 cccggggcac agggcgttca cccgcacgcc ctcccgggcg aagtgcaccg ccatgtcccg
     2281 ggtgagctgg accaccccgc ccttgctgtg cacgtacggg tcgagcccgg tcccgagctc
     2341 tcgtggtcgg cccacgaggc cgtagatcga ggacaggttc acgatcgacc cgcccccctg
     2401 cttcaccatc tggtccgcga ccgcccggga catgagaaac gtgcccttca tgttgacggc
     2461 catcacgaag tcccagacct tctcccagtc ccagtcggcc ggcgcgttcc ggggggccac
     2521 cccggcgctg ttcacgagca ggtccgcccg gccgaatcgc ttcaccgtct cgtcgacgaa
     2581 ccgctgcgag tcgggccggc tcgtcacgtc gcccaccatc accagcgcct cgccgccggc
     2641 gctcttgacc tgggccgcga cggtctccag atccgccggc cgcaggtcgg cgagcgccac
     2701 cttcgcgccc tcttgcgcga acaccaccgc cgtcgccgcg ccgatgcctg atccc
//
LOCUS       DATMCV010000898         2055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_781887, whole genome shotgun sequence.
ACCESSION   DATMCV010000898 DATMCV010000000
VERSION     DATMCV010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781887"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VNU03_20545"
     CDS             <1..817
                     /locus_tag="VNU03_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HWP76922.1"
                     /translation="KSGLIGRKIAATLASTGTPAYFLHPAEGVHGDLGMVAREDVVVA
                     LSNFGETDEVLALLPALKRLGIPLVLLTGAPASTLARQADIVIDVGVAEEACPMNLAP
                     TSSTTAALAMGDALAMALLDLRGLRPEDYAALHPRGSLGWKSLFKVGDLMHTGEAIPV
                     VSEQATMKEAIEEMTGKGFGITTVVDDGGRLVGILTDGDLRRQQLAHGTSLLERRAGE
                     CMTRDPKVIGAEELATRALALMEGRITSLVITDAAGRPAGVIRLHDILLAKIV"
     gene            814..1317
                     /locus_tag="VNU03_20550"
     CDS             814..1317
                     /locus_tag="VNU03_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWP76923.1"
                     /translation="MTAAPRASRIRLLVLDVDGVLTDGGLLYGASGEETKRFHVHDGL
                     ALVAARKAGLQIAVLSSRASAAVTRRMTELGVSEVHQGVGDKGSALTALRERLGIEAA
                     EVAVMGDDLPDLAAMAHAGLALAPANAVAEVKRAADWVARRRGGDGAVREAVEMLLKA
                     RRAWPPR"
     gene            1386..1994
                     /gene="lptC"
                     /locus_tag="VNU03_20555"
     CDS             1386..1994
                     /gene="lptC"
                     /locus_tag="VNU03_20555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HWP76924.1"
                     /translation="MRKAPFIILTLVVVFLSVVVGVLVHRARVPRQLTTEPAPTNADY
                     RLKQVHLQEQGRDGSRWQLDAEYSETFEEQNKTTMKKVTVKVDQPSKGDQASRSWTVT
                     GDEGDLNQETKNVELRGNVVLISSDGLRLETDRLRWDAEAQRAWTEDPVVIYRAGAVV
                     HGTGFESKVAEEISSIKGRVRATFKKGTGVGVPALAGQGGGS"
BASE COUNT          308 a          703 c          756 g          288 t
ORIGIN      
        1 caagtcgggc ctcatcggac gcaagatcgc ggcgaccctg gcgagcaccg ggacccccgc
       61 ctacttcctc catcccgcgg aaggagtcca cggtgacctg gggatggtgg cgcgcgagga
      121 cgtcgtcgtc gcgctgtcca acttcggcga gaccgacgag gtactggcgc tgctgcccgc
      181 gctcaagcgc ctcggcatcc cgctggtcct cctcaccggg gcccccgcct cgacgctcgc
      241 gcgccaggcc gacatcgtga tcgacgtggg agtcgcggag gaggcctgcc ccatgaatct
      301 cgcgccgacc tcgagcacca ccgcggcact ggcgatgggc gacgcgctcg ccatggcgct
      361 cctcgatctg cgtggcctgc gtccggagga ctacgcggcg ctgcacccgc gcgggagcct
      421 cgggtggaag tcgctcttca aggtcggcga cctgatgcac accggggagg cgatcccggt
      481 ggtctcggag caggccacga tgaaggaagc catcgaagag atgaccggca agggattcgg
      541 gatcaccacc gtcgtggacg acgggggccg cctggtcggg atcctcaccg acggtgacct
      601 gcgccgccag cagctcgccc acgggaccag cctgctcgag cggcgcgcgg gggaatgcat
      661 gacccgcgac ccgaaggtga tcggggccga ggagctcgcc acgcgggcgc tggccctgat
      721 ggaagggcgg atcacgagcc tggtgatcac cgacgcggcg ggccgcccgg ccggggtgat
      781 ccggctgcac gacatcctgc tcgccaagat cgtgtgaccg ccgctccccg cgcttcccgc
      841 attcgcctcc tcgtcctcga cgtcgacggg gtgctgaccg acggcggcct cctctacggc
      901 gcctccgggg aagagacgaa gcgcttccac gtccacgacg gcctggccct ggtggccgcg
      961 cgcaaggcgg ggctgcagat cgcggtgctc tcctcgcggg cctccgccgc ggtcacccgg
     1021 cgcatgaccg agctcggcgt ctccgaggtg caccagggcg tgggcgacaa gggcagcgcc
     1081 ctgaccgcgc tccgcgagcg gctcgggatc gaggccgccg aggtcgcggt gatgggcgat
     1141 gacctccccg atctggccgc gatggcccat gccgggctcg cgctcgcgcc cgccaatgcg
     1201 gttgccgagg tcaagcgcgc ggcggactgg gtggcgcgcc gccggggcgg cgacggggcg
     1261 gtgcgagagg cggtggagat gctcctaaag gcccgccggg cttggccccc gcgctgatcg
     1321 ctcgccgact gcttcttcga ttgcctggaa tcgagtacct gtgctagcgt ggcacggaga
     1381 ttgctatgcg caaggcaccg ttcattatcc tgacgctcgt cgtggtattc ctatcggtgg
     1441 tggtgggtgt gctcgttcac cgggcccggg tgccccggca gctgaccacc gagcccgccc
     1501 cgaccaacgc ggactaccgg ttgaagcagg tccacttgca agagcagggc cgagacggct
     1561 cgcggtggca gctcgacgcg gagtactccg agacgttcga ggagcagaac aagacgacga
     1621 tgaagaaggt gaccgtcaag gtggaccagc cgtccaaggg cgatcaggcg tcgcgctcgt
     1681 ggaccgtcac cggcgacgag ggcgacctga atcaggagac caagaacgtg gagctgcgcg
     1741 gcaacgtggt cctgatctcg agcgacggcc tccggctcga gaccgaccgt ctccgctggg
     1801 acgcggaggc gcagcgcgcc tggaccgagg atccggtggt gatctatcgg gcgggcgcgg
     1861 tggtccacgg caccggcttc gagtcgaagg tggccgagga gatcagcagc atcaaggggc
     1921 gggtccgcgc gacgttcaag aagggcaccg gcgtcggcgt ccccgccctc gcgggacagg
     1981 gcggaggctc gtgatccgac ggatcgcggt cggcgcgctc gcggtctggg cggccctcgc
     2041 tttcgccggc cccgg
//
LOCUS       DATMCV010000899         8448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_115461, whole genome shotgun sequence.
ACCESSION   DATMCV010000899 DATMCV010000000
VERSION     DATMCV010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115461"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VNU03_20560"
     CDS             <1..704
                     /locus_tag="VNU03_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76925.1"
                     /translation="GFAGKLYAAHLSGLARYRQVLARRNHLLQSGMVGAALRSALTPW
                     SEQLARIGLELVARRLSALEALQEEARALYPTLAGSGDVTLRYSGGLPADATEADVLA
                     AFDEHFVEETRRGQTVVGPHRDDLLIAVDERDLRLYGSRGQQRLMALALRLAEAGPVA
                     RAIGSAPVLLLDDALSELDPQVQARVLEHVAGAGQVFLTTADGALPETRRVAWWEVKE
                     GRVTDPTLDRVRGAA"
     gene            704..3079
                     /gene="gyrB"
                     /locus_tag="VNU03_20565"
     CDS             704..3079
                     /gene="gyrB"
                     /locus_tag="VNU03_20565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HWP76926.1"
                     /translation="MGDTYTASDIKVLEGLEAVRKRPAMYIGDTGTYGLHHLVYEAVD
                     NSVDEALAGYCDSIKVILHSDGSCSVGDNGRGIPTEIHKESGRPAAEVVLTVLHAGGK
                     FEHSAYKVSGGLHGVGISVVNALSEWLELEIRRQGKVWTQRYEYGAPQGEITAGEKTT
                     KHGTIVRFKPDTKIFEETTFSFDTLSNRLRELAFLNKGLKIVIEDERDGRSHTFLYRG
                     GIIEFIKHLNQNKTPIHPKVLFFEGKKDNIEVEVALQYNDGYQESVFSFANNINTREG
                     GTHLTGFRAALTGTISNYAKANGFLKSFKGDVSGDDVREGLTAVVSVRIPDPQFEGQT
                     KAKLGNSDVKGLVQQIVNDRLGEAFEEDPTTARKIADKCVRAAQAREAARKARELTRK
                     GGRDDEGLSAKLADCSERDPQLREIFLVEGDSAGGSAKQGRDRKVQAVLPLRGKIINA
                     EKARYDKVLSHNEIRLLISALGTGIGPEEFDVSKLRYHKVILMTDADVDGAHIRTLLL
                     TFFFRHMVQVIEAGHLFIAQPPLFKVKKGRAERYLMSEREMEEFLLEQWVEKASIKVP
                     GKAAPLRADALLEALKHVLEFRALFSKIVRRGIPRAILDGLLRKKFRASKRGVGDAEI
                     AAAVAEAAGELPGWSAQLTGGDNGDAGAIQVAGPHVAQFSVDVLKSPDYAQLLELHAD
                     IAGLHKGPCTIVDAAEKETPTKSLNQLMGAIMEQAKDGATLQRYKGLGEMNPEQLWET
                     TMNPETRTLLRVTMEDAVGADEMFTVLMGDAVEPRREFIEKFALDVVNLDI"
     gene            3098..5542
                     /gene="gyrA"
                     /locus_tag="VNU03_20570"
     CDS             3098..5542
                     /gene="gyrA"
                     /locus_tag="VNU03_20570"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWP76927.1"
                     /translation="MSPERQTPVPIEEEMRKSYLDYAMSVIVGRALPDIRDGLKPVHR
                     RVLFAMHELGLNWNRAYKKSARVVGEVLGKYHPHGDAPVYEALVRMVQEFSLRYPLVD
                     GQGNFGSIDGDPPAAMRYTETRLAKIAHELLADIDKETVDFTPNFDESLQEPVVLPTK
                     VPNLLVNGSSGIAVGMATNIPPHNLREVVDGLVRVIDDPEVSIDELIKLIPGPDFPTR
                     GYIYGRGGIREAYTTGRGIITLRAKAHVEKMRGGREAIIVTELPYQVNKASLMEKIGE
                     LVRDKRIEGISERRDESSREGIRVVLELGRGEMAQIVINQLYKHTQMQTTFGVIMLAL
                     VGRRPQVVNLKQMLQEFIAFRREVVTRRTQYDLARAEERAHILEGLRKAVDQLDLVIR
                     LIRLAESPDAAKDALMRQLDLSEIQAKAILDMRLQRLTQLERHKIVEEHEQTLALIAD
                     LKGILASEARLLGIIKDELAALREEFGDERRTEILAETTDLTIEDLLADEDMVVTITR
                     SGYIKRTHVEAYRSQKRGGKGVTGMETKEEDIVEDLFVASTHSFLLFFTNKGKVHWLK
                     VHEIPEGGRQAKGKAMANVLSLAENERVATCVPVRDFESGGYVLFATKQGKVKKTELS
                     AFSHPRAGGIQAITLEDGDEVMGARRTDGQREVLLSTKQGMIIRFPEDEVRPMGRTAA
                     GVRGIDVDEGDQVIAAETLKEGVTILTVTERGYGKRTPLDEYRLQGRAGKGIIDIKTA
                     GRNGTVVGMLQVREGDDILVVTTKGKIIRVHADEVTSQGRNTMGVRIIDLDADDQVGN
                     LARVEAEQAAPEVPSE"
     gene            5606..6907
                     /gene="serS"
                     /locus_tag="VNU03_20575"
     CDS             5606..6907
                     /gene="serS"
                     /locus_tag="VNU03_20575"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWP76928.1"
                     /translation="MLDIKLIREDPTTVERALATRGAEIALAPVLAADTERRRLVTEA
                     EDLKAERNRASEAIGQAKRRGEDPQGATARMRELSDRIKALDGLVKEADARLEGLLLE
                     VPNLPHASVPVGRSADDNVEVRRWGAPRQFAFEPKQHFEIGEALGILDFDRASKIAKS
                     RFTVMWGAASRLNRALVQLMLDLHTREHGFTEVWVPHLVNPETMVGVAMLPKFEEQMF
                     RTVEPDAGRTLYLIPTAEVALTALHGAEILAEAELPKRYCAFTPCYRREAGTYGQDTK
                     GMIRQHQFDKVEMVKVVAPGHSYDELESMVESAEAVLQRLELPYRVVALCTGDMGFHS
                     AKTYDLEVWLPGQGKYREISSCSNCEGYQARRLDLKYRPAGGGRVELCHTLNGSGLAV
                     GRTLIAVLENYQEADGSVTVPAALRPYMGGLERIGPTGAGR"
     gene            6916..7005
                     /locus_tag="VNU03_20580"
     tRNA            6916..7005
                     /locus_tag="VNU03_20580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6952..6954,aa:Ser,seq:tga)
     gene            complement(7023..>8448)
                     /locus_tag="VNU03_20585"
     CDS             complement(7023..>8448)
                     /locus_tag="VNU03_20585"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HWP76929.1"
                     /translation="TERYLGAWLADTAYYRMQLTAGETDNPDQRISEDLNLFVAGTLT
                     LGIGGMRAVVTLVSFLGILWGLSGAFALPFWPYWTVPGYMVWAALVYAIIGTFFTNLI
                     GRPLVRLNFNQQRYEADFRFGLVRFRENTEGVALYHGEGDEMRSFRERFAAVVRNYWG
                     IMRQQKRLTWFTSAYGQAAIIFPFVVAAPRYFRGEIPLGGLVQTATAFGQVQDSLSFI
                     VTAYPDIAQWRSVVARLVGFEQTLARVQADKKADGVRHRPDMAGGLALENVDLSLPGG
                     RPLIEGVSLGLRPGDTALISGPSGVGKSTLFRAIAGIWPFGRGEIRLPSEGRVLFLPQ
                     KPYLPIGTLREVVSYPMPSVGIDDATLREALELVGLPSLVSRLDETAHWALALSPGEQ
                     QRIAFARALVQKPAWLFLDEATSAVDEDTEARLYALVRDRLPGTTVVSVGHRRSLESF
                     HSRRLVVTPNGTGPATVTEAPARA"
BASE COUNT         1485 a         2839 c         2980 g         1144 t
ORIGIN      
        1 acggcttcgc gggcaagctc tacgcggccc acctgagtgg tctcgctcgc taccgccagg
       61 tcctggcgcg gcgcaatcat ctgctccaga gcgggatggt gggggccgcg ctccgcagcg
      121 cactcacccc gtggagcgag cagctggcgc ggatcggcct cgagctggtg gcccgacgcc
      181 tgagcgcgct ggaggccctc caggaggagg cccgcgcgct ctacccgaca ctggctggat
      241 cgggggacgt gacgctgcga tacagtgggg gcctcccggc ggacgcgacc gaggccgacg
      301 tcctggccgc gttcgacgag cacttcgtcg aggagacgcg gagaggccag acggtggtcg
      361 ggccgcatcg cgacgatctc ctgatcgcgg tggacgagcg cgatctgcgt ctctacggct
      421 cgcgcgggca gcagcggctg atggcgctcg cgctgcgcct ggccgaggcc gggccggtgg
      481 cgcgcgcgat cggcagcgcg ccggtgctgc tgctcgacga cgcgctctcc gagctggatc
      541 cccaggtgca ggcccgcgtg ctggagcacg tggccggcgc gggccaggtc tttctgacca
      601 ccgccgacgg tgcgctgccg gagacgaggc gggtggcctg gtgggaggtg aaagagggtc
      661 gcgtgacgga cccgacgctc gaccgcgtgc gaggagccgc ctgatgggcg acacctacac
      721 cgcgagcgac atcaaggtcc tcgaggggct ggaggcggtc cgcaagcgcc cggccatgta
      781 catcggcgac accggcacct acggcctcca ccacctggtc tacgaggcgg tcgacaactc
      841 ggtcgacgag gcgctggccg gctactgcga cagcatcaag gtgatcctgc actcggacgg
      901 ctcctgctcg gtgggggaca acggacgcgg gatcccgacc gagatccaca aggagagcgg
      961 ccgccccgcc gccgaggtgg tgctcaccgt gctccacgcg ggcggcaagt tcgagcactc
     1021 cgcttacaag gtctcgggcg ggctgcacgg cgtcggcatc tcggtggtca acgcgctctc
     1081 ggagtggctt gagctcgaga tccgccgcca gggcaaggtg tggacccagc gctacgagta
     1141 cggggcgccg cagggggaga tcaccgcggg cgagaagacg accaagcacg gcaccatcgt
     1201 gcgcttcaag cccgacacca agatcttcga ggagaccacg ttctccttcg acacgctctc
     1261 gaaccggctg cgcgagctcg cgttcctgaa caagggcctc aagatcgtca tcgaggacga
     1321 gcgggacggg cgcagccaca ccttcctcta caggggaggg atcatcgagt tcatcaagca
     1381 cctgaatcag aacaagaccc cgatccaccc gaaggtgctc ttcttcgagg gcaagaagga
     1441 caacatcgag gtcgaggtgg cgctccagta caacgacggg taccaggaga gcgtgttctc
     1501 cttcgccaac aacatcaaca cccgcgaggg cggcacgcac ctgaccggct tccgggccgc
     1561 gctcaccggc accatctcga actacgccaa ggccaacggc ttcctcaaga gcttcaaggg
     1621 ggacgtcagc ggcgacgacg tgcgcgaggg cctgaccgcg gtggtctcgg tgcggattcc
     1681 ggatccgcag ttcgagggcc agaccaaggc caagctcggc aactccgacg tcaaggggct
     1741 cgtgcagcag atcgtcaacg accgcctcgg ggaggcgttc gaggaggacc cgaccaccgc
     1801 gcggaagatc gccgacaagt gcgtgcgggc ggcccaggcc cgcgaggccg cgcggaaggc
     1861 gcgcgagctc acccgcaagg gcgggcgcga cgacgagggt ctctccgcca agctcgccga
     1921 ttgctcggag cgcgatccgc agctccgcga gatcttcctg gtcgagggcg actctgcggg
     1981 cggctccgcc aagcaggggc gcgaccgcaa ggtgcaggcg gtgctgccgc tgcgcggcaa
     2041 gatcatcaac gcggagaagg cgcgctacga caaggtcctg tcccacaatg aaatccggct
     2101 gctgatctcg gcgctcggca ccgggatcgg ccccgaggaa ttcgacgtca gcaagctgcg
     2161 ctaccacaag gtcatcctga tgacggacgc cgacgtggac ggcgcccaca tccggaccct
     2221 gcttctgacc ttcttcttcc ggcacatggt ccaggtgatc gaggccggcc acctgttcat
     2281 cgcgcagccg ccgctcttca aggtgaagaa ggggcgggcc gagcggtacc tgatgagcga
     2341 gcgggagatg gaggagttcc tgctcgagca gtgggtggag aaggcgagca tcaaggtgcc
     2401 cggcaaggcg gccccgctcc gggccgacgc gctgctggag gcgctcaagc acgtgctgga
     2461 gttccgcgcc ctcttctcca agatcgtccg ccgcggcatc ccgagagcga tcctggacgg
     2521 actgctccgc aagaagttcc gcgccagcaa gcgcggggtg ggcgacgccg agatcgcggc
     2581 cgcggtggcc gaggcggcgg gcgagctccc cgggtggtcc gcgcagctca ccggcggcga
     2641 caacggcgac gcgggggcca tccaggtcgc ggggccgcac gtggcgcagt tcagcgtcga
     2701 cgtactcaag tcgcccgact acgcccagct cctcgagctg cacgcggaca tcgcgggcct
     2761 gcacaagggc ccgtgtacga tcgtggacgc ggccgagaag gagaccccga ccaagagcct
     2821 aaaccagctg atgggcgcga tcatggagca ggccaaggac ggggccacgc tccagcgcta
     2881 caagggcctc ggcgagatga accccgagca gctgtgggaa acgaccatga acccggagac
     2941 gcgcaccctc ctccgtgtga ccatggaaga cgcggtcggc gccgacgaga tgttcacggt
     3001 cctgatgggc gacgcggtgg agccgcgccg cgagttcatc gagaagttcg ccctcgacgt
     3061 cgtcaacctc gacatctagg agatccggac ccgttccatg agcccagagc gccagacccc
     3121 ggttccgatc gaagaggaga tgcggaagtc gtatctcgac tacgccatgt ccgtcatcgt
     3181 cggccgggcg ctgcccgaca tccgcgacgg cctcaagccg gtgcaccggc gggtgctctt
     3241 cgcgatgcac gagctcgggc tcaactggaa tcgcgcgtac aagaagtcgg cgcgggtggt
     3301 cggcgaggtg ctcggcaagt accatccgca cggcgacgcg ccggtgtacg aggcgctcgt
     3361 ccgcatggtg caggagttct ccctgcgcta cccgctggtg gacgggcagg gcaacttcgg
     3421 ctcgatcgac ggcgatccgc ccgccgcgat gcggtacacc gagacgcgcc tcgccaagat
     3481 cgcccacgag ctgctggccg acatcgacaa ggagacggtg gatttcacgc cgaacttcga
     3541 cgagtcgctc caggagccgg tggtgctgcc gaccaaggtg ccgaacctgc tggtcaacgg
     3601 ctcctccggg atcgcggtcg ggatggccac caacatcccg ccccacaacc tgcgggaggt
     3661 ggtggacggg ctcgtgcggg tgatcgacga tcccgaggtc agcatcgacg agctgatcaa
     3721 gctgatcccg ggaccggact ttcccacccg cggctacatc tacggccgcg ggggcatccg
     3781 ggaggcctac accaccgggc gcggcatcat caccctgcgg gccaaggccc acgtggagaa
     3841 gatgcggggc gggcgcgagg cgatcatcgt caccgagctg ccctaccagg tgaacaaggc
     3901 ctcgctgatg gagaagatcg gcgaactcgt gcgcgacaag cggatcgagg gcatcagcga
     3961 gcgccgcgac gagtcgagcc gcgagggcat ccgcgtcgtg ctggagctgg gccgcggcga
     4021 gatggcccag atcgtgatca accagctcta caagcacacc cagatgcaga ccaccttcgg
     4081 ggtgatcatg ctggccctcg tgggccgccg cccgcaggtc gtcaacctca agcagatgct
     4141 ccaggagttc atcgcgtttc gccgggaggt cgtcacccgc cggacccagt acgacctcgc
     4201 gcgcgcggaa gagcgggccc acatcctgga gggcctgcgc aaggcggtcg accagctcga
     4261 cctcgtcatc cgcctcatcc gcctggcgga gagcccggac gcggccaagg acgccctgat
     4321 gcgccagctg gatctctcgg agatccaggc caaggccatc ctcgacatgc ggctgcagcg
     4381 cctgacccag ctcgagcgcc acaagatcgt cgaggagcac gagcagacgc tggccctcat
     4441 cgcggacctc aagggcatcc tggcgtccga ggcgcgcctg ctcgggatca tcaaggacga
     4501 gctcgccgcg ctccgcgagg agttcggcga cgagcgccgc accgagatcc tggccgagac
     4561 caccgacctc accatcgagg acctgctcgc ggacgaggac atggtggtca ccatcacgcg
     4621 ctccggctac atcaagcgga ctcacgtgga ggcctaccgc agccagaagc gcggcggcaa
     4681 gggcgtcacc gggatggaga ccaaggaaga ggacatcgtc gaggacctgt tcgtggcctc
     4741 gacccactcc ttcctcctgt tcttcaccaa caagggcaag gtgcactggc tcaaggtgca
     4801 cgagatcccg gagggcggcc gccaggcgaa gggcaaggcg atggccaacg tgctgtccct
     4861 cgccgagaac gagcgggtgg ccacctgcgt cccggtgcgc gacttcgagt cgggcggcta
     4921 cgtgctcttc gccaccaagc agggcaaggt gaagaagacc gagctgtccg ccttctctca
     4981 cccgcgcgcg ggcggcatcc aggccatcac cctcgaggac ggcgacgagg tcatgggggc
     5041 ccggcgcacc gacggccagc gcgaggtcct gctctccacc aagcagggca tgatcatccg
     5101 gttcccggag gacgaggtcc gcccgatggg ccgcaccgcc gccggcgtcc gcggcatcga
     5161 cgtggacgag ggggatcagg tcatcgcggc ggagaccctc aaggaaggcg tgacgatcct
     5221 gaccgtgacc gagcgcggct acggcaagcg caccccgctc gacgagtacc ggctgcaggg
     5281 gcgggcgggc aagggcatca tcgacatcaa gaccgccggg cgcaacggga cggtggtcgg
     5341 gatgctccag gtccgcgagg gggacgacat cctggtggtc accaccaagg gcaagatcat
     5401 ccgcgtgcac gccgacgagg tgacgagcca gggacggaac accatgggcg tccgcatcat
     5461 cgatctcgac gccgacgacc aggtgggcaa cctcgcccgg gtggaagccg agcaggccgc
     5521 ccccgaggtg ccgtcagagt agacctcgcc gcggcgggag cccggcgagg ggacggaggc
     5581 cgcgcagctg cggcctcttt tggcgatgct cgacatcaag ctgatccgcg aagacccgac
     5641 gacggtggag cgcgccctcg cgacgcgcgg cgccgagatc gcgctcgccc cggtgctggc
     5701 ggcggacacc gagcgtcgcc ggctggtgac cgaggccgag gacctcaagg ccgagcgcaa
     5761 ccgcgcctcc gaggccatcg ggcaggccaa gcggcgcggc gaggatccgc agggtgcgac
     5821 ggcgcggatg cgtgaactct cggatcgcat caaggccctc gatgggctcg tgaaggaggc
     5881 cgacgcgcgg ctcgaaggcc tgctgctcga ggtgccgaac ctgccgcacg cgagcgtgcc
     5941 ggtgggccgc tcggccgacg acaacgtcga ggtgcgccgc tggggcgcac cacggcagtt
     6001 cgctttcgag ccgaagcagc acttcgagat cggcgaggcc ctcggcatcc tcgacttcga
     6061 ccgggcgagc aagatcgcca agtcgcgctt caccgtgatg tggggcgcgg cgtcgcggct
     6121 caaccgggcg ctcgtgcagc tcatgctgga tctccacact cgcgagcacg gattcaccga
     6181 ggtctgggtg ccgcacctgg tgaacccgga gaccatggtc ggcgtggcga tgctgcccaa
     6241 gttcgaggag cagatgttcc ggacggtgga gcccgacgcg ggccgcaccc tctacctgat
     6301 cccgaccgcg gaggtcgcgc tcaccgcgct gcacggggcc gagatcctgg ccgaggccga
     6361 gctgccgaag cggtactgcg cgttcacccc ctgctatcgc cgcgaggcgg gcacctacgg
     6421 ccaggacacc aagggcatga tccgccagca ccagttcgac aaggtcgaga tggtgaaggt
     6481 cgtcgcaccc ggccactcct acgacgagct cgagtccatg gtggagagcg cggaggcggt
     6541 cctgcagcgg ctcgagctgc cgtaccgggt ggtggcgctc tgcaccggcg acatggggtt
     6601 tcactccgcc aagacctacg acctcgaggt gtggctaccg gggcagggca agtaccgtga
     6661 gatctcctcg tgctcgaact gcgagggcta ccaggcgcgc cggctcgacc tcaagtaccg
     6721 cccggcgggc ggcgggcgtg tggagctctg ccacaccctc aacggatcgg ggctcgcggt
     6781 cgggcgcacg ctgatcgcgg tgctcgagaa ctaccaggaa gccgacgggt ccgtcacggt
     6841 gccggccgcg ctccgcccgt acatgggcgg cctcgagcgc atcgggccga cgggcgcggg
     6901 acgctagtca gacgcggagg ggtggccgag tccggttgaa ggcgccggtc ttgaaaaccg
     6961 gaatcccgca agggatcggg ggttcgaatc cctccccctc cgccattttt ttgctgctgc
     7021 gctcaggctc gcgccggcgc ctccgtgacc gtcgcaggcc ccgtgccgtt gggcgtgact
     7081 acgaggcggc gcgagtggaa gctctcgagg ctgcgccggt ggcccacgct gaccaccgtg
     7141 gtgccgggca ggcggtcgcg gacgagcgcg tagagccggg cctcggtgtc ctcgtccacc
     7201 gccgaggtag cctcgtcgag gaacagccac gcgggcttct gcacgagcgc gcgggcgaag
     7261 gcgatgcgct gctgctcccc cggcgacagc gcgagcgccc agtgcgcggt ctcgtccaag
     7321 cgggacacca ggctgggtag accgaccagc tccagggctt cacgcagcgt cgcgtcgtcg
     7381 atcccgacgg acggcatcgg gtagctcacc acctcccgga gcgtaccgat cggcagatag
     7441 ggcttctggg gcaggaagag cacccggccc tcgctcggca gtcgaatctc cccgcgcccg
     7501 aagggccaga tgccagcgat ggcacggaag agcgtgctct tgcctactcc cgacgggccg
     7561 ctgatgagcg cggtgtcgcc gggccggaga ccgagcgaga ccccctcaat gagcgggcga
     7621 ccgcccggca ggctcagatc gacgttctcc aacgcgagcc ccccggccat gtccgggcgg
     7681 tggcgaactc cgtccgcctt cttgtcggcc tggacccgag ccaaggtttg ctcgaagccg
     7741 accaaacggg ccaccacgga gcgccattgg gcaatgtccg ggtaggcggt cacgatgaag
     7801 gagagcgaat cctggacttg gccgaaggcg gtcgcggtct gaacgaggcc tccgaggggg
     7861 atctcaccac ggaagtatcg cggcgcggct accacgaacg ggaagatgat cgcggcctgg
     7921 ccataggcgg acgtgaacca ggtgagccgc ttctgctggc gcatgatgcc ccagtagttg
     7981 cgcaccaccg cggcgaaccg ttcgcgaaag ctgcgcatct cgtcgccctc gccgtggtag
     8041 agggcgacac cctccgtgtt ctcccggaag cggacgaggc cgaatcggaa gtcggcctcg
     8101 taccgctgct ggttgaagtt gaggcgcacg agcgggcggc cgatcaggtt ggtaaagaac
     8161 gtcccgatga tcgcgtagac gagggcggcc cacaccatgt acccgggcac cgtccagtag
     8221 ggccagaacg gcaaggcgaa tgccccggag agcccccaca ggatgccgag gaacgatacc
     8281 agggtaacca ccgctcgcat cccgccgatg ccaagggtca gggtgcctgc cacgaacagg
     8341 ttcagatcct cggagatgcg ctggtcgggg ttgtccgtct cgccggcggt gagctgcatg
     8401 cggtagtagg cggtatcggc gagccaggct ccgaggtacc gctcggtg
//
LOCUS       DATMCV010000900         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_315800, whole genome shotgun sequence.
ACCESSION   DATMCV010000900 DATMCV010000000
VERSION     DATMCV010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315800"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VNU03_20590"
     CDS             complement(<1..1391)
                     /locus_tag="VNU03_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HWP76930.1"
                     /translation="MPSAADFEKLGVFYLGRPYDPQQKKAQDGVLLYDSKDLVTHAVC
                     VGMTGSGKTGLCLALIEEAAIDGIPAILIDPKGDLANLLLTFPALRPEDFRPWINEDD
                     ARRKNLSADDYARQQAEQWQKGLAAWGEDGARIQRLRDAADFAIYTPGSNAGIPLSIL
                     KSFAAPPSAIREDEELLRERIGTTATSLLGLLGIDADPIKSREHILIATILDAAWRQG
                     RELDLAGLIQQIQTPPVKRVGVLDLDAFFPSKDRFALAMSLNNLLAAPGFGAWMEGEP
                     LDVDALLHTPEGKPRVSIVSIAHLSDAERMFFVSLLLNQVVGWMRTQSGTTSLRALLY
                     MDEIFGFFPPVANPPSKTPLLTLLKQARAFGVGVVLATQNPVDLDYKGLSNTGTWLIG
                     RLQTERDKARVLDGLEGAAGTAGQGFDRAGLERILSGLSTRVFLMNNVHEDHPVVFES
                     RWVMSYLRGPLTRA"
     gene            complement(1412..>2317)
                     /locus_tag="VNU03_20595"
     CDS             complement(1412..>2317)
                     /locus_tag="VNU03_20595"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76931.1"
                     /translation="GGADYGFREAAETGLYPGPRMLVSGRPLSQTGGHADKRRRAEWI
                     EPVGCCVGMAGMIADGADEVRKAARENLRHDVDQIKIMASGGAMSPGDELDTTQYTVD
                     EMRAAVEEARAVGKYVLAHAYSGSAIRNAIQAGVRCIEHGNLLDEAGAQAIKAAGAYL
                     VPTMVTYEAISREGKNYGIGDHQIQKINMALEKSLEGLTHAYRAGCKIGSGSDLLGDM
                     QAQRAMEFELKGQVMKPMEVLLSATMVNAEIFRMTDKIGSVEPGKYADLLVVDGNPLK
                     NLRVFQNQANLHAIVKGGVFVKRAI"
BASE COUNT          320 a          794 c          823 g          380 t
ORIGIN      
        1 gcgcgcgtga gcggcccgcg caggtaggac atcacccagc ggctctcgaa cacgaccggg
       61 tgatcctcgt gcacgttgtt catcaggaag acgcgggtcg agagcccgga aaggatccgc
      121 tcgagcccgg cgcggtcgaa gccctggccc gcggtgcccg ccgccccctc gaggccgtcg
      181 agcacgcgcg ccttatcgcg ctcggtctgg agccgcccga tgagccaggt gccggtgttg
      241 gacaggccct tgtagtcgag gtcgaccggg ttctgggtgg cgagcaccac gcccaccccg
      301 aaggcgcgcg cttgcttgag cagcgtgagg agtggcgtct tcgacggcgg gttggccacc
      361 gggggaaaga agccgaagat ctcgtccatg tagagcagcg cgcgcaggct cgtggtgccg
      421 gactgggtgc gcatccagcc caccacctga ttgagcagca gcgagacgaa gaacatccgc
      481 tccgcgtcgc tcaggtgcgc gatcgacacg atggagacgc gcggcttgcc ctccggcgtg
      541 tgcaggaggg cgtcgacgtc gaggggctcg ccctccatcc aggccccgaa gccgggggcc
      601 gccagcaggt tgttgagcga catcgcgagg gcgaagcgat ccttggaggg gaagaacgcg
      661 tcgaggtcga ggacgcccac ccgcttcacc ggcggggtct ggatctgctg gatgagcccg
      721 gccagatcga gctcgcggcc ctgccgccac gccgcgtcga ggatcgtcgc gatcaggatg
      781 tgctcgcggc tcttgatcgg atccgcgtcg atgccgagga ggcccagcag gctggtggcg
      841 gtcgtgccga tgcgctcgcg caggagctcc tcgtcctcgc ggatggcgga cggcggggcc
      901 gcgaacgact tcaggatcga gagcgggatg cccgcgttgc tgcccggggt gtagatcgcg
      961 aagtccgccg cgtcgcgcag gcgctggatg cgcgcgccgt cctctcccca ggccgcgagc
     1021 cccttctgcc actgctcggc ctgctgccgc gcgtagtcgt ccgcggagag gttcttccgg
     1081 cgggcgtcgt cctcgttgat ccagggccgg aaatcctcgg ggcggagcgc ggggaaggtg
     1141 agcagcaggt tggccaggtc gcccttcggg tcgatgagga tcgcggggat gccgtcgatg
     1201 gctgcttcct cgatcagggc caggcagagc ccggtcttgc cgctgccggt catgcccacg
     1261 cagaccgcgt gggtgaccag gtccttcgag tcgtagagca ggaccccgtc ctgcgccttc
     1321 ttctgctgcg ggtcgtaggg gcgacccagg tagaagacgc ccagcttctc gaagtcggcg
     1381 gcgctcggca tgcggagccc tccgtgtgag cctagatcgc gcgcttgacg aagacgccgc
     1441 ccttcacgat cgcgtgaaga ttggcctggt tctggaagac gcggaggttc ttgagcggat
     1501 tgccgtcgac caccagcagg tcggcgtact tgccgggctc caccgagccg atcttgtcgg
     1561 tcatccggaa gatctccgcg ttgaccatgg tggccgagag cagcacctcc atcggcttca
     1621 tgacctggcc cttcagctcg aactccatcg cgcgctgcgc ctgcatgtcg cccagcaggt
     1681 ccgagcccga gccgatcttg cagcccgcgc ggtaggcgtg ggtgaggccc tccaggctct
     1741 tctcgagcgc catgttgatc ttctggatct ggtggtcgcc gatcccgtag ttcttgccct
     1801 cgcgcgagat cgcctcgtag gtgaccatgg tcggcacgag gtacgcgccg gccgccttga
     1861 tcgcctgcgc gcccgcctcg tcgagcaggt tgccgtgctc gatgcagcgc accccggcct
     1921 ggatcgcgtt gcggatggcg gagccggagt aggcgtgggc cagcacgtac ttgcccaccg
     1981 cgcgcgcctc ctcgaccgcg gcgcgcatct cgtccaccgt gtactgcgtg gtgtcgagct
     2041 cgtcgcccgg cgacatcgcg ccgcccgagg ccatgatctt gatctggtcc acgtcgtggc
     2101 gcaggttctc gcgcgcggcc ttccgcacct cgtcggcccc gtccgcgatc atgcccgcca
     2161 tgcccacgca gcagcccacc ggctcgatcc actccgcgcg ccgccgcttg tcggcgtggc
     2221 cgccggtctg cgagagcggg cggccggaga ccagcatgcg cggccccggg tagaggccgg
     2281 tctcggccgc ctcgcggaag ccgtagtcgg cgccgcc
//
LOCUS       DATMCV010000901         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_846368, whole genome shotgun sequence.
ACCESSION   DATMCV010000901 DATMCV010000000
VERSION     DATMCV010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846368"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..922
                     /gene="rplB"
                     /locus_tag="VNU03_20600"
     CDS             98..922
                     /gene="rplB"
                     /locus_tag="VNU03_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWP76932.1"
                     /translation="MGIRTLKPTSPARRYMTQLTNEEITKKTPEKSLLSPKKRGSGRN
                     VYGRITVRHRGGGAKRMLRDVDFRREKVGIPAVVAGIEYDPGRSARIALLHYKDGEKR
                     YIIAPLGLKPGDMVMSGPQADILPGNALPIRNIPLGTLVHNVELQPGKGGQLCRSAGT
                     LAQLLAKEGDHANLKLPSGEVRQVKLECMATVGQVGNLDHENVSVGKAGRVRWKGFRP
                     TVRGTVMNPVDHPMGGGEGKGKGNHPMTPWGKPTKGYKTRRGARPSDRYIVTRRTK"
     gene            935..1228
                     /gene="rpsS"
                     /locus_tag="VNU03_20605"
     CDS             935..1228
                     /gene="rpsS"
                     /locus_tag="VNU03_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWP76933.1"
                     /translation="MGRSTRKGPYVEERLEKRVEELNRQRQKKVLKTWSRRSTIIPEF
                     VGHTVAVHNGRKFIPVYITENMVGHRLGEFALTRTFKAHGQAEKAATSPTGKS"
     gene            1231..1605
                     /gene="rplV"
                     /locus_tag="VNU03_20610"
     CDS             1231..1605
                     /gene="rplV"
                     /locus_tag="VNU03_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWP76934.1"
                     /translation="MRTRATARYIRVPASKARLVLDHIRGKSVGEALATLQYTPKAAG
                     RLIEKVLRSAIANAEHNHQVRNLDDLRVVKAIADGGPSMKRVSPRAMGRAYFIKHRTS
                     HLTIELSDETTASAAAARGGKR"
     gene            1608..2258
                     /gene="rpsC"
                     /locus_tag="VNU03_20615"
     CDS             1608..2258
                     /gene="rpsC"
                     /locus_tag="VNU03_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWP76935.1"
                     /translation="MGQKTHPIGFRLGSTRTWSSRWFATKGYAALLHEDHKIRRFIKS
                     SLYHAGISRIDIERSANRARITIFTARPGIIIGRKGAEVEKLKNEIQSRTAKEVYLNI
                     EEVVHPELDAQLVAENVALQLQKRVAFRRAMKKAVTSALRLGADGIRIACSGRLGGAE
                     IARREWYRDGRVPLHTIRADIDYGLAEAHTTFGAIGVKVWIFKGEVLPAQKAAAEG"
     gene            2265..>2592
                     /gene="rplP"
                     /locus_tag="VNU03_20620"
     CDS             2265..>2592
                     /gene="rplP"
                     /locus_tag="VNU03_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWP76936.1"
                     /translation="MLMPKRVKYRKAQRGRMKGKAQRGSTLAFGEFGLKALEPGWVTN
                     RQIEAARVSLARSVKRGGKIFIRIFPDKPVTKKPAETRMGKGKGNPEFWVAVVKPGRI
                     MYEMEGV"
BASE COUNT          494 a          860 c          917 g          321 t
ORIGIN      
        1 atgggcaagc acgaggggcg ccggccgagc tggaagaagg cggtggtgac gctccggccc
       61 ggccacaaga tcgagctggt cgagggagcc taggaccatg ggaatccgga cgctcaagcc
      121 cacctcgccg gcccggcgct acatgacgca gctgaccaac gaggaaatca cgaagaagac
      181 gccggagaag agcctgctca gtccgaagaa gcggggcagc ggccgcaacg tgtacgggcg
      241 gatcaccgtc cggcatcggg gcggcggggc caagcggatg ctccgcgacg tcgatttccg
      301 gcgcgagaag gtcggcatcc cggccgtggt ggcggggatc gagtacgatc cgggtcgctc
      361 ggcgcgcatc gcgctcctgc actacaagga cggcgagaag cgctacatca tcgcccctct
      421 cggcctcaag ccgggcgaca tggtcatgtc cgggccccag gctgacatcc tgcccggcaa
      481 tgccctgcct atcaggaata tccccctcgg cacgctcgtg cacaacgtcg agctgcagcc
      541 gggcaagggc ggccagctct gccggagcgc cggcaccctc gcccagctcc tggccaagga
      601 gggcgaccac gccaacctca agctgccctc gggcgaggtg cgccaggtca agctcgagtg
      661 catggcgacg gtgggccagg tgggcaacct ggaccacgaa aacgtgtccg tcggcaaggc
      721 aggccgcgtg cgctggaagg gcttcaggcc caccgtgcgg ggcaccgtga tgaatccggt
      781 cgaccacccc atgggcggcg gcgaaggcaa gggcaagggc aatcacccga tgactccgtg
      841 gggcaagccc accaagggat acaagacgcg gcgcggcgcc cgcccgtcgg accgctacat
      901 cgtgacgcgc cggacgaagt aggagccgag accaatggga cgctctacca ggaaagggcc
      961 gtacgtcgag gagaggctgg agaagcgggt ggaggagctc aaccgccagc gccagaagaa
     1021 ggtgctgaag acctggtcgc gccgctccac gatcatcccg gagttcgtcg gccacacggt
     1081 ggccgtgcac aacggccgga agttcatccc ggtctacatc accgaaaaca tggtgggcca
     1141 tcggctgggc gagttcgcat tgacacgcac gttcaaggcc cacgggcagg ccgagaaggc
     1201 cgccacctcg cccaccggga agtcctgagc atgcgcacgc gagcgaccgc gcgctacatc
     1261 cgggtgcccg cctccaaggc ccgcctcgtc ctggatcaca tccggggcaa gtcggtgggg
     1321 gaggccctgg ccacgctcca gtacaccccg aaggcggccg gccgcctcat cgagaaggtg
     1381 ctgcgctccg ccatcgccaa cgcggagcac aatcaccagg ttcgcaacct cgacgacctc
     1441 cgggtggtca aggcgatcgc ggacgggggt ccgtccatga agcgggtgtc cccgcgcgcg
     1501 atgggacgcg cctacttcat caagcaccgc acgagccacc tgaccatcga gctgagcgac
     1561 gagacgacgg cctccgccgc cgcggcgcga ggaggcaaga ggtagccatg ggccagaaga
     1621 cgcatccgat cgggttccgg ctggggtcca cgcggacgtg gagctcgcgc tggttcgcca
     1681 ccaagggata cgcggccctc ctgcacgagg accacaagat ccgccgcttc atcaagtcgt
     1741 cgctctacca cgcggggatc tcgcggatcg acatcgagcg ctcggccaac cgcgcccgca
     1801 tcacgatctt caccgcccgg cccggcatca tcatcggccg caagggggcg gaggtcgaga
     1861 agctgaagaa cgagatccag tcgcgcaccg ccaaggaggt ctacctcaac atcgaggagg
     1921 tggtccatcc cgagctggac gcgcagctgg tggccgagaa cgtggccctg cagctccaga
     1981 agcgggtggc attccgccgg gcgatgaaga aggcggtgac ctccgcgctg cgtctcgggg
     2041 ccgacggcat ccggatcgcc tgctcgggcc ggctgggcgg cgcggagatc gcgcggcgcg
     2101 agtggtatcg cgacgggcgg gtgccgctgc acaccatccg ggccgacatc gactacgggc
     2161 tggccgaggc ccacaccacg ttcggggcca tcggggtgaa ggtgtggatc ttcaagggtg
     2221 aggtgctccc cgcccagaaa gccgccgccg agggttaagc cgccatgctg atgcccaagc
     2281 gggtcaagta ccggaaggcc cagcgcggcc ggatgaaggg caaggcgcag cgcgggtcca
     2341 ccctcgcctt cggcgagttc gggctgaagg cgctcgagcc gggctgggtc accaaccgcc
     2401 agatcgaggc ggcccgggtg tcgctcgccc gcagcgtcaa gcggggcggc aagatcttca
     2461 tccgcatctt cccggacaag ccggtgacca agaagccggc cgagacgcgg atgggcaagg
     2521 gcaagggcaa cccggagttc tgggtcgcgg tggtcaagcc gggacggatc atgtacgaga
     2581 tggagggcgt gg
//
LOCUS       DATMCV010000902         3013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_47544, whole genome shotgun sequence.
ACCESSION   DATMCV010000902 DATMCV010000000
VERSION     DATMCV010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47544"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1557)
                     /gene="hemG"
                     /locus_tag="VNU03_20625"
     CDS             complement(136..1557)
                     /gene="hemG"
                     /locus_tag="VNU03_20625"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HWP76937.1"
                     /translation="MRLVVVGAGISGLAAAHRAVELAREQGRPLELTVLEGADRVGGT
                     IQTEQRDGFLVEGGPDSFLSEKPWALALCQRIGLEERLMRTDDRFRRTFVVWGGRLHP
                     LPEGFQLLAPTRLAPLLASGLFSWPGKLRMACDLVLPRGGDPDESLGAFVRRRLGREA
                     LERVAQPLVAGIYTADPDELSLSATMPRFVEMERRERSVILALWRAARKAPAQHAGTS
                     GARWSLFVTLARGMDELVQALAARLPPGAVRLKERVTGVARESGRWRIATASGVAYEA
                     DALVLASEAHQAARMLRYVDPALAHLLEGIPHASSATVSLAYRRADVRHPLDGFGFVV
                     PHAERRPIIACTFSSVKYAGRAPDGHVLLRVFLGGALNEGVLQGDDATLVRTAREQIG
                     PLLGITAEPELARVARHAQAMPQYHVGHAARAAAIEQAVSRHPGLRLVGGAYRGVGIA
                     DCVRSGEDAAERLLEETAGGSVG"
     gene            complement(1554..2480)
                     /gene="hemH"
                     /locus_tag="VNU03_20630"
     CDS             complement(1554..2480)
                     /gene="hemH"
                     /locus_tag="VNU03_20630"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWP76938.1"
                     /translation="MSAMDSVLLVAFGGPERSEDIRPFLQIVTTGRAISAARLEEVAH
                     HYEIIGGRSPLNALTRRQGDALASALAARERALPVWVGMRNWHPFLHETLAEMKDRGR
                     RRAVGIILSSLQTEASWARYMDDVAAAREKVGPDAPEVVFAPPWADHPRFVDAMAART
                     RAALEAVEPARRAAARLVFTAHSVPVAMATGSPYAAQLEAAGRAIAGRLGRARFTIAY
                     QSRSGSPRDPWLEPDVADAIRALGREGARDVVVVPVGFVCDHVEVLYDLDVEARRAAE
                     AAGVVFHRAPAANDHPDFIAMLADLVDRVTER"
     gene            complement(2477..>3013)
                     /locus_tag="VNU03_20635"
     CDS             complement(2477..>3013)
                     /locus_tag="VNU03_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWP76939.1"
                     /translation="AFMRQEPEAWAAMMTRLADVTADYLNGQIGAGVQAVQLFDSWVG
                     ALSPADYRAYVLPHSRRVMQALRPGVPVIHFGVGTGGLLPLMREAGGDVIGLDWRVEL
                     GSTWARLGHDVAVQGNLDPAVLLANVDAIRVGAREILDAAAGRPGHVFNLGHGIHKET
                     PVEHVRALVDIVHELSAR"
BASE COUNT          419 a         1173 c         1067 g          354 t
ORIGIN      
        1 cgaggacatc gactccgcgc gcggcgcgct cgccctcggc gcctccgact acgtcaccaa
       61 gccgttcagc ttccagtatc tcgactcggt gctcgaggtc cacatgctga tggaccacat
      121 cgacccgaag tcgagctagc caacactccc tcccgccgtc tcctccagca accgctcggc
      181 cgcgtcctcg cccgagcgta cgcagtccgc gattcccacc ccgcgatagg ctcctccgac
      241 caggcgcagg cccgggtgcc gcgacaccgc ctgctcgatc gccgccgcgc gggccgcgtg
      301 acccacgtgg tactgcggca tcgcctgcgc gtggcgggcc acccgcgcga gctcgggctc
      361 ggcggtgatg ccgaggagcg ggccgatctg ctcccgcgcg gtcctgacca gggtggcgtc
      421 gtcgccctgc agcacgccct cgttgagggc gccgcccagg aagacccgca gcagcacgtg
      481 cccgtcgggc gcgcgcccgg cgtacttcac gctcgagaag gtgcacgcga tgatcgggcg
      541 ccgctccgcg tgcgggacca cgaagccgaa gccgtcgagc gggtgccgca cgtccgcgcg
      601 gcggtaggcc aggctcaccg tcgcggacga ggcgtggggg atgccctcga ggaggtgggc
      661 gagcgccgga tccacgtacc gcagcatgcg cgcggcctgg tgcgcctcgg aggccaggac
      721 gagcgcgtcg gcctcgtagg cgacaccgct cgcggtcgcg atccgccacc gcccgctctc
      781 gcgcgccacc ccggtgacgc gctccttgag gcgcaccgct cccgggggga gccgcgcggc
      841 gagcgcctgg accagctcgt ccatgccccg ggcgagggtc acgaagagcg accagcgggc
      901 cccgctcgtc cccgcgtgct gcgccggcgc cttccgcgcc gcccgccaga gggccaggat
      961 cacgctccgc tcgcgccgct ccatctcgac gaagcgcggc atggtcgccg acagcgacag
     1021 ctcgtcggga tccgcggtgt agatgcccgc gaccagaggc tgggcgacgc gctcgagcgc
     1081 ctcgcggccg agtcgtcgcc gcacgaaggc gccgaggctc tcgtcgggat cgccgccccg
     1141 cggcagcacc aggtcgcagg ccatccggag cttgcccggc caggagaaga gccccgaggc
     1201 gagcagcggc gcgaggcggg tcggcgccag cagctgaaag ccctcgggca acggatgcag
     1261 gcggccgccc cagaccacga acgtgcgccg gaagcgatcg tcggtgcgca tcaaccgctc
     1321 ctccaggccg atccgctggc acagggcgag ggcccacggc ttctcggaca ggaaggagtc
     1381 gggcccgccc tcgacgagga agccgtcacg ctgctcggtc tggatggtgc cgccgacccg
     1441 gtccgcgccc tcgagcacgg tcagctcgag cggccgcccc tgctcccgcg caagctcgac
     1501 agcgcggtgg gccgcggcca gcccggagat ccccgcgccg acgacgacga gcctcaccgc
     1561 tcggtcactc gatcgaccag gtcggccagc atggcgatga agtcgggatg gtcgttggcg
     1621 gcgggtgcgc gatggaagac cacccccgcg gcctccgccg cccggcgcgc ctccacgtcg
     1681 aggtcgtaga gcacctccac gtggtcgcag acgaacccga ccggcaccac caccacgtcc
     1741 cgcgcgccct cgcggccgag cgcacggatc gcgtcggcga cgtcgggctc gagccacggg
     1801 tcgcgcggcg acccgctccg gctctggtag gcgatcgtga agcgcgcgcg gccgagccgc
     1861 cccgcgatcg cccgccccgc cgcctccagc tgcgccgcat acggcgagcc cgtggccatg
     1921 gccaccggca cgctgtgcgc ggtgaacacg aggcgcgcgg cggcccggcg tgcgggctcg
     1981 accgcctcca gcgccgcgcg agtgcgggcc gccatcgcgt ccacgaaccg cgggtgatcg
     2041 gcccacggcg gcgcgaagac gacctcgggc gcgtcggggc cgaccttctc ccgcgcggcg
     2101 gccacgtcgt ccatgtagcg ggcccacgag gcctcggtct gcagcgacga gaggatgatg
     2161 ccgaccgcgc ggcgccgacc ccgatccttc atctcggcga gggtctcgtg gagaaacggg
     2221 tgccagttcc gcatgccgac ccagaccggc agcgcgcgct cccgggcggc gagcgcgctg
     2281 gcgagcgcgt cgccctgccg gcgcgtgagg gcgttcagcg gcgagcgccc gccgatgatc
     2341 tcgtagtggt gggcgacctc ttccaggcgc gcggcggaga tcgcgcgccc ggtcgtgacg
     2401 atctggagga acgggcggat gtcctcggac cgctccggcc cgccgaaggc gacgagcagc
     2461 accgagtcca tcgcgctcat cgcgcggaca gctcgtggac gatgtcgacc agcgcccgca
     2521 cgtgctcgac cggcgtctcc ttgtggatgc cgtgcccgag gttgaagacg tgccccgggc
     2581 gccccgccgc cgcgtcgagg atctcgcgcg ccccgacccg gatcgcgtcg acgttcgcca
     2641 gcagcaccgc ggggtcgaga ttcccctgca ccgccacgtc gtgcccgagc cgcgcccagg
     2701 tagacccgag ctctacccgc cagtcgagcc cgatcacgtc gccgcccgcc tctcgcatca
     2761 gcggcagcag gccgccggta ccgacaccga agtggatgac cggaacgccc ggccgcaggg
     2821 cctgcatgac ccgtcggctg tgcggcagca cgtaggcccg atagtcggcc ggcgagagcg
     2881 cacccaccca cgagtcgaag agctgcaccg cctgcacgcc ggcgccgatc tggccgttca
     2941 gatagtcggc ggtgacgtcg gcgagtcgcg tcatcatggc ggcccacgcc tccggctcct
     3001 gccgcatgaa ggc
//
LOCUS       DATMCV010000903         4311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_846618, whole genome shotgun sequence.
ACCESSION   DATMCV010000903 DATMCV010000000
VERSION     DATMCV010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846618"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VNU03_20640"
     CDS             complement(<1..666)
                     /locus_tag="VNU03_20640"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP76940.1"
                     /translation="MRWKGWPLLPSLAFLLLLFVYPVGQLLWLSVVDRSGAFTGQHFA
                     RLFASSLYVDVLLITLKISAWATAFALLGGYPVAYLLATSDARWRSRLTFWVLLPFWT
                     SFLVRAFAWMVLLGRNGALNKLLEATGLTNAPVAVIFNLTGVLIGMTHALMPLGILTM
                     VAVMEHIDANLPKAAATLGARGGQTFWRIYFPLSLPGVAAAGLLVFISAVGFFIIPAL
                     LGGR"
     gene            complement(663..1721)
                     /locus_tag="VNU03_20645"
     CDS             complement(663..1721)
                     /locus_tag="VNU03_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP76941.1"
                     /translation="MVALAGADLELREGEFLTLLGPSGSGKTTLLMIVAGLIQPDGGE
                     VSIDGRLATYAPPFKRDVGMVFQNYALFPHLSVGENIAFPLRMRRVPEATIQQEVRRI
                     LEVVRLPDVGGRLPRALSGGQQQRIALARCMVYKPSIILMDEPLGALDKKLRDQMQLE
                     IKQLHAELRITVLYVTHDQEEAMIMSDRICLMRDGHIEQIGTPDELYFQPRTRFAAEF
                     LGESNVLVCEVVGVEASRVVVTTAGVRLRAPQDARLRDARRVALMVRPERLAIQDAPT
                     AGPGPEPVNRLEGHLRQVIRVSGITKYYVGLPSGQTVVASTLTGDAKTVATPGARVGL
                     TWPPDAGILLPETATTER"
     gene            complement(1788..2876)
                     /locus_tag="VNU03_20650"
     CDS             complement(1788..2876)
                     /locus_tag="VNU03_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76942.1"
                     /translation="MNTPASRIRPVSRRTFIKTATVAASVVGAPFVWRKASAQAKRIV
                     IRDSGGPFTPAFEQAFYKPFREATGIEAVGVASAHEPTSQIKSMVDTKTYTWDMAQLS
                     QAAAEQLVNDGGYLERHELEKHPDVAEIPPEYMTPYCVGDDVYSTILAYRTDAFKGRK
                     APVTWKDLLDLKGVPGRRSLRKHPFDTVEEALLADGVAKDKLYPCDVDRAFRVLDRVK
                     KDVVVWWTGGAQASQMLKTGEIDVIPTWAARAQTVIDEGGPVAISWDQNLWAMEGWCI
                     LKGGPKAELCREFIKFASNAKRMAAFAPFIPNGPTNPNAYKYIDPKRAPVLTTFPDNR
                     NRGIKLDHVFWAGTKDKVTERFNAWLIA"
     gene            complement(2945..3790)
                     /locus_tag="VNU03_20655"
     CDS             complement(2945..3790)
                     /locus_tag="VNU03_20655"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWP76943.1"
                     /translation="MDFRTIRYEQLGAVVRITANRPEVLNAQSRVMILELDEAFRAAS
                     NDDATRVIIVAGAGAHFSAGHDLGTPDELEDQKAHPVGPGLPENLKRLSELYLEATLR
                     WRDVPKPTIAQVQGYCIMGGLMLASCCDLIIAADDAQFADRMVRWGGAHVQYFSMPWD
                     LGPRKAKEYLFTGDYIDAATALDLGLVNRVVPRATLADATLELAGRIALQDPFALKLA
                     KASVNETMDIQGQRRALENSFKNYMLTIPHRQALGTYGAAARAKGVKDRIKTRDAKFG
                     DAAGG"
     gene            3935..>4311
                     /locus_tag="VNU03_20660"
     CDS             3935..>4311
                     /locus_tag="VNU03_20660"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76944.1"
                     /translation="MAGRLRDKVALITGAGSGIGAATAVVFAQEGAKVALADLRPAEL
                     ETVAAQVKSAGSEALVMVGDVTSRPDSQRFVDETVKRFGRADVLVNSAGVAPRNAPAD
                     WDWEKVWDFVMAVNMKGTFLMSRA"
BASE COUNT          671 a         1456 c         1513 g          671 t
ORIGIN      
        1 ccggccgccg agcagggccg ggatgatgaa gaagcccacc gcactgatga agaccaggag
       61 ccccgcggcg gccactcccg ggagcgagag ggggaagtag atccgccaga aggtctgccc
      121 gccgcgggcc ccgagggtgg cggcggcctt cgggagattg gcgtcgatgt gctccatcac
      181 cgccaccatc gtcaggatgc ccagcggcat gagggcgtgg gtcatgccga tgagcacccc
      241 ggtcaggttg aagatcaccg ccaccggcgc gttcgtgagc ccggtcgcct ccagcagctt
      301 gttgagcgcg ccgttgcggc cgaggagcac catccaggcg aacgcgcgaa cgagaaagct
      361 cgtccagaac ggcaggagca cccagaacgt cagccggctc cgccaccgcg catcgctggt
      421 ggccaggaga tacgcgaccg gatagccgcc gagcagggcg aacgcggtcg cccacgcgga
      481 gatcttgagc gtgatgagga gcacgtcgac gtagagggac gaggcgaaga ggcgcgcgaa
      541 gtgctggccg gtgaaagcgc cgctgcggtc gacgacgctc agccacagga gctgccccac
      601 cggatagacg aagagcagca gcaggaacgc gagtgaaggg agcagcggcc agcccttcca
      661 cctcatcgct cggtcgtcgc tgtctccgga agcaggatgc cggcgtccgg gggccacgtc
      721 aggccgacgc gcgcgccggg cgtcgccacc gtcttggcgt ccccggtgag cgtggaggcg
      781 acgaccgtct gcccgctggg aagccccacg tagtacttcg tgatgccgct gacgcgaatc
      841 acctggcgca ggtggccttc cagccgattg accggctccg gtcccggccc ggcggtcggc
      901 gcatcctgga tcgccaggcg ctccgggcgc accatgagcg ccacccgtcg ggcgtcgcgc
      961 aggcgcgcat cctggggggc ccgcagccgg acgccggccg tcgtcacgac cacgcgcgac
     1021 gcctcgacgc cgacgacctc gcagacgagg acgttggact ccccgaggaa ttcggccgcg
     1081 aaccgcgtcc ggggctggaa gtagagctcg tccggcgtcc cgatctgctc gatgtggccg
     1141 tcccgcatca ggcagatccg atccgacatg atcatcgcct cttcctgatc gtgcgtgacg
     1201 tagaggacgg tgatgcggag ctcggcgtgc agctgcttga tctcgagctg catctggtcg
     1261 cgcagcttct tgtccagcgc ccccaggggc tcgtccatca ggatgatcga gggcttgtag
     1321 accatgcacc gggccagcgc gatgcgctgc tgctggcccc cggacagcgc ccgcggcaga
     1381 cggccgccga catcgggcag gcgcaccacc tcgaggatgc gccgcacctc ctgctggatc
     1441 gtggcctcgg gcacccgccg catgcgcagg gggaacgcga tgttctcgcc gaccgagaga
     1501 tgcgggaaca gcgcgtagtt ctggaagacc atgccgacgt cccgcttgaa cggcggcgca
     1561 tacgtggcca gccgcccgtc gatcgagacc tcgccgccgt cgggctggat cagccccgcg
     1621 acgatcatca gcagcgtggt cttgccggat cccgacggcc cgaggagcgt gaggaactcg
     1681 ccctcgcgga gctcgaggtc agcccccgcg agcgcgacca ccggcccgta ggtcttccgc
     1741 agccctcgga cctggagctt gtacgtggtc gacgctctcg atccgactca ggcgatgagc
     1801 caggcgttga agcgctcggt gaccttgtcc ttcgtgccgg cccagaacac gtggtccagc
     1861 ttgatgcccc ggttgcggtt gtcggggaac gtggtcagga cgggggcccg cttgggatcg
     1921 atgtacttgt aggcgttggg attggtcggc ccgttcggaa tgaacggggc gaaggccgcc
     1981 atccgcttgg cgttcgacgc gaacttgatg aactcccggc agagctccgc cttggggccg
     2041 cccttgagga tgcaccagcc ttccatggcc cacaggttct gatcccagga gatggcaacc
     2101 ggcccgccct cgtcgatcac ggtctgcgcg cgcgcggccc acgtcgggat gacgtcgatc
     2161 tcccccgtct tgagcatctg cgacgcctgc gcgccgcccg tccaccacac gaccacgtcc
     2221 ttcttgacgc gatcgaggac cctgaacgcg cggtcgacgt cgcacggata gagcttgtcc
     2281 ttcgccactc cgtcggcgag cagcgcctcc tccaccgtgt cgaacgggtg cttgcgcagc
     2341 gaccgccggc ccgggactcc cttgaggtcc aggagatcct tccacgtgac cggggccttc
     2401 cggcccttga aggcgtccgt gcgatacgcg aggatcgtgg agtacacgtc gtcccccacg
     2461 cagtacggcg tcatgtactc cggcgggatc tccgcgacgt ccggatgctt ctcgagctcg
     2521 tgccgctcga gatagccccc gtcgttgacc agctgctcgg ccgcggcctg gctgagctgc
     2581 gccatgtccc aggtgtaggt cttggtgtcc accatgctct tgatctggct cgtcggctcg
     2641 tgcgcggagg ccacccccac tgcctcgatc ccggtggcct cccggaacgg cttgtagaac
     2701 gcctgctcga aggcgggcgt gaagggcccg cccgaatccc ggatcacgat gcgcttggcc
     2761 tgcgcgctcg ccttgcgcca gacgaacggc gcgccgacaa ccgaggcggc caccgtggcg
     2821 gtcttgatga aggtgcgccg cgacacgggt cggatgcgag aggcgggcgt gttcatgggg
     2881 atcctccggt gaatggtcgc gccgacacta taccggccga tgattcgggt caaggggtgc
     2941 ccggtcaccc acccgcggcg tccccgaact tcgcgtcgcg cgtcttgatc cggtccttca
     3001 cgcccttggc ccgcgccgcc gcgccgtagg tgccgagcgc ctggcggtgc ggaatcgtca
     3061 gcatgtagtt cttgaacgag ttttcgaggg cgcggcgctg gccctggatg tccatcgtct
     3121 cgttcaccga cgccttggcg agcttcagcg caaaaggatc ctgcagcgcg atgcgccccg
     3181 ccagctcgag cgtggcgtcg gccagtgtgg cgcgtggcac cacccggttg acgaggccca
     3241 gatcgagcgc cgtcgccgcg tcgatgtagt cgccggtgaa gaggtactcc ttggccttcc
     3301 gcgggccgag atcccacggc atgctgaagt actgcacgtg cgcgccgccc cagcgcacca
     3361 tccggtcggc gaactgcgcg tcgtcggcgg cgatgatgag gtcgcagcac gaggccagca
     3421 tgagcccgcc catgatgcag tagccctgca cctgggcgat ggtcggcttg ggcacgtcgc
     3481 gccagcgcaa agtcgcctcg aggtacagct cggacagccg cttgaggttc tcgggcaggc
     3541 ccgggccgac cgggtgggcc ttctggtcct cgagctcgtc cggggtgccg aggtcgtggc
     3601 ccgccgagaa gtgcgccccg gcgccggcca cgatgatgac gcgcgtcgcg tcgtcgttcg
     3661 acgccgcgcg gaatgcctcg tcgagctcga ggatcatcac gcggctctgg gcgttgagta
     3721 cctcgggccg gttcgcggtg atgcggacca cggctcccag ctgttcatag cggatggtgc
     3781 gaaagtccat cgagccctcc tggcactgaa aacgaccgcc ccacaatagc ccacgcgggc
     3841 gcgtcacgac cagccccctg gcggtgtgga cattgaccgg gggtgctggc ttgggtacgg
     3901 tgaagcaccc caaaacatcc atggaggtct ccagatggcg ggccggcttc gggacaaggt
     3961 agcgctgatc acgggtgcag gttcgggcat cggcgcggcg acggccgtgg tgttcgccca
     4021 agagggcgcg aaggtggcgc tcgccgacct gcggccggca gagctggaga ccgtcgcggc
     4081 ccaggtcaag agcgccggca gcgaggcgct ggtgatggtg ggtgacgtga cgagccggcc
     4141 cgactcgcag cggttcgtcg acgagacggt gaagcgattt ggccgggcgg acgtgctcgt
     4201 gaacagcgcc ggggtggccc cgcggaacgc gccggccgac tgggactggg agaaggtctg
     4261 ggacttcgtg atggccgtca acatgaaggg cacgtttctc atgtcccggg c
//
LOCUS       DATMCV010000904         2600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_782286, whole genome shotgun sequence.
ACCESSION   DATMCV010000904 DATMCV010000000
VERSION     DATMCV010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782286"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VNU03_20665"
     CDS             <1..276
                     /locus_tag="VNU03_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HWP76945.1"
                     /translation="DLTELAGQAIGKYHRYPDGLVLFIGTMFAPVADRGAPGSGFTHK
                     WGDTVSIAAPEIGRLVNEVVACDVAEAWSFGTGALMTSLARRGLLRA"
     gene            complement(179..844)
                     /locus_tag="VNU03_20670"
     CDS             complement(179..844)
                     /locus_tag="VNU03_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP76946.1"
                     /translation="MIKPIRRVVTGHNAQGRSVIASDGPSPHVLTLPGRPDFALTDLW
                     VSDRAPASNAGSADPAKRRMSLEPPANGTIFRIVEFPPDAAAGGGFDRAAAFRAMGAS
                     HAMDPDASRHPAMHRTDTVDYALVLSGEIWALMDEGETLLRAGDTLVQRGTNHSWSNR
                     GDQPSLVAFVLVSATSLGDKPGGKAAAKPVRRAAARAGRGSSSRRPSRRTTPRPRRKP
                     RPR"
     gene            932..>2600
                     /locus_tag="VNU03_20675"
     CDS             932..>2600
                     /locus_tag="VNU03_20675"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP76947.1"
                     /translation="MPDLVIRGGTVVDGSGTPGRRADVAIQDGRIGAVGEVRDRGARE
                     IDAGGLVVAPGFIDVHTHYDAQYTWDPYATSSIWHGVTTTVIGNCGFAIAPCRPADRD
                     LIMRTLVKVEGMSLAAMRAGITWGFETFPEYLDHLDRCNPSLNVAALVGHSTIRQWVM
                     GKDSQHRVATAEEVAAMCDLVREAMAAGAIGLGSSTAEAHVGDGGLPVASRLADKAEF
                     LALTKAMGESGRGLFQITIGPKTTMEDLRDIWRTSGRPAIWAAFFQRDDRPEYVPERL
                     AITEAFHREGVEVLPQISCRPLTMDFTMKNPYPFEGMPAWQQVMKRPESEWLGVYGDP
                     GFRAALKADLDARRLAVFRGRWDLVQVLRTHRPEHKALEGRSLADLARETGKHPVDAW
                     LDLVTADGLDVEFLAGLLNTDEQVVGDLIVHPLTLITLSDAGAHLSLMCDAGYSSTLL
                     GKWVRSLRRLPLEAAVKRLTDDPARAYRIPGRGRLAPGYWADVAIFDPATVDALPVEW
                     VSDLPAGEPRFVSHARGVEWSLVNGRPVLERGRVVEQPAGARPGRVLR"
BASE COUNT          349 a          932 c          961 g          358 t
ORIGIN      
        1 gacctgaccg agctggccgg ccaggccatc gggaaatacc accgctaccc ggacggcctc
       61 gtgctcttca tcggcacgat gttcgcgccg gtggccgacc gcggagcgcc gggcagcggc
      121 ttcacccaca agtggggcga caccgtgtcg atcgccgcgc ccgagatcgg gcgcctcgtc
      181 aacgaggtcg tggcttgcga cgtggccgag gcgtggtcct tcgggacggg cgccttgatg
      241 acgagcctcg cccggcgcgg gctgctgcgc gcctgaccgg cttcgcggcg gccttgccgc
      301 ccggcttgtc gccgaggctc gtcgcgctca ccagcacgaa ggcgaccagg gacggctgat
      361 cgccccgatt gctccaggag tggttggtcc cgcgctgcac cagggtgtcg cccgcccgca
      421 gcagggtctc gccctcgtcc atcagggccc agatctcccc cgacagcacg agcgcgtagt
      481 ccaccgtatc ggtgcggtgc atggcgggat gccgcgaggc gtccggatcc atcgcgtggc
      541 tggcgcccat cgcgcggaag gcggccgcgc ggtcgaagcc gccccccgcc gccgcgtcgg
      601 gcgggaactc gacgatccgg aagatcgtgc cattggccgg cggctcgagc gacatccggc
      661 gcttcgccgg gtccgcgctc cccgcgttgg aggcgggcgc gcggtcgctc acccagagat
      721 cggtcagcgc gaagtcgggg cggcccggca gcgtgagcac gtggggcgac gggccgtcgc
      781 tcgcgatcac cgagcggccc tgggcgttgt gcccggtcac gacgcgtcga atcggcttga
      841 tcatggcagc ctcccggccg ggactataca ccgcgctgtc gggtaggtga aactggggcg
      901 ccggtaccct gcagtcggaa agaggcgcga catgcccgat ctggtgattc gcggcggcac
      961 cgtggtggac ggcagcggca ctccgggccg gcgggccgac gtggcgatcc aggatgggcg
     1021 gatcggcgcg gtcggtgagg tgcgcgaccg tggcgcgcgc gagatcgacg cgggcgggct
     1081 ggtcgtcgcg cccggcttca tcgacgtcca cacccactac gacgcccagt acacgtggga
     1141 cccgtacgcc accagcagca tctggcacgg ggtcaccacc accgtgatcg gcaactgcgg
     1201 cttcgccatc gcgccgtgcc ggcccgccga ccgggacctg atcatgcgca cgctcgtgaa
     1261 ggtggagggc atgtcgctcg ccgcgatgcg cgcgggcatc acgtggggct tcgagacctt
     1321 ccccgagtac ctggaccacc tcgaccgctg caacccgagc ctcaacgtgg cggcgctcgt
     1381 cggccactcg acgatccggc agtgggtgat gggcaaggac agccagcacc gcgtggcgac
     1441 cgcggaggag gtcgcggcca tgtgcgacct cgtgcgcgag gcgatggcgg cgggcgcgat
     1501 cggcctcggg tcgtccaccg cggaggcgca cgtgggcgac ggtggcctgc cggtggcgag
     1561 ccggctcgcc gacaaggcgg aattcctcgc gctgaccaag gcgatgggcg agtcggggcg
     1621 cgggctgttc cagatcacca tcgggcccaa gaccaccatg gaggacctcc gcgacatctg
     1681 gcgcacgagc ggccgccctg cgatctgggc cgcgttcttc cagcgcgacg accggcccga
     1741 gtacgtgccg gagcgcctcg cgatcaccga ggcgttccac cgcgagggcg tggaggtgct
     1801 gccgcagatc tcctgccggc cgctcacgat ggacttcacg atgaagaacc cctacccgtt
     1861 cgagggcatg ccggcctggc agcaggtcat gaagcggccc gaatcggagt ggctcggggt
     1921 atacggggat ccgggcttcc gcgccgccct caaggccgat ctcgacgcgc gccggctcgc
     1981 ggtcttccgc ggccgctggg acctggtcca ggtgctgcgc acccaccgtc cggagcacaa
     2041 ggccctcgag ggccggagcc tggccgacct cgcgcgcgag accggcaagc acccggtgga
     2101 cgcgtggctc gacctcgtga ccgccgacgg tctcgacgtg gagttcctgg ccggcctgct
     2161 caacaccgac gagcaggtgg tgggcgatct gatcgtccac ccgctcacct tgatcacgct
     2221 ctccgacgcg ggcgcgcacc tctcgctgat gtgcgacgcg ggctactcga gcacgctgct
     2281 cggcaagtgg gtgcggagcc tccggcgcct gccgctcgag gccgcggtca agcgcctcac
     2341 cgatgatccc gcgcgcgcgt accgcatccc cgggcgcggg cggctcgcgc ccggctactg
     2401 ggccgacgtg gcgatcttcg atccggccac cgtcgacgcg ctgccggtcg agtgggtgag
     2461 cgacctgccc gcgggcgagc cgcgcttcgt ctcccacgcg cgcggcgtgg agtggagcct
     2521 ggtcaacggc cggccggtgc tcgagcgcgg ccgcgtggtc gagcagccgg ccggggcgcg
     2581 cccgggccgg gtgctgcgcc
//
LOCUS       DATMCV010000905         5539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_47781, whole genome shotgun sequence.
ACCESSION   DATMCV010000905 DATMCV010000000
VERSION     DATMCV010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47781"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..818)
                     /locus_tag="VNU03_20680"
     CDS             complement(198..818)
                     /locus_tag="VNU03_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76948.1"
                     /translation="MKYKVLKSAAHNFGRSFASSLNWRGDDYVMSHLARAVVTSGEVE
                     LSVDLLSGKAQPTPLLEEPVQTSVMAYVRWFPDLLRSQRVTPEAVRAASMRVRFQPER
                     RVDLHGEMGGWTIPFECVVSLTDDRGKVHEGRVIDDWAVDDFNIPPSWRRRLRWWLSD
                     LRRSWHQYRFRQWGMWCQIRSSRSERIPPNIRFQGTALRAAAEPGR"
     gene            1551..2189
                     /locus_tag="VNU03_20685"
     CDS             1551..2189
                     /locus_tag="VNU03_20685"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWP76949.1"
                     /translation="MDVSAVARWRVVCLLMLGAFLGLSGVVYAVGLLPGDVAVHGEIL
                     EARGTLAHAAARWFDYGGKWVVLGPAMLAVFAWSPIARRHWWLWCALMPVGGAIEQLF
                     KFLVGRPRPRGVHWGFPSGHVTAATTFAVLLLYALSREPASPRARVGLIVLAVALVGT
                     VGYARVILNAHWPSDVLGGVLLGGGCAAAGAWWDSLHGATPRAVDDAVRVQG"
     gene            2190..2933
                     /locus_tag="VNU03_20690"
     CDS             2190..2933
                     /locus_tag="VNU03_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWP76950.1"
                     /translation="MRPFVELEDVDFAYGAVSVLERINLTVEAGDFLGIIGPNGSGKT
                     TLLRIVLGLLPPTRGTVRLFGHPPASFRQWGRLGYVPQKATLDPALPVTVREVVATGL
                     LPSLGLFGRLGPAARQRIDEVLGQVGMADHASARIGALSTGQQQRVLIARALVSKPEL
                     LILDEPTGGVDPEAQTSFYALLHHLNRERDVTLILVSHDIGVVAKEVTKLACLNRRLI
                     FHGRPGDFLSDAALTALYGPAVRVVSHDH"
     gene            2937..3764
                     /locus_tag="VNU03_20695"
     CDS             2937..3764
                     /locus_tag="VNU03_20695"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWP76951.1"
                     /translation="MPEFLQYGFMHRAFAAGVVTALICPAIGVFLIPRRLSLIADTLA
                     HVALAGVALGLVLGISPVIGALVVVLAGAVGMERLRARGALQGDAALAVFLSGGFALA
                     VVLISLARGFNADLFAILFGSILTVTPGDVWLILALGATVTVAIGAFYRQLLAITLNE
                     DLARTSGVPVGALNLLLTVLTALTTVVAMRMVGVLLVSAMIVIPTLAGFALGRSFHTA
                     TAIAVGIALLSVISGLIAAYYLRLAAGGAVVLVALLIFAAVSLLRRRIRPSIREVHP"
     gene            3761..4279
                     /locus_tag="VNU03_20700"
     CDS             3761..4279
                     /locus_tag="VNU03_20700"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWP76952.1"
                     /translation="MIRPRAALLAATFLLSALAPAATAQSPTAKAAPNPECPRWRDAF
                     ATMPLRMVSIQAGTRALALRVKMADSGERQAGGFQCATPEEIKRHLILFDFGKEIFTQ
                     FHMQNVPAALDIAFIKNDGRIFAILKMDPSPTELYGPLGEFRYALEAHAGFFASQGIR
                     QGEAKLVVPATR"
     gene            4291..5190
                     /locus_tag="VNU03_20705"
     CDS             4291..5190
                     /locus_tag="VNU03_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWP76953.1"
                     /translation="MHAHHRHTHGSRHKHRLLAVFGLTAGFLVVEAGVGLWTGSLSLL
                     ADAVHMLVDAGGVLLSLLAVWFAERPATPAKTYGYYRVEILAALVNGVLLCVMAVGIL
                     VATYGRLWQPPHVPGGPILAVALLGLLVNLAGLWLLHGGAGESLNVRSAYLEVMGDAI
                     SSGVVIVAGVVILSTGWTIADPIAGALIALFLLPRTWALLRQTVNVLLEGAPAHLDVT
                     EIEEALCSAPSVRRVHDLHVWTLTSGREAMSVHVVVEAGAPSDKILEALHVILHARFG
                     IDHTTIQIETEPAPLIQITPKAG"
BASE COUNT          778 a         2012 c         1834 g          915 t
ORIGIN      
        1 tccgcatggc agcgtagaca gaggcaaatg gcgttctcaa gcgtattaga accgccgtgt
       61 gcctcttgaa cgatgtgatg aacgttggag gcgcggccag caaactgatg gcagacacag
      121 cagtaccgtc gggaccgtac aagtgcttgg atgcggacgg agtcggggaa cggcatatgg
      181 gcggtccttt gtgcggccta acgtccaggt tcagcggcgg cgcgaagcgc cgtcccttga
      241 aacctgatgt taggcggaat gcgctcagat ctgctgctgc ggatctggca ccacatcccc
      301 cactgcctga accgatactg atgccaagac cgccgcagat cagaaagcca ccaccgtaga
      361 cgccgccgcc aactcggcgg gatattaaaa tcatccaccg cccagtcgtc gatcacgcgg
      421 ccttcatgga ctttgccgcg gtcgtcggtc aggctcacga cgcactcaaa agggatcgtc
      481 cagccaccca tctccccgtg caaatctacc cgccgctcgg gctgaaaccg cacgcgcatg
      541 cttgccgctc gtactgcctc gggcgtgaca cgctggctgc gcagcagatc agggaaccag
      601 cggacatagg ccatcacgga cgtctggact ggctcctcga gaagcggagt aggttgcgcc
      661 ttgccggaca gcagatccac gctgagttcg acttcgcctg aagtgacgac cgcccgggcc
      721 aggtgactca tgacatagtc gtcgccgcgc cagttcaggg agctggcaaa ggagcggccg
      781 aagttgtggg ctgcactctt tagcaccttg tacttcattg cgcctcgcgg ccgcctaacg
      841 gtcgcgctca gtggccgggc gcgagcaacg cgagcggccg gtccactgca gcgtggggtt
      901 agaccacatc gcgcgacgcg ttgcctagta gaggcgcgat gtgcaagaca agcgtaggca
      961 aggactcagc cagatgctcg ggcgagaaca tgttcccagc aaagcccggg tgcgatgagc
     1021 ggccgaaaac ctctgtgtca ccagccctga ccgtgaactc ccaatcgcca taggcaacct
     1081 cccacgcgct catgacgtca gcgaaccgta tgacaaagcc cgctcggaac tgatccggtc
     1141 gcccgagtag ccgacgtgac tcaacgcgaa ggcctcgcct ggccagtgag ccgaacaggt
     1201 cctccgtctg ctgcgcgagt tcttcaatcg aaggcatggc cgatgtggtc taactaaaat
     1261 tagacaggtc tggataattc gtggacactg cgagcccggt cagtctaatc tgaccgggtg
     1321 tccggtgggt gtggcgggag cggccttcta ggacgcacgt ttgacgcatg gtccctggct
     1381 accctgccag tgttgcacgg accgggctat ttcggtgtgg atcccgccat gtgctgggcc
     1441 ttcgtcggtc gccccgcgac gtgacacccc gtcttcgggg cgggcgaagc ggcgcgcgat
     1501 ggtttagcag accccgcccc ttcgacccgt tcgagtacaa tgtgagcgcc atggacgtat
     1561 ccgccgtcgc gcgctggcgc gtggtctgcc tgctgatgct gggcgccttc ctcggactga
     1621 gcggggtcgt ctacgcggtg ggcctcttgc cgggcgacgt ggcggtccac ggcgagatcc
     1681 tggaggcgcg cggcacgctc gcgcatgcgg ccgcccgctg gttcgactac ggcggcaagt
     1741 gggtggtcct cgggccggcc atgctggccg tcttcgcgtg gtcacccatc gcgcggcggc
     1801 actggtggct ctggtgcgcg ctcatgccgg tcggcggcgc catcgagcag ctcttcaagt
     1861 tcctggtcgg ccggccccgg ccccgcggcg tccactgggg gttcccgagc ggccacgtca
     1921 ccgcggccac gaccttcgcg gtgctcctgc tctacgcgct ctcgcgcgag ccggcctccc
     1981 cgcgggcccg cgtgggcctg atcgtgctgg cggtggccct ggtcggcacg gtgggctacg
     2041 cccgggtcat cctcaacgcg cactggccct cggacgtcct gggcggcgtg ctcctcggcg
     2101 gcggctgcgc ggccgccggc gcgtggtggg acagcctcca cggggcgacg ccgcgcgcgg
     2161 tcgacgacgc ggtccgggtg cagggctaga tgcgcccgtt cgtcgagctg gaggacgtcg
     2221 acttcgccta cggcgcggtg tcggtcctcg agcggatcaa cctgacggtg gaggcgggcg
     2281 acttcctggg catcatcggc cccaacgggt ccggcaagac cacgctcctc cgcatcgtgc
     2341 tcgggctgct cccgccgacc cgcgggacgg tccgcctctt cggccacccg ccggcctcct
     2401 tccgccagtg ggggcgactg ggctacgtgc cccagaaggc cacgctggat cccgcgctgc
     2461 ccgtgacggt gcgcgaggtg gtcgccaccg ggctcctgcc ctcgctcggg ctcttcggcc
     2521 gcctcggccc cgcggcccgc cagcgcatcg acgaggtgct gggccaggtg ggcatggccg
     2581 atcacgcgtc cgcgcggatc ggcgcgctct cgactgggca gcagcagcgg gtgctcatcg
     2641 cgcgcgcgct cgtgtccaag cccgagctgc tgatcctgga cgagcccacc ggcggcgtgg
     2701 atccggaggc ccagacgagc ttctacgcgc tgctccacca cctgaaccgg gagcgcgacg
     2761 tgaccctgat cctggtgagc cacgacatcg gcgtggtggc caaggaggtc accaagctcg
     2821 cctgcctcaa ccgccgcctg atcttccacg gccgcccggg cgacttcctg agcgacgcgg
     2881 ccctgaccgc gctctacggc cccgcggtgc gcgtggtcag ccacgaccac tagctcatgc
     2941 ccgagttcct ccagtacggc ttcatgcacc gggccttcgc ggccggcgtg gtcaccgcgc
     3001 tcatctgccc ggccatcggc gtcttcctga tcccgcgccg gctctccctc atcgccgaca
     3061 ccctcgcgca cgtggccctg gccggggtgg cgctcggcct ggtgctgggg atctcgccgg
     3121 tgatcggcgc gctcgtggtg gtgctggccg gcgcggtggg gatggagcgg ctgcgcgcgc
     3181 gcggggcgct ccagggcgac gcggcgctcg cggtcttcct ctccggcggc ttcgccctcg
     3241 cggtggtgct catcagcctg gcccgcggct tcaacgccga tctcttcgcg atcctgttcg
     3301 gcagcatcct caccgtcacg ccgggcgacg tctggctcat cctggccctg ggcgcgacgg
     3361 tgaccgtcgc gatcggcgcc ttctaccgcc agctcctcgc gatcaccctc aacgaggacc
     3421 tggcccgcac cagcggggtg ccggtcggtg ccctcaacct gctcctcacc gtgctcactg
     3481 cgctcaccac ggtggtcgcg atgcggatgg tcggcgtgct gctggtgtcg gccatgatcg
     3541 tgatccccac cctggccggc ttcgcgctgg ggcggagctt ccacaccgcc accgcgatcg
     3601 cggtcggcat cgcgctgctc tcggtgatct cgggcctcat cgcggcctac tacctgcggc
     3661 tggccgcggg cggcgcggtg gtgctcgtcg cgctcctgat cttcgccgcg gtgagcctgc
     3721 tccgccgtcg gatccggccg tccatccgtg aggtccatcc atgatccggc cccgagccgc
     3781 cctcctggcg gcgacgtttc tgctatccgc gctcgcgccg gccgccaccg cccagagccc
     3841 gacggccaag gcggccccga accccgagtg cccgcgctgg cgcgacgcct tcgcgaccat
     3901 gccgctgcgc atggtctcca tccaggccgg cacccgcgcc ctcgccctgc gggtgaagat
     3961 ggccgacagc ggggagcggc aggcgggcgg cttccagtgc gcgacgcccg aggagatcaa
     4021 gcggcacctg atcctcttcg atttcgggaa ggagatcttc acccagttcc acatgcagaa
     4081 cgtcccggcc gcgctcgaca tcgccttcat caagaacgac gggcgcatct tcgcgatcct
     4141 caagatggac ccgagcccga ccgagctgta cggtcccctc ggggagttcc gctatgccct
     4201 cgaggcgcac gccgggttct tcgcgagcca gggcatccgg cagggagaag ccaagctggt
     4261 cgtgcccgcg acccgctgag cgcgaccgcc gtgcacgcgc accatcgcca cacccacggc
     4321 tcccgccaca agcaccgcct cctcgcggtg ttcggcctca ccgcgggctt cctggtggtg
     4381 gaggccggcg tcggcctgtg gaccggcagc ctctcgctcc tggccgacgc ggtgcacatg
     4441 ctggtcgacg cgggcggcgt cctcctgagc ctgctcgcgg tctggttcgc ggagcggccc
     4501 gccactccgg ccaagaccta cggctactac cgcgtcgaga tcctcgccgc tctcgtcaac
     4561 ggcgtgctcc tgtgcgtgat ggcggtcggc atcctggtcg cgacctacgg gcggctctgg
     4621 cagccgcccc acgtgccggg cgggcctatc ctggccgtcg cgctgctcgg cctcctggtc
     4681 aacctggccg ggctctggct cctgcacgga ggcgccgggg agagcctcaa cgtgcgcagc
     4741 gcctacctcg aggtgatggg cgacgcgatc agctccggcg tcgtcatcgt ggccggggtg
     4801 gtcatcctct ccaccggctg gacgatcgcg gacccgatcg cgggcgcctt gatcgcgctc
     4861 ttcctgctgc cccgcacctg ggccctgctc cgccagaccg tcaacgtgct gctggagggc
     4921 gcgccggccc acctcgacgt gaccgagatc gaggaggcgc tctgcagcgc cccctcggtg
     4981 cgccgggtcc acgacctgca cgtgtggacg ctcacctccg gccgtgaggc catgagcgtc
     5041 cacgtggtgg tcgaggccgg tgcaccctcc gacaagatcc tggaagccct gcacgtcatc
     5101 ctccacgcca gattcggcat cgatcacacc accatccaga tcgagaccga gccagcccct
     5161 ctcatccaga tcaccccgaa ggcgggctga tggctcgccc gcgcctggag aacccgatat
     5221 gcgacagatc gctcggctgg cttcgctcgc atcgctcctc gtcaccctca cctccaccgc
     5281 cgcgtgggcc gcggacgcac cggtcacggt gaccttcgcc caccccgaga agtacaccga
     5341 cctccgtctg agctgcgtct cccgcgacac ggatgcccgg agcctcatgg cggacctcga
     5401 gggccttcct caccgcgacc gccgcgccgc tcgtgcccgc gggccatcgg ctcgagatca
     5461 cggtgaccaa cgtcgacatg gcgggcgaca tcgagagctg gcgcggaccg gggcgctgcg
     5521 acgtgcgggt catgaagga
//
LOCUS       DATMCV010000906         3909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_388186, whole genome shotgun sequence.
ACCESSION   DATMCV010000906 DATMCV010000000
VERSION     DATMCV010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388186"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2607)
                     /locus_tag="VNU03_20710"
     CDS             complement(<1..2607)
                     /locus_tag="VNU03_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HWP76954.1"
                     /translation="MHEDVSAMAPGLPPAQGLYDPAHEHDACGVGFVVDIKGRKSHGI
                     VSQALTVLKNLLHRGACGCEVNTGDGAGILIQMPHLFLTRECAALGIALPEPGRYGAG
                     LVFLPRDTRDRAACRAILERIVVEEGQVVLGWRPVPTDSSPIGPSARSVEPTFEQVFV
                     ARGAGVPDRAAFERKLYIIRKRAEHEVRKSAIGARQYFYLPSLSANTLIYKGMLSADQ
                     IEGMFPDVTDPLVESALALVHQRFSTNTFPSWPLAHPYRFIAHNGEINTLRGNINWMR
                     AREALCHSAVLGDDLKKIVPIVVEGGSDSAVFDNVLEFLVMAGRPLPLAVLMMIPEAW
                     SGHESMSEERKAFYEYHGCLMEPWDGPASIAFTDGTVIGAVLDRNGLRPSRYYVTKDD
                     MVVMASEVGVLDIAPERVLLKERLHPGRIFLVDTAQGRIIDDAELKHTYATQHPYGEW
                     IRAYLTPVENLPAPPSVPEPDHQTVLERQQVFGYTHEDLRLLLGPMAAGGEEPVGSMG
                     TDTSLAVLSERPRLLYDYFKQLFAQVTNPPLDGIREELVTQIATSIGPEGNLLEPVPS
                     ACRQIKLRTPILHNEELARIRHVDQPGFKSTTVSMLFPVADGSQGLERAMDELCRQVS
                     RAVADGCTFLILSDRGVDAAHAPIPALLATAGIHHHLIREGTRCKVGFVIETGEAREV
                     HHMALLLGYGAGAINPYLAFESLDDMIRQGLLPGLDHKTAVKNYIKALNKGVLKVISK
                     MGISTIHSYRGAQIFEAIGLGKPLIDRYFTWTASRISGVGIDLVAAEALARHRRAYPE
                     RPVGGRELSWGGEYQWRRDGEYHLFNPETVYKLQHATQSGQYRIFKEYTALVDDQNQS
                     RATLRGLFDLRYA"
     gene            2767..3660
                     /locus_tag="VNU03_20715"
     CDS             2767..3660
                     /locus_tag="VNU03_20715"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWP76955.1"
                     /translation="MNLETLKLYCDVVRLRSFSRGAAANAVSQSAASQAIQQLEAEVD
                     AVLLDRSRRPLLPTEQGQAFYDSCRTLLQGWEKARAELAAARERVEGTVRVAAIYSVG
                     LHDMSRHMQPFMSAYPQARVLLECLHPHKVVEAVLEDEADVGILSYPAATRALTVLPL
                     RMEPMVLVAHPAHRLARRRTVSPEELQGEKFVAFDRDLAIRKAIDRALRQHGVKVEVV
                     MEFDNVETIKQAIGINAGVSILPRPTVLNESGMRTLVAAPLGLPSLVRPIGIIHRRGR
                     RLTPVVARFIEQLQKPVDAAP"
     gene            3721..>3909
                     /locus_tag="VNU03_20720"
     CDS             3721..>3909
                     /locus_tag="VNU03_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HWP76956.1"
                     /translation="MSETPASLIIAASESDSNLAYACRFLAPDPFVFLQVNGRKTLLM
                     SDLEVDRARAQARVDEVLS"
BASE COUNT          593 a         1307 c         1407 g          602 t
ORIGIN      
        1 cgcgtagcgg aggtcgaaga gcccgcgcaa ggtggcccgg ctctggttct gatcgtcgac
       61 gagggcggtg tactccttga agatccggta ctggccgctc tgagtggcgt gctgcagctt
      121 gtacaccgtc tccgggttga agaggtggta ctcgccgtcg cgccgccact ggtactcgcc
      181 gccccaggag agctcgcggc cgcccaccgg ccgctccggg tacgcccgcc ggtgccgggc
      241 gagcgcctcc gccgcgacga gatcgatccc gaccccgctg atgcgcgacg cggtccaggt
      301 gaagtagcgg tcgatgagcg gcttgccgag cccgatggcc tcgaagatct gggccccccg
      361 gtaggagtgg atggtggaga tccccatctt ggagatcacc ttcagcacgc ccttgttcag
      421 cgccttgatg tagttcttga ccgcggtctt gtgatcgagc ccgggcagga ggccctgccg
      481 gatcatgtcg tcgaggctct cgaaggccag gtacggattg atcgcgcccg ccccgtagcc
      541 cagcagcagg gccatgtggt ggacctcgcg cgcctcgccg gtctcgatca cgaagccgac
      601 cttgcagcgc gtcccctcgc ggatgagatg gtgatggatg ccggcggtgg cgagcagcgc
      661 cgggatcggg gcgtgggccg cgtcgacccc gcgatcggac aggatcagga aggtgcagcc
      721 atccgcgacc gcgcggctca cctggcggca gagctcgtcc atcgcccgct cgagcccctg
      781 ggagccgtcc gcgaccggga agagcatgga gacggtggtg gacttgaagc cgggctggtc
      841 cacgtggcgg atgcgcgcca gctcctcgtt gtggaggatc ggggtcctga gcttgatctg
      901 gcggcacgcc gagggcaccg gctcgagcag gttgccctcg gggccgatgc tggtggcgat
      961 ctgggtgacc agctcctcgc ggatgccgtc gagcggggga ttggtcacct gcgcgaagag
     1021 ctgcttgaag tagtcgtaga gcaggcgcgg acgctccgag agcaccgcga gcgaggtgtc
     1081 ggtgcccatg gagcccaccg gctcctcgcc ccccgcggcc atgggaccca gcagcaggcg
     1141 aagatcctcg tgggtgtagc cgaacacctg ctgccgctcg agcaccgtct ggtgatcggg
     1201 ctccggcacg ctgggcggcg cgggcaggtt ctcgaccggg gtcaggtagg cgcggatcca
     1261 ctcgccgtac ggatgctggg tcgcgtaggt gtgcttgagc tcggcgtcgt cgatgatgcg
     1321 gccctgggcg gtgtcgacca ggaagatgcg gcccgggtgc agccgctcct tcaggagcac
     1381 ccgctcgggg gcgatgtcga gcaccccgac ctcggaggcc atcaccacca tgtcgtcctt
     1441 ggtgacgtag tagcgcgagg gccggaggcc gttgcggtcg agcaccgcgc cgatcacggt
     1501 gccgtcggtg aaggcgatcg aggcgggccc gtcccagggc tccatcaggc agccgtggta
     1561 ctcgtagaag gccttgcgtt cctcgctcat cgactcgtga ccgctccacg cctccgggat
     1621 catcatgagc acggccaggg gcaggggccg cccggccatg accaggaact cgagcacgtt
     1681 gtcgaagacc gcggagtcgc tgccgccctc caccacgatc ggcacgatct tcttgaggtc
     1741 gtcgcccagg accgcggagt ggcacagggc ctcgcgggcc cgcatccagt tgatgttgcc
     1801 gcggagggtg ttgatctccc cgttgtgggc gatgaagcga tacggatgcg cgagcggcca
     1861 cgacgggaag gtgttggtgg agaagcgctg gtggacgagg gccagcgccg actccaccag
     1921 gggatcggtc acgtccggga acatgccctc gatctggtcg gcggagagca tgcccttgta
     1981 gatgagggtg ttggccgaga ggctcgggag gtagaagtac tgcctcgcgc cgatcgcgct
     2041 cttgcgcacc tcgtgctcgg cgcgcttgcg gatgatgtag agcttccgct cgaaggcggc
     2101 gcggtcgggc acgcccgccc cgcgcgcgac gaacacctgc tcgaaggtgg gttccaccga
     2161 ccgggcgctc gggccgatgg gggaggagtc ggtggggacg ggccgccagc ccagcaccac
     2221 ctggccctcc tcgaccacga tgcgctcgag gatggcccgg cacgcggcgc ggtcgcgggt
     2281 gtcgcggggc aggaagacca gtcccgcgcc gtagcgtccg ggctcgggca gcgcgatgcc
     2341 gagggccgcg cactcgcggg tgaggaacag gtgcggcatc tggatgagga tgccggcccc
     2401 gtccccggtg ttgacctcgc agccgcaggc gccccggtgc aggaggttct tcagcaccgt
     2461 gagggcctgg gagacgatgc cgtgggactt gcggcccttg atgtccacca cgaagccgac
     2521 gccacaggcg tcgtgctcgt gggccggatc gtagagtccc tgcgccgggg gcagacccgg
     2581 agccatagcc gacacgtctt cgtgcatcgt tccctctctc caccccggca gtcgcggccg
     2641 gggcccccga agcgttcatc caaccaccgc caaacttccc gagagcctac acgggacgaa
     2701 agcattagcc aagcacaaag taaaaaaaca cgtcattaga gatgctaata gtgctaggtt
     2761 gaccgcgtga acctcgagac cctcaagctc tactgcgacg tggtgaggct ccgcagcttc
     2821 tcccgcggcg ccgccgccaa cgcggtctcc cagtcggcgg cgagccaggc catccagcag
     2881 ctcgaggcgg aggtggacgc ggtcctgctg gaccgctcgc gccgcccgct cctgcccacc
     2941 gagcaggggc aggccttcta cgacagctgc cgcaccctcc tgcagggatg ggagaaggcg
     3001 cgggcggagc tggccgccgc gcgcgagcgc gtggagggca cggtgcgggt ggccgccatc
     3061 tactcggtcg ggctccacga catgagccgg cacatgcagc cgttcatgtc cgcctacccg
     3121 caggcgcggg tgctgctcga gtgcctgcac ccgcacaagg tggtggaggc ggtgctggag
     3181 gacgaggccg acgtcggcat cctctcctat ccggccgcca cgcgggcgct caccgtgctg
     3241 ccgctccgca tggagcccat ggtgctggtc gcgcatcccg cccaccggct ggcccggcgg
     3301 cggacggtga gcccggagga gctccagggc gagaagttcg tggccttcga ccgcgacctg
     3361 gccattcgca aggccatcga ccgggcgctc aggcagcacg gggtcaaggt cgaggtggtg
     3421 atggagttcg acaacgtcga gaccatcaag caggcgatcg gcatcaacgc gggggtgagc
     3481 atcctgccgc gcccgaccgt cctcaacgag tcggggatgc gcacgctggt ggcggcgccg
     3541 ctcggcctgc cctccctcgt gcggccgatc ggcatcatcc accggcgcgg ccggcgtctc
     3601 accccggtgg tggcgcgctt catcgagcag ctccagaagc cggtcgacgc ggcgccgtga
     3661 taccgtagtc ggcatgcgca gcgctcgcgt ttcccccaag gcctgacgag gagtcgtggc
     3721 atgagcgaga ccccggcgtc cctgatcatc gcggcgagcg agagcgattc gaacctcgcc
     3781 tacgcctgcc gcttcctggc ccccgatccc ttcgtcttcc tccaggtcaa cgggcggaag
     3841 accctgctga tgtccgacct cgaggtcgat cgcgcgcggg cccaggcgcg ggtcgacgag
     3901 gtgctctcg
//
LOCUS       DATMCV010000907         5513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_115955, whole genome shotgun sequence.
ACCESSION   DATMCV010000907 DATMCV010000000
VERSION     DATMCV010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115955"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VNU03_20725"
     CDS             complement(<1..726)
                     /locus_tag="VNU03_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWP76957.1"
                     /translation="MTDLHFRSAKQLASDIRRRKVGCLEALDAFLARVDRFNPKLNAI
                     VVTDVDGARRRAKQADAALKKGKVWGPLHGVPMTIKESYDVAGLPTTWGLPALKDNIA
                     PRNALAVDRLLAAGVVLFGKTNVPAWLADYQSYNDVYGTTNNPWDLTRSPGGSSGGSA
                     AALAAGLTGLEAGSDIGSSIRNPAHYCGVFGHKPTFGIVPPRGQALPGRVAQGDISVI
                     GPMARSAEDLAIGLTAMAGADEID"
     gene            complement(754..1455)
                     /locus_tag="VNU03_20730"
     CDS             complement(754..1455)
                     /locus_tag="VNU03_20730"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWP76958.1"
                     /translation="MAVKALLLTLALALSACAPMAGPDGIPRRLSTVTVSGAGTAAAP
                     PDMAEITTGVVTRAATAAQALAQNSQAMERLLQSLGALGIAARDIQTTNVSVSPQRRP
                     AKEGQPADILAYEVVNQVHVKVRDLPSLGRVLDQQVSQGANSIYGIRFGLIDPTPLLD
                     EARKRAMADARRKAELYAAAAGLKVGRVVSVQEAGAASPRPEMGARAMMSAVPVAAGE
                     QEIQASISVTYALEP"
     gene            complement(1455..1949)
                     /locus_tag="VNU03_20735"
     CDS             complement(1455..1949)
                     /locus_tag="VNU03_20735"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWP76959.1"
                     /translation="MEIAGKEGYRHILASSVVFESLPTAALDHISTRGLLVEMESGEP
                     VLAEGTRGPGLYVVLEGLVEIFLPDGAGARIRRPTEVHLNTLGPGRCLGEYSLIDEHS
                     TSAAARSMTEAKLFFLSREEFRGIAEGDARVGKVIYLNLLRFLISRLRAKDEELDLFM
                     MGSL"
     gene            2036..2947
                     /locus_tag="VNU03_20740"
     CDS             2036..2947
                     /locus_tag="VNU03_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWP76960.1"
                     /translation="MSESAPAAGRTTVTLGTATPGGGFPVYGQAVADTINEVDATLHV
                     RPRNTKGSTENVPLLEAGSLDLALVQGEVVHEALTGVGRPPADLRVLAAMYSTPGMFV
                     VRADAPYRSIDDLKGHPVAFGARGSGLIILARYVLDGLGLDRDRDFQAVLLDAAGDGP
                     AMVMDGRAAALWGGGIGWPGFTAVARGPAGGRFLVPDLDGIQRIQRKHPFLKLMLVPA
                     GSYPGQSTELHSVGSWSFIMARPTLADEVAYRLARALHRGEAALGARLAQARETTAAN
                     TVAAVARPEVLHAGVRRYLVEAGLLAR"
     gene            3059..5245
                     /gene="glgX"
                     /locus_tag="VNU03_20745"
     CDS             3059..5245
                     /gene="glgX"
                     /locus_tag="VNU03_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWP76961.1"
                     /translation="MADSKTRIREGLPHPLGATWDGLGVNFALFSAYATKVELCLFDA
                     TGRREIERIELPEYTDEVWHGYLRDLPAGAIYGYRVHGPYEPDTGHRFNPNKLLLDPY
                     AKGHLGKLEWRPEIFGYTIGHQDEDLSFDERDSAPFVPKCRVIDPAFTWTRDRRPGTP
                     WERTVIYEAHVKGLTKRHPLVPEALRGTYAGLAWWDVLEHLKDLGVTAIELLPIHAFV
                     DDGYLLDKKLTNYWGYNTIGFFAPEPRYFANGDASEFKQMVAHLHDAGLELILDVVYN
                     HTAEGNERGPTLSFKGIDNSSYYRLVPDARRHYINDTGTGNTVNLSHPRVLQMVTDSL
                     RYWVAEMHVDGFRFDLATILGREPYGFDQGGGFLDSCRQDPVLNQVKLIAEPWDLGPG
                     GYQVGAFPPGWAEWNDRFRDTVRAYWRGDDGKVGDLASRLAGSSDLFNERGRKPWASV
                     NFVTAHDGFTLNDLVSYNDKHNEANGEENRDGSSNNLSFNHGAEGPTNDAAIRALRER
                     QKRNMLATLILARGTPMMLAGDEFGHTQRGNNNAYCQDNEISWLEWPEIGDEGRALTA
                     FVRKCTFLRHAFPILRRGRFLTAQWNEELQVKDVTWINADGSEMGRPQWDDPHMRCFG
                     MLLDGRGQESGIKRQAGDASLLLVMNAYHDVVKFTLPALVGGSRWLCMLDTNQPERSD
                     SPSFDVGQTYDVTARSFLLFAGLTTGNPGRAVQRIALEFAARSSRD"
     gene            complement(5255..>5513)
                     /locus_tag="VNU03_20750"
     CDS             complement(5255..>5513)
                     /locus_tag="VNU03_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWP76962.1"
                     /translation="APEASVFGDEYIVEKIETKAGWTAPQPDEEWTTGYPQEIQDFVE
                     CVALGREPLSNGPLARDVTAVIYGAYLSAHTGRRIDLRPFL"
BASE COUNT          817 a         1986 c         1936 g          774 t
ORIGIN      
        1 gtcgatctcg tcggcccccg ccatcgcggt gagaccgatc gcgaggtcct ccgcgctccg
       61 cgccatcggg ccgatcaccg agatgtcgcc ctgggcgacg cggccgggca gcgcctggcc
      121 gcgcgggggc acgatcccga aggtcggctt gtggccgaac accccgcagt agtgcgcggg
      181 attgcggatc gacgagccga tgtcgctgcc cgcctcgagg ccggtgagcc cggcggcgag
      241 cgcggcggcc gacccgcccg aggagccgcc cggagagcgc gtcaggtccc atggattatt
      301 ggtggtgccg tagacgtcgt tgtagctctg gtagtcggcc agccacgcgg ggacgttggt
      361 cttgccgaag agcaccaccc ccgccgcgag cagccggtcc accgcgagcg cgttccgcgg
      421 cgcgatgttg tccttcagcg cgggcagccc ccacgtggtc ggcagcccgg ccacgtcgta
      481 cgactccttg atcgtcatcg gcacgccgtg gagcgggccc cagaccttgc ccttcttcag
      541 cgccgcgtcc gcctgcttcg cgcggcgccg cgcgccgtcc acgtcggtga ccacgatcgc
      601 gttgagcttc gggttgaagc gatccacccg cgcgaggaac gcgtcgagcg cctcgaggca
      661 gccgaccttg cggcggcgga tgtcggaggc gagctgcttg gcgcttcgga agtgcagatc
      721 ggtcatggaa tcctcccgga ccttggggcc gcctcagggc tcgagcgcgt aggtcacgct
      781 gatcgacgcc tggatctcct gctcacccgc cgccaccggc accgcggaca tcatcgcgcg
      841 cgcgcccatc tcgggacgcg gcgacgcggc acccgcctcc tgaaccgaca ccacgcgtcc
      901 gaccttgagc ccggccgcgg ccgcgtagag ctccgcctta cgacgggcgt cggccatcgc
      961 ccgcttgcgc gcctcgtcga ggagcggggt cggatcgatc aggccgaacc ggatgccgta
     1021 gatcgagttg gcgccctgcg agacctgctg atcgagcacg cggcccaggc tcgggaggtc
     1081 gcgcaccttc acgtgcacct ggttgaccac ctcgtaggcc aggatgtcgg cgggctggcc
     1141 ctctttcgcc ggccggcgct ggggcgacac gctcacgttc gtggtctgga tgtcgcgcgc
     1201 ggcgatgccg agggcgccca gggactggag cagccgctcc atggcctggc tgttctgggc
     1261 cagggcctgg gccgcggtcg cggcccgggt gacgaccccg gtggtgatct ccgccatgtc
     1321 gggcggcgcg gcggcggtgc ccgcccccga caccgtgacg gtggagagcc ggcgcgggat
     1381 accgtccggg cccgccatcg gcgcgcacgc cgacagcgcg agggcgaggg tgagcagaag
     1441 ggccttcacc gccatcagag tgaccccatc atgaacagat cgagctcctc gtccttggcc
     1501 cgcagccgcg agatgaggaa gcgcagcagg ttgaggtaga tcaccttgcc gacccgggcg
     1561 tcgccctctg cgatgccgcg gaactcctcg cgcgagagga agaagagctt ggcctccgtc
     1621 atggagcgcg ccgccgcgga ggtcgagtgc tcgtcgatca gggagtactc cccgaggcag
     1681 cggccggggc cgagcgtgtt gaggtgcacc tcggtgggcc gccgtatccg cgcgcccgcg
     1741 ccgtcgggga ggaagatctc gacgaggcct tcgagcacca cgtagaggcc gggcccgcgc
     1801 gtaccctccg cgagcaccgg ctcgccggac tccatctcca ccaggagccc gcgggtgctg
     1861 atgtgatcga gcgcggcggt cggcagcgac tcgaacacca ccgagctcgc aagaatgtgt
     1921 cgataccctt ccttgccggc gatctccatc gggggcccat tctagaacag aacgggtcgg
     1981 cgcgcagggg cgcgacgcgg cgcccaccgc ggcgtccgtg tggtaccgta cgcgcgtgag
     2041 cgagagcgcg ccggccgcgg gccgcaccac cgtgacgctc ggcaccgcca ctccgggcgg
     2101 cggcttcccg gtctacggcc aggcggtggc cgacaccatc aacgaggtgg acgcgacgct
     2161 tcacgtgcgc ccgcgcaaca ccaagggctc gaccgagaac gtgccgctgc tggaggcggg
     2221 ctcgctcgac ctggccctgg tgcagggcga ggtggtccac gaggcgctca ccggcgtcgg
     2281 ccgcccgccg gcggacctgc gcgtcctcgc cgcgatgtac tccacccccg ggatgttcgt
     2341 ggtgcgggcc gatgcgccct accggtcgat cgacgatctc aaggggcacc cggtcgcgtt
     2401 cggggcgcgc ggatcggggc tcatcatcct cgcgcgctac gtgctggacg gcctcgggct
     2461 cgaccgcgat cgcgacttcc aggccgtgct cctcgacgcg gcgggggatg ggcccgccat
     2521 ggtcatggac gggcgggccg ccgcgctgtg gggcggcggc atcggctggc cgggcttcac
     2581 cgcggtggcg cgggggccgg cgggcgggcg cttcctggtg ccggatctcg acggcatcca
     2641 gcggatccag cgcaagcacc cgttcctcaa gctgatgctg gtgccggccg gctcctaccc
     2701 cggccagagc accgagctgc actcggtggg ctcgtggagc ttcatcatgg cgcggccgac
     2761 cctcgccgac gaggtggcgt atcgcctcgc ccgcgcgctg caccggggcg aggccgcgct
     2821 cggcgcccgc ctggcccagg cgcgcgagac caccgcggcc aacacggtgg ccgcggtcgc
     2881 aaggccggag gtgctgcacg cgggcgtccg ccggtacctg gtggaggccg ggctcctggc
     2941 ccggtagagg cgacccggta agtccgacgc gcggccgggc gcgactccac ccatccgcgt
     3001 ggagtttcgt ggtggcgtga ccggaatgga acttgctcgg aggcgatcct cgacgcgcat
     3061 ggctgactcg aagacccgga tccgcgaagg cctgccgcac ccgctcggcg ccacctggga
     3121 cgggctcggc gtcaacttcg ccctcttctc cgcgtacgcg accaaggtgg agctctgcct
     3181 cttcgacgcc accggccggc gggagatcga gcggatcgag ctgcccgagt acaccgacga
     3241 ggtgtggcac ggctacctgc gcgacctccc ggcgggggcg atctacggct atcgcgtcca
     3301 cggaccgtac gagccggaca ccggacaccg cttcaacccg aacaagctcc tcctcgatcc
     3361 ctacgccaag ggccacctgg gcaagctcga gtggcggccg gagatcttcg gctacaccat
     3421 cggccaccag gacgaggacc tctccttcga cgagcgggac agcgccccgt tcgtgccgaa
     3481 gtgccgggtg atcgaccccg cgttcacgtg gacgcgcgac cggcggcccg ggacgccgtg
     3541 ggagcgcacc gtcatctacg aggcccacgt caagggcctg accaagcgcc acccgctcgt
     3601 gccggaggcg ctccgcggca cctacgcggg gctcgcctgg tgggacgtcc tcgagcacct
     3661 caaggatctg ggcgtcaccg cgatcgagct gctgccgatc cacgccttcg tcgacgacgg
     3721 ctacctgctc gacaagaagc tcaccaacta ctggggctac aacaccatcg gcttcttcgc
     3781 ccccgagccc cgctacttcg ccaacggcga cgcgtccgag ttcaagcaga tggtcgcgca
     3841 cctgcacgac gcggggctcg agctcatcct cgacgtggtc tacaaccaca ccgcggaagg
     3901 caacgagcgc gggccgacgc tgtcgttcaa gggcatcgat aattcgtcgt actaccgcct
     3961 ggtacccgat gcgagacgcc actacatcaa cgacaccggc accggcaata ccgtgaacct
     4021 gagccacccg cgggtgctcc agatggtgac cgacagcctg cgctactggg tcgccgagat
     4081 gcacgtcgac ggcttccgct tcgacctcgc gaccatcctg ggccgggagc cctacggctt
     4141 cgaccagggc gggggcttcc tcgactcgtg ccggcaggat ccggtgctca accaggtgaa
     4201 gctgatcgcg gagccgtggg acctgggccc gggcggctac caggtcggcg cgttcccgcc
     4261 cggctgggcc gagtggaacg accgcttccg cgacacggtg cgcgcctact ggcggggcga
     4321 cgacggcaag gtgggcgacc tcgcctcgcg gctcgccggc tcgtccgatc tgttcaacga
     4381 gcgcggccgg aagccgtggg cgagcgtgaa cttcgtcacc gcccacgacg gcttcaccct
     4441 caacgacctc gtctcctaca acgacaagca caacgaggcc aacggagagg agaaccgcga
     4501 cggcagctcg aacaacctct cgttcaacca cggggcggag gggccgacga acgacgcggc
     4561 catccgcgcg ctgcgcgagc ggcagaagcg caacatgctg gccaccctga tcctggcgcg
     4621 cggcaccccg atgatgctgg ccggcgacga gttcggccac acccagcgcg gcaacaacaa
     4681 cgcgtactgc caggacaacg agatcagctg gctcgagtgg cccgagatcg gcgacgaggg
     4741 acgcgccctc actgcgttcg tgcgcaagtg cacctttctg cgccacgcct tcccgatcct
     4801 gcggcgcggg cgcttcctca ccgcccagtg gaacgaggag ctgcaggtca aggacgtcac
     4861 gtggatcaac gcggacggct ccgagatggg ccggccgcag tgggacgacc cgcacatgcg
     4921 ctgcttcggc atgctgctgg acgggcgcgg gcaggagagc gggatcaagc gacaggcggg
     4981 ggacgcgagc ctgctgctgg tcatgaacgc gtaccacgac gtggtgaagt tcaccctgcc
     5041 cgcgctcgtc gggggctcgc gctggctctg catgctcgac accaaccagc cggagcgctc
     5101 ggactcgccc tccttcgacg tgggccagac ctacgacgtg accgcgcgct ccttcctgct
     5161 cttcgccggc ctcaccaccg gcaaccccgg gcgcgccgtc cagcgcatcg cgctcgagtt
     5221 cgccgcccgc tcctcgcgcg actgaagggg tcggtcagag gaacgggcgg aggtcgatgc
     5281 ggcggccggt gtgggccgag aggtaggcgc cgtaaatgac cgcggtgacg tcccgggcga
     5341 gcgggccgtt cgagaggggc tcgcggccga gggccacgca ctccacgaag tcctggatct
     5401 cctgcgggta gccggtggtc cactcctcgt cgggctgcgg cgcggtccag cccgccttgg
     5461 tctcgatctt ctccacgatg tactcgtcgc cgaacacgga ggcctccggc gcg
//
LOCUS       DATMCV010000908         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_648030, whole genome shotgun sequence.
ACCESSION   DATMCV010000908 DATMCV010000000
VERSION     DATMCV010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648030"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..774)
                     /locus_tag="VNU03_20755"
     CDS             complement(106..774)
                     /locus_tag="VNU03_20755"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWP76963.1"
                     /translation="MRVIDLSFPIGPHFRWTVASERRSTHEAGQNFQSTVLTVSCHAY
                     THVDAPVHFLPGDRDIASMPVDQWMGEAAVVDLTHLGENAEVTAADLERGAGHVRAGD
                     IVLLRTDWPARCPVESERFWKDAPYTGRGACEWLVARGVKAVGYDYPPDYCIRTGIFS
                     PETKVAREECTTHHVFFPRGITVIEYLTNLDRIGAARCRFIALPLRVTGADGSPVRAI
                     ALVD"
     gene            871..2949
                     /locus_tag="VNU03_20760"
     CDS             871..2949
                     /locus_tag="VNU03_20760"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76964.1"
                     /translation="MAKKPTRWTRREFLRDAALTAGAVGAASLLPPGVAGRAQAQEAK
                     LGAQLIGKLEGPELVLDPAKWPKKFAEAPMLADLVKQGKLPPVDKRVPDEPMVVKPLQ
                     SIGRYGGTWRRGFTGPGDGENGNRIVSTDKILFWDYTGTKIMPCVAKEWKQSDDGKSV
                     TISLRKGQKWSDGTPFTANDFVFWHDEVYLNKDLMPTPHPDFMAGSRSGSLRKIDDTT
                     VIFEFQDPNFLFVDILAGSTAIGGGQATQSMDKRSMGAYMPAHYVKQFLPKFSSKDEV
                     DRKAKAAGFDGWVSYIKNRWDWRLNPELPVLTPWKTVNPINTPTWVLERNPYYMGVDT
                     QGNQLPYIDRISMTLAENLEVLNLRAIAGQYDLQERHTGLAKLPVFLENRAKGGYDVR
                     LDPALNGSDATLQTNQAYDADPEVAKWLHTRDFRRALAMGIDRDQLNETFWVGVGTAG
                     SIAPSETLPYSPGPEWRKKWSTLDVKQANALLDKIGLDKKDGEGYRLRTDGKGRLRLE
                     LQTSAGAFVPHTQIAEMIKEQWKKIGVQADVKEMERSLFFTRIASNEHQIALWANDGS
                     EVLYLFPRHALPLDPVEALLGTPIARWYASNGAQGKAPNDAQMVKALDLFRSAAGKKT
                     QERYKVAQEIWKILVDEQFSIGTVGLSPATMGVRIVSNKLGNIPARQTNAQHARTPCS
                     SHPATFFYKA"
BASE COUNT          519 a         1050 c         1012 g          404 t
ORIGIN      
        1 cggcgaagcc gccgccgacg agaccgagca cggtccacac cgcgacacgc ttgacgagag
       61 cttttgggat atccatggcg agcctttcgt cgggtcgagg ggccgtcagt ccacgagggc
      121 gatcgcgcgc acgggcgagc cgtccgcccc ggtcacgcgg aggggcaggg cgatgaagcg
      181 gcagcgcgcg gcgccgatgc ggtccaggtt cgtcaggtac tcgatcacgg tgatgccccg
      241 gggaaagaac acgtggtgcg tggtgcactc ctcgcgggcg accttggtct ccggcgagaa
      301 gatcccggtg cggatgcagt agtcgggcgg gtagtcgtag cccaccgcct tcaccccgcg
      361 cgccaccagc cactcgcacg cgccgcgccc ggtgtagggg gcgtccttcc agaagcgctc
      421 gctctcgacc gggcagcggg ccggccagtc cgtccgtagc agcacgatgt cacccgcccg
      481 cacgtgcccg gcgccgcgct cgagatccgc cgcggtcacc tccgcgttct cgccgagatg
      541 cgtcaggtcc accaccgcgg cctcgcccat ccactggtcc accggcatgg aggcgatgtc
      601 gcggtccccg ggcaggaagt gcaccggcgc gtccacgtgg gtgtaggcgt ggcagctcac
      661 ggtgaggacg gtcgactgga agttctggcc cgcctcgtgg gtcgagcggc gctcgctggc
      721 caccgtccag cggaagtggg ggccgatcgg gaagctcagg tcgatcacgc gcatggcggt
      781 ccatggtgta cccgggctgc cgcagcttgt caatgttcag ggaatcccgt agactcgcgc
      841 agatccaatc cggcccgagg aggcagcgcc atggcaaaga agcccacgag atggacccgc
      901 cgcgagttcc tgcgtgacgc cgctctcacc gcgggggccg tcggcgccgc ctccctgctg
      961 ccgcccggcg tggccgggcg cgcgcaggcc caggaggcca agctcggggc ccagctcatc
     1021 ggcaagctcg agggcccgga gctggtgctg gacccggcca agtggccgaa gaagttcgcg
     1081 gaggcgccga tgctggcgga cctcgtgaag cagggcaagc tgccgccggt cgacaagcgc
     1141 gtgcccgacg agccgatggt ggtgaagccg ctccagtcca tcggccgcta cggcggcacc
     1201 tggcgccgcg gcttcaccgg gccgggcgac ggcgagaacg gcaaccgcat cgtctcgacc
     1261 gacaagatcc tgttctggga ctacaccggg accaagatca tgccgtgcgt ggcgaaggag
     1321 tggaagcaga gcgacgacgg caagtcggtg acgatctcgc tccgcaaggg ccagaagtgg
     1381 tcggacggca ccccgttcac cgccaacgac ttcgtgttct ggcacgacga ggtgtacctg
     1441 aacaaggacc tcatgccgac cccgcatccg gacttcatgg ccggctcccg gtcggggtcg
     1501 ctgcggaaga tcgacgacac caccgtgatc ttcgagttcc aggacccgaa cttcctcttc
     1561 gtggacatcc tggccggctc caccgcgatc gggggcgggc aggcgacgca gtccatggac
     1621 aagcgcagca tgggcgccta catgcccgcg cactacgtca agcagttcct gcccaagttc
     1681 tcctcgaagg acgaggtgga ccgcaaggcc aaggcggccg gcttcgacgg ctgggtgtcc
     1741 tacatcaaga accgctggga ctggcgcctc aacccggagc tgccggtgct gaccccgtgg
     1801 aagacggtga acccgatcaa caccccgacg tgggtgctcg agcgcaaccc ctactacatg
     1861 ggggtggaca cgcagggcaa ccagctcccg tacatcgacc gcatcagcat gacgctggcc
     1921 gagaatctcg aggtgctcaa cctgcgggcg atcgcgggcc agtacgacct ccaggagcgc
     1981 cacaccgggc tcgcgaagct gccggtcttc ctcgagaacc gggccaaggg cggctacgac
     2041 gtgcgcctcg acccggcgct gaacggctcg gacgccacgc tgcagaccaa ccaggcctac
     2101 gacgccgatc ccgaggtcgc caagtggctg cacacgcggg acttccgccg cgcgctcgcg
     2161 atggggatcg atcgcgacca gctcaacgag accttctggg ttggcgtcgg caccgccggc
     2221 tccatcgccc cctccgagac gctgccctac agccccggcc cggagtggcg caagaagtgg
     2281 tccaccctcg acgtcaagca ggccaacgcg ctcctcgaca agatcgggct cgacaagaag
     2341 gacggcgagg gctaccgcct gcgcaccgac ggcaaggggc gcctccgcct cgagctgcag
     2401 acctccgcgg gggccttcgt gccccacacc cagatcgcgg agatgatcaa ggagcagtgg
     2461 aagaagatcg gcgtccaggc cgacgtgaag gagatggagc gcagcctctt cttcacccgc
     2521 atcgcgtcca acgagcacca gatcgcgctg tgggccaacg acggctcgga ggtgctctac
     2581 ctcttcccgc gccacgcgct gccgctcgat ccggtggagg cgctgctcgg caccccgatc
     2641 gcgcgctggt acgcgagcaa cggggcccag ggcaaggccc ccaacgacgc gcagatggtg
     2701 aaggccctcg atctgttccg ctccgcggcg ggcaagaaga cgcaggagcg ctacaaggtc
     2761 gcgcaggaga tctggaagat cctggtcgac gagcagttca gcatcggcac cgtcgggctc
     2821 tcgcccgcca ccatgggcgt gcgcatcgtg tcgaacaagc tgggcaacat cccggcgcgc
     2881 cagacgaacg cgcagcacgc gcggacgccg tgctcgtcgc acccggcgac cttcttctac
     2941 aaggcgtagc gccccccgtg gcgaggcccc ctcaccctga ccctc
//
LOCUS       DATMCV010000909         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_920533, whole genome shotgun sequence.
ACCESSION   DATMCV010000909 DATMCV010000000
VERSION     DATMCV010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920533"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VNU03_20765"
     CDS             complement(<1..208)
                     /locus_tag="VNU03_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP76965.1"
                     /translation="MEGVSKRYGDHWAVRDVSLSIRPGEFYTLLGPSGCGKTTLLRLL
                     AGFVTPDEGRIVVDDEVIDAVPPWK"
     gene            complement(244..1419)
                     /locus_tag="VNU03_20770"
     CDS             complement(244..1419)
                     /locus_tag="VNU03_20770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP76966.1"
                     /translation="MAPRTRAEWVLPASMLIGTFAWSFIYVSLPFHIQKMSTLDPAST
                     LRWTGWILGISPLITVITAPISGRLGEHIDPKRGFIAVQAFQGLGFLGMALARTLPQM
                     LFARMLLGLMGAVSTFAFIMVGRSGGDVRRQVSYIQSGMTLGQVLGPAAGALVAARVG
                     FQLSFILGAFMLWGCSALVSWGVPPGKPRDAAAAGGRPTSLRELVTVSLVVLAGSTQI
                     FFLTSILPQILPPLGVAPESTLEVGGFLIFATGIAASLGSMAAPRLADLMGDRRAVLW
                     FLVGSSVFLAALSVAGHVWSFGVLRFLQVLCIAPVFPLSVAAIAQRASGEAIGFVNSA
                     RIGAAFLGPVVATTLLSSMPPAAVYLVLAALGLAVVPLVARMDRRARLRTPGGGETS"
     gene            complement(1425..>2178)
                     /locus_tag="VNU03_20775"
     CDS             complement(1425..>2178)
                     /locus_tag="VNU03_20775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP76967.1"
                     /translation="PHAGVLLLSISRVWSLSPLPTVYTLGHYEEILVRSPQFIANTLR
                     YAGLAALIDVALGAVIAWLLVRGRVAGRRWLDILATIPLAIPGVVIAIGFLRAFGGFV
                     VPGLGDPLTSTWLVLVIVYAVRRLPYAVRGAHAALQQLSPALEEAAQNLGASRPRTFR
                     RITLPLMARGLLAGGLLAFISSAVDLSSTILLVPRVELGPLAYGIYLYMQSAVGRGPG
                     AALGVVAIVLVAVGTWAATGLARRSGAALFRS"
BASE COUNT          390 a          787 c          796 g          205 t
ORIGIN      
        1 gcttccacgg cgggaccgcg tcgatgacct cgtcgtcgac cacgatgcgg ccttcgtcgg
       61 gggtgacgaa gccggcgagc aggcgcagca gcgtggtctt cccgcagccg gacggcccca
      121 gcagggtgta gaactcgccc gggcggatcg agagcgagac gtcccgcacc gcccagtggt
      181 cgccgtagcg cttgctcacg ccctccatgc gcacttccga cagccggacc agctgctccg
      241 cgctcacgac gtctcccctc ctcccggcgt ccggagccgc gcccgacggt ccatgcgcgc
      301 cacgagcggc acgacggcca ggccgagcgc ggccagcacc aggtacaccg cggcgggcgg
      361 catcgacgag agcagcgtcg tcgccaccac cgggccgaga aaggcggccc cgatccgcgc
      421 cgagttgacg aatccgatgg cttccccgga cgcccgctgc gcgatggccg ccaccgagag
      481 cgggaacacc ggcgcgatgc agagcacctg caggaagcgc agcaccccga agctccacac
      541 gtggccggcc acggagagcg cggccaggaa caccgaggag ccgaccagga accagaggac
      601 cgcgcggcga tcgcccatga gatcggccag gcgcggcgcc gccatcgacc cgagcgaggc
      661 cgcgatgccg gtggcgaaga tgaggaagcc gcccacctcg agggtggact cgggggcgac
      721 cccgagcgga ggcaggatct ggggcaggat cgaggtgagg aagaagatct gggtggagcc
      781 cgcgagcacc accagcgaca ccgtcaccag ctcccgcagc gaggtggggc gccctcccgc
      841 cgccgccgcg tcgcgcggct tgccgggggg cacgccccac gagacgaggg ccgagcagcc
      901 ccagagcatg aaggcgccca ggatgaacga gagctgaaag cccacccgcg ccgcgaccag
      961 cgcgcccgcg gcgggcccca gcacctggcc gagcgtcatg ccggactgga tgtaggagac
     1021 ctggcgccgc acgtcacccc ccgagcgtcc caccatgatg aacgcgaagg tggagacggc
     1081 gcccatgagc ccgaggagca tgcgggcgaa gagcatctgg ggcagcgtgc gggccagcgc
     1141 catgccgagg aagcccaacc cctggaaggc ctgcaccgcg atgaagcccc gcttgggatc
     1201 gatgtgctcg cccagccggc ccgagatcgg cgcggtgatg acggtgatca gcgggctgat
     1261 tccgaggatc cagccggtcc agcgcagcgt ggacgccggg tcgagggtgg acatcttctg
     1321 gatgtggaag ggcaggctga cgtagatgaa cgaccacgcg aaggtgccga tcagcatcga
     1381 ggccggcaac acccactccg cgcgcgtccg cggcgccatc gcgatcagct ccggaacagc
     1441 gcggcgcccg agcgccgcgc cagcccggtc gcggcccagg tccccaccgc caccagcacg
     1501 atcgcgacca cgccgagcgc cgcgcccgga ccgcgtccca ccgcggactg catgtagaga
     1561 tagatcccgt aggcgagcgg gccgagctcg acccggggca ccagcaggat ggtcgacgag
     1621 agatccaccg ccgagctgat gaacgcgagc aggcccccgg ccagcagccc gcgcgccatc
     1681 aacgggagcg tgatccgcct gaaggtccgc gggcggctcg cccccaggtt ctgggccgcc
     1741 tcctccagcg cgggggacag ctgctggagc gcggcgtggg ccccgcgcac cgcgtagggc
     1801 aggcggcgca ccgcgtacac gatcacgagc acgagccacg tcgaggtgag cgggtcgccg
     1861 agcccgggca ccacgaagcc gccgaacgcg cggaggaagc cgatcgcgat caccaccccg
     1921 gggatggcga gcgggatggt ggcgaggatg tcgagccagc gccggccggc gacccggccg
     1981 cgcaccagga gccacgcgat caccgcgccc agggccacgt cgatcaaggc cgcgaggccc
     2041 gcgtagcgca gggtgttggc gatgaactgc ggggagcgga cgaggatctc ctcgtagtgg
     2101 ccgagcgtgt acacggtcgg caggggcgag aggctccaca cccgcgagat cgacaggagc
     2161 agcacccccg cgtggggc
//
LOCUS       DATMCV010000910         8381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_986353, whole genome shotgun sequence.
ACCESSION   DATMCV010000910 DATMCV010000000
VERSION     DATMCV010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986353"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1040
                     /gene="nuoH"
                     /locus_tag="VNU03_20780"
     CDS             18..1040
                     /gene="nuoH"
                     /locus_tag="VNU03_20780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWP76968.1"
                     /translation="MSALPPLATEAIHAFIVGFIVLNVIIGMVTYVTLLERKFAARMQ
                     SRIGPYRVGPHGLLQPIADALKLMMKEDVVPRQADRRVYNLAPIVFLVPCMLIFATLP
                     FAPGLGIADLNIGILFFLAVSAMEIVGLFMGGWGSNNKYALISAMRAVNQIISYDLPF
                     IFVALIPVVLTGSLRLSDIAAAQAGMPFVLWAPYWVPLGPLAFLAYIVTTLAVENRVP
                     FDILEAESELIAGFRVEYSGMKFALIQLGEYAHVIGTSFLGALLFLGAWSGPWSDTSA
                     GWGATWFLLKAMFVFLLVTWIRWSFVRIRVDQILAISWKLLLPASLVLLMATGLVVAL
                     RSTGNG"
     gene            1033..1572
                     /locus_tag="VNU03_20785"
     CDS             1033..1572
                     /locus_tag="VNU03_20785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWP76969.1"
                     /translation="MARPPLGESFWRDLYELVRAVARAMKVTLINFIRRPVTVHYPTK
                     SRAYPDRYRGLLALTYDKETGEENCIGCRLCEYICPPQVIKVEMLKAEKRNFAKTFTL
                     ELYACEFCELCVQVCPTDAIIMMKSFDMSTADRREMLLDKDRLHDIGLRFEPSWATGN
                     LLRDMQTPPKAAKAGGPAE"
     gene            1569..2063
                     /locus_tag="VNU03_20790"
     CDS             1569..2063
                     /locus_tag="VNU03_20790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWP76970.1"
                     /translation="MSLESIAFWALAVVLLASALAVVLTRNLFHSVLYLALALTATAG
                     VFLSLDAEFLAAIQLLLYAGGVVTIVVFAIVVTERLVGDRITQTSRQIAAGLGIAGAL
                     LVAILAIFRRAPLTVPRPAVSGDLTRAIGQSLLTTYVLPFELLGVLFLAALVGALYFA
                     RPDE"
     gene            2067..2366
                     /gene="nuoK"
                     /locus_tag="VNU03_20795"
     CDS             2067..2366
                     /gene="nuoK"
                     /locus_tag="VNU03_20795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWP76971.1"
                     /translation="MTLQAYLTLAAMLFCIGLFGVLTRRNTIGILLGIELMLNAVNIN
                     LVAFARFLGDVSGMIFTVFTICITVAEVALGLALVILIYRIRRTAVADHLDLLRG"
     gene            2374..4233
                     /gene="nuoL"
                     /locus_tag="VNU03_20800"
     CDS             2374..4233
                     /gene="nuoL"
                     /locus_tag="VNU03_20800"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWP76972.1"
                     /translation="MAASGNFALWAWLAPALSFVLLSLIVPLRRSGKPAAWLSIVLSA
                     GALVAAVSAWRASVPDMARRMLWEWIPVEDGVLTSVGVLADADSTLMLILVALVSFLV
                     QVYSLGYLADEPPASLGRYYTYQSLFAFSMMGLVLAPNFVQLFICWELVGLCSYLLIG
                     YWYTRPEAARAAVKAFWVTKAGDVGFLIGIVMLWSRTGTFDFSELFEMAQGGSDALPG
                     LGLVMFCIYLGAAGKSAQFPFHVWLPDAMEGPTPVSALIHAATMVTAGVYLLFRTAFL
                     FEQTPEVLAAVGWIGAFTALFAATLACVQQDIKRVLAYSTVSQLGYMMAAIGAGFASA
                     GFLHLLTHGVFKALLFLAAGAVIHAVGTNDIFGMGGLARRMPQAFVVFLIGTLSLAGI
                     PFFAGFFSKEEILGATWTGGLTVPFVMLVAAAFLTAFYMFRVVFIAFMSAPAGAAAHG
                     SGHDAAHAHDAPAIMTVPLWILALLAMVIGIGFARSHPEAEFVAPGWLTPLAVGVALG
                     GIVLSAVVYALGWISPAWIFRRLAVLGEAAMERFWLDDFFALLYRRGILELARIVGWV
                     DRYIVDGILNVLSAWTLTAGDRLRRIQSGQAQDYVYGIALGVLLLMVWMRWPR"
     gene            4230..5783
                     /locus_tag="VNU03_20805"
     CDS             4230..5783
                     /locus_tag="VNU03_20805"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWP76973.1"
                     /translation="MSAISLSSFPVLSVITWSPFVGALLIMFLARRSALLVRWLAVVA
                     TAVPLVLSIAIYVAYDREAAGFQFFEDVPLVPALGINYQLGVDGLSLLMVLLTSIIIF
                     AGAWASWTVKVRSQEFYALLLLLVTGVYGVFVSLDLFVLFLFYEIAVLPMYLLIGIWG
                     STGEVRPQGVFGWAFGRTGVGTKEYAAMKLTLYLLFGSAFILVGILALYVAGDSSSFS
                     FLEMELTQFDPKLQSWVFLAFYVGFGILAGIWPLHTWSPDGHASAPTAVSMLHAGVLM
                     KLGAYGVVRLGMGLLPDGTLQWSWLVGTIACVNIVYGALSAMAQTDLKYVIAYSSVSH
                     MGVVMLGAATLTEVGLNGSVYQMFAHGVMTGLFFALVGLVYEKAHSREIFKMGGFGRS
                     MPGIATAFTIAGLSSLGLPATAGFVAEFLTFLGAWQSSHSWWLFPAVIGAYLTAIYVL
                     RVTKQIFWGPPSTDPHFQNLPDARGPEWAALWILVAVILLFGIWPSLALGPVDTATMP
                     LLNRLWVLQ"
     gene            5786..7207
                     /locus_tag="VNU03_20810"
     CDS             5786..7207
                     /locus_tag="VNU03_20810"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWP76974.1"
                     /translation="MPEAGSPLLLEIGLGILMLLVFLIGLFTPGEDRRRVGVLTAVGL
                     VILFGFSWRTEAGAELFHGTFVQDELALFAKRLFLLATLIGVLASLPLRAVTFARRAT
                     EYYLAILASLLGMLVLSSARDLVLLFVAFELMSIPLYYLAGFLKREPEAVEAALKFFL
                     IGTVSSAVLAYGLSFMYGIAGTTDVRGIAHALGAGHPMLLLGMLLALGGLGFKIAAFP
                     FHMWVPDTYEAASTPFVAWLSVAPKAAGFVVIFRLYLEGMGDRVLYWVPAAAALATVT
                     IIGGNLMAIPQQNIKRLLAYSGVAHIGYMLVGFAAVSANGAAMMLFYLVAYLFGNMGA
                     FLVVEVVAQSEGSEAITAYRGLAQRSPILALCMLLFLLSLGGIPFVAGFWAKLYVFWA
                     AAEQGLYWLVLVGALLTVVALFYYLLVAKRMYIDAPERREPVPVPPLLAFCIFLCVLG
                     VVVMGIYPKPLVVAALRAVGPLF"
     gene            7278..>8381
                     /locus_tag="VNU03_20815"
     CDS             7278..>8381
                     /locus_tag="VNU03_20815"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HWP76975.1"
                     /translation="MQGLGIAEGMLLLGCSSPGRQGSTIGSASEAVAPEAVRSVMTRV
                     ADSQLERLAGYPPDDWVVAPFWVGLLTFAPLSRTPARYLESARENGRRNGWQPGPEPF
                     LADDLAITQSYFLLYAVDRDRAEIAPALARFDALLGQRFDESLEFSHDTAEREWVWCD
                     ALFMAPPALALASHAIGDPRYRELMQRLWWKTTDFLYDRETQLYYRDSRFFGQRERNG
                     AKVFWSRGNGWVLAGLARVLQYFPPADPGRRRFEALFRAMAAKVAALQRPDGYWRASL
                     LDPDSWPTPETSGTAFFTYALAWGVNEGLLERRRYDAAVRKGWAALVRAVQPDGRLGY
                     VQRPDDRPAGAEPDHTEPYGTGAFLLAGSEVYRL"
BASE COUNT          986 a         3019 c         2777 g         1599 t
ORIGIN      
        1 cgaggtggac cgctagcatg tccgcgctgc ccccgctcgc cacggaggcg atccacgcct
       61 tcatcgtggg attcatcgtc ctcaacgtca tcattggcat ggtgacctac gtcaccctgc
      121 tcgagcgcaa gttcgcggcg cggatgcagt cgcgcatcgg gccctaccgg gtgggcccgc
      181 acggcctgct ccagcccatc gcggacgcgc tcaagctgat gatgaaggag gacgtggtcc
      241 cgcggcaggc cgatcgccgg gtctacaacc tggccccgat cgtgttcctg gtgccctgca
      301 tgctgatctt cgccaccctg ccgttcgccc ccgggctcgg gatcgccgac ctcaacatcg
      361 gcatcctctt cttcctcgcg gtctccgcga tggagatcgt cggcctgttc atgggcggct
      421 ggggctcgaa caacaagtac gccctgatct ccgcgatgcg cgcggtcaac cagatcatct
      481 cctacgacct gccgttcatc ttcgtggccc tgatcccggt ggtgctgacc ggctcgctcc
      541 ggctctccga catcgcggcg gcccaggccg ggatgccgtt cgtgctctgg gccccgtact
      601 gggttccgct gggcccgctc gcgttcctcg cctacatcgt gaccaccctg gccgtcgaga
      661 accgggtgcc gttcgacatc ctggaggccg agtccgagct gatcgcgggc ttccgcgtcg
      721 agtactcggg catgaagttc gccctgatcc agctcggcga gtacgcccac gtgatcggga
      781 cctcgttcct gggcgcgctg ctgttcctcg gcgcgtggtc ggggccgtgg tcggacacgt
      841 cggccggatg gggcgccacc tggttcctgc tcaaggccat gttcgtcttc ctcctggtga
      901 cctggatccg ctggagcttc gtgcgcatcc gggtggacca gatcctcgcc atctcctgga
      961 agctcctcct gcccgcgagc ctggtgctcc tgatggcgac cggcctggtg gtggccctgc
     1021 ggagcaccgg caatggctag acccccgctc ggcgagagct tctggcgcga cctctacgag
     1081 ctggtccgcg cggtggcccg cgcgatgaag gtcaccctga tcaacttcat ccggcggccg
     1141 gtgaccgtgc actacccgac caagagccgg gcctacccgg atcgctaccg ggggctgctc
     1201 gcgctcacct acgacaagga gaccggcgag gagaactgca tcggctgccg gctctgcgag
     1261 tacatctgcc cgccccaggt catcaaggtg gagatgctca aggcggagaa gcgcaacttc
     1321 gccaagacct tcaccctcga gctctacgcg tgcgagttct gcgagctgtg cgtgcaggtc
     1381 tgcccgaccg acgccatcat catgatgaag tcgttcgaca tgtcgaccgc ggaccggcgc
     1441 gagatgctgc tcgacaagga ccggctgcac gacatcgggc tccggttcga gccctcgtgg
     1501 gccaccggga acctgctgcg cgacatgcag acgccgccga aggcggcgaa ggcgggaggc
     1561 cccgccgagt gagcctcgag tcgatcgcct tctgggccct cgcggtggtg ctgctggcgt
     1621 cggccctcgc ggtggtgctg acccggaacc tcttccactc ggtgctctac ctggcgctcg
     1681 cgctcaccgc gaccgcgggc gtcttcctgt cgctcgacgc ggagttcctc gccgccatcc
     1741 agctcctgct ctacgcgggc ggggtggtga cgatcgtggt cttcgcgatc gtggtcaccg
     1801 agcggctggt gggcgaccgc atcacccaga ccagccggca gatcgcggcc ggcctcggca
     1861 tcgcgggcgc cctgctggtg gcgatcctgg caatcttccg ccgggccccg ttgaccgtcc
     1921 cgcggcccgc ggtctcgggc gacctcaccc gcgcgatcgg gcagagcctg ctcaccacct
     1981 acgtgctgcc cttcgagctg ctcggcgtcc tgttcctggc cgccctggtg ggcgcgctct
     2041 acttcgcgcg gccggacgag tagcccgtga cgctccaggc ctatctcacg ctggccgcca
     2101 tgctcttctg catcgggctg ttcggcgtgc tcacccggcg gaacaccatc ggcatcctgc
     2161 tcggcatcga gctgatgctc aacgcggtca acatcaacct ggtggccttc gcccgcttcc
     2221 tgggcgacgt ctccgggatg atcttcaccg tgttcaccat ctgcatcacg gtggcggagg
     2281 tggcgctggg cctggccctc gtcatcctca tctaccggat ccgccgcacc gcggtggcgg
     2341 accacctgga cctcctcagg ggctagcctc tccatggccg catccggcaa tttcgcgctc
     2401 tgggcctggc tcgcgcccgc cctctcgttc gtgctgctga gcctgatcgt gccgctgcgc
     2461 cgctcgggca agcccgccgc gtggctctcc atcgtgctct cggcgggcgc gctggtcgcc
     2521 gcggtctccg cctggcgcgc gtcggtgccg gacatggcgc ggcgcatgct ctgggagtgg
     2581 atcccggtgg aggacggggt gctgacctcg gtgggtgtgc tcgccgacgc cgactccacc
     2641 ctgatgctga tcctggtggc cctcgtctcc ttcctggtgc aggtctactc gctcggctac
     2701 ctcgccgacg agccgcccgc ctcgctcggc cggtactaca cctaccagtc cctcttcgcg
     2761 ttctcgatga tggggctggt gctggccccc aacttcgtcc agctcttcat ctgctgggag
     2821 ctggtgggcc tctgctccta cctgctgatc ggctactggt acacccgccc cgaggcggca
     2881 cgcgcggcgg tgaaggcgtt ctgggtcacc aaggcgggcg acgtcgggtt cctgatcggc
     2941 atcgtcatgc tgtggagccg caccgggacg ttcgacttct ccgagctgtt cgagatggcc
     3001 cagggcggca gcgacgcgct ccccggcctg ggcctcgtga tgttctgcat ctacctgggc
     3061 gcggccggca agtcggccca gttcccgttc cacgtctggc tgcccgacgc gatggagggc
     3121 ccgactccgg tctcggcctt gatccacgcc gccaccatgg tgaccgcggg cgtctacctg
     3181 ctcttccgca ccgcgttcct cttcgagcag acgcccgagg tgctggccgc ggtgggctgg
     3241 atcggggcct tcaccgcgct gttcgcggcc accctcgcct gcgtgcagca agacatcaag
     3301 cgggtgctgg cctactccac cgtctcccag ctgggctaca tgatggcggc gatcggcgcc
     3361 gggttcgcga gcgcgggctt cctgcacctg ctgacccacg gcgtgttcaa ggcgctgctc
     3421 ttcctcgccg cgggggcggt catccacgcg gtcggcacca acgacatctt cgggatgggc
     3481 ggcctcgccc ggcggatgcc ccaggcgttc gtggtcttcc tgatcggcac cctctcgctg
     3541 gccggcattc cgttcttcgc cggcttcttc tccaaggagg agatcctggg cgcgacgtgg
     3601 acgggcgggc tcaccgtgcc gttcgtgatg ctggtggcgg ccgcgttcct caccgcgttc
     3661 tacatgttcc gcgtggtgtt catcgcgttc atgagcgcgc cggccggcgc cgcggcccac
     3721 gggagcggcc acgacgcggc ccacgcccac gacgcacccg ccatcatgac ggtgccgctc
     3781 tggatcctcg cgctgctggc catggtcatc ggcatcggct tcgcgcgctc gcaccccgag
     3841 gcggagttcg tggccccggg ctggctcacc cccctcgcgg tgggagtggc gctgggcggc
     3901 atcgtgctgt ctgcggtcgt ctacgcgctc ggctggatca gccccgcctg gatcttccgg
     3961 cgcctggccg tgctgggcga ggccgcgatg gagcgcttct ggctcgacga cttcttcgcg
     4021 ctgctctacc gccgcggcat cctcgagctg gcccgcatcg tgggctgggt ggaccgctac
     4081 atcgtcgacg gcatcctgaa cgtgctgagc gcgtggaccc tcaccgcggg cgaccggctg
     4141 cggcggatcc agtcgggcca ggcccaggac tacgtctacg gcatcgcgct cggcgtcctc
     4201 ctcctgatgg tctggatgcg gtggccccgg tgagcgcgat cagcctgtcc tcgttcccgg
     4261 tcctctcggt catcacctgg tcgcccttcg tgggcgccct gctgatcatg ttcctcgccc
     4321 ggcgcagcgc gctgctcgtg cggtggctgg cggtggtggc gaccgcggtg ccgctcgtgc
     4381 tctcgatcgc gatctacgtg gcctacgacc gcgaggcggc gggattccag ttcttcgagg
     4441 acgtgccgct ggtgcccgcc ctcggcatca actaccagct cggggtcgac gggctcagcc
     4501 tgctgatggt cctgctcacg tcgatcatca tcttcgcggg cgcgtgggcc tcgtggacgg
     4561 tgaaggtgcg cagccaggag ttctacgcgc tcctgctcct gctggtcacc ggggtctacg
     4621 gggtcttcgt ctccctcgat ctcttcgtgc tcttcctgtt ctacgagatc gcggtgctgc
     4681 cgatgtacct cctgatcggc atctggggct ccaccggcga ggtgcgcccg cagggcgtct
     4741 tcggctgggc cttcgggcgc accggggtcg gcaccaagga gtacgcggcg atgaagctga
     4801 ccctgtacct gctcttcggg tccgccttca tcctggtcgg catcctggcc ctctacgtgg
     4861 cgggggactc gtcgtccttc tcgttcctcg agatggagct gacccagttc gacccgaagc
     4921 tccagtcgtg ggtcttcctc gccttctacg tgggcttcgg tatcctggcc ggcatctggc
     4981 cgctgcacac ctggtcgccc gacggccacg cctccgcgcc caccgcggtg tccatgctcc
     5041 acgcgggcgt gctcatgaag ctcggggcct acggggtggt gcgcctcggc atggggctgc
     5101 tgccggacgg cacgctccag tggtcgtggc tggtggggac gatcgcgtgc gtgaacatcg
     5161 tctacggcgc gctgagcgcg atggcccaga ccgacctcaa gtacgtgatc gcctactcct
     5221 cggtctcgca catgggcgtg gtcatgctgg gcgcggccac cctgaccgag gtcggcctca
     5281 acggctcggt ctaccagatg ttcgcccacg gggtcatgac cggcctcttc ttcgccctcg
     5341 tcggcctcgt ctacgagaag gcccactccc gcgagatctt caagatgggc ggcttcggcc
     5401 gctcgatgcc cggcatcgcc accgcattca cgatcgccgg cctctcgtcg ctggggctgc
     5461 ccgccaccgc gggcttcgtg gccgagttcc tcacgttcct cggcgcctgg cagtcgtcgc
     5521 actcctggtg gctcttcccc gcggtgatcg gcgcctacct caccgcgatc tacgtgctgc
     5581 gcgtcaccaa gcagatcttc tggggcccgc cgtcgaccga cccgcacttc cagaacctgc
     5641 ccgacgcgcg cgggccggag tgggccgcgc tctggatcct ggtcgcggtg atcctgctct
     5701 tcgggatctg gccgtccctg gcgctgggac cggtggacac cgcgaccatg ccgctgctca
     5761 atcggctgtg ggtgctccag tgagcatgcc cgaggcgggc agcccgctgt tgttggagat
     5821 cggcctcggc atcctgatgc tgctggtctt cctcatcggc ctgttcacgc ccggcgagga
     5881 ccggcgccgc gtcggcgtgc tcaccgccgt cggcctcgtg atcctcttcg gattctcctg
     5941 gcgcaccgag gcgggggccg agctcttcca cggcaccttc gtgcaggacg agctggccct
     6001 gttcgccaag cgcctcttcc tcctggccac cctgatcggg gtgctcgcct ccctgccgct
     6061 gcgtgccgtc acgttcgccc ggcgggccac cgagtactac ctggccatcc tggcctcgct
     6121 gctcggcatg ctcgtgctga gctcggcgcg cgacctggtg ctgctcttcg tggccttcga
     6181 gctgatgtcg atcccgctct actacctggc cggcttcctc aagcgggagc cggaggcggt
     6241 ggaggcggcg ctcaagttct tcctgatcgg caccgtctcc tccgcggtgc tcgcctacgg
     6301 gctctcgttc atgtacggca tcgcgggcac caccgacgtg cgcggcatcg cgcacgcgct
     6361 cggagcgggc caccccatgc tgctgctcgg catgctgctg gcgctgggcg ggctcggctt
     6421 caagatcgcc gcgttcccgt tccacatgtg ggtgcccgac acctacgagg cggcgagcac
     6481 cccgttcgtg gcgtggctgt cggtggcccc caaggcggcc ggcttcgtgg tgatcttccg
     6541 cctctatctc gagggcatgg gcgaccgggt gctctactgg gtccccgcgg cggccgcgct
     6601 cgccaccgtc accatcatcg gcggcaacct gatggcgatc ccgcagcaga acatcaagcg
     6661 cctgctcgcc tactcgggcg tggcccacat cggctacatg ctggtgggct tcgcggcggt
     6721 ctccgccaac ggcgccgcca tgatgctctt ttacctggtg gcctacctct tcgggaacat
     6781 gggggccttc ctggtggtcg aggtggtggc gcagtcggag ggctcggagg ccatcaccgc
     6841 ctaccggggc ctcgcccagc gctcgccgat cctggcgctc tgcatgctgc tcttcctgct
     6901 ctcgctgggc ggtatcccgt tcgtggccgg cttctgggcg aagctgtacg tgttctgggc
     6961 cgccgcggag cagggactct actggctggt gctggtgggc gcgctgctga ccgtggtcgc
     7021 gctcttctac tacctgctgg tggccaagcg catgtacatc gacgcgcccg agcggcgcga
     7081 gccggtcccg gtgccgccgc tgctcgcgtt ctgcatcttc ctctgcgtgc tgggggtggt
     7141 ggtcatggga atctatccca agccgctggt cgtcgccgcc ctccgggccg tcggcccgct
     7201 gttctagagg ccacggcctg atatccgacc ctcgggagga ttccggcctg gggcgccgcc
     7261 ggttctgcct cacgatcatg caggggctcg gcatcgccga gggcatgctc ctcctcggct
     7321 gctcctcacc cgggcgccag ggctcgacga tcggatcggc aagcgaggcg gtcgcgcccg
     7381 aggcggttcg gtccgtgatg acgcgcgtgg ccgactcgca gctcgaacgc ctcgccgggt
     7441 atccgcccga cgactgggtc gtcgcgccgt tctgggtcgg actgctgacc ttcgctccgc
     7501 tgtcgaggac gccggcccgt tacctcgagt cggcccgtga gaacggccgg cggaacgggt
     7561 ggcagcccgg gccggagccg tttctcgcgg acgacctcgc gatcacgcag agctacttcc
     7621 tgctctatgc ggtcgatcgg gaccgagccg agatcgcccc ggccctggcg cgcttcgatg
     7681 ccctgctggg ccagcggttc gacgagtcgc tcgagttctc ccacgacacc gcggagcggg
     7741 agtgggtatg gtgcgacgcc ctgttcatgg cgcctcccgc cctcgcgctc gcttcccacg
     7801 cgatcgggga cccacgctac cgcgagctca tgcagcgtct gtggtggaag acgaccgact
     7861 ttctgtacga ccgtgagacg cagctctact accgcgacag ccgcttcttc ggtcagcgcg
     7921 agcgcaacgg ggccaaggtg ttctggagtc ggggaaacgg ctgggtgctc gccggccttg
     7981 cccgtgtcct gcagtatttc cccccggccg acccggggcg ccggcgcttc gaagctctgt
     8041 ttcgggcgat ggcggcgaag gtcgccgccc tccagcgacc ggacggctac tggcgcgcga
     8101 gcttgctcga ccccgacagc tggccgacgc cggaaaccag cggcaccgcg ttcttcacct
     8161 acgcgctcgc gtggggcgtc aacgaaggcc tgctcgagcg acggcgttac gatgcggcgg
     8221 tccgcaaggg ctgggccgcc ctcgttcgcg cggtccagcc tgacggcagg ctcggctacg
     8281 ttcaacgccc ggatgaccgt ccagccggtg cggagccgga ccacacggag ccatatggga
     8341 ccggcgcatt cctgctcgcc ggcagcgagg tctaccgcct g
//
LOCUS       DATMCV010000911         5376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_847184, whole genome shotgun sequence.
ACCESSION   DATMCV010000911 DATMCV010000000
VERSION     DATMCV010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847184"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..288)
                     /locus_tag="VNU03_20820"
     CDS             complement(55..288)
                     /locus_tag="VNU03_20820"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWP76976.1"
                     /translation="MPTKIRINLANEQELRELPGIGAPEAATIVRFRSEHGPITDAAA
                     LARVLGNRPLADGLAARADFAPADSTAPEAPGA"
     gene            506..1258
                     /locus_tag="VNU03_20825"
     CDS             506..1258
                     /locus_tag="VNU03_20825"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWP76977.1"
                     /translation="MRLAGKVAIITGGAHGMGESEALIFAREGATVVVADVDQAAGAQ
                     VAGKITGGGGKARFAKLDVTSEAEWQDVIRSTAGAYGRLDILVNNAGISGSSDPDTTS
                     TQSWDNIMGVNAKGVFLGMKHAVPAMRQSGGGAIVNISSISGFVGQDRLHMAYNASKG
                     AVRIMTKSGAVQYARDGIRVNSVHPGFMPPMRTSKISADPAWRQPFLETVPMKREGRV
                     EEVAHAVLFLASDEASYITGTELVVDGGFLAV"
     gene            1269..2132
                     /locus_tag="VNU03_20830"
     CDS             1269..2132
                     /locus_tag="VNU03_20830"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HWP76978.1"
                     /translation="MSPSIRALLRGPLPVTAPLVLNPLMARMVEAAGFPAGYLGGGST
                     GYQKVALEANLNLTEMCQAALDIGAVSSLPLILDGACGYGDPMHMRRTIGMSEAAGFA
                     GIEIEDQLVPKRAHHHVGIEHMIPAELMAAKVEAAVGARRSPDFLIIARTNAVRASTM
                     DDALRRGEIYRKAGADVLLLSMAHAPEQLRAIAERLGGPLMYLTRRGGLAGLGMTLAD
                     LGGLGYRIVADPSTPLLAAYEAWKTIYADLGDGFGAGRPRRDWDPLEKDMLGVIELEK
                     LLAIERTTVEK"
     gene            2180..2401
                     /locus_tag="VNU03_20835"
     CDS             2180..2401
                     /locus_tag="VNU03_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76979.1"
                     /translation="MPRTPTKSYQCSVVSETVSISLRRRQSLGGNGKLFVRCSELECQ
                     HIDTNEPPCPLTLALFEAEIAERMAQRAE"
     gene            2584..3855
                     /locus_tag="VNU03_20840"
     CDS             2584..3855
                     /locus_tag="VNU03_20840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWP76980.1"
                     /translation="MSFSDFGLHPDLLRAITALGFQAPTPIQRDAIPPGLAGRDVLAC
                     AMTGSGKTAAFLLPILQRLSALPRGTTRALIVAPTRELAAQIDEHRRELSRFTRIDGA
                     AVFGGVGMGPQEHAFRKKVDILVATPGRLLDHMRMPYARLDGLEVLVLDEADRMLDMG
                     FLPDIKRILKQLPARRQNLLFSATMPPPIAVLAREILRDAVALQIERAPAPAVGVTHT
                     VYPVPQELKSALFVELCKQPGTRSVLAFTRTKHRANRLAEYLAKHGVKADRIHGNRSQ
                     AQRTAALAGFKDGRFQVLVATDIAARGIDIDALSHVINFDVPGMSEDYIHRVGRTARA
                     EAVGDAFTLVSPDEEGDLKGIERAVGTRFSRVRLPSFDYTKRPADKLEIPLAERIASI
                     RARKAEDRQRARINTERRAQHGTPHTARAGR"
     gene            complement(3868..4992)
                     /locus_tag="VNU03_20845"
     CDS             complement(3868..4992)
                     /locus_tag="VNU03_20845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP76981.1"
                     /translation="MARRIVIVGGGLMGLSAAFHLRRADPGAEVTVLEREGVGAAASG
                     ASAAGVRVMGRDPAERALALASLARWPDLDRELEAPTGYRRGGGLRLALDDAAWRAAP
                     AWVAEQRADGVPVELVDAAAARGLAPGVAPACRGGVHCAIDGQADAMATVRAFAAAAR
                     RLGAHVEEGAGARGLAVERGRVAAVERDDGQRLACDAAIVAAGAWTAALLAPLGVRLP
                     IETRPLQMLLTEPAPAGLAPVLGAFDHKLSLKQLDDGAYLIGGGWPARVIDHAANRYE
                     LLEESVKASLETARAVYPPLAGRALARGWAGLEAFTPDDLPMIGPIPGIEGLVIAAGF
                     SGHGFALSPMIGDVLARLALGGEAKLGSGLTFRHLSECKT"
     gene            5063..>5376
                     /locus_tag="VNU03_20850"
     CDS             5063..>5376
                     /locus_tag="VNU03_20850"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HWP76982.1"
                     /translation="MRGPIVYEAPRPPAAPGETVVTSTCGHNCGGRCVVNAHVRDGRI
                     VRISTDPRKWTPEVAPLHACVRGFGQLERLNHPDRVVHPLRRVGPRGGGRFERIGWDE
                     AL"
BASE COUNT          764 a         2024 c         1885 g          703 t
ORIGIN      
        1 acggcgcagg agtataccgt gccgcgggag gagcgggcgc gcggctttgc cgggctaggc
       61 tcccggggcc tcgggcgcgg tggagtcggc cggggcgaag tcggcgcggg cggcgaggcc
      121 gtccgcgagc gggcgattgc ccaggacgcg cgccagcgcg gccgcgtccg tgatcggtcc
      181 gtgctcggac cggaaccgga cgatggtcgc ggcctcgggc gcgccgatgc cgggcagctc
      241 ccgcagctcc tgctcgttcg ccagattgat ccggatcttg gtcggcatac cctggcccag
      301 gagcaaggcg cgtgcccgcg tccacgcctc gccgtgtcgg ccgcatcgcg gcttgcgtgc
      361 cgcgaatcgc gcgcggccgc gtcgagccgg gaaggaatcg cccgggagga gttaccggcc
      421 tcggcctcgt tgcccgcctc attcccgcga gctatagtag gcgccgctcg ttcccagccg
      481 ctctcgtcct ccaggaggaa ccatcatgag actcgcagga aaggtcgcga tcatcaccgg
      541 cggtgcgcac ggcatgggag agagcgaggc gctcatcttc gcccgcgagg gagccaccgt
      601 ggtggtggcc gacgtggacc aggcggcggg cgcgcaggtg gcgggcaaga tcaccggcgg
      661 cgggggcaag gcgcgcttcg ccaagctcga cgtcaccagc gaggcggagt ggcaggacgt
      721 gatccgatcc accgccggcg cctacggccg gctcgacatc ctggtgaaca acgcgggaat
      781 cagcggctcc tcggaccccg acaccacgag cacccagtcg tgggacaaca tcatgggggt
      841 caacgccaag ggcgtcttcc tgggcatgaa gcacgcggtc cccgcgatgc gccagtccgg
      901 gggcggggcc atcgtcaaca tctcgtcgat ctcgggcttc gtgggccagg accggctgca
      961 catggcctac aacgcctcca agggcgcggt gcgcatcatg accaagtcgg gcgcggtcca
     1021 gtacgcgcgc gacggcatcc gggtgaactc ggtgcacccc ggcttcatgc ccccgatgcg
     1081 cacgtcgaag atctcggcgg acccggcgtg gcgccagccc ttcctggaga cggtgccgat
     1141 gaagcgcgag ggccgcgtgg aggaggtggc ccacgcggtg ctcttcctcg cctccgacga
     1201 ggcctcctac atcaccggca ccgagctggt ggtcgacggc ggcttcctcg cggtgtgagg
     1261 cggcggccat gagcccctcc atccgcgcgc tgctgcgggg gccgctgccg gtcaccgctc
     1321 cccttgtcct caacccgctg atggcccgga tggtggaggc ggccggcttc ccggccggtt
     1381 acctgggcgg cggctccacc ggctaccaga aggtggcgct cgaggccaac ctcaacctga
     1441 ccgagatgtg ccaggccgcg ctcgacatcg gcgcggtgtc gtccctgccc ctcatcctcg
     1501 acggggcgtg cggctacggc gacccgatgc acatgcggcg caccatcggc atgagcgagg
     1561 cggccggctt cgcgggcatc gagatcgagg accagctcgt gccgaagcgg gcgcaccacc
     1621 acgtggggat cgagcacatg atcccggccg agctgatggc ggccaaggtg gaggccgcgg
     1681 tgggggcgcg ccggagcccc gacttcctga tcatcgcgcg caccaacgcg gtgcgggcga
     1741 gcaccatgga cgacgccctg cggcgcgggg agatctaccg gaaggcgggc gccgacgtgc
     1801 tgctgctctc gatggcccac gctcccgagc agctgcgcgc gatcgcggag cgcctcggcg
     1861 ggccgctcat gtacctcacc cgccgcggcg ggctggccgg gctcggcatg acgctcgccg
     1921 acctaggcgg gctcggctac cggatcgtgg ccgatccctc caccccgctg ctcgccgcgt
     1981 acgaggcgtg gaagacgatc tacgcggacc tgggcgacgg cttcggggcg ggccggcccc
     2041 ggcgcgactg ggacccgctg gagaaggaca tgctcggcgt gatcgagctg gaaaagctcc
     2101 tcgccatcga gcgcaccacg gtggaaaaat aacgtatact ggtgccctcg gtcgcaatgg
     2161 ccgggagaaa aggaacaccg tgccccggac tcccacgaag tcgtaccagt gcagcgtcgt
     2221 ctccgagacc gtctccatct cgctccgccg ccgtcagtca ctgggcggca acggcaagct
     2281 cttcgtccgc tgcagcgagc tcgagtgcca gcacatcgac accaacgagc cgccctgccc
     2341 gctgaccctg gccctcttcg aggccgagat cgcggaacgg atggcccagc gcgccgagtg
     2401 agccgggccc ggccccgcgg aaaggtttcc gtttccgcgg ggagggcgtg tatgctgtaa
     2461 ccaacttacc tgcatctgca gaccgcgttt cccgtccgct tccgcatcgt ggggcgcgtc
     2521 aagatcatcc gcggaagact ggccccgggc ccaattccct gcccgcaaga ggttacctgc
     2581 tccatgtcgt tttctgattt cggacttcac cccgacctgc tccgcgccat cacggcgctg
     2641 ggcttccagg cgcccacccc catccagcgc gacgcgattc cccccgggct ggccggccgc
     2701 gatgtgctcg cgtgcgccat gaccggcagc ggcaagaccg ccgcgttcct gttgccgatc
     2761 ctgcagcgcc tgtccgccct gccccgcggc accacgcgcg ccctgatcgt ggcgccaacc
     2821 cgcgagctgg ccgcgcagat cgacgagcac cgccgtgagc tcagccgctt cacccggatc
     2881 gacggcgcgg cggtgttcgg cggcgtcggc atgggcccgc aggagcacgc cttccgcaag
     2941 aaggtcgaca tcctggtggc gacccccggg cgcctgctcg atcacatgcg gatgccctac
     3001 gcgcgcctgg acggcctcga ggtgctggtc ctcgacgagg cggaccggat gctcgacatg
     3061 ggcttcctgc cggacatcaa gcgcatcctg aagcagctcc ccgcgcgccg gcagaacctg
     3121 ctcttctccg cgaccatgcc gccgccgatc gcggtcctcg cgcgggagat cctgcgcgac
     3181 gcggtggcgc ttcagatcga gcgggcgcct gcacccgcgg tgggcgtcac ccacaccgtg
     3241 tacccggtgc cccaggagct gaagtcggcc ctgttcgtcg agctctgcaa gcagcccggc
     3301 acccggagcg tgctcgcctt cacccgcacc aagcaccggg ccaaccggct cgccgagtac
     3361 ctcgcgaagc acggggtcaa ggccgaccgc atccacggca accgcagcca ggcccagcgc
     3421 accgccgcgc tggccgggtt caaggacggg cgcttccagg tgctggtggc caccgacatc
     3481 gcggcgcggg gcatcgacat cgatgcgctg agccacgtga tcaacttcga cgtgcccggc
     3541 atgtcggagg actacatcca ccgggtgggc cgcaccgccc gggccgaggc ggtgggtgac
     3601 gccttcacgc tggtgtcgcc cgacgaggag ggcgatctga agggcatcga gcgcgcggtc
     3661 ggcacccggt tctcgcgggt gcgcctgccc agcttcgact acaccaagcg cccggccgac
     3721 aagctcgaga tcccgctggc cgagcgcatc gcgtccatcc gcgcccgcaa ggcggaggac
     3781 cgccagcgcg cccgcatcaa caccgagcgc cgcgcccagc acggcactcc ccacaccgcc
     3841 cgcgccggcc gctagttagt tctggggtca ggtcttacat tccgacaaat gtcggaatgt
     3901 aagacctgac cccagcttcg cttcaccgcc gagggcgagg cgggcgagta cgtcgccgat
     3961 catcgggctc agggcgaagc cgtggccgct gaagccggcg gcgatgacga ggccttcgat
     4021 gccggggatc gggccgatca tcggcaggtc gtccggcgtg aaggcttcga ggccggccca
     4081 gccccgggcc agcgcgcggc cggcgagcgg cggatagacc gcccgcgcgg tctcgaggct
     4141 cgccttcacg ctctcctcca gcagctcgta gcgattggcc gcgtggtcga tcacccgcgc
     4201 gggccagccc ccaccgatca ggtacgcgcc gtcgtcgagc tgcttcaggc tcagcttgtg
     4261 gtcgaaggcc ccgagcaccg gcgcgagccc cgcgggcgcg ggctcggtga gcagcatctg
     4321 gagcggccgc gtctcgatcg gcaggcgcac gccgagcggg gcgagcagcg cggcggtcca
     4381 ggctcccgcg gccacgatcg ccgcgtcaca ggccaggcgc tgcccgtcgt cacgctccac
     4441 cgcggccacg cgcccgcgct ctaccgcgag cccgcgggcc cccgcgcctt cctccacgtg
     4501 cgcgccgagc cggcgcgccg ccgccgcgaa cgcgcgcacg gtggccatcg cgtcggcctg
     4561 cccgtcgatc gcgcagtgca ccccgccgcg gcacgccggc gcgacgccgg gggcgagccc
     4621 gcgcgccgcc gccgcgtcca ccagctcgac cgggacgccg tcggcccgct gctcggccac
     4681 ccacgccggc gccgcgcgcc acgccgcgtc gtcgagcgcg agccgcagcc cgcccccgcg
     4741 ccggtagccg gtgggcgcct ccagctcgcg gtcgagatcg ggccagcggg cgaggctcgc
     4801 gagcgccagc gcgcgctcgg ccggatcgcg gcccatcact cgcacgccgg cggcgctggc
     4861 ccccgaggcg gccgcgccca cgccctcgcg ctcgagcacg gtgacctccg cgcccgggtc
     4921 cgcgcggcgg agatggaacg ccgcgctcag ccccatcagc ccgccgccca cgatgacgat
     4981 ccgccgcgcc atgacgccgc agtctagcac cgggcggtct accggggacc cggcgggaga
     5041 tggtagagtc tccggcagac ccatgcgcgg ccccatcgtc tacgaagccc cgcgaccccc
     5101 cgccgctccc ggcgagaccg tggtgaccag cacctgcggc cacaactgcg gcgggcgctg
     5161 cgtggtgaac gcccacgtgc gcgacgggcg catcgtgcgc atcagcaccg atccgcgcaa
     5221 gtggacgccc gaggtcgcgc cgctccacgc ctgcgtgcgc ggcttcggtc agctcgagcg
     5281 gctgaatcac cccgaccgcg tcgtccaccc gctgcggcgc gtgggcccgc gcggtggggg
     5341 acggttcgag cgcatcggct gggacgaggc cctcga
//
LOCUS       DATMCV010000912         2632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_648075, whole genome shotgun sequence.
ACCESSION   DATMCV010000912 DATMCV010000000
VERSION     DATMCV010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648075"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VNU03_20855"
     CDS             complement(<1..463)
                     /locus_tag="VNU03_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWP76983.1"
                     /translation="MADLVNEFSWSRTRDNVFQECRRRYYYQYYGAWGGWDINSDPLV
                     RRLYILKQLATRQMWIGRLVHEAVERCLLALRDGHGLSEASLIEDTVRQMRAEWKGSR
                     AGLYRETPKRPSLFEHEYAVPIKDSEWQALRDHLVRCLRNFHRLPVLTEIKR"
     gene            629..1486
                     /locus_tag="VNU03_20860"
     CDS             629..1486
                     /locus_tag="VNU03_20860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWP76984.1"
                     /translation="MPKSKAAGLTRRQMLKVGGAATFAGYAVGVEKALGQAIKTDTQG
                     IVAGDATVPIGSYNMPVYEARPANATNAPIVMVISEIWGVHEWVKDVTRRFAKDGYYA
                     IAPELFQREGGVGQIADVQAILKIVLAVPRKQVLGDTTAAVDWAKKRPGVRADKVGVT
                     GHCWGGSTVYQVAATNPDIKAAVAWYGAPARPYPDTPNPVTGFDVVKDIKAPFLGLYG
                     EKDTGPTPEDAKKFGDLLKQTNPNSEIVIYPGVGHGFFADYRPSYNAEAAADAWKRCT
                     GLFAKTLKA"
     gene            1623..1817
                     /locus_tag="VNU03_20865"
     CDS             1623..1817
                     /locus_tag="VNU03_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HWP76985.1"
                     /translation="MPFVNIRIYEGWGKDRLNEIAKRVTAAITDVTQLPKEAVWVVFE
                     EVDPPDWYVGGKPGERMAKK"
     gene            1981..>2632
                     /locus_tag="VNU03_20870"
     CDS             1981..>2632
                     /locus_tag="VNU03_20870"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWP76986.1"
                     /translation="MSSTVTLRDARFTQVVGATIEFERVATGCLFTEGPLWHPAGQYL
                     LWSDMPGDHLRRWSAKDGVTTFRKPCNKSNGLTWDRQGRLLACEHASSQVSRTEPDGR
                     IVPLATHYQGKQLNSPNDIVCGADGSIYFSDPPYGRAEFYGVARPQELPFQGVFRVGP
                     DPKSPVLVANDFDRPNGLCFSLDGRQLFVNDTARQHIRVFDVKTDGTLSGGKVWAET"
BASE COUNT          453 a          906 c          902 g          371 t
ORIGIN      
        1 tgcgcttgat ctcggtcagc accggcaggc ggtggaagtt ccgcaggcag cgcaccaggt
       61 ggtcccggag cgcctgccac tcgctgtcct tgatcggcac cgcgtattcg tgctcgaaca
      121 gcgacggccg cttgggggtc tcccggtaga gcccggcgcg tgagcccttc cactccgcgc
      181 gcatctggcg caccgtgtcc tcgatgagcg aggcttcgct cagcccgtgc ccatcgcgca
      241 gggcgaggag acagcgctcg accgcctcgt gcacgagccg gccgatccac atctggcggg
      301 tggcgagctg cttgaggatg tagagtcgac ggacaagggg gtctgagttg atgtcccagc
      361 cgccccaggc gccgtagtac tggtaatagt agcgccggcg acactcctgg aagacgttgt
      421 cgcgagtgcg cgaccaggag aactcgttga cgaggtcggc cacggcctca gcatagcatc
      481 cacggccggg aaccgagacg gcgtgcgagc agtttccgtg cgcgggggcg gtctgggttg
      541 atggtcccag ggggcgtgat agcctcggcc cgcctcggag gagcgcgcgc agagcgtcat
      601 cccaacgatc accacaagga ggcagccaat gccgaagtcg aaagccgcgg gtctcacgcg
      661 acgccagatg ctcaaggtgg gtggcgccgc caccttcgcc ggctatgcgg tcggggtgga
      721 aaaggcgctc ggtcaggcga tcaagaccga cacgcagggc atcgtcgccg gggacgccac
      781 ggtgccgatc ggcagctaca acatgccggt gtacgaggcc cggccggcca atgccaccaa
      841 tgcccccatc gtcatggtca tctcggagat ctggggggtg cacgagtggg tgaaggacgt
      901 cacgcgccgc ttcgccaagg acggctacta cgcgatcgcg cccgagctgt tccagcggga
      961 gggcggcgtg ggtcagatcg cggacgtgca ggcgatcttg aagatcgtcc tggcggtgcc
     1021 ccgcaagcag gtcctgggcg acaccaccgc cgcggtggac tgggcgaaga agcggcccgg
     1081 cgtccgggcg gacaaggtcg gcgtgaccgg ccattgctgg ggcggctcca ccgtgtacca
     1141 ggtggcggcg accaaccccg acatcaaggc cgcggtggcg tggtacggcg cgcccgcccg
     1201 cccctacccg gacacgccga acccggtgac cggcttcgac gtggtgaagg acatcaaggc
     1261 cccgttcctc ggcctgtacg gcgagaagga caccggcccg accccggagg acgcgaagaa
     1321 gttcggcgac ctgctcaagc agaccaaccc caactccgag atcgtgatct accccggtgt
     1381 cggccacggc ttcttcgcgg actaccggcc ctcgtacaac gcggaggccg cggccgacgc
     1441 gtggaagcgc tgcaccggcc tcttcgcgaa gaccctcaag gcgtaggcgg cctcgagagc
     1501 gcggcgcgcg gctgacgcgc cgcgctcttg ccccctcgcg ggctgcgtgc tagtctcccg
     1561 gcactttccg gggggcaatg tagcgcccct cactcacgcc gccggggcca agggagactc
     1621 cgatgccatt cgtgaacatc cgcatatacg agggctgggg caaggaccgc ctgaacgaga
     1681 tcgccaagcg ggtgaccgcc gcgatcaccg acgtcaccca gctgcccaag gaagccgtct
     1741 gggtggtgtt cgaggaggtc gatccccccg actggtacgt gggcggcaag cccggcgagc
     1801 gcatggccaa gaagtgatca ccgccacgcc ggcaccgcag gccacgaacg tggcgcgggc
     1861 gcctctgacc accaaccata cgatcaaggc ggtggggggg cttggggacg cggggcgagg
     1921 cgcagccgaa ggcgagacga gcgtattcaa gaatccggcg ccgctccccc ccaatacaaa
     1981 atgagcagta ccgtaaccct cagagacgcg cgcttcaccc aggtcgtggg cgccacgatc
     2041 gagttcgagc gcgtcgccac gggctgcctg ttcaccgagg ggccgctctg gcatccggcc
     2101 ggccagtatc tcctgtggag cgacatgccc ggcgatcacc tgcgccggtg gtccgccaag
     2161 gacggcgtca ccacgttccg caagccctgc aacaagtcga acggcctcac ctgggatcgc
     2221 cagggccgtc tgctggcctg cgagcacgcc tcgagccagg tctcgcgcac cgagccggac
     2281 ggtcgcatcg tcccgctcgc cacccactac cagggcaagc agctcaacag ccccaacgac
     2341 atcgtctgcg gggcggacgg ctccatctac ttcagcgatc cgccgtacgg ccgcgccgag
     2401 ttctacggtg tcgcgcggcc gcaggagctg ccgttccagg gcgtgttccg ggtcggcccg
     2461 gatccgaaga gcccggtcct ggtggccaac gacttcgacc ggcccaacgg gctctgcttc
     2521 tcgctcgacg gccggcagct tttcgtcaac gacaccgccc gccagcacat ccgggtcttc
     2581 gacgtgaaga ccgacggcac gctctccggc ggcaaggtct gggcggagac ca
//
LOCUS       DATMCV010000913         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_920576, whole genome shotgun sequence.
ACCESSION   DATMCV010000913 DATMCV010000000
VERSION     DATMCV010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920576"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..488
                     /locus_tag="VNU03_20875"
     CDS             72..488
                     /locus_tag="VNU03_20875"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HWP76987.1"
                     /translation="MPEGARDAADRASIRRITSEILAAVNTSDPGRLLAVWADDGVLM
                     PPDRPAVQGRDALKRHFGEVFVERRFRFSFSLGDVHLAGDVAFERLAYAASVWPAGGG
                     QAMDDAGKGLHVYRRQPDGAWKLALDIWNSDGRASA"
     gene            complement(561..1721)
                     /locus_tag="VNU03_20880"
     CDS             complement(561..1721)
                     /locus_tag="VNU03_20880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP76988.1"
                     /translation="MAETADAIVIGAGIMGAATAYHLARRKYGRVVVLERDAVCSGST
                     ALASGGIRHQYANRIGIELTRRSIEVYEGFAAEFGVDPHFRQHGYLILQQTEEERRLY
                     AESAALQRTMGVDTRVLSPAEVRAGWPYLVSDDLASATYSPRDGFADPYLVTTAIAAR
                     ARELGAVIHQQHPVVAIERRGARAFTVRTPTAEWAAPVVIVAAGCWSGQVGRLAGVEI
                     PVFPRRRCKFITAEIGAERIPLETPFIIDHHSGFSIRREGAGIMVGFGRKDESNRSFD
                     TTPDRDLIPGVAELAVRRLPALEDAPVMRVWAGLYEMTPDQMGILSALPGVDGLFVIA
                     GFSGHGFMHGPIAGQLMAELVADGRAHTVDVAPLDIERFRRGESPVEVMTFV"
     gene            complement(1774..2157)
                     /locus_tag="VNU03_20885"
     CDS             complement(1774..2157)
                     /locus_tag="VNU03_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWP76989.1"
                     /translation="MLLPMMTTSDLDAFFEQGWNGHDVDRLMTFMAEECVFDSASGPE
                     ACGGRHVGREAVRRAFARIFEAFPDVRFEDTRHVVAGDRAVSEWRFAGTTPDGRRMEV
                     DGCDLFTFASGKIARKSSFLKTRTA"
BASE COUNT          368 a          926 c          889 g          365 t
ORIGIN      
        1 ctcttcgcgt tcgacacccc gatcccggag ggcgagcgcg ccccgttctg agcgcgcgcg
       61 gaccgacgac gatgcccgag ggcgcccgcg acgccgccga ccgcgcctcg atccgacgca
      121 tcacgtcgga gatcctcgcc gcggtgaaca catccgaccc cggacgattg ctggcggtct
      181 gggccgatga cggcgtgctc atgcccccgg accgacccgc ggtacagggc cgggacgcac
      241 taaagcgtca cttcggagag gtcttcgtgg agcggcggtt caggttctcc ttctcgctcg
      301 gcgacgtcca cctcgccgga gacgtggcgt tcgagcgcct ggcttatgcc gcgtcggtct
      361 ggcccgcggg cggcggccag gccatggatg atgccggcaa gggtctgcac gtctaccggc
      421 gtcaaccgga cggagcgtgg aagctcgccc tcgatatctg gaacagcgac ggccgggcct
      481 ccgcctagac gcccattctg ccgtcagaca cctattctgc cgtcagacgc ccattctgcc
      541 gtcagacgcc cattctgccg tcagacgaac gtcatcacct cgaccgggct ctccccgcgg
      601 cggaagcgct cgatgtcgag cggcgccacg tcgacggtgt gggcccgccc gtcggcgacc
      661 agctcggcca tgagctggcc cgcgatgggc ccgtgcatga agccgtggcc gctgaagccg
      721 gcgatcacga agaggccgtc gacgcccggc agcgcgctca ggatgcccat ctggtcgggc
      781 gtcatctcgt agagcccggc ccacacgcgc atgaccggcg cgtcctccag cgcgggcagc
      841 cgccgcaccg ccagctccgc cacgccgggg atcaggtcgc ggtcgggcgt ggtgtcgaag
      901 ctccggttgc tctcgtcctt gcgcccgaag cccaccatga tgcccgcgcc ctcgcggcgg
      961 atcgagaagc cggagtggtg gtcgatgatg aacggcgtct cgagcgggat gcgctcggcc
     1021 ccgatctccg cggtgatgaa cttgcaccgc cggcgcggga acaccggaat ctccacgccc
     1081 gcgagccggc ccacctgccc cgaccagcag ccggccgcga cgatcaccac cggcgccgcc
     1141 cactcggcgg tcggggtgcg caccgtgaag gcgcgggccc cgcggcgctc gatggccacc
     1201 accgggtgct gctggtggat gaccgcgccc agctcgcgcg cccgggccgc gatggcggtg
     1261 gtcaccaggt agggatcggc gaagccgtcg cgcggcgagt aggtcgcgga ggcgaggtcg
     1321 tcgctcacga ggtacggcca gcccgcgcgc acctcggccg gcgacagcac gcgggtgtcc
     1381 acgcccatcg tgcgctggag ggccgcgctc tcggcgtaga ggcgacgctc ctcctcggtc
     1441 tgctgcagga tgaggtagcc gtgctggcgg aagtggggat ccacgccgaa ctccgcggcg
     1501 aatccctcgt agacctcgat ggagcggcgg gtcagctcga tgccgatgcg attggcgtac
     1561 tggtggcgga tgccgccgct ggccagcgcg gtcgagccgg agcagaccgc gtcgcgctcc
     1621 agcacgacga cccggccgta tttccgccgc gcgaggtggt aggcggtggc ggcgcccatg
     1681 atccccgccc cgatgacgat cgcgtccgcg gtctcggcca tggcgcgcgg atcatagcac
     1741 cggcggggtc gaccccgcgc gccggaccgc gcctcaggcg gtacgggtct tgaggaacga
     1801 gctcttgcgc gcgatctttc cgctcgcgaa ggtgaagaga tcgcagccgt cgacctccat
     1861 ccgccgcccg tcgggggtgg tccccgcgaa gcgccactcc gagaccgcgc gatcgcccgc
     1921 caccacgtgg cgggtgtcct cgaaccgcac gtcggggaac gcctcgaaga tccgcgcgaa
     1981 ggcgcggcgc accgcctcgc gcccgacgtg ccggccgccg caggcctcgg ggccggaggc
     2041 gctgtcgaac acgcactcct cggccatgaa ggtcatcagg cggtcgacgt cgtggccatt
     2101 ccatccctgc tcgaagaacg cgtccagatc gctcgtggtc atcatgggca gcagcatcgc
     2161 gcccgcccgt gcgatcggtc aattccccat acaactggta ggtgcgcgcg cggggcccca
     2221 tgtatggcgt cggtggggtg ggctgccccc atacatctat gcggcggcgg gtaccgctcc
     2281 cacatcttgc gacggcccct caccctgccc tctccccgga ggggagaggg tgaaaggaga
     2341 ggggtggcag ctgtgctagg atcggccact cgaaaggact cctcccatga ccattcgcgt
     2401 cgccggcatc gaggtcggtc actggcactc actcttcgat gccgcgtacc tgcggcatct
     2461 cgtcaagatg cccgacgtgg cgctggtcgc ggtgcaggac agcgacggcg cgctcgtccg
     2521 caagcgcgcg gccgaggtcg gcaacccc
//
LOCUS       DATMCV010000914         6077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_782856, whole genome shotgun sequence.
ACCESSION   DATMCV010000914 DATMCV010000000
VERSION     DATMCV010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782856"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VNU03_20890"
     CDS             <1..200
                     /locus_tag="VNU03_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76990.1"
                     /translation="PAGLITTTVFQRLALGEMAALGATGLPLLVIQHPLGGEAPEGVS
                     RRTVQAVEQLAAMLGGASGPR"
     gene            197..1249
                     /locus_tag="VNU03_20895"
     CDS             197..1249
                     /locus_tag="VNU03_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76991.1"
                     /translation="MSETVDASLTLPEEDVYAEFCKREWCDGLPFVPPTPERVRAMLA
                     GARTKAAESLGVMPPLWRECAVEKLAVNAVMAGCEPAYFPVIVAAVQCMLDPEFNLYG
                     VQATTHPVAPLVVVSGPYARTIGMHTGSGLFGPGFRPNATIGRAIRLILMNVGGGWPG
                     RGDMATQGSPAKFAYAIAEREDSPWEPLHVALGFRADQSVVTVFGGEGPHNVNDHVST
                     TAPGILNNVADVATSLGSNVGWYMSQSQMLVVLGPEHAATIAADGFSRADVQHFVFEH
                     ARIPLGRMKLGGMWSMRDWPEWMNKVTDESVRLPRVPAVSDIYVMVGGGSGKHSCVVP
                     NCTFSRAVSRPLDRIP"
     gene            complement(1254..2150)
                     /locus_tag="VNU03_20900"
     CDS             complement(1254..2150)
                     /locus_tag="VNU03_20900"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWP76992.1"
                     /translation="MLLGLILVAVAAVSWGTTGATMTLLSRDAAASPLLVGWSRLAVA
                     APVLLLVAGLAARVGALRSPRRAWPARADLPLCAALGLAMAAYQVCFFRAVTLIGVAA
                     AALLAICTAPLLIAVLAALTLGERLTLRVQLSLGMAVAGTALLVVGPRGLGEITGHFG
                     RGALLALGAGVSYAVYAVAAKGLLARVAPISVSAVTFGLAALFLAPALLGEDAPARTL
                     AAGWPLFLYLGVGPTAVAYALFTAGLTRVPATAAGIVSLLEPLTATTLGLLVFGERLG
                     AVGAAGALLLLAALALLTTARR"
     gene            2179..3093
                     /locus_tag="VNU03_20905"
     CDS             2179..3093
                     /locus_tag="VNU03_20905"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP76993.1"
                     /translation="METGDTIGFIGLGNMGAPMAGRLLDAGYSLVVHDAREAAAGPLR
                     ARGAAWATTPAEVAAAAQTVITSLPTSREVRQVLTGPKGLLDALRPGSLLLEMTSADP
                     SVTRELAAEVAARGSALIDAPVSGGVRGATEGTLAIMVGGDPALLERARPVLARMGKN
                     IFHAGPVGAGHAIKLVNNMCSGGILALTIEAVAVAARSGVDPARAVEIIQASSGRSNA
                     SDYKFPRFILTGGFDAGFAIRLMMKDLDGYGRLAQEAGVPSPVARAAAEIYRLAMARG
                     MGEQDHTAIARLIEEWAGVSLRTEGEKA"
     gene            3090..3995
                     /gene="mmsB"
                     /locus_tag="VNU03_20910"
     CDS             3090..3995
                     /gene="mmsB"
                     /locus_tag="VNU03_20910"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HWP76994.1"
                     /translation="MTRIGFIGVGTMGLPMAKNLVQKGFAVTAFDSNPEAVKAAAAAG
                     MTAAATAAEAVATADIVVTMLPSSPHVESVYTGDGGVLAAARKGTLCVDMSTIDPAVS
                     QRVAKAAQERGVRFMDAPVSGGTPRATDGTLAIMVGGAATDFEEARPALSAMGANVIH
                     VGPVGSGEVVKLCNNLIAGVCGVAVSEAFRIAEGFGMDPKVVTDVISKSSGNTFLMGF
                     MHPVPGLMPNAASSNDYKPGFMTDLMCKDLGLAVDAARNLRVPLFVTPAAQQVYRLAS
                     SHGLGRRDFTSVYSFLKPSSGQAPV"
     gene            4015..>6077
                     /locus_tag="VNU03_20915"
     CDS             4015..>6077
                     /locus_tag="VNU03_20915"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWP76995.1"
                     /translation="MSRRLRRWLIVLGVLVVLGVAFIWSLPEIVKWQALKQIPAITGR
                     QASIEDIDINLFTGRVAIKKFRLAEQDSAQTFVEFERFDVRVVPWSIVFGNVRVAELR
                     LTAPTVNLVRMSTTEFNFSDILKRLATPKEGAPPPKPVDPKAKSRWTIAVSHFALIRA
                     NITAVDRAVTPTSEWKVRDLSIEAGNLTTRPSGPVGTLAVRAALNDSPVEFSAEDIDL
                     TPTSFAGKFSIQNFSLLQVRPYLPPLPATLDSGIAGVKMAVKLELGPGPGGLAVGLVT
                     GDVGIASLALSQPGKTTPFLSVPRIAVELKEVNLVSRVVTLSSVDIEGLDLRAVRDKQ
                     EQIDLLELTKKPAGAPTPAAPAPAASSKPAPAAKPPAAGGAGAPAPSPPAAPADAGYK
                     ITVEKVTLKGSATFTDESVSSPPTVLKIAGLNLQVSDVTWPAARPMQLDLAMILPGGG
                     KFGAKGPVGIDPVDVTLALTTRDAPIEPYHAYFPFPASFSGRFNAETQNRVRIVNGKL
                     TALSKGNSWATDLAVKEPDGKDAPLKLQRLEINGIDFAWPTYARVARIRLKQPVGEVD
                     RAADGTINVQKLFTPTPKPGAPASPAPPAKAPEKPAPAGPAAKSQGPLETMELFFKEI
                     VIEDGYTRFLDHTTQPAFSEDVSKINLSVKNLSNKASQRASIAMQAVVGGDSALDLRG
                     EMAAIG"
BASE COUNT          915 a         2148 c         2228 g          786 t
ORIGIN      
        1 cccccgcggg actgatcacc accaccgtgt tccagcgcct ggcgctgggc gagatggccg
       61 cgctcggcgc gaccggactg cccctgctcg tgatccagca cccgctgggc ggcgaagcgc
      121 cggagggcgt gagccgccgg acggtgcagg cggtggagca gctcgccgcg atgctcgggg
      181 gggcgtccgg cccccgatga gcgagacggt cgacgccagc ctgaccctgc cggaagaaga
      241 cgtctacgcg gagttctgca agcgggagtg gtgcgacggg ctgccgttcg tcccgccgac
      301 ccccgagcgc gtgcgcgcga tgctggccgg cgcgcgcacc aaggccgccg agtccctcgg
      361 ggtcatgccg ccgctctggc gcgagtgcgc ggtcgagaag ctggccgtga acgcggtgat
      421 ggcgggctgc gagcccgcct atttcccggt catcgtcgcg gcggtgcagt gcatgctcga
      481 tcccgagttc aacctctacg gcgtccaggc caccacgcat ccggtggcgc cgctcgtggt
      541 ggttagcggg ccgtacgcgc ggaccatcgg catgcacacc gggtccgggc tcttcggccc
      601 cgggttccgc cccaacgcca ccatcgggcg cgcgatccgg ctcatcctga tgaacgtggg
      661 cgggggatgg cccgggcgcg gggacatggc cacccagggg agcccggcca agttcgccta
      721 cgcgatcgcc gagcgtgaag attccccgtg ggagccgctg cacgtggcgc tcggtttccg
      781 cgccgaccag agcgtggtca ccgtcttcgg cggcgagggc ccgcacaacg tcaacgacca
      841 cgtctccacc accgcgcccg gcatcctgaa caacgtggcc gacgtggcca ccagcctcgg
      901 ctccaatgtg ggctggtaca tgtcgcagag ccagatgctc gtggtcctcg gccccgagca
      961 cgcggccacc atcgcggcgg atggcttctc gcgcgccgac gtgcagcact tcgtcttcga
     1021 gcacgcgcgc atcccgctcg ggcggatgaa gctgggcggg atgtggagca tgcgcgactg
     1081 gccggagtgg atgaacaagg tgaccgacga gtcggtgcgg ctgccgcgcg tgcccgcggt
     1141 gagcgacatc tacgtgatgg tgggcggcgg ctccggcaag cactcctgcg tcgtccccaa
     1201 ctgcaccttc agccgcgccg tctcccgtcc cctcgaccgc atcccctgaa gagctagcgg
     1261 cgcgcggtgg tgaggagcgc gagggccgcc aggagcagca gggcaccggc ggcgcctacc
     1321 gcgcccaggc gctcgccgaa gacgagcagc ccgagcgtgg tggcggtgag cggctcgagc
     1381 agcgagacga tgccggccgc ggtcgcgggc acgcgggtga gtcccgcggt gaagagcgcg
     1441 taggcgaccg cggtcggtcc cacccccaga tagaggaaga gtggccagcc cgccgcgagc
     1501 gtccgcgcgg gcgcgtcctc gccgagcagg gccggcgcga ggaagagcgc ggccagcccg
     1561 aacgtgaccg cggacaccga gatcggcgcg acgcgggcca ggagcccctt tgcggccacc
     1621 gcgtacacgg cgtaagatac ccccgcgccg agcgccagga gcgcgcctcg cccgaagtgg
     1681 cccgtgatct cgccgagccc gcgcgggccg accacgagca gcgcggtccc ggccaccgcc
     1741 atcccgagcg acagctgcac gcgaagtgtg aggcgctccc cgagagtgag ggcggccagc
     1801 accgcgatga ggagcggggc ggtgcagatg gcgagcagcg cggcggcggc gacgccgatc
     1861 agcgtcaccg cgcgaaagaa gcagacctgg taggcggcca tggccaggcc gagggcggcg
     1921 cagagcggca ggtcggcgcg cgcgggccac gcgcgtcggg gggagcggag cgcgccgaca
     1981 cgggcggcga gcccggcgac cagcaggagc accggggccg cgacggcgag ccggctccac
     2041 ccgacgagga gcgggctcgc cgcggcgtcg cgggagagca gggtcatggt cgcgccggtg
     2101 gtgccccacg acacggcggc aacggcgacg aggatcaggc cgagcagcat gggcgggtag
     2161 catacaggag ggacgcgcgt ggagacgggc gacacgatcg gcttcatcgg gctgggcaac
     2221 atgggtgcgc cgatggcggg ccgcctgctg gacgcgggct attccctggt cgtccacgac
     2281 gcgcgcgagg ccgcggccgg gccgctgcgg gcccgcggcg cggcgtgggc cacgacgccg
     2341 gcggaggtgg cggcggccgc ccagaccgtg atcacgagcc tgccgacctc gcgcgaggtg
     2401 cggcaggtgc tgaccgggcc caagggcctg ctcgacgcgc tgcggcccgg cagtctcctg
     2461 ctcgagatga cgtcggccga tccctcggtc acccgcgagc tggccgcgga ggtggccgcg
     2521 cgcggctcgg ccctcatcga cgcgccggtc tcgggcggcg tgcgcggggc caccgagggc
     2581 acgctcgcga tcatggtggg cggcgatccc gcgctgctcg agcgcgcccg gccggtgctc
     2641 gcccgcatgg gcaagaacat cttccacgcg ggcccggtgg gcgccggcca cgcgatcaag
     2701 ctcgtcaaca acatgtgctc cggcggcatc ctcgcgctca ccatcgaggc ggtggcggtg
     2761 gcggcgcggt ccggggtgga cccggcgcgg gcggtcgaga tcatccaggc ttcgagcggg
     2821 cgctccaacg cgagcgacta caagttcccg cgcttcatcc tgaccggcgg cttcgacgcc
     2881 ggcttcgcga tccggctcat gatgaaagac ctcgacggct acggccgtct cgcccaggag
     2941 gcgggggtgc cgtcgccggt ggcccgcgcc gcggccgaga tctaccggct ggccatggcc
     3001 cgcggcatgg gcgagcagga ccacaccgcg atcgcccgcc tcatcgagga atgggcaggc
     3061 gtctcgctcc gtaccgaagg agaaaaggca tgacgcgcat cggcttcatc ggggtgggca
     3121 ccatggggct gcccatggcg aagaacctcg tccagaaggg cttcgcggtc accgcgttcg
     3181 acagcaaccc ggaggccgtg aaggcggccg ccgcggcggg catgaccgcg gccgcgaccg
     3241 ccgcggaggc ggtggccacc gcggacatcg tggtgaccat gctgccctcg tcgccccacg
     3301 tcgaatcggt gtacacgggg gatggcgggg tcctggccgc cgcccgcaag ggcacgctgt
     3361 gcgtggacat gtccaccatc gaccccgcgg tgtcgcagcg ggtggccaag gcggcgcagg
     3421 agcgcggcgt gcgtttcatg gacgcgccgg tctccggcgg caccccgcgg gccaccgacg
     3481 gcacgctcgc gatcatggtg ggcggcgcgg cgacggactt cgaggaggcc aggcccgcgc
     3541 tctccgcgat gggggccaac gtgatccacg tggggccggt gggcagcggg gaagtggtga
     3601 agctctgcaa caacctgatc gcgggggtgt gcggggtggc ggtgagcgag gccttccgca
     3661 tcgcggaggg cttcgggatg gacccgaagg tggtcaccga cgtcatctcc aagtcctccg
     3721 gcaacacctt cctgatgggc ttcatgcacc cggtcccggg gctcatgccg aacgcggcct
     3781 ccagcaacga ctacaagccg ggcttcatga ccgacctcat gtgcaaggac ctggggctcg
     3841 cggtggacgc ggcccgcaac ctccgggtgc cgctcttcgt gacgcccgcc gcccagcagg
     3901 tctatcggct cgcgtcttcc cacgggctcg gccgccgcga cttcacgagc gtctactcgt
     3961 tcctgaagcc gtcctcgggc caggccccgg tctaggcggg gaggcgcgca cgcgatgagt
     4021 cgccggctcc ggcgctggct gatcgtcctg ggagtgctcg tggtcctggg agtggccttc
     4081 atctggagcc tgcccgagat cgtgaagtgg caggcgctca agcagatccc ggccatcacc
     4141 gggcggcagg ccagcatcga agacatcgac atcaacctct tcaccgggcg ggtggcgatc
     4201 aagaagttcc gcctcgcgga gcaggattcc gcccagacct tcgtcgagtt cgagcgcttc
     4261 gacgtacggg tcgtgccgtg gtcgatcgtc ttcggcaacg tccgggtcgc cgagctccgc
     4321 ctcaccgcgc ccaccgtgaa cctggtgcgc atgagcacga ccgagttcaa cttctcggac
     4381 atcctgaagc ggctcgcgac ccccaaggaa ggggcgccgc cgcccaagcc ggtcgatccc
     4441 aaggccaagt cgcgctggac gatcgcggtg agccacttcg cgctgatccg ggccaacatc
     4501 accgcggtcg accgcgcggt cacccccacc agcgagtgga aggtgcgcga tctgagcatc
     4561 gaggcgggga acctcaccac ccggccgtcc ggcccggtgg ggaccctcgc ggtgcgcgcc
     4621 gcgctgaacg actcgccggt cgagttctcg gccgaggaca tcgacctcac tcccacctcg
     4681 ttcgccggga agttctcgat ccagaacttc agcctgctcc aggtccggcc ctacctgccg
     4741 cccttgcccg ccaccctcga ctccgggatc gcgggcgtga agatggcggt gaagctagag
     4801 cttggcccgg ggccgggcgg cctcgcggtc gggctggtca ccggcgacgt cgggatcgcc
     4861 tcgctcgcgc tcagccagcc gggcaagacc acgccgttcc tctccgtgcc gcgcatcgcg
     4921 gtcgagctca aggaagtgaa cctggtctcg cgcgtggtca ccctgtcgag cgtcgacatc
     4981 gaggggctcg atctcagggc ggtccgcgac aagcaggagc agatcgattt gctggagctc
     5041 accaagaagc cggcgggcgc gccgaccccg gcggcccccg cgccggccgc atcgagcaag
     5101 ccggcccccg ccgccaagcc ccccgccgct gggggcgcgg gtgctcccgc tccctcaccg
     5161 ccggccgcac ctgccgacgc cggttacaag atcacggtgg agaaggtcac cctcaagggg
     5221 tcggccacgt tcaccgacga gtcggtctcg tcgccaccga cggtgctcaa gatcgcgggc
     5281 ctgaaccttc aggtgagcga cgtgacctgg ccggcggcgc ggccgatgca gctcgacctg
     5341 gcgatgatcc tgccgggcgg cggcaagttc ggcgcgaagg ggccggtggg gatcgatccg
     5401 gtcgacgtga ccctggccct gacgacgcgc gacgcgccga tcgagccgta tcatgcctac
     5461 ttcccgttcc cggccagctt ctcggggcgc ttcaacgcgg agacccagaa ccgggtccgg
     5521 atcgtcaacg gcaagctcac cgcgctctcc aagggcaata gctgggcgac cgacctggcg
     5581 gtgaaggagc cggacgggaa ggacgcgccg ctgaagctgc agcgcctcga gatcaacggc
     5641 atcgatttcg cgtggcccac gtacgcgcgg gtggcccgca tccggctcaa gcagccggtg
     5701 ggcgaggtcg atcgcgcggc ggacgggacc atcaacgtgc agaagctctt cacgcccacg
     5761 ccgaaaccgg gcgcgcccgc gagcccggct cctcccgcga aggcgcccga gaagccggcg
     5821 ccggccgggc cggccgcgaa gtcgcaaggg ccgctcgaga cgatggagct cttcttcaag
     5881 gagatcgtga tcgaggacgg ctacacccgc ttcctcgacc acaccaccca gccggccttc
     5941 tcggaggacg tctccaagat caacctgtcg gtcaagaacc tatccaacaa ggcgagccag
     6001 cgcgcgagca tcgcgatgca ggcggtggtg ggcggtgact ccgcgttgga tcttcggggg
     6061 gagatggcgg cgatcgg
//
LOCUS       DATMCV010000915         3042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_920749, whole genome shotgun sequence.
ACCESSION   DATMCV010000915 DATMCV010000000
VERSION     DATMCV010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920749"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VNU03_20920"
     CDS             complement(<1..585)
                     /locus_tag="VNU03_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP76996.1"
                     /translation="MARRTRSFTGLLLAGLLLGSLVAAGPAGAQSGAQPKRGGVIRIA
                     EREAPGLDPHLTISFLSHSYISLSYSQLVRFPNGPEQKSPTDFSILPDLAEKWTVSKD
                     GKAYTFNIRKGVKFHNKPPVNGREVTADDVKFSLERFMAKSGFRDRFEPVTGIEVVDR
                     YTVKITLKEPYAPFLNHLASPSFCAILPREAEEKF"
     gene            781..2058
                     /locus_tag="VNU03_20925"
     CDS             781..2058
                     /locus_tag="VNU03_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase"
                     /protein_id="HWP76997.1"
                     /translation="MALARSRPVMQQIAPDNPKLAVVMVGLPARGKSYTARRIERYLS
                     WLGYRTGVFNVGEYRRARFGAHRHSFFDPDNATGEEARWRAAMDALEDMVRWLAGDGQ
                     VAIYDATNTTRIRRAAVRECCERAGFQVLFIEIRCDDPSIIEANIRSTKLSSPDYEDA
                     DPDEATADFRARIAHYERVYQPLEDDEGAYLRVTGAGRGVTASDVDGYLEARLVFFLM
                     NLHLSARPIWLTRHGESAYNAAGLIGGDPDLTPGGHRYARSLAEFLDQRYPDHGDVVI
                     WTSSLRRAVQTASPLDRGPWAWRALDEIDAGICDGLSYAQIREQLPEEYEARRRDKFS
                     YRYPRGESYQDIILRLDPVIIELERTRRPVMVIAHNAVLRALYAYFQGVPRERCPYLT
                     IPLHTVIELTPHAYGCHESRVPLEPRLDSDEAR"
     gene            complement(2063..>3042)
                     /locus_tag="VNU03_20930"
     CDS             complement(2063..>3042)
                     /locus_tag="VNU03_20930"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP76998.1"
                     /translation="GETLARETSWIVTLTDAEIADVDRAVAAAKASGLPLESIQREHF
                     PLAVLGPRLEQAVAEMYDGRGFLVLRGLPVRRYRDEDVGLIFWGFGRYMGAPLYQNPQ
                     GELLGHVYDHGRTYGNIDVRGYETNAYLPYHTDAGDVVGLLCLRRSLEGGLSSIVSST
                     TVHNEILAQHPEYLGLLYNGYYYIRREAALTERGVSERPIPVFGYQDGLVSCRYIRNQ
                     INAGAVKREVPLTTFERAALDFLDEQTRRPDLRLDMDLEAGDIQFINNYTILHSRTGF
                     VDGPEPDQKRHMLRLWLKFPKRWPLSPEFPTHMGYKPSQDTPELVEAER"
BASE COUNT          451 a         1000 c         1111 g          480 t
ORIGIN      
        1 gaacttctcc tccgcctcgc gtggcaggat cgcacagaag ctcgggctcg ccaggtggtt
       61 caggaacggg gcgtagggct ccttgagcgt gatcttcacg gtgtagcggt cgacgacctc
      121 gatgccggtc accggctcga agcgatcgcg gaagccggat ttggccatga agcgctcgag
      181 ggagaacttg acgtcgtcgg cggtgacctc gcgcccgttc accggcggct tgttgtggaa
      241 cttcacccct ttgcgaatgt tgaacgtgta cgccttgccg tccttggaga cggtccactt
      301 ctcggccagg tcgggcagga tcgagaagtc ggtgggcgac ttctgctcgg gcccgttggg
      361 gaagcgcacg agctggctgt aggacaggct gatgtaggag tggctcagga agctgatcgt
      421 gaggtgcggg tcgaggcccg gggcctcgcg ctccgcgatg cggatcacgc cgccgcgctt
      481 gggctgggcc cccgactggg ccccggcggg gccggccgcg acgagcgatc cgagcaggag
      541 gccggcgagc agcagaccgg tgaacgagcg ggtacgtcgg gccatggccg gatctcctgt
      601 tctcgagagg gatgtcgtca tcgaccggga gaaggagcga aatgggccga aaagttggcc
      661 tattatcctc ggacgcgggg ggttgtcaat gcgggcgccg agacgtcgca gacgcactcg
      721 ggcggctacc gcggtcctga tcggcctgat ccaccccggc cggctggtat cctgtgcctg
      781 atggccctgg cgcgctcgcg gccggtgatg cagcagatcg ctcccgacaa cccgaagctc
      841 gcggtggtga tggtcgggct gccggcgcgc ggcaagagtt ataccgcccg tcgcatcgag
      901 cgttatctct cgtggctcgg ctaccgcacc ggggtcttca acgtcggcga gtaccggcgg
      961 gcgcggttcg gggcgcatcg gcacagcttc ttcgatcccg acaacgcgac gggcgaggag
     1021 gcgcgctggc gtgccgcgat ggacgccctc gaggacatgg tgcgatggct cgcgggcgac
     1081 ggccaggtcg cgatctacga cgccaccaac accacgcgga ttcgccgtgc cgcggtccgg
     1141 gagtgctgcg agcgcgccgg cttccaggtg ctgttcatcg agatccgctg cgacgacccg
     1201 tccatcatcg aggccaacat ccgcagcacg aagctgtcct cgcccgacta cgaggacgcc
     1261 gatcccgacg aggccaccgc cgatttccgc gcccgcatcg cccactacga gcgggtgtac
     1321 cagccgctcg aggacgacga gggggcgtac ctgcgcgtca ccggcgccgg gcgaggcgtg
     1381 accgccagcg acgtcgacgg ctacctggag gcgcgcctgg tgttcttcct gatgaacctg
     1441 cacctgagcg cgcggccgat ctggctcacc cgtcacggcg agagcgcgta caacgcggcc
     1501 ggtctgatcg gcggtgatcc cgatctgacc ccgggcgggc accgctacgc ccggtcgctc
     1561 gccgagttcc tggaccagcg ctacccggat cacggcgacg tggtgatctg gaccagctcg
     1621 ctccggcgcg cggtgcagac cgcgagcccc ctcgatcgcg ggccctgggc ctggcgcgcg
     1681 ctcgacgaga tcgacgccgg catctgcgac ggcctgagct acgcgcagat ccgggagcag
     1741 ctgcccgagg agtacgaggc acgccggcgc gacaagttca gctaccgcta tccgcggggc
     1801 gagtcgtacc aggacatcat cctgcgcctc gatccggtga tcatcgagct cgagcggacg
     1861 cgccggccgg tgatggtgat cgcccacaac gcggtgctgc gggcgctcta cgcgtatttt
     1921 cagggcgtgc cgcgcgagcg gtgcccgtac ctgaccattc cgctgcacac cgtgatcgag
     1981 ctgacgccgc acgcgtacgg ctgccacgag agccgcgtgc cgctcgagcc gaggctggac
     2041 tcggacgagg cgcggtaggg ggctacctct cggcctcgac cagctccggc gtgtcctgcg
     2101 acggcttgta ccccatgtgc gtcgggaact ccgggctcag cggccaccgc ttcgggaact
     2161 tcagccacag ccgcagcatg tgccgcttct ggtcgggctc ggggccgtcg acgaagccgg
     2221 tgcgggagtg gaggatcgtg tagttgttga tgaactggat gtcgcccgcc tccaggtcca
     2281 tgtccaggcg tagatcgggc cggcgggtct gctcgtcgag gaagtcgagc gcggcgcgct
     2341 cgaaggtggt gagcggcacc tcgcgcttca ccgcgcccgc gttgatctgg ttgcggatgt
     2401 agcggcagct caccaggccg tcctggtagc cgaacaccgg gatcgggcgc tcggagacgc
     2461 cgcgctcggt gagcgccgcc tcgcggcgga tgtagtagta gccgttgtag agcaggccca
     2521 ggtactcggg gtgctgggcc aggatctcgt tgtgcaccgt ggtcgagctg acgatgctgc
     2581 tgagcccgcc ctcgagcgac cggcgcaggc agagcaggcc gaccacgtcg cccgcgtcgg
     2641 tgtgataggg caggtaggcg ttggtctcgt agccgcgcac gtcgatgttg ccgtaggtgc
     2701 ggccgtggtc gtagacgtgg ccgagcagct cgccctgcgg gttctggtag agcggggcgc
     2761 ccatgtagcg gccgaagccc cagaagatga gcccgacgtc ctcgtcgcgg taccggcgca
     2821 ccgggagccc gcgcagcacc aggaagccgc ggccgtcgta catctcggcc actgcctgct
     2881 ccaggcgcgg gcccagcacg gcgagcggga agtgctcgcg ctggatcgac tcgagcggca
     2941 ggccggaggc cttcgcggcg gcgacggcgc ggtcgacgtc ggcgatctcc gcgtcggtca
     3001 gcgtgacgat ccaggaggtc tcgcgggcga gcgtctcgcc gc
//
LOCUS       DATMCV010000916         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_184138, whole genome shotgun sequence.
ACCESSION   DATMCV010000916 DATMCV010000000
VERSION     DATMCV010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184138"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VNU03_20935"
     CDS             complement(<1..374)
                     /locus_tag="VNU03_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP76999.1"
                     /translation="MAARAEFLERIRREVAKTPGLFGASTEPRPPVPAAAAEAIRREL
                     AERWPEALERFRQEFERVAGVFHRVPDLGAVPEVIGDIARERGVRSLVTWDPAVLGFD
                     AASRLSALGCSVAAARAGQEDEA"
     gene            complement(374..1780)
                     /locus_tag="VNU03_20940"
     CDS             complement(374..1780)
                     /locus_tag="VNU03_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HWP77000.1"
                     /translation="MSEGLLQTPFPERASAALKDTFLQEALTIATTKFIDLRKESFAG
                     FPAGEALRDRARAIKEATLQHLDVHLERLADNVERLGGTVHWAATGEEAREIVLRLAR
                     DRGVRMAVKSKSMATEEIDLNEALEHAGITPVETDLGEYIIQLAHEKPSHIIAPAIHK
                     TKGQVAELFSRELRQEFPADPEVLTAVARRVLREKFLQADMGITGANFAVAETGTVVL
                     VTNEGNGRMVTSLPRIHVALMGIEKVIPSMSDLVVFLAILARSATGQKLSSYTTLVRG
                     PRRAGELEGPEEFHLILLDNGRARQIASTLREALYCLRCGACLNVCPVYRQIGGHAYG
                     YTYPGPIGILLTAMLKGNESVRELAHASSLCGACKDVCPVRIDIPRMLIELREHLDRE
                     KIAPWTERMVFRVFRRLLLSPAAFRFSARAGRWLQRPFIRAGRLRGLPLVFGKWTRTR
                     DLPPVAARTFREQWKELR"
     gene            complement(1777..>2171)
                     /locus_tag="VNU03_20945"
     CDS             complement(1777..>2171)
                     /locus_tag="VNU03_20945"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWP77001.1"
                     /translation="QFLVEVLGVSRVDSPFEGRVTYHDSCHLLRGLGESASPRALLRG
                     VPGVELVELPGADQCCGFGGSFSVRLPEVSSAILDRKLANVEATGARCLVACDAGCLM
                     QMGGGLTRRGSTVRAVHLAQVLAGEVSR"
BASE COUNT          312 a          805 c          766 g          288 t
ORIGIN      
        1 gcctcgtcct cctgtccggc gcgggccgcg gccaccgagc agccgagggc ggagagccgc
       61 gacgccgcgt cgaagccgag caccgcggga tcccacgtga cgaggctccg cacgccgcgc
      121 tcccgcgcga tgtccccgat cacctccggg accgcgccca ggtccggcac gcgatggaac
      181 accccggcca cccgctcgaa ctcctgccgg aagcgctcga gcgcttcggg ccagcgctcg
      241 gccagctcgc gccggatcgc ctccgccgcg gccgccggca ccgggggacg gggctcggtg
      301 gaggctccga agagccccgg ggtcttggcc acctcgcggc ggatccgctc gaggaactcg
      361 gcgcgggcgg ccactagcgc agctccttcc actgctcgcg gaaggtgcgg gccgccaccg
      421 ggggcaggtc ccgtgtgcgc gtccacttcc cgaacacgag cggcagcccg cgcagccgcc
      481 ccgcgcggat gaacggccgc tggagccacc ggcccgcgcg cgcggagaag cggaacgcgg
      541 cgggcgagag cagcaggcgc cggaagaccc ggaacaccat ccgctccgtc cagggcgcga
      601 tcttctcccg gtcgaggtgc tcgcgcagct cgatcagcat gcgcgggatg tcgatgcgca
      661 ccgggcagac gtccttgcac gccccgcaaa gggaggaggc gtgggccagc tcgcgcaccg
      721 actcgttgcc cttgagcatc gcggtgagga ggatgccgat gggccccggg tacgtgtagc
      781 cgtaggcgtg gccgccgatc tggcggtaca ccgggcagac gttgaggcac gccccgcagc
      841 gcaggcagta gagcgcctcg cgcagggtcg aggcgatctg ccgcgccctc ccgttgtcga
      901 ggaggatcag gtggaattcc tccggccctt cgagctcccc cgcccgccgc ggtccccgca
      961 ccagcgtggt gtaggaggag agcttctggc cggtcgcgct gcgggcgagg atggcgagga
     1021 acaccaccag gtcgctcatc gaggggatga ccttctcgat ccccatcagg gccacgtgga
     1081 tccggggcag cgaggtcacc atgcgcccgt tgccctcgtt ggtgaccagc accaccgtgc
     1141 cggtctccgc caccgcgaag ttggccccgg tgattcccat gtcggcctgg aggaacttct
     1201 cgcgcaggac ccggcgggcc accgcggtca ggacttccgg atcggcagga aactcctgac
     1261 gcagctcgcg cgagaacagc tccgccacct gccccttcgt cttgtggatc gcgggggcga
     1321 tgatgtgcga cggcttctcg tgggcgagct ggatgatgta ctcgcccagg tcggtctcca
     1381 ccggggtgat gcccgcgtgc tcgagggcct cgttgaggtc gatctcctcg gtggccatcg
     1441 acttggactt caccgccatc cgcaccccgc ggtcgcgcgc gagccgcagc acgatctcgc
     1501 gcgcttcctc cccggtggcg gcccagtgca ccgtcccgcc gagccgctcc acgttgtcgg
     1561 cgagccgctc caggtggacg tcgaggtgct gcagggtggc ctccttgatg gcccgcgccc
     1621 ggtcccgcag cgcctcgccc gccgggaagc ccgcgaagga ctccttccgc aaatcgatga
     1681 acttggtggt ggcgatcgtg agcgcttcct gaaggaacgt gtccttgagc gccgcgctgg
     1741 cgcgctcggg gaacggggtc tgcaggagcc cctcgctcac cgcgagacct cgccggccag
     1801 gacctgggcc aggtgcaccg ctctcaccgt cgagccccgg cgagtcaggc caccgcccat
     1861 ctgcatgagg cagcccgcgt cgcaggccac gaggcagcgc gcgccggtgg cctcgacgtt
     1921 ggccagcttc cggtcgagga tggcgctcga cacctcgggc aagcgcaccg agaacgaccc
     1981 cccgaagccg cagcactgat ccgcgcccgg cagctcgacc agctccacgc cgggcacccc
     2041 gcgcagcagg gcgcgcggcg aggcggattc tccgaggccg cgcaggaggt ggcaggagtc
     2101 gtggtaggtc accctgccct cgaagggcga gtcgacgcgg ctcacgccca gcacctccac
     2161 caggaactgg g
//
LOCUS       DATMCV010000917         2522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_116238, whole genome shotgun sequence.
ACCESSION   DATMCV010000917 DATMCV010000000
VERSION     DATMCV010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116238"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /gene="recF"
                     /locus_tag="VNU03_20950"
     CDS             complement(<1..944)
                     /gene="recF"
                     /locus_tag="VNU03_20950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HWP77002.1"
                     /translation="MQIDRVELSDFRNYRSLSLSPAPHLNVLTGLNAQGKTNLLEALG
                     VLLLGRSFRGAKAADMLGWDATTAFVGGEIRRGDTVRVVRRAVEQREDGSWGIGGEGC
                     PWARAIGFGWQDLAILTEGPQARRNFIDGFAGKLYAAHLSGLARYRQVLARRNHLLQS
                     GMVGCALRSALTPWSEQLARVGLELVARRRSALEALQEEVRALYPTLAGSGDVTLRYS
                     RGLPADATAADVLAAFDEHFAEETRRGQTVVGPHRDDLLIEVDERDLRLYGSRGQQRL
                     MALALRLAEAGPVARAIGSAPVLLLDDALSELDPQVQAR"
     gene            complement(967..2061)
                     /gene="dnaN"
                     /locus_tag="VNU03_20955"
     CDS             complement(967..2061)
                     /gene="dnaN"
                     /locus_tag="VNU03_20955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWP77003.1"
                     /translation="MEVHVDRDAFIRGLQMAHNVVEPRQTLPVLANVLLEAQGDTLRM
                     TATDLEVSVRVTGPAKVVKPGTITISARKLMEIVKELPAAPLAIKVQENAWVALRCAG
                     ASYRLVGLTPEEFPAVGDGASTGWLTLDGKVIREMLSQTTFAISHDESRYALNGVLLA
                     VQDKELRMVATDGHRLALAVRPLTAAGSAMSGIVPRKAVQEVARIVGSGEEVQIAISD
                     NQFMLRMPNVLLMARLIEGTFPNYEQVVPRAHPHRVTLSRAALTAALRRVSVLSEERT
                     KPVRFLFSPGLLKLTANSPDYGEAEEQMEAQFAGEEIAIGFNSRYVLDTLGAQAGEQV
                     LIELKDGLSPGVFKSFEDEGTLCVIMPMRI"
     gene            complement(2229..>2522)
                     /locus_tag="VNU03_20960"
     CDS             complement(2229..>2522)
                     /locus_tag="VNU03_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP77004.1"
                     /translation="EKIITIEHIQRKVAEFFGVKLGDMRAKNRTKAVAFPRQIAMYLA
                     RQLTHASLAEVGRMFGGKDHTTVLHAVEKIHGMLQDDPKFKKTVDTLTQSITL"
BASE COUNT          407 a          871 c          831 g          413 t
ORIGIN      
        1 cgggcctgca cctggggatc gagctcggag agcgcgtcgt cgagcagcag caccggcgcg
       61 ctgccgatcg cgcgcgccac cggccccgcc tcggccaggc gaagcgcgag cgccatcagc
      121 cgctgctgcc cgcgcgagcc gtagagccgc agatcgcgct cgtccacctc gatcagcaga
      181 tcgtcgcgat gcggcccgac caccgtctgg cctctccgcg tctcctcggc gaagtgctcg
      241 tcgaacgcgg ccaggacgtc ggccgcggtc gcgtccgccg ggaggcccct gctgtatcgc
      301 agggtcacgt cccccgatcc ggccagcgtc gggtagagcg cgcggacctc ctcctggagg
      361 gcctccagcg cgctgcggcg tcgggccacc agctcgaggc cgacccgcgc gagctgctcg
      421 ctccacgggg tgagcgcgct gcggagcgcg catcccacca tcccgctctg gagcagatga
      481 ttgcgccggg ccagcacctg gcgatagcga gcgagaccac tcaggtgggc cgcgtagagc
      541 ttgcccgcga agccgtcgat gaagttgcgg cgcgcctggg gcccctcggt caggatcgcg
      601 aggtcctgcc agccgaagcc gatcgcgcgc gcccaggggc agccttcgcc gccgatcccc
      661 cagctgccgt cctcacgctg ctcgacggcg cggcgcacca cgcgcaccgt gtcaccccgc
      721 cggatctcgc cacccacgaa cgcggtggtc gcgtcccagc cgagcatgtc ggccgccttc
      781 gcgccgcgaa acgagcgccc gagcagcagg acgccgagtg cctcgagaag gttcgtcttc
      841 ccctgcgcgt tcagtccggt gaggacgttg aggtggggcg ccggcgagag cgagaggctt
      901 cgatagttgc gaaaatcaga cagttctacc ctgtcaatct gcaacgattt tcgctcccat
      961 gagaggtcaa atcctcatgg gcatgataac acacagggtt ccctcgtcct cgaagctttt
     1021 gaacactccg gggctcaaac cgtccttcaa ttcgatcaaa acctgctcgc ccgcctgcgc
     1081 gccgagcgtg tcgagcacgt agcgcgagtt gaagccgatc gcgatctcct cgcccgcgaa
     1141 ttgcgcctcc atctgctcct ccgcctcacc atagtcgggg ctgttggcgg tgagcttgag
     1201 caacccgggc gagaacagga accgcaccgg cttcgtgcgc tcctccgaca ggaccgatac
     1261 gcgccgcagg gcggcggtca gcgcagcccg ggagagcgtg acccggtggg gatgggcccg
     1321 cgggaccacc tgctcgtagt tcgggaaggt cccctcgatc aggcgcgcca tcagcagcac
     1381 gttgggcatc cgcagcatga actggttgtc gctgatcgcg atctgcactt cctcgcccga
     1441 gccgacgatg cgcgcgacct cctggaccgc cttccgcggg acgatcccag acatggcgga
     1501 gccggccgcg gtgagcggcc gcaccgcgag cgcgagccga tggccgtcgg tggccaccat
     1561 ccgcagctcc ttgtcctgga ccgccagcag gaccccgttc agcgcatacc ggctctcgtc
     1621 gtgcgagatc gcgaaggtgg tctgcgagag catctcgcga atcaccttgc cgtcgagggt
     1681 cagccagccg gtactcgccc cgtccccgac cgcgggaaat tcttccgggg tgaggcccac
     1741 gagccggtag gacgcccccg cgcaccgcag ggcgacccac gcgttctcct gcaccttgat
     1801 ggcgagcggg gccgccggca gctccttcac gatctccatg agcttgcgcg cggatatcgt
     1861 gatggtgccc ggcttcacca ccttcgcggg cccggtcacc cgcacgctga cctcgaggtc
     1921 ggtggcggtc atgcgcagcg tgtcgccctg tgcctcgagc agcacgttcg ccagcaccgg
     1981 cagcgtctga cgcggctcga ccacgttgtg cgccatctgg agaccacgga tgaaagcatc
     2041 acgatccaca tgcacttcca taagactctc cgttgttcag tgattgaaga ggaaaagaaa
     2101 ctttcgtcgt cgtacgagta ggcggagcgg tggacaagaa cggagccgga taaaggcgcg
     2161 cgacggcgtc gagttatgag cggcgccatc gtggacaagt cggtgggtac ccgggaggtg
     2221 ccggaggatc acagggtgat gctctgggta agtgtgtcga cggtcttctt gaatttggga
     2281 tcatcctgca gcatgccgtg aatcttctcg accgcgtgga gcacggtggt gtgatccttg
     2341 ccgccgaaca tccgcccgac ctcggcgagc gaggcgtggg taagctggcg cgcgaggtac
     2401 atcgcgatct gtcgcgggaa ggcgaccgct ttggtccggt tcttggcgcg catgtcgccg
     2461 agcttgaccc cgaagaactc ggcgactttc cgctggatat gctcgatcgt gatgatcttc
     2521 tc
//
LOCUS       DATMCV010000918         2347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_648339, whole genome shotgun sequence.
ACCESSION   DATMCV010000918 DATMCV010000000
VERSION     DATMCV010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648339"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VNU03_20965"
     CDS             complement(<1..370)
                     /locus_tag="VNU03_20965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77005.1"
                     /translation="MTAARILVVDDEADISTILTVTLRRAGYEVASAADGIEAIEAIH
                     RQAPDLILLDVMMPRSDGLETLKRIRQHPPTAQVPVIMLTARAGLADKMKGFERGADD
                     YVAKPFEPAEIVVRVAALLKR"
     gene            complement(367..>2347)
                     /locus_tag="VNU03_20970"
     CDS             complement(367..>2347)
                     /locus_tag="VNU03_20970"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77006.1"
                     /translation="YSATQQNLAGAALLNAAARTLHRTLDVKRLLPEAAGTLGQTFGA
                     SRVGIVLFEEPGSGSGDVIAWGDWPPEAVRALAEPLRRREAPLLIPDTGARADLLRAG
                     LVEPGGGLAGFPVRGRSRVLGGLLLQFAGPRALTEAETRLLAAYADQLAMALDNATLF
                     ENAENQKTQLEQVFASTSDGFLVLDLDREVVALNQRGGDLLGIAPHAVIGRPFARLVE
                     AIGQAVDWDAAGGPALAAALERAGESADGDLELAGLDRRTLRWMITPTRDLLGDEVGV
                     TVSLRDVTREREIDRMKTEFVSTVSHELRTPLASIKGSLHLLLSDEHLVLDETQRRLV
                     DISLKNTDRLIRLINNILDISKMEAGQIHLDLEMHQPVDFVELAVDGIRGFAESRRIS
                     IETQLEAELPSVRVDFDRMVQVVTNLLSNAIKFSPEQERVTVGARRAGVDLEIWVSDH
                     GRGIAPEDVGRLFRKFQQLDGSNVRAVGGTGLGLAICRGIVEEHGGRIGVDSRVGAGA
                     TFTVRLPVPGAAVAPGAEAPAEGKEQAPLVLVVDDEPDVRALMRDVLQLSGFRVIEAG
                     RVLEGVELARQQRPDVITMDLMLPDLDGFEAIRLLREQPETRDTPVLVLSAIEVEPND
                     PRALGATVCLTKPFSSADLLGAIRSRLPAPNGGGA"
BASE COUNT          309 a          869 c          844 g          325 t
ORIGIN      
        1 agcgcttgag cagggcggcc acccggacca cgatctcggc cggctcgaag ggcttggcca
       61 cgtaatcgtc ggcgcctcgc tcgaagccct tcatcttgtc ggcgaggccg gcccgcgcgg
      121 tcagcatgat gaccggcacc tgcgcggtgg gcgggtgctg ccggatgcgc ttgagggtct
      181 cgagcccgtc ggagcgcggc atcatcacgt cgagcaggat caggtcgggc gcctgccggt
      241 ggatggcctc gatggcctcg atcccgtccg ccgcgctcgc gacctcgtag ccggcgcgcc
      301 tcagggtcac ggtcaggatg gtgctgatgt ccgcctcgtc gtcgacgacg aggatgcgcg
      361 ccgcggtcac gcgccgccgc cgttcggagc cggcaggcgc gagcggatcg cgccgagcag
      421 atcggccgag ctgaacggct tggtgaggca gacggtggcc ccgagcgcgc gcggatcgtt
      481 cggctcgacc tcgatcgcgc tcagcaccag caccggggtg tcccgcgtct cgggctgctc
      541 ccgcaggagc cggatggcct cgaagccgtc gaggtcgggc agcatgaggt ccatcgtgat
      601 cacgtcggga cgctgctgcc gcgccagctc gacgccctcg agcacgcgcc ccgcctcgat
      661 gacgcggaaa ccggagagct ggaggacgtc gcgcatgagc gcgcggacgt cgggctcgtc
      721 gtccaccacc agcaccagcg gcgcctgctc cttcccctcg gcgggagcct ccgcgccggg
      781 ggcgacggcc gcgcccggca ccggcaggcg caccgtgaag gtggcgcccg ccccgacccg
      841 gctgtccacc ccgatgcgcc cgccgtgctc ctccacgatc ccgcggcaga tcgcgaggcc
      901 gaggccggtc ccgccgaccg cccgcacgtt gctcccgtcg agctgctgga acttgcggaa
      961 gagccggccc acgtcctccg gcgcgatgcc ccgcccgtgg tcgctcaccc atatctccag
     1021 atccacgccc gcccggcggg cccccaccgt gacccgctcc tgctcgggtg agaacttgat
     1081 cgcgttcgag aggaggttgg tcaccacctg gaccatgcgg tcgaagtcga cgcgcaccga
     1141 cggcagctcc gcctcgagct gggtctcgat cgagatccgg cgcgactccg cgaacccgcg
     1201 gataccatcc accgccagct cgacgaagtc gacgggctgg tgcatctcga ggtccaggtg
     1261 gatctggccg gcctccatct tggagatgtc gaggatgttg ttgatgagcc ggatgagccg
     1321 gtcggtgttc ttgagcgaga tgtcgacgag ccggcgctgg gtctcgtcga gcacgaggtg
     1381 ctcgtcggac agcaggaggt gcagcgaccc cttgatcgac gcgagcggcg tgcgcagctc
     1441 gtgcgagacg gtggagacga actcggtctt catgcggtcg atctctcgct cgcgggtgac
     1501 gtcgcgcagg ctcaccgtga ccccgacctc gtcccccagc aggtcgcggg tgggcgtgat
     1561 catccagcgg agcgtccgcc ggtccaggcc cgccagctcg aggtcgccgt cggcgctctc
     1621 ccccgcccgc tcgagcgcgg cggccagggc cggcccgccc gcggcgtccc agtccaccgc
     1681 ttgcccgatc gcctccacga ggcgggcgaa cgggcggccg atcaccgcgt ggggggcgat
     1741 cccgagcagg tcgccgccgc gctggttgag cgccaccacc tcccggtcga ggtcgagcac
     1801 caggaagccg tcggaggtcg aggcgaagac ctgctcgagc tgggtcttct ggttctccgc
     1861 gttctcgaac agcgtcgcgt tgtcgagcgc catcgcgagc tggtccgcgt aggccgcgag
     1921 gagccgggtc tcggcctcgg tgagcgcgcg cgggcccgcg aactggagga gcagcccgcc
     1981 cagcacgcgg ctccgtcccc gcaccgggaa gccagccagc ccgccgcccg gctccaccag
     2041 cccggcccgg agcaggtccg cgcgggcccc ggtgtcggga atcagcaacg gcgcctcgcg
     2101 gcggcggagc ggttcggcga gcgcgcgcac tgcctcggga ggccagtcgc cccacgcgat
     2161 gacgtcgccg ctcccgctcc ccggctcctc gaacagcacg atgcccaccc ggctcgcccc
     2221 gaaggtctgc ccgagggtcc cggccgcctc gggcagcagc cgcttgacgt cgagcgtgcg
     2281 gtgcagcgtg cgggcggccg cgttgagcag ggccgcgccg gccaggttct gctgggtggc
     2341 cgagtac
//
LOCUS       DATMCV010000919         3946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_584947, whole genome shotgun sequence.
ACCESSION   DATMCV010000919 DATMCV010000000
VERSION     DATMCV010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584947"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VNU03_20975"
     CDS             complement(<1..884)
                     /locus_tag="VNU03_20975"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP77007.1"
                     /translation="MIVVPDDFPSVFEGSEGHERAKRLGPVRVFTERGADQEAELIRR
                     IGDATSAINIRAHARFSEGVFQACPKLKMVSVWGTGTDNLDLNAAGMRGVTICNTPGV
                     NAFAVAEHALTLMLAVARRVVTTDAEMRKGRWPRELMTQCVGKTLGVFGMGAIGGRLA
                     TIASGIGMNVLGWSALGDEARIRKAGARPASKEEILAQADVVSLHLRLSTETRNFLGR
                     KELAMMKPTAIIVNTGRGALVDREALLAALRDRKIMGAGLDVFHQEPLAADDPILALP
                     NVVVSPHNAGQTPEVIRDG"
     gene            complement(881..2107)
                     /locus_tag="VNU03_20980"
     CDS             complement(881..2107)
                     /locus_tag="VNU03_20980"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWP77008.1"
                     /translation="MDATIRWGTTQSQPILMIPGPTELPFPVVQAMSQPATIQYDLNF
                     DVKILEPVTLALRDIFQTSRGEVIVMPGSGRTALEAAAISVIEPGDRVLVVVAGVFGS
                     LMREIMTRAGAEVTEHIVEWGRPLDLARLEKDVERVKPKAVSMVHNETSTGTTYPAAE
                     VGRIVKRHGALFMLDTVSSLAGLDVRTDDWGVDFNMTGSQKCLAAPLGMAVVSVSPAA
                     WEAMERRKHKSSSWAYDLLRWKENWIPVSRGGHIPDGSPRRQPVSIPTHLTNALGVAS
                     RLILEEGLAHRFRRHAVAGRAFRAGLEAMRLELFPDASLLSNTVSCFKAPAGIDPGAV
                     VSHMRDRYGILIGTGLDKIRTTTLRVGHMGITSSPMYVLPTLSAIEMTLRDLGYRSEA
                     GAGVSAAQAVFSSPPA"
     gene            complement(2137..2421)
                     /locus_tag="VNU03_20985"
     CDS             complement(2137..2421)
                     /locus_tag="VNU03_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HWP77009.1"
                     /translation="MTSEHFWLTFGFLGQAFFSMRFLVQWIASERKKESVIPVSFWFF
                     SIGGGLTLLVYALYRMDPVFILGQGAGLFVYLRNLYLIRRKQSGLAEARA"
     gene            2519..3001
                     /locus_tag="VNU03_20990"
     CDS             2519..3001
                     /locus_tag="VNU03_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77010.1"
                     /translation="MRGIDQALVSEVWREITSYPPGRIDEEARDFLSRQPEVAAFAHT
                     VTAEQDPVVQRAGLGLCFLLFKILERSLGHPFPRLEESRLRAAHDASTEWLAGLEGDD
                     SARILAAAGAPGHPTLAAHLVAAFYGEGAEACDERVRAGFLLMLRTLTDALDLGPVEA
                     "
     gene            3001..3852
                     /locus_tag="VNU03_20995"
     CDS             3001..3852
                     /locus_tag="VNU03_20995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP77011.1"
                     /translation="MPRVAVNGLALHYDEMGAGPPLVLIHAFPVGRRMWEAQMTALAR
                     RHRVIAYDVRGFGLSDAPREPGAYSQAQSVEDARALIEALGAAPAAVCGLSMGGNIAL
                     NLALMYPETVSALLLCDTGAGSEAPAVFAARCEEYAAAADRGIEAFFDAALTWPVFAD
                     YGAQGGRQRAFLRELILGHPAHGIALTARHALATRKPVYALEAGMRALRVPTLVAFGE
                     RDEACVRSSEFMAATIPDARLWRVPGGTHFINLDEPELFNRAVRARAGVAEQQRGDCL
                     RVHQGPG"
BASE COUNT          555 a         1370 c         1436 g          585 t
ORIGIN      
        1 ccgtcgcgga tgacctcggg ggtctgcccc gcgttgtgcg gggagaccac cacgttgggc
       61 agggccagga tcggatcgtc ggcggcgagc ggctcctggt ggaagacgtc gaggccggcg
      121 cccatgatct tgcgatcgcg cagggctgcc agcagggcct cccgatccac cagcgcgccg
      181 cggccggtgt tcacgatgat ggcggtgggc ttcatcatcg ccagctcctt gcggccgagg
      241 aagttgcgcg tctcggtgga gaggcgcagg tgcaggctga ccacgtcggc ctgggccagg
      301 atctcttcct tgctggcggg ccgggccccg gccttccgga tccgcgcctc gtcgccgagc
      361 gcgctccagc cgagcacgtt catgccgatg ccactcgcga tggtggcgag ccgtccgccg
      421 atcgccccca tgccgaagac gcccagggtc ttgcccacgc actgggtcat cagctcccgc
      481 ggccagcggc ccttgcgcat ctccgcgtcg gtggtgacca cacggcgcgc caccgcgagc
      541 atcagggtga gcgcgtgctc ggccaccgcg aaggcgttga ccccgggggt gttgcagatc
      601 gtgaccccgc gcatgcccgc cgcgttcagg tcgaggttgt cggtgccggt gccccacacc
      661 gagaccatct tgagcttggg gcaggcctgg aacacgccct cgctgaagcg ggcgtgggcg
      721 cggatgttga tggcgctggt ggcgtcgccg atgcggcgga tcagctccgc ctcctggtcc
      781 gcgccgcgct cggtgaagac gcggaccggc ccgaggcgct tggcgcgctc gtggccctcg
      841 ctgccctcga agacggaggg gaagtcgtcc gggacgacga tcacgcgggc gggctcgaga
      901 agaccgcctg ggccgcggag acccccgcgc cggcctcgct gcggtagccg aggtcgcgca
      961 gcgtcatctc gatcgcggac agggtgggca gcacgtacat cgggctcgag gtgatgccca
     1021 tgtgcccgac ccgcagggtg gtggtgcgga tcttgtcgag cccggtgccg atcaggatgc
     1081 cgtagcgatc gcgcatgtgg ctcaccaccg cgcccgggtc gatgccggcc ggggccttga
     1141 agcacgacac ggtgttggac aggagcgagg cgtccgggaa cagctcgagc cgcatcgcct
     1201 cgaggccggc gcggaaggcg cgtccggcca ccgcgtgacg gcggaagcgg tgggcgaggc
     1261 cctcctcgag gatgagccgg ctcgccaccc ccagcgcgtt cgtgaggtgg gtcgggatcg
     1321 agaccggctg ccggcgcggg ctgccgtccg ggatgtggcc gccgcgcgag acggggatcc
     1381 agttctcctt ccagcgcagc aggtcgtagg cccacgacga ggacttgtgc ttgcggcgct
     1441 ccatcgcctc ccacgcggcg gggctgaccg acaccaccgc catcccgagc ggcgcggcga
     1501 ggcacttctg cgagccggtc atgttgaagt cgacgcccca gtcgtcggtg cggacgtcga
     1561 gcccggcgag agacgacacg gtgtcgagca tgaagagggc gccgtgccgc ttcacgatgc
     1621 gtcccacctc cgcggcgggg taggtggtgc cggtggaggt ctcgttgtgc accatggaga
     1681 ccgccttggg cttcacgcgc tcgacgtcct tctcgagccg ggcgagatcg agcgggcgtc
     1741 cccactccac tatgtgctcg gtcacctcgg cgcccgcgcg ggtcatgatc tcgcgcatca
     1801 gggagccgaa gaccccggcc accacgacga ggacgcgatc gccgggctcg atcaccgaga
     1861 tcgcggcggc ctcgagcgcg gtccggcccg agccgggcat gacgatgacc tcgccgcggc
     1921 tcgtctggaa gatgtcgcgc agcgcgaggg tgaccggctc gaggatcttg acgtcgaagt
     1981 tcaggtcgta ctggatggtg gcgggctggc tcatcgcctg cacgaccggg aacggcagct
     2041 cggtcgggcc ggggatcatc aagatcggct gcgactgggt cgtcccccag cggatggtgg
     2101 cgtccatgaa gactcctttg agtgacgtgg aggcggctag gccctggcct cggccaggcc
     2161 gctctgcttc cgccggatca gatagaggtt gcgcagatac acgaagaggc ccgcgccctg
     2221 accgaggatg aagaccggat ccatgcggta cagcgcgtag acgagcaggg tgagtccgcc
     2281 gccgatcgag aagaaccaga aggagaccgg gatcacgctc tccttcttcc gctcggacgc
     2341 gatccactgc acgaggaagc gcatcgagaa gaaagcctgt cccaggaacc cgaacgtcag
     2401 ccagaaatgc tcggacgtca tcatcgcccc gtctcctcgg tggttcgtcg catcatgcta
     2461 gaatcacagc cactcacatt atcggaccta cccgcgggtc gcaaggagtt tcgtcaccgt
     2521 gcgaggcatc gaccaggcgc tggtgagcga ggtatggcgt gagatcacgt cgtatccccc
     2581 cgggcggatc gacgaggagg cgcgcgactt cctgtcgcgc cagccggagg tggccgcctt
     2641 cgcgcacacc gtcaccgccg agcaggatcc tgtcgtgcag cgcgcgggtc tcggcctctg
     2701 cttcctgctc ttcaagatcc tcgagcggag tctggggcac ccgttcccgc ggctcgagga
     2761 atcccgcctg cgggcggcgc acgacgcctc gacggaatgg ctcgccggcc tcgagggcga
     2821 cgactcggcg cggattctcg ccgcggccgg cgcgcccggg cacccgacgc tcgcggccca
     2881 cctggtggcg gcattctacg gcgagggcgc ggaggcgtgc gacgagcggg tgcgggccgg
     2941 cttcctgctc atgctgcgca ccctgaccga cgcgctggat ctgggcccgg tcgaggcctg
     3001 atgccgcggg tcgcggtcaa cgggctcgcg ctccactacg acgagatggg cgccggcccg
     3061 ccgctcgtgc tgatccacgc ctttccggtc gggcggcgga tgtgggaggc gcagatgacg
     3121 gcgctcgcgc ggcggcaccg ggtcatcgcg tacgacgtgc ggggcttcgg tctgtcggat
     3181 gccccgcgcg agccgggcgc ctactcccag gcccagtcgg tggaggacgc tcgcgccctg
     3241 atcgaggccc ttggcgccgc gccggccgca gtgtgcgggc tcagcatggg cggcaacatc
     3301 gcgctcaacc tggccctgat gtacccggag acagtctccg cgctgctgct ctgcgacacc
     3361 ggcgcgggct cggaagcccc ggccgtgttc gcggcccgct gcgaggagta cgcggcggcc
     3421 gccgaccggg gcatcgaggc cttcttcgac gcggcgctca cgtggccggt gttcgcggat
     3481 tacggggccc agggcggccg ccagcgcgcg ttcctgcggg agctcatcct cggccatccg
     3541 gcccacggca tcgccctcac cgcccgccac gcgctggcca cccgcaagcc ggtctacgcg
     3601 ctcgaggcgg gcatgcgcgc gctgcgcgtg cccaccctgg tcgcctttgg cgagcgggac
     3661 gaggcgtgcg tgcgcagctc cgagttcatg gccgcgacga tcccggacgc gcggctctgg
     3721 cgcgtgcccg gcgggaccca cttcatcaac ctcgacgagc ccgagctctt caaccgcgcg
     3781 gtgcgagccc gcgccggtgt cgcagagcag cagcgcggag actgtctccg ggtacatcaa
     3841 gggccaggtt gagcgcgatg ttgccgccca tgctgagccc gcacactgcg gccggcgcgg
     3901 cgccaagggc ctcgatcagg gcgcgagcgt cctccaccga ctgggc
//
LOCUS       DATMCV010000920         4252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_783234, whole genome shotgun sequence.
ACCESSION   DATMCV010000920 DATMCV010000000
VERSION     DATMCV010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783234"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VNU03_21000"
     CDS             complement(<1..1404)
                     /locus_tag="VNU03_21000"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77012.1"
                     /translation="MLRVELLIVLLLLLGAAPAVAQGPAGEVAVSFHVTLAPTWFDPS
                     TAPAQITPFGVIYAIHDALVRPLPGQKMGASLAESWTESADGLVYEFRLRRGLKFHNG
                     DPLTTDDVKWSFERYQGAGAREFRARVRQVEVVDPTVIRFYLKEPWPDFMTFYGTTAT
                     AAGIVLPRKYLEQVGADGFRQRPVGAGPYKFVSQRPGIDITLEAFPGYWRHAPYVKRL
                     VMKSVPDSTTRLTMLKSGETDYALFLDGPEAEAVTRDPRYQLVATRHASIFWIEFADQ
                     WDPKSPWADRRLRLAVNHALDRKGTNEAACLGHCPPTGVFVPRVMDFALQVPPPAYDP
                     AKAKQLLAEAGFPRGIDAGTFTAIPGFSTVGDAVLNNLNAVGIRVRMRPTERAAFYAD
                     WQEKKLRGLFMVAVGNSGNAASRIESFVYSKGAAAYGGYPDLDELFLQQARERDPARR
                     EALLHRIQQATVDRVMFA"
     gene            complement(1427..2482)
                     /locus_tag="VNU03_21005"
     CDS             complement(1427..2482)
                     /locus_tag="VNU03_21005"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HWP77013.1"
                     /translation="MATRALAGQAQTVLGPIAGDAMGITLPHEHLLIDFEVMFREPAT
                     GAERGLSRQPVSLANLGWVRHHFSSNLDNLQLLDETVARDEALLFKHAGGQTFVDPTN
                     RGLARDPLALARVARATGLNIIMGSGYYVAAAHPPGMDTRTVDDIARELVTDLTVGVD
                     GTGVRAGFIGEIGTTWPWTDNEKKVVRAAVAAQRETGAALMIHPGRHERLPLAIVDFI
                     RKEGADLERTIMCHIERTIADRGVVLELAATGVRLEYDLFGLETSYYPYNPAFDMPND
                     GERMRQILFLIERGHLAQILMSHDIAYKHCLTKWGGFGYHHLLVNVIPRLRDRGADDR
                     TIQTLLVDNPRRAFTYA"
     gene            complement(2483..3256)
                     /locus_tag="VNU03_21010"
     CDS             complement(2483..3256)
                     /locus_tag="VNU03_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP77014.1"
                     /translation="MAEPHPLDRRRFVLRGLALAAAPLLAGCDRLSGTPWVARLLHAG
                     EAVTHRVQRTLTRRGALAREFPESARSAVFPGNGTIDPGTDAYQATATAGFADWRLEV
                     RGLVERPARLSLRELRRLPARTQVTRHDCVEGWSAIAKWTGVRMSALLDAAGVRPEAR
                     YVVLRCADLMAGSGEAYYESIDLVDAYHEQTILAYEMNDQPLPVAQGAPLRLRVERQL
                     GYKMAKYVMAVELVDRLEHIARGRGGYWEDRGYEWYAGI"
     gene            complement(3249..4073)
                     /locus_tag="VNU03_21015"
     CDS             complement(3249..4073)
                     /locus_tag="VNU03_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWP77015.1"
                     /translation="MTDGPASRTWLYRHTAATRLSHWITVLCLLVLVPSGLQIFNAHP
                     ALYWGERSDPEHAVFALRAIEIGDDEIRGVAELWGHRLDTTGLLGASTEDGERVARGF
                     PAWMTLPGSQWLAMGRRWHLFFAWIFVLNALTFGVYALLSRHLTRDLLPDGRDLAGLG
                     ASLRDHLRFRFPAGEPRYNVLQKAAYVTVIAVLGPLVVLTGLAMSPWADATLPLLPAL
                     LGGRQSARTVHFALTWALVGFTVMHVFMVAATGVLNNLRAILTGWARVHHPGGSDG"
BASE COUNT          610 a         1443 c         1616 g          583 t
ORIGIN      
        1 ggcgaacatc acccggtcca ccgtggcctg ctggatccgg tgcagcaggg cctcgcggcg
       61 ggccgggtcc cgctcgcgcg cctgctgcag gaagagctcg tcgagatcgg ggtagccgcc
      121 gtaggccgcc gcgcccttcg agtagacgaa cgattcgatg cggctcgccg cgttgccgga
      181 gttgcccacc gcgaccatga agagcccacg gagcttcttc tcctgccagt cggcgtagaa
      241 ggcggcgcgc tcggtcggac gcatccgcac ccggatgccg accgcattca aattgttgag
      301 cacggcgtcg cccacggtgg agaagccggg gatcgcggtg aaggtgcccg cgtcgatgcc
      361 gcgcgggaag ccggcctccg cgaggagctg cttggccttc gcggggtcgt aggcgggtgg
      421 cgggacctgc agcgcgaagt ccatcacccg cggcacgaag accccggtgg gcgggcagtg
      481 gccgaggcag gccgcctcgt tggtgccctt gcggtcgagc gcgtggttga ccgccaggcg
      541 caggcgccgg tcggcccagg gcgacttggg atcccactgg tccgcgaact cgatccagaa
      601 gatcgacgcg tggcgcgtgg ccacgagctg gtaccgcgga tcgcgcgtca ccgcctccgc
      661 ctcgggaccg tccaggaaga gcgcgtagtc ggtctcgccg ctcttgagca tggtcaggcg
      721 ggtggtgctg tccggcacac tcttcatgac gagtcgcttg acgtagggcg cgtgacgcca
      781 gtaccccggg aaggcctcga gcgtgatgtc gatccccggc cgctggctca cgaacttgta
      841 cgggcccgcg cccaccggcc gctggcggaa gccgtcggcc ccgacctgct cgaggtactt
      901 gcggggcagg acgatcccgg ccgcggtggc ggtggtcccg tagaaggtca tgaaatcggg
      961 ccacggctcc ttcagataga agcggatcac cgtcgggtcg acgacctcca cctggcgcac
     1021 gcgggcccgg aactcgcgcg cgcccgctcc ctggtagcgc tcgaagctcc acttcacgtc
     1081 gtcggtggtg agcggatcgc cgttatggaa cttgaggccg cgccggagcc ggaactcgta
     1141 gacgagcccg tcggcgctct cggtccacga ttcggcgagg ctcgcgccca tcttctgacc
     1201 cgggagcggg cggaccaggg cgtcgtggat cgcgtagatc actccgaagg gcgtgatctg
     1261 ggccggcgcg gtcgagggat cgaaccaggt cggggccagc gtgacgtgga aggagaccgc
     1321 gacctcgccc gcgggaccct gggccacggc cggcgccgcg cccagcagga gcagcaacac
     1381 gatcaggagc tcgactctta gcatcggcgg ccctcctcgt gacccctcag gcgtaggtga
     1441 aggcccggcg ggggttgtcg accagcaagg tctggatggt ccggtcgtcg gcgccccggt
     1501 cgcgcaggcg cgggatcacg ttgacgagca ggtggtgata gccgaagccg ccccacttcg
     1561 tcaggcagtg cttgtaggcg atgtcgtgcg acatgaggat ctgggccagg tggccgcgct
     1621 cgatcaggaa caggatctgc cgcatccgct cgccgtcgtt cggcatgtcg aaggccgggt
     1681 tgtacgggta gtacgaggtc tcgaggccga agaggtcgta ctcgaggcgc accccggtcg
     1741 cggccagctc gagcaccacg ccgcggtcgg cgatggtgcg ctcgatgtgg cacatgatcg
     1801 tgcgctcgag gtcggcgccc tcttttctga tgaagtcgac gattgcgagc ggcagccgct
     1861 cgtggcggcc ggggtggatc atgagggcgg cgccggtctc gcgctgggcc gccaccgccg
     1921 cccgcaccac cttcttctcg ttgtcggtcc agggccacgt ggtgccgatc tcgccgatga
     1981 agcccgcgcg caccccggtg ccgtcgaccc cgacggtgag atcggtgacc agctcccgcg
     2041 cgatgtcgtc gacggtcctc gtgtccatgc cgggcgggtg cgcggccgcg acgtagtagc
     2101 ccgagcccat gatgatgttg agaccggtcg cccgcgccac ccgcgccagc gcgagcggat
     2161 cgcgcgcgag gcctcgattg gtcgggtcca cgaaggtctg cccgcccgcg tgcttgaaca
     2221 ggagggcctc gtcgcgggcc accgtctcgt ccagcagctg gaggttgtcg aggttggagc
     2281 tgaagtggtg ccggacccat ccgaggttgg ccaggctcac cggctgccgc gagagcccgc
     2341 gctccgcgcc ggtggcgggc tcgcggaaca tgacctcgaa gtcgatgagc aggtgctcgt
     2401 ggggcagggt gatgcccatc gcgtcccccg cgatggggcc gagcacggtc tgggcctggc
     2461 cggcgagcgc gcgcgtggcc attcagatcc cggcgtacca ctcgtagccg cggtcttccc
     2521 agtagccgcc gcggccgcgg gcgatgtgct cgaggcggtc caccagctcg accgccatca
     2581 cgtacttggc catcttgtag ccgagctgcc gctcgacgcg caggcggagg ggagcgccct
     2641 gcgccaccgg caggggctga tcgttcatct cgtaggcgag gatggtctgc tcgtggtagg
     2701 cgtccacgag gtcgatggat tcgtagtatg cctcgcccga gcccgccatg agatcggcgc
     2761 agcggaggac gacgtaacgg gcctcgggcc gcaccccggc tgcatcgagg agcgcgctca
     2821 tccgtacccc cgtccacttg gcgatcgcgc tccagccctc gacgcagtcg tgccgggtga
     2881 cctgggtgcg ggcgggcagg cggcgtagct ccctgagcga caggcgggcg ggccgctcga
     2941 cgaggccacg gacctcgagc cgccagtccg cgaaaccggc ggtggccgtg gcctggtagg
     3001 cgtcggtgcc gggatcgatg gtgccgttgc ccgggaacac cgcggagcgg gcggactccg
     3061 ggaactcgcg ggcgagagcc ccccgccgcg tgagggtccg ctgcacccgg tgggtgaccg
     3121 cctcgcccgc gtgcaggagc cgcgcgaccc acggcgtgcc cgagagacga tcgcagccgg
     3181 ccaggagcgg ggccgcggcc agggccaggc cgcgcagcac gaagcgccgc cggtcgagcg
     3241 gatgcggctc agccatccga ccctccgggg tgatgcacgc gcgcccagcc ggtgaggatc
     3301 gcgcgcagat tgttgaggac gccggtcgcc gccaccatga agacgtgcat gacggtgaac
     3361 ccgacgagcg cccaggtgag cgcgaagtgg acggtgcgcg cggactggcg gccgccgagg
     3421 agagcgggca ggagcggcag cgtcgcgtcg gcccacggcg acatggcgag cccggtgagc
     3481 accacgagcg ggcccaggac ggcgatcacg gtgacgtagg ccgccttctg cagcacgttg
     3541 taccgcggct cgccggccgg gaagcggaag cgcagatgat cgcgcaggga ggcgccgagc
     3601 ccggccaggt cgcgcccgtc cgggaggaga tcgcgcgtca ggtggcgact cagcagcgcg
     3661 tagacaccga aggtcagcgc gttcaacacg aagatccagg cgaagaagag gtgccagcgc
     3721 cggcccatgg cgagccactg cgatcccggc agggtcatcc aggccgggaa cccccgcgcg
     3781 acgcgctcgc cgtcctcggt ggaggcgccc aggagcccgg tggtgtcgag gcggtgaccc
     3841 cagagctcgg cgacgccgcg gatctcgtcg tcgccgatct cgatcgcgcg gagggcgaag
     3901 accgcgtgct ccgggtcgga gcgctcgccc cagtagagcg ccgggtgggc gttgaagatc
     3961 tgcagcccgc tgggcacgag cacgagcagg cagagcacgg tgatccagtg cgagaggcgg
     4021 gtggccgccg tgtggcggta gagccaggtg cgggacgcgg gcccgtcggt catgccgagg
     4081 accccgtggg tcaggcgggc gagggaggcg gcgtgccgcc gccgccacca ccgccgcgcc
     4141 gacgacggcc ccggcggcgg ccccggcgcc gcgggcgccc gggcgcgccg tccggcgggg
     4201 gcgcgcccgg ctcctggtcc gcctcgttcc cgggctcggg accggtgtcg gt
//
LOCUS       DATMCV010000921         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_316415, whole genome shotgun sequence.
ACCESSION   DATMCV010000921 DATMCV010000000
VERSION     DATMCV010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316415"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="VNU03_21020"
     CDS             <1..1953
                     /locus_tag="VNU03_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77016.1"
                     /translation="ARVAGFVAHPYAVTPYHGDYLEHFDLAEASRRAVDGPAEGAPAR
                     LIPRGPLAERLVPASKGGSAAKGDAMVVEEIDLQSLLAPYLVTAGGSFGPPWLKEGWF
                     HAYLLQASTMSDATARQAADQLFHRLVTGAYEGPAERADLARRLVGRLGAGCERAVVG
                     YSVRREPFNAEFSQGIENIAWDSQTGFDSEIFVRTAKLKDFPWNGWLRVGMATRPTAA
                     WNPVAGFTDPAGRLLWAALGDPAFLPGPYGAGPVPHRAVPASVVVGGAAGVEIPEDAL
                     LPEPGSGLLREVGRGKTAKAKVVYRLWGSAAHDNTRMSPADAVYPYIFAARWGSRRAQ
                     PGVEYDPAVDAATQTARQALAGFRVVKVDSEVRRYADITFTYVVPVVEVYLNAASPDP
                     QELVALAPPWGSVPWHVTVLMEEAVKRGVGAFSAGEARRRGVRWLDLARDAKTREALG
                     SILDGLARENHVPEPLRRLVAADEAQTRWAALRQFAQRRGHYLVTSGAYQLEKWSDAA
                     VVLQVFRDMTNPLGVGTYDRFAIPRRAYVARIAARGDRLEISPEIERLEKFLRQYRLV
                     REPLGSAADEDKVDVPSCRFVIVAADGRVAAAGISRDVQAHRVVVDLKGRLKPGTYTA
                     LVALVLGDNYVAPEVATAQFRVEGSP"
     gene            1975..2862
                     /locus_tag="VNU03_21025"
     CDS             1975..2862
                     /locus_tag="VNU03_21025"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77017.1"
                     /translation="MDAATLVVHLLNALLYASVMFLIAGGLSLIYGVMRIVNLAHGNL
                     YAFGAYVTAWAVGRALGTATEASLWMYALLPVGAVAAALLGAVLEPTLLRPLYRRAEE
                     YQLLITFGLLLILEDVMRLVWGPYPLQASALYEALGSLTIGDSIYPTYNLAVVVIGGV
                     AAAGLWAFVYRTHFGVVLRATSQNMRMASALGVNVNRVYVQAFTLGCFMAGLGGSVIV
                     PQQGAVLGMGVDALILAFVVVVIGGLGSLEGALIGALMVGLVREAGITFFPEIELAVL
                     YLMAAVVLLIRPAGILGRA"
     gene            2859..>3104
                     /locus_tag="VNU03_21030"
     CDS             2859..>3104
                     /locus_tag="VNU03_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77018.1"
                     /translation="MSPAAPPRRVLAWSGPVLAALIVLPFVVPPYQTVLLSYGLVLAI
                     AALGFNLLLGYTGLLSFGHSAYFGVGAYAVAFIVKYMK"
BASE COUNT          359 a         1113 c         1143 g          489 t
ORIGIN      
        1 gcgcgcgtcg ccgggttcgt ggcgcatccg tatgcggtca cgccgtacca cggcgactac
       61 ctcgagcact tcgacctggc cgaggcctcg cgccgcgcgg tggacggccc ggccgagggc
      121 gcgcccgcgc gtctgatccc ccgcggcccc ctcgcggagc gcctcgtgcc cgcgtcgaag
      181 ggcgggtccg cggccaaggg cgacgcgatg gtcgtcgagg agatcgacct ccagagcctg
      241 ctcgccccgt acctggtcac cgcgggcggc tccttcggcc cgccctggct caaggagggg
      301 tggttccacg cctacctgct ccaggcctcc acgatgagcg atgcgacggc ccgccaggcg
      361 gccgatcagc tcttccaccg cctcgtcacc ggcgcctacg agggtcccgc cgagcgcgcc
      421 gacctcgcgc ggcggctggt cggccgtttg ggggcggggt gcgagcgcgc ggtcgtgggc
      481 tactcggtcc ggcgcgagcc gttcaacgcc gagttctccc agggcatcga gaatatcgcg
      541 tgggactcgc agaccggctt cgactccgag atcttcgtgc gcaccgccaa gctcaaggac
      601 ttcccgtgga acggctggtt gcgcgtgggc atggccaccc ggcccaccgc ggcgtggaac
      661 ccggtcgcgg gcttcaccga cccggccggg cgactgctct gggccgcgct cggcgacccc
      721 gcgttcctgc ccgggcccta cggcgcgggc ccggtcccgc atcgggcggt gccggcctcg
      781 gtggtggtgg gcggcgcggc gggcgtggag attcccgagg acgcgctgct cccggagccg
      841 ggtagcggtc tcctccgcga ggtcggccga ggcaagaccg cgaaggccaa ggtggtctac
      901 cggctgtggg gatcggccgc ccacgacaac acgcggatga gccccgcgga tgcggtctac
      961 ccgtacatct tcgcggcgcg ctggggctcg cggcgcgccc agcccggcgt ggagtacgac
     1021 ccggcggtcg acgcggccac ccagaccgcg cgccaggcgc tcgccggctt ccgggtggtg
     1081 aaggtcgaca gcgaggtgcg gcgctacgcc gacatcacct tcacctacgt ggtgccggtg
     1141 gtcgaggtct acctgaacgc ggcctcgccc gatccccagg agctggtcgc gctggccccg
     1201 ccctggggct cggtgccgtg gcacgtgacg gtgctgatgg aggaggcggt caagcgcggg
     1261 gtgggcgcct tctccgcggg cgaggcgcgc cgccgcgggg tgcgctggct ggacctcgcg
     1321 cgcgacgcga agacgcgcga ggcgctcggc tccatcctgg acgggctcgc gcgcgagaac
     1381 cacgtgccgg agccgctccg ccgcctagtg gccgccgacg aggcgcagac gcggtgggcc
     1441 gccctccggc agttcgccca gcggcgcggg cactacctgg tgacgagcgg ggcctaccag
     1501 ctcgagaagt ggagcgacgc ggcggtggtg ctccaggtct tccgcgacat gaccaacccg
     1561 ctcggggtgg ggacctacga ccgcttcgcg atcccgcggc gtgcctacgt cgcgcgcatc
     1621 gccgcgcgtg gcgaccggct cgagatctct cccgagatcg agcggctcga gaagttcctc
     1681 cgccagtacc ggctcgtccg ggagccgctc gggtcggcag ccgacgagga caaggtcgac
     1741 gtgccgtcgt gccggttcgt gatcgtggcc gccgacgggc gcgtcgccgc cgccggtatc
     1801 tcccgcgacg tgcaggccca ccgggtggtg gtcgacctga aggggcggct caagccgggg
     1861 acgtacaccg ccctggtggc cctcgtcctg ggcgacaact acgtggcccc cgaggtcgcg
     1921 accgcgcagt tccgggttga aggctccccc tgaattgagg catcttatcc gctcatggat
     1981 gccgcgaccc tcgtagtcca tctcctcaac gcgctcctct acgcgtcggt gatgttcctg
     2041 atcgcgggcg ggttgagcct gatctacggg gtcatgcgca tcgtgaacct ggcgcacggc
     2101 aacctctacg cgttcggcgc ctacgtgacc gcgtgggcgg tggggcgcgc gctcggcacc
     2161 gccaccgagg cgagcctctg gatgtacgcg ctgctgccgg tgggcgcggt ggccgccgcg
     2221 ctgctgggcg cggtgctgga gccgacgctg ctgcgcccgc tctaccggcg ggccgaggag
     2281 taccagctgc tcatcacctt cggcctcctc ctgatcctgg aagacgtcat gcggctggtg
     2341 tggggaccgt acccgctcca ggccagcgcg ctctacgagg cgctcggcag cctcaccatc
     2401 ggcgactcga tctacccgac gtacaacctg gcggtggtcg tcatcggcgg cgtcgcggcg
     2461 gcggggctgt gggccttcgt gtaccgcacc cacttcggag tggtgctgcg ggccacgtcg
     2521 cagaacatgc ggatggcctc cgccctcggg gtcaacgtca atcgcgtcta cgtgcaggcc
     2581 ttcaccctcg gctgcttcat ggccggcctg ggcggctcgg tgatcgtgcc ccagcagggg
     2641 gcggtgctcg gcatgggcgt cgacgcgctg atcctggcct tcgtggtggt ggtcatcggg
     2701 ggcctcggca gcctcgaggg cgcgctgatc ggggccctca tggtggggct cgtgcgcgag
     2761 gcggggatca cgttctttcc cgagatcgag ctggccgtgc tctacctgat ggcggcggtc
     2821 gtgctcctca tccggccggc cgggatcctg gggcgcgcgt gagcccggct gcgccgccgc
     2881 gccgggtgct cgcctggtcg gggccggtcc tggccgcgct gatcgtgctg ccgttcgtgg
     2941 tgccgcccta ccagacggtg ctgctctcct acgggctcgt gctcgccatc gccgcgctcg
     3001 gcttcaacct cctcctcggc tacaccggcc tgctgtcgtt cggccactcc gcgtacttcg
     3061 gggtgggggc ctacgcggtg gccttcatcg tgaagtacat gaag
//
LOCUS       DATMCV010000922         3754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_388825, whole genome shotgun sequence.
ACCESSION   DATMCV010000922 DATMCV010000000
VERSION     DATMCV010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388825"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VNU03_21035"
     CDS             <1..702
                     /locus_tag="VNU03_21035"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HWP77019.1"
                     /translation="ASTSRTSHVGRLADYAEMAAGAGLVGMLWANCVHGLNVAPWGGA
                     ARRLGTNPHAVGIPGAGGPAMVLDFATSVVAEGKLRVKRNRKQPAPPGWFVDSKGRPE
                     TDPEIFYGDPPGALLTAGDHKGYGLSLAVEILGGILSGTGPAGPPPGVFANGTLMICL
                     DVERFLPLPDFHRQVAALFGWVKATPLAQGATEILIPGEPEARLEAERRANGIAIEDQ
                     TWSQIETTAAELGVG"
     gene            complement(716..1540)
                     /locus_tag="VNU03_21040"
     CDS             complement(716..1540)
                     /locus_tag="VNU03_21040"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP77020.1"
                     /translation="MRRVALLAVMLAGLGATPLRAQSPDLPIFDTHIHYSAPDWAEYP
                     PDRILGILQKAGIKRALVSSTPDDGTLTLYQKDPKRVVPILRPYRTRDDIGHWWQDPS
                     VLAYVQERLAKNKGVHKGIGEFHLFGGQTGTFVVKRITELAVQENIYLHAHSDELAIV
                     ELFTLEPRLRVIWAHAGMSAGPQAVGALLDRYPNLWVDLAIRNGDVAPGGTLDPGWRA
                     VFLRHPDRFLAGTDTWMTSRWEQLPGSVTEVHGYLGQLPRDVAEKIAFRNAERLFP"
     gene            1609..1974
                     /locus_tag="VNU03_21045"
     CDS             1609..1974
                     /locus_tag="VNU03_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77021.1"
                     /translation="MNTVRTVLTLGLLVLATAAPAAAQGLPGGRRPDAIDQARGLATQ
                     PFQTPATPDRPAERWISPRRVYLPELGRDVQVPGHYERDVNGQRVERPPLIVTTPEGG
                     SPTILPGGERPPVEQRTGP"
     gene            1974..2192
                     /locus_tag="VNU03_21050"
     CDS             1974..2192
                     /locus_tag="VNU03_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77022.1"
                     /translation="MLEAVGMAEINKIFAVTDALGVHREALVIPLGTGTGRVRKLPSG
                     KFEIIVDKNVPIDEWLEGLPALIQAAMA"
     gene            2189..3493
                     /locus_tag="VNU03_21055"
     CDS             2189..3493
                     /locus_tag="VNU03_21055"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWP77023.1"
                     /translation="MSPRRRGEIGLLPLVAILFFNVSGGPYGVEDAVGSLGPGLALLL
                     LVVTPLVWSLPVSLAMAELASALPEEGGYVVWVQRAFGRFWGFQAGWWSWINSFVDVA
                     VYPALFADYLAFWRPGMSTLERWAVVLAFVWILTAVNLVGVRITGWTAVALGVVSLVP
                     VVVLTVVAATRLEAVPWRPFAAEDGGLLSGLGLGLAVMMWNYSGWDTPTTTLGETHEP
                     GPSFRRAVWVALPLITLAYVLPVAAGLSAAGDWAAWDTGHWPAVALHVGGAWLAHLVT
                     LGAVVAAAGLFLSLLLTNSRLPYALARDGQLPAALGRLHPRFGTPWAAVVLSSVGYSV
                     CAFWSFKELIVLNIWLYSIALLLEMAAFVALRLREPALPRPWRVGGGATGMWLVASLP
                     ALCCLLAMATAGWANTTVGVLAALTGPLAYWLWRAPARRGSA"
     gene            complement(3490..>3754)
                     /locus_tag="VNU03_21060"
     CDS             complement(3490..>3754)
                     /locus_tag="VNU03_21060"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWP77024.1"
                     /translation="VAAFYGQAAAVDALLSAGADVNARSTDGSLPIHDAAHGGSVPTA
                     ETLLRHGADLNDRDHRYDATPLAVARYFGKSAMAEFLAGRGAA"
BASE COUNT          447 a         1356 c         1389 g          562 t
ORIGIN      
        1 gcgtccacct cgcgcaccag ccacgtgggg cggctcgccg actacgccga gatggcggcc
       61 ggcgccgggc tcgtcggcat gctctgggcc aactgcgtgc acgggctcaa cgtggcgccg
      121 tggggtggcg ccgcgcggcg gctcggcacc aacccgcacg cggtcggcat ccccggcgcg
      181 ggcgggcccg cgatggtgct cgacttcgcc accagcgtgg tggccgaggg caagctgcgg
      241 gtgaagcgca accgcaagca gcccgcgccg cccggctggt tcgtggacag caagggccgg
      301 cccgagaccg atcccgagat cttctacggc gatccgcccg gcgcgctgct caccgcgggc
      361 gaccacaagg gctacggcct ctcgctcgcg gtggagatcc tgggcggcat cctctccggc
      421 accggccccg cgggcccgcc gcccggcgtc ttcgccaacg ggacgctgat gatctgcctc
      481 gacgtggagc gcttcctgcc gctgcccgac ttccaccggc aggtggccgc gctcttcggc
      541 tgggtgaagg cgacgccgct cgcccagggc gcgaccgaga tcctgatccc cggcgagccg
      601 gaggcacgcc tcgaggccga gcgccgcgcg aacggcatcg ccatcgagga tcagacgtgg
      661 agccagatcg agacgaccgc ggcggagctg ggggtcggct agaccccgac ccggatcagg
      721 ggaacaggcg ctccgcgttc cggaaggcga tcttctcggc cacgtcgcgc gggagctgac
      781 cgagatagcc gtggacctcg gtcaccgagc cggggagctg ctcccatcga gaagtcatcc
      841 aggtgtcggt gccggccagg aatcgatccg gatggcggag gaacaccgcg cgccagcccg
      901 ggtcgagtgt gccgccgggc gccacgtcgc cgttccggat ggccaggtcc acccacagat
      961 tcggatagcg gtcgagcagc gcccccaccg cctgcggccc cgcgctcatg cccgcgtggg
     1021 cccagatcac ccgcagccgc ggctcgagcg tgaacagctc gacgatggcc agctcgtccg
     1081 agtgcgcgtg gaggtagatg ttctcctgca ccgccagctc ggtgatgcgc ttgaccacga
     1141 aggtgccggt ctggccgccg aagagatgga actccccgat gcccttgtgc acgcccttgt
     1201 tcttcgcgag gcgctcctgc acgtaggcca gcaccgacgg gtcctgccac cagtggccga
     1261 tgtcgtcgcg cgtgcgatac ggccggagga tcgggacgac gcgcttgggg tccttctggt
     1321 aaagggtgag ggtaccgtcg tcgggggtgc tcgacacgag cgcgcgcttg atgcccgcct
     1381 tctggaggat gcccaggatg cggtcgggcg ggtactcggc ccagtcgggc gcgctgtagt
     1441 ggatgtgggt gtcgaagatc gggaggtccg ggctctgggc gcggagcggg gtcgcgccga
     1501 gaccggccag catcaccgcg aggagggcga cacgccgcac ggcgacgagc ctagcacggc
     1561 cacgtcgcct tgcgctcccc gttccggtcc gctacgctgg gcgacgtcat gaacacggta
     1621 cgaacggttc tcacgctcgg gttgctcgtt cttgctaccg ccgcgccggc cgcggcccag
     1681 ggcctgccgg gcggccgccg gcctgacgcc atcgaccagg cgcgggggct cgcgacccag
     1741 cccttccaga cccccgcgac gccggaccgc ccggccgagc gctggatctc gccgcggcgc
     1801 gtgtatttgc ccgagctggg gcgcgacgtc caggttcccg gccactacga gcgcgacgtc
     1861 aacgggcagc gggtcgagcg gcctccgctc atcgtgacca cgccggaggg cgggagcccg
     1921 acgatcctgc cgggcggcga gcgcccgccg gtcgagcagc ggaccggccc ctgatgctgg
     1981 aggcggtggg catggcggag atcaacaaga tcttcgcggt caccgacgcg ctcggcgtcc
     2041 accgcgaggc cctcgtcatc ccgctcggca ccggcaccgg ccgcgtgcgc aagctgccga
     2101 gcggcaagtt cgagatcatc gtcgacaaga acgtcccgat cgacgagtgg ctcgagggac
     2161 tgcccgcgct gatccaagcc gccatggcgt gagtccccgc cgacgcgggg agatcgggct
     2221 ccttcccctc gtcgccatcc tcttcttcaa cgtgagcggc gggccgtacg gcgtcgagga
     2281 cgcggtcggc tcgctcggtc ccggcttggc cctcctgctg ctggtcgtca ccccgctggt
     2341 gtggagcctg cccgtctcgc tcgccatggc cgagctggcc tcggcgctgc ccgaggaggg
     2401 cggctacgtg gtctgggtgc agcgcgcctt cggccgcttc tgggggttcc aggccgggtg
     2461 gtggagctgg atcaactcct tcgtggacgt ggcggtctac ccggcgctct tcgccgacta
     2521 cctggccttc tggcggccgg gcatgtccac cctcgagcgc tgggcggtcg tcctcgcctt
     2581 cgtctggatc ctgaccgcgg tgaacctggt cggcgtgcgc atcaccggct ggaccgcggt
     2641 cgcgctgggc gtggtctcgc tggtgccggt cgtcgtgctc acggtggtgg cggcgacccg
     2701 gctcgaggcg gtgccgtggc ggcccttcgc ggccgaggac ggtggtctcc tctcgggcct
     2761 cggcctgggc ctcgcggtca tgatgtggaa ctactcgggc tgggacacgc ccaccaccac
     2821 gctcggcgag acgcacgagc cggggcccag cttccgtcgc gcggtgtggg tcgcgctgcc
     2881 gctgatcacg ctggcctacg tgctgcccgt cgcggccggc ctctccgcgg ccggcgactg
     2941 ggccgcgtgg gacaccgggc actggccggc ggtcgcgctg cacgtcggcg gcgcgtggct
     3001 cgcgcacctc gtgacgctcg gcgcggtggt ggcggcggcc gggctcttcc tctcgctcct
     3061 gctcacgaac tcgcgcctgc cctacgcgct ggcgcgcgac gggcaattgc cggccgcgct
     3121 tggccgcctg cacccgcgct tcgggacgcc gtgggccgcg gtggtcctct cgagcgtggg
     3181 ctacagcgtc tgcgccttct ggagcttcaa ggagctgatc gtgctgaaca tctggctgta
     3241 ctcgatcgcg ctgctgctcg agatggccgc gttcgtggcg ctgcgcctgc gcgagcccgc
     3301 gctgccgcgg ccgtggcgcg tgggcggcgg cgcgaccggg atgtggctcg tggcctcact
     3361 gcccgcgctc tgctgcctgc tcgcgatggc caccgcgggg tgggccaaca cgaccgtcgg
     3421 cgtcctcgcc gcgctgaccg gtccgctggc ctactggctc tggcgcgcgc cggcgcgccg
     3481 cggatcggcc taggcggcgc cgcgcccggc gaggaactcg gccatcgcgc tctttccgaa
     3541 gtagcgcgcc accgccagcg gggtggcgtc gtagcggtga tcccggtcgt tcaggtcggc
     3601 gccgtgacgc agcagcgtct ccgcggtggg cacgctcccg ccgtgggccg catcgtgtat
     3661 cggcagcgag ccgtcggtgg agcgggcgtt gacgtcggcc ccggccgaga ggagcgcgtc
     3721 caccgcggcc gcctggccgt agaacgcggc cacg
//
LOCUS       DATMCV010000923         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_713581, whole genome shotgun sequence.
ACCESSION   DATMCV010000923 DATMCV010000000
VERSION     DATMCV010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713581"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VNU03_21065"
     CDS             complement(<1..447)
                     /locus_tag="VNU03_21065"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP77025.1"
                     /translation="MTTPSPTRALASDIDPVRFEVIRNALLAITEEMGATLRRAAYST
                     NIKTRGDFSCAFFDHDLRTIAQAFAQPSHLGSLAHIVPKAIATYGAERLAPGDGILIN
                     DPYLGGVHLNDITLITPVFHEGALFGYVANIAHHVDVGGGAPGSIGV"
     gene            complement(444..860)
                     /gene="hpxZ"
                     /locus_tag="VNU03_21070"
     CDS             complement(444..860)
                     /gene="hpxZ"
                     /locus_tag="VNU03_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HWP77026.1"
                     /translation="MTGDPELAPNLPEIVAEVRALFERYEQALIDKDVDVLDTTFWDS
                     PHTIRYALNENGYGFGEIHAHRVARPPGPGLKERRIRLEILTLGRDLATVNLEFKVRG
                     RDLVGRQSQTWVRFPALGWKVIAAHVSTMNEAPLWP"
     gene            complement(857..2023)
                     /locus_tag="VNU03_21075"
     CDS             complement(857..2023)
                     /locus_tag="VNU03_21075"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP77027.1"
                     /translation="MTSPFVPGPVLRIAGAPGGPLAGLTFAAKDLFDVAGHPTGGGNP
                     DWGRAHPVPTRHAWAVQRLLDAGARLVGKTVTDEVSLGILGENPFDGTPLNPMAPDRV
                     PGGSSSGSASAVAQGLCDTALGTDTGGSVRVPASFCGLHGIRPTHGRLDLTGMMRQAP
                     GSDTTGWFARDASTFARVSEVLLGEPAAAELPARLVIAADAFGLADDETAAALQPMVG
                     RLSELVKDVREEMLAPPGLSVWARAQRMLQSFEAWTTFKDWIDRENPRMAWSVARNLA
                     LAATISEPERQWATLTRAEARARLSWLLPPGTILCLPTTPFPAPRKGLPLATLDPLRA
                     RITCLAAHGGLTGVPQVTLPGAHVNGLPVGLSILAARGADATLVAVAKAMEAGE"
BASE COUNT          275 a          689 c          793 g          267 t
ORIGIN      
        1 cactccgatg ctgcccgggg cgccgccgcc cacgtccacg tggtgcgcga tgttcgccac
       61 gtagccgaag agcgccccct cgtggaacac gggggtgatc agggtgatgt cgttgaggtg
      121 cacgcccccc aaatacgggt cgttgatgag gatgccgtcg ccgggggcca gccgctcggc
      181 cccgtaggtc gcgatggcct tcggcacgat gtgggcgagc gagcccaggt gcgagggctg
      241 ggcgaaggcc tgggcgatgg tgcgcaggtc gtgatcgaag aacgcgcagg agaagtcccc
      301 gcgggtcttg atgttggtcg agtaggcggc gcgccggagc gtcgccccca tctcctcggt
      361 gatggcgagg agcgcgttgc ggatgacctc gaaccggacg gggtcgatgt cggaggcgag
      421 ggcgcgggtc ggggacggag tcgtcatggc cagagtgggg cctcgttcat ggtggagacg
      481 tgggcggcga tcaccttcca gccgagcgcg gggaagcgca cccaggtctg gctctgccgg
      541 ccgacgaggt cgcggccccg caccttgaac tcgaggttca cggtggcgag gtcgcggccg
      601 agggtgagga tctcgaggcg gatgcgccgc tccttgaggc cgggtccggg cggccgggcc
      661 acccggtggg cgtggatctc gccgaagccg tagccgttct cgttgagcgc gtagcggatg
      721 gtgtgggggc tgtcccagaa ggtcgtgtcg agcacgtcga cgtccttgtc gatcagggcc
      781 tgctcgtagc gctcgaacag cgcgcgcacc tcggccacga tctccggcag attgggggcc
      841 agctccgggt cgccggtcat tcgcccgctt ccatggcctt cgcgaccgcc acgagcgtgg
      901 cgtcggcgcc gcgcgcggcc aggatggaga gccccactgg cagcccgttc acgtgagcgc
      961 cgggcagcgt cacctgcggc accccggtga gcccgccatg cgcggccagg caggtgatgc
     1021 gcgcgcgcag cgggtcgagg gtcgcgagcg gcaggccctt gcggggcgcc ggaaacggcg
     1081 tcgtcggcag gcacaggatc gtgcccggcg gcaggagcca ggagagccgc gcccgggcct
     1141 cggcgcgggt cagcgtggcc cactgccgct ccggctccga gatcgtggcc gcgagcgcca
     1201 ggttgcgcgc cacgctccac gccatgcgcg ggttctcgcg atcgatccag tccttgaagg
     1261 tcgtccaggc ctcgaacgac tgcagcatgc gctgcgcgcg cgcccagacc gacaggccgg
     1321 gcggggccag catctcctcg cgcacgtcct tcaccagctc cgagagccgg cccaccatcg
     1381 gctggagcgc ggccgcggtc tcgtcatccg cgaggccgaa cgcgtcggcc gcgatcacga
     1441 ggcgggccgg cagctccgcc gcggcgggct cgcccagcag cacctccgag acgcgcgcga
     1501 aggtggacgc gtcgcgggcg aaccacccgg tggtgtcgga gcccggcgcc tgccgcatca
     1561 tgccggtcag atcgagccgg ccgtgggtcg gccggatgcc gtggaggccg cagaagctcg
     1621 ccggcacgcg caccgatccg ccggtgtcgg tgccgagcgc ggtgtcgcac agcccctggg
     1681 ccaccgccga ggccgagccc gacgaggagc cgcccggcac gcggtcgggc gccatcgggt
     1741 tgagcggagt gccgtcgaag ggattctcgc cgaggatgcc gagcgagacc tcgtcggtga
     1801 cggtcttgcc gaccagcctt gcgcccgcgt cgagcaagcg ctggacggcc caggcgtggc
     1861 gcgtcgggac gggatgcgcg cgcccccagt ccgggttgcc gccaccggtc gggtggccgg
     1921 ccacgtcgaa cagatccttg gccgcgaagg tcaggccggc gagggggccg cccggagcgc
     1981 cggcgatgcg cagcaccggg ccaggcacga agggactggt catg
//
LOCUS       DATMCV010000924         5743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_516827, whole genome shotgun sequence.
ACCESSION   DATMCV010000924 DATMCV010000000
VERSION     DATMCV010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516827"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..764
                     /locus_tag="VNU03_21080"
     CDS             78..764
                     /locus_tag="VNU03_21080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP77028.1"
                     /translation="MTRAAMGVLHDPGLERLLAALHARSDEQVAAIASYQAQRAGAPP
                     TADELKTFRRDKLVALDRDKAQLCYQLCRASQARMIVEAGTSYGVSTLYLAAAVRDNV
                     RAAGGHGVVIGTEYEPTKASVARAHFDQAGLSGFIDLREGDLRETLKQVEGPIDFMLV
                     DIWIPMARPALELVAPHLRPGAIVICDNTELYRTEYADYLAFLEDPAHRFRTMTLPFD
                     GGLELSVRVP"
     gene            866..1972
                     /locus_tag="VNU03_21085"
     CDS             866..1972
                     /locus_tag="VNU03_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP77029.1"
                     /translation="MVTDVPIISADSHITEPPDTYTARIDVRFKDRAPRLVHDAKRGD
                     LFIIDGLDKPIPMGLIAAAGKPAEELAMFGARFEDLHRGGWDPEARLADQARDGVSAE
                     VIYPTVGMMICNHPDFDYKQACFDAYNLWIAEYCAAHPDRLLGVGQTAMRSVEDGIRD
                     LQRIRELGLRGVMLPGNPAVADYHDPIYAPFYEAAIDLGLPLSFHILTSQQDTFKVRG
                     PKLNAFMSIIRGCQDLVGTFVLGGVFERHPRLRLVCVEADAGWVPHFMYRMDHAYDRH
                     RYWLPSGTLTKMPSEYFREHVYTTFQDDWVAFQVKDLCNIRRLLWANDFPHSDSTWPR
                     SQELLAKHASHLSPAELRLILHDNVAELYGLAGA"
     gene            1976..3688
                     /locus_tag="VNU03_21090"
     CDS             1976..3688
                     /locus_tag="VNU03_21090"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HWP77030.1"
                     /translation="MDFDLVTRGGTIVDGTGRPGVRGDVGIRDGRIVAVGEAKGAAAQ
                     TVDAGGLVVAPGFIDVHTHYDAQVMWDRMLTISPWHGVTTVVMGNCGFGVAPTRPDHR
                     GLIMRTLEKVEGMSLAALEAGLGEKWPFETFPEYLDAVERRGTALNVAVLLGHTPLRL
                     YVMGEEATERPATDDEIARMRALVREAIDAGAIGFATSKASTHIGYAGKPVPSRAAQF
                     AEIEALAGALGDAGRGIVQATVGRDLFFKEFESLARSSGRPVTWTALLAGMMGPGSHR
                     ELLEKSRALIKQGLQVVPQVTCRTLNFEFQFKEPFIFESMSVFKPISVADFAGKVRLY
                     GDPDFRRAFKESFDRPKKGAVFAGLSWARTWISWYPPDPSLEERTVDEVAAERSVHPI
                     DLALDLALATNLDARFRMAVFNHDEAEVAELLAEPDAVLGLSDAGAHASQLCDACFST
                     YLLSRWVREKQAIALPEAIRMLTSRPAEVFGITDRGRLAPGLAADVVVFDPATVGASK
                     LRRVHDLPAGADRLVADASGIEAVIVNGTVIRRGGHDAVAADGALPGALLRNGSARAT
                     GRAR"
     gene            3696..4733
                     /locus_tag="VNU03_21095"
     CDS             3696..4733
                     /locus_tag="VNU03_21095"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HWP77031.1"
                     /translation="MIPVIDLEPYLAKRPGAREATAAELGRALQDIGFFVIVNHGIPS
                     SLIDATFAEARRFHAQPMDAKLALRMNEHNNGYMMLGRYAVWTSDVNANDKPDLNEAF
                     FVKRERPPDDPLVRAGRRFAGPNQWPARLPGFREAVLAYTDAVDALGRQLLPLCATAL
                     DLPPDTFDVAFAESQFSFRLTHYPPVASEPNQFGIAPHTDANFMTFLAQSEVPGLQVR
                     LPDDTWADVPYVPGSFAVNSGDMLKRWTNDRFKSTPHRALPPVGRPRYAIPYFMGPHL
                     DTEIACLPTCRGPENPPRHPPITYAAYLDWWYDANYNASDQRDAAAARQRDVVASDQR
                     DVAAARQRDVA"
     gene            4760..>5743
                     /locus_tag="VNU03_21100"
     CDS             4760..>5743
                     /locus_tag="VNU03_21100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77032.1"
                     /translation="MKVGIALNMLTQEGRPDAAVLGDHLALGDLAEPLGFDSLFALEH
                     HFTGYAMSPSPTQLLSYFAGRTKRVTLGTAVIVLPWHDPVRIAEEIALLDILSGGRCL
                     LGFGRGAASVEYAGFRIPMEEARARFVETAQIVVRALSQDVFEWDGEFFKIPRTSIRP
                     RPISHPERRIYASSVSPESAEIMAKLGFGVLVIMQNEWPKAAEDIQRYREIAQSVGHA
                     PRPPIILTNVSVAESRTEAHERAVKYLSRKFDSIDTHYRFSDGHLGSVKGYESYGATA
                     KTYAKMKDANFRQKAADFYVKIQVVGTPDECLQQLSELRRLTGLDHLVTEFA"
BASE COUNT          876 a         2055 c         1957 g          855 t
ORIGIN      
        1 tcgtgccgcg agaaccggca gtatccgcgc gtgatcctcg agttctggac tcccgcctat
       61 caccgctggc tgcagggatg acccgcgcgg cgatgggcgt ccttcacgac cccgggctgg
      121 agcgcttgct ggccgccttg cacgcgcgga gtgacgagca ggtcgccgcg atcgcgagct
      181 accaggcgca gcgggccggg gccccgccca ccgcggacga gctcaagacc tttcgccgcg
      241 acaagctggt cgccctcgat cgcgacaagg cgcagctctg ctatcagctc tgccgggcga
      301 gccaggcccg catgatcgtc gaggccggca cctcctacgg ggtctcgacg ctctatctcg
      361 ccgcggcggt gcgcgacaac gtgcgtgccg ccggcgggca cggcgtggtg atcggcaccg
      421 agtacgagcc gacgaaggcg tcggtcgcgc gagcgcattt cgaccaggcg ggcctgagcg
      481 gcttcatcga tctccgggaa ggcgatctcc gcgagacgct caagcaggtc gagggcccga
      541 tcgacttcat gctggtggat atctggatcc ccatggcgcg gccggcgctg gagctggtcg
      601 ccccccacct gaggcccggc gccatcgtca tctgcgacaa caccgagttg taccggaccg
      661 agtacgccga ctacctcgca ttcctcgaag atcccgccca ccgattccgc acgatgacat
      721 tgcccttcga tggcggcctg gagctatcgg ttcgcgtgcc gtgacgcccg cggctttaca
      781 ccggcggtaa tcttcgctac gctggcccgg cggtcggggc gcggccccgg gacattcccg
      841 ctgatcgtct tcgaggagac gcgccatggt caccgacgtc ccgatcatct ccgccgactc
      901 ccacatcacc gagccgcccg acacctacac cgcccggatc gacgtgcgct tcaaggatcg
      961 cgccccccgc ctcgtgcacg acgccaagcg cggcgatctc ttcatcatcg acgggctcga
     1021 caagcccatc ccgatgggcc tgatcgccgc cgccggcaag ccggcggagg agctcgcgat
     1081 gttcggggcc cgcttcgagg acctgcatcg gggcggctgg gaccccgagg cccgactcgc
     1141 ggaccaggcg cgcgacgggg tcagcgccga ggtgatctac ccgaccgtcg gcatgatgat
     1201 ctgcaaccac cccgacttcg attacaagca ggcgtgcttc gacgcctaca acctgtggat
     1261 cgccgagtac tgcgccgcgc atcccgaccg gttgctgggc gtcgggcaga ccgcgatgcg
     1321 ctcggtggag gacggtatcc gcgatctcca gcgcatccgt gagctcggcc tgcgcggagt
     1381 catgctgccg gggaacccgg cggtggccga ctaccacgac ccgatctatg cgccgttcta
     1441 cgaggcggcg atcgacctcg ggctgccgct ctcctttcac atcctcacga gccagcagga
     1501 cacgttcaag gtccgcgggc ccaagctcaa cgccttcatg tcgatcatcc gcggctgcca
     1561 ggatctggtc gggacgttcg tgctcggcgg ggtcttcgag cggcatccgc gtctgcgcct
     1621 ggtctgcgtc gaggccgacg ccgggtgggt gccgcacttc atgtaccgca tggaccacgc
     1681 ctacgaccgg caccgttact ggctgccgtc ggggacgctc accaagatgc cgagcgagta
     1741 cttccgcgag cacgtctaca ccaccttcca ggacgactgg gtcgccttcc aggtgaagga
     1801 cctctgcaac atccgccggc tcctctgggc caacgacttc ccccacagcg actcgacgtg
     1861 gccgcgctcg caggagctcc tcgccaagca cgcctcccac ctgagcccgg cggagctgcg
     1921 cctgatcctc cacgacaacg tggccgagct ctacggcctg gcgggagcct gagcgatgga
     1981 cttcgatctc gtcacacggg gcggcacgat cgtcgacggc accggacgtc cgggggtgcg
     2041 cggcgacgtg gggatccggg acggccggat cgtcgcggtg ggcgaggcga agggcgccgc
     2101 cgcgcagacg gtcgacgcgg gcgggctcgt ggtcgcgccc ggcttcatcg acgtccacac
     2161 ccactacgac gcgcaggtga tgtgggaccg gatgctcacg atctcgccct ggcacggggt
     2221 gaccacggtc gtgatgggca actgcggctt cggcgtggcg ccgacgcggc cggatcatcg
     2281 cgggctcatc atgcgcacgc tcgagaaggt cgagggcatg agcctcgccg cgctcgaggc
     2341 gggcctcggc gagaagtggc cgttcgagac cttccccgag tatctcgacg cggtcgagcg
     2401 gcgcggcacc gcgctgaacg tggcggtgct cctgggccac accccgctgc ggctctacgt
     2461 catgggcgag gaggcgaccg agcggccggc gacggatgac gagatcgcgc ggatgcgggc
     2521 gctggtgcgc gaggcgatcg acgcgggcgc gatcggcttc gcgacctcga aggcgtccac
     2581 ccacatcggc tacgccggca agccggtgcc gagccgggcc gcccagttcg ccgagatcga
     2641 ggcgctggcc ggggcgctgg gcgacgcggg acggggcatc gtccaggcca ccgtcggccg
     2701 cgacctgttc ttcaaggagt tcgagagcct cgcccggtcc agcggccggc cggtgacgtg
     2761 gaccgcgctc ctggccggca tgatggggcc cggctcccac cgtgagctgc tcgagaagtc
     2821 ccgcgcgctg atcaagcagg gcctgcaggt ggtgccgcag gtcacctgcc ggacgctgaa
     2881 cttcgagttc cagttcaagg agccgttcat cttcgagagc atgtcggtgt tcaagccgat
     2941 ctcggtcgcc gacttcgcgg gcaaggtccg gctctacgga gaccccgact tcaggcgcgc
     3001 gttcaaggag agcttcgacc ggccgaagaa gggcgcggta ttcgccggac tgagctgggc
     3061 gcggacgtgg atctcctggt acccgccgga tccctcgctc gaggagcgca cggtcgacga
     3121 ggtggcggcg gagcgcagcg tgcatccgat cgacctcgcg ctcgacctgg cgctggcgac
     3181 caacctggac gcccggttcc ggatggcggt gttcaaccac gacgaggccg aggtggccga
     3241 gctgctcgcg gagcccgacg cggtgctggg gctgtccgac gcgggcgccc acgcgagcca
     3301 gctctgcgac gcctgcttct cgacctacct gctcagccga tgggtgcggg agaagcaggc
     3361 gatcgcgctg ccggaggcga tccgcatgct cacctcgcgg ccggcggagg tgttcggcat
     3421 caccgaccgg gggcgcctcg ctcccggcct cgcggccgat gtcgtggtct tcgacccggc
     3481 caccgtgggg gccagcaagc tgcggcgcgt gcacgacctg ccggcggggg ccgatcgact
     3541 ggtggccgac gccagcggca tcgaggcggt gatcgtcaac ggcacggtga tccgtcgcgg
     3601 cgggcacgac gcggtcgcgg cggacggcgc cctgccggga gccctgctgc gcaacggcag
     3661 cgcgagggcg accggccgag ctcgctgagc tggtgatgat ccccgtcatc gacctcgagc
     3721 cgtacctggc gaagcgcccg ggcgcccggg aggccaccgc cgccgagctg ggccgcgccc
     3781 tgcaggacat cggcttcttc gtgatcgtga accacggcat cccgtcgtcc ctcatcgacg
     3841 cgacgttcgc cgaggcccgg cgctttcacg cgcagccgat ggacgccaag ctcgccctgc
     3901 gcatgaacga gcacaacaac gggtacatga tgctcgggcg gtacgcggtg tggacctcag
     3961 acgtgaacgc caacgacaag cccgacctca acgaggcgtt cttcgtcaag cgcgagcgcc
     4021 cgccggacga ccccctcgtg cgcgcgggac ggcgcttcgc cgggccgaac cagtggccgg
     4081 cgcgcctgcc cggctttcgc gaagcggtgc tcgcctatac cgacgcggtg gacgctctgg
     4141 ggcggcagct cctgcccctg tgcgcgacgg ctctcgatct gccgccggac accttcgacg
     4201 tcgccttcgc cgagagccag ttcagcttcc gtctcaccca ctacccgccg gtggcgtccg
     4261 agccgaacca gttcggcatc gcgccgcaca ccgacgccaa cttcatgacg tttctcgccc
     4321 agagcgaggt gcccgggctc caggtgcgcc tgccggacga cacgtgggcc gacgtgccgt
     4381 acgtgcccgg ctccttcgcg gtgaactccg gcgacatgct caagcggtgg accaacgacc
     4441 ggttcaagtc caccccgcac cgcgccctgc cgccggtggg ccgcccgcgg tacgcgatcc
     4501 cctatttcat gggcccgcat ctggacaccg agatcgcgtg cctgcccacc tgccgggggc
     4561 cggagaaccc accgcgccac ccacccatca cgtacgcggc gtacctcgac tggtggtacg
     4621 acgccaacta caatgcgtct gaccagcgcg acgcggctgc ggcacgccag cgcgacgtgg
     4681 ttgcctcaga ccagcgcgac gtggctgcgg cacgccagcg cgacgtggcc tgaacccgcg
     4741 catcggccag gaggcggaca tgaaggtcgg aatcgcgctg aacatgctga cccaggaagg
     4801 ccggccggac gcggccgtgc tcggcgacca tctggcgctc ggtgacctcg ccgagccgct
     4861 cgggttcgat tcgctcttcg cgctggagca ccacttcacc ggctacgcca tgtcgccctc
     4921 tccgacccag ctcctgagct acttcgcggg ccggacgaag cgcgtcaccc tcggcaccgc
     4981 ggtgatcgtg ctgccgtggc acgatccggt ccggatcgcc gaggagatcg cgctgctcga
     5041 catcctgtcg ggcggtcgat gcctcctcgg gttcggtcgg ggcgccgcga gcgtcgagta
     5101 cgccggattc cggatcccga tggaggaggc acgcgcccgc ttcgtcgaga ccgcgcagat
     5161 cgtggtccgg gccctcagcc aggacgtctt cgagtgggac ggggaattct tcaagatccc
     5221 gcggacgtcc attcgcccgc gcccgatctc ccacccggag cgccggatct acgcctcctc
     5281 ggtgagcccc gaatcggccg agatcatggc gaagctcgga ttcggggtgc tggtcatcat
     5341 gcagaacgag tggccgaagg cggccgagga catccagcgc taccgggaga tcgcgcagag
     5401 cgtcggccac gcgccgcggc cgccgatcat cctcaccaac gtgtcggtgg ccgagagccg
     5461 gacggaggcc cacgagcgag cggtgaagta tctcagccgc aagttcgact cgatcgatac
     5521 ccactaccgc ttctcggacg ggcatctcgg ctcggtcaag ggctacgagt cgtacggcgc
     5581 gaccgccaag acctacgcca agatgaagga tgccaacttc cggcagaagg cggcggactt
     5641 ctacgtgaag atccaggtcg tcggtacccc cgacgagtgc ctgcagcagc tgtcggagct
     5701 ccgccggctg accggcctcg accacctggt caccgagttc gcc
//
LOCUS       DATMCV010000925         6223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_116540, whole genome shotgun sequence.
ACCESSION   DATMCV010000925 DATMCV010000000
VERSION     DATMCV010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116540"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VNU03_21105"
     CDS             complement(<1..425)
                     /locus_tag="VNU03_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77033.1"
                     /translation="MSGSDASSGPLARLGADPRDADAVLQTVRFELYRENIYGALEMV
                     EAARAVHPDPRYSEQAARIRSWLGHLASREAYIAAQDEQYRRLRWRMGLKLIEKRIRM
                     LLGRKTRKMIERRGRDPEFQALEREVAEVRPRRVLDAGSG"
     gene            complement(425..3523)
                     /locus_tag="VNU03_21110"
     CDS             complement(425..3523)
                     /locus_tag="VNU03_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP77034.1"
                     /translation="MSTDLFIRRPVMTTLLMAGILVFGIMAYRLLPVSDLPNIDFPTI
                     QVTASLPGASPDTMASAVATPLEKQFSTIAGIDSMTSTSALGNTQITIQFTLERNIDA
                     AAQDVQAAIAAAQPQMPPGMPTPPSYKKVNPADQPILYLALSSPTLPLYVVDEYAQTN
                     LSQRISTIPGVAQVGIFGSQKYAVRAQLDPSAMATLGIGIDEVQKALADSNVNLPTGT
                     LWGPNQAFSVQATGQLTSAAAYRPLIVAYRNGSPVRLEQLGRVLDGVQTDKVASWYND
                     ERAVVLAIQKQPGTNTIEVVDSINRILPAFRAQLPAAVNLNTLYDRSVSIRSSVHDVQ
                     FTLLLAIALVVLVIFLFLRNVSATLIPSLALPMSIVGTFMVMYVLGYTIDNLSLMALT
                     LAVGFIVDDAIVMLENIVRHMEEGMGRMEAALVGAREIGFTIVSMTASLAAVFLPVLF
                     MGGIVGRLLHEFAVVIGAAVIVSGVVSLTLTPMACSRFLRPPTESHGRVYAASERVFA
                     GMLGLYERTLKLVLRHPRFTFMVFVATVALTAVLFVIVPKGFIPNEDTGQVFAFTEAS
                     QDISFEGMMEHQLKAAAIVRQQPYVKQFFSAIGASGSSVVMNTGRIFMLLKPASERGT
                     ADEIVQDLRKKLSGIPGMNVYPQVLPTIRIGGNLTKGQYQYTMQDADLQTLYTSAPIM
                     YDRMKGLAGFLDVNSDLQITSPQVLVEIDREKASALGVTAVQIENALNNAYGAPQVST
                     IYTPTNQYWVMMELLPQYQRDPAALSMLYIRSSSGKLVPLGTVAKLTRTVGPLTVNHL
                     GQLPAVTISFNLRPGVALGEAVEKIQGVEREVRLPATLSTSFQGTVQAFQRSLQGLGV
                     LLLAAVLVIYLILGILYESFIHPLTILSGLPSAGVGALLTLLIFHEELNLYGFVGIIM
                     LVGIVKKNAIMMIDFAIDAQRKGKPAAEAIYEGCLLRFRPIMMTTMAALMGTLPIAVG
                     VGAGADSRRSLGLAVVGGLVLSQLLTLYITPVIYLYLERFRERATRRREAVPDMAPRP
                     IA"
     gene            complement(3520..4788)
                     /locus_tag="VNU03_21115"
     CDS             complement(3520..4788)
                     /locus_tag="VNU03_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWP77035.1"
                     /translation="MARGRRVVAGAALAGALLTGALLAGCDRRGDTAAAKGVSPGVPV
                     TVADVVTRDVPVQIRAIGNVQPLATVSVLSMINGEVMKVHFGEGQEVPAGSLLFTIDP
                     RQIQAALLQAQATLAQHQAMVKQAEANLAKDTAQLENAKVEEQRYKKLVDGGFVAREQ
                     YDQIKTTEQTLSATIEADRAAIETAKALVRADEAAVENVRVQLSYTEIRAPLDGRTGN
                     LLLHQGNVVKANDVGNPMVVINRIHPIYVTFSVPEAQLAEIKRYRALGDLPVDVQAPG
                     APGGVVRGRLTFVNNTVDPSTGTIQLKATFENTENALWPGQFVNAALTLTRQPGAVVV
                     PSQAVQSGQKGQYVFVVKADNTVEARTVVPGAADGRDVVITSGLQAGERVVTDGQLRL
                     VPGARVDAKTAAPEPAPGAAPKAPAGKAGG"
     gene            complement(4788..5438)
                     /locus_tag="VNU03_21120"
     CDS             complement(4788..5438)
                     /locus_tag="VNU03_21120"
                     /inference="COORDINATES: protein motif:HMM:NF037675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWP77036.1"
                     /translation="MPSATRVKRSLTGHVRDPQRTQDRILAAALAEFSAEGFAGARVA
                     RIARRARVNKRMLYHYFGNKEDLFRGIFDRKLVQRAAWLTEAPPDLGASLAYWFQLAC
                     EDSDWIRLVQWEALGLADQPVIREDERRASFARALGDLRARQRRGLLPADLDAGHLLL
                     AIMGLVAYPLAFPQLTRMVTGRRATDPGFRAERAEFLRRFAAALTGQRPEAAGASA"
     gene            complement(5522..6154)
                     /locus_tag="VNU03_21125"
     CDS             complement(5522..6154)
                     /locus_tag="VNU03_21125"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWP77037.1"
                     /translation="MKAFACVLGVMLAATTWSAPAAALQITFDDLASLGNPLVTTVET
                     HGYRLAGPFLRTIDMPGTTFVGNGSAVYLAQNGAAPGGGVTLARIDGTPFDLYGFSAS
                     GLFAAPGPGSPNASLLAVVAMQVGGGGLSAIYDLGTFAGFVSFPVPATWTNLEFVTFA
                     GIFSATMAGGLALDDIGVGLGPSVPEPATIILALTTIAGLAATTLRRRRS"
BASE COUNT          936 a         2100 c         2269 g          918 t
ORIGIN      
        1 ccgctgcccg cgtccagcac gcggcgcggc cgcacctcgg ccacctcccg ctccagtgcc
       61 tggaactcgg gatcccgccc gcgacgctcg atcatcttgc gggtcttgcg gccgagcagc
      121 atgcggatgc gcttctcgat cagcttgagg cccatgcgcc accgcagccg acggtactgc
      181 tcgtcctgcg ccgcgatgta ggcctcgcgg ctcgccaggt ggccgagcca ggagcggatg
      241 cgggcggcct gctcgctgta ccgcggatcc gggtggacgg cgcgggcggc ctccaccatc
      301 tcgagcgcgc cgtagatgtt ctcgcggtag agctcgaagc gaaccgtctg cagcaccgcg
      361 tcggcgtccc ggggatcggc tccgagccgc gccagcgggc cgctcgacgc gtcgctgccc
      421 gacatcaggc gatggggcgc ggggccatgt ccggcacggc ctcgcgccgg cgggtcgccc
      481 gctcccggaa gcgctccagg tagaggtaga tgaccggcgt gatgtagagg gtcaggagct
      541 gcgagagcac caggccgccg accaccgcga ggccgagcga ccgccgcgag tcggcgcccg
      601 cccccacgcc caccgcgatc ggcagcgtgc ccatgagggc ggccatcgtg gtcatcatga
      661 tggggcggaa gcggagcagg cagccctcgt agatcgcctc ggcggcgggc ttgcccttgc
      721 gctgggcgtc gatggcgaag tcgatcatca tgatcgcgtt cttcttcacg atgccgacca
      781 gcatgatgat cccgacgaag ccgtagaggt tcagctcctc gtggaagatc agcagggtca
      841 ggagcgcgcc cacgcccgcc gacggcagcc cggagaggat ggtcagcggg tggatgaagc
      901 tctcgtagag gatgcccagg atcaggtaga tcacgagcac cgcggccagc agcaggacgc
      961 cgaggccctg cagggagcgc tggaaggcct gcaccgtgcc ctggaagctc gtgctgagcg
     1021 tggccggcag ccgcacctcg cgctccacgc cctggatctt ctccaccgcc tcgccgaggg
     1081 ccacgcccgg gcgcaggttg aaggagatgg tcaccgcggg gagttggccc agatggttga
     1141 cggtcagcgg gccgacggtg cgggtcagct tcgcgaccgt gccgagcggc accagcttgc
     1201 cgctcgagga ccggatgtag agcatggaca gcgcggccgg gtcgcgctgg tactgcggta
     1261 ggagctccat catcacccag tactggttgg tgggagtgta gatggtggag acctgcggcg
     1321 ccccgtaggc attgttgagc gcgttctcga tctgcaccgc ggtcaccccg agagcggagg
     1381 ccttctcgcg gtcaatctcc accagcacct gcgggctggt gatctggaga tcggagttca
     1441 cgtcgaggaa gccggccagc cccttcatgc ggtcgtacat gatcggggcc gaggtgtaga
     1501 gggtctgcag atcggcgtcc tgcatcgtgt actggtactg gcccttggtc aggttgccgc
     1561 cgatgcggat ggtgggcagc acctgcgggt agacgttcat gccggggatg ccggagagct
     1621 tcttgcgaag gtcctgcacg atctcgtcgg cggtgccccg ctcggaggcg ggcttcagca
     1681 gcatgaagat gcgcccggtg ttcatcacca cgctcgagcc gctggccccg atggcggaga
     1741 agaactgctt cacgtagggc tgctgccgca cgatcgcagc cgccttcagc tggtgctcca
     1801 tcatgccctc gaaggagatg tcctgcgagg cctcggtgaa ggcgaagacc tggccggtgt
     1861 cctcgttggg gatgaagccc ttgggcacga tgacgaagag gacggcggtc agggccaccg
     1921 tcgcgacgaa gaccatgaag gtgaagcggg ggtggcgcag cacgagcttc aaggttcgct
     1981 cgtagaggcc gagcatgccc gcgaagacgc gctccgacgc ggcgtagacg cgcccgtgcg
     2041 actcggtggg cgggcgcagg aagcggctgc acgccatcgg ggtgagggtc agcgagacca
     2101 cgcccgagac gatgaccgcg gcgccgatca ccaccgcgaa ctcgtggagg agacgcccca
     2161 cgatgccgcc catgaacagc accggcagga acaccgccgc gagcgaggcg gtcatcgaga
     2221 cgatggtgaa gccgatctcc cgcgccccga ccagggccgc ttccatccgg cccatcccct
     2281 cctccatgtg gcgcacgatg ttctcgagca tcacgatcgc gtcgtccacg atgaagccca
     2341 ccgcgagggt cagcgccatc agggagaggt tgtcgatcgt gtagccgagc acgtacatca
     2401 ccatgaacgt gcccacgatc gacatgggca gggccaggct cgggatgagg gtcgccgaca
     2461 cgttgcgcag gaagaggaag atgaccagca ccaccagcgc gatcgcgagc agcagcgtga
     2521 actgcacatc gtggaccgag gaccggatcg agaccgagcg gtcgtagagg gtgttcaggt
     2581 tcaccgccgc gggcagctgg gcccggaagg cgggcaggat ccggttgatc gagtccacca
     2641 cctcgatggt gttggtgccc ggctgcttct ggatcgccag caccaccgcc cgctcgtcgt
     2701 tgtaccagga ggcgaccttg tcggtctgca ccccgtccag cacccggccg agctgctcga
     2761 gccgcaccgg ggagccgttg cgatacgcga cgatcaatgg ccggtaggcg gccgcgctcg
     2821 tgagctggcc ggtggcctgc acgctgaagg cctggttcgg gccccagagg gtgccggtgg
     2881 gcaggttcac gttggagtcg gcgagggcct tctgcacctc gtcgatgccg atgcccaggg
     2941 tggccatcgc gctcggatcg agctgggccc gcaccgcgta cttctgcgag ccgaagattc
     3001 ccacctgggc cacgcccgga atggtggaga tgcgctgcga gaggttggtc tgcgcgtact
     3061 cgtccaccac gtagagcggc agcgtcggcg agctgagcgc caggtagagg atcggctgat
     3121 cggccggatt gaccttcttg taggacggcg gcgtcggcat gccgggcggc atctggggct
     3181 gggccgccgc gatggcggcc tgcacgtcct gggccgccgc gtcgatgttg cgctcgaggg
     3241 tgaactggat cgtgatctgg gtgttgccga gcgcgctcgt cgaggtcatc gagtcgatgc
     3301 ccgcgatggt ggagaactgc ttctcgagcg gcgtcgcgac cgcggaggcc atggtgtcgg
     3361 ggctcgcgcc cggcagcgac gcggtcacct ggatggtcgg gaagtcgatg ttgggcaggt
     3421 cgctcaccgg caggagccgg taggccatga tcccgaagac caggatgccg gccatgagca
     3481 gcgtggtcat gaccggccgg cggatgaaga ggtcggtgct catccacccg ccttcccggc
     3541 cggcgccttg ggcgcggcgc ccggcgcggg ctcgggcgcc gcggtcttgg cgtccacccg
     3601 cgcgcccggt accagccgga gctgcccgtc cgtcaccacc cgctcgcccg cctggagccc
     3661 gctcgtgatc acgacgtcgc gcccgtcggc ggcgcccggc accaccgtac gcgcctccac
     3721 cgtgttgtcc gccttcacca cgaagacgta ctggcccttc tgcccgctct gcaccgcctg
     3781 cgacgggacc accaccgcgc cgggctggcg ggtgagggtc agcgccgcgt tgacgaactg
     3841 ccccggccag agcgcgttct cggtgttctc gaaggtggcc ttgagctgga tggtgccggt
     3901 gctgggatcc accgtgttgt tcacgaaggt gagccgcccc cgcaccacgc cgcccggcgc
     3961 gcccggcgcc tgcacgtcga ccggcagatc gcccagcgcg cggtaacgct tgatctcggc
     4021 cagctgcgcc tcgggcaccg agaaggtcac gtagatgggg tggatgcggt tgatcaccac
     4081 catcgggttg cccacgtcgt tggccttcac cacgttgccc tggtgcagca gcaggttgcc
     4141 ggtgcggccg tcgagggggg cgcggatctc ggtgtaggag agctgcaccc gcacgttctc
     4201 caccgcggcc tcgtcggccc gcacgagggc cttggcggtc tcgatcgcgg cgcggtcggc
     4261 ctcgatggtg gcgctcagcg tctgctcggt ggtcttgatc tggtcgtact gctcgcgggc
     4321 cacgaagccc ccgtcgacca gcttcttgta gcgctgctcc tcgaccttgg cgttctcgag
     4381 ctgggcggtg tccttggcca ggttggcctc ggcctgcttc accatcgcct ggtgctgggc
     4441 gagcgtggcc tgggcctgca gcagcgccgc ctggatctgg cgcgggtcga tggtgaagag
     4501 cagggacccg gcgggcacct cctgcccctc gccgaagtgc accttcatca cctcgccgtt
     4561 gatcatcgag agcaccgaca cggtggcgag cggctgcacg ttgccgatgg cgcggatctg
     4621 caccggcacg tcgcgcgtga ccacgtcggc caccgtcacc ggcacgccgg gagacacgcc
     4681 cttggcggcc gcggtgtctc cgcgccggtc gcacccggcc agcagcgcgc cggtgaggag
     4741 ggcgccggcg agcgccgccc ccgccacgac ccgacggccc cgcgccatca cgcgctcgct
     4801 cccgcggcct cgggccgctg cccggtcagg gccgccgcga agcgccggag aaactccgcg
     4861 cgctcggccc ggaagcccgg atcggtcgcg cgccgcccgg tgaccatgcg ggtgagctgg
     4921 gggaaggcca gcgggtatgc gacgagcccc atgatcgcca gcagcagatg gcccgcgtcg
     4981 aggtcggccg gcaggaggcc gcgccgctgg cgcgcgcgca ggtcccccag ggcccgggcg
     5041 aacgacgcgc ggcgctcgtc ctcgcgaatc accggctggt cggccaggcc gagcgcctcc
     5101 cactggacga gccggatcca gtcgctgtcc tcgcaggcca gctggaacca gtaggcgagg
     5161 ctcgccccca ggtcgggcgg ggcctcggtg agccacgccg cccgctggac gagcttgcgg
     5221 tcgaagatcc cgcggaagag atcttccttg ttgccgaagt agtgataaag catgcgcttg
     5281 ttcacgcgcg cccgccgggc gatgcgcgcg acccgggcgc cggcgaagcc ctcggccgag
     5341 aactcggcga gggcagcggc gagaatccgg tcctgtgtcc gctgagggtc gcggacgtgg
     5401 cccgtcaggg agcgcttcac acgggtggcc gacggcatcg ggtgatcgac ccgatagtaa
     5461 ccgtttggat acttctcggt caagccttgg gctggtgggc cggcccaggg ggagcggggg
     5521 actaggagcg gcgcctgcgg agggtggtgg cggcgagccc cgcgatggtg gtgagcgcca
     5581 ggatgatcgt ggccggctcc ggcacgctgg ggcccaggcc cacgccgatg tcgtccaggg
     5641 cgagcccccc ggccatggtg gccgagaaga tgccggcgaa cgtcacgaac tcgaggttgg
     5701 tccaggtcgc cggcaccggg aagctcacga agccggcgaa ggtcccgagg tcgtagatgg
     5761 cgctcagccc gcccccgccg acctgcatcg cgaccacggc cagcagggaa gcgttcggcg
     5821 agccgggccc gggggcggcg aagaggccgg acgcggagaa gccgtacagg tcgaaggggg
     5881 tgccgtcgat ccgcgccagg gtgacgccgc cgccgggcgc ggccccgttc tgggcgaggt
     5941 agaccgccga gccattcccg acgaacgtcg tccccggcat atcgatcgtg cgcaggaacg
     6001 gccccgcgag gcggtagccg tgggtctcga ccgtcgtgac gagcgggttg ccgaggcttg
     6061 ccaggtcgtc gaaggtgatc tggagcgcag cggcgggcgc cgaccacgtc gtcgccgcga
     6121 gcatcacacc gagcacgcac gcaaaggctt tcatcgttgg ccccctccaa gagcgacccc
     6181 ctcgcaggca aggcgcttgc catgcgacgc acgacgcaag atg
//
LOCUS       DATMCV010000926         6397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_847900, whole genome shotgun sequence.
ACCESSION   DATMCV010000926 DATMCV010000000
VERSION     DATMCV010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847900"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VNU03_21130"
     CDS             <1..682
                     /locus_tag="VNU03_21130"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013961646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77038.1"
                     /translation="FPPPALSLRWYRSLFDPVQSAQIHTAVWNSLVIAFSATAIAVTL
                     GTAAALGIAGRRAWWAQALDGLFMSPLVLPTLAFGLAALMFFSTLGFPLSIPLMILGH
                     VAVIVPFVLRTTIASLSQLDRGLLDTSASLGAGAFYTFRRVTLPIIRPGILAGAFIAF
                     MASLDNVSVSLFLAGPENDVLPIRMWSMLESTLDVRTAAVSGVLIFSTALLVVVMDRI
                     VGLTRRIS"
     gene            682..1167
                     /locus_tag="VNU03_21135"
     CDS             682..1167
                     /locus_tag="VNU03_21135"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HWP77039.1"
                     /translation="MRIVAEPLTAEAFKPFGAVLEGTPAPGRAYLSDTLANRRAHAPV
                     SLAVATVEPKATFPLDVKVLERHEHSSQTFIPLHVSRYLVLATLDAPGGGPDLSRLRA
                     FVARAGQGVTYSMGTWHHPLTVLDGPASFAVLMWRDGTPGDEEFVPVTTPLTIDLPQE
                     P"
     gene            1171..2082
                     /locus_tag="VNU03_21140"
     CDS             1171..2082
                     /locus_tag="VNU03_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HWP77040.1"
                     /translation="MPTDFARDLVGYGGTPPHPHWPGEARIAVNFVMNYEEGSEYNAL
                     DDGVSEATLTESGASNYGVKGRDLAAEGMFEYGSRVGFWRVHRLFKERGLPLTVFGCA
                     LALERNPKVAAAIRDSGWDVCSHGWRWVKHYELSEAEEREHIRKAIESMKKTVGERPL
                     GWYCRYGPSVNTRRLIVEEGGFLYDSDAYNDELPYWVRVGSKPHLVVPYSLTANDSKF
                     GSGGFFTAADYFTFVKDGFDVLYREGQTQPKMMSLGLHLRLIGHPSRAAGLERALDYI
                     AGHKDVWFTRRIDIARHWATTHPAPAR"
     gene            complement(2185..3030)
                     /locus_tag="VNU03_21145"
     CDS             complement(2185..3030)
                     /locus_tag="VNU03_21145"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWP77041.1"
                     /translation="MPKVLTEAQVERYQRDGCLSPVRAMSAERARYYREKFEALESRV
                     PDIKKMKTKSHLLAPWVLEIAEDPHILDVFEDLVGSNLRCWSMAWRVKKADGETFAGW
                     HQDSAYGAAQPVVLGGLALSNCGVTQGCLRGVPGSHKWGILKHDEKDDPKSILAKGQF
                     ITDEFDESKAVDFPLEPGEMVMFDNSLVHGSGTNFGPDRRFLLLVEMVPTWAKPARVR
                     QSAMLVRGTDTSGNFDDEPRPDAEWSDYAVGNWNRVVESRAKLIFENSKVAPSVAYGG
                     TRAAT"
     gene            complement(3117..4232)
                     /gene="alr"
                     /locus_tag="VNU03_21150"
     CDS             complement(3117..4232)
                     /gene="alr"
                     /locus_tag="VNU03_21150"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWP77042.1"
                     /translation="MTAPLRPTTLEIDLDAAADNLRAVRGLVGPSRSIFAVVKADGYG
                     FGAAEMGAVFARSGADWLAVADLSEGVRLRERGITIPILVYPNSLPEAAAAALAHRLV
                     PTLVDLESARAYSQAASGPCPVFVKVDVGLERLGVPAEQAVKTILAMLELPHLTLGGL
                     CAHPHAEVGGDSAYADWQAGRFTAVVDELEARGVRVPVRLLAASPFVLRFPHTYLNAV
                     DPGRMLYGITFPGETPPVPLRPALRALATRVIALKELAPRERFAEAAPFPVTGPMRLG
                     VVPMGSADGLRWLHAGRVLVRGRAVPLIGTPSLEHTRIDLTAVPDAAVGDEVIIIGRQ
                     GDLEITPAEVAAHHGLGLHHVATTVGPRVARIYHGAA"
     gene            complement(4234..5001)
                     /locus_tag="VNU03_21155"
     CDS             complement(4234..5001)
                     /locus_tag="VNU03_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWP77043.1"
                     /translation="MRANKIKQMWREGKCVTMGWLSVSNGFTAEVMARQGFDALVIDM
                     QHGLTDMANLWPMLQAVSQTDTVPVVRVAANDPATIMKALDFGAYAILVPLINTAEDA
                     AKAVAACRYPPVGMRSSGPVRAVHYGGADYVAKANDEIVVMAMIETKEGLANLDAICA
                     TPGLDCIYIGPADLSFALGLAPRGDNPDPVHIATCDKIRDAAHKHGKKAAMHCASAAF
                     AAGSVKRGFDLVMLCSDLASMVAGVRKQLDDLKAATA"
     gene            5101..5892
                     /locus_tag="VNU03_21160"
     CDS             5101..5892
                     /locus_tag="VNU03_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77044.1"
                     /translation="MDLELRDKVAIVGGASKGLGRACAEVLAQEGAQVAICSRSKDDL
                     EKAASEIRAATGREVLVFAGDLDRPETIRDLVAATVARFGRLDVLVNNSGGPPLAQAQ
                     SATEEQWETAVQRSLFFFGRMCREALPHLKRQRAGRIINILASTVYQPIPNLVLSGVT
                     RMGVVAFAKTLADEVGRDGILVNNVCPGSIMSERMLSNVTARAKQLGIPLEEALAQRA
                     AETAVGRVGEPKELAHLVAFLASGKSSYITGTTILVDGGLVRSIL"
     gene            5902..>6397
                     /locus_tag="VNU03_21165"
     CDS             5902..>6397
                     /locus_tag="VNU03_21165"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWP77045.1"
                     /translation="MTPLPQVDAVLRRAAESREVSGVVAMAATDRGVLYEGAFGRRAL
                     GQPEPMTLDSVFRIASMTKAITSVAAMQLVEQGKLALEAPVPDIDPTLGSPQVLEGFD
                     ASGTPRLRPARRPITLRHLLTHTAGFGYSVWDPVVGRHVETAGLPARGGGHVASIRMP
                     LVFDP"
BASE COUNT          908 a         2329 c         2203 g          957 t
ORIGIN      
        1 cttccccccg cccgccctgt cgctccgctg gtaccggagc ctcttcgacc cggtccagtc
       61 cgcccagatt cacaccgcgg tgtggaacag cctggtgatc gcgttctccg ccaccgccat
      121 cgcggtgacg ctcggcaccg cggccgccct cggcatcgcg gggcggcgcg cgtggtgggc
      181 gcaggccctc gacgggctct tcatgtcccc gctcgtcctg cccaccctgg ccttcgggct
      241 cgccgcgctc atgttcttca gcaccctcgg cttcccgctc tcgatcccgc tgatgatcct
      301 gggccacgtg gcggtcatcg tgccgttcgt cctgcgcacc accatcgcca gcctgtcgca
      361 gctcgaccgc gggctgctcg acacctcggc gagcctgggg gcgggcgcct tctacacctt
      421 ccggcgggtc accctgccca tcatccggcc gggcatcctg gccggggcct tcatcgcctt
      481 catggcctcg ctcgacaacg tgtcggtctc gctcttcctg gccgggcccg agaacgacgt
      541 cctgccgatc cgcatgtgga gcatgctcga gagcacgctc gacgtgcgca ccgccgcggt
      601 gtccggcgtg ctgatcttct ccaccgccct gctcgtcgtg gtcatggacc gcatcgtcgg
      661 cctgacccgg aggatctcct gatgcgcatc gtcgccgagc cattgaccgc ggaagccttc
      721 aagcccttcg gggcggtgct ggagggcacg cccgcgccgg ggcgcgccta tctgagcgac
      781 acgctggcca accggcgcgc ccacgccccg gtgagcctcg cggtcgccac cgtggagccg
      841 aaggccacct tccccctgga cgtgaaggtc ctcgagcgcc acgagcactc gtcgcagacc
      901 ttcatcccgc tccacgtgtc gcgctacctg gtgctggcca ctctcgacgc gcccggcggc
      961 gggcccgatc tctcgcgcct gcgcgccttc gtggcgcgcg cgggccaggg cgtcacctac
     1021 tccatgggca cgtggcatca cccgctcacg gtgctggacg ggcccgcctc gttcgcggtg
     1081 ctcatgtggc gcgacggcac cccgggtgac gaggagttcg tgccggtcac cacaccgctc
     1141 accatcgatc ttccccagga gccctgagac atgcccaccg atttcgcgcg cgacctcgtc
     1201 ggctacggtg gcactccccc gcatccccac tggccggggg aggcgcggat cgcggtcaac
     1261 ttcgtcatga attacgagga gggctccgag tacaacgcgc tcgacgacgg cgtctcggag
     1321 gccacgctca ccgaatccgg cgcctccaac tacggggtca aggggcgcga cctcgccgcc
     1381 gaggggatgt tcgagtacgg cagccgggtg ggcttctggc gcgtgcaccg gctcttcaag
     1441 gagcgcggcc tgccgctcac cgtgttcggg tgcgcgctcg cgctcgagcg caacccgaag
     1501 gtcgcggccg cgatccgcga ctcgggctgg gacgtgtgct cccacggctg gcggtgggtc
     1561 aagcactacg agctgtccga ggcggaggag cgggagcaca tccgcaaggc gatcgagtcg
     1621 atgaagaaga cggtgggcga gcggccgctc ggctggtact gccgctacgg cccgagcgtg
     1681 aacacccggc ggttgatcgt ggaggagggc ggcttcctct acgactccga cgcctacaac
     1741 gacgagctgc cctactgggt gcgggtgggg agcaagcccc acctggtcgt gccgtacagc
     1801 ctcaccgcca acgactccaa gttcggcagc ggtggcttct tcaccgccgc cgactacttc
     1861 acgttcgtga aggacggctt cgacgtgctc taccgtgagg gccagaccca gcccaagatg
     1921 atgtcgctcg gcctgcacct gcggctgatc ggccacccgt cacgggccgc cggcctcgag
     1981 cgcgcgctgg actacatcgc gggccacaag gacgtctggt tcacgcgccg catcgacatc
     2041 gcccgccact gggccaccac ccaccccgcc cccgcccgct gatggggccc gctgatgggg
     2101 tcaggtctta cattccgaca ttttcatcgc ctggggcgat atgacgcgct ccacgaaaaa
     2161 tgtcggtgta agacctgacc ccgcctaagt cgcggcgcgg gtgccaccgt aggcgacgct
     2221 gggggcgacc ttgctgttct cgaagatgag cttggcgcgc gactcgacca cccggttcca
     2281 gtttccgacc gcgtagtccg accactccgc gtcgggccgc ggctcgtcgt cgaagttgcc
     2341 ggaggtgtcg gtcccgcgca ccagcatcgc ggactgacgg acgcgcgcgg gcttggccca
     2401 cgtcggcacc atctcgacca gcagcaggaa gcgccggtcg gggccgaagt tcgtgccgga
     2461 gccgtggacc agcgagttgt cgaacatcac catctcgccg ggctcgagcg ggaagtcgac
     2521 cgccttcgac tcgtcgaact cgtcggtgat gaactggccc ttggcgagga tggacttggg
     2581 gtcgtccttc tcgtcgtgct tcaggatccc ccacttgtgc gagccgggca ccccgcgcag
     2641 gcatccctgg gtcacgccgc agttggagag cgcgagcccg cccagcacca ccggctgcgc
     2701 ggccccgtac gcggagtcct ggtgccagcc cgcgaaggtc tcgccgtcgg ctttcttcac
     2761 ccgccaggcc atggaccagc agcggaggtt cgagccgacc aggtcctcga agacgtcgag
     2821 gatgtgcggg tcctccgcga tctcgagcac ccagggggcg agcaggtgcg acttggtctt
     2881 catcttcttg atgtcgggga cgcgcgactc gagcgcctcg aacttctcgc ggtagtagcg
     2941 cgcccgctcc gcgctcatcg cgcgcaccgg ggagagacag ccgtcgcgct gataccgctc
     3001 cacctgggct tcggtcagga ccttgggcat gtggtgagcc tcctcatcgc cggccggaaa
     3061 aatgtcggcc aaaaaatgtt ggaatgtaag acctgacccc atcaggaggc gccccatcag
     3121 gcggcgccgt ggtagatgcg ggcgactcgg gggccgacgg tcgtggccac gtggtgaagg
     3181 ccgaggccgt ggtgggcggc cacctcggcg ggggtgatct cgagatcgcc ctggcggccg
     3241 atgatgatga cctcgtcgcc gaccgccgcg tccggcaccg cggtgaggtc gatgcgggtg
     3301 tgctcgagcg acggcgtccc gatcaggggc accgcacggc cccgtaccag gacgcggccg
     3361 gcgtggagcc agcgcagccc gtcggccgag cccatcggca ccaccccgag ccgcatcggc
     3421 ccggtcaccg ggaacggcgc cgcctcggcg aaccgctcgc gaggggccag ctccttcagc
     3481 gcgatcaccc gcgtggcgag cgcgcgcagc gccggccgca ggggcaccgg cggcgtctcc
     3541 ccggggaacg tgatgccgta gagcatccgg cccggatcga ccgcgttcag ataggtgtgc
     3601 gggaagcgca gcacgaacgg gctcgcggcc aggagccgca ccggcacccg caccccgcgc
     3661 gcctccagct cgtcgaccac cgcggtaaag cggcccgcct gccagtcggc gtaggcggaa
     3721 tcgccgccca cctcggcgtg cgggtgcgcg cagaggccgc ccagggtgag gtgcggcagc
     3781 tccagcatcg cgaggatggt cttgaccgcc tgctcggccg ggaccccgag ccgctcgagc
     3841 ccgacgtcca ccttgacgaa caccgggcac gggccggacg ccgcctgcga gtaggcgcgg
     3901 gcggactcga gatcgacgag ggtcggcacg aggcgatggg cgagcgccgc ggcggccgcc
     3961 tccgggagcg agttcggata gacgaggatg gggatggtga tgccgcgctc gcggagccgc
     4021 accccctcgg agagatcggc caccgcgagc cagtccgcgc cgctgcgggc gaagaccgcg
     4081 cccatctccg cggcgccgaa gccgtacccg tcggccttga ccaccgcgaa gatggaccgc
     4141 gacgggccga cgagcccgcg aaccgcgcgc agattgtccg ccgccgcatc gagatcgatc
     4201 tcgagcgtgg tcgggcgaag gggcgcggtc atctcaagcg gtagcggcct tcaggtcgtc
     4261 gagctgcttg cgcacgcccg cgaccatgga cgcgagatcg gagcagagca tgaccaggtc
     4321 gaagccgcgc ttcaccgagc ccgccgcgaa ggccgcgctc gcgcagtgca tggccgcctt
     4381 cttgccgtgc ttgtgggccg cgtcgcggat cttgtcgcag gtggcgatgt gcaccgggtc
     4441 cgggttgtcg ccgcgcgggg ccagcccgag cgcgaacgag aggtcggcgg ggccgatgta
     4501 gatgcagtcg aggcccgggg tggcgcagat cgcgtccagg ttggcgaggc cctccttggt
     4561 ctcgatcatc gccatcacca cgatctcgtc gttggccttg gccacgtagt cggcgccgcc
     4621 gtagtgcacc gcgcgcaccg gacccgagga gcgcatgccc accgggggat agcggcaggc
     4681 ggccaccgcc ttcgcggcgt cctccgcggt gttgatcagc ggcaccagga tggcgtacgc
     4741 cccgaagtcg agcgccttca tgatggtggc ggggtcgttc gcggccaccc gcaccaccgg
     4801 caccgtgtcg gtctgcgaga ccgcctgcag catgggccag aggttggcca tgtcggtgag
     4861 gccgtgctgc atgtcgatga ccagggcgtc gaagccctgc cgggccatga cctcggcggt
     4921 gaacccgttc gacaccgaga gccagcccat cgtcacgcac ttgccctcgc gccacatctg
     4981 cttgatcttg ttcgctcgca tcgcgtcaca cctcgtcgtg taggggatcc cgtgggggta
     5041 gccggcttcg cccggcgcgc ggggaagcgt atcacagccc ctgtggtata acactgcccc
     5101 atggacctcg agctccggga caaggtggcg atcgtcggcg gcgccagcaa gggcctcggc
     5161 cgggcctgcg cggaggtgct cgcgcaggag ggcgcgcagg tggccatctg cagccgctcg
     5221 aaggacgatc tggagaaggc cgcgagcgag atccgcgccg cgaccggccg cgaggtgctg
     5281 gtcttcgcgg gcgacctgga ccggcccgag accatccggg acctggtggc ggccaccgtg
     5341 gcccgcttcg ggcgcctcga cgtcctggtg aacaactcgg gcggcccgcc cctggcccag
     5401 gcccagagcg cgacggagga gcagtgggag accgcggtgc agcgctcgct cttcttcttc
     5461 gggcggatgt gccgcgaggc cctgccgcat ctcaagcgcc agcgcgccgg ccgcatcatc
     5521 aacatcctgg ccagcaccgt ctaccagccc atccccaacc tggtgctctc cggcgtcacc
     5581 cgcatgggcg tggtcgcgtt cgccaagacg ctggccgacg aggtcggccg cgacggaatc
     5641 ctggtcaaca acgtgtgccc cggctcgatc atgagcgagc gcatgctgtc caacgtgacc
     5701 gcgcgcgcca agcagctcgg catcccgctc gaggaggcgc tggcccagcg cgcggccgag
     5761 accgcggtcg gccgcgtggg ggagccgaag gagctggccc acctggtggc gttcctcgcc
     5821 tccggcaagt cgagctacat caccgggacc acgatcctgg tcgacggcgg cctcgtccgc
     5881 tccatcctct agaccgatcg catgaccccg ctgccgcagg tcgacgccgt cctccgccgg
     5941 gccgccgaat cccgcgaggt gtcgggcgtg gtggcgatgg ccgctaccga ccgcggcgtc
     6001 ctctacgagg gcgcgttcgg ccggcgcgcg ctcggccagc ccgagccgat gacgctcgac
     6061 agcgtcttcc gcatcgcctc gatgaccaag gccatcacct cggtcgccgc gatgcagctg
     6121 gtcgagcaag gcaagctcgc gctcgaggcg ccggtgcccg acatcgaccc cacgctcggc
     6181 tcgccccagg tcctggaagg cttcgacgca tcgggcacgc cgcgcctccg ccccgcccgg
     6241 cggccgatca cgctgcgcca cctgctgacc cacaccgccg gctttgggta cagcgtgtgg
     6301 gatccggtcg tcggccgcca tgtcgagacc gcggggctgc ccgcgcgggg cggcgggcac
     6361 gtggcgtcca tccgcatgcc gctcgtcttc gatccgg
//
LOCUS       DATMCV010000927         5693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_847904, whole genome shotgun sequence.
ACCESSION   DATMCV010000927 DATMCV010000000
VERSION     DATMCV010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847904"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VNU03_21170"
     CDS             complement(<1..910)
                     /locus_tag="VNU03_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWP77046.1"
                     /translation="MGPDRAATGGTTRQSTAFLPLSQVLEEEFVALHGPLPGPARGVD
                     DGERLPALYEAIHALPERRAALCLSGGGIRSATFALGVLQGLARAGLLTQFHYLSTVS
                     GGGYIGAWLSAWIHRKGNDAEKVALELASLEPRFKLDPEPEPIAHLREFSNYLSPRAG
                     LMSTDTWTLLGTILRNMILIWLVLVPLLAAVLMLPRLYLSLLRWATNATGSTLPTVLL
                     VLGGALLAWAVAYIAATIPSSTTARRGQGKFLAFCLLPLLLGLGSLTIYWAAVSGRAA
                     GAARAPLPVFVEFVWFGAVVHLLAWAG"
     gene            1019..2074
                     /locus_tag="VNU03_21175"
     CDS             1019..2074
                     /locus_tag="VNU03_21175"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77047.1"
                     /translation="MATGPSHIYVGAARWTSGTKSGVFRRADGAGAWELMSKGLPEAA
                     HVQAITVHPGNPDVVYVGTQNGPYRSIDRGERWERLPFPEDGGEVWSILVHPTRPRTL
                     LAGTSPVGIFRSDDEGATWRRLPKAVQVERVKMPFACRVMRLAVDPAQPEHIYAALEV
                     GGAMRSLDGGETWEDGSADLVKLSDLPHLKSRIVSDSEMEGMLDGHALCVSGAASGTV
                     FLALRMGLFRSTDRAAHWQDMEVGRFSPLTYSRDIRVSPQDPRVLYACLSPAARSEDG
                     SLYRSGDLGETWTRFDHGVKAESTMMAVALHPRDAGRVYCASRTGQIFATEDAGRSWR
                     EERLPEGVSDVYAIACG"
     gene            complement(2053..2703)
                     /locus_tag="VNU03_21180"
     CDS             complement(2053..2703)
                     /locus_tag="VNU03_21180"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HWP77048.1"
                     /translation="MNNRTDHGFLVLADVSGFTAFVTTTELEHGSDIIATLLDEVIGH
                     LSPPLEIQEIEGDAVFALGGESFALPHTRLLAVLEEAFAAFKSRQQVMQAAEKCQCGA
                     CQEIGTLDLKMVVHHGPFLRHTVAGRSRVTGTAVILVHRLLKNGVGRKGGYALLTEPV
                     LRSLGIDGERAGLPAHTERYEHLGDVRCFVRDLAPLSLSPEGRGQGEGALYPQAIA"
     gene            complement(2753..3880)
                     /locus_tag="VNU03_21185"
     CDS             complement(2753..3880)
                     /locus_tag="VNU03_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77049.1"
                     /translation="MATITPSTRGLKQVAWLDCPGGGQVSVDGTIAYVAHMKAPHGTS
                     IVDVSDPARPRTLATVEVPPHTHSHKVRAANGLMLVNREAQPAHQPPAGVGGGLVIYD
                     VSTPARPREITFWKCGGAGVHRFTFDGRYAYISPEMDGYVGNIVMILDLHDPTRPKEV
                     GRWWMPGQWTGGGEQPAWSGRHHRCHHPIRAGNRLYVSYWHGGFVILDIEDMAKPRFV
                     SGLDWSPPYITPTHTALPLPFPLHGRRIMLAADEDVAKTAPGPPSFMWLVDITDERHP
                     VPISTFQVAGVDGEPQPEFTGCHQPCERVTGTEIPVAWFAHGLRIVDVANPHEPREIA
                     HFLPEPPAGHSRVSSNDVEVDGRGLLYLIDRGRGLHILERV"
     gene            3993..5561
                     /locus_tag="VNU03_21190"
     CDS             3993..5561
                     /locus_tag="VNU03_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77050.1"
                     /translation="MALSTDRLPEVGNAYARLEDKILERDQGAASDIFYDLVRAGVPI
                     PQLVGQLVRIHAPYTHVPYHQRLDDGIVRFVNNDHCLLSSRAGIDLVPLMPPELRYLP
                     FAQTIWYMPTGLDPWNQLIGKMPGHYVRLYEIKFEGKPPLPEVHWPDQAPLAIEGTWP
                     EKLNTWLTLVQRGEVINAYRVFLGLWDEAAVDPAKRTPLLAQLVFAGLIDVQDRVLYN
                     RSYTTGHKSYRARATVELARAVGWNDAHSILYAGVPDIAVGPRWYSTYEMACQIVQIF
                     LDNRDRELLDNDGALTPVESLALQQALLSGQEPGHIDLIVALLQTGRGLRQIIDTIQL
                     ASAQLIMETGDPNAYSMPQHSYEYCNTVRWFFDSFAHPHRLKLLFVAASFVNRAAHHQ
                     KHTPDNHPVKVTPPKGSDALTPAQLLERLDSALDGLRAFEAMDWTAAYLRSGADRAAL
                     VQTLAVGAVKAGNDPHNQELGICLLHDYGHSTAADRDRLLLASAKHTAGHRKYGSHLD
                     AWRRYADAFGLARS"
BASE COUNT          874 a         2026 c         2013 g          780 t
ORIGIN      
        1 agccggccca ggccagcagg tgcacgacgg ccccgaacca gacgaactcg acgaacaccg
       61 gcaacggcgc gcgggccgcc ccggcggcgc gccccgagac cgcggcccag tagatggtca
      121 aggagcccag cccgaggagc agcgggagca ggcagaacgc gaggaacttg ccctggccgc
      181 ggcgtgcggt cgtgctgctg ggaatggtcg ccgcgatgta cgccaccgcc caggcgagca
      241 gcgcgccccc gagcacgagc aggacggtgg gcagggtgga tccggtcgcg ttggtggccc
      301 agcggaggag cgagaggtag agacgcggca gcatgagaac cgcggcgagc agcggtacca
      361 gcacgagcca gatcaggatc atgttgcgca ggatggtgcc gaggagggtc cacgtgtccg
      421 tcgacatcag cccggcccgt gggctcaggt agttgctgaa ctcgcgcagg tgcgcgatcg
      481 gctccggctc cggatcgagc ttgaagcgcg gctcgagcga ggccagctcg agggcgacct
      541 tctccgcgtc gttgcctttc cggtggatcc acgcgctgag ccaggctccg atgtagccgc
      601 cgccggagac ggtggagagg tagtggaact gggtcaagag cccggcgcgc gccagcccct
      661 ggaggacgcc cagggcgaag gtggcgctgc ggatgccccc gcccgacagg cagagcgcgg
      721 cgcgcctctc gggcagcgca tggatcgcct cgtagagcgc ggggagccgc tccccgtcgt
      781 ccaccccgcg cgccggtccg ggaagcgggc cgtgcagggc gacgaactcc tcctccagga
      841 cctgcgacag ggggagaaag gcggtggact ggcgcgtggt gcctccggtc gcggcgcgat
      901 cagggcccat cccaagcacg ctaggccgtc ccgcgcgcgg gggtcaagga atttgatatg
      961 ctctggcgca cgaagccgct ttaccggaca cgacggtcaa ctggaaaggg gtgcggacat
     1021 ggcgacgggg ccctctcaca tctacgtcgg cgcggcacgg tggacttccg gaacgaagag
     1081 cggcgtcttt cgccgggcgg acggggcggg cgcctgggag ctgatgagca aggggctccc
     1141 ggaggcggcc cacgtgcagg ccatcacggt gcatcccggc aatcccgacg tggtgtacgt
     1201 gggcacccag aacggcccct accggagcat cgaccggggc gagcgctggg agcgcctgcc
     1261 cttccccgag gacggcggcg aggtatggtc catcctcgtg catcccaccc gtccgcggac
     1321 gctcctggcc gggacctcgc cggtcggcat cttccgcagc gacgacgagg gcgccacgtg
     1381 gcgccggctg cccaaggcgg tgcaggtcga gcgcgtgaag atgcccttcg cgtgccgggt
     1441 gatgcgcctg gccgtcgacc ccgcgcagcc cgagcacatc tacgcggcgc tcgaggtggg
     1501 gggcgcgatg cgcagcctcg acggcggcga gacgtgggag gacgggagcg ccgacctcgt
     1561 caagctctcg gacctgccac acctcaagag ccgcatcgtg agcgacagcg agatggaagg
     1621 catgctggac ggccacgccc tctgcgtcag cggggcggcc agcggcaccg tcttcctcgc
     1681 gctccgcatg ggcctcttcc gcagcaccga tcgcgcggcg cactggcagg acatggaggt
     1741 cggccgcttc tcccccctca cctacagccg cgacatccgc gtctcgccgc aggacccgcg
     1801 cgtgctctac gcgtgcctga gcccggctgc gcggagcgag gacggctccc tctaccggag
     1861 cggagacctc ggagagacgt ggacgcgctt cgaccacggc gtgaaggccg agtccaccat
     1921 gatggcggtg gcgctgcatc cccgcgatgc cggccgcgtg tactgcgcga gccgtaccgg
     1981 gcagatcttc gcgacggagg acgcgggccg ctcgtggcga gaggagcggc tgcccgaggg
     2041 cgtgagcgac gtctacgcga tcgcctgcgg gtaaagcgcc ccctcaccct gccctctccc
     2101 ctctggggag agggagaggg gggcgaggtc gcggacgaag cagcggacgt cgcccaggtg
     2161 ctcgtagcgc tccgtgtgcg ccgggaggcc ggcgcgctcg ccgtcgatgc ccagggaccg
     2221 cagcaccggc tcggtgagga gcgcgtagcc gcccttccgt ccgaccccgt tcttgagcag
     2281 ccggtgcacc aggatcaccg cggtgccggt cacccggctg cgccccgcca ccgtgtgccg
     2341 caggaacggc ccgtggtgga cgaccatctt gagatcgagg gtgccgatct cctggcacgc
     2401 cccgcactgg cacttctcgg cggcctgcat cacctgctgc cggctcttga aggcggcgaa
     2461 cgcctcctcc agcaccgcca gcagccgggt gtgcggcagt gcgaagctct cgcccccgag
     2521 ggcgaacacc gcgtcgccct cgatctcctg gatctcgagc gggggcgaca ggtgcccgat
     2581 cacctcgtcg agcagggtcg cgatgatgtc cgagccatgc tcgagctcgg tggtggtcac
     2641 gaaggcggtg aatccggaga cgtcggcgag gacgaggaag ccgtgatccg ttcggttgtt
     2701 catgcccctg agacaccgct ccgggacatc cgtgaaacgc ccggatcgcc ggctaaaccc
     2761 gctccaggat gtgcagacct cggccccggt cgatgaggta gagcaggccg cgcccgtcca
     2821 cctccacgtc gttgctcgag acccgcgaat ggcccgccgg cggctccggc aggaaatggg
     2881 cgatctcacg tggctcatgc gggttggcga catcgacgat ccgaaggccg tgggcgaacc
     2941 aggccaccgg gatctcggtc ccggtcaccc gctcgcacgg ctggtggcag ccggtgaact
     3001 cgggctgcgg ctcgccgtcc accccggcca cctggaaggt cgagatgggg acagggtgcc
     3061 gctcgtctgt gatgtcgacc agccacatga acgagggggg ccccggcgcg gtcttggcca
     3121 cgtcctcgtc ggcggccagc atgatccgcc ggccgtggag cgggaacggc agcggcagcg
     3181 cggtgtgggt cggggtgatg tagggcgggc tccagtcgag cccggacacg aagcggggct
     3241 tggccatgtc ctcgatgtcg aggatgacga accccccgtg ccagtagctc acgtagagcc
     3301 ggttgccggc ccggatcggg tggtggcagc ggtggtgccg ccccgaccac gcgggctgct
     3361 ccccgccccc ggtccactgc cccggcatcc accagcggcc gacttccttc gggcgggtgg
     3421 ggtcgtggag atccaggatc atcacgatgt tgcccacgta gccatccatc tcgggggaga
     3481 tgtaagcgta gcgcccgtcg aaggtgaagc ggtgcacgcc ggccccgccg cacttccaga
     3541 acgtgatctc gcgcggccgc gccggggtgg agacgtcgta gatgacgagg cccccgccca
     3601 ccccggcggg cggctggtgc gcgggctgcg cctcgcggtt caccagcatg agcccgttgg
     3661 ccgcgcgcac cttgtgggaa tgggtgtgcg gcggcacctc gacggtggcc agggtgcgcg
     3721 gccgggccgg atcgctcacg tccacgatgc tggtgccgtg cggcgccttc atgtgggcga
     3781 cgtaggcgat ggtgccgtcg accgacacct gaccgccgcc cgggcagtcg agccaggcga
     3841 cctgcttgag tccgcgggtg ctcggggtga tcgtcgccat gtcgggcgga gcctagcaga
     3901 cgacgcgggc gccgccaagc ccttccgagc gccgatttga caggcgcccc ggggagtgac
     3961 agagtctcgg caactcgcaa aggagcgtcg ccatggccct ctccaccgat cggctcccgg
     4021 aagtcggcaa tgcctatgcg cggctcgagg acaagatcct cgagcgggac cagggcgcgg
     4081 ccagcgacat cttctacgat ctggtgcggg cgggggtgcc catcccccag ctggtcgggc
     4141 agctcgtgcg catccacgcg ccgtacaccc acgtgcccta tcaccagcgc ctcgatgacg
     4201 gcatcgtgcg cttcgtgaac aacgaccact gcctgctcag ctcgcgcgcg ggcatcgacc
     4261 tggtgccgct gatgccgccc gagctccggt acctgccctt cgcgcagacc atctggtaca
     4321 tgccgaccgg cctcgacccg tggaaccagc tcatcggcaa gatgcccggc cactacgtgc
     4381 ggctctacga gatcaagttc gagggcaagc cgccgctccc cgaggtgcac tggcccgacc
     4441 aggcgccgct cgccatcgag ggcacgtggc ccgagaagct caacacctgg ctcaccctcg
     4501 tgcagcgcgg cgaggtgatc aacgcctacc gggtcttcct cggcctgtgg gacgaagcgg
     4561 cggtcgatcc ggccaagcgg acgccgctgc tggcccagct cgtgttcgcg ggcctcatcg
     4621 acgtgcagga ccgcgtgctc tacaaccggt cctacaccac cggccacaag tcctatcgcg
     4681 cccgcgccac cgtcgagctg gcccgcgcgg tgggctggaa cgacgcgcac agcatcctct
     4741 acgcgggcgt gcccgacatc gcggtgggcc cgcggtggta ctcgacctac gagatggcct
     4801 gccagatcgt gcagatcttc ctcgacaacc gcgaccgcga gctgctcgac aacgacggcg
     4861 cgctcacccc ggtggaatcg cttgcgctgc agcaggcgct gctgagcggc caggagccgg
     4921 gccacatcga cctgatcgtg gcgctgctgc agaccgggcg cggcctgcgc cagatcatcg
     4981 acacgatcca gctcgcctcg gcccagctga tcatggagac cggcgatccc aacgcgtact
     5041 cgatgccgca gcacagctac gagtactgca acacggtgcg ctggttcttc gactccttcg
     5101 cgcacccgca tcggctcaag ctgctgttcg tggccgcgtc cttcgtcaac cgcgcggccc
     5161 atcaccagaa gcacaccccg gacaaccacc cggtgaaggt caccccgccc aagggctccg
     5221 acgcgctcac ccccgcgcag ctcctcgagc ggctggacag tgcgctcgac ggcctccgcg
     5281 cgttcgaggc gatggactgg acggcggcct acctgcgctc cggcgcggac cgcgccgcgc
     5341 tcgtgcagac gctggcggtg ggcgcggtca aggcgggcaa cgacccgcac aaccaggagc
     5401 tggggatctg cctgctccac gactacggcc actccaccgc ggccgaccgc gaccgcctgc
     5461 tgctcgcctc ggccaagcac accgccgggc accgaaagta cggcagccac ctcgacgcgt
     5521 ggcggcgcta cgcggacgcg ttcggcctcg cccgctcctg accgctgccg cgcgcgtcgg
     5581 ctagctcaga acgcgagtcc gttcgtgagg cgcagcgcct gctcgacgtg cccgcgcacc
     5641 ccgtccaggt acgccggctg ggcttgcccg ccctcctcct gatccagccg ggc
//
LOCUS       DATMCV010000928         6463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_184361, whole genome shotgun sequence.
ACCESSION   DATMCV010000928 DATMCV010000000
VERSION     DATMCV010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184361"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..2086)
                     /locus_tag="VNU03_21195"
     CDS             complement(59..2086)
                     /locus_tag="VNU03_21195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HWP77051.1"
                     /translation="MSTALAKPPTAEPKAAAPSATEMGARQRDISVSEFFTKNRHLLG
                     FDNPRKALLTCVKEAVDNALDASEEAGILPEVVVTLEVAPSNGDAPPPASQATRFRVT
                     VADNGPGIVRQQIPPIFAKLLYGSKFHRLRMSRGQQGIGISAAGMYAQLTTGKPVQII
                     SRTGARAAAHYFEVQIDTKKNEPRIFESKKIDWEQPRGTQVTLEIEGRYQKGRASVDE
                     FLEQVAIANPHVKLVYRTPEGETREYPRTIHELPPQPREIKPHPYGIEFGILLRMLHD
                     TRGHSLAGFLAGDFSRVSPAVAQEICKTAKLSPDARPRNVHGTAAEALYKAIQATKIM
                     APPSNCISPIGEKAILHGLYKQIKGEFYTAVTRPPAVYRGNPFAIEAGLAYGKGPDQV
                     AATREAPAVPLAEGEQRDDDDELARVIRYANRVPLLYQQSACATFKAAIETAWKNYGI
                     TQSRGALPAGPMVVFVHMASVWVPFTSESKEAIADYDEIRKEIKLALQECGRRLGVFL
                     RRRERAKSEFRRRNIFDLYIEEVVEACARLKGGRLPKEKLKTQLQRIALKRTGGTRTD
                     EILGRKSGPEGLPHSIIVTPEGTEGEAPDLPGDAADAAGVETSAAEPPETEVPTRRKG
                     KAKPARAAKPAPAAGREKSGRGPARKMTPAKATKGKTAKGKAAKAPKRRTR"
     gene            2229..2984
                     /locus_tag="VNU03_21200"
     CDS             2229..2984
                     /locus_tag="VNU03_21200"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HWP77052.1"
                     /translation="MRRKLPVSTASWIAVVALALMVAACAPTKVRSVSSYRGDESLPR
                     PDRVLVYDFAISPEQVSLNRGLFSRLGREVTSASATEAELQIGREVADGFSTELVKRI
                     QRLGLPAERAAADQVVPPGTLAITGQFLSIDEGNRMRRAVIGFGAGESEVKALAQVAL
                     QTAHGPLLAEEFETKAESARTPGVGPMVGPGIAVRGAAGVASSVAVSGGVHALAEHRT
                     EVEADARRIADGLGKQLGKFFVRHGWITEAEAR"
     gene            complement(3022..3918)
                     /locus_tag="VNU03_21205"
     CDS             complement(3022..3918)
                     /locus_tag="VNU03_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP77053.1"
                     /translation="MGLLDGKVAVVTGGGTGIGRAVSIGLAAAGAKVVVNDYGVSVDG
                     MDPSSEPANQVVADIRKAGGQAVAAADSVATMAGGRAVVDLALKEFGDLHVLVCCAGI
                     LRERMIFNMSEEDWDAVIAVHLKGHFTVMQPATRHMRERRAGSIITFTSTAGLEGSPG
                     QPNYSAAKEGIVGLTRSTALAMAKYGVRCNAISPTADTRMTQRLPGERRGAATATPPE
                     AIAPVATFLASDRAAHITGQVVGVRGSEVTVFSHPAPLRTVSAPGSWTPEQLADLWDR
                     TLGQDRLRRLDTMKIPWPPPTA"
     gene            4039..4716
                     /locus_tag="VNU03_21210"
     CDS             4039..4716
                     /locus_tag="VNU03_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77054.1"
                     /translation="MAKTRGTGLLMLWTDVDPQHEAEFNRWYDEEHLDRLLKVPGFLS
                     ASRYEAFRGGPKYLAMYELEEASVLRTAAFLDEVRFRPSARRQQSSGGHVGRNYLLNG
                     YRQIFPARTNPVELTMGPSPFLQMGRMDIPAHMEEEWNDWYNWVYVPDYLTVPGVIRA
                     RRFMAVEGQPKYLTVYEFERPDVPDTEAWNRVRDANPWTRRVRHHMRLDAGSPVVYKR
                     ITPRLGA"
     gene            4713..5045
                     /locus_tag="VNU03_21215"
     CDS             4713..5045
                     /locus_tag="VNU03_21215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HWP77055.1"
                     /translation="MIKRVSLLTRKDGMTREQFTRHWLEIHAPLARPVPGLRRYVQSH
                     ILEEFKRADIPTSAVQVDGIAELWFDDRPSMERALASPQMAALHADGALFIGANKTFV
                     VEEKVIIG"
     gene            5055..5660
                     /locus_tag="VNU03_21220"
     CDS             5055..5660
                     /locus_tag="VNU03_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated protein"
                     /protein_id="HWP77056.1"
                     /translation="MTAEPIALGVKIAYTLFLAVLVPVYWAHYGSKNFLWFSDIALLG
                     AGASVWLESPLLASMMMLAVLLPECAWNLDFFGRLLTSHRMFGMSAYMFEARTPRYLR
                     ALSLFHVPLPIGLVWLVHRLGYDRRAWLFQSLLALVVLPVTYRLTDPAENVNWVRGLG
                     SPQRSLPPWLYLTLLIVAFSLVLYLPPHLLFVYLPLSATSS"
     gene            complement(5630..>6463)
                     /locus_tag="VNU03_21225"
     CDS             complement(5630..>6463)
                     /locus_tag="VNU03_21225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77057.1"
                     /translation="ALKRGEVDIAYSIRGELAEEMQKTPGLTLKPAVIQSPFWLYFPD
                     QWDPKSPWHDRRVREAASLAIDRPSINQALTLGYSRLSGSIIPTSFEYFWQPPAPVYD
                     RARAAKLLAEAGYPNGFDAGEYYCDVSYANLGEAVVNSLQTVGIRAKLRPLERVAFFS
                     GYSDKKYRNIIQASSGAFGNAATRLEQFVATGGAYSYGGYPDTDGLMQEQAADADPKR
                     REATLRRIQQVVHEKVMYAPIWELAFLNGVGPRVRESGLGLIAGHAYSAPYEDVALKG
                     K"
BASE COUNT          834 a         2279 c         2298 g         1052 t
ORIGIN      
        1 gagcttcttc tcgaccacgc cggtcttgct cgcctttgcc ttggccatgg gctcgatcct
       61 accgcgttct gcgcttcggg gccttcgccg ccttgccctt cgcggtcttg cctttggtcg
      121 ctttcgcggg cgtcatcttc cgggctggcc cgcgccccga cttctcgcgg cccgccgcgg
      181 gcgcgggttt ggcggcccgg gccggcttgg ccttgccctt ccggcgcgtc gggacctcgg
      241 tctcgggcgg ctcggccgcg ctcgtctcca cgcccgcggc gtcggccgcg tcgccgggca
      301 ggtccggcgc ctccccctcc gtgccttccg gggtgacgat gatcgagtgc ggcaggcctt
      361 ccgggccgct cttgcggccc aggatctcgt cggtgcgggt gccgccggtg cgcttgagcg
      421 cgatccgctg gagctgggtc ttgagcttct ccttcggcag ccggccgccc ttgagccgcg
      481 cgcaggcctc gacgacctcc tcgatgtaga ggtcgaagat gttgcgccgg cggaactcgc
      541 tcttggcccg ctcgcgccgc cgcaggaaca ccccgagccg ccgcccgcac tcctgcagcg
      601 cgagcttgat ctccttccgg atctcgtcgt agtcggcgat cgcttccttg gactcgctcg
      661 tgaacgggac ccacaccgag gccatgtgga cgaacaccac catggggccc gcgggcaggg
      721 cgccgcggga ctgggtgatg ccgtagttct tccacgcggt ctcgatcgcg gccttgaagg
      781 tcgcgcacgc cgactgctgg tagaggagcg gcacccgatt ggcgtaccgg atcacgcgcg
      841 cgagctcgtc gtcgtcgtcg cgctgctcgc cctccgcgag agggaccgcg ggcgcctccc
      901 gcgtcgccgc gacctggtcc ggccccttgc cgtaggcgag ccccgcctcg atggcgaacg
      961 ggttcccgcg atacaccgcg ggcgggcgcg tcaccgcggt gtagaactcg cccttgatct
     1021 gcttgtagag gccgtggagg atcgccttct cgccgatcgg cgagatgcag ttcgagggcg
     1081 gcgccatgat cttggtcgcc tggatggcct tgtagagcgc ctccgcggcg gtgccgtgga
     1141 cgttgcgcgg ccgcgcgtcc ggcgagagct tggcggtctt gcagatctcc tgggccaccg
     1201 cgggcgagac ccggctgaag tcccccgcga ggaagccggc gagcgagtgg ccccgggtgt
     1261 cgtggagcat gcgcaggagg atgccgaact cgatgccgta cggatgcggc ttgatctcgc
     1321 ggggctgggg cggcagctcg tggatggtgc gcggatactc ccgggtctcg ccttccgggg
     1381 tgcggtagac cagcttcacg tgcgggttgg cgatggccac ctgctcgaga aactcgtcca
     1441 ccgaggcgcg gcccttctgg tagcggccct cgatctcgag agtgacctgg gtgccgcgcg
     1501 gctgctccca gtcgatcttc ttgctctcga agatccgcgg ctcgttcttc ttggtgtcga
     1561 tctgcacctc gaagtagtgg gccgccgccc gcgcgccggt gcgcgagatg atctggaccg
     1621 gcttgccggt cgtgagctgc gcgtacatgc ccgccgccga gatcccgatg ccctgctggc
     1681 cccggctcat gcgcaaccgg tggaacttcg agccgtagag gagcttggcg aagatgggcg
     1741 ggatctgctg gcggacgatg ccgggcccgt tgtcggccac cgtgacccgg aagcgcgtcg
     1801 cctggctggc cggcggcgga gcgtcgccgt tgctcggcgc cacttcgagg gtgacgacca
     1861 cctccggcag gatgcccgcc tcctcggagg cgtcgagggc gttgtcgacc gcctccttga
     1921 cgcaggtgag gagcgccttg cgcgggttgt cgaagccgag caggtggcgg ttcttggtga
     1981 agaactcgga gaccgagatg tcccgctggc gcgctcccat ctcggtcgcg gacggggcgg
     2041 cggccttcgg ctcggcggtc gggggtttgg ccagcgcggt ggacatctcc ctccttgata
     2101 ccgcaccccg cggtcgatag ggccaagaaa tcggggtccg gccacgccga tcgtgtagca
     2161 tccggggcac catgaccctg ccgagaggag tccccccgag aggaatcccc ccgagaggaa
     2221 tcccgaagat gcgccggaag ctccctgtct ccaccgcgag ctggatcgcg gtggtcgcgc
     2281 tcgcgctgat ggtggccgcc tgcgccccca ccaaggtgcg ctcggtgagc agctaccgcg
     2341 gcgacgagtc cctcccgcga cccgatcgcg tgctggtcta cgacttcgcg atctcccccg
     2401 agcaggtgag tctcaaccgc gggctcttct cgcgcctcgg ccgggaggtg acgagcgcgt
     2461 cggccaccga ggccgagctc cagatcgggc gcgaggtcgc ggacggcttc tcgaccgagc
     2521 tggtcaagcg gatccagcgc ctcgggctgc ccgccgagcg cgccgccgcc gatcaggtcg
     2581 tgccacccgg cacgctggcc atcaccggcc agttcctctc gatcgacgag ggcaatcgca
     2641 tgcggcgggc ggtgatcggc ttcggcgcgg gcgagagcga ggtcaaggcg ctcgcccagg
     2701 tcgcgctgca gaccgcccat gggcccctgc tggcggagga gttcgagacc aaggccgaga
     2761 gcgcgcggac cccgggggtc ggcccgatgg tcgggcccgg catcgcggtg cgaggggcgg
     2821 ccggcgtggc ctcgagcgtg gcggtgagcg gcggcgtgca cgcgctggcc gagcatcgca
     2881 ccgaggtcga ggcggacgcc cggcgcatcg cggacgggct cggcaagcag ctcgggaagt
     2941 tcttcgtccg gcacggctgg atcactgagg ccgaggcgag gtgagccgag ggcccggcgg
     3001 cccgcccctg gccgcgggtc ctcaggcggt gggcggcggc caggggatct tcatcgtgtc
     3061 gagccgccgc agccgatcct ggccgagcgt gcgatcccag agatccgcga gctgctcggg
     3121 cgtccagctg ccgggcgcgc tgaccgtccg cagcggcgcc gggtgcgaga acacggtgac
     3181 ctcgctgccg cgcacgccga cgacctgacc ggtgatgtgg gcggcgcggt cgctggccag
     3241 gaacgtggcg accggcgcga tggcctcggg cggggtggcg gtggcggcgc cgcggcgctc
     3301 gccgggcagg cgctgggtca tgcgggtgtc cgcggtgggc gagatcgcgt tgcagcgcac
     3361 cccgtacttc gccatggcca gcgcggtgga gcgggtgagg cccacgatgc cttccttggc
     3421 cgccgagtag ttcggctggc ccgggctacc ctcgaggccg gcggtggagg tgaaggtgat
     3481 gatgctgccc gcccggcgct cgcgcatgtg acgggtggcc ggctgcatca ccgtgaagtg
     3541 ccccttcagg tgcaccgcga tgaccgcgtc ccagtcctcc tcgctcatgt tgaagatcat
     3601 gcgctcgcgc aggatgcccg cgcagcacac cagcacgtgc aggtcgccga actccttcag
     3661 cgcgagatcc accaccgcgc ggccgcccgc catggtggcc acgctgtccg cggcggccac
     3721 cgcctgaccg cccgccttgc ggatgtcggc caccacctgg ttcgcgggct cgctcgaggg
     3781 atccatcccg tcgacgctga cgccgtagtc gttgaccacc accttggcgc ccgccgccgc
     3841 gaggccgatc gagaccgcgc gcccgatgcc cgtgccgccg cccgtcacca ccgccacctt
     3901 gccgtcgagc agtcccattt gacgtctctc ctcgccctgc cgtatcgtcg ccgcgttcac
     3961 gtcgaccgcc gccaatcctg ggagaggccg gccgtcgagt caagggcgcg gtccggccca
     4021 tccaaaggag gcgagcgaat ggcgaagacg cgcggcaccg ggctcttgat gctgtggacc
     4081 gacgtggacc cgcagcacga ggcggagttc aaccgctggt acgacgagga gcacctcgac
     4141 cggctgctga aggtgccggg cttcctgtcc gcgtcgcgct acgaggcctt ccgcggcggg
     4201 cccaagtacc tggcgatgta cgagctggag gaggcgagcg tgctccgcac cgcggccttc
     4261 ctcgacgagg tgcgctttcg cccctccgcg cgccgccagc agtcctccgg cgggcacgtg
     4321 ggccgcaact acctgctgaa cggctaccgt cagatcttcc ccgcgcgtac caacccggtc
     4381 gagctgacca tgggaccgtc gccgtttctc cagatggggc gcatggacat ccccgcgcac
     4441 atggaggagg agtggaacga ctggtacaac tgggtctacg tcccggacta cctcacggtg
     4501 ccgggggtga tccgcgcccg gcgcttcatg gcggtcgagg ggcagcccaa gtacctcacc
     4561 gtctacgagt tcgagcgacc cgacgtgccg gacaccgagg cgtggaaccg ggtgcgcgac
     4621 gccaacccgt ggacccggcg ggtgcgccat cacatgcgcc tcgacgcggg ctcgccggtg
     4681 gtctacaagc ggatcacccc gaggctcggc gcatgatcaa gcgcgtctcg ctgctgaccc
     4741 ggaaagacgg catgacccgc gagcagttca cgcggcactg gctcgagatc cacgccccgc
     4801 tcgcccggcc ggtcccgggc ctccggcgct acgtgcaatc gcacatcctg gaggagttca
     4861 agcgcgccga catcccgacc tcggcggtgc aggtggacgg tatcgccgag ctctggttcg
     4921 acgaccggcc gagcatggag cgcgcgctcg cctcgcctca gatggccgcg ctccacgccg
     4981 acggcgcgct cttcatcggg gccaacaaga ccttcgtggt ggaagagaag gtgatcatcg
     5041 gctaggcgac gccggtgacc gcggagccga tcgccctcgg ggtgaagatc gcctacaccc
     5101 tgttcctcgc ggtcctggtg ccggtgtact gggcgcacta cggctcgaag aacttcctgt
     5161 ggttctccga catcgcgctg ctcggggccg gggccagcgt gtggctcgag agcccgctgc
     5221 tcgcgagcat gatgatgctg gcggtgctgc tccccgagtg cgcctggaac ctcgatttct
     5281 tcggccgcct gctcaccagc caccggatgt tcggcatgag cgcctacatg ttcgaggcgc
     5341 gcaccccccg ctacctccgc gcgctctcgc tcttccacgt gccgctgccg atcgggctcg
     5401 tgtggctggt ccatcggctg ggctacgacc gccgcgcgtg gctcttccag agcctgctcg
     5461 ccctcgtggt gctgccggtc acctaccggc tcaccgatcc ggccgagaac gtgaactggg
     5521 tccgcgggct gggctccccg cagcggagcc tgcccccgtg gctctacctc acgctgctga
     5581 tcgtcgcgtt ctcgctcgtg ctctatctcc cgccgcacct gctcttcgtc tacttgccct
     5641 tgagcgccac gtcctcgtag ggtgcggagt aggcgtggcc cgcgatcagg ccgagccccg
     5701 actcccgcac ccgggggccg acgccgttga ggaaggccag ctcccagatc ggcgcgtaca
     5761 tcaccttctc gtggacgacc tgctggatgc gccggagcgt ggcctcgcgg cgcttcgggt
     5821 ccgcgtcggc ggcctgctcc tgcatcaggc cgtcggtgtc ggggtagccg ccgtaggagt
     5881 aggccccgcc ggtggccacg aactgctcga ggcgggtggc cgcgttgccg aacgcgccgc
     5941 tcgaggcctg gatgatgttc cggtacttct tgtccgagta gccgctgaag aacgcgacgc
     6001 gctcgagggg ccgcagcttc gcccggatgc ccaccgtctg cagcgagttg accaccgctt
     6061 cgcccagatt cgcgtaggac acatcgcagt agtactcgcc cgcgtcgaag ccgttggggt
     6121 acccggcctc ggccaggagc ttggccgcgc gcgcgcgatc gtagaccggc gcgggcggct
     6181 gccagaagta ctcgaacgag gtggggatga tgctcccgga gaggcgcgag tacccgaggg
     6241 tgagggcctg gttgatcgac ggccgatcga tggcgaggct cgcggcctcg cgcacgcgcc
     6301 ggtcgtgcca cggcgacttc ggatcccact ggtcggggaa gtagagccag aacggggact
     6361 ggatcaccgc gggcttgagc gtgaggccgg gcgtcttctg catctcctcg gccagctcgc
     6421 cgcggatcga gtatgcgatg tcgacctctc cgcgcttgag cgc
//
LOCUS       DATMCV010000929         2931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_713726, whole genome shotgun sequence.
ACCESSION   DATMCV010000929 DATMCV010000000
VERSION     DATMCV010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713726"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /gene="dapB"
                     /locus_tag="VNU03_21230"
     CDS             <1..415
                     /gene="dapB"
                     /locus_tag="VNU03_21230"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWP77058.1"
                     /translation="VAFKVLADMARMLGDDYDVEVVETHHRFKKDAPSGTALRMAEVV
                     AQALGRDLATAAVYDRHDRLAERTRKEIGMSALRSGDVVGEHTVSFASLGERLELTHR
                     AHSRDNFARGALRGARFIATARPGLYSMQDVLGLA"
     gene            412..1170
                     /locus_tag="VNU03_21235"
     CDS             412..1170
                     /locus_tag="VNU03_21235"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWP77059.1"
                     /translation="MRIVRFRAGGKIRYGVLEGTHILEYAGTPYGTFKKARKKYLLSQ
                     AVLLAPVVPSKIVAVGLNYRDHADEMNVQVPTEPPIFLKPVSALCGPDDPIVFPPQSR
                     RVDYEGELAVVMKKRCHLVTAERAREFVLGYTCLNDVTARDLHTRDGQPTRAKAFDSF
                     CPVGPCIATDIDPNGVDLETYVNGERRQASNTKYLICPVEDLVARISAIMTLLPGDII
                     ATGTPGGTGPLRPGDKVEVRIESIGSLKNPVIKI"
     gene            1182..2363
                     /locus_tag="VNU03_21240"
     CDS             1182..2363
                     /locus_tag="VNU03_21240"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HWP77060.1"
                     /translation="MSPSPSPIPLAGRLAKLPPYLFAEIDKNKREARARGVDLIDMGI
                     GDPDLPTPPHIIEAIKRAAETPANHRYPDYEGLLSFRTAVADWYRKRFGVTLSPESEV
                     LTLIGSKEGTAHMPLAFVNPGDVVLVPDPGYPVYAAGTWFAGGEVHFMPLRRANGFLP
                     DLDAIPADVAKRAKLMYLNYPNNPTAAVATREFFARAVEFARRNQLIVCHDAMYSELR
                     FDDHRPPSFLETPGAMEVGVEFHSLSKTYSMTGWRLGFCCGNAGALAGLGKIKTNVDS
                     GVFQAVQEAGIAALTGPQELSEQYRRTYQERRDVVVAGLKRLGWEVDVPKAAFFVWAP
                     VPGGLDSRAFAARLLNEVGCVITPGVGFGPSGEGFYRIALTIDAKRLQEAMDRLRGLK
                     L"
     gene            2375..2725
                     /gene="folB"
                     /locus_tag="VNU03_21245"
     CDS             2375..2725
                     /gene="folB"
                     /locus_tag="VNU03_21245"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWP77061.1"
                     /translation="MFLEDVRFYGHHGVTRAQQTVGAWFAVDAELMLDLAPAAASDDL
                     RATLDYGVIAARIVEVSTKERVNLLERLAGRLAQMLLAEFPCDEVRVRVRKLTPPMEG
                     LQGIPGVELTRRRR"
     gene            2732..>2931
                     /locus_tag="VNU03_21250"
     CDS             2732..>2931
                     /locus_tag="VNU03_21250"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWP77062.1"
                     /translation="MARVFLSVGSNLGDRLESLRRAVDRLRAMSDLRFLDASPLYRTE
                     PWEGAPGRKAEEWFFNCAIVIET"
BASE COUNT          450 a         1053 c          997 g          431 t
ORIGIN      
        1 cgtcgcgttc aaggtgctcg ccgacatggc ccggatgctg ggcgacgact acgacgtgga
       61 ggtcgtcgag acccaccacc ggttcaagaa ggacgcgccg agcggcaccg cgctgcgcat
      121 ggccgaggtg gtggcccagg ccctcgggcg tgatctcgcc accgccgcgg tttacgaccg
      181 gcacgaccgg ctcgcggagc ggacccggaa ggagatcggc atgtccgccc tccgctccgg
      241 ggacgtggtg ggcgagcaca ccgtgtcctt cgcctctctc ggcgagcggc tcgagctgac
      301 ccaccgcgcc cacagccggg acaacttcgc gcgcggcgcc ctgcgcgggg cccgcttcat
      361 cgccaccgcg cggcccggcc tctactccat gcaggacgtc ctcggcctcg catgaggatc
      421 gtccgcttcc gggccggcgg caagatccgc tacggagtcc tcgagggcac ccacatcctg
      481 gaatacgcgg ggacaccgta cgggaccttc aagaaggcgc ggaagaaata cctgctgtcc
      541 caggcggtcc tcctcgcccc ggtggtgccc tccaagatcg tggcggtggg cctcaactac
      601 cgcgaccacg cggacgagat gaacgtgcag gtgccgaccg agccgcccat cttcctcaag
      661 ccggtctccg ccctctgcgg gccggacgac ccgatcgtct tcccgcccca gtcgcggcgg
      721 gtggactacg agggagagct cgcggtggtc atgaagaagc gctgccacct ggtgaccgcg
      781 gagcgggcgc gcgagttcgt gctcggctac acgtgcctca acgacgtgac cgcccgcgat
      841 ctccacaccc gcgacggcca gcccacccgg gccaaggcct tcgacagctt ctgcccggtg
      901 gggccgtgca tcgccaccga catcgatccc aacggtgtcg acctcgagac ctacgtcaac
      961 ggcgagcgcc gccaggcctc caacaccaag tacctcatct gcccggtgga ggatctggtg
     1021 gcgcgcatct ccgcgatcat gaccctgctg ccgggggaca tcatcgccac cggcaccccg
     1081 ggaggcaccg ggccgctccg gccgggcgac aaggtcgagg tgcgcatcga gtccatcggc
     1141 agcctgaaga atcccgtcat caagatctag gagaaccacg cgtgagccca tcgccttcgc
     1201 cgatcccgct ggccggccgt ctcgccaagc tgcccccgta cctgttcgcc gagatcgata
     1261 agaacaagcg cgaggcgcgc gcccgcggcg tcgacctcat cgacatgggc atcggggatc
     1321 cagacctgcc gaccccgccc cacatcatcg aggcgatcaa gcgcgcggcc gagacccccg
     1381 cgaaccaccg ctaccccgac tacgagggct tgctgagctt ccgcaccgcg gtggccgact
     1441 ggtaccggaa gcgcttcggc gtcactctca gcccggagag cgaggtgctc accctcatcg
     1501 ggtcgaagga gggcaccgcg cacatgccgc tcgccttcgt gaacccgggc gacgtggtgc
     1561 tggtgccgga tccgggctac ccggtctacg ccgcgggcac gtggttcgcg ggcggcgagg
     1621 tgcacttcat gccgctccgg cgcgccaacg gtttcctgcc ggatctcgat gcgatcccgg
     1681 ccgacgtggc caagcgcgcc aagctcatgt acctcaacta cccgaacaat ccgaccgcgg
     1741 cggtggcgac cagggaattc ttcgcgcgag ccgtcgagtt cgcgcggcgg aaccagctca
     1801 tcgtgtgcca cgacgcgatg tactcggagc tgcgcttcga cgaccaccgg ccgccctcct
     1861 tcctggagac gccgggggcc atggaggtcg gggtcgagtt ccactcgctc tccaagacct
     1921 attccatgac cggctggcgt ctcggcttct gctgcggcaa cgcgggcgcg ctggccggcc
     1981 tggggaagat caagaccaac gtggactcgg gcgtgttcca ggcggtgcag gaggcgggca
     2041 tcgcggcgct gaccgggccc caggagctgt ccgagcagta ccggcgcacc taccaggagc
     2101 gacgcgacgt ggtggtggcc gggctcaagc ggctcggctg ggaggtcgac gtgcccaagg
     2161 ccgcgttctt cgtgtgggcc ccggtgccgg gcgggctcga ctcccgcgcc ttcgcggcgc
     2221 gcctgctgaa cgaggtcggg tgcgtgatca cccccggcgt cggcttcggg ccttccggcg
     2281 agggcttcta ccggatcgcg ctcaccatcg acgccaagcg cctgcaggag gccatggacc
     2341 gcctccgggg gctcaagctc tgacctctga taagatcttc ctcgaagacg tccgcttcta
     2401 cggccatcac ggcgtcaccc gggcgcagca gacggtgggc gcctggttcg cggtcgacgc
     2461 ggagctgatg ctggacctgg cgcccgccgc cgcgtcggac gatcttcggg ccacgctgga
     2521 ctacggagtg atcgccgcgc gcatcgtgga ggtctcgacg aaggagcggg tgaacctgct
     2581 ggagcggctg gccggacggc tcgcccagat gctgctcgcg gagttcccct gcgacgaggt
     2641 gcgggtgcgg gtccgcaagc tcacgccccc gatggagggg ctgcagggca tccccggcgt
     2701 ggagctgacc cgtcggcggc ggtagacaaa catggcgcgc gtgttcctgt ccgtcggatc
     2761 gaacctgggc gaccggctgg agtccctccg ccgcgcggtc gaccggctgc gcgccatgag
     2821 cgacctgcgg ttcctcgacg cctcgcccct ctaccgcacc gagccctggg agggcgcgcc
     2881 gggccggaaa gcggaggaat ggttcttcaa ctgcgcgatc gtgatcgaga c
//
LOCUS       DATMCV010000930         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_116723, whole genome shotgun sequence.
ACCESSION   DATMCV010000930 DATMCV010000000
VERSION     DATMCV010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116723"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..544)
                     /locus_tag="VNU03_21255"
     CDS             complement(314..544)
                     /locus_tag="VNU03_21255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77063.1"
                     /translation="MLDGILFEKIGVPAASIVTDVFESTGHAMAQSWGVPEYKFLAVP
                     HPIANLTDAELDARARAIAPEVVRLLLKGQER"
     gene            complement(566..856)
                     /locus_tag="VNU03_21260"
     CDS             complement(566..856)
                     /locus_tag="VNU03_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77064.1"
                     /translation="MMAGFEILDPTVEPRRQPLTYVPRPDSLKGKRVGLVENTKFNSD
                     KLLRKIGVILKTEYGAAEARMWRKHNSGVPAHKEILDDAKQAVDVVVAGIGD"
     gene            complement(853..1899)
                     /locus_tag="VNU03_21265"
     CDS             complement(853..1899)
                     /locus_tag="VNU03_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77065.1"
                     /translation="MTARISSQRYAVEDFQQALDLCYSRGWTDGLPVVPPTEPAVRAM
                     LDAVGLEPDTQVAFITNRQVAITAEKVAINAVMAGCLPEHMPVVLAALEGIADPRWGY
                     HGPATSTGSAAVLIIVNGPIAKRLDFNSGDNLFAPGWRSNATVGRAVRLVMRNVIGTM
                     PGRLDRGTFGHPGRYTYVMAENEAESPWTPLHVERLGCRPTDSAVTVMAALAPIQFYN
                     QLSSTAAGVLGTLCDTLRYPGQIGQPNYCVVLAGEHMRTIAADGWSKADIRKFIFENS
                     TNTVAHFKRTARMPGAVKPEDETANRPIVQSPDDILVVAAGGRAGSFSCFIPGWASRT
                     SSEAVTVVIKERSA"
     gene            2010..>2339
                     /locus_tag="VNU03_21270"
     CDS             2010..>2339
                     /locus_tag="VNU03_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP77066.1"
                     /translation="MGEREARPHVVIVGGGFGGLYAARALAERPVRVTLLDRRNHHLF
                     QPLLYQVATAALNPSDIATPLRSILRGASNVTVFLAEVESVDLAGRRLVLDEGPMTYD
                     ALVLAAGA"
BASE COUNT          309 a          857 c          775 g          398 t
ORIGIN      
        1 ccgcactccc ccactcggcg gacactggct tacttcctca agctcttcag ctgcgggggc
       61 ctcgacatgg cccccgcgct cccccactcg gcggacgctc gtttactgcc tcaaagccct
      121 tcagctgcgg gggcctcgac atggcccccg cactccccca ctcggcggac actcgtttac
      181 ttcctcaggc ccttcacctg cgggggcccc gacatggccc ccgcactccc ccactcggcg
      241 gacactcgtt tacttcctca ggccctttcg gcggacgctc gtttccgagt ctcgttcggt
      301 gctttctctg ggcctagcgt tcttggcctt tgagtagcag tcgcaccacc tcgggggcga
      361 tggcgcgggc tcgggcgtcg agctcggcgt cggtgaggtt ggcgatcggg tgcggaaccg
      421 cgaggaactt gtactccggg acgccccacg actgggccat ggcgtgaccg gtgctctcga
      481 agacgtcggt cacgatggat gcggcgggga cgccgatctt ctcgaacagg attccgtcga
      541 gcacactgcc cgacgagcac gaccctcagt cgccgatacc ggcgacgacg acgtcgaccg
      601 cctgcttggc gtcgtcgagg atctccttgt gggccggcac ccccgagttg tgcttgcgcc
      661 acatgcgcgc ttcggcggcg ccgtactccg tcttgaggat gaccccgatc ttccgcagca
      721 gcttgtccga gttgaacttg gtgttctcca ccagcccgac gcgcttgccc ttgagggaat
      781 ccgggcgcgg cacgtacgtg agcggctgtc gccgcggctc gaccgtgggg tcgaggatct
      841 cgaagcctgc catcatgcgc tcctctcttt gatgaccacc gtcactgctt ccgacgaggt
      901 gcggctcgcc cagcccggga tgaagcacga gaaggagccg gcgcgcccgc ccgccgccac
      961 caccaggatg tcgtccggcg actgcacgat cggccggttc gcggtctcgt cctccggctt
     1021 caccgcgccg ggcatccggg cggtccgctt gaagtgggcg accgtgttcg tcgagttctc
     1081 gaagatgaac ttgcggatgt ccgccttgga ccagccgtcg gccgcgatcg tgcgcatgtg
     1141 ctcgccggcc agcaccacgc agtagttggg ctgcccgatc tgccccgggt accgcagcgt
     1201 gtcgcacagc gtgccgagca cgccggccgc ggtgctcgag agctggttgt agaactggat
     1261 cggggccagc gcggccatca cggttaccgc gctgtcggtg ggccggcagc cgagccgctc
     1321 gacgtgcagc ggcgtccacg ggctctccgc ctcgttctcg gccatcacgt aggtataccg
     1381 gcctggatgg ccgaaagtcc cgcggtcgag gcggcccggc atcgtcccga tcacgttgcg
     1441 catcaccagg cgcaccgcgc gcccgacggt ggcgttcgac cgccagcccg gcgcgaagag
     1501 gttgtccccc gagttgaagt cgagccgctt cgcgatcggc ccgttcacga tgatgaggac
     1561 cgccgcgctc ccggtcgagg tggccggccc gtggtagccc cagcgcggat ccgcgatgcc
     1621 ctcgagcgcg gccagcacca ccggcatgtg ctcgggcagg cagcccgcca tcaccgcgtt
     1681 gatcgcgacc ttctcggcgg tgatcgccac ctgccggttc gtgatgaaag cgacctgggt
     1741 gtcgggctcg agcccgaccg catcgagcat cgcgcgcacc gccggctcgg tggggggaac
     1801 caccgggagc ccatcggtcc agccccggct gtagcagagg tcgagcgcct gctggaagtc
     1861 ctccacggcg tagcgctgtg acgagatccg cgcggtcatg agcgactcct cctgtgtggg
     1921 ctcaccctag ggcgaagccc gcgtcctgtc aagccgaccg cggctcccgc tcggttgaca
     1981 ccgtccccgg tcgcccggaa gatgaggcgg tgggcgagcg cgaggcgcgg ccgcacgtgg
     2041 tcatcgtcgg cggcgggttc ggcgggctct acgcggcccg cgcgctcgcg gagcggccgg
     2101 ttcgcgtcac cctgctcgac cgccgcaatc accacctctt ccagcccctc ctctaccagg
     2161 tggccaccgc ggccctcaat ccgtccgaca tcgcgacgcc gctgcgctcg atcctgcgcg
     2221 gggcctcgaa cgtcactgtc ttcctggccg aggtcgagtc ggtggacctg gccggccgcc
     2281 gcctcgtcct cgacgagggc ccgatgacct acgacgcgct cgtgctggcc gcgggggcg
//
LOCUS       DATMCV010000931         9808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_116728, whole genome shotgun sequence.
ACCESSION   DATMCV010000931 DATMCV010000000
VERSION     DATMCV010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116728"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VNU03_21275"
     CDS             complement(<1..599)
                     /locus_tag="VNU03_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77067.1"
                     /translation="MATAITERPTDPRRPSGHYRAYTPRPFTRAERDSVTVLFGGLHW
                     RVERILQAVLESAGHKAQVLPVATKDDLLTGREVADIGQCCPTSFTTGNLVNFLKKEA
                     KATSAAEVNQKFVYLTAGSCGACRFGQYHQSYELALRNSGLDAFRMFLLAQDQLDQKA
                     EMGDGLDLNLPVTLGCLWAIFCTDLVQDLEYQVRPFEVVP"
     gene            complement(580..1413)
                     /locus_tag="VNU03_21280"
     CDS             complement(580..1413)
                     /locus_tag="VNU03_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWP77068.1"
                     /translation="MPAFDLDTYVERSRAVDLSAIDWALVPRHPVPPETIRTLRFMQD
                     IESHTIIYLRALLATRAIDDPEVATFLACWVYEETFHGIALSRFLEAAGAPVGPRPTP
                     RGQESFGKRLEAAATAMVSKAWPDFCAVHMTWGAINELTTLTGYRRLALVSPHPVLND
                     LLDRIVLDESRHFFFYYRQAEMRMQGRAAARIARILVDRFWAPVGSGVQPDQELRFLA
                     TYLFAGPDGRAAARKVDDTIRRLPGFETVQLLEAWMDRHVENAHGSQPSGGGRNGDRD
                     H"
     gene            complement(1513..2139)
                     /locus_tag="VNU03_21285"
     CDS             complement(1513..2139)
                     /locus_tag="VNU03_21285"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWP77069.1"
                     /translation="MASDPEPPIVGQPAPLFSLTSVQGGTIDLATYRGRSNVVVWFSR
                     GFTCPFCRVYMDGMTEGYDGLREAGTEIIQVGPNLLQAARTFFGHSPTPYPFVCDPDK
                     RLFAVYGLGDRGALEATRSAVVSFSYAFTHGDTGPQLRGAWLDVMNRNFVRRLHHHAM
                     TALEQGIFLVDRSGVIRHRTVVGPTEPVPRGESLARMVHAYCPGTPGA"
     gene            2266..2973
                     /locus_tag="VNU03_21290"
     CDS             2266..2973
                     /locus_tag="VNU03_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77070.1"
                     /translation="MSQSSTIQIVYDFAFPDGGAKSYTVELDRATLSYLPGSQESPPA
                     WTRLENHQCGHCPLSPAEHPYCPVATNLSALVEFFKDKASVAEATVTVTSEERTYVKQ
                     LPLQRGIFGIFGLVMATSACPYMDFLKPMARFHLPFSTAEETIVRSVSMYLLRQYFVA
                     KRGGAPDLGLEQLETHYENIQKVNDGILERIKGVVLNDADLNALVILQGFADLLTMAI
                     AKDLSKIEPLFFRSEGA"
     gene            complement(3033..3965)
                     /locus_tag="VNU03_21295"
     CDS             complement(3033..3965)
                     /locus_tag="VNU03_21295"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP77071.1"
                     /translation="MKVGFIGLGTMGASMASNLQAGGHELQVSDVRKEAAAPHLKAGA
                     TWKDTPRQVAEGVEAVFTSLPGPKEVEQVALGPDGLIHGMKKGSAYFDLSTNSPALMR
                     RLYPAFQEKGIHVLDAPVSGGPKGAKSRRLALWIGGDREVYDRFKPVLDAIGDQPYYV
                     GPIGAGSVAKLVHNCAGYAVQTALAEVFTMGVKGGVDALTLWKAVRQGAGGRRRTFDG
                     LADQFLPGTFDPPAFALRLSHKDVSLAVGLARELGVPMRMANLVLEELTEAMNRGWGE
                     RDSRVAMLLQEERAGVKIAVPVEQLKEVLDKDGR"
     gene            complement(3962..4297)
                     /locus_tag="VNU03_21300"
     CDS             complement(3962..4297)
                     /locus_tag="VNU03_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP77072.1"
                     /translation="MTLPAAVSASRNEIRTVAPRLIELTETLVYPDIWERPGLSKRDR
                     SLITVAALTAMYRPDQLKGHTERALANGVTREEIGEIITHLAFYAGWPSAMSAAKIVK
                     QVFDQEKRA"
     gene            4377..5141
                     /locus_tag="VNU03_21305"
     CDS             4377..5141
                     /locus_tag="VNU03_21305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HWP77073.1"
                     /translation="MPNAPYRIYAVRYAHRKCTTSEAFYGDYHKGPMTMDYFVWALTN
                     GAETVVVDLGFTEAVGTRRGRQFLRDPGKGLAEIGVEAASVKHVILSHFHYDHVGNYA
                     LFPNATFYVQDSEMAFYTGRNASLPAFRHSVEIDDVCALIRLNYDRRMQFVDGSREIV
                     PGIAVHHVGGHTAGMQIVTVAHARGQAVVASDASHYYRNSEEGIPFNTLQDLPRMYSA
                     FARIRELASAPDLVLPGHDPLVLERLKRVGDGIVEM"
     gene            complement(5165..6025)
                     /locus_tag="VNU03_21310"
     CDS             complement(5165..6025)
                     /locus_tag="VNU03_21310"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWP77074.1"
                     /translation="MVWELVKRAAVKWNADDCLTMGAALAYYTVFSLAPILVIVIAVA
                     GAVFGPEAAQGEIVTQIRGLAGEEGATAIQSLIQSASRQGAGPRATIIGLLVLLFGST
                     SAFSQLQSALNTIWEVPPQAHAGLWAMIRARFLSFAAVLGTGFLLSVSLVLSAAVAAF
                     GRYGWGWVPGIGHALELADLLGSMLVHTLLFAMIFKLLPDVEIRWRDVWVGAAVTAGL
                     FFVGKVVIGVYLGSSDLGAAYGAASWVILILAWVYYSAQILLFGAEFTRAYQKLSPPH
                     PPLPSGERAG"
     gene            complement(6092..7726)
                     /locus_tag="VNU03_21315"
     CDS             complement(6092..7726)
                     /locus_tag="VNU03_21315"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HWP77075.1"
                     /translation="MRSSDLIARCLENEGVRYVFGVPGEEILDIVDSLADSSVRFVPT
                     RHEQGAAFMADAYGRLTGRAGVCLSTLGPGATNLVTGVADANLDHAPLVAITGQAGRD
                     RIHKESHQYLDIVEHMRPLTKWNTRAEMPVVIPEVIRKAFKVAEAEKPGACHVEVPED
                     VAEEPAEGEPLSTERPRRPSPDRQALQSAARLIEAASFPLIFAGNGVIRGNAAKELRE
                     LSRAHGIPVVNTFMAKGCMPYDDPLALLTAGLQSRDYVSCGFEKADLIIAVGYDLVEY
                     APKFWNPERKKHVIHIDFTPAEVDGYYQPAVEVVADVREALELLNGLVKGQKDPAPYR
                     ALRGYILEQLAEGADDDTFPIKPQRILRDLRAVMGREDILISDVGSHKLWIARTFPAY
                     EPNSVLISNGLAAMGFALPAAVAAKLVHPERNVVAVSGDGGFLMNVQELETAKRLGLA
                     IVNVIFRDGGYNLIQWKQQTRLGRESGVAFTNPDFVALARAFGATGFRVDSARDLRPV
                     LTEALACAGPSIVDVSVDYRENPKLTERLGHLVCPI"
     gene            7805..9169
                     /locus_tag="VNU03_21320"
     CDS             7805..9169
                     /locus_tag="VNU03_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWP77076.1"
                     /translation="MAIQSINPATGEVLETLEPTSPPQIQNILAGAHAAFLDWRTQPF
                     ARRSTLMRAAARELRTRKDEYALTMTREMGKPIVQAEAEVEKCAGTCDYYAEHAEAFL
                     AEQPRETDASRSYVRFDPLGVVLAVMPWNFPFWQVFRFAAPALMAGNAGILKHASNVP
                     RCALAIEEVFRRAGFPEGLFRTVLADSATVKGLIADPRIVAVTLTGSERAGQAVAQQA
                     GHELKKTVLELGGSDPFVVLADADVDAAAHAAAEGRLINSGQSCIAAKRFIVVEPIAD
                     RFLERFKGELASRRMGDPTARDTQVGPQARVDLRDELHRQVEESVKRGARALLGGQVP
                     SGKGAFYPPTLLAAVDRGMPAFDEETFGPVAAVIRAKDEADALRLANDSPFGLGAAIW
                     TKDRARAERLAAQIEAGAVFVNGVVKSDSRLPFGGIKRSGYGRELSEYGIREFVNIKS
                     VWIA"
     gene            complement(9282..>9808)
                     /locus_tag="VNU03_21325"
     CDS             complement(9282..>9808)
                     /locus_tag="VNU03_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77077.1"
                     /translation="TETIPYQVVRRGARRRAEHVMREGITVGLIGAAVAMLLFLGVDL
                     AAGAPLRTPAMLGNALFHGARGASEVAITTPVVLGYTAVHLAGFVLFGLAVSGLFALA
                     EREKRVLALIFMLGCCLAVVFVAMVYGISQWVSDAMTPWVFLAGHVLAGAAVVGGLVY
                     FHRRLLREFPTSAE"
BASE COUNT         1526 a         3458 c         3367 g         1457 t
ORIGIN      
        1 ggcaccacct cgaagggtcg cacctggtac tcgaggtcct gcaccaggtc ggtgcagaag
       61 atcgcccaca ggcagccgag ggtgaccggc aggttcagat cgaggccgtc gcccatctcc
      121 gccttctggt cgagctggtc ctgggcgagg aggaacatgc ggaaggcgtc gagcccggag
      181 ttgcgcaggg ccagctcgta gctctggtgg tactggccga agcggcacgc gccacaggag
      241 ccggcggtga ggtagacgaa cttctggttc acctccgcgg cgcttgtcgc cttcgcttcc
      301 ttcttgagga agttgaccag gttgccggtg gtgaagctgg tggggcagca ctggccgatg
      361 tcggcgacct cacggccggt gagcaggtcg tccttggtgg ccaccggcag gacctgggcc
      421 ttgtggccgg cgctctccag caccgcctgg aggattcgct ccactcgcca gtgcagcccg
      481 ccgaacagca cggtcaccga gtcgcgctcg gcgcgcgtga acggcctcgg ggtgtaggcg
      541 cggtagtgtc cgctcggccg gcgggggtcg gtgggtcgct cagtgatcgc ggtcgccatt
      601 gcgtcctcct cctgatggct gggagccgtg cgcgttctcc acgtggcggt ccatccacgc
      661 ctcgagaagc tgcacggtct cgaacccggg taagcgccgt atcgtatcgt cgaccttgcg
      721 ggccgcggcc cgtccatcgg gcccggcgaa cagataggtg gccaggaagc gcagctcctg
      781 gtcgggctgg acgccgctgc ccaccggcgc ccagaagcga tccaccagga tgcgcgcgat
      841 gcgggccgcg gcgcggccct gcatgcgcat ctccgcctgg cggtagtaga agaagaagtg
      901 gcgggactcg tcgaggacga tgcggtccag cagatcgttc aggaccgggt gtggcgacac
      961 gagcgccagc cgccggtagc cggtgagcgt ggtcagctcg ttgatggcgc cccacgtcat
     1021 gtgtaccgcg cagaagtcgg gccaggcctt cgacaccatg gcggtggccg ccgcctcgag
     1081 ccgcttgccg aacgactcct ggccgcgcgg tgtggggcgc ggaccgaccg gagcgcccgc
     1141 cgcctccagg aagcgcgaga gggcgatgcc gtggaacgtc tcctcgtaga cccagcaggc
     1201 caggaacgtg gccacctcgg ggtcgtcgat cgcccgcgtg gcgagcaggg ctcttagata
     1261 aatgatggtg tggctctcga tgtcctgcat gaagcggagc gtccggatcg tctcgggagg
     1321 gaccggatgc cggggcacca gggcccagtc gatggccgac aggtccacgg cccgcgagcg
     1381 ttccacatac gtatcgaggt cgaacgctgg catcagtcga gtcactacat acgacactgt
     1441 gggtggtatt tcaatgaccg tcccatgtcc tggcctgtgc ccttgtctac gatccggccg
     1501 ggtggcgcgg atctaggctc cgggagtgcc cgggcaatag gcgtggacca tccgggccag
     1561 gctctcgccc cggggcacgg gctccgtcgg tccgaccacg gtgcggtgac ggatcacccc
     1621 gctccggtcg accaggaaga tcccctgctc cagcgcggtc atcgcgtggt ggtggagccg
     1681 gcggacgaag ttgcgattca tcacgtcgag ccaggcgccc cggagctggg gcccggtgtc
     1741 tccgtgggtg aaggcgtagg agaagctcac caccgccgag cgcgtcgcct cgagcgcccc
     1801 gcggtcgccc aggccgtaca ccgcgaacag gcgcttgtcg gggtcgcaga cgaaggggta
     1861 cggggtcggg ctgtgcccga agaaggtgcg cgcggcctgg agtaggttgg gtccgacctg
     1921 gatgatctcg gtccccgcct cccgcaggcc gtcgtagccc tcggtcatgc cgtccatgta
     1981 gacccggcag aacgggcagg tgaagccccg ggagaaccag accacgacgt tggaccggcc
     2041 ccggtaggtc gcgaggtcga tggtcccgcc ctgcaccgag gtcaggctga agagcggcgc
     2101 cggctggccg acgatcggtg gctcggggtc ggacgccacg ggtctgagga tagcatgcgg
     2161 atcccgcagt tccggcacgt gacggataca ttgacgcgcc gccccgggct cgctacgctc
     2221 cccgggaaga gtggtcggtc cacccgcggc tcagaggggg taccggtgag ccagagcagc
     2281 accatccaga tcgtctacga cttcgcgttc cccgacggcg gcgccaagtc gtacaccgtc
     2341 gagctcgacc gcgccacgct ctcctacctg ccgggcagcc aggaatcgcc gccggcctgg
     2401 acccggctcg agaaccacca gtgcggccac tgcccgctca gccccgccga gcacccctac
     2461 tgcccggtgg ccacgaatct ctcggcgctg gtggagttct tcaaggacaa ggcctcggtg
     2521 gccgaggcca ccgtcaccgt cacctccgag gagcgaacct acgtcaagca gctccccctg
     2581 cagcgcggga tcttcggcat cttcggcctc gtcatggcca ccagcgcctg tccctacatg
     2641 gacttcctca agccgatggc ccgcttccac ctgccgttct ccaccgcgga ggagaccatc
     2701 gtgcgctcgg tctccatgta cctcctgcgc cagtacttcg tggccaagcg cggcggcgcg
     2761 cccgacctcg gcctcgagca gctcgagacg cactacgaga acatccagaa ggtgaacgac
     2821 ggcatcctcg agaggatcaa gggcgtggtc ctgaacgacg ccgacctgaa cgccctcgtc
     2881 atccttcagg gcttcgccga cctcctgacc atggcgatcg cgaaggatct ctcgaagatc
     2941 gagccgctct tttttaggtc ggagggggcc taacggcccc ctccgagagg cgccgccggc
     3001 gaagccggcg ctcgaaccaa gcgcgctgga ccttaccggc cgtctttgtc cagcacctcc
     3061 ttgagctgct cgacgggaac tgcgatcttg acgcccgccc gttcctcctg cagcagcatg
     3121 gccacccggg agtcgcgctc gccccagccg cggttcatcg cctcggtcag ctcttccagc
     3181 accagattgg ccatccgcat gggcacgccc agctcgcgcg cgaggccgac cgcgagcgag
     3241 acgtccttgt ggctcagccg cagcgcgaac gcgggaggat cgaaggtgcc gggaaggaac
     3301 tgatcggcga ggccgtcgaa ggtgcggcgc cgcccgcccg cgccctgccg caccgccttc
     3361 cagagggtga gcgcgtccac cccgcccttg acccccatcg tgaacacctc ggccagcgcg
     3421 gtctgcaccg cgtagcccgc gcagttgtgc acgagcttgg ccaccgagcc cgccccgatc
     3481 ggccccacgt agtagggctg gtcgccgatg gcgtcgagca ccggcttgaa gcgatcgtag
     3541 acctcgcggt ccccgccgat ccagagcgcg agcctgcgcg acttggcgcc cttgggcccg
     3601 ccgctcaccg gcgcgtcgag gacgtggatg cccttctcct ggaacgcggg gtagagccgc
     3661 cgcatcaggg ccggcgagtt ggtgctgagg tcgaagtagg ccgagccctt cttcatgccg
     3721 tggatgagcc cgtccgggcc gagcgcgacc tgctccactt ccttcggccc gggcagtgag
     3781 gtgaagaccg cctccacgcc ctccgcgacc tggcgcgggg tgtccttcca ggtcgcgccc
     3841 gccttcagat gcggcgcggc cgcttccttc ctcacgtcgc tcacctgcag ctcgtggccg
     3901 cccgcctgga gattggacgc catgctggcg cccatggtgc cgagaccgat gaacccgact
     3961 ttcatgcccg cttctcctgg tcgaagacct gtttgacgat cttggccgcc gacatcgcgc
     4021 tgggccagcc cgcgtagaag gccaggtggg tgatgatctc gccgatctct tcccgggtca
     4081 cgccgttggc gagggcccgc tcggtgtggc ccttgagctg gtcgggacgg tacatcgcgg
     4141 tcagggccgc caccgtgatg aggctgcgat cgcgcttgga gagaccgggg cgctcccaga
     4201 tatcgggata gacgagggtc tcggtcagct cgatgagcct cggggcgacc gtgcggatct
     4261 cgttgcgaga cgccgatact gcggctggta gggtcatgcg aactcctcct ctcgagtggg
     4321 acttccgtca acactggaaa cgcgtagaat cctgccacgg gaaaggagac ttgaccatgc
     4381 ccaatgcgcc ctaccgcatc tacgccgtgc gctacgccca ccgcaagtgc accacctccg
     4441 aggccttcta cggcgactac cacaagggcc ccatgacgat ggactacttt gtgtgggcgc
     4501 tcaccaacgg agccgagacg gtggtggtgg acctcgggtt caccgaggcg gtcggcacgc
     4561 ggcgcggtcg ccagttcctg cgcgacccgg gcaagggcct ggccgagatc ggggtggagg
     4621 cggcctccgt caagcacgtg atcctgagcc acttccacta cgaccacgtg ggcaactacg
     4681 cgctcttccc caacgcgacc ttctacgtgc aggactccga gatggcgttc tacaccggcc
     4741 gcaacgcctc cctgcccgcc ttccgccact cggtggagat cgacgacgtc tgcgcgctga
     4801 tccgcctcaa ctacgaccgg cgcatgcagt tcgtggacgg ctcacgcgag atcgtgcccg
     4861 gcatcgcggt gcaccacgtg ggcggccata ccgcgggcat gcagatcgtg accgtcgccc
     4921 acgcgcgcgg ccaggccgtc gtcgcctccg acgcctcgca ctactaccgc aattccgagg
     4981 aaggcatccc cttcaacacg ctccaggacc ttccgcgcat gtactcggcc ttcgccagga
     5041 tccgcgagct ggcgtcggct ccggacctgg tgctgcccgg gcacgatccg ctcgtgctcg
     5101 agcggctcaa gcgcgtgggg gacgggatcg tggagatgta gggggcgcgt cgcaagacca
     5161 cccctcaccc tgccctctcc cctgagggga gagggggatg aggtggggac aacttttgat
     5221 aggcgcgggt gaactcggcg ccgaagagga ggatctgggc cgagtagtag acccaggcca
     5281 ggatgaggat gacccagctc gcggccccgt aagccgcgcc gaggtcgctg ctgccgaggt
     5341 acacgccgat caccaccttg ccgacgaaga agagccccgc ggtgaccgcg gcccccaccc
     5401 acacgtcgcg ccaccggatc tccacgtcgg gcagcagctt gaagatcatc gcgaagagca
     5461 gggtgtgcac gagcatcgag cccagcaggt cggccagctc gagcgcgtgg ccgatgcccg
     5521 gcacccagcc ccacccgtag cggccgaacg cggcgaccgc cgcgctcagc accagggaga
     5581 cgctcagcag gaagccggtc ccgagcaccg ccgcgaacga caggaagcgg gcccggatca
     5641 tggcccagag ccccgcgtgg gcctgcggcg gcacttccca gatggtgttc agcgccgact
     5701 ggagctggct gaacgcgctc gtggacccga agagcagcac cagcaggccg atgatggtgg
     5761 cgcgcgggcc ggcgccctgg cggctcgcgc tctggatgag ggactggatg gcggtggcgc
     5821 cctcctctcc cgccagcccg cggatctggg tgacgatctc accctgggcg gcctccggcc
     5881 cgaacaccgc gccggccacc gcgatcacga tgaccaggat gggggccagc gagaagacgg
     5941 tgtagtaggc gagcgccgcc cccatcgtca ggcagtcgtc cgcgttccac ttgaccgcgg
     6001 cccgcttgac cagctcccag accattccca cgatccagga cgcagcaagg cgcgtgccgt
     6061 cagcgccggg aaggtcagct cggcaatccg gtcagatcgg gcagaccagg tggccgaggc
     6121 gctcggtcag cttcgggttc tcccggtagt ccaccgacac gtccacgatg gaagggccgg
     6181 cacaggcgag cgcctcggtc agcaccggcc gcaggtcccg cgccgagtcc acccggaagc
     6241 cggtcgcccc gaaggcgcgg gcgagcgcca cgaagtccgg gttggtgaag gccacgcccg
     6301 actcgcggcc gaggcgcgtc tgctgcttcc actggatgag gttgtagccg ccgtcgcgga
     6361 agatcacgtt gacgatggcc aggccgaggc gcttcgcggt ctccagctcc tggacgttca
     6421 tcaggaagcc accgtcgccg ctcaccgcca ccacgttgcg ctcggggtgc acgagcttgg
     6481 ccgccactgc ggcggggagc gcgaacccca tcgcggcgag gccgttggag atcagcacgc
     6541 tgttcggctc gtaggcggga aaggtgcgcg cgatccagag cttgtggctg cccacgtccg
     6601 agatcaggat gtcctcgcgg cccatcaccg cccgcaggtc gcgcaggatg cgctgcggct
     6661 tgatcgggaa cgtgtcgtcg tccgcaccct ccgcgagctg ctcgaggatg tagccgcgca
     6721 gcgcccggta cggcgcgggg tccttctggc ccttcaccag gccgttgagc agctcgaggg
     6781 cctcgcgcac gtcggccacc acctcgaccg cgggctgata gtagccgtcg acctcggcgg
     6841 gggtgaagtc gatatggatg acgtgcttct tgcgctccgg gttccagaac ttgggcgcgt
     6901 actcgaccag gtcgtagccc accgcgatga tcaggtccgc cttctcgaac ccgcacgaga
     6961 cgtagtcgcg cgactgcagc ccggcggtga gcagcgcgag cgggtcgtcg tagggcatgc
     7021 accccttggc catgaaggtg ttgaccaccg ggatgccgtg ggcccgcgac agctcgcgca
     7081 gctccttggc cgcgttgccg cggatcaccc cgttgcccgc gaagatgagc gggaacgacg
     7141 cggcctcgat gaggcgcgcg gccgactgca gggcctggcg gtccggcgac ggccgccgcg
     7201 gccgctcggt cgacaggggc tcgccctcgg ccggctcctc cgccacgtcc tcgggcacct
     7261 cgacgtggca ggcgcccggc ttctcggcct ccgcgacctt gaaggccttg cggatgacct
     7321 cggggatcac caccggcatc tcggcccgag tgttccactt ggtgagcggg cgcatgtgct
     7381 cgacgatgtc gagatactgg tgcgattcct tgtggatgcg gtcgcgcccg gcctggccgg
     7441 tgatggccac cagcggcgcg tggtcgaggt tggcgtcggc caccccggtg accaggttgg
     7501 tggccccggg cccgagggtg gacaggcaca cccccgcgcg gccggtgagc cgcccgtagg
     7561 cgtcggccat gaaggcggcg ccctgctcgt gccgggtcgg cacgaagcgc acgctcgagt
     7621 cggcgagcga gtccacgatg tcgaggatct cctcgccggg caccccgaac acgtagcgca
     7681 ccccttcgtt ctcgagacag cgggcgatca gatcggacga tctcatggcg ttcccccggc
     7741 tcagtctacg ccgatttgcg ccgcgtcaaa acggagtcca tgctgatggc cagaggagga
     7801 cgccatggcc atccaatcca tcaatccggc aaccggagaa gtcctcgaga ccctcgagcc
     7861 cacctctccg ccgcaaatcc agaacatcct ggccggcgcg cacgccgcat tcctcgactg
     7921 gcgcacccag cccttcgccc ggcgctccac cctcatgcgc gcggcggcgc gagagctgcg
     7981 gacgcgcaag gacgagtacg cgctcaccat gacccgtgag atgggcaagc cgatcgtgca
     8041 ggcggaagcc gaggtggaga agtgcgccgg cacctgcgac tactacgcgg agcacgcgga
     8101 ggccttcctc gccgagcagc cacgcgagac cgacgcctcc cgcagctacg tccgcttcga
     8161 ccctctcggg gtcgtgctgg cggtgatgcc gtggaacttt cccttctggc aggtgttccg
     8221 ctttgcggct cccgcgctca tggcgggcaa cgcgggcatc ctcaagcacg cctccaacgt
     8281 gccgcgctgc gcgctcgcca tcgaggaggt gttccgccgc gccggcttcc cggaggggct
     8341 gtttcgcacc gtgctggccg actccgccac cgtcaagggc ctgatcgccg atccgcgcat
     8401 cgtcgcggtc accctgaccg gcagcgagcg cgcgggccag gcggtggccc agcaggcggg
     8461 ccacgagctc aagaagacgg tcctggagct cggcggcagc gacccgttcg tcgtgctggc
     8521 cgacgcggac gtggacgcgg cggcgcacgc agccgcggag ggccggctga tcaacagcgg
     8581 gcagagctgc atcgcggcga agcgcttcat cgtggtggag cccatcgcgg atcgcttcct
     8641 ggagcggttc aagggcgagc tggcgtcgcg ccgcatgggc gaccccaccg cgcgcgacac
     8701 gcaggtcggc ccgcaggccc gcgtcgacct gcgcgacgag ctgcaccgcc aggtcgagga
     8761 gtcggtgaag cgcggcgccc gcgcgctgct cggcgggcag gtcccgagcg gcaagggcgc
     8821 gttctacccg ccgaccttgc tggccgcggt ggatcgcggc atgcccgcct tcgacgagga
     8881 gacgttcggc cccgtcgcgg cggtgatccg ggccaaggac gaggcggacg cgctccggct
     8941 cgccaacgac tcaccgttcg gcctgggggc cgcgatctgg acgaaggacc gcgcccgtgc
     9001 tgagcgactg gccgcgcaga tcgaggcggg cgcggtgttc gtgaacgggg tggtgaaatc
     9061 ggactcccgc ctccccttcg gcggcatcaa gcgctccggc tacggccgcg agctgtccga
     9121 gtacgggatc cgcgagttcg tcaacatcaa gtcggtctgg atcgcgtgac cggccatcgg
     9181 gtcggatcta cccggtagcg actaccgcgg cgggggcccc gacggccccc gccgcccgca
     9241 ctgctactcg gcccccgtgc tgctactcgg cccccgcact gctactcggc tgaggtcggg
     9301 aactcgcgga gcagccggcg gtggaagtac acgagcccgc ccaccaccgc ggcgccggcc
     9361 aggacgtgcc cggccaggaa cacccacggc gtcatcgcgt cgctcaccca ctgcgagatc
     9421 ccgtagacca tggccacgaa gacgacggcc aggcagcagc ccagcatgaa gatcagcgcg
     9481 agcacgcgct tctcgcgctc ggccagggcg aagagcccgg agaccgcgag cccgaacagg
     9541 acgaagcccg cgaggtgcac cgcggtatag ccgagcacca ccggcgtggt gatcgccacc
     9601 tcgctcgcgc cccgcgcgcc gtggaacaac gcgttgccga gcatcgcggg ggtgcgcaga
     9661 ggtgcccccg cggccaggtc gactcccagg aagagcagca tcgcgaccgc ggccccgatg
     9721 agccccaccg tgatgccttc gcgcatgacg tgctccgcgc gtcgcctcgc gccgcgccgt
     9781 accacctgat acggaatcgt ctccgtcg
//
LOCUS       DATMCV010000932         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_585241, whole genome shotgun sequence.
ACCESSION   DATMCV010000932 DATMCV010000000
VERSION     DATMCV010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585241"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VNU03_21330"
     CDS             <1..238
                     /locus_tag="VNU03_21330"
                     /inference="COORDINATES: protein motif:HMM:NF015589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HWP77078.1"
                     /translation="RLRGAVLHLDPCIPRAWRRFEITFRYHASRYEIEVLNPNGATRG
                     ISTIDLDGIRQSVASGSIPLTSDGATHRIRVVLG"
     gene            complement(258..710)
                     /locus_tag="VNU03_21335"
     CDS             complement(258..710)
                     /locus_tag="VNU03_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HWP77079.1"
                     /translation="MRIWVDADACPQVIKEILYRAAERVQVLTTLVANSALRIPPSDF
                     IRTVRVSQGSDVADHRIVQEVEPGDLVVTADIPLAAAVIARGAHALDPRGELYSEDNV
                     RERLAIRNLMQDLRATGTLIDGPAPFGPRERRLFADHLDHLLTRRPPA"
     gene            complement(799..999)
                     /locus_tag="VNU03_21340"
     CDS             complement(799..999)
                     /locus_tag="VNU03_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWP77080.1"
                     /translation="MAQGTVKWFNDAKGYGFITQEAGEDVFVHFNAIQGTGFKSLSEG
                     DKVEFEVTKGPKGLQAANVRKA"
     gene            complement(1190..1963)
                     /locus_tag="VNU03_21345"
     CDS             complement(1190..1963)
                     /locus_tag="VNU03_21345"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWP77081.1"
                     /translation="MTMSSAIWMVPLVALVLTQIAAIATSVYLHRGLAHRSLRLHPVA
                     DAAFRTVLWLTTGQTRREWVAVHRKHHTFTDREGDPHSPRLLGLWRVQAWNVYYYMRE
                     ARNAETIRTFAPDIAEDWGDRVFFSRGLSGLGLGIALLCLLLGLWPGLLTGLVHAILY
                     VFVVAPLINGLGHWRGAQNFANTAYNSRVLAWLTGGESLHNNHHAHPRAPKFSMRRLE
                     FDPSWVVIRALAAVGLVEVLGAPVGLGPHHSLDKSGAGL"
     gene            complement(1966..2196)
                     /locus_tag="VNU03_21350"
     CDS             complement(1966..2196)
                     /locus_tag="VNU03_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77082.1"
                     /translation="MAELEERLRGELSAVLDRIRGAGGGVMFEEFPGAIEDLGGSDPA
                     EDDRSATRRALIEQANDLAETLERIRHGEGGM"
     gene            2477..>2629
                     /locus_tag="VNU03_21355"
     CDS             2477..>2629
                     /locus_tag="VNU03_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWP77083.1"
                     /translation="MFGRSAPMQEVHRLIEQAAATPAPVLIHGETGTGKELVARTLHE
                     LSARASG"
BASE COUNT          408 a          954 c          872 g          395 t
ORIGIN      
        1 ccggctccgg ggggccgtgc tccatctcga cccctgcatc ccgcgcgcgt ggcgtcgctt
       61 cgagatcacg ttccggtacc acgcgtcgcg ctacgagatc gaggtcctga acccgaacgg
      121 ggccacccgc ggcatctcga ccatcgacct cgacgggatt cgccagtcgg tcgcgagcgg
      181 cagcatcccg ctcacgagcg acggcgccac ccaccggatc cgcgtcgtcc tcgggtgagg
      241 gggcggcccg cggcgcccta ggcgggcggg cgccgggtca gcaggtggtc gaggtgatcg
      301 gcgaagaggc gccgctcgcg cgggccgaac ggggcgggcc cgtcgatgag cgtgccggtc
      361 gctcgcaggt cctgcatcag gttgcgaatg gcgagtcgct cgcggacatt gtcctccgag
      421 tagagctcgc cgcgcgggtc gagcgcgtgg gcgccccgcg cgatgaccgc ggcggccagc
      481 gggatgtcgg cggtgaccac gaggtctccc ggctccacct cctgcacgat ccggtgatcc
      541 gccacgtcgg agccctgcga cacccgcacg gtgcggatga agtcggacgg cgggatgcgc
      601 agcgcgctgt tggcgaccag cgtggtcagg acctgcaccc gctcggcggc ccggtagagg
      661 atctccttga tgacctgggg gcacgcgtcg gcgtctaccc agatccgcac gcgcttcccc
      721 ccggagtgac gaaggaatga aagagaacgg ccgggccccg aaaggggacc cggccgtcgt
      781 ttgatacgtg gggtgtgctc aggccttccg aacgttggcg gcctggagcc ccttcgggcc
      841 cttggtcacc tcgaattcga ccttgtcacc ctcggacagg ctcttgaagc ccgtcccctg
      901 gatggcgttg aagtgcacga acacgtcctc gccagcctcc tgggtgatga agccgtagcc
      961 cttcgcatcg ttgaaccact tgaccgtacc ctgagccatt gacactacct ctcttgcgag
     1021 gcttgcgcct cggtgaaacc ccgccaccat ggcgggggga tcctcctgaa gtggaggaga
     1081 aacaaaaacg ccgccggact tacggtccgc gcggcgtttg atctcgactc tcgcgactct
     1141 gtgttcgcaa ccaatcggca accgagggaa cctaccactg gagacgaggc tacaggccgg
     1201 ccccactctt gtcaagggaa tgatgggggc cgagcccgac cggggctccc aggacctcca
     1261 ccagcccgac cgcggcgagg gcccggatca ccacccagga cgggtcgaac tcgagccgcc
     1321 gcatgctgaa cttcggggcc cgcgggtgcg cgtgatgatt gttgtggagg ctctcgcccc
     1381 cggtcagcca cgccagcact cgcgagttgt aggcggtgtt ggcgaagttc tgcgctcctc
     1441 gccagtgccc gagcccgttg atcaggggcg cgaccacgaa cacgtacagg atcgcgtgca
     1501 ccagcccggt gagcagtccc ggccagaggc cgagcaggag gcacaggagc gcgatcccca
     1561 gcccgagccc gctcaggccc cgggagaaga agacgcgatc gccccagtcc tccgcgatgt
     1621 cgggcgcgaa ggtccggatc gtctccgcgt tgcgcgcctc ccgcatgtag tagtagacgt
     1681 tccaggcctg gacccgccag aggccgagga gccgggggct gtggggatca ccctcgcgat
     1741 ccgtgaacgt gtggtgcttg cggtgcaccg cgacccactc ccggcgcgtc tgccccgtcg
     1801 tgagccacag caccgtccgg aacgcggcgt ccgccaccgg atgcagccgt agcgagcggt
     1861 gcgcgagtcc ccggtgcagg tagaccgacg tcgcgatggc cgcgatctgg gtcaggacca
     1921 gcgccacgag gggaaccatc cagatcgcag acgacatcgt catcctcaca tccctccctc
     1981 gccgtgccga atccgctcca gcgtctcggc gagatcgttg gcctgctcga tcagcgcccg
     2041 gcgagtggcc gagcggtcgt cctccgcggg gtcactcccg ccgagatcct cgatcgcgcc
     2101 gggaaactcc tcgaacatca cgcctccacc cgcccctcgg atccggtcca gcaccgcgct
     2161 cagctctccg cgcagtcgct cttcgagttc ggccatcgtc cgcctccatg cccacaccct
     2221 gtgttgtcgc cctcacggta tcaggcgggt gtccgggccg cctattggac cttggtccaa
     2281 gcggcgcgcc ccgtgacacc ggggctggac caaggtccac taggcgggac ggcgacccgc
     2341 ctgtaccgtg gagcaagact cgggcggacc gctctcgcat tccgtgaggc tcggcctctc
     2401 gccccgaaag gcgtcgatgc ttcttgccca acgagctgcc gtcgtgaacg gagcgccccg
     2461 cggcatcggt cgtcccatgt tcggacgcag cgcgcccatg caggaggtcc accggctcat
     2521 cgagcaggcc gcggccaccc ccgcccccgt cctcatccac ggcgagaccg gcaccggcaa
     2581 ggagctggtc gcccgcaccc tccacgagct ctccgcccgc gccagcggc
//
LOCUS       DATMCV010000933         3564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_987247, whole genome shotgun sequence.
ACCESSION   DATMCV010000933 DATMCV010000000
VERSION     DATMCV010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987247"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VNU03_21360"
     CDS             <1..136
                     /locus_tag="VNU03_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWP77084.1"
                     /translation="LAVPIKLVGVGEQIEDLQPFEPGTFVDALLGEEPGEQPETTPAG
                     "
     gene            177..1271
                     /gene="ribD"
                     /locus_tag="VNU03_21365"
     CDS             177..1271
                     /gene="ribD"
                     /locus_tag="VNU03_21365"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWP77085.1"
                     /translation="MDDIRWMTRALALAARGRGLTSPNPMVGALVVRDGAVIAERFHE
                     RAGGPHAEAAALEEAGARARGATLYVTLEPCNHVGRTPPCVEAIVRAGVRRVVAATPD
                     PNPRVRGGGAAALAAASIEVDLGCLEREARELNHAFFTATSLQRPHVTAKWAMTLDGK
                     IAAHDRQSRWITGEAARREAHRLRSEADAIVTGIGTVLADNPALTVRLEQPWPREPYR
                     VVVDGRARLPLEAAMLRAGTRARILVAVGEDAPAARVAGLASSGVTVLQCKSRDGRVD
                     VVDLSARLFALDVIAVLLEAGAELTGAFVQAGLVDRVAAFVAPKVLGGSAAPTPAGGP
                     GLRLGDALRLSDLTARPLGEDWLLEGRVER"
     gene            1273..1902
                     /locus_tag="VNU03_21370"
     CDS             1273..1902
                     /locus_tag="VNU03_21370"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWP77086.1"
                     /translation="MFTGLVEEVGRVLERAGSRLVLGADRVLEDSGVGASVAVNGACL
                     TVVEVGAGRLRFDVGPETLARTALGDLAVGDPVNLERPMRLGGLVGGHLIQGHVDGVG
                     TVAALTRQAETARLTIEWPGEALAPLLIPQGSVAVDGVSLTVARLNERDFEIMVIPHT
                     LAATTLGGLTRGRRVNLEMDMIGKYVQRVLQLREPPAPRKGPDAREGTR"
     gene            1899..3116
                     /locus_tag="VNU03_21375"
     CDS             1899..3116
                     /locus_tag="VNU03_21375"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWP77087.1"
                     /translation="MSESEFVPIEEGIEEIRAGRMLIVVDDEDRENEGDLLMAAEKAT
                     PEAVNFMAKYGRGLICMPMLGERLDELQISMMVSDNTAPLGTAFTVSVDARRGVTTGT
                     SAYDRAVTIRTLVDATTRPEDLTRPGHIFPLRAMPGGVLRRAGHTEAAIDLARLAGCA
                     PAGVICEVLDDEGGMARLPELMALSRQYRLKVITIKALIEYRIHKEKLVRRAATTRMP
                     TEYGEFQVIVYETTVDERQPLAMVLGDVKTDDPVLVRVHSECLTGDVFGSLRCDCGPQ
                     LHKALAIIQHAGRGILVYMRQEGRGIGLLNKLKAYELQDAGKDTVEANHALGFRADLR
                     HYGIGAQILVDLGVKNLKILTNNPKKIVGIEGYGLKVVERLPLEVPATDANRSYLRTK
                     REKLGHLFSSFPD"
     gene            3120..>3564
                     /gene="ribH"
                     /locus_tag="VNU03_21380"
     CDS             3120..>3564
                     /gene="ribH"
                     /locus_tag="VNU03_21380"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWP77088.1"
                     /translation="MAGIAPKRSVAPAARGQRFAVVVARFNEPISKRLLDGALGALAD
                     HAVTGDLVEVHWVPGSFELGQAAMTLARGGRFAGIVCVGVVIKGATPHFDYVCSAAAH
                     GIARAGLDTGVPVTFGVITALTEEQAWERAGGAVGNRGEEAALAAL"
BASE COUNT          495 a         1260 c         1347 g          462 t
ORIGIN      
        1 gctggcggtg ccgatcaagc tggtcggggt gggtgagcag atcgaggatc tccagccctt
       61 cgagcccggg accttcgtgg acgccctcct cggggaggag ccgggcgagc agccggagac
      121 cacgccggca gggtagaatg cgtgccgggg cggcttcgga gcgccccggc gaacccatgg
      181 acgacatccg gtggatgacc cgcgcgctgg ccctggccgc tcgcggccgg gggctcacgt
      241 cccccaatcc gatggtcggc gccctggtgg tccgggacgg cgcggtgatc gccgagcggt
      301 tccacgagcg ggccggcggc ccgcacgcgg aagcagccgc gctcgaggag gccggggcgc
      361 gggcccgggg cgccaccctc tacgtcaccc tcgagccgtg caatcacgtg ggccggaccc
      421 cgccctgcgt cgaggccatc gtccgcgcgg gcgtgcgccg ggtcgtcgcg gccacgcccg
      481 atccgaaccc gcgcgtgagg ggcgggggcg cggcggcgct ggccgccgcc tccatcgagg
      541 tcgacctggg ctgcctcgag cgggaagcgc gggagctgaa ccacgcgttc ttcaccgcga
      601 cctcgctcca gcgcccccac gtgaccgcga agtgggccat gaccctcgac ggcaagatcg
      661 cggcccacga tcggcaatcg cggtggatca ccggagaggc ggcgcgccgg gaggcccatc
      721 gcctgcgcag cgaggccgac gccatcgtca ccgggatcgg caccgtgctg gccgacaacc
      781 cggccctcac cgtgcggctc gagcagccgt ggccgcgcga gccgtaccgc gtggtggtgg
      841 acggccgcgc gcggctgccc ctcgaggccg ccatgctgcg ggccggcacc cgcgcgcgga
      901 tcctggtggc ggtgggagag gacgcgccgg ccgcgcgggt cgccggtctg gcgtcgagcg
      961 gcgtgaccgt gctgcagtgc aagtcccgcg acgggcgggt ggacgtggtc gacctcagcg
     1021 cgcggctctt cgccctcgac gtcatcgcgg tgctgctcga ggccggcgcc gagctgaccg
     1081 gcgcgttcgt gcaggccggc ctggtcgacc gggtggccgc gttcgtcgct cccaaggtcc
     1141 tgggcggatc cgccgcgccg accccggcgg gcggccccgg ccttcggctg ggcgatgccc
     1201 tccgcctgag cgacctcacc gcgcggccgc tgggcgagga ctggctgctg gaaggccggg
     1261 tggagcgctg acatgttcac cggcctggtc gaggaagtgg ggcgggtgct cgagcgcgcg
     1321 ggctcccgcc tcgtgctcgg cgccgaccgc gtcctcgagg acagcggcgt gggggcgagc
     1381 gtggccgtga acggcgcctg cctgaccgtg gtcgaggtgg gcgcgggcag gctgcgcttc
     1441 gacgtcgggc ccgagacgct ggcgcgcacc gcgctcgggg acctggcggt cggcgatccg
     1501 gtgaacctgg agcgcccgat gcggctcggg ggcctggtcg gcggacacct gatccagggg
     1561 cacgtcgatg gggtgggcac ggtggccgcg ctcacccggc aggcagagac cgcccggctc
     1621 acgatagaat ggccgggcga ggcgctggcc ccgctgctga tcccgcaggg ctcggtggcg
     1681 gtggacgggg tcagcctcac ggtggcgcgg ctcaacgagc gggatttcga gatcatggtc
     1741 atcccgcaca cgctggccgc gaccaccctg gggggactga cgcggggcag gcgggtgaac
     1801 ctcgagatgg atatgatcgg caagtacgtg cagcgcgtct tgcagctgcg ggagccgccg
     1861 gccccccgga aggggccgga cgcgagagag gggacccggt gagcgagtcg gagttcgttc
     1921 cgatcgagga aggcatcgag gagatccgcg ccggccgcat gctcatcgtg gtcgacgacg
     1981 aggaccgcga gaacgagggt gacctgctca tggccgccga gaaggcgacc ccggaggcgg
     2041 tcaacttcat ggccaagtac gggcgcgggc tcatctgcat gcccatgctc ggcgagcgcc
     2101 tcgacgagct gcagatctcg atgatggtct ccgacaacac cgcgccgctc ggcaccgcgt
     2161 tcacggtcag cgtggacgcc cgccggggcg tgaccaccgg aacctccgcc tacgatcgcg
     2221 cggtcaccat ccgcacgctg gtggatgcga cgacgcggcc ggaggacctc acgcgccccg
     2281 gccacatctt cccgctgcgg gccatgccgg gcggcgtgct ccgccgcgcc ggccacaccg
     2341 aggccgcgat cgacctggcc cgcctggccg gctgcgcgcc cgccggggtg atctgcgagg
     2401 tgctcgacga cgaaggcggc atggcgcggc ttcccgagct gatggcgctc agtcgccagt
     2461 accggctcaa ggtgatcacg atcaaggccc tgatcgagta ccgcatccac aaggagaagc
     2521 tggtccgccg cgccgccacc acccgcatgc ccaccgagta cggcgagttc caggtcatcg
     2581 tctacgagac cacggtggac gagcggcagc cgctggccat ggtcctgggc gacgtcaaga
     2641 cggacgaccc ggtgctggtg cgcgtgcact cggagtgcct gaccggcgac gtgttcggct
     2701 cgctccgctg cgactgcggg ccccagctcc acaaggcgct cgccatcatc cagcacgccg
     2761 ggcgcggcat cctggtctac atgcgccagg agggccgcgg catcgggctg ctgaacaagc
     2821 tcaaggccta cgaacttcaa gatgcgggca aggacaccgt cgaggccaac cacgccctcg
     2881 gcttccgggc cgacctccgc cactacggaa tcggcgccca gatcctggtc gacctcgggg
     2941 tcaagaacct caagatcttg accaacaacc cgaagaagat cgtcgggatc gagggctacg
     3001 gcctcaaggt ggtcgagcgc ctgccgctcg aggtgccggc caccgacgcg aaccggagct
     3061 acctgcgcac caagcgcgag aagctcgggc atctcttctc gtccttcccg gactagccca
     3121 tggccggcat cgctccgaag cgctccgtcg cccccgccgc ccgcgggcag cgcttcgcag
     3181 tggtggtggc ccgcttcaac gagcccatct ccaagaggct gctcgacggt gccctcggcg
     3241 cgctcgccga ccacgcggtg accggcgacc tggtggaggt gcactgggtg cccggctcct
     3301 tcgagctcgg ccaggccgcg atgacgctgg cccgcggcgg gcggttcgcg ggcatcgtgt
     3361 gcgtgggggt ggtgatcaag ggagcgaccc cgcacttcga ctacgtctgc agcgcggcgg
     3421 cccacgggat tgcccgcgcc gggctcgaca ccggggtgcc ggtcaccttc ggggtgatca
     3481 ccgcgctgac cgaggagcag gcgtgggagc gcgcgggcgg ggcggtcggc aatcgcggcg
     3541 aggaggccgc gctggccgcg ctcg
//
LOCUS       DATMCV010000934         2659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_585298, whole genome shotgun sequence.
ACCESSION   DATMCV010000934 DATMCV010000000
VERSION     DATMCV010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585298"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VNU03_21385"
     CDS             <1..261
                     /locus_tag="VNU03_21385"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HWP77089.1"
                     /translation="EVIVCLGVTAARSVLQRRVTISASRGEPLTSPEGYRTLVTIHPS
                     AILRVPDERNREAEEHRFVDDLRRAARIAHHAVRGREEETTR"
     gene            443..1432
                     /locus_tag="VNU03_21390"
     CDS             443..1432
                     /locus_tag="VNU03_21390"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWP77090.1"
                     /translation="MIQPTLLGDELGTETTIRMPGQQNCWAIVLAGGEGLRLRSLVRQ
                     VLGDDRPKQYVRLLGARTLLRQTLDRIGLAIPETRTLVVTVRHHAGYVAEEFATAPRP
                     TILVQPVDRGTAAGVLYPAHWIAWRNPEAIVAIFPSDHFLLGEASFMAHVAEVAAWVE
                     KNPDRLVLLGAQPTSPEVEYGWIEPGEGLGELSSGPVRAVRQFWEKPAVARARACMTA
                     GHLWNTSIIVGKVATLVQTGCQALPELSDRLARIEPFVGTPEEPDAVRQAFELMPKVN
                     FSRAVLEACPEALSVSRLPRIVWSDLGSPHRVMEALTRMRIRPEWARSLAPTA"
     gene            complement(1472..1744)
                     /locus_tag="VNU03_21395"
     CDS             complement(1472..1744)
                     /locus_tag="VNU03_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77091.1"
                     /translation="MIERNELEGSLLAEINRRTKEISETQHELARQQRILSKAATLLR
                     LGKGAPTVLAEIREQCPDLLREYCDLQMTLTPPPPLRPVPRAAASA"
     gene            2075..>2659
                     /locus_tag="VNU03_21400"
     CDS             2075..>2659
                     /locus_tag="VNU03_21400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77092.1"
                     /translation="MSSKPTILIVDDEEDSRAALQAILGTWGYATDVASDGREALQKA
                     AGLRPSLVVTDLMMPDMDGLSLLTALQQEMPRMPVIILTGRATVDTAVGAMRQGAYDY
                     LTKPVDLDRLRLLIEKALERGRTLDEITILRRRVKEVWGLGRLIGRSAPMQEVHRLIE
                     QAAATPAPVLIHGETGTGKELVARTLHELSARASG"
BASE COUNT          386 a          953 c          919 g          401 t
ORIGIN      
        1 gaggtcatcg tctgcctggg cgtgaccgcg gcccggtcgg tcctccagcg ccgggtcacc
       61 atcagcgcga gccggggcga gcccctcaca tcgccggagg gctaccggac attggtgacc
      121 attcatccct ccgcgatcct ccgcgtcccg gatgagcgga atcgcgaggc cgaggagcat
      181 cgcttcgtcg acgacctccg gcgcgcggcc cgcatcgccc accacgccgt ccgggggcga
      241 gaggaagaaa cgacccggta gatcttcctg agtgcgcgcg gtcgcgcggc gacccgattg
      301 gacccgatcc ggaggacccg cacaaatcgc gggcggcgga agcgggccga tcgtcacccc
      361 ggtggcacgt cgcttgaatt ccctccgcgc gccaagacgc gccgaccatt cgacctgcgg
      421 cctgcgaggc cgggaggaga ccatgattca gccgacgttg ctgggggacg agctcgggac
      481 cgagaccacg atccgcatgc ccgggcagca gaactgctgg gccatcgtcc tcgcgggcgg
      541 cgaaggcctg cgcctgcgct cgctggtacg ccaggtgctg ggcgatgacc ggcccaagca
      601 atacgtccgc ctgctgggcg cccggaccct gctgcgccag accctggacc gcatcgggct
      661 ggcgatcccg gagactcgca ccctggtcgt gaccgtccgg catcatgccg gctacgtcgc
      721 cgaggaattc gccactgccc cccgcccgac catcctggtc cagccggtcg accgcggcac
      781 cgccgcggga gtgctgtacc cggctcactg gatcgcgtgg cggaacccgg aggcgatcgt
      841 cgccatcttc ccctccgacc acttcctcct gggtgaggcg agcttcatgg cccacgtggc
      901 cgaggtcgca gcgtgggtcg agaagaaccc cgatcgcctc gtgctgctgg gcgcgcagcc
      961 gacgtcgccc gaggtcgagt atggctggat cgagcccggg gagggcctgg gcgagctatc
     1021 cagcgggcca gtccgtgcgg tccggcagtt ctgggagaag cccgcggtcg cgcgggcgcg
     1081 cgcgtgcatg accgccgggc acctctggaa cacctcgatc atcgtcggca aggtcgcgac
     1141 gctggtccag acgggctgcc aggcgctgcc cgagctgagc gatcgcctgg cccggatcga
     1201 gcccttcgtg ggaacgccgg aggagcccga cgcggttcgc caggccttcg agctgatgcc
     1261 gaaggtgaac ttctcgcgcg cagtcctcga ggcgtgcccg gaggcgctga gcgtctcacg
     1321 gctcccccgc atcgtctggt cggacctggg aagccctcac cgcgtgatgg aggccctcac
     1381 ccggatgcgg atccgtccgg agtgggcccg ctcactggcc ccaacggcct gagcccgcga
     1441 cggcggaccg ggacgacgcg gcggccggcc gtcaggccga ggccgccgcg cgcgggacgg
     1501 gacgcagcgg cgggggcggc gtcagcgtca tctgaaggtc gcaatattcc cgcagcagat
     1561 ccgggcattg ttcccggatc tccgccagca cggtgggtgc ccccttgccg agccgcagga
     1621 gcgtggccgc cttcgaaagg atgcgctgct gccgggccag ctcgtgctgt gtctccgaga
     1681 tctccttggt gcgtcggttg atctcggcca gcagcgaccc ttccagctcg ttccgttcga
     1741 tcatcgcccg ctcctttccg cgtcctgatg cccggtgagc cgccaagtga gcctctcggg
     1801 taaagcaagc gggttgccac gccggtcgtc cgcttcggag cctatttgtc gggtgggtcg
     1861 tgacgaggcc cggggttcgg tgcccctacc gcgcgatcct taccgggtag gaatttctcg
     1921 cccgcctcgc tccaccacca ccctgggtcg ttattaccgc gcccgtatgt agcatcgcgc
     1981 agcgcgagcg cgcttcgcac gactggttcg cgtgtgttgt cgcagccttg cactcgggga
     2041 gcaacgggca tggtgattgc tcgataggtg ttccatgtca tcgaaaccga ccatcctgat
     2101 cgtcgacgac gaggaggact cgagggccgc gctccaggcg attctcggga cctggggcta
     2161 cgcgaccgac gtggcctccg acgggcgcga ggcgctgcag aaggcggccg gcctgcgccc
     2221 atcgctcgtg gtgaccgatc tcatgatgcc ggacatggac ggcctcagcc tgctgaccgc
     2281 gctgcagcag gagatgcccc gcatgccggt catcatcctg accggccggg ccaccgtcga
     2341 caccgcggtg ggcgcgatgc ggcagggcgc ttacgactac ctgaccaagc cggtggatct
     2401 cgaccggctc cggctgctca tcgagaaggc gctcgagcgc ggccggacgc tcgacgagat
     2461 cacgatcctg cgacggcgcg tgaaggaggt gtggggcctc ggccgcctca tcggacggag
     2521 cgcgcccatg caggaggtcc accggctcat cgagcaggcc gcggccaccc ccgcccccgt
     2581 cctcatccac ggcgagaccg gcaccggcaa ggagctggtc gcccgcaccc tccacgagct
     2641 ctccgcccgc gccagcggc
//
LOCUS       DATMCV010000935         2342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_921675, whole genome shotgun sequence.
ACCESSION   DATMCV010000935 DATMCV010000000
VERSION     DATMCV010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921675"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..903
                     /locus_tag="VNU03_21405"
     CDS             190..903
                     /locus_tag="VNU03_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77093.1"
                     /translation="MNAVDFEVHAGELRSVIGPNGAGKSTFFKLIAGELPPSSGRVVF
                     DGRDVTGLPQFARSQRGIAKSYQVTTIFPHLTVWENVRVAAQCRQSVMNCWTRADRLP
                     SLADETREILRAVELDGKADLLATHLGHGLQRHLEVAIALACRPRLLLLDEPTAGMSP
                     EETERTIALIRRIATGRTVIVVEHKMKVVMTISDRITVLHQGAVLAEGTPEQIRTDDR
                     VQEVYLGRRARARSAAAPV"
     gene            915..1616
                     /locus_tag="VNU03_21410"
     CDS             915..1616
                     /locus_tag="VNU03_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77094.1"
                     /translation="MLRLEEVHSYYGASHVVQGVSLSVARGETVALVGRNGAGKTTLL
                     KTIMGIVPARRGRVRLDETDVTSLPTHEIARRGITLVPEDRRIFPRLTVAENLEVAAL
                     GRGGGDGTALDRVLEYFPRLRERLDQAGQNLSGGEQQMLALARGFLTRPDLMLIDELS
                     GGLMPMLVDALMEILQRINRDGVTILLVEQELEVALALASRCYVLDQGRIQFEGPAEA
                     LRRDHEIQARFLGAT"
     gene            1651..>2342
                     /locus_tag="VNU03_21415"
     CDS             1651..>2342
                     /locus_tag="VNU03_21415"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77095.1"
                     /translation="MNRRRFITTTAAAGLGAGLFPGAPRAADPIKIGVLLPFSKAFAV
                     LGENTFAGFMLGAKEAGPTIAGRPYTIVKEDDTADPSVGLSKVRKLIDKDEVDVIVGT
                     IHSGVAAAIRNPIVDAKKLWLNPIATNDLLAEQGCSRYHFRFSNSAWQSAAPLGPWAK
                     AKLGDRAYICVFNFAFGQQSAAHFKKAFTEAGGTVVGESFPPFNNTNYAPYFPAIKDA
                     KPQFVFANFAGA"
BASE COUNT          352 a          851 c          808 g          331 t
ORIGIN      
        1 ggccgtaagt cgtcgcgccg ggggagcggc tcctcgccct tccccggcgc gccggccccg
       61 gtcccgcatt gcgttggcgg ggccgatcac ttagggtact aggcgttctc gacgaggagc
      121 gacgacctgg tcccgactac catcctgcgc accgagaagc tcacgcgaag cttcggccgg
      181 ctcctggccg tgaacgcggt ggatttcgag gtgcacgccg gcgagctgcg gtcggtgatc
      241 ggacccaacg gggcgggcaa gagcaccttc ttcaagctca tcgcgggcga gctgccgccc
      301 agctcggggc gcgtggtctt cgacggccgg gacgtgaccg gcctgcccca gttcgcccgc
      361 tcgcagcgcg gcatcgccaa gtcctaccag gtcaccacga tcttcccgca cctcacggtc
      421 tgggagaacg tccgggtcgc ggcccagtgc cgccagagcg tgatgaactg ctggacgcgc
      481 gccgaccggc tgccgtcgct ggccgacgag acccgcgaga tcctgcgcgc ggtcgagctc
      541 gacggcaagg cggacctcct cgccacccat ctgggccacg gcctgcagcg gcacctcgag
      601 gtcgccatcg cgctggcctg ccggccccgc ctcctcctgc tcgacgagcc caccgcgggc
      661 atgagccccg aggagaccga gcggaccatc gcgctcatcc ggcgcatcgc gaccggccgg
      721 accgtgatcg tggtcgagca caagatgaag gtcgtgatga cgatctcgga tcgcatcacg
      781 gtgctgcacc agggcgcggt gctcgccgag ggcaccccgg agcagatccg caccgacgat
      841 cgcgtccagg aggtgtacct gggccgccgc gcgcgggcgc gatccgccgc ggcgccggtc
      901 tgagccccgc cgcgatgctg cgcctcgagg aggtgcacag ctactacggg gcgagccacg
      961 tcgtgcaggg cgtgagtctc tccgtcgcgc ggggcgagac ggtggcgctg gtgggacgga
     1021 acggggcggg gaagaccacc ctgctcaaga ccatcatggg catcgtgccc gcgcggcgcg
     1081 gccgggtgcg gctcgacgag accgacgtga cgagcctccc cacccacgag atcgcccggc
     1141 gcggcatcac cctcgtcccc gaggaccggc gcatctttcc gcggctcacg gtggccgaga
     1201 acctcgaggt cgccgccctc gggcgcggcg gcggggacgg gaccgcgctc gaccgggtgc
     1261 tcgagtactt cccgcgcctc cgcgagcgcc tcgaccaggc ggggcagaat ctctccggcg
     1321 gcgagcagca gatgctcgcg ctcgcccgcg gcttcctgac ccgcccggac ctgatgctga
     1381 tcgacgagct gtccggcggg ctcatgccga tgctggtcga cgcgctgatg gagattctcc
     1441 agcggatcaa ccgggacggc gtcaccatcc tgctcgtgga gcaggagctc gaggtggcgc
     1501 tggcgctcgc gtcccgctgc tacgtgctcg accagggccg gatccagttc gagggcccgg
     1561 ccgaggccct gcgccgcgat cacgagatcc aggcgcggtt cctcggcgcc acctgacgtc
     1621 cagaccctac tttctcgcga ggaggattcg atgaaccgac ggcgattcat cacgaccacg
     1681 gcggcggcgg ggctcggggc cgggctgttc ccgggcgcgc cgcgcgccgc cgatcccatc
     1741 aagatcgggg tgctgctgcc gttctcgaag gcgttcgcgg tgctcggcga gaacaccttc
     1801 gccggattca tgctcggggc caaggaggcg gggccgacga tcgcggggcg gccgtacacg
     1861 atcgtcaagg aggacgacac cgccgatccg agcgtcgggc tcagcaaggt gcgcaagctc
     1921 atcgacaagg acgaggtcga cgtgatcgtg ggcaccatcc acagcggggt cgcggcggcc
     1981 atccgcaacc ccatcgtgga cgccaagaag ctctggctca acccgatcgc caccaacgac
     2041 ctgctcgccg agcagggctg ctcccgctac cacttccgct tctccaacag cgcctggcag
     2101 tcggccgccc cgctcggccc ctgggccaag gccaagctcg gggatcgcgc ctacatctgc
     2161 gtgttcaact tcgccttcgg ccagcagtcg gccgcccact tcaagaaggc gttcaccgag
     2221 gcgggcggca cggtggtcgg cgagtccttc ccgccgttca acaacacgaa ctacgcgccc
     2281 tatttcccgg cgatcaagga cgccaagccg cagttcgtgt tcgccaactt cgcgggggcc
     2341 ga
//
LOCUS       DATMCV010000936         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_48686, whole genome shotgun sequence.
ACCESSION   DATMCV010000936 DATMCV010000000
VERSION     DATMCV010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48686"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VNU03_21420"
     CDS             <1..984
                     /locus_tag="VNU03_21420"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP77096.1"
                     /translation="LAESMRAYNRCGTTSIFEGHGISAEVLAAYQALRERGGRPGVRA
                     HLLWSPAWPSAEPAAVGELLRTWGAWLARRGLGDEFLRMGGLYTESDYSEENRLRGTT
                     SPYTGWAGVSYKAALPEDVMVEMMMEAARSHIRIGSFTPNILDLYERVNRAVPIADRR
                     WIIEHIGVYGPEEIARIRDLGLVLTSYTGRYIWQDGATLARQLGPAGASHIAPLRALR
                     EAGVHVALSTDNVPPSLFDSIHHAVARRPRGATAAVGPDQRLTREEALACASREGAYL
                     TFEEDVKGTLEVGKLADVAVLDQDVLTVPEDAIPGIRADLVVVGGAVVLET"
     gene            complement(981..1133)
                     /locus_tag="VNU03_21425"
     CDS             complement(981..1133)
                     /locus_tag="VNU03_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77097.1"
                     /translation="MIRHLPSGEYRLYSRKKNPRTGRRRNLGTFKTRAAAERHERAVQ
                     FFKRGG"
     gene            complement(1538..1861)
                     /locus_tag="VNU03_21430"
     CDS             complement(1538..1861)
                     /locus_tag="VNU03_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HWP77098.1"
                     /translation="MDTVTATIEDLRRRGFTDHFMVRGSQLLAIDAGRPFLPRDVVIR
                     EYHRFEGVSDPDDMAIVYALESREGTKGVLTDAFGAYSDPAVGDMLDILRIEPGKTAA
                     RLATP"
     gene            complement(1917..>2126)
                     /locus_tag="VNU03_21435"
     CDS             complement(1917..>2126)
                     /locus_tag="VNU03_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019765783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77099.1"
                     /translation="CRAFGLGTTITTNHIRCEDEVKAVLGVPDDVQTFALMPIGYPLD
                     SFGPVVRRPVAEVVYADRWARRWPG"
BASE COUNT          334 a          749 c          733 g          310 t
ORIGIN      
        1 ctcgccgagt ccatgcgcgc ctacaaccgg tgcggcacca cgagcatctt cgagggccac
       61 ggcatctcgg ccgaggtgct cgcggcctac caggcgctcc gcgagcgcgg cggtcgaccc
      121 ggcgtgcggg cccacctgct gtggagcccg gcctggccgt cggccgagcc cgccgcggtc
      181 ggcgagctgc tacggacctg gggcgcatgg ctcgcccggc gcggcctcgg cgacgaattc
      241 ctgcgaatgg gcgggctcta caccgagtcg gactactcgg aggagaaccg gctgcggggg
      301 acgacgtccc cctacaccgg ctgggccggc gtctcgtaca aggcggcgct gcccgaggac
      361 gtgatggtcg agatgatgat ggaggccgcg cgcagccaca tccgcatcgg cagcttcacc
      421 ccgaacatcc tcgatctcta cgagcgggtg aaccgcgcgg tgccgatcgc ggaccgccga
      481 tggatcatcg agcacatcgg cgtctacggg ccggaggaga tcgcgcgcat ccgggacctc
      541 ggcctggtgc tgacgagcta caccgggcgc tacatctggc aggacggcgc cacgctcgcc
      601 cgccagctcg gccccgcggg cgcctcccac atcgcgccgc tccgcgcgct ccgggaggcg
      661 ggcgtccacg tggcgctctc gaccgacaac gtgccgccgt cgctcttcga ctcgatccac
      721 cacgcggtcg cccgccggcc gcgcggcgcc accgcggcgg tcggcccgga ccagcgcctc
      781 acgcgcgagg aagcgctcgc ctgcgcctcg cgcgagggcg cctatctcac gttcgaggag
      841 gacgtgaagg gcacgctcga ggtcggcaag ctggccgacg tcgcggtcct cgaccaggac
      901 gtcctgaccg tccccgagga cgcgatcccc ggcattcgcg cggacctcgt cgtggtgggc
      961 ggcgcggtgg tgctggagac ctagccgccc cgcttgaaga actgcaccgc ccgctcgtgg
     1021 cgctcggcgg ccgcgcgggt cttgaacgtc cccaggttac ggcgacgtcc ggtgcggggg
     1081 ttcttcttcc gcgagtacag ccggtactct cccgacggca gatgacggat catgtcggcg
     1141 gaaaccagca agctcggtgc caggggacgg cctcattgca ccgcgagatg ctccaataga
     1201 tatgcgccga gcccgagagc gcccacgatc agcaccgcgg ttgtcaggac tcggtagcga
     1261 tcccagaatg ccagcaaacg atagagcatg tcctcctccg tctcgcgtca atgcccttca
     1321 cctctgagac gatccaggcg gcagcggcat gcagcccggt gccccggcac ccccagggaa
     1381 ccttgtaccg ggcaggaact tcccgagctg cgaagcacgg cgtcagcaag ttcccaatat
     1441 tccctcaatc cctcggtgcc gtccgatggc atccgcgatg cttagcccgg caggaggagg
     1501 ccaagaagca atccctcgtc cgggatggga accggactta cggggtggcc aagcgggcgg
     1561 cggtctttcc cggctcgatg cgcaggatat cgagcatgtc cccgacggcc ggatcggaat
     1621 acgcgccgaa ggcgtcggtc agcacgccct tcgtgccttc ccggctctcc agggcataga
     1681 cgatcgccat gtcgtctggg tccgagaccc cctcgaagcg gtggtactcc cggatgacga
     1741 cgtcgcgcgg gaggaaaggc cggcctgcgt cgatcgccag cagctggctg ccccggacca
     1801 tgaagtggtc ggtgaagcct cgccggcgga gatcctcgat cgtggcggtg acggtgtcca
     1861 tggcgctccc cgctccgtta gatgctagtc ggggcggatg agagaccggt gaattgtcac
     1921 ccgggccacc ggcgcgccca tcggtcggca tagacgacct cggcgaccgg gcggcggacg
     1981 actggaccga acgagtcgag cgggtagccg atcggcatca gcgcgaaggt ctgcacgtcg
     2041 tccggcaccc cgagcaccgc cttcacctcg tcctcgcagc ggatgtgatt ggtggtgatc
     2101 gtggtgccga ggccgaaggc gcggca
//
LOCUS       DATMCV010000937         5785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_921747, whole genome shotgun sequence.
ACCESSION   DATMCV010000937 DATMCV010000000
VERSION     DATMCV010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921747"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VNU03_21440"
     CDS             complement(<1..1083)
                     /locus_tag="VNU03_21440"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWP77100.1"
                     /translation="MTTTRTEKDTMGTIEVPADRYWGAQTQRSLEHFRIGDDRFPREI
                     IRALGVLKKACALVNRELGLLPEDKARAIVAAADEVIEGRLDAHFPLAVWQTGSGTQT
                     NMNANEVIANRAIEILGGRLGSQQPVHPNDDVNRSQSSNDTFPTAMHIAAVEQIAGRL
                     VPAVSRLRDTLEAKSRAFADIVKIGRTHLQDAVPLTLGQEISGWTSQLDRALRQIRAA
                     MPGILELALGGTAVGTGLNAPRGFGERAALVIADLTGRPFVSAPNKFEALAAHDALVG
                     AHGALRTLAAALTKIANDVRWLASGPRCGIGELRIPENEPGSSIMPGKVNPTQCEALT
                     MVCAQVMGNDVAVGFGGASGHFELNVY"
     gene            1154..2152
                     /locus_tag="VNU03_21445"
     CDS             1154..2152
                     /locus_tag="VNU03_21445"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP77101.1"
                     /translation="MDCCAHEGPAAGDARFDLQRVADGVHVAVAAPAYKVNSNTAIIE
                     SDDGVVIVDTHSKPSAARVIVDRVRELTARPVRFVVNTHFHWDHWHGNEVYPAAYPGA
                     EIITNQLTREAMVNKGLKRIQDHVRQVPAEVARLRGELAAATTTEQRAKLQADLRLAE
                     SYLAEVRALKPALPTMAFERTMKLYRRDREIHLLYLGRAHTEGDVFVYLPKERTVITG
                     DAVIGWTPYMGDGYPEDWVTTIDRLAELDFDTIVMGHGNPAGRDWLRTFRAYIHDMVE
                     AVREEVATGASLDEVKQRVPARLAPTYEKAFATYEHYRPWRAGLLANIERTYAMVS"
     gene            2157..3356
                     /locus_tag="VNU03_21450"
     CDS             2157..3356
                     /locus_tag="VNU03_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWP77102.1"
                     /translation="MAETDLFNPLSPAFHADPYPFYRRLRETDPVHLSALGLWILTRY
                     EDCVTSLRDPRFGRDGFEAILSAQYGEESETGRLPRSMLFRDPPDHTRLRALVNRAFT
                     PRVIEGMRGQIQGVVDLLLDRVERQGRMDVIGDLAYPLPVTVICDMLGVPAGDHEQIK
                     EWSSDIIRSLDAIGIPSDESIVERGRIGRRGIADYFRALLPERRRHPRADLLSSLIAV
                     EEQGDRLTEGELLATCVLLFIAGHETTVNLIGNGLLALLQHPTEVERLRREPALIGGA
                     VEELLRYDSPVQRTARITNADVELGGKTIPKGAFVVTAIGAANRDPAHFPDPDRLDVA
                     RADNRHIAFGFGIHFCLGAPLARVEGQLALGTLLRRMPRLRLGEEALAWRESSTLRGL
                     KALSVNF"
     gene            complement(3405..3905)
                     /locus_tag="VNU03_21455"
     CDS             complement(3405..3905)
                     /locus_tag="VNU03_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWP77103.1"
                     /translation="MTRKRLLSMAIGAALTLTAGAAAAQTPPPPPPAYGAPITLEQAK
                     KVMAGAEAEAKKNNWGMVITILDSGGNVVMLQRMDGTQLGSVDVARDKAWSAVAFRRP
                     TKVFEDAIAQGGANLRLLRLTGASPIEGGIPLMADGKIIGAIGVSGGSAPQDGQVAKA
                     GVDGLK"
     gene            complement(4005..5366)
                     /gene="mgtE"
                     /locus_tag="VNU03_21460"
     CDS             complement(4005..5366)
                     /gene="mgtE"
                     /locus_tag="VNU03_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWP77104.1"
                     /translation="MERQAEQHEAGQIARTLETAHDADVAEALNAIDHAAAARVLAAL
                     PFDLAVRAVNQPELDHRAHLFEHLPTDKAVALMTAMSADQRVEVFRGLSDRARARLMP
                     TIDEHTRASLRALLSYAPTTSGGIMTTEHVEAPATWTVGRALAHIRDSGHPHRPVYAV
                     YVLDPVDRRLVHVVTLRELVMADPARTLLEVGERNPPVTVGPWTHREETARLIAKYDL
                     LAVPVVDEGGHLLGIVTVDDMIDAMVKEQTEDLHKLGGVEALDEPYTKIGFGTMIRKR
                     AGWLCALFLSEMLTATAMQYFEGELSRAIVLTLFIPLVMSSGGNSGSQATSLIIRALA
                     LREVTVGDWWRIAVREIPAGVALGAILGAIGIVRISAWQVFGFYDYGPHWPLIAATVG
                     GALVGVVMFGSLAGSMLPFVLRRVGFDPASASAPFVATLVDVTGLVIYFSVAYLVLRG
                     TLL"
     gene            complement(5380..>5785)
                     /locus_tag="VNU03_21465"
     CDS             complement(5380..>5785)
                     /locus_tag="VNU03_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018863756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP77105.1"
                     /translation="WSSLQRARRRAPGVPLVAGSAEALPFRDACFDTVLSGLVFCSVP
                     DARRGLLEARRVLAPRGRLRMLEHVRSTEPWKARVQDWLQPAWTRVSGGCHPNRDTER
                     AVEASGFDIEIDGRRAHGNLRRFAAAPREPSP"
BASE COUNT          829 a         2117 c         2014 g          825 t
ORIGIN      
        1 gtagacgttc agctcgaagt ggccggacgc gccgccgaag ccgaccgcga cgtcgttgcc
       61 catcacctgg gcgcacacca tcgtgagcgc ctcgcactgg gtcgggttga ccttgccggg
      121 catgatcgag ctgcccggct cattctcggg gatccgcagc tcgccgatcc cgcagcgggg
      181 ccccgaggcg agccagcgca catcgttggc gatcttggtg agggccgcgg ccagggtccg
      241 gagcgcgccg tgcgcaccca cgagcgcgtc gtgggcggcc agcgcctcga acttgttggg
      301 cgcggatacg aacgggcgcc cggtcaggtc cgcgatcacg agtgcggcgc gctccccgaa
      361 cccgcgcggg gcgttgagcc cggtgcccac cgcggtgccg ccgagggcca gctcgagaat
      421 tcccggcatc gcggcccgga tctggcgcag ggcgcggtcc agctggctcg tccagccgga
      481 gatctcctgc ccgagggtca gaggcaccgc gtcctggagg tgggtgcggc cgatcttgac
      541 gatgtcggcg aaggcccgcg acttggcctc gagcgtgtcg cgcaggcgcg acaccgcggg
      601 cacgagccgc cccgcgatct gctccaccgc cgcgatgtgc atcgcggtcg gaaaggtgtc
      661 gttggaggac tggctccggt tgacgtcgtc gttgggatgc accggctgct gcgagccgag
      721 ccgcccgccc aggatctcga tggcccggtt ggcgatcacc tcgttggcgt tcatgttggt
      781 ctgggtgccg ctgccggtct gccagaccgc gagggggaag tgcgcgtcca gacgcccctc
      841 gatcacctcg tcggcggcgg ccacgatggc ccgcgccttg tcctccggca acaggcccag
      901 ctcgcggttc acgagcgcgc aggccttctt gagcacgccg agggcccgga tgatctcgcg
      961 cgggaagcgg tcgtcgccga tgcggaagtg ctcgagcgac cgctgggtct gggcgcccca
     1021 gtagcggtcg gcgggcacct cgatggttcc catcgtgtcc ttctcggtcc gggtcgtggt
     1081 catggatccc tcctcgggtt gcttgagcgt gtccacgcca tcgcatagta tcgggcgcag
     1141 ggagagcaca caaatggact gctgcgctca cgaaggaccg gccgcgggcg acgcccgctt
     1201 cgacctccag cgggtggccg acggcgtcca cgtcgcggtg gccgcccccg cctacaaggt
     1261 caactccaac accgcgatca tcgagagcga cgacggcgtc gtgatcgtcg acacccattc
     1321 caagccctcc gccgcgcgcg tgatcgtgga tcgcgtgcgc gagctcaccg ccaggccggt
     1381 ccgcttcgtg gtcaacaccc acttccactg ggaccactgg cacggcaacg aggtctaccc
     1441 ggccgcctac ccgggcgcgg agatcatcac caaccagctc acccgcgagg cgatggtcaa
     1501 caagggtctc aagcgcatcc aggaccacgt gcggcaggtg cccgccgagg tggcccggct
     1561 gcgcggcgag ctggccgcgg ccaccaccac cgagcagcgg gcgaagctcc aggccgatct
     1621 ccggctcgcc gagtcgtacc tggccgaggt gcgggcgctc aagccggccc tgcccacgat
     1681 ggcgttcgag cggacgatga agctctaccg ccgcgaccgc gagatccacc tgctctacct
     1741 gggccgcgcc cacaccgagg gcgacgtctt cgtctacctg ccgaaggagc gcaccgtcat
     1801 caccggcgac gcggtcatcg gctggacgcc ctacatgggc gacggctatc cggaggactg
     1861 ggtcaccacc atcgaccggc tggccgagct ggacttcgac acgatcgtca tgggccacgg
     1921 caacccggcg ggtcgcgact ggctgcggac tttccgggcg tatatccacg acatggtgga
     1981 ggcggtgcgc gaggaggtcg ccaccggcgc cagcctggac gaggtcaagc agcgggtgcc
     2041 cgcgcgcctg gccccgacct acgagaaggc cttcgcgacc tacgagcact accggccgtg
     2101 gcgcgccgga ctcctcgcca atatcgagcg gacctacgcg atggtgagct agccgcatgg
     2161 ccgagaccga cctcttcaac ccgctctcgc ccgccttcca cgccgacccg tacccgttct
     2221 accggcggct gcgcgagacc gatccggtcc acctgagcgc gctcggcctc tggatcctca
     2281 cccgctacga ggactgcgtc acgtccctgc gcgacccgcg cttcgggcgc gacggcttcg
     2341 aggccatcct ctcggcccag tacggcgagg aatcggagac cggccgcctc ccgcgctcca
     2401 tgctcttccg cgacccgccc gaccacaccc gcctgcgggc gctcgtgaac cgggcgttca
     2461 cgccgcgggt catcgagggc atgcgcgggc agatccaggg cgtggtggac ctcctgctcg
     2521 atcgcgtcga gcgccagggg cggatggacg tcatcggcga tctcgcctac ccgctgccgg
     2581 tgaccgtcat ctgcgacatg ctcggagtgc cggcgggcga ccacgagcag atcaaggagt
     2641 ggtcgtcgga catcatccgg agcctggacg cgatcggcat cccgtccgac gagtcgatcg
     2701 tggagcgcgg gcgaatcggc cgccgcggga tcgcggacta cttccgcgcc ctgctccccg
     2761 agcgccgccg ccacccgcgc gcggacctgc tctcctcgct catcgcggtc gaggagcagg
     2821 gcgaccgcct caccgagggc gagctgctcg cgacctgcgt gctgctcttc atcgcgggcc
     2881 acgagaccac cgtcaacctg atcggcaacg gtctcctcgc cctgctccag cacccgacgg
     2941 aggtcgagcg cctccgccgg gagccggccc tcatcggcgg cgcggtggag gagctgctgc
     3001 gctacgacag tccggtgcag cggacggcgc ggatcacgaa tgccgacgtg gagctgggcg
     3061 gcaagacgat cccgaagggc gccttcgtgg tcaccgcgat cggcgcggcc aaccgcgatc
     3121 ccgcgcactt cccggacccg gatcgactgg acgtcgcccg cgccgacaat cgccacatcg
     3181 cgttcggctt cggcattcac ttctgcctgg gcgccccgct cgcgcgggtg gagggccagc
     3241 tggcgctggg gacgctgctg cgacggatgc cgcggctgcg gctcggcgag gaggccctcg
     3301 cctggcgcga gtcctccacc ctccgcggcc tgaaagccct ctcggtcaac ttctgacccc
     3361 taccctctcc cccgagggga gagggtaggg tgagggggaa cgcgctactt cagaccgtcg
     3421 acgcccgcct tcgccacctg accgtcctgc ggcgcgctgc cgccggagac gccgatggcg
     3481 ccgatgatct tgccgtccgc catgagcggg atccctccct cgatcgggct cgccccggtc
     3541 aagcgcagca ggcggaggtt ggcgccgccc tgggcgatcg cgtcctcgaa gaccttggtg
     3601 ggccggcgga acgcgaccgc ggaccaggcc ttgtcccgcg cgacgtcgac gctcccgagc
     3661 tgcgtgccgt ccatgcgctg cagcatgacc acgttaccgc ccgagtcgag gatggtgatc
     3721 accatgcccc agttgttctt cttggcctcg gcctccgcac cggccatcac cttcttggcc
     3781 tgctcgagcg tgatgggagc cccataggcc ggcggcggcg ggggcggcgt ctgggcagcg
     3841 gccgcgccgg ccgtgagcgt gagtgcggcc ccgatggcca tggacagcag tcgtttcctg
     3901 gtcatacgtc cctccggtgg tgagacgggg tttgtggggc gctgggcgct gcttggcaga
     3961 taactgcgat cctccctcga cagttcccga cgcggcccgg cccgctagag cagcgtgccc
     4021 cgcagcacca ggtacgcgac cgagaagtag atgacgaggc cggtgacgtc gaccagggtg
     4081 gccacgaagg gagccgacgc gctcgcgggg tcgaagccca cccggcggag gacgaacggg
     4141 agcatcgagc cggcgaggga gccgaacatc accaccccca ccagggcccc gcccaccgtc
     4201 gccgcgatca gcggccagtg cggcccgtag tcgtagaagc cgaaaacctg ccacgcggag
     4261 atccgcacga tgccgatggc gcccaggatg gcgcccaggg ccacgccggc ggggatctcc
     4321 cgcaccgcga tccgccacca gtcccccacc gtgacctcgc ggagggccag cgcccggatg
     4381 atcagcgagg tggcctgcga gccggagttg ccgcccgagc tcatcacgag agggatgaag
     4441 agggtgagca cgatggcccg gctcagctcg ccctcgaagt actgcatcgc ggtggcggtg
     4501 agcatctcgc tgaggaacag cgcgcagagc cagccggccc gcttccggat catggtgccg
     4561 aagccgatct tggtgtacgg ctcgtcgagc gcctcgacac cgcccagctt gtgcaggtcc
     4621 tcggtctgct ccttcaccat cgcgtcgatc atgtcgtcca ccgtcacgat gccgaggagg
     4681 tgcccaccct cgtcgaccac cggcaccgcc agcaggtcgt acttggcgat cagccgcgcg
     4741 gtctcctccc ggtgggtcca cgggccgacc gtcaccggcg ggttccgctc gcccacctcg
     4801 agcagggtgc gcgcgggatc ggccatcacc agctcgcgga gcgtgaccac gtgcacgagg
     4861 cgccggtcca ccggatcgag gacgtacacc gcgtagaccg gccggtgcgg gtgcccgctg
     4921 tcgcggatgt gggcgagggc gcgcccgacc gtccacgtcg cgggcgcctc cacgtgctcg
     4981 gtggtcatga tgccgcccga ggtggtcggg gcgtagctca ggagcgcgcg gagggaggcg
     5041 cgggtgtgct cgtcgatcgt gggcatcagc cgcgcccggg cgcggtccga gagaccgcgg
     5101 aagacctcca cccgctgatc ggccgacatc gcggtcatca gggccaccgc cttgtcggtg
     5161 ggcaggtgct cgaagaggtg cgcccggtga tcgagctcgg gctggttgac cgcgcgcacc
     5221 gccaggtcga acggcagcgc ggccagcacc cgcgccgccg ccgcgtggtc gatggcgttc
     5281 agcgcctccg ccacgtcggc gtcgtgcgcg gtctccaggg tccgcgcgat ctgcccggcc
     5341 tcgtgctgct ccgcttgacg ctccatgatc gggtctcccc tagggactcg gctctcgcgg
     5401 ggccgccgcg aagcggcgca ggttgccgtg ggcgcgacgc ccatcgatct cgatgtcgaa
     5461 gccgctcgcc tcgaccgctc gctcggtgtc gcggttggga tggcatccgc cgctcacccg
     5521 cgtccaggcc ggctgcagcc agtcctgaac ccgggccttc cacggctcgg tcgagcgcac
     5581 gtgctccagc atccggagcc gcccacgcgg cgcgagcacg cgccgggcct cgagcaagcc
     5641 ccgccgggca tccggcacgc tgcagaagac gaggccgctc agcaccgtgt cgaagcacgc
     5701 gtcgcggaac ggcagggcct ccgcgctccc ggcgaccagc ggcactccgg gcgcacggcg
     5761 ccgggcgcgc tgcagcgacg accag
//
LOCUS       DATMCV010000938         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_456497, whole genome shotgun sequence.
ACCESSION   DATMCV010000938 DATMCV010000000
VERSION     DATMCV010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456497"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VNU03_21470"
     CDS             <1..1155
                     /locus_tag="VNU03_21470"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWP77106.1"
                     /translation="LRRWLELSGYQARLVLNVTDINDKIYDAARAQGISSAELARQAT
                     QWYFEDTSALGLGRPDVEPLATETVPEIVALIGELMARGLAYESAGDVYFCVTRFPDY
                     GRLSGARPEDMAAQEPGPRKEDPRDFALWKANKRHEDTWWPSPWGQGRPGWHIECSAM
                     AEKHLGPRFEIHGGGLDLRFPHHENEIAQSRGAGREFARLWTHNGMLELHAEKMSKSV
                     GNVVSLRGALESWGREPLLVFFLNAHYRSPVEYSDAAMEAARAQVADFRAAARVAEPR
                     PSAFTWDGFRAALDDDFDTPRALSILHAWRAAGQVELLGRGLRVFGLVIDADGTDAPP
                     EAHRLAEARQAARRRRDFRDADRLRSEIDRLGWDVQDVAGGAFRLVRKRR"
     gene            complement(1328..1822)
                     /locus_tag="VNU03_21475"
     CDS             complement(1328..1822)
                     /locus_tag="VNU03_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77107.1"
                     /translation="MDGAAIHALVRAPGHDVPTGLVLPASDLYGGLASWLGLHVPTSA
                     WFIAEGDAVKTGLVPALFDGPGEAASTFGFFEPDGVALLIRAAAPPGLADGTIYLGVR
                     AYGNLRVGERLRAATQAWDKAGRPRVDRLRIRAYPIESPYAPRSGDIVRERPCTRLVL
                     AWPP"
BASE COUNT          314 a          685 c          733 g          295 t
ORIGIN      
        1 ctccggcggt ggctcgagct atcaggctac caggcgcggc tggtcctgaa cgtcaccgac
       61 atcaacgaca agatctacga cgcagcgcgg gcccaaggaa tcagcagcgc cgagctggcc
      121 cgacaggcga cccagtggta cttcgaggac acgagcgccc tcggtctcgg ccggccggac
      181 gtcgagcccc tggccacaga gactgtcccc gagatcgtcg cgctcattgg ggagctgatg
      241 gctcgggggc tagcgtacga gtcggccggc gacgtgtact tctgcgtgac gcggttcccg
      301 gactacggcc ggctctccgg cgcgcggccg gaagacatgg ccgctcagga gcccggcccg
      361 cggaaggaag acccgcggga ctttgcgctg tggaaagcga acaagcggca cgaggacacg
      421 tggtggccgt ctccctgggg ccagggccgt cccgggtggc acatcgagtg ctccgccatg
      481 gccgagaaac acttgggtcc gcgctttgag attcacggcg gcgggctcga cctccgcttc
      541 ccgcatcatg agaacgagat cgcacaatct cggggcgccg ggcgggaatt cgcgcggctg
      601 tggacccata acggcatgct cgagctgcac gccgagaaga tgtcgaagtc ggtcggcaac
      661 gttgtctccc tccgtggggc gctggagtcg tgggggcgtg aaccgctctt ggtgttcttc
      721 ctgaacgcgc actaccggag cccggtcgag tattccgacg ccgcaatgga ggcggcgcgt
      781 gcccaagtcg ccgatttccg tgctgcggcc cgcgtcgcgg agccgcgccc cagcgcgttc
      841 acctgggacg gctttcgggc cgcgctcgac gacgacttcg atacaccgcg cgcgctctcg
      901 attctccacg cttggcgcgc ggccggccag gtcgagcttc ttgggcgcgg tctccgtgtc
      961 ttcggcctag tcatcgacgc cgacggcacc gatgccccac ccgaggcgca ccggctcgcc
     1021 gaggcacgtc aggccgcgcg gcggcggcga gattttcgtg atgccgaccg cttgcggagc
     1081 gaaatcgacc ggctcggatg ggacgtgcag gacgtcgcgg gtggcgcatt ccgtctcgtc
     1141 cgcaagaggc ggtgaggcgc cgggcgcgca cgctttccga gcctttacct atcggatatg
     1201 gatcaacgtc ctccgtgctc ctcccagacg cagacgcctg gacacaggac ttccaatccc
     1261 gcgtgcggtt ccgagttgag ttgccccgaa gctgctccgg gcgtgacacg accacctcgc
     1321 agaatgctca gggtggccag gcgagaacga gccgcgtgca cggtcgctct cgcacgatat
     1381 cgcccgatcg cggggcgtac ggactttcga tgggataggc gcggatgcgg aggcggtcga
     1441 cgcgcggccg cccagccttg tcccacgctt gcgtggctgc gcgcagccgc tccccgaccc
     1501 tcagattgcc gtaggcacgg acgcccaagt agatggtgcc gtccgcaagg ccgggcgggg
     1561 ctgcggcgcg gatgaggagg gccacgccgt ccggctcgaa gaagccgaac gtgctcgccg
     1621 cctcgccggg accgtcgaag agcgcgggga cgagtcccgt cttcactgcg tcgccctcgg
     1681 cgatgaacca ggcggaggtc gggacgtgga gcccgagcca cgaggcgagg ccgccataca
     1741 ggtcgctcgc cggcaggacg agtccggtcg ggacgtcatg acccggcgcg cggaccaggg
     1801 cgtggatggc ggcgccgtcc accgtgtggc tcccgcgcgg ccatacggcc ggcgcaggct
     1861 cggggccgac gggtacacgt cgccgcggcg acagggacat gcgggatgaa gaggtgccgg
     1921 ggaacggcgg cgaacgcctc cgccagcgcg ggcgagcgca ggcagtcctt cgaccgcagc
     1981 gcctcgacga gcgcaccgcg gagggcatcg gaggcggcgc tccttgt
//
LOCUS       DATMCV010000939         4816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_649430, whole genome shotgun sequence.
ACCESSION   DATMCV010000939 DATMCV010000000
VERSION     DATMCV010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649430"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VNU03_21480"
     CDS             complement(<1..700)
                     /locus_tag="VNU03_21480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP77108.1"
                     /translation="MEYTRISADCHIDMPWMPPDLFTANATAALRDRMPYVTDGPDGP
                     RWTSRNGAAFGLAGGVGPSGQKYVPGVHYRADVMASTGLYDDGRKGIRRPTDPHLRVA
                     DMERDGVQAEVIFGILGAATRLGDHEAAAEMFRIYNDWLLDFCRHYPERHVGLACLPY
                     GDIPAAVREIHRVAKMGLRGLELSCSWDMEPMWHPVWEPLWQAVSEVSLPLHFHTFPT
                     MPPSVREKAVGLTKR"
     gene            782..1831
                     /locus_tag="VNU03_21485"
     CDS             782..1831
                     /locus_tag="VNU03_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWP77109.1"
                     /translation="MTMKTVVALPGEGIGIEVVDATCDLLMGTGLPLKILTPPQGSPL
                     PEETKRLAREADGVLFGAAGPSTSPVVSWLRWEMAAWAGVRPIRFFPGMRSPLTDPDG
                     IDYVLLRENSEGLYPGREGDLADLVRLMPNLSDRTGRTVSQFGTEGRFAVKVVTPRGA
                     ERIARFACDLARKRQARGKPGKVTLVTKSNVLPKTDGLYDEIVGRIVREAGLPFEHFH
                     VDDAARRLVRFPKAMDVVLCMNLYGDILSDLGAETAGGLGLAPSGNFGDNWAYFESVH
                     GSAPDIAGKGIANPTATILSAALMLEHMGLGAEAQRLEAAVGRVYRDGKVLTPDQGGS
                     ARTRDMAQAVLAAYR"
     gene            1845..3314
                     /gene="tcuA"
                     /locus_tag="VNU03_21490"
     CDS             1845..3314
                     /gene="tcuA"
                     /locus_tag="VNU03_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HWP77110.1"
                     /translation="MSDWDVIVVGAGNAALAAAVSAREQGAGRVLALEKAPRALRGGN
                     THYSGGLLRIAFDRPDDLRPLVPDAEREVPGFYAGVEPYPHRLFMADLLRVTDGRTDP
                     ELAKLLIGRSHDTACWMARQGIEMEPAVSLSAVKVGDTYKWSPGAVIRARHEGVGLSA
                     MWFKIAEREGVEVRYDTAAERLLQDRSGRVTGVAARGPNGLEELSARAVVLGCGGFEA
                     NPEWRARYLGRPWDLAKVRGTRYNTGDGLRMAMEVGALPHGQWTGCHSTPIDADAPPH
                     GDRKLTDKTNRLSYPYGVLVNTRGQRFFDEGEDFQFYTYAKLGGIILNEPGGVGWQIF
                     DAKVTHLLEGRYQTGTPVVAHTLEGLVEKLPLDQAACRRALEAYNAAVTPGRFDPTVR
                     DGLATRGLTLPKSNWAQRLDTPPYSAYPVTGGITFTFGGVRVTNRAQVVSMSWSVIAG
                     LYACGEMVGGLFHGNYPGGTGLMSGAVFGRLAGAEAAGA"
     gene            3337..4269
                     /locus_tag="VNU03_21495"
     CDS             3337..4269
                     /locus_tag="VNU03_21495"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP77111.1"
                     /translation="MTSKPRIGFVGLGTMGGPMARRLVASGYEVAGHDPSPDRARQAA
                     AAGVTLEASPAAAAKAAEVVLSSLPDPAAVRQAYLGPDGVLSTARPGTVLVDMSTIDP
                     DTCREVAAAARTGGIDCLDAPVSGGPVEAGNGKLVFLLGGDAAVLERVRPLLLTLGTE
                     LHHIGPLGSGLVVKIVNNMMSMANVAVAAEAMTLGVKAGVDPQRLFDILSTSGGRSHH
                     FLKRFPNVLAGDFTPNFSIGLARKDVSLALAMAARLEMPVPLASIVRQVYEAAHAQGF
                     GQLDMAGVTRLYEQWTGVAVRGAAARDAEKPTAG"
     gene            complement(4282..>4816)
                     /locus_tag="VNU03_21500"
     CDS             complement(4282..>4816)
                     /locus_tag="VNU03_21500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP77112.1"
                     /translation="LIAETEVLPPGRALDVACGAGRNAVWLARRGWRVTGVDFSEVAL
                     RAARDLAAGSGVEIEWVEADAVTWAPPGRAYDLVTVMYLQLPAHERRVALAHAASAVR
                     PGGTLLVVGHDLLNLTEGSGGPTQADVLFTPDDVVAEIGDLAIEKALRVRRAVDGGGA
                     ARDAIDALVRARRPAGG"
BASE COUNT          652 a         1805 c         1728 g          631 t
ORIGIN      
        1 cgcgcttcgt gaggccaacc gccttctccc gcacgctcgg cggcatggtc gggaaggtgt
       61 ggaagtgcag cggcagggag acctcgctca ccgcctgcca gaggggctcc cacaccggat
      121 gccacatcgg ctccatgtcc cacgagcacg agagctcgag cccgcgcagg cccatcttcg
      181 ccacccggtg gatctcgcgg accgcggcgg ggatgtcgcc gtagggcagg cacgccaggc
      241 ccacgtgccg ctcggggtag tggcggcaga agtcgaggag ccagtcgttg tagatgcgga
      301 acatctccgc ggccgcctcg tgatcgccga gccgcgtggc cgcgccgagg atgccgaaga
      361 tcacctccgc ctgcaccccg tcgcgctcca tgtccgccac gcgcagatgc ggatcggtcg
      421 gccgccggat ccccttgcgg ccatcgtcat agaggccggt cgacgccatg acgtccgcgc
      481 ggtagtggac gcccgggacg tacttctgcc ccgacggccc gaccccgccc gcgaggccga
      541 aggcggcgcc gttgcgcgac gtccaccgcg ggccgtcggg cccgtcggtg acgtagggca
      601 tgcggtcgcg cagggccgcg gtggcgttgg ccgtgaacag gtccggcggc atccacggca
      661 tgtcgatgtg gcagtcggcc gagattcgcg tgtattccat gggtgccctc ctccggcgcg
      721 gccatcataa tcccttccgc cccggcagtg ctatggtaag cctctccaag gaggtgtgag
      781 catgaccatg aagacggtcg tcgcgctgcc cggtgaaggg atcggcatcg aggtcgtcga
      841 cgccacgtgc gacctgctga tgggcaccgg cctgccgctc aagatcctca ccccgccgca
      901 gggctcgccc ctgcccgagg agaccaagcg cctggcccgc gaggccgacg gcgtgctctt
      961 cggggccgcc ggcccgtcca cctcgccggt ggtctcctgg ctgcgctggg agatggcggc
     1021 ctgggccggg gtgcggccga tccgcttctt ccccgggatg cgctcgcccc tcaccgaccc
     1081 cgacggcatc gactacgtgc tcctgcgcga gaacagcgag ggcctctacc ccgggcgcga
     1141 gggtgacctg gccgacctag tccggctgat gccgaacctg agcgaccgga ccgggcggac
     1201 ggtgagccag ttcggcaccg agggccgctt cgcggtcaag gtggtcacgc cccgcggggc
     1261 ggagcgcatc gcccgcttcg cgtgcgacct ggcccgcaag cggcaggcgc gcggcaagcc
     1321 cggcaaggtg acgctggtga ccaagtccaa cgtgctgccc aagaccgatg ggctctacga
     1381 cgagatcgtc ggccgcatcg tgcgcgaggc cggcctcccc ttcgagcact tccacgtcga
     1441 cgacgcggcc cgccgcctgg tgcgcttccc caaggcgatg gacgtcgtgc tctgcatgaa
     1501 cctctacggc gacatcctgt ccgacctggg cgcggagacc gcgggcggcc tcggcctcgc
     1561 cccgtccggc aacttcggcg acaactgggc ctacttcgag tcggtgcacg gctcggcgcc
     1621 cgacatcgcg ggcaagggca tcgccaaccc gaccgccacc atcctctcgg ccgccctcat
     1681 gctcgagcac atgggcctcg gcgccgaggc ccagcgcctc gaggccgcgg tcggccgcgt
     1741 ctatcgcgac ggcaaggtgc tcaccccgga ccagggcggc agcgcccgca cgcgcgacat
     1801 ggcgcaggcg gtgctggccg cgtaccgcta gcgggcgccc gccgatgagc gactgggacg
     1861 tcatcgtggt cggcgcgggc aacgcggccc tcgccgcggc ggtctccgcc cgcgagcagg
     1921 gcgcggggcg cgtgctcgcg ctggagaagg cgccgcgcgc gctgcgcggc ggcaacaccc
     1981 actacagcgg gggcctcctg cgcatcgcct tcgatcgccc cgacgacctg cgtccgctcg
     2041 tgccggacgc cgagcgagag gtgcccggct tctacgcggg cgtcgagccg tacccgcacc
     2101 gcctcttcat ggccgacctg ctgcgggtga ccgacgggcg gaccgatccc gagctggcca
     2161 agctgctgat cggccgctcc cacgacaccg cgtgctggat ggcccgccag ggcatcgaga
     2221 tggagccggc ggtctcgctc tcggcggtga aggtcggcga cacctacaag tggtcgcccg
     2281 gcgcggtgat ccgcgcgcgg cacgagggcg tcggcctctc cgcgatgtgg ttcaagatcg
     2341 cggagcgcga aggcgtcgag gtgcgctacg acaccgcggc ggagcggctg ctgcaggacc
     2401 ggagcgggcg cgtcaccggg gtggccgccc gcggccccaa cgggctcgag gagctatccg
     2461 cgcgcgcggt ggtgctgggc tgcggcggct tcgaggccaa tccggagtgg cgggcgcgct
     2521 acctggggcg gccgtgggat ctcgccaagg tgcgcggcac gcgctacaac accggcgacg
     2581 gcctgcgcat ggcgatggag gtgggcgccc tgccccacgg ccagtggacc ggctgccact
     2641 ccacgcccat cgacgcggac gcgcctccgc acggcgaccg caagctcacc gacaagacca
     2701 accgcctctc gtacccctat ggggtgctcg tcaacacgcg cggccagcgc ttcttcgacg
     2761 agggggagga cttccagttc tacacgtacg ccaagctcgg cgggatcatc ctcaacgagc
     2821 cgggcggcgt cggctggcag atcttcgacg ccaaggtcac gcatctcctg gaagggcgct
     2881 accagaccgg caccccggtg gtggcgcaca ccctggaggg cctggtggag aagctcccgc
     2941 tcgatcaggc ggcctgccgg cgcgccctcg aggcctacaa cgcggcggtc acgccgggcc
     3001 gcttcgatcc caccgtacgc gacggcctcg ccacccgcgg gctcacgctg cccaagtcca
     3061 actgggccca gcgcctcgac accccgccct actcggccta cccggtgacc ggcggcatca
     3121 ccttcacgtt cggcggggtg cgcgtcacca atcgcgccca ggtggtgagc atgagctggt
     3181 cggtcattgc cggcctctac gcctgcgggg agatggtggg cgggctcttc cacggcaact
     3241 atcccggcgg caccggcctc atgtcgggcg cggtgttcgg tcgcctggcc ggcgccgagg
     3301 cggccggcgc ctgacccggc cagcggagga tcgcgcgtga cgagcaagcc aaggatcgga
     3361 ttcgtggggc tcggcaccat gggcgggccg atggcgaggc ggctcgtcgc gagcggctac
     3421 gaggtcgccg gccacgaccc gagccccgat cgcgcgcgcc aggccgccgc tgcgggcgtc
     3481 accctcgagg cctccccggc cgccgcggcg aaggccgcgg aggtcgtgct gtcgagcctg
     3541 ccggatcccg ccgcggtccg tcaggcctac ctgggtcccg acggggtgct ctcgacggcc
     3601 cgcccgggca ccgtcctcgt cgacatgagc acgatcgatc cggacacctg ccgcgaggtc
     3661 gcggccgcag cccggaccgg cggcatcgac tgcctcgacg cgccggtgag cggcgggccg
     3721 gtcgaggcgg gcaacggcaa gctcgtcttc ctgctcgggg gcgacgcggc ggtgctcgag
     3781 cgcgtccggc cgctgctgct cacgctcggc accgagctcc accacatcgg cccgctcggc
     3841 tcgggcctcg tggtcaagat cgtcaacaac atgatgtcga tggccaacgt ggcggtggcg
     3901 gcggaggcga tgaccctcgg ggtgaaggcg ggcgtggatc cgcagcgcct cttcgacatc
     3961 ctctcgacga gcggcggccg ctcgcaccac ttcctcaagc gcttccccaa cgtgctggcc
     4021 ggcgacttca cgccgaactt cagcatcggg ctggcgcgca aggacgtgtc gctcgcgctc
     4081 gcgatggcgg cgcgtctcga gatgccggtg ccgctcgcgt ccatcgtgcg tcaggtctac
     4141 gaggccgccc acgcccaggg attcggccag ctcgacatgg cgggcgtgac ccggctctac
     4201 gagcagtgga ccggggtggc ggtgcgcggc gcggcggccc gcgacgccga gaagcccacc
     4261 gccggctagg ccgcggccgc ctcagccgcc cgcgggacgc cgggcgcgga ccagcgcgtc
     4321 gatcgcgtcg cgggccgccc cgcccccatc gaccgcgcgg cgcacccgca gggccttctc
     4381 gatcgcgaga tcgccgatct ccgcgaccac gtcgtcgggc gtgaagagca cgtcggcctg
     4441 cgtgggcccg cccgagccct cggtgaggtt cagcaggtcg tggcccacga ccagcagcgt
     4501 gccgccgggc cgcaccgcgg aagcggcgtg ggccagcgcg acccggcgct cgtgggcggg
     4561 cagctgcagg tacatcacgg tgacgaggtc gtaggcccgt ccgggcggcg cccaggtcac
     4621 cgcgtccgcc tcgacccact cgatctcgac gccggagccg gcggccaggt cgcgggccgc
     4681 ccgcagcgcc acctcggaga agtccacccc ggtgactcgc cagccgcgcc gcgccagcca
     4741 caccgcgttg cgaccggccc cacacgccac gtcgagcgcg cgccccggcg gcaggacttc
     4801 cgtctcggcg atcagg
//
LOCUS       DATMCV010000940         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_649490, whole genome shotgun sequence.
ACCESSION   DATMCV010000940 DATMCV010000000
VERSION     DATMCV010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649490"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VNU03_21505"
     CDS             <1..302
                     /locus_tag="VNU03_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77113.1"
                     /translation="ANFLGEGVLIAAVNARLPEARLLARAIRWGVLLFASATAITHLG
                     IGKEMVLVAFGITFGGLIFALALAFGLGGRGLARQILERHLRSREPHPRETLTHL"
     gene            308..>2083
                     /locus_tag="VNU03_21510"
     CDS             308..>2083
                     /locus_tag="VNU03_21510"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWP77114.1"
                     /translation="MIDADVLFQQAAAALRHERRASYRLQLGSALGFDEVAALGAYLE
                     ALGIGEVYLSPCFRCGPGSSHGYDVTDHNAFNPELGSAATFDRMAAGLAARGLGLILD
                     VVPNHMGIAGDANPWWLDVLENGPASPRAEFFDIDWAPAKAELRNRVLLPILPDQYGR
                     VLESQQLALEFADGAFHLRYAGARLPITPRTYPQILTDGLEPVAQRLGPEDAGLQELR
                     SILTAIEHLPGRTETDPRRVEERLREKEIVKRRLMALAKESADIREHIEETVRRFNGV
                     AGDPASFDALDGILSAQTYRLADWRVAGDEVNYRRFFDVSHLAAIRMERRNVFDATHQ
                     LILRLVGEGKVTGLRIDHPDGLYAPGEYFLRLQEGALIATARRLVPELGAPEAEGLAA
                     LYRTRAADRAASLEARPLWIAAEKILGPDEPLPEWWAVAGTTGYDFLAAVNGLFVDRG
                     TSRQMTALYSRVAGDGARMADLTYGAKRLIMQVSMASEINQLGYTLDRMSERDRLARD
                     FTLPSLGRAIREVVAAFAVYRTYVGDDGSGTPGPRDRGYIESAVAEAKRRNPTVNVSI
                     FDFVRDVLLLRHPDRSGPEERAARRH"
BASE COUNT          280 a          804 c          711 g          288 t
ORIGIN      
        1 tggcgaactt cctcggcgag ggcgtcctca tcgcggcggt caacgcccgc ctgcccgagg
       61 cgcgactcct ggcccgggcc atccgctggg gcgtgctcct cttcgcgagc gccaccgcca
      121 tcacccacct gggcatcggc aaggagatgg tgctggtcgc gttcgggatc accttcggcg
      181 ggctgatctt cgccctcgcg ctggccttcg gcctcggcgg gcgcgggctg gcccgccaga
      241 tcctcgagcg ccacctccgc agtcgcgagc cgcatccgcg cgagaccctg acccacctct
      301 aggccgcatg atcgacgccg acgtgctctt ccagcaggcg gcggcggcgc tgcgccacga
      361 gcgccgcgcg agctaccgcc tgcagctcgg gagcgcgctc ggcttcgacg aggtcgcggc
      421 cctcggtgcc tacctggagg cgctcggaat cggcgaggtg tatctctccc cctgcttccg
      481 ctgcggcccc ggcagctccc acggctacga cgtcaccgac cacaacgcgt tcaatcccga
      541 gctggggagc gcggccacgt tcgaccggat ggccgccggg ctggccgcgc gcgggctcgg
      601 cctgatcctg gacgtggtgc cgaaccacat gggcatcgcg ggcgacgcca atccctggtg
      661 gctcgacgtg ctcgagaacg gcccggcctc gccacgggcc gagttcttcg atatcgactg
      721 ggccccggca aaggccgagc tgcgcaaccg cgtgctcctg ccgatcctcc ccgaccagta
      781 cggccgggtg ctcgagtcgc agcagctcgc gctcgagttc gcggacggtg ccttccacct
      841 gcgctacgcg ggcgcgcgcc tgcccatcac cccccggacc tacccgcaga tcctgaccga
      901 cgggctcgag ccggtcgccc agcgcctggg cccggaggac gcggggctcc aggagctgcg
      961 cagcatcctg accgcgatcg agcacctgcc cgggcgcacc gagaccgatc cccgtcgggt
     1021 cgaggagcgg ctgcgcgaga aggagatcgt caagcggcgg ctgatggcgc tcgccaagga
     1081 gtccgccgac atccgcgagc acatcgagga gacggtgcgg cgcttcaacg gggtcgcggg
     1141 cgatcccgcc tccttcgacg ccctcgatgg catcctctcc gcgcagacct atcgcctggc
     1201 cgactggcgg gtggccggcg acgaggtgaa ctaccgccgg ttcttcgacg tgagccacct
     1261 ggccgcgatc cgcatggagc ggcgcaacgt gttcgacgcg acccaccagc tgatcctgcg
     1321 tctggtcggc gagggcaagg tcaccgggct gcgcatcgat cacccggacg gcctctacgc
     1381 gcccggcgag tattttctcc ggctgcagga gggcgccctg atcgcgaccg cgcggcgcct
     1441 ggtgcccgag ctgggcgccc ccgaggcgga ggggctcgcc gccctctacc ggacccgcgc
     1501 cgcggaccgg gccgccagcc tggaggcgcg gcccctctgg atcgcggccg agaagatcct
     1561 cggcccggac gagcccctgc ccgagtggtg ggcggtggcc ggcaccaccg gctacgactt
     1621 cctcgccgcg gtgaacggcc tcttcgtcga ccggggcacc tcccgccaga tgaccgcgct
     1681 ctactcgcgg gtggcgggcg acggcgcccg catggccgat ctcacctacg gagcgaagcg
     1741 gctcatcatg caggtctcga tggcgagcga gatcaatcag ctcggctata ccctcgaccg
     1801 catgtccgag cgcgaccgcc tcgcccgcga cttcaccctg ccgagcctcg gccgcgcgat
     1861 ccgcgaggtg gtggccgcct tcgcggtcta ccgcacctac gtgggcgacg acggcagcgg
     1921 caccccgggc ccgcgcgacc gcggctacat cgagtccgcg gtcgcggagg ccaagcgccg
     1981 caaccccacc gtgaacgtgt cgatcttcga cttcgtccgc gacgtgctcc tgctgcgcca
     2041 tccggaccgc agcggccccg aggagcgcgc cgcgcgacgc cac
//
LOCUS       DATMCV010000941         3768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_48970, whole genome shotgun sequence.
ACCESSION   DATMCV010000941 DATMCV010000000
VERSION     DATMCV010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48970"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VNU03_21515"
     CDS             complement(<1..561)
                     /locus_tag="VNU03_21515"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWP77115.1"
                     /translation="MAASPTAADEPDPFAPILHALDYLAVDYPGAVKDGKVLDQGEYA
                     EQVEFATSVRGLIAGLPARPERAALEATAAGLLGAIRDKRPGEEVAAISGDLRRRIID
                     LYDVRVTPRQVPELAPAAADFTTHCAVCHGATGHGDGPGAKGLTPPPADLTDAARMGE
                     HSLFGLYNTITLGIKGTAMTGFAPLGE"
     gene            complement(603..833)
                     /locus_tag="VNU03_21520"
     CDS             complement(603..833)
                     /locus_tag="VNU03_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77116.1"
                     /translation="MAPGRPGLRLRALELAERGKLDEALAVARRAPGAPARMIAAGLA
                     ERPGTATAAMEAAAQAGVGKLAIAVSPREAQR"
     gene            complement(1051..1224)
                     /locus_tag="VNU03_21525"
     CDS             complement(1051..1224)
                     /locus_tag="VNU03_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HWP77117.1"
                     /translation="MIDDSGEAAATREVTTGPAVRIVSTELFRGRREIVIVHRGQEYR
                     LHITKADKLILTK"
     gene            complement(1221..1700)
                     /gene="bfr"
                     /locus_tag="VNU03_21530"
     CDS             complement(1221..1700)
                     /gene="bfr"
                     /locus_tag="VNU03_21530"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HWP77118.1"
                     /translation="MKGHDQVVTLLNEILTAELTAINQYFIHARMCENWGYKRLWKKL
                     REESIGEMRHADRLIERVLYLEGMPNVQRLGKVNVGQTVPEQLRLDLELERSAVAALN
                     AGVELCRGAGDNGSRDLLEDILKSEEGHIDWIEEQMELIKQVGEAHYLAQQIKEEDA"
     gene            complement(1721..1915)
                     /locus_tag="VNU03_21535"
     CDS             complement(1721..1915)
                     /locus_tag="VNU03_21535"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWP77119.1"
                     /translation="MILCLCRGVSEHTVHAVISAGARSVDEIERSCGAGGDCGACSEA
                     LECLVERVRERHYSGATAAH"
     gene            complement(2094..2630)
                     /locus_tag="VNU03_21540"
     CDS             complement(2094..2630)
                     /locus_tag="VNU03_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77120.1"
                     /translation="MIVYHVAIETAGDYVARREPHRRVHLDRLMALRGQGLIIGGGPS
                     PDGRGADIFYRVQQPDDLRGLVETDPYFTGGAWSGYRSRSFAQFLEPWELPPLVTDGS
                     RPVTIAEGGAPDVEMASFALIEARGAGRMAFGGFFPGGETLAIMRAPDADKALEELRA
                     SGFWQEGSLRGRPLLHVL"
     gene            complement(2627..3385)
                     /locus_tag="VNU03_21545"
     CDS             complement(2627..3385)
                     /locus_tag="VNU03_21545"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77121.1"
                     /translation="MKLTGKVALITNVSHFMGPAITEEFCREGASVALHDREPALIKP
                     FATIAERLGRDVLALGGDLSRSAEAERAVEAVVARFGRLDVLVNNNAHPPSGSPVEQI
                     TDAAWREMQSRLLDEPFFCLRAALRVMRPAGRGKIINMSSAAAFPGLPNYAAYTAARA
                     GVNGLTKAVGREVAKDGIQVNAIAQNYVENPTYFPPALVADAAKRERMIRNIPAGRLA
                     RSEESARLAVYLASEDADFFVGQVIPFAGGWVTP"
     gene            3467..>3768
                     /locus_tag="VNU03_21550"
     CDS             3467..>3768
                     /locus_tag="VNU03_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWP77122.1"
                     /translation="MAEELLQSFKARYPFSLDDFQLRAISAILDGKSVIVSAPTGSGK
                     TLVAEFAIHSAQHRGRRIAYTTPLKALSNQKYADFGRQWGAENVGILTGDVKVNTRA"
BASE COUNT          506 a         1317 c         1397 g          548 t
ORIGIN      
        1 ctcgccgagg ggcgcgaagc cggtcatggc ggtgcccttg atgccgaggg tgatcgtgtt
       61 gtagaggccg aagaggctgt gctcgcccat gcgggccgcg tcggtaagat cggcgggcgg
      121 cggggtcagg cccttggcgc cgggcccgtc gccgtggccg gtggcgccgt ggcagaccgc
      181 gcagtgggtt gtgaagtcgg cggcggcggg cgccagttcg gggacctgcc gcggggtcac
      241 gcgcacgtcg tagagatcga tgatgcggcg gcggagatcg cccgagatcg ccgcgacctc
      301 ctcgccgggg cgcttgtcgc ggatcgcgcc gaggaggccg gccgcggtgg cctcgagcgc
      361 ggcccgctcg gggcgggcgg ggaggccggc gatcaggccg cgcaccgagg tggcgaactc
      421 gacctgctcg gcgtactcgc cctggtccag gaccttgccg tccttgaccg cgcccgggta
      481 gtcgaccgcc aggtagtcga gggcgtgcag gatgggcgcg aaggggtcgg gctcgtccgc
      541 cgcggtgggg gacgcggcca cgcccaccag caggaagacg agcgcgaggg cccggcccgc
      601 ggtcagcgct gtgcctcccg cggcgagacc gcgatcgcga gcttgccgac cccggcctgg
      661 gccgcggcct ccatcgccgc ggtggcggtc cccggccgct ccgcgagccc ggccgcgatc
      721 attcgagcgg gtgcgcccgg cgcgcgacgg gccaccgcga gggcctcgtc gagcttgccg
      781 cgctcggcca gctcgagcgc gcgcaggcgg agcccgggtc gccctggcgc caccagaagg
      841 cgaggcgctc cacgaccacg gccagggcca cgatcgagca ggcgagcagg ggaatcatga
      901 cgggaccacc cttggcgagt gtctggacga gcgttccgat ctgtgacacg agcccctcgc
      961 tgccggcttc gtctcggcga cggccggctc tggtgcctcg ctgccgggag cgggaggccg
     1021 ccccgaaggt ggctggcgtt ccgtgaacgg ctacttggtc aggatgagct tgtcggcctt
     1081 cgtgatgtgg agccggtact cctggccccg gtggacgatg acgatctcgc gccggccccg
     1141 gaagagctcg gtgctcacga tgcgcacggc agggccggtg gtcacctcgc gggtggcggc
     1201 cgcctcgccg gagtcgtcga tcacgcgtcc tcttccttga tctgctgggc gaggtagtgg
     1261 gcctcgccca cctgcttgat cagctccatc tgctcctcga tccagtcgat gtgcccctct
     1321 tccgacttca gaatgtcctc gagcaggtcg cggctgccgt tgtcgcccgc gccgcggcag
     1381 agctccaccc ccgcgttgag cgcggccacc gcgctccgct ccagctcgag gtcgagccgg
     1441 agctgctcgg ggacggtctg gcccacgttc accttgccga ggcgctgcac gttgggcatg
     1501 ccctcgaggt agaggacgcg ctcgatcagc cggtcggcgt ggcgcatctc accgatcgac
     1561 tcctcgcgga gcttcttcca caggcgcttg tagccccagt tctcgcacat gcgcgcgtgg
     1621 atgaagtact ggttgatggc ggtcagctcc gcggtgagga tctcgttgag caaggtcacc
     1681 acctggtcgt gtcccttcat ggctcgcctc cgtgaggacg ttagtgggcc gcggtcgcgc
     1741 cactatagtg cctctcacgg acacgctcga ccaggcattc gagcgcctcg ctgcaggccc
     1801 cgcagtcgcc ccccgcgccg cagctccgct cgatctcgtc caccgagcgg gcgcccgcgg
     1861 agatgacggc gtggacggtg tgctcggaga cgccacggca gagacagagg atcacgggcg
     1921 ggccttccgg gagccgagcg acaacctgtt cattcgggct gctttccgtt cacctttagg
     1981 gtggtgatag ccagcggatt aggtatacct aaacagaaag caccctgtca accgggaaaa
     2041 atggcacgcc ccgggcgggg cccggggggc tggcggcggg cggctgcggc ggcctagagc
     2101 acgtgcagga gggggcgccc gcgcagcgag ccctcctgcc agaagccgct ggcgcgcagc
     2161 tcctcgagcg ccttgtccgc gtccggggcg cgcatgatcg ccagggtctc gccgccgggg
     2221 aagaagccgc cgaaggccat ccggccggcg ccgcgcgcct cgatcagggc gaaggaggcc
     2281 atctccacgt cgggcgcgcc gccctccgcg atggtgaccg ggcgggagcc gtcggtcact
     2341 agcgggggca gctcccaggg ctcgaggaac tgggcgaagc tccgcgaccg gtagccggac
     2401 cacgcgccgc ccgtgaagta ggggtccgtc tccacgagcc cccggaggtc gtcgggctgc
     2461 tggacgcggt agaagatgtc ggcgccgcgc ccgtcgggcg agggcccgcc cccgatgatg
     2521 aggccctggc cgcgcagggc catgagccgg tcgaggtgca cgcgccggtg cggctcgcgc
     2581 cgggccacgt agtcgcccgc ggtctcgatc gccacgtggt agacgatcac ggcgtcaccc
     2641 agccgcccgc gaaggggatg acctggccca cgaagaagtc ggcgtcctcc gaggccaggt
     2701 agaccgcgag cctagctgac tcctcgctgc gggcgagccg gccggccggg atgttccgga
     2761 tcatgcgctc gcgcttcgcc gcgtccgcca cgagcgcggg cgggaagtag gtcgggttct
     2821 cgacgtagtt ctgcgcgatc gcgttcacct ggatgccgtc cttcgccacc tcgcggccga
     2881 ccgccttggt gagcccgttc acgccggcgc gcgccgcggt gtaggccgcg tagttgggca
     2941 ggccggggaa ggcggccgcg gaggacatgt tgatgatctt gccgcgcccg gccggccgca
     3001 tcacccgcag cgcggcgcgc aggcagaaga acggctcgtc gagcaggcgg gactgcatct
     3061 cgcgccaggc cgcgtcggtg atctgctcga ccgggctgcc ggagggcgga tgcgcgttgt
     3121 tgttgaccag gacgtcgagg cgcccgaagc gcgccaccac cgcctccacc gcgcgctccg
     3181 cttccgccga gcggctcagg tcgccgccca gcgcgagcac gtcgcggccg agccgctccg
     3241 cgatggtggc gaacggcttg atcagggccg gctcgcggtc gtgcagcgcc accgaggcgc
     3301 cctcgcggca gaactcctcg gtgatggcgg gccccatgaa gtggctcacg ttggtgatga
     3361 gggcgacctt gccggtgagc ttcatggtcc ccgagcctac gcggggcttc gcggccacgc
     3421 aaggccgtgg tacggtgagg gcgcgggcaa gggaggtgcc gcggccgtgg ccgaggaact
     3481 gctccagtcg ttcaaggccc gctacccgtt ttcgctcgac gatttccagc tccgcgccat
     3541 ctccgccatc ctcgacggca agtcggtgat cgtctcggcc ccgaccggct ccggcaagac
     3601 cctggtggcc gagttcgcga tccattccgc gcagcaccgc gggcgccgca tcgcctacac
     3661 cacgccgctc aaggcgctct ccaaccagaa gtacgccgac ttcggccggc agtggggcgc
     3721 ggagaacgtc ggcatcctca ccggcgacgt gaaggtgaac acgcgggc
//
LOCUS       DATMCV010000942         4375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_117565, whole genome shotgun sequence.
ACCESSION   DATMCV010000942 DATMCV010000000
VERSION     DATMCV010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117565"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VNU03_21555"
     CDS             complement(<1..494)
                     /locus_tag="VNU03_21555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HWP77123.1"
                     /translation="MRTVPGPEASVADGVAPHGVAWRIHRERVLLAGWGRAILLQLAH
                     PMVARGVAEHSGFATDRWGRVRRLHRTLHAMLALTFGPAEGAGAAAARINAIHDRVHG
                     RLDHAAGGEHAGAPYSAHDPALLAWVHATLLDSFLGAYRLFVGPLDTVEADRYCLESS
                     GIEAR"
     gene            582..800
                     /locus_tag="VNU03_21560"
     CDS             582..800
                     /locus_tag="VNU03_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWP77124.1"
                     /translation="MRRSTLPLVAALLLAGLALLFPIASPVAALEVGQKAPDFTLAAP
                     GGKQVKLSDLTAKGPVVIFTFIQAFTGT"
     gene            810..1139
                     /locus_tag="VNU03_21565"
     CDS             810..1139
                     /locus_tag="VNU03_21565"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWP77125.1"
                     /translation="MLSFEGAMPQFEAAGAQVVGVSADHVATLEAWGKQNNVHYLLLS
                     DFRRTMLPAYDALVTDDKSPIYRYAKRAYYVIDKNGVVRWIKVEANPLDLLDPAEVLK
                     ALKESGV"
     gene            1238..1693
                     /locus_tag="VNU03_21570"
     CDS             1238..1693
                     /locus_tag="VNU03_21570"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1367..1369,aa:Sec)
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWP77126.1"
                     /translation="MGVQVYESPKPAPAFALPALDGRSVRLEDYRGKALLLFFWATWU
                     PDCREELPSVNRLYQEMKGQGLEVLLISFRESADLVKRTVSERGYVATVLLDASGDVT
                     GRQYGVWGPPTAYLVDREGRLVGRMVGAKDWSGAPARDLLRAFVTAPPR"
     gene            complement(1713..2285)
                     /locus_tag="VNU03_21575"
     CDS             complement(1713..2285)
                     /locus_tag="VNU03_21575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HWP77127.1"
                     /translation="MPRLLAVVGAVTRPGRLDAAVRFAADCAEALPEVSATVLSLAGH
                     RVSFADGRPLEGYPDDTAQIVSAIAAADAVLLASPVYRASFTGALKNLLDLTPLEALR
                     AKPVGIVAMGATSHHYLGVDWQLRSVLAWFGALVVPTSVYLESGHFQDGRLADAAAAA
                     ALTDLVRALVAMTGHARDQAGPPPLAAGRG"
     gene            complement(2290..3384)
                     /locus_tag="VNU03_21580"
     CDS             complement(2290..3384)
                     /locus_tag="VNU03_21580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77128.1"
                     /translation="MAIELIGMIGVRPEGADGAAVHVIGGEIDLGWVRDFSRAHEGAG
                     FDKVLVGYTATSADGFLVSSYAAAHTERLGYLIAHRPGFVAPTLAARKAATLDQISGG
                     RVSLHIITGGSDADQARDGDYVDHDTRYRRTDEYLGLMRRMWTEDRPFDHAGEFYRVT
                     KAGSDVKPVQKPHVPLYFGGASEAAHRIGARHCDVYMLWGEPLAAIRDRIAEVRAAAA
                     GVGRDPRELRFSVSLRPIIAPTEAAAWAKARGILERVNAKQGDRTLPLPEAVGARRLV
                     DFAQQAEVHDKRLWTPIAAAVGGAGNTTALVGTPEQVAESLLDYYDLGVSTLLIRGFD
                     PLADAAEYGRELIPLVRAGAARRDRDRPAA"
     gene            complement(3384..>4375)
                     /locus_tag="VNU03_21585"
     CDS             complement(3384..>4375)
                     /locus_tag="VNU03_21585"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWP77129.1"
                     /translation="GAPVRTGVRVDAIRRGPGADGFRLETSHGIVVASNVVVATGPYQ
                     EPVIPALASALPPRIAQAHSRAYRNPAELPPGAVLVVGSGASGCQIVEDLLATGRRVF
                     LSVGRHRRFPRRYRGRDMFWWMERMGALDQTLEERPDARERPNPLVTGVGGGHEINLR
                     FYAARGVVLLGHVDAIEESRLRLADDLDAQLTRGDEGVAVFTRAVDAYVERAGLSAPE
                     EPAPAPPPPLPTSAPTRALDLDAESVTSVIWATGFRRDFGWIHLPVLDERGEPIHRRG
                     VTSCPGLYFLGLPWLHTLKSSVLCGVGRDADHLADCIASRAAAPALAPTLRRP"
BASE COUNT          606 a         1585 c         1623 g          561 t
ORIGIN      
        1 cgggcctcga tcccgctcga ctccaggcag tagcggtcgg cctcgacggt gtcgaggggg
       61 ccgacgaaga gccggtaggc gcccaggaac gagtcgagca gcgtggcgtg cacccacgcg
      121 agcagggccg ggtcgtgggc gctgtacggg gcgccggcgt gctcgccgcc cgcggcgtgg
      181 tcgaggcggc cgtggacgcg atcgtggatc gcgttgatcc gggccgcggc cgcccccgcg
      241 ccctcggcgg gaccgaaggt gagcgccagc atggcgtgga gcgtccggtg caggcgccgc
      301 acccgccccc accggtcggt ggcgaagccg ctgtgctcgg ccaccccccg cgccaccatg
      361 gggtgggcga gctgcagcag gatcgcccgg ccccatcccg cgagcagcac ccgctcccgg
      421 tgtattctcc aggcaacccc gtggggcgcg accccgtcgg cgacggaagc ctccggcccc
      481 ggcacggtgc gcatgagcgc attatgcgcg cggccgggaa acttcgggag cccgccgggg
      541 gttctgtcag agtccaccca cactccggaa aggagactgc catgcgtcga agcaccctgc
      601 cgctggtcgc agccctgctg ctggccggcc tggccctgct gttcccgata gcgtccccgg
      661 tcgccgccct ggaagtcggc cagaaagcgc ccgacttcac cctggcggct cccggcggca
      721 agcaggtcaa gctttccgac ctgaccgcca agggccccgt ggtcatcttc accttcatcc
      781 aggcgttcac cggcacctga accaaggaac tgctcagctt cgagggggcc atgccccagt
      841 tcgaggctgc gggcgcccag gtcgtgggcg tgagtgccga ccatgtcgca acgctggaag
      901 cctggggcaa gcagaacaac gttcactacc tcctgctctc cgatttccgc cgcacgatgc
      961 ttccggccta tgacgcgctg gtcaccgacg acaagagccc gatctaccgc tacgccaagc
     1021 gcgcgtacta cgtgatcgac aagaacggcg tcgttcgctg gatcaaggtc gaggccaatc
     1081 ccctcgatct gctcgatccg gcggaggtgc tcaaggcgct gaaggagtcc ggcgtctgat
     1141 gatcgcgagg atcgccgcgg ggagcgtgat cgcggcggca ctctgggcca cggtcgcggc
     1201 ggcgcagcct gccgccgcgc cgtcggcctg ggagaagatg ggcgtgcagg tgtacgagtc
     1261 gccgaagccg gcgcccgcct tcgcgctgcc cgcgctggac ggccgcagcg tgcggctcga
     1321 ggactaccgg ggcaaggcgc tcctgctctt cttctgggcc acctggtgac cggactgccg
     1381 ggaggagtta ccttccgtca accggctgta ccaggagatg aaggggcagg ggctcgaggt
     1441 gctcctgatc agcttccggg agagcgccga cctcgtgaag cgcaccgtga gcgagcgcgg
     1501 ctacgtggcg acggtgctgc tcgacgccag cggcgacgtc accggtcgcc agtacggagt
     1561 ctggggcccg cccaccgcgt acctggtgga tcgagagggg cgcctggtcg gccgcatggt
     1621 cggcgccaag gactggagcg gcgcacccgc gcgcgacttg ctccgcgcct tcgtcaccgc
     1681 cccgccccgc tgaccgctcg cccgccggcc ggctagccgc gcccggccgc caatggaggt
     1741 ggtcccgcct gatcgcgggc gtggcccgtc atcgccacca gcgcgcgcac gagatcggtg
     1801 agcgcggccg ccgcggcggc gtcggcgagc cggccgtcct ggaagtggcc ggactcgaga
     1861 tagacgctgg tcggcaccac cagcgctccg aaccaggcca gcaccgaccg cagctgccag
     1921 tccaccccga ggtagtggtg cgacgtggct cccatcgcca cgatgcccac cggcttggcg
     1981 cggagcgcct cgagcggggt caggtcgagc aggttcttga gcgcgccggt gaacgaggcg
     2041 cggtagaccg ggctcgcgag cagcaccgcg tccgccgccg cgatcgcgct cacgatctgc
     2101 gcggtatcgt ccggatagcc ctcgagcgga cgcccgtcgg cgaacgacac gcggtgcccg
     2161 gccaggctca gcacggtggc cgacacctcg ggcagcgcct ccgcgcaatc ggccgcgaac
     2221 cgcaccgccg cgtcgagccg gccgggccgg gtcaccgcgc cgaccaccgc gagcaggcgg
     2281 ggcatcggct caggccgccg gtcgatcccg atcgcgccgg gccgccccgg cgcgcaccag
     2341 cgggatcagc tcgcggccgt actcggccgc gtcggcgagc ggatcgaagc cgcggatcag
     2401 cagcgtggag acgcccaggt cgtagtagtc gagcagcgac tcggccacct gctccggcgt
     2461 gcccacgagc gcggtggtgt tgccggcgcc gccgaccgcc gccgcgatcg gcgtccagag
     2521 ccgcttgtcg tgcacctcgg cctgctgcgc gaagtcgacc aggcgccgcg cgccgaccgc
     2581 ctcgggcagc ggcagcgtgc ggtcgccctg cttggcgttg acgcgctcga ggatcccccg
     2641 ggccttggcc cacgcggccg cctcggtggg cgcgatgatc gggcggaggg agacgctgaa
     2701 ccgcagctcg cgcgggtccc ggccgacgcc cgcggcggcg gcgcgcactt ccgcgatccg
     2761 gtcgcggatg gcggcgagcg gctcgcccca gagcatgtag acgtcgcagt gccgggcccc
     2821 gatccggtgg gcggcctcgg aggcgccgcc gaagtagagc gggacgtgcg gcttctggac
     2881 cggcttgacg tccgagcccg ccttcgtcac ccggtagaac tcgcccgcgt gatcgaacgg
     2941 ccggtcctcg gtccacatgc ggcgcatgag cccgaggtac tcgtcggtgc gacggtagcg
     3001 cgtgtcgtgg tcgacgtagt cgccgtcgcg cgcctgatcg gcgtcgctgc ccccggtgat
     3061 gatgtggagc gacacccgcc cgcccgagat ctggtcgagg gtggccgcct tgcgcgcggc
     3121 cagggtcggc gccacgaagc cgggccggtg cgcgatcagg tagccgaggc gctcggtgtg
     3181 ggccgccgcg tagctcgaga cgaggaagcc gtcggccgag gtcgctgtgt agccgacgag
     3241 caccttgtcg aagccggccc cctcgtgggc ccgcgagaag tcgcggaccc agccgagatc
     3301 gatctcgccg ccgatcacgt ggaccgcggc gccgtccgcc ccttcgggcc tcacgccgat
     3361 catgccgatg agctcgatgg ccatcatggt ctcctgagtg tgggggcgag cgccggggcg
     3421 gcggcccgcg acgcgatgca gtccgccagg tggtcggcgt cgcggccgac cccgcagagg
     3481 acggatgatt tcagggtgtg cagccacggc agcccgagga agtagagccc cgggcacgag
     3541 gtgacgccgc gccggtggat cggctcgccg cgctcgtcga ggaccggcag gtggatccag
     3601 ccgaagtcgc ggcggaagcc ggtggcccag atcacgctgg tgacggactc cgcgtcgaga
     3661 tcgagggcgc gggtcggcgc cgacgtcggc aacgggggcg gcggcgcggg ggcgggctcc
     3721 tccggcgccg agaggccggc acgctccacg taggcgtcga ccgcgcgggt gaagacggcc
     3781 acgccctcgt cgccccgggt cagctgggcg tcgagatcgt cggcgagccg gaggcgggac
     3841 tcctcgatcg cgtcgacgtg gccgagcagc accaccccgc gcgccgcgta gaaccgtaga
     3901 ttaatctcgt ggccgccgcc caccccggtc acgagcgggt tgggccgctc gcgcgcgtcg
     3961 ggccgctcct cgagcgtctg gtcgagcgcg cccattcgct ccatccacca gaacatgtcg
     4021 cggccgcggt agcggcgagg gaaccggcgg tgacggccca ccgagagaaa cacccggcgg
     4081 cccgttgcga gcaggtcctc gacgatctgg cagccggacg cgcccgaccc gacgaccagc
     4141 accgcgccgg ggggcagctc cgcggggttg cgataggcgc gcgagtgcgc ctgcgcgatc
     4201 cgcggcggca atgcagaggc cagcgccggg atcaccggct cctggtaggg cccggtcgcc
     4261 accaccacgt tggaggcgac gacgatgccg tgcgaggttt cgagccggaa cccgtcggcc
     4321 ccgggccccc gccggatcgc gtcgacgcgc acaccggtgc ggaccggtgc tccgg
//
LOCUS       DATMCV010000943         2452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_252240, whole genome shotgun sequence.
ACCESSION   DATMCV010000943 DATMCV010000000
VERSION     DATMCV010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252240"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..153
                     /locus_tag="VNU03_21590"
     CDS             13..153
                     /locus_tag="VNU03_21590"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWP77130.1"
                     /translation="MCGMTVEVATARHQSDWAGRSVYFCCRHCKETFDAEPARYAAAL
                     AG"
     gene            206..1126
                     /locus_tag="VNU03_21595"
     CDS             206..1126
                     /locus_tag="VNU03_21595"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWP77131.1"
                     /translation="MPRPKLMSMREAVARFVPDGTSICMGAALEALIPFAAGHELIRQ
                     RRRDLTLIGPISDILFDQLIGAGCVRRVIAAWVGNVSAGLGYNYRRAVEQRIPAPIEV
                     QDHSNLTIAFALQAAALGAPYLPTRSLLGTDLPRANATFKEAADPWSGAPLLLVPAIA
                     PDVAILHVQRADEEGHAHVWGTLGITRPAALAARQVILVAEEIRPRERILGDPGLVLT
                     PALKVAAVVHQPFGAFPSPVQGHYGRHHDFYHRFHDESRTVEGFQRWLAHWVHEVPDW
                     AGFLSRLDAGVLDGLRVKRPQLSEPLDYGA"
     gene            1132..2247
                     /locus_tag="VNU03_21600"
     CDS             1132..2247
                     /locus_tag="VNU03_21600"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWP77132.1"
                     /translation="MKITAIEAVPLAIPLRPMNPPSPWTGGTRKQIFVRVHTDEGVSG
                     LGEAFAYGAPLAVVSVIEESLAPLIVGQDPLRIEAVVDLMHRGTMIYGRRGLGMFAIS
                     GIEIALWDLLGKVRGAPVYELLGGALRPRLPAYASLLRYETPDAVAGACRHFVAQGFR
                     MLKLHQTDVASVRAAREAVGPDVELMLDTNCPWTPFEAIAMARALAPYRLFWLEEPVW
                     PPEDYAGLARVAAATETPIALGENESTVHGFHEIVRQGAGDILQPSITKVGGISEFKK
                     IAAVAQAANLPIAPHSFYFGPGLAATLHVAATFGGPLPVEFPTGDQETPFLTRPIQAR
                     DGWLELPSGPGLGVEINEEALRRHPFVKNAAQPFVLR"
     gene            2256..>2452
                     /locus_tag="VNU03_21605"
     CDS             2256..>2452
                     /locus_tag="VNU03_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003320712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWP77133.1"
                     /translation="MDFTERELMVIAAAREIRDDDLVFVGMRLPLIAFAYAKRTHAPG
                     ATGLFENGIVRDQPSPETLLT"
BASE COUNT          332 a          942 c          822 g          356 t
ORIGIN      
        1 gccaaggacc ccatctgcgg catgaccgtg gaggtggcca ccgcccgcca ccagtcggac
       61 tgggcgggcc gctcggtgta cttctgctgc cgccattgca aggagacctt cgacgccgag
      121 cccgcgcgct acgcggcggc gctcgccggc tgacgatcca ctccctcgaa gcggccccgc
      181 ctccggcgtg gagtaagata cccgggtgcc tcggcccaag ctcatgtcca tgcgggaggc
      241 cgtcgcccgc ttcgtgcccg acggcacctc gatctgcatg ggcgccgcgc tcgaagcgct
      301 catccccttc gcggcgggcc acgagctgat ccgccagcgg cgacgcgacc tcacgctcat
      361 cgggccgatc tcggacatcc tcttcgacca gctcatcggc gcgggctgcg tgcggcgggt
      421 gatcgcggcg tgggtcggca acgtgagcgc cggcctcggc tacaactacc ggcgcgcggt
      481 cgagcagcgg atccccgctc ccatcgaggt ccaggatcac tccaacctga ccatcgcctt
      541 cgcgctgcag gccgccgccc tcggcgcgcc ctacctgccc acgcgctcgc tgctcggcac
      601 cgatctcccg cgcgcgaacg cgaccttcaa ggaggcggcc gatccgtggt cgggcgcccc
      661 gctcctgctc gtccccgcga tcgcccccga cgtagccatc ctccacgtgc agcgcgccga
      721 cgaggagggt cacgcgcacg tctggggcac tctcgggatc acccgcccgg ccgccctcgc
      781 ggcccggcag gtgatcctgg tcgcggagga gatccgcccg cgcgagcgca tcctgggcga
      841 tccgggcctc gtgctcactc ccgcgctcaa ggtcgccgcg gtggtgcacc agccgttcgg
      901 cgcctttccc tcgccggtgc agggccacta cgggcgtcac cacgacttct atcaccgctt
      961 ccacgacgag agccggacgg tggagggctt ccagcgctgg ctcgcgcact gggtgcacga
     1021 ggtcccggac tgggccggct tcctctctcg cctcgacgcg ggcgtcctcg acggcctgcg
     1081 cgtgaagcgc ccgcagcttt ccgagccgct cgactacgga gcctgaccac catgaagatc
     1141 accgccatcg aggccgttcc gctcgccatc ccgctccggc ccatgaaccc gccgtcgccc
     1201 tggacggggg gcacccgcaa acagatcttc gtgcgggtgc acaccgacga gggcgtcagc
     1261 gggctcggcg aggccttcgc ctacggggcg ccactcgcgg tggtgagcgt catcgaggag
     1321 agcctcgcgc ccctgatcgt cggccaggac ccgctgcgca tcgaggcggt ggtcgacctc
     1381 atgcatcggg gcacgatgat ctacgggcgg cgcgggctcg ggatgttcgc gatcagcggc
     1441 atcgagatcg cgctgtggga cctgctcggc aaggtgcgcg gcgcgccggt ctacgagctg
     1501 ctgggcgggg ccctgcgccc gcgcctgccc gcctacgcga gcctgctccg ctacgagacg
     1561 ccggacgcgg tggcgggcgc ctgccgccac ttcgtggcgc agggcttccg gatgctcaag
     1621 ctccaccaga ccgacgtggc ctccgtccgc gcggcccgcg aggcggtggg ccccgacgtg
     1681 gagctgatgc tcgacaccaa ctgcccgtgg acgcccttcg aggccatcgc gatggcgcgg
     1741 gcgctcgcgc cctatcgcct gttctggctg gaagagccgg tgtggccgcc ggaggactac
     1801 gcggggctcg cgcgggtggc cgccgccacc gagactccga tcgcgctggg cgagaacgag
     1861 tccaccgtgc acggcttcca cgagatcgtg cgccaggggg ccggcgacat cctgcagccg
     1921 agcatcacca aggtgggcgg catttccgag ttcaagaaga tcgccgcggt cgctcaggcc
     1981 gccaacctgc cgatcgcccc gcactccttc tatttcgggc ccgggttggc cgccaccctg
     2041 cacgtggccg ccacgttcgg cggcccgcta ccggtcgagt tcccgaccgg cgaccaggag
     2101 acgccgttcc tcacccggcc catccaggcc cgcgacggct ggctcgagct gccgagcggc
     2161 cccggcctgg gcgtggagat caacgaggaa gccctgcgcc gccacccctt cgtgaaaaac
     2221 gcagcccagc ccttcgtgct cagatagcgc gccgcgtgga tttcaccgag cgtgagctca
     2281 tggtcatcgc cgccgcgcga gagatccgcg acgacgacct ggtcttcgtc ggcatgcgcc
     2341 tgccgctgat cgccttcgcc tacgccaagc gcacccacgc gcccggggcg accgggctct
     2401 tcgagaacgg catcgtgcgc gaccagccgt cgccggagac gctgctcacg at
//
LOCUS       DATMCV010000944         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_317231, whole genome shotgun sequence.
ACCESSION   DATMCV010000944 DATMCV010000000
VERSION     DATMCV010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317231"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VNU03_21610"
     CDS             complement(<1..512)
                     /locus_tag="VNU03_21610"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWP77134.1"
                     /translation="MPREPSARGPLEEEGRPLFGRNPVLELLRSGSRRVEEIAIISEG
                     RGPALHDLLNLAKRQGVKISFRTRDQLTAIAGDPHHQGVVARVAGASYSTLGDLLTVP
                     AGRGEVPFFLALDQVQDPRNLGAVLRSAEVTGVHGVILPKHHAVGLTGAAAKSAMGAA
                     ELVAVARETNL"
     gene            complement(728..2158)
                     /gene="cysS"
                     /locus_tag="VNU03_21615"
     CDS             complement(728..2158)
                     /gene="cysS"
                     /locus_tag="VNU03_21615"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWP77135.1"
                     /translation="MALRAFNTLSRRKEDLAPGRRGEMRIYVCGVTVWDASHIGHGRS
                     AIVFDVIRRYIRHRGYAVKFVRNFTDIDDKIIRRANQEGVSAQEISERYIAEYRADLA
                     SLGVLPADVEPKATDHIPQMITLIERLIQQGVAYPVEGDVYFEVRRFPGYGKLSGKNL
                     EELQAGARVEVDERKRDPLDFALWKATKPGEPSWKSPWGDGRPGWHIECSAMAMQYLG
                     ETLDVHGGGEDLIFPHHENEIAQSEAATGKPFVRQWMHNGFVNLAAEKMSKSLGNTLT
                     IRDLVRRHDPEALRLYLLGTHYRHPLEFADERIGEAGRGLARLRSLKEEAERLAARGT
                     PAPGPDGGLFDQVAAQRARFEAAMDDDFNTPQALGVLFDLARLLHTAREQVAQGTVGA
                     GAFLMGVGELVMLSQVLGLLEGGGRRATAIDPQLKAKVESLVYLRQEARRQRDFAEAD
                     RLREELTRLGVVLEDTRDGTTWKLQS"
BASE COUNT          298 a          847 c          766 g          340 t
ORIGIN      
        1 aggttggtct cccgcgccac cgccaccagc tccgcggccc ccatggcgga cttggccgcc
       61 gccccggtga gcccgaccgc gtggtgcttc ggcaggatca ccccatggac ccccgtcacc
      121 tcggccgagc ggagcaccgc cccgagattg cgtggatcct gaacctggtc cagcgccagg
      181 aagaacggca cctcgccccg gccggccggg accgtcagca ggtcgcccaa cgtggagtag
      241 ctggcgcccg ccacccgggc gacgaccccc tggtggtgag ggtctccggc gatggcggtc
      301 agctggtccc gggtccggaa ggagatcttg accccctgcc gcttggccag gttcagaagg
      361 tcgtggagcg cgggtccccg cccctccgag atgatggcga tctcctcgac gcgacggcta
      421 cccgatcgga gcagctccag caccggattg cgcccgaaga ggggccggcc ctcctcctcg
      481 agcggcccgc gggccgatgg ctcccgcggc aattcctccc gcgacaatgg ctcccgagac
      541 ggcggctcgt gccgagccgg ctcccgtggg gcgggcggcc gcggcatctg aatccggggc
      601 gggctcgtcc gctgctccgg ccggggctct cgacggccct ccggtcggga tccaggccgg
      661 ggcgggtccc gccgcgacgg cccctggcgc ggaaatcctc cgcggggctc tcgtggacgc
      721 tcggggctca tgactggagc ttccaggtcg tcccgtcccg cgtatcctcc aggaccaccc
      781 cgagccgggt cagctcctcg cgcaaccgat cggcctcggc gaagtcgcgc tgccggcgcg
      841 cctcctgccg aagatagacg agcgactcga ccttggcctt gagctggggg tcgatggcgg
      901 tggcccggcg gcctccccct tcgagcaacc ccagcacctg gctcagcatc acgagctcgc
      961 cgacgcccat caggaacgcg cccgccccca cggtgccctg cgccacttgc tcccgggcgg
     1021 tgtggagcag acgtgccagg tcgaacagga cacccagcgc ctgcggggtg ttgaagtcgt
     1081 cgtccatggc ggcctcgaac cgggcccgct gcgccgccac ctggtcgaag agccccccgt
     1141 cgggcccggg cgcgggcgtc ccccgagcgg cgaggcgctc cgcctcctcc ttgagcgacc
     1201 gcagccgggc gagcccgcgt ccggcctcgc cgatccgctc gtcggcgaac tcgagtgggt
     1261 ggcgatagtg cgtgccgagc aggtagaggc ggagagcctc ggggtcgtgc cgccgcacca
     1321 ggtcccggat ggtcagcgtg ttgccgagcg acttcgacat cttctcggcg gccaggttca
     1381 cgaacccgtt gtgcatccac tggcgcacga agggcttgcc ggtggcggct tccgactgcg
     1441 cgatctcgtt ctcgtgatgc gggaagatca ggtcctcgcc cccgccgtgc acgtcgagcg
     1501 tctcgcccag gtactgcatc gccatcgccg agcactcgat gtgccagccc gggcgaccgt
     1561 cgccccacgg gctcttccac gagggctcgc ccggcttggt cgccttccag agcgcgaagt
     1621 cgagcgggtc gcgcttgcgc tcgtccacct cgacccgggc gcccgcctgc agctcctcca
     1681 ggttcttgcc cgagagcttg ccgtagcccg ggaaccgccg cacctcgaag tagacgtcgc
     1741 cctcgaccgg gtaggccacg ccctgctgga tcagtcgctc gatcagggtg atcatctgcg
     1801 ggatgtgatc ggtggccttg ggctcgacgt cggcgggcag cacgccgagc gacgccaggt
     1861 cggcccggta ctccgcgatg tagcgctcgg agatctcctg ggccgagacg ccctcctggt
     1921 tggcgcgccg gatgatcttg tcgtcgatgt cggtgaagtt ccgcacgaac ttcaccgcgt
     1981 agccacggtg ccggatgtag cggcggatca cgtcgaacac gatcgcgctc cgcccgtggc
     2041 cgatatggct cgcgtcccac acggtgacgc cgcagacgta gatccgcatc tcgccccggc
     2101 gaccgggcgc gagatcctcc ttccggcggc tcaaggtgtt gaaggcgcgc agggccatca
     2161 ggcggtctcc acgctgacca ccgccatcga ggcgatcccc tcaccgcgcc ccagcagccc
     2221 caggtgctcc gggctcttgg ccttgacgct c
//
LOCUS       DATMCV010000945         3117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_390161, whole genome shotgun sequence.
ACCESSION   DATMCV010000945 DATMCV010000000
VERSION     DATMCV010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390161"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..592)
                     /locus_tag="VNU03_21620"
     CDS             complement(140..592)
                     /locus_tag="VNU03_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWP77136.1"
                     /translation="MSGEPVVYFEDIELGEQYTSPGRTVTEADIVIFAGLSGDYNVLH
                     TDAEHMKASLFGERIAHGLLGLSIQQGLLDRVVPGQAVGPLAAVKWKFKGPIKIGDTI
                     HVEARVAAKKDGERPDAGLITVERRVFNQRGEVVQEGETDHVVGRRPA"
     gene            733..2151
                     /locus_tag="VNU03_21625"
     CDS             733..2151
                     /locus_tag="VNU03_21625"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP77137.1"
                     /translation="MQSRRQFILKGAKLVAVGAAAASAGPSVFVRGAGAADKQLKVLQ
                     WAHFVPAYDKWFDPWAKEWGAKRGVEVTVDHVGFADVVPRATAEVAAQSGHDVHMFIG
                     LASAFEEHVIDLKEVSGTLEKKYGKPVELATRSTYNPLTKKQFALSDMWVPDPGNYHK
                     KVWTDIGMPSGPATYEDLVKAAPEIKKVAPQMQIPIGVGLSQDIDSNMAVRNILWCHG
                     GSIQDKDGNLVLNSPETLKALEYAKRLYSVGMTQAVLSWNAASNNQAFNAQETAYILN
                     SISAYRTAQDNKLPVLDNYFFVPALKGPTGIQLASEHVMSGYVVWKFSKSQDIAKEFL
                     VALVDASRESMLGSKLYNFPSFYGAAAEPNTPMNKKAESGAAWIAKQCNADPFGSQPG
                     DKLSLLAKALPWSTNLGYPGFANPAEGEIFDTYVITDMFAKAATGALSPKDALTEATT
                     RCKEIFTKWRKKGMVAGGSKDR"
     gene            2223..>3117
                     /locus_tag="VNU03_21630"
     CDS             2223..>3117
                     /locus_tag="VNU03_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWP77138.1"
                     /translation="MASPDIALPRPRATPVETLSRRDLRLGKILLAPALTYIFLLVAI
                     PFLLAVFLSLTNSSAGSLDFSFVGLQNFRAVVSSPVFRRALWNTFVFTFVSQALVIVM
                     GNVLARALMKPFRGKLLVRFLILLPWAAPISLATLGWLWIFDSTFSVINWLLKVVGWL
                     GPGQWYYWLGDPVLGMIAIITVHVWRMLPFSTVILLAGLSSIPREVHEAADIDGAGPL
                     AKTFQITIPMMLPILTVAVLFGVVFTFTDMSVVYLLTRGGPYNSTHVLASLAFQDGVL
                     GGDVGRGAAVAIFLVPLLVVMA"
BASE COUNT          485 a         1067 c         1051 g          514 t
ORIGIN      
        1 cggctgagag tgagcgagcc gaagagcgcg gtgtgggtct cggtgagggt cacgcccggg
       61 gtctcgagcg ggacgttcat atgcacgtcc tcgaagtaga ggcgccgctc gagaggagcg
      121 ttcgccatgc cgcgatccct caggcgggcc ggcggccgac cacgtggtcg gtctcgcctt
      181 cctggaccac ctcgccgcgc tggttgaaca cgcgacgctc caccgtgatc aggcccgcgt
      241 ccggccgctc gccgtccttc ttggccgcca cgcgcgcctc gacgtggatg gtgtcgccga
      301 tcttgatcgg ccccttgaac ttccacttca ccgcggccag ggggccgacc gcctgcccgg
      361 gcaccacccg gtcgaggagg ccctgctgga tcgacaggcc gagcaggccg tgggcgatgc
      421 gctcgccgaa gagcgacgcc ttcatgtgct cggcgtcggt gtggagcacg ttgtagtcgc
      481 cgctcagccc cgcgaagatc acgatgtcgg cctcggtcac ggtgcggccg ggcgaggtgt
      541 actgctcgcc gagctcgata tcttcgaagt agacgaccgg ctcgccgctc acggggggtt
      601 aggatagcac gccggctcgc gccttcccgc gtcgggggat cgcggtgccg gtgaacccgt
      661 atggtacgct cgttgattgg aagcaggccg ggttcgccgg cctgaccgat gtccacacca
      721 acggaggtgg ccatgcagag tcgcaggcaa ttcatcctca agggtgccaa gctcgtggcg
      781 gtcggagcgg ccgccgcttc cgccgggccg agtgtcttcg tgcgcggagc gggcgcggcc
      841 gacaagcagc tcaaggtcct ccagtgggcg cacttcgtgc ccgcctacga caagtggttc
      901 gacccgtggg cgaaggagtg gggcgccaag cgaggggtcg aggtgacggt ggaccacgtg
      961 ggcttcgccg acgtggtgcc gcgcgcgacc gccgaggtgg cggcccagtc cggccacgac
     1021 gtccacatgt tcatcggcct ggcgagcgcc ttcgaggagc acgtgatcga cctcaaggag
     1081 gtgtcgggca cgctcgagaa gaagtacgga aagccggtcg agctggccac ccgcagcacc
     1141 tacaacccgc tcaccaagaa gcagttcgcg ctgtccgaca tgtgggtccc ggatccgggc
     1201 aactaccaca agaaggtgtg gaccgacatc ggcatgccga gcgggccggc gacctacgag
     1261 gatctggtga aggcggcgcc cgagatcaag aaggtggcgc cgcagatgca gatcccgatc
     1321 ggggtcgggc tctcgcagga catcgactcc aacatggcgg tgcgcaacat cctctggtgc
     1381 cacgggggct cgatccagga caaggacggc aacctggtgc tgaactcgcc cgagactctg
     1441 aaggcgctcg agtacgccaa gcggctctac tcggtgggca tgacccaggc ggtcctgtcg
     1501 tggaacgcgg cctcgaacaa ccaggcgttc aacgcccagg agaccgcgta catcctgaac
     1561 tcgatctcgg cctaccggac cgcgcaggac aacaagctac cggtgctcga caactacttc
     1621 ttcgtgcccg ccctcaaggg ccccaccggg atccagctcg cgtccgagca cgtgatgagc
     1681 ggctacgtgg tctggaagtt ctccaagagc caggacatcg ccaaggagtt cctggtggcc
     1741 ctggtggacg cctcgcggga gtcgatgctc gggagcaagc tctacaactt cccgtcgttc
     1801 tacggcgcgg ccgccgagcc caataccccg atgaacaaga aggcggagtc gggggcggcc
     1861 tggatcgcca agcagtgcaa cgccgatccg ttcggctcgc agccgggcga caagctctcg
     1921 ctgctcgcga aggccctgcc ctggtcgacc aacctgggct accccggctt cgcgaatccg
     1981 gcggagggcg agatcttcga cacctacgtg atcaccgaca tgttcgccaa ggcggccacc
     2041 ggggcgctct cgcccaagga cgccctgacc gaggcgacca cccgctgtaa ggagatcttc
     2101 accaagtggc ggaagaaggg aatggtcgcc ggcgggtcca aggaccgcta ggccactccc
     2161 gcgctgatcc gcgaccgggc cggcgggcgc ctcgtgcgcc ggccggcccg gtcgtgacca
     2221 cgatggccag ccccgacatc gctctccccc gcccgcgagc cacgcccgtc gagaccctct
     2281 cgcgccggga cctccggctc ggcaagatcc tgctcgctcc cgcgctgacc tacatcttcc
     2341 tactcgtcgc gatcccgttc ctgctcgcgg tcttcctgag cctcaccaac tcctccgcgg
     2401 gcagcctcga cttctcgttc gtgggcctgc agaacttccg ggccgtggtg tcgagcccgg
     2461 tgttccggcg cgccctgtgg aacaccttcg tcttcacgtt cgtctcgcag gcgctggtca
     2521 tcgtgatggg caacgtcctg gcccgggcgc tgatgaagcc gttccggggc aagctgctcg
     2581 tccggttcct gatcctgctc ccgtgggcgg ccccgatctc gctcgcgacc ctcggctggc
     2641 tgtggatctt cgactcgacc ttcagcgtga tcaactggct gctcaaggtg gtgggctggc
     2701 tcggccccgg ccagtggtac tactggctcg gcgacccggt gctcgggatg atcgcgatca
     2761 tcaccgtgca cgtgtggcgg atgctgccct tctcgaccgt gatcctgctc gccggcctca
     2821 gctcgatccc ccgggaggtg cacgaggccg ccgatatcga cggggccggg ccgctggcca
     2881 agacgttcca gatcacgatc ccgatgatgc tgccgatcct gaccgtcgcg gtgctcttcg
     2941 gggtggtgtt caccttcacc gacatgagcg tggtctacct gctcacccgc ggcgggccct
     3001 acaacagcac ccacgtgctc gcgagcctgg ccttccagga cggggtgctc gggggcgacg
     3061 tgggccgggg cgcggcggtg gcaatcttcc tggtgccgct gctcgtggtg atggcgg
//
LOCUS       DATMCV010000946         4284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_49355, whole genome shotgun sequence.
ACCESSION   DATMCV010000946 DATMCV010000000
VERSION     DATMCV010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49355"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VNU03_21635"
     CDS             <1..480
                     /locus_tag="VNU03_21635"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77139.1"
                     /translation="QALTRAAGAECWIESANRGHTAAVAFAQQQATTRGLPRFLTIPG
                     DVPCVTADEVATLCGALDETPGVVFVPSRSGLGTNAALLAPPGVMPLTFGEPSFANHL
                     AAARAAGLSPRVLELPGIGLDVDAPDDLPILLERGPHTRSARLLRGFPAGAPRAGAH"
     gene            482..1234
                     /gene="cofE"
                     /locus_tag="VNU03_21640"
     CDS             482..1234
                     /gene="cofE"
                     /locus_tag="VNU03_21640"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HWP77140.1"
                     /translation="MPARYEVIAVGGLPEIRRGDELAPLILEAARRQGTALQDRDLLV
                     VSQKIVSKTEGRVVRLADVAVSDEARSVADEIGRDPRLVQVILGESRRIVRKAKDVLI
                     VETRHGFVCANAGVDQSNVDADTACLLPEDSDRSAGALRAHLRTLTGHELAIIVADTF
                     GRPWREGLVNVAVGLAGLEPIVSYLGQRDPAGHVLQATILALADELAGAAEPVMGKLD
                     RVPVAIIRGLDWPPGDGSSRALLRDPARDLFR"
     gene            1255..1899
                     /gene="npdG"
                     /locus_tag="VNU03_21645"
     CDS             1255..1899
                     /gene="npdG"
                     /locus_tag="VNU03_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HWP77141.1"
                     /translation="MRIGIVGGTGKEGVGLGLRWAQAGHEVIIGSRDAERARAKAAEL
                     QGPGRRVDGKSNRDAAAAAEVVVLTVPATGLSATLPELREACRGKVVVSTVVPLTFGG
                     GRLFTPPPQGSAAEEAQEILGPEVRVVAAFHHIAAHELSAGDHAIACDLLLCGGDAEA
                     KKLVSDLGASMGLRPVDVGALTNAGPIEGITAVLATINRRYKLKNSGITITGLP"
     gene            1910..3001
                     /locus_tag="VNU03_21650"
     CDS             1910..3001
                     /locus_tag="VNU03_21650"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HWP77142.1"
                     /translation="MALDATDSPVTVSVHALTAFIVSVLEALKMSRRNATLTADLMVR
                     TDLRGVDSHGIGMLPRYVEWTRAGFVHPWADPTVARDDLATALVDGQKGLGHPASVMA
                     MELAITKAATYGVGVVAVRNSNHFGACANYSMMALERGMIGLAVTNSPFVAMVPTFAK
                     KPMMGTNPLSFAAPAARHTPFVLDMATTTVAVGKLTIASRWNKPIPEGWGLDAEGRPT
                     TSAKDVLASRLLSPLGGSRELGSHKGYGLGVMVDILSGVLSGGVYGDVLDRSGARGRK
                     ESNTGHCFAAIDVKRFRPLEDFTGAMDDMLQALTDTPRADGQERVYTAGEPEAETERQ
                     RRLHGIPVAPALVRQCNEFAADLGVTRLA"
     gene            complement(3005..3694)
                     /locus_tag="VNU03_21655"
     CDS             complement(3005..3694)
                     /locus_tag="VNU03_21655"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HWP77143.1"
                     /translation="MKLVLFDIDGTLITSRGAGRRAMRSAFERVFGTAGGIDTYDLRG
                     RTDTRIVHDVMGAEGWEPASVRERLDDFFEAYVGGLITEIGDGQGVVTLPGVATVVEQ
                     LARSSEAVLGLVTGNIEEGARIKLLPTGLWPTFRVGAYGSDHMDRRHLPSLAARRAHA
                     LLGREFPPADLVVIGDTPHDVDCARACGAVAVAVATGQYPREALLADGPDHFFEDLSD
                     TERVLRAILGG"
     gene            complement(3714..>4284)
                     /locus_tag="VNU03_21660"
     CDS             complement(3714..>4284)
                     /locus_tag="VNU03_21660"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWP77144.1"
                     /translation="SGNDLYGPPGSGALWVRPGVRVHPQVLGGGQEGGYRSGTENLPG
                     MVGFGVAAELMRVEGAHQEAARLAALRDRLLSGLLETIPDCRLTGSRSARLPHHLSLV
                     LRGVKADGVLLDLDLAGIAASSGSACASLTQTTSHVLRAIGCPAEEADGSLCFTLGRW
                     NTAADVDAVLERLPPIVARLRALAAPLHT"
BASE COUNT          584 a         1644 c         1512 g          544 t
ORIGIN      
        1 caggccctca cccgcgccgc gggcgccgag tgctggatcg agagcgccaa ccggggccac
       61 accgcggcgg tggcgttcgc ccagcagcag gccaccacgc gcggcctccc gcgcttcctc
      121 acgatccccg gcgacgtgcc ctgcgtcacc gccgatgagg tggccaccct ctgtggcgcg
      181 ctcgacgaga cacccggggt cgtcttcgtg ccgtcacggt ccggcctcgg caccaacgcc
      241 gcgcttctcg ccccgccggg cgtcatgccc ctcaccttcg gcgagccgtc gttcgccaat
      301 cacctggccg cggcccgcgc cgccgggctc tccccgcgcg tgctcgagct ccccgggatc
      361 gggctcgacg tcgacgcgcc ggacgatctc ccgatcctcc tcgagcgcgg cccccacacc
      421 cggagcgccc gcctgctccg gggcttcccc gccggcgcgc cgcgcgcggg cgcccactag
      481 tatgcccgcc cgctacgagg tgatcgcggt cggaggcctc ccggagatcc ggcgcggcga
      541 cgagctggcc ccgctcatcc tggaggccgc gcgccgccag ggcaccgcgc tccaggaccg
      601 cgacctcctg gtggtgagcc agaagatcgt gtccaagacc gaaggccgcg tcgtccggct
      661 cgccgacgtc gccgtgtcgg acgaggcccg ctcggtcgcc gacgagatcg gccgcgaccc
      721 gcgcctcgtc caggtcatcc tcggcgagag ccggcgcatc gtgcgaaagg ccaaggacgt
      781 gctgatcgtc gagacgcgcc acggcttcgt gtgcgcgaac gcgggcgtgg accagtcgaa
      841 cgtggacgcc gacaccgcct gcctcctgcc cgaggactcc gaccgctcgg cgggcgcgct
      901 ccgcgcccac ctgcgcacgc tcaccggtca cgagctcgcg atcatcgtcg cggacacctt
      961 cgggcggccg tggcgcgaag ggctcgtcaa cgtcgcggtc ggcctcgccg ggctcgagcc
     1021 gatcgtcagc tatctcgggc agcgggaccc ggccggccac gtgctccagg ccaccatcct
     1081 ggccctggcc gacgagctgg ccggggccgc cgagccggtc atgggcaagc tcgaccgggt
     1141 gccggttgcc atcatccgcg ggctcgactg gccgcccggc gacggctcga gccgcgccct
     1201 gctgcgcgat cccgcgcgcg acctgttccg ttaatcccga cccaaggaga gcacatgcgg
     1261 atcggcatcg tcggcggtac cggcaaggaa ggcgtggggc tcgggctcag gtgggcccag
     1321 gccgggcacg aggtgatcat cgggtcgcgc gacgcggagc gcgcccgcgc caaggccgcc
     1381 gagctccagg gacccggccg ccgggtcgac ggcaagtcca atcgcgacgc ggccgcggcc
     1441 gcggaggtcg tcgtgctcac ggtgccggcc accgggctct cggccacgct gcccgagctg
     1501 cgcgaggcct gtcgcggcaa ggtggtggtc agcaccgtgg tcccgctcac cttcggcggc
     1561 ggccggctct tcaccccgcc tccgcagggc tcggcggcgg aggaggccca ggagatcctc
     1621 ggcccggagg tccgggtcgt cgccgccttc catcacatcg cggcccacga gctctccgcc
     1681 ggcgaccacg ccatcgcgtg cgacctgctg ctctgcggcg gcgacgcgga ggccaagaag
     1741 ctcgtctccg acctgggcgc gtcgatgggg ctccggccgg tggacgtggg agccctcacc
     1801 aacgcggggc ccatcgaggg catcaccgcg gtgctcgcga ccatcaaccg gcgctacaag
     1861 ctcaagaact ccggcatcac gatcaccggg ctgccctgag gagcggatca tggccctgga
     1921 cgccaccgac agtcccgtca ccgtctccgt ccacgccctg accgccttca tcgtctcggt
     1981 gctcgaggcg ctcaagatgt cgcgccgcaa cgcgaccctg accgccgatc tcatggtccg
     2041 caccgacctg cgcggggtgg actcgcacgg catcggcatg ctgccccggt acgtcgagtg
     2101 gacccgcgcc gggttcgtgc atccgtgggc ggaccccacg gtggcccgcg acgacctcgc
     2161 caccgccctg gtcgacggcc agaagggcct cggccatccg gcctcggtca tggccatgga
     2221 gctcgccatc accaaggccg ccacctacgg ggtcggcgtc gtggcggtcc gcaactcgaa
     2281 ccacttcggg gcctgcgcga actactcgat gatggcgctc gagcgcggga tgatcggtct
     2341 cgcggtgacc aactctccgt tcgtggcgat ggtcccgacc ttcgcgaaga agcccatgat
     2401 ggggaccaac ccgctgagct tcgcggcccc cgccgcgcgc cacaccccgt tcgtcctcga
     2461 catggcgacc accaccgtcg cggtgggcaa gctcacgatc gcctctcgct ggaacaagcc
     2521 gatcccggag ggctggggcc tcgacgcgga ggggcgtccc accaccagcg cgaaggacgt
     2581 gctggcctcg cgcctgctct ccccgctcgg cggcagccgt gagctgggca gccacaaggg
     2641 ctacgggctc ggggtgatgg tggacatcct gtccggcgtg ctctcgggcg gcgtctacgg
     2701 cgacgtgctc gaccggagcg gcgcgcgggg ccgcaaggag tccaacaccg gtcactgctt
     2761 cgcggcgatc gacgtgaagc gcttccggcc gctcgaggac ttcacgggcg cgatggacga
     2821 catgttgcag gccctcaccg acaccccgcg cgccgacggc caggagcgcg tctacaccgc
     2881 gggcgagccc gaggccgaga ccgagcggca gcggcgcctc cacgggatcc cggtcgcccc
     2941 cgcgctggtc cggcagtgca acgagttcgc cgccgacctc ggagtgacgc gcctcgccta
     3001 gcgcctagcc accgaggatc gcgcgcagca cgcgctcggt gtcgctcagg tcctcgaaga
     3061 agtgatccgg gccgtccgcg agcagggcct cccggggata ctgtccggtg gcgaccgcca
     3121 ccgcgaccgc cccgcaggcc cgcgcgcaat cgacgtcgtg cggggtgtcc ccgatcacca
     3181 ccaggtcggc gggcgggaac tcgcggccga gcagggcgtg cgcgcggcgc gcggcaaggg
     3241 acggcagatg gcggcggtcc atgtgatccg agccgtaggc tcccacccgg aaggtcggcc
     3301 agaggccggt cggcaggagc ttgatgcggg cgccctcctc gatgttgccg gtcaccagcc
     3361 ccagcaccgc ctcggaggat cgggcgagct gctccaccac ggtggcgacg ccggggaggg
     3421 tgaccacccc ctgcccgtcg ccgatctcgg tgatcagccc acccacgtac gcctcgaaga
     3481 aatcgtcgag ccgctcgcgc acgctcgccg gctcccagcc ttccgcgccc atgacgtcgt
     3541 gcacgatgcg ggtgtcggtg cgcccgcgga gatcgtaggt gtcgatcccg cccgccgtcc
     3601 cgaagacccg ctcgaaggcc gatcgcatcg cgcgacgccc agccccccgc gaggtgatca
     3661 gggtgccgtc gatgtcgaac aggacgagct tcacgcgcgg gtcggccggg cggctaggtg
     3721 tgcagcggag cggcgagcgc gcgcaggcgc gccacgatgg gcggcaggcg ctccagcacc
     3781 gcgtccacgt cggccgcggt gttccagcga ccgagggtga agcagaggga gccgtccgcc
     3841 tcctcggcgg ggcagccgat ggcgcgcagc acgtgcgacg tcgtctgggt gagcgacgcg
     3901 caggccgagc ccgaggaggc cgcgatgccg gccaggtcca ggtcgagcag gacgccgtcg
     3961 gccttcaccc cgcggaggac caggctcagg tggtgaggga gccgcgcgct tcgtgagccg
     4021 gtgagccggc aatcgggaat ggtctcgagc agcccgctca gcagccggtc gcgcagggcc
     4081 gcgagccggg cggcctcctg gtgcgcgccc tccacccgca tcagctccgc ggccaccccg
     4141 aagccgacca tgccgggcag gttctcggtg ccggagcggt agccgccctc ctggcctcca
     4201 cccagcacct gagggtggac gcgcacgccg ggacgcaccc acagcgcccc gctcccgggc
     4261 gggccgtaca ggtcgttccc ggag
//
LOCUS       DATMCV010000947         4364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_252495, whole genome shotgun sequence.
ACCESSION   DATMCV010000947 DATMCV010000000
VERSION     DATMCV010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252495"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VNU03_21665"
     CDS             <1..194
                     /locus_tag="VNU03_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77145.1"
                     /translation="TAAYLPSDSPWARRALVGAGQAFAALKQNDAAVIVYRKLLAMNG
                     VEADLAEAARRGLRALGAS"
     gene            complement(287..835)
                     /locus_tag="VNU03_21670"
     CDS             complement(287..835)
                     /locus_tag="VNU03_21670"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HWP77146.1"
                     /translation="MDDLLAAALAQVTVQDEAGAAVPFKGLWTERTVVLAFVRHLGCI
                     FCRQQVAGLTKRLPEIERRGATLVVVAPAKPEHIGPFRQATGYAGALYVDRSGRAFRT
                     AGLVRGWGSTYHLRAVLKGVVALAKGFRQAGRQGDVVQQGGTFVLGPGDRVHYEWRDR
                     FAGDNANLDAVVGAIPSTAAVK"
     gene            884..2701
                     /locus_tag="VNU03_21675"
     CDS             884..2701
                     /locus_tag="VNU03_21675"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HWP77147.1"
                     /translation="MRGYGGRILFVDVTTGASRVEPVSETLARTLLGGNGFAARLLLD
                     HVPPGVDAYDPANAVVFAVGPVTDTTVPGNSRACVAGKSPLTGLFFDSTFGGRFPATM
                     KRTGFDAIVITGCAAEPVYVKVTEAGAELRPARSLWGQTTRDTVQAIHAVEGGDADVM
                     AIGPGGERLVRFSAMGSYWKNREAFAGRGGLASVLGSKRVKAVVVAGARKTEVADPAA
                     LKALLEQTREPLKTGTKALSTYGTPFLVKPINTLGALGSYNLRQEVFADAVAISGEEM
                     HAHFHDKDTTCLKCPVACGKQYEVREGELAGLKAKMPEYETIFAFGSMLGNAHAGSLA
                     RANDLCDLLGLDTISMGVTLAFVCEAVERGWISERELGLPFGWGDWRGMLRLVEMTAM
                     REGFGDRLAEGAWRLAESVHPEGTRLVYAVKRLELPAHSARALKGMSIGYATATRGGS
                     HHDTRPTPQYAQNFDRRSPDGKPEFAIRSQHFTAVDDSLVLCRFTSERGFGLYVDEPY
                     ARMVRAITGWDMPVEELERVGERVINLERLFNVREGVRRGDDALPWRVMHEPIPDGPS
                     AGAYCPPAELDGMLDRYYALRGWDADGVPTPGRLATLDL"
     gene            2718..>4364
                     /locus_tag="VNU03_21680"
     CDS             2718..>4364
                     /locus_tag="VNU03_21680"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HWP77148.1"
                     /translation="MPVTRFDVTLRRPLAGFPGYEELKGRLHFAIDPLHESNRRITDV
                     ELAPRDAAGPVTWDSDVSILLPVDRAECTGRVLLDVVNRGNTVAVPNFNRATRPAFTA
                     GSDPNPPIDVGDGFLMKRGWVVISCGWQGDLPRVPGLIGMRTPEARDAQGRPLRGRIY
                     TQVQSTGPVADFLLSDRGHVPYPAADLAEPDALLTVRDQPDGPATTIARERWRFARSA
                     GGRIVPDAGHIHLEGGFEKGRLYQVTYTAEGAPVQGLAMAALREAVAWLKHGGAAEGN
                     PAPGRLRWAYAYGRSQTGRLLRTLVYEDLNLDEQGREALDGVIANVPGGMRGEFNQRF
                     GQHSKDRNNMMAHLFPFTDQPAADPETGAKDALHARLDARGSRLRVFYTNTSAEYHRG
                     DASLIHTDPDGQRDAVLGPYTRVYHFAGTEHGLGIWPPTDTQTAPADPTGPVERSQNL
                     RGVVDYSPLLRACLMHLDRWVVEGIEPPPSRHPRLADGTAVPPDRLAPAFARIPAARY
                     PRHNPRPRRLDWATLPPTPGRAFGSLVSAVNGDGNEIGGIV"
BASE COUNT          601 a         1654 c         1539 g          570 t
ORIGIN      
        1 tgaccgcggc ctatctcccg agcgattcac cctgggcgcg ccgcgcgctc gtgggagccg
       61 gccaggcctt cgccgcgctc aagcagaacg acgccgcggt gatcgtgtac cggaagctgc
      121 tcgcgatgaa cggggtcgag gccgacctgg ccgaggccgc gcgccgcggc ctccgcgccc
      181 tcggcgcctc ctagcctctc cctctcccct caggggagag ggcagggtga ggggcgggcc
      241 ggtgaggggc gcgccggtga ggggcggccc ggtgaggggt gttgcctcac ttcaccgccg
      301 cggtagacgg aatcgccccc accaccgcgt cgagattcgc gttgtccccc gcgaagcggt
      361 cgcgccactc gtagtgcacc cggtcgcccg gcccgagcac gaacgttccg ccctgctgca
      421 caacatcccc ctggcgcccg gcctgacgga agcccttcgc gagcgccacc acgcccttca
      481 gcaccgcgcg gaggtgatag gtgctgcccc acccgcgcac gagcccggcg gtccggaacg
      541 cgcgccccga ccggtcgacg tagagcgcgc ccgcgtagcc ggtggcctgc cggaagggcc
      601 cgatgtgctc gggcttcgcg ggcgccacca ccacgagcgt agccccgcgc cgctcgatct
      661 cgggcaggcg cttcgtcagc ccggccacct gctggcggca gaagatgcag cccaggtggc
      721 ggacgaaggc cagcaccacg gtgcgctcgg tccagagccc cttgaagggg accgccgcgc
      781 cggcctcgtc ctgcacggtg acctgggcca gcgcggcggc gagcagatcg tccatggcat
      841 gtcattgtaa ccggtccaca ttgtcactaa aatgggccgc gccatgcggg gatacggcgg
      901 ccggatcctc ttcgtcgacg tcaccaccgg ggcgagccgc gtcgagccgg tgtcagagac
      961 gctggcccgg acgttgctcg gcggcaacgg cttcgcggcg cggctgctcc tcgatcacgt
     1021 gccgcccggc gtcgacgcgt acgacccggc caacgcggtg gtcttcgcgg tgggcccggt
     1081 caccgacacc acggtgccgg gcaacagccg cgcctgcgtg gccggcaagt cgcccctgac
     1141 cggcctcttc ttcgactcga ccttcggcgg gcgcttcccg gccaccatga agcgcaccgg
     1201 cttcgacgcc atcgtgatca ccgggtgcgc ggccgagccg gtctacgtca aggtcaccga
     1261 ggcgggcgcg gagctgcgcc ccgcgcgctc gctgtggggc cagaccaccc gcgacacggt
     1321 gcaggcgatc cacgcggtgg agggcggcga cgccgacgtg atggccatcg ggccgggcgg
     1381 cgagcgcctg gtgcgcttct cggcgatggg cagctactgg aagaaccgcg aggcgttcgc
     1441 ggggcgcggc ggcctggcct cggtgctggg cagcaagcgg gtcaaggcgg tggtggtggc
     1501 gggtgcgcgc aagaccgagg tggccgaccc cgccgcgctc aaggctctgc tcgagcagac
     1561 gcgcgagccg ctcaagaccg ggaccaaggc gctctccacc tacggcaccc ccttcttggt
     1621 caagccgatc aacaccctgg gcgcgctcgg ctcctacaac ctgcggcagg aggtcttcgc
     1681 ggacgcggtg gcgatctccg gcgaggagat gcacgcccac ttccacgaca aggacaccac
     1741 ctgcctcaag tgcccggtgg cgtgcggcaa gcagtacgaa gtccgcgagg gcgagctggc
     1801 cggcctcaag gccaagatgc cggagtacga gacgatcttc gccttcggct ccatgctcgg
     1861 caacgcgcac gcgggctcgc tcgcccgggc caacgatctc tgcgacctgc tcgggctcga
     1921 caccatcagc atgggggtga cgctcgcctt cgtctgcgag gcggtcgagc gtgggtggat
     1981 ctccgagcgc gagctcgggc tgccgttcgg ctggggcgac tggcgcggga tgctgcgcct
     2041 ggtcgagatg accgcgatgc gtgaaggctt cggcgatcgg ctggccgagg gcgcgtggcg
     2101 gctcgcggag tcggtgcacc cggagggcac gcgcctggtc tacgcggtga agcggctcga
     2161 gctgcccgcc cactccgcgc gggcgctcaa gggcatgtcg atcggctacg ccaccgccac
     2221 ccgcggcggc agccaccacg acacgcggcc caccccgcag tacgcccaga acttcgaccg
     2281 gcgcagcccc gacggcaagc ccgagttcgc gatccggagc cagcacttca ccgcggtcga
     2341 cgactcgctc gtgctctgcc gcttcacctc cgagcgcggc ttcggcctct acgtcgacga
     2401 gccctacgcc cggatggtcc gggcgatcac cggctgggac atgccggtcg aggagctgga
     2461 gcgcgtcggc gagcgcgtga tcaacctgga gcgcctcttc aacgtgcgcg agggcgtgcg
     2521 ccgcggcgac gacgcgctgc cctggcgggt catgcacgag cccatccccg acggtccctc
     2581 ggccggcgcc tactgtcccc cggccgagct ggacgggatg ctcgaccgct actacgcgct
     2641 ccggggctgg gacgccgacg gggtgccgac gcccgggcgt ctcgccactc tcgacctcta
     2701 gccctccaga ggaccccatg ccggtcaccc gcttcgacgt cacgctgcgc cggcccctgg
     2761 ccggcttccc gggctacgag gagctgaagg ggcgcctgca cttcgccatc gatccgctcc
     2821 acgagtcgaa ccggcgcatc accgacgtgg agctggcccc gcgggacgcg gccgggccgg
     2881 tcacgtggga ctcggacgtg tccatcctgc tgccggtgga ccgcgccgag tgcacagggc
     2941 gtgtcctgct ggacgtcgtc aaccgcggca acacggtggc ggtacccaac ttcaatcgcg
     3001 cgacccggcc cgccttcacg gccggctccg acccgaaccc gccgatcgac gtcggtgacg
     3061 gcttcctgat gaagcgcggc tgggtggtca tctcgtgcgg atggcagggg gacctgccgc
     3121 gtgtgcccgg cctcatcggc atgcgcacgc cggaggcgcg ggacgcacag ggccgtccgc
     3181 tgcgcgggcg gatctacacc caggtgcagt ccaccggacc ggtggccgat ttcctgctct
     3241 ccgaccgcgg ccacgtgccg tatccggccg cggacctcgc ggagccggac gcgctcctca
     3301 ccgtgcgcga tcagcccgac ggcccggcca ccacgatcgc gcgcgagcgc tggcgcttcg
     3361 cccggagcgc gggcgggcgc atcgtcccgg acgcgggcca catccatctc gagggcggct
     3421 tcgagaaggg ccggctctac caggtgacgt acaccgcgga gggcgcgccg gtgcaagggc
     3481 tcgcgatggc cgcgctccgc gaggcggtcg cctggctcaa gcacggcggc gcggccgagg
     3541 gcaacccggc gcccggccgc ctgcggtggg cctacgccta cggccgctcg cagaccgggc
     3601 ggctgctgcg cacgctggtc tacgaggacc tcaacctgga cgagcagggg cgcgaggccc
     3661 tcgacggcgt catcgccaac gtgcccggcg gcatgcgggg cgagttcaac cagcgcttcg
     3721 gccagcactc gaaggaccgc aacaacatga tggcgcacct cttcccgttc accgaccagc
     3781 ccgcggcgga tccggagacc ggcgccaagg acgcgctgca cgcgcgcctc gacgcccgcg
     3841 gcagccggct gcgcgtcttc tacaccaaca cctcggccga gtaccaccgc ggcgacgcct
     3901 cgctgatcca caccgatccc gacggccagc gcgacgccgt gctcggcccg tacacgcgcg
     3961 tctatcactt cgcggggacc gagcacggcc tcggcatctg gccgccgacc gatacccaga
     4021 ccgcgcccgc cgatccgacc ggcccggtgg agcgctcgca gaacctccgc ggcgtggtgg
     4081 actactcgcc gctgctgcgc gcctgcctga tgcacctcga ccgctgggtg gtcgagggga
     4141 tcgagccgcc gccgagccgc cacccgcgcc tggccgacgg caccgcggtg ccgcccgatc
     4201 gcctcgcccc ggctttcgcg cgcattccgg ccgcgcgcta cccgcgccac aacccgcggc
     4261 cgcgacggct cgactgggcg acgctgccgc ccaccccggg ccgcgccttc ggctcgctcg
     4321 tctccgcggt gaatggcgac ggcaacgaga tcggcggcat cgtg
//
LOCUS       DATMCV010000948         2397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_49607, whole genome shotgun sequence.
ACCESSION   DATMCV010000948 DATMCV010000000
VERSION     DATMCV010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49607"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..1258)
                     /locus_tag="VNU03_21685"
     CDS             complement(275..1258)
                     /locus_tag="VNU03_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP77149.1"
                     /translation="MLAIAVQAHGGLDQLKLHTDWPEPVAGPGQAVVEVKACGLNYLD
                     IFVLHGMPGLPVEMPRIPGGDIAGIVHSVGAGVSREWLGKRVLVDPHIKHGGALGEHG
                     NGGLCERIAVDGDNLIHLPDAVTFEQAASLPIAYGTAYRMMITRGRLQPGELVLILGA
                     SGGVGTGCVQIAKNLKARVIACASSASKLRRLQELGADHVIDYTKEDFSKRAWEISGK
                     KGVDVLVNYTGGDTWVPSLRTLARHGRLLTCGATAGFDPKTDIRYIWRREVNIIGANG
                     WTRQDLEALLVEVERGSIKPIIHGVYPLAESREAMRVMEDREVFGKIIVVP"
     gene            complement(1314..>2397)
                     /locus_tag="VNU03_21690"
     CDS             complement(1314..>2397)
                     /locus_tag="VNU03_21690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77150.1"
                     /translation="TALGYSLSWLGFVFFAPIAGWLSDRWGTRTVVTCGGVILGVALA
                     LTGQMASLAEYYLYFGLLGAAGIAGMLIPSTTIVTRWFVRSRGTAMGVLSTGPGSAVV
                     FYPLNAWLIASLGWRTALAVFGCIVAVVTVLLTLLYREPPAVPAGRPADGGDGAAQSP
                     LAPAEMWTLRRALGSGRLWAAFTMTALGVIGFQIMATHQVAHAVDRGVPRDTVVWLFA
                     LAAGCMMAGNVLGGWLSDRVGRGWVFVLGTIGAILGILCFTLMRGPQDHVLLLLYALS
                     GFGFGMRIAQLSTIPADVFAGPHLGAILGVVQAGGGLGGAIGPYLAGWLFDVTGSYRL
                     AFLAACVAVAGSAVAAWFAARATASD"
BASE COUNT          391 a          878 c          803 g          325 t
ORIGIN      
        1 cccctcgcca ccagtccggc ggcgaccgcg tcgcagttgg cgtccagctc ggcgtaggtg
       61 acctcgcggg gggtagctgg acgggagagg tcgattatcg ctactcggtc gttcttaaaa
      121 ctcccctcac cctgccctct cccctgaggg gagagggtgg agttcggcgt ggggtttgtg
      181 gtccctctcc cctcagggga gagggcaggg tgaggggtgg tccctccggt ggtccccccc
      241 gcgctcctcc ccacgagcac cccaacattg atcctcacgg aacgacgatg atcttgccga
      301 agacctcgcg gtcttccatg acccgcatgg cctcgcgcga ttcggccaag gggtacacgc
      361 cgtggatgat cggcttgatg ctcccgcgct ccacctcgac cagcagcgcc tccagatcct
      421 gccgcgtcca gccattggcc ccgatgatgt tgacctcgcg ccgccagatg taccggatgt
      481 cggtcttcgg atcgaagccc gcggtggccc cacaggtcag cagacgcccg tgccgggcca
      541 gagtccgcag gctcggcacc cacgtgtcgc cgcccgtgta gttcacgagc acgtccacgc
      601 ccttcttccc ggagatctcc cacgcccgct tggagaagtc ctccttcgtg tagtcgatga
      661 cgtggtccgc gcccagctcc tggagccgcc gcagcttgct ggcggaggac gcgcacgcga
      721 tcacccgcgc cttcaggttc ttggcgatct gcacgcagcc ggtgccgacg ccgccgctgg
      781 cacccaggat cagcaccagc tcgccgggct gcaggcggcc gcgcgtgatc atcatgcgat
      841 aggcggtgcc gtaggcgatg ggcagcgagg cggcctgctc gaaggtgacc gcgtccggca
      901 gatggatcag gttgtcgccg tccaccgcga tgcgctcgca caggcctcca ttgccgtgct
      961 cgcccagcgc gccgccgtgc ttgatgtgcg ggtccaccag cacccgcttg cccagccact
     1021 cgcgcgagac gccggcaccc accgagtgga cgatccccgc gatgtcaccg cccgggatgc
     1081 gcggcatctc caccggcagc ccgggcatgc cgtgcagcac gaagatgtcg aggtagttga
     1141 ggccgcaggc ctttacttcc accaccgcct ggccgggccc ggccaccggc tcgggccagt
     1201 ccgtgtgcag tttgagctgg tcgaggcccc cgtgggcctg gacggcgatc gcgagcatgg
     1261 cccttgatgt accattctcg gccgacacac cacaaccggg acgggactcc tcctcaatcg
     1321 ctcgcggtgg cccgtgccgc gaaccacgcg gcgacggccg agcccgcgac cgcgacgcag
     1381 gcggccagga acgccagccg gtagctgccg gtgacgtcga agagccatcc ggccagatag
     1441 gggccgatgg cgccgcccag gccgcccccc gcctggacca ccccgaggat cgcgcccagg
     1501 tgcggcccgg cgaagacgtc ggcgggaatc gtggagagct gcgcgatccg catgccgaag
     1561 ccgaatccgg acagggcgta gagaaggagg aggacatggt cctgggggcc gcgcatcagg
     1621 gtgaaacaca ggatcccgag aatcgccccg atcgtgccga gcacgaacac ccatccgcgc
     1681 ccgacccgat ccgagagcca gccgccgagc acgttgccgg ccatcatgca cccggccgca
     1741 agcgcgaaga gccacaccac cgtgtcacgc ggcacgccac ggtccaccgc gtgagcgacc
     1801 tggtgggtgg ccatgatctg gaagccgatg accccgagcg cggtcatggt gaaggcggcc
     1861 cagagccggc cgctccccag cgcgcggcga agcgtccaca tctcagcggg agcgagcggg
     1921 ctctgggcgg cgccgtcgcc cccgtcggcc gggcgacccg cggggacggc cggcggctcg
     1981 cggtagagca gcgtgagcaa gacggtcacg accgccacga tgcacccgaa gaccgcgagc
     2041 gccgtccgcc agcccaggct cgcgatgagc cacgcgttca gcgggtagaa gacgaccgcg
     2101 cttcccggtc cggtgctcag cacccccatc gcggttccgc gcgaccgcac gaaccaccgc
     2161 gtcacgatcg tcgtcgaagg gatgagcatc ccggcgatgc ccgcggcgcc gaggaggccg
     2221 aagtaaaggt agtactccgc gagggacgcc atctggccag tgagcgcgag ggccaccccg
     2281 aggatcacgc cgccgcatgt cacgaccgtc cgcgttcccc agcggtcgga gagccatccc
     2341 gcgatcggcg cgaagaagac gaagcccagc cagctcagcg agtacccgag cgcggtc
//
LOCUS       DATMCV010000949         5508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_118209, whole genome shotgun sequence.
ACCESSION   DATMCV010000949 DATMCV010000000
VERSION     DATMCV010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118209"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..808
                     /locus_tag="VNU03_21695"
     CDS             77..808
                     /locus_tag="VNU03_21695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWP77151.1"
                     /translation="MPKTAGIILVGNEILSGKITDANAAYLCRELRALGVEVRRITVI
                     PDEVDLIAAEVRAFSAAYDVVFTSGGVGPTHDDVTIEGVARALDVPVIREPRLVQVLE
                     GYYKGRLNEARLKMAEVPQGAELTADGQLVFPAVVVRNIYILPGVPEIFRQKFDALKE
                     RFRDAPFHLCCVYVSMGEGTLADHLNELLAGHPDLMLGSYPEFSNPEYKVKVTLESKD
                     RGYLDAAVGSFLGRLPKEAIVRVER"
     gene            951..2561
                     /locus_tag="VNU03_21700"
     CDS             951..2561
                     /locus_tag="VNU03_21700"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWP77152.1"
                     /translation="MLHGAGWITSHLDGVPYFDKPPLLYWLMAGAFAWLGPTESAARL
                     VSGLSAVAVAVITVLIGTRLASARVGLMAGLMVAANLEMFLFGRMVKPDLLFVACILL
                     GLYGFVLAYLGKGRWPLLLAYGSLGLAVLAKDLLGALGPLAIVALFLALTGEGRAWRQ
                     WIPPRAVLLLLAVAVPWYVAVELQHRGFLWYTIVDNHVLNVTRQRVFPDEDVSLSTLE
                     FLAVTAAGFFPWVLALPLATWRALARPWRTPEARLWALLGIWVVAILGIFALSPFKLP
                     HYGLPAFPAMALLVARLWDDAIERPAGAPRSFTLLVPSLLTVAALAVASLLVWRGVLG
                     VSTGAVALADVSARNMAAQGQPVATDFIAQFRPLFGSISAIFGLVAAAVAMATWRRLP
                     RLGVGAMLAGMLAFLPLSVEGLAIFAKSRSVRVMTDALLLRAAPGDVVAHEGALENSA
                     SALLRLDRRVQIVNGQQSNLAFGATFPEARGTFWDGEALARAWRADRRVFLLTALTPA
                     KSVVRELPPGEVHLLLEGGGRRLYSNRP"
     gene            2600..3286
                     /locus_tag="VNU03_21705"
     CDS             2600..3286
                     /locus_tag="VNU03_21705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP77153.1"
                     /translation="MSEPVYRLKDDPHSSHSVILARLGEGRGRKALDVGAADGFLAER
                     LTGQGWNVTALERDPALAARAHGRCKEVVVADLEAAPPLLDGPFDAIVYGDVLEHLSD
                     PRAALVALDRTLAPGGIVIVSVPNVAHLWVRLSLLAGRFDYADRGILDRTHLRFFTRR
                     TLVAFLHSAGLAVAELRVTPVPLPLVVPPRWHGAGLRGLHDLSARVARLWPGGLAYQF
                     VAVCRVEGLP"
     gene            3286..4083
                     /locus_tag="VNU03_21710"
     CDS             3286..4083
                     /locus_tag="VNU03_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012472656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWP77154.1"
                     /translation="MERPAQVERPRVAPKVVVVMPAYNAGRTLRMTYEELPKDAVNLV
                     ILVDDGSTDTTLEVARDLGLEIFVHDRNYGYGANQKTCYTEALKAGADIVVMVHPDYQ
                     YDPTLVPQIIAPIVRGDADLVLGSRLASGGSAVQQGMPWWKFLANRFLTEVENVAFGL
                     RLSEYHTGYRAFSRAVLEDVNFRMNSDGFVFDQEIVAQVVSTGFRIAEIPVPVRYFPE
                     ASSASFAASCVYGLKILWVVARYLLHRSGIKRSRRLASIVHRYHRYR"
     gene            4080..>5508
                     /locus_tag="VNU03_21715"
     CDS             4080..>5508
                     /locus_tag="VNU03_21715"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HWP77155.1"
                     /translation="MIARLLAVAAAVAAAGLGLALVLWTGRREHWPWRPEHFFLLLLG
                     LLALRVLIAPRPIPALRPRRVVAVGVAVYALLFSFVTVTRHFTFATHALDLGYYVQLG
                     WNLARGAGPYVSLPEMHAWGDHLSPIMYAFVPAFWIAPGPVVLLIAQSAALALGGAAV
                     FGLARARLGDERPAAAFALLYLANPSLQGINVRDFHTAALAIPLLLAAAWAVEAGRPW
                     WALAPAALTLLCREDAALPVIGLGAWMALARRRWAPGAIAAVGALAVLAIDVRFIVPA
                     YRGEPYSHLGRYAQLGDSLGAIVTTIVLHPLHTLASLITADRMLYLAAMLAPLGLLPL
                     LGGWDLVGVLPGLAQNLLSVDPVLYNYRAQYQAFVLPFLVLAAIGGYARLERRRPGHW
                     PVAVLVAAMVVSLALDSRTANNLAVARFWPTPAQMAAYRVLAAVPPEVAVTAQDPYVP
                     HLSLRPRVFVFPVGIDKSDYVLVDLE"
BASE COUNT          621 a         2079 c         1898 g          910 t
ORIGIN      
        1 aaccgagcaa tggtctcccg cgccccgaag ttcgttggac gcgccgcatt ctagcatcgt
       61 aaaatttccg tcgaccatgc cgaagacggc cgggatcatc ctcgtcggca acgagatcct
      121 ctcgggcaag atcaccgacg ccaacgccgc ctacctctgc cgcgagctgc gcgcgctggg
      181 cgtcgaggtg cggcgcatca cggtcatccc cgacgaggtg gacctgatcg cggcggaggt
      241 gcgcgcgttc agcgccgcct acgacgtggt gttcacctcc ggcggggtgg gcccgaccca
      301 cgacgacgtg acgatcgagg gcgtcgcgcg cgccctcgac gtgccggtca tccgggagcc
      361 gcgactggtc caggtgctgg agggctacta caagggccgg ctcaacgagg cgcggctcaa
      421 gatggcggag gtgccgcagg gcgccgagct caccgccgac ggccagctcg tcttccccgc
      481 ggtggtggtg cggaacatct acatcctgcc cggggtgccc gagatcttcc gccagaagtt
      541 cgacgcgctc aaggagcgct tccgcgacgc gcccttccac ctctgctgcg tctacgtgag
      601 catgggggag ggcaccctgg ccgatcacct gaacgagctg ctggccggcc atccggacct
      661 gatgctcggc tcgtacccgg agttctccaa ccccgagtac aaggtgaagg tcacgctcga
      721 gtcgaaggac cgcggctacc tcgacgccgc ggtcggcagc ttcctgggcc gcctcccgaa
      781 ggaggcgatc gtccgggtcg agcggtgagc cgccgccgcg gcggcgggcg gctccgctga
      841 ccccgccgct ggcggtcgcc atcgtcgcgg cggccctctt tctcggccgc ctcggggccg
      901 cccccttcct cgatccgccc gagggtatcc acgcccagat cgcgtgggag atgctgcacg
      961 gggcgggctg gatcacgtcg catctcgacg gggtcccgta cttcgacaag cctccgctcc
     1021 tctactggct gatggcgggc gccttcgcct ggctcggccc gaccgagagc gcggcgcgtc
     1081 tcgtctcggg cctctccgcg gtggcggtgg cggtgatcac cgttctcatc ggcacgcgcc
     1141 tcgcctccgc ccgcgtgggg ctgatggcgg ggctgatggt cgcggccaac ctcgagatgt
     1201 tcctcttcgg ccgcatggtc aagccggacc tgctcttcgt cgcctgcatc ctgctcggcc
     1261 tctacggctt cgtgctcgcg tacctgggga agggccggtg gccgctcctg ctcgcgtacg
     1321 gcagcctcgg cctggcggtg ctcgcgaagg atctcctggg cgcgctcggc ccgctcgcca
     1381 tcgtggcgct cttcctggcc ctcaccggcg agggacgggc gtggcgacag tggatcccgc
     1441 cgcgcgcggt cctgctcctg ctcgcggtcg cggtcccgtg gtacgtggcg gtggagctcc
     1501 agcaccgcgg cttcctctgg tacacgatcg tcgacaacca cgtgctcaac gtcacgcgcc
     1561 agcgcgtgtt ccccgacgag gacgtatcgc tctcgacgct cgagttcctc gcggtgaccg
     1621 cggccggctt cttcccgtgg gtgctcgccc tgccgctcgc gacgtggcgc gcgctcgcgc
     1681 ggccgtggcg cacgcccgag gcgcggctct gggccctgct cgggatctgg gtggtggcga
     1741 tcctgggtat cttcgcgctg tcgccgttca agctgccgca ctacggcctg cccgcgttcc
     1801 cggccatggc cctgctggtg gcccggctct gggacgacgc gatcgagcgg cccgcgggcg
     1861 cgccccgctc cttcaccctg ctcgtcccgt ccctgctgac ggtggccgcg ctcgcggtcg
     1921 cgtcgctgct ggtgtggcgc ggggtgctcg gggtctcgac gggggcggtc gcgctcgccg
     1981 acgtctccgc ccgcaacatg gcggcccagg gccagccggt cgcgaccgac ttcatcgccc
     2041 agttccgtcc gctgttcggg agcatcagcg cgatcttcgg gctggtggcg gccgcggtcg
     2101 cgatggccac ctggcggcgc ctgccgcggc tcggggtggg ggcgatgctc gcgggcatgc
     2161 tcgccttcct tccgctctcg gtcgaggggc tcgcgatctt cgccaagtcc cgctcggtgc
     2221 gggtgatgac cgacgcgctc ctgctgcggg ccgccccggg ggacgtggtg gcccacgagg
     2281 gagcgctcga gaacagcgcg tccgccctcc tgcgcctgga ccggcgggtg cagatcgtca
     2341 acggccagca atcgaacctg gccttcggcg ccacgttccc cgaggcccgg ggcaccttct
     2401 gggacggcga ggcgctggcc cgcgcctggc gcgccgaccg ccgggtgttc ctcctgaccg
     2461 cgctgacccc ggccaagagc gtggtgcgcg agctgccgcc cggggaggtg cacctgctgc
     2521 tcgaaggcgg cggccgccgg ctctattcga atcggccctg agggacccgg atgatatcct
     2581 gattcgtagg cggaaaagca tgagcgaacc ggtctaccgg ctcaaggatg acccgcactc
     2641 gagccattcg gtcattctcg cccgactggg ggagggacgc ggccggaagg cgctcgacgt
     2701 gggcgcggcg gacgggttcc tcgccgagcg gctcaccggg cagggctgga acgtcaccgc
     2761 gctcgagcgc gatcccgcgc tggccgcccg cgcccacggg cgctgcaagg aggtcgtggt
     2821 cgccgatctc gaggcggccc cgcctctgct cgacggcccg ttcgacgcca tcgtctacgg
     2881 cgacgtgctc gagcacctga gcgatccccg cgccgcgctc gtggctctcg accgcaccct
     2941 ggcgcccggc gggatcgtca tcgtctcggt gcccaacgtg gcccacctct gggtccggct
     3001 ctcgctcctg gccggccggt tcgactacgc ggaccgcggc atcctcgacc gcacgcacct
     3061 gcgcttcttc acccggcgga ccctggtggc cttcctccac tcggccggcc tcgcggtcgc
     3121 cgagctgcgg gtcaccccgg tgccgttgcc cctcgtggtg ccaccgcgct ggcacggcgc
     3181 cggcctgcgc ggactccacg acctgagcgc gcgggtcgcg cggctctggc cgggcggcct
     3241 cgcctaccag ttcgtcgcgg tgtgccgcgt cgagggccta ccctgatgga gcggcccgcc
     3301 caggtcgagc gccccagggt cgcgcccaag gtggtagtgg tcatgcccgc ctacaacgcg
     3361 ggccgcaccc ttcgcatgac gtacgaagaa ttgccgaagg acgcggtgaa cctggtgatc
     3421 ctggtcgacg acggctccac cgacaccacg ctcgaggtgg cgcgggacct ggggctcgag
     3481 atcttcgtcc acgaccgcaa ctacgggtac ggggcgaacc agaagacctg ctacaccgag
     3541 gcgctcaagg cgggcgccga catcgtggtg atggtccacc cggactatca gtacgatccc
     3601 acgctcgtgc cccagatcat cgccccgatc gtgcggggcg acgccgacct cgtgctgggc
     3661 tcgcggctcg cgagcggcgg ctccgcggtc cagcagggca tgccgtggtg gaagttcctc
     3721 gccaaccgct tcctcaccga ggtggagaac gtggccttcg gcctgcgcct ctcggagtac
     3781 cacaccggct atcgcgcgtt cagccgggcc gtgctcgagg acgtgaactt ccgcatgaac
     3841 tccgacggct tcgtgttcga ccaggagatc gtggcgcagg tggtgtcgac cggcttccgc
     3901 atcgcggaga tcccggtccc ggtgcgctac ttcccggagg cctcttccgc cagcttcgcc
     3961 gcctcctgcg tgtacgggct caagatcctc tgggtggtgg cccgctacct gctgcatcgc
     4021 tccgggatca agcgatcgag gcggctcgcc tcgatcgtgc atcggtatca ccgctaccgg
     4081 tgatcgcccg cctcctcgcc gtcgcggccg cggtcgcggc ggcgggcctc ggcctggcgc
     4141 tcgtcctgtg gaccggccgg cgggagcact ggccgtggcg ccccgagcac ttcttcctgc
     4201 tgctgctggg cctcctggcc ctgcgcgtcc tgatcgcgcc gcgcccgatc cccgcgctgc
     4261 gcccgcgccg ggtggtcgcg gtcggcgtgg cggtctacgc gctcctgttc tccttcgtga
     4321 cggtcacccg ccacttcacg ttcgcgaccc acgccctcga tctcggctac tacgtgcagc
     4381 tcggctggaa cctcgcacgc ggggccgggc cctacgtgag cctgcccgag atgcacgcct
     4441 ggggcgacca cctctccccg atcatgtacg ccttcgtgcc cgccttctgg atcgcccccg
     4501 ggccggtggt gctgctgatc gcgcagtcgg ccgcgctggc cctgggcggc gccgcggtgt
     4561 tcggcctggc ccgcgcccgc ctcggcgacg agcgaccggc cgcggccttc gcgctgctct
     4621 acctggccaa cccgtcgctg caggggatca acgtgcgcga ctttcacacc gcggccctcg
     4681 ccatcccgct gctgctggcc gccgcgtggg cggtggaggc gggccggccc tggtgggcgc
     4741 tcgccccggc cgcgctcacc cttctctgcc gcgaggacgc ggccctgccg gtcatcggtc
     4801 tgggggcctg gatggcgctg gcccgtcggc gctgggcccc cggcgccatc gcggcggtgg
     4861 gcgccctggc ggtgctcgcg atcgacgtgc gcttcatcgt gcccgcgtat cgcggcgagc
     4921 cgtattccca tctcgggcgc tacgcgcagc tcggcgactc cctcggggcg atcgtcacga
     4981 ccatcgtcct gcacccgctt cacaccctcg cctcgctgat caccgccgac cggatgctct
     5041 acctggccgc gatgctcgcc ccgctcgggc tcttgcccct cctcggcggc tgggacctcg
     5101 tcggcgtcct gcccgggctg gcccagaacc tcctgagcgt cgaccccgtc ctctacaact
     5161 accgcgccca gtaccaggcc ttcgtcctgc ccttcctggt cctggccgcg atcggcggct
     5221 acgcgcgcct cgagcggcgg cggccggggc actggccggt cgcggtcctg gtggcggcga
     5281 tggtggtgag cctcgcgctc gactcgcgca ccgcgaacaa cctcgcggtc gcgcgcttct
     5341 ggcccacgcc cgcacagatg gccgcctacc gggtgctcgc cgcggtccca cccgaggtcg
     5401 cggtgaccgc gcaggatccc tacgttcccc acctgagcct gcggccgcgg gtcttcgtct
     5461 tcccggtggg catcgacaag agcgactacg tgctggtcga cctcgaga
//
LOCUS       DATMCV010000950         5936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_586636, whole genome shotgun sequence.
ACCESSION   DATMCV010000950 DATMCV010000000
VERSION     DATMCV010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586636"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /gene="ftsA"
                     /locus_tag="VNU03_21720"
     CDS             complement(<1..707)
                     /gene="ftsA"
                     /locus_tag="VNU03_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWP77156.1"
                     /translation="MARSRVRQVIAGLDVGTTKICCVIADWSPVGNLDIVGVGTSPSR
                     GLRKGVVVNIDSTVESIKHAVAEAEEMAGVEIGSVIAGVAGGHIRGINSRGVVAVSGK
                     HREVSQADIDRALDAARAVNLPQDREIIHVLPQTFLVDDQDGVKEPLGMSGVRLEVEV
                     HLVTGATTSVRNVVRSVNRAGLQVQDLVLEPLASAEAVISTEEKELGILLIDLGGGTT
                     DVALFRDGAVWHTSSLPL"
     gene            complement(716..1579)
                     /locus_tag="VNU03_21725"
     CDS             complement(716..1579)
                     /locus_tag="VNU03_21725"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWP77157.1"
                     /translation="MRTHSAVITPRAGRGRLADLAERGPAMERQRVSRARMARRVTRR
                     VRWVTTRVVPIAIALAVLGAGGLGALWMLTSPRFTVTEVAVTGASRLRPEDVVAASGI
                     GPGTNLFRLDRGAVVARLEALPLIRRAEMVRRFPNQVTLAVEERRPFTLAHTGRLHWI
                     DEQGVNLGPETRAVAPKAPVITGLAPADLAADGGPSARVAAGIALLRVLLRSETTLLQ
                     QISEIDVSRPDGPVLYTVDGIEVRLGADDWEARLGRLQGVLAQVRSGGEAVGTIDLRF
                     RDQVVLKTVTR"
     gene            complement(1576..2457)
                     /gene="murB"
                     /locus_tag="VNU03_21730"
     CDS             complement(1576..2457)
                     /gene="murB"
                     /locus_tag="VNU03_21730"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWP77158.1"
                     /translation="MLGEIRGEVRFKEPLGFHTSLRIGGPADIFIVPQDVADIRCALS
                     FAEREQLSIDVIGGGNNLLVSDRGVRGVVLKLEGCLGRAEFNGEEAVAGAGVSLSALI
                     REAAALNLGGIECLVGVPATVGGAVAMNAGTPDGGIADFISAVYFLQPDGTLGEFKPG
                     AASFSYRMFAAPQGSVLIGARLQLHRRPFAEIQKDIKQRLKQKKATQPLALASAGWVW
                     KNPQGETASRLIEKVGLKGKRLNGAEISAKHANFIVNRGGAAATDIKALMQLTLDRVR
                     NHFGITLEPEMKTMGEI"
     gene            complement(2461..3867)
                     /gene="murC"
                     /locus_tag="VNU03_21735"
     CDS             complement(2461..3867)
                     /gene="murC"
                     /locus_tag="VNU03_21735"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWP77159.1"
                     /translation="MFKKYQHIHFVGIGGVGMSGIAEVLLTLGYRVTGSDARRSETVE
                     RLERLGAKVYVGHAAAQVEGAHVVVSSSAVARDNVEIVAARQRGVPVIARAEMLAELM
                     RLKYGIAIAGTHGKTTTTSMVAAVLGAGGFDPTVVVGGRVHGLGTNARLGQGEFLVAE
                     ADESDGSFLRLTPTIAVVTTVDAEHLDHYPDLDAIVAAFLAFANKVPFYGAVVVCLDD
                     PNLQRMIPRMMDKRVVTYGLEAGADVTARRLQFAEMRSTFEVVHRGTSLGTMNLQIPG
                     RHNVLNALAATAVALDLEMPFPKIQTALAGFAGVQRRFQVRGTARDVLVVDDYGHHPA
                     EIRATLAAAKAGFDRRVITVFQPHRYSRTQHLRDDFLTAFYQSDVLIVMDIYAAGEAP
                     IPGVHARDLADGIAAHGHREVLYLGNDRAAIIDYLCESTRAGDLVLTLGAGDVGQLGG
                     ELLQRLGDDDPDTSSKRR"
     gene            complement(3867..4967)
                     /gene="ftsW"
                     /locus_tag="VNU03_21740"
     CDS             complement(3867..4967)
                     /gene="ftsW"
                     /locus_tag="VNU03_21740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWP77160.1"
                     /translation="MPRKLTPDLPLFGVVVALVCVGVVMVYSASAIVAADRFHDPFFF
                     LKKQLFWAVLGLGCLWGGMLLDYRNLERVVMPLLIVSFVLLVLVLVPPFGQSINGTRR
                     WFHLGPASFQPVELAKFALVLYLASFLTRRQEVLGRFSEGLLPVLLVAGGMAALTLLQ
                     PDLGNSLALVILTLVLAYLAGARVQHMALIAGAALPVVIALIALKPYRWRRMVAFMNP
                     WDDPQGSGFQIIQSFLALGSGGWLGVGLGDSKQKLFYLPEPYTDFIFAIIGEELGLLG
                     ATVIVALFALLIWRGLRIGLHAPDPFGAFLGLGLTIMLATQTIVNLGVVTGTLPTKGL
                     PLPFISFGGSALLMTMFSAGVLLNISQHGRAA"
     gene            complement(4967..>5936)
                     /gene="murD"
                     /locus_tag="VNU03_21745"
     CDS             complement(4967..>5936)
                     /gene="murD"
                     /locus_tag="VNU03_21745"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWP77161.1"
                     /translation="LRTQVRPVMVGGNIGTPLAEHALEFPGDGIVVAETSSFQLDTTD
                     RFRPRVAVVLNVTPDHLDRHGTFERYVDAKARIFANQTATDCAVLNADDPVTAGLAGR
                     ARGRVIWFSRQSTLPHGVFIYDGWIVAKLNGSTERICPVTAITLRGQHNVENVLAATA
                     CALWTGMAPAAIRRGIAAFRGVPHRIERVHDDRGVVYYNDSKGTNVDSTIKALESFTE
                     PIVLIAGGKGKGQDFGPLAAAARGRVRRAILIGQDGPQIRAALEPAGVPGEDAESMED
                     AVRHARDAARVGDVVLLSPACASFDMFRNYEHRGDVFKAVVRALLG"
BASE COUNT          889 a         2137 c         2080 g          830 t
ORIGIN      
        1 agcggcaggc tgctggtgtg ccacaccgcc ccgtcgcgga agagggccac gtcggtggtg
       61 ccgccgccca ggtcgatgag gaggatgccc agctccttct cctcggtgga gatgaccgcc
      121 tccgcggagg cgagcggctc cagcaccagg tcctgcacct gcaggccggc gcggttgacg
      181 ctccggacca cgttgcgcac cgaggtggtg gcgccggtga cgaggtgcac ctcgacctcg
      241 aggcgcaccc ccgacatgcc gagcggctcc ttcaccccgt cctggtcgtc gaccaggaag
      301 gtctggggca gcacgtggat gatctcgcgg tcctggggca ggttcaccgc ccgggccgcg
      361 tcgagcgcgc ggtcgatgtc ggcctggctc acctcgcggt gcttgcccga gaccgccacc
      421 accccgcggc tgttgatgcc ccggatgtgg ccgcccgcga cccccgcgat caccgagccg
      481 atctcgacgc ccgccatctc ctcggcctcg gccaccgcgt gcttgatcga ctccaccgtc
      541 gagtcgatgt tgaccaccac gcccttgcgc aggccgcggg acgggctcgt ccccacgccc
      601 acgatgtcga ggttgcccac cggcgaccag tcggcgatca cgcagcagat cttggtcgtg
      661 cccacgtcca gcccggcgat gacctgccgc actcgggatc tggccattgc cctcctcacc
      721 tcgtcacggt cttgaggacc acctgatcgc ggaatctcag atcgatcgtg cccaccgcct
      781 caccgcccga ccgcacctgg gcgagcacgc cctgcagccg gcccagccgc gcctcccagt
      841 cgtcggcgcc cagccggacc tcgatgccgt ccaccgtgta gagcaccggg ccgtccggcc
      901 ggctcacgtc gatctcggag atctgctgga gcagcgtggt ctccgagcgg agcaggacgc
      961 ggagcagggc gatgcccgcg gcgacgcgtg cggacggccc gccgtcggcg gccaggtcgg
     1021 cgggggcgag gccggtgatg accggagcct tgggtgccac cgcgcgtgtc tccgggccca
     1081 ggttcacccc ctgctcgtcg atccagtgca gccggcccgt gtgggccagc gtgaacggcc
     1141 gccgctcctc caccgcgagc gtcacctggt tcgggaagcg ccgcaccatc tcggcacgcc
     1201 ggatcagcgg gagcgcctcc aggcgcgcga ccaccgcgcc gcggtcgagc cggaacaggt
     1261 tggtgcccgg gccgatgccg gaggcggcca ccacgtcctc cgggcgcagc cggctcgccc
     1321 cggtgaccgc cacctcggtg accgtgaagc gcggcgaggt cagcatccag agcgcgccga
     1381 gcccgcccgc gccgagcacc gcgagcgcga tcgcgatggg caccacgcgc gtggtgaccc
     1441 agcgcacccg gcgggtgacc cgccgggcca tccgggcgcg ggagacccgc tgccgctcca
     1501 tcgcggggcc gcgctcggcc aggtccgcga gccggccccg cccggcccgc ggggtgatga
     1561 ccgcgctgtg tgttctcaaa tctctcccat ggtcttcatc tcgggctcga gcgtgatgcc
     1621 gaagtggttg cgcacccggt cgagggtgag ctgcatgagg gccttgatgt cggtcgcggc
     1681 agcgcccccg cggttgacga tgaagttggc gtgcttggcc gagatctcgg ccccgttcag
     1741 gcgcttgccc ttgagcccga ccttctcgat gaggcgcgag gcggtctcgc cctgcgggtt
     1801 cttccacacc cagcccgccg aggccagcgc gagcggctgg gtcgctttct tctgcttgag
     1861 ccgctgcttg atgtccttct ggatctccgc gaagggccgc cggtgcagct gcaggcgcgc
     1921 cccgatgagc accgagccct gcggcgcggc gaacatccgg tagctgaacg aggccgcccc
     1981 cggcttgaac tcccccagcg tgccgtccgg ttgcaggaag tagaccgcgg agatgaagtc
     2041 cgcgatgccc ccgtccggcg tgcccgcgtt catcgccacc gccccgccca ccgtggccgg
     2101 caccccgacc aggcactcga tgccgcccag gttcagcgcg gccgcctcgc ggatcagggc
     2161 cgagaggctc acccccgccc ccgccaccgc ctcctcgccg ttgaactcgg cgcggccgag
     2221 gcagccctcg agcttgagca ccacgccgcg gacgccgcgg tcgctgacca gcaggttgtt
     2281 gccgccgccg atgacgtcga tcgagagctg ctcgcgctcc gcgaacgaca gcgcgcagcg
     2341 gatgtctgcc acgtcctggg ggacgatgaa gatgtccgcc ggacctccga tgcgcaagga
     2401 ggtatggaag ccgagcggct ccttgaatcg gacctcgccc cggatctctc ccagcatgcg
     2461 ttatctcctc ttggatgacg tgtcgggatc gtcgtcgccg agccgctgga gcagttcccc
     2521 gcccagctgg cccacgtcgc cggccccgag agtgagcacg agatcacccg ctctggtgga
     2581 ttcgcacagg tagtcgatga tggccgcgcg atcgttgccc aggtacagca cctcacggtg
     2641 cccgtgggcc gcgatgccgt cggccaggtc gcgggcgtgc acgccgggaa tcggcgcctc
     2701 acccgcggcg tagatgtcca tgacgatgag gacgtcggac tggtagaacg cggtaagaaa
     2761 atcatcgcgt aggtgctgcg tccttgaata gcggtgcggc tggaacaccg tgatcacgcg
     2821 ccggtcgaac cccgccttgg cggcggcgag ggtcgcgcgg atctcggcgg ggtgatggcc
     2881 gtagtcgtcg acgacgagca cgtcccgggc ggtgccccgg acctggaagc ggcgctgcac
     2941 gcccgcgaag ccggccaggg cggtctggat cttcggaaac ggcatctcga ggtcgagcgc
     3001 gaccgcggtg gcggccagcg cgttgagcac attgtggcgg ccggggatct ggagattcat
     3061 cgtcccgagc gaggtcccac ggtgcaccac ctcgaaggtc gagcgcatct ccgcgaactg
     3121 gagtcgccgc gcggtgacgt cggcgcccgc ctcgaggccg taggtcacca cccgcttgtc
     3181 catcatcctc gggatcattc gctgcaggtt gggatcgtcc aagcagacga ccaccgcgcc
     3241 gtagaacggc accttgttcg cgaaggcgag gaacgcggcc acgatcgcgt cgaggtccgg
     3301 gtagtggtcg agatgctcgg cgtccaccgt ggtcaccacc gcgatggtgg gggtgagccg
     3361 gaggaaggag ccgtcggact cgtcggcctc cgcgaccagg aactcgccct ggccgaggcg
     3421 ggcgttggtg ccgagcccgt gcacgcggcc gccgaccacc acggtgggat cgaagccgcc
     3481 cgcccccagc accgcggcca ccatcgaggt ggtggtggtc ttgccgtggg tgcccgcgat
     3541 cgcgatgccg tacttgagcc gcatgagctc cgcgagcatc tcggcccgcg cgatcaccgg
     3601 gacgccgcgc tgacgcgcgg ccactatctc cacgttgtcg cgcgccaccg ccgaggaaga
     3661 gaccaccacg tgggccccct cgacctgcgc cgccgcgtgc cccacgtaga ccttggcccc
     3721 cagccgctcg aggcgctcca ccgtctcgct gcgccgggcg tcggagccgg tgacccggta
     3781 gccgagcgtg agcaggacct cggcgatgcc gctcatgccg acgccgccga tgcccacgaa
     3841 gtggatgtgc tggtacttct tgaacatcag gccgctctcc cgtgctgcga gatgttgagc
     3901 agcacgccgg ccgagaacat ggtcatgagc agcgcggagc ccccgaagga gatgaacggc
     3961 agcggcagcc ccttggtggg cagcgtcccg gtcaccacgc ccagattcac gatggtctgg
     4021 gtcgcgagca tgatggtgag cccgagtccc aggaaggcgc cgaagggatc gggcgcgtgc
     4081 aggccgatgc gcaggccgcg ccagatgagc agggcgaaga gggccacgat gacggtggct
     4141 ccgagcaggc cgagctcctc gccgatgatc gcgaagatga agtcggtgta aggctccggc
     4201 aggtagaaga gcttctgctt ggagtcgccg agcccgaccc cgagccagcc gcccgagccc
     4261 agcgcgagga acgactggat gatctggaat ccgctgccct ggggatcgtc ccacgggttc
     4321 atgaacgcga ccatccgccg ccaccggtag ggcttgagcg cgatcagggc gatcaccacc
     4381 ggcagcgccg cgcccgcgat gagcgccatg tgctgcaccc gcgcgccggc caggtaggcg
     4441 agcaccaggg tcaggatcac cagggccagg ctgttgccga ggtcgggctg gagcagggtc
     4501 agcgcggcca tgccgcccgc caccagcagc accggcagca agccctcgct gaagcgcccg
     4561 aggacttcct gtcgcctcgt caggaacgag gccaggtaga gcacgagcgc gaacttggcg
     4621 agctccaccg gctggaagga cgccgggccc aggtggaacc agcgccgggt gccgttgatc
     4681 gactggccga acggcggcac cagcaccagc accagcagca cgaaggagac gatcaggagc
     4741 ggcatcacga cccgctccag gttccggtag tcgaggagca tgccgcccca gaggcagccg
     4801 agccccagca ccgcccagaa gagctgcttc ttgaggaaga agaacgggtc gtggaagcgg
     4861 tcggccgcca cgatcgcgct cgccgagtag accatcacca cgcccacgca cacgagggcc
     4921 accaccactc cgaagagggg gagatcgggc gtgagcttgc ggggcatcag ccgagcagcg
     4981 cccgcaccac ggccttgaac acgtcgccgc gatgctcgta gttgcggaac atgtcgaacg
     5041 aggcgcacgc gggcgagagc agcaccacgt cgcccacccg cgccgcgtcg cgggcgtgcc
     5101 gcaccgcgtc ctccatcgac tccgcgtcct cgccggggac gccggccggc tcgagcgcgg
     5161 ctcggatctg gggcccgtcc tggccgatca ggatcgcccg ccgcacccgc ccgcgggccg
     5221 ccgccgcgag ggggccgaag tcctgcccct tccccttgcc gcccgcgatg agcacgatcg
     5281 gctcggtgaa gctctcgagg gccttgatgg tggagtcgac gttggtgccc ttggagtcgt
     5341 tgtagtagac gacgccgcga tcgtcgtgca cgcgctcgat gcggtggggg accccgcgga
     5401 aggcggcgat gccgcggcgg atcgcggcgg gggccatccc ggtccacagc gcgcacgcgg
     5461 tggccgcgag cacgttctcc acgttgtgct ggccgcgcag ggtgatcgcg gtcaccgggc
     5521 agatccgctc ggtggagccg ttgagcttgg ccacgatcca cccgtcgtag atgaacaccc
     5581 cgtggggcag cgtgctctgc cggctgaacc agatcacccg tccccgcgcc cggcccgcga
     5641 gcccggcggt gaccgggtca tccgcgttca gcaccgcgca gtcggtcgcg gtctggttgg
     5701 cgaagatgcg cgccttggcg tccacgtagc gctcgaacgt gccgtggcgg tccaggtggt
     5761 ccggggtcac gttcaggacc accgcgaccc gcgggcggaa gcggtcggtg gtgtcgagct
     5821 ggaagctgga cgtctccgcg accacaatgc cgtcgcccgg gaactcgagc gcgtgctcgg
     5881 cgagcggggt gccgatgttg ccgcccacca tcaccggccg cacctgggtg cgcagc
//
LOCUS       DATMCV010000951         5188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_118290, whole genome shotgun sequence.
ACCESSION   DATMCV010000951 DATMCV010000000
VERSION     DATMCV010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118290"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1748
                     /locus_tag="VNU03_21750"
     CDS             78..1748
                     /locus_tag="VNU03_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWP77162.1"
                     /translation="MTPEERARFEEARRQWEERTLKPALGRGPERPGPFADSSGAPLE
                     RLHTPADVDGVDYVRDLGFPGEFPFTRGVQPTMYRGRLWTMRQYSGFGSATETNRRFR
                     YLLEQGQGGLSVAFDLPTQMGYDADHEVARSEVGKVGVAISSVEDMAALFEAIPLDRA
                     STSMTINATASILLCLYIAVAERQGVPSERISGTVQNDILKEYVARGTYIFPPGPSMR
                     LITDTFAFCRERVPRWNTISISGYHIREAGSTAAQEVAFTLANGIAYVTAAQEAGLKV
                     DEFAPQLSFFFNAHNNLIEEVGKYRAARRLWARIMRERFDARDPRSQMLRFHAQTAGS
                     MLTAQQPENNIVRVAVQALAAVLGGCQSLHTNSMDEALSLPSEQAVRIALRTQQVLAH
                     ESGVADVADPLGGSYTVERVTRRIEEEAEAYIAKIDGLGGSVHAIPFMQREIQEAAYR
                     YQQEIEGKQRVVVGVNEFVMDEPPPANLFQVDPAVGAALAERLAELRRRRDGDLAARA
                     LDAVDRAARGRDNLLPPILAAVRAHVTLGEICDTLRRVFGTHQPSVVF"
     gene            1750..2550
                     /locus_tag="VNU03_21755"
     CDS             1750..2550
                     /locus_tag="VNU03_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77163.1"
                     /translation="MRWPRLAALPLLVLALHTPVGAGTADQVGATFGLLIQDVVAAFP
                     PVEGLVVQLDGDRLFLDLTEKNGVLPGQEFSVFRKGEVFRHPINGRPLGRYEDVLGYA
                     QILRVHPQYSEALFVPTEGKPPVQPEDGIRITKGRIRVAVAPATDLTKANADLRRVPF
                     MLAHALELTKRFQVADPATVQEHLLGQKTRSEELLVSPGKARSSGKTLEVTGWLVPVL
                     IERRGVTYLDVTWVSAVTGTALFSRRAALSRTEGTAEQRFPWEPLPTD"
     gene            2587..3333
                     /locus_tag="VNU03_21760"
     CDS             2587..3333
                     /locus_tag="VNU03_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77164.1"
                     /translation="MLWPIMKKTSRRVQWIVLAGILMLVTSGCGGRGWREYLGLASPS
                     DASVALRPAEPKWLLIKNPRCCDVASEPEYIWVEEDKVPTTVKSFMFGQSTLLAAPEV
                     VSKYGPPPGGGRISARQGGAYKVENVVPTVQTSRSASAVVASTSRTDAATGVAAQPAT
                     RGYVVFVDTSRVVIDLAAPDGVKLGTNVSVRRDKIAIVHPITGELLGELDEEVATGRV
                     IEVRDKFSVVEVQKAAAGAQVQVKDRVVLR"
     gene            3357..4358
                     /locus_tag="VNU03_21765"
     CDS             3357..4358
                     /locus_tag="VNU03_21765"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWP77165.1"
                     /translation="MKAPTPLEALLKDRVALAIGGDLGAIEAAIGREIASPVRLIHEM
                     GEFIAGAGGKRLRPILLLIAARLAGYQGPRAVRMGCVVELLHTATLIHDDVVDQAPLR
                     RGRPSANARWGDDASILVGDHLYSKSFALMVEDGDPRVLETLARATVSMTEAEVFQLE
                     RKRSGTTGEADYLRIITQKTASFMSACCRIGGLLGRADPEQVEGLTRYGLDIGIAFQI
                     SDDSLDFVADQARLGKAVGADLKEGKRTLPLIATVERAGPADRDRIQALLRQPALSPD
                     EIEEIRRLVVKHAGVEYALERAHQYAQSAKDALRGFPDSEDKETLVLVADFVVDRDR"
     gene            4430..>5188
                     /locus_tag="VNU03_21770"
     CDS             4430..>5188
                     /locus_tag="VNU03_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWP77166.1"
                     /translation="MPIITLTTDFGQRDPFVGIMKGVILSICPTARLVDLTHDVEPQD
                     IRGASLALEAALPFFPDGTVHLAVVDPGVGSRRRPLAVRAGRHYFVGPDNGVLTPALR
                     GPRWTAVALTAPEYRLPEVSRTFHGRDVFAPAAAYLAAGVPLERLGAVAADPVLEPIP
                     GSRLEGDALVGEVLAVDRFGNLLTSIEAGRLAALAGDGPVAVEVSGQPVAGLVEAYAD
                     GSDGRPAAIIGSTGRFEIFVRGGSAHQMLGAGRGA"
BASE COUNT          746 a         1880 c         1832 g          730 t
ORIGIN      
        1 cctcgaggtc ctccacacca agcgcgacag caacccgccc aagaagcacg ggaatatccc
       61 gctatgagcc ggtcgtgatg accccggagg agcgcgcgcg cttcgaggag gcccgccggc
      121 agtgggagga gcggacgctc aagcccgcgc tgggtcgggg tcccgaacgg ccggggccgt
      181 tcgcggattc gagcggcgcc ccgctcgagc gcctgcacac ccccgccgac gtcgacggcg
      241 tcgactacgt gcgcgatctc ggcttcccgg gcgagttccc gttcacccgc ggcgtgcagc
      301 ccaccatgta ccgcgggcgg ctctggacca tgcgccagta ctcgggcttc ggctcggcta
      361 ccgagaccaa ccgccggttc cgctacctgc tcgagcaggg gcagggcggt ctcagcgtgg
      421 ccttcgacct gcccacccag atgggctacg acgcggacca cgaggtcgcc cgcagcgagg
      481 tcggcaaggt cggggtcgcg atctcgagcg tggaggacat ggccgcgctg ttcgaggcca
      541 tcccgctcga ccgtgcctcc acctcgatga ccatcaacgc gaccgcctcg atcctgctct
      601 gtctgtacat cgcggtggcc gagcggcagg gggtgccgtc ggagcgcatc tccggcaccg
      661 tccagaacga catcctcaag gagtacgtgg cccgcggcac ctacatcttc ccccccggcc
      721 cgtcgatgcg gctcatcacc gacaccttcg cgttctgtcg ggagcgggta ccgcgctgga
      781 acaccatctc gatctcgggc taccacatcc gcgaggccgg ctccaccgcg gcccaggagg
      841 tggccttcac cctcgccaac ggcatcgcct acgtgaccgc ggcccaggag gccgggctca
      901 aggtcgacga gttcgcgccc cagctgtcgt tcttcttcaa cgcccacaac aacctgatcg
      961 aggaggtcgg gaagtaccgg gccgcgcgcc ggctgtgggc ccgcatcatg cgcgagcgct
     1021 tcgacgcccg cgacccgcgc tcccagatgc tgcgcttcca cgcccagacc gcgggcagca
     1081 tgctcaccgc gcagcagccc gagaacaata tcgtgcgggt ggcggtgcag gccctcgccg
     1141 cggtgctcgg aggctgccag tcgctgcaca ccaactcgat ggacgaggcg ctctccctgc
     1201 cgagcgagca ggcggtgcgc atcgcgctgc ggacccagca ggtgctggcc cacgagtccg
     1261 gggtggccga cgtggccgac cccctgggcg gctcctacac cgtggagcgg gtcacccggc
     1321 ggatcgagga ggaggcggag gcctacatcg cgaagatcga cgggctcggc ggctcggtgc
     1381 acgcgatccc cttcatgcag cgcgagatcc aggaggcggc gtaccgctac cagcaggaga
     1441 tcgagggcaa gcaacgcgtg gtggtgggcg tcaacgagtt cgtcatggac gagccgccgc
     1501 ccgccaatct cttccaggtc gatcccgcgg tgggcgcggc cctcgccgag cgcctggccg
     1561 agctgcgccg gcgtcgcgac ggcgacctgg ccgcgagggc gctcgacgcc gtcgaccggg
     1621 ccgcccgcgg ccgcgacaac ctgctgcccc ccatcctggc cgcggtgcgg gcccacgtga
     1681 ccctcggcga gatctgcgac acactccggc gggtgttcgg cacgcaccag ccgtcggtgg
     1741 tcttctaggg tgcgctggcc tcggctcgcc gcgctgccgt tgctggtcct ggcgctccac
     1801 acgccggtcg gcgcgggtac cgcagaccag gtgggcgcca ccttcggcct cctcatccag
     1861 gacgtggtgg ccgcgttccc gcccgtcgag ggcctggtgg tgcagctgga tggtgatcgg
     1921 ctcttcctcg acctcaccga aaagaacggt gtcctgcccg gccaggagtt ctcggtgttc
     1981 cgcaaaggcg aggtcttccg ccatccgatc aacggccggc cgctcggccg ctacgaggac
     2041 gtcctgggct atgcccagat cctccgggtg cacccgcagt actccgaggc gctgttcgtg
     2101 cccacggagg gcaagccgcc ggtccagccc gaggacggga tccggatcac gaaagggcgc
     2161 atccgggtgg cggtggcgcc ggccaccgat ctgaccaagg cgaacgcgga cctgcgccgg
     2221 gtgccgttca tgctggcgca cgccctcgag ctgacgaagc gcttccaggt cgcggatccc
     2281 gccacggtgc aggagcacct gctgggccag aagacgcgca gcgaggagct gctcgtgagc
     2341 cccggcaagg cccgcagctc cggcaagacc ctggaggtca ccggctggct ggtgccggtg
     2401 ctcatcgagc gccgcggggt cacttatctc gacgtcacct gggtatccgc ggtcaccggg
     2461 accgccctgt tctcacgccg ggcggccctg agccggaccg agggcacggc cgagcagcgg
     2521 ttcccgtggg agcccctccc gaccgactaa acccccggaa gccacgggga agacgggggt
     2581 tctgatatgc tgtggcccat tatgaagaag acttctcgac gcgtgcagtg gatcgtcctg
     2641 gccggcatcc tgatgctcgt cacctccggg tgcggcggcc ggggatggcg agagtacctc
     2701 gggttggcgt cgccgagcga cgcatcggtc gcccttcggc cagcggagcc caagtggctg
     2761 ctgatcaaga acccgcggtg ctgcgacgtg gcgagcgagc ccgagtacat ctgggtcgag
     2821 gaggacaagg tccccacgac cgtcaagtcg ttcatgttcg gccagtccac cctcctcgcg
     2881 gcacccgagg tcgtctccaa gtacggccca cccccgggcg gcggacggat cagcgcccgc
     2941 cagggcgggg cctacaaggt ggagaacgtg gtcccgacgg tgcagacctc gcgtagtgcc
     3001 tccgcggtgg tggccagcac gagccggacc gacgccgcca ccggcgtcgc ggcgcagccg
     3061 gccacgcgcg gctacgtcgt gttcgtggac acctcccggg tggtcatcga cctggccgcg
     3121 cccgacgggg tcaagctcgg caccaacgtg agcgtgcggc gcgacaagat cgcgatcgtc
     3181 caccccatca ccggcgagct gctcggcgag ctggacgagg aggtggccac cgggcgcgtg
     3241 atcgaggtcc gcgacaagtt ctcggtggtc gaggtgcaga aggccgccgc gggggcgcag
     3301 gtccaggtca aggaccgggt ggtgctccgg taaccggcgg gctcccgcct ccttcggtga
     3361 aagcgcccac tccgctcgaa gcgcttctca aggaccgcgt cgccctggcc atcggcgggg
     3421 acctcggcgc gatcgaggcc gccatcgggc gcgagatcgc ctcgccggtg cgcctgatcc
     3481 acgagatggg cgagttcatc gcgggggcgg ggggcaaacg gctccgcccg atcctgctcc
     3541 tgatcgcggc gcgcctggcc ggctaccagg gcccgcgcgc ggttcgcatg gggtgcgtgg
     3601 tggagctgct gcacaccgcg accctcatcc acgacgacgt ggtcgaccag gccccgctcc
     3661 gccgcgggcg gccgtccgcc aacgcgcgct ggggcgacga cgcctcgatc ctggtgggcg
     3721 accacctcta ctcgaagtcg ttcgccctga tggtcgagga cggcgatccg cgcgtgctcg
     3781 agaccctcgc gcgggcgacc gtgtcgatga ccgaggccga ggttttccag ctcgagcgca
     3841 agcgtagcgg caccaccggg gaggccgact acctgcggat catcacccag aagacggcct
     3901 ccttcatgtc ggcgtgctgc cggatcggcg gtctcctggg gcgcgccgac cccgagcagg
     3961 tcgagggctt gacccgctac gggctggaca tcggcatcgc cttccagatc agcgacgact
     4021 cgctcgactt cgtcgcggac caggcgcgcc tcggcaaggc ggtgggggcc gacctgaagg
     4081 agggtaagcg caccctgccg ctcatcgcca ccgtcgagcg ggccggcccc gcggatcgcg
     4141 accgcatcca ggccctgctc cggcagcccg cgctcagccc cgacgagatc gaggagatcc
     4201 gccggctggt ggtcaagcac gccggggtcg aatacgcgct cgagcgggcc caccagtacg
     4261 cgcagagcgc caaggacgcg ctccggggat tccccgactc cgaggacaag gaaacgctgg
     4321 tcctcgtcgc ggacttcgtg gtcgatcgcg accgctagcc tctccctctc ccctcaggga
     4381 agagggcagg gtgagggggc ggccgccggg ccggggcgag ggaaaaccga tgcccatcat
     4441 caccctgacc acggatttcg ggcagcgcga tcccttcgtc gggatcatga agggcgtcat
     4501 cctctcgatc tgcccgaccg cgcgcctggt ggacctcacc cacgacgtgg agccgcagga
     4561 cattcgggga gccagtctcg cgctcgaggc ggcgcttccg ttcttcccgg acggcaccgt
     4621 gcatctggcg gtggtcgatc ccggcgtcgg cagccgccgg cgcccgctcg cagtccgggc
     4681 gggccgacac tacttcgtcg gccccgacaa cggggtgctc acccccgcgc tccgcggccc
     4741 ccgatggacc gcggtcgcgc tcaccgctcc ggagtaccgg ctgcccgagg tgagccgcac
     4801 cttccacggc cgtgacgtgt tcgcgccggc cgcggcctac ctggcggccg gcgttccgct
     4861 cgagcgcctc ggcgcggtgg cggcggaccc ggttctcgag cccatccccg gcagccgtct
     4921 cgagggcgac gcgctggtcg gcgaggtgct cgcggtcgac cgcttcggca acctgctcac
     4981 ctcgatcgag gcgggccggc tcgcggcgct cgccggcgac ggacccgtcg cggtggaggt
     5041 gtcgggccag ccggtcgcgg ggctcgtcga ggcctacgcc gacgggtcgg acgggcggcc
     5101 cgcggcgatc atcggctcga ccggacgctt cgagatcttc gtgcggggcg gcagcgctca
     5161 ccagatgctg ggcgcgggtc ggggcgcg
//
LOCUS       DATMCV010000952         4317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_118314, whole genome shotgun sequence.
ACCESSION   DATMCV010000952 DATMCV010000000
VERSION     DATMCV010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118314"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VNU03_21775"
     CDS             complement(<1..214)
                     /locus_tag="VNU03_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77167.1"
                     /translation="MFGSAIIDIAIGLVFVYLVLSLVVTAAQEVLETIIKLRGAHLAK
                     GIEKLLGEARAKEFFASAAVAALSPDR"
     gene            297..1103
                     /gene="nagB"
                     /locus_tag="VNU03_21780"
     CDS             297..1103
                     /gene="nagB"
                     /locus_tag="VNU03_21780"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008983987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HWP77168.1"
                     /translation="MLVVLAADTAALDREAARIVAHRVREKPAITLGLASGSTPLGMY
                     RELIRLHREEGLDLSAVTTFNLDEYLGLAPSHPRSFHRFMREALFDHVNIAPERIHVP
                     DGAVSGDLEAYCAEYERRIARAGGIDLQVLGIGRDGHVGFNEPTSSLASRTRVKTLTL
                     ATIEDNRRVFGEGEPVPECAITMGIGTILEAREILLLGAGPTKRDAVAKAVEGPLTAS
                     VTASALQLHPRVTVLLDREAAAGLRHLDYYERVMRTTAAITPGRAARASD"
     gene            complement(1100..1372)
                     /locus_tag="VNU03_21785"
     CDS             complement(1100..1372)
                     /locus_tag="VNU03_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77169.1"
                     /translation="MDPEREDIARWLDEGRELLAMLHEQVEHLAQDNERLRAELARID
                     ELQAVIVRVNQDNDRLRAERDELLATFRRVADVVEQLRESRSRGQA"
     gene            complement(1442..2209)
                     /locus_tag="VNU03_21790"
     CDS             complement(1442..2209)
                     /locus_tag="VNU03_21790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77170.1"
                     /translation="MVRYASYPSLEDRVVFVTGGGSGIGASVVEHLCAQRARVAFVDI
                     QREASDALVARVAARGLPAPAFIPCDLRDIAALRAAIAQVEARLGAVRVLVNNAAHDE
                     RHAIEAVTPEYWDDRFAVNLRHQFFAAQAVVPGMAAAGGGSIVNFGSTSWLVGQGGMP
                     AYLSAKAAVAGLTRALARDLGPRKIRVNSIVPGWIMTERQISLWLTPEGEEELMRRQC
                     LKRKLVPEDIARVVLFLAADDSDACTNQSYVVDGGWV"
     gene            2295..3209
                     /locus_tag="VNU03_21795"
     CDS             2295..3209
                     /locus_tag="VNU03_21795"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HWP77171.1"
                     /translation="MKPAEPITLRADGAGLTVAPAVGGAVTRHWLERDGTTWEWLRPT
                     PPEALRDGLPYQTAAFPLVPFSNRIREGRFTFRGRAVRLPLNRPPERHAIHGHGWQSA
                     WRAIAVGPADAQLEYRHPASAWPGPYRATQRFTLTASSLTVALTLTNEGDTPMPAGLG
                     WHPYFPRPPRATITADVRSVWLTDAETMPTTLGPPPPAADPARGVAVDAVALDNCFVG
                     WSRRAVIEWPERGASLAMTAGPPLDYLVVFTPPDRPFFCAEPVSHVTDAFNLADAGRP
                     DTGRRVLDPGATIAAAITLTGRAPAATG"
     gene            complement(3199..4197)
                     /locus_tag="VNU03_21800"
     CDS             complement(3199..4197)
                     /locus_tag="VNU03_21800"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77172.1"
                     /translation="MRTRRRFLGTLAVLPLAAPVIVAAQPAGRVPHVAVLNPGSATEA
                     PAVQREPFERGLRELGWRPGATVVIEYVYGDGSASQLATVTANLVRRGVEVIVARGPV
                     AIRAAREATTTVPIVMSAADDPIADGFVGTLARPGGNVTGIANIVSDLDGKRLDLLRE
                     AIPGLARVGVLTNPTMRPGRNQELIAALHAAARARGLELQIFEVTRTDEIPGAFVAIA
                     RARVGALLVRADTQVLEPNRSMVAAMAIRHRLPAMYPWHFYAEVGGLMAYASSIPGFH
                     HRSAMYVDRILKGTKPGDLPVERPTTFELMINLKTAGLLGLTLPASLLARADEVIQ"
BASE COUNT          612 a         1632 c         1500 g          573 t
ORIGIN      
        1 atcgatcggg cgacagcgcc gcgacggcgg cggacgcgaa gaactccttc gcccgcgcct
       61 cgcccaggag cttctcgatc cccttcgcga gatgcgcccc gcgaagcttg atgatggtct
      121 cgaggacctc ctgggcggcg gtgacgacca ggctcaggac caggtacacg aagacgaggc
      181 cgatcgcgat gtcgatgatg gcggacccga acatggtgcc tcctcgcggg cgggatcaag
      241 ccggctcccg cccttgatag aatagcccgc atgccccgac ccgcgcccgg atcgcgatgc
      301 tcgtagtcct cgccgcggac accgcggccc tcgaccgtga ggccgcgcgc atcgtggccc
      361 accgcgtccg cgagaagccg gcgatcaccc tggggctcgc gtcggggagt accccgctcg
      421 ggatgtatcg cgagctgatc cgcctccacc gcgaggaagg cctcgatctc tcggcggtga
      481 ccaccttcaa cctggacgag tacctcggcc tggccccgtc gcatccccgg agcttccacc
      541 gcttcatgcg ggaggcgctc ttcgatcacg tgaacatcgc gcccgagcgc atccacgtcc
      601 cggacggggc ggtgtccggc gatctcgagg cgtactgcgc ggagtacgag cggcggatcg
      661 cgcgcgcggg gggcatcgac ctgcaggtgc tcggcatcgg ccgggacggc cacgtgggct
      721 tcaacgagcc gacgtcgagc ctcgcctcgc ggacgcgggt caagaccctc accctcgcca
      781 ccatcgagga caaccggcgc gtcttcggcg agggtgagcc ggtgcccgag tgcgcgatca
      841 cgatgggcat cggcacgatc ctggaggcgc gggaaattct cctgctgggc gcgggcccca
      901 ccaagcgcga cgcggtcgcg aaagccgtgg aggggccgct gaccgcgtcg gtcaccgcct
      961 ccgcgctcca gctgcatccg cgggtgacgg tcctgctcga ccgggaggcc gccgcgggcc
     1021 tccgccacct cgactactac gagcgggtca tgcgcaccac cgccgcaatc acacccggcc
     1081 gcgcggcgcg cgcgagcgac taggcctgtc cccggctccg tgattcgcgg agctgttcta
     1141 ccacgtcggc gacccgacga aaggtcgcca gcagctcatc tcgctcggcc cgcagccggt
     1201 cattgtcctg attgacgcgc acgatgaccg cctggagctc gtcgatgcgc gcgagctcgg
     1261 cgcggaggcg ctcgttgtcc tgggcgaggt gctcgacctg ctcgtgcagc atcgcgagga
     1321 gctcacggcc ttcgtcgagc cagcgagcga tgtcctcccg ctccgggtcc atggtcaagt
     1381 ccgaggcacc cgcacgaagg ccatggccgg aggataaccc ctcggcgcgc cgtccgtcct
     1441 cctataccca gccgccgtcg accacgtagc tctggttggt gcaggcgtcg ctgtcgtccg
     1501 cggccaggaa gagcacgacg cgtgcgatgt cctccggcac cagcttgcgc ttgaggcact
     1561 gccggcgcat cagctcctcc tcgccctccg gggtgagcca gagggagatc tgccgctcgg
     1621 tcatgatcca gcccggcacg atgctgttga cccggatctt ccggggcccg agatcgcggg
     1681 ccagggcccg ggtgagcccg gcgaccgcgg ccttcgcgct caggtaggcc ggcatgccgc
     1741 cctgcccgac cagccacgag gtcgagccga agttgacgat ggagccgcct cccgccgcgg
     1801 ccatcccggg cacgaccgcc tgcgcggcga agaactggtg gcgcaggttc accgcgaagc
     1861 gatcgtccca gtactcgggc gtcaccgcct cgatggcgtg gcgctcgtcg tgcgccgcgt
     1921 tgttgacgag gacccggacc gctccgagcc gggcctcgac ctgggcgatc gccgcgcgca
     1981 gcgccgcgat gtctcgcaga tcgcacggga tgaacgcggg ggccggaagc ccgcgcgcgg
     2041 ccacgcgggc cacgagggcg tcggaggcct cgcgctggat gtccacgaag gcgacgcggg
     2101 cccgctgcgc gcacaggtgc tcgacgacgg aggcgccgat gccgctgccg ccgccggtca
     2161 cgaagacgac gcgatcttcc aggctcgggt acgacgcata tcggaccatg agcaccctcc
     2221 cgcgctagac tatgccacga ggacggggcc atggacgcaa cgccgaccac cgcgccacca
     2281 gtagcggccc cgcgttgaag ccggcggagc cgatcaccct gcgcgcggac ggcgccggcc
     2341 tcacggtcgc gccggcggtc gggggcgcgg tcacccgcca ctggctcgag cgcgacggca
     2401 cgacgtggga gtggctgcgc ccgacgccgc cggaggcgct gcgcgacggc cttccctacc
     2461 agaccgcggc cttcccgctc gtgcccttct ccaatcgcat ccgcgagggc cgcttcacct
     2521 ttcgcggccg cgcggtccgc ctcccgctga accggccccc ggagcgccac gcgattcacg
     2581 gtcacggctg gcaatcggcc tggcgcgcaa tcgcggtggg gccggccgac gcgcagctcg
     2641 agtaccgtca ccccgcctcc gcctggccgg ggccctaccg cgcgacgcag cggttcaccc
     2701 tcaccgcctc gagcctcacg gtggcgctga ccctcaccaa cgagggcgac accccgatgc
     2761 cggccgggct cggctggcac ccctacttcc cgcgcccgcc acgagcgacg atcaccgcgg
     2821 acgttcgctc cgtctggctc accgacgcgg agacgatgcc gaccacgctg ggcccgccgc
     2881 cgcccgccgc ggatccggcg cgaggggtcg cggtggacgc ggtggcgctc gacaactgct
     2941 tcgtgggctg gagccggcgc gcggtcatcg agtggcccga gcgaggcgcg agcctcgcca
     3001 tgaccgcggg gccaccgctc gactacctgg tggtcttcac gccgccggac cggcccttct
     3061 tctgcgcgga gccggtcagc cacgtgaccg acgcgttcaa cctggccgac gcggggcgcc
     3121 cggacaccgg gcgccgggtg ctcgatccgg gcgcgacgat cgcggccgcg atcaccctga
     3181 ccggtcgggc acccgccgct actggatgac ctcgtcggcc cgcgccagca gcgacgcggg
     3241 gagcgtgagc ccgagcagcc cggcggtctt cagattgatc atcagttcga acgtggtcgg
     3301 gcgctccacc ggcagatcgc cgggcttcgt ccccttcagg atgcggtcca cgtacatcgc
     3361 ggagcgatgg tggaatccgg gaatgctgct cgcgtacgcc atcaggccgc ccacctccgc
     3421 atagaagtgc cacggataca tcgcgggcag ccgatgccgg atcgccatcg ccgcgaccat
     3481 ggatcgattg ggctcgagga cctgggtgtc ggcccgcacc agcagcgcgc ccacccgggc
     3541 gcgggcgatg gccacgaacg cccccggaat ctcgtcggtc cgagtgacct cgaagatctg
     3601 cagctcgagc cctcgcgccc gcgcggcggc gtgcagcgcg gcgatcagct cttggttccg
     3661 gccgggccgc atcgtcggat tcgtgaggac gcccactcgc gcgagcccgg ggatcgcctc
     3721 ccggagcaga tcgaggcgct tgccgtccag gtccgagacg atgttggcga tgccggtgac
     3781 attgccgccc ggtcgggcca gcgtgccgac gaagccgtcc gcgatcgggt cgtcggcggc
     3841 cgacatgacg atggggacag tggtcgtcgc ctcgcgggcc gcccggatgg ccaccggccc
     3901 gcgggccacg atgacctcga cgccccgccg gacgaggttc gcggtcacgg tggcgagctg
     3961 gctggcgctc ccatcgccgt acacgtactc gatcaccacc gtcgcgccgg gccgccagcc
     4021 cagctcccgc agaccacgct cgaacggctc gcgctgcacc gcgggcgcct cggtggcgct
     4081 ccccgggttg agcaccgcga cgtgcggaac gcgccccgcg ggctgcgccg ccacgatgac
     4141 cggcgcggcg aggggcagca cagcgagagt gccgagaaac cggcgccggg tcctcatgcc
     4201 tctcgcgacc cgaccggctt cgccgcgcgc agccagcgcc agacctgctc cggggtgatc
     4261 ggcagcgagc ggaccacgac ccccaggtgc gcgagcgcgt cctccaccgc gttggcc
//
LOCUS       DATMCV010000953         6281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_317671, whole genome shotgun sequence.
ACCESSION   DATMCV010000953 DATMCV010000000
VERSION     DATMCV010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317671"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VNU03_21805"
     CDS             complement(<1..1520)
                     /locus_tag="VNU03_21805"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWP77173.1"
                     /translation="MAGFLDVLLRGLILAAQATAVGGVCFVLVALRASATRPGLDAPV
                     LPRSLALIAAGAATLAVGQALALGIELFVLLHDASWPLRAALETLYVQVSAFRVLAGV
                     ALAVCALGLRRRGPTDGLSPRLLAPAILLAAALAVAAPWTSHAAARLESRDVLIVLDG
                     AHQLAAAIWIGGLVHLTAAAVGRRERPWPVGLLRRFSNLAVGAVIGLVAAGVGLTLYY
                     APGITALFGTAYGLMVLTKIVILTGLLALGAFNSRAIRRMDGTSAAADQATMRRFVEV
                     EVGLGLTVLFAAASLTSLPPAVDLTHDRATFAEVATRFTPRLPTLRSPSLAEMPIDDP
                     SAPRNDADRAWSEFNHHVSGMLVLAMGLLAILHTTGRARWARHWPLVFMGLAVFMIFR
                     NDPGSWPLGPEGFWEGLLVATVLQHRIFVLLVIAFAIFEWMVRSGRMRSRRAALVFPL
                     LCAVGGALLLTHSHASQNLKDEFLLEVTHAPLGVMAMLIGWARWLELRLPDARRRVP"
     gene            complement(1522..2682)
                     /locus_tag="VNU03_21810"
     CDS             complement(1522..2682)
                     /locus_tag="VNU03_21810"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWP77174.1"
                     /translation="MTVLAGSALDTPSLLVVLPAPAGAGAPPPETMIAGLPLLRRIVL
                     AAARAGFQRVLVHPRACPEPRLLEGTGASMLRADSVLGAPGRLVLLPVNVLPQARWLR
                     LLREMPLDPGTPAVDPSGTATMEGAEGGVLLRSAGDGALDLIAELGRRPRVVSAGDPA
                     GRFPLVGADGTKAAEAWLLQSLIKDTEGFMSRHVERRISLAITRRLVATSITPNAMTL
                     VSLAVGLLGAPFFLSADPLLQVTGSLLFLTHSILDGCDGELARLKFLESPGGARLDFW
                     GDNLVHVAVFASMAIGWSMATDATWPLLLGALAVTGTAAAAVLVSRRSAAAPTASTAR
                     LAEAVANRDFIYLVVLLACFGRAHWFLIPVALGTPIFMLLVRWGARRRRQAA"
     gene            complement(2679..3746)
                     /locus_tag="VNU03_21815"
     CDS             complement(2679..3746)
                     /locus_tag="VNU03_21815"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWP77175.1"
                     /translation="MGETRRRRGALIGLGNVAVHGHLPGWQAREDCEIVAATDASPAR
                     RAECRARLPAARWHDSVESLLAGEALDFVDICTPPSSHAALIEAALSRGVHVLCEKPL
                     VGAPDELARLAGLAGSAGAVLHTVHNWHHAPLVRATDAVIRAGRIGQVREVVWQTLRT
                     QPAAAAAGDGWRVDPAVAGGGVLTDHGWHVSYVIPRWVGEAPVAVSARLETRRHHRFA
                     VEDTATVRLHFGQAVADVVLTWAADSRENRARVIGTAGRIDIEDDTLVVTAEGREERR
                     PCPPALSDGSQHPDWFTAVADEFLARVEARADRGRSPNLEEAAVCVAIECLARESSRR
                     GGERLALRGLPSSPARAGAAG"
     gene            complement(3733..5061)
                     /locus_tag="VNU03_21820"
     CDS             complement(3733..5061)
                     /locus_tag="VNU03_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWP77176.1"
                     /translation="MTVPREPGPRSREIVAREGRHIAPGYQSFALYSGLAMARGSGSR
                     LWDEDGNEYIDFIAGIAVGSIGHCHPHYVEALKRQAERLTFGSFTTETRARFLELMAK
                     VTPEGLTRIQLFSGGAEAVEAGLRLAKAATGRQEVMSFWGGFHGKTGGVLGLLGSDFK
                     HHQGPFLPGQYSTPYADCYRCPLKLRYPDCGIACAEYVRDAIRYQTAGEIAAIIVEPI
                     QGTAGNVVPPDGFLRAIQSVARDHGALLFADEMITGFGRTGSMWGTDHEGVVPDIMTV
                     GKGVGGGFPLAGVVSTDKLTAAKPWSNPSFSSSSYGGNPLASAAGLAALEVILNEDLV
                     KNADRIGAIMLTELERLKEKYRTVGEVRGRGLLLGIELVEDRQSRRPIGKDITRDLYQ
                     ECLRRGLVAMTYSPTIRINPPLVITEDLAREGLAILDEALGVVLQRHGRD"
     gene            complement(5058..5906)
                     /locus_tag="VNU03_21825"
     CDS             complement(5058..5906)
                     /locus_tag="VNU03_21825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77177.1"
                     /translation="MSERTYWGLFREREHSPGREGDDAEILRLTGKHLEGLGVQVELR
                     APEELSAAETPPDGIFLMCERMEALRVLLAWQERGVRQVNAPGAVLNTYRERMIAQLR
                     EANVPFIQSQVVATQGAEAGLRAPIWVKRPDVHNTQEGDVVFARTRDEAAAALAGLAA
                     RGFERALLQPPVEGDLIKFYGVGAGGGADGGPAWFRWFYHKDQRVAGHPFEPGRLARL
                     VRAAATALGLEIYGGDAIASADGGLVLLDVNAWPSFALYRDEAAPVIAAYLALRFAPR
                     VAGAGQ"
     gene            complement(5926..>6281)
                     /locus_tag="VNU03_21830"
     CDS             complement(5926..>6281)
                     /locus_tag="VNU03_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77178.1"
                     /translation="ARFAARAAARPDEATVLAVTPLVDDEKPLWVTLGAGGRVDAIGV
                     PGGGLVTAGVYRVSARARRVAAAPPLGRLREYLEWLHRSGEPLYGEIIETVVDVDRGL
                     DVAAAEALARGQRRA"
BASE COUNT          897 a         2350 c         2325 g          709 t
ORIGIN      
        1 ggcacgcgtc gccgcgcgtc gggcagccgc agctcgagcc agcgcgccca gccgatcagc
       61 atcgccatca cccccagcgg cgcgtgggtc acctcgagga ggaactcgtc cttcaggttc
      121 tgggaggcgt gggaatgggt caggagcagc gcgccgccga ccgcgcacag gagggggaag
      181 accagcgcgg cccgccgcga ccgcatgcgg ccggagcgga ccatccactc gaagatcgcg
      241 aaggcgatca cgagcagcac gaagatgcgg tgctgcagca ccgtggcgac caggaggccc
      301 tcccagaagc cctcggggcc gagcggccac gaccccgggt cattccggaa gatcatgaag
      361 acggccagcc ccatgaagac gagcggccag tgccgggccc agcgcgcccg gccggtggtg
      421 tgcaggattg cgagcaggcc catggccagg accagcatgc cggagacgtg gtggttgaac
      481 tcggaccagg cgcgatcggc gtcgttgcgg ggagcgctcg gatcgtcgat cggcatctcg
      541 gcgagcgacg gcgagcgcag cgtcggcaga cgcggggtga agcgggtggc gacctcggcg
      601 aaggtggccc ggtcgtgggt gagatccacc gcggggggca gcgaggtcag cgaggcggcc
      661 gcgaagagca cggtgagccc gaggccgacc tcgacctcga cgaagcgtcg catcgtcgcc
      721 tgatcggcgg cggcggacgt tccgtccatc cggcggatcg cccgcgagtt gaacgccccg
      781 agcgcgagga gcccggtcag gatcacgatc ttggtcagga ccatgagccc gtaggcggtg
      841 ccgaagaggg cggtgatccc cggggcgtag tagagcgtga ggcccacccc ggccgccacg
      901 aggccgatga cggctcccac cgcgaggttc gagaatcgcc gcagcagccc gaccggccat
      961 ggccgctccc gccggccgac cgcggccgcg gtcaggtgca cgagcccgcc gatccagatc
     1021 gcggcggcga gctggtgggc gccgtccagc acgatcagga cgtcgcgcga ctcgaggcgc
     1081 gccgccgcgt ggctggtcca cggcgcggcc accgccagtg cggcggccag caggatcgcg
     1141 ggcgccagca ggcgcggcga gagcccatcg gtcgggccgc gacgcctgag cccgagggcg
     1201 cagaccgcga gcgcgacccc ggccagcacg cggaacgcgc tcacctggac gtagagcgtc
     1261 tcgagcgccg cgcgcagcgg ccaggacgcg tcgtgcagga gcacgaacag ctcgatcccg
     1321 agcgcgagcg cctggcccac cgcgagggtg gccgcgccgg ccgcgatcag ggcgagcgag
     1381 cggggcagca cgggagcatc gaggcccggg cgggtggccg acgcccgcag cgcgaccagc
     1441 acgaagcaca ccccgcccac cgcggtagcc tgcgcggcca ggatcagccc gcgcagcagg
     1501 acgtcgagga agccggccac gtcaggcggc ctgacgccgc cgccgcgcgc cccaccgcac
     1561 caggagcatg aagatcggcg tgccgagggc gaccgggatc aggaaccagt gggcccggcc
     1621 gaagcaggcc agcaggacga ccaggtagat gaaatcgcga ttggccaccg cctcggccag
     1681 ccgcgccgtc gacgcggtgg gggcggccgc gctccggcgc gagaccagga ccgcggcggc
     1741 cgcggtgccg gtcaccgcga gggcgcccag cagcagcggc cacgtcgcgt cggtggccat
     1801 gctccagccg atcgccatgg aggcgaacac cgccacgtgg acgaggttgt cgccccagaa
     1861 gtcgagccgg gctccgcccg gcgactcgag gaacttgagc cgggccagct cgccgtcgca
     1921 cccgtcgagg atcgagtggg tcaggaacaa caggctgccg gtcacctgca ggagcggatc
     1981 cgccgagagg aagaagggcg cgcccagcag gcccaccgcg aggctcacca gggtcatcgc
     2041 gttcggcgtg atcgaggtcg caaccaggcg ccgggtgatc gcgagcgaga tccggcgctc
     2101 gacgtggcgg gacatgaacc cctcggtgtc cttgatcagg ctctgcagca gccaggcctc
     2161 cgcggccttg gtcccgtcgg cgcccacgag cggaaaccgg ccggccggat cgcccgcgga
     2221 gacgacccgc gggcggcggc ccagctccgc gatcaggtcc agcgccccgt cgccggccga
     2281 gcgaagcagc acgccgccct ccgcgccttc catcgtagcg gtccccgagg ggtcgacggc
     2341 gggcgtgccg ggatcgagcg gcatctcgcg caggaggcgc agccaccgcg cctggggcag
     2401 cacgttgacc gggaggagga ccagccgccc cggagccccg aggaccgaat cggcgcgcag
     2461 catggaggcg ccggtgccct cgagcagccg gggctcgggg cacgcgcgcg gatgcacgag
     2521 cacgcgctgg aagccggcgc gcgccgcggc cagcacgatc cggcggagca gcggcagccc
     2581 cgcgatcatg gtctcgggcg gaggcgcccc cgcacccgcc ggagcgggca ggaccaccag
     2641 caggctcggc gtgtcgagcg cggacccggc caggaccgtc atcccgcggc cccggcgcgc
     2701 gccgggctcg acgggagccc gcggagcgcc agtcgctcgc ccccgcggcg gctcgattca
     2761 cgcgcgaggc actcgatcgc cacgcagacc gcggcctcct cgaggttggg gctcctgccc
     2821 cggtccgcgc gcgcctcgac gcgcgcgagg aactcgtcgg cgaccgcggt gaaccagtcg
     2881 gggtgctgcg agccgtcgga gagcgcgggc gggcagggcc gccgctcctc ccggccctcg
     2941 gcggtgacca ccagcgtgtc gtcctcgatg tcgatgcggc cggcggtgcc gatcacccgg
     3001 gcccggttct cgcgcgagtc ggcggcccag gtgagcacga cgtccgcgac cgcctgcccg
     3061 aagtggagcc ggaccgtcgc ggtgtcttcc accgcgaagc gatggtgccg ccgcgtctcg
     3121 agccgcgcgc tcaccgccac cggggcctcg cccacccagc gcggaatcac gtaggagacg
     3181 tgccagccgt gatcggtcag caccccgcca cccgcgacgg cgggatccac ccgccagccg
     3241 tcgcccgccg cggccgccgc gggctgggtg cggagggtct gccagacgac ctcgcgcacc
     3301 tggccgatgc ggccggcgcg gatcaccgcg tcggtcgcgc gcaccagcgg cgcgtggtgc
     3361 cagttgtgga cggtgtggag caccgcgccg gccgagccgg cgagcccggc caggcgcgcg
     3421 agctcgtccg gcgcgcccac cagcggcttc tcgcacagga cgtgcacgcc ccgcgagagc
     3481 gcggcctcga tcagggccgc gtggctcgac gggggggtgc agatgtcgac gaaatcgagt
     3541 gcctcgccgg ccagcagcga ctcgaccgag tcgtgccagc gcgcggccgg gagccgcgcc
     3601 cggcactccg cccgccgcgc cgggctcgcg tcggtcgcgg ccacgatctc gcagtcctcc
     3661 cgcgcctgcc agccgggcag gtggccgtgc accgccacgt tgccgaggcc gatgagggcg
     3721 ccgcgccggc gcctagtctc gcccatgccg ctgcagcacc acgccgaggg cctcgtccag
     3781 gatggcgagg ccctcgcgcg cgaggtcctc ggtgatgacg aggggcgggt tgatccggat
     3841 cgtgggcgag taggtcatcg ccacgagccc gcgccgcagg cactcctggt agagatcccg
     3901 ggtgatgtcc ttcccgatcg gccggcggct ctgccggtcc tcgaccagct cgatcccgag
     3961 caggagcccg cgcccgcgca cctcgccgac ggtgcggtac ttctccttga ggcgctcgag
     4021 ctcggtgagc atgatcgcgc cgatccggtc cgcgttcttg accaggtcct cgttcaggat
     4081 gacctcgagc gcggcgagcc ccgccgccga ggcgagcggg ttgcccccgt agctggacga
     4141 gctgaagctc gggttggacc agggcttggc cgcggtcagc ttgtcggtgg agaccacgcc
     4201 ggcgagcgga aagccgccgc ccactccctt gcccaccgtc atgatgtcgg gcaccacgcc
     4261 ctcgtggtcg gtgccccaca tcgagccggt gcggccgaac ccggtgatca tctcgtcggc
     4321 gaagagcagg gccccgtgat cgcgggccac cgactggatc gcgcgcagga agccgtcggg
     4381 agggacgacg ttgcccgcgg tgccctggat cggctccacg atgatcgcgg cgatctcgcc
     4441 cgcagtctgg tagcggatgg cgtcgcgcac gtactcggcg caggcgatgc cgcagtccgg
     4501 atagcgcagc ttgagcgggc agcggtagca gtcggcgtag ggcgtcgagt actggccggg
     4561 caggaacggg ccctggtggt gcttgaagtc gctgccgagc aggcccagca ccccgccagt
     4621 cttgccgtgg aagccgcccc agaaactcat gacctcctgg cgcccggtcg cggccttggc
     4681 caggcggagg ccggcctcca ccgcctccgc gccacccgag aagagctgga tccgggtgag
     4741 gccctcgggc gtcaccttcg ccatcagctc gaggaagcgg gcgcgggtct cggtggtgaa
     4801 gctgccgaag gtcagccgct ccgcctggcg cttgagcgcc tcgacgtagt gcgggtggca
     4861 gtggccgatg ctgcccaccg cgatgcccgc gatgaagtcg atgtactcgt tgccgtcctc
     4921 gtcccacagc cggctgccgc tgccgcgcgc catcgccagc cccgagtaga gcgcgaagga
     4981 ctggtagccg ggcgcgatgt ggcggccctc acgggccacg atctcgcgcg accgggggcc
     5041 gggctcgcgc gggacggtca ttgtccggct cccgccaccc gcggcgcgaa gcgcagggcc
     5101 agatacgccg cgatcaccgg cgcggcctcg tcccggtaga gcgcgaagct cggccacgcg
     5161 ttgacatcga gcagcacgag cccgccgtcg gcggaggcga tcgcgtcgcc accgtagatc
     5221 tcgaggccga gcgcggtcgc ggccgcgcgc accaggcggg ccagccggcc cggctcgaac
     5281 ggatgcccgg ccacccgctg gtccttgtgg tagaaccagc ggaaccaggc cggcccgccg
     5341 tcggcgccgc cgccggctcc caccccgtag aacttgatca ggtctccctc gacaggcggc
     5401 tggagcaacg cgcgctcgaa gccgcgcgcg gccagccccg cgagggcggc cgcggcctcg
     5461 tcgcgcgtgc gcgcgaacac cacgtcgccc tcctgggtgt tgtgcacgtc gggccgcttg
     5521 acccagatcg gggcgcgcag gcccgcctcc gcgccctggg tggccaccac ctggctctgg
     5581 atgaacggca cgttggcctc gcggagctgg gcgatcatgc gctcgcgata cgtgttgagc
     5641 accgcgcccg gcgcgttgac ctgccggacc ccgcgctcct gccacgcgag gagcacgcgg
     5701 agggcctcca tgcgctcgca catgaggaag atgccgtcgg gcggcgtctc ggccgcgctc
     5761 agctcctcgg gcgcccgcag ctcgacctgg acgccgagac cctccaggtg cttgccggtc
     5821 aggcgcagga tctccgcgtc gtcgccctcg cgccccggcg agtgctcccg ctctcgaaag
     5881 aggccccagt aggtccgctc gctcaccccg ctcctcctct caggctcatg cgcgccgttg
     5941 tcctcgcgcg agcgcctccg ccgccgccac gtccaggccg cggtccacgt ccaccaccgt
     6001 ctcgatgatc tcgccgtaga ggggctcgcc gctccggtgc agccactcga ggtactctcg
     6061 cagccgcccg agcggcggcg ccgccgcgac ccgccgcgcc cgcgcggaga cccggtacac
     6121 cccggcggtg accagtccac cgcccggcac gccgatcgcg tccactcgcc cgcccgcgcc
     6181 gagggtcacc cagagcggct tctcgtcgtc gacgagcggg gtcaccgcca gcacggtcgc
     6241 ctcgtccggc cgcgcggcgg cccgagcggc gaaccgcgcg a
//
LOCUS       DATMCV010000954         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_391049, whole genome shotgun sequence.
ACCESSION   DATMCV010000954 DATMCV010000000
VERSION     DATMCV010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391049"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..881)
                     /locus_tag="VNU03_21835"
     CDS             complement(45..881)
                     /locus_tag="VNU03_21835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP77179.1"
                     /translation="MDHGVVMFPTEYSIAPDEFARALEERGFESVWFPEHTHIPASRK
                     SAWPGGGELPREYWSAYDPFVALTAAAGATRRLKLGTGICLVIERDPIVTAKEIATLD
                     RLSGGRVLFGIGGGWNAEEMENHGTAWKSRWRLLRERVLAMKAIWTQKEASYQGEFVR
                     FDRIWSEPKPLQKPHPPIIIGGDGEKTFDRVVEFGDGWMPILRPHTNPVGKIPALHER
                     LRKAGRDPKSAPVSIFFAPPKREVLDGLAAAGVSRAIFGLAPEPRDKILPRLDALAAV
                     MR"
     gene            919..2016
                     /locus_tag="VNU03_21840"
     CDS             919..2016
                     /locus_tag="VNU03_21840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP77180.1"
                     /translation="MASSRHVIVCGAGVVGASVAYFLARRGVRVTVIERTGVACAASG
                     KSGGFLALDWCDNTVVEGLARASFALHAELAHELATDYGYRRMDTFMVAARETGRVSG
                     GHRVAAPSWIDGSAVVAGAIGTTETTAQVHPARFTEALLEGAKGHGATLRLGVVEAVA
                     VREGTARGVVVDGATLEADAVVLAMGPWTGRVGGVRLPPIHGLKGYSVTLAAPDVPAH
                     ALFMDYRTADGDALEPEIIPRPDGTVYVCGMADRQPLPESADGVEVSEAGCAVLARAA
                     GRVSAALATATITRRQACYRPVTDDGVPLIGAVPGTRGAYVATGHGPWGMLNAPATGL
                     ALAELITDGAARAVDLEPFAPARLAATRLRS"
BASE COUNT          254 a          791 c          744 g          296 t
ORIGIN      
        1 gccgtacgcc tcgaagaact tctcgtcgtc gtagacgttc tgcgtcaccg catcacggcc
       61 gcgagggcgt cgagacgcgg caggatcttg tcacggggct cgggcgcgag gccgaagatc
      121 gcgcgcgaca ccccggccgc cgcgagtccg tcgagcacct cccgcttggg cggcgcgaag
      181 aagatcgaca ccggcgcgct cttcgggtcg cggccggcct tacggaggcg ctcgtgcagg
      241 gccgggatct tccccaccgg gttggtgtgc gggcgcagga tcggcatcca gccgtcgccg
      301 aactccacca cccggtcgaa ggtcttctcc ccgtcgccgc cgatgatgat cggcgggtgc
      361 ggcttctgca gcggcttcgg ctcggaccag atccggtcga accgcacgaa ttctccctgg
      421 tagctcgcct ccttctgggt ccagatcgcc ttcatggcca gcacccgctc gcgcagcagc
      481 cgccaccggc tcttccacgc ggtgccgtgg ttctccatct cctccgcgtt ccagccgccg
      541 ccgatgccga agagcacgcg cccgcccgac aggcggtcga gcgtggcgat ctccttggcg
      601 gtgacgatcg ggtcgcgctc gatcaccagg cagatgccgg tgccgagctt gaggcgcctg
      661 gtcgcccccg ccgccgccgt cagggccacg aagggatcgt aggcggacca gtactcgcgc
      721 ggcagctcgc cgccgcccgg ccacgcgctc ttgcggctcg ccgggatgtg ggtgtgctcg
      781 gggaaccaca ccgactcgaa gccgcgctcc tcgagcgcgc gggcgaattc gtcgggagcg
      841 atcgagtatt cggtcgggaa catgacgacg ccgtggtcca tggctccgaa ttgtactacc
      901 ctgtgcggcg aaccggccat ggcctcttcc cggcacgtca tcgtctgcgg cgcgggcgtc
      961 gtgggcgcct cggtcgccta cttcctggcc cgccgcggcg tccgggtcac cgtgatcgag
     1021 cgcaccgggg tcgcgtgcgc ggcgtccggc aagtccggcg ggttcctcgc gctcgactgg
     1081 tgcgacaaca cggtggtcga gggcctcgcc cgcgcgagct tcgcgctcca cgcagagctg
     1141 gcccacgagc tcgccaccga ctacggctac cggcgcatgg acaccttcat ggtggccgcg
     1201 cgcgagacgg gccgggtctc gggcggtcac cgcgtggccg cgccgtcctg gatcgacggc
     1261 tcggcggtcg tggccggcgc gatcggcacc accgagacca ccgcccaggt ccatcccgcg
     1321 cgcttcaccg aggcgctgct cgagggcgcc aagggccacg gcgcgaccct gcgcctgggc
     1381 gtggtggaag cggtcgcggt gcgcgagggc accgcgcgcg gggtggtggt cgacggcgcg
     1441 acgctcgagg ccgacgcggt cgtgctcgcg atgggcccgt ggaccgggcg cgtcgggggc
     1501 gtgcgcctgc cccccatcca cgggctcaag ggctacagcg tgacgctggc cgcgcccgac
     1561 gtgcccgccc acgccctctt catggactac cgcaccgccg acggcgacgc gctcgagccc
     1621 gagatcatcc cgcggcccga cgggaccgtg tacgtctgcg gcatggccga tcgccagccg
     1681 ctgccggagt ccgccgacgg cgtggaggtg agcgaggcgg gctgcgcggt cctcgcgcgc
     1741 gcggccggcc gcgtctccgc cgcgctggcc accgcgacca tcacccgccg gcaggcctgc
     1801 taccggccgg tcaccgacga cggcgtgccg ctgatcggcg cggtgccggg cacgcggggc
     1861 gcctacgtcg cgaccggcca cggcccctgg ggtatgctca acgcgccggc caccgggctg
     1921 gccctggccg agctgatcac ggacggcgcc gcccgggcgg tcgacctcga gcccttcgct
     1981 ccggctcgac tggccgcgac ccggctgcgc tcctaaccga ccgaacggac cggaggcgtc
     2041 atgcgacgac tcgccctcac cgttctgctg ctccggctcg cgctc
//
LOCUS       DATMCV010000955         4997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_118617, whole genome shotgun sequence.
ACCESSION   DATMCV010000955 DATMCV010000000
VERSION     DATMCV010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118617"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..941)
                     /gene="cdaA"
                     /locus_tag="VNU03_21845"
     CDS             complement(117..941)
                     /gene="cdaA"
                     /locus_tag="VNU03_21845"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HWP77181.1"
                     /translation="MWPVLQTFRPRDAIDIVLVAIVLYRVFVMFKETRAVQMLLGLAG
                     LMVASFLARHFELFSTSWLLDNFWSFWVLALIVLFQPELRRALTRLGESRLFQGMSLG
                     AREERSHLIDEVIKAADALASKRIGALIVLERSTGLRNYAELGVPLDAVVSADLLVSL
                     FLPYSPLHDGAAFIRGDRVAAAGCFLPLSRNTQLGRNMGTRHRAGLGLAEETDAVVLI
                     VSEESGHTSLAVDGQMESPLDQESLRRRLGDLFSLEEAPMPRRAAWWEPAREWLRK"
     gene            complement(944..1798)
                     /gene="folP"
                     /locus_tag="VNU03_21850"
     CDS             complement(944..1798)
                     /gene="folP"
                     /locus_tag="VNU03_21850"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014788181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWP77182.1"
                     /translation="MNRATVAGVSVGDGLDVAIVGALNVSPESFYSGSVVTDADRLLQ
                     AAEAMVQAGAAWLDVGAMSTAPYLEALIPETQEADRLHWAVGLLTAKLDLPVCADTSR
                     ALPARAALESGARMLNDVTGLAPDPALARLAAEAGAGLVLMAAPAAAPAPGEPLETVL
                     ASLTGSLARARAAGMPDDRILVDPGIGFFRGGRIEWPEWDCRVLAGLPVLRSLGRPIY
                     VGVSRKSFIGALAGAPDPADRLPGTLAATAAAVQGGAHVIRAHDVAETAQAVRVAQAI
                     HRAGTGRA"
     gene            complement(1869..3680)
                     /gene="ftsH"
                     /locus_tag="VNU03_21855"
     CDS             complement(1869..3680)
                     /gene="ftsH"
                     /locus_tag="VNU03_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWP77183.1"
                     /translation="MNPFYKNLALWMVIGLIVILLFQLFQQNQSPRGEIVFSDFLKKV
                     ESGEVREVTLKGNNVSGRMSDGSSFRTFTADYPDLVKSLKDKGVKIDVKPPDTNSWLA
                     IVLQWVPMLLFIGVWIFFMRQMQGGGAKALSFGKARARLISEKQNKVTFQDVAGVDEA
                     KEELREIIEFLKDPPKFQKLGGKIPKGVLLVGPPGTGKTLLAKAIAGEANVPFFSISG
                     SDFVEMFVGVGASRVRDLFEQGKKHAPCIIFMDEIDAVGRHRGAGLGGGHDEREQTLN
                     QLLVEMDGFETNEGVILIAATNRPDVLDPALLRPGRFDRQVVVARPDVKGREEILKVH
                     SRRIPLAPKVDLKVLARGTPGFSGADLANLVNEAALLAARQDKKVVEMIDFENAKDKV
                     LMGVERRSMIISETEKKTIAYHEAGHALVADLLPGADPLHKVTIIPRGRALGLTQQLP
                     ADDKYNYSRDYLVNRITILLGGRAAEEIVFQQQTTGAGDDLEKATEMARKMVCEWGMS
                     DKMGPLTFGKGEEHIFLGREVARPKDFSEETAVLIDSEIKRIVIECATRARHMIETNL
                     EKLHALARALLERESLDGEEIARILRVPPLHEAPSPA"
     gene            complement(3736..4272)
                     /gene="hpt"
                     /locus_tag="VNU03_21860"
     CDS             complement(3736..4272)
                     /gene="hpt"
                     /locus_tag="VNU03_21860"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWP77184.1"
                     /translation="MDRQLGRILISRDEIAARVAELGEAIGRDYAGRAPLLVGVLKGA
                     VVFTSDLLRAIPLPAAMDFMAVSSYGSGTRSSGVVRLTADLSLSIEGRDVIIVEDVID
                     SGRTISYLRRNLATRHPRSLALCVLLDKVSRREVDVEVDYVGFVVPDEFVVGYGLDYD
                     GWHRNLPDLAVLESATRA"
     gene            complement(4285..>4997)
                     /locus_tag="VNU03_21865"
     CDS             complement(4285..>4997)
                     /locus_tag="VNU03_21865"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWP77185.1"
                     /translation="ARRRAGGGPGFGQWLVLGGAVVVILGLTFALGLLVGRQLARSSL
                     AAAPPSASEAARKTGAPAPRRGGIAAETMADRPPEPTEKLTFYQTLTEPLNGGGPARP
                     EAKPVTVKVSAPSPAPPAAAVPAPTPASPPVSLPPAAGKVPVPTAPAAKPAASPAAGA
                     PWTVQVGAFKNRRQAEDTRQQLATAGLEAYVASVAAQDGQPRFRVRVGTYRSREEAAA
                     VAERIRAQRSLTAFATPK"
BASE COUNT          733 a         1774 c         1797 g          693 t
ORIGIN      
        1 ctcgatcggc gccgcgacca ccacgtcggc cttctcggag gtcatgacga agacccagag
       61 caccattgag accaccaggg cgagcagctt cagctcccag tgccgggtga gcgcgctcac
      121 ttgcgcagcc actcccgcgc cggctcccac cacgcggcgc gccggggcat gggggcctcc
      181 tccagggaga agagatcgcc cagtcggcgg cgcagcgact cctggtcgag cggcgactcc
      241 atctgcccgt cgaccgcgag cgaggtgtgc ccgctctcct cggagacgat cagcaccacc
      301 gcgtcggtct cctcggccag cccgagcccc gcgcgatgcc gcgtgcccat gttccggccg
      361 agctgcgtgt tgcgcgagag cggcaggaag cagcccgccg cggccacgcg gtcgccgcgg
      421 atgaaggcgg cgccgtcgtg cagcggcgag tagggcagga agaggctcac gagcaggtcg
      481 gcggagacga ccgcgtcgag cgggacgccg agctccgcgt agttgcgcag gccggtggac
      541 cgctccagca cgatgagggc gccgatgcgc ttgctcgcga gcgcgtccgc ggccttgatc
      601 acctcgtcga tgaggtggct ccgctcctcg cgcgcgccca ggctcatgcc ctggaagaga
      661 cggctctcgc ccaggcgcgt gagggcccgc cgcagctcgg gctggaagag gacgatcagg
      721 gccagcaccc agaacgacca gaagttgtcg agcagccagc tcgtgctgaa cagctcgaag
      781 tggcgggcca ggaacgaggc caccatgaga ccggcgaggc ccaggagcat ctgcaccgcg
      841 cgcgtctcct tgaacatcac gaagacgcgg tagagcacga tcgccaccag cacgatgtcg
      901 atcgcgtcgc gcggacggaa ggtctggagc acgggccaca tggctaggcg cgccccgtcc
      961 cggcccggtg gatcgcctgg gcgacccgga ccgcctgcgc ggtctcggcc acgtcgtggg
     1021 cccggatcac gtgggcccca ccctgcaccg cggccgcggt ggcggccagc gtgccgggca
     1081 gccggtcggc cggatcgggc gcgccggcca gcgcgccgat gaaggacttc cgggacacgc
     1141 cgacgtagat ggggcgcccg aggctccgga ggacgggcaa gccggccagc acccggcagt
     1201 cccactcggg ccactcgatg cggccgccgc ggaagaatcc gataccgggg tcgacgagga
     1261 tccggtcgtc gggcatcccc gccgcccgcg cccgggcgag gcttccggtc agggacgcca
     1321 gcaccgtctc gaggggctca ccgggagccg gggcggccgc aggagcggcc atgagcacga
     1381 gcccggcgcc ggcctcggcg gccaggcggg ccagcgcggg atccggagca agcccggtca
     1441 cgtcgttgag catgcgggcg ccgctctcga gagcggcccg ggcgggcagc gcccggctcg
     1501 tgtcggcgca gaccggcagg tcgagcttgg cggtcagcag gcccaccgcc cagtgcaggc
     1561 gatcggcctc ctgcgtctcc gggatgagcg cctcgaggta gggcgcggtg gacatcgccc
     1621 ccacgtcgag ccaggccgcg cccgcctgga ccatcgcctc ggcggcctgg aggagccgat
     1681 cggcgtcggt gacgacggag cccgagtaga acgactccgg gctcacgttg agggctccca
     1741 cgatggccac gtccaggcca tcgcccaccg acactcctgc cacggtcgcg cgattcacgg
     1801 ggagagctcc acgatcaact tatcaccgca atgggccccg gcgggccccg aatttgcggg
     1861 cgtggaggct acgcggggct gggcgcctcg tggagcgggg gcacccggag gatccgggcg
     1921 atctcctcgc cgtccagcga ctcgcgctcg aggagggcac gggcgagcgc gtggagcttc
     1981 tcgaggttgg tctcgatcat gtgacgggcg cgcgtggcgc actcgatcac gatccgcttg
     2041 atctcggagt cgatcaggac cgcggtctcc tcgctgaagt ccttcgggcg ggccacctcg
     2101 cggccgagga agatgtgctc ctcgcccttg ccgaaggtga gcgggcccat cttgtcggac
     2161 atgccccact cgcagaccat cttgcgggcc atctcggtgg ccttctcgag atcgtccccc
     2221 gccccggtgg tctgctgctg gaagacgatc tcctccgccg cgcggccgcc gaggaggatc
     2281 gtgatccggt tcacgaggta gtccctcgag tagttgtact tgtcgtcggc gggcagctgc
     2341 tgggtgaggc cgagggcccg gccgcggggg atgatcgtga ccttgtgcag cgggtccgcc
     2401 cccggcagca ggtccgccac cagcgcgtgg cccgcctcgt ggtacgcgat ggtcttcttc
     2461 tcggtctccg agatgatcat cgagcgccgc tccacgccca tcaggacctt gtccttggcg
     2521 ttctcgaagt cgatcatctc gacgaccttc ttgtcctggc gcgcggccag gagcgcggcc
     2581 tcgttcacca ggttggccag gtccgccccc gagaaaccgg gcgtgccgcg ggccagcacc
     2641 ttgaggtcga ccttgggcgc gagcgggatg cgccgcgagt ggaccttgag gatctcctcg
     2701 cgccccttca cgtcgggccg cgccaccacc acctggcggt cgaagcggcc gggccgcagc
     2761 agcgccgggt ccagcacgtc cggccggttg gtggcggcga tcaggatcac gccctcgttg
     2821 gtctcgaagc cgtccatctc gaccagcagc tggttcagcg tctgctcgcg ctcgtcgtgg
     2881 ccgccgccca ggcccgcgcc ccggtgccgg ccgaccgcgt cgatctcgtc catgaagatg
     2941 atgcagggcg cgtgcttctt gccctgctcg aagaggtcgc gcacccggga ggcgcccacg
     3001 ccgacgaaca tctcgacgaa gtcggagccg gagatggaga agaacggcac gttggcctcg
     3061 cccgcgatgg ccttggccag gagcgtcttg ccggtgcccg gagggccgac cagcagcacg
     3121 cccttcggaa tcttgccgcc gagcttctgg aacttgggcg gatccttcag gaactcgatg
     3181 atctcgcgca gttcttcctt ggcctcgtcg acgccggcca cgtcctggaa ggtgaccttg
     3241 ttctgcttct ccgagatcag ccgggcccgc gccttgccga aggacagggc cttggcgccc
     3301 ccgccctgca tctgccgcat gaagaagatc cagaccccga tgaacagcag catcggcacc
     3361 cactgcagca cgatggccag ccacgagttg gtgtcgggcg gcttgacgtc gatcttgacg
     3421 cccttgtcct tgagggactt gaccaggtcc gggtagtcgg cggtgaaggt ccggaacgac
     3481 gagccgtcgc tcatgcgccc ggagacgttg ttgcccttga gggtgacctc gcgcacctcc
     3541 cccgactcga ccttcttgag gaagtcggag aagacgatct cgccccgcgg cgactgattc
     3601 tgctggaaga gctggaagag gaggatgacg atgaggccga tcaccatcca cagggcgagg
     3661 ttcttataga acggattcat acggggaatc ataccatacg gggcgacgcg cggcggggcc
     3721 gcccgcgagc gcggatcagg ctctcgtggc cgactccagg acggcgagat cgggcaggtt
     3781 acggtgccag ccgtcgtagt cgagaccgta gcccaccacg aactcgtcgg gcacgacgaa
     3841 gcccacgtag tcgacctcga cgtcgacctc ccggcgggac accttgtcca gcagcacgca
     3901 cagggcgagg ctccgcggat gccgggtcgc caggttccgg cgcaggtagc tgatggtgcg
     3961 cccggagtcg atgacgtcct ccacgatgat cacgtcccgc ccctcgatcg acagcgacag
     4021 gtcggcggtg agccgcacga ccccggagga ccgggtgccc gagccgtagg acgagaccgc
     4081 catgaagtcc atcgcggcgg gcagcgggat ggcccggagc aggtcggagg tgaacaccac
     4141 cgcgcccttc aggaccccga ccagcagcgg agctcggccc gcgtagtccc ggccgatggc
     4201 ttccccgagc tccgcgacgc gggccgcgat ctcgtcccgg gagatgagga tgcggccgag
     4261 ctgccggtcc accgcggggg cgccttactt gggcgtggcg aaggcggtga gcgaccgctg
     4321 ggcgcggatc cgctcggcga ccgccgcggc ttcctcgcga ctccggtacg tgccgacccg
     4381 gacccggaag cgcggctgac cgtcctgggc ggccacgctg gccacgtagg cctcgagccc
     4441 ggccgtggcc agctgctggc gggtgtcctc ggcctggcgc cgattcttga aggcaccgac
     4501 ctggaccgtc cacggcgccc cggcggcggg gctcgcagcg ggcttcgccg cgggggccgt
     4561 gggcactggg accttgcctg cagcgggcgg cagcgagacg ggcggggacg ccggcgtcgg
     4621 cgcgggcacc gccgcggcgg gcggagccgg gctcggcgcc gacaccttga ccgtgaccgg
     4681 cttcgcctcc ggtctcgccg gcccgccgcc gttgagaggc tcggtcagcg tctgatagaa
     4741 cgtcagcttc tcggtcggct cgggcggccg gtcggccatg gtctccgccg cgatgcctcc
     4801 gcggcgcggc gcgggcgcgc cggtctttcg cgccgcctcg gaggcggacg ggggcgcggc
     4861 ggcgaggctg ctgcgcgcga gctggcggcc caccagcagg cccagggcga aggtcagtcc
     4921 gaggatcacc accaccgccc cgcccagcac gagccactgg ccgaaacccg gtccaccgcc
     4981 cgcccgccgt cgcgccg
//
LOCUS       DATMCV010000956         7784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_785477, whole genome shotgun sequence.
ACCESSION   DATMCV010000956 DATMCV010000000
VERSION     DATMCV010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785477"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VNU03_21870"
     CDS             complement(<1..586)
                     /locus_tag="VNU03_21870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77186.1"
                     /translation="MGALEGKVAIVAGGGTGIGRAVALCFAREGARVAVFGRRPEPLR
                     EVAQAIAAAGGIGHAEAGDVGVEADVARLVSETRRLWGGVDVLVNSAAVRLNAPLEKV
                     SADDFAEVLRINLVGAFVLTKMVAGPMRERGGGSIVHISSALGEVAVRDFSPYVASKG
                     GLNALARAAAIELVGDRIRVNVVAPGTIDTAINPG"
     gene            complement(567..1268)
                     /locus_tag="VNU03_21875"
     CDS             complement(567..1268)
                     /locus_tag="VNU03_21875"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWP77187.1"
                     /translation="MTSLPDRRTRVRFRRRLLAWYGRHRRDLPWRHTEDPYHILVSEI
                     MLQQTQVDRVVPKYHEFLDRYPTLEALAASAPNDVKQLWYPLGYNVRPLNLHGIACEA
                     VATYGGRLPDDDDALRAMRGIGRYTAGAVRCFAYGRPVPIVDTNVRRVLGRVFLGPRR
                     LARLRGQKAMWDLAGALVPPRKAYDYNQALMDFGATWCTPRAPRCRPCPMRRSCATYR
                     AERRGRGGADGRARG"
     gene            1347..2333
                     /locus_tag="VNU03_21880"
     CDS             1347..2333
                     /locus_tag="VNU03_21880"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWP77188.1"
                     /translation="MPDQTREYDAARHGVALFDRSSLGKVSVTGRDRLAFLQGMLTND
                     VKALAPGQGAPAAFLDAHGKVTVLLVVYAAQDRVLIELPAQMTAKTLETLDHFLISEK
                     AEFEAVDEAVAILSLQGRGARALLEGLAGATLDLAPYAHAEVTVGGAPVRVINRAEGP
                     APGFHCWTEAAGAESLRSAAIAAGAVVAGPATLEILRIEAGQPWYPQDVDQSVILPET
                     RLEALVSYTKGCYIGQETVARVKYRGHVNRALSGLLVEGDRVPEVGARVVVEGAEVGK
                     VTSAARSIALGRPIALGYVRREHFEPGTAVTIADGGGEQPARVSALPFVSET"
     gene            2348..4420
                     /locus_tag="VNU03_21885"
     CDS             2348..4420
                     /locus_tag="VNU03_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWP77189.1"
                     /translation="MSQSRHANRLASETSPYLLQHAHNPVDWYPWSPEAFELARREDK
                     PVLLSVGYSACHWCHVMERESFENDDIAALMNQLFVSIKVDREERPDVDQIYMQAVQS
                     MTGRGGWPMTVFLTPDGVPFYGGTYFPPEDRHGMPAFPRLLRSIADAYHSRRSEVLEA
                     GRQLVETMGQSGRLTGSTSLLGRDVLSRAYLGISGEFDERDGGLGQAPKFPQPMTWEA
                     VLRVARRTGDARGLGMLRHTLTRMARGGIYDQLGGGFHRYSVDGQWLVPHFEKMLYDN
                     AQLASLYLHGWLATGEPLYRRIAEETLDYVRREMTHPAGGFYSAQDADSEGVEGKFFV
                     WSADEIRAVLGDPALARAALAYWGVDDGPNFEGHSILFVPREPEEVAGTLGMTVDELM
                     AAIGRARALLRAAREPRVHPALDDKVLASWNGLMLAAFAEAASVLGRADYLAAAVRNA
                     EFLGRQMVRDGRLLRSWKDGQARITGYLEDYSMVGAGLLALYEATFDRRWLDESRRLA
                     EEALRLFWSADRNAFFDTGDDQEALVVRPRNLFDNAVPSGTSVAIDWLLRLAVMVGEE
                     RYQAIALEALRPMADLMQRYPSGFGRYLSALDFHLGPVAEVALVWRPGEERAMGPLLD
                     TVFGRYQPNRVVVGAPEGDAAAAGLPLLAERATVGGRPTAYVCQHYVCQLPVTEPDAL
                     ARQLDAGV"
     regulatory      4427..4527
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4568..4768
                     /gene="thiS"
                     /locus_tag="VNU03_21890"
     CDS             4568..4768
                     /gene="thiS"
                     /locus_tag="VNU03_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWP77190.1"
                     /translation="MTITVNGKPMELPAGLTIEGLLAHLKVRREFTAVALNREVALRA
                     HYAETQLHDGDKVEIVHPMGGG"
     gene            4851..5633
                     /locus_tag="VNU03_21895"
     CDS             4851..5633
                     /locus_tag="VNU03_21895"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWP77191.1"
                     /translation="MYDTSLWLGGKEFKSRLIVGTGKYPSFENMREAIEASGAEIVTV
                     AVRRVSLPGQGESLLDYVDPKRYTLLPNTAGCYSADEAVRTCYLAREAGLGTLVKLEV
                     IGDSRTLFPDVLGLLEATRTLAREGFTVLPYSNDDPVMAKKLEDAGAAAVMPLGAPIG
                     SGLGIRNPYNIKLILEAVTVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIASAKDPV
                     AMATAMRLAVEAGRLAFKAGRIGKKLYATASSPLEGVPEWTS"
     gene            5621..6247
                     /gene="thiE"
                     /locus_tag="VNU03_21900"
     CDS             5621..6247
                     /gene="thiE"
                     /locus_tag="VNU03_21900"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWP77192.1"
                     /translation="MDQLGFRLYLITDRAASPRPPVEVVEECLDAGLRAVQLREKDLE
                     VRALLALATPLREATRHHGARLLINDRADVALAVGADGVQRTHASLPVAALREITPPG
                     FLIGASAHSTAEAREAAAQGADFVVFGPVYDTPSKRRYGPPQGLAALEAAAAASTRPL
                     LAVGGITPERVGEVRAAGAAGVAVIGAIYGAARPADATKAFLDVLGSA"
     gene            6244..7362
                     /gene="thiO"
                     /locus_tag="VNU03_21905"
     CDS             6244..7362
                     /gene="thiO"
                     /locus_tag="VNU03_21905"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HWP77193.1"
                     /translation="MMTSGPSVLVIGGGIIGCSAAYELAKRGCRVTVLERGTPGGEAS
                     GAAAGLLSPLGDSPDPSPFNRLAIESWRLYPRVVAELREVTGVDVEHMTAGTLYPLAS
                     AAGVEDARARCGWPLAAEFGVEMVEGTALAALEPALAKDVTAALLVRGDHWVNNQRLV
                     TAYALAAAARGVTVRTGVEVGRILVERGRAAGVLADGERLTADAVLLAAGAWSGGLAA
                     DLRGRLPVGPVRGQMLAVSNVPALISHAMHGDDVYLVPRPSGELLIGATIERVGFERA
                     VTPEGLGALIAQAVALVPEIGRRAITRSWYGFRPAAPDGLPVLGPWPDVADLFVATGH
                     YRNGILLAPVTAAVMAACIVDDKTPDSITPFLPQRFVS"
     gene            7372..>7784
                     /locus_tag="VNU03_21910"
     CDS             7372..>7784
                     /locus_tag="VNU03_21910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWP77194.1"
                     /translation="MQTKTGLKQSICEAIDRNAERIIALGERIRRQPELGFKEFKTAR
                     LAEETFSELGLKPRGGLALTGVRAEAAGAKDGPTFALLGELDGLVVAGHPIADPETGA
                     AHACGHNAQMAGLLGAAMGLLEARAFEHLAGRVVF"
BASE COUNT         1053 a         2852 c         2813 g         1066 t
ORIGIN      
        1 ccccggggtt gatggcggtg tcgatggtgc ccggggccac cacgttcacc cggatgcggt
       61 ccccgaccag ctcgatcgcg gcggcccggg ccagcgcgtt gaggccgccc ttggaggcga
      121 cgtagggcga gaagtcgcgc accgccacct cgccgagcgc tgagctgatg tgcacgatgg
      181 atccgccgcc ccgctcgcgc atggggcccg ccaccatctt ggtgagcacg aaggcgccca
      241 ccaggttgat gcgcagcacc tccgcgaagt cgtccgccga caccttctcg agcggagcgt
      301 tgaggcgcac cgccgccgag ttcaccagga cgtccacgcc gccccagagc cgccgcgtct
      361 cggacaccag gcgggccacg tccgcctcca cgcccacgtc gccggcctcg gcgtgaccga
      421 tcccgccggc ggcggcgatg gcctgcgcca cttcccgcag cggctccggc cgccggccga
      481 acacggccac gcgcgcgccc tctcgcgcga agcagagcgc gaccgcccgg ccgatgccgg
      541 tgccgccacc cgccacgatc gcgaccttac cctcgagcgc gcccatcggc tcctccacgc
      601 ccccggcgct ccgcgcggta ggtcgcgcac gagcgccgca tcgggcaggg gcgacagcga
      661 ggcgcccgcg gggtgcacca ggtggcgccg aagtccatca gcgcctggtt gtagtcgtag
      721 gccttgcgcg gcggcaccag cgcgccggcc aggtcccaca tcgccttctg cccgcgcagc
      781 cgcgccaggc gccgcgggcc caggaacacg cggccgagca cccggcgaac gttggtgtcg
      841 acgatcggca ccggccgccc gtaggcgaag cagcgcaccg cgccggcggt gtagcgcccg
      901 atcccgcgca tcgcccgcag tgcgtcgtca tcatcgggca gccgcccgcc gtaggtggcg
      961 accgcctcgc acgcgatgcc gtgcaggttg agcggccgca cgttgtaccc gagcgggtac
     1021 cagagctgct tcacgtcatt gggggcggag gcggcgaggg cctccagggt ggggtagcga
     1081 tcgaggaact cgtggtactt gggcaccacg cgatcgacct gggtctgctg cagcatgatc
     1141 tcggagacca ggatgtggta gggatcctcg gtgtgccgcc acggcaggtc gcgccggtgc
     1201 cggccgtacc aggcgaggag acgccgccgg aagcggaccc gagtccggcg gtcgggcagg
     1261 gaggtcatcg gcacggcggc cattgtgtac gaggttcgcg gcttcccgca agttgacagc
     1321 cgcccgtcgg ccgacaacaa tgagccatgc ccgaccagac ccgcgagtac gacgccgccc
     1381 gccacggagt cgcgctgttc gaccgctcct cgctcggcaa ggtgagcgtg acgggccgtg
     1441 accgcctggc cttcctgcag ggcatgctca ccaacgacgt gaaggccctc gccccggggc
     1501 agggcgcgcc ggccgccttc ctcgacgccc acggcaaggt caccgtcctc ctggtggtat
     1561 acgccgccca ggaccgggtc ctgatcgagc tgccggcgca gatgaccgcg aagaccctcg
     1621 agaccctcga ccatttcctg atctcggaga aggcggagtt cgaggcggtc gacgaggcgg
     1681 tcgcgatcct ctcgctgcag ggccgggggg cgcgcgcgct gctcgaaggc ctcgccgggg
     1741 ccacgctcga tctcgccccg tacgcccacg ccgaggtcac ggtgggcggc gcgccggtgc
     1801 gggtgatcaa ccgcgcggag ggcccggctc ccggcttcca ctgctggacc gaggccgcgg
     1861 gcgcggagtc gctgcggagc gcggccatcg ccgccggcgc cgtcgtcgcg gggccggcca
     1921 ccctcgagat cctgcgcatc gaggccgggc aaccctggta cccccaggac gtcgaccagt
     1981 cggtgatcct ccccgaaacc cggctcgagg cgcttgtgag ctacaccaag ggctgctaca
     2041 tcggccagga gacggtggcg cgcgtgaagt accgcgggca cgtcaaccgg gcgctgagcg
     2101 gcctgctcgt cgagggcgac cgcgtgccgg aggtgggcgc gcgggtcgtg gtggagggcg
     2161 cggaggtggg caaggtgacg tccgcggcgc gctccatcgc gctcggccgg ccgatcgcgc
     2221 tcggctacgt gcgtcgcgag cacttcgagc ccggtaccgc ggtgacgatc gcggacggcg
     2281 gcggcgagca gccggcccgg gtgagcgcgc tgccgttcgt gtccgagacc tgaccggacc
     2341 cgtccgcgtg tcccagtccc gtcacgccaa tcggctcgcc tccgagacga gcccctacct
     2401 gctccagcac gcccacaacc cggtcgactg gtatccctgg agcccggagg cgttcgagct
     2461 cgcccgccgc gaggacaagc cggtgctgct gtcggtcggc tactccgcgt gccactggtg
     2521 ccacgtgatg gagcgcgagt cgttcgagaa cgacgacatc gccgccctca tgaaccagct
     2581 cttcgtctcg atcaaggtag accgcgagga gcggcccgac gtcgaccaga tctacatgca
     2641 ggcggtgcag tcgatgaccg gccggggcgg ctggccgatg accgtcttcc tgaccccgga
     2701 cggcgtcccc ttctacggag ggacgtactt ccctccggag gatcgccacg gcatgcccgc
     2761 cttcccgcgg ctcctgcgct cgatcgcgga cgcctaccac tcccggcgca gcgaggtgct
     2821 cgaggcgggc cgccagctcg tcgagaccat ggggcagtca gggaggctga ccggatcgac
     2881 gagcctcctc ggccgcgacg tgctcagccg ggcctacctc ggcatcagcg gcgagttcga
     2941 cgagcgcgac ggcggcctcg gacaggcgcc caagttcccc cagccgatga cctgggaggc
     3001 ggtgctgcgc gtcgcgcgcc gcaccggcga cgcgcggggc ctcgggatgc tccgccacac
     3061 gctcacccgc atggcccgcg gcggcatcta cgaccagctc ggcggcggct tccaccgcta
     3121 ctcggtggac ggccagtggc tggtgccgca cttcgagaag atgctctacg acaacgccca
     3181 gctggcctcg ctctacctgc acggctggct cgccaccggc gagccgctct accggcgcat
     3241 cgccgaggag accctcgact acgtccggcg cgagatgacc cacccggccg gcggcttcta
     3301 ctcggcccag gacgccgact cggagggcgt cgagggcaag ttcttcgtct ggtcggccga
     3361 cgagatccgc gcggtcctgg gcgatccggc cctggcgcgc gcggcgctcg cctactgggg
     3421 cgtcgacgac gggcccaact tcgagggcca cagcatcctg ttcgtcccgc gcgagccgga
     3481 ggaggtcgcg gggacgctcg gcatgacggt cgacgagctg atggccgcga tcgggcgcgc
     3541 ccgcgcgctc ctgcgcgccg cgcgcgagcc ccgcgtgcac cccgccctcg acgacaaggt
     3601 gctcgcctcg tggaacgggc tcatgctcgc cgcgttcgcg gaggccgcct ccgtgctggg
     3661 ccgcgccgac tacctggcgg ccgcggtccg caacgccgag ttcctcggcc ggcagatggt
     3721 ccgcgacggc cgcctgctcc gctcctggaa ggacggccag gcccgcatca ccggctacct
     3781 cgaggactac tcgatggtgg gggcggggct gctcgccctc tacgaggcca ccttcgaccg
     3841 gcgctggctc gacgagtcgc gacgcctcgc cgaggaggcc ctgcggctct tctggagcgc
     3901 cgatcgaaac gcgttcttcg atacggggga tgaccaggag gccctcgtgg tgcggccgcg
     3961 caacctcttc gacaacgcgg tgccgagcgg cacctcggtg gcgatcgact ggctgctgcg
     4021 gctggccgtg atggtgggcg aggagcgcta ccaggcgatc gcgctcgagg ccctccgccc
     4081 catggcggac ctcatgcagc gctacccgtc gggcttcggc cgctatctct ccgcgctgga
     4141 cttccacctg ggcccggtgg ccgaggtggc gctggtctgg cgtccgggcg aggagcgggc
     4201 gatgggtccc ctcctcgaca ccgtgttcgg ccgctaccag ccgaaccggg tggtggtggg
     4261 tgcgcccgag ggcgatgccg ccgccgcggg gctgccgctg ctggccgagc gggccaccgt
     4321 gggcggccgg cccaccgcct acgtgtgcca gcactacgtc tgccagctcc cggtgacgga
     4381 gccggacgcg ctcgcgcgcc agctcgacgc tggggtataa tccggatcgc gtcgctgggg
     4441 gaggtttcct gagaaccggg tgcggcccgg gacccctcga acctgcactg ggtaattcca
     4501 gcgaagggaa tgcgacgcga ggcatcactg gaccgcaccc ttttctggaa aggtgtgcgg
     4561 ttttttcatg accatcaccg tcaacggcaa gccgatggag ctgcccgcgg gcctgacgat
     4621 cgaaggcctg ctcgcgcatc tcaaggtgcg gcgggagttc accgcggtgg ccctgaaccg
     4681 cgaggtggcc ctccgcgccc actacgccga gacgcagctg cacgacgggg acaaggtcga
     4741 gatcgttcac cctatgggcg gcgggtagtg aacggctgga ttccagggca gttgcttggc
     4801 gaggtgtagc cgaaggcgag ccgagcggat caataatcgg gaggtccata atgtacgaca
     4861 cttcattgtg gctgggtggg aaagagttca agtcgcgtct catcgtcggc acgggaaagt
     4921 acccgtcgtt cgagaacatg cgcgaggcca tcgaggcctc cggagcggag atcgtcacgg
     4981 tcgcggtccg tcgggtgagc ctgcccgggc agggcgagtc gctgctcgac tacgtcgacc
     5041 ccaagcggta cacgctcctg ccgaacaccg ccggctgcta ctcggcggac gaggcggtgc
     5101 gcacctgcta cctggcgcgc gaggccggcc tcggcaccct ggtgaagctc gaggtcatcg
     5161 gcgactcgcg caccctcttc ccggacgtcc tgggcctgct cgaggccacc cgcacgctgg
     5221 cgcgcgaggg cttcacggtg ctgccctaca gcaacgacga cccggtcatg gccaagaagc
     5281 tcgaggacgc gggggctgcc gcggtcatgc cgctgggcgc gccgatcggc tccgggctcg
     5341 gcatccgcaa cccgtacaac atcaagctca tcctggaggc ggtcaccgtc ccggtgatcg
     5401 tggacgccgg ggtcggcacc gcgtccgacg cggccatcgc catggagctc ggctgcgacg
     5461 gcgtgctcat gaacaccgcg atcgcctccg ccaaggaccc ggtcgcgatg gccaccgcca
     5521 tgcgcctggc cgtcgaggcg gggcgcctcg cgttcaaggc cggccgcatc ggcaagaagc
     5581 tgtacgccac cgcgtcctct ccgctggagg gcgtgcccga gtggaccagc taggcttccg
     5641 cctctacctg atcaccgacc gcgccgcgtc gccgcggccg cccgtggagg tcgtcgagga
     5701 gtgtcttgac gccggcctcc gcgcggtgca gctccgcgag aaggacctcg aggtgcgcgc
     5761 gctgctcgcc ctggccaccc cgctgcggga ggccactcga caccacgggg cgcgcctgct
     5821 gatcaacgac cgggccgacg tggcactggc ggtgggcgcc gacggggtgc agcgcaccca
     5881 cgcctcgctg ccggtggccg cgctgcggga gatcaccccg cccgggttcc tcatcggcgc
     5941 ctccgcgcac tcgaccgcgg aggcgcgcga ggcggcggcg cagggggccg acttcgtggt
     6001 gttcggcccg gtttacgaca cgccgtcgaa gcgtcgctac ggtccgcccc agggcctggc
     6061 cgccctcgag gcggccgccg ccgcctcgac ccggccgctg ctcgcggtgg gcggcatcac
     6121 gcccgagcgg gtgggtgagg tgcgcgcggc cggggccgcg ggcgtcgcgg tgatcggcgc
     6181 catctacgga gcggcgcgcc cggccgacgc caccaaggcc ttcctcgacg tgctgggcag
     6241 cgcatgatga cgtcgggccc ctcggtcctc gtgatcggcg gcggcatcat cggctgctcg
     6301 gccgcgtacg agctggcgaa gcggggctgc cgcgtcaccg tgctcgagcg cggcacaccg
     6361 ggaggcgagg cctcgggcgc ggcggccggg ctcctgtctc ccctcggcga ctcgccggat
     6421 ccgagcccgt tcaaccggct cgccatcgag agctggcgac tctaccctcg ggtcgtcgcc
     6481 gagctacggg aagtgaccgg ggtcgacgtg gagcacatga ccgcgggcac gctctatccg
     6541 ctcgcgagcg cggccggcgt cgaggacgcc cgcgctcgat gcggctggcc gctcgccgcc
     6601 gagttcggag tcgagatggt cgagggcacc gccctcgccg cgctcgagcc cgcgctcgcc
     6661 aaggacgtga ccgcggcgct cctggtgcgc ggcgaccact gggtgaacaa ccagcgcctg
     6721 gtcaccgcct acgcgctcgc cgcggcggcg cgcggggtga ccgtgcggac cggggtcgag
     6781 gtcggccgga tcctggtcga gcgcggccgc gcggccgggg tgctcgccga cggcgagcgg
     6841 ctcaccgccg acgcggtgct cctcgcggcg ggcgcctggt cgggcgggct cgcggccgac
     6901 ctgaggggtc ggctcccggt cggtccggtg cgcggacaga tgctcgcggt gagcaacgtg
     6961 ccggcgctga tctcccacgc gatgcacggc gacgacgtct acctggtccc gcgcccctcc
     7021 ggcgagctgc tgatcggcgc caccatcgag cgcgtgggat tcgagcgcgc ggtgacgccc
     7081 gaggggctgg gcgcgctcat cgcgcaggcg gtggcgctcg tgcccgagat cggccgccgc
     7141 gcgatcaccc gctcgtggta cggcttccgc cccgctgcgc cggacggcct gccggtgctc
     7201 gggccgtggc ccgacgtggc cgatctcttc gtggccaccg ggcactaccg caacggcatc
     7261 ctcctggccc cggtcaccgc ggcggtgatg gccgcctgca tcgtcgacga caagactccc
     7321 gactcgatca ccccgttcct tccccagcgc ttcgtgtcat aggagattcc cgtgcaaaca
     7381 aagaccggcc tcaagcagtc catctgcgaa gccatcgacc gcaacgcgga gcgcatcatc
     7441 gctctcggcg agcgcatccg gcgccagccc gagctcggct tcaaggagtt caagaccgcg
     7501 cgactggccg aggagacctt cagcgagctg ggcctgaagc cgcgaggcgg gctcgccctg
     7561 accggggtgc gcgcggaggc ggcgggggcc aaggacggcc cgaccttcgc gctgctcggc
     7621 gagctggacg gcctggtggt ggccggccac ccgatcgccg atccagagac gggggccgcg
     7681 cacgcgtgcg gccacaacgc gcagatggcg gggctgctcg gcgccgcaat gggcctgctc
     7741 gaggcgcgcg ccttcgagca cctggccggc cgggtggtct tctt
//
LOCUS       DATMCV010000957         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_318194, whole genome shotgun sequence.
ACCESSION   DATMCV010000957 DATMCV010000000
VERSION     DATMCV010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318194"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1362
                     /locus_tag="VNU03_21915"
     CDS             49..1362
                     /locus_tag="VNU03_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP77195.1"
                     /translation="MKRRTFLKIGSTALTASGLDGILAARRAPAFGQTTRIHLLHWPD
                     FIPEGDVELRRQIAEYNKQMKTDVFMENINGNDLQPRITAAIQTGAGPDIVFMLHNWP
                     HLYASSLADVSDLCEWKAKDQGGYYDQSEAAARDGSRWLALPYGIVGNQMAYRKSWFA
                     SVGATQPPKTLDEWRKIGAALKKKGKPIGQTLGHTYGDAPTFSYPLLWTFGGAETDKS
                     GKKVVLNSPGTVEAVKWMTAFWKDACDEGGLGWDDTSNNRAFHSGELCSTLNGASIYI
                     FAKRNPDKIKDEKGEPMYRDVGHFVYPDGPSGKTPTYHLVSSHAVMKYSKNVQAAKEF
                     LKHIHSKEQFTKWFEIDYGFNVGSTTYWENHPLWNTIDEAMKPYRYTGRGTRMIGHAG
                     PANAKASEVYSKYVITDMFAKAVQGMKAEDAVKWAEGELKRVYEA"
     gene            complement(1384..1638)
                     /locus_tag="VNU03_21920"
     CDS             complement(1384..1638)
                     /locus_tag="VNU03_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77196.1"
                     /translation="MRRMLGFPLAVGLLAGCATAAPRPAPPAGGLVPTACERSALMTG
                     QAPDVTSPEAAAENLRWGALAPESCPEPGRLDASPPGTRR"
     gene            complement(1672..>2172)
                     /locus_tag="VNU03_21925"
     CDS             complement(1672..>2172)
                     /locus_tag="VNU03_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77197.1"
                     /translation="GVRTGMETLVERGHLWLALVFVVVAAGVWATLFGLVRKRHLRNP
                     EGKILPLSAAGFLLGAAAVWVYVFAGLSYALMRLGFVEYALPPRPDDLLAHLTDAYSW
                     HFLDLLPGVGVNGALGWQCPVDLEGGRRGALLVLFRVAVLYQIFAKGRELLKRDESSG
                     APEANS"
BASE COUNT          396 a          777 c          727 g          272 t
ORIGIN      
        1 cgcgcgatcc gggcgcggct tcgaacccgc tgactacagg aggccccgat gaagcggcgc
       61 accttcctca agatcggcag caccgcgctg accgcgagcg ggctcgacgg catcctcgcc
      121 gcccggcgcg ctcccgcctt cggccagacc acgcggatcc atctgctcca ctggccggac
      181 ttcatccccg agggcgacgt ggagctgcgg cgacagatcg cggagtacaa caagcagatg
      241 aagacggacg tcttcatgga gaacatcaac ggcaacgacc tgcagccgcg catcaccgcg
      301 gcgatccaga ccggggcggg ccccgacatc gtcttcatgc tgcacaactg gccgcacctg
      361 tacgcgtcgt cgctcgccga cgtgagcgat ctctgcgaat ggaaggccaa ggaccagggc
      421 ggctactacg atcaatcgga ggccgccgcg cgcgacggca gccgctggct cgccctgccc
      481 tacggcatcg tgggcaacca gatggcctat cgcaagtcgt ggttcgcctc ggtcggggcg
      541 acccagccgc ccaagacgct ggacgagtgg cgcaagatcg gggccgcgct caagaaaaag
      601 ggcaagccca tcggccagac cctcggccac acctacggtg acgcgccgac cttctcctat
      661 cccctgctat ggaccttcgg gggcgccgag accgacaaga gtggcaagaa ggtggtgctc
      721 aactcgccgg gcaccgtcga ggcggtcaag tggatgaccg cgttctggaa ggacgcctgc
      781 gacgagggcg gcctcggctg ggacgacacc tcgaacaacc gcgccttcca ctcgggcgag
      841 ctctgctcga cgttgaacgg cgcctccatc tacatcttcg ccaagcgcaa cccggacaag
      901 atcaaggacg agaagggcga gccgatgtat cgggacgtgg gccacttcgt ctaccccgac
      961 ggccccagcg gcaagacccc gacctatcac ctggtctcct ctcacgcggt catgaagtac
     1021 tcgaagaacg tgcaggccgc gaaggagttc ctgaagcaca tccactcgaa ggagcagttc
     1081 accaagtggt tcgagatcga ctacggcttc aacgtggggt cgacgaccta ctgggagaac
     1141 catcccctgt ggaacacgat cgacgaggcg atgaagccct accgctacac cgggcgcggc
     1201 acccggatga tcggccacgc ggggcccgcc aacgcgaagg ccagcgaggt ctactccaag
     1261 tacgtgatca ccgacatgtt cgccaaggcc gtccagggca tgaaggcgga ggacgcggtg
     1321 aagtgggcgg agggcgagct caagcgggtc tacgaagcgt aggcggcggc cgggaggccg
     1381 ggctcagcgc cgggtcccgg gcgggctcgc gtccagtcgc cccggctccg gacaggactc
     1441 gggcgcgagg gcgccccagc gcaggttctc ggccgcggcc tccgggctgg tgacgtcagg
     1501 cgcctggccg gtcatgaggg cgctccgctc gcacgccgtc ggcacgaggc cgccggccgg
     1561 cggcgccggc cgcggcgcgg cggtcgcgca gcccgccagc agacccaccg ccagcgggaa
     1621 cccgagcatc cgcctcatgt cgtcctccga tcgcccggtg gtgcattccc gtcacgagtt
     1681 ggcttccggc gccccgctgc tctcgtcccg cttcaggagc tcccgcccct tggcgaagat
     1741 ctggtagagc accgcgaccc ggaacagcac gagcagcgcg ccgcggcggc ctccctcgag
     1801 gtcgaccggg cactgccatc cgagcgcgcc gttgacgccg acgcccggca gcaagtcgag
     1861 gaagtgccag gagtacgcgt cggtgagatg ggcgagcaga tcgtccggcc gcgggggcag
     1921 cgcgtactcc acgaagccca gccgcatcag cgcgtaggag agcccggcga agacgtacac
     1981 ccacaccgcg gcggcgccga gcaggaaccc ggccgccgac agcggcagga tcttgccctc
     2041 ggggttccgg aggtgacgct tgcggaccag gccgaacagc gtcgcccaca cccccgccgc
     2101 caccaccacg aagacgagcg cgagccagag gtggccccgc tccacgagcg tctccatgcc
     2161 ggtccgcacg cc
//
LOCUS       DATMCV010000958         4693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_119106, whole genome shotgun sequence.
ACCESSION   DATMCV010000958 DATMCV010000000
VERSION     DATMCV010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119106"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VNU03_21930"
     CDS             <1..555
                     /locus_tag="VNU03_21930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77198.1"
                     /translation="KEVGKGTGLGLATVYGIVKQSGGLIEVESEPGHGATFKVYLPAV
                     AAPVEAAEPAKAPGTRMRGSETVLLVEDDPNLRTLAREILTVQGYTVIEAASPLEAIR
                     IHQSHADAIHLLLTDVVTPEMNGPQLAAQLQAGRPGMEVLFMSGYTGSALALEGGGEF
                     GGAFLAKPFTPDGLSRRVREVLDA"
     gene            complement(552..1409)
                     /locus_tag="VNU03_21935"
     CDS             complement(552..1409)
                     /locus_tag="VNU03_21935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWP77199.1"
                     /translation="MARAGRPLDRQRWALTAALLALAVLWLSPIAWVVVTSLKPSENI
                     IRLPPEWIPWPATGAHYAEVLWGSSRTARIGRAFANSLVVATGSVLVVLATSAMAAYP
                     LARMRFPGRDLVFVVLVGSLMIPNAVLLVPQYVLTQRLGWLSTYQGLIVPEAAMIFAF
                     GVFLLRQFFLTMPRELEDAALIDGAGPWQVFSRIILPLSQPVLAALAIFAFRSAWNDF
                     LWPLIAVNKPEMFPLPVALALLRSAYSSESYGPIMAGAALSALPLLVVFVVANRRIVE
                     GVRLSGLKG"
     gene            complement(1413..2279)
                     /locus_tag="VNU03_21940"
     CDS             complement(1413..2279)
                     /locus_tag="VNU03_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWP77200.1"
                     /translation="MSRQTRVAYAFLAPGLLLFAAYRLYPLLEGLRLSFTNARLGRPT
                     QQWVGLANYARLLEDTRFQVSLWNTAFYTVASTLPILAVPLVLALALNRGRLKNLLRS
                     VFFFPFTLSVVTVGLAWLWLLDPVVGPFNYYLRALGAPARSWLADPHTAMWAIILTTV
                     WWVTGYYLVVYLAGLQDIPRELYEAAALDGASGVRAFWAITLPLLRPVLLFVVVTHVI
                     GSFQLFGQVFVLTGGGPGDATRTVVQHLYETAFQNFFQFGSASAMAWVLFAVILAFSL
                     LQFRLLRGHTEY"
     gene            complement(2281..3609)
                     /locus_tag="VNU03_21945"
     CDS             complement(2281..3609)
                     /locus_tag="VNU03_21945"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77201.1"
                     /translation="MSGHDNGVSDANGSNGAATRGTARRARVSRRAFVAGAGAASVAA
                     WARRAGAQPKATITYWNGLTGADGKVMDELIDQFTRDSGIRIEQQRLPWADLYAKLQV
                     AVPAGEGPDLALIHTVEVPHFASDGVLEVIDDATATGKGFRGEDYLPAMWQGGTFQGK
                     RYSLPLDVPQHILYMNVKVMKDAGLVGSDGRPRVPASRDELVTMAKRIAAKADTFGFA
                     IGTVTPGRYTWGFHNLLWQNGANVYNVDLKRAGVSDPAALEVAEFWAALEGQHKIAPP
                     ANANCRDAFIAGKLGMWIAGSWNFTGLREAKVEFVAVPVPRLFKQAVVWAMPHQYTFP
                     KPKAADRGKRDAAWAHVRWMTDHVAEWTLKAGQVSASRKAHSDPRVTADPVLRALLSQ
                     APSWQVGQPTPKWVAAENLTRPVIEAVYTGQKPPKAAMEDLARQINALPD"
     gene            3698..>4693
                     /locus_tag="VNU03_21950"
     CDS             3698..>4693
                     /locus_tag="VNU03_21950"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HWP77202.1"
                     /translation="MTTRIGIDLRRRIGTVDPRIFGQFIEHLGRCIYGGVYDEGSPRS
                     DSRGFRLDVLEAARPLRFPVLRWPGGNFVSGYHWLDGVGPKESRPRRSELAWYAEESN
                     RFGTNEFIEYCRELGTEPIICVNMGSGTMDEAQAWVEYCNGVGDTSWANLRRSHGYPE
                     PHRVRYWGLGNEMYGGWQIGSLEAHDYVKKARAFGMVMKRTDPSIQLVGCGLNGWSQW
                     DEVVLDGLAEMVDFHSIHLYTGGVDHYTTVMQSHQAERAVRICAALIERVRHMRQIAH
                     PIHIAFDEWNVWWRTRSHADRVGGVEERYTLTDALAVATYLHGFIRQCRVVRMANF"
BASE COUNT          741 a         1557 c         1759 g          636 t
ORIGIN      
        1 aaggaggtgg ggaaggggac ggggctgggg ctcgccaccg tgtacggcat cgtgaagcag
       61 agcggcgggc tgatcgaggt cgagagcgag ccgggccacg gcgcgacctt caaggtgtac
      121 ctgcccgcgg tggcggcgcc ggtcgaggcc gcggagccgg ccaaggcgcc gggcacgcgg
      181 atgcgcggct cggagaccgt gctgctggtc gaggacgatc cgaatctgcg cacgctggcc
      241 cgcgagatcc tgaccgtgca gggctacacc gtgatcgagg ccgcgtcgcc cctggaggcg
      301 atccgcatcc accagagcca cgcggacgcg atccacctcc tgctcaccga cgtggtcacg
      361 ccggagatga acgggcccca gctcgcggcg cagctgcagg cgggccggcc cggcatggag
      421 gtgctgttca tgtcgggcta caccgggtcc gccctggccc tggagggcgg gggcgagttc
      481 ggcggcgcgt tcctggcgaa gcccttcacg ccggacggtt tgagccgccg cgtgcgcgag
      541 gtcctcgacg cctagccctt gaggccgctc aggcgcacgc cttccacgat gcgccggttg
      601 gccaccacga agaccaccag cagcggcagc gcgctcagcg ccgcgcccgc catgatcggc
      661 ccgtaggact cggagctgta ggcgctccga agcagggcga gcgccaccgg cagcgggaac
      721 atctcgggct tgttcaccgc gatgagcggc cacaggaaat cgttccacgc cgagcggaac
      781 gcgaagatcg cgagcgcggc cagcaccggc tgcgagagcg gcaggattat ccgggagaag
      841 acctgccagg ggcccgcgcc gtcgatgagg gctgcgtcct ccagctcacg cggcatcgtg
      901 aggaagaact gccgcagcag gaacaccccg aacgcgaaga tcatcgccgc ctccggcacg
      961 atcaggccct ggtaggtgct gagccagccg agccgctggg tgagcacgta ctggggaacc
     1021 agcagcaccg cgttcgggat catcaggctg cccaccagca ccacgaagac caggtcacgc
     1081 ccggggaagc gcatgcgggc gagcgggtag gcggccatcg cgctggtggc gaggacgacc
     1141 agcacgctgc cggtggccac caccaggctg ttggcgaacg cgcgcccgat gcgggcggtg
     1201 cgggaggagc cccacagcac ctcggcgtag tgggcgccgg tcgcgggcca tgggatccac
     1261 tcgggcggga gccggatgat gttctcgctc ggcttgagcg aggtcacgac cacccacgcg
     1321 atcggggaga gccagaggac ggcgagcgca agcagcgccg cggtgagggc ccagcgctga
     1381 cggtcgagcg ggcggcccgc gcgcgccatc gctcagtact cggtgtggcc gcgcagcagg
     1441 cggaactgga gcagcgagaa ggcgaggatg accgcgaaga gcacccacgc catcgcggag
     1501 gcggagccga actggaagaa gttctggaac gcggtctcgt agagatgctg gacgacggtg
     1561 cgggtggcgt cgccggggcc gcccccggtg aggacgaaca cctgaccgaa gagctggaac
     1621 gatccgatca cgtgcgtgac caccacgaag agcagcaccg ggcggagcag cggcagggtg
     1681 atcgcccaga acgcgcggac gccggaggcc ccgtcgagcg cggccgcctc gtacagctcg
     1741 cgcgggatgt cctggagccc ggccaggtag accaccaggt agtagccggt cacccaccac
     1801 acggtggtca ggatgatcgc ccacatcgcg gtgtgcggat cggcgagcca ggagcgggcg
     1861 ggcgccccga gggcgcgcag gtagtagttg aaggggccca ccaccggatc gagcagccag
     1921 agccaggcga ggccgacggt gaccaccgag agcgtgaagg ggaagaagaa gacgctgcgc
     1981 aggaggttct tcagccggcc gcggttcagc gcgagggcca gcacgagcgg caccgcgagg
     2041 atcggcagcg tgctggccac cgtgtagaac gcggtgttcc agaggctcac ctggaagcgg
     2101 gtgtcctcga gcaggcgcgc gtagttggcg agcccgaccc actgctgggt cgggcggccg
     2161 agccgcgcgt tggtgaagga gagccgcagg ccctcgagga gcgggtagag gcgataggcc
     2221 gcgaagagga ggaggcccgg cgccaggaag gcgtaggcga cgcgggtctg gcgcgacacg
     2281 ctagtcgggc agcgcgttga tctggcgggc cagatcctcc atcgcggcct tgggcggctt
     2341 ctgcccggtg tagaccgcct cgatcaccgg ccgggtcagg ttctcggccg ccacccactt
     2401 gggagtgggc tggccgacct gccagctcgg agcctgggag agcagcgcgc gcagcaccgg
     2461 gtcggcggtg acgcgcgggt ccgagtgcgc cttgcggctc gccgagacct ggccggcctt
     2521 gagcgtccac tccgccacgt ggtcggtcat ccagcgcacg tgggcccacg ccgcgtcgcg
     2581 cttgccgcgg tcggccgcct tcggcttggg gaaggtgtac tggtggggca tcgcccacac
     2641 caccgcctgc ttgaacaggc gcggcaccgg caccgccacg aactccacct tggcctcgcg
     2701 caggccggtg aagttccagg agcccgcgat ccacatgccg agcttgcccg cgatgaaggc
     2761 gtcgcggcag ttggcattgg cgggcggggc gatcttgtgc tggccttcga gcgcggccca
     2821 gaactcggcc acctcgagcg cggccggatc gctgacacct gcgcgcttga gatccacgtt
     2881 ataaacgttc gccccgttct gccagagcag gttgtggaag ccccaggtgt agcggcccgg
     2941 ggtcacggtg ccgatcgcga agccgaaggt gtcggccttg gccgcgatgc gcttggccat
     3001 ggtcaccagc tcgtcgcggc tggccggcac gcgtggccgc ccgtccgagc cgacgaggcc
     3061 cgcgtccttc atcaccttca cgttcatgta gaggatgtgc tggggcacgt cgagcggcag
     3121 cgagtagcgc ttgccctgga aggtgccgcc ctgccacatc gcgggcaggt agtcctcgcc
     3181 gcggaagccc ttgccggtgg cggtggcgtc gtcgatgacc tcgagcacgc cgtcgctggc
     3241 gaagtggggg acctcgacgg tgtggatgag ggcgagatcg gggccctcgc cggccgggac
     3301 cgcgacctgc agcttggcgt agaggtcggc ccagggcagg cgctgctgct cgatgcggat
     3361 gccgctgtcg cgggtgaact ggtcgatcag ctcgtccatc accttgccgt cggccccggt
     3421 gaggccgttc cagtaggtga tggtggcctt cggctgcgcg cccgcccggc gagcccacgc
     3481 ggccacggag gccgcgcccg ctccggcgac gaaggctcga cggctcacgc gcgcgcgtcg
     3541 cgcggtgccg cgcgtggcgg ccccgttgct gccgttggcg tcgctgactc cgttgtcgtg
     3601 cccgctcatt ggcgtatcct ccgggcgaga ctgtacggcc ccgcgcgcca cactgtcaaa
     3661 ttgacaggcc cggagcccgc tcctagactc tggcgccatg accacgcgca tcggcatcga
     3721 cctccggcgg cgcatcggca cggtggaccc gcggatcttc ggccagttca tcgagcatct
     3781 cggccgctgc atctacggcg gcgtgtacga cgaaggctcc ccgcgcagtg actcgcgcgg
     3841 ttttcgtctc gatgttctgg aggcggcgcg cccgctccgc ttcccggtgc tccgctggcc
     3901 gggcggtaac ttcgtgagcg gctaccactg gctcgacggg gtggggccga aggagtcgcg
     3961 gccccggcgc agcgagctcg cgtggtacgc ggaggagtcg aaccgcttcg gcaccaacga
     4021 gttcatcgag tactgccgcg agctggggac cgagccgatc atctgcgtga acatgggctc
     4081 cggcaccatg gacgaggcgc aggcgtgggt cgagtactgc aacggcgtag gcgacacctc
     4141 gtgggccaac ctgcgccgga gccacggcta cccggagccg caccgcgtcc gctactgggg
     4201 gctcggcaac gagatgtacg ggggctggca gatcggcagc ctcgaggcgc acgactacgt
     4261 gaagaaggcg cgcgcgttcg gcatggtgat gaagcgcacc gacccgtcca tccagctggt
     4321 gggctgcggc ctgaacggct ggagccagtg ggacgaggtg gtgctggacg gcctcgccga
     4381 gatggtggac ttccactcca tccacctcta caccggcggc gtcgaccact acaccacggt
     4441 gatgcagtcg caccaggcgg agcgggcggt gcgcatctgc gccgcgctga tcgagcgggt
     4501 gcgccacatg cggcagatcg cgcacccgat ccacatcgct ttcgacgagt ggaacgtgtg
     4561 gtggcgcacg cggagtcacg cggaccgggt gggcggggtc gaggagcgct acacgctgac
     4621 cgacgcgctc gcggtggcga cctatctgca cggcttcatc cggcagtgcc gggtggtgcg
     4681 catggccaac ttc
//
LOCUS       DATMCV010000959         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_50508, whole genome shotgun sequence.
ACCESSION   DATMCV010000959 DATMCV010000000
VERSION     DATMCV010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50508"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VNU03_21955"
     CDS             complement(<1..699)
                     /locus_tag="VNU03_21955"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77203.1"
                     /translation="MRLAQKIAIVTGTSPNIGGGIAEGLAEEGATVVCVDVAADNAGQ
                     CAEAIRRAGGQALGIPCDVTDEGQVEAMVARVRQTYGGVDILVNNAGILGGLGVLEMP
                     VERWSRQIAVNLTGTFLCTKHVARLMVAEGRRGSIVVVVSTAGHQGQAGNIAYSTSKS
                     GLLNFTRAAAMDLARHGIRVNSLTPTATDTEEAAERAVAWGRPRPEPRPRGLDFPKMV
                     PMGKLPSPRHYARAV"
     gene            complement(710..>2104)
                     /locus_tag="VNU03_21960"
     CDS             complement(710..>2104)
                     /locus_tag="VNU03_21960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWP77204.1"
                     /translation="WVEFAFWAAFFLPPFTVTLSWILLLDPEYGLVNTALARLPLVTS
                     GPFNIYSFWGIVWVHVLTGSLTVKVILLTPAFRNMNAAFEEASRVAGAGTLRTALRIT
                     VPVMAPAILSVLLLGTMVSLQTFEVEQVLGLPFRFFVFSTTIYDLLVTRVPRYDAATA
                     LAVLILAAMLPLVFAQQWLTRGRGYTTVTGQFQRRVHALGRWRAPATVFVMALVVVVL
                     GAPVLFALLGTFMKLFGFFHVPDPWTLANWQTVLGDDVFLRSLHNTLVLSIGTAVAAI
                     VVHSLIAYIIVRTSYAGRRLLDLVSWLPFTVPGIILGLALLWLFLGVPFLRPLYGTTA
                     LLIVAGVIAGMPLGVQIIRSGLLQLGGELEEASRIAGASWWTTYRRIILRLMAPTLIA
                     VGMITFVGAARNIGNFALLTTSANRPLSILQLDYVAQRKFEEAVVVACIIMLVSLAGA
                     LLARLIGLRSGGLG"
BASE COUNT          355 a          700 c          791 g          258 t
ORIGIN      
        1 gaccgcgcgg gcgtagtggc gggggctcgg cagcttgccc atcgggacca tcttcgggaa
       61 gtcgaggcca cgcgggcgcg gctcggggcg ggggcggccc caggcgaccg cgcgctcggc
      121 ggcttcctcg gtgtcggtgg cggtgggggt caggctgttc acccggatgc cgtggcgggc
      181 caggtccatg gcggcggccc gcgtgaagtt gagcagcccg ctcttgctcg tcgagtaggc
      241 gatgttgccg gcctgcccct gatggccggc cgtcgagacc accaccacga tgctgccgcg
      301 ccgcccctcg gccaccatca gccgcgccac gtgcttggtg cagaggaagg tcccggtcag
      361 gttgaccgcg atctggcggc tccagcgctc caccggcatc tcgagcacgc cgaggccccc
      421 gaggatgccc gcgttgttca cgaggatgtc gacgccgccg taggtctggc gcacgcgcgc
      481 gaccatcgcc tccacctggc cctcgtcggt cacgtcgcag ggaatgccga gggcctggcc
      541 cccggcgcgg cggatcgcct ccgcgcactg gcccgcgttg tccgccgcca cgtccacgca
      601 caccacggtg gcgccttcct cggcgaggcc ctcggcgatg ccgccgccga tgttggggct
      661 ggtcccggtc acgatcgcga tcttctgggc tagacgcacg ggcactccat cagccgagcc
      721 cgccgctgcg cagcccgatc agccgcgcga gcagagctcc ggccagcgag accagcatga
      781 tgatgcacgc cacgaccacc gcctcctcga acttgcgctg ggccacgtag tcgagctgga
      841 ggatcgacag cggccggttg gcgctggtgg tgagcagggc gaagttgccg atgttgcgcg
      901 cagcgcccac gaaggtgatc atgcccaccg cgatcagggt cggggccatc aggcgcagga
      961 tgatccggcg ataggtcgtc caccacgacg cgcccgcgat ccgggaggcc tcctccagct
     1021 cgccgcccag ctgcaggagc ccgctccgga tgatctgcac gccgagcggc atgcccgcga
     1081 tgacccccgc gacgatcagg agggcggtgg tgccgtacag cggccgcagg aaggggaccc
     1141 cgaggaagag ccagagcagg gccaggccca ggatgatgcc gggcaccgtg aacggcagcc
     1201 acgagacgag atcgagcagc cgccggcccg cgtaggaggt gcgcacgatg atgtaggcga
     1261 tgagcgagtg caccacgatc gcggccaccg cggtgccgat cgagagcacc agcgtgttgt
     1321 gcagcgagcg caggaacacg tcgtcgccga ggacggtctg ccagttggcg agcgtccacg
     1381 ggtccggcac gtggaagaag ccgaagagct tcatgaaggt gccgaggagc gcgaacagca
     1441 ccggcgcgcc caggacgacg accaccaggg ccatcacgaa gacggtggcg ggggcgcgcc
     1501 accggccgag ggcgtggacc cggcgctgga actggccggt cacggtggtg tagccccggc
     1561 cgcgggtcag ccactgctgg gcgaagacga gcgggagcat ggcggccagg atcaggaccg
     1621 cgagcgcggt ggccgcgtcg tagcgcggga cccgggtgac gaggaggtcg tagatcgtgg
     1681 tgctgaacac gaagaagcgg aagggcaggc ccagcacctg ctccacctcg aaggtctgca
     1741 gcgacaccat ggtgccgagc agcagcaccg acaggatcgc gggagccatc accgggaccg
     1801 tgatccggag cgcggtccgg agggtgcccg ccccggccac ccgggaggcc tcctcgaagg
     1861 ccgcgttcat gttgcggaac gcgggggtga ggaggatgac cttgacggta agggatccgg
     1921 tgaggacgtg gacccagacg atgccccaga acgagtagat gttgaagggg cccgacgtca
     1981 cgagcggcag ccgggcgagc gcggtgttga cgaggccgta ctcggggtcg agcagcagga
     2041 tccagctgag agtcaccgtg aacgggggca ggaagaaggc ggcccagaac gcgaactcga
     2101 ccca
//
LOCUS       DATMCV010000960         2449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_391752, whole genome shotgun sequence.
ACCESSION   DATMCV010000960 DATMCV010000000
VERSION     DATMCV010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391752"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..941)
                     /locus_tag="VNU03_21965"
     CDS             complement(27..941)
                     /locus_tag="VNU03_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWP77205.1"
                     /translation="MSVRSRREILRLGAAALGGLVVSQPARGQGTLERAVPRTKERLP
                     AVGLGTWQVFDVAGDARELGQARETLRVFTDGGGRVIDSSPMYGSSEAVTGELAVELG
                     VQAKLFVATKVWTSGRRAGIQQMEDSMRKLRVERLDLMQVHNLVDVQTHLGTLAEWKA
                     AGRVRYVGITHYHAGGHAELERLLRRGDVDFVQVNYSLAEPQADARLLGAAADARVAV
                     IVNRPFAEGAMFGRVKGKAVPEWAQAVGCASWAQYFLKWILAHPAVTCVIPGTRNPRH
                     AADNLGAMAGPLPDAATRRRMREHFDSL"
     gene            complement(938..1750)
                     /locus_tag="VNU03_21970"
     CDS             complement(938..1750)
                     /locus_tag="VNU03_21970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP77206.1"
                     /translation="MPALSVGERDSVAYEDTGRGPLVVLIHGSPGSARVWQPVTEQLA
                     SRFRVIAPNLPGYGATTRPPAGLPSDSGHAARLIEALLDGMKPPAVVAGHSYGGVVAL
                     LLALRGTVKPGALALFEPVAVPLLAAVGETAAFASARALFDDYRAAFEAGDRLAVRKM
                     IDYWFGPGSYDQLPTPAREYLVNYTSNNIDDVTATFRDTYSVDALRRLTVPTLVAYGA
                     RSPETMVKIVQAIAALVPGARLERIEDANHALTTTHADLVAAMIATLADRSA"
     gene            complement(1760..>2449)
                     /locus_tag="VNU03_21975"
     CDS             complement(1760..>2449)
                     /locus_tag="VNU03_21975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP77207.1"
                     /translation="AGLARRGVRPGAAVLDAGCGTGRYAVELARRGFAVTGLDRASEL
                     LDEARSRARAAGLPITLLEADLLDLPATPGGRLHDAIVCRGVLNDVVGPAARATVLGG
                     FARMLRAGGVVWLDVRDWSRTAVRKTAEPVTERHVATPRGPLAFRSVTRLDPATRRLL
                     VSERHSLTTPAGERVATHEFVMQCWTRDELNGALGAAGFEAIEHAPGETGEPGAGFED
                     RLVVTASRRAR"
BASE COUNT          308 a         1002 c          831 g          308 t
ORIGIN      
        1 gcatggctcc tcctgactgc tacggattat agcgaatcga agtgctcgcg catgcgccgc
       61 cgcgtcgccg cgtccggcag cgggcccgcc atcgcgccca ggttgtccgc cgcgtgccgc
      121 ggattgcgcg tgcccgggat cacgcaggtg accgccgggt gcgccaggat ccacttgagg
      181 aagtactgcg cccagctcgc gcagccgacc gcctgcgccc actccgggac cgccttcccc
      241 ttcacccgcc cgaacatcgc cccctccgcg aacggccggt tcacgatcac cgccacgcgc
      301 gcgtcggcgg cggcccccag caggcgcgcg tccgcctgcg gctcggccag cgagtagttc
      361 acctgcacga agtcgacgtc gccccggcgc aggaggcgct ccagctcggc gtgcccgccc
      421 gcgtggtagt gggtgatgcc gacgtagcgc acgcgcccgg ccgccttcca ctccgcgagc
      481 gtccccaggt gcgtctgcac gtccaccagg ttgtgcacct gcatcaggtc gagccgctcg
      541 acgcgcagct tccgcatcga gtcctccatc tgctggatgc cggcccgccg gccactcgtc
      601 cacaccttgg tggcgacgaa gagcttcgcc tgcacgccca gctcgacggc gagctctccg
      661 gtcaccgcct cggagctccc gtacatcggc gagctgtcga tcacccgtcc gcccccgtcc
      721 gtgaagaccc gcagcgtctc gcgggcctgc cccagctccc gcgcgtcgcc cgccacgtcg
      781 aacacctgcc aggtcccgag gccgaccgcg ggcagccgct ctttcgttcg cggcacggcc
      841 cgctcgagcg tcccctgccc gcgcgccggc tggctcacca ccagccctcc cagggccgcc
      901 gccccgagcc ggaggatctc ccgccgcgac cgcacactca cgcgctccgg tcggcgagag
      961 tcgcgatcat cgccgccacc aggtccgcgt gggtcgtggt cagcgcgtgg ttggcgtcct
     1021 cgatccgctc gagccgcgcg ccgggcacga gcgccgcgat ggcctgcacg atcttcacca
     1081 tggtctccgg gctccgggcg ccgtaggcca ccagcgtcgg caccgtgagg cgccggagcg
     1141 cgtcgaccga gtaggtatcc cggaaggtgg cggtcacgtc gtcgatgttg ttgctcgtgt
     1201 agttcaccag gtactcgcgc gccggcgtcg gcagctgatc gtaggatccc ggcccgaacc
     1261 agtagtcgat catcttgcgt accgccaggc gatcgcccgc ctcgaacgcg gcgcggtagt
     1321 cgtcgaagag cgcccgggcg gaggcgaagg ccgccgtctc gccgaccgcg gcgagcagcg
     1381 ggaccgccac cggctcgaac agggcgagcg cgcccggctt caccgtgccc cgcagcgcga
     1441 gcagtagcgc caccaccccg ccgtaggagt ggccggccac caccgcgggc ggcttcatgc
     1501 cgtcgagcag cgcttcgatc agccgcgccg cgtgcccgct gtcgctcggc aggcccgcgg
     1561 gcggccgggt ggtcgccccg tagccgggca ggttcggcgc gataacccgg aagcgcgacg
     1621 cgagctgctc ggtcaccggc tgccagaccc gcgcgctccc cggcgagccg tggatcagca
     1681 cgacgagcgg gccgcggccg gtgtcctcgt aggccaccga gtcgcgctcg cccacgctca
     1741 gcgccggcat cgccgccccc taccgcgcgc ggcgcgaggc ggtgaccacg aggcgatcct
     1801 cgaagcccgc gcccggctcg cccgtctcac cgggcgcgtg ctcgatcgcc tcgaagccgg
     1861 ccgcgcccag cgcgccgttc agctcgtccc gggtccagca ctgcatcacg aactcgtgcg
     1921 tggcgacccg ctcccccgcc ggcgtggtca ggctgtggcg ctccgagacc agcagccgcc
     1981 gcgtcgccgg atcgaggcgc gtgacgctcc ggaacgcgag cggcccccgc ggggtggcga
     2041 cgtgcctctc ggtcacgggc tcggccgtct tgcggaccgc ggtgcggctc cagtcgcgca
     2101 cgtcgagcca caccacccca cccgcacgca gcatccgggc gaacccgccg agcactgtcg
     2161 cgcgggcggc cgggccgacc acatcgttga ggacgccgcg gcacacgatc gcgtcgtgga
     2221 gccgtccgcc cggcgtggcc ggcaggtcca gcaggtccgc ctcgagcagc gtgatcggca
     2281 gacccgcggc ccgcgcacgg ctccgcgcct cgtccagcag ctcggacgcg cgatcgagcc
     2341 cggtcaccgc gaagccgcgc cgcgccagct cgaccgcgta gcggccggtc ccgcacccgg
     2401 cgtcgagcac cgcggccccc ggccggacgc cgcgccgcgc cagcccggc
//
LOCUS       DATMCV010000961         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_518737, whole genome shotgun sequence.
ACCESSION   DATMCV010000961 DATMCV010000000
VERSION     DATMCV010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518737"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1085
                     /locus_tag="VNU03_21980"
     CDS             135..1085
                     /locus_tag="VNU03_21980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77208.1"
                     /translation="MTTERAWTLGILLALGLLAFLPLLAPRDDVLNFVFLVLLSITLA
                     QSWNIIAGYAGQVNLGHAAFFGLGALTARTLWIAGTPVVAAMAAGAAVATTFALLIGA
                     AAFRLRGAYFAIGTLALGEILRTTVGNALPEISTLPAASIGAYRLAPRYYLALALAAL
                     SVLAVAGLARSRLGLGMVAVREDEDAAEATGVSALALKLRALALSTALAGAAGGVFAY
                     YHISYYPSHPFGPGWTFDALLMTFIGGVGTLHGPVLGAVLYVFLKEYLTVRWVDFHLL
                     IFGVLFIAIVLIFPGGLVQALIQAGNRARGRFARRPGATT"
     gene            1082..1471
                     /locus_tag="VNU03_21985"
     CDS             1082..1471
                     /locus_tag="VNU03_21985"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP77209.1"
                     /translation="MTTRIPLLELGSLQSRLPELIAKKGEPPWSDALVLTDDIQAFLI
                     CHPPGQPNDTHYHHHDEWWVVLQGEIDWYIEGQAGPIHARAGDFVFGPKHLWHHLEPV
                     GTAPSIRIAINARGEFHRYDRPGCKPL"
     gene            1530..>2365
                     /locus_tag="VNU03_21990"
     CDS             1530..>2365
                     /locus_tag="VNU03_21990"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77210.1"
                     /translation="MRRRDFIIFLAGAMAAWPLAARAQQKAMPVIGVLNTGSPSASSG
                     PTMAAFRQGLSEAGYVEGQNLAIEYRWAEGHYDRLPTLAADLVGRKVDLIMANSPPSA
                     LAAKSATSTIPIVFRSGADPVGDGLVASLARPGGNLTGVSFVADGLTAKRLELLSELV
                     PRARVIALLMNPSNATAERVIRDVQEAARTNGLQLHVLKARSESEIDTAFASLVQLHA
                     GALVVGADPFLSSQREQLVALASRRAVPSIYAWREFAASGGLISYGPSITSAFRLVGA
                     YAG"
BASE COUNT          324 a          847 c          787 g          407 t
ORIGIN      
        1 gccggcctct tcgtgggcgt ggccgaggcc ctcagcgcgg ccgccttcgg cgggccctac
       61 cgcgaggtga tcggtctggt catcttcctg gtagtcctgg tcctcaggcc ccaaggcctg
      121 ttcgggcgcg cccgatgacg acggagcgcg cctggactct gggcatcctc ctcgcgcttg
      181 gcctcctcgc cttcctgccg ctgctcgcgc cccgcgacga cgtgctcaac ttcgtgttcc
      241 tcgtcctcct ctcgatcaca ctggcccaga gctggaacat catcgcgggc tacgcggggc
      301 aggtgaacct gggccacgcc gccttcttcg gtctcggcgc gctcaccgcg cgcacgctgt
      361 ggatcgcggg cacgccggtg gtggccgcga tggccgctgg cgcggcggtc gccaccacgt
      421 tcgcgctgct gatcggcgcc gccgcgttcc ggctgcgcgg cgcctacttc gcgatcggca
      481 cgctggccct gggcgaaatt ttacggacca ccgtcggcaa cgcgctgccc gagatctcca
      541 ccctgcccgc ggccagcatc ggcgcctacc gcctcgctcc ccgctactac ctggcgctgg
      601 ccctcgccgc gctctcggtg ctcgcggtgg ccgggctcgc ccgctcgcgg ctcgggctcg
      661 gcatggtggc cgtccgcgag gacgaggacg cggcggaggc gaccggggtg tccgcgctcg
      721 cgctcaagct gcgggcgctc gcgctgtcga ccgcgctggc cggcgcggcg ggcggggtct
      781 tcgcgtacta tcacatcagc tactacccgt cgcacccgtt cggacccggc tggacgttcg
      841 acgcgctgct catgaccttc atcggcggcg tcggcacgct gcacgggccc gtgctgggcg
      901 cggtgctcta cgtgttcctg aaggagtacc tgaccgtgcg ctgggtggac ttccacctct
      961 tgatcttcgg cgtgctcttc atcgccatcg tgctcatctt ccccggcggg ctggtccagg
     1021 ccctgatcca agccgggaac agggcgcggg ggcggttcgc ccgccgaccc ggagcgacga
     1081 catgaccaca cgcatcccgc tgctcgagct ggggtcgctg caaagccgtt tgccggagct
     1141 gatcgccaag aagggcgagc cgccctggtc ggacgcgctc gtgctgaccg acgacatcca
     1201 ggcgttcctg atctgccacc cgcccggcca gcccaacgac acgcactacc accatcacga
     1261 cgagtggtgg gtggtgctgc agggcgagat cgactggtac atcgaggggc aggccgggcc
     1321 gatccacgcg cgcgcgggcg acttcgtctt cggccccaag cacctctggc accacctgga
     1381 gccggtcggc accgcgccca gcatccgcat cgccatcaac gcccgcggcg agttccaccg
     1441 ctacgaccgc ccaggctgca agcccctctg acggggtcag gtcttacatt atgccgtcta
     1501 cgcactacgc acagagcgag aggccgggca tgaggcgacg cgatttcata atttttctcg
     1561 ccggcgcgat ggcggcatgg ccgctcgctg cgcgcgcgca gcagaaggcg atgccggtga
     1621 tcggcgtcct caacaccggg tcgcccagcg cgtcttctgg accgactatg gccgcgttcc
     1681 gccagggact gagcgaagcc ggctacgtcg agggacagaa tttggcgatc gaataccgct
     1741 gggcggaggg ccactacgat cggctgccca cattggccgc cgatctcgtc ggccgcaagg
     1801 tcgatttgat catggcgaac agcccgcctt cggcactagc cgcgaaaagc gcgacctcga
     1861 cgatcccgat cgtcttcagg agtggcgccg acccggtcgg ggacgggctg gtcgccagcc
     1921 tcgcccggcc gggcggcaac ctcacggggg tcagcttcgt cgcggacggg ctgacggcta
     1981 agcggcttga gctgctgtct gagctggttc cgcgggctcg ggtgattgcc ctgctgatga
     2041 acccgagcaa tgcgactgcc gagcgcgtca ttcgagacgt gcaggaagca gcgcgcacga
     2101 atgggttgca gctccatgtc ctgaaagccc gcagcgaaag cgagatcgac accgccttcg
     2161 cctccctagt ccaactgcat gctggcgcgc tcgtcgtcgg cgcggatccg ttcttgagca
     2221 gccagcgcga gcagctcgtg gcgctggcat cgcgccgtgc cgttccgtcg atctatgcgt
     2281 ggcgtgaatt cgccgcttcc ggcggcctga tcagctatgg accaagcata acttctgcct
     2341 ttcgcctggt cggtgcttac gcggg
//
LOCUS       DATMCV010000962         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_785863, whole genome shotgun sequence.
ACCESSION   DATMCV010000962 DATMCV010000000
VERSION     DATMCV010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785863"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VNU03_21995"
     CDS             <1..436
                     /locus_tag="VNU03_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77211.1"
                     /translation="EYYRLLAPGGHIAILDTPNRLFPLETHSIGLPLVQWLPPRLAHA
                     YARVARPRKFARVSFETFVADGTGWRNATLSDCLPSRGPVGLEDVTEAAGYGWRFFRD
                     TARSRVRRALLPLFGAAAGALRAAGRPPSLCLPYLNLLLRKQ"
     gene            433..1317
                     /locus_tag="VNU03_22000"
     CDS             433..1317
                     /locus_tag="VNU03_22000"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP77212.1"
                     /translation="MIRIEIGRRHYLLYLLLPHPLKSRLLTAGSRAKLERINRSFNDT
                     IYSGTGASAYDEIHRYAEDEQHEYPARPLVDRVWPGAAGYGLALEVGAGSGYFTTLIA
                     RHARSVLALEPVADMQAVLEARCRTDGVDNVQVLGMPASALAEAVPAASVDTAFIIQS
                     LHHLHDRSEVFRALGHVVKPGGRLLMVEPHHNLRRAARLFRKYLTTYRAREFWSDERN
                     WATHDFLTRGELRALCRQGGFDRPRFSGYWIPYSRRFVPDAERRFQLEGRLGRWPVFR
                     HWASVLAVEARRRPEPRS"
     gene            1314..>2079
                     /locus_tag="VNU03_22005"
     CDS             1314..>2079
                     /locus_tag="VNU03_22005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP77213.1"
                     /translation="MSLPAVSVLMGVRDGAPWVAEAIESILGQTLADLELIVVDDGST
                     DDTPTLLAGMRDPRLVVEWQPPKGLTRALGRAFARARAPLVARLDADDLARPDRLERQ
                     HAFLTAHPEVGLLGSGARVVDEAGREVGAIRPPEDDATLRRTLIRRNPFVHSSIMARW
                     KLVAQVGGYDRAFPVAQDYDLWLRLSALTQMANLPEALVTRRLVAGRVTAARDDDRLR
                     SEARARWRAVCRGIYPPWSAVWAVRPLLALAVPRPVR"
BASE COUNT          239 a          780 c          751 g          309 t
ORIGIN      
        1 cgagtactat cgtctcctgg ccccgggcgg gcacatcgcg atcctcgaca ccccgaaccg
       61 gctctttccg ctcgagaccc actcgatcgg cctgccgctc gtgcagtggc tgccgccgcg
      121 gctggcccac gcctacgcgc gcgtggcccg gccccggaag ttcgcgcgcg tgtccttcga
      181 gacgttcgtg gccgacggca ccggctggcg caacgcgacc ctgtccgact gcctgccctc
      241 gcgcgggccg gtgggcctcg aggacgtgac cgaggcggcg ggctacggct ggcgcttctt
      301 ccgcgacacc gcgcgctcgc gggtgcgccg cgctctgctc ccgctctttg gcgccgcggc
      361 gggcgcgctc cgggccgcgg gccggccgcc gtcgctctgc ctgccctatc tgaacctgct
      421 cctgcgcaag cagtgatccg catcgagatc ggccggcgcc actatctgct gtatctgctc
      481 ctgccgcacc cgctcaagtc ccgcctcctg accgcgggct cccgggcgaa gctcgagcgc
      541 atcaaccgga gcttcaacga caccatctac tcggggacgg gcgcgagcgc ctacgacgag
      601 attcatcggt acgcggaaga cgagcagcac gagtacccgg cgcggcccct ggtcgaccgg
      661 gtctggcccg gcgcggcggg ctacgggctg gccctcgagg tgggggccgg cagtggctat
      721 ttcacgaccc tgatcgcgcg ccacgcccgg agcgtgctcg cgctggagcc ggtcgccgac
      781 atgcaggcgg tgctcgaggc ccggtgccgg accgacggcg tcgacaacgt ccaggtgctc
      841 ggcatgcccg cctcggcgct ggccgaggcg gtgccggccg cctcggtcga caccgcgttc
      901 atcatccaga gcctccacca cctgcatgac cggtcggagg tgttccgggc gctcgggcac
      961 gtcgtgaagc cgggcgggcg cctgctcatg gtcgagcccc accacaatct gcgtcgggcc
     1021 gcgcgcctct tccggaagta tctgaccacc taccgcgcgc gggagttctg gtccgacgag
     1081 cgcaactggg cgacccacga ctttctgacg cggggagagc tgagggcgct ctgccggcag
     1141 ggcggcttcg accgcccgcg attctccggc tactggattc cgtactcgcg ccgcttcgtt
     1201 ccggacgccg agcgccggtt ccagctcgag ggaaggctgg ggcgctggcc ggtcttccgt
     1261 cactgggctt cggtcctggc cgtcgaagcc cgccgccggc ccgagccccg atcgtgagcc
     1321 ttcccgcggt gtcggtgctc atgggcgtgc gtgacggcgc gccgtgggtc gccgaggcga
     1381 tcgagagcat cctcggccag accctggccg acctcgagct gatcgtggtg gacgacggct
     1441 cgacggacga caccccgacg ctcctggccg ggatgcgcga tccgcggctc gtggtcgagt
     1501 ggcagccgcc gaagggtctc acgcgcgcgc tcggccgggc cttcgcgcgg gcccgcgccc
     1561 cgctggtggc ccggctcgac gccgacgacc tggcgcgtcc cgatcggctc gagcggcagc
     1621 acgcgtttct caccgcccac ccggaggtcg gcctcctcgg cagcggcgca cgcgtggtgg
     1681 acgaggccgg ccgcgaggtg ggggcgatcc ggccgcccga ggacgacgcg accctgcgcc
     1741 gcacgctgat ccggcgcaat ccgttcgtcc actcctcgat catggcgcgc tggaagctgg
     1801 tcgcgcaggt gggcgggtac gatcgggcct ttccggtcgc gcaggactac gatctctggc
     1861 tgcggctcag cgctctcacc cagatggcca acctgcccga ggcgctggtg acccgacggc
     1921 tcgtggcggg gcgggtgacc gcggcgcgcg acgacgaccg gctgcgctcg gaggcccggg
     1981 cgcgctggcg cgcggtgtgc cgcgggatct atccgccctg gagcgcggtc tgggcggtac
     2041 gcccgctgct ggccctggcg gtgccgcgcc cggtgcgcg
//
LOCUS       DATMCV010000963         2010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_50891, whole genome shotgun sequence.
ACCESSION   DATMCV010000963 DATMCV010000000
VERSION     DATMCV010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50891"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VNU03_22010"
     CDS             complement(<1..1680)
                     /locus_tag="VNU03_22010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HWP77214.1"
                     /translation="MKLIGMDVGSTTVKAAVVDWRAAAEGEASGYKVVWQDYQRHNTR
                     QAEKVLEFLGRMEDEAQVTPGRDRVFFTGSGAGLLGPLAGAKMIQEVVAVAACVEKLH
                     PDVRFVSEIGGEDMKTIFFTPTGTGRTKQVYMQSACSGGTGTFIEKTARKLQVATETL
                     AGLPYAGMSLHKVSSKCGIFAETDANTLVKTGVPVEEIIASLFEAVVYQNLATLTKGN
                     TPSPEVLLLGGPNLFFRGLQEAWRHHLAKLWEQRKIVLPDGRDAASCITVPAEALYYA
                     CLGCVEIGAGEPEGVAVYEGRDRLRWWVEEGQQEQKAKAGGRALVACAADLQSFVAEY
                     DLKRSATARAAAETAGPVLIGCDFGSTTAKAVVVSPERELLFTCYALSKGNPIEDAQS
                     LFRQVREAGYGNVGGLALTGYGKDLLRDVVGADVAVVETVAHATGTLHFHPDADVICD
                     VGGTDVKIMILRQGTVADFRLNSQCSSGNGAFLQGVAERYSIALEDYAEKAFAAKAMP
                     TLAMGCGVFLQSDIVNQQRKGWAAEEIMAALAAVLPVNVWIYAGQLQNLRAV"
     gene            complement(1680..>2010)
                     /locus_tag="VNU03_22015"
     CDS             complement(1680..>2010)
                     /locus_tag="VNU03_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77215.1"
                     /translation="YSCMPNTMSIGAMAGVIGKHPDLLYAPLEIKGDAEVHALSRCQM
                     ILTEAKKRAQREFDEALAASGMTIEEVRAYLDAHPKMKRATYRVPHMGCAGQAANLVL
                     HVAQKAG"
BASE COUNT          276 a          729 c          674 g          331 t
ORIGIN      
        1 gaccgcgcgg aggttctgga gctggcccgc gtagatccat acgttcaccg gcagcaccgc
       61 ggcgagcgcg gccatgatct cctcggcggc ccaccccttg cgctgctggt tgacgatgtc
      121 ggactggagg aacaccccgc agcccatcgc gagcgtgggc atcgccttgg ccgcgaaggc
      181 cttctcggcg tagtcctcga gcgcgatcga gtagcgctcg gccaccccct gcaggaacgc
      241 cccgttgccc gaggagcact gcgagttgag gcggaagtcg gccaccgtgc cctgccgcag
      301 gatcatgatc ttcacgtcgg tgccgcccac gtcgcagatc acgtcggcgt cgggatggaa
      361 gtggagcgtg ccggtggcgt gggccaccgt ctccaccacc gccacgtcgg cgccgaccac
      421 gtcccgcagg agatccttgc cgtagccggt cagcgccagg ccgccgacgt tgccgtagcc
      481 cgcctcacgc acctggcgga agagcgactg ggcgtcctcg atgggattgc ccttcgacaa
      541 cgcgtagcag gtgaagagca gctcgcgctc gggggagacc accaccgcct tggcggtggt
      601 gctgccgaag tcgcagccga tcagcaccgg gcccgcggtc tccgcggcgg cgcgcgcggt
      661 cgcggagcgc ttcagatcgt actcggccac gaacgactgg aggtcggcgg cgcaggccac
      721 cagcgcgcgc ccgcccgcct tggccttctg ctcctgctgg ccctcctcca cccaccagcg
      781 taggcggtcg cgcccctcgt agaccgcgac gccctccggc tcccccgccc cgatctcgac
      841 gcagccgagg caggcgtagt agagggcctc ggcgggcacc gtgatgcagg aggccgcgtc
      901 gcgcccgtcc ggcagcacga tcttgcgctg ctcccacagc ttggcgaggt ggtggcgcca
      961 ggcctcctgc aggccgcgga agaagaggtt cgggccgccc agcagcagca cctcgggcga
     1021 cggagtgttg cccttggtga gcgtggccag gttctggtag accaccgcct cgaagaggct
     1081 cgcgatgatc tcctcgaccg gcacccccgt cttcacgagc gtgttggcgt cggtctccgc
     1141 gaagatgccg cacttgctgc tgaccttgtg caggctcatg cccgcgtagg gcagcccggc
     1201 cagggtttcg gtcgccacct ggagcttgcg cgcggtcttc tcgatgaagg tgccggtgcc
     1261 gccgctgcag gccgactgca tgtagacctg cttcgtgcgg ccggtgccgg tgggcgtgaa
     1321 gaagatcgtc ttcatgtcct cgccgccgat ctctgacacg aagcgcacgt ccgggtggag
     1381 cttctccacg caggccgcga ccgccaccac ctcctggatc atcttggccc cggccagtgg
     1441 ccccagcagc cccgcgccgg atccggtgaa gaagacccgg tcgcgccccg gcgtcacctg
     1501 cgcctcgtcc tccatgcggc cgaggaactc cagcaccttc tcggcctggc gggtgttgtg
     1561 ccgctggtag tcctgccaga cgactttgta tccgctcgcc tcgccttcgg cggcggctcg
     1621 ccaatcaact accgccgctt tgaccgtggt ggagccgacg tccatcccga tgagcttcat
     1681 cacccggcct tctgcgccac gtgcagcacc aggttggcgg cctgcccggc gcagcccatg
     1741 tgcggcacgc ggtaggtcgc gcgcttcatc ttcgggtgcg cgtcgaggta ggcgcgcacc
     1801 tcctcgatcg tcatcccgct cgcggcgagc gcctcgtcga actcgcgctg ggcccgcttc
     1861 ttggcctcgg tcaggatcat ctggcagcgc gagagcgcgt gcacctccgc gtcacccttg
     1921 atctcgagcg gggcgtagag caggtccggg tgcttgccga tcacgccggc catcgccccg
     1981 atggacatgg tgttgggcat gcaggagtag
//
LOCUS       DATMCV010000964         3816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_519050, whole genome shotgun sequence.
ACCESSION   DATMCV010000964 DATMCV010000000
VERSION     DATMCV010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519050"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VNU03_22020"
     CDS             complement(<1..989)
                     /locus_tag="VNU03_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HWP77216.1"
                     /translation="MKIVVTARSGAGEFECEPGEKILHAGLRSGLELPYECGTGTCGT
                     CKARLVSGRVESEWPEAPGNRHLKDPADLLMCQCVAREACALEVSVLRPREAGAAPPG
                     ARRGRVSGWRRLTHDVAAFDVDLEHPLAFAAGQFALLTVPGIAGARAYSMVNFEPGTR
                     RLSFVVKRKPGGAVSDWVFGDGVDGTPLGVFAPLGHATFQPGLGKHVLCIAGGSGIAG
                     MMSILQCAAEADHFAGWDGHVFFGVRSERDHFFLDELHRLRARHPARLGITIALSDED
                     VPASLPAAYPGLAFARGLVHAVAGERMTGRFADVRAYVAGPPPMVDASLRLLLR"
     gene            complement(997..1839)
                     /locus_tag="VNU03_22025"
     CDS             complement(997..1839)
                     /locus_tag="VNU03_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWP77217.1"
                     /translation="MAQMPIEVRPITGALGAEIHGVDLGADLDAASVAAVRRALLDHC
                     VVFFRDQRLDAAQQKALARRFGEIFVHPNYATVVADPEIIEIRREPGDVRIVGEEWHT
                     DTTMMAEPPMGAILYAIDVPPYGGDTLFASQYLAYEALSDGMKRLLEGLRAVHSDRKV
                     AGPQAGMNAYRATKVREEGWEETVSTHPVVRTHPETGRKLLFVNHSYTLRFDGMTEAE
                     SAPLLGYLLEHGHRPEFTCRFRWANGSVAFWDNRCVKHLAVHDAGRFRRIMRRVQIAG
                     DKPV"
     gene            complement(1863..2510)
                     /locus_tag="VNU03_22030"
     CDS             complement(1863..2510)
                     /locus_tag="VNU03_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWP77218.1"
                     /translation="MSGANGATEFTDVVGTLDELRALMGEPSEIALRKDIGRLDEHCR
                     AFIARAPFVLVATADRAGRCDVSPKGDAPGFVQVVDDRTLLVPDRPGNKRFDGMRNLL
                     ENPGIGLLFLVPGSEETLRVNGRARIVRDAVWLARLAAQGKTPQLAIAVDVEESFLHC
                     AKCVKRSGLWETGRWPDRTGLASPAQMFRDHARLNTMTVEELDQRLQDGYRKNLY"
     gene            complement(2667..3368)
                     /locus_tag="VNU03_22035"
     CDS             complement(2667..3368)
                     /locus_tag="VNU03_22035"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77219.1"
                     /translation="MNPLTPRRLIVYATLVACLGSANLADAGAPTEMLRGVFNDANRV
                     LTDPTTEHRPLDRLIAIRGLFSRVFDFRDAAERSLGRQWQARTAAEQQEFTRLFADFV
                     QRGFVYWLASVAEVDGHAGGATVQFVSESVDHDIATVQTQILARGGRLIPLTHTLAYR
                     NRRWVVRDVSIEGISLVGSYRSQFDRVIRGSSYPQLLQLIRARIANEYPFPEPAGPGA
                     AAIYTPARDIPFETR"
     gene            complement(3624..>3816)
                     /locus_tag="VNU03_22040"
     CDS             complement(3624..>3816)
                     /locus_tag="VNU03_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HWP77220.1"
                     /translation="ADSGAYWRQAAVYADKILRGALPATLPVEQPTTFELVVNLRTAR
                     TLGLTLPPSLLLRADEVIE"
BASE COUNT          550 a         1325 c         1402 g          539 t
ORIGIN      
        1 cgcagcagca aacgcaagct cgcgtccacc atcgggggcg ggcccgccac gtaggcgcgg
       61 acgtcggcga agcggcccgt catgcgctcg ccggcgaccg cgtggacgag cccgcgcgcg
      121 aacgcgagcc cgggataggc cgcgggcagc gacgctggca cgtcctcgtc cgacagcgcg
      181 atcgtgatcc cgagccgcgc cgggtggcgc gcccgcagcc ggtgcagctc gtcgaggaag
      241 aagtggtcgc gctcggagcg gacgccgaag aagacgtggc cgtcccaccc cgcgaagtgg
      301 tcggcctcgg cggcgcactg gaggatcgac atcatgcccg cgatgccgct gccgcccgcg
      361 atgcacagca cgtgcttgcc gaggccgggc tggaaggtcg cgtgccctag gggcgcgaag
      421 accccgagcg gcgtgccgtc gaccccgtcg ccgaagaccc agtcgctcac tgccccaccg
      481 ggcttccgct tcaccacgaa cgacagccgg cgggtccccg gctcgaagtt caccatcgag
      541 taggcgcggg cccccgcgat acccgggacg gtgaggaggg cgaactggcc cgcggcgaac
      601 gcgagtgggt gctcgagatc gacgtcgaac gcggccacat cgtgggtcag ccgacgccag
      661 ccgctcaccc gccctcgccg ggcgccgggg ggcgcggcgc ccgcctcacg gggcctcagc
      721 acggagacct cgagcgcgca ggcctcgcgc gccacgcact ggcacatcaa gagatccgcc
      781 gggtccttga ggtggcggtt ccccggagcc tcgggccact cgctctccac ccggccgctc
      841 acgagccggg ccttgcacgt gccgcaggtg ccggtgccgc actcgtacgg cagctccagg
      901 ccgctccgga ggccggcgtg gaggatcttc tccccgggct cgcactcgaa ctcgcccgcc
      961 ccgctccggg cggtgacgac gatcttcacc ggcggctcag acgggcttgt cgcccgcgat
     1021 ctggacgcgg cgcatgatgc gccggaaccg cccggcgtcg tgcacggcga ggtgcttgac
     1081 gcaccggttg tcccagaagg cgaccgaccc gttggcccag cggaagcggc aggtgaactc
     1141 gggccggtgg ccgtgctcga gcaggtaccc gagcaggggc gcgctctcgg cctccgtcat
     1201 gccgtcgaag cggagggtgt aggagtggtt gacgaagagc agcttgcgcc cggtctccgg
     1261 atgcgtgcgt acgacggggt gggtgctcac cgtctcctcc cagccttcct cgcgcacctt
     1321 cgtcgcgcgg taggcgttca tccccgcctg gggcccggcc accttgcgat cgctgtggac
     1381 ggcgcgcagg ccctccagca ggcgcttcat gccgtcggag agcgcctcgt aggcgaggta
     1441 ctggctggcg aagagcgtgt cgccgccgta gggcggcacg tcgatggcgt agaggatggc
     1501 gcccatcggg ggctccgcca tcatggtggt gtcggtgtgc cactcctcgc cgacgattcg
     1561 cacatcgccc ggctcccggc ggatctcgat gatctccgga tcggcgacca cggtcgcgta
     1621 gttggggtgg acgaagatct cgccgaagcg gcgcgcgagg gccttctgct gcgcggcgtc
     1681 gagtcgctgg tcgcgaaaga agacgacgca gtggtcgagg agggcccgcc ggaccgccgc
     1741 cacgctcgcc gcgtcgaggt cggcccccag gtcgacgccg tggatctcgg cgcccagcgc
     1801 accggtgatg ggacggacct cgatcggcat ctgagccatg tctgttctcc cggggcggct
     1861 catcagtaca ggttcttgcg gtagccgtcc tgcaggcgct ggtcgagctc ctcgacggtc
     1921 atggtgttga gccgggcgtg gtcgcggaac atctgggccg gcgaggccag gcccgtgcgg
     1981 tccggccagc ggccggtctc ccagaggccg gagcgcttca cgcacttggc gcagtggagg
     2041 aaggactcct ccacgtccac cgcgatcgcg agctgcgggg tcttgccctg ggcggccagg
     2101 cgcgccagcc acaccgcgtc ccggacgatc cgggcgcggc cgttgacccg cagggtctcc
     2161 tcgctgcccg gcaccaggaa gaggaggccg atgccggggt tctcgagcag gttccgcatc
     2221 ccgtcgaagc gcttgttgcc cggacggtcc ggaaccagca gggtccggtc gtccaccacc
     2281 tgcacgaagc cgggcgcgtc gcccttcggg ctcacgtcgc agcggcccgc gcggtcggcg
     2341 gtggcgacga gcacgaaggg cgcccgcgcg atgaaggcgc ggcaatgctc gtcgagccgg
     2401 ccgatgtcct tgcggagcgc gatctcggag ggctcgccca tcagcgcgcg aagctcgtcg
     2461 agggtcccca ccacgtccgt gaattccgtc gccccgttcg ctccgctcat gcgctacctc
     2521 ccgggaaggt ctctggtgta cccgaagata ccattcaggc gggagttctt cccatccgag
     2581 gagggcggcg gcggatcaag acagaatcgg gcgaggcctc ggcgggatcg cgccgtggca
     2641 cgatcccgcg ggctcgggag cccggatcag cgagtctcga acgggatgtc gcgcgcgggg
     2701 gtgtagatgg cggcggcgcc cgggcccgca ggctccggga acgggtactc gttggcgatg
     2761 cgggcccgga tcagctggag aagctggggg taggaggagc cccggatcac ccggtcgaac
     2821 tgggagcggt agctgcccac caggctgatg ccctcgatgc tgacgtcccg gaccacccag
     2881 cgccgattgc ggtaggccag ggtgtgggtg agcgggatca gccgcccgcc ccgggccagg
     2941 atctgggtct gcaccgtcgc gatgtcgtgg tccaccgact cgctcacgaa ctgcacggtg
     3001 gcgccgccgg cgtgcccatc gacctcggcc accgacgcca gccagtagac gaagccgcgc
     3061 tggacgaagt cggcgaagag gcgggtgaac tcctgctgct cggccgcggt ccgcgcctgc
     3121 cactgccggc ccagcgagcg ctcggccgcg tcacggaagt cgaacacccg gctgaagagc
     3181 ccgcggatgg cgatcaagcg gtcgagcggc cggtgctccg tggtgggatc ggtgagcacc
     3241 cggttggcgt cgttgaagac gccacgcaac atttccgtgg gcgcccccgc gtcggcaaga
     3301 tttgccgacc cgagacacgc caccagcgtc gcgtacacga tcagtcgtcg aggggtcaaa
     3361 gggttcatgt tcggccggtt cccaggagtt gcatcgcctg tgccacggcc tcggcggggt
     3421 ttgcgggcgg aagatcgaca tccaggagcg gaacgcgagg gtcggtgcgg tatgagcgcg
     3481 tcttttccga cacagagggt gtaccgaata gcgacgccgg gccgccggcg ccccgacccg
     3541 gccgcgcgca gattctgggg agtagcggtc ctaccgcgag ggccgattcc cggacgcgct
     3601 gcggcgcgag ttctcgctct ggcctactcg atcacctcgt ccgcccgcag caggagcgac
     3661 ggtggaaggg tgaggccgag cgtcctggcg gtcctgaggt tcactaccag ctcgaacgtg
     3721 gtcggctgct cgaccggcag ggtcgccggg agcgctcccc tcaagatctt gtcggcgtag
     3781 accgcggcct ggcgccagta ggcgccggag tccgcg
//
LOCUS       DATMCV010000965         3163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_519069, whole genome shotgun sequence.
ACCESSION   DATMCV010000965 DATMCV010000000
VERSION     DATMCV010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519069"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VNU03_22045"
     CDS             complement(<1..235)
                     /locus_tag="VNU03_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77221.1"
                     /translation="MKSRCLKILVAIALGAGALLTAGALPAPAAPVTVRYLEGVTHGF
                     LVLRGPGGATLAEGDLLQVVRPEGVDSRLIFRFR"
     gene            404..1222
                     /locus_tag="VNU03_22050"
     CDS             404..1222
                     /locus_tag="VNU03_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWP77222.1"
                     /translation="MTRSIRVAAVGDLHCTRASEGTLQPIFAKVAESADVLVLCGDLT
                     DYGTAEEARVLAKELSATKVPTLAVLGNHDFESGGAPEVTQILADAAGVTMLDGTATE
                     VLGVGFAGVKGFAGGFGESALQAWGEEPIKAFVRAALEEALKLESALARLRTPGRVAL
                     LHYAPIRETAGGESAEIFPFLGSSRLEEPLNRYRPSVVFHGHAHRGQPEGATAAGVPV
                     YNVALPLLRRHYPEQPPFRVVLVPVPDEAPAGPRPSATLTRPGVPAEPARPVSA"
     gene            1219..2100
                     /locus_tag="VNU03_22055"
     CDS             1219..2100
                     /locus_tag="VNU03_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HWP77223.1"
                     /translation="MSPAAFPAAPGRARVRSDGPSAVNGRFYRRVLAELNAAGVEFLV
                     GGGHALECYLGIGRSVKDLDLFIRERDVASALRLVEERLGYESEVTFPHWLAKIKRSE
                     HDHIDVIFNSGNGVCAVDDDWFRHAVPSTVMGEPVRLCPAEETIWSKAFVMERERYDG
                     ADIAHLLHARAEALDWGRLARRFGRHWRVLLSHLVLFGFIYPSERTRIPEPLMRRCLH
                     LLEEEMRQAPARRICRGPITSRAQYLVDVQLWGYEDARLLPHGSMTESEASIWTAAID
                     EAASRPVGADALGEAGA"
     gene            2115..2828
                     /locus_tag="VNU03_22060"
     CDS             2115..2828
                     /locus_tag="VNU03_22060"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWP77224.1"
                     /translation="MPALLARRPAAGRRATPAGRSGFWPLAVAGAVLLAMIVVGHTVD
                     VARYLEAGRSWAGSLGVLAPVAFVAVYVVATLIGVPGMPFTLLSPFLFGAWPAVAVMI
                     VASGLSAAAAFLIARYLARDAFVARLTGTPSFARLSALLEAHDWVVIPLLRILPLAPF
                     AVVNYGFGLTAIAFTRYLTWSLVGMIPMNVVLVLGADVFYTAATRGTVPWPLVAGATA
                     AALLLAGLTAAGRRVFARS"
     gene            2833..>3163
                     /locus_tag="VNU03_22065"
     CDS             2833..>3163
                     /locus_tag="VNU03_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77225.1"
                     /translation="MESVVLRVQAMVDRCSRWMRLAPPLGETSRRRFLIVQIDGLSRP
                     LLDRALAGGSLRGLRGLLASGRLARRDLSVGLPSSTPAFQAAIMYGGRPDIPGFHFYD
                     KRAGRELH"
BASE COUNT          366 a         1171 c         1154 g          472 t
ORIGIN      
        1 cgcggaagcg gaagatcaac cgcgagtcca cgccctccgg ccggaccacc tgcagcagat
       61 cgccttccgc gagggtcgcg ccgcccgggc cgcgcaacac caggaagccg tgggtcacgc
      121 cctcgagata gcgcaccgtc accggcgccg cgggcgcggg gagcgcgccg gcggtgagca
      181 gcgcgcccgc accgagcgcg atggcgacga ggatcttcag gcaccggctc ttcatgtact
      241 ggcaagttgc aagcgcgctg ccatcgccgc gccggtctcg cggcgatgag aaagttcccg
      301 gtaagaggtt ccggcgggtc ccgcggccgg gccgcgtcgt ggcaaaaccc gcggttgagg
      361 gcgcgctacg tgcggcatgg gggttgcacg attcaagcgc cttatgactc gctcgatccg
      421 cgtcgccgcc gtcggtgatc tgcactgcac ccgtgcgtcc gaaggcacgc tccagccgat
      481 cttcgccaag gtcgcggaga gcgcggacgt gctcgtcctc tgcggcgacc tcaccgacta
      541 cgggaccgcc gaggaggcgc gggtgctcgc caaggagctc tccgcgacca aggtgccgac
      601 gctcgcggtg ctcggcaacc acgacttcga gtccggcggg gcgccggagg tcacgcagat
      661 cctggccgac gccgcgggcg tgaccatgct cgacggcacc gcgaccgagg tgctgggagt
      721 gggcttcgcg ggcgtgaagg gcttcgcggg cggcttcggc gagagcgcgc tccaggcctg
      781 gggcgaggag ccgatcaagg ccttcgtgcg ggccgccctc gaggaggcgc tcaagctcga
      841 gtcggcgctc gcgcgcctgc gcacgcccgg tcgcgtggcc ctcctgcact acgcgccgat
      901 ccgcgagacg gcgggcggcg agtcggcgga gatctttccg ttcctcggct cgagccggct
      961 cgaggagccg ctcaaccgct accggccgag cgtggtgttc cacggccacg cgcatcgtgg
     1021 gcagcccgag ggggcgaccg cggcgggggt gccagtctac aacgtggcgc tgcccctcct
     1081 gcgccgccac tacccggagc agccgccgtt ccgcgtggtc ctagtgcccg tgccggacga
     1141 agcgcccgcc gggccgcgac cgagcgccac cctcacgcgc cccggagtgc ccgccgagcc
     1201 ggcgcgcccg gtgtcggcat gagccccgcc gcgttcccgg cggcgccggg tcgggcccgg
     1261 gtgcgatcgg acgggccttc cgcggtcaac ggccgcttct accgccgcgt cctcgcggag
     1321 ctgaacgcgg ccggcgtcga gttcctggtg ggcggggggc acgcgctcga gtgctacctc
     1381 gggatcgggc ggtcggtgaa ggacctcgac ctgttcatcc gcgagcgcga cgtggcttca
     1441 gccctccgcc tcgtcgagga gcgcctgggc tacgagtcgg aggtcacgtt cccgcactgg
     1501 ctcgccaaga tcaagcgctc cgagcacgac cacatcgacg tcatcttcaa ctccggcaac
     1561 ggggtgtgcg cggtggacga cgactggttc cggcacgcgg tgccgtccac cgtgatggga
     1621 gagccggtgc ggctctgccc ggccgaggag acgatctggt cgaaggcctt cgtcatggag
     1681 cgcgagcgct acgacggcgc cgacatcgcg cacctgctgc acgcccgcgc ggaggccctc
     1741 gactggggcc ggctcgcgcg gcgcttcggg cggcactggc gcgtgctgct gagccacctc
     1801 gtcctcttcg gcttcatcta tccgtccgag cgcacccgca tcccggagcc gctcatgcgg
     1861 cggtgtctcc atctgctgga ggaggagatg cggcaggcac cggcgcgtcg gatctgtcgc
     1921 gggccgatca cctcgcgcgc ccagtacctg gtggacgtcc agctctgggg ctacgaggac
     1981 gcccggctcc tgccgcacgg cagcatgacc gagagcgagg cctccatctg gacggccgcc
     2041 atcgacgagg cggcgagccg gccggtgggc gccgacgccc tcggtgaggc cggggcctga
     2101 gggaagggga tcccgtgcct gcactcctcg cgcggcggcc cgcggcgggg cggcgtgcca
     2161 ctccggcggg ccgctccggc ttctggccgc tcgccgtcgc gggggcggtc ctcctcgcga
     2221 tgatcgtggt gggccacacc gtcgacgtgg cgcggtatct cgaggcgggg cggagctggg
     2281 ccggctcgct cggcgtgctg gccccggtgg ccttcgtcgc ggtgtacgtg gtggccacct
     2341 tgatcggcgt gccgggcatg ccgttcacgc tgctgtcgcc gttcctcttc ggggcgtggc
     2401 ccgcggtggc ggtcatgatc gtggcgagcg gcctctcggc ggcggccgcg ttcctgatcg
     2461 cgcgctacct ggcccgcgac gcgttcgtgg cgcgtctcac cggcaccccg agcttcgcgc
     2521 gcctctccgc gttgctcgag gcgcacgact gggtggtgat ccccctgctg cggatcctgc
     2581 cgctcgcccc gttcgcggtc gtcaactacg gcttcggcct gaccgcgatc gcgttcacgc
     2641 ggtacctcac ctggtcgctc gtgggcatga tccccatgaa cgtggtgctc gtcctcggcg
     2701 ccgacgtctt ctacaccgcg gccacccgcg gcaccgtgcc ctggccgctc gtggccggcg
     2761 cgaccgcggc cgccctcctg ctcgcgggcc tgaccgcggc cggccgtcgg gtcttcgcgc
     2821 ggtcgtgacg ccatggaatc ggtggtcctc cgggtgcagg cgatggtcga ccggtgcagt
     2881 cgctggatgc ggctcgcccc gccgctcggg gagacgtctc gccgccgctt cctgatcgtc
     2941 cagatcgacg ggctctcccg ccccctcctg gaccgcgcgc tcgcgggcgg cagcctgcgc
     3001 ggcctccgcg gcctcctagc ctcggggcgg ctcgcccgcc gcgacctgtc ggtggggctg
     3061 ccctcgtcga cccccgcctt ccaggccgcc atcatgtacg gcgggcgacc cgacattccc
     3121 ggcttccact tctacgacaa gcgggccggc cgggagctgc act
//
LOCUS       DATMCV010000966         2478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_716823, whole genome shotgun sequence.
ACCESSION   DATMCV010000966 DATMCV010000000
VERSION     DATMCV010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716823"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1986
                     /locus_tag="VNU03_22070"
     CDS             199..1986
                     /locus_tag="VNU03_22070"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWP77226.1"
                     /translation="MVRPTGAIFVHGVDMARHTGCFRCSWLLLVSALLLSPAPALAQP
                     TELRTVTLRAAPEEMYRAQINWEATLRQTVKTVSAIYEKNFQIRLVIQDIVPWTVGPS
                     VPINRMLTRLRAEVPTGPADIVVAFAAERCEKLQYGVAWGLERYALVQSNCLDTAVLS
                     NTTPEAALTHEIAHLFGAFHPAAGPVSVMRAGPADRFDDQTVRVIRLMRTFDFARGVL
                     GLDADTRRAWSAIYAEGHARDEPNLLAEALATAGWREVTSGQVAEGEARIREAMAIDP
                     RAPHPHVLLGHLFMQQQRLEDAVRELRTATRLNFRLVEARVQLGFLLRKLNRDDEALV
                     EFRDAVRIDPRAGRAYVGIGMILADRDRLPEAIDVLRSATQMTPTEGSAFLQLATVLA
                     KSGRPDEAGAAAERAQKLGQAVPPALLPKRPDPVVVAAPAPGPRRPPSVGVSTPASAP
                     RSQDPTTILATCPGPANPGADPPVSLEDDPPRFREYFAVLRTRITARWTYPRSAGERN
                     IEGDLLIEFHIRKDGRLDCAVVRRSSGTTILDEAALDAVRLAAPYPSIPDDVAKQTLV
                     INCTFRYQIVSGLSNAPSQTNPNPTAPKP"
     gene            complement(1997..>2478)
                     /locus_tag="VNU03_22075"
     CDS             complement(1997..>2478)
                     /locus_tag="VNU03_22075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HWP77227.1"
                     /translation="SASDVTEWMSMECSRAWFMKLRAALGDTFLTNQRRPILTLVEDT
                     SGGAHDTLMAACDAPRYRLLGVEGHHDNCRDNLHAALGALGVKVPATPSPLNLFMNIP
                     WTPDGTLAWAEPVSPAGSYAVLRAEMDLVIAFSACPQDVLPINGRTGHTTEAHFSIE"
BASE COUNT          369 a          909 c          822 g          378 t
ORIGIN      
        1 gtctgcggcg ggcggggcct cctcaagcgc tacccgctcg agcgctacgc gcgcgacgcg
       61 cgggcgggcg cgctgatgcg gccgtggacc caggagatcg ccacccagca ggcgtgggaa
      121 gcggcgctcg gcctggacgc ctccgcttga ctcgccgcgt ccgccactcc atagtcctcg
      181 ccaacgaccg acccccccat ggtgcgtccc accggggcga tcttcgtcca cggagtggac
      241 atggctcgtc acaccgggtg cttcagatgt agctggctcc tcctggtctc cgcccttctg
      301 ctctcccccg cgcccgcgct ggcgcagccc accgagctcc gcaccgtcac cctgcgagcc
      361 gcccccgagg agatgtaccg cgcccagatc aactgggagg ccacccttcg ccagaccgtc
      421 aagaccgtct ccgccatcta cgagaagaat ttccagattc ggctggtgat ccaggacatc
      481 gtgccgtgga cggtgggtcc ctcggttccg atcaaccgca tgctgacccg cctgcgggcg
      541 gaggtgccga ctgggccggc cgacatcgta gtggcgttcg cggccgagcg ctgcgagaag
      601 ctccagtacg gggtggcctg gggactcgag cgctacgccc tcgtccagtc gaactgtctc
      661 gacaccgcgg tgctctccaa tacgacgccc gaagccgcgc tcactcacga gatcgctcat
      721 ctattcggtg ccttccatcc ggccgcgggg ccggtctcgg tgatgcgggc cggcccggcc
      781 gatcgcttcg acgatcagac cgtccgggtc atccgcctca tgcgcacctt cgacttcgcg
      841 cgcggggtgc tgggactcga tgccgacacc cgccgggcgt ggagcgccat ctacgcggag
      901 ggtcacgcgc gcgacgagcc gaatctgttg gccgaggcgc tcgcgaccgc gggatggcgc
      961 gaggtgacgt ccggccaggt cgccgagggc gaggctcgga tccgggaagc catggcgatc
     1021 gacccaaggg cgcctcaccc tcacgtgctg ctaggccatc tctttatgca gcagcaacgg
     1081 ctcgaggacg cggtccgcga gctgcgaacc gcgacccgcc tgaacttccg tctggtcgaa
     1141 gcccgcgtcc agctcggctt cctgctgcga aagctcaacc gtgacgatga agccctcgtc
     1201 gagtttcgcg acgccgtgcg catcgatcct cgcgcgggcc gcgcctacgt cgggatcggc
     1261 atgatcctcg ccgaccgcga ccggctgccc gaggcgatcg acgtgctgcg gagcgccacc
     1321 cagatgacgc ccaccgaggg ctcggccttt ctgcagctcg ccacggttct cgccaagagc
     1381 ggccgccccg acgaggcggg ggcggccgcg gaacgggccc agaagctcgg gcaagcggtt
     1441 cctcccgcac tgttgccgaa acggccagac cccgtcgtag tcgccgcgcc ggccccaggg
     1501 ccgcgacggc cgccttcggt gggggtgtcc acgccagcgt ccgcacctcg ctcccaggat
     1561 ccgaccacga tcctcgcgac ctgtcccggc ccggccaacc cgggcgccga tccccccgtg
     1621 tccctggaag atgacccgcc gagattccgc gagtacttcg ccgtcctgcg cacgcggatc
     1681 acggcgcgct ggacctatcc ccgctcggcc ggagagcgaa acatcgaggg agatctcctg
     1741 atcgagttcc acatccggaa agacggccgg ctcgattgtg cggtggtgcg tcggtcttcc
     1801 ggcacgacga tcctcgacga ggccgcgctc gatgccgtgc ggctcgccgc cccgtacccg
     1861 tcgattccgg atgacgtcgc caagcagacc ctcgtcatca actgtacgtt ccggtaccag
     1921 atcgtgtcgg gcctgtcgaa cgccccaagc cagacgaatc ccaaccccac ggctccgaag
     1981 ccctgaagcc gcccgactac tcgatgctga agtgcgcctc ggtggtgtgc ccggtgcggc
     2041 cgttgatggg cagcacgtcc tgcggacacg cggagaacgc gatcaccagg tccatctccg
     2101 cgcgcagcac cgcgtagctc cccgcgggtg acacgggctc ggcccaggcg agcgtgccgt
     2161 ccggcgtcca cgggatgttc atgaacaggt tgagcggcga cggggtggcc ggaaccttca
     2221 cgccgagcgc gccgagcgcg gcgtggaggt tgtcgcggca gttgtcgtgg tggccctcca
     2281 ccccgagcag acggtagcgc ggggcgtcgc aagcggccat gagggtgtcg tgggcccctc
     2341 ccgaggtgtc ctccaccagg gtcaggatcg gccggcgctg attggtgagg aacgtgtcgc
     2401 cgagggcggc gcggagcttc atgaaccagg cgcggctgca ctccatcgac atccactcgg
     2461 tcacgtcgct cgcgctga
//
LOCUS       DATMCV010000967         3125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_519166, whole genome shotgun sequence.
ACCESSION   DATMCV010000967 DATMCV010000000
VERSION     DATMCV010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519166"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VNU03_22080"
     CDS             <1..1352
                     /locus_tag="VNU03_22080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HWP77228.1"
                     /translation="RDGLRAGLLVPLCSRGRVLGLLALADVEPREFSPEEVEMALTLA
                     AVTAVALDSRRGLEDLRQALGEQAARQAEQAEGDKARVLTAFGAGLTRELSAVFALLL
                     GKSQLMLARAPDESLREGLAALEEAAWRGTDVTQRLLGLAEADRAADLSCDLGTIVQE
                     ALAFARPRPGSAPAGGRIEMTADLPPTSPVAGSATALREAIVNLVLNAMEAMPAGGSV
                     TVRLREVEHGVELVVADSGAGIAPEVKPRIFDPFFTTRPGHLGLGLTVVQAVVLRAGG
                     RVDVASGPAGTTVTLWLPAAPASGATRSDESLAGEPAPEPALEVPPAPGPVGSVLIVE
                     EEDGIRSAVLDALAAAGHRVEAAVDATSALDRLALGGIDVVVTDLALRDRSGLQLAAA
                     VKQRSPGAAVVLLTGWGRRLHEERVRGAGVDVMVVKPIEPERVRTAVAEALRLHPPA"
     gene            1349..2281
                     /locus_tag="VNU03_22085"
     CDS             1349..2281
                     /locus_tag="VNU03_22085"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HWP77229.1"
                     /translation="MTRRLATVDLGTNTVRLLVVEPDGPAWRTLQQSQRVTRLGEGQG
                     DGGRLGPEPMRRTSATVAEFARAARALGAGRVRIVATSAVREAANREEFVARLERESG
                     ERVEVVSGEEEARLTLKGVTSGLPGLGGSFVLFDIGGGSTEFVLARGGTPAAAVSLRL
                     GVVALQEEWGEPGPVRADHFARLRRHVEQRLAAEVPEAIAGAPAAELVGTAGTVTTLA
                     ALELGLRAYDATAVHGYRLERAAVERQLARLGALGVEARGRLPCLEPGRADVIIPGIA
                     ICLAAMARCGRESLVVSDRGLREGILCELLGTAA"
     gene            2278..>3125
                     /locus_tag="VNU03_22090"
     CDS             2278..>3125
                     /locus_tag="VNU03_22090"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HWP77230.1"
                     /translation="MTAPSARTLSVIDYHTEGEPMRIVVGGVGAVPGATLMERSEYLA
                     AHGRDLLGFTLYEPRGHAAMCGAILTEPVAPEADAGVLFIEPLGPVHMCGHGAIALGA
                     MLVESGRVAAPGGDATVTLETPAGLVACRVRAQAGRPTSVTIRNVPAFSVALDRTVEI
                     AGSGAVRFDLAYGGHFYALVEAAPLGFTLDPKEAPRLVEIGERIRLAIEATVPLVHPA
                     MPAARGLLYVQFYEPARRPDAQYRNAVVVAPAGLDRSPCGTGTSARLANLHARGRLGV
                     GEGFGH"
BASE COUNT          342 a         1140 c         1244 g          399 t
ORIGIN      
        1 agcgcgacgg gctccgggcc gggctcctgg tgccgctctg cagccgcggg cgcgtgctcg
       61 gcctgctggc cctggccgac gtcgagccgc gcgagttctc ccccgaggaa gtggagatgg
      121 cgctcacgct cgccgcggtg accgcggtgg cactcgatag ccggcggggg ctcgaggatc
      181 tacggcaggc cctgggcgag caggccgcac ggcaggccga gcaggcggag ggagacaagg
      241 cgcgcgtgct gaccgccttc ggcgcgggtc tgacccgcga gctgagcgcg gtcttcgcgc
      301 tcctgctcgg caagtcccag ctcatgctcg cgcgggctcc ggacgagtcg ctccgcgagg
      361 ggctggccgc gctcgaggaa gcggcgtggc gcggaaccga cgtcacgcag cggctcctcg
      421 ggctggccga ggccgatcgc gcggcggatc tctcgtgcga cctgggcacg atcgtgcagg
      481 aggcgctcgc cttcgcccgt ccgcggcccg gctcggctcc ggccggtggc cgcatcgaga
      541 tgaccgccga cctgccgccg acgtcgccgg tggccggcag cgcgaccgcg ctccgcgaag
      601 ccatcgtcaa cctcgtcctg aacgcgatgg aggcgatgcc cgcgggcggg agcgtgaccg
      661 tgcggctgcg cgaggtcgag cacggcgtcg agctggtggt cgcggacagc ggcgccggca
      721 tcgcgccgga ggtgaagccg cgcatcttcg atcccttctt cacgacgcgt ccgggacacc
      781 tgggcctcgg gctcaccgtc gtccaggcgg tcgtgctccg cgcgggcggg cgcgtcgacg
      841 tggccagcgg tcccgcgggc accacggtca ccctctggct gcccgcggcg ccggcgagcg
      901 gggcgacgcg gtccgacgaa tccctcgcgg gcgagccggc gccggagccc gctctcgagg
      961 tcccgcccgc gccgggaccg gtcgggtcgg tcctgatcgt cgaggaggag gacggcatcc
     1021 ggagcgcggt actcgacgcg ctggccgcgg ccggccaccg cgtggaggcc gcggtcgacg
     1081 ccaccagcgc gctcgatcgc ctcgccctgg gcggcatcga cgtggtggtc accgacctgg
     1141 cgctgcgcga tcgctcgggc ctgcagctgg ccgccgcggt gaagcagcgg agtcccggcg
     1201 cggcggtggt gctcctcacc ggctggggtc gccggctcca cgaggagcgc gtgcgcgggg
     1261 cgggcgtgga tgtgatggtg gtgaagccga tcgagcccga gcgcgtccgc accgccgtcg
     1321 cggaggcgct ccgcctgcac ccgcccgcgt gacccggcgt ctcgccaccg tcgatctcgg
     1381 caccaatacc gtccggctgc tcgtcgtcga gccggacggc cccgcgtggc gcacgctcca
     1441 gcagagccag cgcgtgaccc ggctcggaga agggcagggg gacggcggac gtctcggccc
     1501 ggagccgatg cgccggacct ccgcgacggt ggccgagttc gcgcgggcgg cgcgcgccct
     1561 cggcgcgggc cgcgtgcgta tcgtggccac cagcgcggtc cgcgaggccg cgaaccgaga
     1621 ggagttcgtg gcccggctcg agcgcgagtc gggcgagcgc gtcgaggtcg tctccggcga
     1681 ggaggaggcg cgcctgaccc tcaagggcgt gacctcggga ctgcccggcc tcggcggctc
     1741 cttcgtgctc ttcgacatcg gcggcgggag caccgagttc gtcctcgcgc ggggcgggac
     1801 gcccgcggcc gcggtgagcc tgcggctcgg cgtggtggcg ctccaggaag aatggggcga
     1861 gccggggccg gtgcgcgccg accatttcgc ccgcctgcgt cggcacgtgg agcagcggct
     1921 cgcggccgag gtgccggagg ccatcgcggg cgcgcccgct gcggagctgg tcggaaccgc
     1981 gggcaccgtc accacgctgg ccgcgctcga gctcgggctc cgcgcctacg acgcgacagc
     2041 cgtccacggc taccgcctcg agcgggccgc ggtggagcgg cagctcgcgc ggctgggcgc
     2101 cctcggcgtg gaggcgcgcg gccgccttcc gtgtctcgag ccgggccgcg ccgacgtgat
     2161 catcccgggc atcgcgatct gcctggccgc gatggcacgc tgcgggcgcg agagcctggt
     2221 ggtgagcgat cgcgggctcc gcgagggcat tctctgcgag ctgctgggga ccgccgcgtg
     2281 accgcgccgt cggcccgcac cctgtcggtg atcgactatc acaccgaggg ggagcccatg
     2341 cgcatcgtgg tggggggcgt cggcgcggtg cccggtgcca ccctgatgga gcggagcgag
     2401 tacttggccg cgcacggccg cgacctgctc ggcttcacgc tctacgagcc gcggggccac
     2461 gccgcgatgt gcggggcgat cctgaccgag ccggtcgcgc cggaggcgga cgcgggcgtg
     2521 ctcttcatcg agcccctcgg gcccgtgcac atgtgcgggc acggggcgat cgcgctcggc
     2581 gcgatgctgg tggagagcgg gcgcgtggcc gccccggggg gcgacgccac cgtgaccctc
     2641 gagacgcccg cgggcctcgt ggcctgccgg gtgcgcgcgc aggccggccg gccgacgtcg
     2701 gtgacgatcc gcaacgtgcc ggccttctcg gtggctctcg accggacggt ggagatcgca
     2761 ggctcgggcg cggtccgctt cgatctcgcc tacggcggcc acttctacgc gctggtggag
     2821 gccgcgccgc tcgggttcac gctcgatccc aaggaggcgc cgcggctcgt ggagatcggc
     2881 gagcggatcc ggctcgccat cgaggccacc gtgccgctcg tgcaccccgc gatgccggcg
     2941 gcccgcggcc tgctgtacgt ccagttctac gagccggcgc ggcggcccga cgcgcagtac
     3001 cggaacgcgg tggtggtggc tcccgcgggc ctcgatcgct cgccctgcgg caccgggacc
     3061 tccgcacggc tcgccaatct ccacgcgcgc ggccgcctcg gggtcgggga ggggttcggg
     3121 cacga
//
LOCUS       DATMCV010000968         7726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_119749, whole genome shotgun sequence.
ACCESSION   DATMCV010000968 DATMCV010000000
VERSION     DATMCV010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119749"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VNU03_22095"
     CDS             complement(<1..764)
                     /locus_tag="VNU03_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77231.1"
                     /translation="MAQATLVKRGAYHDSVTLLTLARALRARPGVREAAVVMGTPANH
                     DVLAQAGLLTEEARTATPNDLVIVVEAETPTGAEAAAAAAEPLLARSAPRGQAERPRP
                     RTTAGAIRRMPEANLALISVPGAFAAVEARAALSASLNVILFSDNVSLADEIALKRQA
                     TARGLLLMGPDCGTAYIADVPLGFANAVPPGRVGLVAASGTGLQQVASLLAGRGRGLS
                     HAIGVGGRDMSEAVGGIMTLAALDLLAANAATELIVV"
     gene            complement(768..1580)
                     /locus_tag="VNU03_22100"
     CDS             complement(768..1580)
                     /locus_tag="VNU03_22100"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HWP77232.1"
                     /translation="MTGRPGDLVRLSAQIARRLEASELEGRVHSVFARAVNIEWSDGS
                     LLALHGPGPLLAPFAAVVDDLTLLSALRPGMEVTAGPHRLRAPGLTLSWARAVRVDCS
                     AAGARPIRARAIPTPPGAHAGHAPGLDSPVGVGARATLAAGIAGRDATRLIAGACALL
                     GLGEGLTPAGDDCLVGALAVLHAVDAGALASEPGPVAPIAAAAAARTTAIGREFVLHA
                     LAGRFSEPVLAVLRARSSLESALAAERLRGLGATSGADTLAGMRLAWDALAA"
     gene            complement(1591..2955)
                     /locus_tag="VNU03_22105"
     CDS             complement(1591..2955)
                     /locus_tag="VNU03_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP77233.1"
                     /translation="MPGEHILIVDDERAIRTTLRGVLEDEGYRVTAVGSGEEAIARIQ
                     EETPDIIFLDIWMPGIDGLDTLAEMKRVRPETAVVMISGHGTIETAVKATKLGAYDFI
                     EKPLSLEKTLLIASRALDHTRLERENRNLRAQLERGQEIVGKSPLIEHLRQQIAIAAP
                     SSGRVLIQGENGSGKELVARAIHALSARRDGPFVEVNCAAIPEELIESELFGHERGAF
                     TGAVARRRGKFELADQGTLFLDEIGDMSLKTQAKVLRALEEQAFERVGGKDTIRVDVR
                     VLAASNQNLPERIAAGGFREDLYYRLNVIPIEVPPLRQRKEDLPALVAHFVTVFSAEN
                     GKRPKTVSVEALAYFLSYDWPGNVRELRNMVERLVIMSPRDVIAPEDLPPPLRPRDAE
                     PPAEDAQRERTLKEAREAFERAFILGELRANEWNMTRTAERLGIERSHLYRKLKAYGI
                     SAPK"
     gene            complement(2970..5213)
                     /locus_tag="VNU03_22110"
     CDS             complement(2970..5213)
                     /locus_tag="VNU03_22110"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77234.1"
                     /translation="MTPLTRRPERPDEQEKRKRNLLIISGILILLVVATVFEVGIRAP
                     KIPFASNLIVFFLFNLNLIVFLLLLVLLFRNLVKLSFERRHKIIGSRFKAKLVLTFLA
                     LSLVPGILIFLIASNFITTSIEGWFKPQVERPLDQAMEVAQTYYQSLESTALRHGRYM
                     AKVVERDGLLAESKREALAGYLTEQQERLGVAAITIFNRDGQELVHVKNPVLAKVPTR
                     STNSEHVKQALGGQEITTVHELDNGDMIQAMVPIRDADQAVSGAMVVAIHVPQRLEAR
                     LRNISQAFQEYKQLRLLKNPIKGIYILLFLLMTLIIVFSATWFGLYLARGITEPIQML
                     AEGTREVAAGNLNYKVEVRADDEIGILVDSFNRMTGDLAASKSKLEETYRDLQAKHGE
                     MEERRRYTETVLEAVATGVVSLDPDGRVTTINGAAERLLGLDAVTVQGRSATRVFRSP
                     EYVEIDALIQRMGRVREGMLDREVHLRRHGQAVALLASATALRGPDGGYVGMVLAFDD
                     LTELLKAQRLAAWREVAQRIAHEIKNPLTPIQLSAERLRRRLASNRSPEEKRLLEEAT
                     GTIIQEVDGLRQLVDEFSRFARMPALTPKPTDLARLLEGVVVLYRESHPSLAIRSVFS
                     ELPPIEVDPDQIKRAVLNLVDNAVEAVGGTGEVVVQTEWLPEAHRARIVVADDGPGIA
                     ADDRDRLFVPYFSTKATGMGLGLPIVHQIVSDHGGTIRVEDNTPHGSRFVIELPAGRP
                     VVAPVQA"
     gene            complement(5210..5806)
                     /locus_tag="VNU03_22115"
     CDS             complement(5210..5806)
                     /locus_tag="VNU03_22115"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HWP77235.1"
                     /translation="MGTRGRPLRRILPGASLLVALLVGLPSGPAPVGAEVRISNLSVF
                     LNDYDVTVTIVLFGAVPDSLHESIHTGIAAHVRYYVELWQYTRMGVDRRLQARMVERQ
                     LTYNVLTKEYKVASLKGETREPFLTKDLREAQRVISELRVGQLAPVAALDKQELYYVR
                     VRSDVSLGGVNSWFARMTGEAEETPWVQSSVLTPVRNQ"
     gene            complement(5920..6840)
                     /gene="lpxC"
                     /locus_tag="VNU03_22120"
     CDS             complement(5920..6840)
                     /gene="lpxC"
                     /locus_tag="VNU03_22120"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HWP77236.1"
                     /translation="MDLQQTLRQPVKLAGIGLHSGEPVEMTVSPAGADTGILFRAADG
                     TLIPANADHVVDTNSATTVGAFGVRVRTIEHLMAAAAALGIDNAVVDIHGPEVPAADG
                     SAKPFMDLLRSGGRASLPAPRRPIVLSEPIRVGTESRWLEVLPAESLRVSYTLDNSHP
                     IIGLQAGTYGITEDVFDQELAPARTYGFLRDVPVMRQNGLARGGSLENAVVVGKRSVL
                     NDSLRYPDEFVRHKILDLVGDLFLLGRPLRALVVGRNAGHALNYQLVSAIQKAVAADR
                     RRSVARVIRPAAPVPVASTGDGFLPGVAAL"
     gene            complement(7000..7404)
                     /locus_tag="VNU03_22125"
     CDS             complement(7000..7404)
                     /locus_tag="VNU03_22125"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HWP77237.1"
                     /translation="MELTATGATLSEAFTRIALGLFAAAVDPDTVGDGDTREVRAHGV
                     GLPALLHAWLQECLYVHEVEGFACRSLEFAVFETAPSAGGEALRLHAILRGEEIDPSR
                     HRVLAAIRNVSRDGLSVESGSKGFSLRASLEI"
     gene            complement(7406..>7726)
                     /locus_tag="VNU03_22130"
     CDS             complement(7406..>7726)
                     /locus_tag="VNU03_22130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HWP77238.1"
                     /translation="GRAAVPEITAPRTLSTWRPDPERDGLLICFWEEEPGGLEGRLPA
                     GPCPAATLIVGPEGGLTAEEVRGLAAAGALVAGLGPRVLRTETAGAVAVALLQSRYGD
                     LGRA"
BASE COUNT         1099 a         2667 c         2850 g         1110 t
ORIGIN      
        1 accacgatca gctcggtggc cgcgttggcc gcgagcaggt cgagcgcggc cagggtcatg
       61 atcccgccga cggcctcgct catgtcgcgc ccgcccacgc cgatggcgtg ggagaggccg
      121 cgcccgcggc cggccagcag cgaggccacc tgctgcagcc cggtgccaga ggcggccacg
      181 agacccacgc gcccgggcgg caccgcgttg gcgaagccga gcgggacgtc ggcgatgtac
      241 gcggtgccgc agtccgggcc catcaggagg agccctcgcg cggtggcctg gcgcttgagc
      301 gcgatctcgt cggcgaggct cacgttgtcg ctgaagagga tgacgttgag gctcgccgag
      361 agcgccgcgc gcgcctcgac cgccgcgaag gcgcccggca ccgagatcag ggccaggttg
      421 gcctcgggca tccgccggat ggcgcccgcg gtggtgcgtg gccgcggccg ctcggcctgg
      481 ccgcgcgggg cgctccgggc cagcaggggc tccgccgccg cggcggcggc ctccgcgccg
      541 gttggcgtct cggcttccac cacgatcacc aggtcgttcg gcgtggcggt gcgggcctcc
      601 tcggtcagga ggccggcctg ggccagcacg tcatgattcg cgggggtgcc catcacgacc
      661 gccgcctcgc gcacgccggg ccgggcgcgg agcgcgcggg ccagggtgag cagcgtcacc
      721 gagtcgtggt aggcgccgcg cttcacgagg gtggcctggg ccatggatca ggcggccagc
      781 gcgtcccaag ccaggcgcat gccggcgagc gtgtccgctc ccgacgtcgc gcccagcccg
      841 cggagccgct cggccgcgag cgcggattcg aggctcgacc gcgcgcggag caccgccagg
      901 accggctcgg agaaacgccc cgcgagggcg tgaaggacga actcgcggcc gatcgcggtc
      961 gtgcgcgcgg ccgcggcggc agcgatggga gccaccgggc cgggctcgct cgcgagcgct
     1021 cccgcgtcga ccgcgtgcag caccgcgagt gccccgacca ggcaatcgtc gccggccggc
     1081 gtcagcccct cgccgagccc cagcagggcg cacgcccccg cgatcaggcg ggtggcatcg
     1141 cgacccgcga tgccggcggc caacgtcgcg cgcgcgccga cgccgaccgg cgagtcgaga
     1201 ccgggcgcgt ggcctgcgtg ggcgccgggc ggtgtcggga tggcgcgcgc ccggatcggg
     1261 cgcgcgcccg cggccgagca atcgacgcgg acggcccgcg cccacgacag ggtgagtccc
     1321 ggcgcgcgga gccgatgggg cccggcggtc acttccatgc ccggtcgcag tgcggacagg
     1381 agcgtgagat cgtcgacgac ggcggcgaag ggcgcgagca ggggcccggg cccgtggagg
     1441 gcgagcaggc tgccgtcgct ccactcgatg ttgaccgccc gcgcgaagac cgagtgcacg
     1501 cgcccctcga gctcggaggc ctcgagccgc cgggcgatct gggcgctcag gcggacgagg
     1561 tcgccggggc gccccgtcac cggcgcgcgg ctacttcggg gccgagatcc cgtaggcctt
     1621 gagcttgcgg tagaggtgac tgcgctcgat gcccagccgc tcggcggtgc gggtcatgtt
     1681 ccactcgttg gcccgcagct cgcccaggat gaaggcgcgc tcgaaggcct cgcgggcctc
     1741 cttgagcgtg cgctcgcgct gcgcgtcctc ggcggggggc tccgcgtcgc ggggacggag
     1801 cggcggcggc agatcctccg gcgcgatgac gtcgcgcggg ctcatgatca cgagccgctc
     1861 caccatgttg cgcagctcgc gcacgttgcc cggccagtcg taggagagga agtaggcgag
     1921 ggcctcgacc gagaccgtct tgggccgctt gccgttctcg gctgagaaca cggtcacgaa
     1981 gtgcgcgacg agggcgggca agtcctcttt ccgctggcgg agcggcggca cctcgatcgg
     2041 gatgacgttc aggcggtagt agagatcctc gcggaacccg cccgccgcga tgcgctcggg
     2101 caggttctgg ttggaggcgg cgaggacccg gacgtcgacc cggatggtgt ccttgccgcc
     2161 cacccgctcg aaggcctgct cctccagggc gcgcagcacc ttggcctggg tcttgaggct
     2221 catgtcgccg atctcgtcga ggaacagcgt gccctggtcg gccagctcga acttgccccg
     2281 ccgccgggcc accgctccgg tgaacgcgcc gcgctcgtgc ccgaagagct cggactcgat
     2341 cagctcctcc gggatcgcgg cgcagttgac ctcgacgaag ggcccatcgc gccgcgcgga
     2401 gagcgcgtgg atcgcgcggg ccaccagctc cttgcccgag ccgttctcgc cctggatgag
     2461 cacgcggccg ctcgagggcg ccgcgatcgc gatctgctgg cgcaggtgct cgatgagcgg
     2521 gctcttgccc acgatctcct ggccgcgctc gagctgggcc cgcaggttgc ggttctcccg
     2581 ctcgaggcgg gtgtggtcga gggcccggct ggcgatgagg agcgtcttct cgagcgagag
     2641 cggcttctcg atgaagtcgt aggccccgag cttggtggcc ttgaccgcgg tctcgatggt
     2701 gccgtggccc gagatcatga ccaccgcggt ctcgggccgc acccgcttca tctcggccaa
     2761 ggtgtcgagc ccgtcgatcc ccggcatcca gatgtcgagg aagatgatgt cgggcgtctc
     2821 ctcctggatc cgggcaatcg cctcctcgcc cgagcccacc gcggtgactc gatatccttc
     2881 gtcttcaaga acacccctta gagtcgtgcg gatcgcccgc tcgtcgtcca cgatcaggat
     2941 gtgctcgccc ggcatcgggt ccctcgccct caggcctgca ccggcgcgac caccggccgg
     3001 cccgcgggca gctcgatgac gaagcggctg ccgtgcggcg tgttgtcctc gacccggatg
     3061 gtgccgccgt ggtcggagac gatctggtgg acgatcggca ggccgagccc catgccggtg
     3121 gccttggtgg agaagtaggg cacgaagagg cgatcgcggt cgtccgccgc gatgccgggc
     3181 ccgtcgtcgg ccaccacgat gcgggcgcgg tgagcctcgg gcagccactc cgtctgcact
     3241 acaacctccc cggtgccgcc caccgcctcc accgcgttgt ccaccaggtt gaggaccgcg
     3301 cgcttgatct ggtccggatc cacctcgatg ggcggcagct cggagaagac ggagcggatc
     3361 gcgaggctcg gatgcgactc gcggtagagc acgaccacgc cttcgagcag ccgcgcgagg
     3421 tcggtgggct tcggcgtcag cgcgggcatg cgcgcgaagc gagagaactc gtcgaccagc
     3481 tgccgcagcc cgtccacctc ctggatgatg gtgccggtgg cctcctcgag cagccgcttc
     3541 tcctccgggc tccggttgga ggcgaggcgg cgccgcaggc gctcggccga gagctggatg
     3601 ggggtgagcg ggttcttgat ctcgtgcgcg atgcgctggg cgacctcgcg ccaggcggcc
     3661 aggcgctggg ccttgaggag ctcggtgagg tcgtcgaagg ccagcaccat gcccacgtag
     3721 ccgccgtcgg gcccgcgcag ggcggtggcg gaggcgagca gggccaccgc ctggccgtgg
     3781 cgccgcaggt gcacctcgcg gtcgagcatg ccctcgcgca cccggcccat gcgctggatc
     3841 agcgcgtcga tctccacgta ctcgggggag cggaacaccc gggtggcgga gcggccctgg
     3901 accgtcaccg cgtcgagccc gagcagccgc tcggcggccc cgttgatggt ggtcacgcga
     3961 ccgtccgggt cgagggagac cactccggtc gcgaccgcct ccagcaccgt ctcggtgtag
     4021 cggcggcgct cctccatctc cccgtgcttg gcctggagat cgcgataggt ctcctcgagc
     4081 ttggacttgg aggcggccag gtccccggtc atccggttga acgagtcgac caggatgccg
     4141 atctcgtcgt cggcccgcac ctccaccttg tagttgaggt tgcccgccgc gacctctctc
     4201 gttccttcgg ccagcatctg gatcggctcg gtgatccccc gggccaggta gaggccgaac
     4261 cacgtggccg agaacacgat gatcagggtc atgaggagga agagcaggat gtagatgccc
     4321 ttgatcgggt tcttgagcag gcggagctgc ttgtactcct ggaaggcctg gctgatgttg
     4381 cgcaggcgcg cctcgagccg ctgcggcacg tggatggcca ccaccatcgc cccgctcacc
     4441 gcctggtcgg cgtcgcgtat cggcaccatc gcctggatca tgtcgccgtt gtccagctcg
     4501 tggacggtgg tgatctcctg gccgccgagg gcctgcttca cgtgctcgct gttggtcgac
     4561 cgggtcggga ccttggccag caccgggttc ttgacgtgga ccagctcctg cccgtcgcgg
     4621 ttgaagatgg tgatcgcggc caccccgagc cgctcctgct gctcggtcag gtagccggcg
     4681 agggcctcgc gcttgctctc ggcgaggagc ccgtcgcgct cgaccacctt ggccatgtag
     4741 cgcccgtggc ggagcgcggt ggactcgagg ctctggtagt aggtctgggc cacctccatc
     4801 gcctgatcga gcggacgctc gacctggggc ttgaaccagc cttcgatgga ggtggtgatg
     4861 aagttcgacg cgatcaggaa gatcaggatg ccggggacga gggagagggc caggaaggtg
     4921 aggaccagct tggccttgaa gcgggagccg atgatcttgt ggcggcgctc gaaggagagc
     4981 ttgacgaggt tgcggaacag cagcaccagc agcagcagga agacgatcag gttcaggttg
     5041 aacaggaaga agacgatgag gttcgaggcg aacgggatct tgggcgcgcg gatgcccacc
     5101 tcgaacacgg tggccaccac cagcaggatc aggatgccgc tgatgatgag gaggttgcgc
     5161 ttccgcttct cctgctcgtc gggccgctcg ggacgacggg tgagcggcgt cactggttcc
     5221 gcaccggggt gagcacgctc gactgcaccc acggcgtctc ctcggcctcg ccggtcatgc
     5281 gggcgaacca ggagttgacg ccgccgaggg acacgtcgga gcgcacccgc acgtagtaga
     5341 gctcctgctt gtcgagcgcg gccaccgggg ccagctgtcc cacccgcagc tcggagatca
     5401 cccgctgggc ctcgcgcagg tccttggtga ggaaaggctc gcgggtctcg cccttgaggg
     5461 aggcgacctt gtattccttg gtcagcacgt tgtaggtgag ctggcgctcc accatgcgcg
     5521 cctggagccg ccggtccacg cccatgcggg tgtactgcca cagctcgacg tagtagcgca
     5581 cgtgggccgc gatgccggtg tggatggact catggagcga gtcgggcacc gccccgaaca
     5641 ggacgatggt gaccgtgacg tcgtagtcgt tgaggaagac cgacaggttg ctgatcctca
     5701 cctcggcacc gaccggcgcg ggccccgacg gcagacccac caggagggcg acgagcaggc
     5761 tcgcgcccgg caggatgcgg cggagcggac gaccgcgagt gcccatggcc aagacggacc
     5821 attatagcgt cgggtccggc gggggcaccg gttcgagccg gccgggggcc gggtgggggc
     5881 gggaagccgg ggaagggcgg cgcgacgcgg gcggccggcc tagagggcgg ccacacccgg
     5941 gaggaagccg tcaccggtgc tggccaccgg cacgggagcg gccggccgga tgacccgggc
     6001 gaccgagcga cgccggtcgg cggcgaccgc cttctggatc gcgctcacca gctgatagtt
     6061 cagggcgtgg ccggcattgc gcccgaccac cagggcccgg agcgggcggc ccaggaggaa
     6121 caggtccccg accaggtcga ggatcttgtg gcgaacgaac tcgtcgggat agcgcaggct
     6181 gtcgttcagc acggagcgct tgcccaccac caccgcgttc tccagcgagc cgccgcgggc
     6241 gagaccgttc tgccgcatca cgggcacgtc gcggaggaag ccgtaggtgc gggcgggggc
     6301 cagctcctgg tcgaacacgt cctcggtgat cccgtaggtg cccgcctgca ggccgatgat
     6361 cgggtggctg ttgtcgagcg tgtagctcac ccgcagggac tcggcgggca gcacctcgag
     6421 ccagcggctc tcggttccca cgcggatcgg ctcgctcagc acgatgggtc ggcgcggcgc
     6481 gggcaggctc gcgcgtccgc ccgagcgcag cagatccatg aacggcttgg cgctgccgtc
     6541 ggccgcgggt acttccggcc cgtggatgtc gacgaccgcg ttgtcgatgc cgagcgccgc
     6601 cgccgcggcc atgaggtgct cgatggtccg cacccgcacg ccgaaggctc cgaccgtggt
     6661 cgcggagttc gtgtccacca cgtggtcggc attggccggg atcagcgtgc cgtcggcggc
     6721 gcggaagagg atgcccgtgt cggcgcccgc cggagagacg gtcatctcga ccggctcacc
     6781 cgagtgcagc ccgattcccg cgagcttcac cggctgcctc agagtctgct ggagatccat
     6841 ggccgcggct atagtaggca agccagatgc catgggccaa ctatctaact aagtatatgt
     6901 tttaactgat gtttacactt atatcgcaag gcggtctcta tgtaattggt tgcaagaaag
     6961 acacaccccc gggcccccgc atgtgtcagt ttcgcaacac tagatctcga ggctcgccct
     7021 cagggagaag cccttcgatc cggactccac ggagaggccg tcccgggaga cattgcggat
     7081 ggcggccaga acccggtgtc gggagggatc gatctcctcg ccacgcagga tggcgtgcag
     7141 ccggagcgct tcgccgcccg ccgacggggc ggtctcgaac actgcgaact cgagcgagcg
     7201 gcaggcgaag ccctccacct cgtgcacgta gaggcactcc tggagccagg cgtggagcag
     7261 ggcgggcagc ccgacaccgt gtgccctgac ttcgcgcgtg tcgccgtcgc cgacggtgtc
     7321 gggatccacc gccgccgcga agagaccgag ggcgatgcgc gtgaacgcct ccgacagggt
     7381 ggcgcccgtc gcggtcagct ccacgtcagg cgcggcccag gtcgccgtac cgcgactgca
     7441 ggagcgccac cgccaccgcg cccgcggtct cggtccggag cacgcgcggg cccaggccgg
     7501 ccaccagcgc cccggccgcg gcgaggcccc gcacctcctc cgcggtgagc ccgccctccg
     7561 gtcccacgat cagcgtcgca gccgggcacg gcccggcggg aagccgccct tcgagcccgc
     7621 cgggctcttc ctcccagaag cagatcagga ggccgtcacg ctccggatcg ggccgccagg
     7681 tcgagagcgt gcgcggcgcc gtgatctcgg gcaccgcggc ccggcc
//
LOCUS       DATMCV010000969         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_392444, whole genome shotgun sequence.
ACCESSION   DATMCV010000969 DATMCV010000000
VERSION     DATMCV010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392444"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VNU03_22135"
     CDS             <1..767
                     /locus_tag="VNU03_22135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP77239.1"
                     /translation="AIAARTTRLKFGPSVVVLPYRTPVVAAKEMATVDWLSQGRLFPA
                     VGVGVELPREFDASGVPFAERGRRTDEAIRVMRLLWTQDEVTFQGDFYKLDRVSIFPK
                     PWQTPPPIWIGGKSLAAMRRTARLGEGWIPSFITPDEMRTGIQKVHDLAAAAGRQVPE
                     DHFGTLINYALADSEAAALAMAQPYIPRGRVDESTMRQCTAFGPAGRLLEKVEEYVKA
                     GASKFILRPLCPPDRMLEQLALVAEHVCPEYHHRSD"
     gene            800..1777
                     /gene="sppA"
                     /locus_tag="VNU03_22140"
     CDS             800..1777
                     /gene="sppA"
                     /locus_tag="VNU03_22140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWP77240.1"
                     /translation="MHRLRPLALAILVALAPPFLGGCSLVTLDFQPKIRALEEETVEG
                     KGSSKILMIDLSGMLSEDLPSFSLGAPPPHVPLLARVREELARAEKDDQIRGLIVRIN
                     SPGGTITASDVLYHEILTFKQRKKVPVVAAIMDVGASGGYYAALAADTIVVNPTTITG
                     SIGVVMVTLNAQGLMQKIGVAPLAIKSGPMKDAGSPFRSLTAPELAVFQTMIDEFYGR
                     FVGLIVQSRKLPEDRVRALADGRVYTADQALRLGLVDRIGYLEDVVKLTREAAKLDEA
                     RVVMYHRPKEYRSNIYSSSQAPATAESTLGQFATVLGVGGPRFLYLWWP"
     gene            complement(1774..>2835)
                     /locus_tag="VNU03_22145"
     CDS             complement(1774..>2835)
                     /locus_tag="VNU03_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77241.1"
                     /translation="IRPEEPIHRDATLVRTTADRAAALTHQLLAFSRKQVLQPQVLNL
                     NAVVTGMEPMLGRLIGEDIDLSVIPAEGLGRVRADPGQLEQVIVNLVVNARDAMPEGG
                     RLTIETADVELDAAYASRHLTVPAGSYVMLEVTDTGEGMDEQTRSRVFEPFFTTKGPG
                     KGTGLGLATVYGIVKQSGGDIQLSSERGRGTSFKIYLPRVAEQASEPGPTRSAAAPVP
                     RGDETVLLVEDEPEVRDLAREILEASGYTVLQACDPQDAILIAERHAGPIRLLLTDVI
                     MPRQSGRALAERLRPLRPELQVLYMSGYTNEAIVRHGVLEPDTMFIQKPFTPAALGHK
                     VRDALDRDRPRPASAEPPA"
BASE COUNT          405 a          996 c          990 g          444 t
ORIGIN      
        1 ccgcgatcgc cgcgcgcacc actcggctca agttcgggcc gagcgtggtg gtgctcccgt
       61 atcgcacccc ggtggtggcg gccaaggaga tggccacggt ggactggctc tcgcaggggc
      121 ggctcttccc cgcggtgggg gtgggagtcg agctgccgcg cgagttcgac gcctccggcg
      181 tgcccttcgc ggagcgcggg cggcgcaccg acgaggccat ccgggtgatg cgcctgctct
      241 ggacgcagga cgaggtgacc ttccagggcg acttctacaa gctggaccgg gtgtcgatct
      301 tccccaagcc atggcaaacg cctccgccga tctggatcgg gggcaagagc ctagcggcca
      361 tgcgtcggac cgcgcggctc ggcgagggct ggatcccgtc cttcatcacg ccggacgaga
      421 tgcggaccgg gatccagaag gtgcacgacc tggccgccgc agcggggcgc caggtgccgg
      481 aggaccactt cggcaccctg atcaactacg cgctcgccga ctcggaagcg gccgcgctgg
      541 cgatggccca gccctacatc ccgcgcgggc gggtggacga gtccaccatg cgtcagtgca
      601 ccgccttcgg tccggccggg cgcctgctcg agaaggtgga ggagtacgtg aaggcgggcg
      661 cctcgaagtt catcctgcgt cccctctgcc cgcccgatcg gatgctcgag cagctcgccc
      721 tcgtggccga gcacgtctgc ccggagtatc accaccgctc cgattaggcc cgtctcgcgg
      781 ggccccgtat actgcaggca tgcatcgact ccgacctctg gctctcgcga tcctcgtcgc
      841 cctcgccccg ccgttcctgg gcggctgctc gctggtcacc ctcgacttcc agcccaagat
      901 ccgggcgctc gaggaagaga cggtggaggg caaggggtcg agcaagatct tgatgatcga
      961 tctctcgggg atgctctccg aggacctccc gagcttctcc ctgggcgcgc cgcccccgca
     1021 cgtcccgctc ctggcccgcg tgcgggagga actggccagg gcggagaagg acgatcagat
     1081 acgcggcctg atcgtgcgca tcaacagccc gggcggcacc atcaccgcct ccgacgtgct
     1141 ctaccacgag atcctcacct tcaagcagcg gaagaaggtc ccggtcgtcg ccgcgatcat
     1201 ggacgtgggc gcctccgggg gctactacgc ggccctcgcc gccgacacca tcgtggtcaa
     1261 ccccaccacc atcaccggca gcatcggggt ggtgatggtc accctcaacg cccaggggct
     1321 gatgcagaag atcggggtcg cgccgctcgc gatcaagtcg gggccgatga aggacgcggg
     1381 ctcgcccttc cggtctctca ccgcgcccga gctggccgtg ttccagacca tgatcgacga
     1441 gttctacggg cggttcgtgg ggctcatcgt gcagtcgcgc aagctgcccg aggatcgggt
     1501 gcgcgcgctc gccgacggcc gcgtctatac cgcggaccag gcgctgcgcc tcggcctggt
     1561 cgaccgcatc ggctacctcg aggacgtggt caagctgacc cgggaggccg ccaagctcga
     1621 cgaggcgcga gtggtgatgt atcaccgccc caaggagtat cgctccaaca tctactcgag
     1681 cagccaggcc ccggccacgg cggagagcac cctgggacag ttcgcgaccg tgctcggggt
     1741 cggcgggccg cgcttcctct atctttggtg gccctaggcg ggcggctcgg ctgatgcggg
     1801 ccggggccgg tcccggtcga gcgcgtcgcg gaccttgtgt ccgagcgcag cgggcgtgaa
     1861 gggcttctgg atgaacatgg tgtccggctc gaggaccccg tggcgcacga tcgcctcgtt
     1921 cgtgtagccg gacatgtaga gcacctgcag ctcgggccgg agcggacgta gccgctcggc
     1981 gagggcccgc ccgctctgcc gcggcatgat gacgtcggtc agcagcagcc ggatcggccc
     2041 cgcgtggcgc tccgcgatca ggatcgcgtc ctgaggatcg caggcctgga gcacggtata
     2101 gccgctcgcc tccaggatct cgcgcgcgag gtcccgcacc tccggctcgt cctccaccag
     2161 cagcaccgtc tcgtcaccgc gcggcaccgg ggcggccgcc gacctcgtcg ggccgggctc
     2221 gctcgcctgc tcggccacgc gcgggaggta gatcttgaag ctggtgccgc gtccccgctc
     2281 gctggagagc tggatgtcac cgccgctctg cttgacgatg ccgtacacgg tggccagccc
     2341 gaggccggtg cccttcccgg ggcccttggt ggtgaagaag ggctcgaaca cgcgcgagcg
     2401 tgtctgctcg tccataccct caccggtgtc ggtcacctcc agcatcacgt aggacccggc
     2461 cggcaccgtg agatgccggc tcgcgtaggc cgcgtcgagc tccacgtcgg cggtctcgat
     2521 cgtgaggcgt cccccctcgg gcatcgcgtc gcgggcattg acgaccaggt tgacgatgac
     2581 ctgctcgagc tggccggggt cggccctgac gcgtccgagt ccctcggcgg gaatcaccga
     2641 gaggtcgatg tcctctccga tgagccggcc cagcatcggc tccatgccgg tcaccaccgc
     2701 gttgaggttc agcacctggg gctggagcac ctgcttgcgg ctgaaagcca ggagctggtg
     2761 ggtgagcgcc gccgcgcggt ccgcggtggt gcgaaccagg gtggcgtcgc ggtgaatcgg
     2821 ctcctcggga cggat
//
LOCUS       DATMCV010000970         2374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_924269, whole genome shotgun sequence.
ACCESSION   DATMCV010000970 DATMCV010000000
VERSION     DATMCV010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924269"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VNU03_22150"
     CDS             <1..973
                     /locus_tag="VNU03_22150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP77242.1"
                     /translation="EQVAKYLDAPYNQRPHTFPFYPWDGWDRRMLGTLGDNAGTAEEW
                     LRALDLGGMEQTVLYPTLGLFLSFLKDREWAVALCRAYNTFLHEEFVKKSPRLHAVAL
                     LPVQDAEAAARELRRAVTELGLVGAMLAADGTHLLGDARFLPIYEEADRLDVMLGIHA
                     SGSHLGGGGLELFPRFIQAHTCSHAFGQMRQITSMIFEGIPERFPRLRIAYLEAGCGW
                     APYWTERMDDEYAKRAAEAPALKRKPSDYVRSGSIFFSCEADEWLLPQALKLIGENQI
                     VYASDFPHWDHSYPASLDEIRDRGDITDVQKRKLFADNARRLYGLKG"
     gene            1041..1883
                     /locus_tag="VNU03_22155"
     CDS             1041..1883
                     /locus_tag="VNU03_22155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77243.1"
                     /translation="MRGVDLVIRAGEVVAMLGPNGAGKTTSISLMLGLRQPTAGQVRL
                     FGLPPTDRRARSRCGVMLQESGTTSVLTVREIVDVFRVYYPAPLPAERAIAMAGLTEQ
                     AAVRIGTLSGGQRQRLYFALAICGDPDILFLDEPTVGMDVEARRAFVTSIQTLAAAGK
                     TIVFTSHYLREAEELARRIIVIDRGTVIADASPQELKARVPGKKITLVAGRPFTDKDL
                     AGLPASVISTSGERASLLSNEPAAVLRELFRRGVDVVDLEVTGADLEEAFLALTRRAE
                     AATP"
     gene            1880..>2374
                     /locus_tag="VNU03_22160"
     CDS             1880..>2374
                     /locus_tag="VNU03_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77244.1"
                     /translation="MSTGPRPEVAPLGRMLLVQARSEMRMRWRIPAFSVTIVALPVLF
                     FTFFGLPYVKQTGPNGINLGAYLIASFAAYSVGSVMVFGFGIGVATERGLKVDVLMRA
                     TPLPPLIAILAKVLNALAYALLSLIVLIVYGVIVGGIRQDLLVWLDMIARLLAGSVPF
                     IALGF"
BASE COUNT          328 a          856 c          827 g          363 t
ORIGIN      
        1 cgagcaggtc gcgaagtatc tcgacgcgcc ctacaaccag cgtccgcaca cgttcccttt
       61 ctatccctgg gacggctggg accggcgcat gctcggcacg ctgggcgaca acgcggggac
      121 cgcggaggag tggctgcgcg cgctcgacct gggtggcatg gagcagaccg tgctctatcc
      181 gaccctcggg ctctttctct ccttcctgaa ggaccgcgag tgggcggtgg ccctctgccg
      241 cgcctacaac accttcctcc acgaggagtt cgtcaagaag agcccgcggc tgcacgcggt
      301 cgccctgctg ccggtgcagg atgccgaggc ggccgcgcgc gagctgcggc gcgcggtgac
      361 cgagctgggt ctcgtcggcg cgatgctcgc ggccgacggc actcacctgc tgggcgacgc
      421 gcgcttcctg cccatctacg aggaggccga ccggctcgac gtcatgctcg gcatccacgc
      481 atccggctcg cacctgggcg gtggcgggct cgagctcttc ccgcgcttca tccaggccca
      541 cacctgctcg cacgccttcg ggcagatgcg ccagattacc tcgatgatct tcgagggcat
      601 cccggagcgc ttcccgcgcc tgcgcatcgc atacctcgag gcaggctgcg gctgggcccc
      661 ctactggacg gagcgcatgg acgacgagta cgccaagcgc gcggccgagg ccccggccct
      721 caagcgcaag ccgagcgact acgtgcggag cgggtcgatc ttcttctcgt gcgaggccga
      781 cgagtggctg ctgccacagg ccctcaagct gatcggggag aaccagatcg tctacgcctc
      841 cgacttcccg cactgggacc acagctatcc cgcctcgctc gacgagatcc gcgaccgcgg
      901 cgacatcacc gacgtgcaga agcgcaagct gttcgccgac aacgcgcgga ggctctacgg
      961 cctcaaggga tgacgcgggg gcatccgccc cggcggtagc cctcgtcggc gcgcgcaagc
     1021 gctacgggtc tgtcgaggcg ctgcgcggcg tcgacctcgt catccgcgcc ggcgaggtgg
     1081 tggccatgct cggccccaac ggggcgggca agaccacctc gatcagcctc atgctcgggc
     1141 tccgccagcc caccgcgggc caggtgcgcc tcttcgggct gccgcccacc gaccgccgcg
     1201 cgcggagccg ctgcggggtc atgctgcagg agtcgggcac cacctcggtg ctcacggtgc
     1261 gcgagatcgt cgacgtcttc cgcgtctact atcccgcccc cctcccggcc gagcgcgcca
     1321 tcgcgatggc cgggctcacc gagcaggccg cggtgcggat cggcacgctc tcgggcggcc
     1381 agcgccagcg gctctacttc gcgctcgcga tctgcggcga ccccgacatc ctcttcctcg
     1441 acgagcccac cgtgggcatg gacgtggagg cgcgccgggc gttcgtgacc agcatccaga
     1501 ccctggccgc cgcgggcaag accatcgtgt tcaccagcca ctacctgcgc gaggccgagg
     1561 agctggcgcg gcggatcatc gtgatcgacc gcggcaccgt gatcgcggac gcgagcccgc
     1621 aggagctgaa ggcgcgcgtg ccgggcaaga agatcaccct ggtcgcgggg cggccgttca
     1681 ccgacaagga tctggcgggc ctgccagcca gcgtgatcag cacttccggg gagcgggcga
     1741 gcctgctcag caacgagccg gccgcggtgc tgcgcgagct cttccgccgc ggggtggacg
     1801 tggtggacct ggaggtgacc ggcgcggacc tggaggaggc gttcctcgcg ctcacccggc
     1861 gcgcggaggc ggccaccccg tgagcacggg cccgagaccg gaggtcgcgc cgctcgggcg
     1921 catgctgctg gtgcaggcgc ggagcgagat gcggatgcgc tggcgcatcc ccgcgttcag
     1981 cgtgaccatc gtggccctgc cggtgctgtt cttcaccttc ttcgggctgc cctacgtcaa
     2041 gcagaccggc ccgaacggca tcaacctggg cgcgtacctg atcgcctcgt tcgcggccta
     2101 cagcgtgggg agcgtgatgg tcttcggctt cggcatcggg gtcgccaccg agcgcgggct
     2161 caaggtggac gtgctgatgc gcgcgacgcc cctgccgccg ctgatcgcga tcctcgccaa
     2221 ggtgctgaac gcgctcgcct acgcgctgct gtcgctgatc gtgctgatcg tctacggcgt
     2281 gatcgtgggc gggatccgcc aggatctgct cgtgtggctc gacatgatcg cgcggctgct
     2341 ggccggctcg gtgccgttca tcgcgctggg cttc
//
LOCUS       DATMCV010000971         3074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_519305, whole genome shotgun sequence.
ACCESSION   DATMCV010000971 DATMCV010000000
VERSION     DATMCV010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519305"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VNU03_22165"
     CDS             complement(<1..1097)
                     /locus_tag="VNU03_22165"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP77245.1"
                     /translation="MKIFDEHHDMFRQAVRAFVQKEVEPHVEEWEEAGQIPKSIWPRM
                     GELGFLGVEYDEKYGGAGADVLTTAVLCEEMARARCASLAMAVGVHTDMASPHLYWTG
                     SEALKERYLPAICRGEALTAIAVTEPGGGSDVAAIRTRAVRAGDHYVLNGSKMFITNG
                     VMADLYFVAARIQEERPLTTGPLTPGPLTLPSPQGGEGGKRHRGISMFLVERSTPGFS
                     VSRKLDKMGNRASDTAELAFQDMRVPAENLLGQEGGGFYEVMRVFQRERLVAGLHAVA
                     GCERALEDTIAYVQQRQAFDGPLSGKQVIRHRIADLATLIEAGRWLTYAACVKFRDRE
                     DAVKEISMVKLFTAEMAQKVAYECVQLHGGYG"
     gene            complement(1118..2647)
                     /locus_tag="VNU03_22170"
     CDS             complement(1118..2647)
                     /locus_tag="VNU03_22170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP77246.1"
                     /translation="MAELSVHAFTVYDVITRGAAVHRDAPAVIQGDRTLSFGDFHRRV
                     DELAAGLAGLGIGRGERICVLAQNDAAYLDLYGACARQGIIAYPINWRLTGPEVERVV
                     ERARPAMMVVDATTLPLVGEWIETKGVGHRYQLGEGAGPGFTPFAALYRPGTAPPPAA
                     VGPEDAFAVISTAAVDVIPRGAVLTHANVLAANLTAIGALGLTARDRYLAVLPLFHVT
                     ALGMSLAHLHAGGAVVVVPRFDAEEAVRLIDRHQVAHVSDFPPVLASLLDAAEKVGSA
                     LPSLTHVSGLDSPATIGRLHEKTRARFWTGFGQSETSGFVTIQPVTDKPGASGRPVPA
                     AQVGIVDEYDRQVPTGTPGEIVVRGPLVFQGYFGQPDVTAHTFRNGWHHTGDVGRFEA
                     DGYLHYVKRKPEKELIKPGGENVYPAEVETVIVQMPAVTGVCVYGVPDPRWGEAVKAV
                     VEIKGGSRLTAQQVGDFVGERIARFKRPHVVVFTDALPRGADGAVDRDAVKARWGDGR
                     E"
     gene            complement(2635..>3074)
                     /locus_tag="VNU03_22175"
     CDS             complement(2635..>3074)
                     /locus_tag="VNU03_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP77247.1"
                     /translation="IERVYEYFPRLRERRSVQAGYLSGGEQQMLAIGRALMSGPRVIL
                     LDEPSLGLAPMLVEEIFTIIQRLNREQKLTVLLVEQNATLALGIAEHGYVMENGRIVL
                     EGSAEALRENADIKEFYLGLTEVGGRKSYRDVKHYKRRKRWLS"
BASE COUNT          424 a         1130 c         1058 g          462 t
ORIGIN      
        1 ccgtagccgc cgtggagctg cacgcactcg taggccacct tctgggccat ctcggcggtg
       61 aacagcttca ccatcgagat ctccttcacc gcgtcctccc ggtcccggaa cttgacgcag
      121 gccgcgtagg tcagccagcg cccggcctcg atcagagtgg ccaggtccgc gatccggtgc
      181 cggatcacct gcttgccgga gagcgggccg tcgaaggcct ggcgctgctg gacgtaggcg
      241 atggtgtcct cgagggcgcg ctcgcagccg gccaccgcgt gcagccccgc caccaggcgc
      301 tcgcgctgga agacgcgcat gacctcgtag aagccgccgc cttcttgccc gagcaggttc
      361 tcggccggca cccgcatgtc ctggaaggcc agctccgcgg tgtccgaggc gcggttgccc
      421 atcttgtcga gcttgcggct caccgagaac cccggcgtgc tccgctccac caggaacatc
      481 gagatccccc ggtgccgctt cccaccctct cccccctggg gagagggcaa ggtgaggggc
      541 ccgggggtga ggggcccggt ggtgaggggt ctttcctcct ggatccgcgc cgccacgaaa
      601 tagagatccg ccatcacccc gttggtgatg aacatcttcg agccgttcag cacgtagtgg
      661 tccccggcgc gcaccgcgcg cgtgcggatc gcggccacgt ccgacccgcc gccgggctcg
      721 gtgaccgcga tggcggtcag cgcctccccg cgacagatcg cgggcaggta gcgctccttg
      781 agggcctcgc tcccggtcca gtagaggtgg ggcgacgcca tgtcggtgtg cacgccgacc
      841 gccatcgcca gggaggcgca tcgcgcccgg gccatctcct cgcagagcac cgcggtggtg
      901 aggacgtcgg cgccggcgcc gccgtacttc tcgtcgtact cgacgccgag gaagcccagc
      961 tcgcccatgc ggggccagat cgacttgggg atctggcccg cctcctccca ctcctcgacg
     1021 tgcggctcga cctccttctg gacgaaggcg cgcaccgcct ggcggaacat gtcgtggtgc
     1081 tcgtcgaaga tcttcatgcg ctcaagtcta ctctggacta ctccctgccg tcaccccacc
     1141 gggccttcac cgcgtcccgg tcgaccgcgc cgtcggctcc gcgcggcagg gcgtcggtga
     1201 acaccaccac gtgcggacgc ttgaagcggg cgatccgctc gcccacgaag tcgcccacct
     1261 gctgggcggt gagccgggac ccgcccttga tctccaccac cgccttcacc gcctcgcccc
     1321 agcgtgggtc cgggacgccg tagacgcaga ccccggtgac cgcgggcatc tgcacgatga
     1381 cagtctcgac ctccgccggg tagacgttct cgccgcccgg cttgatcagc tccttctcgg
     1441 gcttgcgctt gacgtagtgc aggtagccgt cggcctcgaa gcggccgacg tcgccggtgt
     1501 ggtgccagcc gttgcggaag gtgtgggcgg tcacgtcggg ctggccgaag tagccctgga
     1561 agacgagtgg gccccgcacc acgatctccc ccggggtgcc ggtcgggact tgacgatcgt
     1621 actcgtccac gatgcccacc tgcgccgcgg gcaccggtcg gccggaggcc cccggcttgt
     1681 cggtcaccgg ctggatggtc acgaagccgc tcgtctccga ctggccgaac ccggtccaga
     1741 agcgcgcccg cgtcttctcg tggaggcgcc cgatggtcgc gggcgaatcg aggccggaga
     1801 cgtgggtcag gctcggcagc gcgctcccga ccttctcggc cgcgtcgagc aggctcgcca
     1861 gcaccggtgg gaagtcggag acgtgcgcca cctggtgccg gtcgatcagg cgcaccgcct
     1921 cctccgcgtc gaagcgcggc accaccacca ccgccccgcc cgcgtgcagg tgggccagcg
     1981 acatgccgag cgcggtcacg tggaagagcg gcagcaccgc caggtaacgg tcgcgcgcgg
     2041 tgagaccgag ggcgccgatc gcggtgaggt tggccgcgag gacgttggcg tgggtcagca
     2101 ccgcgccccg cgggatcacg tccaccgcgg cggtggagat gaccgcgaag gcgtcctcgg
     2161 gcccgaccgc ggccggcggc ggcgcggtcc cggggcgata gagggcggcg aacggggtga
     2221 agccgggccc cgccccttcc ccgagctggt agcggtgccc tacccccttg gtctcgatcc
     2281 actcgcccac cagcggcagg gtcgtcgcgt ccaccaccat catggctggc cgggcgcgct
     2341 ccaccacccg ctccacctcg gggccggtga gccgccagtt gatcggatag gcgatgatcc
     2401 cctgccgcgc gcaggccccg tagaggtcca ggtaggccgc gtcgttctgg gccagcacgc
     2461 agatgcgctc gccccgtccg atgccgaggc cggcgaggcc ggcggccagc tcgtccaccc
     2521 ggcggtggaa gtcgccgaac gagagcgtgc gatcgccctg gatgaccgcg ggcgcgtcgc
     2581 ggtggacggc ggccccccgc gtgatcacgt cgtagaccgt gaaggcgtgg acgctcagct
     2641 cagccaccgc tttctccgct tgtagtgctt cacgtcgcgg taggacttgc ggccgcccac
     2701 ctcggtgagc cccaggtaga actccttgat gtccgcgttc tcgcgcaggg cctccgcgct
     2761 cccctcgagc acgatgcggc cgttctccat cacgtagccg tgctcggcga tgccgagggc
     2821 caacgtcgcg ttctgctcga ccagcagcac ggtcagcttc tgctcgcggt tcaggcgctg
     2881 gatgatggtg aagatctcct ccaccagcat gggggccagg ccgagcgagg gctcgtcgag
     2941 gaggatcacg cgcggccccg acatgagggc ccggccgatc gcgagcatct gctgctcgcc
     3001 gcccgagagg tagccggctt gcaccgaccg gcgctcccgc agccggggga agtactcgta
     3061 cacccgctcg atgc
//
LOCUS       DATMCV010000972         3799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_253773, whole genome shotgun sequence.
ACCESSION   DATMCV010000972 DATMCV010000000
VERSION     DATMCV010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253773"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1422)
                     /locus_tag="VNU03_22180"
     CDS             complement(49..1422)
                     /locus_tag="VNU03_22180"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWP77248.1"
                     /translation="MAELNPYIFRAYDVRGKVGSDITPPVFEQVGRAYGTLVKRRGGR
                     TVALGMDNRVSSAALKAAFGAGVLATGLDVVDIGVNHTPLLYFATAHWGLDGGATVTG
                     SHNPVSDNGVKMVHAAAAPLTEEEIQGLLATIRSGDFEQGRGSQTARDPREEYLGAIT
                     GHVRLARRLKVVVDAGNGIAGVFAPELLRRLGCEVVELYCESDGNFPNHLPDPEMEEN
                     MRDLVAKVTEVRADVGLAYDGDADRVGIVDEKGRRHEADLLLALLARDLLVRHPGAKI
                     IFDVKSSQVLVDDIRRHGGEPIMWKTGHSLLKRKMREDGILLGGEISGHMFFGENWYG
                     VDDGILASCRFLQLVAAGLRPASAHFDTLPHLHSTPELKAPCPDDRKFQLVAELAREF
                     KSRYETIDIDGARIIFPEGWGLVRASNTNPYLTMRFEGRTAAAVEQMMQIVYDALRRY
                     PYVTLPK"
     gene            complement(1436..1828)
                     /locus_tag="VNU03_22185"
     CDS             complement(1436..1828)
                     /locus_tag="VNU03_22185"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP77249.1"
                     /translation="MVKIERYAAKGVWDPPTYSQAVKVTGAQTILYLAGQVAYDDKGG
                     PAHRGDFKAQARAVFQAIKAQVEAGGGTMDNIVKINTYLTDIRNRADLGTIREEFLGK
                     KTCASTLVGVAALAMPEWLIEIEAIAIL"
     gene            1899..2285
                     /locus_tag="VNU03_22190"
     CDS             1899..2285
                     /locus_tag="VNU03_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWP77250.1"
                     /translation="MDDALKETTRKTARMLFHSIKSGVGFDTWKKFDKDLARELSMFF
                     TGKLYSREVISQKQRELCAVASLTVLNRRNEVHAHIHAALNVGASRQEVTEVIFQQVT
                     YGGMPVVVEALQVFKEVLVERGEWQD"
     gene            2273..3049
                     /locus_tag="VNU03_22195"
     CDS             2273..3049
                     /locus_tag="VNU03_22195"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWP77251.1"
                     /translation="MAGLTAAVPDVVTAMIRAAEAAGEVAMRYYRGGFEVTIKPDQTP
                     VTQADREAEQAIKAILRRACPDHGFLGEEFGEEGPKERRFIIDPIDGTKNFIRRVPIW
                     AVLIGLEEAGQVTAGVVLNPASGEMLWAHRGDGAWDRDGRLRVSDCGRLAEATVLHSD
                     LHLLRQGPHWDAFLRMVDGSRRSRGFGDYYGYGLVAAGKAELYLEVDLKPWDVAPMLV
                     LLEEAGGRLTDFSGRATIYDGSVLATNGRLHEQILALLRR"
     gene            3060..>3799
                     /locus_tag="VNU03_22200"
     CDS             3060..>3799
                     /locus_tag="VNU03_22200"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HWP77252.1"
                     /translation="MRLEPLRLIRPAPALAAAIASPPYDVVSRAEAAALARDNPHSFL
                     HIGRAEIDLPDEVDPYDPLVYVRARENLAALLGPGALRREPGPSLFLYRQVMEGRTQV
                     GVVGGVRIEDYETGLIREHETTRPDKEADRTRHLLALDAQAEPVLLAYRGRPEIDRLV
                     AETLAGPPLDDFTTGDGVAQAVWRVADAAPYLTAFASVQAAYVADGHHRSAAAARAAE
                     KRRAESSRPRGDRAYEWLLSVLVPAAQLR"
BASE COUNT          544 a         1325 c         1361 g          569 t
ORIGIN      
        1 ccctcaccct gccctctccc cggaggggag agggttactg agggggggtt acttcggaag
       61 ggtcacgtag gggtagcggc ggagggcgtc gtagacgatc tgcatcatct gctcgaccgc
      121 ggccgcggtg cgtccttcga accgcatggt gaggtagggg ttcgtgttgg aggcgcgcac
      181 gaggccccag ccctccggga agatgatgcg cgcgccgtcg atgtcgatgg tctcgtagcg
      241 gctcttgaac tcgcgcgcca gctccgccac gagctggaac ttccggtcgt cggggcaggg
      301 ggccttcagc tccggcgtcg agtgcaggtg cgggagcgtg tcgaagtgcg cggaggcggg
      361 gcggagcccg gcggccacca gctggaggaa ccggcaggag gcgaggatgc cgtcgtccac
      421 cccgtaccag ttctcgccga agaacatgtg ccccgagatc tcgccgccga gcaggatgcc
      481 gtcctcgcgc atcttccgct tgaggagcga gtgcccggtc ttccacatga tgggctcccc
      541 gccgtgccgc cggatgtcgt ccaccagcac ctgcgacgac ttgacgtcga agatgatctt
      601 cgcccccggg tggcggacga gcaggtcgcg ggccagcagg gcgagcagca ggtccgcctc
      661 gtgccggcgg cccttctcgt ccacgatgcc gacccgatcc gcgtcgccgt cgtaggccag
      721 ccccacgtcg gcgcggacct cggtgacctt ggccaccagg tcccgcatat tctcctccat
      781 ctccggatcc ggaagatggt tggggaagtt gccgtcggac tcgcagtaca gctcgaccac
      841 ctcgcagccg agccgccgca gcagctcggg ggcgaagacg cccgcgatgc cgttgcccgc
      901 gtcgaccacc accttgaggc gccgcgcgag ccgcacgtgg ccggtgatgg cgcccagata
      961 ttcctcgcga ggatcgcggg cggtctggct tccgcggccc tgctcgaagt cgccggaccg
     1021 tatggtggcc agcaggccct ggatctcctc ctcggtgagc ggggccgccg ccgcgtgcac
     1081 catcttgacc ccgttgtcgg agaccgggtt gtggctgccg gtcacggtgg cgccgccgtc
     1141 gagcccccag tgggcggtcg cgaagtagag cagcggggtg tggttgaccc cgatgtcgac
     1201 cacgtcgagc ccggtcgcga ggaccccggc cccgaaggcc gccttgagcg cggccgacga
     1261 gacgcggttg tccatgccga gggccaccgt ccggcccccg cggcgcttca ccagggtgcc
     1321 gtacgcccgc cccacctgct cgaagacggg cggagtgatg tcgctgccga ccttgccgcg
     1381 cacgtcgtag gcgcggaaga tgtacgggtt cagctcggcc acgactccgg cctgcctaca
     1441 ggatggcgat cgcttcgatc tcgatcagcc actccggcat cgcgagggcc gccactccga
     1501 ccagggtcga ggcgcacgtc ttcttgccga ggaactcctc gcggatcgta cccaggtcgg
     1561 cgcgattgcg gatgtcggtg aggtaggtgt tgatcttgac gatgttgtcc atcgtgccgc
     1621 cgcccgcttc gacctgggcc ttgatcgcct ggaagacggc gcgggcctgc gccttgaagt
     1681 ccccgcggtg cgcgggcccg cccttgtcgt cgtaggcgac ctggcccgcg aggtagagaa
     1741 tggtctgggc tccggtgacc ttcacggcct gcgagtaggt cggcggatcc cagacaccct
     1801 tggcggcgta gcgctcgatc ttgaccatgc gatctcctct gaccgggaat gggctgctga
     1861 cggttgaggt actatagcgc gaaacggagg gatatcgcat ggacgacgcc ctgaaggaga
     1921 ccacgcgcaa gaccgcccgc atgctcttcc actcgatcaa gagcggcgtc gggttcgaca
     1981 cgtggaagaa gttcgacaag gatctggccc gcgagctcag catgttcttc accggcaagc
     2041 tctacagccg ggaggtcatc tcccagaagc agcgcgagct gtgcgcggtg gcctccctca
     2101 cggtgctgaa ccgccgcaac gaggtgcacg cccacattca cgccgcgctc aacgtggggg
     2161 cctcgcgcca ggaggtgacc gaggtcatct tccagcaggt gacctacggc gggatgccgg
     2221 tggtggtgga ggccctccag gtgttcaagg aagtgctcgt cgagcggggc gaatggcagg
     2281 actgaccgcg gccgtcccgg acgtcgtcac cgcgatgatc cgggccgccg aggcggccgg
     2341 cgaggtcgcc atgcggtact accgcggcgg cttcgaggtc accatcaagc cggaccagac
     2401 cccggtgacc caggccgacc gcgaggccga gcaggccatc aaggcgatcc tgcgacgcgc
     2461 gtgccccgat cacggcttcc tcggcgagga gttcggggag gaggggccga aggagcggcg
     2521 cttcatcatc gacccgatcg acggcaccaa gaacttcatc cggcgcgtgc cgatctgggc
     2581 ggtgctcatc ggcctcgagg aggcgggaca ggtcaccgcg ggagtggtgc tcaatccggc
     2641 gagcggcgag atgctctggg cgcaccgggg ggacggggcg tgggaccggg acgggcgcct
     2701 gcgcgtgtcc gactgcggcc ggctcgccga ggcgaccgtg ctccactccg acctgcatct
     2761 gctgcgccag ggcccccact gggacgcttt cttgcgaatg gtcgacggga gccgccgcag
     2821 ccgcggcttc ggcgactact acggctacgg cctggtcgcc gcgggcaagg ccgagctcta
     2881 cctggaggtg gatctcaagc cctgggacgt ggcgccgatg ctggtgctgc tcgaggaggc
     2941 gggcggccgg ctcaccgact tctcggggcg cgccaccatc tacgacggct cggtgctggc
     3001 caccaacggc cggcttcacg agcagatcct ggccctgctc cgtcggtagc ccggggccgg
     3061 tgcgactcga gccgctccga ctgatccgcc ccgcgccggc cctcgcggcc gccatcgcct
     3121 cgccgcccta cgacgtggtg agccgggccg aggccgccgc gctcgcgcgg gacaacccgc
     3181 actccttcct ccacatcggc cgcgccgaga tcgacctgcc cgacgaggtg gatccctacg
     3241 atcccctcgt ctacgtgcgc gcgcgcgaga acctggccgc gctgctcggg ccgggggcgc
     3301 tccggcgcga gccggggccc agcctcttcc tctaccggca ggtcatggaa gggcggaccc
     3361 aggtgggcgt ggtcggcggc gtgcgcatcg aggactacga gaccggcctc atccgcgagc
     3421 acgagaccac gcggccggac aaggaggccg atcgcacccg tcatctgctg gcgctcgacg
     3481 cgcaggccga gccggtgctg ctcgcctatc gcgggcgacc cgagatcgac cggctcgtgg
     3541 cggagacgct ggcgggcccg ccgctcgatg acttcaccac cggcgacggg gtcgcccagg
     3601 cggtctggcg ggtcgccgac gcggcgccgt acctgaccgc gttcgcctcg gtccaggcgg
     3661 cctacgtggc cgacggccac caccgctcgg ccgccgcggc gcgggcggcg gagaagcgcc
     3721 gcgccgagag ctcgcgcccg cgcggcgacc gcgcctacga gtggctgctc tcggtgctgg
     3781 tgcccgcggc ccagctccg
//
LOCUS       DATMCV010000973         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_253808, whole genome shotgun sequence.
ACCESSION   DATMCV010000973 DATMCV010000000
VERSION     DATMCV010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253808"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..822)
                     /locus_tag="VNU03_22205"
     CDS             complement(4..822)
                     /locus_tag="VNU03_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77253.1"
                     /translation="MRLDVADLAFGYPGKEVGRDVTFGLAGGEVLCLLGPNGGGKTTL
                     FKTVLRLLAPRGGRVAVDGEPIAGWSRGRLARVFGYVPQAQLGLFPFTVAETVLMGRT
                     AHIGPFATPSRGDRAVAEEMLAVLGIGHLAARPYTEISGGERQLTLIARALAQQPAIL
                     VMDEPTASLDFGNQVRVLGEIEQLARRGIAVMLSTHDPDHAFLCAHRVALLHRGRLAR
                     LGPPAEVITRESLREVYGIEVVVTEVPRADGRASRVCIPTLGTPPPLPSGERAG"
     gene            complement(826..1782)
                     /locus_tag="VNU03_22210"
     CDS             complement(826..1782)
                     /locus_tag="VNU03_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWP77254.1"
                     /translation="MLVAFSVGRYPVSPAQLLTLLWAKLTGAPTTLPAAMETVVFRVR
                     GPRVLAAVAIGATLAAAGSAYQGLFRNPLVSPDILGVSSGAALGATIGIYLSWGVAGI
                     QGLAFGLGLGAVAAVYAVGASLRRHDPILVLVLAGIVIGTLLGSCISLLKYLADPYNQ
                     LPAITFWLLGSLASITLVDLLSILPGVLVGLIPLWLLRWRMNVMTLSEEEALTLGVDT
                     RRVRLIVIGAATLMTAAVVSVSGVIGWIGLLIPHFARLLVGPDFSRLLPAAMLLGAGY
                     LVAVDTLARTIAPLEIPLGVLTAFIGAPFFIWLLAVSRRGWQ"
     gene            complement(1866..>2272)
                     /locus_tag="VNU03_22215"
     CDS             complement(1866..>2272)
                     /locus_tag="VNU03_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HWP77255.1"
                     /translation="ESLERLGARNVAEMPGGLAAVSPEQVLGWDPEIVIAIDPAFVAS
                     VRVDPLWKNVRAVRDGRVYLVPQAPFPWVDFPPSVNRLIGLKWLGRIFYPEQFPEDLR
                     QETRAFYTLFYHQAPTEAQLDTLLAGVERPRP"
BASE COUNT          374 a          803 c          860 g          235 t
ORIGIN      
        1 ccctcaccct gccctctccc cggaggggag agggggaggc gtgccgaggg tggggataca
       61 gacgcgggat gcgcggccat cggcgcgcgg cacctcggtc accaccacct cgatgccgta
      121 gacctcgcgc aggctctcac gggtgatcac ctccgcgggc gggcccagac gggccaggcg
      181 gccccggtgc agcagggcca cccggtgggc gcagaggaag gcgtggtccg ggtcgtgcgt
      241 ggagagcatc accgcgatgc cgcggcgggc gagctgctcg atctcgccga gcacgcgcac
      301 ctggttgccg aagtcgaggc tcgcggtggg ctcgtccatg accaggatgg ccggctgctg
      361 ggccagcgcc cgcgcgatca gggtgagctg ccgctcgccg ccgctgatct cggtgtaggg
      421 ccgcgcggcc aggtggccga tgccgagcac cgcgagcatc tcctccgcga ccgcgcggtc
      481 gccgcgcgac ggcgtggcga agggcccgat gtgcgcggtg cggcccatca gcacggtctc
      541 ggccaccgtg aacgggaaga gcccgagctg cgcctgcggc acgtagccga acacgcgcgc
      601 gaggcgcccg cgcgaccagc ccgcgatggg ctcgccgtcg accgcgacgc ggccgccgcg
      661 cggggcgagc aatcgcagga cggtcttgaa gagcgtggtc ttgccgccgc cgttcgggcc
      721 gagcaggcag agcacctcgc cgccggccag gccgaaggtc acgtcccggc ccacctcctt
      781 gcccggatag ccgaaggcca gatcggcgac gtcgaggcgc acggcctact gccacccccg
      841 gcgcgacacc gcgagcagcc agatgaagaa cggcgcgccg atgaacgcgg tgagcacgcc
      901 gagcgggatc tcgagcggcg cgatggtgcg ggcgagcgtg tccaccgcga ccaggtaacc
      961 ggcgcccagc agcatggcgg cggggaggag gcgcgagaag tccgggccca ccaggagccg
     1021 cgcgaagtgc gggatcagga gcccgatcca gccgatgacc ccactcaccg acaccaccgc
     1081 cgcggtcatc aaggtggccg ccccgatcac gatcagccgc acccgccgcg tgtccacgcc
     1141 cagggtgagg gcctcctcct cgctgagcgt catcacgttc atgcgccagc ggaggagcca
     1201 gagcgggatc aggccgacca gcacgccggg caggatcgag agcaggtcca ccagcgtgat
     1261 ggacgcgagg ctgcccagca gccagaaggt gatggccgga agctggttgt agggatcggc
     1321 caggtacttg agcagcgaga tgcaggagcc gagcagggtg ccgatgacga tgccggccag
     1381 caccagcacc aggatgggat cgtggcgtcg cagcgaggcg cccaccgcgt agaccgccgc
     1441 caccgcgccg agccccagcc cgaaggccag gccctggatg ccggccacgc cccacgagag
     1501 gtagatgccg atggtggcgc cgagcgcggc gccggaggag acgccgagga tgtcgggcga
     1561 gaccagcggg ttgcggaaga gaccctggta ggcggagccg gcggcggcca gcgtggcccc
     1621 gatggccacc gccgcgagca cgcgcggccc gcgcacgcgg aagaccaccg tctccatcgc
     1681 ggcgggcagc gtggtgggcg cgccggtgag cttggcccag agcagggtca agagctgcgc
     1741 gggcgagacc gggtagcgcc cgaccgagaa ggcgaccagc atgagggcca ccagcagcgc
     1801 gccggcgagc gcgaacccga ccacctcgcg gcggcggccc gcctcgacgg tcggggccgg
     1861 agcgctcagg gacgcgggcg ctcgaccccg gccagcaggg tgtcgagctg cgcctcggtc
     1921 ggcgcctggt ggtagaagag cgtgtagaag gcccgggtct cctgccgcag atcctccggg
     1981 aactgctcgg gatagaagat gcgcccgagc cacttgagcc cgatcaggcg attcaccgac
     2041 ggcgggaagt ccacccacgg aaacggggcc tgcgggacca ggtacacgcg gccgtcccgg
     2101 accgcgcgga cgttcttcca gagcgggtcg acgcgcaccg acgccacgaa ggcgggatcg
     2161 atcgcgatga cgatctccgg gtcccagccg agcacctgct cgggcgagac ggcggcgagg
     2221 ccccccggca tctcggccac gttgcgcgct cccaatcgct cgaggctctc ca
//
LOCUS       DATMCV010000974        10351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_519431, whole genome shotgun sequence.
ACCESSION   DATMCV010000974 DATMCV010000000
VERSION     DATMCV010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519431"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(548..1084)
                     /locus_tag="VNU03_22220"
     CDS             complement(548..1084)
                     /locus_tag="VNU03_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77256.1"
                     /translation="MRRGLVQSKRWLVVVALGAGVAAGCAAPLAGDIPAYPTASQLEV
                     DTQACEQAATGRAEFERRADYMACMISRGYRTYVTVATYWNLAELNVLAASKGKQAQA
                     QQAQSQVLLDLQTCATDAGAVAGARPLELAEAVDWVNVKVLRRGERMRDGALAGLVAE
                     CLGKRGYTARTPKRVASD"
     gene            complement(1110..1499)
                     /locus_tag="VNU03_22225"
     CDS             complement(1110..1499)
                     /locus_tag="VNU03_22225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77257.1"
                     /translation="MSLLSNVSTTSGRILVVDDDAGARDLIVSSLRADGHDVDVSSSG
                     IEALRLLGREWYDLIVSNLRMPELDGPSLYSAVTQRWPAQPPPLLFLAGLGGTSDYDG
                     FLKVIRAHVLPKPFTIRALRRIIRQML"
     gene            1729..5298
                     /locus_tag="VNU03_22230"
     CDS             1729..5298
                     /locus_tag="VNU03_22230"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HWP77258.1"
                     /translation="MEGTAATAVVRRERARRAVDLAEALLREARAGQTHDERGRAERL
                     ARLMEDPAGKELTIALTDQAFRSRRPERIADQLQYLLERYGAPRFMEWWERIGLTVGA
                     LMGQYMPSLVVPPIVARLRHETESLILPGEDADLRRYLAERRADGIRLNLNQLGEAIL
                     GEAEAARRLDAYLALLARDDVEYISVKVSSVSSQIDLTAFRHTVEVVKERLRTLYRQA
                     QRHHYRHPDGRVTPKFVNLDMEEYRDLDLTVAAFREVLDEDEFKALPAGIVLQAYLPE
                     SHRAQRVLVAWAMARVGRGGAPIKLRIVKGANLAMERIEADLHGWAEAPYAVKLEVDA
                     NFKRMIEYGSRREHARAVHLGVASHNLFDVAYVLLLRADLGIERWVEFEMLEGMANHQ
                     ARVVQARAGGLLLYAPVVRAEDFHSAIAYLVRRLDENTAPDNFLRHVFGLEPGSPDWE
                     LERDRFLQAFDRLDDLSDAPRRTQDRAAETGIAATDLAAPFVNEPDTDWALPANREWI
                     ERVREAWRERPAETVPLQIAGELITGGRQAVGRDRSRPGRVAYRHGLADRAQLDRALD
                     AARVAQAAWAARPPEERRALLDGAAAMLARRRGDLIGAMIVDGAKTVAEADPEVSEAI
                     DFARYYARALGVGDEVDGARMEPLGVVVVAPPWNFPLSIPAGGVLAALAAGNAVLLKP
                     APEAVLVGGHLARALWDAGIPREALQLVPLRDDEVGRDLITDPRVDGVILTGSVETAR
                     RFLDWRPDLHLFAETSGKNAIVVTALSDRDQAIRDLVRSAFGHDGQKCSAASLAICLA
                     EVYDDPAFRRQLRDATESLTVGRAWDEASRITPLTQPPGEALRRALTSLDPGEEWLLE
                     PREMSREAPLWSPGIKLGVRPGSFFHLTECFGPVLGLMRAADLDEAIALVNAIPFGLT
                     SGIQSLDDREIARWLDRVEAGNLYVNRHITGAIVARQSFGGWKASSVGPGAKAGGPNY
                     VAQLGHWHQVALPAAGAPLPPAMAELLERCFEAVPPGEPAAVLRASAASYAHAWQEHF
                     GREHEPVRLLGERNVFRYRGCRRVLVRAEAATPAGRLALAQVLLAARVCGVAVEVSLA
                     EGVPGPWLADSADVAVAVEPQLALAGRLGTGGVERLRALVPLSREVRAAAHAAGVAVL
                     DAPVLGAGRLELRAYLREQAVSRTVHRYGNVMQEKESSCASAS"
     gene            5280..6266
                     /locus_tag="VNU03_22235"
     CDS             5280..6266
                     /locus_tag="VNU03_22235"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77259.1"
                     /translation="MRIGVLIPTRAVVMESARRPPVEDCWTMARRADRAGYDAVWVGD
                     SIVAKPRLDALTTLAYLAGITDRVRLGTAVLLPALRHPVVLAQQLANVDQISRGRLLL
                     GLGVGWSLPSAEREWAACGADHKGRVRRLEEHVQIWRRLWTGEPVTHHGHDLDLVDHT
                     IGPLPWHPEGPPILITAGNRGELLPAQFDRFGRLGDGIITTYVHPEECGLIRERALEG
                     LARHGRARADFPLVVYTTVRLEDDVRTAERATTEFLTTYYGGGVHERGLMGLGPAEVV
                     VEALGRYAAAGVTDLCIRFVGADQLAQLDRFTAEVLPALQTTKPLPTEERAR"
     gene            6263..6706
                     /locus_tag="VNU03_22240"
     CDS             6263..6706
                     /locus_tag="VNU03_22240"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP77260.1"
                     /translation="MTEFPKLTYETLTVGEEFVSDTHLVTPEDIEAYAFAVEDEHPWF
                     SGPSPFGGPVAHPTLMANQALLLRHGKYIVHAGLHAKMEFNFLEPVRPGMRVRTRGRV
                     IDKYERRGRQYMVTEYHTEDEQGTPLVRGQFTQMLIPEGTRHAGR"
     gene            6693..7151
                     /locus_tag="VNU03_22245"
     CDS             6693..7151
                     /locus_tag="VNU03_22245"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP77261.1"
                     /translation="MPVAETIAVGQELPALRKEPTQRRIDVYSGVRPRSIHTDPSWAH
                     AKGFRTTLAQGMMSTAYVSEMMTRLLGAGFVKGGTLSMTFIKPVYAGDRLTVHGVVKE
                     TRREGAGTRVVVEVWCQNQDGEKTAVGTASGLAPHPPAPHPPAPHPTLSP"
     gene            complement(7163..8182)
                     /locus_tag="VNU03_22250"
     CDS             complement(7163..8182)
                     /locus_tag="VNU03_22250"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWP77262.1"
                     /translation="MHALVLERFGGPFVAKEVPTPAIAPHEALVRVRNVGVCGTDVKI
                     RANRMGLGVIPLIMGHEIAGEVAEVGAEVRGFAAGDRVVVNFYLTCGRCRFCRAGRDT
                     LCPETRQHGFSIDGGFAEYVKTPGVNLCKVPDHVSLEGACILGDAVATSYHAITRRAQ
                     VRPGTTVALVGVGGVGLHALQMARLAGAWVVAIDVSEARLELARSLGADAVIDARRGP
                     FHDAVRKLTDGQGVDVVLEFVANETTLPSSYQSVRRAGRLVFVGYTPQLPLPVMPHEL
                     VRNELEIVGSRANTKQELQETMDLVARGRIKPIVDRVIPLADVETAFDALREGRPLGR
                     NVLAV"
     gene            complement(8182..>10351)
                     /locus_tag="VNU03_22255"
     CDS             complement(8182..>10351)
                     /locus_tag="VNU03_22255"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWP77263.1"
                     /translation="AALKAPGVIAVLTGEDLAADGLGPLPTDGGRKRRDGSPAFRTPR
                     PALVSGRARHVGDPLALVVAETAAQAADAAELVTVDYEPLPAAVGAAAATAAGAPAVW
                     DEAPGNVAFVWEAGSREAVARGFAGAAHVTRLDFVVTRVAALPMEPRAAVGEFDRRTG
                     RYTLHTGIQAPHGLRTLLAEQVLRVPHTQVRVVTGDVGGSFGMRSGIYPELVLVLWAA
                     KRLGRPVKWTSSRRESFLSDEHGRDNVSSAELALDPGGKFLALRVAIKLDIGAYLTTR
                     SAGPGTNNVGGVAGVYTTPAIHVETTGVFTNTTPTGPYRGAGRPEATYAIERVIDIAA
                     GELGIDPLELRRRNLIPAAAMPFKTGLVFTYDCGEFERIMELAVTLGDRAGFERRRAD
                     ARGRGKLLGLGLANPIEVAGGPYTAVNPDTAEMRVNADGSVSLFAGSTSMGQGNETAF
                     AQIVADRLGLPPDRIQVFWGDSDALGAGRGNGGSGAISVGGSAVLRATEKIVERGRRI
                     AAHLLEAAPDDVALRDGRFTVAGTDRGVGWSQVAKTAYQPRQLPAGLEPGFSETAAFT
                     PPSVTFPNGCHVCEVEIDAETGAVRVTRYAVVDDVGRMINPMLVKGQIHGGIVQGLGQ
                     GLAEVLPYDASGQLLAGSLMDYAIPRADELPLFDVDSHEVATRVNPLGAKGVGEAGTV
                     GALPALLNAVNHALAPLGVRHLDMPVTPERVWQAMRANGH"
BASE COUNT         1360 a         3739 c         3849 g         1403 t
ORIGIN      
        1 gccccccggt tatctagcgg ttatcgctac ttggtcggcg ccggaagcgg cacgcccgga
       61 gtcgccgggt tgcacatgga gctacccggc cgccgcgcct gccccggagg gcactggacg
      121 cccggggtcg gatccggcat ccccggggtg gagctgccgg gcacgccggg ggtcgtgctg
      181 ccgggcatgc gcggcgtcgg ggcgccgggc gtgctggggg cggggggctt ggcgtgctcg
      241 gagacggaac gccaccggga gccggagtgc cgccgggcgg cggtgtctgc gccatcgccg
      301 gtccggccag gatcaaggtc gttgccgcta tcgtgaggga tgttttccag gtcatgagat
      361 tgctctcctt ccgggatctg gagggagcaa ggcgtgtgcc gcgggggatg atgcgggatt
      421 ccgcccgcta cgcaggcgat ggacggcggc gaccgagcga agtgggaaga gactcctcgg
      481 tgggggttac cgaggcgaga gagaaccgcc cctcaccctg ccctttcccc ggaggggaga
      541 gggggattta gtcggaggcg acgcgcttgg gggtgcgggc ggtgtagccg cgcttgccga
      601 ggcactcggc gacgaggccg gcgagggcac cgtcgcgcat acgctcgccg cgccggagca
      661 ctttcacgtt gacccagtcg accgcttccg ccagctcgag cggccgggcc cccgcgacgg
      721 cgcccgcgtc ggtggcgcac gtctgcagat cgaggagcac ctggctctgc gcctgctggg
      781 cctgcgcctg cttgcccttg cttgcggcca gcacgttcag ctcggccagg ttccagtagg
      841 tggccaccgt gacgtaggtg cgatagccgc gcgagatcat gcacgccatg tagtccgcgc
      901 gacgctcgaa ctcggcgcgg ccggtggcgg cctgctcgca cgcctgcgtg tccacctcga
      961 gctggctcgc ggtgggatag gcggggatgt cgccggccag cggcgccgcg catcctgccg
     1021 cgaccccggc acccagcgcg acgacgacca gccaccgctt cgactgcacg agacctcgcc
     1081 tcatcgatgc acctccgtcc gtctggcgtc tacagcatct ggcggatgat ccggcgcagc
     1141 gcccggatcg tgaagggctt cggcagcacg tgggcgcgga tcaccttgag gaagccgtcg
     1201 tagtcggacg tgccgccgag cccggccagg aacagcagcg ggggcggctg agcgggccac
     1261 cgctgcgtca cggcggagta gaggctcggg ccgtccagct ccggcatgcg gagattggac
     1321 acgatcaggt cgtaccactc gcggcccagc aggcgcagcg cctcgatccc gctgctgctc
     1381 acgtccacgt cgtggccgtc ggcgcgcagg ctcgagacga tcaggtcgcg ggcccccgcg
     1441 tcgtcgtcga cgacgaggat gcgtccggag gtggtgctga cgttcgatag gaggctcatg
     1501 gctcttggcg acgatgtcgt cgcgctcggt atgcattcgg tgcatcggag gtgccatggg
     1561 ttctcacctg tcttgacgcg gggttacacg gcgtagcctc ggggcgggtg tctttggcgt
     1621 gacggcgtgt ggggtaggaa ttacatgagg gccctcacgg gtgacagccc gacccctcac
     1681 cccaccccct ctcccccctg gggagagggg gtggggtaga ctgcggcgat ggaaggcacc
     1741 gccgccaccg ccgtcgtccg ccgggagcgt gcccggcgcg cggtcgatct ggcggaggcg
     1801 ctgctgcgcg aggcgcgggc cgggcagacc cacgacgagc gcgggcgggc ggagcgcctg
     1861 gcgcggctga tggaggatcc ggcgggcaag gagctgacca tcgcgctcac cgaccaggcc
     1921 ttccgcagcc ggcggccgga gcgcatcgcc gatcagctcc agtacttgct cgagcgctac
     1981 ggcgcgcccc gcttcatgga atggtgggag cgcatcgggc tcacggtggg cgcgctcatg
     2041 ggtcagtaca tgccgagcct ggtcgtcccg ccgatcgtgg cccgcctgcg gcacgagacc
     2101 gagtcgctga tcctgcccgg cgaggacgcg gacctgcgcc gctacctggc cgagcggcgg
     2161 gccgacggca tccggctcaa cctgaaccag ctcggcgagg ccatcctggg cgaggccgag
     2221 gcggcgcgcc gactcgacgc ctacctcgcg ctgctggccc gcgacgacgt cgagtacatc
     2281 tcggtcaagg tctcctcggt gtcgagccag atcgacctca ccgcgttccg ccacaccgtg
     2341 gaggtcgtga aggagcggct ccgcacgctc tatcgccagg cgcagcgcca tcactaccgg
     2401 cacccggacg ggcgggtcac gccgaagttc gtgaatctcg acatggagga gtaccgcgat
     2461 ctcgacctga ccgtcgccgc gttccgcgag gtgctggacg aggacgagtt caaggcgctg
     2521 ccggccggca tcgtgctgca ggcctacctg ccggagtcgc accgcgcgca gcgggtgctg
     2581 gtggcctggg cgatggcgcg cgtcgggcgc ggaggcgcgc cgatcaagct gcgcatcgtc
     2641 aagggcgcca acctggccat ggagcggatc gaggccgatc tgcacggctg ggccgaggcg
     2701 ccctacgcgg tgaagctcga ggtcgacgcc aacttcaagc gcatgatcga gtacggcagc
     2761 cgcagagagc acgcgcgcgc cgtgcactta ggcgtcgcga gccacaacct cttcgacgtg
     2821 gcctacgtgc tcctgctccg cgccgatctg ggtatcgagc gctgggtgga gttcgagatg
     2881 ctggagggca tggccaacca ccaggcgcgc gtggtgcagg cgcgggccgg gggcctgctg
     2941 ctctacgcgc cggtggtgcg ggcggaggac ttccacagcg cgatcgcgta cctggtgcga
     3001 cggctcgacg agaacaccgc gccggacaac ttcctgcgcc acgtgttcgg cctcgagcca
     3061 ggctcgcccg actgggagct cgagcgcgac cgcttcctcc aggccttcga ccgcctcgac
     3121 gacctgtcgg acgcgccgcg gcgtacccag gatcgcgcgg ccgagaccgg catcgcggcg
     3181 accgatctcg cggctccatt cgtgaacgag ccggacaccg actgggccct gcccgcgaat
     3241 cgcgagtgga tcgagcgggt ccgggaggcc tggcgcgagc gcccggcgga gacggtgccg
     3301 ctccagatcg cgggtgagct gatcacgggc gggcgccagg cggtgggccg cgaccgctcg
     3361 cgcccgggtc gcgtcgccta ccgtcacggg ctcgccgacc gcgcgcagct cgatcgcgcg
     3421 ctcgacgcgg cccgcgtcgc gcaggcggcg tgggcggccc gtccgcccga ggagcggcgc
     3481 gcgctcctcg acggcgccgc ggccatgctc gcccggcggc gcggcgacct gatcggcgcg
     3541 atgatcgtcg acggcgccaa gacggtggcc gaggcggatc cggaggtgtc ggaggcgatc
     3601 gacttcgcgc gctactacgc gcgtgccctc ggcgtgggcg atgaggtgga cggcgcccgc
     3661 atggagccgc tcggcgtcgt cgtggtggcg ccgccgtgga acttcccgct ctcgatcccg
     3721 gcgggcgggg tgctcgccgc gctggccgcc ggcaacgcgg tgctgctcaa gcccgcgccg
     3781 gaggcggtgc tggtcggcgg gcacctggcc cgcgcgctct gggacgcggg catcccgcgc
     3841 gaggccctcc agctcgttcc cctgcgggac gacgaggtcg ggcgcgacct gatcaccgat
     3901 ccgcgagtgg acggcgtcat cctgaccggc tcggtggaga ccgcgcggcg attcctcgac
     3961 tggcggcccg atctgcacct cttcgcggag acgagcggca agaatgcgat cgtcgtcacc
     4021 gcgctctcgg accgcgacca ggccatccgc gacctggtgc gctcggcctt cggccacgac
     4081 ggccagaagt gctcggcggc cagcctcgcc atctgcctag cggaggtcta cgacgacccg
     4141 gccttccgcc gccagctccg cgacgcgacg gagagcctca cggtcggccg cgcgtgggat
     4201 gaggcgagcc gcatcacgcc gctcacccag ccgcccggcg aggcgctccg ccgggccctg
     4261 acctcgctcg acccgggcga ggagtggctg ctcgagccgc gagagatgag ccgggaggcg
     4321 ccgctctggt cgcccggcat caagctcggg gtgcgcccgg gctcgttctt ccacctgacc
     4381 gagtgcttcg gcccggtgct cggcctcatg cgtgccgcgg atctcgacga ggccatcgcg
     4441 ctcgtcaacg cgatcccgtt cgggctcacg agcggcatcc agtcgctcga cgaccgcgag
     4501 atcgcgcgct ggctcgaccg cgtcgaggcc ggcaacctct acgtgaaccg ccacatcacc
     4561 ggggccatcg tggcgcgcca gtccttcgga ggctggaagg cgtcctcggt ggggccgggg
     4621 gccaaggccg gcgggccgaa ctacgtggcc cagctcggcc actggcacca ggtcgcgctg
     4681 ccggcggccg gcgcgccgct gccgcccgcc atggccgagc tgctggagcg ctgcttcgag
     4741 gcggtgccgc ccggagagcc ggcggcggtg ctgcgggcca gcgcggccag ctacgcgcac
     4801 gcctggcagg agcacttcgg tcgggagcac gagccggtcc ggctcctggg cgagcgcaac
     4861 gtcttccgct accgcgggtg ccggcgcgtg ctcgtgcgcg cggaggcggc gacgcccgcc
     4921 gggaggcttg ccctcgccca ggtcctcctg gccgcgcgcg tgtgcggggt cgcggtcgag
     4981 gtcagcctcg cggagggcgt gcccggcccc tggctggccg actcggccga cgttgcggtg
     5041 gcggtggagc cccagctcgc gctggccggg cggctcggga caggcggagt cgagcgcctg
     5101 cgcgcgctgg tgccgctctc gcgcgaggta cgggcggcgg cccacgccgc cggcgtggcg
     5161 gtcctcgacg cgccggttct cggggcgggg cgcctcgagc tgcgcgccta cctgcgcgag
     5221 caggcggtct cgcgcaccgt ccaccgctat ggcaatgtca tgcaggaaaa ggagagctca
     5281 tgcgcatcgg cgtcctgatt cccacgcggg cggtggtgat ggagtcggcc cggcgtccgc
     5341 ccgtcgagga ctgctggacc atggcccgcc gcgcggaccg ggccggctac gacgcggtgt
     5401 gggtgggtga cagcatcgtg gccaagccac ggctcgacgc gctgacgacg ctggcctacc
     5461 tggccggcat cacggaccgc gtgcggctcg ggaccgcggt gctgctgccc gcgctccgcc
     5521 acccggtggt gctggcccag cagctcgcca acgtcgacca gatctcgcgc ggccgcctgc
     5581 tgctcggcct gggcgtgggc tggagcctgc cgagcgccga gcgcgagtgg gcggcctgcg
     5641 gcgcggacca caaggggcgg gtgcgccggc tggaggagca cgtccagatc tggcggcggc
     5701 tctggaccgg cgagccggtg acccatcacg gccacgatct cgatctggtg gaccacacca
     5761 tcgggccgct gccgtggcat ccggaggggc cgcccatcct gatcaccgcg gggaaccgtg
     5821 gcgagctgct gccggcccag ttcgatcgct tcggccggct cggcgacggc atcatcacga
     5881 cctacgtgca ccccgaggag tgcgggctga tccgcgagcg ggccctcgag ggcctggcgc
     5941 gccacggccg cgcccgcgcc gatttccccc tcgtcgtcta caccacggtg cggctcgagg
     6001 acgacgtgcg caccgcggag cgcgccacca ccgagttcct caccacctac tacggcggcg
     6061 gcgtgcacga gcgggggttg atgggcctcg gccccgcgga ggtcgtcgtc gaggcgctcg
     6121 gccgctacgc cgcggcgggc gtgaccgatc tctgcatccg cttcgtcggc gcggatcagc
     6181 tcgcccagct cgaccgcttc accgcggagg tgctcccggc gctgcaaacg acgaaaccgt
     6241 tgcccacgga ggagcgagcg cgatgaccga gttcccgaag ctcacctacg agaccctgac
     6301 cgtcggcgag gagttcgtgt cggacaccca cctggtgacc ccggaggaca tcgaggccta
     6361 cgccttcgcg gtggaggacg agcatccctg gttctccggc ccgtcgccct tcggcggccc
     6421 ggtggcgcac ccgaccctca tggccaacca ggccctgctc ctccgccacg gcaagtacat
     6481 cgtgcacgcc ggcctccacg ccaagatgga gttcaacttc ctcgagccgg tgcggccggg
     6541 catgcgcgtg cgcacccgcg gccgggtgat cgacaagtac gagcggcggg gccgccagta
     6601 catggtcacc gagtatcaca ccgaggacga gcagggcacg cccctcgtcc gggggcagtt
     6661 cacccagatg ctgatcccgg agggcacccg ccatgccggt cgctgagacc atcgcggtcg
     6721 ggcaggagct gcccgcgctc cggaaggagc cgacgcagcg gcggatcgac gtctactcgg
     6781 gggtgcggcc gcgctccatc cacaccgacc cgtcctgggc gcacgccaag gggttccgca
     6841 ccacgctggc gcaggggatg atgtcgaccg cctacgtgtc ggagatgatg acgcggctcc
     6901 tgggtgccgg cttcgtcaag ggcggcacgc tctcgatgac gttcatcaag ccggtctacg
     6961 cgggcgaccg gctcaccgtg cacggggtgg tgaaggagac gcggcgcgag ggcgcgggca
     7021 cgcgggtggt cgtcgaggtg tggtgtcaga accaggacgg ggagaagacc gcggtgggga
     7081 ccgcgagcgg cctcgcccct caccctcccg cccctcaccc tccagcccct caccctaccc
     7141 tctccccgta ggggagaggg ttctatacag cgaggacgtt gcggccgagg gggcggccct
     7201 cgcggagggc gtcgaaggcg gtctcgacgt cggccagcgg gatcacccga tcgacgatgg
     7261 gcttgatgcg gccgcgggcc accaggtcca tcgtctcctg cagctcctgc ttggtgttgg
     7321 cgcgcgagcc cacgatctcg agctcgttgc gcaccagctc gtgcggcatc accggcagcg
     7381 gcagctgggg cgtgtagccc acgaagacca gccgcccggc gcgccgcacc gactggtagc
     7441 tcgacggcag cgtggtctcg ttggcgacga actccagcac cacgtcgacg ccctggccgt
     7501 cggtgagctt ccgcaccgcg tcgtggaagg ggccgcggcg cgcgtcgatc accgcgtcgg
     7561 cgcccaggct ccgcgccagc tcgagccgcg cctcgctcac gtcgatcgcg acgacccacg
     7621 cgccggccag ccgcgccatc tgcagcgcgt gcaggccgac cccgccgacg cccaccagcg
     7681 ccaccgtcgt gcccggccgc acctgcgccc gcctggtgat cgcgtggtac gacgtggcaa
     7741 ccgcgtcgcc caggatgcac gcgccctcga gcgacacgtg gtccggcacc ttgcagaggt
     7801 tgacgcccgg ggtcttcacg tactcggcga acccgccgtc gatgctgaag ccgtgctgac
     7861 gcgtctcggg gcagagcgtg tcgcgccccg cccggcagaa ccggcagcgg ccgcaggtga
     7921 gatagaagtt caccaccacg cggtcgcccg ccgcgaagcc gcgcacctcc gcgcccacct
     7981 ccgccacctc gcccgcgatc tcgtggccca tgatcagcgg gatcacgccc agccccatgc
     8041 ggttggcgcg gatcttcacg tcggtgccgc agacgcccac gttgcgcacc cggaccagcg
     8101 cctcgtgcgg cgcgatggca ggcgtgggca cctccttggc gacgaagggc cccccgaagc
     8161 gttcgagaac cagcgcgtgc atcagtggcc gttggcccgc atggcctgcc agacccgctc
     8221 gggggtcacc ggcatgtcga ggtggcggac ccccagcggg gccagcgcgt ggttgaccgc
     8281 gttgagcagc gcgggcagcg cgcccaccgt gccggcctcc cccacgccct tggcgccgag
     8341 cgggttgacc cgggtcgcca cctcgtgcga gtccacgtcg aagagcggca gctcgtcggc
     8401 gcgcgggatc gcgtagtcca tcagcgagcc ggccagaagc tggcccgagg cgtcgtaggg
     8461 gagcacctcg gccagcccct ggcccaggcc ctggacgatg ccgccgtgga tctgcccctt
     8521 caccagcatc gggttgatca tgcggcccac gtcgtcgacc accgcgtagc gcgtcacgcg
     8581 gaccgcgccg gtctccgcgt cgatctcgac ctcgcagacg tggcagccgt tggggaacgt
     8641 caccgagggc ggcgtgaagg cggccgtctc gctgaaaccg ggctcgagcc cggccggcag
     8701 ctggcgcggc tgataggcgg ttttcgcgac ctggctccag cccaccccgc ggtcggtgcc
     8761 ggccaccgtg aaccggccgt cgcgcagggc cacatcgtcg ggggcggcct cgagcaggtg
     8821 cgcggcgatg cggcgcccgc gctcgacgat cttctcggtg gcgcgcagca ccgcggagcc
     8881 gccgaccgag atggcgcccg agccgccgtt gccgcggccg gcgccgagcg cgtcgctgtc
     8941 gccccagaag acctggatgc gatcgggcgg caggccgagg cggtcggcca cgatctgggc
     9001 gaaggcggtc tcgttgccct ggcccatcga cgtcgagccc gcgaagagcg agaccgagcc
     9061 gtccgcgttg acgcgcatct cggcggtgtc gggattgacc gcggtgtagg ggccgcccgc
     9121 gacctcgatc gggttggcga gacccaggcc gaggagcttg ccgcggcccc gcgcgtccgc
     9181 ccgtcgccgc tcgaagccgg cgcgatcgcc gagggtcacc gccagctcca tgatccgctc
     9241 gaactcgccg cagtcgtagg tgaagacgag gccggtcttg aacggcatcg cggccgccgg
     9301 gatcaggttg cggcggcgca gctcgagcgg gtcgatgccg agctcgcccg ctgcgatgtc
     9361 gatcactcgc tcgatcgcgt aggtcgcctc gggccggccg gcgccgcggt agggaccggt
     9421 cggggtggtg ttggtgaaga cgccggtggt ctcgacgtgg atggcgggcg tggtgtagac
     9481 gccggccacc ccgcccacgt tgttggtgcc gggccccgcg ctccgcgtcg tgaggtaggc
     9541 gccgatgtcg agcttgatgg cgacccgcag ggccaggaac ttcccgcccg ggtccagcgc
     9601 cagctcggcg gaggagacgt tgtcgcgccc gtgctcgtcg gagaggaagc tctcgcgccg
     9661 gctcgacgtc cacttcaccg gccggccgag gcgcttcgcg gcccagagca ccagcaccag
     9721 ctcggggtag atgccgctgc gcatcccgaa gctgccgccc acgtcgccgg tcaccacccg
     9781 cacctgggtg tgcggcacgc ggaggacctg ctcggcgagc agggtgcgga ggccgtgggg
     9841 cgcctggatc ccggtgtgga gcgtgtagcg cccggtgcgg cgatcgaact cgcccaccgc
     9901 cgcccgcggc tccatcggca gcgcggccac gcgcgtgacc acgaagtcga ggcgcgtgac
     9961 gtgcgcggct cccgcgaagc cgcgcgcgac cgcctcccgc gagccggcct cccagacgaa
    10021 ggccacgttg ccgggcgcct cgtcccagac cgcgggcgct ccggccgcgg tggcggccgc
    10081 cgcgccgacg gcggcgggca gcggctcgta gtcgaccgtg accagctccg cggcgtcggc
    10141 ggcctgcgcc gccgtctcgg ccaccaccag cgcgagcgga tcgcccacgt ggcgcgcccg
    10201 cccgctcacc agggcggggc gcggggtccg gaaggcgggc gagccgtcgc ggcgcttgcg
    10261 tccgccgtcg gtgggcaggg ggccgagccc gtccgcggcc aggtcctccc cggtgaggac
    10321 cgcgatcacg ccgggcgcct tcagcgccgc c
//
LOCUS       DATMCV010000975         2526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_652427, whole genome shotgun sequence.
ACCESSION   DATMCV010000975 DATMCV010000000
VERSION     DATMCV010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652427"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VNU03_22260"
     CDS             complement(<1..164)
                     /locus_tag="VNU03_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014780.1"
                     /note="catalyzes the fumarate and succinate
                     interconversion; fumarate reductase is used under
                     anaerobic conditions with glucose or glycerol as carbon
                     source; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HWP77264.1"
                     /translation="MPTFTMRVFRGSPEAGDFKEYKVEGGEGLVVLDVIHRIQATQAP
                     DLACRWNCKAG"
     gene            complement(164..1966)
                     /locus_tag="VNU03_22265"
     CDS             complement(164..1966)
                     /locus_tag="VNU03_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HWP77265.1"
                     /translation="MATEEYETHEHDVLVIGAGGAGLRAAIEAAAQGMSVGLVCKSLL
                     GKAHTVMAEGGIAAAMGNVWPEDNWEVHFRDTMRGGKMLNNWRMAQLHAQESPERVLE
                     LERYGALFDRTKEGLISQRDFGGHRYARLAHVGDRTGLEMIRTLQYHAIHQPIEVYME
                     CKIERLIKDGERVAGAFGYWRNTGKFVLFKAKAVVLATGGIGKAWKFTSNSWEYTGDG
                     HSLAVWAGADLIDMEFVQFHPTGMVWPPSVRGILVTEGVRGDGGTLKNTNGERFMFNY
                     IPEFFKGETADSQEEADRWYVDKKNNRRTPDLLPRDEVARAINSEVKAARGTPHGGVF
                     LDICTRRDADYIRKRLPSMYHQFKELAGVDITKEPMEVGPTCHYVMGGVRVDADSTAA
                     TVPGLFAAGEVAGGMHGSNRLGGNSLGDLLVFGRRAGLHAALYAKNLGGALKVDAGQV
                     EQIARQMLEPFTRTGGENPYTIQSDLQDTMQNLVGIIRVEAELKEALQKLEALKERAK
                     RVRVEGGRVYNPGWHTALDLASLLTVSECCALAALERKESRGGHTREDHPYTDDTWGT
                     VNLVSRQKGDRMELRREPLPQMPPELKALLQEKK"
     gene            complement(1966..>2526)
                     /locus_tag="VNU03_22270"
     CDS             complement(1966..>2526)
                     /locus_tag="VNU03_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HWP77266.1"
                     /translation="PDFTFPIIGVVSPAFLILGGPGLFRLTCYYYRKAYYRSFWLAPA
                     ACAVRDAKSGYTGETRFPLILQNFHRYTWYVAVLFIVMLTWDAILAFRFKDGFGIGVG
                     TIVMWINVLFLAGYTFSCHSCRHVCGGAVDVFSKAPVRYSLWRGITRLNEKHPVFAWL
                     SLFSVGLTDLYIRLVSIGIIRDWRII"
BASE COUNT          380 a          862 c          864 g          420 t
ORIGIN      
        1 cccgccttgc agttccagcg gcaggcgagg tccggggcct gggtggcctg gatccggtgg
       61 atcacgtcga ggaccacgag gccctcgccg ccctcgacct tgtactcctt gaagtcgccc
      121 gcctcggggc tgccgcggaa cacccgcatc gtgaaggtcg gcatcacttc ttctcctgga
      181 ggagggcttt caactcgggc ggcatctggg gcagcggctc gcgccgcagc tccatccgat
      241 cccccttctg ccgggagacc aggttgacgg tgccccaggt gtcgtcggtg tacggatgat
      301 cctcgcgggt atggccgccg cggctctctt tccgctcgag ggcggcgagc gcgcaacact
      361 ccgagacggt cagcaatgag gccaggtcga gcgcggtgtg ccagcccggg ttgtacacgc
      421 gcccgccctc gacgcgcacc cgcttcgcgc gctccttcag ggcctcgagc ttctggagcg
      481 cctccttcag ctcggcctcg acccggatga tcccgaccag gttctgcatc gtgtcctgca
      541 ggtcggactg gatcgtgtac gggttctcgc cgccggtccg ggtgaagggc tcgagcatct
      601 gacgcgcgat ctgctccacc tggcccgcgt ccaccttgag ggcgccgccc aggttcttgg
      661 cgtagagcgc ggcgtgcaga cccgcgcgcc ggccgaacac caggaggtcg ccgagggagt
      721 tgcccccgag ccggttgctg ccgtgcatgc cgcccgcgac ctcgccggcc gcgaagaggc
      781 cgggcacggt ggccgcggtc gagtccgcgt cgacccgcac cccgcccatc acgtagtggc
      841 aggtgggacc cacctccatc ggctccttgg tgatgtcgac gccggccagc tccttgaact
      901 ggtgatacat ggagggcagg cgcttgcgga tgtagtcggc gtcgcgccgc gtgcagatgt
      961 cgaggaacac cccgccgtgg ggcgtgcccc gggccgcctt cacctccgag ttgattgcgc
     1021 gcgcgacctc gtcgcgcggc agcaggtcgg gagtgcggcg gttgttcttc ttgtcgacgt
     1081 accagcggtc ggcctcttcc tggctgtccg cggtctctcc cttgaagaac tcggggatgt
     1141 aattgaacat gaaccgctcg ccgttggtgt tcttgagcgt gcccccgtcg ccccggaccc
     1201 cctcggtgac caggatgccg cgcaccgagg gcggccacac catcccggtc gggtggaact
     1261 ggacgaactc catgtcgatg aggtcggcgc cggcccagac cgcgagcgag tggccgtcgc
     1321 cggtgtactc ccaggagttc gaggtgaact tccacgcttt gccgatgccg ccggtggcca
     1381 ggacgaccgc cttggccttg aagagcacga acttgccggt gttgcgccag tagccgaagg
     1441 ctcccgccac ccgctcgccg tccttgatca gccgctcgat cttgcactcc atgtagacct
     1501 cgatcggctg atggatcgcg tggtactgga gcgtgcggat catctcgagg ccggtgcggt
     1561 cgcccacgtg ggccaggcgc gcgtagcggt ggcccccgaa gtcgcgctgg gagatcaggc
     1621 cctccttggt gcgatcgaag agggcgccgt agcgctccag ctccagcacc cgctccgggc
     1681 tctcctgggc gtgcagctgg gccatgcgcc agttgttgag cattttgccg ccgcgcatgg
     1741 tgtcgcggaa gtgcacctcc cagttgtcct ccggccacac gttgcccatc gcggccgcga
     1801 tgccgccctc ggccatgacg gtgtgggcct tgccgaggag cgacttgcag acgagaccga
     1861 ccgacatgcc ctgcgccgcc gcctcgatgg ccgcgcgcag cccggcgccg cccgcgccga
     1921 tgacgaggac gtcgtgctcg tgtgtctcgt actcttcggt cgccatcaga tgatcctcca
     1981 gtcgcggatg atcccaatgg agacgaggcg gatgtagagg tccgtcagcc ccacgctgaa
     2041 cagggacagc caggcgaaga ccggatgctt ctcgttgagc cgggtgatgc cgcgccacag
     2101 cgagtagcgc accggggcct tggagaagac gtccaccgcc ccgccgcaga cgtgccggca
     2161 ggagtggcag gagaacgtgt agccggccag gaacagcacg ttgatccaca tcacgatggt
     2221 gcccaccccg attccgaagc cgtccttgaa ccggaaggcg aggatcgcgt cccaggtgag
     2281 catcacgatg aacagcaccg ccacgtacca ggtgtagcgg tggaagttct ggaggatgag
     2341 cgggaagcgg gtctcgccgg tgtaccccga cttggcgtcc cgcaccgcgc aggccgccgg
     2401 ggccagccag aacgagcggt agtaggcctt gcggtagtag tagcaggtca ggcggaacag
     2461 gcccgggccg cccaggatga ggaacgcggg cgacaccacc ccgatgatcg ggaacgtgaa
     2521 gtccgg
//
LOCUS       DATMCV010000976         3754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_519642, whole genome shotgun sequence.
ACCESSION   DATMCV010000976 DATMCV010000000
VERSION     DATMCV010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519642"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VNU03_22275"
     CDS             complement(<1..608)
                     /locus_tag="VNU03_22275"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP77267.1"
                     /translation="MLDLKITGGQLIDGTGAPARRGDVGVTGDAITAVGDLAREPAGR
                     TIDASGRTVTPGFIDMHSHSDWRLWDNRRAESKIRQGVTTEVVGNCGFSPAPVSDQFR
                     AEMKGFALYLPAGMDFAWKSVREYLAHYEGEGVALNVAQLIGHGTLRLAAMGFARRPP
                     TAGEQAHMERMMDEAMADGAYGLATGLIYAPGSYAATEEIIG"
     gene            complement(619..1710)
                     /locus_tag="VNU03_22280"
     CDS             complement(619..1710)
                     /locus_tag="VNU03_22280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWP77268.1"
                     /translation="MASELDDKFQTLHEIVRAARMNLAPGPWDYMVGGTETETTLRRN
                     RLGIDSLAFRPRVLRDVSRIDTGATFFGKRLRIPVMMAPVGSIETFTPGGGATAAKAS
                     AAFGVPQMLSSACNPGLEATAAAADNFRIFQLYVRGDDAFVDDHVKRAKDHGYPVFCM
                     TVDTAMYSRRERDLARRFVKPWRLRATGQDFQAALSWDQVKRFKDRHDLPLILKGIAT
                     EEDAAIACDHGVDGVYVSNHGGRQLDHGRGSIEVLPEVVKAVAGRSTIIVDGGFMRGT
                     DVLKAIALGAQVVGLGRLTCLGLAAAGEAGLVRTLELLEDEIRIAMGLLGVHSLDMLD
                     ASYLHPATPVTVPHVTSAFPLLDLDDRRY"
     gene            complement(1735..2196)
                     /locus_tag="VNU03_22285"
     CDS             complement(1735..2196)
                     /locus_tag="VNU03_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77269.1"
                     /translation="MKSKRPRKPARSKRPASKRRPVPARSPRDPLLAPMAGASHRVIG
                     GVTIDIMEAANGRVKRVCYPVGFRWSTHMKPIVKTPLCMHAHVGFLARGHVRGTYADG
                     CEFDVSAPRAVVLDPGHDAWVVGREPAVLIEFDALGQTAERFGLPPSHQHA"
     gene            2283..3185
                     /locus_tag="VNU03_22290"
     CDS             2283..3185
                     /locus_tag="VNU03_22290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77270.1"
                     /translation="MPALLDARSLVKHLGGRRVVDGVDLGCETSQIVGLLGPNGAGKT
                     TTLRMLYGFLRPDAGQIHVNGVELGRDLVRAKRAIGVCTQDDTFDGDFTVEQNLSIAA
                     RYFRPRPTDLARRVAELLERFDLATYAREKPETLSGGYRRRLMIARALVHRPRLLFLD
                     EPTTGLDPQARMGVWDLVDGLRAEGLGIILTTHYMDEAERLSDALTVLARGRVVARGT
                     AKTVLGDLVGEHVVVVDAGQGAAVAAWLAARGRGEPASVLGDLHLPLSGEELAEFARA
                     FPALRYEVRGPTLDDLFLKLSLAP"
     gene            3182..>3754
                     /locus_tag="VNU03_22295"
     CDS             3182..>3754
                     /locus_tag="VNU03_22295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77271.1"
                     /translation="MSWLAAALEWQSRAVVGRHLRVYMRNWHTAFLPPAVEPVTMLVA
                     FGVGLGGYVATLTWQGRPIEYMTYVAPGLLAYATFMTAIFQSLFAAFIRMRYQRTWEG
                     QLTTQVELTHVVWGEVLWAGLLATMYVVIVSAVLVVIQWAGLLALDLRWLPVLWPIAF
                     VAGCGFAALGLCFTAIVPTIDHMNLPVFLLV"
BASE COUNT          489 a         1283 c         1403 g          579 t
ORIGIN      
        1 atgccgatga tctcctcggt cgccgcgtag gagccggggg cgtagatgag gccggtggcg
       61 aggccgtagg cgccgtcggc catcgcctcg tccatcatgc gctccatgtg cgcctgctcg
      121 cccgcggtgg gcggccggcg cgcgaagccc atggcggcca ggcgcagggt gccgtggccg
      181 atgagctgcg ccacgttcag cgcgacgcct tctccctcgt agtgggcgag gtactcgcgg
      241 accgacttcc acgcgaaatc catccccgcg ggcaggtaga gagcgaagcc cttcatctcg
      301 gcgcggaact gatccgagac cggagcgggg gagaagccgc agttgccgac cacctcggtg
      361 gtcacgccct ggcggatctt cgactcggcg cgccggttgt cccagagacg ccagtccgaa
      421 tgcgagtgca tgtcgatgaa gccgggcgtc acggtgcgcc cgctcgcgtc gatggtgcgc
      481 ccggccggct cgcgggcaag atcgccgacc gcggtgatgg cgtcgccggt cacccccacg
      541 tcgcctcggc gcgcgggcgc gccggtgccg tcgatgagct gcccgcccgt gatcttgagg
      601 tcgagcacgg gcgcgggcct agtagcgccg gtcgtcgaga tccagcagcg ggaacgcgct
      661 ggtgacgtgc gggacggtga ccggagtggc cggatgcaga tacgaggcgt cgagcatgtc
      721 gaggctgtgc accccgagca ggcccatggc gatgcggatc tcgtcctcca ggagctcgag
      781 cgtccggacg aggcccgcct cgccggccgc ggcgaggccg aggcaggtca ggcgccccag
      841 gcccaccacc tgcgcgccga gcgcgatggc cttcagcacg tcggtgccgc gcatgaagcc
      901 gccgtccacg atgatggtgg agcgcccggc caccgccttc accacctccg gcagcacctc
      961 gatcgagccg cggccgtggt cgagctggcg cccgccgtgg ttggagacgt agaccccgtc
     1021 cacgccgtga tcgcaggcga tggccgcgtc ctcctcggtg gcgatgccct tcaggatcag
     1081 cgggaggtcg tgacggtcct tgaagcgctt cacctggtcc cacgagagcg cggcctggaa
     1141 gtcctgcccg gtggcgcgca ggcgccacgg cttgacgaag cgccgggcca gatcccgctc
     1201 gcggcggctg tacatcgcgg tgtccaccgt catgcagaag acggggtagc cgtggtcctt
     1261 cgcgcgcttg acgtggtcgt ccacgaaggc gtcgtcgccg cgcacgtaga gctggaagat
     1321 gcggaagttg tccgcggcgg ccgcggtggc ctcgaggccg ggattgcagg ccgagctcag
     1381 catctgcggc acgccgaagg cggcggaggc cttggccgcg gtggcgccgc cgcccggggt
     1441 gaaggtctcg atcgatccga ccggggccat catcaccggg atgcgcaggc gctttccaaa
     1501 gaaggtcgcg ccggtgtcga tgcgcgagac gtcgcggagc actcgcggcc ggaaggccag
     1561 gctgtcgatg ccgaggcgat tgcgccggag cgtggtctcg gtttcggtgc cgcccaccat
     1621 gtagtcccac gggccggggg ccaggttcat tcgggcggcg cggacgatct cgtgcagcgt
     1681 ctggaacttg tcgtcgagct cggaagccat ggtctctgtc gtctccggat cgggtcaggc
     1741 gtgctggtgg ctggggggca ggccgaagcg ctcggcggtc tggccgagcg cgtcgaactc
     1801 gatgagcacc gcgggctcgc ggccgacgac ccacgcgtcg tggccggggt cgagcacgac
     1861 cgcgcgcggg gcggacacgt cgaactcgca gccgtccgcg taggtgccgc gcacgtgccc
     1921 gcgggcgagg aatcccacgt gggcgtgcat gcagagcggc gtcttcacga tcggcttcat
     1981 gtgggtggac cagcggaagc cgaccgggta gcagacgcgc ttcacccgtc cgttggccgc
     2041 ttccatgatg tcgatggtga cgccgccgat cacccggtga gaggcgcccg ccatgggggc
     2101 caggagcgga tcgcgcgggc tccgcgcggg cacgggacgt cgcttcgacg cggggcgctt
     2161 ggagcgggcc ggtttccgcg ggcgcttgct cttcatctgc tctgtctcct gggcgcgacg
     2221 tgggacgtcg agacactgac tcccgcggcc ggggcttgtc aagtccggta gactaggacg
     2281 ctatgcctgc tctgctcgac gctcgcagtc tggtcaagca cctcggcggt cgccgcgtgg
     2341 tggacggggt ggacctcggc tgcgagacct cgcagatcgt gggcctgctc gggcccaacg
     2401 gcgcgggcaa gaccacgacg ctccggatgc tatacggctt cctgcgcccg gacgcgggcc
     2461 agatccacgt caacggggtg gagctggggc gcgacctggt gcgggccaag cgcgcgatcg
     2521 gggtgtgcac gcaggacgat accttcgacg gcgacttcac ggtggagcag aacctctcca
     2581 tcgcggcgcg ctacttccgc ccgcggccga ccgatctcgc ccgacgcgtg gccgagctcc
     2641 tggagcgctt cgacctggcg acctacgcgc gggagaagcc ggagacgctc tcgggcggct
     2701 accggcgccg gctgatgatc gcccgcgcgc tggtgcaccg cccgcgcctc ctcttcctcg
     2761 acgagcccac caccggcctc gacccgcagg cgcgcatggg ggtgtgggat ctcgtcgacg
     2821 gcctgcgcgc cgagggcctc ggcatcatcc tgaccacgca ctacatggac gaggcggagc
     2881 ggctgagcga cgcgctcacc gtgctggcgc gcggccgcgt ggtggcgcgc ggcaccgcca
     2941 agacggtgct gggcgacctg gtgggcgagc acgtggtggt ggtggacgcg gggcagggcg
     3001 ccgcggtcgc ggcctggctc gccgcgcgcg ggcgaggaga gccggcgagc gtactgggcg
     3061 acttgcacct gccgctctcc ggtgaggagc tggccgagtt cgcgcgcgcg ttccccgcgc
     3121 tgcgctacga ggtgcgcggg cccacgctcg acgacctctt cctcaagctc tcgctcgcgc
     3181 catgagctgg ctggccgcgg cgctcgagtg gcagagccgc gcggtggtgg gccgccacct
     3241 gcgcgtctac atgcgcaact ggcacaccgc gttcctgccg cccgcggtgg agccggtcac
     3301 catgctggtc gcgttcgggg tcgggctcgg cggctacgtg gcgacgctca cctggcaggg
     3361 ccgcccgatc gagtacatga cctacgtggc gccgggcctg ctcgcttacg cgaccttcat
     3421 gaccgcgatc ttccagtcgc tcttcgccgc cttcatccgc atgcgctacc agcgcacctg
     3481 ggagggccag ctcaccaccc aggtcgagct gacccacgtg gtgtggggcg aggtgctctg
     3541 ggcgggcctg ctcgcgacca tgtacgtggt catcgtgtcc gcggtgctgg tggtgatcca
     3601 gtgggcggga ttgctcgcgc tcgatctgcg gtggctgccg gtgctgtggc cgatcgcctt
     3661 cgtggccggc tgcgggttcg cggccctcgg gctctgcttc accgcgatcg tgccgacgat
     3721 cgaccacatg aacctgccgg tgttcctgct cgtg
//
LOCUS       DATMCV010000977         4387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_120231, whole genome shotgun sequence.
ACCESSION   DATMCV010000977 DATMCV010000000
VERSION     DATMCV010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120231"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VNU03_22300"
     CDS             <1..1008
                     /locus_tag="VNU03_22300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77272.1"
                     /translation="TLQCLAGLVRPDAGRIVVDGHVLFDSVAGIDVRPQRRRVGYVFQ
                     GYALFPHLTVRGNVGFGLRDRGRVDRRRREDEVLARLGLEAFAERYPRELSGGQRQRV
                     ALGRALATDPALLLLDEPLSALDLPLRQALRDELRSVLTGWGIAAVLVTHDLTEAYRL
                     GDRIVVYEEGRVIQAAPRAELLSQPSSRSVARIMGIRNLVQGVTLKATADRIQLRWRG
                     QVLEAVNSPTRSYLPAPDSPLSFFIRPEYVRLIRKDRAGVDGAHHMNLMSGTVVREVD
                     LGTSWTLFLRLDEPGTPAQGDYDLELEVPRLVYEILEIERDRHWQFSLHRGAIHVLPS
                     A"
     gene            1008..2102
                     /locus_tag="VNU03_22305"
     CDS             1008..2102
                     /locus_tag="VNU03_22305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77273.1"
                     /translation="MAPPPVVALHGIQVAHAGRTVLSIPELAVDAGQVLAVIGPNGAG
                     KSTLLRVMGLLETPTAGSVRFQGERVRPADGLAIRRRMASVFQDPLLTDATVGDNVAL
                     GLRFRGVDRERVAPRVTEWLEQLGIAHLASRQARTLSGGEAQRTALARALVLEPELLL
                     LDEPFSALDQPTREALIEDLGRILQRRRATAVLVTHDRGEAQALGDRVAVMLEGRLLQ
                     VDEAGQVFRAPVSEEVARFVGVETLLECRVQAVTAGLAILEAGKQTIEVAQPAEAGEW
                     VRLCVRPEDVTLTARAGGSASSARNHLEGQIVRLAQSGPHVRVTIDCGFPLVALVTQR
                     SVTEMGLGPGSPVTAHFKATAAHLLRHAKP"
     gene            2213..2443
                     /locus_tag="VNU03_22310"
     CDS             2213..2443
                     /locus_tag="VNU03_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HWP77274.1"
                     /translation="MPDTEAFIERLTAENHDFRKARDEHRQYEIELSDLNGRGFLSPD
                     QQWRVSELKKLKLIAKDRMESLLRHARAATHV"
     gene            2483..3016
                     /gene="moaC"
                     /locus_tag="VNU03_22315"
     CDS             2483..3016
                     /gene="moaC"
                     /locus_tag="VNU03_22315"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWP77275.1"
                     /translation="MPGFRGLSHLTPAGEARMVDVSAKAETVREAAARVTLKMKAATL
                     AAVRAGQLAKGDVLGVARTAGIMAAKRTPDLIPLCHPLRITGVDVEFALDSRRSTITV
                     LARVRTVDKTGVEMEALTAAAVAGLTVYDMVKAVDKGIVLTDLCLVEKSGGKSGPWKR
                     RGLARRSRRRAERIQDS"
     gene            3013..4143
                     /locus_tag="VNU03_22320"
     CDS             3013..4143
                     /locus_tag="VNU03_22320"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HWP77276.1"
                     /translation="MTTTAIVYSDEWRHFDYGREHPLRMERLGLTWRLMEAYGLTALP
                     GAKVLTPEVATLEEIARFHTREYIDILRAVSAGDWVPTAARYGLGPGDNPIFPGLWEA
                     AQLGAGGSLLAAQVVATGEADRAFHFAGGLHHAMPGRASGFCYVNDAVLAIMRLRARG
                     FRVAYVDIDAHHGDGVQFAFYDDPNVLTVSTHERGDRLFPGTGFVAEMGEGDGLGYAV
                     NLPLQPLTDDSVYEPAFEAVVPPLVTAFRPDALVIQLGIDSHRTDPLTHLSLTVQGFT
                     RAVRRLLPLAPRVVALGGGGYDLANVARAWTAAWAAMNDVELPDALPPESHPDMQRLG
                     LARPTLSDPPETQPAETRRWAEEYARRQVGEIQDRIFPLHGL"
     gene            4150..>4387
                     /locus_tag="VNU03_22325"
     CDS             4150..>4387
                     /locus_tag="VNU03_22325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77277.1"
                     /translation="MSVEFPASGASVPAVTEPAAPPPEHAAIRVPLKVKLSLLITALV
                     VLAVALVGLFLLRQQQQSLTAEMTKRGLTMAENFA"
BASE COUNT          592 a         1607 c         1589 g          599 t
ORIGIN      
        1 acgctgcagt gcctggccgg gctcgtgcgc cccgacgcgg gccgcatcgt ggtcgacggc
       61 cacgtgctct tcgattcggt ggccgggatc gacgtgcggc cccagcggcg gcgcgtcggt
      121 tacgtcttcc agggctacgc gctcttcccg cacctcacgg tgcggggcaa cgtgggcttc
      181 ggcctccgcg accgcggccg ggtcgaccgg cgtcggcggg aagacgaggt gctggcgcgc
      241 ctcgggctcg aggcgttcgc ggagcgctac ccgcgcgagc tgtcgggcgg ccagcgccag
      301 cgggtggccc tcggccgcgc gctcgcgacc gaccccgcgc tgctcctgct cgacgagccg
      361 ctctccgcgc tcgacctgcc gctgcgccag gcgctgcgcg acgagctgcg cagcgtcctc
      421 accggctggg gcatcgcggc ggtgctggtc acccacgacc tgaccgaggc ctaccggctc
      481 ggcgaccgca tcgtggtcta cgaggagggg cgcgtgatcc aggccgcgcc gcgcgccgag
      541 ctcctgtcgc agccctcgtc gcgcagcgtg gcgcgcatca tggggatccg gaacctggtg
      601 cagggggtca cgttgaaggc caccgccgac cgcatccagc tccggtggcg cggccaggtg
      661 ctcgaggcgg tcaactcgcc gacgcgctcg tacctgcccg cgccggacag cccgctctcg
      721 ttcttcatcc ggcccgagta cgtgcgcctc atccgcaagg atcgcgccgg ggtggacggc
      781 gcccatcaca tgaacctcat gtccggcacc gtggtgcgcg aggtggacct gggcacctcg
      841 tggacgctct ttctgaggct cgacgagccg ggcacccccg cgcagggcga ctacgacctc
      901 gagctggagg tgccgcggct cgtctacgag atcctcgaga tcgagcgcga tcggcactgg
      961 cagttctccc tccaccgcgg cgcgatccac gtgctgccgt cggcctgatg gcgccgcccc
     1021 cggtggtcgc gctgcacggc atccaggtcg cgcacgcggg gaggaccgtg ctgagcatcc
     1081 ccgagctcgc ggtggacgcg ggccaggtgc tggccgtgat cggccccaac ggagccggca
     1141 agtcgacgct cctccgcgtg atggggctgc tcgagacgcc gaccgcgggc agcgtgcgct
     1201 tccaggggga gcgggtgcgc ccggcggacg gtctcgcgat ccgccggcgc atggccagcg
     1261 tcttccagga cccgctcctc accgacgcga ccgtcgggga caacgtggcg ctcgggctcc
     1321 gcttccgcgg cgtggaccgc gagcgcgtgg cgccccgggt gaccgagtgg ctcgagcagc
     1381 tcggcatcgc gcacctcgcc tcccgccagg cccgcaccct gtccggcggc gaggcgcagc
     1441 gcaccgccct ggcccgggcg ctggtgctag agcccgagct gctgctgctc gacgagccct
     1501 tctcggccct ggatcagccg acgcgggagg cgctgatcga ggacctcggg cggatcctgc
     1561 agcgccgccg ggccaccgcg gtgctggtga cgcacgatcg cggcgaggcc caggcgctcg
     1621 gggaccgggt ggcggtgatg ctcgaggggc ggctcctcca ggtggacgag gcgggccagg
     1681 tatttcgcgc gccggtctcg gaggaggtgg cgcgcttcgt cggcgtcgag accctgctgg
     1741 agtgccgggt ccaggcggtg accgccggcc tcgcgatcct cgaggccggc aagcagacca
     1801 tcgaggtggc gcagcccgcc gaggccggcg agtgggtgcg gctctgcgtg cggccggagg
     1861 acgtgacgct caccgcgcgg gccggcggca gcgcatcgag cgcgcggaat cacctggagg
     1921 gccagatcgt gcgcctggcc cagagtggcc cgcatgtgcg cgtgaccatt gactgcggat
     1981 tccccctggt ggcactggtg acgcagcgct ccgtcaccga gatggggctc gggcccggca
     2041 gcccggtgac cgcccacttc aaggccaccg cggcgcatct gctccgccac gccaagcctt
     2101 gacagccgaa agagcccggg agtataacgc ggataccgac gctggcaggg acggggccct
     2161 gaggagccgg aacagcctgg caagcccgga gcagcctcgt aaggaggtcc gcatgcccga
     2221 cacggaagcg ttcatcgaac gtctcaccgc tgaaaaccac gatttccgca aggcccgcga
     2281 cgaacaccgc caatacgaga tcgagttgtc cgacctgaat ggccgtggct tcctctcccc
     2341 tgatcaacag tggcgtgtga gcgagctcaa gaagctcaag ctcatcgcga aagaccggat
     2401 ggagagtctc ctccggcacg cgcgcgccgc gacccacgtc tgagtcgggt ccagcacccg
     2461 accgatgtgc ccgccgcggg atatgcccgg gttccgcggc ctctcgcacc tgacccccgc
     2521 gggtgaggcg cggatggtgg acgtctccgc caaggcggag accgtccggg aggcggccgc
     2581 ccgcgtcacc ctcaagatga aggcggccac cctcgccgcc gtccgcgcgg gccagctcgc
     2641 caagggcgac gtgctcggag tggcgcgcac cgcgggcatc atggcggcca agcgcacccc
     2701 ggacctgatc ccgctctgcc acccgctgcg catcacggga gtcgacgtgg agttcgcgct
     2761 cgactcgcgg cgctcgacga tcacggtgct cgcgcgcgtg cgcacagtgg acaagaccgg
     2821 ggtggagatg gaggcgctca ccgcggcggc ggtggccggc ctcaccgtgt acgacatggt
     2881 gaaggcggtc gacaagggta tcgtcctcac cgacctctgc ctcgtcgaga agtcgggcgg
     2941 caagtccggc ccgtggaagc gtcgtggcct ggcgaggcgc agccgcaggc gagccgagcg
     3001 gatccaggat tcgtgaccac caccgcgatc gtctactcgg acgagtggcg ccacttcgac
     3061 tacgggcgcg agcatccgct ccgcatggag cggctcgggc tcacgtggcg gctgatggaa
     3121 gcctacggcc tcaccgcgct gccgggcgcg aaggtgctca ccccggaggt ggccaccctc
     3181 gaggagatcg cccgcttcca cacccgcgag tacatcgaca tcctgcgcgc ggtgagcgcg
     3241 ggggactggg tgccgaccgc cgcccgctac ggcctcgggc ccggcgacaa cccgatcttc
     3301 cccgggctgt gggaggcggc ccagctcggc gcgggcggct ccctgctcgc ggcccaggtg
     3361 gtggccaccg gcgaggccga tcgcgcgttc cacttcgcgg gcgggctcca ccacgcgatg
     3421 ccggggcgcg cctccggctt ctgctacgtc aacgacgcgg tgctggccat catgcggctg
     3481 cgcgcgcgcg ggttccgggt cgcctacgtg gacatcgacg cccaccacgg cgacggggtg
     3541 cagttcgcgt tctacgacga tccgaatgtg ctcaccgtgt ccacccacga gcgcggcgat
     3601 cgcctcttcc ccggcaccgg cttcgtggcg gagatgggcg agggggacgg cctcggctac
     3661 gcggtgaacc tgccgctcca gccgctcacc gacgactcgg tgtatgagcc ggcgttcgag
     3721 gcggtggtgc cgccgctggt gaccgccttc cggcccgacg cgctcgtgat ccagctcggc
     3781 atcgactcgc accgcaccga tccgctcacc cacctgagcc tcaccgtgca gggcttcacg
     3841 cgcgcggtgc gccggctcct gccgcttgcc ccgcgcgtcg tcgcgctcgg tggcggcggc
     3901 tacgatctcg ccaacgtggc ccgggcctgg accgcggcgt gggcggccat gaacgacgtg
     3961 gagctgccgg acgcgctgcc gccggagagc caccccgaca tgcagcgcct cggcctcgcg
     4021 cggccgacgc tcagcgatcc gcccgagacg cagcccgcgg agacgcgccg ctgggccgag
     4081 gagtacgcgc gccgccaggt gggcgagatc caggaccgga tcttccccct ccatggactg
     4141 taggccggcg tgagcgtgga gttccccgcg agcggagcga gcgtcccggc cgtgaccgag
     4201 ccggcggcgc cgccgccgga gcacgccgcg atccgggtgc cgctcaaggt caagctgagc
     4261 ctgctcatca ccgcgctcgt ggtgctggcg gtggccctgg tcggcctctt cctcctgcgc
     4321 cagcagcagc agagtctcac cgccgagatg accaagcgcg ggctcaccat ggccgagaac
     4381 ttcgcgg
//
LOCUS       DATMCV010000978         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_786463, whole genome shotgun sequence.
ACCESSION   DATMCV010000978 DATMCV010000000
VERSION     DATMCV010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786463"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..641
                     /locus_tag="VNU03_22330"
     CDS             162..641
                     /locus_tag="VNU03_22330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP77278.1"
                     /translation="MGAKGETLAKQFEAKAHEAGVYIEKLGEADLEKVTAAEKWTVAA
                     TAHHLFGAYARVPDIARGLAAGQSPANFSTKVLDQMNAQHAKDFAACSKADLMALHHA
                     GSAKAASVLRGLSDEELGKSGVIFQDAPPFTVEQFVTAALIGHTDDHMGSIRKTVGK"
     gene            complement(667..1170)
                     /locus_tag="VNU03_22335"
     CDS             complement(667..1170)
                     /locus_tag="VNU03_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77279.1"
                     /translation="MLLAVALSVAQVAEAADGGVQVTLVAPGGPVTLTEPEQARIAKR
                     VEVLMVGCAISSVTEPDLFAKRSLPAEWQAARAGSHLYVRFPKPLVGTRGRVDISEVV
                     IGLQEPSFIGPDLSRHGEAVVGHIKCNGHRALALMCGTALRPHLRPGQAASCKVYDTI
                     GEPPETE"
     gene            complement(1369..>2138)
                     /locus_tag="VNU03_22340"
     CDS             complement(1369..>2138)
                     /locus_tag="VNU03_22340"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWP77280.1"
                     /translation="GRYSRQTSYISPPGDFTAAVAMPVGKLTDDWYFVCGVDVLASPE
                     TGGIVALGDSLTDANISTIDAFCRWPDQLSRRLQARAGGRPMAVMNQGLGGNRILHDL
                     RGDSGLRRFDRDALAQPGVTHVIVMLGTNDLRNRWAKPEEEVTAEQMIAGLKQLAVRA
                     RTRGIKIFGATLTPYENETFLVGAWTPEREAKRQAVNAWIREGGAFDAVVDFDEALRD
                     PVHPARMLPIYDCGDHLHPSDLGYNAMGDAIDLALFD"
BASE COUNT          335 a          716 c          764 g          323 t
ORIGIN      
        1 tcggggacgc cgtgggcggg tgacggggcc cactggcgtc caccagcgaa cctctcgtcc
       61 tgacgcagca ggtcgtaatc cacgctcggt gtcgatttgg gcgcccgttg tccgactatg
      121 gggtgggagc aggaaggtca cacgctaaaa aggaggcaga catgggagcc aaaggcgaga
      181 cgctggcaaa acagttcgag gcgaaggccc atgaggccgg agtgtatatc gagaagctag
      241 gtgaggcgga tctcgagaag gtaaccgcgg ccgagaagtg gacggtcgcc gcaacggcgc
      301 atcacctctt cggggcgtac gcccgcgttc ccgacatcgc cagggggctc gccgctgggc
      361 agtcgccggc caacttctcg acgaaggtgc tggaccagat gaacgcccag cacgcgaagg
      421 atttcgccgc gtgcagcaag gcggacctca tggcgctgca tcacgcggga tccgccaagg
      481 ccgcctcggt gctgcgcggg ctgagcgacg aggagctcgg caagagcggc gtgatcttcc
      541 aggacgcgcc gccgttcacg gtggagcagt tcgtgacggc cgccctcatc ggccacaccg
      601 acgaccacat ggggagcatc aggaagacgg tcggcaagta gggacggtcg agcaggcccg
      661 ggatcgctac tcggtctccg gcggctcgcc gatcgtgtcg tagaccttgc agctcgcggc
      721 ctggcccggc cggagatgag gccggagcgc ggtcccgcac atcagggcga gcgctcggtg
      781 gccgttgcac ttgatatggc cgaccaccgc ctcgccgtgc cgggagaggt cggggccgat
      841 gaagctcggc tcttgcagcc cgatgaccac ctcggagatg tccacgcgcc cgcgcgtccc
      901 cacgaggggc ttcgggaagc ggacgtacag gtgggagccg gcgcgggcgg cctgccactc
      961 ggcgggtagc gacctcttcg cgaagagatc gggctccgtc accgaggaga tcgcgcagcc
     1021 gaccatcagc acctcgacgc gcttcgcgat ccgggcctgc tccggctcgg tcagcgtgac
     1081 cgggccgccc ggcgcgacga gcgtgacctg gacgcccccg tcggcggctt ccgccacctg
     1141 cgccacggag agagcgacgg cgaggagcac cggcctcagg accagaaccg atcctcccgc
     1201 tgctcgtcgc cctgatactc gcggaactcg gtcacccgtc cgttctggag gcgccagacg
     1261 tgcacttccg gggcacgttg cccttcgcga aatcctcgta ggccctccgg atgatcgcgc
     1321 cactcgactc gcccatggtt cggctccttt ccgtcgccaa gtgatgcgtc agtcgaacag
     1381 cgccaggtcg atcgcgtccc ccatcgcgtt gtaccccaga tcgctcgggt ggaggtggtc
     1441 gccgcagtcg tagatcggca gcatgcgggc cgggtgcacc ggatcccgca gcgcctcgtc
     1501 gaagtcgacc accgcgtcga aggcgccccc ctcgcggatc cacgcgttga cggcctggcg
     1561 cttcgcttcg cgctccgggg tccatgcccc gacgaggaag gtctcgttct cgtacggcgt
     1621 cagcgtggcg ccgaagatct tgatgccgcg cgtcctggcg cgcaccgcga gctgcttcag
     1681 gccggcgatc atctgctcgg cggtgacctc ttcttcgggt ttcgcccagc ggttccggag
     1741 gtcgttggtg ccgagcatca cgatgacgtg ggtgacgccg ggctgggcca gcgcgtcgcg
     1801 atcgaagcgg cgcaggccgc tgtcgccccg gaggtcgtgc aggatgcggt tgccgccgag
     1861 cccctggttc atgaccgcca tcggccggcc gccggcgcgg gcctgtagcc ggcgcgagag
     1921 ctgatcgggc cagcggcaga acgcgtcgat cgtcgagatg ttggcgtcgg tcagcgagtc
     1981 gccgagggcg acgatgccgc ccgtctccgg tgaagccagc acgtcgaccc cgcagacgaa
     2041 gtaccagtcg tcggtgagct tgccgaccgg catcgcgacc gcggcggtga agtcgcccgg
     2101 cggggagatg tagctcgtct ggcgtgagta gcggccgg
//
LOCUS       DATMCV010000979         4853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_990199, whole genome shotgun sequence.
ACCESSION   DATMCV010000979 DATMCV010000000
VERSION     DATMCV010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990199"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VNU03_22345"
     CDS             complement(<1..793)
                     /locus_tag="VNU03_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP77281.1"
                     /translation="MKHRQVYRTELTPVSFLVRTAYVFPDKTAVVHGARRYTYRELGE
                     RVNRLAAALRGAGMRKHERVAFLCPNIPPMLEAHYGVPAAGGVLVAINTRLSSDEIGY
                     ILQHSGSTFLFVDAELEALIKPLDLGGVRVVRVDDTGAAGDPYEDFLAGGSPEAVESW
                     LEDEEETISINYTSGTTGRPKGVMYSHRGVWVNAVGEIVETAMTFDTKYLWTLPMFHC
                     NGWCFTWAVTAVAGTHVCLRKVDPGRIWDLIDAEGITHYNGAPTVQ"
     gene            889..2433
                     /locus_tag="VNU03_22350"
     CDS             889..2433
                     /locus_tag="VNU03_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWP77282.1"
                     /translation="MAWDERMKDFHARRATARGMGGPERLARRRAAGALNARERVARL
                     LDPDSFLEIGTFNTSDVPGMEAETPADSKVSGFGRIDGRPVVVCSNDFTVLAATSSRV
                     ASYKEAELKRLAARRGLPVVYLAEAGGARMPDIMGSAGIASFGHSTYYGTRRRRVPMV
                     TAVLGQAYGLPTWNACLSDFVVMLEGASMAVSGPRVLELATGEKISPEELGGSKLHAE
                     ETGFADAVGTTEDECLALVKRVLSYLPSHNRQAPPAAEVPAGSGAEMGTIADLVPEAR
                     NRAYDMTRVLRRLADSGEIFQVKERFGRSLITALARMGGQVVGFVANQPIQKGGACDA
                     DGCDKATSFLALCDSFNIPLVFLHDIPGFFIGRDAERKRVAAKIITWMEALGMVSVPR
                     VSIIVRKTYGQAYFNMGGGGYADLLVAWPTAEMSFMDPGTGVNVVHGAELAKLPAEAR
                     AGRRQALLAQWELDTLPYGAAGRHLVHDVIDPADTRDVVIRFLEQSRTRTAIGRHRLA
                     GWPTKF"
     gene            2445..3179
                     /locus_tag="VNU03_22355"
     CDS             2445..3179
                     /locus_tag="VNU03_22355"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWP77283.1"
                     /translation="MSLGPLSVTWLFGAAVIAGACFVQGLAGFGIGLVSLAFLPLLMS
                     PADAIVLITLYATVFTVVIFFPLRRDFTFHGMGELIVGSIIATPAGVFILAIMPPAAL
                     TRLIGLVLLVIVGLEWLGLYPELRGRGWGLGAGLAAGLLGGAIGTPGPPVILYAAAQD
                     WSPRTVKANIQAFLIVNQAVILIGYWWAGLLDREVWRLTWLYAAPAVAGLAAGMLLFN
                     RLDRARFRRVVFAVLFVSGLVLLIRG"
     gene            complement(3186..3545)
                     /locus_tag="VNU03_22360"
     CDS             complement(3186..3545)
                     /locus_tag="VNU03_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWP77284.1"
                     /translation="MTTPEALLERTYAAFNARDVDAVFAMMHAEVDWPNGMEGGRVHG
                     HRAVREYWTRQWGLIDPRVEPRGFTTEPDGRVAVDVRQVVRDRTGALLKDEMVRHVYR
                     IEDGLIRSMEIRPGSSA"
     gene            complement(3542..3994)
                     /locus_tag="VNU03_22365"
     CDS             complement(3542..3994)
                     /locus_tag="VNU03_22365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP77285.1"
                     /translation="MRAVIERVSEAPADRLGALVAESEQQGFGFVRRLVDEWTAGANR
                     FDRPGEVLFVARVGGDVVGVCGLNVDPHADDPKIGRVRHLYVLVRQRRSGIGEQLVAD
                     TIEAARGRFERLHLRTTNLTAARLYERLGFRRTGEGRDRTHVLHLISR"
     gene            complement(4006..4422)
                     /locus_tag="VNU03_22370"
     CDS             complement(4006..4422)
                     /locus_tag="VNU03_22370"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWP77286.1"
                     /translation="MTFDDPSIQQFLSTKDIVILGTVNPDGAPLVTAMWFLHDPSALT
                     LISMDGLQKVHNLRRDPRLHILAESGARGADIKGVAIRGRAEFLSDTPERRALATRIL
                     DKYQPHLERYWGGRAMPADRVMFRVVPQQVRTWGPD"
     gene            complement(4419..>4853)
                     /locus_tag="VNU03_22375"
     CDS             complement(4419..>4853)
                     /locus_tag="VNU03_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77287.1"
                     /translation="LVIAQLRKIGIRGTMKTHESAAGYAAFGKGDFTIIASQDRAMDI
                     NEPSGVFHISYTTDSGSNYGQYSDPKVDELTERALKETNRDKRKQVYWELQRYLLARG
                     DHASIAVGWVEGWFFKDKKLRNYKPGLTTYDNNTFMKTWIAP"
BASE COUNT          629 a         1735 c         1720 g          769 t
ORIGIN      
        1 tctgcacggt gggcgcgccg ttgtagtggg tgatcccctc cgcgtcgatc aggtcccaga
       61 tgcggcccgg gtcgaccttc cggaggcaga cgtgggtgcc ggccaccgcg gtgaccgccc
      121 aggtgaagca ccagccgttg cagtggaaca tgggcagggt ccagaggtac ttcgtgtcga
      181 aggtcatcgc ggtctcgacg atctcgccca ccgcgttcac ccacaccccg cggtgcgagt
      241 acatgacgcc cttgggccgc ccggtggtgc ccgaggtgta gttgatcgag atcgtctcct
      301 cctcgtcctc gagccagctc tcgaccgcct ccggcgagcc gccggccagg aagtcctcgt
      361 agggatcgcc cgccgcgccg gtgtcgtcga cccgcaccac ccgcaccccg ccgaggtcga
      421 gcggcttgat cagggcctcc agctccgcgt ccacgaacag gaaggtcgag cccgagtgct
      481 gcaggatgta gccgatctcg tcggaggaga gccgcgtgtt gatcgccacc agcacgccgc
      541 ccgccgcggg cacgccgtag tgcgcctcga gcatcggcgg gatgttgggg cagaggaagg
      601 cgacccgctc gtgcttgcgc atcccggcgc cacgcagcgc cgcggccagc cggttcacgc
      661 gctcgccgag ctcccggtac gtgtaccgcc gcgcgccgtg caccaccgcg gtcttgtcgg
      721 gaaagacgta ggcggtgcgg acgaggaagc tcacgggcgt gagctcggta cggtagacct
      781 gacggtgctt catcgcgagc ggcctccggg tgagcgggga tgaaaacgtg cctccgcagt
      841 gtacccaagc gcttccggaa tggcgagggg ctgctacaat gccccgctat ggcctgggac
      901 gagcggatga aggatttcca cgcccggcgc gcgaccgcgc gcggcatggg cgggcccgag
      961 cggctggcgc gccgccgggc cgcgggcgcg ctcaacgcgc gcgagcgggt ggcgcgcctg
     1021 ctcgacccgg actcgttcct cgagatcggc acgttcaaca cctctgacgt gccgggcatg
     1081 gaggccgaga cgccggccga ctccaaggtg agcgggttcg ggcgcatcga cggccgcccg
     1141 gtggtggtgt gctccaacga cttcacggtg ctcgcggcca cctcgagccg cgtggcctcg
     1201 tacaaggaag ccgagctcaa gcgcctggcc gcccgccgcg gcctgcccgt cgtctacctg
     1261 gccgaggcgg gcggcgcgcg catgcccgac atcatgggct cggcgggcat cgcctccttc
     1321 ggccactcca cgtactacgg cacccggcgc cggcgcgtgc cgatggtgac cgcggtgctc
     1381 ggccaggcct acgggctgcc gacctggaac gcctgcctct ccgacttcgt ggtgatgctc
     1441 gagggcgcct cgatggcggt gtcgggcccg cgggtgctcg agctcgccac cggggagaag
     1501 atctcgccgg aggagctggg cggatcgaag ctccacgccg aggagaccgg cttcgcggac
     1561 gcggtgggca cgaccgagga cgagtgcctc gcgctcgtga agcgcgtgct ctcctacctg
     1621 ccctcgcaca accggcaggc gccgcccgcc gcggaggtcc cggccggctc cggcgcggag
     1681 atggggacca tcgcggacct ggtgccggag gcgcgcaacc gggcctacga catgacgcgg
     1741 gtgctgcggc ggctggctga cagcggcgag atcttccagg tcaaggagcg cttcggccgg
     1801 agcctcatca ccgcgctcgc gcggatgggc ggccaggtgg tcggcttcgt ggcgaaccag
     1861 ccgatccaga agggcggcgc ctgcgacgcg gacggctgcg acaaggccac ctcgttcctc
     1921 gcgctctgcg actcgttcaa catcccgctc gtcttcctcc acgacatccc gggcttcttc
     1981 atcgggcggg acgcggagcg caagcgcgtg gcggccaaga tcatcacctg gatggaggcg
     2041 ctcgggatgg tctcggtgcc gcgcgtctcg atcatcgtgc gcaagaccta cggccaggcc
     2101 tacttcaaca tggggggcgg cggctacgcg gacctgctgg tggcgtggcc caccgccgag
     2161 atgtcgttca tggatccggg caccggggtg aacgtggtcc acggggcgga gctcgccaag
     2221 ctccccgccg aggcgcgtgc ggggcgccgc caggcgctgc tcgcccagtg ggagctcgac
     2281 accctgccct acggcgcggc gggccggcac ctcgtccacg acgtgatcga tccggccgac
     2341 acgcgcgacg tcgtcatccg cttcctcgag cagtcccgga cgcgcaccgc catcggccgg
     2401 catcggctgg ccggctggcc gaccaagttc taggcgcgcc ctcgttgtcg ctcggcccgc
     2461 tgtcggtgac gtggctcttc ggcgcggcgg tcatcgcggg cgcctgcttc gtccagggac
     2521 tggccggctt cggcatcggc ctggtctcgc tcgccttcct gccgctgctc atgtcgcccg
     2581 ccgacgcgat cgtgctgatc acgctctacg ccaccgtgtt caccgtcgtc atcttcttcc
     2641 ccctgcgccg cgacttcacg ttccacggca tgggcgagct gatcgtgggc tccatcatcg
     2701 ccacgccggc cggcgtcttc atcctcgcga tcatgccgcc cgcggccctg acgcgactca
     2761 tcggcctggt gctgctggtg atcgtggggc tcgagtggct cgggctctat ccggagctgc
     2821 gcggccgggg ctggggcctg ggggcggggc tggccgcggg cctgctcggg ggcgcgatcg
     2881 gcacccccgg cccgccggtg atcctctacg cggccgcgca ggactggagc ccgcggaccg
     2941 tgaaggccaa catccaggcc ttcctgatcg tcaaccaggc ggtgatcctg atcggctact
     3001 ggtgggccgg gctcctcgac cgggaggtct ggcggctcac ctggctctac gcggcgcccg
     3061 cggtggccgg cctcgccgcg ggcatgctcc tcttcaaccg gctcgaccgc gcgcgcttcc
     3121 gccgggtggt cttcgcggtg ctcttcgtct cgggcctggt gctgctgatc cgcggctgag
     3181 ggcgctcagg cgctggagcc ggggcggatc tccatcgacc ggatcaggcc gtcctcgatc
     3241 cggtagacgt gccgcaccat ctcgtccttg agcagcgcgc cggtccggtc gcgcaccacc
     3301 tggcgcacgt ccaccgccac ccggccgtcg ggctcggtgg tgaagccccg cggctccacg
     3361 cgcggatcga tgaggcccca ctgccgcgtc cagtactcgc ggaccgcgcg gtggccgtgg
     3421 acgcggccgc cctccattcc gttgggccag tccacctccg cgtgcatcat cgcgaacacc
     3481 gcgtcgacgt cgcgcgcgtt gaacgcggcg taggttcgct cgaggagggc ctcgggcgtc
     3541 gtcaccgcga gatcagatgc agcacgtggg tccggtcccg cccctcaccg gtgcggcgga
     3601 accccagccg ctcgtagagc cgcgccgcgg tgaggttggt ggtgcgcagg tgtagccgct
     3661 cgaaccggcc ccgggcggcc tcgatcgtgt ccgcgaccag ctgctcgccg atcccgctcc
     3721 gccgctggcg aaccagcacg tacagatgcc gcacccggcc gatcttcggg tcgtccgcgt
     3781 gcgggtccac gttcagcccg cacaccccga cgacgtcgcc gcccacccgc gccacgaaca
     3841 gcacctcgcc cggccggtcg aagcgattgg cgcccgcggt ccactcgtcg acgaggcggc
     3901 ggacgaagcc gaatccctgc tgctcgctct ccgcgaccag cgcgccgagc cggtcggccg
     3961 gcgcctcgct cacccgctcg atgaccgcac gcatcgcgcg agcggtcagt ctggccccca
     4021 cgtgcgcacc tgctggggca ccacgcggaa catcacgcgg tccgccggca tcgcgcgccc
     4081 gccccagtac cgctcgaggt ggggctggta cttgtcgagg atgcgggtgg cgagggcccg
     4141 ccgctccggg gtgtcgctca ggaactcggc gcggccgcgg atcgcgacgc ccttgatgtc
     4201 ggcgccgcgg gcgccgctct ccgcgaggat gtggagccgc gggtcgcgcc tcaggttgtg
     4261 gaccttctgc agcccgtcca tgctgatcag ggtgagcgca gacgggtcgt ggaggaacca
     4321 catcgcggtg acgagcggag ccccgtcggg gttgacggtg ccgaggatga cgatgtcctt
     4381 cgtggacaga aactgctgga tgctcggatc gtcgaaggtc acggggcgat ccaggtcttc
     4441 atgaacgtgt tgttgtcgta ggtggtgagg ccgggcttgt agttgcggag cttcttgtcc
     4501 ttgaagaacc agccctccac ccacccgacc gcgatcgagg cgtgatcgcc gcgggcgagc
     4561 aggtagcgct gcagctccca gtagacctgc ttgcgcttgt cccggttggt ctccttgagc
     4621 gcgcgctcgg ttagctcgtc gaccttggga tccgagtact ggccgtagtt gctccccgag
     4681 tcggtggtgt aggagatgtg gaacaccccg ctcggctcgt tgatgtccat cgcccggtcc
     4741 tgggaggcga tgatcgtgaa gtcccccttg ccgaaggcgg cgtagcccgc cgcgctctcg
     4801 tgggtcttca tcgtcccgcg gatgccgatc ttgcggagct gggcgatcac gag
//
LOCUS       DATMCV010000980         5065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_120249, whole genome shotgun sequence.
ACCESSION   DATMCV010000980 DATMCV010000000
VERSION     DATMCV010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120249"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VNU03_22380"
     CDS             <1..749
                     /locus_tag="VNU03_22380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWP77288.1"
                     /translation="ERAEHALRLARDLFEVRAVAQKEVRDAESEYRKTAAERDRAAAR
                     LRNLGVDPARTDGATTVAVTAPRSGVVVERNVTPGQVVAYGQSDLPSSLVVIADLSTM
                     WVLADVYEPDVPRVRLGEAVSVTLPCCPGERYQGQVASIGDAIDKESRTLKVRAVVPN
                     RGRALKSEMFARVSIDTGTRQALVVPQGAIHRDGSATFVLVEKTRGEYERRPVTVGAE
                     ADGVVEIRDGLTPADTVVSAGGILLKRSAR"
     gene            746..3829
                     /locus_tag="VNU03_22385"
     CDS             746..3829
                     /locus_tag="VNU03_22385"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWP77289.1"
                     /translation="MIERIVAFALHQRFVVLALTLILTVGGIVSYGHLPIEAYPDVGD
                     VKVEIITLWPGHAAEEVERLITIPLENELNGIANVTFLRSDTLFGLSNIRVLFADGTD
                     DYWARQQVQERLAGAELPSDAKPQLGPLSGVIGEIFRYTLESKTLPLYEVKAIQDWVL
                     EREFKKVPGVADVVSWGGGTKQYQVVVDPGRLRAYNLTLKQVFDAVASNNSNAGSGYI
                     AQGDYRIPVRGIGLLQSVADIESIVVAAQKGTPVRVRDIGEVAIGTAIRLGVLGRDHD
                     DDLVQGIVLMRKGENPGQVIEGVKTRAAEVSKLLPAGVELRPYYSRDRLVQTTVHTVL
                     RNLIEGAALVVILLWLFLYNIRAALIVALTIPLSLLFAFIFMDLRGIPANLLSLGAID
                     FGIIVDGAVIMTENIVRRLAERRPRGARVAREVQHAAVEVARPLTFAVLIIMTVYVPI
                     LTFQRIEGKLFRPMAVTISLAVIGALVLTLTLIPVLASLLFRRPPAERESPLLRWLRR
                     PYLPALRWCLTRPRLPVLGAAVGVIAALFVFSLLGKEFLPELDEGDLWVRTQFPIGIS
                     IEAVRPYVREIRERLLKFPEVRVVVSQLGAPDDGTDPEAPDNVEFYVGLRPREEWASP
                     DKEKLVEAMTAALASIPGITTNFSQPIKDNVDEALAGTKGELAIKLYGPDFFVMDAKA
                     KEITAVLRGIRGVADLDYDHAPGQPQLQIVVDRRAAARHGINVQDIQDVIEAATKGRV
                     VTEVFERERRFGIAVRLATPGDPIAALRAVTISAPGGERVPLAQLAEFVRTDGLAEIW
                     REGNVRRIAIKWSVRERDMGGLVVEAMQKVGAAVRLPEGYQMVWSGRFEDQQRALGRL
                     AIIVPLVIFIIFVLLFGAFSSVRDALIIMLNLPFALIGGTLGLFLWGTNFNISAAVGY
                     IAVFGVSVLNGVVLVSSIRQAREDGLPLREAIARGCEIRFRPIVVSAIVAVIGFLPAA
                     LSQGIGAEIQRPLARVVIGGLISSTILTLLVLPAVYALAGRDSERGAALEEDASA"
     gene            complement(3826..>5065)
                     /locus_tag="VNU03_22390"
     CDS             complement(3826..>5065)
                     /locus_tag="VNU03_22390"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWP77290.1"
                     /translation="TEMARYTLATGEPVFTGFMRQRPGPRTWAVVYSLLHLAQVGWAG
                     WALAGGSALAALLLGRMPREEDRAITVGLGYLLFLAAVGLMLAGRHVHRALEILEWIM
                     MAWMLLFLAVLALLYVPRPVWIALGGGFVGRPLWEPGWLPAPPLDRSLDWLLVAGFAA
                     YSGAGGTVNAMLTYWLRDKGFGMAGTVGVQPTSVGAQILLLPREGAIFPPSEANLAKW
                     REWWRYLRADLFSLWTVVCLIGMALPVALALDAVPRHTDVSGLGAGAVFARELARRYG
                     AVLWMPALLTALWIFFSTQVGIAEGFARHVTEMLWTAGARPGAQGPAWLFYPVLALFV
                     AAGGLAMTRAAPLTLILIGANVAALNFAVLSFHTLRLNRALLPEPLRPPRWREAVVAL
                     GGLAFALLVARVLAQVLLGL"
BASE COUNT          742 a         1788 c         1845 g          690 t
ORIGIN      
        1 cggagcgggc cgagcacgcc ctccggctcg cccgcgacct cttcgaggtg cgggcggtgg
       61 cccagaagga ggtgcgggac gcggagagcg agtaccggaa gacggccgcg gagcgggacc
      121 gcgcggccgc acgcctccgc aacctggggg tggatccggc gcggaccgac ggcgccacca
      181 ccgtggcggt gaccgcgccg cgcagcgggg tggtggtcga gcgcaacgtg actccggggc
      241 aggtcgtggc ctacgggcag tccgatctgc cctcgagcct cgtcgtgatc gcggatctct
      301 ccaccatgtg ggtgctcgcc gacgtctacg agcccgacgt cccccgggtg cggctgggcg
      361 aggcggtgtc ggtgacgctg ccctgctgtc cgggcgagcg ctaccagggg caggtggcct
      421 ccatcgggga cgccatcgac aaggagtcgc gcaccctcaa ggtgcgcgcg gtggtgccca
      481 accgcgggcg ggccctcaag agcgagatgt tcgcgcgggt cagcatcgat accgggacgc
      541 gccaggccct cgtggtgccc cagggggcca tccaccgcga cggcagcgcg acgttcgtcc
      601 tggtcgagaa gacgcgggga gaatacgagc gccggccggt caccgtcggg gcggaggccg
      661 acggggtggt cgagatccgc gacggcctga ccccggccga cacggtggtc agcgcgggcg
      721 ggatcctcct gaagcgctcg gcccgatgat cgagcggatc gtcgccttcg cgctgcatca
      781 gcgcttcgtg gtgctggccc tgaccttgat cctcaccgtc ggcggcatcg tctcgtacgg
      841 ccacctgccc atcgaggcct acccggacgt gggcgacgtg aaggtggaga tcatcaccct
      901 ctggcccggg cacgccgccg aggaggtgga gcgcctcatc acgatcccgc tggagaacga
      961 gctgaacggc atcgccaacg tcaccttcct ccgctcggac acgctgttcg ggctctccaa
     1021 catccgggtc ctcttcgcgg acggcaccga cgactactgg gcccgccagc aggtgcagga
     1081 gcggctcgcg ggcgcggagc tcccgtcgga cgccaagccc cagctgggcc cgctctccgg
     1141 agtgatcggg gagatcttcc gctacaccct cgagtcgaag accctgccgc tctacgaggt
     1201 caaggcgatc caggactggg tcctcgagcg cgaattcaag aaggtgccgg gagtggcgga
     1261 tgtggtgtcg tggggcggcg gcaccaagca gtaccaggtg gtggtggatc cggggcggct
     1321 gcgcgcgtac aacctcacac tcaagcaggt cttcgacgcg gtggcgagca acaactccaa
     1381 cgcgggcagc ggctacatcg cccaggggga ctaccgcatc ccggtgcggg gcatcggcct
     1441 cctgcagtcg gtggccgaca tcgagagcat cgtggtggcg gcccagaagg gcacgccggt
     1501 gcgggtgcgc gacatcgggg aggtggccat cggcaccgcg atccgcctgg gcgtgctcgg
     1561 acgcgaccac gacgacgacc tggtgcaggg catcgtgctg atgcgcaagg gcgagaaccc
     1621 gggccaggtc atcgaggggg tgaagacccg cgcggccgag gtgtcgaagc tcctgcccgc
     1681 gggggtcgag ctgcggccgt actacagccg cgaccggctc gtgcagacca ccgtgcacac
     1741 cgtgctgcgc aacctgatcg agggcgccgc cctggtggtc atcctgctct ggctcttcct
     1801 ctacaacatc cgcgccgccc tcatcgtggc cctcaccatt cccctctcgc tgctgttcgc
     1861 gttcatcttc atggacctcc gcgggatccc ggccaacctg ctctccctgg gggcgatcga
     1921 cttcgggatc atcgtcgacg gcgcggtgat catgaccgag aacatcgtgc gccggctggc
     1981 cgagcgccga ccgcgcggcg cccgggtggc gcgggaggtg cagcacgcgg cggtggaggt
     2041 ggctcgcccg ctcaccttcg cggtgctgat catcatgacg gtgtacgtgc cgatcctcac
     2101 cttccagcgg atcgagggca agctgttccg cccgatggcg gtcacgatct ccctcgcggt
     2161 gatcggggcc ctcgtcctca ccctcaccct catcccggtg ctggcgagcc ttctcttccg
     2221 ccgcccgccc gccgagcggg agagcccgct cctccgctgg ctgcgacggc cgtatctccc
     2281 cgcgctccgg tggtgcctca cgcggccgcg gctgccggtg ctgggggcgg cggtcggggt
     2341 gatcgccgcc ctcttcgtct tctcgctgct cggcaaggag ttcctgcccg agctggacga
     2401 gggcgacctg tgggtgcgca cccagttccc catcggcatc tcgatcgagg cggtgcgccc
     2461 ttacgtgcgc gagatccgcg agcggctgct caagttcccc gaggtgcggg tggtcgtctc
     2521 ccagcttggc gcgccggacg acgggaccga cccggaggcg cccgacaacg tcgagttcta
     2581 cgtcgggctc cggccccgcg aggagtgggc gagcccggac aaggagaagc tggtggaggc
     2641 gatgaccgcg gccctcgcga gcatccccgg gatcaccacc aacttctcgc agccgatcaa
     2701 ggacaacgtg gacgaggcgc tcgcggggac caagggcgag ctggccatca agctctacgg
     2761 acccgacttc ttcgtgatgg acgccaaggc caaggaaatc accgcggtcc tgcggggaat
     2821 ccgcggggtc gccgacctcg actacgatca cgcccccggc cagccgcagc tgcagatcgt
     2881 ggtcgaccgg cgcgcggccg cccgccacgg gatcaacgtg caggacatcc aggacgtcat
     2941 cgaggccgcc accaagggtc gggtcgtgac cgaggtcttc gagcgggagc gccgtttcgg
     3001 catcgcggtg cggctcgcca cccccggcga tccgatcgcg gccctgcgcg cggtgaccat
     3061 cagcgcgccc ggcggggagc gggtgccgct cgcccagctc gccgagttcg tccgcaccga
     3121 cggcctcgcc gagatctggc gcgagggcaa cgtccggcgc atcgcgatca agtggagcgt
     3181 ccgcgagcgg gacatgggcg gcctggtggt ggaggccatg cagaaggtgg gcgccgcggt
     3241 gcggctgccc gagggctacc agatggtctg gagcggtcgc ttcgaggacc agcagcgggc
     3301 gctggggcgg ctcgcgatca tcgtgccgct cgtgatcttc atcatcttcg tgctgctgtt
     3361 cggcgcgttc agctcggtgc gcgacgcgct catcatcatg ctcaacctgc cgtttgccct
     3421 gatcggcggc accctcgggc tcttcctgtg gggcacgaac ttcaacatct cggccgcggt
     3481 gggctacatc gcggtcttcg gggtcagcgt cctcaacggg gtggtgctcg tctcctccat
     3541 ccggcaggcg cgggaggacg ggctgcccct gcgcgaggcc atcgcgcgcg gctgcgagat
     3601 ccgcttccgc ccgatcgtgg tgtccgcgat cgtggcggtg atcggcttcc tgcccgcggc
     3661 cctgtcccag ggcatcggcg cggagatcca gcggccgctc gcccgggtgg tcatcggggg
     3721 gctgatctcc tcgacgatcc tgaccctgct ggtcctcccc gcggtgtacg cgctcgccgg
     3781 gcgcgactcc gagcgcggcg cggcgctcga ggaggacgcc tccgcctaga gcccgagcag
     3841 gacctgcgcg aggacgcgcg cgaccagcag cgcgaaggcg agcccgccca gggccaccac
     3901 cgcctcccgc cagcgcggcg gccgcagcgg ctccgggagc agcgcgcggt tcaggcgcag
     3961 ggtgtggaag gacaggaccg cgaagttgag cgcggccacg ttggccccga tgaggatcag
     4021 ggtgagcggc gccgccctcg tcatggcgag gccgcccgcc gccacgaaga gcgcgagcac
     4081 cgggtagaag agccaggccg gtccctgggc tccgggccgc gcgccggcgg tccacagcat
     4141 ctcggtcacg tggcgggcga agccctccgc gatccccacc tgggtcgaga agaagatcca
     4201 gagcgcggtg agcagcgcgg gcatccagag caccgccccg tagcgccgcg ccagctcgcg
     4261 cgcgaacacc gcgcccgcgc cgaggccgct gacgtcggtg tgtcgtggca ccgcgtcgag
     4321 ggccagcgcc accggcaggg ccatgccgat caggcacacc accgtccaga gcgagaagag
     4381 atcggcgcgg agatagcgcc accactcgcg ccacttggcc aggttggcct cgctcggcgg
     4441 gaagatcgcg ccctcgcgcg ggagcaggag gatctgggcg cccaccgagg tcggctggac
     4501 gcccaccgtc cccgccatgc cgaagccctt gtcgcgcagc cagtaggtga gcatcgcgtt
     4561 gacggtgccg cccgcgcccg agtaggccgc gaagcccgcg acgagcagcc agtcgagcga
     4621 gcgatcgagc ggcggcgcgg gcagccagcc gggctcccag aggggccgac ccacgaagcc
     4681 cccgccgagc gcgatccaca ccggccgcgg cacgtagagc agcgcgagga cggccaggaa
     4741 cagcagcatc caggccatca tgatccactc gaggatctcg agcgcgcgat ggacgtggcg
     4801 accggcgagc atcaggccca ccgcggcgag gaagagcagg tagccgagcc ccaccgtgat
     4861 cgcgcggtcc tcctcgcgcg gcatgcgccc gaggagcagc gcggcgagcg cggagccgcc
     4921 cgcgagggcc cagcccgccc agccgacctg ggccaggtgc agcagcgagt agacgacggc
     4981 ccaggtgcgc gggccggggc gctggcgcat gaagccggtg aacaccggct cgccggtcgc
     5041 gagggtgtag cgggccatct cggtg
//
LOCUS       DATMCV010000981         3156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_588911, whole genome shotgun sequence.
ACCESSION   DATMCV010000981 DATMCV010000000
VERSION     DATMCV010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588911"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VNU03_22395"
     CDS             complement(<1..519)
                     /locus_tag="VNU03_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77291.1"
                     /translation="MVLAAAVVIIHGPFGDLRWWRNVAVLGLLLVVFESTATLLRPRQ
                     LAWSPNSSATLAAAVLLGPVGAGLVGACTIFGMRRGPSVAQRAFNTGMMALSAYLAGR
                     VFVAVGGQVGTPHSTPFRTIIGPFAVAAVVHAVTNHTLLAGVLWLTSDRDFAGRRAPL
                     GMSVKLIASDLGY"
     gene            complement(905..1177)
                     /gene="rsrA"
                     /locus_tag="VNU03_22400"
     CDS             complement(905..1177)
                     /gene="rsrA"
                     /locus_tag="VNU03_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HWP77292.1"
                     /translation="MSCGKPHETPCTEVLERVYGYLDGELGDVDCAKIRQHLDECGPC
                     LREYGLEEAVKRLVAKHCGCDPAPADLRAKILVRIREVRATIEFAE"
     gene            complement(1174..1791)
                     /locus_tag="VNU03_22405"
     CDS             complement(1174..1791)
                     /locus_tag="VNU03_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP77293.1"
                     /translation="MSPVPETLEERGERFEQEVLPFLDQLYSAALRMTRNPADAEDLV
                     QETFAKAYASFHQFQQGTNLKAWLFRILTNTFINTYRKRQREPQRSGAEEVEDWQLAR
                     AAEHTSGGLKSAEAEALERLPDSDIKEALQALPEDFRIAVYLADVEGFAYKEIADIME
                     TPIGTVMSRLHRGRRQLRELLQDYASERGLARTGSDASRPGEEAR"
     gene            2119..2775
                     /locus_tag="VNU03_22410"
     CDS             2119..2775
                     /locus_tag="VNU03_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWP77294.1"
                     /translation="MAQAQLLAWLPRGQHMIVDVSGPRSPGGELAAMAGHHVLRVLER
                     DNRASGTKTARLIGDSSRLSFLPDECADDVLAEDRALSTHLGAEAMIAEIARVLRPAG
                     RVLACVDSLVLGMAVLADQNHWAHLVDLPYAEVVLVPWPDGTITRCYGPDQARELFTG
                     AGLTVNWIRPRTVFSESMVTHVLRRDPGSLAKLVRAELAARSDESLGAQLVISAVKKG
                     "
     gene            complement(2772..>3156)
                     /locus_tag="VNU03_22415"
     CDS             complement(2772..>3156)
                     /locus_tag="VNU03_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HWP77295.1"
                     /translation="GAAAPADGRRGPMEITVARKRLEEMRDDLDRSISILQGERPAPS
                     AGSGYPQDSADAGSSLSEADRTEAILHSARGQRDEVLAALVRIEEHSYGRCVDCAGDI
                     PEGRLDARPDAARCVGCQAKRARRR"
BASE COUNT          522 a         1102 c         1064 g          468 t
ORIGIN      
        1 gtagccgagg tccgaggcga tcagcttgac gctcatgccc agcggagcgc gccgcccggc
       61 gaagtcgcgg tcgctggtca gccagagcac ccccgcgagc agggtgtggt tggtcaccgc
      121 gtgcaccacg gcggcgaccg cgaacgggcc gatgatggtc cggaacggcg tggagtgcgg
      181 ggtgccgacc tgcccgccca cggcgacgaa gacgcggccc gccaggtacg cggacagggc
      241 catcatgccc gtgttgaacg cccgctgggc caccgaggga ccgcgccgca tcccgaagat
      301 ggtgcaggcg ccgaccagcc ccgcgccgac cggcccgagc aagacggcgg cggccagggt
      361 ggcggaggaa ttgggggacc aggcgagctg ccgggggcgc agcaaggtgg cggtcgactc
      421 gaagaccacg agcagcaggc cgagcaccgc gacattccgc caccagcgga ggtcgccgaa
      481 cggaccatga atgatcacaa ccgcggcggc gaggaccacc acgccgatgt atagccgtgc
      541 ggcccacgag agcctagcca aaccctcctc ccctcaccgc caggggcgcg gaacgcgggc
      601 tatgctccaa ccccttaccc ccacgtgctc ccggaggggg cggtcagaca gaccgggccc
      661 gccgtcgcct cacccgcagc acgcgatatt ccgccgcatg gacgcatgga gctgacctcc
      721 ctaacgtgca actaactaca gctcaccgga tatcgtccaa ggtcaaggat aacgtggcca
      781 caacggatct cgcaggccca aatctcatcg tgaacaagtc gtgagcgtga tagtcacaat
      841 gggcaatcaa atacggcaca tgctgcgcaa ttgccccact cctggcaaat cggacggttc
      901 gcgactactc cgcgaactcg atcgtcgccc ggacttcacg gatacggacg agaattttcg
      961 cgcgcaggtc ggcgggcgcc gggtcgcagc cgcagtgctt ggccacgagc cgcttaaccg
     1021 cctcctcgag gccgtactcg cgcaggcagg ggccgcattc gtccagatgc tggcggatct
     1081 tcgcgcagtc cacgtcgccc agctcgccgt ctagatagcc gtagacccgc tccagtacct
     1141 cggtacacgg cgtctcatgt ggctttccgc agctcatcgc gcttcctccc cgggccggga
     1201 agcgtcagaa ccggtgcgcg ccaatccgcg ttccgacgcg taatcctgga gcagttcccg
     1261 cagctgccgc cgaccgcggt gcagacgcga catcacggtc ccaatcggcg tttccatgat
     1321 gtcggcgatc tccttgtacg cgaacccctc gacatcggcc aggtagaccg cgatgcggaa
     1381 atcctccggg agcgcctgga gggcttcctt aatgtcagaa tcgggcagcc gttccagcgc
     1441 ttcggcttcg gccgacttca atccccccga ggtatgctcg gccgcccggg ccagctgcca
     1501 gtcctcgacc tcctccgcgc ctgagcgctg cggctcgcgt tgccgcttgc gataggtgtt
     1561 gataaaggtg ttggtcagga tccggaacag ccacgccttg aggttcgtgc cctgctggaa
     1621 ctggtggaaa gacgcgtacg ccttggcgaa cgtctcctgt accaggtcct cggcgtccgc
     1681 cgggttgcga gtcatccgaa gcgccgcgga gtacagctga tccaggaagg gcagcacctc
     1741 ctgctcgaag cgctcaccgc gctcttcgag agtctccgga acgggactca ccccctcggg
     1801 gtcggacgga ctaggtctgc ttgggtgcag agcatatgag ggggaggata ccgcgcccgg
     1861 atacggagcg agtctcagca tgccacgagg tggcgaggcc acagccatgt cgcgttcaac
     1921 acgagggcgc cgcccgtcat tccgagatcg gtccgggcag gcaggtgaga aaggcgtctg
     1981 gccgggaagg ttaaggacaa agggcatccc cgctgcggga atgccgagtc aggagccggg
     2041 cccgtgcacc atgcggccac cgccatcacc gcgccggata ctgatcatct tccggcctac
     2101 tggcaattcc acgacgccgt ggcgcaggct cagctgctgg cctggctgcc gcgcggacag
     2161 cacatgatcg tggacgtctc gggaccgcgc tcccccggcg gcgaactcgc cgccatggcc
     2221 ggccaccacg tgctgcgagt ccttgagcgg gacaacagag cgagcgggac gaagacagct
     2281 cgcctgatcg gggactcctc gcggctgtcc ttcctgcccg acgagtgcgc ggacgacgtg
     2341 ctggccgagg accgcgcgct gtccacccac ctgggcgccg aggccatgat cgccgagatc
     2401 gcccgcgtgc tgcgtccggc cgggcgggtg ctggcctgcg tcgactcgct cgtgctgggc
     2461 atggcggtgc tcgccgacca gaaccactgg gcgcacctgg tcgacctgcc gtacgccgag
     2521 gtggtactgg tgccctggcc ggacggcacc atcacccggt gctacggccc cgaccaggcg
     2581 cgcgagctgt tcaccggggc gggcctgacg gtgaactgga tccggccgcg caccgtgttc
     2641 tccgagtcga tggtcacgca cgtgctgcgc cgcgacccgg gcagcctggc caagctggtc
     2701 cgcgccgaac tggccgcgcg atccgatgag tcgctgggcg cgcagctggt gatctcggcg
     2761 gtcaagaagg gctagcggcg gcgggcgcgc ttggcctggc agccgacgca gcgggccgcg
     2821 tcgggccgcg cgtcgagccg gccctcggga atgtcgcccg cgcagtcgac gcatcgcccg
     2881 tagctgtgct cctcgatgcg cacgagcgcg gcgagcacct cgtctcgctg gccgcgggcc
     2941 gagtgcagga tcgcttccgt acgatcggcc tcggacaagc tggaacccgc gtcggccgag
     3001 tcctgcggat accccgaccc ggccgagggc gcgggacgct cgccctgcag gatggatatc
     3061 gaccggtcca ggtcgtcgcg catctcctca aggcgcttac gggcgacggt aatctccatt
     3121 ggtcctcgcc tcccgtccgc gggtgctgcg gcgccc
//
LOCUS       DATMCV010000982         7223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_924913, whole genome shotgun sequence.
ACCESSION   DATMCV010000982 DATMCV010000000
VERSION     DATMCV010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924913"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VNU03_22420"
     CDS             complement(<1..798)
                     /locus_tag="VNU03_22420"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWP77296.1"
                     /translation="MRIAVLGAGGIGGYYGARLAKAGHDVVLVARGAHLEALQRRGVT
                     VRTPDGELTLPITAVPDVRGLEPVDLLLFCVKAYDTETGAHSIAPLVGPDTVVLTVQN
                     GLDRVEALATALGGATAVLAGAVYAALQLADPGVVVRTGPEARITFGERGGAASDRTA
                     RLAAALRESGLVHEVSPDIDRVLWEKFLFITGIGAVTALARAGIGALRERGEGRALLA
                     ASCGEIVAVAQAEGAPLRPEAAAAALAQASTLPAGWRSSMARDLEDGR"
     gene            903..1388
                     /locus_tag="VNU03_22425"
     CDS             903..1388
                     /locus_tag="VNU03_22425"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWP77297.1"
                     /translation="MTRGAAPLSPCVCNTLRMATRVVTQLYDDVLRPSGLRVTQFSML
                     AAIARLGLANLRQLEEALAIDQTTLTRSLGLLERDGLTERVAHPDRRIKSMRLTARGR
                     AVLRTARPLWSRAQATVLRELGSTGWADARRRLAHLRDVAVRTRRSRRARGRRTASRP
                     A"
     gene            complement(1330..2334)
                     /locus_tag="VNU03_22430"
     CDS             complement(1330..2334)
                     /locus_tag="VNU03_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77298.1"
                     /translation="MSISLRAPIGILDEHPEWSSRLTAELRARRLPHERIDHSNHAYD
                     PRDREPRYSVIVNRTSPSSATRGHGSVLFYAEALLGHYASLGVPIINPVAAYRSEKSK
                     ALQLDLLERVGARYPRTVVVNHRDQLWKALDEIPFPLVVKPNIGGSGAGIVRFDSHEA
                     LEAGVEGLDFGPDGTALVQEYLESDEGAIVRVEVLDGRYLYAIRIVRDAQAGFNLCPA
                     DICQVPGEAAASATPLEACPAGPKPGLTVTRFDAPPEAIETVLRLTRAAAIDIGGVEY
                     LVARRDGQIYYYDINATSNFVANAPALLGFDPTARFVDYITRVATRSAAPAPVAIAAS
                     "
     gene            2497..4113
                     /locus_tag="VNU03_22435"
     CDS             2497..4113
                     /locus_tag="VNU03_22435"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77299.1"
                     /translation="MLTRRDFLAAGTAAAALATYPSRALAQSPKRGGTLAIRAWDPPH
                     FDASLVHAYKTHVAISFTHSRLLRYRAGAGVKPGSLVIEGDLAESWTQPSDTTYVFKL
                     RRGVRFHAKPPVNGRELTADDVKFTFERMLTEAGSNASMYRAIAKVEAVDRYTVRFTL
                     SEPFAWFLDMVASPMAGGIVARECVEKWGDLRKPEAVIGTGPWMLESYRPNVSLTMVR
                     NPGYFVPGLPYIDRVELMIDEDNASRMAAFLAGRYDLGWEFAGTINRTDWVQIKDRLR
                     QTRPTLRTVEFPSNVVNDLAMRTDQKPWSDVRVRQAVSMAIDRREIIEATLEGVGVVN
                     GPLPAALGDWALPIDQLGEGARNYRHDPAEAKRLLAAAGYPSGFPASVCFNSYGSTVL
                     VDTMQLVLKHLKAVGIDARLDQKEYGAYQATCRLGKFDSTVFGPLTPFLDADNFLFGQ
                     YFTGEPRNRSHVKDPALDDLLVRQRRTMDVKARRDVINDIQRHLARQQYYVHVPTGTY
                     IAVWDGALKNYGPNLGYDYGGRLLTAWLDR"
     gene            4121..4450
                     /locus_tag="VNU03_22440"
     CDS             4121..4450
                     /locus_tag="VNU03_22440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP77300.1"
                     /translation="MTERDRIAEFKEVVEMMPDDPVVRFGLAGAYLDAGQADLAAAEY
                     AEAVRLKPDYSAAHRGLGRALERAGRPADAAAAYTIGLEVASRTGDLQTKKEIEVFLR
                     RLTQAGT"
     gene            4551..4904
                     /locus_tag="VNU03_22445"
     CDS             4551..4904
                     /locus_tag="VNU03_22445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77301.1"
                     /translation="MRQPSRLAVAGIAIALLAAAWPGAAAAESTSQDVAYGVGSVATT
                     LVYAPIKASFCLLGAVTSGFTLPFGGPRTAEKVASTACGGTWMITPAALKGREPIHFV
                     GGGAGEPSGRAAARR"
     gene            4918..5697
                     /locus_tag="VNU03_22450"
     CDS             4918..5697
                     /locus_tag="VNU03_22450"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWP77302.1"
                     /translation="MVLADRVAVVTGAGSGIGKATALALARAGARVAVVDVDGAAAKV
                     TADAIGALGSRGLALETDVGDLAAIDRMVQAVTGALGGIDVLVNNAGVTRRAYIMDLT
                     EQDWDRIMRVNAKGVFFCLQRVAREMIPRRRGRIVNIASIAGKGYAGTSNAIYAASKG
                     SVISLTRTAAQQLARHDINVNAICPGITVTALSEANVAVRARDEGLPLEEMTRRRNAV
                     IPLGRPNDPEDVAALAVFLASPAARNITGQSLNVDGGIIFD"
     gene            5720..6568
                     /locus_tag="VNU03_22455"
     CDS             5720..6568
                     /locus_tag="VNU03_22455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP77303.1"
                     /translation="MNVGISVPLPAYLVDVGAMARTAEALGFESFWCAEHPFIPVHTT
                     SRFPGTADGVIPEAYSHFIDPFVALARASGTTSRIKLATGIVLVPERHPLMLAKEVST
                     LDLFSGGRFLFGIGAGWLREETQLMGGDFDHRWGQTRESILAMKELWTKPEAEFHGKF
                     YDFPPVRSNPKPAQKPHPPVLLGGDAPRVLERVVAWGDGWLPNRVTPVELREKRATLD
                     RLAKEAGRDPAAISISVHGQPADRDLIARLHEAGATRVIVRPATSKSEPEMVRELTRI
                     AEAVLR"
     gene            complement(6581..>7223)
                     /locus_tag="VNU03_22460"
     CDS             complement(6581..>7223)
                     /locus_tag="VNU03_22460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77304.1"
                     /translation="QSLAAIVADAFACLKWLAVDPLDFDSVREALIAVVKDGERAADV
                     IGRIRALLSRSRVAHEPCDLAAIVQDVLPLVAPEMSRHGISLETGLAPELPRVLGDRI
                     QLQQVLLNLLVNAAEAMRETPPERRRVVVRSTTESRDDGAWAVLTVEDAGVGFREQDE
                     TRLFEAFYTTKPSGLGMGLSISRSIIERHGGRLWATPNPDHGATFHFALPAIR"
BASE COUNT         1050 a         2595 c         2593 g          985 t
ORIGIN      
        1 ccggccgtcc tcgaggtcgc gggccatcga cgagcgccag ccggcgggca gggtggaggc
       61 ctgggcgagg gccgccgcag cagcctcggg gcgcaggggg gccccttccg cctgggccac
      121 cgcgacgatc tcgccgcagg aggcggcgag cagcgcacgg ccctcgcccc tctcccggag
      181 cgcgccgatg ccggcgcggg cgagcgcggt caccgccccg atcccggtga tgaacaggaa
      241 cttctcccac agcacccggt cgatgtcggg cgagacctcg tggacgaggc cgctctcgcg
      301 gagggcggcg gcgaggcgcg cggtccggtc gctcgcggcc ccgccccgct cgccgaacgt
      361 gatccgggcc tcgggaccgg tgcggacgac gacgcccggg tcggcgagct ggagcgcggc
      421 gtacaccgcg cccgcgagca cggccgtcgc tccgccgagt gcggtcgcga gcgcctcaac
      481 acggtccaga ccgttctgga cggtaaggac gacggtgtcg ggcccgacca ggggggcgat
      541 cgagtgggcg ccggtctcgg tgtcgtaggc cttgacgcag aacagcagga ggtcgacggg
      601 ctcgaggccc cgcacgtcgg gcaccgcggt gatgggcagg gtcagctccc cgtccggggt
      661 ccggaccgtc accccgcgtc gctgcagggc ctccaggtgt gcgccccggg cgacgagcac
      721 cacgtcgtgg ccggccttgg ccagccgcgc cccgtagtag cctccgatgc cgcccgcgcc
      781 caggaccgcg atccgcatgg gtgagcctcc tggcgattga gacgcccggg cgcggccgcg
      841 ggattcatgt atctacatgg tcaccgcttg cccgggcgga gggcggctgc tagcatggga
      901 ccatgacgcg cggggcggcg ccgctctctc cctgtgtctg caacaccctc cggatggcga
      961 cccgggtggt gacgcagctc tacgacgacg tgctgcggcc gagcggcctg cgggtgaccc
     1021 aattcagcat gctcgcggcg atcgcgcgcc tcggcctcgc gaacctccgg cagctcgagg
     1081 aggcgctcgc gatcgaccag accacgctca cccgcagcct gggcctactc gagcgcgacg
     1141 gcctgaccga gcgcgtggcc caccccgacc ggcggatcaa gtcgatgcgc ctcaccgcca
     1201 ggggcagggc ggtcctccgg acggcccggc cgctctggag ccgggctcag gcgacggtgc
     1261 tgcgggagct ggggagcacg gggtgggcgg acgcgcgccg gcgtctcgcc cacctgcgcg
     1321 acgtcgcggt caggacgcgg cgatcgcgac gggcgcgggg gcggcggacc gcgtcgcgac
     1381 ccgcgtgatg tagtcgacga agcgcgcggt cggatcgaaa ccgagcagcg cgggcgcgtt
     1441 ggccacgaag ttcgaggtgg cgttgatgtc gtagtagtag atctgcccgt cccgccgggc
     1501 caccaggtat tccaccccgc cgatgtcgat ggcggccgcg cgggtgaggc gcagcacggt
     1561 ctcgatcgcc tcgggcggcg cgtcgaaccg ggtgacggtg aggccgggct tgggcccggc
     1621 ggggcaggcc tcgagcggag tggcggaggc ggcggcctcg cccggtacct ggcagatgtc
     1681 ggccgggcac aggttgaagc cggcctgcgc gtcgcgcacg atccggatcg cgtagaggta
     1741 gcggccgtcc aggacctcca cccgcacgat cgcgccctcg tcggactcga ggtactcctg
     1801 caccagagca gtcccgtccg gcccgaagtc gagcccctcg actcccgcct cgagggcctc
     1861 atgcgagtcg aagcggacga tccccgcccc ggagccgccg atgttcggct tgaccacgag
     1921 cgggaacggg atctcgtcga gcgccttcca gagctgatcg cgatggttga cgacgaccgt
     1981 tcgcggatag cgcgcgccga cgcgctcgag cagatcgagc tggagcgcct tcgatttctc
     2041 gctgcggtac gcggccaccg gattgatgat cggcaccccg agcgaggcgt agtggccgag
     2101 cagggcctcc gcgtagaaca acacgcttcc gtggccgcgg gtggccgagg acgggctggt
     2161 ccggttcacg atcaccgagt agcggggctc gcggtcccgc gggtcgtacg cgtggttgga
     2221 gtggtcgatg cgctcgtggg gcagccggcg agcccggagc tcggccgtga gccggctcga
     2281 ccactccggg tgctcgtcca ggatcccgat cggggcccgc agggatatgc tcatgtccgc
     2341 ctctttccag agatttcgat gttgtaccta ttccctaccg acaaccgtga taatcgacca
     2401 gatttccagg cctgtcaagc ccgcccgagg tccggtattc tctggcccac cgagcgcctc
     2461 gatcgtcgac ggccggcgcc ggagggaggc acgtcaatgc tgactcgccg ggactttctc
     2521 gccgccggga ccgcagccgc ggcgctcgcc acctatccgt cgcgtgcgct cgcccagtct
     2581 cccaagcgcg ggggcaccct cgcgatccgg gcctgggacc cgccccactt cgacgcgagc
     2641 ctcgtacacg cctacaagac tcacgtggcg atctcgttca cccacagccg gctgctccgc
     2701 tatcgggccg gggcgggggt caagccgggc tcgctggtga tcgagggcga cctggccgag
     2761 tcctggacgc agccgagcga caccacctac gtcttcaagc tgcgacgcgg ggtacgcttc
     2821 cacgccaagc cgccggtcaa cggccgtgag ctgaccgcgg acgacgtgaa gttcaccttc
     2881 gagcgcatgc tgaccgaggc gggcagcaac gcgtcgatgt accgcgcgat cgcgaaggtc
     2941 gaggcggtcg accgctacac ggtgcgcttc acgctctccg agcccttcgc ctggttcctc
     3001 gacatggtgg cgagccccat ggcgggcggc atcgtggcgc gcgagtgcgt ggagaagtgg
     3061 ggcgacctca ggaagccgga ggcggtgatc ggcaccgggc cgtggatgct cgagagctac
     3121 cggccgaacg tgtcgctcac catggtccgc aatcccggct acttcgtgcc cgggctgccg
     3181 tacatcgacc gggtcgagct gatgatcgac gaggacaacg cctcgcgcat ggccgccttc
     3241 ctggccgggc gctacgacct gggctgggag ttcgcgggga cgatcaaccg gacggactgg
     3301 gtgcagatca aggaccggct gcgccagacg cggccgaccc tgcgcaccgt cgagttcccc
     3361 tccaacgtgg tgaacgatct cgcgatgcgc acggaccaga agccgtggag cgacgtgcgg
     3421 gtgcgccagg cggtctcgat ggcgatcgac cggcgcgaga tcatcgaggc gacgctcgag
     3481 ggcgtgggcg tggtcaacgg gccgctgccc gctgcgctcg gcgactgggc cctgcccatc
     3541 gaccagctcg gcgagggtgc ccgcaactac cgccacgatc ccgccgaggc caagcgcctg
     3601 ctcgccgccg cgggctaccc gtcgggcttc cccgccagcg tgtgcttcaa ctcctacggc
     3661 tcgaccgtgc tggtcgacac catgcagctc gtgctcaagc acctgaaggc ggtgggcatc
     3721 gacgcgcggc tcgaccagaa ggagtacggg gcctaccagg cgacgtgccg gctcggcaag
     3781 ttcgactcca ccgtgttcgg gcccctgacc ccgttcctcg acgcggacaa cttcctcttc
     3841 ggccagtact tcaccgggga gccgcgcaac cggagccacg tgaaggatcc cgcgctcgac
     3901 gatctcctgg tccggcagcg ccgcaccatg gacgtgaagg cgcgccggga cgtcatcaac
     3961 gacatccagc gccacctggc caggcagcag tactacgtgc acgtcccgac cgggacctac
     4021 atcgcggtgt gggacggcgc gctcaagaac tacggcccga atctgggcta cgactacggc
     4081 gggcggctcc tcaccgcctg gctcgaccgc tgagccccgc gtgaccgagc gggatcggat
     4141 cgcggagttc aaggaagtgg tcgagatgat gcccgacgac ccggtggtcc ggttcggcct
     4201 cgccggcgcc tatctcgacg cggggcaggc cgacctggcc gccgcggagt acgcggaggc
     4261 ggtccggctc aagcccgact actcggccgc ccatcgcggc ctcggccgcg ccctcgagcg
     4321 ggcgggccgt ccggccgacg cggccgccgc gtacacgatc ggcctggagg tggcgtcgcg
     4381 caccggggac cttcaaacca agaaggagat cgaggtcttc ctccggcggc tgacccaggc
     4441 cgggacgtga cgtcgagcgg aggccgcgcg ctttacagcg cccccggcgg cgggataggc
     4501 tgggcgggat tcgccgagaa tccatagcca gatccaggag gtcgccgcgc atgagacagc
     4561 cgtcgagact cgccgtcgca gggatcgcga tcgccctgct cgccgccgcg tggcccgggg
     4621 ccgccgccgc ggagagcacg tcgcaggacg tcgcctatgg ggtgggcagc gtcgccacca
     4681 cgctcgtgta cgcgccgatc aaggccagct tctgcctcct gggagcggtg acgagcgggt
     4741 tcacgctgcc gttcggcggg ccccgcaccg cggagaaggt cgcgtcgacc gcgtgcggcg
     4801 gcacgtggat gatcacgccc gccgcgctca agggccggga gccgattcac ttcgtgggcg
     4861 gcggcgcggg cgagccgtcc ggccgggccg cggcccgacg gtagccggcg gcccggcatg
     4921 gttctcgcgg atcgcgtcgc ggtcgtcacc ggggccggca gcgggatcgg caaggcgacc
     4981 gcgctggccc tggcccgcgc gggcgcgcga gtcgcggtgg tcgacgtcga cggcgcggcg
     5041 gccaaggtca ccgcggacgc gatcggcgcg ctcggatccc gcggcctggc cctcgagacc
     5101 gacgtgggcg acctggccgc gatcgatcgc atggtgcagg cggtgaccgg cgcgctcggc
     5161 gggatcgacg tcctggtgaa caacgcgggg gtcacgcgcc gcgcctacat catggatctc
     5221 accgagcagg actgggaccg catcatgcgg gtcaacgcca agggcgtctt cttctgcctg
     5281 cagcgggtgg cgcgggagat gatcccgcga cggcgcggcc gcatcgtcaa catcgcgtcg
     5341 atcgcgggca aggggtacgc gggcacctcg aacgcgatct acgcggccag caaggggagc
     5401 gtcatctcgc tcacccgcac cgcggcccag cagctcgcgc ggcacgacat caacgtcaac
     5461 gcgatctgcc cgggcatcac ggtcaccgcg ctctcggagg ccaacgtggc ggtgcgcgcg
     5521 cgcgacgagg gcctgccgct cgaggagatg acgcgccggc gcaacgcggt catcccgctc
     5581 ggccgcccca acgacccgga ggacgtggcc gccctcgcgg tgttcctcgc ctcgccggcc
     5641 gcgcggaaca tcaccggcca gtcgctcaac gtcgacggcg gcatcatctt cgactgaccg
     5701 cccaccccag gaggctccca tgaacgtcgg catctccgtc ccgctgcccg cctatctcgt
     5761 cgacgtgggg gccatggcgc gcaccgccga ggcgctcggc ttcgagtcgt tctggtgcgc
     5821 cgagcatccg ttcatcccgg tgcacaccac cagccgcttc cccggcaccg cggacggcgt
     5881 catccccgag gcctactcgc acttcatcga tccgttcgtg gccctggccc gtgcctccgg
     5941 gacgaccagc cggatcaagc tcgcgaccgg aatcgtgctg gtcccggagc gccacccgct
     6001 gatgctcgcc aaggaggtgt ccacgctcga cctgttcagc ggcgggcgct ttctcttcgg
     6061 catcggggcc ggctggctgc gggaggagac ccagctcatg ggcggcgact tcgaccaccg
     6121 ctggggccag acccgcgagt cgatcctggc catgaaggag ctgtggacga agccggaggc
     6181 cgagttccac ggcaagttct acgacttccc gccggtccgc tccaatccca agccggccca
     6241 gaagccgcac ccgccggtgc tcctgggcgg cgatgccccg cgggtgctcg agcgcgtggt
     6301 ggcctggggc gacgggtggc tcccgaaccg ggtcaccccc gtggagctcc gcgagaagcg
     6361 cgccaccctc gaccggctcg ccaaggaggc cggacgcgat ccggccgcca tcagcatctc
     6421 ggtgcacggt cagccggcgg accgggatct gatcgcgcgg ctccacgagg cgggggccac
     6481 gcgggtgatc gtgcgcccgg ccacctcgaa gtcggagccc gagatggtcc gcgagctcac
     6541 ccgcatcgcg gaggcggtgc tgcgctagcg cctccaagcg tcatcgtatc gccggcaggg
     6601 cgaagtgaaa ggtcgccccg tggtccggat tcggggtggc ccacaggcgt ccgccgtgcc
     6661 gctcgatgat cgagcggctg atcgagaggc ccatgccgag cccgctcggc ttcgtcgtgt
     6721 agaacgcctc gaacagacgg gtctcgtcct gctcacggaa gccgaccccc gcgtcctcca
     6781 ccgtcaggac cgcccacgcc ccgtcgtcgc gagactccgt ggtcgatcgc accacgacgc
     6841 ggcgccgctc cggcggagtc tcccgcatcg cctcggccgc gttcacgagc aggttcagta
     6901 gaacttgctg gagctggatc cgatctccga ggacccgcgg cagctccgga gcgagcccgg
     6961 tctcgagcga gatcccgtgg cggctcatct cgggggcgac caggggcagc acgtcctgga
     7021 cgatcgcggc caggtcgcag ggctcgtggg ccactcgcga ccgcgagagc agcgcccgga
     7081 tccggccgat aacgtccgcc gcccgctcac cgtccttgac gaccgcgatg agcgcctccc
     7141 gaacgctatc gaaatcgagg gggtcgacgg ccagccactt caggcacgcg aaagcgtcgg
     7201 cgacgatcgc ggccaaggat tga
//
LOCUS       DATMCV010000983         4511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_120684, whole genome shotgun sequence.
ACCESSION   DATMCV010000983 DATMCV010000000
VERSION     DATMCV010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120684"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VNU03_22465"
     CDS             complement(<1..299)
                     /locus_tag="VNU03_22465"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HWP77305.1"
                     /translation="MSNDPVRARSQFFGGRVCLDFANTVDWRTSASPQELIPDYATLL
                     AWSRMRGTLPAAAVARLRARGVKRAAAAAAVLDDAHALRIEIWQAAIALSGGRPAP"
     gene            333..1037
                     /locus_tag="VNU03_22470"
     CDS             333..1037
                     /locus_tag="VNU03_22470"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HWP77306.1"
                     /translation="MREPGAARPPFTASAFRRGFVAALPFVVSNGVGGVVMGVAYRGL
                     GLDFSSAVLWSLVVYSGTAQAVTLGLWTTSPPIAAMVVACVATNARYLVLGAHLRQLF
                     GDVPRRVMLPILALLADASWLMTVAEAERGRRDAGYLLGSGVPMAIGWIGGTALGHAL
                     PLAPRGPLAVAAAFLPLAFVVVLLPTQWSGARSLLPWTVSAAVALAVAGTLGPSWAML
                     IGGGAGTLVSALRGDA"
     gene            1030..1329
                     /locus_tag="VNU03_22475"
     CDS             1030..1329
                     /locus_tag="VNU03_22475"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HWP77307.1"
                     /translation="MPDSRVLAAIVAVGLASYAMRAGGFLAAGAIPPGGLLERLLRLA
                     PGNLFVAFAAAGMLEGGWPSLVGCLGAVATMAATDREWAALGAGFAAAALASTVL"
     gene            1432..2133
                     /locus_tag="VNU03_22480"
     CDS             1432..2133
                     /locus_tag="VNU03_22480"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP77308.1"
                     /translation="MGRLARRLGLITIVAVACAAPVPPASAADPVVLASGALQRCLGI
                     TPGQAKFNGMSAAGAPYCEQLPGASAYAQAGQRYQAGDRAGAVKLVTSAAQAGHPLAQ
                     LRLAIMYEGADGVPRDKKAAFTWYARAAAVGEPAAQRELAGYYEDGDGVAENWDLAAK
                     LYQASATQGWSKGQLALGRAYEFGIGVPQDRQQAVAWFARAGPRDSARASTSPAGFAT
                     RSTTSAFATRPSTTW"
     gene            2130..2354
                     /locus_tag="VNU03_22485"
     CDS             2130..2354
                     /locus_tag="VNU03_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77309.1"
                     /translation="MIGGKLRFGLLSGDPSGITFHSSAQRTAWLTGQRREVDLSEAHT
                     MWSIRSHEYQQCQRAGGSSCINPGPDPARR"
     gene            2450..3451
                     /locus_tag="VNU03_22490"
     CDS             2450..3451
                     /locus_tag="VNU03_22490"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77310.1"
                     /translation="MPARLALVLGVLAFFIAPDGAGAADKVTVALDFTLSGYHAPWFV
                     AQDKGYFAEQGLDVNLGRGYGSGDTVKKLATGAIDIGLNHPAPLIIANAEGGNLRTVM
                     GYLNQEMCAMYSSAEGGNVRVPKDLEGQTFGGPPGDICTMMLPALAEKTGVDYSKIKV
                     QQMDGPQRLAMLTAGRISVLGTFFDKDILIKKGMDQAGKTMVSFHYSQYISMYSIAVT
                     ATQATIDKRPEMVQKMVTALLRGFKFTVANPAEAVKITSKLHPELDADYVKASVDTLL
                     EGMWDETSKAKGIGILDAGKMQATRDTVVKYWKLKAEPPMAELYTNRFVEAAHKAVK"
     gene            3452..4228
                     /locus_tag="VNU03_22495"
     CDS             3452..4228
                     /locus_tag="VNU03_22495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77311.1"
                     /translation="MRERLGGAIAPTLAFAAVLVLWQGSVWLLRPPPFILPGIGQVIA
                     RLVAFGPNWAGHVVATIEGIALGFLAAVVFGTAGAVLIVSSDTLRRAITPLLVTIQIV
                     PKIAFAPLILVWFGIGLTSRATIAFLVAFFPVLVNTAVGLTQIEPDLLDLARSIKARP
                     AWVFFRIRFPSSLPYFFAGLRVASTLAVIGAVIGEFVGSDVGLGYLIVIANNQIDTAL
                     GLAAIFLISLIGLGLYAAVVLTERLCTPWAGGEGGLETSL"
     gene            4228..>4511
                     /locus_tag="VNU03_22500"
     CDS             4228..>4511
                     /locus_tag="VNU03_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP77312.1"
                     /translation="MAAFIEIRDVEKRYATRSGPQVALRPTSLEIAAGEFVSIVGPSG
                     CGKTTLMKIVAGLLPCTRGEVRIDGVPVQEPPDGVGMVFQTPTLLRWRTVL"
BASE COUNT          590 a         1613 c         1628 g          680 t
ORIGIN      
        1 ggggccggtc tcccgccgct gagcgcgatc gcggcctgcc agatctcgat ccgcagggcg
       61 tgcgcgtcgt cgagcaccgc cgccgcggcg gccgcccgct tcacgccgcg cgcccggagc
      121 cgcgcgaccg cggccgcggg cagcgtgccg cgcatccggc tccaggcgag cagggtggcg
      181 tagtcgggga tcagctcctg gggagacgcg gaggtgcgcc agtccaccgt gttggcgaag
      241 tccaggcaga cgcgcccgcc gaagaactgc gagcgggcgc ggaccggatc atttgacatc
      301 gagcgtccct cgacttctaa atgcccttga ccatgagaga acccggcgcg gcccggccgc
      361 cgttcaccgc ctccgcgttc cggcgcgggt tcgtggccgc gctgcccttc gtggtcagca
      421 acggggtcgg cggggtggtc atgggcgtcg cctatcgagg cctggggctc gacttctcgt
      481 cggccgttct ctggagcctc gtggtctatt ctggcacggc ccaggcggtc acgctcggcc
      541 tctggacgac gtcgccgccg atcgcggcga tggtggtcgc ctgcgtggcc acgaacgccc
      601 gctacctggt gctgggcgcg catctgcgtc agctgttcgg cgacgtgccc cggcgcgtga
      661 tgctgccgat cctggccctg ctggccgacg cctcgtggct catgacggtg gcggaggccg
      721 agcgcgggcg gcgggacgcg ggatacctgc tcggcagcgg cgtgccgatg gccatcggct
      781 ggatcggcgg caccgcgctc ggccacgcgc tgccgctggc gccgcgcggt cccctggcag
      841 tggccgccgc gttcctgccg ctggcgttcg tcgtcgtgct cctgcccacc cagtggtcgg
      901 gcgcgcggag cctgctgccg tggaccgtgt cggcggcggt ggcgctggcg gtggccggta
      961 cgctcggccc gagctgggcc atgctgatcg gcgggggagc cggcacgctc gtcagcgccc
     1021 tgcgcggcga tgcctgactc gcgcgtcctg gccgcgatcg tcgcggtcgg cctcgccagc
     1081 tacgcgatgc gggccggcgg cttcctcgcg gcgggcgcga tcccgcccgg cggcctgctg
     1141 gagcgcctcc tgcgcctggc gccgggcaac ctgttcgtcg cgttcgccgc cgccggcatg
     1201 ctcgagggcg gctggccgag tctcgtcggc tgcctcggcg cggtcgcgac gatggcggcc
     1261 accgatcggg aatgggcggc gctcggggcc ggcttcgcgg ccgcggccct cgcgtccacc
     1321 gtgctctgac cggccgcgcc gtcacccgcg cgcgggtcgg ccgtcgcgat gggtgtcacc
     1381 ttcgggtccg ggtcgcggcg gaccggaccg cggcgtgcta ccgtcgggcc catgggacga
     1441 ctcgcgcgcc ggctggggct gatcaccatc gtggccgtcg cgtgcgccgc gccggtgccc
     1501 ccggcatcgg cggcggaccc ggtcgtgctg gcgtccggcg cgctccagcg ttgcctcgga
     1561 atcacgcccg gccaggcgaa attcaacggt atgtcggcgg cgggcgcgcc gtactgcgag
     1621 cagctgcccg gtgcctccgc gtacgcccag gcggggcagc ggtaccaggc cggtgatcgg
     1681 gccggcgcag tcaagctcgt gaccagcgcc gcccaggcgg gacatccgct ggcccagctg
     1741 aggctcgcca tcatgtacga gggggcggac ggcgtgcccc gggacaagaa ggcggcgttc
     1801 acctggtacg cgcgggccgc ggcggtcggc gagcccgccg cgcaaaggga gctggccggc
     1861 tactacgaag acggggacgg ggtcgcggag aactgggatc tggccgcgaa gctgtaccag
     1921 gcgagcgcca cgcaagggtg gagcaagggc cagctcgcgc tgggccgggc ctacgagttc
     1981 ggcatcggtg tgccgcagga tcggcagcag gccgtcgcgt ggttcgcccg ggcgggcccc
     2041 agggactcgg ccagggcgag tacttcgccc gctggcttcg cgacccgctc aacaacatcg
     2101 gctttcgcaa ccaggccgag cacgacctgg tgatcggcgg caagctgcga ttcggcctgc
     2161 tctccgggga ccccagcggc atcacgttcc acagctccgc ccagcgcacc gcctggctga
     2221 cgggtcagcg ccgggaggtg gatctctccg aggcgcacac gatgtggagc atccggtccc
     2281 acgagtatca gcagtgccag cgggccggcg gaagcagctg catcaatccc gggcccgatc
     2341 cggctcgtcg ctagctcgct ccggggccgc ggggccgctt gacccgcgtg gaggcggtcg
     2401 gctacgctcc gggcacggaa ccgcgatccc gcagcgcagg agaccatgca tgcccgccag
     2461 actcgcgctc gtcctcggtg ttctcgcctt cttcatcgcc cccgacggcg cgggcgcagc
     2521 cgacaaggtc acggtcgctc tggacttcac gctcagcggg taccacgcgc cctggttcgt
     2581 ggcgcaggac aagggctact tcgcggagca gggcctcgac gtcaacctgg ggcgcggcta
     2641 cggctccggg gacacggtca agaagctcgc gaccggggcc atcgacatcg ggctcaacca
     2701 tccggcgccg ttgatcatcg ccaacgcgga gggcggtaac ctccggacgg tgatgggcta
     2761 cctcaaccag gagatgtgcg cgatgtactc ctccgccgaa gggggcaacg tgcgggtgcc
     2821 caaggacctc gagggccaga ccttcggcgg ccccccgggc gacatctgca cgatgatgct
     2881 cccggcgctg gccgagaaga ccggggtcga ctacagcaag atcaaggtcc agcagatgga
     2941 cggcccccag cgcctcgcga tgctcaccgc gggccgcatc tcggtgctcg gcaccttctt
     3001 cgacaaggac atcctgatca agaagggcat ggaccaggcg ggcaagacga tggtgagctt
     3061 ccactattcc cagtacatct cgatgtactc gatcgcggtc accgcgaccc aggccaccat
     3121 cgacaagcgc ccggagatgg tccagaagat ggtgaccgcg ctgctccgcg gcttcaagtt
     3181 caccgtcgcc aacccggcgg aggcggtgaa gatcacgagc aagctccacc ccgagctcga
     3241 cgccgactac gtgaaggcct cggtcgacac cctgctcgag ggcatgtggg acgagacctc
     3301 gaaggccaag ggcatcggca tcctggacgc gggcaagatg caggcgaccc gcgacacggt
     3361 ggtgaagtac tggaagctca aggcggagcc gccgatggcc gagctctaca cgaaccggtt
     3421 cgtcgaggcc gcccacaagg ccgtcaagta gatgcgcgag cggctcggcg gcgcgatcgc
     3481 gccgacgctg gcgttcgcgg cggtgctcgt gctctggcag ggctcggtct ggctcctgcg
     3541 gccgccgccc ttcatcctgc ccggcatcgg gcaggtgatc gcccgcctgg tggcgttcgg
     3601 acccaactgg gcgggccacg tggtcgccac catcgagggc atcgcgctcg gcttcctcgc
     3661 cgcggtcgtg ttcggcaccg cgggcgcggt gctcatcgtg tcgtcggata cgctgcggcg
     3721 ggcgatcacc ccgctcctgg tcacgatcca gatcgtgccc aagatcgcgt tcgcccccct
     3781 catcctggtc tggttcggga tcggcctcac ctcgcgggcc accatcgcgt tcctggtcgc
     3841 gttcttcccg gtcctggtca acaccgcggt cggcctcacc cagatcgagc ccgatctgct
     3901 cgacctggcc cgctcgatca aggcccggcc cgcgtgggtc ttcttccgca tccgcttccc
     3961 cagctcgctg ccctacttct tcgccgggct gcgggtcgcc tcgacgctcg cggtcatcgg
     4021 cgcggtcatc ggcgagttcg tgggctcgga cgtggggctc ggctacctga tcgtcatcgc
     4081 caacaaccag atcgacaccg cgctcgggct cgcggccatc ttcctgatct cgctcatcgg
     4141 cctcggcctg tacgcagccg tcgtgctcac cgagcggctc tgcaccccgt gggcgggcgg
     4201 cgagggcggg ctcgagacct cgctctgatg gccgccttca tcgagatccg cgacgtcgag
     4261 aagcggtacg cgacccggtc cggcccgcag gtcgcgctcc ggccgacctc gctcgagatc
     4321 gcggcggggg agttcgtgtc gatcgtggga ccgagcggct gcggcaagac cacgctcatg
     4381 aagatcgtgg ccggcctgct cccgtgcacg cgcggagagg tgcggatcga cggagtcccg
     4441 gtgcaggagc cgcccgacgg ggtggggatg gtgttccaga ctcccaccct cctgcgctgg
     4501 cgcacggtgc t
//
LOCUS       DATMCV010000984         5224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_589301, whole genome shotgun sequence.
ACCESSION   DATMCV010000984 DATMCV010000000
VERSION     DATMCV010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589301"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VNU03_22505"
     CDS             <1..672
                     /locus_tag="VNU03_22505"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWP77313.1"
                     /translation="MMVNVVRNGTGHNAAIPGYDVAGKTGTAQKMDPATRRYSRAPGV
                     LSFVGFVPADDPRLAMIVLLDEPKNEKWGSEAAAPIFAAIGREALRYLNVAPRDSSPV
                     PIVRAELARATPERPRAGASAEGPALDAVALPAGAADAASASAAQPAQPDGIMPRLGG
                     LSLRQAMETLAPHGVRLEITGRGVVTSQLPLPGAPLPPGTVCRLQLAPTAGRPTAALA
                     VSFPQ"
     gene            672..2192
                     /locus_tag="VNU03_22510"
     CDS             672..2192
                     /locus_tag="VNU03_22510"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWP77314.1"
                     /translation="MPTTTLLDALPDKTVLGSLPAGVTGVAYDSRKVAAGALFVAIPG
                     FKQDGRKFAADALGRGAAVVVAEGADPLPGSAAARVLVPSSREALARLADAYHGHPSR
                     RLTVIGITGTNGKTTTSLLVEALLGTGGRPTGVIGTIQYRVGDQAEAASQTTPEALEL
                     QALLARMVDAGVGGAAMEVSSHALFLSRVDGIEFDVAVFTNLTQDHLDFHKTLEAYRD
                     AKARLFGLLARSRKARRAAVVNVDDPAGAAMVRAAAADPRVRILTFGLRAPAELRPLR
                     WQSGMDGIRLEVASPGGAIEITSALVGEHNVMNLLGAVGVGLALEMAPVQIGRILSGV
                     ATVPGRFERVEAGQPFLVVVDYAHTPDALENVLATARKLLEPGARLGVVFGCGGDRDR
                     GKRPIMGGIAARLADRAWVTSDNPRSEQPEAILAEIETGIPATAAGRHESIADRRRAI
                     EGAVAWARKGDVVVIAGKGHETYQIVGSEVLPFDDREVARAALVARGGTGGRAWRA"
     gene            2180..3568
                     /gene="murF"
                     /locus_tag="VNU03_22515"
     CDS             2180..3568
                     /gene="murF"
                     /locus_tag="VNU03_22515"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWP77315.1"
                     /translation="MAGLSVEDVVRGTQGALVAGDLGVPVTGVSIDSRNLAIGEAFFA
                     IQATRDGHDFLRDAAARGASCLVVHNLPDDLPPSVPTILVDDTTRALGRLAAYHRARF
                     TVPVAAVTGSNGKTTTKEMMAAVLQALGPVLKPEGSFNNQWGLPLTLLRLGPEHRALA
                     LELGANTPGEIESLAAICRPTVGVVTVVQGAHTEAFRDLDGVAVEKSALVRAIPPDGT
                     VVLNADDPRVLAMRDGSRARVLYYSTRPGADVHAAGPASDAGDGLRFSLAIGTAQRPV
                     RMHFAGRHNVTNALAAAGVGLALGLSLEQIAQGLEAARPAKGRCVWRPAGRLRILDDT
                     YNANPGSVAAALETLAAPSDVHRRVVVLGDMLELGDISEAAHREMGRAVAASGAAEFL
                     GMGRWARVAVEAARQAGLAESHHVTTFEDAVALLLKRLAPGDAVLVKGSRGMRMERVV
                     DALVARFGGEDG"
     gene            3568..4650
                     /gene="mraY"
                     /locus_tag="VNU03_22520"
     CDS             3568..4650
                     /gene="mraY"
                     /locus_tag="VNU03_22520"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWP77316.1"
                     /translation="MLYELLVPLAKYHTAFNVFRYITFRTAMATVTALVISFILGPWL
                     ITRLREMQHGGSSVREDTPERHQKTKAGTPTMGGIGILVAILVATLLWANLRNRYIWV
                     LVLATAGLGLIGLKDDWKKLKTRKGISAREKFGAQIVLVGVLMGWLYVWPPDGFTTSV
                     VIPFFKGWLLTFGWLWIPFAILVIVGASNAVNLTDGLDGLAIGPIVTAGGAFAVIAYL
                     TGNFRAAEYLRILNVKGTGELTIFCGALVGAALGFLWFNSYPAQVFMGDVGSLSLGGS
                     LGTLAVVTKSELLLPLIGGIYVVEAASVIIQVASFKLTGRRVFRMAPLHHHYEIKGWA
                     EPKIIVRFWIVSIMLALLALTTLKLR"
     gene            4671..>5224
                     /locus_tag="VNU03_22525"
     CDS             4671..>5224
                     /locus_tag="VNU03_22525"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWP77317.1"
                     /translation="MATLRRDAAYQVGIFVSGDGTRGVPYRESLAGKTVAVVGLAKSG
                     VAAARLLRRLGARVLASDSGPLESLATEARVLEREGCALWTGGHPEAAFLGAELVVVS
                     PGVPLELPALAAVRARGVPVIGELELAWRVMEADCIAITGTNGKTTTTTLTGELLRTQ
                     VRPVMVGGNIGTPLAEHALEFPGDG"
BASE COUNT          665 a         1886 c         1949 g          724 t
ORIGIN      
        1 atgatggtga atgtggtgcg caacggcacc ggccacaacg cggccattcc gggctacgac
       61 gtggcaggca agaccggcac cgcgcagaag atggaccccg ccacccggcg ctactcgcgc
      121 gctcccggcg tgctctcgtt cgtgggcttc gtgcccgccg acgatccgcg cctcgccatg
      181 atcgtgctgc tggacgagcc gaagaacgag aagtggggga gcgaggccgc ggcgcccatc
      241 ttcgccgcga tcggccgcga ggccctgcgc tatctcaacg tggccccgcg cgactcgagc
      301 ccggtgccga tcgtgcgggc ggagctcgcc cgcgccaccc cggagcgccc gcgggccggc
      361 gcctccgcgg aaggccccgc gctcgacgcg gtcgcgctgc cggccggcgc cgcagacgcc
      421 gcctccgcgt cggcggctca gcccgctcag ccagatggaa tcatgccgcg gctcggcggc
      481 ctctcgctcc ggcaggcgat ggagaccctg gccccgcacg gggtgcggct cgagatcacc
      541 ggccgcggcg tggtgacgag ccagctcccg ctccccggtg ccccgctgcc gcccggcacg
      601 gtctgccggc tgcagctcgc gcccacggcg gggcggccga ccgccgccct cgcggtgagc
      661 ttcccgcagt gatgccgacc accaccttgc tcgacgcgct gccggacaag acggtgctgg
      721 gctcgctccc cgccggggtg accggcgtgg cctacgactc gcgaaaagtc gcggcgggcg
      781 cgctcttcgt ggcgatcccc ggcttcaagc aggacgggcg gaagttcgcc gccgacgcgc
      841 tcggccgcgg ggcggcggtg gtggtggccg agggcgccga tcccctgccg ggcagcgcgg
      901 cggcccgggt cctggtgccc tcctcgcgcg aggcgctcgc gcggctcgcg gacgcctacc
      961 acggccatcc gtcgcggcgc ctcaccgtga tcggcatcac cggcaccaac ggcaagacca
     1021 ccacgtcgct gctggtcgag gcgctgctcg gcacgggcgg gcggcccacc ggcgtgatcg
     1081 ggaccatcca gtatcgggtg ggagatcagg ccgaggccgc gagccagacc acgcccgagg
     1141 cgctcgagct gcaggctctc ctggcgcgca tggtggacgc gggagtgggc ggcgccgcga
     1201 tggaggtgtc gtcccacgcg ctcttcctct cgcgggtgga cggcatcgag ttcgacgtgg
     1261 ccgtcttcac caacctcacc caggaccatc tcgacttcca caagaccctc gaggcgtatc
     1321 gcgacgcgaa ggcccggctg ttcggcctgc tcgcgcggag ccgcaaggct cgccgcgccg
     1381 cggtggtgaa cgtcgacgat ccggcgggcg cggccatggt ccgcgccgcc gcggccgatc
     1441 cgcgggtgcg gatcctgacc ttcggcctgc gcgcccctgc cgagctccgg ccgctccgct
     1501 ggcagtccgg gatggacggg atccggctcg aggtcgcctc cccgggcggc gcgatcgaga
     1561 tcacctccgc gctggtgggc gagcacaacg tgatgaacct gctcggggcg gtgggcgtcg
     1621 gcctggccct cgagatggcg cccgtgcaga tcggccgcat cctctccggg gtcgcgaccg
     1681 tcccggggcg cttcgagcgc gtggaggccg gccagccgtt cctggtggtg gtcgactacg
     1741 cccacacccc ggacgcgctc gagaacgtgc tcgccaccgc gcgcaagctg ctcgagccgg
     1801 gcgcgcgcct cggcgtggtg ttcggctgcg gcggcgaccg ggaccgcggc aagcgcccga
     1861 tcatgggcgg gatcgccgcg cggctggccg accgggcgtg ggtgacttcc gacaacccgc
     1921 gcagcgagca gccggaggcc atcctggccg agatcgagac cggcatcccc gcgaccgcgg
     1981 cgggccgcca cgagtccatc gcggaccggc ggcgggccat cgagggcgcg gtcgcctggg
     2041 cccgcaaggg cgacgtggtg gtcatcgcgg gcaaggggca cgagacctac cagatcgtcg
     2101 ggtcggaggt gctgccgttc gacgaccgcg aggtggcccg ggccgcgctt gtcgcgcgcg
     2161 ggggaaccgg agggcgcgcg tggcgggcct gagcgtcgag gacgtggtcc gcggcaccca
     2221 gggcgccctg gtggcgggcg acctcggggt gccggtgacc ggggtcagca tcgacagccg
     2281 caacctggcc atcggcgagg ccttcttcgc gatccaggcc actcgcgacg gccacgactt
     2341 cctgcgggac gccgcggcgc ggggcgcctc gtgcctggtc gtccacaacc tgcccgacga
     2401 cctgccgccg tcggtgccga cgatcctggt ggacgacacc actcgtgcgc tcgggcgcct
     2461 cgcggcctat caccgggccc gcttcacggt cccggtcgcg gcggtcaccg gctccaacgg
     2521 caagaccacc accaaggaga tgatggccgc ggtgctgcag gcgctcggcc cggtgctcaa
     2581 gcccgagggc agcttcaaca accagtgggg gctgccgctc accctgctcc gcctgggccc
     2641 cgagcaccgg gcgctcgcgc tcgagctggg cgccaacacc ccgggcgaga tcgagtcgct
     2701 cgccgcgatc tgccgtccca cggtgggcgt ggtcacggtg gtgcagggcg cccacaccga
     2761 ggccttccgc gatctcgacg gagtggccgt cgagaagagc gcgctcgtcc gcgcgatccc
     2821 gccggacggc acggtggtgc tcaacgcgga cgatccgcgg gtgctcgcga tgcgggacgg
     2881 cagccgcgcg cgggtgctct actacagcac gcggcccggc gccgacgtcc acgccgcggg
     2941 ccccgcctcc gacgcgggcg acgggctccg cttcagcctc gcgatcggca ccgcgcagcg
     3001 cccggtgcgg atgcacttcg cgggccgcca caacgtcacg aacgcgctcg ccgcggcggg
     3061 ggtggggctc gccctcggcc tctcgctcga gcagatcgcg cagggcctgg aggcggcacg
     3121 gccggccaag gggcgctgcg tctggcgccc ggccggccgc ctgcgcatcc tcgacgacac
     3181 ctacaacgcc aatcccggct cggtggccgc cgcgctcgag accctcgccg cgccgtcgga
     3241 cgtgcaccgg cgcgtggtgg tgctgggcga catgctcgag ctgggcgaca tcagcgaggc
     3301 cgcgcatcgc gagatgggcc gggcggtggc cgcctccggc gcggcggagt tcctcggcat
     3361 gggccgctgg gcgcgggtgg cggtggaggc ggcgcggcag gccgggctcg cggagagcca
     3421 tcacgtgacc acgttcgagg atgcggtggc gctgctgctc aagcggctgg cgccgggcga
     3481 cgcggtgctc gtcaagggct cccggggcat gcgcatggag cgtgtggtgg acgcgctcgt
     3541 ggcccgcttc ggaggagaag atggctgatg ctctacgagc tgctggtgcc gctcgcgaag
     3601 tatcacaccg ccttcaacgt gttccggtac atcaccttcc ggaccgcgat ggcgacggtc
     3661 accgcgctgg tgatctcgtt catcctgggg ccgtggctga tcacccggct gcgcgagatg
     3721 caacacgggg gctcgtcggt ccgggaggac accccggagc ggcaccagaa gacgaaggcc
     3781 gggacgccca ccatgggggg catcggcatc ctggtcgcga tcctggtcgc caccctgctg
     3841 tgggccaacc tgcgcaaccg ctacatctgg gtgctggtgc tggccaccgc ggggctcggg
     3901 ctgatcgggc tcaaggacga ctggaagaag ctcaagaccc gcaagggcat ctcggcgcgc
     3961 gagaagttcg gggcccagat cgtgctggtg ggcgtgctga tgggatggct ctacgtctgg
     4021 ccgcccgacg gcttcaccac cagcgtcgtg atcccgttct tcaagggctg gctgctcacc
     4081 ttcggctggc tctggatccc gttcgcgatc ctggtgatcg tgggcgcgtc caacgcggtg
     4141 aacctgaccg acgggctgga cgggctcgcc atcgggccga tcgtgaccgc gggcggggcc
     4201 ttcgcggtga tcgcgtacct caccggcaac ttccgggccg ccgagtacct gcgcatcctg
     4261 aacgtcaagg gcaccggcga gctgacgatc ttctgcggcg cgctggtggg cgccgcgctc
     4321 ggcttcctct ggttcaacag ctacccggcc caggtgttca tgggcgacgt gggctcgctg
     4381 tcgctcggcg gctcgctcgg caccctcgcg gtggtcacca agtcggagct cctgctgccg
     4441 ctcatcggcg gcatctacgt ggtggaggcg gcctcggtca tcatccaggt cgcctccttc
     4501 aagctgaccg gacgacgggt ctttcgaatg gcgccgttac accaccacta cgagatcaag
     4561 ggatgggcgg agcccaagat catcgtgcgg ttctggatcg tgtccatcat gttggcgctc
     4621 ctggccctca ccactctcaa gctccgctag ggtgacatga catggcgatt ttggcgacac
     4681 tgcggcgcga cgccgcgtat caggtgggga tcttcgtgag cggcgacggg acgcgcggcg
     4741 tgccgtaccg cgagtcgctc gcgggcaaga cggtggcggt ggtgggcctc gccaagagcg
     4801 gggtggccgc cgcgcggctg ctgcgccggc tcggggcgcg cgtgctcgcc tccgactccg
     4861 gtccgctcga gagcctcgcc accgaggcgc gggtgctgga gcgcgagggc tgcgcgctct
     4921 ggaccggcgg ccacccggag gccgcgttcc tgggcgcgga gctggtggtg gtgagcccgg
     4981 gcgtgccgct cgagctgccc gcgctggccg cggtccgcgc ccgcggggtg ccggtcatcg
     5041 gcgagctcga gctggcctgg cgggtgatgg aggccgactg catcgcgatc accggcacca
     5101 acggcaagac caccaccacc acgctcaccg gcgagctgct gcgcacccag gtgcggccgg
     5161 tgatggtggg cggcaacatc ggcaccccgc tcgccgagca cgcgctcgag ttcccgggcg
     5221 acgg
//
LOCUS       DATMCV010000985         3471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_718007, whole genome shotgun sequence.
ACCESSION   DATMCV010000985 DATMCV010000000
VERSION     DATMCV010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718007"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..1255
                     /locus_tag="VNU03_22530"
     CDS             365..1255
                     /locus_tag="VNU03_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP77318.1"
                     /translation="MPFADVAGVKLFYEETGEGAPLIFVHEFAGDYQSWHLQVRFFAR
                     RYRTIAYNARGYPPSDVPDKPEAYSQVQAAEDILGILDHLKISKAHICGLSMGGYAVL
                     HFGLRHPQRALSLTVAGAGYGSVPGERERFRKDVDETARRFETDGMAGVAGFYTKGPT
                     RVQFMDKDPKGWQEFYDQFAAQSAEGHALTMRGVQMSRPSIFDLEAAISHLEVPTLIM
                     TGDEDDPCLEPAIFMKRKIRSSGLVVIPKAGHTINLEDPDLFNRALLDFLTAVDAGRW
                     PLRNPQSQTGSAIMPAGGSR"
     gene            1252..2418
                     /locus_tag="VNU03_22535"
     CDS             1252..2418
                     /locus_tag="VNU03_22535"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP77319.1"
                     /translation="MTLPLEPYIVIDLTRARSGPTCVRQLADMGARVVQISAREDVEG
                     DFPRRGFDSQNLHRNKRSLTLDLKSPRGIEIIHKLVKTADVVVENFRPDVKTRLGIDY
                     ETLSRINPRLVYGSISGFGQDGPYRERPGYDQIAQGMGGLMSITGLPGQGPVRVGIPV
                     ADLTAGIFLAQGILVALLERERSGKGQWVHTSLLQAMVTMLDFQAARWLIGHEIPPQA
                     GNDHPTAIPTGVFKTADGHLNIAASGQHMFRRLCEALGTEPLLEDPRFRTAPDRSKNR
                     KALAVELEKGFAKRTTQEWVEVLNAKGVPSGPILDVKQVYEDEQIRHLKMAVPVKHPA
                     LGELLVQAPPVTLSRTPGAVRTPAPDTGEHNDQILTELGYSPSDIDTLRKDKVI"
     gene            2431..3222
                     /locus_tag="VNU03_22540"
     CDS             2431..3222
                     /locus_tag="VNU03_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWP77320.1"
                     /translation="MRENALRKIWAKGEAVVNGWLSIPSGFSAEVMAHQGFDSLTIDM
                     QHGVVDYQVAVSMLQGISTLGVVPLARVPWNDPARLMKILDAGAYGVICPMINTAQEA
                     EAMVRACKYPPRGFRSWGPVRASLYAGGDYGDHANVETIVMPMIETAEALKNLDEILS
                     VDGVDAIYVGPSDLSLAMGLKPRLDQTDPPVVEAQKKIAAACKRHNVIAGIHNATAAY
                     SLKMIAEGYQFVTIASDSRFMAAKAAEEVGVVRKTGTKAGKLPAY"
     gene            3222..>3471
                     /locus_tag="VNU03_22545"
     CDS             3222..>3471
                     /locus_tag="VNU03_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWP77321.1"
                     /translation="MPARLDGALLDRARANLAAFERLALPDDGRRRAAVAEVLLPDED
                     GRPCFLITRRAATLRQHTGQWALPGGRVDAGETAERAAL"
BASE COUNT          582 a         1261 c         1142 g          486 t
ORIGIN      
        1 cgcgacgacg tccgcgagac ccccgccgct gttataggcg ggaatgacga tagagagacg
       61 caccgggccc tcgatccacg ttgatccggc tctccggcct accccccacg ggcttcgccc
      121 tcctcgcagg cagcacggtc acctgggacc gccccgggtc gtcctccgcc ccggggtaac
      181 accccggggg ttgctgctgg ttagcatacg tcgtgccgag ggagaatccg aagtttaaca
      241 agcccttccg ttacgggcga tccaccactt cgcgagacct tgtaaaaact gccggcataa
      301 aggtgccggc cgggtctgtt gtacaatccg caggggccgt ccacaggggc cgaggagccg
      361 agccatgcca ttcgccgacg tcgcgggagt caagctgttc tacgaggaga cgggagaggg
      421 cgcgcccctg atcttcgtcc acgagttcgc gggcgattac cagagctggc acctgcaggt
      481 gcgcttcttc gcccgccgct accgcaccat cgcctacaac gcgcgcggct acccgccttc
      541 cgacgtgccg gacaagcccg aggcctactc gcaggtacaa gccgccgaag acattctggg
      601 aatcctcgac cacctcaaga tctccaaggc gcacatctgc gggctgtcga tgggcggcta
      661 cgcggtgctg cacttcggcc tgcgccatcc ccagcgcgcg ctctcgctga cggtggcggg
      721 cgcggggtac ggcagcgtgc cgggcgagcg ggagcgcttc cgcaaggacg tcgacgagac
      781 ggcccggcgc ttcgagaccg acggcatggc cggggtggcc ggcttctaca ccaagggccc
      841 cacccgggtg cagttcatgg acaaggaccc gaagggctgg caggagttct acgaccagtt
      901 cgcggcccag tccgccgagg gccacgccct cacgatgcgg ggcgtgcaga tgtcgcgacc
      961 gtccatcttc gacctcgagg cggcgataag ccatttggaa gtcccgaccc tgatcatgac
     1021 cggcgacgag gacgatccgt gcctcgagcc cgccatcttc atgaagcgca agatccgctc
     1081 gtccggcctc gtggtcatcc ccaaggccgg ccacaccatc aacctcgagg acccggacct
     1141 cttcaaccgc gccctcctcg acttcctcac cgcggtggac gccggccgct ggccgctccg
     1201 caacccgcag tcgcagaccg gatccgccat catgcccgcc ggaggatcgc gatgacgctg
     1261 ccgctcgagc cctatatcgt gatcgacctc acccgcgccc gctcgggccc cacctgcgtg
     1321 cgccagctcg cggacatggg cgcgcgcgtg gtacagatct cggcgcgcga ggacgtggag
     1381 ggcgacttcc cgcggcgcgg cttcgactcg cagaacctcc accgcaacaa gcgctcgctc
     1441 accctcgacc tcaagtcgcc gcgcggcatc gagatcatcc acaagctcgt caagactgcg
     1501 gatgtcgtcg tggagaactt ccggcccgac gtcaagacgc ggctcggcat cgactacgag
     1561 acgctctccc gcatcaaccc gcggctggtc tacggcagca tctccggctt cggccaggac
     1621 gggccctatc gcgagcggcc gggctacgac cagatcgccc agggcatggg cggtctcatg
     1681 tccatcaccg gcctgcccgg ccagggcccg gtgcgcgtgg gcatcccggt ggccgacctc
     1741 accgcgggca tcttcctggc ccagggcatc ctggtcgcgc tgctcgagcg tgagcgctcc
     1801 ggcaagggcc agtgggtgca cacctcgctg ctccaggcga tggtgaccat gctcgacttc
     1861 caggccgcgc gctggctcat cggccacgag atcccgcccc aggccggcaa cgaccatccc
     1921 accgcgatcc ccaccggggt gttcaagacc gcggacgggc acctcaacat cgcggcgtcc
     1981 gggcagcaca tgttccggcg cctctgcgag gccctcggca ccgagccgct cctcgaggac
     2041 ccgcgctttc gcaccgcccc ggaccgctcc aagaaccgca aggcgctcgc ggtcgagctc
     2101 gagaaggggt tcgccaagcg caccacccag gaatgggtcg aggtgctcaa cgccaagggc
     2161 gtgccgtcgg gcccgatcct cgacgtcaag caggtctacg aggacgagca gatccgtcat
     2221 ctgaagatgg cggtgccggt gaagcacccg gcgctcggcg agctgctcgt gcaggccccg
     2281 ccggtcacgc tgtcccgcac cccgggcgcg gtgcgcaccc ccgcccccga taccggcgag
     2341 cacaacgacc agatcctgac cgagctcggc tactccccgt ccgacatcga cacccttcgc
     2401 aaagacaagg tcatctaaca ggagagtgat gtgagagaga acgcgctgag gaagatctgg
     2461 gcgaagggcg aggcggtggt gaacgggtgg ctgtccatcc cgagcggctt ctcggccgag
     2521 gtgatggcgc accagggctt cgactcgctc accatcgaca tgcagcacgg cgtcgtcgac
     2581 taccaggtcg cggtgagcat gctccagggc atctcgaccc tgggcgtggt gccgctggcc
     2641 cgggtgccgt ggaacgaccc cgcgcggctc atgaagatcc tcgacgcggg cgcctacggg
     2701 gtcatctgcc ccatgatcaa caccgcgcag gaggcggagg cgatggtacg cgcgtgcaag
     2761 tacccgccgc gcggcttccg gagctggggc ccggtgcgcg cctcgctcta cgccggtgga
     2821 gactacggcg accacgccaa cgtcgagacc atcgtgatgc cgatgatcga gaccgcggag
     2881 gcgctcaaga acctggacga gatcctgagc gtggacggcg tcgacgccat ctacgtgggc
     2941 ccttccgacc tgagcctggc catgggcctc aagccccggc tcgaccagac cgatccgccg
     3001 gtggtggagg cccagaagaa gatcgcggcg gcctgcaagc ggcacaacgt gatcgcgggc
     3061 atccacaacg ccaccgcggc ctactcgctc aagatgatcg cggagggcta ccagttcgtg
     3121 acgatcgcga gcgacagccg cttcatggcc gccaaggccg ccgaggaagt gggcgtcgtg
     3181 cgcaagaccg gcaccaaggc gggaaagctg ccggcctact agtgccggcc cggctggacg
     3241 gcgcgctgct cgaccgggcg cgcgccaacc tggccgcctt cgagcggctc gccctgcccg
     3301 acgacggacg gcgccgcgcc gcggtggccg aggtgctgct gcccgacgag gacggccgcc
     3361 cgtgcttcct cattacccgg cgggccgcga cgctgcgcca gcacaccggc cagtgggccc
     3421 tgccgggcgg gcgcgtcgac gcaggcgaga ccgcggagcg cgcggcgctg c
//
LOCUS       DATMCV010000986         3800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_990824, whole genome shotgun sequence.
ACCESSION   DATMCV010000986 DATMCV010000000
VERSION     DATMCV010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990824"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /gene="ruvB"
                     /locus_tag="VNU03_22550"
     CDS             complement(<1..879)
                     /gene="ruvB"
                     /locus_tag="VNU03_22550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWP77322.1"
                     /translation="MTDEARVMSRVALPDETQVERQLRPQRLEEYVGQRATVESLRVS
                     VEAARQRAEPLDHVLLSGPPGLGKTTLATIMANEMGASIVTTAGPSLERGADLMGILT
                     NLAERDVLFIDEIHRLPRVVEELLYPAMEDFSINFVMDKGLNARTMRIPLRPFTMVGA
                     TTRPGMLSSPLRERFGIFHHLDFYSDEDLAAIIRRSASILGMDVAPGGAEAIARRARG
                     TPRIANRLLRRVRDYAQVKADGVVTAPLAGRALDQEGVDEVGLDRLDRRFLTAIIEQY
                     KGGPVGLEAIAATINDE"
     gene            complement(876..1526)
                     /gene="ruvA"
                     /locus_tag="VNU03_22555"
     CDS             complement(876..1526)
                     /gene="ruvA"
                     /locus_tag="VNU03_22555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWP77323.1"
                     /translation="MIASLRGRLRRKLEDRIVVEAAGVGYEVFVPPVTQQELVHAKAA
                     EGDAADEVALAIHYHATQNQPRPVLIGFTSELDREFFEKLITVKDVGPLVAARSLAAP
                     VGEIAAAIARQDEGYLRRLPGIGPQKAKNIVAQLQAKVAKFALARPEAAVPEPSAAAP
                     PDEEGLRGMVFEILVKQLGHRPSEAAQLISAALSRRPGVTTPEELFDEIYRGGVRP"
     gene            complement(1523..2149)
                     /locus_tag="VNU03_22560"
     CDS             complement(1523..2149)
                     /locus_tag="VNU03_22560"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWP77324.1"
                     /translation="MGVDPGLVATGFGVLEAGAGGVTVRDAGVISTTTGQPLEARLNA
                     LHQAVYRIIELQSPGLLVVEDLYTEYKFPRTAILMGHARGVIYLAARQLGVAVVALAP
                     SEVKRAMTGNGAAGKAQVQRAVQTVLGLKELPRPSHVADALGLAATGMSRMTGRFPAA
                     VGGRTLGGFSSAAGAQKWPPHSPMSAVDRVRAAAVESARPAVNPGRPA"
     gene            complement(2163..2915)
                     /locus_tag="VNU03_22565"
     CDS             complement(2163..2915)
                     /locus_tag="VNU03_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWP77325.1"
                     /translation="MSGHSRWSQIKRKKGKTDVQRGKLFSKILREITVAAKNGGGDPK
                     GNLRLKAATESARAANMPADNIKRAIQKGTGELPGESYDEIAYEGYGAGGVAVMVRTL
                     TDNKNRTGPEIRHIFEKNEGRMGTAGCVAWMFDRRGMIEVDAQKVKEDDLLEKALESG
                     ATDVRQVEKVFEVTANPDEMETVRQALERQGMPVVEARVDMVPQSTVKVEGKEAAAVL
                     RLIEALEEQDDVQSVYSNYDIADEVIDAITAA"
     gene            complement(2925..>3800)
                     /locus_tag="VNU03_22570"
     CDS             complement(2925..>3800)
                     /locus_tag="VNU03_22570"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HWP77326.1"
                     /translation="IAASRVARGNVLLGALDDIDFLHPVKVGEVAVLRAQVEYVGRCS
                     LEVGVRVYAEKPATGERALTLSSHLVFVKVDEHGKPLPVPQAVLPRGAEEEALHAAAG
                     ERRARRLERFERRAERMREVDDDATPEQLRWTFESCRSVLPEDLLFGNTMFAGKLLLD
                     IDEAGGILSMRYCRGLVMTACLDALDFYAPIHANEVVMFKAGLNHVGTTSLEIGVKVL
                     SEVPWTGEVKHACTAYLSFVHLGSDPSAPLRPRPCPPFTPETPAEQRRFALALERRAR
                     RLERVKSLKRLSPSG"
BASE COUNT          504 a         1420 c         1294 g          582 t
ORIGIN      
        1 ctcgtcgttg atggtggccg cgatggcctc caggcccacc gggccgccct tgtactgctc
       61 gatgatcgcg gtgaggaacc ggcggtcgag ccggtcgagc ccgacctcgt ccaccccctc
      121 ctggtcgagc gcgcgcccgg ccagaggggc ggtgaccacg ccgtcggcct tcacctgcgc
      181 gtagtcgcgc acccggcgga gcagccggtt ggcgatgcgc ggcgtgccgc gcgcccggcg
      241 cgcgatggcc tcggcgccac ccggggcgac gtccatcccc aggatcgagg cggagcggcg
      301 gatgatcgcg gcgagatcct cgtcggagta gaagtccaga tggtggaaga tcccgaagcg
      361 ctcccggagc ggcgaggaga gcatgccggg gcgcgtcgtc gcgcccacca tcgtgaaggg
      421 ccggagcggg atgcgcatgg tccgcgcgtt gagccccttg tccatcacga agttgatcga
      481 gaagtcctcc atggccgggt aaagcagctc ctcgaccacg cggggcagcc ggtggatctc
      541 gtcgatgaac agcacgtccc gctcggccag gttggtgagg atccccatga ggtcggcgcc
      601 gcgctcgagg gagggccccg cggtcgtcac gatcgaggcg cccatttcgt tggccatgat
      661 ggtggcgagc gtggtcttgc caagccccgg cggcccggag agcagcacgt gatcgagcgg
      721 ctcggcccgc tgccgggccg cctcgaccga cacccgcagg ctctccaccg tcgcgcgctg
      781 gccgacatac tcttccaggc gctgcgggcg cagctgccgc tccacctggg tctcgtcggg
      841 cagcgccacc cggctcatca cgcgcgcctc gtcggtcacg gccgcacccc gccgcggtag
      901 atctcgtcga acagctcctc gggcgtggtc accccgggcc gacggctgag cgcggcgctg
      961 atgagctgcg ccgcctcgct cgggcggtgg ccgagctgct tgaccaggat ctcgaagacc
     1021 atgccgcgca gcccctcctc gtccggcggg gcggccgcgg acggctccgg gaccgccgcc
     1081 tccggccggg ccagcgcgaa cttggcgacc ttggcctgga gctgggcgac gatgttcttg
     1141 gccttctgcg gcccgatgcc gggcagccgt ctcaggtatc cctcgtcctg gcgcgcgatg
     1201 gcggccgcga tctcccccac cggcgcggcc agcgagcgcg ccgccaccag cggccccacg
     1261 tccttcaccg tgatcagctt ctcgaagaac tcgcggtcga gctccgacgt gaacccgatg
     1321 agcaccggcc gcggctggtt ctgggtggcg tggtagtgga tcgccagggc cacctcgtcg
     1381 gccgcgtcgc cctcggcggc cttggcgtgc accagctcct gctgggtcac cggcggcacg
     1441 aacacctcgt accccacccc ggccgcctcg accacgatcc ggtcctcgag cttccgccgc
     1501 agccgccccc gcagcgacgc gatcacgccg gccgccctgg attgaccgcg ggcctggcgg
     1561 actccaccgc tgctgcccgc acccggtcga ccgcggacat gggggagtgc gggggccatt
     1621 tctgggcccc cgcagctgaa gagaagcctc cgagcgtccg cccgcccacg gccgccggaa
     1681 acctcccggt catccgggac atcccggtgg cggcgagccc gagcgcgtcg gccacgtgcg
     1741 aggggcgcgg cagctccttg agtccgagca cggtctggac cgcgcgctgg acctgcgcct
     1801 tgcccgcggc gccgttgccg gtcatggcgc gcttgacctc cgagggggcg agcgcgacca
     1861 ccgcgacgcc cagctgccgc gcggcgaggt agatcacgcc ccgggcgtgg cccatgagga
     1921 tcgcggtgcg cgggaacttg tactcggtgt agaggtcctc gaccacgagg agaccggggc
     1981 tctgcagctc gatgatccgg tacaccgcct ggtggagcgc gttgagccgg gcctcgagcg
     2041 gctggccggt ggtggtgctg atgactccgg cgtcgcggac ggtgacgccg cccgcgcccg
     2101 cttccagcac gccgaacccg gtggcgacga gccccgggtc cacccccatg acgcgcagcg
     2161 cgctaggccg cggtgatggc gtcgatgacc tcgtcggcga tgtcgtagtt ggagtaaacg
     2221 gactggacgt cgtcctgctc ctcgagggcc tcgatcagcc gcagcaccgc cgcggcctcc
     2281 ttgccctcca ccttcaccgt ggattggggc accatgtcca cccgcgcctc gaccaccggc
     2341 atgccctgcc gctcgagcgc ctgccggacc gtctccattt catcagggtt cgcggtcacc
     2401 tcgaacactt tttctacctg acgcacgtcg gtcgcccccg actcgagcgc cttctcgagc
     2461 aggtcgtcct ccttcacctt ctgcgcgtcc acctcgatca tcccgcggcg gtcgaacatc
     2521 caggccacgc agccggcggt gcccatccgg ccctcgttct tctcgaagat gtgccggatc
     2581 tcggggccgg tgcggttctt gttgtcggtg agggtccgca ccatcaccgc caccccgccc
     2641 gctccgtagc cctcgtaggc gatctcgtcg tacgactcgc ccgggagctc gccggtgccc
     2701 ttctggatgg cccgtttgat gttgtcggcg ggcatgttgg ccgcccgcgc cgactccgtg
     2761 gccgccttca ggcgcaggtt gcccttgggg tcgccgccgc cgttcttggc cgcgacggtg
     2821 atctcccgca ggatcttcga gaagagcttc ccgcgctgga cgtccgtctt gcccttcttc
     2881 cgcttgatct gcgaccaccg tgagtgtcca gacatgccgg tctcctaacc gctcggggat
     2941 agccgcttca gtgatttgac ccgctccagc cgccgggccc gccgctcgag cgccagggcg
     3001 aagcggcgct gctcggccgg ggtctcgggc gtgaacggcg ggcacggccg cggacgcagc
     3061 ggcgcgctcg gatccgagcc caggtggacg aaggagaggt aggcggtgca ggcgtgcttg
     3121 acctcaccgg tccacggcac ctcgctcagc actttcaccc ctatctccag agaagttgtc
     3181 ccgacgtggt tgagtcccgc cttgaacatc acgacctcgt tcgcgtggat cggggcgtag
     3241 aagtcgagcg cgtccaggca ggcggtcatg accaggcccc ggcagtaccg catggagagg
     3301 ataccgcccg cctcgtcgat gtcgagcagg agcttgcccg cgaacatggt gttgccgaac
     3361 aggaggtcct ccggcagcac cgaccggcag gactcgaacg tccagcggag ctgctcgggg
     3421 gtcgcgtcgt cgtccacctc gcgcatgcgc tccgcgcgcc gctcgaagcg ctccagccgg
     3481 cgcgcccgcc gctcaccagc cgccgcatgc agcgcctcct cctcggcccc gcgcggcaac
     3541 accgcttgcg gaaccggcag gggcttgccg tgctcgtcca ccttcacgaa gaccaggtgg
     3601 gaggacagcg tcagggcccg ctcgccggtg gccggcttct cggcgtagac ccgcaccccg
     3661 acctcgaggg agcagcggcc cacgtactcc acctgggccc gcagcaccgc gacctccccc
     3721 accttcaccg ggtgcaggaa gtcgatatcg tcgagggccc cgagcagcac gttgccgcgg
     3781 gccacccgcg aggccgcgat
//
LOCUS       DATMCV010000987         4977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_121402, whole genome shotgun sequence.
ACCESSION   DATMCV010000987 DATMCV010000000
VERSION     DATMCV010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121402"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..943)
                     /locus_tag="VNU03_22575"
     CDS             complement(110..943)
                     /locus_tag="VNU03_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWP77327.1"
                     /translation="MAGGAGLIRRVALGGLGVLVAAALLAPYAVMLLTALKPEAELRV
                     TPPRLLPIAWRPQNFWTVLRDPEFQAWLRVSLVVAVASTAIVIAAAVPAAYHTARHRF
                     AGRGAFLFLVLVTQMFAPTALVVGLYREFFELGLINTYAALILVNAAFNLAFAIWILR
                     AFFASIPREIEEAAAVDGCGRAATLLRVVLPVALPGIVTAAIFAFIAAWNEYIVALTL
                     MTDASMRPLTVGITSYVTAYVQHWDLLFAASTIAIVPVVVFFALIEPYLVGGLTAGSV
                     K"
     gene            complement(922..1791)
                     /locus_tag="VNU03_22580"
     CDS             complement(922..1791)
                     /locus_tag="VNU03_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWP77328.1"
                     /translation="MGRALLWLGPSLLLIAAVVVYPAVQLVRASLGRYSITGLYQGAV
                     GARNYGRLLEQDALPTVIGNTVTWVALVVGITIVISLGLAQLLDATFPGRRLVRWALI
                     VPWAASLIMTSKLFAWLYDYYFGLINRALVAAGLVRAPVDWLGDDATVMGAMIAVGVF
                     VSLPFTTYVLLAGLSTIPAEVYEAARVDGAAGWRAYRAVTLPLLRPALLVAAVLNLIY
                     VFNSFPIVWTLNDRNPGMAHDTMITYMYKIAFKSALRDVGLAAALGVVNVVLILLAVL
                     AYLRVSRWREEPV"
     gene            complement(1836..3068)
                     /locus_tag="VNU03_22585"
     CDS             complement(1836..3068)
                     /locus_tag="VNU03_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWP77329.1"
                     /translation="MTSRRLLVLALLCVTAVVTVACGRGDGKPATIKVVIAEYSRDHT
                     RPFWQSLAEQYTKQTGVKVDLQVIDWNSIDQQVSTMIQNNQPPDVLNLNSFSSYAKDG
                     LLYSADEALSPKTRDDFLEAFARGGTYRDKLYGFPILASARAFFYNRDLLERAKVNAP
                     PRTWDELVQAARKVQALGGGVIGYALPLGPEEAQAEWSIWMWNNGGDWKSGDAWAINS
                     DKNVQTLQFLAGLANTHKVTQVNPGKTNRTDGAFQLFKDGKVGMVMGFSPLAAQLDAE
                     GKVRYGVTQMPTNVGAPATLGVEDYLMAFKKKGNQEAVRQFLDLYYQPEQITRWITAE
                     GFLPVTKSGRERMSGNEKLKPYLDALPSAHLAPTTDPVWDKVKLDVQQTIGLAVQPGG
                     DPKQVLDRLQKNAAESGR"
     gene            3217..3627
                     /locus_tag="VNU03_22590"
     CDS             3217..3627
                     /locus_tag="VNU03_22590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77330.1"
                     /translation="MARVLILFTLVLTLAACASSSGPAKPQRSEFEDVPVPAGLTLDS
                     NRTTIIESPTVKAARLFYKSRIKPDSLSVAYRTSLEANGWRHVSSTTSAGKGTTQVYE
                     KQDSSLQVLIYEGWYYTWTEVSTTRITARPAPAK"
     gene            3573..4250
                     /locus_tag="VNU03_22595"
     CDS             3573..4250
                     /locus_tag="VNU03_22595"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWP77331.1"
                     /translation="MDRGLNYPDHRSPGPRQVGAIGSRLLACVLLLGGCAATQSAYAP
                     VPARDASVDDRVTAVEGRLGEMNQRLDQVGGQVQRLEGGVTTAQGRADEAAKKAEGVD
                     GRLTRLWTNRFNQKQADSLEVYFSPDSIDLTDAAQTALLAVVREMEAHPTLTVELGGY
                     TDPRGALDYNYGLSQRRVDAVRRFLMDKGIQLARIQAASLGPIATAGIPDVKKRRVTV
                     RLMADPE"
     gene            4367..4675
                     /locus_tag="VNU03_22600"
     CDS             4367..4675
                     /locus_tag="VNU03_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HWP77332.1"
                     /translation="MTTYRMLATSAVALLMAGCVVAQPVVVRRPPPPPPVRVEIAPVA
                     PTPAHVWIPGHWGWHREHYVWVPGHWEVPASPGYVWVPGGWTPREGGYVWVEGHWRAR
                     "
     gene            complement(4700..>4977)
                     /locus_tag="VNU03_22605"
     CDS             complement(4700..>4977)
                     /locus_tag="VNU03_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77333.1"
                     /translation="PWDLSPGSTMTNATVDAMVERVEGPILTLKHKGGSVTVAVPTDV
                     PIVTFAPGDKAMLQPGAHVFVPAQQQPDGTVTAARVLVGKDGLVPPM"
BASE COUNT          780 a         1735 c         1762 g          700 t
ORIGIN      
        1 ctcgatcgag acggcggggc gggatgcgag tcagcgggtc cgggaagccc cccccgcccc
       61 cggcccaccc ccacccgccg agccgagcgc gagcgcgctg gaccgggagc tacttgacgc
      121 tacccgcggt gagcccgccg acgaggtagg gctcgatgag ggcgaagaac acgacgaccg
      181 ggacgatcgc gatggtggag gcggcgaaga ggaggtccca gtgctggacg taggcggtca
      241 cgtaggaggt gatgccgacg gtgagggggc gcatcgaggc gtcggtcatc agggtgaggg
      301 cgacgatgta ctcgttccag gccgcgatga aggcgaagat ggcggcggtg acgatgccgg
      361 gcagcgcgac cggcaggacg acgcggagca gggtcgcggc gcgaccgcac ccgtcgaccg
      421 cggcggcctc ctcgatctcg cgcgggatcg aggcgaagaa ggcgcgcagg atccagatcg
      481 cgaaagcgag attgaacgcg gcgttgacca ggatcagcgc ggcgtaggtg ttgatcaggc
      541 ccagctcgaa gaactcgcgg tagaggccga cgaccagcgc ggtcggcgcg aacatctggg
      601 tcaccaggac caggaacagg aacgcgccgc ggcccgcgaa ccgatgccgc gcggtgtggt
      661 aggccgcggg caccgcggcc gcgatcacga tggcggtcga ggccaccgcc accaccagcg
      721 agacccgcag ccaggcctgg aactcgggat cgcgcagcac cgtccagaag ttctgcggcc
      781 gccacgcgat cggcagcagg cgcggcgggg tgacccgcag ctcggcctcc ggcttgagcg
      841 cggtgagcag cattaccgcg tacggggcga ggagcgccgc cgccaccagc acccccaggc
      901 cccccagcgc cacccggcgg atcagaccgg ctcctcccgc caccgcgaga cccggaggta
      961 ggccagcacc gcgagcagga tcaggaccac gttgaccaca ccgagcgccg ccgcgaggcc
     1021 cacgtcccgc agcgcgctct tgaaggcgat cttgtacatg taggtgatca tcgtgtcgtg
     1081 cgccatcccg ggattgcggt cgttgagcgt ccacacgatc gggaaggagt tgaagacgta
     1141 gatgaggttc aggaccgcgg ccaccagcag ggccggccgg aggagcggca gggtgaccgc
     1201 gcgataggcc cgccagccgg cggcgccgtc cacccgcgcc gcctcgtaga cctcggccgg
     1261 gatggtcgac agcccggcga gcaggacgta agtggtgaac ggcagcgaga cgaagacccc
     1321 caccgcgatc atcgctccca tgacggtggc gtcgtcgccc agccagtcca ccggggcccg
     1381 cacgagaccg gcggccacga gggcgcgatt gatcaggccg aagtagtagt cgtagagcca
     1441 cgcgaagagc ttcgaggtca tgatgagcga ggcggcccac ggcacgatga gggcccagcg
     1501 cacgagccgg cgccccggaa acgtcgcgtc gaggagctgg gccagcccga gcgagatcac
     1561 gatcgtgatc ccgaccacca gggccaccca cgtcacggtg ttgccgatca cggtgggcag
     1621 cgcgtcctgc tcgaggaggc gcccgtagtt gcgcgcgccg accgccccct ggtagagccc
     1681 ggtgatcgag tagcgcccga gggacgcccg cacgagctga accgcggggt agaccaccac
     1741 cgccgcgatg aggaggagac tcgggccgag ccagaggagc gcccgcccca cgcccgcgcc
     1801 cgcggggcgg cggccgcgcg cgggcgggag acccgtcagc gaccgctctc cgccgcgttc
     1861 ttctggagcc ggtcgaggac ctgcttcgga tcgccgcccg gctgcacggc cagcccgatg
     1921 gtctgctgga cgtcgagctt caccttgtcc cacaccgggt cggtggtcgg ggcgaggtgc
     1981 gcgctcggca gcgcgtcgag gtacggcttg agcttctcgt tgccgctcat gcgctcccgc
     2041 ccggacttgg tgaccggcag gaagccctcc gcggtgatcc agcgcgtgat ctgctccggc
     2101 tggtagtaga ggtccaggaa ctgccgcacc gcctcctggt tgcccttctt cttgaaggcc
     2161 atcaggtagt cctcgacgcc gagggtggcc ggcgcgccga cattggtcgg catctgagtc
     2221 accccgtagc ggaccttacc ttccgcgtcg agctgggccg cgagcgggct gaagcccatc
     2281 accatcccga ccttgccgtc cttgaagagc tggaaggcgc cgtcggtgcg gttggtcttg
     2341 cccggattga cctgggtcac cttgtgggtg ttggcgagcc cggccaggaa ctggagcgtc
     2401 tggacattct tgtcgctgtt gatcgcccag gcatcgcccg acttccagtc gccgccgttg
     2461 ttccacatcc agatcgacca ctcggcctgc gcctcctcgg gaccgagcgg cagcgcgtag
     2521 ccgatcaccc cgccgccgag cgcctgcacc ttgcgcgcgg cctggaccag ctcgtcccac
     2581 gtgcgcggcg gcgcgttcac cttcgcgcgc tcgagcaggt ctcggttgta gaagaaggcg
     2641 cgcgcgctgg ccaggatcgg gaacccgtag agcttgtccc ggtaggtccc gccgcgcgcg
     2701 aaggcctcga ggaagtcgtc gcgcgtcttg ggggagagcg cctcgtccgc cgagtagagc
     2761 agcccgtcct tcgcgtagct cgagaacgag ttgagattga gcacgtcggg cggctggttg
     2821 ttctggatca tggtgctgac ctgctggtcg atggagttcc agtcgatcac ctggagatcc
     2881 accttgacgc cggtctgctt ggtgtattgc tcggcgaggc tctgccagaa gggccgggtg
     2941 tgatcgcgac tgtactccgc gatgaccacc ttgatcgtgg ccggcttgcc gtccccgcgc
     3001 ccgcacgcga cggtgacgac cgcggtcacg cagagcagcg ccaggaccag gaggcggcgg
     3061 ctcgtcatcg agcccatact acaaaagttc ccggcgaggg tgtaagtctt actcatgccc
     3121 tggacgccct cgggcgggcg tgattagatg acggaccgtt accggcggtc cccggcacgg
     3181 cacactactt gctgaattct caaggaggct ggtcccatgg cgcgagtctt gatcctcttc
     3241 acgctcgtcc tcaccctggc cgcgtgcgcc tcctcgtccg gccccgcgaa gccgcagcgg
     3301 agcgagttcg aggacgtccc ggtgcccgcg ggcctgaccc tggattcgaa ccggaccacc
     3361 atcatcgagt cgcccaccgt gaaggcggcg cggctgttct acaagagccg gatcaagccc
     3421 gacagcctct cagtcgccta ccggacctcg ctcgaggcca acgggtggcg ccacgtctcc
     3481 tccaccacct cggcgggcaa gggcacgacc caggtctacg agaagcagga cagctcgctg
     3541 caggtactca tctacgaggg ttggtactac acatggaccg aggtctcaac tacccggatc
     3601 accgctcgcc cggcccccgc caagtaggcg ctatcggatc gcggctgctg gcctgcgtcc
     3661 tgctcctcgg cgggtgcgcg gcgacccagt ccgcctacgc tccggtgccg gcgcgggacg
     3721 cgagcgtgga cgaccgcgtc accgccgtgg agggacggct cggcgagatg aaccagcgtc
     3781 tcgaccaggt tggcggccag gtccagcgcc tcgagggtgg tgtgaccacc gcccagggcc
     3841 gcgccgacga ggcggccaag aaggcggagg gcgtggacgg ccgcctcacc cggctctgga
     3901 cgaaccgctt caatcagaag caggccgaca gcctcgaggt ctacttctcg ccggacagca
     3961 tcgacctgac cgacgcggcc cagaccgcgc tcctcgcggt ggtccgcgag atggaggccc
     4021 accccacgct cacggtggag ctcggcggct acaccgatcc acgcggggcg ctcgactaca
     4081 actacggcct gagccagcgg cgggtggacg cggtgcggcg cttcctcatg gacaagggca
     4141 tccagctcgc ccgcatccag gccgcgagcc tgggcccgat cgccaccgcc ggcatcccgg
     4201 atgtgaagaa gcgccgcgtg accgtccgcc tgatggccga cccggagtag ggtcaggccc
     4261 cgtccttctg ggccggctgg taggtgccgg gcaccgcgcg gaagccgatg gcgagccggt
     4321 tccagggccc ggtaaacggt tcccgcgctc gctccgtcca atcgccatga ccacctaccg
     4381 gatgctggcc acctcggccg tcgcgctgct catggccggc tgcgtcgtcg cgcagccggt
     4441 ggtcgtgagg cggccgccgc ctcccccgcc cgtccgcgtg gagatcgcgc cggtggcacc
     4501 gaccccggcc cacgtgtgga tccccgggca ctggggctgg caccgcgagc actacgtgtg
     4561 ggtccccggc cactgggaag tcccggcctc ccccggctac gtttgggtgc cgggcgggtg
     4621 gacgccgcgc gagggcggct acgtctgggt cgaggggcac tggcgcgcgc gctgaaggcg
     4681 cggcgccctt gagtcggccc tacatcgggg ggacgagccc gtccttcccc acgagcacgc
     4741 gcgcggcggt cacggtgccg tcgggctgtt gctgggccgg gacgaacacg tgagcgcccg
     4801 gctggagcat cgccttgtcc ccgggcgcga acgtcacgat cggcacgtcg gtgggcaccg
     4861 ccaccgtgac gctgccaccc ttgtgcttga gcgtcaggat cggcccctct acccgctcga
     4921 ccatcgcgtc caccgtggcg ttggtcatgg tgctgccggg cgagagatcc cacgggt
//
LOCUS       DATMCV010000988         2851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_520325, whole genome shotgun sequence.
ACCESSION   DATMCV010000988 DATMCV010000000
VERSION     DATMCV010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520325"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VNU03_22610"
     CDS             complement(<1..253)
                     /locus_tag="VNU03_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWP77334.1"
                     /translation="MTHDPPLPTPFVERFAPLAEVKFSGPRSSFHRCFGCGPDHDIGL
                     RVRTFKAEGEVLSPIVIPGRFEGPPGAAHGGIVAAYLDEI"
     gene            complement(250..816)
                     /locus_tag="VNU03_22615"
     CDS             complement(250..816)
                     /locus_tag="VNU03_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWP77335.1"
                     /translation="MRPSAPMTGRLLEAMAHAGARRYGGERVSELDHALQCAELAEAG
                     GADEQLTLACLLHDVGRFVVDQSLVFDRMGGARSGARGHHEIGAELIAPYVPERVAWL
                     VRMHADAKRYLCSTEPGYHATLSPASQHTLTLQGGPMGAEEIACLDGHAWLADALRLR
                     RWDDGAKVAGRVTRPLESWMPLVRSYFG"
     gene            899..1087
                     /locus_tag="VNU03_22620"
     CDS             899..1087
                     /locus_tag="VNU03_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013171237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HWP77336.1"
                     /translation="MAVSVKVITDGPLMVKGECEMQDGQGNAMPPKGGDTIFLCRCGS
                     SANKPYCDGAHKKISFKG"
     gene            complement(1158..2309)
                     /locus_tag="VNU03_22625"
     CDS             complement(1158..2309)
                     /locus_tag="VNU03_22625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77337.1"
                     /translation="MSGDRFAGTALLCLVVFSSTVCIGAFGPLLPEIARAQGLPDWEL
                     GVLAGSFGLARMAADLPAGALATRRLATALTLAPVTLLAGMLLLATAGPLSVLVLGRI
                     LTGLAHTLVMVGSLTAVLQDQPGPRASMRLNTLEFAGMLGVLGGLVVVGLLPQRWAWN
                     VTLLAAATPVLVALAATLVLARYFPARLGAEAPAPGSAGATSATASGRPSLPPIVRLM
                     FALGVIMALAWSAVSQFVIPLRGTREFGLDRAGISGLLSLAQLVDLAVLLPVGWLADR
                     VGRPPVLVGVAAVLGVGLFAVGLGSLPLLGLGCALFGLGMAGWMLPLGVIRDHTDGAR
                     LGWRTGVYRVGVDGAIFLGPVVCGLLGEGRAPIFIGLIGVAAFAIAGRL"
     gene            complement(2323..>2851)
                     /locus_tag="VNU03_22630"
     CDS             complement(2323..>2851)
                     /locus_tag="VNU03_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP77338.1"
                     /translation="PAVGIGATMTLPMDIRLASENAKMGFVFARRGIVADGCASWFLP
                     RVVGISKALEWTLSGRVFGAAEALEAGLVRSLHPSDELLPAAMELAREIAENTSAISV
                     ALSRQLMWRLLGADHPMEAHRLDSRCIDWTGRSADAREGVSAFLEKRPARFTLRPSTD
                     MPPFYPWWRERSFEP"
BASE COUNT          464 a         1054 c         1034 g          299 t
ORIGIN      
        1 ggatctcatc gaggtaggcg gccacgatgc cgccgtgggc ggcgccgggc ggtccctcga
       61 agcggcccgg gatcacgatc ggcgagagga cctcgccttc cgccttgaag gtccggaccc
      121 ggaggccgat gtcgtggtcc gggccgcacc cgaagcagcg atgaaagctc gagcgggggc
      181 cgctgaactt cacctcggcg agcggcgcga accgctcgac gaacggcgtg ggcagcggcg
      241 gatcgtgggt catccgaagt agctccgcac cagcggcatc cacgactcga gcggccgggt
      301 cacccgcccc gccaccttgg ccccgtcgtc ccaccgccgg aggcgcaggg cgtcggcgag
      361 ccacgcgtgc ccgtcgaggc acgcgatctc ctccgccccc atgggcccac cctgcagcgt
      421 cagcgtgtgc tgcgaggcgg gcgagagggt cgcgtggtag ccgggctcgg tggagcagag
      481 ataccgcttg gcgtcggcgt gcatgcgcac gagccacgcc acgcgctccg gcacgtaggg
      541 agcgatcagc tcggcgccga tctcgtggtg gccgcgggcc ccggatcggg ccccgcccat
      601 gcggtcgaag accagcgact ggtcgaccac gaagcggccc acgtcgtgga gcaggcacgc
      661 gagcgtcagc tgttcgtccg cgccgccggc ctcggccagc tcggcgcact gcagcgcgtg
      721 gtccagctcg gacacgcgct cgcctccgta gcggcgggct cccgcgtgcg ccatggcttc
      781 gaggagacgg ccggtcatgg gggcgctcgg gcgcatgtcg atctggagta tactcgtcga
      841 gcggcagcca ctaccaccac ggtgatgcga acgcggtgat gcgaaaggga gagccacgat
      901 ggcggtgagc gtcaaggtga tcacggacgg gcccctgatg gtgaagggcg agtgcgagat
      961 gcaggacggc cagggcaacg ccatgccacc caagggcggg gacaccatct tcctctgccg
     1021 ctgcggcagc tcggcgaaca agccctactg cgacggcgcc cacaagaaga tcagtttcaa
     1081 gggctgaccc catcctctct ccctctcccc tccggggaga gggcagggtg agggggtgtg
     1141 gcctaccgcc gcatccatca cagccgcccc gcgatcgcga aggcggccac cccgatcagc
     1201 ccgatgaaga tcggcgcgcg cccctcgccc agcagaccgc acaccaccgg gccgaggaag
     1261 atcgcgccgt ccacccccac ccgatagacg ccggtccgcc agccgagccg cgccccgtcg
     1321 gtgtggtctc ggatcacgcc gagcggcagc atccagcccg ccatcccgag gccgaagagc
     1381 gcgcagccca gcccgagcag cggcagcgat cccagcccca ccgcgaagag gccgactccg
     1441 aggaccgccg ccaccccgac cagcaccgga ggccggccca cccgatcggc gagccagccc
     1501 accggcagca gcaccgccag gtccaccagc tgcgccagcg acagcaggcc ggagataccg
     1561 gcacgatcga gcccgaactc gcgcgtgccg cgcagcggga tgacgaactg gctcaccgcc
     1621 gaccaggcga gcgccatgat cacgccgagc gcgaacatca ggcgcacgat cggcggcagc
     1681 gacgggcgcc cggaggcggt cgcggacgtg gcacccgccg agcccggcgc cggcgcctcc
     1741 gcgccgagcc gggccgggaa gtagcgggcc agcacgagcg tggccgcgag cgccaccagc
     1801 accggcgtgg cggccgcgag cagcgtgacg ttccaggccc agcgctgggg caagagcccg
     1861 accacgacca ggccgccgag cacaccgagc atccccgcga actcgagcgt gttgagccgc
     1921 atcgaggccc gcgggccggg ctgatcctgg agcaccgcgg tgaggctgcc gaccatgacc
     1981 agggtgtggg cgaggccggt gaggatccgg cccagcacca gcaccgacag cgggcccgcg
     2041 gtggcgagca gcagcatccc ggcgagcagg gtcaccggcg cgagcgtcag cgcggtcgcc
     2101 agccggcgcg tggcgagcgc gcccgcgggc aggtcggcgg ccatgcgcgc gaggccgaag
     2161 gatccggcga ggacgcccag ctcccagtcg ggcagcccct gcgcacgggc gatctcgggc
     2221 aggagcgggc cgaaggcgcc gatgcagacc gtgctggaga agacgacgag gcacagcagg
     2281 gccgtgcccg cgaaccggtc tccgctcacc gcctggggcg gctcagggct cgaaggagcg
     2341 ctcgcgccac cacggataga agggcggcat gtcggtgctg gggcgcaggg tgaagcgggc
     2401 ggggcgcttc tcgaggaacg cgctcacgcc ctcgcgggcg tcggcggagc gtcccgtcca
     2461 gtctatgcac cgggagtcca gccgatgcgc ctccatcggg tggtccgcgc cgagcaggcg
     2521 ccacatgagc tggcgcgaca gggccaccga gatcgccgag gtgttctccg cgatctccct
     2581 ggccagctcc attgcggccg gcagcagctc gtccgagggg tgcaggctgc gcaccaggcc
     2641 ggcctccagc gcctccgccg cgccgaacac ccggccggac agcgtccact ccagcgcctt
     2701 cgagatgccg accacccggg gcaggaacca gctcgcgcag ccgtcggcca cgatcccgcg
     2761 ccgggcgaac acgaagccca tcttcgcgtt ctccgaggcc agccggatgt ccatgggcag
     2821 cgtcatggtc gcgccgatgc cgacggccgg a
//
LOCUS       DATMCV010000989         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_52029, whole genome shotgun sequence.
ACCESSION   DATMCV010000989 DATMCV010000000
VERSION     DATMCV010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52029"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VNU03_22635"
     CDS             <1..609
                     /locus_tag="VNU03_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWP77339.1"
                     /translation="RFVVDVASTAEQGDEMATATDYDLIVLDWLLPDQEGTALCREWR
                     ARGLAAPILMLTARDALEDRVTGLNTGADDYLVKPFGFSELIARIHALLRRSELTRPV
                     VLAVADLTLDPQSHRVARGGLPINLTPKEYAILEVLMRHAGRVVTRARLADRVWETEQ
                     DSLTNLVDVHVSHLRRKVDGSGGPPLIHTIRGRGYLVGPDAE"
     gene            606..2012
                     /locus_tag="VNU03_22640"
     CDS             606..2012
                     /locus_tag="VNU03_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77340.1"
                     /translation="MIALNLKARVFLLHVIAVGLILAAAAVGANWAFSRMVVGQFDTS
                     LLELAQTEAAAALANPSQPPHIHEMAPGAATPSFPRLDKLIQIVDLDGHVVARSANLG
                     SARLPTPPALIARLRQGEPVLETLPDFGDEPLRLLSVPLQLGTGAYVIQVGGSLDDVH
                     VATRWARWLFLTMSLAILGAVVLTGAMLARAILRPIDQIVLRARAMGASGLAERLPHP
                     GGRDEISRLVETLNEMLARVEHVFEAQRRFTADASHELRSPLSRLRAELEVTLRRPRE
                     RPEYEEALRSCLAEVARLSRLTEALLTLARLDAGESPKPPRQDVALAPILSETAARLE
                     ASGHDRDVSVVLEVPETLRVDVAPAAVSLVISNVLDNAIKFSPPGGKVTIEAFVEAGA
                     AVVAVSDTGPGVPEEELPRLFERFYRGSSARATDTPGVGLGLAICRLVVESQGGSIAL
                     VPAPGGGASIRVRLPLAP"
     gene            2066..>2633
                     /locus_tag="VNU03_22645"
     CDS             2066..>2633
                     /locus_tag="VNU03_22645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWP77341.1"
                     /translation="MPAEVIMGAAARWNLVARVIVVMVAAGGAFCLLAGPGVAQEPVR
                     RLSLDEALALALRQNPTVRAKVAELRSVQVSEITAGLRPNPTATYLAEQFGGGSAALV
                     QHTFSIGQPIELGGKRLRRVDAARAATRVTAFQLEDVRRLTVLQVKTAFAGALVAREQ
                     LALAEENLRALDETARLQRLRVDRGDLSE"
BASE COUNT          328 a         1002 c          936 g          367 t
ORIGIN      
        1 cgcttcgtgg tggacgtggc ctccaccgcc gagcaaggcg acgagatggc gaccgccacc
       61 gactacgatc tcatcgtgct cgactggctc ctgccggacc aggaggggac cgcgctctgt
      121 cgcgagtggc gggcgcgggg actggccgcg ccgatcctga tgctgacggc ccgcgacgcg
      181 ctcgaggacc gggtgaccgg cctcaacacc ggcgccgacg actacctcgt caagccgttc
      241 ggcttctccg agctgatcgc ccgcatccac gccctgctgc gccgctcgga gctgacccga
      301 ccggtcgtcc tcgcggtggc cgatctcacc ctcgacccgc agagccaccg cgtcgcgcgc
      361 ggcggcctcc cgatcaatct gacccccaag gagtacgcga tcctcgaggt cctgatgcgc
      421 cacgccgggc gggtggtcac ccgggcccgg ctcgccgatc gcgtctggga gaccgagcag
      481 gattcgctca ccaacctggt ggacgtgcac gtgagccacc tccgccgcaa ggtcgacggc
      541 tccggagggc cgcccctcat ccacacgatc cggggacgcg gctacctggt gggaccggac
      601 gcggaatgat cgcgctcaat ctcaaggccc gcgtcttcct gctgcacgtg atcgcggtgg
      661 gcctcatcct cgcggccgcg gcggtgggag ccaactgggc cttctcccgg atggtcgtgg
      721 gccagttcga cacctcgctc ctcgagctgg cccagaccga ggccgccgcc gccctcgcga
      781 acccgagtca gccgccccac atccacgaga tggcgccggg cgcggcgacg ccgtcgttcc
      841 cgcgcctgga caagctcatc cagatcgtcg acctcgacgg ccacgtcgtc gcgcgcagcg
      901 ccaatctggg ctcggcccgc ctgccgacgc cgccggcgct catcgcccgg ctccgccagg
      961 gcgagccggt gctcgagacg ctgcccgact tcggcgacga gccgctccgg ctgctctcgg
     1021 tgccgctcca gctcggaacg ggggcctacg tgatccaggt cggcggctcc ctcgacgacg
     1081 tccacgtcgc gacgcggtgg gcccggtggc tcttcctgac catgtcgctg gccatcctcg
     1141 gcgcggtcgt gctcaccggg gcgatgctgg cccgggccat cctgcggccg atcgaccaga
     1201 tcgtgctccg ggcccgggcc atgggggcca gcggtctcgc ggagcggctg ccgcaccccg
     1261 gcggccggga cgagatcagc cgcctggtgg agacgctcaa cgagatgctg gcccgtgtcg
     1321 agcacgtctt cgaagcccag cgccgcttca ccgcggacgc ctcgcacgag ctgcgctcgc
     1381 cgctctcgcg gctgcgggcc gagctcgagg tgaccctccg gcggccgcgg gagcggcccg
     1441 agtacgagga ggcgctgcgc tcgtgcctgg ccgaggtcgc gcgtctctcg cggctgactg
     1501 aggcgctgct gacgctcgcc cgcctggacg cgggggagtc gccaaagcct ccgcgccagg
     1561 acgtggcgct cgcgccaatc ctgagcgaga ccgccgcccg gctcgaggcc agcggccacg
     1621 accgcgacgt gagcgtggtc ctcgaggtcc cggagacgct ccgggtcgac gtggcgcccg
     1681 cggcggtgag cctcgtgatc agcaacgtgc tcgacaacgc gatcaagttc tcccctcccg
     1741 gcggcaaggt cacgatcgag gcgttcgtcg aggctggcgc cgcggtggtg gcggtctcgg
     1801 ataccggccc cggagtgccc gaggaggagc tgccgcggct cttcgagcgc ttctaccggg
     1861 gcagctcggc ccgggccacc gacacgcccg gcgtcggcct ggggctcgcg atctgccgcc
     1921 tcgtggtgga gtcgcagggt ggcagcatcg ccctggtgcc ggcgcccggc ggcggcgcga
     1981 gcatccgcgt ccgcctcccg ctcgcgccgt aaccgccccg tcggcgcggc cgccttcagg
     2041 attccttcat ccgcgccggg ccactatgcc cgccgaggtg atcatgggcg cggcggcgcg
     2101 atggaatctg gtggcgaggg tgatcgtggt gatggtggcg gcgggtggtg cgttctgcct
     2161 cctcgccggc ccgggcgtcg cgcaggagcc ggtccggcgg ctgtctctcg acgaggcgct
     2221 cgccctcgcg ctgcgccaga acccgacggt gcgcgccaag gtcgcggagc tccggtcggt
     2281 gcaagtcagc gagatcaccg ccgggctgcg accgaacccg accgcgacct acctcgccga
     2341 gcagttcggc ggcggcagcg cggccctggt ccagcacacc ttctcgatcg gccagcccat
     2401 cgagctcggt ggcaagcgcc tccggcgcgt cgacgcggcc cgcgccgcga cgcgggtcac
     2461 cgccttccag ctcgaggacg tgcggcggct caccgtcctc caggtcaaga ccgccttcgc
     2521 gggcgccctg gtggcgcgcg agcagctcgc gctcgcggag gagaatctgc gggccctcga
     2581 cgagaccgcc cgactgcagc gcctgcgggt cgaccgcggc gacctgtccg agc
//
LOCUS       DATMCV010000990         2416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_718579, whole genome shotgun sequence.
ACCESSION   DATMCV010000990 DATMCV010000000
VERSION     DATMCV010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718579"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="VNU03_22650"
     CDS             complement(<1..1571)
                     /locus_tag="VNU03_22650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77342.1"
                     /translation="MTRFLRSLMILGGFTLTLALAALAPAAAQEQPRAGGELIFVVAA
                     EPPSFDGHREETFAMLHPTGPHYSTLMRVDPTDRTGTKFIGDIAESWTISPDKRTYTF
                     KIRRGVKFHDGALLTSADVKASYDHIIFPPAGVVSSRQAAYRGVESVEAPNADTVVFR
                     LKAPEASFMANVSSPWNYIYRAEILAKDPRWYEKNINGTGPYKFVEYVRGSHWVGKKN
                     PDYWDKGKPYLDGYRAIFIQDASAQVAAIRGERAMIQFRGFTPAQRDGLVQALGNKIT
                     VQESPWNCSIQVAMNQEKKPFGDKRVRRALSLALDRYAGSKALSRIAVVKDVAGIQVP
                     GTPWATPPEELQKLAGYGTDINASRAEARRLLKEAGAEGLSFTLTNRGVPQPYEPVGI
                     WLIDQWRQVGLNVKQVTLESAAWLQAQKNGDFEVSTNAPCNSIVEPDMDLHWFLTTSP
                     VNFSRHKDTVMDDLYQRQSRSTDPDERKKILRAFEKRLYDEEVHYIMTFQWNRIVPYN
                     SRVRGWTITPSHFLN"
     gene            1661..>2416
                     /locus_tag="VNU03_22655"
     CDS             1661..>2416
                     /locus_tag="VNU03_22655"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77343.1"
                     /translation="MTRALRPLVPLLPLMALALLGPAPAGAQPAGTVTIAAHITLATR
                     WLDPAETDAEITPFMILYALHDALVKPMPAALNAPSLAESWSMSKDGRTWEFVLRKGA
                     RFHNGDPVTADDVKFSFERYRGAAAPILKDRVRDVQVVDPGRVRFHLKDPWPDFMTFY
                     GTSASGAAWIVPKKYLEKVGEDGFRRAPIGAGPYKFVSFQPGIELTLEAFDGYWRKVP
                     SVKRLVIRSIPEESTRAAALKRGEVDIAYFLNGP"
BASE COUNT          353 a          804 c          852 g          407 t
ORIGIN      
        1 cagttcaaga aatggctcgg ggtgatggtc cagccgcgca cccgggagtt gtagggcacg
       61 atgcgattcc actggaaggt catgatgtag tgcacttcct cgtcgtagag gcgcttctcg
      121 aaggcgcgca ggatcttctt gcgctcgtcc gggtcggtgg agcgcgactg gcgctggtag
      181 agatcgtcca tcaccgtgtc cttgtggcgg ctgaagttga ccggtgacgt ggtcaggaac
      241 cagtgcaggt ccatgtcggg ctcgacgatg gagttgcacg gggcgttggt ggagacctcg
      301 aagtcgccgt tcttctgcgc ctgcagccac gcggcggact cgagggtcac ctgcttgacg
      361 ttgaggccga cctgccgcca ctggtcgatg agccagatgc ccaccggctc gtagggctgc
      421 gggacgccgc ggttggtcag ggtgaatgag agcccctccg cgcccgcctc cttcaggagg
      481 cgccgcgcct cggcgcggga cgcgttgatg tcggtgccgt agccggccag cttctgcagc
      541 tcctccggcg gggtggccca cggcgtgccc ggcacctgga tgccggcgac gtccttcacc
      601 accgcgatgc gcgacagggc cttcgagccg gcgtagcggt ccagcgcgag cgagagggcg
      661 cggcgcaccc gcttgtcgcc gaaaggcttc ttctcctggt tcatcgccac ctggatcgag
      721 cagttccagg ggctctcctg caccgtgatc ttgttgccga gcgcctggac caggccgtcc
      781 cgctgcgcgg gcgtgaagcc gcggaactgg atcatggccc gctcgccgcg gatggccgcc
      841 acctgcgcgc tcgcgtcctg gatgaagatg gcgcggtagc cgtcgaggta gggcttgccc
      901 ttgtcccagt agtccgggtt cttcttgccg acccagtgcg agccgcgcac gtactccacg
      961 aacttgtagg ggccggtgcc gttgatgttc ttctcgtacc agcgcgggtc cttggccagg
     1021 atctccgccc ggtagatgta gttccacggc gaggagacgt tggccatgaa cgaggcctcg
     1081 ggcgccttca gccggaacac caccgtgtcc gcgttcgggg cctccaccga ctcgacgccg
     1141 cggtaggcgg cctgccggct cgagaccacc ccggcgggcg ggaagatgat gtggtcgtag
     1201 gaggccttca cgtcggcgga ggtgagcagg gcgccgtcgt ggaacttcac cccgcggcgg
     1261 atcttgaaag tgtaggtgcg cttgtccggg gagatcgtcc aggactcggc gatgtcgccg
     1321 atgaacttcg tgccggtgcg gtcggtcggg tccacccgca tgagcgtgct gtagtgcggg
     1381 ccggtcggat ggagcatcgc gaaggtctcc tcgcgatggc cgtcgaagct gggcggctcg
     1441 gcggccacca cgaagatcag ctcgcccccc gcgcggggct gctcctgggc ggcggccggg
     1501 gccagggccg cgagcgcgag cgtgagggtg aagccgccga ggatcatcag tgatcgcagg
     1561 aaccgggtca tgtcgaaacc tccgagcgcg agtctaccag aaggcgcccg tggtagtctc
     1621 tggccgtccc accctcggtg agagatggca ggaggcgccc atgacccgtg cgctccgacc
     1681 gctcgtcccg ctcctcccac tcatggcgct cgcgctgctc ggcccggcgc ccgccggtgc
     1741 ccagcccgcc ggcaccgtca ccatcgcggc ccacatcacg ctggcgacgc gatggctcga
     1801 cccggcggag accgacgccg agatcacgcc cttcatgatc ctgtacgcgc ttcacgacgc
     1861 cctcgtgaag ccgatgcccg ccgccctcaa cgccccgagc ctggccgagt cgtggtccat
     1921 gtcgaaggac gggcggacct gggagttcgt gctccgcaag ggggcgcgct tccacaacgg
     1981 cgacccggtc accgccgacg acgtgaagtt ctcgttcgag cgctaccgtg gcgcggccgc
     2041 gcccatcctc aaggatcgcg tgcgcgacgt gcaggtcgtg gatccgggcc gcgtgcggtt
     2101 ccacctgaaa gatccgtggc ccgacttcat gaccttctac ggcacgagcg cgtccggcgc
     2161 cgcctggatc gtgccgaaga agtacctcga gaaggtgggc gaggacggct tccgccgggc
     2221 gccgatcggg gccgggccgt acaagttcgt gagcttccag cccggcatcg agctgaccct
     2281 cgaagcgttc gacggctact ggcgaaaggt tccgagcgtg aagcggctcg tcatccgcag
     2341 tattccggag gagtcgaccc gcgcggcggc gctcaagcgc ggcgaggtgg acatcgccta
     2401 cttcctgaac gggccg
//
LOCUS       DATMCV010000991         5166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_187404, whole genome shotgun sequence.
ACCESSION   DATMCV010000991 DATMCV010000000
VERSION     DATMCV010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187404"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VNU03_22660"
     CDS             complement(<1..1503)
                     /locus_tag="VNU03_22660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77344.1"
                     /translation="MTVVTLALAYGVWYAYSVFLVALLREFGWSRSVLAGAFSVFTLV
                     SGGAGPVLGALADRFGPRRLILIGGVLLAASLWADSFVTRAWHLYLAFGLMTAIGVAT
                     AGWTPAVVLVQRQWHARLGLALGIAGSGVGLGIFLVVPLCQGLIDAFGWRWAFRGLGT
                     LAFLWILPATALFVHDVPAAPRPAAASPGEIAGAGAFSLEKALASGSFRFIALAVFLG
                     SICSQTLHVHQAAFFVDHGLPAMTAASVISVVGAASILGKTGGGFVSDLFPREAVYAL
                     GMVGMIGGVGVLWMVAAAPSAWLALGYGVLFGLGYSVTAFVVPAMMSDRFPGAELRGD
                     LRGDPGGERARQCAGGVAGRADLRRDGQLRARVLDRGRGGGGGRAVGVGRPHAAAGGR
                     LGALAEERGRAAGGAGGTFEEAGMLARDRGPGPRGRGPAKPRGRRDLGGVRANRDGDV
                     FFSWCGRRRGSRRHRGRGFRGRDRGQIRGTGGIEEMRQVRDRHEGDQADRG"
     gene            complement(1581..2918)
                     /locus_tag="VNU03_22665"
     CDS             complement(1581..2918)
                     /locus_tag="VNU03_22665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP77345.1"
                     /translation="MIGSFVFVAAVLLAGCSGPDARVRGPLQSGDDAARQHALREALN
                     HYRTAAVAEPASLEAQTRRGAMAEALGEFDEALEAYGRAARLEPSALTFYRAGAMAER
                     MGNHGLAVDYLTASLSAAPTRPERVAQAGRHGIERLSAMLAGSRFSRMLPDWVFRSLT
                     MGQVVLSNAGLDRDVVAGTLLASQIEAGETTRAFDLARARGWVRAGADYCAGARGDAA
                     ETRALVGMLAAPEQTDCLLAVGRSLTDGGMVRLARLVLLDRSRHSADPRVRRDALAFL
                     RERLPRDVSKVAESLNVAAYNLHHRFRDQGDAAVVYQRAIAADPEFSWPYANLGRLYM
                     DMDEAELGLEWLEAATRLDPNHFRAWVNLGLALYSLRRYEEAVPAYQAALAINPNDAF
                     VHAYLGRSLLSLGRDGEGVRELQTAVRLDPSLTEERDLLGRRVAADPRAFQPD"
     gene            complement(3099..3839)
                     /locus_tag="VNU03_22670"
     CDS             complement(3099..3839)
                     /locus_tag="VNU03_22670"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP77346.1"
                     /translation="MMGLRMRQICLVARSLAPVVEEIRSVLGLEVCHRDPGVGKFGLE
                     NALFPVGDGYLEVVAPVRDGTTAGRYLDRRGGDGGYMVITQCGGDDLAARRARCETVG
                     VRIANEIGYPEYRELQLHPRDVGAAMLSFSWQEGARAPGGPWHPAAYDGTRSPARAGV
                     IRAMTAAELQSEDPERLAQRWSQVIERPVVLREGQRTIALDDATLRFTEASDGRGEGL
                     AGLDLDCADPARRGLPLICGIRFRLVAP"
     gene            complement(3836..5065)
                     /locus_tag="VNU03_22675"
     CDS             complement(3836..5065)
                     /locus_tag="VNU03_22675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77347.1"
                     /translation="MKFGIFYEHQLPRPWGADSEYRLLQDSLTQIELADRLGYDYAWE
                     VEHHFLEEYSHSSAPEVFLGAATQRTSRIRLGHGIIQLPTNHPIRVAERVSTLDLLSG
                     GRVELGLGEGQGPIELHPFGRRVRDKREVWEEAVRVLVPCFTQDAVEYHGQFFDFPAR
                     NVIPKPRQKPHPPLWVACSNIATIASAGQWGMGALGFQFVSPEAARAWVNRYYTSLTR
                     DLKRLADYPVNANIAMVSAFMCAPTDEEAREKAAGWTFFVFCLSHYGRHGMAEPGRGD
                     MWKLYQEWRHTPKAQETLKAGLIGSPETIRRRLAEFEATNVDQVILLNQTGRTSHQDI
                     CESLELFAREVMPEFHAREPEHQRWKDDVLAGRIELEQLDTEGHKLYSHQNEDIVRLT
                     PEELKRKMAAKEAERPA"
BASE COUNT          760 a         1905 c         1858 g          643 t
ORIGIN      
        1 gcctcggtcg gcctggtcgc cctcgtgtcg gtcccgtacc tgtcgcatct cctcgatccc
       61 tccggtgccc cggatctgac cccggtcgcg gccgcggaac ccccgcccgc ggtggcgtcg
      121 cgacccgcgc cgccggccgc accacgagaa gaatacatcg ccatcccggt tcgcgcggac
      181 gccgccgagg tcccgacgcc cacgcggctt cgccggtccc cgcccgcgcg ggcccgggcc
      241 gcggtcgcgc gccagcattc ccgcttcctc gaaggtcccg ccggcgccac cggcggcgcg
      301 gccccgctcc tcggcgagag cccctagccg ccctccggcc gcggcatgcg gccgccccac
      361 accgacagcg cggccaccgc cgccgccccg gccgcgatcg agaacgcgag cgcgtagctg
      421 cccgtcgcgt cgaagatccg cccggccagc cacgccccca gcgcactgcc gagcgcgctc
      481 gccacctggg tcgccccgaa gatcgccccg aagttcggcc cccgggaacc ggtcgctcat
      541 catggccggc accacgaagg cggtgaccga gtagccgagc ccgaacagca cgccatagcc
      601 cagggcgagc cacgccgacg gcgcggccgc caccatccac aacacgccga cgcccccgat
      661 catccccacc atcccgagcg cgtagaccgc ctcacgcggg aacagatccg agacgaagcc
      721 gccgccggtc ttgcccagga tgctggccgc gcccaccacg ctgatcaccg aggccgcggt
      781 catggcggga agaccgtgat cgacgaagaa ggcagcctgg tgcacgtgca gcgtctggct
      841 gcagatgctg ccgaggaaca ccgcgagcgc gatgaagcgg aacgacccgc tcgcgagcgc
      901 cttctcgagc gagaaagcgc ccgcccccgc gatctcgccc ggcgaggccg cggccggtcg
      961 cggcgccgcc gggacgtcgt ggacgaacag cgcggtggcg ggcaggatcc agaggaacgc
     1021 gagcgtgccg agcccgcgga aggcccagcg ccagccgaaa gcgtcgatga gcccctggca
     1081 gagcgggacg acgaggaaga tgcccaggcc cacgcccgaa cccgcgatgc cgagcgccag
     1141 cccgagccgc gcgtgccact gtcgctgcac caggaccacc gcgggcgtcc atcccgcggt
     1201 ggccaccccg atcgcggtca tgaggccgaa ggccaggtag aggtgccacg cccgcgtgac
     1261 gaagctgtcg gcccagagcg aggccgcgag cagcacgccg ccgatcagga tgaggcggcg
     1321 cgggccgaac cgatccgcga gcgcgcccag caccgggccg gcgccgccgc tcacgagagt
     1381 gaagaccgag aacgcgcccg cgagcaccga gcggctccag ccgaactcac ggaggagcgc
     1441 gacgaggaag acgctgtacg cgtaccagac cccgtaggcc agcgcgaggg tgacgacggt
     1501 cacggcgagg gcgcggtacg ccgggaggcg gggggcgacc gcgggatcgg ggcgcatggg
     1561 cgccccacca taccgcgagg tcagtccggt tggaaggcgc gcgggtcggc ggccacgcgc
     1621 cggccgagga ggtcccgctc ctcggtcagg ctcgggtcga gccggaccgc ggtctgcagc
     1681 tcgcgcacgc cctcgccgtc gcggccgagg ctcagcaggc tgcgcccgag gtacgcgtgg
     1741 acgaaggcgt cgttcggatt gatggcgagc gcggcctggt aggcgggcac cgcctcctcg
     1801 tagcgccgca gcgaatagag ggcgaggccc aggttcaccc aggcgcggaa gtggttgggg
     1861 tcgagccggg tcgcggcctc cagccactcg agcccgagct cggcctcgtc catgtccatg
     1921 tagagccggc cgaggttggc gtagggccag gagaactccg ggtcggccgc gatggcccgc
     1981 tggtagacga ccgccgcatc gccctgatcg cggaagcgat gatggaggtt gtaggccgcg
     2041 acgttgaggg actccgcgac cttggacacg tcgcggggca ggcgctcgcg caggaaggcc
     2101 agcgcatcgc ggcgcacccg cgggtcggcg gagtggcgac tgcgatccag caacacgagc
     2161 cgcgccaggc ggaccatgcc gccgtcggtc aggctgcggc cgaccgccag gaggcaatcg
     2221 gtctgctccg gcgcggccag catcccgacc agcgcgcgcg tctcggccgc atcgccacgg
     2281 gcgcccgcgc agtagtcggc gccggcccgg acccagccgc gggcccgcgc caggtcgaac
     2341 gcgcgggtcg tctcgccggc ctcgatctgg gaggcgagga gcgtgcccgc caccacgtca
     2401 cggtcgagcc cggcattgct caggaccacc tggcccatgg tgagcgagcg gaagacccag
     2461 tcgggcagca tgcgggagaa ccgcgagccg gcgagcatcg cggagaggcg ctcgatgccg
     2521 tgccggccgg cctgggccac ccgctcgggg cgggtcggcg ctgcgctcag gctcgcggtc
     2581 aggtagtcca cggcgagccc gtggttaccc atgcgctcgg ccatcgcccc ggcgcggtag
     2641 aaggtgagcg cggagggctc gaggcgggcc gcccggccat acgcctcgag cgcctcgtcg
     2701 aactcgccga gcgcctcggc catcgcgcca cgacgcgtct gggcctcgag cgaggccggc
     2761 tccgccaccg ccgccgtccg gtagtggttc agcgcctcgc gcagcgcgtg ctggcgggcc
     2821 gcgtcgtctc ccgactggag cgggccccgc acccgcgcgt ccggcccgct gcagcccgcg
     2881 agcaggacag cggccacgaa gacgaaacta ccgatcaacc acgcaaggtg tcgtccgggg
     2941 cgcaccatcg cgccttccaa gcggcaacat tcttgccact ccggtgacgc ggctaagtgc
     3001 gtggatttgc ggcggcgagt accgagtgtc gacgggtgat catccgtcgg ctgacagacc
     3061 taccgtccgg tgtcgaccgg acaccggtcg gaggccgcct acggggcgac caggcggaag
     3121 cggatgccgc agatgagtgg gaggccgcga cgcgccggat ccgcgcagtc gaggtcgagg
     3181 ccggcgagcc cctcaccgcg cccgtcgctg gcctcggtga agcgaagcgt cgcgtcatcg
     3241 agggcgatcg tgcgctggcc ctctcgcaag accaccgggc gctcgatcac ctggctccag
     3301 cgttgcgcga ggcgctccgg gtcctcgctc tgcagctcgg ccgcggtcat ggcccggatg
     3361 acgccggccc gcgcggggga gcgagtcccg tcgtacgcgg ccggatgcca gggaccgccg
     3421 ggggcgcggg ccccctcctg ccaggagaac gagagcatgg ccgcgccgac gtcgcgcggg
     3481 tgcagctgca gctcccggta ctccggatag ccgatctcgt tcgcgatgcg aacccccacc
     3541 gtctcgcacc gggcccggcg ggcggccagg tcgtcgccgc cacactgcgt gatcaccatg
     3601 taacccccgt cgcctccgcg gcgatcgagg taccggccgg cggtggtgcc gtcgcgcacc
     3661 ggcgcgacga cctccagata gccgtcgccc accgggaaca gcgcgttctc gagcccgaac
     3721 ttgccgaccc cggggtcgcg gtggcacacc tcgagcccca gcaccgagcg gatctcctcc
     3781 accacggggg ccaggctccg cgcgacgagg cagatctgcc gcatccggag gcccatcatg
     3841 ccgggcgctc cgcttccttg gccgccatct tgcgcttcag ctcctcgggg gtcagccgca
     3901 cgatgtcctc gttctggtgg gaatagagct tgtggccctc ggtgtcgagc tgctcgagct
     3961 cgatccggcc cgcgaggacg tcgtccttcc agcgctggtg ctcgggctcg cgggcgtgga
     4021 actcgggcat gacctcccgg gcgaacagct cgaggctctc gcagatgtcc tggtggctgg
     4081 tgcggccggt ctggttgagc aggatgacct ggtccacgtt ggtcgcctcg aactcggcga
     4141 gcctccgccg gatggtctcg ggcgacccga tgaggccggc cttgagggtc tcctgggcct
     4201 tcggggtgtg gcgccactcc tggtagagct tccacatgtc gccccgcccg ggctcggcca
     4261 tgccgtgccg gccgtagtgc gagaggcaga acacgaagaa ggtccagccc gccgccttct
     4321 cgcgcgcctc ctcgtcggtg ggggcgcaca tgaaggcgct caccatcgcg atgttggcgt
     4381 tgaccgggta gtcggcgagg cgcttgagat cgcgggtgag actcgtgtag tagcggttca
     4441 cccaggcgcg cgccgcctcc ggtgagacga actggaagcc gagcgcgccc atgccccact
     4501 gccccgcgct ggcgatggtg gcgatgttcg agcacgcgac ccagagcggc gggtgcggct
     4561 tctgccgggg cttggggatg acgttgcggg ccgggaagtc gaagaactgg ccgtggtact
     4621 cgaccgcgtc ctgggtgaag cacggcacca gcacgcgcac cgcctcttcc cagacctcgc
     4681 gcttgtcccg cacccggcgc ccgaagggat gcaactcgat cggcccctgc ccttcgccca
     4741 accccagctc gacgcgaccg ccggagagca gatcgagcgt ggacacgcgc tcggccaccc
     4801 ggatcgggtg attggtcggg agctggatga tcccgtggcc gagccggatg cggctcgtgc
     4861 gctgggtggc cgcgccgagg aacacctccg gcgcggacga gtgcgagtac tcctcgagga
     4921 agtggtgctc cacctcccac gcgtagtcgt agcccaggcg gtcggccagc tcgatctggg
     4981 tgagcgagtc ctggagcagg cggtactcgc tgtccgcgcc ccacggccgc ggcagctgat
     5041 gctcgtagaa gatgccgaac ttcatgaggt cgcctccgtt cagcgaagct cgtcgacgtg
     5101 gcgcacacgc cctgcgtcgc gggtgaagag gacgtcgttg tagccttgac ccgcgccgac
     5161 catctc
//
LOCUS       DATMCV010000992         7752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_394288, whole genome shotgun sequence.
ACCESSION   DATMCV010000992 DATMCV010000000
VERSION     DATMCV010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394288"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VNU03_22680"
     CDS             <1..270
                     /locus_tag="VNU03_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWP77348.1"
                     /translation="GLFISLAGPVTYKNARALPEVARFVPADRLVLETDCPFLPPHPH
                     RGQRNEPAWVAITAAHVATLRGVTLETLGPIVTANARRLFRLSDR"
     gene            295..1074
                     /locus_tag="VNU03_22685"
     CDS             295..1074
                     /locus_tag="VNU03_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP77349.1"
                     /translation="MAYTDILFEVKSQIAWLTINRPDKGNTFRRQTVLEMTQALNEAR
                     ADAGVRVVVITGTGDRFFCIGGEKEPNDGQLHYHNVPPVVDLYTLIDMMPVPVIAMVN
                     GFAVGGGNVLANMCDLTVASEQATFRQVGPAMGSYDAGFGTWYLEDSVGRKRAKEIWY
                     LNRKYSAKEAQAMGLVNEVAPHAELRARTEALCEELKKRGPQALAALKAAFHARHNGV
                     TGLSRLAVDHLVANYYHTEEAKEMGRAFNSKQDPDPDKFYR"
     gene            complement(1134..1460)
                     /locus_tag="VNU03_22690"
     CDS             complement(1134..1460)
                     /locus_tag="VNU03_22690"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HWP77350.1"
                     /translation="MPARRAKIIPFPVRPAARAGAPAGGPPTGRPPESSLPAGTLVEA
                     RRCRDQAEALVVRGLLESEGIRVVLQSRLAQSVYPFSVGDQAEVVLLVAAADLPRART
                     VLARER"
     gene            complement(1466..2656)
                     /locus_tag="VNU03_22695"
     CDS             complement(1466..2656)
                     /locus_tag="VNU03_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HWP77351.1"
                     /translation="MSEVRVEFLLTAVVGLGALSIDMFLPSLPAIAVAFAAPPATVQL
                     TVTLFLMGFAAAQLVYGPLSDRYGRRWVLVGGLGLYTAAGLACTLAPGVGVLIAARVL
                     QALGGGAGPVVARAVVRDLYDRERAARVFSYMSMAQSLNPMLAPIAGGYLHEAFGWRA
                     IFWLLTALGALFVVLMVVGVPETNVRRDPTALQPGQMGRNLAVLLGDRSYLGYVLVNA
                     LMFGGQFAFISGSSFVLIGVLGVSPSTFGLCFGTVALGIMTGTFLSGRFGGRIGLDRT
                     ILVGTTLGAGAGLLLAALAWSGVLTVAAVVAPMYVFAVGLGLTLPNGMAGAIGPFPRM
                     AGLAAAVAGFLQLTGSALYSMAVGHFFDGTARPMATAIALAGVAALACFAGLRSRRPP
                     DVLA"
     gene            2721..3968
                     /locus_tag="VNU03_22700"
     CDS             2721..3968
                     /locus_tag="VNU03_22700"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77352.1"
                     /translation="MRAFAIRMGAILLGAVSLLVGAGAAPAAAQGPIKIGFLSPLSGA
                     IAQAGKDMYSGCELYWQEHGMQIAGRKLQVILEDTEGQPATAVAKLRKLIDSDRVNLL
                     AGVILSNVAYALVPPIEAAGIPTIYPINSADDLTQRKRPKWLVRTGFSAGGNMHPFGE
                     YAAKTLKYKKVVTIGLDYAFGWETVGGFHKSFEDNGGQVIQKIWVPLNVQDYAPYLAQ
                     VKRDADAVFVVALGRWSVLFSKQYADSGLKGRIPLIAGGTYNDEHVLSQLGDEAIGVI
                     SAHHYSAALDTPANRTFRAAFEKAYSRPPSFYSENCYTGARVIEEAVKAVGGRVEDRA
                     ALMAALRRVEIKDAPRGPVKIDEYGNPTQNIYIRKVEKVGGVLQNTVIYTYPAVSQFW
                     TYDPQKFLEQPVYSREFPPCRHC"
     gene            4050..5099
                     /locus_tag="VNU03_22705"
     CDS             4050..5099
                     /locus_tag="VNU03_22705"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP77353.1"
                     /translation="MNPGLSTRDAQLPAAYSARLDALAVAPLWTVLHSLLPHERTTRV
                     LPHHWRWRELREPLLEAARLVPIEQAERRVLMLCNPGLPGNASATATLYAGFQIILPG
                     EAAPSHHHTAAALRLIVEGEGAYTTVDGVKCVMERGDLIITPPMRWHDHGHEGRDPVI
                     WLDGLDIPIVRSVEANWASAMKPASTPSSDVDSSQDEFTAAGLVPRRSRYEETGYPQV
                     RWPWRQVRAALAALAATAPRRTPVVLRHVNPRTGEYPLRTMGSETRWLRPGEDVRPER
                     RTGSEVVYVVEGRGESRIGDRVIAWEEGDVLAVPPWHWVEHQSRSAGGPACLFHLNDE
                     PALRALGLWHEEAGA"
     gene            5124..5846
                     /locus_tag="VNU03_22710"
     CDS             5124..5846
                     /locus_tag="VNU03_22710"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWP77354.1"
                     /translation="MEARYEALMDVIRGRMTNRAFAPAEIPRAHVEMILEAARHAPSG
                     ANAQPWHYVVVTNPDVKQAIGQSFVDEQQRRARLRMGFPTPNYNGVKTAPGLVVVVAD
                     FRFVRAFPVLNDGSETDRRYRQNAERILLQSVAASTMSAHLAAAALGYAVWWITAIGQ
                     DDLQQTIRPLVGVPEFLSIIDVMCFGPPLKPSYKRWKKRLDQIVSWDRMDPDDVMTDE
                     QIDEWVKTTRHKVMYRDEARVD"
     gene            5846..6787
                     /locus_tag="VNU03_22715"
     CDS             5846..6787
                     /locus_tag="VNU03_22715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP77355.1"
                     /translation="MLSVTEVDRVSVTTVVDNYVDVLRQDEKVARRFSAFSARQMPDL
                     RAEHGLAHHVEVARGRETVRIAFDFGSSETALTHNFRALGLDASAIDVLALSHGHWDH
                     WGGLAGMLRTYRRRMKRDLTFYGGEDHFLARFNQRGADRVNMGRLHRDEIERYDIRIE
                     AVRAPLMIADGVLLSGEMREQEPFEPIPDNLKVERDGAIVPDSFMGEQTLIANLKGRG
                     LVIVTSCSHRGIVGICRHAARITGVPKVHAVIGGFHLSGLKEERVTRVVDAFRDLEVD
                     WIVPQHCTGLEAMASMMHRLPQEMVPSSVGSVFTFGK"
     gene            complement(6791..>7752)
                     /locus_tag="VNU03_22720"
     CDS             complement(6791..>7752)
                     /locus_tag="VNU03_22720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HWP77356.1"
                     /translation="PVAAHALMIPGLRVGHVTDAAVLTGCTVILSDAPAIGGVEIRGW
                     AAGVHGLEFLDPRHLVPTLDGVVLAGGSAFGLEAIWGVMQYLEERGVGFRTSQTVVPH
                     VAGAILYDLGVGDARVRPDRAMGYRAAAAARPGPVEEGNVGAGTGATVGKIGGVARAT
                     KGGLGCAVGELDGVHLGAIMAVNAVGDVRDPETGRLIAGARDAADGRRLIDTAAALAA
                     GVPAPAFRPVNTTIGLVATTAALDKVEAGRVARLAMDGFTRALSPPHLATDGDTLFCL
                     SVGTGDAPVEALGRALADLVARAIVRGVRAATGAAGLPAARDL"
BASE COUNT         1207 a         2822 c         2735 g          988 t
ORIGIN      
        1 gggctcttca tctcgctcgc cggcccggtg acctacaaga acgcgcgcgc gctgcccgag
       61 gtcgcccgct tcgtccccgc ggaccgcctc gtgctggaga ccgactgccc gttcctgcct
      121 ccgcatccgc accgcggcca gcgcaacgag cccgcgtggg tcgccatcac cgcggcccac
      181 gtggccaccc tgcgcggggt gaccctcgag acgctgggtc cgatcgtcac cgccaacgcg
      241 cgccgtctct tccgcctgag cgaccgctaa cccatcgagc aaggagtcgc cctcatggcc
      301 tacaccgaca tcctcttcga ggtgaagagc cagatcgcct ggctcacgat caaccggccc
      361 gacaagggca acaccttccg gcgccagacc gtgctcgaga tgacccaggc gctcaacgag
      421 gcgcgcgccg acgcgggggt gcgggtcgtg gtgatcaccg ggaccggcga ccgcttcttc
      481 tgcatcgggg gcgagaagga gcccaacgac ggccagctcc actaccacaa cgtgccgccg
      541 gtggtcgatc tctacaccct gatcgacatg atgccggtgc cggtcatcgc gatggtcaac
      601 ggcttcgcgg tgggcggcgg caacgtgctc gccaacatgt gcgacctcac ggtggcgagc
      661 gagcaggcga cgtttcgcca ggtgggcccc gcgatgggct cctacgacgc cggcttcggc
      721 acgtggtatc tcgaggactc ggtgggccgc aagcgggcca aggagatctg gtacctcaac
      781 cgcaagtatt ccgcgaagga ggcgcaggcg atgggcctcg tcaacgaggt cgcgccgcac
      841 gcggagctgc gcgcgcgcac cgaggcgctg tgcgaggagc tgaagaagcg ggggccgcag
      901 gccctggccg cgctcaaggc cgccttccac gcgcggcaca acggggtcac cggcctctca
      961 cgtctcgcgg tcgaccacct ggtggcgaac tactaccaca ccgaggaggc caaggagatg
     1021 ggccgcgcct tcaacagcaa gcaggacccc gaccccgaca agttttacag gtagttccca
     1081 ccgtataccc ctctcccctc aggggagagg gcagggtgag gggccgttga aaactaccgc
     1141 tcccgcgcca gcaccgtccg cgcccgcggc aggtccgccg ccgccaccag gagcaccacc
     1201 tccgcctggt cgccgacgct gaaggggtac acggactggg cgagccggct ctggagcacc
     1261 acgcggatgc cctcgctctc gaggaggccg cgcacgacga gggcctccgc ctgatcgcgg
     1321 cagcgccgcg cttccacgag cgttcccgcg ggcaggctgc tctcgggcgg acggccggtg
     1381 ggcggaccac cggcgggcgc cccggcgcgg gcggcgggac ggaccggaaa cgggatgatc
     1441 ttggcgcggc gtgcgggcat cgggctcatg ctagcacgtc cggcggccgt ctcgaccgga
     1501 gccccgcgaa gcacgcgagc gcggccacgc cggccagcgc gatggcggtg gccatcggcc
     1561 gcgcggtgcc gtcgaagaag tggccgaccg ccatgctgta gagggcggag ccggtcagct
     1621 ggagaaagcc cgcgaccgcg gcggccagcc cggccatgcg cgggaagggg ccgatcgcgc
     1681 ccgccatccc gttcggcagg gtgagcccga ggcccaccgc gaacacgtac atcggcgcga
     1741 ccaccgcggc caccgtgagg acgccgctcc aggcgagcgc ggccaggagg aggcccgcgc
     1801 ccgcgccgag cgtggtcccg acgaggatgg tgcggtcgag gccgatccgc ccgccgaacc
     1861 gtccggagag gaacgtgccg gtcatgatcc cgagggccac cgtcccgaag cagaggccga
     1921 aggtgctcgg cgagacgccg agcacgccga tcagcacgaa ggacgagccg gagatgaacg
     1981 cgaactgccc gccgaacatc agggcgttga ccagcacgta gccgagatag ctccggtcgc
     2041 cgagcaggac cgcgaggttg cggcccatct gcccgggctg gagcgcggtc gggtcgcgcc
     2101 gcacgttggt ctccggcacg cccaccacca tcagcacgac gaagagcgcg ccgagcgcgg
     2161 tcagcagcca gaagatcgcg cgccagccga aggcctcgtg caggtagccg cccgcgatgg
     2221 gcgccagcat cgggttcagc gactgggcca tgctcatgta ggagaagacg cgcgcggcgc
     2281 gctcgcggtc gtagagatcg cgcaccaccg cgcgcgccac caccgggccc gcgccgccac
     2341 ccagggcctg gagcacccgc gccgcgatca gcacgcccac tccgggcgcc agcgtgcagg
     2401 cgagcccggc ggcggtatag aggccgaggc ccccgaccag cacccaccgg cggccgtagc
     2461 gatcggagag cgggccgtac acgagctggg ccgccgcgaa gcccatcagg aacagggtca
     2521 cggtgagctg cacggtggcg ggcggcgccg cgaaggcgac cgcgatcgcg gggagcgacg
     2581 gcaggaacat gtcgatggag agcgcaccca ggcccaccac cgcggtcagc aggaactcga
     2641 cgcgcacctc gctcatatat cccacttgga ggcttggggg tagactgtca cgggcccagg
     2701 cccggaaagg agcttcactc atgcgcgcct ttgcgatccg gatgggggcg atcctgctgg
     2761 gggccgtctc gctgctggtg ggcgcgggcg ccgctcctgc cgcggcccag gggccgatca
     2821 agatcgggtt cctctcgccg ctctccggcg ccatcgccca agccggcaag gacatgtaca
     2881 gcggctgcga gctgtactgg caggagcacg gcatgcagat cgccgggcgc aagctccagg
     2941 tgatcctcga ggacaccgag ggccagcccg ccaccgcggt ggcgaagctg cggaagctca
     3001 tcgacagcga ccgcgtcaac ctgctcgcgg gagtcatcct ctccaacgtg gcctacgccc
     3061 tggtgccgcc gatcgaggcg gccggcatcc ccaccatcta cccgatcaac tcggcggacg
     3121 acctgaccca gcgcaagcgg cccaagtggc tcgtgcgcac cggcttctcg gcgggcggca
     3181 acatgcaccc gttcggggag tacgcagcga agaccctcaa gtacaagaag gtggtcacga
     3241 tcgggctcga ctacgcgttc ggatgggaga cggtcggcgg cttccacaag tccttcgagg
     3301 acaacggcgg gcaggtgatc cagaagatct gggtgccgct caacgtccag gactacgcgc
     3361 cctacctggc ccaggtgaag cgcgacgcgg acgcggtctt cgtggtggcc ctcgggcgct
     3421 ggagcgtgct cttctccaag cagtacgccg atagcggcct caaggggcgg atcccgctga
     3481 tcgcgggcgg gacctacaac gacgagcacg tgctctcgca gctgggcgac gaggcgatcg
     3541 gggtgatcag cgcgcaccac tactctgcgg cgctcgacac cccggccaac cgcaccttcc
     3601 gcgccgcctt cgagaaggcc tactcgcgcc cgccgtcctt ctactcggag aactgctaca
     3661 ccggcgcgcg ggtcatcgag gaagcggtga aggcggtcgg cggtcgcgtg gaggaccggg
     3721 ccgccctcat ggccgccctg cgccgggtgg agatcaagga cgcgccgcgc gggccggtca
     3781 agatcgacga gtacggcaac ccgacccaga acatctacat ccgcaaggtc gagaaggtcg
     3841 gcggcgtgct ccagaacacg gtcatctaca cctatccggc ggtcagccag ttctggacct
     3901 acgaccccca gaagttcctc gagcagccgg tgtactcgcg ggagttcccg ccctgccggc
     3961 actgctagac gcccgcgcgc gaagccggcg gagctgcggg ccgcgctgag ccccttctcg
     4021 acgcccccgt cccacgatag aatgcggccg tgaaccccgg cctctccacc cgcgacgccc
     4081 agctccccgc cgcctactcg gcgcgcctcg acgcgctcgc ggtggccccc ctctggaccg
     4141 tgctccactc cctcctgccg cacgagcgga cgacgcgcgt gctcccgcac cactggcggt
     4201 ggcgggagct gcgcgagccg ctgctcgagg cggcgcgcct ggtgccgatc gagcaggccg
     4261 agcgccgggt gctgatgctg tgcaaccccg gcctgcccgg caacgcctcg gccaccgcca
     4321 cgctctacgc cggcttccag atcatcctgc ccggcgaggc ggccccgagc caccaccaca
     4381 ccgcggccgc gctccggctg atcgtggagg gcgagggcgc ctacaccacg gtggacgggg
     4441 tgaagtgcgt gatggagcgg ggcgacctca tcatcacccc gccgatgcgc tggcacgatc
     4501 acggtcacga ggggcgcgat ccggtcatct ggcttgacgg cctcgacatc ccgatcgtgc
     4561 gcagcgtgga ggcgaactgg gcttccgcga tgaagcccgc ctcgacgccg tcgagcgacg
     4621 tcgactcctc ccaggacgag ttcaccgccg cgggcctggt gccgcgccgg tcgcgctacg
     4681 aggagaccgg ctacccgcag gtgcgctggc cgtggcggca ggtgcgcgcg gccctcgccg
     4741 cgctcgcggc taccgcgccc cggcgcacgc cggtggtgct gcgtcacgtg aacccgcgca
     4801 ccggcgagta cccgctccgc acgatgggct cggagacgcg ctggctgcgt ccgggcgagg
     4861 acgtgcgccc cgagcgccgc accgggagcg aggtcgtcta cgtggtcgag gggcggggcg
     4921 agagccggat cggcgatcgg gtgatcgcgt gggaggaggg cgacgtgctc gcggtgccgc
     4981 cgtggcactg ggtcgagcac cagagccgct ccgccggcgg ccccgcctgc ctgttccacc
     5041 tgaacgacga gcccgcgctg cgcgcgctcg ggctctggca cgaggaagcc ggcgcctagc
     5101 ccgtcgtccc gtcggagagt cgaatggaag ctcgctacga ggcgctgatg gacgtgatcc
     5161 ggggccgcat gaccaaccgc gccttcgccc ccgccgagat cccgcgcgcg cacgtggaga
     5221 tgatcctgga ggcggcgcgg cacgcgccct cgggggccaa cgcccagccc tggcactacg
     5281 tcgtcgtcac caatcctgac gtcaagcagg cgatcggcca gtccttcgtc gacgagcagc
     5341 agcgccgcgc caggctccgc atgggctttc ccaccccgaa ctacaacggc gtcaagaccg
     5401 cgccgggcct cgtggtggtg gtcgccgatt tccggttcgt gcgagccttc ccggtgctca
     5461 acgacggctc cgagaccgac cggcgctacc ggcagaatgc ggagcgcatc ctgctccagt
     5521 cggtggcggc ctccaccatg tccgcccacc tggccgcggc cgcgctcggc tacgcggtgt
     5581 ggtggatcac cgcgattggt caggacgacc tccagcaaac catccggccc ctggtgggcg
     5641 tgccggagtt cctgtccatc atcgacgtga tgtgcttcgg gccgccgctc aagccgtcct
     5701 acaagcggtg gaagaagcgc ctcgatcaga tcgtgagctg ggaccgcatg gatccggacg
     5761 acgtgatgac cgacgagcag atcgacgagt gggtgaagac cacccgccac aaggtcatgt
     5821 accgggacga ggcgagggtc gactgatgct gtccgtcacc gaggtggatc gcgtctcggt
     5881 caccaccgtg gtggacaact acgtcgacgt cctccgccag gacgagaagg tcgcccgccg
     5941 cttcagcgcg ttctcggccc gccagatgcc ggacctccga gcggagcacg gcctcgccca
     6001 ccacgtggag gttgcacgcg ggcgcgagac ggtgcgcatc gccttcgact tcgggtcgtc
     6061 cgagaccgcc ctgacgcaca acttccgggc gctgggtctc gacgcctcgg ccatcgacgt
     6121 gctcgccctg agccacgggc actgggacca ctggggcggc ctcgccggca tgctccgtac
     6181 ctaccgccgg aggatgaagc gagacctcac cttctatgga ggggaggacc acttcctggc
     6241 ccgcttcaac cagcggggtg ccgatcgcgt caacatgggc cggctccacc gcgacgagat
     6301 cgagcgctac gatatccgga tcgaggcggt gcgggcgccc ctgatgatcg ccgacggagt
     6361 gctgctgagt ggcgagatgc gcgagcagga gcccttcgag cccatccccg acaacctgaa
     6421 ggtggagcgg gatggcgcga tcgtgcccga ctcgttcatg ggcgagcaga ccctgatcgc
     6481 caacctgaag ggccgcggcc tcgtgatcgt gacctcgtgc tcgcatcgcg gcatcgtggg
     6541 catctgccgt catgccgccc gcatcaccgg ggtgccgaag gtgcacgcgg tcatcggggg
     6601 cttccacctg agcgggctca aggaggagcg cgtcacgcga gtggtggacg cgttccgcga
     6661 cctcgaggtg gactggatcg tgccgcagca ctgcaccggg ctcgaggcga tggccagcat
     6721 gatgcaccgg ctgccgcagg agatggtgcc gagctcggtc ggctcggtct tcaccttcgg
     6781 gaagtagcgg ctagagatcc cgcgcggcgg gcagccccgc ggccccggtg gccgcgcgca
     6841 cgccccgcac gatcgcgcgg gccaccaggt ccgcgagcgc gcgtcccagc gcctcgaccg
     6901 gggcgtcccc ggtgcccacc gagaggcaga agagggtgtc gccgtcggtg gcgagatgcg
     6961 gcggcgacag cgcgcgggtg aacccgtcca tggccaggcg ggcgacccgc cccgcttcga
     7021 ccttgtcgag cgcggcggtg gtggccacca ggccgatggt ggtgttgacc ggccggaacg
     7081 cgggagcggg cacgcccgcc gcgagcgccg ccgcggtgtc gatgagccgc cggccgtcgg
     7141 ccgcgtcgcg ggcgcccgcg atgagccgcc cggtctcggg atcgcgcacg tcgccgaccg
     7201 cgttcaccgc catgatcgcg cccaggtgca cgccgtcgag ctcgcccacc gcgcagccga
     7261 ggccgccctt ggtcgcgcgc gccacgccgc cgatcttgcc cacggtggcg ccggtgccgg
     7321 cgcccacgtt gccttcctcc accggcccgg gccgcgcggc cgcggcggcg cggtagccca
     7381 tggcccgatc gggccgcacg cgcgcgtcgc ccacgcccag gtcgtagaga atcgcgcccg
     7441 ccacgtgcgg caccacggtc tggctcgtgc ggaagccgac cccgcgctcc tcgaggtact
     7501 gcatgacgcc ccagatcgcc tcgaggccga aggcgctgcc cccggccagg accacgccgt
     7561 cgagcgtggg caccaggtgg cgcgggtcga ggaactcgag cccgtgcacc ccggcggccc
     7621 agccgcggat ctccacgccg ccgatcgcgg gcgcgtcgct caggatgacg gtgcagccgg
     7681 tgagcacggc cgcatcggtg acgtggccga cccgcagccc ggggatcatg agcgcgtgcg
     7741 ccgcgacggg gc
//
LOCUS       DATMCV010000993         6170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_925949, whole genome shotgun sequence.
ACCESSION   DATMCV010000993 DATMCV010000000
VERSION     DATMCV010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925949"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            490..2130
                     /locus_tag="VNU03_22725"
     CDS             490..2130
                     /locus_tag="VNU03_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP77357.1"
                     /translation="MNDLREVAFPALDDAQMAALGRCAGASLKRYQDGEKLIEVGARD
                     FSFFVVKSGEIEVRDESVEPPKLLATLGRGQFTGDVSHLTGGPSLVSAVARGECLVYE
                     VPPGGVHEILSRFPELGDLILQSFIARRQLLRKPEHFTGPRVIGSRYSRDTLRVLDFL
                     SRNRVPFTWLDLETDPQVDQLLKRFGVSEAETPVVAWSKLVLRNPSDRELAEALGIRR
                     PLEHTLYDLVVVGAGPAGLGAAVYAASEGLNTAVLERTAPGGQASRSMRIENYLGFPT
                     GVSGAELAERAVVQAGKFGARLAVATPVTALSFESSYPVLRLDGGETVTAKCLLIATG
                     ADYRRLPAEGCERFEGCGVYYAATLTEAPLCREADVVVVGGGNSAGQAVVFLAGLARR
                     VYLVVRGDDLYANMSAYLARRIEQASNVEVLLDHEVRRMAGDGHLHSVEVVNRRTGEV
                     RSLRTAALFSFIGAVPRTDWLPPEIEKDPDDFIRTGTSMAQSPRWPARRPPFLLETSR
                     PGVFAAGDVRAGSIKRVASAVGEGAMVVAFVHEYLKEI"
     gene            2131..3090
                     /locus_tag="VNU03_22730"
     CDS             2131..3090
                     /locus_tag="VNU03_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWP77358.1"
                     /translation="MIEWLNANVRSELVGVAVATGQAVLRITLVLVAGCVGVRFLRAG
                     IRRLEAVFIAAGERAEVVPGATRKRITTLTGMLRTLVLVLIWAMVTVICLSQLGFDVT
                     PIVAGAGIAGLAVGFGAQNLVRDVISGFFLVLENQVRVGDVAVVNGTGGLVETITFRT
                     IVLRDLSGVVHVFPHGTVTTLANMTMEWSAYVLDVGVPYQEDTDRVVEIMRRVADEMR
                     ADSTYRSTILEPIEIFGVDDFRESAVTIKARLKTLPIEQWAVGREYRRRLKKAFDAAG
                     VQIPFPHRSLDLVEVRRPLEILVRQAADPREAGKADDGRGRSA"
     gene            complement(3377..3484)
                     /locus_tag="VNU03_22735"
                     /pseudo
     CDS             complement(3377..3484)
                     /locus_tag="VNU03_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            3487..3756
                     /locus_tag="VNU03_22740"
                     /pseudo
     CDS             3487..3756
                     /locus_tag="VNU03_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            4072..4458
                     /locus_tag="VNU03_22745"
     CDS             4072..4458
                     /locus_tag="VNU03_22745"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP77359.1"
                     /translation="MEALLAWDWPGNVRELESAIVRALPVTEDGGSGGPGLPAMTGGA
                     AAGTRETPRTFKSQKRRILEVFERQYLIRIMTEHRGNVSRAARAAGKERRDLGKLLKR
                     HGLDPGQFSGRITPRPSLPIGAPISA"
     gene            complement(4480..5094)
                     /locus_tag="VNU03_22750"
     CDS             complement(4480..5094)
                     /locus_tag="VNU03_22750"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWP77360.1"
                     /translation="MEALLEQWRYAAIFLMVILGNMGLPVPEETVLTLGGYLASRGVT
                     RLHWVVVIGVISAVIGDNIGYWLGRRYGRAAIERYDRWVFVSPERLRTISAFVGRYGA
                     LAVFVARFVPGLRFLAGPLAGAMGMPPITFTLANALGALVYVPCAVGLGYAVGYSVGD
                     VIQGLMGRVERFAIAAIALLTLVFSARRILRTRATATREMAPPS"
     gene            5462..6118
                     /locus_tag="VNU03_22755"
     CDS             5462..6118
                     /locus_tag="VNU03_22755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWP77361.1"
                     /translation="MFFSLIGRHLDDLYHFVGHRLAYAEAVGDLRPGELQPEEVVDAV
                     VLQAYREFVKIPPAGKARSWLIGLARDQLANQMERQKRWRDRTASRIEEDVPETPPTE
                     QVSTLGDEILDFYEPDEDLKLEDVIADMSVAIPEEEAESRELRWCMDAALAGMPVVWR
                     RAVRLRHVDGLQVAEIARTLGTSEAEVGRTLEHASQYLRQKLVEWGCAEKVGRENTDG
                     "
BASE COUNT          897 a         2105 c         2259 g          909 t
ORIGIN      
        1 cgccggtcga cgactccctg ctggacgacg aggcccacca cctgtctcga ccggggatgg
       61 aggaccagcc ggctgacggt gccgacgctc ttgccttctc ggtccacgac gtgggtgccg
      121 aatggaacca gcatgcgtct ctcctccttg gccgggatgg ggtcactcgg tttgcgcacc
      181 gtgagcgccg gcatcccccc ggcggtgcaa gcgcggagcc aggccggacg cgggccccgc
      241 tgaggccggg cgcgacggct cggatctcca cgccgtccat gaccggcctg cccccatggc
      301 gtcgggagcc ggcggtcagc gcggagcggt tcccgggtgg cgagacaccc acgtgctcgg
      361 gtgtgtcggc acccgatagc cgggtggaag cccgcgctga gaccggcccc ggcacgtaga
      421 tcggccggtc agtcgggctt ccggatggcg cggctcttgc cttcgtttgt ctcgatgcgc
      481 gaatcgcaat tgaacgacct gcgcgaggtc gccttcccgg cgctcgacga cgcccagatg
      541 gccgcgctgg gacgctgcgc cggtgcctcc ctcaagcggt accaggacgg ggagaagctc
      601 atcgaagttg gcgcgcggga cttcagcttt ttcgtggtga agtccggcga gattgaggtc
      661 cgcgacgagt cggtggagcc gccgaagcta ctggcgacgc tgggccgcgg ccagttcacc
      721 ggcgacgtct cccatctgac cggcggtccg tcgctcgtga gcgctgtcgc ccggggcgaa
      781 tgcctcgtct acgaggtgcc gccggggggc gtgcacgaga tcctctcccg cttccccgag
      841 ctgggcgacc tcatcttaca gtccttcatc gctcgacggc agctcctgcg gaagccggag
      901 cacttcaccg gaccgcgggt gatcggctcg cgctactcgc gcgacacctt gcgcgtcctc
      961 gacttcctct cgaggaaccg ggtgccgttc acctggctcg acctggagac cgacccgcag
     1021 gtggaccagc tgctgaagcg gttcggggtg agcgaggccg agacgccggt ggtggcctgg
     1081 agcaagctcg tcctgcgcaa tccctcggat cgggagctgg ccgaggcgct cgggatccgg
     1141 cggccgctcg agcacacgct ctacgacctc gtcgtggtcg gagccggtcc ggccggactg
     1201 ggcgcggcgg tctatgcggc gtccgagggc ctcaacacgg cggtgctcga gcgcaccgcg
     1261 cccggcggcc aggccagtcg cagcatgcgc atcgagaact atctcgggtt tcccaccggc
     1321 gtgagcggcg ccgagttggc cgagcgggcg gtggtgcagg ccggcaagtt cggggcgcgc
     1381 ctggccgtcg cgacgccggt gacggccctg tccttcgaga gctcgtaccc ggtcctccgg
     1441 ctcgacggcg gcgagacggt caccgccaag tgcctgctca tcgcgaccgg agccgactac
     1501 cgccgtctgc ccgcggaagg gtgcgagcgg ttcgagggct gcggcgtcta ctacgcggcc
     1561 accctgaccg aggccccgct ctgtcgcgag gcggacgtgg tggtggtggg cggcggcaac
     1621 tccgctggtc aggccgtcgt cttcctcgcc gggctggcgc gacgggtgta cctcgtggtc
     1681 cgcggcgatg atctctacgc gaacatgtcc gcttacctgg cccgtcggat cgagcaggct
     1741 tcgaacgtgg aggtgctgct cgaccacgag gttcgccgca tggccggcga cggccacctc
     1801 cactcggtgg aggtcgtgaa ccgccggacc ggagaggtgc ggtcgctccg gaccgccgcg
     1861 ctgttcagct tcatcggggc ggtgccgcgg accgactggc tgcccccgga gatcgagaag
     1921 gaccccgacg atttcatccg aaccgggacg tcgatggcgc agtcgccgcg ctggccggcc
     1981 cggcggccgc ccttcctgct ggagacgagc cgcccgggcg tcttcgcggc gggcgacgtg
     2041 cgcgcaggct cgatcaagcg cgtggcctcg gcggtcggcg aaggggcgat ggtcgtggcc
     2101 ttcgtgcacg aatatctgaa ggagatctga gtgatcgagt ggctcaacgc gaatgtccgg
     2161 tccgagctgg tcggggtggc ggtggccacc ggccaggcgg tcctgcgtat caccctcgtg
     2221 ctcgtcgccg gctgcgtcgg cgtgcgcttc ctccgggccg ggatccgccg cctggaggcg
     2281 gtgttcatcg cggcgggcga gcgggcggag gtcgtgcccg gggccacccg caagcggatc
     2341 acgacgctga ccggcatgct caggacgctg gtcctcgtcc tgatctgggc catggtgacg
     2401 gtgatctgcc tctcccagct cgggttcgat gtgactccga tcgtggccgg ggcggggatc
     2461 gcggggctgg ccgtcggctt cggcgcccag aatctcgtcc gcgacgtgat cagcggcttc
     2521 ttcctggttc tggagaatca ggtccgggtc ggcgacgtgg cggtggtgaa cgggacggga
     2581 gggctcgtgg agacgattac cttccgcacc atcgtcctgc gcgatctctc cggggtcgtg
     2641 cacgtgttcc cccacggcac cgtcaccacg ctcgcgaaca tgacgatgga gtggtcggcg
     2701 tacgtgctgg acgtgggcgt cccctaccag gaggacaccg atcgggtcgt cgagatcatg
     2761 cgccgcgtcg ccgacgagat gcgggcggac tcgacctaca gatcgacgat cctcgagccg
     2821 atcgagatct tcggcgtcga cgacttccgg gaatccgcgg tcacgatcaa ggcgcgcctg
     2881 aagaccctgc ccatcgagca gtgggcggtc ggccgggagt accggcgccg cctcaagaag
     2941 gccttcgacg cggcgggcgt ccagattccg ttcccccacc gctccctcga cctcgtcgag
     3001 gtccgacgac cgctcgagat cctcgtgagg caggcggcgg acccccggga ggcagggaag
     3061 gcagacgatg ggagggggcg gagcgcgtga gcgccgagcg tgacgccgag cggttcgccc
     3121 ccatctgagc gggtgagctc ggcggtgcgg gcccgcggtg ggtgcgcgac cacccgctcc
     3181 ggagcggggc tccgcgcgga cgcgggtctc gtggcctcac ggctcctcgc cgggaagtcg
     3241 cggcgcccac ccgccgcagg gcgtgggtgc gatcgcgccc tgagccaaac gccgcgccga
     3301 tgctggcggt ccccgcgtgt ccatcgtcgc gggccgcgat ccggggggtg ggtgcgcatc
     3361 tggccgcgcc ggaaaacagg ggcacgtcac cgtggccggt caggaagatg atcgggatcg
     3421 gcgcgcccga cttcatgagg tcgcgctgca ggtcgaggcc gctcagggcg ggcaggcgga
     3481 cgtcgcctgc tcaggtcggt tggcctgcgt gcgcagacct tccgctccgc ccatgagttc
     3541 gtggtccatt cccggcagga tgcgccggcc tgcgtcgtgc tgtcggtgca ggccatgaag
     3601 gcgggtgcgg tggagttcct gaccaagccg ttccgggacc agcagctgct cgacgccatc
     3661 cacgaggcga tcgcgcggga ccggaccgag cggaccgagc gggccgcgtt gcggcagcgg
     3721 tacgagtccc tgagcccccg ggagcgcgag gtcattcctc gacgaagcgg acaccctcac
     3781 cccgcgcggc caggtcgcgc ttctgtgggc cctccaggac cgcacgttcc ggccgctcgg
     3841 ctcgaccacg gagcaccggg tggacgagtt cctcgccgcc acgaacgcgc ggctcgagcg
     3901 catggtcgac ggtgaggcgt tcccggccgc cctctactac cggctctgcg tattctcggt
     3961 taccgtgcct ccgctgcgcg cccggcgcga ggacatcctg ccgcccgcct atcacttctt
     4021 ggggaagtat ggggcgagct ccaccccggc gtcggacctc tcgccggggc ggtggaggcc
     4081 ctcctggcct gggactggcc cggcaacgtg cgtgagctgg agagcgcgat cgtgcgggcg
     4141 ctcccggtga ccgaggacgg gggcagcggc ggacctgggc tccccgccat gaccggcggc
     4201 gcggcggctg gtaccaggga gacgccgcgc acgttcaagt cgcagaagcg gcggatcctc
     4261 gaggtgttcg agcgccagta cctcatccgg atcatgaccg agcaccgtgg caacgtgagc
     4321 cgggccgccc gcgccgccgg caaggagcgg cgtgatctcg gcaagctgct gaagcgtcat
     4381 gggctcgatc cgggccagtt ctcggggagg atcacgccgc ggccgtcgct gccgatcgga
     4441 gctccgatta gcgcctgacg gtggcgtcgc ttgtgaggct cacgacggcg gggccatttc
     4501 ccgcgtcgcg gtcgctcggg tccggaggat ccggcgggcc gagaagacga gggtcagcag
     4561 ggcgatcgcc gcgatcgcga aacgctcgac tctgcccatc agaccctgga tcacgtcgcc
     4621 cacgctgtag cccaccgcgt agccgagacc gaccgcgcag ggaacataga ccagcgcgcc
     4681 gagcgcgttc gcgagcgtga aggtgatcgg aggcatcccc atggcgcctg ccagcgggcc
     4741 ggccaggaac cggaggcccg ggacgaagcg ggctacgaag accgccagcg ctccgtagcg
     4801 ccccacgaag gccgagatcg tgcggagccg ctcgggggac acgaagaccc agcggtcgta
     4861 gcgctcgatg gccgcgcggc cgtagcgacg gcccagccag tagccaatgt tgtcgccgat
     4921 cacggcgctg atcaccccga tgaccaccac ccagtgcagg cgagtgaccc cgcgagaagc
     4981 gagatagccc cccagcgtga gcacggtctc ctcggggacg ggcaggccca tgttgcccag
     5041 gatgaccatc aggaagatgg cggcgtagcg ccactgctcg aggagtgcct ccatgacgag
     5101 agccgcgttc ttcctcgtgg cccgcacccg tccgggcggg tgacatttca cccaccgcca
     5161 acggagcaag gctcaagcgc atcgtgatcc ccaccggggc ccgcgccccc gcctcctcgg
     5221 gtgtgtcttc acccaacgcc ggcggtcgcg cccgacgaaa cccccggatc agcggcccgg
     5281 atcagcggga agatgtcccc gcgtccgggc tggcgtcgcc cttgcacgca cgctgcacgg
     5341 cgaatgccag tcgactggag gagcgggccg atggcgaatc tagaaggctg aggggaggaa
     5401 cgcgccggtg gcaaacctcg acccgagaaa cgcgatagcc gttggacggc ggagccgggc
     5461 gatgttcttc tcgctgatcg gccggcatct ggacgatctc tatcacttcg tcgggcatcg
     5521 gctcgcctac gccgaggcgg tgggcgacct acggccgggc gagctccagc cggaagaagt
     5581 cgtcgacgcg gtcgtgctcc aggcgtaccg cgagttcgtc aagatcccgc cggcgggaaa
     5641 ggcgcggagc tggctcatcg ggctcgcccg ggatcagctg gccaaccaga tggagcgcca
     5701 gaagcgctgg cgcgatcgca ccgcgagccg catcgaggag gacgtgccgg agacgccacc
     5761 gaccgagcag gtctccacgc tcggagacga gatcctcgac ttctacgagc cggacgagga
     5821 cctgaagctc gaggatgtca tcgccgacat gagcgtggcg attccggagg aggaggccga
     5881 atcgcgcgag ctccggtggt gcatggacgc cgcgctggcc gggatgccgg tggtctggcg
     5941 acgagccgtc cggctccggc acgtcgacgg gctccaggtc gccgagatcg cgcggaccct
     6001 cggcacctcc gaggcggagg tcgggcgcac gctcgagcat gcgagccagt acctgcgcca
     6061 gaagctggtg gagtggggct gcgccgagaa ggtcggaaga gagaacaccg atggctgacc
     6121 ccctgatgat ccgctgcccg gcctgtggcg cgaccaaccg ggtcccaccg
//
LOCUS       DATMCV010000994         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_52175, whole genome shotgun sequence.
ACCESSION   DATMCV010000994 DATMCV010000000
VERSION     DATMCV010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52175"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VNU03_22760"
     CDS             complement(<1..326)
                     /locus_tag="VNU03_22760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77362.1"
                     /translation="MRPFEEMVAAELLGLLRAGAPARAVRLTVRELVVARLERGPLGP
                     LEVTDATESVMRAACHLVRSVEAPEELVEIVCRATLEVIRGHGGESTRWLPAATSAAR
                     VVLDELA"
     gene            complement(323..>2405)
                     /gene="clpB"
                     /locus_tag="VNU03_22765"
     CDS             complement(323..>2405)
                     /gene="clpB"
                     /locus_tag="VNU03_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HWP77363.1"
                     /translation="ARSGRLDPVIGRDSEIRRVIQILSRKTKNNPVLVGDPGVGKTAI
                     VEGLAQRIVRGDVPEGLKGKRIVALDMGALVAGAKYRGEFEERLKAVLKAVQDSQGEI
                     VLFIDELHTVVGAGAAEGAMDASNLLKPALARGELHCIGATTLDEYKKHIEKDAALER
                     RFQPVLVDQPSVEDTVSILRGLRERYESHHGVRIKDSALVAAAVLSDRYITERFLPDK
                     AIDLVDEAAARLRTEIDSMPAELDEITRRTLQLEIEREALKKEPDPASRERLQRIEKE
                     LTGLQEQAKTLREQWEREKAAIEKLRQLRKRIEEVKLEIEQATRAYNLNRVAELQYGT
                     LPGLERQLKEEEARVVSAAGGRRLIKEEVDEEDIAEVVARWTGIPVSKLMEGEMQKLL
                     RLEDELHHRVVGQDEAVRAVADAVVRARAGIKDPKRPIGSFLFLGPTGVGKTELARAL
                     AEILFDDENSMIRLDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRKPYSVVLL
                     DEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVNFKNTVIIMTSNIGSQLILEMRGADEK
                     SYERMREQVLEALRRQFRPEFLNRVDEVVVFRALTQAELARIVDIQLEGLRRRLGERR
                     ITVDVTEAAKQHLARVGYDPVFGARPLKRAIQREVETSIARLIVAGKVTDGGSVRVDT
                     AGDALRVEPGT"
BASE COUNT          311 a          910 c          788 g          396 t
ORIGIN      
        1 gccagctcgt ccagcacgac gcgcgccgcg ctggtcgctg cgggcagcca gcgcgtggat
       61 tcgcccccgt gtccccggat cacctcgagg gtggcgcgac acacaatctc caccagctcc
      121 tccggcgcct ccaccgaccg gacgaggtgg caggccgccc gcatcaccga ctccgtggcg
      181 tcggtcacct cgagcgggcc gagcgggccg cgctcgagcc gggcgaccac cagctcgcgc
      241 accgtcagcc ggaccgcccg cgccggcgcc cccgcgcgca gcagccccag cagttccgcc
      301 gccaccatct cctcgaatgg cctcacgtac ccggctccac gcgcagcgcg tcccccgcgg
      361 tgtccacccg gacgctgccg ccgtcggtga ccttgccggc cacgatcagc cgggcgatcg
      421 aggtctcgac ctcgcgctgg atcgcgcgct tgagcgggcg cgccccgaac accgggtcgt
      481 agcccacgcg ggccaggtgc tgcttggccg cctcggtgac gtcgacggtg atccggcgct
      541 cgccgaggcg ccgacgcagc ccctcgagct ggatgtccac gatgcgggcc agctcggcct
      601 gggtcagggc tcggaacacc accacctcgt ccacccgatt caggaactcg gggcggaact
      661 ggcggcgcag cgcctccagc acttgctcgc gcatccgctc gtagctcttc tcgtcggccc
      721 cgcgcatctc gaggatgagc tggctgccga tgttcgaggt catgatgatc acggtgttct
      781 tgaagttcac ggtgcggccc tgcccgtcgg tgagccgtcc gtcgtccagc acctgcaaca
      841 gcacgttgaa cacgtcgggg tgggccttct cgatctcgtc gagcagcacc accgagtagg
      901 gcttgcgccg caccgcctcg gtgagctggc cgccctcctc gtaccccacg tagccggggg
      961 gcgccccgat gagccgggcc accgtgtgct tctccatgta ctcggacatg tcgagacgga
     1021 tcatgctgtt ctcgtcgtcg aacaggatct cggcgagggc gcgcgccagc tccgtcttgc
     1081 ccaccccggt cggacccagg aagaggaacg acccgatcgg ccgcttgggg tccttgatgc
     1141 ccgcgcgagc ccgcaccacc gcgtcggcga ccgcgcgcac cgcctcgtcc tggcccacca
     1201 cccggtggtg cagctcgtcc tcgaggcgga ggagcttctg catctccccc tccatcagct
     1261 tggacaccgg gataccggtc cagcgggcga cgacctccgc gatgtcctcc tcgtccacct
     1321 cctccttgat gaggcgccgc ccccccgcgg cggagacgac ccgcgcctcc tcttccttga
     1381 gctgtcgctc cagtccgggg agggtgccgt actgcagctc ggccacccgg ttcaggttgt
     1441 aggcccgggt cgcctgctcg atctcgagct tgacctcctc gatgcgcttg cggagctggc
     1501 gaagcttctc gatcgccgcc ttctcccgct cccactgctc gcggagcgtc ttggcctgct
     1561 cctggagtcc ggtcagctcc ttctcgatcc gctgcaggcg ctcgcgggac gcgggatccg
     1621 gctccttctt caaagcctcg cgctcgatct cgagctgcag cgtgcggcgg gtgatctcgt
     1681 cgagctcggc gggcatcgag tcgatctcgg tgcgcagccg ggccgccgcc tcgtccacca
     1741 gatcgatagc cttgtcgggc agaaagcgct cggtgatgta ccggtcggag aggaccgcgg
     1801 ccgcgacgag ggccgagtcc ttgatgcgga ccccgtggtg gctctcgtag cgctcgcgca
     1861 ggccccggag gatcgagacc gtgtcctcca cgctcggctg gtcgaccagg accggctgga
     1921 agcgccgctc gagcgcggcg tccttctcga tgtgcttctt gtactcgtcg agggtggtgg
     1981 ccccgatgca gtgcagctcg ccgcgggcca gcgcgggctt gagcaggttg ctcgcgtcca
     2041 tcgccccctc cgccgcccct gcgccgacca cggtgtgcag ctcgtcgatg aagaggacga
     2101 tctcgccctg cgagtcctgg acggccttga ggacggcctt cagccgctcc tcgaactcgc
     2161 cccggtactt ggcgccggcc accagcgcgc ccatgtcgag cgcgacgatg cgcttgccct
     2221 tgaggccctc ggggacgtcg ccgcgcacga tccgctgggc caggccctcg acgatcgcgg
     2281 tcttgccgac gccggggtcg ccgaccagca cgggattgtt cttcgtcttg cgagacagga
     2341 tctggatcac acgtcggatc tcggagtcgc ggccgatcac gggatcgagc cggcccgagc
     2401 gggcg
//
LOCUS       DATMCV010000995         6042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_121955, whole genome shotgun sequence.
ACCESSION   DATMCV010000995 DATMCV010000000
VERSION     DATMCV010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121955"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VNU03_22770"
     CDS             <1..219
                     /locus_tag="VNU03_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter ATP-binding protein"
                     /protein_id="HWP77364.1"
                     /translation="QEAVFMADRVIVLSRRPGEVLDVVPIELPRPRREGQQGDPIFVQ
                     TVNRIWGLIKSQAQAALLDDEVVAERGG"
     gene            219..1016
                     /locus_tag="VNU03_22775"
     CDS             219..1016
                     /locus_tag="VNU03_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77365.1"
                     /translation="MAVERLVVVDRTAFLRRPDYRVVRVLALLGIVLVWELVTRTGWV
                     SSLFLPAPSGVLAEGLDMARSGQLLVHLITSLERLAWGFGVGAVAGVSVGVAVGFFAL
                     ADAVVQPIIAATFPIPKIALLPLLILWLGIGEASKVAVVALGVFFPMAINTYAGVRQA
                     DPLLIRAAVSFGAGRWSVIRKVILPSALPMVFAGLKLGAGTALLLLVAAEQIAADAGI
                     GFLILQSGNLMETTKLMVGIVVLSLLGVASYWAIGALERAVIRWRHT"
     gene            1059..2117
                     /locus_tag="VNU03_22780"
     CDS             1059..2117
                     /locus_tag="VNU03_22780"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77366.1"
                     /translation="MLLTRLRGLVALAVALVIGLTVVAIAGAQSTPAPRPAPSAMKVG
                     VLKLTSSAPIFVGVEKGFFKEFGVEPELVYFQAAAPIATALAAGQLDVGATGLTAALY
                     NIVLGGEKLWIVADKGREWPGYPLVAIVVQKEAWDGGLRSIKDLRGKRIGVTQLGSTF
                     HYHIGNILEKDGLRLSDVKIVPLQAMAATVEALKGKQVDAILVPQPFPGAAEAQGFGK
                     ILAWAGDLYPWQIATVFYSDKLATDRARAVAFMKGYVKASRYYFDAALVQKDGRAVHG
                     ANYDEVVEITARYTGVRPEIIKLGFPFQDRNGRLLVPDIERQMSWWVANGFMKKALPL
                     KALVDTSFVEEAVKGLDR"
     gene            2227..3732
                     /gene="accC"
                     /locus_tag="VNU03_22785"
     CDS             2227..3732
                     /gene="accC"
                     /locus_tag="VNU03_22785"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWP77367.1"
                     /translation="MSPPFKKVLIANRGEIAVRVIRACRELGIATVAVFSEVDRESLH
                     VLAADEAVPIGPAPATESYLVIERLIAAARSTGAEAVHPGYGFLAENAAFAEACQAAG
                     LTFIGPPPAAIRAMGDKMSARRVAIKLGVPVVPGTEQPVTDDGEAARVAAQVGYPVML
                     KAAMGGGGKGMRLVRTAGELPSALRAARAEAGAAFGDAAVYIERYVEEPRHIEIQVLA
                     DAHGGMVHLGERECSIQRRHQKLVEESPSPFVTPEMRRRMGEAACRVAAAVGYVNAGT
                     VEFLVDRDRNFYFLEMNTRLQVEHPVTELVTGRDLVKDQLRIAAGDKLGFAQDDVVAN
                     GWAIECRINAEDPYASFIPSPGLVSGLRAPGGPWVRDDTGVYAGCTIPRFYDTLMAKL
                     IVWGPDRDAAIARMRRALAEYQVVGVQTTIPILQRIVAHPDFAAGRLSTAFMERLLAS
                     DRPEGAGHHRKVALIAAALTAYDRAGRQAPIATSPGASVWRQGLRPGWGSR"
     gene            3729..4226
                     /locus_tag="VNU03_22790"
     CDS             3729..4226
                     /locus_tag="VNU03_22790"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWP77368.1"
                     /translation="MKFEAQHGGHVVPVEIAGAAGRYTIALEGETLTVDARQTVEGIW
                     SILLDGASYVADVTEQDAGYAVDVEGERYTIRVEEESRYLIRTRGATGAERGQILRAP
                     MPGKVTLVEVTVGQTVAPGDGLIVLEAMKMENEFKAAVAGTVKEIRAQAGQTVNPGDV
                     LVVIE"
     gene            4230..4967
                     /locus_tag="VNU03_22795"
     CDS             4230..4967
                     /locus_tag="VNU03_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014320805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWP77369.1"
                     /translation="MAIAIEFKGVNKWFGEHHVLVDVDLKVDAGEVIVVCGPSGSGKS
                     TLIRCVNRLEPVQKGEISVHGEPITGNGTNLVKLRTGVGMVFQSFNLFPHMTVLQNIM
                     LAPRKVKGLGMAEAEKIARTLLERVRIPDRADYYPANLSGGQQQRVAIARALAMQPRI
                     MLFDEPTSALDPEMINEVLDVMTDLAKEGMTMMVVTHEMGFARRVASRVVFMDYGKIV
                     EVQKPEAFFAAPQSDRAKDFLSKILTH"
     gene            5004..5867
                     /locus_tag="VNU03_22800"
     CDS             5004..5867
                     /locus_tag="VNU03_22800"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWP77370.1"
                     /translation="MRRLMGLVMALAVLAAGMAPAQAQQKSTLDKIGETGTVTVGTRT
                     GSPPFGYVNAKNEWVGFSIDLVEELIRPALEKKLGKPIKVEKKESTPPTRIPLLSSGA
                     VDLIAETMTDTRTRRDSVDFSLTFFVTGAQFLVKKGSPIKGIKNIDGKRIAAQQGSTN
                     ARIIREKAPKAILREFPDQPAAFQALAQGQVDAYTNDGIQLAGLKVKSPNPAQWDIVG
                     DFYSYEPYGMAMRKGESDFRNVVNSGLMDGIESGKFFEIYDKWFGVKGELPYPMDARV
                     RSFMLLQVVPK"
BASE COUNT          912 a         2022 c         2194 g          914 t
ORIGIN      
        1 caggaggcgg tgttcatggc cgaccgggtg atcgtgctct cgcggcggcc gggcgaggtg
       61 ctcgacgtcg tgccgatcga gctgccgcgc ccgcgacgcg aggggcagca gggcgacccg
      121 atcttcgtgc agaccgtgaa ccggatctgg gggctgatca agtcgcaggc ccaggccgcg
      181 ctgctcgacg acgaggtggt ggcggagcgc gggggctgat ggcggtcgag cgcctggtcg
      241 tcgtcgatcg caccgcgttc ctgcgccgcc ccgactaccg ggtcgtgcgg gtgctggcgc
      301 tgctggggat cgtcctcgtg tgggagctgg tgacccgcac cggctgggtg tcctcgctct
      361 tcctgccggc gccgtcgggg gtgctggcgg agggcctcga catggcccgc tcgggccagc
      421 tcctcgtcca cctgatcacg agcctcgagc gcctggcctg gggcttcggc gtcggcgcgg
      481 tggcgggagt gagcgtgggc gtggcggtcg ggttcttcgc gctggccgac gcggtggtgc
      541 agccgatcat cgcggccacc tttccgatcc ccaagatcgc cctgctgccg ctgctgatcc
      601 tctggctcgg catcggcgag gcctccaagg tggcggtggt ggcgctcggc gtgttcttcc
      661 cgatggccat caacacctac gcgggcgtgc gccaggccga ccccttgctg atccgcgccg
      721 ccgtctcgtt cggggcgggg cggtggagcg tgatccggaa ggtgatcctg ccgtcggccc
      781 tgcccatggt cttcgccggg ctcaagctgg gcgcgggcac cgcgctgctc ctgctggtgg
      841 cggccgagca gattgcggcc gacgccggca tcggcttcct gatcttgcag tcgggcaacc
      901 tgatggagac caccaagctc atggtgggca tcgtcgtgct gtcgctcctg ggcgtggcct
      961 cgtactgggc catcggcgcg ctcgagcgcg cggtgatccg ctggcgccac acctgatggc
     1021 ccgccggtat cgcccacgcg tataggagga tcttgcgcat gctcctgacc cgtctccgcg
     1081 gcctcgtcgc gcttgccgtg gcgctcgtga tcggcctcac cgtcgtggcc atcgcgggcg
     1141 cccagtccac cccggccccc aggcccgccc ccagtgcgat gaaagtcggg gtgttgaagc
     1201 tcacctcgtc ggctcccatc ttcgtcggcg ttgagaaggg cttcttcaag gagttcggcg
     1261 tcgagccgga gctggtctac ttccaggccg ccgcgccgat cgccaccgcg ctggccgcgg
     1321 gccagctcga cgtgggggcc accgggctca ccgccgcgct ctacaacatc gtgctgggcg
     1381 gcgagaagct gtggatcgtg gccgacaagg ggcgcgagtg gccgggctac ccgctcgtgg
     1441 ccatcgtggt gcagaaggag gcgtgggacg ggggtctccg gtcgatcaag gatcttcggg
     1501 gcaagcgcat cggcgtcacc cagctcggat ccacgttcca ctaccacatc ggcaacatcc
     1561 tggagaagga cgggctccgc ctgtcggacg tgaagatcgt gccgctccag gccatggcgg
     1621 ccaccgtgga ggcgctcaag ggcaagcagg tggacgccat cctggtgccg cagcccttcc
     1681 ccggcgccgc ggaggcgcag ggcttcggca agatcctcgc ctgggccggg gacctctacc
     1741 cctggcagat cgccaccgtc ttctactccg acaagctcgc caccgatcgg gcgcgggcag
     1801 tggccttcat gaagggctac gtgaaggcct cgcggtacta cttcgatgcc gcgctggtgc
     1861 agaaggacgg gcgcgcggtc cacggggcca actacgatga ggtcgtcgag atcaccgccc
     1921 ggtacaccgg ggtccgaccg gagatcatca agctcgggtt ccccttccag gaccggaacg
     1981 ggcggctgct ggtccccgac atcgagcggc agatgagctg gtgggtggcg aacgggttca
     2041 tgaagaaggc cttgccgctc aaggccctgg tggacacctc gttcgtggaa gaggcggtga
     2101 aggggctcga ccgctagacc acccctcacc ctgccctctc ccctgagggg agagggagat
     2161 tggtcaggtg gggcgcgggt ttaagcgttg ctcctcaggc gccactcctg ctatgatccg
     2221 agcgtcgtga gccctccttt caagaaggtc ctcatcgcca atcgcggcga gatcgccgtc
     2281 cgggtcatcc gagcctgccg cgagctgggt atcgccaccg tcgcggtgtt ctccgaggtc
     2341 gatcgcgagt cgctgcacgt cctcgccgcc gacgaggcgg tgccgatcgg ccccgcgccc
     2401 gcgaccgaga gctacctcgt catcgagcgc ctgatcgcgg cggcgcgctc gacgggcgcc
     2461 gaggccgtgc accccggcta cggcttcctc gccgagaacg cggccttcgc ggaggcgtgc
     2521 caggcggccg ggctcacgtt catcgggccg ccgcccgcgg ccattcgcgc gatgggcgac
     2581 aagatgtcgg cgcgccgggt ggccatcaag ctcggggtgc cggtggtgcc gggcaccgag
     2641 cagccggtga ccgacgacgg cgaggcggcg cgcgtcgcgg cgcaggtcgg ctacccggtc
     2701 atgctcaagg ccgccatggg tggcggcggc aaggggatgc gcctggtgcg cacggcgggc
     2761 gagctgccgt ccgcgctgcg ggcggcccgg gccgaggcgg gggccgcctt cggcgatgcc
     2821 gcggtctaca tcgagcgcta cgtggaggag ccgcggcaca tcgagatcca ggtgctggcc
     2881 gacgcccatg gcggcatggt gcatctgggc gagcgggagt gctccatcca gcggcgccac
     2941 cagaagctgg tcgaggagag cccgtcgccc ttcgtgacgc cggagatgcg gcggcggatg
     3001 ggcgaggccg cctgccgggt ggccgcggcg gtgggctacg tgaacgcagg tacggtggag
     3061 ttcctggtgg atcgcgaccg gaacttctac ttcctcgaga tgaacacgcg cctgcaggtg
     3121 gagcacccgg tcaccgagct cgtcaccggc cgcgatctgg taaaggacca gctccgcatc
     3181 gcggcgggcg acaagctcgg cttcgctcag gacgatgtcg tcgcaaacgg ctgggccatc
     3241 gagtgccgga tcaacgccga ggacccgtac gcgtcgttca tcccgtcgcc cgggctcgtg
     3301 tcggggctgc gcgcgccggg cgggccgtgg gtgcgcgacg acaccggcgt ctacgccggc
     3361 tgcaccatcc cgcgcttcta cgacacgctg atggcgaagc tgatcgtctg ggggccggac
     3421 cgggacgcgg ccatcgcgcg catgcgccgg gccctcgccg agtaccaggt ggtgggcgtg
     3481 cagaccacca tcccgatcct ccagcgcatc gtcgcgcacc cggacttcgc ggccggccgc
     3541 ctctccaccg ccttcatgga gcgcctgctc gcctcggacc gcccggaggg cgcgggccat
     3601 caccgcaagg tggctctgat cgcggcggcc ctcaccgcct acgaccgcgc cggccgccag
     3661 gcgccgatcg caacgtcgcc cggggcgagc gtctggcgcc aggggctccg gccgggctgg
     3721 gggagccggt gaagttcgag gcccagcacg ggggccacgt cgtccccgtc gagatcgccg
     3781 gcgcggcggg gcggtacacg atcgcgctcg agggcgagac gctcaccgtc gacgcccggc
     3841 agaccgtcga gggcatctgg tccatcctgc tggacggcgc ctcctacgtg gccgacgtga
     3901 ccgagcagga cgcgggctac gcggtggacg tggagggcga gcgctacacg atccgggtgg
     3961 aggaggagtc gcgctacctc atccggaccc gcggagccac cggggccgag cgcggccaga
     4021 tcctcagggc cccgatgccg ggcaaggtga cgctggtcga ggtcacggtg gggcaaacgg
     4081 tggcaccggg cgacggcctg atcgtgctgg aggcgatgaa gatggagaac gagttcaagg
     4141 cggcggtggc gggcacggtg aaggagatcc gcgcgcaggc cgggcagacc gtcaatccgg
     4201 gcgacgtgct ggtggtgatc gagtagccca tggcgatcgc catcgagttc aagggcgtca
     4261 acaagtggtt cggcgagcac cacgtgctgg tcgacgtcga tctgaaggtg gacgcgggcg
     4321 aggtcatcgt ggtctgcggg ccctccggct cgggcaagag caccctgatc cggtgcgtga
     4381 accgtctgga gccggtccag aagggcgaga tctcggtcca cggcgagccc atcaccggca
     4441 acgggaccaa cctggtcaag ctccgcaccg gggtggggat ggtcttccaa tccttcaacc
     4501 tcttccccca catgacggtg ctgcagaaca tcatgctggc gccccggaag gtgaaggggc
     4561 tggggatggc cgaggccgag aagatcgccc gcacgctgct cgagcgggtg cgcatcccgg
     4621 accgggccga ctactacccg gccaacctct cgggcggcca gcagcagcgg gtggccatcg
     4681 cgcgcgcgct cgcgatgcag ccgcgcatca tgctgttcga cgagcccacc tccgcgctcg
     4741 atcccgagat gatcaacgag gtgctggacg tcatgaccga tctggccaag gagggcatga
     4801 ccatgatggt ggtgacccac gagatgggct tcgcccgccg ggtggccagc cgcgtggtct
     4861 tcatggacta cggcaagatc gtggaggtgc agaagccgga ggccttcttc gccgcgccgc
     4921 agtccgaccg cgcgaaggat ttcctgtcca agatcctgac gcactaatgt gatccattga
     4981 acccctcact caggaggacg tgcatgagac gactgatggg actggtgatg gcgctggcgg
     5041 tgctcgcggc ggggatggcc ccggcgcagg cccagcagaa gagcaccctc gacaagatcg
     5101 gcgagaccgg gacggtgacg gtcggcaccc gcaccgggtc gccgcccttc ggctacgtca
     5161 acgccaagaa cgagtgggtc ggcttctcca tcgacctggt cgaggagctg atccggcccg
     5221 cgctggagaa gaagctcggc aagccgatca aggtcgagaa gaaggagagc accccgccga
     5281 cccgcatccc gctgctctcg tcgggggcgg tggacctgat cgccgagacc atgaccgaca
     5341 cgcggacccg gcgtgacagc gtcgacttct cgctcacctt cttcgtcacc ggggcccagt
     5401 tcctggtcaa gaagggcagc cccatcaagg gcatcaagaa catcgacggc aagcgcatcg
     5461 cggcccagca gggctcgacc aacgcgcgga tcatccgcga gaaggcgccc aaggccatcc
     5521 tgcgcgagtt cccggaccag cccgccgcct tccaggccct ggcccagggc caggtggacg
     5581 cctacaccaa cgacggcatc cagctcgccg ggctcaaggt caagtcgccg aacccggccc
     5641 agtgggacat cgtgggcgac ttctactcct acgagcccta cggcatggcg atgcggaagg
     5701 gggagtccga cttccgcaac gtggtcaact cgggactgat ggacggcatc gagtccggca
     5761 agttcttcga gatctacgac aagtggttcg gggtcaaggg cgagctgccg tacccgatgg
     5821 acgctcgcgt gcgcagcttc atgctcctgc aggtcgtccc gaagtaggcc ctcaccccgg
     5881 ccctctcccc aagggggaga gggagccccc atgaccctct ccccagaggg gagagggtag
     5941 ggtgaggggg cagggtgagg gggcaggtga gggggagctc cacatgagat accagttcaa
     6001 ctggtcggtg ctctggaccg gcgagtccgg ccagtggctg ct
//
LOCUS       DATMCV010000996         3206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_852557, whole genome shotgun sequence.
ACCESSION   DATMCV010000996 DATMCV010000000
VERSION     DATMCV010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852557"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VNU03_22805"
     CDS             <1..837
                     /locus_tag="VNU03_22805"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWP77371.1"
                     /translation="VYCSISGFGQTGPWRERPAFAHIINAASGMMALEQGDEAAPRAS
                     NLQAADVLAAVHAMGVIMAALWRRARTGRGAHLDVSMLEALVGADSVTFASVLNGGEE
                     HGNPRPGMIVHRIGDRYMAMQIVGAPRLWERLLALMKRPELGRDPRFTSSLTRRRNWR
                     ELRAVITGWLDTFASADEALAVLTEARIPCAPVLRPAEVIAQEHLAARAFFPVLPHPA
                     RADLRVTASPYHLDGKPTHPRGPAAYRVGQHTRAVLRDLLGYKDDRIAELIAAGVVEG
                     PA"
     gene            834..1136
                     /locus_tag="VNU03_22810"
     CDS             834..1136
                     /locus_tag="VNU03_22810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77372.1"
                     /translation="MNQREEALQRTEKEIIAEKAATLGRAGERLEAALGLVAELRQAF
                     ESATDPAARAWTLAGYRQARSRALDARHTLIIQREAIGLRNHRIVDQQFPEPPVIG"
     gene            1273..1632
                     /locus_tag="VNU03_22815"
     CDS             1273..1632
                     /locus_tag="VNU03_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77373.1"
                     /translation="MIRAIALGCTLSSTVLLTCGFAWPPDYADQSRADVATCVEYARH
                     SSPHFEAAVREVDLATGRVDIERTANDARGEVAFSKCLLAVRQWRLIERNLPRAPQPD
                     PAELSGMAGAPPISVIR"
     gene            1696..2616
                     /locus_tag="VNU03_22820"
     CDS             1696..2616
                     /locus_tag="VNU03_22820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWP77374.1"
                     /translation="MKLGICLPHYGRPIEPSRLAQLAARAEEVGLDSVWVTDHVVVPR
                     DVPLIYRDDMLDPLAVLSWLAGVTERIALGTSVIVLPYRSPLPVAKLLASVDVLSGGR
                     LIVGVAVGWLEGEFAALGVPFRERGRRTDEAIELFRAAWTQDYPEIKTANHDLSGRVK
                     VSPMPLQKPRPPILVGGASDAALRRVARLGDGWHASGMAPAAFRASALAVGNHWKDAG
                     REGTPELTLRVPLLLDGIHRPAVDPALLGTRHVVRGAIASVAKELRQYQTAGCSHVAL
                     ELSYSTYPAILDTIDILAEEIRPLLDQRER"
     gene            complement(2810..>3206)
                     /locus_tag="VNU03_22825"
     CDS             complement(2810..>3206)
                     /locus_tag="VNU03_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWP77375.1"
                     /translation="PGHVRIVREGGLPVAAGENLHTLHEFRALIAAGGVTFPEPDVTN
                     CGGVTIFMKICHLAEAFNLPVTSHGAHDVTVHLLAAAPNRSYLEAHGFGLDRYITEPL
                     RLVDGRAIAPDRPGHGITFDWKGLEPLRA"
BASE COUNT          430 a         1205 c         1128 g          443 t
ORIGIN      
        1 gtgtactgct cgatctcggg cttcgggcag accgggccct ggcgcgagcg tccggccttc
       61 gcccacatca tcaacgccgc gtcgggcatg atggcgctcg agcagggcga cgaggcggcc
      121 ccgcgtgcct cgaacctgca ggccgccgac gtgctggccg ccgtccacgc catgggcgtc
      181 atcatggccg ccctctggcg ccgggcccgg accgggcggg gcgcgcacct cgacgtctcg
      241 atgctggagg cgctcgtggg cgccgacagc gtcaccttcg cctcggtgct gaacggcggc
      301 gaggagcacg gcaacccgcg ccccggcatg atcgtgcacc ggatcggcga ccgctacatg
      361 gcgatgcaga tcgtgggggc gccccggctc tgggagcggc tgctcgccct gatgaagcgg
      421 ccagagctcg gccgcgatcc ccgcttcacc tcgtcgctga cgcgccgccg gaactggcgc
      481 gagctgcgcg cggtcatcac cggctggctc gacacctttg cctccgcgga cgaggccctg
      541 gccgtgctca ccgaggcccg gatcccgtgc gcgccggtgc tgcgccccgc ggaggtgatc
      601 gcccaggagc acctcgccgc gcgcgccttc ttcccggtgc tcccccaccc ggcgcgggcc
      661 gatctgcggg tgaccgcgag cccctaccac ctggacggca agcccacgca tccgcgcggg
      721 cccgcggcct accgggtggg acagcacacg cgggcggtgc ttcgcgatct cctgggctac
      781 aaggacgacc gcatcgcgga gctgatcgcg gcgggcgtcg tcgaggggcc cgcgtgaacc
      841 agcgcgagga agcgctccag cgcaccgaga aggagatcat cgccgagaag gcggcgacgc
      901 tcggacgcgc gggcgagcgc ctcgaggccg cgctcggcct ggtcgccgag ctgcgtcagg
      961 ccttcgagag cgcgacggac ccggccgccc gcgcgtggac gctcgccggc tatcggcagg
     1021 cccggtcgcg cgcgctcgac gcgcgccaca ctctcatcat ccagcgtgag gccatcgggc
     1081 tgcgcaatca tcggatcgtc gatcagcagt tccccgagcc ccctgtcatc ggctgaccaa
     1141 gcaagaattt ccggaacgac gataggctga cgcggtaagc cgggagcagc gccagcgcaa
     1201 gtccctgatt ctccgaatgg ctaccagctg gctcgcctct tgctcccagt agggcgacgg
     1261 aaggagccag ccatgatccg ggccatcgcc ctcggttgca cgctgagcag taccgttctc
     1321 ctgacctgcg gcttcgcgtg gccgccggac tacgccgatc aatcgcgtgc cgacgtggcc
     1381 acctgcgttg agtacgcgag gcactcctcg ccccacttcg aggccgcggt gcgcgaggtg
     1441 gacctggcga ccgggcgcgt cgacatcgag cgcaccgcga acgacgcccg cggcgaggtc
     1501 gcgttctcga agtgcctcct cgcggtgcgg cagtggcggc tcatcgagcg caacctgccc
     1561 cgcgcgccgc agcccgaccc ggccgagctg tccggcatgg ccggcgcacc gcctatctcc
     1621 gtcattcgtt gacggcggcc tggcgttgac gggcccggcg ctgaccgccc ggcccgtggt
     1681 aggctccggc ccaccatgaa gctcggcatc tgcctgcccc actacggacg accgatcgag
     1741 ccgtcgcgcc ttgcccagct ggccgcgcgc gccgaggagg tcgggctcga ctcggtctgg
     1801 gtcaccgacc acgtggtcgt cccgcgcgat gtccccctga tctatcgcga cgacatgctg
     1861 gacccgctcg cggtgctgtc ctggctggcc ggcgtgaccg agcgcatcgc gctcggcacc
     1921 agcgtgatcg tgctccccta ccgctccccg ctgccggtgg ccaagctcct ggccagcgtg
     1981 gacgtgctct cgggcggccg gctcatcgtc ggggtcgcgg tgggctggct cgagggcgag
     2041 ttcgccgcgc tgggggtccc gtttcgcgag cgcggccgac gcaccgacga agcgatcgag
     2101 ctcttccgcg cggcgtggac ccaggactac cccgagatca agacggccaa tcacgacctc
     2161 tcgggccgag tgaaggtctc cccgatgccg ctgcagaaac cgcgcccacc catcctggta
     2221 ggcggcgcct ccgacgccgc gctccggcgc gtggcccgcc tcggcgatgg ctggcacgcc
     2281 tccggcatgg ccccggccgc cttccgcgcc agcgccctcg cggtcggcaa ccactggaag
     2341 gacgccggcc gcgaaggcac ccccgagctg accctccgcg tccccctgct gctcgacggc
     2401 atccaccgcc ccgccgtcga ccccgcgctc ctgggcaccc gccacgtcgt ccgcggcgcc
     2461 atcgcctccg tcgccaagga gctccgccag taccagaccg ccggctgcag ccacgtcgcc
     2521 ctcgagctca gctacagcac ctaccccgcc atcctcgaca cgatcgacat cctcgccgaa
     2581 gagatccgcc cgctcttgga ccaacgagag cgctgaggcg cttgggcgct taggcgccgg
     2641 gtgccggccg cgggcggggg gtggtggggg gcctctgacc acgacccgct gcttcgccac
     2701 ctcgcgtgag gagttcccaa tccgggaggt cggcgctgca ggcagtccgc ggcggggaac
     2761 gggtcacccg gccccccacc accccccgcc cgcggccggc acccggcggt cacgctcgca
     2821 gaggttcgag acctttccag tcgaaggtta tgccgtggcc cggtcggtcg ggcgcgatgg
     2881 cccggccgtc cacgaggcgc aggggctcgg tgatgtagcg gtcgaggccg aagccgtgag
     2941 cctctagata ggagcggttg ggggcggcgg cgagcagatg gaccgtcacg tcgtgggcgc
     3001 cgtgggaggt gacgggcagg ttgaaggcct cggcgaggtg gcagatcttc atgaagatgg
     3061 tgacgccgcc gcagttggtg acgtcgggct cggggaacgt gacgccgccc gccgcgatga
     3121 gggcgcggaa ctcgtggagc gtgtggaggt tctcgccggc cgcgaccggc aggccgcctt
     3181 cccgcacgat gcggacgtgg ccgggc
//
LOCUS       DATMCV010000997         9969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_991589, whole genome shotgun sequence.
ACCESSION   DATMCV010000997 DATMCV010000000
VERSION     DATMCV010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991589"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..741)
                     /locus_tag="VNU03_22830"
     CDS             complement(73..741)
                     /locus_tag="VNU03_22830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWP77376.1"
                     /translation="MTDYAGQFNPGFRLEPARVALVVVDMQYASASRHEGLGRLLKDQ
                     GRSEQGTARFDRIESVVVPTIQRLLGAFRERGLRIVYLTVGSELPDFSDLPPHMRPMA
                     EAVGNTRGRREHEILDALAPEPHEARLNKTTMSAFHSSGFERLVRAWGVDQLLFTGVS
                     TNSCVEGTARDAADRGFRCLLVEDGCGAASPALHDAACHNFQRLLGRVDSSARVLAEL
                     GAAR"
     gene            complement(755..2185)
                     /locus_tag="VNU03_22835"
     CDS             complement(755..2185)
                     /locus_tag="VNU03_22835"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP77377.1"
                     /translation="MLDLVIRNGQVITPQGVGLWDVGVRGEQIAVVAAPGTLPTDAAR
                     VIDAAGQIVSPGGVEPHAHLAHGIMSHPEQPSITLGPEDDTRGMACGGTTTHIDFAYV
                     RPGTDIQPVIEQRAARWKGNSYVDYAFHLTLAGALPIRVFDQIAEAIQAGHPSFKVFT
                     TNVLPPHPKRAGNRIDFGRIHFAMEKVAKAGGIMVVHGEDEDLVQFNYERFREEGRMA
                     GSNLHLVHTKLSELLAFRRTVALAKASGAGVYFVHVSAREGVETVMEARSQGLPVYGE
                     TLHQYACFNAEYYKTPRGFCSHTYPSLKFPEDQQSLWDGLVHDGLSTLATDEYPTSLE
                     LKLRGRTIEDVTGGNVGAEARIGIGFTEGVVKRGMSLTRFADVTATNAARILGLYPKK
                     GVIAPGSDADLVLIDPTIRKTLAKDDFHVTDYSPWEGWQVSGWPVMTLLRGKVIAQRG
                     RLIGSPTDGQWVARRIDPAVLRRPAC"
     gene            complement(2234..3649)
                     /locus_tag="VNU03_22840"
     CDS             complement(2234..3649)
                     /locus_tag="VNU03_22840"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP77378.1"
                     /translation="MDDLTLSSVSELAQAIRAGKVGPVEATRAYLDRIARLDPTLHAY
                     ITVDADGALAAAAVLEREAAAGAWRGPLHGVPLAHKDLFLIPGLPTSCGTRTPDYFVG
                     APPCTAVARLQAAGALTLGKLNMTELALGPFGDNAHHGDVQNPWRIGHVSGGSSSGSG
                     AAVVAGLAAGALGTDTGGSIRLPAAACGVVGLKPTYGRVSRAGVMPLSWSYDHVGPLA
                     RTVRDAALMLATIAGVDPLDATSSRQPVPDYVAALDGSVAGLRIGVAGGFYADGLDAT
                     VTAALAEAVTVLGGLGARVEPLAVPDPGPIVSGSSNVMVRAESAAIHSRLLKERPGEL
                     QPAVRDRMAPGLTVTAYDYLQGQRLRAQLTREFIDAVFSRVDVLATPTIPEPAPAQAQ
                     VKAGATADVIARMGRFSRLTRPFNALGLPALSLPCGAAPDGRPLALQLVGRPFDEATL
                     LRLGHAYERATAWHRLRPALP"
     gene            complement(3663..4373)
                     /locus_tag="VNU03_22845"
     CDS             complement(3663..4373)
                     /locus_tag="VNU03_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77379.1"
                     /translation="MSLLALRAVDVAYGDLPALTAVDLVIEPGEILSVVGANGAGKTT
                     MLRAISGLLRPRAGEILLDGVRLDRLPTHAVVEHGVVQVPEGRKIFPSLTVLENLELG
                     SYTRAARPHRRESLERVLGLFPILGERRGQAAGTMSGGQQQMLAIGRALMARPRLLML
                     DEPSLGLAPKIVQEIFRIIGEINRAGTTILLVEQNTRQALALAGRGYVLENGRVVLTG
                     AGRDLLDDEHVQRAYLGM"
     gene            complement(4370..5128)
                     /locus_tag="VNU03_22850"
     CDS             complement(4370..5128)
                     /locus_tag="VNU03_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77380.1"
                     /translation="MSAAAGTGLEIQGLTRRFGGLVAVSRFDLTVAPGEIVGLIGPNG
                     AGKTTVFHLVAGFHAPSEGAIRFEGTSLVGLKPHAICRLGLARTFQLVQPFAGLTTLE
                     NVMVGAFNRSRDVAGARRRAAEIVDFVGLGPRRDVPARHLTLPDRKRLEVARGLATEP
                     RLLLLDEVMSGLNPTEIEAIIGLIRRIHARGVSLLIIEHVMRAILALSHRLVVLHHGE
                     KIADGAPALVARDPRVIEAYLGESGGESAEAAPR"
     gene            complement(5125..6081)
                     /locus_tag="VNU03_22855"
     CDS             complement(5125..6081)
                     /locus_tag="VNU03_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77381.1"
                     /translation="MAGRIGLLLVVALLAALPPFLPKYALEVLISVLFFGYLGACWNI
                     LGGYGGQFSFGHAAFFGIGAYTSTLLFLHLGLSPWLGMLVGGGLAAAFGLFAGYLSFR
                     YGLRGPYFSLVTLAFAEMLRVIAVNWKAVGSSLGLVVPNRGSAPALFLFAEKLPYYYV
                     ILAMALAALGITRLIERSRMGYALAAIRENEDAAEAAGVDALSTKLSAMAISSFLTAL
                     GGTFYAQYFAYIDPSLTFGPAISIGGLLPAIVGGAGTVAGPLLGSFVLTPISELTRAV
                     LRGRAGADVMLYGLILILVISFLPHGLVGWFRSRRAATGRAA"
     gene            complement(6071..6943)
                     /locus_tag="VNU03_22860"
     CDS             complement(6071..6943)
                     /locus_tag="VNU03_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77382.1"
                     /translation="MTWLFLQAVVSGLLLGGVYGLVASGLSLIFGVLRIINFAHGAIM
                     MLGMYASYWLFVLVGLDPYLSILVTGPVFFGLGMLIQRIVIEPNRHAAEHNQLLLTLG
                     VALVFENLALVLWQGDFRTVRVWYSGASFLVADVLVEVPRLVAAGGAVLIALALWAFL
                     RLTDAGKAIRALSEEPEGARLVGIDVARIRGVAFGIGSACVAVAGALVTPFFYVAPDV
                     GESFNIMAFVVVVLGGMGNFLGALVGGFIVGLAESLGAAFLPGSLKQLVVFGLFVIVL
                     LLRPEGLFGGRRGR"
     gene            complement(6971..8224)
                     /locus_tag="VNU03_22865"
     CDS             complement(6971..8224)
                     /locus_tag="VNU03_22865"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77383.1"
                     /translation="MSNDQRDPVATPIGRRRFLTTIGAASAVALAAPARGRAQAREVK
                     VGYILPVTGPLAFEAQLALNGLLLAVDEINGAGGIKSLGGAKITLLPGDTQNKVELGN
                     SEAARLIDQGVTTLIGPFSSLVAFSVRQVTEKNKTPFLLLATVADNVLEGGLHYCFRM
                     QPNARAMATLTVGNMIEMAKSAGTPIKRVAIMHEDGNFGTTMGNHVEAFAGKMGYEMV
                     QRVPYNLRSPDFTAELSKVKAARPDLLVISGYYGDSKIIAETAAKLRIGVHALVGLAN
                     AAYSNPKFITENRELTDQLFDGNYWHNPQSPRARAVFDAYQKRFSSTMSNHGVQGYQV
                     MFVLKDALERAASTDREKVREAIAKTNLADHILTQDAIRFDDAGENVNATPALLQVQG
                     GKPVVVGPARFVEAKPVFPVPKWKG"
     gene            8375..8941
                     /locus_tag="VNU03_22870"
     CDS             8375..8941
                     /locus_tag="VNU03_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HWP77384.1"
                     /translation="MTALWTSFLVVVLAEMGDKTQLVALALAARYRRPWTVMLGILLA
                     TTANHALAATAGVWVSRLLPPAALAWALALSFIGFGVWTLVPDRAPATGEPTGWGPLL
                     TTTVVFFLAEMGDKTQLATIALGARFEVAGLVTVGTTLGMLVADGLVVFGGAHLTRVV
                     SATLLRRAAAALFLVLGVIALAGALAWR"
     gene            9155..>9969
                     /locus_tag="VNU03_22875"
     CDS             9155..>9969
                     /locus_tag="VNU03_22875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HWP77385.1"
                     /translation="MITVPAHRVKQFGVEFFQASFSAKDIDRLVKFEVLGYGSADEPP
                     GKGKQRANRARVNWEALEKRIGESETAYQRPVIRRKIDELVSYYRDCKDAGTLPAIPG
                     AVIITSEKRFTFTPMASQHDLGLLQIPEEHGVLRVLDGQHRLLALHAVTQAGENLGIE
                     VPAVLFDRLDARQIVELFVTINAKHTRLNPSHIVSLAGRKLYPDPNQAMAHDVIRSLN
                     EDETSPLAGEIKMLGTGRGRVSQAPLAEEIVDFLDAVEKIGGGARMNEVRQHA"
BASE COUNT         1511 a         3500 c         3607 g         1351 t
ORIGIN      
        1 tctgaccgat caggagctcc tcgtacagct tgcccgccat cgcgattctc ctccggtcgg
       61 gggcggggcg gctcagcgcg cggcgccgag ctccgcgagc acccgggcgc tcgagtccac
      121 ccggcccagc aggcgctgga agttgtggca ggccgcgtcg tgcagcgccg ggctcgccgc
      181 gccgcagccg tcctccacca gcaggcagcg gaacccgcgg tcggccgcgt cgcgcgcggt
      241 gccctccacg cacgagttgg tcgacacccc ggtgaacagg agctggtcga cgccccacgc
      301 ccgcaccagg cgctcgaagc ccgaggagtg gaacgcgctc atcgtcgtct tgttgagccg
      361 ggcctcgtgc ggctcggggg cgagcgcgtc gaggatctcg tgctcccgcc gcccccgggt
      421 attgccaacc gcctccgcca tcgggcgcat gtggggcggc aggtcgctga agtccggcag
      481 ctcggagccg accgtgaggt acacgatgcg cagcccgcgc tcccggaagg cgccgagcag
      541 gcgctggatg gtcggcacca ccaccgactc gatgcggtcg aagcgcgcgg tgccctgctc
      601 ggagcgcccc tggtccttga gcaggcgacc caggccctcg tggcgcgacg cgctcgcgta
      661 ctgcatgtcg accaccacca gcgcgacccg cgccggctcg aggcggaagc ccgggttgaa
      721 ctggccggcg tagtcggtca tgccggcggg cgagctagca ggccggccgg cggagcacgg
      781 ccggatcgat ccggcgcgcg acccactgac cgtcggtcgg gctgccgatc aggcggcccc
      841 gctgcgcgat gaccttgccc cgcaggaggg tcatgaccgg ccagcccgag acctgccagc
      901 cttcccaggg actgtagtcg gtcacgtgga agtcgtcctt cgcgagggtc ttgcggatcg
      961 tcggatcgat cagcacgagg tcggcgtcgc tcccgggggc gatgacgccc ttcttcgggt
     1021 agaggccgag gatccgcgcc gcgttggtgg cggtcacgtc ggcgaagcgg gtgagcgaca
     1081 tgccgcgctt gaccacgccc tcggtgaagc cgatgccgat gcgcgcctcc gcccccacgt
     1141 tgccgccggt gacgtcctcg atggtgcgcc cgcgcagctt gagctcgagg ctggtcgggt
     1201 actcgtcggt ggccagggtg gagagcccgt cgtgcaccag cccgtcccag agggactgct
     1261 ggtcctccgg gaacttcagc gagggatagg tgtgcgagca gaagccgcgc ggggtcttgt
     1321 agtactcggc gttgaagcac gcgtactggt ggagggtctc gccgtagacc ggcaggccct
     1381 ggctgcgcgc ctccatcacc gtctccaccc cctcgcgcgc cgagacgtgg acgaagtaga
     1441 cgcccgcccc gctcgccttg gcgagggcga cggtgcggcg gaacgcgagc agctcggaga
     1501 gcttggtgtg caccagatgg aggttgctcc ccgccatccg cccctcctcg cggaagcgct
     1561 cgtagttgaa ctggacgaga tcctcgtcct cgccgtggac caccatgatg ccgcccgcct
     1621 tggccacctt ctccatggcg aagtggatgc gcccgaagtc gatgcggttg cccgcccgct
     1681 tcgggtgtgg cggcagcacg ttggtggtga agaccttgaa gctcgggtgc ccggcctgga
     1741 tggcctccgc gatctggtcg aacacccgga tcggcagcgc gcccgcgagc gtcaggtgga
     1801 aggcgtagtc gacgtaggag ttgcccttcc accgcgccgc ccgctgctcg atcaccggtt
     1861 gaatgtcggt gcccggccgc acgtaggcga agtcgatgtg ggtggtggtg ccgccgcacg
     1921 ccatgccgcg ggtgtcgtcc tccggcccca gggtgatgga gggctgctcg ggatgcgaca
     1981 tgatcccgtg ggccaggtgg gcgtgcggct ccacgccgcc cggcgagacg atctgccccg
     2041 ccgcgtcgat gacgcgcgcg gcgtcggtgg gcagcgtgcc gggcgcggcc accaccgcga
     2101 tctgctcgcc gcgcacgccc acgtcccaga gacccacgcc ctgcggcgtg atgacctgcc
     2161 cgttgcggat caccagatcg agcacgatcg gagcctagcc caggtcgcgc atgccgtcaa
     2221 cgccgccgga tcgtcacggc agggcggggc ggagacgatg ccacgcggtc gcgcgctcgt
     2281 aggcgtggcc caggcgcagg agggtggcct cgtcgaaggg ccggcccacg agctgcagcg
     2341 cgagcgggcg accgtcgggc gcggcgccgc agggcaggga cagcgccggg aggccgaggg
     2401 cgttgaaggg ccgggtgagc cgcgagaagc ggcccatgcg cgcgatgacg tcggcggtgg
     2461 cgcccgcctt gacctgggcc tgcgcggggg ccggctccgg gatggtcggg gtggccagca
     2521 cgtcgacgcg cgagaacacc gcgtcgatga attcgcgggt gagctgcgcg cgcagccgct
     2581 ggccctgcag gtagtcgtag gcggtcacgg tgaggcccgg ggccatgcgg tcgcggaccg
     2641 ccggctgcag ctcgccgggc cgctccttga gcaggcgcga gtggatcgcc gcgctctccg
     2701 ccctcaccat cacgttgctg gagcccgaca cgatcggtcc cggatcgggg accgcgagcg
     2761 gctccacgcg cgcccccagt ccgccgagga cggtcaccgc ctccgcgagc gcggcggtca
     2821 cggtggcgtc gaggccgtcg gcgtagaacc ctccggccac gccgatccgg agcccggcga
     2881 cgctcccgtc gagagccgcc acgtagtccg gcaccggctg ccggctgctc gtcgcatcca
     2941 gcgggtccac gcccgcgatc gtggccagca tcagcgccgc gtcgcgcacg gtgcgggcga
     3001 gcgggcccac gtggtcgtag gaccaggaga gcggcatcac cccggcgcgg ctcacgcggc
     3061 cgtaggtcgg cttgagcccc accaccccgc aggccgccgc gggcaagcgg atggagccgc
     3121 cggtgtccgt gccgagcgcg cccgccgcga gccccgccac caccgccgcg cccgacccgc
     3181 tgctcgagcc acccgagacg tgaccgatgc gccatgggtt ctgcacgtcg ccgtggtggg
     3241 cgttgtcacc gaagggcccg agggccagct cggtcatgtt gagcttgccc agggtcaggg
     3301 cgccggcggc ctgcagccgc gccaccgcgg tgcacggcgg ggcgccgacg aagtagtcgg
     3361 gggtgcgggt cccgcacgag gtgggcaggc cggggatcag gaagagatcc ttgtgggcga
     3421 gcggcactcc gtggagcggg ccgcgccagg cccccgccgc cgcctcgcgc tcgagcaccg
     3481 cggccgcggc gagcgcgccg tccgcgtcga ccgtgatgta ggcgtggagc gtggggtcga
     3541 ggcgcgcgat gcggtcgagg tacgcgcggg tcgcctcgac cggccccacc ttgcccgcac
     3601 ggatggcctg cgccagctcg gatacggaag acaacgtcag atcgtccatg aggggagctc
     3661 cttcacatgc cgaggtacgc gcgctgcacg tgctcgtcgt cgaggaggtc gcgaccggct
     3721 ccggtgagca cgacgcgccc gttctcgagc acgtagccgc gcccggccag ggccagcgcc
     3781 tggcgggtgt tctgctcgac cagcaggatc gtggtgcccg cgcggttgat ctcgccgatg
     3841 atccggaaga tctcctgcac gatcttgggc gccagcccga gcgagggttc gtccagcatc
     3901 agcaggcgcg ggcgcgccat gagggcgcgg ccgatggcca gcatctgctg ctggccgccc
     3961 gacatggtgc cggcggcttg cccgcgccgc tcgcccagga tggggaagag cccgagcacg
     4021 cgctcgaggc tctcgcgccg gtgcggacgg gccgcgcggg tgtaggagcc cagctccagg
     4081 ttctcgagca cggtgaggct cgggaagatc ttgcggccct ccggcacctg cacgacgccg
     4141 tgctcgacca ccgcgtgcgt gggcagccgg tcgagccgca cgccgtccag caggatctcc
     4201 cccgcccggg ggcgcaggag accggagatc gcgcgcagca tcgtggtctt gccagcgccg
     4261 ttggcgccca ccaccgagag gatctccccc ggctcgatga ccaggtcgac cgcggtgagc
     4321 gcgggcaggt ccccgtaggc cacgtccacc gcgcggagcg cgagaaggct catcgcggcg
     4381 cggcctccgc gctctcgccc ccgctctcgc caagataggc ctcgatcacc cgcggatcgc
     4441 gcgccaccag cgcgggcgcg ccgtcggcga tcttctcgcc gtggtgcagc acgaccaggc
     4501 gatgggagag ggcgaggatc gcccgcatca cgtgctcgat gatcagcagg ctcaccccgc
     4561 gggcatggat gcggcggatc agcccgatga tggcctcgat ctcggtggga ttgagcccgg
     4621 acatcacctc atccagtagc aggagccggg gctcggtggc aaggccgcgg gccacctcga
     4681 ggcgcttgcg gtccggcagc gtcaggtgcc tcgcgggcac gtcgcgacgc gggcccaggc
     4741 ccacgaagtc gacgatctcg gccgcgcgcc gccgggcccc cgcgacgtct cgcgatcggt
     4801 tgaacgcgcc caccatcacg ttctccagcg tggtgagccc ggcgaagggc tgcacgagct
     4861 ggaaggtgcg ggccagcccg agacggcaga tcgcgtgcgg cttgagtccg accaggctcg
     4921 tgccctcgaa gcggatcgcg ccctcgctcg gcgcgtggaa gccggcgacc aggtggaaca
     4981 cggtggtctt gcccgccccg ttgggcccga tcagccccac gatctctccg ggagcgacgg
     5041 tgaggtcgaa gcggctcacc gcgacgagac cgccgaagcg ccgggtgagc ccctggatct
     5101 cgagcccggt ccccgcggcc gcgctcacgc ggccctcccg gtcgcggccc gccggctccg
     5161 gaaccatccc acgagcccgt ggggcaggaa cgagatgacc aggatcagga tgagcccgta
     5221 gagcatgacg tcggcgcccg cgcggccgcg caggacggcg cgggtcagct cggagatcgg
     5281 ggtcagcacg aaggagccga gcagcgggcc cgccaccgtg cccgccccgc ccacgatggc
     5341 gggcagcagg ccgccgatgg agatcgcggg cccgaaggtg aggctcgggt cgatgtaggc
     5401 gaagtactgc gcgtagaacg tccccccgag cgcggtgagg aacgacgaga tcgccatcgc
     5461 ggagagcttg gtggagagcg cgtcgacccc cgccgcctcc gccgcgtcct cgttctcgcg
     5521 gatcgcggcg agcgcgtagc ccatgcgcga gcgctcgatg agccgggtga tcccgagcgc
     5581 ggcgagcgcc atcgcgagga tcacgtagta gtagggcagc ttctccgcga acaggaagag
     5641 cgcgggggcg gagccgcggt tcggcaccac gaggccgagg gaggacccca ccgccttcca
     5701 gttcaccgcg atcacccgca gcatctcggc gaaggcgagg gtcacgaggg agaagtacgg
     5761 cccgcgcagg ccgtagcgga acgagagata gcccgcgaag agcccgaagg cggcggccag
     5821 cccgccgccc accagcatgc cgagccacgg gctgagaccg agatgcagga agagcagggt
     5881 ggaggtgtag gccccgatgc cgaagaaggc ggcgtgaccg aacgagaact gcccgccgta
     5941 gccgccgagg atgttccagc acgcgcccag gtagccgaag aagagcacgc tgatcagcac
     6001 ctcgagcgcg tacttgggca ggaacggcgg gagcgcggcg agcagggcga ccacgaggag
     6061 gaggccgatc ctaccggcca cggcgtcccc cgaagagacc ttccggccgc agcaggagca
     6121 cgatcacgaa gaggccgaag accacgagct gcttgaggga gccgggcagg aacgcggccc
     6181 cgagcgattc ggcgaggccc acgatgaagc cgccgaccag cgcgcccagg aagttgccca
     6241 tgccgcccag caccaccacc acgaaggcca tgatgttgaa gctctcgccc acgtcggggg
     6301 cgacgtagaa gaacggggtg acgagcgctc ccgccaccgc cacgcacgcc gagccgatac
     6361 cgaacgcgac gccgcggatg cgcgccacgt cgatgccgac caggcgggcg ccctccggct
     6421 cctcggacag ggcccggatc gccttgcccg cgtcggtcag gcgcaggaag gcccacagcg
     6481 cgagcgcgat cagcaccgcg ccccccgccg cgacgaggcg cggcacctcg accagcacgt
     6541 cggccaccag gaacgaggct cccgagtacc acacccgcac ggtgcggaag tcgccctgcc
     6601 agaggacgag ggccaggttc tcgaagacga gcgcgacgcc cagggtgagc aggagctggt
     6661 tgtgctcggc ggcgtgccgg ttgggctcga tgacgatgcg ctggatgagc atcccgaggc
     6721 cgaagaagac cgggccggtg acgaggatcg agaggtacgg atcgagcccg accagcacga
     6781 agagccagta ggaagcgtac atccccagca tcatgatcgc cccgtgggcg aagttgatga
     6841 tgcggagcac gccgaagatc agggagaggc cgctcgcgac cagcccgtag acgccgccca
     6901 ggagcaggcc gctgacgacg gcctggagga agagccaggt cacctcggct cggtcacctc
     6961 gtgaaaggga ctagcccttc cacttcggga ccgggaacac cggcttggcc tccacgaagc
     7021 gcgcggggcc caccaccacc ggcttgccgc cctgcacctg gagcagggcc ggagtggcgt
     7081 tgacgttctc gcccgcgtcg tcgaagcgga tcgcgtcctg ggtcaggatg tggtcggcga
     7141 ggttcgtctt ggcgatcgcc tcccgcacct tctcccggtc ggtcgaggcg gcgcgctcga
     7201 gcgcgtcctt gagcacgaac atcacctggt agccctgcac cccgtggttg gacatggtgg
     7261 agctgaaccg cttctggtag gcgtcgaaca ccgcgcgggc ccgcgggctc tgggggttgt
     7321 gccagtagtt gccgtcgaag agctggtcgg tgagctcgcg gttctcggtg atgaacttgg
     7381 ggttcgagta ggccgcgttg gcgagcccca ccagggcgtg gactccgatg cgcagcttgg
     7441 ccgcggtctc cgcgatgatc ttggagtcgc cgtagtagcc gctgatcacg agaagatccg
     7501 ggcgggccgc cttgaccttc gacagctcgg cggtgaagtc gggggagcgc aggttgtagg
     7561 gcacccgctg caccatctcg tagcccatct tgcccgcgaa agcctcgacg tggttgccca
     7621 tggtggtgcc gaagttgccg tcctcgtgca tgatcgcgac gcgcttgatc ggggtgcccg
     7681 cgctcttggc catctcgatc atgttgccca ccgtgagggt ggccatcgcg cgcgcgttgg
     7741 gctgcatgcg gaagcagtag tggagcccgc cctccagcac gttgtcggcc acggtggcca
     7801 gcaggaggaa cggggtcttg ttcttctctg tgacctgacg gaccgagaac gccaccaggc
     7861 tcgagaaggg gccgatcagc gtggtgaccc cctggtcgat gaggcgcgcc gcctccgagt
     7921 tgcccagctc gaccttgttc tgggtgtcgc ccggcagcag ggtgatcttc gccccgccga
     7981 gggacttgat ccctcccgct ccgttgatct cgtcgaccgc cagcagcagc ccgttgagcg
     8041 ccagctgcgc ctcgaacgcg aggggcccgg tgaccggcag gatgtagccg accttcacct
     8101 cgcgagcctg ggcccggccg cgcgccggcg cggcgagcgc gaccgcggag gcggcgccga
     8161 tcgtcgtgag gaagcggcgg cgcccgatcg gcgtcgcgac gggatctcgc tgatcgttcg
     8221 acatcgtgtc ctccggagcg gaatcgagtg aatggggcaa cgggagtggc gtgaatcgaa
     8281 cgcggcgagc ctagcatcca cccccgagcc tgtcaacgcg cggggccgcc gcgccgacga
     8341 ccgcgcgtgt agaatcgacg cgagacgacc tggaatgacc gcgctctgga cttcatttct
     8401 cgtcgtcgtc ctcgccgaga tgggtgacaa gacccagctc gtcgccctgg ccctcgcggc
     8461 gcggtatcgc cgtccgtgga ccgtgatgct cggcatcctg ctcgccacca ccgccaacca
     8521 cgcgctggcc gcgaccgccg gggtctgggt ctcgcggcta ctgcctcccg cggccctggc
     8581 ctgggcgctc gcgctgagct tcatcgggtt cggcgtctgg acgctcgtgc cggaccgcgc
     8641 gcccgcgacg ggcgagccca ccggctgggg accgctgctc accacgaccg tcgtcttctt
     8701 cctcgccgag atgggggaca agacccagct cgccaccatc gccctcgggg cgcgcttcga
     8761 ggtcgcgggc ctggtcacgg tgggcaccac gctcggcatg ctggtcgccg acggcctggt
     8821 cgtgttcggc ggcgcccacc tcacccgggt ggtgtccgcc accctccttc gccgggccgc
     8881 cgccgcactc ttccttgtac tgggggtgat cgccctcgcc ggcgcgctcg cctggcggtg
     8941 aggcgggatt catccaccgc tgtccaccgg gtttcctgta tccccaggct acccacatgt
     9001 tggggataac cggaccacct accccaactc ctgggggctg acgacagatg ggtccacgac
     9061 cgaaaaggca catgttgaca ggggtttaag cttcgactag agtgggcgag ctaccccact
     9121 gcagcgaccc agtcccccgg aggcggaacg gccgatgatc acggttcccg cgcaccgcgt
     9181 gaagcagttc ggggtcgaat tctttcaggc gtcgttctcc gccaaggata tcgaccggct
     9241 ggtcaagttc gaggtgctcg ggtatggcag cgcggacgag cccccgggca agggcaagca
     9301 gcgcgcgaat cgcgcgcgcg tgaactggga agcgctcgag aagcggatcg gcgagagcga
     9361 gaccgcgtac cagcgcccgg tcatccgccg gaagatcgac gagctggtca gctactatcg
     9421 cgactgcaag gacgcgggca ccctgcccgc gatccccggc gcggtcatca tcacctccga
     9481 gaagcgcttc accttcaccc cgatggcgag ccagcacgac ctgggcctgc tccagatccc
     9541 ggaggagcac ggcgtcctgc gcgtgctcga cggccagcac cggctgctcg ccctgcacgc
     9601 ggtgacccag gcgggcgaga acctcggcat cgaggttccg gcggtgctct tcgaccggct
     9661 ggacgcgcgc cagatcgtgg agctcttcgt caccatcaac gccaagcaca cgcggctcaa
     9721 cccgtcccac atcgtgagcc tggccgggcg caagctctac ccggatccca atcaggcgat
     9781 ggcgcacgac gtgatccgct cgctcaacga ggacgagacc tcgccgctcg cgggcgagat
     9841 caagatgctc ggcaccgggc gggggcgcgt ctcgcaggcc ccgctcgccg aggagatcgt
     9901 ggacttcctc gacgcggtgg agaagatcgg cggcggggcc cgcatgaacg aggtgcgcca
     9961 gcacgccaa
//
LOCUS       DATMCV010000998         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_654248, whole genome shotgun sequence.
ACCESSION   DATMCV010000998 DATMCV010000000
VERSION     DATMCV010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654248"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..879)
                     /locus_tag="VNU03_22880"
     CDS             complement(16..879)
                     /locus_tag="VNU03_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWP77386.1"
                     /translation="MAFETIRFEVADGVGTVTLARPDKLNALSSRMLDELLAVLDQAD
                     ADDAVRVLIVTGAGRAFCAGADLSGGPRTFDRVARGHGEAPGEHEDGGGRVTLRIFDM
                     KKPLIAAINGPAVGFGITMTLPMDIRIASTAARIGFVFARRGVVPEACSTWFLPRLVG
                     PSQAAEWVYTGRVFPAEEALAGRLVSRVVPPERLLPVARELAREIAENTSAISVALSR
                     QLMWRLLGADHPMEAHRLDSRCMDWTGRSADAREGVSAFLEKRPARFTLRPSTDMPPF
                     YPWWRERSFEP"
     gene            complement(887..2335)
                     /locus_tag="VNU03_22885"
     CDS             complement(887..2335)
                     /locus_tag="VNU03_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWP77387.1"
                     /translation="MPEPAVPLATLLVADDDPAVRQSLERALTREGYAVVLAPDGLAA
                     LERLQAGGVDLVLSDLKMPGLTGLELLREAKAVAPDVDVILLTAFGTVEEAVRAMKDG
                     ATDFLTKPFQRAQLTKVVRQALERRDLIAQNRALQKRLDDLLRQGNMIGASPAFRRLM
                     TLVQQVADSSATVLIQGESGTGKELVARAIHDRSGRREGPFIAVNCAALPETLLESEL
                     FGYEKGAFTGAAGRKEGRFELADGGTLFLDEIADLSLVTQPKILRVLQEGEFERVGGT
                     KTLRVDVRIVTATNQDLATQVRDRRFREDLFYRLNVITIQVPSLRERHEDVPLLAQHF
                     LRVYAAKNNRQLDGFTDEALGCLEAYSWPGNVRELENVVERAVVLARGLRVDAPDLPD
                     AVRERSVMLLRDGPAAPADAAAAPDGVFQVRIGTPLAEVEARLLEETLRLNHGNKTLT
                     AKMLGIDPKTVFRKLKQGEADDPALGDAPASR"
     gene            complement(2328..>2948)
                     /locus_tag="VNU03_22890"
     CDS             complement(2328..>2948)
                     /locus_tag="VNU03_22890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77388.1"
                     /translation="AIELNAELLGDMVQGRVSEDGTEATALVGAIRDQVAALDALTEE
                     YLAFARFPRPQFEEDSVNDMVSAVADFVRPLAARQGIAVKVATDAAVPPMEFDRTLLR
                     QAVLNLLKNGIEAVPQGGTLTLTTACLDETVEIAVSDSGAGIPPEVGRRLFEQFFTTK
                     PQGTGLGLSISRQIVEEHGGKIRWSSAPGVGATFTMALPIRKLEHA"
BASE COUNT          399 a         1055 c         1077 g          417 t
ORIGIN      
        1 accgcctggg gcggctcagg gctcgaagga gcgctcgcgc caccacggat agaagggcgg
       61 catgtcggtg ctggggcgca gggtgaaacg ggcggggcgc ttctcgagga acgcgctcac
      121 gccctcgcgg gcgtcggcgg agcgtcccgt ccagtccatg caccgagagt ccagccgatg
      181 cgcctccatc ggatggtccg ccccgagcag ccgccacatg agctggcgcg acagagccac
      241 cgagatcgcc gaggtgtttt ccgcgatctc ccgcgccagc tcgcgcgcca ccgggaggag
      301 ccgctcgggc gggaccaccc ggctcaccag ccggcccgcg agcgcctcct cggccgggaa
      361 cacgcgcccg gtgtagaccc actccgcggc ctggctcggc cccacgaggc gcggcaggaa
      421 ccaggtgctg cacgcctccg ggaccacccc gcgccgcgcg aacacgaagc cgatgcgcgc
      481 ggcggtcgac gcgatgcgga tgtccatcgg cagcgtcatc gtgatgccga accccaccgc
      541 gggcccgttg atggccgcga tcaacggctt cttcatgtcg aagatccgca gggtgacgcg
      601 gccgcccccg tcctcgtgct cgccgggcgc ctcgccgtgt ccccgcgcca cccggtcgaa
      661 ggtccggggc ccgcccgaga gatcggcgcc cgcgcagaac gcgcggccgg ccccggtcac
      721 gatcagcacg cggaccgcgt cgtcggcgtc ggcctggtca aggaccgcga gcagctcgtc
      781 gagcatgcgg gagctgagcg cgttgagctt gtcggggcgc gccagcgtga cggtgccgac
      841 cccgtcggcg acctcgaagc ggatggtctc gaaggccaac gcgggatcag cgggacgcgg
      901 gcgcgtcgcc cagggcgggg tcgtcggcct cgccctgctt gagcttgcgg aagacggtct
      961 tcggatcgat gccgagcatc ttggcggtga gggtcttgtt gccgtggttg agtcggagcg
     1021 tctcctcgag cagccgcgcc tcgacctcgg cgagcggcgt gccgatccgg acctggaaca
     1081 ccccgtcggg cgccgcggcc gcatccgcgg gcgcggcggg accgtcgcgg agcagcatga
     1141 ccgagcgctc gcgcaccgcg tccggcagat cgggcgcgtc gacgcgcagg ccccgggcga
     1201 gcaccaccgc gcgctcgacc acgttctcca gctcgcgcac gttgcccggc caggagtagg
     1261 cctcgaggca gcccagggcc tcgtcggtga agccgtcgag ctggcggttg ttcttcgccg
     1321 cgtagacgcg gaggaagtgc tgggccagga gcggcacgtc ctcgtggcgc tcccggagcg
     1381 acggcacctg gatggtgatg acgttcaggc ggtagaagag gtcttcccgg aagcgcctgt
     1441 cccgcacctg ggtggcgaga tcctggttgg tggcggtgac gatgcgcacg tcgacccgga
     1501 gcgtcttggt gccgcccacc cgctcgaact ctccctcctg gagcacgcgc aggatcttgg
     1561 gctgggtgac cagcgagaga tcggcgatct cgtcgaggaa cagggtgccg ccgtcggcga
     1621 gctcgaagcg cccttccttg cggccggccg ctccggtgaa ggcgcccttc tcgtagccga
     1681 agagctcgga ctcgagcagg gtctcgggca gcgcggcgca gttcaccgcg atgaaggggc
     1741 cctcgcgccg gccggagcgg tcgtggatgg cgcgcgccac cagctccttg ccggtcccgc
     1801 tctcgccctg gatcaggacg gtggccgagc tgtccgccac ctgctgcacg agcgtcatga
     1861 gccgccggaa cgcggggctc gccccgatca tgttgccctg ccgcagcagg tcgtccaggc
     1921 gcttctgcag cgcgcggttc tgggcgatga ggtcgcggcg ctcgagcgcc tggcgcacga
     1981 ccttggtgag ctgggcccgc tggaagggct tggtgaggaa gtcggtggcg ccgtccttca
     2041 tcgcccgtac cgcctcctcg acggtgccga aggcggtgag caggatcacg tcgacgtcgg
     2101 gtgccaccgc cttggcctcg cgcagcagct cgagcccggt gagcccgggc atcttgaggt
     2161 cggagagcac gaggtccacc ccgcccgcct gcagccgctc gagcgcggcg agaccgtcgg
     2221 gggccagcac caccgcgtag ccctcgcggg tcagcgcgcg ctcgaggctc tggcgcaccg
     2281 cggggtcgtc gtcggccacc agcagggtcg cgagcggcac cgcgggctca ggcatgctcg
     2341 agcttccgga tcggcagggc catcgtgaag gtggccccca cgccgggcgc gctgctccag
     2401 cggatcttgc cgccgtgctc ctcgacgatc tggcgcgaga tggagaggcc gagcccggtc
     2461 ccctgcggct tggtggtgaa gaactgctcg aagaggcggc gaccgacctc cggggggatc
     2521 ccggccccgc tgtcggacac cgcgatctcc accgtctcgt ccaggcaggc ggtggtgagg
     2581 gtgagcgtgc cgccctgcgg taccgcctcg atcccgttct tcagtaggtt caggacggcc
     2641 tgtcgcagca gcgtgcgatc gaactccatc ggaggcaccg cagcgtcggt ggccaccttc
     2701 accgcgatgc cctggcgggc cgcgagcggg cgcacgaaat ccgccaccgc gctgaccatg
     2761 tcgttgaccg agtcctcctc gaactgtggc cgtgggaagc gcgcgaaggc gaggtactcc
     2821 tcggtgagcg cgtcgagggc cgcgacctgg tcgcggatgg ccccgaccag tgcggtggcc
     2881 tccgtcccgt cctcgctcac ccgcccctgc accatgtcgc ccagcagctc cgcgttcagc
     2941 tcgatggc
//
LOCUS       DATMCV010000999         2766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_991630, whole genome shotgun sequence.
ACCESSION   DATMCV010000999 DATMCV010000000
VERSION     DATMCV010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991630"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VNU03_22895"
     CDS             complement(<1..970)
                     /locus_tag="VNU03_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77389.1"
                     /translation="MDRRGTTAVGGGSPGWIAALAVLVLLCLSRGFEISPIRPGLDPS
                     YLYALNHATVHSLSWGQDFISTYGPYGFYFTTYDLGNLPARKLALALLLAGGAGVTAT
                     LLVWREAGIPTWLRFVSLGLLLYTFVIQILEYQVVGFLVLLLLFAVREGCIKGLVAFG
                     SAGLLAGFCMLVKFSLGFGALLTVAIACLVIGRPRLILSRAGVALAGALAGLLGGWQL
                     YQGSPRGLSTFLRSGWDVAGGYSAAMGTAEIWPISVGAFLLWIGLLVVWVVSWRASRP
                     LRSLAVLAIPLFVAWKHSIVRQDVHVKILVLFGLFSMAILLLDAAVL"
     gene            complement(943..1602)
                     /locus_tag="VNU03_22900"
     CDS             complement(943..1602)
                     /locus_tag="VNU03_22900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP77390.1"
                     /translation="MRDLSKLYSVRFAPDDKARKDRVWRVLCRHFFQKYVERTDTVLD
                     IGAGHCEFINHIEARRRIAFDLNPDVRANAAVGVEVVQGVSTDLSAFHDELFDVVFVS
                     NFFEHLPTKDDLLRTLAEVRRVLRPGGKLLIVQPNIRLAGGAYWDFVDHHLPLTERSL
                     VEAVELAGLQPSEVRARFLPFTSKSLLPQHPLLVRLYLKVLPAQWLLGRQSWIVAVRP
                     R"
     gene            1777..>2766
                     /gene="tig"
                     /locus_tag="VNU03_22905"
     CDS             1777..>2766
                     /gene="tig"
                     /locus_tag="VNU03_22905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWP77391.1"
                     /translation="MKVDIEEMGACKRRLQVEETPEVVTRAWEQAFGRVQREAKLPGF
                     RKGKVPRSMIKLHFADDVRQEVARRLIPDVYRQALAETRIEPVEEPDLQEVTLEENAA
                     LKFAAVVEVKPAITLGSYAGLAIEHAPKPFAESEVDQALTALQEQHAEYRAVERAADP
                     GDLVIVDYTLTPEGTEPRSETGYGFVIGAGGVLPEMEEAVIGLAAGGSRQTRVRFADD
                     HRNEALRGKSAEASVTVSEVKEKVLPALDDDFAKSVGGNFETLDALRDEVRKGLQARR
                     EADNRRALESAVLDAVLAGHAFEVPDALVLRQVGHQIEHAREHMRRQGLDPDKV"
BASE COUNT          508 a          911 c         1004 g          343 t
ORIGIN      
        1 cgaggaccgc cgcgtcgagg agcaggatcg ccatcgagaa gagaccgaac aggacgagga
       61 tcttcacgtg gacgtcctgg cggacgatgg agtgcttcca cgcgacgaag agtggtatcg
      121 ccaggaccgc gagagaccgg aggggccgcg acgcgcgcca ggacacgacc cacacgacga
      181 gcaggccgat ccagagcagg aacgcgccca cgctgatcgg ccagatctcc gcggtgccca
      241 tcgccgcgga gtagcctccg gcaacgtccc atccgctgcg gaggaacgtc gagagccctc
      301 gcgggctgcc ctggtagagc tgccagccgc ccaggagccc ggccagcgcg ccggccagcg
      361 ccaccccggc ccgggacagg atgagacggg ggcgaccgat cacgaggcac gcgatcgcga
      421 ccgtgagcag ggcaccgaat ccgagcgaga acttgacgag catgcagaac ccggccagga
      481 gcccggccga tccgaaagcg acgagcccct tgatgcaccc ttctcgcacc gcgaagagga
      541 gcaggaggac gaggaagccg accacctggt actcgaggat ctggatgacg aaggtataga
      601 ggagcaatcc cagcgagacg aagcgcagcc aggtggggat ccctgcctcc cgccagacga
      661 gcagggtggc ggtcacgccc gccccgcccg ccaggaggag ggcgagggcg agcttacgag
      721 ccggcaggtt gcccagatcg taggtcgtga agtagaagcc gtaggggccg taggtggaga
      781 tgaagtcctg gccccaggac agcgagtgga cggtcgcgtg gttcagggcg tagaggtacg
      841 agggatcgag cccgggccgg atcgggctga tctcgaaccc gcggctcagg cacaggagga
      901 ccaggacggc gagggccgcg atccaccccg gcgagcctcc ccctaccgcg gtcgtaccgc
      961 gacgatccac gactgacggc cgagcagcca ctgggccggg agcaccttga ggtacagacg
     1021 caccagcagg gggtgctggg ggagcaggct cttggaggtg aacgggagaa acctcgcccg
     1081 cacctcgctg ggctgcagcc ctgccagctc gaccgcctcc accagcgacc gctcggtcag
     1141 agggaggtgg tgatcgacga agtcccagta ggcgccgccc gcgagccgga tgttcggctg
     1201 cacgatcagc agtttccccc cgggtcggag cacgcggcgc acctcggcga gtgtccgcag
     1261 gagatcgtcc ttcgtgggga gatgctcgaa gaagttgctc acgaagacca cgtcgaagag
     1321 ctcgtcgtgg aaggcgctca ggtcggtgct gacgccctgc acgacctcga ccccgaccgc
     1381 cgcgttggcc cgcacgtccg ggttgagatc gaacgcgatg cgccggcggg cctcgatatg
     1441 gttgatgaac tcgcagtggc cggccccgat gtcgaggacg gtatcggtcc gctccacgta
     1501 cttctggaag aaatgccggc acagcacccg ccacacgcgg tcctttcgcg ccttgtcgtc
     1561 gggcgcgaag cggaccgagt acagcttgga gagatctcgc atggcgcggt tcttccgagc
     1621 atagtccacc ctggaacccc ggggcaaact ccggggggct gccgggtaag tccctgtatt
     1681 cttgaccgtg cttcccgata gtgtaggatg gggctccggc gacgactgga gcagcatcga
     1741 ccccccttgg gagtgggatg atctcgcgga gcgatcatga aagttgatat cgaagagatg
     1801 ggggcctgta agcggcggtt gcaggtcgag gaaacccccg aggtggtcac ccgggcctgg
     1861 gagcaggcct tcggccgcgt gcagcgcgag gccaagctgc ccggcttccg caagggcaag
     1921 gtcccgcgca gcatgatcaa gctccacttc gccgacgacg tgcgccagga agtggcgcgc
     1981 cggctcatcc ccgacgtcta ccgccaggcc ctcgccgaga cccgcatcga gccggtggag
     2041 gagcccgacc tgcaggaggt gaccctcgag gagaacgcgg ccctgaagtt cgccgcggtc
     2101 gtcgaggtca agccggccat cacgctcggg tcctacgcgg ggctcgccat cgagcacgcg
     2161 cccaagccct tcgcggagag cgaggtggac caggccctca ccgcgctcca ggagcagcac
     2221 gcggagtacc gcgcggtgga gcgcgcggcc gacccgggcg acctcgtgat cgtcgactac
     2281 accctgaccc ccgaggggac ggagccgcgc tccgagaccg gctacggctt cgtgatcggc
     2341 gccggcggcg tgctgcccga gatggaggag gcggtgatcg gactcgccgc gggcggctcg
     2401 cgccagaccc gggtgcgctt cgcggacgac caccgcaacg aggcgctgcg cggcaagtcg
     2461 gccgaggcct cggtgacggt ctccgaggtg aaggagaagg tcctgcccgc cctcgacgac
     2521 gacttcgcca agagcgtggg cggcaacttc gagacgctcg acgcgctgcg agacgaggtg
     2581 cgcaagggcc tgcaggcccg gcgcgaggcc gacaaccgac gcgcgctgga gagcgcggtg
     2641 ctggacgcgg tcctggccgg ccacgccttc gaggtgccgg acgccctggt gctccggcag
     2701 gtggggcacc agatcgagca cgcgcgggag cacatgcgcc ggcaggggct cgacccggac
     2761 aaggtg
//
LOCUS       DATMCV010001000         4180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_122278, whole genome shotgun sequence.
ACCESSION   DATMCV010001000 DATMCV010000000
VERSION     DATMCV010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122278"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /gene="secF"
                     /locus_tag="VNU03_22910"
     CDS             complement(<1..911)
                     /gene="secF"
                     /locus_tag="VNU03_22910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWP77392.1"
                     /translation="MFELFHNPNFDFIGRRRWAYIISALFILAGFGSMVAKGGLRYGI
                     DFSGGTLIQVRFEKPVPVDRIRRALETVRMGESVIQEFGDTREYLLRLPAVEAASEEV
                     TKQALAALAKEPELGTPEVRRVEYVGPQVGRDLQIQAIYAALWSIAGILAYVAIRFDM
                     LGGLASVAAIVHDVLICLSAMSLTNREMSLPILAALLTIIGYSVNDTIVAYDRVRENR
                     TKGARRKGESFATQINNAINQTLSRTVLTALTVFFTTAVLYLFGGKVLEDMAFVLTIG
                     VVTGTFSTVYIAGSLIVDWQGWRERWMA"
     gene            complement(917..2473)
                     /gene="secD"
                     /locus_tag="VNU03_22915"
     CDS             complement(917..2473)
                     /gene="secD"
                     /locus_tag="VNU03_22915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWP77393.1"
                     /translation="MRRNLGLRIGLVIVVIAVSLWYLYPPRSSINLGLDLQGGIHLVL
                     GVEVDKALEAQVERAGDTVRGELEKKGIAVSKIERRGISDLAIQIASPQSWDSALTTA
                     TEVLGAFDRKEADQAAGRIVMTLKPREATQIRELAVRQGLETIRNRVDQFGVAEPSIQ
                     QQGENRILVQLPGVQDPARAKALIGKTALLEFKLVDDKVDPETAVRSGVPDGDEILYQ
                     RRVDKETHQERKVPFVVQKKAYVTGRDVATARVSIDQNTSEPYVSVDFNAAGGKAFSD
                     LTDANVGRRLAIVLDGNIHSAPQIRERIPSGRAQITGGFTSAEATDLAIVLRAGALPA
                     PVQVLEERTVGPSLGADSVRQGIIAILASTALVFVFMMAYYRLSGLIADVALGLNLLL
                     LLATMAGFHATLTLPGIAGIALTIGMAVDTNILIFERIREELRGGKTIRGAIEAGFSR
                     AFKTVIDTHVTVLVSGLILFQFGTGPVKGFAVTLMIGIAASLFTAVFFTRLLFDLLYM
                     GSHKRESLSI"
     gene            complement(2474..2809)
                     /gene="yajC"
                     /locus_tag="VNU03_22920"
     CDS             complement(2474..2809)
                     /gene="yajC"
                     /locus_tag="VNU03_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWP77394.1"
                     /translation="MVDLAYAMGPSPAGGNGGGIMSVLPLMIGMFAIMYFLIIRPQQK
                     QKRDREALLSAVKKGDRVVMSSGLYGTVVGLSEHTVTVKVAEQVKMEFERSAIGRIVP
                     VAGDKESSA"
     gene            complement(2815..3972)
                     /gene="tgt"
                     /locus_tag="VNU03_22925"
     CDS             complement(2815..3972)
                     /gene="tgt"
                     /locus_tag="VNU03_22925"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWP77395.1"
                     /translation="MSGFRFELVKTDGAARLGRIVTAHGAVDTPAFMPVATQGSVKSL
                     APSDLLAAGAEIVLANTYHLMLRPGHELVRDLGGLHRFMGWDGPILTDSGGFQVFSLS
                     KLRRIGEEGVEFRSHVDGSLRTLTPESCVAVQHALGVDVLHPLDECLAHPATVAQTER
                     SLALTERWLTRAIAAHRTAGSPGALFGIVQGGSYEPLRRRAVEAIGGLDLPGHSIGGL
                     AVGEPKPVMYDITELVAGLLPADRPRYLMGVGKPTDLVEAVARGVDLFDCVLPTRNAR
                     NGQAFTPDGPINIGNARFARDGAPLDPECACEGCRRFSRAYLRHLFAARELLAYRLMT
                     LHNLTFYLGLMRDMRRAVADGAFERFRSRFLARYGVESVGDSADSPADSEA"
     gene            complement(3976..>4180)
                     /locus_tag="VNU03_22930"
     CDS             complement(3976..>4180)
                     /locus_tag="VNU03_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HWP77396.1"
                     /translation="GPADLFIRPGHRFRVVDALVTNFHLPRSSLLALVSAFAGRERVL
                     AAYRHAVAARYRFYSYGDAMLIH"
BASE COUNT          693 a         1429 c         1455 g          603 t
ORIGIN      
        1 gccatccacc gctcgcgcca gccctgccag tccacgatca gcgagccggc gatgtagacc
       61 gtcgagaagg taccggtcac gaccccgatg gtcaggacga acgccatgtc ctccaggact
      121 ttccccccga acaggtagag aacggcggtc gtgaagaaga cggtcaaggc ggtcagcacc
      181 gtccgggaca gggtctggtt gatggcgttg ttgatctgag tcgcgaatga ctcacccttg
      241 cggcgagcgc ccttggtccg gttctcccgc acccggtcat aggcgacgat cgtgtcgttg
      301 accgagtacc cgatgatcgt cagaagggcg gccaggatgg gcagggacat ctcccggttc
      361 gtgagcgaca tggccgagag gcagatgagc acgtcgtgga cgatggccgc caccgaggcc
      421 agcccgccca gcatgtcgaa gcggatcgcg acgtaggcga ggatgccggc gatgctccag
      481 agcgcggcat agatcgcctg gatctggagg tcgcggccca cctgcgggcc cacgtactcg
      541 acgcgccgga cctcgggggt gcccagctcg ggctccttgg cgagggcggc cagcgcctgc
      601 ttggtcacct cctcggaggc ggcctcgacg gccggcagcc ggagcaggta ctcccgcgtg
      661 tcgccgaact cctggatcac gctctccccc atccgcaccg tctcgagcgc gcgccggatg
      721 cggtccaccg gcaccggctt ctcgaagcgc acctggatca gggtgccgcc cgagaagtcg
      781 atcccgtagc gcaggccgcc cttggccacc atggagccga agccggccag gatgaagaga
      841 gcggagatga tgtaggccca gcgccgccgg ccgatgaagt cgaagttggg gttgtggaag
      901 agctcgaaca tggcggtcag atcgacagcg actcgcgctt gtgcgagccc atgtacagca
      961 ggtcgaagag caggcgcgtg aagaagaccg cggtgaagag gctggccgcg atgccgatca
     1021 tgagggtcac cgcgaagccc ttgaccgggc cggtcccgaa ctggaacagg atgaggcccg
     1081 acaccaggac cgtgacgtgg gtatcgatga cggtcttgaa cgcccgggag aagcccgcct
     1141 cgatggcgcc gcggatggtc ttccccccgc ggagctcctc gcggatgcgc tcgaagatga
     1201 ggatgttcgt atccaccgcc atgccgatcg tgagcgcgat tcccgcgatg ccgggcagcg
     1261 tgagggtcgc gtggaagccg gccatggtcg cgagcagcaa cagcaggttc agcccgagcg
     1321 ccacgtcggc gatcaggccg gagaggcggt agtaggccat catgaacacg aacaccagcg
     1381 cggtcgacgc caggatcgcg atgatgccct ggcggaccga gtcggcgccg agggacggtc
     1441 ccaccgtgcg ctcctcgagc acctggaccg gggcgggcag cgcgcccgcc cgcagcacga
     1501 tggccaggtc ggtggcctcc gcggacgtga aaccgccggt gatctgggcc cgccccgacg
     1561 ggatacgctc gcggatctgc ggggcggagt ggatgttgcc gtccagcacg atggcgagcc
     1621 ggcgaccgac gttggcgtcg gtcagatccg agaaggcctt ccctcccgcc gcgttgaagt
     1681 cgaccgagac gtagggctcg ctcgtgttct gatcgatgga gacccgcgcg gtggccacgt
     1741 cgcggccggt cacgtaggcc ttcttctgga ccacgaacgg caccttgcgc tcctgatggg
     1801 tctccttgtc cacgcgtcgc tggtagagga tctcgtcccc gtccggcact ccggagcgga
     1861 ccgcggtctc ggggtcgacc ttgtcgtcca ccagcttgaa ctcgaggagc gcggtcttgc
     1921 cgatcaacgc cttcgcccgc gccggatcct ggacgcccgg gagctggacc aggatgcggt
     1981 tctcgccctg ctgctggatg gagggctcgg cgaccccgaa ctggtccacc cggttgcgga
     2041 tcgtctcgag cccctggcgc accgccagct cgcggatctg ggtcgcctcg cgaggcttga
     2101 gggtcatgac gatccggccc gccgcctggt cggcctcctt gcggtcgaag gcgcccagga
     2161 cctcggtggc cgtcgtgagc gcgctatccc agctctgggg gctcgcgatc tggatcgcga
     2221 gatcggagat cccgcggcgc tcgatcttgg agaccgcgat gcccttcttc tcgagctcgc
     2281 cgcgcaccgt atcccccgcc cgctcgacct gcgcctccag cgccttgtcg acctccaccc
     2341 cgagcacgag atggatgccg ccctggagat cgaggcccag gttgatgctg gagcggggcg
     2401 ggtagagata ccacaacgac accgcgatca ccacgatcac gaggccgatg cgaagaccga
     2461 ggttgcgccg catctaggcc gacgactcct tgtcgcccgc gacgggcacg atccggccga
     2521 tcgcggagcg ctcgaactcc atcttgacct gctcggccac cttcacggtg acggtgtgct
     2581 cggacaggcc caccacggtc ccgtagagcc cgctcgacat gacgacccga tcgcccttct
     2641 tgaccgcgga gaggagggcc tcgcggtccc tcttctgctt ctgctgcggc cggatgatca
     2701 ggaagtacat gatcgcgaac atgccgatca tcaggggcag gacggacatg atgccgccgc
     2761 cgttgccgcc ggcaggcgac ggccccattg cgtatgcgag atccaccatg aaggtcacgc
     2821 ctccgagtca gcgggtgagt cggccgagtc gcccacggat tctaccccat atcgcgcgag
     2881 aaaccgagat cggaaccgct cgaacgcgcc atcggccacc gcccgacgca tgtcccgcat
     2941 gaggcccagg tagaacgtca ggttgtgaag cgtcatcagc cgataggcga gcagctcgcg
     3001 ggccgcgaag aggtggcgaa ggtaggctcg cgagaagcgg cggcacccct cgcaggcgca
     3061 ctccgggtcg agcggcgccc cgtcccgggc gaatcgcgcg ttgccgatgt tgatcggccc
     3121 gtctggcgtg aaggcctggc cgttgcgggc gttccgggtg ggcagcacgc agtcgaagag
     3181 atcgacgccg cgcgcgaccg cctcgaccag gtcggtcggc ttcccgaccc ccatcagata
     3241 gcgcggccgg tccgcgggca ggagcccggc caccagctcg gtgatgtcgt acatcaccgg
     3301 cttgggctct cccaccgcga gcccgccgat cgaatggccg ggcaggtcca ggccgccgat
     3361 cgcttccacc gcgcggcgcc gcagcggctc gtaggagcca ccttgcacga tcccgaacaa
     3421 ggcgccgggc gaacccgcgg tccggtgcgc ggcgatcgcc cgggtgagcc agcgctcggt
     3481 cagcgccagc gagcgctcgg tctgggccac ggtggccggg tgcgccaggc actcgtcgag
     3541 cggatgcagc acgtcaacgc ccagcgcgtg ctgcaccgcc acgcacgact ccggcgtgag
     3601 ggtccggagc gagccgtcga cgtgggagcg gaactccacg ccctcctccc cgatccgccg
     3661 cagcttcgag aggctgaaga cctggaagcc gcccgagtcg gtgaggatgg gcccgtccca
     3721 gcccatgaac cgatgcaggc cgcccaggtc acgcacgagc tcgtgtcccg gccgcagcat
     3781 caggtggtag gtattggcca gcacgatctc ggcgcccgcg gcgagcagat ccgagggagc
     3841 gaggctcttg accgagccct gcgtcgccac cggcatgaag gccggcgtgt cgaccgcccc
     3901 gtgcgcggtg acgatccggc cgaggcgcgc ggcgccgtcg gtcttgacga gctcgaagcg
     3961 gaagccgctc atgcctcagt ggatcagcat cgcgtcgccg tacgagtaga agcggtagcg
     4021 ggccgccacc gcgtggcggt aggcggccag gacgcgctcg cgccccgcga aggccgacac
     4081 cagcgcgagc aggctcgagc gcggcaggtg gaagttggtg acgagcgcgt ccaccacgcg
     4141 gaagcgatgg ccgggtcgaa tgaacagatc cgccggcccc
//
LOCUS       DATMCV010001001         3997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_926476, whole genome shotgun sequence.
ACCESSION   DATMCV010001001 DATMCV010000000
VERSION     DATMCV010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926476"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..582)
                     /locus_tag="VNU03_22935"
     CDS             complement(157..582)
                     /locus_tag="VNU03_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77397.1"
                     /translation="MRRLVLATLVAILVAALAPMGAAAQEVRGGIRTEDPSTPSTGRP
                     PAPRPRPHRPRPFFFPCCVDTAYLPAQPPEPAPPPILYVLPSAPTLIYIAPARVEPAP
                     EVQLPTGRWERHGNGKEYPYTWVWVGIAGSPPLAARFNR"
     gene            951..1685
                     /locus_tag="VNU03_22940"
     CDS             951..1685
                     /locus_tag="VNU03_22940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWP77398.1"
                     /translation="MAQWDQIDVDGSPMRLYVGMPSLGRAFPAVIVIQHGPGVDRFIQ
                     DRVETLSEQHGYLAIAPELYHRQPEDGGDMMTRIGRLRDPEVVNDVSAAVNYARRLKD
                     TQLGDVGIIGFCMGGRVAYLMAASKPVFKAAGVFYGGNIMKAWGDGPTPFDLTPYVHC
                     PIVGFFGKEDANPSPADVDRLSTQLDKYRKAHEFHRYDGAGHAFLNFSNPVTYREGPA
                     RDAWQKLIPFLQRHLQSSRQPRQRLA"
     gene            complement(1696..1881)
                     /locus_tag="VNU03_22945"
     CDS             complement(1696..1881)
                     /locus_tag="VNU03_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77399.1"
                     /translation="MNLRARAVAIANQFEGRCTCSTMDGGVDCRWCQVFRELLRTHPL
                     EPRPTRSAATPAASLTP"
     gene            complement(1974..3101)
                     /gene="menC"
                     /locus_tag="VNU03_22950"
     CDS             complement(1974..3101)
                     /gene="menC"
                     /locus_tag="VNU03_22950"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HWP77400.1"
                     /translation="MKIDRIDVYWMKLPLAFVWKTSYADQSFTDTILVRMEGGGHYAW
                     GESCPPYIPAYSAEHTLATFHTLREHMAPRIVGQDIETSQDLLDRLAFIKGNQFARAA
                     LDITWWVLDAKRRGLPLHRALGGTADRVAVGADFGIQDSLDLLMAKVQGAVDAGFPRV
                     KLKFRPGWDLFMVEAVRGTFPDLTIHIDCNAAYSPADTPLFRSLDRFKLAMIEQPLAD
                     DGMSLVNHAALQRQIGTPVCLDESVQSLAHVEAAIALESCRVVNIKMARVGGLTESRL
                     IQSRCAEHGIPCWVGGMLESAVGGAICAELATLANFTYPGDIFPSSYFYADDLGKPEI
                     VLSGPGEVATSRVPGIAQEPDPALLARWTAAHATFGGPPAR"
     gene            complement(3114..3881)
                     /locus_tag="VNU03_22955"
     CDS             complement(3114..3881)
                     /locus_tag="VNU03_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP77401.1"
                     /translation="MAWDPAQYLKFADHRLRPAVDLLNRVDLDNPGEIYDLGAGAANV
                     TRLIKERWPDARVTGVDDSEAMLAKAAATAPSITWRQADLATWRPAQPADLIYSNAAL
                     HWVPGHERLFSTLLGGLAPGGVLAVQMPRNFGARSHTAISEAALAGPWRATLEPLLRP
                     APVADPDFYYGVLAPHAAALDIWETEYLQVLEGRDPVKEWTKGTWLKPLLDALAEPER
                     SRFEASYAALVARAYPPRADGRTLFPFRRLFIVARAK"
BASE COUNT          613 a         1364 c         1461 g          559 t
ORIGIN      
        1 ggccggtcaa gaacgcgatc ttcggcgaga acgtcgcgcg gctctaccgc tacgaccggc
       61 gggccgagct gggcggctcc ggtcggctcg ccgagctcaa ggcggagtac gaccgaagcg
      121 ggatcgggcg gagcaaccgg cgctacggct acgtggtcag cggttgaatc tggcggcgag
      181 cggcgggctg cccgcgatcc ccacccacac ccacgtgtac gggtattcct tcccgttgcc
      241 gtgccgctcc cagcggccgg tcggcagctg gacctcggga gcgggctcga ctcgcgcggg
      301 ggcgatgtag atcagggtcg gagcagacgg gagcacgtag aggatcggcg gcggggcggg
      361 ctccggcggc tgtgccggaa ggtacgccgt gtccacgcag caggggaaga agaagggccg
      421 gggccgatgc ggccggggtc gaggcgccgg aggccggccg gtgctcggcg tcgaggggtc
      481 ctcggtccgg atcccgccgc ggacctcctg ggccgcggcg cccatcgggg cgagcgcggc
      541 gaccaggatc gctaccagcg tggcgaggac caagcgacgc atggccggag tgtactcccg
      601 gcgagggcac tccgccagcg cacgacgcac tgccgcttga caagcagggg tggggagcgg
      661 tttgctggag ggcgacggaa ggtgaggcgc cgcctcccgt cgcgccgtta accagacaag
      721 ggagaatcag gatggacaac acgccacccg gctcgtacgg cgcgtcatcg ggcgtggttc
      781 cggcctgatg acgcgcgaag ctagctaggg cactagaaga tcccggcaca tcgtcgggac
      841 atgccgcagg atcgtgatcc tgcgcgcgag aaaccgaggc ccggcaggcg gttcaccggt
      901 tccgccgccg ggccgttttt ctcacgggcg ttcgtcggag gaggcgcggc atggcgcagt
      961 gggaccagat cgacgtcgat ggctcgccga tgcgcctgta cgtgggcatg ccgagcctcg
     1021 gacgggcatt ccccgcggtc atcgtcatcc agcacggccc cggggttgac cggttcatcc
     1081 aggaccgcgt cgagaccttg tcggagcagc acggctacct cgccatcgcg cccgagctct
     1141 accaccgaca gcccgaggac ggcggcgaca tgatgacccg catcggccgg ctgcgagatc
     1201 ccgaggtggt caacgacgtc agcgccgcgg tcaactacgc gcgccggctc aaggacaccc
     1261 agctcggcga cgtgggcatc atcggcttct gcatgggcgg ccgcgtcgcg tacctcatgg
     1321 cggcctccaa gccggtcttc aaggcggcgg gcgtgttcta cggcggcaac atcatgaaag
     1381 cgtggggcga cgggcccacg cccttcgatc tcacgcccta cgtgcactgt ccgatcgtgg
     1441 ggttcttcgg caaagaggac gccaatccct caccggccga cgtggaccgg ctctcgaccc
     1501 agctcgacaa gtaccgcaag gcccacgagt tccaccgcta cgatggcgcc ggtcacgcct
     1561 tcctcaactt cagcaacccg gtcacctacc gcgaggggcc cgcccgcgac gcgtggcaga
     1621 agctcatccc gttcctgcag cggcacctgc agtcgagccg ccagccgcgt cagcggctcg
     1681 cctagttcgc agcggtcaag gcgtcaggct ggcggcggga gtcgccgcgg agcgcgtcgg
     1741 ccgcggctcc agcgggtggg tccggagcag ctcgcgaaag acctggcacc agcggcagtc
     1801 cacgccgccg tccatcgtcg agcaggtgca ccggccctcg aactgattgg cgatcgccac
     1861 cgccctcgcg cgtaggttca tgaccggcct ccagtcgttg acgaaacgat agccccggcg
     1921 cgcgacgccc ggatggcacg ggcgtgaaga gtacgtgacg ccgggggagg ctcttaccgg
     1981 gcgggggggc cgccgaacgt ggcgtgcgcc gcggtccacc gcgcgagcag cgcggggtcg
     2041 ggctcctgcg cgatgccggg cacccgcgag gtggccacct cgccggggcc gctcagcacg
     2101 atctcgggct tgcccaggtc gtcggcgtag aagtaggacg aggggaagat gtcgcccggg
     2161 taggtgaagt tggccagggt cgccagctcc gcgcagatcg ccccgcccac cgcgctctcg
     2221 agcatgccgc ccacccagca cgggatgccg tgttcggcgc agcgcgactg gatcaggcgg
     2281 gactcggtga gcccgcccac gcgggccatc ttgatgttca ccacccggca gctctcgagc
     2341 gcgatggccg cctccacgtg agccaggctc tggacgctct cgtccaggca gaccggcgtc
     2401 ccgatctgcc gctgcagggc cgcgtggttc accaggctca tgccgtcgtc ggccagcggc
     2461 tgctcgatca tggcgagctt gaagcggtcg agcgagcgga agagcggggt gtcggccggc
     2521 gagtaggccg cgttgcagtc gatgtggatg gtcaggtccg ggaacgtccc gcgcaccgcc
     2581 tccaccatga agagatccca tcccgggcgg aacttgagct tcacccgcgg aaagcccgcg
     2641 tcgaccgccc cctggacctt cgccatgagg agatcgagcg agtcctggat cccgaagtcg
     2701 gcccccaccg ccacgcggtc ggcggtgccg cccagggcgc ggtgcagcgg cagcccgcgg
     2761 cgcttggcgt cgagcaccca ccaggtgatg tcgagcgcgg cccgcgcgaa ctggttgccc
     2821 ttgatgaagg cgagccggtc caggagatcc tgcgaggtct cgatgtcctg gcccacgatc
     2881 cgcggggcca tgtgctcgcg cagggtgtgg aaggtggcga gggtgtgctc ggcggagtag
     2941 gccgggatgt agggggggca gctctcgccc cacgcgtagt gcccgccgcc ttccatgcgc
     3001 acgaggatgg tgtcggtgaa ggactggtcg gcgtacgagg tcttccacac gaaggccagc
     3061 ggcagcttca tccagtagac gtcgatccgg tcgatcttca agtcgcttcg ctcctacttg
     3121 gcccgcgcca cgatgaagag gcgccggaac gggaagaggg tgcggccgtc tgcgcgcggc
     3181 gggtaggccc gggccaccag cgccgcgtag gaagcctcga agcgggagcg ctcgggttcc
     3241 gcgagggcat ccagcagcgg cttgagccag gttcccttcg tccactcctt caccgggtcg
     3301 cggccctcga gcacctggag gtactcggtc tcccagatgt cgagcgcggc cgcgtgcgga
     3361 gcgagcacgc cgtagtagaa gtccgggtcg gccaccgggg ccggccgcag cagcggctcg
     3421 agggtggcgc gccaggggcc ggccagagcc gcctcggaga tcgcggtgtg cgagcgggcg
     3481 ccgaagttgc gcggcatctg caccgcgagc acgccgcccg gggcgaggcc acccagcagc
     3541 gtgctgaaga ggcgctcgtg gccgggcacc cagtgcagcg cggcgttgga gtagatcagg
     3601 tcggccggct gggccgggcg ccacgtggcc aggtcggcct gccgccaggt gatggagggc
     3661 gcggtggccg cggccttggc gagcatggcc tccgagtcgt ccacgccggt cacccgcgcg
     3721 tcgggccagc gctccttgat gaggcgggtc acgttggcgg cccccgcccc gagatcgtag
     3781 atctctcccg ggttgtcgag atccacccgg ttcaggagat cgacggccgg ccggagccga
     3841 tggtcggcga acttcagata ctgagccgga tcccacgcca tgcccgagag aataccgcaa
     3901 tcgccgcttc ccgggtcgcc ttgaccctct cgcgcgtcct ctggtataca atcgaccacc
     3961 cttgagccag accacggatt cgcgcctcgg ccgcgac
//
LOCUS       DATMCV010001002        12540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_321517, whole genome shotgun sequence.
ACCESSION   DATMCV010001002 DATMCV010000000
VERSION     DATMCV010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321517"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1132
                     /locus_tag="VNU03_22960"
     CDS             152..1132
                     /locus_tag="VNU03_22960"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77402.1"
                     /translation="MSPAAPPRRVLAWSVPVLAALIVLPFVVPPYQTVLLSYGLVLAI
                     AALGFNLLLGYTGLLSFGHSAYFGVGAYAVAFIVKYMKLESMEAFLVGGVLASGAVAA
                     IFGLLCVRYTKIFFGILTMALSQVLWSLAFKFFWVTGGSDGLRVPTPTLLGGLVRAGN
                     DKFEFLSHRYYFYVLAVFVVAVAVMWVIVHSPFGRALQAIRDNETRAEFVGVQVWHYR
                     WVAFLISGMFTGLAGALWVPLNGLTTPDILHWTFSGKMVFFTVLGGFQTFFGPIVGAI
                     IFNYLETYAVGHTVYWQMVLGIVLVVLVLALPAGIVGTLRRLLPRPARAV"
     gene            1129..1887
                     /locus_tag="VNU03_22965"
     CDS             1129..1887
                     /locus_tag="VNU03_22965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77403.1"
                     /translation="MSVDLLKTTQLTKSFGDTHAVDHVDFTVAAGEVLALIGSNGAGK
                     TTLINLISGLIAPDSGRVDFQGTDITRVSTHRKIALGIARSFQLVNLFDQLSVQDNVT
                     LAIFSREGRTRRLFSLADADTAVRDEGAAVLKQFGLDGRAGEPAGGLSQGERKLLDVA
                     VAYALRPKLLFLDEPTSGVSTREKAPIMDIITQVVRSGGITAVIVEHDMDVVFTYCPR
                     IVAMHQGTILADGTPEQIRSNPQVTANMLGTWTG"
     gene            1891..2613
                     /locus_tag="VNU03_22970"
     CDS             1891..2613
                     /locus_tag="VNU03_22970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77404.1"
                     /translation="MLDVERLNTYRGPAHVLHNLSLTVGDRESVCLVGRNGAGKTTTI
                     DSIMGLLPVKSGRIGFAGQDITRLPTHKRALAGIGYAPEDAGIFPDLTVAENFVISQS
                     LARSAGKGGGPVSADNGIDERVLGLFPEIRAFTARRGLFLSGGQKKMVAIGRAMTLSP
                     TILLLDEPFEGLAPVVVSRFIEAVKQIKGMGISLLIAESNLMTASRVADRLFAIDRGE
                     IIFEGEPGRAFENPDVMKTIRG"
     gene            2613..2960
                     /locus_tag="VNU03_22975"
     CDS             2613..2960
                     /locus_tag="VNU03_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77405.1"
                     /translation="MLAFLAPTPTPGRAAAILRDIFGGLPASFAFRLWDGQEVRVGPG
                     APVCTAVIKSPQIFLDLIRDPSPYNFAEAYVHSRLDFEGDLFQAMDVANAVETVKVTP
                     MRKLKIILSLWRR"
     gene            2960..4084
                     /locus_tag="VNU03_22980"
     CDS             2960..4084
                     /locus_tag="VNU03_22980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP77406.1"
                     /translation="MDHDAIVVGGGPGGSTAAWRLALAGLRPLVLDAAVFPRVKICAG
                     WVTPEALADLEVDPDKYPLTIQPFDQCVLEFEGARHQTDWRRPSSYGIIRREFDHYLL
                     ERAAAAGADVRWGIRVTSVTAQADRVRVETDHGAFEAPVVIGAGGHRCPVARALGQVS
                     EREEVVVAQESETRLPPEWAQRLEPFLRAPELHVEPDLRGYGWYFPKRDFLNVGVGCT
                     HGDDGSLPRRRDALMASLRASGRLPEGLPLAPFKGHAYVVRRQAPRRLHGARFCLIGD
                     AAGLARDLSGEGIGPAIKSGRLAAEAVAALVRHGRPLDGYARQIVERYGPGELGWLQR
                     RLSSLPEAVARLGVRIVLGSARARRRIVFNSIFGMREVAP"
     gene            4081..4992
                     /locus_tag="VNU03_22985"
     CDS             4081..4992
                     /locus_tag="VNU03_22985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HWP77407.1"
                     /translation="MTRFARPRSKQYDAEAIAHHYDVSNEFYALFLDPLMVYTCAYYR
                     DPDGKLEQAQADKLDLVCRKLRLKPGERVLDIGCGWGGFSIWAAQHYGVQAHGVTLSR
                     QQAEWATARIKREGLEGRCLVEYRDVRDLPADSRYDKIAAIGVIEHVGIPNYPAFFGR
                     VKEMLTDGGLYLNHGIHHEFHWQQTSQTDFLYRYVFPNGDLSGVTETLTEMERAGWEL
                     LDLENLRLHYARTCRHWAERLKARADEAREIVGEQVYRTWLLYLTCSSVAFATGSIGL
                     YQVLMQKQWDQTRGPDPTIREGIYAGW"
     gene            complement(5014..5472)
                     /locus_tag="VNU03_22990"
     CDS             complement(5014..5472)
                     /locus_tag="VNU03_22990"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HWP77408.1"
                     /translation="MATDRSYVAENQAQLARLRALVDSLTDAELAQPMPADWTVAGVL
                     AHLAYWDQRIVVLMDRWGADGRGTPPPPIDEAAVEWVNDAGKPLCLALPPRAAARLAV
                     EAATAADQRAAGASDALLAANAAAGSPLSMRRAEHRREHLDEIERVRARR"
     gene            5592..7121
                     /locus_tag="VNU03_22995"
     CDS             5592..7121
                     /locus_tag="VNU03_22995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP77409.1"
                     /translation="MDVLAAHAARHPDKPALVEGERVWSWAEFIGRRNRLAHGLLSLG
                     LPAGGHVIVYAENSLEHYLAGAAARAAGLIPAPMNHRLVAEEVTYILDHSDAVAVLVS
                     DRFVPMVEAVRPAASKVRRFVLLGAERRPWAEHLDDLLASGRTDPVELPAGGGFGASM
                     IYTGGTTGRPKGALRRGSNPQDLMDTLRAMDLLDPAHVHLVAGPMYHSAPGGLALYAH
                     LVGATVVIMPKFDPEQALAQIERHRCSSTFMAPTLLKRIVDLPPAVRARYDVSSMRAI
                     IMAAAPCPMSVKEGVVAYFGPALYEFYGSSELGVNTILRPEDVLRKPGSCGRAAPGKE
                     IALLDDDGRPVPVGEPGELHVRRCPGLLDEYYRDPEATARMQRGEWYTVGDVAYADAD
                     GFYYICDRKRDMIISAGVNIYPAEIEDVLHRLPGVLDAAVFGVPDDEWGERVHAALHV
                     RPGATLSREEITAFCREHMAGYKVPREISFHDVFPRDAAGKLLKRQLREPYWAGRAAR
                     V"
     gene            7124..8725
                     /locus_tag="VNU03_23000"
     CDS             7124..8725
                     /locus_tag="VNU03_23000"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP77410.1"
                     /translation="MRRFRARLTAKITVVIVLVLVIGFGVSTILTIQRESALLVEQNK
                     MAARQLIGTLVASIETAMLSERPDITRGLIDDLRSSKLVTGLVVYRRNGVEAFTDLAT
                     LREVSKEAELPKGVMASIEKMRREPGRTMAGPLFTRALETLQTQEALESRDGATTFTL
                     YQPIPNQERCQGCHGSDHTVRAVIQVATSMEPVMAEVRLQRNRQIMIGLMTILVAAVV
                     LAVALRQVVVRPIHALAVVARRIGEGDFAVRARAGAGDEVGELGGALNDMTEHLARTQ
                     KDLAARNTELATALENLQASRQRLDALEQLKGELSKFVPDAVKDLLERDPTATSLEKR
                     NEEVSVLFLDIAGYTRLSETVDAKRLNQLIQTYFSSFLEIIRHHHGDVNETAGDGLMV
                     IFQSKRRGESSGDEDHALNATRAAFAIRQRTAALNEEYAGVFPSVALHMGINTGQALV
                     GATKLGGAGSQRWTFTATGPTTNIAARFAGAAEGGDIVVGQVTADRIAGLFVLESLGE
                     RSFKNVSQPLAVYRIIPPGVYSKVT"
     gene            complement(8749..9165)
                     /locus_tag="VNU03_23005"
     CDS             complement(8749..9165)
                     /locus_tag="VNU03_23005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77411.1"
                     /translation="METPMKMPMKPLLLATTALLALGPASPVIPAREALALSAASSID
                     PRIRLDWEVGTGRAGRPVIQGYVYNDYGRPASDVHLLVETLDSSGAVIARNIGFVRGV
                     VHLYDRSYFEVPLKVSGVSYRVSVTSLDWKGGGGGL"
     gene            complement(9221..10438)
                     /locus_tag="VNU03_23010"
     CDS             complement(9221..10438)
                     /locus_tag="VNU03_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77412.1"
                     /translation="MSGDSGRWRALGLLSLAELLALSLWFSASAVLPALSREWALGDA
                     GRAGLTIAVQAGFIVGTLAAALTNLPDVVPARSLMMWGALAGAVFNAGLALGTDHLGP
                     ALVLRFLTGVCLAGTYPPAMKIAATWFREGRGLAIGLLVAALTVGSAAPHLIRGLTDL
                     PWRHTLLAASVLAAAGALTVTLVAEGPHRFPPARFDLRMATAVLRERGPRLACLGYFG
                     HMWELYAMWTWLGAFLAASLEARGGGSFAGLNASAATFACVGLAGGLGAYGGGALADR
                     WGRTTLTMAAMALSGLCALLIGFTFAGPPLATLLVALVWGVTVIADSAQFSTAVTELA
                     PAAYVGTALTTQTCLGFALTMASIWLIPPVVTMIGWRWAFGMLAIGPALGVVAMGRLR
                     ALPEAAAMAGGRR"
     gene            10633..11643
                     /locus_tag="VNU03_23015"
     CDS             10633..11643
                     /locus_tag="VNU03_23015"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HWP77413.1"
                     /translation="MTLSEHLARQQASLSAGSGASAPNVATVVGRIGRVAAVIAQELA
                     HAALRDQLGYAGGSNVTGDQVKKLDVWGHEVMVAALRETRVCAAVISEEASEPVEMPI
                     TMGDAGGANALVVCTDPVDGSSNLDVNGSVGTIFALRASEGKTPAGPAALGPGTRQLA
                     AGYVMYGPATTMVYTLGQGTHGFTLNPESGEFLLTHPDIRIPRRGKTYGINEGNVHSW
                     HPGQRAFVEHLKTPDKPSGRPYSLRYSGAMVADVHRTLLDGGLFMYPADWSDPKKPKA
                     KLRLLYEVAPMGMIVEQAGGRASTGLERVLDLVATEYHQRAAVILGSPEDVAMADEFY
                     RR"
     gene            11672..>12540
                     /locus_tag="VNU03_23020"
     CDS             11672..>12540
                     /locus_tag="VNU03_23020"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWP77414.1"
                     /translation="MESRVKEILSWYSSDSPGTRTNIARLLNHGRLAGTGKLVILPVD
                     QGFEHGPARSFAVNPPGYDPLYHFRLAIDAGCNAYAAPLGFLEAGAAEFAGEVPMILK
                     MNNHDVLHDEKDPLSAVTGSVKDALRLGCVAVGFTIYPGSSQAQIMYQQCREMIQEAK
                     ASGLAAVVWSYPRGTDVSKAGETGLDVVAYAAQIAAQLGAHIIKVKPPTEHSEQAEAK
                     KAYEKAKVPMATLSERVRHVVQSSFNGRRIVIFSGGATKENDESFFDECRGIRDGGAF
                     GSIIGRNSFQRPR"
BASE COUNT         1860 a         4470 c         4431 g         1779 t
ORIGIN      
        1 gcagcctcga gggcgcgctg atcggggccc tcatggtggg gctcgtgcgc gaggcgggga
       61 tcacgttctt tcccgagatc gagctggccg tgctctacct gatggcggcg gtggtgctcc
      121 tcatccggcc ggccgggatc ctggggcgcg cgtgagcccg gctgcgccgc cgcgccgggt
      181 gctcgcctgg tcggtgccgg tcctggccgc gctgatcgtg ctgccgttcg tggtgccgcc
      241 ctaccagacg gtgctgctct cctacgggct cgtgctcgcg atcgccgcgc tcggcttcaa
      301 cctcctcctg ggctacaccg gcctgctgtc gttcggccac tccgcgtact tcggggtggg
      361 ggcctacgcg gtggccttca tcgtgaagta catgaagctc gagtcgatgg aggccttcct
      421 ggtgggcggc gtcctggcct ccggcgcggt ggcggcgatc ttcgggctgc tctgcgtgcg
      481 ctacaccaag atcttcttcg gcatcctcac catggcgctg tcgcaggtcc tctggagcct
      541 cgcgttcaag ttcttctggg tcaccggggg cagcgacggg ctccgggtgc cgaccccgac
      601 cctgctgggt gggctcgtgc gggcgggcaa cgacaagttc gagttcctct cccaccgcta
      661 ctacttctac gtgctcgcgg tcttcgtggt ggcggtggcg gtcatgtggg tgatcgtgca
      721 ctccccgttc ggccgggcgc tccaggccat ccgcgacaac gagacccgcg ccgagttcgt
      781 cggggtgcag gtctggcact accgctgggt ggccttcctg atctcgggca tgttcaccgg
      841 cctcgccggg gcactctggg tgccgctcaa cggcctcacc actcccgaca tcctccactg
      901 gaccttctcg ggcaagatgg tcttcttcac ggtgctgggc ggcttccaga ccttcttcgg
      961 ccccatcgtc ggcgcgatca tcttcaacta cctcgagacg tacgcggtcg gccacaccgt
     1021 gtactggcag atggtgctcg gcatcgtgct ggtggtgctg gtcctggccc ttcccgccgg
     1081 catcgtgggc acgctgaggc gcctgctccc gcggccggcg cgggcggtgt gagcgtggac
     1141 ctcctcaaga ccacccagct caccaagtcc ttcggggaca cccacgcggt ggaccacgtc
     1201 gacttcacgg tggcggcggg cgaggtgctc gccctcatcg gctcgaacgg ggcggggaag
     1261 accaccctga tcaacctgat cagcgggctg atcgcgcccg actcgggccg ggtcgacttc
     1321 caggggaccg acatcacgcg ggtctcgacc caccgcaaga tcgcgctcgg catcgcgcgc
     1381 agcttccagc tcgtgaacct cttcgaccag ctctcggtcc aggacaacgt gaccctcgcg
     1441 atcttctccc gcgagggccg cacccggcgc ctgttctcgc tcgccgacgc cgacaccgcg
     1501 gtgcgggacg agggcgcggc ggtgctgaag cagttcggcc tggacggccg cgcgggcgag
     1561 ccggccgggg ggctgtccca gggggagcgc aagctcctgg acgtggcggt ggcctacgcg
     1621 ctgcggccca agctgctctt cctcgacgag ccgaccagcg gggtgagcac gcgggagaag
     1681 gccccgatca tggacatcat cacccaggtg gtgcgctccg gcggcatcac cgcggtgatc
     1741 gtcgagcacg acatggacgt ggtgttcacc tactgcccgc gcatcgtggc gatgcaccag
     1801 ggcacgatcc tggccgacgg caccccggag cagatccgga gcaacccgca ggtcaccgcc
     1861 aacatgctcg gcacctggac gggctgagcc atgctcgacg tcgagcgcct caacacctat
     1921 cgcgggcccg cccacgtgct ccacaacctg tcgctcacgg tgggggaccg ggagtcggtg
     1981 tgcctggtcg gccgcaacgg ggcgggcaag accaccacca tcgacagcat catgggcctc
     2041 ctgccggtca agagcggccg catcgggttc gcgggccagg acatcacccg gcttccgacc
     2101 cacaagcgcg ccctcgccgg gatcgggtat gctccggagg acgcggggat cttcccggat
     2161 ctgacggtgg ccgagaactt cgtgatcagc cagtcgctgg cccggagcgc ggggaagggg
     2221 ggcgggccgg tgtcggccga caacggcatc gacgagcggg tgctggggct gttcccggag
     2281 atccgggcct tcaccgcccg acgcggcctc tttctctcgg gaggacagaa gaagatggtg
     2341 gcgatcgggc gggccatgac gttgtcgccg acgatcctcc tgctggacga gcccttcgag
     2401 gggctggctc cggtggtggt gagccgcttc atcgaggcgg tgaagcagat caagggcatg
     2461 ggcatctcgc tgctgatcgc ggagtcgaac ctcatgaccg cgtcccgcgt cgcggaccgc
     2521 ctgttcgcga tcgaccgcgg cgagatcatc ttcgagggcg agcccggccg ggccttcgag
     2581 aaccccgacg tcatgaagac gatccgcggc tgatgctcgc cttcctcgcc ccgactccga
     2641 cccccggccg cgcggccgcc atcctgcgcg acatcttcgg cggcctgccc gcgtccttcg
     2701 cgttccgcct ctgggacggg caggaggtcc gggtggggcc gggggccccg gtgtgcaccg
     2761 cggtcatcaa gagcccgcag atctttctcg atctcatccg cgatccctcg ccgtacaatt
     2821 tcgccgaagc gtacgtgcac agccggctcg acttcgaggg cgacctcttc caggccatgg
     2881 acgtggcgaa cgcggtggag acggtgaagg tgaccccgat gcggaagctc aagatcatcc
     2941 tgtccctctg gagacggtga tggaccacga cgcgatcgtg gtcggcggcg gcccgggggg
     3001 cagcaccgcg gcctggcgcc tggccctggc cggactgcgg ccgctggtgc tcgacgcggc
     3061 ggtgttcccg cgcgtgaaga tctgcgcggg gtgggtgacg ccggaggcgc tcgccgacct
     3121 cgaggtcgat cccgacaagt atcccctcac gatccagccc ttcgaccagt gcgtgctcga
     3181 gttcgagggg gcccgccacc agaccgactg gcgccgtccc tccagctacg gcatcatccg
     3241 ccgcgagttc gaccactacc tgctcgagcg ggccgccgcc gcgggggcgg acgtgcgctg
     3301 ggggatccgc gtcacctccg tcaccgcaca ggccgaccgg gtccgcgtgg agaccgacca
     3361 cggcgcgttc gaggcgccgg tggtgatcgg ggcgggcggc catcgctgcc cggtggcgcg
     3421 cgcgctcggc caggtgtcgg agcgcgaaga ggtggtggtg gcccaggaga gcgagacgcg
     3481 gcttccgccc gagtgggcgc agcgcctcga gcccttcctg cgtgcccccg agcttcacgt
     3541 ggagcccgac ctgcggggat acggctggta cttcccgaag cgcgacttcc tcaacgtggg
     3601 ggtgggctgc acccacggcg acgacggcag cctgccccgc cggcgggacg cgctcatggc
     3661 gagcctgcgc gcctccgggc ggctgcccga ggggctgccg ctcgccccgt tcaagggcca
     3721 cgcctacgtg gtgcgccgcc aggcgccgcg ccgcctccac ggcgcgcggt tctgcctgat
     3781 cggcgacgcg gccgggctcg ctcgcgacct gtccggggag ggcatcggcc ccgcgatcaa
     3841 aagcgggcgg ctggccgcgg aggcggtcgc ggcgctcgtc cgccacgggc gtccgctcga
     3901 cggctacgcc cggcagatcg tcgagcgcta cggcccggga gagctcggct ggctgcagcg
     3961 gcggctctcg agcctgcccg aggcggtggc ccgcctcggg gtccgcatcg tgctgggctc
     4021 cgcgcgagcc cgtcgtcgca tcgtgttcaa ctccatcttc ggtatgcggg aggtggcccc
     4081 atgacccggt tcgcacgtcc ccggtcgaag cagtacgacg ccgaggccat cgcgcaccac
     4141 tacgacgtct ccaacgaatt ctacgcgctc ttcctcgacc cgctgatggt ctacacctgc
     4201 gcgtactacc gggatccgga cggcaagctc gagcaggcgc aggcggacaa gctcgacctg
     4261 gtctgccgca agctccgcct gaagccgggg gagcgggtgc tcgacatcgg ctgcggctgg
     4321 ggcggcttca gcatctgggc cgcccagcat tacggagtcc aggcgcacgg ggtcaccctg
     4381 tcgcgccagc aggcagagtg ggcgaccgcg cgcatcaagc gcgaggggct cgaggggcgc
     4441 tgcctcgtcg agtatcgcga cgtgcgcgac ctcccggccg attcgcgcta cgacaagatc
     4501 gccgcgatcg gcgtcatcga gcacgtgggc attcccaact acccggcctt cttcggccgg
     4561 gtgaaggaga tgctcaccga cggtgggctc tacctcaacc acggcatcca ccacgagttc
     4621 cactggcagc agaccagcca gaccgacttc ctctaccgct acgtgttccc caacggcgac
     4681 ctctccgggg tgaccgagac gctcaccgag atggagcgcg cgggctggga gctgctcgat
     4741 ctggagaacc tccgcctgca ctacgcgcgc acctgccggc actgggcgga gcgcctgaag
     4801 gcccgcgccg acgaggcgcg cgagatcgtg ggcgagcagg tctaccgcac gtggctcctc
     4861 tacctcacgt gctcgtcggt ggccttcgcg accgggtcca tcgggctcta ccaggtgctg
     4921 atgcagaagc agtgggacca gacgcgcggg cccgatccca cgatccgcga gggaatctac
     4981 gccggctggt aggcccgcgg gcgggcgctc cgcctagcgc cgggcgcgga cccgctcgat
     5041 ctcgtccagg tgctcgcggc ggtgctcggc gcgccgcatg ctgagcgggg agccggccgc
     5101 cgcgttggcg gcgagcagtg cgtccgaggc gcccgccgcg cgctggtccg ccgcggtcgc
     5161 ggcctcgacg gcgaggcggg cggcggcccg gggcgggagc gccaggcaga gcggcttgcc
     5221 cgcgtcgttg acccattcga ccgcggcctc gtcaatgggc ggaggcgggg tgccccggcc
     5281 gtccgcgccc cagcggtcca tcagcaccac gatccgctga tcccagtagg ccaggtgggc
     5341 cagcacgccg gccaccgtcc agtcggccgg catcggctgc gccagctccg cgtcggtcag
     5401 cgagtcgacg agggcccgca gccgcgcgag ctgggcctgg ttctccgcca cgtacgatcg
     5461 atccgtcgcc atgtctctcc tcctgtcgcg tgcgttgacc ggctcagact gccacaaccc
     5521 cgctctgcgg gcgacgtgaa acgccgaccc gttgccgccc ccgcccgaac tgtgctacac
     5581 acgcgggagc gatggacgtc ctcgccgcgc acgcggcccg acaccccgac aagcccgctc
     5641 tcgtcgaagg ggagcgggtc tggagctggg ccgagttcat cggtcggcgt aaccggctcg
     5701 cccacggcct cctctcgctc gggctcccag cgggtggcca cgtcatcgtc tacgccgaga
     5761 actccctcga gcactacctg gccggggcgg cggcgcgagc cgccgggctc atccccgcgc
     5821 ccatgaacca ccggctggtc gccgaggagg tcacctacat cctcgaccac tcggacgcgg
     5881 tggcggtgct ggtgagcgat cgcttcgtgc ccatggtgga ggcggtccgg cccgccgcct
     5941 ccaaggtgcg gcgcttcgtc ctcctgggcg cggagcgccg cccgtgggcc gagcacctcg
     6001 acgacctgct ggcgagcggc cggaccgatc cggtcgagct gccggccggc ggcgggttcg
     6061 gcgcgtcgat gatctacacc ggcggcacca ccgggcggcc caagggcgcc ctccgccggg
     6121 gctcgaatcc gcaagatctg atggacacgc tccgggcgat ggacctgctc gacccggccc
     6181 acgtccacct ggtggccggg ccgatgtacc actcggcgcc gggcggcctc gcgctgtacg
     6241 cccacctggt cggggccacc gtggtgatca tgccgaagtt cgaccccgag caggcgctgg
     6301 cccagatcga gcgccaccgc tgcagcagca cgttcatggc cccgaccctg ctcaagcgca
     6361 tcgtggatct gccgcccgcg gtgcgcgcgc gctacgacgt gtcctcgatg cgcgcgatca
     6421 tcatggcggc cgcgccctgc ccgatgagcg tgaaggaggg cgtggtggcc tacttcgggc
     6481 ccgccctcta cgagttctac ggctcgagcg agctcggggt gaacacgatc ctgcgcccgg
     6541 aggacgtgct ccggaagccc ggctcgtgcg gccgggccgc gcccggcaag gagatcgccc
     6601 tgctcgacga cgacggccgg ccggttccgg tgggcgagcc gggcgagctc cacgtgcggc
     6661 gctgtcccgg gctgctcgac gagtactacc gcgatccgga ggcgaccgcg cggatgcagc
     6721 ggggggagtg gtacaccgtc ggcgacgtgg cctacgcgga cgcggacggg ttctactaca
     6781 tctgcgaccg caagcgcgac atgatcatct cggcgggcgt gaacatctat ccggccgaga
     6841 tcgaggacgt gctgcatcgc ctccccgggg tgctcgacgc ggcggtcttc ggggtgcccg
     6901 acgacgagtg gggcgagcgg gtgcacgcgg ccctgcacgt gcggccgggg gcgacgctct
     6961 cccgcgagga gatcaccgcc ttctgccggg agcacatggc gggctacaag gtgccccgcg
     7021 agatctcctt ccacgacgtc ttcccgcgcg acgcggcggg caagctgctc aagcgtcagc
     7081 tccgcgagcc ctactgggcg ggccgcgccg cgcgggtgtg acgatgcggc gcttccgcgc
     7141 gcggctcacc gccaagatca cggtggtgat cgtcctggtg ctggtcatcg ggttcggagt
     7201 ctccaccatc ctgaccatcc agcgggagag cgcgctcctg gtcgagcaga acaagatggc
     7261 ggcccggcag ctgatcggca ccctggtggc gagcatcgag accgcgatgc tctccgagcg
     7321 gcccgacatc acgcgcggcc tcatcgacga cctgcgcagc tcgaagctcg tcaccggcct
     7381 cgtggtctac cggcgcaacg gggtggaggc gttcacggat ctggccaccc tgcgcgaggt
     7441 ctcgaaggag gcggagctgc ccaagggggt catggcctcc atcgagaaga tgcgccggga
     7501 gccgggccgc accatggccg ggccgctctt cacccgcgcg ctcgagaccc tgcagaccca
     7561 ggaggcgctc gagtcgcggg acggcgcgac gaccttcacc ctctaccagc cgatccccaa
     7621 ccaggagcgc tgccagggct gccacggctc ggaccacacg gtgcgcgcgg tcatccaggt
     7681 ggccacctcg atggagccgg tcatggcgga ggtgcggctg cagcgcaatc gccagatcat
     7741 gatcggcctg atgacgatcc tggtcgcggc ggtggtgctg gcggtggcgc tgcgccaggt
     7801 ggtcgtgcgc ccgatccacg cgctcgcggt ggtggcccgc cgcatcgggg aaggcgactt
     7861 cgcggtgcgg gcccgggccg gcgcgggcga cgaggtgggc gagctgggcg gcgcgctgaa
     7921 cgacatgacc gagcacctgg cccggaccca gaaggacctg gccgcccgca acaccgagct
     7981 ggccaccgcg ctcgagaacc tgcaggcctc ccggcagcgg ctcgatgcgc tcgagcagct
     8041 gaagggggag ctctccaagt tcgtgcccga cgcggtcaag gacctgctcg agcgggaccc
     8101 caccgcgacc agcctcgaga agcgcaacga ggaggtctcg gtgctcttcc tcgacatcgc
     8161 gggctacacg cggctcagcg agaccgtcga cgccaagcgg ctgaaccagc tgatccagac
     8221 ctacttctcg agcttcctcg agatcatccg ccaccaccac ggcgacgtga acgagaccgc
     8281 gggcgacggg ctgatggtca tcttccagag caagcgccgg ggcgagagca gcggtgacga
     8341 ggaccacgcc ctcaacgcca cccgggccgc cttcgcgatc cgccagagga ccgccgcgct
     8401 caacgaggag tacgcggggg tgttcccttc ggtggccttg cacatgggga tcaacaccgg
     8461 gcaggcccta gtgggcgcca ccaagctggg cggcgccggc tcgcagcgct ggaccttcac
     8521 cgctaccggc cccaccacca acatcgcggc ccgcttcgcc ggggccgccg agggcggcga
     8581 catcgtggtg ggccaggtga ccgccgaccg gatcgcgggc ctgttcgtcc tcgagtcgct
     8641 cggcgagcgc tccttcaaga acgtctcgca gccgctcgcg gtctaccgca tcatcccgcc
     8701 cggggtctac agcaaggtca cctgacccgg tgttgcggga ttcgccgcct acaggccgcc
     8761 gccaccgccc ttccagtcga gagaagtgac ggagacacgg taagatacgc cggacacctt
     8821 cagcggaacc tcgaagtagg atcgatcgta gaggtggacg acgccgcgca cgaacccgat
     8881 gttgcgggcg atgaccgcgc cggagctatc cagggtctcc accagcaggt gcacgtcgga
     8941 cgccggccgc ccgtagtcgt tgtagacgta gccctggatc accggtctcc cggcccgtcc
     9001 ggtcccgacc tcccagtcga gccggatcct cgggtcgatg ctcgacgccg cgctcagggc
     9061 caaggcctcg cgggccggga tgaccgggct cgcggggccc agcgcgagga gggccgtcgt
     9121 tgcgagcagc aggggcttca tcggcatctt catcggcgtc tccatggcgg gttgcctccc
     9181 gcccgtatga tgccccacgg gccggtcgag atgcggacga tcagcggcgg ccaccggcca
     9241 tggccgcggc ctcgggcagg gcccgcagcc ggcccatcgc caccaccccc agcgccggtc
     9301 cgatcgcgag catgccgaag gcccagcgcc agccgatcat cgtgaccacc ggcgggatca
     9361 gccagatgga ggccatggtc agagcgaagc ccaggcaggt ctgggtggtg agcgcggtgc
     9421 ccacgtaggc ggccggcgcc agctcggtca ccgcggtcga gaactgggcc gagtccgcga
     9481 tcaccgtcac cccccacacc agggccacca gcagcgtcgc caggggcggg ccggcgaagg
     9541 tgaagccgat cagcagcgcg cagaggccgg agagcgccat ggccgccatg gtgagcgtgg
     9601 tgcggcccca gcggtcggcg agggccccgc cgccgtaggc gcccagcccg ccggccaggc
     9661 ccacgcacgc gaaggtcgcc gcgctcgcgt tgaggcccgc gaagctgccg ccgccgcgcg
     9721 cctcgaggct cgcggccagg aaggcgccca gccaggtcca catcgcgtag agctcccaca
     9781 tgtgcccgaa gtagccgaga cacgccagcc ggggcccacg ctcgcggagg accgcggtgg
     9841 ccatgcgcag gtcgaaccgg gcgggaggga agcggtgggg cccttccgcg accagggtga
     9901 ccgtgagcgc gccggccgcc gccaggaccg aggctgcgag cagggtgtga cgccacggca
     9961 gatccgtgag gccgcggatg aggtgcgggg cggccgagcc caccgtgagc gcggcgacca
    10021 gcagaccgat ggcgaggccg cgcccctcgc ggaaccaggt ggccgcgatc ttcatcgcgg
    10081 gcggatacgt gccggccaga cagacgccgg tgaggaagcg caggacgaga gccgggccca
    10141 ggtggtcggt gccgagcgcg agcccggcgt tgaacaccgc tccggccagc gcgccccaca
    10201 tcatgagcga gcgcgcgggc acgacgtcgg ggaggttggt gagcgcggcg gcgagcgtgc
    10261 ccacgatgaa cccggcctgc accgcgatcg tgaggcccgc ccggccggcg tcaccgaggg
    10321 cccactcgcg cgagagcgcc ggcagcaccg cggaggcgct gaaccacagc gacagcgcga
    10381 ggagctcggc cagggacagc agcccgagcg ctctccaccg acccgagtcg ccgctcatga
    10441 tcggagcatc taaccatagt ccggccggga gcacgcagga gatgcgcaag ctgggacgcg
    10501 cccgactcac gtgcgtgcga tccgacgtcg ggtgtgttga ctgagcacaa ctgatcgtct
    10561 agcattctgt gaggcgcagc gcattcgcgc cacccgactc aacgacgaga ggtttccatg
    10621 gccgaatcgc cgatgacgct atcggaacat ctcgctcgcc agcaggcctc gctctccgcc
    10681 ggatccggcg cctcggcacc caacgtcgcc accgtggtgg gccgcatcgg ccgggtcgcc
    10741 gcggtcatcg cacaggagct ggcgcacgcg gcgctccgcg atcagctcgg ctacgcgggc
    10801 gggagcaacg tgaccggcga ccaggtcaag aagctcgacg tgtggggcca cgaagtcatg
    10861 gtggccgcgc tgcgcgagac gcgggtctgc gccgcggtga tctccgagga ggcgtccgag
    10921 ccggtcgaga tgcccatcac gatgggtgac gcgggcggcg cgaacgccct cgtggtgtgc
    10981 acggatccag tcgacggctc gtcgaacctc gacgtcaacg gctcggtggg cacgatcttc
    11041 gcgctccgcg catccgaagg gaagacgccg gcgggcccgg ctgcgctcgg ccccggcacc
    11101 cgacagctcg cggccgggta cgtcatgtac gggccagcca ccaccatggt ctacacgctc
    11161 gggcagggga cgcacggctt cacgctcaac ccggagagcg gcgagttcct gctcacccat
    11221 cccgacatcc gcattccccg ccgcggcaag acctacggca tcaacgaggg caacgtccac
    11281 tcgtggcatc ccggccagcg cgccttcgtc gagcatctca agacgcccga caagccgagc
    11341 ggccggccct actcgctccg ctactcgggc gcgatggtgg ccgacgtcca ccgtaccctg
    11401 ctggacggcg ggctcttcat gtatccggcg gactggagcg atccgaagaa gcccaaggcc
    11461 aagctccggc tgctctacga ggttgccccg atgggtatga tcgtggagca ggcgggcggg
    11521 cgggcgagca ccggcctcga gcgggtgctc gatcttgtgg ccaccgagta tcaccagcga
    11581 gcggccgtca tcctgggcag ccccgaggac gtggcgatgg ccgacgagtt ctaccgtcga
    11641 taaccgcaac cggacagaaa ggggcacgcc aatggaatcg cgagtgaagg agatcctgag
    11701 ctggtacagc agcgacagcc cgggcacgcg aaccaacatc gcccgcctgc tcaaccacgg
    11761 gcgcctggcc ggcaccggca agctggtgat cctgccggtg gaccagggct tcgagcacgg
    11821 gccggcgcgg agcttcgcgg tgaacccgcc cggctacgat cccctgtacc acttccgtct
    11881 cgccatcgac gccggctgca acgcctacgc ggcgccgctc gggttcctgg aagcgggggc
    11941 cgccgagttt gcgggcgagg tgccgatgat cctcaagatg aacaaccacg acgtgctcca
    12001 cgacgagaag gacccgctct ccgcggtgac cggctcggtc aaggacgcgc tccggctcgg
    12061 ctgcgtcgcg gtgggcttca cgatctaccc gggctcctcg caggcccaga tcatgtacca
    12121 gcagtgccgc gagatgatcc aggaggccaa ggccagcggc ctcgccgcgg tggtgtggtc
    12181 ctaccctcga ggcactgacg tgagcaaggc gggggagacc ggtctggacg tcgtcgccta
    12241 cgcggcccag atcgcggccc agctcggagc ccatatcatc aaggtgaagc cgcccaccga
    12301 gcacagcgag caggccgagg ccaagaaggc ctacgagaag gccaaggtgc cgatggccac
    12361 actcagcgag cgggtgcgcc acgtggtgca gtcctccttc aacggccgcc gcatcgtgat
    12421 cttctcgggt ggggccacca aggagaacga cgagtcgttc ttcgacgagt gccgaggcat
    12481 ccgcgacggt ggggctttcg gctccatcat cgggcgcaac tcgttccagc ggccgcgcga
//
LOCUS       DATMCV010001003         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_188612, whole genome shotgun sequence.
ACCESSION   DATMCV010001003 DATMCV010000000
VERSION     DATMCV010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188612"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VNU03_23025"
     CDS             complement(<1..1160)
                     /locus_tag="VNU03_23025"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP77415.1"
                     /translation="MKIGRLFPDLLATLGGVASSEAGFARTLKRLVVLSGARAGALRF
                     RPGEAAPVDLVVGTRPGSALDRWLRARLDPPRRGVRLEKPTATPPGSKGRGQVALATA
                     LGDPAHPMGALVLLGPGGARGLTRETLPPSFPREFGHALEHVWRLRQRTIRLEVINQI
                     TALTGTTLSLERVYEKVAEAVGRLIRFDALVVTLLDRERGEFRVLDVAARTTMPETFD
                     AGIPIAGTLTAWVASQQAPRRIDDATIDPSIPTVSRELLSRRGFRSVILAPLLSQGQA
                     IGTLNVTHREPGAFTDADIEVLTEVARPLAAAIEHSRLHAESTQRAEELAALNRTSQL
                     ITARLDLPSVLETISRSVTALMGSTGCGIGLLSPDRTTIEQAAAHGFRTLEWR"
     gene            complement(1168..>2079)
                     /locus_tag="VNU03_23030"
     CDS             complement(1168..>2079)
                     /locus_tag="VNU03_23030"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP77416.1"
                     /translation="PGGSSSGPAAAVAARMVPLALGSQTVGSVLRPAAYCGVVGLKPT
                     HGRISVAGVLALAESLDHVGVFARCVEDCALALSLLAGPDPADPHSSPRPPEPYLAAL
                     AAPAPPRIGVLAGLVAQATPEMAKHLDAIMRGLEGAGARLVDVDLPDSFPMLRRAGET
                     VLAVEAAHAHAPLYAAHAADYPPRIRELIEQGHAVSALQYLAAQDARRHARDDLDAVA
                     TRHDVLLAPTIGAPAPRGLDSTGDPGFCAPFTFAGLPAISLPTGVDPSGLPLALQLIG
                     PAWGEARLLAASAWCERVIRFDAGPSL"
BASE COUNT          282 a          753 c          813 g          231 t
ORIGIN      
        1 cgccattcca gcgtgcggaa gccgtgcgcg gcggcctgct cgatcgtggt ccggtccggg
       61 ctcagcaggc cgatgccgca gccggtgctg cccatgagcg cggtgacgga gcggctgatg
      121 gtctcgagca ccgagggcag gtcgaggcgc gcggtgatga gctggctcgt gcgattgagg
      181 gcggcgagct cctccgcgcg ctgggtggac tccgcgtgca ggcgcgagtg ctctatcgcc
      241 gcggccagcg gtcgcgccac ctcggtgagc acctcgatat cggcgtcggt gaaggcgccc
      301 ggctcccggt gggtgacgtt gagggtgccg atcgcctggc cctgcgacag gagcggcgcc
      361 aggatcacgg atcggaagcc ccgccgcgag agcagctcgc ggctcacggt ggggatggac
      421 ggatcgatgg tggcgtcgtc gatgcggcgg ggcgcctgct ggctcgccac ccacgcggtc
      481 agggtgcccg cgatggggat cccggcgtcg aaggtctcgg gcatcgtggt acgggccgcc
      541 acgtcgagca cgcggaactc gccgcgctcg cggtcgagca gcgtcactac gagcgcgtcg
      601 aagcggatga gacgccccac cgcttccgcg accttctcgt agacccgctc gagcgagagc
      661 gtggtcccgg tgagcgcggt gatctggttg atgacctcga gccggatggt gcgctgccgc
      721 agccgccaga cgtgctcgag ggcgtggccg aactcgcggg gaaaactcgg cggcaacgtc
      781 tcccgggtga ggccgcgcgc cccgcccggc ccgaggagga cgagggcgcc catcggatgc
      841 gccgggtcac cgagggcggt ggcgagcgcc acctggcccc gccccttcga tccgggcggc
      901 gtcgcggtcg gcttttcgag ccgcacccct cgccgcggcg gatccagacg ggcgcggagc
      961 cagcggtcga gcgccgagcc gggccgcgtg cccaccacga gatccaccgg agcggcctcg
     1021 cccggccgga agcgcagcgc gcccgcgcgg gcccccgaga gcaccaccag ccgcttcagc
     1081 gtccgggcga agcccgcctc cgaggaggcc acgcccccca gggtggccag caggtcggga
     1141 aagagccgcc cgatcttcac gggcggctca gagcgacggc cccgcgtcga agcggatcac
     1201 gcgctcgcac caggcggagg ccgccagcag ccgcgcctcg ccccacgcag gaccgatgag
     1261 ctgcagcgcc agcggcagac ccgacggatc cacgccggtc ggcagcgaga tcgcggggag
     1321 tccggcgaag gtgaacggcg cgcagaagcc cgggtcgccg gtggagtcga ggccccgcgg
     1381 cgcgggggcg ccgatggtgg gggcgagcag cacgtcgtgg cgcgtggcca ccgcgtccag
     1441 gtcgtcgcgc gcgtggcggc gggcgtcctg ggccgccagg tactgcagcg cggagaccgc
     1501 gtggccctgc tcgatcagct ccctgatgcg aggcggatag tcggccgcgt gcgcggcgta
     1561 gagcggggcg tgcgcgtgcg cggcctcgac cgccaacacc gtctcgcccg cgcggcggag
     1621 cattgggaac gagtcaggca gatccacgtc gacgagcctc gcgcccgctc cctcgagccc
     1681 gcgcatgatg gcgtccaggt gcttcgccat ctcgggcgtc gcctgcgcca ccagcccggc
     1741 cagcaccccg atgcgcggcg gcgcgggcgc ggcgagcgcg gccagatagg gctcgggcgg
     1801 ccgcgggctc gagtgcggat cggccggatc gggcccggcc agcagcgaca gcgcgagcgc
     1861 gcagtcctcg acgcagcggg cgaagacccc gacgtggtcg aggctctcgg cgagggccag
     1921 cacacccgcc acgctgatcc ggccgtgggt cggcttgagg ccgaccaccc cgcagtaggc
     1981 ggcgggccgc agcaccgagc ccaccgtctg agagccgagc gccagcggca ccatgcgcgc
     2041 ggccaccgcc gccgccgggc cgctcgagga gccacccgg
//
LOCUS       DATMCV010001004         2987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_188643, whole genome shotgun sequence.
ACCESSION   DATMCV010001004 DATMCV010000000
VERSION     DATMCV010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188643"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VNU03_23035"
     CDS             complement(<1..184)
                     /locus_tag="VNU03_23035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWP77417.1"
                     /translation="MSCLLHLGLATGLVVGQHWVSAAIRPPVLPVQLVTLETQEEPPR
                     EAPPSPPAPRAPIRPPR"
     gene            complement(321..857)
                     /locus_tag="VNU03_23040"
     CDS             complement(321..857)
                     /locus_tag="VNU03_23040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77418.1"
                     /translation="MSANRTIVLTGVLLAAVALLAATGPAMAHSSASIIEPVASTAEA
                     SIPPVTLSPVPAVAAAADSSGFTPWIAAGALVLAGVALRRRPRRTLVLCLALLLTLFA
                     FENALHSVHHGLDPKKADACTIAAASAHLAAVAVDGIVETSLVLATAGHATEPDPSTL
                     PARWLGPDQGRAPPAPAA"
     gene            955..2265
                     /gene="rimO"
                     /locus_tag="VNU03_23045"
     CDS             955..2265
                     /gene="rimO"
                     /locus_tag="VNU03_23045"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWP77419.1"
                     /translation="MKVHFTTLGCPKNQVDSELMLGMLTEAGHEIAAAAEGADCLVVN
                     TCAFIDRAREESVNTILELAHLKERGRARALIVTGCLTQRYGLEISKEIPEVNAILGT
                     SELHRIVDLVNQADGRQAWVSAAPPGYLYDAQTPRLLTGRVPYAYVKIAEGCDMGCTF
                     CAIPQFRGKHRSRALTDIVAEVSGLARRGIQEAILVSQDTLAYGRDLPGNGDIGDLLL
                     ALSDTPMPWIRPMYLHPAHVTERLIEKWRRARVVPYLDMPVQHGDDAILRAMRRGVTA
                     QRMKDIVARLRDALPGVTLRTTLLVGFPGETEAAFQNLLAFVEDVAFDRLGVFTYSVE
                     EGTPAADMPDQVPAEVMAERAQQVQDLQDRLAWPRQKALFGTRQTVLVDGPSPDPAFP
                     FEGRTAGQAPEIDGVVLLRNARLTPGRFADVGIVEVDGYELVGE"
     gene            2300..2734
                     /locus_tag="VNU03_23050"
     CDS             2300..2734
                     /locus_tag="VNU03_23050"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HWP77420.1"
                     /translation="MASVFGAGYSPVASGTVGSFVTAVALWLLPFTPLRIAVTLVAVI
                     LIGIWAGGRVERVLGRKDPGVIVIDEVAGMLLSVIVLPRTLPVLVTAFLLFRLFDIWK
                     PFPARESQALTGGMGVMVDDLIAGLYALVLVMGARFLFGVPA"
     gene            2739..>2987
                     /locus_tag="VNU03_23055"
     CDS             2739..>2987
                     /locus_tag="VNU03_23055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77421.1"
                     /translation="MSQTWIVTVGAARLGAADDPAGLLVARALLADGVPVASRQIVDE
                     DETAVEEALRSAVEVASLVVVLAHPGGSSGEVVRRALSR"
BASE COUNT          410 a         1038 c         1114 g          425 t
ORIGIN      
        1 gcctcggggg gcggatcggc gcccgtggcg cgggcggcga cggaggcgcc tcgcgcgggg
       61 gctcctcctg ggtctccagc gtgaccagct gcaccggcag gaccggtggg cggatcgcgg
      121 cggacaccca gtgctgcccg acgacgaggc cggtcgccag ccccaggtgg agcaggcacg
      181 acaccgcgat cggcgttcct cgagaccgca acagctcgac caaatggagt actcccccta
      241 gcgcacaagt cagagcacga aatcttcggg acccgcgcgg cggcgtgagc cgcactggcg
      301 cgggacgagc ttcgggttgg ctaggcggcg ggagcgggag gggcccggcc ctggtcggga
      361 ccgagccagc gggcaggtag cgtcgacggg tcgggctcgg tggcgtgccc ggcggtggcg
      421 aggacgagcg aggtctcgac gatcccgtcg accgcgaccg cggccaggtg cgcggaggcc
      481 gccgcgatcg tgcaggcgtc ggccttcttc gggtcgagcc cgtggtggac cgaatgcagc
      541 gcgttctcga acgcgaagag cgtcagcagc agggcgaggc acagcaccag ggtccggcgc
      601 gggcgccggc gcagcgccac cccggccagc accagggcgc ccgccgcgat ccatggcgtg
      661 aagccgctgg aatcggcggc cgcggcgacg gcggggaccg gtgacagggt caccggcggg
      721 atcgacgcct cggcggtcga agccacgggc tcgatgatcg acgcggacga gtgggccatc
      781 gcgggcccgg tggccgcgag cagggccacc gcggccagca gtacaccggt gagcacgatc
      841 gtccggttgg ctgacattgc aggcagggta ccgaatgaac ccgcgagcgt caatgacggt
      901 tccgctcgag tcgttgacgc ggccggagtc gctgtgggat cataacgtcc cgccatgaaa
      961 gtccatttca cgacactcgg gtgccccaag aaccaggtgg actccgagct gatgctggga
     1021 atgctgaccg aggccggcca cgagatcgcg gcggccgccg agggggccga ctgcctggtg
     1081 gtcaacacct gcgccttcat cgaccgggcc cgcgaggaat cggtcaacac cattctcgag
     1141 ctggctcatc tgaaggagcg cgggcgggcc cgggcgctca tcgtcaccgg ttgcctcacc
     1201 cagcgctacg ggctcgagat cagcaaggag atcccggagg tcaacgcgat cctcggcacc
     1261 tcggagctgc accgcatcgt ggacctggtc aaccaggccg acggtcgcca ggcgtgggtg
     1321 agcgccgcgc cgcccggata cctctacgat gcgcagaccc cgcggctgct caccggccgg
     1381 gtgccctacg cctacgtcaa gatcgccgag ggctgcgaca tggggtgcac cttctgtgcg
     1441 atccctcagt tccgcggcaa gcaccgcagc cgggccctga cggacatcgt cgcggaggtg
     1501 agcgggctcg cccggcgcgg catccaggaa gcgatcctgg tgtcgcagga cacgctcgcc
     1561 tacgggcgcg acctgcccgg caacggggac atcggggacc tgctcctggc gctgtccgac
     1621 acgccgatgc cctggatccg gcccatgtat ctccaccccg cccacgtgac cgagcgcttg
     1681 atcgagaagt ggcggcgcgc gcgggtggtg ccctatctcg acatgccggt gcagcacggc
     1741 gacgacgcga tcctgcgcgc catgcgtcgc ggcgtgaccg cccagcgcat gaaggacatc
     1801 gtggcgcggc tgcgcgacgc gctgcccggc gtcaccctgc gcaccacgct cctggtgggc
     1861 tttccgggcg agaccgaggc cgcgttccag aacctgctcg ccttcgtgga ggacgtggcg
     1921 ttcgaccgcc tcggcgtctt cacctactcg gtggaggagg gcacgcccgc cgccgacatg
     1981 ccggaccagg tcccggccga ggtgatggcg gagcgggcgc agcaggtgca ggacctccag
     2041 gatcgcctgg cgtggccgcg ccagaaggcg ctcttcggca cgcggcagac cgtgctggtc
     2101 gacgggccga gcccggatcc ggcgttcccc ttcgagggcc gcaccgcggg ccaggcgccc
     2161 gagatcgacg gcgtggtgct gctgcgcaac gcccgcctca cgcccggacg cttcgcggac
     2221 gtcggcatcg tcgaggtgga cggctacgag ctcgtcggcg aataagccgc tcggcgtcct
     2281 cgatcgcctg gccttcctgt tggccagcgt cttcggggcc ggttactcgc cggtggcgtc
     2341 gggcacggtc ggcagcttcg tgaccgcggt cgcgctctgg ctcctgccct tcacgccgct
     2401 gcggatcgcg gtcaccctgg tggcggtgat cctgatcggc atctgggcgg ggggccgcgt
     2461 cgagcgcgtg ctcggccgca aggatccggg cgtgatcgtc atcgacgagg tcgcgggcat
     2521 gctgctctcg gtgatcgtgc tgccgcggac ccttccggtg ctggtcaccg cgttcctcct
     2581 cttccgcctc ttcgacatct ggaagccgtt cccagcccgt gagagccagg ccctcaccgg
     2641 cggcatgggg gtgatggtgg acgacctcat cgccgggctc tacgccctgg tgctcgtcat
     2701 gggggcgcgg ttccttttcg gcgtgcccgc gtgaggccat gagccagacc tggatcgtca
     2761 cggtgggcgc ggcccggctc ggcgcggccg acgacccggc cgggctcctg gtcgcgcgcg
     2821 cccttctcgc cgacggggtg ccggtggcct cgcgccagat cgtcgacgag gacgagaccg
     2881 ccgtcgagga ggcgctgcgc tccgcggtcg aggtggcctc tctcgtggtc gtgctcgcgc
     2941 accccggggg gtcgagcggc gaggtggtcc ggcgcgcgct gtcgcgg
//
LOCUS       DATMCV010001005         5095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_395665, whole genome shotgun sequence.
ACCESSION   DATMCV010001005 DATMCV010000000
VERSION     DATMCV010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395665"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VNU03_23060"
     CDS             complement(<1..182)
                     /locus_tag="VNU03_23060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWP77422.1"
                     /translation="MTDFGLAIRNFVGPGEVPDIQGLYAYSERAEALGFESLWAWDHV
                     LLGVEPSFPIVDSITML"
     gene            complement(347..1075)
                     /locus_tag="VNU03_23065"
     CDS             complement(347..1075)
                     /locus_tag="VNU03_23065"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWP77423.1"
                     /translation="MGARELRDNTTVFTGVGAPMMASVLAQRTHAPRLTMMVEGGVIG
                     PMWKPGELPISTNEMRAAYRAQMLPGITDAFLLAQRGFFDVGFIGGAQIDRHGNLNTS
                     AIGGYARPKVRLPGSGGANDIISLCREVLILTVHEKRRFTERVDFVTSPGYLDGGDSR
                     RRHGLLFGGVSRVVTTLGIFGFEADSRRMQLLALHPGATVEQVRENTGFEVLVSPTLT
                     TTKPPTEDELAILRMLDPQRQFIG"
     gene            complement(1105..2067)
                     /locus_tag="VNU03_23070"
     CDS             complement(1105..2067)
                     /locus_tag="VNU03_23070"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWP77424.1"
                     /translation="MTIAAREYDFEAARKRLEAKPKSAGPKLRSLEEAAALVKDGDHV
                     ALGGCLYSRTPLALVWALLRRRPRGLTLSRNLMCYEGEWGMVAGAVDKLVTSWMGIGL
                     PWGLSRIQREFVEAGRVEFEEWSHLGLGLRYRAAAMGVPFLPSLTMLGSGLMGVGGSK
                     TVTCPYTDQTLHAVPALFPDVALIHVQKADRLGNCQIDGYPHMDADIARAASTVLVTA
                     EQIVSEDEIRLQGDRTLIPGFMVDALAHVPYGSYPHECYGAYDAEPEHFTSYVDGILA
                     RGSAAVADYLERYVYAPAAHADYLALFGDGRRADAAARARMLTT"
     gene            complement(2064..3122)
                     /locus_tag="VNU03_23075"
     CDS             complement(2064..3122)
                     /locus_tag="VNU03_23075"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HWP77425.1"
                     /translation="MSAETKPQWDRYTGALPSIAAAVGRTPLVRLNRITRDVKPAIYV
                     KVEWYGATGSLKDRIYLHMFERAEARGDLKPGMQVLECSTGNAGIACAWVSAVKGYPC
                     TIVMPEGMSEERKKIMRAYGAELVFTPGGESDVDLSLERLRQIRDAALGRYWVPGQFD
                     NPDNVEAHILTTGPELWEQSGGRIGAFVDSQGSGGLLTGVGRYLRTRDPQVKLYAVEP
                     AECALLSRRAWGHHGIEGIGDGFVPRNLDVSLLTGVITTTTEESVDIARRLAREEGIF
                     CGISSGCNVAAALKLARRHPELPSVVTIINDTGQRYFTTPLCGEAKHVDIPEREHPLD
                     PYTVEQLDRYQKTWEVLA"
     gene            complement(3127..4143)
                     /locus_tag="VNU03_23080"
     CDS             complement(3127..4143)
                     /locus_tag="VNU03_23080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HWP77426.1"
                     /translation="MHTSVPVIDIAPFDPRGEPARPLERDAVVRAVDAACTDIGFFTI
                     AGHGVPAALVDRMLATSRAFFDLPAGEKQQVARPRPEQSRGYLALGAENLSYSRGDAS
                     TTDLKEFFAIGPIDVPDEAYYRRPEAYPSFAPNLWPERPAALRAVWTEYYRAMEGLAA
                     RLMRIFALALHLPEDFFRDKTDRHISGIRANHYPEQVDPPAPGQIRAGAHTDYGAVTI
                     LLPENVPGLQVLNRAGAWVDVSAPPSTFVCNIGDLMQHWTNDRWISTMHRVVNPPREA
                     AAGNRRLSIPFFHQPNYDALIECLPTCCGPDNPARYEAVTSGQHRLTKFLRGVGAVAP
                     GPRG"
     gene            complement(4159..4917)
                     /locus_tag="VNU03_23085"
     CDS             complement(4159..4917)
                     /locus_tag="VNU03_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77427.1"
                     /translation="MTGRLAGSAVVVTGGAAGLGLVYARRFLHEGACVFIADIVDPAG
                     AVASLDAGDRVGGCRVDVASAASVDAMVKTAHRRLGRIDVLINNAAVFASLKPQPFDA
                     IPEEEWDRVMAVNVRGMWNCVKAVAPVMRAQKAGRIVNVASAIVAKGTPYLLHYVASK
                     GAVIAMTRALARELGDDGIAVNAVAPGLILSDTVRANPAVTSFQSAAVLQSRAFKRDA
                     VPEDVEGAVVFLASPDSAFMTGQTLVVDGGSVFV"
     gene            complement(4914..>5095)
                     /locus_tag="VNU03_23090"
     CDS             complement(4914..>5095)
                     /locus_tag="VNU03_23090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWP77428.1"
                     /translation="RLRSLYLPERVESSVAEHRELFEAVVAGDADRGERLARAHADHV
                     LQDALEFTRRAEQDR"
BASE COUNT          731 a         1736 c         1881 g          747 t
ORIGIN      
        1 agcatggtga tggagtccac gatggggaag gagggctcca cgcccagcag cacgtggtcc
       61 caggcccaga gcgactcgaa gccgagggcc tcggcgcgct cggagtaggc gtagaggccc
      121 tggatgtccg gcacctcgcc ggggcccacg aagttgcgaa tcgcgaggcc gaagtctgtc
      181 aactggtatc cccctccgtt ttagccgtct acttcctgaa gcttttcatc cgttttagcc
      241 gtctacttcc ttaagctctt cagctgcggg ggcccagaaa tggcccccgc actcccccag
      301 tggcggacaa ccgtttcgag cagtggcgga caaccgtttc gacggttcat ccgatgaatt
      361 ggcgctgggg gtctagcatg cgcaggatcg ctagctcgtc ttccgtgggg ggcttggtgg
      421 tggtgagggt ggggctcacg aggacctcga agccggtgtt ctcgcgcacc tgctccacgg
      481 tggcgcccgg gtgcagggcg aggagctgca tgcggcggct gtcggcctcg aagccgaaga
      541 tgccgagcgt ggtgaccacg cgcgagacgc cgccgaagag caggccgtgg cggcggcggg
      601 agtcgccgcc gtccaggtag ccggggctcg tgacgaagtc cacccgctcg gtgaagcggc
      661 gcttctcgtg gacggtgagg atcagcacct cgcgacagag cgagatgatg tcgttggcgc
      721 ccccgctgcc gggcaggcgc accttgggcc gcgcgtagcc gccgatggcg ctggtgttga
      781 ggttgccgtg ccggtcgatc tgggcgccgc cgatgaagcc cacgtcgaag aagccgcgct
      841 gggcgagcag gaaggcgtcg gtgatcccgg gcagcatctg ggcccggtag gcagcgcgca
      901 tctcgttggt ggagatcggt agctcgcccg gcttccacat gggcccgatg accccgccct
      961 cgaccatcat ggtgagccgc ggggcgtggg tccgctgggc cagcaccgag gccatcatgg
     1021 gggcgccgac gccggtgaag acggtggtgt tgtcgcgcag ctcccgcgcg cccatcaccg
     1081 cgagcagctc gttggcggtg acgctcacgt ggtgagcatc cgcgcgcggg cggccgcgtc
     1141 ggcccggcgc ccgtcgccga agagggccag gtagtcggcg tgggccgccg gggcgtagac
     1201 gtagcgctcc aggtagtcgg ccaccgcggc gctgccgcgc gccaggatcc cgtccacgta
     1261 gctcgtgaag tgctcgggct ccgcgtcgta ggcgccgtag cactcgtgcg ggtaggagcc
     1321 gtacggcacg tgcgcgagcg catccaccat gaagccgggg atgagggtcc ggtcaccctg
     1381 cagccggatc tcgtcctcgg agacgatctg ctcggcggtc accagcacgg tggaggccgc
     1441 gcgcgcgatg tcggcgtcca tgtgcggata gccgtcgatc tggcagttgc cgagccggtc
     1501 cgccttctgc acgtggatga gagccacgtc gggaaagagg gccggcaccg cgtggagggt
     1561 ctggtcggtg tacgggcacg tgaccgtctt cgagccgccc acgcccatga gccccgagcc
     1621 cagcatggtg agcgagggga ggaacggcac tcccatcgcg gcggcgcggt agcggagccc
     1681 caggcccagg tggctccact cctcgaactc cacgcggccg gcctccacga actcgcgctg
     1741 gatgcgcgag aggccccacg gcaggccgat gcccatccag gacgtgacga gcttgtccac
     1801 cgcgcccgcc accatgcccc actcgccctc gtagcacatg aggttgcgcg agagcgtgag
     1861 gccccgcggc cggcgacgca gcagcgccca caccagggcc aggggtgtgc gcgagtagag
     1921 gcagccgccc agggccacgt gatcaccgtc cttcacgagc gcggccgcct cctcgagcga
     1981 gcggagcttc gggcccgccg acttgggctt ggcctcgagc cgcttccggg cggcctcgaa
     2041 gtcgtactcg cgcgcggcga tggtcacgcg aggacctccc aggtcttctg gtagcggtcg
     2101 agctgctcga cggtgtaggg gtcgagcgga tgctcgcgct cgggaatgtc gacgtgcttg
     2161 gcctcgccgc agagcggggt ggtgaagtag cgctggccgg tgtcgttgat gatcgtcacc
     2221 accgagggca gctcggggtg gcggcgggcc agcttgagcg cggcggccac gttgcagccg
     2281 ctcgagatgc cgcagaagat gccctcctcg cgggccagcc gccgggcgat gtcgacgctc
     2341 tcctcggtgg tggtggtgat cacgccggtg agcagcgaga cgtccaggtt gcgcggcacg
     2401 aagccgtcgc cgatcccctc gataccgtga tggccccacg cccgtcgcga gagcagcgcg
     2461 cactcggccg gctcgaccgc gtagagcttc acctgggggt cgcgggtccg gagatagcgg
     2521 cccaccccgg tgagcaggcc gcccgagccc tgcgagtcga cgaaggcccc gatgcggcca
     2581 cccgactgct cccacaactc ggggccggtg gtgaggatat gggcctcgac gttgtcgggg
     2641 ttgtcgaact ggccgggcac ccagtagcgg ccgagggccg cgtcgcggat ctggcggagg
     2701 cgctcgagcg agagatccac gtcgctctcg ccgcccgggg tgaagaccag ctcggcccca
     2761 taggcgcgca tgatcttctt gcgctcctcg ctcatccctt ccggcatcac gatggtgcag
     2821 gggtagccct tgaccgcgga cacccacgcg caggcgatcc ccgcgttgcc ggtcgagcac
     2881 tcgagcacct gcatgcccgg cttgagatcg ccgcgcgcct cggcgcgctc gaacatgtgc
     2941 aggtagatgc ggtccttgag gctgccggtg gcgccgtacc actcgacctt cacgtagatg
     3001 gcgggcttca cgtcgcgggt gatgcgattg aggcgcacga gcggcgtgcg gcccaccgcg
     3061 gcggcgatgg acggcagcgc gccggtgtag cggtcccact ggggcttggt ctcagcgctc
     3121 atgatgtcag cctcgcgggc ccggcgcgac cgcgccgacg cctcgcagga acttggtgag
     3181 ccggtgctgg ccggacgtga ccgcctcgta ccgggccggg ttgtccgggc cgcagcaggt
     3241 ggggaggcac tcgatcagcg cgtcgtagtt gggctggtgg aagaagggga tcgagaggcg
     3301 gcggttgccg gcggccgcct cgcggggcgg attcaccacg cggtgcatgg tcgagatcca
     3361 gcggtcgttg gtccagtgct gcatgaggtc gccgatgttg cacacgaagg tggacggcgg
     3421 cgcgctcacg tcgacccagg cgcccgcgcg attcagcacc tgcagcccgg gcacgttctc
     3481 gggcagcagg atggtgaccg ccccgtagtc ggtatgcgct cccgcgcgga tctggcccgg
     3541 cgccggcgga tcgacctgct cgggatagtg gttggcccgg atgccgctga tgtggcggtc
     3601 ggtcttgtcg cggaagaagt cttccggcag gtggagcgcc agggcgaaga tccgcatgag
     3661 acgggccgcg agcccctcca tggcccggta gtactcggtc cacaccgcgc gcagcgccgc
     3721 gggccgctcg ggccagaggt tcggcgcgaa cgacgggtag gcctccggtc gccggtagta
     3781 ggcctcgtcc gggacgtcga tcggcccgat ggcgaagaac tccttgagat cggtggtgga
     3841 ggcgtcaccg cggctgtagg agagattctc ggcgccgagc gcgaggtagc cgcggctctg
     3901 ctcgggccgc ggccgcgcca cctgctgctt ctcgccggcc ggcaggtcga agaacgcgcg
     3961 cgaggtggcg agcatgcggt cgaccagcgc tgcgggaacc ccgtgccccg cgatcgtgaa
     4021 gaagccgatg tcggtgcagg ccgcgtcgac cgctcgcacc accgcgtcgc gctcgagcgg
     4081 ccgcgcgggc tcgccgcgcg gatcgaaggg cgcgatgtcg atgaccggga cgctggtgtg
     4141 cacggcagcg cgccccgcct agacgaacac cgagccgccg tccaccacca gcgtctggcc
     4201 ggtcatgaag gcgctgtcgg gggaggcgag gaagaccacc gcgccctcca catcctccgg
     4261 gaccgcgtcg cgcttgaacg cccgcgactg cagcaccgcg gccgactgga acgaggtgac
     4321 ggccggattg gcccgcacgg tgtcgctcag gatcaggccg ggcgccaccg cgttcaccgc
     4381 gatgccgtcg tcgcccagct cccgggccag cgcgcgcgtc atcgcgatca cggccccctt
     4441 ggaggccacg tagtggagca ggtacggcgt gcccttggcc acgatggcgg aggccacgtt
     4501 gacgatgcgc ccggccttct gggcgcgcat caccggcgcg actgccttga cgcagttcca
     4561 catcccacgc acgttgaccg ccatcacccg gtcccactcc tcttccggga tggcgtcgaa
     4621 gggctgcggc ttgaggctgg cgaacaccgc ggcgttgttg atcaggacgt cgatgcgccc
     4681 gaggcgccgg tgggccgtct tcaccatggc gtccaccgat gcggcggagg ccacgtcgac
     4741 ccgacagccg cccacccggt cgcccgcgtc gaggctcgcg accgctccgg ccgggtccac
     4801 gatgtcggcg atgaagacgc aggcgccctc gtgaaggaag cggcgcgcgt agaccaggcc
     4861 gaggccggcc gcgcctccgg tgaccacgac cgcgctgccc gcgagccgcc cggtcaccga
     4921 tcctgctcgg cccgacgcgt gaactcgagc gcgtcctgca gcacgtggtc ggcgtgggcg
     4981 cgggccaggc gctcgccgcg atcggcgtcg cccgccacca ccgcctcgaa gagctcgcgg
     5041 tgctcggcca cgctcgactc gacccgctcc ggcaggtaga gcgaccggag gcgga
//
LOCUS       DATMCV010001006         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_122884, whole genome shotgun sequence.
ACCESSION   DATMCV010001006 DATMCV010000000
VERSION     DATMCV010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122884"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..697)
                     /gene="greA"
                     /locus_tag="VNU03_23095"
     CDS             complement(197..697)
                     /gene="greA"
                     /locus_tag="VNU03_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWP77429.1"
                     /translation="MTETREETVTWLTQEAYDRLKAELDYLSGEGRIDIAKKIEAARD
                     EGDLRENGGYHAAKDEQGQIEARIITLQHILENARVGAPARTDGVVGPGMTVTIRFVK
                     DEDEVTFLLASREESGSPIDVYSPRSPLGAAIMGKKVGERATYSLPNGRANTVEILEA
                     VPYIGA"
     gene            903..1790
                     /gene="mca"
                     /locus_tag="VNU03_23100"
     CDS             903..1790
                     /gene="mca"
                     /locus_tag="VNU03_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HWP77430.1"
                     /translation="MQSQLRLMAVHAHPDDESSKGAATMARYVGEGAQVLVCTLTGGE
                     RGDILNPAMERPEVKADMARIRREEMARAREILGVQQSFLGFVDSGLPEGDPKPPLPD
                     GCFALTPLAEAVAPLVRAVREFRPHVMLTYDENGGYPHPDHVMTHKVSVAAFEAAGDP
                     DAYPDCGEPWQPLKLYYFVSFHGAKYIAMHEEMLSRGLESPYAKIFEERSKRRGDDWK
                     ELEITTRVPCGDFFAVRDEALKAHATQVDPNGFWFSCPLEVQQAAWPTEDYHLARSVV
                     DTELPEDDLFAGIRETVSL"
     gene            1787..2008
                     /locus_tag="VNU03_23105"
     CDS             1787..2008
                     /locus_tag="VNU03_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014674634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77431.1"
                     /translation="MTLAALAAAAASSSVNDVEPGVLGFLVVAAMGVALVFLLRSMNK
                     QFRKITPEPDAPDREQAGEQAKASAQTPE"
BASE COUNT          319 a          715 c          719 g          353 t
ORIGIN      
        1 gcaggccgca ccagcgcgcc cgggcgcgga tcgccgccgc aggctcatca tcgcggccgt
       61 catcgccgtc ctggtgatcg ccgtcgtcgc cgtcctcgtg ttcgggcatg tgttccgcgt
      121 ctacgggtcc ggaaacggtt agcgagaacg ggcagtggta agtgatacca ctgcccgttc
      181 ggcataaaaa ggggttttac gccccgatgt acgggacggc ctccaggatc tcgacggtgt
      241 tggcgcggcc gttcggcagt gagtaggtgg cccgctcgcc gaccttcttg cccatgatcg
      301 cggcgccgag cggggagcgg ggcgagtaga cgtcgatcgg cgacccgctc tcctcgcgcg
      361 aggcgagcag gaacgtcacc tcgtcctcgt ccttgacgaa ccggatggtc accgtcatgc
      421 ccggcccgac cacgccgtcg gtgcgcgccg gggcgcccac ccgggcgttc tcgaggatgt
      481 gctggagcgt gatgatgcgc gcctcgatct ggccctgctc gtccttggcc gcatgataac
      541 cgccgttctc acgcaggtcg ccctcgtccc gggccgcctc gatcttcttc gcgatatcga
      601 tgcgcccctc ccctgagagg tagtcgagct cggccttgag ccggtcgtac gcctcctggg
      661 tcagccaggt gacggtttct tcacgcgtct cggtcacggg tcactccttg gcactgcgca
      721 ggacggacat gtccgcggga ccctcgtcgg tcctcggggg taatctgctg aggctaacta
      781 gcgtcggtga ctagagcgat ctagtcaaat gcggaatctt acctcgcccg gtggactggg
      841 aatgtcggcc tggtaaacgg gtgtttgcgc caccgagggt tttttcacga gggagaccac
      901 tgttgcaaag ccagcttcgg ctcatggccg tgcacgccca cccggacgac gagtccagca
      961 agggggcggc cacgatggcg cgttacgtcg gcgagggcgc gcaggtcctg gtgtgcacgc
     1021 tgaccggcgg ggagcgcggc gacatcctga acccggccat ggagcggccc gaggtcaagg
     1081 ccgacatggc ccggatccgg cgggaggaga tggcgcgggc ccgggagatc ctcggggtgc
     1141 agcagagctt cctcgggttc gtcgactcgg gcctgcccga gggcgacccc aagccgccgc
     1201 tgccggacgg ctgcttcgcg ctcaccccgc tcgccgaggc ggtcgcgccg ctggtgcgcg
     1261 cggtccggga gttccgcccg cacgtgatgc tcacctacga cgagaacggc ggctacccgc
     1321 acccggacca cgtgatgacc cacaaggtca gcgtggcggc cttcgaggcg gccggcgacc
     1381 cggacgcgta ccccgactgc ggtgagccct ggcagccgct caagctgtac tacttcgtct
     1441 cgttccacgg cgcgaagtac atcgcgatgc acgaggagat gctcagccgc ggcctggagt
     1501 cgccctacgc caagatcttc gaggagcgct cgaagcggcg cggcgacgac tggaaggagc
     1561 tcgagatcac gacccgggtg ccgtgcggtg acttcttcgc ggtccgggac gaggcgctca
     1621 aggcccacgc cacccaggtc gacccgaacg gcttctggtt ctcctgcccg ctggaggtgc
     1681 agcaggccgc gtggccgacg gaggactatc atctggctag gtcagttgtc gacaccgaac
     1741 tgcccgaaga tgacctgttc gccggcatcc gggagactgt gagcctatga ccctcgccgc
     1801 gctcgcggcc gccgccgcgt cctccagcgt caacgacgtg gagcccggcg tcctgggctt
     1861 cctggtcgtg gccgcgatgg gcgtggcgct ggtcttcctg ctccgctcga tgaacaagca
     1921 gttccgcaag atcactcccg agccggatgc ccccgaccgc gagcaggccg gcgagcaggc
     1981 caaggcctcg gctcagactc cggagtaacc gggccagcat tttcgcgtcg ccaggtcata
     2041 accaatcggc cggggccgcc gcggtgctac ggtaatgcgc acctggcggc ttatctcatg
     2101 gcaaac
//
LOCUS       DATMCV010001007         3068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_927161, whole genome shotgun sequence.
ACCESSION   DATMCV010001007 DATMCV010000000
VERSION     DATMCV010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927161"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VNU03_23110"
     CDS             complement(<1..783)
                     /locus_tag="VNU03_23110"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77432.1"
                     /translation="MTMRRRAFLETAAAGALAVPLWRGKARAQAKPVRIGYAISATGP
                     YAVGAGITQAPNYTLWQDQVNARGGLAVKGEGRRPIEFVSIDDRSEIETAVRFYEKLA
                     TDDKVDLMLPPWGSAMNFAIAPVATKHGYPMIGPTAISGKFKELALPYFYTMLVQPDP
                     QMQAVAAKLKDLREQGKIKKVAVAYVNDLFGIELNAATGPAFKAAGLEVVDTKSYPLA
                     VKDLSPVLRGFKAAGADAFVGLTYPPDNILVTGQAKEVDWNPA"
     gene            complement(825..1544)
                     /locus_tag="VNU03_23115"
     CDS             complement(825..1544)
                     /locus_tag="VNU03_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77433.1"
                     /translation="MTRPFLEGTDVARSFGGLRVLHRVSFAVAEGEIVALIGPNGAGK
                     TTLFNLINGLLRPSAGSIRMGGREISALPAYRIARLGLGRTFQTPRPFLDLTAAENVA
                     AAARWSERPGGTPAELLALVELGEQAAVPARRLTGGRRKLLELAMALAMRPRVILLDE
                     LLAGLTPTEGARATAILRRIRDERGVGLFWVEHVMKAIMETAERLIVLHHGEVICEGP
                     PAAVARDPRVLEAYLGEAIAS"
     gene            complement(1549..1980)
                     /locus_tag="VNU03_23120"
     CDS             complement(1549..1980)
                     /locus_tag="VNU03_23120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWP77434.1"
                     /translation="MTSPILFAVIAGLSAAAYTICLKLGSSRISAVLGAVVITGVAFV
                     VNSIALLAMRSAGHELLLTREGLWLLAAAGVAASGVDIFGLLAYERGLRVTSSFLIGG
                     TSTVVVLLVGFLVLQEPLTWGRLAAIAMIVGGTLLYQLQGR"
     gene            complement(2021..2458)
                     /locus_tag="VNU03_23125"
     CDS             complement(2021..2458)
                     /locus_tag="VNU03_23125"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HWP77435.1"
                     /translation="MLLDLVALLAVGLFAGAALYVTLVEHPARRQCGAALAIAEFGPS
                     YRRGAVMQASLAAVGGLAGVVDWTRGHGALPLAAGLLMVGLIAFTLIVILPTNKRLLD
                     PALDPGSAEATALLARWGRLHAVRSGLGTLALLLLGLHLVGVR"
     gene            complement(2462..>3068)
                     /locus_tag="VNU03_23130"
     CDS             complement(2462..>3068)
                     /locus_tag="VNU03_23130"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWP77436.1"
                     /translation="EGGWWRGPERVDWSGPLRAWYGTLRLRDDQVAAIRWLAAQPKGP
                     AKILMISEEWSSDCRRVIPMLGRLVEAAPLELRIFPRDGRKVGREPRADPAGSPNADL
                     MNEFLRERDGRTYQSIPVVAFYTKDLEYLYHYTELPALFPRDRLVPVMQAARPGETRE
                     QAWERFLSDWGAMQQSPIFKVWASGAIDEMLSALYERLVIG"
BASE COUNT          464 a         1091 c         1102 g          411 t
ORIGIN      
        1 cgccgggttc cagtccacct ccttggcctg cccggtgacc aggatgttgt ccggcgggta
       61 ggtgaggccg acgaacgcgt ccgcgcccgc cgccttgaag ccgcggagca ccggcgagag
      121 gtccttgacc gcgagcgggt agctcttggt gtcgaccacc tcgagcccgg ccgccttgaa
      181 ggcggggccg gtggccgcgt tcagctcgat gccgaagagg tcgttgacgt aggccaccgc
      241 gaccttcttg atcttgccct gctcgcgcag gtctttgagt ttcgccgcca ccgcctgcat
      301 ctgcggatcg ggctgcacga gcatggtgta gaagtagggc agggcgagct ccttgaactt
      361 gcccgagatc gcggtggggc cgatcatcgg gtagccgtgc ttggtggcca ccggcgcgat
      421 cgcgaagttc atggccgagc cccacggcgg cagcatcagg tccaccttgt cgtcggtggc
      481 gagcttctcg tagaagcgca ccgcggtctc gatctcgctg cggtcgtcga tcgagacgaa
      541 ctcgatcggc cggcgcccct cgcccttgac cgcgaggccg ccgcgcgcgt tcacctggtc
      601 ctgccagagc gtgtagttgg gcgcctgggt gatgccggcg ccgaccgcgt aggggccggt
      661 ggccgagatc gcgtagccga tgcggaccgg cttggcctgg gcccgcgcct tcccgcgcca
      721 gagcggaacc gcgagcgcgc ccgccgccgc cgtctcgagg aatgcccgtc tgcgcattgt
      781 catgtccgtg gcctccttgc cagtgggatg tgaggtgatg ccggtcagga cgcgatcgcc
      841 tcgcccagat acgcctcgag cacgcgcggg tcgcgcgcca ccgcggccgg cggcccctcg
      901 cagatgacct cgccgtggtg cagcacgatg agccgctccg cggtctccat gatggccttc
      961 atcacgtgct ccacccagaa cagcccgacc ccgcgctcgt cgcggatgcg tcgcaggatc
     1021 gcggtggcgc gcgcgccctc ggtcggggtg agcccggcca gcagctcgtc cagcaggatc
     1081 acgcgtggcc gcatggccag cgccatcgcc agctcgagca acttgcggcg cccgccggtg
     1141 agccggcggg ccggcaccgc cgcctgctcg cccagctcga ccagggccag cagttcggcc
     1201 ggggtgccgc ccggccgctc gctccagcgc gccgccgcgg ccacgttctc ggccgcggtg
     1261 aggtcgagga agggccgcgg ggtctggaag gtgcgcccca ggccgagccg ggcgatccgg
     1321 taggccggca gggcactgat ctcccgcccg cccatccgga tggacccggc ggacggccgg
     1381 agcagcccgt tgatcaggtt gaagagggtg gtcttgccgg ccccgttcgg cccgatgagg
     1441 gcgacgatct cgccctcggc taccgcgaag ctcacccggt ggagcacccg caggcccccg
     1501 aagctccggg cgacgtcggt gccctcgagg aagggccggg tcatcgcgct agcgaccctg
     1561 caactggtag agcaatgtcc ccccgacgat catcgcgatc gcggcgaggc ggccccaggt
     1621 gagcggctcc tggagcacga ggaaacccac cagcagcacg accacggtgg aggtgccgcc
     1681 gatgaggaac gacgaggtca cccgcaggcc gcgctcgtag gcgaggagcc cgaagatgtc
     1741 gacgccggaa gcggcgacgc cggccgccgc gaggagccag agcccctcgc gggtgaggag
     1801 gagctcgtgc ccggccgacc gcatggccag cagcgcgatc gagttgacca cgaaggcgac
     1861 gccggtgatc accacggccc cgagcaccgc gctgatccgc gaggagccga gcttgaggca
     1921 gatcgtgtag gcggcggcgg acaggccggc gatcaccgcg aagaggatgg ggctggtcac
     1981 gcgccgaaga tatcccgaaa gcgcgcccgc gtcatgcgcg tcatcggact ccgaccagat
     2041 ggaggccgag caggagcagc gcgagggtgc cgagtccgct gcgcaccgcg tgcaaccggc
     2101 cccagcgggc caggagcgcg gtggcctcgg cggagcccgg atcgagggcc ggatccagca
     2161 ggcgcttgtt ggtcggcaag atgacgatca gggtgaaggc gatcaggccc accatcagca
     2221 gcccggcggc gagcgggaga gctccgtggc cgcgggtcca gtcgaccacg cccgcgaggc
     2281 cgcccaccgc ggcgagcgac gcctgcatga ccgctccccg gcgatagctc ggcccgaact
     2341 ccgcgatcgc gagcgcggct ccgcactgac gccgggccgg gtgctcgacc agggtgacat
     2401 agagcgcggc cccggcgaag agcccgacgg ccagcagcgc gacgaggtcg aggagcatgg
     2461 ctcagccgat caccagccgc tcgtacagcg ccgagagcat ctcgtcgatg gccccggagg
     2521 cccacacctt gaagatgggc gattgctgca tcgcgcccca atcgctcagg aagcgctccc
     2581 acgcctgctc ccgggtctca cccggccggg ccgcttgcat gacggggacc aggcggtccc
     2641 ttggaaagag cgcggggagc tccgtgtagt gatacaggta ctcgaggtcc ttcgtgtaga
     2701 aggccacgac cgggatcgac tgataggtcc ggccgtctcg ctcccgcagg aactcgttca
     2761 tgagatcggc gttcggcgac ccggcgggat cggctctcgg ctcccgcccc accttccggc
     2821 cgtcgcgcgg gaagatgcgg agctcgagcg gagcggcctc caccagacgc ccgagcatgg
     2881 gaatgacccg gcggcagtcc gacgaccatt cctccgagat catgaggatc ttggccggcc
     2941 ccttcggctg tgcggcgagc cagcggatcg ctgcgacctg atcgtcgcgg agccggaggg
     3001 ttccgtacca cgcccgcagc gggccgctcc agtcgacccg ctccgggccc cgccaccagc
     3061 cgccctcg
//
LOCUS       DATMCV010001008         2041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_521339, whole genome shotgun sequence.
ACCESSION   DATMCV010001008 DATMCV010000000
VERSION     DATMCV010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521339"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VNU03_23135"
     CDS             complement(<1..670)
                     /locus_tag="VNU03_23135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77437.1"
                     /translation="MHAALRSTLVALCLSLHSPGVGAESERSTFHLSTEFHGPLGYTN
                     PDVARNLRNGMANMVAFWPPADVGMRNFVVTHDVRLSFRLPGASHVNNESAGSIESQR
                     LAAARICAAQPAKSHLWNLMIEWDQSGGAWVPNGRPRYGALTRPQAHARFVDHYLKQS
                     PPLATYLQQPPTARPCRLAAVTDHSPNAFDAYELGVDVGLLERGIDELGDIATGIAFM
                     RGAGR"
     gene            complement(679..1164)
                     /locus_tag="VNU03_23140"
     CDS             complement(679..1164)
                     /locus_tag="VNU03_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77438.1"
                     /translation="MRPSGPRGNPAAALLGGLACGALWFLFATMILDVVRPGATIVPT
                     LAVAAVMGLAAAAFCWRPGTSREVWGRVALTVGLHALAMPVAAAISFGATGIWPPPET
                     ADLGLSVDVFGVRLVGTPTTVRLGVCGFVGGLLLVAIGDRALGRRGLPDGVRRPGRLS
                     R"
     gene            1323..>2041
                     /locus_tag="VNU03_23145"
     CDS             1323..>2041
                     /locus_tag="VNU03_23145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77439.1"
                     /translation="MSSVAPPAAALAAPQAFASVLRERPYRRLWLSGLCVNGARWMDL
                     ILLGWLAFQLTDSPFMVGLAAFARSAPLMVLGPFTGIVADRMHRGRVLVFTQSLGLVT
                     ALALAAVFASGRGGFWPLVGLETLFGALWALDFPARRTALYALVGSRRVATAISLETV
                     SMQVAKMVGPVLAGVGLAHLGPAPCYLGVAALYAIGLLVFVGLPGRIGGPSGRDVTSV
                     VASLGAGFREAWREPTVRAVLL"
BASE COUNT          257 a          730 c          764 g          290 t
ORIGIN      
        1 gacggccggc gccgcgcatg aaggcgatgc cggtcgcgat atccccgagc tcgtcgatgc
       61 cgcgctcgag gaggccgacg tcgacgccca gctcgtaggc gtcgaacgcg ttcggagaat
      121 ggtcggtgac cgcggcgagg cggcagggcc gcgcggtcgg gggctgttgc aggtaggtgg
      181 cgagcggcgg agactgcttg aggtagtggt cgacgaagcg cgcgtgcgcc tgcgggcgcg
      241 tcagcgcccc gtatcgcggc cgcccgttcg ggacccaggc accaccgctc tggtcccact
      301 cgatcatcag gttccagagg tggctcttgg ccggctgcgc cgcgcagatt cgagcggccg
      361 cgaggcgctg gctttcgatc gagccggccg actcgttgtt gacgtgggag gcgcccggca
      421 gacgaaagct gagccgcaca tcgtgcgtca ccacgaagtt ccgcatgccg acgtccgcgg
      481 gcggccagaa ggcgaccatg ttggccatgc cgttgcgtag gttccgcgcg acgtcgggat
      541 tggtgtagcc gagagggccg tggaactcgg tcgacaggtg aaacgtggag cgctcggact
      601 ccgcgcccac gccgggggag tggagcgaga gacacagtgc cacgagcgtc gagcgcaggg
      661 cggcatgcac cgggcgactc agcgggagag ccgcccgggg cgtcgcaccc cgtccggaag
      721 gccccggcgc cccagggcgc gatcgccgat cgcgacgagg aggagacccc cgacgaagcc
      781 gcagaccccg agccgcacgg tcgtcggcgt gccgaccaac cgcaccccga agacgtcgac
      841 gctgagcccg aggtcggcgg tctcgggagg cggccagatc ccggtggccc cgaacgagat
      901 ggcggccgcg accggcatcg cgagggcatg gaggcccacg gtgagggcca cccggcccca
      961 gacctcccgg gacgtgccgg ggcgccagca gaacgcggcc gccgcgaggc ccatcacggc
     1021 cgcgacggcg agcgtgggca cgatcgtcgc tcccgggcgg acgacgtcca ggatcatggt
     1081 cgcgaacagg aaccagaggg cgccgcacgc caggcccccg agcagcgcgg cggcgggatt
     1141 ccctctcggg ccggatggtc gcatcgcccc gctacttcac gaaggccgcg acacggggac
     1201 gagaggggca gaggtcatgc ggttggctcg ggattgcgaa atgatcgtcc cgcatcgccg
     1261 ggcgtgtcaa tagtcgatcg cgccggttcc cctcgagccg ccttccgtgg tatccctacg
     1321 cggtgagcag cgtcgctccg ccggcggccg ccctggccgc cccccaggcg ttcgcgtccg
     1381 tcctgcgcga gcgcccctat cgccggctct ggctctcggg cctgtgcgtc aacggcgcgc
     1441 gctggatgga tctgatcctc ctcggctggc tcgcgttcca gctcacggac tcgccgttca
     1501 tggtcggcct ggccgccttc gcccgctccg cgccgctgat ggtcctgggt cccttcaccg
     1561 gcatcgtggc cgatcgcatg caccgcgggc gcgtgctggt gttcacccag tcgctcggcc
     1621 tcgtgaccgc gctcgcgctg gccgcggtgt tcgcgtccgg gcgcggcggc ttctggccgc
     1681 tggtcggtct cgagacgctc ttcggcgcgc tctgggcgct cgactttccg gcgcggcgca
     1741 ccgcgctcta cgccctggtc ggctcgcgcc gcgtggccac cgcgatctcg ctcgagacgg
     1801 tgtcgatgca ggtggccaag atggtcggcc cggtcctcgc cggggtgggc ctcgcgcacc
     1861 tcggccccgc gccctgctac ctgggggtgg ccgctctcta cgccatcggc ctgctcgtct
     1921 tcgtgggcct gcccgggcgc atcggcgggc cgagcgggcg cgacgtcacc tcggtggtgg
     1981 ccagcctcgg cgccggcttc cgcgaggcgt ggcgcgagcc caccgtgcgc gcggtgctgc
     2041 t
//
LOCUS       DATMCV010001009         2783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_189220, whole genome shotgun sequence.
ACCESSION   DATMCV010001009 DATMCV010000000
VERSION     DATMCV010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189220"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VNU03_23150"
     CDS             complement(<1..199)
                     /locus_tag="VNU03_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77440.1"
                     /translation="MRGAIAGALALALACYAMPAAAQQPEAKPSPAAARKDTAKMGLR
                     SVTGSVKTATEKGLVVMGHEQG"
     gene            complement(204..728)
                     /locus_tag="VNU03_23155"
     CDS             complement(204..728)
                     /locus_tag="VNU03_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77441.1"
                     /translation="MADKDYLATRLFTDRDSAEREYQATLARGYDPKDINVVMTEEGR
                     KRHFGQGATTELGNKALEGAGVGGGIGAVTGGALAAIAVAATVAVPGLGLLVAGPIAA
                     ALAGAGAGAVVGGGIGALVGAGIPEERVKEYESGLKNGGILMGVKPRTPEDRAYFDEE
                     WGGSQRPMTTGRSV"
     gene            complement(999..1547)
                     /locus_tag="VNU03_23160"
     CDS             complement(999..1547)
                     /locus_tag="VNU03_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77442.1"
                     /translation="MICALVVLLAVGLTAPLGYAQTGAATEDSLRAWGRVAEVLRHPR
                     CMNCHPSGDVPRQTDDRHLHRMLVMRGVDDRGTAAMRCTTCHQSVNTADGRVPGAPHW
                     HLAPRPMGWEGLSDGELCRSVKDPARNGGRSVPALVQHMTADALVQWAWNPGARTAPP
                     VSQFDFHEAVKRWAVTGAACPS"
     gene            complement(1559..>2783)
                     /locus_tag="VNU03_23165"
     CDS             complement(1559..>2783)
                     /locus_tag="VNU03_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008074358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP77443.1"
                     /translation="LRRGDADAALVGAARRVTAQYDMPYLAHAPMEPLNCLVSLGPER
                     CGIWTGTQFQTVDQANAARAAGLKPEQVAIHTMFLGGGFGRRAVPGSDFIVEAVHVAK
                     AAGAPVKTIWTREDDIRGGYYRPMWHSSLSAGLDAAGKPVAWKHTIVGQSIVENTPFA
                     GEIKNGVDGTSVEGAANLVYEVPNAAVDLHSPRSPVPVLWWRSVGHTHTGFVVESFID
                     ELAHAAGQDSYAYRRELLAGKTRHLGVLELAAQKAGWGTPLPAGRARGIAVHASFGSF
                     CAQVGEVSVQDRAITVHRVVCAVDCGRAINPETIRAQMEGGIVFGLSAALYGQVTMKR
                     GRVQQSNFHDYPVLRLNSMPVVEVHIVPSTEPPSGIGEPGTPLMAAVLGNALFTLTGQ
                     RVRSLPLVRHGFESA"
BASE COUNT          399 a         1031 c          927 g          426 t
ORIGIN      
        1 ggccttgctc gtgtcccatg acgacgagcc ccttctcggt agccgtcttc acggagccgg
       61 tgacacttcg caggcccatc ttcgcggtat ccttgcgcgc cgcggccggg ctcggcttgg
      121 cctccggctg ctgggccgcg gccggcatcg catagcacgc gagtgctaac gccagcgccc
      181 cggcgatcgc tccacgcatg gcgctagacg ctccgacccg ttgtcatcgg cctctgactg
      241 ccgccccact cctcgtcgaa ataggcacgg tcttcgggcg tacgcggctt cacccccatg
      301 aggatgccgc cattcttcag accgctctcg tactccttga cgcgctcttc gggaatgccc
      361 gcaccgacca gcgccccgat tccgccgccg acgacggcgc ccgccccagc tcccgccagc
      421 gcggcggcaa tcggccccgc gacgagcaga ccgaggcccg gcaccgcgac cgtcgccgcc
      481 actgcgatag ccgcgagagc accacccgtt accgcaccga tgccgccgcc aactccggcc
      541 ccctcgaggg ccttgttgcc gagttcggtc gtcgcgccct gaccgaagtg ccgcttgcga
      601 ccctcttcgg tcatcaccac gttgatgtcc ttcggatcgt acccgcgcgc gagggtcgcc
      661 tggtactcgc gctctgcact gtcccggtcc gtgaatagtc gagttgcgag atagtccttg
      721 tccgccatgg tctgctgctc cttcgggaat cgcccgatga ttgagtggaa tcactgaagc
      781 ggtgccgaag acgcctcgtc ggcgccgcac cgctcacact tggggactac gcatacaaca
      841 tgcccgttgc ggagagcacg gacatcgcgt tcggaagggc gaacgcgtca ttcatctgga
      901 gcgcgggcgc ttctggatgc ggggggcggt ccttacgacc cggtaaggac ggggagcgct
      961 agagcgtctt cagaatcgag cggctcttca ggctccggtc aggatgggca ggccgccccg
     1021 gtcaccgccc agcgcttgac cgcctcgtgg aagtcgaact gcgagaccgg cggcgcagta
     1081 cgcgcgcccg gattccacgc ccactgcacg agcgcgtcgg cggtcatgtg ctggacgagc
     1141 gcgggcacgg atcggccgcc gttgcgcgcc gggtccttga ccgatcggca cagctcgccg
     1201 tcgctgagcc cttcccagcc catcgggcgt ggcgccaggt gccagtgagg cgccccgggc
     1261 acgcggccat ccgcagtgtt gaccgactga tgacaggtgg tgcagcgcat cgcggcggtg
     1321 ccccggtcgt cgaccccccg catcaccagc atgcggtgga gatgtcgatc gtcggtctgg
     1381 cgtggcacgt cgccgctcgg gtggcagttc atgcagcgcg ggtgccgcag cacctccgcc
     1441 acgcgccccc acgcccggag cgagtcctcg gtcgcggcac cggtctgcgc gtatcccagc
     1501 ggggcggtca gtccgaccgc taggagcacc acgagcgcgc agatcatggg gcgccccatc
     1561 atgcggactc gaaaccgtgg cggacgagcg gcagcgagcg gacgcgctgg cccgtgaggg
     1621 tgaagagcgc gttgcccagg accgcggcca tcagcggcgt ccccggctcc ccgatgcccg
     1681 agggcggctc ggtgctgggc acgatgtgca cctccaccac cggcatcgag ttgagccgca
     1741 gtaccgggta gtcgtggaag ttgctctgct gcacccggcc ccgcttcatg gtgacctgac
     1801 cgtagagcgc cgcggacaga ccgaacacga tgccgccctc catctgggcg cggatggtct
     1861 cggggttgat ggcccgcccg cagtccaccg cgcagaccac gcggtgcacc gtgatcgcgc
     1921 gatcctggac ggagacctcg cccacctgcg cgcagaagct gccgaacgag gcgtgcaccg
     1981 cgatgccccg cgcgcggcca gcgggcagcg gcgtgcccca tcctgccttc tgggcggcca
     2041 gctccagcac gccgagatgc cgcgtctttc ccgcgagcag ctcgcgccgg taggcgtacg
     2101 agtcctggcc cgccgcgtgc gccagctcgt cgatgaagct ctccaccacg aagccggtgt
     2161 gggtgtggcc gacggaccgc caccagagga cgggcaccgg cgaccgcggg ctgtgcaggt
     2221 cgacggcagc gttggggacc tcgtagacca ggttcgccgc gccttccacc gacgtcccgt
     2281 cgacgccgtt cttgatctcc cccgcgaacg gtgtgttctc gacgatcgac tgaccgacga
     2341 tcgtgtgctt ccaggccacc ggcttgcccg cggcgtcgag ccccgcgctg agcgaggagt
     2401 gccacatcgg ccggtagtag ccgccgcgga tgtcgtcctc gcgtgtccag atcgtcttga
     2461 ccggcgcccc ggcggccttc gccacgtgaa ccgcctccac gatgaaatcg gacccgggca
     2521 cggcacggcg gccgaagccc ccacccagga acatcgtgtg gatcgccacc tgctcgggct
     2581 tgagcccggc cgcccgcgcc gcattggcct ggtcgaccgt ctgaaactgc gtgccggtcc
     2641 agatcccgca gcgctcgggc ccgagcgaga ccaggcagtt gagcggctcc atcggcgcgt
     2701 gcgccaggta cggcatgtcg tactgcgcgg tcacccgccg cgctgcgccg acgagcgccg
     2761 cgtcggcgtc cccgcgacgg agc
//
LOCUS       DATMCV010001010         4264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_460469, whole genome shotgun sequence.
ACCESSION   DATMCV010001010 DATMCV010000000
VERSION     DATMCV010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460469"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VNU03_23170"
     CDS             complement(<1..1135)
                     /locus_tag="VNU03_23170"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWP77444.1"
                     /translation="MTRTGAHLVVEALEAAGVRHLFSLSGNHILSLYDATLGSRLSIL
                     HTRHEAAAVHMADAWGRLTDEPAVALVTAGPGHCNAVSALYTALMAESPLVLLSGHAP
                     LGQLGRGGFQEVDQAAVAAPVAKASWMAKDASRLGEDITAALELARSGRPGPVHVSLP
                     GDLLEAKVSDGAPARAPRDETPASAPAESVERVLALLAAAQRPLVLLGPAMARGARAA
                     AVERLGEAIGVPALPMESPRGFNDPWLHLAAPLLKEVDLLLLLGKKPDFVVRFAQAAN
                     HAPGCRVVQVDADAASLRGGAVQGIVADPAVMADQLAAAASGRAWSHRAWGEEVRRAR
                     ALVPAGWAAARRSEQAPIHPLRVCAALQPHVDRGAILVVDGGEF"
     gene            complement(1152..2213)
                     /locus_tag="VNU03_23175"
     CDS             complement(1152..2213)
                     /locus_tag="VNU03_23175"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWP77445.1"
                     /translation="MTAITDLETPAVTVHLDVMEDNIRRVQAHLDRHRIANRPHIKTH
                     KIPAIGKMQMAAGAVGITCQKLGEVEVFADARVADDVLLTYNILGAAKTERLMALARR
                     VPRLAVVLDNDVVAGELSEAARRAQHDLPFLIECDTGFRRTGVQTPDEALALARQATA
                     RPGLRFKGLMTFPNRDPDTREFFESALPLFSRAGLPVEVVSGGGTPALATVDRFPMLT
                     EHRAGTCVYNDAMVVSSGTATWDQCAMRVRATVVSRPTDDRGVLDAGSKVLTSDLYHM
                     KGYGHLMEYPEAQVTHLSEEHAVVDFAGCAERPKVGEVVSVVPNHCCVVTNMVDEVYG
                     VRRGAVEVVWPVAARGRVR"
     gene            2315..3124
                     /locus_tag="VNU03_23180"
     CDS             2315..3124
                     /locus_tag="VNU03_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77446.1"
                     /translation="MRPKPPGTLIHVAVLGVLGVLIAAPVEAQENFEIQVYGSETVAP
                     GTTMVELHSNVAAVGTTRTENDVLRTQGAFHETLEITQGWTSWLETGFYLFSSIQPDT
                     TWEWVGSHIRPRVRAPESWELPVGLSLSAEIGYQRRAFSPDTWTVELRPIIDKQWSRW
                     YVAINPTFERAIEGESVGRGFEFSPAAKVSYGVTPKVAIGLEYYGALGPVTHLDRGRD
                     QHHQIFPVIDLDLGPRWEFNAGIGFGLTPSTDQLIIKMILGYRFEWAGGHE"
     gene            complement(3145..3954)
                     /locus_tag="VNU03_23185"
     CDS             complement(3145..3954)
                     /locus_tag="VNU03_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWP77447.1"
                     /translation="MIVVTRRRFVAGAVAALGALAAPRPGAAQSASAGPPDPQLLEDL
                     VAANRILVDQGVLDGYGHVSARHDRDAGRFLMSRSLAPELVTAADVLEYGLDSEPVAA
                     GGRTSYLERFIHGEIYRARPDVRAIVHSHSPSVIPFGVTGAPLRPLYHMSAFLAGGVP
                     VWDIHAAAGDSDMLVRTPALGRSLAQALGARPVVLMRGHGAVVVGASLPQVVFRSVYT
                     EVNARLQAQAIALGGGNVTYLSPEEAARAESAVAGTLGRPWELWKRKATGR"
     gene            complement(3964..>4264)
                     /locus_tag="VNU03_23190"
     CDS             complement(3964..>4264)
                     /locus_tag="VNU03_23190"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HWP77448.1"
                     /translation="CEREEKAVREDLPLARTTKARTDVQTIATAVNTYRVTCGALPES
                     LEALVTQTTVGGAPCGPVLGSIPAPPTGWSAYVYARQGDSFTVTSSGNGQTVAAP"
BASE COUNT          589 a         1578 c         1530 g          567 t
ORIGIN      
        1 cgaactcgcc accgtcgacg accaggatcg cgccgcggtc gacgtggggc tggagggccg
       61 cgcacacccg cagcggatgg atgggcgcct gctcggagcg ccgggccgct gcccacccgg
      121 cgggcacgag ggcgcgcgcc cgccgcacct cttcgcccca cgcgcgatgc gaccacgccc
      181 gcccgctcgc ggcggcggcg agctgatcgg ccatcaccgc gggatccgcc acgatccctt
      241 ggacggcccc gccacgaagc gacgcggcat cggcatccac ctgcaccacg cggcagccgg
      301 gggcgtggtt ggccgcctgg gcgaagcgga cgacgaagtc cggcttcttg cccagcagca
      361 ggagcaggtc gacctccttc aggagcggcg cggccaggtg cagccacggg tcgttgaaac
      421 cgcgcgggct ctccatcggg agcgccggca ccccgatcgc ctcgcccagg cgctcgaccg
      481 cggcggcccg cgccccgcgc gccatggcgg ggccgaggag cacgagcggc cgctgcgccg
      541 ccgcgagcag cgcgagcacc cgctccacgc tctcggccgg cgcggacgcg ggcgtctcgt
      601 cccgcggcgc gcgcgcgggc gcgccgtcgc tcaccttggc ctcgagcaga tcgccgggca
      661 ggctcacgtg gacggggccg ggccgcccgc tccgcgccag ctcgagcgcg gcggtgatgt
      721 cctcgccgag ccgactcgcg tccttggcca tccacgaggc tttggccacc ggcgcggcca
      781 ccgcggcctg gtccacctcc tggaacccgc cgcgcccgag ctggcccagc ggggcgtggc
      841 cgctcagcag caccagcggc gactcggcca tcagcgcggt gtagagcgcg gagaccgcgt
      901 tgcagtggcc gggcccggcg gtgacgaggg ccaccgcggg ctcgtcggtg agacggcccc
      961 acgcgtccgc catgtgcacg gccgccgcct cgtgacgcgt gtgcaggatg gagagccggc
     1021 tgccgagggt ggcgtcgtag agcgagagga tgtggttgcc ggagagcgag aagaggtggc
     1081 ggacgccggc cgcctcgagc gcctccacca cgaggtgcgc gccggtacgc gtcatggccg
     1141 ccggcggggc ctcagcgcac ccgcccgcgc gccgcgaccg gccacaccac ctcgaccgcg
     1201 ccccgccgca ccccgtagac ctcgtccacc atgttggtga ccacgcagca gtgattcggc
     1261 accacgctca ccacctcgcc caccttgggc cgctccgcgc atcccgcgaa gtccaccacc
     1321 gcgtgctcct cggagagatg cgtcacctgc gcctccgggt actccatcag gtgcccgtag
     1381 cccttcatgt ggtagaggtc cgaggtgagc accttgctcc ccgcatcgag caccccgcgg
     1441 tcgtcggtgg gccggctgac cacggtcgcg cgcactcgca tcgcgcactg gtcccacgtc
     1501 gcggtcccgc tcgagaccac catcgcgtcg ttgtagacgc aggtgccggc gcgatgctcg
     1561 gtgagcatcg ggaagcggtc gacggtcgcg agcgcgggag tgccgccgcc cgagaccacc
     1621 tcgaccggca ggcccgcgcg cgagaacagg ggcagcgcgc tctcgaagaa ctcgcgggtg
     1681 tcgggatcgc ggttcgggaa cgtcatcaag cccttgaagc ggaggccggg gcgcgcggtg
     1741 gcctgccggg cgagggcgag cgcctcgtcc ggcgtctgca ccccggtgcg gcggaagccg
     1801 gtgtcgcact cgatcaggaa cggcaggtcg tgctgggcgc ggcgcgccgc ctccgacaac
     1861 tcgccggcca ccacgtcgtt gtccagcacc accgccaggc gcggcacccg gcgggccagc
     1921 gccatcagcc gctcggtctt ggccgcgccg aggatgttgt aggtcagcag cacgtcgtcg
     1981 gccacgcgcg cgtccgcgaa gacctccacc tccccgagct tctggcaggt gatgccgacc
     2041 gcgcccgccg ccatctgcat cttgccgatg gccgggatct tgtgggtctt gatgtgcggg
     2101 cggttggcga tccggtgtcg gtcgaggtgg gcctgcacgc gtcggatgtt gtcctccatc
     2161 acgtccaggt gcaccgtgac ggccggcgtc tcgagatcgg tgatcgcggt catggaagcc
     2221 ctcctcgcgg ccggacgcgg cgcttcatac ccggctccgc tctcttatac cagaagtccc
     2281 ttggcctcga gcgacccgcg gcatagactc gggcatgcgt ccgaaaccac ccggaacgct
     2341 gatccacgtc gccgtcctgg gagttctcgg cgtgctcatc gccgcgccgg ttgaagccca
     2401 ggagaacttc gagatccagg tctacgggtc ggagacggtc gcacccggca ccaccatggt
     2461 ggagctgcac agcaacgtgg ctgcggtcgg cacgacccgc accgagaacg acgtgctccg
     2521 gacgcagggg gccttccacg agaccctgga aatcacccag ggctggacgt catggctgga
     2581 gaccggcttc tacctgttca gctcgatcca gcccgatacc acgtgggagt gggtgggcag
     2641 tcacatccgc ccgcgggttc gggcgccgga gagctgggag ctaccggtcg ggctgagtct
     2701 ctccgccgag atcgggtatc agcgacgggc tttctcgccg gacacgtgga ctgtcgagct
     2761 gcgcccgatc atcgacaagc agtggtcgcg ctggtatgtc gcgatcaacc cgaccttcga
     2821 gcgggcgatc gagggcgaga gcgtcgggcg gggcttcgag ttctcccccg ccgccaaggt
     2881 cagttatggc gtcacgccca aggtcgcgat tggcctcgag tactacggcg cgctcggacc
     2941 ggtcacccac ctcgaccgcg gccgcgacca gcaccaccag atcttcccgg tgatcgatct
     3001 cgacctggga ccccgctggg agttcaacgc gggaatcggc ttcgggctca cgccgagcac
     3061 cgaccagctg atcatcaaga tgatcctggg ctatcgcttc gagtgggccg gcgggcacga
     3121 gtaacccggg ccttttcccg taggctagcg gccggtcgcc ttccgcttcc agagctccca
     3181 cggccgcccg agcgtgcccg cgaccgcgga ctcggcgcgc gccgcctcct ccgggctcag
     3241 gtacgtcacg ttgccgccgc cgagcgcgat cgcctgggcc tgcagccggg cgttgacctc
     3301 ggtgtacacg ctcctgaaga ccacctgcgg cagcgacgcg ccgaccacca ccgcgccgtg
     3361 cccgcgcatc agcaccaccg ggcgggcgcc gagggcctgg gccagggatc ggccgagggc
     3421 gggggtgcgg accagcatgt cggagtcgcc cgccgcggcg tggatgtccc agaccggcac
     3481 cccgccggcg aggaacgcgc tcatgtggta gagcgggcgc aggggcgcgc cggtcacccc
     3541 gaacgggatc accgagggcg agtggctgtg gacgatcgcg cgcacgtcgg ggcgcgcgcg
     3601 gtagatctcg ccgtggatga accgctcgag gtagctcgtc cgtccgcccg ccgcgaccgg
     3661 ctcgctgtcg aggccgtact cgagcacgtc ggccgcggtc accagctcgg gcgcgagcga
     3721 gcgcgacatc aggaagcggc ccgcgtcccg gtcgtgccgc gcgctcacgt ggccgtagcc
     3781 gtccagcaca ccctggtcga ctaggatgcg gttggctgcg accaggtcct cgagcagctg
     3841 cgggtccggc gggcccgcgg aggccgactg cgcggcgccc ggccgcggcg cggcgagcgc
     3901 accgagcgcc gccaccgcac cggccacgaa gcgacgacgg gtcaccacga tcatgggggt
     3961 ctcctagggc gcggcgacgg tctgcccgtt gccgctcgac gtgacggtga acgagtcgcc
     4021 ctgccgcgcg tagacgtagg cggaccagcc ggtgggcggc gcggggatgg agccgagcac
     4081 ggggccgcac ggcgcgcccc ccaccgtggt ctgcgtcacg agggcctcga gcgactcggg
     4141 cagcgccccg caggtcacgc gataggtgtt caccgcggtc gcgatggtct ggacgtcggt
     4201 gcgggccttg gtggtgcgcg ccagcggcag atcctcgcgc accgccttct cctcccgctc
     4261 gcac
//
LOCUS       DATMCV010001011         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_993274, whole genome shotgun sequence.
ACCESSION   DATMCV010001011 DATMCV010000000
VERSION     DATMCV010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993274"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VNU03_23195"
     CDS             <1..927
                     /locus_tag="VNU03_23195"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HWP77449.1"
                     /translation="GVRFDDPLEAARGALAAFGAVDLAVAVTHLPLAQDRALVAALPI
                     DAVLGGHDHDPMLVEEGRAVIVKAGSDAVNVGQVEYELRCGAVLSRRQRLIPVDERLA
                     EAPDVADLVHRQAALLARELDTTVGRTTVPLDAHETLVRREETPLGRFFAELMRERVG
                     AQVGLLNGGAIRGNRVIPPGPITKRDIRGLLPFTNTVVLLELTGEALRAALERSVDEL
                     PRPTGHFLQTAGLAFPIDLAQPAGRRVGPITVAGRPLDPAGRYRVATLDYLARGQDGY
                     PMLAGGRVLLGPEDGPGLIETILDGLVAGRSP"
     gene            974..1048
                     /locus_tag="VNU03_23200"
     tRNA            974..1048
                     /locus_tag="VNU03_23200"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1006..1008,aa:Val,seq:gac)
     gene            1184..>2817
                     /gene="thrS"
                     /locus_tag="VNU03_23205"
     CDS             1184..>2817
                     /gene="thrS"
                     /locus_tag="VNU03_23205"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWP77450.1"
                     /translation="MADAEEARHLNLAGEFDCDPEPLPTLRHSTSHVMAQAVKRLFPQ
                     VKVAIGPAIEDGFYYDFLKAEPFTPEDLARIEDTMREIVKSDYPFTREEMPRDRAIAF
                     FEERGEPFKVEILRGLSVPTVSIYRQGDFLDLCRGPHVASTGQLKAFKLLSASGAYWR
                     GDEKNPMLQRIYGTAWLTQEELDKHLWRLEEAKKRDHRKLGRELDLFDFYDIAPGAPF
                     WLPGGMVLVRELEKYARESLDARGYQEIATPLLVNKKLWEQSGHWDLYQENMFKFDVE
                     GDVFSLKPMNCPESAYVYKRALRSYRDLPIRYSEMGRCHRNERSGALSGLVRVRQFTQ
                     DDAHLYVRPEQVQAEITDLLDLVREWYGTFTLQPSYRVATRPPDKLGTEQQWDQAEDA
                     LHQALRANALVYELDKGGGAFYGPKIDIDVEDALGRQWQLATIQVDLTMLPERMQCEY
                     IDTDGQAKRPVVIHRAIFGSYERFVAILTEHFAGVFPTWLAPVQARVLPISEKHGEYG
                     RKVFAALRAARIRAELDDRNEKLGYRIRDAQIRKVP"
BASE COUNT          428 a          988 c          982 g          419 t
ORIGIN      
        1 ggggtgcgct tcgacgatcc gctggaggcg gcgcgcggcg cgctcgccgc ctttggggcg
       61 gtggacctgg ccgtggcggt gacccacctc ccgctcgcgc aggaccgcgc gctggtcgcg
      121 gcgctcccga tcgacgcggt cctgggcggg cacgatcacg atccgatgct cgtcgaggaa
      181 ggccgcgcgg tcatcgtcaa ggcgggctcg gacgcggtca acgtcggcca ggtcgagtac
      241 gagttgcgct gcggcgcggt gctcagccgg cgccagcgcc tcatcccggt cgacgagcgg
      301 ctcgccgagg ccccggacgt ggccgacctc gtgcaccgcc aggccgcgct gctcgcccgg
      361 gagctcgaca cgacggtggg ccgcaccacg gtccccctcg acgcgcacga gaccctcgtc
      421 cgccgggagg agacgccgct cgggcgattc ttcgcggagc tgatgcggga gcgggtcggg
      481 gcccaggtgg ggctgctcaa cggcggcgcc atccggggca accgggtgat tccgcccggg
      541 ccgatcacca agcgcgacat ccgggggctg ctgcccttca ccaacaccgt ggtcctgctg
      601 gagctgacgg gtgaggccct ccgggccgcg ctcgagcgct cggtggacga gctgccgcgg
      661 cccaccggcc atttcctcca gaccgccggg ctggcgttcc cgatcgacct ggcccagccc
      721 gccggccgcc gggtcggccc gatcacggtg gccggccgcc cactcgatcc ggccggtcgg
      781 taccgggtcg ccaccctcga ctacctggcc cgcggccagg atggctaccc gatgctggcc
      841 ggcggccggg tgctgctggg cccggaggac ggccccggcc tcatcgagac gatcctggac
      901 gggctcgtcg cgggccgctc cccttgacgg ggattggggg ctccggtagt gtattcaatg
      961 ctttcgggaa cctaggctcg tagctcagtg ggagagcgct accttgacac ggtagaggtc
     1021 ggcggttcga aaccgcccga gcctaccaat tcctgatgtg gagtgagtcg gctacctggg
     1081 ggtgcgcatc gtttgccggt caagcgtgag ttgagcgagg gagttgcgag catcaccggg
     1141 cgcagccctg tgatgctttg ttgttttctg agacagggag aacatggcgg acgctgaaga
     1201 ggcccggcac ctgaacctgg cgggggagtt cgactgcgac cccgagccct tgcccacgct
     1261 ccgccactcc acctcgcacg tgatggcgca ggcggtgaag cggctctttc cccaggtgaa
     1321 ggtcgccatc ggccccgcca tcgaggacgg cttctactac gacttcctca aggcggagcc
     1381 gttcacgccc gaggatctcg cgcgcatcga ggacaccatg cgcgagatcg tcaagtccga
     1441 ctacccgttc acgcgggagg agatgccgcg agaccgggcc atcgcgttct tcgaggagcg
     1501 gggcgagccg ttcaaggtgg agatcctgcg agggctctcg gtgcccaccg tctcgatcta
     1561 ccgccagggc gacttcctcg acctctgccg cggcccgcac gtggcgtcga ccggccagct
     1621 caaggccttc aagctcctct cggcctccgg cgcctactgg cgcggcgacg agaagaaccc
     1681 gatgctgcag cggatctacg ggaccgcgtg gctcacccag gaggagctcg acaagcacct
     1741 ctggcgcctc gaggaggcga agaagcgcga ccaccgcaag ctcgggcggg agctcgacct
     1801 gttcgacttc tacgacatcg cccccggggc gccgttctgg ctgccgggcg ggatggtgct
     1861 ggtgcgcgag ctggagaagt acgcacgcga gtcgctcgac gcgcgcggct accaggagat
     1921 cgcgaccccg ctgctggtca acaagaagct ctgggagcag tccgggcact gggatctcta
     1981 ccaggagaac atgttcaagt tcgacgtcga gggcgacgtc ttcagcttga agccgatgaa
     2041 ctgcccggag tcggcctacg tgtacaagcg ggcgctgcgc tcctaccgcg acctgccgat
     2101 ccgctactcg gagatgggcc gctgccaccg caacgagcgg tcgggcgcgc tctccggcct
     2161 ggtgcgggtg cggcagttca cccaggacga cgcgcatctc tacgtccgcc ccgagcaggt
     2221 ccaggccgag atcaccgacc tgctcgacct ggtgcgcgag tggtacggca ctttcacgct
     2281 ccagccctcg taccgggtgg ccacccgccc gcccgacaag ctcgggaccg agcagcagtg
     2341 ggaccaggcc gaggacgcgc tccaccaggc cctccgcgcg aacgcgctcg tctacgagct
     2401 cgacaagggc ggcggggcct tctacgggcc caagatcgac atcgacgtgg aggacgcgct
     2461 cggccgccag tggcagctcg ccaccatcca ggtcgacctc acgatgctgc ccgagcgcat
     2521 gcagtgcgag tacatcgaca ccgacggcca ggccaagcgc ccggtggtga tccaccgcgc
     2581 gatcttcggc tcctacgagc ggttcgtggc gatcctcacc gagcacttcg cgggtgtgtt
     2641 cccgacgtgg ctcgctccgg tgcaggcgcg ggtcctcccg atcagcgaga agcacggcga
     2701 gtacggccgc aaggtgttcg cggcgctgcg ggccgcgcgc atccgcgccg aactcgacga
     2761 ccgcaacgag aagctgggct atcgcatccg cgacgcccag atccgcaagg tgcccta
//
LOCUS       DATMCV010001012         2309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_322311, whole genome shotgun sequence.
ACCESSION   DATMCV010001012 DATMCV010000000
VERSION     DATMCV010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322311"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VNU03_23210"
     CDS             complement(<1..390)
                     /locus_tag="VNU03_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWP77451.1"
                     /translation="MAKQILSCTVNGEAVEVLVQPYVTLLDALREDMGLTGPKEGCGT
                     GDCGACTVHVDGKVVASCLMLAMQVRGRKVRTIEGLAAADTTLHPLQDAFVRLGVPQC
                     GFCIPGVLMAAAALLEENPRPSEEEIRY"
     gene            complement(408..1268)
                     /locus_tag="VNU03_23215"
     CDS             complement(408..1268)
                     /locus_tag="VNU03_23215"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWP77452.1"
                     /translation="MRRFDLVLPNSLEDCLKILAERGAQAKLVAGGTDLLPQMKNGVL
                     KPAVVVDLSGIVRVRQVQNGSGLRIGAAVTARELELSPALRGAYLAIAESAAMVGSLQ
                     VRNLATVGGNLCNAAPSADMAPPLVALEAQAVIAGPKGERRVPMADFFTGVRQTVLAP
                     NEMLIELVVPPPGARSGGQYLRHTPRRELDIAVVGVASQLTIADGRCTKARIALASVA
                     PTPVRATAAERSLEGQAVTPEAIERAAGLAIEAARPIDDQRGSIEFRKHLVRVLTRRT
                     LTTALQRANA"
     gene            1479..>2309
                     /locus_tag="VNU03_23220"
     CDS             1479..>2309
                     /locus_tag="VNU03_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWP77453.1"
                     /translation="MMIRSIAGLWLLLTALLTCPTGAALAQSNVVILSTTTSTQDSGL
                     LDVLVPMFEKQSGLSVKTISVGTGQALALAARGEADVTLAHAPGVEKKYVEDGKMSNR
                     RLVMYNDFVIIGPPDDPAKIMGLPKAVDAFKRIAESQSRFVSRGDKSGTHVLEQGLWK
                     QVGLEPRGAWYIESGQGMGQTLGIANDRRAYTLTDRGTYLAFQKRVDLPILVEKDRPL
                     LNIYSVMEVNPANGPRVNVAGGKAFAEFMLAPETQAVIKAFGVDKYGQPLFVPVAGKK
                     D"
BASE COUNT          348 a          798 c          817 g          346 t
ORIGIN      
        1 gtagcggatc tcctcctcgg agggccgcgg gttctcctcc agcagggcgg cggccgccat
       61 cagcacgccc ggaatgcaga agccgcactg agggaccccg aggcggacga aggcgtcctg
      121 cagcgggtgg agcgtggtgt cggccgccgc caggccttcg atggtgcgaa ccttccggcc
      181 tcgcacctgc atggccagca tcaggcagct cgccacgacc ttcccatcga cgtggacggt
      241 gcaggcgccg cagtcgccgg tgccgcagcc ttccttggga ccggtgagac ccatgtcctc
      301 gcgcagagcg tcgaggaggg tcacgtaggg ctggacgagc acctcgacgg cctcgccgtt
      361 cacggtgcag ctgaggatct gtttggccac ggtggagagc ctccggatca ggcgttcgcg
      421 cgctgcagcg cggtcgtgag cgtgcgacgg gtgaggacgc ggaccagatg cttgcggaac
      481 tcgatggagc cgcgctggtc gtcgatgggc ctggccgcct cgatcgcgag accggccgcg
      541 cgctcgatgg cctccggcgt caccgcctgg ccttcgagcg accgctcggc cgcggtggcc
      601 cggaccggcg tcggcgcgac cgacgcgagc gcgatgcgcg ccttggtgca acggccgtcc
      661 gcgatggtca gctgggaggc cacccccacc accgcgatgt cgagctcgcg ccgcggcgtg
      721 tgccgcaggt actggccgcc gctgcgggcg cccggcggag gcacgaccag ctcgatcagc
      781 atctcgttcg gcgccagcac cgtctggcgc accccggtga agaagtcggc catcggcacg
      841 cgccgctcgc ccttcggccc cgcgatgacc gcctgggcct cgagcgccac cagcggcggc
      901 gccatgtcgg ccgacggcgc cgcgttgcag aggttgccgc ccacggtggc gaggttgcgc
      961 acctgcagcg agccgaccat ggccgcgctc tccgcgatgg ccagataggc cccacgcagc
     1021 gccgggctca gctcgagctc acgcgcggtg accgcggcgc cgatccggag gccgctcccg
     1081 ttctggacct ggcggacccg gacgatcccg gagagatcta cgacgacggc gggcttgagc
     1141 acgccgttct tcatctgggg caggaggtcg gtgcctcccg cgaccagctt ggcctgcgct
     1201 cctcgctcgg cgaggatctt gaggcagtct tcgaggctgt tcggcagaac gagatcgaat
     1261 cgtcgcatgt gttctccgtg gggtttggcg cgcgctcgcc atgatacacc gagtcgcggg
     1321 gagccggacg ggagtctact atgctgtcaa cagcatagtc aatgcgcctc ggccgcaccg
     1381 gccagccgcg ttgactatgc tcggaccagc ctgtatgctc gcgagcgcat attgaccttc
     1441 tgctccgccg agtcgctccc gcgtcacgga ggcgcgccat gatgattcga tcgatcgcgg
     1501 ggctctggct gctgctgact gccctgctga catgtccgac cggggcggcc ctcgcgcagt
     1561 ccaacgtcgt catcctctcg accaccacga gcacgcagga ttcgggcctg ctcgacgtgc
     1621 tggtgccgat gttcgagaag cagagcgggc tcagcgtgaa gaccatctcg gtcggcaccg
     1681 ggcaggcgct cgctctcgcg gcccgcgggg aggccgacgt cacgctggcc cacgccccgg
     1741 gggtcgagaa gaagtacgtc gaggacggca agatgagcaa ccgccgtctc gtcatgtaca
     1801 acgacttcgt gatcatcggc ccgcccgacg accccgcgaa gatcatgggc ctgccgaagg
     1861 cggtcgacgc cttcaagcgc atcgcggaga gccagtcgcg cttcgtctcg cgcggcgaca
     1921 agtccggcac ccacgtgctg gagcaggggc tgtggaagca ggtcggcctc gagccccggg
     1981 gcgcctggta catcgagtcc gggcagggca tgggccagac cctcggcatc gccaacgacc
     2041 ggcgcgccta caccctgacc gatcgcggga cgtacctcgc gttccagaag cgggtggacc
     2101 tgccgatcct cgtcgagaag gaccggccgc tgctgaacat ctactcggtg atggaggtga
     2161 acccggccaa cggcccgcgg gtgaacgtgg ccggcggcaa ggcgttcgcc gagttcatgc
     2221 ttgcgccgga gacccaggcg gtgatcaagg cgttcggggt ggacaagtac ggccagccgc
     2281 tcttcgtgcc ggtggccggc aagaaggac
//
LOCUS       DATMCV010001013         3089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_123781, whole genome shotgun sequence.
ACCESSION   DATMCV010001013 DATMCV010000000
VERSION     DATMCV010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123781"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VNU03_23225"
     CDS             complement(<1..417)
                     /locus_tag="VNU03_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003959880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77454.1"
                     /translation="MRLGLNIGYSRSQLGIDLSLVQHAERLGFHSVWSAEAYGSDAVS
                     PLCWIAARTERIRVGTAIMQIPARTPTLTATTALTIDQLSGGRFLLGLGVSGPQVVEG
                     WHGVAFGKPLAKTREYVDIVRMVWKRERPVEHKGEYY"
     gene            complement(429..776)
                     /locus_tag="VNU03_23230"
     CDS             complement(429..776)
                     /locus_tag="VNU03_23230"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWP77455.1"
                     /translation="MKRFHSAVYRLVERIPRGQVATYGQIAALLGYPRAARAVGQAMK
                     HVPAHLPWHRVVNAQGGISLRANVGSMLTQRVLLEQEGVVVRRGRIRLREHRWSGPRG
                     ARRPLAIAALARL"
     gene            complement(773..2581)
                     /locus_tag="VNU03_23235"
     CDS             complement(773..2581)
                     /locus_tag="VNU03_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77456.1"
                     /translation="MRDAEPEDEILGRAYDARVMRRVWSFTRPHIRLVLVTCALFPAV
                     SLIELAQPYLVKIAIDEHILRRDWAGLGRVALLFLLLLVALYVLRALQAYLTQLTGQR
                     VIHDLRAALFEHLQRQDARFYDRSPVGRLMTRLLNDGEAIQELFTSGLVSLLGDVVTL
                     TGVVVIMLGMNWKLALVTFALVPVLVIAAGYFRLRARDSYRVVRTRLARLNAFLQESL
                     QGMSVIQLFAREARERQMFGELSGALRRGQFRSTFYDASLYATVEALGAAAVALLLWY
                     GGGQVVTGALTFGGLVAFLEYTGRFFLPIRDLGAKYTVMQAATVSAERVFGLLDSRPT
                     ITSPPERALAPGAPAPRAATGDTPAVELRNVWFAYEGEQWVLRDCSLRMAPGEQVALV
                     GPTGEGKTTIARLLTRAYDVTRGQVLVDGVDVREWNLRTLRRHVGLIPQEVFLFSGTV
                     EDNLRLAGDGAVDRAAVERALAAASADRFVATLPRGLHEELRERGLNLSQGQRQLLAI
                     ARAIIYNPRVLALDEATSSIDPESEALVRVGIARLVEGRTSLVIAHRLSTIQRADRIL
                     VLHRGQIREAGRHAELLALGGLYSRLYELQFGLERT"
     gene            complement(2574..>3089)
                     /locus_tag="VNU03_23240"
     CDS             complement(2574..>3089)
                     /locus_tag="VNU03_23240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP77457.1"
                     /translation="RSVGYVPQESFLFSRPLRDNVRLADEHASPERLEEVARTAGLAD
                     DLGALPAGWNTVVGERGLTLSGGQRQRVALARALVANPPFLVLDDVLASVDAAKEWEI
                     TRALRAAASGRTTLLMTHRLKAAAEADRIVVLDEGRVVEEGRHAELVAAGGLYARLWR
                     VQQLEDELANA"
BASE COUNT          460 a         1146 c         1097 g          386 t
ORIGIN      
        1 gtagtactcg cccttgtgct cgaccggccg ctcgcgcttc cagaccatgc ggacgatgtc
       61 gacgtactcc cgcgtcttgg cgagcggctt gccgaaggcc accccgtgcc agccctccac
      121 cacctgcggg cccgagacgc cgaggcccag gaggaagcgg cctcccgaga gctggtcgat
      181 cgtcagcgcg gtggtggcgg tgagggtcgg ggtgcgggcc gggatctgca tgatcgcggt
      241 gccgacccgg atgcgctcgg tgcgggccgc gatccagcag agcggcgaga ccgcgtcgga
      301 gccgtaggcc tccgccgacc acaccgagtg gaagccgagc cgctccgcgt gctggaccag
      361 cgagaggtcg atgccgagct gggagcgcga gtagccgatg ttcagtccga gtcgcatagg
      421 tcctccgatc agagccgcgc gagggcggcg atggccagtg gacggcgcgc gccgcgcggg
      481 ccgctccacc ggtgctcccg gagccggatc cggccccgcc ggaccacgac gccctcctgc
      541 tccagcagca cgcgctgggt gagcatgctg cccacgttcg ccctcaggct gataccgccc
      601 tgcgcgttca ccacccggtg ccacggcagg tgcgccggca cgtgcttcat cgcctgcccc
      661 accgcgcgcg ccgcccgcgg gtacccgagg agcgccgcga tctggccgta ggtcgccacc
      721 tgccccctcg ggatgcgctc gaccagccgg tagaccgccg agtggaaccg cttcatgtcc
      781 gttccaggcc gaactgcagc tcgtacaatc gtgagtatag ccctccgagg gccagcagct
      841 ccgcgtgacg ccccgcctcc cggatctgtc cgcggtgcag cacgaggatc cgatccgcgc
      901 gctggatcgt cgacagccgg tgggcgatga cgaggctcgt cctcccctcc accaggcgcg
      961 cgatcccgac acgcaccagc gcttccgatt ccgggtcgat gctcgacgtc gcctcgtcca
     1021 gggcgagaac gcgaggatta taaatgatgg cacgcgcgat ggccagcagc tgccgctggc
     1081 cctgcgacag gttgaggccg cgctcgcgca gctcctcgtg gagtccgcgc ggcagcgtgg
     1141 ccacgaaccg atccgcgctc gccgccgcga gcgcccgctc caccgcggcc cgatcgaccg
     1201 ccccgtcgcc ggccagacgc aggttgtcct ccaccgtccc cgagaacagg aacacctcct
     1261 gggggatcag cccgacgtgg cggcgcagcg tgcgcaggtt ccactcgcgc acgtccaccc
     1321 cgtcgaccag cacctggccc cgggtgacgt cgtaggcgcg ggtgaggagc cgggcaatgg
     1381 tggtcttgcc ctcgccggtc gggcccacca gcgccacctg ctcgccgggc gccatccgca
     1441 acgagcagtc gcgcagcacc cactgctcgc cctcgtaggc gaaccacacg ttgcggagct
     1501 cgacggccgg cgtgtccccc gtggccgcgc gcggggccgg cgcgccggga gccagcgcgc
     1561 gctccggggg actcgtgatc gtcggccggc tgtcgaggag cccgaagacg cgctcggccg
     1621 acaccgtcgc cgcctgcatc acggtgtact tggctccgag gtcgcggatc gggaggaaga
     1681 agcgcccggt gtactcgagg aaggcgacca gcccgccgaa ggtgagcgcg ccggtgacca
     1741 cctgcccgcc cccgtaccag aggagcaggg ccaccgcggc ggcgcccagc gcctcgaccg
     1801 tcgcgtagag ggaggcgtcg tagaaggtgg agcggaactg cccgcggcgc agcgcgcccg
     1861 acagctcgcc gaacatctgg cgctcgcgcg cctcgcgcgc gaagagctgg atcacgctca
     1921 tgccctggag cgactcctgg aggaaggcgt tcaaccgggc gaggcgggtg cgcaccaccc
     1981 gatagctgtc gcgggcccgg agccggaagt agccggccgc gatcacgagc accgggacca
     2041 gggcgaaggt gacgagggcg agcttccagt tcatcccgag catgatgacc accaccccgg
     2101 tgagggtcac cacgtccccc aggagcgaga cgagcccgct cgtgaacagc tcctggattg
     2161 cctccccgtc gttgaggagc cgggtcatca ggcggccgac cgggctccgg tcgtagaagc
     2221 gggcgtcctg ccgctggagg tgctcgaaca gggccgcgcg gagatcgtgg atcacccgct
     2281 gcccggtgag ctgggtgagg taggcctgga gggcccgcag cacgtagagc gcgacgagca
     2341 ggagcaggaa gagcagggcc acccgcccca gcccggccca gtcgcggcgg aggatgtgct
     2401 cgtcgatcgc gatcttgacc aggtacggct gggccagctc gatgagcgac accgccggga
     2461 acagggcgca cgtgaccagg acgaggcgga tgtgcggccg cgtgaacgac cagacgcgcc
     2521 gcatgacgcg cgcgtcgtag gcccggccga ggatctcgtc ttccggctcg gcgtcacgca
     2581 ttggcgagct cgtcctccag ctgctgcacg cgccagaggc gcgcgtagag gccgcccgcg
     2641 gccaccagct cggcgtggcg cccctcctcc accacgcgcc cctcgtcgag caccacgatc
     2701 cggtccgcct cggccgcagc cttgagccgg tgcgtcatga gcagcgtggt gcgcccgctc
     2761 gccgccgcgc ggagcgcccg ggtgatctcc cactccttcg ccgcgtccac cgaggcgagc
     2821 acgtcgtcga ggaccaggaa cggcgggttc gcgaccagcg cgcgcgccag cgccacccgc
     2881 tggcgctggc caccggacag ggtgaggccg cgctccccca ccaccgtgtt ccagcccgcg
     2941 ggcagcgcgc ccaggtcgtc ggccaagccc gcggtgcggg ccacctcctc gagccgctcg
     3001 ggcgaggcgt gctcgtcggc cagccgcacg ttgtcgcgga gcggacggga gaagaggaag
     3061 gactcctgcg gcacgtatcc caccgagcg
//
LOCUS       DATMCV010001014         5636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_123794, whole genome shotgun sequence.
ACCESSION   DATMCV010001014 DATMCV010000000
VERSION     DATMCV010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123794"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VNU03_23245"
     CDS             <1..679
                     /locus_tag="VNU03_23245"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77458.1"
                     /translation="LEIAAGEFVSIVGPSGCGKTTLMKIVAGLLPCTRGEVRIDGVPV
                     HEPPDGVGMVFQTPTLLRWRTVLDNVLLPAAALRRPRARYEPVARDLIRLVGLEGFAN
                     HYPRELSGGMQQRVAISRALLLEPRLLLMDEPFGALDAMTREEMNLELQRIWNEQPKT
                     VLFITHSIDEAVFLSDRVVVMSPRPGGVRAVLDVELTRPRGLHVREHPRFVEYVGRIR
                     DLIFSKP"
     gene            690..2072
                     /locus_tag="VNU03_23250"
     CDS             690..2072
                     /locus_tag="VNU03_23250"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HWP77459.1"
                     /translation="MSSESHQSIRGFVSALERAYPDAVMRIAEPVDQAYQMQALALEL
                     ERRRRFPVLLFEQVRGHAIPVVSNVMASRRGLATALGVGEAELPETYAARLKEPVKPV
                     VLERPPFPVDVRRGDAVDLGSLPIPTYFPGDGGPYLTAGLLIARDPATGVATAGFHRF
                     QVKGPNRLGVSLHSRRRMFEFQQREEKAGRGLSCAIAIGLHPTVGMGSLSYPAPEISK
                     FEVVGGLLGEPMQLRRAETIDLDVPAWAEIVIEGEILPGEREPEGPFGEFTGYFSRRS
                     TDNIFVAKALVVREGAWFHSIASGRAPDHILPLGVLREAEIRNALRRVMPGVRAVHVP
                     TSGGASFTAYVSIKQTRPGEAKHAIPVVLGVDHYLKLVIVVDDDIDVFDESDVLWAIA
                     TRVQADRDLLVVGGSLGAILDPSASSDGLTAKLGIDATRRFGDAAAEKLVMTEAPMEW
                     ARRVVDRLGG"
     gene            2147..2629
                     /locus_tag="VNU03_23255"
     CDS             2147..2629
                     /locus_tag="VNU03_23255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77460.1"
                     /translation="MGVTARLLKQELLIHVDYGLPFGDIVVRYRGPISDPEALPEQVI
                     HLLRSRIPRDVQFELDELYQGFTRKIPPPSGPARVGWVIQPALATILIQDHERPTGLA
                     VAWLTYVQHVPASGPRLEQQHIALGAVDLSAEERTALERLRHRIKGIAWAHYQGPIGQ
                     "
     gene            2626..3255
                     /gene="pdxH"
                     /locus_tag="VNU03_23260"
     CDS             2626..3255
                     /gene="pdxH"
                     /locus_tag="VNU03_23260"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HWP77461.1"
                     /translation="MSEPLPEILPADPLPLAARWIGEAAAAVKSATAMALATVDGDGR
                     PTVRMVICRGFDAVAGSLVFYTDRESAKGVALERTPRAAVVFHWDVFERQIRVEGPVT
                     RVSEADSDRYWGTRPLDARLAAVASAQSHAIASRAAFLAQIEAARRAHGESVPRPPRW
                     GGYRIWAERVELWAGQPGRAHDRAAWTRAVQPTETGFTGGSWVGTRLQP"
     gene            complement(3290..4297)
                     /locus_tag="VNU03_23265"
     CDS             complement(3290..4297)
                     /locus_tag="VNU03_23265"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWP77462.1"
                     /translation="MYRAADAPPGALAGEAVAVLGYGHLGRTAALNLRDSGAKVRIGN
                     RDDEYAEQARSEGFEVVPIATAATGDVVYVLLPDEVIPTVFDATIAPVLRPGSAIAFG
                     SGYSLAFDLIHPPAGIDVLLVAPRMAGTTARSRYLEGQGFWACVGVEADASGRAQQRM
                     LGLADGLGVLRAGAVQMSAKMEATLDLFVEQTVGAVLGMAIMMAFEVAREAGIPGEAL
                     VLEMYMSGEMEAVFQAFRETGFFRASEDHGPTAVFGGITRSIEMDRDEMSDRFRRVLE
                     DIKSGGFARRFQDEAKSGYPMLEFARAMMHGGSPMGDAEDRIRRLTSSDGTGPAPAGA
                     A"
     gene            4408..>5636
                     /locus_tag="VNU03_23270"
     CDS             4408..>5636
                     /locus_tag="VNU03_23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWP77463.1"
                     /translation="MPGRPSAVTCCVAGGGPAGMMLAFLLARAGVPVMVLEKHTDFLR
                     DFRGDTIHPSTLELMHELGLLEEFLRVPHQKLPALVAQVGDTPLRVADFTHLPTRCKF
                     IAIMPQWDFLAFLSGHGARYPGFHLEMRAEATGLIEEAGRVIGLRGTAADGPFEVRAD
                     LVVAADGRSSILRERAGLQVEDLGAPMDVLWFRLSRSSGDPEATMGRFDVGRIFVLLN
                     RGQHWQCGYVIPKGSAEDVRRRGLDAFRASVAALIPFAADRVVAEIRDWDDVKLLTVR
                     VDRLRQWYRPGLLCIGDAAHAMSPVGGVGINLAIQDAVAAANRLWRPLREGRLTTDDL
                     RQVQRRREWPTRVTQWLQLLVQRRVIRTTLGRRGGRLTPPRLVRFLARFGPLARIPAR
                     LVGVGVRAEHVQTPERAA"
BASE COUNT          733 a         2019 c         2073 g          811 t
ORIGIN      
        1 gctcgagatc gcggcggggg agttcgtgtc gatcgtgggc ccgagcggct gcggcaagac
       61 cacgctgatg aagatcgtgg ccggcctgct cccgtgcacg cgcggagagg tccggatcga
      121 cggggtcccg gtgcatgagc cgcccgacgg ggtggggatg gtgttccaga ctcccaccct
      181 cctgcgctgg cgcacggtgc tggacaacgt gctgctcccg gccgccgcgc tgcggcgccc
      241 ccgcgcgcgg tacgagccgg tggcccggga cctgatccgc ctggtcggcc tcgagggatt
      301 cgcgaaccac tacccccgcg agctgtccgg cggcatgcag cagcgggtgg cgatcagccg
      361 ggccctgctg ctcgagccgc gcctcctgct gatggacgag ccgttcggcg cgctggacgc
      421 gatgacgcgc gaggagatga acctcgagct gcagcggatc tggaacgagc agcccaagac
      481 ggtgctcttc atcacccact ccatcgacga ggcggtcttc ctctccgacc gggtggtggt
      541 catgagcccg cggccgggcg gggtgcgggc ggtgctcgac gtggagctca cccgcccgcg
      601 cggcctccac gtccgcgagc acccgcgctt cgtcgagtac gtgggccgca tccgcgatct
      661 gatcttcagc aagccatagg agtcccgcca tgagcagcga atcccaccag agtatccgcg
      721 gcttcgtctc ggccctggag cgcgcgtatc ccgacgcggt gatgcgcatc gccgagccgg
      781 tggaccaggc ctaccagatg caggccctcg cgctcgagct cgagcggcgg cggcgcttcc
      841 cggtgctcct cttcgagcag gtgcgcgggc acgcgattcc ggtggtctcg aacgtgatgg
      901 cgagccgtcg cgggctcgcc accgcgctcg gggtcgggga ggcggagctg cccgagacct
      961 acgcggcgcg cctgaaggag ccggtgaagc cggtggtgct ggagcgcccg ccgttcccgg
     1021 tcgacgtccg ccgcggcgac gcggtcgacc tgggctcgct gccgatcccg acctacttcc
     1081 cgggcgacgg cggcccctac ctcaccgccg gcctcctcat cgcgcgcgac cccgcgaccg
     1141 gggtggccac cgcgggcttc caccgcttcc aggtgaaggg gccgaaccgc ctgggggtca
     1201 gcctgcactc gcgccggcgc atgttcgagt tccagcagcg ggaggagaag gcgggccggg
     1261 gcctctcgtg cgcgatcgcg atcgggctcc atcccaccgt cggcatgggc tcgctctcct
     1321 accccgcgcc cgagatctcg aagttcgagg tggtgggcgg gctcctcggc gagcccatgc
     1381 agctccgtcg ggcggagacg atcgacctgg acgtcccggc ctgggccgag atcgtgatcg
     1441 agggggagat cctgcccggg gagcgcgagc cggagggccc cttcggcgag ttcaccggct
     1501 acttctcgcg ccggagcacc gacaacatct tcgtggccaa ggcgctggtg gtgcgcgagg
     1561 gcgcgtggtt tcactccatc gcctccgggc gcgcccccga ccacatcctg ccgctcggcg
     1621 tgctccggga ggcggagatc cgcaacgccc tgcgccgggt gatgccgggg gtgcgggcgg
     1681 tgcacgtgcc cacctcgggc ggggcctcgt tcaccgccta cgtgtcgatc aagcagacgc
     1741 ggccggggga ggccaagcac gcgatcccgg tggtcctggg ggtcgatcac tacctcaagc
     1801 tggtgatcgt ggtggacgac gacatcgacg tgttcgacga gtcagacgtt ctgtgggcga
     1861 tcgcgacgcg ggtgcaggcc gaccgcgacc tgctggtggt cggcgggagc ctcggcgcaa
     1921 tcctggaccc gtcggccagc tccgacgggc tcaccgccaa gctcgggatc gacgcgaccc
     1981 ggcggttcgg ggacgccgcg gccgagaagc tcgtgatgac cgaggcgccg atggagtggg
     2041 cccgccgggt cgttgatcgg ctcggcgggt agccactcgg gccggcccgc ccgcacattt
     2101 cataccgtgg tgacgggggt catgcggaag aatacgtcca acgtcgatgg gcgtcaccgc
     2161 gcggctgctg aagcaggagc tgttgatcca cgtggactac gggttgccgt tcggtgacat
     2221 cgtcgtccgc taccgcgggc ccatcagcga cccggaggcg ctgccggagc aggtcatcca
     2281 cctcctgcgc agccggatcc cccgcgacgt ccagttcgag ctggacgagc tctaccaggg
     2341 cttcacgcgg aagatcccgc cgccgagcgg accggcccgg gtgggctggg tcatccagcc
     2401 ggcgctcgcc accatcttga tccaggatca cgagcgcccg accggcctcg cggtcgcgtg
     2461 gctcacgtac gtccagcacg tgcccgcgag cgggccccgc ctcgagcagc agcacatcgc
     2521 gctcggtgcg gtggacctca gcgcggagga gcgcaccgcc ctcgagcggc tccggcaccg
     2581 catcaagggg atcgcctggg cgcactacca ggggccgatc ggccagtgag cgagccgctc
     2641 ccggagatcc tgcccgccga ccccctgccg ctcgccgccc gctggatcgg agaggcggcg
     2701 gccgcggtga agagcgcgac cgcgatggcg ctggccaccg tcgacggcga cggccgcccg
     2761 accgtccgca tggtgatctg ccgcgggttc gacgcggtgg cgggctcgct cgtcttctac
     2821 accgaccgcg agagcgccaa gggggtggcg ctcgagcgga ccccgcgcgc ggcggtggtg
     2881 ttccactggg acgtgttcga gcggcagatc cgggtcgagg gcccggtcac ccgggtgtcg
     2941 gaggcggact cggaccgcta ctggggcacg cgcccgctcg acgcgcggct cgccgcggtc
     3001 gcgagcgcgc agagccacgc gatcgcctcg cgcgcggcgt tcctcgcgca gatcgaggcg
     3061 gcgcggcgcg cgcacgggga gagcgtcccg cggcccccgc gctggggcgg ctaccggatc
     3121 tgggccgagc gggtggagct gtgggcgggg cagccgggcc gcgcgcacga tcgtgcggcg
     3181 tggacgcgcg cggtgcagcc caccgagacc gggttcaccg gcggctcgtg ggtggggacg
     3241 cggctccagc cctgagaaga gcgcaccgcg agggtgcgcg gggcaaagct caggccgcgc
     3301 cggcgggcgc cggcccggtc ccgtcggacg aggtgagacg gcggatgcga tcctctgcgt
     3361 cgcccatcgg cgacccgccg tgcatcatgg cccgggcgaa ctcgagcatc gggtagccgc
     3421 tcttggcctc gtcctggaat cggcgggcga agcccccgct cttgatgtcc tccagtacgc
     3481 ggcggaagcg atccgacatc tcgtcgcgat ccatctcgat cgagcgggtg atgcccccga
     3541 acaccgcggt gggcccgtgg tcctcggacg cgcggaagaa gccggtctcg cgaaaggcct
     3601 ggaacaccgc ctccatctcg cctgacatgt acatctcgag caccagcgcc tcgccgggga
     3661 tgcccgcctc gcgcgcgacc tcgaaggcca tcatgatggc catgcccagc accgcgccca
     3721 ccgtctgctc cacgaacaga tcgagggtgg cctccatctt cgcgctcatc tgcaccgcgc
     3781 ccgcgcgcag cacgccgagc ccgtcggcca gccccagcat gcgctgctgc gcgcggccgc
     3841 tcgcgtcggc ctccacgccc acgcacgccc agaagccctg gccctcgaga taccgcgagc
     3901 gcgcggtggt gccggccatg cgcggggcca ccagcagcac gtcgatgccg gcgggcgggt
     3961 ggatgaggtc gaaggccagg ctgtagcccg agccgaaggc gatcgcgctg cccggccgga
     4021 gcaccggcgc gatggtggcg tcgaagacgg tggggatcac ctcgtcgggg agcagcacgt
     4081 acaccacgtc gccggtggcc gcggtcgcga tcggcacgac ctcgaagcct tcgctccgtg
     4141 cctgctcggc gtactcgtca tcgcgattgc cgatgcggac cttcgcgccg gagtcgcgca
     4201 ggttgagcgc ggcggtgcgc ccgaggtggc cgtagccgag gaccgctacc gcttcgccgg
     4261 cgagcgcgcc gggcggcgca tccgccgccc gatacacttg gaccggtgtc tctcccgggt
     4321 tcgtcattgc gtgatactaa cccatctctc gtggctttgc tgcgagttca ggcgtccggc
     4381 gggcggccat tgtagcatcg gggcatcatg cccgggcgac catcggcggt gacgtgctgc
     4441 gtggcgggcg gcggcccggc cgggatgatg ctcgctttcc tgctcgcccg ggccggcgtc
     4501 ccggtgatgg tcttggagaa gcacaccgac ttcctccgcg acttccgggg cgacacgatc
     4561 cacccctcga cgctcgagct catgcacgag ctgggcctgc tggaggagtt cctgcgcgtg
     4621 cctcaccaga agctgccagc cctcgtcgcc caggtcggcg acacgccgct gcgagtggcc
     4681 gatttcacgc accttccgac gcgctgcaag ttcatcgcca tcatgccgca gtgggacttc
     4741 ctcgccttcc tctcgggcca cggtgcccgc tatccgggct tccatctcga gatgcgggcc
     4801 gaggccaccg ggctgatcga ggaggcgggg cgggtgatcg ggctgcgcgg aaccgcggcg
     4861 gacgggccgt tcgaggtgcg cgccgatctc gtggtcgccg ccgacggccg ctcctcgatc
     4921 ctgcgcgagc gggccggcct gcaggtggag gacctggggg cgcccatgga cgtcctgtgg
     4981 ttccggctct cgcgctcgtc cggcgacccc gaggccacca tgggccgctt cgacgtgggg
     5041 aggatcttcg tactactcaa ccgggggcag cactggcagt gcggctacgt gatccccaag
     5101 ggctcggcgg aggacgtccg ccggcgcggg ctggacgcct ttcgcgcctc ggtggccgcg
     5161 ctcattccgt tcgcggcgga ccgggtggtc gccgagatcc gcgactggga cgacgtgaag
     5221 ctcctgaccg tccgggtgga ccgcctgcgc cagtggtacc ggccgggtct gctctgcatc
     5281 ggcgacgccg cgcacgcgat gtcgccggtc ggcggggtcg ggatcaacct ggcgatccag
     5341 gacgcggtgg ctgcggccaa ccggctctgg cggccgctcc gcgaggggcg gttgaccacc
     5401 gacgatctgc gccaggtgca gcgccggcgc gagtggccga cgcgggtgac ccagtggctg
     5461 cagctcctgg tgcagcgccg ggtcatcagg accacgctgg gtcgccgcgg cggccgcctc
     5521 accccgcccc ggctcgtccg cttcctggct cgcttcgggc cgctggctcg aatccccgcg
     5581 cggctggtgg gcgtcggcgt ccgcgccgag cacgtgcaga cgcccgagcg cgcggc
//
LOCUS       DATMCV010001015         4500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_460732, whole genome shotgun sequence.
ACCESSION   DATMCV010001015 DATMCV010000000
VERSION     DATMCV010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460732"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..587)
                     /locus_tag="VNU03_23275"
     CDS             complement(99..587)
                     /locus_tag="VNU03_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWP77464.1"
                     /translation="MAEHKATVRWALPADGEAFLKGRYSREHTWIFDGGMTVPASPAP
                     SVVPVPFSNPAHVDPEEAFVASIASCHMLTFLYVAMHAGFVVERYEDDAVGVMRKNER
                     GAIWVAAVTLHPRIVYGGAKRPTPAEVDHLHHLAHEQCFIANSVKTEITVADEDQSAG
                     RP"
     gene            complement(580..1200)
                     /locus_tag="VNU03_23280"
     CDS             complement(580..1200)
                     /locus_tag="VNU03_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HWP77465.1"
                     /translation="MSGREPRVLLGVGAAALLVSGIAPHDRLTWLLEVAPILIAVPVL
                     LATARRFPLTPLAYRLIFVHALILMLGGHYTYAEVPLGDWVADALGLARNPYDRLGHL
                     AQGFVPAIVVREILIRRSPLRPGKWLAFLVVCVCLAISAAYELVEWWSALIGGENADA
                     FLGTQGDQWDTQWDMFTALVGAIAALALLGRYHDRQIARLEEPNHG"
     gene            complement(1190..1699)
                     /locus_tag="VNU03_23285"
     CDS             complement(1190..1699)
                     /locus_tag="VNU03_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP77466.1"
                     /translation="MRLDMVRRELAQGAEIVTALSVGVSPEEATVRPAPGAWSLLEVI
                     GHLLDEERLDFRARLDLVLHRPDETWAAIDPEAWVIEHQYNAGDLAETLQGFLAERQR
                     SLAWLGTLTAPDWTREYRAPSGPITAGDLLASWAAHDLLHTRQLLELRRARLLGHTAP
                     HRTQYAGEW"
     gene            complement(1705..1935)
                     /locus_tag="VNU03_23290"
     CDS             complement(1705..1935)
                     /locus_tag="VNU03_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77467.1"
                     /translation="MASIRKHIDGGSLVVILITFVLFGVALFEKGLSHDLLLEAGVFL
                     VSVKLIIMSYKSALLSAQLKERLDEIHGALRR"
     gene            complement(1971..3149)
                     /locus_tag="VNU03_23295"
     CDS             complement(1971..3149)
                     /locus_tag="VNU03_23295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWP77468.1"
                     /translation="MFPLPPLTDEQRAVVERVAALSRDRFAARAARYDADSTFPYENY
                     ADLHAAGLHALAVPREYGGVGADSVTYVHALREIAKGCSATALTFNMHSTVTTFLAAL
                     ATEEQKRRYFGEVVGRGARIASITSEPEQSFRDKFVLNTVFRSTGDGGYRVAGVKQFC
                     SLGDAADYFFVTGILEGHASARDGVVSAMIPSRDAGVKIEGTWNATGMRGTISHTIRY
                     DCAVAAANVVGRPGQYLTIDLQGFALGYAAVYLGIGEAAFEYMTEMARTKTQRPSIEP
                     MSHHPLVQRTVAEVGTQIRAARLLLHEAACVKMAGDREATMLVVNQAKTYCADVGLMA
                     TERALRLAGGRGILKDLPLERWHRDSLAGPVMPPANDRCLETAGKLLCGLRAATLEFQ
                     "
     gene            3231..4082
                     /locus_tag="VNU03_23300"
     CDS             3231..4082
                     /locus_tag="VNU03_23300"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HWP77469.1"
                     /translation="MASSKRNLPVEAPARPDLLVTGLAAAGFAIAAYMTWLKWAGASA
                     AFCLSGSGCDVVQSSRYAMLLGVPTALWGAALDAAIGGLAAMGLGPRRWRWAFHLAAA
                     GIGVSVYLTALSLLVIHATCVYCLASTGLMLAIFLVLWWRRAALPGRRATIGALVAGG
                     LAVAVLAPFGAAFIFAMPSSAGSGSEAALARHLRESGAVMYGAYWCPHCQEQKALFGD
                     AARDLPYVECDPNGVNGRPDLCEKAGVKAFPTWTIGGQRREGVQSLGALADASKFQPP
                     PPPAASR"
     gene            complement(4098..>4500)
                     /locus_tag="VNU03_23305"
     CDS             complement(4098..>4500)
                     /locus_tag="VNU03_23305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HWP77470.1"
                     /translation="MSFGTLRREQMGGATGIYNLMRNLGGSVGISVATTLLSRRAQVH
                     QNVLVARMTPYDPVYQQKLEAIRQALVPKVGPHLAGQQAVGILYKTLVAQATLLAFVD
                     NFRLITVLAICSVPLVLLLKRVRARATPGAH"
BASE COUNT          612 a         1574 c         1653 g          661 t
ORIGIN      
        1 ctcttcgaca acagctcttc ggatgagccc tttcgtccgg ttgcggtgta tcgagacgga
       61 gcgttggtgt accgcaaggc tcctacgccc agttgggctc agggtcttcc ggcgctctga
      121 tcctcgtcgg ccaccgtgat ctcggtcttc accgagttgg cgatgaagca ttgctcgtgg
      181 gccaggtggt ggagatggtc cacctcggcc ggagtcgggc gcttcgcccc gccgtagacg
      241 atccgcgggt gcagcgtcac cgccgccacc cagatcgccc cgcgctcgtt cttccgcatc
      301 acccccaccg cgtcgtcctc gtagcgctcg accacgaagc ccgcgtgcat ggccacgtag
      361 aggaaggtca gcatgtggca gctcgcgatc gacgccacga aggcctcctc gggatccacg
      421 tgggccgggt tcgagaaggg caccggcacg accgacgggg cgggcgacgc ggggacggtc
      481 atgccgccgt cgaagatcca cgtgtgctcg cgagagtatc gccccttcag gaaggcctcg
      541 ccgtcggccg gcagggccca gcgcaccgtc gccttgtgct cagccatggt tgggctcctc
      601 cagcctcgcg atctggcggt cgtggtatcg gcccagcagc gccagggccg cgatcgctcc
      661 cacgagggcc gtgaacatgt cccactgagt gtcccactga tcgccctgcg tgcccaggaa
      721 ggcgtccgca ttctccccgc cgatcagggc cgaccaccac tcgaccagct cgtaggccgc
      781 gctgatggcc aggcacacgc agacgaccag gaacgcgagc cacttgccgg gccggagcgg
      841 cgaccggcgg atcaggatct cgcggacgac gatcgcgggc acgaagccct gggcgaggtg
      901 cccgaggcga tcgtaggggt tgcgggcgag cccgagcgcg tcggcgaccc agtccccgag
      961 cggcacctcg gcgtaggtgt agtgaccgcc cagcatcagg atcagggcgt ggacgaagat
     1021 caggcggtag gcgagcgggg tgagggggaa gcgtcgcgcg gtcgccagca gcacgggcac
     1081 cgcgatcagg atcggcgcga cctcgagcag ccacgtgagc cggtcgtggg gcgcgatgcc
     1141 ggagacgagc agcgcggccg cgccgacgcc caggaggacg cgcggctctc taccactcac
     1201 ccgcgtactg ggtacggtgc ggcgcggtgt ggccgagcag gcgggcccgg cgtagctcca
     1261 ggagctggcg cgtgtgcagg aggtcgtgcg cggcccagga ggcgagcagg tcgccggcgg
     1321 tgatcggccc ggacggggca cgatactcac gggtccagtc aggggcggtg agggtcccga
     1381 gccaggccag cgagcgctga cgctcggcca ggaagccctg cagggtctcg gcgagatccc
     1441 cggcgttgta ctggtgctcg atgacccagg cctcgggatc gatggcggcc cacgtctcgt
     1501 cgggccggtg cagcacgaga tccaggcgtg ctcggaagtc caggcgctcc tcgtcgagca
     1561 ggtggccgat cacctccagc agcgaccacg cccccggcgc gggccggacg gtcgcctctt
     1621 cgggggagac gcccaccgac agcgcggtca cgatctcggc cccctgcgcg agctcccgcc
     1681 ggaccatgtc gaggcgcatc gtggtcaccg gcggagcgcc ccgtggatct cgtcgaggcg
     1741 ctccttcagc tgcgcggaga gcagcgcgct cttgtacgac atgatgatga gcttcaccga
     1801 caccaggaac accccggcct cgagcagcaa gtcgtgggag aggcccttct cgaagagggc
     1861 caccccgaag aggacgaagg tgatcaggat gacgacgagc gagccaccgt cgatgtgctt
     1921 gcggatgctg gccatggatc ctcctgtgtc gtccgggggt acggcgcagc ctactggaac
     1981 tcgagagtgg ccgcgcgcag gccgcagagc agcttgcccg cggtctcgag gcagcggtcg
     2041 ttggcgggcg gcatcaccgg gccggccagc gagtcgcggt gccagcgctc gagcggcagg
     2101 tccttcagga tgccgcgccc gccggccagc cgcagggccc gctcggtggc catcaggccc
     2161 acgtccgcgc agtaggtctt ggcctggttc accaccagca tggtcgcctc gcggtcgccc
     2221 gccatcttca cgcaggccgc ctcgtgcagg agcagccgcg cggcgcggat ctgggtgccc
     2281 acctcggcca ccgtgcgctg caccagcgga tggtggctca tcggctcgat cgagggccgc
     2341 tgggtcttgg tgcgcgccat ctcggtcatg tactcgaagg cggcctcgcc gatgcccagg
     2401 tagaccgcgg cgtagccgag ggcgaagccc tgcaggtcga tggtgaggta ctggccgggg
     2461 cggcccacca cgttggcggc cgcgaccgcg cagtcgtagc ggatggtgtg gctgatggtg
     2521 ccccgcatgc cggtcgcgtt ccaggtgccc tcgatcttca cccccgcgtc gcggctcggg
     2581 atcatcgccg agacgacgcc gtcccgcgcg ctcgcgtggc cctccaggat gccggtgacg
     2641 aagaagtagt cggccgcgtc gccgagggag cagaactgct tgacccccgc cacccggtag
     2701 ccgccgtcgc cggtggagcg gaacacggtg ttgagcacga acttgtcgcg gaagctctgc
     2761 tcgggctcgc tcgtgatcga cgcgatgcgc gcgccccggc ccaccacctc gccgaagtag
     2821 cgccgcttct gctcctcggt ggcgagggcg gccaggaagg tggtcacggt ggagtgcatg
     2881 ttgaaggtga gcgcggtggc cgagcacccc ttggcgatct ctcggagggc gtgcacgtag
     2941 gtcaccgagt cggcgcccac gcccccgtac tcgcgcggga ccgcgagcgc gtgcaggccg
     3001 gccgcgtgca gatcggcgta gttctcgtag gggaacgtgg agtcggcgtc gtaccgcgcc
     3061 gcgcgcgccg cgaagcggtc gcgcgagagc gcggccacgc gctccaccac ggcgcgctgc
     3121 tcgtcggtga ggggcgggag cgggaacact ggcctgatct cctcctgggg gtcctggggg
     3181 gtcctggggt ccgggtcgcc cggcgcccct atggtactgt ggacgctgcc atggcgtcat
     3241 ccaagcggaa cctcccggtc gaggcgcccg cgcgccccga cctcctggtg accgggctcg
     3301 cggcggccgg gttcgccatc gcggcctaca tgacctggct caagtgggcg ggggcctcgg
     3361 cggccttctg cctgtcgggc agcggctgtg acgtcgtgca gtcgagccgg tacgccatgc
     3421 tgctgggagt gccgaccgcg ctctggggcg ccgcgctcga cgcggccatc ggggggctgg
     3481 ccgcgatggg gctcggcccg cggcgctggc gctgggcctt ccacctggcc gccgcgggca
     3541 tcggcgtctc ggtgtacctc accgcgctct cgctgctggt catccacgcc acgtgcgtct
     3601 actgcctcgc ctcgaccggc ctcatgctgg ccatcttcct cgtgctctgg tggcgccgcg
     3661 ccgccctgcc gggccggcgc gcgacgatcg gcgccctggt ggccggcggc ctcgccgtcg
     3721 cggtgctggc cccgttcggc gccgcgttca tcttcgccat gccgtcctcg gccgggagcg
     3781 gctcggaggc ggcgctcgcg cggcacctgc gcgagagcgg cgcggtcatg tacggggcct
     3841 actggtgccc ccactgccag gagcagaagg cgctcttcgg cgacgcggcc cgcgatctgc
     3901 cctacgtgga gtgcgatccc aacggggtga acgggcggcc ggacctctgc gagaaggcgg
     3961 gggtgaaggc gttcccgacc tggacgatcg ggggccagcg ccgcgagggc gtgcagtccc
     4021 tcggcgcgct cgccgacgcc tcgaagttcc agcctcctcc gccgcccgcg gcctcccggt
     4081 agcggtccgc cgggctctca gtgcgcgccc ggcgtggcgc gcgcgcggac ccgcttcagc
     4141 agcagcacca gcggcacgct gcagatcgcg agcaccgtga tcaaccggaa gttgtccacg
     4201 aaggcgagca gggtcgcctg cgccaccagc gtcttgtaga ggatgccgac cgcctgctgg
     4261 cccgccaggt ggggcccgac cttcgggacg agggcctgcc ggatcgcctc gagcttctgc
     4321 tggtagaccg ggtcgtaggg cgtcatccgc gccacgagca cgttctggtg cacctgcgcg
     4381 cgccgagaga gtagcgtggt ggccaccgag atgccgacgc tgccgcccag gttgcgcatc
     4441 aggttgtaga tcccggtggc gccgcccatc tgttcccggc gcagggtgcc gaacgacatg
//
LOCUS       DATMCV010001016         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_189773, whole genome shotgun sequence.
ACCESSION   DATMCV010001016 DATMCV010000000
VERSION     DATMCV010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189773"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VNU03_23310"
     CDS             <1..244
                     /locus_tag="VNU03_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWP77471.1"
                     /translation="ESSLTFDRREKASLYARARVEDYWIVNLVSRVLEVLRDPVADPT
                     APYGWRYARTRILTGVEVVFPVGAPLSRIPIADLLP"
     gene            328..>2528
                     /locus_tag="VNU03_23315"
     CDS             328..>2528
                     /locus_tag="VNU03_23315"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP77472.1"
                     /translation="MATERIAGFCALCRSRCGCVSVVEDGRLVAVEPDPSHPTGQHLC
                     VKGRAAPELVYAPDRLLYPMRRTRARNDPDPGWVRITWDEALDWTAERMRAIADREGP
                     EAVAFTVTTPAGTAISDGFPWINRLIRAFGSPNMVWGEELCAWHRDYATALTFGADIG
                     TPDFERTGCLLLWGHNPSSTYLPQATAVTEAAARGTALIVVDPRRAGPAARADQWLRV
                     RPGTDGALALGLASVMIAEGWYDRDFVRDWTNGPLLVREDTGRLLSAADLSPDGRPGD
                     HVAWDRASARPVAYDPVAGRFVERVVEPALTGPVACPTRGGGAIACVPAFEAYAALCR
                     RYPPERVERITGVPAAQVVETARLLWERRPVAYFHWTGLEQHTNATQTVRALSLLYAL
                     TGCWDAPGGNVRPARPAINDLAPLSLLSDKQRAKAIGLAERPLGPARQGWVTAGDLYR
                     AILHGTPYPVRGVVGFGANMLLSQPGATVARAAVDRLDFLVYADLFLTPTAAMADVVL
                     PVSSAWEREGLRVGFGPTQAGESFAQLRGPVIAPVGEARSDTWIVCELARRLGLGKSF
                     FDGSVDAGHRFALEPSGVTLEQLRAHPGGVRVPVEARFKRYTAPGPDGRSSGFATPSR
                     RVEIFSAQLAQHGQSPLPDYVEPAASPVSRPDLAARFPLRLTSAKVVQFCHSQHRSLP
                     RLRRHSPDPQAEMHPEAAKARGIAADDWVVVETPHGTMRARAGLNSSLDPEV"
BASE COUNT          294 a         1003 c          898 g          333 t
ORIGIN      
        1 ggagtcgagc ctcaccttcg atcgccgcga gaaggcgagc ctctacgccc gggcccgcgt
       61 ggaagattac tggatcgtca acctggtctc gcgcgtgcta gaggtcctcc gcgatccggt
      121 cgccgatccc accgcgccct acggctggcg ctacgcccgc acccgcatcc tgaccggggt
      181 cgaggtggtg ttcccggtcg gcgcgccgct ctcccgcatc ccgatcgccg acctgctgcc
      241 ctgacccggc ccttccgctc gccagcgtgg cgacctaggg cgccgcgcgc tcgcccggcg
      301 ccgccgagtc tgctaccgta ggcgcccatg gccacggagc gaatcgccgg cttctgcgcg
      361 ctgtgccgct cgcgctgcgg ctgcgtctcg gtggtggagg acggccggct ggtcgcggtg
      421 gagccggacc cgtcgcatcc caccgggcag catctgtgcg tgaagggccg cgcggcgccg
      481 gagctcgtct acgcccccga ccggctgctc tacccgatgc gccgcacgcg ggcccgaaac
      541 gatccggacc cgggctgggt gcgtatcacg tgggacgagg cgctcgactg gaccgcggag
      601 cgcatgcgcg cgatcgccga ccgggagggc cccgaggcgg tggccttcac cgtcacaacc
      661 cccgcgggca ccgcgatctc cgatggcttc ccgtggatca accggctcat ccgcgccttc
      721 ggcagcccca acatggtgtg gggcgaggag ctctgcgcgt ggcaccgcga ctacgccacc
      781 gcgctcacct tcggcgccga catcggcacg ccggacttcg agcgcaccgg ttgcctgctc
      841 ctctggggcc acaacccgtc gagcacgtac ctgccccagg ccaccgcggt cacggaggcc
      901 gccgcacggg ggacggccct gatcgtggtg gatccgcggc gcgcgggacc cgccgcgcgc
      961 gccgaccagt ggctgcgcgt gcggcccggc accgacgggg cgctggccct cggcctggcg
     1021 tcggtgatga tcgcggaggg ctggtacgat cgcgacttcg tccgtgactg gaccaacggc
     1081 ccgctgctgg tgcgcgagga cacgggccgc ctgctctcgg ccgccgacct ctcgcccgac
     1141 gggcgacccg gcgaccacgt ggcgtgggac cgcgccagcg cccggccggt cgcgtacgat
     1201 cccgtggccg gccggttcgt ggagcgcgtc gtcgagcccg cgctcaccgg cccggtcgcc
     1261 tgcccgacgc gcggcggagg cgccatcgcc tgcgtgcccg cgttcgaggc gtacgccgcc
     1321 ctctgccggc gctacccgcc ggagcgcgtg gagcggatca ccggggtccc cgccgcgcag
     1381 gtcgtcgaga ccgcgcgact gctctgggag cgccggccgg tggcctactt ccactggacc
     1441 ggcctcgagc agcacaccaa cgcgacccag accgtgcgcg cgctctcgct gctctacgcg
     1501 ctgaccggct gctgggacgc ccccggcggc aacgtccgcc ccgcgcgccc ggcgatcaac
     1561 gacctggcgc cgctctcgct gctctccgac aagcagcgcg cgaaggccat cggcctggcc
     1621 gagcggccgc tcgggcccgc gcggcaaggc tgggtgaccg cgggcgatct ctaccgcgcc
     1681 attctccacg gcacccccta cccggtgcgc ggggtggtcg gcttcggcgc gaacatgctc
     1741 ctctcgcagc cgggcgcgac ggtggcccgc gccgcggtcg accgcctcga cttcctcgtc
     1801 tacgcggatc tcttcctcac cccgaccgcg gcgatggcgg acgtggtgct gccggtctcc
     1861 tcggcgtggg agcgcgaggg gctgcgcgtc ggcttcgggc ccacccaggc gggcgagagc
     1921 ttcgcgcagc tgcgcgggcc ggtcatcgcg ccggtcggcg aggcgcgctc ggacacctgg
     1981 atcgtctgcg agctggcccg gcgcctcggc ctcggcaaga gcttcttcga cggcagcgtg
     2041 gacgccgggc atcgcttcgc gctcgagccg tcgggggtca cgctcgagca gctccgcgcg
     2101 caccccggcg gggtgcgggt gccggtcgag gcgcgcttca agcgctacac cgcgccgggc
     2161 ccggacggcc gctcgtccgg cttcgcgacc ccgagccgcc gcgtggagat cttctcggcg
     2221 cagctcgccc agcacggcca gagcccgctg cccgactacg tcgagcccgc cgcgagcccg
     2281 gtgagccggc ccgacctggc cgcgcgcttc cccctccgtc tcacctcggc caaggtcgtg
     2341 cagttctgcc acagccagca ccgaagcctg ccgcgcctgc ggcggcacag tcccgacccg
     2401 caggccgaga tgcaccccga ggccgcgaag gcccgcggca tcgcggccga cgactgggtg
     2461 gtggtcgaga ccccgcacgg caccatgcgc gcgcgagccg ggctcaacag cagcctcgat
     2521 cccgaggt
//
LOCUS       DATMCV010001017         3538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_656236, whole genome shotgun sequence.
ACCESSION   DATMCV010001017 DATMCV010000000
VERSION     DATMCV010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656236"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VNU03_23320"
     CDS             <1..495
                     /locus_tag="VNU03_23320"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HWP77473.1"
                     /translation="GDMVVGIAASGVTPFVRAALKEARRRGAGTALVTCNPDVPARAA
                     RVVIALAVGPEVLTGSTRLKAGTATKLALNTLTTASFARLGKVYGNRMVDLRPRSAKL
                     RARALRLVGQLGRVPPREAERLLKASGASAKVAIAMARLRIDAKEARRRLRQSAGSLR
                     RALE"
     gene            complement(503..1345)
                     /locus_tag="VNU03_23325"
     CDS             complement(503..1345)
                     /locus_tag="VNU03_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP77474.1"
                     /translation="MPSPSGFTDRFARVNGVRLHYVIGGKGSPVVLLHGYAETGHMWR
                     PIMPLLAQRHIVIVPDLRGAGGSAKPPAGYDKKNMAVDIHELTSSLGLDRVRIVGHDI
                     GLMVAYAYAAQFPQAAERVVLMDAFLPGIGNWKTMWLLRDLWHFHFYGDVPLALVKGR
                     ERIYFEHFWNDFAADRKRSVPEADRRVYAKAYAQPGGMRAGFEYFRSFERDAEDFARL
                     GQTRLAMPVSVMSGEKSGGTFLIDQVKLVATDVRGQVIPGSGHWLMEEAPKTVIPALL
                     DFLL"
     gene            1482..2189
                     /locus_tag="VNU03_23330"
     CDS             1482..2189
                     /locus_tag="VNU03_23330"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWP77475.1"
                     /translation="MGEDPRIVAAREKVRTIERMPPGTRPPPLLISGEPGTGKMSVAS
                     PFHHHIGPRRDRGMADGDWFGPGETHAEWMLFGNPAGYFHSMPRGTVLINWIERVPAR
                     LQTRLLETVWDPTLAWMIATTEVDLALEVRAGRFNRTLEEVLGASSIRLPPLRERGLD
                     VILLAEYILASWCIDNGLKPGALHLLPDAIDSLLRHPWPGNVGELEHVVWKAAFVARQ
                     GGIRAADLSLPPGNVGM"
     gene            complement(2206..2670)
                     /locus_tag="VNU03_23335"
     CDS             complement(2206..2670)
                     /locus_tag="VNU03_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77476.1"
                     /translation="MKAIGTLAAFALALAFPIAPAVSAPACPEVDSARAMLAKAAAAK
                     NEQDLKQMPRNVPAQPGPQAQPGAQTQPPGAQPKAGPQSQPKAGAQPAPDQPDQARSG
                     QDKAAPVVETAPVPEEMKRAAILVKEADEACQAGRAAQAAEKAQAAMALMKR"
     gene            3213..>3538
                     /locus_tag="VNU03_23340"
     CDS             3213..>3538
                     /locus_tag="VNU03_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWP77477.1"
                     /translation="MPVQLRVNGKKYSVEVDPETPLLWVLRDHLGLTGTRFGCGAALC
                     GSCTVHVDGEARRSCVLRVGEIGSGAVTTIEGLGARRPHPLQQAWIEHDVPQCGYCQA
                     GQIMQAA"
BASE COUNT          538 a         1196 c         1245 g          559 t
ORIGIN      
        1 ggcgacatgg tggtgggcat cgcggccagc ggggtgacgc ccttcgtgcg cgcggccttg
       61 aaggaggcgc ggcggcgcgg ggcgggcacc gcgctcgtca cctgcaaccc cgacgtgccg
      121 gcaagggcgg cgcgagtggt catcgcgctg gccgtcggtc ccgaggtgct gaccggctcg
      181 acgcgtctga aggcgggcac cgccaccaag ctcgcgctca acaccctgac caccgcgtcg
      241 ttcgcgcggc tcggcaaggt ctacggcaat cgcatggtgg acctgcgccc acgctcggcc
      301 aagctgcgcg cccgggcgct gcgcctggtc ggccagctcg gccgtgtgcc gccccgcgag
      361 gccgagcgcc tcttgaaggc ctccggcgcc agcgccaagg tcgccatcgc catggcccgc
      421 ctccgcatcg acgccaagga agcgcgccgc cgcctgaggc aatccgccgg ctccctccgc
      481 cgcgccctcg agtaggccgg ggtcagagga ggaagtcgag gagggcgggg atcacggtct
      541 tgggggcttc ctccatcagc cagtggccgg agcccgggat cacctggccg cggacgtcgg
      601 tggccaccag cttcacctgg tcgatcagga aggtgccgcc cgacttctcg cccgacatca
      661 ccgagaccgg catcgcaagc cgggtctgac caaggcgggc gaagtcctcc gcgtcccgct
      721 cgaaactgcg gaagtactcg aagccggcgc gcatccctcc cggctgcgcg taggccttcg
      781 cgtagacgcg acggtcggcc tccggcaccg agcgcttccg gtcggccgcg aagtcgttcc
      841 agaagtgctc gaagtagatg cgctcgcgcc ccttcaccag cgccagcggc acgtctccgt
      901 agaagtggaa gtgccacagg tcgcgcagaa gccacatggt cttccagttg ccgatcccgg
      961 gcaggaaggc gtccatgagc accacgcgct cggcggcctg cgggaactgc gcggcgtagg
     1021 cgtaggcgac catcaacccg atgtcgtgcc cgacgatgcg cacgcgatcg agcccaaggg
     1081 acgaggtcag ctcgtggatg tccaccgcca tgttcttctt gtcgtagccg gccggcggct
     1141 tggccgatcc tccggccccg cgcaggtcgg gcacgatcac gatgtggcgc tgggccagga
     1201 gcggcatgat cggccgccac atgtgcccgg tctccgcgta gccgtgaagc aggacgaccg
     1261 ggctgccctt gccgccgatg acatagtgca gccggacccc gttgaccctg gcgaagcgat
     1321 cggtgaagcc gctcgggctt ggcatggggc gagcatgggc ccgcaccgag gcagcgtcaa
     1381 gcggacgcat agtatcttgg gagaatggcg tcgttcgatg aggttatgcg atcagcgaga
     1441 agcaccgcca agtctttggg agtgactacg tcgccctgtt catcggcgag gatccgcgga
     1501 tcgtagccgc gagggagaag gtgcggacga tcgaacggat gccgcccggc acccgtccgc
     1561 ccccgctgct gatctccggt gagcccggga ccgggaagat gagcgtcgcg agcccgttcc
     1621 accaccacat cgggcctcga cgagatcggg ggatggccga cggggattgg ttcgggccgg
     1681 gcgaaacgca cgcggagtgg atgctgttcg gcaaccccgc ggggtatttc cattcgatgc
     1741 cccggggtac cgtgctcatc aactggatcg agcgagtgcc tgcgcgtctg cagacacggc
     1801 tgctcgaaac cgtgtgggat cccacactcg cctggatgat cgcgaccact gaggtcgatc
     1861 ttgcgctcga ggtccgggcg ggtcgcttca accggacact cgaggaggtc ctgggcgcct
     1921 cgtcgatccg cctgccgcca ttgcgcgagc gcgggctcga cgtcatcctc ctggccgaat
     1981 acattctcgc gagctggtgc atcgacaatg gactgaagcc tggcgctctc catctgcttc
     2041 cggatgcgat cgattcgctc ctgaggcacc catggcccgg gaacgtcggt gaactggaac
     2101 atgtcgtgtg gaaggcggca ttcgtggcga ggcaaggcgg aatccgcgcg gccgacctgt
     2161 ccctgccacc cggaaatgtc ggaatgtaag acctgacccc aatctctagc gcttcatgag
     2221 ggccatcgcg gcctgggctt tctccgcggc ctgggcggcc cggccggcct ggcacgcctc
     2281 atcggcttcc ttcacgagga tcgccgcgcg cttcatctcc tccggcaccg gcgcggtctc
     2341 gaccaccggc gctgccttgt cctggccgct gcgcgcttga tccggctgat ccggcgcggg
     2401 ctgggcgccc gctttcggct gggactgcgg gccggccttg ggctgcgctc ccggcggctg
     2461 cgtctgggca cccggctgcg cctgcgggcc cggctgcgcc ggcacgttgc gcggcatctg
     2521 cttcaggtcc tgctcgttct ttgcggccgc cgccttggcg agcattgccc gggccgagtc
     2581 gacctccggg cacgcggggg ccgagacggc cggggcgatc gggaacgcca gtgccagggc
     2641 gaaggctgcc agagtcccga tggctttcat cgcaaggatc tcctccggca ggagctagat
     2701 cgcatcgacc gtgccagggc agaaagtggg cgaatcccag gcgatgaagg gcgaagtccg
     2761 gctcgcggtc acgcgagccg gtcaggagga cgcagtaatg ctttcgctcc gggggcgtgc
     2821 ctgaggcccg acgctcgggg ccacgcttca ccccgggcgc cgggcccatc gtctgcgaag
     2881 ccgcggcgtg cttacggcag cggcggaaga gaaggcaccg gcggcggggc ggccacgctc
     2941 gggatcctcc cccaccgggg gaccccgtca ccacgtggct cgtatcggcc gtgcgggttg
     3001 atcaatgtca tcgcacggac tatgacgcag gcgcccgccg ggttatttac accggcttgc
     3061 cgagatcagc ccttgacccc acccatagtc agcccggcgg tcatgcgccg gcggaaggcg
     3121 tcgtggatcg cgatcggcgg cagcgccttg cggcaagaga ctgctcgcga taccctgacg
     3181 gtcttcagat aatgtgacgg gcgatcaact cgatgccagt gcagcttcgc gtcaacggca
     3241 aaaaatacag cgtcgaggtc gatccggaga ctccgctgct gtgggtcttg cgggatcatc
     3301 tgggtctcac cggaacgcgc tttggatgtg gcgccgcgct ttgcggcagc tgcaccgttc
     3361 acgtggacgg cgaggcccgg cgttcctgcg tgctgcgcgt gggcgaaatc gggagcggcg
     3421 ccgtcacgac gatcgagggg cttggggcgc ggcggccgca tcccctccag caggcgtgga
     3481 tcgagcatga cgtgccccaa tgtggatatt gccaggcggg ccagatcatg caggcggc
//
LOCUS       DATMCV010001018         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_521956, whole genome shotgun sequence.
ACCESSION   DATMCV010001018 DATMCV010000000
VERSION     DATMCV010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521956"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VNU03_23345"
     CDS             complement(<1..526)
                     /locus_tag="VNU03_23345"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HWP77478.1"
                     /translation="MIRRSFIKIGGDLAIDFANTEAPQGDREDSLRSWRDLIDFLELR
                     GAVSRDEGVGLRAMGERDAHACAAAFERALELRAIVRAMLEALAARRPLQAGWVAAVN
                     QALGWGTGASRLVRQGRGWRLAFASTLGEPPRALAPIARSVADLAAQGRAVAVRKCAN
                     PRCILYFRDTSRTRR"
     gene            632..1285
                     /locus_tag="VNU03_23350"
     CDS             632..1285
                     /locus_tag="VNU03_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HWP77479.1"
                     /translation="MRVAELWRYPVKSMGGERLDEALIGEGGIAGDRVVHVSGRGGRI
                     ITSRTHPRLLGLHGHLGADGEPLVDGLPWSAPESAAAVRAAAGAEARLVRYEGPERFD
                     VLPLLVGTDGAFEQLGVDHRRLRPNIVIGGVDGLAERGWPGQRLRIGEVVISVAKLRA
                     RCVMTTYDPDTQVQDLSVLRRIASEFGGQMALDCDVVSGGRIAVGDPVELLEGKAEA"
     gene            complement(1282..1863)
                     /gene="pdxT"
                     /locus_tag="VNU03_23355"
     CDS             complement(1282..1863)
                     /gene="pdxT"
                     /locus_tag="VNU03_23355"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWP77480.1"
                     /translation="MKIGVLALQGDFDMHRKVLDRIGVASVEVRMPRQLDDVAGLIMP
                     GGESTTLLKLMDAWDFVPAIEKFNAAGKPIFGTCAGMILLAREVQSPAQFSLGLIDIT
                     VERNAYGRQKESFEAVGSADLEQGPRPLKMVFIRAPRIRRLGAAVTPLAEHRGECVMA
                     RQGTVMVGAFHPELTDEAAVHEYFAGMVRAAGG"
     gene            complement(1860..2489)
                     /locus_tag="VNU03_23360"
     CDS             complement(1860..2489)
                     /locus_tag="VNU03_23360"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWP77481.1"
                     /translation="MESRKSPDLHQLPANLPVPVDDGACDHLPGSRVPAVRLSSTSGR
                     WVDLVEISDRPTVLFFYPRTGEAGRSAGPEWDAIPGARGCTPQSCGFRDLHREFVALG
                     VTVFGVGTQETAYQQEFVERNHVPFEMLSDCDLALTRALRLPTFEYAATRGGPPTLIK
                     RMAWYVERGCIERIWYPVFPPDRNAETVLGWLRERGRLLSIDRGEVRLT"
BASE COUNT          376 a          903 c          911 g          371 t
ORIGIN      
        1 ggcggcgcgt gcgggaggta tcgcggaaat agaggatgca gcgcgggttc gcgcacttcc
       61 ggacggccac ggcccggccc tgcgctgcca ggtcggccac ggacctcgcg atcggggcca
      121 gcgcccgggg cggctcgccc agggtcgagg cgaaggcgag tcgccagccg cgtccctgcc
      181 ggacgagccg gctcgcgccg gtgccccagc ccagcgcctg gttcaccgct gccacccacc
      241 cggcctgcag cgggcgccgg gccgcgaggg cttccagcat cgcgcggacg atcgctcgca
      301 gctcgagcgc ccgctcgaag gccgccgcgc aggcgtgcgc gtcccgttcc cccatggcgc
      361 gcaggcccac accctcgtcc cggctcaccg cgccccgcag ctcgaggaaa tcgatcaggt
      421 cccgccagga gcggaggcta tcctcccggt ctccctgagg cgcctcggtg ttggcgaaat
      481 cgatcgccag atcgccaccg atcttgatga acgaccttcg gatcatgatg gtaaggtctc
      541 gtaattacgt gttgatctag aaagctatcc cggccgaatc gactaacctg tttctatgat
      601 ctaagaggtt agcacgagag gaggcagcgc gatgcgggtt gcggagctct ggcggtaccc
      661 ggtgaagtcg atgggcggag agcggctgga cgaggccctg atcggcgagg gcggaatcgc
      721 gggcgaccgc gtggtgcacg tgagcgggcg gggcggccgc atcatcacgt cgcgcaccca
      781 cccgcgcctg ctgggactcc acgggcacct gggggcagac ggcgagccgc tggtcgatgg
      841 gctcccctgg agcgcgcccg agagcgcggc ggcggtccgg gccgcggccg gcgcggaggc
      901 gcgcctggtc cgctacgagg ggccggagcg cttcgacgtg ctgcccctgc tggtcggcac
      961 cgacggcgcg ttcgagcagc tcggggtcga ccaccgtcgg ctccgcccga acatcgtcat
     1021 cggtggcgtg gacgggctgg cggagcgcgg ctggccgggc cagcggctcc ggatcggaga
     1081 ggtcgtcatc tcggtggcga agctccgggc ccggtgcgtc atgaccacgt acgacccgga
     1141 cacccaggtc caggacctgt cggtgctgcg acggatcgcc tccgagttcg gcggtcagat
     1201 ggcgctcgac tgtgacgtgg tgagcggggg ccggatcgcg gtgggcgacc cggtcgagct
     1261 gctggaggga aaggcggagg cctaaccgcc ggcggcgcgc accatcccgg cgaagtactc
     1321 gtggaccgcg gcctcgtcgg tcagctcggg atggaaggca ccgaccatga cggtgccctg
     1381 gcgggccatg acgcactcgc cgcggtgctc ggccagtggc gtcaccgcgg ccccgagccg
     1441 gcggatgcgg ggcgcccgga tgaagaccat cttgagcggc cgcgggccct gttcgaggtc
     1501 ggccgagcca accgcctcga aggactcctt ctggcggccg taggcgttgc gctccaccgt
     1561 gatgtcgatc aggcccaggg agaactgggc cgggctctgc acctcgcggg ccagcaggat
     1621 catgccggcg caggtgccga agatgggctt gccggccgcg ttgaacttct cgatcgcggg
     1681 cacaaagtcc caggcgtcca tcaacttcag cagcgtggtg ctctccccgc cgggcatgat
     1741 cagccccgcg acgtcgtcga gctgacgcgg catgcgtact tccacggagg ccaccccgat
     1801 acgatcgagg accttccggt gcatgtcgaa gtctccctgc agggccagga cgccgatctt
     1861 cacgtgagcc tcacctctcc ccgatcgatg gacagcagcc ggcctcgctc tcgaagccac
     1921 ccgagcacgg tctccgcgtt ccggtccggc gggaagaccg gataccagat ccgctcgatg
     1981 catccccgct ccacgtacca ggccatgcgc ttgatcaggg tgggcggccc gccccgtgtg
     2041 gccgcgtact cgaaggtcgg cagccgcagc gcgcgggtca gcgcgagatc gcagtcgctc
     2101 agcatctcga acggcacgtg attgcgctcc acgaactcct gttggtacgc ggtctcctgg
     2161 gtgccgaccc cgaagaccgt cacgcccagc gcgacgaatt cccggtgcag gtcgcggaat
     2221 ccgcagctct ggggcgtgca gccccgcgcg ccgggaatgg cgtcccactc gggcccggcc
     2281 gaacgcccgg cctcgccggt cctcgggtag aagaagagca cggtcggccg gtccgagatc
     2341 tccaccaggt ccacccaccg gccggaggtc gacgacagcc ggacggccgg cacccgcgac
     2401 cccggaaggt ggtcgcaggc gccgtcgtcg accggcaccg gcaggtttgc cgggagctgg
     2461 tggagatcgg gactcttcct gctctccatc gttagctccc ctcttgtatc cgctcggctc
     2521 gccttcggct gcgcctcgcc aagcgactgc cccttaccag c
//
LOCUS       DATMCV010001019         4260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_591771, whole genome shotgun sequence.
ACCESSION   DATMCV010001019 DATMCV010000000
VERSION     DATMCV010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591771"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VNU03_23365"
     CDS             <1..706
                     /locus_tag="VNU03_23365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77482.1"
                     /translation="DCVQVEAVPPDRPEALPPGPYAVLRVMDTGVGMDAATQARIFEP
                     FFTTKEPGKGTGLGLSMVHGVVRQHGGAVQVRSAVGGGATFEIYLPQVATPVEPARIT
                     ETAGRETAGRETILLAEDEDDVRALTREVLERQGYRVLEASDGLQALRLYEAEGDRID
                     LVLSDVVMPRMSGRELVDRVRAIRADMRVLYMSGYTEDAIVRHGVRDASTVLLGKPFA
                     PADLLAKIREVLDRVR"
     gene            complement(728..2113)
                     /locus_tag="VNU03_23370"
     CDS             complement(728..2113)
                     /locus_tag="VNU03_23370"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWP77483.1"
                     /translation="MRRPPPGFRWLIPLVGCLAVLAAPTPAGLTPEGQRVLAVIVLAI
                     GLWGLEAVPSGVTAIVAIVALVGTGAVAGIREGLAGFADPIAFFLVGVLTMGAAVARS
                     GLAERIAHQLLAHGRGRAGALYLHLLLAMPLLTLLLPSASARTGILVHVYDQALELGR
                     VPRGAPLARAVMMLLNSVNRLSSTILLTGGITPIVAAGLIGTISWTRWFVLMSVPYAV
                     LLALAALAIYLRYRRGFRARITVPAAPARRPFTGVEWRTALITGGAGLLWLTDAFHHL
                     HPTLPALIAWVCFLAPGIGVLTWREFEQEVGWTNFFVIGASLSLAQGLARSGASDWLA
                     GGVVGAAHGVAASPVMVVVVLLLASSVVRLMIPNITGFLATTIPVAMSVGTAAGLNPL
                     LCGLIVTIAGDAVLYYPAQSASSLVVYERGHLGAGEIFRFGLLMTALGAVVVLAVALP
                     YWTLIGEPLTP"
     gene            complement(2126..2290)
                     /locus_tag="VNU03_23375"
     CDS             complement(2126..2290)
                     /locus_tag="VNU03_23375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77484.1"
                     /translation="MRLLLLIPLAALLLAGCAIVPIGPYAYAPPRPRVSYVYPAYPYG
                     WYAPRYYGHW"
     gene            complement(2399..3592)
                     /locus_tag="VNU03_23380"
     CDS             complement(2399..3592)
                     /locus_tag="VNU03_23380"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP77485.1"
                     /translation="MLQGIRVLDLSRVIAGPYSAALLGDLGADVIKLERPGRGDDMRS
                     FRGDGSMSAAFAAVNRNKRGIAVDLQKPEGARLAFELARRADVVVENFLPGVAERLGL
                     GYPAIRAVNPAVVYASVTGFGQTGPLARKPGYNTIAQGMSGLMALTGMPGDPPTRVGG
                     SVSDVAASYMAFGMVNAALVRRFRTGEGQHVDVSLVSASLGLLPDPVAIYFDTGERPV
                     RVGNRNLNLSPAEAFRAGDGFVNVVLMNPDQWGRFCGALGDGAMETDPRFVTNTARLE
                     NHPAFKARVEAILGTGSAKEWVARLEAAGIAAGPIYEFHEVFEDPQVRHLGLVAEVEQ
                     PGAGKVKMLTFPGRASATPPRIDRPAPLLGQHTAEVLGELGLSREEIDRLAAAGVIVL
                     GEQPA"
     gene            3758..>4260
                     /locus_tag="VNU03_23385"
     CDS             3758..>4260
                     /locus_tag="VNU03_23385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWP77486.1"
                     /translation="MRYQRISADCHIDLPWIPPDLFTANASAELKDRMPYVKDGPDGP
                     FWTSRNGTSFGLWGGVGPAGSKYEAGKHHRVDVMAATGLYADGKKGVARPTTPALRAK
                     DMDRDGVQAEVIYGILGAATRLNDHEAATEMFHIYNDWLVDFCGHDPDRFIGLACLPY
                     GDIDAAV"
BASE COUNT          686 a         1520 c         1542 g          512 t
ORIGIN      
        1 cgactgcgtc caggtcgagg cggtcccgcc ggaccggccg gaggccctgc cgcccggccc
       61 gtacgcggtg ctgcgagtca tggacaccgg cgtggggatg gacgcggcca cgcaggcacg
      121 catcttcgaa cccttcttca ccaccaagga gcccggcaag ggcaccgggc tcgggctctc
      181 gatggtgcac ggggtggtgc gccagcacgg gggcgccgtc caggtgcgca gcgcggtggg
      241 cggcggcgcc acgttcgaga tctacctgcc tcaggtggcg actccggtcg agcccgcccg
      301 gatcacggag accgcgggcc gcgagaccgc cgggcgcgag accatcttgc tggcggagga
      361 cgaggacgac gtgcgcgcgc tcacccgcga ggtgctggag cggcagggct accgcgtgct
      421 cgaggcgagc gacgggctgc aggccctccg actttacgag gccgaaggcg accgcatcga
      481 tctcgtcctc tccgacgtgg tgatgccccg catgagcggg cgcgagctgg tggatcgcgt
      541 gcgcgccatc cgggccgaca tgcgcgtgct gtacatgtcc ggctacaccg aggacgccat
      601 cgtccggcac ggggtgcgcg acgcgagcac ggtgctgctc ggcaagccgt tcgccccggc
      661 cgatctgctc gccaagatcc gcgaagtcct cgatcgcgtc cgctagcccg cctctctcag
      721 ccgccggcta gggggtcagc ggctccccga tcagggtcca gtacggcagc gccaccgcca
      781 gcaccaccac cgcgcccagc gcggtcatca ggagcccgaa gcggaagatc tcccccgccc
      841 cgaggtgacc ccgctcgtac accaccaggg acgacgcgct ctgcgccggg tagtagagga
      901 ccgcgtcgcc cgcgatagtg acgatgagcc cgcacagcaa cgggttgagc cccgccgcgg
      961 tgcccaccga catcgccacc ggaatggtgg tggccaggaa gccggtgatg ttcgggatca
     1021 tcagccgcac caccgacgag gccagcagca gcaccaccac caccatgacc ggcgacgccg
     1081 ccacgccgtg agccgccccc accacgccac ccgcgagcca gtcgctggcg ccggagcgcg
     1141 cgagtccctg ggccagcgag agggaggccc cgatgacgaa gaagttggtc cagcccacct
     1201 cctgctcgaa ctcccgccag gtcagcacgc cgatgccggg cgccaggaag cagacccagg
     1261 cgatcagagc gggcagcgtc gggtgcaggt ggtggaacgc gtcggtgagc cagagcagcc
     1321 ccgcgccccc ggtgatgagc gcggtgcgcc actccacccc cgtgaacggg cgacgcgcgg
     1381 gcgcggcggg caccgtgatg cgcgcgcgga agccgcgccg atagcgcagg tagatcgcga
     1441 gcgcggcgag cgcgagcagc accgcgtacg gcacgctcat gaggacgaac cagcgcgtcc
     1501 acgagatcgt gccgatgagg ccggcggcca cgatgggcgt gatgccgccg gtcaatagaa
     1561 ttgtggagga gagccggttg accgagttca gcagcatcat caccgcgcgc gcgagcggcg
     1621 ccccgcgcgg cacgcggccg agctcgaggg cctggtcgta cacgtggacg aggatgccgg
     1681 tgcgcgcgct ggccgaggga agcagcagcg tgaggagcgg catcgccagg agcaggtgga
     1741 gatagagcgc gccggcccgc ccgcggccgt gggccagcag ctgatgcgcg atccgctccg
     1801 cgaggccgct ccgggccacc gccgcgccca tggtgagcac cccgaccagg aagaacgcga
     1861 tcggatcggc gaacccggcg aggccctcgc ggatgcccgc gaccgcaccc gtgccgacca
     1921 gggccacgat ggcgacgatc gcggtgacgc cggaaggcac ggcctcgagc ccccagagcc
     1981 cgatggccag cacgatcacg gccaggacgc gctggccctc gggggtgagg cccgcggggg
     2041 tgggcgccgc cagcaccgcc aggcatccca ccagcgggat gagccagcgg aagccgggcg
     2101 gcgggcggcg cacccgccga ggatactacc agtgaccgta gtagcggggc gcgtaccagc
     2161 cgtacgggta cgccggatac acatagctca cgcgcggtcg gggcggggcg tacgcgtacg
     2221 ggccgatcgg aacgatggca caaccggcca gcagcagcgc ggccagtggg atgagcaaga
     2281 gcagtcgcat gatcgtgtcc tcctcgctga tcagacgggc gaggcgggcg cgcctattcc
     2341 tgctttgatt ttgcttttga ggcgtaaacg ccgggcgggc cggctacgtc taacgaagct
     2401 aggccggttg ctcgcccagg acgatcaccc cggcggcggc gaggcggtcg atctcctcgc
     2461 gcgagagtcc gagctcaccc agcacttccg cggtgtgctg gccgaggagc ggagccgggc
     2521 gatcgatgcg cggcggcgtg gcggaggcgc ggcccgggaa ggtcagcatc ttcaccttgc
     2581 cggcgccggg ctgctccacc tcggccacca gccccaggtg acgcacctgc gggtcctcga
     2641 acacctcgtg gaactcgtag atcggtcccg ccgcgatgcc ggccgcttcc aggcgggcca
     2701 cccactcctt cgcggaaccg gtgccgagga tggcttcgac ccgcgccttg aacgcgggat
     2761 ggttctcgag ccgcgcggtg ttggtgacga agcgcggatc ggtctccatc gcaccgtcgc
     2821 cgagcgcgcc acagaaacga ccccactggt cggggttcat cagtaccacg ttgacgaatc
     2881 cgtcgccggc ccggaaggcc tccgcgggcg agagattcag gttgcggttg cccacgcgca
     2941 ccggccgctc gccggtgtcg aagtagatcg cgaccggatc gggcaggagc ccgaggctcg
     3001 ccgacaccag gctcacgtcg acgtgctggc cctccccggt gcggaagcgg cgcaccagcg
     3061 ccgcgttgac catgccgaac gccatgtagg acgcggccac gtcggagacc gagccgccca
     3121 cccgcgtcgg cggatcgccg ggcatgccgg tcagcgccat cagcccgctc atgccctgcg
     3181 cgatggtgtt gtagccgggc ttgcgcgcga gcgggccggt ctggccgaag ccggtgaccg
     3241 aggcgtagac caccgcgggg ttcaccgcgc ggatcgcggg gtagcccagc ccgagccgct
     3301 cggccacgcc gggcaggaag ttctcgacca ccacgtcggc gcggcgggct aactcgaagg
     3361 ccagccgcgc gccctcgggc ttctgcaaat ccaccgcgat cccgcgcttg ttgcggttca
     3421 ccgccgcgaa ggcggcgctc atcgagccgt cgccgcggaa ggagcgcatg tcgtcgccgc
     3481 ggccgggccg ctcgagcttg atcacgtcgg cgcccaggtc gcccagaagg gccgccgagt
     3541 agggccccgc gatcacgcgg gagaggtcga ggacgcggat gccctggagc atggcagccc
     3601 gggcatggtg ccgcggcggc gggcccgagt caaggcgcct gcggccggcc cgccggccgc
     3661 gccgcgggga cgacccccgg cttgacgagg cccccgccgg tggctaggct ctgtccacac
     3721 gcgagagatg agcgattcac cgaccaaagg gggcgtcatg cgctaccaac ggatctccgc
     3781 cgactgccat atcgacctgc cctggatccc acccgatctg ttcaccgcga acgcgtcggc
     3841 cgagctcaag gaccgcatgc cctatgtgaa ggacggcccc gacgggccct tctggacctc
     3901 caggaacggc acgagcttcg ggctctgggg cggcgtgggg ccggccgggt ccaagtacga
     3961 ggccggcaag caccaccgcg tggacgtgat ggcggcgacc ggtctctacg ctgacggcaa
     4021 gaagggcgtc gcccggccaa cgactccggc gctacgggcg aaggacatgg accgcgacgg
     4081 ggtgcaggcg gaggtcatct acggcatcct cggggcggcc acccggctga acgaccacga
     4141 ggcggccacc gagatgttcc acatctacaa cgactggctc gtggacttct gcggccacga
     4201 tcccgaccga ttcatcggcc tggcctgcct gccctacggc gacatcgacg cggcggtgca
//
LOCUS       DATMCV010001020         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_993580, whole genome shotgun sequence.
ACCESSION   DATMCV010001020 DATMCV010000000
VERSION     DATMCV010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993580"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VNU03_23390"
     CDS             <1..446
                     /locus_tag="VNU03_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811285.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HWP77487.1"
                     /translation="SEIVVDFYDRLKSISKGYASFDYEFLEFRASDMVKLDILLNGDP
                     VDALSIIVHREKAYEKGKALAEKLRAVIPRQLYEVAIQAAIGSRVIARETVKAMGKNV
                     TAKCYGGDITRKRKLLEKQKEGKKRMKQVGRVEVPQEAFLSVLKS"
     gene            456..1127
                     /gene="lepB"
                     /locus_tag="VNU03_23395"
     CDS             456..1127
                     /gene="lepB"
                     /locus_tag="VNU03_23395"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWP77488.1"
                     /translation="MNDLQVRPERDAVPAAPVVKVRRKSLFREYSEAIIIAVLLALFI
                     RQFAVQAFTIPSGSMMDTLLIGDYILVNKFLFGAEIPLTDLHLPGVRDPRRGDIIVFK
                     YPNDETRDFIKRIIAVGGDTVQVRDNRVTLNGHLIDEPYARPGSFSGQPSTACGYAYA
                     CEPLKVPDGSYFVMGDNRDNSQDSRYWGFVRREKIRGKAFLIYWSWNGDGHWLRWRRL
                     GQYLP"
     gene            1166..>2030
                     /gene="hemW"
                     /locus_tag="VNU03_23400"
     CDS             1166..>2030
                     /gene="hemW"
                     /locus_tag="VNU03_23400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWP77489.1"
                     /translation="MHLPFCVERCGYCSFNTAPYTPGGQDRFLAALLREWDLFHAAPW
                     VGGVRLDTLFLGGGTPSLLTVDQMATLLARIDARFAVEPGAEVTVECNPDDVTRERVA
                     GYRRAGVTRVSLGVQSLDDAILPILDRRHSARQARAAFVAAREAGADNISVDLIYGLP
                     GLDLATWTATVREVIAWGPDHLSAYGLTLDEGSLWHARGVAGLPPEEAVTAQYWALAE
                     AAAEAGFEHYEVSNYARPGFRSRHNQRYWRWEEYLGLGPGACGFLGHVRYANVKPVER
                     YTTLVEAGQLPL"
BASE COUNT          318 a          715 c          686 g          311 t
ORIGIN      
        1 tgagcgagat cgtggtggac ttctacgacc gtctcaagtc catctcgaag ggctacgcgt
       61 ccttcgacta cgagttcctg gagttccggg cctcggacat ggtcaagctc gacatcctcc
      121 tcaacggcga cccggtggac gcgctctcga tcatcgtcca ccgcgagaag gcctacgaga
      181 agggcaaggc gctcgccgag aagctgcgcg cggtgatccc gcgccagctc tacgaggtcg
      241 cgatccaggc cgccatcggc agccgggtca tcgcgcgcga gacggtcaag gccatgggca
      301 agaacgtcac cgccaagtgc tacggcggcg acatcacgcg gaagcgcaag ctgctcgaga
      361 agcagaagga aggcaagaaa cgcatgaaac aggtcggtcg agtcgaggtc ccgcaggagg
      421 cgttcctgtc ggtcctgaag agctgagcgc ggggcatgaa cgaccttcag gttcgtcccg
      481 aacgtgacgc ggtgccggcg gcgccggtgg tcaaggtgcg ccggaagtct ctcttccgtg
      541 agtattccga agcgatcatc atcgcggtgc tgctggctct cttcatccgc cagttcgcgg
      601 tgcaggcctt cacgatcccg tccggctcca tgatggacac gctgctgatc ggcgactaca
      661 tcctggtgaa caagttcctc ttcggggccg agatcccgct caccgacctg catcttcccg
      721 gggtccgcga tccccgccgc ggcgacatca tcgtcttcaa gtaccccaac gacgagacgc
      781 gcgacttcat caagcgcatc atcgcggtgg gcggcgacac cgtccaggtg cgcgacaacc
      841 gggtgaccct gaacggccac ctgatcgacg agccgtacgc gcgaccgggc tcgttctccg
      901 gacagccctc gaccgcctgc ggctacgcct acgcgtgcga gcccctcaag gtgcccgacg
      961 ggtcgtactt cgtcatgggg gacaaccgcg acaattcgca ggacagccgc tactggggct
     1021 tcgtccggcg agagaagatc cgcgggaagg ccttcctgat ctactggtcg tggaacgggg
     1081 acgggcactg gctgcgctgg cgccggctgg gtcagtacct gccgtagcgt cgctccggcc
     1141 ccgggccgcc cggctcgggg cctacgtgca cctgccgttc tgcgtcgagc gctgcgggta
     1201 ctgctcgttc aacaccgcgc cctacacccc gggggggcag gatcgcttcc tcgcagcact
     1261 cctccgcgag tgggacctgt tccacgccgc gccctgggtg ggcggcgtca ggctcgatac
     1321 cctgttcctc ggcggcggca cgccctcgct cctcaccgtc gaccagatgg ccacgctgct
     1381 ggcccggatc gacgcccgct tcgcggtgga gccgggcgcc gaggtgacgg tcgagtgcaa
     1441 cccggacgac gtgacccggg agcgcgtggc cggctaccgc cgggccgggg tcacccgggt
     1501 gagcctcggc gtgcagagcc tggacgacgc gatcctgccg atcctggacc ggcgccattc
     1561 cgcccggcag gcccgcgccg ccttcgtggc cgcgcgggaa gcgggagcgg acaacatcag
     1621 cgtcgacctg atctacggcc tgcccggcct cgacctcgcg acgtggaccg ccaccgtgcg
     1681 cgaggtgatc gcgtggggcc cggaccacct gtccgcctac ggcctcaccc tggacgaggg
     1741 cagcctctgg cacgcgcgcg gcgtggcggg actcccgccc gaggaggcgg tcaccgcgca
     1801 gtactgggcc ctcgcggagg cggccgcgga ggccggcttc gagcactatg aagtctccaa
     1861 ctacgcgcgg cccggcttcc gctcgcgcca caaccagcgc tactggcgct gggaggagta
     1921 tctcgggctc ggaccgggcg cgtgcggctt cctcggccac gtgcgctacg ccaacgtcaa
     1981 gccggtggag cgctacacga cgctcgtcga ggcggggcag ctcccgctcg
//
LOCUS       DATMCV010001021         4353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_396986, whole genome shotgun sequence.
ACCESSION   DATMCV010001021 DATMCV010000000
VERSION     DATMCV010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396986"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VNU03_23405"
     CDS             <1..428
                     /locus_tag="VNU03_23405"
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWP77490.1"
                     /translation="ILAAAGLRRLGLAPPHLTLLPVDEFVPAVGQGILAVEAREADRE
                     VLELLGRLDDTRSRSEAVAERAFLDRLGADCHTPVAGHARHDGSALTLTGIVASLDGG
                     TVLRSQATGGPRQAEQLGASVAEELLAKGAKALLDAGRG"
     gene            484..1233
                     /locus_tag="VNU03_23410"
     CDS             484..1233
                     /locus_tag="VNU03_23410"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWP77491.1"
                     /translation="MTRAREQAGAFAELLEAAGARVLLVPTIAIEPPESWAPLDTALA
                     GDFAWVIFTSVNGVSMVRRRVVATGQGRALLERARLAAIGPATAAALAGWDLRSAVVP
                     DEYVAEALVERLRPLIAPGGRVLLPRAAETRDLLVRELTALGAAVTEVPAYRTRAATE
                     QAPGLREALAEGRVDVVTFTSSSTVRSFCALFGAAELPRLMAGVTVACIGPITRATAQ
                     GRGLVPRIVPEDYTIPGLARAIVAHFDPARS"
     gene            1237..2220
                     /gene="hemB"
                     /locus_tag="VNU03_23415"
     CDS             1237..2220
                     /gene="hemB"
                     /locus_tag="VNU03_23415"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWP77492.1"
                     /translation="MPFPIYRPRRLRESPLLRSMVRETTLRAADFVYPLFAVHGRGVR
                     DPIGSMPGQYRLSIDELLKECKDAASMGIPAVLLFGLPRDKDPRGTEAYAEDGIIQQA
                     VRAVKDTIPDLLVITDVCLCEYTSHGHCGVVEDGRVKNDPSLELIARTAISHAEAGAD
                     MVAPSDMMDGRVAAIREGLDEGGFTETPIMAYSAKYASAFYGPFREAADSTPQFGDRR
                     SYQMDPANALEAMREVALDVDEGADIVMVKPALPYLDVIARVKGEFGLPVAAYSVSGE
                     YAMLKAAGQLGWLDEERAMIEALTGIRRAGADIIITYFAKDAARLIEQGRA"
     gene            2217..2663
                     /locus_tag="VNU03_23420"
     CDS             2217..2663
                     /locus_tag="VNU03_23420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWP77493.1"
                     /translation="MRISAKGEYAIKAMVDLALHDGPDLQPIQDIAARQSIPQRYLEQ
                     VLLQLKRSGFLYARRGSSGGYRLSRPPDQITVGALLRAVEGPSAAADGPQRGGRGAAP
                     DDSAGLGELWREIDAAVAAVVDRTSLEDLRRRADERRSAARPMYHI"
     gene            2707..3651
                     /gene="cysK"
                     /locus_tag="VNU03_23425"
     CDS             2707..3651
                     /gene="cysK"
                     /locus_tag="VNU03_23425"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HWP77494.1"
                     /translation="MTTKKATAPARPRLAGSVLELIGATPLVRLNRLPRAGGATVLAK
                     MESLNPGGSVKDRIALAMVEDAERRGVLAAGATLVEPTSGNTGIGLAMVCAVRGYRLI
                     LTMPDDMSVERQRLLARYGAEIHLTPAMEGMTGAVFAAQEICREHPDYFMPLQFENPA
                     NPEVHRRTTALEILEATDERIDVFVAAVGTGGTVTGVGEVLKERVPALRVIAVEPARS
                     PVLSGGRFRPHGIQGIGASFVPGILNRSILDEIVTVRDEDATATARRLAREEGLLVGI
                     SSGANVWAACRIAESLPAGQVVVTVLCDTGERYLSVTF"
     gene            3662..4018
                     /locus_tag="VNU03_23430"
     CDS             3662..4018
                     /locus_tag="VNU03_23430"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWP77495.1"
                     /translation="MSVQVYIPTPFRRATKNADRVAVEAPDVKALLDELEDQFDALKG
                     LVRNDQGEVHHHVNIYVNNEAIEALQGLDTTLKDGDEVSIIPALAGGGPALAGGGPAL
                     AGGGPALAGGEPALAG"
     gene            4015..>4353
                     /locus_tag="VNU03_23435"
     CDS             4015..>4353
                     /locus_tag="VNU03_23435"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HWP77496.1"
                     /translation="MILTGDELVRVHAQAVAEYPAECCGVVLVRDAPTAERVLMPCRN
                     IQDELHDKDPQRHPRNSRTAYFIDPKDLLAIGRREAQGYRVATIYHSHIDTGAYFSPT
                     DRQNALIDGEP"
BASE COUNT          600 a         1575 c         1588 g          590 t
ORIGIN      
        1 ccatcctggc cgcggccggc cttcgccggc tgggcctcgc cccgccgcac ctcaccctgc
       61 tcccggtcga tgagttcgtg cccgcggtgg gccaggggat cctggccgtc gaagcccgcg
      121 aagccgaccg ggaagtgctt gaacttctgg ggcggctgga cgacactcgg agcaggtcgg
      181 aggccgtggc ggagcgggcg ttcctcgacc ggctcggggc cgactgccac acgccggtgg
      241 ccggccacgc gcgccacgac gggtcggcgc tgaccctgac cgggatcgtg gcgagcctcg
      301 acggcggaac ggtgctccga tcgcaggcga ccggtggccc ccggcaggcc gagcagctcg
      361 gggcgtccgt ggcggaggag ctcctggcaa agggggcaaa ggcactgctc gatgcgggcc
      421 gcgggtgatc aggtggtgac tgaggtagag cgtggtaagc cattgaccgg gcgggtggtt
      481 ctggtgacgc gtgcgcgtga gcaggccgga gcttttgccg agctgctcga ggccgccggg
      541 gcccgggttc tcctggtccc caccatcgcg atcgagccac ccgagtcctg ggccccgctc
      601 gacacggccc tcgcgggcga cttcgcgtgg gtgatcttca ccagcgtgaa cggggtgagc
      661 atggtgcgcc ggcgcgtcgt cgccaccggc caagggcgcg cgctcctgga gcgcgcccgg
      721 ctcgccgcca tcggtcccgc caccgcggcg gcgctcgccg ggtgggacct ccggtcggcg
      781 gtggtgccgg acgagtacgt ggccgaggcc ctcgtcgagc gcctgcggcc cctgatcgcg
      841 ccgggcgggc gcgtgctcct gccgcgcgcc gcggagacgc gcgacctgct cgtgcgtgag
      901 ctgaccgcgc tcggcgcggc ggtgaccgag gtgcccgcgt accgcacccg cgccgcgacc
      961 gagcaggcgc ccggactccg cgaggcgctg gcggagggtc gcgtggacgt ggtcacgttc
     1021 accagctcgt ccaccgtgcg gagcttctgc gcgctcttcg gcgcggccga gctgccgcgg
     1081 ctgatggcgg gggtcaccgt tgcgtgcatc ggccccatca cccgggccac cgcgcagggg
     1141 cgggggctcg tgccgcggat cgtgccggag gactacacca tcccgggcct cgcccgcgcc
     1201 atcgtggccc acttcgatcc cgcgaggagc tagcgcatgc cgttccccat ctaccgcccc
     1261 cgccgccttc gcgagtctcc gctgctgcgc tcgatggtgc gcgagacgac gctgcgcgcc
     1321 gccgacttcg tctatccgct cttcgcggtc cacggccgcg gggttcgcga tcccatcggg
     1381 tccatgcccg gacagtaccg gctctcgatc gacgagctgc tcaaggagtg caaggacgcc
     1441 gcgtcgatgg ggatccccgc ggtgctcctg ttcgggctgc cgcgcgacaa ggacccgcgc
     1501 ggcaccgagg cctacgcgga ggacggcatc atccagcagg cggtgcgcgc ggtgaaggac
     1561 accatccccg acctgctggt gatcaccgac gtgtgcctgt gcgagtacac gagccacggc
     1621 cactgcgggg tggtggagga cgggcgggtg aagaacgatc cctcgctcga gctgatcgcg
     1681 cggaccgcga tctcgcacgc cgaggccggg gcggacatgg tcgcgccctc cgacatgatg
     1741 gacggccgcg tggcggccat ccgggagggg ctcgacgagg gaggcttcac cgagacgccg
     1801 atcatggcct actcggccaa gtacgcttcc gcgttctacg gcccgttccg cgaggcggcc
     1861 gactccaccc cccagttcgg cgaccgccgc tcctaccaga tggaccccgc caacgcgctg
     1921 gaggcgatgc gggaggtcgc gctggacgtg gacgagggcg ccgacatcgt gatggtgaag
     1981 ccggcgctgc cctacctcga cgtcatcgcc cgggtgaagg gcgagttcgg cctgccggtg
     2041 gcggcctact cggtgtccgg cgagtacgcc atgctcaagg cggccggcca gctcggttgg
     2101 ctcgacgagg agcgcgcgat gatcgaggcc ctgaccggga tccgccgcgc cggggccgac
     2161 atcatcatca cctacttcgc caaggacgcg gcccggctca tcgagcaggg tcgggcgtga
     2221 ggatctcggc gaagggcgag tacgccatca aggcgatggt cgatctggcc ctgcacgacg
     2281 gccccgacct ccagcccatc caggacatcg cggcgcgcca gtcgatcccg cagcgctacc
     2341 tcgagcaggt gctgctccag ctcaagcgga gcggctttct ctacgcgcgg cgcggctcct
     2401 cgggcggcta ccggctgagc cggccgcccg accagatcac ggtgggggcg ctcctgcggg
     2461 cggtcgaggg accgtccgcc gcggccgacg ggccccagcg cggtgggcgc ggcgcggccc
     2521 ccgacgactc ggccgggctc ggcgagctgt ggcgggagat cgacgccgcg gtggccgcgg
     2581 tggtcgatcg cacctcgctc gaggacctgc gccggcgcgc cgacgagcgg cgcagcgcgg
     2641 cccgccccat gtaccacatc tgacgactcc tcacccgaca cccgcgcttc cccacaggac
     2701 ttgcccatga cgaccaagaa agcgaccgcg cccgcccggc cccggctggc cggctcggtc
     2761 ctcgagctga tcggcgcgac ccctctggtg cggctcaacc gtctcccgcg ggcgggcggc
     2821 gccaccgtgc tggccaagat ggagtcgctg aacccggggg gcagcgtcaa ggaccggatc
     2881 gcgctcgcga tggtggagga cgcggagcgc cgcggcgtgc tcgcggcggg cgccacgctg
     2941 gtggagccga ccagcggcaa caccggcatc gggctcgcga tggtgtgcgc ggtgcgcggc
     3001 taccgcctca tcctcaccat gcccgacgac atgagcgtgg agcggcagcg gctgctcgcc
     3061 cgctacggag ccgagatcca cctcaccccc gcgatggagg gcatgaccgg cgcggtcttc
     3121 gcggcgcagg agatctgccg cgagcatccc gactacttca tgccgctcca gttcgagaac
     3181 ccggccaacc cggaggtgca ccggcgcacc accgcgctcg agatcctcga ggcgaccgac
     3241 gagcggatcg acgtgttcgt ggccgcggtc ggcaccggcg gcaccgtgac cggcgtcggc
     3301 gaggtgctga aggagcgcgt gcccgcgctg cgggtgatcg cggtcgagcc ggcccgctcg
     3361 ccggtgctct cgggcggacg cttccggccc cacggcatcc agggcatcgg ggcgtcgttc
     3421 gtgcccggca tcctcaatcg ctcgatcctc gacgagatcg tgacggtgcg cgacgaggac
     3481 gccaccgcga cggcccgccg tctcgcccgc gaggagggcc tgctcgtggg catctcgtcg
     3541 ggggccaacg tgtgggccgc gtgccggatc gcggaatcgt tgcccgccgg ccaggtggtg
     3601 gtcacggtgt tgtgcgatac tggcgagcgc tatctgagcg tcaccttcta ggagagcggc
     3661 catgagtgtc caggtctaca ttcccacccc gttccgccgc gccaccaaga acgccgaccg
     3721 cgtcgcggtc gaggcgcccg acgtcaaggc gctgctcgac gagctcgagg atcagttcga
     3781 cgcgctgaag gggctcgtcc gcaacgacca gggcgaggtg caccaccacg tcaacatcta
     3841 cgtgaacaac gaggcgatcg aggcgctgca ggggctcgac accacgctga aggacgggga
     3901 cgaggtctcg attattccag ccctcgcggg cggcgggcca gccctcgcgg gcggcgggcc
     3961 agccctcgcg ggtggcgggc cagccctcgc aggtggcgag ccagccctcg cgggatgatc
     4021 ctcaccggcg acgagctcgt ccgggtgcac gcgcaggcgg tggccgagta cccggctgag
     4081 tgctgtgggg tcgtgctggt gagggacgcg ccgaccgccg agcgggtcct catgccgtgc
     4141 cgcaacatcc aggacgagct ccacgacaag gatccgcagc ggcatccgcg caactcccgg
     4201 accgcctact tcatcgaccc gaaggacctg ctcgccatcg gccggcgcga ggcgcagggc
     4261 tatcgggtcg ccaccatcta ccactcccac atcgacaccg gcgcctactt ctcgcccacc
     4321 gaccgccaga acgcgctgat cgatggagag ccg
//
LOCUS       DATMCV010001022         5086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_190124, whole genome shotgun sequence.
ACCESSION   DATMCV010001022 DATMCV010000000
VERSION     DATMCV010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190124"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            564..1757
                     /locus_tag="VNU03_23440"
     CDS             564..1757
                     /locus_tag="VNU03_23440"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWP77497.1"
                     /translation="MTSALPLEGLRVLELGHIIAGPSAGLLLADLGADVIKIERPGEG
                     DQSRGMPAGTSANFHFLNRNKRSIAIDLKGSEEARALFLRLVAGSDVVIDNFAHGAVE
                     ALGLGYDVLTRTNPGIIYLALKGFLPGPHEARPFLDELAQMSAGLAFMTGPRGQPLRA
                     GASIVDVGAAAYGVVAVLAALHQRAETKRGQKITSGLFETTVFWVGQWLAQYAATGQP
                     SVPMPEIRQGTRMGWGVYQLFTGADGEQVFIGITSNGHWERFCAEFGLDDLGADPRLD
                     DNAKRVAARDWLPGRIAQEMARHSSAELSDRLEKARVPYAPLRRPDQLVDDPHLNASG
                     QFMITPLPDRPPAKLPKLPVHSTAFEMELRRPAPRLGEHTREVLAELGLDKDEIDALA
                     SRRVI"
     gene            1754..2734
                     /locus_tag="VNU03_23445"
     CDS             1754..2734
                     /locus_tag="VNU03_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HWP77498.1"
                     /translation="MSDMPRHVHIKEVGPREGFQFEKGDITLAAKISLVDALSDTGVG
                     TIEFTSFVSPKWVPQMADAEQMVAGIKRVPGVAYEAIWLNEAGLERAARLRESLALRP
                     MFGVAATDTFTRKNTNRSVDEKVAELPGWARRYQALGMDWIDAGVMAAFGCNYEGDVT
                     PAQVIGVIEKVEAQAVGCGSRLGNISLADTMGWANPLQIKRLVGAVRARWPEIPVKLH
                     LHDTRALAIANVVAALEMGVDRFDSAVAGLGGCPFAAHKGAAGNVTTEDLVFLCQELG
                     VDTGIDLDAMIEAGQLAERVVGHPLPGKLKSGGNLEKYRARARAAAPATA"
     gene            2751..4025
                     /locus_tag="VNU03_23450"
     CDS             2751..4025
                     /locus_tag="VNU03_23450"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HWP77499.1"
                     /translation="MKPRVVVISIDGFAAFYWDDPQARLPTLRRLAERGARAAGMETV
                     FPSTTWPTHVSMVTGVSPRAHGVVANHILNRRTGATEDLTGDPIYDAPALLRAPTVYD
                     LAHRAGHRTAAIDWPATRNATTLDFNLPFFKDQRVFETQTAKAVWDELTALGFPMHRQ
                     GEWALLPRRLHKDEMVGRVATHVAHRHEPALLLLHFLCADSLQHLHGPRSPEAYWAIE
                     YVDGLIGRFLATLGDPGLDRTSVFVVSDHGFLPSRREIRPNVRLRKLGAQREARFVMN
                     HGAGALYRLDADPSALAQLAREIASIEGVSGMWPAAEYGALGLPSPAAHHQVADVMFE
                     AAPGYSFGDGAGGGGADGDDEHGAPRYLGTHGQRPLYADNAAFFLAAGAGIRRDVTLG
                     SIRSRDVAPTVAATLGLRMEAVEGAVLARALD"
     gene            complement(4022..>5086)
                     /locus_tag="VNU03_23455"
     CDS             complement(4022..>5086)
                     /locus_tag="VNU03_23455"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77500.1"
                     /translation="LLPARLAFYGGQIVPRKYVEAVGNDSFNAKPVGTGPVRFGSWVK
                     DDRAVLEANPDYWGGRIDVDRWIMRPIPETAPRIAALLKGEVDIITQLPPDQAERVTG
                     NPSTRVTGALYAGLYVLGVNSKRPPLDNPLVKQALSLAIDREAIVKELWRGRGLVPNG
                     PIAKGDNHYDASLPPLVYRPGEARDLLKKAGYRNEEIFLETTVAYVSQDKAMSEAIAA
                     MWREIGVNVKVEVIEYSVRAQKGREKSFKGLWWSDPTSTLGDPDGMMWRLLGPGGPMD
                     YWRDARWDELGNAARFSVDERFRGQAYREMTKTFLEVLPWLPVIQPYEDYGLQKYVDW
                     TANPNQQFELRRFNFKLKRA"
BASE COUNT          731 a         1862 c         1727 g          766 t
ORIGIN      
        1 atttcggtgg ggcgtctgct ctgccgaatc ctgccgtttt ccttgagcga acacccccct
       61 ccctgggcgc ggcgaggccc gcggccggct gagctgtcgt gagatcacct gcccagactt
      121 acgcggtagt tccttccact ccctgcttac caggctcggg tcggtgcttc caccgcgcgt
      181 gcttgcattg aataacgcca ctggcatgcg ctatgcggca actccttgcg gggagttgct
      241 ctcgactcgt cgagtcaggg ggtcaatcat gaaaacccgg gtcctgttgt tcgtgatcgg
      301 cagcgcagcg ctcttcggat gcgccacacg acagctaccg gaggagccct tgaacgcccg
      361 ccttgcagag tacagaacgg cgggcgtttc ggcggacagc cgaggcggcg cgatcacggt
      421 aaaccctgag caggtgttcg ggaactagtc ggccctgacg aacggctggg tagctcccga
      481 ggacccggac caagcttcgc tgcgatcggc gctgctggcg ttcgagaact agctcctcgc
      541 gttgcagtac aatgcggggc gacatgacca gcgcgctacc gctcgagggc ctgcgtgtcc
      601 tcgagctggg ccacatcatc gcggggccct ccgcgggcct cctgctcgcc gacctgggcg
      661 ccgacgtcat caagatcgag cggcccggcg agggcgacca gtcgcgcggc atgccggccg
      721 ggacctcggc caactttcac ttcctcaacc gcaacaagcg ctccatcgcg atcgacctca
      781 agggctcgga ggaggcccgc gcgctcttcc tgcggctggt ggccggctcc gacgtggtca
      841 tcgacaactt cgcccacggc gcggtggagg ccctcggcct gggctacgat gtgctgaccc
      901 ggaccaatcc cggcatcatc tacctggcgc tcaagggctt cctcccgggc ccgcacgagg
      961 cgcgcccctt cctcgacgag ctggcccaga tgtccgccgg gctcgccttc atgaccgggc
     1021 cccgcggtca gccgctgcgc gcgggcgcct cgatcgtcga cgtgggcgcc gccgcctacg
     1081 gagtggtcgc ggtgctcgcc gcgctccatc agcgcgcgga gaccaagcgc ggccagaaga
     1141 tcacctccgg cctcttcgag accaccgtct tctgggtcgg ccagtggctc gcgcagtacg
     1201 cggccaccgg ccagccgtcg gtgccgatgc cggagatccg ccagggcacg cggatgggct
     1261 ggggcgtcta ccagctcttc accggcgccg acggggagca ggtcttcatc ggcatcacct
     1321 ccaacggcca ctgggagcgc ttctgcgccg agttcggcct cgacgatctc ggggccgatc
     1381 cgcgcctcga cgataacgcc aagcgcgtgg ccgcccgcga ctggctgccc ggccgcatcg
     1441 cccaggagat ggcgcgccac tcgagcgccg agctatcgga ccgcctcgag aaggcgcggg
     1501 tgccttacgc tccgctgcgc cggccggatc agctggtgga cgatccgcat ctcaacgcgt
     1561 ccgggcagtt catgatcacc cccctgcccg accgcccgcc ggccaagctg ccaaagctgc
     1621 cggtgcacag caccgcgttc gagatggagc tgcggcggcc cgccccgcgc ctgggcgagc
     1681 acacccgcga ggtgctggcc gagctgggcc tcgacaagga cgagatcgac gcgctcgcgt
     1741 cacggagggt catatgagcg acatgccaag gcacgtgcac atcaaggaag tgggtccgcg
     1801 cgagggcttc cagttcgaga agggcgacat caccctcgcg gccaagatct ccctcgtgga
     1861 cgcgctgtcc gacaccggcg tcgggacgat cgagttcacc tcgttcgtga gcccgaagtg
     1921 ggtgccccag atggccgacg ccgagcagat ggtggccggc atcaagcggg tgcccggggt
     1981 ggcctacgag gcgatctggc tcaacgaggc cggcctcgag cgggccgcgc gcctgcgcga
     2041 gtcgctcgcg ctgcgcccga tgttcggggt ggcggccacc gacaccttca cgcgcaagaa
     2101 caccaaccgc agcgtggacg agaaggtcgc cgagctgccg ggctgggccc ggcgctacca
     2161 ggccctcggc atggactgga tcgacgcggg cgtcatggcc gccttcggct gcaactacga
     2221 gggcgacgtg acgccggccc aggtgatcgg cgtcatcgag aaggtggagg cgcaggcggt
     2281 gggctgcggc tcgcggctcg gcaacatcag cctggccgac accatgggct gggccaaccc
     2341 gctccagatc aagcggctgg tgggcgcggt gcgggcgcgg tggcccgaga tcccggtgaa
     2401 gctccatctg cacgacaccc gcgcgctcgc catcgccaac gtggtggccg cgctcgagat
     2461 gggcgtggat cgcttcgaca gcgcggtcgc gggcctgggc ggctgcccct tcgcggccca
     2521 caagggcgcg gcgggcaacg tgaccaccga ggacctggtc ttcctctgcc aggagctggg
     2581 ggtggacacc ggcatcgacc tcgacgccat gatcgaggcc ggccagctcg ccgagcgcgt
     2641 cgtgggccac ccgctgcccg gcaagctgaa gtcgggcggc aacctcgaga agtaccgcgc
     2701 ccgcgcgcgg gccgccgccc ccgcgacggc ctgagtcgcc ggtccgcctc gtgaagcccc
     2761 gcgtggtcgt catcagcatc gacggcttcg cggcgttcta ctgggacgac ccgcaggcgc
     2821 gcctgcccac gctccgacgc ctcgccgagc gcggcgcccg cgccgcggga atggagacgg
     2881 tctttcccag caccacgtgg cctacccacg tctccatggt gaccggcgtc agcccgcgcg
     2941 cgcacggcgt cgtggccaac cacatcctga accgccggac cggcgccacc gaggacctga
     3001 ccggcgatcc catctacgac gcgcccgcgc tgctgcgcgc gcccaccgtg tacgacctgg
     3061 ctcatcgggc cgggcatcgc accgcggcca tcgactggcc ggccacccgg aacgcgacca
     3121 ccctcgattt caacctcccc ttcttcaagg atcagcgggt gttcgagacc cagaccgcga
     3181 aggcggtgtg ggacgagctg accgcgctcg gcttcccgat gcaccgccag ggggagtggg
     3241 cgctcctgcc ccggcgcctg cacaaggacg agatggtggg ccgggtggcc acccacgtgg
     3301 cccaccgcca cgagcccgcc ctgctcctgc tccacttcct ctgcgcggac agcctccagc
     3361 acctccacgg cccgcgctcg ccggaggcct actgggcgat cgagtacgtc gacggcctga
     3421 tcggccgctt cctcgccacc ctcggcgatc ccggcctcga ccgcacgtcg gtgttcgtgg
     3481 tatccgacca cggcttcctg ccctcgcgcc gcgagatccg gcccaacgtc cggttgcgga
     3541 agctcggcgc gcagcgcgag gcccgcttcg tgatgaatca cggcgcgggc gccctctatc
     3601 gtctcgacgc cgatccctcg gccctcgcgc agctcgcgcg cgagatcgcg tccatcgagg
     3661 gcgtgagcgg catgtggccc gcggcggagt acggggccct cggcctgccc tcgcccgccg
     3721 cgcaccacca agtcgccgac gtgatgttcg aggccgcgcc cggctattcc tttggtgatg
     3781 gtgcgggcgg tggcggcgcg gacggagacg acgagcacgg cgcgcccagg tacctcggca
     3841 cccacggcca gcgcccgctc tacgccgaca acgcggcctt cttcctggcc gccggcgcgg
     3901 ggatccgccg cgacgtcacc ctcggctcga tccggagccg agatgtcgcc ccgacggtgg
     3961 cggccaccct cggcctccgc atggaggcgg tcgagggcgc cgtcctcgcc cgggccctcg
     4021 actaggcgcg cttcagcttg aagttgaagc gccggagctc gaactgctgg ttcgggttcg
     4081 cggtccagtc cacgtacttc tgcagaccgt agtcctcgta gggctggatg accggcagcc
     4141 acgggagcac ctcgaggaag gtcttggtca tctcgcgata ggcctggccg cggaagcgct
     4201 cgtcgacgct gaagcgcgcc gcgttgccca gctcgtccca gcgcgcgtcg cgccagtagt
     4261 ccatcggccc gccggggccg agcaggcgcc acatcatgcc gtcggggtcg cccagggtgg
     4321 aggtgggatc ggaccaccac aggcccttga agctcttctc gcggcccttc tgcgcgcgga
     4381 ccgaatactc gatgacctcg accttcacgt tgaccccgat ctcgcgccac atcgcggcga
     4441 tggcttccga catcgccttg tcctgcgaga cgtaggccac cgtggtctcg aggaagatct
     4501 cctcgttccg gtagcccgcc ttcttcagca gatcgcgcgc ctcgcccggc cggtagacca
     4561 gcggcggcag gctcgcgtcg tagtggttgt cgcccttcgc gatggggccg ttgggcacga
     4621 gcccgcggcc gcgccacagc tccttcacga tcgcctcgcg gtcgatcgcg agcgacaggg
     4681 cctgcttcac gagcggattg tcgagcggcg ggcgcttgct gttcacgccc agcacgtaga
     4741 ggccggcgta gagcgcgccg gtgacccggg tcgaggggtt gccggtcacc cgctccgcct
     4801 ggtcgggtgg gagctgggtg atgatgtcga cctcaccctt gagcagcgcg gcgatgcgag
     4861 gcgcggtctc cggaatcgga cgcatgatcc agcggtccac gtcgatccgg ccaccccagt
     4921 agtcggggtt ggcctcgagc accgcccggt cgtccttcac ccacgagccg aagcgcaccg
     4981 gcccggtgcc taccggcttg gcgttgaagg agtcgttgcc caccgcctcg acgtacttcc
     5041 tcggcacgat ctgccccccg tagaaggcga gccgcgcggg caggag
//
LOCUS       DATMCV010001023         9444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_53613, whole genome shotgun sequence.
ACCESSION   DATMCV010001023 DATMCV010000000
VERSION     DATMCV010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53613"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VNU03_23460"
     CDS             complement(<1..408)
                     /locus_tag="VNU03_23460"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP77501.1"
                     /translation="MTGGAVRVGLEIARTLADAGADVAIGYLRSAAAARAAVRELEAR
                     GVRAVALRADVGRPREARALVADAVKRLGRLDVLVNNAALFFRTPVETTTPAQFDRLL
                     AVNLRAPFFCSQAAARAMGRRGGRIINIADVGAV"
     gene            472..1083
                     /locus_tag="VNU03_23465"
     CDS             472..1083
                     /locus_tag="VNU03_23465"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HWP77502.1"
                     /translation="MSGSFRNWVKTRFITGLFSTVPAIATAWLLWVFWNAIDDFFSPV
                     YTQILGRRIPGLGFLTAVVIIFIMGTIARNVFGRRVLARIEMLFDHVPIFRSIYPSVK
                     QLLESFSPQKLNSFKSVVLAEHPRKGEYVFGFVTSEVLVEGLEGKRAMVTVFVPTNNL
                     YLGDVIVLPKEEMLPTGLTVEEGIRIILSAGTATPARLPRERL"
     gene            complement(1084..1707)
                     /locus_tag="VNU03_23470"
     CDS             complement(1084..1707)
                     /locus_tag="VNU03_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWP77503.1"
                     /translation="MEEPVVVPVPGGPSLEGRLALVPDPKGGFVLCHPHPLYGGDMDN
                     PVIMRAAEVVREVGHATLRFNFRGVGASTGTHDKGAGEQDDVRAAVAALAGHLPPGSS
                     VGVIGYSFGAWTAARAMRPGAAPLGLIAPPLGMFDFQLPTSPAGRMLVVAGTRDTYCP
                     VDALHELARTTRAEERIIEGADHFFFGKLYPLGEAIRAWVTGAPPAR"
     gene            1788..2306
                     /locus_tag="VNU03_23475"
     CDS             1788..2306
                     /locus_tag="VNU03_23475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77504.1"
                     /translation="MPTLGCLWSRLRLERLVDGALGPRMGRVAASHASRCGDCRAEVD
                     RLRRLRALVQSAEVSVTDPDWAAFWPAVRQRIAAEHPAPVRDAWWLPFWKPVWGHPRV
                     ALGGLVVSSLAAALVLWPSPETTKAAMAAPVQVQEVSTADPDRSVMVYSNPDDDVTVI
                     WVFNPSEQDEQS"
     gene            complement(2350..3189)
                     /locus_tag="VNU03_23480"
     CDS             complement(2350..3189)
                     /locus_tag="VNU03_23480"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP77505.1"
                     /translation="MLWALLPAIVIVAVGCADVSGQALQQDVAQLRQDLNSLTLAVHR
                     GRGDTDTVVGQLDRRTREQNAESAKQTAALGARVDALSAEVARMTARLDELSQRMDAL
                     GRSAPSGGGSGVPPPARSGGTPIPAPSGSLATAPRASGGAGTAEESYQAAYLDFSKGL
                     YPLSITEFRDFLRRFPDSALADSAQYWIGEAYFSMARATASQPDKARENLEQAVQEFR
                     KVMVTYPRGSKVPTALYKEALALVELKQTALAQARLQYLVEHFPQSEEAPLAKERLAS
                     LKD"
     gene            complement(3252..3824)
                     /gene="pal"
                     /locus_tag="VNU03_23485"
     CDS             complement(3252..3824)
                     /gene="pal"
                     /locus_tag="VNU03_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HWP77506.1"
                     /translation="MAQRRGSALLLAVPVIALSLFLVGCPKRPATTAASAPAPTGSPS
                     AAAPSTTPSPSMTPSPVAPSTASPTSPPAPSEFRATDNLKDVFFDFDKYDIRPTDAKM
                     LDTNAAWLKSNDNLVLIEGHCDERGTNEYNLALGERRAKAAMNYLVAQGVQASRITII
                     SYGKERPTCTEHSESCWAQNRRAHFLVKAR"
     gene            complement(3894..5228)
                     /gene="tolB"
                     /locus_tag="VNU03_23490"
     CDS             complement(3894..5228)
                     /gene="tolB"
                     /locus_tag="VNU03_23490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HWP77507.1"
                     /translation="MRRPGRPLVWLVASVVVLVLAGVLASPPPVRSQSDIFVNVLATG
                     AKKLNIVIPNFAVVAGTDTAGLGRSVPQVTARDLTFSALFSVVSDVPPIPTADPAAVR
                     ASFANFAAAGAHAGLQGLLSLRGDRTEVEMRLYDLTSPEFRLIATKKIELPANQPRRL
                     SHKIADEVVMQFTGEAGIADTKIAYVNTSAGSKEVSLMDYDGAGPAHVTSNKSINLSP
                     MWSPDTRSLAFTSYMRGYPYLYRMFPFESRPVQVVAGFLGINSSPAFSPDGKFLAMTL
                     SRDGNPEVYVLNLATGAFRRLTSYSGIDTEPTWSPTGREIAFVSERAGGAHVFVMDAE
                     GANVRRLTQAGFNTQPRWSPKGDTIVFTSRQGNFDLWAVSPDGSNLRRLTAGPGNNEG
                     ASWAPNGRHLVFQSSRSGGYQLFTMLADGSEQQAIPRTPGESTSAAWSPRLP"
     gene            complement(5225..6106)
                     /locus_tag="VNU03_23495"
     CDS             complement(5225..6106)
                     /locus_tag="VNU03_23495"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWP77508.1"
                     /translation="MSSTMAARRYSMGPLPDPGLRRPGPALRRSRLPLTAIGFSAIAH
                     VVVFGGMAVFVMWTGWRPEKVQVVNLVPMVAAVGTPNAPPAPALPTRPQAPVAPRVPD
                     PEPEPKATTAPREAPRREAPSLPAPSLPAAKPLPPRSTALPRPGEKELPPLATPPSGS
                     PKGEKTAEAPRAPETAALGAPTGSAAGQGALTLDVSDFPYAWWVRAAIAKVQDQWQRQ
                     NQFSEPPQKPQLLVEVQRDGSIARIPMIEKSSGNTVYDQAAVRAVMAASPLPKLPDEW
                     TGGAVLRLHISFDLARR"
     gene            complement(6103..6540)
                     /locus_tag="VNU03_23500"
     CDS             complement(6103..6540)
                     /locus_tag="VNU03_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWP77509.1"
                     /translation="MAIKVDGSDNGGGGYRIGGTLAEINIIPLVDVTLVLLLIFMLTA
                     PLMYRGIDVNLPKTAGKPTATEERMVLTLSKEQTIYVNDKPVPLGSLEQTLRDLFKNR
                     QDKTLYLRADQALQYGFVVETMDRIRRSGIEKLGMVTEPTRAR"
     gene            complement(6530..7270)
                     /locus_tag="VNU03_23505"
     CDS             complement(6530..7270)
                     /locus_tag="VNU03_23505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWP77510.1"
                     /translation="MPGSGSVNGGVAELVFSAGPVAKLILLLLAVFSVICWALIVEKW
                     WEFRKIRRESLRFRQIFREARRFSVVYAAAKKHRDSPLAQLYVSAGQELVTAYGNAEI
                     VDHVLDDADEGLPAERLEASSRALRRAGSSEVARMERYLPFLATVASSAPFIGLFGTV
                     WGIMTAFQGIGQQGSANLAVVAPGISEALIATAAGLGAAIPAVMGYNFFVNRTKHWAM
                     EMEGFTLDLLNAFARPVHKLARMAKDGD"
     gene            7432..8217
                     /gene="lgt"
                     /locus_tag="VNU03_23510"
     CDS             7432..8217
                     /gene="lgt"
                     /locus_tag="VNU03_23510"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWP77511.1"
                     /translation="MFHSPGAIALHVGPIAIRWYGVLMATAMALGLWLGHRDARRRGI
                     DPESLLKAAELALLGGLVGARLYYVLFNLDYYGQFPGKIFALWEGGLAIHGGVIGGIL
                     VGGGYAWRRGLPVRQYLDIAAPSLALGQAIGRWGNFFNEEAFGRPTDLPWKLFISPVH
                     RPLQYAQSEYFHPTFLYESLWDLIVFAVLLLGLRDRLERAPGALFLAYLGLYSAGRLF
                     TEGLRTDALMLGPLRIAQVVSLLGMAVAAVGVPLLLRHQSRAA"
     gene            8229..8912
                     /locus_tag="VNU03_23515"
     CDS             8229..8912
                     /locus_tag="VNU03_23515"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HWP77512.1"
                     /translation="MKRYASRDGRLRLRVGDSRRLPEIPTGSVGCVLTSPPYWVRGRG
                     RARAERYARELATGFGPEWRRVLADHGDLWLVIGDRHDGLEWIGIDGLVTRWLGRVGF
                     RLQAKGLWAQVRSRERWDNRVNYLLRFRKAGHPVRPGGATLCWMLPLPRSHPDSLWDA
                     TPDPVLRALLEISARRGAVLDPFAGAGTVGRIAVELGREWIGVERDPHMAAVAARRLR
                     LRPVTPSPR"
     gene            complement(8893..>9444)
                     /locus_tag="VNU03_23520"
     CDS             complement(8893..>9444)
                     /locus_tag="VNU03_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011044878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWP77513.1"
                     /translation="RKPNTVYSEMALAEFFQTDAAINPGNSGGPMFNMAGEVIGIASH
                     NITKSGGSEGLGFVVTINMARRLLLEQRSFWSGMSGVIVGGQVARALNLPQAVGLMVQ
                     QVAANSPAAQMDLRPGVIPASIEGKSFLLGGDIILAVQEREIGTETYEALQARLRSLP
                     RGSTISVLVLRDGRPVTLNAVKE"
BASE COUNT         1293 a         3246 c         3427 g         1478 t
ORIGIN      
        1 caccgcgccc acgtcggcga tgttgatgat gcgcccgccg cggcgcccca tcgcccgcgc
       61 ggcggcctgg ctgcagaaga acggggcgcg caggttcacc gcgaggaggc ggtcgaactg
      121 ggcgggggtg gtggtctcga ccggggtgcg gaagaagagc gccgcgttgt tgacgaggac
      181 gtcgaggcgc ccgagccgct tcaccgcgtc ggcgacgagg gcccgcgcct cgcgggggcg
      241 gcccacgtcg gcgcgcaggg ccaccgcgcg caccccgcgc gcctccagct cgcgcaccgc
      301 cgcgcgcgcg gccgccgccg agcgcagata gccgatcgcc acgtccgcgc ccgcgtcggc
      361 gagcgtgcgc gcgatctcga gaccgacgcg caccgcgccg ccggtcacca gcgcgacccg
      421 gccggtcagt tccttgactt gtccggagcc gtgtgttgtc atcgatccaa catgtcgggt
      481 agtttccgca actgggtgaa gactcgcttc atcaccgggc tgttctccac cgtccccgcg
      541 atcgcgaccg cttggctgct gtgggtgttc tggaacgcca tcgacgattt cttctccccg
      601 gtgtacaccc agattctcgg gcggcgcatc cccggcctgg gcttcctgac cgcggtcgtg
      661 atcatcttca tcatgggcac catcgcccgc aacgtgttcg ggcggcgggt gctggcccgc
      721 atcgagatgc tcttcgacca cgtgccgatc ttccggagca tctacccatc cgtgaagcag
      781 ctgctggagt ccttctcgcc ccagaagctc aactccttca agtcggtggt gctcgccgag
      841 catccccgca agggggagta cgtgttcggc ttcgtcacct ccgaggtcct ggtcgagggg
      901 ctcgagggca agcgggccat ggtcaccgtg ttcgtgccca ccaacaacct gtacctgggc
      961 gacgtgatcg tgctgcccaa ggaagagatg ctgcccaccg gcctcaccgt cgaggagggc
     1021 atccgcatca tcctctcggc cgggaccgcc accccggccc gcctgccgcg cgagcggctc
     1081 tgatcaccgg gcgggcggcg ctcccgtcac ccacgcccgg atggcctccc cgagcgggta
     1141 gagcttgccg aagaagaagt ggtccgcgcc ctcgatgatc cgctcctccg cccgggtggt
     1201 ccgggcgagc tcgtggaggg cgtcgaccgg gcagtaggtg tcgcgcgttc ccgccaccac
     1261 gagcatgcgg ccggccgggg aggtcgggag ctggaagtcg aacattccga gcgggggcgc
     1321 gatgaggccg agcggcgcgg cccccggccg catggctcgg gcggcggtcc aggccccgaa
     1381 ggaatagccg attaccccga ccgaactgcc gggggggaga tgcccggcca gggccgccac
     1441 ggcggcccgt acgtcgtcct gctccccggc gcccttgtcg tgggtcccgg tcgaggcgcc
     1501 caccccccgg aaattgaagc gcagagtggc gtgcccgacc tcgcggacga cctctgcggc
     1561 ccgcatgatc accggattgt ccatgtcccc gccgtagagt gggtgggggt ggcacaggac
     1621 gaagcccccc ttcgggtcgg ggaccagggc cagccggcct tccaggctcg ggccgccggg
     1681 caccgggacg acgacgggct cttccatggc gctccgacct tagggggtcg ccggggaata
     1741 ttcaaggggc agcccgcgtc tgaacctgcg aggttataat ttctctcatg ccgacgctgg
     1801 gatgcctgtg gagccgcctt cgactggagc gcctggtcga cggggccctc gggccacgga
     1861 tggggcgcgt ggccgcctct cacgcttccc gctgtgggga ctgccgggcc gaggtggatc
     1921 gcctccgtcg cctgcgggcc ctggtgcaga gcgccgaggt gtccgtgacc gatcccgact
     1981 gggcggcttt ctggcccgcc gtccgtcagc gcatcgccgc ggagcatccg gcgcccgtcc
     2041 gggacgcgtg gtggctcccc ttctggaagc cggtgtgggg gcatccgcgc gtggccctgg
     2101 ggggcctcgt ggtgtcgagc ctggcggcgg ccctcgtgct ctggccgagc cccgagacca
     2161 ccaaggcggc gatggcggcc ccggtgcagg tgcaggaggt gtcgaccgcc gatccggatc
     2221 gcagcgtgat ggtgtactcg aatccggacg acgacgtcac cgtgatctgg gtcttcaacc
     2281 cgtccgagca ggacgagcag tcctgagccg gcgccgcgcc gggcggcgcg gcggcccttc
     2341 cacgaccggc taatccttca agctggccag ccgctccttc gccagcgggg cctcttccga
     2401 ctgcgggaag tgctccacca ggtactgcag gcgcgcctgg gcgagcgcgg tctgcttgag
     2461 ctcgacgagc gcgagcgctt ccttgtagag cgcggtcggc accttgctgc cccgcgggta
     2521 ggtcaccatc accttgcgaa actcctgcac cgcctgctcg aggttctcgc gggccttgtc
     2581 cggctggctc gcggtggcgc gcgccatgct gaagtaggcc tcgccgatcc agtactgggc
     2641 gctgtccgcg agcgcggagt ccgggaagcg gcggaggaaa tcgcggaact cggtgatcga
     2701 cagcgggtag agccccttgc tgaaatcgag atacgcggcc tggtagctct cctccgcggt
     2761 gccggcgccg ccggaggccc ggggggccgt cgcgagcgag cccgacggag cgggaatggg
     2821 cgtgccgccg gagcgggcag ggggcggaac gccggagccg ccgccgctcg gggcggagcg
     2881 cccgagcgcg tccatgcgct gggagagctc gtcgagccgg gcggtcatcc gcgcgacctc
     2941 ggcggagagc gcgtccacgc gcgcgccgag ggccgcggtc tgcttggcgc tctccgcgtt
     3001 ctgctcgcgg gtgcgtcggt cgagctggcc caccacggtg tcggtgtccc cccggccgcg
     3061 gtgcaccgcg agggtcagcg agttcagatc ctggcggagc tgcgcgacgt cctgctgcag
     3121 cgcctgcccg gagacgtcgg cgcagcccac cgcgacgatc acgatcgcgg gcaggagggc
     3181 ccaaagcacg aaggggagcc gatggctccc cttcgtgatc tcacggcgca cgggatgcgg
     3241 ccccgtgcgg atcagcgggc cttcaccagg aagtgcgccc gacggttctg ggcccagcag
     3301 gactcggagt gctcggtgca cgtcggccgc tccttgccgt aggagatgat cgtgatccgg
     3361 ctcgcctgca cgccctgggc gacgagatag ttcatggccg ccttcgcgcg ccgctcgccg
     3421 agggccaggt tgtactcgtt ggtgccgcgc tcgtcgcagt ggccctcgat caggaccagg
     3481 ttgtcgttcg acttcagcca ggccgcgttg gtgtcgagca tcttcgcgtc ggtcggccgg
     3541 atgtcgtact tgtcgaagtc gaagaacacg tccttgaggt tgtcggtggc ccggaactcg
     3601 ctcggcgcgg gcggcgaggt cgggctcgcg gtcgacggcg ccaccggtga cggcgtcatc
     3661 gacggcgacg gcgtcgtcga tggggcggcg gcggagggcg agccggtcgg agcaggcgcg
     3721 gaagccgcgg tcgtggcagg tcgcttggga cagcccacca ggaagagaga gagggcgatc
     3781 accggcacgg cgagcagcag cgcgcttccc cgtcgttgcg ccattagtcc aatcctcctt
     3841 gagttcgtcg aattcgtcat atcaccgcga gtttgcgtta ctgaagcatc ctatcacgga
     3901 aggcgcggcg accaagcagc acttgtcgat tcgcccgggg tccgggggat cgcctgttgc
     3961 tcggatccgt ccgcgagcat ggtgaacagc tggtatccgc cggaccggct cgactggaag
     4021 acgaggtgcc ggccgttggg cgcccaggat gccccctcgt tgttgccggg tcccgcggtc
     4081 aggcggcgca ggttggagcc gtcggggctg accgcccaca ggtcaaaatt tccctgtcgc
     4141 gaggtgaaca cgatcgtgtc gcccttgggc gaccagcgtg gctgggtgtt gaagccggcc
     4201 tgggtgagac ggcgcacgtt ggcgccctcc gcgtccatca cgaacacgtg ggccccgccc
     4261 gcccgctccg acacgaacgc gatctcccgg ccggtgggcg accaggtggg ctcggtgtcg
     4321 atgccgctgt agctggtgag ccgccggaac gccccggtgg cgaggttcag gacgtacacc
     4381 tccgggttgc cgtcccgcga gagggtcatg gcgaggaact tcccgtcggg gctgaacgcg
     4441 ggcgagctgt tgatgccgag gaatccggcc accacctgca ccggccggct ctcgaacggg
     4501 aacatccggt aaaggtaggg atacccgcgc atgtacgacg tgaaggcgag ggaccgcgtg
     4561 tccgggctcc acatcggcga caggttgatc gacttgttcg acgtcacgtg ggcgggcccc
     4621 gcgccgtcgt agtccatcaa gctgacctcc ttggagcccg cgctcgtgtt cacgtaggcg
     4681 atcttggtgt ccgcgatgcc ggcctcgccg gtgaactgca tgaccacctc gtccgcgatc
     4741 ttgtgggaca ggcgccgggg ctggttggcg ggcagctcga tcttcttggt cgcgatcagc
     4801 cggaactccg gcgaggtgag atcgtagagc cgcatctcga cctcggtccg gtcgccgcgc
     4861 aggctgagca gcccctgcag ccccgcgtgg gcccccgcgg ccgcgaagtt ggcgaagctg
     4921 gcgcggaccg cggcgggatc ggcggtcggg atcggcggca cgtccgagac cacgctgaag
     4981 agcgcggaga acgtgagatc ccgggccgtg acctgcggga cgctccggcc gaggccggcg
     5041 gtgtcggtgc cggccaccac cgcgaagttc gggatcacga tgttgagctt cttggccccg
     5101 gtggccagca cgttcacgaa gatgtcggat tgcgagcgca ccgggggcgg cgaggcgagc
     5161 acgccggcga ggacgagcac cacgacggaa gcgaccagcc agacgagcgg ccgacccggg
     5221 cgcctcatcg gcgcgccagg tcgaagctga tgtgaaggcg cagcacggct cccccggtcc
     5281 actcgtccgg cagcttcggg agcgggctcg ccgccatgac cgcgcgcacc gcggcctggt
     5341 catacacggt attgccggag ctcttctcga tcattgggat cctcgctatc gagccatcac
     5401 gctgaacctc caccaggagc tggggcttct ggggcggctc gctgaattgg ttttgtcgtt
     5461 gccactgatc ctggaccttg gcgatcgcgg cgcggaccca ccacgcgtag ggaaagtccg
     5521 agacgtcgag ggtcagcgcg ccctggccgg cggccgagcc ggtcggggcg ccgagcgcgg
     5581 cggtctccgg cgctcggggc gcctccgcgg tcttctcgcc cttcgggctc cccgagggcg
     5641 gtgtggccag aggcggcagc tccttctcgc cgggccgcgg cagcgcggtg ctgcgcggcg
     5701 ggagcggctt ggccgcgggg agcgacggcg cgggcaggct cggggcctcg cggcgcggcg
     5761 cctcgcgggg tgcggtggtg gctttgggct cgggctccgg gtcgggcacc cgcggggcca
     5821 ccggcgcctg cgggcgggtc ggcagggccg gggccggggg ggcgttgggc gtgccgaccg
     5881 cggcgaccat cgggaccagg ttgacgacct ggaccttctc cggacgccag ccggtccaca
     5941 tcacgaagac ggccatcccg ccgaagacca ccacgtgggc gatcgcggag aacccgatcg
     6001 cggtcagggg taggcgcgac cgtcgaaggg ccggtcccgg ccgcctcaga ccggggtcgg
     6061 ggagcgggcc catggaatag cgccgggccg ccatcgtgga gctcatcgag cgcgggtcgg
     6121 ctccgtcacc atgccgagct tctcgatgcc cgagcggcga atccggtcca tggtctcgac
     6181 cacgaagccg tactgcaggg cctggtccgc ccgcaggtag agggtcttgt cctggcggtt
     6241 cttgaagaga tcgcgcagcg tctgctcgag ggagccgagc ggcaccggct tgtcgttgac
     6301 gtagatggtc tgctccttgg agagggtcag gaccatccgc tcctcggtcg cggtgggctt
     6361 gcccgccgtc ttgggcaggt tcacgtcgat gccgcggtac atcagcgggg cggtgagcat
     6421 gaagatcagc agcaacacca gcgtcacgtc gacgagcggg atgatgttga tctcggcgag
     6481 ggtgcccccg atgcggtagc cgcctccgcc gttgtcggag ccgtcgacct taatcgccat
     6541 ctttcgccat tcgcgccagc ttgtgcaccg ggcgcgcgaa cgcgttcagc aggtcgagcg
     6601 tgaagccctc catctccatc gcccagtgct tcgtccggtt cacgaagaag ttgtagccca
     6661 tgaccgcggg gatcgcggcg ccgaggcccg ccgcggtggc gatcagggcc tccgagatgc
     6721 cgggcgccac caccgcgagg ttggccgagc cctgctgccc gatgccctgg aacgcggtca
     6781 tgatgcccca caccgtgcca aagagaccga tgaacggcgc cgagctggcc accgtggcca
     6841 ggaacggcag gtagcgctcc atgcgcgcca cctccgacga ccccgcgcgg cggagcgccc
     6901 ggctcgacgc ctcgagccgc tccgcgggca ggccctcgtc cgcgtcgtcc agcacgtggt
     6961 ccacgatctc cgcgttgccg tacgcggtga ccagctcctg gcccgccgac acgtagagct
     7021 gggcgagcgg gctgtcgcgg tgcttcttgg ccgccgcgta gaccaccgag aagcggcgcg
     7081 cctcgcggaa gatctggcgg aagcgcagcg actcgcggcg gatcttccgg aactcccacc
     7141 acttctcgac gataagcgcc cagcagatga ccgagaagac ggcgagaagg agcaggatta
     7201 acttggcgac ggggccggcg ctgaagacga gctctgcaac gccgccgttg acgcttcctg
     7261 accctggcac tcggagctcc ttatcgggtg gatggtgaac tggcgaccag cggaattgct
     7321 tcatttgatg ataccggacg atttttgaga gtgtcaacaa attcgccggc aaaacgcctg
     7381 aaaagtcgct gaattattga cagtcccgac ccgccgccgt ataagccttc tatgttccac
     7441 tcgcccggtg cgatcgcgct gcacgtgggg ccgatcgcga tccggtggta cggcgtgctc
     7501 atggccaccg cgatggccct cggtctctgg ctcggacacc gggatgcgcg ccgccgcggc
     7561 atcgatcccg agagcctgct caaggccgcg gagctggccc tgcttggcgg cctcgtcggc
     7621 gcacgtctct actacgtgct gttcaacctc gactactacg gccagttccc cggtaaaatc
     7681 ttcgccctct gggagggcgg gctcgccatt cacggcggcg tgatcggcgg catcctcgtc
     7741 gggggcggct acgcctggcg acggggactg ccggtgcggc aatacctgga catcgcggcg
     7801 ccgagcctgg ccctcgggca ggccatcggg cggtggggca acttcttcaa cgaggaagcg
     7861 ttcggccgtc cgaccgatct gccgtggaag ctgttcatct ccccggtgca ccgcccgctc
     7921 cagtacgcgc agtcggagta cttccacccc acgttcctct acgagtcgct gtgggacctg
     7981 atcgtgttcg cggtcctgct cctgggcctg cgggatcggc tcgagcgggc gccgggggcg
     8041 ctcttcctgg cctacctcgg gctctactcg gcggggcggc tcttcaccga ggggctccgc
     8101 accgacgcgc tgatgctggg cccgctccgc atcgcccagg tcgtgagcct gctcggcatg
     8161 gcggtggctg cggtgggcgt gccgctgctc ctgcgtcacc agagccgcgc cgcctgagcc
     8221 ggccccgcgt gaagcgctac gcgagccgcg acggccggct ccgtctgcgg gtcggcgaca
     8281 gccgccgtct ccccgagatc ccgaccggca gtgtcggctg cgtgctcacc tcgccgccct
     8341 actgggtgcg ggggcgggga cgcgcgcgcg ccgagcggta cgcgcgcgag ctcgccaccg
     8401 gcttcggccc cgagtggcgc cgggtcctgg ccgaccacgg cgacctgtgg ctcgtcatcg
     8461 gcgaccgcca cgacggcctc gagtggatcg gcatcgacgg cctcgtgacg agatggctcg
     8521 ggcgtgtggg gttccgtctc caggccaagg ggctctgggc ccaggtgcgc tcccgggagc
     8581 gctgggacaa ccgggtgaac tacctgctgc ggttccggaa ggcggggcac ccggtccgtc
     8641 cgggcggcgc caccctctgc tggatgctgc cgctgccgcg ctcgcatccg gacagcctgt
     8701 gggacgccac cccggacccg gtgctgcgcg ccctgctgga gatctccgcg cggcgcgggg
     8761 ccgtgctcga cccgttcgcg ggggcgggca cggtcgggcg catcgcggtc gagctcggtc
     8821 gcgagtggat cggggtggag cgggatccgc acatggccgc ggtggccgcg cgacggctcc
     8881 ggctccgccc ggtcactcct tcaccgcgtt gagggtcacc ggccgcccat cgcgcaggac
     8941 gagcaccgag atcgtgctcc cgcgcgggag gctccgcagc cgcgcctgca gtgcctcgta
     9001 ggtctcggtg ccgatctccc gctcctgcac ggccaggatg atgtcgccgc ccagcaggaa
     9061 cgacttgccc tcgatcgagg cggggatgac cccgggccgg agatccattt gggccgcggg
     9121 ggagttcgcc gcgacctgct gcaccatgag cccgaccgcc tggggcaggt tgagcgcgcg
     9181 ggcgacctgc ccgccgacga tcaccccgct catgccgctc cagaacgagc gctgctcgag
     9241 caggaggcgt cgggccatgt tgatggtgac cacgaagccc aggccctcgg acccgcccga
     9301 cttggtgatg ttgtggctcg cgatcccgat gacctcgccc gccatgttga acatgggccc
     9361 gcccgagttg ccgggattga tggcggcgtc ggtctggaag aactcggcga gcgccatctc
     9421 gctgtagacg gtgttgggct tgcg
//
LOCUS       DATMCV010001024         4121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_256369, whole genome shotgun sequence.
ACCESSION   DATMCV010001024 DATMCV010000000
VERSION     DATMCV010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256369"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2087)
                     /locus_tag="VNU03_23525"
     CDS             complement(<1..2087)
                     /locus_tag="VNU03_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP77514.1"
                     /translation="MQDLTPNEPLHPVARTLAAPASGLERFPPAARWDDWEELDSKAW
                     PRKVKRRFQLIPTICFNCEAACGLLAYVDVQSLKVHKFEGNPVHPGSRGRNCAKGPAT
                     LNQITDPERILYPLRRAAGRGEGKWERVTWDAVLDDIGGRIRKALVEGRQKEVMYHLG
                     RPGHELIYLQRVFHAWGIDGHNSHTNVCSASARAGYAFWHGLDRPSPDHSKARFILLL
                     SSHLEAGHYFNPHAQRIIDGKMAGAKICVVDTRLSNTASMADYWLSPWPGSEAAMLLA
                     IVHCMLRDGTWDREFVRRWVNWEEYLREERPDLPVTMAAFEEALKTLYASYTPEFAER
                     ESGVPAARIEEVAREVGRAGSALSTHVWRNAAAGNLYGWEVARALELIVVLAGAVATP
                     GGTAPSGWNKAVPAPPMMPPPGKVWSELLMPREYPLSFFEMSFLLPHFLKEARGKLAM
                     YFTRVYNPVWTNPDGMSWIEMLTDEAKVERHACLTPTWSETAWFADYVLPMGHASERH
                     DLMSQETHAARWIGFRQPVLRVALEKQGKTFDHTWQAHEAAGLGQVWEEDEFWIELSW
                     RIDPDGSLGIRKYFESPYRPGEKLRIEEFYRWIFEHSVPGLPAAAEREGLTPLAYMRK
                     YGAFLIAENVYGTHETPLTPAERDGAAPDAASHLVMKGGASVGVEVDGQWMGGFPTPS
                     RVLRPLPVLEGLG"
     gene            complement(2102..3673)
                     /locus_tag="VNU03_23530"
     CDS             complement(2102..3673)
                     /locus_tag="VNU03_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWP77515.1"
                     /translation="MRYGFVIDQRKCIGCHACTVACKEENQVPLGVNRTWVKYIEKGV
                     FPDTRRYFSVMRCNHCDNAPCVTICPTVALYRRPDGIVDFDGDRCIGCKSCMQACPYD
                     ALYIDPETKTAAKCHYCAHRVESGLEPACVVVCPVQAIVPGDLDDPHSTISRLVASQQ
                     TQVRKPEQGTRPKLYYLGAEAAALTPDMQERRGGFVFSQVGSIPSPGQPNRPPAPATA
                     RASSSATAATHGMDLLALARTVYDVAHPARPWGWKVAAYLWTKSIAAGAFLVASLGLA
                     AGLLTADALVALAVPVIGLAFLLLTSLLLVFDLKRPERFLYLVFKPNPRSWLVLGGVI
                     LFAAGLLGALWLLAGVMSNLLALQLLAWPTAAAAAATAGYSAFLFGQAEGRDFWQSPL
                     LLPHLLVAALVAGSASLLLAANALGSASIITGGLGLVLWGSLLVSALVLFAELFTAHG
                     TQDAARAARLLTHGHLRVALWGGVVVGGIVLPLVLLLSASVPFGSELAAGLALAGLWL
                     WEELWIRAGQSIPLS"
     gene            complement(3675..>4121)
                     /locus_tag="VNU03_23535"
     CDS             complement(3675..>4121)
                     /locus_tag="VNU03_23535"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWP77516.1"
                     /translation="LMATAALPLMLVTPAEPPSDRAPAVVPFSRAAAVGLPGAIGLMA
                     GLVGAGGAFLLVPVLVGLMHIPMRLGIGTSLAMVGMAAVTGFLGKAATGQVPLWPAAT
                     VVLGSLTGAPLGGRVSRRVPVRVLRGVLAGIIALVMLRVWHDVATH"
BASE COUNT          622 a         1479 c         1473 g          547 t
ORIGIN      
        1 tctccaagac cctcaaggac tggaagtggc cggagcacgc gcgagggcgt cgggaagccg
       61 cccatccact gcccgtccac ctcgacgccg accgacgccc cgcccttcat gaccaggtgc
      121 gaggcggcat cgggagcggc gccgtcgcgc tcggcgggcg tcagcggcgt ctcgtgcgtg
      181 ccgtagacgt tctccgcgat caggaacgcg ccgtacttgc gcatgtaggc gagcggggtc
      241 aggccctcgc gctcggccgc cgcgggcagc ccgggcacgc tatgctcgaa gatccagcgg
      301 tagaactcct cgatgcggag cttctcaccc ggccggtagg gcgactcgaa gtacttgcgg
      361 atgccgagcg agccgtcggg atcgatgcgc cacgacagct cgatccagaa ctcgtcctcc
      421 tcccacacct gcccgagccc ggccgcctcg tgggcctgcc aggtgtgatc gaaggtcttg
      481 ccctgcttct cgagcgccac ccgcagcacc ggctggcgga agccgatcca gcgcgcggcg
      541 tgggtctcct ggctcatgag gtcgtggcgc tcggaggcgt gccccatcgg cagcacgtag
      601 tccgcgaacc acgcggtctc gctccaggtc ggggtgaggc aggcgtggcg ctcgaccttg
      661 gcctcgtcgg tcagcatctc gatccacgac atgccgtcgg ggttggtcca caccgggttg
      721 tagacccgcg tgaagtacat cgcgagcttg ccgcgggcct ccttgaggaa atgcggcagc
      781 aggaagctca tctcgaagaa ggagagcgga tactcgcgcg gcatcaggag ctcgctccac
      841 accttgcccg gcggcggcat catcggcggg gccgggaccg ccttgttcca cccgctggga
      901 gcggtgccgc ccggggtggc caccgcgccc gcgaggacca cgatcagctc cagggcgcgc
      961 gccacttccc agccgtagag attgcccgcg gccgcgttgc gccacacgtg ggtggagagc
     1021 gcgctcccgg cgcggccgac ctcgcgcgcg acctcctcga tgcgggcggc cggcaccccc
     1081 gactcccgct ccgcgaactc gggggtgtag gaggcgtaga gggttttgag ggcctcctcg
     1141 aaggcggcca tggtcaccgg caggtcgggg cgctcctcgc gcaggtactc ctcccagttc
     1201 acccagcgcc ggacgaactc gcggtcccac gtgccgtcgc gcagcatgca gtgcacgatg
     1261 gccagcagca tcgcggcctc gctgccgggc cacggcgaga gccagtagtc ggccatcgac
     1321 gcggtgttgc tgagccgcgt gtcgaccacg cagatcttgg cccccgccat cttgccgtcg
     1381 atgatccgct gggcgtgcgg gttgaagtag tggccggcct cgaggtgcga ggagagcagg
     1441 aggatgaagc gcgccttgct gtggtcgggc gagggccggt cgagtccgtg ccagaaggcg
     1501 tagcccgcgc gcgcggaggc ggagcagacg ttggtgtggc tgttgtggcc gtcgatgccc
     1561 cacgcgtgga agacgcgctg gaggtagatc agctcgtggc ccggccgccc caggtggtac
     1621 atcacttcct tctgccggcc ctcgacgagc gccttccgga tgcgcccgcc gatgtcgtcg
     1681 agcaccgcgt cccaggtcac ccgctcccac ttgccctcgc cccggccggc cgcccgcctc
     1741 agcggataga ggatgcgctc cgggtccgtg atctggttga gggtggccgg gcccttcgcg
     1801 cagttgcgcc cgcggctgcc cgggtgcacc gggttgccct cgaacttgtg gaccttgagg
     1861 gactggacgt cgacgtaggc gagcagcccg caggccgcct cgcagttgaa gcagatcgtg
     1921 gggatgagct ggaagcggcg cttgaccttg cgcggccacg ccttgctgtc gagctcctcc
     1981 cagtcgtccc accgcgccgc gggcggaaac cgctccagtc ccgacgcggg cgccgccagc
     2041 gtccgcgcca ccgggtgcag cggctcgttt ggggtcaggt cttgcattac gacatttcct
     2101 cctagctgag cgggatggac tggccggcgc ggatccagag ctcttcccag agccacaggc
     2161 cagccagcgc gagccccgcg gccagctcgg agccgaacgg caccgacgcg ctcagcagca
     2221 gcacgagcgg cagcacgatg cccccgacca ccacgccgcc ccagagcgcg acccgcaggt
     2281 gcccgtgggt gagcaggcgg gccgcccgcg ccgcgtcctg cgtgccgtgc gcggtgaaca
     2341 gctccgcgaa caggaccagg gcactcacca gcagcgagcc ccagagcacg aggccgaggc
     2401 cgccggtgat gatggacgcg ctgccgagcg cgttggcggc gaggagcagg ctcgccgagc
     2461 cggccacgag cgccgccacc agcaggtggg gcagcagcag cgggctctgc cagaaatccc
     2521 ggccctcggc ctgcccgaag aggaacgcgc tgtagcccgc ggtggccgcg gccgctgcgg
     2581 cggtgggcca ggccagcagc tgcagggcca gcaggttcga catcacgccc gcgagcagcc
     2641 agagggcgcc aagcagcccg gccgcgaaca ggatgacgcc acccagcacc agccatgagc
     2701 gcgggttcgg cttgaagacc agatagagga agcgctccgg gcgcttcagg tcgaagacca
     2761 gcagcaggct ggtcaggagc aggaacgcga gcccgatcac cggcaccgcg agcgcgacca
     2821 gcgcgtcggc ggtcagcagg cccgccgcga ggccgagcga ggcgaccagg aacgcgcccg
     2881 ccgcgatcga cttcgtccac aggtacgcgg cgaccttcca cccccacggc cgcgcggggt
     2941 gcgcgacgtc atagacggtg cgcgccagcg cgagcagatc cattccgtgc gtcgccgcgg
     3001 tggccgacga gctcgcccgc gcggtcgcgg gagcgggcgg gcgattcggc tggcccgggc
     3061 tcgggatgct gccgacctgc gagaacacga acccgccgcg gcgctcctgc atgtccggcg
     3121 tgagcgcggc cgcctccgcg ccgagatagt agagcttggg ccgcgtcccc tgctccggct
     3181 tgcgcacctg ggtctgctgc gaggcgacca gccgcgagat cgtgctgtgc ggatcgtcga
     3241 gatcgcccgg gacgatggcc tggaccgggc acaccaccac gcacgccggc tcgaggccgc
     3301 tctccacccg gtgggcgcag tagtggcact tggccgcggt cttggtctcg ggatcgatgt
     3361 agagcgcgtc gtaggggcac gcctgcatgc acgacttgca gccgatgcag cggtccccgt
     3421 cgaaatcgac gatgccgtcg ggccggcgat agagcgccac cgtcgggcag atcgtcacgc
     3481 agggcgcgtt gtcgcagtgg ttgcaccgca tgacgctgaa gtagcgccgg gtgtccggga
     3541 acaccccctt ctcgatgtac ttcacccacg tgcggttcac cccgagcggg acctggttct
     3601 cctccttgca ggccaccgtg caggcgtgac agccgatgca cttgcgctgg tcgatgacga
     3661 agccgtatcg catgtcagtg ggtcgccacg tcgtgccaga cgcgcagcat gaccagcgcg
     3721 atgatacccg cgagcacccc gcgaagcacc cgcaccggca cccggcggct cacccgcccg
     3781 ccgagcggcg ccccggtcag cgagcccagc accacggtcg ccgcgggcca cagcggcacc
     3841 tgaccggtcg cggccttgcc gaggaatccg gtcaccgcgg ccatgcccac catggcgagg
     3901 gaggtgccga tgccgagccg catcgggatg tgcatcagcc cgaccagcac cgggacgagc
     3961 aggaacgcgc cgcccgcgcc cacgaggccg gccatgagcc cgatcgcgcc cgggagcccg
     4021 accgcggccg cgcggctgaa cgggaccacg gccggggcgc ggtcgctcgg gggctccgcg
     4081 ggcgtgacga gcatcagtgg gagcgccgcg gtggccatca g
//
LOCUS       DATMCV010001025         3947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_397147, whole genome shotgun sequence.
ACCESSION   DATMCV010001025 DATMCV010000000
VERSION     DATMCV010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397147"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VNU03_23540"
     CDS             complement(<1..856)
                     /locus_tag="VNU03_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWP77517.1"
                     /translation="MRDDQAGPGDTAAAAWLAGGGEMGGRIRGLDWASTPLGPTAAWP
                     QSLRSAVSILLPSKAQICLFWGPDLVKLYNDAYIPVLGQKHPGMLGRPAREVWSEIWD
                     ILGPLLRGVIATGDAFRGVDYPFYLERHGFTEETFFDVSYDPVRDESGKVGGVFCIVS
                     ETTGRVLSERRLRTLRDLGRAREGRSPAEACTLALGALASNPRDVPSVSLYLLDDGGA
                     VAHAVGSVGIPAGGEWSPLDIALADPRWDLATIAQSGRPQVLEAGSAGAGSVAGPPER
                     TLIHPILRA"
     gene            complement(928..2022)
                     /locus_tag="VNU03_23545"
     CDS             complement(928..2022)
                     /locus_tag="VNU03_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWP77518.1"
                     /translation="MTTLHTPFCDLVGIEYPIVQAPMAGGPTTPELVAAVSAAGALGS
                     FGHAYAAADAMRADAAAVRARTARPFNLNLFVAPVAAEAPLEQQRAAIAAVRPLFERH
                     GLAVPERVPPPYAPDLATQLEAVCDLRPAVCTIHLGDLSPAVLARIRGLGIRLGSAAT
                     SVREARHLEGLGADFIIAQGGEAGGHRGTFLGPWEQAMTGTLALVRQIVRAVRVPVVA
                     AGGIMDGAGVAAVLALGAQAAQLGTAFVVCPESGAPAPHKKAIAQMDGDETTITRAFS
                     GKPARGVRNQFIEAAERERWPLLPFPAHGKLTAPLRQASARAGSPDLFSAWSGQAGSL
                     ARPLPAAELVRVLVDEAREVIGHLRRITGG"
     gene            2079..2954
                     /locus_tag="VNU03_23550"
     CDS             2079..2954
                     /locus_tag="VNU03_23550"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77519.1"
                     /translation="MLGLDVILQAGLSGLFMGSVYALIAIGFTLVFGVTNIVNFAHGH
                     FVMGAMFVTYLLFKTLKVDPYVGLVVVLPLFFVLGSLIYRLVIQRIVEAPHSAHMMVT
                     LGLLIFIENLANLFFGGDLRGITTTYTTSSLVLGDVSFPVARMGAAAVSLAAVVGLAL
                     VLHRTSLGKAIRAAANNPEGAALVGIDVGRVYRVAFSLGTAAAALAGAVIMPFSLVSP
                     FVGGEFILKAFVIAVLGGLGSVAGALIGGLLIGLVEALSSLYISASLGNAIVFAILIA
                     VLLYRPWGILGQARV"
     gene            2995..3921
                     /locus_tag="VNU03_23555"
     CDS             2995..3921
                     /locus_tag="VNU03_23555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77520.1"
                     /translation="MAALAIAAALPLAAGPHLLTAMIVALHAAFMALAWNVAAGYAGQ
                     FSLGHSLFYGIGAYASTMLYLRLGLTPWAGMFVGAALAGIVGALLALGVYRYKVHGIF
                     FALVTLGAAEVAKGLADNWAFIKGPVGILLTMQNAPGDFFFLRREPYYYVALTMLAAM
                     IVISLLLERSPLGQYFLAVREDEQAAEASGVDSYRYKTIAISLSAALTAFAGSFYAQF
                     YLYVSPDTVLVFEPQLTMMLGTMVGGAGTAFGPVIGSLLFSGLGEALRNLPFLESTRG
                     VVIGSKMVYAVLLIVVLIYLPGGLITLGRKGA"
BASE COUNT          504 a         1462 c         1361 g          620 t
ORIGIN      
        1 cggctctcag gatggggtgg atcagcgtcc gctccggcgg gccggccacg gacccggccc
       61 cggcggatcc cgcctccagc acctgcggcc ggccgctctg cgcgatcgtc gccagatccc
      121 accggggatc ggcgagcgcg atgtcgagcg ggctccactc cccgccggcg gggatcccga
      181 ccgaccccac cgcgtgcgcg accgcgccgc cgtcgtcgag gagatacagg ctcacggacg
      241 gcacgtccct cggattggac gcgagagcgc ccagcgccag ggtgcaggcc tccgccgggg
      301 accggccttc ccgggcgcgc cccaggtcgc gcagcgtacg gaggcggcgc tcgctcagga
      361 cccggccggt ggtctcgctg acgatgcaga acacgccgcc caccttgccc gactcgtccc
      421 gcaccgggtc gtacgagacg tcgaagaacg tctcctcggt gaacccgtgc cgctccaggt
      481 agaaggggta gtccaccccg cgaaacgcgt cgccggtggc gatgacgccc cggagcagcg
      541 gcccgaggat gtcccagatc tcgctccaga cctcccgggc gggccgcccg agcatcccgg
      601 gatgcttctg gccaagcacc gggatgtagg cgtcgttgta gagcttgacc aggtccgggc
      661 cccagaacag gcagatctgc gccttggagg gaaggaggat gctgaccgcg ctccggagac
      721 tctgcggcca ggcggcggtg ggcccgaggg gggtcgaggc ccagtcgagg ccccgaatgc
      781 gcccgcccat ctcgccaccg cccgcgagcc acgcggctgc tgcggtatcg ccggggccgg
      841 cttggtcgtc gcgcatgatc caggcgcgac gagtatacct acgtttacgg gctcacgcgg
      901 cgccgggtgc tcgcgcgggg cggcacgtca tcccccggtg attcgtcgca ggtgaccgat
      961 cacctcgcga gcttcgtcga ccaggacgcg gaccagctcc gcggcgggaa gcgggcgggc
     1021 gagcgagccc gcttgcccgg accacgccga gaacaggtcc ggcgagccgg cgcgcgcgct
     1081 ggcctggcgc agcggggcgg tcagcttgcc gtgggccggg aacggcagaa ggggccacct
     1141 ctcgcgctcg gccgcctcga tgaactggtt acgcacgccc cgcgccggct tgcccgagaa
     1201 ggcccgcgtg atggtggtct cgtcgccgtc catctgggcg atggccttct tgtgcggtgc
     1261 gggcgcgccg ctctccgggc acaccacgaa ggcggtgccg agctgcgccg cctgcgctcc
     1321 cagcgccagc accgcggcga cgcccgcgcc gtccatgatg ccgcccgcgg ccaccaccgg
     1381 cacgcgcacc gcgcgcacga tctgccggac gagggcgagc gtcccggtca tggcctgctc
     1441 ccacggcccc aggaacgtgc cgcggtgtcc gccggcctcg ccaccctggg cgatgatgaa
     1501 atccgcccct aggccctcca ggtggcgcgc ctcgcggacc gaggtggccg cgctgccgag
     1561 gcggatgccg agaccgcgga tgcgggcgag caccgcgggc gacagatcgc ccaggtggat
     1621 ggtgcatacc gcgggccgca ggtcgcacac cgcctcgagc tgggtggcca ggtcgggcgc
     1681 gtacgggggc ggcacgcgct cgggcaccgc gaggccgtgc cgctcgaaga gcggccggac
     1741 cgcggcgatc gccgcccgtt gctgctcgag cggcgcctcg gcggccacgg gggcgacgaa
     1801 gagattcagg ttgaagggcc gcgcggtgcg cgcccgcacc gcggccgcgt ccgcgcgcat
     1861 ggcgtccgcc gcggcatagg cgtggccgaa cgagccgagg gccccggccg cgctcaccgc
     1921 ggcgacgagc tccggggtgg tgggcccgcc cgccatcggg gcctggacga tcgggtactc
     1981 gatgccgacg agatcgcaga aaggagtatg cagcgtcgtc atgtccgcat gatagcccgc
     2041 cttgacaccc gcgcgccccc ctgcaatagt ttgccggcat gctcggcctc gacgtgattc
     2101 tccaggccgg cctctccggt ctcttcatgg gcagcgtgta cgcgctcatc gccatcggct
     2161 tcaccctcgt cttcggcgtc accaacatcg tgaacttcgc gcacggccac ttcgtcatgg
     2221 gcgcgatgtt cgtcacctac ctcctgttca agaccctcaa ggtggatccc tacgtcggcc
     2281 tcgtggtggt gctcccgctg ttcttcgtgc tggggtccct gatctaccgg ctcgtgatcc
     2341 agcgcatcgt ggaggccccc cactcggccc acatgatggt gaccctcggc ctgctcatct
     2401 tcatcgagaa cctcgccaac ctcttcttcg gcggcgatct tcgcggcatc accacgacgt
     2461 acaccacgtc gtccctcgtg ctcggcgacg tctccttccc cgtcgcgcgc atgggcgccg
     2521 ccgcggtcag cctcgccgcg gtggtggggc tcgccctcgt cctccaccgc acgtccctgg
     2581 gcaaggccat tcgcgccgcg gccaacaacc cggagggagc cgccctcgtg gggatcgacg
     2641 tcgggcgcgt gtaccgggtc gccttctccc tgggaaccgc cgccgccgcg ctcgccggcg
     2701 cggtgatcat gcccttctcg ctcgtgagcc cgttcgtcgg cggcgagttc atcctgaagg
     2761 ccttcgtcat cgcggtgctg ggcgggctcg gcagcgtggc gggcgcgctc atcggagggc
     2821 tcctcatcgg gctcgtggag gcgctctcca gcctgtacat ctcggcctcg ctcggcaacg
     2881 ccatcgtgtt cgcgatcctg atcgcggtcc tgctctaccg tccgtggggc atcctcggtc
     2941 aggcccgggt gtgacgcgct gagcgggcat ccggcgcgct tcatcggaat cgcggtggcc
     3001 gcgctggcca tcgccgcggc gctgccgctc gcggccggtc cgcatctgct caccgcgatg
     3061 atcgtggcgc tgcacgccgc gttcatggcg ctggcctgga acgtggcggc cggctacgcg
     3121 ggccagttct cgctcggcca cagcctcttc tacggcatcg gcgcctacgc ctccacgatg
     3181 ctctacctgc gcttaggcct gaccccgtgg gccggcatgt tcgtgggcgc cgccctcgcg
     3241 gggatcgtgg gcgccctgct cgccctgggg gtctaccgct acaaggtgca cggcatcttc
     3301 ttcgcgctgg tgaccctcgg ggcggccgag gtggctaagg ggctcgccga caactgggcc
     3361 ttcatcaagg gaccggtggg gatcctgctc accatgcaga acgcgccggg cgacttcttc
     3421 ttcctccggc gggagccgta ctactacgtc gcgctcacca tgttggccgc gatgatcgtg
     3481 atcagcctgc tcctggagcg ctcgcccctg ggacagtact tcctcgcggt gcgcgaggac
     3541 gagcaggccg ccgaggcctc cggcgtcgat tcgtaccgct acaagaccat cgccatcagt
     3601 ctctcggccg ccctcaccgc gttcgccggg agcttctacg cccagttcta tctctacgtg
     3661 tcgcccgaca cggtcctggt cttcgagccg cagctcacca tgatgctcgg taccatggtc
     3721 gggggcgcgg gcaccgcctt cggacccgtc atcgggtctc tgctcttcag cgggctcggc
     3781 gaggcgctcc gcaacctgcc cttcctcgag agcacgcgcg gggtcgtcat cgggagcaag
     3841 atggtctacg cggtcctgct catcgtggtc ctcatctacc tgcccggcgg tctcatcacc
     3901 ctcgggcgga aaggggcgtg aggccggatg gccacgctgc tcgacgt
//
LOCUS       DATMCV010001026         6749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_720698, whole genome shotgun sequence.
ACCESSION   DATMCV010001026 DATMCV010000000
VERSION     DATMCV010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720698"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VNU03_23560"
     CDS             complement(<1..387)
                     /locus_tag="VNU03_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199345.1"
                     /note="with UgpACE is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWP77521.1"
                     /translation="MPETKTSWWSAGAALLLATALLLVPATPALAKTDIQFWHAMTGQ
                     LNDAVNALVKQFNESQAEYEVKPLQKGNYAETLTAAIAAYRQRQAPHIVQVFEVGTQT
                     MMLSGAILPVYQLMKEQEIAVDWKDFI"
     gene            complement(521..880)
                     /locus_tag="VNU03_23565"
     CDS             complement(521..880)
                     /locus_tag="VNU03_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77522.1"
                     /translation="MTRRRLRVALALLALLLLAAAPVPAADQAWLVGRWELLRDPDGG
                     AQDWLEFNREGQVALAKRNGPRYTGRYHASDDAVQIDYKIGSQSVITTLTPSSDKREL
                     YARSAVTGMNAVYEKRP"
     gene            complement(877..2511)
                     /locus_tag="VNU03_23570"
     CDS             complement(877..2511)
                     /locus_tag="VNU03_23570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HWP77523.1"
                     /translation="MPANRLAGLDGAALDAVVIGGGMAGAGVARDLALRGASVALFEK
                     GDFASGTSSKSSKLIHGGLRYLELGDFKLVRESLREKKTLERLAPHLVRPLPFLVPIY
                     RGAPRGLITVRIGMWLYDLLTPGKTTERYRVLRPVDALTMEPSVRAEDLRGAGYYFDD
                     LLLYPERLCLENVLSAARHGARTFNYCEVEEFVHGRRGIEGVKVRDLLSGQVQLVRAA
                     TIVNCAGPWVDRVRAMAKVADRSPRVVRTTKGIHCLLPRMTDRAVYLSARDERMIFVI
                     PWREFSLVGTTDTDFEGDPDRLWATREEVTYLLEEVAKVLPDKRATFDNVSYTYAGVR
                     PLSFEPGASASKVSRDHKVIPEGPDGRFFSVTGTKLTCFRSLAEDVGNRVMRALGRAQ
                     APRTARLTLDGADEEVGKIEARVWMDVSEEMAATGLSRGTLRTLVETYGRAYPRVLEL
                     GRKLPDGFERLCPSNPEIVAQLHHALREELAVSLQDVLLRRTGIGQSRCQGLDCAESI
                     AARMAELGGWSRRRLDAELEAYSQHVERSRRFRQRA"
     gene            complement(2522..3310)
                     /locus_tag="VNU03_23575"
     CDS             complement(2522..3310)
                     /locus_tag="VNU03_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HWP77524.1"
                     /translation="MRVVVPKLPYRGWLFDLDGTIYLGERLIPGADAAIAALRAAGRR
                     VAFLSNKPLQTRAEYAAKLTRLGVPAEPDDVINSSLVLARYLRDRDPGAPVFVIGEPP
                     MRDEMRAHGFEVRSDERVRWVVIAFDRTFDYAKLNVALQAVKQGARLIATNPDRTCPV
                     EGGEIPDCGGMIAAVEAVTDKKVEAIVGKPSPIILEVALAALGVPARDAAIVGDRIET
                     DIVMGRRLGLGTVLVLSGVTRADDPRIAAVAPDHVVGSVRDLVE"
     gene            complement(3354..4835)
                     /locus_tag="VNU03_23580"
     CDS             complement(3354..4835)
                     /locus_tag="VNU03_23580"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWP77525.1"
                     /translation="MNPALPALVFPSVDLRPILPMLVIALTAGVVLVLDLLPPRERKD
                     HLGFVSALGVVAALIVTYWMTFSTGGADLRGFRGMVVLDGFALFFNIVIGYAAGLVLL
                     LSMDYIRREGEEAGEFYILVLLSALGMMLMASAGDLIIVFLGLETMSISLYVLAGFFR
                     TRLEAGEASMKYLLIGAFASGFFLYGIALIFGATGTTNLDRIANAVTAGAGRDPLLII
                     GLGLLLVGFGFKISSVPFHMWAPDVYEGAPTSVTAFIATGSKAAAFAALLRVLLTAVR
                     GAPADWPMLLWVVAALTMTVGNVVALAQQNLKRMLAYSSIAHVGYMLVGVVAGGAFGN
                     GSVLFYLLAYTFTTAGAFGTILLLERAGQEAVRLDDFGGLAARHPLIAVALSVFLLSL
                     VGIPPTAGFVGKFYLFGAAVKSGYVWLAVIGVLNSAVAAYYYLRLIVYMYMREPEGAP
                     TVLAPSFSGALALAVALWGVVQLGVAPGPLFDLAQAAVVPLLR"
     gene            complement(4832..6367)
                     /locus_tag="VNU03_23585"
     CDS             complement(4832..6367)
                     /locus_tag="VNU03_23585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWP77526.1"
                     /translation="MAYLLSLVTFVPMLGAIVVLLLPRRQEHVTKLATLATTLVTFAI
                     SLPVYFRFDAASADYQFVEQRAWIPSLGVTYHVGVDGISVLLVLLTTFLMPLVLLSSW
                     TSVESRWKEFAITMLLLETGMLGVFVALDLFLFYVFWEAMLIPMYLIIGIWGGPNRVY
                     AAVKFILYTLAGSLLMLVAILFVYFQHGAATNVYTFDLPVLVRFVSPGGLTQDLLFLA
                     FALAFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFCLPLFPQASL
                     AWAPWIFTLSIIGILYGAWVSTVQPDIKKLVAYSSVSHLGFVVLGIFTLTPQGMVGGI
                     IQMVSHGLSTGALFLMVGMIYERRHTRLIADFGGLWKVIPAFSVLFLIVCFSSMGLPG
                     LNGFVGEFLILVGTFQVHRLAAVLATSGIIFAAVYLLWMYQRVCFGEVTSEANRRLAD
                     LSPREWAVLLPVLLFIVWIGVYPTTFTTMTEPSVQALITQVQTKAQATSAMLMSPHVP
                     VTR"
     gene            complement(6373..>6749)
                     /locus_tag="VNU03_23590"
     CDS             complement(6373..>6749)
                     /locus_tag="VNU03_23590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWP77527.1"
                     /translation="TLLVLSALVAIAGVALAWMVYGRTPVRAASIGVARNPLHKLLIE
                     KYYVDEIYDALFVKPIYRLSLWLARVFDPGLIDGIVNGVATVVAGWARGLRRVQTGYV
                     MNYALGILLGAVAVVAYLLARR"
BASE COUNT         1150 a         2316 c         2402 g          881 t
ORIGIN      
        1 gatgaagtcc ttccagtcga ccgcgatctc ctgctccttc atgagctggt agaccggcag
       61 gatcgccccg gagagcatca tggtctgggt cccgacctcg aacacctgca cgatgtgggg
      121 cgcctgccgc tggcggtagg ccgcgatcgc ggcggtgagg gtctccgcgt agttgccctt
      181 ctggagcggc ttcacctcgt actcggcctg gctctcgttg aactgcttca cgagcgcgtt
      241 gaccgcgtcg ttcagctggc cggtcatcgc gtgccagaac tggatatcgg tcttcgcgag
      301 cgcgggggtg gccgggacca gcagcaatgc ggtggcgagc agcagcgcgg ctcccgccga
      361 ccaccaggac gtcttcgtct cgggcatggg tgacctccgg gttgtcaggg tagcggctcg
      421 gcgtctcgat cgggctgcgc gcggatgacg ttctcacgac gcccgcggcg gcgctcggaa
      481 ctatagcatc attggcgcgc ggccgggaag cggcgggcgg ctacggccgc ttctcgtaca
      541 ccgcgttcat gccggtcacc gccgagcggg cgtagagctc ccgcttgtcc gagctggggg
      601 tcagggtggt gatcaccgac tggctgccga tcttgtagtc gatctggacc gcgtcgtcgg
      661 aggcgtggta gcggccggtg tagcgcgggc cgttgcgctt ggccagggcc acctggccct
      721 cgcggttgaa ctcgagccag tcctgcgcgc cgccgtccgg gtcccgcagc agctcccagc
      781 gcccgacgag ccaggcctgg tcggcggccg ggaccggtgc ggcggcgagc agcaggagcg
      841 cgaggagagc gagggcgacg cgcaggcgtc gccgcgtcat gcgcgctgcc ggaagcggcg
      901 gctgcgctcg acgtgctggg agtaagcctc gagctccgcg tcgagcctgc gcctggacca
      961 gccgcccagc tcggccatgc gcgccgcgat ggactccgcg cagtcgaggc cctggcagcg
     1021 gctctggccg atgccggtgc ggcgcagcag cacgtcctgc agcgagacgg ccagctcctc
     1081 gcggagggcg tggtggagct gggccacgat ctcgggattg ctcgggcaca ggcgctcgaa
     1141 gccgtcgggc agcttgcgcc ccagctcgag cacgcgcgga taggcacggc cgtaggtctc
     1201 caccagcgtg cgcagtgtac cgcgcgagag cccggtggcc gccatctctt cggagacgtc
     1261 catccacacc cgcgcctcga tcttgccgac ctcctcgtcc gcgccgtcga gggtgagccg
     1321 cgcggtgcgc ggggcctggg cgcggccgag cgcgcgcatg acccggttgc ccacgtcctc
     1381 cgcgaggctg cggaagcagg tcagcttggt cccggtcacc gagaagaagc ggccgtcggg
     1441 gccctccggg atcaccttgt ggtcgcgcga gaccttggac gcggaggccc ccggctcgaa
     1501 ggagagcggc cgcacccccg cgtaggtata ggagacgttg tcgaaggtgg cgcgcttgtc
     1561 gggcagcacc ttggccactt cctccaggag gtaggtcact tcctcgcggg tcgcccacag
     1621 ccggtccgga tcgccctcga agtcggtgtc ggtggtgccc accagcgaga actcgcgcca
     1681 cgggatcacg aagatcatcc gctcgtcgcg ggccgagagg tacaccgccc ggtcggtcat
     1741 gcgcgggagc aggcagtgga tgcccttggt ggtgcgcacc acccgcgggc tccggtcggc
     1801 caccttcgcc atcgcgcgca cccggtcgac ccagggcccc gcgcagttga cgatggtggc
     1861 ggcccgcacg agctgcacct ggccggacag caggtcgcgc accttcacgc cctcgatccc
     1921 ccggcggccg tgcacgaact cctcgacctc gcagtagttg aaggtccgcg ccccgtgccg
     1981 cgcggccgag agcacgttct ccaggcagag ccgctccgga tagagcagca ggtcgtcgaa
     2041 gtagtagccc gccccgcgca ggtcctccgc ccgcaccgag ggctccatgg tgagcgcgtc
     2101 gaccggccgg agcacccggt agcgctcggt ggtcttgccc ggggtgagca gatcgtagag
     2161 ccacatgccg atgcgcaccg tgatgaggcc gcgcggcgcg ccgcgataga tgggcaccag
     2221 gaacggcagg gggcggacca ggtggggggc gagccgctcg agcgtcttct tctcgcgcag
     2281 cgactcgcgc accagcttga agtcgcccag ctcgaggtag cggagcccgc cgtggatcag
     2341 cttggaggac ttcgacgagg tgccggaggc gaaatcgcct ttctcgaaga gcgcgaccga
     2401 cgcgccacgc agggccaggt cgcgcgccac ccccgccccc gccatgccgc cgccgatcac
     2461 cacggcgtcc agcgcggcgc cgtcgagccc agccagtcgg ttcgcgggca cgctcactcg
     2521 atcactccac caggtcccgg accgatccta ccacgtggtc gggagcgacc gcggcgattc
     2581 gggggtcgtc cgcgcgcgtg acgccgctca gcaccagcac ggtgccgagc ccgagccgcc
     2641 ggcccatcac gatgtcggtc tcgatgcggt cgcccacgat ggccgcgtcg cgggcgggaa
     2701 ctcccagcgc ggcgagcgcg acttccagaa taatcggaga tggtttcccc acgatcgcct
     2761 cgaccttctt gtcggtcacc gcctccaccg ccgcgatcat ccccccgcag tccggaatct
     2821 cgccgccttc caccgggcag gtgcgatcgg gattggtggc gatgagccgc gccccctgct
     2881 tcaccgcctg gagcgcgacg ttcagcttcg cgtagtcgaa ggtgcggtcg aaggcgatca
     2941 ccacccagcg cacccgctcg tcggaccgca cctcgaagcc gtgggcgcgc atctcgtccc
     3001 gcatcggcgg ctcgccgatc acgaagaccg gcgcgccggg atcccggtcg cggaggtagc
     3061 gcgcgagcac cagggaggag ttgatgacgt cgtccggctc ggcggggacc cccaggcggg
     3121 tgagcttggc cgcgtactcc gcccgggtct ggagcggctt gttggagagg aacgcgaccc
     3181 ggcggcccgc cgcccgcagc gccgcgatgg ccgcgtcggc cccggggatc agccgctcgc
     3241 cgaggtagat cgtgccgtcg aggtcgaaga gccagccgcg gtaggggagc ttggggacca
     3301 ccaccctcac cttttctccc ctcaccctac cctctcccct gaggggagag ggttcaacgc
     3361 agcagcggga cgacggcggc ctgggccagg tcgaacagcg ggcccggcgc caccccgagc
     3421 tgcaccacgc cccacagcgc gacggccagc gcgagcgcgc ccgagaacga cggggcgagc
     3481 acggtgggcg cgccctccgg ctcccgcatg tacatgtaga cgatgaggcg caggtagtag
     3541 taggcggcga ccgcggagtt gagcacgccg atcacggcca gccagacgta gcccgacttc
     3601 accgcggccc cgaagaggta gaacttgccc acgaagcccg cggtgggcgg gatgccgacc
     3661 agggagagca ggaacaccga gagggccacc gcgatcagcg ggtgccgtgc cgcgagcccg
     3721 ccgaagtcgt cgaggcgcac cgcctcctgg cccgcccgct ccagcagcag gatcgtcccg
     3781 aaggcccccg cggtggtgaa ggtgtacgcg agcagataga agagcacgct gccgttgccg
     3841 aaggccccgc ccgccaccac ccccaccagc atgtagccca cgtgggcgat cgaggagtat
     3901 gcgagcatgc gcttgagatt ctgctgggcg agagccacca cgttgcccac cgtcatggtg
     3961 agcgcggcga ccacccacag cagcatcggc cagtccgccg gggcgccgcg caccgcggtg
     4021 agcagcacgc ggaggagcgc ggcgaaggcg gccgccttgg agccggtcgc gatgaacgcg
     4081 gtcaccgagg tgggcgcgcc ctcgtacacg tcgggcgccc acatgtggaa gggcaccgag
     4141 gagatcttga agccgaagcc caccagcagg agaccgagcc cgatgatcag cagcgggtcg
     4201 cggcccgccc ccgccgtcac cgcgttggcg atgcgatcga ggttcgtggt gccggtggct
     4261 ccgaagatga gggcgatgcc gtagaggaag aagccggagg cgaaggcccc gatcagcagg
     4321 tacttcatgg aggcctcgcc cgcctcgagg cgggtccgga agaagccggc cagcacgtag
     4381 agcgagatcg acatcgtctc gagcccgaga aagacgatga tcaggtcgcc cgcggaggcc
     4441 atcagcatca tgccgagcgc ggagagcagc accaggatgt agaactcgcc cgcctcctcg
     4501 ccctcgcgcc ggatgtagtc catcgagagc aggagcacga gcccggccgc gtagccgatc
     4561 acgatgttga agaagagcgc gaagccgtcc aggaccacca tgccgcggaa gccccgcagg
     4621 tcggccccgc cggtggagaa ggtcatccag taggtgacga tcagggccgc caccaccccg
     4681 agcgcgctca cgaagcccag gtggtccttg cgctcccgcg ggggaagcag gtcgaggacc
     4741 agcaccacgc ccgcggtgag cgcgatcacc agcatcggca ggatcggccg caggtcgacg
     4801 gacgggaaga ccagcgcggg aagcgcgggg ttcatcgcgt gaccgggacg tggggggaca
     4861 tcagcatggc cgaggtcgcc tgcgccttgg tctgcacctg ggtgatgagg gcctgcacgc
     4921 tcggctcggt catggtggtg aaggtggtcg ggtagacccc gatccacacg atgaagagca
     4981 gcaccggcag gagcaccgcc cactcccgcg ggctcaggtc cgcgaggcgc cggttcgcct
     5041 cggaggtcac ctcgccgaag cacacccgct ggtacatcca gagcaggtac accgcggcga
     5101 agatgatgcc gctcgtggcg aggaccgcgg cgagccggtg cacttggaag gtgccgacca
     5161 ggatgaggaa ctcccccacg aagccgttca ggccggggag ccccatcgag gagaagcaca
     5221 cgatcaggaa cagcacgctg aaggcgggga tcaccttcca gagcccgccg aagtcggcga
     5281 tgagcctcgt gtgccgccgc tcgtagatca tgccgaccat gaggaagagc gcgccggtgc
     5341 tgagcccgtg gctcaccatc tggatgatgc cgcccaccat gccctgcggg gtcagggtga
     5401 agatgcccag caccacgaag cccaggtggc tcacgctcga gtaggccacc agcttcttga
     5461 tgtccggctg cacggtggag acccacgccc cgtagaggat gccgatgatc gagagggtga
     5521 agatccacgg ggcccacgcc aggctggcct gcgggaacaa cggcaggcag aagcgcagga
     5581 agccgtaggt ccccatcttc aggagcaccc cggccaggat cacgctgccc gcggtggggg
     5641 cctccacgtg ggcgtccggc agccaggtgt ggaacgggaa catcggcacc ttgatcgcga
     5701 aggcgagcgc gaaggccaga aagagcaggt cctgggtcag cccgcccggg ctgacgaagc
     5761 gcaccagcac cggcaggtcg aaggtgtaga cgttggtggc cgccccgtgc tggaagtaga
     5821 cgaacaggat ggccaccagc atcaggagcg agccggccag cgtatagagg atgaacttga
     5881 ccgccgcgta gacgcgattc ggcccacccc agatcccgat gatcaggtac atcgggatca
     5941 gcatcgcctc ccagaagacg tagaagagga agaggtcgag ggccacgaag accccgagca
     6001 tccccgtctc caggagcagc atggtgatcg cgaactcctt ccagcgcgac tccaccgacg
     6061 tccacgagga gagcagcacc agcggcatca ggaaggtggt cagcagcacc agcagcacgc
     6121 tgatgccgtc cacccccacg tggtaggtga ccccgagcga ggggatccac gcccgctgct
     6181 ccacgaactg gtagtccgcc gacgcggcgt cgaagcggaa gtacaccggc agcgagatcg
     6241 cgaaggtcac cagggtcgtg gcgagggtgg ccagcttggt gacgtgctcc tggcgccggg
     6301 gcagcagcag gacgacgatc gccccgagca tcgggacgaa ggtgacgagc gagaggaggt
     6361 acgccatgcg ggctagcggc gcgcgagcag gtaggcgacc accgcgaccg cgcccagcag
     6421 gatgccgagc gcgtagttca tcacgtagcc ggtctgcacc cggcggagcc cgcgcgccca
     6481 gcccgccacc accgtcgcga ccccgttgac gatgccgtcg atgaggccgg gatcgaagac
     6541 gcgggccagc cagagcgaga ggcggtagat cggcttcacg aagagcgcgt cgtagatctc
     6601 gtccacgtag tacttctcga tcagcagctt gtggagcggg ttgcgcgcca ccccgatgga
     6661 ggcggcccgc accggggtgc ggccgtagac catccaggcc agcgccaccc ccgcgatggc
     6721 cacgagggcc gagagcacca gcagggtga
//
LOCUS       DATMCV010001027         2877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_720851, whole genome shotgun sequence.
ACCESSION   DATMCV010001027 DATMCV010000000
VERSION     DATMCV010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720851"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VNU03_23595"
     CDS             <1..581
                     /locus_tag="VNU03_23595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWP77528.1"
                     /translation="ARRLCGLPENARVLLTVGGVYTGKGQHTVVDLLPSLMARYPEAL
                     YVMVGKPRPGETYERRLRETVAKLGLDGHVLFAGPRPHAELRRWFTAADVSVLATQSE
                     GWPNVLLESIACGTPVVATHVGGAPEIVRHGKDGFLVPYGATAALRDALLMAMEQPWD
                     REALVRYAQRFDWSESVDQALAELEAALKESP"
     gene            578..1957
                     /locus_tag="VNU03_23600"
     CDS             578..1957
                     /locus_tag="VNU03_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HWP77529.1"
                     /translation="MNERLVRQFLFPFHERLKRKPTFQHLKALGESQWLVPTQLVELQ
                     VRRLRQLLEFAHDHVPYYRALFEEHSLSPRHVHSMDDLSHLPFLTRDLVQTRSNDLRA
                     RASLPGMHTRSSGGSTGAPVTVVVDMGRMGMGEAGRLRAQGWFGVEPGAREIVLWGVP
                     LRVDRTERIRRVRDWFLNTRVLSAFDMGETALRSHARTIQRARPTKMYGYASAFHLIA
                     GYFERERLQPPAGLRAVFTTAEPLFDFQRKTIEAALGCAVATEYGCRDGGLVALECPD
                     GGLHTFGESMLVEILDPDPEGRGEIVLTNLDSHAFPMIRYRTGDIGSLDPTPCRCGRT
                     LPKLRHVEGRRTDFLVTPAGRVLHALSAIYVLRETPAVREFRIVQERADHVTVSVVPD
                     PAFGRAEDAAIRSGLTALLGPDTRVEVETVPRIPRTASGKYRYVESRVAQDVIERLMR
                     APSFRSSEP"
     gene            1954..>2877
                     /locus_tag="VNU03_23605"
     CDS             1954..>2877
                     /locus_tag="VNU03_23605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWP77530.1"
                     /translation="MSDPLKICVVGTGHIGLPLAAVLADAGFSVVGYDTNDDFVTRVN
                     TTGTVDFQEEGLSELLAKHLHRGLTLTSTPPDRQDIYVITVGTPLEVGQERPNLDRIR
                     VAVQRIAPGFGENPLVVLRSTVSIGTTRRIVLPEIRCYTERFGLAFCPERTIEGRAIP
                     EMRSLPQIIGGVDAESADRAEALFRRITESIVRVSSLEAAEMIKLINNTYRDLTFAFA
                     NEVALIAEHMGLSAREMIHAANVNYPRSGVARPGFVGGPCLEKDALILIDSLHQIDFK
                     PRVIEEARRINHALPEHVAARVLEQLQQLRGS"
BASE COUNT          439 a         1007 c          969 g          462 t
ORIGIN      
        1 aggcccggcg actctgcggc ctcccagaga acgcacgagt gctcctgacg gtcggcgggg
       61 tctacaccgg caagggccaa catacggtag tggatctcct gccgtccttg atggcgcgat
      121 atcccgaagc cctgtacgtg atggtgggca aaccccgtcc cggggagacg tacgagcggc
      181 gtctccggga gaccgtcgcg aagctcggac tagacgggca cgtgctcttt gccgggccgc
      241 gaccccatgc cgagcttcgt cgctggttca ccgcggccga tgtgtctgtc ctggcgacgc
      301 agtctgaagg ctggccaaac gttctcctcg aatcgatagc ctgcgggacg cccgtggtcg
      361 ccacgcacgt tggaggggca ccggagattg ttcggcatgg gaaggacggc tttctcgtcc
      421 cgtacggggc gaccgccgcg ctgcgcgacg cgttgctcat ggcgatggag cagccatggg
      481 atagggaggc cctcgtgcgc tatgcgcagc gcttcgactg gagcgagagc gtggaccagg
      541 cgctcgcgga gctcgaagcg gccctgaaag agtcgccgtg aacgagcggc tggttcgcca
      601 gttcctcttc ccgttccatg agcgcctcaa gcgcaagccc acgtttcagc acctgaaggc
      661 gctcggggag agccagtggc ttgtcccgac gcagcttgtg gagcttcagg tccggcggct
      721 gcggcagcta ctggaattcg cgcacgacca tgtgccgtac taccgcgcgt tgttcgagga
      781 gcactcgctg tcgccgcgac acgtccactc gatggacgac ctcagtcatc ttccgttcct
      841 cacccgagac cttgttcaga cgcgatcaaa cgaccttcgg gcccgggcat ccctgccggg
      901 aatgcacacc cgctcctccg gcgggtccac gggagcaccc gtcacggtcg tggtggacat
      961 gggtcgcatg ggcatgggcg aggctggccg cctccgggct cagggatggt tcggggtcga
     1021 gccgggcgcc cgcgagatcg tgctgtgggg ggttccgctc cgggtcgacc ggacggagcg
     1081 tatccgtcgg gtgcgggact ggttcctgaa cacccgggtc ctgtccgcct ttgacatggg
     1141 cgagacggcg ctccggtcgc acgcgcgtac gatccagcgc gcccggccga ccaagatgta
     1201 cggctacgcg agcgctttcc atctcatcgc cggctacttc gagcgggagc ggctccagcc
     1261 gccggccggc ctgcgcgccg tcttcacgac cgcggagccg ctgttcgact tccagcgcaa
     1321 gaccatcgag gcggcgctgg gctgcgcggt cgcgaccgag tacggctgcc gggacggcgg
     1381 cctggtcgcg ctggagtgcc cggacggcgg gctgcacacc ttcggggaga gcatgctcgt
     1441 cgagatcctc gacccggatc cggagggccg cggagagatc gtgctcacga acctcgactc
     1501 ccacgcgttc ccgatgatcc gctaccggac cggagacatc ggcagtctcg acccgacccc
     1561 gtgccggtgc ggccgcaccc tgccgaagct ccgtcacgtg gagggccgtc gcaccgactt
     1621 tctggtcacc cccgcgggcc gggtgctcca cgccctttcc gcgatctacg tgctgcgcga
     1681 gacgccggcg gtgcgcgagt tccgcatcgt gcaggagcgg gccgaccacg tgaccgtcag
     1741 cgtggtgccg gatcccgcgt tcggccgggc cgaggacgcg gccatccgga gcgggctcac
     1801 cgcgctcctg ggcccggaca cccgggtcga ggtggagacc gtgccgagga ttccccgtac
     1861 cgcgtccggc aagtaccgct atgtcgaatc ccgcgtcgcc caggatgtca tcgagcgcct
     1921 gatgcgagcg ccttcattta ggagcagcga gccatgagcg atcccctgaa gatctgtgtc
     1981 gtcggcaccg gccacatcgg ccttcctttg gcggccgtgc tggccgacgc gggattctcc
     2041 gtcgtcggct acgacaccaa cgacgacttc gtcacccggg tgaacaccac cggtaccgtc
     2101 gacttccagg aagagggcct gagcgagctc ctggccaagc acctgcaccg gggcctcacc
     2161 ctgacctcca cgccgcccga ccggcaggac atctacgtca tcacggtggg caccccgctc
     2221 gaggtgggtc aggagcggcc caatctcgat cggatccgcg tggcggtgca gcgcatcgcg
     2281 cctggcttcg gcgagaatcc gctcgtggtg ctccggagca cggtgagcat cggcacgacc
     2341 cgacggatcg tgctccccga gatccgctgc tacaccgagc ggttcgggct cgccttctgc
     2401 cccgagcgca ccatcgaggg ccgcgcgatc ccggagatgc gctcgctccc gcagatcatc
     2461 ggcggggtcg acgccgagag cgccgaccgt gccgaggccc ttttccgccg catcaccgag
     2521 agcatcgtcc gcgtctcctc gctcgaggcc gcggagatga tcaagctcat caacaacacc
     2581 tatcgcgacc tcaccttcgc cttcgcgaac gaggtggccc tcatcgccga gcacatgggg
     2641 ctctccgcgc gggagatgat ccacgcggcc aatgtgaact atccgcggtc cggcgtggcc
     2701 cggccgggct tcgtgggcgg cccgtgcctg gagaaggacg cgctgatcct catcgacagc
     2761 ctccaccaga tcgacttcaa gccccgcgtg atcgaggagg cccgccggat caaccacgcc
     2821 ctgcccgagc acgtggcggc gcgggtgctc gagcagctgc agcagctccg cggctcg
//
LOCUS       DATMCV010001028         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_720884, whole genome shotgun sequence.
ACCESSION   DATMCV010001028 DATMCV010000000
VERSION     DATMCV010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720884"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VNU03_23610"
     CDS             complement(<1..678)
                     /locus_tag="VNU03_23610"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP77531.1"
                     /translation="MDARVSRRSFLATGLAAGGVLVVGFGLDGPGRSAAQSIPDADRF
                     LGKSLAPDMVDSMLAVQRDGSVTVFVGRVDVGTGARIALRQIVGEELDVPIERIAMIE
                     GDTALTPNQGGTGGSYGVARGGVQLRRAAATARQALLEMGAQRLGRPAGDLEVADGVV
                     RGKDGTGSVTYGELIGDRAFNLKVDAAAPLKPPDRFRFIGKSLPRPDVPEKVTGHHRY
                     LHDLTLPG"
     gene            complement(678..1154)
                     /locus_tag="VNU03_23615"
     CDS             complement(678..1154)
                     /locus_tag="VNU03_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWP77532.1"
                     /translation="MAKLTLKVNGTSREVESDDPDTPLLYVLRGDLGLTGTHFGCGLA
                     QCGACTVLVGGRAVRSCIAPARSVVGQEVVTIEGLGSPERPDPVQAAFIAEQAAQCGY
                     CTAGMVMSARALLAQTPRPTEQQVREGLAGNLCRCGSHARVIRAVLRAAAATPRAR"
     gene            complement(1219..>2321)
                     /locus_tag="VNU03_23620"
     CDS             complement(1219..>2321)
                     /locus_tag="VNU03_23620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77533.1"
                     /translation="RDAMARARETVGGATRRERQHVEALSAFVGGESARGFALADEHV
                     KEFPRDAVLVNQVSSAIALAGAADREEHRVGFMERLAPAYGDDWWYQSALAFTYHEVD
                     RFEESRRLSEASLRQHPRNAHASHNLAHIAFETLDVAAGAAFLDEWMAGYDRRASFHC
                     HLAWHQAMFALHEGRYARALDIFERDILRSGNPRSAMTDGTALLWRVKLDGASEQPLP
                     WRSLAELAAKVSRPGFLFGEVHAAFAYAACGDEAALAKMMDGLRALGAKGHPIAASLV
                     LPLVQGTAAFAAGDHAGALRLFEPVEAEMHRIGGSHAQWEIYEETMVQCYLALERYDD
                     ALRLVHRRLARRASPRDQKWLARASARGTEAR"
BASE COUNT          307 a          867 c          842 g          305 t
ORIGIN      
        1 gcccggcagc gtcaggtcat ggagataacg atggtgcccg gtgaccttct ccggcacgtc
       61 aggccgcggc agcgacttgc cgatgaaccg gaagcgatca ggcggcttca acggggccgc
      121 cgcgtccacc ttcaggttga acgcgcggtc gccgatcagc tcgccgtagg tcaccgagcc
      181 ggtgccgtcc ttgccccgca ccacgccgtc ggccacctcg aggtctccgg ccggccggcc
      241 gagccgctgc gcccccatct ccagcagcgc ctggcgcgcg gtcgcggcgg cgcggcggag
      301 ctggacgccg ccgcgggcga cgccgtagct gcccccggtc ccgccctggt tgggcgtcag
      361 cgcggtgtcg ccctcgatca tcgcgatgcg ctcgatcggg acgtcgagct cctcgccgac
      421 gatctgccgc agcgcgatgc gcgcgcccgt gccgacgtcg acccggccga cgaaaacggt
      481 gaccgagccg tcgcgctgca ccgccagcat cgagtcgacc atgtcggggg ccagcgactt
      541 gccgaggaag cggtcggcgt cgggaatgga ttgcgccgcg gaccgcccgg gcccgtcgag
      601 gccgaagccg acgaccagca ctccgcccgc cgcgaggccg gtggcgagga agctccgtcg
      661 cgagacgcgg gcgtccatca gcgcgccctc ggagtggcgg cggccgcgcg gagcaccgcc
      721 cggatcacgc gcgcgtggct gccgcagcgg cagaggttgc cggccaggcc ttcgcgcacc
      781 tgttgctcgg tcgggcgggg cgtctgggcc aggagggccc gcgcgctcat gaccatgccg
      841 gcggtgcagt acccgcactg cgcggcctgc tccgcgatga acgcggcctg caccggatcg
      901 gggcgctcgg gtgagcccag gccctcgatc gtcaccacct cctggccgac caccgagcgc
      961 gccggcgcga tgcacgagcg gactgcgcgg ccgccgacca gcacggtgca cgccccacac
     1021 tgcgcgaggc cgcagccgaa gtgggtgccg gtcagcccga gatctcccct caggacatac
     1081 agcagcgggg tgtcgggatc gtccgactcg acctcccggc tcgtgccgtt caccttgagc
     1141 gtgagcttgg ccatacttct ctcttcctcc ctctcccctc aggggagagg gccggggtga
     1201 ggggcagcgt ccaaccgctc atcgcgcctc ggtgccgcgc gcgctcgccc gagcgagcca
     1261 cttctgatcc cgcggcgagg cgcggcgcgc aagccgccgg tgtacgaggc gcagggcgtc
     1321 gtcgtagcgc tcgagcgcga ggtagcactg caccatggtc tcctcgtaga tctcccactg
     1381 ggcgtggctg ccgccgatgc ggtgcatctc ggcctcgacc ggctcgaaga ggcgcagcgc
     1441 ccccgcgtgg tcgcccgccg cgaacgcggc ggtcccctgc accagcggca gcacgaggct
     1501 ggccgcgatc gggtggccct tcgcgcccag cgcccgcagg ccgtccatca tcttcgccag
     1561 cgccgcctcg tcgccgcagg cggcgtaggc gaaggccgcg tgcacctcgc cgaagaggaa
     1621 gccgggacgc gagaccttgg cggccagctc ggccagggac cgccagggca gcggctgctc
     1681 gctcgcgccg tcgagcttca cccgccagag cagcgcggtg ccgtcggtca tggccgagcg
     1741 cgggttgccc gagcgcagga tgtcgcgctc gaagatgtcg agggccctcg cgtagcggcc
     1801 ctcgtggagc gcgaacatcg cctggtgcca ggccaggtgg caatggaacg acgcgcgccg
     1861 gtcgtagccc gccatccact cgtcgaggaa ggcggcgccc gccgcgacgt cgagggtctc
     1921 gaacgcgatg tgggccaggt tgtgcgaggc gtgcgcgtta cgtggatgct gtcgtagtga
     1981 cgcctccgag aggcgccgcg actcctcgaa gcgatccacc tcgtggtagg tgaacgcgag
     2041 cgcggactgg taccaccagt cgtcgccgta ggcgggggcg agccgctcca tgaaacccac
     2101 ccggtgctcc tcgcgatcgg cggccccggc cagggcgatg gcgctgctca cctggttcac
     2161 cagcaccgcg tcgcgcggga attccttcac gtgctcgtcc gcgagcgcga agccgcgggc
     2221 gctctcgccg cccacgaagg cggagagggc ctccacgtgc tgccgctcgc gccgggtggc
     2281 gccgcccacc gtctcgcgcg cgcgggccat ggcgtcgcgc g
//
LOCUS       DATMCV010001029         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_855206, whole genome shotgun sequence.
ACCESSION   DATMCV010001029 DATMCV010000000
VERSION     DATMCV010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855206"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VNU03_23625"
     CDS             <1..898
                     /locus_tag="VNU03_23625"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HWP77534.1"
                     /translation="RLSGSCGEHVVPITALHLHELATVRDPFEAVFVAVKSYDTEWAT
                     QLAVDYLRRPDGVVVDFQNGINDHRVAAVAGRERTLGCVILISAGMYEAAHAIRTDRG
                     SAAFKIGEHDGSDSPRARRLAAIMSDVAPAVVTTNLWGERWSKLALNCMLNPLAGLSG
                     LGTAEVRSEPLTRRLAAHLGAEVVRVGTALGHRIEPILGIAAQRFADATDSSAIEILG
                     KEIAADAASRSGGRPSMLQDVMKGRRTEIDYLNGFVAAEGRAVGIATPFNDAVVDAFH
                     AFPVGTLTAAPRNLDPLVRLLA"
     gene            914..1636
                     /locus_tag="VNU03_23630"
     CDS             914..1636
                     /locus_tag="VNU03_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWP77535.1"
                     /translation="MDLLVVLAVGLAAGTISGIVGFGSSIMLMPVLVVAYGPLHAVPI
                     MAIAAILANLSRVIIWWREVDWRAVAAYSVTGVPAAALGARTLLALPPRLIEGALGVF
                     FLGMIPARRWLARRGLGLRPRHLLVIGAVVGFLTGIVVTTGPITAPIFLSYGLVKGAF
                     IATEAAGSLAVYLSKAAVFRSFGAMPLPVIVQGLITGTSLMLGAWIAKRFVLRLHPDR
                     FRLLMDALMLLSGAAMLYTAFR"
     gene            complement(1729..>2032)
                     /locus_tag="VNU03_23635"
     CDS             complement(1729..>2032)
                     /locus_tag="VNU03_23635"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP77536.1"
                     /translation="NAARVAHKPSLLGLLDALFLTRTKGEWIDLLEAGGVPCGPIHSL
                     PEAVAQPQADAIGIIQRLPGDDYDVVSLPISFDGRRPPIRRAPPRLGEHNAEIRGQ"
BASE COUNT          246 a          734 c          736 g          316 t
ORIGIN      
        1 ccggctgtcc ggcagctgcg gcgagcacgt ggtcccgatc accgcgctcc accttcacga
       61 gctggccacg gtgcgcgacc cgttcgaggc ggtcttcgtg gcggtgaagt cctacgacac
      121 cgagtgggcc acccagctcg cggtggacta cctgcggcgg cccgacggcg tggtcgtcga
      181 tttccagaac ggcatcaacg accaccgggt ggccgcggtg gccggccgcg agcgcaccct
      241 cggctgcgtc atcctgatca gcgcgggcat gtacgaagcc gcccacgcca tccgcaccga
      301 ccgcggcagc gccgcgttca agatcggcga gcacgacggc agcgactcgc cgcgggcgcg
      361 ccggctggcc gcgatcatga gcgacgtggc gcccgcggtg gtgaccacca acctgtgggg
      421 cgagcgctgg tcgaagctcg cgctgaactg catgctgaac ccgctcgcgg ggctgagcgg
      481 cctcggcacc gccgaggtgc ggtcggagcc gctcacccgc cggctggccg cccatctggg
      541 cgccgaggtg gtgcgggtcg gcaccgcgct cgggcaccgc atcgagccca tcctgggcat
      601 cgcggcgcag cgcttcgcgg atgccaccga ctcctccgcg atcgagatcc tcggcaagga
      661 gatcgcggcg gacgcggcca gccgcagcgg tgggcggccg tcgatgctgc aggacgtgat
      721 gaaggggcgg cgcaccgaga tcgactacct caacgggttc gtggccgccg aggggcgcgc
      781 ggtcggcatc gcgaccccgt tcaacgacgc ggtggtcgac gccttccacg ccttcccggt
      841 gggcacgctc accgccgcgc cccgcaacct cgacccgctc gtccgcctgc tcgcctagcc
      901 gcggcccgcc cgcgtggacc tgctggtcgt gctggcggtc ggtctggccg ccggcaccat
      961 cagcggcatc gtcggcttcg gctcgtcgat catgctgatg ccggtgctcg tggtcgcgta
     1021 cggcccgctc cacgcggtgc cgatcatggc catcgccgcg atcctcgcca acctgtcgcg
     1081 ggtgatcatc tggtggcggg aggtggactg gcgcgcggtc gcggcctatt cggtcaccgg
     1141 cgtccccgcg gccgcgctgg gcgcgcgcac cttgctggcg ctgccgccgc ggctgatcga
     1201 gggggcgctc ggcgtgttct tcctcggcat gatcccggcg cggcgctggc tcgctcggcg
     1261 ggggctgggg ctgcggccgc ggcacttgct ggtgatcggg gcggtggtgg gcttcctcac
     1321 cggcatcgtg gtgaccaccg ggccgatcac cgcgccgatc ttcctctcgt acggcctcgt
     1381 gaagggcgcg ttcatcgcca ccgaggccgc cggctcgctc gcggtctacc tgagcaaggc
     1441 cgcggtcttc cgcagtttcg gggccatgcc gctccccgtg atcgtgcagg gcctcatcac
     1501 cggcacgtcg ctgatgctcg gggcctggat cgccaagcgc ttcgtcctgc gcctgcaccc
     1561 cgaccgcttc cgcctcctga tggacgccct gatgctcctc tcgggcgccg ccatgctcta
     1621 caccgcgttc cgctagggcg ggggtcaggt cttacattcc gacatttccg cgcattgcgt
     1681 ggccactact cgcgcgggag aaatgtcgga atgtaagacc tgaccccgct actgaccccg
     1741 aatctccgcg ttgtgctcgc cgaggcgcgg gggggcgcgg cggatgggcg ggcgccggcc
     1801 gtcgaaggag atcgggagcg agacgacgtc gtagtcgtcg ccggggaggc gctggatgat
     1861 gccgatggcg tcggcctgcg gctgggccac cgcctccggc aggctgtgga tcggcccgca
     1921 cggcacgccg cccgcttcca ggagatcgat ccactcgccc ttggtccggg tgaggaagag
     1981 ggcgtcgagc agcccgagca gcgagggctt gtgggccacg cgcgccgcgt tg
//
LOCUS       DATMCV010001030         2920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_789059, whole genome shotgun sequence.
ACCESSION   DATMCV010001030 DATMCV010000000
VERSION     DATMCV010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789059"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VNU03_23640"
     CDS             complement(<1..657)
                     /locus_tag="VNU03_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HWP77537.1"
                     /translation="MSDANGFDYPVMARALILGDKDTVARKTQEGLALAMDPKDLIFR
                     GLIPGMDVVGEKFRRNEYYVPQVLLSARAMYAGLDLLKPLITQAGGAGQNLGVVVIGT
                     AQGDLHDIGKNLVAMMLEGAGFKVINLGRDVAPEKFVAAVKEHDAQIVGISALMTTTM
                     PAMKRTIDALEKAGLRAQVKVMIGGAPVNQAYADEIGADGYAKDSTIAVVRAKELLGV
                     P"
     gene            complement(654..2036)
                     /locus_tag="VNU03_23645"
     CDS             complement(654..2036)
                     /locus_tag="VNU03_23645"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWP77538.1"
                     /translation="MIFQQLLNEEAGCLSYLIGCSQAGQGIIVDPARDRVDDYVALAR
                     RKGLTVTGIVETHVHADHISGNQALAAKTGARIHIHPAANAAFAHEPVEHGRQLRVGS
                     VALDVVHTPGHTPESISLLITDLSRGNEPWFVLTGDTLFVGDVGRPDFGGEQAAARLY
                     ASLTERLLRLPDSVEVYPAHGAGSSCGRAMSSKTATTIGFERRFNSALQASGVDDFVR
                     HLMTGLPPKPPNFDRIIARNRAQALAAGGPPRPLSAAQTCEAIDKGAFVLDVRTPEEY
                     GQGHIPGAIHVWIESPQFANRAGLFIPAEAPVVLVVSSPTDLERAVQGLGRIGLDAIA
                     GQLQWGMTEWKSQGLPMEQVPQISVHDLATMKDERPDLVVIDVREPFEWDEGHIEGAL
                     HVPMSEALARKDLVPAGRPKAVLCAGGLRSSTVISALSRAGLTNWYNVIGGMTAWQKG
                     GYSTVKRAAP"
     gene            complement(2104..>2920)
                     /locus_tag="VNU03_23650"
     CDS             complement(2104..>2920)
                     /locus_tag="VNU03_23650"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWP77539.1"
                     /translation="PSPGRTRPVTAVYVFLPLAIFLGLGFLTHLAEVRWPLRRYSKPK
                     ALRLDLIGYAVTVLARIAIEKFQVPAIERLPSLRGFAWMTEVSRIVQWRVSWPIALVV
                     SVVVLDFLLYWGHRLLHTAMLWHTHAAHHSVEHLYWFGGNRASPVHVASLAACGTLLG
                     LVWPVHGGHPGLVIELVIYACIQHFNHANLSWRLGFLGSLFVTPRYHFVHHGAEARLN
                     GSNFGFLLTIWDRMFGTYVSPAGLPNDFPLGLSYRVGAARMWIGLPPGRPSEG"
BASE COUNT          452 a         1020 c         1009 g          439 t
ORIGIN      
        1 cggcacgccc agcagctcct tggcgcgcac caccgcgatc gtggagtcct tggcgtagcc
       61 gtccgcgccg atctcgtccg cgtaggcctg gttcaccggc gcgcccccga tcatgacctt
      121 gacctgggcc cgcagcccgg ccttctcgag cgcgtcgatg gtgcgcttca tggccggcat
      181 ggtggtggtc atgagcgcgg agatccccac gatctgggcg tcgtgctcct tcaccgcggc
      241 cacgaacttc tccggcgcca cgtcccgtcc gaggttgatc accttgaagc ccgcgccctc
      301 cagcatcatc gcgaccaggt tcttgccgat gtcgtgcagg tcaccctgcg cggtgccgat
      361 gaccaccacc cccaggttct gcccggcccc tcccgcctgg gtgatgagcg gcttgagcaa
      421 gtcgagaccc gcgtacatgg cccgcgcgga caggaggacc tgcggcacgt agtactcgtt
      481 gcggcggaac ttctcgccca ccacgtccat gcccgggatg agcccgcgga agatgagatc
      541 cttcgggtcc atcgccaggg cgagaccttc ctgggtcttg cgggccaccg tgtccttgtc
      601 gcccaggatc agggcgcggg ccatcacggg atagtcgaag ccgttcgcgt cactcatgga
      661 gcggcccgct tcaccgtgct gtagcccccc ttctgccagg cggtcatccc gccgatgacg
      721 ttgtaccagt tcgtgaggcc ggcgcggctg agcgcgctga tcaccgtgct ggagcgcagc
      781 ccgcccgcgc agagcaccgc cttcgggcga ccggccggca ccaggtcctt ccgggcgagg
      841 gcctcgctca tcggcacgtg gagcgcgccc tcgatgtggc cctcgtccca ctcgaagggc
      901 tcgcgcacgt cgatcaccac caggtcgggc cgctcgtcct tcatggtggc gaggtcgtgc
      961 accgagatct ggggcacctg ctccatcggc aggccctggc tcttccactc ggtcatcccc
     1021 cactgcagct gtcccgcgat cgcgtcgagc ccgatccgcc cgagcccctg caccgcacgc
     1081 tcgaggtcgg tggggctcga caccacgagg accaccggcg cctccgcggg gatgaacagc
     1141 ccggcgcggt tcgcgaactg cgggctctcg atccacacgt ggatcgcccc ggggatgtgg
     1201 ccctgcccgt actcctcggg ggtgcgcacg tcgagcacga aggcgccctt gtcgatggcc
     1261 tcgcaggtct gcgcggccga cagcgggcgc ggcggcccgc cggcggccag ggcctgggcc
     1321 cggttgcggg cgatgatgcg gtcgaagttc ggcggcttgg gcggcaggcc ggtcatcaga
     1381 tggcgcacga agtcgtcgac cccgctcgcc tgcagggcgc tgttgaagcg gcgctcgaag
     1441 ccgatcgtgg tggcggtctt gctcgacatg gcgcggccgc agctcgagcc ggccccgtgc
     1501 gcgggataga cctccacgct gtcgggcagg cggagcaggc gctcggtgag gctcgcgtag
     1561 agccgggccg cggcctgctc gccgccaaag tccggccggc cgacgtcgcc cacgaagagc
     1621 gtgtccccgg tcagcacgaa ccacggctcg ttgccccggc tcagatcggt gatcagcagc
     1681 gagatgctct cgggggtgtg accgggcgtg tggacgacat cgagggccac gcttcccacc
     1741 cgcagctgcc ggccgtgctc gaccggctcg tgggcgaagg ccgcgttggc ggccgggtgg
     1801 atgtggatgc gtgccccggt cttggccgcc agggcctggt tacccgagat gtggtcggcg
     1861 tggacgtgcg tctcgacgat gccggtgacg gtgagcccct tgcgtcgggc cagggccacg
     1921 tagtcgtcca cccggtcccg cgccggatcg acgatgatgc cctggccggc ctgcgagcag
     1981 ccgatcaggt aggagagaca gccagcctcc tcgttcaaca gctgctgaaa gatcacgcgt
     2041 gaagtatgcc atacccggcc cccgaggcgc cacgacgacg cggagttacg ggtcgggcgc
     2101 cactcacccc tcgctcggtc ggcccggcgg cagcccgatc cacatccgag cggcgcccac
     2161 ccggtagctc agcccgagcg gaaagtcgtt cggaaggccg gccgggctca cgtaggtgcc
     2221 gaacatccga tcccagatcg tcagcaggaa gccgaagttg ctgccgttca gcctggcctc
     2281 ggctccgtga tggacgaagt ggtagcgagg ggtgacgaag agcgagccga gaaatcccag
     2341 acgccagctc agattggcgt ggttgaagtg ctgaatgcag gcatagatca ccagctcgat
     2401 gacgagaccg ggatggcctc cgtgcaccgg ccacaccagc cccaggagcg tcccgcaggc
     2461 ggccaggctg gcgacgtgaa ccggtgaggc gcggttgccg ccgaaccagt agaggtgctc
     2521 caccgagtga tgcgcggcgt gcgtgtgcca cagcatcgcg gtgtggagca ggcgatgacc
     2581 ccagtacagg agaaaatcga ggaccacgac cgacaccacg agggcgatcg gccagctcac
     2641 cctccattgc acgatgcggg agacctcggt catccaggcg aagccgcgca ggctcggcaa
     2701 tcgctcgatg gccgggacct ggaacttctc gatcgcgatc cgcgccaaga ccgtcaccgc
     2761 gtagccgatg aggtcgagcc gcagggcctt cggcttcgaa tacctccgga gcggccaccg
     2821 cacctccgcg aggtgggtca ggaaaccgag gccgagaaag atcgcgagcg gcaagaagac
     2881 gtacaccgcg gtcacggggc gagtcctccc gggggagggc
//
LOCUS       DATMCV010001031         4151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_720933, whole genome shotgun sequence.
ACCESSION   DATMCV010001031 DATMCV010000000
VERSION     DATMCV010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720933"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VNU03_23655"
     CDS             complement(<1..271)
                     /locus_tag="VNU03_23655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77540.1"
                     /translation="MSLRGVLLGAFVAGLGVSITLSESALALLALLWLWRLRDPAVRG
                     AARWPLAAPVLAFSAATVLSALLSGHPLASLDAARGLLLAAALYVT"
     gene            complement(268..1665)
                     /locus_tag="VNU03_23660"
     CDS             complement(268..1665)
                     /locus_tag="VNU03_23660"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HWP77541.1"
                     /translation="MLKAYSRLLEQAMLVGDLVLVAVCWLLAYWLRFHLPGPPLPYQN
                     VPPVSDYLLMLLPILVVWGVAFHAFGLYRPRRIGSHLSEAADVAKASTMGALVLVAVM
                     TFFFRGYDYSRVVIVYFWLLSIGLVWFSRAAFREGLRFARRRGYNLRYAVVVGGGELA
                     ATVVQRMQGRPDVGIQVLGVVSDDKDAAGGARWLGGYSDLRAVLDAHTVDHVVLALAH
                     EDYGRLVGLLDAVGDEPVTIHVVPDLLRFASLHGGVEQFEGMPFIHLRESPLYGWSSL
                     AKRVFDFVFSLAVLAALWPLLIALAVAVRFSSPGPVFYRQERMGLDGRRFRMLKFRTM
                     QADAERQTGPKWADPADERRTGLGAFLRRFSLDELPQFLNVVRGDMSVVGPRPERPVF
                     VEQFRQTVPGYMLRHKVKSGITGWAQVHGLRGNTSLEKRIQYDIEYIERWSVWLDLKI
                     IAMTVVRVLVDRNAY"
     gene            complement(1659..2795)
                     /locus_tag="VNU03_23665"
     CDS             complement(1659..2795)
                     /locus_tag="VNU03_23665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP77542.1"
                     /translation="MPDLARARVALVHDWLTGMRGGERCLEVFCELFPTADLYTLLHV
                     PGRAAPAIERRRIVTSWIQRLPQAERRYRQYLPLFPAAIGAFDLRGYDLVLSSSHAVA
                     KSVRVPPGARHVCYCFTPMRYVWDLYDDYFGPRAGLAARVLMPPVAAWLRRWDRATAA
                     RVHHFVAISRFVAERIRRVYDRDADVIYPPVDVARFRVDDAPGDFYLVVSALTPYKRV
                     DLAVEACNRLGRRLVVVGTGPEERRLRALAGPTVELLGWRDDAQTAELYARCRALLFP
                     PLEDFGITPLEAMASGRPVIAFGQGGARETVVAPGGAEAPTGLFFERQTVDDLEAAIR
                     RFESGATVFEPKALRRRAEAFDRPLFKERIQAYLETRLAEAPPC"
     gene            complement(2832..3800)
                     /locus_tag="VNU03_23670"
     CDS             complement(2832..3800)
                     /locus_tag="VNU03_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWP77543.1"
                     /translation="MRVLITGITGFVGSYMAEHALSLGAEVFGSIRWRSKTDNIDHLR
                     AKVTLIESDLRDLSSVRNLLETSNPTHVLHLAAQSFVGVSWKAPAETLTTNILSQVNL
                     LEGLRGLKMSPRFLVVGSSEEYGMVGEDELPIKETNPLRPLSPYAVSKVAQDLMGYQY
                     WKSYGLPIVRSRAFNHEGPRRGDVFVTSNFARQVAEIEAGQRDPVIYIGDLTPRRDYS
                     DVRDIVRGYWALVERGEPGEVYNLCSGKTWAIQRVLDFLLEQSQVKGISVQTDPARLR
                     PSDVMVLEGDPSKIEKATGWKVEIPFERTLKDLLGYWRQRTWSSSR"
BASE COUNT          707 a         1469 c         1459 g          516 t
ORIGIN      
        1 cggtgacgta gagcgcggcg gcgagcagca gccccctcgc ggcatccagg ctcgcgagcg
       61 gatgccccga gagcagcgcg gagagcacgg tcgccgcgct gaacgccagc accggggcgg
      121 cgagcggcca gcgggcggcc ccgcggacgg ccggatcgcg caggcgccag agccagagca
      181 gggcgagcag ggcgagcgcg gactcggaca gcgtgatcga gaccccgagc ccggcgacga
      241 aggcgccgag gagaaccccg cgaaggctca atacgcgttc cggtccacga gcacccgcac
      301 caccgtcatc gcgatgatct tcaggtcgag ccacaccgac cagcgctcga tgtactcgat
      361 gtcgtactgg atgcgcttct cgagcgaggt gttcccccgg agcccgtgca cctgggccca
      421 gccggtgatg cccgacttga ccttgtggcg cagcatgtag ccgggcaccg tctggcggaa
      481 ctgctcgacg aagaccggtc gctccggccg cgggccgacc acgctcatgt cgccccggac
      541 cacattgagg aactggggca gctcgtcgag gctgaagcgg cgcagaaaag cccccagccc
      601 ggtgcgccgc tcgtcggcgg gatcggccca cttggggccg gtctgccgct cggcgtcggc
      661 ctgcatggtg cggaacttga gcatgcggaa gcgccggccg tcgagcccca tccgctcctg
      721 ccggtagaac acggggccgg gcgaggagaa ccgcaccgcg accgccaggg cgatcagcag
      781 cggccagagc gcggcgagga ccgccagcga gaagacgaag tcgaagaccc gcttggcgag
      841 cgagctccat ccgtagagcg gcgactcgcg caggtggatg aacggcatcc cctcgaactg
      901 ctcgaccccg ccgtggagcg acgcgaagcg gaggagatcc ggcaccacgt ggatcgtcac
      961 cggctcgtcg cccaccgcgt ccaggaggcc caccaggcgg ccgtagtcct cgtgggcgag
     1021 ggccagcacc acgtggtcca ccgtgtgcgc gtcgaggacc gcacgcaggt ccgagtagcc
     1081 gccgagccag cgcgccccgc cggccgcgtc cttgtcgtcg ctcacgaccc cgagcacctg
     1141 gatccccacg tccggccgcc cctgcatgcg ctgcaccacg gtggcggcca gctccccgcc
     1201 acccaccacg accgcgtagc gcaggttgta gccgcgacgg cgcgcgaagc ggagcccctc
     1261 ccggaacgcg gcgcgggaga accagaccaa cccgatcgac agcagccaga agtacacgat
     1321 gaccacgcgc gagtagtcgt acccgcggaa gaagaaggtc atcaccgcga ccaggaccag
     1381 cgcgcccatc gtggacgcct tggccacgtc cgcggcctcg gagagatgcg acccgatgcg
     1441 ccgcggccgg tagaggccga aggcgtggaa ggccacgccc cagaccacga ggatcggcag
     1501 cagcatgagc aggtagtcgg acaccggcgg cacgttctga tacggcagcg gcggccccgg
     1561 gagatggaag cgcagccagt aggcgaggag ccagcacacc gcgaccagta cgaggtcgcc
     1621 gaccagcatg gcctgctcga gcagccgcga gtaggccttc agcacggggg ggcctccgcg
     1681 agccgcgtct ccaggtacgc ctggatccgc tccttgaaca gcgggcgatc gaaggcctcg
     1741 gcccggcggc gcagggcctt cggctcgaac acggtcgcgc cggactcgaa gcgccggatc
     1801 gccgcctcca ggtcgtcgac cgtctggcgc tcgaagaaga ggccggtggg ggcctcggct
     1861 ccgcccggcg ccaccaccgt ctcgcgggcg cccccctgtc cgaaggcgat gaccgggcgg
     1921 cccgacgcca tcgcctcgag cggggtgatg ccgaagtcct cgagcggcgg gaagagcagg
     1981 gcgcggcagc gcgcgtagag ctccgcggtc tgggcgtcgt cgcgccagcc gagcagctcc
     2041 acggtcggcc cggccagggc ccgcagccgc cgctcctccg ggccggtgcc caccaccacg
     2101 aggcgccggc cgagccgatt gcaggcctcg acggccaggt ccacgcgctt gtagggggtc
     2161 agcgccgaga ccaccaggta gaaatcgccc ggcgcgtcgt cgacgcggaa gcgggcgacg
     2221 tcgacgggcg ggtagatgac gtcggcgtcg cgatcgtaga cccgacggat gcgctcggcc
     2281 acgaagcgcg agatggccac gaagtggtgg acccgcgccg cggtcgcccg gtcccaccgg
     2341 cgcagccacg cggccacggg cggcatcagc acccgcgcgg ccagccccgc gcgcgggccg
     2401 aagtagtcgt cgtagaggtc ccacacgtag cgcatcgggg tgaagcagta gcacacgtgc
     2461 cgggcgcccg gcgggacgcg cacgctcttg gcgaccgcgt ggctcgagga gagcaccagg
     2521 tcgtagccgc gcaggtcgaa ggccccgatc gcggccggga agagcggcag gtactgccgg
     2581 tagcgccgct cggcctgagg caggcgctgg atccacgagg tcacgattcg gcgtcgctcg
     2641 atcgcaggcg ccgccctacc cgggacgtgc agcaaggtat agagatcggc ggtggggaag
     2701 agctcgcaga agacctcgag acagcgctcg ccgccacgca tgccggtgag ccagtcgtgg
     2761 acgagggcga cccgggcgcg cgctagatcc ggcacgacgg tgcgaccccg tccatcacgg
     2821 ctcagcccac gtcagcggga cgacgaccag gtccgctgtc gccagtatcc cagcagatcc
     2881 ttgagggtgc gctcgaaggg aatctccacc ttccagccgg tcgccttctc gatcttggac
     2941 ggatcgccct ccagcaccat gacgtccgag ggccgcaggc gcgccggatc cgtctgcacg
     3001 ctgatcccct tcacctgcga ctgctcgagc aggaagtcga gcacccgctg gatggcccac
     3061 gtcttcccgg agcagaggtt gtagacctcg cccggctcgc cgcgctccac cagcgcccag
     3121 tacccgcgca cgatgtcgcg cacgtcggag tagtcgcggc gaggcgtcag gtcgccgata
     3181 tagatcaccg gatcgcgctg gcccgcctcg atctcggcca cctgccgggc gaagttcgag
     3241 gtgacgaaca cgtcgccccg gcgcgggccc tcgtggttga acgcccggct ccgcacgatg
     3301 ggcaggccgt agctcttcca gtactggtag cccatcaggt cctgggcgac cttgctcacc
     3361 gcatacgggc tgagcggccg gagcggattc gtctccttga tcggcagctc gtcctcgccc
     3421 accatcccgt attcctcgct gctgcccacc accaggaagc gcggcgacat cttgagccct
     3481 cgcaggcctt cgagcaggtt cacctgcgag aggatgttgg tggtgagggt ctccgccggc
     3541 gccttccagg agacccccac gaagctctgg gccgccaggt gcaggacgtg ggtcggattc
     3601 gaggtctcga gcaggttccg caccgaggag aggtcgcgga ggtcggactc gatcagggta
     3661 acctttgccc ggagatggtc gatgttgtcg gtcttgctgc gccaccggat cgagccgaag
     3721 acctccgccc ccagcgagag ggcgtgctcg gccatgtagc tgccgacgaa cccggtgatg
     3781 ccggtgatca ggacgcgcat cgccaagatt atgaaacagc gccgacggga aggctagcta
     3841 aatgcgcgcc ccatcaggcc aagtcgcccc ggaggccggt gaaaccagag gcggatcggt
     3901 cgggctcatg gccctccgga ggatcccggc caggagctgt ccgaccagaa ttacaggacg
     3961 cggatgcact tacccgacca gctggacgag atcgtaggag tagtcggtga agggcttcgt
     4021 ccacttcgtg gccttcacga tcgcgccgag cagccagccc gtgccgagac tatacgcccc
     4081 gcctcgccgc ggcgcggtag aggtcggcga tctccgcgac catccgtgcg tggtcgaagc
     4141 gctgcgcgac c
//
LOCUS       DATMCV010001032         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_190820, whole genome shotgun sequence.
ACCESSION   DATMCV010001032 DATMCV010000000
VERSION     DATMCV010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190820"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VNU03_23675"
     CDS             complement(<1..638)
                     /locus_tag="VNU03_23675"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWP77544.1"
                     /translation="MPKSSPDSPPPPPPLIRYHAEGPVTTEYRVGLCAWQDKSMVEEG
                     SFYPIKSMKAEERLWWYSRFFDCVEVNSTFYAPLSAQNAVLWAKRTPPGFLFSVKAYA
                     LLTGHHLDAGRLPPPLLGLLPARARPDADGRLDNAVFGEEAREWAFAAFREALRPLAD
                     AGKLGYVLFQMAPWVKYGDRAVEYLASLPARLPGVTIAVEFRDASWLPRHAE"
     gene            complement(616..>2251)
                     /locus_tag="VNU03_23680"
     CDS             complement(616..>2251)
                     /locus_tag="VNU03_23680"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWP77545.1"
                     /translation="ALSMAGAIAGTLLMARYTSVSVYAPNIVTMIGLGVAIDYSLFIV
                     SRFREEIRGHPEPEALARTMATAGRAILFSGVTVAIGLLGMVFLGLGNIGSIGWAGTV
                     VVSLAVLYALTLLPALLAVIGPRVNSLRLPFIHPERSRKGRGLWHRVAVVVMTHPWLV
                     FLSVSALLLVLGLPFLHLQVGSGDVNALPPEAVSRRGDEALREHFPGGDSNRVLVVVR
                     YPDGSPLTAERIGGLYDLSRWLAKRPNVARVESLVDLDPRVTREQYQQLATAPPEMRP
                     PGVQQLLRQTVGGRLALLVVATPLKPSSPEARALVEEIRASHPAIGGEVLVTGQTAFD
                     LDIINLVTRHAPLTVGLVMLATYVVLFLLLGSVLLPLKAVIMNLLSISASYGALVWIF
                     QEGHLSGWLNFTPGPIQTATPLIMFCFVFGLSMDYEVLLLSRVREEYERGGDTTHAVA
                     EALESTGRLITGAAAIMAAVFFGFATARSVIIQAVGIGIGLAVVIDATIVRALLVPAT
                     MRLMGRWNWWAPGPLASLHRRLGLAERSSNAEVVPR"
BASE COUNT          368 a          777 c          842 g          264 t
ORIGIN      
        1 tcctcggcgt ggcgcggcag ccacgaggcg tcgcggaact ccaccgcgat ggtcacgccg
       61 ggcagccgcg cgggcagcga ggcgaggtac tccaccgccc ggtctccgta cttcacccag
      121 ggcgccatct ggaagaggac gtagccgagc ttgcccgcgt ccgcgagcgg ccggagggcc
      181 tcgcgaaacg cggcgaacgc ccactcccgc gcctcctcgc cgaagaccgc gttgtcgagg
      241 cggccgtccg cgtccggtcg ggcgcgggcg ggcagcaggc cgagcagcgg cggcggcagg
      301 cggccggcat ccaggtgatg cccggtgagg agcgcgtagg ccttgacgct gaagaggaac
      361 ccgggcggcg tgcgcttggc ccagagcacc gcgttctggg ccgagagcgg cgcgtagaag
      421 gtggagttga cctcgacgca gtcgaagaag cggctgtacc accagagccg ctcctccgcc
      481 ttcatgctct tgatgggata gaacgagccc tcctccacca tgctcttgtc ctgccaggcg
      541 cagaggccca cccggtactc ggtggtgacc gggccctcgg cgtgataccg gatcagcggg
      601 ggcggcggag gcgggctatc gggggacgac ttcggcattg gagctgcgct cggccagccc
      661 caggcggcgg tggagcgacg ccagcggccc gggcgcccac cagttccagc gtcccatcag
      721 gcgcatggtg gcgggaacca gcagcgcgcg cacgatggtg gcgtcgatca ccaccgccaa
      781 gccgatgccg atgcccaccg cctggatgat gaccgagcgg gcggtggcga acccgaagaa
      841 caccgcggcc atgatcgcgg ccgcgccggt gatgaggcgg ccggtcgact cgagcgcctc
      901 cgccaccgcg tgcgtggtat cgccgccgcg ctcgtactcc tcccgcacgc ggctcaggag
      961 caggacctcg tagtccatcg agagcccgaa cacgaagcag aacatgatca gcggggtcgc
     1021 ggtctggatg gggccggggg tgaagttgag ccagcccgag aggtggccct cctggaagat
     1081 ccacaccagc gccccgtagg aggcgctgat ggacaggagg ttcatgatca ccgccttgag
     1141 cggcagcagc accgagccca gcagcaggaa cagcacgacg taggtggcga gcatgaccag
     1201 gcccaccgtg agcggcgcgt gccgggtgac caggttgatg atgtcgaggt cgaacgcggt
     1261 ctgcccggtc accagcacct cgcccccgat cgcgggatgg gaggcgcgga tctcctcgac
     1321 cagcgcgcgc gcctccgggc tcgacggctt gagcggggtc gcgaccacga gcagggcgag
     1381 ccggccgccg acggtctggc gcagcagctg ctgcaccccg ggcggccgca tctccggcgg
     1441 ggcggtggcg agctgctggt actgctcgcg ggtcacgcgc gggtcgaggt cgaccaggct
     1501 ctcgacgcgc gccacgttgg gccgcttggc cagccagcgg ctcaggtcgt agaggccgcc
     1561 gatgcgctcc gcggtgagcg gcgagccgtc cggatagcgc accaccacca ggacgcggtt
     1621 gctgtcgccc ccggggaagt gctcgcggag cgcctcgtcg ccgcgccgcg agaccgcctc
     1681 cggcgggagc gcgttgacgt cgcccgagcc cacctggagg tggaggaacg gcaggccgag
     1741 cacgagaagc agggcggaga ccgagaggaa caccagccac ggatgcgtca tcaccaccac
     1801 cgccacccgg tgccagagcc cgcggccctt gcgcgagcgc tccgggtgga tgaacggcag
     1861 ccgcagcgag ttgacgcggg gcccgatgac cgcgagcagg gccggcagca gggtcagcgc
     1921 gtagagcacc gcgagcgaga ccaccaccgt gccggcccag ccgatcgagc cgatgttgcc
     1981 gaggccgagg aagaccatgc cgaggagccc gatggcgacg gtgacgccgg agaacaggat
     2041 cgcccggccc gcggtggcca tggtgcgcgc gagggcctcc ggctccgggt ggccgcggat
     2101 ctcctcccgg aaccggctca cgatgaacag cgagtagtcg atcgcgacgc cgagcccgat
     2161 catggtcacg atgttgggcg cgtacaccga gaccgaggtg tagcgggcca tcagcagggt
     2221 gcccgcgatc gcgcccgcca tcgacagcgc g
//
LOCUS       DATMCV010001033         2250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_190837, whole genome shotgun sequence.
ACCESSION   DATMCV010001033 DATMCV010000000
VERSION     DATMCV010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190837"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VNU03_23685"
     CDS             complement(<1..813)
                     /locus_tag="VNU03_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77546.1"
                     /translation="MPAGHRAVSVLYQALLTGLVLTLVTRRGEDQAAEFVFRLFRRQH
                     LEKFLPGLAKLGLDRLPHAVACAQYHYLSNQVGGVKTEYARESDRKAWVRYPPPRWIW
                     CGASICAIPSRVNAAMLHGWHGHNGVTLGNPRLGFVCTGQTVDGDPGLEGYYLEHDHD
                     LAPDERVRFARGERMPAFDPAAAPRLDPVAWPPERLDTVVARYAMEYVRTTLPVMLAL
                     LGPDDTRALLGHAARLIGMQYHDETARLLEIEGRGPEPFARYLAALAGAQGEA"
     gene            complement(817..2088)
                     /locus_tag="VNU03_23690"
     CDS             complement(817..2088)
                     /locus_tag="VNU03_23690"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWP77547.1"
                     /translation="MKRSLTCATLLGWILTASLAFAQGLPTASPEAVGLSSARLARVT
                     ELVKGEIAKGRYPGAVALVARRGKVAYFEALGQRDPQSGAPMTKDAIFRLYSMTKPFT
                     SVAVMTLVEDGKILLNDPVSKYLPKLKGLQVSVPRVDPQTGRVSYALVPAEREITIQD
                     LLRHTSGLVYGVFTSHTGVKEAYAKEGVDWENVTPAEQIERLARAPLAHQPGSAWEYS
                     LSTDVLGRVVESVSGATLGQFLQERLFASLKMTDTAFLVPNGKAARLAQPFAKDPVSG
                     DAVKLLDVTVAQKNDAGGAGSAGTVADYARFSQMLLNGGQLDRVRILGRATVAQMSSD
                     HLGDIRVASPILARGYSFGLGFAVRKETGLNWVTGSAGEYRWGGAAGTAFWVDPKEQM
                     IVVWMTQAQPGPRRGEDRDLFRQLVQAALVD"
BASE COUNT          319 a          758 c          857 g          316 t
ORIGIN      
        1 tgcctcgccc tgcgctcccg cgagcgcggc caggtagcgc gcgaacggtt cgggcccgcg
       61 gccctcgatc tcgaggagcc gagccgtctc gtcgtggtac tgcatgccga tgagccgggc
      121 cgcgtgaccg aggagcgcgc gggtgtcgtc gggcccgagc agcgcgagca tgaccggcag
      181 cgtggtccga acgtactcca tcgcgtaccg ggcgaccacg gtgtcgagcc gctcgggagg
      241 ccacgccacc gggtcgagcc gcggcgcggc ggcggggtcg aacgcgggca tgcgctcgcc
      301 gcgcgcgaag cgcacgcgct cgtcgggggc gaggtcgtgg tcgtgctcca ggtagtagcc
      361 ctcgagcccg gggtcgccgt ccaccgtctg accggtgcag acgaagccca ggcgcggatt
      421 gccgagggtg accccgttgt ggccgtgcca gccgtggagc atggccgcgt tcacccggct
      481 cgggatcgcg cagatcgacg cgccgcacca gatccagcgg ggtggcgggt agcggaccca
      541 cgccttgcgg tcggactcgc gcgcgtactc ggtcttgacc ccgccgacct ggttggagag
      601 gtagtgatac tgcgcgcagg cgaccgcgtg gggcaggcgg tcgaggccga gcttggcgag
      661 cccggggagg aacttctcga ggtgctggcg gcggaagagc cggaagacga actccgcggc
      721 ctggtcctcg ccccgtcgcg tgaccagggt gaggaccagg ccggtcagga gcgcctggta
      781 gaggaccgag acggcccggt ggccggcggg caacggtcag tcgacgagcg ccgcctgcac
      841 gagctggcgg aagagatcgc ggtcctcacc gcgccgcggc ccgggctgcg cctgggtcat
      901 ccacaccacg atcatctgct ccttcgggtc gacccagaag gcggtgccgg cggcgccgcc
      961 ccagcggtat tcgcccgccg agccggtgac ccagttgagg cccgtctcct tgcgcaccgc
     1021 gaagccgagg ccgaagctgt agccgcgcgc caggatcggg gaggcgaccc ggatgtcgcc
     1081 caggtggtcg gaggacatct gggccacggt ggcgcggccg aggatgcgga cgcggtcgag
     1141 ctggccgccg ttcagcagca tctgcgagaa gcgtgcgtag tcggcgaccg tccccgcgct
     1201 tcccgcgccg cccgcgtcgt tcttctgggc gaccgtcacg tcgaggagct tcaccgcgtc
     1261 accgctcacc gggtccttcg cgaagggctg cgcgaggcgc gccgccttgc cgttcggcac
     1321 caggaacgcg gtgtcggtca tcttgagcga ggcgaagagg cgctcctgga ggaactggcc
     1381 gagggtggcg ccgctcaccg attcgaccac gcgacccagc acgtcggtcg agaggctgta
     1441 ctcccacgcc gagcccggct ggtgcgccag aggcgctcgc gccaggcgct cgatctgctc
     1501 ggccggcgtc acgttctccc agtcgacgcc ttccttcgcg tacgcctcct tcaccccggt
     1561 gtggctcgtg aagacgccgt agacgaggcc cgacgtgtga cgcaggagat cctggatggt
     1621 gatctcgcgc tcggccggca ccagcgcgta ggacacccgg ccggtctggg gatccacgcg
     1681 cgggacgctc acctggaggc ccttgagctt cgggaggtac ttggagaccg ggtcgttgag
     1741 caggatcttg ccgtcctcga ccagggtcat caccgcgacc gaggtgaagg gcttcgtcat
     1801 cgagtagagg cggaagatgg cgtccttggt catcggcgcg ccggactgcg gatcgcgctg
     1861 gccgagcgcc tcgaagtagg cgaccttccc acggcgggcc acgagcgcca ccgcgccggg
     1921 gtaccggccc ttggcgatct cgcccttcac cagctcggtc acgcgcgcga ggcgcgccga
     1981 ggagaggccg accgcctcgg ggctggcggt gggcaggccc tgggcgaagg cgagggaggc
     2041 ggtgaggatc cagccgagca gcgtggcgca cgtcagcgag cgcttcatgg tcgacctcct
     2101 gggcatgtga agggtcaccg ggtcactgcg ccttgaggcg gatgtcctcg taggccggga
     2161 aggcgaagac ggggatcgcg ttgatggtgt gctcggccac gcggggtccg acgccgatga
     2221 ggccgcgcag gtccatgatc ggggcgaaca
//
LOCUS       DATMCV010001034         5273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_522526, whole genome shotgun sequence.
ACCESSION   DATMCV010001034 DATMCV010000000
VERSION     DATMCV010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522526"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="nrfD"
                     /locus_tag="VNU03_23695"
     CDS             complement(<1..767)
                     /gene="nrfD"
                     /locus_tag="VNU03_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWP77548.1"
                     /translation="MSTLPAPNAAATQATWADVNRDVIRTIGMPGDRYFAWMCLVALT
                     LACGVAAWTWQIWVGIGAAGKRTPQMWAMYITTFVFWIGIGHAGTLISAVLYLFRARW
                     RTAIYRGAEAMTVFAVMTAGLFPLIHAGRMWFAYWLMPYPNQRYLWPNFKSPLVWDVF
                     AISTYLTISVVFFIVGLVPDIAALRDNATGTKRKIYAVLSLGWEGSDHQWRHYRRAYG
                     LLAALATPLVLSVHSVVSFDFAMALVPGWHSTLFAPF"
     gene            complement(764..3793)
                     /locus_tag="VNU03_23700"
     CDS             complement(764..3793)
                     /locus_tag="VNU03_23700"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP77549.1"
                     /translation="MSVMDRRSFFKIVATTGAAAAAGGCGPSAEQFIPYVIPPDNIVP
                     GIPAYFSTVCRECPSGCGLIAKNRDGRVIKLEGNPDHPTNTGSLCIRAHAGLQGLYHP
                     DRFRGPLAAGKPVTWDEAEKQLTDKLGALAKARQGARIAVVSGLETGSLGRLMDEWTR
                     LLGARPRIAYEPLGYESIRAANRVAFGRDAIPDYAIGDATYLVSFGADFLETWLNCEG
                     YTADFARMHAFYQGKQGTFVHVEPRLSLTASNADQWLRNAPGTESLLALAMLRVIVDD
                     GLHASGADLNILRAAARAGDVEGAATASGIPAETIKQVARDLARSRGGLVIGGGMAST
                     GSTSTDTLVAVNLLNAAIGAVGTRVRFGSALPLGKASPYSDVMALTQAMAAGQIDVLV
                     LVDVNPVYAMPPKSGFTEALAKVPLVVALAGRPTETTARAHVVLPILHSLESWGDYVS
                     EEGVLGLMQPTMGPVQIERKAVSALSTGDVFLRAGRNALGLEEGKGPLKWATFGDYLK
                     DEWQRVARDYGSTAPFGEFWEAALRRGGVWRQAAAPAVTLRPEAGRVQAGAAKLQGDG
                     THALIVYPSGRFYDGRGADLPWLQEVPDTMTQVSWDSWVEIPSETATKLGVKRGDLVK
                     LTSPHGSIELPAYPTDLVHPGAVAVAMGQGHKFPGTFANQGNAATGTVDQAGYLNAGA
                     NPMELLPGTPDPVSGGLPHLAVKVTLAATGGRRPLAIPQAQFDQDDREIAQWVGLGAA
                     RETELRGKAPEHASHPTMYPRVKYPEYRWGMTVDVDRCTGCQACVLACQSENNVPTVG
                     KAQVAYGRIQHWMRVERWQEGTAAKPLNMFLPMFCQHCEVAPCEPVCPVYAAYHTKEG
                     LNGQVYNRCVGTRYCGNNCPYHVRKFNWFNYTWVAPLDLQLNPDVTVRQLGVMEKCTM
                     CVQRTVAAKDLAREAGREVKDGDMQTACQQTCPTQAITFGNLKDASALVSRLALSTRG
                     YHVLEELGTTPGVTYLKKVVRSEPAGSHGKPEGGHKA"
     gene            complement(3931..5223)
                     /gene="hemL"
                     /locus_tag="VNU03_23705"
     CDS             complement(3931..5223)
                     /gene="hemL"
                     /locus_tag="VNU03_23705"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HWP77550.1"
                     /translation="MTGSDALFERAQGLMPGGVNSPVRAFRGVGGTPFFVARAEGAHL
                     VDVDGRSYVDFLGSWGPLILGHAPGPVVEAVSEAVRRGTSYGAPTGGEVEMAELIRRA
                     VPSMEMMRLVSSGTEASMSAIRLARGATGRDAIIKFDGCYHGHADSLLVKAGSGGATF
                     GVPDSLGVPRALAALTLTVPFNDLEAVRRQLEAHPAEVAAIIVEPVAGNMGVVPPAPG
                     FLAGLRELATRHGTLLIFDEVITGFRVAWGGAQARYDVRPDLTCLGKIIGGGLPVGAY
                     GGSRALMERVAPLGGVYQAGTLSGNPLAVAAGLATLRALDRPGTYERLEGLGARVERD
                     VTEAAAASGVPVTINRVGSMLTVFFTDGPVTDYASAKRADPARYARYFHAMLEGGVFL
                     AASQFEAAFVSLAHGDGDLAAAARACRSAMEKASAASK"
BASE COUNT          759 a         1886 c         1890 g          738 t
ORIGIN      
        1 aagaacgggg cgaagagcgt ggagtgccag ccgggcacca gggccatcgc gaagtcgaac
       61 gacaccacgc tgtgcaccga gagcaccagc ggggtggcca gcgcggcgag caggccgtag
      121 gcgcgccggt agtgacgcca ctggtggtcg gagccctccc acccgagcga cagcaccgcg
      181 tagatcttcc gcttggtccc ggtcgcgttg tcgcgcagag ccgcgatgtc cgggaccagc
      241 cccacgatga agaacaccac gctgatggtc aggtaggtgg agatcgcgaa cacgtcccac
      301 accagcggcg acttgaagtt tggccagagg tagcgctggt tgggatacgg catcagccag
      361 taggcgaacc acatccggcc cgcgtggatc agcgggaaga gccccgcggt catgaccgcg
      421 aagacggtca tcgcctccgc cccgcggtag atcgcggtgc gccacctcgc ccggaacagg
      481 tagaggaccg cggagatcag cgtccccgcg tggccgatac cgatccagaa cacgaaggtg
      541 gtgatgtaca tcgcccacat ctggggcgtc cgcttgccgg ccgccccgat gccgacccag
      601 atctgccagg tccacgccgc cacgccgcac gcgagcgtga gcgccaccag gcacatccac
      661 gcgaagtacc ggtcgcccgg catcccgatg gtgcggatga cgtcgcggtt gacgtcggcc
      721 caggtggcct gcgtcgcggc cgcgttcggg gcgggcagcg tgctcatgcc ttgtggcccc
      781 cctcgggctt gccgtgcgag cccgcgggct cgctccggac gaccttcttg aggtaggtga
      841 cgcccggggt ggtccccagc tcctcgagca cgtggtagcc ccgggtggag agcgccagcc
      901 gcgacaccag cgcgctcgcg tccttgaggt tgccgaaggt gatggcctgg gtcgggcagg
      961 tctgctgaca cgcggtctgc atgtcgccgt ccttcacctc gcgccccgcc tcgcgggcca
     1021 ggtccttggc cgccaccgtc cgctgcacgc acatggtgca cttctccatc accccgagct
     1081 ggcgcaccgt gacgtccgga ttgagctgca ggtcgagcgg cgccacccac gtgtagttga
     1141 accagttgaa cttccgcacg tggtaggggc agttgttgcc gcagtagcgc gtgcccacgc
     1201 agcggttgta gacctgcccg ttgaggcctt ccttggtatg gtacgcggcg tagactgggc
     1261 agaccggctc gcacggggcg acctcgcaat gctggcagaa catgggcagg aacatgttga
     1321 gcggcttggc cgcggtaccc tcctgccagc gctccacccg catccagtgc tggatgcggc
     1381 cgtaggccac ctgcgccttc ccgaccgtgg gcacgttgtt ctcgctctgg caggccagca
     1441 cgcaggcctg gcagccggtg cagcgatcca cgtccaccgt catgccccag cggtactccg
     1501 ggtacttgac ccgcgggtac atggtcgggt ggctcgcgtg ctccggcgcc ttgccgcgca
     1561 gctccgtctc gcgcgcggct ccgaggccga cccactgggc gatctcgcga tcgtcctgat
     1621 cgaactgggc ctgcgggatg gcgagaggcc gccggccgcc ggtcgcggcg agcgtgacct
     1681 tgaccgccag gtgcggcagg ccacccgaga ccggatccgg cgtgccgggc agcagctcca
     1741 tcgggttggc gcccgcgttg aggtacccgg cctgatccac ggtgccggtg gccgcgttgc
     1801 cctggttcgc gaaggtgccg gggaacttgt gcccctgccc catcgccacc gcgaccgcgc
     1861 ccggatgcac gaggtcggtc gggtaggccg gcagctcgat cgacccgtgc ggagatgtca
     1921 gcttgaccag gtcgccccgc ttgacgccca gcttggtcgc ggtctcgctc gggatctcga
     1981 cccacgagtc ccacgacacc tgggtcatgg tgtccggcac ctcctgcagc cacgggagat
     2041 cggcgccgcg gccgtcgtag aagcggccgg acgggtagac gatcagcgcg tgcgtgccgt
     2101 cgccctggag cttggccgcg ccggcctgga cgcggcccgc ctcgggacgc agcgtgaccg
     2161 cgggcgcggc cgcctgacgc cacaccccgc cgcgccgaag cgccgcttcc cagaactcgc
     2221 cgaatggagc ggtgctgccg tagtcgcggg ccacccgctg ccactcgtcc ttcaggtagt
     2281 cgccgaacgt ggcccacttg agcgggccct tgccttcctc gagcccgagc gcattccgcc
     2341 cggcgcggag gaagacgtcg ccggtggaga gcgcggagac ggccttgcgc tcgatctgca
     2401 ccggacccat ggtcggctgc atgagaccga gcacgccttc ctcggacacg tagtcgcccc
     2461 aggactcgag cgagtgcagg atcggcagca ccacgtgggc gcgcgcggtg gtctcggtcg
     2521 gccggcccgc gagggccacc acgagcggga ccttggcgag cgcctccgtg aagcccgact
     2581 tgggcggcat cgcgtacacc gggttgacgt cgaccagcac cagcacgtcg atctggccgg
     2641 ccgccatcgc ctgggtcagc gccatcacgt ccgagtacgg gctcgccttg ccgagcggca
     2701 gggcggagcc gaagcgcacg cgcgttccca ccgcgccgat ggccgcgttg agcaggttga
     2761 ccgccaccag cgtgtcggtc gacgtcgacc cggtcgaggc catgcctccg ccgatgacga
     2821 ggccaccgcg ggaccgcgcg agatcgcgcg ccacctgctt gatggtctcg gccggaatcc
     2881 cggaggcggt cgccgcgcct tccacgtcac ccgcgcgcgc ggccgcgcgc aggatgttga
     2941 gatcggcgcc cgaggcgtgg aggccgtcgt cgacgatcac ccggagcatg gccagcgcca
     3001 gcaggctctc ggtgccgggg gcgttgcgca gccactggtc ggcgttcgag gcggtgagcg
     3061 agagccgggg ctcgacgtgg acgaacgtcc cctgcttgcc ctggtagaag gcgtgcatcc
     3121 gcgcgaagtc cgcggtgtag ccctcgcagt tgagccacgt ctcgaggaag tcggcgccga
     3181 acgagaccag gtaggtggcg tcgccgatcg cgtagtccgg gatcgcgtcg cgaccgaacg
     3241 ccacccggtt ggcggcccgg atcgactcgt agccgagcgg ctcgtaggcg atgcggggac
     3301 gcgcgccgag caggcgcgtc cactcgtcca tcaggcggcc gaggctgccg gtctcgaggc
     3361 cgctgaccac cgcgatgcgc gcgccctgcc gcgccttcgc gagcgcgccc agcttgtcgg
     3421 tgagctgctt ctccgcctcg tcccacgtga ccggcttgcc cgccgcgagc gggccgcgga
     3481 agcggtccgg gtggtagaga ccctggaggc ccgcgtgggc ccggatgcag agactgcccg
     3541 tgttggtcgg gtgatccggg ttgccctcga gcttgatgac acggccgtcc cgattcttgg
     3601 cgatgaggcc gcagcccgac gggcactcgc ggcagacggt ggagaagtac gccgggatgc
     3661 ccggcacgat gttgtcgggc gggatcacgt aggggatgaa ctgctcggcc gacggcccgc
     3721 agccgccggc ggccgcagcg gcgcccgtgg tggccacgat cttgaagaac gaccggcgat
     3781 ccatcacgct catccactcc tccgcagccc agatgctgga cgttcttcgc taccggcgag
     3841 gcgcgctgtg gatgaagacc ggcggtcgac cgcgtgtttc cgggctccag ccttgtgaac
     3901 cctatcacgt ggaaaaagcg agtgtcaata ctacttcgac gctgcgctcg ccttctccat
     3961 cgcgctccga caggcccggg ccgccgcggc gaggtcgccg tcgccgtgag ccagcgagac
     4021 gaaggcggcc tcgaactgcg aggcggccag gaagacgccg ccctccagca tcgcgtggaa
     4081 gtagcgcgcg tagcgggcgg ggtccgcgcg cttggcggag gcgtagtcgg tgaccgggcc
     4141 gtcggtgaag aagacggtga gcatcgagcc cacccgattg atcgtcaccg gcacgccgct
     4201 cgcggcggcg gcctcggtca cgtcgcgctc gacgcgcgcc cccagcccct cgagccgctc
     4261 gtaggtgccg gggcgatcga gcgcgcgcag ggtggcgagc ccggcggcca ccgcgagcgg
     4321 gttgccggag agcgtgcccg cctggtagac gcccccgagc ggggccacgc gctccatgag
     4381 cgcgcgcgag cccccgtagg cgccgaccgg cagcccgccg ccgatgatct tgccgaggca
     4441 cgtgaggtcg ggccgcacgt cgtagcgggc ctgcgcgccg ccccacgcca cccggaagcc
     4501 ggtgatcacc tcgtcgaaga tcaggagggt gccgtgacga gtggccagct cgcgcagccc
     4561 ggccaggaag ccgggcgcgg gcgggaccac gcccatgttg cccgccaccg gctccacgat
     4621 gatcgccgcg acctcggccg ggtgcgcctc gagctggcgc cgcaccgcct ccaggtcgtt
     4681 gaacggcacc gtgagggtga gcgcggccag cgcgcggggc accccgaggc tgtccggcac
     4741 cccgaaggtg gcgccgcccg agcccgcctt cacgagcagg ctgtcggcgt ggccgtggta
     4801 gcagccgtcg aacttgatga tcgcgtcgcg tccggtggcc ccgcgcgcga gccggatggc
     4861 gctcatcgag gcctcggtgc ccgaggagac gagccgcatc atctccatgc tcggcaccgc
     4921 gcggcggatc agctcggcca tctccacctc gcccccggtg ggggcgccgt agctggtgcc
     4981 gcgccgcacc gcctcgctca ccgcctccac caccgggccc ggggcgtgac cgaggatgag
     5041 cggaccccac gagccgagga agtcgacgta ggagcgcccg tcgacgtcga cgaggtgggc
     5101 cccctcggcc cgcgccacga agaacggggt gccgcccact ccccggaacg cgcgcaccgg
     5161 gctgttgact ccgccgggca tcagcccctg ggcccgctcg aagagcgcgt cggagccggt
     5221 catgcggtca gggcttcttg agctcggtcg gcacgaagtc gcgcgcgtcg gaa
//
LOCUS       DATMCV010001035        16617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_461381, whole genome shotgun sequence.
ACCESSION   DATMCV010001035 DATMCV010000000
VERSION     DATMCV010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 16617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16617
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461381"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..142
                     /locus_tag="VNU03_23710"
     CDS             11..142
                     /locus_tag="VNU03_23710"
                     /inference="COORDINATES: protein motif:HMM:NF014676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HWP77551.1"
                     /translation="MRNLMQDLRATGTLIDGPAPFGPRERRLFADHLDHLLTRRRPA"
     gene            complement(162..8930)
                     /locus_tag="VNU03_23715"
     CDS             complement(162..8930)
                     /locus_tag="VNU03_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWP77552.1"
                     /translation="MPARDPLFAAPSSPFTPGTQEPIRAELFSPERLEEHAESIARQR
                     VLPRERAGRRLSPRVRDSGRVLLLCYREIASVIRDEGATTPAAEWFVDNFHVVDAVVR
                     QVREDLPPGFYRQLPKLAESPLQGYPRVLGLAWAFVAHTDSRLEPETLQRFVRAFQRV
                     QPLTIGELWAVPIALRIVLVENLRRLAERIVAGRAARQAADALANDLLGLGGRPGRPL
                     AFQGLEAAALSPAFTVELVQRLRDQDPQTTPALRWLDEQLAARGQSPDELVHTEHQNQ
                     AAMNVTVRNVITSMRMMSTLDWADFFESVSLVDDTLRGGSSFGAMDFATRDQYRHAIE
                     DLARASARSELEVTRLAMAAAKRAVSAAQRSGASPGGRAEDPGYYLISDGRRVLEKEI
                     GYHVPVRRWLLRAFLTSATPRYLATIVAVTGALLALPLLIGHASGMALGGLILLTLFA
                     VIPASDLAVALVNRAVMAVLGPLSLPRLELRDGVPPGLRTLVVVPTLLTSEADIEEQV
                     GRLEIHFLANPAGDLHFALLSDWVDAPTEAMPEDDRLLAAARDGIARLNARNGPAPPA
                     GSRFLLLHRKRLWNAGEAKWMGWERKRGKLDELNHLLAGASDTTFTATHGRAPHVPSG
                     VRYVITLDADTRLPRGAATRLVGTMAHPLNRPAIDERTGRVVDGYAVLQPRVTATIPT
                     ERGTSLFQRIFAGPAGIDPYASATSDVYQDLLGEGSYTGKGIYDVAAFTAALEGRVPE
                     NSLLSHDLFEGIFARAGLVTDIELFEEFPTDYEVAAGRQYRWARGDWQLLPWIIKGDV
                     GGGERGGGLPLIGAWKIVDNLRRTLSPPATWLTLVAAWALPGSSALVWTAFILATLGL
                     PALLPALTEVIPGRSGVSKRTHIRSVGRSFALASTQIALGITFLAHQAWLMADAIART
                     LVRLYLTRRRLLEWTTAAQAKSDLARDITSVYRRMQVPVILAVAAGALVALLRPEHAA
                     IAAPFILAWALSPLAARWVSRAPRPSTTQILSLSERQILRSTARRTWRFFETFVGPDD
                     AFLPPDNFQEDPKPTVAHRTSPTNIGLYLLAVVSAHDFGWLGILDTVDRLEATLATLH
                     RLDRFRGHFYNWYDTGGGLRPLEPRYVSTVDSGNLAAHLLTLGNACRAAIDAPLRDPG
                     RFGGLRDAVLLVQEAASVLADDRRTQTVPRGHLAETCAVLIAALHEPPADPAEWSGRV
                     RHLALHADTLVDMSRALTAERGDPVDSDVQVWAEAARATIAGHERDLQTLLPWASALE
                     PSADLVALRALRSSALTPADLPGRCQVAIAELTTLRETAVREDPAGEEAIARLDQAID
                     GLRRSSSAAEALVGRLQEIARATRELAAEMEFGFLFDPARKIFSIGYRVSDGTLDPSA
                     YDLLASEARLASFMAIAKGDVPVSHWFHLGRPMTPVGIGSALLSWSGSMFEYLMPALV
                     MRSPVGSLLEETYRLVVRRHLTYGAENGVPWGISESAYNVRDLDLTYQYSNFGVPGLG
                     LERGLGEDMVVAPYATILAAMVDPGAATRNLSALVAVGARGPYGFYEALDYTRSRLPE
                     GTEVAIVRAYMAHHQGMSLVALANVLHDGVMRARFHAEPIVQATDLLLQERAPRDVAV
                     ARPRVEEVGAAAHARDFVEPVFRQFTTPHGPPRTHLLSNGRYAVMVTTAGSGYSRCAG
                     LGITRWREDVTRDNWGSYVFVRDVHSGAVWSAGYQPTGAEPDSYRVTFSEDRAEFHRR
                     DGAITTTLEVFVSPEDDAEMRRVSITNLGLQTRELELTSYAEIVLAPPAADAAHPVFS
                     SLFVQTESLPDLGALLATRRPRSAAEPAVWAAHTVVVEGQAGGGAQYETDRGRFLGRG
                     RSIRTPMSVVDGRPLSNTAGSVLDPIFSLRRRIRLGAGESARLIFSTLVAPSRDAAVA
                     LADKYRDPATFERTVTLAWTQAQVQCHHLGIDPDEAHLFQNLASRVLFSDPALRPSAD
                     VLSRITSGPSALWAQQISGELPIVLVRIDEPEDRAIVRQLLRAHEYWRVKGLAVDLVI
                     VNEKAHSYTQELQASLEALLRTSQSARRDEREDARGNVFILRKDLLSPDDHAALQVAA
                     RVVLLSRHGTLTEQLARAEPPPDPATPRRRPPRPEALGNVPSPRQESEFFNGLGGFVD
                     DGREYVTVLGEGQWTPAPWVNIVANPAFGFQVSESGAGYTWALNSRENQLTPWSNDPV
                     ADPPGETIYIRDEETGELWTPTLLPIREDASVYVARHGQGYTCFEHTSHEIAARLVQF
                     VPREDPIKISRLTLENRSGRSRRLSVTAYVEWVLGALRSTTAPFVITEIDSRTGALFA
                     RNAWSPEFGDQVAFADLGGRQTAWTGDRTEVLGRNGTLDHPAGLERGARLSGRIGAGL
                     DPCAALQTVIDVPPGGREEIVFFLGEAASSADARALLDRYRGEDLDSTLKTVRTEWDD
                     TLGAVQVKTPDRSMDLMLNRWLLYQTLGCRVWARSAFYQAGGAYGFRDQLQDVMALAV
                     AKREVAREHLLRAAARQFIEGDVQHWWHPPSGRGVRTRISDDSLWLPYALTHYLEVTG
                     DTAILDEIVPFLEGPAIPPGQEDAYFQPALSAERATLFEHCARALDRSLPVGSHGLPL
                     IGTGDWNDGMNRVGHEGRGESVWLGWFLHTTLWEFARLADARGENGRADTWRKHVHAL
                     KASLEARAWDGDWYRRAYFDDGTPLGSVDNAECRIDSIAQSWGVISGAADPARAARAM
                     AAVEEYLVRRDQELILLFTPPFDHAPLDPGYIKGYPPGIRENGGQYTHAAIWAVMAFA
                     ALGNGDKANELFSILNPINRTSTRAGVHRYKVEPYVAVADVYAEYPHVGRGGWSWYTG
                     SAGWMYRAGLEWLLGFRLRGAVLHLDPCIPRAWRRFEITFRYHASRYEIEVLNPNGAT
                     RGISTIDVDGIPQSVAGGSVPLTSDGATHRVRVVLG"
     gene            8972..9286
                     /locus_tag="VNU03_23720"
     CDS             8972..9286
                     /locus_tag="VNU03_23720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77553.1"
                     /translation="MKLGDLTHRTGGTVWPGRWVESLGPGDASAVPEDGILEGLMRLG
                     GRLLLRINVNGQRRTASLEWEPPPAVGDVESVLLASMGAEIRQLGLLELPSRGERARP
                     IP"
     gene            complement(9313..9495)
                     /locus_tag="VNU03_23725"
     CDS             complement(9313..9495)
                     /locus_tag="VNU03_23725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77554.1"
                     /translation="MLPPLPKEGPRRVCDGCGKANFLPYSIRTGEPVSATERRYCSLS
                     CAQLHFPSFKGGSSGR"
     gene            9740..10009
                     /locus_tag="VNU03_23730"
     CDS             9740..10009
                     /locus_tag="VNU03_23730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77555.1"
                     /translation="MYRGDRRRKEDQRKAKQEAKRARKVERRESGASGPEIEAAGAGG
                     PASKEFVWFSPSKGRTQTTTTAAVPSIDGVDDWTLVSDPRLNPGA"
     gene            complement(10046..10585)
                     /locus_tag="VNU03_23735"
     CDS             complement(10046..10585)
                     /locus_tag="VNU03_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWP77556.1"
                     /translation="MLWKILIALVVIVVGLVTVIALQPARYRVSRSTTIAAPAPVVFG
                     QVNDFHRWTAWSPWEKIDPAMKRTYEGPPAGVGASYTWVGSGEVGEGRMTIVESRPSD
                     LIQVKLEFVKPFAGTSVAEFSFKPEGDRTLVTWSMTGDKNFIAKAIHLVMSMDRMIGD
                     QFDKGLAAMKTVAEAAPRP"
     gene            10730..11617
                     /locus_tag="VNU03_23740"
     CDS             10730..11617
                     /locus_tag="VNU03_23740"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWP77557.1"
                     /translation="MPPLLAWASLGTGFVIALAAVLRMAGSVPEGDHVTGAIRLPGPV
                     TAAILALFGLALVVFLGDLVRRALAKRDREDDGAPAEEPTPVPSWLRRVTLILSLVNV
                     AVLAYLWRRAVLEGGLFVGAGGLASGLGLPDMEVLTAPALFNWVFGSLAVMAGLGALG
                     LALWSALGDRLVPDGRDADSAMPAAPLRTAVEESLDDLRAEGDPRRAIVRCYARFERV
                     AADSGLERRPWLTPTEFMREVLARLSLPRAAVPTLTGLFELARFSHHPLGPSERDRAV
                     DALHEIRRATAPSEGDAAS"
     gene            11655..12059
                     /locus_tag="VNU03_23745"
     CDS             11655..12059
                     /locus_tag="VNU03_23745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77558.1"
                     /translation="MSLLAVAALPVYLTISPAWRPAAARLACTVVVAIGCARARAWAR
                     AAVATPGLSLFEAPPEPAPELTLDPRFLRLRDDLVASTRRRRYFDVVLWPRLSALAGP
                     DLPRPAEPRGPIRRGPSLAAIERLVARIEGCP"
     gene            12056..13012
                     /locus_tag="VNU03_23750"
     CDS             12056..13012
                     /locus_tag="VNU03_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012572645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWP77559.1"
                     /translation="MKLETVADRGQDVLAALGQVIVGKVDVLRQILAGILANGHILIE
                     DYPGLAKTLIARLVSQSLDLGFKRIQFTPDLLPSDITGSFLYDQREGRFEFRRGPVFT
                     NLLLADEINRATPKTQSALLEAMQESQVTVEGERFPLEAPFLVIATQNPIELEGTYPL
                     PEAQLDRFLMRLRVGYPDRDDEVEILARRRRRREDAVGIAPVISRAELGAMQGCLEEV
                     HVAAVTERYIVDLVQATRSDARVALGASPRGTLALLKLARATAALARRDFVLPDDVKA
                     MAVPALAHRLILKPELWAARISATQVVESLLEQVPAPGPEPR"
     gene            13009..14286
                     /locus_tag="VNU03_23755"
     CDS             13009..14286
                     /locus_tag="VNU03_23755"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWP77560.1"
                     /translation="MTGRVTPLALSILAVAGWAVSLGVLLARPELFLVALPGVLVLVA
                     LGRRASRPACSIAHRISSDRLFEGDALTVTVTVGARAPVALVELLGPLPPGAVLGSGR
                     PRALMTLRAGDAVEWSYDVRFPRRGVHDLGAITVRVRDRLGLRTWEYRHVTPTVVRVY
                     PRIVPLRRLPRPFHTQPSVGDYVSSALGEGIEPGDVRQFAPGDRIKQVNWRASLRLGQ
                     LYVTQRHRERNADVVLMLDTLAEVGAPPETTLDQGVRAAASLATAYLARKDRVGLITY
                     GGVLSWVRPGSGPVQYERLADGLVRADVVFTYVAKDLQRVPARVLSPQALVLAITPLL
                     DRRFAKAVLDLAARGFDLIVLVVSPVAVTRAAMPPSPADDLAGRLWALERRADLTELR
                     RHGLPVLEWCPPEPLEAALVSVPRRRVRLASAG"
     gene            14283..14792
                     /locus_tag="VNU03_23760"
     CDS             14283..14792
                     /locus_tag="VNU03_23760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77561.1"
                     /translation="MTRLLAAAAAGALFLLLRLTTPAPPVIGVGLLAVGLSAVAIAVA
                     WRWAATSAAVVFLIGYAAALGIERRPVDVGAALGFGLALVLLLGAVDLSARARGASLH
                     GRVVGATLGRFIGLGAGALAAALLAMAVATGVATALTSTAAPLLAAAGALAFVWSLAA
                     LVRRAAGPS"
     gene            complement(14797..15192)
                     /locus_tag="VNU03_23765"
     CDS             complement(14797..15192)
                     /locus_tag="VNU03_23765"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP77562.1"
                     /translation="MIRAVDVTAELAGLTTLHGRSASTPADRVKAAFAVLAPFRDGSV
                     FAGRFSGETAWERHAKGDELVQILDGAATLTIMTADGPQAVALSAGMLIVVPQGHWHR
                     FEAPDGVTVLSATPQPTEHTAAADPRDAG"
     gene            complement(15192..16073)
                     /locus_tag="VNU03_23770"
     CDS             complement(15192..16073)
                     /locus_tag="VNU03_23770"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77563.1"
                     /translation="MKIRIGVGAGGASASAEALGELVGSLDGLGFDSLWLSEVLTGPV
                     LDPMVGLAWAAASNPRLKIGTTALLPGRNVLRLAKQLASLDRLSGGRLLVTLVPGLTY
                     PPEREAIGVEPKQRGAVIDDALPLLRRLWAGETVSHDGPAGHFHDVRLSPLPVQQPLE
                     VWLGGNVPAALERCGRLSDGWLPSLCTPEEAAAGRTVIEEAAARAGRSISPEHFGMSV
                     GYASAPIDPDTARTMAARRPRSLELTPVGYPALRKTLEQFIAVGFSKFVVRPVAAPRS
                     WRAELEALAAAVGDLQT"
     gene            16127..>16617
                     /locus_tag="VNU03_23775"
     CDS             16127..>16617
                     /locus_tag="VNU03_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease"
                     /protein_id="HWP77564.1"
                     /translation="MIVHLVDGTYELFRYFLSPAAAFDRSAPPELRAVRGVVGSILGM
                     LEEGATHVGVATDHVIESFRNALWPGYKTGEGIDPLLYAQFEPLEEALAALGVVVWAM
                     VEFEADDALATAATMAAADARVEQVIVCTPDKDLAQCVRGDRVVQLDRRRREIRDEAG
                     VVK"
BASE COUNT         2264 a         5763 c         6271 g         2319 t
ORIGIN      
        1 gcgcctcgcc attcgcaacc tgatgcagga cctgcgggcg accggcacgc tcatcgacgg
       61 gcccgccccg ttcggcccgc gggagcgtcg cctcttcgcc gatcacctcg accacctgct
      121 gacccgacgc cggcccgcct agggcgccgc ggtccgccgg ctcacccgag gacgacccgc
      181 acccggtggg tcgcgccgtc gctcgtgagc gggacgctgc cgcccgcgac cgactgggga
      241 atcccgtcga cgtcgatggt cgagatcccg cgggtcgccc cgttcgggtt cagaacctcg
      301 atctcgtagc gcgacgcgtg gtaccggaac gtgatctcga agcgacgcca cgcgcgcggg
      361 atgcaggggt cgagatggag cacggccccc cggagccgga agccgagcag ccactcgagc
      421 ccggcccggt acatccagcc cgcggagccg gtataccagc tccacccccc gcgcccgacg
      481 tgggggtact cggcgtagac gtcggcgacc gcgacgtagg gctcgacctt gtagcgatgg
      541 acgccggcgc gggtgctcgt gcggttgatc gggttcagga tcgagaagag ctcgttggcc
      601 ttgtccccgt tcccgagcgc ggcgaaggcc atgaccgccc agatggcggc gtgggtgtac
      661 tggccgccgt tctcccggat gccgggcgga tatcccttga tgtagccggg gtcgagcggc
      721 gcgtgatcga acgggggagt gaagagcagg atcagctcct gatctcgccg gaccagatat
      781 tcttccaccg cggccatcgc gcgcgccgcg cgcgcggggt cggccgcgcc ggagatcaca
      841 ccccacgact gcgcgatgga gtcgatgcga cactccgcgt tgtccaccga cccgagcggg
      901 gtaccgtcgt cgaagtaggc tcggcgatac cagtcgccgt cccacgcgcg ggcctcgagc
      961 gaggccttga gggcgtgcac gtgcttgcgc caggtgtcgg ctcggccgtt ctccccgcgg
     1021 gcgtcggcga gccgcgcgaa ctcccagagc gtcgtgtgca ggaaccagcc gagccaaacg
     1081 ctctcccccc gtccttcgtg gcccacccgg ttcatgccgt cgttccagtc gccggtaccg
     1141 atcaggggga ggccgtggga tccgaccggg aggctgcggt ccagggcgcg ggcacagtgc
     1201 tcgaagaggg tggcgcgctc ggcggagagg gcgggctgga agtaggcgtc ctcctgtccg
     1261 ggcgggatgg cgggtccctc gaggaagggg acgatctcgt ccaggatcgc ggtgtccccg
     1321 gtgacctcga ggtagtgcgt gagcgcgtag ggcagccaga gcgagtcgtc cgagatccgg
     1381 gtccgcacgc cccgaccgga cgggggatgc caccagtgct ggacgtcgcc ctcgatgaac
     1441 tgccgggccg cggcccggag caggtgctcg cgcgcgacct cgcgcttcgc gacggccagc
     1501 gccatcacgt cctggagctg atcgcgaaag ccgtaggcgc cgcccgcctg gtagaaggcg
     1561 gatcgcgccc acacccggca gccgagggtc tggtacagga gccaccggtt cagcatgaga
     1621 tccatggagc gatccggggt cttcacctgg acggcgccga gggtgtcgtc ccactcggtt
     1681 cgaacggtct tcaacgtgga gtcgaggtcc tccccgcgat agcggtcgag gagggcccgc
     1741 gcgtcggccg acgaggcggc ctcgccgagg aagaacacga tctcctcgcg gccgccgggc
     1801 ggcacgtcga tcacggtctg gagcgcggca cacggatcga ggcccgcccc gatccgtccg
     1861 gagagccggg ctccccgctc cagacccgcg ggatggtcca gcgtcccgtt gcgaccgagg
     1921 acctcggtcc gatcgccggt ccacgcggtc tggcgaccgc cgagatcggc gaacgcgacc
     1981 tgatcgccga actccgggct ccaggcgttg cgggcgaaca gcgccccggt ccgggagtcg
     2041 atctcggtga tcacgaacgg cgcggtcgtg ctgcggagag cgccgagcac ccactcgacg
     2101 tacgcggtca ccgacaggcg acgcgagcgg ccggagcggt tctccagggt cagccgcgag
     2161 atcttgatcg ggtcttcgcg cggcacgaac tggacgaggc gtgccgcgat ctcgtgcgag
     2221 gtgtgctcga agcaggtgta gccctgaccg tgccgggcca cgtagaccga ggcgtcctcg
     2281 cggatgggga ggagggtcgg agtccacagc tcgcccgtct cctcgtcccg gatgtagatc
     2341 gtctcgccgg gcggatcggc gaccgggtcg ttggaccacg gggtcagctg gttctcacgg
     2401 ctgttgagcg cccaggtgta cccggcgccc gactcggaga cctggaagcc gaacgccggg
     2461 ttggcgacga tgttgaccca gggcgccggg gtccactgtc cttccccgag gacggtgacg
     2521 tactcgcgcc cgtcgtcgac gaagcctccg agcccgttga agaactcgga ttcctgtcgc
     2581 ggagagggga cattcccgag cgcttccggc cggggtgggc gccgccgcgg agtggccggg
     2641 tctggaggcg gctcggctcg agcgagctgc tcggtgagcg tcccgtgtcg actcaggagg
     2701 acgacccggg ccgccacctg cagcgcggcg tggtcgtccg gcgagagcag atccttccgg
     2761 aggatgaaga cgttcccgcg ggcgtcctcc cgctcgtccc gtcgcgcgga ctggctcgtg
     2821 cggagcagcg cctccagcga ggcctggagc tcctgggtat aggagtgggc cttctcgttc
     2881 acgatgacga ggtcgaccgc gaggcccttc acgcgccagt actcgtgcgc ccggaggagc
     2941 tggcggacga tcgcccggtc ctcgggctcg tcgatccgga ccaggacgat cgggagctcg
     3001 ccggagatct gctgggccca cagggcggat ggccccgacg tgatccggct cagcacgtcc
     3061 gccgacggtc gcagcgccgg gtcggagaag aggacccggc tggccagatt ctggaagagg
     3121 tgggcctcgt ccggatcgat gccgaggtgg tggcactgga cctgcgcctg ggtccaggcc
     3181 agagtgacgg tgcgctcgaa cgtcgccggg tcgcggtact tgtcggccag cgccaccgcg
     3241 gcgtcgcgcg agggggcgac gagagtggag aagatgaggc gggcgctctc tcccgcgccg
     3301 agtcggatcc gacgtctcag gctgaagatc ggatcgagga ccgagccggc ggtgttcgac
     3361 aggggtcgcc cgtcgacgac cgacatcggc gtacggatgc tccgcccccg accgaggaag
     3421 cgtccccggt cggtctcgta ctgcgcgcct ccgccggcct gcccctcgac gaccacggtg
     3481 tgggcggccc agacggcggg ctccgccgcg gagcgggggc ggcgggtggc cagcagggca
     3541 cccagatcgg ggagggattc cgtctggacg aagagactgg agaagaccgg atgggcggcg
     3601 tccgcggcgg ggggagccag cacgatctcg gcgtaggacg tcagctccag ctcgcgcgtc
     3661 tgcagcccga gattcgtgat cgagacccgg cgcatctcgg cgtcgtcctc gggcgagacg
     3721 aagacctcga gcgtggtcgt gatcgcgccg tcgcgacgat ggaactccgc ccggtcctcc
     3781 gagaaggtca cccggtagct gtcgggctcg gctccggtgg gctggtagcc ggccgaccag
     3841 accgcgccgc tgtggacgtc gcgcacgaag acgtagctcc cccagttgtc ccgcgtgacg
     3901 tcctcgcgcc agcgggtgat cccgaggccg gcgcatcggc tgtagcccga gcccgccgtc
     3961 gtcaccatca ccgcgtatcg gccgttcgac agaaggtgcg tgcggggagg gccgtggggc
     4021 gtcgtgaact gacggaagac cggctcgacg aagtcgcgcg cgtgggccgc cgcgcccacc
     4081 tcctcgaccc gcgggcgcgc caccgcgacg tctcgtggag cccgctcctg caggagcagg
     4141 tcggtcgcct ggacgatcgg ctcggcgtgg aaccgcgcgc gcatcacccc gtcgtggagc
     4201 acgttggcga gcgcgacgag gctcatgccc tggtggtgcg ccatgtaggc ccgcacgatg
     4261 gccacctcgg tgccttcggg gagccgcgat ctcgtgtagt cgagcgcctc gtagaagccg
     4321 tacggccccc gggcgcccac cgccacgagc gccgagaggt tgcgggtggc ggcccccggg
     4381 tcgaccatcg cggcgagaat cgtggcgtag ggcgcgacga ccatgtcttc gccgaggccc
     4441 cgctcgagcc cgaggcccgg caccccgaag ttcgagtact ggtaggtgag atccagatcg
     4501 cgcacgttgt acgccgactc cgagatgccc caggggacgc cgttctccgc gccgtacgtc
     4561 aggtgacggc ggaccacgag gcggtaggtc tcctccagga ggctcccgac cggggagcgc
     4621 atgacgagcg ccggcatcag gtactcgaac atcgagcccg accacgaaag gagagccgac
     4681 ccgatgccga ccggagtcat cggccgaccg aggtggaacc agtgcgagac cggcacgtcg
     4741 cccttggcga tggccatgaa gctggcgagc cgggcctcgg acgcgagcag gtcgtacgcg
     4801 ctcgggtcga gggtgccgtc gctgacgcgg tagccgatcg agaagatctt gcgggcgggg
     4861 tcgaagagga atccgaactc catctcggcg gcgagctccc gcgtggcgcg agcgatctcc
     4921 tggagtcgcc cgacgagcgc ctcggcggcc gaggaggacc gccgaaggcc gtcgatggcc
     4981 tgatcgagtc gcgcgatcgc ctcctccccc gcggggtcct cgcggacggc cgtctcgcgc
     5041 agggtcgtga gctcggcgat cgcgacctgg cagcggcccg gaaggtcggc aggagtgagc
     5101 gccgaggatc ggagggcccg gagcgccacc aggtcggccg agggctcgag ggcggacgcc
     5161 cagggcagga gcgtctggag atcgcgctcg tggccggcga tggtggcgcg cgcggcctcg
     5221 gcccacacct gcacgtcgga gtcgaccgga tccccgcgct cggcggtcag cgcgcgcgac
     5281 atgtccacga gggtgtccgc gtggagggcc aggtgacgga cgcggccgga ccattccgcc
     5341 ggatcggccg gcggctcatg gagcgccgcg atcaggaccg cgcaggtctc ggcgagatga
     5401 ccgcggggga cggtctgggt gcgccggtca tcggcgagca cgctggcggc ttcctggacg
     5461 aggagcaccg cgtcccggag cccgccgaac cggccgggat cccgcagggg ggcgtcgatc
     5521 gcggcgcggc aggcgttgcc gagggtcagc aggtgcgcgg cgaggttgcc gctgtcgacg
     5581 gtggacacgt agcggggctc cagcggccgg aggccgccgc cggtgtcgta ccagttgtag
     5641 aagtggcccc ggaagcggtc gaggcgatgc agcgtggcga gggtggcctc cagacggtcg
     5701 acagtgtcga gaatcccgag ccagccgaaa tcgtgcgcgg agacgaccgc gagcaggtag
     5761 agcccgatgt tggtgggcga cgtgcgatgg gcgacggtcg gcttcggatc ctcctgaaaa
     5821 ttgtcgggcg ggaggaaggc gtcgtcgggg ccgacgaagg tctcgaagaa gcgccaggtc
     5881 cggcgggccg tcgagcggag gatctggcgc tccgagaggg agaggatctg ggtcgtcgac
     5941 gggcgggggg cgcggctcac ccagcgggcg gccagcgggg agagcgccca ggccaggatg
     6001 aacggtgcgg cgatcgcggc gtgctccggg cgcaggagcg cgacgagggc gccggccgcg
     6061 acggcgagga tcaccggcac ttgcatgcgc cgatagacgg aagtgatgtc gcgggccagg
     6121 tcggacttgg cctgggccgc ggtcgtccac tccagcagac gtcgacgggt caggtacagg
     6181 cggacgagcg tccgcgcgat cgcgtcggcc atcagccacg cctggtgggc gagaaaggtg
     6241 atgccgaggg cgatctgggt ggacgcgaga gcgaagctcc gtcccacgct gcggatgtgg
     6301 gtccgcttcg acactcccga tcgtccgggg atgacctcgg tgagcgcggg caggagagcg
     6361 gggagtccga gggtggccag aatgaacgcg gtccacacga gggccgagct gccgggaagc
     6421 gcccaggcgg cgacgagggt cagccaggtg gccgggggcg acaaggtccg gcgcaggttg
     6481 tcgacgatct tccacgcgcc gatgagcggg aggccgcctc cgcgctcccc gccgccgacg
     6541 tctcccttga tgatccacgg gaggagctgc cagtcgcccc gcgcccagcg gtactgacgg
     6601 ccggccgcga cctcgtagtc ggtggggaac tcctcgaaga gctcgatatc ggtcacgagt
     6661 ccggcccggg cgaagatgcc ttcgaacaga tcgtggctca ggagggagtt ctcggggacc
     6721 cggccctcga gggccgcggt gaaggcggcc acgtcgtaga tgccctttcc ggtgtacgag
     6781 ccctcgccga ggaggtcctg atagacgtcg gaggtcgcgg aggcgtaggg gtcgatgccg
     6841 gcgggcccgg caaagatccg ctggaagagg gacgtgccgc gctcggtggg gatggtggcg
     6901 gtgacccgcg gctggaggac ggcgtaaccg tcgacgacgc ggcccgttcg ctcgtcgatg
     6961 gcgggccggt tcagcggatg ggccatggtg ccgaccagcc gggtcgcggc gccacggggc
     7021 aaacgggtgt cggcgtcgag ggtgatcacg tagcggaccc ccgagggcac gtggggggct
     7081 cgcccgtggg tcgcggtgaa cgtcgtatca ctcgcgcccg cgagcaggtg gttcagctcg
     7141 tcgagcttcc cccgcttgcg ctcccatccc atccacttgg cctcgcccgc gttccagagt
     7201 cgcttgcgat ggagcaagag aaagcgcgaa cccgccgggg gcgcgggacc gttgcgcgcg
     7261 ttgagccggg cgatgccatc gcgggcggcg gccaggagcc ggtcgtcctc cggcatcgcc
     7321 tcggtgggcg cgtcgaccca gtcggagagg agggcgaagt ggaggtctcc ggccgggttg
     7381 gcgaggaagt ggatctcgag ccgtccgacc tgctcctcga tgtcggcttc gctcgtcagc
     7441 agcgtcggca ccaccacgag ggtccgcagg cccgggggaa cgccgtcgcg cagctccaga
     7501 cgcggcagcg acagcgggcc gagcaccgcc atgaccgcgc gattgacgag cgcgaccgcg
     7561 aggtcggagg cggggatgac cgcgaacagg gtcagcagga tcagcccgcc gagagccatg
     7621 ccggaggcgt gcccgatcag gaggggcagc gccaggagcg cgccggtcac cgccacgatg
     7681 gtggccaggt agcggggggt cgcgctggtc agaaaggcgc gcagcagcca gcgcctcacc
     7741 gggacgtgat agccgatctc cttctcgagg acccggcggc cgtccgagat caggtagtag
     7801 cccggatcct cggcgcggcc cccgggcgac gcgccggacc gttgcgcggc ggagacggcc
     7861 cgcttggccg cggccatcgc caggcgggtg acctcgagct cggagcgcgc ggaggcgcgg
     7921 gcgagatcct cgatcgcgtg acggtactgg tcgcgggtcg cgaagtccat ggctccgaag
     7981 gaagatccgc cgcgcagcgt gtcgtcgacg aggctcacgc tctcgaagaa gtcggcccag
     8041 tcgagggtcg acatcatccg catgctggtg atcacgttgc ggaccgtcac gttcatcgcg
     8101 gcctggttct ggtgctcggt gtgcacgagc tcgtcgggcg actgtccgcg cgcggcgagc
     8161 tgctcgtcga gccatcgcag ggcgggggtc gtctgaggat cctggtcgcg caggcgctgc
     8221 acgagctcga ccgtgaaggc gggggacagg gcggcggcct cgaggccctg gaaggcgagg
     8281 ggtcgaccgg gccggccgcc caggccgagg aggtcgttcg cgagcgcgtc ggcggcctgg
     8341 cgcgcggcgc gtccggccac gatgcgctcg gcgagccgcc ggaggttctc cacgagcacg
     8401 atgcggaggg cgatcgggac cgcccagagc tccccgatgg tcaggggctg caccctctgg
     8461 aacgcgcgga cgaagcgctg gagggtctcg ggctcgagtc ggctgtcggt gtgcgcgacg
     8521 aaggcccacg ccagtccgag cacgcgggga tacccctgca gcgggctctc cgcgagcttg
     8581 ggcagctggc ggtagaaccc cggaggcaga tcctcgcgaa cctggcgcac caccgcgtcc
     8641 accacgtgga agttgtcgac gaaccactcc gcggccggag tcgtcgcccc ctcgtcgcgg
     8701 atcacggaag cgatctcgcg atagcacagg agcagaaccc gcccgctgtc ccggacccgg
     8761 ggcgagaggc ggcggccggc tcgctcgcgg ggcaggaccc gctggcgggc gatggactcc
     8821 gcgtgctcct cgagccgctc cgggctgaag agctcggccc ggatgggctc ctgggtcccc
     8881 ggcgtgaatg gggacgaggg agcggcgaaa agggggtcgc gcgctggcac tgagatctct
     8941 acagtgatat aagttgaata agaacaataa aatgaagctg ggcgatctca cgcatcgaac
     9001 gggagggacg gtctggccgg gccggtgggt ggaatcactc gggccgggcg acgcatccgc
     9061 ggtgcccgag gacgggatcc tggaggggct catgcggctg ggcgggcgcc tgctcctgcg
     9121 gatcaacgtc aacggccagc ggcgaaccgc cagcctggag tgggagccgc ctcccgcggt
     9181 gggagacgtc gagagcgtgc tgctggcgag catgggcgcc gagatccggc agctcgggct
     9241 cctggagctg ccgagccggg gtgagcgcgc tcgccccatc ccctgaggcc gcgcgggcga
     9301 cgcgcggccc tctcatctcc cggagctccc acccttgaag ctggggaagt ggagctgggc
     9361 gcagctcagc gagcagtagc ggcgctccgt cgcgctgacc ggctctccgg tccggatgga
     9421 gtagggcagg aagttggcct tgccgcatcc gtcacacact cgacgtggac cttccttcgg
     9481 aagtggcggt agcatgcgag atctcctcct tgtgcgagac gcctccaatc ctagcagcgc
     9541 acgcgcggca ggcctattgg accaatgtcc aatagcccgg cgggcggatc gggcaggccg
     9601 acacgcggag cggctcccgc ctgtgcaata ctcgggggcg gcgcgccgcc gcgcggcgcg
     9661 tttccgagga agtggttcgg cccgtcatgt aaagtaggga gagcgagaaa ggagcggaat
     9721 ggctcaaaaa agacccggga tgtaccgcgg cgatcgccgc cgcaaggaag accagcgcaa
     9781 ggccaagcag gaagccaagc gggcgcgcaa ggtggagcgc cgcgagagcg gggcgagcgg
     9841 gcccgagatc gaggcggccg gggcgggtgg gccggcctcg aaggaattcg tctggttctc
     9901 gcccagcaag ggacgcacgc agaccacgac caccgcggcg gtgcccagca tcgacggggt
     9961 ggacgactgg acgctggtga gcgatccgcg gttgaacccg ggcgcctgag cccggggcct
    10021 ccttcacgtc cccagcgtca gattcctagg gccggggcgc cgcttccgcc accgtcttca
    10081 tcgcggcgag gcccttgtcg aactgatcac cgatcatccg gtccatgctc atgacgaggt
    10141 ggatcgcctt cgcgatgaaa ttcttgtcgc cggtcatgct ccaggtcacc agggtccggt
    10201 cgccctcggg tttgaaggaa aattccgcga cgctggtccc cgcgaagggc ttcacgaact
    10261 cgagcttgac ctggatcagg tcgctcgggc ggctctccac gatcgtcatg cgtccctcgc
    10321 ccacctcgcc gctacccacc cacgtgtagc tcgccccgac gcccgcgggc gggccctcgt
    10381 aggtccgctt catcgcgggg tcgatcttct cccatggcga ccacgcggtc caccggtgga
    10441 agtcgttcac ctgcccgaag accaccgggg cgggcgcggc gatggtggtc gaccgggaga
    10501 cgcgataccg ggcgggctgc agcgcgatca ccgtcacgag tccgacgacg atcacgacga
    10561 gtgcgatgag gatcttccag agcatgcgcg tgtgctcctt tcgcactatg gtcgaacggt
    10621 ccgactcggg atcgacacgc gatcccgaaa accagttgcc agagaatgcc tcgatattac
    10681 tggcaaaaag accttgcgca gcgtcgcgcg aaccggcatg gtggcgcccg tgccgcctct
    10741 actcgcctgg gcctccctcg gcaccggctt cgtgatcgcc ctggccgccg tgctgcggat
    10801 ggccggctcg gtccccgagg gcgatcacgt gaccggcgcc atccggctgc ccgggccggt
    10861 gaccgcggcg atcctggctc tcttcggcct ggccctcgtg gtcttcctgg gtgatctcgt
    10921 gcgtcgcgca ctggcgaagc gcgaccggga ggacgatggg gcgccggcgg aggagccgac
    10981 cccggtcccg tcgtggctgc gccgggtcac gctgatcctg tccctggtca acgtcgcggt
    11041 cctcgcgtac ctgtggcgcc gggcggtgct cgagggcggg ctcttcgtcg gggcgggcgg
    11101 actggcgagc ggactggggc tgccggacat ggaggtcctg accgcgcccg cgctcttcaa
    11161 ctgggtcttc gggagcctcg cggtgatggc cggcctcggc gcgctcggac tcgccctctg
    11221 gagcgcgctc ggcgatcgcc tggtcccgga cggccgcgac gccgattccg cgatgccggc
    11281 ggcgccgctg cggaccgcgg tggaggagag cctggacgat ctgcgcgccg agggcgatcc
    11341 gcgtcgcgcc atcgtccgct gctacgcgcg gttcgagcgc gtggcggccg actccgggct
    11401 cgagcggagg ccgtggctca ccccgaccga gttcatgcgc gaggtcctcg cccgcctgtc
    11461 ccttccgcgc gcggcggtgc cgaccctcac cggcctgttc gagctcgcgc gcttcagtca
    11521 tcacccgctc ggcccgtccg agcgggatcg cgcggtcgac gccctgcacg agatccgccg
    11581 cgcgaccgcg ccgagcgagg gtgatgccgc cagctagaca cgcgggaccc cggctcctgg
    11641 cgcgtatcgt cgggttgagc ctgctcgccg tcgccgcgct gcccgtctac ctcacgatct
    11701 ctcccgcgtg gcggccggcg gccgcgcgtc tcgcgtgcac ggtggtcgtc gcgatcggct
    11761 gtgcccgcgc gcgagcctgg gcgcgggccg ccgtcgcgac gcccgggctc tctctcttcg
    11821 aggccccgcc ggagccggcg cccgagctga ccctcgatcc gcgcttcctc cgcctgcgcg
    11881 acgacctggt cgcgagcacg cggcgccggc gctacttcga cgtggtcctc tggccgcggc
    11941 tctccgcgct cgcggggccg gatctcccgc ggccggcgga gccgcgcggc ccgatccgcc
    12001 gcgggccctc gctcgcggcc atcgagcgtc tcgtcgcccg gatcgagggg tgcccgtgaa
    12061 gctcgagacg gtggccgacc gcgggcagga cgtgctcgcc gcgctcggcc aggtgatcgt
    12121 gggcaaggtc gacgtgctcc ggcagatcct ggccgggatc ctcgccaacg ggcacatcct
    12181 gatcgaggac taccccgggc tcgccaagac gctcatcgcc cggctcgtct cccagagcct
    12241 cgatctcggg ttcaagcgga tccagttcac gcccgacctc ctgcccagcg acatcaccgg
    12301 gagcttcctc tacgaccagc gggaggggcg cttcgagttc cggcggggtc ccgtcttcac
    12361 caacctgctg ctggccgacg agatcaaccg ggccacgccc aagacccaga gcgcgctgct
    12421 cgaggcgatg caggagtcgc aggtcaccgt cgagggcgaa cgcttcccgc tcgaggcccc
    12481 gttcctggtc atcgccaccc agaacccgat cgagctcgag gggacctatc cgctgccgga
    12541 ggcccagctc gaccgcttcc tgatgcgcct ccgcgtgggc tatccggacc gcgacgacga
    12601 ggtcgagatc ctggcccggc ggcgccggcg gcgggaggac gcggtcggca tcgccccggt
    12661 gatctcgcgc gcggagctcg gcgccatgca gggctgtctc gaggaggtgc acgtggcggc
    12721 ggtcaccgag cgctacatcg tggacctcgt gcaggccacc cgctccgatg cgcgcgtggc
    12781 cctcggcgcc tccccgcgcg gcaccctcgc gctgctcaag ctggcccgcg ccaccgcggc
    12841 gctcgctcgg cgcgacttcg tgctgccgga cgacgtgaag gccatggcgg tccccgccct
    12901 cgcccaccgg ctgatcctca agcccgagct gtgggcggcc cgcatctccg cgacgcaggt
    12961 cgtggagagt ctgctggagc aggtgccggc gccagggccg gagccccggt gacgggccgg
    13021 gtcaccccgc tcgcgctgtc gatcctggcc gtcgccgggt gggcggtctc gctcggggtc
    13081 ctgctcgcgc gcccggagct gttcctggtc gcgctgccgg gcgtgctggt gctcgtcgcg
    13141 ctcggccgcc gggcctcgcg gccggcctgc tccatcgcgc accggatctc gagcgatcgc
    13201 ctcttcgagg gcgatgcgct gaccgtgacc gtgacggtcg gggcgcgcgc gcccgtcgcg
    13261 ctcgtcgagc tgctcggccc gctgccgccc ggcgccgtgc tcggctcggg ccgcccccgc
    13321 gcgctgatga ccctgcgcgc cggcgacgcg gtcgagtgga gctacgacgt gcgcttcccg
    13381 cggcgtgggg tgcacgatct cggggccatc acggtccggg tccgcgaccg gctcgggctg
    13441 cgcacctggg agtatcgcca cgtcaccccg acggtggtgc gggtctatcc gcggatcgtc
    13501 ccgctccggc gcctgccgcg tccgttccac acccagccgt cggtcggcga ctacgtctcc
    13561 tccgcgctcg gggaaggcat cgagccgggc gacgtgcgcc agttcgcgcc cggcgaccgg
    13621 atcaagcagg tcaactggcg ggcgtcgctg cgcctgggcc agctctacgt cacccagcgg
    13681 catcgcgagc gcaacgcgga cgtcgtgctc atgctcgaca cgctcgcgga ggtgggcgcg
    13741 ccgcccgaga ccacgctcga ccagggcgtg cgcgcggcgg cctcgctggc caccgcgtat
    13801 ctcgcccgga aggaccgcgt cgggctcatc acctacgggg gagtgctgag ctgggtgagg
    13861 cccggctcgg gaccggtcca gtacgagcgc ctggccgacg gcctcgtgcg ggccgacgtg
    13921 gtgttcacct acgtggccaa ggatctgcag cgcgtcccgg cgcgggtgct gtcaccccag
    13981 gcgctggtgc tcgcgatcac gccgctgctc gaccggcgct tcgcgaaggc ggtgctcgac
    14041 ctcgcggcgc ggggcttcga cctgatcgtg ctggtcgtct ccccggtcgc ggtcacgcgc
    14101 gccgcgatgc ctccgtcgcc cgccgacgac ctggccggac ggctctgggc cctcgagcgc
    14161 cgggcggatc tcaccgagct gcgccgccac gggctgccgg tgctcgagtg gtgcccgccg
    14221 gagccgctcg aggcggctct cgtgtcggtg ccgcggcggc gcgtccgcct cgcgagcgcg
    14281 ggatgacgcg gctcctcgcc gccgcggcgg ccggcgcgct cttcctgctc ctccggctga
    14341 ccacgccggc gccgccggtg atcggggtgg gtctgctcgc ggtcggcctg tcggcggtgg
    14401 cgatcgcggt ggcctggcgg tgggccgcga cgtccgccgc ggtcgtgttc ctgatcggct
    14461 acgcggcggc gctcggcatc gagcggcggc ccgtcgacgt gggggccgcg ctcggcttcg
    14521 ggctcgcgct ggtcctcctc ctgggggcgg tcgatctttc cgcgcgcgcc cgcggggcga
    14581 gcctccacgg tcgggtcgtc ggtgcgacgc tcgggcgctt catcgggctc ggcgccgggg
    14641 cgctcgcggc cgcgctgctg gccatggcgg tggcgaccgg ggtggcgacc gcgctgacct
    14701 cgacggccgc cccgctgctc gcggcggcgg gggcgctcgc cttcgtctgg tcgctcgcgg
    14761 ccctcgtccg gcgcgcggcc ggcccgagct agaccgctag cccgcgtctc gcggatccgc
    14821 ggcggcggtg tgctcggtgg gctggggggt ggcgctcagg accgtcacgc cgtcgggcgc
    14881 ctcgaaccgg tgccagtgtc cctgcggcac cacgatcagc atgccggcgg agagcgcgac
    14941 ggcctgcggc ccgtcggcgg tcatgatcgt gagggtcgcg gcgccgtcga ggatctgcac
    15001 cagctcgtcg cccttggcgt gccgctccca ggcggtctcg ccggagaagc ggcccgcgaa
    15061 gacgctcccg tcgcgaaagg gcgccagcac cgcgaaggcg gccttcaccc ggtcggccgg
    15121 cgtgctcgcg ctgcgtccgt ggagcgtggt gagcccggcc agctcggcgg tcacgtcgac
    15181 cgcgcggatc atcaggtctg cagatcgccg accgcggcgg cgagcgcctc gagctcggcg
    15241 cgccacgagc ggggcgcggc caccggccgc accacgaact tcgagaagcc gaccgcgatg
    15301 aactgctcca gcgtctttcg cagcgcgggg tagcccaccg gggtcagctc gagcgagcgg
    15361 ggccggcggg ccgccatcgt gcgcgcggtg tccggatcga tgggcgcgct cgcgtagccc
    15421 acgctcatgc cgaagtgctc cgggctgatc gagcgcccgg cgcgtgcggc ggcctcctcg
    15481 atgacggtgc gcccggccgc ggcctcctcc ggcgtgcaga gcgacggcag ccagccgtcc
    15541 gagagccgcc cgcatcgctc gagcgcggcg ggcacgttgc cgccgagcca cacctcgagc
    15601 ggctgctgca cgggcagggg cgacaggcgg acgtcgtgga aatgcccggc ggggccgtca
    15661 tggctcaccg tctcgccggc ccagaggcgg cgcagcaggg gcagcgcgtc gtcgatgacc
    15721 gccccgcgct gcttcggctc gaccccgatg gcctcgcgct ccgggggata ggtgaggccg
    15781 ggcaccaggg tgacgagcag gcgcccgccg gacagccggt cgaggctcgc gagctgcttg
    15841 gcgagccgca gcacgttgcg acccgggagc agcgcggtgg tcccgatctt gagccgcgga
    15901 ttgctcgcgg cggcccaggc gaggccgacc atcggatcga gcaccgggcc ggtcagcacc
    15961 tcggagagcc agagggaatc gaagccgagc ccgtcgagac ttcccaccag ctccccgagc
    16021 gcctccgcgc tcgcggacgc gcccccggcc cccaccccga tgcggatctt catggcgagg
    16081 ccgattctac cgcgtccggt ggccgccgtg gcgtatcgtt ctggccgtga tcgtccacct
    16141 ggtcgacggc acctacgagc tcttccgcta cttcctctcg cccgccgccg cgttcgatcg
    16201 gagcgcgccg ccggagctgc gcgcggtgcg cggcgtcgtc ggctcgatcc tgggcatgct
    16261 ggaagagggc gcgacccacg tgggcgtggc caccgatcac gtcatcgagt cgttccgcaa
    16321 cgcgctctgg cccggctaca agaccggcga gggcatcgac ccgctgctct acgcccagtt
    16381 cgagccgctg gaggaggcgc tggccgcgct cggagtggtc gtctgggcca tggtcgagtt
    16441 cgaggccgac gacgccctcg ccacggcggc gaccatggcg gcggccgacg cgcgggtgga
    16501 gcaggtgatc gtgtgcacgc cggacaagga cctggcccag tgcgtccgcg gcgaccgggt
    16561 cgtgcagctc gatcgccgcc gacgggagat ccgcgacgag gcgggggtgg tgaagaa
//
LOCUS       DATMCV010001036         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_855477, whole genome shotgun sequence.
ACCESSION   DATMCV010001036 DATMCV010000000
VERSION     DATMCV010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855477"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VNU03_23780"
     CDS             <1..923
                     /locus_tag="VNU03_23780"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77565.1"
                     /translation="VLALRADVADRAQLTAALAEGRKRFGAIHGVVHAAGIAGGGIIQ
                     LKTPEIADPVLQAKVQGTLALSELLADPELDFLLLCSSITAVMGGPGQVDYCGANAFL
                     DAFARSRAGVPGPRVHSVNWDAWQEVGMAANTRVPAALAARRARELADAILPAEGVDV
                     FDRVLESPLVEVIVSTRDLPRLIALARQMAEAPEAAVPETAPAAPASAGGRAGLPIPF
                     VAPTTELERTIAEVWQELLGIQPIGTQDDFFEAGGHSLLATQVMSRLHQRLGVDVPLR
                     VLFEARTVAAFAERVDEVSRSAAEREEIEL"
     gene            920..>2109
                     /locus_tag="VNU03_23785"
     CDS             920..>2109
                     /locus_tag="VNU03_23785"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HWP77566.1"
                     /translation="MTPAAELLSRLRALDVKISVEGGNLRVSAPKGAVTSALQQELAS
                     LKPEIIEVLKQRGDDATSGPTRMARAGDVPLSFVQQRLWFLDQLEPGTSTYTIAVRRR
                     LRGPLDESALTRALTELVRRHESLRTTFVTRNGEPRQQIMPAEPVVPETIDLESVPAA
                     DREAVVEEHVHKEVQRPFSLARGPLFRPVLLRLAPDEHELIASIHHIVADGWSLSPIA
                     RELETLYQAYTTGAEPALPELPLQYADFAIWQRQWLTGEVLEAHRAYWREHLAALPAP
                     LELPTDRPRARQSSPAAASHDFTLPPALANALRQLGRREGVTSFMTMLAGFKAVLARC
                     TGATDIVLGTPVANRHHVEIERIVGFFANTLVLRTSLDGDPSFRELLRRVREMSLGAY
                     AHQD"
BASE COUNT          290 a          772 c          761 g          286 t
ORIGIN      
        1 aggtgctggc gctccgggcg gacgtggccg atcgcgcgca gctgacggcg gcgctcgccg
       61 aggggcggaa gcggttcggc gccatccacg gagtcgtgca tgcggcgggc atcgcgggag
      121 gcgggatcat ccagctcaag accccggaga tcgccgaccc ggtgctccag gcgaaggttc
      181 agggcacgct cgcactctcg gagctgctcg ccgatccgga gctcgacttc ctgctcctct
      241 gctcctcgat caccgcggtg atgggggggc cgggacaggt cgactactgt ggcgcgaacg
      301 cgtttctcga cgccttcgcg cgcagccgtg ccggggtgcc cggtccacgc gtgcactcgg
      361 tgaactggga cgcctggcag gaagtcggca tggccgcgaa cacgcgggtg ccggcggcgc
      421 tggccgctcg tcgcgcgcgc gagctcgccg acgcgatcct ccccgccgaa ggggtcgacg
      481 tgttcgaccg ggtgctcgag agcccgctgg tggaggtcat cgtgtccacc cgtgatctgc
      541 cgaggctgat cgcgctggcg cggcagatgg ccgaggcgcc cgaggcggcg gtgcccgaga
      601 cggcccccgc ggcacccgcc agtgcgggag gccgagccgg gctgccgatc ccgttcgtgg
      661 cccccaccac cgaactcgag cggacgatcg cggaggtctg gcaggagctg ctgggcatcc
      721 agcccatcgg gacccaggac gacttcttcg aggccggcgg acactccctg ctcgcgaccc
      781 aggtgatgtc gcggctccac cagcggctcg gtgtcgacgt gccgctgcgc gtgctgttcg
      841 aggcgcggac cgtcgccgcg ttcgcggagc gggtggacga ggtgagccgc tcggccgccg
      901 agcgcgagga gatcgagctg tgacgcccgc cgccgaactg ctctcgcgcc tgcgcgccct
      961 cgacgtgaag atcagcgtcg aggggggcaa cctccgcgtg agtgccccga agggggcggt
     1021 cacctcggcg ctgcagcagg agctggcgag cctgaagccc gagatcatcg aggtgctcaa
     1081 gcagcgcggc gacgacgcca ccagcgggcc cacccggatg gcgcgggccg gcgacgtgcc
     1141 gctctccttc gtccagcagc gcctgtggtt cctcgaccag ctggagcccg ggacgtcgac
     1201 ctacaccatc gcggtgcgcc gccgcctgcg gggcccgctc gacgaatccg cgctgacccg
     1261 ggccctcacc gagctggtac ggcgccacga gtcgctgcgg accaccttcg tgacccgcaa
     1321 cggcgagccg cgccagcaga tcatgcccgc ggagccggtg gtaccggaga ccatcgatct
     1381 ggagagcgtg cccgccgcgg accgggaggc ggtggtggaa gagcacgtac acaaggaggt
     1441 gcagcggccc ttcagcctcg cgcgcgggcc cctcttccgt ccggtcctgt tgcgcctcgc
     1501 cccggacgag cacgagctga tcgcctcgat ccaccatatc gtggcggacg ggtggtcgct
     1561 gagcccgatc gcgcgggagc tcgagacgct ctaccaggcg tacaccaccg gcgccgagcc
     1621 cgccctgccc gagctgccgc tccagtacgc ggacttcgcg atctggcagc gccagtggct
     1681 caccggcgag gtgctggagg cgcaccgggc ctactggcgc gagcacctgg ccgcgctgcc
     1741 cgcgccgctc gagctgccca cggaccggcc gcgggcgcgc cagtcgagtc cggccgccgc
     1801 cagtcacgac ttcacgctac cgccggcgct ggccaacgcc ctgcgccagc tcggccgccg
     1861 cgagggcgtg acctccttca tgacgatgct cgccggcttc aaggcggtgc tcgcccgctg
     1921 caccggagcc accgacatcg tgctcggcac gccggtggcg aaccgtcatc acgtggagat
     1981 cgagcgcatc gtcggcttct tcgccaacac cctcgtgctc cgcacgagcc tggacgggga
     2041 cccgagcttc cgcgagctgc tgcgccgcgt gcgcgagatg agcctgggtg cctacgccca
     2101 ccaggatat
//
LOCUS       DATMCV010001037         3059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_124630, whole genome shotgun sequence.
ACCESSION   DATMCV010001037 DATMCV010000000
VERSION     DATMCV010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124630"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /gene="pyrH"
                     /locus_tag="VNU03_23790"
     CDS             <1..280
                     /gene="pyrH"
                     /locus_tag="VNU03_23790"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512115.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWP77567.1"
                     /translation="GAEAILKATKVDGIYTADPAKDKTAVRLPRVGYIEVLNRGLQVM
                     DTTAISLCMDNKLPIVVFDLTRKGNIRRIVTGEPVGSVVSTDAVPPKG"
     gene            284..844
                     /gene="frr"
                     /locus_tag="VNU03_23795"
     CDS             284..844
                     /gene="frr"
                     /locus_tag="VNU03_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWP77568.1"
                     /translation="MTQQAVQKDLETRMQAAVDVLAREFASVRTGRANAALLDGVRVE
                     AYGNMTPLNQVASVSVPDPRTILIQPWDATQLKEIEKGIVKSDLGLSPSNDGKVIRLT
                     MPTLTEERRKQLAKSVGKFAEDARVAIRNVRREANDKMKGLAKEKKISEDEERRGHDQ
                     IQKATDRFTAKIDELTKKKEQEVLTV"
     gene            1145..1849
                     /locus_tag="VNU03_23800"
     CDS             1145..1849
                     /locus_tag="VNU03_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HWP77569.1"
                     /translation="MAGPVPRHVAVIMDGNGRWATSRGLPRVAGHGEGVKAVRGIVRT
                     AGELGVQFLTLYAFSSENWQRPHQEVSTLMALLERSIERELPELMERNVRFRMIGRPE
                     GVPPRVGERIRRLIDTTAGNTGLTLLMAFNYGGRDELVDAVRTLAREVQRGALRPEDI
                     DEVRVSRALYTDGVPDPDLLIRTSGELRLSNFLLWQVAYTELWMTSTLWPDFGAREFY
                     LAVADFQQRDRRFGRV"
     gene            1849..2721
                     /locus_tag="VNU03_23805"
     CDS             1849..2721
                     /locus_tag="VNU03_23805"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWP77570.1"
                     /translation="MSRAEGVRIPRPVEAARPSRLAALVRRLLSTLVLLPLFVWMVVE
                     GPVWLFGAIMVLVGALGQWEFTGMFERAGVRTLRVVGLVGGTVLTASFALPISERVTL
                     TAVLFAIFGIGLLRVDVAGRAAWEPVAVTLFGVCYVNWLLGYTFWLRELDHGVEWVLL
                     LVSVTWLGETAAYVVGSALGRHKLAPSLSPRKTVEGAVAQLVMSGLAALGARAWFFPA
                     LSLESAIVVGLLLGVVGQAGDLIESAIKRSVGTKDTGRLIPGHGGMLDRVDSLLVNTP
                     VLFYYATYARSLGA"
     gene            2718..>3059
                     /locus_tag="VNU03_23810"
     CDS             2718..>3059
                     /locus_tag="VNU03_23810"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWP77571.1"
                     /translation="MKRLTVLGVTGSVGRRTLELVEHFPEEFRVEGMAARGSNPELIV
                     ELCRRHRPRALALSDAAAMDVVARALPTPRPELLAGPAGLVTLAREVEADVVLSAIVG
                     GAGLLPTMAAIQ"
BASE COUNT          430 a         1039 c         1100 g          490 t
ORIGIN      
        1 cggcgcggag gcgatcttga aggcgaccaa ggtggacggg atctacaccg ccgatccggc
       61 caaggacaag accgcggtgc gcctgccacg ggtcggctac atcgaggtgc tcaatcgcgg
      121 gctacaggtg atggacacca ccgccatctc cctgtgcatg gacaacaagc tgccgatcgt
      181 ggtcttcgat ctcacgcgca agggcaatat ccgacggata gtcacgggcg agccggtcgg
      241 atccgtcgtc tccaccgacg cggtcccccc gaaggggtga gccatgacgc aacaagcggt
      301 gcagaaggat ctcgagactc ggatgcaggc cgccgtcgac gtgctcgcgc gggagttcgc
      361 gagcgtgcgc accggccgcg ccaacgcggc gctcctggac ggggtgcgcg tcgaggccta
      421 cggcaacatg accccgctca accaggtggc gtcggtgtcg gtgcccgatc cgcgcaccat
      481 cctgatccag ccgtgggacg ccacccagct gaaggagatc gagaagggga tcgtcaagtc
      541 ggacctcgga ctctccccga gcaacgacgg caaggtgatc cggctcacga tgccgacgct
      601 gaccgaggag cggcgcaagc agctcgcgaa gtcggtgggc aagttcgcgg aggacgcgcg
      661 ggtcgcgatc cggaacgtgc ggcgcgaagc caacgacaag atgaaggggc tcgccaagga
      721 gaagaagatc tccgaggacg aggagcgtcg cggccacgac cagatccaga aggcgaccga
      781 tcgcttcacc gccaagatcg acgagctgac caagaagaag gagcaggaag tcctcacggt
      841 gtagggcggg caaccggtcg gcttactgcc gtttagttct tcaagctctt catctgcggg
      901 ggccccgaaa tggcccccgc actcccccag tccgcggacg ctcgttcccc cgtttggcgg
      961 acgctcgttt gtgtcctttg ccgagcacgc gttagtcgcc ggatttcgtc tgtatttcgg
     1021 cctttttact ggcaatccat ggggggctca cgtaacatcg tttgctatgg cggcgaaggc
     1081 cggggcctcg gtatcggtgg cggagttgtc gacgctcccg gaggacgacc tcaggcggcg
     1141 cgtcatggcg ggcccggtgc cgcgccacgt cgcggtcatc atggacggca acggccgctg
     1201 ggccaccagc cgggggcttc cgcgcgtcgc cggccacggc gagggcgtga aggcggtgcg
     1261 gggcatcgtg cgcaccgcgg gcgagcttgg cgtgcagttc ctcaccctct acgcgttctc
     1321 ctccgagaac tggcagcgcc cgcaccagga ggtctcgacg ctgatggcgc tgctcgagcg
     1381 ctccatcgag cgcgagctgc cggagctgat ggagcgcaac gtccgcttcc ggatgatcgg
     1441 gcggcccgag ggcgtgcccc cgcgggtggg cgagcgcatc cggcgcctca tcgacaccac
     1501 cgcgggcaac accggcctca ccctgctgat ggccttcaac tacggcggcc gcgacgagct
     1561 ggtcgacgcg gtgcggaccc tcgcgcgtga ggtccagcgc ggcgccctcc gcccggaaga
     1621 catcgacgag gtgcgcgtct cgcgcgcgct ctacaccgac ggggtgcccg acccggacct
     1681 cctcattcgc accagcggcg agctgcggct atcgaacttc ctgctgtggc aggtcgccta
     1741 caccgagctg tggatgacct cgacgctgtg gccggacttc ggagcgcgcg agttctacct
     1801 cgcggtggcc gacttccagc agcgggaccg tcgcttcggg agagtctgat gtcccgggcc
     1861 gagggcgttc gcatcccgcg cccggtcgag gcggcccgcc cgtctcgcct ggccgccctc
     1921 gtgcggcgcc tgctcagcac gctggtcctg ctcccgctgt tcgtgtggat ggtcgtggag
     1981 ggcccggtgt ggctcttcgg cgcgatcatg gtgctcgtgg gcgcgctcgg gcagtgggag
     2041 ttcaccggca tgttcgagcg ggccggcgtc cggacgctcc gggtggtggg cctggtgggc
     2101 gggacggtgc tgaccgcgag cttcgcgctg ccgatctcgg agcgggtcac gctcaccgcg
     2161 gtgctcttcg cgatcttcgg gatcgggctg ctgcgggtcg acgtcgcggg ccgcgccgcg
     2221 tgggagccgg tggcggtcac cctcttcggc gtctgctacg tgaactggct gctcggctac
     2281 accttctggc tgcgcgagct cgaccacggg gtggagtggg tcctgctcct ggtctcggtc
     2341 acgtggctgg gcgagaccgc cgcctacgtg gtgggctccg ccctcgggcg acacaagctc
     2401 gctccctccc tcagcccgcg caagacggtg gagggcgcgg tggcccagct cgtgatgtcg
     2461 gggctcgcgg ccctcggcgc gcgggcgtgg ttcttccccg cgctctccct cgagagcgcg
     2521 atcgtggtcg gactgctgct cggggtggtg gggcaggccg gcgacctcat cgagtccgcg
     2581 atcaagcgga gcgtcggcac caaggacacc ggtcggctca tccccggcca cggcggcatg
     2641 ctggatcgcg tggacagcct gctcgtcaac actccggtgc tcttctacta cgcaacctac
     2701 gcgaggtccc tgggcgcatg aagcggctga ccgtgcttgg tgtcaccggc tccgtggggc
     2761 ggcgcacgct ggagctggtc gagcatttcc cggaggagtt ccgggtcgag ggcatggccg
     2821 cgcgcggctc caaccccgag ctgatcgtgg agctctgccg gcgtcaccgg cctcgcgcgc
     2881 tcgccctgtc ggatgcggcc gcgatggacg tggtggcccg ggccctgccc accccgcgcc
     2941 ccgagctgct cgcggggccg gccggcctcg tgacgctcgc ccgcgaggtg gaggccgacg
     3001 tggtcctctc ggccatcgtg ggcggcgcgg gcctgctgcc gaccatggcc gccatccag
//
LOCUS       DATMCV010001038         6066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_256840, whole genome shotgun sequence.
ACCESSION   DATMCV010001038 DATMCV010000000
VERSION     DATMCV010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256840"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VNU03_23815"
     CDS             complement(<1..446)
                     /locus_tag="VNU03_23815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77572.1"
                     /translation="MDRRSALLVALASVLLAHLPAAPAPAAPARAAPAPATPALQVRL
                     VPPTARQGDTVVVFVAGTRGARDVEGSLGGHHLAFFPYGAEYAALAGVDLETKPGKLP
                     WRIGLVDAAGKPRKAAGALTVKAAGFPVQRLTLPGKMVDLDPEAERR"
     gene            complement(511..1461)
                     /locus_tag="VNU03_23820"
     CDS             complement(511..1461)
                     /locus_tag="VNU03_23820"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWP77573.1"
                     /translation="MSQYLTLHEEPGQLRRPVIILAFSGWNDAAESATTAARYLSQVW
                     SSRPLATIDPEDFYHFGLSRPYVRFKPGSRTEREVVWPATEFSVAQPTELERDIVVGV
                     AIEPHLKWRTYCAAVLELARRSGASLVLTLGALLAEVAHTRPVKLSGSSYDQELSARL
                     GLRPSRYEGPTGIVGVLNILCREASVPTASLWANVPHYVSGIENPRAALALVRRVLTL
                     LNAEADLTDLREAAEQFDQNLSSIVAQNEKISEYVRKLERKNPDDEGEPVEAVEEPEG
                     SADVPEVAQAMEGKDELPPSADLVAEIEQFLRQQQRPENS"
     gene            1606..1866
                     /locus_tag="VNU03_23825"
     CDS             1606..1866
                     /locus_tag="VNU03_23825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77574.1"
                     /translation="MRAWLSLASFLGGIVLLTSACATSEEWSEWRGHTTHFASGTHGV
                     FSLRNNKDGSNPRVARTDVDAARAENWWGKAITVSPEQIFQN"
     gene            1875..2585
                     /locus_tag="VNU03_23830"
     CDS             1875..2585
                     /locus_tag="VNU03_23830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77575.1"
                     /translation="MFRYDRTVRGGLLAALLFYGVSLTAQAYAAPADALMPIEQYTTA
                     KAKGLANAHRLQLIEFSEQIYNCMPWLAVYPGRLGFPKYKDSADDDRYLSMWIWVDQR
                     ENADFAAVPQDRRASAMFSRYGVDMLRRMAALSDVVDDSNLSGLSVVLSWLKPGTSRP
                     GQKPVNETLALFVDKATAADFLAKRVGSEEFVSRAKFNLFDGESLVGRVPLEVWEDSF
                     NTTYKVTNYDPPKGAKCQ"
     gene            2715..4613
                     /gene="ftsH"
                     /locus_tag="VNU03_23835"
     CDS             2715..4613
                     /gene="ftsH"
                     /locus_tag="VNU03_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWP77576.1"
                     /translation="MKLPNRQKTQFSLVYLFIALMVVFAIQSWIRAPRTVDIPMSQFL
                     TLVHEGKVLRVSLGEREIQGILKPGALPTPPAGPGDRLRQLLGAEQGPTVFTTARIPA
                     TDDYDVVRELQNAKVEFSGRIESTFWRDLISWLVPLVLMAGLWLFLMRRMGGGPTQAL
                     SFGRSKAKIYDRKELKTTFADVAGVDEAKAELMEVVDFLKNPKKYQRLGGRIPKGVLL
                     VGPPGTGKTLLARAVAGEADVPFFFLSGSEFVEMFVGVGASRVRDLFEQAKEKAPCIV
                     FIDELDAIGKTRAGNTGFLSGHDEREQTLNQLLAEMDGFDSSKGVIIMAATNRPEVLD
                     AALLRAGRFDRQVVVDKPDVRGREAILIVHARNVKLASSVDLHVLAARTPGMAGADLA
                     NIINEAALLAARKGKDAVDMADLEEAVDRVVGGLERKSRVLSEKERDIVAHHEIGHAL
                     VASSLPNADPVHKVTIIPRGVAALGATYQLPLEDRYLLTRSELEDRIAVLLGGRVAEE
                     IIYGEVSTGAHNDLERGTEIARLMVMQYGMSEALGPMTFGGGQQSMFLRGSGLPQERE
                     YSEESARRIDSETRAIIDRIYDRVRDLLTARKRVLVEAAAELKQKETLEGDRLRELLA
                     GEPVGAQR"
     gene            4610..>6066
                     /locus_tag="VNU03_23840"
     CDS             4610..>6066
                     /locus_tag="VNU03_23840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HWP77577.1"
                     /translation="MISLKRSTLGFVVLVTLVVGAGLGTWGAGAMDQIKPPVPGAGTL
                     GPLPSGTQPRIVPAALPIPSGSFAQVAESVGPAVVNINTVTRVSGRTPVEEFFGDEML
                     KRFFGETPERQQVQRSLGSGVIVDPSGIVLTNAHVVERATEIEVATADGKKHKAKVMG
                     ADRKTDLAVLKLQGGGPYPAANLGDSDRVKVGDWVLAIGSPFGLQQTVTAGIISAKGR
                     SIGQGPFDDFLQTDAAINPGNSGGPLVNMSGEVVGINSAILSRTGGNVGIGFSIPVNM
                     AKRIYTELAAKGKVTRGWLGVSIQPLTAELAKGFGLKEPNGVLISDVVSDSPAAQAGV
                     AAGDVIIEFDRKKVDSPQELQKVVAATAPGRAVPLKVWRANGEKMLEIKIGETPDESV
                     ALKPAGKGKGLLGMDVRPLTPELARQLNLRTAEGVIVFSVDEESAASEAGLQRGDVIR
                     EVNRQRVRSVQDFEKATKDVKDGDRVTVLLQRGPQS"
BASE COUNT          948 a         2132 c         2106 g          880 t
ORIGIN      
        1 cgccgctcgg cctccgggtc gaggtcgacc atcttgccgg gcagggtcag ccgctgcacc
       61 gggaatcccg ccgccttgac ggtgagcgcg cctgccgcct tgcgcggctt gccggccgcg
      121 tccaccaggc cgatccgcca cggcagcttg ccgggcttgg tctcgaggtc cacgccggcc
      181 agggcggcgt actccgcccc gtaggggaag aaggccaggt ggtgcccgcc caggctccct
      241 tccacgtcgc gcgcgccccg ggtcccggcc acgaagacca ccaccgtgtc cccctgccgg
      301 gcggtcggcg ggacgagacg tacctggagc gcgggggtcg caggagccgg agccgcgcga
      361 gccggcgccg cgggcgccgg cgccgcaggc aagtgcgcca gcaggacgct ggcgagggca
      421 acgagcaggg cggaccggcg atccatcggg aagatcttac gcttccccgc cccggaaaca
      481 cgcactccgc cccccttccg gggagcgggc tcaggaattc tcgggacgct gctgctggcg
      541 gaggaactgc tcgatctcgg ccaccaggtc ggcggagggc ggcagctcgt ccttgccctc
      601 catggcctgg gcgacctccg ggacgtcggc cgagccctcg ggctcctcga cggcctcgac
      661 cggctcgccc tcgtcgtcgg gattcttgcg ctcgagcttc cgcacgtact cggagatctt
      721 ctcgttctgc gccacgatcg agctgagatt ctgatcgaac tgctcggccg cttcgcgcag
      781 gtcggtcagg tccgcctcgg cgttcagcag ggtcagcacc cgccggacga gggccagcgc
      841 ggcgcggggg ttctcgatgc cggagacgta gtgcggcacg ttcgcccaca ggctggccgt
      901 cggcacgctc gcctcgcggc acaggatgtt gaggaccccc acgattccgg tcggtccttc
      961 gtagcgcgag ggcctgagcc ccaggcgcgc ggacagctcc tgatcgtagg agctcccgga
     1021 cagcttgacc ggccgggtgt gggcgacctc ggccagcagc gcgcccagcg tgagtaccag
     1081 cgaggcgccg gagcgccggg ccagctcgag gaccgcggcg cagtaggtgc gccacttgag
     1141 atgcggctcg atggccaccc cgaccacgat gtcgcgctcg agctcggtcg gctgggccac
     1201 cgagaactcg gtcgcaggcc agaccacctc gcgctcggtc cgcgagcccg gcttgaagcg
     1261 gacatagggc cgggacaggc cgaagtggta aaaatcttcc gggtcgatgg tggccagcgg
     1321 ccgggacgac cagacctggc tcaggtagcg tgccgcggtg gtggccgatt cggccgcgtc
     1381 gttccagccc gagaacgcca ggatgatgac gggccggcgc aactgcccgg gctcctcgtg
     1441 cagcgtcagg tactggctca tcggtggcgc tgcctcctcc agggagcatg cctgaggggt
     1501 cccctccagg caaatgaaag ttgacctctc ccgaagccct ccgtatcatt acgatccgtg
     1561 caccggaagc ggaagactgg agtgccctca aggaggcaag ttcccatgcg cgcatggcta
     1621 tcgttggctt cgttcctcgg aggcatcgtg ctgctgacct cggcgtgcgc cacgtcggag
     1681 gagtggtcgg agtggcgggg ccacaccact cacttcgcct cgggtacaca cggggtcttc
     1741 tcgctgcgga acaacaagga cggctcgaat ccgcgcgtgg cgcgcacgga cgtcgacgcg
     1801 gcgcgggcgg agaactggtg gggcaaggcc atcaccgtga gcccggagca gatcttccag
     1861 aactagtccc gctcatgttc cggtacgacc gcaccgtgcg gggcggcctc ctggccgccc
     1921 tgctgttcta cggggtgtcc ctgacggccc aggcctacgc ggcccccgcg gatgccctga
     1981 tgccgatcga gcagtacacg actgccaagg ccaagggcct ggccaatgcc caccgtctcc
     2041 agctgatcga gttctccgag cagatctaca actgcatgcc gtggctcgcc gtctacccgg
     2101 gacgcctcgg cttcccgaag tacaaggaca gtgccgacga cgaccgctat ctgtcgatgt
     2161 ggatctgggt cgatcagcgc gagaacgcgg acttcgccgc cgtgccccag gaccgtcgcg
     2221 cctccgcgat gttctcgcgc tatggcgtcg acatgctgcg gcgcatggcc gcgctgtcgg
     2281 acgtcgtcga cgacagcaac ctatccgggc tctcggtggt gctgtcgtgg ctcaagccgg
     2341 gcaccagtcg ccccggccag aagccggtga acgagaccct cgcgctgttc gtcgacaagg
     2401 ccaccgcggc cgacttcctg gccaagcggg tggggtccga ggagttcgtg agccgggcca
     2461 agttcaatct gttcgacggc gagagcctgg tcgggcgcgt gcctctcgag gtgtgggagg
     2521 actctttcaa cacgacttac aaggtcacca actacgaccc ccccaagggg gccaaatgcc
     2581 agtagctcgt ccggcctgac ccaggtccgg ccgctgttcc ccgctgagcc gtcgcgaccc
     2641 gccgattctc catcgcgggc cgccggtttg acacccccca ggggttccgc tagcatcgaa
     2701 tccgagcccc cgcgatgaaa ttgcccaacc ggcagaagac ccagttttcc ctcgtctatc
     2761 tcttcatcgc gctgatggtg gtgttcgcca tccagagctg gatccgggcc ccccggaccg
     2821 tcgacatccc catgagccag ttccttaccc tggtgcacga gggcaaggtc ctgcgggtct
     2881 ccctgggcga gcgcgagatc cagggcatcc tcaagcccgg cgccctgccc acgcccccgg
     2941 ccggacccgg cgatcggctg cgccagctcc tgggcgccga gcaggggccg accgtattca
     3001 ccaccgcgcg catccccgcc accgacgact acgacgtcgt ccgcgagctg cagaacgcga
     3061 aggtggagtt ctccggccgc atcgagtcca cgttctggcg cgacctgatc tcctggctgg
     3121 tgccgctggt cctgatggcc ggcctctggc tcttcctgat gcggcggatg ggaggcgggc
     3181 ccacgcaggc cctctccttc gggcgctcca aggccaagat ctacgatcgc aaggagctga
     3241 agaccacctt cgcggacgtg gccggcgtcg acgaggccaa ggccgagctg atggaggtgg
     3301 tcgacttcct gaagaacccg aagaagtacc agcgcctcgg cggccgcatc ccgaagggcg
     3361 tgctcctggt gggcccgccg ggcaccggca agaccctgct ggcccgcgcg gtggcgggcg
     3421 aggccgacgt gccgttcttc ttcctctccg gctccgagtt cgtggagatg ttcgtgggcg
     3481 tgggcgcctc gcgggtgcgc gacctcttcg agcaggccaa ggagaaggcg ccctgcatcg
     3541 tgttcatcga cgagctcgac gccatcggca agacccgcgc gggcaacacc ggcttcctct
     3601 ccggccacga cgagcgggag cagaccctca accagctcct cgcggagatg gacggcttcg
     3661 actcgtccaa gggcgtgatc atcatggcgg ccaccaaccg gcccgaggtg ctcgacgcgg
     3721 cgctgctgcg ggccggccgc ttcgaccgcc aggtggtggt ggacaagccc gacgtgcggg
     3781 gccgcgaagc gatcctgatc gtgcacgccc gcaacgtgaa gctcgcctcg agcgtggacc
     3841 tgcacgtgct ggccgcgcgc acccccggca tggccggcgc ggacctggcc aacatcatca
     3901 acgaggccgc gctcctggcc gcgcgcaagg gcaaggacgc ggtcgacatg gccgacctcg
     3961 aggaggcggt ggaccgcgtg gtgggcggcc tcgagcgcaa gagccgcgtg ctctccgaga
     4021 aagagcgcga catcgtcgcg caccacgaga tcggccacgc gctggtcgcc tcctcgctgc
     4081 ccaacgcgga cccggtgcac aaggtgacca tcatcccgcg cggggtcgcc gccctgggcg
     4141 ccacctacca gctgccgctg gaggaccgct acctcctcac ccgcagcgag ctcgaggacc
     4201 ggatcgcggt cctgctcggg ggccgggtgg ccgaggagat catctacggc gaggtctcga
     4261 ccggggccca caacgacctc gagcggggca ccgagattgc ccggctcatg gtcatgcagt
     4321 acggcatgtc cgaggcgctc gggcccatga ccttcggcgg ggggcagcag tcgatgttcc
     4381 tccgaggctc gggcctgccg caggagcgtg aatacagcga ggagagcgcc cgtcgcatcg
     4441 actccgagac gcgcgccatc atcgatcgca tctacgaccg cgttcgcgat ctcctcaccg
     4501 cccgcaagcg ggtgctggtg gaggccgccg ccgagctgaa acagaaagag acgctggagg
     4561 gcgaccggct gcgcgagctg ctggccggcg agcccgtggg ggcacagaga tgatctctct
     4621 caagcgcagc acgttggggt tcgtggtcct cgtgacgctc gtggtcggcg cggggctcgg
     4681 cacctgggga gccggcgcca tggaccagat caagccgccc gtgcccggcg cgggcacact
     4741 gggtcccctg ccctcgggca cccagcctcg catcgtgccg gcagccctgc ccatcccgtc
     4801 gggcagcttc gcccaggtgg cggagagcgt ggggccggcg gtggtcaaca tcaacaccgt
     4861 gacccgggtg agcggacgga cgccggtgga agagttcttc ggcgacgaga tgctcaagcg
     4921 gttcttcggc gagacccccg agcggcagca ggtccagcgg agcctcggct cgggcgtgat
     4981 cgtcgacccc tccggcatcg tgctcaccaa cgcccacgtg gtcgagcgcg ccaccgagat
     5041 cgaggtcgcc accgccgacg gcaagaagca caaggccaag gtgatgggcg cggaccgcaa
     5101 gaccgacctg gccgtgctca agctccaggg cggcgggccc tatcccgccg cgaacctggg
     5161 cgactccgac cgggtcaagg tcggcgactg ggtcctcgcc atcggctcgc cgttcggcct
     5221 ccagcagacg gtgaccgccg ggatcatcag cgccaagggc cgctcgatcg gccagggtcc
     5281 cttcgacgac ttcctccaga ccgacgcggc catcaatccg gggaactcgg gcggcccgct
     5341 cgtgaacatg tcgggcgagg tggtcggcat caacagcgcc atcctctcgc gcaccggcgg
     5401 caacgtgggc atcggcttct cgatcccggt gaacatggcc aagcggatct acaccgagct
     5461 ggccgccaag ggcaaggtca cccgcggctg gctcggggtg tcgatccagc cgctcaccgc
     5521 cgagctggcc aagggcttcg ggctcaagga gcccaacggg gtgctgatct ccgacgtggt
     5581 ctctgacagc ccggccgccc aggcgggcgt cgccgccggc gacgtcatca tcgagttcga
     5641 ccggaagaag gtcgactccc cccaggagct gcagaaggtg gtggccgcga ccgcgccggg
     5701 gcgcgcggtg ccgctcaagg tctggcgcgc caacggcgag aagatgctcg agatcaagat
     5761 cggcgagacg ccggacgaga gcgtggcgct caagcccgcc ggcaagggca agggcctcct
     5821 cggcatggac gtgcggccgc tcacgcccga gctggcccgc cagctgaacc tgcgtaccgc
     5881 ggagggcgtc atcgtgttct cggtggacga ggagagcgcg gcctccgagg ccggcctgca
     5941 gcgcggggac gtcatccgcg aggtgaaccg gcagcgggtg cgcagcgtgc aggacttcga
     6001 gaaggcgacc aaggacgtca aggatggcga ccgcgtgacc gtgctgctcc agcgggggcc
     6061 gcaatc
//
LOCUS       DATMCV010001039         4481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_789543, whole genome shotgun sequence.
ACCESSION   DATMCV010001039 DATMCV010000000
VERSION     DATMCV010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789543"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VNU03_23845"
     CDS             <1..1047
                     /locus_tag="VNU03_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77578.1"
                     /translation="RLSEVAGGAEFPMEASEVAVSQWVYEHGQVAGQGTDTLPGAAGL
                     YVPLLGSRGAVGVLGLRPTDPRSLQAPEQLHQVETFASQTALAIERAQLAAAAEQAQV
                     RAETERLRNSLLSSVSHDLRTPLASITGAASTLLENEARLDGATRRDLLEALHEEADR
                     LNRLVQNLLEMTRLEAGALVPHTAWHSVEEVVGAALGRFGKSLAERPVTTRIPAELPL
                     VPMDDVLIEQVLINLIDNAIKYTPAGTPIEVSAEDTGAAVVVEVADRGPGLRPGEERL
                     IFEKFHRTDPAPSVRGAGLGLAICQGIIQAHGGRIWAENRPGGGVALRFTLPLKDMPP
                     TPSESPAQAESDVP"
     gene            1037..1756
                     /locus_tag="VNU03_23850"
     CDS             1037..1756
                     /locus_tag="VNU03_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77579.1"
                     /translation="MSPDLPAPEASPPSPVVVLIEDEPQIRRFLRATLGSSGYRLYEA
                     TSGEDGLVEAATRQPDVVILDLGLPDIDGLQVIRRLREWSKVPIIVLSARGQEGDKVA
                     ALDAGADDYVSKPFAVGELLARLRVALRHAAVGESGEPTFTVAGLRVDQVKRLVQMDG
                     KDVHLTPIEYRLLTTLVRHAGRVLSQRQLLKEVWGPAHTDQAHYLRVYMGTLRRKLEQ
                     DPARPRYLLTEPGVGYRLAAE"
     gene            1871..2077
                     /locus_tag="VNU03_23855"
     CDS             1871..2077
                     /locus_tag="VNU03_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77580.1"
                     /translation="MNPQMEEQVARQRLDEARAEAARWALLRQLRPARHPVRVALGFA
                     LIKVGHWVAGRAPRRAGQPRRATA"
     gene            2092..3093
                     /locus_tag="VNU03_23860"
     CDS             2092..3093
                     /locus_tag="VNU03_23860"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWP77581.1"
                     /translation="MARESSQIVPPSGLRVNRKSAVPVHVQLQTQVRHLIDTGALKPG
                     MQMPTVRQLAGFLRINRNTVARALADLSQDGYLESHQGRGTFVADRPPMREGRAARSL
                     ERLVREALERARRLGFTHEELAATIAASAPPARGARRARVRVLLVECNHAELSRYREQ
                     LEEELSLTVDRLLVEELAARMREPGFFKDYRVVITTFFHIHEVKQALPLDGPPAVALL
                     SESNISSLLRLTELPEGTSVGLVCNTLRGSQNILSSVQSAGLSHVVPILASTDDPWSI
                     DRMLEKTRIVVCSEQAADQIRERFPHDVEVIFAYRRLDNGGIEMLRDLLAQVEGERA"
     gene            3090..3503
                     /locus_tag="VNU03_23865"
     CDS             3090..3503
                     /locus_tag="VNU03_23865"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWP77582.1"
                     /translation="MTAADAMTPGPLTVTPRTSVAAALRLIRDNRIAHLPVVDRGRIV
                     GVVRQERLLALTPSEATTLSRHELLDALALLTVERAVEPALTLAARTPLAEAARHLLE
                     RGAHEALVVDEDRLVGILTHSDLLRALIAKPEAVA"
     gene            3500..4138
                     /locus_tag="VNU03_23870"
     CDS             3500..4138
                     /locus_tag="VNU03_23870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWP77583.1"
                     /translation="MGTGPAVCVFDLDHTLVDSPLDLRAVGREMEAFLRARNVAVPER
                     ELRWSGAELFDLVRREALHLEAELLAIPVAHERRAMAQATLIPFAREAVAAMKQLGFA
                     TAIWTNNDRMVADFVLSRFDLLPHLDLVVTRDEVRRLKPDPYGLRVVRERWPDASRIV
                     VVGDSWVDGLAAQAAGVPFIAYRPDVAQMEERGVVIAARIHSLLDLPAALAE"
     gene            4294..>4481
                     /locus_tag="VNU03_23875"
     CDS             4294..>4481
                     /locus_tag="VNU03_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWP77584.1"
                     /translation="MRPRSLDEVVGQPQLTEPGRPLRRALEQGALHSMILWGPPGTGK
                     TTLARLLADVAGARFVPFS"
BASE COUNT          628 a         1659 c         1586 g          608 t
ORIGIN      
        1 cggctgagcg aggtcgccgg gggcgccgag ttcccgatgg aggcgagcga ggtggcggtg
       61 agccagtggg tgtacgagca cgggcaggtc gcaggccagg gcacggacac cctgccgggc
      121 gccgccggcc tctacgtgcc cctgctgggc tcgcggggcg cggtcggcgt gctggggctc
      181 cggccgaccg acccgcgctc gctccaggcg ccggagcagc tccaccaggt cgagacgttc
      241 gcgagccaga ccgcgctcgc catcgagcgc gcgcagctcg cggcggcggc cgaacaggcg
      301 caggtgcgcg ccgagaccga gcggctgcgc aactccctgc tcagctcggt ctctcacgac
      361 ctgcggacac cgctggcctc gatcaccggg gcggccagca cgctgctgga gaacgaggcg
      421 cgcctggacg gcgcgacccg gcgcgatctg ctggaggcct tgcacgagga ggccgaccgg
      481 ctgaaccggc tcgtccagaa cctgctcgag atgacccgcc tcgaggcggg ggcgctcgtg
      541 ccccacaccg cgtggcactc ggtcgaggaa gtggtcgggg cggcgctcgg gcgtttcggc
      601 aagtcgctcg cggagcggcc agtgaccacc cggatcccgg ccgagctgcc cctcgtcccc
      661 atggacgacg tgctcatcga gcaggtgctg atcaacctga tcgacaacgc gatcaagtac
      721 acgccggccg ggacgccgat cgaggtgagc gcggaggaca ccggcgccgc ggtggtggtc
      781 gaggtcgccg accggggacc aggactgcgg ccgggcgagg agcgcctcat cttcgagaag
      841 ttccaccgga cggaccccgc cccctcggtg cggggcgccg ggctcggcct ggccatctgc
      901 caggggatca tccaggcgca cggcggacgg atctgggcgg agaaccgccc cggtggcggc
      961 gtggccctcc gcttcaccct gcccttgaag gacatgccgc caacgccgtc agagtccccc
     1021 gcgcaggccg aatcagatgt cccctgacct gccggctccc gaggccagcc cgccttcccc
     1081 ggtcgtggtg ctgatcgagg acgagcccca gatccgtcgc ttcctccggg ccaccctcgg
     1141 cagcagcggc tatcgcctgt acgaggccac gtccggcgag gacggtctgg tcgaggcggc
     1201 gacccgccag ccggatgtgg tcatcctcga cctgggtctg cccgacatcg acggcctgca
     1261 ggtgatccgg cggctgcggg agtggagcaa ggtcccgatc atcgtgctct cggcccgcgg
     1321 gcaggagggc gacaaggtcg ccgcactgga cgcgggggcc gacgactacg tgagcaagcc
     1381 gttcgcggtg ggagagcttc tcgcgcggct gcgggtcgcc ctccgccatg ccgccgtcgg
     1441 cgagtccggc gagccgacct tcacggtggc cgggctgcga gtggaccagg tcaagcgact
     1501 ggtccagatg gacggcaagg acgtccacct gacccccatc gagtaccggc tgctgaccac
     1561 cctggtccgg cacgccggcc gggtgctgag ccagcgacag ctattaaagg aggtgtgggg
     1621 gccggcccac accgaccagg cccactacct ccgggtctac atggggactc tccgacggaa
     1681 actcgagcag gaccccgccc gtccccggta cctcctgacc gagcccggcg tggggtatcg
     1741 gctcgccgcc gaataacttg acacggggca tcacactgtc atagtgtctt agtgtactag
     1801 gacactaagc aggccggtcc agtacccagg acgaactgtc ctagggacac ctcgaagagg
     1861 aggcagtgtg atgaacccgc agatggaaga gcaggtggcc aggcagcggc tggacgaggc
     1921 tcgggccgag gcggcccgat gggccctcct ccgccagctt cggccggcgc ggcacccggt
     1981 gcgcgtggcg ctcggcttcg ccctgatcaa ggtcggccac tgggtggccg ggcgggcccc
     2041 ccgacgggcg ggccagccgc gacgagccac ggcctaggga ggagccagca cgtggcgcgg
     2101 gaatcctcgc agatcgtccc gccatcgggg ctccgggtga accggaagag cgcggttccc
     2161 gtccacgtgc agctccagac ccaggtccgt catctcatcg acaccggcgc gctgaagccg
     2221 ggcatgcaga tgcccaccgt gcgccagctg gccggctttc tccgcatcaa ccgcaacacg
     2281 gtcgcccggg ccctggccga cctctcccag gacggctatc tcgagagcca ccagggccga
     2341 ggcaccttcg tggccgatcg cccgccgatg cgcgagggcc gcgcggcccg cagcctcgag
     2401 cggctcgtgc gggaggcgct cgagcgcgcg cgccggctcg ggttcaccca cgaggagctc
     2461 gccgcgacca tcgcggccag cgcgcccccg gcgcggggcg cccggcgggc ccgggtgcgt
     2521 gtgctcctgg tggagtgcaa ccacgccgag ctctcccgct accgcgagca gctcgaggag
     2581 gagctgtcgc tcacggtgga tcgcctgctg gtcgaggagc tggcggcccg gatgcgcgag
     2641 cccggcttct tcaaggacta ccgggtcgtg atcacgacct tcttccacat ccacgaggtc
     2701 aagcaggcgc tgccgctcga cggtccgccc gcggtggccc tgctctcgga gtcgaacatc
     2761 tcctccctgc tgcgcctgac cgagctgccc gagggcacca gcgtcgggct cgtctgcaac
     2821 accctccgcg gcagccagaa catcctgagc tcggtgcagt cggccgggct ctcgcacgtg
     2881 gtgccgatcc tggcctcgac cgacgatccc tggtccatcg accggatgct cgagaaaacg
     2941 cgcattgtgg tctgctccga gcaggccgcg gaccagatcc gcgagcgctt cccgcacgac
     3001 gtcgaggtga tcttcgccta tcggcggctc gacaacggcg gcatcgagat gctccgggac
     3061 ctgctggccc aggtcgaggg ggagcgcgcg tgaccgccgc cgacgcgatg acgccgggcc
     3121 cgctcaccgt gaccccgcgc acgagcgtgg ccgcggccct gcgcctcatc cgcgacaacc
     3181 ggatcgccca cctgccggtg gtggaccgcg ggcgcatcgt gggcgtggtg cgccaggagc
     3241 ggctgctggc cctgacgccc tccgaggcga ccacgctgtc gcgccacgag ctgctcgacg
     3301 cgctggcgct cctcaccgtc gagcgcgcgg tggagcccgc cctgaccctg gccgcgcgca
     3361 ccccgctcgc ggaggccgcg cggcacctgc tcgagcgcgg cgcccacgag gcgctggtgg
     3421 tcgatgagga ccgcctggtc ggcatcctca cccactccga tctcctgcgg gccctgatcg
     3481 cgaaaccgga ggccgtggca taggcaccgg ccccgcggtc tgcgtcttcg acctcgacca
     3541 caccctggtc gactcccccc tcgatctccg agcggtgggg cgcgagatgg aagcgttcct
     3601 gcgcgcccgc aacgtcgcgg tgcccgagcg cgagctgcgc tggtcggggg ccgagctctt
     3661 cgacctggtc cggcgcgagg cgctccacct ggaggccgag ctgctcgcga tcccggtggc
     3721 ccacgagcgg cgggccatgg cccaggccac cctgatcccg ttcgcgcgcg aggcggtcgc
     3781 ggccatgaag cagcttggct tcgcgaccgc gatctggacc aacaacgacc gcatggtcgc
     3841 cgacttcgtc ctctcccgct tcgacctgct cccgcacctg gatctggtgg tgacgcgcga
     3901 cgaggtgcgc cggctcaagc cggatccgta cggcctccgc gtggtgcgcg agcgctggcc
     3961 cgacgcctcc cgcatcgtgg tcgtcggcga ctcctgggtc gacgggctcg ccgcgcaggc
     4021 cgccggcgtg cccttcatcg cctaccgccc cgacgtggcc cagatggaag agcgcggcgt
     4081 ggtcatcgcg gcgcggatcc actcgttgct cgatctgccg gcggcgctgg ccgagtagac
     4141 gctcctcacc cctcgaagcc cctcacccgt agaagcccct caccctgccc tctccccggg
     4201 ggggagaggg agaatgggta tactgcgcgg gtggatctct tcgattcgcc cccggcggcc
     4261 gcgcccaagg cgcccgcgcc gctcgccgat cgcatgcgcc cgcgaagcct cgacgaggtg
     4321 gtcggacagc cacagctgac cgagccgggc cggccattgc gccgggccct cgagcagggc
     4381 gcgctgcact cgatgatcct gtggggcccg cccggcaccg gcaagaccac gctcgcgcgc
     4441 ctgctcgccg acgtggcggg ggcccgcttc gtgccgttct c
//
LOCUS       DATMCV010001040         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_191248, whole genome shotgun sequence.
ACCESSION   DATMCV010001040 DATMCV010000000
VERSION     DATMCV010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191248"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VNU03_23880"
     CDS             <1..524
                     /locus_tag="VNU03_23880"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWP77585.1"
                     /translation="RVLDVLAREGVRAAFFLIGQRARRAPEIARRIAGAGHDLGNHTW
                     SHRSLWRCGPAHTQREIQDGHTAIADAAGEPPRFFRPPWGKTNLAMFGVARQLDTPCV
                     FWTVQPEGRRPVDPAEQARRGIARARAGAIYDLHDADGVPGAGARLVEYLPTLVAGLR
                     AQGYALVPLRDLL"
     gene            566..2257
                     /gene="ilvD"
                     /locus_tag="VNU03_23885"
     CDS             566..2257
                     /gene="ilvD"
                     /locus_tag="VNU03_23885"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWP77586.1"
                     /translation="MAVDPRKRSRVLLEGTDRAAARAMMKAVGFSDQDLARPQIGVAH
                     SWIGTMPCNWNHRKLAEKVMQGIRAAGGTPIEVNTISITDGITMGTEGMKGSLISREV
                     VADSVELVARSHMFDGIVTISGCDKTIPAMTMVLGRLDIPSLMLYCGSIMFGRCAGTE
                     GPFAGRNLTIQDVFEAIGAYNAGKIPLEQFKDVEDHACPGAGACGGQFTANTMATAYE
                     MLGMSPMGWNDIPATDPRKEEVAFESGKLVMDLVRQGLTPRKIVTRKSFENSIAGVMA
                     TGGSTNAVLHLMATARDFGVKLAIEDFDRISRKTPVVADLKPWGTYTAPEMYDAGGMA
                     VVGKRLLEARLLHGSEKTVTGRTIAEEVKAAPEPAGQQVIKPLAKALKAEGGIAILRG
                     NLAPGGCVIKLSGQKKYSHRGPARVFEVEEDAFLAIKKGKIKPNDVIVIRYEGPRGGP
                     GMREMLHVSGALQGAGLGESVALMTDGRFSGATHGFMIGHIVPEAAVRGPIAAVRNGD
                     TISIDVKKRRLDLEVPAGEIKKRLAKWKAPKPRFTIGVFAKYAAGVADASVGAYT"
     gene            complement(2342..>3015)
                     /locus_tag="VNU03_23890"
     CDS             complement(2342..>3015)
                     /locus_tag="VNU03_23890"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP77587.1"
                     /translation="HITEVARMLEIGRVVVPRVASVLSAWGMLATDLRYEFVRTHVGE
                     AGQVGAGRLRRLFAEMEAEGRRRLGKSFAGRVDVRGSVDMRYGEQTFEIQVPLDGVDV
                     EAKDLMEQVVDRFHRRHEELYTYSAPGQDVVLVNVRAAVIGRLPALPAEQSRPVSAPA
                     APRSRRVYLERWMEIPVYDMDGLADGQEVKGPAIFESATTTVLIREREHARVTAEGWL
                     DIRLG"
BASE COUNT          480 a         1103 c         1035 g          397 t
ORIGIN      
        1 cgcgcgtgct cgacgtcctc gcccgcgaag gcgtccgggc cgcgttcttc ctcatcggac
       61 agcgcgcgcg gcgagccccc gagatcgccc gccgcatcgc cggggccggc cacgacctgg
      121 gcaaccacac ctggagccac cggagcctct ggcgctgcgg ccccgcccac acccagcgcg
      181 agatccagga cggccacacc gccatcgcgg acgcggcggg cgagccgccc cgcttcttcc
      241 ggcccccctg gggcaagacc aacctggcca tgttcggggt ggcccggcag ctcgacacgc
      301 cctgtgtctt ctggacggtt cagccggagg gccggcgccc ggtggatccg gccgagcagg
      361 cgcggcgggg gatcgcgcgg gcccgcgcgg gcgcgatcta cgacctgcac gacgccgacg
      421 gcgtgcccgg cgcgggcgcc cggctggtgg agtacctgcc caccctcgtc gcgggcctcc
      481 gcgcgcaggg ctacgcgctg gtcccgctcc gcgacctgct gtagaatcgc gccaacccga
      541 gcgccccacg acaaaggagt ctgcgatggc cgtcgatccc cgcaagcgaa gccgcgtcct
      601 tctcgaaggc accgaccgtg ccgccgcccg cgccatgatg aaggcggtcg gcttcagcga
      661 ccaggatctc gcgcgccccc agatcggggt ggcccactcc tggatcggga ccatgccctg
      721 caactggaac caccggaagc tggccgagaa ggtcatgcag ggcatccggg cggcgggcgg
      781 cacgccgatc gaggtcaaca ccatctcgat caccgacggc atcaccatgg gcaccgaggg
      841 catgaagggt tctctcatca gccgagaggt cgtggccgac tcggtggagc tcgtcgcgcg
      901 cagccacatg ttcgacggca tcgtcacgat ctccggctgc gacaagacga tcccggccat
      961 gaccatggtg ctgggccgcc tcgacatccc gagcctcatg ctctactgcg gctcgatcat
     1021 gttcgggcgc tgcgccggca ccgaggggcc gttcgcgggc cgcaacctca ccatccagga
     1081 cgtgttcgag gcgatcgggg cctacaacgc gggcaagatc ccgctcgagc agttcaagga
     1141 cgtggaggac cacgcctgtc cgggcgcggg cgcctgcggc ggccagttca ccgccaacac
     1201 catggccacc gcctacgaga tgctcggcat gtcgccgatg ggatggaacg acattcccgc
     1261 caccgacccc cggaaggaag aggtcgcctt cgagtcgggc aagctcgtga tggatctcgt
     1321 gcggcagggt ctgaccccgc gcaagatcgt cacccgcaag agcttcgaga actccatcgc
     1381 cggcgtgatg gccaccggag gctccaccaa cgcggtgctg cacctcatgg cgaccgcgcg
     1441 ggacttcggg gtcaagctcg cgatcgagga cttcgaccgc atctcgcgca agactccggt
     1501 ggtcgccgac ctgaagccgt ggggcaccta caccgcgccc gagatgtacg acgcgggcgg
     1561 gatggcggtg gtgggcaagc gcctcctcga ggcgcgcctg ctccacggga gcgagaagac
     1621 ggtcaccggc cgcaccatcg ccgaggaggt gaaggctgcc cccgagccgg cgggccagca
     1681 ggtgatcaag ccactcgcaa aggcgctgaa ggccgagggc ggcatcgcga tcctgcgggg
     1741 caacctggcc cccggcgggt gcgtgatcaa gctctccggc cagaagaagt actcgcaccg
     1801 cgggcccgcg cgcgtcttcg aggtcgagga ggacgccttc ctggcgatca agaagggcaa
     1861 gatcaagccc aacgacgtga tcgtgatccg ctacgagggt cccaggggcg ggcccggcat
     1921 gcgcgagatg ctgcacgtct cgggcgcgct gcagggggcc ggtctcggcg agagcgtggc
     1981 gctcatgacc gacggccgct tctcgggcgc cacccacggc ttcatgatcg gccacatcgt
     2041 gcccgaggcg gcggtgcgcg gcccgatcgc ggcggtgcgg aacggcgaca cgatctcgat
     2101 cgacgtgaag aagcggcggc tcgatctcga ggtgcccgcg ggcgagatca agaagcgcct
     2161 ggccaagtgg aaggcgccca agccgcgctt caccatcggc gtcttcgcca agtacgcggc
     2221 cggcgtggcc gacgcctcgg tcggcgccta tacataggtc gttcccagct catccccctc
     2281 tcccctcagg ggagagggca gggtgagggg tggtgtaggt gaggggcggc gaccccgaag
     2341 gctaccccag ccggatatcc agccacccct ccgcggtgac ccgcgcgtgc tctcgctcgc
     2401 ggatcaacac ggtcgtggtg gccgactcga agatcgcggg gcccttcacc tcctggccat
     2461 ccgcgagccc gtccatgtca tacaccggga tctccatcca ccgctcgagg tagacgcgac
     2521 gactccgggg cgcggccggc gcgctcaccg gtcgcgattg ctcggcgggc agcgcgggca
     2581 ggcgcccgat caccgcggcg cgcacgttca ccagcaccac gtcctgaccg ggcgcgctgt
     2641 aggtgtagag ctcctcgtga cgccggtgga agcggtccac gacctgctcc atgaggtcct
     2701 tcgcctccac gtcgaccccg tcgagcggca cctggatctc gaaggtctgc tcgccgtagc
     2761 gcatgtccac cgagccgcgc acgtcaaccc ggcccgcgaa cgacttgccg agccggcgcc
     2821 gcccctccgc ctccatctcc gcgaagagcc gccgcaggcg cccggcgccg acctggccgg
     2881 cctcgcccac gtgggtgcgc acgaactcgt agcgcaggtc ggtggccagc atgccccacg
     2941 ccgagaggac cgaggcgacc cgcggcacca cgacccggcc gatctcgagc atgcgcgcga
     3001 cctcggtgat gtgga
//
LOCUS       DATMCV010001041         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_257007, whole genome shotgun sequence.
ACCESSION   DATMCV010001041 DATMCV010000000
VERSION     DATMCV010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257007"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VNU03_23895"
     CDS             complement(<1..429)
                     /locus_tag="VNU03_23895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77588.1"
                     /translation="MSATRLDWGKIFRVTATVGFVSSAALHLLTFTPFPPAYAAAVAL
                     VLLAGAFLLLAAMIVRLRESGAPARGRGTVRMVEWRVLLAQIPRGPKHAAVAVIAYVL
                     MNLGLCLLLGDQGAASVRLLSGHLLLFYLIPFMYFRFVEPR"
     gene            640..2073
                     /locus_tag="VNU03_23900"
     CDS             640..2073
                     /locus_tag="VNU03_23900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWP77589.1"
                     /translation="MARSRVRIWLIIFFVPILLMLGGIGYLAWRQSVPAVRVEFSPVP
                     KFIGVRTPITLDLRARRGGVQSVDVRLLQGGARVNVAQQTFSGTPSNEQRVQLIVAGG
                     TLGLREGAATLEVRARDGFWRPIRVDDRTIASLPVVLDFTPPTLEILASTRYLSRGGG
                     GLVALRAKGAARVGVAVGDLFFPGFAAGAADSGLYATLYALPWNLPPNSPITVSAQDE
                     AGNAVSRALAVDIRPRKFPMDTIDISEQFLASKMPELLPERGQIPPDQLLAAFLIVNR
                     DKRKEAEEMKRKLAQKTKPTPLFEGAFIQPRNTKVFSNFAETRTYRYQGKDVDTQVHL
                     GYDLASLKHSPVPAANAGVVVYAAPLSIYGNAVVVDHGWGLQTLYGHLSTLEVKEGDE
                     VKKGQELGRSGATGLALGDHLHFEVLIQGISVTPVEWWDGKWIRDHVGRPLREANIPL
                     LQSDQPEAGAAEERPTAPSPSRRRARR"
     gene            complement(2142..>2504)
                     /locus_tag="VNU03_23905"
     CDS             complement(2142..>2504)
                     /locus_tag="VNU03_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77590.1"
                     /translation="VLLAGDAAGFYDPFTGEGLFTALRGAELLAEVAHPALTRDDVSA
                     AALAPYAAARRRAFADKARVTRALQLVIAHRRLANAAAHFLHRRPALLSMLMGVIGDF
                     IPPRAIWGRVFHSDISRA"
BASE COUNT          380 a          896 c          879 g          349 t
ORIGIN      
        1 gcgcggctcc acgaagcgga aatacatgaa cggaatcagg tagaacagca gcaggtgacc
       61 ggacagcagc cgcacgctcg ccgcgccttg gtcgcccagc aggaggcaga gcccgaggtt
      121 catcagcacg taggcgatca ccgccaccgc cgcgtgcttc ggcccccgcg ggatctgggc
      181 caggagcacg cgccactcca ccatccgcac cgtcccccgc ccgcgcgcgg gggcgcccga
      241 ctcgcgcagg cgcacgatca tggcggccag caggagaaac gcccccgcga gcaggacgag
      301 ggcgaccgcg gccgcgtagg cgggcgggaa cggggtgaag gtcaggaggt ggagcgcggc
      361 gctggagacg aagccgacgg tcgcggttac tcggaaaatc ttgccccagt ctagccgggt
      421 ggcggacacg ggtccctatc tagcatagac cggagcatct ctttctgatt cgctcgactc
      481 gccttcggct cgcctcgcaa ggcttcggcc gcgggcgacc cggtcccgcg gacgacccgg
      541 tctcgcaagc gacccggttc ggcggtcggc ccgtttttgc tctgctcgtt tcacgcgcaa
      601 aatttaggtg gggggcgctc cggctgctac catcgtcccg tggcacggtc gcgggttcgg
      661 atctggctga tcatcttctt cgtgcccatc ctcctgatgc tgggcgggat cggctatctc
      721 gcgtggcgac agagcgtgcc cgcggtgcgc gtggagttct ccccggtgcc gaagttcatc
      781 ggggtgcgca cgccgatcac gctcgatctg cgcgcgcgcc gcggcggggt gcagtcggtg
      841 gacgtgcggc tcctccaggg cggcgcccgc gtgaacgtgg cccagcagac cttctccggc
      901 acgccgagca acgagcagcg cgtgcagctc atcgtggccg gtggcaccct cgggctccgc
      961 gagggcgcgg ccaccctcga ggtgcgggcg cgcgacggct tctggcgacc gatccgggtg
     1021 gacgaccgca ccatcgcgag cctgcccgtc gtcctcgact tcacgccgcc caccctcgag
     1081 atcctcgcct ccacgcgcta cctctcgcgc ggcgggggtg ggctggtcgc gctgcgggcc
     1141 aagggcgccg cgcgggtcgg ggtggcggtg ggcgatctct tcttccccgg cttcgcggcc
     1201 ggcgcggccg actcgggcct ctacgccacg ctctacgcgc tgccctggaa cctgccgccc
     1261 aactcgccga tcacggtctc ggcccaggac gaggcgggca acgcggtctc tcgcgcgctc
     1321 gcggtcgaca tccgcccgcg caagttcccc atggacacca tcgacatcag cgagcagttc
     1381 ctcgcgagca agatgccgga gctcttgccc gagcgcggcc agatcccgcc cgaccagctc
     1441 ctcgccgcct tcctcatcgt gaatcgagac aagcggaagg aagcggagga gatgaagcgg
     1501 aagctcgccc agaagaccaa gcccacgccg ctgttcgagg gcgcgttcat ccagccgcgc
     1561 aacaccaagg tgttctcgaa cttcgcggag acgcgcacct accgctacca gggcaaggac
     1621 gtggataccc aggtgcatct gggctacgac ctggcgtcgc tcaagcacag cccggtgccg
     1681 gcggccaacg cgggcgtggt ggtctacgcg gcgccgctca gcatctacgg caacgcggtg
     1741 gtggtcgatc acggctgggg actgcagacg ctctacggcc acctctcgac cctggaggtg
     1801 aaggagggcg acgaggtgaa gaagggccag gagctcggcc ggagcggcgc caccggcttg
     1861 gcgctcggcg accacctgca cttcgaggtg ctcatccagg gcatctcggt caccccggtc
     1921 gagtggtggg acggcaagtg gatccgcgac cacgtcggcc gtccgctgcg cgaggccaac
     1981 atccccctgc tccagtccga ccagcccgag gccggcgccg ccgaagagcg cccgaccgct
     2041 ccctctccct cccgccgccg cgcccgccgc taggccgggg tcaggtctta cattccgaca
     2101 tttttctctg tgcccgtcgt ccgccccgcg atgacgcgcg gtcaggcccg cgaaatgtcg
     2161 gaatggaaga cccgacccca gatggcgcgc ggggggatga agtcgccgat cacgcccatc
     2221 agcatcgaga gcagcgcggg gcggcggtgc aggaagtggg cggccgcgtt ggcgaggcgg
     2281 cggtgggcga tgacgagctg gagcgcgcgc gtgacgcggg ccttgtcggc gaacgcccgg
     2341 cgccgcgcgg ccgcgtaggg cgccagcgcg gcggccgaga cgtcgtcgcg cgtgagagcc
     2401 gggtgcgcca cctccgccag cagctctgct ccgcgcagcg cggtgaagag accctcgccg
     2461 gtgaacggat cgtagaagcc cgccgcgtca cccgcgagca gcac
//
LOCUS       DATMCV010001042         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_657707, whole genome shotgun sequence.
ACCESSION   DATMCV010001042 DATMCV010000000
VERSION     DATMCV010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657707"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VNU03_23910"
     CDS             complement(<1..1197)
                     /locus_tag="VNU03_23910"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP77591.1"
                     /translation="MTARPGVRAEPTLLVSFDGGVRERTVCHRPDRYRLLERDVDSRA
                     RIARGGGYSYAAASFGGGAVTVEMQRFNRILGFDPARRTIEVEAGMTLGELLPVTARA
                     GLWLAVQPGYPAITVGGSIAANVHGKNPFRDGTFRSCVADLTLFHPDHGFRRLSETEG
                     ASAFELTCGGYGLTGIVVAATLRLEPLGGSRVSVKRHAIGSLSEAARVVQDLAGKSLF
                     AYTWHDATPRPRTFGRGYVYEGTLDPGGLDAGDVAAPYRVLTAAGRAGLPFSLWNAVS
                     ARVFASAYWHRARHRPEVTTGGLFDAIFPFARRSAYFRLFGRPGLVEYQAIVPFDRTE
                     SFLTELSRLILSAGPPSVLLSMKAFAGEPGRLRFEAGGLCLTLNLQRRATTLDLLARL
                     DELSIET"
     gene            complement(1194..>2537)
                     /locus_tag="VNU03_23915"
     CDS             complement(1194..>2537)
                     /locus_tag="VNU03_23915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77592.1"
                     /translation="WQLYQGSPRGLSTFLRSGWDVAGGYSSAMSSAFESWPIGVGAFL
                     LWIGLLVAWVVSWRASRPLRSLAVLAMPLFVAWKHSVVRQDVHVKILVLFGLFSMAIL
                     LLDAAVLGRWGRALPVVVLLTALLITSWYSLPAAFLELVRKAPVGPCPTNTLESNLSQ
                     PLRLCGLRGLAEWRDPAAMRRRLEAETRAELRPDLLPASMRTRIGSAAVDVYPWEIAY
                     VPANTLVWANRPLPASFNAYTASLDRLNAAFFESARRPPYLLWHAPSSGRGPLWSIDE
                     RYLLWDEPRTVRTILDRYDLVEASPGVLLLQSRGRPRFAGLAPIRHEVVDWNTWLDVP
                     ATSGVLLAHATIKPRTVARALRTVFRESAVFVSLRFSSGEEARFRIVPESAAGGFWVR
                     PFASTFEELPRLLAQGEGRTVVAIRFQLGRVARLYEPVAVTWSNLALADAPGPAR"
BASE COUNT          426 a          852 c          958 g          301 t
ORIGIN      
        1 cgtctcgatg ctcagctcgt cgagccgggc gagcaggtcc agggtcgtcg cgcgccgctg
       61 caggttcaga gtgagacaga gcccgcccgc ctcgaagcga aggcgccccg gctcgccggc
      121 gaacgccttc atggaaagca agaccgaggg cggaccggcc gagaggatca gacggctcag
      181 ctcggtcagg aaggactccg tccgatcgaa gggcacgatg gcctggtact cgacgaggcc
      241 cggccgtccg aacagacgga agtaggcgga tctccgggcg aacgggaaga tggcgtcgaa
      301 gaggccgccg gtggtgactt ccgggcggtg gcgcgcccgg tgccagtagg ccgacgcgaa
      361 gacgcgggcg ctgaccgcgt tccagaggga gaacggcagc ccggcccggc ccgcggcggt
      421 caggacgcga tacggggccg cgacatctcc cgcgtcgagg ccgcccggat cgagcgttcc
      481 ctcgtagacg tagcctcgcc cgaacgtccg cggtctcggg gttgcgtcgt gccacgtgta
      541 ggcgaagagg ctcttgccgg cgagatcctg caccacgcgc gctgcctcgg acagggagcc
      601 gatggcgtgg cgcttcacgc tgacccggct tccgccgagc ggctcgagtc ggagggtcgc
      661 ggcgaccacg atgccggtga gtccgtatcc cccgcaggtg agctcgaacg cggacgcgcc
      721 ctcggtctcc gacaggcgtc ggaagccgtg atcgggatgg aagagggtga ggtcggccac
      781 gcacgagcgg aaggtgccgt cccggaacgg gttcttgccg tggacattgg ccgcgataga
      841 gccgccgacg gtgatcgccg gatacccggg ctgcaccgcg agccagaggc cggcgcgcgc
      901 ggtcaccggg aggagctcgc cgagggtcat tcccgcttcg acctcgatgg tgcgccgagc
      961 cggatcgaag ccgaggatgc ggttgaaccg ctgcatctcg acggtcaccg ctcccccgcc
     1021 gaagctcgcc gcggcgtagg agtagccgcc gccccgggcg atcctcgcgc gcgaatcgac
     1081 atcgcgctcg agcaagcggt agcggtcggg ccgatgacac acggtgcgct cccggacgcc
     1141 gccgtcgaag gagacgagga gcgtgggctc ggcgcggact cccggacggg ccgtcatcgc
     1201 gccggtccgg gggcgtccgc gagggctaga ttggaccagg tcaccgcgac cggctcgtag
     1261 agccgggcga cccgaccgag ctggaagcgg atggccacga cggtgcggcc ttcgccctgg
     1321 gcgagcaggc gcggcagctc ctcgaaggtg gaggcgaacg ggcggaccca gaagccgccc
     1381 gccgcgctct cggggacgat gcggaatcgc gcctcctctc cggacgagaa ccggagggac
     1441 acgaacaccg cactctcccg gaaaacggtc cggagcgccc gcgccaccgt ccgcggcttg
     1501 atcgtcgcgt gcgcgaggag gacgccggac gtcgcgggca cgtcgagcca cgtgttccag
     1561 tccacgacct cgtgccggat cggggcgagt ccggcgaagc gaggccggcc gcgcgactgg
     1621 agcaggagaa cgcccgggga cgcctcgacc agatcgtagc ggtccaggat ggtgcgcacg
     1681 gtcctcggct cgtcccacag caggtatcgc tcgtcgatgc tccagagagg gccgcgcccg
     1741 ctcgagggag cgtgccagag gaggtacggc ggtctgcggg cggactcgaa gaacgcggcg
     1801 ttgagccggt cgaggctcgc ggtgtacgcg ttgaacgagg ccggcaacgg ccggttcgcc
     1861 cataccaggg tgttggccgg cacgtaggcg atctcccacg ggtacacgtc caccgcggcg
     1921 ctcccgatcc gggtgcgcat cgacgcgggc aggagatcgg ggcgcagctc ggctcgcgtc
     1981 tccgcctcga gtcggcgcct catggcggcg ggatccctcc actcggcgag gccgcgaagg
     2041 ccgcagagcc ggagcggctg ggagaggttg ctctcgaggg tgttggtcgg acagggcccg
     2101 acgggagcct tccgcaccag ctcgagaaat gcggcgggca gcgaatacca ggacgtgatc
     2161 aggagcgcgg tcagcaggac cacgaccggg agcgcgcgcc cccagcgccc gaggaccgcc
     2221 gcgtcgagga gcaggatcgc catcgagaag agcccgaaca ggaccaggat cttcacgtgg
     2281 acatcctgcc ggacgacgga gtgcttccac gcgacgaaga ggggcatcgc caggacggcg
     2341 agggaccgga gcggccgcga agcccgccag gacacgaccc acgcgacgag caggccgatc
     2401 cagaggagga acgcgcccac gccgatcggc cagctctcga atgcgctgct catcgccgag
     2461 gagtagcccc cggccacgtc ccatccgctg cggaggaagg ttgagagccc tcgcgggctg
     2521 ccctggtaga gctgcca
//
LOCUS       DATMCV010001043        10362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_323817, whole genome shotgun sequence.
ACCESSION   DATMCV010001043 DATMCV010000000
VERSION     DATMCV010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323817"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VNU03_23920"
     CDS             <1..526
                     /locus_tag="VNU03_23920"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWP77593.1"
                     /translation="MLLHFTRIAEATDRPILIYNIPYRTGVNLTNETLLRLAEIPNIA
                     GVKDSSGSIAQSLDLLRQRPDGFAVLTGEDAYFYTMLAHGGDGGILAAAHVETALFLT
                     VYERMAANDHQGARKAWSRIEPLVPLLFKEPNPVPIKHCLWRQGLIGSAECRLPLTRV
                     SDALAGELDRALGR"
     gene            complement(523..1323)
                     /locus_tag="VNU03_23925"
     CDS             complement(523..1323)
                     /locus_tag="VNU03_23925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77594.1"
                     /translation="MSPPAIPSPAATLVLMRDRPAGPAEILLIQRHGRSKFAAGDYVF
                     AGGKVEADDIPPDVEQFCRGLTPDQASARLGGDLTPRQALGYWVGAIREAFEEVGVLL
                     AYDRRGEFVRFTAANRARFEAHRKACQSANPAFFTMLREEGLTLATERLTYFAHWITP
                     EEQPLRFDTRFFAAEAPPGQEPEVDGHEIVALRWLTPAEAFAALRGKEITLRLPTIKN
                     LELLQGAGARTAEILGALRGRPVPTIRPRVLQVDGKPVPVLPGDPRWY"
     gene            1430..1750
                     /locus_tag="VNU03_23930"
     CDS             1430..1750
                     /locus_tag="VNU03_23930"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWP77595.1"
                     /translation="MQSPHTAISLSPAEHRRHARVAASWPVTVQTGDRLLHLHAVNVS
                     ALGVKVSLDEPLRREPLEVGRPAHLRMEPPGAQPVEVEAIVWRTDDDGPAFFFIGPRA
                     AASR"
     gene            1783..2319
                     /locus_tag="VNU03_23935"
     CDS             1783..2319
                     /locus_tag="VNU03_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP77596.1"
                     /translation="MGAEARIKELGITLPNPPKPMGNYVPGVRTGNLLFLSGHGPIRI
                     EGNPSARGKVGRDLSTEDAYKVAREVGINLLGSARALLGSLDRVKRVVKVLGMVNAVE
                     SFGEQPKVINGFSDLMVEVFGDAGRHARSAVGMGSLPAGIPVEIEMILEVGAPAAPKA
                     RKPARAAKAPTRKPKRRR"
     gene            complement(2316..3080)
                     /locus_tag="VNU03_23940"
     CDS             complement(2316..3080)
                     /locus_tag="VNU03_23940"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HWP77597.1"
                     /translation="MPARRVVSLIPSITEILFAIGAGDRVVGCTIYCTEPPEGVATKT
                     RVGGEKNPKLEVIRDLGTDLVIANVEENVREHVETLRGWGIAVHVTYPRTVADGIRLV
                     GELGVLLEVGERARELEAELQARYDRVRAAAAGRRPRRVFCPIWRKPYMTINRDTYIH
                     DMLAVAGGANVFRDEATRYPQITLEQVAASEAEVILLPDEPYRFRRVHEADFAPFPDL
                     PAVRDGRIHLVDGKLLSWYGPRIAQALEILPPLLEA"
     gene            complement(3089..3271)
                     /locus_tag="VNU03_23945"
     CDS             complement(3089..3271)
                     /locus_tag="VNU03_23945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77598.1"
                     /translation="MSQDTTRRLLREFGVAVTSFEDAVEGGQGEAARKAEALLREHMK
                     ELIGLVERLSEQAAKL"
     gene            3286..4296
                     /locus_tag="VNU03_23950"
     CDS             3286..4296
                     /locus_tag="VNU03_23950"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77599.1"
                     /translation="MRPVHDMVGALDLLYRAFYRGVLARLPERGAVAIGQWGLRHLPL
                     DRLGMFRNPDPRLAMTLGGVRLANPLILSSMYYDTAILRRAMGLGWGAVTAKSITPGP
                     RPGHPEPNLVRIQTAEGPGLVNCNGFKNPGLEAYRSALAALPHRVPLIVAAAGESAED
                     YVRVVAGLQEFGDLVELNISSPNTKLVYGWSTRPAELKDVFRAVRAATPKPLVVKISP
                     DFRETNEEITIPAALDAGITIVNYGNTRRVDEPRLSQRTGGLSGPALFATTLENVRRV
                     RERFGDRLELIATGGIDSPDSVRRALDAGATACGIFTGFITRGPLLPRRILDALAAAR
                     QK"
     gene            4572..4814
                     /locus_tag="VNU03_23955"
     CDS             4572..4814
                     /locus_tag="VNU03_23955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77600.1"
                     /translation="MEASRKPLAKIEGRKRMRHNGLTVAWRGTPSLDDWVAYIVTGTK
                     SKKLILADHASERKVKGLLARIQSLSKKEVEKLAKG"
     gene            complement(4811..5557)
                     /gene="radC"
                     /locus_tag="VNU03_23960"
     CDS             complement(4811..5557)
                     /gene="radC"
                     /locus_tag="VNU03_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWP77601.1"
                     /translation="MNPRPGACYGMRMGAAGSGIGRWPAEDRPRERLYHKGPESLADA
                     ELLAIQLGAGLAGCSAMDMARDLLSRYGSLSGLAGRGIAELAAVRGVGPVKAVRLAAA
                     FEITRRLRSRNGPGRVVLGSPEQVFARYGPLMEDLQKEVFRVALLDAQNGLLRDVVIS
                     EGTLSASLVHPREVFKPAILESAASIILLHNHPSGDPTPSREDLRLTRQLVDCSELLD
                     LRIHDHVIIGRQRFISLAQRGELGREGRAP"
     gene            complement(5554..5994)
                     /gene="tsaE"
                     /locus_tag="VNU03_23965"
     CDS             complement(5554..5994)
                     /gene="tsaE"
                     /locus_tag="VNU03_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWP77602.1"
                     /translation="MSRRTFASSDPAETQAIGERLGARLTAGAVIACTGELGAGKTCF
                     LQGLARGLGVTSGVTSPTFVLVNQYRGRMPIYHLDAYRTGSLTELVDLGLEEMLHGDG
                     VTVIEWADKLLPLLPARTIHVHLQGLGDEPREITIDEPVSDPAH"
     gene            complement(5991..7550)
                     /locus_tag="VNU03_23970"
     CDS             complement(5991..7550)
                     /locus_tag="VNU03_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWP77603.1"
                     /translation="MRPIFTAAEMRALDARAIRELGIPGFHLMDVAGSGAAALIGRWL
                     APIRGRRIVVVCGKGNNGGDGFVVARRLRARGAAVTVFLTARRDEVQGDAAEALAGWR
                     GRVEAIDAGPDPGALVRALERAEAVVDALLGTGLTGPARGQAAAAIEAINEAGRRGIP
                     VIALDLPSGLSSDHGALLGPTVKATRTVTFAGLKRSLLLHPAAGQTGPVDVVDIGVPE
                     AEAARGITTWRLEAADVRRYFPAREADAHKGRFGHLLVVAGSLGKTGAAVLAARAALR
                     SGVGLCTIAAPASQQPIVAAQAPEYMTEALPETAAGSLALAGRQRLLELAHRMDAVAL
                     GPGLSLHPETQELARALVAEVERPLVADADALSALAGHLDLLRRALGPRALTPHPGEM
                     ARMLGLTVDAVQGDRLEIARSFGRDHHVGLALKGAGTVIGGPDGRVAVNPTGNPGMAK
                     GGSGDVLTGIVGALLARGLDPVAALEAGCYVHGLAGDLAGAERGEIAMIAGDIVEKLP
                     AALQALAGQPS"
     gene            complement(7555..8322)
                     /locus_tag="VNU03_23975"
     CDS             complement(7555..8322)
                     /locus_tag="VNU03_23975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77604.1"
                     /translation="MALGLALVVQGCAGLIAGKDSADAPAVAPTGPAGPITGSVQVEK
                     GRPGIVLAAPHGTSDSATDLMGRELAKLTGFSLVVVTGYTHVDASGRRYNVNRPTESQ
                     PGAAARLEVETEPARQLYQAYRRQVTEVSQGPLRLYVEVHGNMRPESAGRMEIATVGV
                     SRDDAWRLKALLEMIRDARLPAEGEVPRLDVWMEPLDTLWYTASAAKQSGMLAQTRLA
                     LHIELPRAARTTYREAYTTVLADFLIQSAALLLPPAR"
     gene            complement(8324..8737)
                     /gene="acpS"
                     /locus_tag="VNU03_23980"
     CDS             complement(8324..8737)
                     /gene="acpS"
                     /locus_tag="VNU03_23980"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWP77605.1"
                     /translation="MSRVLGIGVDLTQMPRMRRVVARWDERFLQRVFTEQEIAYCRRR
                     RDPIPHFAARFAAKEATLKALGTGLRMGVNWRELEVRRERGQAPTMTLSGRSRAIALA
                     KGGVQVLLSLTHDGEYAMAQAMLIGDGADDEARRR"
     gene            complement(8734..10083)
                     /gene="glmM"
                     /locus_tag="VNU03_23985"
     CDS             complement(8734..10083)
                     /gene="glmM"
                     /locus_tag="VNU03_23985"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWP77606.1"
                     /translation="MTRLFGTDGIRGLANKPPLTTELAFRLGRQLVATLLEHDRGHKT
                     RLVVGRDTRLSGPMLEGALVSGALSAGGDVYAVGVLPTPAIAYLTRRLEAHGGVVLSA
                     SHNPFEDNGIKIFSSEGAKFPDAWEEEIEGRLSGPDTAPWPTGMDIGRLVQHEAAEAD
                     YITHARATCPSDLRGLRVVLDCAHGATYRVAPEVFRRLGADVRVLNASPDGENINRGV
                     GAMHPEMLQKEVLRTRAQVGLAFDGDGDRLISVDEDGHIRDGDFALAICGRHLASRGL
                     LKSGTVVTTVMANLALDRVLTRAGIGIAKVQVGDRYVLEEMLRVGANLGGEQSGHLLF
                     LDHSTTGDGIVSALQLIGVMQETRSSLGELSKCLVKFPQVLLNVTVKEKPPVESIPGL
                     AERARALEADMNGAGRILLRYSGTESLLRVMIEGEEQARIERMAEELAGLVRRSIGA"
     gene            complement(10080..>10362)
                     /locus_tag="VNU03_23990"
     CDS             complement(10080..>10362)
                     /locus_tag="VNU03_23990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77607.1"
                     /translation="VVVAAPIELDGIPAGLEVKGERPDSVDVQLHGLRVALDRLGTDR
                     VRARVSLAGSRAGEVTLRVQPEQITVPPGVTVIRVNPSRLRLVLGAVRS"
BASE COUNT         1446 a         3791 c         3733 g         1392 t
ORIGIN      
        1 catgctccta cacttcacgc gcatcgcgga ggccaccgac cggccgatcc tgatctacaa
       61 catcccctac cgcaccgggg tcaacctgac caacgagacc ttgctgcgtc tggccgagat
      121 cccgaacatc gcgggggtga aggactcctc cggcagcatc gcgcagtcgc tcgatctgct
      181 gcgccagcgg cccgacggct tcgcggtgct caccggcgag gacgcctact tctacacgat
      241 gctcgcccac ggcggcgacg gcggcatcct cgccgcggcg cacgtcgaga ccgcgctgtt
      301 cctcaccgtg tacgagcgca tggcggccaa cgaccaccag ggcgcgcgca aggcctggtc
      361 gcggatcgag ccgctcgtgc cgctgctctt caaggagccg aacccggtcc cgatcaagca
      421 ctgcctctgg cgccaggggc tcatcggctc cgccgagtgc cgcctgccgc tcacgcgcgt
      481 gtccgacgcg ctcgcgggcg agctcgaccg cgccctcggc cgctagtacc agcgaggatc
      541 gccgggcagg accgggacgg gcttgccgtc cacctgcagg acccggggac ggatggtagg
      601 caccgggcgg ccgcggagcg cgcccaggat ctcggcggtg cgggctccgg cgccctgcag
      661 cagctccagg ttcttgatgg tgggcagccg gagggtgatc tccttccccc ggagcgccgc
      721 gaaggcctcc gccggcgtca gccagcgcag ggccacgatc tcgtgcccat cgacttccgg
      781 ctcctgcccg ggcggagcct ccgcggcgaa gaaccgggtg tcgaagcgca gcggctgctc
      841 ctcgggcgtg atccagtgcg cgaagtaggt gaggcgctcg gtggccagcg tcaggccttc
      901 ctcgcgcagc atcgtgaaga aggcggggtt cgccgactgg cacgccttgc gatgggcctc
      961 gaagcgggcg cggttggccg cggtgaaccg cacgaactcg ccccggcggt cgtacgccag
     1021 cagcactccc acctcctcga aggcctcgcg gatggctccc acccagtagc cgagggcctg
     1081 gcggggggtc aggtcgccgc ccagccgggc actcgcctga tccggggtga gcccgcggca
     1141 gaactgctcc acgtcggggg gtatatcgtc ggcctcgacc ttgccgccgg cgaagacgta
     1201 gtccccggcc gcgaacttgc tgcgcccgtg gcgctggatg agcaggatct cggccgggcc
     1261 ggcgggccgg tcgcgcatga ggaccagggt cgcggccggt gaaggaattg caggtgggct
     1321 catgggaatc ggtcctcgaa accgttcgca ttctgacacg gtccgccggc gcggggtcgg
     1381 gcaaaaaaag gcacttgtga aggcggtggg ccgatgggaa actcgccccg tgcagagccc
     1441 gcacaccgcc atcagcctct cgccggcgga gcatcgccgg cacgcacggg tggcggcctc
     1501 gtggccggtg acggtccaga ccggcgatcg cctcctccat ctccacgcgg tgaacgtgag
     1561 cgcgctcggc gtcaaggtct cgctcgacga gccgctgcgg cgcgagccgc tcgaggtcgg
     1621 ccgccccgcg catctgcgca tggagccccc gggcgcgcag ccggtggagg tcgaggcgat
     1681 cgtctggcgc accgacgacg acgggcccgc gttcttcttc atcggcccgc gcgcggccgc
     1741 gtcgcggtag tcgactcacc cctttcccac gaggagaccg cgatgggcgc agaagccagg
     1801 atcaaggagc tcggcatcac gctgcccaac ccgcccaagc cgatgggcaa ctacgtcccg
     1861 ggcgtccgga ccggcaacct gctcttcctg tcgggccacg gcccgatccg catcgagggc
     1921 aacccgagcg cccgcggcaa ggtcgggcgg gacctctcca ccgaggacgc ctacaaggtg
     1981 gcgcgcgagg tcggcatcaa cctgctgggc tccgcgcgcg cgctcctggg cagcctcgac
     2041 cgcgtcaagc gggtggtgaa ggtgctcggc atggtgaacg cggtcgagag cttcggcgag
     2101 cagcccaagg tcatcaacgg cttctcggac ctcatggtcg aggtgttcgg cgacgccggg
     2161 cgccacgccc gctccgcggt gggcatgggc tcgctcccgg ccggcattcc ggtggagatc
     2221 gagatgatcc tcgaggtggg cgcgcccgcg gcgccgaagg cccgcaagcc ggcgcgggcc
     2281 gcgaaggcgc cgacccgcaa gccgaagcgc cgccgctagg cctcgaggag cggcgggagg
     2341 atctcgaggg cctgcgcgat ccgcgggccg taccaggaca gcagcttgcc gtccacgagg
     2401 tggatccgcc cgtcgcgcac cgcgggcagg tcggggaacg gcgcgaagtc cgcctcgtgg
     2461 acccggcgga accggtaggg ctcgtccggg agcaggatca cctccgcctc cgaggccgcc
     2521 acctgctcga gcgtgatctg ggggtagcgg gtcgcctcgt cccggaagac gttggcgccg
     2581 ccggccaccg ccagcatgtc gtgaatgtag gtgtcccggt tgatcgtcat gtagggcttg
     2641 cgccagatgg ggcagaacac gcggcgcggc cgccgccccg cggcggcggc ccgcacccgg
     2701 tcgtagcgcg cctggagctc cgcctcgagc tcgcgcgccc gctcccccac ctcgagcagc
     2761 acccccagct cccccaccag ccggatgccg tcggcgacgg tgcgggggta cgtgacgtgg
     2821 accgcgatgc cccacccccg gagcgtctcg acgtgctcgc gcacgttctc ctcgacgttg
     2881 gcgatgacga ggtcggtgcc caggtcgcgg atgacctcga gcttggggtt cttctcgccg
     2941 ccgacccggg tcttggtggc cacgccctcg ggcggctccg tgcagtagat cgtgcagccc
     3001 accacgcggt cccccgcgcc gatggcgaac aggatctcgg tgatgctggg gatcagggag
     3061 acgacgcgcc gggccggcac cggcggagct agagcttggc ggcctgctcg gaaaggcgct
     3121 cgacgagccc gatcagctcc ttcatgtgct cgcggagcag cgcctcggcc ttccgcgcgg
     3181 cctcgccctg cccgccctcg accgcatcct cgaagctggt gacggcgacg ccgaactccc
     3241 tcagcaagcg acgcgtggtg tcttgcgaca tggttgtagg atatcatgcg cccggtccac
     3301 gacatggtcg gcgccctcga tctcctctac cgcgcgttct accgcggcgt gctggcgcgg
     3361 ctgcccgagc gcggcgcggt ggcgatcggc cagtggggcc tgcgtcacct gccgctcgat
     3421 cgcctcggga tgttccgcaa tcccgatccg cggctcgcca tgacactggg cggggtgcgc
     3481 ctggcgaatc cgctgatcct ctcgtccatg tactacgaca ccgcgatcct ccgtcgggcc
     3541 atgggcctcg ggtggggcgc ggtcaccgcc aagagcatca cccccggccc gcggcccggg
     3601 catcccgagc ccaacctggt gcgcatccag accgcggagg ggccggggct ggtcaactgc
     3661 aacgggttca agaatccggg cctcgaggcc taccgctccg cgctggccgc gctgccgcac
     3721 cgcgtgccgc tgatcgtggc cgcggcgggg gaatccgcgg aggactacgt gcgggtggtg
     3781 gcgggcctgc aggagttcgg cgacctggtc gagctcaaca tctcctcgcc caacaccaag
     3841 ctggtctacg gctggtccac gcggcccgcc gagctgaagg acgtgttccg ggccgtccgg
     3901 gccgccaccc ccaagccgct cgtcgtcaag atctcgcccg acttccggga gaccaacgag
     3961 gagatcacga tcccggccgc gctcgacgcg ggcatcacga tcgtcaacta cggcaacacc
     4021 cggcgggtgg acgagccgcg gctctcccag cggaccggcg gcctctcggg gcccgccctc
     4081 ttcgcgacca cgctcgagaa cgtccgccgg gtccgtgagc gcttcggcga ccgcctcgag
     4141 ctgatcgcga ccggcggcat cgacagcccc gattccgtgc gtcgggcgct cgacgcgggg
     4201 gccaccgcgt gcgggatctt caccggattc atcacccggg gcccgctctt gccgcgacgc
     4261 atcctcgacg cgctcgcggc tgcccgccag aaataaccca caggttatcc acttatccac
     4321 cgtcatccac aatcgcgaaa aaggcacgct gcgacgcggt ccgagagggg ggtccccgaa
     4381 aagtgacgct ctgatcgtcc accgccgcga ggcctcacat cacaggcagc attcacgcag
     4441 gctcacgcgc gccgaggcgc caaattacgc ttgtgttgcg gccaaacgaa gccttgacag
     4501 ggtcgggacc ggtccataag gttgcgtggc gcagcaccgt gtgcaccctt cggaggagga
     4561 gaagatcgcg tatggaggcc agccggaagc cactggcgaa gatcgagggc aggaaacgga
     4621 tgcggcacaa cggtctcacg gtggcgtggc ggggcacgcc gagtctcgac gactgggtgg
     4681 cctacatcgt caccggcacg aagtccaaga agctgatcct ggccgatcac gcctccgagc
     4741 ggaaggtgaa gggcctgctg gcccgcatcc agtccctgtc caagaaggag gtcgagaagc
     4801 tcgccaaggg ctagggcgcg cggccctcac ggcccagctc gccgcgctgg gccaggctga
     4861 tgaatcgctg gcggccgatg atgacgtggt cgtggatgcg caggtcgagc agctccgagc
     4921 agtccacgag ctggcgggtg agccgcaggt cctcccggct cggggtcgga tcgccgctcg
     4981 ggtggttgtg cagcaggatg atcgaggcgg cggattccag gatcgccggc ttgaagacct
     5041 cgcgggggtg cacgagactg gccgagagcg tgccttccga gatcaccacg tcccggagga
     5101 gcccgttctg ggcgtccagc agcgccaccc ggaacacctc cttctggagg tcctccatca
     5161 gcggcccgta gcgcgcgaac acctgctcgg ggctccccag gaccacccgg cccggcccgt
     5221 tccgcgagcg caggcgccgg gtgatctcga acgcggcggc caggcgcacc gccttcaccg
     5281 gccccacccc gcgcaccgcg gccagctcgg cgatcccgcg cccggccagc cccgagagcg
     5341 acccgtagcg gctcagcaga tcgcgcgcca tgtccatcgc gctgcagccg gcgagaccgg
     5401 cgccgagctg gatggcgagc agctcggcgt cggcgaggga ctcggggccc ttgtggtaga
     5461 gccgctcgcg gggccggtcc tccgccggcc accgcccgat gccgctcccc gccgcgccca
     5521 tgcgcatgcc gtagcacgcg cccgggcgcg ggttcaatga gccggatcgg atacgggctc
     5581 gtcgatggtg atctcgcgcg gctcgtcgcc gagcccctgg aggtgcacgt ggatcgtccg
     5641 cgcgggcagc agcggcagga gcttgtccgc ccactcgatc acggtgacgc cgtcgccgtg
     5701 cagcatctcc tcgaggccca ggtccaccag ctcggtgagg ctgccggtcc ggtaggcgtc
     5761 gagatggtag atcggcatcc ggccgcggta ctggttgacc agcacgaagg tcgggctcgt
     5821 cacgccggag gtcaccccga gcccgcgcgc gagcccctgc aggaagcagg tcttccccgc
     5881 ccccagctcg ccggtgcacg cgatgaccgc gcccgcggtc aggcgtgcgc ccaggcgctc
     5941 gccgatcgcc tgcgtctcgg cgggatcgct cgacgcgaac gtccgccggc tcacgacggc
     6001 tggcccgcga gggcctggag cgccgcgggc agcttctcga cgatgtcgcc cgcgatcatc
     6061 gcgatctcgc cccgctcggc cccggccagg tctccggcca ggccgtggac gtaacagccc
     6121 gcctcgagcg cggcgaccgg atcgagccct cgcgcgagca aagcgccgac gatcccggtg
     6181 agcacgtcgc ccgacccgcc cttggccatg ccgggattgc cggtgggatt gaccgccacg
     6241 cgtccgtcgg gcccgccgat cacggtgccc gcgcccttga gcgcgagacc cacgtggtgg
     6301 tcgcggccga agctccgggc gatctccaat cggtcgccct gcaccgcgtc gaccgtcaga
     6361 ccgagcatgc gcgccatctc gccggggtgc ggggtcaggg cgcgcgggcc gagcgcgcga
     6421 cgcagcagat ccaggtgccc ggccagcgcg ctgagcgcgt cggcgtccgc caccaggggc
     6481 cgctccacct cggccaccag ggcgcgggcc agttcctgcg tctcgggatg gagcgagagc
     6541 ccgggcccga gcgcgactgc gtccatgcgg tgggccagct cgaggaggcg ctgccgcccg
     6601 gccagcgcga ggctgcccgc ggcggtctcg ggcagcgcct cggtcatgta ctccggcgcc
     6661 tgcgcggcga cgatgggctg ctgcgatgca ggagccgcga tcgtgcagag accgaccccg
     6721 ctccggagcg cggcgcgggc ggcgagcacc gcggcgccgg tcttgcccag cgagcccgcc
     6781 acgaccagga gatggccgaa gcggcccttg tgggcgtcgg cctcgcgcgc ggggaaatac
     6841 cggcgcacgt cggcggcctc gagccgccag gtggtgatgc cgcgcgcggc ttccgcctcc
     6901 ggcaccccga tgtcgacgac gtcgaccggc ccggtctgcc cggcggcggg gtggagcagc
     6961 aggctccgct tgagcccggc gaaggtgacg gtccgcgtgg ccttgacggt cggcccgagc
     7021 agcgcgccgt gatcggagga gagccccgac ggcagatcga gcgcgatgac cgggatcccg
     7081 cgccgtcccg cctcgttgat cgcctcgatg gcggcggccg cctggccccg ggccgggccg
     7141 gtgagcccgg tgccgagcag cgcgtccacc accgcctcgg cccgctcgag ggcccgcacg
     7201 agcgcgcccg ggtccggccc cgcgtcgatg gcctcgaccc gccctcgcca gcccgcgaga
     7261 gcctccgcgg cgtcgccctg cacctcgtcg cgccgcgcgg tcaggaacac ggtcaccgcg
     7321 gcgccgcggg cccggagacg ccgcgccacc acgaagccgt cgccgccgtt gttgcccttg
     7381 ccgcacacga cgacgatgcg ccggccgcgg atgggggcga gccatcggcc gatgagcgcg
     7441 gccgcgcccg agccggccac gtccatgagg tggaagccgg ggatgccgag ctcgcggatc
     7501 gcgcgagcgt cgagcgcgcg catctcggcg gcggtgaaga tgggccgcac gggatcagcg
     7561 cgcgggcggc agcagcaggg ccgccgactg gatgaggaag tcggcgagca ccgtcgtgta
     7621 ggcctcccgg taggtggtcc gggccgcgcg cggcaactcg atgtggagcg ccaggcgggt
     7681 ctgggcgagc atgcccgact gcttggccgc ggaggcggtg taccagagcg tatcgagagg
     7741 ctccatccag acgtcgagcc ggggcacctc gccctccgcg ggcaggcgtg cgtcgcggat
     7801 catctcgaga agcgccttga gccgccacgc gtcgtcgcgg ctgacgccga cggtggcgat
     7861 ctccatacgg cccgcgctct ccggacgcat gttgccgtgc acctccacgt agagacgcag
     7921 cgggccctgc gacacctcgg tcacctggcg ccggtacgcc tggtagagct ggcgggccgg
     7981 ctcggtctcc acctcgagcc gcgccgccgc gccgggctgg ctctcggtgg gccggttgac
     8041 gttgtagcgc cgaccgctcg cgtcgacatg ggtatagccg gtcaccacca ccaggctgaa
     8101 tccggtgagc ttcgcgagct cgcgccccat gaggtcggta gcgctgtcgg aggtgccgtg
     8161 cggggccgcg aggacgatcc cgggccggcc cttctccacc tggacggatc cggtgatcgg
     8221 cccggccgga cccgtgggag cgaccgccgg cgcgtccgcc gaatccttgc ccgcgatcag
     8281 cccggcgcag ccctggacga cgagggcgag cccgagcgcc acgctagcgg cgcctcgcct
     8341 cgtcatcggc gccgtctccg atcagcatgg cctgcgccat cgcgtactcg ccgtcgtggg
     8401 tgagggagag cagcacctgc acgccgccct tggcgagcgc gatggcgcgc gagcgcccgg
     8461 agagcgtcat ggtgggagcc tgaccgcgct cgcgccgcac ctcgagctcg cgccagttga
     8521 cccccatgcg caggccggtg ccgagggcct tgagggtggc ctccttcgcc gcgaagcgcg
     8581 ccgcgaagtg cgggatcgga tcgcgccggc gccggcagta ggcgatctcc tgctcggtga
     8641 agacccgctg gagaaagcgc tcgtcccagc gcgccaccac ccgtcgcatc cgcggcatct
     8701 gggtgaggtc gacgccgatg ccgagcaccc ggctcacgcc ccgatgctcc gccgcacgag
     8761 gccggccaac tcctcggcca tgcgctcgat acgcgcctgc tcctcgccct cgatcatcac
     8821 gcgcaggagc gactcggtgc cggagtagcg cagcaggatg cgccccgcgc cgttcatgtc
     8881 ggcctcgagg gcgcgggcgc gctcggcgag cccggggatg ctctccaccg gcggcttctc
     8941 cttcaccgtg acgttcagca gcacctgggg aaacttcacc aggcacttcg acagctcgcc
     9001 cagtgaggac cgcgtctcct gcatcactcc gatgagctgc agcgcggaca cgatcccgtc
     9061 cccggtggtg ctgtgatcga ggaacagcag gtggccggac tgctcgccgc ccaggttggc
     9121 gccgacgcgc agcatctcct ccagcacgta gcggtcgccc acctgcacct tggcgatccc
     9181 gatgcccgcg cgggtgagca cgcggtcgag cgcgaggttg gccatcacgg tggtgaccac
     9241 cgtgccgctc ttgaggagcc cgcgggacgc gaggtggcgg ccgcagatgg cgagcgcgaa
     9301 gtcgccgtcc cggatgtggc cgtcctcgtc gaccgagatc aggcggtcgc cgtcgccgtc
     9361 gaaggcgagg cccacctggg cccgcgtgcg gagcacctcc ttctgcagca tctccgggtg
     9421 catcgccccc acgccccggt tgatgttctc gccgtccggg ctcgcgttca gcacccgcac
     9481 gtcggctccc aggcggcgga agacctcggg ggccacccgg taggtcgcgc cgtgggcgca
     9541 gtcgagcacc acccggaggc cgcgcaggtc ggacgggcac gtggcccgcg cgtgggtgat
     9601 gtagtcggcc tccgcggcct cgtgctggac gaggcggccg atgtccatgc cggtgggcca
     9661 gggcgcggtg tcgggaccgc tcaggcgtcc ctcgatctcc tcttcccacg cgtcggggaa
     9721 cttggccccc tccgaggaga agatcttgat gccgttgtcc tcgaaggggt tgtgggaggc
     9781 cgagaggacg acgccgccgt gggcctcgag ccgccgggtc aggtaggcga tggcgggagt
     9841 gggcagcacc ccgaccgcgt agacgtcccc gcccgcggag agggcgccgg agacgagcgc
     9901 gccttcgagc atcggcccgg agagccgcgt gtcgcggccg acgaccagcc gggtcttgtg
     9961 gccccggtcg tgctcgagca gggtggccac gagctggcgc ccgaggcgga aggccagctc
    10021 ggtggtgagc ggcggcttgt tggcgaggcc tcggatgccg tcggtgccga agagacgggt
    10081 cacgagcgca cggcccccag gacgaggcgg aggcgggagg ggttgacccg gatgacggtg
    10141 accccgggcg gcaccgtgat ctgctcgggc tgcacgcgca gggtcacctc gcccgcgcgc
    10201 gagccggcca ggctcacgcg ggctcgcacg cggtcggtcc cgaggcggtc gagcgcgacc
    10261 cgcaggccgt gcagctgcac gtccacgctg tcgggccgct cccccttgac ctcgagcccg
    10321 gccggaatgc cgtcgagctc gatcggcgcc gcgaccacca cg
//
LOCUS       DATMCV010001044         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_855914, whole genome shotgun sequence.
ACCESSION   DATMCV010001044 DATMCV010000000
VERSION     DATMCV010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855914"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VNU03_23995"
     CDS             <1..567
                     /locus_tag="VNU03_23995"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77608.1"
                     /translation="GLPYYAHDAALSRRLLAEAGFPNGLDTTLEVSPRFPQTVRTGEV
                     MKEQLASAGIRVTLKQTEWGAALKNFVRTEYDGMSMIPLVWQPDPDAHVYDIFHSGSA
                     INLGKFKDPRVDQLLEQGRTTLDRDKRIAIYRDLQRHMADQAYLVFPYASGATEALRD
                     TVKDYVSIPGAQPGSRSRHFFKQAWIDR"
     gene            552..1514
                     /locus_tag="VNU03_24000"
     CDS             552..1514
                     /locus_tag="VNU03_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77609.1"
                     /translation="MDRPLTVYVGKRLLLLIPTLLGVSVLTFLLIHLIPGDLVTILLG
                     ITASQNEQTRQALIHALHLDRPLAVQYLLWLGEVLRGDLGHSLVTGFSVRDELARSYP
                     VTLQLALMAMSIALAVGVPLGVLAATRAGRALDASTRVFSLLFISAPAFFIGTVVIIG
                     GARYAPWLPTLGHVPFTESPARSLGAMLPAAASLGLAISAIIARFTRSSMLEVLGQDF
                     VRTARAKGVADRAVVYKHALRNALIPVVAMAGTQFVYLIGGAVIIEEVFALPGMGRLV
                     VNAIYQRDYTMIQGAILLLTSAAVLVNLALDLLYHLIDRRILYA"
     gene            1529..2383
                     /locus_tag="VNU03_24005"
     CDS             1529..2383
                     /locus_tag="VNU03_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77610.1"
                     /translation="MTGPRVGRRAMRSGSLLAGGLIVAGLLVAALAAPWLAPYAPTTI
                     FPGAEIQPPGPRFLLGTDEVGRDILSRVLYGSRVSLGVAIPSVALAVLTGTTLGVGLG
                     YWGGVTDLLAMRIFDVLFAFPPILLAIALVAALGPSTVNLVLTIAVLTLPQFAVLARS
                     ATLALKPQEFVQAARALGASHGRILGAHVLPNLTPTLAVQTSLSLSIVILVEAALSFL
                     GLGTQPPAPSWGGMLSRGRQYMTIAPWLVLAPGLAIMLAVLGFNLLGDAQRDLLDPRR
                     AGTGRRTP"
     gene            2380..3216
                     /locus_tag="VNU03_24010"
     CDS             2380..3216
                     /locus_tag="VNU03_24010"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWP77611.1"
                     /translation="MSPSVRIAAVQARPQSDLFDDMWNGGDVAHAVDLLEAAARAGAA
                     CVCFPELYPRVGEAEIRAAARRLGVFVIAGLIEGTRASWHNTSTVIGPDGRILARQPK
                     CFPTQNEIDNGVAAGKGYRVVETDIGRLGIVICADFAFFSGGVESLVEQGVDIIFNPS
                     WWFALGEAYPATVIGRHMQYGKPVIGVDIAACALRLRGADGRPVERFPRAGGYSTVCV
                     APPIASLPELAAWFRTKAGGTNSAQGFIQSLGEEEGILYADVDIAAVRRFPGYFYRSA
                     NP"
     gene            3213..>3466
                     /locus_tag="VNU03_24015"
     CDS             3213..>3466
                     /locus_tag="VNU03_24015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77612.1"
                     /translation="MSPQDLLDELVQAAQRLGVEVRSEPFETPAALGGGLCVVRGAHL
                     VLIDQRAPLVDRLRALARALGDLGSDAVYMAPEARELVEGS"
BASE COUNT          440 a         1320 c         1169 g          537 t
ORIGIN      
        1 ggcctcccct actacgccca cgacgcggcc ctgtcgcggc gcctgctcgc cgaggcgggc
       61 ttccccaacg gcctcgacac gaccctcgag gtgtcgcccc gcttcccgca gacggtgcgg
      121 accggtgagg tcatgaagga gcagctcgcc tcggccggca tccgcgtgac cctcaagcag
      181 accgagtggg gcgcggcgct gaagaacttc gtgcgcaccg agtacgacgg catgagcatg
      241 atcccgctgg tgtggcagcc cgatcccgac gcccacgtct acgacatctt ccacagcggc
      301 tcggccatca acctgggcaa gttcaaggac ccgagggtcg accagctcct cgagcagggc
      361 cgcaccacgc tcgatcggga caagcggatc gcgatctacc gggacctcca gcgccacatg
      421 gccgaccagg cctacctggt cttcccgtac gcctcgggcg cgacggaagc gctgcgcgac
      481 accgtgaagg actacgtctc catccccggg gcccagcccg gaagtcggag ccggcacttc
      541 ttcaagcagg cgtggatcga ccgctgaccg tctacgtcgg caagcggctc ctgctcctga
      601 tcccgacgct gctcggggtg tcggtgctca ccttcctgct catccacctg atcccgggcg
      661 acctcgtcac catcctcctg ggaatcaccg cgagccagaa cgagcagacc cgacaggccc
      721 tgatccacgc actccacctg gaccgcccgc tcgcggtgca gtacctgctg tggctgggcg
      781 aggtgctgcg gggcgacctc ggccactccc tggtcaccgg gttctcggtg cgcgacgagc
      841 tggcccggag ctacccggtc acgctccagc tcgcgctgat ggccatgtcc atcgcgctgg
      901 cggtgggcgt cccgctgggc gtcctcgccg cgacccgggc cgggcgcgcc ctcgacgcct
      961 ccacgcgcgt cttctcgctg ctcttcatct cggcccccgc gttcttcatc gggaccgtgg
     1021 tcatcatcgg cggcgcgcgc tacgcgccgt ggctgcccac cctgggccac gtgccgttca
     1081 ccgagagccc ggcgcggagc ctcggggcca tgctgcccgc ggcagcctcg ctgggcctgg
     1141 ccatctcggc gatcatcgcc cgcttcacgc ggagcagcat gctcgaggtg ctggggcagg
     1201 acttcgtccg caccgcgcgc gccaagggcg tggccgaccg cgcggtggtc tacaagcacg
     1261 cgctccggaa cgccctgatc ccggtggtcg ccatggccgg cacccagttc gtctacctca
     1321 tcggcggcgc ggtcatcatc gaggaggtct tcgcgctgcc cggcatgggg cgcctcgtcg
     1381 tcaacgccat ctaccagcgg gactacacga tgatccaggg cgcgatcctg ctgctgacgt
     1441 cggcggcggt cctggtcaac ctggccctcg acctcctcta ccacctgatc gaccgccgga
     1501 tcctgtacgc gtgagggacg aatccggcgt gacggggccc cgggtcgggc gccgggcgat
     1561 gcggagcgga tcgctcctgg ccggcgggct catcgtcgcc ggcctcctgg tggccgcgct
     1621 cgccgccccg tggctcgccc cgtacgcgcc caccacgatc ttcccggggg cggagatcca
     1681 gcctcccggc ccgcgcttcc tgctcggcac ggacgaggtg ggccgcgaca tcctgagccg
     1741 cgtgctctat ggcagccgcg tgtcgctcgg ggtcgcgatc ccgtcggtgg cgcttgcggt
     1801 gctgacggga acgacgctcg gcgtgggcct cggctactgg ggcggcgtga ccgacctgct
     1861 cgcgatgcgg atcttcgacg tgctcttcgc cttccccccg atcctcctgg ccatcgccct
     1921 ggtcgcggcc ctcggcccga gcacggtcaa tctcgtgctc accatcgcgg tgctgaccct
     1981 gccccagttc gcggtgctcg cgcgcagcgc caccctcgcc ctcaagccgc aggagttcgt
     2041 gcaggccgcc cgcgccctcg gggcctcgca cgggcgcatc ctgggcgccc acgtcctgcc
     2101 caatctcacg cccaccctcg cggtgcagac gagcctgagc ctctcgatcg tcatcctggt
     2161 ggaggcggcg ctgagcttcc tcgggctcgg cacccagccg ccggcgccga gctggggcgg
     2221 catgctgagc cgcggccgcc agtacatgac catcgccccg tggctggtgc tggcgccggg
     2281 gctggccatc atgctcgccg tgctcggctt caacttgctc ggtgacgccc agcgcgatct
     2341 cctggacccg cgccgggcgg gaaccggccg gaggacccca tgagcccatc cgtgcgcatc
     2401 gcggccgtcc aggcgcggcc tcagtccgac ctcttcgacg acatgtggaa cggtggcgac
     2461 gtggcccacg cggtcgacct gctcgaggcg gcggcccggg ccggcgccgc ctgcgtctgc
     2521 tttcccgagc tgtatccgcg cgtcggcgag gccgagatcc gcgcggcggc gcggcgcctc
     2581 ggtgtcttcg tgatcgccgg cctgatcgag ggcacccggg ccagctggca caatacctcg
     2641 accgtgatcg gacccgacgg ccggatcctc gcgcgacagc cgaagtgctt ccccacccag
     2701 aacgagatcg acaacggcgt ggcggccggc aagggctacc gcgtcgtgga gaccgacatc
     2761 ggccggctgg gcatcgtgat ctgcgccgac ttcgccttct tctccggggg ggtcgagtcc
     2821 ctcgtcgagc agggcgtgga catcatcttc aacccgtcgt ggtggttcgc cctcggcgag
     2881 gcctatcccg ccaccgtcat cggccgccac atgcagtacg gcaagccggt gatcggcgtc
     2941 gacatcgcgg cgtgcgcgct ccggctccgc ggcgcggacg gccggccggt ggagcggttc
     3001 ccccgcgccg gcggctacag cacggtctgc gtggcgccgc ccatcgcctc gctgccggag
     3061 ctggccgcgt ggttccgcac caaggccggc ggcaccaact cggcgcaggg cttcatccag
     3121 agcctgggag aggaggaggg catcctctac gccgacgtcg acatcgcggc ggtccggcga
     3181 ttccccgggt acttctaccg gtccgcgaat ccgtgagccc gcaggacctg ctcgacgagt
     3241 tggtccaggc cgcgcaacgg ctcggggtcg aggtccggtc ggagcccttc gagacgccgg
     3301 ccgcgctggg cggcgggctc tgcgtcgtgc ggggcgcgca cctcgtgctg atcgaccagc
     3361 gcgcccctct ggtggatcgc ctccgcgccc tcgcccgggc cctcggcgat ctggggagcg
     3421 acgccgtcta catggccccg gaagcccgcg agctggtcga aggatc
//
LOCUS       DATMCV010001045         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_721692, whole genome shotgun sequence.
ACCESSION   DATMCV010001045 DATMCV010000000
VERSION     DATMCV010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721692"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..943
                     /locus_tag="VNU03_24020"
     CDS             80..943
                     /locus_tag="VNU03_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP77613.1"
                     /translation="MIHALDHVVVAVRDLDAAVAAYRALLGRAPAWRAEAHGGGATVV
                     TFALANVAVELMTPSGTGPTAERLTAVLDAQGEGLASLAFATGDIERAHRRLARLGLG
                     PEPIADGASTDAETGATRPWRRTRAATAATHGVRLFFLQQAALPPSRPLVPEPAAVSS
                     LDHVVIRTPDPERATALYGARLDLDMRLDRSNPAWRARLLFFRCGDLIVEVAHDLKGG
                     VGDGPDRLWGLSWRVPDAEAARTRLAAAGLDVSEVRPGRKPGTRVLTLRDGTCGVPTL
                     LLERGPAAGDV"
     gene            complement(965..1603)
                     /locus_tag="VNU03_24025"
     CDS             complement(965..1603)
                     /locus_tag="VNU03_24025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77614.1"
                     /translation="MSGPRPTGRRALVLGVPLVLGLLELGHPGVMPGTPIAATLAPIA
                     TWWTVLHVAQVPLFALLGLSVWLLVRDLDGRAARISRSAVAVFVVIYPAFDAAVGVGS
                     GVVLSALGPQSADGMAAIEPGLRALFWGPVTGMMAIVGGGAWVVALLAAAWAWRGAGA
                     PWPIVALLAASGLLLGFSHIRPFGPLACGALLVAAAWIESAGARRRAAPRPA"
     gene            complement(1600..>2400)
                     /locus_tag="VNU03_24030"
     CDS             complement(1600..>2400)
                     /locus_tag="VNU03_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP77615.1"
                     /translation="ESDRADGRVSLPRVTYVGGPTALLEWGGLRLLTDPTFDPAGASF
                     TAGAYQLRKTQGPARPAASLLPVDAVLLSHDHHFDNLDQSGRAFLGQASRVLTTGAGA
                     ERLAHGAVGLLPWQETELTAPDGRVLRVTATPARHGPAHADRGPVIGFVLAFRDDPTT
                     TVYLSGDTVWYPGVGEVAARFPVRLALLFMGAARVAAVGPWHLTFTAEEGVEAARALD
                     AAAILPVHFEGWEHFSESRPEIDRAFAAAGLAGRLRWPVAGVPTDIEG"
BASE COUNT          315 a          934 c          900 g          251 t
ORIGIN      
        1 gtctagcata ccttgacgcc gccgacggcg cggaggcagt atcgcgccga tgaccaccgc
       61 ccccgtagcg tcgccgccga tcatccacgc gctcgaccac gtggtcgtgg cggtgcgcga
      121 tctcgacgcc gcggtggccg cctatcgcgc gctgctcggg cgcgcgcccg cctggcgcgc
      181 cgaggcgcac gggggcggcg cgacggtggt gacgttcgcc ctcgccaacg tcgcggtgga
      241 gctcatgacc ccatccggga cgggaccgac cgccgagcgg ctcaccgccg tcctcgacgc
      301 gcagggcgag ggcctggcga gcctcgcctt cgcgaccggc gatatcgagc gcgcccatcg
      361 ccgcctggcg cggctcggcc tcggccccga gcccatcgcc gacggcgcca gcaccgacgc
      421 cgagaccggc gcgacccggc cgtggcgccg cacgcgcgcg gccaccgcgg ccacccacgg
      481 cgtgcgcctc ttcttcctgc agcaggccgc gctgccgccg tcgcggccgc tcgtgccgga
      541 gccggccgcg gtgagcagcc tcgatcacgt ggtgatccgc acgccggacc cggagcgcgc
      601 caccgcgctc tacggcgcgc ggctcgatct cgacatgcgc ctggaccgct cgaacccggc
      661 gtggcgcgcg cgcctgctct tcttccgctg cggggatctg atcgtggaag tggcgcacga
      721 tctcaagggt ggcgtgggcg acgggcccga tcgcctctgg gggctctcgt ggcgcgtgcc
      781 cgatgcggag gccgcgcgga cgcggctcgc ggccgcgggg ctcgacgtct cggaggtgcg
      841 cccgggccgc aagccgggca cgcgtgtgct gacgctgcgc gacggcacct gcggcgtgcc
      901 gacgctgctg ctcgagcgcg gcccggccgc cggcgacgtc taggccgacg cgggctgcgg
      961 cgctctaggc gggacggggt gcggcccggc gtcgcgcgcc ggccgactcg atccacgccg
     1021 ccgcgaccag cagcgcgccg caggcgagcg gcccgaacgg gcggatgtgg ctgaatccca
     1081 gcaacaggcc cgaggccgcg agcagcgcca cgatcggcca cggcgcgccg gccccgcgcc
     1141 acgcccaggc cgcggccagc agggccacca cccacgctcc cccgcccacg atcgccatca
     1201 tcccggtcac cgggccccag aagagggccc gcagcccggg ctcgatcgcg gccattccgt
     1261 cggcggactg cgggccgaga gcggacagca ccaccccgct ccccacgccg accgcggcgt
     1321 cgaaggccgg gtagatcacg acgaagaccg cgacggcgct gcggctgatc cgggccgcac
     1381 gcccgtccag gtcgcggacc agcagccaca ccgagagccc cagcagggcg aagagcggga
     1441 cctgcgcgac gtgcagcacc gtccaccacg tggcgatcgg ggccagcgtg gccgcaatgg
     1501 gcgtcccggg catcacgccc ggatgtccca gctccagcag cccgagcacg agcggcacgc
     1561 cgaggacgag cgcccgtcgc ccggtcggtc gtggcccgct catccctcga tgtcggtcgg
     1621 cacgcccgcg acgggccacc gcaggcggcc ggccaggccg gcggccgcga aggcccggtc
     1681 gatctcgggg cgcgattcgg agaagtgctc ccaaccctcg aagtggaccg gcaggatggc
     1741 ggccgcatcg agcgcgcggg cggcctcgac gccttcctcg gcggtgaacg tgaggtgcca
     1801 cggccccacc gccgccaccc gcgcggcgcc catgaacagc agcgcgagac ggaccgggaa
     1861 gcgcgcggcg acctcgccga cgcccgggta ccagacggta tcgcctgaga gatagaccgt
     1921 cgtcgtcggg tcgtcccgga acgcgagcac gaatccgatc acgggaccgc ggtcggcgtg
     1981 ggccgggccg tggcgggcgg gcgtcgcggt cacgcggagc acgcggccgt cgggcgcggt
     2041 cagctcggtc tcctgccacg ggagcagccc gaccgcgccg tgggccagcc gctcggcgcc
     2101 ggcgcccgtc gtcagcacgc gcgatgcctg ccccaggaac gcgcggccgg actggtcgag
     2161 gttgtcgaag tggtggtcgt ggctcaggag caccgcgtcc acaggcagca gcgacgccgc
     2221 cggccgcgcc gggccctggg tcttgcgaag ctgataggcg ccggccgtga aggacgcccc
     2281 ggcggggtcg aacgtcggat cggtgaggag gcgcaggccg ccccattcca gcagggcggt
     2341 cggcccgccg acgtaggtga cacgagggag gctcacgcgc ccgtcagccc gatcgctctc
//
LOCUS       DATMCV010001046         2717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_124907, whole genome shotgun sequence.
ACCESSION   DATMCV010001046 DATMCV010000000
VERSION     DATMCV010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124907"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VNU03_24035"
     CDS             complement(<1..383)
                     /locus_tag="VNU03_24035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77616.1"
                     /translation="MKTRAAMRLAGVLLTMSAPIVSGADAQDLEKLAMARVRLTTDAG
                     LTHGCGRIGSVHDDSVRDLRRKIVKMGGNTGLLSFGGSEDLSTVYAEVFRCVGPPASA
                     PGSAPARIPAPPAGPPPAPPPPPAAP"
     gene            421..1788
                     /locus_tag="VNU03_24040"
     CDS             421..1788
                     /locus_tag="VNU03_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWP77617.1"
                     /translation="MIPDRGRPVRLAFRLILALAVLAVVGGAAAQQPVQTPLLLMISI
                     DGLRPDHVTAADGLGLKIPNLRRFLAEGTYAEGVQGVIPTVTYPSHTTLITGVWPARH
                     GILANVAFDPLRENQSGWYWYTEDIRVPTLWDAAARAGLSTASVQWPVSVGARVTWNI
                     PEFWRAGTPDDAKLLRAVSTPGLLAELEADLGPYPRALTTESDEQRGRYAVRILEKKR
                     PNLLTLHLIALDHAEHESGPGSPESLAVLERLDAVVGSLRTAAETVSPGRAYVAIVSD
                     HGFARTTMQLNLTGEFRRAGLFTVNAQGKVSDWQAMPWTAGGSIAVMLKDPADAATLE
                     KVRALLARLAADPANGIDRVLDAAALRDRGGFPGAAFVVGLKPEWHAGSGLNGPLTGK
                     ATVGGMHGHLPDLPDLRASFFLVGPGVPAGRSLGLIDMRDIATTLARRLDLSLPSAEG
                     KVLVP"
     gene            1785..2396
                     /locus_tag="VNU03_24045"
     CDS             1785..2396
                     /locus_tag="VNU03_24045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77618.1"
                     /translation="MRLKTVRTLTLTMVVVVTMVAIASAQVPIRIRGAISKVSGQTLT
                     IAARDNSTVEVRLADTVGVTAVVTVALSDIKTGAFVGAAALPQPDGTFRAQEVLIFPE
                     SGRGTGEGHYPWDLSPGSTMTNATVDAMVERVEGPILTLKHKGGSVTVAVPTDVPIVT
                     FAPGDKAMLLPGAHVFIPAQQQPDGTVTAGRVLVGKDGLVPPM"
     gene            complement(2420..>2717)
                     /locus_tag="VNU03_24050"
     CDS             complement(2420..>2717)
                     /locus_tag="VNU03_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HWP77619.1"
                     /translation="RMLATSAVALLMAGCVVAQPVVVRRPPPPPPVRVEIAPVAPTPA
                     HVWIPGHWGWHREHYVWVPGHWEVPASPGYVWVPGGWMPREGGYVWVEGHWRAR"
BASE COUNT          369 a         1016 c          970 g          362 t
ORIGIN      
        1 ggggccgcgg gaggcggggg cggcgccgga ggcggcccgg ccgggggcgc cgggatccgc
       61 gccggggcgc tgccgggcgc gctcgcgggc ggtcccacgc agcggaacac ctcggcgtag
      121 acggtcgaca ggtcctccga gccgccgaag gacaggagcc cggtgttgcc gcccatcttc
      181 acgatcttgc ggcgcaggtc cctgactgaa tcgtcgtgca ccgacccgat gcgcccacag
      241 ccgtgggtga gcccggcgtc ggtggtgagc cgcacgcgcg ccatcgcgag cttctcgagg
      301 tcctgcgcgt ccgcgccgga tacgatcggc gcgctcatcg tcagcagcac tcccgccagc
      361 ctcatcgccg cccgcgtctt catggccatc tcccggatcg tgctatatgc tgtctccgcc
      421 atgatacccg accgcggtcg cccggtccgg ctcgcgttcc ggctcatcct ggcgctggcc
      481 gtgctcgcgg tggtcggcgg cgcggccgcg cagcagccgg tgcagacccc gctgctgctc
      541 atgatctcga tcgacgggct ccgccccgat cacgtgaccg cggccgacgg tctgggtctc
      601 aagatcccga acctgcgccg cttcctcgcc gaggggacct acgccgaggg cgtgcagggc
      661 gtcattccca ccgtgaccta cccgagccac accacgttga tcaccggggt gtggccggcg
      721 cgacacggca tcctcgccaa cgtggccttc gacccgctgc gcgagaacca gagcggctgg
      781 tactggtaca ccgaggacat ccgggtcccc accctctggg acgccgcggc ccgcgccggg
      841 ctcagcaccg cgagcgtgca gtggccggtg agcgtggggg cccgcgtgac gtggaacatc
      901 ccggagttct ggcgagcggg caccccggac gacgccaagc tgctgcgcgc ggtctcgacc
      961 ccggggctcc tcgccgagct cgaggcggac ctgggcccgt acccgcgcgc gctcacgacg
     1021 gagtcggacg agcagcgcgg gcggtacgcg gtgcgcatcc tcgagaagaa gcggcccaac
     1081 ctgctcaccc tgcacctcat cgcgctcgat cacgccgagc acgagagcgg gcccggcagc
     1141 ccggagagcc tggcggtgct ggagcgcctc gacgcggtcg tcggctcgct gcgcaccgcc
     1201 gcggagaccg tctcgcccgg gcgcgcctac gtggcgatcg tctccgatca cggcttcgcg
     1261 cggaccacga tgcagctcaa cctcaccggg gagttccgtc gcgcgggtct cttcaccgtg
     1321 aacgcccagg gcaaggtctc cgactggcag gcgatgccgt ggaccgcggg cggctccatc
     1381 gcggtgatgc tgaaggatcc ggcggacgcg gccacgctcg agaaggtgcg cgcgctgctc
     1441 gcgcgcctcg cggcggatcc ggccaacggg atcgaccgcg tcctcgacgc ggcggccctg
     1501 cgcgatcgag gcgggttccc cggcgccgcg ttcgtcgtcg ggctcaagcc cgagtggcac
     1561 gccggctcgg gcctgaacgg cccgctcacc ggcaaggcca ccgtcggcgg catgcacggt
     1621 catctccccg atctgcccga cctgcgggcg tcgttcttcc tggtcggccc cggggtgccc
     1681 gcgggccgct cgctcggcct catcgacatg cgcgacatcg cgacgaccct cgcgcgtcgg
     1741 ctggatctct cgctgccctc cgcggaggga aaggtgctcg tgccatgaga ctcaagacgg
     1801 tcaggacgct gacgctgacg atggtcgtgg tggtcacgat ggtcgcgatc gcgagcgccc
     1861 aggtgcccat ccggatccgg ggcgcgatct ccaaggtgtc cggccagacc ctgacgatag
     1921 ccgcgcgcga caactcgacc gtcgaggtgc gcctggccga caccgtcggg gtgaccgcgg
     1981 tggtgacggt ggcgctatcc gacatcaaga ccggcgcctt cgtgggcgcg gccgcgctgc
     2041 cccagccgga cgggaccttc cgcgcccagg aagtgctgat ctttcccgag tcggggcgcg
     2101 gcaccggcga gggccactac ccgtgggatc tctcgcccgg cagcaccatg accaacgcca
     2161 cggtggacgc gatggtcgag cgggtggagg ggccgatcct gaccctcaag cacaagggtg
     2221 gcagcgtgac cgtggcggtg cccaccgacg tgccgatcgt gacgttcgcg cccggggaca
     2281 aggcgatgct cctgcccggc gcgcacgtgt tcatcccagc ccagcagcag cccgacggca
     2341 ccgtgaccgc cggacgcgtg ctcgtgggga aggacgggct cgtccccccg atgtaggccg
     2401 actcaagggc gccgcgtctt cagcgcgcgc gccagtgccc ctcgacccag acgtacccgc
     2461 cctcgcgcgg catccacccg cccggcaccc atacgtagcc gggggaagcc ggcacttccc
     2521 agtggccggg gacccacacg tagtgctccc ggtgccagcc ccagtgtccg gggatccaca
     2581 cgtgggccgg ggtcggcgcc accggcgcga tctccacgcg gacgggcggg ggaggcggcg
     2641 gccgcctcac gaccaccggc tgcgcgacga cgcagccggc catgagcagc gcgacggccg
     2701 aggtggccag catccgg
//
LOCUS       DATMCV010001047         2473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_54532, whole genome shotgun sequence.
ACCESSION   DATMCV010001047 DATMCV010000000
VERSION     DATMCV010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54532"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1107
                     /gene="pdxS"
                     /locus_tag="VNU03_24055"
     CDS             154..1107
                     /gene="pdxS"
                     /locus_tag="VNU03_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWP77620.1"
                     /translation="MSTVNADNPVGPGGRPPQAPSRPESATGTGRVKRGMAEMLKGGV
                     IMDVVTSDQAKIAEDAGAVAVMALERVPADIRAQGGVARMSDPDMIDGIIAAVSIPVM
                     AKCRIGHFAEAQVLQAIGVDYIDESEVLTPADEAHHVDKWKFTVPFVCGATNLGEALR
                     RIAEGAAMIRSKGEAGTGNVVEATRHMRSIRSEIARLSGLPTEELFTAAKELGAPYEL
                     VAEVARAGRLPVVLFTAGGIATPADAALMMQLGAEGVFVGSGIFKSGDPAKRAAAIVK
                     ATTFHDDPDVIAKVSRGLGEAMVGINLDTLPADQHYAARGW"
     gene            1300..1905
                     /gene="pdxT"
                     /locus_tag="VNU03_24060"
     CDS             1300..1905
                     /gene="pdxT"
                     /locus_tag="VNU03_24060"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWP77621.1"
                     /translation="MTARVIGVLALQGDVPEHLRALEDVGARAVPVRRPEELHQLDGL
                     VIPGGESTTLWRLSVAFDMLEPLRKLIASGLPAFGSCAGMIMLADRLADGVAGQLTYG
                     GIDMSVRRNAFGRQVDSFESDITLSGLDGSDAMPLRAVFIRAPWVEQNGENVTVLGTE
                     QRTGRIVAVRQGQLLATAFHPELTPDRRIHELFVDIVKDNT"
     gene            1902..>2473
                     /locus_tag="VNU03_24065"
     CDS             1902..>2473
                     /locus_tag="VNU03_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWP77622.1"
                     /translation="MSGHSKWATTKHKKAVVDAKRGKLFAKLIKNVEVAARTGGGDPA
                     GNPTLYDAIQKAKKSSVPNDNIDRALRRGSGAEAGGAQYENITYEGYASGGVAVLVEC
                     LTDNRNRAASDVRTAFSRNGGSMADPGSVSYLFHRKGVVVVEKKGVTEDDVLTAVLDA
                     GAEEVNDLGESFEVVSEPADLVATRTALQDA"
BASE COUNT          400 a          838 c          878 g          357 t
ORIGIN      
        1 ctggcccgcg tgctgcacct gtccggccac gcgcaggccc cggtgttctt cgagatggac
       61 gactccctca tcgcggacaa gcccggcgcc tggatgggcc cggctggcca tacgatggtg
      121 tgaagtagtc ctgattgatc aagcgaggtt cgagtgtcca ctgtcaacgc tgataacccc
      181 gttggcccgg ggggacgacc cccccaggcc ccctcgcggc ccgagtcggc cacggggacc
      241 ggacgggtga agcgcggcat ggcggagatg ctcaagggcg gcgtgatcat ggacgtggtc
      301 acgtccgacc aggccaagat cgccgaggat gccggcgcgg tcgcggtgat ggcgcttgag
      361 cgcgtgcccg ccgacatccg cgcccagggc ggcgtggccc ggatgagtga ccccgacatg
      421 atcgacggca tcatcgccgc ggtgtccatc ccggtcatgg ccaagtgccg gatcgggcac
      481 ttcgccgagg cgcaggtcct gcaggccatc ggggtcgact acatcgacga gtccgaggtg
      541 ctcaccccgg ccgacgaggc ccatcacgtc gacaagtgga agttcaccgt gccgttcgtg
      601 tgcggcgcca ccaacctggg cgaggcgctg cggcggatcg ccgagggcgc ggccatgatc
      661 cgctccaagg gcgaggccgg gacgggcaac gtggtcgagg cgacccggca catgcggtcc
      721 atccggtccg agatcgcccg gctgtccggc ttgccgaccg aggagctgtt caccgcggcc
      781 aaggagctcg gcgcgccgta cgagctggtg gccgaggtgg cccgggcggg gcggctgccg
      841 gtcgtgctgt ttaccgcggg cggcatcgcg accccggcgg acgccgcgct gatgatgcaa
      901 ctcggcgcgg agggcgtgtt cgtgggctcg ggcatcttca agtccggcga cccggccaag
      961 cgcgccgccg cgatcgtgaa ggccaccacg ttccacgacg acccggacgt gatagccaag
     1021 gtctcccgcg gcctgggcga ggccatggtc ggcatcaacc tggacaccct ccccgccgac
     1081 cagcactacg ccgcccgcgg ctggtaaccc cgcgccgcgc atcccgcact tcacccgccc
     1141 catcaccacc cgccaaagcg aatgaaaccg gggttccact tcgctctggc gcagtgaaac
     1201 ccgggtttca cttcaaccag ggagcctggc ccgggccgcc ggaaccggga agaagagcag
     1261 cggtttcggt gttttaacgg ggttacgtct ggaggggtaa tgaccgcacg ggttatcgga
     1321 gtgctcgcct tgcagggtga cgtgcccgag cacctgcgcg cgctcgagga cgtcggtgcc
     1381 cgagcggtgc cggtgcgccg ccccgaggag ctccatcagc tcgacggcct ggtaatcccg
     1441 ggcggcgagt cgaccacgct gtggcggttg tccgtcgcgt tcgacatgct cgagccgctg
     1501 cgcaagctga tcgcttccgg cctgcccgcg ttcgggtcct gcgccgggat gatcatgctg
     1561 gccgaccggc tcgccgacgg cgtggccggg cagctgacct acggcgggat cgacatgagc
     1621 gtgcgccgga acgcgttcgg ccgtcaggtc gactcgttcg agagcgacat cacgctgtcc
     1681 ggcctggacg gaagcgacgc catgccgctt cgggcggtct ttatccgggc cccttgggtg
     1741 gagcagaatg gcgagaacgt caccgtcctg ggcaccgagc agcgcaccgg taggatcgtc
     1801 gcggtccggc aggggcaact gctggccacg gccttccacc ccgagctgac cccggaccgg
     1861 cgtatccatg agctattcgt agacattgtg aaggacaaca catgagcggc cactccaagt
     1921 gggccaccac caagcacaag aaggcggtgg tcgacgctaa gcgcggcaag ctgttcgcca
     1981 agctgatcaa gaacgttgag gtcgccgcgc gcaccggcgg cggagatccg gcggggaacc
     2041 cgacgctgta cgacgccatc cagaaggcga agaagagctc ggtccccaac gacaacatcg
     2101 accgcgccct gcgccggggc agcggcgccg aggcgggcgg tgcgcagtac gagaacatca
     2161 cctacgaggg ttacgcctcc ggtggcgtcg cggtgctggt ggagtgcctg accgacaacc
     2221 ggaaccgggc cgcctcggac gtgcgcaccg cgttcagtcg caacggcggc tcgatggccg
     2281 accccgggtc ggtctcttac ctgttccacc gcaagggggt cgtcgtcgtc gagaagaagg
     2341 gcgtgaccga ggacgacgtg ctcaccgccg tgctcgacgc gggcgccgag gaggtcaacg
     2401 acctcggcga gtcgttcgag gtcgtgtccg agccggccga cctggtggcg acccgcacgg
     2461 cgctgcagga cgc
//
LOCUS       DATMCV010001048         3769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_461809, whole genome shotgun sequence.
ACCESSION   DATMCV010001048 DATMCV010000000
VERSION     DATMCV010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461809"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2812
                     /locus_tag="VNU03_24070"
     CDS             <1..2812
                     /locus_tag="VNU03_24070"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWP77623.1"
                     /translation="GAPVRDPHGLVVGAVLVLRDLTERRSIERDQAAVIAREQDARRD
                     AAALSAVGRALVQSLDGASVGRHIAEGIRTLLGGTVSVLWELDPASGRLVAVAASGAS
                     GVFSPGTVIPGDELVAGLAVREGAPVSTPDVLADLRVVVNAETRAMVERAGHGAVLAV
                     PLAIGGRVTGVLAVGDVTGRTFGPEEIGRLQDFANQAAIALENARLFALETARRAHLE
                     ALAAVQRDLSAELDLDRLLSLIIERAGRLFEGGCFAYLVDEEGHTLGSRAWSRSEPAQ
                     EAPFPEGGGLVASCAVARHGLLANDYPRSPYALPAVVRLGVHHAMAVPLVSRGRLLGV
                     LVLARRETGPPFSREDLAAFEGLSVQAAVAVDNAALFVEAERRRREAEVLAELARSIN
                     AAHDVSTVLQRVVDGAKELCGCDLTSVALRETDSGAIVMRNRAGEYRGLQDRLVIEPG
                     RGAGGLVLQSGEPFRSDDIGRDSRISPEQDRLIEIEGLVATLIVPIMVEGRVEGLLYV
                     HRRTPTPFTDRSETILRLLAEYASIAIHNMRLLAHEHQMRAEAEGASRMKDEFLATIS
                     HELRSPLQPLLNWAYLLRSPNLDPASAERALDAIERSTKTLGQLIEDLLDVSRIVTGK
                     LRLQARPVRLPGVIRSAIEAVEAAALAKAVVLEARLDPDLPLVLGDPDRLQQVLWNLL
                     SNGIKFTPRGGRVMVTVTGREGEVLITVADTGAGIKPEFLPHVFERFRQAESSTNRAY
                     GGLGLGLAIVRHLVELHGGSVSVASEGEGRGATFTARLPVAAAVRLPAERPPAAVTSD
                     TPAGPNLAGLHILVVDDEADAREVMRFMLERGGARVRIADSAAAALDAVREERPDLLI
                     SDIGMPIEDGYVMVRRLRAMEDGVRRRLPAIALTAYASDEDSRRAHAAGFDAHLSKPV
                     DPARLIEIAVGLAGPRRVAERG"
     gene            complement(2845..3627)
                     /locus_tag="VNU03_24075"
     CDS             complement(2845..3627)
                     /locus_tag="VNU03_24075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77624.1"
                     /translation="MSALAGTLLTLGASPALAAPVTVRYLEGVTHGFLVLRGPGGETL
                     AEGDLLQVARPEGVDSRLTFRFRDGSLYDETVVFTQQKVFALQSYHLIQRGPTFPEEL
                     EVTFDRKGGRYQVRSRRDGGEAETTAGEVELPPDLYNGMMIMLLRNLGEGAAETVHVL
                     AFTPKPTLVQLEMVPVGEDTLRAGERRVQATHYVMTPKLGMLRGAAAAVLGKTPAPYH
                     CWVVTAGAPAFVAIDGPLYTGGPIWRIETVSPAGPPRPAPAR"
BASE COUNT          491 a         1359 c         1430 g          489 t
ORIGIN      
        1 cggcgccccg gtgcgcgacc cgcacggact ggtggtcggc gcggtgctgg tcctgcgcga
       61 tctcaccgag cggcggagca tcgagcgcga ccaggccgcg gtgatcgcgc gcgagcagga
      121 cgcgcgtcgc gacgcggccg cgctgtccgc ggtggggcgg gccctcgtgc agtccctcga
      181 cggcgccagc gtgggccggc acatcgcgga agggatccgg acgctgctgg gcggcaccgt
      241 gtccgtcctg tgggagctcg acccggcctc ggggcgcctc gtggcggtcg ccgcctccgg
      301 cgcgagcggg gtcttctcgc cgggcaccgt cattccgggc gacgagctgg tggccgggct
      361 cgcggtgcgc gagggagccc cggtctcgac gccggacgtc ctggccgatc tgcgcgtggt
      421 ggtgaacgcg gagacgcggg ccatggtcga gcgcgccggg cacggcgcgg tgctcgcggt
      481 gccgctcgcc atcggcggcc gggtgaccgg ggtcctcgcg gtcggcgacg tcaccggccg
      541 caccttcggc cccgaggaga tcgggcggct gcaggacttc gccaaccagg ccgcgatcgc
      601 gctggagaac gcgcggctgt tcgcgctcga gaccgcccgg cgcgcgcatc tcgaggccct
      661 cgcggcggtg cagcgcgacc tgagcgcgga gctcgatctc gaccggctgc tgagcctgat
      721 catcgagcgg gcgggccggc tcttcgaggg cggctgcttc gcctacctgg tggacgagga
      781 gggccacacg ctcggcagcc gcgcctggag ccggagcgag ccggcgcagg aggccccgtt
      841 cccggaaggg ggcgggctcg tggcgagctg cgcggtggcc cggcacggcc tgctcgccaa
      901 cgactacccg cgctcgccct acgcgctgcc cgcggtggtg cgcctcgggg tccaccacgc
      961 gatggcggtg cccctggtga gccgcggtcg cctgctcggc gtcctggtgc tggcgcggcg
     1021 cgagaccggg ccgccgttct cccgggaaga cctcgccgcc ttcgaggggc tctcggtcca
     1081 ggccgcggtg gcggtggaca acgcggcgct cttcgtcgag gcggagcgcc gccggcggga
     1141 ggcggaggtg ctggcggagc tggcgcgatc catcaacgcg gcgcacgacg tgagcaccgt
     1201 gctccagcgg gtggtcgacg gggccaagga gctctgcggg tgcgacctca ccagcgtggc
     1261 cctgcgcgag accgacagcg gcgcgatcgt catgcgcaac cgggccgggg agtaccgcgg
     1321 cctccaggat cgtctggtca tcgagcccgg ccgcggcgcg ggcggcctcg tgctgcagtc
     1381 gggcgagccc tttcgcagcg acgacatcgg gcgggactcc cggatcagcc cggagcagga
     1441 ccggctgatc gagatcgagg gtctcgtcgc caccctgatc gtgccgatca tggtggaggg
     1501 ccgggtggag ggtctcctct acgtgcaccg gcgcacgccg acccccttca ccgaccgcag
     1561 cgagaccatc ctccgcctgc tcgcggagta cgcgagcatc gcgatccaca acatgcggct
     1621 gctcgcgcac gagcaccaga tgcgggccga ggccgaaggc gcgagccgga tgaaggacga
     1681 gttcctcgcc accatctccc acgagctgcg cagcccgctg cagccgctgc tcaactgggc
     1741 gtacctcctg cgctcgccca acctcgaccc cgccagcgcg gagcgcgcgc tcgacgccat
     1801 cgagcggagc accaagaccc tcgggcagct catcgaggat ctcctcgacg tctcccgcat
     1861 cgtcaccggc aagctgcgcc tgcaggcgcg gccggtgcgc ctgcccgggg tgatccgctc
     1921 cgccatcgag gcggtggagg cggccgcgct cgcgaaggcg gtggtcctcg aggcgcggct
     1981 cgatccggac ctcccgctgg tgctcggcga tccggatcgc ctccagcagg tactgtggaa
     2041 cctcctgtcc aacggcatca agttcacgcc ccgcggcggc cgcgtgatgg tgacggtgac
     2101 cgggcgcgag ggggaggtgc tgatcacggt ggccgacacc ggggccggca tcaagcccga
     2161 gttcctcccg cacgtgttcg agcgcttccg tcaggcggag agcagcacga accgcgcgta
     2221 cggcgggctc gggctcggac tcgcgatcgt tcgccacctg gtcgagctgc acggggggag
     2281 cgtctcggtg gcgagcgagg gcgagggccg gggggcgacc ttcaccgcgc ggctgccggt
     2341 ggcggccgcg gtgcgactgc ccgccgagcg cccgcccgcc gcggtcacca gcgatacgcc
     2401 ggccgggccg aacctcgccg gcctgcacat cctggtggtc gacgacgagg ccgacgcccg
     2461 cgaggtgatg cgcttcatgc tcgagcgcgg cggcgcccgc gtccgcatcg ccgactcggc
     2521 ggcggccgcc ctcgacgcgg tccgggagga gcggcccgac ctgctcatca gcgacatcgg
     2581 catgccgatc gaggacgggt acgtgatggt gcggcggctc cgcgcgatgg aggacggggt
     2641 gcgacggcgg ctgcccgcga tcgcgctcac cgcgtacgcg agcgacgagg attcgcggcg
     2701 ggctcacgcg gccggcttcg acgcccacct ctcgaagccg gtggatccgg cccggctcat
     2761 cgagatcgcg gtcggcctcg cgggcccacg ccgcgtggcg gagcgcgggt agatcactcc
     2821 acgcgctcgc cgggcccgcg ccggtcagcg cgccggtgcg ggacggggcg gcccggccgg
     2881 gctcaccgtc tcgatgcgcc agattggtcc gccggtgtag agcgggccgt cgatggccac
     2941 gaaggccggc gcccccgccg tcacgaccca gcagtggtag ggggcgggcg tcttgccgag
     3001 cacggccgcc gccgccccac gcagcatccc gagcttcggg gtcatgacgt agtgggtcgc
     3061 ctggacccgg cgctcgccgg cccgcaacgt atcctcgccg accgggacca tctcgagctg
     3121 cacgagggtg ggcttcgggg tgaaggccag cacgtggacg gtttctgccg cgccctcgcc
     3181 caggttgcgg aggagcatga tcatcatgcc gttgtacaga tcgggcggga gctcgacctc
     3241 gcccgccgtg gtctccgcct cgccgccgtc gcgacgtgag cgcacctggt agcggccacc
     3301 cttgcggtcg aaggtgacct cgagctcctc cgggaaggtg ggcccgcgct ggatcaggtg
     3361 gtagctctgc agggcgaaga ccttctgctg ggtgaagacc accgtctcgt cgtagagcga
     3421 gccgtcgcgg aagcggaagg tcagccgcga gtccacgccc tctggccggg ccacctgcag
     3481 cagatcgcct tccgcgaggg tctcgccacc cgggccgcgc agcaccagga agccgtgggt
     3541 cacgccctcg agatagcgca ccgtcaccgg cgccgcgagc gccggggacg ctcccaacgt
     3601 cagcagcgtg ccggcgaggg cactcaccac gaagatgtcg ccgagcaaac gaacgagccg
     3661 ctgccggcgc atgactggag agagtgcaag cgcgctgcca tcggccggtc gcgcaagtac
     3721 ccaggtccac tcagggctcg cgcgtgaatc atcccgacgc gggccggag
//
LOCUS       DATMCV010001049         4598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_856122, whole genome shotgun sequence.
ACCESSION   DATMCV010001049 DATMCV010000000
VERSION     DATMCV010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856122"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VNU03_24080"
     CDS             complement(<1..298)
                     /locus_tag="VNU03_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77625.1"
                     /translation="MSGGSLGALTLTGSVKALALELGFDLVAVGPATPPEHGAALRRW
                     VEAGHAGTMGYLERRLEERLDPDRVLPGARTAVCVALNYYQGEPVDPSWRPIARY"
     gene            complement(295..945)
                     /locus_tag="VNU03_24085"
     CDS             complement(295..945)
                     /locus_tag="VNU03_24085"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWP77626.1"
                     /translation="MPSLIQAAGVSPRFLDLLGGEPLAGHRVLDVGCGAGRLSLALAP
                     VAGWVVGLDRDAPALEEARRRAAAGGLANVEFHEADVEATPYEPWRPDLVTAHLCASD
                     AIIERAAAALRPGTCLAMVAFHVDQWKETGRVSRFAYDEARMRAALEEHGFTVEALEV
                     EQEVRRFASVEEGLAAAVGLEDRWRTDGRWFRYIAFLESGGRTLTRSHLLVKARRA"
     gene            complement(958..4413)
                     /gene="metH"
                     /locus_tag="VNU03_24090"
     CDS             complement(958..4413)
                     /gene="metH"
                     /locus_tag="VNU03_24090"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWP77627.1"
                     /translation="MALPERSARLAQELRRRILVLDGAMGTMIQARALSAADFGGPQL
                     EGCNENLNLTRPDVITDIHAAYLDAGADLISTNTFGCAPYVLAEYGLSERCHEITLAA
                     ARLCRAAVDRASTSERPRFAVGAMGPGTRTITVTANVTFDEVRDAYYRQARALIEGRV
                     DALLLETCQDTLNVKAAAIGIKQALTEAGVELPLMISGTVEPMGTMLAGQGVDAFYAS
                     IEHLAPFSVGLNCSTGPEFMTDHLRTLAELATCFVSVYPNAGLPDEHGHYEETAESLA
                     LKMRRFVDEGWVNVVGGCCGTTPAHIRELARLVAERPPRAPATDLAPAVSGVEAVYPA
                     DDVRPIIVGERTNVIGSRRFKELIVEEKFEEASEIGRAQVRGGAQVLDVCLANPDRDE
                     AADIDRFMDFVTRKVKAPLMIDSTDAHVLELALMKCQGKAIVNSINLEDGEERFERVV
                     PLIRAYGGAVVVGCIDEDKQQGMAVTRQRKLAVAERSHALLTGKYGLPERDLIFDPLV
                     FPVGTGDANYIGSAVETIEGVRAIKARFPRAKTILGISNVSFGLPTAGREVLNAVFLY
                     ECTKAGLDYAIVNSERLERYASIPDEERRLAQDLIYWRGSDPVAAFAAHFRGRTRAPK
                     DTGVKLPLDERLARYIVEGSKDGLLDDLALKLKEQTALEIVNGPLMRGMEEVGRLFND
                     NQLIVAEVLQSAEAMKASVAYLEQFMEKDETAARGTIVLATVKGDVHDIGKNLVEIIL
                     KNNGYRIVNLGIKVPPEDLIAAYHSHKPDAIGLSGLLVKSAQQMVVTAQDLRAAGIEV
                     PLFVGGAALTRKFTATRIAREYTGLTLYAKDAMDGLDLANRLFSATTREALVDRLRAE
                     QEALRAGADAADAAPARAPAVGPSRSAVSRTVPVPAPPDLALHVLRDVPLTHVFPYLN
                     LQMLLGKHLGVKGSVARLLEQRDPKVLEINQVVEDLERLAVEQHVIRASGLYRFYEAA
                     AAGDDLVLMDGGREAARFHFPRQADGERLCLSDYVREQGSGEKDYVALFAVTCGAGVR
                     DLAERWKEEGQYLRSHALQALAIECAEAFAEMLHARLRTQWGFPDPPGLELSEKLKAH
                     YRGLRVSFGYPACPNLADQRILFDLLEPGQIGLGLTDGFMMDPEASVSALVFHHPEAK
                     YFKAE"
BASE COUNT          627 a         1583 c         1718 g          670 t
ORIGIN      
        1 cgtagcgcgc gatcgggcgc caggagggat cgacgggctc gccctggtag tagttgagcg
       61 ccacgcacac ggccgtgcgc gcgcccggca gcacccggtc cggatcgagc cgctcctcga
      121 gccgccgctc gaggtagccc atggtgccgg cgtggcccgc ctcgacccag cgcctcagcg
      181 ccgcgccgtg ctcgggcggc gtggccgggc ccaccgcgac gaggtcgaag cccagctcga
      241 gggcgagggc cttcaccgac ccggtgagcg tcagcgcccc caggctcccg ccgctcacgc
      301 gcgccgcgcc ttgaccagca ggtggctgcg cgtgagcgtg cggccgccgg actcgagaaa
      361 ggcgatgtag cggaaccagc ggccgtcggt tcgccaccgg tcctccaggc cgaccgcggc
      421 ggccaggccc tcctcgacgg atgcgaagcg ccgcacctcc tgctcgacct cgagcgcctc
      481 gacggtgaag ccgtgctctt cgagcgccgc gcgcatgcgt gcctcgtcgt aggcgaagcg
      541 ggagacgcgg ccggtctcct tccactggtc cacgtggaag gcgaccatgg ccaggcaggt
      601 gccggggcgg agcgcggcgg ccgcgcgctc gatgatcgcg tccgaggcgc agaggtgcgc
      661 ggtcaccaga tctgggcgcc agggctcgta gggggtggcc tcgacgtcgg cctcgtggaa
      721 ctccacgttg gcgagcccgc ccgcggcggc ccgtcgccgg gcctcctcga gcgcgggggc
      781 gtcgcggtcg aggccgacga cccagcccgc gaccggggcc agcgcgaggg agagccggcc
      841 cgcgccgcag ccgacgtcga gcacccggtg cccggctagc ggctcaccgc cgaggaggtc
      901 gagaaagcgg gggctgacgc cggccgcctg gatcaggctc ggcatgcggc gcctcgccta
      961 ctcggccttg aagtacttgg cctcgggatg gtggaagacc agcgcggaga ccgaggcctc
     1021 cggatccatc atgaagccgt cggtcaggcc gagtccgatc tgcccgggct cgagcaggtc
     1081 gaacaggatg cgctggtcgg cgaggttcgg gcacgccggg tagccgaagg agacgcggag
     1141 gccacggtag tgggccttga gcttctcgga gagctcgagg ccgggcggat ccgggaagcc
     1201 ccactgggtg cgcagccggg cgtggagcat ctccgcgaac gcctccgcgc actcgatcgc
     1261 cagggcctgg agcgcgtggg agcggaggta ctggccctcc tccttccacc gctcggccag
     1321 gtcgcggacg ccggcgccgc aggtcaccgc gaagagcgcg acgtagtcct tctccccgct
     1381 cccctgctcg cgcacatagt cggataggca gagccgctcg ccgtccgcct ggcgcggaaa
     1441 gtggaagcgc gcggcctcgc ggccgccgtc catgagcacc aggtcgtccc ctgcggcggc
     1501 cgcctcgtag aagcggtaga ggccgctcgc gcggatgacg tgctgttcga cggccaggcg
     1561 ctcgaggtcc tccaccacct ggttgatctc gagcaccttc ggatcgcgct gctcgagcag
     1621 ccgcgccacc gagcccttca ccccgaggtg cttgccgagg agcatctgga ggttcaggta
     1681 cgggaagacg tgggtgagcg gcacgtcgcg caggacgtgg agcgccagat cgggcggcgc
     1741 gggcaccggc accgtgcgcg acaccgcgga gcggctcggg ccgaccgcag gagcgcgggc
     1801 cggggccgcg tcggccgcgt cggcgccggc ccgcagggcc tcctgctcgg cgcgcaggcg
     1861 gtcgacgagg gcctcgcggg tggtggcgct gaagaggcga ttggccaggt cgaggccgtc
     1921 catcgcgtcc ttggcgtaga gggtgagccc ggtgtactcg cgggcgatgc gggtcgcggt
     1981 gaacttgcgg gtgagcgcgg cgccgcccac gaagagcggg acctcgatgc cggccgcgcg
     2041 caggtcctgg gcggtgacca ccatctgctg ggccgatttc accaggaggc cggacaggcc
     2101 gatcgcgtcg ggcttgtggc tgtggtacgc ggcgatgaga tcctcgggcg gcaccttgat
     2161 cccgaggttc acgatccggt agccattgtt cttcaggatg atctcgacca ggttcttacc
     2221 gatgtcgtgg acgtcgccct tcacggtggc cagcacgatg gtgccgcgcg ccgcggtctc
     2281 gtccttctcc atgaactgct cgaggtaggc cacggaggct ttcatggcct cggcggactg
     2341 cagcacctcg gccacgatga gctggttgtc gttgaatagg cgcccgacct cctccatgcc
     2401 ccgcatgagc gggccgttga cgatctccag agcggtctgc tccttgagct tcagcgcgag
     2461 gtcgtcgagc aggccgtcct tggagccctc cacgatgtag cgggcgagcc gctcgtccag
     2521 cgggagcttc accccggtgt ccttcggggc ccgggtgcgc ccccggaagt gcgccgcgaa
     2581 ggcggcgacg ggatcgctgc cccgccagta gatcaggtcc tgggccaggc ggcgctcctc
     2641 gtcggggatc gaggcgtagc gctcgagccg ctcgctgttg acgatggcgt agtcgaggcc
     2701 ggccttggtg cactcgtaga ggaagaccgc gttcagcacc tcgcgccccg cggtgggcag
     2761 cccgaacgag acgttggaga tgccgaggat ggtcttggcg cgcgggaagc gggccttgat
     2821 cgcccgcacc ccctcgatgg tctcgaccgc ggagccgatg tagttggcgt cgccggtgcc
     2881 caccgggaag accagggggt cgaagatcag gtcgcgctcg ggcaggccgt acttgccggt
     2941 gagcagggcg tgcgagcgct ccgcgacggc aagcttgcgc tggcgggtca ccgccatgcc
     3001 ctgctgcttg tcctcgtcga tgcagcccac caccaccgcg ccgccgtagg cccggatgag
     3061 cggcaccacc cgctcgaagc gctcctcgcc gtcctcgaga ttgatcgagt tgacgatggc
     3121 cttgccctgg cacttcatca gggccagctc cagcacgtgc gcgtcggtcg agtcgatcat
     3181 cagcggggcc ttgaccttgc gggtgacgaa gtccatgaag cggtcgatgt cggccgcttc
     3241 gtcccggtcc gggttggcca ggcagacgtc gagcacctgc gctccccccc ggacctgggc
     3301 gcggccgatc tcggaggcct cctcgaactt ctcctcgacg atcagctcct tgaagcgccg
     3361 cgagccgatc acgttggtcc gctcgcccac gatgatcggc cgcacgtcgt ccgccgggta
     3421 gaccgcctcg acgccgctca ccgcgggggc gagatccgtg gccggcgcgc gcggggggcg
     3481 ctcggccacc aggcgggcca gctcgcggat gtgggccggg gtggtgccgc agcagccacc
     3541 gaccacgttg acccagccct cgtccacgaa gcgccgcatc ttgagcgcga ggctctccgc
     3601 ggtctcttca taatggccgt gctcgtcggg cagccccgcg ttcgggtaga ccgagacgaa
     3661 gcaggtggcc agctccgcga gggtgcgcag gtggtcggtc atgaactcgg gcccggtcga
     3721 gcagttgagg ccgaccgaga acggggcgag gtgctcgatc gaggcgtaga aggcgtcgac
     3781 gccctgcccg gcgagcatgg tgcccatcgg ctcgacggtg ccgctgatca tgaggggcag
     3841 ctcgaccccg gcctcggtca gggcctgctt gatcccgatc gcggccgcct tgacgttcag
     3901 ggtgtcctgg caggtctcga gcaggagcgc gtcgacccgg ccctcgatga gggcgcgcgc
     3961 ctggcggtag taggcgtcgc gcacctcgtc gaaggtcacg ttggcggtca ccgtgatcgt
     4021 ccgcgtcccc gggcccatgg cgccgacggc gaagcgcggg cgctcggagg tggaggcccg
     4081 gtcgaccgcg gcccggcaga gtcgcgccgc ggccagcgtg atctcgtggc accgctcgga
     4141 gagcccatac tcggcgagga cgtagggcgc gcagccgaag gtgttggtcg agatcaggtc
     4201 cgcgcccgcg tcgaggtagg ccgcgtggat gtcggtgatg acgtccgggc gcgtgaggtt
     4261 caggttctcg ttacagccct cgagctgggg tcccccgaag tcggccgccg agagggcgcg
     4321 ggcctggatc atggtgccca tcgcgccgtc caggaccagg atgcggcgac gcagctcctg
     4381 ggccagacgt gcagaccgtt cgggaagagc catgctgtcg attgtagcgc atgcgcgctc
     4441 gggcccgcgc gtgccgactc agccgcgctt cttgcgcccg cggaggtcga ggaccccggg
     4501 gcgggcgcgc gggtcctcgc cccgggggaa gatctgggcc ttctggacct tctgggtgcc
     4561 ggtggtgggc agtcgctcca cgaagagcac ccagccgg
//
LOCUS       DATMCV010001050         4885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_790101, whole genome shotgun sequence.
ACCESSION   DATMCV010001050 DATMCV010000000
VERSION     DATMCV010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790101"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VNU03_24095"
     CDS             complement(<1..1051)
                     /locus_tag="VNU03_24095"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP77628.1"
                     /translation="MLSREDNELLTRTGPGTPMGDLVRRYWIPVVESKDLAPGGRVKR
                     VQLLGERLIAFRGKSGRAGLIGEFCPHRSASLFYGRVEDAGMRCAYHGWKFGLDGQCE
                     EMPSEPPESGFASKVKTSAYPCAEAGGVVFAYMGPESTPPPLPELEWTLLPEGHTFTS
                     RRVQDCNWFQALEGGIDSSHISFLHAPIDHRNSEVTQDMDRVSFGVGAAVSTGDRAPR
                     FEVVDTDYGVLIGARRANPDGRWYWRVTQFLLPFYTMPPTDLDERVVQSHIWVPMDDT
                     HVVNWMVTWHPDRKLTPEEIQLHVEGKGAHVCDYAPATSEPYGDVRTAANRENDYFMD
                     WDVHRTRMYCGIPGFG"
     gene            1177..1941
                     /locus_tag="VNU03_24100"
     CDS             1177..1941
                     /locus_tag="VNU03_24100"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWP77629.1"
                     /translation="MLASAVDLTLDGPVARITLNRPEVLNAGDPRWVSELAAAVAAIA
                     ARSDLRVAVIGGAGRAFCTGVDLKALAAGAMTLPHFVAWEDAMTAIERLPLPFIAAIN
                     GHCLGGGLQLALVCDYRLAAAEATIGLPAVKECLIPSMALYRLPRLIGDARARELILT
                     GEPITAARAEQYGLVHRVVPGADFARALEESVARFLALPATSVAWSKRLMARAFDLGF
                     DQFRREMEAGFRECLDSAEHRAAMEDIRAGMEKRSR"
     gene            1938..2960
                     /locus_tag="VNU03_24105"
     CDS             1938..2960
                     /locus_tag="VNU03_24105"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWP77630.1"
                     /translation="MTAARPLLPTSVVGSHGLPGWVWLAREAIQAGRLGATDLAELME
                     DATQVALLDQERAGVDVLSTGEMGRVRFIIGFYHHLQGIRALPEPRLLGQPHWDTNTP
                     FQVIEKVSAPAGLGIVDEFKLARSLTTRPLKATVPGPYTLMFPLRPGGPYRDRDTLLA
                     DLVAIVNAECRALVDAGADFIQIDEPHSGMYAGSTPQFALGVNRAVEGVRAKIAVHVC
                     FGNLYGRPFQPVRSYRNVYPALHELTASQIVLEYANRGMDDLPLWTEFPTDKELGAGV
                     IDVKAFKAETAAEVAERIRALLRHVAPDKLWLNPDCGFWETPRWATRLKLAALVEGAR
                     LVRREL"
     gene            complement(2972..3751)
                     /locus_tag="VNU03_24110"
     CDS             complement(2972..3751)
                     /locus_tag="VNU03_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77631.1"
                     /translation="MAADMAKGFAVFAGVAPEIIRASAREAEARGFTSFWVNHPGPVD
                     GLGSLAQGGAETQRIDLGVGVIPLHTRGPEAIVAGVRQHGLAPARLLLGVGSPNPQAL
                     ARVRDGVAAIRKSLTTRIVVAALGPKMCELAGQVADGVLFNWLTPEYARRSAELVRAG
                     AKAAGRPMPRTYAYVRVALGPGAADRLSDEGGRYAAIPAYGAHFVRMGVKPVDTAIAA
                     TSPEGLKTALAAWRGAVDEVVLRAIVAHDTVEDHLTLLRAI"
     gene            3874..4885
                     /locus_tag="VNU03_24115"
                     /pseudo
     CDS             3874..4885
                     /locus_tag="VNU03_24115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          621 a         1868 c         1677 g          719 t
ORIGIN      
        1 ccccgaagcc ggggatcccg cagtacatcc gggtgcggtg gacgtcccag tccatgaagt
       61 agtcgttctc gcggttggcc gcggtgcgca cgtcaccgta gggctcggag gtggcgggcg
      121 cgtagtcgca gacgtgcgcg cccttgccct cgacgtggag ctggatctct tccggcgtca
      181 gcttgcggtc cgggtgccag gtcaccatcc agttcaccac gtgggtgtcg tccatcggca
      241 cccagatgtg cgactgcacg acccgctcgt cgaggtcggt gggcggcatc gtgtagaacg
      301 ggagcaggaa ctgggtgacg cgccagtacc agcggccgtc cgggttggcc cgacgggcgc
      361 cgatcagcac gccgtaatcg gtgtcgacca cctcgaagcg cggggcccgg tcgccggttg
      421 agacggccgc cccgaccccg aagctcacgc ggtccatgtc ctgggtgacc tcgctgttgc
      481 ggtgatcgat cggcgcgtgg aggaacgaga tgtggctcga gtcgatgcca ccctcgagag
      541 cctggaacca gttgcagtcc tggacccggc gcgacgtgaa cgtgtgtcct tcgggcagca
      601 gcgtccactc gagctccggc aggggcggcg gcgtgctctc cgggcccatg taggcgaaga
      661 ccaccccgcc cgcctccgcg cacgggtagg cgctggtctt caccttcgac gcgaagcccg
      721 actccggcgg ctcgctcggc atctcctcgc actggccgtc gagcccgaac ttccagccgt
      781 ggtaggcgca gcgcatgccc gcgtcttcca cccgcccgta gaagagggac gccgagcggt
      841 gcgggcagaa ctcgccgatc aggccggccc gaccgctctt gccgcggaag gcgatcagcc
      901 gctcgccgag cagctgcacg cgcttcacgc ggccgcctgg cgccaggtcc ttcgactcca
      961 cgacgggaat ccagtagcgg cggaccagat cgcccatcgg cgtccccggt ccggtccggg
     1021 tcaggagctc gttgtcctcg cggctcagca tgcctcgatc ctcctcggcg cgtacggcgt
     1081 gccaacgcgc aaagggtagc acgcgaccgc cgggaggggc cagcgccggc cctcgccttg
     1141 acagcccggt ccggggccac gtacgctccg cccgcgatgc ttgcctctgc cgtcgatctc
     1201 accctcgacg ggccggtggc ccggatcacg ctgaaccggc ccgaggtcct caacgcgggc
     1261 gacccacgct gggtctccga gctcgccgcc gccgtcgcgg cgatcgcggc ccggagcgac
     1321 ctgcgcgtcg cggtgatcgg gggcgcgggc cgcgccttct gcaccggcgt cgatctgaag
     1381 gccctcgcgg ccggcgcgat gacgctgccc cacttcgtgg cgtgggagga cgcgatgacc
     1441 gccatcgagc gcctgcccct gcccttcatc gccgcgatca acggccactg tctgggcggc
     1501 ggccttcagc tggcgctcgt ctgcgactat cgtctcgccg ccgcggaggc caccatcggg
     1561 ctgcccgcgg tgaaggagtg cctcatcccg tcgatggccc tctatcgcct gccccggctg
     1621 atcggagacg cgcgggcccg cgaactgatc ctcaccggcg agccgatcac cgcggcccgc
     1681 gccgagcagt acgggctcgt gcaccgcgtg gtgcccggcg ccgacttcgc gcgggcgctc
     1741 gaggagtccg tcgcgcgctt cctcgccctg cccgcgacca gcgtggcctg gagcaagcgc
     1801 ctcatggcgc gcgccttcga cctcgggttc gaccagttcc gccgcgagat ggaggcgggc
     1861 ttccgcgagt gcctcgactc ggccgagcat cgcgcggcca tggaggacat ccgcgccggc
     1921 atggagaagc ggtcgcgatg accgccgcgc gcccgctcct gcccacctcc gtggtcggca
     1981 gccacgggct gccgggctgg gtctggctcg cgcgcgaggc gatccaggcc gggcggctcg
     2041 gggccaccga tctggccgag ctgatggagg acgcgaccca ggtcgcgctg ctggaccagg
     2101 agcgggccgg cgtcgacgtg ctcagcaccg gcgagatggg gcgcgtgcgc ttcatcatcg
     2161 gcttctacca ccacctgcag ggcatccgcg cgctgcccga gccgcgcctg ctcggccagc
     2221 cgcactggga caccaacacc ccgttccagg tgatcgagaa ggtctcggct ccggccgggc
     2281 tcggcatcgt cgacgagttc aagctcgccc gatcgctcac cacccggccg ctcaaggcca
     2341 ccgtgcccgg gccctacacc ctcatgttcc ccctgcgccc gggcggcccc tatcgcgacc
     2401 gcgacacgct gctggccgat ctggtcgcca tcgtgaacgc ggagtgccgg gcgctggtgg
     2461 acgcgggcgc cgacttcatc cagatcgacg agccccacag tggcatgtac gcggggagca
     2521 cgccccagtt cgcgctcggg gtcaaccgcg cggtggaggg tgtgcgggcc aagatcgcgg
     2581 tgcacgtctg cttcggcaat ctctacgggc ggccgttcca gccggtgcgc agctaccgca
     2641 acgtctaccc cgcgctccac gagctgaccg cctcgcagat cgtcctcgag tacgccaacc
     2701 ggggcatgga cgacctgccc ctgtggacgg agttccccac cgacaaggag ctgggcgcgg
     2761 gcgtcatcga cgtgaaggcc ttcaaggccg agaccgccgc cgaggtggcc gagcgcatcc
     2821 gcgcgctcct ccgccacgtg gcccccgaca agctctggct caaccccgac tgcggcttct
     2881 gggagacccc gcgctgggcc acccgcctca agctcgccgc cctcgtcgaa ggcgcccgcc
     2941 tcgtccgccg cgagctctag tagacccccc gtcagatggc gcggaggagg gtgaggtggt
     3001 cctcgacggt gtcgtgggcg acgatcgcgc ggaggacgac ctcgtcgacg gcgccgcgcc
     3061 aggcggcgag cgcggtcttg aggccctcgg gtgaggtggc tgcgatcgcg gtgtccaccg
     3121 gcttgacgcc catgcgcacg aagtgggcgc cgtacgcggg aatggccgcg tagcgcccgc
     3181 cctcgtcgct gagccggtcg gcggcgcccg ggccgagggc cacccgcacg taggcgtagg
     3241 tccgcggcat cggccgcccg gccgccttgg ccccggcgcg caccagctcg gcggagcggc
     3301 gcgcgtactc cggcgtcagc cagttgaaga gcacgccgtc ggcgacctgc ccggccagct
     3361 cgcacatctt cgggccgagc gcggccacga cgatccgcgt ggtcaagctc ttccggatcg
     3421 cggccacccc gtcgcgcacg cgcgcgagcg cctgcggatt cgggctgccc accccgagca
     3481 ggagccgcgc cggcgcgagc ccgtgctgac ggacgcccgc cacgatggcc tcgggacccc
     3541 gcgtgtgcag cgggatgacc ccgacgccca gatcgatgcg ctgcgtctcc gcgcccccct
     3601 gcgcgagcga gcccaggccg tcgacgggcc cgggatggtt cacccagaac gaggtgaaac
     3661 cgcgcgcctc ggcctcacgc gccgacgcgc ggatgatctc cggcgccacg cccgcaaaga
     3721 ccgcgaagcc cttcgccata tctgccgcca tgaccgcctc ctgttggtgt ggtgggcccg
     3781 atcatacgcc gcgccggcct tgcgctccat cgcaatacgg gatacacccg aacggtcatg
     3841 ccagcccccg cgcgtacgcg catcctgacg tggatgtgcg tgctcatcgc ggtgaaccag
     3901 ctcggcttcg gcagcatcgt gcccgcgctc gccctctacg cgcgctcctt cggcgtcgcg
     3961 cagtccgcca tcggcctcgc catcgcggtc tacgggctcg cgcgattcct ggtcgcggtg
     4021 cccgccggcc gtctcgcgga ctcgctcggg cgccgcacga cgctcgcggt cggcggcctc
     4081 gtgtcgaccc tcggcaacgt gctctgcgcg atcgccccga cctaccccgc gttcgtcggc
     4141 gcgcgcttcg tggccggcgc gggcgccgcc ctcgtgctca ccaccggcct gatcgtgctc
     4201 gcggacatct ccacgccggc caatcgcggc cgcatgatgt cggtctacca gggcgtgttc
     4261 atcttcgcag tcgggatcgg ccccttcccc ggcggaccgc tcgccaccca ctgaggcctc
     4321 gccgcgccgt tcctcgccta cgcggccacg tcgctcatcg cgtccggggt ggcctggttc
     4381 gcgatcccgg agacgcggcc cgcgcggccc gccggcgagg ccgaggcggc ggagatcccg
     4441 ttccgcgcgc agctccgcct cctgaccgtc caccggggct tcctgctcgt gagcctgatc
     4501 gccttcgtga acgcggtggc ccgcaccgga gcgctcttca acatcatccc gatcctggcc
     4561 cgcgaccggc tcgcgctcga gccggaccgc atcgggttcg gcctcgcgct ggcgagcgtg
     4621 gttggcctcg cgctcgctta ccccgcgggc gtgctcgtcg accgctacgg gcgaaagatc
     4681 atcatcgtgc cggccaccgt tctgtccggg atctcgctcc tgctgttcct gatggcgccc
     4741 acctacgctt ggttcctggt cgcctgcgcg gcgtggagcg tggcgatggg gatcagcggc
     4801 gccgcacccg cggcctacgc ggcggacacc gcgccggccg gcatgaacgc gtccgcgctc
     4861 ggcacctacc ggatgctctc ggacc
//
LOCUS       DATMCV010001051         3007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_462011, whole genome shotgun sequence.
ACCESSION   DATMCV010001051 DATMCV010000000
VERSION     DATMCV010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462011"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VNU03_24120"
     CDS             complement(<1..680)
                     /locus_tag="VNU03_24120"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HWP77632.1"
                     /translation="MPNRLRILVMASEVAPFAKTGGLADVAGALPRALAALGHDVRVM
                     MPRYRGAEAHATDSRIVVPTIRVPLGDRAAEGALIETHGPSGVPVYMLEHEHYYNRDS
                     LYGTADGDYWDNCERFVFFCRAALEGLTQLDAARPGVRWQPQLIHANDWQTGLVPIYL
                     RTLYRDHPLLGGLASVFTVHNLAYQGVFWHYDMPMTGLGWDLFTPAGIEFYGKVNFLK
                     GALIFSDLL"
     gene            complement(681..2288)
                     /locus_tag="VNU03_24125"
     CDS             complement(681..2288)
                     /locus_tag="VNU03_24125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HWP77633.1"
                     /translation="MTLLSLFGGLALLLYGMQLIGEGLQRAAGGHLRHLLTRMTRTRL
                     AAVASGALVTAIIQSSSATTLMLIGFVSAGLLTFPQSLGMILGADIGTTVTVQLLAFR
                     VQELSLLLVGAGFALTFFSRRGLLKSLGQVVLGFGFIFLGMKVMNDGLAPLADNELTR
                     QVLVALAGNQFLALLVGAVLSASMASSAATIGLTLSLGQQGLLPLDAAIPVVLGANIG
                     TCATALAASMRSSSDARRVAVAHIAFKVLGVALVFPFIVPLTHLVGLTSADPARQIAN
                     THTFFNVAISALFLPFAPWAARVITALVPEEERGDNPYRTRYLDDRFLEQPALAIGQA
                     TREALRMGDVAQAMLRDAMVVMRTNNQELLEDVERRDDQLDYLEREIKIFISRLGRET
                     MSPDMAQKEIALISFIGNLENIGDIIDKNLMELARKKLYQGRRFSEAGEAELIEFHSM
                     VSKNLERAIAGFAANDRSLAQEVLDQRPVVRQRERELRDSHLARLRRGLAESLETSEI
                     HLDVLTNLKRISSHITALVYPILEEET"
     gene            complement(2290..2922)
                     /locus_tag="VNU03_24130"
     CDS             complement(2290..2922)
                     /locus_tag="VNU03_24130"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWP77634.1"
                     /translation="MRRLLLARHGQSVSNAVRRFQGAQDVALSPLGVRQAEALALAIE
                     RRRVAHVYASPLERARRTAEIAVAGSGVPLTLMDDLRELSLGDWEGCTVDEIRTQPGD
                     PYTRWIRDPVLCLPPGGEPLADVQARVLRSVDRIAAAHGNGDDVLIVAHGGVISALLA
                     HWLGLPLSSIWRLAVANCSLTEIAPPRVVSMNETGHLREVNVVVTEPLSP"
BASE COUNT          461 a         1027 c         1116 g          403 t
ORIGIN      
        1 agcaggtcgg agaagatcag cgcgcccttc aggaagttga ccttgccgta gaactcgatg
       61 cccgcggggg tgaagaggtc ccagccgagc ccggtcatcg gcatgtcgta gtgccagaac
      121 acgccctggt aggccaggtt atgaacggtg aagacgctgg cgaggccgcc caggagcgga
      181 tggtcgcggt agagggtgcg caggtagatc ggcaccagcc cggtctgcca gtcgttggcg
      241 tggatgagct ggggctgcca gcggaccccg ggccgggcgg cgtcgagctg ggtgaggccc
      301 tcgagcgcgg cgcggcagaa gaagacgaac cgctcgcagt tgtcccagta gtccccgtcc
      361 gcggtgccgt agaggctgtc gcggttgtag tagtgctcgt gctcgagcat gtacaccggc
      421 acgcccgagg ggccgtgggt ctcgatgagc gcgccctccg cggcgcggtc gccgagtggc
      481 acccggatgg tcggcaccac gatgcgggag tcggtggcgt gggcctccgc gccgcggtac
      541 ctcggcatca tcacgcgcac gtcgtggccg agggcggcga gcgcgcgcgg cagggccccc
      601 gccacgtccg cgagcccgcc ggtcttggcg aagggcgcga cctcggaggc catgaccagg
      661 atgcggagcc ggttcggcac ctaggtctcc tcctccagga tcgggtacac cagcgcggtg
      721 atgtgggagg agatccgctt gaggttggtg agcacgtcca ggtggatctc ggaggtctcg
      781 agcgactcgg cgaggccccg ccgcagccgg gccaggtgcg agtcgcgcag ctcgcgctcg
      841 cgctgccgca ccaccggccg ctggtcgagc acctcctggg cgaggctccg gtcgttggcc
      901 gcgaagcccg cgatggcccg ctcgaggttc ttcgagacca tgctgtggaa ctcgatcagc
      961 tccgcctccc ccgcctccga gaagcgccgg ccctggtaga gcttcttgcg cgccagctcc
     1021 atgaggttct tgtcgatgat gtcgccgatg ttctcgagat tgccgatgaa ggagatgagg
     1081 gcgatctcct tctgggccat gtccgggctc atcgtctccc gcccgagccg cgagatgaag
     1141 atcttgatct cgcgctcgag gtagtcgagc tgatcgtcgc gacgctccac gtcctcgagc
     1201 agctcctggt tgttggtgcg catcaccacc atcgcgtccc gcagcatcgc ctgggccacg
     1261 tcgcccatgc ggagggcctc gcgggtggcc tgcccgatgg cgagcgcagg ctgctccagg
     1321 aagcggtcgt ccaggtaccg cgtccggtag ggattgtccc cccgctcctc ctccggcacc
     1381 agcgcggtga tcacccgggc cgcccagggg gcgaagggca ggaagagcgc gctgatggcc
     1441 acgttgaaga aggtgtgggt gttggcgatc tgccgggccg ggtccgccga ggtcagcccg
     1501 accaggtggg tcagcgggac gatgaacggg aagacgagcg cgacccccag caccttgaac
     1561 gcgatgtggg ccaccgcgac ccgccgggcg tccgaggacg agcgcatgct cgcggccagc
     1621 gcggtggcgc aggtgccgat gttggccccc agcaccaccg ggatggcggc gtcgagcggc
     1681 agcaacccct gctggccgag cgacagggtg agcccgatgg tcgccgccga ggaggccatg
     1741 ctcgcgctca gcaccgcgcc caccaggagg gcgaggaact ggttgccggc cagggccacc
     1801 agcacctggc gggtcagctc gttgtccgcg agcggggcga gcccgtcgtt catcaccttc
     1861 atgccgagga agatgaagcc gaagcccagc acgacctggc cgaggctctt gagcagcccc
     1921 cgtcgcgaga agaaggtcag cgcgaagccc gcgcccacca gcaggagcga cagctcctgg
     1981 acccggaacg cgagcagctg cacggtgacg gtggtgccga tgtcggcccc caggatcatc
     2041 cccagcgact gggggaaggt caggaggccg gccgacacga agccgatcag catgagggtg
     2101 gtggcggacg aggactggat gatcgcggtc accagggcgc ccgaggccac cgcggcgaga
     2161 cgggtgcgcg tcatgcgggt gagcaggtgg cgcaggtggc cgcccgccgc ccgctgcagc
     2221 ccctcgccga tgagctgcat cccgtagagc agcagggcga gaccgccgaa gagcgacagc
     2281 agcgtcatgt caggggctga gcggctcggt gaccaccacg ttcacctcgc gcagatggcc
     2341 ggtctcgttc atcgacacca cccgcggcgg ggcgatctcg gtgagcgagc agttggccac
     2401 cgcgagccgc cagatcgagg agagcggcag ccccagccag tgcgcgagga gcgcgctgat
     2461 caccccgccg tgcgcgacga tcagcacgtc gtcgccgttg ccgtgcgcgg ccgcgatccg
     2521 gtcgaccgag cggagcaccc gcgcctgcac gtccgcgagg ggctcgccgc ccgggggcag
     2581 gcagaggacc gggtcgcgga tccaccgggt gtagggatcg ccgggctgcg tgcggatctc
     2641 gtccaccgtg cagccctccc agtcgcccag cgacagctcg cgcagatcgt ccatcagcgt
     2701 gagcggcacg ccggatccgg ccaccgcgat ctcggcggtc cgccgcgccc gctcgagcgg
     2761 gctcgcgtag acgtgggcca cccggcggcg ctcgatggcg agcgccaggg cctccgcctg
     2821 gcgcaccccg agcggggaga gggccacgtc ctgggcgccc tggaagcgcc ggaccgcgtt
     2881 ggagaccgac tgcccgtggc gcgcgaggag gagccgcctc accgggccgc ccccaccgcg
     2941 gccgccgccg ccttgaggcg cgcgagggtc tcggcccggc ccaggatgct cacgacctcg
     3001 aagatcg
//
LOCUS       DATMCV010001052         7188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_192047, whole genome shotgun sequence.
ACCESSION   DATMCV010001052 DATMCV010000000
VERSION     DATMCV010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192047"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VNU03_24135"
     CDS             complement(<1..532)
                     /locus_tag="VNU03_24135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77635.1"
                     /translation="MHTPVDYLDPKLPPGLTLAPIRVVDATEESLRDYGHLVSDPRER
                     PIEIVRWPAQGSRPVDADTGDQGGTTEGVFVSEWQGDILYGRNEAVGGHYVLAYGVPP
                     ERARTDHADPPRRMLLWHANYHPDGGQLFFPLDPRPFYASRGRRDPGALRVLPLRRPP
                     RALHPPQHLARGRVRRV"
     gene            670..1644
                     /locus_tag="VNU03_24140"
     CDS             670..1644
                     /locus_tag="VNU03_24140"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP77636.1"
                     /translation="MPFAYFNRLTRRQQAIYLESDGIATVPLPQAARLQPLVTALARA
                     LESGDRAHTESAAQRLVLGLAAALGAPPARVKVLAARPHAGWGELHGLYETTHRAAEP
                     PVITLWMRTARQKRVVAFRTFLRTLLHEVGHHVDYTLLRLGDSFHTQGFYARESHLFH
                     QLVTDGGILMASLEEQMSRMARTGDDFAAAIKGVSDAALTKRPDDKSWSAKETLCHLR
                     DTEESFMTRFQSIMEIDEPRFMAAEPDRWATERQYQRNDAGEALQAFRTRREETLRFL
                     HGLRPEHWDRGGVHSTRGRMTVKDFVALIAWHDDNHLDQLKRALAGKP"
     gene            complement(1641..2543)
                     /locus_tag="VNU03_24145"
     CDS             complement(1641..2543)
                     /locus_tag="VNU03_24145"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018725603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HWP77637.1"
                     /translation="MTDPATRLARALRSLDGLSIGDAFGEAFYSDPTTLAPRIEARIP
                     PDAPWYYTDDTIMAMSVVETLEEHGRIEQDALAARFGRKLVLDPWRGYGRVTHQIVSA
                     IARGGDWRRLAAEAFGGVGSMGNGGAMRAGPIGAYFAGDLATAAAQARLSAEVTHANV
                     EGQAGAMAVAAAAAWVASGAGDAAGLFDAVLDLVPASETRTGIEQAAALGGEMDVLSA
                     MTVVGNGLLSLSQDTVPLALWCVRHHLDDYPAALWAAVGAFGDADTLGAIVGGIVAMR
                     DGGRGVPEEWRRSRESLSIFTRYG"
     gene            2646..3773
                     /locus_tag="VNU03_24150"
     CDS             2646..3773
                     /locus_tag="VNU03_24150"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HWP77638.1"
                     /translation="MARLRTRKWLLAALASLTSLLAVGFYALAQAPDEGKRDRKVAVA
                     GGTFHVLPATLETTQWGWLDPAEPPKLVVNSGDTVAVETMMHAHNKIQPGTTMDEIVA
                     LRKANPGGGPHSITGPIYVNGAEPGDVLEVRILKITPKAFATNFNLPGKEFPTIGALA
                     SEMPDGFVKYFYLDLDKKQAEFKPGVTIDLQPFPGTLAVGIDPKDPSPRKGGSTDPMA
                     PVSTLRPWKNGSNMDINELQAGSTIFFPVFLKGALVWTGDSHCRQGNGEVNLTALECS
                     YREMVMQLIVRKDMKVEFPRIETPTHFIMTGFDEDLNKAMVIAVRETVDFLAAQKMVP
                     LTRYEAYSLTSMVGDCRVSQVVDVRKGVHCMVPKSIFTKKS"
     gene            3773..4705
                     /locus_tag="VNU03_24155"
     CDS             3773..4705
                     /locus_tag="VNU03_24155"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWP77639.1"
                     /translation="MRGALAGALLWLAAAPAWGGGPLPVVTTTTDLKALVEAVGGDRV
                     RATSLAPALHDPHSVEVKPGALAELRGAALLVRIGLDHEPWLARPLRAVGDPRLLPGG
                     AADLDVSRGVALLETETPRVRAERGVHVHGFGNPHYWLDPENARPMTERIQAALGALA
                     PADAAVFAANRARFLQGLDAGLVRWRSALAPYAGTRVVVAHESWPYFARRFGLTVVAA
                     LEPTPGVPPSAAYLAALTARMKAAGVRVIIAEPSTSASLVAQVAARADARAVTLIPSV
                     GGDPEARDYLTLFDVDVARLVAALGAAPAAPVSR"
     gene            4705..6003
                     /locus_tag="VNU03_24160"
     CDS             4705..6003
                     /locus_tag="VNU03_24160"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWP77640.1"
                     /translation="MLDFLWLPFLACLILTGIHVYLGLHVLARGVIFVDLALAQVAAL
                     GISVAFLAGHPIQSDAAYWYALAFTVAGAALFAASRAHRAPLPQEAVIGIVYAVSAAA
                     AVLVVDRAPQGGEHIKTLLVGSILTVSGREVAELALLYGLIGALHAAVRRPLLEISLD
                     PDATRAHGRRLRAWDLLFYVTFGIVVTSSVRLAGVLLVFSYLIVPAAVATLLAASVPA
                     RLLIGWGVGALVSAAGLWASFAWDLPTGAAVVTSFGVLMALVALGLGAHRLAQGVRAQ
                     GVGALRGVGTLLALAIAGAGLLLLVFPGMDHPWLDWIEAAAPSVRLAFLTPGERAAHR
                     DSREAMDRGVAELARLRALQQDVQWGARAMPAEQQERLRQFLAGRAEIVAGDRIVLAT
                     LRSHARARQRVWLGLPLLLAGAGVAVWLARPRATSRDAGG"
     gene            complement(5981..6973)
                     /locus_tag="VNU03_24165"
     CDS             complement(5981..6973)
                     /locus_tag="VNU03_24165"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77641.1"
                     /translation="MTRLRVSLRVPVGRPLPELADVVARCEAAGLDGVGIHDHPSSGR
                     DAYVALALAARATRRLVLFPATSSPIVRHPLLLASLAHSLEELAPGRARLTMAPGFIS
                     TRSIGRPRGTVTIMREAVRDVRRLLAGEAVGFGPTETRLRNRSPVPTPVYLLAAGPRM
                     TELAGEVADGAFLMVGLDAAAIRAARRHLAEGARRAGRSLDGFPVVFVVTLGLGADAA
                     VGARWVRSWFAPGQPFLAYPSAANLRWLAEAGFPLPEPLDPAAIAEDQARRIADAFGL
                     FGPPERCAERLLQAREEAGVDEVFLFPAHDLAGGYEMPEAEMEAFERIIRPRLG"
     gene            complement(6970..>7188)
                     /locus_tag="VNU03_24170"
     CDS             complement(6970..>7188)
                     /locus_tag="VNU03_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWP77642.1"
                     /translation="LEPPRLVELTWVTGAGGTKGAETVVTVELEPQGRGTHVRLTHAG
                     FPDDESRRGHVRAWPLVLEQLEQRLAQR"
BASE COUNT          936 a         2745 c         2540 g          967 t
ORIGIN      
        1 gcacgcgccg aacacgccct cgtgccagat gttggggtgg atgtagagcc cgtggaggcc
       61 gtcgaagcgg aagcacacga agcgctccgg ggtcacgtcg tccccgggaa gcgtagaacg
      121 gccgcgggtc cagcgggaag aacagctgcc cgccgtcggg gtggtagttc gcgtgccaga
      181 gcagcatccg gcgcggtgga tccgcgtggt cggtccgcgc gcgctccggc ggtaccccgt
      241 aggccagcac gtagtgcccg ccgaccgcct cgttgcgccc gtacaggatg tcgccctgcc
      301 actcgctgac gaacacgccc tcggtggtcc cgccctgatc cccggtatcc gcgtcgaccg
      361 ggcggctgcc ctgtgcgggc catcggacga tctcgatcgg gcgctcgcgc ggatcggaga
      421 cgagatgccc gtagtcccgc aggctctcct cggtggcgtc caccacccgg atcggcgcca
      481 gggtgagccc gggcggcagc ttcgggtcga ggtaatcgac cggggtgtgc acgtgggctc
      541 tcctgtccgg gtgtgagggc agagtcgggc gcgctcaccg tagaacaccc gcccgagcgg
      601 cgtcaacgcc ttcccgcccg cgccgtcaag ttgcccccgc cctccctcgg cggtagcatc
      661 cttgagcgga tgcccttcgc ctacttcaac cggctcacgc ggcggcagca ggcgatctat
      721 ctcgagagcg acggcatcgc cacggtgccc ctgccgcagg ccgcgcggct ccagcctctg
      781 gtcaccgcgc tggcccgcgc gctcgagagc ggcgaccgcg cccacaccga atcggccgcc
      841 cagcgcctgg tcctcgggct ggccgccgcg ctgggcgcgc cgcccgcacg ggtgaaggtg
      901 ctggccgcgc ggccgcacgc gggctggggc gagctgcacg gcctctacga gaccacgcac
      961 cgggccgccg agccgccggt gatcacgctc tggatgcgca ccgcgcggca gaagcgcgtg
     1021 gtggccttcc gcacctttct gcgcacgttg ctccacgagg tcggccacca cgtggactat
     1081 actctgctgc ggctgggcga ctccttccac acgcaggggt tctacgcgcg ggagtcgcac
     1141 ctgttccatc aactggtcac cgacgggggg attctcatgg cgagtctgga agagcagatg
     1201 tcccgcatgg cgcgcaccgg cgacgacttc gcggccgcca tcaagggcgt ctcggacgcg
     1261 gcgctcacca agcggccgga cgacaagagc tggtcggcca aggagacgct gtgtcacctg
     1321 cgggacaccg aagaatcctt catgactcgc ttccagtcca tcatggagat cgacgagccc
     1381 cggttcatgg ccgcggagcc cgaccgctgg gcgaccgagc gtcagtacca gcgcaacgac
     1441 gcaggtgagg cgctccaggc cttccggacg cgccgcgagg agacgctgcg gttcctgcac
     1501 ggcctgcggc ccgagcactg ggaccgcggt ggggtgcact ccacgcgcgg gcgcatgacc
     1561 gtgaaggact tcgtcgccct catcgcctgg cacgacgaca accacctgga ccagctcaag
     1621 cgcgcgctcg cgggcaagcc ctagccgtac cgcgtgaaga tcgacagcga ctcgcgggag
     1681 cgccgccact cctcgggcac gccgcgcccg ccgtcccgca tggccacgat gccgcccacg
     1741 atcgcgccca gggtgtcggc gtcgccgaag gcgccgaccg cggcccacag ggcggccgga
     1801 tagtcgtcca ggtggtgccg cacgcaccac agggcgagcg gcacggtgtc ctgggagaga
     1861 gacagaagcc cgttccccac caccgtcatc gcgctcagga cgtccatctc gccgccgagc
     1921 gcggccgcct gctcgatgcc ggtgcgcgtc tcgctggccg gcaccaggtc gaggacggcg
     1981 tcgaagaggc ccgcggcgtc gccggccccg ctcgcgaccc aggccgccgc ggccgccacc
     2041 gccatcgcgc ccgcctgtcc ctcgacgttg gcgtgggtca cctccgcgga caggcgcgcc
     2101 tgcgccgcgg ccgtcgcgag gtcgcccgcg aagtaggcgc cgatcggacc cgcccgcatc
     2161 gcgccgccgt tgcccatgga gccgaccccg ccgaacgcct ccgccgcgag ccgccgccag
     2221 tcgccgccgc gcgcgatcgc gctcacgatc tggtgggtga cccgtccgta gccgcgccac
     2281 gggtcgagca cgagcttgcg gccgaagcgc gcggcgagcg cgtcctgctc gatgcggccg
     2341 tgctcctcca gggtctccac caccgacatc gccatgatgg tgtcgtcggt gtagtaccac
     2401 ggcgcgtcgg gtgggatgcg ggcctcgatc cgcggggcca gcgtggtcgg atcgctgtag
     2461 aacgcctccc cgaaggcatc gccgatcgac aggccgtcca gcgagcgcag ggcccgcgcg
     2521 agacgcgtgg cggggtcagt cacgatgaaa gcctccccgg cgatcatacc ctgctagaat
     2581 gccgcccgcg gtcggtccga gggagtaccg gcacgtgacc ctacaccccc acaggaggac
     2641 acgtgatggc caggctcagg acccgtaagt ggttgctcgc tgctctcgcc tcgctgacca
     2701 gcctgctcgc ggtcggcttc tacgcgctgg cccaggcccc cgacgagggc aagcgcgacc
     2761 ggaaggtcgc ggtcgccggc ggcaccttcc acgtgctgcc cgccacgctc gagaccaccc
     2821 agtggggctg gctcgatccg gccgagccgc ccaagctggt ggtcaactcc ggcgacacgg
     2881 tggcggtcga gacgatgatg cacgcgcaca acaagatcca gcccggcacc accatggacg
     2941 agatcgtggc gctgcggaag gccaacccgg gcggcggccc gcactcgatc accgggccca
     3001 tctacgtgaa cggggcggag ccgggcgacg tgctcgaagt ccgcattctc aagatcacgc
     3061 ccaaggcctt cgccaccaac ttcaacctgc ccggcaagga gtttccgacc atcggcgcgc
     3121 tcgcgtccga gatgccggac ggcttcgtga agtacttcta cctcgacctc gacaagaagc
     3181 aggccgagtt caagcccggc gtcaccatcg acctgcagcc gttcccgggc accctcgcgg
     3241 tgggcatcga ccccaaggat ccctcgccgc gcaagggcgg ctccaccgat ccgatggcgc
     3301 cggtctccac gctccgcccg tggaagaacg gctccaacat ggacatcaac gagctgcagg
     3361 cgggctcgac catcttcttc ccggtcttcc tcaagggcgc gctggtgtgg accggcgact
     3421 cccattgccg ccagggcaac ggcgaggtca acctcaccgc gctcgagtgc tcctaccgcg
     3481 agatggtcat gcagctcatc gtgcgcaagg acatgaaggt ggagttcccg cgcatcgaga
     3541 ccccgaccca cttcatcatg accggcttcg acgaggacct gaacaaggcg atggtcatcg
     3601 cggtgcgcga gacggtcgac ttcctcgccg cccagaagat ggtgccgctc acgcgctacg
     3661 aggcctactc gctgacctcc atggtcggcg actgccgggt gagccaggtg gtggacgtcc
     3721 gcaagggcgt gcactgcatg gtgcccaaga gcatcttcac caagaagtcg tagtgcgcgg
     3781 cgcgctcgcc ggcgcgctgc tctggctggc cgccgctccc gcgtggggcg gcggccccct
     3841 gcccgtcgtc accaccacca ccgatctgaa ggcgctcgtg gaagcggtcg gcggcgaccg
     3901 cgtgcgcgcg accagcctgg cccccgcgct gcacgacccg cacagcgtgg aggtcaagcc
     3961 cggcgcgctc gccgagctgc gcggggccgc gctgctggtg cgcatcggcc tcgaccacga
     4021 gccgtggctg gcccggcccc tgcgcgcggt gggcgacccg cggctcctgc ccggcggggc
     4081 ggccgacctc gacgtctcgc gcggcgtcgc cctgctggag accgagaccc cccgcgtgcg
     4141 cgccgagcgc ggcgtgcacg tgcacggatt cggcaacccg cactactggc tcgaccccga
     4201 gaacgcgcgc cccatgaccg agcgcatcca ggccgcgctc ggcgcgctgg cccccgcgga
     4261 cgccgccgtc ttcgcggcca accgtgcccg cttcctccag ggcctcgacg cgggcctcgt
     4321 ccgctggcgg agcgcgctcg ccccctacgc gggcacccga gtggtggtgg cgcacgagag
     4381 ctggccctac ttcgcgcggc gcttcggcct cacggtggtc gccgcgctcg agccgacgcc
     4441 gggtgtgccg ccctccgccg cctacctggc cgccctcacc gcccgcatga aggcggccgg
     4501 ggtgcgggtc atcatcgccg agccgtccac cagcgcgtcg ctggtggccc aggtggccgc
     4561 gcgcgccgac gcccgcgcgg tgacgctcat cccctcggtc ggcggcgacc ccgaggcccg
     4621 cgactacctc acgctcttcg acgtcgacgt cgcgcgcctg gtggccgcgc tcggcgccgc
     4681 gcccgccgcg ccggtcagcc gctgatgctg gacttcctct ggctgccctt cctggcctgc
     4741 ctgatcctca ccggcatcca cgtctacctg ggcctgcacg tcctggcgcg cggggtcatc
     4801 ttcgtcgacc tggccctggc ccaggtggcc gcgctcggca tcagcgtggc cttcctggcc
     4861 gggcatccca tccagagcga cgcggcctac tggtacgcgc tggccttcac ggtggccggc
     4921 gccgcgctct tcgcggccag ccgggcgcat cgcgcgccgc tgccgcagga ggcggtgatc
     4981 ggcatcgtct acgcggtctc cgcggcggcc gcggtgctgg tggtggaccg cgccccgcag
     5041 ggcggcgagc acatcaagac cctgctggtg ggcagcatcc tcaccgtgag cggccgcgag
     5101 gtggcggagc tcgcgctgct ctacgggctg atcggcgcgc tccacgccgc ggtgcgccgg
     5161 ccgctcctcg agatctcgct cgaccccgac gccacgcgcg cccacggccg gcgcctgcgc
     5221 gcgtgggatc tcctcttcta cgtcacgttc ggcatcgtgg tgaccagctc ggttcgcctg
     5281 gccggtgtgc tgctcgtctt ctcgtacctg atcgtgcccg ccgcggtggc gacgctactc
     5341 gccgcctcgg tgcccgcgcg cctgctgatc gggtggggcg tgggcgcgct cgtgagcgcg
     5401 gccggcctct gggcctcgtt cgcgtgggac ctgccgaccg gcgcggcggt ggtcacctcg
     5461 ttcggcgtgc tgatggcgct ggtggcgctc ggcctcggcg cgcaccggct cgcgcagggg
     5521 gtgcgcgcgc agggcgtggg ggcgctccgc ggcgtgggca cgctgctcgc actggcgatc
     5581 gccggcgcgg ggctgctgct cctggtcttc cccggcatgg accatccgtg gctcgactgg
     5641 atcgaggccg cggcgccgtc ggtgcgcctg gccttcctga cgccgggtga gcgcgccgcc
     5701 catcgcgaca gccgcgaggc gatggaccgc ggcgttgccg agctggcgcg gctgcgcgcg
     5761 ctccagcagg acgtgcagtg gggggctcgt gcgatgccgg ccgagcagca ggagcgcctg
     5821 cgccagttcc tggccggccg cgccgagatc gtggcgggcg atcgcatcgt gctggccacg
     5881 ctccggtccc acgcgcgcgc ccgccagcgc gtctggctcg gactgccgct gctgctcgcg
     5941 ggtgcgggcg tcgccgtgtg gctcgcgcgc ccgcgcgcga ctagccgaga cgcgggcgga
     6001 tgatgcgctc gaaggcctcc atctccgcct ccggcatctc gtagcctccg gccaggtcgt
     6061 gggccgggaa gaggaacacc tcatcgaccc ccgcttcctc ccgcgcctgc agcagccgct
     6121 ccgcgcatcg ctccggcggc ccgaagagcc cgaacgcgtc cgcgatccgg cgcgcctggt
     6181 cctcggcgat cgcggcggga tcgagcggct ccggcagcgg gaatcccgcc tccgcgagcc
     6241 agcgcaggtt cgccgcgctc gggtacgcga ggaacggctg ccccggcgcg aaccagctcc
     6301 gcacccaccg cgcgcccacc gccgcgtcgg cgcccaggcc gagggtcacc acgaagacca
     6361 ccgggaagcc gtccagcgac cggccggcgc ggcgcgcgcc ctccgccagg tggcggcgcg
     6421 cggcgcggat ggcggccgcg tcgaggccga ccatcaggaa cgcgccgtcg gcgacctcgc
     6481 cggccagctc ggtcatgcgc ggaccggccg cgagcaggta caccggggtg gggaccgggc
     6541 tgcggttccg cagccgcgtc tcggtcggcc cgaagccgac cgcctcgccg gcgagcagcc
     6601 ggcgcacgtc gcggaccgcc tcgcgcatga tcgtcaccgt gccgcgcggc ctcccgatgc
     6661 tacgcgtcga gatgaacccc ggcgccatgg tgagccgcgc gcggcccggc gccagctcct
     6721 ccaggctgtg cgcgagcgag gccagcagca gcggatgccg cacgatcggg ctcgacgtgg
     6781 cgggaaagag aacgagacgc cgggtcgcgc gggcggccag ggccagggcg acgtacgcgt
     6841 cgcgcccgct cgacgggtgg tcgtggatgc cgacgccgtc gaggccggcc gcctcgcagc
     6901 gcgcgacgac atcggccagc tcgggcagcg gccggcccac cggcactcgc aggctgaccc
     6961 ggaggcgggt catcgctggg cgaggcgctg ctccagctgc tccagcacca ggggccacgc
     7021 gcggacgtgc ccgcgtcgcg actcgtcgtc cggaaacccg gcgtgggtga gccgcacgtg
     7081 ggtgcccctc ccctggggct cgagctcgac ggtgaccacg gtctcggcgc ccttcgtccc
     7141 cccggccccg gtcacccacg tgagctcgac cagccggggt ggctcgag
//
LOCUS       DATMCV010001053         4779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_856633, whole genome shotgun sequence.
ACCESSION   DATMCV010001053 DATMCV010000000
VERSION     DATMCV010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856633"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VNU03_24175"
     CDS             complement(<1..749)
                     /locus_tag="VNU03_24175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77643.1"
                     /translation="MPVLLAALGALLMSLDSAINIALPAIAEAFGVEPAAIRWVIICY
                     VLTYALTAFVAGVLADRFGPGPVFTVGLWLSGLVFIGYAFAPAYGTVLLLRMAQGLGG
                     GLVYGTAPALVTLSLPRERHGRGLGLMSLGIGVGLGVGPLLGGGLLAGFGWAATFVFR
                     APLFLALAVLTQTQGRRLGGAAPVRRRFALADLLRLPVLRAALLAFLSNHAQFAVWLL
                     VPFFLVGTLGLSPTVGGLVFALTPLANALVAP"
     gene            827..1237
                     /locus_tag="VNU03_24180"
     CDS             827..1237
                     /locus_tag="VNU03_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWP77644.1"
                     /translation="MPLVPGQTAEVRRVVTPDLTADVLGNAGVTVFATPFVVTLIENA
                     AHAVMVPHLPPGGATVGTAVDMRHLAATPVGMTVRAKAVLRETDGRRCLFDVEAWDEV
                     EKIAEGRHERYVVSNLARFLERTTAKGRGPARAS"
     gene            complement(1234..1803)
                     /locus_tag="VNU03_24185"
     CDS             complement(1234..1803)
                     /locus_tag="VNU03_24185"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWP77645.1"
                     /translation="MADAAHPPHRHLSPETIRFCPLCGAGLVPKPIGADKRPEMVCSG
                     CEFIFYVNQKVVAGTIPWQDGRLLLTRRAIEPAIGKWTFPGGYVDWGETVDAAALRET
                     WEETGLRVELDGLIGVYSYATAPVVIVVYRARVLGGTLTLCHENDRLEWVTPAEVRWE
                     DLAFPSTVAAMRDFLARPSSGDHAPGNAS"
     gene            1893..3329
                     /locus_tag="VNU03_24190"
     CDS             1893..3329
                     /locus_tag="VNU03_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWP77646.1"
                     /translation="MKWVTPRSGFDEVFDARGQARPHYAPLISILESFTKEDVERRER
                     LQQLTLRNQGVTFTVYGEKDGLERIFPFDFVPRIIPAAEWKGIQDGLVQRITTLNLFL
                     ADIYQDQKCLKDGILPAELILSRKEYKRALLGLVPPRKIYTHVVGTDIIRDDRGEYLV
                     LEDNCRVPSGVSYVLENRNLLSRVFPEFLGSYPVRPIKDYPTLLLETLRFVAPRRGQN
                     PVVVILSPGIHNSAYFEHSFLAREMGVPLVEGRDLLVEGNHLFMRRTHGKRQVDVIYR
                     RIDDDFLDPLAFRRDSMLGVPGLLNVYREGNVALANAPGAGVADDKSVYAYMPKLIKY
                     YLGEDPLLPQVPTYLGHKKDDFKFIGEHLDQLVVKTTGDAGGYGMLMGPFAAAKERES
                     YLAAMKKNPGNYIAQPLVELSTHPTHIGGKFEPRRVDLRPFILYGDRIRVLPGGLTRV
                     ALRAGSYVVNSSQGGGSKDTWVLAPREA"
     gene            3326..4285
                     /locus_tag="VNU03_24195"
     CDS             3326..4285
                     /locus_tag="VNU03_24195"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWP77647.1"
                     /translation="MILSRVADGLYWMGRYLERAENTTRLLLVTEELSTEVLGLDEDL
                     ARGEWRDLRAIFPGFPEGEPPFRHLAGLASASLLGLSIDPENRYSVFHSLKKARDNAR
                     SVREALSLEVFINLNETYRELEGQTRRHLADPATFRSEMQSTHRGLMSTVGAIEHTLT
                     RDAGWLFLKLGESLERVFRTVVILRTKLPALVSDEPKVDLPLFYSQWRSLLRGLSCLE
                     NYRKVFGARLEPIDVLQFLLFDAQTPRSVRYGASAVKEHLDRISSASDVSQPARIVGK
                     LAAELSYQGHDLIRDGQILSFLDHVLTELGRAHEALSAVYFGS"
     gene            4293..>4779
                     /locus_tag="VNU03_24200"
     CDS             4293..>4779
                     /locus_tag="VNU03_24200"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HWP77648.1"
                     /translation="MFLEIEHTLSLAYDAYIRESFLELRMQPKTTADQTLQSFVLAVG
                     PPTKIARYRDWNGNAVHHFTIVHFHERIEVVARSAVSTHPVAPGLDTVTDARPFPEPS
                     YPLLDFLAFDGPVRPGAALRKFSQGVEAAPTAPIGEVVRACGRHIQEHFTYRKNVTRY
                     DS"
BASE COUNT          776 a         1752 c         1614 g          637 t
ORIGIN      
        1 ggagccacca gcgcattggc gagcggggtg agggcgaaga ccagcccgcc cacggtggga
       61 gagagcccga gcgtgccgac gaggaagaac ggcaccagca gccacaccgc gaactgcgcg
      121 tggttcgaca ggaacgcgag cagcgcggcc ctgaggaccg gcagccggag caggtccgcg
      181 agcgcgaagc gacgcctcac cggcgcggcg ccgcccagcc gacggccctg cgtctgggtc
      241 aacacggcca gggccaggaa gagcggggcg cggaagacga aggtggccgc ccagccgaag
      301 ccggccagca gcccgccgcc gagcaggggc ccgaccccga gccccacccc gatccccagg
      361 ctcatgaggc cgagcccacg gccatggcgc tcgcgcggca gcgagagcgt gaccagcgcg
      421 ggcgcggtgc cgtagacgag cccgcccccc agcccctgcg ccatccgtag gagcaggacc
      481 gtcccgtagg cgggcgcgaa cgcgtacccg atgaacacga ggccggagag ccagagtccc
      541 acggtgaaca ccggacccgg tccgaagcga tcggcgagca cgccggccac gaacgcggtg
      601 agcgcgtagg tcagcacata gcagatgatc acccaccgga tcgcggccgg ctcgacgccg
      661 aacgcctccg cgatcgcggg cagcgcgatg ttgatcgcgg agtcgagcga catgagcagc
      721 gctcccagcg ccgcgaggag cacgggcatt cggcaagtat acgtgccgcc gagccgcgcg
      781 gcggcgttga cgtggcgggg cgatcggggt agaaggagaa gccgccatgc cgctcgtccc
      841 cggacagacc gccgaagtgc ggcgcgtggt cacgcccgac ctcaccgccg acgtcctggg
      901 caacgcggga gtcactgtct tcgcgactcc gttcgtggtg accctgatcg agaacgcggc
      961 ccacgcggtg atggtccccc acctgccgcc gggaggggcc accgtcggga ctgcggtgga
     1021 catgcggcat ttggccgcca cgccggtcgg gatgacggtg cgggccaagg cggtgctgcg
     1081 cgagaccgac gggcgccgct gcctcttcga cgtggaggcc tgggacgagg tggagaagat
     1141 cgcggagggg cgacacgagc gctacgtggt gtcgaatctc gcgcggttcc tcgagcgcac
     1201 caccgccaag ggccggggcc cggcccgcgc gagctaggaa gcgttgcccg gcgcgtgatc
     1261 cccggagctc ggccgcgcga ggaaatcgcg catcgccgcc accgtggagg gaaaggccag
     1321 atcctcccac cggacctccg cgggggtgac ccactcgagc cggtcgttct cgtggcagag
     1381 cgtgagcgtg ccacccagca cccgcgcccg gtagacgacg atgaccaccg gggcggtagc
     1441 gtaggagtag accccgatca gcccgtccag ctccacgcgg aggccggtct cctcccaggt
     1501 ctcgcgcagc gcggccgcgt ccaccgtctc gccccagtcc acgtagccgc cggggaaggt
     1561 ccacttgccg atggcaggct cgatggcccg gcgcgtgagc aggaggcggc cgtcctgcca
     1621 cgggatcgtg ccggcgacca ccttctggtt cacgtagaag atgaactcgc agccggagca
     1681 gaccatctcc gggcgcttgt cggcaccgat cggcttgggc acgagcccgg ccccgcagag
     1741 cgggcagaag cggatcgtct ccggcgagag gtggcgatgc ggcgggtgcg cggcgtcggc
     1801 catggtgccg gtcagtctag cacgcgctcg cgcggcgtct tgacacccgg gggcgcggca
     1861 cgagaggctc gagggcccaa ggagacgcgc gcatgaaatg ggtcacgccc cgcagcggct
     1921 tcgacgaggt cttcgacgcg cggggacagg cgcggcccca ctacgcgccc ctgatctcca
     1981 tcctggagtc gttcaccaag gaggacgtgg agcggcggga gcggctgcag cagctcaccc
     2041 tgcgcaacca gggcgtcacc ttcacggtgt acggcgagaa ggacgggctc gagcgcatct
     2101 tccccttcga cttcgtcccg cgcatcattc ccgccgcgga gtggaagggg atccaggacg
     2161 ggctggtcca gcgcatcacc acgctcaacc tcttcctggc cgacatctac caggaccaga
     2221 agtgcctgaa ggacggcatc ctccccgccg agctgatcct ctcgcggaag gagtacaagc
     2281 gggcgctgct cgggctggtc ccgccccgca agatctacac ccacgtcgtg ggcaccgaca
     2341 tcatccggga cgatcgcggc gagtacctcg tcctggagga caactgccgg gtgccgagcg
     2401 gcgtctccta cgtgctggag aaccgcaacc tcctgagccg cgtcttcccc gagttcctcg
     2461 gctcctaccc ggtccgcccc atcaaagact acccgacgct gctgctcgag accctgcgct
     2521 tcgtggcccc ccgccggggc cagaacccgg tggtggtgat cctgagcccg ggcatccaca
     2581 actcggccta cttcgagcac tccttcctcg cccgcgagat gggggtgccg ctggtggagg
     2641 gccgtgatct cctggtcgag ggcaaccacc tcttcatgcg ccgcacccac ggcaagcgcc
     2701 aggtggacgt catctatcga aggatcgacg acgacttcct cgatcccctc gccttccggc
     2761 gcgacagcat gctgggcgtg cccggactgc tcaacgtcta ccgcgagggc aacgtcgcgc
     2821 tcgccaacgc gccgggcgcg ggggtcgccg acgacaagtc ggtctacgcc tacatgccga
     2881 agctcatcaa gtactacctg ggcgaggatc cgctcctgcc gcaggtgccc acctacctcg
     2941 ggcacaagaa ggacgacttc aagttcatcg gcgagcacct ggaccagctc gtggtgaaga
     3001 ccaccggcga cgcgggcgga tacggcatgc tgatggggcc cttcgcggcg gccaaggagc
     3061 gcgagagcta cctggccgcg atgaagaaga acccgggcaa ctacatcgcc cagcccctgg
     3121 tggagctgtc cacgcacccg acccacatcg gcggcaagtt cgagccgcgg cgggtggacc
     3181 tccgcccgtt catcctctac ggcgaccgga tccgggtgct gcccggcggg ctcacccggg
     3241 tggccctgcg ggccggctcc tacgtggtca actcctccca gggcggcggc agcaaggaca
     3301 cctgggtcct ggcgccccgc gaggcatgat cctctcccgc gtcgcggacg ggctctactg
     3361 gatggggcgg tacctcgagc gcgcggagaa caccacgcgg ctcctgctgg tgaccgagga
     3421 gctctccacc gaggtgctcg gcctcgacga ggacctggcc cgcggcgaat ggcgggacct
     3481 gcgcgccatc ttccccggct tcccggaggg cgagcctccc ttccgccacc tggccggcct
     3541 cgcctccgcg tcgctcctgg gcctctccat cgatccggag aaccgctact cggtcttcca
     3601 ctcgctcaag aaggcgcgcg acaacgcccg ctcggtgcgc gaggccctgt ccctcgaggt
     3661 gttcatcaac ctcaacgaga cctaccggga gctggagggc cagacccggc gccacctcgc
     3721 cgaccccgcc accttccgca gcgagatgca gtccacccac cgcgggctca tgagcacggt
     3781 gggggccatc gagcacacct tgacccgcga cgcgggctgg ctcttcctca agctgggcga
     3841 gtcgctcgag cgggtcttcc gcaccgtggt gatcctccgc accaagctcc ccgcgctggt
     3901 ctcggacgag ccgaaggtcg acctgcccct cttctacagc cagtggcgca gcctgctccg
     3961 cggcctctcc tgcctcgaga actaccgcaa ggtcttcggg gcccggctcg agccgatcga
     4021 cgtgctgcag ttcctgctct tcgacgccca gaccccgcgc tcggtgcgct acggggcgag
     4081 cgcggtgaag gagcacctcg accgcatctc gagcgcgagc gacgtgagcc agcccgcgcg
     4141 gatcgtgggc aagctggccg ccgagctctc ctaccagggc cacgacctga tccgcgacgg
     4201 gcagatcctc tcgttcctcg accacgtgct gaccgagctg gggcgcgcgc acgaggcgct
     4261 ctccgcggtg tacttcggga gctgagccga ccgtgttcct cgagatcgag cacaccctgt
     4321 cgctcgccta cgacgcctac atccgcgagt cgttcctcga gctgcggatg cagcccaaga
     4381 ccaccgcgga ccagaccctc cagtctttcg tgctcgcggt ggggccgccc accaagatcg
     4441 ctcgctaccg cgactggaac ggcaacgcgg tgcaccactt cacgatcgtc cacttccacg
     4501 agcgcatcga ggtggtggcc cgctccgcgg tgagcacgca cccggtcgcc cccgggctcg
     4561 acacggtgac cgacgcccgc cccttcccgg agccgtccta cccgctgctc gacttcctcg
     4621 ccttcgacgg gccggtgcgc ccgggcgcgg cgctgcgcaa gttctcccag ggagtggagg
     4681 cggcgccgac cgccccgatc ggcgaggtgg tgcgggcctg cggccggcac atccaggaac
     4741 acttcaccta ccgcaagaac gtcacccgct acgactcca
//
LOCUS       DATMCV010001054         3951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_55201, whole genome shotgun sequence.
ACCESSION   DATMCV010001054 DATMCV010000000
VERSION     DATMCV010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55201"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1203)
                     /locus_tag="VNU03_24205"
     CDS             complement(55..1203)
                     /locus_tag="VNU03_24205"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWP77649.1"
                     /translation="MRVSRVTPFLADLGGGKNLCFVKVETDGGPHGWGECYTQADRDT
                     SVVALVEAIGRYLVGRDASHITHFVHWAYHDWAAKRGAMDFWSAVSGLEQALWDIAGK
                     RHGAPVHALLGGPCRERVRVYANGWYGGSKTPADYAARARETVARGFTALKFDPLPGP
                     WRTHVPREVEEKAVATVAAVREAVGPAIDLLIEVHRRLAPMHAVRVARELERFRPFWY
                     EEPVSSTNLEALAECRRQIAIPIVTGEELYTRAEFRRCFELRAADIVNPDVCNCGGIQ
                     ELRAIAQMAEPSLVTVSPHNYNSTTVGLAATVQVSAAIPNFLITEYFVNFEARGREIA
                     TPALEVRDGFITVPTRPGLGVDLDEAALRRYPARPLARSMPTPGQEEP"
     gene            1322..2527
                     /locus_tag="VNU03_24210"
     CDS             1322..2527
                     /locus_tag="VNU03_24210"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77650.1"
                     /translation="MTTLFVAQVCGSTGHSISLAVGSIMAASITSTNTWSGVPIAVGA
                     LGTALASWPLARLMDRAGRRPGLALGYGLAVAGAVLGLLGVAVRSFPLMLAGMALFGI
                     ASTSNLLARYAAADVSRPEQRGRAMGIIVGGSALGSIIGPNLMAPALSLGARLDIPDT
                     ASPFLVSVAGYGVAAVLTFLFLRPDPLALARRDAAEANAGRPAGLARSLGAILADVRV
                     QIALATLSISQFVMISTTSTSPLYLHDQGHTVGTIGVAVSLHLGGMYVSSPLSGWLSD
                     RFGRMPVIASGALMLIFAVAIAGLAPGQAGATIILGLFLNGIGWNLAFVSGSALLTDA
                     LSPVERASVQGLADLLMGLMGAIGSAAGGMILGLWGFAILNAVGALLVLGPLGVALLL
                     RPAPAPARG"
     gene            2524..3729
                     /locus_tag="VNU03_24215"
     CDS             2524..3729
                     /locus_tag="VNU03_24215"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77651.1"
                     /translation="MSLAALLRLRDLRCVAAGTVFNSAGNMGELVVLGWLALELTNSP
                     FLVGAALGCRMLPLAIVGVPAGVLADRVARPRLLLITGAGQALTATAMGALTLLGVVT
                     LGQLLLLTVAAGALRGVEHAARQSYAHDVVGPNALVDGLAILGLAMRGGWLVGSLGVG
                     ALIAHRGSGEAYLAVAVAYLLGGLALLPARALAPVAVGAPDSLWRGVTGFATAVREDR
                     MLLVLMLLTAGAEVLGFAHQALLPSLSRDVLHTGPEGLGAMSAARAVGGILGLAVSMR
                     GLLGGGGMVFVGVLVAFGASLVALGGAPYVVGFTGVMVVVLAANAAGALADLMAQSLL
                     QLSVPHHLRGRAGGAWVVAIGFAPLGQLQIGALASLLGVSAALGVSGVALITLASATI
                     FFYPRLRAL"
BASE COUNT          439 a         1462 c         1447 g          603 t
ORIGIN      
        1 cgcccgcggc gaggcccgcc cgtagaaagc tccgacgatc gaggtgaggc gcgctcatgg
       61 ctcctcctgg cccggggtgg gcatcgagcg ggcgagcggc cgcgccgggt accgtcggag
      121 cgccgcctcg tccaggtcca cgccgaggcc gggccgcgtg ggcaccgtga tgaagccgtc
      181 gcgcacctcg agcgcggggg tggcgatctc gcggccccgg gcctcgaagt tcacgaagta
      241 ctcggtgatg aggaagttgg ggatggcggc cgacacctgc accgtggcgg ccagccccac
      301 cgtcgtcgag ttgtagttgt ggggcgagac ggtgaccagc gagggctcgg ccatctgggc
      361 gatcgcccgc agctcctgga tgccgccaca gttgcagacg tccggattca cgatgtcggc
      421 cgcgcgcagc tcgaagcagc gccggaactc ggcgcgggtg tagagctcct cgccggtcac
      481 gatcgggatc gcgatctgcc gccggcactc ggcgagcgcc tcgagattgg tggaggacac
      541 cggctcctcg taccagaacg gccggaagcg ttccagctcg cgggcgacgc ggaccgcgtg
      601 catcggggcg aggcggcggt gcacctcgat cagcagatcg atcgcggggc cgaccgcctc
      661 ccgcaccgcg gccaccgtcg cgaccgcctt ctcctcgacc tcgcgaggca cgtgcgtgcg
      721 ccacggcccg gggagcgggt cgaacttgag cgcggtgaac ccgcgggcca ccgtctcccg
      781 cgcccgcgcc gcgtagtccg ccggcgtctt cgacccgccg taccagccgt tggcgtagac
      841 ccgcacccgc tcccggcacg gcccgccgag cagcgcgtgc accggcgccc cgtggcgctt
      901 acccgcgatg tcccacaggg cctgctcgag cccgctcacc gcggaccaga agtccatcgc
      961 gccgcgcttg gccgcccagt cgtgataggc ccagtgcacg aagtgggtga tgtgggaggc
     1021 gtcgcggccg accaggtagc ggccgatcgc ctccacgagg gccacgacgc tggtgtcgcg
     1081 atcggcctgg gtgtagcact cgccccagcc gtgcgggccg ccgtcggtct cgaccttcac
     1141 gaagcagagg ttcttgccgc cgcccaggtc ggcgaggaac ggggtcacgc ggctcacgcg
     1201 cattgcgcga tcgtaccaga atcgggtcgc gcggcgctgg cgtggtacga ttcctccggc
     1261 tcattcgctc atgcacgccc tgacgacgac gtcaccccgc ggaacggtcc gcgcgaagct
     1321 gatgaccacg ctgttcgtgg ctcaggtctg cggcagcacc ggccactcca tcagcctggc
     1381 ggtcggcagc atcatggcgg ccagcatcac cagcaccaac acctggtcgg gcgtgccgat
     1441 cgcggtgggc gccctcggca ccgcgctcgc gagctggccg ctcgcccgtc tcatggatcg
     1501 cgcgggccgg cggccggggc tcgcgctcgg ctacgggctc gcggtggcgg gcgcggtgct
     1561 gggcctcctg ggcgtggcgg tccgcagctt ccccctgatg ctcgccggca tggcgctctt
     1621 cggcatcgcg tccacctcga acctgctcgc gcgctacgcg gccgccgacg tgagccggcc
     1681 cgagcagcgc ggccgcgcga tgggcatcat cgtgggcggc tccgcgctcg gctcgatcat
     1741 cgggccgaac ctgatggcgc ccgcgctctc cctgggcgcg cgcctcgaca tcccggacac
     1801 cgcgagcccg ttcctggtga gcgtggccgg ctacggggtc gcggcggtgc tcaccttcct
     1861 gttcctgcga cccgatccgc tcgcgctcgc ccgccgcgac gcggcggagg cgaacgcggg
     1921 ccggcccgcg ggcctcgccc gctcgctcgg cgccatcctg gccgacgtgc gcgtgcagat
     1981 cgcgctcgcc acgctctcga tcagccagtt cgtgatgatc agcaccacct cgacgtcgcc
     2041 gctctacttg cacgaccagg gccacacggt cggcaccatc ggggttgcgg tgtcgctgca
     2101 cctgggtggc atgtacgtgt cctcgccgct ctccggatgg ctctccgacc gcttcggccg
     2161 catgccggtg atcgcatccg gcgccctcat gctgatcttc gcggtggcca tcgcggggct
     2221 ggcccccggc caggccggcg ccacgatcat cctcgggctg ttcctgaacg gcatcggctg
     2281 gaacctcgcg ttcgtctccg ggagcgcgct gctcaccgac gcgctctccc ccgtcgagcg
     2341 cgcctcggtc cagggcctgg ccgacctgct catgggcctg atgggcgcca tcggctcggc
     2401 ggcgggcggg atgatcctcg ggctctgggg cttcgcgatc ctgaacgcgg tcggcgcgct
     2461 gctcgtgctg ggcccgctcg gcgtcgcgct cctgctccgg cccgcgcccg cccccgcccg
     2521 cgggtgagcc tcgccgcgct gctgcggctg cgcgaccttc gctgcgtcgc cgccggcacc
     2581 gtcttcaact cggccgggaa catgggcgag ctggtggtgc tcggctggct cgcgctcgag
     2641 ctgacgaact cgccgttcct ggtcggcgcc gcgctcggct gccgcatgct gccgctcgcc
     2701 atcgtgggcg tgccggcggg cgtgctcgcc gaccgcgtgg ctcgcccgcg cctgctcctg
     2761 atcaccgggg ccggccaggc cctgaccgcc accgcgatgg gcgcgctgac actgctcgga
     2821 gtggtgaccc tcggacagct cctgctgctg accgtcgcgg ccggcgcgct gcgcggcgtc
     2881 gagcacgcgg cgcgacagag ctacgcccac gacgtggtcg gcccgaacgc gctcgtcgac
     2941 ggcctcgcga tccttggtct ggcgatgcgc gggggctggc tggtgggctc gctcggagtg
     3001 ggcgcgctga tcgcgcaccg gggctcgggc gaggcttatc tcgcggtggc ggtcgcctac
     3061 ctgctgggcg gcctggccct gctccccgct cgcgcgctcg cgccggtcgc ggtcggcgcg
     3121 cccgactcgc tctggcgcgg cgtcaccggc ttcgcgaccg cggtgcgcga ggaccgcatg
     3181 ctgctggtgc tgatgctgct caccgccggc gcggaggtgc tgggcttcgc ccaccaggcg
     3241 ctgctgccga gcctctcgcg cgacgtgctc cacaccggcc ccgagggcct cggcgcgatg
     3301 agcgcggcgc gcgcggtggg cggcatcctg ggcctcgcgg tctcgatgcg cggcctcctc
     3361 gggggcggcg gcatggtctt cgtcggcgtg ctggtggcgt tcggggcgag cctggtggcg
     3421 ctgggcgggg cgccctacgt ggtgggcttc accggggtca tggtggtagt gctggccgcc
     3481 aacgcggcgg gcgccctcgc cgatctcatg gcccagagcc tgctccagct gagcgtgccg
     3541 catcacctgc gcgggcgcgc gggcggcgcc tgggtggtgg cgatcggctt cgcgccgctc
     3601 ggccagctcc agatcggcgc gctcgcctcg ctgctcgggg tgagcgcggc gctcggagtg
     3661 agcggggtcg ccctgatcac gctcgcctcc gccaccatct tcttctatcc acgcctccgc
     3721 gcgctctgac ctggtccggc gcggcgggcg cgttgccggt atccccggac cttggtaggc
     3781 tcggaaagca tgagccagcg agagtttgac ggcgggccgg aaggaggaca catgggagcg
     3841 agcgtgtcgg agttggcgcg ggacatcgtg gtcgccttga tccagaccgg caagatccag
     3901 ctcgacgcgg accccaagaa gcagggcgag tggctgggcg atctcttcaa g
//
LOCUS       DATMCV010001055         3404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_192552, whole genome shotgun sequence.
ACCESSION   DATMCV010001055 DATMCV010000000
VERSION     DATMCV010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192552"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="VNU03_24220"
     CDS             <1..1162
                     /locus_tag="VNU03_24220"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HWP77652.1"
                     /translation="YGLMGRTPDHVASVLTGFAGWRQLFDRGGAQFGDNVVRFHARAR
                     DEDLYVAYAIVPPRIDRGTPAHRHPEPFLHPGVVRETDTGIVIRGAHSIATSVTMADW
                     LFVSYITPLVPGDEDYAISLVVPVGAAGLRLLPRRPYATLATSVYDYPLSSRFDEIDT
                     TVVFDDVLVPWEHVFVYRSVERVTAQFHESPAHVTANFQSLVRFGVKLELLAGLAVKL
                     VEVGRGEGDPVTQATLGGDIATFCATFDALVAAAERYPLVSEGYARPHPQYVYAGMSL
                     QRRLIGDLYKTVRELAGGAFQHLPSSERSFESPETRAATERYYQSIGTPARDRVKLLK
                     LIWDFIGTEYGGRQLQYEMFYSAAQPVVNRRMFRSYDWDAARAMVDRLLSEY"
     gene            complement(1173..1919)
                     /locus_tag="VNU03_24225"
     CDS             complement(1173..1919)
                     /locus_tag="VNU03_24225"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWP77653.1"
                     /translation="MPDHTDRKLEIVTQVPESAMVVFAHPDDAEIGSGGVIARWVAAG
                     CDVTYVLCTNGDAGTADRNLAPAELAQNRAHEQRAAADFMGVRHVEMLGYPDGFLEDT
                     REFLGDVVRAIRRHRPHTVFVHDPYRMKGFQHRDHRKAGIVTTDAVYPYARDHLHFPE
                     HITRDGLEPHKVRELWYWGADEPDVIVDVTAGIERQIAALVRHQSQMPGFNVPEGVTI
                     GERVKKNAAELAAGYGFEYGGVFRRLVARR"
     gene            complement(1960..2820)
                     /locus_tag="VNU03_24230"
     CDS             complement(1960..2820)
                     /locus_tag="VNU03_24230"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HWP77654.1"
                     /translation="MREPPFPIVRHPATGARIPILVSVPHYGTQSLPHITRDDYSEPW
                     FETFAYGFADTFASDLYHDLHEHGATLLATPFSRMFVDVNRRRDDFEHHAGEVRSRRG
                     VVRTHTMREVPIFARPLAPADLEERLRALYDPYYAACEGLLDQLRSAYGYALLLDAHT
                     GSPRRMKDHQVIIGTRHGATCAPGLAAMVAAIFTGHGFEVHDNVSGYTGGNIVATFGR
                     PDTLRVHAIQLEINASLLMTTSREEFIALISRGGVPEKAERNIARVRECLREVLAALP
                     PALAALHDPD"
     gene            2877..3086
                     /locus_tag="VNU03_24235"
     CDS             2877..3086
                     /locus_tag="VNU03_24235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77655.1"
                     /translation="MRGMRRLPHAALAVSVGLALSGLAACATPSAPVPPLYTPAERRA
                     ECERHGGWWHLDDLRGDFCEPNGQM"
     gene            complement(3130..>3404)
                     /locus_tag="VNU03_24240"
     CDS             complement(3130..>3404)
                     /locus_tag="VNU03_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWP77656.1"
                     /translation="LGAAAPRTPLASPLFADLRGLPPLLIHVGSDEVLLDDSTQLADR
                     ARAAGVDATLEVWDRMIHVWHWFLPMLDEAEAAVDGIGRFIRTRAA"
BASE COUNT          466 a         1177 c         1264 g          497 t
ORIGIN      
        1 ctacgggctg atgggccgga cgcccgacca cgtggcttcg gtgctcaccg gcttcgcggg
       61 ctggcggcag ctcttcgacc ggggcggcgc gcagttcggc gacaacgtgg tccgcttcca
      121 cgccagggcg cgcgacgagg atctctacgt ggcctacgcc atcgtgccgc cccggatcga
      181 tcggggcacc ccggcccacc gccaccccga gccgttcctc cacccgggcg tggtgcgcga
      241 gaccgacacg ggcatcgtga tccgcggggc ccactcgatc gccacgtcag tcaccatggc
      301 cgactggctc ttcgtgagct acatcacccc gctcgtcccg ggcgacgagg actacgcgat
      361 ctcgctggtg gtgccggtgg gcgcggccgg gctccgcctg ctcccgcgcc ggccctacgc
      421 cacgctcgcc accagcgtct acgactaccc gctctcctcg cgcttcgacg agatcgacac
      481 cacggtggtc ttcgacgacg tgctggtgcc gtgggagcac gtgttcgtct accggagcgt
      541 ggagcgggtg accgcccagt tccacgagtc gcccgcccac gtcaccgcga acttccagtc
      601 gctggtgcgg ttcggggtga agctcgagct gctcgccggc ctcgcggtga agctggtcga
      661 ggtcggccgc ggggaaggcg atccggtcac ccaggcgacc ctcgggggcg acatcgccac
      721 cttctgcgcg acgttcgacg cgctggtggc cgccgcggag cgctatccgc tggtgagcga
      781 gggctacgcg cgcccgcacc cgcagtacgt ctacgccggt atgagcctgc agcggcggct
      841 gatcggcgac ctctacaaga cggtgcgcga gctggccggg ggcgccttcc agcacctgcc
      901 ctcgtcggag cggagcttcg agtcgccgga gacgcgcgcg gccaccgagc gctactacca
      961 gtcgatcggc acgcccgcgc gggaccgggt caagctgctc aagctgatct gggacttcat
     1021 cggcaccgag tacggaggcc gccagctcca gtacgagatg ttctactccg cggcccagcc
     1081 ggtggtgaac cgccgcatgt tccgctccta cgactgggac gcggcgcgcg cgatggtcga
     1141 ccgcctcctc tcggagtact gagcgccgcc ggctagcggc gcgcgaccag gcgacggaag
     1201 accccgccgt actcgaagcc gtagcccgcg gccagctcgg ccgcgttctt cttgacccgc
     1261 tcgccgatgg tcacgccctc cggcacgttg aagcccggca tctggctctg gtggcggacg
     1321 agcgcggcga tctggcgctc gatccccgcg gtcacgtcca cgatgacgtc cggctcgtcg
     1381 gcgccccagt accagagctc gcgcaccttg tggggctcga ggccgtcgcg ggtgatgtgc
     1441 tcggggaagt gcaggtggtc gcgcgcgtag gggtacaccg cgtcggtggt cacgatgccc
     1501 gccttgcggt ggtcgcgatg ctggaagccc ttcatgcggt acgggtcgtg gacgaacacg
     1561 gtgtgcgggc ggtggcggcg gatggcccgg accacgtccc ccaggaactc ccgcgtgtcc
     1621 tcgagaaagc cgtcggggta cccgagcatc tccacgtgcc gcacccccat gaagtcggcg
     1681 gcggcccgct gctcgtgggc ccgattctgg gccagctcgg ccggggccag gttgcggtcg
     1741 gcggtgccgg cgtcgccgtt ggtgcacagc acgtaggtca cgtcgcagcc ggcggccacc
     1801 cagcgcgcga tgaccccgcc cgagccgatc tcggcgtcgt cgggatgggc gaacaccacc
     1861 atggcggatt cggggacctg ggtgacgatc tcgagcttgc ggtcggtgtg gtcgggcatg
     1921 gcccgatctt acccggtccg ggccccgcag ggcagcaggt cagtcggggt cgtgcagggc
     1981 ggccagcgcg ggcggcaggg ccgccagcac ctcccggagg cactcccgga cgcgcgcgat
     2041 gttgcgctcg gccttctcgg ggacgccacc gcgggagatc agggcgatga actcctcgcg
     2101 gctcgtcgtc atcagcaggg acgcgttgat ctcgagctgg atcgcgtgga cgcgcagcgt
     2161 gtcgggccgg ccgaaggtgg ccacgatgtt gccgccggtg tagccgctca cgttgtcgtg
     2221 cacctcgaag ccgtggccgg tgaagatcgc ggcgaccatc gcggcgagcc cgggcgcaca
     2281 agtggccccg tggcgagtgc cgatgatcac ctggtgatcc ttcatgcggc gcgggctgcc
     2341 ggtgtgcgcg tccagcagga gcgcgtagcc gtaggcggag cgcagctggt cgaggagccc
     2401 ttcgcacgcg gcgtagtagg gatcatagag cgcccgcagg cgctcctcca agtccgccgg
     2461 cgccagaggg cgggcgaaga tcggcacctc gcgcatggtg tgggtgcgca ccaccccgcg
     2521 gcgggagcgc acctcgccgg cgtgatgctc gaagtcgtcg cggcggcggt tcacatccac
     2581 gaacatgcgg gagaacgggg tggccaggag ggtggcccca tgctcgtgca ggtcgtggta
     2641 gagatcgctc gcgaaagtgt cggcgaagcc gtaggcgaag gtctcgaacc agggctccga
     2701 gtagtcgtcg cgggtgatgt ggggcaggga ctgggtgcca tagtgcggga cgctcaccag
     2761 gatcgggatg cgcgctcccg tggccggatg ccggacgatc gggaagggcg gctcccgcat
     2821 ggccggatcg tcccagaagc gccgggaaat cgcaaggcgc ggggatgagg tgtaccgtgc
     2881 gcggtatgcg tcgcctgccg cacgcggccc tcgccgtcag cgttggcctg gccctctccg
     2941 ggctggcggc ctgcgcgacg ccgtccgccc cggtgccgcc gctctacacg ccggccgagc
     3001 ggcgggcgga gtgtgagcgg cacggcgggt ggtggcacct cgacgacctg cgcggcgact
     3061 tctgcgagcc gaacggacag atgtagccga acgggcagat gtagaaggtg cccgccggcc
     3121 ggcgcccggc taggcggccc gcgtccggat gaagcggccg atgccgtcga ccgcggcctc
     3181 ggcctcgtcg agcatcggca ggaaccagtg ccagacgtgg atcatgcgat cccacacctc
     3241 gagcgtcgcg tccacgcccg ccgcccgcgc ccggtcggcc agctgggtcg agtcgtccag
     3301 cagcacctcg tcgctgccca cgtggatgag tagcggtggc aggccgcgca ggtcggcgaa
     3361 gaggggggag gccagtggcg tccgcggcgc ggcggcgccg aggt
//
LOCUS       DATMCV010001056         2627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_723257, whole genome shotgun sequence.
ACCESSION   DATMCV010001056 DATMCV010000000
VERSION     DATMCV010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723257"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VNU03_24245"
     CDS             complement(<1..585)
                     /locus_tag="VNU03_24245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP77657.1"
                     /translation="MKILEVVGLTCHVGTTAVLDALSFTLEEGEILALCGPAGAGKTA
                     CLRCLGGALPADSGRLIFDGREVTGLGPRELAQQGLVRAGELPSPPWPLTVAQAVALA
                     LRWPQLGTAAAVLAAAWPNARARTRVAALLDRVGLSADARRRRDGLPAAAMGRLGLAQ
                     ALAQRPRLLLLDEPLGRLPADDRPAAARLIDEIRA"
     gene            complement(600..1916)
                     /locus_tag="VNU03_24250"
     CDS             complement(600..1916)
                     /locus_tag="VNU03_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWP77658.1"
                     /translation="MARKPAAPRTGKTRGREPDAVDPKHRWDRPIQAPGHTQVDFEER
                     VDFRRLHDYRLGRTRGALAKSGLGALLLFDQYNIRYISGTVIGEWARDKLTRWCLLTG
                     NGEPWVWDFGSAARHHRLSTPQLPPNQCLAGMVGMRGAVSPKAGLFEQAAREIRDILV
                     REGVADMPLGIDVVEPPFLFALQKLGIEVRDGQQVMLEARVIKNVDELVLLNMAAAMG
                     DGVYQDIFEALKPGVRENDIVALASKRLYEMGSDCVEAINAISGERCSPHPHNFTDRI
                     IRPGDQAFFDIIQSYVGYRTCYYRTFSVGRATSPQQSAYKKAREWMDSAIALIKPGVG
                     TDRIARAFPRAQDIGFESEMAAFGLNFCHGLGLGLHERPIISRLNSFDDPMELREGMV
                     FAVETYCPATDGTSAARIEEEVIVTPRGAQIITLFPANELPIANRY"
     gene            2042..>2627
                     /locus_tag="VNU03_24255"
     CDS             2042..>2627
                     /locus_tag="VNU03_24255"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HWP77659.1"
                     /translation="MPSTKGLNFFLEDRSFQLLCEQVMGPEVFALARPHLVALGEVAG
                     DELDALAAEADRNPPVLRPFDAQGRRVDEVVKHPAYRRMEEIAFGRFGLAAMAHRPGV
                     LGFPDRAPQVVKYALSYVFAQSEFGLLCPVNMTDSCARMLGAFGSEELKARYLPHLTA
                     TDPAALWQGTQWMTERTGGSDVGTSTTVARRDADG"
BASE COUNT          362 a          904 c          990 g          371 t
ORIGIN      
        1 ggcgcgaatc tcatcgatca ggcgggccgc ggccggccgg tcgtcggccg gcaggcggcc
       61 cagcggctcg tccaggagca gcaggcgggg ccgctgggcg agggcctggg cgaggccgag
      121 tcgccccatc gccgccgcgg gcaggccgtc gcgccgccgc cgggcgtccg ccgagaggcc
      181 gacccggtcg agcagcgccg ccacgcgggt gcgggcgcgg gcattgggcc acgcggcggc
      241 caggaccgcg gcggcggtgc cgagctgcgg ccagcgcagc gcgagggcca ccgcctgcgc
      301 gaccgtgagc ggccagggcg gcgagggtag ctcgccggcg cggaccagcc cctgctgggc
      361 gagctcccgc gggcccagcc cggtgacctc gcggccatcg aagatgagcc ggcccgaatc
      421 ggcggggaga gcgccgccga ggcagcggag acaggcggtc ttgcccgcgc cggcgggccc
      481 gcacagggcc aggatctcgc cttcctcgag cgtgaaggag agcgcgtcga ggacggcggt
      541 cgtccccacg tggcaggtga gcccgacgac ctcgaggatc ttcatgcggc gaggatagct
      601 cagtaccggt tggcgatcgg cagctcgttg gccgggaaga gggtgatgat ctgggcgccc
      661 cggggcgtca cgatcacttc ctcctcgatg cgcgccgccg aggtgccgtc ggtcgcgggg
      721 caatacgtct ccaccgcgaa gaccatgccc tcccgcagct ccatcgggtc gtcgaaggag
      781 ttgagccgcg agatgatcgg tcgctcgtgc aatccgaggc cgaggccgtg acagaagttg
      841 aggccgaacg cggccatctc gctctcgaag ccgatgtcct gggcccgcgg gaaggcgcgc
      901 gcgatgcggt cggtgcccac gccgggcttg atcagggcga tggccgagtc catccactcg
      961 cgggccttct tgtacgcgct ctgctgcggt gaggtggcgc gccccacgct gaaggtgcgg
     1021 taatagcagg tgcggtagcc cacgtaggac tggatgatgt cgaagaaggc ctggtcgccc
     1081 gggcggatga tccgatcggt gaagttgtgc gggtgagggg agcagcgctc gcccgagatg
     1141 gcgttgatgg cctccacaca gtccgacccc atctcgtaga gccgcttgct ggcgagggcc
     1201 acgatgtcgt tctcgcgcac cccgggtttg agcgcctcga agatgtcctg gtagaccccg
     1261 tcgcccatcg cggcggccat gttgagcagc accagctcgt cgacgttctt gatcacgcgc
     1321 gcctccagca tcacctgctg gccgtcgcgc acctcgatcc cgagcttctg cagcgcgaac
     1381 aggaacggcg gctcgacgac gtcgatgccg agcggcatgt cggccacgcc ctcgcgcacg
     1441 aggatgtcgc ggatctcgcg cgcggcctgc tcgaagaggc cggccttggg cgagaccgcc
     1501 ccgcgcatgc ccaccatgcc ggccaggcac tggttggggg gcagctgcgg ggtggagagg
     1561 cggtggtggc gcgcggccga gccgaagtcc cacacccagg gctcgccgtt gccggtgagc
     1621 aggcaccagc gggtcagctt gtcgcgcgcc cactcgccga tgacggtgcc cgagatgtag
     1681 cggatgttgt actggtcgaa gagcaggagc gcgccgagtc ccgatttcgc gagcgcgccg
     1741 cgggtgcggc cgaggcggta gtcgtggagg cggcggaagt ccacgcgctc ctcgaaatcg
     1801 acctgggtgt ggccgggggc ctggatgggc cggtcccacc ggtgcttcgg gtcgaccgcg
     1861 tcgggctccc ggccccgggt cttcccggtc cggggcgctg ccggcttgcg cgccatgtcc
     1921 gcctcctgtg atccgtggtg aacgacgacg gcgtcactcc taccacagac gcgtggttcg
     1981 cgatagactc gggtgcacga tgacgccgcc gccgtcgttc ccaccgcccg cgcaggacgc
     2041 gatgccgagc acgaaaggcc tgaacttctt cctggaggat cggagcttcc agctgctctg
     2101 cgagcaggtc atgggcccgg aggtcttcgc gctcgcgcgc ccgcatctgg tggcgctggg
     2161 cgaggtggcc ggcgacgagc tcgacgcgct ggccgcggag gccgaccgca acccgccggt
     2221 gctgcgcccg ttcgacgcgc agggccgccg ggtggacgag gtggtgaagc atcccgcgta
     2281 tcgccggatg gaggagatcg cgttcggccg cttcgggctc gccgcgatgg ctcaccggcc
     2341 cggcgtgctc ggtttccccg accgcgcgcc gcaggtcgtg aagtacgcgc tctcgtacgt
     2401 cttcgcgcag agcgagttcg gcctgctctg tccggtcaac atgaccgact cgtgcgcgcg
     2461 catgctgggg gccttcggca gcgaggagct caaggcgcgc tacctgccgc acctcaccgc
     2521 caccgatccg gccgcgctct ggcaaggcac ccagtggatg accgagcgca ccggcggctc
     2581 cgacgtcggc acctccacca cggtggcccg gcgcgacgcg gacgggg
//
LOCUS       DATMCV010001057         4851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_523805, whole genome shotgun sequence.
ACCESSION   DATMCV010001057 DATMCV010000000
VERSION     DATMCV010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523805"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VNU03_24260"
     CDS             <1..835
                     /locus_tag="VNU03_24260"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HWP77660.1"
                     /translation="GAALVLHAWGTISAAMALYALWPSAWTLGLAVVVIGGRQLGLVV
                     LMHETSHWLLFSSLRANTWVGTWLCAAPMGEDLRAYRRRHHLHHRLTQRPDDPDLELS
                     ASLPLSRGQSALAVLGDLSGVTAAAEVLGWRPGPDGLAAAWRRARAPLIANTVLAAAL
                     VAIGGWSLYLLTWVLPWATWYRVVTRIRNTVEHGLVPGAEDPLRSARSVGAGRLTRAL
                     VAPYGVNYHLEHHLLVFVPCWKLRQVHAMLLAKGYGSRMERSPSYLEVLGRATAGRPP
                     R"
     gene            complement(864..1049)
                     /locus_tag="VNU03_24265"
     CDS             complement(864..1049)
                     /locus_tag="VNU03_24265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77661.1"
                     /translation="MNDLQGHLLAIAHEVKDLRDRVAGIDQLQAELARLRAERDELQD
                     VLARIGTLANRVRPGVG"
     gene            1161..1946
                     /locus_tag="VNU03_24270"
     CDS             1161..1946
                     /locus_tag="VNU03_24270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP77662.1"
                     /translation="MKPREETIAVGPTRVQLWIGGEGDPLLVLHGAGGNRGWLRWMDA
                     VAERHTIYAPTHPGFGRSDAADWMEGIDDLARFYLWFLDVMGLRRVHLLGHSIGGWTA
                     AEMATMCPDVIDRLVLVAPTGLKPEVGEILDIFYYPPEELFTHTVFDASTIPERERLF
                     GRPPTPEEAEIALRNREMAARLTWKPYMFNPRLPHFLPRVTAPTLVLWGREDGIVPVI
                     CGEQYRRLLPNATLRVLERCGHLPLIEKPDEFAGLVLDFLRRA"
     gene            1948..3078
                     /locus_tag="VNU03_24275"
     CDS             1948..3078
                     /locus_tag="VNU03_24275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77663.1"
                     /translation="MKLYYFSEMPHHEYPDAEGDKYPSLRLAFPNTFFSPEIAQRNYQ
                     RYLDEYDAADQAGFDGLMINEHHSTPSCVNVSANMTAGILARTTRNAKLLILGNILPI
                     QDNPVQMAEQVAMADLISGGRVLSGFVRGVGVESWWANSNPVHNRERFEECHDLIIKC
                     WTTPGPFRWEGRHYHFRHVNPWCLPLQKPHPPIWVPGTASPETAVWAGRMGYTYVPFL
                     VSFEVARELFDFYRQGAAEAGRTVTEDKLGFLICAVTADTKARALEAGRHFVWRMGPT
                     LRGPIEYFSPVGMRSREASRFALRARSVSLSKMSYEELVENYYIVAGTPDEVADRFAR
                     VHRELGIGHLLLEAQESRMDHATTMRSIDLMGKSVIPAMGRL"
     gene            complement(3062..3964)
                     /locus_tag="VNU03_24280"
     CDS             complement(3062..3964)
                     /locus_tag="VNU03_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWP77664.1"
                     /translation="MERDFVGYADTPPIVPWPDGARLAVQVSVSYEEGAEYSLLDGPR
                     RETMGEVPSPVPPDRRDLFNESFFEYGSRVGVWRILDTCRRAGVPASWWACARALERN
                     PRVARALVDAGHEICGHGYGWEEFHGANREEERDNIRRTVESLVRTTGQRPVGWFTRY
                     ACSPSTRELLAEEGGFLYDSLGLNDDLPYYVPVQGRPWLVVPYTFEVNDARFWRGGLT
                     SVGAFTEYMRAAFDCLYAEAQRVPRMMSIGLHCRIAGTPARSRAFVEFLEYARRFPGV
                     WFARRVDIARWWLEHQPPALRAAP"
     gene            complement(3981..4676)
                     /locus_tag="VNU03_24285"
     CDS             complement(3981..4676)
                     /locus_tag="VNU03_24285"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWP77665.1"
                     /translation="MGQYEQHVFVCTSGETCPEQGDTEKFVKILRERAKQAGRTADVR
                     INKAGCFSQCGRGPMIVFYPDDVWYAGVTESDLEEIFTSHIVDGRPVERLRYDPGKPG
                     ANKLSEAELAEIKRAHAARTQTPAPADPARAWQRVGATAEVPANGMKEFSVGGVNVLV
                     VHTGSAYVAFQALCPHETIPLEQGMHDGSVLTCLEHMWQFDLRTGAPLGDALEGLRGY
                     PLREEAGLLYVAL"
BASE COUNT          670 a         1751 c         1702 g          728 t
ORIGIN      
        1 cggcgcggcc ctcgtgctcc acgcgtgggg caccatctcg gcggcgatgg cgctctacgc
       61 gctctggccg tccgcctgga cgctcgggct cgcggtggtg gtgatcggcg gccggcagct
      121 cgggctcgtg gtgctcatgc acgagacgtc tcactggctc ctcttctcct cgttgcgggc
      181 caacacctgg gtcggcacct ggctctgcgc cgcgccgatg ggggaggatc ttcgcgcgta
      241 ccggcgccgg caccatctgc accaccggct cacccagcgg cccgacgatc ccgacctcga
      301 gctctcggct tcgctgccgc tgtcccgcgg acagtccgcg ctcgccgtcc tgggcgatct
      361 gagcggcgtc accgcggccg ccgaggtgct cggctggcgc ccggggccgg acggcctggc
      421 cgcggcgtgg cgccgcgcgc gggcgccgct gatcgccaac acggtcctgg ccgccgcgct
      481 cgtggcgatc ggcgggtggt cgctctacct gctcacctgg gtcctgccgt gggccacgtg
      541 gtaccgggtg gtgacgcgca tccggaacac cgtcgagcac gggctggtgc cgggcgcgga
      601 ggacccgctc cggagcgccc gcagcgtcgg agccggacga ctcacgcggg cgctcgtggc
      661 gccctacggg gtgaactacc atctggagca ccatctgctc gtgttcgtgc cctgctggaa
      721 gcttcgccag gtccacgcga tgctgctggc caagggctac gggtcgcgga tggagcggtc
      781 acccagctat ctcgaggttc tcggccgcgc caccgccggc cggccgccgc gctagtcgcg
      841 gtactcgcgg cgcccgctgc cggtcatccc accccgggac ggacccggtt cgcgagggtg
      901 ccgatgcggg ccaacacgtc ctgcagctcg tcacgctccg cccggagccg cgcgagctcg
      961 gcctggagct gatcgatacc cgccacccgg tcccgcaggt ccttcacctc gtgtgcgatc
     1021 gccaacaggt gcccttgcag gtcgttcacg tgatcgatcc tttcgctgaa ttgatcgccc
     1081 gtacctgaca gtgtacgggc gcccctgccg tcgcccatcg catattgtcc gccgtggcca
     1141 cttgaggcta tgctcccccg gtgaagcctc gcgaggagac gatcgcggtc gggccgaccc
     1201 gggtgcaatt gtggatcggc ggcgagggtg atcccctgct ggtccttcac ggggcgggcg
     1261 gcaaccgggg ctggctccgc tggatggacg cggtggccga gcgccacacg atctacgccc
     1321 ccacccatcc cggcttcggc cgctccgacg cggcggactg gatggagggc atcgacgacc
     1381 tcgcgcgctt ctacctctgg ttcctcgacg tgatggggct ccgccgcgtg cacctcctcg
     1441 ggcactccat cggcgggtgg accgcggccg agatggccac catgtgtccg gacgtcatcg
     1501 atcgcctggt gctggtcgcg ccgaccgggc tcaagcccga ggtgggcgag atcctcgaca
     1561 tcttctacta cccgccggag gagctgttca cgcacaccgt cttcgacgcg tcgaccatcc
     1621 cggagcgcga gcggctcttc ggccggccgc ccaccccgga ggaggcggag atcgccctcc
     1681 gcaaccggga gatggccgcg cgcctcacct ggaagcccta catgttcaac ccgcggctcc
     1741 cccacttcct cccccgggtg accgcgccca cgctcgtgct gtgggggcgc gaggacggga
     1801 tcgtccccgt gatctgcggc gagcagtacc gccgcctgct gcccaacgcg acgctgcgcg
     1861 tgctcgagcg ctgcggccac ctgcccctga tcgagaagcc ggacgagttc gcggggctcg
     1921 tgctcgattt cctccggagg gcgtgaggtg aagctctact acttcagcga gatgccgcac
     1981 cacgagtacc ccgacgcgga gggggacaag tacccctcgc tgcggctcgc cttccccaac
     2041 acatttttct cgcccgagat cgcccagcgc aactaccagc gctacctcga cgagtacgac
     2101 gcggcggacc aggcgggctt cgacgggctc atgatcaacg agcaccactc gacgccctcg
     2161 tgcgtgaacg tctccgccaa catgaccgcg ggcatcctcg cccggacgac tcgcaacgcg
     2221 aagctgttga tcctgggcaa catcctgccg atccaggaca acccggtgca gatggccgag
     2281 caggtggcga tggccgatct catctcgggc gggcgcgtgc tgagcggctt cgtgcgcggc
     2341 gtgggggtgg agagctggtg ggcgaacagc aacccggtcc acaaccgcga gcggttcgag
     2401 gagtgccacg acctgatcat caagtgctgg accaccccgg gcccgttccg gtgggagggc
     2461 cggcactacc acttccgcca cgtcaatccg tggtgcctgc cgctgcagaa gccgcacccg
     2521 ccgatctggg tgccggggac cgcgagcccg gagaccgcgg tgtgggccgg gcgcatgggg
     2581 tacacctacg tccccttcct ggtctcgttc gaggtggcgc gcgagctctt cgacttctac
     2641 cgccagggcg cggcggaggc cgggcggacg gtgaccgagg acaagctcgg cttcctgatc
     2701 tgcgcggtca ccgcggacac caaggcccgc gcgctcgagg ccggccgaca cttcgtgtgg
     2761 cgcatggggc cgaccctgcg ggggccgatc gagtacttct ccccggtggg catgcgctcg
     2821 cgcgaggcga gccggttcgc cctccgcgcg cgctcggtct cgctgtcgaa gatgagctac
     2881 gaggagctgg tggagaacta ctacatcgtg gccggcacgc ccgacgaggt ggcggaccgc
     2941 ttcgcgcggg tgcaccggga gctcgggatc ggccacctgc tgctcgaggc ccaggagtcg
     3001 cgcatggacc acgccaccac catgagatca atcgatttga tgggcaagag cgtgatcccg
     3061 gctatggggc ggctctgagc gccgggggct gatgctcgag ccaccagcgg gcgatgtcca
     3121 cgcgccgggc gaaccagaca cccgggaagc gccgcgcgta ctcgaggaac tccacgaagg
     3181 cgcgtgaccg ggccggggtg cccgcgatcc ggcagtgcag gccgatcgac atcatccgcg
     3241 gcacccgctg ggcctcggcg tagaggcagt cgaaggccgc gcgcatgtac tcggtgaagg
     3301 cgcccacgct cgtgagcccg ccccgccaga agcgcgcgtc gttgacctcg aacgtgtagg
     3361 gcaccaccag ccagggccgc ccctgcaccg gcacgtagta gggcaggtcg tcgttcaggc
     3421 ccaggctgtc gtagaggaag ccgccctcct cggcgaggag ctcgcgggtg ctcgggctgc
     3481 acgcgtagcg ggtgaaccaa cccacgggcc gctgaccggt ggtgcgcacc agcgactcca
     3541 cggtgcgccg gatgttgtcc cgctcctcct cgcggttcgc gccgtggaac tcctcccagc
     3601 cgtagccgtg gccgcagatc tcgtggcccg cgtccaccag ggcgcgtgcc acgcgcgggt
     3661 tgcgctcgag cgcccgggcg caggcccacc aggaggcggg cacgcccgcg cgccggcagg
     3721 tgtcgaggat gcgccacacc ccgacccggc tgccgtactc gaagaacgac tcgttgaaga
     3781 gatcgcggcg gtccgggggc accggcgacg gcacctcgcc catggtctcg cggcggggcc
     3841 cgtcgaggag cgagtactcg gcgccctcct cgtaggagac cgagacctgg accgcgaggc
     3901 gggcgccgtc cggccagggg acgatcggcg gggtgtcggc gtatccgacg aaatcgcgct
     3961 ccatggcggc gcgggccggt ctagagcgcg acgtacagca gtcccgcctc ctcccggagc
     4021 gggtagccgc ggaggccctc cagggcgtcg ccgagcgggg cgccggtccg gaggtcgaac
     4081 tgccacatgt gctcgaggca ggtcaggacc gacccgtcgt gcataccctg ctccagcggg
     4141 atggtctcgt gcgggcacag cgcctggaag gccacgtagg cggagccggt gtggacgacg
     4201 aggacgttga cgcccccgac cgagaactcc ttcatgccgt tcgcgggcac ctcggcggtc
     4261 gcgcccacgc gctgccacgc ccgcgccgga tcggccgggg ccggcgtctg ggtgcgggcc
     4321 gcgtgggccc gcttgatctc ggccagctcg gcctcgctca gcttgttcgc gcccggcttt
     4381 ccggggtcgt agcggaggcg ctcgaccggc cggccgtcca cgatgtggga ggtgaagatc
     4441 tcctccagat ccgactccgt gacccccgcg taccagacgt cgtccgggta gaacacgatc
     4501 atcggccccc gcccgcactg cgagaagcac cccgccttgt tgatgcgcac gtcggcggtc
     4561 cggccggcct gcttggcgcg ctcgcggagg atcttgacga acttctcggt gtcgccctgc
     4621 tcgggacagg tctcgccgct cgtgcacacg aagacgtgct gctcgtactg acccatgacc
     4681 gctcctttcg ggcttagctg aacttgtcgt atcggatgtc ggcgggcgac agtctcgcct
     4741 cgcggagcag caaacgcaag ctcgcgtcga ccatcggggg tgggcccgcc acgtaggcgc
     4801 ggacgccggc gaagcggccg gccatgcgcg cgccggccac ctcgtgcacg a
//
LOCUS       DATMCV010001058         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_192663, whole genome shotgun sequence.
ACCESSION   DATMCV010001058 DATMCV010000000
VERSION     DATMCV010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192663"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VNU03_24290"
     CDS             <1..1083
                     /locus_tag="VNU03_24290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77666.1"
                     /translation="LAVPNNFGAIYPKEIAEKFAPEVKVTEYVGTGPYKLAEWKPDQY
                     IRMVRFDDYKSRNEKPNGYGGGKTAWLDEIRWVPVPEVATRVAQVETGELDFADDLNL
                     DAYDRLKGNANARPIVSKPYYWLVAVFNKKEGLMTNQKLRQAWQAAIDIEPIMKNVAG
                     GKKEFYRMDSSLAPAEITAWHTKMSGLPWNEHNRDKAKRLLQEAGYKQEPIRFMTTQE
                     YKWMYDFALLSKQQLEDAGFNIDLQVVDWATLVKRRNNSKEYDVFTTGMGAFYDPTHH
                     IYLTPSWPGWTTDEGILKIQQELARETDPKKRMALWEQQTRQFYEKVPVIRYGDLFGL
                     RAARNTVKGFNESVERVRFYNVWLEK"
     gene            1175..2119
                     /locus_tag="VNU03_24295"
     CDS             1175..2119
                     /locus_tag="VNU03_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77667.1"
                     /translation="MLSYLVRRLLAVIPVMAVVVTVVFLLIHLIPGDPVSVMLGPDAT
                     PTQIEGTRKALGLDGPLLGQLLGFYGRVLRGDLGQSYFLDRPVTTAILERAEPTLVLM
                     ACALLVAVAIGVPSGIVAAAHRGSIWDRVLMIGSLLGVCVPGFWLSLNFIYLFAVRLG
                     WLPAAGYASLFSEPGTALRYMVLPAVSLGFNQSALIARISRSCMLEALQQDYVRTARA
                     KGLSYRTVTYVHAFRNALVPVVTVIGVTTAVLIGGAVITEIVFNIPGLGRLVISAILR
                     RDYPVVQGVVLVTAATYVLINLGVDLLYAFIDPRIRYD"
     gene            2112..2963
                     /locus_tag="VNU03_24300"
     CDS             2112..2963
                     /locus_tag="VNU03_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77668.1"
                     /translation="MTEVLTERQRWVRRLTGNGAVMTGAAILLVLVAAALLADVVSTH
                     NPTRLVPANRLQPPSAQHYLGTDEFGRDVYSLVLHGSRVSLLVGLTTMLLTSAGGIVI
                     GLTAGYARRLDVPVMRCMDGLMAFPAILLAIALMAARGPGVWNVILALSVVYMPRTAM
                     LIRSTVLSLRELDYIQAARALGRHDGAIALRHILPNCVGPLLVQGSFVFAYAILAEAI
                     LGFLGVGVPPYVPSWGNVIASGKNVIREAFWVSLFPGLALTIAGLSLNLLGDGLRDVL
                     DPRLRVR"
     gene            2982..>3602
                     /locus_tag="VNU03_24305"
     CDS             2982..>3602
                     /locus_tag="VNU03_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWP77669.1"
                     /translation="MTHRTIGMLSGLLMALVVCVLAPSAARAHRPTDPPHQLYQMGDL
                     TLESGQVIRDFAISYVTHGTLNAKKSNAILMVTALSGNHHRLDFLIGPGKALDPGKYF
                     IVCTDAISNGLTTSPSNSKAQPRMQFPRFAMRDMVQSQHRLLTEKLGITRVAAVVGPS
                     MGGMQALQWGVSHPDFMDSLVAIVPLARTPAWSTVVVDASREAIMLD"
BASE COUNT          565 a         1266 c         1196 g          575 t
ORIGIN      
        1 ctcgcggtgc ccaacaactt cggcgcgatc tacccgaagg agatcgcgga gaagttcgcc
       61 cccgaggtga aggtcaccga gtacgtgggc accggcccct acaagctcgc ggagtggaag
      121 cccgatcagt acatccgcat ggtccgcttc gacgactaca agtcgcgcaa cgagaagccc
      181 aacggctacg gcgggggcaa gaccgcgtgg ctcgacgaga tccggtgggt gccggtgccg
      241 gaggtggcga cccgggtggc ccaggtggag accggcgagc tcgacttcgc ggacgacctg
      301 aacctcgacg cctatgaccg gctgaagggc aacgccaacg cgcgtcccat cgtctccaag
      361 ccgtactact ggctcgtggc cgtcttcaac aagaaggaag gcctgatgac gaaccagaag
      421 ctgcggcagg cctggcaggc cgccatcgac atcgagccga tcatgaagaa cgtggccggg
      481 ggcaagaagg agttctaccg gatggacagt agcctggccc cggccgagat caccgcctgg
      541 cacaccaaga tgtccgggct gccgtggaac gagcacaacc gggacaaggc caagcggctc
      601 ctgcaggagg cgggctacaa gcaggagccg atccgcttca tgaccaccca ggagtacaag
      661 tggatgtacg acttcgcgct gctcagcaag cagcagctcg aggacgccgg cttcaacatc
      721 gacctgcagg tggtggactg ggcgaccctg gtgaagcggc gcaacaactc caaggagtac
      781 gacgtcttca ccaccggcat gggcgccttc tacgacccga cccatcacat ctacctcacc
      841 ccgagctggc cgggctggac caccgacgag ggcatcctga agatccagca ggagctggcc
      901 cgggagaccg atcccaagaa gcgcatggcg ctctgggagc agcagacccg gcagttctac
      961 gagaaggtgc cggtcatccg ctacggcgac ctcttcggcc tccgcgcggc ccgcaacacg
     1021 gtgaagggct tcaacgagtc ggtcgagcgc gtgcgcttct acaacgtctg gctggagaag
     1081 tagctcggcg cttggcgagg tgcagccgaa ggcgagccga gcggataaat aagtgaggcg
     1141 gcagcccgtt cgcggggcgg cctgacccgc cctcatgctt tcctacctcg tccgtcgcct
     1201 cctcgccgtc atcccggtga tggcggtagt ggtgacggtc gtgttcctgc tcatccacct
     1261 gatccccggc gatccggtga gcgtgatgct ggggcccgac gcgacgccga cccagatcga
     1321 gggcacccgc aaggcgctcg ggctcgacgg gccgctgctc ggccagctcc tggggttcta
     1381 cggccgcgtg ctgcgcggtg acctcggcca gtcctacttc ctggaccggc cggtgaccac
     1441 cgcgatcctg gagcgcgccg agccgaccct ggtgctgatg gcgtgtgccc tcctggtggc
     1501 ggtggcgatc ggggtgccct ccggcatcgt ggccgccgcc caccggggct cgatctggga
     1561 ccgcgtgctg atgatcggct ccctgctcgg ggtctgcgtt cccggcttct ggctgagcct
     1621 caacttcatc tacctcttcg cggtgcgcct gggctggctg cccgccgcgg gctacgcgtc
     1681 gctcttcagc gagccgggca ccgcgctgcg ctacatggtg ctccccgcgg tctcgctcgg
     1741 cttcaaccag tcggccctca tcgcccgcat cagccgctcg tgcatgctcg aggcgctgca
     1801 gcaggactac gtgcgcaccg cgcgagccaa ggggctgtcg taccgcaccg tcacctacgt
     1861 gcacgccttc cgcaacgcgc tggtgccggt ggtgaccgtg atcggggtga ccaccgcggt
     1921 gctgatcggc ggcgcggtga tcaccgagat cgtcttcaac atccccggcc tcggccgcct
     1981 cgtgatctcg gcgatcctgc gccgggacta cccggtggtg cagggggtgg tgctggtcac
     2041 cgccgcgacc tacgtgctga tcaacctggg ggtcgacctg ctctacgcct tcatcgaccc
     2101 gcgcatccgc tatgactgag gtcctgaccg agcggcagcg ctgggtgcgc cgcctgaccg
     2161 gcaacggcgc ggtgatgacc ggcgccgcga tcctgctcgt cctggtggcc gccgcgctgc
     2221 tggccgacgt ggtctccacc cacaacccca cgcgcctcgt gccggccaac cggctccagc
     2281 ccccgagcgc gcagcactac ctcggcaccg acgagttcgg ccgcgacgtc tacagcctgg
     2341 tgctgcacgg gagccgggtg tccctgctgg tggggctcac caccatgctg ctcacctccg
     2401 cgggcggcat cgtcatcggc ctcaccgcgg gctacgcgcg ccggctcgac gtgccggtga
     2461 tgcgctgcat ggacggcctg atggcgttcc cggccatcct gctggcgatc gcgctcatgg
     2521 cggcccgggg ccccggggtg tggaacgtga tcctggccct ttcggtggtc tacatgccgc
     2581 gcaccgcgat gctcatccgc agcacggtgc tgagcctgcg cgagctggac tacatccagg
     2641 ccgcccgcgc gctgggccgg cacgacggcg cgatcgccct gcgccacatc ctgccgaact
     2701 gcgtgggccc gctgctggtg caggggagct tcgtgttcgc ctacgcgatc ctcgccgagg
     2761 ccatcctggg cttcctcggg gtgggcgtgc cgccctacgt gccctcgtgg ggcaacgtga
     2821 tcgcgagcgg caagaacgtc atccgcgagg cgttctgggt gagtctcttt cccggcctgg
     2881 ccttgaccat cgccgggctg agcctcaatc tcctgggcga tggattgcga gacgtgctgg
     2941 acccgcggct ccgagtgcgc tagaaagggg ggcaccgaat aatgacacac cgcacgatcg
     3001 ggatgctgag cggcctgctg atggcgctgg tcgtgtgcgt gctcgctccc tccgcggccc
     3061 gggcgcaccg gccgaccgac ccgccgcacc agctctacca gatgggtgac ctcacgctcg
     3121 agagcgggca ggtcatccgc gacttcgcca tctcctacgt gacccacggc acgctgaacg
     3181 cgaagaaatc caatgcgatc ctgatggtca ccgcgctcag cggcaaccac catcgcctcg
     3241 atttcctgat cggccccggc aaggcgctcg accccggcaa gtacttcatc gtctgcaccg
     3301 acgcgatctc gaacgggctc accacctcgc ccagcaacag caaggcacag ccccggatgc
     3361 agttcccgcg cttcgcgatg cgggacatgg tccagtcgca gcaccggttg ctgaccgaga
     3421 agctcgggat cacccgggtc gccgcggttg tcggcccctc gatgggcggg atgcaggcgc
     3481 tccagtgggg ggtgagccac cccgacttca tggacagcct ggtggccatc gtgccgctgg
     3541 cccgcacccc ggcctggagc acggtggtgg tcgacgcctc ccgcgaggcg atcatgctcg
     3601 ac
//
LOCUS       DATMCV010001059         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_257867, whole genome shotgun sequence.
ACCESSION   DATMCV010001059 DATMCV010000000
VERSION     DATMCV010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257867"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VNU03_24310"
     CDS             complement(<1..1117)
                     /locus_tag="VNU03_24310"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HWP77670.1"
                     /translation="MTGGDGARALLRDGPRVINVGLESFALDLDARGVPVLHVAWRPP
                     AGGDVAAARRLAALDDGAVAATIQAANEAVLATMTTAHPVLVDCRPAREAMGLEERLV
                     LHAGPPLAWARACPTMQAAVLCAIRYEGWAPDDATARALVERGAVALAPCHSRGAVGP
                     MTGLITASMPVFVVENRDGGNRAFATINEGLGAVLRFGANDERVIRRLEWLASEAGPV
                     LGAALRAGGGVDLRALMGQALRMGDEMHQRNMAASALLSRLLMPAIARATTDAGIIAR
                     LADFIGGNDQFFLNLAMAAGKATTDPCVGHAGSTLVVTMARNGTDFGIRVAGLGDRWL
                     TAPVNTPSGLYFAGFGPADANPDIGDSAIVETIGLGGF"
     gene            complement(1114..>2258)
                     /locus_tag="VNU03_24315"
     CDS             complement(1114..>2258)
                     /locus_tag="VNU03_24315"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77671.1"
                     /translation="LNVILFSDNVSLADEIALKRQATARGLLLMGPDCGTAYVADVPL
                     GFANAVPPGCVGLVAASGTGLQQVATLLAGRGRGLSHAIGVGGRDMSEAVGGIMTLAA
                     LDLLAANAATELIVVIGKPPAPAVGERIRAKLAGIGKPVVVCLLGRDVIVGREGPVTT
                     VATLEDAALAADAALAGTAWQPGVFSVPDVSRRIAEARRDLSSGQSAVLGLYAGGTLA
                     HESVLILEPLLGPVASNLTGGGGRHRVLDLGADEFTVGRAHPMLDPTTRVELIGRASE
                     QADLAVLLLDVVLGHGAAADPAGDVAPSLEAARTAARARGRGLAVVASVVGTARDPQG
                     LAAQVARLEAAGAWVLPSNAQAARAAAAIVGGEAVVTALLRPGARA"
BASE COUNT          282 a          869 c          864 g          243 t
ORIGIN      
        1 cgaagccgcc gaggccgatg gtctccacga tcgcgctgtc gccgatatcg gggttggcgt
       61 ccgcggggcc gaagcccgcg aagtagagcc cgctcggcgt gttgaccggc gcggtgagcc
      121 agcggtcgcc gagcccggcc acgcggatgc cgaagtcggt gccgttgcgc gccatcgtga
      181 ccaccagggt ggagcccgcg tggcccacgc acgggtcggt ggtggccttc cccgcggcca
      241 tggccaggtt gaggaagaac tggtcgttgc cgccgatgaa gtcggcgagc cgcgcgatga
      301 tccccgcgtc ggtcgtggcg cgggcgatgg cgggcatcag gaggcgcgag aggagcgcgg
      361 aggccgccat gttgcgctgg tgcatctcgt cgcccatgcg gagggcctgg cccatgagcg
      421 cgcgcaggtc cacgccgccg cccgcgcgca gcgccgcgcc cagcaccgga cccgcctcgg
      481 aggcgagcca ctcgagccgc cggatcaccc gctcgtcgtt ggcgccgaag cggagcaccg
      541 cgccgagccc ctcgttgatg gtcgcgaagg cccggttgcc cccgtcgcgg ttctccacca
      601 cgaacaccgg catcgacgcg gtgatgagcc cggtcatcgg ccccaccgcg ccacggctgt
      661 ggcagggcgc gagcgcgacc gcgccgcgct cgaccagggc ccgcgccgtc gcgtcgtcgg
      721 gcgcccagcc ctcgtagcgg atggcgcaca gcaccgcggc ctgcatggtc gggcacgcgc
      781 gggcccaggc gagcggcggc cccgcgtgga gcacgaggcg ctcctcgagg cccatggcct
      841 cgcgcgcggg ccggcagtcg acgagcaccg gatgggccgt ggtcatggtg gccagcaccg
      901 cctcgttcgc ggcctggatg gtcgcggcca ccgcgccgtc gtcgagcgcg gccagccgcc
      961 gcgcggccgc cacgtcgccg cccgcaggcg gccgccaggc cacgtggagc accgggacgc
     1021 cgcgcgcgtc cagatcgagg gcgaagctct ccaggcccac attgatgacg cgcggcccgt
     1081 cgcggagcag ggcgcgcgcc ccgtcgcctc cggtcacgcg cgagctcccg gccggagcag
     1141 cgcggtgacc accgcctcgc cacccacgat ggcggccgcg gcccgggcgg cctgcgcgtt
     1201 cgacggcagc acccacgcgc cggcggcctc gagccgcgcc acctgggcgg ccagcccctg
     1261 gggatcgcgc gccgtgccca ccacggaggc gacgaccgcg aggccgcggc cgcgcgcgcg
     1321 ggccgcggtc cgggccgcct cgagcgaggg ggccacgtcg ccggcgggat cggcggcggc
     1381 cccgtgcccg agcaccacgt cgagcaggag gaccgctagg tcggcctgct cgctcgcgcg
     1441 gccgatcagc tccacgcggg tggtcgggtc gagcatcgga tgcgcgcgcc ccaccgtgaa
     1501 ctcgtcggcc cccaggtcga gcacccggtg gcgccccccg ccgccggtca ggttggaggc
     1561 gaccgggccg agcaggggct ccaggatcag cacgctctcg tgggcgagcg tgccgcccgc
     1621 gtagagaccg aggaccgccg actggccgga tgagagatcg cgccgcgcct ccgcgatcct
     1681 ccggctcacg tcgggcaccg agaacacgcc gggctgccag gccgtcccgg ccagcgcggc
     1741 gtcggcggcg agcgcggcgt cctcgagcgt cgcgaccgtc gtgaccgggc cctcgcggcc
     1801 cacgatcacg tcgcggccga gcaggcagac caccacgggc ttcccgatgc cggccagctt
     1861 cgcgcggatg cgctcgccga ccgccggcgc cggcggcttg ccgatcacca cgatcagctc
     1921 ggtggccgcg ttggccgcga gcaggtcgag cgcggccagg gtcatgatcc cgccgacggc
     1981 ctcgctcatg tcgcggccgc ccacgccgat ggcgtgggag aggccgcgcc cgcgcccggc
     2041 cagcagcgtg gccacctgct gcagcccggt gccggaggcg gccacgagac ccacgcaccc
     2101 gggcggtacc gcgttggcga agccgagcgg gacgtcggcc acgtacgcgg tgccacagtc
     2161 cgggcccatc aggaggagcc cgcgcgcggt ggcctggcgc ttgagcgcga tctcgtcggc
     2221 gaggctcacg ttgtcgctga agaggatgac gttgaggc
//
LOCUS       DATMCV010001060         4560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_325248, whole genome shotgun sequence.
ACCESSION   DATMCV010001060 DATMCV010000000
VERSION     DATMCV010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325248"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..588)
                     /locus_tag="VNU03_24320"
     CDS             complement(166..588)
                     /locus_tag="VNU03_24320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77672.1"
                     /translation="MNRNRTIGLCLTLLLTSLLIGGLAFDASAQQPTAPAPPAAAPSD
                     QKPAAPAPDSSAPSSSTSQSQPPAMQQAPSTNVQTETRTEKTERIVEREPGTFLGVNP
                     TIALMVGAALLIVIVFALVSMSRRKEEVRHTHHTHTGI"
     gene            complement(732..1193)
                     /locus_tag="VNU03_24325"
     CDS             complement(732..1193)
                     /locus_tag="VNU03_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWP77673.1"
                     /translation="MSGTTRVLALAVLALLLIVPSGFGAPDIDRSAVDFKTPAEIKWV
                     RNAAGTNEQAVLFGDPGKPGPYVVRLKWLPGNMSRPHFHPNDRFFVVISGTWWMGTGE
                     KFDPDATVPAPAGSYVIHYAGKVHYDGAKDEETVIQVWGMGPATSTPAEKR"
     gene            1246..2538
                     /gene="aroA"
                     /locus_tag="VNU03_24330"
     CDS             1246..2538
                     /gene="aroA"
                     /locus_tag="VNU03_24330"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWP77674.1"
                     /translation="MAETPAALLPIEPITRPFDAVVALPGSKSYSNRALLVAALARGT
                     SEITQALASDDTRYMHRALEALGVRVRADETARTFVVEGVDGRFPAAEATLQIGNAGT
                     AARFLTAAVSLGRGTFVVDGSPAMRKRPIEPLLEGLRALGVDAVSREGTGCPPVVVRA
                     AGIAGGRARMRGDISSQYFSALLLAAPYAQRDVEIEVEGDLVSAPYITMTLSTMEAFG
                     VRAERDGDRRFRVPAGQRYRARAYAVEPDASGASYFFAAAAVTGSRVVVPRLGRASAQ
                     GDLGLLDVLARMGCEVTVEADSVTVRGPAQLRAVDADFTRMGDVATTLMAIAPYADGP
                     VTVRGIAQTHFEESDRPVAAATELRRMGLQVESTHDSVTIHPGAPRPTDVQTYDDHRI
                     AMSFALTGLRAPGIRIVNPGCVSKTFPDYFDVLRGLSA"
     gene            2556..3110
                     /locus_tag="VNU03_24335"
     CDS             2556..3110
                     /locus_tag="VNU03_24335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77675.1"
                     /translation="MPRIAPITGKSDVPTEHHAVVDEVVRVFGNVRGPFSMLLHSPKL
                     AERLLPLVTFFRDESVVEGRLRSVAILAAVREREAAYVWSAQVAAARRNGVADQVIDL
                     LRAKGDPAGLPAEEREIVTYVRQLMRTNRVDRATFDALARRRGPQWLVEMTAAANYFA
                     MLSGVVNALEVEAPPDGDKLPASA"
     gene            complement(3112..4290)
                     /locus_tag="VNU03_24340"
     CDS             complement(3112..4290)
                     /locus_tag="VNU03_24340"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWP77676.1"
                     /translation="MTPLPQVDAVLRRAAESREVSGVVAMAATDRGVLYEGAFGRRVL
                     DHPEPMTLDSVFRIASMTKAITSVAAMQLVEQGKLALESPVPDIDPTLGSPQVLEGFD
                     ASGKPRLRPARRPITLRHLLTHTAGFGYRMWDPTVGRYLETAGLPARGGGHVASIRMP
                     LVFDPGERWEYGINTDWVGRLVEAASGRPLDAYFRDRIFAPLGMIDSGFVTTAEQRAR
                     QARLHQRQADGSLLLQPLEPPATAAEFWSGGGPLYSTGRDYLQLLKMLLHGGSWNGAR
                     LLRPETVALMGENHTGEIPAGVMKTALPEMSSDVDFFPGARIRWGLGYMLNLDPGPNG
                     RSAGTVSWGGLYNTYYWLDPARRVTGLIMTQILPFADPRVVALYGRFERAIYEALPAA
                     "
     gene            complement(4300..>4560)
                     /locus_tag="VNU03_24345"
     CDS             complement(4300..>4560)
                     /locus_tag="VNU03_24345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77677.1"
                     /translation="DGILVNNVCPGSIMSERMLSNVTARAKQLGIPLEEALAQRAAET
                     AVGRVGEPKELAHLVAFLASGKSSYITGTTILVDGGLVRSIL"
BASE COUNT          625 a         1606 c         1667 g          662 t
ORIGIN      
        1 cgcgtgcaca ccgccgagtc cggcgcttac cgcgccgcga tccaggtgtc ccacgtggcc
       61 gggcgccgct acgtggacaa gagctggcgc gtccagtgac ccatccgccg tgcgcgacct
      121 ccaggctacc ggaggccgcg cacggcgttc gattccgaag tcgggtcaga tgccggtgtg
      181 ggtgtgatgc gtgtgccgaa cttcctcctt ccgacggctc atcgacacga gggcaaacac
      241 gatcacgatc agcagcgccg cccccaccat caaggcgatc gtgggattga cgcccaggaa
      301 cgttccgggc tcacgctcga cgatgcgctc ggtcttctcg gtccgggtct cggtctgcac
      361 gttggtcgac ggggcctgct gcatcgccgg gggctgcgac tgcgatgtgc tgctgctggg
      421 tgcgctgctg tcgggtgcgg gcgccgccgg cttctggtcc gagggcgcgg ccgcgggcgg
      481 agccggcgcg gtcggctgct gcgcgctcgc atcgaaggcc aggcccccga tgagaagcga
      541 ggtgagaagc aaggtcaggc agagtccaat cgtccggttg cgattcatga gcgcgtcctc
      601 ctttgtcgcc agaggaggca atgggaatgc caccggcatt ccgcgcaaga tcagacactt
      661 gcgtcggtgt gcaacgccgg tgcggcactt tcacccgggt aggggttacc cggctcgtgc
      721 tggccggccc gctagcgctt ctcggcggga gtcgaggtgg ccggccccat gccccagacc
      781 tggatgacgg tctcctcgtc cttcgcgccg tcgtagtgca ccttgcccgc gtagtggatc
      841 acgtagctgc ccgccggcgc gggcacggtg gcgtccggat cgaacttctc gccggtgccc
      901 atccaccagg tgccggagat caccacgaag aagcggtcgt tcgggtggaa gtgcgggcgg
      961 ctcatgttgc cgggcagcca cttgaggcgg acgacgtacg gccccggctt gccgggatcg
     1021 ccgaagagca ccgcctgctc gttggtgccc gccgcgttcc gcacccactt gatctcggcc
     1081 ggcgtcttga aatcgaccgc gctccggtcg atgtcgggcg ctccgaagcc cgagggcacg
     1141 atcagcaaca gggccagcac cgcgagagcc agcacacggg tcgttccgct cataccgcct
     1201 ccttcggtga tatgggagct gaagtgaggg tatgatgcgc cagtcatggc cgagacacca
     1261 gccgcgctgc tgcccatcga gccgatcacg cgcccgttcg acgcggtggt cgcgctgccc
     1321 ggctcgaaga gctactcgaa ccgcgcgctg ctcgtggccg ccctggcccg cggcacctcc
     1381 gagatcaccc aggccctggc ctccgacgac acccgctaca tgcaccgcgc cctggaggcg
     1441 ctcggcgtgc gggtgcgggc cgacgagacg gcgcgcacct tcgtggtgga gggcgtggac
     1501 ggccgcttcc cggccgcgga ggccacgctc cagatcggca acgcgggcac cgcggcgcgc
     1561 ttcctgaccg cggcggtctc gctcgggcgg gggaccttcg tggtggacgg ctcgcccgcg
     1621 atgcgcaagc gcccgatcga gccgctgctc gagggcctgc gcgcgctcgg cgtcgacgcg
     1681 gtcagccgcg agggcaccgg ctgcccgccg gtggtggtgc gcgcggccgg gatcgcgggc
     1741 gggcgcgcgc gcatgcgtgg agacatctcg agccagtact tcagcgcgct cctgctcgcc
     1801 gcgccctacg cgcagcggga cgtcgagatc gaggtggagg gcgatctcgt ctcggcgccc
     1861 tacatcacga tgacgctctc caccatggag gccttcgggg tgcgcgcgga gcgcgacggc
     1921 gaccgacggt tccgggtgcc cgcggggcag cgctaccggg cgcgcgccta cgcggtcgag
     1981 ccggacgcct ccggcgcctc ctacttcttc gcggcggccg cggtcaccgg cagccgcgtg
     2041 gtcgtcccgc gcctgggccg cgcctccgcc cagggcgacc tcgggctgct cgacgtgctg
     2101 gcgcgcatgg gctgcgaggt gacggtggag gcggactcgg tcacggtgcg cggtcccgcg
     2161 cagctgcgcg cggtggacgc cgacttcacc cgcatgggcg acgtggcgac caccctcatg
     2221 gcgatcgcgc cctacgcgga cgggccggtg acggtgcggg ggatcgccca gacccacttc
     2281 gaggagagcg accggccggt ggccgcggcc accgagctgc ggcggatggg gctccaggtg
     2341 gagagcaccc acgactcggt caccattcat ccgggcgcgc cgcgccccac cgatgtgcag
     2401 acttacgacg accaccgcat cgcgatgagc ttcgccctga ccggcctgcg cgcgcccggc
     2461 atccgcatcg tcaacccggg ctgcgtgtcc aagaccttcc ccgactattt cgacgtcctg
     2521 cgcgggcttt ccgcctagcc cgggaggaga ccaccatgcc ccgtatcgcc ccgatcaccg
     2581 gcaaatccga cgtgcccacg gagcaccacg cggtggtgga cgaggtggtg cgcgtcttcg
     2641 gcaacgtgcg cgggcccttc agcatgctgc tgcacagccc gaagctggcc gagcggctgc
     2701 tgccgctggt caccttcttc cgcgacgaga gcgtggtgga ggggcgcctg cgctcggtgg
     2761 ccatcctggc cgcggtgcgc gagcgcgagg ccgcttacgt ctggtcggcc caggtcgcgg
     2821 cggcccggcg caacggggtg gcggaccagg tcatcgacct cctccgcgcc aagggcgacc
     2881 ccgccgggct gcccgccgag gagcgcgaga tcgtgaccta cgtgcggcag ctcatgcgga
     2941 cgaaccgggt ggatcgggcg actttcgacg cgctggccag gcgccggggg ccgcagtggc
     3001 tggtggagat gaccgcggcc gccaactact tcgcgatgct ctccggggtg gtcaacgcgc
     3061 tcgaggtgga ggcgcccccg gacggcgaca agctgcccgc gtcggcctag ctcacgcggc
     3121 cggtagcgcc tcgtagattg cgcgctcgaa gcgcccgtac agcgcgacca cgcgcgggtc
     3181 ggcgaagggc aggatctggg tcatgatgag cccggtgacg cgccgggccg gatcgagcca
     3241 gtaatacgtg ttgtagagcc cgccccagct cacggtgccc gcgctgcggc cgttcggtcc
     3301 cgggtccagg ttgagcatgt agccgagccc ccagcggatc cgcgcccccg ggaagaagtc
     3361 cacgtcgctc gacatctcgg gcagggcggt cttcatgacg ccggccggga tctcgccggt
     3421 gtggttctcg cccatcaggg ccaccgtctc cgggcggagc aggcgcgccc cgttccagct
     3481 cccgccgtga agcagcatct tgaggagctg gagatagtcg cgcccggtcg agtagagcgg
     3541 cccgccgccc gaccagaact ccgccgcggt cgcgggcggc tcgagcggct ggagcaggag
     3601 cgagccgtcg gcctgccgct ggtgcagccg cgcctggcgc gcccgctgct cggcggtggt
     3661 cacgaagccg ctatcgatca tgccgagcgg cgcgaagatg cggtcgcgga agtaggcgtc
     3721 gagcggtcgc ccgctggcgg cctccacgag ccggcccacc cagtccgtgt tgatgccgta
     3781 ctcccaccgc tcgcccggat cgaagacgag cggcatccgg atggacgcca cgtgcccgcc
     3841 gccccgcgcg ggcagccccg cggtctcgag atagcggccg acggtcggat cccacatgcg
     3901 gtagccgaag ccggcggtgt gggtgagcag gtgacgcagg gtgatcggcc gccgcgcggg
     3961 gcggaggcgc ggcttgcccg atgcgtcgaa gccttccagg acctggggcg agccgagcgt
     4021 cggatcgatg tcgggcaccg gcgactcgag cgcgagcttg ccctgctcca ccagctgcat
     4081 cgcggcgacc gaggtgatgg ccttggtcat cgaggcgatg cggaagacgc tgtcgagcgt
     4141 catcggctcg gggtggtcga gcacgcgccg gccgaacgcg ccctcgtaga ggacgccgcg
     4201 gtcggtagcg gccatcgcca ccacgcccga cacctcgcgg gattcggcgg cccggcggag
     4261 gacggcgtcg acctgcggca gcggagtcac gcgctccgcc tagaggatgg agcgaacgag
     4321 gccgccgtcg acgaggatcg tggtcccggt gatgtagctc gacttaccgg aggcgaggaa
     4381 cgccaccagg tgggccagct ctttcggctc gcccacgcgg cccaccgcgg tctcggccgc
     4441 gcgctgggct agagcctcct cgagcgggat gccgagctgc ttggcgcgcg cggtcacgtt
     4501 ggacagcatg cgctcgctca tgatcgagcc ggggcacacg ttgttgacca ggattccgtc
//
LOCUS       DATMCV010001061         3975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_192912, whole genome shotgun sequence.
ACCESSION   DATMCV010001061 DATMCV010000000
VERSION     DATMCV010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192912"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..583)
                     /locus_tag="VNU03_24350"
     CDS             complement(80..583)
                     /locus_tag="VNU03_24350"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HWP77678.1"
                     /translation="MADLTYRSPRTEVRPSPIQGQGLFARVPIAAGEIVAVKGGHVLT
                     GSQWSALEPTLGAAEIQIAEDLFIAPVREGHRAGSMLYTNHSCDPNLAIQGQIVLVAM
                     RDVAADEELTIDWATTDDGDHRMTCHCGSPRCRGIVTGKDWMKPELQQRYRGWFCWFL
                     QRKIDAA"
     gene            complement(705..2363)
                     /locus_tag="VNU03_24355"
                     /pseudo
     CDS             complement(705..2363)
                     /locus_tag="VNU03_24355"
                     /inference="COORDINATES: protein motif:HMM:NF004807.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
     gene            2434..3528
                     /locus_tag="VNU03_24360"
     CDS             2434..3528
                     /locus_tag="VNU03_24360"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWP77679.1"
                     /translation="MARKKHTVGIIGLGFGRAHIPAFQVSGCEVVALCQRNQETARAM
                     AQRYNVPAVFERWEEMLDQAKPDIVVIASPPHLHREIALRALGQGAHVLCEKPLAMTA
                     AEGREMVEAAARARRVAMTGFNWRFIPAMQRFHALVEEGAVGRLFHAGGRWLGARWAD
                     EAAPITWRMDRTQAGHGAMGDMGVHLIDLVRWHFGEFTRVAAQAGVAYASRSVPGGGK
                     PADAEDFCTVLGELASGGQVTLSVSRAARGANEASLEAYGSQGALVYRLDRDKPKWYV
                     GELRGAGASGTLQPVPVKAGLPRSAGEGDPIEVTGKATIAPLVKRFLEGIRKGESPSP
                     SLEDGLKAQLVLDAVLRSLTEGGWQRVEPA"
     gene            3525..>3975
                     /locus_tag="VNU03_24365"
     CDS             3525..>3975
                     /locus_tag="VNU03_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HWP77680.1"
                     /translation="MTPARPGLRVGVIGEGVCSRRVAALAERVGRAIAEAGAVLLCGG
                     LGGVMEAAARGAARAGGLVVGVLPGFEARDANRWVGVPIVTGMDQARNVILVRSSDAV
                     IAVGGRYGTLSEIALALKLGRPVVGLGTWRLAQPEGRRVPLPSARTPE"
BASE COUNT          535 a         1324 c         1478 g          638 t
ORIGIN      
        1 atgtagtcca tgtactgagc gaaggccatg cccttcgcaa agcgctccgg cgtgacgacg
       61 cgatgtccgg ggtcgaggct catgctgcgt cgatcttccg ctgcaggaac cagcagaacc
      121 acccgcggta ccgctgctgc agctccggct tcatccagtc cttgccggtg acgatgccgc
      181 ggcagcgcgg gctgccgcaa tggcacgtca tccgatggtc gccgtcgtcg gtggtggccc
      241 agtcgatcgt gagctcctcg tcggcggcga cgtcccgcat cgcgaccagc acgatctggc
      301 cctggatggc caggttgggg tcgcaggagt ggttggtgta gagcatgctg ccggctcggt
      361 ggccctcacg caccggggcg atgaagagat cctccgcgat ctggatctcg gcggcgccga
      421 gcgtgggctc gagagcggac cactgcgatc cggtgagcac gtgcccgccc ttgaccgcga
      481 cgatctcgcc cgccgcgatg gggactcgcg cgaagaggcc ctggccctgg atcgggctgg
      541 gccgcacctc ggtccggggc gagcgatacg tgagatcagc catgggcgga cacgggggac
      601 agcggggcca ggcgataggt catgacggct ccgagctcag gtccccttgg ggccctcacc
      661 ctgccctctc cccggagggg agagggtggg agggagaggc gcgcttactt gaagatcgag
      721 aagttgatct ctttctcggt gatgaactcg agcaggaccg ggacgccctc gcgggtcttg
      781 gcgatgccgc gcttgatcgc gttgacgatc tcgcccggct cggtgacgcg ctccccgtag
      841 ccgccgaagg ccttcgccat gtccgcgtag tggccgctga tgtcggtgga gcggtacttc
      901 tcggtggcgg ccttcatgat cggcagctcg atggccatcg agaagttgtt gagcaggatc
      961 gacaggatcg ggatgcgctc gcgcaccgcg gtctcgaagt ccatgccggt gaaaccgatg
     1021 gccgcgtcgc cccacacgtt gatgcagagc gcttccggct tcgcgagctt ggcccccatc
     1081 gcgaggccga ggccgtagcc gagctgggtg gtcttgcccc agccgatgta ggtgagcggg
     1141 tgcgtggatt cccagaaggg ggacagctga tccctcgggc tccccgcgtc gtgggtgatg
     1201 atggtgttgg ccacgtccac cgtgtgcagc aggtcccaga tcacgcggta gggcgagagc
     1261 ggcttgtcgt tggcggtcag cttgggcatc cactgggcca tccactccgc cttcatggtg
     1321 gcgatctgcc gcgtgacctc cgccttgcgg ccgcgcggct tgctcttcag ccggtcgcgc
     1381 acctcggcga gcagggcgtc cagcaccaga cccgcgtccc cgatgaccgc gtgctcggcc
     1441 acgatgtcct tgttcagatc ggccacatcg atggtggagt ggatgatggt cttgcccttc
     1501 ggcatcgtga cgccgtagtt ggtggtcgag aagctgcagc cgatgccgaa gatcacgtcc
     1561 gagttcttca ggaactcgtg gaccgtcttc ggcatcgagc ggccgcccga gccgagcgac
     1621 agggggtggt tctccgggaa cgcgctcttg ccctcgaggc tggtggtgac cggcgcctcc
     1681 agcagctcag ccagctcgcg cagctgctgc cacgcacgcg cgtagtgcac gccctggccc
     1741 gcgtagatca ccgggcgctc cgcctcgacc aggatgcgcg cggcctcggt caccgcatcg
     1801 ggatccgggg ccacccgcgt gcgcggggtc gccttgtagt tgagcggctc cggcacctct
     1861 tcctgataga cgtcgaccgg gatctccacc agcaccggcc gcggccggcc gttcttcacc
     1921 tgggtgaagg cgcggcgcat cgcgtccggc accgcgtcgc ccaccacgac ctgctcgcac
     1981 cacttggtca cgttgcggta gttgatgaac gagttgaagt tcggcgggat gttggtgatg
     2041 cggcgcggat agccgccggg caacaccacg atcggcaccg agtcgccgta ggcctgggct
     2101 accccgccga aggcgttctc cgagccgggg ccgtgctgca tcgtgaacac gccgatcttc
     2161 tggccggagg tgatgcggct caccgcgtcg gccatgtgca ggccggtgcg ctcctgccgg
     2221 acgatgatgg ttcggatgtc ggcctgggcg gccgcctcga tgatcgggtt gaccgggtag
     2281 ccgatcagaa aggtggtacc ttcgcgcttc aggatctcgg cgactgcggc gaccgccttc
     2341 atggggccct cctggggatc tgctgtagga tggggccgac tctaacccaa tccgcggatg
     2401 aagggaatcc gccggcgaaa ggatcgacga ggcatggctc ggaagaagca cacggtgggc
     2461 atcatcgggc tcgggttcgg ccgcgcgcac atccccgcgt tccaggtgag cgggtgcgag
     2521 gtggtggcgc tctgccagcg caaccaggag accgcgcggg cgatggccca gcgctacaac
     2581 gtgcccgccg tcttcgagcg ctgggaggag atgctcgacc aggccaagcc cgacatcgtg
     2641 gtgatcgcct cgccgccgca cctccatcgc gagatcgcgc tccgcgcgct cggccagggc
     2701 gcgcacgtgc tctgcgagaa gccgctcgcc atgaccgcgg ccgagggccg cgagatggtg
     2761 gaggcggcgg cgcgcgcccg gcgggtggcg atgaccggct tcaactggcg cttcatcccc
     2821 gcgatgcagc gcttccacgc gctggtggag gagggcgcgg tgggccggct cttccacgcg
     2881 ggcggccgct ggctgggcgc gcgctgggcc gacgaggcgg cgccgatcac gtggcgtatg
     2941 gatcgcaccc aggccggcca cggggcgatg ggcgacatgg gcgtccatct gatcgatctc
     3001 gtgcgctggc acttcggcga gttcacccgg gtggccgcgc aggccggcgt cgcctatgcg
     3061 tcgcgcagcg tcccgggggg cggcaagccg gcggacgcgg aggacttctg cacggtgctg
     3121 ggcgagctgg cctcgggcgg gcaggtgacg ctctcggtga gccgggccgc ccgcggcgcc
     3181 aacgaggcct ctctcgaggc ctacggcagc cagggcgcgc tcgtctaccg gctcgaccgc
     3241 gacaagccca agtggtacgt cggcgagctg cgcggcgccg gtgcgagcgg cacgctccag
     3301 ccggtcccgg tcaaggccgg gctgccccgc tcggccggcg agggcgatcc gatcgaggtc
     3361 accggcaagg ccacgatcgc cccgctggtg aagcgcttcc tcgagggcat ccgcaagggg
     3421 gagagcccgt cgccctcgct cgaggacggc ctgaaggccc agctcgtgct ggacgcggtg
     3481 ctgcgctccc tcaccgaagg gggctggcag cgcgtcgagc ccgcgtgacg ccggcgcgcc
     3541 ccggcctccg ggtgggcgtc atcggcgagg gcgtgtgctc gcgccgggtg gccgcgctcg
     3601 cggagcgcgt cggccgcgcc atcgcggagg ccggcgcggt gctcctttgc ggtggcctcg
     3661 gcggcgtgat ggaggcggcc gcgcgggggg ccgcccgggc cggcgggctg gtggtcggcg
     3721 tcctgcctgg cttcgaagcc cgtgacgcca atcgctgggt cggcgtgccg atcgtgaccg
     3781 gcatggacca ggcgcgtaac gtgatcctgg tgcgctcgtc ggatgccgtc atcgcggtcg
     3841 gcgggcgtta cggcaccctg tccgagatcg cgctcgcgct gaagctgggg cggccggtgg
     3901 tggggctcgg tacctggcgc ctggcccagc cggaaggccg ccgggttccg ttgccgagcg
     3961 cccggacccc ggagg
//
LOCUS       DATMCV010001062         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_723720, whole genome shotgun sequence.
ACCESSION   DATMCV010001062 DATMCV010000000
VERSION     DATMCV010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723720"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1531
                     /locus_tag="VNU03_24370"
     CDS             77..1531
                     /locus_tag="VNU03_24370"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP77681.1"
                     /translation="MSVEIPASGSSVPAVTEPAAVPPEHAAIRVPLKVKLSLLITALV
                     VLAVALVGLFLLRQQQQSLTAEMTKRGLTMAENFAAGAKTPLLTGDDLTLGVLVTDAM
                     KDPDVSYVIVADHDGRVLAHSDPQAAVKSAVDRPKGLEPLKDRLLVQIYKTPQGRVID
                     FAVPLVYSGVPVGALYLGFSEEAITAALASARNQALLITLLMVLAGLGGAVGLAALLS
                     RPIFRLVQATGAIAAGNFNIALPVASRDELGVLTDSFNRMARSLREKEMIKRAFTRYV
                     AREVVEEILKNPENMALTGERREVTVLFCDVRGFTPLSERLSPEEVVLLLNDFYNLMI
                     ETTFKHDGTLDKFLGDAVMAVFGAPLAHPDHSARAIRTALAMQEGIVGLNERRARDGK
                     EPIAVGIGVSAGEAVAGTVGTEDRMEYTVIGDSVNLAARLESNAKPGQILISHRTYER
                     VRDLVDARPLGRIRVKGKEEEVEVYEVLRLAPVV"
     gene            1566..>2095
                     /locus_tag="VNU03_24375"
     CDS             1566..>2095
                     /locus_tag="VNU03_24375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77682.1"
                     /translation="MITRRARRRALVVGGLALLLGACATAAPAPAPGTASTPARPAPR
                     PAAYESEDFVVTFAQAGDTPATLAARYLGSADRAWMIEDYSGQQAFSPGQEVVIPRRP
                     WNPAGVTPGGYQIVPILTYHNLGEQAKGRLILAAASFREQMQYLKTNGYRVVSLADFI
                     EFTRLGRQLPQRAVVLT"
BASE COUNT          310 a          756 c          732 g          297 t
ORIGIN      
        1 ggccgaggag tacgcgcgcc gccaggtggg cgagatccag gaccggatct tccccctcca
       61 tggactgtag gcccgcgtga gcgtggagat ccccgcgagc gggtcgagcg tcccggcggt
      121 gaccgagccg gcggcggtgc cccccgagca cgccgcgatc cgcgtgccgc tcaaggtcaa
      181 gctgagcctg ctcatcaccg cgctggtggt gctggcggtg gccctggtcg gcctcttcct
      241 cctgcgccag cagcagcaga gcctcaccgc cgagatgacc aagcgcggcc tcaccatggc
      301 cgagaacttc gcggccgggg ccaagacccc gctgctcacc ggcgacgacc tgaccctggg
      361 cgtgctggtg accgacgcca tgaaggaccc cgacgtctcc tacgtcatcg tggccgacca
      421 cgacgggagg gtcctcgcgc actccgaccc gcaggccgcg gtgaagagcg cggtcgaccg
      481 gcccaagggg ctcgagccgc tcaaggaccg gctgctggtc cagatctaca agaccccgca
      541 gggccgggtc atcgacttcg cggtgcccct ggtctacagc ggggtgccgg tgggcgcgct
      601 ctacctgggc ttcagcgagg aggcgatcac cgcggcgctg gccagcgcgc gcaaccaggc
      661 actcttgatc actttgctga tggtcctggc cggcctcggc ggggcggtgg gcctggccgc
      721 cctgctctcg cggccgatct tccgtctcgt gcaggccacc ggggccatcg cggcgggcaa
      781 cttcaacatc gcgctgccgg tggcctcccg cgacgagctc ggggtgctga ccgactcctt
      841 caatcgcatg gcccgcagcc tgcgcgagaa ggagatgatc aagcgcgcgt tcacccgcta
      901 cgtggcgcgg gaggtggtcg aggagatctt gaagaacccg gagaacatgg cgctcaccgg
      961 ggagcggcgg gaggtcacgg tgctcttctg cgacgtgcgc gggttcacgc cgctctccga
     1021 gcgcctgagc ccggaagagg tcgtgctcct gctcaacgac ttctacaacc tgatgatcga
     1081 gaccaccttc aagcacgacg gcaccctcga caagttcctg ggcgacgcgg tgatggcggt
     1141 gttcggcgcc cccctcgccc atcccgatca ctccgcgcgg gccatccgca ccgcgctcgc
     1201 catgcaggag gggatcgtcg ggctgaacga gcggcgcgcg cgcgacggca aggagccgat
     1261 cgcggtcggc atcggggtga gcgcgggcga ggcggtggcg ggcacggtgg gcaccgagga
     1321 ccggatggag tacaccgtga tcggcgacag cgtgaacctg gccgcgcgcc tcgagtccaa
     1381 cgccaagccg ggtcagatcc tgatcagcca ccgcacctac gagcgcgtcc gcgacctggt
     1441 ggacgcgcgg cccctggggc gcatccgcgt caagggcaag gaagaggaag tcgaggtcta
     1501 cgaggtgctg cggctggccc cggtagtttg attccgttct tcgcccggtc gcgtaggata
     1561 tggggatgat cactcgacgc gcgcggagac gggccctcgt ggtgggcggg ctggcgctcc
     1621 tgctgggcgc ctgcgccacc gcggcgcccg cgcccgcccc gggcaccgcc tccaccccgg
     1681 cccgtcccgc cccccgtccg gccgcctacg agtccgagga cttcgtcgtc acgttcgccc
     1741 aggcaggcga tacccccgcg acactcgcgg cgcgctacct cggctccgcc gaccgcgcgt
     1801 ggatgatcga ggactacagc gggcagcaag ccttctcccc cggccaggag gtggtcatcc
     1861 cccggcggcc gtggaacccg gccggggtca cgccgggcgg ctaccagatc gtgccgatcc
     1921 tcacctacca caacctgggg gagcaggcga agggccgcct catcctggcc gcggcgtcct
     1981 tccgcgagca gatgcagtac ttgaagacca acggctaccg ggtcgtgagc ctcgccgact
     2041 tcatcgagtt cacccggctc ggccgccagc tcccccagcg ggcggtggtc ctgac
//
LOCUS       DATMCV010001063         2621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_594793, whole genome shotgun sequence.
ACCESSION   DATMCV010001063 DATMCV010000000
VERSION     DATMCV010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594793"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VNU03_24380"
     CDS             complement(<1..213)
                     /locus_tag="VNU03_24380"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77683.1"
                     /translation="MTVARHSQTFPARRDALPRVDAFLGEVCSGAGLGRDTCLRLTLL
                     VEELFTNTVVHGHGGDSEAPVRIECEV"
     gene            251..1282
                     /locus_tag="VNU03_24385"
     CDS             251..1282
                     /locus_tag="VNU03_24385"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP77684.1"
                     /translation="MGTARARIHRRQLAGVMLAAAAITPFFNLLTSRASVAEAVQGFV
                     DAVLVSLLTGGYLIFVRDGRARAWFRRIGFLTDLALSSVIVLVLFLVGRAAGQVVTSF
                     NPHRFLMSFTDAHLIYAIPFFVIVGVTVQFILQMNRLIGGNVLGYFVTGVYTRPKAEE
                     RIFLFMDLAGSTQLAERLGSARYFELLRRVVDDLTEPVLQTKGEIYQYAGDEVVITWP
                     TEKGLRAANCVRCFFLICAAVSGNPARYEREFGARPTFRGGLHGGTVTAGELGDLRQQ
                     IVFVGDILNTAARLEEYAKQAGFDLVASGAVLDRLTLPTGVKATPCGELALRGKAASV
                     AAYGLTNGG"
     gene            complement(1279..1800)
                     /locus_tag="VNU03_24390"
     CDS             complement(1279..1800)
                     /locus_tag="VNU03_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWP77685.1"
                     /translation="MVRSMMTGRRAFLRVGVLGGGASLLAMALPVRLARAAATDALLL
                     SCMDYRLTNETERYMSGRGLRNKYDHVILAGASLGALTDKYPAWNQTFWEHLEVAIQL
                     HQIHKVILLDHRDCGAYQVILGEDFAKDRAKETTVHAAQLDELRKRIGAQYPTLEVET
                     LLMSLDGKVEKVG"
     gene            complement(1906..2376)
                     /locus_tag="VNU03_24395"
     CDS             complement(1906..2376)
                     /locus_tag="VNU03_24395"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP77686.1"
                     /translation="MRVTVRSAGIDEAHAVQRIMQVAFEEYRGRLDPPSGALSETIDD
                     VRAAISRGGAFLALSSDMAVGSARFRLSPGYVYAGRVAVLPAYRGKGVAGALMAAIEA
                     SARAHGVAEVRVGVRGSLPANRRLYEKLGYRVLSSRPYETGTDVDITLSKELEP"
     gene            complement(2385..>2621)
                     /locus_tag="VNU03_24400"
     CDS             complement(2385..>2621)
                     /locus_tag="VNU03_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HWP77687.1"
                     /translation="LQIQDEAVIAVSGKAQHTMARFTGRPSDVIPGTVFAVSPEEIQR
                     ADEYEVAAVKRVAVVLQSGARAWVYVDARYTPSS"
BASE COUNT          381 a          952 c          853 g          435 t
ORIGIN      
        1 cacctcgcac tcgatacgca ccggcgcctc gctgtccccc ccgtggccgt gcaccacggt
       61 gttggtgaag agctcctcca ccaggagagt gagccgcaga caggtgtcgc gcccgagccc
      121 ggccccggag cagacctcgc cgaggaaggc gtccacccgt ggcaaggcgt cgcggcgggc
      181 ggggaacgtc tgactgtggc gcgcaaccgt catcggccta ttgtacggcc ggcggtggta
      241 tatccgaggc atgggaacgg cccgcgcgcg catccaccgt cgtcagctcg cgggggtcat
      301 gctcgcggcc gccgcgatca cgccgttctt caacctgctg accagccggg cctccgtggc
      361 cgaggccgtc cagggcttcg tcgacgcggt gctggtgtcg ctcctcaccg gcggctacct
      421 catcttcgtc cgggacgggc gcgctcgcgc gtggttccga cgcatcggct tcctcaccga
      481 tctcgcgctc agcagcgtga tcgtgctggt gctgttcctc gtcggccgcg ccgcgggtca
      541 ggtggtgacc agcttcaatc cacaccggtt cctgatgagc ttcaccgacg cccacctgat
      601 ctacgccatc cccttcttcg tgatcgtcgg ggtcaccgtg cagttcatcc tccagatgaa
      661 ccggctgatc ggcggcaacg tgctggggta cttcgtcacc ggcgtctaca cccggcccaa
      721 ggcggaggag cgcatcttcc tgttcatgga cctggccggc tcgacccagc tcgcggagcg
      781 cctcgggagc gcccgctact tcgagctact gcgccgcgtg gtggacgacc tgaccgagcc
      841 ggtgctgcag accaagggag agatctacca gtacgcgggc gacgaggtcg tcatcacctg
      901 gcccaccgag aagggcctgc gggccgccaa ctgcgtccgc tgcttcttcc tgatctgtgc
      961 cgcggtctcg ggcaaccccg cccggtacga gcgcgagttc ggggcgcgcc ccacttttcg
     1021 cggcggcctc cacggcggca ccgtcaccgc cggggagctg ggcgacctgc ggcagcagat
     1081 cgtcttcgtg ggagacatcc tgaacaccgc cgcgcgcctc gaggagtacg ccaagcaggc
     1141 gggcttcgat ctcgtcgcct cgggagcggt gctcgaccgg ctcaccctgc cgacgggcgt
     1201 gaaggcgacg ccgtgcgggg agctggccct gcggggcaag gccgcgagcg tggcggccta
     1261 cggcctgacg aacggcggct agcccacctt ctcgaccttg ccgtcgaggc tcatcaggag
     1321 cgtctccact tccagggtgg gatactgggc gccgattcgc ttgcgcagct cgtcgagctg
     1381 cgcggcgtgg accgtggtct ccttcgcgcg gtccttggcg aagtcctcgc ccaggatcac
     1441 ctggtaggcg ccgcagtccc gatggtccag caggatcacc ttgtggatct ggtgcagctg
     1501 gatggccacc tccaggtgct cccagaacgt ctggttccag gccgggtact tgtcggtgag
     1561 cgctccgagg gaagcaccgg ccaggatgac gtggtcatac ttgttccgca ggccccgtcc
     1621 tgacatatac cgctcggtct cattggtcag ccgatagtcc atgcagctca gcaggagggc
     1681 gtcggtcgcg gccgctcgag ccaagcggac gggcagggcc atcgcgagga ggctcgcgcc
     1741 accgccgagc accccgaccc gcaggaacgc gcggcgaccg gtcatcatgc ttcgcaccat
     1801 tgctcgagca tcatctgcgt ggcctctcag gcgaagttgt tcactggttc ccagcgggcg
     1861 ccgggattct ctcgcttcgc gatcgcgaac acaagacgtc aagcactacg gctccagctc
     1921 cttgctcagc gtgatgtcca cgtcggtgcc cgtctcgtac gggcgcgagg acaggacccg
     1981 gtaaccgagc ttctcgtaca gccgacgatt cgcgggcagg ctcccccgga cgcccacccg
     2041 cacttccgcc acgccgtgag cgcgggcgct cgcctcgatc gccgccatca gggcgccggc
     2101 gactcccttc ccgcggtacg cgggcagcac cgcgaccctc ccggcgtaga cgtagcccgg
     2161 ggacaggcgg aaccgcgcac tcccgacggc catgtcactg gacaaggcga gaaaggcgcc
     2221 gccgcggctg atcgcggcgc gcacgtcgtc gatcgtttcg ctcagcgccc cgctcggcgg
     2281 gtcgagccgg ccgcggtact cctcgaacgc gacctgcatg atgcgctgga ctgcgtgcgc
     2341 ctcgtcgatc cccgcgcttc gaaccgtaac ccgcatgccg cccgtcagct cgacggcgtg
     2401 tagcgcgcat cgacatacac ccacgcgcgt gcccccgact ggagaaccac cgccacccgc
     2461 ttgaccgcgg ccacctcgta ctcgtccgcc ctctggatct cctccggcga gaccgcgaac
     2521 accgttcccg gtatgacgtc ggacgggcgt ccggtgaatc tggccatggt atgttgcgcc
     2581 ttgccgctca cggcaatcac cgcctcgtcc tggatctgca g
//
LOCUS       DATMCV010001064         3468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_791024, whole genome shotgun sequence.
ACCESSION   DATMCV010001064 DATMCV010000000
VERSION     DATMCV010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791024"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..488)
                     /locus_tag="VNU03_24405"
     CDS             complement(75..488)
                     /locus_tag="VNU03_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77688.1"
                     /translation="MRAFCAWCQRDGGPSFLGEREPFDDDSETHGVCRRHLQYILAGL
                     PSHSFPDVTYLVVVRRGEDGLHDYLDRAFLGVRGVKVIMDRRERERRGSERRASDERR
                     QVERRQPRGISYALGYTTVRFGAASEDVRAEAALG"
     gene            607..1173
                     /locus_tag="VNU03_24410"
     CDS             607..1173
                     /locus_tag="VNU03_24410"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWP77689.1"
                     /translation="MTRIGKLTAALVALLASTAFAQTPATSPGTVTGTVQNGSNVSIE
                     YTLKDEGGQVLDTNKGKNPLTFTQGQQQIIPGLERELVGMHAGDEKKVVVKPEDAYGP
                     IVPNAQTEVPKDSVPKEGLKVGTPLMARSGSGETRPVVVKEIKENTVVLDLNHPLAGK
                     TLFFDVKVVGVDAPAPGAPAAPAAKPTK"
     gene            complement(1261..2127)
                     /gene="dapF"
                     /locus_tag="VNU03_24415"
     CDS             complement(1261..2127)
                     /gene="dapF"
                     /locus_tag="VNU03_24415"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWP77690.1"
                     /translation="MIPFAKGHGLGNDYIVIDAADLPAALTAPQITRICDRNWGVGSD
                     GILLLTPPWDGADFGLRILNPDGSEAEKSGNGLRIFAKYLHDHGHAKKPTFTVATKGG
                     RVECTCHVVDGRMNLVAVEMGRCTFVAPEIPMNGPAREVVKVPLQVDGETLLITAVSV
                     GNPHCVIFTDRLDEALVRRLGPKVEHHPSFPNRINVQWARVASRSMVDILIWERGAGW
                     TLASGSSSSAVACAAVRNGLCDHGLVTVRMPGGELSIDVRPDWSIRLKGAVEEVYTGA
                     LTSDFMATLTQV"
     gene            complement(2124..2942)
                     /locus_tag="VNU03_24420"
     CDS             complement(2124..2942)
                     /locus_tag="VNU03_24420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWP77691.1"
                     /translation="MATSAGTTSVVLVVYNQLGLTRACLESLRSTTRPFELCVVDNAS
                     SDGSEAFFREWAAGTPLRYHRNADNVGLIRALNQGAGLAGGDRLCFLHNDTVMREPRW
                     LERLEAAVDGPGRVGLAGLYGVRRLRRDGRYVGRTIVHALEGQPTVRAPVTEVAAVDG
                     VCLFLPRTILDAVGGFDEAYGFFHGYDRDLSFAVREAGWRCVVVDAPFVHRGGGTRTG
                     EGAPVAAPEDLAQRRAALARFAGKWRHRLPCDVRGLPARLRDWLAAPRAVSSPA"
     gene            complement(2989..>3468)
                     /locus_tag="VNU03_24425"
     CDS             complement(2989..>3468)
                     /locus_tag="VNU03_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWP77692.1"
                     /translation="LKGPLHGGANEQVMLMVEKIKDPAKADAWIHKALTDKARIMGFG
                     HRVYRVEDPRAKHLKRLALELGQQVGDTSKVQILDTVVRVMGAEKHIFPNVDLYSGAA
                     YAAMGISTDQFTPIFAMSRVAGWTAHVLEQHGNNRLIRPRSDYTGAIDLKYVPLAER"
BASE COUNT          509 a         1271 c         1210 g          478 t
ORIGIN      
        1 ccccggcctg ctcaccgacg aggcggcgcg cgccccccag accccgcacc ggtcctaggg
       61 cgccccggcc gggctcaccc gagtgccgct tctgccctca cgtcctcgga ggccgcgccg
      121 aagcgtaccg tggtgtagcc gagggcatag gagatcccgc gcggctgccg gcgctccacc
      181 tggcgccgtt cgtcactcgc ccgacgctcg gagcctcggc gctcccgctc gcgccggtcc
      241 atgatcacct tcaccccgcg cacgcccagg aaggcccgat ccaggtagtc gtgcaggccg
      301 tcctcgccgc ggcggaccac gaccaggtag gtgacgtccg ggaaggagtg cgacggcagg
      361 ccggccagga tgtactggag gtgacgccgg cagaccccgt gcgtctcgct atcatcgtcg
      421 aacggctcgc gctccccgag aaaggagggt ccgccgtctc gctgacacca ggcgcagaag
      481 gctctcaagg tcgggcgatg atagcacggt ccgcggcgcg gccagtcgcg ttgcgtcgcc
      541 ccggagggcg ggtgtagagt cgaatcgcgc gcctgttgct tctctcgtgc gcgcctggag
      601 gtgacgatga ctcgcatcgg gaagctcacc gccgctctcg tggccctgct cgcgtccacc
      661 gccttcgcac agaccccggc cacgtcgccg ggcaccgtca ccggcacggt gcagaacggc
      721 tccaacgtga gcatcgagta caccctgaag gacgagggcg gccaggtgct cgacaccaac
      781 aagggcaaga acccgctcac cttcacccag ggccagcagc agatcattcc cggcctcgag
      841 cgggagctgg tgggcatgca cgcgggcgac gagaagaagg tcgtggtcaa gcccgaggac
      901 gcctacggcc cgatcgtgcc caacgcgcag accgaggtgc ccaaggactc ggtgcccaag
      961 gaagggctca aggtcggcac tcccctgatg gcccgcagcg gctcgggcga gacgcgtccg
     1021 gtggtggtga aggagatcaa ggagaacacg gtcgtgctcg acctgaacca cccgctcgcg
     1081 ggcaagaccc tcttcttcga cgtcaaggtc gtcggcgtcg acgcgcctgc tccgggcgcc
     1141 ccggccgcgc ccgccgccaa gccgaccaag tagcgccgcg cagatccggg cctgtgggcc
     1201 ccgcgggtcc ggtcgacgtt cgaccgcgcc cgccgtggcc cgcaggcctc ccgaccgcgg
     1261 tcagacctgg gtcagggtgg ccatgaagtc ggaggtgagc gcgccggtgt agacctcctc
     1321 gaccgcaccc ttgagccgga tcgaccagtc ggggcgcacg tcgatcgaca gctccccgcc
     1381 cggcatccgc accgtgacga gcccgtgatc gcagaggccg ttgcggaccg cggcgcacgc
     1441 caccgcgctc gaggacgatc cggaggcgag cgtccatccg gccccgcgct cccagatgag
     1501 gatgtcgacc atggagcgcg acgccacccg cgcccactgc acgttgatgc ggttcgggaa
     1561 cgagggatgg tgctcgacct tgggcccgag ccgccgcacc agcgcctcgt cgaggcggtc
     1621 ggtgaagatc acgcagtggg gattcccgac cgacaccgcg gtgatcagca gggtctcccc
     1681 gtccacctgc agcggcacct tcaccacctc gcgcgcgggc ccgttcatcg ggatctccgg
     1741 ggcgacgaag gtgcagcgcc ccatctccac cgcgaccagg ttcatgcggc catccaccac
     1801 gtggcaggtg cactcgaccc gtccgccctt ggtggccact gtgaaggtgg gcttcttggc
     1861 gtgcccgtgg tcgtggaggt acttggcgaa gatgcggagc ccgttgcccg acttctcggc
     1921 ctcgctgccg tcggggttca ggatgcgcag cccgaagtcg gctccgtccc acggcggggt
     1981 caggagcagg atgccgtccg agcccacgcc ccagttgcgg tcgcagatgc gggtgatctg
     2041 cggcgcggtg agggcggccg ggagatccgc cgcgtcgatc acgatgtagt cgttgccgag
     2101 gccgtggccc ttcgcgaatg ggatcatgcg ggtgatgata ccgcccgcgg cgccgcgagc
     2161 caatcgcgca ggcgcgcggg cagcccgcgg acgtcgcacg gcagccggtg gcgccacttc
     2221 ccggcgaagc gcgcgagcgc ggcccggcgc tgggccaggt cctccggggc ggccaccggc
     2281 gcgccctcgc cggtgcgcgt gccgccgccc cgatggacga acggcgcgtc caccaccacg
     2341 cagcgccacc ccgcctcgcg caccgcgaag gagagatccc ggtcgtagcc gtggaagaag
     2401 ccgtacgctt cgtcgaaccc gcccaccgcg tcgaggatcg tccgcggcag gaagaggcac
     2461 accccgtcca ccgccgcgac ctcggtcacc ggcgcgcgca ccgtcggctg cccctcgagc
     2521 gcgtggacga tggtccgccc cacgtaccgg ccgtcgcgac gcagccgccg caccccgtag
     2581 aggccggcca ggccgacgcg cccgggcccg tccaccgcgg cctccaggcg ctcgagccag
     2641 cgcggctcgc gcatcacggt gtcgttgtgc aggaagcaga gacggtcgcc cccggcgagc
     2701 ccggcaccct ggttgagcgc gcggatgagc cccacgttgt cggcgttgcg gtggtagcgc
     2761 agaggggtgc ccgccgccca ttcgcggaag aacgcctcgc taccgtcgga ggaggcgttg
     2821 tcgaccacgc acagctcgaa gggacgggtg gtcgaccgca ggctctcgag gcaggcgcgg
     2881 gtgaggccga gctggttgta gacgaccagg accacgctgg tggtccccgc gctcgtggcc
     2941 atcggctacc ggatggcccc cgccgcggcg atggccgcgg cccgccggct accgctcggc
     3001 gagcgggacg tacttgaggt cgatcgctcc cgtgtagtcg gagcggggcc ggatcagccg
     3061 gttgttgccg tgctgctcga gcacgtgcgc ggtccagccc gcgacccggc tcatcgcgaa
     3121 gatcggcgtg aactggtcgg tcgagatgcc catcgcggcg taggccgcgc ccgagtagag
     3181 atcgacgttg gggaagatgt gcttctcggc ccccatcacc cgcaccaccg tgtcgaggat
     3241 ctgcaccttg ctggtgtcac ccacctgctg gcccagctcg agcgccaggc gcttcaggtg
     3301 cttggcccgc gggtcctcca cccggtacac acggtggccg aaccccatga tgcgcgcctt
     3361 gtcggtgagc gccttgtgga tccacgcgtc ggccttggcc gggtccttga tcttctccac
     3421 catcagcatg acctgctcgt tggcgccacc gtggagcggg cccttgag
//
LOCUS       DATMCV010001065         5361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_463147, whole genome shotgun sequence.
ACCESSION   DATMCV010001065 DATMCV010000000
VERSION     DATMCV010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463147"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..984)
                     /locus_tag="VNU03_24430"
     CDS             complement(28..984)
                     /locus_tag="VNU03_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP77693.1"
                     /translation="MLVRFWGTRGSIPVAMTSAEVKRKLVTALVAAAGRGLDTPGKAR
                     AFVEEELAFSVSHTFGGNSSCVQIETGGPDYVLCDLGSGARVLGNNVLATGGPNGHRF
                     HVFMSHLHWDHIMGFPFFIPAYLPGNHVRIYGCHDALEEAFRRQNAAPSFPVDFSRMA
                     ARIEFIRLEPERDYDIAGLRVRAKRQHHGGDSYGYRIERAGKVVVYSTDSEHKQDDPK
                     EVEAFVEFFREADLVIFDAQYSLADAISVKEDWGHSSNVVGVEMCQLARAKRLCLYHH
                     EPIFDDERIAALLAETRRLEEITRGDHRVEIHAAYDGLEITL"
     gene            complement(994..1374)
                     /locus_tag="VNU03_24435"
     CDS             complement(994..1374)
                     /locus_tag="VNU03_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWP77694.1"
                     /translation="MDMELVSRRFADTVVLFPEGRIDHATAEQFKAALAPHLATCAEG
                     RDRVVIDMSGVEYISSVGLRVLMLASKQSKGQGCTLAVCGLQPVVREIFAISRFDLVL
                     QVFPTLHETLAALSAEALAAFGAA"
     gene            1445..2677
                     /locus_tag="VNU03_24440"
     CDS             1445..2677
                     /locus_tag="VNU03_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWP77695.1"
                     /translation="MEQSDLLKQLRIDRGPARVPRRRGRLLAILALLVLVAGVGLWLG
                     WARLRPPVVQTTAARAAAPGGAAGSVLDASGYVTARLQATVSAKITGKVTEVLIEEGM
                     RVREGAVLARLDDTEARAGLGLAQAQLVAARSQLAEVRAQLEQAERDYVRQQGLFDRQ
                     LVSPQSLDAALAQRDMLRARLANVQEQIKVAQESLAVAQVQLDNTVIRAPFSGVVIAK
                     SAQPGEMISPISAGGGFTRTGIGTIVDMDSLEIQVDVNESYINRVTPGQPVEATLNAY
                     PDWKIPGAVIAIIPTADRSKATVKVRIAIKTRDARIVPDMGVRVGFLESKPADAAPAP
                     TVAGVLVPAEAVRPDGNGSVVFVVGDDKKVQRRVVTLGPDVAGQRRVVSGLREGERVV
                     LAPPPGLADGQAVRLGDK"
     gene            2688..3377
                     /locus_tag="VNU03_24445"
     CDS             2688..3377
                     /locus_tag="VNU03_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77696.1"
                     /translation="MTVLVEIRGVTKVYQRGRERVEVLHGIDLDIPSGDFVALMGPSG
                     SGKTTLLNLIGGLDTPTGGSIVVQDRRIDQLSGGELAAWRAAHVGFVFQFYNLLPALS
                     AQRNVELPLLLTDLPAAERKRRARIALTLVGLEDRARHRPRELSGGQEQRVAIARAIV
                     SDPTLLVCDEPTGDLDRATADEVLRLLQMLNREHGKTVVMVTHDPKAAEYARRRVHLD
                     KGVMTGEPVAS"
     gene            3374..4537
                     /locus_tag="VNU03_24450"
     CDS             3374..4537
                     /locus_tag="VNU03_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77697.1"
                     /translation="MRYLPLLWAGLFRKKTRTILTLLSIVVAFALFGLLQAVEVAFES
                     GADAADAKRLLTTARYSIIEPLPMAYQRRIEQVPGVVGVAYADWFGAKYQNESNAFPV
                     FAVDPTRYLDMYPEFTIARAQRDAFAKTRTGAVVGKRLADRFGWHVGQKLPISSEIHP
                     KTDGSMNWEFDLVGIIDADDPAVRGNTDMVLINVAYFDEARQIGKGKTGWYIVRIADS
                     TQARGISSSIDTLFMNSPDETKTQPEKEFAIGFAKQIGDIGALVTRILIAVFFTILIL
                     TGNTMAQSIRERIPELAILKTLGFSDGKVTALVLAEAILLLILGGAIGMGAAVAAMPA
                     LNGSTGGRFPPLFVGPETWLLAAAIAGVVALIIGLPPALRANRLKIVDALSGH"
     gene            4544..>5361
                     /locus_tag="VNU03_24455"
     CDS             4544..>5361
                     /locus_tag="VNU03_24455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77698.1"
                     /translation="MRTLKQILAITLMNLRNLPQRVGTSLVVVVGIAGVVGVLVSVLA
                     MSEGFRHTLASTGRGDRVILLRAGSDAELSSGVDRDQATLLAALPGVARDAAGRPQAS
                     AELVVMVDLPRKGESNPNNVPFRGVQRAAFTIRDETRLLEGRRFEPGVREVMVGRKAA
                     GQFAGLAVGSRIAFRDSDWTVVGIFESGGDVHESEIWADAEVAMSAFRRSGYQSVTAR
                     LADGTDQGVATFRDTVARDPRFSISVLREPEDYAKQAGVLSSLINVLGYTVALF"
BASE COUNT          780 a         1920 c         1886 g          775 t
ORIGIN      
        1 cccggggtgc cggtcagctc ccgcccctca cagggtgatc tccagcccgt cgtaggcggc
       61 gtggatctcc acccggtggt cgccgcgggt gatctcctcc agccggcgcg tctcggccag
      121 gagagccgcg atgcgctcgt cgtcgaagat cggctcgtga tggtagaggc acaggcgctt
      181 cgcccgggcg agctggcaca tctccacgcc caccacgttg ctcgagtgcc cccagtcctc
      241 cttgaccgag atggcgtcgg cgagcgagta ctgcgcgtcg aagatcacca ggtccgcctc
      301 ccggaagaac tccacgaagg cctcgacctc cttcggatcg tcctgcttgt gctcggagtc
      361 ggtggagtac acgaccacct tgccggcccg ctcgatccgg tagccgtagg agtctccgcc
      421 gtggtgctgc cgcttggccc ggacgcgcag gcccgcgatg tcgtagtcgc gctcgggctc
      481 gagccggatg aactcgatcc gggcagccat ccgagagaaa tcgactggaa acgacggcgc
      541 cgcattctgc cgccggaagg cctcctcgag cgcgtcgtgg caaccgtaga tgcgcacgtg
      601 gttcccgggc aggtaggcgg gtatgaagaa cgggaacccc atgatgtggt cccagtgcag
      661 gtgcgacatg aagacgtgga accggtggcc gttcggtccg ccggtggcca gcacgttgtt
      721 gccgagcacc cgggcgccgc tgcccaggtc gcacagcacg tagtccgggc caccggtctc
      781 gatctgcaca caggaggagt tgcccccgaa ggtgtgcgag accgagaacg ccagctcctc
      841 ctcgacgaag gcgcgcgcct tgcccggggt gtcgagcccg cggcccgccg cggccaccag
      901 cgcggtcacc agcttgcgct tcacctccgc ggaggtcatg gccaccggga tcgagccgcg
      961 cgtgccccag aagcggacga gcatgccccg ggctcaggcg gccccgaagg cggcgagcgc
     1021 ctccgcggag agcgcggcga gcgtctcgtg cagcgtgggg aagacctgga ggaccaggtc
     1081 gaagcggctg atcgcgaaga tctcgcgcac caccggctgg agcccgcaca ccgcgagcgt
     1141 gcagccctgc cccttcgatt gcttggaggc gagcatgagc acgcgcaggc ccacgctgct
     1201 gatgtactcg accccgctca tgtcgatcac cacgcggtcc cggccctctg cgcacgtggc
     1261 caggtggggg gcgagcgcgg ccttgaactg ctccgcggtc gcatggtcga tgcgcccctc
     1321 cggaaagagg acgacggtgt cggcgaagcg cctcgagacc agctccatgt ccatgattcg
     1381 gggggcgatt ctatcaccat ggacacttgt ggcacactgg gacctcgtgc ctgaacccgc
     1441 aaccgtggaa cagtccgacc tcctgaagca gctccgcatc gaccgcggcc ccgctcgtgt
     1501 gccgcgccgg cgcggccgtc tgctggcgat cctggccctc ctcgtcctgg tggccggggt
     1561 cgggctctgg ctcggctggg cccgtctgcg tccgccggtg gtgcagacca cggcggcgcg
     1621 ggccgccgcg cccggggggg cggccggctc ggtgctggac gcgagcggtt acgtcaccgc
     1681 ccgcctccag gccaccgtct cggccaagat caccggcaag gtcaccgagg tgctgatcga
     1741 ggagggcatg cgggtccgcg agggcgcggt cctggcgcgt ctcgacgaca ccgaggcccg
     1801 cgccgggctc ggcctggccc aggcccagct cgtggccgcg cgctcgcagc tcgccgaggt
     1861 acgcgcccag ctcgagcagg ccgagcgcga ctacgtccgc cagcaggggc tcttcgaccg
     1921 gcagctggtg agtccgcaga gcctggacgc cgcgctcgcc cagcgggaca tgctgcgggc
     1981 ccggctcgcc aacgtccagg agcagatcaa ggtcgcgcag gagtcgctgg cggtcgcgca
     2041 ggtgcagctc gacaacaccg tcatccgcgc tccgttcagc ggggtggtga tcgccaagtc
     2101 cgcgcagccg ggcgagatga tctcgccgat ctcggcgggc gggggcttca cccgcaccgg
     2161 catcgggacc atcgtggaca tggactccct cgagatccag gtggacgtca acgagtccta
     2221 catcaaccgc gtgactcccg ggcagccggt ggaggccacg ctgaacgcgt atcccgactg
     2281 gaagatcccc ggcgcggtga tcgcgatcat cccgaccgcg gaccggagca aggccaccgt
     2341 caaggtgcgg atcgcgatca agacgcgcga cgcgcggatc gtgcccgaca tgggcgtgcg
     2401 ggttggcttc ctcgagtcca agcccgcgga cgcggcgccg gcccccaccg tcgcgggggt
     2461 gctggtgccg gcggaggcgg tgcgcccgga cggcaacggc tccgtcgtgt tcgtggtggg
     2521 cgacgacaag aaggtgcagc gccgggtggt gaccctgggc cccgacgtgg ccgggcagcg
     2581 ccgcgtggtg agcggcctgc gcgagggcga gcgcgtcgtg ctcgcgccgc cgcccgggct
     2641 cgcggacggc caggcggtgc ggctcgggga caagtgagca cgcgcgcgtg acggtcctgg
     2701 tcgagatccg cggggtgacc aaggtctacc agcgcgggcg cgagcgggtc gaggtcctgc
     2761 acggcatcga cctcgacatc ccgagcggcg acttcgtggc cctgatgggc ccgtccgggt
     2821 ctggcaagac cacgctgctc aacctgatcg gcggactcga cacgcccacc ggcgggagca
     2881 tcgtggtgca ggaccggcgc atcgaccagc tctcgggcgg ggagctggcg gcctggcgcg
     2941 cggcccacgt gggcttcgtc ttccagttct acaacctgct gcccgcgctc tccgcgcaac
     3001 gcaacgtgga gctgcctctg ctgctgaccg acctgcccgc ggcggagcgg aagcggcgcg
     3061 cgcggatcgc gctcaccctg gtcggcctcg aggaccgcgc gcgccaccgg ccccgcgagc
     3121 tctcgggcgg gcaggagcag cgcgtggcca tcgcgcgggc catcgtctcc gaccccaccc
     3181 tgctcgtctg cgacgagccc accggcgatc tcgatcgggc caccgcggac gaggtgctgc
     3241 ggctgctcca gatgctcaac cgcgagcacg gcaagacggt ggtgatggtc acccacgatc
     3301 cgaaggcggc cgagtacgcg cgccggcgcg tgcacctcga caagggggtc atgaccgggg
     3361 agccggtggc gtcgtgagat acctgcccct gctctgggcc ggcctcttcc gcaagaagac
     3421 gcgcaccatc ctgaccctgc tctcgatcgt ggtggccttc gcgctcttcg gcctgctcca
     3481 ggcggtcgag gttgccttcg agtcgggcgc ggacgcggcc gacgccaagc gcctgctcac
     3541 caccgcgcgc tactcgatca tcgagccgct gccgatggcc taccagcgcc gcatcgagca
     3601 ggtaccggga gtggtgggcg tcgcctatgc ggactggttc ggggccaagt accagaacga
     3661 gtcgaacgcc ttcccggtct tcgcggtgga cccgacgcgc tacctcgaca tgtaccccga
     3721 gttcaccatc gcgcgcgcgc agcgggacgc cttcgccaag acgcgcaccg gggcggtggt
     3781 gggcaagcgc ctggccgatc gcttcgggtg gcacgtgggc cagaagctcc cgatcagctc
     3841 cgagatccac ccgaagacgg acggcagcat gaactgggag ttcgacctgg tgggcatcat
     3901 cgacgccgac gaccccgcgg tgcgcggcaa caccgacatg gtgctgatca acgtggccta
     3961 cttcgacgag gcgcgccaga tcgggaaggg caagaccggc tggtacatcg tgcgcatcgc
     4021 ggactcgacc caggcccggg gcatctcgag ctcgatcgac accctcttca tgaactcgcc
     4081 cgacgagacc aagacccagc ccgagaagga gttcgcgatc ggcttcgcca agcagatcgg
     4141 cgacatcggc gcgctcgtga cccgcatcct catcgcggtg ttcttcacca tcctgatcct
     4201 gaccggcaac accatggccc agtccattcg cgagcgcatc ccggagctgg ccatcttgaa
     4261 gacgctcggc ttctcggacg gcaaggtcac cgcgctggtg ctggcggagg cgatcctgct
     4321 cctgatcctg ggcggcgcga tcggcatggg cgcggcggtc gcggccatgc ccgcgctcaa
     4381 cggctccacc ggaggccgct tccccccgct cttcgtcggc cccgagacct ggctcctcgc
     4441 cgccgccatc gccggcgtcg tcgccctcat catcggcctt ccccccgccc tgcgcgcgaa
     4501 ccggctcaag atagtcgacg ccttgtcagg gcactagact cacatgcgca ccctgaagca
     4561 gattctcgcc atcaccctca tgaacctgcg caacctcccg cagcgcgtgg gcacgtccct
     4621 ggtggtcgtg gtcggcatcg cgggcgtggt gggcgtgctg gtgtcggtgc tggccatgtc
     4681 ggagggcttc cgccacaccc tggccagcac cgggcggggc gaccgcgtca tcctcctgcg
     4741 cgccggctcg gacgcggagc tgtcgagcgg ggtcgaccgc gaccaggcca cgctcctggc
     4801 cgccctgccc ggcgtcgccc gcgacgcggc cgggcggcca caggcctcgg ccgagctggt
     4861 ggtcatggtg gacctgcccc gcaagggcga gagcaacccc aacaacgtgc cgttccgcgg
     4921 ggtgcagcgc gccgcgttca cgatccgcga cgagacgcgc ctgctcgagg gccggcgctt
     4981 cgagcccggc gtccgcgagg tgatggtcgg gcggaaggcg gccggccagt tcgccggcct
     5041 cgcggtcggc tcgcgcatcg ccttccgcga cagcgactgg acggtggtgg ggatcttcga
     5101 gagcggcggc gacgtgcacg agtccgagat ctgggccgac gcggaggtgg cgatgtcggc
     5161 cttccggcgc tccggctacc agtcggtgac cgcgcgcctc gccgacggca ccgaccaggg
     5221 cgtggccacg ttccgggaca cggtcgcccg cgacccgcgc ttctcgatca gcgtgctccg
     5281 cgagccggag gactacgcca agcaggcggg cgtgctttcc agcctcatca acgtgctcgg
     5341 ctacacggtg gccttgttca t
//
LOCUS       DATMCV010001066         6028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_524209, whole genome shotgun sequence.
ACCESSION   DATMCV010001066 DATMCV010000000
VERSION     DATMCV010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524209"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..1469
                     /gene="dctP"
                     /locus_tag="VNU03_24460"
     CDS             333..1469
                     /gene="dctP"
                     /locus_tag="VNU03_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HWP77699.1"
                     /translation="MTGDADPGTQVTTDRRALLKATAAAAGAAALAFPAVLRGQTPVK
                     WRIQTAWDAGTAGYTAFQKFCASVKEISEGKLLFQPFPAGSIVGTFEMFDAVKSGVFD
                     AMHVFTTYWAGKMPVAAFLSSYPLGLDRPDQWETWFYELGGLQIARKAYRANNMFYVG
                     PIQHDLNLIHSRVPIRSFEEFKGKKIRYPGGMIAEVFSYAGVSTSILPGAEVYQALEK
                     GVIDAADFVGPAVNYNLGFAEVAKYIIMGPPSTPCLHQPVDLMDLTVNMAKWTALPKH
                     LQELVIAVTRQHSWDQYAYIQKENIAAWDKYKAKGVQIIRLSEDDVQKFRRFAIPMWF
                     KWAKKDALAKDAFASQLAFMKTISVGYIPDSMLVDIDGKTKLTL"
     gene            1483..3132
                     /locus_tag="VNU03_24465"
     CDS             1483..3132
                     /locus_tag="VNU03_24465"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP77700.1"
                     /translation="MTADLHDLSIAALSKLIAERRLSPVELVDALIQRVEQHDAQTRA
                     FITRTFDLAQRQARQAEAEISAGRSRGPLHGVPFGLKDIYDTADILTSAHSRIFIDRV
                     PAEDATTTAKLYEAGAVLLGKLATHEMAHAGPSFDLPWPPARNPWNLAHFTGGSSTGS
                     GAAVAAGMVPVALGSDTGGSIRGPASHCGVVGLMPTFGLVSRAGVITNSYTFDHCGPL
                     TRTVEDAALVLQALAGHDPKDAGSLSRPIPRYVDALGPDLRGLRIGVLRHHWEDDIPA
                     SEDVRKAMDASLDVLRRLGAELEECRVRPLGAYYDVKIIIAETEIFSVHQRDLIARPD
                     EFGADFRSRALPAVLFTANDYVQATREHRRMMVEMEPLYGRYDAFVTCGLGEAPRLTD
                     YRSVSFWQKASLLTAWNVTGQPVLSLPNGFGRNGLPLGMQILGRPFDEPTILRVGHAY
                     ERATEWHRRRPALVPGAEAPAVTPPPVLSVPADQADARTRDLCAQAARRAGLKLDDLM
                     LAQLLEGAPYALAMAERLRRDHGLGHEPANVFTFSPTRLSD"
     gene            3141..3965
                     /locus_tag="VNU03_24470"
     CDS             3141..3965
                     /locus_tag="VNU03_24470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77701.1"
                     /translation="MLPELQERDATGDVAVIYAEIRRLWAVPYVSSLQRHLATRPGWL
                     EWTWASLAPAFTSGQAQTAARHAVEGLEVPRLAPVSRDALRVWGVDADGEAAIRATCA
                     SFVRVSPINLMLSGLLRGLLRGERPSGAAGDETALATPAPLGPLPPLVDPAALPAPQQ
                     AVLASLSTGVSGEPFVPGLYRMLGAWPGYLAHVATVIAPLRDDAATRSACQQLLAAVD
                     AAIPAVFAGLPPLPAAPPRPPADDVPAVLAALGTYRKTSPEMVVFGRMLGDALPPP"
     gene            3985..4743
                     /locus_tag="VNU03_24475"
     CDS             3985..4743
                     /locus_tag="VNU03_24475"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWP77702.1"
                     /translation="MNIRPNRVKQRLASGQVATILAGTNDPDLIDQLGPLGADGIWLE
                     GEHGGVDYADLGNLTRACDLWSVTSVVRVMDNDYATIYRTLDRGAQGIVVPHVNTRAD
                     AQAAVEAAKFAPLGKRGMFTSRQGFAVTDYLKTANDQSLLIVLIEDIVAVNNLDDILK
                     TDHIDVFFVAPNDLATSMGHIGKLDLPEVQKTVDGAITKIVQSGRKAGTLVSSGNVER
                     YTRLGVRCIMSSFFPWIQAGAKDLMDRAAAGAKG"
     gene            complement(4740..>6028)
                     /locus_tag="VNU03_24480"
     CDS             complement(4740..>6028)
                     /locus_tag="VNU03_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HWP77703.1"
                     /translation="SQAVARFGRLDLETVMQPAIRHAERGFRATPYLVAAVTETAPDL
                     ARFPETARTFLPGGAQIKVGDLITMPEYAATLRLIAAKGPAALYGGDLGAAVADYMRR
                     DGGIITLDDLARYETIERQPVRGRYRGFEVTGAPPPTAGGLHLIEMLNILEGFDPRAL
                     GFGTAEYFHLIAEVLKIGFADRNACTGDPAFVDIPVERLISKEHAAARRAQIRSDRAG
                     EYGAAAGVAAGSAHTTHVTTADADGNVVAMTQTINSLFGSKATVPGTGILLNNTMALF
                     DPHPGTPASIAPGKRVTSSMAPTIVLRDGKPAWALGLPGGVRIFTSVLQAVVNLIDHR
                     MTLQEAVEAPRIWSQGQELEMEQDIPAAVRDAVAARGHRVVPVGGVAGGMNAVAFAAD
                     GTLEGAACWRADGTPIGVSGGLARPGSRFRPEVSRR"
BASE COUNT          872 a         2262 c         2050 g          844 t
ORIGIN      
        1 ccgtgcaccg aggcccggat gcgcgcgacc cggcgcacca gcgcccccag gggcgagtag
       61 gcgaggagga ggcgcgcgat cctctccccc tgccccggcg tcggcgccgg ggccggatcg
      121 ctggacacgg cacgactctc gatctggtat gcggacatgc ggcgggctcc tgcgacgcgg
      181 cggatgctat cagggtggca tcggtgggtc aatgcgaggc cgtccacccg cttccttgac
      241 aggccaggcg tccccgctcg ataatccccg cgcactcact cacgtcgatt cgggcgcttg
      301 ccgcccacgc tcccaacgaa gggaacacac gcatgacagg cgacgccgac ccggggacgc
      361 aggtgaccac cgatcggcgc gcgctcctga aggccaccgc ggccgcggcc ggagccgccg
      421 ctctcgcgtt ccccgcggtc ctccgggggc agaccccggt caagtggcgc atccagaccg
      481 cgtgggacgc gggcaccgcc ggctacaccg cgttccagaa gttctgcgcg agcgtgaagg
      541 agatctcgga gggcaagctc ctgttccagc ccttcccggc cggctccatc gtcggcacct
      601 tcgagatgtt cgacgcggtg aagagcggcg tgttcgacgc catgcacgtc ttcaccacct
      661 actgggccgg gaagatgccg gtcgccgcgt tcctctcctc gtacccgctg ggcctcgacc
      721 ggccggacca gtgggagacg tggttctacg agctgggtgg gctccagatc gcccgcaagg
      781 cctaccgggc caacaacatg ttctacgtgg gcccgatcca gcacgacctg aacctgatcc
      841 attccagggt cccgatccga tccttcgagg agttcaaggg caagaagatc cggtacccgg
      901 gcggcatgat cgcggaggtc ttctcgtacg ccggcgtctc gacgtcgatc ctgcccggcg
      961 ccgaggtcta ccaggccctg gagaaggggg tcatcgacgc ggcggatttc gtcgggccgg
     1021 cggtgaacta caacctgggc ttcgccgagg tcgccaagta catcatcatg gggccgccct
     1081 cgaccccgtg cctccaccag ccggtggacc tcatggacct caccgtcaac atggcgaagt
     1141 ggaccgccct gcccaagcac ctccaggagc tggtgatcgc ggtgacccgg cagcactcct
     1201 gggatcagta cgcctacatc cagaaggaga acatcgcggc ctgggacaag tacaaggcca
     1261 agggcgtcca gatcatccgg ctctccgagg acgacgtgca gaagttccgg cgcttcgcga
     1321 tcccgatgtg gttcaagtgg gccaagaagg atgcgctcgc caaggacgcc ttcgcgtccc
     1381 agctcgcgtt catgaagacg atcagcgtcg gctacatccc ggactccatg ctggtcgaca
     1441 tcgacggaaa gaccaagctc accctctgag cgcgcccgac ccatgaccgc cgatctccac
     1501 gatctgtcga tcgccgcgct gtcgaagctg atcgcggagc gccgtctctc cccggtcgag
     1561 ttggtggacg cgctgatcca gcgcgtcgag caacacgacg cacagacccg cgccttcatc
     1621 acccgaacgt tcgacctggc ccagcggcag gcccggcagg cggaggccga gatctcggcg
     1681 ggccggtcgc gcggcccgct ccacggcgtt ccgttcgggc tcaaggacat ctacgacacc
     1741 gcggacattc tcacctcggc ccattcgcgg atcttcatcg accgcgtccc ggccgaggac
     1801 gcgaccacga ccgccaagct ctacgaggcc ggcgcggtcc tgctcggcaa gctcgccacc
     1861 cacgagatgg cccacgccgg gccgtcgttc gacctgccgt ggccccccgc gcgcaacccg
     1921 tggaacctgg cgcacttcac cggcggctcc tccaccggct cgggcgccgc ggtggccgcg
     1981 ggaatggtcc cggtggcgct cggctcggac accggcggct cgatccgcgg cccggcctcc
     2041 cactgcggcg tggtcgggct catgcccacg ttcgggctgg tgagccgcgc cggcgtcatc
     2101 accaactcct acacgttcga ccactgcggc cccctgaccc gcaccgtgga ggacgccgcg
     2161 ctcgtgctcc aggcgctggc cggccacgac ccgaaggacg cgggcagcct gagtcgcccg
     2221 atcccccgct acgtcgacgc gctcggcccg gacctgcgcg ggctccggat cggggtgctg
     2281 cgccaccact gggaggacga catcccggcg tccgaggatg tgcggaaggc catggacgcc
     2341 tcgctcgacg tgctccgccg gctgggggcg gagctcgagg agtgccgcgt gcggccgctc
     2401 ggcgcctact acgacgtcaa gatcatcatc gcggagaccg agatcttcag cgtccaccag
     2461 cgggacctga tcgcgcggcc ggacgagttc ggcgccgact tccgctcgcg cgcgctgccc
     2521 gcggtgctct tcaccgccaa cgactacgtc caggccaccc gcgagcaccg gcggatgatg
     2581 gtcgagatgg agccgctcta cgggcgctac gacgccttcg tcacctgcgg cctcggcgag
     2641 gcgccccggc tgaccgacta tcgcagcgtg tcgttctggc agaaggcgag cctgctcacc
     2701 gcctggaacg tcaccggcca gccggtcctc tcgctgccga acggcttcgg ccggaacggg
     2761 ctgccgctcg gcatgcagat cctcgggcgg ccgttcgacg agccgaccat tctccgcgtc
     2821 gggcacgcct acgagcgggc caccgagtgg caccggcgcc ggcccgcgct cgtgccggga
     2881 gccgaggcgc ccgcggtcac gcccccgccc gtcctctcgg tcccggcgga tcaggccgac
     2941 gcccgcacgc gcgatctctg cgcccaggcc gcacgccgcg ccggcctcaa gctcgacgac
     3001 ctgatgctcg cccagctcct ggaaggcgcc ccgtacgcgc tcgcgatggc cgagcgccta
     3061 cgccgcgacc acgggctcgg gcacgagccc gcgaacgtct tcaccttctc gcccacccgc
     3121 ctctccgact agagcgccgc gtgctgccgg agctacagga gcgtgacgcc accggtgacg
     3181 tcgcggtgat ctacgccgag atccgacggc tctgggccgt cccctacgtc tcctcgctgc
     3241 agcgccacct cgccacgcgt ccgggctggc tcgagtggac ctgggcttcc ctggccccgg
     3301 ccttcacgag cgggcaggcc cagaccgcgg cccggcacgc ggtggagggc ctcgaggtgc
     3361 cacggctcgc gccggtgtcg cgggacgccc tgcgcgtctg gggcgtcgac gccgacgggg
     3421 aggcggcgat ccgggcgacc tgcgcgagct tcgtccgcgt gagcccgatc aacctgatgc
     3481 tctccgggct cctgcgcggc cttctgcgtg gggagcgccc gtcgggcgca gccggcgacg
     3541 agaccgccct cgccacaccc gcgccgctcg gaccgctgcc ccccctcgtc gaccccgccg
     3601 ccctgcccgc gccgcagcag gcggtgctcg cgtcgctcag caccggcgtg agcggcgagc
     3661 ccttcgtgcc aggcctctac cgcatgctcg gggcctggcc gggttacctc gcccacgtgg
     3721 cgaccgtgat cgctccattg cgggacgacg ccgcgacccg gtcggcctgc cagcaactcc
     3781 tggccgcggt ggacgcggcg atcccggcgg tcttcgcggg gctgccgccg ttgcccgccg
     3841 cgccgccgcg gccgccggcc gacgacgtcc ccgcggtgct ggccgcgctc ggcacctatc
     3901 ggaagacgag cccggagatg gtagtcttcg gccggatgct cggcgacgcg ctgcccccgc
     3961 cctgaccgga gagaggaccc ctcgatgaac attcgaccca accgcgtgaa gcagcgcctc
     4021 gcgtcaggcc aggtggccac cattctggcc ggcaccaacg acccggacct gatcgaccag
     4081 ctcgggccgc tgggggccga cggcatctgg ctcgagggcg agcacggcgg cgtcgactac
     4141 gccgatctgg ggaacctgac ccgggcctgc gacctctgga gcgtgaccag cgtggtgcgg
     4201 gtcatggaca acgactacgc gacgatctac cgcacgctcg accgcggggc ccagggcatc
     4261 gtggtgccgc acgtcaatac ccgcgcggac gcccaggccg cggtggaggc ggccaagttc
     4321 gcaccgctcg gcaagcgcgg catgttcacg agccgccagg gcttcgcggt gaccgactac
     4381 ctcaagaccg ccaacgacca gagcctgctg atcgtgctga tcgaggacat cgtggcggtg
     4441 aacaacctcg acgatattct caagacggat cacatcgacg tcttcttcgt ggcccccaac
     4501 gacctcgcga cctccatggg ccacatcggc aagctggacc tgccggaggt gcagaagacg
     4561 gtggacgggg cgatcaccaa gatcgtccag tccggccgca aggcgggcac cctggtgagc
     4621 tccggcaacg tcgagcgcta cacccgtctc ggcgtgcggt gcatcatgag cagcttcttc
     4681 ccgtggatcc aggccggcgc gaaggacctg atggaccgcg ccgcggcggg ggcgaagggc
     4741 tagcgccggc tcacctcggg ccggaagcgg ctccccggcc gcgcgagccc gccgctcacc
     4801 ccgatggggg tgccgtcggc gcgccagcac gccgcgccct ccagcgtgcc gtccgccgcg
     4861 aaggccaccg cgttcatccc acccgcgacg ccgccgaccg gcaccacgcg atggccgcgc
     4921 gcggcgaccg cgtcccggac cgccgcggga atgtcctgct ccatctccag ctcctgcccc
     4981 tggctccaga tccgcggcgc ctccaccgcc tcctgcagcg tcatgcggtg gtcgatcagg
     5041 ttgaccaccg cctgcagcac ggaggtgaag atgcgcacgc cgccgggcag gccgagcgcc
     5101 cacgccggct tgccgtcgcg cagcacgatc gtgggcgcca tgctgctggt cacccgcttg
     5161 cctggagcga tggaggcagg tgtccccggg tgtgggtcga agagcgccat ggtgttgttc
     5221 agcaggatgc cggtgccggg gacggtggcc ttcgacccga acaggctgtt gatggtctgg
     5281 gtcatcgcga ccacgttgcc gtccgcgtcg gcggtcgtga cgtgggtggt gtgagcggag
     5341 ccggcggcca ccccggccgc cgcgccgtac tcccccgccc gatcggagcg gatctgcgcg
     5401 cgccgcgcgg cagcgtgctc cttcgagatg agccgctcga ccgggatgtc cacgaacgcg
     5461 ggatcgccgg tgcacgcgtt gcggtccgcg aagccgatct tgagcacctc ggcgatcagg
     5521 tggaagtact cggcggtgcc gaagccgagc gcgcgcggat cgaagccctc gaggatgttc
     5581 agcatctcga tcaggtggag cccgcccgcg gtgggcggcg gggcgcccgt gacctcgaag
     5641 ccgcgatagc gcccgcgcac cggctgccgc tcgatcgtct cgtagcgcgc cagatcgtcc
     5701 agggtgatga tcccaccgtc ccggcgcatg tagtcggcca ccgccgctcc caggtcgccg
     5761 ccgtagagcg ccgcggggcc cttcgccgcg attaggcgca gggtggccgc gtactccggc
     5821 atcgtgatca ggtcgcccac cttgatctgc gcgccacccg gcaggaacgt ccgcgcggtc
     5881 tcggggaagc gcgccaggtc gggcgcggtc tcggtgaccg ccgccaccag gtagggcgtg
     5941 gcgcggaagc cgcgctccgc gtggcggatg gcgggctgca tcacggtctc gaggtcgagg
     6001 cgcccgaagc gggccaccgc ctgcgacc
//
LOCUS       DATMCV010001067         3372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_857744, whole genome shotgun sequence.
ACCESSION   DATMCV010001067 DATMCV010000000
VERSION     DATMCV010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857744"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VNU03_24485"
     CDS             complement(<1..697)
                     /locus_tag="VNU03_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP77704.1"
                     /translation="MESSAVELRDVGKRFGSVVALDRVDLEIRKGEFFSLLGPSGCGK
                     TTTLNLIGGFETASRGTLLIDGQPMGDTPSHARPVNTVFQSYALFPHMSVADNVGFGL
                     RMKKVPRPERRRAVEEMLRLVSLEGFEARRPGQLSGGQRQRVALARALINHPSVLLLD
                     EPLGALDLKLRKQMQAELTRLQRRVGITFVYVTHDQDEAMSMSDRIAVMDRGRILQIG
                     TPAEIYRDPADRFV"
     gene            complement(758..1993)
                     /locus_tag="VNU03_24490"
     CDS             complement(758..1993)
                     /locus_tag="VNU03_24490"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HWP77705.1"
                     /translation="MKITLRLVNAAIGLLAVLILIGAPGAHADVVTDANTRAADVVSR
                     VPSTPIAVRMMAIVQVSVFEAVNAVTGRYPPQRAKLTPAPGASVEAAVAAATRTALSK
                     LVPAQQAAVDADYQALLASVSDGPAKTAGIALGEQAATAIVALCADDGAGAPDVYRPH
                     AGAGVYVPTVGPAVPHWGGRRPWVMPRGDHFRPGPPPALTSDTWARDYNEIKAVGSRN
                     STQRTPEQTAMAKFWEATAPAVYWPVARSVAGMPGRDVTDNARLFALAAMAMDDGLIA
                     VFDAKYTYNLWRPVTAIRNGDLDGNNATDRDPGWTPFIDTPLHPEYPCAHCIVSASLG
                     AVLEAELGSGPTPTLSSASSTAGGAVRTWASVGNFTKEVAEARIYDGVHYRTSTDVGS
                     AMGKKIGELAAKSVPRATR"
     gene            2121..2822
                     /locus_tag="VNU03_24495"
     CDS             2121..2822
                     /locus_tag="VNU03_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP77706.1"
                     /translation="MKGYGQFCPIAMACETFAERWTPLILRELLAGSRRFNEIRQGLP
                     LISRTLLGQRLRELEAAGVVQSQPLARGRGREYRPTPAAEEFRGVLERLGEWGQRWAT
                     AQFDPDNLDLMLLMWNVRRRIAFDRLPDRRIVVRFDFRAFPSRCRSLRTSWLILERSG
                     SDVCLKDPGFDVDLVVAADAGAMARVWTGALTFAQAVRDGGLRVEGPRDLVRAFPTWL
                     LLSHFAHVERPARAV"
     gene            2827..>3372
                     /locus_tag="VNU03_24500"
     CDS             2827..>3372
                     /locus_tag="VNU03_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWP77707.1"
                     /translation="MARLRPWALVLLACVVACATPAPPAPDTPAKAEAPGPRFAAGGP
                     AADELGAAEGYPKGSRATFYSVGSAVGSHSHMDEIFPSRLVHKAAEPSRLGRVAEPRI
                     PALDDYLARSLTTGLLIARGDTILVERYQYDRTDRQRFTSWSMAKTVTAMLVGIAIAE
                     GHIRSVDDQAAAYVPELAGTEY"
BASE COUNT          445 a         1209 c         1228 g          490 t
ORIGIN      
        1 tcacgaagcg gtcggcgggg tcgcggtaga tctccgccgg cgtgccgatt tgcaagatcc
       61 ggccccgatc catcaccgcg atgcggtcgg acatggacat cgcctcgtct tggtcgtggg
      121 tcacgtacac gaaggtgatc ccgacccggc gctgcagccg cgtgagctcg gcctgcatct
      181 gcttgcggag cttgagatcg agggcgccga gcggctcgtc gagcaggagc accgacgggt
      241 ggttgatcag ggcgcgcgcc agcgccaccc gctggcgctg cccgccgctc agctggcccg
      301 gccgccgcgc ctcgaacccc tcgagcgaga ccaggcgcag catctcctcc accgcgcgcc
      361 gccgctcggg ccgcggcacc ttcttcatgc gcaagccgaa ccccacgttg tcggccacgc
      421 tcatgtgcgg gaagagcgcg tagctctgga acacggtgtt cacgggccgc gcgtgcgacg
      481 gggtgtcccc catcggctgc ccgtcgatca ggagcgtgcc gcggctggcg gtctcaaagc
      541 cgccgatcag gttgagggtg gtggtcttgc cgcagccgct cggccccagc agcgagaaga
      601 actcgccctt gcggatctcg aggtcgacgc ggtccagcgc caccacggag ccgaagcgct
      661 tgcccacgtc gcgcagctcg accgcagagc tctccatggg gatccgcggc gagcgtactc
      721 gggccgcgtc acccctgtca acaggcgcgg gacggcgtca gcgagtcgct ctcggcacgc
      781 tcttcgcggc cagctcgccg atcttcttgc ccatcgcgct gcccacgtcg gtcgaggtgc
      841 ggtagtgcac gccgtcgtag atgcgcgcct ccgcgacctc cttcgtgaag ttgccgacgc
      901 tcgcccacgt gcggaccgcg ccccccgcgg tcgagctggc ggagctgagc gtcggcgtgg
      961 ggccggaccc gagctcggcc tcgagcaccg cgcccaggga cgccgacacg atgcagtggg
     1021 cgcacggata ctcggggtgc agtggcgtat cgatgaacgg ggtccagccc ggatcccggt
     1081 cggttgcgtt gttgccgtcg agatcgccgt tgcggatggc ggtgaccggt cgccagagat
     1141 tgtaggtgta cttggcgtcg aagaccgcga tcagcccgtc gtccatggcc atcgcggcca
     1201 gcgcgaacag gcgcgcgttg tcggtcacgt cgcggccggg catgccggcg accgagcgcg
     1261 cgaccggcca gtagaccgcg ggagccgtcg cttcccagaa cttcgccatc gcggtctgct
     1321 ccggcgtgcg ctgggtgctg ttccgcgacc cgaccgcctt gatctcgttg tagtcgcgcg
     1381 cccaggtgtc gctcgtgagc gcgggcgggg ggcccgggcg gaagtgatcg ccacggggca
     1441 tcacccacgg acggcgcccg ccccagtggg gcaccgccgg ccccaccgtg ggcacgtaga
     1501 cgcccgcgcc cgcgtgcggg cgatagacgt cgggggcgcc cgcgccgtcg tcggcgcaga
     1561 gcgccacgat cgcggtcgcg gcctgctcgc cgagcgcgat acccgcggtc ttcgcggggc
     1621 cgtcggagac ggatgcgagc agggcctggt agtcggcgtc gacggccgcc tgctgggccg
     1681 gcaccagctt cgagagcgcg gtgcgcgtcg ccgccgcgac cgcggcctcc accgacgcgc
     1741 cgggcgcggg ggtgagcttg gcgcgctgcg gaggatagcg accggtcacc gcgttgaccg
     1801 cttcgaacac cgagacctgg acgatggcca tcatccgcac cgcgatgggc gtcgacggga
     1861 cgcgcgacac gacgtcggcg gcccgggtgt tggcatcggt gacgacgtcc gcgtgggcgc
     1921 ccggggcacc gatgaggatc aagacggcca ggagtccgat cgctgcgttg acgagccgga
     1981 gggtgatctt catgtttgtt gctcctttcg cttccggacc cggtctctga gtgcccacac
     2041 aatggcaggc cgccccggtg gtgccaagac cataaaatgg acttgcccgg tcggcgcggg
     2101 gcgcctagag tcgggccacc atgaagggct acggccagtt ctgcccgatc gcgatggcgt
     2161 gcgagacctt cgccgagcgc tggacgccgc tgatcctgag agagctgctg gcgggatcgc
     2221 ggaggttcaa cgagatccgg cagggcctgc ccctgatctc gcggaccctg ctcgggcaac
     2281 ggctgcgcga gctggaggcg gcgggcgtcg tccagagcca gccgctcgcc cgcggccggg
     2341 gacgggagta ccggccgacg cccgcggccg aggaattccg cggagtcctg gagcgcctcg
     2401 gggagtgggg gcagcggtgg gccaccgccc agttcgaccc cgacaatctc gacctgatgc
     2461 tgctcatgtg gaacgtgcgc cggcgcatcg ccttcgaccg gcttcccgac cgacgcatcg
     2521 tcgtgcgctt cgactttcgc gcgttcccgt cgcgctgccg gtccttgcgg acgtcctggc
     2581 tgatcctcga gcgcagcgga tctgacgtgt gcctgaagga tcccgggttc gatgtcgacc
     2641 tggtggtcgc ggccgacgcg ggcgccatgg cccgcgtgtg gaccggggcc ctcacgttcg
     2701 cgcaggcggt ccgggacggc ggcctgcggg tggagggccc ccgcgacctc gtccgcgcgt
     2761 ttccgacctg gctcctcctg agccacttcg cccacgtcga gcggcccgcc cgcgccgtct
     2821 agagccatgg cgcggctccg gccgtgggcg ctcgtcctcc tcgcgtgcgt ggtcgcctgc
     2881 gcgaccccgg cgccgccggc gccggacacg ccggccaagg ccgaagcgcc cgggccgcgc
     2941 ttcgcggccg gcggacccgc cgcggacgag ctgggcgcgg cggagggcta cccgaaaggc
     3001 agccgcgcga cgttctacag cgtcgggtcc gcggtcggct cgcacagcca catggacgag
     3061 atcttcccga gccgcctcgt ccacaaggcg gcggagccct cgcgcctcgg gcgggtcgcc
     3121 gagccgcgca tcccggcgct ggacgactac ctggcgcgga gcctgacgac cgggctgctg
     3181 atcgcgcggg gcgacaccat cctcgtcgag cgctaccagt acgaccgcac cgaccggcag
     3241 cgcttcacgt cgtggtcgat ggccaagacc gtgaccgcca tgctcgtggg catcgcgatc
     3301 gccgagggcc acatccgctc ggtcgacgat caggcggcgg cctacgtgcc cgagctggcc
     3361 ggcaccgagt ac
//
LOCUS       DATMCV010001068         5108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_126106, whole genome shotgun sequence.
ACCESSION   DATMCV010001068 DATMCV010000000
VERSION     DATMCV010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126106"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VNU03_24505"
     CDS             <1..617
                     /locus_tag="VNU03_24505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77708.1"
                     /translation="LHGDMATVDAPDRVVGEAVARFGGLDGLVSNAGVNRPGPLVSYA
                     VEDWDRMFAVNTRATWLLAKAAHDALAAAKGAIVAVGSMSGSNAHANLGPYGPSKAAV
                     IMLVSVLAQEFGRDGIRVNSLSPGMVRTGMTEKVYADQTVASARDALVPIGRVATPED
                     MADVIAFLLGPDARYVNGHDLVVDGGVTGNFLGRLPGIGQITRS"
     gene            887..1672
                     /locus_tag="VNU03_24510"
     CDS             887..1672
                     /locus_tag="VNU03_24510"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWP77709.1"
                     /translation="MGAALGLIVLFAVGATWTAEPERIIARAEPVEEVEEVETPEYDL
                     AAIPDSIKAAAERHRLPESLISAVIFVESGFDHAAVSSKGARGLMQLMPATSAMIGVS
                     DPHNPEQNIDAGASHLRAMLDTFKGDLPLALAAYNAGEGNVVRYHGIPPYPETRRFVA
                     RVLRRMGDKPGAERVMAKPVPTPVWTSRTSRPAPAVRMAVSPPSSSRPTMVPFPAARA
                     ERASHVVYQDDDARPQIRSWTSSPPGPAASLVPEPTQPFAESP"
     gene            1757..3049
                     /locus_tag="VNU03_24515"
     CDS             1757..3049
                     /locus_tag="VNU03_24515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77710.1"
                     /translation="MRFGVFYEHQLPRPWAAGSEQQLFQDALAQVELADRLGIDYAWE
                     VEHHFLEEYSHSSAPEVFLAAASQRTRRIRLGHGIVLMPPGYNHPARVAERLATLDLV
                     SNGRVDWGTGESASRAELEGFGVESAERRAMWRETTEQVANMLALDPYPGFQGKYFSM
                     PTRNVVPKPVQKPHPPLWVACSNRETIHLAAQLGMGALTFAFIDPAEARHWVDDYYET
                     FKRECVPIGHAVNPNVAMVTGFSCHPDAQEARRRGIDGFRFFQFALGHHYVFGKHQPG
                     RTDIWDKFERVRAELDLEVLGGGTGCIGTPDELRASLRAFEEYGVDQTIFIQQGGKNR
                     HEHICESLELFAGAVMPEFKAREAERAARKAEALAPYVAAAFARKQAMAPLADADIPA
                     YEAYGLTVTQPVDLDKMPEANRRRVLTFRRMREIMEGR"
     gene            3055..4635
                     /locus_tag="VNU03_24520"
     CDS             3055..4635
                     /locus_tag="VNU03_24520"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77711.1"
                     /translation="MAPDPEPIPPLTGRLRRVGEMLIGLSGSPVPTHLFQTLAEQAET
                     VVPHDYLAVCLEDPEQRGYLVHSLSGLVGEPVGSRVFTRDEGLPGRAIRTGRAHLVGE
                     LERIADGAPDLDGVLVRAGLQAALAVPLRRGMQVLGALLFVRRSAPYTLDDLEVATLL
                     AAGVSGALETCRAYQALADERGTLAAVLTSTADAVVMVGQDGLVLLANPAARAMLGVA
                     PEAMTGRPFFQAIEHEPLRRLFEIGQTGVAELPLPDGRIAQASLVFVSTEYGEPVGLA
                     AVLRDITLLKNLEQMKNDFVNTVSHDLKSPIMAIAMTAELLMKTVPTTGEQAYRDRCQ
                     RILRSANQMTELVTDLLDLGKIEAGLEGPGEPVDLVALIGEVVKAQTVPAEAKGLAIE
                     VRTPPAALVTGVRARLAQVLANLIGNAVKYTRDGGQVQVLVEGAGASDDEPVRVRITD
                     NGIGIPARDLPHVFDKFYRVKNASTAGISGTGLGLAITRSIVETHRGRIGVESTEGSG
                     STFWVELPAAPAGADRPA"
     gene            complement(4643..>5108)
                     /locus_tag="VNU03_24525"
     CDS             complement(4643..>5108)
                     /locus_tag="VNU03_24525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77712.1"
                     /translation="LDFNDVVRAALQATRPCCAIGEGADAHHVEVHFDHGELPAVAGE
                     ASVLIEAVTVVLMNAIEALLGGGVITVRTWAAGAHVVCTIQDDGVGIAPDDQRRALEP
                     FFTTKAGEHKGLGLNVAFGLLRRHGGDLEVAAAPERGTVVTLRLPVIPSSGD"
BASE COUNT          747 a         1876 c         1776 g          709 t
ORIGIN      
        1 cgcttcacgg ggacatggcg acagtcgacg cgcccgaccg cgtggtgggc gaggcggtgg
       61 cgcgcttcgg cgggctcgac gggctggtga gcaacgcggg ggtgaaccgg cccgggccgc
      121 tcgtgagcta cgcggtggag gactgggacc ggatgttcgc ggtgaacacg cgcgcgacgt
      181 ggctcctcgc caaggcggcc cacgacgcgc tcgcggcggc gaagggcgcg atcgtggcgg
      241 tgggatccat gtcgggcagc aacgcccacg ccaacctcgg gccctacggg ccgagcaaag
      301 ccgcggtgat catgctcgtc tcggtgctcg cgcaggagtt cggccgcgac ggcatccgcg
      361 tcaacagcct ctcgcccggc atggtgcgga ccggcatgac cgagaaggtc tacgccgacc
      421 agaccgtggc gagcgcgcgc gacgcgctgg tgccgatcgg ccgcgtcgct acccccgagg
      481 acatggccga cgtgatcgcg ttcctcctcg gccccgacgc acgctacgtc aacggccacg
      541 atctcgtggt cgacggtggc gtgaccggca acttcctcgg ccgcctgccc gggatcggcc
      601 agatcacccg cagctgatcg aactgtcagc gggctgactt ggcccgcctg acagtggccc
      661 ggaggtggcg tgacagccct ccgacggccg ccaagtagct gaaatcatag gtctggcccg
      721 cctcctcgat tggcaccccc ctggcatagt agggacctca gccggaggtc ctcgaatgct
      781 cgcccgactg atcagaatct ggaaccgcta tcgagacgcc gatcaacgcc agctccccct
      841 gtttcctgac tgggagcggg cgcaatggct gttgcgcttg ccgtcggtgg gcgccgcact
      901 cggcctgatc gtgctgttcg cagtgggcgc aacgtggacc gcggagcccg agcgcatcat
      961 cgcgcgggcg gagccggtgg aggaggtcga ggaagtcgag actcccgagt acgacctcgc
     1021 cgcgataccc gattccatca aggcggccgc cgagcgtcac cgcctgccgg agtcgctgat
     1081 cagcgcggtc atcttcgtcg agtcgggctt cgaccacgcc gcggtctcct ccaagggggc
     1141 ccgcgggctg atgcagctga tgccggccac ctccgcgatg atcggcgtga gcgatccgca
     1201 caaccccgag cagaacatcg acgcgggagc gagccaccta cgcgcgatgc tcgacacgtt
     1261 caagggtgac ctgcccctcg ccctcgccgc ctacaacgcg ggcgagggaa acgtggtccg
     1321 ataccacggc attccgccct atccggagac gcgtcgcttc gtggcccggg tgcttcgccg
     1381 aatgggtgac aagccgggcg cggagcgggt gatggccaag cccgtcccga ccccggtgtg
     1441 gacctctcgg acttcgcggc cggctccggc ggtccggatg gccgtctcgc cgccctcctc
     1501 gtcgcgaccg acgatggtcc cgttcccggc tgcgcgcgcc gagcgcgcct cccacgtcgt
     1561 ctaccaggac gacgacgccc ggccccagat ccggagctgg acgtcgtccc ccccgggccc
     1621 tgccgcgtcg ctcgtgccgg agcccacgca gcccttcgcg gaatcgccgt agccgccgca
     1681 ggccctcgcc tcccgcccgc ctcttgcgct acgatggccg aaccccggcc atgccgtggg
     1741 cgtgaaagga gcgggcatgc gattcggggt cttctacgag caccagctgc cccggccgtg
     1801 ggccgcgggt agcgagcaac agctcttcca ggacgcgctg gcccaggtcg agctggccga
     1861 tcggctgggc atcgactacg cctgggaggt ggagcatcac ttcctggagg aatactccca
     1921 ctcctcggcg cccgaggtct tcctggccgc cgcgtcgcag cgcacccggc ggatccgtct
     1981 cgggcacggc atcgtcctga tgccgcccgg ctacaaccat cccgcccgcg tcgccgagcg
     2041 tctggccacc ctcgacctcg tctccaacgg gcgcgtggac tggggcaccg gcgagtcggc
     2101 ctcgcgcgcg gagctggagg gcttcggggt cgagtcggcc gagcgccgcg cgatgtggcg
     2161 ggagaccacc gagcaggtcg ccaacatgct cgcgctggat ccctaccccg gcttccaggg
     2221 caagtacttc tcgatgccga cccgcaacgt ggtgccgaag ccggtgcaga agccgcaccc
     2281 gccgctctgg gtcgcatgct cgaaccggga gaccatccac ctggccgccc agctcggcat
     2341 gggcgcgctc accttcgcct tcatcgaccc ggccgaggcg cgccactggg tcgacgacta
     2401 ctacgagacg ttcaagcgcg agtgcgtccc gatcggacac gcggtcaacc cgaacgtggc
     2461 gatggtgacc gggttctcct gccatcccga cgctcaggag gcgcgccggc gcgggatcga
     2521 cggcttccgc ttcttccagt tcgcgctcgg ccaccactac gtcttcggca agcaccagcc
     2581 cggccgcacc gacatctggg acaagttcga gcgggtgcgg gccgagctcg acctcgaagt
     2641 cctgggcggc ggcaccggct gcatcgggac ccccgacgag ctgcgcgcct ccctccgcgc
     2701 cttcgaggag tatggcgtcg accagaccat cttcatccag cagggcggca agaaccggca
     2761 cgagcacatc tgcgagtcgc tcgagctgtt cgccggcgcg gtgatgcccg agttcaaggc
     2821 gcgcgaggcg gagcgcgcgg cccgcaaggc ggaggcgctc gcgccctacg tggcggcggc
     2881 cttcgcgcgc aagcaggcga tggcccctct cgccgacgcc gacatcccgg cctacgaggc
     2941 ctacggcctc accgtgaccc agccggtcga cctcgacaag atgccggagg ccaaccggcg
     3001 ccgggttctg actttccgaa ggatgcggga gatcatggag ggccgctagg ctccatggcg
     3061 ccggacccgg agccgatccc gccgctgacc ggccggctca ggcgcgtcgg ggaaatgctc
     3121 atcgggctct cgggctcgcc cgtccccacg catctcttcc agacgctggc cgagcaggcc
     3181 gagacggtcg tgccgcacga ctacctggcg gtctgcctgg aagaccccga gcagcgcggc
     3241 tacctcgtgc attcgctatc cggtctggtc ggcgagccgg tgggcagccg ggtgttcacg
     3301 cgggacgagg ggctgcccgg gcgcgcgatc cgcaccgggc gcgcgcacct cgtcggcgag
     3361 ctcgagcgta tcgccgacgg cgcgcccgat ctcgacggcg tgctcgtgcg ggcggggctc
     3421 caggcggcgc tcgcggtccc gctgcgccgc gggatgcagg tgctgggcgc gctcctgttc
     3481 gtgcgccgga gcgcgcccta caccctcgac gacctcgagg tcgcgacgct gctcgcggcc
     3541 ggagtctcgg gcgcgctcga gacctgccgc gcgtaccagg ccctggccga cgagcgcggc
     3601 accctggccg cggtcctcac cagcaccgca gacgcggtgg tgatggtcgg acaggacggc
     3661 ctcgtgctcc tcgccaaccc ggccgcgcgc gccatgctgg gggtcgcgcc ggaagccatg
     3721 accgggcggc cgttcttcca ggccatcgag cacgagccgc tccggcggct cttcgagatc
     3781 gggcagaccg gcgtcgccga gctgccgctg cccgacgggc gcatcgccca ggcgagcctc
     3841 gtcttcgtct ccaccgagta cggcgagccg gtggggctcg ccgcggtgct gcgcgacatc
     3901 acgctgctca agaatctcga gcagatgaag aacgacttcg tgaacacggt gtcccacgac
     3961 ctcaagagcc cgatcatggc catcgcgatg accgccgagc tgctcatgaa gacggtgccc
     4021 accaccggcg agcaggccta ccgcgaccgc tgccagcgca tcctccgcag cgcgaatcag
     4081 atgaccgagc tggtgaccga cctgctcgac ctgggcaaga tcgaggccgg tctcgagggc
     4141 ccgggcgagc cggtcgacct ggtcgccctg atcggcgagg tggtgaaggc gcagaccgtc
     4201 cccgcggagg ccaagggcct cgccatcgag gtgcggacgc cgcccgccgc gctcgtcacc
     4261 ggcgtgcggg cgcggctcgc ccaggtgctg gccaacctga tcggcaacgc ggtgaagtac
     4321 acgcgggacg gcgggcaggt ccaggtcctg gtcgaggggg cgggcgcctc ggacgacgaa
     4381 ccggtccggg tgcgcatcac cgacaacggg atcggcatcc ccgcccgcga cctgccgcac
     4441 gtcttcgaca agttctaccg ggtcaagaac gcctccaccg ccggcatctc gggcaccggc
     4501 ctcggcctgg ccatcacccg cagcatcgtg gagacccacc gcgggcggat cggcgtggag
     4561 agcacggagg gatccggcag cacgttctgg gtcgagctgc ccgcggcgcc ggccggcgcc
     4621 gaccgccccg cgtgacgcgg agctagtctc ccgaggacgg gatcacgggc aggcggaggg
     4681 tcaccacggt gccccgctcc ggggcagccg cgacctcgag atcccctccg tggcggcgca
     4741 gcaggccgaa ggcgacgttc agacccaggc ccttgtgctc gccggccttg gtggtgaaga
     4801 agggctcgag cgcccggcgc tggtcgtccg gcgcgatgcc gacgccgtcg tcctggatgg
     4861 tgcagaccac gtgcgccccc gcggcccagg tcctgaccgt gatgacgccg ccccccagga
     4921 gcgcttcgat cgcgttcatg agcacaaccg tgaccgcctc gatcagcacg gaggcctcac
     4981 ccgccaccgc gggcagctct ccgtggtcga agtgcacttc gacgtggtgc gcgtccgccc
     5041 cttccccgat cgcgcagcag gggcgagtag cctgaagcgc cgcccgcacc acgtcgttga
     5101 agtcgagc
//
LOCUS       DATMCV010001069         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_595356, whole genome shotgun sequence.
ACCESSION   DATMCV010001069 DATMCV010000000
VERSION     DATMCV010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595356"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VNU03_24530"
     CDS             <1..303
                     /locus_tag="VNU03_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77713.1"
                     /translation="RDRIRQLLSEIMTDELAHVGQRRNFMGPIGLRAARRFVEPMYRA
                     FFRDIPETRCLFDLARMVQDGKTFDYSTIAPGMIERSWVPSYCQAVEPGHAGGHLW"
     gene            318..1730
                     /locus_tag="VNU03_24535"
     CDS             318..1730
                     /locus_tag="VNU03_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWP77714.1"
                     /translation="MNGIVGYGAYLPYFRLDRKAIGDALGSGGGKGTRVVASYDEDTT
                     SMGVEAARTALRGAPRLAAAAVYFATAEPAYLDKTNATAIHAALALDESAMAFDMLGS
                     IRSAAGALRAAMDAGRPTLAVLADIRTGLPGGADERDGGDAAAAFLLAAGDADTPVLV
                     EPVAAASVTAEFLERWRVPGDAASRQWEERFGEHAYVPLAEAAIASALEQAGVTAGDL
                     ASVLVAGSHGRANKRAAAAVGAKPTALGDDLTGTVGNTGAAHAGLLLADALDRAQADD
                     LIMVVSLADGCDVTIWRATAALARARQRVPVAQQIKAGGKVAYATFLTWRGFLKREPP
                     RRPDPQAPAAPPAFRAEAWKFGFTGSRCQTCGTRHLPPTRVCLKCRAVDRMTPERMAD
                     VPATIATYTVDRLAYSLSPPVVVGVLDFEGGGRFTCGLTDVDPAAVKIGDRVEMTFRR
                     LMTAHGVHNYFWKCRPAGTT"
BASE COUNT          362 a          864 c          868 g          367 t
ORIGIN      
        1 cgtgaccgga tccggcagct gctgagcgag atcatgaccg acgagctggc ccacgtcggc
       61 cagcgccgca acttcatggg tccgatcggg ctccgcgcgg cccgccggtt cgtggagccc
      121 atgtaccggg cgttcttccg cgacatcccc gaaaccagat gcctgttcga cctcgcgcgc
      181 atggtccagg acgggaagac attcgactac agcacgatcg cgcccggcat gatcgagcgg
      241 agctgggttc cctcgtattg ccaggcggtc gagcccgggc acgcgggcgg ccacctgtgg
      301 tagcttcgac cccacccatg aacggcatcg tcggctacgg cgcctatctc ccgtacttcc
      361 gcctcgaccg gaaggcgatc ggcgacgccc tcggcagcgg gggcggcaag gggacccggg
      421 tcgtggcctc ctacgacgag gacacgacct ccatgggcgt ggaggcggcg cgcacggccc
      481 tgcgcggcgc cccgcggctc gctgccgccg cggtgtactt cgcgaccgcc gagcccgcgt
      541 atctcgacaa gaccaacgcc accgccatcc atgccgcgct cgccctcgac gagtcggcga
      601 tggccttcga catgctcggc tcgatccgct cggcggcggg ggcgctgcgc gccgcgatgg
      661 acgccgggcg gccgacgctg gcggtgctgg cggacatccg caccgggctg ccgggcggcg
      721 cggacgagcg cgacggcggc gacgcggcgg ccgccttcct gctcgcggcg ggtgacgcgg
      781 acacgccggt gctggtcgag ccggtggccg cggcgtcggt caccgccgag ttcctggagc
      841 gctggcgggt gccgggcgat gcggcctcgc ggcagtggga ggagcgcttc ggcgagcacg
      901 cttacgtgcc gctcgcggag gccgccatcg cgagcgcgct cgagcaggcc ggcgtcaccg
      961 ccggcgacct cgcgtccgta ctggtggcgg ggtcgcacgg gcgcgcgaac aagcgcgcgg
     1021 ccgccgcggt gggcgcgaag cccaccgcgc tcggcgacga tctcaccggc accgtcggca
     1081 acaccggcgc ggcccacgcg gggctgctgc tcgcggacgc gctcgaccgg gcgcaagccg
     1141 acgacctcat catggtggtc tcgctggccg acggctgcga cgtgacgatc tggcgggcga
     1201 ccgcagcgct ggcgcgcgcc cggcagcggg taccggtggc gcagcagatc aaggcgggcg
     1261 ggaaggtggc ctacgccacc ttcctcacct ggcgcggctt cctcaagcgt gagccgccgc
     1321 gccggcccga tccgcaggcc cccgcggcgc cgcccgcctt ccgggccgag gcgtggaagt
     1381 tcggcttcac cgggagccgg tgccagacgt gcggcacgcg gcacctgccg cccacgcggg
     1441 tgtgcctcaa gtgccgcgcg gtggaccgca tgacgcccga gcggatggcg gacgtgcccg
     1501 cgaccatcgc gacctacacg gtggaccggc tggcctactc gctctcgccg ccggtggtgg
     1561 tgggcgtgct cgacttcgag ggtggcggcc gtttcacctg cgggctgacc gatgtggatc
     1621 ccgccgcggt gaagatcggc gaccgggtcg agatgacgtt ccgccggctc atgaccgctc
     1681 acggggtgca caactacttc tggaagtgca gaccggccgg aaccacctga gtgtgctgag
     1741 cgttttgcct cggattggtt gactcccccg ggggagcaaa atcactcggc aatcttccgg
     1801 gtccgctgga aggaaagctg tcaacgcggg tcaggtcctg tctagttctc gaacaccagc
     1861 agcgccgacg gtggcccgcc attggcatgg cgagagctct cgcacggacg atgagcgagg
     1921 acgaagtctc ggacttctgc gtacacaccc atcgcctcgt ctccttgtca aaatcccgat
     1981 gaggctagag cccgagcgcg tcgatctggg ccggcgtcag cgcgacccgc gggaccgagt
     2041 agatccgacg cttcgccaga ctctgccacg cccgttcaat ctccggaaga ggaacgtggg
     2101 cttgtcgaag gaagtctgtg agtgcgtcca agccctggca ctgggcatgt cgggtacgaa
     2161 gccgcccgcc cagcggggcg aagacgatcc ggtactcggc gttgacgggg tcgattggac
     2221 cagggcctcg ggtgatatgc agaaacccca aggccggatt ttccatggca cccaatcata
     2281 ggggcgcccc gcgaccggac catcatggag acttcgacga accgctgcca gctcttgtgg
     2341 tcatcactca gcgaccttta cctcgaaggt caccgtgttg tcccccccgg cgttcttgat
     2401 gacgaacttg tacttcccgt ccttcgccgg cgtgactttc accgaacact tcgggccggg
     2461 c
//
LOCUS       DATMCV010001070         2023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_463621, whole genome shotgun sequence.
ACCESSION   DATMCV010001070 DATMCV010000000
VERSION     DATMCV010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463621"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VNU03_24540"
     CDS             complement(<1..1430)
                     /locus_tag="VNU03_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWP77715.1"
                     /translation="MEMSILKRLIVGAPMPLAQARHERLSKTVALAVFSSDAMSSVAY
                     ATEEILLILVLAGTAAAHLTVPIALAIAGLLVVVSVSYQQTIHAYPSGGGSYIVARAN
                     LGPTAGLVAAAALLIDYVLTVSVSVAAGVAALTSAVPWLLTHRVLLGVVFTGAIAYAN
                     LRGVRESGRVFAVPTYLFIVTFSVLVGTGLFRWLTGTLPAAAAASETVAATQTLTWFL
                     VLRAFASGCTALTGVEAISNGVPAFKHPEARNAAITMGFMAAVLGTLFIGVSVLASAL
                     AITPLADETVVSQVARRLFGDGFFYFLIQGSTTLILVLAANTSFADFPRLNSLLARDR
                     YAPRQFRTLGDRLVFSNGILILAGLAAALIVVFGGDTHALIPLYAVGVFISFTLSQAG
                     MVRHWLTDGGAGWRWRLGVNGVGALVTGAVTVVIAVTKFTHGAWIVVLLIPLLVLGFR
                     AIYRHYDTVAHELSLEHLVEEPPVNNT"
     gene            1494..>2023
                     /locus_tag="VNU03_24545"
     CDS             1494..>2023
                     /locus_tag="VNU03_24545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77716.1"
                     /translation="MIARRFGSTLALLAVLSAVASSTPAAAQSDPTRTRLAAPVKVKQ
                     GMANVPALSPLWLLPEEAAKYNVQVEQVIFQRFADARTALASGDLDIAAFGPQDIILG
                     LGQGAKSMIGVAGVGSGNDCLLVRKGEDIKDWAGIAPKRIGIGAGSISWLKFAAAVQE
                     NGLDYGKLRIVNIMGGG"
BASE COUNT          348 a          667 c          752 g          256 t
ORIGIN      
        1 gtgttgttca ccggcggctc ctcgacgaga tgctcgaggg agagctcgtg ggccaccgtg
       61 tcgtagtgcc ggtagatcgc gcggaacccc aggaccagga gggggatcag gaggacgacg
      121 atccacgccc cgtgggtgaa cttggtcacc gcgatgacca ccgtcaccgc cccggtcacg
      181 agcgcgccga ccccgttcac gcccaggcgc caccgccagc ccgcgccgcc gtcggtgagc
      241 cagtgccgga ccatgccggc ctgcgagagc gtgaacgaga tgaagacccc gaccgcgtag
      301 agcgggatca gggcgtgggt gtcgccgccg aagacgacga tgagcgcggc ggccaggccg
      361 gccaggatca ggatgccgtt ggagaagacc agccggtcgc ccagggtccg gaactgccgg
      421 ggcgcgtagc gatcgcgggc gagcagcgag ttcaggcgcg ggaagtcggc gaatgaggtg
      481 ttggcggcca gcaccaggat gagggtggtc gagccctgga tcaggaagta gaagaacccg
      541 tcgccgaaga gccgtcgggc cacctgggag acgacggtct cgtcggccag cggcgtgatc
      601 gcgagcgcgg acgccagcac cgagacgccg atgaagagcg tgccgaggac cgcggccatg
      661 aagcccatgg tgatggccgc gttgcgggcc tccggatgct tgaaggcggg gacgccgttc
      721 gagatcgcct ccaccccggt gagcgcggtg cagccggacg cgaaggcgcg cagcacgagg
      781 aaccaggtca gggtctgggt ggccgcgacc gtctcggagg ccgcggccgc cgcgggcagg
      841 gtgccggtga gccagcggaa gaggccggtg cccaccagca cgctgaaggt gacgatgaag
      901 agataggtcg ggaccgcgaa cacccggccg gactcgcgga cgccgcgcag gttggcgtag
      961 gcgatggccc cggtgaacac gacgccgagc agcacgcggt gggtcaggag ccacggcacc
     1021 gcggaggtga gcgcggccac ccccgccgcg accgataccg acacggtcaa tacgtagtcg
     1081 atgagcagcg ccgccgccgc gacgaggccc gcggtcggtc cgaggttggc gcgggccacg
     1141 atgtaggagc cgccgccgct cgggtaggcg tggatggtct gctggtacga cacgctcacc
     1201 accaccagga gccccgcgat cgcgagcgcg atcggcaccg tgaggtgggc cgccgcggtg
     1261 ccggcgagga ccaggatcag gaggatctcc tccgtcgcgt acgcgaccga cgacatcgcg
     1321 tcggaggaga agaccgcgag agcgacggtc ttggaaagcc gctcgtgccg cgcctgcgcg
     1381 agaggcatgg gcgcgcccac gatgaggcgc ttcagaatgc tcatttccat gcttgcctaa
     1441 ggctgctccc ttcgcgcatt ctacgcaacg tgctcgcgaa aggagcccaa cgaatgatcg
     1501 cccgacgctt cggctcgacg ctcgcgctgc tcgccgtgct ctccgccgtc gcgtcgtcga
     1561 ctccggccgc ggcccagtcg gaccccacgc ggacccgtct ggccgccccg gtcaaagtga
     1621 agcaggggat ggcgaacgtg cccgccctgt cgccgctgtg gctcctgccc gaggaggccg
     1681 cgaagtacaa cgtccaggtc gagcaggtca tcttccagcg cttcgcggac gcgcgcaccg
     1741 cgctcgcctc gggcgacctc gatatcgccg cgttcggtcc gcaggacatc atcctgggcc
     1801 tcggccaggg cgcgaagtcg atgatcgggg tggccggggt cggctccggc aacgactgcc
     1861 tgctggttcg caaaggcgag gacatcaagg actgggccgg gatcgccccg aagcggatcg
     1921 ggatcggggc cggctccatc tcgtggctca agttcgcggc cgcggttcag gagaacggcc
     1981 tcgactacgg caagctccgc atcgtgaaca tcatgggagg cgg
//
LOCUS       DATMCV010001071         2515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_56210, whole genome shotgun sequence.
ACCESSION   DATMCV010001071 DATMCV010000000
VERSION     DATMCV010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56210"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VNU03_24550"
     CDS             <1..768
                     /locus_tag="VNU03_24550"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWP77717.1"
                     /translation="LALAPIADDEPLAISHRQVDDYETCPLKYQYIHILRIPLRQHHS
                     VVYGSALHNAVEFYLRRRAAGNYTSLEDFLRAFDNAWRNEGFLTREHEEQRKRAGVAA
                     LTRFYHEEEASGQKPTDVEREFGFNLGLNRVRGRFDRVDETPEGLVIVDYKSSDVTDQ
                     KKADQRARESLQLKIYALAQHEMTGRLPARVELRFLESGLTGRHTPTLDDLMTARDAI
                     EAAARGIRQREFEATPDYQACRYCPYNQICPSTATQE"
     gene            817..1617
                     /gene="pcaD"
                     /locus_tag="VNU03_24555"
     CDS             817..1617
                     /gene="pcaD"
                     /locus_tag="VNU03_24555"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HWP77718.1"
                     /translation="MKLSANGIDINYEIEGDGPVVTFSHSLACNLSMWDEQVRALRSR
                     YRVLRYDTRGHGQTGAPAGAYTLDQLADDLKGLLDGLGIAATHFVGLSMGGMIGQVFA
                     LKHPAMVQSLVLCDTTSRYPQGAAAVWEERIKTVGAKGMEPMVAPTLERWFTAAFRSR
                     RKDLMEKVGAMIRSTPAAGYVGCCHALPKINVTERLRDVRCPALVIVGEEDPGTPVDM
                     ARDIHAALPVAELAVLCRASHLSNVEQPGEFNRALGGFLDKVSGRTKL"
     gene            1695..>2515
                     /locus_tag="VNU03_24560"
     CDS             1695..>2515
                     /locus_tag="VNU03_24560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP77719.1"
                     /translation="MLFTYLTTHFLNHALGLISIDAMLAGREWFVLLWRSYLGTLALY
                     TALVGHLSLAYWSLYRRRTLRMPGWEALQLSLGLTIPLLIVWHIVGTRLAHEVAGTQD
                     TYTLVVLNLWKLDPVAGLRQATALVVAWLHGCVGLHFWLRLRPWYARYWRTIYSGFLL
                     LPVLALLGFAEAGREITRLAEDPAFVPRVLAAAHLPSPDQRVVLGRVSHGIFWGHLGM
                     IALVLAVRAGREHVRRRQGVRVTYPGGREVLVPAGFTILEASRFGGIRHASVCGGR"
BASE COUNT          360 a          927 c          861 g          367 t
ORIGIN      
        1 ctcgccctcg ccccgatcgc ggacgacgag ccgctcgcga tcagccaccg gcaggtggac
       61 gactacgaga cctgcccgct caagtaccag tacatccaca tcctgcgcat cccgctccgg
      121 cagcaccact cggtcgtcta cggcagcgcg ctccacaacg cggtcgagtt ctacctgcgc
      181 cgccgcgcgg cgggcaacta cacctcgctc gaggacttcc tgcgcgcgtt cgacaacgcg
      241 tggcggaacg agggctttct cacccgcgag cacgaggagc agcgcaagcg ggccggggtg
      301 gccgcgctga cccgcttcta ccacgaggag gaggcctccg ggcagaagcc caccgacgtg
      361 gagcgcgagt tcgggttcaa cctcggcctc aaccgggtcc gcgggcgctt cgaccgggtc
      421 gacgagacgc ccgaggggct cgtgatcgtc gactacaagt cgagcgacgt caccgaccag
      481 aagaaggcgg accagcgcgc gagggagagc ctccagctca agatctacgc gctggcccag
      541 cacgagatga ccgggcggct gcccgcgcgg gtggagctgc gcttcctcga gtccggcctc
      601 acagggcggc acacgcccac gctggacgac ctgatgaccg cgcgggacgc catcgaggcg
      661 gcggcgcgcg gcatccggca gcgggagttc gaggcgacgc ccgactatca ggcctgccgc
      721 tattgcccgt acaatcagat ctgccccagc accgcgaccc aggagtgaac cccagacacc
      781 ctctcgcgac cggggagagg gcagggtgag ggcaagatga agctcagcgc caacggcatc
      841 gacatcaact acgagatcga gggcgacggc ccggtggtga ccttcagcca ctcgctggcc
      901 tgcaacctct ccatgtggga cgagcaggtg cgcgccctcc ggagccgcta ccgcgtgctg
      961 cgctacgaca cgcgcggcca cgggcagacc ggcgcgcccg ccggcgccta cactctcgac
     1021 cagctcgcgg acgacctgaa gggcctgctc gacgggctcg gcatcgcggc cacccacttc
     1081 gtcgggctct cgatgggcgg catgatcggg caggtcttcg cgctgaagca cccggccatg
     1141 gtccagagcc tcgtgctctg cgacaccacg agccgctacc ctcagggcgc ggccgcggtg
     1201 tgggaagagc gcatcaagac ggtgggggcc aagggcatgg agccgatggt cgcccccacc
     1261 ctcgagcggt ggttcaccgc cgcgttccgg tcgcgccgga aggacctgat ggagaaggtc
     1321 ggcgccatga tccgcagcac gcccgcggcg ggctacgtcg gctgctgcca cgcgctgccc
     1381 aagatcaacg tcaccgagcg cctccgcgac gtccgctgcc ccgccctcgt gatcgtcggc
     1441 gaggaggacc ccggcacgcc ggtcgacatg gcgcgcgaca tccacgcggc cctgccggtg
     1501 gccgagctcg cggtgctgtg ccgcgcgtcg cacctgtcga acgtcgagca gcccggcgag
     1561 ttcaaccgcg cgctcggcgg tttcctcgac aaggtgagcg ggcggacgaa gctgtagcga
     1621 cgccgccccg ccccatgccc ccggcccgat cagcggcggc cgtcgtccga cagacccgcc
     1681 tctggttggg cctcgtgctg ttcacgtacc tcaccactca cttcctgaat cacgcgctcg
     1741 gcctgatctc gatcgacgcg atgctggccg ggcgcgagtg gttcgtcctg ctctggcgca
     1801 gctacctggg gacgctcgcg ctctacaccg cgctcgtcgg ccatctctcg ctcgcctact
     1861 ggtcgctcta ccgccggcgt accctgcgca tgccggggtg ggaggcgctg cagctctcgc
     1921 tcggcctgac catcccgctg ctgatcgtct ggcacatcgt cggcacgcgg ctggcgcacg
     1981 aggtggcggg gacgcaggac acctacaccc tcgtggtgct caacctctgg aagctcgatc
     2041 cggtggcggg gctccggcag gccaccgcgc tcgtcgtcgc gtggctgcac gggtgcgtgg
     2101 gcctccactt ctggctgcgc ctgcggccgt ggtacgcgcg ctactggcgc acgatctaca
     2161 gcgggttcct gctccttccg gtgctggccc tgctgggctt cgcggaggcg ggccgcgaga
     2221 tcacccgcct cgccgaggac cccgcgtttg ttccgcgcgt gctcgcggcc gcgcacctgc
     2281 ccagtcccga tcagcgcgtg gtgctcgggc gggtctcgca cgggatcttc tggggccatc
     2341 tcgggatgat cgcgctggtc ctcgcggtgc gtgccgggcg cgagcacgtc cgccgtcggc
     2401 agggcgtgcg ggtgacctat cccggcgggc gcgaggtgct ggtccccgcc ggcttcacca
     2461 ttctcgaggc gagccgcttc ggcgggatcc gccacgcctc cgtctgcggc gggcg
//
LOCUS       DATMCV010001072         3448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_791861, whole genome shotgun sequence.
ACCESSION   DATMCV010001072 DATMCV010000000
VERSION     DATMCV010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791861"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..974)
                     /locus_tag="VNU03_24565"
     CDS             complement(219..974)
                     /locus_tag="VNU03_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77720.1"
                     /translation="MSAPARVAVVTGAARGIGRATAAALAKSGFRVGAVDVRGPELRE
                     TAALVREAGPEALALEGDVTSLATAQRLAGEVLAAWGRVDVLVNNAGISQPKGLLEIT
                     EEEWDLTIDTHLKGTFNWCKAVAPAMLAQRAGRIVNMSSVSAHTGAGTAAVSKSAYCA
                     GKAGILGFTRGLAKELAPHVTVNAICPGLIATDLTAGMIAREGAETLVRSIPLARLGT
                     PEDIAQVVVFLATVTPCFLTGEVIDVDGGQWVN"
     gene            complement(971..2788)
                     /locus_tag="VNU03_24570"
     CDS             complement(971..2788)
                     /locus_tag="VNU03_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013385792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWP77721.1"
                     /translation="MSRRRRSTRRPPVPALDPVTREIIRGALRAAQTHMEAVIERTAM
                     SPFIREKNDYFAGILDARGRVVCGTMVPLFGNLTQIIFEQYPPDTMRPGDLYWYNDCH
                     GSHGGVSHSPDMVFAAPVFHRGRLVAFSQTWGHFWDIGGMRAGSISPDATEIFHEGII
                     VPAVRIYREGVLNDEAFRIFLRNSRFPEILRGDIRAVLAGCQLGERRVLELFDRFGAE
                     TVLTAWALFEQQCRDTIRKTLESRIPDGEYESDDAVDGDGMSGRPFHVRMRLTKEGGR
                     ITLDTRASDDQARGPINFIMHPSVPKLIVGIYLLSGHPTVLLNDGAQDAIDEVLVRPG
                     SILQPHWPAALGNRAHTLARVQSGVLALLALATGGDVPAPNSVYNIYFLRGFDRARNE
                     FFLCSDGVAVGYGARPFADGLDAIYYVAQKNYPAEFMEMVFPLRLRRYALHQDSGGPG
                     RFRGGAGVIREIELTTDEAIVAIRQDNILFPPAGVNGGHAGRPGRCVVNPGRSDERPL
                     APMSDGNVLRKGDVLRLSTSGGGGWGHPFDRALERVRRDVLGGVVSIGAAREDYGVVI
                     DARGTIDTAATEALRARPRGPVRMFHRDGYFGPPVESGR"
     gene            complement(2785..>3448)
                     /locus_tag="VNU03_24575"
     CDS             complement(2785..>3448)
                     /locus_tag="VNU03_24575"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWP77722.1"
                     /translation="VLSTFGLLGTEVRNDYARTSLQKPPDFDLGAVAAVYADLEGQAQ
                     AWLAAEGVAPSARRLTRMADLRYRHQGFELTVPWPERDLGLDALLARFHARHRQLYTY
                     ALADAPVEIVTLRVSAAGRVRRFTLPSLARRRAVATRPPRRRVHFPGAGWTACPCVDR
                     ERLGVGAVVTGPAIVEQLDTTTVVPPGHRARVDRVGNLVIQTGRSPSPLRGAGRVRGR
                     TR"
BASE COUNT          484 a         1225 c         1292 g          447 t
ORIGIN      
        1 acgggctctc gttcgcgaag acgaaccggg tctgcgcgag agccggcgcc gcggggatcg
       61 ccgcgagcat gagcacgacg aggacgagga gcagccagga acgaccgtgg gtcatgagca
      121 gcctccagga gtcccgaagt gtcggcggcg tcggcggtgt ggcgggattg tactgcacgg
      181 ccccggggcc gacaagggcc ggccggccga cggcgggctc agttcaccca ctgcccgccg
      241 tcgacgtcga tgacctcgcc ggtcaggaag cacggcgtca cggtggcgag gaagacgacg
      301 acctgggcga tgtcctccgg cgtgccgagc cgcgcgaggg ggatcgagcg cacgagggtc
      361 tcggcaccct cgcgcgcgat catcccggcg gtcaggtcgg tcgcgatcag cccggggcag
      421 atcgcgttca ccgtcacgtg cggggccagc tccttggcca gcccccgggt gaagccgagg
      481 atgccggcct tgcccgcgca gtaggccgac ttgctcaccg ccgcggtgcc ggccccggtg
      541 tgcgcgctca ccgacgacat gttcacgatc ctcccggcgc gctgggccag catcgccggc
      601 gccaccgcct tgcaccagtt gaacgtcccc ttgaggtgcg tgtcgatggt gagatcccac
      661 tcctcctcgg tgatctcgag cagccccttg ggctgcgaga tgccggcgtt gttgacgagg
      721 acgtcgacgc ggccccaggc ggccaggacc tcgccggcca gccgctgcgc cgtcgccagg
      781 ctggtgacat cgccctccag cgcgagcgcc tcgggcccgg cctcgcgcac cagcgccgcc
      841 gtctcccgca gctccgggcc gcggacgtcg acggcgccca cccggaagcc gctcttcgcc
      901 agtgcggccg cggtggcgcg gccgatgcca cgcgcggcgc cggtcaccac cgccacccgg
      961 gccggcgcgc tcatcgcccg gactcgaccg gcgggccgaa gtagccgtcg cggtggaaca
     1021 tgcgtaccgg cccgcgcggc cgcgcgcgca gcgcctcggt ggccgccgtg tcgatcgtcc
     1081 cgcgcgcgtc gatgaccacg ccgtagtcct cccgggccgc gccgatcgac acgacgccgc
     1141 ccaggacgtc gcgccggacg cgctcgagcg cccggtcgaa gggatggccc cagccgccgc
     1201 caccgctggt ggacaggcgc agcacgtcgc ccttgcgcag cacgttgccg tcggacatcg
     1261 gcgcgagcgg ccgctcgtca gatcggcccg ggttcaccac gcagcgcccg ggccgaccgg
     1321 cgtggccgcc gttcacgccg gcgggcggga agaggatgtt gtcctggcgg atggccacga
     1381 tcgcctcgtc ggtggtcagc tcgatctcgc ggatcacgcc cgctcccccg cggaagcgcc
     1441 cggggccgcc cgagtcctgg tgcagcgcat agcgacggag acgcaggggg aagaccatct
     1501 ccatgaactc ggccgggtag ttcttctggg ccacgtagta gatggcgtcg aggccgtccg
     1561 cgaacggccg cgcgccgtag cccaccgcga cgccgtcgct gcagaggaag aactcgttcc
     1621 gggcccggtc gaagccgcgc aggaaataga tgttgtagac cgagttcggc gccggcacgt
     1681 cgcccccggt ggcgagggcc aggagagcga ggacgccgga ctgcacgcgg gcgagggtgt
     1741 gcgcccggtt gccgagtgcc gcgggccagt gcggctgcag gatgctgccc gggcgcacga
     1801 ggacctcgtc gatggcgtcc tgggcgccgt cgttgagcag cacggtcgga tgccccgaga
     1861 gcaggtagat gcccacgatg agcttcggca cgctcgggtg catgatgaag ttgatcgggc
     1921 cgcgggcctg atcgtcgctc gcgcgggtgt cgagcgtgat ccggccgccc tccttggtga
     1981 ggcgcatccg cacgtggaac ggtcgcccgc tcatcccgtc gccgtccacc gcgtcgtcgg
     2041 actcgtattc gccgtcggga atccgggact ccagcgtctt gcggatcgtg tcgcggcact
     2101 gctgctcgaa cagcgcccac gcggtcagca ccgtctcggc gccgaagcgg tcgaacagct
     2161 cgaggacccg ccgctcgccg agctggcagc ccgccagcac cgcgcggatg tcgcccctga
     2221 ggatctccgg gaagcgcgag ttgcgcagga agatgcggaa cgcctcgtcg ttcagcacgc
     2281 cctcgcggta gatgcggacc gcggggacga tgatgccctc gtggaagatc tcggtggcgt
     2341 ccggcgagat gctgcccgcc cgcatgccgc cgatgtccca gaagtggccc caggtctgcg
     2401 agaaggccac gagccggccg cggtggaaca ccggcgccgc gaagaccatg tcgggcgagt
     2461 gcgagacgcc cccgtgggaa ccgtggcagt cgttgtacca gtagaggtcg ccgggccgca
     2521 tcgtgtcggg tgggtactgc tcgaagatga tctgggtgag attgccgaag agcgggacca
     2581 tggtgccgca gaccacgcgg ccccgcgcgt cgaggatgcc ggcgaagtag tcgttcttct
     2641 cgcggatgaa cggcgacatc gcggtgcgct cgatgaccgc ttccatgtgc gtctgcgcgg
     2701 cccggagggc gccgcggatg atctcgcgcg tcaccgggtc gagcgcgggc acgggaggcc
     2761 ggcgcgtgga ccgccgccgt cggctcatcg cgtgcgcccc ctcacccggc ccgctccccg
     2821 taggggagag ggagagcggc cggtctggat gacgaggttg ccgacgcggt ccacccgggc
     2881 ccggtgcccg ggcggtacca cggtcgtggt gtcgagctgc tcgacgatgg cggggcccgt
     2941 caccaccgcg cccaccccca ggcgctcgcg atcgacacag gggcacgcgg tccagcccgc
     3001 gcccggaaag tggacgcgcc gccgcggagg gcgcgtcgcg accgcgcggc ggcgcgcgag
     3061 ggagggcagc gtgaagcgcc ggacccgccc ggccgcggac acccgcagcg tgacgatctc
     3121 gaccggcgcg tcggccagtg cgtacgtgta gagctgacgg tggcgggcgt ggaaccgcgc
     3181 gaggagcgcg tccaggccga gatcgcgctc gggccacggc acggtgagct cgaaaccctg
     3241 gtggcggtag cggaggtcgg ccatccgcgt gagccggcgc gcggacgggg ccacgccctc
     3301 cgcggccagc caggcctgcg cctggccctc gagatcggcg tacacggcgg cgaccgcgcc
     3361 gaggtcgaaa tcgggcggct tctgcaggct cgtccgggcg tagtcgttgc ggacctcggt
     3421 gccgaggaga ccgaacgtcg acagtacc
//
LOCUS       DATMCV010001073         4518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_791980, whole genome shotgun sequence.
ACCESSION   DATMCV010001073 DATMCV010000000
VERSION     DATMCV010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791980"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VNU03_24580"
     CDS             complement(<1..258)
                     /locus_tag="VNU03_24580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77723.1"
                     /translation="MTRLAAACLMGWAATTNYTNHAAVLPLLMAELGFGPVQAGLLST
                     AFFVTLAVGCVPAGLLIDRLGPTGVGTAGLIAVFASNLALGS"
     gene            complement(263..1279)
                     /locus_tag="VNU03_24585"
     CDS             complement(263..1279)
                     /locus_tag="VNU03_24585"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP77724.1"
                     /translation="MCFIDTDGMGARSPLSRRGFLGGLVAGAAAAASLGGSLVLPGVA
                     AGAEPAPAGGGPGAAGVSFKWFGTNGWEITFGNKTILVDPWFNRFESGFLQNKLDHDR
                     LLPTDTALIDQHVKKADQILIGHGHWDHMADVPYIAKKTGAMVVGSETHANVMRAAGV
                     PEGKIVQVKGGEYMQFDGYSIEVFPSVHSMGGTKKYAVPGHLVSVPASPPTKVGDLPE
                     GDTLVYVLRIAGKYTIFLMSSGNYIERAIAGLKPDVALIAPLFSNNTVDFTPRLVRAL
                     GYPKLVLPTHWDNFEAPLTSTPTDLRTVYGDPANLDLWVKEMRKVTPRTRIVTMPYFG
                     SYAP"
     gene            complement(1293..2306)
                     /locus_tag="VNU03_24590"
     CDS             complement(1293..2306)
                     /locus_tag="VNU03_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP77725.1"
                     /translation="MDDPRGVSRRALLLSAGLGLGTVTVDGARKPLLAQADRPRADEL
                     WSAGYVARKGDVSLALYRKRIGPPQAGERPVLFLVHGSSVSARPSFDLTVPGRGEYSL
                     MNVFAGYGFDVWTMDHEGYGKSARTAGNSDVASGVEDLKAASEVVVRMTGQPRAHFYG
                     GSSGALRAAGFAMARPERVDRLILEAFTWTGKGSPTLGKRAEQVDFYRTHNRRPRGRD
                     MIRSIFTRDKPGTSDPAVAEALADAELPFGDTIPTGTYLDMTANLPVVDPRRVKAPVL
                     MVRGEHDGIATEDDLLEFFRRLPNPDRQFIVLAGASHAVALGLSRGQLWHVVRAFLTL
                     PTA"
     gene            complement(2332..3558)
                     /locus_tag="VNU03_24595"
     CDS             complement(2332..3558)
                     /locus_tag="VNU03_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HWP77726.1"
                     /translation="MLSLAVLSSLVGALLGAALPASGQELPEGPGKELVAAQCNSCHP
                     LTSRVGAGYTPAGWRTVMRMMINNGVPIPPDQLATMTAYLVKTFPEKPKPVGVVIPGP
                     VKVSFKEWPVPTPGSRPHDPLATADGALWYTGQMANVLGRLDPKTGRFKEYPLPTPHG
                     GPHGLVADQAGNIWYTANAGGLIGKLEPKTGAVTEYKMPDPAVQDPHTLIFDRSGILW
                     FTAQNANRVGRLDPKTGEITLVTPPTPKSRPYGMAVSSKGAVFYVAFGSNRVGSVDPM
                     TMEIREYPLPDAASRPRRITITRDDVVWYTDFSRGYLGRLDPATGQVTEWLSPSGPKS
                     EPYGISAIDDVIWYSESGATPNTVVRFDPKTAKFQSWAIPGGGNIVRNTSVTREGDFV
                     LANSLVNRVTLVKITR"
     gene            complement(3580..4311)
                     /locus_tag="VNU03_24600"
     CDS             complement(3580..4311)
                     /locus_tag="VNU03_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77727.1"
                     /translation="MASRIVVRDVDAGYGAVRVLHGVSIEVREGETVALLGTNGNGKS
                     TLIKCLMGMVTPESGEIFLETDAGRVDLAGKSTEEIVGLGIALVPEGRRLFPKLTVEE
                     NLLLGAYRRAARADIAANLAYAFEAFPVLAGRRRQLAGSMSGGEQQMLAVARALMSSP
                     KILLIDEPSVGLAPILVSRVIAKIRELKEQRHLTVLMAEQNFNQATKIADRGYIIVHG
                     KIEFEGRSTKELRENELVKKYYLGV"
     gene            complement(4301..>4518)
                     /locus_tag="VNU03_24605"
     CDS             complement(4301..>4518)
                     /locus_tag="VNU03_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77728.1"
                     /translation="GLSHAEVDDILAILFGLSERGITIIMIEHIMRAVMRFSERIVVL
                     DAGELIAEGPPDEIVRNPAVEKAYLGE"
BASE COUNT          680 a         1582 c         1532 g          724 t
ORIGIN      
        1 ggagcccagc gcgagattgc tcgcgaagac cgcgatcagt cccgcggtgc ccaccccggt
       61 gggcccgagg cgatcgatca gcaggccggc gggcacgcac ccgaccgcga gggtgacgaa
      121 gaacgcggtg gagagcaggc cggcctgcac cggaccgaag cccagctccg ccatcaggag
      181 cggaagcacc gccgcgtggt tcgtgtagtt ggtggtcgcc gcccagccca tcaggcacgc
      241 ggcggccaga cgcgtcacgg ggctacgggg cgtaggagcc gaagtacggc atcgtcacga
      301 tccgggtccg cggcgtgacc ttcctcatct ccttgaccca gagatcgagg ttggccgggt
      361 cgccgtagac ggtgcggaga tccgtcggcg tgctcgtgag cggcgcctcg aagttgtccc
      421 agtgggtggg cagcaccagc ttcgggtagc cgagggcgcg gacgaggcgc ggggtgaagt
      481 cgaccgtgtt gttggagaag aggggggcga tgagcgcgac gtcgggcttg aggccggcga
      541 tggcccgctc gatgtagttg ccggagctca tgaggaagat cgtgtacttc ccggcgatcc
      601 gcagcacata aacgagggtg tcgccctcgg ggagatcgcc gaccttggtc ggcggcgacg
      661 cgggcaccga gacgaggtgg cccggcaccg cgtacttctt ggtgccgccc atcgagtgga
      721 cgctcgggaa gacctcgatg ctgtagccgt cgaactgcat gtactccccg cccttgacct
      781 ggacgatctt gccctcgggc acgccggccg cccgcatgac gttggcgtgg gtctccgagc
      841 cgacgaccat cgcgccggtc ttcttggcga tgtagggcac gtcggccatg tggtcccagt
      901 ggccgtgccc gatcaggatc tgatcggcct tcttcacgtg ctggtcgatg agggcggtgt
      961 cggtcgggag cagccggtcg tgatccagct tgttctggag gaagcccgac tcgaagcgat
     1021 tgaaccaggg atccaccagg atggtcttgt tgccgaaggt gatctcccat ccgttggtgc
     1081 cgaaccactt gaagctcacc ccggccgcgc cgggcccccc gcccgcgggc gccggctcgg
     1141 cgcccgcggc cacgccgggc aggacgagcg atccccccag gctcgcagcg gccgccgccc
     1201 ccgccacgag gccacccaag aacccgcgcc gcgacaacgg cgaccgggcg cccatcccgt
     1261 cggtatcgat gaagcacatg gcggtgtccc cttcaggcgg tcggcagggt gagaaatgcg
     1321 cgcaccacgt gccacagctg accccggctc aggccgagcg cgaccgcgtg cgaggcgccg
     1381 gccaggacga tgaactgtcg atccgggttc ggcaggcggc ggaagaactc gaggaggtcg
     1441 tcctccgtcg cgatgccgtc gtgctcgccc cggaccatga ggaccggagc cttgacccgc
     1501 cgcgggtcga ccaccgggag attcgccgtc atgtcgaggt aggtcccggt cgggatcgtg
     1561 tcgccgaatg gaagctcggc gtcggccaga gcctcggcca cggccggatc cgacgtgccc
     1621 ggcttgtcgc gggtgaagat ggatcggatc atgtcgcgcc cccgggggcg ccggttgtgc
     1681 gtgcggtaga agtcgacctg ctcggcgcgc ttgccgagcg tgggcgagcc ctttcccgtc
     1741 caggtgaagg cctcgaggat cagccggtcg actcgctccg gccgtgccat ggcgaacccc
     1801 gccgcgcgca gcgccccgga ggagcctccg tagaagtgag cccgcggctg gccggtcatc
     1861 cggacgacga cctcgctcgc ggccttgaga tcctcaaccc cgctcgcgac gtcggaattg
     1921 ccggcggtgc gggccgactt gccgtagccc tcgtggtcca tcgtccacac gtcgaatccg
     1981 tatcccgcga acacgttcat cagggagtac tcgccccggc ccggcaccgt gaggtcgaag
     2041 ctcggcctgg ccgagaccga cgagccgtgg accaggaaga gcaccggccg ctccccggcc
     2101 tgcggcgggc cgattcgctt ccgatagagc gcgagcgaca cgtcgccctt gcgggccacg
     2161 tatccggcgc tccacagctc gtcggccctc ggccggtcgg cctgggcaag caacggcttc
     2221 ctcgccccat ccaccgtcac ggtcccgagg ccgaggcccg cgctcaagag cagcgcgcgc
     2281 cgcgatacgc cgcgtggatc gtccatccgc gatcctcctc gacccgcttc cttacctcgt
     2341 gatcttgacc agggttaccc gattgaccag gctgttggcg agcacgaagt cgccctcgcg
     2401 ggtgaccgag gtgttgcgca cgatgttgcc gccccccggg atcgcccagc tctggaactt
     2461 cgcggtcttc ggatcgaacc gcaccaccgt gttcggcgtt gctccggact cgctgtacca
     2521 gatgacgtcg tctatcgccg agataccgta gggctctgac ttgggtccgc tcggtgagag
     2581 ccactccgtc acctgaccgg tcgcggggtc gagccggccc agatagccgc gcgagaagtc
     2641 cgtgtaccag acgacgtcgt ctcgagtgat cgtgatccgc cgcggacgcg aggccgcatc
     2701 cggcagcgga tactcgcgga tctccatcgt catcggatcg acgctcccca cccggttgct
     2761 cccgaaggcc acgtagaaca ccgcgccctt cgagctcacc gccatcccgt agggccgcga
     2821 cttcggcgtc ggcggagtca ccagcgtgat ctcccccgtc tttggatcga gccgccccac
     2881 ccggttggcg ttctgggcgg tgaaccacag gatcccgctc cggtcaaaga tcagggtgtg
     2941 cgggtcctgc accgccggat ccggcatctt gtactcggtg accgcgccgg tcttcggctc
     3001 gagcttgccg atcaggccgc ccgcgttcgc ggtgtaccag atgttgcccg cctgatccgc
     3061 cacgagcccg tgggggcccc catgcggcgt cgggagcgga tactccttga accggcccgt
     3121 cttcggatcg agccgcccga gcacgttggc catctgcccc gtgtaccaga gggcgccgtc
     3181 ggcggtggcc agcggatcgt gcggccggga cccaggcgtg ggcaccggcc actccttgaa
     3241 cgagaccttc accgggcctg gaatcaccac gccgaccggc ttgggcttct cgggaaacgt
     3301 cttgaccagg tacgcggtca tcgtcgccaa ctggtcgggg gggatcggca cgccattgtt
     3361 gatcatcatg cgcatgacgg tgcgccatcc cgccggggtg tagccggccc cgacgcgcga
     3421 ggtcagcggg tggcagctgt tgcactgggc ggcaaccagt tccttgcccg gcccctcggg
     3481 cagctcctgc cccgaggccg ggagcgccgc gccgagcagc gcgccgacca gcgatgagag
     3541 cactgccaat gagagcactg atttgctagg catggctcgt cacaccccca gatagtactt
     3601 cttcaccagc tcgttttctc ggagctcctt cgtgctgcgg ccctcgaact cgatcttgcc
     3661 gtgcacgatg atgtagccgc ggtcggcgat cttggtcgcc tggttgaagt tctgctccgc
     3721 catcagcacc gtgaggtgac gctgctcctt cagctcccgg atcttggcga tcacccggct
     3781 caccaggatg ggagcgagcc cgaccgatgg ctcgtcgatc aggaggatct tcggggagga
     3841 catgagcgcg cgggccaccg cgagcatctg ctgctcgccc ccgctcatgc tgcccgcgag
     3901 ctggcgccgg cgcccggcca gcaccgggaa ggcctcgaag gcgtaggcga ggttggccgc
     3961 gatgtcggcg cgggccgcgc gacggtaggc gcccaggagc aggttctcct ccaccgtgag
     4021 cttcgggaag agccggcgcc cctccggcac cagcgcgatg ccgagaccca cgatctcttc
     4081 ggtggacttg ccggcgagat ccacgcgccc ggcgtcggtc tcgaggaaga tctcgcccga
     4141 ctcgggcgtc accatgccca tcaggcactt gatcagcgtg ctcttgccgt tgccgttggt
     4201 gccgagcagg gcgacggtct ccccttcccg cacctcgatc gacaccccgt ggagcacgcg
     4261 caccgcgccg tagccggcgt ccacgtcgcg gaccacgatc ctactcgcca aggtaggcct
     4321 tctccaccgc ggggttgcgg acgatctcgt ccggggggcc ctccgcgatc agctctcccg
     4381 cgtcgagcac cacgatgcgc tcggagaagc gcatcaccgc ccgcatgatg tgctcgatca
     4441 tgatgatcgt gatgccccgc tcgctcagcc cgaacaggat ggccaggatg tcgtccacct
     4501 ccgcgtgcga gagcccgg
//
LOCUS       DATMCV010001074         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_464068, whole genome shotgun sequence.
ACCESSION   DATMCV010001074 DATMCV010000000
VERSION     DATMCV010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464068"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VNU03_24610"
     CDS             <1..778
                     /locus_tag="VNU03_24610"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP77729.1"
                     /translation="CDAYPYTFAANPLRNLLPLWVQEGGLPAMLGRLGEPDVRRRIGR
                     EIEERGLTSFGRVPSWDAVSIGTSRTRSRDAGRSLGELARSAGVAPIDLVHDLLAADQ
                     GATFVAVSSIDEADVRELLRSPAVLVGSDGRSVAPDRVAGQGRPHPRFYGTFPRVLGH
                     YARDLGLLSLPDAVFKMTGGPARALGLRDRGLLREGYRADVTVFDPATIIDRATFETP
                     QQYPAGIATTIVNGVVVVEDGRHTGARPGRVLRRGAAGVA"
     gene            complement(788..1657)
                     /locus_tag="VNU03_24615"
     CDS             complement(788..1657)
                     /locus_tag="VNU03_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77730.1"
                     /translation="MRSEAWRRFRKHRLASVGAAILVVMVIAVVAGPFIYRVPIDEID
                     FKAKLKAPSWAHPLGTDDLGQDLLARMLYGGRISLAVGVVAMLIAITAGTMIGALAGQ
                     LGGSVDHVLMRFTDLFLSLPQLPLLLLLAYLFRDALKKALGPEMGVFMLMVVVIGGLR
                     WMPVARLVRAQFLSLREKEFVEAARGLGAAPLRQVVRHILPNAVGPVIVAATIDVAAA
                     ILAESSLSFLGLGFPPDIPTWGRILFDAKDNLDFAPHWAIFPGTAIFLTVLSINYIGD
                     GLRDALDPRRVLS"
     gene            complement(1739..>2134)
                     /locus_tag="VNU03_24620"
     CDS             complement(1739..>2134)
                     /locus_tag="VNU03_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77731.1"
                     /translation="PVAVLGLAQGASMTRFVRSAMLEVIRLDYINTARAKGLTEKVTI
                     LKHAVRNALIPVVTLVALQIPGIFTGAVITEQIFRVPGIGSLLITSILANDTPVIMAI
                     TFVFSCLVVLFNLVADLLYGWLDPRISFR"
BASE COUNT          329 a          725 c          807 g          273 t
ORIGIN      
        1 ttgcgacgcc tatccgtaca ccttcgcggc caacccgctc cgcaacctgc ttcctttgtg
       61 ggtgcaggaa ggcggcctgc cggccatgct cgggcgcctc ggcgagccgg acgtcagacg
      121 gcggatcggg cgcgagatcg aggagcgcgg gctcaccagc ttcggccgcg tcccgtcgtg
      181 ggacgcggtc agcatcggga cctcgcgcac ccggtcgcgg gacgcggggc gaagcctcgg
      241 agagctcgcg cggagcgccg gggtggcgcc gatcgacctg gtgcacgacc tcctggccgc
      301 ggaccagggc gcgaccttcg tcgcggtctc gtccatcgac gaggccgacg tgcgggagct
      361 gctccgctcg cccgcggtgc tcgtcggatc ggacgggcgg tcggtcgcgc cggaccgcgt
      421 ggccgggcag ggccggccgc acccgcgctt ctacggcacg ttcccgcggg tgctcggcca
      481 ctacgcgcgc gatctcggat tgctgtcgct cccggacgcg gtgttcaaga tgaccggcgg
      541 gccggcccgg gcgctcgggc tccgcgatcg cggcctcctg cgcgagggct accgcgccga
      601 cgtcaccgtc ttcgaccccg cgacgatcat cgaccgggcc acgttcgaga cgccgcagca
      661 gtacccggcg ggcatcgcga ccacgatcgt caacggggtg gtggtcgtcg aggacgggcg
      721 gcataccggc gcgcggccgg ggcgggtgct gcgacggggc gcggccgggg tcgcctgagc
      781 ccggcggcta cgagaggacc ctgcgcgggt cgagggcgtc ccgcagaccg tcgccgatgt
      841 agttgatgct gagcacggtg aggaagatcg cggtgcccgg gaagatcgcc cagtggggcg
      901 cgaagtcgag gttgtccttg gcgtcgaaga ggatccgccc ccaggtcggg atgtcgggcg
      961 ggaagcccag ccccaggaag gacagcgacg actcggccag gatcgcggcg gccacgtcga
     1021 tggtggcggc cacgatgacc gggcccaccg cgttgggcag gatgtggcgg accacctggc
     1081 ggagcggcgc ggcccccagc ccgcgcgcgg cctccacgaa ctccttctcg cggagcgaca
     1141 ggaactgggc gcgcaccagc cgggccaccg gcatccagcg caggccgccg atgacgacca
     1201 ccatcagcat gaacacgccc atctcgggcc ccagggcctt cttcagcgcg tcgcggaaga
     1261 ggtaggccag cagcaggagc agcgggagct ggggcaggga caggaagaga tcggtgaagc
     1321 gcatcaggac gtggtcgaca ctgccgccca gctgcccggc gagggccccg atcatcgtcc
     1381 cggcggtgat cgcgatgagc atcgcgacca cgcccaccgc gagcgagatg cgcccgccgt
     1441 agagcatccg ggcgagcaga tcctgcccga ggtcgtcggt gccgagcggg tgggcccacg
     1501 agggcgcctt cagcttggcc ttgaagtcga tctcgtcgat cgggacccgg tagatgaagg
     1561 ggccggccac caccgcgatc accatcacca ccaggatggc ggccccgacg ctcgcgaggc
     1621 gatgcttgcg gaagcgccgc cacgcctcgc tccgcatcga ttgcgccccg cggacgggcg
     1681 cgcgggccac cgtgggggag accgcctccg acgcgacgcc gttgggtggg gtcatcggct
     1741 agcggaacga gatgcgcgga tcgagccagc cgtagagcag gtccgccacc aggttgaaga
     1801 gcaccaccag gcaggagaac acgaaggtga tcgccatgat caccggggtg tcgttggcga
     1861 ggatcgaggt gatgagcagc gagccgatgc cgggcacccg gaagatctgc tcggtgatga
     1921 ccgcgccggt gaagatgccg gggatctgga gcgccaccag ggtgaccacc gggatcaggg
     1981 cgtttcgcac cgcgtgcttg aggatcgtca ccttctcggt gaggcccttg gcccgcgcgg
     2041 tgttgatgta gtcgagccgg atgacctcga gcatcgcgga gcgcacgaag cgggtcatcg
     2101 acgcgccctg ggcgaggccg agcaccgcca ccgg
//
LOCUS       DATMCV010001075         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_858681, whole genome shotgun sequence.
ACCESSION   DATMCV010001075 DATMCV010000000
VERSION     DATMCV010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858681"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..817)
                     /locus_tag="VNU03_24625"
     CDS             complement(35..817)
                     /locus_tag="VNU03_24625"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWP77732.1"
                     /translation="MDSYLMLFAFVLAEQIGLPIPAVPLLLGVGAFAGTGRMSALAGL
                     ATALAASLPPDVVWYELGRARGKRVLDVICRLSLEPDSCVRRTENLFMTRGRWALLVA
                     KFLPGLSTIAPPLAGIVGLGRVSFLVLDIMGALLWAGTWITLGYVSSSALAPLLELGA
                     RVGHWGLAAGAVALAGYVLVKFVRRRRFLRSLRVARIEPAELKRRLDAADPDLIVIDT
                     RSALDVGAAPHKIPGALWIAAETIEGRRAEIPLDRKVVLYCS"
     gene            915..1322
                     /locus_tag="VNU03_24630"
                     /pseudo
     CDS             915..1322
                     /locus_tag="VNU03_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
     gene            1473..>2635
                     /locus_tag="VNU03_24635"
     CDS             1473..>2635
                     /locus_tag="VNU03_24635"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77733.1"
                     /translation="MEEERTTFETLEAAFVEGRLSRRELLRRSALIGASAAAAHLLGS
                     LGLARPAHAQGTPKKGGTLVIAKESELDILDPHSAGGWVTWRVSKQMHEGLIDEDLTQ
                     ANVPYPKLIPKLATSWDVSKDSLTYTFKLRKGVKFHDGTPFDSAAVKYNIDRCAAKDS
                     PQFYARANAYTTWIWQFLKEVKTPDEGTVQIVLREPYGDFLRQLVQGGGGAAVFISPT
                     ALKKYGNEGIAEHPTGTGPFRFVERVRGEKVVTERNPNYWGTQPFLDRIIDRPMPEPA
                     ARMTALQAGEVDMIYVPHPDGIAELKTKGFKLEQGPAPHVWYFAFNLKDKNYQDVRVR
                     RAINMAIDREGMAKQLLKGTAVAARGLQAPCCPSYDPNFKDYPYDPAQAKKLLA"
BASE COUNT          460 a          919 c          933 g          323 t
ORIGIN      
        1 ggagcgccac ccgggcgctc gtggcctcgt tgggtcagga gcagtagagg acgaccttcc
       61 gatcgagggg aatctcggcg cggcggccct cgatggtctc ggcggcgatc cagagggcgc
      121 cggggatctt gtggggggcc gcgcccacgt cgagggccga gcgggtgtcg atcacgatca
      181 ggtcggggtc ggccgcgtcg aggcgacgct tgagctccgc cggctcgatc cgggccaccc
      241 ggaggctgcg caggaagcgc cggcggcgca cgaacttgac caggacgtag ccggcgagag
      301 ccaccgcgcc ggccgcgagg ccccagtggc ccacccgcgc ccccagctcg agcagcgggg
      361 ccagcgcgct gctcgagacg tagcccaggg tgatccacgt cccggcccag aggagggcgc
      421 ccatgatatc gagcacgagg aacgacacac gcccgaggcc gacgatgccg gccagcggcg
      481 gcgcgatggt gctgagcccg ggcaggaact tggcgacgag cagggcccag cggccgcgag
      541 tcatgaacag gttctcggtg cggcggacgc acgagtccgg ctcgagggag agccgacaga
      601 tgacgtcgag gacccgcttg ccccgcgcgc gcccgagctc gtaccagacg acgtcggggg
      661 gcagggaggc ggccagcgcg gtggcgaggc ccgcgagcgc actcatgcgg ccggtgcccg
      721 cgaaggcccc gacccccagc agcagcggga ccgcggggat cggcagaccg atctgctcgg
      781 ccagcacgaa ggcgaagagc atgaggtagc tgtccacggg tacatcctag cttgacaggg
      841 tgccggatcg cgctatacgg gagcgggagg atcgcgacat ggccggcgtg acggtgcggg
      901 gcggcatcgg gagcctggag caagaggtga ccgtgggacg gcatcgcctg gtgggcgacg
      961 agccggaggg gcagggcggt cacgacgcgg gcccgtcgcc ctacgactac ctgctggccg
     1021 cgctcggtac ctgcacgtca atgacgctca cgctgtacgc gcgacgcaag caatggccgc
     1081 tcgagaccat caccgtgaat ctccagcact cgcgcatcca cgcgacggat tgcgccgaat
     1141 gcgagacgaa ggacggtcac atcgaccgga tcgaccgcga gatcgccctc ggggggccgc
     1201 tcgacgacga gcagcgcgcg cggctgctcg agatcgccga tcgctgcccg gtgcaccgca
     1261 ccctgacctc cgagatcaat atccgcaccc gcctggccca gggggcgccg ccggcctctt
     1321 gacaaggact ccgggccggg tgtactcatc ggcccaacca acgacgctac gggttgtgtt
     1381 ctctcttcag cgcggtgtgc tcgcaacgat gcggtgccca gttcacttgc aacccgggct
     1441 cgctcagcgg gcgccgggcg gaggagggtg tcatggaaga agagcgcacc acgttcgaga
     1501 ccctcgaggc ggcattcgtc gagggacggc tcagccggcg tgagctgctt cgccgcagcg
     1561 ccctgatcgg ggccagcgcc gccgccgcgc acctcctcgg ctcgctcggg ctcgcccggc
     1621 cggcgcacgc gcaggggacg cccaagaagg gcggcaccct cgtcatcgcc aaggagagcg
     1681 agctcgacat cctcgacccg cacagcgcgg gcggctgggt gacctggcgg gtcagcaagc
     1741 agatgcacga gggcctgatc gacgaggacc tcacccaggc caacgtgccc tacccgaagc
     1801 tcatccccaa gctcgccacc tcgtgggacg tctccaagga ctcgctgacc tacaccttca
     1861 agctccgcaa gggggtcaag ttccacgacg gcaccccgtt cgactcggcg gcggtgaagt
     1921 acaacatcga ccgctgcgcg gccaaggact cgccccagtt ctacgcgcgg gccaacgcct
     1981 acaccacgtg gatctggcag ttcctcaagg aggtgaagac ccccgacgag ggcaccgtgc
     2041 agatcgtcct gcgcgagccc tacggggact tcctccgcca gctcgtccag ggcggcggcg
     2101 gcgccgcggt cttcatcagc cccaccgcgc tcaagaagta cggcaacgag ggcatcgccg
     2161 agcacccgac cgggaccggc ccgttccgct tcgtggagcg cgtgcgcggc gagaaggtgg
     2221 tgaccgagcg gaaccccaac tactggggga cgcagccgtt cctcgaccgg atcatcgacc
     2281 ggccgatgcc ggagccggcc gcgcgcatga ccgcgctgca ggccggcgag gtcgacatga
     2341 tctacgtgcc gcacccggac ggcatcgcgg agctgaagac caagggcttc aagctcgagc
     2401 aggggccggc cccccacgtc tggtacttcg cgttcaacct gaaggacaag aactaccagg
     2461 acgtgcgcgt gcggcgggcc atcaacatgg ccatcgaccg cgagggcatg gccaagcagc
     2521 tcctgaaggg gaccgcggtc gccgcgcgag gtctgcaggc gccgtgctgc ccgtcctacg
     2581 atccgaactt caaggactat ccctacgacc ccgcgcaggc caagaagctc ctcgc
//
LOCUS       DATMCV010001076         6337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_792233, whole genome shotgun sequence.
ACCESSION   DATMCV010001076 DATMCV010000000
VERSION     DATMCV010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792233"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VNU03_24640"
     CDS             complement(<1..641)
                     /locus_tag="VNU03_24640"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77734.1"
                     /translation="MQKALAMWILLAAGGGGQPEVSPTQVVQQATEHVLQVVQDAELA
                     APAIQERRRVEVQRIADRLFDFPEMARRALAIHWRDRTPQEQGEFVFVFKQLLARAYL
                     GKLENYSGEQIVYLGETVDGDFATVRSKIVTGRGAEIPVDYRLHLVGSRWAVYDVAVQ
                     GVSFVANYRGQFDKIIRSSSYSSLMRDLRAKYAQATGRATVGATPIPASPAPTP"
     gene            complement(835..1236)
                     /locus_tag="VNU03_24645"
     CDS             complement(835..1236)
                     /locus_tag="VNU03_24645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77735.1"
                     /translation="MLSYLAMAPTGERPRVLVVDDESIIAQLIADVLGGEGFDVDTAP
                     HGLAALDRLANRTYDIVLSDLRMPELDGLGLFREIEQRYPDLLRRFVFITGTSEHTDY
                     QGFIDDVKVPVLTKPFDMMNLVRVVQTRLAG"
     gene            complement(1346..2425)
                     /locus_tag="VNU03_24650"
     CDS             complement(1346..2425)
                     /locus_tag="VNU03_24650"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HWP77736.1"
                     /translation="MAAPETIERGPTERLQFLDAVRGFALLLMIVNHTSRWWQDGTMG
                     WPRYNLIYASMVVGAPLFLFLVGFCLPLSFARRRPTGGLAGVAAKFLQRGGRLILASW
                     LLNFVVFRDEPFWEGGVLQTIGLGIILALPPMLLLHRRGARTLLIAIALGAYALFSVS
                     FAQVTAWLATHPTLGRIFLFEFAPWPWISLVWIGLALGWTWALQDTPARRARYLTIMS
                     AVGVVFLALFVAWDWAHGTPPHLSLAFKRDFILNNHWISRGVTNFLCLGSVFCILGLA
                     YYLIEVRRLPVGWLVVLGRTSLMLYFLHHVLVFTIAHEWLGLVLDRWWWFVLANALLT
                     VVLVYLGRLWLEIRSLAARRRLQTA"
     gene            complement(2445..4082)
                     /locus_tag="VNU03_24655"
     CDS             complement(2445..4082)
                     /locus_tag="VNU03_24655"
                     /inference="COORDINATES: protein motif:HMM:NF006122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWP77737.1"
                     /translation="MAATPMTGGEALVRSLYQEGVRVVFGLPGVQLYGVMAALRDEPR
                     IRFVNTRHEQATSFMADGYARAGGSFGTALVVPGPGLLNAMSGLSTAYSASSPVLMLS
                     GQIPRDSIGRDLGLLHEVNDQQECIRPVTKWRRRVLQVADVPAAVREAVVQLKSGRPR
                     PVEIEMPPETMEDEGLVELLPPAEVTRHAAAARDLDRAAELLLASRAPLIYAGGGVHL
                     AGAHDALAQVAEHLQAGVAQSAEGKGAVSDHNTLSLGAAFWRDSALRAHIHEADVVLA
                     VGSRLAGVSFKPGTRIIQIDADEQEIGRSHKDTLGLVGDARATLEALLERLRAASAPR
                     PSRKAEHEALRAKMAGENTMEPNASIIKSLRAGAPENTILVAGMTQIGYYSRPFWPVY
                     EPRTYLSSSYSGNLGYAYPVALGAKVARPDRPVVSVSGDGGFLFNAQELSTAARHGIN
                     VVAVVFNDSSYGNVARDLDESWGGQYGAELANPDFMKLADAYGVVGMRAKEPTDVGRL
                     VREAIEKDRPALIEVPVGRMSRPAFFTPRRTPLKHPR"
     gene            complement(4105..5313)
                     /locus_tag="VNU03_24660"
     CDS             complement(4105..5313)
                     /locus_tag="VNU03_24660"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWP77738.1"
                     /translation="MRDAAGPLSDLVILDLTRVLAGPYGTRLLADLGARVIKIERPRD
                     GDDMRKNHLQVEPGRTDQSTYFIRINAGKESVAVDLSHPQGRAVVIDICRVADVFVEN
                     FAPGVVEKLGLGYAAMAAVKPDLVYCSISGFGQTGPWRERQAFAHIINAASGMMHLDQ
                     GDQEAPRASNLQAADVLAGTHAFGAILAALRRRDRTGQGAYLDVSMLEALVAADDVSY
                     AAVLNLGETQGSPRPAMGVYAVGGRHLALQTGGAAALWPRLQELLGRPELATDPRFTT
                     PDQRRRNWPALRDIIGEWVGRFASVEEALDALRAARIPAAAVLRPDEVAVHPHLAARG
                     AFPTVTHPTRGAVRVTAAPFQLDGQPVAPAGPAPYRVGEHTRAVLGELLGYKDRDIDD
                     LVQAGVIEAP"
     gene            5370..6164
                     /locus_tag="VNU03_24665"
     CDS             5370..6164
                     /locus_tag="VNU03_24665"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWP77739.1"
                     /translation="MRRNQLRERLRAGRPTRGTHIHSAWPAVVELAGHAGCFDYVEFV
                     GEYAPYDLFALENLARAVNTFDHMSAMFKVEQQPRTYLAVRAIGAGIQNLLFADPRTP
                     DDVRECVRSVRAETPEAGGLHGVGMRRDVGYVVDVGSPAFVQALDDAVVAIMIEKASA
                     VENLEALLSVKGVDMVQFGPADYSMSIGLAGQWNHPRVVEAERHVIATALRLGIAPRA
                     EISHPNEAKAYLDLGVRHFCMGWDVSILFDWFKAEGGALRDLLGAG"
BASE COUNT          935 a         2216 c         2329 g          857 t
ORIGIN      
        1 ggggtggggg cgggggaggc cgggatggga gtggcgccca ccgtcgcgcg cccggtcgcc
       61 tgcgcgtact tcgcccgcag atcgcgcatc agggacgagt acgacgagct ccggatgatc
      121 ttgtcgaact ggccccggta gttcgccacg aagctcacgc cctggaccgc gacgtcgtac
      181 accgcccagc gtgagccgac caggtgcagg cgatagtcca ccgggatctc cgcgcctcgg
      241 ccggtgacga tcttcgagcg cacggtggcg aagtcgccgt ccaccgtctc gcccaggtag
      301 acgatctgct cgccggagta gttctcgagc ttgcccagat aggcgcgggc gagcagctgc
      361 ttgaacacga acacgaactc gccctgctcc tgcggggtgc ggtcccgcca gtggatcgcg
      421 agggcccggc gggccatctc ggggaagtcg aagaggcgat cggcgatgcg ctggacctcg
      481 acccggcgcc gctcctggat cgcgggggcc gccagctcgg cgtcctggac gacctgcaag
      541 acgtgttcgg tggcctgctg gacgacctgg gtcggactca cttccggctg ccctccgcca
      601 cccgcggcga gcaggatcca catggccagt gccttctgca tgacaacctc cgggggccgc
      661 gtctagctgt gggctcgcgg ccacttcgtc ccctgcgacc tccaagagca tgccccgtgc
      721 cacgagggag ccgggctaac cggtgggatt acctggcttc gggaggccgg gggagcctcg
      781 gacatcgtca gtgccgggac gccgtcaggc ggccgacggc agtggtgggt cgagctagcc
      841 ggccaggcga gtctgcacga cgcggacgag attcatcatg tcgaacggct tggtgaggac
      901 ggggaccttc acgtcgtcga tgaagccctg atagtccgtg tgctcggacg taccggtgat
      961 gaagacgaag cggcgcagca ggtcgggata ccgctgctcg atctcgcgga acaggccgag
     1021 cccgtcgagc tcgggcatgc gcagatcgct caggacgatg tcgtaggtgc gattcgcgag
     1081 acggtcgagc gccgccaggc catgcggcgc ggtatcgacg tcgaagccct cgccgcccag
     1141 cacgtcggcg atgagctgcg cgatgatgct ctcgtcgtcg acgaccagca cgcgaggacg
     1201 ctcgccggtg ggcgccatgg ccagatagga cagcaagagg cgcgccaggg ggaattgacc
     1261 gccaaatcag tcgattggcg ccgtcttcgc ggcgcgcggg cggaattcgt cagtgggccg
     1321 gcccggaaat gagcaaccgt ccggctcagg cggtctgcag gcggcggcgc gcggccaggc
     1381 tccggatctc gagccacagc cgtcccaggt agacgagcac gacggtgagc agcgcgttcg
     1441 cgagcacgaa ccaccaccag cggtcgagca cgaggccgag ccactcgtgc gcgatcgtga
     1501 agaccagcac gtggtggagg aagtagagca tcagcgacgt gcggcccagc accaccagcc
     1561 agcccaccgg caggcgccgg acctcgatca ggtagtacgc gagcccgaga atgcagaaca
     1621 ccgacccgag gcagaggaag ttcgtgaccc cgcgcgagat ccagtgattg ttcaggatga
     1681 agtcgcgctt gaaagcgagc gacaggtgcg gcggcgtgcc gtgggcccag tcccaggcca
     1741 cgaagagggc gaggaagacc accccgaccg cgctcatgat cgtgaggtat cgcgcccggc
     1801 gcgccggggt gtcctgcagc gcccacgtcc accccagcgc caggccgatc cacacgagcg
     1861 agatccaggg ccacggcgcg aactcgaaca ggaagatgcg acccagggtc gggtgggtgg
     1921 ccagccaggc ggtgacctgc gcgaaagaaa ccgagaagag cgcgtaggcc ccgagcgcga
     1981 tcgcgatgag cagggtgcgg gcgccgcgcc ggtgcagcag gagcatcggc ggcagcgcga
     2041 ggatgatccc gagcccgatc gtctggagca cgccgccttc ccagaacggc tcgtcgcgga
     2101 agactacgaa gttgagcagc caggaggcga ggatcaggcg cccgccgcgc tgcaggaact
     2161 tggccgccac cccggccagg ccgccggtgg ggcggcggcg cgcgaaggag agcggcaggc
     2221 agaagccgac caggaacagg aagagcggcg ccccgaccac catgctcgcg tagatcaggt
     2281 tgtagcgggg ccagcccatg gtgccgtcct gccaccaccg gctggtgtgg ttgacgatca
     2341 tcaggagcag cgcgaatccc cgcaccgcgt ccaggaactg cagccgctcc gtcgggccgc
     2401 gctcgatcgt ttccggcgcc gccatccagt cctccgtcgc cgcgctagcg cggatgcttg
     2461 agcggagtcc gccgcggggt gaagaacgcg ggccggctca tccgccccac cggtacctcg
     2521 atcagcgccg ggcggtcctt ctcgatcgcc tcgcgcacca gccggccgac gtcggtcggc
     2581 tccttcgccc gcatgccgac caccccgtag gcgtcggcca gcttcatgaa gtccgggttg
     2641 gcgagctcgg cgccgtactg cccgccccag gactcgtcca ggtcgcgcgc cacgttgccg
     2701 taggagctgt cgttgaagac caccgcgacc acgttgatcc cgtgccgcgc ggcggtggag
     2761 agctcctggg cgttgaagag gaagccgccg tcgccggaga cggagaccac cggccggtcc
     2821 ggccgggcga ccttggcccc cagcgcgacg ggatacgcgt acccgaggtt gccggagtac
     2881 gacgacgaga gataggtgcg cggctcgtag accggccaga agggccgcga gtagtagccg
     2941 atctgggtca tgcccgcgac caggatcgtg ttctcgggcg ctcccgcgcg cagcgacttg
     3001 atgatcgacg cgttgggctc catcgtgttc tcgcccgcca tcttcgcgcg cagcgcctcg
     3061 tgctcggcct tgcgcgaggg ccgcggggcg gacgccgcgc gcaggcgctc caggagggcc
     3121 tccagcgtgg cccgcgcgtc gccgaccagg ccgagcgtgt ccttgtggct gcgcccgatc
     3181 tcctgctcgt ccgcgtcgat ctggatgatg cgggtgccgg gcttgaagga gaccccggcc
     3241 aggcggctcc ccaccgcgag caccacgtcc gcctcgtgga tgtgggcgcg cagcgcggag
     3301 tcgcgccaga acgcggcccc gagcgagagc gtgttgtggt cgctgaccgc gcccttgcct
     3361 tcggcggact gggcgacccc ggcctgcaga tgctcggcga cctgggcgag cgcgtcgtgc
     3421 gcgcccgcga ggtgcacgcc gccgcccgcg tagatcagcg gggcgcgcga ggccagcagc
     3481 agctcggcgg cgcggtcgag gtcgcgcgcg gccgcggcgt ggcgcgtcac ctcggcgggc
     3541 ggcagcagct cgaccagccc ctcgtcctcc atggtctcgg gcggcatctc gatctcgacc
     3601 gggcggggcc ggccgctctt gagctgcacc accgcctcgc gcaccgcggc cggcacgtcc
     3661 gccacctgga gcacgcgccg gcgccacttg gtgaccgggc ggatgcactc ctgctggtcg
     3721 ttgacctcgt gcaggagccc gaggtcgcgc ccgatgctgt cgcgcgggat ctggccggag
     3781 agcatcagca ccggcgacga cgcggagtac gcggtgctga ggccggacat ggcgttgagc
     3841 agcccggggc ccggcaccac gagggcggtg ccgaagctac cgccggcgcg cgcgtacccg
     3901 tcggccatga agctggtcgc ctgctcgtgg cgcgtgttga cgaagcggat ccgcggctcg
     3961 tcacgcaggg cggccatgac cccgtagagc tgcacgccgg ggaggccgaa gaccacccgc
     4021 acgccttcct ggtagaggga ccgcaccagt gcttcgccgc ccgtcatcgg tgtcgccgcc
     4081 atgtgtcgtc tccttcgacc cgtctcaggg cgcctcgatg acgccggcct ggacgaggtc
     4141 gtcgatgtcg cgatccttgt agccgagcag ttcgcccagc accgcgcggg tgtgctcgcc
     4201 cacccggtag ggcgcggggc ccgcgggcgc caccggctgc ccgtcgagct gaaacggcgc
     4261 cgcggtcacc cgcaccgcgc cgcgggtcgg atgggtgacc gtcgggaacg ccccgcgcgc
     4321 ggccaggtgc gggtggacgg ccacctcgtc cggcctgagc accgcggcgg ccgggatgcg
     4381 cgcggcccgc agcgcgtcca gcgcctcttc cacggatgcg aagcgaccga cccactcgcc
     4441 gatgatgtcg cggagggccg gccagttgcg ccggcgctga tccggggtgg tgaagcgcgg
     4501 gtcagtggcg agctccggcc gcccgagcag ctcctggagc cgcggccaca gggcggccgc
     4561 cccgccggtc tggagcgcca agtgccggcc gccgaccgcg tacacgccca tggccggacg
     4621 cggcgagccc tgcgtctcgc ccaagttgag caccgccgcg taggagacgt cgtcggccgc
     4681 gacgagcgcc tcgagcatcg acacgtcgag gtaggcgccc tgaccggtgc ggtcccgccg
     4741 gcgaagcgcg gcgaggatgg ccccgaaggc gtgggtgccc gccagcacgt cggcggcctg
     4801 gaggttcgac gcgcgcggcg cctcctggtc gccctggtcc aggtgcatca tgccggaggc
     4861 cgcgttgatg atgtgggcga aggcctgccg ctcgcgccac ggcccggtct gtccgaagcc
     4921 cgagatcgag cagtagacga ggtcgggctt gaccgccgcc atcgcggcgt agcccaggcc
     4981 gagcttctcc acgaccccgg gagcgaagtt ctcgacgaag acgtcggcca cccggcagat
     5041 gtcgatgacc accgcgcggc cctgcgggtg gctcaggtcg accgccacgc tctccttgcc
     5101 cgcgttgatg cggatgaagt aggtggactg gtcggtgcgg cccggctcga cctggaggtg
     5161 gttcttgcgc atgtcgtcgc cgtcgcgcgg ccgctcgatc ttgatgaccc gcgcgcccag
     5221 gtccgcgagc aggcgggtgc cgtagggacc ggccaggacg cgggtgagat cgaggatcac
     5281 gaggtcggag aggggcccgg cggcgtcgcg caccggccga gtatagcgcg gcgattgacg
     5341 gggcgcacct gcccccctag actcggcgca tgcgacgcaa tcagctgcgg gagcgcctgc
     5401 gggcgggccg gcccacgcgc ggcacgcaca tccactccgc gtggccggcg gtggtggagc
     5461 tggccggcca cgcgggctgc ttcgactacg tggagttcgt gggcgagtac gcgccctacg
     5521 acctcttcgc gctggagaac ctcgcgcggg cggtgaacac cttcgaccac atgtcggcga
     5581 tgttcaaggt cgagcagcag ccgcgcacct atctcgcggt gcgggccatc ggggcgggga
     5641 tccagaacct gctcttcgcc gatccccgca cccccgacga cgtgcgcgag tgcgtgcgca
     5701 gcgtgcgcgc ggagaccccg gaggcgggcg gcctgcacgg ggtcggcatg cggcgcgacg
     5761 tgggctacgt ggtcgacgtc ggctcgcccg cgttcgtgca ggcgctcgac gacgcggtgg
     5821 tcgcgatcat gatcgagaag gcctccgcgg tcgagaacct ggaggcgctg ctgtcggtga
     5881 agggcgtcga catggtgcag ttcggccccg ccgactactc gatgagcatc ggtctcgcgg
     5941 ggcagtggaa tcacccccga gtggtcgagg cggagcgcca cgtgatcgcc accgccctgc
     6001 gcctcggcat cgccccgcgc gcggagatct cgcatccgaa cgaggcgaag gcctacctcg
     6061 atctgggggt gcggcacttc tgcatgggct gggacgtgtc gatcctcttc gactggttca
     6121 aggccgaggg cggcgccctg cgcgacctgc tgggcgccgg atagccggac cgcgaccctc
     6181 gcccatggcg ccccgcccgg cgcgcgccgc ggccgctctt gtcgtgctgc tcgccctgct
     6241 gaccgcggcc gcgtgccgcg agcgcccgcc ggccggcggc ccggtcacgc tcgtcttcaa
     6301 gcacgccaag atcctcggcc ccgccgatcc ggtgccc
//
LOCUS       DATMCV010001077         2946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_259446, whole genome shotgun sequence.
ACCESSION   DATMCV010001077 DATMCV010000000
VERSION     DATMCV010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259446"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..995)
                     /locus_tag="VNU03_24670"
     CDS             complement(51..995)
                     /locus_tag="VNU03_24670"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWP77740.1"
                     /translation="MGNAPSKTVDPEQDQRRCPRQRVSWLVSVAAGQRSFQGLTRDVS
                     ASGAKILVKERPALGAEISLSFRPPGRRPVETRALVWRVDPDGLACMFVGTQEAEFLA
                     AVAPRTVSAPRTPPPAPVAAPVSATLVSAVAAPVAPPPLPAVAPAPVAPPPRPPVAPA
                     APPPRPAVAPAPVAPPPRAAVAPAPVAPPPAPSPTRSGGSVLIAASDTGLRALAIAGL
                     ERSGYAVLDAGSQPLLALRMAEQHGGAIVVALIAADLKLMNGEPLAGRLAPLLPVAKL
                     VLMASRTAPSPSTPGALWLPTPCSDAEMMAAVRQALAG"
     gene            complement(1149..2015)
                     /locus_tag="VNU03_24675"
     CDS             complement(1149..2015)
                     /locus_tag="VNU03_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWP77741.1"
                     /translation="MRSKLFVPASRPELFAKALAGDADAISFDLEDAVQESRKAEARG
                     VLKTFLRQSSPRPGGKIVIVRVNGLTTPHFEADLAAVAGPVVDMINLPKPESADDVRA
                     AAAALAGCERANGVERPIGILANIESPRGLRRAAEIAGAHPRVVGLQLGLGDLFEPFG
                     IDRVDALAVHAMQLAVRLAAAEAGLWACDTVYGGVGDPDGYTREATAARRLGYLGKSC
                     IHPSQVPLANAVFRPTDGEIAAARRVMEAARGAEARGVGAFLVDGRMIDIPFIKRAEA
                     ILGAARRLDLLS"
     gene            complement(2033..>2946)
                     /locus_tag="VNU03_24680"
     CDS             complement(2033..>2946)
                     /locus_tag="VNU03_24680"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017133176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP77742.1"
                     /translation="FRPSVPARLGIDYDRMKAINPRLIYCALTGYGDTGPLKDRAGYD
                     QVLQTMSGICVEQGKPGDPEIVYGSAVDFYAASMLAFAVSAALFERSHVGVGRYVGVS
                     LLRSALTMQATRLVWVDGEGRDVARDMRSGGITGLHPTRSGSLYISANTPHFWKALCE
                     LTGLAELADDPRFDTVRKRAEHAALLVPRLREALQAHTALEWEARFGERVPCAAARPV
                     EDMFDHPQVAAEGILAHFEHGRVGRYRGMAHPVHFGGGPAATPFTAPELGQHSRKILA
                     GLGYSTEEIERLCRGGAVVAPEKDTGA"
BASE COUNT          415 a         1072 c         1112 g          347 t
ORIGIN      
        1 ggggacgcga cgaccgcgcg tgcggcggcg cggtccgggg acgggtgcgg tcagccggcg
       61 agggcctgcc ggacggccgc catcatctcg gcatccgagc agggagtggg cagccagagc
      121 gcgccggggg tcgatgggct gggcgcggtc cggctcgcca tgagcacgag cttggcgacg
      181 ggcagaagcg gcgcgagacg gccggccagc ggctcgccgt tcatcagctt gagatccgcc
      241 gcgatcaggg cgacaacgat cgcaccgcca tgttgctccg ccatccggag ggccaggagc
      301 ggctgcgacc ccgcgtcgag caccgcgtat ccactgcgct cgagtccggc gatggccagc
      361 gcccggagcc cggtgtcgga cgcggcgatg agcacgctcc ctcccgacct ggtcgggctc
      421 ggggcgggcg gcggcgccac cggggccggg gccacggccg cgcggggcgg cggagcgacc
      481 ggagcgggag ccaccgccgg gcggggcggc ggagcggccg gggccacggg cgggcgaggc
      541 ggcggagcga ccggagcggg ggccaccgcc ggaagaggtg gcggagcgac cggggccgcg
      601 acggcggaga cgagagtagc ggagaccgga gcggccaccg gggccggcgg cggcgtccgc
      661 ggggccgaga cggtgcgggg cgcgaccgcg gcgaggaact ccgcctcctg ggtgcccacg
      721 aacatgcacg cgagcccatc ggggtcgacc cgccagacga gggcccgagt ctcgaccgga
      781 cgacgcccgg gcgggcgaaa ggagagactg atctcggcgc ccagcgcggg ccgctccttg
      841 accaggatct tggcgccgga agcgctcacg tcgcgagtga gaccctggaa gctccgctgg
      901 ccggccgcaa cgctcacgag ccacgacacc cgctggcgcg ggcaccgtcg ctggtcctgc
      961 tccgggtcga ccgtcttgct cggcgcgttt cccattggtg gcgggtgaag gttccgccgg
     1021 acgaatcgca agaatcgagc cacgccggac cgcccgtgga tcgccccggg atcgcccagg
     1081 gatcgaacga tttcgagggc gcgccggcgc ggcggcgtca ggagggtgtc acggagactg
     1141 tccgcgcgtc aggacagcag gtcgagccga cgggccgcgc cgagaatcgc ttcggcccgc
     1201 ttgatgaacg ggatatcgat catccggccg tccaccagga acgcacccac gccgcgggcc
     1261 tcggcccccc gcgcggcctc catcacgcgg cgcgccgccg caatctcgcc gtcggtcggc
     1321 cggaacaccg cgttcgcgag cgggacctgg ctcgggtgga tgcacgactt cccgaggtac
     1381 ccgagtcgac gcgccgcggt ggcctcgcgc gtgtagccgt cgggatcgcc caccccgccg
     1441 tacaccgtgt cgcacgccca gaggcccgcc tccgccgcgg ccagccgcac cgcgagctgc
     1501 atggcgtgca ccgccagggc atccacccgg tcgatcccga atggctcgaa caggtcgccg
     1561 agcccgagct gcaggccaac gacgcgcggg tgggcccccg cgatctcggc cgcgcgcctc
     1621 agcccccgcg gcgactcgat gttggccagg atcccgatgg gccgctcgac gccgttggcg
     1681 cgctcgcacc cggccaacgc ggccgccgcg gctcggacgt cgtccgccga ttcgggcttg
     1741 ggcaggttga tcatgtccac caccggcccg gccaccgcgg ccaggtcggc ctcgaagtgc
     1801 ggcgtcgtga gcccgttcac ccggacgatc acgatcttgc cgccgggccg aggagacgat
     1861 tgccggagga aggttttcaa gacgccgcgg gcctcggcct tccggctctc ctggaccgcg
     1921 tcctccaggt cgaaggagat cgcatcggcg tcgccggcga gggccttcgc gaacagctcc
     1981 ggccgggagg cgggcacgaa cagcttgctg cgcatgagga aggcggtcgc ggtcatgccc
     2041 ccgtgtcctt ctcgggggcg acgaccgcgc cgccacggca gagccgctcg atctcctcgg
     2101 tggaatagcc gaggccggcc aggatcttgc gcgagtgctg accgagctcc ggcgcggtga
     2161 acggggtggc cgccggacca ccaccgaagt gcaccgggtg cgccatcccg cggtagcggc
     2221 ccacccggcc gtgctcgaag tgggccagga tgccctcggc ggccacctgc ggatggtcga
     2281 acatgtcctc gaccgggcgg gccgccgcgc aggggacgcg ctccccgaag cgcgcctccc
     2341 actcgagcgc ggtgtgcgcc tgcagggcct cgcgcaggcg gggcaccagc agggccgcgt
     2401 gctcggcccg cttccgcacc gtgtcgaagc gcgggtcgtc cgcgagctcc gcgaggccgg
     2461 tcagctcgca cagcgccttc cagaagtgcg gggtgttggc cgagatgtag aggctgccgc
     2521 tccgggtggg gtgcagtccg gtgatcccgc cggatcgcat gtcgcgggcc acgtcgcgcc
     2581 cctcgccgtc cacccacacc aggcgcgtcg cctgcatcgt cagcgcgctg cgcagcagcg
     2641 acacgcccac gtaccggccc accccgacgt ggctccgctc gaagagcgcc gccgagaccg
     2701 cgaaggccag catggaggca gcgtagaagt cgaccgcgga gccgtacacg atctcgggat
     2761 cgcccggctt cccctgctcc acgcagatgc cgctcatggt ctgcaagacc tgatcgtacc
     2821 cggcgcggtc cttgagcggc ccggtgtccc cgtagccggt cagcgcgcag taaatgaggc
     2881 gcggattgat cgccttcatc cggtcgtagt cgatgccgag ccgcgcgggg accgacggcc
     2941 ggaagt
//
LOCUS       DATMCV010001078         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_127354, whole genome shotgun sequence.
ACCESSION   DATMCV010001078 DATMCV010000000
VERSION     DATMCV010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127354"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1313
                     /locus_tag="VNU03_24685"
     CDS             63..1313
                     /locus_tag="VNU03_24685"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HWP77743.1"
                     /translation="MSQTWIVTVGAARLGSADDPAGLLVARALLADGVPVASRQIVDE
                     DETAVEEALRSAVEAASLVVVLAHPGGSSGEVVRRALSRLTGARLVLNDKLLAQLEED
                     FARRGQAMPRRLDRLALLPQGAVLWPGAGEPAWVLESKRAAVAVLPAGSAALASLVEQ
                     QLRPLARQRAGTGESTVLRTLHTTGLAPADAEERLGRWLGADGPVAVACVPVDGDVWV
                     QLRARAQSRAAAEAALAPVEREIREALGPDCWGRDEDTLEVVVGRLLLDRHLTVSMAE
                     SCTGGLVGHRLTNIAGSSRYVERGVIVYSNQAKEQLLGVPEELLRAHGAVSAPVAEAM
                     AAGICRISGSACGIAVTGIAGPDGGTLAKPVGTVFIAAAAPGPEGPRIAVRRFRFPGG
                     REAVKWQSSQAALDMLRRALLPRA"
     gene            1310..1912
                     /gene="thpR"
                     /locus_tag="VNU03_24690"
     CDS             1310..1912
                     /gene="thpR"
                     /locus_tag="VNU03_24690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWP77744.1"
                     /translation="MIRAFVAILLGDAVREAVAAMVERLRPLGPPVAWVPAANLHLTL
                     QFLGDQPEERLAAAEAALRDAAATSAPIEVALHGIGAFPGLERPRILWVGLAQGALEV
                     RALQARVAEALSREGFPREDRAWHPHLTIGRVYDERRWRREAGPALRQALAQVATTRF
                     GTLRIAELALMRSELSPRGARYTIRHAVPLGGGEASSGPS"
BASE COUNT          210 a          749 c          811 g          266 t
ORIGIN      
        1 gctctacgcc ctggtcctcg tcatgggagc ccgggcgctc ttcggcgtgc ccgcgtgagg
       61 ccatgagcca gacctggatc gtcacggtgg gcgcggcccg gctcggctcg gccgacgacc
      121 cggccgggct cctggtcgcg cgcgctcttc tcgccgacgg cgtgccggtg gcctcgcgcc
      181 agatcgtgga cgaggacgag accgcggtcg aggaggcgct gcgctccgcc gtcgaggccg
      241 cgtccctggt ggtggtgctc gcgcaccccg gggggtcgag cggcgaggtg gtgcggcgcg
      301 cgctgtcgcg gctcaccggg gcccgcctcg tgctcaacga caagctcctg gcgcagctcg
      361 aggaggactt cgcccgccgc ggccaggcga tgccccgccg gctcgatcgc ctggccctgc
      421 tgccgcaggg cgcggtgctg tggccgggcg cgggcgagcc cgcctgggtg ctcgagagca
      481 agcgcgcggc ggtggcggtg ctgccggcgg gctcggccgc gctcgcgagc ctggtggagc
      541 agcagctgcg cccgctcgcc cgccagcgcg cgggcacggg cgagagcacg gtgctccgca
      601 cgctgcacac caccgggctc gcgcccgccg acgccgagga gcggctcggc cgctggctcg
      661 gggcggacgg gccggtggcg gtcgcctgcg tgccggtgga cggcgacgtg tgggtgcagc
      721 tgcgcgcgcg cgcccagtcc cgcgcggccg ccgaggcggc actggcgccg gtggagcggg
      781 agatccgcga ggcgctcggg cccgattgct ggggccgcga cgaggacacg ctcgaggtcg
      841 tcgtcggtcg cctgctgctg gatcggcacc tcacggtgtc gatggccgag tcctgcaccg
      901 ggggcctggt cggccaccgg ctcaccaaca tcgccggctc ctcccgctac gtcgagcgcg
      961 gcgtgatcgt ctactcgaac caggccaagg agcagctcct gggcgtgccc gaggagctgc
     1021 tgcgcgccca cggcgcggtg agcgcgccgg tggccgaggc gatggcggcc gggatctgcc
     1081 ggatctcggg ctcggcctgc gggatcgcgg tcaccggcat cgcgggcccc gacggcggca
     1141 cgctcgccaa gcccgtcggc accgtgttca tcgcggcggc cgcgccgggc cccgaggggc
     1201 ctcgcatcgc ggtccggcgt ttccgctttc cgggtggccg ggaggcggtg aagtggcagt
     1261 cctcgcaggc cgcgctcgac atgcttcgcc gcgccctgct gccgcgggcg tgatccgcgc
     1321 cttcgtcgcg attctcctcg gcgacgcggt gcgggaggcg gtggccgcga tggtcgagcg
     1381 gctgcgtccg ctgggccctc cggtggcctg ggtgcccgcc gcgaacctgc acctgaccct
     1441 gcagttcctg ggtgaccagc ccgaggagcg gctcgccgcg gcggaggccg cgctgcgcga
     1501 cgcggcggcg acgagcgcgc cgatcgaagt ggcgctccac gggatcggcg cgtttcccgg
     1561 gctcgagcgc ccgcgcatcc tctgggtcgg gctggcccag ggcgccctcg aggtccgcgc
     1621 cctgcaggcg cgggtggccg aggccctgtc ccgcgaagga ttcccgcgag aagatcgcgc
     1681 gtggcatccc catctcacga tcggacgggt gtacgacgag cgccgctggc gccgcgaggc
     1741 cggcccggcc ctgcgccagg ccttggccca ggtggcgacc acccgcttcg gcacgctgcg
     1801 aatcgccgag ctggccctga tgcgcagcga gctgtcgccg cggggagcgc gctacacgat
     1861 ccggcacgcg gttccgctgg gcggcgggga ggcgtcgagc ggcccctcct gagcgggcgg
     1921 cccacgaaca gcggagcgtc ttcgagcacg ccgccaagtg ggctccgcgc gccttccgat
     1981 ggagcgcgcg tgaccgggaa cgcgactgcg tcgtgtccgc gaacgtcacg tcgagg
//
LOCUS       DATMCV010001079         7945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_792608, whole genome shotgun sequence.
ACCESSION   DATMCV010001079 DATMCV010000000
VERSION     DATMCV010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792608"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="VNU03_24695"
     CDS             complement(<1..2079)
                     /locus_tag="VNU03_24695"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWP77745.1"
                     /translation="MTTNLIDIQNSDVIMATSNFAENHPVGFQWVMKAKERGAKFIHV
                     DPRFTRTSAVADVHVPLRSGTNIAFFGGLISYAIQNNLYFKDYVVPYTNASFLIDPAF
                     KTATDGGGLFTGLESTGKGGVDPHLAETYKRDSWKYQLDGEGNPKRDLTLRDPSSVFQ
                     LMKRHYSRYTPEMVERVCGIPKEKFVQVAKLYCENSGPEKTGSITYALNLTQHTNGVQ
                     NIRALCMLQILLGNIGRPGGGVVALRGHANVQGATDLELLYHELPGYLPMPLRDAHPD
                     LKTYLEKVTPKGGFWVNLPKFMVSLMKAFYGDAAKVENDYGYQWLPKRASADAYSHQH
                     MFVDMYKGVIKGFLADGQNPAVGGPNAKLARAALGKLDWLVVKDIFVTETAEFWKAPG
                     AVAKDIKTEVFFLPAAPAAEKDGSLTNTMRLIQWHERAVKPPGDVTSDAEFFVSLAKR
                     LQKMYAGSKKERDQGFLAASFQYGDDPKEPNMELVLKEINGYATQEITDKDGKVVYKP
                     GQALNSFAHMTDDGKTAGGCWIYTGVTVEGPDGKLINKANLRKPADDKDYLAHGWGFA
                     WPANRRILYNRASADLQGKPWSEKKKLIWWDPAAPGQEPDKKGKWVGLDVPDFNAFLA
                     PDAKNGDKPFIMRADLVGAFFGPLNDGPFPEHYEPIESPTKNLLSKQQNNPVAKIWSV
                     PDKRNDLAPMG"
     gene            complement(2128..2706)
                     /locus_tag="VNU03_24700"
     CDS             complement(2128..2706)
                     /locus_tag="VNU03_24700"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWP77746.1"
                     /translation="MSLSRRDFLKYAGSTGAGVAVGTATLDLKPVQAAATTLKIKEAK
                     VHPGVCPYCAVGCIQLIYSKGDKIIDIEGHPDTPHTLGRLCPKGAATIQLSNNPLRPT
                     KAMYRAAGSDKWEEKPLDWMYDQIAKRYYDAREKYFIEREKNKDGEEVVVNRLDAIAS
                     LGSACIDNEECYLLSKLNRAFGIVYHEHQARV"
     gene            complement(2837..3172)
                     /locus_tag="VNU03_24705"
     CDS             complement(2837..3172)
                     /locus_tag="VNU03_24705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77747.1"
                     /translation="MTEPTESPDDSAGDASRKKGFRSRKREDHALLLIRDLLGCLDDM
                     PRARELMRELSRYYDPVLGGAILEVRHQRAIVEAIEAGRAEEARALIQERYDLYVRDR
                     AHLGRGEEA"
     gene            complement(3169..4020)
                     /gene="truA"
                     /locus_tag="VNU03_24710"
     CDS             complement(3169..4020)
                     /gene="truA"
                     /locus_tag="VNU03_24710"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWP77748.1"
                     /translation="MGGGRPAPQGRRAQRHPDRGAADPLTPLRAVRLTLTYDGTDLHG
                     WQVQPGLPTVQGLLIAAAERVLGAPVKITGASRTDAGVHALRQVASLAISSPMPPAAL
                     QRALNALLPAAVRVMAAREAPAGFDARRWARGKRYVYLIDRGPQADPFRRGHAWHVPF
                     ALDAAAMAAGLRAVRGKHDFSAFCAAAGRDRTPTCTVRSARVVTRRHLLAVCLSADSF
                     LHHMVRNIVGSLVEIGRGAHPPGWIAELLDGRDRTRAGPTAPPEGLFLVRVLYEPIEM
                     PPAGPPR"
     gene            complement(3945..4949)
                     /locus_tag="VNU03_24715"
     CDS             complement(3945..4949)
                     /locus_tag="VNU03_24715"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWP77749.1"
                     /translation="MASGVVVAVVGVTGAVGQTTLKILEERKFPVRALRPFASARSAG
                     KTVTFKGETIRVEAVGPDSFKGVDIALFSAGSAQSKEYAPFAVKAGAVVVDKSNAFRM
                     DPHVPLVVPEINPHAVKGHRGILACPNCTTIVTVMPLKPLHEAGRLRRVVATSYQAVS
                     GAGVNGVEELRSQTLAWARGEAIVPRFFQHQIAFNVIPQVDKFGDDGYTGEEIKLVNE
                     VRKILELPDLPVSPTTVRVPVFTAHSVAVNAETERKVSRERAREIFAGFPGLKLWDEP
                     AENRYPMPVLVEGQDDCYVGRIREDLSQPNALNFWVVGDQLRKGAALNGIQIAELLIR
                     "
     gene            complement(4980..6074)
                     /gene="leuB"
                     /locus_tag="VNU03_24720"
     CDS             complement(4980..6074)
                     /gene="leuB"
                     /locus_tag="VNU03_24720"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWP77750.1"
                     /translation="MATYKIAVLPGDGIGQEVTPQAQRILQVVGRAAGIGFEFEPALV
                     GGSAIDATGRPLPPASLALCQAAHAILFGSVGGPKWDNLPQEQRPERGLLAIRKELDL
                     FANLRPATCFPMLVDASPLKRSVVEGTDLMVIRELTGGLYFGEPRGREDFADGGARAV
                     NTMAYTSREIERVARAAFDVAMKRRKRLASVDKANVLVVSQLWREVVTRVGKDYPQVA
                     LEHVLVDNCAMALVHKPTHFDTIVTENTFGDILSDEAAILAGSMGMLPSASLGGTGGK
                     KGLYEPVHGTAPDIAGQGVANPIAAILSAAMLLRYSLDRGPDADRVDAAVLKVLEQGH
                     RTRDIHSAGMKLVGTVEMGDLIVSQVEKSY"
     gene            complement(6083..7630)
                     /locus_tag="VNU03_24725"
     CDS             complement(6083..7630)
                     /locus_tag="VNU03_24725"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWP77751.1"
                     /translation="MDRVLIFDTTLRDGEQAPGFSMNTGEKLEMARQLARLNVDVIEA
                     GFPISSDEDFEATREVARQVGALEGAPIICGLSRVGLMDIDRCWEAVKYARRPRIHTF
                     VATSDIHLKYKLRKSRAEVLKASVEAVKHARAYCGDVEFSPEDASRSDFDYMCDVLEA
                     VIDAGAGTINIPDTVGYAIPEEWAQRIARIRERVRNSAKAVISVHCHNDLGQAVANSV
                     AAIRVGARQVECAVNGIGERAGNASLEEIVMALHTRKDFFGVETRVRTEEIFKTSRLL
                     SHITGVHVQPNKAIVGENAFAHEAGIHQDGVLKEKLTYEIMRPQDIGRATNKLVMGKH
                     SGRHALAARLQDLGFDLDEGALAKAFKRFKDLADRKKEIFDDDLISIVKDDMAQVPET
                     YTLDYLHSISGTGIIPSATVRLKKEEQVFQDSGVGDGPVDAVLNAIDAITGVKGRLQD
                     YQLRAVTSGKDALGEVSVKVDFDGTLVFGKGTSTDVIEASARAYINAVNRLVYPVKGR
                     DVQEVGP"
     gene            complement(7632..>7945)
                     /locus_tag="VNU03_24730"
     CDS             complement(7632..>7945)
                     /locus_tag="VNU03_24730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77752.1"
                     /translation="IPRWLGAVIAAGTYLVAILMVTTFRYYSFKEIDFARRRPAGVLV
                     LVVLGVLIVATHPQWFLFLLFSAYVLSGPTRPLWARRREPAVAAIEREQGDPHDRERR
                     V"
BASE COUNT         1113 a         2704 c         2778 g         1350 t
ORIGIN      
        1 ccccatgggc gcgagatcgt tccgcttgtc gggcacgctc cagatcttgg ccacgggatt
       61 gttctgctgc ttcgagagca ggttcttggt cggggactcg atcggctcgt agtgctccgg
      121 gaacggcccg tcgttgagcg ggccgaagaa ggcgcccacc aggtccgccc gcatgatgaa
      181 gggcttgtcc ccgttcttgg cgtcgggggc gaggaaggcg ttgaagtcgg gcacgtcgag
      241 ccccacccac ttgcccttct tgtcgggctc ctgcccgggg gcggccgggt cccaccagat
      301 gagcttcttc ttctcgctcc acggcttgcc ctgcaggtcg gcggatgcgc ggttgtagag
      361 gatgcggcgg ttggccggcc acgcgaagcc ccagccgtgc gcgaggtagt ccttgtcgtc
      421 ggccggcttg cgcaggttgg ccttgttgat cagcttgccg tcggggcctt ccaccgtgac
      481 cccggtgtag atccagcacc cgccggcggt cttgccgtcg tcggtcatgt gggcgaagga
      541 gttgagggcc tgccccggct tgtagacgac cttgccgtcc ttgtcggtga tctcctgggt
      601 cgcgtagccg ttgatctcct tcagcaccag ctccatgttc ggctccttcg gatcgtcgcc
      661 gtactggaag ctggcggcca ggaagccctg gtcgcgctcc ttcttggagc ccgcgtacat
      721 cttctgcagc cgcttggcca ggctcacgaa aaactccgcg tcggaggtga cgtcccccgg
      781 cggcttcacc gcacgctcgt gccactggat cagccgcatg gtgttggtga ggctgccgtc
      841 cttctcggcc gccggcgcgg cgggcaggaa gaacacctcg gtcttgatgt ccttcgccac
      901 cgcacccggc gccttccaga actccgcggt ctcggtgacg aagatgtcct tgaccacgag
      961 ccagtcgagc ttgccgagcg cggcccgggc cagcttggcg ttcgggccgc ccaccgcggg
     1021 attctgcccg tcggcgagga agcccttgat gacgcccttg tacatgtcga cgaacatgtg
     1081 ctggtggctg taggcgtccg cggaggcgcg cttgggcagc cactggtagc cgtagtcgtt
     1141 ctcgaccttc gcggcgtcgc cgtagaaggc cttcatgagg ctgaccatga acttcggcag
     1201 gttgacccag aagccaccct tgggcgtgac cttctcgaga tacgtcttga gatccggatg
     1261 ggcgtcgcgc agcggcatcg ggagatagcc gggcagctcg tggtagagca gctcgaggtc
     1321 ggtcgcgccc tgcacgttgg cgtggccgcg cagggcgacc accccgcccc ccggccgccc
     1381 gatgttaccg agcaggatct gcagcatgca gagggcgcgg atgttctgga ccccgttggt
     1441 gtgctgggtc aggttcagcg cgtaggtgat cgacccggtc ttctccggcc cgctgttctc
     1501 gcagtagagc ttggcgacct ggacgaactt ctccttggga atgccgcaga cgcgctccac
     1561 catctccggg gtgtagcggc tgtagtgtcg cttcatgagc tggaagacgc tgctcggatc
     1621 gcgcagggtc aggtcgcgct tgggattgcc ctccccgtcg agctggtact tccacgaatc
     1681 ccgcttgtag gtctcggcca gatggggatc gacgccgccc ttgccggtcg actcgaggcc
     1741 ggtgaagagg ccgcccccgt cggtggcggt cttgaacgcg ggatcgatga ggaaggaggc
     1801 gttggtgtag gggacgacgt agtccttgaa gtagaggtta ttctggatgg cgtagctgat
     1861 cagcccgccg aagaacgcga tgttggtgcc cgaccgcagg ggcacgtgga cgtcggccac
     1921 cgcgctggtg cgggtgaacc gcggatccac gtggatgaac ttggcccccc gctccttcgc
     1981 cttcatcacc cactggaatc cgaccgggtg attctccgcg aagttgctgg tggccatgat
     2041 gacatccgag ttctggatgt cgatcaggtt cgtcgtcatc gcgccccgcc cgaacgtggt
     2101 ccccagagct gggaccgtgg cggagtgtca gactcgggcc tgatgctcgt ggtagacgat
     2161 gccgaaggcg cggttgagct tgctgaggag gtagcactcc tcgttgtcga tgcacgccga
     2221 gccgagcgag gcgatcgcgt cgaggcggtt gaccaccacc tcctcgccgt ccttgttctt
     2281 ctcccgctcg atgaagtact tctcgcgcgc gtcgtagtag cgcttggcga tctggtcgta
     2341 catccagtcg agcggcttct cctcccactt gtccgagccc gccgcgcggt acatcgcctt
     2401 ggtcggccgc agcgggttgt tggagagctg gatggtggcc gcgcccttgg ggcagaggcg
     2461 accgagggtg tgcggggtgt cgggatgccc ctcgatgtcg atgatcttgt cgcccttgga
     2521 gtagatgagc tggatgcacc cgacggcgca gtatggacag acgccggggt gcaccttggc
     2581 ttccttgatc ttgagcgtgg tggccgccgc ctggaccggc ttcaggtcga gggtggcggt
     2641 gccgaccgcg acgcccgctc cggtggaccc cgcgtacttg aggaaatcgc gccgcgagag
     2701 actcatggcg acacctcgct gtacctcgtt ggtgagcgat aagctttcgg ctggtgggcc
     2761 agcaacttgc gcacgattct agggactggt cggaccatcg gtcaagggaa tctttaccgg
     2821 ggatatgcgc gctgcgtcac gcctcctccc ctcgccccag gtgagcccgg tcgcggacgt
     2881 agagatcgta gcgctcctgg atcagggccc gggcctcctc ggcccgcccg gcttcgatcg
     2941 cctcgacgat ggcgcgctgg tggcggacct cgaggatggc gccccccagc accgggtcgt
     3001 agtagcggct cagctcccgc atgagctcgc gggcgcgcgg catgtcgtcg aggcacccca
     3061 gcaggtcccg gatgagcagg agggcgtggt cttcccgctt ccgcgagcgg aatcccttct
     3121 tccgcgacgc gtcgcccgcg gaatcgtcgg gactctcggt gggctcggtc accgcggcgg
     3181 cccggccggc ggcatctcga tcggctcgta cagcacgcgg acgaggaaga ggccctccgg
     3241 cggagcggtg gggccggcgc gggtccggtc gcggccgtcc aggagctcgg cgatccagcc
     3301 gggaggatga gccccccggc cgatctcgac gaggctgccc acgatgttcc ggaccatgtg
     3361 gtgcaggaag ctgtcggcgg agagacacac cgcgaggagg tgacgccggg tcaccacgcg
     3421 ggccgagcgc acggtgcagg tcggcgtccg gtcccggccg gccgccgcgc agaacgccga
     3481 gaagtcgtgc ttgccgcgca ccgcccggag ccccgccgcc atggccgccg cgtcgagcgc
     3541 gaaggggacg tgccacgcgt gcccgcgacg gaagggatcg gcctgcgggc cgcggtcgat
     3601 caggtagacg tagcgcttgc cgcgggccca gcgtcgcgcg tcgaagccgg cgggggcctc
     3661 gcgcgcggcc atcacgcgga ccgccgcggg cagcagcgcg ttgagcgcgc gctggagcgc
     3721 cgcgggcggc atcggcgacg agatggccag gctcgccacc tgccggaggg cgtgcacgcc
     3781 cgcgtcggtc cggctcgcgc cggtgatctt caccggcgcg cccagcaccc gctccgccgc
     3841 cgcgatgagc agcccctgca cggtgggcag ccccggctgc acctgccagc cgtgcaggtc
     3901 ggtgccgtcg tacgtgaggg tcaggcggac cgcgcggagc ggggtcagcg gatcagcagc
     3961 tccgcgatct ggatgccgtt gagcgcggcg cccttgcgga gctggtcgcc caccacccag
     4021 aagttgagcg cgttcggctg ggagaggtcc tcgcggatgc gacccacgta gcagtcgtcc
     4081 tgaccttcca ccagcaccgg catcgggtag cggttctcgg cgggctcgtc ccagagcttg
     4141 agaccgggga atccggcgaa gatctcgcgc gcccgctcgc ggctcacctt gcgctcggtc
     4201 tccgcgttga ccgccaccga atgggcggtg aagacgggga cccgcaccgt ggtgggcgag
     4261 accggcaggt cgggcagctc gaggatcttg cgcacctcgt tgaccagctt gatctcctcg
     4321 ccggtgtaac cgtcgtcccc gaacttgtcg acctggggga tcacgttgaa ggcgatctgg
     4381 tgctggaaga agcgggggac gatggcctcg ccgcgcgccc acgccagcgt ctggctccgc
     4441 agctcctcca ccccgttgac cccggcgccg gagaccgcct ggtagctggt cgccaccacc
     4501 cggcgcagcc gcccggcctc gtgcagtggc ttgagcggca tcacggtgac gatcgtggtg
     4561 cagttcgggc acgcgaggat gccccggtgt cccttcaccg cgtggggatt gatctccggc
     4621 acgaccagcg gcacgtgggg atccatgcgg aaggcgttgg acttgtcgac cacgacggct
     4681 cccgccttca ccgcgaacgg cgcgtactcc ttcgactgcg cggagcccgc cgagaagagc
     4741 gcgatgtcca cccccttgaa ggagtccggg ccgaccgcct ccacccggat cgtctccccc
     4801 ttgaaggtga cggtcttgcc cgcggagcgg gccgaggcga acgggcgcag cgcccgcacc
     4861 gggaacttgc gctcctccag gatcttgagc gtggtctgcc ctaccgctcc ggtcacgccg
     4921 acgaccgcca ccaccacccc tgaggccatt gctcgctgtc tcctgtctat ctgattggac
     4981 tagtacgact tctcgacctg cgacacgatc aggtcgccca tctcgacggt gcccaccagc
     5041 ttcattcccg cggagtggat gtcccgcgtg cggtggccct gctcgagcac cttcaggacc
     5101 gccgcgtcga cccggtccgc gtccggcccg cggtcgaggg agtaccgcag cagcatggcg
     5161 gcggagagga tcgccgcgat cgggttcgcc accccctgcc ccgcgatgtc gggcgcggtg
     5221 ccgtgcaccg gctcgtagag gcccttcttt cccccggtgc cgccgaggct cgcggacggg
     5281 agcatgccca tcgagccggc caggatggcg gcctcgtccg acaggatgtc gccgaaggtg
     5341 ttctcggtga cgatcgtatc gaagtgggtg ggcttgtgga cgagggccat cgcgcagttg
     5401 tcgacgagca cgtgctcgag cgccacctgg ggatagtcct tgcccacccg ggtcacgacc
     5461 tcccgccaca gctgcgagac cacgaggacg ttggccttgt ccaccgaggc gagccgcttg
     5521 cgccgcttca tcgccacgtc gaaggcggcg cgggccaccc gctcgatctc gcgcgaggtg
     5581 taggccatgg tgttcaccgc ccgcgcgccg ccgtccgcga agtcctcgcg cccgcgcggc
     5641 tcgccgaagt agaggccgcc ggtcagctcg cggattacca tcaggtcggt gccctcgacc
     5701 accgagcgct tgaggggcga ggcgtcgacc agcatcggga agcaggtggc ggggcggagg
     5761 ttggcgaaga ggtcgagctc cttgcggatg gcgaggaggc cgcgctcggg ccgctgctcc
     5821 tggggcaggt tgtcccactt gggcccgccc accgagccga acaggatggc gtgcgcggcc
     5881 tggcagaggg ccaggctggc gggcggcagg ggccggccgg tcgcgtcgat ggccgagccg
     5941 cccaccagcg cgggctcgaa ctcgaacccg atgccggcgg cccgcccgac cacctggagg
     6001 atgcgctgcg cctgcggggt cacctcctgc ccgataccgt cgcccggcag taccgcgatc
     6061 ttgtacgtgg ccatgacggg ccctaggggc cgacctcctg gacgtcgcgc cccttgaccg
     6121 ggtacacgag ccggttcacc gcgttgatgt aggcccgggc gctcgcctcg atcacgtcgg
     6181 tcgaggtgcc cttgccgaag acgagcgtgc cgtcgaagtc gaccttgacc gagacctcgc
     6241 ccagcgcgtc cttgcccgac gtgaccgcgc gcagctggta atcctggagc cggcccttca
     6301 ccccggtgat ggcgtcgatc gcgttcagga ccgcgtccac cggcccgtcg ccgacccccg
     6361 agtcctggaa gacctgctcc tccttcttga gccgcaccgt ggccgacggg atgatcccgg
     6421 tgccgctgat cgagtgcagg tagtcgaggg tgtaggtctc gggcacctgc gccatgtcgt
     6481 ccttgacgat cgagatcagg tcgtcgtcga agatctcctt cttccggtcc gcgaggtcct
     6541 tgaagcgctt gaaggccttg gcgagggcgc cctcgtcgag atcgaagccc aggtcctgga
     6601 gccgcgcggc cagcgcgtgg cggccggagt gcttgcccat cacgagcttg ttggtggcgc
     6661 gcccgatgtc ctggggccgc atgatctcgt aggtgagctt ctccttgagc accccgtcct
     6721 ggtggatgcc cgcctcgtgg gcgaaggcgt tctcgcccac gatggccttg ttgggctgca
     6781 cgtgcacccc ggtgatgtgg gagagcagac gcgaggtctt gaagatctcc tcggtgcgca
     6841 cccgggtctc gaccccgaag aagtccttgc gggtgtggag cgccatcacg atctcctcga
     6901 gggaggcgtt gccggcccgc tccccgatcc cgttgaccgc gcactccacc tggcgcgcgc
     6961 cgacgcggat cgcggccacc gagttggcca ccgcctgccc gaggtcgttg tggcagtgaa
     7021 ccgagatgac cgccttggcg ctgttccgga cccgctcccg gatgcgcgcg atacgctggg
     7081 cccactcctc gggaatcgcg tagccgacgg tgtccgggat gttgatggtg ccggcccccg
     7141 cgtcgatgac cgcctccagc acgtcgcaca tgtagtcgaa gtcggagcgg gaggcgtcct
     7201 cgggcgagaa ctccacgtcg ccgcagtagg cgcgcgcgtg cttgaccgcc tcgaccgacg
     7261 ccttcagcac ctcggcccgg ctcttgcgca gcttgtactt gaggtggatg tcggatgtgg
     7321 ccacgaaggt gtggatgcgc ggccgccgcg cgtacttcac cgcctcccag caccggtcga
     7381 tgtccatcag cccgacccgg ctgagcccgc agatgatcgg ggcgccctcg agcgcgccga
     7441 cctggcgggc gacctcccgg gtggcctcga agtcctcgtc ggaggagatc gggaagccgg
     7501 cctcgatcac gtccacgttg aggcgcgcga gctgccgcgc catctcgagc ttctccccgg
     7561 tgttcatcga gaacccggga gcctgctcgc cgtcccgcag cgtggtgtcg aagatgagca
     7621 cgcggtccac gtcagaccct ccgctcgcgg tcgtgcgggt cgccctgctc ccgctcgatc
     7681 gcggcgacgg ccggctcccg gcgacgcgcc cacagcgggc gggtcgggcc gctcagcacg
     7741 taggcggaga agagcaggaa caggaaccac tggggatggg tggccacgat cagcaccccc
     7801 agcaccacca gcaccagcac cccggcgggc cggcggcggg cgaagtcgat ctccttgaag
     7861 ctgtagtagc ggaaggtggt caccatcagg atcgccacca gataggtccc ggccgcgatc
     7921 accgccccga gccagcgggg aatct
//
LOCUS       DATMCV010001080         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_194286, whole genome shotgun sequence.
ACCESSION   DATMCV010001080 DATMCV010000000
VERSION     DATMCV010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194286"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VNU03_24735"
     CDS             complement(<1..465)
                     /locus_tag="VNU03_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP77753.1"
                     /translation="MDLAVEPGTVHAVIGPNGAGKTTLFNCITGVVPPDRGRIVFDGH
                     DIAGLRPDQITGRGLVRTFQIARGLPRLSVLENLLLYGTHQPGERMLPALLRPAAALR
                     REEALIARAVAIARRLNLAGVIRNPASDLSGGQKKLLEIGRALMSEPKLMLLD"
     gene            complement(537..1358)
                     /locus_tag="VNU03_24740"
     CDS             complement(537..1358)
                     /locus_tag="VNU03_24740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77754.1"
                     /translation="MLDSYGESVLIFLGINMVLALSLYLPISAGQLSLGHGGFMAIGA
                     YVTVICTSWLGWPLVAGLAGGGLAAALAGAVVGFPALRIKGIYLIILTMGFGEIVRIF
                     FLNFEPTGGASGIGGIKHLTTLPLVMGVAALMLLCLMRFRGARIGRALRAVQEDEVAA
                     EAMGVNLTRVKVAAFALGALGAGIGGGLYAHQALFIDPAQFGFFRSAEIFLIVVLGGL
                     GTYWGPVVGAAVVTLLPELLRFVQDWRMTVFGSLLVLCAVLRPAGLLSGRRRPAV"
     gene            complement(1368..2243)
                     /locus_tag="VNU03_24745"
     CDS             complement(1368..2243)
                     /locus_tag="VNU03_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77755.1"
                     /translation="MLLQQLVNGLMLGSTYSLIAIGYALIFGVLGLVHLAHGEVFMMG
                     ALIGLQIVLWTQAGPVAALVGALLGTAVLGVLIELIAFRPIRARNGPIFAPMVSTIGV
                     GLILQEVATKAFGGEQVGFPHRLEPVTWRVGPVTISSVQLLILGTSLVLMLALHLWVT
                     RTRMGMAMRASAESTLTARLLGISTDRVILVTFAVASALAGAAGVLVGLSFNAISPFM
                     GIDMGVKGLAIMLLGGLGNIYGAMVGGLLLGAIEVLSVAYLASSYRDAFVFGVMILIL
                     LFRPQGLFGVGRVDR"
     gene            complement(2260..>2592)
                     /locus_tag="VNU03_24750"
     CDS             complement(2260..>2592)
                     /locus_tag="VNU03_24750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77756.1"
                     /translation="TVNDFWTDNPDPAVAKWVAEYKQRSPTPPSNAAALMYDTLDIVR
                     ACAEKSGLSGKPDDLEKDRERMRDCLARVKDYKAPVTGSTTFNGDGDALRQPVVLVVR
                     KGKFESVR"
BASE COUNT          437 a          897 c          940 g          318 t
ORIGIN      
        1 gtcgagcagc atcagcttgg gctcgctcat caaggcacgt ccgatctcga gcagcttttt
       61 ttgcccgccc gacagatcgg aggccgggtt gcggatgacg ccggcgagat tgagccgccg
      121 ggcgatggcg acggcgcggg caatcagggc ctcctcgcgc cgcagcgccg ccgccggccg
      181 cagtagcgcc ggcagcatgc gctcgcccgg ctggtgcgtg ccgtagagca gaaggttctc
      241 cagcacggag agccgcggca agccgcgcgc gatctggaac gtgcgcacca ggcccctgcc
      301 ggtgatctga tcgggcctca gaccggcgat gtcgtgccca tcgaagacga tgcgcccgcg
      361 gtccggcggc accacaccgg tgatgcaatt gaagagcgtg gtcttgccgg ctccgttcgg
      421 cccgatcacc gcgtggacgg tccccggctc cacggccagg tccaccccgg ccagggcctg
      481 gtagccgccg aagcgcttgg cgagcccgtc tacgcgtagc acggtcgggg tcgtcctcac
      541 accgccgggc gccggcgtcc cgagagaagg ccggcgggcc ggaggaccgc gcagaggacc
      601 aagagggagc cgaacacggt catccgccag tcctgcacga agcgcagcag ctcgggcagg
      661 agcgtgacca ccgccgcgcc cacgaccggg ccccagtagg tgccgaggcc gccgagcacc
      721 acgatgagga agatctcggc ggagcggaag aagccgaact gcgcggggtc gatgaagagc
      781 gcctggtggg cgtagagccc gccgccgatc ccggccccga gggccccgag ggcgaaggcg
      841 gccaccttca cccgcgtgag gttcaccccc atggcctcgg cggccacctc gtcctcctgc
      901 accgcgcgga gcgcgcggcc gatgcgggcc ccgcggaagc gcatcaggca gagcagcatc
      961 agcgcggcga cgcccatcac gaggggcagg gtggtgaggt gcttgatgcc gccgatgccg
     1021 gaggccccgc cggtgggctc gaagttgagg aagaagatgc gcacgatctc gccgaacccc
     1081 atcgtcagga tgatgaggta gatccccttg atgcgcaggg cggggaagcc gacgacggcg
     1141 ccggcgagcg ccgcggccag gccgccgccc gcgagccctg ccacgagcgg ccagccgagc
     1201 cagctggtgc agatcacggt gacgtaggcg ccgatcgcca tgaagccgcc gtggcccagc
     1261 gagagctggc cggccgagat gggcaggtag agcgagagcg ccagcaccat gttgatgccg
     1321 aggaagatca ggaccgactc cccgtacgag tcgagcacgg gcgcgctcta gcggtccacc
     1381 cgtccgaccc cgaagaggcc ctggggtcgg aagaggagga tcaggatcat caccccgaag
     1441 acgaacgcgt cgcggtacga cgacgcgaga taggcgacgc tcagcacctc gatggcgccc
     1501 agcagcaggc cgccgaccat cgcgccgtag atgttgccga ggccgcccag cagcatgatc
     1561 gcgagcccct tcacgcccat gtcgatgccc atgaacgggg agatcgcgtt gaacgagagg
     1621 ccgaccagca ccccggccgc gccggccagg gccgaggcca ccgcgaaggt gacgaggatg
     1681 acgcggtcgg tggagatgcc gagcagccgc gcggtgaggg tgctctcggc gctcgcgcgc
     1741 atggccatgc ccatccgggt gcgggtgacc cagaggtgca gggccagcat caggaccagc
     1801 gaggtgccga ggatcaggag ctgcaccgag ctgatcgtga ccgggccgac gcgccaggtg
     1861 accggctcga gccgatgcgg gaagccgacc tgctccccgc cgaacgcctt ggtcgcgacc
     1921 tcctggagga tcaggcccac cccgatggtg ctgaccatcg gggcgaagat cggcccgttg
     1981 cgggcccgga tcgggcggaa cgcgatcagc tcgatcagga cgccgagcac cgcggtgccg
     2041 aggagcgcgc cgaccagggc cgcgaccggc cccgcctgcg tccacagcac gatctgcagc
     2101 ccgatcagcg cgcccatcat gaagacctcg ccgtgggcca ggtgcacgag cccgagcacg
     2161 ccgaagatca gcgcgtagcc gatggcgatg agcgagtacg tgctcccgag catcagcccg
     2221 ttgacgagct gctggagcag caccgggcgc gcccgcccgc taccggacgg actcgaactt
     2281 gcccttgcgc accaccagca ccaccggctg gcggagggcg tcgccgtcgc cgttgaaggt
     2341 ggtgctgccg gtcaccggcg ccttgtagtc cttcacccgg gcgaggcagt cgcgcatccg
     2401 ctcgcggtcc ttctcgaggt cgtcgggctt gccgctgagg ccgctcttct ccgcgcaggc
     2461 gcgcacgata tcgagcgtat cgtacatgag cgcggccgcg ttgctgggcg gggtggggga
     2521 gcgctgcttg tactcggcca cccacttggc gaccgcgggg tcggggttgt cggtccagaa
     2581 gtcattgacc gt
//
LOCUS       DATMCV010001081         3729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_194470, whole genome shotgun sequence.
ACCESSION   DATMCV010001081 DATMCV010000000
VERSION     DATMCV010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194470"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VNU03_24755"
     CDS             <1..1341
                     /locus_tag="VNU03_24755"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWP77757.1"
                     /translation="PEYPRMQRLAENIAEVRQQLRAEVQRFVESVRREYLAALQNETE
                     IQKLLDAQRGLARKLDGQMARYNLLRREVDTSRDLYAALSTRLKETQVSASLLLSNIS
                     VVDRADVPVKRSGPRTGLNLAVGCLVGLVGGVILAFLFEYLDTSIRDPREIETVLRVP
                     TLGLVPTRSALQAHLDGGPLGSGVGPSGGHGAFALVSHQATSSILAEAFRNLRTSVVY
                     ATPDRPPKTMLVTSLQQQDGKTSVSTNCAITLAQLGMGDVLLVDADMRHPDLHGILGV
                     PQTPGLSDLLVGGIGVAEVIRPTRIPGLFVIPAGPVPTNPTELLFSPRFTQALAALGE
                     RFAHIVIDTPPMLGVSDTLVLAPRVEGVILVLRHGHTGRDAARRAVQMLGSVRARLLG
                     VVLNHADVRTTAAGYQYYHREPGPAASMWPDWTPRGRAASRGLSQGLDKDLGVL"
     gene            1338..3263
                     /locus_tag="VNU03_24760"
     CDS             1338..3263
                     /locus_tag="VNU03_24760"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HWP77758.1"
                     /translation="MRRPFVTTRRAGVLLADALLAVAGNYLAFWLRFDGVIPPAYWIL
                     WLQTIPCLVLIRGLTFVSFHLYDSVWQYVGLRDLRAIAASVAASSVVFCLLIRGVLDL
                     RAYPRSVFVVDLVLVVFFMGAVRVSWRLAREYRANARTKSVLIFGAGDAGEAIVREIL
                     NGGEHGYHPVGFVDDDPAKVGRRIHGVPVLGTQADLPRIIAETNPSEVLVAIARATPQ
                     MLRQVVKALESHKLPIKAVPHLRDVLDGRAGLHEVRDLAVTDLLGRAPVGLDVETVKH
                     LVEGKRVLVTGAGGTIGSELSRQIAGLGPKSLVLFERYENALWAVERSVAGLRPSGRV
                     YSVIADVTDQQRVDEVLADHRPELILHAAAHKHVPLMELNPCEAIKNNVKGTRIVGEA
                     AVRHGVERFVLISSDKAVNPSSVMGATKRVAELLAMALAERGPTGFVVVRFGNVMGSN
                     GSVIPLFLEQIHGGGPVTVTHPDMRRYFMSIPEAVQLVLQAAALDENGALYVLDMGEE
                     IRVVDMARNLIRLSGFIPDREIPIVFTGIRPGEKLSEELVAADEAIEPSRLEGVRRVR
                     SAREMSAASLARDVADLEHFAETGATGAVMQQLGSMVALFRPVLPAKPEVANGRPVPP
                     VEMTKPAAVVGEARGAA"
     gene            3263..>3729
                     /locus_tag="VNU03_24765"
     CDS             3263..>3729
                     /locus_tag="VNU03_24765"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWP77759.1"
                     /translation="MGSVRWSGWPKRAFDLVAASVGLIALAPLLGVTALAIRLVLGGP
                     VIFAQRRPGRHGRPFVLYKFRTMSGASAGDGRLLPDRERLTRLGRLLRQTSLDELPEL
                     VNVIRGDMSLVGPRPLLMEYLPRYTREQARRHDVRPGITGWAQLNGRNTLPWER"
BASE COUNT          504 a         1280 c         1368 g          577 t
ORIGIN      
        1 cccgagtatc cgcggatgca gcggctggcc gagaacatcg cggaggttcg ccagcagctg
       61 cgcgccgagg tccagcgatt cgtcgagtcg gtccgccggg agtacctggc cgcgctccag
      121 aacgagaccg agatccagaa gctcctggac gcgcagcgcg ggctggcgcg gaagctggac
      181 ggccagatgg cgcggtacaa cctcctgcgg cgagaggtgg acacgagccg ggatctctac
      241 gcggcgctgt ccacccggct gaaggagacc caggtctccg cctcgctcct cctctcgaac
      301 atctcggtgg tggaccgggc cgacgttccc gtgaagcgct ccggcccgcg taccgggctc
      361 aatctggcgg tcgggtgcct ggtgggcctg gtcggcggcg tgatcctcgc gttcctcttc
      421 gagtacctgg atacgagcat ccgcgatccc cgcgagatcg agaccgtgct ccgcgtgccc
      481 accctcgggc tggtgccgac gcgctccgcg ctccaggccc atctcgacgg cgggcctctc
      541 ggatcgggcg tggggccctc gggtggccac ggcgccttcg cgctcgtctc gcaccaggcc
      601 accagctcga tcctcgcgga ggccttccgg aatctccgaa cgagcgtggt gtacgccacc
      661 ccggatcgtc cgcccaagac gatgctggtg acctcgctgc agcagcagga cggcaagacc
      721 tcggtctcga cgaactgcgc gatcaccctg gctcagctcg ggatgggcga tgtcctcctg
      781 gtggacgcgg acatgcggca tcccgacctg cacgggatcc tcggcgtccc ccagacgccc
      841 ggcctgtccg atctgctcgt ggggggcatc ggggtggcgg aggtcatccg gccgacccgg
      901 atcccggggc tcttcgtgat cccggcgggc ccggtgccga ccaatccgac ggagctgctg
      961 ttctcgccgc gattcaccca ggccctcgcc gcgctcgggg agcggttcgc tcacatcgtg
     1021 atcgacaccc cgccgatgct gggggtcagc gacaccctgg tgctcgcgcc gcgggtggaa
     1081 ggggtgatcc tggtcctccg tcacgggcac accggtcggg acgccgcgcg gcgtgcggtg
     1141 cagatgctcg gatcggtccg cgcgcggctg ctgggggtcg tgctcaatca cgcggacgtt
     1201 cgaaccaccg cggccggcta ccagtactac catcgcgaac ccggccccgc cgcgtcgatg
     1261 tggccggact ggacgccccg cgggcgcgcg gcatcgcgag ggttgagtca gggtctcgac
     1321 aaggatctcg gcgtcctttg agacgaccgt tcgtcacgac ccgccgcgcc ggggtgctcc
     1381 tcgcggatgc gctcctggcc gtcgccggga actaccttgc cttctggctc cggttcgacg
     1441 gggtgatccc gcccgcctac tggatcctct ggctgcagac gatcccctgc ctcgtgctga
     1501 tccgggggct caccttcgtg tcgtttcacc tctacgactc ggtctggcag tacgtgggac
     1561 ttcgagatct gcgggccatc gcggccagcg tggccgcgag cagcgtggtg ttctgcctgc
     1621 tgattcgcgg cgtgctggac ctgcgcgcct acccgcgctc ggtgttcgtc gtcgacctgg
     1681 tgctcgtggt cttcttcatg ggggcggtgc gggtgagctg gcgcctggcg cgggagtacc
     1741 gggcgaacgc ccggacgaag tcggtcctga tcttcggggc gggtgacgcg ggagaggcga
     1801 tcgtccgcga gatcctcaac ggcggggagc acggctatca cccggtcgga ttcgtggacg
     1861 acgacccggc caaggtcggc cggcgcattc acggggtccc ggtcctgggc acccaggcgg
     1921 acctgccgcg gatcatcgcg gagacgaacc cgagcgaggt gctggtcgcg atcgcccgcg
     1981 ccaccccgca gatgctccgg caggtggtga aggcgctcga gtcgcacaag ctgcccatca
     2041 aggcggtgcc gcacctccgc gacgtgctcg acggccgcgc ggggctccac gaggtccggg
     2101 acctcgcggt gacggatctg ctggggcgag cgccggtcgg gctcgacgtg gagacggtga
     2161 agcatctcgt cgaggggaag cgggtgctgg tgaccggcgc gggcgggacg atcgggtccg
     2221 agctgagccg gcagatcgcc gggctgggcc cgaagagcct cgtgctgttc gagcggtacg
     2281 agaacgcgct ctgggcggtg gagcgctcgg tggccgggct ccggcccagc ggccgcgtgt
     2341 actccgtcat cgcggacgtc accgaccagc agcgggtcga cgaggtgctg gccgaccatc
     2401 gtccggagct gatcctccac gcggcggccc acaagcacgt gccgctcatg gagctcaatc
     2461 cctgcgaggc gatcaagaac aacgtgaagg gcacccggat cgtcggagag gccgcggtcc
     2521 gccacggggt ggagcggttc gtcctgatct cgagcgacaa ggcggtcaac ccctcgagtg
     2581 tcatgggggc caccaagcgg gtcgcggagc tgctcgcgat ggcgctcgcc gagcggggcc
     2641 cgaccggctt cgtggtggtg cgcttcggca acgtgatggg gagcaacggc agcgtgatcc
     2701 cactcttcct cgagcagatc cacgggggcg ggccggtcac cgtcacgcat cccgacatgc
     2761 ggcgctactt catgtcgatc ccggaagcgg tgcagctcgt gctccaggcc gccgcgctcg
     2821 acgagaacgg agcgctctac gttctcgaca tgggcgagga gatccgggtg gtggacatgg
     2881 cccggaacct gatccggctg tccggcttca tcccggaccg ggagattccg atcgtcttca
     2941 ccgggatccg gcccggcgag aagctctcgg aggagctggt ggccgccgac gaggccatcg
     3001 agccgtcgcg cctcgaaggg gtccgacggg tccgatcggc gcgggagatg tcggccgcga
     3061 gcctcgcccg tgacgtcgcc gatctcgagc acttcgccga gacgggcgcg accggggcgg
     3121 tcatgcagca gctcgggtcg atggtcgcgc tgttccggcc ggtgttgccc gcgaagccgg
     3181 aggtcgcgaa cggacgaccg gtgcctcccg tcgagatgac gaagcccgcc gcggtggtcg
     3241 gcgaggcgcg aggcgccgcg tgatggggag cgtgagatgg tcggggtggc ccaagcgcgc
     3301 cttcgatctc gtggcggcgt cggtcgggct gatcgcgctc gcgcccctgc tgggcgtgac
     3361 cgccctggcg atccggctgg tcctcggcgg cccggtgatc ttcgcgcagc gccggcccgg
     3421 ccggcacggg cggccgttcg tgctctacaa gtttcgcacc atgagcggcg cgagtgcggg
     3481 cgacggccgg ctcctgccgg atcgtgagcg actgacccgc ctgggccggc tcctccggca
     3541 gacgagcctc gacgagctgc cggagctcgt caacgtgatc cggggcgaca tgagcctggt
     3601 cggcccccgg ccgctcctga tggagtacct gccgcgctac acgcgggagc aggcccgccg
     3661 ccacgacgtg cggcccggga tcaccgggtg ggcccagctc aacggccgca acaccctgcc
     3721 gtgggagcg
//
LOCUS       DATMCV010001082         3108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_792933, whole genome shotgun sequence.
ACCESSION   DATMCV010001082 DATMCV010000000
VERSION     DATMCV010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792933"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..601)
                     /locus_tag="VNU03_24770"
     CDS             complement(200..601)
                     /locus_tag="VNU03_24770"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP77760.1"
                     /translation="MKRQNIQPASLSKRVVGGHVLYSHVVVVEGKKTIFVSGQLARGP
                     AGEVVGAGDMRAQLRQVGENIKAALAAAGATLADIVKTNTYVTDIEEFFKHVDVRMEY
                     FGALPTSTTVEVRKLAHPDLVVEVEAIAVVD"
     gene            complement(612..2186)
                     /locus_tag="VNU03_24775"
     CDS             complement(612..2186)
                     /locus_tag="VNU03_24775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77761.1"
                     /translation="MLTANDGSRSRFSSPLALLGLTLAALVLAAPMAARAQSGKAAPG
                     EMRWALHVTLASRWLDPAETEAFSTPFMVMYALHDAMLKPMPAGLTSPSLAESWTEAK
                     DHLSYTFVLRKGVRFHNGEPVTAEDVKFSWDRYKGASAKLLKDKVKEVQVVDPGQVRF
                     VLREPWPDFITFYGTTASGAGWIVPKKYVEKVGDDGFKAAPVGAGPYKVVSFKPGVEL
                     VLEAFDGYWRKAPSVKRLVMRSMPEEATRAAALRAGEVDIAYLLTGPTADSVRKTSGL
                     RLAAPLVSGAFWIELPEQWDPKSPWSDPRVRLAASHAIDRPGINQAEMLGFGRLTGNM
                     IPRIFQFAVAMEPHAYDPARSRKLLAEAGYPNGFDAGDFYPFPPYDTMGEAILGNFQT
                     VGIRSRLRTMERAAYFTAWREKKLHGVIKVITAAFGNAATRLEPYVIKGGVYAYGSVP
                     EIDDLYVQQARELDVKKREALVHHIQQIIRDKVINIPLFEQAFIWGVGPRVAEAGDGR
                     IPGFSYSAPFEDLRLK"
     gene            2246..>3108
                     /locus_tag="VNU03_24780"
     CDS             2246..>3108
                     /locus_tag="VNU03_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008317327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWP77762.1"
                     /translation="MLPTHGRYDYSNITTRPDYSWPGGRRLAVYLALNIEAFGFGVGK
                     GAAIAPPDQAQSHSVYSWRDYGNRVGIWRLFELLDELGLPAEAQMNAAVYEMAPDIPA
                     KLRARGDEILGHGLTNSDEQGHLAEDRERALIAEATAAIERHEGARPVGWMSPWLSNS
                     AVTMDLLQEAGYRYVMDWTMDDQPIWLSTRKGRILSIPYPIEVNDTRGIIWYHHTSQE
                     FAEMITDQFDEMLDQSARQPLVCPISLHPFVFGRPYRIRRLRRALQHILGHGDRVWIT
                     RPRDICAHIESL"
BASE COUNT          482 a         1071 c         1094 g          461 t
ORIGIN      
        1 cacgcctgaa ggcatcgagc agcccccgca tgcgaggatc ggaagcgctg gccggcgaga
       61 gaaagccgat ccggacgact tttcccggct gggcccaggc ggccaccgga gcggcgagga
      121 gagcgccggc caaggccccg agaagcacgc ggcggtccat ggtaggggct cccatgcgcg
      181 ctcgctctcg cgatggcggt cagtcgacga ccgcgatcgc ctccacctcc accaccaggt
      241 cggggtgggc gagcttgcgc acctccaccg tggtgctggt cggcagcgcg ccgaagtact
      301 ccatgcggac gtcgacgtgc ttgaagaact cctcgatgtc ggtgacgtag gtgttggtct
      361 tcacgatgtc ggcgagggtg gcgcccgccg cggccagcgc ggccttgatg ttctcgccca
      421 cctggcggag ctgggcgcgc atgtcgcccg ccccgaccac ctcaccggcc gggccgcggg
      481 cgagctggcc cgacacgaag atcgtcttct tgccctcgac gaccaccacg tgggagtaga
      541 gcacgtggcc gcccacgacc cgcttgctga gcgacgccgg ctggatgttc tggcgcttca
      601 tgacggcctc ctcacttgag acgcaggtcc tcgaacgggg ccgagtagga gaagccgggg
      661 atgcggccgt cgcccgcctc ggccacccgc ggccccaccc cccagatgaa cgcctgctcg
      721 aagagcggga tgttgatcac cttgtcgcgg atgatctgct ggatgtggtg gaccagcgcc
      781 tcccgcttct tgacgtcgag ctcgcgcgcc tgctggacgt agagatcgtc gatctcgggg
      841 accgacccgt aggcgtagac gccgcccttg atgacgtagg gctcgagccg cgtggccgca
      901 ttgccgaagg cggcggtgat gaccttgatc acgccgtgga gcttcttctc ccgccacgcg
      961 gtgaagtagg ccgcgcgctc catcgtgcgg agccggctgc ggatgccgac cgtctggaag
     1021 ttgcccagga tggcctcgcc catggtgtcg tagggcggga acgggtagaa gtcgcccgcg
     1081 tcgaagccgt tcggatagcc ggcctcggcg aggagcttcc tggaccgggc cggatcgtag
     1141 gcgtgcggct ccatcgccac cgcgaactgg aagatgcgcg ggatcatgtt gccggtgagc
     1201 cgcccgaagc cgagcatctc ggcctggttg atcccgggcc ggtcgatggc gtggctcgcg
     1261 gcgagtcgca cgcgcggatc ggaccagggc gatttgggat cccattgctc gggcagctcg
     1321 atccagaacg cgccgctcac caggggggcg gccagccgga ggcccgacgt cttgcggacc
     1381 gagtccgcgg tgggcccggt caggagatag gcgatgtcca cctcgccggc gcgcagcgcg
     1441 gcggcgcggg tcgcctcctc cggcatcgag cgcatgacca ggcgcttgac cgagggcgcc
     1501 ttgcgccagt agccgtcgaa ggcctccagc accagctcca cccccggctt gaacgagacg
     1561 accttgtagg ggcccgcgcc gaccggcgcg gccttgaagc cgtcgtcccc gaccttctcc
     1621 acgtacttct tcggcacgat ccagcccgca ccggacgcgg tggtgccgta gaaggtgatg
     1681 aagtcgggcc acggctcgcg caggacgaag cgcacctggc cggggtcgac gacctggacc
     1741 tccttcacct tgtccttgag cagcttcgcg ctcgcgccct tgtagcggtc ccacgagaac
     1801 ttcacgtcct cggcggtgac cggctcgccg ttgtggaagc gcaccccctt gcgcagcacg
     1861 aaggtgtagg agaggtgatc cttcgcctcg gtccaggact cggcgaggct cgggctcgtg
     1921 agcccggccg gcatcggctt cagcatggcg tcgtggagcg cgtacatgac catgaacggc
     1981 gtgctgaagg cctcggtctc cgccgggtcg agccagcggg aggcgagggt cacgtgcagc
     2041 gcccagcgca tctcgcccgg cgccgccttg ccggactgcg cgcgggccgc catcggggcg
     2101 gcgaggacga gcgcggcgag cgtgaggccg agcagcgcga gcggactgga aaacctcgag
     2161 cgggagccgt cgttggcggt tagcatggcg cataccgtag tctgaggggc cgggaaggtc
     2221 aatcgaccga cggagggacc gcgacatgct gccgacgcac gggcgctacg actactcgaa
     2281 catcaccacg cggcccgact actcgtggcc cggcggccgg cggctcgcag tgtacctggc
     2341 gctcaacatc gaggccttcg gcttcggggt aggcaagggc gcggcgatcg cgccgcccga
     2401 ccaggcgcag agtcacagcg tctacagctg gcgggactac ggcaaccggg tcggcatctg
     2461 gcggctcttc gagctgctcg acgagctggg gcttcccgcc gaggcccaga tgaacgcggc
     2521 ggtctacgag atggcgccgg acatccccgc gaagctgcgc gcgcggggcg acgagatcct
     2581 gggccacggc ctcaccaact ccgacgagca ggggcacctg gccgaggacc gcgagcgcgc
     2641 gctgatcgcg gaggcgaccg cggcgatcga gcggcacgag ggcgcgcggc cggtgggctg
     2701 gatgagcccg tggctctcca acagcgcggt caccatggat ctgctccagg aggcgggcta
     2761 ccgctacgtg atggactgga ccatggacga ccagcccatc tggctctcca cgcgtaaagg
     2821 ccgcatcctc tcgatcccgt atccgatcga ggtgaacgac acgcggggca tcatctggta
     2881 ccaccacacc tcgcaggagt tcgcggagat gatcaccgac cagttcgacg agatgctgga
     2941 ccagtcggcc cgccagccgc tggtctgccc catctcgctc caccccttcg tgttcggccg
     3001 cccgtaccgc atccgccggc tgcgccgggc gctccagcac atcctggggc acggggatcg
     3061 cgtctggatc acgcgtcccc gcgacatctg cgcccacatc gaatcgct
//
LOCUS       DATMCV010001083         3593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_525681, whole genome shotgun sequence.
ACCESSION   DATMCV010001083 DATMCV010000000
VERSION     DATMCV010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525681"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VNU03_24785"
     CDS             complement(<1..640)
                     /locus_tag="VNU03_24785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77763.1"
                     /translation="MDAAALLGRRRSNFRQLQTRRVKSAKGALAFVDAIGLCSTFYVF
                     PEGVGCLWEAVVGRPAPRWPRRSHHDAGIGLTWELKDVLPARRQVYYGKLVKGRPMLV
                     ALDLFPVLYALERGRQRARDYRREYEEGRLSATAKRIMDRLVRESPQYTRGLRAECFM
                     LEAAKTREFERAMGELQQGLWIVKTEERYEPTFSYRWDLVERWLPDEVAAGRR"
     gene            complement(646..1530)
                     /locus_tag="VNU03_24790"
     CDS             complement(646..1530)
                     /locus_tag="VNU03_24790"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWP77764.1"
                     /translation="MTTRVSTGSPKLDEMLGGGLLPGTLTVVYGATGIGKTHLGLSFC
                     DHGARADGARGIVFDMNARGDSQQHHAYAARLYGWDLRRWTHTVLPMTDPYPPPEQMQ
                     AFYCDALPWVGKLRDYQVPSGDGLEFDWNWKAQYNQALYTVRPFVYFHLGAGSRRIVV
                     DGIEPMDVPGDYIQPYIFDDLYRKLIHRDSETLGMEICMPVWQHRAFIDAHRYDHAAV
                     TTLLLVTTEETQLEHLIARKVAAGDVGAVANTIVVMGSERVGNRLGRFLCVVKHRGSA
                     KSDEIVEYRVTERGFELG"
     gene            1612..3510
                     /locus_tag="VNU03_24795"
     CDS             1612..3510
                     /locus_tag="VNU03_24795"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWP77765.1"
                     /translation="MPRRVVWYPAGSPAASLLSALPGDLEATPLPARGLPRSGPDEGA
                     VVLVDLTAGDARTAADLATRAPDLPVVALVAPGAAPAAACYAYVTTPAAPDLLAVTLR
                     NACDHARARREADTTRREMEQLNQIGVSLSAERDTDALLTLILSKAREITRSDAGSVY
                     LVEESPDGPARLRFKLAQNDSVLVPFTEFTLPIDAASVAGHVALTSSILQLDDAYVLP
                     PGSPFKINREFDERVGYRTRSMLVVPMVTPQDTVIGVLQLINCKRDASAMLGTAAAVE
                     AAVVPYPEHFRGLAASLASQAAVALENSRLYQSIQTLFEGFVQASVTAIESRDPTTSG
                     HSFRVADFTVALAAAVDRAAHGPFRDVRFSVDEMKEIRYASLLHDFGKVGVREDVLVK
                     AKKLPPGQLELIRQRGEIIRRGLELRHARRKTEWLLQHGRDAFADRCAEWDAELAAVL
                     ADLDVHLKAVAAANEPTVMPDDVSAGIRDLAGRAFLDHLGDPLTLITPEEARVLSIPR
                     GSLTPDEYRQIQSHVVHTYQFLRQIPWTKELRRIPEIARSHHEKLNGSGYPAGWRAPE
                     IPVQTRMMTISDIFDALTSRDRPYKPAVPVERALDILGQERQAGALDGELLSLFIAVR
                     PWERVDPA"
BASE COUNT          523 a         1356 c         1189 g          525 t
ORIGIN      
        1 gccgacggcc cgcggccacc tcgtcgggca gccaccgctc caccaggtcc cagcgatacg
       61 agaaggtggg ctcgtagcgc tcctcggtct tgacgatcca caggccttgc tgcagctcgc
      121 ccatcgcgcg ctcgaactcc cgggtcttgg ccgcttccag catgaagcac tccgcccgca
      181 ggccccgcgt gtactgcggc gactcacgca ccaggcggtc catgatgcgc ttcgcggtcg
      241 ccgacaggcg tccctcctcg tactcgcgtc gatagtcccg ggcgcgctgg cggcctcgtt
      301 cgagagcgta caagaccggg aagaggtcga gcgccaccag catcgggcgg cccttgacca
      361 gcttgccgta gtagacctgg cggcgggcgg gcagcacgtc cttcagctcc caggtgagcc
      421 cgatgcccgc gtcgtggtgc gagcgacgcg gccaccgggg ggcgggccgg cccaccaccg
      481 cctcccagag gcagcccacg ccctccggaa agacgtagaa ggtggagcag aggcctatcg
      541 cgtcgacgaa ggcgagtgcc cctttcgccg atttcacgcg gcgagtctgg agctgacgga
      601 agttcgagcg ccggcgcccg agcagcgcgg ccgcgtccat gccgactagc cgagctcgaa
      661 gccccgctcg gtgacgcggt actcgacgat ctcgtcgctc ttggcgctgc cccggtgttt
      721 gaccacgcag aggaagcggc cgagccggtt gcccacccgc tcgctcccca tcaccacgat
      781 ggtgttggcc accgcgccga cgtcgcccgc ggccaccttg cgggcgatga ggtgctcgag
      841 ctgggtctcc tcggtggtca ccaggagcag cgtcgtgacc gcggcgtgat cgtagcggtg
      901 cgcatcgatg aacgcccggt gctgccagac cggcatgcag atctccatgc cgagggtctc
      961 gctgtcccgg tggatcagct tccggtagag gtcgtcgaag atgtagggct ggatgtagtc
     1021 gcccggcacg tccatcggct cgatgccgtc caccacgatg cggcggcttc ccgcgcccag
     1081 gtggaagtac acgaacgggc gcaccgtgta gagcgcctgg ttgtactggg ccttccagtt
     1141 ccagtcgaac tcgagcccgt ccccgctggg gacctggtag tcgcgcagct tccccaccca
     1201 cggcagcgcg tcgcagtaga aggcctgcat ctgctcgggg ggcgggtacg gatcggtcat
     1261 gggcagcacg gtatgagtcc agcgcctcag atcccagccg tagaggcgcg ccgcgtaggc
     1321 gtggtgctgc tgcgaatccc cgcgagcgtt catgtcgaac acgatgccac gggccccgtc
     1381 cgcccgggcc ccgtggtcgc agaagctcag tcccaggtgg gtcttgccga tcccggtcgc
     1441 cccgtagacc acggtcaggg tgccgggcag caggccccca cccagcatct cgtcgagctt
     1501 cggacttccg gtggaaactc gcgtggtcat agacgaagga ttctagcggc ccgctcgcga
     1561 ggtggtcaag caaccttgac acaccgccga ggtgagtcct acgctcgata catgccacgc
     1621 cgagtggtct ggtacccggc gggttccccg gcggcctccc tgctctccgc gctccccggc
     1681 gatctcgagg cgacgccgct gcccgcgcgc gggctgccgc ggtcggggcc cgacgagggc
     1741 gcggtcgtgc tggtcgacct caccgcgggc gacgcccgca ccgccgccga cctggccacg
     1801 cgggcgcccg atctccccgt ggtggccctg gtcgcccccg gcgcggcccc ggccgcggcg
     1861 tgctacgcct acgtgacgac acccgccgcg ccggacctgc tggccgtcac gctgcgcaac
     1921 gcgtgcgatc acgcgcgggc ccgccgcgag gccgacacga cccggcgaga gatggagcag
     1981 ctcaaccaga tcggggtcag cctctccgcc gagcgcgaca ccgacgccct gctcacgctg
     2041 atcctgagca aggcccgcga gatcacccgc agcgacgccg gctccgtcta cctggtggaa
     2101 gaatcgccgg acgggccggc gcggctccgc ttcaagctgg cccagaacga ctcggtgctc
     2161 gtgccgttca ccgagttcac gctgccgatc gacgcggcga gcgtcgccgg tcacgtggcc
     2221 ctgacttcct cgatcctcca gctggacgac gcctacgtgc tcccgcccgg ctcgcccttc
     2281 aagatcaacc gcgagttcga cgagcgggtc ggctaccgca cccggtcgat gctggtggtt
     2341 ccgatggtca cgccccagga caccgtgatc ggcgtcctcc agctcatcaa ctgcaagcgc
     2401 gacgcctcgg cgatgctggg gaccgccgcc gccgtcgagg ccgcggtcgt tccctacccc
     2461 gagcacttcc gcggcctcgc cgcctcgctc gcgtcgcagg ccgcggtcgc gctcgagaac
     2521 agccggctgt accagtccat ccagaccctg ttcgagggct ttgtgcaggc ctcggtcacc
     2581 gccatcgagt cgcgggatcc cacgacctcc ggccactcct tccgggtggc cgacttcacg
     2641 gtggccctcg ccgccgcggt cgaccgcgcc gcccacggcc ccttccgcga cgtccggttc
     2701 tcggtggacg agatgaagga gatccgctac gcctcgctcc tccacgactt cggcaaggtg
     2761 ggcgtgcggg aggacgtact ggtcaaggcc aagaagctgc cgccgggcca gctcgagctg
     2821 atccgccagc gcggtgagat catccggcgc gggctcgagc tgcgccacgc gcggcgcaag
     2881 acggagtggc tcctccagca cggccgtgac gccttcgcgg accgctgcgc cgaatgggac
     2941 gcggagctgg ccgcggtcct cgccgacctc gacgtgcacc tgaaggcggt cgccgccgcg
     3001 aacgagccca ccgtcatgcc ggacgacgtc tcggccggga tccgtgacct ggccggacgg
     3061 gccttcctgg accacctcgg cgatccactc accctgatca cgccggagga ggcgcgcgtc
     3121 ctctcgatcc cccgcggcag cctgaccccg gacgagtacc gccagatcca gtcgcacgtc
     3181 gtccacacct accagttcct ccggcagatc ccgtggacga aggaactccg gcgcattccg
     3241 gagatcgccc gctcgcacca cgagaagctc aacgggtcgg gctaccccgc gggctggcga
     3301 gcgccggaga tcccggtcca gacgcggatg atgacgatct ccgacatctt cgacgcgctc
     3361 acctcgcgcg accggccgta caagcccgcg gtcccggtgg agcgcgcgct cgacatcctc
     3421 ggccaggagc ggcaggccgg agccctcgac ggcgagctgc tctccctctt catcgcggtg
     3481 cgcccctggg agcgggtcga tccggcctga tcccggttct tcgcgccctc aggccgcgag
     3541 cagggccgcc agcacgcccc acgagaccac gcccagcacc atcgcggccg ccc
//
LOCUS       DATMCV010001084         3102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_725397, whole genome shotgun sequence.
ACCESSION   DATMCV010001084 DATMCV010000000
VERSION     DATMCV010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725397"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VNU03_24800"
     CDS             <1..390
                     /locus_tag="VNU03_24800"
                     /inference="COORDINATES: protein motif:HMM:NF037735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HWP77766.1"
                     /translation="EVLNEIVASARADVVISSTWRHGRTIAELQAMLEAKGFTGRVVD
                     KTPTGAPGAERGDEIAAWLAEHAMGGGYVIIDDHVDMGELRTELVLTHPGRGLQPADA
                     PRAIEILMRAHGRPRRVAGSSDSATPR"
     gene            406..1635
                     /locus_tag="VNU03_24805"
     CDS             406..1635
                     /locus_tag="VNU03_24805"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77767.1"
                     /translation="MRRWLFTGWTVVAGAFFGYAIAQGLMHSYAVFLVAYLGEFGWGR
                     AETSVAYSISQLVIGISSPLVGVLVDRLGTRVLVTAGAILLAGGLAANAAVSSLWQVV
                     LLYGLVMTLGSNCLGLVVSVPILSRWFVRRRGLAISIVQSANGFGRAASAPMVQLLIS
                     SIGWRRSYLALAAMMAVAVVPIVRAFPTREPAAADAGPEDPARRAAPSVEAWTVAAAM
                     RTPHFWLLFAVYLLTGLGSFLVSLHQLAWAIDVGFDPLYAASVLGTGSLLAVAGTIGT
                     GTISDYIGREVSAILAYLVSIVGVICALFVTSPDQHALLWLHACFFGLTWGARGPQIT
                     AKTADLFQGPHLGAILGVISIGTGLGAGGGAWASGLIFDLSGSYRLAFQLSIASYAVG
                     CGAFWMLRRPARPRLGG"
     gene            complement(1624..2538)
                     /locus_tag="VNU03_24810"
     CDS             complement(1624..2538)
                     /locus_tag="VNU03_24810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWP77768.1"
                     /translation="MAFYAVPFRAGDRVITARAEYFSNYLAFLQMKARVGIEIDVVDS
                     DASGQSDLAALEHAIRPRTKLIAITHVPGRGGLVNPAAEVGRIAARHDLLYLLDACQS
                     VGQLPVDVRQIGCHMLSATGRKYLRGPRGTGFLYVRRDTIARLEPPFIDLHAATWTDA
                     DTYVIRDDARRFENWERFVAGQIGLGVAARYATQVGMDAIEARVKALAALLRGELAKH
                     PGVSVHDRGAELCGIVSFLKDGEAPARTRDRLRAMNINAHEAHLSRLDLPTGGARSLV
                     RASVHYYNDESEVERFVRAVASRSGLAS"
     gene            2642..>3102
                     /locus_tag="VNU03_24815"
     CDS             2642..>3102
                     /locus_tag="VNU03_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWP77769.1"
                     /translation="MLDVAQLRADTPGVDMVLHFNNAGSALPPRPVVDAVIGHLQREA
                     SIGGYEAAAEAADRIEDAYDAVATLIGAGPAEIAIVENATRAWDMAFYAVPFRAGDRV
                     ITARAEYLSNYLAFLQIKARAGIEIDVVDSDASGQIDLAALERAIRLRTKL"
BASE COUNT          406 a         1081 c         1115 g          500 t
ORIGIN      
        1 gaggtgttga acgagatcgt cgcgagcgct cgcgcggacg tcgtgatctc gtccacctgg
       61 cggcatggcc ggacgatcgc ggagctgcag gcgatgctgg aggcgaaggg gttcaccggg
      121 cgcgttgtcg acaagacccc gaccggggct cccggcgccg agcggggaga cgagatcgcg
      181 gcctggctcg cggagcacgc catgggcggt ggctatgtga tcatcgacga tcacgtcgac
      241 atgggagagc tccgcaccga gctcgtcctg acgcatccgg gccgagggct acaacccgcc
      301 gacgcaccgc gggccatcga gatactcatg cgagcccacg gccggccgcg aagggtggcc
      361 gggtcgtcgg actccgccac gcctcggtag agaagactgg cgcccgtgcg tcggtggctc
      421 ttcaccggct ggaccgtggt ggcgggcgcc ttcttcggct acgcgatcgc gcaggggctg
      481 atgcactcct acgcggtgtt cctcgtggcc tacctcgggg agttcggctg gggccgggcc
      541 gagacctcgg tggcctactc gatctcgcag ctcgtgatcg ggatcagctc gcccctcgtc
      601 ggtgtcctcg tggatcgcct cggcacccgc gtgctcgtca ccgcgggcgc gatcctgctg
      661 gcgggggggc tcgcggccaa cgcggcggtg tcctcgctct ggcaggtcgt cttgctctac
      721 ggcctcgtga tgacgctggg gtcgaactgc ctcggcctcg tggtctcggt gccgatcctc
      781 tctcgctggt tcgtgcggcg acgggggctc gccatctcga tcgtccagtc cgccaacggt
      841 ttcgggcggg ccgcgtccgc cccgatggtg cagctcctga tctcctcgat cggctggcgg
      901 cgctcgtacc tggccctcgc cgcgatgatg gcggtggcgg tggtcccgat cgtccgcgcc
      961 ttcccgactc gcgagcccgc cgcggcggac gcgggacccg aggatccggc acggcgggcc
     1021 gccccgtccg tcgaggcctg gacggtggcc gcggcgatgc ggacgccgca cttctggctg
     1081 ctcttcgcgg tgtacctgct caccggcctc gggagcttcc tggtctcgct gcaccagctc
     1141 gcctgggcga tcgacgtggg cttcgacccg ctctacgcgg ccagcgtgct cggcaccggg
     1201 agcctgctcg cggtggccgg gaccatcggc accgggacga tctcggacta catcgggcgc
     1261 gaggtctccg cgatcctcgc gtacctggtc tcgatcgtgg gcgtgatctg cgcgctcttc
     1321 gtcacgagcc ccgaccagca cgcgctgctg tggctgcacg cctgcttctt cgggctcacc
     1381 tggggcgcgc gcggcccgca gatcaccgcc aagaccgccg atctcttcca gggcccgcac
     1441 ctcggggcca tcctgggcgt gatctcgatc ggcaccggcc tcggggcggg gggcggggcc
     1501 tgggcgagcg ggctgatctt cgatctctcg ggcagctacc gcctcgcgtt ccagctgtcc
     1561 atcgcttcgt acgcggtcgg ctgcggggcc ttctggatgc tgcgccggcc cgcgcgaccg
     1621 cgcctaggag gctagaccgg acctcgaagc caccgcgcga acgaagcgct ccacctcgga
     1681 ctcatcgttg tagtagtgca cgctggcgcg gacgagcgag cgggctccgc cggtgggcag
     1741 atcgagccgc gacaggtggg cctcgtgggc gttgatgttc atggcgcgca gccgatcgcg
     1801 cgtccgggcc ggcgcttcgc cgtccttgag aaaggagacg atcccgcaca gctccgcccc
     1861 gcgatcgtgc acgctcaccc cggggtgctt cgcgagctcc ccccgcagca gggcggcgag
     1921 cgccttgacc cgcgcttcga tcgcgtccat ccccacctgc gtcgcgtagc gggcggcgac
     1981 gcccaggccg atctggccgg ccacgaatcg ctcccagttc tcgaatcggc gcgcgtcgtc
     2041 gcggatgacg taggtgtcgg cgtcggtcca cgtggcggcg tggaggtcga tgaacggggg
     2101 ctcgagcctc gcgatggtgt cacggcgcac gtagaggaag ccggtgccgc ggggcccgcg
     2161 cagatacttg cggccggtgg ccgagagcat gtggcagccg atctgccgca cgtcgaccgg
     2221 gagctggccg accgactggc aggcgtcgag cagatagagg aggtcgtggc gcgcggcgat
     2281 gcggccgacc tccgccgcgg gattgacgag cccgcctctc ccgggcacgt gggtgatcgc
     2341 gatcagcttc gtgcggggac ggatcgcgtg ctcgagcgcg gcgaggtcgc tctgaccgga
     2401 cgcgtcgctg tcgaccacgt cgatctcgat tcccacccgc gccttcatct ggaggaacgc
     2461 gagatagttc gagaagtact cggcgcgtgc ggtgatgacg cggtcgcccg cgcggaaggg
     2521 aactgcgtag aacgccatgt cccaggcgcg ggtggcattt ttccttcgag ggcacgcgcg
     2581 gggtcggcaa tcgcaccgac gacgtccgcc cgtgtagcct gaccttcgtc aagtaggagc
     2641 gatgctcgac gtcgcacagc tgagggcgga cacaccgggc gtcgacatgg tccttcactt
     2701 caacaatgcc ggctcggccc tcccgccgcg gccggtcgtc gacgccgtga tcggccacct
     2761 gcagcgcgag gcctccatcg gcggctacga ggcggcggcc gaagcggccg atcgcatcga
     2821 ggatgcctac gacgccgtcg ccacgctgat cggggcaggt ccggccgaga tcgcgatcgt
     2881 ggagaacgcc acgcgcgcct gggacatggc cttctacgcg gtccccttcc gggcgggcga
     2941 ccgcgtcatc accgcgcggg ccgaatacct gtcgaactac ctcgccttcc tgcagatcaa
     3001 ggcgcgcgcg gggatcgaga tcgacgtggt cgacagcgac gcatccgggc agatcgacct
     3061 cgccgcgctc gagcgcgcga tccgtctccg cacgaagctg at
//
LOCUS       DATMCV010001085         3041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_194688, whole genome shotgun sequence.
ACCESSION   DATMCV010001085 DATMCV010000000
VERSION     DATMCV010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194688"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VNU03_24820"
     CDS             <1..679
                     /locus_tag="VNU03_24820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HWP77770.1"
                     /translation="WTLMTLVPVPGVGRGVLEPEKNLSNWLDFRLLGVNHVWHETRTW
                     DPEGLLSTLGAVASVLCGVLAGHWIRSRHRMDAKAFGLFYAGGMVLLLGWVWSHIFPI
                     NKSLWTSSYVLLSAGLACLVLAAIIWLVDIEGYRGWTAPFLVFGTNAITAYWLSTLCG
                     IVLDWITLGGPAEADQVVLKTYLYENFFASWLAPANASLAYAVAFVVVWLGLLSFLYR
                     RRIFIRI"
     gene            complement(691..1479)
                     /locus_tag="VNU03_24825"
     CDS             complement(691..1479)
                     /locus_tag="VNU03_24825"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWP77771.1"
                     /translation="MDSTLSRRGFIASGLGAAAGALLAPRRSAGAASERFLIVNADDL
                     GLSAEVDRGLFEAHDAGIVTSASLLVDGPNAEAAIQQARQRPKLGIGIHVSFDDRGKL
                     FTDMQDLAAVQQQIDRQLAAFIRMVGSPPDHIDSHHHMHRLFNVARLFLVAGRRYNVP
                     VRGFSDVVFVGRFYGQPEFGKADLSKISVEVLVAMLRAVKPGATEISCHPGYAETRPD
                     AIYDREREVELRTLTDEQVKKTISEEGIRLISYRDYNRMSRDAR"
     gene            1557..2132
                     /locus_tag="VNU03_24830"
     CDS             1557..2132
                     /locus_tag="VNU03_24830"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWP77772.1"
                     /translation="MRLVPVALALVLAVILSLGLSLGATSVRAQAPRVYEPEQSRPWA
                     VYFSPGGGAAQAVVEAIGRAGHTVLVQAASLSSPQITKSLADAQHRGVKVEVILDRKH
                     AKGRPSSAESLMRDGVVVLIDRAHPANSNVMVIDAEVVLTGSFGFTVAADRESVENLL
                     IIHAPTLAARYGENWQAHAAHSVRYSASATP"
     gene            complement(2267..>3041)
                     /locus_tag="VNU03_24835"
     CDS             complement(2267..>3041)
                     /locus_tag="VNU03_24835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77773.1"
                     /translation="KFQVLQSNAPHIWYWHLNMKDEKFKDVRVRKALQMSVDKDRMCK
                     ELLRGTAKPAWSMISPATVAHDPKYKPYSYDPEKAKALLKDAGLGSGFETTFWTSTSG
                     SGQMIPVAMAEWIQRDLAKVGIKVKLETFDWITYLSKMFQGLRPGHGAYQLSWGMTSN
                     FWIDIVARSTRQPDKGVNVGWYANPKVDGLLDTARGELDEKKRAAVYKQIDRIIMEED
                     ASFFPICNDLNLVVLSPKVKGFVNPPEEWFQLWTPWVEA"
BASE COUNT          432 a         1020 c         1039 g          550 t
ORIGIN      
        1 ctggacgctc atgacgctgg tgcccgtccc cggggtcggg cgcggcgtgc tcgagccgga
       61 gaagaacctc tcgaactggc tcgacttccg cctgctcggg gtcaaccatg tctggcacga
      121 gacgcggacc tgggacccgg aggggttgct gagcacgctg ggtgcggtcg cctcggtgct
      181 ctgcggggtg ctggccggcc actggatccg ctcccgccac cggatggacg cgaaggcctt
      241 cgggctcttc tacgcgggcg gcatggttct cctgctcggg tgggtctgga gccacatctt
      301 cccgatcaac aagagcctgt ggaccagctc ctacgtgctg ctctcggcgg gcctcgcctg
      361 tctggtgctc gcggcgatca tctggctggt cgacatcgag ggctaccgcg gctggacggc
      421 cccgttcctg gtcttcggga ccaacgcgat caccgcctac tggctgtcga ccctgtgcgg
      481 gatcgtgctc gactggatca cgctcggggg gccggcggag gccgaccagg tggtgctcaa
      541 gacctacctc tacgagaact tcttcgcctc gtggctcgcg cccgcgaacg cgtcgctcgc
      601 ctacgccgtc gccttcgtgg tggtgtggct ggggctgctg agcttcctct accggcgacg
      661 catcttcatc cggatatgac ccgggcggac ctagcgcgcg tcccgggaca tccggttgta
      721 gtcgcggtag ctgatcaggc ggatcccttc ctcgctgatc gtcttcttga cctgctcgtc
      781 ggtcagcgtc cgcagctcca cctcccgctc gcgatcgtag atcgcgtcgg ggcgggtctc
      841 ggcgtagccg ggatggcagg agatctcggt ggcgccgggc ttcaccgccc ggagcatcgc
      901 caccaggacc tccacgctga tcttggacag gtcggccttg ccgaactcgg gctggccgta
      961 gaagcggccc acgaagacca cgtccgagaa gccgcgcacc gggacgttgt agcgccggcc
     1021 cgcgacgagg aagaggcgcg ccacgttgaa gaggcggtgc atgtggtggt gcgagtcgat
     1081 gtggtcaggc ggactgccca ccatccggat gaacgcggcg agctgccggt cgatctgctg
     1141 ctggaccgcg gcgagatcct gcatgtcggt gaacagcttc ccccggtcgt cgaaggagac
     1201 gtggatgccg atcccgagct tcgggcgctg ccgcgcctgc tggatcgcgg cctccgcgtt
     1261 cgggccgtcc accaggaggc tggcgctggt cacgatgccc gcgtcgtggg cctcgaagag
     1321 gccgcggtcg acctcggcgg acaggccgag atcgtctgcg ttgacgatga ggaatcgttc
     1381 ggaggccgcg cccgccgatc ggcgcggtgc gaggagggcg ccggcggcgg ctccgaggcc
     1441 ggaagcgatg aagccgcgtc tcgacagggt cgagtccatg gttctttcaa gatagcccga
     1501 ccgggtaaaa aatgccctgg atagtgtcgg ttgccagtcg tgcgatgatc tcccctatga
     1561 ggctcgtccc ggtcgccttg gccctcgtcc tggccgttat tctgagcctt gggctgagcc
     1621 tcggggcgac gagcgtccgg gcccaggctc cgcgggtcta cgagcccgag cagtcccgcc
     1681 cctgggcggt ctacttctcg ccgggtggcg gcgcggccca ggcggtggtg gaggcgatcg
     1741 ggcgggccgg ccacaccgtg ctcgtgcagg ccgcctcgct cagctcgccc cagatcacca
     1801 agtcgctcgc ggacgcccag caccgcggcg tcaaggtcga ggtcatcctc gaccgcaagc
     1861 acgccaaggg gcgcccctca tccgctgagt cgctcatgcg ggacggggtc gtggtgctga
     1921 tcgaccgcgc gcatcccgcc aacagcaacg tgatggtgat cgacgcggag gtcgtgctga
     1981 ccgggagctt cggcttcacg gtcgcggcgg accgcgagag cgtggagaat ctcctgatca
     2041 tccacgcgcc cacgttggcc gcgcgctacg gcgagaactg gcaggcccac gcggcccact
     2101 ccgtccgcta ctccgcctcc gctaccccct gacccgcggg ctgctgaaaa aggcccatct
     2161 gcttcgttgg cgccggtggc tgcacgctca acgtacggag agtacgcctc gcgtgcagcc
     2221 agccggcgcc gcctcgcatc tggacctttt tgagcagccc gctaggttat gcctccaccc
     2281 agggggtcca gagctggaac cactcctccg gagggttgac gaagcccttc accttcgggc
     2341 tcagcaccac caggttgagg tcgttgcaga tggggaagaa cgaggcgtcc tcctccatga
     2401 tgatccggtc gatctgcttg tagaccgccg cgcgcttctt ctcgtcgagc tcaccgcggg
     2461 cggtgtcgag gaggccgtcg accttcgggt tcgcgtacca gcccacgttg acgcccttgt
     2521 cgggctgccg cgtggagcgg gccacgatgt cgatccagaa gttgctggtc atgccccagg
     2581 agagctggta ggcgccgtgg cccggccgta gcccctggaa catcttcgag agataggtga
     2641 tccagtcgaa ggtctcgagc ttgaccttga tgcccacctt ggcgaggtcg cgctggatcc
     2701 actccgccat cgccaccggg atcatctgcc ccgagccgga ggtcgacgtc cagaacgtgg
     2761 tctcgaagcc cgagccgagg cccgcgtcct tgagcagcgc cttggccttc tcggggtcgt
     2821 aggagtaggg cttgtacttc gggtcgtggg cgacggtggc gggggagatc atgctccacg
     2881 ccggcttcgc ggtgccgcgc agcagctcct tgcacatccg gtccttgtcg accgacatct
     2941 ggagcgcctt gcgtacccgc acgtccttga acttctcgtc cttcatgttc aggtgccagt
     3001 accagatgtg cggcgcgttc gactgcagca cctggaactt c
//
LOCUS       DATMCV010001086         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_793468, whole genome shotgun sequence.
ACCESSION   DATMCV010001086 DATMCV010000000
VERSION     DATMCV010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793468"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VNU03_24840"
     CDS             complement(<1..412)
                     /locus_tag="VNU03_24840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77774.1"
                     /translation="MIRAEATRRQWRWVGVATLAMLSLVRPAAAFDPEATFARGTTIF
                     GLQVGGGAANNIEGHHYVSDISFITEIPRLSYLFFDPFGSGLLRSAFEPGLEGWFQQY
                     LSPHSATAQGLKVTGRYHLIGLGRFVPYLEGTAGA"
     gene            complement(409..1440)
                     /locus_tag="VNU03_24845"
     CDS             complement(409..1440)
                     /locus_tag="VNU03_24845"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWP77775.1"
                     /translation="MRAQVFHGPGDLRLEEIPVPEPDAGGLVVRVQAALTCGTDVKTL
                     RRGHPVMIPHVPTVFGHELAGVVTAVGRGVTGIREGDRVVAANSAPCGHCRLCGAGRP
                     NLCENLLFVNGAYGEHIALPPRLVARNTVPIAAGLAASSAAFAEPLACALLGIERGRV
                     EPGMTVVIFGHGPLGCLLALVARERGARVVLVGRAGWRLDRVRAMGLGECVDARDVLD
                     MGAHLRGLTGGRGADVTVDATGRPEVWEQAIDGVGRGGTVVLFGGCAPGTSISVDTRR
                     AHYEELTLVGAFHHTPALIRRAVELLESRAIDPGPLVTHRMGLAQVKEALAMMERGET
                     MKVLVDPER"
     gene            complement(1442..>2086)
                     /locus_tag="VNU03_24850"
     CDS             complement(1442..>2086)
                     /locus_tag="VNU03_24850"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP77776.1"
                     /translation="NVEHAAFRLPAAMADETASFTEPLACCLRAVKRARIAPGDTALV
                     VGLGSIGCLLVQLLTLAGASAVLADDVLPARRALGRRMGALVPDDPAAVDAAVAQVSD
                     GRGVDCVVITAGGAAVLPAAAARVRDGGALHYFAGGAGESLPLSLAALYHRELTLSAT
                     YSSSPAELAEAFELLVTGRVRVDGLISHRLPLGRLGEGVELMRRHEAVKVYVTP"
BASE COUNT          309 a          804 c          721 g          252 t
ORIGIN      
        1 cggcacccgc ggtgccctcg agatacggga cgaagcggcc gagcccgatc aaatggtagc
       61 ggccggtcac cttgagcccc tgggcggtgg ccgagtgcgg cgagaggtac tgctggaacc
      121 acccctcgag gccgggctcg aacgcactcc ggagcagccc cgagccgaag gggtcgaaga
      181 acagatacga cagccgcggg atctcggtga tgaatgagat atcgctcacg tagtggtggc
      241 cctcgatgtt gttcgcggcg ccaccaccaa cctgcagtcc gaagatcgtc gtgccccggg
      301 cgaaggtcgc ctccgggtcg aatgcggccg ccggccgtac cagcgacagc atcgccagcg
      361 tggccacccc tacccaccgc cactgccttc gcgtcgcctc ggctcggatc atcgctctgg
      421 gtccaccagc accttcatgg tctcaccgcg ctccatcatc gccagcgctt ctttcacctg
      481 cgccagtccc atgcggtggg tcacgagcgg tcccggatct atcgccctgg attcgagtag
      541 ttccaccgca cggcgaatca gggccggcgt gtggtggaag gcccccacca gcgtgagctc
      601 ttcgtagtgc gcccggcggg tgtccacact gatggacgtg cccggggcgc aacccccaaa
      661 caataccacg gtgcctcccc gccccacccc atctatggcc tgctcccaga cctctggacg
      721 cccggtcgca tccaccgtca cgtcggcccc ccggcccccg gtcagccccc ggaggtgtgc
      781 gcccatgtct aggacatcgc gggcgtccac gcactcgccg agtcccatgg cccgcacccg
      841 gtcgagccgc cagcccgccc tgcccacgag caccacccga gccccgcgct cgcgggccac
      901 gagagccagc aggcacccga gcgggccgtg gccgaagatc accaccgtca tgcccggctc
      961 gacccggccc cgctcgatcc cgagcagggc gcaggccagc ggctcggcga aggccgcgct
     1021 cgacgcggcc aggccggcgg cgatcgggac cgtgttgcgg gcaaccagcc gcgggggcag
     1081 cgcgatgtgc tcgccgtagg cgccgttgac gaagagcagg ttctcgcaca ggttgggccg
     1141 gcccgccccg cagagccggc agtgcccgca cggggccgag ttggccgcga cgacccggtc
     1201 gccctcccgg atgcccgtca cgccgcggcc gaccgcggtc accacgccgg ccagctcgtg
     1261 accgaagacg gtgggcacgt gggggatcat caccgggtgg ccgcgccgca gggtcttcac
     1321 gtcggtgccg caggtgagcg cggcctgcac ccggaccacg aggccgcctg catcgggctc
     1381 gggcaccggg atctcctcga gccggaggtc gccgggtccg tggaagacct gcgcgcgcat
     1441 gctagggggt gacgtagacc ttcaccgcct cgtgccgccg catcagctcg accccttccc
     1501 cgagccggcc gagcggcagc cggtggctga tcagcccgtc gacgcgcacg cggcccgtga
     1561 ccagcagctc gaacgcctcg gcgagctcgg ccggcgacga cgagtaggtc gcgctcaggg
     1621 tcagctcgcg atggtagagc gcggcgagcg acaggggcag gctctcgccc gcgccacccg
     1681 cgaagtagtg cagcgcccct ccgtcgcgca cgcgcgcggc ggccgcgggc aggaccgccg
     1741 cgccgcccgc ggtgatcacg acgcaatcga cgccgcgccc atcgctgacc tgggccaccg
     1801 ccgcgtcgac ggcggccggg tcgtcgggca ccagcgcgcc catgcgacgg ccgagcgccc
     1861 gccgcgcggg cagcacatcg tcggccagga ccgccgaggc gccggccagg gtcaggagct
     1921 ggacgagcag gcagccgatc gatcccaggc ccaccacgag cgcggtgtcc cccggcgcga
     1981 tccgggcgcg cttgaccgcg cgcaggcagc acgcgagcgg ctcggtgaac gacgcggtct
     2041 cgtccgccat cgccgcgggc aggcggaagg cggcgtgctc gacgtt
//
LOCUS       DATMCV010001087         3118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_725706, whole genome shotgun sequence.
ACCESSION   DATMCV010001087 DATMCV010000000
VERSION     DATMCV010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725706"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VNU03_24855"
     CDS             <1..282
                     /locus_tag="VNU03_24855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HWP77777.1"
                     /translation="LGEVLELSDRPLERFRFSDRLWTRVVYDFVLAYRTRVVYRSHAA
                     QSLAPLYLGRAASVVLETQGRPPLAMLQSAARLGGMFEEEKPYLVDRWR"
     gene            279..2261
                     /locus_tag="VNU03_24860"
     CDS             279..2261
                     /locus_tag="VNU03_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HWP77778.1"
                     /translation="MKPARRPIVIEHVRPAVDDGRYPAKRVVGDVLTVSADIFKEGHD
                     LLAARICYRTHDESAWREAPLRPVDNDGWGGAFRLEANTRYRYTVEAWTDTFGSWVEE
                     MRRRIAGGQTDLASELLEGAEMVRRAQAVAAGPDGAALGRTLERLAGAPSQDARLDVL
                     LDGELRQLMARVAPRADLTRHDRELEVVVDRPEAAFAAWYEMFPRSQGRVPGRHGTFD
                     DCIERLPDIRRMGFDVVYLPPIHPIGRTARKGPDNALAAGPGDPGSPWAIGSPEGGHT
                     AVHPDLGTLEGFRRLVKSAQGLGLEIALDFAIQCSPDHPWVREHPEWFYPRPDGTIKY
                     AENPPKKYQDIYPINFATEAWASLWDALLGVVRFWIEQGVRTFRVDNPHTKPLDFWAW
                     LIRDVQERDPDVIFLSEAFTRPKVMRALGKAGFTQSYTYFTWRNFKEELTEYLEELTR
                     SEMAEYFRGNFFVNTPDILPEVLQRGGPPAFRMRATLAATLASVWGVYSGFELGEAAA
                     LPGSEEYQASEKYEIRVRDWDAPGNLKDHIARLNTIRRENRALHSMSELRFYASSSPH
                     VLFYGKMTPARDNVILVAVNLDPFATHEATLELPLEEIGIAADETYELHELLADQRRL
                     IRGARHTVSLDPRVATAHVYRVGRWRRRERDFDYYA"
     gene            2269..>3118
                     /locus_tag="VNU03_24865"
     CDS             2269..>3118
                     /locus_tag="VNU03_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWP77779.1"
                     /translation="MSDRDPLWYKDAVFYELHVRAFRDGNDDGIGDFIGLTSQLGHLK
                     ELGVDCLWLLPFFRSPLRDDGYDVADYRAIHPDYGTMADFDRFLEAAHGHGMRVISDL
                     VVNHTSDQHPWFQAARRDSASPYRDYYVWSDTDERYRDARIIFVDTEQSNWTWDPVAK
                     AYYWHRFFHHQPDLNFDNPQVRRELLDVMRFWLDKGLDGFRCDAVPYLFEREGTSCEN
                     LPETHEFLREIRRTIDAQYQGRILLAEANQWPADVRPYFGDGDEFHMAFHFPLMPRIF
                     LAVRHGD"
BASE COUNT          464 a         1174 c         1029 g          451 t
ORIGIN      
        1 ctgggcgagg tgctcgagct gagcgatcgg cctctggagc gcttccgctt ctcggatcgg
       61 ctgtggactc gggtggtcta cgacttcgtc ctggcctacc gcacgcgcgt cgtctaccgg
      121 agccacgccg cgcagtcgct ggccccgctc tacctcggcc gcgccgcgtc ggtggttctg
      181 gagacgcagg gccgcccccc gctcgccatg ctgcagagcg cggcgcgcct gggcggcatg
      241 ttcgaggagg agaagcccta cctcgtggac cgctggcgat gaagccggcg cgccgcccca
      301 tcgtcatcga gcacgtgcgt cccgcggtgg acgacggccg gtacccggcc aagcgcgtcg
      361 tgggcgacgt cctcaccgtc tccgcggaca tcttcaagga gggccacgat ctgctggccg
      421 cccggatctg ctaccgcacc cacgacgagt cggcgtggcg ggaggcaccc ctgcggccgg
      481 tcgacaacga cggctggggc ggcgcgttcc gcctcgaggc caacacccgc taccgctaca
      541 ccgtggaggc ctggaccgac accttcggct cgtgggtgga ggagatgcgg cggcgcatcg
      601 cgggcgggca gaccgacctc gcgagcgagc tgctcgaggg cgcggagatg gtccgccgcg
      661 cgcaggccgt cgcggccggg cccgacgggg ccgcgctcgg ccgcacgctc gagcggctcg
      721 cgggcgcgcc ctcccaggac gcgcgcctcg acgtcctgct cgacggcgag ctccgccagc
      781 tcatggcccg ggtggcgccg cgcgccgacc tcacccgcca cgaccgcgag ctggaggtgg
      841 tggtggaccg cccggaggcc gccttcgcgg cgtggtacga gatgttcccg cgctcgcagg
      901 gccgggtgcc cgggcgccac ggcaccttcg acgattgcat cgagcggctt cccgacatcc
      961 gccggatggg attcgacgtc gtctacctgc cgcccatcca cccgatcggc cgcaccgccc
     1021 gcaagggccc ggacaacgcg ctcgccgccg ggcccggcga cccgggcagc ccgtgggcga
     1081 tcggctcgcc ggagggcggc cacaccgcgg tgcaccccga cctcggcacg ctcgagggct
     1141 tccgccggct cgtgaagagc gcgcagggcc tggggctgga gatcgccctc gacttcgcga
     1201 tccagtgctc gcccgatcac ccgtgggtgc gcgagcaccc cgagtggttc tacccgcggc
     1261 cggacgggac gatcaagtac gcggagaacc cgcccaagaa gtaccaggac atctacccga
     1321 tcaacttcgc gacggaggcg tgggcctccc tctgggacgc gctgctcggg gtggtccgct
     1381 tctggatcga gcagggggtg cggaccttcc gggtcgacaa cccccacacc aagccgctcg
     1441 acttctgggc gtggctcatc cgggacgtac aggagcgcga tcccgacgtg atcttcctct
     1501 ccgaggcctt cacgcgcccc aaggtcatgc gggcgctcgg caaggccggc ttcacccagt
     1561 cctacacgta cttcacgtgg cgcaacttca aggaggagct gaccgagtac ctggaggagc
     1621 tgacccgctc ggagatggcc gagtacttcc gcggcaactt cttcgtcaac accccggaca
     1681 tcctccccga ggtgctccag cgcggaggcc cgcccgcctt ccgcatgcgg gcgacgctgg
     1741 ccgcgacgct ggcctcggtg tggggcgtct acagcggctt cgagctcggg gaggccgcgg
     1801 ccctgccggg cagcgaggag taccaggcct ccgagaagta cgagatccgg gtccgcgact
     1861 gggacgcccc cggcaacctc aaggaccaca tcgcgcgcct caacaccatc cgccgcgaga
     1921 accgggcgct gcactcgatg agcgagctgc gcttctacgc ttccagcagc ccgcacgtgc
     1981 tcttctacgg caagatgacc cccgcccgcg acaacgtgat cctggtcgcg gtgaacctcg
     2041 acccgttcgc cacccacgag gccaccctcg agctcccgct cgaggagatc ggcatcgcgg
     2101 ccgacgagac ctacgagctg cacgagctgc tcgccgacca gcggcgcctg atccgcggcg
     2161 cccgccacac cgtgagcctc gatccgcggg tggccaccgc ccacgtctat cgcgtcggcc
     2221 gctggcgccg gcgcgagcgt gacttcgatt attacgcgtg aggcctgagt gagcgatcgc
     2281 gatccgctct ggtacaagga cgcggtgttc tacgagctgc acgtccgcgc gttccgggat
     2341 ggcaacgacg acggcatcgg cgacttcatc gggctcacca gccagctcgg gcatctcaag
     2401 gagctggggg tcgactgcct ctggctgctg ccgttcttcc gctcgccgct gcgcgacgac
     2461 ggctacgacg tggccgacta ccgggccatc caccccgact acggcacgat ggccgacttc
     2521 gaccgcttcc tcgaggcggc ccacggccac ggcatgcggg tgatctccga tctcgtggtc
     2581 aaccacacct cggaccagca cccgtggttc caggcggccc ggcgagattc cgcctccccc
     2641 taccgcgact actacgtctg gagcgacacc gacgagcgct atcgcgacgc ccggatcatc
     2701 ttcgtcgaca ccgagcagtc caactggacg tgggacccgg tggcgaaggc ctactactgg
     2761 caccgcttct tccaccacca gccggacctc aacttcgaca atccccaggt gaggcgcgag
     2821 ctgctggacg tcatgcggtt ctggctcgac aaggggctcg acggcttccg ctgcgacgcg
     2881 gtgccctacc tcttcgagcg cgagggcacc agctgcgaga acctcccgga gacgcacgag
     2941 ttcctccgcg agatccgccg caccatcgac gcccagtacc aggggcggat cctcctagcc
     3001 gaggccaacc agtggcccgc cgacgtccgc ccctacttcg gcgacggcga cgagttccac
     3061 atggccttcc acttcccgct catgccgcgc atcttcctgg ccgtccgcca cggcgacc
//
LOCUS       DATMCV010001088         3381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_194995, whole genome shotgun sequence.
ACCESSION   DATMCV010001088 DATMCV010000000
VERSION     DATMCV010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194995"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2526)
                     /locus_tag="VNU03_24870"
     CDS             complement(<1..2526)
                     /locus_tag="VNU03_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWP77780.1"
                     /translation="MNPSRIFIERPVATSLLMVAVMLVGIVAYQFLPLAALPEVDYPT
                     IQVQTLYPGASPEVMTSAVTAPLERQFGQMPSLKQMSSTSSAGASVITLQFALNLSLD
                     VAEQEVQAAINAAGNLLPSDLPAPPIYAKINPADVPVLTLALTSKSLSLPQIEDVAES
                     RLAQKVSQLPGVGLVRVVGGQRPAVRVQAHLRALAAYGLNIDDLRTTINNANVNLPKG
                     NFDGPARAYAVNANDQLQSADAYRSIVVAYRNGAPVRLSDVADVLDGAENPKLAAWMN
                     RTPAVVVNIQRQPGANVIEVVDRVKALLPQLESSLPAAIQVTVLTDRTNTIRASVRDV
                     NFELVLAVVLVVLVIFLFLGSLRATVIPSLAVPLSLVGTFGVMYLAGFSLNNLSLMAL
                     TIATGFVVDDAIVMIENITRYIEQGERPLAAALKGSAQIGFTIVSLTISLIAVLIPLL
                     FMGDVVGRLFHEFAITLAVTILISAGVSLSLAPMMCAKILRPERAEDQGRIARFTTRS
                     FDRLIRYYDRSLGWILEHQPATLLVAVATLALTVFLYVVIPKGFFPVQDTGLIQGISE
                     ASQAVSFGRMAELQQALAARILRDPDVVSLSSFIGVDGTNPTLNTGRFLINLKPREDR
                     TATASDVIRRLRGDVAAVGGISLFMQPVQDLSIDTTVSRTQYQFVLVDANPDEFTVWI
                     PRLIDRMNALPHVEDVASDLQNQGLSVYVEVDRATAGRFGITPATVDNALYDSFGQRI
                     VSTVFTQSNQYRVILEADPSLQESLQSLGSIYLPSSVAAGGQVPLSAIASVREESAPL
                     AVNHLGQFPAATVSFNLAPGASLGAAVDAITTAGREIGLTASMA"
     gene            complement(2523..>3381)
                     /locus_tag="VNU03_24875"
     CDS             complement(2523..>3381)
                     /locus_tag="VNU03_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWP77781.1"
                     /translation="LLHDAQIDLTRYRTLAEQDSIPQQQADTQAYLVRQYEGTVRSDQ
                     AMVDNARLNLEYCHIVAPVSGRVGLRQVDQGNYVQTSDPNGIVVITQLQPISVIFTLP
                     EDNVPAVMSRLAAATLPVTAYDRALITKLAEGKLRTVDNQIDTTTGMVRLRAEFDNRD
                     NRLFPNQFVTTLLLVDTLKGATLVPSAAVQRGAPGTFVYVVDGERVVHLQPVKLGPTD
                     GERVAVLSGATVGAQVVVDGADKLRDGAKVTLASDAGRAGGAPDRAPPASGPSSDSAQ
                     RGRRKGGTP"
BASE COUNT          509 a         1096 c         1280 g          496 t
ORIGIN      
        1 cgccatggac gcggtgagcc cgatctcgcg gccggccgtg gtgatcgcgt ccaccgccgc
       61 gcccagggag gcgccggggg ccaggttgaa ggacacggtg gcggcgggga actgcccgag
      121 gtggttgacc gcgagcggcg ccgactcctc gcgcaccgac gcgatggccg agagcggcac
      181 ctgcccgccg gcggccaccg aggaaggcag gtagatggag ccgagcgact gcagcgactc
      241 ctggagcgag gggtccgcct ccaggatgac gcggtactgg ttcgactggg tgaagacggt
      301 ggagacgatg cgctggccga aggagtcgta gagcgcgttg tcgaccgtgg ccggcgtgat
      361 gccgaagcgg cccgcggtgg cgcggtcgac ctccacgtag accgagagcc cctggttctg
      421 gaggtcgctc gccacgtcct ccacgtgcgg cagcgcgttc atgcggtcga tgaggcgggg
      481 gatccacacc gtgaactcgt cggggttggc gtccacgagc acgaactggt actgggtgcg
      541 gctcacggtg gtgtcgatgc tgaggtcctg gaccggctgc atgaagagcg agatcccgcc
      601 caccgcggcc acgtcccccc gcagtcgccg gatcacgtcg ctcgcggtgg cggtgcggtc
      661 ctcgcgcggc ttcaggttga tcaggaagcg cccggtgttg agtgtcgggt tcgtcccgtc
      721 caccccgatg aacgaggaga ggctcaccac gtcggggtcg cgcaggatgc gcgccgcgag
      781 cgcctgctgc agctcggcca tgcgcccgaa cgagaccgcc tgtgaggcct ccgagatgcc
      841 ctggatcagc ccggtgtcct gcaccgggaa gaagcccttg gggatgacca cgtagaggaa
      901 cacggtcagg gcgagcgtcg cgaccgccac caggagggtg gcgggctggt gctcgaggat
      961 ccacccgagc gagcggtcgt agtagcggat gagccggtcg aaggaccggg tggtgaagcg
     1021 cgcgatgcgg ccctggtcct ccgcccgctc ggggcgcagg atcttggcgc acatcatggg
     1081 cgcgagcgag agcgagaccc ccgccgagat caggatcgtc accgcgaggg tgatcgcgaa
     1141 ctcgtggaag agccgcccga ccacgtcgcc catgaagagc agcgggatca gcaccgcgat
     1201 cagcgagatg gtgagggaga cgatggtgaa cccgatctgg gccgacccct tcagggcggc
     1261 ggcgagcggg cgctcgccct gctcgatgta gcgggtgatg ttctcgatca tcacgatcgc
     1321 gtcgtcgacc acgaagccgg tcgcgatggt gagcgccatc agcgagaggt tgttcaggct
     1381 gaagccggcc aggtacatca ccccgaaggt gccgacgagg gagagcggca ccgccaggct
     1441 cgggatgacg gtggcccgca gactgcccag gaacaggaag atcaccagca ccaccagcac
     1501 caccgcgagc accagctcga agttcacgtc gcgcaccgac gcgcggatgg tgttggtgcg
     1561 atcggtcagc accgtcacct ggatcgcggc gggcagcgag ctctcgagct ggggcaggag
     1621 cgccttcacc cggtccacca cctcgatgac gttggcgccc ggctggcgct ggatgttgac
     1681 caccaccgcg ggggtccggt tcatccaggc cgcgagcttg gggttctcgg cgccgtccag
     1741 cacgtcggcc acgtcggaga ggcgcaccgg cgccccgttg cgataggcca ctacgatcga
     1801 gcggtaggcg tccgcgctct ggagctggtc gttggcgttg accgcgtagg cgcgcgcggg
     1861 cccgtcgaag ttgcccttgg gcaggttcac gttggcgttg ttgatggtgg tgcgcaggtc
     1921 gtcgatgttg agcccgtagg cggcgagggc gcgcaggtgg gcctgcaccc gcaccgcggg
     1981 ccgctggccg cccaccaccc gcaccagacc caccccggga agctgcgaga ccttctgggc
     2041 caggcggctc tccgccacgt cctcgatctg gggcagggag agcgacttgg aggtgagcgc
     2101 cagcgtgagc accggcacgt cggccgggtt gatcttggcg tagatcggcg gcgcgggcag
     2161 gtccgagggg agcaggttgc ccgccgcgtt gatcgcggcc tgcacctcct gctcggccac
     2221 gtcgaggctc aggttcagcg cgaactgcag cgtgatgacc gaggcccccg ccgagctggt
     2281 cgaggacatc tgcttgaggc tcggcatctg gccgaactgc cgctcgagcg gcgcggtcac
     2341 cgcggaggtc atcacctcgg ggctggcgcc cgggtagagg gtctggacct ggatggtcgg
     2401 gtagtccacc tcggggagcg cggcgagcgg caggaactgg taggccacga ttcccacgag
     2461 catgaccgcg accatcagca gcgaggtcgc gaccgggcgc tcgatgaaga tccgcgatgg
     2521 gttcacggcg tcccgccctt ccggcgaccc cgctgcgcgc tgtcggagga ggggcccgag
     2581 gcgggcgggg cgcggtcggg cgcgccgccc gcccgccccg cgtcgctcgc gagggtgacc
     2641 ttggccccgt cgcgcagctt gtcggccccg tcgaccacca cctgcgcccc caccgtggcc
     2701 ccggagagga ccgcgacccg ctcgccgtcg gtgggcccga gcttcaccgg ctgcaggtgg
     2761 acgacgcgct caccgtcgac cacgtagacg aacgtgccgg gcgcgccccg ctgcaccgcc
     2821 gcgctcggca cgagggtggc gcccttcagg gtgtcgacca gcagcagggt ggtcacgaac
     2881 tggttcggaa agaggcggtt gtcgcgattg tcgaactccg cgcgcaggcg caccatgccg
     2941 gtggtggtgt cgatctggtt gtcgacggtg cgaagcttgc cctccgcgag cttggtgatg
     3001 agcgcgcgat cgtaggcggt caccgggagg gtcgcggcgg cgagccggct catcaccgcg
     3061 ggcacgttgt cctcgggcag ggtgaagatc accgagatcg gctggagctg ggtgatgacc
     3121 acgatgccgt tcgggtcgct ggtctggacg tagttgccct gatccacctg gcgcaggccc
     3181 acgcgacccg agaccggcgc cacgatgtgg cagtactcga ggttgagacg cgcgttgtcg
     3241 accatcgcct ggtcggacct cacggtgccc tcgtactgcc ggacgaggta ggcctgggtg
     3301 tcggcctgct gctgggggat cgagtcctgc tcggcgagcg tgcggtagcg ggtgaggtcg
     3361 atctgggcgt cgtgcagcag g
//
LOCUS       DATMCV010001089         3348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_860062, whole genome shotgun sequence.
ACCESSION   DATMCV010001089 DATMCV010000000
VERSION     DATMCV010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860062"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VNU03_24880"
     CDS             complement(<1..898)
                     /locus_tag="VNU03_24880"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWP77782.1"
                     /translation="MAGSFVTDAELKTLLVDQLELVETAEFEKAQQMATRLKIPLERA
                     LAERGRIPISFLLAQLAQAWDVGFIELKVGDVQLDCLSSLREEYARSHGVMPFERQER
                     RLKVAMANPRDRLIVREMGQMTKLEIVPYLAPEGAIRRAQLLYKGNLREMLERSALRA
                     STEINRPARAAGPEAPGPQWHGAAAADPAARRGAPAEASAADLVTRILEYAVVTRASD
                     IHIEPYELETLVRYRVDGVLHEVLSLAPATHPSLVARIKILGAMRIDEHRIAQDGRFE
                     ADLSGFVVDLRVSVVPTQWGEKV"
     gene            complement(898..1284)
                     /locus_tag="VNU03_24885"
     CDS             complement(898..1284)
                     /locus_tag="VNU03_24885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77783.1"
                     /translation="MGETARRVLLAEDDRFLRKAAETALKRQGFTVLPAVDGEEALRT
                     ARAEAPHLILLDLIMPKMQGFEVLRALKADPATAAIPVIILSNLGQDSDVKQAMEAGA
                     AGYFVKANLSLQDLVKRVGELIGKGA"
     gene            complement(1291..1668)
                     /locus_tag="VNU03_24890"
     CDS             complement(1291..1668)
                     /locus_tag="VNU03_24890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77784.1"
                     /translation="MTTGGRILLVEDDRFLRRAAEASLRQRGFAVTVAADGEEALVKV
                     RTEIPDLILLDLLMPKVTGIEVLRALRAEEATKGIRVLILSNSSREQDLEAIKELGVT
                     DYLVKANLSLQELGDRVAQLLEE"
     gene            complement(1665..>3348)
                     /locus_tag="VNU03_24895"
     CDS             complement(1665..>3348)
                     /locus_tag="VNU03_24895"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77785.1"
                     /translation="KSRRGFSPEDISLLSVLGDHASLAIHKARLYDESRVQKEEATKL
                     YEVTAHLATTLDVDSVLDQIVAKTIDLLGCDASGVYVYDEAREGLGFRRGLHLDPTLT
                     RDLVLKPGEGVAGRAFQERRPVSTGDRHADRDLRYTPIAERIMESAPRAYLAVPIEVR
                     DEVLGVLVSYFFEPHEFSEKEVQLLSSLAAHAAIAMDNARHYQEVKLQQSRLAQIFDA
                     TSDGMLLVAPDGRVETANRRAGDLLALKAADLVGIELTEVLAWHRAEGQDYQRMFAAL
                     RSLVDDPDHGVEGDVELRALKRILHWVSQPTKDSTGEPCGFTLTFHDVTHEREVSQMK
                     SDFVSFVTHQLRTPLAGIRWMLELAAQEPGIPVDASSYIQDAREAAQRLIQLVNDLLD
                     ISRLERGKLTVATTPVDLEALTRDVLAETAVLVQEKGHRVSLAGAAVATPVAADAQLL
                     RQVVMNLVSNAIKYTPDDGAIEIRLEPDAGMLRWSIRDSGIGVPLSSQARLFEKFYRA
                     ENVATLETEGTGLGLYLVRLIMEQLDGRVWCESEEGAGATFLFTLPLAAPGA"
BASE COUNT          481 a         1208 c         1130 g          529 t
ORIGIN      
        1 ccaccttctc gccccactgc gtcggcacca ccgagacccg caggtccacc acgaagccgg
       61 agagatccgc ctcgaaccgt ccgtcctgtg cgatgcggtg ctcgtcgatg cgcatggcgc
      121 ccaggatctt gatgcgggcc accagcgacg gatgggtggc gggggccagg ctcagcacct
      181 cgtggagcac cccgtcgacc cggtaacgca ccagggtctc gagctcgtag ggctcgatgt
      241 ggatgtcgga ggcgcgggtg accaccgcgt actcgaggat ccgggtgacc aggtcggccg
      301 ccgaggcctc cgcgggcgcg ccgcgccgcg cggccggatc cgccgcagcg gcgccgtgcc
      361 actgggggcc cggcgcctcc ggccccgccg ccctggccgg ccggttgatc tcggtgctgg
      421 cccggagcgc ggaccgctcg agcatctcgc gcaggttgcc cttgtagagg agctgggcgc
      481 ggcggatcgc gccctcgggg gcgaggtagg gcacgatctc gagcttggtc atctggccca
      541 tctcgcgcac gatgagacgg tcgcgcggat tggccatcgc caccttgagc cggcgctcct
      601 gccgctcgaa gggcatcacc ccgtggctgc gcgcgtattc ctcgcgcagg ctcgacaggc
      661 agtcgagctg gacgtcgccc accttgagct cgatgaagcc cacgtcccac gcctgggcga
      721 gctgggcgag caggaacgag atcgggatgc gcccgcgctc ggcgagcgcg cgctcgagcg
      781 ggatcttgag acgggtcgcc atctgctgcg ccttctcgaa ctcggcggtc tcgaccagct
      841 cgagctgatc caccagcagg gtcttgagct cggcgtcggt gacgaacgat cccgccatca
      901 ggcgcccttc ccgatcagct caccgactcg cttgaccaga tcctgcagcg acaggttggc
      961 cttgacgaaa tagccggccg cgcccgcctc catggcctgc ttcacgtcgc tgtcctgccc
     1021 caggttgctg aggatgatca ccgggatggc cgcggtggcg ggatccgcct tcaaagcgcg
     1081 cagcacctcg aagccctgca tcttcggcat gatcaggtcg agcaggatca ggtgcggcgc
     1141 ttcggcccgc gccgtccgca gcgcttcctc gccgtcgacc gcggggagca ccgtgaagcc
     1201 ctgccgcttc agcgcggtct ccgccgcctt gcgaaggaat cgatcgtcct cggccagtag
     1261 aactcgacgc gccgtctcac ccatgggacg tcactcctcc aggagctgcg ccacccggtc
     1321 gcccagctct tgtagcgaca ggttggcctt gaccaggtag tccgtcacgc cgagctcctt
     1381 gatcgcttcc aggtcctgct cgcgcgacga gttcgagaga atcagcaccc ggatcccctt
     1441 ggtggcctcc tcggccctca gcgctcgcag cacctcgatc ccggtcacct tcggcatgag
     1501 tagatccagc aggatcaggt cgggaatctc ggtccgcacc ttcacgagcg cctcctcccc
     1561 gtccgccgcc accgtgaccg cgaagccgcg ctggcggagg ctcgcctcgg cggcgcggcg
     1621 gaggaagcgg tcgtcctcca cgagcaggat ccggccgccg gtcgtcatgc ccccggcgcc
     1681 gcgagcggca gcgtgaacag gaaggtcgcc cccgccccct cctccgattc gcaccagacc
     1741 cggccgtcga gctgctccat gatcaggcgg accaggtaca gcccgagccc ggtgccctcg
     1801 gtctccaggg tggccacgtt ctcggcccgg tagaacttct cgaagagcct cgcctgcgac
     1861 gacaacggga ccccgatccc gctgtcgcga atcgaccagc ggagcatgcc cgcgtccggc
     1921 tcgaggcgaa tctcgatggc tccgtcgtcg ggggtgtact tgatcgcgtt ggagaccagg
     1981 ttcatcacca cctggcgcag gagctgggcg tccgccgcca ccggcgtggc gacggcggct
     2041 ccggcgagcg acacccggtg ccccttctcc tggaccagca ccgcggtctc ggcgagcacg
     2101 tcccgcgtca gcgcttccag atcgaccggc gtcgtggcga cggtcagctt gccccgctcc
     2161 aggcgcgaga tgtcgaggag atcgttcacc agctggatca gccgctgggc cgcctcgcga
     2221 gcgtcctgga tgtacgagga ggcgtcgacc gggatgccgg gctcctgggc ggccagctcc
     2281 agcatccacc ggatgccggc cagcggggtg cggagctgat gggtcacgaa cgagacgaag
     2341 tcggacttca tctggctcac ttcgcgctcg tgggtcacgt cgtggaaggt cagggtgaag
     2401 ccgcagggct cgccggtgct gtccttggtg ggctgcgata cccagtgcag gatccgcttc
     2461 agggcgcgga gctcgacgtc tccctccacc ccgtggtcgg gatcgtcgac cagcgagcga
     2521 agcgccgcga acatccgctg gtagtcttgc ccctccgccc ggtgccacgc gagcacctcg
     2581 gtgagctcga tccccaccag atcggccgcc ttcagggcca ggaggtcccc cgcccgccgg
     2641 ttcgcggtct cgacccgtcc gtccggggcc accagcagca tcccgtcgga cgtcgcgtcg
     2701 aagatctggg cgagacgact ctgctgcagc ttcacctcct ggtagtggcg cgcgttgtcc
     2761 atcgcgatcg ccgcgtgcgc ggcgaggctc gacagcagct ggacctcctt ctccgagaac
     2821 tcgtgcggct cgaagaagta gctcaccagc accccgagca cctcgtcccg gacctcgatg
     2881 ggcaccgcga ggtaggcgcg cggggcggac tccatgatcc gctccgcgat cggggtgtag
     2941 cgcagatccc ggtccgcgtg ccgatcgccg gtgctcaccg gccgccgttc ctggaaggct
     3001 ctccccgcga cgccctcccc cggcttgagc acgaggtcgc gcgtgagggt ggggtcgaga
     3061 tgaagtcccc ggcggaaccc caggccctcg cgggcctcgt cgtacacgta gacccccgac
     3121 gcgtcgcagc cgagcaggtc gatcgtcttc gcgacgatct ggtccaggac cgagtccacg
     3181 tcgagggtcg tcgccaggtg ggcggtgacc tcgtacagct tggtggcctc ctccttctgc
     3241 acccggctct cgtcgtagag acgggccttg tggatggcga gggacgcgtg gtcgccgagg
     3301 acggagagga gcgagatgtc ctccggcgag aagccgcggc ggctcttg
//
LOCUS       DATMCV010001090         3858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_726151, whole genome shotgun sequence.
ACCESSION   DATMCV010001090 DATMCV010000000
VERSION     DATMCV010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726151"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1026)
                     /gene="moeB"
                     /locus_tag="VNU03_24900"
                     /pseudo
     CDS             complement(250..1026)
                     /gene="moeB"
                     /locus_tag="VNU03_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
     gene            complement(1054..1377)
                     /locus_tag="VNU03_24905"
                     /pseudo
     CDS             complement(1054..1377)
                     /locus_tag="VNU03_24905"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            1526..2080
                     /locus_tag="VNU03_24910"
     CDS             1526..2080
                     /locus_tag="VNU03_24910"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HWP77786.1"
                     /translation="MTPRDPIELSDAAKRAMALQDYPRPPEIEGVQLLELKRFTDDGG
                     SFMELARLDRGVAAALPGFECRQVNYSEMDPGVIKAFHIHRRQTDVWFVPPDDKMLVV
                     LLDVRAGSKSPNAQRRLILGDGHSRLLRIPPGVAHGVKNLGRSRGRIIYLVDFQFSPD
                     PDQTEEGRLPWDFAGADVWDVARG"
     gene            2085..3086
                     /gene="rfbB"
                     /locus_tag="VNU03_24915"
     CDS             2085..3086
                     /gene="rfbB"
                     /locus_tag="VNU03_24915"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWP77787.1"
                     /translation="MKILVTGGAGFIGSNFVRHVLTAHRDDAIVNLDKLTYAGNLENL
                     RDVEADPRYRFVRGDIGDGALVREAMRGVDAVVHFAAETHVDRSNLGADDFLRTNVTG
                     TFTLLEAARELKVGRFLAVSTDEVYGSIAQGAAREGDILNPSNPYSASKAAADLLVRA
                     YWTTHHLPVLVTRSSNNFGPYQYPEKVIPLFVTNALEDKPLPLYGDGKNVRDWLYVLD
                     NCAAIDLVLRRGREGEIYNIGGGTEVENVTLTHRILTLLGKPASLITPVTDRPGHDRR
                     YALDSAKIGALGWAPAATFDAALSATVEWYRTHEAWWKPIKSGAFRQYYQTQYAARA"
     gene            3143..>3858
                     /locus_tag="VNU03_24920"
     CDS             3143..>3858
                     /locus_tag="VNU03_24920"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HWP77788.1"
                     /translation="MGRSGLTWIAVALLGVAFSTTAPAAPRDVDRRLEPSRVSPLPSQ
                     VRRVEPAVVGIRVEVEPDRPSAATLGVERAGSGVIFDADQGYVLTVSYLLLDAERIQV
                     SLRDGRKVPARLTGLDLEAGIGVARLEGAGPWPAATLGDSRAVAAGDVTGTVGVSDEG
                     DLVATASRVQSVRSFAAAWEYMLDRAFIVIPHNAAFGGAALVDTAGRVIGVTSLRLGE
                     APHVDLAIPIEQFLAVKDEL"
BASE COUNT          554 a         1368 c         1322 g          614 t
ORIGIN      
        1 tcgggccccg atctgggttc gtcggcgcgg tcgtggtcgg ggcgcggccg gtcgagctgg
       61 tgtgtgagcg tttggaagag gaccggcgct gaccgcgacg gttggcgccg gatcaccttc
      121 gaccggctcg tatagcggct agccgcccgc cttcgcgacg ggcgcgcccg cgggcgcggg
      181 gtgcccccct gaattcccgt tgtcgcccgt caccgcgcac gcatccgccg ccgcggtccg
      241 gtggatcgac agatcggtga tcgtcgggtg ctcgccacag agcgggcact tgggatcgcg
      301 acgcagcttg acctcgcgga agcgcatgcc gagcgcgtcg taggtcaaga ggcgcccgat
      361 caggggatcg ccgatgccga gcagaagctt gacggcctcg gtggcctgta ccactccgat
      421 gatgcctggg agcacgccca ggacgccggc ctcgcttcag ctggggacca ggccgggcgg
      481 cggaggagtc gggtagaggc agcggtagca cgggccctgg ttcggggcga gcacggtggc
      541 catgccctcg aactggaaga tggagccgtg gacgttggtc ttgcctccga gatagcaggc
      601 gtcgttcacc aggtagcggg tgtcgaagtt gtccgagccg tcgatcacca agtcgtagtc
      661 cttgatgacg tccatcacgt tgtcgacgtc gatgcgcatc ggcagcggga tgacgttgac
      721 gtccgggttc agcgcccgga tcatctcggt gcccgactcc accttgggcc ggcccacgtt
      781 cgcggtggtg tggaggatct ggcgctgcag gttcgacacg tcgaccacgt cgccgtccat
      841 gagcccgatc gtcccgacgc ctgccgcggc caggtacacc gcggtcggcg agccgagccc
      901 gcccgcgccg atcagcagca ccttggagcg aagcagcttg cgctggcctt tctcgcccac
      961 ctggttcagc aggaagtgcc ggctgtagcg ctggatctgc tcggcctgga gcggccggtc
     1021 cttcaccacc gggaggcccg attgcgccca cttctggaac ccgccctgga ggttggtgac
     1081 gttctcgtag cccagatcct tgagggcctt ggccgccagc atcgagcgga gcccgctctg
     1141 acagtagagg acgatcgggg tcgtgggctc ggcggcggcg ccgccgatgc ggaactcgag
     1201 gaagccccgg ctgatgttgg tggccggctc gatgaacccg tcccggtact cgtcgggatc
     1261 gcgcacgtcg atcaggacca gcggctcgcc cgcgtcgatc cgcttcttga cctcggccgg
     1321 tccctgctcg ggcactaccc cccgagcgtc cgccagcatc tccttcagcg tcttcattcg
     1381 ccgctccttg gagaaagccc ctgccttttc agccagttca tattaccacc aattccggtc
     1441 ggatcctgcg atccgtgctc gcccggcgtt cgtcactggg caagactgct ataatcagcg
     1501 actaaatgaa agcgccaatc ctctcatgac gccccgcgac cctatcgagc tgtccgacgc
     1561 cgccaagcga gcgatggcgc tgcaagatta cccgcggccc cccgagatcg agggggtcca
     1621 gctcctggag ctcaagcgct tcaccgacga cgggggctct ttcatggagc tggcccgcct
     1681 ggaccgtggc gtggccgcgg ccctgcccgg ctttgagtgc cgccaggtga actacagtga
     1741 gatggatccc ggggtcatca aggccttcca catccaccgc cgccagaccg acgtgtggtt
     1801 cgtgccgccc gacgacaaga tgctggtggt cctcctcgac gtgcgcgccg ggtccaagag
     1861 cccgaacgcg cagcggcggt tgatcctggg agacggccac tcgcgcctcc tgcggatccc
     1921 ccccggggtg gcgcacgggg tgaagaacct cggccggtcc cgcggccgta tcatctatct
     1981 cgtcgatttc cagttctccc cggaccccga tcagaccgag gagggccgcc tgccctggga
     2041 cttcgcgggg gccgacgtct gggacgtggc gcgcggatag ccggatgaag atcctggtca
     2101 ccggcggcgc cggcttcatc ggctcgaact tcgtccggca cgtgctgacc gcgcaccggg
     2161 acgacgcgat cgtcaatctc gacaagctga cctacgcggg caatctcgag aacctccgcg
     2221 acgtggaggc cgatccccgc taccgcttcg tccgcggcga catcggcgac ggcgcgctcg
     2281 tgcgcgaggc gatgcgcggg gtggacgcgg tggtgcattt cgcggccgag acccacgtgg
     2341 accggtcgaa cctgggggcg gacgatttcc tccgcaccaa cgtgaccggc accttcaccc
     2401 tgctggaggc cgcgcgcgag ctcaaggtgg gccgcttcct ggcggtgagc accgacgagg
     2461 tctacgggag catcgcccag ggcgccgccc gcgagggcga catcctgaac ccgtcgaatc
     2521 cgtactccgc ctccaaggcg gccgccgacc tgctcgtgcg cgcctactgg accacgcacc
     2581 accttccggt gctcgtcacc cgcagcagca acaacttcgg gccctaccag tatccggaga
     2641 aggtgatccc gctcttcgtc accaacgcgc tcgaggacaa gccgctgccg ctctacggtg
     2701 acggcaagaa cgtgcgcgac tggctctatg tcctcgacaa ctgcgcggcg atcgatctcg
     2761 tgctccgccg gggccgggag ggcgagatct acaacatcgg cgggggcacc gaggtggaga
     2821 acgtcacctt gacccaccgc atcctgaccc tgctcggcaa gccggcgagc ctgatcacgc
     2881 cggtcaccga ccggcccggt cacgaccgcc gctacgcgct cgactccgcc aagatcggcg
     2941 cgctcggctg ggcccccgcc gcgaccttcg acgccgcgct ctccgcgacc gtggagtggt
     3001 accgcaccca cgaggcgtgg tggaagccga tcaagtccgg cgcgttccgt cagtactacc
     3061 agacccagta cgcggcccgg gcctgaggtc gagcccgccc cccgcgggcg gctcgggcat
     3121 aatgcgcggg gaggtggtcg gcatgggtcg ctccggactc acctggatcg cggtggcgct
     3181 gctgggcgtc gccttctcca ccaccgctcc cgccgctccg cgcgacgtcg accgccgcct
     3241 cgagccctcc cgggtgtccc cgctgccgag ccaggtccgc cgggtcgagc ccgcggtggt
     3301 cggcatccgg gtcgaggtcg agcccgaccg gccgtccgcg gccacgctcg gagtcgagcg
     3361 cgcgggcagc ggggtcatct tcgacgccga ccagggctat gtcctgacgg tgagctacct
     3421 gctgctcgac gccgagcgca tccaggtctc gcttcgtgac ggccgcaagg tgcccgcgcg
     3481 gctgaccggg ctcgatctcg aggccggcat cggagtcgcc cgcctggagg gcgccggccc
     3541 gtggccggcg gccaccctcg gcgactcgcg cgcggtggcc gcgggggacg tcaccgggac
     3601 ggtgggcgtc tccgacgagg gcgacctggt ggccaccgcc agccgcgtgc agtcggtgcg
     3661 gtcgttcgcg gccgcgtggg agtacatgct ggatcgcgcg ttcatcgtga tcccgcacaa
     3721 cgcggccttc ggcggggcgg cgctcgtcga caccgccgga cgcgtgatcg gcgtcacctc
     3781 gctgcgtctc ggggaggcgc cccacgtcga cctcgccatc ccgatcgagc agttcctggc
     3841 cgtcaaggac gagctgat
//
LOCUS       DATMCV010001091         2271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_860258, whole genome shotgun sequence.
ACCESSION   DATMCV010001091 DATMCV010000000
VERSION     DATMCV010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860258"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VNU03_24925"
     CDS             complement(<1..689)
                     /locus_tag="VNU03_24925"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP77789.1"
                     /translation="MPDVFIDEVADHTMAMFLAAHRRLRLMHQLTVDNKWTEGRPYFT
                     NIPRLYGQTIGLISFGNVAKAVARRCHAFGLRVIAYDPFLAELEMTAVGVEPVTSLIE
                     LCQRADFLSMHAPLNSETRHMMSEREFRAMKKSAIFINNGRGPTVDEKALSEALRQGW
                     IAGAALDVFEVEPVDPNNALLHMDNVIVTPHIASATARMAPETRRRLGREIATVLQGK
                     WPRSAVNPGVLA"
     gene            complement(611..1012)
                     /locus_tag="VNU03_24930"
     CDS             complement(611..1012)
                     /locus_tag="VNU03_24930"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77790.1"
                     /translation="MAAASAKFKVVVQKPSGGITYDLADSAYAIEREALDPIGAEIVE
                     VPAKTEEEFIAAAKDADAVIARNRRITAAIIKGLRNCKVIGLGSVGADTVDVDAATEA
                     GIVVTTCPTCSSTRWPTTPWPCSSPRIAACG"
     gene            1123..1908
                     /locus_tag="VNU03_24935"
     CDS             1123..1908
                     /locus_tag="VNU03_24935"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77791.1"
                     /translation="MLVGAFAVAHTPQLLIRPETEDRALVLRVHAAMARVKARLEALR
                     PDAIAVIAGDHVEAFFLDAVPALAVYVGRECAGTFGPYRYRFDIHEELARAVVEQGIE
                     RGFDLLYTQDAKLDYAFYVPLHFAMPEPARPIVPLHVNVYLPPQPTPARCHAWGQALG
                     EILRARPERVALIASGGMSHFPGTDRYASPEFEYDRGLLEVLADGRGRELARLTGEEL
                     DKRGNIELRTWITLLGAVGDARAQVYCYEPSWHHGNAVVEWPL"
     gene            1916..2194
                     /locus_tag="VNU03_24940"
     CDS             1916..2194
                     /locus_tag="VNU03_24940"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77792.1"
                     /translation="MSETAHYRFPPSSAYALNRCLYALKSDREFAERFVADPAAATGA
                     LGLTEGERADLHRMDRDALVARGAHPYLVFMAGLRLKMLAEPGALEYF"
BASE COUNT          307 a          811 c          780 g          373 t
ORIGIN      
        1 gcgaggacgc ccgggttgac ggccgagcgg ggccacttgc cctgaaggac ggtggcgatc
       61 tcgcggccga ggcggcggcg cgtctcgggc gccatgcggg ccgttgccga tgcgatgtgc
      121 ggcgtcacga taacgttgtc catgtggagc agcgcgttgt tcggatccac cggctccacc
      181 tcgaacacgt cgagcgcggc gcccgcgatc cagccctggc gcagcgcctc gctgagggcc
      241 ttctcgtcca ccgtcggccc ccgcccgttg ttgatgaaga tcgcgctctt cttcatggcc
      301 cggaactcgc gctcgctcat catgtggcgc gtctccgagt tcagcggcgc gtgcatcgac
      361 aggaagtcgg cgcgctggca cagctcgatc aggctcgtca ccggctcgac tcccaccgcg
      421 gtcatctcga gctcggccag gaacggatcg taggcgatca cccgcaggcc gaaggcgtgg
      481 cagcggcgcg cgaccgcctt ggccacgttg ccgaacgaga tgaggccgat ggtctgcccg
      541 tagaggcgcg ggatgttcgt gaagtagggc cgcccctccg tccacttgtt gtcgacggtg
      601 agctggtgca tcagccgcag gcggcgatgc gcggcgagga acatggccat ggtgtggtcg
      661 gccacctcgt cgatgaacac gtcgggcacg ttgtgaccac gatgcccgcc tcggtggccg
      721 cgtccacgtc caccgtgtcc gcgcccacgc tgccgagccc gatgaccttg caatttcgca
      781 ggcccttgat gatggccgcg gtgatgcgcc ggttgcgcgc gatgaccgcg tcggcgtcct
      841 tcgcggccgc gatgaactcc tcctcggtct tggccggcac ctcgacgatc tcggctccga
      901 tggggtcgag cgcctcgcgc tcgatcgcgt aggccgaatc ggccaggtca taggtgatcc
      961 cgcccgacgg cttctggacg acgaccttga acttcgcgct tgcggctgcc atgccggcga
     1021 cctcctgagt ggtctcagtg tgggatttcc gtctgcggcc accaagcata cgcttcgccc
     1081 cgacctgaag tcgaggccct cttgtgctag ggtttccctg ccatgctcgt cggcgccttc
     1141 gcggtcgcgc acaccccgca gctcctgatc cgaccggaga ccgaggaccg cgcgctggtc
     1201 ctccgcgtgc acgccgcgat ggcgcgggtc aaggcgcggc tcgaggcgct ccggccggac
     1261 gcgatcgcgg tgatcgccgg cgaccacgtc gaggcgttct ttctcgatgc ggtgccggcg
     1321 ctggcggtct acgtgggccg cgagtgcgcg ggcaccttcg gcccctaccg gtaccgcttc
     1381 gacatccacg aggagctggc ccgcgcggta gtggagcagg gcatcgagcg cggcttcgac
     1441 ctgctctaca cccaggacgc caagctcgac tacgcgttct acgtgccgct gcacttcgcg
     1501 atgccggagc ccgcgcggcc catcgttccc ctgcacgtca acgtgtacct gccgccccag
     1561 cccaccccgg cccgctgcca cgcctggggc caggccctcg gcgagatcct gcgcgcgcgc
     1621 ccggagcgcg tcgcgctcat cgcctcgggc gggatgtccc acttcccggg caccgaccgc
     1681 tacgcctctc ccgagttcga gtacgaccgc gggctgctcg aggtgctggc cgacgggcgg
     1741 gggcgggagc tcgcccggct gaccggcgag gagctcgaca agcggggcaa catcgagctg
     1801 cggacctgga tcaccttgct gggcgcggtg ggcgacgcgc gggcgcaggt ctactgctac
     1861 gagccctcgt ggcatcacgg caacgcggtg gtggagtggc cgctctgagc ccgcgatgag
     1921 cgagaccgcg cactaccgct tcccgccgtc gagcgcctac gcgctgaacc ggtgcctcta
     1981 cgcgctcaaa tcggaccggg agttcgcgga gcgcttcgtc gccgacccgg cggccgccac
     2041 cggcgcgctg gggctgaccg agggcgagcg ggccgacctg caccgcatgg atcgcgatgc
     2101 cctggtggcc cgcggcgccc atccctacct cgtattcatg gccggactcc ggctcaagat
     2161 gctcgccgag cccggggctc tggagtactt ctaggggagt acttctaggc gagcacgtct
     2221 aacctaggcg agacgtctag ccgccggtcc tcacctcgag gcgagtgccg a
//
LOCUS       DATMCV010001092         3744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_57929, whole genome shotgun sequence.
ACCESSION   DATMCV010001092 DATMCV010000000
VERSION     DATMCV010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57929"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..704
                     /locus_tag="VNU03_24945"
     CDS             168..704
                     /locus_tag="VNU03_24945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77793.1"
                     /translation="MRGLVLAAGAFALLVLASAVMLRVYPGNKEFKIFLGAFAGSVVL
                     YTAGFWLLPADLGFIPPARQESSPTVDLGNGLLILVLVFHGYWCFAYFSCLSPSMSVM
                     VELRQRGSEGMTESEALAIHGSGEPVNLIFRRRLPKLLKGGYVTEEDSAYRLLAPGQR
                     VASLGSFLKRLINAQVGA"
     gene            734..1240
                     /locus_tag="VNU03_24950"
     CDS             734..1240
                     /locus_tag="VNU03_24950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77794.1"
                     /translation="MSGTLMTLWLVVQGVVFHAAAPRKAFSSMVGWFAPTVPLYLVLY
                     WMTPANLGVLSDGFVGADWRLGLLNGLIIHVLLFLTAGLFYSHADRSITVRLLIELSR
                     APRQRLTLTQMQEMCGVEVLMQDRLGIMVRNGFLVARGNRFVLTPKGWIGGSVGLVAR
                     RVFRMKAL"
     gene            1253..2296
                     /locus_tag="VNU03_24955"
     CDS             1253..2296
                     /locus_tag="VNU03_24955"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWP77795.1"
                     /translation="MDATETSRKGPGLDEMTVGAPKSAREALQSGAISLEALRAAHQM
                     RDWRSALDVLGTLASFIAVPVLFGLHPREWTFVLLALCAIRNFNCAAQLVHESDHGTM
                     FRDPRLNRGLGNLFAYLLGYTRSGHRTAHMDHHLYLNTERDPDIIFSQPSAPASAVLR
                     GLLSDLFLVSAIKRLLQYSQSDRATFTVSPWRRLTPAYFARMAGSMVPVAVTQLVLLG
                     IYTLAGGPWAYLLLHVLPIMTLYPLQIRVRSIAEHGFEAGYRPSSPQEAWMTRTSRLN
                     VLERWIIAPLGQYLHYEHHVFPSIPNYNLARVHRLLMAAGIPVPTNRSYFGFVWNRLR
                     TEWRGGAVARESA"
     gene            2293..3222
                     /locus_tag="VNU03_24960"
     CDS             2293..3222
                     /locus_tag="VNU03_24960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP77796.1"
                     /translation="MSRSVQDAQLTGAVCDLCRSDRHAVPVLEAEDYEYGVPGRFTLS
                     HCTACDLYYQTPRPDRDVLPTYYPPTYAVYGDDPVVGWLFRLTYWLDARRVARLIGPR
                     GRVLDVGCGAGGALTALAGAGKWEVCGLELDVEAARHAARLGFNVRQGDLVDTDFPPG
                     SFDLIRMGHVIEHVRDPIATLQRAWQLLRPGGTLFGETPNIDCWDFRLFGRYWGALHF
                     PRHIALFSPETITRACEQAGFVGTRIIPRLRTVGWSAGIQNWLADRGLRVPANGRVRW
                     YPALIAPFLPVTVLQSLVSRTATMAFVARRPEA"
     gene            3224..>3744
                     /locus_tag="VNU03_24965"
     CDS             3224..>3744
                     /locus_tag="VNU03_24965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP77797.1"
                     /translation="MALRYATELGAFDRIKNDLAFQVRRRIFERFMRECAPGPEARVA
                     DFGVSGHRDHPVHYFFETLYPHRDRLTVIGRAAEDAGWFPEAFPGLRFLEADLREIRL
                     PDGYFDYGICNAVVEHAGTRAQQLALVSEVCRVCRCVLFTTPNKRFPVELHTWLPLLH
                     WLPDPTYRAALRRL"
BASE COUNT          518 a         1303 c         1255 g          668 t
ORIGIN      
        1 tcatcgtggg gagcatccgc cacatcgcgg acatcagcga agcgttccag agcggcgccg
       61 acatcgtgac ggtgccgccc aagttcttcc gcgcgatggt gacccatccc aagaccgacg
      121 aagcggtgaa gcagttcgtc accgacttcc agaagtggat gcagtaggtg aggggtctcg
      181 tgctcgccgc gggcgccttc gcgctgctgg tgctggccag cgcggtcatg ctgcgcgtct
      241 accccgggaa caaggagttc aagatcttcc tgggcgcgtt cgcgggctcg gtcgtcctct
      301 acacggccgg cttctggcta ctgcccgcgg atctgggatt catcccgccc gcgcggcagg
      361 agtcgtcccc cacggtggat ctgggcaacg gcctcctgat cctggtcctg gtgttccacg
      421 gctactggtg cttcgcgtac ttttcctgtc tgagcccctc catgagcgtc atggtcgagc
      481 tccgccagcg gggctccgag gggatgaccg agtcggaggc cctcgcgatc cacgggagtg
      541 gcgagccggt gaacctcatc ttccggcggc ggctacccaa gctgctgaag ggcggctacg
      601 tgaccgaaga ggacagcgcc tatcggctcc tggcccccgg gcagcgggtg gcatcgctcg
      661 ggtcctttct caagcgactg atcaacgccc aggtcggcgc gtaacggacg cggacgtgct
      721 caaagggctc ctcttgagcg ggacgctgat gacgctgtgg ctggtggtcc agggcgtcgt
      781 gttccacgcg gccgctcccc ggaaggcgtt cagctcgatg gtcggttggt tcgcgccgac
      841 ggtccccctg tatctcgtcc tctactggat gacccccgcc aacctcggtg ttttgtcgga
      901 cgggttcgtg ggagcggact ggcggctcgg cctgctgaac gggctgatca ttcacgtgct
      961 gctcttcctg accgcggggc tcttctactc ccatgcggac cggtccatca cggtgcggct
     1021 cctgatcgag ctgtcccggg cgccccggca acgactcacc ctcacacaga tgcaggagat
     1081 gtgcggggtc gaggtgctga tgcaggaccg cctcggcatc atggtgcgca acggattcct
     1141 ggtcgcacgc ggcaaccggt ttgtgctcac tcccaaggga tggatcggcg gcagcgtggg
     1201 gctggtggct cgccgagttt ttcgaatgaa ggcgctctga ggacaccatc acatggacgc
     1261 gaccgagacg agccgcaagg gaccgggcct cgacgagatg acggtggggg cgcccaagtc
     1321 tgcccgggag gctctccagt cgggggcgat cagcctggag gcgctccggg ctgctcacca
     1381 gatgcgggat tggcgtagcg cgctggacgt cctcggcacc ctcgcctcgt tcatcgcggt
     1441 tccggtgctg ttcggcctcc acccgcgcga gtggaccttc gtgctgctcg ccctctgcgc
     1501 gatccgcaac ttcaactgcg ccgcgcagct cgtccacgag agcgatcacg ggacgatgtt
     1561 ccgggatcca cgcctgaacc ggggcctcgg gaacctcttc gcgtacctgc tcggctacac
     1621 ccggagcgga caccggactg cgcacatgga tcatcacctg tacctcaaca ccgagcgcga
     1681 tccggacatc atcttcagcc agccgagcgc gccggcatcg gcggtgctcc gcgggctcct
     1741 gagcgatctg ttcttggtct ccgcgatcaa gcgcctgctc cagtactcgc aatcggaccg
     1801 ggccaccttc acggtgtcgc cctggcggcg cctgaccccg gcctacttcg cgcggatggc
     1861 cgggtcgatg gtgccggtgg cggtgaccca gctggtgctc ctcggcatct acaccctggc
     1921 gggcggaccg tgggcctacc tgctcctgca cgtcctgccg atcatgacgc tctacccgct
     1981 ccagatccgc gtgcgctcga tcgccgagca cggcttcgag gccggctatc gtccgagctc
     2041 gccccaggag gcgtggatga cccgcaccag ccggctgaat gtcctcgagc ggtggatcat
     2101 cgcgcctctc ggccagtacc tgcactacga gcatcacgtg tttccgagca tcccgaacta
     2161 caacctggcc cgagtacacc gtctcctgat ggcggccggc atcccggtgc ccacgaaccg
     2221 cagctacttc ggcttcgtct ggaaccgcct gcggaccgaa tggcgggggg gcgcggttgc
     2281 ccgggagagc gcatgagccg ctcggtgcag gacgctcagc tcaccggggc ggtgtgcgat
     2341 ctgtgccgct ccgatcgcca cgcggtgccc gtcctcgagg cggaggacta cgagtacggg
     2401 gtgccgggcc gattcaccct gtcgcactgc accgcctgcg acctctacta ccagacgcca
     2461 cgcccggatc gcgacgtgct tcccacgtac tacccgccga cctacgcggt ctacggtgac
     2521 gatccggtcg tcggctggct cttccgcctg acctactggc tcgacgcgcg gcgggtggct
     2581 cgcctgatcg gcccgcgggg ccgcgtgctc gacgtgggct gcggcgccgg gggtgcgctg
     2641 accgcgctgg ccggggcggg gaagtgggag gtctgcgggc tcgagctgga cgtggaggcg
     2701 gcgcggcacg ccgcccggct gggcttcaac gtccggcagg gggacctcgt tgataccgat
     2761 ttccctcccg ggtccttcga cctgatccgc atgggccacg tcatcgagca cgtgcgcgac
     2821 ccgatcgcga cgctccagcg cgcgtggcag ctcctccgtc cgggcgggac gctgttcggc
     2881 gagaccccga acatcgactg ctgggacttc cgcctcttcg ggcgctactg gggcgcgctc
     2941 cactttcccc ggcacatcgc gctcttcagc cccgagacga tcacccgagc ctgcgagcag
     3001 gcggggttcg tgggcacgcg gatcatcccc cgcctccgca cggtgggctg gagcgcgggc
     3061 atccagaact ggctggccga ccgcggcctc cgggtgccgg ccaacgggcg ggtccgctgg
     3121 taccccgcgc tgatcgcacc tttcttgccg gtgaccgtcc tgcaatcgct ggtgagccgc
     3181 accgcgacca tggccttcgt ggcgcgtcgc ccggaggcct gacgtggccc tccggtacgc
     3241 gaccgagctc ggtgccttcg atcggatcaa gaacgacctg gcgttccagg tccggcggcg
     3301 tatcttcgag cggttcatgc gggagtgcgc tcctggcccg gaggcgcgcg tcgccgactt
     3361 cggggtgtca ggccaccgcg accatccggt gcactacttc ttcgagaccc tctacccgca
     3421 tcgtgatcgg ctcaccgtga tcggccgcgc cgccgaagat gcgggctggt tcccggaggc
     3481 gtttccggga ctgcgcttcc tggaggcgga cctgcgcgag atccggctgc cggacgggta
     3541 cttcgactat ggcatctgca atgcggtggt cgagcacgca ggcacgcgcg cccagcagct
     3601 cgcattggtg tcggaggtct gtcgggtgtg ccgctgcgtg ctattcacga cgcccaacaa
     3661 gcggtttccg gtggagctgc acacctggct acccctgctc cactggctgc cggatcccac
     3721 ctaccgcgcg gccctccgac gtct
//
LOCUS       DATMCV010001093         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_661713, whole genome shotgun sequence.
ACCESSION   DATMCV010001093 DATMCV010000000
VERSION     DATMCV010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661713"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VNU03_24970"
     CDS             complement(<1..843)
                     /locus_tag="VNU03_24970"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWP77798.1"
                     /translation="MAPIDVTGLRQPLPDASHAPGALYASPEFYRLDVEKLFMRDWLF
                     VGRVEELPDPGDYLTMRIAGEPIIVARTPAGELAAYYNMCLHRGVEVAEGCGNTAAFK
                     CPYHGWIYDLSGRLTGAAYMKESEGFELGEQRMRPLRLETWRRNIFVAFDPAAPPLHE
                     FVAELEQDFAFLRMEDCRLGNRIVLDLECNWKFVHENLMDFYHVGVLHAKTFGARFSW
                     TDDNVHLKPGGGISIFYAAGPPTPGAEPLLGKMPWLEDRPVSFACTGFLGPNCTLFGR
                     IDCVR"
     gene            complement(890..1330)
                     /locus_tag="VNU03_24975"
     CDS             complement(890..1330)
                     /locus_tag="VNU03_24975"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWP77799.1"
                     /translation="MAKATQATAFPKGHDRWTEAQANALLRAVEDMFHRRDVDALVHG
                     FTEDCVVRFAEQPARRGRAALRQLFTARLARQKDYRLTKRLMALDDNVLANTWEGTWE
                     DRETGRAMAGRGVEVWTMRDGMIAVWDAAFNIWEQGGERKSPVM"
     gene            complement(1370..2011)
                     /locus_tag="VNU03_24980"
     CDS             complement(1370..2011)
                     /locus_tag="VNU03_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HWP77800.1"
                     /translation="MRIVRVRPRLVTSLGVGIAAALALTALTGWRPVMRGLVGWDLGV
                     GLYVVLACEMMARADVQQIRRRAADQDEGAIVILALTVAAALASLLAIIALLGTSAAG
                     PSPRRSGELVLATITIVLSWALIHTIFALHYAHEFYDGSGVGGMAFPGGEKEPDYWDF
                     AYFSLVVGMTSQVSDVGVTSKVIRRTVAAHGVVSFFFNAALLALTVNIAASAI"
     gene            complement(2026..>2208)
                     /locus_tag="VNU03_24985"
     CDS             complement(2026..>2208)
                     /locus_tag="VNU03_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807873.1"
                     /note="with GsiABD is involved in the transport of
                     glutathione into the cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HWP77801.1"
                     /translation="GLGVQPPSPEWGAMLSNARAYMITAPHVATVPGLAIMLVVLGFN
                     LLGDGLRDALDPRLRE"
BASE COUNT          352 a          775 c          757 g          324 t
ORIGIN      
        1 gcgcacgcag tcgatgcggc cgaacagcgt gcagttgggg ccgaggaacc ccgtgcaggc
       61 gaagctgacc ggccggtcct cgagccacgg catcttcccc agcaggggct cggccccggg
      121 cgtcgggggt ccggccgcgt agaagatgga gatccccccg cccggcttga gatgcacgtt
      181 gtcgtccgtc cagctgaacc gggccccgaa ggtcttcgcg tgcagcacgc ccacgtgata
      241 gaagtccatc aggttctcgt gcacgaactt ccagttgcac tcgagatcga gcacgatccg
      301 attgccgagc cggcagtcct ccatccgcag gaacgcgaag tcctgctcga gctcggcgac
      361 gaactcgtgg agcggcgggg cggcggggtc gaaggccacg aagatgttcc gccgccaggt
      421 ctcgagccgc aggggccgca tgcgctgctc tccgagctcg aatccttcgc tctccttcat
      481 gtacgccgcg ccggtcaggc ggccggagag atcgtagatc cagccgtggt agggacactt
      541 gaaggccgcc gtgttgccgc acccctcggc gacctcgacc cctcggtgaa ggcacatgtt
      601 gtagtacgcg gcgagctcgc ccgccggggt gcgggcgacg atgatgggct cgcccgcgat
      661 gcgcatggtc aggtagtcgc ccggatccgg cagctcctcg acgcggccga caaagagcca
      721 atcccgcatg aagagcttct cgacgtcgag ccgatagaac tccggggagg cgtagagcgc
      781 tccgggcgcg tggctggcgt cgggcagcgg ctggcgcagc ccggttacgt cgatgggggc
      841 catggctcct cctcaggtgc ggtgcggtgt cggcaccgcg ccggaatcat cacatcacgg
      901 gcgacttccg ctcgcctccc tgctcccaga tgttgaaggc cgcgtcccag accgcgatca
      961 tcccgtcgcg catggtccac acctcgaccc cgcggcccgc catcgctcgc ccggtctccc
     1021 gatcctccca ggtgccttcc caggtattgg ccagcacgtt gtcgtccagg gccatcagcc
     1081 tcttggtgag ccggtagtcc ttctggcggg cgagccgggc cgtgaacagc tgccgcagcg
     1141 cggcgcgccc ccgccgcgcc ggctgctcgg caaaccgcac gacgcagtcc tcggtgaacc
     1201 cgtgcacgag cgcgtccacg tcacgccggt ggaacatgtc ctccaccgcc cgtagcaagg
     1261 cgttcgcctg cgcttccgtc caccggtcgt gccccttcgg aaacgcggtt gcctgcgtcg
     1321 ccttcgccat cgcgcgcctc ctcacacggc ccgggtggcc gctccggccc tatatcgcac
     1381 tcgcggcaat gttcaccgtg agggcgagga gcgcggcgtt gaagaagaac gacaccacgc
     1441 cgtgtgcggc gaccgtccgg cggatcacct tgctcgtgac gcccacgtcg gagacctgcg
     1501 aggtcatgcc gacgaccagc gagaagtagg cgaagtccca gtagtccggc tccttctcac
     1561 cgcccgggaa cgccatgccg ccgacgccgc tcccgtcgta gaactcgtgg gcatagtgca
     1621 gcgcgaatat cgtgtggatg agagcccagg acagcacgat cgtgatcgtc gcgaggacca
     1681 gctcgccgga ccgtctcggt gacggtccgg ccgcggaggt gccgagcaag gcgatgatgg
     1741 cgaggaggct cgccagggcc gcggcaacgg tcagagccag gatgacgatc gcgccctcgt
     1801 cctgatccgc ggcgcggcgg cggatctgct gcacgtccgc ccgtgccatc atctcgcagg
     1861 cgaggaccac gtagagcccg accccgaggt cccagccgac gaggccgcgc atcaccggtc
     1921 tccagccggt cagcgcggtg agggccaggg cggccgcgat gccgaccccg agcgaggtga
     1981 ccagtcgcgg ccggactcga acgatccgca acacccgggg agttttcact cgcgcaggcg
     2041 aggatcgagc gcgtcgcgca ggccatcgcc gagcaggttg aagccgagca ccaccagcat
     2101 gatggccagg ccggggacgg tggcgacgtg gggcgcggtg atcatgtagg cgcgcgcatt
     2161 ggagagcatc gcgccccact cggggctggg aggctggacg ccgaggcc
//
LOCUS       DATMCV010001094         2841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_195601, whole genome shotgun sequence.
ACCESSION   DATMCV010001094 DATMCV010000000
VERSION     DATMCV010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195601"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VNU03_24990"
     CDS             <1..1470
                     /locus_tag="VNU03_24990"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWP77802.1"
                     /translation="GSMSTSVLVGVDIGTSSAKGAAYDPDGRCLGTTEIAYRYETPRR
                     GWAEADAGAWWRTTCRILRSLADTVRPAQIDGVAVTGQAPTLLAVDAAGRPVRPAILW
                     LDVRSEAMAEEIAARVGPEAERVSGNRVHAFYLGPKLAWLRRHEPARFERPLTILQSH
                     SYPVMRLTGARVTDYSSAALAAPLYDCRARAWSPEMCAALGVDPAWLPEIKPSHAIAG
                     RVTAAAARETGLREGTPVAVGGADFAASTLAAGVTEPGEACLMLGTAGNLIMPLATPD
                     FDTRLINSHHVGCDRYLALGGTLSGALQEWFRSVAAPNVAFETLDAEAAAVPAGSDGL
                     RLLPYLQGERTPVWDAGACGAFVGLSLVHGRGHLYRAVLEGVAVSFRHCAEIAAEKGL
                     LLREVIAVNGGARSAVWRQILCDALGVPLLYVPDGAGAPTGGALLAGMAVGLVDGVAT
                     ARRWRSALVRHAPDAARTETYAGLLAGRRELYPAIRQIA"
     gene            1470..2516
                     /locus_tag="VNU03_24995"
     CDS             1470..2516
                     /locus_tag="VNU03_24995"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HWP77803.1"
                     /translation="MARIAILGAGFMGSALTVPATDNGHTVALWGTHLDDHLIAAVRA
                     GAKHPKLGLVLPAGVTAYDSTELPRALAGADLVISAVTSDGALPVLRRAATRLAPGVP
                     VLSVSKGLAQSRGRAVTLSDALADGAGLHIVTVGGPSKALELARRVPTAVVYASPQAA
                     VRRRLRQMLETAYYRVEESADQRGLELCSALKNAYAIAIGLCDGLVAAGRAEAMYNTK
                     SALYTQALREIARLGLTVKMRGATVHGLGGAGDLHVTGMAGRNRVFGELRGAGRPTKE
                     VVATLKARDELTEGYAAIRWCWRFARERKVTGVPLLHALHRIVYGGHDVERELSAACF
                     GSASPSTRSKGGRS"
     gene            2513..>2841
                     /locus_tag="VNU03_25000"
     CDS             2513..>2841
                     /locus_tag="VNU03_25000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77804.1"
                     /translation="MRRMLAVVLGLGLTWMAGAGPAAADPIVAQFWYSFGGKNREVTE
                     AHIKKFNESQSKYRIEGTFQGDYFQALAKIRAAAATKTAPAVFHVIGEAMPQLWQSGL
                     FENLEPYA"
BASE COUNT          343 a         1044 c         1074 g          380 t
ORIGIN      
        1 ggctccatgt ccacctccgt cctggtcggc gtcgacatcg gcacgtcatc cgccaaggga
       61 gccgcctacg atccggacgg ccgctgcctc ggcactaccg agatcgcgta tcgctacgag
      121 acgccgcggc gtggctgggc cgaggcggac gcgggggcgt ggtggcgcac gacgtgtcgc
      181 atcctccgct cgctcgccga cacggtgcgg cccgcgcaga tcgacggggt ggcggtgacc
      241 ggccaggccc cgacgctgct cgcggtggac gcggcgggcc gcccggtgcg ccccgccatc
      301 ctctggctcg acgtgcggag tgaggcgatg gcggaggaga tcgcggcgcg cgtcgggccc
      361 gaggcggagc gcgtctccgg caaccgcgtc cacgccttct atctgggccc caagctcgcg
      421 tggctgcggc gccacgagcc ggcccgcttc gagcgcccgc tcacgatcct ccagtcgcac
      481 agctatccgg tgatgcggct caccggcgcc cgggtcaccg actactcctc cgccgcgctc
      541 gccgcgccgc tttacgattg ccgggcgcgc gcgtggtcgc ccgagatgtg cgcggcgctg
      601 ggcgtcgatc ccgcgtggct gcccgagatc aagccgagcc acgccatcgc gggccgggtg
      661 accgcggccg ccgcccggga gaccgggctg cgcgagggca ccccggtcgc ggtgggcggc
      721 gccgacttcg cggcctccac cctggccgcg ggcgtcaccg agccggggga ggcgtgcctg
      781 atgctgggca ccgcgggtaa cctgatcatg ccgctcgcca cgcccgactt cgacacgcgg
      841 ctgatcaact cgcaccacgt ggggtgcgac cggtacctcg cgctcggggg cacgctctcg
      901 ggcgcgctgc aggagtggtt ccgctccgtc gcggcgccca acgtcgcgtt cgagacgctc
      961 gacgcggagg cggccgcggt gcccgcgggc tccgacggcc tgcgtctgct gccgtacctg
     1021 cagggcgagc gcacgccggt gtgggacgcg ggcgcgtgcg gggccttcgt cggcctctcg
     1081 ctggtgcacg ggcgcgggca cctctaccgc gcggtgctcg agggcgtggc ggtgtccttc
     1141 cgccactgcg cggagatcgc ggccgagaag ggcctgctcc tccgcgaggt catcgcggtg
     1201 aacgggggcg cgcgcagcgc ggtgtggcgc cagatcctgt gcgacgccct cggcgtgccg
     1261 ctcctctacg tccccgacgg ggcgggcgcg ccgaccggtg gcgcgctgct cgcgggcatg
     1321 gcggtcgggc tggtcgacgg ggtggcgacc gcccggcgct ggcgcagcgc gctggtgcga
     1381 cacgcccccg acgcggcccg caccgagacc tacgcgggcc tgctcgcggg ccggcgcgag
     1441 ctctacccgg cgatccgcca gatcgcctga tggcccgcat cgccatcctc ggggccggct
     1501 tcatgggctc ggccctgacg gtgcccgcga ccgacaacgg ccacaccgtc gcgctctggg
     1561 gcacgcacct cgacgaccac ctgatcgcgg cggtgcgcgc cggggccaag catcccaagc
     1621 tcggtctcgt cctgcccgcg ggggtgaccg cgtacgattc gaccgagctg ccgcgggcgc
     1681 tcgccggcgc ggacctcgtg atcagcgcgg tgacgtcgga cggcgcgctc ccggtgctgc
     1741 gacgcgcggc gacccggctg gccccagggg tgccggtgct gagcgtgtcc aagggcctgg
     1801 cccagagccg cggccgggcg gtcacgctct ccgacgcgct cgcggatggg gcgggcctcc
     1861 acatcgtcac ggtgggcggg ccgtcgaagg cgctcgagct ggcccggcgc gtgcccaccg
     1921 cggtggtcta cgcctccccg caggccgcgg tgcgccggcg cctccggcag atgctcgaga
     1981 cggcctacta ccgcgtcgag gagagcgccg accagcgcgg gctcgagctc tgctccgcgc
     2041 tcaagaacgc ctatgccatc gcgatcgggc tctgcgacgg cctggtggcg gcgggccggg
     2101 ccgaggcgat gtacaacacc aagtccgcgc tctacaccca ggcgctccgc gagatcgcgc
     2161 ggctgggcct caccgtgaag atgcgcgggg ccaccgtcca cggcctgggc ggggcgggcg
     2221 acctgcacgt caccggcatg gccgggcgca accgcgtctt cggcgagctg cgcggcgcgg
     2281 gccggccgac caaggaggtc gtggccacgc tcaaggcgcg cgacgagctg accgaggggt
     2341 acgccgcgat ccgctggtgc tggcggttcg cgcgcgagcg gaaggtgacg ggcgtgccgc
     2401 tgctccacgc cctccaccgc atcgtctacg gtgggcacga cgtggagcgc gagctctccg
     2461 cggcctgctt cgggagcgca tctccgtcca cccgatccaa aggaggcagg tcatgaggcg
     2521 gatgctggcg gtcgtgctgg ggttggggtt gacgtggatg gcgggcgcgg ggccggcggc
     2581 ggcggatccc atcgtggccc agttctggta ctcgttcggg ggcaagaacc gcgaggtcac
     2641 cgaggcccac atcaagaagt tcaacgagtc gcagtccaag taccggatcg agggaacgtt
     2701 ccagggcgac tacttccagg ctctggccaa gatccgagcg gcggcggcga ccaagactgc
     2761 ccccgccgtc ttccacgtga tcggcgaggc catgccccag ctctggcagt cggggctctt
     2821 cgagaacctc gagccctacg c
//
LOCUS       DATMCV010001095         4971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_794470, whole genome shotgun sequence.
ACCESSION   DATMCV010001095 DATMCV010000000
VERSION     DATMCV010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794470"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..334)
                     /locus_tag="VNU03_25005"
                     /pseudo
     CDS             complement(194..334)
                     /locus_tag="VNU03_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            514..1878
                     /locus_tag="VNU03_25010"
     CDS             514..1878
                     /locus_tag="VNU03_25010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWP77805.1"
                     /translation="MSQEFDVVCLGGGVAGEAIAVGLQDSGLTLAVVERELVGGECPY
                     WGCIPSKTLLRSGETLSEAGRARVLAASRVEWAVDFPKVSKRALWMARDLDDSRPAAA
                     MEATGARLFRGEGKLTDLRTVAVGSEQVIARRAVVIANGSTAAIPAIPGLDTVPFWTN
                     RQAVIPKELPASLAVLGGGAIGVELGQAFARLGSKVTLIEAGPRFLGLEEPEAGAALR
                     PHLEADGITLMIRDPCVAVEKKSAAVALHLKSGTVVSADRLLVATGRRPNVEAWRGIG
                     IAQTERGWLKVDPATLEARAGVFGAGDVTGLGGFTHLAYYHGQVVARRLRGMDARADH
                     TAVPRVTFTDPEIASVGLSEAAAREKGIDVVVASADPAETARGYIHDFHGGALKLVGD
                     RTRRVLIGATLVTPRAGEILGELVLAIKLGTPLPTLADVIHPFPAFNRVLGASLAELA
                     AKVA"
     gene            1902..2546
                     /locus_tag="VNU03_25015"
     CDS             1902..2546
                     /locus_tag="VNU03_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP77806.1"
                     /translation="MSRVETKLEVVTIPVSDVDRAKRFYEGLGWRLDADFAKGDWRVV
                     QLTPPGSPCSIFIGKGLTTVAPGSVQGTVLIVDDIQAARAELVAHGADPSEVFHFDGT
                     LRVTGTQGRLPGPDPERRSYSSWVSFSDPDGNGWLLQEVTTRLPGRGLSSFDVATLTD
                     LLRETEQGHGQYEATAPKHHWSGWYAAYIVARQQGKTPEDAAKDAARHMESARG"
     gene            complement(2569..3474)
                     /locus_tag="VNU03_25020"
     CDS             complement(2569..3474)
                     /locus_tag="VNU03_25020"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HWP77807.1"
                     /translation="MATTPTVLDVDPGIDDALALLLAWGSPELDVLALTVVAGNAPLA
                     ATARNAARLVALRRPARLPQVALGAAAPLRRALVTATPGDHGADGLGDVPDWPPVTGP
                     PASPSAAETLVALARAHGERLALIALGPLTNLALALRLDAEAMRRIRRVVVMGGAVDV
                     PGNTATDAEFNIYVDPEAAGEVFDAGLRVDLVPLDATRQTRINGDQLHAALAGRRGPL
                     ATRIEAFTEQSFRLWGYMHLHDPLAVGLAVDESLARWQPARIAVGDGGQTRRAAGAAN
                     CRVAQVIDAERFLAMFFDRLCPVSP"
     gene            complement(3587..4303)
                     /locus_tag="VNU03_25025"
     CDS             complement(3587..4303)
                     /locus_tag="VNU03_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HWP77808.1"
                     /translation="METFERTPAPAFGGRGIAAAERATAFLRTVYGWMCAGLAITALV
                     AYSIASSPVLVQTLVTNHLLFLGLFVGQIALVFFLSARADRLASGIATLLFIAYAALT
                     GVTLSVLVLAYTGASVATTFVVTAGMFGALALYGSTTKRSLAGAGQFFFMGLIGLVLA
                     SIVGIFWHSAALQFLISVVGVIIFTGLTAWDAQRLKEMALALPEGQVGSYAIVGALSL
                     YLDFINLFLFLLRFLGDRRD"
     gene            complement(4350..>4971)
                     /locus_tag="VNU03_25030"
     CDS             complement(4350..>4971)
                     /locus_tag="VNU03_25030"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HWP77809.1"
                     /translation="IGFLGLGGLFTAHITGNLVVLSAHVVSGRTAPFAAMLAVPVFMA
                     VLGLTRLLAAGLEAIGLATLRPLLLLQFLLLGGFLGLCVAAGPRIDPHAPTAILAGML
                     GVSAMAVQNALLQISLTGAPATAVMTTNVARFMIDIGDVLLGRDSEAVARARNRAKHT
                     WPPIVGFTMGCGLGAAGEAAIGLWSLALPAGLALLACVLARTFPSP"
BASE COUNT          836 a         1669 c         1790 g          676 t
ORIGIN      
        1 tcaccatgtc ctgtcaggga cttggccggc atcacgcccg ccaggctgag tcagccggat
       61 ctggtcttgg agggcggtgc ggtggccgcc tgacgctcgc gctgcatctc ccgcacacgg
      121 agccgacggg cagcgcgtcg aatccgctgg actgcggtcc tggtcttcga gtgggtggcc
      181 attggcgtct cctctattcg atcacgtgat ccgcctgctg caagagcgac ggcgggatcg
      241 tcagaccgag gttgatcacg agctcgagct tcgtcggctg ctcgaccgga agggcagcgg
      301 gactggcgcc tcgcaggatt cggtcgacat aggtgccgat gaacacgcgc cgatccatcg
      361 ccgatctcat cccgggccag agtctagcaa agggatactg gtaccgcatg gtcgattgac
      421 ggcggcagtg ggtgtggcct agcatggcgc tcacccgggc cgcctctcac gacgcggcgc
      481 cgcattccat gatggggcac ggaggtagga gagttgtccc aggagtttga cgtcgtgtgt
      541 ctcggcggtg gcgtcgccgg agaagcgatc gcggtgggcc ttcaggacag cgggctgacg
      601 ctcgcggtgg tcgagcgaga gctcgtcggg ggcgagtgcc cgtactgggg ctgcatccct
      661 tccaagacct tgcttcgctc aggcgaaacg ttgagcgagg ccgggcgcgc tcgcgtcctg
      721 gcggcctcac gcgtggaatg ggccgtcgac tttcccaagg tttcgaaacg cgcgctgtgg
      781 atggcacgcg acctcgatga cagccggcct gccgccgcga tggaggcgac cggggcgcgg
      841 ctctttcggg gcgaagggaa actgacggac ctgcgtacgg tcgcggttgg aagcgagcag
      901 gtcatcgcgc gccgggccgt cgtgatcgcc aacggcagca ccgcggcgat tccagccatc
      961 cccggcttgg acaccgtccc gttctggacc aaccgccagg cggtgatccc gaaggagctg
     1021 ccggcttcgc tggcggtcct cggcgggggg gccattggcg tcgagctggg gcaggccttc
     1081 gcacggctcg gctccaaggt gacgctgatc gaagccggac cgaggttcct tggcctcgag
     1141 gagccagagg ccggggccgc gctccggcca cacctggagg cggacggcat cacgctgatg
     1201 atcagggacc cctgtgtcgc cgtcgaaaag aaatccgcgg ccgtcgccct ccacttgaag
     1261 tccggaacgg tggtctcggc cgaccgattg ctggtcgcga cgggccgccg ccccaacgtg
     1321 gaggcatggc gcgggatcgg gatcgcacag acggagcgcg gttggttgaa ggtggacccg
     1381 gcgacgctcg aagcgcgggc cggcgtcttt ggagccggtg acgtcacggg actcggcggc
     1441 ttcacccacc tcgcctacta ccacggtcag gtcgtcgcgc ggcgattgcg gggcatggac
     1501 gcgcgggccg accacaccgc cgtcccgcgc gtgacgttca ccgatccgga aatcgcttcg
     1561 gtcgggctgt cggaagcggc cgcgcgcgag aaaggcatcg acgtcgtcgt cgccagcgcc
     1621 gatccggccg agaccgcgcg aggctacatc cacgactttc acggaggcgc gctcaagctg
     1681 gtcggcgacc gcacccggcg ggtgctgatc ggcgcgacgc tcgtcacgcc gcgggccggc
     1741 gagatcctgg gcgagctcgt gctggcgatc aagctgggca ctccgctccc aacgctcgcc
     1801 gacgtcatcc acccgttccc agccttcaac cgcgtgctcg gggcgagcct cgccgagctc
     1861 gcggcgaagg tcgcctgaac gccaagaaag cggaggccac gatgagcagg gtcgaaacga
     1921 agctcgaggt cgtcaccatc ccggtgtccg acgtcgatcg cgccaaacgc ttctacgagg
     1981 gcctggggtg gcggctcgac gccgattttg ccaagggcga ctggcgtgta gtccagctga
     2041 cgccccccgg ctcaccgtgc tccatcttca tcgggaaggg tctcacgacc gtcgcgccgg
     2101 gctcggtgca gggaacggtc ctcatcgtcg acgacatcca ggccgcgcgc gccgagctcg
     2161 tcgcccacgg tgccgacccg agcgaggtct tccatttcga cggcacgctc cgtgtcaccg
     2221 ggacgcaggg acgcttgccg ggcccggacc cggagcggcg ctcctacagc tcgtgggtct
     2281 cgttcagcga tcctgacggc aacggctggc tgctgcagga ggtcaccacc cggcttcctg
     2341 gacgcgggct gagcagcttc gacgtcgcga cgctgaccga tctcctgcgc gaaaccgagc
     2401 aaggccacgg ccagtacgaa gcaaccgccc cgaagcacca ctggtcgggc tggtacgccg
     2461 cctacatcgt cgcgcgccag caggggaaga cccccgagga cgcggccaag gatgccgccc
     2521 gccacatgga gagtgcccgc gggtgacggc cggcggatcg attccgccct acggggacac
     2581 ggggcacagc cggtcgaaga acatcgcgag gaagcgctcc gcgtcgatga cctgcgccac
     2641 gcggcagttc gcagcgccgg ccgcgcgccg ggtctgaccg ccgtcgccca ccgcgatccg
     2701 cgccggttgc catcgcgcca ggctctcgtc gacggcaaga cccaccgcca gagggtcatg
     2761 caggtgcatg tagccccaga gacggaagct ttgctcggtg aacgcctcga tgcgggtggc
     2821 gagcggtccg cgccggccgg ccagcgcggc gtggagctgg tcgccgttga tcctggtctg
     2881 acgcgtggcg tccagcggca cgaggtccac ccgcaggccg gcgtcgaaga cctcgcccgc
     2941 ggcctccgga tcgacataga tattgaactc ggcgtccgtg gcggtattgc cgggcacgtc
     3001 taccgcgccg cccatcacga ccacccggcg gatcctccgc atcgcctcgg catcgagccg
     3061 aagagcgagc gccaggttgg tcagcgggcc cagcgcgatg agcgccaagc gctcgccgtg
     3121 ggcccgggcc agggccacga gcgtctcggc cgccgacggc gaggcgggcg ggccggtcac
     3181 gggcggccaa tccggcacat cgccgaggcc gtcggcgccg tgatcgcccg gagtggcggt
     3241 caccagcgcc ctgcgcagcg gggccgcggc gcccaaagcg acctggggca ggcgagccgg
     3301 ccggcgcagc gcgaccaggc gggcggcgtt gcgcgccgtg gcggcgagcg gcgcgttgcc
     3361 cgcgaccacc gtcagcgcca ggacgtcgag ctcgggcgag ccccaggcga gcagcaacgc
     3421 cagggcatcg tcgataccgg gatcgacatc gaggacggtc ggggtcgttg ccatgcgggg
     3481 attctaccag ccgagccgag cgaccgccgg cccttccaat cagacgacgt ccacctcgct
     3541 agccacgttg cgggaagtag ggccgacgct ggcggaggac gaacgatcag tcgcggcgat
     3601 cgccgaggaa gcgcaggagg aagaggaaga ggttgatgaa gtcgaggtag agggatagcg
     3661 cgcccacgat ggcgtaggag ccgacctggc cctcgggcag ggcgagcgcc atctccttca
     3721 gccgctgggc gtcccaggcg gtgagtccgg tgaagatgat cacgcccacc acggagatca
     3781 ggaactgcag ggctgcgctg tgccagaaga tccccacgat ggacgccagc acgagcccga
     3841 tcaatcccat gaagaagaac tggcccgcgc ccgccaggct ccgcttggtg gtcgagccgt
     3901 agagcgccag ggcgccgaac atgccggcgg tcacgacgaa cgtcgtcgcg accgaggctc
     3961 cggtgtaggc gagcaccagg accgacaggg tcacgccggt cagggcggca tacgcgatga
     4021 agagaagggt ggcgatgccg gaggcgagcc ggtcggcgcg ggccgagagg aagaacacca
     4081 gcgcgatctg tccgacgaac agaccaagga agagcaggtg attcgtgacc agggtctgta
     4141 cgagcacggg cgagctcgcg atcgagtagg ccacgagggc cgtgatcgcg agtccggcgc
     4201 acatccagcc gtacaccgtc cggaggaagg cggtggcgcg ctcggcggcc gcgatacccc
     4261 tccccccgaa tgccggagcg ggagttctct cgaaagtctc catgggatcc tccagttctc
     4321 tccagtgtgg aggagagcgg gacggatgtc tatggtgacg ggaaagttct cgcaagaaca
     4381 cacgcgagga gggcgaggcc ggcgggcagc gccagggacc acaggccaat tgcggcctca
     4441 cccgcggcac cgaggccgca gcccatggtg aagcccacga ttggcggcca ggtgtgcttc
     4501 gcccggttgc gcgccctggc gaccgcctcc gaatcgcgcc ccagcaagac gtcgccgata
     4561 tcgatcatga agcgggcgac gttggtcgtc atcaccgccg tggccggcgc cccggtcagt
     4621 gagatctgca gaagggcgtt ctgcaccgcc attgccgaca cgccgagcat gcccgcgagg
     4681 atcgcggtcg gcgcgtgagg gtcgatgcgc ggtcccgccg cgacgcagag cccgagaaag
     4741 ccgccgagca gcaggaactg cagcagcaac aagggtcgca gcgtggcgag cccgatcgct
     4801 tccaagccag ccgccagcag cctcgtcagg ccgagcaccg ccatgaacac cggaaccgcc
     4861 agcatcgccg cgaacggcgc ggtgcgaccg ctgacgacgt gcgcggacag gacgaccagg
     4921 ttcccggtga tgtgggcggt gaacagcccg ccgagcccaa ggaagccgat g
//
LOCUS       DATMCV010001096         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_661938, whole genome shotgun sequence.
ACCESSION   DATMCV010001096 DATMCV010000000
VERSION     DATMCV010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661938"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..2668)
                     /locus_tag="VNU03_25035"
     CDS             complement(188..2668)
                     /locus_tag="VNU03_25035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HWP77810.1"
                     /translation="MSASRPAVRLSTGSGGLEWPEVLALLAREARTPMGREEALAAIP
                     LADPDAVRRALGETAQARAALGQTGAPPWEGVVDVRPMLEAVRVPGSVAEAADLAAII
                     PLLEAAGRLRAYGRAIAPVAADLAAALTAFPSQKDLADLLARSIDADGQLRDDASPAL
                     RRVRGRIRDQRREIVKRLEAYFGAPGAEATFQERYVTVRHGRYVLPIRAEAKSRLRGI
                     VHDRSQSGATLFVEPEGMVEANNDLVQAAREEETEILRILAALTDAVREALPDLDALV
                     AGIGGLDLIFARGALAERMEAVEPGIAEAGDVSLPGARNPLLLAQAWRGAAEGEPSGS
                     IMQVIPMDIEIHADRPLLVITGPNAGGKTVALKTLGLLALMAQAGCHVPARAGARLPV
                     FSQVFAIVGDDQSVAENLSTFSAFVKQLRDVLERVDGRSLVLLDELGAGTDPDDGAAL
                     AQAVLEALAERGAVVAASTHLEPLKGFASTHPRARNASVEFDPERLAPTFRLVYDRPG
                     QSYALSIGARLGLPPALIERAHAHRSTQQRQLQELLARLDDRDRRDAERTAALERREA
                     ESAGLLARAQAELEAARATAREAVARAKAEAQRLVTEVRRHVNDEWDRLKRAEKSRPD
                     LERARKRLVETAQRVEQTAGAAAPSPALAGAATPGDRVEVSHLGLKGRVLAVDGETAT
                     VQAGAVTVKVPRQALTVVARGEAGLDAVAPGEGVMYPRPRMGTGRSAKAGSRGVVAVP
                     GKSGVPGELHLIGRTTDEARDLLEKYLDDAFLAGLPTVRIIHGKGTGALRRAVEDVLG
                     GHPLIVEHRPGAAAEGGGGATVATLAQG"
     gene            complement(2682..2840)
                     /locus_tag="VNU03_25040"
     CDS             complement(2682..2840)
                     /locus_tag="VNU03_25040"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77811.1"
                     /translation="MKIGVGRPGRPREDRDQVADHVLSPFLPEEHDAVAQACDEAADQ
                     VMKLVEGR"
BASE COUNT          329 a         1057 c         1085 g          376 t
ORIGIN      
        1 tagatgttgc gcttcgggtt caccgtgaac gacgcggtct tctcggtgtg gaaggggcag
       61 aggcccttcc agttctcgcc cgcccgcttg agcttcacca cctggccgac gatctcgagg
      121 atatcgacgc gagagcggat ctcgtcgagg tgctgctggg tgaatccgcc cggcatggcg
      181 tgaggcctca gccctgggcc agggtcgcca ccgtcgcgcc gccgccgccc tcggcggcgg
      241 cgcccggccg gtgctcgacg atcaggggat ggccgcccag gacgtcctcc accgcgcgcc
      301 ggagcgcacc ggtccccttg ccgtggatga tgcggacggt ggggagcccg gccaggaagg
      361 cgtcgtcgag gtatttctcg aggaggtcgc gtgcctcgtc ggtcgtccgg ccgatgaggt
      421 gcagctcgcc ggggaccccg ctcttgcccg gcaccgccac cacccctcga gatccggcct
      481 tcgcgctccg ccccgtcccc atccgggggc gagggtacat gactccttcg ccgggcgcca
      541 ccgcgtcgag gccggcctcg ccacgggcca ccaccgtgag cgcctgccgc ggcaccttca
      601 cggtgaccgc gccggcctgc acggtggcgg tctccccgtc gaccgcgagc acccggccct
      661 tgagaccgag gtgcgagacc tcgacgcggt ctcccggcgt ggcggcgccc gcgagcgcgg
      721 gtgaaggcgc ggccgcgccc gcggtctgct ccacgcgctg tgccgtctcg accaggcgtt
      781 tgcgcgcgcg ctcgaggtcg gggcggctct tctcggcgcg cttcaggcga tcccactcgt
      841 cgttcacgtg tcggcgcacc tcggtgacga gccgctgggc ctcggccttc gcgcgggcca
      901 ccgcctcccg cgcggtggcc cgcgccgcct ccagctcggc ctgggcccgc gcgagcaggc
      961 ccgcgctctc cgcctcgcgg cgctcgagcg cggcggtgcg ctcggcgtcc cgccggtcgc
     1021 ggtcgtcgag gcgggcgagc agctcctgga gctgccgctg ctgggtcgag cggtgcgcgt
     1081 gggcgcgctc gatcagggcg ggcggcaggc cgagccgcgc accgatcgac agggcatagc
     1141 tctggccggg ccggtcgtag acgagccgga aggtgggcgc gaggcgctcc gggtcgaact
     1201 cgaccgaggc gttgcgggcc cgcgggtggg tggaggcgaa gcccttcagc ggctcgagat
     1261 gcgtcgaggc cgccaccacc gcgccgcgct ccgcgagcgc ctcgaggacg gcctgggcca
     1321 gagcggcccc gtcgtcgggg tcggtgcccg cgcccagctc gtcgagcagc acgagcgagc
     1381 ggccgtcgac gcgctccagc acgtcgcgga gctgcttgac gaacgccgag aacgtcgaga
     1441 ggttctccgc cacgctctgg tcgtcgccca cgatggcgaa gacttgggag aagacgggca
     1501 ggcgcgcccc cgcgcgcgcg ggcacgtggc agcccgcctg cgccatcagg gcgagcaggc
     1561 ccagggtctt gagcgcgacc gtcttgccgc ccgcgttggg gccggtgatg acgagcagcg
     1621 ggcgatccgc gtggatctcg atgtccatcg gaatgacctg cattattgat ccgctcggct
     1681 cgccttcggc tgcacctcgc caagcttgcg cgaggagcag cgggttgcgg gcgccgggga
     1741 gggagacgtc gcccgcctcg gcgatcccgg gctcgaccgc ttccatgcgc tcggcgaggg
     1801 cgccgcgggc gaagatcagg tcgagcccgc cgatgccggc cacgagggcg tccaggtccg
     1861 gcagcgcctc tcgcaccgcg tcggtgaggg cggccaggat gcggaggatc tcggtctcct
     1921 cctcgcgcgc ggcctgcacg aggtcgttgt tggcctcgac catcccctcc ggctcgacga
     1981 agagcgtggc cccgctctgg gagcggtcgt ggacgatgcc gcgcaggcga ctcttggcct
     2041 cggcgcggat cggcagcacg tagcggccgt gacggacggt gacatagcgc tcctggaagg
     2101 tggcctccgc gccgggcgcg ccgaagtagg cctcgagccg cttgacgatc tcgcgccgct
     2161 ggtcgcggat gcgcccgcgc acccggcgga gcgcggggga cgcgtcgtcg cgcagctggc
     2221 cgtcggcgtc gatggagcgg gcgagcagat cggccaggtc cttctgcgac gggaaggcgg
     2281 tgagggcggc ggccaggtcg gcggcgaccg gggcgatcgc gcggccgtag gcgcgcagcc
     2341 gtcccgccgc ctccagcagc gggatgatcg cggccaggtc ggccgcctcc gccaccgagc
     2401 ccggcacgcg cacggcctcg agcatcggcc gcacgtcgac cacgccctcc cacgggggcg
     2461 cgccggtctg gcccagggcg gcccgcgcct gggcggtctc gccgagcgcg cggcggaccg
     2521 cgtccggatc ggcgagcggg atggcggcca gggcctcctc gcggcccatc ggggtgcggg
     2581 cctcacgcgc gagcagggcc agcacctcgg gccattcgag cccgccgctg ccggtggaga
     2641 gccgcaccgc gggccgggac gcgctcatgc ggccgccgcg ctcagcggcc ctccacgagc
     2701 ttcatcacct gatcggccgc ctcgtcgcag gcctgggcca ccgcgtcgtg ctcctccggc
     2761 aagaacggcg agagcacgtg gtcggcgacc tggtcgcgat cctcgcgcgg ccggccggga
     2821 cggcccacgc cgatcttcac gcgccgg
//
LOCUS       DATMCV010001097         6130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_129331, whole genome shotgun sequence.
ACCESSION   DATMCV010001097 DATMCV010000000
VERSION     DATMCV010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129331"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VNU03_25045"
     CDS             <1..273
                     /locus_tag="VNU03_25045"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HWP77812.1"
                     /translation="NRRFADITMGFFKKHHIQVVGFWTNELGGASDQLIYILAYDSLA
                     DREKKWGGFFADQDRLAKFAETEKNGPLVRRLTAQILKPTAYSPMQ"
     gene            274..453
                     /locus_tag="VNU03_25050"
     CDS             274..453
                     /locus_tag="VNU03_25050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77813.1"
                     /translation="MRDVAALLLAVLLAGCGPVAFGSAYSEEELARSCAQRGGWWRPD
                     ELRGGFCEFEAPGFI"
     gene            complement(468..1502)
                     /locus_tag="VNU03_25055"
     CDS             complement(468..1502)
                     /locus_tag="VNU03_25055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWP77814.1"
                     /translation="MTEPFVTLPEMHRAARARLPRTSYNFGAGGTETETTLRRNRRSL
                     RRLAIRQDILVDVRQIDLSTSLLGVPLSWPVVIAPMGGLVLFHPEGDCEMARGAAKAD
                     TLQFLSGATGWSVEAVAAASPGLKMFQLYHMGDRAWVADLLARVEASGYLCVCLTVDV
                     QVYSRRERDILARFTPREAMSKAPNPRPPDSEYPARLTWADVEWLRKTTRLPIGLKGI
                     MTPRDAKRAVEAGVELIWVSNHGGRQLDSTQASIDALPAVADVVGGAASIIVDGGFAR
                     GTDVIKGMAWGANAVALGRTALWGLAADGAAGVACAMDILRRELRTSMALAGQTSVRG
                     LTRDAVFRVD"
     gene            1557..2111
                     /locus_tag="VNU03_25060"
     CDS             1557..2111
                     /locus_tag="VNU03_25060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77815.1"
                     /translation="MPLKRVPYRSLRTLLRRELITREDPGTAALIRRLAHVKAAGRFT
                     RGEFLAMCRWKSPRSRLHYERSGPATIRRVSTAALAARDEQARIAHLVALPGVSVATA
                     SAILTLIDPARYGVLDIRCWQLLFSLRSVAGNRRGRAFTLAQWEQYLTRLRGHARALG
                     VSARTIEYTLFHCHRRLQRGRLYD"
     gene            complement(2116..2550)
                     /locus_tag="VNU03_25065"
     CDS             complement(2116..2550)
                     /locus_tag="VNU03_25065"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWP77816.1"
                     /translation="MAKRILVPLDGSESAEAAATLAADLARSSGGHVRLLQVRPVPEH
                     RVSLAGRVVAFADQEMERIAAEGRDYLETVETRLGAVPTEAVVRFGEPAQEILVEAEA
                     FDADLIVLTAERRGWLARFTGGGLGERLLQASPVPVALVRAA"
     gene            2682..3032
                     /locus_tag="VNU03_25070"
     CDS             2682..3032
                     /locus_tag="VNU03_25070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77817.1"
                     /translation="MLEIVVAAVIGLILVVGPLGWRVQRDRAESRALATRAYVQSLID
                     RRLGGESFVSVQVTPRSLWSQGQVVVSVPGGWEWMLEDVWPVLLARVPRGFELVVRRG
                     EEAPAPARPLRRVA"
     gene            3133..4266
                     /locus_tag="VNU03_25075"
     CDS             3133..4266
                     /locus_tag="VNU03_25075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77818.1"
                     /translation="MHVGMAAVFQNPGKAQSDRDVYLNELRLAELAEPLGFQSVWGVE
                     HHFTDYTMCPDVLQFLSYMAGRTRHAQLGSMVVVLPWHDPMRVAEEVSMLDNLSGGRL
                     ILGLGRGAGKVEFDGFRLSMDESRPRFVESAEMLLRGLETGYCEYDGAYVKQPRAAIR
                     PAPFKSFRGRTYAAAVSPESVQIMAELGVGILIIPQKPWKEVARELHAYRDVYRRVNG
                     VDAPPPISAGWTFCDASADRAHEMARRYIGGYYQTVLDHYQFQGDHLAHTRGYEYYGK
                     MAEKIQQYGTDTVVDYFTNLQVWGTPEQCYEKILDIHARTGNSHYVGVFGYAGMPYDE
                     AERNLRFFAKEVMPALQKLDTAAADAWQAPAAAEKPGVGLLGS"
     gene            complement(4291..5076)
                     /locus_tag="VNU03_25080"
     CDS             complement(4291..5076)
                     /locus_tag="VNU03_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP77819.1"
                     /translation="MPDPAARRFEGKVVLITGAAGGIGRATAVRFATEGARLGLVDRA
                     RDGLRETLAAVEKAGGAGVTVEADVTRSADVARYAAAVAERWGGVDCFFNNAGILGDV
                     RALVDYPEETFDRVIAVNVKGVWLGIKTMAPLLRARGGGVIVNTASIAGLRGSRNLVA
                     YTASKHAVVGLTRTAALELAPAGIRVNAVCPSPIDTAMVQALETGASPSNPAAFHERM
                     AGTIPLRRYGQPEEVAALVAFLCSADAAYITGGIYTVDGGSMA"
     gene            complement(5076..5423)
                     /locus_tag="VNU03_25085"
     CDS             complement(5076..5423)
                     /locus_tag="VNU03_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP77820.1"
                     /translation="MSIKRHGVGPRLSQAVVHGNTVYLAGIIAGDPSADAKGQTEQIL
                     KKIDEHLAAVGSHKSKLLSATIYVANMGIFGEMNSAWDAWIDPANTPARATVEARLAA
                     PKYLVEIMAIAAI"
     gene            5537..5893
                     /locus_tag="VNU03_25090"
     CDS             5537..5893
                     /locus_tag="VNU03_25090"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HWP77821.1"
                     /translation="MRTLGRVAIVAGLLLVLSGCEREEKAVREDLPLARTTKARTDAQ
                     TIATAVNTYRVTCGALPESIEALVTQTTVGGAPCGPVLGSIPAPPTGWSPYVYARQGD
                     SFTITSSGNGQTVSAP"
     gene            5902..>6130
                     /locus_tag="VNU03_25095"
     CDS             5902..>6130
                     /locus_tag="VNU03_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77822.1"
                     /translation="MIVVTRRRFVAGVVAALGAGLAAPRPGAAQSASAGPPEPQLLED
                     LVAANRILVDQGVLDGYGHVSARHDRDAGRFL"
BASE COUNT          858 a         2190 c         2185 g          897 t
ORIGIN      
        1 aaccgacgct tcgccgacat caccatgggt ttcttcaaga agcaccacat ccaggtggtc
       61 ggcttctgga ccaatgagct gggcggcgcc tcggaccagc tcatctacat cctcgcgtac
      121 gacagcctgg ccgatcgcga gaagaagtgg ggcggcttct tcgcggatca ggatcggctg
      181 gccaagttcg ccgagaccga gaagaacggc ccgctggtgc gccggctcac cgcgcagatc
      241 ctcaagccca ccgcgtactc gccgatgcag taggtgcgag acgtcgccgc gctcctgctg
      301 gccgtgctgc tcgcggggtg cgggccggtc gccttcgggt ctgcgtactc ggaggaggag
      361 ctggcccgga gctgcgcgca gcgcggcggc tggtggcgtc ccgacgagct ccgcggcggc
      421 ttctgcgagt tcgaagcgcc ggggttcatc tgacccgcgg cgccggctca atcgacccgg
      481 aagaccgcgt cgcgggtgag cccgcgcacg ctcgtttgcc cggccagcgc catggaggtg
      541 cgcagctcgc ggcggaggat gtccatcgcg caggccaccc cggccgcgcc gtcggccgcg
      601 agaccccaga gcgcggtgcg tcccaacgcg accgcgttcg ccccccacgc catgcccttg
      661 atcacgtcgg tgccccgggc gaagccgccg tcgacgatga tgctggccgc gccgccgacc
      721 acgtcggcca ccgcgggcag cgcgtcgatg ctggcctggg tggaatcgag ctgacgcccg
      781 ccgtgattgg acacccagat cagctcgacg cccgcctcga ccgcacgctt ggcgtcgcgg
      841 ggagtcatga tgcccttgag tccgatgggc agtcgcgtgg tcttgcgcag ccactcgacg
      901 tcggcccagg tgaggcgcgc cgggtattcc gagtcggggg gccggggatt gggcgccttc
      961 gacatcgcct cgcgcggggt gaagcgggcg aggatgtcgc gctcgcggcg gctgtagacc
     1021 tggacgtcga cggtgaggca cacgcagaga tagccggagg cctccacccg cgcgagcaga
     1081 tccgcgaccc acgcgcgatc gcccatgtgg tagagctgga acatcttgag cccgggactg
     1141 gctgccgcca ccgcctccac cgaccacccg gtggctccgc tcaggaactg cagggtgtcg
     1201 gccttggccg ccccgcgggc catctcgcag tcgccctcgg ggtggaagag cacgagcccg
     1261 cccatcggcg cgatcaccac cggccacgag agcggcaccc cgagcagcga ggtggagaga
     1321 tcgatctggc gcacgtcgac caggatgtcc tggcggatgg cgaggcgccg cagcgagcgg
     1381 cggttccggc ggagcgtggt ctcggtctcg gtgccgcccg cgccgaagtt gtacgaggtg
     1441 cggggcaggc gcgcgcgggc cgcgcggtgc atctcgggaa gcgtcacgaa gggctcggtc
     1501 atggcgggta cgataatgcc tgccgcacgt gagcgcaacg gcgggaggcg ccggcgatgc
     1561 cgctgaagcg ggtgccctac cggagcctgc ggaccctgct gcgacgcgag ctgatcacgc
     1621 gggaagaccc gggcaccgcc gcgctcatcc ggcgcctcgc ccacgtgaag gcggcggggc
     1681 ggttcacgcg gggcgagttc ctcgcgatgt gccggtggaa gagcccgcgg tcccgcctcc
     1741 actacgagcg gagcgggccc gcgacgatcc gccgggtctc caccgccgcg ctggccgcgc
     1801 gcgacgagca ggcgcgcatc gcccacctcg tcgcgctgcc cggggtgagc gtggccaccg
     1861 cgtccgcgat cctcaccctc atcgatccgg cgcgctacgg cgtactcgac atccgctgct
     1921 ggcagctcct cttcagcctc cgctccgtcg ccggcaatcg gcgcgggcgg gccttcacgc
     1981 tcgcgcagtg ggagcagtac ctgacgcgac tgcgcggaca cgcccgggct ctgggcgtat
     2041 ccgcgcggac catcgagtac acgctgttcc actgccaccg gcggctacag cgcggccggc
     2101 tctacgactg agacctcagg cggcgcggac gagcgcgacc gggaccggag aagcctgcag
     2161 gagccgctcg ccgaggccgc cacccgtgaa gcgggccagc cagccccgcc gctcggcggt
     2221 cagcacgatg agatcggcgt cgaaggcttc cgcctccacc aggatctcct gggccggctc
     2281 cccgaagcga accaccgcct cggtgggcac cgccccgagc cgcgtctcca ccgtctcgag
     2341 atagtcgcgg ccctccgcgg cgatccgctc catctcctga tcggcgaacg ccaccacgcg
     2401 cccggccaag ctcacccggt gctccggcac cggacgaacc tgcaacagcc ggacgtgccc
     2461 gccgctgctc cgggccaggt cggccgccag ggtcgccgcg gcctccgcgg attcactacc
     2521 atccaaagga acaagaattc gctttgccat gatcccttcc tccttcctca atcgtctttt
     2581 ggatgccgcg gtgcgcccaa gagattccac ctttccctgt cgggatctag cggaatccct
     2641 tggccctccc ccggcatctc aatgcccgga aggagatagc catgctcgag atcgtggtgg
     2701 cggcggtgat cggattgatc ctggtggtag gccccctggg ctggcgggtg cagcgggacc
     2761 gcgccgagtc gcgcgcactt gcgacgcgcg cctacgtgca gtccctcatc gaccgcaggc
     2821 tgggcggtga atccttcgtg tcggtgcagg tgacgccgcg ctcgctctgg tcgcagggcc
     2881 aggtggtggt gtcggtgccc ggcggctggg agtggatgct ggaggatgtc tggccggtgc
     2941 tgctggcccg cgtaccccgg ggcttcgagc tcgtggtgcg gcggggcgag gaagccccgg
     3001 ctccggcgcg tccgctccgt cgcgtcgcct gatctaggct tcccggcggg acccctctag
     3061 gcttcccggc gggaccccgg cttgcccggc gggttccccc tcgcgtacca tcccggcgag
     3121 ggggaaccca ccatgcacgt cgggatggcc gcggtcttcc agaacccggg caaggcgcag
     3181 agcgatcggg acgtctacct gaacgagctc cgtctcgcgg agctggccga gccgctcggg
     3241 ttccagtcgg tgtggggcgt ggagcatcac ttcaccgact acacgatgtg ccccgacgtc
     3301 ctgcagttcc tctcctacat ggcggggcgc acccgtcacg cgcagctcgg ctccatggtc
     3361 gtcgtgctcc cgtggcacga cccgatgcgg gtggccgagg aagtctcgat gctcgacaac
     3421 ctgtcgggcg gccggctcat cctcggactg ggccgcggcg cgggcaaggt ggagttcgac
     3481 gggttccggc tgtcgatgga cgagtcgcgc ccgcgcttcg tggaatcggc tgagatgctg
     3541 ctgcgcgggc tcgagaccgg ctactgcgag tacgacgggg cctacgtcaa gcagccgcgc
     3601 gcggccatcc ggcccgcgcc gttcaagagc ttccgcgggc gcacctacgc ggcggcggtc
     3661 tcgccggagt cggtgcagat catggccgag ctgggggtcg gcatcctgat catcccccag
     3721 aagccgtgga aggaggtggc gcgcgagctt cacgcgtatc gcgacgtcta ccgccgggtc
     3781 aacggagtgg acgcgccgcc cccgatctcg gcgggctgga ccttctgcga cgcgagcgcc
     3841 gaccgcgccc acgagatggc gcggcgctac atcggcggct attaccagac cgtgctcgac
     3901 cactaccagt tccagggcga ccacctcgcc cacaccaggg gctacgagta ctacggcaag
     3961 atggcggaga agatccagca gtacggcacc gacaccgtgg tcgactactt cacgaacctc
     4021 caggtgtggg gcacgcccga gcagtgctac gagaagatcc tcgacatcca cgcgcgcacc
     4081 ggcaacagcc actacgtcgg cgtcttcggc tacgcgggca tgccgtacga cgaggcggag
     4141 cgcaacctgc gcttcttcgc caaggaagtg atgccggcgc tccagaagct cgacaccgcc
     4201 gcggcggacg cctggcaggc gccggccgcc gccgagaagc cgggcgtcgg tctcctcggc
     4261 tcgtagccga cggcaggccg agccgggcgg ctaggccatc gagccgccgt ccacggtgta
     4321 gatcccgccc gtgatgtagg ccgcgtccgc gctacagagg aacgccacca gggccgcgac
     4381 ctcttccggc tggccgtagc gccggagcgg gatggtgccg gccatacgct cgtggaacgc
     4441 ggccggattg ctggggctcg ccccggtctc gagggcctgg accatggcgg tgtcgatcgg
     4501 gctcgggcag accgcgttga cccggatgcc ggcgggggcc agctcgagcg cggcggtgcg
     4561 ggtgagcccg accaccgcgt gcttgctcgc ggtgtaggcc accaggttgc gggatccgcg
     4621 cagcccggcg atggaggccg tgttcacgat gacgcccccg ccgcgggccc ggagcagcgg
     4681 ggccatcgtc ttgatgccga gccagacgcc cttcacgttg accgcgatga cccggtcgaa
     4741 ggtctcctcg gggtagtcca ccagggcccg gacgtcgccc aggatgcccg cgttgttgaa
     4801 gaagcagtcg acgccgcccc agcgctcggc caccgcggcg gcgtagcgcg ccacgtccgc
     4861 cgaccgggtg acgtcggcct cgaccgtcac ccccgcgccg cccgccttct cgaccgcggc
     4921 cagggtctcg cggagcccgt cgcgcgcgcg gtcgacgagc ccgaggcgcg cgccctccgt
     4981 cgcgaagcgc accgcggtcg cgcgcccgat gccgccggcg gccccggtga tcagcaccac
     5041 cttgccctcg aatcgccggg ccgccggatc gggcatcaga tcgccgcgat cgccatgatc
     5101 tcgacgaggt acttgggggc ggccagccgg gcctcgacgg tggcccgggc cggggtgttg
     5161 gccgggtcga tccacgcgtc ccacgccgag ttcatctcgc cgaagatccc catgttggcg
     5221 acgtagatgg tggccgagag cagcttggac ttgtggctgc caaccgcggc gaggtgctcg
     5281 tcgatcttct tgaggatctg ctcggtctgg cccttggcgt ccgcggaggg atctcccgcg
     5341 atgatgccgg ccaggtacac cgtgttgccg tgaaccaccg cctggctgag ccgggggccg
     5401 acgccgtgtc gcttgatgct catcgtgatc tcctttgagt gggggtcgcc gcttggccgg
     5461 aagcgcccga gcggcgtatg attctgtcac aactcgacgg ccgcgtcgga ttttctcgag
     5521 cggaaaggac gggcgcgtga ggactctcgg ccgggtggcg atcgtggcgg ggctgctgct
     5581 tgtgctctcc gggtgcgagc gggaggagaa ggcggtgcgc gaggatctgc cgctggcgcg
     5641 caccaccaag gcgcgcaccg acgcccagac catcgcgacc gcggtgaaca cctatcgggt
     5701 tacctgcggc gccctgcccg agtcgatcga ggccctcgtg acccagacca cggtgggagg
     5761 cgcgccgtgc ggccccgtgc tcggctccat ccccgcgccg cccaccgggt ggtctccgta
     5821 cgtgtacgcg cggcagggtg actccttcac catcacgtcg agcggaaacg gccagaccgt
     5881 ttccgcgccc taggagaccc catgatcgtg gtgacccgtc gtcgcttcgt ggccggcgtg
     5941 gtggcggcgc tcggcgcggg gctcgccgcg ccgcggccgg gcgccgcgca gtcggcctcc
     6001 gcgggcccgc cggaaccgca gctgctcgag gacctggtcg cggccaaccg catcctggtc
     6061 gatcagggcg tgctggacgg ctacggccac gtgagcgcgc ggcacgaccg ggacgcgggc
     6121 cgcttcctga
//
LOCUS       DATMCV010001098         7720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_260966, whole genome shotgun sequence.
ACCESSION   DATMCV010001098 DATMCV010000000
VERSION     DATMCV010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260966"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..950)
                     /locus_tag="VNU03_25100"
     CDS             complement(21..950)
                     /locus_tag="VNU03_25100"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWP77823.1"
                     /translation="MTIDEIDTFVSIARRGGFARAAESLHRSQPAISRRIEMLEQQLG
                     TPLFERVRGGVILTEAGRTLLPYAEAVLAGLKDGAEAVRGLARGERGAVSVALVGTLA
                     STRFAGMLRQFGQRHPSVKLELRTANSEEVSDLVRRGEATLGLRYFTDTDPGLVSEQV
                     TEEALVLVCSPDHRLAGRRVRDPGALAGERWVAFPPRRRRREPFATALEQRLAAAGIE
                     AAEIVRIDSLTAQKRLVEAGFGIALLIESSVQEELRLGSLEIIHAPALQGGVPVTLVR
                     RRNGYLSGAAQSLVAVIKAGTATRARSRRAAGR"
     gene            1045..1407
                     /locus_tag="VNU03_25105"
     CDS             1045..1407
                     /locus_tag="VNU03_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWP77824.1"
                     /translation="MDIERHLIQDSENFKPIISRVVAQGDRVYVCGITGDPVGDVTAQ
                     TRQALERIDDLLARAGTDKSRLLTAQVWLSDMALFRTHNLAWNAWVDREHPPVRACVQ
                     AELTSPGLLVEIMVTAAR"
     gene            1440..2255
                     /locus_tag="VNU03_25110"
     CDS             1440..2255
                     /locus_tag="VNU03_25110"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP77825.1"
                     /translation="MLKTGADGGIVLAHATAVRAVAAWTLAPAPRDPAHHVEAAHAQA
                     RRHLARLTVHDPGREIPAVMTDELFSRLTDAHPAPLTAPAGVGRYTYTPRKALRRVLD
                     HALDHLNQIDQWIAWQRHGTVPTPTDGWVPSTVTLADDRLPLTAADLDAWLWRVDQAV
                     RLVAQRAAGLSEAELDWLPPDGGWPLRRVLHHLARSERLYAAALDEALLDDEPRARYR
                     EACRRLDDELRAVAGRGHDPSVVYAGLYGLLQSPEEVVDQVLAFEGELLAGVR"
     gene            complement(2272..2901)
                     /locus_tag="VNU03_25115"
     CDS             complement(2272..2901)
                     /locus_tag="VNU03_25115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77826.1"
                     /translation="MAFSRGFRTVTMALLVGGLAGCAAEPRQPASPAQRFTLLEHVQV
                     AGACILDNGGVSARYAGFVERWRDVKRGRDLLHGSVSTIDRFVDPGRSGHRPPLSIQF
                     GEIDVPPLSAGQLVLTGTSEGSQPSRATCTLDVTAREQPARTEGRLLASLLGREPVSF
                     PPRSVLSGAALLIGLVAGVRLIRHQVRRTSASWLLGLALLLQLVSLLLG"
     gene            2996..3583
                     /locus_tag="VNU03_25120"
     CDS             2996..3583
                     /locus_tag="VNU03_25120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77827.1"
                     /translation="MGRKGWGRLGAGLAAALGLAGCATGSGAYTEPEQARIDAYERAA
                     EQILQSRGMRGPAPAVRVGTDPSLAGAGRPAGYYTDQGRRIVVNRAAVADDYIAQAVL
                     SQELAHYVLGHADNACRERRLECEIEARIASVELLMTGWGLEYSDAIRLQYAYLKSVV
                     LAAQRGEVPATPGKSDPCRELQEFATRFKAQASCE"
     gene            3740..4504
                     /locus_tag="VNU03_25125"
     CDS             3740..4504
                     /locus_tag="VNU03_25125"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HWP77828.1"
                     /translation="MKIKVLGSFGSEGLGQRPSAFLVDDRVLVDAGTVGGALSVAEQI
                     EIQFALISHAHLDHTAGLAFLTDTLAMVAADRRVTACSIAPVIEALRTHAFNDALWPN
                     FTNIPSAAQSVLGFRTLPEEAESRVGELWVTPILVEHTVPTTGFVIHDGETGFVFSGD
                     TGPTKRIWQIAREMRGLKALIVETAFPNRLAALAKASGHLTPEMLGQEIAKMPADLPI
                     WVYHIKPQLYQETAEELAKIDPSRIHILEQGKTYTL"
     gene            complement(4501..5499)
                     /locus_tag="VNU03_25130"
     CDS             complement(4501..5499)
                     /locus_tag="VNU03_25130"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWP77829.1"
                     /translation="MAAPPARLELALGPTPILKLDRLSRRLGVELHVKRDDLTGLLES
                     GNKVRKLEFLVGEALQQGADTLVACGALQSNGCRAVAAVAARLGLRAVLLVRGEPPVA
                     YDGNLLLGRLLGAEVRYCAEADFARAGEIMETMATEIRARGGRPYLIPESGANEVGAL
                     GYLECAVELSEQINHGAPRFDAVVISAFTGGSQAGLLLGKRLAGLPGEIVGVPIARPA
                     ERVREHVVHTMGAAIRRWGFSIDVPKTVHLLDGDPGGDGLGATDADLAVLVELAREEG
                     VLLDPVYTARGFRGLVETLSRDPKALGQRVCFIHTGGLFSLFPYRERLSRLIDGGD"
     gene            complement(5507..5818)
                     /locus_tag="VNU03_25135"
     CDS             complement(5507..5818)
                     /locus_tag="VNU03_25135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77830.1"
                     /translation="MATNDRVLLTVLLHHDQSKPLDEIMAHLKKTGFYRDFPPEGSEL
                     VSWVVAMSFGFIIQLRVEPDKVRALNRYMEQKAWGAFRYQVFPAYDFAPIAANLKTEH
                     A"
     gene            6361..6558
                     /gene="rpsU"
                     /locus_tag="VNU03_25140"
     CDS             6361..6558
                     /gene="rpsU"
                     /locus_tag="VNU03_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012754552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWP77831.1"
                     /translation="MRIEVHDNQVESALKTMKKLMLKDGLFKEMKKRAYYEKPSVKRK
                     RKQAEARKKRRKAMKRAEADD"
     gene            6599..>7720
                     /locus_tag="VNU03_25145"
     CDS             6599..>7720
                     /locus_tag="VNU03_25145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77832.1"
                     /translation="MTPPRESLASQRLWLAFATMLLVAGITNTFPVFLPALLAEFGGS
                     RAATASTASLLWIGGALLSPVAGYLVARWNPRRLVCLGLAIVAGGMLLGSLAPTLPLF
                     LAAMGIGGGIGLGLTGMTTHAALIADAYVRRRGLATGIAFGGSMAAYALAPPAQWIIT
                     HWGWRPAFWCYAAAILALIPWAWRAHPTHLASIRNGGTGSSESAAVTIASIVRSAGFW
                     SLFVMFTTPPLFGYLATTQHALYFVERGFSAGQASVLLAIGGLLAGAGRALAGLTADR
                     LGGPTAGFLSFSCSLVGMGCLIGMEFRPVWLLAAGYVFFLFLPLGSRASIVTVLLGRI
                     APPAQYGVIFGLLGIGNNLGAALGPWLSGILFDRTGSYLV"
BASE COUNT         1118 a         2846 c         2654 g         1102 t
ORIGIN      
        1 gcgatcgatc gccatcctgg tcaccggccg gccgcccgtc ggctgcgggc gcgcgtcgcg
       61 gtccccgcct tgatcaccgc gacgaggctc tgcgcggccc cgctcagata cccgttgcgg
      121 cgccgcacca gggtcaccgg cacgccaccc tgcagcgcgg gcgcgtggat gatctcgagc
      181 gagcccaggc gcagctcctc ctgcacgctg ctctcgatga ggagcgcgat cccgaacccc
      241 gcctcgacca gccgcttctg cgcggtgagg ctgtcgatgc ggacgatctc cgccgcctcg
      301 atgccggccg cggcgagccg ctgctcgagc gcggtggcga agggctcgcg ccgccggcgc
      361 cggggaggaa aggccaccca gcgctcgccc gcgagggcgc cggggtcgcg tacccggcga
      421 ccggcgaggc ggtgatctgg cgagcagacg agcacgaggg cctcctcggt gacctgctcc
      481 gagacgagcc ccggatccgt gtcggtgaag taccggagcc ccagggtggc ctcgccgcgc
      541 cgcaccaggt cgctgacctc ctcgctgttc gcggtccgca gctcgagctt caccgacgga
      601 tgacgctggc cgaactggcg cagcatgccc gcgaagcggg tgctggcgag cgtccccacc
      661 agcgccaccg agaccgcgcc ccgctcgccg cgggcgagtc cccgcaccgc ctcggcgccg
      721 tccttcaggc cggccaggac cgcctcggcg tagggcagca gggtgcggcc ggcctcggtc
      781 aggatcacgc cgccgcgcac ccgctcgaag agcggggtgc cgagctgctg ctcgagcatc
      841 tcgatacgcc ggctgatcgc gggctgggag cggtgcaggc tctccgcggc gcgggcgaag
      901 ccgccgcggc gggcgatcga gacgaaggtg tcgatctcgt cgatggtcac cgcgatcact
      961 atagctgatg gccagcctca gttatatgca ttcgacggat ggcagccggc gcggcatcct
     1021 gtcgtgcgaa aaggagatat ggtcatggac atcgagcgcc acctcatcca ggacagcgag
     1081 aacttcaagc cgatcatcag ccgcgtcgtc gcgcagggcg accgggtata cgtctgcggg
     1141 atcaccggcg atccggtcgg cgacgtcacc gcccagaccc gccaggccct ggagcggatc
     1201 gacgacctcc tggcccgggc cggcaccgac aagtcgcggc tgctgaccgc gcaggtgtgg
     1261 ctctccgaca tggccctctt ccggacccac aacctcgcct ggaacgcgtg ggtggatcgc
     1321 gagcacccgc cggtgcgcgc ctgcgtccag gccgagctga ccagcccggg attgctcgtg
     1381 gagatcatgg tgacggccgc ccgatgagta cgcccgccct cgacgcgtcg agagaccgcg
     1441 tgctgaagac gggcgccgac ggcggcatcg tcctcgcgca cgccaccgcg gtgcgggcgg
     1501 tcgcggcctg gaccctggcg cccgcgccgc gggacccggc ccaccacgtc gaggcggccc
     1561 acgcgcaggc gcgccgtcac ctcgcccggc tcaccgtcca cgatccgggc cgcgagatcc
     1621 cggcggtcat gaccgacgag ctgttctcgc ggctcaccga cgcccatccg gctccgctga
     1681 ccgcgccggc cggggtcggc cgctatacct acaccccgcg caaggccctc cggcgggtgc
     1741 tcgaccatgc actggaccac ctcaaccaga ttgaccagtg gatcgcgtgg cagcggcacg
     1801 gcaccgtccc gacaccgacc gatggctggg tgccgtcgac ggtgaccctg gcggacgatc
     1861 ggctgccgct gaccgccgcc gatctcgacg catggctctg gcgggtcgac caggcggtcc
     1921 ggctcgtcgc gcagcgggcg gccgggctca gcgaggccga gctggattgg ctgccgcccg
     1981 acgggggctg gccgctccgc cgcgtgctcc accacctggc ccggtcggag cggctctacg
     2041 cggccgccct cgacgaggcc ctgctcgacg acgagccccg agcgcgctac cgcgaggcct
     2101 gtcggcgcct cgacgacgag cttcgcgcgg tggcggggcg tggccacgat ccgtccgtcg
     2161 tctacgccgg gctctacgga cttctacagt cgccggagga ggtggtcgac caggtcctgg
     2221 ccttcgaggg cgagctcctg gccggggttc gatagcgctc cggtcgggac gctacccgag
     2281 cagcagcgag accagctgga gcaggagagc cagccccagg agccacgagg cgctcgtccg
     2341 gcgcacctgg tgacggatca gccggacgcc cgccacgagt ccgatgagga gcgcggcacc
     2401 actcagcacg ctgcgcgggg ggaacgagac cggctcgcgc ccgagcaggc tcgccagtag
     2461 ccggccctcc gttctcgccg gctgctcgcg ggcggtgacg tccagcgtgc aggtcgcccg
     2521 gctcggctga gaaccctccg aggtgcccgt gagcacgagc tgaccggcgc tcaagggcgg
     2581 cacgtcgatc tcgccgaact ggatggacag gggaggccgg tgtcccgacc ggccgggatc
     2641 gacgaagcgg tcgatggtgg acaccgagcc gtgcagaagg tcgcggcccc tcttgacgtc
     2701 gcgccaccgc tcgacgaatc cggcatagcg agccgacacg cctccgttat ccaggatgca
     2761 ggccccggcg acctgcacgt gctcgagcag cgtgaatcgc tgcgccggcg aggcgggttg
     2821 gcggggctcg gcggcacacc cggcgagccc tccgacgagc agcgccatcg tcaccgtccg
     2881 gaagccccgc gagaaagcca ttgccctaag ctcacgccac aaccgggccg cgtcaagtcg
     2941 ccccgagatc ggaccgatca tttaaccctc ggaggcatgc catgcttgag ggaccatggg
     3001 gcgcaagggg tggggacgtc tgggggctgg cctggccgcg gcgctcgggc tcgcggggtg
     3061 cgcgacgggg tccggggcct acaccgagcc cgagcaggcc cggatcgacg cgtacgagcg
     3121 ggcggccgag caaattcttc agtcccgtgg catgaggggg cccgccccgg ccgtgcgcgt
     3181 cgggaccgac ccctcgctcg ccggcgcggg acgcccggcc gggtactaca ccgaccaggg
     3241 gcgccgcatc gtggtcaacc gcgcggcggt ggccgacgac tacatcgccc aggcggtcct
     3301 gagccaggag ctggcccact acgtcctggg ccacgccgac aacgcctgcc gggagcggcg
     3361 cctcgaatgc gagatcgagg cccgcatcgc gagcgtcgag ctgctcatga ccggctgggg
     3421 cctcgagtac tcggatgcga tccgcctgca gtacgcctat ctgaagagcg tggtcctggc
     3481 cgcccagcgg ggggaggtcc cggccacgcc cggcaagagc gacccctgcc gggagctcca
     3541 ggagttcgcg acacggttca aggctcaggc ttcgtgcgaa taggccggcc gtgtatcgga
     3601 atcttcccac tgggacgcac agttggatcg ccgggggcat ccaattccct cggcttgacc
     3661 cccctctcgc tgctagacta gaatccgcaa atcgcccaat ggccgcacaa ggggaactgt
     3721 ggtctcatga ggggcttcgg tgaagattaa ggtcctcggg tcgttcggga gcgaaggcct
     3781 ggggcagagg ccctcggcgt tcctcgtcga cgaccgggtc ctggtggacg ccggcaccgt
     3841 gggcggcgcc ctctcggtgg ccgagcagat cgagatccag ttcgccctga tcagccacgc
     3901 ccacctcgac cacaccgcgg gcctggcctt cctcaccgat accctggcga tggtggcggc
     3961 ggaccgccgg gtgaccgcct gcagcatcgc gccggtcatc gaggcgctcc ggacccacgc
     4021 cttcaacgac gcgctctggc cgaacttcac gaacatcccc tccgcggccc agtcggtgct
     4081 gggcttccgg accctcccgg aggaggctga gtcgcgggtg ggcgagctgt gggtgacccc
     4141 gatactggtc gagcacacgg ttcccaccac cggcttcgtg atccacgacg gcgagaccgg
     4201 gttcgtgttc agcggggaca ccggtcccac caagcggatc tggcagatcg cccgcgagat
     4261 gcgcgggctc aaggccctga tcgtcgagac cgccttcccc aaccggctcg ccgccctcgc
     4321 caaagcctcc ggccacctga cgcccgagat gctgggccag gagatcgcga agatgccggc
     4381 cgacctgccg atctgggtct accacatcaa gccgcagctc tatcaggaga ccgcggagga
     4441 gctggccaag atcgacccca gccgcatcca catcctcgag caggggaaga cctacacgct
     4501 ctagtccccg ccgtcgatca gccggctcag ccgctcgcgg tacggaaaca ggctgaagag
     4561 ccccccggtg tgaatgaagc agacgcgctg gcccagcgcc ttcgggtcgc gcgagagcgt
     4621 ctccaccagc ccccggaacc cgcgcgcggt gtacaccgga tcgagcagca cgccctcctc
     4681 ccgggccagc tccacgagca cggccaggtc cgcatcggtc gcgcccaggc cgtcgccgcc
     4741 cggatcgccg tccagcagat ggaccgtctt cggcacgtcg atggagaacc cccaccgccg
     4801 gatcgcggcg cccatcgtgt gcacgacgtg ctcccgcacc cgctcggccg gacgcgcgat
     4861 cggcacgccc acgatctccc cgggcaaccc ggccagccgc ttgccgagca agaggcccgc
     4921 ctggctgccg ccggtgaacg cgctgatgac caccgcgtcg aagcgcggcg ccccgtggtt
     4981 gatctgctcg ctcagctcga ccgcgcactc gaggtagccg agcgcgccca cctcgttggc
     5041 gccgctctcc gggatcaggt acgggcgccc cccgcgcgcc cggatctcgg tcgccatcgt
     5101 ctccatgatt tcccccgccc gcgcgaagtc ggcctcggcg cagtagcgca cctcggcccc
     5161 gagcagacgg ccgagcagga ggttgccgtc gtaggcgacg ggcggctcgc cccgcaccag
     5221 gagcaccgcg cgcagcccga gccgcgcggc caccgcggca accgcgcggc agccgttgga
     5281 ctggagcgcg ccgcacgcga ccagggtgtc ggcgccctgc tgcagcgcct cccccaccag
     5341 gaactcgagc ttgcggacct tgttgccgga ttcgaggagg ccggtcaggt cgtcgcgctt
     5401 gacgtggagc tccaccccca ggcggcggga gagccgatcg agcttgagaa tcggcgtcgg
     5461 gccgagggcg agctcgagcc gggcgggcgg tgcggccatg aaccggctac gcgtgctccg
     5521 tcttcagatt ggccgcgatc ggcgcgaaat cgtacgcggg gaacacctgg tagcggaagg
     5581 cgccccaggc cttctgctcc atgtagcgat tcagcgcgcg taccttgtcc ggctcgacgc
     5641 ggagctggat gatgaagccg aacgacatcg ccaccaccca cgagaccagc tcgctcccct
     5701 cgggcggaaa gtcgcggtag aatccggtct tcttgaggtg cgccatgatc tcgtcgaggg
     5761 gcttggactg gtcgtggtgg agcagcacgg tcaggagcac gcgatcgttg gtcgccatgg
     5821 cccgcagggt accctatttc cgcggctact cgatgacctg atccgcccgc gccagccgcg
     5881 atcgtgggga tcttgtccac gtagatggcg gcgcgccgga agaggtcgcg aagatccgtt
     5941 ccgtaggaca tgaggccgcc gtgcgctacg aagtcgctcg catcgaacat ctcgggcagg
     6001 cgatgccggc tgccgacctc gacgatgcgc ttcggcggcg agaagaagaa cccgacgcat
     6061 cgaacgtcac cggggacacg atcggatcgt ccgtgtcccg cgcggcgccg cctcgaccgc
     6121 ctgcgtgctc gcggccacga tgacgtcgac gcttccgtcc gcatttcgga gcccgacgtg
     6181 aacgttttct gccccacgac gtagcccggc tcccccaggt cggtggggaa cagcaggata
     6241 cccggcggct cacccgcgct gcgttaggcg cggcgctcac cctgtaatct ccgggggttg
     6301 cggcccaggg cccaacttca ggcattgaca ccagcccgcc ctccggttac cgtgcctgtc
     6361 atgcgtatcg aggtgcacga caatcaggtc gagtccgcgc tcaagaccat gaagaagctc
     6421 atgctcaagg atgggctctt caaggagatg aagaagcgcg cgtactacga gaagccgtcg
     6481 gtcaagcgca agcggaagca ggccgaggcg cgcaagaagc gccgcaaggc catgaagcgg
     6541 gccgaggcgg acgactgagc tctcccgggc ctcccggccc ggttccccct actctcgggt
     6601 gactccaccg cgggagtccc ttgccagcca gcggctctgg ctcgccttcg cgaccatgct
     6661 gctggtggcc ggaatcacca ataccttccc ggtgttcctg cccgccctcc tggccgagtt
     6721 cgggggctcg cgcgccgcca ccgcctcgac cgcctcgctg ctctggatcg gcggagccct
     6781 gttgagcccg gtggccggct acctggtcgc gcgctggaac cctcgacggc tggtctgcct
     6841 cggcctggcc atcgtggccg ggggcatgct cctcgggagc ctcgccccga ccctgcccct
     6901 gttcctggcc gcgatgggca tcgggggtgg catcggcctg ggtctgaccg gcatgaccac
     6961 ccacgccgcc ctgatcgccg acgcctacgt gcgccgccgg ggcctggcca ccggcatcgc
     7021 cttcggcggg tcgatggccg cgtacgccct ggcgcccccg gcccagtgga tcatcaccca
     7081 ctggggctgg cggccggcct tctggtgcta cgcagcggcg atcctggcgt tgatcccgtg
     7141 ggcgtggcgc gcgcacccca cgcacctcgc ctcgatccgc aatggcggca ccggctcgtc
     7201 ggagagcgcc gcggtgacca tcgccagcat cgtgcgctcg gccggcttct ggagcctctt
     7261 cgtgatgttc accacgcctc cgctcttcgg ctacctggcc accacgcagc acgcgctcta
     7321 cttcgtcgag cgcggcttct ccgcgggcca ggcctcggtg ctcctggcca tcggcggcct
     7381 cctggccggc gccggccgcg cgctcgccgg cctgaccgcc gaccgcctcg gcggccccac
     7441 cgcgggcttt ctctcgttct cctgctcgct cgtggggatg gggtgcctga tcgggatgga
     7501 gttccgtccg gtgtggttgc tggcggcggg gtacgtcttc ttcctgttcc tgccgctcgg
     7561 ctcgcgggcg agcatcgtca ccgtgctgct cgggcgcatc gccccgcccg cgcagtacgg
     7621 cgtgatcttc ggcctgctcg gcatcggcaa caacctgggg gcggcgctcg gcccgtggct
     7681 ctccggcatc ctcttcgacc ggaccggttc ctacctggtg
//
LOCUS       DATMCV010001099         3920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_727190, whole genome shotgun sequence.
ACCESSION   DATMCV010001099 DATMCV010000000
VERSION     DATMCV010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727190"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VNU03_25150"
     CDS             <1..679
                     /locus_tag="VNU03_25150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP77833.1"
                     /translation="PMQSVDLAVDEMRYAREQLGMRGGFLRPNPYHGKKMISDPMYEP
                     FWMIAEELDFSIGFHEGSTNAMPTVGVDRFEDDRAARHMISHTMEMMLVALSVIWGGV
                     VDRHPRLRVAFLESGGGWIAPWLDRMDRHFDDQGFNESAPKTRPSELFQRNCWISFEP
                     VEGCLGVLADYIGPHKILWATDYPHPDGFFPGAPAMIAGRPELSDATKREILGGGARR
                     FYGLEAP"
     gene            676..1884
                     /locus_tag="VNU03_25155"
     CDS             676..1884
                     /locus_tag="VNU03_25155"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP77834.1"
                     /translation="MTDHALSGIRILDLTQFEAGTSCTQLLGWLGADVIKIEPPGGEQ
                     SRRNRPEVPGLDAMFFLLFNANKRSVTIDLKNPEGHALFLRLVEGADVVTENFAPGLM
                     ERLGLDYEQLAAVNPRIILARLKGFGLSGPYHEYKSFDMIAQAMGGVMSVTGFPDREP
                     VLCGANIGDSGAGVHMAAGIMAAIIERQRTGRGQVVEVSMQEAVANLIRQRYVVHYRD
                     GKPTPRRGNGAPPGAVPDGLYACAPGGPNDYVYIYVQPMNRGMWEDFARAIGREDLIA
                     DPRCVDATSRWAHRDALNDIARAWTGARSKQTVLETLGKAGVPCGAIFDTQEALDDPH
                     MNARGAIETIEHPTRGRFRVPGCPIRLSSGKAATTPPPLAGQHTDEVLAEVLEMSPAA
                     VAELRARGAI"
     gene            1888..2586
                     /locus_tag="VNU03_25160"
     CDS             1888..2586
                     /locus_tag="VNU03_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HWP77835.1"
                     /translation="MHFGHSPEIWRQFPQLVPGVLVVGGIDAHADVQALLTPWYERAR
                     ERLARGPESELAEVSAWRRAYAQMGLKPTQYRSAAEALLRRLRREGDLPRVHPLVDLG
                     NALSLAFALPVAIFDLAGVAGALEVRPAEGGEEHVGFSGEIERAEPGEVIFADAAKHA
                     HARRWTFRQSRRSTVSASTRDVLIVAEGLHPTAGADVPALIDALVDGLARIGTAPRHR
                     AVLTAAAPRLELAV"
     gene            2625..3275
                     /locus_tag="VNU03_25165"
     CDS             2625..3275
                     /locus_tag="VNU03_25165"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HWP77836.1"
                     /translation="MARSLPAAAAVAVFGTLYGAAARGILGGPLTLLSSVIVYSGALQ
                     FAMVAMLGVGTAALPLLLTALVLNLRHVVLGAALRARFESGPLGRAGLAFFLVDETFG
                     FAIAAGNAVEPAAVGRTTERTLLVAGLTLYVAWLAGTLVGVLAGGLTAVESAAGAIFP
                     VLFIGLASLAATRRSHVVRALAAAVLTALVAWLLPGLRMLAPVVAGVLVALPGRER"
     gene            3272..3565
                     /locus_tag="VNU03_25170"
     CDS             3272..3565
                     /locus_tag="VNU03_25170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HWP77837.1"
                     /translation="MSPLLLVAIALITYGSRAAAVVLLPRPGPRFETVLSRIPAAIFG
                     GLATATLLGEGATLAGSPILGAAAGALAATPARSLLLCLVGGAIGYGVGALLG"
     gene            complement(3581..>3920)
                     /locus_tag="VNU03_25175"
     CDS             complement(3581..>3920)
                     /locus_tag="VNU03_25175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWP77838.1"
                     /translation="LLEHMRIAQRDILEYIEDPEYRLPAWPDDYWPKADRQADAAAWK
                     KTVADFRRDRAALEKIAADPRVALGSALPYASQHTVIRELLIASEHNTYHIGELAILR
                     QVMGTWPARR"
BASE COUNT          488 a         1449 c         1404 g          579 t
ORIGIN      
        1 gccgatgcag tcggtcgatc tagcggtcga cgagatgcgc tatgcccgcg aacagctcgg
       61 gatgcgcggc gggtttctgc ggcccaaccc ttatcacggc aagaagatga tcagcgaccc
      121 gatgtacgag ccgttctgga tgatcgccga ggaactcgac ttctccatcg gctttcacga
      181 aggatcgacc aacgcgatgc cgaccgtcgg cgtcgaccgg ttcgaggatg accgcgcggc
      241 gcgccacatg atctcgcaca cgatggagat gatgctcgtt gcgctgagcg tgatctgggg
      301 cggtgtcgtc gaccgtcacc cgcgattgcg cgtggcgttt ctcgagtcgg gcggcggctg
      361 gatcgcgccg tggctcgacc gcatggaccg gcacttcgat gaccagggct tcaacgagtc
      421 cgcgccgaag acgaggccga gcgagctgtt ccagcgcaac tgctggatct ccttcgagcc
      481 ggtcgagggc tgcctcggcg tgctcgccga ctacatcggc ccgcacaaga tcctatgggc
      541 gaccgactac ccgcatccgg acggcttctt ccccggcgcg cccgcgatga tcgcggggcg
      601 gccggagctg tcggacgcca ccaagcgcga gatcctgggc ggaggcgccc ggcgcttcta
      661 cggcctggag gccccgtgac cgaccacgct ctatccggca tccggatcct ggatctcacc
      721 cagttcgagg cgggcacctc gtgcacccag ctcctgggct ggctcggcgc cgacgtcatc
      781 aagatcgagc ctccgggcgg cgagcagtcg cggcgcaacc ggccggaggt gcccggcctc
      841 gacgcgatgt tcttcctgct cttcaacgcc aacaagcgca gcgtcaccat cgacctcaag
      901 aacccggagg gccacgcgct cttcctgcgc ctggtcgagg gcgcggacgt ggtgaccgag
      961 aacttcgccc ccgggctgat ggagcggctc gggctcgact acgagcagct cgccgcggtg
     1021 aacccgcgca tcatcctggc ccgcctcaag ggcttcgggc tctccgggcc gtatcacgag
     1081 tacaagagct tcgacatgat cgcccaggcg atgggcgggg tgatgagcgt gaccggcttc
     1141 cccgatcgcg agccggtgct ctgcggcgcc aacatcggcg acagcggcgc cggggtgcac
     1201 atggcggccg gcatcatggc cgcgatcatc gagcggcagc ggaccggccg cggccaggtg
     1261 gtcgaggtct cgatgcagga ggcggtggcc aacctcatcc gccagcgcta cgtcgtccac
     1321 taccgcgacg gcaagcccac cccgcgccgg ggcaacgggg cgcctcccgg cgcggtgccc
     1381 gacggcctct acgcgtgcgc gccgggcggg cccaacgact acgtgtacat ctacgtgcag
     1441 ccgatgaacc ggggcatgtg ggaggacttc gcgcgggcca tcggccgcga ggatctgatc
     1501 gccgacccgc gctgcgtgga tgcgacttcc agatgggctc atcgagacgc gctcaatgac
     1561 atcgcccgcg cctggaccgg cgcgcgctcg aagcagacgg tgctcgagac gctcggaaag
     1621 gccggcgtgc cctgcggcgc gatcttcgac acccaggaag cgctggacga cccgcacatg
     1681 aacgcgcgcg gcgcgatcga gacgatcgag catcccacgc gcggccgctt ccgggtgccg
     1741 ggctgtccga tccgcctctc gtccgggaag gcggcgacca cgccgccccc gctggccggg
     1801 cagcacaccg acgaggtgct ggccgaggtg ctggagatgt cgcccgcggc ggtggcggag
     1861 ctgcgcgcgc gcggcgcgat ctgatcggtg cacttcggcc actcgccgga gatctggcga
     1921 cagttccccc agctcgtgcc cggcgtcctc gtggtcggcg gcatcgacgc ccacgcggac
     1981 gtgcaggcgc tcctcacccc ctggtacgag cgggcccgcg agcggctcgc gcgcgggccg
     2041 gagtcggagc tggccgaggt gtcggcgtgg cgacgcgcct acgcccagat gggcctcaag
     2101 cccacccagt accgctcggc ggccgaggcg ctgctgcgtc gcctccggcg tgagggcgac
     2161 ctgccccgcg tgcacccgct ggtggatctc ggcaacgcgc tctcgctcgc ctttgcgctg
     2221 ccggtggcga tattcgatct ggccggggtg gccggcgccc tggaagtccg gccggcggag
     2281 ggcggcgagg agcacgtggg cttcagcggc gagatcgagc gtgcggagcc cggcgaggtg
     2341 atcttcgccg acgcggcgaa gcacgcccac gcgcggcgct ggacgttccg gcagagccgg
     2401 cgctcgacgg tctccgcctc cacccgcgac gtgctgatcg tcgccgaggg actccacccc
     2461 accgccggcg ccgacgttcc cgccctcatc gacgcgctcg tcgacggtct ggcccgcatc
     2521 gggaccgccc cgcggcatcg cgcggtgctc accgccgcgg cgccacgact cgagctggcc
     2581 gtctgagccc ggctccgacc ggggtccccg cgatccgcac catcgtggcc cgcagcctgc
     2641 ccgccgcggc ggcggtcgcg gtgttcggca ccctctacgg cgcagccgcg cgcggcatcc
     2701 tcggcgggcc gctgaccctc ctctcctcgg tcatcgtcta ctccggcgcg ctccagttcg
     2761 cgatggtggc catgctcggc gtgggcaccg ccgcgctgcc gctgctgctg accgccctgg
     2821 tgctcaacct ccgccacgtg gtgctgggcg cggccctgcg ggcgcgcttc gagagcgggc
     2881 cgctcggccg ggccgggctc gccttcttcc tggtcgacga gacgttcggc ttcgcgatcg
     2941 cggccggcaa cgcggtggag ccggccgcgg tgggccggac caccgagcgc accctgctgg
     3001 tcgccggcct cacgctctac gtggcgtggc tcgcgggcac gctggtcggc gtgctggcgg
     3061 gcggtctcac cgcggtggag agcgcggcgg gcgcgatctt cccggtgctg ttcatcggcc
     3121 tcgcctccct tgccgccacg cgccgctccc acgtggtgcg cgcgctcgcc gccgccgtcc
     3181 tcaccgccct ggtggcctgg ctgctgcccg gcctgcgcat gctggccccg gtggtcgcgg
     3241 gagtcctggt cgccctgccg gggcgcgagc gatgagcccg ctgctcctcg tcgcgatcgc
     3301 gctgatcacc tacggcagcc gcgccgccgc ggtggtgctg ctcccgcgcc cgggcccgcg
     3361 cttcgagacc gtgctctccc gcatccccgc cgcgatcttc ggcgggctcg ccaccgcgac
     3421 gctcctgggc gagggcgcca ccctggccgg ctcgcccatc ctgggcgccg ccgccggcgc
     3481 gctggcggcc accccggcgc gctcgctgct gctctgcctg gtcggcggcg cgatcggcta
     3541 cggggtgggc gcgctgctcg gctgagcgcg cccggagcgc tcagcggcgg gcgggccagg
     3601 tgcccatgac ctggcggaga atcgcgagct cgccgatgtg ataggtgttg tgctcggagg
     3661 cgatcagcag ctcgcggatc acggtgtgct gcgacgcgta cggcagcgcg gagccgaggg
     3721 ccacccgggg atcggccgcg atcttctcga gcgcggcgcg gtcccgccgg aaatcggcca
     3781 ccgtcttctt ccacgcggcc gcgtccgcct gccggtccgc cttcggccag tagtcgtcgg
     3841 gccacgcggg caggcggtac tccgggtcct cgatgtactc caggatgtcg cgctgggcga
     3901 tgcgcatgtg ctcgagcaga
//
LOCUS       DATMCV010001100         4074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_327924, whole genome shotgun sequence.
ACCESSION   DATMCV010001100 DATMCV010000000
VERSION     DATMCV010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327924"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VNU03_25180"
     CDS             <1..301
                     /locus_tag="VNU03_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HWP77839.1"
                     /translation="EEAYYRVDRMLTQLWLAGALDGVAGMVFGKFTDCDPSPFFLQNR
                     PLEEILAERLRALGIPTVSGVMIGHIDDQATLPVGCLAELDADAGTLTLLEPGVS"
     gene            complement(311..1750)
                     /locus_tag="VNU03_25185"
     CDS             complement(311..1750)
                     /locus_tag="VNU03_25185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWP77840.1"
                     /translation="MGRVLALVTTLVLLCSPLSASAQDPRAALERASRALGAGTLRTL
                     QYSANGVSFAVGQSVVPGSAWPRFNIPSFTRTISYETASLREEQVRSRAEMPPRGGGV
                     PAVGEARQIALVSGDLAWNVVGESVAPSPVALVERQLQLWTTPHGVIWAAMASNATVQ
                     GRTIAFAVPGRYTVRATLDGAGLIEKVEAVLSSPVVGDMPVEIRYSDYREFGDVKFPT
                     RIRQSAGGFPTVDFTVTDVRPNTPVDIQVPDAIRQATKPYARVSSEAAADGVWYVTGG
                     SHHSVVIEMKDHVIVVEAPLNDERATAVIAEARRLVPGKPIRVVINSHHHFDHAGGLR
                     AFAAEGIEVITHETNRAFFETALVAPATMTPDRQQAAQRKVAVEGVRDKRVLTDGTRA
                     VEIHHMAGNTHADGMLLVYLPKERLLIQADAFTPGPPNAPTPAIINPLSVDLADQITR
                     LGLSVDRLLPLHGRMVPLADLQRAIGRGN"
     gene            complement(1957..2352)
                     /locus_tag="VNU03_25190"
     CDS             complement(1957..2352)
                     /locus_tag="VNU03_25190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77841.1"
                     /translation="MMTPSVVTLQEVLRHHRTLSRRGREFYESLLHLGDVLAVRVDYL
                     PGAMLWLVTSPTQAQLMRKAREGRSTSDVCVMSLREAQDLFGAVGDPTPATLYEVAGW
                     LLAPAPDELAAIVEDDEQSEDQPEDQDAL"
     gene            complement(2502..3434)
                     /locus_tag="VNU03_25195"
     CDS             complement(2502..3434)
                     /locus_tag="VNU03_25195"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWP77842.1"
                     /translation="MIDEPLVRFTAHQLPRQELHPLMARSDGPAFRRAAAHLGALAVT
                     GALLWRLRATGWALPLLVAHGYVLAFVFCAFHETAHRTAFRTRWLNTALGTLAGLLIV
                     WPYRNYRVYHWEHHRFTQDEARDPELYFAKPTSVGAYLLALTGVPNLIRRVSDLLRLA
                     GGRADRPWMVAPERRPLIREARIYLGVYLAVALASALTGSPIVLLLWIAPLLVGQAFL
                     RPYLLAEHTACGSTRDGLENTRTTRTLALVRLFAWNMPYHAEHHAYPAVPFHALPRLH
                     GHVRARLANLEPGYVAATVKVNRYLFARARGPAE"
     gene            3488..3757
                     /locus_tag="VNU03_25200"
     CDS             3488..3757
                     /locus_tag="VNU03_25200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77843.1"
                     /translation="MPKRLPPRKVPGRRPPHWLVIVARDEDEIHAKLQRVLAADRHVR
                     VIFDRRGDTSRNPPWVTRSLGLHGFAVVLAARKGRSKPDRAVASG"
     gene            complement(3772..>4074)
                     /locus_tag="VNU03_25205"
     CDS             complement(3772..>4074)
                     /locus_tag="VNU03_25205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77844.1"
                     /translation="QRGAFGYDSRRMLSRAQEARVRALIWTSSPGTLLTYCLEHLATS
                     AKIPASHMSDLAVFVRSLHERGDCQRRFGGFCEAGRHETRQTLVWGPSPRLRAEGT"
BASE COUNT          612 a         1419 c         1464 g          579 t
ORIGIN      
        1 cgaggaagcc tactaccggg tcgaccgcat gctgacccag ctctggctcg ccggcgcgct
       61 ggacggcgtg gccggaatgg tcttcggcaa gttcacggac tgcgacccgt cgccgttctt
      121 cctgcagaac cgtccgctcg aggagatcct cgccgagcgg ctccgggccc tcggcatccc
      181 caccgtcagc ggggtgatga tcggccacat cgacgatcag gccaccctgc ccgttggctg
      241 cctcgccgag ctggacgccg acgccgggac cctcaccctc ctcgagccgg gggtgagcta
      301 gcgctcctcg tcagttgccg cggccgatcg cccgctgcag gtcggccagc ggcaccatcc
      361 gcccgtgcag tggaagcagc cgatccaccg agagcccgag ccgcgtgatc tgatcggcca
      421 gatccacgct cagcggattg atgatcgcgg gcgtcggagc gttcggcgga ccgggggtga
      481 aggcgtcggc ctggatcagc agccgctcct tcggcaggta cacgagcagc atgccgtcgg
      541 cgtgggtatt gccggccatg tgatggatct cgacggcgcg cgtgccatcg gtgagcaccc
      601 gcttgtcgcg cacgccctcg accgcgacct ttcgctgggc ggcctgctgc cggtccggag
      661 tcatggtggc gggcgccacc agggccgtct cgaagaaggc ccgattcgtc tcgtgggtga
      721 tcacctcgat accctccgcc gcgaacgcgc gaagaccgcc cgcgtggtcg aagtggtggt
      781 ggctgttgat caccacgcgg atcggcttgc ccggcaccag acggcgcgct tccgcgatca
      841 ccgcggtcgc ccgctcgtcg ttgagcgggg cctccaccac gatgacgtgg tccttcatct
      901 cgatcaccac gctgtggtgg ctgccccccg tgacgtacca caccccgtcc gccgcggcct
      961 cgctgctcac gcgcgcgtag ggcttggtcg cctgtcggat ggcgtccggt acctggatgt
     1021 ccaccggcgt gttcgggcgg acgtcggtga ccgtgaagtc caccgtcggg aacccgcccg
     1081 cggactgacg gatgcgggtc gggaacttca cgtcgccgaa ctcgcggtag tcggagtatc
     1141 ggatctcgac cggcatgtcg cccaccaccg ggctcgacag caccgcctcg accttctcga
     1201 tcagcccggc gccgtcgagg gtggcccgta ccgtgtaacg gccgggcacc gcgaaggcga
     1261 tggtgcgccc ctgcacggtg gcattggagg ccatcgcggc ccagatcacc ccgtggggcg
     1321 tggtccagag ctggagctgg cgttccacca aggccaccgg cgacggcgcg acggactcgc
     1381 ccaccacgtt ccacgccaga tccccgctca ccagtgcgat ctgccgcgcc tcgccgaccg
     1441 cgggaacgcc cccgccgcgc ggcggcatct cggcccgcga ccgcacctgc tcctcgcgca
     1501 gcgacgcggt ctcgtagctg attgtccgcg tgaaggacgg gatgttgaac cgcggccagg
     1561 ccgagcccgg caccacgctc tggcccaccg cgaagctcac cccgttcgcg gagtactgga
     1621 gcgtcctgag ggtgccggcc cccagcgccc gggaggcgcg ctcgagagcg gcccgcggat
     1681 cctgggcgga agcggagagt ggagagcaca gcagcaccaa ggtggtgacc agcgcgagca
     1741 ctctgcccat ctcgggcctc cttgtcgtcg gggtcaaggt cgatgcgcgg tccagaaccg
     1801 cgtcagctcg tcggccaccg tgtcgggccg ctcgagctgc caccagtggg agcagccggg
     1861 gatgcagacg aagacggcgg gcatgctggt cggcatccta gggatgccgc gacggcgagt
     1921 caagcgagga cggcggggcg ggtggagcga gtcgggctag agcgcgtcct ggtcttcggg
     1981 ctgatcttcg gactgctcgt cgtcctcgac gatggcggcg agctcgtcgg gggccggcgc
     2041 gaggagccag ccggcgacct cgtagagcgt ggccggggtc ggatcgccca ccgcgccgaa
     2101 caggtcctgg gcttcccgca ggctcatcac gcacacgtcg gacgtggatc gcccctcgcg
     2161 ggccttccgc atcagctggg cctgcgtggg actggtcacg agccagagca tcgcgcccgg
     2221 caggtagtcc acccgcacgg ccagcacgtc gcccagatgg agcaggctct cgtagaactc
     2281 ccggccgcgg cgcgacagcg tccggtggtg cctcagcacc tcctgcagcg tgaccacact
     2341 cggcgtcatc attggcgtgt accttgacca cgcaaaaaag caaacgccgc gccacttcag
     2401 gaaatgcacg aagtcagcta gttgcgaagc actctcgacc atacgcccgc gccggattgt
     2461 cagccggggt gccgcagaat gctagcgagg cgcccgaccg gctactcggc ggggccgcgc
     2521 gctcgggcga acaggtagcg gttcaccttg acggtggccg cgacgtagcc gggctcgagg
     2581 ttggccagcc gggcccggac gtgcccgtgc aagcgtggca gcgcgtggaa gggcaccgcc
     2641 gggtaggcgt ggtgctcggc gtgatacggc atgttccacg cgaagaggcg cacgagggcc
     2701 agggtgcggg tggtgcgcgt gttctccagg ccgtcgcggg tggatccgca cgcggtgtgc
     2761 tcggccagga gatacgggcg gaggaacgcc tgcccgacca gcagcggcgc gatccagagg
     2821 agcagcacga tcggcgagcc ggtcagcgcg gaggcgaggg cgaccgcgag gtagacaccc
     2881 aggtagatgc gcgcctctcg gatcagcggc cgacgctccg gcgcgaccat ccacggccgg
     2941 tcggcccgac cgcccgcgag ccggagaaga tcgctcacac gccggatcaa gttcggcacc
     3001 ccggtcagcg ccagcagata ggcgccgacc gacgtgggct tcgcgaagta gagctcgggg
     3061 tcacgcgcct cgtcctgcgt gaagcggtga tgctcccagt ggtagacgcg gtagttccgg
     3121 tagggccaga cgatcaggag gccggccagg gtcccgagcg cggtgttcag ccagcgggtg
     3181 cggaaggcgg tgcggtgcgc ggtctcgtgg aacgcgcaga agacgaaggc gagcacgtag
     3241 ccgtgggcga ccagcagggg cagcgcccac ccggtggcgc gcaggcgcca caggagcgcc
     3301 ccggtgaccg cgagcgcgcc caggtgcgcc gcggcgcgcc ggaaggcggg tccgtcggag
     3361 cgcgccatga gggggtgcag ctcctggcgc ggcagctgat gcgcggtgaa gcgaacgagc
     3421 ggttcgtcga tcatgagatt tcgctactat ccgggtctgg cccgactccg atacgatccg
     3481 tcccgcgatg ccgaagcggc tccctccgcg caaggtccca ggccgccgac cgccccactg
     3541 gctcgtcatc gtcgcccgcg acgaggacga gattcacgcc aagctccagc gcgtcctggc
     3601 ggccgaccgt cacgtccggg tgatcttcga tcgccggggc gacacgtccc gcaatccgcc
     3661 ctgggtcacg cgcagcctgg gcctccacgg cttcgcggtc gtcctggccg ctcgcaaggg
     3721 ccgatccaag ccggatcgtg cggtcgcctc gggctgattg ggcggagccc gtcaggtccc
     3781 ctcggcccgc agccgaggcg acgggcccca gacgagggtc tggcgcgttt cgtgccgccc
     3841 ggcctcacag aagcctccga agcgccgctg gcagtctccc cgctcgtgca ggctgcggac
     3901 gaagacggct aggtccgaca tgtggctggc cgggatcttg gcgctggtcg ccaggtgctc
     3961 gaggcaatag gtcaagagcg tgccgggcga gctcgtccag atgagcgcac ggacgcgagc
     4021 ttcctgggcg cgggagagca tgcgccggct atcgtagccg aacgcgcccc gctg
//
LOCUS       DATMCV010001101         2022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_328151, whole genome shotgun sequence.
ACCESSION   DATMCV010001101 DATMCV010000000
VERSION     DATMCV010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328151"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VNU03_25210"
     CDS             complement(<1..192)
                     /locus_tag="VNU03_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWP77845.1"
                     /translation="MVGAPAFLAEVVIGGLAAGLMYSLVALGFVLIYKASGVFNFAQG
                     VMVLFAALTLVGLMERGAPL"
     gene            complement(230..1036)
                     /locus_tag="VNU03_25215"
     CDS             complement(230..1036)
                     /locus_tag="VNU03_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018301948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77846.1"
                     /translation="MREPTSPPPVASATPLLEVDEVSVRFGAIQALDRVSLEIGRGEI
                     VAIIGPNGAGKTTLLNVISGFYHPTEGQIRFEGRDRTRLRPYDVAALGVARTFQNVAL
                     FKGMSVLDNIMTGRLLKMHGSFLWEALYWGPAMRQELAHREFVERIIDFLEIEAIRKA
                     GAGTLPYGLQKRVELARALAAEPKLLLLDEPMAGMNVEEKEDMCRFVLGVNDEFGTTI
                     ALIEHDMSVVMDISDRVVALDYGRKIADGRPDEVRADQGVIDAYLGVSHA"
     gene            complement(1033..>2022)
                     /locus_tag="VNU03_25220"
     CDS             complement(1033..>2022)
                     /locus_tag="VNU03_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP77847.1"
                     /translation="AHRAPALLDRLLYPLGWLLVYGPLRDNLGMGKIRVAYTAGEAIG
                     PELFEFYRSLGVNVKQLYGMTESSALICIQQDGDVKLDTVGPPLPGVEVRVSPSGEVL
                     YRSPGVFQGYYKNPDATRETLVEGWVHSGDAGFFDKDGHLKIIDRARDVGRLAGGTIF
                     APKYLENKLKFSPYIKEAVCVGDGRPFVAALLNIDLVSVGNWAERRGLAYTSYTDLAQ
                     KPEVYELIRQEVVRVNRSLLEDEALRGAQIRRFLLLHKELDPDDQEITRTRKVRRGFI
                     GQKYAPLIEALYGTGDSVQVEAQVTYEDGRTATVRAAVRLGEAETFAVTGASA"
BASE COUNT          306 a          727 c          680 g          309 t
ORIGIN      
        1 cagcggggcc ccgcgctcca tcagcccgac cagggtcagg gccgcgaaga gcaccatgac
       61 cccctgggcg aagttgaaga ccccggaggc cttgtagatg agcacgaagc ccagcgccac
      121 cagggagtac atgaggcccg cggcgaggcc gccgatcacc acctcggcca ggaacgcggg
      181 ggcgcccacc atgtccttca cgggcccgac cagcaccccg tagaggacgt caagcatggc
      241 tcacccccag gtaggcgtcg atcacaccct ggtcggcgcg cacctcgtcg ggccgcccgt
      301 ccgcgatctt ccgcccgtag tcgagggcca cgacccggtc cgagatgtcc atgaccacgc
      361 tcatgtcgtg ctcgatcagc gcgatcgtgg tcccgaactc gtcgttgacg ccgaggacga
      421 agcggcacat gtcctccttc tcctcgacgt tcatgcccgc catcggctcg tccagcagca
      481 ggagcttggg ctcggcggcc agggcgcggg ccagctccac ccgcttctgc agcccgtagg
      541 gcagcgtgcc cgcccccgcc ttgcggatgg cctcgatctc caggaagtcg atgatgcgct
      601 cgacgaactc ccgatgcgcg agctcctgcc gcatcgcggg gccccagtag agcgcctccc
      661 acaggaagct gccgtgcatc ttgagcagcc gcccggtcat gatgttgtcg agcacgctca
      721 tgcccttgaa gagagccacg ttctggaagg tgcgggccac gccgagcgcc gccacgtcgt
      781 agggccgcag gcgcgtgcgg tcgcgtccct cgaagcggat ctgaccctcg gtcgggtggt
      841 agaagccgct gatcacgttg agcagcgtgg tcttgcccgc cccgttgggg ccgatgatcg
      901 cgacgatctc cccgcgcccg atctcgaggc tcacccggtc gagggcctgg atggcgccga
      961 accgcacgct gacctcgtcc acctccagca gcggggtggc ggacgcgacc ggcggcgggc
     1021 tcgtgggctc cctcacgcgg aggcccccgt gaccgcgaac gtctccgcct cgccgagccg
     1081 caccgcggcc cgcacggtgg cggtgcggcc gtcctcgtag gtcacctgcg cctcgacctg
     1141 gaccgagtcg ccggtaccgt agagcgcctc gatgagggga gcgtacttct gcccgatgaa
     1201 gccgcgccgc accttgcggg tccgggtgat ctcctggtcg tccggatcga gctccttgtg
     1261 gagcaggagg aagcgccgga tctgcgcgcc gcggagcgcc tcgtcctcga gcaggctccg
     1321 gttgacccgg acgacttcct gccggatcag ctcgtacacc tcgggcttct gggcgagatc
     1381 ggtgtagctc gtataggcga gcccgcgccg ctcggcccag ttgccgaccg agaccaggtc
     1441 gatgttgagc agggcggcca cgaacggacg gccgtcaccc acgcacaccg cctccttgat
     1501 gtagggcgag aacttcagct tgttctcgag atacttgggc gcgaagatcg tgccccccgc
     1561 caggcggccc acgtcgcggg cccggtcgat gatcttgagg tggccgtcct tgtcgaagaa
     1621 tccggcgtcg cccgagtgga cccagccctc gacgagggtc tcccgggtgg cgtccgggtt
     1681 cttgtagtag ccctggaaga cccccgggct ccggtacagc acttccccgc tcgggctgac
     1741 gcgcacctcc accccgggca ggggcggccc caccgtgtcg agcttgacgt cgccgtcctg
     1801 ctggatgcag atcagcgcgc tcgactcggt catgccgtag agctgcttca cgttgacgcc
     1861 gagcgagcga tagaactcga agagctcggg cccgatcgcc tcgcccgcgg tgtaggccac
     1921 gcggatcttg cccatgccca ggttgtcgcg cagggggccg tagacgagca gccagccgag
     1981 cgggtagagc agccggtcga gcagcgcggg cgcccgatgc gc
//
LOCUS       DATMCV010001102         3718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_527874, whole genome shotgun sequence.
ACCESSION   DATMCV010001102 DATMCV010000000
VERSION     DATMCV010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527874"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VNU03_25225"
     CDS             complement(<1..1461)
                     /locus_tag="VNU03_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWP77848.1"
                     /translation="MDVLVETRRGRVRGLAEAGLAVYRGIPYARPPVGPRRFGPPEPP
                     EAWTGTHDATRFGSSAPQNGALIGPIMSLGIGRTSEDCLYLNIWTPAADRRRRPVLVW
                     IHGGAFLLGSGSQMLYDGATLARRGDVVVVTINYRLGAFGFLRLRDRFGQRLPATGNE
                     GLLDQVAALTWVRDEIEAFGGDPGQVTIFGESAGSMSCATLLGLPRAHGLFHRAILQS
                     GGANFVWPREIAAELSDHVLDELAVASPEALQTLGAERILEVQRRFFGDLLLGEDHVL
                     GGLSATGQRVAGAMFLGLSLAQRRFGRLTAPLARGLVDVLKRRGRRRPADTMAALRRL
                     RTRGLPFQPVVDGEVLPGHPLVAIEAGAARHVPLLIGTNLDESKLFAPLDPEAATLDE
                     AGLVARCEEAIAAGRVGVGGAGRRAVEVYRAARAARGERVEPGELWFAIETDRTMRHP
                     AMNLAARQAAHQPQTYAYLFTWPSPVLGGMLGSCHAL"
     gene            1484..2065
                     /locus_tag="VNU03_25230"
     CDS             1484..2065
                     /locus_tag="VNU03_25230"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP77849.1"
                     /translation="MSDAPPSPARALPRTVQLKDGAPVVLRLMTPADAAPIAAFARSL
                     PEDDLLFLRMDITDPDAVAHWMRNLEAGLTTTVLAEAGGEIAGYAALVQNRVAWQRHL
                     GEIRTQVALRFRAQGLGRALAGEIFGLARGRGLRKIVAQMTPDQKGAMVTFERLGFRP
                     EALLQDFVVDHAGRTRDLVVMGYDVTGLTDHDD"
     gene            2071..>3718
                     /locus_tag="VNU03_25235"
     CDS             2071..>3718
                     /locus_tag="VNU03_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWP77850.1"
                     /translation="MAPKNLLDPIPHPPRTLVLGNLLSLGISTPIQDMMTLARQYGPI
                     YWLDMMGKPLVVVSGFALVDELCDETRFDKSVKGALRRLRAFAGDGLFTAYTQEPNWS
                     RAHNILLPNFGSRAMQGYHPMMVDIAQQLVLKWERLNVDDDIDVTHDMTSLTLDTIGL
                     CGFGYRFNSFYRDAHHPFVGAMIGALGASMETRGLPLESLIKKDRERQLKADIRYMNE
                     MVDRIIQERRQERRDNASKPDLLSCMLSGVDRKSGERLEDLNIRYQVITFLIAGHETT
                     SGLLSFATYFLLQNPEVLARAYEEVDRVLGSDPSVPPTYAQVNQLTYVGQVLKESLRL
                     WPTAPVFALSPYKDTTIGGTYRMKKPYQIVVLTPMLHRDPAVWGERAEVFDPDNFSRE
                     AEQERPANAYKPFGNGQRACIGRQFALVEATLVLGMILQRFQLVDHARYRLALKETLT
                     VKPDGFRIRVRRRPDRDRRHPPGRPAVRATAAAAAPALPSAGPRHGTPLLVLYGSNLG
                     TAEEVARRVAESGEAGGFAVTVAPLDDYAGRLPGEGAVVIT"
BASE COUNT          552 a         1362 c         1309 g          495 t
ORIGIN      
        1 cagggcgtgg caggagccga gcatgccccc cagcaccgga gagggccagg tgaacaggta
       61 ggcgtaggtc tgcggctgat gcgcggcctg ccgggcggcc aggttcatcg cgggatggcg
      121 catggtccgg tcggtctcga tcgcgaacca cagctccccc ggctcaacgc gctcgccgcg
      181 cgccgcccgc gccgcgcgat agacctcgac cgcgcgtcgc ccggctcccc cgacgccgac
      241 ccgcccggcc gcgatggcct cctcgcagcg ggccaccagg cccgcctcgt cgagggtcgc
      301 ggcctccgga tcgagcggcg cgaagagctt cgactcgtcg aggttggtgc cgatcagcag
      361 cgggacgtgg cgggccgcgc cggcctcgat ggcgacgagc gggtggcccg ggagcacctc
      421 gccatccacc accggctgga aggggaggcc ccgggtgcgc agccgtcgca gcgccgccat
      481 cgtgtccgca ggtcgacgac ggccccgtcg cttgaggaca tcgacgagcc cgcgggcaag
      541 gggcgcggtg aggcggccga accggcgctg cgcgagcgac aggccgagga acatcgcgcc
      601 ggccacccgc tgcccggtgg cggagaggcc gcccagcacg tgatcctcgc ccaggagcaa
      661 gtccccgaag aaccggcgct ggacctcgag gatccgctcg gcgcccagcg tctgcagcgc
      721 ctccggcgag gcgaccgcaa gctcgtcgag cacatggtcg gagagctcgg cggcgatctc
      781 gcggggccac acgaagttgg cgccgccgga ctggaggatc gcgcggtgga agagcccgtg
      841 ggcgcgcggg aggccaagga gggtggcgca gctcatggag ccggccgact cgccgaagat
      901 cgtgacctgg ccggggtcgc ccccgaaggc ctcgatctcg tcgcgcaccc aggtgagcgc
      961 ggccacctgg tcgaggagac cctcgttccc ggtggcgggc agccgctggc cgaagcggtc
     1021 gcgcaggcgc aggaagccga aggcgccgag ccggtagttg atggtgacca ccaccacgtc
     1081 tccccgccgg gccagcgtcg cgccgtcgta gagcatctgg gagcccgagc cgagcaggaa
     1141 ggcgccgccg tggatccaca ccaggaccgg ccgccgccgg cggtcggccg cgggcgtcca
     1201 gatgttcaga tagaggcagt cctcgctggt acggccgatg cccaggctca tgatgggccc
     1261 gatgagcgcg ccgttctgcg gggccgacga tccgaagcgc gtggcgtcgt gcgtgccggt
     1321 ccacgcctcg ggcggctcgg gcggcccgaa gcgccgcggc ccgaccggcg ggcgggcgta
     1381 ggggatcccg cggtacaccg cgaggcctgc ctcggccagg ccgcgcacgc ggccccggcg
     1441 ggtctcgacc aggacgtcca tggcctctat actacgtctc cccatgagcg acgcaccgcc
     1501 gtctcctgct cgagccctcc cgcgcaccgt ccagctcaag gacggcgcgc cggtcgtgct
     1561 ccgcctgatg accccggcgg acgccgcgcc gatcgccgcg ttcgcgcgca gcctgcccga
     1621 ggacgacctg ctgttcctcc gcatggacat caccgatccc gacgcggttg cgcactggat
     1681 gcgcaacctg gaggccgggc tcacgaccac cgttctcgcg gaggcgggcg gggagatcgc
     1741 gggctacgcg gccctggtgc agaaccgggt cgcctggcag cggcacctgg gcgagatccg
     1801 gacccaggtc gcgctccgct tccgcgccca gggcctgggc cgggcgctcg cgggcgagat
     1861 cttcgggctg gcccgcgggc gcggcctccg caagatcgtg gcccagatga cgccggacca
     1921 gaagggggcg atggtcacct tcgagcggct cggcttccgg ccggaggcgc tgctgcagga
     1981 cttcgtggtc gaccacgccg gccgcacccg cgacctcgtg gtgatgggat acgacgtcac
     2041 cggcctgacc gatcacgacg actagcgtcg atggccccga agaacctcct cgatccgatc
     2101 ccgcatcccc cgcgcacgct cgtcctgggc aacctgctct cgctcgggat cagcaccccg
     2161 atccaggaca tgatgacgct cgcgcgccag tacgggccca tctactggct cgacatgatg
     2221 ggcaagccgc tggtggtggt ctcgggcttc gcgctggtcg acgagctctg cgacgagacg
     2281 cgcttcgaca agagcgtcaa gggcgccctg cgccggctcc gcgcctttgc gggcgacggc
     2341 ctcttcaccg cctacaccca ggagccgaac tggtcgcggg cccacaacat cctcctgccc
     2401 aacttcgggt cgcgcgcgat gcagggctat cacccgatga tggtcgacat cgcccagcag
     2461 ctggtgctca agtgggagcg gctcaacgtc gacgacgaca tcgacgtcac tcacgacatg
     2521 acgtcgctca ccctcgacac catcgggctc tgcgggttcg gctaccgctt caactcgttc
     2581 taccgcgacg cccaccaccc gttcgtgggg gcgatgatcg gggccctcgg ggcctcgatg
     2641 gagacgcggg gcctgccgct cgagagcctg atcaagaagg accgggagcg ccagctcaag
     2701 gccgacatcc gctacatgaa cgagatggtg gaccggatca tccaggagcg tcggcaggag
     2761 cgccgggata acgcctcgaa gccggatctg ctgagctgca tgctctctgg cgtggaccgc
     2821 aagagcggcg agcggctcga ggacctcaac atccgctacc aggtgatcac cttcctgatc
     2881 gcggggcacg agacgacgag cggcctcctg tccttcgcca cctacttcct gctccagaat
     2941 ccggaggtgc tcgcgcgcgc ctacgaggag gtggaccggg tcctcggctc cgacccctcg
     3001 gtgccgccga cctacgccca ggtcaaccag ctgacctacg tcggtcaggt gctgaaggaa
     3061 tcactgcgcc tctggcccac cgcgccggtc ttcgccctct cgccctacaa ggacaccacg
     3121 atcggcggga cgtaccggat gaagaagcca taccagatcg tggtactcac acccatgctc
     3181 catcgcgatc ccgcggtgtg gggcgagcgc gccgaggtct tcgatccgga caacttctcg
     3241 cgcgaggccg agcaggagcg cccggccaac gcctacaagc cgttcggcaa cggccagcgg
     3301 gcctgcatcg gccgccagtt cgctctcgtc gaggcgacgc tcgtgcttgg catgatcctg
     3361 cagcggttcc agctggtgga ccacgcccgc taccggctgg ccctcaagga gacgctgacc
     3421 gtcaagccgg acggcttccg catccgggtg cggcggcgac ccgatcgcga ccggcgtcac
     3481 ccgcccggac gtcccgcggt ccgcgcgacg gccgccgcgg cggctccggc cctcccgtcg
     3541 gcgggtccgc ggcacggcac gcccctcctg gtgctctacg gctccaacct gggcaccgcc
     3601 gaggaggtcg cgcgccgggt ggcggagtcg ggtgaggcgg gcggcttcgc ggtcacggtg
     3661 gctccactcg acgactacgc cggtcgcctg cccggcgagg gcgcggtggt catcacgt
//
LOCUS       DATMCV010001103         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_59137, whole genome shotgun sequence.
ACCESSION   DATMCV010001103 DATMCV010000000
VERSION     DATMCV010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59137"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VNU03_25240"
     CDS             complement(<1..834)
                     /locus_tag="VNU03_25240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77851.1"
                     /translation="MKIRWHVQKEQRFPFGSVSPDPDWFEPIGFPPPWPDRPWLYAVM
                     VASANGVVAWPRSGPDDDPVLAILGGDDARDERIADRRHMRHLRCFGDVAIGAQTLRE
                     QPRLIQTPREPGEPPMPALYRFRLAHGLTTEPRHVIYSLRGCLDPALPVFDTPGVEVI
                     VLTTDAGAADLRACGIAAKGAELMVEPLAEPEALRRAHARLFAERGVRYLDCEGGQTV
                     LRALRRAELLDEVFVTETDVVVDESRHRGVLKIFDFEREGADLIAEGRTGDDSPWRFR
                     RW"
     gene            complement(842..1948)
                     /locus_tag="VNU03_25245"
     CDS             complement(842..1948)
                     /locus_tag="VNU03_25245"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HWP77852.1"
                     /translation="MAKAKASKTGVVEKKLISVADLVITAAEKSKDPTLAIPVRSLAN
                     VNFNEKRGIIELGKRKQARTFFNVGMAKKFMQTVLVADALSELQRANLTTSLREIYYR
                     TKHTMKDSSENTFDNQNESDPLIEDLEVTLAALREELHVRAENAGSVVGPLVLDDDGD
                     RVDCARLGKGGYSVPSIVEPEYIAIRRCTADFVLLVEKGTQWNRLSEDKFWRRYNCVL
                     LTGNGQPPRGVRRLARRLHEEYKIPVYVLVDNDPWGYYIYSVVKQGSINLAFESERMA
                     IPKAKFIGLSSADPDRYGLPRNVGIKLNDKDVGRAKELVKYRWFQKKPWQEEIKRMLA
                     SGLKYELDALANKDFRYLTKTYLPRKLKEEDWLD"
BASE COUNT          275 a          679 c          703 g          350 t
ORIGIN      
        1 ccagcgccga aagcgccagg gactgtcgtc gccggtgcgg ccttccgcga tcaggtcggc
       61 gccctcgcgc tcgaagtcga agatcttgag cacgccccgg tgtcgtgatt cgtccaccac
      121 gacgtcggtc tcggtgacga acacctcgtc gagcagctcc gcccgtcgca gggcccgcaa
      181 gaccgtctgg cccccctcgc agtcgagata acggacgccg cgctccgcga agagccgggc
      241 gtgggcccgg cggagcgcct ccggctccgc gagcggctcg accatcagct ccgccccctt
      301 ggccgcgatg ccgcacgcgc gcaggtcggc cgcgccggcg tcggtggtca gcacgatcac
      361 ctcgacgccc ggcgtgtcga agacggggag cgcggggtcg aggcagccgc ggagcgaata
      421 gatgacgtgg cgcggctccg tcgtgagccc gtgggccagg cggaagcgat agagcgcggg
      481 catcggcggc tcgccgggct cgcggggcgt ctggatgagg cggggctgct cgcggagcgt
      541 ctgcgcgccg atcgcgacgt ccccgaagca gcggaggtgc cgcatgtgcc ggcggtccgc
      601 gatgcgctcg tcgcgggcgt cgtctccgcc caggatggcg agcacggggt catcgtcggg
      661 ccccgaccgc ggccaggcca ccaccccgtt ggcggaggcc accatgaccg cgtagagcca
      721 cggccgatcg ggccagggag gcgggaagcc gatcggctcg aaccagtcgg gatcgggtga
      781 gaccgatccg aacgggaagc gctgctcctt ctggacgtgc caccggatct tcacgacccg
      841 gctagtccag ccagtcctct tccttgagct tccgcggcag gtaggtcttg gtcaggtagc
      901 ggaagtcctt gttggcgagc gcgtccagct cgtacttgag gccggaggcg agcatgcgct
      961 tgatctcttc ctgccagggc ttcttctgga accagcggta cttgaccagc tccttggcgc
     1021 ggcccacgtc cttgtcgttg agcttgatgc ccacgttgcg gggcaggccg tagcgatccg
     1081 gatcggcgct ggagaggccg atgaacttgg ccttggggat ggccatccgc tcgctctcga
     1141 acgcgaggtt gatcgacccc tgcttcacca ccgagtagat gtagtagccc caggggtcgt
     1201 tgtcgacgag gacgtagacc gggatcttgt actcctcgtg caggcggcgc gcgaggcgcc
     1261 ggacgccgcg gggcggctgg ccgttgccgg tcaggagcac gcagttgtac cggcgccaga
     1321 acttgtcctc cgagaggcgg ttccactggg tgcccttctc gaccaggagg acgaagtcgg
     1381 cggtgcagcg ccggatcgcg atgtactcgg gctcgacgat cgaggggacc gagtagccgc
     1441 ccttgcccag gcgcgcgcag tccacccggt cgccgtcgtc gtcgagcacc agcgggccca
     1501 ccacgctgcc cgcgttctcc gcgcgcacgt gcagctcctc gcgcagcgcg gccagggtga
     1561 cctcgagatc ctcgatgagc ggatcggact cgttctggtt gtcgaaggtg ttctcggacg
     1621 agtccttcat ggtgtgcttg gtgcggtagt agatctcgcg cagcgaggtc gtcaggttcg
     1681 cccgctgcag ctcgctgagc gcgtcggcca ccagcaccgt ctgcatgaac ttcttggcca
     1741 tccccacgtt gaagaacgtg cgggcctgct tgcgcttgcc cagctcgatg atcccgcgct
     1801 tctcgttgaa gttgacgttg gcgaggctcc ggaccgggat cgccagcgtg gggtccttgc
     1861 tcttctccgc cgcggtgatc acgaggtcgg ccacgctgat gagcttcttc tcgaccacgc
     1921 cggtcttgct cgcctttgcc ttggccatgg gctcgatcct accgcgttct gcgcttcggg
     1981 gccttcgccg ccttgccctt cgcggtc
//
LOCUS       DATMCV010001104         6028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_130806, whole genome shotgun sequence.
ACCESSION   DATMCV010001104 DATMCV010000000
VERSION     DATMCV010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130806"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VNU03_25250"
     CDS             complement(<1..1227)
                     /locus_tag="VNU03_25250"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWP77853.1"
                     /translation="MHRSIHRALRQALITIVLLVGTIGTAAPPAVAQAGRVTGAVHDA
                     LGRPLGGARVRLESSDGKVAGQTTADAQGAFSFSNVAPGSYVVVGEKDGFESATSVVT
                     VTETGQASAELALSSRQALDLAVEAKRLEEARISIQPRIGASTFEFNRQAIEALPQGD
                     NAPLTQLILQAPGVTQDSTGGGFLHVRNEHANVQYRINGIALPDGVSFFGQGGGLSPR
                     LASSVELITGALPAEFGLRTAGIVDVNTKSGAFEPGGYVGLYGGSHSWIQPSAEYRGS
                     LGRFNYYLSGDFLHNNIGISPATPDAPIHDSTKQGHGFGYFEYLIDSTSKVSAILGTF
                     VGHFQIPNAKDQTPSFTVNGVDSFDSAKANETQLEQNYYAVLSYLKAEKELSYQISAF
                     SRYSLLTFRPDPLADLL"
     gene            complement(1318..1614)
                     /locus_tag="VNU03_25255"
     CDS             complement(1318..1614)
                     /locus_tag="VNU03_25255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77854.1"
                     /translation="MSSVFRSRLLQLLLVVPTFLFLVATVQPLHLHKADQPGFYNGEC
                     PLAELAAPGGKVTASPPSFHLWVGLEAGAALLASNDDASAAPAVATDSRAPPLS"
     gene            complement(1679..2158)
                     /locus_tag="VNU03_25260"
     CDS             complement(1679..2158)
                     /locus_tag="VNU03_25260"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HWP77855.1"
                     /translation="MTPMRVIEVSTCRQTGQPLVLLQAEGRDRWLAFYVPMNEANRLA
                     RCLGMTSCPCTPIFELAEQLVGHMGAAVVQAYLEGDERGISAGLVLERDGLELALPCH
                     PADALALAVRARAPILAQDSALALARPAPPSTPDAGDHVRQWLDRVSPGDFEAGTAR"
     gene            complement(2252..2656)
                     /locus_tag="VNU03_25265"
     CDS             complement(2252..2656)
                     /locus_tag="VNU03_25265"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWP77856.1"
                     /translation="MKHGVVPGLALAFVLALAPAARAAHGGTPSVAFTEPDYLRWLID
                     SRQPVVVIDLRPRAEYAAGHLPGALSVPITELDRRFGEIPTTPMVVLYCRCPVEEAAT
                     AYAFLETRGYLNHVVLQEGFEGWTRRRFPIVK"
     gene            complement(2701..3075)
                     /locus_tag="VNU03_25270"
     CDS             complement(2701..3075)
                     /locus_tag="VNU03_25270"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HWP77857.1"
                     /translation="MRLWRVVLLVNLSLGLGLLLGYLAWGRETARLAGELEIARRQQV
                     VAGVERTWQVRGVVRAVLPELNVVVLTHEDLVGFMGSMTMGFRVHDPKLYEGLDIGDR
                     VRFTLTGVPPNVEITALAKEAP"
     gene            complement(3083..5098)
                     /locus_tag="VNU03_25275"
     CDS             complement(3083..5098)
                     /locus_tag="VNU03_25275"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWP77858.1"
                     /translation="MIALGVAARWLHLAAGLGLVGLVTATLLVGHRDRPTVLAWEARM
                     LRWARALTALLLGTGLVTLAHQSAVVTGRPGAALEIGEWTRLLGHSQFGTVWLVRHGV
                     LLLLAALLLLREREESVADRLALRGEAWALAGVGAAAMAWAGHAAAVEGVGLPAALVD
                     AVHLLAAGAWLGALVPLAALLRAAARAEGADARPFAVLAVRAFSRLAFTVMLLLVATG
                     LANTWFQVGSVPALVGTSYGWLLLAKVSLLLPVLLLAQHNRRRLLPRLAGDGDTVGRP
                     AMARLGSFVAAEAGLGLLILLITTGLSLSPPAVHDPVWWPFSQRYAWDVAAALPGGPT
                     RVLIGAQLAFLGLLAMVVGLMLRSWRVILVGLGGLAVVAGLWVALPPMTVDAYPTTFR
                     RSSVPYHVVSVASGAALFRTHCATCHGAGGRGDGAGGAGLPRPPADLTAPHTAQHTAG
                     DMFWWLTHGIPAGGMPAFDTVLSEEERWDLINFIRTLAAGEQARQMTALVPPGRPWLV
                     APDLAIVVGPAPPRSLKDLRDRWMTLLVVFTLPESRARLVQLAEAYNTLQALGTEIVA
                     VPRGDGSDIIRRLGGRPPILYPVITDGAADIATTYTLLARGFGPPPRSAPPLHAEFLI
                     DRQGYIRARWLPGAPGPGWDTPPTLIEQIQLIDKETPAGPAPDEHVH"
     gene            complement(5095..5262)
                     /locus_tag="VNU03_25280"
     CDS             complement(5095..5262)
                     /locus_tag="VNU03_25280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77859.1"
                     /translation="MNAAWLLLTANAWADHGGPLRAESLSPLMVGLLSALLALVAGVL
                     ILVIVMRLTRR"
     gene            5287..5967
                     /locus_tag="VNU03_25285"
     CDS             5287..5967
                     /locus_tag="VNU03_25285"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWP77860.1"
                     /translation="MRAVIFDAGNTLLRMNYRIIAEQLTARGRPVSAEQVEEAELRAR
                     VRLDPHLAPGSSTESTTTHGRYLRYLLEHLAVTDEAEIEAIARWRRGYNLPVGLWNRA
                     DPEALEAVRRVRAAGLVAGVISNSNGSVRAILEETGLAAYLDFIIDSSVVGVEKPDPR
                     IFELGLREAGVGAADAVYVGDLYSVDVLGARGAGLDGILLDPGGFWGPRDCRLVRGLG
                     EAVRLALG"
BASE COUNT          904 a         2052 c         2305 g          767 t
ORIGIN      
        1 gaggagatcg gcgagcgggt cgggacggaa ggtcaggagg ctgtagcggg agaaagccga
       61 gatctggtag gagagctcct tctcggcctt gagatacgag aggaccgcgt agtagttctg
      121 ctcgagctga gtctcgttcg ccttcgcgga atcgaagctg tcgacgccgt tgacggtgaa
      181 gctcggagtc tggtccttcg cgttcgggat ctggaagtgg ccgacgaagg tgccgaggat
      241 cgcgctgacc ttgctggtcg agtcgatcag gtactcgaag tagccgaagc cgtgcccctg
      301 cttggtcgag tcgtggatcg gggcatccgg cgtggccggc gagatgccga tgttgttgtg
      361 caggaagtcg cccgacaggt aatagttgaa ccggccgagc gagccgcgat actccgcgct
      421 cggctggatc cacgagtggc tgcccccgta cagtccgacg tagccgcccg gctcgaaggc
      481 gccgctcttg gtattcacgt ccacgatgcc cgcggtgcgg aggccgaact ccgccggcag
      541 agcaccggtg atgagctcca ccgagctggc cagccgcggg ctcaggccgc cgccctgccc
      601 gaagaagctg acgccgtccg gcagcgcgat gccattgatg cggtactgga cgttcgcgtg
      661 ctcgttgcgc acgtgcagga agccgccgcc ggtggagtcc tgcgttaccc ccggcgcctg
      721 caagatgagc tgggtcagtg gggcgttgtc gccctgaggc agggcctcga tggcctgccg
      781 gttgaactcg aaggtcgagg cgccgatgcg cggctggatg ctgatccgcg cctcctccag
      841 ccgtttcgcc tcgaccgcca ggtccagcgc ttgccgggag gacagagcca gctcggcgct
      901 cgcctggccg gtctcggtga ccgtcaccac cgaggtagca ctctcgaatc cgtccttttc
      961 cccgaccacg acgtaggagc cgggggccac gttcgagaag gaaaacgcgc cctgggcgtc
     1021 ggcggtggtc tggccggcca ccttgccgtc gctgctctcg agtcgaaccc gtgcgccgcc
     1081 cagcgggcgt ccgagggcat cgtgtacggc gccggtgacc cggcccgcct gggccacggc
     1141 gggtggggcc gcggtgccga tcgtaccgac gagcaggacg atggtgatga gggcctgccg
     1201 cagggcccgg tgaatggatc ggtgcatgat cgtgctcctc ccgatggatg acaggtcgca
     1261 tgagggcgcg gagcgcccgg gggcgaagcg atggctacag cggcgaccgg ggtcagatca
     1321 ggagagggga ggagcgcggg aatcggtagc gacagcgggt gcggcggagg cgtcgtcgtt
     1381 cgaggccagg agcgcggcgc cggcttcgag acccacccag aggtggaacg atgggggcga
     1441 cgcggtgacc ttgccgcccg gggcggccag ctcggcgagg gggcactcac cgttgtagaa
     1501 gcccggctga tccgccttgt gcaggtggag cggctgcacc gtcgcgacga ggaacaggaa
     1561 cgtcgggacg acgagcagga gctgcaggag acgcgaccga aagaccgaac tcatggctgg
     1621 gcgctattta tacgggagac ccgcagtctt tgcaacttcc ggcggcgccc ggccgggatc
     1681 aacgggcggt tccggcctcg aagtcgcccg ggctgacccg gtcgagccat tgccgcacgt
     1741 ggtctcccgc gtcgggagta gagggtggcg ccggtcgcgc gagggccagc gcgctgtcct
     1801 gcgcgaggat cggcgcccgc gcccggaccg cgagggcgag cgcgtcggcg gggtggcagg
     1861 gcagcgccag ctcgagcccg tcgcgctcga gcacgaggcc cgccgagatg ccccgctcgt
     1921 caccctcgag ataggcctgc accaccgcgg cgcccatgtg gcccacgagc tgctcggcca
     1981 gctcgaagat cggcgtgcag ggacacgagg tcatgcccag gcagcgggcc agccggttcg
     2041 cctcgttcat gggcacgtag aaggcgagcc agcgatcgcg cccctcggcc tgcagcagca
     2101 ccaggggctg gccggtctgc cgacacgtgc tcacctcgat cacccgcatc ggcgtcatcg
     2161 gcgcgtcctc ccggtcccgg cccgcgatgg ggcatgaggg ttagctacag tgaaacatta
     2221 ggtaacccta atctaccgga gagtcaagcg gctacttcac gatggggaag cggcggcggg
     2281 tccagccctc gaagccctcc tgcagcacga cgtggttcag gtagccgcgc gtctcgagga
     2341 acgcgtaggc ggtggccgcc tcctcgaccg ggcagcggca gtagagcacc accatcgggg
     2401 tggtggggat ctcgccgaag cgccggtcca gctcggtgat cgggaccgag agcgcacccg
     2461 gcaggtgccc ggccgcatac tcggcgcgcg ggcggaggtc gatcaccacc accggctggc
     2521 gcgagtcgat cagccaccgg aggtagtcgg gctcggtgaa ggccacgctc ggcgtgccgc
     2581 cgtgtgcggc gcgggcggcg ggggcgagcg cgagcacgaa ggcgagcgcg aggcccggca
     2641 ccaccccgtg cttcacgggg cctctttggc gcccggctct cgaccgtgat ggccccgttt
     2701 tcacggagcc tctttggcga gcgcggtgat ctccacgttg ggcggcaccc cggtgagcgt
     2761 gaagcggacg cgatcgccga tgtcgaggcc ctcgtacagc ttgggatcgt ggacgcggaa
     2821 gcccatggtc atcgagccca tgaacccgac caggtcctcg tgggtgagca ccaccacgtt
     2881 cagctcgggc agcaccgcgc gcaccacgcc gcgcacctgc caggttcgct cgacgccggc
     2941 gaccacctgc tgccggcgcg cgatctccag ctccccggcc agccgcgcgg tctcccggcc
     3001 ccacgcgaga tagcccagca ggagtcccag gccgagggac aggttgacga gcaggacgac
     3061 ccgccagagg cgcatggcgg ccctagtgta cgtgctcgtc gggcgcgggc ccggcgggcg
     3121 tttccttgtc gatgagctgg atctgctcga tgagggtcgg cggcgtgtcc cagcccgggc
     3181 ccggggcgcc cggcagccag cgggcgcgga tgtagccctg acggtcgatg aggaactccg
     3241 cgtggagcgg cggggcgctg cgcggcgggg ggccgaagcc ccgcgcgagc agcgtgtagg
     3301 ttgtcgcgat gtcggccgcg ccgtcggtga tcaccgggta gaggatcggc gggcggccgc
     3361 ccaggcggcg gatgatgtcg ctcccgtcgc cccgcggcac cgccacgatc tcggtgccga
     3421 gggcctggag cgtgttgtag gcctcggcca gctgcacgag ccgcgcgcgc gactccggca
     3481 gcgtgaagac caccagcagg gtcatccagc gatcgcgcag gtccttgagg ctccgcggcg
     3541 gggcgggacc gaccacgatc gccaggtccg gggccacgag ccacggtcgg ccgggcggca
     3601 cgagcgcggt catctgccgg gcctgctcgc cggccgcgag cgtgcggatg aagttgatca
     3661 ggtcccagcg ctcctcctcg gagagcacgg tgtcgaacgc gggcatgccg ccggcgggaa
     3721 tgccgtgggt gagccaccag aacatgtcgc ccgcggtgtg ctgggcggtg tgcggcgcgg
     3781 tcaggtccgc gggcggccgc ggcaatcccg cgccgcctgc accgtcgccg cgtccgcccg
     3841 cgccgtggca ggtcgcgcag tgggtgcgga agagcgcggc gccgctcgcc accgagacca
     3901 cgtgatacgg gacggacgag cggcgaaaag tcgtgggata agcgtccacc gtcatcggcg
     3961 gcagcgcgac ccagaggccg gcgacgaccg cgaggccgcc caggccgacc aggatcaccc
     4021 gccaggagcg gagcatgagg cccaccacca tcgccaggag gcccaggaac gcgagctgtg
     4081 cgccgatcag cacgcgcgtc ggaccgcccg gcagcgcggc ggcgacgtcc caggcgtagc
     4141 gctgcgagaa cggccaccag accggatcgt gcaccgcggg cggcgagagc gagagcccgg
     4201 tcgtgatgag gaggatcagc agcccgagcc cggcctccgc ggccacgaag gatccgaggc
     4261 gcgccatcgc gggccgtccc accgtgtcgc cgtcgcccgc gagccggggc aggagccggc
     4321 gccggttgtg ctgggcgagc aggagcaccg ggagcagcag ggagaccttc gcgagcagga
     4381 gccacccgta ggacgtgccg accagcgcgg gcacgctgcc cacctggaac caggtattgg
     4441 cgagaccggt ggcgacgagc aggagcatga ccgtgaaggc caggcgcgag aacgcccgca
     4501 ccgcgagcac cgcgaagggt cgcgcatccg ccccctcggc gcgtgccgcg gcccgcagca
     4561 gcgcggcaag cggcacgagc gctcccagcc acgcgccggc ggccaggaga tgcacggcgt
     4621 cgaccagcgc ggccgggagc ccgacgccct cgaccgcggc ggcgtgcccg gcccacgcca
     4681 tggccgcggc gccgacgccc gcgagcgccc aggcctcgcc gcgcagggcg agccggtcgg
     4741 cgacggactc ctcgcgctcc cgcagcagga gcagcgcggc caggaggagc agcacgccgt
     4801 ggcgcaccag ccacaccgtg ccgaactggc tgtggccgag gagccgcgtc cactcgccga
     4861 tctcgagcgc ggcgcccggc cgtccggtga ccaccgccga ttgatgcgcg agcgtgacga
     4921 gcccggtgcc gagcaggagc gcggtgagcg cgcgcgccca gcggagcatg cgcgcctccc
     4981 aggcgagcac ggtcgggcga tcgcggtgtc ccaccagcag ggtggcggtg accaggccca
     5041 cgagcccgag accggcggcc aggtgcagcc agcgcgccgc cacgccgagc gcgatcatcg
     5101 gcgagtgagc cgcatcacga tgaccaggat cagcacgccc gcgaccaggg ccagcagggc
     5161 ggagaggagg cccaccatca gcgggctcag gctctccgcg cgcagcgggc cgccgtggtc
     5221 ggcccacgcg ttcgcggtga ggaggagcca ggcggcgttc attggcggcc tcattataat
     5281 gagtccgtgc gcgccgtcat cttcgacgcc ggcaacaccc tgctgcggat gaactaccgg
     5341 atcatcgcgg agcagctcac ggcccgcggc cgtccggtga gcgcggagca ggtggaggag
     5401 gccgagctgc gggcccgcgt gcggctggac ccgcacctgg cgccgggtag ctcgaccgag
     5461 agcacgacca cgcacggccg ctacctgcgc tacctgctcg agcatctggc ggtcaccgac
     5521 gaggccgaga tcgaggccat cgcgcgctgg cgccgcgggt acaacctgcc ggtggggctc
     5581 tggaaccggg ccgatccgga ggcgctcgag gcggtgcgcc gggtgcgggc ggcgggcctg
     5641 gtggcgggcg tcatctcgaa ctccaacggc tcggtgcggg ccatcctgga ggagaccggg
     5701 ctcgccgcgt atctcgactt catcatcgac tcctcggtgg tgggcgtgga gaagccggac
     5761 ccgcgcatct tcgagctggg cctgcgggag gccggggtgg gcgccgcgga cgcggtgtac
     5821 gtgggggatc tctactcggt ggacgtgctg ggggcgcgcg gggccgggct cgacgggatc
     5881 ttgctcgacc cggggggctt ctgggggccg cgggactgcc ggctggtgcg tgggctcggg
     5941 gaggcggtgc gcctggcgct ggggtagggt cgctcgccac cgggcccctc aacaccggac
     6001 ccctcaccct gccctctccc cggagggg
//
LOCUS       DATMCV010001105         3789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_59272, whole genome shotgun sequence.
ACCESSION   DATMCV010001105 DATMCV010000000
VERSION     DATMCV010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59272"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VNU03_25290"
     CDS             complement(<1..875)
                     /locus_tag="VNU03_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HWP77861.1"
                     /translation="MKIAILDDYQDTIRTLDCFKKMSGQDVTIWKDHTKDVDTLAGRL
                     KDTEVLVCIRERTPIRGPLLDRLERLKMVTQVGVVPNIEVPACTRRGVIVSSSQMPGR
                     PSYATAELTWGLVVAAVRRIPQEMIAMRAGKWQAYPIGTGLRGKTLGILGYGKIGAVV
                     AGYGRAFGMNVLAWGRPSTLEKAKADGYTPAASREAFFAESDVVSIHVRLIDATRGMV
                     TAEDLARMKPSAVFVNTSRAGLVAPGALEAALAKGRPGMAAVDVYEDEPVIGATHPLL
                     KMDNAICAPHLGYVER"
     gene            952..2064
                     /locus_tag="VNU03_25295"
     CDS             952..2064
                     /locus_tag="VNU03_25295"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP77862.1"
                     /translation="MAETARMAVFHGAGKPFEIREYPVPDPEPGAVLVKVAMANVCGS
                     DLHYWRGELDYVKMGRPLPLNTGHEHVGRVAKLGAGVTTDSAGRPLAVGDRVAYRFFF
                     PCGRCRMCLKGKTKSCPVRQANFLVSCEVWPHFQGGFGQYFYLRPNHAVFKLSDDITD
                     EMAAGVNCAFSQVYAGLDIAAVRAGQTVVIQGAGGLGVYACAVAKEMGAARVVVIDGV
                     EERLTLARSFGATDVVDMREHKTPEDRIKAVKKLTDDWGGDVVMELVGNPKVVDEGLR
                     MTAPEGTYLEIGNINVGWKAEFDPAWIIFGNRRIIGLSHYEAEHLRGALDMMARTRGR
                     YPWDRIISHKFPLEAIEEAFVQQDKGHVTRAAIIPN"
     gene            2083..2574
                     /locus_tag="VNU03_25300"
     CDS             2083..2574
                     /locus_tag="VNU03_25300"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HWP77863.1"
                     /translation="MAGIYDFTVKSIDGTPQSLGDYRGKALLVVNVASKCGLTPQYEA
                     LQALHAEYAPRGFAVLGFPCNQFAGQEPGSDADVKAFCSTSYGVTFPLYSKIEVNGKD
                     RAPLYAWLTGQATGPDGPGDIKWNFAKFLVDKTGQVVARFEPKTAPGAPEVKQAIAKA
                     VGV"
     gene            2600..2752
                     /locus_tag="VNU03_25305"
     CDS             2600..2752
                     /locus_tag="VNU03_25305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77864.1"
                     /translation="MHAFVAADNMIFPPNSKDEAAYRTAGRAKRDMESFLLVRSGVEL
                     SRKRAA"
     gene            2777..>3789
                     /locus_tag="VNU03_25310"
     CDS             2777..>3789
                     /locus_tag="VNU03_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWP77865.1"
                     /translation="MRIHSVEATAIDIPLRRNFGGSTYAVLKRSTVITRLRTADGPVG
                     VVYNGDNREHGTEIVRLIRDELAPILRGQDPAEVERIWSRLFALSHASRDRKLLMEAI
                     ACVDCAIWDVLGRARGQSVCAMLGGRPGRRLPIISIGGYYREGKTLADIGREMELYRE
                     AGMAGCKFKVGGLAPEADAERVQAARAAAGPDFVLAVDANRGFTVEDAVAFARRVEPL
                     GIAWFEEPCHWHDDARMMAEVRRVTRIPVTAGQSEITSHAVRRLLDAGAVDLVNFDAS
                     EGGGVTEWRRAAALCEAASVKMAHHEEPQIAGQLLAAVPHGTYVECFADPERDPAWQA
                     LWA"
BASE COUNT          568 a         1327 c         1345 g          549 t
ORIGIN      
        1 cgctccacgt agcccaggtg aggcgcgcag atcgcgttgt ccatcttgag cagcggatgc
       61 gttgcgccga tcaccggctc gtcctcgtag acgtcgaccg cggccatgcc cggccgcccc
      121 ttggcgagcg cggcctccag cgcgccgggg gccaccagcc ccgcgcggct cgtgttgacg
      181 aagaccgcgg agggcttcat ccgcgcgagg tcctccgcgg tcaccatgcc gcgggtcgcg
      241 tcgatcagcc gcacgtggat cgagaccacg tcggactcgg cgaagaacgc ctcgcggctc
      301 gcggccgggg tgtagccgtc ggccttcgcc ttctcgagcg tggacgggcg cccccacgcg
      361 agcacgttca tcccgaacgc gcggccgtag cccgcgacca ccgcgccgat cttgccgtag
      421 ccgaggatgc cgagggtctt gccgcgcagc ccggtgccga tcggataggc ctgccacttg
      481 cccgcgcgca tcgcgatcat ctcctgcggg atgcgccgca ccgccgcgac caccaggccc
      541 caggtcagct ccgcggtcgc gtacgacggg cggcccggca tctggctcga cgagacgatc
      601 acgccgcgcc gcgtgcacgc ggggacctcg atgttgggaa ccacgccgac ctgggtgacc
      661 atcttcagcc gctcgagacg gtcgagcagc ggcccgcgga tcggggtgcg ctcgcggatg
      721 cacacgagca cctcggtgtc cttgaggcgg cccgcgaggg tatcgacgtc cttggtgtga
      781 tccttccaga tcgtgacatc ctggccggac atcttcttga agcagtcgag cgtgcggatg
      841 gtgtcctggt agtcgtcgag gatcgcgatc ttcatgtgcc gccccccctc gggcgccccg
      901 tagcctacca tgctagactt ccgccgtcgt ccatcatcta aggaggcact catggccgag
      961 acggcgagga tggcggtgtt ccacggcgcg ggcaagccat tcgagatccg cgagtacccg
     1021 gtgccggatc ccgagccggg cgcggtgctg gtcaaggtcg cgatggccaa cgtgtgcggc
     1081 tccgacctcc actactggcg cggcgagctt gactacgtga agatgggccg cccgctgccg
     1141 ctcaacaccg gccacgagca cgtgggccgc gtggccaagc tcggggccgg tgtcaccacc
     1201 gactcggcgg gccgtccgct cgcggtgggc gaccgcgtcg cgtaccgctt cttcttcccg
     1261 tgcgggcgct gccgcatgtg tctcaagggc aagaccaagt cctgtccggt gcgccaggcc
     1321 aacttcctcg tctcctgcga ggtgtggccg cacttccagg gcggcttcgg ccagtacttc
     1381 tacctgcgtc cgaaccacgc ggtgttcaag ctgtcggacg acatcaccga cgagatggcg
     1441 gcgggtgtca actgcgcctt ctcccaggtc tacgccggcc tcgacatcgc ggcggtgcgg
     1501 gcgggccaga cggtggtgat ccagggcgcg ggcggcctcg gggtctacgc gtgcgcggtg
     1561 gcgaaggaga tgggcgcggc gcgcgtcgtc gtcatcgacg gggtggagga gcgtctcacc
     1621 ctggcccgct ccttcggggc caccgacgtg gtcgacatgc gcgagcacaa gacgcccgag
     1681 gatcgcatca aggcggtgaa gaagctcacc gacgactggg gcggcgacgt ggtgatggag
     1741 ctggtgggca accccaaggt ggtcgacgag gggctgcgca tgaccgcgcc ggagggcacc
     1801 tacctcgaga tcggcaacat caacgtgggc tggaaggcgg agttcgatcc ggcgtggatc
     1861 atcttcggca accgccgcat catcggcctc tcccactacg aggccgagca cctgcgcggc
     1921 gcgctcgaca tgatggcccg cacccgcggg cgctacccgt gggaccggat catctcgcac
     1981 aagttccccc tggaggcgat cgaggaggcc ttcgtccagc aggacaaggg tcacgtcacc
     2041 cgcgccgcca tcatccccaa ctgacccgag acggagagcg ccatggctgg gatctacgac
     2101 ttcaccgtga aatcgatcga cggcacgccg cagtccctcg gggactatcg cggcaaggcg
     2161 ctcctggtgg tcaacgtcgc gtcgaagtgc gggctgaccc cgcagtacga ggcgctccag
     2221 gcgctccacg ccgagtacgc cccgcgcggc ttcgcggtgc tgggcttccc gtgcaaccag
     2281 ttcgcgggtc aggagccggg cagcgacgcg gacgtgaaag cgttctgctc gaccagctac
     2341 ggggtgacgt tcccgctgta ctcgaagatc gaggtgaacg gcaaggaccg cgccccgctc
     2401 tacgcctggc tcaccgggca ggccaccggc cccgatggcc ccggcgacat caagtggaac
     2461 ttcgccaagt tcctggtgga caagaccggc caggtggtcg cgcgcttcga gcccaagacc
     2521 gcgcccgggg cgccggaggt caagcaggcc atcgcgaagg cggtcggcgt ctgagccggc
     2581 ccccgcgccg ggagacgcga tgcatgcttt cgtcgccgcc gacaacatga tcttcccgcc
     2641 aaactccaag gacgaggcgg cgtatcggac ggcggggcgg gcgaagcgag acatggagtc
     2701 gttcctgctg gtccgcagcg gcgtcgagct cagccggaag cgcgccgcct gaagcggtcg
     2761 tcgagcgagc cggcgcatgc gcatccactc cgtcgaggcg accgcgatcg acattccgct
     2821 gcggcggaac ttcgggggca gcacctacgc ggtcctcaag cgctccacgg tcatcacccg
     2881 gctccgcacc gccgacggcc ccgtcggagt ggtctacaac ggggacaacc gcgagcacgg
     2941 gaccgagatc gtccggctga tccgggatga gctggcgccg atcctccggg gccaggaccc
     3001 ggccgaggtc gagcggatct ggagccgcct gttcgccctc tctcacgcga gccgcgaccg
     3061 caagctcctg atggaagcga tcgcctgcgt ggactgcgcg atctgggatg tgctcggccg
     3121 ggcgcgcggc cagagcgtgt gcgcgatgct cggcggccgg ccgggccggc ggctgcccat
     3181 catctccatc ggcggctact accgcgaggg caagacgctg gccgacatcg gccgcgagat
     3241 ggagctctac cgcgaggccg gcatggccgg ctgcaagttc aaggtgggcg gcctcgctcc
     3301 cgaggccgac gcggagcgcg tccaggccgc gcgggccgcc gccgggcccg acttcgtcct
     3361 cgcggtggac gccaaccgcg gcttcacggt cgaggacgcg gtcgccttcg cgcggcgggt
     3421 ggagccgctc gggatcgcgt ggttcgagga gccgtgccac tggcacgacg acgctcgcat
     3481 gatggcggag gtgcggcggg tgacgcgcat cccggtcacc gcgggccagt ccgagatcac
     3541 gagccacgcg gtgcggcgac tcctcgacgc gggggcggtc gacctggtga acttcgatgc
     3601 ctccgaaggc ggcggggtca ccgagtggcg gcgggccgcc gcgctctgcg aggccgcctc
     3661 cgtgaagatg gcgcaccacg aggagccgca gatcgcgggc cagctcctgg ccgcggtgcc
     3721 gcacggcacc tacgtcgagt gcttcgccga tccggagcgc gacccggcct ggcaggccct
     3781 ctgggccaa
//
LOCUS       DATMCV010001106         3127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_328877, whole genome shotgun sequence.
ACCESSION   DATMCV010001106 DATMCV010000000
VERSION     DATMCV010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328877"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1455
                     /locus_tag="VNU03_25315"
     CDS             58..1455
                     /locus_tag="VNU03_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWP77866.1"
                     /translation="MSSAAAAGVAIGSAPALSPRTRRLLEGPVAPTLLRLAAPNVLVM
                     VLQAAVSTLDAVFVGWLGSDALAGVSLVFPLVMLMQTMSAGGMGGGVASAVARALGGG
                     RRAEADALVVHALVIAVIMAAAFTLLLELGGPALYHAMGGDGAVLASATLYSRVLFGG
                     ALAYWLFNTLGSVIRGTGNMALPASVMVGSGCLYLALSPALILGWWPLPRLGIAGAAA
                     ASVTSFSLGSLALLAFLLSGRGLVRPTWRGLRLRWPLFREILRVGAPGSLNTILTNLT
                     VVLLTGLVGPFGATALAGYGLGARLEYLQIPIVFGLGSGLVTMVGTSMGAGHRARAQR
                     VAWVGAAMAAGVTGTIGILAALFPHAWLGLFTSDPLVLAAGARYLRIVGPCYGFFGAG
                     LALYFASQGAGRLLWPLLSAVVRLAIAAAGGWLATRWLGTGLSGLFAAMAAALVAMGI
                     INVAAVHFGAWRRRP"
     gene            1452..1955
                     /locus_tag="VNU03_25320"
     CDS             1452..1955
                     /locus_tag="VNU03_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015134135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77867.1"
                     /translation="MTTQQIFGLQVLLSFVLFTLVARWYMSPRLAALPVDRALQPLLV
                     LHATRHMGLVFLVPTVVGGTLPASFAAAAGYGDLLAGVLALAALVALRAPLPRVAMPL
                     TWLFNVAGLLDLVNAFYQGLAHDVQLGAAYYIPTFIVPALVVTHVMMFGLLLRHPASA
                     RLDLPAS"
     gene            1979..>3127
                     /locus_tag="VNU03_25325"
     CDS             1979..>3127
                     /locus_tag="VNU03_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77868.1"
                     /translation="MSRRARAILATACGAHFIHDGFSDILYILLPVWATEFGLSFAQV
                     GLLKTLYTGGMASFQVPAGILAERRGEARLLAAGTAVTALGFLAAGLTGGFLALLVCL
                     AVGGLGSGCQHPLSSSLVSRAYETGPRRTALGTYNFSGDLGKVAVPAAVALALPWLGW
                     RRAVEVYAVVGLLAAAAILALLGRLGAGRPEAPAPASAAAAPRTAGWGIRDPRAYRAL
                     SAIHVIDNSTRTGFLTFLPFLLIAKGATVQSVGFALMLVFIGGAIGKFACGALADRVG
                     VIRTVVITEAATGVGILLLLTLPLGACLLALPPLGIALNGTSSVLYGTVADLVSSDRR
                     ARSYAIFYTLGIGASALSPFAYGLLSDWGGVRLTLTVVGLVVFLTLPFT"
BASE COUNT          279 a         1221 c         1099 g          528 t
ORIGIN      
        1 gcgcgcgcct ccagcacctg catgaccctg cgtggcgagc aggcggtggg ccgctaggtg
       61 agctcggccg ccgcggcggg ggtggcgatc ggctcggcgc ccgcgctgtc gccccgcacc
      121 cgtcggctgc tcgaggggcc ggtggctccg accctgctgc ggctcgccgc gcccaacgtg
      181 ctcgtcatgg tgctgcaggc cgcggtcagc acgctcgacg cggtgttcgt gggctggctc
      241 ggctcggacg cgctcgccgg ggtctcgctc gtcttcccgc tcgtgatgct catgcagacg
      301 atgtccgcgg gcggcatggg cggcggcgtg gcctcggcgg tggcccgggc cctcggcggc
      361 ggccgccgcg ccgaggccga cgcgctggtg gtccacgccc tcgtgatcgc ggtgatcatg
      421 gccgccgcgt tcaccctgct cctcgagctg ggcggccccg cgctctacca cgcgatgggc
      481 ggcgacgggg cggtgctcgc ctcggccacg ctctattccc gggtcctctt cggcggcgcg
      541 ctcgcctact ggctcttcaa caccctcggc agcgtgatcc gcggcaccgg caacatggcg
      601 ctgcccgcgt cggtgatggt gggcagcggg tgcctctacc tcgcgctctc ccccgccctc
      661 atcctgggct ggtggccgct gccgcgcctc ggcatcgcgg gcgcggccgc ggccagcgtc
      721 acctcgttca gcctgggcag cctcgcgctg ctcgcgttcc tgctctccgg acggggcctg
      781 gtgcggccga cctggcgcgg cctgcgcctg cggtggccgc tcttccggga gatcctccgg
      841 gtgggcgcgc cgggctcgct gaacacgatc ctcacgaacc tgaccgtggt gctcctcacc
      901 gggctggtcg ggcccttcgg cgccaccgcg ctggccggct acggcctggg cgcgcggctc
      961 gaatacctgc agatcccgat cgtgttcggt ctgggctcgg ggctcgtcac catggtcggc
     1021 accagcatgg gcgccggcca ccgggcgcgg gcccagcgag tggcgtgggt cggcgcggcg
     1081 atggcggccg gcgtcaccgg cacgatcggg atcctggccg cgctgttccc gcacgcctgg
     1141 ctcggcctgt tcacgagcga cccgctcgtc ctggccgccg gggcgcgcta cctgcgcatc
     1201 gtcgggccct gctacggctt cttcggcgcc ggcctcgccc tgtacttcgc ctcgcagggg
     1261 gcgggccgcc tgctgtggcc gctcctctcc gcggtcgtcc gcctcgcgat cgcggcggcg
     1321 ggcggatggc tcgccacccg gtggctcggg accggccttt ccgggctgtt cgccgcgatg
     1381 gcggcggcgc tggtggcgat ggggatcatc aacgtcgcgg cggtgcactt cggagcgtgg
     1441 cggaggcgtc catgaccacc cagcagatct tcggcctcca ggtgctgctg agcttcgtcc
     1501 tgttcacgct ggtggcgcgg tggtacatga gcccgcgcct tgccgccctg ccggtggacc
     1561 gcgcgctcca gcccctgctg gtgctccacg ccacccgcca catggggctc gtgttcctgg
     1621 tgccgaccgt ggtgggcggc acgctcccgg cgtccttcgc ggccgccgcg ggctacggcg
     1681 atctcctggc cggcgtgctc gccctggccg cactcgtggc cctgcgcgct ccgctgccgc
     1741 gcgtggcgat gccgctgacc tggctcttca acgtggcggg cctgctcgac ctggtcaacg
     1801 ccttctacca ggggctcgcc cacgacgtgc agctcggcgc cgcctactac atccccacct
     1861 tcatcgtgcc ggccctcgtg gtcacgcacg tgatgatgtt cggcctactg ctgcggcacc
     1921 cggcgtccgc ccgcctcgat ttgcccgcgt cgtagcgaat gtgaaacaat cgcgggcgat
     1981 gagtcgccgc gcccgcgcca tcctcgccac cgcctgcggg gcgcacttca tccacgacgg
     2041 gttctccgac atcctctaca tcctgctgcc ggtgtgggcc accgagttcg gcctgagctt
     2101 cgcccaggtg gggctgctca agaccctcta caccggcggg atggcgagct tccaggtgcc
     2161 ggccggcatc ctcgcggagc gccgcggcga ggcgcggctg ctcgcggcgg gcaccgcagt
     2221 gaccgcgctc ggcttcctgg ccgccgggct caccggcggg tttctcgccc tcctcgtctg
     2281 cctcgcggtc ggcgggctcg gctccggctg ccagcacccg ctgtcgtcgt cgctcgtgtc
     2341 acgggcctac gagaccgggc cacggcgcac cgcgctcggc acctacaatt tctccggcga
     2401 cctcggcaag gtcgcggtgc cggccgcggt cgcgctggcg ctgccgtggc tcggctggcg
     2461 gcgcgcggtg gaggtctacg cggtggtcgg cctgctggcc gccgccgcga ttctcgcgct
     2521 gctcggacgc ctgggcgccg gccgcccgga ggcgccggca cccgcgagcg ccgcggccgc
     2581 cccacgcacc gccggctggg gcatccgcga tccgcgcgca taccgcgcgc tctccgcgat
     2641 ccacgtcatc gacaactcga cccgcaccgg cttcctcacc ttcctgccct tcctgctgat
     2701 cgccaagggc gccaccgtgc agtcggtggg gttcgccctg atgctggtct tcatcggcgg
     2761 cgccatcggg aagttcgcgt gcggggccct ggccgaccgc gtgggcgtga tccgcaccgt
     2821 ggtcatcacc gaggccgcga ccggcgtcgg catcctgctg ctcctgaccc tgccgctcgg
     2881 ggcctgcctg ctcgcgctgc cgccgctcgg catcgcgctc aacgggacct cctcggtcct
     2941 ctacggcacg gtggccgacc tggtctcgag cgatcgccgc gcgcggagct acgcgatctt
     3001 ctacacgctc ggcatcggcg cctccgcgct ctcgccgttc gcctacggcc tgctgagcga
     3061 ctggggcggc gtccgcctca ccctcaccgt cgtcggcctc gtcgtcttcc tcacgctgcc
     3121 gttcacg
//
LOCUS       DATMCV010001107         2886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_197757, whole genome shotgun sequence.
ACCESSION   DATMCV010001107 DATMCV010000000
VERSION     DATMCV010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197757"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..822
                     /locus_tag="VNU03_25330"
     CDS             10..822
                     /locus_tag="VNU03_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77869.1"
                     /translation="MPPTSGRARWAGLAQRVVPPAAIGFVALFAWEAVVRLKAIPPYI
                     LPGPLLIGATLLKDWHTLYPSLLVTLAVTGAALLVATVAGTAIAILFAQWHWVERSFF
                     PYAVILQVTPVVAIAPLIIIWVKNIQLSLLICAWIVAFFPVVSNTTLGLNSTDHHLLE
                     LFDLYGASRRQVLWYLRLPGALPYFFGGLRISGGLALIGAVVAEFVAGTGGAQSGLAY
                     RILEAGYTLQIPRMFAALLLVSMTGVVIFLALSALSHLVLRRWHESARAREQ"
     gene            824..2152
                     /locus_tag="VNU03_25335"
     CDS             824..2152
                     /locus_tag="VNU03_25335"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006620071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HWP77870.1"
                     /translation="MPAGFVDLPPSGRYWLRDGRVPTTLLAEPVPELVADAEGLAQVD
                     LEIVGAVVARIAPAGAAPADAAGVRLDGGQVWPCFVDLHTHLDKGHIWQRAPNPDGTF
                     AAALRTVATDRAARWTAEDVSRRMDFGLRCSFAHGTRAIRTHLDSLGPQAAISWPVMR
                     ALRQRWAGRVELQAASIVPVEIFTRPDGEHLADLVANAGGILGGVTYMSPALDAQLDR
                     ALALAAERGLGLDFHVDESGDPGARSLAHIARAALRHRFAGPIVCGHCCSLAMQPPEV
                     VEETLDLVKQAGIAIVTLPMCNLYLQNRDPGRTPHWRGVTLLHEMKTRGIPVAVASDN
                     CRDPFHGYGDHDMLEVFREATRIAHLDRPHGDWPRAVTSTPADLMGLAPVGRLGAGLP
                     ADLVLFRSRHWSELLSRPQSDRVVVRAGRVLDAALPDYRELDDLMTETLR"
     gene            2149..>2886
                     /locus_tag="VNU03_25340"
     CDS             2149..>2886
                     /locus_tag="VNU03_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWP77871.1"
                     /translation="MTLDRLVHALDGVTVITDPNVVRVKSKDFYWYSPVLRRKLDDKC
                     AEIVVCPRSEEEVVRVARACVAAKVPLTVRGAGTGNYGQAVPLEGGVILDMTAMKAVR
                     WIRPGMARVEAGLKLVELDAEARRQGWEIRMFPSTKRTATVGGFIGGGSGGVGSITYG
                     QLRDRGNVHAARVVTMEDEPRVLELRGDDVQKVNHAYGTNGIITELEIPLGPTYPWID
                     SVVVFDDFMDAARFAQILGESDGIVKKL"
BASE COUNT          371 a         1078 c         1008 g          429 t
ORIGIN      
        1 gcggaggccg tgccgcccac ctcgggccgg gcccggtggg ccgggctcgc gcagcgcgtg
       61 gtcccccccg ccgccatcgg cttcgtggcg ctcttcgcct gggaggcggt ggtgcgactc
      121 aaggcgatcc cgccctacat cctgccgggc ccgctgctga tcggcgccac gctcctgaag
      181 gactggcaca cgctctatcc gtcgctcctc gtgaccctgg cggtgaccgg cgcggcgctg
      241 ctggtggcca cggtggccgg caccgcgatc gcgatcctgt tcgcccagtg gcactgggtc
      301 gagcggagct tcttcccgta cgcggtcatc ctccaggtca ccccggtcgt ggccatcgcc
      361 ccgctcatca tcatctgggt gaagaacatc cagctctcgc tcctgatctg cgcgtggatc
      421 gtggcgttct tcccggtggt gtccaacacc acgctcggcc tcaacagcac cgaccaccac
      481 ctgctcgagc tgttcgacct ctacggcgcc tcgcggcgcc aggtgctctg gtacctccgg
      541 ctccccggcg cgctgccgta ctttttcggg ggcctgcgca tcagcggggg cctggccctg
      601 atcggcgcgg tggtggccga gttcgtcgcg ggcacgggcg gcgcccagtc cggactcgcc
      661 tatcgcatcc tcgaggccgg ctacaccctg cagatcccgc gcatgttcgc cgcgctgctc
      721 ctggtctcga tgaccggcgt cgtcatcttc ctggccctct ccgctctgtc gcacctggtc
      781 ttgcggcgct ggcacgagag cgcccgcgcg cgcgagcagt agcgtgccgg ccggattcgt
      841 ggatcttcct ccgagcggcc gctactggct gcgagacggg cgcgtgccga ccaccctgct
      901 ggccgagccg gtgccggagc tcgtggccga cgcggagggc ctggcccagg tcgacctcga
      961 gatcgtgggc gcggtcgtcg cgcgcatagc gcccgcgggg gccgcccccg ccgacgccgc
     1021 gggcgtgcgc ctcgacggcg gccaggtctg gccgtgcttc gtggatctcc acacgcatct
     1081 ggacaagggc cacatctggc aacgcgcccc gaaccccgac ggcaccttcg ccgccgccct
     1141 ccgcaccgtc gcgacggacc gcgccgcgcg ctggaccgcc gaggacgtct cgcgccgcat
     1201 ggacttcggc ctgcgctgca gcttcgcgca cggcacgcgc gcgatccgga cccatctcga
     1261 ctcgctcggc ccgcaggccg cgatcagctg gccggtgatg cgcgcgctcc ggcagcggtg
     1321 ggccgggcgc gtcgagctgc aggcggcctc gatcgtgccg gtcgagatct tcacgcgccc
     1381 cgacggcgag cacctggccg atctggtggc caatgcgggc ggcattctcg gcggagtgac
     1441 ctacatgagc ccggcgctcg acgcccagct cgaccgcgcc ctcgcgctgg ccgccgagcg
     1501 gggcctcggc ctcgacttcc acgtggacga gagcggcgac cccggcgcgc gctcgctcgc
     1561 gcacatcgcc cgggccgcgc tccgccaccg cttcgccggg cccatcgtct gcggccactg
     1621 ctgcagcctg gcgatgcagc cgcccgaggt ggtggaggag acgctcgacc tggtcaagca
     1681 ggccggcatc gcgatcgtca cgctgccgat gtgcaacctg tatctccaga accgcgaccc
     1741 gggacggact ccgcactggc gcggcgtgac cctgctccac gagatgaaga cgcgcggcat
     1801 cccggtggcg gtggcgagcg acaattgccg cgatcccttc cacggctacg gcgaccacga
     1861 catgctggag gtcttccgcg aagccacgcg gatcgcgcac ctcgatcggc cccacgggga
     1921 ctggccccgc gcggtcacga gcacgcccgc ggacctgatg gggctcgcgc cggtcgggcg
     1981 cctcggcgcg ggcctgcccg ccgatctcgt gctcttccgg agccgccact ggagcgagct
     2041 gctctcgcgc ccgcagagcg atcgggtggt ggtgcgcgcg gggcgcgtgc tcgacgcggc
     2101 cctgcccgac taccgcgagc tggacgacct gatgacggag acgctgcgat gaccctcgac
     2161 cgcctggtgc acgcgctcga cggcgtcacc gtcatcaccg accccaacgt ggtgcgggtg
     2221 aagtccaagg acttctactg gtacagcccg gtgctgcggc gcaagctcga cgacaagtgc
     2281 gccgagatcg tggtgtgtcc gcgctccgag gaagaggtcg tccgggtcgc gcgggcctgc
     2341 gtggccgcca aggtgccgct caccgtgcgc ggcgccggta ccgggaacta cggccaggcc
     2401 gtcccgctcg agggcggcgt catcctcgac atgaccgcga tgaaggcggt ccggtggatc
     2461 cgaccgggca tggcgcgggt ggaggccggc ctcaagctgg tcgagctgga cgcggaggcg
     2521 cgtcgccagg gatgggagat ccgcatgttc ccctccacca agcggaccgc caccgtgggc
     2581 ggattcatcg gcgggggaag cggcggggtg ggctcgatca cctacggcca gctccgcgac
     2641 cgcggcaacg tccacgccgc gcgggtggtg acgatggagg acgagccccg cgtcctcgag
     2701 ctgcgcggcg acgacgtgca gaaggtcaac cacgcctacg gcaccaacgg gatcatcacc
     2761 gagctcgaga tcccgctcgg ccccacctac ccgtggatcg actcggtggt ggtcttcgac
     2821 gacttcatgg acgcggcccg cttcgcccag atcctcgggg agtcggacgg catcgtcaag
     2881 aagctg
//
LOCUS       DATMCV010001108         2581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_198038, whole genome shotgun sequence.
ACCESSION   DATMCV010001108 DATMCV010000000
VERSION     DATMCV010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198038"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1085
                     /locus_tag="VNU03_25345"
     CDS             441..1085
                     /locus_tag="VNU03_25345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWP77872.1"
                     /translation="MTKKTRAALVAAQRGHRELRVLRNERNRGKGYSIRRAYAECAGT
                     HVVFTDADLPYGLDSLEAALAALEGGADLVIGSRVLPGSMVRMSSQHFPYIFARHLLG
                     RAMLRAVNLMFGLDVSDTQCGFKACRLSVARYLASRLSIDRFAFDVELILAARRAGYH
                     IAEIPVELNYAGRMTTVRVLRDAPSVLRDLCQIKVADWRGHYEPREADLQYHVE"
     gene            1082..1696
                     /locus_tag="VNU03_25350"
     CDS             1082..1696
                     /locus_tag="VNU03_25350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWP77873.1"
                     /translation="MTERASYYFDSYHERGSLRHVFHKRRIDSLAQLVPTPARVLDVG
                     CGSGVLCRMLADRGCTVTGVDLDPARVAWCRALVPEGEFSPGDVRSCRLATTYDAVIC
                     SEVLEHLGASDRQAALHNLVGHLRAGAILVVTVPSATYIRLEPVWERVRAWRYGPEGH
                     DDETCHQIVAPAQIEAGLRAAGCEVERAGSACCGLIRWWVARKR"
     gene            1717..>2581
                     /locus_tag="VNU03_25355"
     CDS             1717..>2581
                     /locus_tag="VNU03_25355"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWP77874.1"
                     /translation="MNADDLGLSEGVTDGIIQTMQRGIVTSTSLLANGLRTEAAARRL
                     GEIGCRSIGIHLNLTSGRPIGPIATRLLAPGGAFSGPARVWSLGVLGRLPGSAIEAEL
                     GRQIEAVKGLGVAVSHLDGHHHIHVLPQIAPIVAALARAHGIPAIRLPWAIGPGGLGL
                     TRCKWWLIRRCAARARGVFADAGLCMPDHFVAWYARGHRTRDELMTALDGLPPGVSEL
                     AVHPGRRDDTLAAVDRYVEQRTLELAALCDPAVRRHLHERGITLTSFADLCSEVAGRP
                     DVERDGRSSRRG"
BASE COUNT          373 a          865 c          915 g          428 t
ORIGIN      
        1 ccggcgacgt cggcgaatgg cattggctcg gctcctcggc ccgtgtggac ggtccctgcg
       61 gattgtacaa caggcccggc cggcaccttt gtaccggcag tttttacaat atcttgcgga
      121 gtcgtggagg gcccgctatg gaagggcttg ttaaatttca ggttcgcgcc cggcacggcg
      181 tatgcttacc agcggcaacc gccggggtgt cgccccgggg cgaaggaccg aaccggatcg
      241 ctcaaaggcg gcgaggaggg cgagcccgtg gggggtaggc cgggaagccg gatcaacgtg
      301 gatcgggagt ccggtgcgtc tctctatcgt cattcccgcg tataacagcg gcgggggtct
      361 cgcggacgtc gtcgcgagcg tcgtgggcca ttgtcgcgct cgcggcatgg ccttcgagct
      421 gctcgtcgtc gatgacggca gtgacgaaga aaacccgcgc ggccttggtc gccgcgcagc
      481 gtgggcaccg agagcttcga gtcctgcgca acgagcgcaa ccggggcaag ggatactcga
      541 tccgtcgggc ctacgccgag tgcgccggca cccacgtcgt gttcaccgac gccgatctgc
      601 cgtacgggct cgactccctc gaggctgccc tggccgcact ggagggcggc gccgatctcg
      661 tcatcggctc ccgggtactg cccggctcca tggtccggat gagctcacag cacttcccct
      721 acatcttcgc gcgccatctg ctgggccggg ccatgctgag agcggtcaat ctgatgttcg
      781 ggctcgacgt ctccgatacc cagtgtgggt tcaaggcgtg ccgattgtcg gtggcgcgct
      841 acctcgcgtc acgtctgagc atcgaccggt tcgcgttcga cgtggagctc atcctcgcgg
      901 cccggagggc cgggtaccac atcgccgaga tcccggtcga gctcaactat gccgggagaa
      961 tgacgacggt gcgggtgctc cgagacgcgc cctccgtcct gcgggatctt tgccagatca
     1021 aggtcgccga ctggcggggc cactatgagc cgagggaggc cgaccttcag tatcacgtcg
     1081 aatgacggag cgcgcctcct actacttcga ttcctaccac gagcgcggga gcctccggca
     1141 cgtctttcac aagcgacgga tcgattcgct cgcgcagctc gtccccaccc ccgcccgcgt
     1201 gctggacgtc ggctgtggct ccggggtact gtgccggatg ctcgccgatc gcggatgcac
     1261 cgtgaccggg gtggatctgg atccggcccg ggttgcatgg tgtcgagccc tcgtccccga
     1321 gggggaattc agcccgggtg acgtgcgctc gtgccggttg gccaccacct acgacgcggt
     1381 gatctgctcg gaggtgctgg agcacctggg ggcgagcgat cggcaggccg ccctgcacaa
     1441 cctggtcggc cacctccgag ccggggccat cctcgtggtc accgtgccgt cggcgaccta
     1501 tatccggctg gaaccggtct gggagcgcgt gcgggcctgg cgatacggac cggagggtca
     1561 cgacgacgag acgtgccacc agatcgtcgc tccggcccag atcgaggccg gcttgcgggc
     1621 ggcgggctgc gaagtcgagc gcgccggctc ggcctgctgc gggctgatcc gatggtgggt
     1681 ggcccgcaaa cgatgactcg cgcgcggagt ctgatcgtga acgcggacga cctcggtctc
     1741 agcgagggag tcaccgacgg gatcatccag acgatgcagc ggggcatcgt gaccagcacg
     1801 agccttctcg cgaacgggct gcggaccgaa gccgcggcgc gccggctcgg agagatcggc
     1861 tgccggtcga tcggcattca cctgaacctc acgagcggtc ggccgatcgg accgatcgcg
     1921 acccggctcc tcgcgcccgg tggggccttc tcggggccgg cgcgagtgtg gtcgctcggg
     1981 gtactcgggc ggcttccggg gtcggcaata gaagcggagc tgggaagaca gatcgaagcc
     2041 gtcaagggcc tcggggtcgc ggtgtctcat ctggatgggc accatcacat ccacgtgctc
     2101 ccccagatcg cgccgatcgt cgccgcgctc gcccgggccc acggcatccc ggcgattcgc
     2161 cttccatggg ccattggccc cggcggcctc ggtctcacgc ggtgcaagtg gtggctgatc
     2221 cgccgctgcg cggcgagggc caggggcgtc ttcgccgacg ccggcctgtg catgccggat
     2281 cacttcgtcg cctggtacgc gcgcggtcac cgcacgcgag acgagctcat gaccgcattg
     2341 gacggcctgc cccccggcgt ctcggagctg gccgttcacc ccggccggcg cgacgacacg
     2401 ctcgccgcag tggatcgcta cgtcgaacaa cgcaccctgg agctggccgc gctttgcgac
     2461 ccggccgtgc gacggcatct gcacgagcgg ggcatcaccc tgacgtcatt cgcggatctc
     2521 tgctcggagg tggccgggcg gccagacgtc gagcgcgatg gccggtcgag ccgccggggt
     2581 t
//
LOCUS       DATMCV010001109         6151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_796408, whole genome shotgun sequence.
ACCESSION   DATMCV010001109 DATMCV010000000
VERSION     DATMCV010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796408"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VNU03_25360"
     CDS             complement(<1..371)
                     /locus_tag="VNU03_25360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77875.1"
                     /translation="MADDISLSPLGRGQGEGANGEGPRRRTPRALRHVTFMIGLAITL
                     ALLATAAVSVVYAPRDPLEMSIAGRLQGPTAAHPLGTDQFGRDLLSRIMQGAITSILV
                     GVIAVGIGMVIGVAFGVLSGY"
     gene            complement(364..1308)
                     /locus_tag="VNU03_25365"
     CDS             complement(364..1308)
                     /locus_tag="VNU03_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77876.1"
                     /translation="MQRYIVRRVGALAVTLFFVSLLVFVVVRVLPGDPALIILGLEAN
                     ADSVARVRQALGLDQPVPVQYVQWVGHALRGDLGRSIQYDLPVGALILSRLNVTLPLT
                     LLAAGFMIAAAIPLGVFAATRHRRWGDYLTMTLSQLGVAVPGFWAGLLLILLFSVRLG
                     WVQSGGFDGWGEGFWRGLRSLLLPAIALGLFQFAVLARTTRSAVLEVLREEYVKTARA
                     KGVAEGVVLFRHALRNALIPIVTVAGVQLGQLLAGSIILESVFYLPGLGRLTLAAISA
                     RDLPVVQGVVLFVASMIVTVNAAVDILYGILDPRIRYG"
     gene            complement(1309..2811)
                     /locus_tag="VNU03_25370"
     CDS             complement(1309..2811)
                     /locus_tag="VNU03_25370"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77877.1"
                     /translation="MTTTIVIAALCAAAVAAPAVVSAQNPTSLVVQVPTEPPGLDMTS
                     NPSSAIAAVVFDNVQEGLIKIDRTGKMVPWLAERWYTSDNKNYTFFLRKGVRFSNGRE
                     MKAADVKFALDRAVNPETKHPYRVQYEAIQDVIQKDDYTVTVALKKVDATFLYTMARQ
                     GSVIYPREAVDALKSQPIGTGPFTVSDWVRGDRIVLLRNKDYWVKGLPKLDRVVFRFI
                     ADPNSTLAAIKSGDIDVSAFGIGPESVDELKKDARLSVIVGDTTNDVTLSMNNSKKPY
                     SDKRVRLAITHAINKDEVLKGAMFGYGKILGSNVDPTNPYFVDVSKRVAYDPAKAKKL
                     LAEAGYPNGFDAVFKVAPQYYYTVRSAEVVASQLAKVGIRATIQQIEWGQWLSQVFCL
                     APCQTPDYDMSIIGHAEAWDIGNFANPKYYFRWDSPEFQKIYQDSQVTVDDKSRRALY
                     VKMQEMLADEAPAVWLYMHPRLVITKKGVTGFWKDLPAPVQDLSEVAWQK"
     gene            complement(2817..3782)
                     /locus_tag="VNU03_25375"
     CDS             complement(2817..3782)
                     /locus_tag="VNU03_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWP77878.1"
                     /translation="MAPSSIFLVTGGAGFIGSHIVERLLAEGQRVRVFDNFSTGSRAN
                     LDFAKGNRRLEIVRGDLTNQKAVEKAVRGVTAVFHQAAMRSVPRSVADPLGANASNVT
                     GTLHVFHAAAQQKKKPRVVYASSSSVYGERPDLPKREDQPTAPISPYAATKVAGELYG
                     SVWHRLMGVETVGLRYFNVFGPRQDPNSEYAAVIPRFILWGQQGKPLQVHGDGTQSRD
                     FTYIDNVVSANLLAARGDAARVSGHAYNVGCGSRTSLLEIITMLEKMLGRSLTRRHSP
                     GRAGDVPHTLADVSAAKRDFGYEPLVDFDEGLRRTVEFFIGRKLG"
     gene            complement(3831..4379)
                     /locus_tag="VNU03_25380"
     CDS             complement(3831..4379)
                     /locus_tag="VNU03_25380"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWP77879.1"
                     /translation="MLPALETPRLRLRPLAAADLPDLIVLDLDPEVMRYVGSPAGVKT
                     PAETEARARARILEVERGVNEPLGFWRVEARADSAFLGVAALIRMPDGQDVEVAYRLA
                     RAAWGRGIATEAAGALVAHGLRTVGLPRLVAVTYPENRASQGVLDKLGFERRGLAEYK
                     GVQTTFHVLTREAWSACDRGRD"
     gene            4523..6061
                     /locus_tag="VNU03_25385"
     CDS             4523..6061
                     /locus_tag="VNU03_25385"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77880.1"
                     /translation="MRRVLVLGLAVLFAVGLGLVPAASAQTREETLIYALQSDIDNWD
                     PPNSVLREAIILGYHVFDHLAVRDLKTRKVMPNLATSWKAVDDTTWEVKLRQGVKFHD
                     GTPFTAKDVKATFDRVLDPTKKMTARGNHAKIKTVEIVDDYTVRFKTDGPYPLFVERL
                     TAQVMQSDKVIREKGHEWMQENPVGTGPYKLAKWSRKQEHLLVRNDDYWGPKPAFKYV
                     RVRIIPEQATQIAELLSGGVDIIKAVPPDQMDVIDKSGTARTATSPILRTAMLQLDQA
                     ARSGPNPFTDKRVRQAANMSTDIDGIIKHVLNGLGDRTATGINPMAFGWDAGLKPYKQ
                     DIAGAKKLLADAGFPNGVDVVFHEGPPVVEPAIQQTNDAIVADMTKAGFRVKRNYIGD
                     NTVLVARIKDGKAGSMFNWSWGYYSVFDADAILNDVFKCGENYSYYCNPALDDLISQG
                     RSTLDAKKRAEIYVKAQKLMFDDAAYLYKWGLRGVWGISNRIEYQAPADEVDRMFLVT
                     LRKK"
BASE COUNT         1031 a         2099 c         2109 g          912 t
ORIGIN      
        1 aaatagccgg agaggacccc gaacgccacc ccgatcacca tcccgatgcc gaccgcgatg
       61 accccgacca ggatcgaggt gatggcgccc tgcatgatcc gggagagcag gtcgcgcccg
      121 aactgatcgg tgccgagcgg gtgggccgcg gtcggaccct ggagccgccc cgcgatcgac
      181 atctcgaggg ggtcgcgtgg ggcgtagacc acgctgaccg ccgcggtggc caggagggcc
      241 agggtgatgg cgagcccgat catgaaggtc acgtgacgga gcgcgcgcgg cgtccggcgg
      301 cgcgggccct caccgttggc cccctcaccc tgccctctcc ccagagggga gagggagatg
      361 tcgtcagcca tagcggatcc ggggatcgag gatgccgtac aggatgtcga ccgccgcgtt
      421 cacggtcacg atcatcgagg ccacgaagag caccaccccc tgcaccaccg gcaggtcgcg
      481 ggcgctgatg gcggccaggg tgagccggcc gagccccggc agatagaaga ccgactccag
      541 gatgatggag ccggccagga gctggccgag ctgcacgccg gccaccgtca cgatggggat
      601 gagggcgttg cggagggcgt ggcggaagag caccaccccc tcggcgacgc ccttggcccg
      661 cgcggtcttc acgtactcct cccggagcac ctcgagcacc gcggaacggg tggtgcgcgc
      721 cagcaccgcg aactggaaga gccccagcgc gatcgcgggc aggagcagcg agcgcaggcc
      781 gcgccagaac ccctcgcccc agccgtcgaa cccgcccgat tgcacccagc cgagccgcac
      841 cgagaacagg aggatcagca gcagcccggc ccagaacccc gggaccgcga ccccgagctg
      901 ggagagggtc atggtcaggt agtcgcccca gcggcggtgg cgggtggccg cgaacactcc
      961 gagcgggatg gcggccgcga tcatgaaccc ggcggccagc agggtgagcg gcagggtcac
     1021 gttgaggcgc gagaggatga gggcccccac cggcaggtca tactggatcg agcgcccgag
     1081 atcaccccgg agggcgtggc cgacccactg cacgtactgg accgggacgg gctggtcgag
     1141 acccaaggcc tgccggaccc gcgccaccga atccgcgttg gcctcgagcc ccaggatgat
     1201 gagggcgggg tcgccgggca gcacccggac caccacgaag acgagcaggg agacgaagaa
     1261 gagggtgacc gcgagcgcgc cgacgcgccg gacgatgtac cgctgcaact acttctgcca
     1321 ggccacctcg gagagatcct gcaccggcgc gggcaggtcc ttccagaagc cggtcacgcc
     1381 cttcttggtg atgacgagtc gggggtgcat gtagagccag accgcgggcg cctcgtcggc
     1441 cagcatctcc tgcatcttga cgtagagcgc gcgccggctc ttgtcgtcca ccgtcacctg
     1501 cgagtcctgg tagatcttct ggaactcggg gctgtcccag cggaagtagt acttcgggtt
     1561 ggcgaagttg ccgatgtccc aggcctccgc gtggccgatg atcgacatgt cgtagtccgg
     1621 ggtctggcag ggcgccaggc agaagacctg ggagagccac tggccccact cgatctgctg
     1681 gatggtggcg cggatgccga ccttggcgag ctggctcgcc accacctcgg cggagcgcac
     1741 cgtgtagtag tactggggcg ccaccttgaa gaccgcgtcg aagccgttcg gataccccgc
     1801 ctcggccagg agcttcttgg ccttcgcggg atcgtaggcg acccgcttgg agacgtccac
     1861 gaagtagggg ttggtcggat ccacgttgga gccgaggatc ttcccgtagc cgaacatcgc
     1921 ccccttcagg acctcgtcct tgttgatcgc gtgggtgatg gccaggcgca cccgcttgtc
     1981 cgagtacggc ttcttgctgt tgttcatcga gagcgtcacg tcgttggtgg tgtcgcccac
     2041 gatgaccgag aggcgcgcgt ccttcttcag ctcgtccacg ctctcgggcc cgatgccgaa
     2101 ggcggagacg tcgatgtcgc cggacttgat cgcggccagg gtggagttgg gatctgcgat
     2161 gaaccggaac accacccggt cgagcttggg cagccccttc acccagtagt ccttgttccg
     2221 gagcaggacg atgcggtcgc cccgcaccca gtccgacacc gtgaacggcc cggtgccgat
     2281 cggctggctc ttgagcgcgt ccaccgcctc gcggggatag atcaccgagc cctggcgcgc
     2341 catggtgtag aggaaggtgg cgtcgacctt cttgagcgcc accgtcaccg tgtagtcgtc
     2401 cttctggatg acgtcctgga tcgcctcgta ctgcacgcgg taggggtgct tggtctcggg
     2461 gttcaccgcc cggtccagcg cgaacttgac gtcggccgcc ttcatctcgc ggccgttcga
     2521 gaagcggacg cctttgcgca agaagaacgt gtaattcttg ttgtcgctcg tgtaccaccg
     2581 ctcggccagc cacggcacca tcttgccggt gcggtcgatc ttgatcaggc cttcctgcac
     2641 gttgtcgaag acgaccgccg cgatggcgga ggacgggttc gacgtcatgt cgaggccggg
     2701 cggctcggtt ggcacctgga ccaccaggct ggtcgggttc tgggcggaga ccacggcggg
     2761 ggcggcaacc gccgccgcgc agagcgcggc gatcacgatg gtagtggtca cacgcatcat
     2821 ccgagtttcc tcccgatgaa gaattcgacc gtgcgccgca gaccttcgtc gaagtccacc
     2881 agtggctcgt acccgaagtc gcgcttggcc gcgctcacgt cggccagggt gtgcgggacg
     2941 tcccccgccc ggccgggcga gtgccggcgg gtgagggacc ggccgagcat cttctcgagc
     3001 atcgtgatga tctcgagcag gctcgtgcga ctgccgcagc ccacgttgta agcgtgcccg
     3061 gagacgcggg ccgcgtcgcc gcgggccgcc agcaggttgg ccgagaccac gttgtcgatg
     3121 taggtgaagt cgcgcgactg ggtgccgtcg ccgtgcacct gcagcggctt gccctgctgg
     3181 ccccacagga tgaagcgcgg gatcaccgcc gcgtactcgc tgttggggtc ctggcgcggc
     3241 ccgaacacgt tgaagtagcg caggccgacc gtctccacgc ccatgaggcg gtgccagacc
     3301 gagccgtaga gctcgccggc caccttggtg gccgcgtagg gcgagatcgg cgcggtgggc
     3361 tggtcctccc gcttgggcag gtcggggcgc tccccgtaga ccgaggagga cgacgcgtag
     3421 accacccgcg gcttcttctt ctgctgggcc gccgcgtgga agacgtgcag ggtcccggtc
     3481 acgttggagg cgttggcccc cagcggatcg gccaccgacc ggggcaccga gcgcatcgcg
     3541 gcctggtgga acaccgcggt caccccgcgc accgccttct cgaccgcctt ctggttggtc
     3601 aggtccccgc ggacgatctc gaggcgccgg ttccccttgg cgaagtcgag gttggcccgg
     3661 ctcccggtgg agaagttgtc gaatacccgg acgcgctggc cctccgcgag gaggcgctcg
     3721 acgatgtgcg agccgatgaa gcccgcgccg ccggtgacca ggaatatcga tgagggagcc
     3781 atgagtgtgc gtgaagaacg gtcaattatg gcggggcggc cggaacaaag tcaatctcgg
     3841 ccacggtcgc acgcgctcca cgcctcgcgg gtcagcacgt ggaaggtggt ctgcacgccc
     3901 ttgtactcgg cgagcccccg gcgctcgaag cccagcttgt cgagaacgcc ctgggaggcc
     3961 cgattctccg ggtaggtcac cgcgaccagc cgcgggaggc ccacggtgcg cagaccgtgc
     4021 gcgaccagcg cgcccgccgc ctcggtggcg atcccccgcc cccaggcggc ccgggccagg
     4081 cggtaggcga cctcgacgtc ctgcccgtcc ggcatccgga tcagcgcggc cacgccgagg
     4141 aaggccgagt cggcgcgtgc ctccacgcgc cagaagccga gcggctcgtt cacgccacgc
     4201 tcgacttcca ggatgcgcgc gcgcgcccgc gcctccgtct ccgccggcgt cttcaccccc
     4261 gcggggctgc ccacgtagcg catcacctcg gggtcgaggt cgagcacgat caggtcgggc
     4321 aggtcggcgg cggccagcgg gcgcaggcga agacgcggcg tctcgagcgc ggggagcatg
     4381 gcgtgcccca tctcttagca cacccgggtc cgcggccgcg ggacgccttg actgcccggg
     4441 ccctcgcggc tattgttcct gcacccagat gccccgttcc cttcgtgcgc cagccgtcac
     4501 gattcctccc aaggaggtcc tcatgcgtcg tgtactcgtc ctcgggctcg ccgtcctgtt
     4561 cgccgtcggt ctcgggctcg tcccggcagc ctcggcccag acgagggagg agaccctcat
     4621 ctacgcgctg cagtcggaca tcgacaactg ggatccgccc aactcggtgc tgcgcgaggc
     4681 gatcatcctc ggctaccacg tgttcgatca cctggcggtg cgtgatctga agacccgcaa
     4741 ggtgatgccg aacctggcca cgtcctggaa ggcggtcgac gacaccacct gggaagtgaa
     4801 gctccggcag ggcgtcaagt ttcacgacgg caccccgttc accgccaagg acgtgaaggc
     4861 caccttcgac cgcgtcctcg atccgaccaa gaagatgacc gcgcggggca accacgccaa
     4921 gatcaagacg gtcgagatcg tggacgacta caccgtccgc ttcaagaccg acgggccgta
     4981 tccgctgttc gtggaacgac tgaccgccca ggtcatgcag tccgacaagg taatccgcga
     5041 gaagggccac gagtggatgc aggagaaccc ggtgggcacc gggccgtaca agctcgcgaa
     5101 gtggagccgc aagcaggagc acctcctggt ccgcaacgac gactactggg gcccgaagcc
     5161 cgcgttcaag tacgtccggg tccggatcat ccccgagcag gcgacccaga tcgcggagct
     5221 gctctcgggc ggcgtcgaca tcatcaaggc ggtgccgccc gaccagatgg acgtgatcga
     5281 caagtcgggg acggcgcgca cggccacctc tcccattctg cgcaccgcga tgctgcagct
     5341 cgaccaggcg gcccgcagcg gccccaatcc gttcaccgac aagcgggtcc ggcaggccgc
     5401 caacatgtcg acggacatcg acgggatcat caagcacgtg ctgaacgggc tcggtgaccg
     5461 caccgccacc ggcatcaacc cgatggcgtt cggctgggac gcgggcctca agccctacaa
     5521 gcaggacatc gcgggggcca agaagctgct cgcggacgcc ggcttcccca acggggtcga
     5581 cgtcgtcttc cacgagggcc cgccggtggt ggagcccgcg atccagcaga ccaacgacgc
     5641 gatcgtggcc gacatgacca aggccggctt ccgggtgaag cgcaactaca tcggcgacaa
     5701 cacggtgctg gtggcccgca tcaaggacgg caaggccggc tcgatgttca actggtcctg
     5761 gggctactac tcggtcttcg acgcggacgc gatcctgaac gacgtgttca agtgcggcga
     5821 gaactacagc tactactgca accccgcgct cgacgatctg atcagccagg gtcgctccac
     5881 cctcgacgcc aagaagcgcg ccgagatcta cgtcaaggcc cagaagctca tgttcgacga
     5941 cgcggcctac ctctacaagt ggggcctccg cggggtgtgg ggcatctcga accgcatcga
     6001 gtaccaggcc cccgccgacg aggtcgaccg gatgttcctg gtgaccctga ggaaaaagta
     6061 ggcgccgccg ccccgggccg gtcgcattcg cacgatgaag cgctacgcct tccgccaggt
     6121 ggtccagctc gccgtcgtca tcgtcgggat c
//
LOCUS       DATMCV010001110         4600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_729068, whole genome shotgun sequence.
ACCESSION   DATMCV010001110 DATMCV010000000
VERSION     DATMCV010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729068"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..313)
                     /locus_tag="VNU03_25390"
     CDS             complement(170..313)
                     /locus_tag="VNU03_25390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77881.1"
                     /translation="MGDPRNTTNRLSQGSPYAYRVDAVFEGARGAGNRADDVRPCSLT
                     FVK"
     gene            complement(368..1261)
                     /locus_tag="VNU03_25395"
     CDS             complement(368..1261)
                     /locus_tag="VNU03_25395"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010141997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP77882.1"
                     /translation="MERTAVLGFVGLGVMGEAMCRNLARKSGATVVAFDERPAPLAAL
                     AAGGVQAAASVAEVARRAEIVFLCLPGEPQVRAVCLGAGGGLAALVEPGQTVVDMSTC
                     PVALARELHQAFGVRGAAFADAPIARTAQAARDGTLSIMVGGEPALFARLRPLLACMG
                     SEVTHCGGVGAGQAVKLMNNMVVAQTVVALAEALAVARASGAVEPRVLFETLAKGSAD
                     SFVLRNHGMQSLLLDHHPTEGAFPTRYILKDLSYAIQLAESAGVALEQAATTRRLLEA
                     TAAAGWSEAYYTAVIRIIEKR"
     gene            1511..3118
                     /locus_tag="VNU03_25400"
     CDS             1511..3118
                     /locus_tag="VNU03_25400"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP77883.1"
                     /translation="MDKSGWVMGEVLARQARARGDRPFLTLQNGPDVSYAEADEIAHR
                     VSRGVAALGAGPGDRVGVWLPNGLEILHASFGIYRAGAVAVFVNPGYRGIFLEHVLNN
                     AGARWVIMAAEYLPRLAESEANLPGVERAVIVGPVPPDAPRLRRIVIQSWDELVAQPA
                     DSLGVPVSYRDVGAMMYTSGTTGPSKGVLMPHAHLHLFGQGVVDNLRVTADDVYYICM
                     PLFHANALCMQLYGTLIAGARAVIAPQFSASRWLDEVRACGATVTNTLGVMTEFIHRQ
                     PARPDDRRHRLRLICAVPTPAEIGPSFRERFGVPLIEGYGMTEVNIPLYMPLDVPYRP
                     GSCGKVYDRYFEVRVVDPETDEEMPRDSVGEIVVRPREPFGFMLGYNAMPDRTVEAWR
                     NFWFHTGDAGRQDPDGYFYFVDRIKDTIRRRGENLSSYEIERVLIEHPAVAEAAAVAV
                     RSEIAGGEDEVKACVVLTLGQALSPEALLDFCQTRMPHFAVPRYVEFLAELPKTPTAK
                     VQKVALRRSGITAATWDREAAGYRVRR"
     gene            3476..>4600
                     /locus_tag="VNU03_25405"
     CDS             3476..>4600
                     /locus_tag="VNU03_25405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77884.1"
                     /translation="MRILIAEDDTTSRIILEEILTRLGHEVVAVTNGQEAWATFQQDH
                     VSLVISDWMMPDLNGLELCRRIRAESRPKYTYIILLTALEGKASYLEAMEAGADDFAT
                     KPLDSDQLHARLRVAERILGLQRDLVQLELSQQQIVQQERLRALGEMASGIAHDFSNA
                     LSSIVGFSSLLIQHPAELDDRKKVLQRLELIHTAGLGAAQLVRRLREFCRHRDETEAF
                     LTVDVNEVVRTAVALTEPRWKAQARAAGVSIHVETDLATVPAVDGNVSELRDVLMNLI
                     FNAVDAMPQGGTLRIATTADAGRTPVLVTVTDTGAGMTDEVRRRCLEPFFTTKGERGT
                     GLGLAMAYGVIQRHRGTFDIASQPGQGTTFRIRLPARSDRS"
BASE COUNT          695 a         1584 c         1630 g          691 t
ORIGIN      
        1 ccggtcgagg ggccgtttcg acggccaatc ggggtgatcc accggcggac gcgcgagctg
       61 tcggcggcgg gccgggcctt cctgacctcg ctcaccaccg agctcatgcc acccaaccca
      121 ggcgcccgta gccacgtccg gcgcggagcc agaccgaccc ggccgtcttc tacttgacga
      181 aggtcaggct acacgggcgg acgtcgtcgg cacgattgcc ggccccgcgc gcgccctcga
      241 agacggcgtc gacccgatac gcgtacgggg agccctggct caaccggttg gtggtgttcc
      301 tcggatcgcc caccagtcca cgggcgtcga tgctggcctt gccgtccggc tggatcgtcc
      361 cgctgggcta tcgtttctcg atgatgcgga tcaccgccgt gtagtaggcc tcgctccatc
      421 ccgcggcggc ggtcgcctcg agcagccgcc gtgtggtggc cgcctgctcg agcgcgacgc
      481 ccgcggactc cgccagctgg atggcgtaag agaggtcctt caggatgtac cgggtgggga
      541 aggcgccctc cgtggggtga tggtcgagca ggagcgactg catcccgtga ttgcggagga
      601 cgaagctgtc ggcggacccc ttggcgagcg tctcgaacaa cacgcgcggc tccactgcgc
      661 cggaggcgcg ggccaccgcg agggcctcgg cgagggcgac cacggtctgg gcgaccacca
      721 tgttgttcat gagcttcacc gcctggcccg cgccgacgcc tccgcagtgg gtcacctccg
      781 aacccatgca cgccaggagc ggccggagcc gggcgaagag cgccggctcc ccacctacca
      841 tgatgctgag cgtgccgtcg cgggcggcct gcgcggttcg cgcgatgggc gcgtcggcga
      901 aggcggcgcc gcgcacaccg aaggcctggt ggagctcgcg cgcgagcgct accggacagg
      961 tgctcatgtc gaccaccgtc tggcccggct ccacgagggc ggcgaggcct ccgccggcgc
     1021 cgaggcagac cgcgcgcacc tggggttctc ccggcaggca gaggaagacg atctccgcgc
     1081 ggcgcgccac ctcggccacc gaggccgcgg cctgcacgcc gccggcggcg agcgcggcga
     1141 gcggcgccgg ccgctcgtcg aaggccacca ccgtggcccc cgactttctc gccaggtttc
     1201 gacacatcgc ctcgcccatg accccgagcc cgacgaatcc gagcacggcc gtcctctcca
     1261 tgcctgcctc cctgtgcccc gagtgttcct gcccgacgcg cggctctcgt cgaacggcgc
     1321 ggccggcgga agacggcccg cgaagagggg cgcgcgccac ctgatagtat cgcgggtcga
     1381 ctccgatggg cggtaccgcc gatcaccttg atcgcgcagg cgaactgctc gggcggcgag
     1441 gtggcggctt gatgtagagt gccagggcgg cggcgggagc gagtgcattc cagcccagcg
     1501 ggaggacggc atggacaagt cgggctgggt gatgggggag gtgctggcca ggcaggctcg
     1561 cgcacgaggt gatcggcctt ttctcacgct ccagaatggg cccgacgtca gctacgccga
     1621 ggccgatgag atcgcccacc gggtgagccg cggggtggcc gccctggggg cggggcccgg
     1681 ggaccgggtg ggcgtgtggc tccccaacgg gctggagatc ctgcacgcct cgttcggcat
     1741 ctaccgggca ggggccgtcg cggtgttcgt caatccgggc taccggggca tcttcctcga
     1801 gcacgtgctg aacaacgcgg gagcacgctg ggtgatcatg gcggccgaat atctgccgcg
     1861 tcttgccgag agcgaggcca acctacccgg cgtcgagcgg gccgtgatcg tgggacccgt
     1921 gccgcccgat gcccctcggc tccgccggat cgtgatccag tcctgggacg agctggtggc
     1981 ccagccggcc gactcgctcg gcgttcccgt gagctatcgg gacgtgggcg ccatgatgta
     2041 cacgtccggg accaccgggc cctccaaggg cgtgctgatg ccccacgccc acctgcacct
     2101 cttcggccag ggcgtggtgg acaacctccg ggtgacggcc gacgacgtct actacatctg
     2161 catgccgctg ttccatgcca acgcgctgtg catgcagctc tacggcacgc tcatcgcagg
     2221 ggcccgggcg gtgatcgccc cccagttcag cgccagccgc tggctggacg aggtgcgcgc
     2281 gtgcggggct accgtcacca acaccctggg ggtgatgacg gagttcatcc atcgccagcc
     2341 ggcgcgtccg gacgaccggc gccatcggct ccgcctgatc tgtgccgtgc caacgccggc
     2401 ggaaatcggc ccgagctttc gcgagcgctt cggggtgccg ctcatcgagg ggtacggcat
     2461 gacggaagtg aacatccccc tctacatgcc gctcgacgtg ccgtaccgcc cgggaagctg
     2521 cggaaaggtc tacgaccgct acttcgaggt ccgggtggtg gaccccgaga cggacgagga
     2581 gatgccgagg gacagcgtgg gcgagatcgt cgtgaggccc cgggagccgt tcggcttcat
     2641 gctcgggtac aacgccatgc ccgaccggac ggtggaagcg tggcgcaact tctggttcca
     2701 caccggcgac gccgggcgcc aggacccgga cggctacttc tacttcgtgg accgcatcaa
     2761 ggacaccatc cggcgccgcg gcgagaatct ctcctcctac gagatcgagc gcgtgctgat
     2821 cgagcatccc gcggtggccg aggccgccgc cgtcgcggtc cgctcggaga tcgcgggagg
     2881 cgaggacgag gtcaaggcgt gtgtcgtgct cacgctcggg caggcgctct cccccgaggc
     2941 gctgctggac ttctgccaga cgcgcatgcc ccacttcgcg gtgccccgct acgtggagtt
     3001 cctggccgag ctcccgaaga cgcccacggc caaggtgcag aaggtggcgc tgcgccggag
     3061 cgggatcacc gccgccacct gggatcgcga ggcggccggc taccgcgtgc ggcgctgatt
     3121 cgtcgcgtcc ggacggccgc caactgcttg gcggcggcga gtgaggcgtg gccgccacat
     3181 tggcctgctc gtgtcccgtc tgtgggttgg gtgtgccgat tcccgtcagg ccgcccgaca
     3241 ggatgtgaca atccccagtg gcccggggga tgcccggact ccatcgagga agagatctgc
     3301 tccatcatcc cggattcgtg agcactgtgc ggcagtcgcg tccggccgat tgggcgcgcc
     3361 agaccgcgcc ttggtggcac tcgagttgca acgatctcga cgatggtccg ggggttgttc
     3421 gggtcaccct gaagcatccc gcgtagcctc agacgacgag ccctcgggcg cacccatgag
     3481 aatcctgatc gcagaggatg acacgacgtc gaggatcatt ctcgaagaga tcctgacccg
     3541 cctcggtcat gaggtcgtgg cggtgacgaa cggtcaggag gcctgggcga cgtttcagca
     3601 ggatcacgtg tccctcgtca tctccgactg gatgatgccg gatctaaacg gcctcgagct
     3661 gtgtcgtcga atccgcgccg agagccgccc gaagtacacg tacatcatcc tgctgaccgc
     3721 gctggaaggg aaggcgagct acctcgaggc gatggaagcg ggcgcggacg acttcgcgac
     3781 caagccgctc gattcggatc agcttcatgc ccggctccgt gtcgcggagc ggatcctcgg
     3841 tctgcaacgg gacctggtgc agctcgagct gagccagcag cagatcgtgc agcaggagcg
     3901 gctccgggca ctcggggaga tggccagtgg catcgcgcac gacttcagca acgcgctgtc
     3961 gtcgatcgtc ggcttcagta gcttgctgat ccagcatccc gccgagctgg acgatcgcaa
     4021 gaaggtattg cagcggctgg agctgatcca caccgcgggt ctcggggccg cccagctcgt
     4081 ccgccgcctc cgcgagttct gccgccaccg ggacgaaacc gaggccttcc tgaccgtcga
     4141 cgtgaacgag gtcgtccgca ccgcggttgc gctcaccgag ccgcgctgga aggcgcaggc
     4201 gcgggcggcc ggcgtctcca tccacgtgga gaccgacctc gcgaccgtgc ccgcggtgga
     4261 cgggaacgtc tccgagctcc gggatgtgct gatgaacctg atcttcaacg cggtggacgc
     4321 catgccgcag ggcggcaccc tgcgcatcgc gaccaccgcg gacgccggcc gcacgccggt
     4381 gctcgtgacg gtcacggaca ccggcgccgg aatgaccgac gaggtgcgtc ggcggtgcct
     4441 ggagccgttc ttcacgacca agggcgagcg gggaacgggc ctgggactgg cgatggcgta
     4501 cggggtcatc cagcgtcacc gcggcacatt cgacatcgcg agccagcccg gccagggcac
     4561 gacgttccgc atccggttgc ccgcgcgctc ggatcgatcg
//
LOCUS       DATMCV010001111         2587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_796650, whole genome shotgun sequence.
ACCESSION   DATMCV010001111 DATMCV010000000
VERSION     DATMCV010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796650"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VNU03_25410"
     CDS             <1..230
                     /locus_tag="VNU03_25410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77885.1"
                     /translation="DGLHDYLDRAFLGVRGVKVIMDRRERERRGSERRASDDRRQVER
                     RQPRGISYALGYTTVRFGASSEDVRAEAAIG"
     gene            complement(247..579)
                     /locus_tag="VNU03_25415"
     CDS             complement(247..579)
                     /locus_tag="VNU03_25415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77886.1"
                     /translation="MTEDPAADLVRWVKEGEHLFRLTLESLQGAAALGSKVEELSQEA
                     RRLRDENDTLRRELDLLRAERLEVADTFKAFAEHVTRLATLALHRLGNPGLLTDEAAR
                     APQTPRRP"
     gene            629..1249
                     /locus_tag="VNU03_25420"
     CDS             629..1249
                     /locus_tag="VNU03_25420"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWP77887.1"
                     /translation="MRLTIGLQILGALVASLLLVGAFTPAVNLLAYWRAPARVLGPVD
                     AIVVLGEGGITESGRLSHSSLQRITEAIDLYRQGLAPILVVSGSPSRVARPEAEARVQ
                     IARASGIAPEAVLALNPVRTTREEAQAVRALLEPRGARRIILVTDGASMARSVGTFEK
                     VGFTVTPSYGVPILRWGGGPGARLRLLQENLIEMLASAYYRIQGWT"
     gene            1258..1581
                     /locus_tag="VNU03_25425"
     CDS             1258..1581
                     /locus_tag="VNU03_25425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77888.1"
                     /translation="MVLEQLFLLALVAVTSVAAYALGIAGLGLRPARLWEAGRFALQL
                     VGMSTLFFVGNLAIGLVCVLAVRGATGTFVSVYLLNDPTLGVLSAAQGVCFECWRTSG
                     RRDSR"
     gene            1578..>2587
                     /locus_tag="VNU03_25430"
     CDS             1578..>2587
                     /locus_tag="VNU03_25430"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWP77889.1"
                     /translation="MTERPGRAALRNAGLLLVQRTGLVIAGVGFAAVVPRLLGPDVYG
                     RYALVAALAVVFAACSGLGVNEIIGRHGAVLAAERDQAGLDRLFGSLLALRVATGVLA
                     GVAFGVLTFFWLPDLDRVGLAAAAFAVMARGIWLALFSLFLGLNQAARWGAGELIDRL
                     AVLALLPLGAMASGFRGACVGLLASQLIVLAAGLWWARAFVSLRRPDRRYLSPYLRFG
                     LAFFGSNLLGIAFQGSGEALVRALTGDYAQVSYFGVANGAFLTGVAAIQQLSLAFVSA
                     MVILRQEGKGTMVDDAARRLVTGLTAGSMVVALTALLVAPDLIPAVVGRDYGPVAANL
                     VL"
BASE COUNT          291 a          892 c          953 g          451 t
ORIGIN      
        1 aggacggcct gcacgactac ctggatcggg ccttcctggg cgtgcgcggg gtgaaggtga
       61 tcatggaccg gcgcgaacgg gagcgccggg gctccgagcg tcgggcgagt gacgaccggc
      121 gtcaggtgga gcgccggcag ccgcggggca tctcctatgc cctcggctac accacggtgc
      181 gcttcggcgc gtcctccgag gacgtgaggg cggaggcggc gatcgggtga gcttggccgg
      241 ggcgccctag ggccggcgcg gggtctgggg ggcgcgcgcc gcctcgtcgg tgagcaggcc
      301 agggttgccc aggcggtgca gggcgagcgt cgccaggcgg gtgacgtgct cggcaaaggc
      361 cttgaaggta tccgcgacct cgagccgctc tgcccggagg aggtcgagct cgcgcctgag
      421 ggtatcgttc tcatcccgca gccggcgtgc ctcctgggac agctcctcga ccttcgatcc
      481 gagcgcggcc gcgccctgca ggctctccag ggtgagccgg aacaggtgct cgccctcctt
      541 cacccagcgt acgaggtccg cggctggatc ttcagtcatg ggcgggtatc ttaccgcaag
      601 cgaccgtgcg atgatgaccc cgcgcgcaat gcgactcacc atcggtctcc aaatcctcgg
      661 ggccctggtg gcgagtctcc tcctggtggg cgccttcacc cccgcggtca accttctggc
      721 ctactggcgc gccccggcgc gggtgctcgg tcccgtcgat gccatcgtgg tgctgggcga
      781 gggtgggatc accgagagcg gccgactcag tcattcctcc ctgcagcgga tcaccgaggc
      841 catcgatctc taccgccagg ggctggcccc gattctcgtg gtctccggct ccccgtcccg
      901 ggtggcccgc cccgaggcgg aggcgcgggt ccagatcgcc cgcgcgagcg gcatcgcgcc
      961 ggaggcggtc ctggcgctca accccgtccg caccacccgc gaggaagccc aggcggtccg
     1021 ggcgctgctc gagccacgcg gcgcccggcg catcatcctg gtgacggacg gagcgtcgat
     1081 ggcccgctcg gtggggacct tcgagaaggt cgggttcacg gtcaccccgt cgtacggggt
     1141 gccgatcctg cgctgggggg gcggtcccgg cgcgcgtctg cgcctcttgc aggagaacct
     1201 gatcgagatg ctggcctccg cctactaccg catccagggc tggacctgag ccgcgccgtg
     1261 gtcctcgagc agctgttcct gctggctctc gtcgccgtga cgtcggtcgc ggcctacgcc
     1321 ctcgggatcg ccggcctcgg cctgcggccg gcccggctat gggaggcggg acggttcgcg
     1381 ctgcagctgg tcgggatgag caccctgttc ttcgtgggca acctcgccat cgggctggtc
     1441 tgcgtgctgg cggtccgcgg ggcgaccggc acgttcgtct ccgtctacct gctcaacgac
     1501 ccgaccctgg gcgtgctctc cgcggcccag ggcgtctgct tcgagtgctg gcggacgagc
     1561 ggaaggcgtg actcccgatg accgagcgcc ccggtcgggc ggctttgcgg aacgcgggtc
     1621 tcctgctcgt ccagcggacg ggcctcgtca tcgcgggcgt gggcttcgcg gcggtggttc
     1681 cgcgcctgct cgggccggac gtgtacggcc gctacgcgct ggtggccgca ctcgccgtcg
     1741 tgttcgccgc ctgtagcggg ctcggggtca acgagatcat cgggcgccat ggtgcggtcc
     1801 tcgccgcgga gcgagaccaa gccggattgg atcgactctt cggtagcctg ctcgcactgc
     1861 gcgtggccac gggcgtgctc gcgggagttg cctttggcgt gttgaccttc ttctggctgc
     1921 ccgacctgga tcgggtgggc ctggctgcgg cggccttcgc ggtgatggcg cgggggatct
     1981 ggctggccct cttctcgctg tttctgggcc tcaaccaggc ggcccggtgg ggcgccggcg
     2041 agttgatcga ccgcctggcg gtgctggccc tgttgccgct cggcgcgatg gccagcggct
     2101 tccgcggcgc ctgtgtcggg ctcctggcca gccagctcat cgtgctcgcg gccggcctgt
     2161 ggtgggcccg cgccttcgtc tcgctgcgcc gtccggaccg caggtacctt tcaccctacc
     2221 tgcgcttcgg cctggccttc ttcggctcga acctgctggg catcgccttt caagggagcg
     2281 gtgaggcact ggtgcgcgcc ctgaccggcg actacgcaca ggtgagctat ttcggcgtcg
     2341 ccaacggggc attcctgacc ggcgtcgccg ccatccagca gctgagcctg gcgttcgtca
     2401 gcgccatggt catccttcgt caggaaggca aagggacaat ggtggacgac gcagctcggc
     2461 ggctggtgac cgggctcacc gcgggcagca tggtggtcgc gctcaccgca ttgctcgtgg
     2521 cgccagacct catcccggcg gtggtcgggc gcgactatgg gccggtggcc gcgaaccttg
     2581 tcttgat
//
LOCUS       DATMCV010001112         5038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_59911, whole genome shotgun sequence.
ACCESSION   DATMCV010001112 DATMCV010000000
VERSION     DATMCV010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59911"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VNU03_25435"
     CDS             <1..546
                     /locus_tag="VNU03_25435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77890.1"
                     /translation="EATRQIVDEVAASVSAAASAAPPAAPAEEPARQPRNVRVLLVPV
                     WDEADLLGLSMLQHLVSPAHWTIDLTSPHLLAAEVVALVEETRPAVVCLGAIPAGSRS
                     THTRYLCRRLRARFPDLRIIVGRWGLRAAEARARRQLEAAGADSVGVTLVETREHLQR
                     VHSLEPPTLRPPAEAEPALRS"
     gene            complement(536..2440)
                     /locus_tag="VNU03_25440"
     CDS             complement(536..2440)
                     /locus_tag="VNU03_25440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWP77891.1"
                     /translation="MPSDPGFGEKLRTVFGLSPTILCLTGVSDGRIRDANDAFLATTG
                     YRREEILGRTPVELGLWVDPSQRDDGIRELKAGRPVRSIECRFRVKNGDIRVTVLSAD
                     IVEFDGDACILSALTDITDRTRADEALRESERRFMVAFHANPLPMSITSLRDHRHLEV
                     NEAATRHGGYSREEMLGKSKMDLGFWVTGEQRERMLGLLHQEGRVRDFEVAFLTRAGE
                     ERRLLVNSEVITFGGEPAVLSVSLDITERSQHEAHVRERREEAEALARSLREETQAKD
                     EFLAMLGHELRNPLGTLSNAVAVLERLQGDETMRHVVAIIGRQTGHLARLVDDLLDVA
                     RVTSGKIDLQRRAVELHALARRCLDALAQAGRTERHDVRLEGAAVHVHADPARLEQVV
                     NNLLDNALKYTPPGGRVQVTTEAVGDTGVLRVRDSGQGIRTDLLGRVFDLFVQEPQSL
                     DRSRGGLGLGLALVKRLVELQGGAVAAWSAGPGQGSEFTVRLPAIAAPADEAAGSEEA
                     TRATVVPRRVLVVEDSPDARQSLRLLLEMAGHHVETSEDGPSGLAKLGAFRPDVALID
                     LGLPGMDGYAVARELRRRPDTRAIRLVAVTGYGQADDRRRALAAGFDQHVTKPVDATM
                     LDELLGRPSS"
     gene            2548..3423
                     /locus_tag="VNU03_25445"
     CDS             2548..3423
                     /locus_tag="VNU03_25445"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWP77892.1"
                     /translation="MTSQASAGYEIDVQDVEYLRHGDWPLLARLYSPRGDGPFPMIVD
                     LHGGAWCRGDRTNDAVMNEPLARSGVVVAALDFRMPPDASYPGSLVDISYGIRWLKSR
                     ATTLRGRPDLVGIMGASSGAHQAMLLAMKPRDPKYAGLPLPAGVSAVDGSVRCAVLCW
                     PVIDPLGRYHYAKKLKAGGAPYPEVVDRVLPGHDQYWITEDAMAEGNPTLALERGESV
                     ELPPVLYIQGDRDMAHPRPDLDRFVAGYRKAGGALDLELLAGEAEGYISRKPASPAVD
                     RTIARIIEFVHKQLA"
     gene            complement(3420..4475)
                     /locus_tag="VNU03_25450"
     CDS             complement(3420..4475)
                     /locus_tag="VNU03_25450"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP77893.1"
                     /translation="MPPLPRFGLNRFDSRSVDAFAADVRRAEQLGWDAALQPDSQLRR
                     RDTYVLLAAAARVTERIVLGPLLANPVNRHPTVTASSIATVDELAPGRVLLGWGVGDT
                     AVRLAGLKPARVNELEVSTRLMRALLDGQAVEVGAVRPARLPHHRPVPIWIAAGGPRT
                     LRMAGGVADGVFIRVGTHPANIATAVNAIRAGAVAAGRDPASVRLGAVFHTVLVDDAE
                     RALVMARSMAAGYYEYSPALFDPPGLAWSGPDPESLKHARGVWPDFHHATDLEASGRA
                     VDFLPGSAADAFSLRGAPPEVARQLVTALRDAPAPFDYVVLHPIPDPKWPGDPERDYT
                     ARVAREVLPLVRRALGG"
     gene            complement(4511..>5038)
                     /locus_tag="VNU03_25455"
     CDS             complement(4511..>5038)
                     /locus_tag="VNU03_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing (seleno)protein"
                     /protein_id="HWP77894.1"
                     /translation="HRLAELYGIVNVPQAVWIDEAGRIVRPAEPAGAYEGFRKMNRAT
                     REMPEDVARLTAQAKATYLDAIRDWVRLGPKSSHALTAAQAREHLPTPREDTARAQAM
                     FRLGQLLIRRGEHEEGERWIHEASRLHPDSWCIWRQGAAVNETGLAALPEFWQRVDAL
                     GDARYYARVDMPGMP"
BASE COUNT          642 a         1899 c         1834 g          663 t
ORIGIN      
        1 gaggccacgc gccagatcgt cgacgaggtc gccgcgagcg tgagcgccgc cgcgagcgcc
       61 gccccgcccg cggccccggc cgaggagccg gcgcgccagc cgcgcaacgt ccgcgtgctc
      121 ctggtgccgg tgtgggacga ggccgacctc ctcggcctct cgatgctcca gcacctcgtg
      181 agcccggccc actggacgat cgacctgacc tcgccccatc tcctcgccgc cgaggtcgtc
      241 gccctcgtcg aggagacccg gcccgcggtg gtctgcctgg gcgcgatccc cgcgggtagc
      301 cgcagcacgc acacccgcta tctctgcagg cggctgcgag cgcgcttccc ggacctccgg
      361 atcatcgtgg gacgctgggg cctgcgggcg gccgaggccc gcgcgcggcg gcagctcgag
      421 gccgccggcg cggactcggt cggggtgacc ttggtcgaga cgcgtgagca cctccagcgt
      481 gtccactccc tcgagccgcc caccttgcgc ccgcccgccg aggcggagcc ggcgctcagg
      541 agctaggacg gccgagcagc tcgtcgagca tggtggcgtc caccggcttc gtcacgtgct
      601 ggtcgaagcc ggcggccagc gcccgccgcc gatcatccgc ttgaccgtag ccggtgaccg
      661 cgacgagccg gatcgcccgc gtgtccggcc gccggcgcag ctcgcgcgcg accgcgtagc
      721 cgtccatgcc gggcaggccc agatcgatca gcgccacgtc gggccggaac gcgcccagct
      781 tggcgaggcc gctcggcccg tcctcggagg tctcgacgtg gtggccggcc atctcgagca
      841 gcaggcgcag gctctggcgc gcgtccgggc tgtcctccac gaccagcacg cgccggggga
      901 cgaccgtcgc gcgcgtcgcc tcctcacttc cggccgcctc gtcggcgggc gccgcgatcg
      961 cgggcaggcg caccgtgaac tcgctgccct gcccaggacc cgcgctccac gccgcgaccg
     1021 cgccgccctg cagctcgacc aggcgcttca cgagcgccag gccgaggccc agcccgccgc
     1081 gcgagcggtc caggctctgc ggctcctgca cgaacaggtc gaacacgcgg ccgagcaggt
     1141 cggtgcggat gccctggccg ctgtcgcgca cccgcagcac cccggtgtcc ccgaccgcct
     1201 cggtggtcac ctggacccgc ccgcccggcg gcgtgtactt gagcgcgttg tcgagcaggt
     1261 tgttgaccac ctgctcgagc cgggcggggt cggcgtgcac gtggacggcc gcgccctcga
     1321 ggcgcacgtc gtgccgctcg gtgcgcccgg cctgggcgag ggcgtcgagg cagcgccgcg
     1381 cgagcgcgtg tagctcgacg gcccgccgct gcaggtcgat cttgcccgac gtgacgcggg
     1441 ccacgtcgag caggtcgtcg accaggcggg cgaggtgacc ggtctgccgc ccgatgatcg
     1501 ccaccacgtg gcgcatcgtc tcgtcgccct ggaggcgctc cagcaccgcg accgcgttgg
     1561 agagcgtgcc gagagggttg cgcagctcgt ggccgagcat ggccaggaac tcgtccttgg
     1621 cctgcgtctc ctcccgcagg gaccgcgcca gggcctcggc ctcctcccgc cgctcgcgca
     1681 cgtgcgcctc gtgctggctg cgctcggtga tgtcgagcga cacgctcagc accgcgggct
     1741 cgccaccgaa ggtgatcacc tccgagttca cgagcagccg gcgctcctcg cccgcgcggg
     1801 tcaggaaggc gacctcgaag tcgcgcacgc gcccctcctg gtggagcagc cccagcatcc
     1861 gctcgcgctg ctcgccggtc acccagaagc cgaggtccat cttgctcttg ccgagcatct
     1921 cctcgcgcga gtacccgccg tgacgggtcg ccgcctcgtt cacctccagg tggcggtggt
     1981 cgcgcaggct ggtgatcgac atcggcaggg ggttggcgtg gaaggccacc atgaagcgcc
     2041 gctcgctctc gcggagcgcc tcgtcggccc gcgtgcggtc ggtgatgtcg gtcagcgcgc
     2101 tcaggatgca cgcatccccg tcgaactcca cgatgtcggc cgagagcacc gtgacgcgaa
     2161 tgtcaccgtt cttgacccgg aagcggcact cgatgctgcg caccgggcgg cccgccttga
     2221 gctcccggat gccgtcgtcg cgctggctcg gatccaccca gaggccgagc tcgaccgggg
     2281 tgcggccgag gatctcctcc cgacggtagc cggtcgtggc caggaatgcg tcgttggcgt
     2341 cgcggatgcg gccgtcgctc acgccggtga ggcagaggat cgtggggctg agcccgaaga
     2401 ccgtgcggag cttttccccg aagcccgggt ccgatggcat ccccggcagt atgccgggcg
     2461 gggccgccgg ctggcaagcc ggccccgcgc ggcggcccgg gttgggtgta tcatcggcgc
     2521 cgacgctgac cctcccagga ggcgaccatg acgagtcagg cgagcgcggg ctacgagatc
     2581 gacgtccagg acgtggagta cctccgtcac ggtgactggc cgctgctggc gcggctctac
     2641 agcccgcgcg gcgacgggcc cttcccgatg atcgtggacc tgcacggcgg ggcctggtgc
     2701 cggggcgacc gcaccaacga cgcggtcatg aacgagcccc tggcccggag cggcgtggtg
     2761 gtggccgcgc tcgacttccg catgccgccc gacgcctcgt atccgggctc cctcgtcgac
     2821 atcagctacg gcatccgctg gctcaagagc cgtgcgacca ccctgcgcgg ccggccggac
     2881 ctggtcggga tcatgggcgc ctcgagcggc gcgcatcagg cgatgctgct cgcgatgaag
     2941 ccgcgcgatc ccaagtacgc gggcctcccc ctgcccgccg gcgtctccgc ggtggacggc
     3001 tcggtccgct gcgcggtgct ctgctggccg gtcatcgacc cgctcgggcg ctaccactat
     3061 gccaagaagc tcaaggccgg gggcgcgccc taccccgagg tggtcgaccg ggtgctgccc
     3121 ggccacgacc agtactggat caccgaggac gcgatggcgg agggcaaccc gaccctcgcg
     3181 ctcgagcgcg gggagtcggt ggagctgcca ccggtgctct acatccaggg cgatcgcgac
     3241 atggcgcacc cgcgcccgga cctcgatcgc ttcgtggcgg gctaccgcaa ggcgggcggc
     3301 gcgctcgacc tcgagctgct ggccggcgag gccgagggct acatcagccg caagccggcc
     3361 tcacccgccg tcgaccggac gatcgcccga atcatcgagt tcgtccacaa acagctcgct
     3421 tagccgccca gcgcgcggcg gactagcggt aggacctcgc gggcgacgcg ggcggtgtag
     3481 tcgcgctcgg ggtcgcccgg ccacttcggg tcggggatcg ggtggaggac cacgtagtcg
     3541 aagggcgcgg gcgcgtcgcg gagggcggtc accagctgcc gcgccacctc ggggggcgcc
     3601 ccgcggaggc tgaaggcatc ggccgcgctc cccgggagga agtccaccgc ccgcccgctc
     3661 gcctccagat ccgtcgcgtg gtgaaaatcg ggccagactc cccgggcgtg cttgaggctc
     3721 tccggatccg ggccactcca ggccagcccc ggcgggtcga agagcgcggg agagtactcg
     3781 tagtatcccg ccgccatcga gcgcgccatg acgagggcgc gctccgcgtc gtccaccagc
     3841 acggtgtgga agacggcgcc gagccgcacc gaggccgggt cgcggccggc cgcgacggcc
     3901 ccggcgcgga tcgcgttcac cgcggtggcg atgttggcgg gatgggtgcc cacccggatg
     3961 aacaccccgt ccgcgacgcc gcccgccatg cgcagggtgc gcgggccgcc ggcggcgatc
     4021 cagatcggca ccggccggtg atgcggcagg cgcgccggcc gcaccgcgcc cacctccacc
     4081 gcctgcccgt cgagcagcgc ccgcatgagg cgcgtgctca cctccagctc gttgacccgc
     4141 gccggcttga ggccggccag gcgcaccgcg gtgtcgccca ctccccagcc gagcagcacc
     4201 cgccccggcg ccagctcgtc cacggtggcg atcgacgacg cggtgacggt gggatggcgg
     4261 ttcaccgggt tggccaggag cggaccgagc acgatccgct cggtgacccg cgccgccgcg
     4321 gccagcagca cgtaggtgtc gcgccggcgg agctgcgagt ccggctggag cgcggcgtcc
     4381 cagccgagct gctcggcgcg gcgcacgtcc gccgcgaagg cgtcgacgga gcgggagtcg
     4441 aagcgattca acccgaagcg cggcagcggg ggcatcgggc tcaggtcgcg tccagcacgg
     4501 agacgatgtc ctagggcatg ccgggcatgt cgacccgggc gtagtagcgc gcatccccga
     4561 gcgcgtcgac ccgctgccag aactcgggca gcgcggccag ccccgtctcg ttgacggcgg
     4621 ccccctggcg ccagatgcac caggagtcgg ggtgcaggcg gctcgcctcg tggatccacc
     4681 gctcaccttc ctcgtgctct ccccggcgga tcaggagctg cccgagccgg aacatcgcct
     4741 gggcccgggc ggtgtcctcg cgcggcgtgg gcaggtgctc gcgcgcctgg gccgcggtga
     4801 gggcgtggga gctcttcggc ccgaggcgca cccagtcgcg gatggcgtcg aggtaggtcg
     4861 ccttcgcctg cgcggtgaga cgcgccacgt cctccggcat ctcccgcgtc gcccggttca
     4921 tcttgcggaa gccttcatag gcgccggccg gctccgcggg acgcacgatg cggccggcct
     4981 cgtcgatcca caccgcctgc ggcacgttga cgatgccgta gagctcggcg agccgatg
//
LOCUS       DATMCV010001113         3783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_329623, whole genome shotgun sequence.
ACCESSION   DATMCV010001113 DATMCV010000000
VERSION     DATMCV010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329623"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VNU03_25460"
     CDS             <1..639
                     /locus_tag="VNU03_25460"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77895.1"
                     /translation="KRALEPLVTLGAPWKILTTGVAVKPYPSCACTHSIIDGVLELRR
                     AQGIRADDVEELTVGVGAGVPRILIHSRPRTGLEAKFSAEFSAAAALADGRVGMATFQ
                     DDRAQDPAIRRLMERVRVVVDPDIPGDLERHMWTRLTVRLKDGRQATIDPRPVPGHPA
                     NPLSLEALREKFRECAGLVLPADRVDTVAQMVESLDTCPDLRSLTAILDPGR"
     gene            complement(650..1432)
                     /locus_tag="VNU03_25465"
     CDS             complement(650..1432)
                     /locus_tag="VNU03_25465"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HWP77896.1"
                     /translation="MRVIWSTLLVYGIAFAAVFGLQLLAVTALLTWRTGTLDLERDAL
                     TALLVGVPASSLTLIAIALLAAGRPRSLRLRLLPSGVSAREIVVMVVGILALSQALES
                     LVLLLDVGTGPALEWMTRTVASATPLGLALAVVVVGLFAPVGEELFFRGYMLTRLRRV
                     WSPGPAILVSAVAFGIIHGEWVHGLLAAAIGIYLGLVTERSGSVVPAIICHAVNNIAS
                     VLLSAWLGSPQGHALNAALVIAAGLVVAWALVRLRRAWPRPA"
     gene            complement(1484..2500)
                     /locus_tag="VNU03_25470"
     CDS             complement(1484..2500)
                     /locus_tag="VNU03_25470"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWP77897.1"
                     /translation="MLIGILIGFPMSAAKSATAPAPGGKGEHATAGHGTGHEIDPRII
                     ARGKATFAQYCANCHGNEGKGDGIAGQNLPIKPQNLTEGRILNALPDHFLHTLIAHGG
                     QAVGLSPLMPNFTPYLSDGQIGEVVAYVRTLAQPAYDPKGVLPVATTRQGPEQPIFFS
                     HVIHAGSYQIACQYCHSGARRSNAAGVPSVERCMGCHKIVAAEGNPEVKKLHGYWDRQ
                     EPIPWVRIFKVPEHAQFTHKQHVQAGVQCQTCHGRIEAMERVHAETGQHILNDLQNLA
                     LMPAPPRKLTMGWCVECHRTANVKGVQGVQTAADAPIPSVWAAPGSESARRNAPLECV
                     TCHH"
     gene            complement(2623..2961)
                     /locus_tag="VNU03_25475"
     CDS             complement(2623..2961)
                     /locus_tag="VNU03_25475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77898.1"
                     /translation="MTGETDSRAGTSCRITLGSRETIVVNHSKGGFQGGILAVEVLTF
                     MGLCSERLFACDLDGPEGRAVLARLTGDGAPAGGAPTPLAAFVRYLEGSASRADLEAR
                     WEALMSGRHQ"
     gene            complement(3045..3356)
                     /locus_tag="VNU03_25480"
     CDS             complement(3045..3356)
                     /locus_tag="VNU03_25480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77899.1"
                     /translation="MTLRRVLILALLVYVALDLSIPTMPGAFGFDPDDSVESVYQTRA
                     RAAAEAVIPPASGAAAFVLSALPPDSGRRPGPIDPARERPRSSRRSAASDEPPPSEDP
                     H"
     gene            complement(3443..>3783)
                     /locus_tag="VNU03_25485"
     CDS             complement(3443..>3783)
                     /locus_tag="VNU03_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWP77900.1"
                     /translation="ILAERRRQKAGTMSGGEQQMVAIGRALMTSPKLIMLDEPSLGLA
                     PKFVSLIFEKLGEMRQAGYTLMVVEQNAAKALSVADRAYVLELGRNRFEGTGRTLLED
                     PEVKRLYLGG"
BASE COUNT          568 a         1357 c         1355 g          503 t
ORIGIN      
        1 aagcgggcgc tcgagccgct cgtcaccctg ggcgcgccgt ggaagatcct gaccaccggg
       61 gtcgcggtga agccctatcc ttcctgcgcc tgcacgcatt ccatcatcga cggcgtgctc
      121 gagctgcgcc gagctcaggg catccgcgcg gacgacgtgg aggagctcac ggtgggcgtg
      181 ggcgcgggcg tcccgcgtat cctgatccac tcgcgcccgc gcaccgggct cgaggcgaag
      241 ttctcggccg agttctcggc cgcggccgcg ctggccgacg gccgggtggg catggcgacc
      301 ttccaggacg accgcgcgca ggatcccgcg atccggcgcc tgatggagcg cgttcgcgtg
      361 gtcgtggatc cggacatccc gggcgatctc gagcgccaca tgtggacgcg gctcaccgtg
      421 cggctcaagg acgggcgcca ggccaccatc gacccgcggc cggtccccgg ccatcccgcg
      481 aatccgctgt cgctcgaggc gctgcgcgag aagttccgcg agtgcgcggg cctcgtgctg
      541 cccgccgacc gcgtcgacac ggtggcccag atggtcgagt ccctcgacac ctgccccgac
      601 ctgcgcagcc tgaccgcgat cctcgatccc ggccgctgag cgcggcccct caggcgggcc
      661 gcggccacgc ccggcgcagc cggacgagcg cccaggcgac cacgagcccg gcggcgatca
      721 cgagcgccgc gttcagcgcg tggccctggg gcgagccgag ccacgccgac agcagcacgg
      781 aggcgatgtt gttgaccgcg tggcagatga tcgcgggcac cacgctgccg gagcgctcgg
      841 tcacgagccc caggtagatg ccgatggcgg cggcgagcag cccgtgcacc cactcgccgt
      901 ggatgatgcc gaaggcgacc gcgctcacca ggatggccgg acccgggctc cagacccgac
      961 gcaggcgcgt cagcatgtag ccccggaaga acagctcctc gccgaccggc gcgaacaggc
     1021 cgaccaccac caccgcgagg gcgagcccga ggggcgtggc ggacgccacg gtgcgcgtca
     1081 tccactcgag cgcggggccg gtgcccacgt ccagcaggag caccagcgac tcgagcgcct
     1141 gactcagcgc gaggatgccg accaccatca ccacgatctc gcgcgcggag accccgctcg
     1201 gcaggaggcg caggcgaagg ctccgcgggc ggccggcggc cagcagcgcg atggcgatca
     1261 aggtgagcga ggaggcgggc accccgacca gcagcgcggt gagggcgtcg cgctcgagat
     1321 ccagggtgcc ggtgcgccac gtcaggagcg cggtcacggc caggagctgc agcccgaaca
     1381 ccgcggcgaa ggcgatcccg tagacgagca gcgtgctcca gatcacgcgc atcggcgccc
     1441 caccccgacc gcggcccgcg aggcgcgggc gcggtgggtc aggctaatgg tggcaggtca
     1501 cgcactcgag cggcgcgttg cggcgcgcgc tctcgctgcc cggagccgcc cagaccgagg
     1561 ggatcggcgc gtccgccgcc gtctgcacgc cctgcacgcc cttgacgttc gcggtccggt
     1621 ggcactccac gcaccagccc atcgtgagct tgcgcggcgg cgccggcatc agggcgagat
     1681 tctgcaggtc gttcaggatg tgctggccgg tctcggcgtg cacccgctcc atcgcctcga
     1741 tgcggccgtg gcaggtctgg cactgcacgc ccgcctgcac gtgctgcttg tgggtgaact
     1801 gggcgtgctc gggcaccttg aagatgcgca cccacgggat gggctcctgc cgatcccagt
     1861 agccgtggag cttcttgacc tcggggttgc cctcggcggc cacgatcttg tggcagccca
     1921 tgcagcgctc caccgacggc acgcccgccg cgttgctccg gcgcgcgccg gagtgacagt
     1981 actggcaggc gatctggtac gagccggcgt ggatcacgtg gctgaagaag atcggctgct
     2041 ccggcccctg gcgggtggtg gccaccggca ggacgccctt ggggtcgtag gccggctggg
     2101 ccagcgtccg gacgtaggcg acgacctcgc cgatctgccc gtccgagagg tacggggtga
     2161 agttcggcat gaggggcgag agaccgaccg cctggccgcc gtgagcgatc agggtatgca
     2221 ggaaatgatc cggcagcgcg ttcaggatcc gcccctcggt caggttctgg ggcttgatgg
     2281 gcaggttctg ccccgcgatg ccgtcgccct tgccctcgtt gccgtgacag ttggcacagt
     2341 actgggcgaa ggtcgccttg ccgcgggcga tgatccgggg gtcgatctcg tgaccggtgc
     2401 cgtgcccggc ggtcgcgtgc tcacccttgc cacccggggc cggggcggtc gcggacttgg
     2461 cggcggacat cgggaatccg atcaggattc cgatcagcac ggccgcgatg gccgcactac
     2521 cgacgacggc tgatcgtggg atacgcatga ggttcggcct gtctcccttg gaaaacccca
     2581 ccgaatatac tcaaaccgcg gccggaagca tagacgaaaa aatcattggt ggcgacccga
     2641 catgagggcc tcccaccggg cctcgaggtc ggcccgcgag gccgagccct cgagatagcg
     2701 cacgaaggcg gccagcgggg tcggcgcgcc gcccgcgggg gcgccgtcac cggtcaaccg
     2761 ggcgaggacg gcccggccct ccggcccatc cagatcgcag gcgaagagac gctcggagca
     2821 cagccccatg aaggtcagga cctccaccgc gaggatcccg ccttgaaacc ctcccttgct
     2881 gtgattgacc acgatcgtct ccctgctccc cagggtgatc cggcacgatg tgccggccct
     2941 cgaatcggtc tcccccgtca tggacagtcc tcccgcagag cacacgtgtt ggaagcggcc
     3001 gggccgcccc ggaagggcgg tcggttcaaa cgcgagcggg agggctagtg cgggtcttcg
     3061 gagggaggcg gctcgtcgga ggccgcagat cgtcggctcg agcgtgggcg ctcccgggcc
     3121 gggtcgatcg ggccaggccg gcggccggaa tcgggcggca gcgcggacag cacgaacgcg
     3181 gcggcccccg acgcgggagg gatcaccgcc tcggcggcgg cccgggcgcg ggtctggtag
     3241 acgctctcga ccgaatcgtc cggatcgaag ccgaacgctc ccggcatcgt ggggatcgac
     3301 aggtcgagcg ccacgtacac caggagggcc aggatcagca cgcgccggag cgtcacggcc
     3361 gcgattctac ccgaatcccg ggccgaatcg agggccggtc gggcggcctc ccggatcaac
     3421 cgccccgctc ggcctccccc gctcagcctc caagatagag gcgcttgacc tcggggtcct
     3481 ccagcagggt gcgccccgtc ccctcgaacc gattgcggcc cagctcgagc acgtacgcgc
     3541 gatccgccac cgagagggcc ttggccgcgt tctgctccac caccatcagg gtgtagccgg
     3601 cctgccgcat ctcgcccagc ttctcgaaga tcagcgagac gaacttcggc gcgaggccga
     3661 gcgacggctc gtcgagcatg atcagcttcg ggctggtcat cagcgcgcgc ccgatcgcca
     3721 ccatctgctg ctcgcctccc gacatggtgc cggccttctg gcgccgccgc tcggccagga
     3781 tcg
//
LOCUS       DATMCV010001114         3346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_60079, whole genome shotgun sequence.
ACCESSION   DATMCV010001114 DATMCV010000000
VERSION     DATMCV010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60079"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VNU03_25490"
     CDS             <1..488
                     /locus_tag="VNU03_25490"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP77901.1"
                     /translation="ETKDRKYLTIGCTEPWLWENFCKAINRPDMVKFARKADQFVRAA
                     NAEEVKAREEIEAIIRTRTRDEWYDFLVKADVCVGKVYEPEEVVADPQVQARDMIVEM
                     QHPVHGTVKQFGQPIKLSDTPGTMRTAAPYSGEHTDQVLGELGLPAAEIRSLREKKIV
                     A"
     gene            525..1235
                     /locus_tag="VNU03_25495"
     CDS             525..1235
                     /locus_tag="VNU03_25495"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HWP77902.1"
                     /translation="MTTIRRGIVLAALGLLATAAGARADSPGELGERLRKATAVLSEM
                     ASGKQDTDIPREMLARARAIAVFPGVKKGAVIVGGRHGQGVMSVKRVSDGRWSAPAFF
                     TVGGGSFGLQLGGQVIDLVLVVMTEKGIESLLKSETTLGGDVSLTAGPKSLHEAANTD
                     GSFKAEIFSYARSSGFFAGASFEGATVQPDGSAIRTLYGAKADSRDVLLNGRYAVPAS
                     ARPFVNVLTTYSPPPKKR"
     gene            1246..2451
                     /locus_tag="VNU03_25500"
     CDS             1246..2451
                     /locus_tag="VNU03_25500"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWP77903.1"
                     /translation="MTTAPESFVELLSTRARIGWGAPPTARPVTNALYEFGGGLPDPA
                     SFPYDGLVDATSRMIKAEGAEALTYGEPLGYRGLRELVCHKYGLFEGLKVGPENIIIS
                     NGSGHALSLAFSAFVDPGDVILSEAPTFSGTLQTIRRHGPQVLDVPVDKDGLVVEVAR
                     EHLATLKKQGRRCKLIYTIVNFQNPSGPSLTLRRRRELIDLAHEYDTFILEDDAYGEL
                     RFEGEHLPSLYALDGGGRVMRAGTVSKILGAGFRLGWLCAPKEMIPAFQGFLFGGGVN
                     PFASRVATYFLRDNLAPHVALLVNVYRAKRDAMLRGLWEVLKDTDVEISKPEGGFFIW
                     IKLPTGTDTGKLYDEAVKARVQYTAGPSFFPNGGGERFIRLAFSFESPERCYEGARLI
                     ARAIVDART"
     gene            2453..2848
                     /locus_tag="VNU03_25505"
     CDS             2453..2848
                     /locus_tag="VNU03_25505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77904.1"
                     /translation="MARHLILVVDDEDNLRDVLVEVLKRDGHEVDSAVDGAEGLRRAQ
                     ERRYDLVVTDLRMPGLEGPELYRALRERYPDRPPRVIFMSANTGIDEYATFLAETGEP
                     ALEKPFNLTDMRQVVLQVLTQVRRAAGTE"
BASE COUNT          512 a         1197 c         1147 g          490 t
ORIGIN      
        1 acgagaccaa ggaccgcaag tatctgacca tcgggtgcac cgagccctgg ctgtgggaga
       61 acttctgcaa ggcgatcaac cggccggaca tggtgaagtt cgcccgcaag gccgatcagt
      121 tcgtgcgagc ggccaacgcg gaagaggtga aggcgcgcga ggagatcgag gccatcatcc
      181 ggacccgcac ccgcgacgag tggtacgact tcctggtcaa ggccgacgtc tgcgtgggca
      241 aggtctacga gccggaagag gtggtcgccg atccgcaggt ccaggcccgc gacatgatcg
      301 tggagatgca gcacccggta cacgggaccg tgaagcagtt cggccagccc atcaagctct
      361 ccgacacccc ggggaccatg cggaccgcgg cgccctactc gggtgagcac accgatcagg
      421 tgctgggcga gctcggcctg cccgcggcgg agatccggag cctgcgcgag aagaagatcg
      481 tcgcctagcc cgccgaacgg gaaatgggct aatatcgtca tctgatgacg acgatccgtc
      541 ggggtatcgt cttggccgcg ctgggcctgc tggcgacggc ggccggggcg agggccgaca
      601 gccccggcga gctcggcgag cggctgcgca aggcgaccgc ggtgctctcc gagatggcca
      661 gcggcaagca ggacaccgac attccgcgcg agatgctcgc ccgggcgcgt gcgatcgcgg
      721 tcttcccggg ggtcaagaag ggcgcggtca tcgtcggcgg ccgccacggg cagggcgtca
      781 tgagcgtgaa gcgggtctcg gatgggcgct ggagcgcgcc cgccttcttc accgtcgggg
      841 gcggcagctt cggccttcag ctcggtggcc aggtcatcga cctggtgctc gtggtcatga
      901 ccgagaaggg catcgagagc ctgctcaaga gcgagaccac cctcggcggc gacgtctcgc
      961 tcaccgccgg tcccaagagc ctgcacgagg cggccaacac cgacggctcg ttcaaggccg
     1021 agatcttctc ctatgcgcgg tccagcggct tcttcgcggg cgcctcgttc gagggcgcca
     1081 ccgtgcagcc ggacggctcc gcgatccgca ccctctacgg ggcgaaggcc gactcccgcg
     1141 acgtgctgct caacggtcgc tacgcagtcc cggccagcgc ccgccccttc gtcaacgtgc
     1201 tgaccaccta ctcgccaccg cccaagaagc gctgagagcc atccgatgac cactgccccc
     1261 gaatccttcg tcgagctcct gtccacccgc gcccgcatcg gctggggcgc gccgcccacc
     1321 gcgcgcccgg tcaccaacgc gctctacgag ttcggcggcg gcttgccgga cccggcgtcc
     1381 ttcccgtacg acgggctggt cgacgccact tcgcggatga taaaggccga aggcgcggag
     1441 gccctgacct atggcgagcc gctgggctac cgcggcctgc gtgagctggt gtgccacaag
     1501 tacgggctct tcgagggact caaggtcggc cccgagaaca tcatcatctc gaacggctcg
     1561 gggcacgccc tctcgctcgc cttcagcgcc ttcgtggacc cgggcgacgt gatcctctcg
     1621 gaggcgccca ccttctcggg cacgctccag accatccgcc gccacggccc ccaggtgctc
     1681 gacgtgccgg tcgataagga cggcctggtg gtcgaggtcg cgcgcgagca tctcgccacc
     1741 ctcaagaagc agggccggcg ctgcaagctc atctacacga tcgtgaactt ccagaacccg
     1801 tccgggccct cgctcaccct gcgccggcgc cgggagctga tcgacctggc ccacgagtac
     1861 gacacgttca tcctcgagga cgacgcctac ggcgagctgc gcttcgaggg cgagcacctc
     1921 ccgtcgctct acgccctcga cggcgggggc cgggtgatgc gcgcgggcac cgtgtccaag
     1981 atcctcggcg cgggcttccg cctcggctgg ctgtgcgcgc ccaaggagat gatccccgcg
     2041 ttccaggggt tcctcttcgg cggcggcgtc aatcccttcg cctcgcgcgt ggccacctac
     2101 ttcctgcgcg acaacctcgc tccccacgtg gccctgctgg tcaacgtgta ccgcgccaag
     2161 cgggacgcga tgctgcgcgg gctctgggag gtcctgaagg acaccgacgt cgagatcagc
     2221 aagccggagg gcggcttctt catctggatc aagctcccca ccggcaccga caccggcaag
     2281 ctctacgacg aggcggtgaa ggcccgcgta cagtacaccg cggggccgtc gttcttcccc
     2341 aacgggggcg gcgagcgctt catccgcctg gccttcagct tcgagtcccc cgagcgctgc
     2401 tacgagggcg cacgcctgat cgcccgggcc atcgtcgacg cccgcacctg agatggcccg
     2461 ccacctgatc ctcgtggtgg acgacgagga caacctccgc gacgtcctgg tggaggtgct
     2521 gaagcgcgac gggcacgagg tggacagcgc ggtcgacggc gccgagggcc tgcgccgggc
     2581 ccaggagcgc cgctacgacc tggtcgtcac cgacctgcgc atgcccgggc tcgaggggcc
     2641 ggagctgtac cgggccctgc gcgagcgcta cccggatcgc ccgccccgcg tcatcttcat
     2701 gagcgccaac accggcatcg acgagtacgc gaccttcctc gccgagaccg gggagccggc
     2761 gctggagaag ccgttcaacc tgacggatat gcggcaggtc gtgctgcagg tcctcacgca
     2821 ggttcgtcgc gcggcgggga ctgagtaggc gccgggcggg agcgggagcc gcggcgggcg
     2881 cggcgggggg cctcccgggg acacgctgac tcgcggcccg cgtgggcact ctatcgagcc
     2941 acgagaccga ggcgttcatg cttcgtcatc gcgcagaaga cacggtcccc gggaggcccc
     3001 ccgccgcgcc cgccgcggct cccgctcccg ccctcccgct cgcttctggt acagccgcac
     3061 attcctacgg gcatgatgat tcgctgagcg gttgacgtgg gcggcgggcg ctgatagcgt
     3121 gcccgggtgg caaccgccag cccggccctc gtcgatcctt cacgctctac cccggacgtc
     3181 gccgagcaac gtgcgcgagt ccgtgccatc gtcgcgccgc gcagtggtga gcgtggtctg
     3241 gacatcggct gggcttcggg ctgctctcct gcgagctggc ccgcgacgtg ggaccgaccg
     3301 ggcgcgtcat gggcgtcgac tcggtcccgg ccatgatcgt ggcctg
//
LOCUS       DATMCV010001115         2878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_132290, whole genome shotgun sequence.
ACCESSION   DATMCV010001115 DATMCV010000000
VERSION     DATMCV010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132290"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1279
                     /locus_tag="VNU03_25510"
     CDS             329..1279
                     /locus_tag="VNU03_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWP77905.1"
                     /translation="MTTETSEQLLREMVRIRRIEETLAELYPSQEMRTPTHFSIGQEA
                     VAVGVCSALRRTDAVYSGHRCHAHYLAKGGDLAGMVAELYGRETGCARGRGGSVHLND
                     PDAGVVASSAILGQTMAVAVGTALAFAMDGRDDVAVTFFGDGTVEEGIFHESLNFAVM
                     RRAPVLFVCENNGFSTHTRLDVRQPAAVPIHVRGGSYGMPSRLVDGNDVFAVHEAARE
                     AVAHCRRGAGPFFLECTTYRWREHVGPLWDYDKGYRSKAEVDSWIARCPIRRAAERLA
                     TTPARLAALERESQVEVDAAVAAARIAPFPAVEDLTLGTY"
     gene            1288..2322
                     /locus_tag="VNU03_25515"
     CDS             1288..2322
                     /locus_tag="VNU03_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWP77906.1"
                     /translation="MRRLSYGQAISEATVQCMEADPSIFVTGIGVDDFKGIFGTTAEA
                     FRRFGPARVFDIPNGENAMTGIAIGAAAAGKRPLVVHPRNDFMFLCMDQLFNLAAKWR
                     YMFGNRSGVPVVFRGIVGRGWGQGATHSQSLQALFAHFPGLYVGLPASPADAKGLLVS
                     ALQGSGPVVLLENRALYELEGDVPETLEAIPFGKGRVVRPGTDVTIVGISLMAYEAAR
                     AAEILVDHNVSAEVIDLRSVRPLDEEIILRSLRKTGRLVVADTSWALCGVSAEVAAIA
                     AEKGWHYLKAPVRRVTPPDCPAPVSKPLEDAFHPNPMTIAQACLDVLKAGAPLGTGVA
                     DVQASFAGPY"
     gene            2324..>2878
                     /locus_tag="VNU03_25520"
     CDS             2324..>2878
                     /locus_tag="VNU03_25520"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWP77907.1"
                     /translation="MESRRRVSVFLPALNEVDNLEGAVADIVWAASEVLTDYEILIID
                     DGSTDGTGPLADRLARENPRIRAIHQPTNMGIAAAYERALGEARLDYFSFLAADGEIA
                     RESVRDILGSVGRADIVAPYHQNPRARQAHRRFLTWASTALVNALFRQRMHYYQGPCI
                     YPVPLARALPKSAGGFYFLTQMLIH"
BASE COUNT          422 a         1008 c         1008 g          440 t
ORIGIN      
        1 gcggatccgg ctgacatggc ggcaattcta gcaagggctc ggcgcgccgt ggagccgttt
       61 gcacggggcc ggcggtgcgg tgtccttggc atggccgatg caagttgtgc gtgctaagcg
      121 accgcccgcc tcggaagatc ccgtgctgtc ggggtgggga ggtgatccac aatacaccgt
      181 ttatattgac gtttcaggtt agcggcgggt cgtgttcgaa ccgcctccga ccctcgacag
      241 cgagcgtgcg agtgaccaag agcgtcgtca gcgagacgct gctcgtaagc tggacagtgc
      301 ccattcacgc catcggcagg cgaggacgat gacgacagag acgagcgagc aactgctgcg
      361 cgaaatggtc cggatccggc ggatcgagga gacgctggcc gagctctacc cgtcgcagga
      421 gatgcgcacg cccacccact tctccatcgg gcaggaggcg gtggcggtcg gcgtgtgctc
      481 ggcgctgcga cggaccgacg cggtctacag cggccatcgg tgccacgcgc actatctcgc
      541 gaagggcggt gacctcgccg gaatggtggc cgagctctac ggacgcgaga ccggatgcgc
      601 gcgcgggcgc ggcggctccg ttcacctcaa cgatcccgac gcgggtgtcg tcgcttcgtc
      661 ggcgattctc gggcagacca tggcggtggc cgtgggcacc gcgctcgcct tcgcgatgga
      721 cggccgcgac gacgtcgcgg tcaccttctt cggcgacggc accgtcgagg agggcatctt
      781 ccacgagagc ctgaacttcg cggtgatgcg tcgggcgccg gtgctcttcg tctgcgagaa
      841 caacggcttc tcgacccaca cccgcctcga cgtgcgccag ccggccgcgg tgccgatcca
      901 cgtgcgcggc ggctcctacg gcatgccctc gcgcctggtc gacggcaacg acgtgttcgc
      961 ggtccacgaa gcggcgcgcg aggcggtggc ccactgccgt cgcggcgcgg gtcccttctt
     1021 ccttgagtgc acgacctacc gctggcggga gcacgtgggg ccgctctggg actacgacaa
     1081 gggctaccgg tccaaggccg aggtcgactc gtggatcgct cgctgcccga tccgccgggc
     1141 cgccgagcgg ctcgcgacca ctcccgcgcg gctggccgcg ctcgagcgcg agagccaggt
     1201 cgaggtcgac gcggcggtcg cggcggcgcg gatcgcaccc ttcccggcgg tggaagacct
     1261 caccctgggg acgtactgag gccgacgatg aggcgactga gctacgggca ggcgatcagc
     1321 gaggccaccg tgcagtgcat ggaggcggat cccagcattt tcgtcaccgg catcggcgtc
     1381 gacgacttca aggggatctt cggcaccacc gcggaggcgt tccgtcgctt cggtcccgcg
     1441 cgggtcttcg acatccccaa cggcgagaac gcgatgaccg gaatcgccat cggggcggcc
     1501 gcggccggga agcggccgct ggtggtccat ccgcggaacg acttcatgtt cctgtgcatg
     1561 gaccagctct tcaacctcgc cgccaagtgg cgctacatgt tcggcaaccg cagcggggtg
     1621 ccggtggtct tccgcgggat cgtgggacgg ggctgggggc agggggccac gcactcccag
     1681 agcctgcagg ccctcttcgc ccacttcccc ggcctctacg tgggcctccc cgcctctccc
     1741 gccgacgcca agggcctcct ggtgagcgcc ctgcagggga gcggcccggt ggttctcctg
     1801 gagaaccgcg cgctctacga gctcgagggg gacgtgccgg agacgctcga ggcgatcccc
     1861 ttcggcaagg ggcgggtggt ccggcccggc accgacgtga ccatcgtcgg gatctccctc
     1921 atggcctacg aggcggcccg cgccgccgag atcctggtgg accacaacgt gagcgccgag
     1981 gtcatcgacc tgcgcagcgt ccgtccgctc gacgaggaga tcatcctgcg ctcgctccgg
     2041 aagacgggcc gcctcgtggt ggctgacacc agctgggcgc tctgcggagt ctccgccgag
     2101 gtggccgcga tcgcggccga gaagggctgg cactacctca aggctccggt gcgccgggtc
     2161 acgccgcccg actgcccggc gccggtgtcg aagccgctcg aggacgcctt ccatccgaac
     2221 ccgatgacga tcgcgcaagc ctgcctcgac gtcctcaagg cgggcgcacc gctcgggacc
     2281 ggggtcgcgg acgtgcaggc ctccttcgcg gggccctact gagatggaga gccggcgtcg
     2341 cgtcagcgtg ttcctgcccg cgctcaacga ggtcgacaac ctcgagggcg cggtggccga
     2401 catcgtgtgg gccgcgtccg aggtcctcac cgactacgag atcctcatca tcgacgacgg
     2461 cagcaccgac gggaccggcc ctctcgccga tcggctcgcc cgtgagaacc cgcggatccg
     2521 ggcgattcac cagccgacga acatggggat cgcggccgcc tacgagcggg cgctgggtga
     2581 ggcccggctc gactacttca gcttcctcgc cgccgacggc gagatcgcgc gcgagtcggt
     2641 gcgggacatc ctgggctcgg tgggccgggc cgacatcgtc gccccctatc accagaatcc
     2701 ccgggcccgc caggcccatc gccggttcct gacctgggcg tcgaccgccc tcgtcaacgc
     2761 gctgttccgg cagcgcatgc actactacca gggtccgtgc atttacccgg tgcccctggc
     2821 ccgggccctg cccaagagcg cgggtggctt ctacttcctg acccagatgc tcatccac
//
LOCUS       DATMCV010001116         2154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_60356, whole genome shotgun sequence.
ACCESSION   DATMCV010001116 DATMCV010000000
VERSION     DATMCV010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60356"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VNU03_25525"
     CDS             <1..926
                     /locus_tag="VNU03_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWP77908.1"
                     /translation="SLRLAPGDELLTTDHAYQACRNTLDFVAERSGARVVTVTLPFPV
                     ASGQAIVDTVMAGVTPRTRLALLDHITSPTALILPIGPLVAALANRGIESVVDGAHAP
                     GMVPLDLRALGAAYYSGNCHKWLCTPKGSAFLHVRRDRQADIHPLTISHGAVGERPGR
                     TRFRLEFDWTGTQDPTAWLTVPMAIDYLAALVPGGWPALMARNRALALEARRLLCEAV
                     GTAPACPDEMIGSIASVILPNNRTVETRWRVRDPLQGRLFEDWGIEVPIMRWPAPPRR
                     LIRVSAQLYNRLPQYTRLAEALRKELPAELG"
     gene            complement(923..1231)
                     /locus_tag="VNU03_25530"
     CDS             complement(923..1231)
                     /locus_tag="VNU03_25530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWP77909.1"
                     /translation="MADVLTVVAKIRAAKGKGDALAALLKEQAGVVRKAEPGCLAYRP
                     HRSAKDPDLFIFYEQYKDDAAFDLHRKAPHLAAYRERREKEGLTEGPAEVEIYRSLTD
                     "
     gene            complement(1273..>2154)
                     /locus_tag="VNU03_25535"
     CDS             complement(1273..>2154)
                     /locus_tag="VNU03_25535"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77910.1"
                     /translation="LALAALSNTFRAFDEPSRMSLVPQMVARERLPNAIALGSIPWQA
                     GRMIGPSITGVLIAAFGGTVGFGLAAASSVLALVMYSRLRVSADAPSPSGQHVLRQLY
                     AGLHFVAHDFVFAALICLALFNALFGLSYVTLLPIFSDVYFQTGSAGYGLLNAAHGIG
                     ALAGTLIVASLAARFRRRGLVILTGAAGLGLGLVIFSRAPGLGLALPMLACAGFSNTF
                     YLTQVSTHLQQAVPDHLRGRVMSLYSLCWNLLPLGGLIGGVLAAAVDARFAVTVGGAL
                     VAANALVLLASRRLRLL"
BASE COUNT          313 a          794 c          772 g          275 t
ORIGIN      
        1 gctcgctgcg cctcgctccg ggcgacgagc tgctcaccac cgaccacgcc taccaggcct
       61 gccgcaacac gctcgacttc gtggccgagc gcagcggcgc gcgggtcgtc acggtcacgc
      121 taccgttccc ggtggcgtcc gggcaggcga tcgtcgacac cgtgatggcc ggggtcacgc
      181 cacgcacccg cctcgccctg ctcgaccaca tcacgagccc gaccgccctc atcctgccga
      241 tcgggccgct ggtggcggcc ctcgccaatc gtgggatcga gtccgtggtg gacggcgccc
      301 acgcgccggg catggtgccg ctcgacctcc gcgcgctcgg cgcggcctac tacagcggca
      361 actgccacaa gtggctctgc acgcccaagg gctccgcctt cctccacgtg cgccgcgatc
      421 gccaggccga catccacccc ctgaccatca gccacggcgc ggtgggggag cggccggggc
      481 gcacccgctt ccggctcgag ttcgactgga ccggcaccca ggacccgacc gcctggctca
      541 ccgtgccgat ggccatcgac tacctggctg ctctcgtgcc cgggggctgg cccgcgctga
      601 tggcccgcaa ccgcgcgctc gcgctcgagg cgcgccggct cctgtgcgag gcggtgggca
      661 ccgcgcccgc gtgccccgac gagatgatcg gctcgatcgc gagcgtgatc ctgccgaaca
      721 accggacggt ggagacccgc tggcgggtcc gcgaccccct ccaggggcgg ctcttcgagg
      781 actggggcat cgaggtgccg atcatgcgct ggcccgctcc gccccggcgc ctgatccgcg
      841 tctccgccca gctctacaac cggctcccgc agtacacccg cctcgccgag gccctgcgca
      901 aggagctccc cgccgagctc ggctagtcgg tgagcgagcg gtagatctcg acctcggccg
      961 gcccctcggt gaggccctcc ttctcccgcc gctcgcgata ggcggccagg tgcggcgcct
     1021 tgcggtgcag gtcgaaggcc gcgtcgtcct tgtactgctc gtaaaagatg aagagatcgg
     1081 gatcctttgc cgagcggtgc ggccggtagg cgaggcagcc cggctccgcc ttgcgcacca
     1141 ctcccgcctg ctccttgagc agcgcggcga gcgcgtcgcc cttgcccttc gccgcccgga
     1201 tcttcgccac caccgtgagc acgtcggcca tgactgtcgt ctcctttgag ggttgtggct
     1261 gcctcgggcc gactatagca gccggagtcg ccgggaggcg aggaggacca gcgcgttggc
     1321 cgcgaccagg gcgccgccca ccgtcaccgc gaagcgcgcg tcgaccgcgg cggcgagcac
     1381 cccgccgatc aggccgccga gcgggagcag gttccagcag agcgagtaga ggctcatcac
     1441 ccggccgcgc aggtgatccg gcaccgcctg ctggagatgg gtgctcacct gggtcaggta
     1501 gaacgtgttg ctgaagccgg cgcacgccag catcggcagc gcgagtccga gccccggcgc
     1561 ccgcgagaag atgacgagcc ccaggccgag gcccgccgcg ccggtgagga tcacgagccc
     1621 gcggcgccgg aagcgcgcgg cgaggctggc cacgatcagg gtccccgcga gggcgccgat
     1681 gccgtgggcc gcgttgagca gcccgtagcc cgcggagccg gtctggaagt agacgtcgga
     1741 gaagatcggc agcagggtca cgtaggagag gccgaagagc gcgttgaaga gcgccaggca
     1801 gatgagcgcc gcgaagacga agtcgtgggc cacgaagtgg aggcccgcgt agagctggcg
     1861 caggacgtgc tggccgctgg gcgacggggc gtcggcgctc acgcgcagcc ggctgtacat
     1921 gacgagcgcg agaaccgacg aggcggcggc cagcccgaag cccacggtgc cgccgaaggc
     1981 cgcgatcagc accccggtga tcgacgggcc gatcatgcgg ccggcctgcc aggggatgga
     2041 gcccagcgcg atggcgttcg gcagccgctc gcgcgcgacc atctgcggca ccagcgacat
     2101 gcggctcggc tcgtcgaagg cgcggaaggt gttgctgagc gcggccagcg cgag
//
LOCUS       DATMCV010001117         3367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_132422, whole genome shotgun sequence.
ACCESSION   DATMCV010001117 DATMCV010000000
VERSION     DATMCV010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132422"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VNU03_25540"
     CDS             complement(<1..328)
                     /locus_tag="VNU03_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HWP77911.1"
                     /translation="MTPLETLRGKAARCTACPLYRHATQTVFGEGDAPARIVLVGEQP
                     GHEEDLAGRPFVGPAGRLLDRALAGAGLDRSRLYVTNAVKHFKWKKDRSGGKRRLHER
                     PAQSEVE"
     gene            474..1454
                     /locus_tag="VNU03_25545"
     CDS             474..1454
                     /locus_tag="VNU03_25545"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HWP77912.1"
                     /translation="MKQLIVLGGPIHPDGMKQLEAEATVVVTDATSEAGMIAAAKDAE
                     GILFRSKPDCTRTLMAACPKLKVVGRHGVGLDIVDLPAATDLGVAVVHAPGSNSNSVA
                     EHAIMLMLACAKQAVVVDQRTRKADWGKGRWQGLFEMNGKTLGIIGVGNIGRRVAKTA
                     GALGMRVIGYDKFVPPDELRNRGVEPMPDMASVLRQADVVTCHTPHTKETHHMIDAAA
                     VAQMKDGVIFINTSRGKTQDENALVAGLESGKIRAAGIDVFEEEPASSDNRFFQLENV
                     IVSPHIAGVTRESTRNTALQVTAEMLRVLRGEKPHVLGNPDLWPRLGHLK"
     gene            1475..2263
                     /locus_tag="VNU03_25550"
     CDS             1475..2263
                     /locus_tag="VNU03_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HWP77913.1"
                     /translation="MPIDARLPKEIRADIRAGRLNAVTAGLAPGYVQANLAVLPKEQA
                     YDFLLFCQRNPRPCPLIEVTDVGSPEPVGVAPGADLRTDLPRYRIYKDGVLADEVTDV
                     RPFWRDDLVAFLLGCSFTFEWALLDAGIPMRHIEQGKNVAMWKTSVQCRPAGQFHGPM
                     VVSMRPIPAGLLSKAVTASARFPGAHGAPVHMGDPAALGIADVNTPDWGDSVGVRAGE
                     VPVFWACGVTPQAVALASKPAFMITHSPGHMFITDLPNHALAAI"
     gene            2277..3014
                     /gene="pxpB"
                     /locus_tag="VNU03_25555"
     CDS             2277..3014
                     /gene="pxpB"
                     /locus_tag="VNU03_25555"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HWP77914.1"
                     /translation="MTIWPAPRLLPCGDRAVSVELGDEVNRELNARVLALEYLLQQRR
                     LPGVGETVPSYRALLVYYDPLVVDYDTLGVTLRELCERARPEVLPPARTVELPCCYEG
                     ELGFELEAAAEKLGLVPAEVVRLHAAADYYVYFVGFTPGLPYMTGMPDRLTIPRLQNP
                     RTKTPPGSVSIGGVQCCIYSVESPGGFWVLGRTPVRLYDPDAPDPILLRAGDHVRFHA
                     IDRAEYDAIAARVETGRYAPRITGAAS"
     gene            3011..>3367
                     /locus_tag="VNU03_25560"
     CDS             3011..>3367
                     /locus_tag="VNU03_25560"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HWP77915.1"
                     /translation="MSDLLVQEPGPLTTIQDLGRFGHLRVGIPTSGPMDRDAFVLANR
                     LVGNPDGAAALECTLIGARVELTDDRLVAVTGADMSPTVNGATVPAWQAVAVKAGDVL
                     RLGPSRGGVRAYLAVAG"
BASE COUNT          470 a         1240 c         1194 g          463 t
ORIGIN      
        1 cctcgacctc gctctgggcc gggcgttcgt ggaggcgtcg cttcccgccg ctccggtcct
       61 tcttccactt gaagtgcttg accgcgttgg tgacgtagag ccgggagcgg tcgagcccgg
      121 caccggcgag cgcccggtcc agcagccggc ccgccggccc cacgaacggc cggccggcga
      181 ggtcctcctc gtgaccgggc tgctcgccca ccaggacgat gcgcgcgggc gcatccccct
      241 cgccgaagac ggtctgcgtc gcgtgccggt agagcgggca cgcggtgcat ctggcggcct
      301 tcccgcggag cgtctcgagc ggcgtcatgc cgtcctgccg gggcacgccg cgtgccagcc
      361 ggatcggccg tgcaagaggc gtccggcgcc cgcattcccg cggcgggcgc gggtcgaggc
      421 ggcgcgggcc ggcgggatga ctcggtaagg gttactccgc tataatgcgg gccgtgaaac
      481 aactcatcgt gctcggcggg ccgattcacc cggacgggat gaagcagctg gaggcggagg
      541 ccaccgtggt cgtgaccgac gccaccagcg aggcggggat gatcgccgcg gccaaggacg
      601 cggaggggat cctgttccgc tccaagccgg attgcacgcg caccctgatg gcggcctgcc
      661 cgaagctcaa ggtggtggga cggcacggcg tcggcctcga catcgtggac ctgcccgccg
      721 ccaccgacct cggcgtcgcg gtggtgcacg cgcccggctc caactcgaac tcggtggccg
      781 agcacgcgat catgctgatg ctggcgtgcg ccaagcaggc ggtggtggtg gaccagcgca
      841 cccgcaaggc cgactggggc aagggccgct ggcagggcct cttcgagatg aacggcaaga
      901 ccctcggcat catcggggtc ggcaacatcg gccgccgggt ggccaagacc gcgggggcgc
      961 tcggcatgcg ggtgatcggc tacgacaagt tcgtgccccc cgacgagctg cgcaaccgcg
     1021 gcgtcgagcc catgccggac atggcctccg tgctccgcca ggccgacgtg gtgacctgcc
     1081 acaccccgca caccaaagag acgcaccaca tgatcgacgc ggccgccgtc gcccagatga
     1141 aggacggggt gatcttcatc aacacgtccc ggggaaagac ccaggacgag aacgcgctcg
     1201 tggccggact cgagagcggg aagatccgcg ccgcgggcat cgacgtcttc gaggaggagc
     1261 ccgcgtcctc cgacaaccgc ttcttccagc tcgagaacgt gatcgtctcc ccgcacatcg
     1321 cgggggtgac ccgggagagc acgcggaaca ccgcgctcca ggtgaccgcg gagatgctcc
     1381 gcgtgctgcg cggcgagaag ccccacgtcc tcggcaaccc cgatctctgg ccgaggctcg
     1441 gccacctcaa gtagaccgac ccggagaacg aatcatgccc atcgacgccc gccttccgaa
     1501 ggagatccgc gccgacatcc gtgccggacg cctgaacgcg gtgaccgccg ggctcgcgcc
     1561 cggctatgtc caggccaacc tcgcggtgct gccgaaggaa caggcctacg acttcctgct
     1621 cttctgccag cgcaacccgc ggccgtgccc gctcatcgag gtcaccgacg tgggctcgcc
     1681 ggagccggtc ggggtggcgc cgggcgccga cctccggacc gacctgccgc gctaccgcat
     1741 ctacaaggac ggcgtgctgg cggacgaggt gaccgacgtc cggcccttct ggcgcgacga
     1801 cctggtggcc ttcctgctcg gctgctcgtt caccttcgag tgggcgctgc tcgacgcggg
     1861 catcccgatg cgccacatcg agcagggaaa gaacgtggcg atgtggaaga cctcggtcca
     1921 gtgccggccc gccggccagt tccacggccc gatggtggtc tcgatgcgtc cgatccccgc
     1981 cgggctcctc tccaaggcgg tgaccgcgtc cgcgcgcttc ccgggcgccc acggggcgcc
     2041 ggtgcacatg ggcgatccgg ccgcgctcgg catcgccgac gtgaacaccc cggactgggg
     2101 cgactcggtg ggcgtgcgcg ccggcgaggt gccggtgttc tgggcgtgcg gggtcacccc
     2161 gcaggcggtg gcgctggcct cgaagcccgc gttcatgatc acccacagcc ccggccacat
     2221 gttcatcacc gacctgccga accacgcgct ggccgcgatt tgacgcttcg ctcgcggtga
     2281 cgatctggcc cgcgccgcgc ctcctgccgt gcggggaccg cgcggtctcc gtcgagctgg
     2341 gcgatgaggt caaccgggag ctgaacgccc gcgtcctcgc gctggagtac ctgctccagc
     2401 agcggcgcct gcccggggtg ggcgagaccg tccccagcta tcgcgcgctc ctcgtctact
     2461 acgacccgct ggtggtcgac tacgacacgc tcggggtgac gctccgcgag ctctgcgagc
     2521 gggcccggcc cgaggtgctg ccgcccgcgc gcacggtgga gctgccgtgc tgctacgagg
     2581 gcgagctggg cttcgagctc gaggcggcgg ccgagaagct cggcctcgtc cccgccgagg
     2641 tggtccggct gcacgcggcg gccgactact acgtgtactt cgtgggattc actcccggcc
     2701 tgccctacat gaccgggatg cccgaccggc tgaccatccc gcgcctgcag aacccgcgga
     2761 ccaagacacc gcccggcagc gtcagcatcg gcggcgtcca gtgctgcatc tactccgtcg
     2821 agagcccggg cggcttctgg gtcctgggcc gcaccccggt gcgcctctac gacccggacg
     2881 ctcccgatcc gatcctgctg cgcgcgggcg accacgtgcg cttccacgcg atcgaccgcg
     2941 ccgagtacga cgcgatcgcg gcccgggtcg agacgggccg ctacgcgccc cggatcaccg
     3001 gcgcggcctc atgagcgacc tgctcgtcca ggagccgggc ccgctcacca ccatccagga
     3061 cctcggccgc ttcgggcacc tgcgggtcgg catcccgacc tccggcccga tggatcgcga
     3121 cgcgttcgtg ctcgccaacc ggctcgtcgg caacccggac ggcgccgccg cgctcgagtg
     3181 caccttgatc ggcgcccgcg tcgagctgac cgacgaccgc ctggtcgcgg tgaccggggc
     3241 cgacatgtcg ccgaccgtga acggcgcgac ggtgccggcc tggcaggcgg tggcggtgaa
     3301 ggcgggcgac gtgctgcggc tcgggccgtc ccggggcggg gtgcgcgcct acctggcggt
     3361 ggcgggc
//
LOCUS       DATMCV010001118         2456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_263152, whole genome shotgun sequence.
ACCESSION   DATMCV010001118 DATMCV010000000
VERSION     DATMCV010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263152"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..661)
                     /locus_tag="VNU03_25565"
     CDS             complement(107..661)
                     /locus_tag="VNU03_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HWP77916.1"
                     /translation="MAQNATIFKVTLEIADIDRAYYHDHTLTIARHPSETDERMMVRL
                     LAFARHADEALAFGKGLGTDDEPDLWRKDLTGAIDVWIDVGQPDERRIRRACGRADAV
                     FVYSYGGRGAGLWWDDAGAKLDRVANLTVVNLPFAATQALAKLAQRNMRLHCTLQDGQ
                     MWVADSERSVAIEPQLLKGPPAAG"
     gene            763..1860
                     /locus_tag="VNU03_25570"
     CDS             763..1860
                     /locus_tag="VNU03_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HWP77917.1"
                     /translation="MEIKAAVCRATHVPVGIESIDIDRPLGREVLVRTVATGVCHSDL
                     HVVDGDGRFALDRGIVLGHEGAGIVEAVGDQVTGIRPGDHVVACLSGFCGSCAQCLSG
                     HPNVCVGGAVARGDSAAPRLSKQGQGIPSFAGIGSYAERMLLHENSVVKIDDDLPLDR
                     AALVGCGVLTGVGAALRSAGLEAGQTVAVFGCGGVGLSIVQGARIGGARQIIALDRFD
                     SKLEMARRVGATHVVNTSNDDPVQAVRALTGRAGVDHAFEAVGNTTLVRQAIESLAVR
                     GTATIVGVLPPDAKIEFPWMAIRPECRVQTSRMGGNRFRIDIPRYLDFYRQGRLLLDE
                     MVTRRGRLDDLNEAFRAMKAGEVARTVLMFD"
     gene            complement(1895..>2456)
                     /locus_tag="VNU03_25575"
     CDS             complement(1895..>2456)
                     /locus_tag="VNU03_25575"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWP77918.1"
                     /translation="GVLFADLVGFTTMAEEMTPEEVMALLRDFHGRMEEEVFRHGGCL
                     EKFIGDALLATFGVPDTGRRDATDALACARGMLAALTAWNLEREAAGRPPLQIGLGLH
                     YGLVVAGDIGSRRSMAFATVGDTTNVTSRLQALTRELRVRIVASGALVAAVEREAAEP
                     ALLRGLTTRGPTVLRGRDTPIDLWAE"
BASE COUNT          333 a          852 c          909 g          362 t
ORIGIN      
        1 ggggacgagc cggatgtact gaaagtaata gggcaggcac tcgtcgtcgt ccggacgtcc
       61 gggaccgcgg tcggtatcgg gcacaggata tctcctcggt gaggggctag ccggccgccg
      121 gcggaccttt cagcagctgg ggctcgatcg ccacgcttcg ctccgagtcc gccacccaca
      181 tctggccgtc ctgcagcgtg cagtgcagtc gcatgttccg ctgcgccagc ttcgcgagcg
      241 cctgggtggc cgcgaagggc aggttgacga cggtcaggtt ggcgacccgg tcgagcttgg
      301 cgccggcgtc gtcccaccag agcccggcgc cacgccctcc gtagctgtag acgaagaccg
      361 catcggctct gccgcaggct cgccggatcc gtctttcgtc cggctgcccc acgtcgatcc
      421 acacgtcgat ggccccggtc aggtccttcc gccagagatc gggctcgtcg tcggtcccca
      481 ggcccttgcc gaaggcgagc gcctcgtctg cgtgccgggc gaaggcgagg aggcgcacca
      541 tcatgcgctc atcggtctcc gatgggtgcc gcgcgatcgt cagggtatgg tcgtggtagt
      601 aggcgcggtc gatgtccgcg atctcgagcg tgaccttgaa gatggtcgcg ttctgggcca
      661 tgtccgacga cattgtggca cgagcgggcc ccggccgggg ccgcggaggc ccaccgtcgc
      721 gctatactcc ggcggacgca ggacacgcga ggaggcgttc ccatggagat caaggctgcg
      781 gtctgccgcg cgacgcacgt gccggtgggc atcgaatcca tcgacatcga ccggccgctc
      841 ggtcgcgagg tcctggtgcg gaccgtcgcg acgggggtct gccacagcga cctgcacgtg
      901 gtggacggag acgggcggtt cgccctcgac cgcggcatcg tgctcggcca cgagggagcc
      961 ggcatcgtcg aggcggtggg cgatcaggtg accgggatcc gtcccggcga ccacgtggtg
     1021 gcgtgcctct ccggcttctg cgggagctgc gcccagtgcc tgagcgggca ccccaacgtc
     1081 tgcgtcgggg gggcggtggc gcgcggcgac tcggcggcgc cgcggctgtc gaagcagggg
     1141 caggggatcc cgtcgttcgc gggcatcggc agctatgccg agcggatgct gctccacgag
     1201 aactcggtgg tgaagatcga cgacgacctg ccgctggatc gggccgccct cgtaggctgc
     1261 ggtgtcctca ccggggtcgg cgcggcgctc cggagcgcgg ggctcgaggc cgggcagacg
     1321 gtggcggtct tcggctgtgg cggcgtgggg ctgtcgatcg tgcagggagc gcgcatcggc
     1381 ggcgcccggc agatcatcgc gctcgaccgg ttcgacagca agctcgagat ggcgcggcgg
     1441 gtcggcgcga cccacgtcgt caacacgtcg aacgacgacc ccgtccaggc cgtgcgggcc
     1501 ctgaccgggc gcgccggcgt cgaccacgcg ttcgaggcgg tcggcaacac gaccctggtg
     1561 cgccaggcca tcgagagcct ggccgtccgc ggcaccgcca ccatcgtcgg cgtcctgccg
     1621 cccgacgcga agatcgagtt cccgtggatg gcgatccgtc ccgagtgccg cgtgcagacg
     1681 tcccgcatgg gcggcaaccg cttccgcatc gacatcccgc gatacctcga cttctaccgt
     1741 caggggcggc ttctgctcga cgagatggtc acccgccgcg ggcgcctcga cgacctcaac
     1801 gaggccttcc gcgcaatgaa ggcgggcgag gtcgcgcgca ccgtcctgat gttcgactga
     1861 gcgagcgggc gactgagcgg gcggggccgg ccggctactc cgcccacaga tcgatcggcg
     1921 tgtcgcgtcc ccgcagcacg gtggggcccc gcgtcgtcag acctcgcagg agcgcgggct
     1981 cggcggcctc gcgctcgacc gcggccacca gcgcgccgct ggccacgatg cggacccgca
     2041 gctcccgggt gagggcctgt agccggctgg tgacgttggt ggtgtcgccc accgtcgcga
     2101 aggccatgct gcgccggctg ccgatgtcgc cggccaccac caggccgtag tgcagaccga
     2161 gcccgatctg caggggcggc cgcccggccg cctcccgctc gaggttccag gcggtgaggg
     2221 ccgccagcat gccgcgggcg caggccagcg catcggtcgc gtcacggcgg ccggtgtcgg
     2281 ggacgccgaa ggtggccagg agcgcgtcgc cgatgaactt ctccaggcag ccgccgtgtc
     2341 ggaacacttc ttcctccatg cggccgtgga agtctcgcag gagcgccatg acctcctccg
     2401 gggtcatctc ctcggccatg gtggtgaagc ccaccaggtc ggcgaagagc acgccg
//
LOCUS       DATMCV010001119        10672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_132716, whole genome shotgun sequence.
ACCESSION   DATMCV010001119 DATMCV010000000
VERSION     DATMCV010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132716"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VNU03_25580"
     CDS             <1..1419
                     /locus_tag="VNU03_25580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWP77919.1"
                     /translation="SSARRGTIDVDAGSAHTTFNGRVEMQASPDLSVFVRGNYYEESR
                     DNGTPLQFNNTAAGSFATGVRAKTGDGSEWTGTVFTDFQSFHSTFSTQAADRNSETLA
                     LDQRVPTTAVGAALTWSRAFGAHRVLAGTDFRWIEGETDERVYVAGAFSRTRVAGGQQ
                     QLYGLFLQDVYTPIPPLEIVGGIRGDYWRAYDAFRRDTPPPAGIPARQTFSDIDRMGA
                     SPRLALLYHATPSTDLRASTYQGFRVPTINELYRVFRVRNDVTVANEHLRPERLTGGE
                     LGVAQRVGPFEARATAFWNDVRDLVINVTQVNRLPDCPVGTICRQRQNVDLARIRGIE
                     TELELRPARDWRIIGSYTYTDTEVIDSPQQRSLEGKRLAQVARHGAALTVRYDNPALF
                     TAAVTARYVGKQFEDDINTLMLGSYVLVDLFVSRRVTKWAEVFFAVENLLDTTYTTGR
                     TSDGVVSIGAPFMAHGGVRLQF"
     gene            1419..1829
                     /locus_tag="VNU03_25585"
     CDS             1419..1829
                     /locus_tag="VNU03_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HWP77920.1"
                     /translation="MGVVGIRVGPLAFAARWEETRAPRTCAAFRRLLPYRRRIIQARW
                     SGEAGWIPLGDFDLGVGPENPTSEPAPGALLFYPGGISETEILFPYGTARFASVKGPL
                     AGNHFLTIVEGRERLGEVGRLLCWEGAQDISFEA"
     gene            complement(1813..2724)
                     /locus_tag="VNU03_25590"
     CDS             complement(1813..2724)
                     /locus_tag="VNU03_25590"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWP77921.1"
                     /translation="MLAAGPAWADFEDGRRAYDQADYPAAYREWLPLAERGDPSAQFM
                     VGFMHERGQSVPADPAAAVTWYRRAAQQGDVHAQFRLGFLYAYGRGVDRDDVEAAVWY
                     GRAAEQGNQAARAALEQLRSEGRLAGPTGSREVLVLRAAAERGAAAAQYQLALRYAAG
                     EGVPRDPAEAVHWYREAAQQGLAPAQYQLGLRYANGDGVTRDFEEAVKWYQRAAAQGV
                     AFAQFNLGVRYANGQGVERDPVKAYQWFSLAAQGLLGREADTARQARDSIRGQMSPEQ
                     VAEGDALVKAWRAKPGRPGAAVATPRS"
     gene            complement(2774..3055)
                     /locus_tag="VNU03_25595"
     CDS             complement(2774..3055)
                     /locus_tag="VNU03_25595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77922.1"
                     /translation="MLSDRYVKGALTVIALALVAIAGNLWLTELRPRAAVAETPARYE
                     IGVPKAWGKLVGFSNNNLLLESSDGLLRIVDVEGREPEYPKIKVQINRR"
     gene            complement(3119..3991)
                     /locus_tag="VNU03_25600"
     CDS             complement(3119..3991)
                     /locus_tag="VNU03_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWP77923.1"
                     /translation="MAAMTYDRREQDLGNILLLEHVNVRVPDQQAATLFYIAGLGLTR
                     DPYLMVSVENMWANAGQTQFHLPTGKPNVVQGHVELIVPDLDALTRRLASVRQALAGT
                     QFAYEVRDKHVAVTCPWGNQFRCVGPGPEWADITLGIGRVEVPVARGAAAGIARFYQQ
                     ALRATTSVTQDDQGAAARVSVGYHQELVFRETDATFAPFDGHHIAIYIADFSGPHDWL
                     VQRSLVTEESNPYQYRFQVIVDPDGGQQRSELEHEVRCATHPMYMRPLVNRNAAQRQA
                     TYVRGRDAFVPGLL"
     gene            complement(4027..4245)
                     /locus_tag="VNU03_25605"
     CDS             complement(4027..4245)
                     /locus_tag="VNU03_25605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77924.1"
                     /translation="MDFGDYVTEKMAAARLGELREQGARLALLDQLGRGRRGLIPRLG
                     IGLTRLGRWLARDGGPARNGRVRAARVR"
     gene            complement(4262..4666)
                     /locus_tag="VNU03_25610"
     CDS             complement(4262..4666)
                     /locus_tag="VNU03_25610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77925.1"
                     /translation="MSLETETTIMIALIPFLAIWGLLRFVRAVERRRDARVARQIVLT
                     DAIHRELGAVAAPNVRRTWSGEWVVSMRLALAREGTVGTITRITNDLFRRLDRQDPPR
                     LRVVLLPQEPRPARRILATDSPRSAGRLSRAA"
     gene            complement(4752..5711)
                     /locus_tag="VNU03_25615"
     CDS             complement(4752..5711)
                     /locus_tag="VNU03_25615"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWP77926.1"
                     /translation="MSTMPTSCREIEPHLLASAMGEATRDSVELVRTHVAGCPECREE
                     LTRYRAVDGMVDSLRRAPGPDDDATLARAQLTSRLADLRSRMVTFGIFPSPLGPLLIG
                     RSEMGVALVEYLPASGSLTARVRRLLGDDAMEDRAATEGLRAELIEYLEGRRARLAWP
                     LDPRRMRSDFQRRVLEATAALPYGAVTSYAGIAARIGAPSAFRSVAQALRWNPLPIVI
                     PCHRVIGSGGDLVGYAGKRVELKQRLLAVEGVNVGPHDFKVHREAMYTLMHGEVEYCL
                     PTCGSLAERPLSDLTLFGTRERAEAAGFGPCSSCRPDLHPLPA"
     gene            complement(5708..6235)
                     /locus_tag="VNU03_25620"
     CDS             complement(5708..6235)
                     /locus_tag="VNU03_25620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWP77927.1"
                     /translation="MAEDAFEAVVGAHHAEIRRYLLRVTARASDADDLSQETFLRAFR
                     AYRALPSGANVRAWLFTIATNLGRNHFRAQSRRRRAYAAVALEAPAGAPDRTDGRVLA
                     DEARVRVEQVIGGLPEKQRLAFIMRKMHELDYAEIGTSLCCSEESARAHVFQALRKIR
                     KGLDSQLSAGTEPRS"
     gene            complement(6296..7294)
                     /locus_tag="VNU03_25625"
     CDS             complement(6296..7294)
                     /locus_tag="VNU03_25625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77928.1"
                     /translation="MKGRRLAGLRGSHVIFLLSTWAVVSFLALLVVGLAWTVKLKPQA
                     LADARAQRQEIVRVLARLADAQVRDKLDALVPVAAHLAQSWASHEPRALQRELDMMRA
                     MLQMPTLFVVSPDGRPLAFSPATGADGSSNLDLRYADRPVFQEAVRTQGPAVSGIVFG
                     RATRDFIVGAAAPIRIEQRIAAYLLGSIRLQASIEAIEQAAAGPGGWLVLIDGEKQAI
                     FLDPRTHQVTQQDWKGHPVVEELGRRRSDGLVAIDGGDEWLVTQALMPTLKVNLVFAA
                     SVRHILEGQRAVLLTLGATLLVSLGTSLALSTVVAIRFLREQQGAAPVESGVVARS"
     gene            7579..7836
                     /locus_tag="VNU03_25630"
                     /pseudo
     CDS             7579..7836
                     /locus_tag="VNU03_25630"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            7931..8542
                     /locus_tag="VNU03_25635"
     CDS             7931..8542
                     /locus_tag="VNU03_25635"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWP77929.1"
                     /translation="MGCCVPTGVGAVTRCAEVEAGASVLVIGCGGVGLNVVQGAKLAG
                     AGIIAAADLLDSKLTYAKDFGATHTINAKSDNVVDRVRELTQGRGVDYAFDAIGGEAT
                     TLQIVEAIRPGGTAVIVGMAALNVRAPITPYNMALQEKTIKGTMYGSVRPNVDFPKLV
                     DLYLDGRLKIDELVSRTYSLDQINEGFTALRSGQVARGVVVFN"
     gene            8569..9459
                     /locus_tag="VNU03_25640"
     CDS             8569..9459
                     /locus_tag="VNU03_25640"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWP77930.1"
                     /translation="MLRSVGRLLTRRPTGPLAPAAPLEAVLAGDDAPFLLARLLCGRD
                     RAAREAALPRYFQHRGIAFSRHRFTRPEGSGETYAVDLGEGARTLVLCAHHDAVPGSP
                     GANDNAAAVGILLHLIPRLPPRIPRGWRVRLLFTAAEEIDYLGARAYVEETPLDGIAG
                     VLSLELCGIGDAVALWDAEEQTPFLDAVTAALESAGLVRGVGHHVVGRIPLFGSDHRA
                     FAAAGVPAYGFTMVPVAHAESLRRFVLSPVRSAFRSLIRRPPPFHTYHTSGDTLETLE
                     PAALHRAAAALTAIVAGLPT"
     gene            complement(9466..10152)
                     /locus_tag="VNU03_25645"
     CDS             complement(9466..10152)
                     /locus_tag="VNU03_25645"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWP77931.1"
                     /translation="MGAMQDVARVAGALGEPSRLAILAALLEGEATVSDLATRVGLAQ
                     PRVSTHLARLRAAGLVTRVAMGRHRAYRADAARVKPLLDALYATGPAAVLRPSRQAGR
                     ERRGDTPLRRARTCYDHLAGVAGVELLEAVRKRGWLLPGAKSKPAYELSTGGTRALAR
                     RGVDVAAASESRRRFATACLDWTERRPHLGGALGARILDALCGKGYVRRRSKGRDVQI
                     VKPLAGWLAG"
     gene            10233..>10672
                     /locus_tag="VNU03_25650"
     CDS             10233..>10672
                     /locus_tag="VNU03_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77932.1"
                     /translation="MTRPIGPSGGRASGPPADPIAAVTHPDPYPYYRDLVARTPLYRD
                     EARGLWVAASADAVTAVLTSPLGRVRPAAEPVPTALVASPAADVFGRLARMTDGPGHA
                     TMKQAVSATLDSVDAPRALAHADRWARRLSAEIDPRSSPARVDE"
BASE COUNT         1482 a         3964 c         3756 g         1470 t
ORIGIN      
        1 agctcggcgc gccgcgggac catcgacgtg gacgcggggt cggcgcacac caccttcaac
       61 gggcgcgtcg agatgcaggc ctcgcccgat ctctcggtgt tcgtgcgcgg caactactac
      121 gaggagagcc gcgacaacgg gacgcccctg caattcaaca acaccgccgc gggctcgttc
      181 gcgacgggcg tgcgggcgaa gaccggagac ggcagcgagt ggaccggcac cgtcttcacc
      241 gacttccaga gcttccacag cacgttctcg acccaggccg ccgaccgcaa ctcggagacc
      301 ctcgctctcg accagcgggt gcccacgacg gcggtgggcg ccgcgctcac ctggagtcgc
      361 gccttcgggg cccaccgggt cctggccggc accgacttcc gctggatcga gggagagacc
      421 gacgagcggg tctacgtcgc gggagccttc tcgcgcacgc gggtggcggg cggccagcag
      481 cagctctacg gcctcttcct ccaggacgtc tacaccccga tcccgccgct cgagatcgtg
      541 ggcggcatcc gcggcgacta ctggcgcgcc tacgacgcgt tccggcggga taccccgccg
      601 cccgcgggca tccccgcgcg tcagaccttc tccgacatcg accgcatggg cgcgagcccg
      661 cggctggcgc tgctctatca cgcgacgccc tccaccgacc tgcgcgcgtc cacctaccag
      721 ggcttccggg tcccgaccat caacgagctg taccgggtgt tccgcgtgcg gaacgacgtc
      781 accgtcgcca acgagcatct ccgccccgag cggctcaccg gcggcgagct cggggtggcg
      841 cagcgggtgg gcccgttcga ggcgcgggcc accgcgttct ggaacgacgt gcgggacctg
      901 gtcatcaacg tgacgcaggt caatcggctg cccgactgcc cggtaggcac catctgccgt
      961 cagcggcaga acgtggacct cgcccgcatc cgcggcatcg agaccgagct cgagctgcga
     1021 ccggcgcgcg actggcggat catcggcagc tacacctaca ccgacaccga ggtcatcgac
     1081 tctccgcagc agcgcagcct cgagggcaag cgcctggccc aggtggcgcg gcacggcgcc
     1141 gccctcacgg tgcgctacga caatcccgcg ctgttcaccg ccgcggtcac cgcgcgctac
     1201 gtggggaagc agttcgagga cgacatcaac accctgatgc tcggcagcta cgtgctggtc
     1261 gacctcttcg tctcgcggcg cgtgaccaag tgggccgagg tcttcttcgc ggtcgagaat
     1321 ttgctcgaca ccacgtacac caccgggcgc accagcgacg gcgtcgtctc catcggggcc
     1381 ccgttcatgg cccacggcgg cgtgcgcctg cagttctgat gggcgtggtc gggatccggg
     1441 tgggaccgct cgccttcgcg gcgcgctggg aggagacgag ggcgccccgc acctgcgccg
     1501 cgttccggcg gctcctgccc tatcggcggc gcatcatcca ggcgcgctgg agcggcgagg
     1561 cgggctggat cccgctcggc gacttcgacc tgggagtggg acccgagaac cccacgagcg
     1621 agccggcccc gggcgcgctg ctcttttatc caggtgggat cagcgagacc gagatcctct
     1681 tcccctacgg gaccgcgcgc ttcgccagcg tgaaggggcc gctggccggc aaccacttcc
     1741 tcacgatcgt ggagggccgc gagcggctcg gcgaggtggg tcggctgctc tgctgggaag
     1801 gcgcgcagga catcagcttc gaggcgtagc caccgccgcg cccggacgcc ccggcttggc
     1861 gcgccacgcc ttcaccagcg cgtcgccctc cgccacctgc tccgggctca tctggccgcg
     1921 gatcgagtcc cgggcctggc gggcggtgtc ggcctcccgg ccgagcagcc cctgggcggc
     1981 gagcgagaac cactgatacg ctttcaccgg gtcccgctcg acgccctgac cgttggcgta
     2041 tcgcacgccg aggttgaact gggcgaaggc caccccctgg gcggcggcgc gctggtacca
     2101 cttcaccgcc tcctcgaaat cccgcgtcac tccgtccccg ttggcgtagc ggagaccgag
     2161 ctggtactgc gcgggggcca ggccctgctg tgcggcctcg cgataccagt gcaccgcctc
     2221 ggcgggatcc cggggcacgc cctcgccggc ggcgtagcgg agggcgagct gatactgggc
     2281 ggcggcggcg ccccgctcgg cggccgcgcg caggaccagg acctcgcgcg agccggtggg
     2341 cccggccagc cggccctcgg accggagctg ctccagggcg gcgcgggccg cctggttgcc
     2401 ctgctcggcg gcgcggccgt accacaccgc ggcttccaca tcgtcgcggt cgaccccgcg
     2461 cccgtacgcg tagaggaatc ccaggcggaa ctgggcgtgg acgtcgccct gctgagccgc
     2521 gcgccggtac caggtgaccg cggccgcggg atcggcgggc acgctctgcc cgcgctcgtg
     2581 catgaagccc accatgaact gcgcggaggg gtcgccgcgc tcggcgagcg ggagccactc
     2641 gcggtaggcg gcgggatagt cggcctggtc gtacgcgcgc cgcccgtcct cgaagtcggc
     2701 ccacgcgggg ccggcggcca gcaaggtccc gagcgcgacg cgcgccagcc gcgacgcgaa
     2761 gcgggcgatc aggctatcgc cggttgatct gcaccttgat ctttggatac tccggctccc
     2821 gaccttccac gtccacgatg cggaggagcc cgtcgctgct ctcgagcagg aggttgttgt
     2881 tggagaagcc gacgagcttg ccccacgcct tcggcacccc gatctcgtac ctggccgggg
     2941 tctcggccac cgcggcccgc ggccgtagct cggtgagcca gaggttgccc gcgatggcca
     3001 cgagggcgag cgcgatgacg gtgagcgcac ctttcacgta gcggtccgag agcatgcgca
     3061 acctcctaga gctcgggcta gagctcgggc tagatctggg gctcgggctt gggcctggtt
     3121 agagcaggcc gggcacgaag gcgtcgcgcc cgcgcacgta cgtcgcctgg cgctgggccg
     3181 cattccggtt caccagcggg cgcatgtaca tcggatgcgt cgcgcagcgc acctcgtgct
     3241 ccagctcgga tcgttgctgg ccaccgtccg ggtccacgat cacctggaag cgatactggt
     3301 acgggttgct ctcctcggtg acgaggctcc gctgcaccag ccagtcgtgc gggccggaga
     3361 agtcggcgat gtagatcgcg atgtggtggc cgtcgaacgg cgcgaacgtg gcgtcggtct
     3421 cgcggaacac cagctcctga tggtagccga cggatacccg cgcggccgcg ccctggtcgt
     3481 cctgggtcac gctcgtggtc gcgcgcagcg cctgctggta gaagcgggcg atgccggcgg
     3541 ccgcgccgcg cgccaccggg acctcgacgc gcccgatgcc gagcgtgatg tcggcccatt
     3601 cggggccggg gcccacgcag cggaactggt tgccccacgg gcacgtcacc gccacgtgct
     3661 tgtcccgcac ctcgtacgcg aactgggtgc ccgcgagggc ctggcgcacc gaggcgagcc
     3721 gccgcgtgag cgcgtcgagg tccgggacga tcagctccac gtgcccctgc accacgttgg
     3781 gcttgccggt gggcaggtgg aactgggtct gccccgcgtt ggcccacatg ttctccacgc
     3841 tcaccatcag gtaggggtcg cgcgtcagcc cgagccccgc gatgtagaaa agcgtggccg
     3901 cctgctggtc cggcacgcgc acgttcacgt gctccagcag aaggatgtta ccgaggtctt
     3961 gctcccgccg atcgtaggtc atcgccgcca tggcccgggc ccctcctctg gcctgcggca
     4021 agcatactac ctcacccggg cggcgcgcac ccgcccattc cgggccgggc ccccgtcgcg
     4081 ggcgagccag cggccgagcc tcgtcaggcc gatcccgagg cgcgggatca ggccgcgccg
     4141 gccccgcccg agctggtcga gcagggccag gcgggcaccc tgctcgcgga gctcgccgag
     4201 gcgggccgcc gccatcttct ccgtgacgta gtcgccgaag tccatggcca tccccctttc
     4261 gctaggcggc ccggctgagg cgaccggcgg accggggcga atcggtggcc aggatccgcc
     4321 gggccggccg gggctcctgc gggagcagca ccacgcggag gcggggcgga tcctgccggt
     4381 cgagccgccg gaagaggtcg ttggtgatcc gggtgatggt gccgacggtg ccctcgcgcg
     4441 cgagcgcgag ccgcatcgag accacccact cccccgacca ggtccgtcgc acgttcgggg
     4501 ccgcgaccgc gccgagctcg cgatggatcg cgtcggtcag cacgatctgc cgggccacgc
     4561 gagcgtcccg ccgccgctcg acggcgcgca cgaatctcag caggccccag atcgccagga
     4621 agggaatcag cgcgatcatg atcgtcgtct ccgtttctag gctcatcggt cgtctcctct
     4681 acccgtcgtt ccttcaccgg ttgagacacc gggatgggcg cggtgtgaaa cgacggagag
     4741 gacgcgtgcg gctaggcggg gagcggatgc agatcgggcc ggcagctcga gcaggggccg
     4801 aagccggcag cctcggcgcg ctcgcgcgtg ccgaacagcg tgagatcgga gagcgggcgc
     4861 tcggcgagcg acccacaggt cggcaggcag tactccacct cgccgtgcat cagggtgtac
     4921 atcgcctccc ggtgcacctt gaaatcgtgg ggtccgacgt tcacgccctc caccgcgagc
     4981 agccgctgct tcagctccac gcgcttgccc gcgtagccga cgagatcccc gccgctgccg
     5041 atcacccggt ggcacgggat cacgatcggc agcgggttcc agcgcagggc ctgggcgacc
     5101 gaccggaatg ccgacggcgc gccgatgcgg gccgcgatgc ccgcgtagga ggtgaccgcg
     5161 ccgtagggca gcgcggcggt cgcctcgagc acgcggcgct ggaagtcgct ccgcatccgc
     5221 cgggggtcga gcggccacgc gagccgcgcg cgccggccct cgaggtactc gatcaattcg
     5281 gcccgcagcc cctcggtcgc cgcgcgatcc tccatcgcgt cgtcgccgag cagccggcgc
     5341 acgcgcgcgg tgagcgagcc ggaggcgggc aggtactcca cgagggcgac ccccatctcg
     5401 gagcgaccga tgaggagcgg ccccaggggc gacgggaaga tgccgaaggt caccatgcgg
     5461 gagcggaggt cggccagccg cgaggtgagc tgggcccgcg cgagcgtcgc gtcgtcgtcc
     5521 ggccccggcg cacgccggag cgaatcgacc atgccgtcca ccgcgcgata ccgcgtgagc
     5581 tcctcgcggc actcggggca ccccgccacg tgcgtgcgca cgagctcgac cgagtcccgc
     5641 gtcgcctcgc ccatggccga ggccagcagg tgcggctcga tctcccggca gctcgtaggc
     5701 atcgtgctca tgatcgcggc tccgtcccgg ccgatagctg gctgtccagg cccttgcgga
     5761 tcttccgcag cgcctggaag acgtgggcgc gggcgctctc ttccgagcag cacaggctcg
     5821 ttccgatctc cgcgtagtcc agctcgtgca tcttccgcat gatgaaggcc agtcgctgct
     5881 tctcgggcag ccccccgatc acctgctcca ccctcacccg cgcctcgtcg gccagtaccc
     5941 gcccatcggt ccgatccggc gcccccgccg gggcctccag cgccaccgcc gcgtaggccc
     6001 gacgccgacg cgactgcgcc cggaaatggt tccggccgag gttggtcgcg atcgtgaaca
     6061 gccacgcccg cacgttcgcc ccagacggaa gggcgcggta cgcccggaac gcgcgaagga
     6121 aggtctcctg cgacaggtcg tcggcatcgc tggcgcgcgc cgtgacccgc agcaggtatc
     6181 gcctgatctc cgcgtgatgg gctcccacca ccgcctcgaa ggcatcctct gccatcaccc
     6241 tgcccattga gaccccggtg gccccgggct gtgaaaagag tccggtctcc accggctaac
     6301 tccgcgccac cacgccactc tcgaccgggg ccgcgccctg ctgctcccgc aggaaccgga
     6361 tcgccaccac cgtactgagc gccaggctcg tccccaggga gacgagcagg gtggctccca
     6421 gggtcaggag cacggcccgc tgaccttcca ggatgtggcg taccgaggcc gcgaagacca
     6481 ggttgacctt cagcgtcggc atcaaggcct gggtcaccag ccactcgtcg ccgccgtcga
     6541 tggccaccag gccgtcggaa cggcgccgac ccagctcctc gaccaccgga tggcccttcc
     6601 agtcctgctg ggtgacctgg tgggtccgcg ggtcgaggaa gatcgcctgc ttttccccgt
     6661 cgatcagcac gagccagccc cccggcccgg ccgcggcctg ctcgatcgct tcgatcgagg
     6721 cctggagccg gatgctcccg agtaggtagg ccgcgatccg ctgctcgatc cggatcggcg
     6781 ccgccgcgcc cacgatgaag tcgcgggtgg cccggccgaa cacgataccc gagacggccg
     6841 gcccctgggt gcgcaccgcc tcctggaaca ccggccgatc ggcgtaacgg aggtcgagat
     6901 tcgagctgcc gtccgccccc gtcgccgggc tgaatgccag gggccgaccg tcggggctca
     6961 ccacgaagag cgtgggcatc tgcaacatcg cgcgcatcat gtcgagctcc cgctgcaggg
     7021 cgcgcggctc gtgagaggcc cagctctggg ccaggtgggc cgccaccggg acgagcgcat
     7081 cgagcttgtc ccgcacctgc gcgtcggcca ggcgggccag cacgcgcacg atctcctggc
     7141 gctgggcgcg cgcgtccgcg agcgcctgcg gcttgagctt caccgtccac gcgagcccga
     7201 ccaccaggag ggcgaggaac gacaccaccg cccacgtgga gagcaggaag atcacgtgtg
     7261 atccgcgcag accggccagg cgccgtccct tcatcgaggg tccccgttca ttggggaccg
     7321 atgataccgc gcttgacagg gccccgcgcc ccgccgcatc atcggccgcg cggtgtccgc
     7381 ctcaccccga ccacccctca ccctgaccct ctcccccgag gggagagggg gagcatggag
     7441 agtgccgtga aagccgccgt cctctacaag gccaataccc cgctcgaggt cgtggagctc
     7501 acccaggaag gcccgaaggt gggggaggcc cgggtcaagg tcaaggccgc gggcatctgc
     7561 cacagcgact ggcacatcat gaacggggac tggacgccgc ccctgcccat ggtgctgggg
     7621 cacgaggcgg ctggcatcgt ggaggaggtg ggcgcgggcg tcaccaacgt gacgcccggc
     7681 gaccacgtca tcttctcgtt ccgaccgcag tgcgggcgct gcctctactg ctcgatgggc
     7741 cgctcgatcc tgtgcgacgg ccacgcctcg ccgcgctggg gcatgctcga cggcacctac
     7801 cggttgaagc gcggcgggca ggacatcttc cagatggcgc gcatcggaac cttctccgag
     7861 aaggtggtgt gcccggccga gatgctggtg cccatccgcc cggagatgcc gtggccccca
     7921 ggcggccctg atgggctgct gcgtacccac cggcgtcggc gcggtcaccc ggtgcgcgga
     7981 ggtggaggcg ggcgcgtccg tgctcgtcat cgggtgcggc ggcgtggggc tcaacgtggt
     8041 gcagggggcg aagctggccg gggccggcat catcgcggcg gccgacctgc tcgacagcaa
     8101 gctcacctac gccaaggact tcggcgcgac ccacaccatc aatgccaaga gcgacaacgt
     8161 ggtggaccgc gtgcgcgagc tgacgcaggg gcgcggggtc gactacgcct tcgacgccat
     8221 cggcggcgag gccacgacgc tgcagatcgt ggaggcgatc cggcccggcg gcaccgcggt
     8281 gatcgtgggc atggccgcgc tgaacgtgcg cgcaccgatc acgccctaca acatggcgct
     8341 gcaggagaag accatcaagg gcacgatgta cggctcggtt cgcccgaacg tggacttccc
     8401 gaagctggtg gatctctatc tcgacggccg gctcaagatc gacgagctgg tcagccgcac
     8461 ctactcgctc gatcagatca acgaaggatt caccgcgctg cggagcgggc aggtcgcccg
     8521 cggcgtggtc gtgttcaact gagcgcgcgc cacttcctgg gccggctggt gctgaggtcg
     8581 gtggggcgat tgctcacccg ccgccccact ggccctctcg cgcctgccgc gccgctcgag
     8641 gcggtcctgg ccggggacga cgcgcccttc ttgctggccc ggctgctgtg cggccgcgac
     8701 cgggcggcgc gcgaggcggc cctgccccgc tacttccagc atcgcggcat cgcgttctct
     8761 cggcatcgct tcacgcggcc ggagggctcg ggcgagacct acgcggtcga cctcggcgag
     8821 ggcgcgcgga ccctcgtgct gtgtgcgcac cacgacgcgg tgccgggcag tcccggcgcc
     8881 aacgacaacg cggccgcggt gggcatcctc ctgcacctga ttccccggct ccccccccgc
     8941 atcccgcgcg gctggcgggt gcgcctgctc ttcaccgcgg cggaggagat cgactacctg
     9001 ggcgcgcgcg cctacgtgga ggagaccccg ctcgacggga tcgcgggggt gctgagcctc
     9061 gagctgtgtg gcatcggaga cgcggtcgcc ctgtgggacg cggaggagca gactcccttc
     9121 ctcgatgcgg tcaccgccgc cctcgagtcg gccgggctgg tccgtggcgt gggccatcac
     9181 gtggtgggac gcatcccgct cttcggcagc gaccaccggg cgttcgcggc ggccggggtg
     9241 cccgcgtacg gcttcaccat ggtgccggtg gcccacgcgg agtcgctccg ccgcttcgtg
     9301 ctgagcccgg tacgctcggc cttccggagc ctgatccgcc ggccgccgcc gtttcacacc
     9361 taccacacga gcggcgacac gctcgagacc ctcgagcccg ccgcgctcca ccgggcggcc
     9421 gccgcgctca ccgcgatcgt cgcgggcctg cccacctagc tcgggctacc ccgcgagcca
     9481 ccccgcgagc ggcttgacga tctgcacgtc ccgccccttc gaccggcggc ggacgtagcc
     9541 cttcccgcac agcgcgtcga ggatgcgcgc gccgagcgcc ccgcccagat gcggccggcg
     9601 ctccgtccag tcgagacacg cggtggcgaa ccgacggcgc gattcgctcg ccgcggcgac
     9661 gtccaccccc cgccgcgcga gggcccgcgt gcctccggtg gagagctcgt aggccggctt
     9721 gctcttcgcg cccggaagca gccagccgcg cttgcggacc gcctcgagga gctccacccc
     9781 ggccacgccg gcgagatggt cgtagcaggt ccgcgcgcgc cggagcggag tgtctccccg
     9841 gcgctcgcgc ccggcctgcc gcgacggtcg gagcaccgcc gccggcccgg ttgcgtacag
     9901 cgcgtcgagc agcggcttca cccgggccgc gtccgcgcga taggcgcggt gccggcccat
     9961 ggcgacccgg gtgaccaggc cggccgcgcg cagacgcgcc agatgggtcg agacgcgggg
    10021 ctgggcgagc ccgacgcggg tcgccaggtc gctgaccgtc gcctcgccct ccagcagggc
    10081 ggccaggatc gcgagccgcg acggctctcc cagggcaccc gccacccgcg ccacgtcttg
    10141 catcgctccc atcgcgcggg tagtataccc ttcgttcgtg tatatatctc ctggttgaga
    10201 tatatggagg caggaaatat atggaggcac acatgaccag accgatcggc ccgagcggcg
    10261 gccgcgcgtc ggggccgccg gccgacccga tcgcggcggt cacgcacccg gatccctacc
    10321 cgtactaccg cgacctggtc gcccgcaccc cgctctaccg ggacgaggcc cgggggttgt
    10381 gggtggccgc gagcgcggac gcggtgactg cggtgctcac gagtccgctc gggcgggtgc
    10441 gccccgccgc ggagccggtg cccaccgccc tcgtcgcctc cccggcggcc gacgtcttcg
    10501 ggcggctcgc gcgcatgacc gacggtcccg gccacgcgac gatgaagcag gcggtgtcgg
    10561 ccacgctgga ctcggtcgat gctccccgtg ccctcgcgca cgccgaccgc tgggcccgac
    10621 gcctctcggc ggagatcgac ccgcgctcga gcccggcgcg ggtggacgag tt
//
LOCUS       DATMCV010001120         5542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_330498, whole genome shotgun sequence.
ACCESSION   DATMCV010001120 DATMCV010000000
VERSION     DATMCV010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330498"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VNU03_25655"
     CDS             complement(<1..1326)
                     /locus_tag="VNU03_25655"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HWP77933.1"
                     /translation="MGPLSVRYHFSGIAGVGMNPLAQLMRARGHAVQGSDRSFDQGKN
                     LDVAGRLTELGIVVKPQDGSAVSPNLDRFVYSTAVEAETPEVRAARSYGIERVPRPAL
                     LAEVVDAGRPGVAIAGTSGKSTITGMVGWLTREAGRSATVLGGAALAGEGGAGCFLPG
                     AADGPVAAEACESDGTLTGYHAALGLIHNISRDHGEVEALLPQFETFAKRCGLLLVNA
                     ASPEAAEVGRAVGARSYGIDPGADARLEVTSVGPHRASGVLHVPGGPLALDVPQPGRH
                     NLENAAAAALIALELGLGPATVTGLLARFPGVARRFEVIAVTAGGIRVVDDYAHNGEK
                     LRAAITTAQAGADRVLAVFQPHGFGPARFLRAELREMMPRLLRPQDRLCYAEIFYAGG
                     TVARDVSSRMLADDLPAAMDCGYAPDHRAVVDWLRAQARSGDTVLIMGAR"
     gene            1478..2200
                     /locus_tag="VNU03_25660"
     CDS             1478..2200
                     /locus_tag="VNU03_25660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77934.1"
                     /translation="MDTETVEARAVTVKGRFCSSPNTASGGYIAGLLGRLMDGPSRVS
                     LEQSVPVDRALGIERLSDGALALTDGVATLAHASPAALQLEPPVPVSYAEAEWASERY
                     LGFTEHSAPGCFVCGPAREWGDGLAIYPGPIPGRRLVAAPWAPDPTLFDKHGTVRPEF
                     VWAALDCPTGFALLEAFGRRKVTLSQLTASLIRPLPVGARWIVMGWPFAAEGRTLLGA
                     SAIFSESGELHALASAVLVCAD"
     gene            complement(2202..2660)
                     /locus_tag="VNU03_25665"
     CDS             complement(2202..2660)
                     /locus_tag="VNU03_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP77935.1"
                     /translation="MDDLYFEDFTPGREFRTEGVTVTESQILDFALAFDPQPFHLDTE
                     AAKGTIFGGLIASGFHTMALTFRLFAQTRALVACSLGSPGVDELRWLRPVRPGDTLRA
                     TVQVVEQRPSTSKPDRGIVRLHWTAVNQRSEPVLTMVSMQLLRRRAPAPA"
     gene            complement(2660..3598)
                     /locus_tag="VNU03_25670"
     CDS             complement(2660..3598)
                     /locus_tag="VNU03_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HWP77936.1"
                     /translation="MPRVITVGAAQLGPIARSESRSDVVKRLVSLLGQAHARGCDLVV
                     FPELALTTFFPRWWMEDQAEIDAFFEREMPGPETRPLWDEAARLGLAFCLGYAELCRE
                     GGALRRYNTAILVDRGGRLVGKYRKVHLPGHVQHEPWRPFQHLEKRYFDVGNLGFPVW
                     RALGGVLGMCICNDRRWPETYRVMGLRGVEMILLGYNTPVHNPPAPEHDLLGPFHNHL
                     VMQAGAYQNGTWVVGVAKCGTEEGCDMIGQSAIIAPSGEIVALASTLGDELVTARCDL
                     DLTRSFKETIFNFALHRRPEHYRLIVERAGAEPPPA"
     gene            complement(3688..4029)
                     /gene="trxA"
                     /locus_tag="VNU03_25675"
     CDS             complement(3688..4029)
                     /gene="trxA"
                     /locus_tag="VNU03_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWP77937.1"
                     /translation="MSNEAPWLLHLDEKTYDEALVAAEGVMMVDFWAEWCGPCRAVGP
                     ILEDLARTSGGTVTLAKVNVDENPGLAARYGIRSIPTILFVKDGKVQDQLIGAVPKPQ
                     IQAKLNAVSSK"
     gene            4253..5281
                     /locus_tag="VNU03_25680"
     CDS             4253..5281
                     /locus_tag="VNU03_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWP77938.1"
                     /translation="MNLGELGRIFSSDLAVDLGTSNTRLYVRGQGVVLDEPSVVAVDM
                     SGAVQAVGNEAKAMLGRAPGSVQVVRPLRHGVIADFDRTQKMFRFFISKTRRRWSLVQ
                     PRLVIVTPSGITQVERRAVRDAARQAGAREVYLIQASMAAALGAGLPVAEPGASMIVD
                     IGGGTTEVAVMSLCGVVYSESLRIAGDDMNQSIIQFLRRSYDLLIGERRAEDAKIKLG
                     SAYPKGDDNEGMEVQGRDLLDGLPKTVVVTALEVREAMREPVADIVTAVRACLEKTPP
                     ELAADISDNGIVLTGGGALLRGLDALLHRETGLSIRATEEPLTCVARGAGMVLDQLDI
                     LKRVAIPA"
BASE COUNT          789 a         1967 c         1984 g          802 t
ORIGIN      
        1 gcgcgcgccc atgatgagca cggtgtcccc ggagcgggcc tgggcgcgca accagtcgac
       61 gaccgcgcgg tgatcgggcg cgtagccaca gtccatcgcg gcgggaaggt cgtccgccag
      121 catgcggctc gacacgtcgc gggcgacggt gccgcccgcg tagaagatct cggcgtagca
      181 gagacggtcc tggggccgta gcaggcgcgg catcatctcg cgcagctcgg cgcgcaggaa
      241 gcgcgcgggc ccgaagccgt gcggctggaa gacggcgagc acccggtccg ccccggcctg
      301 cgcggtcgtg atggcagcac ggagcttctc cccgttgtgg gcgtagtcgt ccaccacgcg
      361 gataccgccc gcggtgaccg cgatcacctc gaagcggcgg gcgacccccg ggaagcgcgc
      421 gagcagtccg gtcacggtgg cgggcccgag gccgagctcc agcgcgatga gcgcggcggc
      481 ggccgcgttc tccaggttgt ggcgaccggg ctgcggaacg tccagcgcga gcgggccgcc
      541 cgggacgtgg agcaccccgg atgcgcgatg cggcccgacg ctcgtgacct cgaggcgcgc
      601 gtcggcgccg gggtcgattc cgtagctgcg cgcgcccacc gcgcggccca cctcggccgc
      661 ttccggcgag gccgcgttga cgagcagcag cccgcagcgc ttggcgaagg tctcgaactg
      721 gggcagcagc gcctccacct cgccgtgatc gcggctgatg ttgtggatga gcccgagcgc
      781 ggcgtgatag ccggtgagcg tgccgtcgga ctcgcaggcc tccgcggcca ctgggccgtc
      841 ggccgcgccg ggcaggaagc agcccgcccc gccctcgccg gcgagcgcgg cgccgcccag
      901 caccgtcgcc gatcggcccg cctcccgggt gagccagccc accatgccgg tgatggtgct
      961 cttgccgctc gtgcccgcga tggccacgcc ggggcgcccc gcgtccacca cctcggccag
     1021 cagcgcgggc cggggcacgc gctcgatgcc gtacgagcgc gccgcgcgca cctccggcgt
     1081 ctcggcctcc accgcggtgg agtagacgaa gcggtcgagg ttcgggctca ccgcggagcc
     1141 gtcctgcggc ttgaccacga tgccgagctc ggtgagccgg ccggccacgt cgaggttctt
     1201 gccctggtcg aaggatcgat ccgagccctg gaccgcgtga ccgcgggcgc gcatgagctg
     1261 ggcgagcggg ttcatcccga cccccgcgat gcccgagaag tggtagcgca cgctcaatgg
     1321 tcccacggat cggccgaatt atttcacgat tggaccggcg gatattcgcc cacctgccag
     1381 ggcgaaccgc ccgaaccggc aatggaagtg cttgatttgc cgagcaggtg tcgatggcac
     1441 cgggtatgcg ttcacgggcc caccaaggga ggacgccatg gacacggaga ccgtcgaggc
     1501 cagagccgtt accgtgaagg gccggttctg cagctccccg aacaccgcca gcggaggtta
     1561 catcgccggg ctgctgggcc ggctgatgga cggcccctcg cgggtgagcc tcgagcagtc
     1621 ggtcccggtg gatcgtgccc tcgggatcga gcgcctctcc gacggcgccc tggccctgac
     1681 ggatggcgtg gcgaccctgg cccacgcgag cccggccgct ctccagctcg agccgccggt
     1741 gccggtgtcc tacgcggagg ccgaatgggc ctccgagcgc tacctcgggt tcaccgagca
     1801 ctcggccccc ggctgcttcg tctgcggccc cgcccgcgag tggggtgatg ggctcgcgat
     1861 ctatcccggg cccatcccgg gacggcgtct cgtcgcggcg ccgtgggctc ccgatcccac
     1921 cctcttcgac aagcacggga ccgtgcgtcc cgagttcgtc tgggccgcgc tcgattgccc
     1981 gaccggcttc gcgctgctcg aggccttcgg ccgccgcaag gtcacgctga gccagctcac
     2041 cgcgagcttg atccgccccc tgccggtggg cgcgcgctgg atcgtgatgg gctggccgtt
     2101 cgcggcggag ggccgcacgc tgctgggcgc ctccgcgatc ttctccgaga gcggcgagct
     2161 gcacgctctc gcctcggcgg tgctggtctg cgccgactga gctaggccgg cgcgggcgcg
     2221 cggcgcctga ggagctgcat gctcaccatg gtgagcaccg gctcgctgcg ctggttcacc
     2281 gccgtccagt gcaggcgcac gatgccccgg tcgggcttcg aggtggacgg gcgctgctcc
     2341 acgacctgca cggtggcccg cagcgtgtcc ccgggccgca ccggccggag ccagcgcagc
     2401 tcgtccaccc ccggcgagcc caggctgcac gccaccagcg cgcgggtctg cgcgaagagc
     2461 cggaaggtca gcgccatggt gtggaacccg ctcgcgatga gccccccgaa gatcgtgccc
     2521 ttcgcggcct ccgtgtccag gtggaacggc tgcgggtcga aggcgagcgc gaagtcgagg
     2581 atctggctct cggtgacggt cacgccctcg gtgcggaact cccggccggg cgtgaaatcc
     2641 tcgaagtaga gatcgtccat caggcgggcg gcggctcggc gccggcgcgc tccacaatga
     2701 gccggtagtg ctccggtcgg cggtggagcg cgaagttgaa gatcgtctcc ttgaacgagc
     2761 gcgtgagatc gagatcgcac cgcgcggtca cgagctcgtc gccgagggtc gaggcgaggg
     2821 ccacgatctc gcccgaaggg gcgatgatcg cgctctggcc gatcatgtcg cagccctctt
     2881 ccgtgccgca cttggccacc ccgaccaccc acgtgccgtt ctggtaggct cccgcctgca
     2941 tcaccaggtg gttgtggaag ggcccgagca ggtcgtgctc gggcgcgggc ggattgtgca
     3001 ccggggtgtt gtagccgagc aggatcatct ccacgccccg cagccccatc acccggtacg
     3061 tctcgggcca ccggcgatcg ttacagatgc acatgccgag cacgccgccc agcgcccgcc
     3121 agaccggaaa gcccaggttg cccacgtcga agtagcgctt ctcgaggtgc tggaagggcc
     3181 gccacggctc gtgctgcacg tggccgggca ggtgcacctt gcggtacttg cccacgaggc
     3241 gaccgccgcg gtcgacgagg atcgcggtgt tgtaccgtcg cagcgcgccg ccctcgcggc
     3301 agagctcggc gtagcccagg cagaacgcca gaccgagtcg cgccgcctcg tcccagagcg
     3361 gccgggtctc cgggccgggc atctcgcgct cgaagaatgc gtcgatctcc gcctggtcct
     3421 ccatccacca gcgagggaag aaggtggtga gcgccagctc cgggaagacc acgaggtcgc
     3481 agccacgggc gtgggcctgg ccgagcaggc tcacgagccg cttgaccacg tcggaccggc
     3541 tctcgctgcg ggcgatcggg ccgagctggg cggcaccgac cgtgatcacg cggggcatgg
     3601 ggcggagcct agcacgggcg atcgggtgag gaaacggaag cgagcgggca gccggctcgg
     3661 cgcgaaggcc ggccggctgc ccggagacta cttgctggaa acggcgttca gcttggcctg
     3721 gatctgcggc ttcgggacgg ctccgatgag ctgatcctgg accttcccgt ccttcacgaa
     3781 caggatggtc gggatcgagc ggatgccgta ccgggcggcc aggcccgggt tttcgtccac
     3841 gttcaccttc gccagcgtca cggtgccacc cgaggtgcgg gccagatctt ccagaatggg
     3901 gccgacggcc cggcaggggc cgcaccactc cgcccagaag tccaccatca tcacgccttc
     3961 ggcggccacg agcgcctcgt cgtaggtttt ctcgtccaga tgcagcaacc agggggcttc
     4021 gttgctcatg tcctgtgtcc tcccgtgctt gagatgaggg aaccgaccgg ggcgatgcag
     4081 cggcatcata gcgccattga ccgccgagga gtcactgccg agatgagcgg caaggactga
     4141 caggcaccgt caccgctcgg ccggaatcat cctcgcaacc tattaatttt attggcgcca
     4201 atcgtggtcc gcttcttgaa gtcttgctgc gcggcgggag ggccacgagc acatgaatct
     4261 tggcgaactt gggcggatct tctcgagcga cctggcggta gatctgggca cgtccaacac
     4321 ccggctctac gtccggggac agggcgtcgt cctggacgag ccttccgtcg tcgcggtcga
     4381 catgagcggc gcggttcagg ccgtcggcaa cgaggccaag gccatgctcg gccgggcccc
     4441 gggctcggtc caggtcgtcc ggccgctccg ccacggcgtc atcgccgact tcgatcgcac
     4501 ccagaagatg ttccggttct tcatcagcaa gacccgccgc cgctggagcc tggtgcagcc
     4561 gcgcctggtc atcgtcacgc cctccggcat cacccaggtg gagcgccgcg cggtccggga
     4621 tgccgcccgg caggcggggg cccgcgaggt ctacctgatc caggcctcca tggccgccgc
     4681 cctcggcgcc ggcctgccgg tggccgagcc cggcgcgagc atgatcgtgg acatcggggg
     4741 tggcaccacc gaggtcgcgg tgatgtccct ctgcggggtg gtgtactcgg agtcgctgcg
     4801 catcgcgggc gacgacatga accagtcgat catccagttc ctgcgccgca gctacgacct
     4861 gctcatcggc gagcggcggg ccgaggacgc caagatcaag ctgggctcgg cctatcccaa
     4921 gggcgacgac aacgagggga tggaagtcca gggccgggac ctgctcgacg gcctgcccaa
     4981 gacggtggtc gtcaccgccc tcgaggtgcg cgaggcgatg cgcgagccgg tggcggacat
     5041 cgtgaccgcg gtgcgggcct gcctcgagaa gacaccgccc gagctggccg ccgacatcag
     5101 cgacaacggc atcgtcctca ccggcggcgg cgccctcctg cgcggcctcg acgccctgct
     5161 ccaccgggag acggggttgt cgatccgcgc caccgaggag ccgctcacct gcgtggcccg
     5221 gggagctggg atggtcctcg atcagctcga catcctgaag cgcgtcgcga tccccgctta
     5281 gccttccagg gcgacgggga gccggcgccc gccggcgcac gttgcctgcc ggcccctcga
     5341 gaggatacgc tcggggcatt ccatgtcact ccgcggagcc ttctgagccg acgggatgcc
     5401 gaacgatcgc tacggtctgt cgctcacgac gacgcccccc acgaccgccg tgtacgtcga
     5461 cgcggtggat cggctgctcg ccgcgcgcga gggggcgctc gagggattcg atcgcgcgct
     5521 cgccgccgat cccggcttcg cg
//
LOCUS       DATMCV010001121         7728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_60976, whole genome shotgun sequence.
ACCESSION   DATMCV010001121 DATMCV010000000
VERSION     DATMCV010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60976"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VNU03_25685"
     CDS             <1..221
                     /locus_tag="VNU03_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HWP77939.1"
                     /translation="PAGGLITYSANTPDLFRRSATYVDRIVKGAKPGDLPVEQATRFD
                     LVIDLKAARALGLTIPQSLLLRADQVIE"
     gene            441..1958
                     /locus_tag="VNU03_25690"
     CDS             441..1958
                     /locus_tag="VNU03_25690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77940.1"
                     /translation="MLVIWALAFGGALALWPAPPPARAADGQITLAVHISLAPTWFDP
                     AETAGVITPFLMLYALHDGLVKPMPGNAWTPSLAESWTQSKDGLTYEFVLRKGVRFHN
                     GDLLTAEDVKFSFERYKGAAAGTLKAHVAAIEIVDAHRVRFRLKQPWPDFMTFYATPA
                     SGAAWIVPKAYLERVGDDGFKKAPVGAGPYRFVSFSPGIELVLEAHDGYWRKAPAVKR
                     LVFKSIPDDSTRLAMLKRAEADVAYSLRGPLGEEVRRTPGLTLKVTYPTFTQWLTFTQ
                     QWDPKSPWADQRVRLAANLAIDRKTLSQAEYLGFGKAASSIIPREYEFYWAPPEYPHD
                     PARAKQLLAEAGYPKGFDAGEVATDVVFAPEAEAVINGLRSVGIRARLRPMERAAFYK
                     ADQDKQFKHLIRVGSGTAGNAATRIEAFVLSSGIRSYGGYPDIDALFRDQAAEPDRKR
                     REALLHRIQQLMHERAMFAPIVEPPLLTGIGPRLAEVPTIPGHPYLSPYEDLRLK"
     gene            complement(1951..3327)
                     /locus_tag="VNU03_25695"
     CDS             complement(1951..3327)
                     /locus_tag="VNU03_25695"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HWP77941.1"
                     /translation="MLEEPTSLRIDDPGVRALFTESARFQSWLDVEAALARAQAELGI
                     IPEAAAREIVRKAHLSFLDLAAVREGLARTGHPLVPLVWELDRACEGDAGGFVHWGAT
                     TQNVTQTGQLLQVRRAHEIFLRQLAAILTTLADLAERTRDVLLPGRTHGQHAVPATFG
                     FKVAVWIDELGRHVDRLQGCEGRVFVAMLGGGAGTLASLGELGLATQEKMAAHLGMVP
                     MRLPARTIGDHQAEYVTLLGMLASTCSKIGREVYTLMKQEFGEVEEPVPPGTVGSSTM
                     PQKRNPKLSQDIIAAAAQVRALVPLALEAMQTEHEADRTTSIMMSRALVQACELTGDL
                     LQRMMVLFDGLQVFPDRMRENLDLSGGLIMAEALMLELGRQIGRQRAHDAVYEAAQAS
                     VTESRPFREMLALDPHVSAGLTPSQMETLLDPARYTGLCREFAERGAALGREIAAAIE
                     RRPAGRVT"
     gene            complement(3343..3771)
                     /locus_tag="VNU03_25700"
     CDS             complement(3343..3771)
                     /locus_tag="VNU03_25700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77942.1"
                     /translation="MTRRPAALAAAAGLALALVALAWAALAPLPEGAHALIFVIPPGT
                     AARLKAGEAASVLPSPIHLTVGVRDVLVLTNDDDVIHQVGPIILGSRQTYRIPFRKPG
                     RFQYACSLHAAGTLALVIDPPPAAGLERLRWRLAQLTARS"
     gene            complement(3768..4211)
                     /locus_tag="VNU03_25705"
     CDS             complement(3768..4211)
                     /locus_tag="VNU03_25705"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HWP77943.1"
                     /translation="MRRLLVLALAGLVVAPAAVAQEATIRLEKPWVRRAPAMPDARPG
                     AESTAAGYVILLNRGPDRDALVAASADVAARVELHETRNMSGMMMMERVDKVELAPGA
                     RVELKPGSYHLMLIGLKRALTPGQTVTLILEFERGGRMTTRAEVR"
     gene            complement(4216..4827)
                     /locus_tag="VNU03_25710"
     CDS             complement(4216..4827)
                     /locus_tag="VNU03_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWP77944.1"
                     /translation="MIGARVLAAGALAISCLAAGPTAHAAPAGAAGLRSGAFDPPRPA
                     PEFALPSIGGAEFRLSRQLGKVIVLTFGYTSCPDICPTVLAELAQVRERLGGAAKRVQ
                     VVYVSVDPERDTPARLRAYTEQFDKTFLGLTGPADQLAPVWKAYGVSVVRRELPGTKP
                     PMYLIHHSASVFLIDPAGRLRVMAPFGTPVDDVLHDIRTLLNE"
     gene            complement(4824..5588)
                     /locus_tag="VNU03_25715"
     CDS             complement(4824..5588)
                     /locus_tag="VNU03_25715"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77945.1"
                     /translation="MRPKIEIRSISKTFVTRGGPVPVLDDLSFEVADGEFVAVIGPSG
                     CGKSTLLGVLAGFERPDRGEVLVDGEPVREPGRKGMFIFQQPSLFPWLDLERNLVFGL
                     NGESAETRRSLVAHYIALVGLEGFERAFPNQLSGGMQQRAELARALMVKPEILYLDEP
                     FGALDALTRLRMRAELLRILSRERHTVLLVTHDVEEALHLADRVLVLSPRPARIQHVV
                     EVTLPRPRALSNPELIALKASLLRELGVDEREGEPS"
     gene            complement(5595..6356)
                     /locus_tag="VNU03_25720"
     CDS             complement(5595..6356)
                     /locus_tag="VNU03_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77946.1"
                     /translation="MWSGRLAWLPLLGPVALLVAVWHVAVTLSGSLVFPTPWQVAMGL
                     GDLAWRGLLLKHVIASLFRVTCGYLLAAALAIPIGMLMGWFSTAHAFLNPLVQGLRPI
                     SPIAWIPLAILWFGVGDLSPIFLIFLSSFFPMVVGTAAGVMTIDRKYLRAARNFEYRG
                     FRLFRSVILPAALPQIVTGMRIGLGVAWLVVVAAEMIAVSSGLGYLIIDSRNAGNRYD
                     LVVAGMLTIGVVGFVLDTLMRGLERLDEVRWRYEA"
     gene            complement(6343..7224)
                     /locus_tag="VNU03_25725"
     CDS             complement(6343..7224)
                     /locus_tag="VNU03_25725"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77947.1"
                     /translation="MSRDAEGRSVVDVTLADSPAEYSKYSGWPELKESFMAGKVKAAY
                     LLAPMVMDLVESGVDAKIVSLGHRSGAVIMVRKDSPAKSFRDLRGRRIAIPSRFAVDH
                     LFVRRMLKESGMTVRDVELVEMAPPEMPAALYADQVDAYATGEPFGAVAEVGGYARPL
                     YMTRDKWPDYVCCVLTVRQELIDRDRPQIQQIVNHVLSAGAWLETAQANRDRAADIAA
                     GPTLFNQRADIIKHVLTNPRDRVTYADLRLIKSEMDELMEAALEAGIIRQRIPYERYV
                     DESFQRAARPVDIRVER"
     gene            complement(7255..7437)
                     /locus_tag="VNU03_25730"
     CDS             complement(7255..7437)
                     /locus_tag="VNU03_25730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77948.1"
                     /translation="MGPRSTWWRMFERPSLGGVLAGVAVWVVVVSALHVVVNHRGALT
                     PGAEARTLVVGGLPVT"
BASE COUNT         1184 a         2772 c         2755 g         1017 t
ORIGIN      
        1 ggcccgccgg tggcctcatc acctacagcg cgaacacgcc cgacctcttc cggcgttccg
       61 ccacctacgt ggaccggatc gtgaaaggcg cgaagcccgg cgatctgccc gtcgagcagg
      121 ccacgaggtt cgacctggtc atcgatctca aggccgcccg ggctctcggc ttgacgatcc
      181 cgcaatcgct gctgctccgg gcggaccagg tcatcgagta ggccagaccc gccggccgcc
      241 cacgcggatc gaggcgctcc tcccgcccta cgaggatctg cagctcgccc accccgacct
      301 ggtggtggag gtggaggcga tcgcggtcgt cgactgaccg gccgccgcgc gccggaccgg
      361 cccggttccc agccccggcc ccggggtgta tgctccgcgc cgtggagccc ggccggtcgc
      421 gaccgctcgg cgtcctcgcc atgctcgtca tctgggccct ggccttcggg ggggccctgg
      481 ccctctggcc ggcgcccccg cccgcccggg ccgcggacgg gcagatcacg ctcgcggtgc
      541 atatctcgct ggcccccacg tggttcgacc cggccgagac cgcgggcgtc atcaccccct
      601 ttctgatgct gtacgcgctc cacgacggcc tcgtgaagcc gatgccgggc aacgcgtgga
      661 cgcccagcct ggccgagtcg tggacgcagt cgaaggacgg gctcacctac gagttcgtgc
      721 tccgcaaggg cgtgcggttc cacaacggcg acctcctcac cgcggaggac gtgaagttct
      781 ccttcgagcg gtacaagggc gccgcggcgg gaaccctgaa ggcgcacgtc gccgcgatcg
      841 agatcgtcga cgcccaccgc gtgcgcttcc ggctcaagca gccgtggccc gactttatga
      901 cgttctacgc gacgccggcc agcggggccg cctggatcgt gcccaaggcc tatctcgagc
      961 gcgtcggcga cgacgggttc aagaaggcgc cggtcggggc cggcccctac cgcttcgtgt
     1021 ccttcagccc cgggatcgag ctggtgctgg aggcccacga cggctactgg cggaaggcgc
     1081 cggcggtgaa gcgcctcgtg ttcaagtcga tccccgacga ctcgacgcgc ctggccatgc
     1141 tcaagcgcgc cgaggccgac gtggcgtact cgctgcgcgg cccgctcggg gaggaggttc
     1201 ggcggacccc gggcctcacc ctcaaggtca cctacccgac cttcacccag tggctcacgt
     1261 tcacccagca gtgggatccg aagtcgccct gggccgatca gcgggtgcgg ctcgcggcga
     1321 acctcgccat cgaccggaag accctgagcc aggcggagta tctcgggttc ggcaaggcgg
     1381 cctccagcat catcccccgc gagtacgagt tctactgggc gccccccgag tacccgcacg
     1441 atccggcgcg ggcgaagcag ctcctggccg aggcgggcta cccgaaaggc ttcgacgcgg
     1501 gcgaggttgc caccgacgtg gtgttcgcgc cggaggccga ggcggtcatc aacggcctgc
     1561 ggtccgtcgg catccgggcg cggctgcgcc cgatggagcg ggccgcgttc tacaaggcgg
     1621 accaggacaa gcagttcaag cacctgatcc gggtgggcag cggcaccgcc ggcaacgcgg
     1681 cgacccgcat cgaggcgttc gtgctctcga gcgggatccg ctcgtacgga ggctatcccg
     1741 acatcgacgc gctcttccgg gaccaggccg ccgagcccga ccgcaagcgc cgcgaggcgc
     1801 tgctccaccg gatccagcag ctcatgcacg agcgcgcgat gttcgcgccg atcgtggagc
     1861 cgccgctgct gaccgggatc ggcccgcgcc tggcagaggt acccacgatc cccgggcacc
     1921 cctacctctc gccctacgag gatctgcgcc tcaagtgacc cggcccgcgg gcctccgctc
     1981 gatggccgcc gcgatctcgc gcccgagtgc ggcgccccgc tccgcgaact cgcggcagag
     2041 cccggtgtag cgggcggggt cgagcagcgt ctccatctgc gacggggtga gccccgcgct
     2101 cacgtgcggg tcgagcgcca gcatctcccg gaacgggcgc gactcggtca ccgaggcctg
     2161 cgccgcctcg tagaccgcgt cgtgcgcgcg ctgccgcccg atctgccgac ccagctcgag
     2221 catcagcgcc tcggccatga tcaggccgcc cgagaggtcg aggttctcgc gcatgcgatc
     2281 cgggaagacc tggagcccgt cgaagagcac catcatccgc tgcagcagat cgccggtcag
     2341 ctcgcacgcc tgcacgagcg cgcggctcat catgatgctg gtggtgcggt cggcctcgtg
     2401 ctcggtctgc atggcctcga gggccagcgg caccagcgcc cgcacctgcg cggcggccgc
     2461 gatgatgtcc tgcgagagct tgggattgcg cttctgcggc atcgtgctgc tgccgacggt
     2521 gccgggcggc accggctcct cgacctcgcc gaactcctgc ttcatgaggg tgtagacctc
     2581 acggccgatc ttgctgcacg tcgaggcgag catgccgagg agcgtgacgt actcggcctg
     2641 gtgatcgccg atggtgcgcg cgggcagccg catcgggacc atgccgaggt gcgccgccat
     2701 cttctcctgg gtggccaggc ccagctcgcc gagcgaggcg agggtgcccg cgccaccgcc
     2761 cagcatggcc acgaacacgc ggccctcgca accctgcagg cgatcgacgt ggcggcccag
     2821 ctcgtcgatc cacaccgcga ccttgaagcc aaaagtggcc ggcaccgcat gttgcccatg
     2881 ggtgcggccc ggcagcagca cgtcccgggt gcgctcggcc aggtccgcga gcgtggtgag
     2941 gatggcggcg agctggcgca ggaagatctc gtgcgcccgc cgcacctgca ggagctggcc
     3001 ggtctgggtg acgttctggg tcgtcgcgcc ccagtgcacg aagccgcccg cgtcgccctc
     3061 acacgcgcgg tcgagctccc acaccagcgg caccagcgga tggccggtgc gggccagacc
     3121 ctcccgcacc gcggccaggt ccaggaagga gagatgcgcc ttgcggacga tctcgcgggc
     3181 cgccgcctcc gggatgatgc ccagctcggc ttgcgcccgc gccagcgccg cctcgacatc
     3241 caaccaggac tggaaccgcg cggattcggt gaacagcgcg cggacgccgg ggtcgtcgat
     3301 ccggagggac gtgggctcct cgagcatggg cgatgtctcc agtcaggagc gggcggtgag
     3361 ctgagccagg cgccagcgca gccgttcgag gccggccgcg ggcggcgggt cgatgacgag
     3421 cgcgagcgtg cccgccgcgt ggagcgagca cgcgtactgg aaccgccccg gtttccggaa
     3481 cgggatccgg taggtctggc gggagccgag gatgatgggg ccgacctgat ggatgacgtc
     3541 gtcgtcgttg gtgagcacga gcacgtcgcg caccccgacc gtcaggtgga tcggcgaagg
     3601 caggacgctc gccgcctcgc ccgccttgag gcgcgccgcg gtgcccggcg ggatcacgaa
     3661 aatgagcgcg tgagcgccct ccggcagcgg cgcgagggcg gcccaggcga gcgcgacgag
     3721 agcgagcgcg aggcccgccg ccgccgcgag cgccgcgggc cgacgggtca ccgcacctcc
     3781 gcccgcgtcg tcatccgccc gccccgctcg aactcgagga tgagggtgac ggtctggccc
     3841 ggagtcagcg cgcgcttgag cccgatgagc atcagatgat aggagcccgg cttcagctcg
     3901 acgcgcgcgc cgggagccag ctcgaccttg tcgacccgct ccatcatcat catccccgac
     3961 atgttgcggg tctcgtgcag ctcgacccgc gccgcgacgt cggccgaggc cgcgaccagg
     4021 gcatcgcggt cgggcccgcg attgagcagg atgacgtagc ccgccgcggt gctctccgcg
     4081 cccggccgcg cgtccggcat ggccggggct cggcggaccc agggcttctc cagacggatg
     4141 gtggcttcct gggcaacggc ggccggagcc acgaccaggc cggccagcgc gagaacgagc
     4201 agtcggcgca tcggctcact cattgagcag ggtgcggatg tcgtgcagca cgtcgtccac
     4261 cggagtcccg aagggcgcca tcacgcgcag gcggccggcc gggtcgatca ggaacacgga
     4321 cgcggagtga tggatcaggt acatcggcgg cttggtgccg ggcagctcgc gccggaccac
     4381 cgagaccccg taggccttcc acaccggggc cagctgatcc gcgggtccgg tgagcccgag
     4441 gaaggtcttg tcgaactgct cggtgtaggc gcggaggcgc gccggggtgt cccgctcggg
     4501 gtcgacgctc acgtagacca cctgtacccg cttcgccgcc ccgccgagcc gctcccgcac
     4561 ctgagccagc tcggccagca cggtcgggca gatgtcgggg cagctcgtgt acccgaaagt
     4621 cagcacgatg accttgccga gctggcggct cagccggaac tcggcgccgc cgatcgaggg
     4681 cagcgcgaac tcgggcgccg gccgcggcgg atcgaaggcg ccgctgcgga gcccggccgc
     4741 gccggcgggc gccgcgtggg cggtcgggcc cgccgccagg caggagatcg cgagcgcgcc
     4801 ggccgcgagg acgcgcgcgc cgatcacgaa ggctccccct cgcgctcgtc cacgccgagc
     4861 tcgcgcagga gcgaagcctt gagcgcgatc agctccgggt tcgagagcgc gcgggggcgc
     4921 gggagcgtca cctcgacgac gtgctggatg cgggccggcc gcggcgagag cacgagcacg
     4981 cgatcggcca ggtgcagcgc ctcctccacg tcgtgggtca ccagcagcac ggtgtggcgc
     5041 tcgcgcgaca ggatccggag cagctcggcc cgcatgcgga gccgggtcag cgcgtcgagc
     5101 gcgccgaacg gctcgtccag gtagaggatc tccggcttca ccatcagggc ccgcgccagc
     5161 tcggcccgct gctgcatgcc accggagagc tggttgggaa aggcgcgctc gaacccctcg
     5221 agcccgacca gcgcgatgta gtgcgcgacc aggctccgtc gcgtctcggc ggactcgccg
     5281 ttcaggccga agaccaggtt gcgctccagg tcgagccagg ggaagagcga cggctgctgg
     5341 aagatgaaca tgcccttgcg cccgggctcc cgcaccggct caccgtcgac caggacctcg
     5401 ccccggtcgg ggcgctcgaa gccggcgagc acgccgagga gcgtcgactt cccgcagccc
     5461 gacgggccga tgaccgcgac gaactcgccg tcggccacct cgaagctcag gtcgtcgagc
     5521 accggcaccg gccccccgcg tgtcacgaag gtcttggaga tcgaccggat ctcgatcttg
     5581 ggcctcatgg gatctcaggc ctcatagcgc cagcgcacct cgtcgaggcg ctcgaggcca
     5641 cgcataaggg tgtcgagcac gaacccgacc acgccgatcg tgagcatgcc ggccaccacg
     5701 aggtcgtagc ggttgcccgc gttgcgcgag tcgatgatca ggtaaccgag gccggagctc
     5761 accgcgatca tctccgccgc caccaccacg agccaggcga ccccgagccc gatgcgcatg
     5821 ccggtcacga tctggggcag cgcggcgggc aggatcaccg agcggaagag ccggaagccc
     5881 cggtactcga agttgcgggc ggcccgcagg tacttgcggt cgatcgtcat cacccccgcc
     5941 gcggtcccca ccaccatggg aaagaatgac gagaggaaga tcaggaagat cggcgagagg
     6001 tcccccaccc cgaaccacag gatcgcgagc gggatccagg cgatcggcga gatcgggcgg
     6061 agcccctgca cgagcgggtt caggaaggcg tgggcggtgg agaaccagcc catcagcatg
     6121 ccgatcggga tggcgagcgc ggcggccagg aggtagccgc acgtgacgcg gaagagcgac
     6181 gcgatcacgt gcttgagcag gagaccgcgc caggcgaggt ctccgaggcc catcgccacc
     6241 tgccagggcg tcgggaagac caggctgccg ctcagcgtga ccgccacgtg ccagaccgcg
     6301 accagcaggg ccaccgggcc cagcaggggc agccaggcga gcctaccgct ccacacggat
     6361 gtccaccggg cgcgcggcgc gctggaagct ctcgtccacg taccgctcgt aggggatgcg
     6421 ctggcggatg atgccggcct cgagcgcggc ctccatcagc tcgtccatct cggatttgat
     6481 gagccgcagg tccgcgtagg tcacgcggtc gcggggattg gtgagcacgt gcttgatgat
     6541 gtcggcgcgc tggttgaaca gggtggggcc ggccgcgatg tcggcggccc ggtcgcggtt
     6601 ggcctgcgcg gtctcgagcc acgcgccggc ggacagcacg tggttgacga tctgctggat
     6661 ctgcggccgg tcgcggtcga tcagctcctg gcgcaccgtg agcacgcagc acacgtagtc
     6721 gggccacttg tcgcgcgtca tgtagagggg acgcgcgtag ccgcccacct ccgccacggc
     6781 cccgaacggc tccccggtcg cgtaggcgtc gacctggtcc gcatagaggg cggccggcat
     6841 ctcgggcggc gccatctcca ccagctccac gtcacggacg gtcatgccgc tctccttgag
     6901 catgcggcgc acgaagaggt ggtcgaccgc gaaccggctc gggatcgcga tgcgccggcc
     6961 gcgcaggtcg cggaagctct tcgcgggcga atccttcctc accatgatga ccgcgcccga
     7021 gcgatgaccg agcgacacga tcttggcgtc caccccgctc tccacgagat ccatcaccat
     7081 cggcgcgagc agataggccg ccttgacctt gccggccatg aacgattcct tcagctcggg
     7141 ccacccgctg tacttgctgt actcggccgg ggagtcggcg agggtcacgt cgaccaccga
     7201 ccggccctcc gcgtcccgcg acatcgccac actggcgatc ggcagcgtga ggtttcacgt
     7261 gaccggcagg ccgcccacta ccagggttcg ggcctctgcg ccgggagtga gcgcgccgcg
     7321 gtgattgacg acgacgtgca aggcggacac caccaccacc cagacggcca ccccggcgag
     7381 caccccgccg aggctcggcc gctcgaacat ccgccaccac gtcgagcgag gccccacggg
     7441 gtgaaagtca accacatttc tgccactcct ccaacccgat cggactacta tctgcggaag
     7501 caccgggacg cgtcggcaat tccgatgggc gagggcggct ggccagggag aacactatgg
     7561 gcaaactgga cgggaaggtc gcgctgatca gcggcggagc tcgggggcag ggcgcggcgg
     7621 aggcggagac cttcgcgcgc gagggcgccc gggtcgtgtt cggggacatc cgggacgacg
     7681 aggggaagaa ggtcgaggcg ggcatccggg cggcgggcgg cgacgcca
//
LOCUS       DATMCV010001122         3387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_61194, whole genome shotgun sequence.
ACCESSION   DATMCV010001122 DATMCV010000000
VERSION     DATMCV010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61194"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VNU03_25735"
     CDS             complement(<1..1485)
                     /locus_tag="VNU03_25735"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWP77949.1"
                     /translation="MAGTPPTRALTVGDIMSRSVLTTEVGASVAEAAALMYSRRVGSI
                     VVVQGNTPIGILTERDLVRFAAGAADARAALVGDHMTAEPDTVEETEEVIDAFRRFAA
                     HGYRHIPVVAGGDLVGIISMRDLVMKLRRGPAAAAHGAVGPSAAMEALPPVASDLTST
                     VLSLLEAGRATVPALVVPDGPELSYRALRQHVSRVADTLASVGVARGDRVAMVLGNGP
                     EAVVAILGAAVAAAAAPLNPACTEDELRYYLEDVSARALIVPRGGAEAARRAWPAGAP
                     LIEIAVEAGGKLWLETSAPIAKRRTATAPGPDDVALVLHSSGTTGRPKRAALRHRNLA
                     ASARNIVATYGLSSLDTALCVMPLFHIHGLVGCTLSTFASGGTVVMPVRFNPVGFRRI
                     LSAHEPTWYTAVPTIHQLILARHRDRAHRGSSLRFLRSASSKLHEATLLGLEEAFGVP
                     CLEAYGMTEASHQIASNPLPPASRVSGSVGRGVGVRIGVMDTHGQ"
     gene            1554..2825
                     /locus_tag="VNU03_25740"
     CDS             1554..2825
                     /locus_tag="VNU03_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-binding protein"
                     /protein_id="HWP77950.1"
                     /translation="MPLLGPITFPTMYRVRPAFARTRLEDIPAGVAATLAAGRLPIKR
                     GDTVAVGAGSRGIANIAMIVRATVDWLKAQGARPFVFPAMGSHGGATAEGQRSVLAHY
                     GITEATMGCEVRASMDVIQVGEALGLPVWLDRIASEADWIGLVNRIKPHTDFKGSIES
                     GLFKMMTIGLGKYQGATQYHRANVNHGYETVITAVGREMLAKARIGFGLGIVENGYDE
                     TACVEAFNASDLEAGERRLLKDARAWMARLPFGRLDVLVVEEIGKNISGSGMDTNVIG
                     RPSNPHEPFPADPKILWIVALDLTDGSEGNATGIGNADFTTRRLAEKIDWKPTAINTL
                     TACAPNGAKLPLVFESDREAVENALNCIGLTPPEQARVVRIRSTLVLGEIECSEALLP
                     EIAKRPDLEVVGPGRPLPFDAAGRIVPLLAH"
     gene            2954..>3387
                     /locus_tag="VNU03_25745"
     CDS             2954..>3387
                     /locus_tag="VNU03_25745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77951.1"
                     /translation="MGPHNRRQFLTTTGAGLAGILATRVAPARGQQREISYLCWNNFA
                     PASDKKLAEIGQRFSKDTGIKLKIDHISHMGPQQAKYASEVQTQAGHDMVEMRMHFPW
                     LYEPQLVDVSDVVAGLEKQYGKAVGSAYEGAHVNGVWRAVPQ"
BASE COUNT          504 a         1204 c         1229 g          450 t
ORIGIN      
        1 ctggccatgg gtgtccatca ccccgatgcg cacgccgacg ccacgcccga ccgagccgga
       61 cacgcgcgag gcggggggca gggggttcga ggcgatctgg tgcgaggcct cggtcatgcc
      121 gtaggcctcg aggcacggca ccccgaacgc ctcctccagc ccgagcaggg tcgcctcgtg
      181 gagcttggag ctggccgagc ggaggaagcg cagcgagctg ccacggtgcg cgcgatcgcg
      241 gtggcgcgcc aggatgagct ggtggatggt cggcaccgcg gtgtaccagg tcggctcgtg
      301 cgcgctgagg atgcgccgga agcccaccgg gttgaagcgc accggcatca ccaccgtgcc
      361 gccggacgcg aaggtggaga gcgtgcagcc caccaggccg tggatgtgga agagcggcat
      421 cacgcagagc gcggtgtcga gcgaggagag cccgtaggtc gcgacgatgt tgcgggccga
      481 ggccgcgagg ttgcggtgcc gcagcgctgc gcgcttcggg cggccggtgg tgccactgct
      541 gtgcagcacc agcgccacgt cgtccgggcc gggagcggtc gcggtccgtc gcttcgcgat
      601 cggggccgat gtctccagcc agagcttgcc gcccgcctcg accgcgatct cgatcagcgg
      661 cgcgcccgcc ggccaggccc ggcgcgccgc ctccgcgccg ccgcggggca cgatgagggc
      721 gcgggccgag acgtcctcga gatagtagcg gagctcgtcc tccgtgcagg ccgggttgag
      781 cggcgcggcc gcggccgcga ccgccgcgcc gaggatcgcc acgaccgcct ccggcccgtt
      841 gcccaggacc atggcgacgc ggtcccctcg cgccacgccc acggaggcga gcgtgtcggc
      901 gacccggctc acgtgctggc gcagggcgcg gtacgagagc tccgggccgt ccggcaccac
      961 cagcgcgggg acggtcgcgc gcccggcctc gaggagggac agtacggtgc tggtcaggtc
     1021 ggacgccacg gggggcagcg cttccatcgc cgcgctcggg cccaccgcgc catgggccgc
     1081 cgcggcgggg ccgcggcgga gcttcatgac caggtcgcgc atcgagatga tgccgaccag
     1141 gtcgccgccc gccaccaccg ggatgtgccg gtagccgtgc gcggcgaacc gccggaacgc
     1201 gtcgatgacc tcttccgtct cctcgacggt gtccggctcc gcggtcatgt ggtcgcccac
     1261 gagcgcggcg cgggcgtcgg cggcgccggc cgcgaaccgg accaggtcgc gctcggtgag
     1321 aatgccgatc ggggtgttgc cctggacgac cacgatcgac ccgacccgcc gcgagtacat
     1381 cagggcggcc gcctccgcga cgctcgctcc cacctcggtg gtcagcaccg agcggctcat
     1441 gatgtcgccc accgtcaggg cgcgggtcgg aggtgtgccg gccatgtgtc tcatcctagc
     1501 ccagttgtcc gccgccgttg acagggcccg ggccggctac atatcatcgc ggcatgcccc
     1561 ttctcggacc gatcaccttt cccacgatgt accgcgtgcg accggcgttc gcgcgcacgc
     1621 ggctcgagga catcccggcc ggggtcgccg cgaccctcgc ggcgggtcgc ctgccgatca
     1681 agcgcggtga cacggtggcg gtcggcgcgg gcagccgcgg catcgcgaac atcgcgatga
     1741 tcgtgcgcgc cacggtggac tggctgaagg cccagggggc gcggcccttc gtgtttccgg
     1801 ccatgggcag tcacggtggg gccaccgcgg agggccagcg gtcggtcctg gcccactacg
     1861 gcatcaccga ggcgaccatg ggctgcgagg tacgggcctc catggacgtg atccaggtcg
     1921 gggaggcgct cgggctgccg gtctggctcg accggatcgc ttcggaggcg gactggatcg
     1981 ggctggtcaa ccggatcaag ccgcacaccg acttcaaggg atcgatcgag tccggcctct
     2041 tcaagatgat gacgatcggc ctcggcaagt accagggtgc gacccagtac caccgggcca
     2101 atgtcaacca cggctacgag accgtgatca ccgcggtggg ccgggagatg ctggccaagg
     2161 cccggatcgg cttcgggctc gggatcgtgg agaacggcta cgacgagacc gcgtgcgtcg
     2221 aggccttcaa cgcgtcggat ctggaagcgg gggagcggag gctgctcaag gacgcgcgcg
     2281 cctggatggc ccggctgccc ttcgggcggc tcgacgtgct ggtggtcgag gagatcggca
     2341 agaacatctc cggctccggc atggacacca acgtgatcgg ccgcccgtcc aatccccacg
     2401 agccgttccc ggccgatccc aagatcctct ggatcgtcgc gctcgacctc accgacggga
     2461 gcgagggcaa cgccaccggg atcggcaacg ccgacttcac gacccggcgg ctcgccgaga
     2521 agatcgactg gaagcccacc gcgatcaaca ccctcaccgc gtgcgccccc aacggggcga
     2581 agctgccgct cgtgttcgag agcgaccggg aggcggtgga gaacgcgctc aactgcatcg
     2641 gcctcacccc gcccgagcag gcgcgggtgg ttcgcatccg cagcaccctc gtcctcggcg
     2701 agatcgagtg ctccgaggcc ctgctgcccg agatcgccaa gcggccggac ctcgaggtcg
     2761 tgggaccggg tcggccgctc cccttcgacg cggccggccg gatcgttccg ctgctcgctc
     2821 attgagcggc cgccggcggg gcctgccgcg ccggggccct tgtggcttga cacgcagcgc
     2881 ccgggcagcg tagagtccgc gaaaccccag atcccgtgtc acgacccgtg tcctcaccga
     2941 ggaggagccg cccatgggac ctcacaaccg ccgtcaattc ctgaccacga ccggggccgg
     3001 tctcgccggc atcctcgcca cgcgcgtggc gcccgcccgg ggccagcagc gcgagatctc
     3061 gtatctctgc tggaacaact tcgctcccgc gtccgacaag aagctggccg agatcggcca
     3121 gcgcttctcc aaggacaccg ggatcaagct caagatcgac cacatctccc acatgggtcc
     3181 gcagcaggcc aagtacgcct ccgaggtcca gacccaggcc gggcacgaca tggtggagat
     3241 gcggatgcac ttcccgtggc tctacgagcc gcagctggtg gacgtctcgg acgtggtggc
     3301 cggcctcgag aagcagtacg gcaaggcggt cggctccgcc tacgagggcg cgcacgtcaa
     3361 cggggtctgg cgcgcggtgc cccagta
//
LOCUS       DATMCV010001123         3277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_730779, whole genome shotgun sequence.
ACCESSION   DATMCV010001123 DATMCV010000000
VERSION     DATMCV010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730779"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2373
                     /gene="glnD"
                     /locus_tag="VNU03_25750"
     CDS             <1..2373
                     /gene="glnD"
                     /locus_tag="VNU03_25750"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HWP77952.1"
                     /translation="SDLDLMLIYDGEMGPYVQRVTQGLLYALWDLGLQVGHAVRSLPD
                     CLAMARTDFPSRTSMQQARFLVGDRRLFNRFRKVLAENVYQKDFAQFLETTLTERDQR
                     YRKFGGSPYMGEPNVKESAGGLRDIHTAMWLASTKFGTRTLRELLDKRLITAREQKAS
                     DEALTFLWRVRNELHFLSGHKNDVLSRDVQPQIAKNFGYTADELSLGVEKFMRDYYLH
                     ARVIHRVSRRLIARCRETLSRRATVQRRLRQEALADGLVVIDEQLHLAQPDGRAFREE
                     PLRLLKVFWHRHQLGFEMGIDVERAIEESLDLIDDAFRASAEARDLFLGICRNWGRVA
                     QTFREMHELGVLGRYLTEWDALTCLVQYDVYHKFSADQHSLIAVQNLEALAPGQSADS
                     EGNAQVVSELERPGLLMMGMLLHDIGKGRGHGHVAKGIPLIEALTARIGLAPEDADKV
                     IFLVAHHLTMSHIAQRRDIDDPKTIESLAEVCRTPERLRMLYLLTCADMRAVGPGVMT
                     GWQAQILWDLYARTLAHLTGGQRERPTREAVALRVTAAMRGEVPRTTLAAHLAMLSER
                     YLATTSPQRIAAHLRLLDRPGEEGVLATELFHHPDLGSSELVVATRDVPGLFSLIAGT
                     LAAQGINILSAQIHTRADGIVIDTFQVNDPFGEAVTEEARWRRTLEALRRVLRGEASV
                     EELLARRRAVHAGPEGVAGPPKISLDNQLSDSHTVLEVKCPDRVGLLYVITRTLSAQG
                     LDIASARIATEIDQAYDTFYLTDRQGRRLEDDPAMGRVRESLEDALLKPF"
     gene            2375..3055
                     /locus_tag="VNU03_25755"
     CDS             2375..3055
                     /locus_tag="VNU03_25755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWP77953.1"
                     /translation="MLSHYKTPLSRLGDPVARVLLQAHVRPNHLTVLGLGVSIVAAGA
                     LAGGRLRMGAVLLTLAGLFDFFDGSLARLANRVSAFGAFLDSVVDRYSDLVVLLGIVL
                     FYHRAADVQGVFLTMITVIGTVMVSYTKARAQSIGLSCEIGLMERPERLIVLIAGATF
                     NLLTPAMIVLAVLTNLTALQRILYTRAAFTQSAPAPSSVPVVAPVAAPAPVRAPAEPD
                     VTPLRPSA"
BASE COUNT          464 a         1220 c         1111 g          482 t
ORIGIN      
        1 tccgatctcg acctgatgct gatctacgac ggcgagatgg gcccgtacgt gcagcgggtg
       61 acccagggcc tgctctacgc cctctgggac ctgggcctgc aggtcggcca cgcggtgcgc
      121 agcctgcccg attgcctggc catggcccgc accgacttcc cgagccgcac ctcgatgcag
      181 caggcgcgct tcctggtggg cgaccggcgc ctcttcaacc gcttccggaa agtgctcgcg
      241 gagaacgtct accagaagga cttcgcccag ttcctcgaga ccaccctcac cgagcgcgac
      301 cagcgctacc gcaagttcgg cgggtcgccc tacatgggcg agcccaacgt gaaggagtcg
      361 gcgggagggc tgcgcgacat ccacaccgcg atgtggctcg cctccaccaa gttcggcacc
      421 cggaccctgc gcgagctcct ggacaagcgg ctcatcaccg cgcgcgagca gaaggcgagc
      481 gacgaggcgc tcaccttcct gtggcgggtg cgcaacgagc tgcacttcct ctccgggcac
      541 aagaacgacg tgctctcgcg cgacgtccag ccccagatcg ccaagaactt cggctacacc
      601 gccgacgagt tgtcgctcgg ggtcgagaag ttcatgcgcg actactatct ccacgcgcgg
      661 gtgatccacc gggtgtcccg gcgcctgatc gcccgctgcc gcgagaccct ctcgcgtcgc
      721 gccaccgtgc agcggcgcct gcgccaggag gcgctcgccg acgggctcgt cgtcatcgac
      781 gagcagctgc acctggccca gccggacggg cgcgccttcc gcgaggagcc gctgcggctc
      841 ctcaaggtgt tctggcatcg ccatcagctc ggcttcgaga tgggcatcga cgtggagcgc
      901 gccatcgagg agtccctcga tctcatcgac gacgcgttcc gcgcctcggc ggaggcccgc
      961 gacctcttcc tcggcatctg ccgcaactgg ggccgggtcg cccagacctt ccgcgagatg
     1021 cacgagctgg gagtgctggg ccggtacctg accgagtggg acgcgctgac ctgcctcgtg
     1081 cagtacgacg tctaccacaa gttctccgcc gaccagcact cgctgatcgc ggtgcagaac
     1141 ctggaggccc tcgcgccggg ccagtcggcg gactcggagg gcaacgcgca ggtggtgagc
     1201 gagctggagc gtccgggatt gctgatgatg ggcatgctcc tgcacgacat cggcaagggg
     1261 cgggggcacg gacacgtggc caagggcatc ccgctcatcg aggccctcac cgcgcgcatc
     1321 gggctcgccc ccgaggacgc cgacaaggtg atcttcctgg tggcccacca cctgaccatg
     1381 tcgcacatcg cccagcggcg cgacatcgac gacccgaaga cgatcgagtc gctggccgag
     1441 gtctgccgca cgccggagcg cctgcgcatg ctctacctgc tcacctgcgc ggacatgcgc
     1501 gcggtgggcc ccggggtgat gaccggctgg caggcccaga tcctctggga cctctacgcg
     1561 cgcaccctcg cgcacctcac cggcgggcag cgcgagcggc ccacccgcga ggcggtggcc
     1621 ctgcgggtga ccgcggccat gcgcggcgag gtgccgcgca ccacgctggc cgcccacctg
     1681 gcgatgctct ccgagcgcta cctcgccacc acctcgcccc agcgcatcgc ggcccacctg
     1741 cggctgctcg accgccccgg cgaggagggg gtgctcgcca ccgagctctt ccaccatccc
     1801 gacctcggct cctccgagct ggtcgtggcc acccgcgacg tgcccgggct cttctcgctg
     1861 atcgcgggca ctctcgccgc gcagggcatc aacatcctct cggcccagat ccacacccgg
     1921 gccgacggca tcgtcatcga caccttccag gtgaacgatc cgttcgggga ggcggtcacc
     1981 gaggaggcgc gctggcgccg gacgctcgag gccctgcgtc gcgtgctgcg cggcgaggcg
     2041 agcgtggagg agttgctcgc ccggcggcgg gcggtgcacg cgggcccgga gggcgtggcg
     2101 gggccgccca agatctcgct cgacaaccag ctctccgaca gccacaccgt gctggaggtc
     2161 aagtgccccg accgggtggg gctgctctac gtcatcaccc gcacgctctc ggcccagggc
     2221 ctcgacatcg cgagcgcgcg catcgccacc gagatcgatc aggcctacga cacgttctac
     2281 ctgaccgacc ggcagggccg gcgcctcgag gacgacccgg cgatgggccg ggtgcgcgag
     2341 agcctggaag acgccttgct gaagccgttc tgagatgctg agccactaca agaccccgct
     2401 ctcacggctc ggcgacccgg tcgcgcgggt cctgctccag gcccacgtgc ggccgaatca
     2461 cctgaccgtg ctcggcctcg gggtcagcat cgtcgcggcc ggcgcgctcg ccggggggcg
     2521 cctgcggatg ggcgcggtgc tgctcaccct cgccgggctc ttcgacttct tcgacggctc
     2581 gctcgcgcgg ctcgccaacc gcgtgagcgc gttcggcgcc ttcctcgact cggtggtgga
     2641 ccgctactcg gacctggtgg tgctgctcgg gatcgtgctc ttctaccacc gggccgccga
     2701 cgtccagggc gtcttcctca ccatgatcac cgtgatcggc accgtcatgg tcagctacac
     2761 gaaggcgcgc gcccagtcca tcgggctctc ctgcgagatc gggctcatgg agcgccccga
     2821 gcggctgatc gtgctgatcg cgggggccac cttcaacctg ctcaccccgg ccatgatcgt
     2881 gctggcggtg ctcaccaacc tgaccgcgct ccagcggatc ctgtacaccc gcgcggcgtt
     2941 cacgcagtcg gcgcccgcgc cgtcgagcgt gccggtggtg gcgccggtcg cggcgcccgc
     3001 cccggtgagg gcccccgccg agcccgacgt gacgcccctg cggccgtcgg cctaggcgag
     3061 ccccgtcgac attccggtac acggggtcca cgccgggtgc ttggtacact ggtgaccatg
     3121 aagggcctca ccgcgggcgt gtgcatggtg tcggtcgtgc tcctgggcgc ggcacccgcg
     3181 gggctgcctc ccgacatgcg ggagcggttc accgcggcgg tggccaccca caaggcgggc
     3241 gactggagcg cggcggccaa ggagttcggc gatccgg
//
LOCUS       DATMCV010001124         6059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_730857, whole genome shotgun sequence.
ACCESSION   DATMCV010001124 DATMCV010000000
VERSION     DATMCV010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730857"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VNU03_25760"
     CDS             <1..437
                     /locus_tag="VNU03_25760"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWP77954.1"
                     /translation="RLSALLQANFTGTVLLCQQAAERLAARGGGLICAFGSVAGDRAR
                     RSNYLYGASKAGLAVFLDGLDLAYRERGVRALCVKPGFVRTAMTAGLPVPPFAGEPDA
                     VARRVLAAMDAGRHDVYAPGVWRFVMLLIRALPRAVLRRVQF"
     gene            449..814
                     /locus_tag="VNU03_25765"
     CDS             449..814
                     /locus_tag="VNU03_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77955.1"
                     /translation="MPKIWRVYSGKDGESHVEEMPLAMKPFTDTEGAHGEGTALQPAA
                     GIVFRVSPPGYVLDWHCAPRRQYSITLSGEAEIQVGDGTVVRVGPGDVLLAEDLTGRG
                     HVTRSIGSGPRFYAIVPLP"
     gene            complement(828..1568)
                     /locus_tag="VNU03_25770"
     CDS             complement(828..1568)
                     /locus_tag="VNU03_25770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77956.1"
                     /translation="MSPISPAIRSDFAPTGTMRVGINHGNLVLAQKNPTTGEVKGVAV
                     DMAHELGRRLGVPVELVQYTSAGQMTDALATGAWDVAFLAREPGRAGEIGFTAPHLLI
                     EGTYLVPAGSPYTRVPDVDRDGVRIAVSEKSAYDHFLTRNIKHAKLVRGAGNSAGLGL
                     LQAGQADVIAGVRQLLLMDQAKLPGSKVLEDRFMVIEQAMGMPRGRDAGLQYAKEFIE
                     DVKASGFVARSLEKSGVRGVPVAPPEKP"
     gene            complement(1625..2818)
                     /locus_tag="VNU03_25775"
     CDS             complement(1625..2818)
                     /locus_tag="VNU03_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007119828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWP77957.1"
                     /translation="MSADVVLRGVRPMAAGGPVDVLVRAGVIERVAPGIVPPAGASVV
                     DGRDGILLPALVDAHMHLDKTFWGLPWRAHEEGPTTRERIENERRLRRELALPADVQA
                     ERLVRQAVSRGTLHIRSHVDVDTEIGLRNFEGVMTARGRLRDTVSIQVVAFPQSGVVT
                     RPGTAELLEQAVKDGAELIGGVDPTGIDRDAAGQLDVVFGIAGRYGAGVDVHLHDGGE
                     EGAAQIRMIAERTRALGLAGKVAVSHAFCLGMVDEPELEGLLALLREQRIAIMTTAPG
                     DRPTPPVRRLREAGVTVGAGSDGVRDAWTPLGNADMLERAMLVAYRNGFRADPLLHDA
                     LDIATRGNAGVLGLEGYGLEVGCRADLVVVPGETLGEIVAMRPPRALVISGGRVVARD
                     GQCAA"
     gene            2969..4570
                     /locus_tag="VNU03_25780"
     CDS             2969..4570
                     /locus_tag="VNU03_25780"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77958.1"
                     /translation="MRPVQSWKWLTLVLVALATVAPGGLAIRPAAAQGGGTLVVGLDQ
                     EPPTLDPHASPSAVTYQVIASVAENLLYRGTDGKLVPWLAESWTVARDGRSVTFKLRR
                     DVKFHDGTPFNADAVKFNFDRIVDPKFKAGGSRAALGSYAASKVLDEFTVQVLFETPY
                     APFLNSVASGVLSIVSPKAVRESGDAVHTRPVGTGPFMMKDYVAKDHATMVRFPGYAR
                     KAPWSDRTGPALLETVVWKFIPEPGTRVTTLESGETQGIYLVPAQSLPRLEKDASVRV
                     EKLPWPGVPRIWLLNTTKPPFDDVRVRRAVNFAVDKDAFLATVYRGTGLKAFAPLTAV
                     MLDDPALRQAYPFDPEKAKALLGEAGWAPGGDGIRAKAGQRLEIVLNAIEYGGGADPT
                     AQLIQSSLRDVGVDVKIKAQARPPWYEDNYRCATHGPVMFLRSIDPDGLWSLFHSSQV
                     GANFNWSCVKNAKLDGLLADGRREADPAKRRAIYLAVEKLALDEALTVPLVDELAVWA
                     FRAPVQGVKYNFNAYPVLSDATIRR"
     gene            4580..5503
                     /gene="nikB"
                     /locus_tag="VNU03_25785"
     CDS             4580..5503
                     /gene="nikB"
                     /locus_tag="VNU03_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HWP77959.1"
                     /translation="MLTYVGRRILAVVPVLFGVTLAVFSMLFLVPGDPVKIMLAEFVT
                     TPDQIAQMRAQLHLDEPVLKQYGRFVTNALRGDLGVSIRSRRPVAAEITENIGSTGQL
                     ALASMLVAIGLGVPLGLLAALGRNSWLDVVAMVVALLGVAMPSFWLGFLLIFVFSLHL
                     GWLPATGGGDLPHLVMPAVALGMIAAAIIARLTRSSMLEVLGQDYVRTARAKGLGRAS
                     VIVRHALRNALIPVVTVFGLQFGNLLAGAVIVETVFSRPGLGRLIVGGILAKDFPLVQ
                     GTVLFVAAAYVLINVVVDVAYAYVDPRIRIG"
     gene            5500..>6059
                     /locus_tag="VNU03_25790"
     CDS             5500..>6059
                     /locus_tag="VNU03_25790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77960.1"
                     /translation="MSAAAGLLRHRGALIGLVILGALATMAVAAPWISARDPIRTAPR
                     EALQAPGARFVLGSDQFGRDVASRVLYGARVSLLVGVISVSIAVGLGAPLGLVSGYYG
                     GRLDALIMRVMDVMLAFPGILLALAIVSVLSPGLGNVMIAVGLSAVPGYARLVRATVL
                     SAREHLYVEAARALGGRDGGILVRDIL"
BASE COUNT          771 a         2217 c         2191 g          880 t
ORIGIN      
        1 cgcgcctctc cgcgctgctc caggccaact tcaccggcac ggtgctgctc tgccagcagg
       61 cggccgagcg gctggccgcg cggggcggcg ggctcatctg cgccttcggc agcgtggcgg
      121 gcgaccgcgc ccggcgcagc aactatctct acggggcgtc gaaggcgggg ctcgcggtgt
      181 tcctcgacgg gctcgacctg gcctaccgcg agcgcggggt gcgcgcgctc tgcgtgaagc
      241 ccggcttcgt ccggaccgcg atgaccgcgg ggctgccggt gccgcccttc gcgggcgagc
      301 ccgacgcggt ggcccgccgc gtgctggccg caatggacgc gggccgtcac gacgtctacg
      361 ctccgggcgt gtggcgcttc gtcatgctgc tcatccgcgc gctgccgcga gcggtgctcc
      421 ggcgcgtgca gttctgagag agggagcgat gcccaagatc tggcgggtct acagcgggaa
      481 ggacggcgag tcccacgtcg aggagatgcc gctcgccatg aagccgttca ccgacaccga
      541 gggcgcccac ggggagggca ccgcgctgca gcccgccgcg ggcatcgtgt tccgggtctc
      601 gccgccgggc tacgtgctgg actggcactg cgccccgcgc cggcagtact cgatcacgct
      661 ctccggggaa gcggagatcc aggtcggcga cggcaccgtg gtccgcgtcg ggccgggcga
      721 cgtgctgctg gccgaggacc tcaccgggcg aggccacgtc acccgctcga tcgggagcgg
      781 gccccgcttc tacgccatcg tcccgctccc ctgagcggac gccgcgctca gggcttctcg
      841 gggggcgcca ccggcacccc gcgcaccccc gacttctcga gcgaccgggc cacgaagccg
      901 gacgccttca cgtcctcgat gaactccttc gcgtactgca ggcccgcgtc gcgcccccgc
      961 ggcatcccca tcgcctgctc gatcaccatg aagcggtcct cgagcacctt cgagcccggg
     1021 agcttggcct ggtccatcag gaggagctgg cgcaccccgg cgatcacgtc ggcctgaccc
     1081 gcctgcagca gcccgagccc cgcgctgttg cccgccccgc gcaccagctt cgcgtgcttg
     1141 atgttgcggg tcaggaagtg atcgtaggcg ctcttctcgg agaccgcgat gcgcaccccg
     1201 tcgcggtcca cgtcgggcac gcgcgtgtag ggcgatccgg ccggcaccag ataggtcccc
     1261 tcgatcagca ggtgcggggc ggtgaagccg atctcgcccg cccggcccgg ctcccgggcc
     1321 aggaaggcca cgtcccacgc gccggtggcg agcgcgtcgg tcatctggcc ggccgaggtg
     1381 tactggacca gctcgaccgg gacgccgagc cggcggccca gctcgtgggc catgtcgacc
     1441 gcgacgccct tcacctcgcc ggtggtgggg ttcttctggg cgaggaccag gttgccgtgg
     1501 ttgatgccga cccgcatggt gccggtcgga gcgaaatcgg agcggatcgc gggagagatt
     1561 ggtgacatgg tggggctggc tcctaggaga aagagggtgg cgacggttgc ggtgacatgc
     1621 gacgtcaggc ggcgcactgg ccgtcccggg cgaccacgcg gcccccgctg atcacgagcg
     1681 cgcggggcgg ccgcatggcg acgatctcgc cgagggtctc gccgggcacc accaccaggt
     1741 cggcgcggca gccgacctcg aggccgtagc cctcgaggcc cagcacgccc gcgttgccgc
     1801 gggtggcgat gtcgagcgcg tcgtgcagca ggggatcggc gcggaagccg ttgcggtagg
     1861 cgaccagcat cgcgcgctcc agcatgtccg cgttgccgag cggcgtccac gcgtcgcgca
     1921 cgccgtccga gcccgcgccc accgtgaccc cggcctcgcg gaggcggcgc accggtgggg
     1981 tcgggcggtc gcccggcgcg gtggtcatga tcgcgatgcg ctgctcccgg agcagggcca
     2041 gcagcccctc gagctcgggc tcgtcgacca tgccgaggca gaaggcgtgg ctcaccgcca
     2101 ccttgcccgc gagcccgagc gcgcgggtgc gctccgcgat catgcggatc tgcgccgcgc
     2161 cctcctcgcc tccgtcgtgg agatggacgt cgacgcccgc gccgtaccgg cccgcgatgc
     2221 cgaagaccac gtcgagctgc cccgccgcgt cgcggtcgat gccggtggga tcgacgccgc
     2281 cgatcagctc ggcgccgtcc ttcaccgcct gctccagcag ctccgcggtg ccggggcggg
     2341 tgacgacccc gctctgggga aacgccacca cctggatcga gacggtgtcc cggaggcggc
     2401 cgcgcgcggt catgacgcct tcgaaattgc gcaggccgat ctcggtgtcg acgtccacgt
     2461 ggctgcggat gtggagcgtc ccgcggctca ccgcctggcg caccaggcgc tcggcctgca
     2521 cgtccgcggg cagcgccagc tcgcggcgca ggcgccgctc gttctcgatg cgctcgcgcg
     2581 tggtgggccc ctcctcgtgg gcgcgccacg gcagacccca gaacgtcttg tcgagatgca
     2641 tgtgcgcgtc gaccagggcg gggaggagga tgccgtcccg tccgtcgacg accgacgcgc
     2701 cggccggcgg cacgatgccg ggagccacgc gctcgatgac cccggcccgg accagcacgt
     2761 ccaccggccc gcccgccgcc atcggacgca caccgcgaag cacgacgtcg gcgctcatgc
     2821 tccggcgagt atcggcagcg ccgcgtgaca agtcaagcgc gcggccggcg cttcgctcca
     2881 ccttgacgcg gggcggatgg ctgacgcagg atgggccctc ggtcgtgagc gaagcgagcg
     2941 gtgccccggc gcccggagga ggagagcgat gcgtcccgtg caatcctgga agtggctgac
     3001 cctcgtcctc gtcgcgctgg ccacggtggc tcccggagga ctcgcgatcc gccccgccgc
     3061 cgcgcagggc ggcggcaccc tggtggtggg gctcgaccag gagccgccca ccctcgaccc
     3121 gcacgcctcg ccctccgcgg tcacctacca ggtcatcgcc tcggtggccg agaacctgct
     3181 ctaccgcggg accgacggca agctcgtgcc gtggctcgcc gagtcctgga ccgtcgcgcg
     3241 cgacgggcgc agcgtgacct tcaagctgcg ccgcgacgtg aagttccacg acggcacgcc
     3301 gttcaacgcc gacgcggtga agttcaactt cgaccggatc gtggacccga agttcaaggc
     3361 gggcggctcc cgcgccgcgc tcggcagcta cgccgcgtcg aaggtgctgg acgagttcac
     3421 ggtgcaggta ctcttcgaga ccccctacgc ccccttcctg aactcggtgg cctccggggt
     3481 cctctccatc gtgtcgccga aggcggtgcg cgagagcggt gacgcggtcc acacgcgccc
     3541 ggtgggcacc gggcccttca tgatgaagga ctacgtggcc aaggatcacg cgacgatggt
     3601 ccgcttcccc ggttacgcgc ggaaggcccc ctggagcgac cggaccggac ccgccctgct
     3661 cgagacggtg gtgtggaagt tcatccccga gccggggacg cgggtgacca cgctcgagag
     3721 cggcgagacc cagggcatct acctcgtgcc cgcccagtcg ctgccccggc tcgagaagga
     3781 cgcgagcgtc cgggtggaga agctcccgtg gccgggggtg ccgcgcatct ggctgctcaa
     3841 caccacgaag ccccccttcg acgacgtgcg ggtgcgccgc gcggtgaact tcgcggtgga
     3901 caaggacgcg ttcctcgcca ccgtgtaccg cggcaccggg ctcaaggcct tcgccccgct
     3961 caccgcggtg atgctcgacg atccggcgct gcgccaggcc tatccgttcg atcccgagaa
     4021 ggcgaaggcc ctgctgggcg aggcgggctg ggcccccgga ggagacggca tccgggccaa
     4081 ggcgggccag cggctcgaga tcgtgctgaa cgccatcgag tacggcgggg gcgcggaccc
     4141 caccgctcag ctcatccagt cctcgctccg ggacgtcggc gtcgacgtga agatcaaggc
     4201 ccaggcgcgc ccgccgtggt acgaggacaa ctaccgctgc gccacccacg ggccggtgat
     4261 gttcctgcgc tcgatcgatc cggacgggct gtggtcgctc ttccactcgt cccaggtggg
     4321 cgccaacttc aactggtcgt gcgtgaagaa cgccaagctc gacggcctgc tcgccgacgg
     4381 ccggcgcgag gcggacccgg ccaagcggcg cgcgatctac ctggccgtcg agaagctcgc
     4441 gctcgacgag gcgctcacgg tgcccctggt ggacgagctg gcggtgtggg ccttccgggc
     4501 cccggtgcag ggcgtgaagt acaacttcaa cgcctacccg gtcctgagcg acgcgacgat
     4561 ccgccgctag ctccgccgcg tgctgaccta cgtcggccgg cggatcctcg ccgtggtccc
     4621 ggtcctgttc ggggtcacgc tggccgtctt cagcatgctc ttcctggtgc cgggcgaccc
     4681 ggtcaagatc atgctcgccg agttcgtcac cacgcccgac cagatcgccc agatgcgggc
     4741 ccagctccat ctcgacgagc cggtgctcaa gcagtacggc cgcttcgtga ccaacgcgct
     4801 gcgcggcgat ctcggggtct cgatccggag ccggcggccg gtggcggccg agatcaccga
     4861 gaacatcggg agcaccggcc agctcgcgct cgccagcatg ctggtcgcga tcgggctcgg
     4921 ggtgccgctc ggcctgctcg ccgcgctcgg gcgcaattcc tggctcgacg tggtcgcgat
     4981 ggtggtggcg ctgctcgggg tggccatgcc gagcttctgg ctcggcttcc tgctgatctt
     5041 cgtcttctcc ctgcacctcg gctggctccc cgccaccggg ggcggcgatc tgccccacct
     5101 cgtcatgccg gcggtggccc tcggcatgat cgcggccgcg atcatcgcgc ggctcacccg
     5161 ctcgagcatg ctcgaggtgc tgggccagga ctacgtgcgg accgcgcggg ccaagggcct
     5221 cgggagggcg agcgtgatcg tgcgccacgc gctccggaac gccctgatcc cggtggtgac
     5281 cgtcttcggc ctgcagttcg ggaacctgct ggccggcgcg gtgatcgtgg agaccgtctt
     5341 cagccgcccg gggctcggcc ggctcatcgt gggcggcatc ctggccaagg acttcccgct
     5401 ggtgcagggg acagtcctgt tcgtggccgc cgcgtacgtg ctgatcaacg tggtggtcga
     5461 cgtggcctac gcctacgtcg acccgcggat ccggatcgga tgagcgcggc ggccggcctg
     5521 ctgcggcacc gcggcgccct gatcggcctc gtgatcctgg gagcgctcgc gacgatggcg
     5581 gtggccgcgc cctggatcag cgcgcgcgac ccgatccgga ccgcgccccg ggaggccctg
     5641 caggcgccgg gggcgcgctt cgtgctcggg agcgatcagt tcgggcgcga cgtggcgagc
     5701 cgggtgctct acggggcccg cgtctcgctg ctggtcggcg tcatctcggt ctcgatcgcg
     5761 gtggggctcg gggccccgct cgggctggtg tcgggctact acggcggccg cctcgacgcg
     5821 ctgatcatgc gagtgatgga cgtgatgctc gcgttcccgg gcatcctgct cgcgctcgcc
     5881 atcgtgagcg tgctcagccc cgggctcggc aacgtgatga tcgcggtggg cctctccgcg
     5941 gtgcccggct acgcccgcct cgtccgggcc accgtgctct ccgcgcggga gcacctctac
     6001 gtggaggcgg cccgcgccct cggcggccgc gacgggggca tcctggtgcg cgacatcct
//
LOCUS       DATMCV010001125         2102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_331354, whole genome shotgun sequence.
ACCESSION   DATMCV010001125 DATMCV010000000
VERSION     DATMCV010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331354"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VNU03_25795"
     CDS             <1..1351
                     /locus_tag="VNU03_25795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWP77961.1"
                     /translation="VVVDWFITPPLYSISLDFDFGLRIAVFALSALLVGWLSERRRRT
                     EHALRLSRDELEARVQARTADLSRSNTRLEAEIGARVQVEETLREQAALLDLTHDTVF
                     VRDERDVIVYWNRGAEELYGWTKEEAVGTVAHTRMQTLFPTPLPDIMAELSRAGRWEG
                     ELVHTRRDGSPVTVASRWSLRRGAPGRAPAVLETNNDITERKRAEEALHQAQAELARV
                     TRVTTLGELAASIAHEINQPLAAIVADANACLNWLGADHPPLAQVREALGAIVSDGNR
                     AADVLIRIRSLLARSVVAREPCQLDRIVADVLPLVRPELGRQGIALQMSLAVDLPPVL
                     GDRIQLQQVLINVLVNAAEAMREVPLERRHLDVRAACELVDDRPCAVVAVEDRGVGLG
                     GLEPDRLFDAFYTTKPGGLGMGLSISRSIVEGHGGRLWATANPDQGATFHLALPGVG"
     gene            1468..1854
                     /locus_tag="VNU03_25800"
     CDS             1468..1854
                     /locus_tag="VNU03_25800"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HWP77962.1"
                     /translation="MDDIRSELQEKLKHTVDRLKGLGGAVAFEDDPGAREDEGQGEAA
                     GDAGRISEDREMTFAVRGLLVERANRLADALERLRSGDYGSCEACGEPIAPARLRAIP
                     EVTTCVTCQDAEERRARLAPEALTPR"
     gene            1919..>2102
                     /locus_tag="VNU03_25805"
     CDS             1919..>2102
                     /locus_tag="VNU03_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77963.1"
                     /translation="MAKWRVHPDEEPSRQVQALADQVERDGGTVLAVYQEPVGDHWQI
                     FALLPMDKVEPTPYQRD"
BASE COUNT          292 a          756 c          780 g          274 t
ORIGIN      
        1 ggtggtcgtc gactggttca tcactccccc gctctactcg atcagcctcg acttcgactt
       61 cggcctgcgc atcgcggtct tcgcgctgtc ggccctcctg gtcggctggc tgagcgagcg
      121 gcgccgccgc accgagcacg cgctgcggct ctcgcgcgac gagctggagg cgcgggttca
      181 ggcgcggacc gcggacctct cgcggagcaa cacccggctc gaggccgaga tcggggcgcg
      241 ggtgcaggtc gaggagacgc tccgggagca ggccgcgctc ctcgacctga cccacgacac
      301 cgtcttcgtg cgcgacgagc gcgacgtcat cgtctactgg aatcgcggcg cggaggagct
      361 gtacgggtgg acgaaggagg aggcggtcgg cacggtcgcc cacacccgca tgcagaccct
      421 cttcccgacg ccgctgccgg acatcatggc cgagctatcc cgcgccgggc ggtgggaggg
      481 cgagctggtc cacacgaggc gcgacgggtc gccggtgacg gtggcgagtc gctggtccct
      541 gcggcgcggc gcgcccggcc gggcccccgc ggtgctcgag acgaacaacg acatcaccga
      601 gcgcaagcgc gccgaggagg ccctgcacca ggcccaggcc gagctggcgc gggtgacgcg
      661 cgtgacgact ttgggcgagc tggccgcgtc gatcgcccac gagatcaatc agcccctggc
      721 cgcgatcgtc gccgacgcca acgcctgcct caactggctc ggcgccgacc atccgccgct
      781 ggcccaggtc cgcgaggcgc tcggcgccat cgtgagcgac gggaaccggg ccgcggacgt
      841 gctgatccgg atccgctccc tgctcgcgcg ctcggtcgtg gcccgcgagc cctgccagct
      901 cgaccggatc gtcgccgacg tgctcccgct cgtgcgtcct gagctcggcc gccaggggat
      961 cgcgctccag atgtcgctcg cggtggacct gccaccggtc ctgggagatc ggatccagct
     1021 ccagcaggtg ctcatcaatg tcctcgtcaa cgcggccgag gcgatgcgag aggtcccgct
     1081 cgagcggcgc cacctcgacg tccgggccgc ctgcgagctc gtcgacgatc gcccctgcgc
     1141 ggtcgtcgcg gtggaggacc gcggcgtcgg cctcggcggg ctcgagcccg accggctctt
     1201 cgacgcgttc tacaccacga agccgggcgg gctcggcatg gggctgtcga tcagccggtc
     1261 gatcgtcgag gggcacggcg gccgcctctg ggccaccgcg aacccggacc aaggcgccac
     1321 ctttcacctg gcgctgcctg gagtcggctg aggaccagcc cttgagaggg ccgggcggcg
     1381 gattccggcc gggcccgatc gctccgcgga tccattcggg gtccggccgc atccaaagac
     1441 gcgattcggc cggaggaggg ccgacccatg gacgacatca gaagcgaact acaggaaaag
     1501 ctgaagcata cggtcgatcg cctgaagggg ctgggtgggg cggtggcctt cgaggatgat
     1561 ccgggggccc gggaggacga gggtcagggc gaggccgccg gcgacgcggg gcggatcagc
     1621 gaggaccgcg agatgacgtt cgcggtgcgg ggtctcctcg tcgagcgggc caatcgcctc
     1681 gccgacgcgc tggagcgcct ccggtccggc gactacggga gctgcgaggc ctgcggcgag
     1741 ccgatcgccc cggcccgcct ccgggccatc cccgaggtca cgacctgcgt cacgtgccag
     1801 gacgcggagg agcgccgcgc ccgcctggcg ccggaggcgc tgacgccccg ctgaccccgc
     1861 cgcccgcgtg ccggccgctt ccttgcgccg gcgcgcgggc ggcatgataa tcgtccgcat
     1921 ggcgaagtgg cgggtccacc ccgacgagga gccgagccgc caggtgcagg cgctggcgga
     1981 ccaggtcgag cgcgacggcg gcacggtgct cgcggtctac caggagccgg tcggcgatca
     2041 ctggcagatc ttcgcgctgc tcccgatgga caaggtggag ccgaccccgt accagcgcga
     2101 tc
//
LOCUS       DATMCV010001126         5963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_264243, whole genome shotgun sequence.
ACCESSION   DATMCV010001126 DATMCV010000000
VERSION     DATMCV010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264243"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VNU03_25810"
     CDS             <1..1447
                     /locus_tag="VNU03_25810"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWP77964.1"
                     /translation="REQFALSMALVLTLGILPPAVQAQTPPPATTLPPAAPPPAVMQT
                     PPSMPPREPLPAPPGIRPFATPQELVGRELGLEEAVNIGLENAPLIVARIGDYIAAQQ
                     RVNQALAPLLPQLTGSGNFGRQRSLSTFGGDAGYNDFGSASVTASQLLFDFGRTWAAK
                     DAAKSNAAAFKEVLESQKLDISQLVKTQYFTLLLSKRLVQVNLAALDRAEVNLRSAQG
                     FFQVGTQPKSFVTRAEVDVANARVNVIRAQNAVNLARVALNAAMGIAVNAPTEVKDLL
                     SYSQFPINRDELIAEALRNRPEYRQVKALADSADATVRQTFRDFFPNLFGSGTYGVAG
                     ITGFPAADSRSTNGFIDSGNQWNVGLTLSWSIFDGGGKIARYKESKANLEAAKARVRD
                     TELQVWQNVEQSYLNLGEAEERIGAAQKAVESAEENYQLARGRFDAGVANIIELTDAQ
                     LALTQAQSDQAQALSDYRIGIARLERALGRR"
     gene            1481..2878
                     /locus_tag="VNU03_25815"
     CDS             1481..2878
                     /locus_tag="VNU03_25815"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWP77965.1"
                     /translation="MKRRILWIVAIVVFAGAMTGGYLYTQGVGARPAFRTAAVSRGPL
                     TSAVSATGTLNAVITVQVGSQVSGQIKELHADFNSLVKKDQVIARIDPDTFEAKVNQT
                     KAQVEAARASVLNQQAVVEKTRADLENTRAAFAAARAQTAKSQVAVVDTNRTLGRNRD
                     LRLRGLIAQADEDTAQATYDSAVAQLEASKAQELAQVSTVRAAEAQLRVTEAMLKNAV
                     AQVAQNEAAQRQAQVDLDHTYIRAPVDGVVVGRTVDVGQTVAASLQAPTLFTIAQDLR
                     NMQVDTNVDEADVGRVKVGQATSFTVDSFPGRTFTGEVVQIRKAPLVVQNVVTYDVVV
                     SAKNAEQRLLPGMTANVRIVIDQKEGVLQVPNAALRFRPAGVEATERERPARTGGPGG
                     GGGRGGGAPAGRVWVLGPDGKPAALALQLGIGDGTYTEVARGELKEGQLVIVGVAVPG
                     DRTPAQTGAAPRLRF"
     gene            2880..3659
                     /locus_tag="VNU03_25820"
     CDS             2880..3659
                     /locus_tag="VNU03_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWP77966.1"
                     /translation="MADSLIETVDLVKDYHLGSRRIHALRGVSMTIAPGEFVAVMGPS
                     GSGKSTFMNLLGCLDTPSAGLYRLDGLDVSRMGRDALAHVRNEKIGFVFQTFNLLPRT
                     SALENVELPLLYSAALPRDRQARARAKLAAVGLSEREHHHPAQLSGGQQQRVAIARAL
                     INNPVLILADEPTGALDSRTSVEIMALFQDLNREGITVVVVTHEADIARYAGRTLSFR
                     DGHLTHDEPVSDRLVARELLATLPSGEVSDEEDVETEVSPT"
     gene            3656..4888
                     /locus_tag="VNU03_25825"
     CDS             3656..4888
                     /locus_tag="VNU03_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP77967.1"
                     /translation="MNVLASARIAVRALRVNKMRSALTMLGIVIGVGAVITMVAVGAG
                     AQARVAEQIQSLGANIIVIWPGTVNVSGVRLGAGAQATLSEDDAWAVQRDIPLVDVVA
                     PYVRGNAQVVYSNLNWWTGILGITPEYFPARDWEVIEGRMLTQEEVDGASKVALVGQT
                     VARNLFGDEDPLGQVIRIKKVPFTIIGVLDRKGQTTWGQDQDDQILIPITTARTKVLG
                     GNQAKGRAVGSITIKVLDADLMKAAENEIRELLRQRHRLQPHQEDDFNLRNLAEVLQS
                     QEAASRVLTILLAAIASVSLLVGGIGIMNIMLVSVTERTREIGLRMAVGARGRDILAQ
                     FLVEAVTLSLIGGVIGIGAGLLGSYAIAYFAEWRTLLQVEAVLIAFGFAAAVGIFFGF
                     YPARKAAGLNPIDALRYE"
     gene            4885..5262
                     /locus_tag="VNU03_25830"
     CDS             4885..5262
                     /locus_tag="VNU03_25830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77968.1"
                     /translation="MIARRLLGAALAWVTLCATVEAQTYSPQSPSRLAVSFTTERVGS
                     GRVLVYGEVRNGASSACERVIVGVEGLDENGRVVSRGRAFVFGVVPSRGSSTFEVRMS
                     SPGTEKRFRVEIESFQFASSSGN"
     gene            5277..5858
                     /locus_tag="VNU03_25835"
     CDS             5277..5858
                     /locus_tag="VNU03_25835"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWP77969.1"
                     /translation="MVKKRKKPASSRRRRARRGRARRARAIVPAPPAPAPPPPAAPAG
                     PGPTQVEQRGLPIVTALARIARTGDPPRARLELAIRLLLAAYGESDPDFSGLFLAGWA
                     RARHDKQFRLTLAWQREQIRLSLEEILVEGARVGGFRANLDAGAVAAVIVGAAEGCLL
                     QAVTEGGAVPAERLVHTLLTLALSGPDARARRA"
BASE COUNT          868 a         2174 c         2108 g          813 t
ORIGIN      
        1 gcgggagcaa tttgcgctgt ccatggcgct ggtcctcacc ctggggatac tgccgcccgc
       61 ggtccaggcg cagacacccc ctccggcgac cacgctgccg ccggccgccc cgccgccggc
      121 ggtgatgcag acgcccccgt cgatgccgcc gcgcgagccc ctgccggccc cgcccggaat
      181 ccggccgttc gccacgcccc aggagctggt cgggcgcgag ctcggactgg aggaggcggt
      241 caacatcggg ctcgagaacg cgccgctgat cgtggcgcgc atcggcgact acatcgcggc
      301 ccagcagcgc gtgaaccagg ccttggcccc gctcctcccg cagctcaccg gctccggcaa
      361 cttcggccgg cagcgctcgc tcagcacctt cggcggcgac gccgggtaca acgacttcgg
      421 ctcggcgagc gtcaccgcct cccagctcct gttcgacttc ggaaggacct gggccgccaa
      481 ggacgcggcc aagtccaacg cggccgcgtt caaggaggtg ctcgagtcgc agaagctcga
      541 catctcccag ctcgtgaaga cgcagtactt cacgctgctc ctctccaagc ggctcgtcca
      601 ggtgaacctg gccgcgctcg accgcgccga ggtcaacctg cggagcgccc agggcttctt
      661 ccaggtgggg acccagccca agagcttcgt cacgcgggcc gaggtggacg tggcgaacgc
      721 gcgcgtcaac gtcatccggg cccagaacgc ggtgaacctg gcccgggtgg ccctgaacgc
      781 ggccatgggc atcgcggtca acgcgccgac cgaggtcaag gacctgctct cctactcgca
      841 gttcccgatc aaccgcgacg agctcatcgc ggaggcgctc cggaaccggc ccgagtaccg
      901 gcaggtgaag gcgctcgccg attccgccga cgcgaccgtc cgtcagacct tccgggactt
      961 cttccccaat ctgttcggca gcggcaccta cggggtggcg ggcatcaccg ggttccccgc
     1021 ggccgactcg cggtcgacga acggcttcat cgactccggc aaccagtgga acgtcgggct
     1081 gaccctgagc tggtcgatct tcgacggcgg cggcaagatc gcccggtaca aggaatccaa
     1141 ggcgaacctg gaggccgcga aggcgcgggt gcgcgacacc gagctgcagg tctggcagaa
     1201 cgtcgagcag tcctacctca acctcggaga ggccgaggag cggatcgggg ccgcgcagaa
     1261 ggcggtggag tccgccgagg aaaactacca gctggcccgg gggcgcttcg acgcgggcgt
     1321 ggccaacatc atcgagctga ccgacgccca gctcgccctc acccaggccc agtccgacca
     1381 ggcccaggcc ctctccgact accggatcgg gatcgcgcgg ctggagcggg cgctcggccg
     1441 gcgctaggaa taccgccgct cgcccccgcg tcaccccatc atgaagcgcc gaatcctctg
     1501 gatcgtagcg atcgtggtgt tcgcgggggc gatgaccggg ggctatctct atacccaggg
     1561 cgtcggggcc cgccccgcct tccgcaccgc cgcggtctcg cggggtccgc tcacctccgc
     1621 ggtgtcggcc accggcaccc tcaacgcggt gatcacggtg caggtgggct cccaggtgtc
     1681 cgggcagatc aaggagctcc acgccgactt caactcgctg gtgaagaagg accaggtcat
     1741 cgcccgcatc gatcccgata ccttcgaggc caaggtcaac cagacgaagg cccaggtgga
     1801 ggcggcgcgc gcgtcggtgc tgaaccagca ggcggtggtg gagaagaccc gggcggatct
     1861 cgagaacacc cgcgccgctt tcgcggccgc gcgcgcgcag accgcgaagt cccaggtcgc
     1921 ggtggtcgac accaatcgca cgctcggccg caaccgggac ctgcgcctgc gggggctcat
     1981 cgcgcaggcc gacgaggaca ccgcgcaggc cacctacgac tccgcggtcg cccagctcga
     2041 ggcctccaag gcccaggaac tggcgcaggt gtcgacggtg cgcgccgcgg aggcccagct
     2101 gcgggtgacc gaggcgatgc tgaagaacgc ggtcgcgcag gtggcccaga acgaggcggc
     2161 ccagcgccag gcccaggtgg atctggacca cacctacatc cgcgcgccgg tcgacggcgt
     2221 ggtggtgggc cgcaccgtcg acgtcggcca gacggtggcg gcgagcctcc aggccccgac
     2281 cctgttcacg atcgcccagg acctgcgcaa catgcaggtc gacaccaacg tcgacgaggc
     2341 cgacgtgggg cgggtcaagg tgggccaggc gaccagcttc acggtcgact ccttcccggg
     2401 ccggaccttc accggagagg tcgtgcagat ccgcaaggcg cccctggtgg tgcagaacgt
     2461 tgtcacctac gacgtggtgg tgagcgccaa gaatgcggag cagcgcttgc tgcccggcat
     2521 gaccgccaac gtgcgcatcg tcatcgacca gaaggagggc gtgctgcagg tgccgaacgc
     2581 ggcgctgcgc ttccgcccgg ccggcgtcga ggccaccgag cgcgagcggc ccgcgcggac
     2641 cggagggccc ggcggcggag gcggccgtgg cggcggagcg cccgccgggc gggtgtgggt
     2701 cctcggcccc gacggcaagc ccgccgcgct cgccctgcag ctcggcatcg gcgacgggac
     2761 ctacaccgag gtggcccgcg gcgagctcaa ggaaggccag ctggtgatcg tgggcgtcgc
     2821 ggtgccgggc gaccggaccc cggcgcagac cggcgccgcc ccgcgccttc ggttctgaga
     2881 tggccgactc tctcatcgag acggtggacc tggtcaagga ctaccacctg ggctcccggc
     2941 gcatccacgc cctccggggc gtctcgatga cgatcgcccc gggcgagttc gtggcggtga
     3001 tgggcccgtc cggctccggc aagtcgacct tcatgaacct gctcggctgc ctcgacaccc
     3061 cgagcgcggg gctctaccgg ctcgacgggc tcgacgtctc gcgcatgggc cgcgacgcgc
     3121 tggcccacgt gcgcaacgag aagatcggct tcgtgttcca gaccttcaac ctgctgccgc
     3181 gcaccagcgc gctcgagaac gtggagctgc cgctgctcta cagcgcggcc ctcccgcgcg
     3241 atcggcaggc gcgcgcgcgc gccaagctgg ccgcggtcgg gctgtcggag cgggagcacc
     3301 accacccggc ccagctctcg ggcggccagc agcagcgggt ggcgatcgcg cgggccctca
     3361 tcaacaaccc ggtgctcatc ctggccgacg agccgaccgg ggcgctcgac tcgcggacga
     3421 gcgtggagat catggccctc ttccaggacc tcaaccgcga gggcatcacc gtcgtggtgg
     3481 tgacccacga ggccgacatc gcgcgctacg cggggcgcac cctcagcttt cgcgacggcc
     3541 acctcaccca cgacgagccg gtgtccgatc gcctggtcgc ccgcgagctg ctcgccaccc
     3601 tgccgagcgg agaggtctcc gacgaggaag acgtggagac ggaggtctcg cccacgtgaa
     3661 cgtcctggcc agcgcgcgga tcgcggtgcg ggccctgcgc gtcaacaaga tgcgcagcgc
     3721 gctcaccatg ctcggcatcg tgatcggggt gggcgcggtc atcaccatgg tggcggtggg
     3781 cgcgggggcc caggcccggg tggccgagca gatccagagc ctcggggcca acatcatcgt
     3841 gatctggccc gggacggtga acgtctccgg ggtgcggctc ggcgcggggg cccaggcgac
     3901 cctctcggag gacgacgcgt gggccgtcca gcgcgacatc ccgctcgtcg acgtggtggc
     3961 gccgtacgtg cggggcaacg cgcaggtggt ctactcgaat ctcaactggt ggaccggcat
     4021 cctcgggatc acccccgagt actttccggc gcgcgactgg gaagtcatcg aaggccggat
     4081 gctcacccag gaagaggtgg acggagcgtc gaaggtcgcg ctggtgggcc agaccgtcgc
     4141 ccgcaacctg ttcggcgacg aggatccgct cggtcaggtg atccgcatca agaaggtgcc
     4201 cttcaccatc atcggggtgc tggaccgcaa gggccagacc acgtggggcc aggaccagga
     4261 cgaccagatc ctgatcccca tcaccaccgc caggaccaag gtgctgggcg gcaaccaggc
     4321 caagggccgc gcggtgggct cgatcaccat caaggtgctc gatgccgatt tgatgaaggc
     4381 ggccgagaac gagatccggg agctgctgcg tcagcgccac cgcctgcagc cgcaccagga
     4441 ggacgacttc aatctccgga atctggccga ggtgctccag tcccaggagg ccgcctcccg
     4501 ggtgctcacc atcctgctcg cggccatcgc gtcggtctcg ctgctggtgg gcggcatcgg
     4561 tatcatgaac atcatgctgg tctcggtgac ggagcgcacc cgggagatcg gcctgcgcat
     4621 ggcggtgggt gcgcggggcc gcgacatcct ggcccagttc ctggtggagg cggtcaccct
     4681 gtcgctcatc ggcggcgtca tcggcatcgg cgccggcctc ctcggctcct acgccatcgc
     4741 ctacttcgcg gagtggcgta ccctgctcca ggtcgaggcg gtcctgatcg ccttcggctt
     4801 cgcggccgcg gtgggcatct tcttcggctt ctacccggcg cgcaaggcgg ccgggctcaa
     4861 ccccatcgac gccctccggt acgaatgatc gcccgccggc tgctcggtgc cgcgctcgcc
     4921 tgggtcacgc tctgcgcgac ggtcgaggcc cagacctact ccccgcagtc cccctcccgc
     4981 ctcgcggtga gcttcaccac cgagcgcgtg ggcagcggcc gggtgctcgt gtacggggag
     5041 gtgcgcaacg gggccagctc cgcgtgcgag cgggtgatcg tgggcgtcga ggggctcgac
     5101 gagaacggcc gcgtggtcag ccggggccgg gccttcgtct tcggggtcgt cccctcccgg
     5161 ggctcgagca ctttcgaggt gcgcatgtcc tctcccggga ccgagaagcg cttccgcgtg
     5221 gagatcgagt ccttccagtt cgcgtcctcc tcggggaact gacgcccgcg gtcgtggtgg
     5281 tgaagaagcg aaagaagccc gcctcatccc gccgccggcg cgcgcgccgc ggccgggcgc
     5341 gccgcgcccg cgcgatcgtc ccggcgccgc cggccccggc cccgccgccg cccgcggctc
     5401 cggcgggacc cggcccgacc caggtcgagc agcgcgggct ccccatcgtc accgcgctgg
     5461 cgcggatcgc gcggaccggc gatcccccgc gcgcgcgcct cgagctggcc atccgcctgc
     5521 tcctggccgc ctacggcgag agcgaccccg acttctccgg gctcttcctg gccggctggg
     5581 cccgggcccg gcacgacaag cagtttcgcc tgaccctggc ctggcagcgc gagcagatcc
     5641 ggctctccct cgaggagatc ctggtggagg gcgcgcgggt cggcggcttc cgcgcgaatc
     5701 tggacgcggg cgcggtggcc gcggtcatcg tgggagccgc cgagggctgc ctcctgcagg
     5761 cggtcaccga gggcggcgcg gtccccgccg agcgcctggt gcacaccctc ctgacgctcg
     5821 cgctcagcgg gcctgacgct cgcgctcggc gggcctgacg ctcgcgctcg gcgggcctga
     5881 cgctcgcgct cggcgggcct gacgctcgcg ctcggcgggc ctgacgctcg ccctcagcgg
     5941 ggcttgagct cgatgcggta gcc
//
LOCUS       DATMCV010001127         5162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_62275, whole genome shotgun sequence.
ACCESSION   DATMCV010001127 DATMCV010000000
VERSION     DATMCV010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62275"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VNU03_25840"
     CDS             <1..1212
                     /locus_tag="VNU03_25840"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWP77970.1"
                     /translation="WRGPLHGVPLAFKDLCFIRGLPTSCGTRTAEYFTADQDCTAVSR
                     LLAAGAVCLGKLNMTELAMGALGDNPHHGHARNPWAVEHCTGGSSSGSGVAVAAGLAP
                     GAIGTDTGGSIRLPAASCGITGLKPTYGRVSRAGAMPLSWSNDHLGPMARTALDCALM
                     LQAMAGADPLDATTSSLPVADYVASLEIPVRGLRVGVPLDFFFQGLDAEMEAGVRSAV
                     ATLATLGASVAEIRLPDPRVLNDITGLVSRSESSALHGRLLRERPQELDAFTRARLEL
                     GLGVPAYDYLQALRLRARLARAFTREVWSEVDLLAVPVVPEPAPPLRVALEGPLEERI
                     ARQGRFSRLTRPFNGLGLPALSLPCGFSAAGLPLAFQLVGRPFDEATVLRVGHTYQRA
                     TDWHTREPDLG"
     gene            complement(1215..1856)
                     /locus_tag="VNU03_25845"
     CDS             complement(1215..1856)
                     /locus_tag="VNU03_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008233324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HWP77971.1"
                     /translation="MDQISIYLPGILLAYATFFLAIASPGPNVLAIIGTSMSVGRRSG
                     IALALGVAAGSFVWAMLAAVGLTALLASYAAALTAIKIAGGLYLLWLSYKSFRSAASP
                     HDIEATTLSGSTRTPFGYAVRGFAIQMTNPKAALAWIAIISLGLREHAPLWVAFAIVV
                     GTSILSVIIHWLYAVAFSTPVMVRAYSRARRWIQGALGAVFAVAGIKLLTSRT"
     gene            complement(1864..2547)
                     /locus_tag="VNU03_25850"
     CDS             complement(1864..2547)
                     /locus_tag="VNU03_25850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77972.1"
                     /translation="MTMAGEGVLAFWHDVAPGGDGEFDQWHLREHIPERLAVPGFLRA
                     RRYATLGAPPRYFYFYETESLDTLQSPAYLARLADPTPWTRRTMPLVRNNIRTACRVV
                     ASWGAAVGGVIATLQLGPRAGHEDELRSWLSTTALPGAAEHPGIVASHLLEGDAGVST
                     AAKSDEKKLLATPDALVRWVVLVEGIDAAATEATCRDLLSPETLARHGGADGTTLGLY
                     RLQLVLGER"
     gene            2621..4096
                     /locus_tag="VNU03_25855"
     CDS             2621..4096
                     /locus_tag="VNU03_25855"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWP77973.1"
                     /translation="MAEPTETAPAAPPAPRRLVRGLLGGVRLAYLPVLVTYFCYGASG
                     VTAIALLYFQKDVLGITPAEAAGIAFWVALPWSTKMVAGVASDVHPILGSRRFAYLLL
                     GALLTLVGYAWLATTAATKGSYLAALVISAVGFMVQDVVADALSVEVARSEEEIKQIQ
                     ALGRMSLLAGTISVGYLSGWLAGAVGARTVFAIALILPLLVVVAAWLIPREPARPAAS
                     ADAPNPLGAGNSRLVVAGGLAYAALGILLEALSVPFGQEIILVVSGTIITLLLRRVGI
                     SRAVAVAAFAIFCFRAVPGSGQGFNYWAIDHLGFDQEFLGLLAQVSSMLSLVGLIVFR
                     KPITHRPVSYTLFWVTIAGAVLYLPTIGLFYEAYRWFGLSPRTFAFIDTTISAPLGQL
                     SMVPMLALIARTAPRGAEATMFAIMASLMNLALSASELGTRYLNVAFGVTQQDYSGLG
                     QLLIAVGLIGLLPLLALPLLRREEATSLAAAPPAAAPARPA"
     gene            4131..>5162
                     /locus_tag="VNU03_25860"
     CDS             4131..>5162
                     /locus_tag="VNU03_25860"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWP77974.1"
                     /translation="MALTAPTRTRWAAPLRVFERDERLFAVLLAIVMVGGLTALGLVP
                     LRPRHRVDVYALVIWFGLYKAGILALVTVNPGATRRIFTAALCVDLALVFLLLYLTGG
                     GDSPFVNLFFPLVAVNAYYFGRWVGLLLTLVAGMLYWTAAWMAPPDVAWTAVAIVMGQ
                     LGLPAFALGHVADRERRARVEVERLNAEVTGTLTRLQAAQENLVEAERMATVGRLSLK
                     VAHEVRNPIAAIELNAELLGDMVQGRVSEDGTEATALVGAIRDQVAALDALTEEYLAF
                     ARFPRPQFEEDSVNDMVSAVADFVRPLAARQGIAVKVATDAAVPPMEFDRTLLRQAVL
                     NLLKNGIEAV"
BASE COUNT          648 a         1870 c         1869 g          775 t
ORIGIN      
        1 tggcggggcc cgctccacgg cgtgccgctc gcattcaagg atctctgctt catccgcggc
       61 ctgcccacct cctgtggcac ccggaccgcg gagtacttca ccgccgacca ggactgcacc
      121 gcggtgagcc ggctcctcgc cgccggcgcg gtgtgcctcg gcaagctcaa catgaccgag
      181 ctggccatgg gcgcgctggg cgacaacccg catcacgggc acgcgcgcaa tccgtgggcg
      241 gtcgagcact gcaccggggg ctcgtcgagc ggctcgggag tggcggtggc ggcggggctc
      301 gcgccgggcg ccatcggcac cgacaccggc ggctccatcc gcctgcccgc cgcctcctgc
      361 ggcatcaccg ggctcaagcc gacctacggc cgggtgagcc gcgcgggcgc gatgccgctc
      421 tcgtggtcga acgaccacct gggtccgatg gcccgcaccg cgctcgactg cgcgctcatg
      481 ctccaggcca tggcgggcgc cgatccgctc gacgcgacca cgagctcact cccggtcgcc
      541 gactacgtcg cgtccctcga gatcccggtg cgcgggctcc gcgtcggcgt gccgctcgat
      601 ttcttcttcc agggcctcga cgccgagatg gaggcggggg tgcgtagcgc ggtcgcgacc
      661 ctggccaccc tgggggccag cgtcgcggag atccggctgc ccgatccccg ggtgctcaac
      721 gacatcaccg ggctcgtctc ccgcagcgag tcgagcgcgc tgcacggccg cctgctccgg
      781 gagcggccgc aggagctgga cgccttcacg cgcgcccgcc tcgagctggg cctgggcgtg
      841 ccggcctacg attacctgca ggccctgcgg ctacgcgcgc gcctggcccg cgcgttcacc
      901 cgcgaggtgt ggagcgaggt cgacctgctg gcggtgccgg tggtcccgga gccggcgccg
      961 cccctccgcg tggcgctcga gggcccgctg gaggagcgca tcgcgcggca gggccggttc
     1021 tcgcgcctca cgcgcccgtt caacgggctc ggcctgcccg cgctgagcct gccctgcggc
     1081 ttctccgccg ccggcctgcc gctcgcgttc cagctggtgg gccgtccgtt cgacgaggcc
     1141 accgtcctgc gcgtgggcca cacctatcag cgcgcgaccg actggcacac gcgcgagccc
     1201 gacctcggct gagctcaggt gcggctggtc agcagcttga tgcccgccac cgcgaacacc
     1261 gcgccgagcg ccccctggat ccaccgccgc gcccgggagt acgcgcgcac catgaccggg
     1321 gtggagaacg cgaccgcgta gagccaatgg atgatgaccg acaggatcga cgtaccgacc
     1381 acgatggcga acgcgaccca cagcggcgcg tgctcccgga gcccgagcga gatgatcgcg
     1441 atccaggcga gcgccgcctt gggattggtc atctggatgg cgaacccgcg caccgcgtag
     1501 ccgaaggggg tgcgggtcga tccggagagc gtggtggcct cgatgtcgtg aggcgaggcg
     1561 gcggagcgga acgacttgta ggacagccag agcaggtaga gtccgcccgc gatcttgatg
     1621 gcggtgaggg cggccgcgta cgatgccagc agcgcggtca ggccgaccgc cgcgagcatg
     1681 gcccagacga aggagcccgc cgccactccg agtgcgagcg cgatgcccga ccgccgcccc
     1741 acgctcatcg acgtgccgat gatcgccagc acgttcgggc cggggctggc gatcgcgaga
     1801 aagaacgtcg cgtacgcgag gaggatgccg ggaaggtaga tcgagatctg gtccatgtcg
     1861 gcgtcacctt tctcccagta cgagctgcag gcggtacagc ccgagggtcg tgccgtccgc
     1921 gcccccgtgt cgggcaaggg tctcggggct gagcaggtcg cggcacgtcg cctccgtggc
     1981 ggccgcgtcg atgccctcga cgagcaccac ccagcgcacg agcgcgtcgg gagtcgcgag
     2041 gagtttcttc tcgtcgctct tggccgcggt gctgacgccc gcgtcgccct cgaggaggtg
     2101 gctcgccacg atcccggggt gctccgccgc cccggggagc gcggtggtgg agagccagga
     2161 ccgcagctcg tcctcgtggc ccgcgcgggg gccgagctgg agcgtggcga tcactccgcc
     2221 caccgccgca ccccacgagg ccacgacgcg gcacgcggtg cggatgttgt tgcgcaccag
     2281 cggcatggtc cgccgcgtcc acggggtcgg atcggcgagg cgggccaggt aggccgggct
     2341 ctgcagcgtg tcgagcgact cggtctcgta gaagtagaag taccggggcg gggcgcccag
     2401 cgtggcgtag cgtcgcgcgc ggaggaagcc gggcaccgcg aggcgctccg ggatgtgctc
     2461 gcgcaggtgc cactgatcga actcgccgtc cccgccgggc gccacgtcgt gccagaacgc
     2521 gagcacgccc tcgccggcca tcgtcatgcc gccgacggta acggcggcgc gggccgacgt
     2581 caagcgtgag acggcggccg ggtacgctac actgcgtctg atggccgaac cgaccgagac
     2641 ggctcccgcc gcgccgcccg cgccccgccg tctcgtgcgc ggcctgctgg gcggggtgcg
     2701 gctggcctat ctcccggtgc tggtgaccta cttctgctac ggggccagcg gggtcaccgc
     2761 gatcgcgctg ctctattttc agaaggacgt gctcggcatc acccccgccg aggcggccgg
     2821 catcgccttc tgggtggcgc tgccgtggag caccaagatg gtggcggggg tcgcgtccga
     2881 cgtgcatccg atcctcggga gccgccgctt cgcgtatctg ctgctgggcg cgctgctgac
     2941 gctggtgggc tacgcgtggc tcgccaccac cgccgccacc aagggcagct acctggctgc
     3001 cctcgtcatc agcgcggtgg gcttcatggt ccaggacgtg gtggccgacg ccctgagcgt
     3061 ggaggtggcc cgctccgagg aggagatcaa gcagatccag gccctgggcc gcatgtcatt
     3121 gctggccggc accatcagcg tgggctacct gagcggctgg ctcgccggcg cggtcggggc
     3181 gcggacggtg ttcgcgatcg cgctgatcct gccgctcctg gtggtggtgg cggcctggct
     3241 gatcccgcgg gaaccggcgc ggcccgccgc ctccgccgat gcgcccaatc ccctcggggc
     3301 gggcaactcg cgcctcgtgg tggcgggcgg gctcgcgtac gccgcgctcg ggatcctgct
     3361 ggaggcgctg tcggtgccgt tcgggcagga gatcatcctg gtcgtctccg gcacgatcat
     3421 cacgctgctc ctccggcggg tcgggatctc ccgcgccgtc gcggtcgccg ccttcgcgat
     3481 cttctgcttc cgggcggttc ccgggtcggg gcaggggttc aactactggg cgatcgacca
     3541 cctggggttc gatcaggaat tcctggggct cctcgcccag gtgagctcga tgctgagcct
     3601 ggtcggcctg atcgtgttcc gcaagccgat cacccatcgg ccggtgagct acaccctctt
     3661 ctgggtcacg atcgcgggtg cggtcctcta cctgccgacg atcggcctct tctacgaggc
     3721 ctaccggtgg ttcgggctga gcccgcgcac cttcgcgttc atcgatacca cgatctcggc
     3781 gccgctcggc cagctcagca tggtgccgat gctcgccctc atcgcgcgca ccgcgccgcg
     3841 cggcgcggag gccaccatgt tcgcgatcat ggcctcgctc atgaacctgg ccctgtcggc
     3901 gagcgagctc ggcacgcgct acctcaacgt ggccttcggg gtcacccagc aggactactc
     3961 ggggctggga cagctgctca tcgcggtggg cctgatcggg ctcctcccgc tgctggccct
     4021 gccgctcctc cggcgcgagg aagcgacgtc gctcgccgcc gccccgcccg ccgcggcgcc
     4081 ggcccgaccg gcttgaccct ccaaggccgc atgatagtgt gtcccacgtc atggcgctga
     4141 ccgcccccac ccgcacgcgc tgggccgcgc ccctccgggt gttcgagcgc gacgagcgcc
     4201 tgttcgcggt gctcctcgcc atcgtgatgg tgggcggcct gaccgccctc gggctggtgc
     4261 cgctccgtcc ccgccaccgg gtcgacgtct acgcgctggt catctggttc gggctctaca
     4321 aggccggcat cctcgcgctg gtgacggtga accccggcgc cacgcgccgg atcttcaccg
     4381 ccgcgctgtg cgtcgacctc gcgctggtgt tcctgctgct ctacctcacc ggcgggggcg
     4441 acagcccgtt cgtgaacctg ttcttcccgc tcgtcgcggt caacgcctac tacttcgggc
     4501 gctgggtcgg gctgctgctg accctcgtgg ccgggatgct ctactggacc gcggcgtgga
     4561 tggccccgcc ggacgtggcg tggaccgcgg tcgccatcgt catggggcag ctcgggctgc
     4621 ccgccttcgc gctcggccac gtggcggatc gcgagcggcg ggcccgcgtc gaggtggagc
     4681 gattgaacgc ggaggtgacc ggcacgctca cgcgcctgca ggcggcccag gagaacctgg
     4741 tggaggcgga gcggatggcg acggtgggcc ggctctcgct caaggtcgcc cacgaggtgc
     4801 gcaaccccat cgcggccatc gagctgaacg cggagctgct gggcgacatg gtgcaggggc
     4861 gggtgagcga ggacgggacg gaggccaccg cgctggtcgg ggccatccgc gaccaggtcg
     4921 cggccctcga cgcgctcacc gaggagtacc tcgccttcgc gcgcttccca cggccgcagt
     4981 tcgaggagga ctcggtcaac gacatggtca gcgcggtggc ggacttcgtg cgcccgctgg
     5041 cggcccgcca gggcatcgcg gtgaaggtgg ccaccgatgc cgcggtgcct ccgatggagt
     5101 tcgatcgcac gctgctgcgc caggccgtcc tgaacctact gaagaacggg atcgaggcgg
     5161 ta
//
LOCUS       DATMCV010001128         3576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_62384, whole genome shotgun sequence.
ACCESSION   DATMCV010001128 DATMCV010000000
VERSION     DATMCV010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62384"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VNU03_25865"
     CDS             <1..600
                     /locus_tag="VNU03_25865"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77975.1"
                     /translation="LAHPGGNVTGLSDAHADLVPKRLELLKQVVPSVTRVGILWSPAN
                     PGTAPQVRIAEGAAPALGLTVLPVAVKGPRRDDLDRAFATIERERLGSLFVIGDSTLG
                     VQRDRLAELSLKHRLPTSASHSRWAEGGLLMSYGTDFVDLFRRSASLVDRILKGAKPA
                     DLPVEQPTKFELVINMRTAKALGLTISPSLLSRADRVIE"
     gene            666..1937
                     /locus_tag="VNU03_25870"
     CDS             666..1937
                     /locus_tag="VNU03_25870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HWP77976.1"
                     /translation="MRIEVICTGDEVLTGKIVNTNFSYITQKLEDVGLSVDWETTVGD
                     DRENLLRAFHLAGERADAVIVNGGLGPTVDDLSQEIAAKAAGVELALNEDWLTRMEDF
                     FQRRSRVMPPNNRKQAMLPTTAEVIDNPIGTACGFAVDIGRARFYFTPGVPRELRRML
                     EEQIVPRLLAKAGLPGAIFLKRFHSYGLGESHVDSLLTGVEALVPDGSVKLGFRAHYP
                     QLETKLTARGRDMDDIRRKLEPIEREVRKRIGNFILAEDDQTLESVLLRDLRAQGATL
                     SLVETFTSGQMAARLAHLPGAETIFRRGTIARSLPEVWAAVGLAGAPPEGPLTPQVAE
                     TVARAARAQTGATHALAVLIDLDDGPDRIEFGGTICLAIATASDAASRKARILGGREW
                     VRLGAVEMGLDCLRRYLQGLPVYERIDFEKT"
     gene            1951..2781
                     /locus_tag="VNU03_25875"
     CDS             1951..2781
                     /locus_tag="VNU03_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWP77977.1"
                     /translation="MKTPAEKAARFRALHERPGAFIIPNPWDAGTARLLASLGFEALA
                     TTSLGLANALGRVDGTNAVSRAEVIENCRVIAAATDLPVNADLENGYADEPRAAAEMI
                     RLAAEAGISGGSIEDATGDPARPIYDFDLAVERVHAAVEVARALPVPFLLTARAENLL
                     HGRDLDDAIRRLQAFERVGADVLYAPGVRDLTAIRAVVSAVGKPVNVVMSAADPDLTL
                     AQLAEAGVKRISVGGALSRLALAAFLRGAREMADRGGFTWIRDTVPTRDLKAVFREYP
                     "
     gene            2793..>3576
                     /locus_tag="VNU03_25880"
     CDS             2793..>3576
                     /locus_tag="VNU03_25880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HWP77978.1"
                     /translation="MRLRSHLVALVLASLVPVLAFAALVMRENAHLQLAATERGMRET
                     VAALAHTVDKELESTIAALEVLGQSEHLDVMNLPAFYALCDRVTRQQGLVNMVLFEAD
                     GRALFQTSVPLGTPRPATRRPDIFQQMRADRRPVISNLFDGALTRHIVSVYLPIAREG
                     AVRWVLAAGVRASDFGQLLRSQRFAPDTVAVLQDRDHVILARTQAEAETVGTHVQSPN
                     PGTDGWVRSRLREGVDVYVAFTTAPLSGWRVVLTQPVATVDAP"
BASE COUNT          514 a         1314 c         1252 g          496 t
ORIGIN      
        1 ctcgcccatc ccggggggaa cgtcacgggg ctgtcggatg cccacgccga cctcgtcccg
       61 aagcggctcg agctgctcaa gcaggtcgtc ccgtcggtca cccgggtcgg catcctgtgg
      121 agtcccgcca atccgggcac cgcgccacag gtgaggatcg ccgagggcgc cgctcccgcc
      181 ctgggcttga ccgttctccc ggtcgcggtc aagggccctc gacgtgacga cctggaccgg
      241 gcgttcgcca cgatcgagag agagcgtctc gggagtctct tcgtcatcgg ggactcgacc
      301 ctcggagttc aacgggatcg gctcgcggag ctctcgctga agcaccgcct cccgacgtcc
      361 gccagccata gccgatgggc ggagggtgga ctgctgatgt cgtacggcac cgacttcgtc
      421 gacctgttcc gccgcagcgc gagcctggtg gaccgtatcc tcaaaggcgc caagccggcc
      481 gatcttcccg tcgagcagcc gacgaagttc gagctcgtga tcaacatgcg caccgcgaag
      541 gcactcggcc tgacgatctc gccctcgttg ctgtcgcgcg cggatcgggt catcgagtaa
      601 acagaagtgg gagtaaacgg aggtgggagt acacggaggt gggcgtacac ggaggtggga
      661 gagtgatgcg catcgaggtg atctgcaccg gtgacgaggt gctgaccggc aagatcgtga
      721 acaccaactt cagctacatc acccagaagc tggaggacgt gggcctctcg gtcgactggg
      781 agaccaccgt cggcgacgac cgggagaacc tgctgcgcgc cttccacctg gccggcgagc
      841 gcgccgacgc ggtgatcgtg aacggtggtc tcggccccac cgtggacgat ctctcccagg
      901 agatcgcggc caaggcggcc ggggtggaac tggccctgaa cgaggactgg ctcacgcgca
      961 tggaggattt cttccagcgc cgcagccggg tgatgccgcc caacaaccgc aagcaggcca
     1021 tgctgcccac caccgccgag gtgatcgaca accccatcgg caccgcgtgc ggcttcgcgg
     1081 tggacatcgg ccgggcccgc ttctacttca cccccggcgt gccgcgcgag ctgcgccgga
     1141 tgctggagga gcagatcgtc ccgcgcctgc tcgccaaggc cgggctgccc ggcgcgatct
     1201 tcctcaagcg cttccactcc tacggcctgg gcgaatcgca cgtggactcg ctcctgaccg
     1261 gggtggaggc gctcgtgccg gacggcagcg tgaagctcgg cttccgcgcc cactacccgc
     1321 agctcgagac caagctgacc gcgcgcgggc gcgacatgga cgacatccgc cgcaagctcg
     1381 agccgatcga gcgcgaggtg cgcaagcgca tcggcaactt catcctggcc gaggacgacc
     1441 agaccctgga gagcgtgctg ctccgcgacc tgcgcgccca gggggcgacg ctctccctcg
     1501 tcgagacgtt cacgagcggg cagatggccg cgcgcctggc gcatctgccc ggggcggaga
     1561 cgatcttccg gcggggcacc atcgcgcggt ccctgcccga ggtgtgggcc gcggtcgggc
     1621 tcgcgggcgc gccgcccgaa ggcccgctca cgccgcaggt ggcggagacc gtcgcccgcg
     1681 ccgcgcgcgc gcagaccggg gccacccacg ccctcgcggt gctgatcgac ctcgacgacg
     1741 ggcccgaccg catcgagttc ggcgggacca tctgcctggc catcgccacc gcgtcggacg
     1801 ccgcctcgcg gaaggcgcgc atcctgggcg gccgggagtg ggtccgcctg ggcgcagtcg
     1861 agatgggcct cgactgtctc cgtcgctatc tccaggggct gccggtgtac gagcgcatcg
     1921 acttcgagaa gacgtgagga cgacccgcgc atgaagaccc ccgccgagaa ggccgcgcgc
     1981 ttccgggccc tgcacgagcg cccgggcgcc ttcatcatcc ccaacccgtg ggacgcgggc
     2041 accgcccgcc tgctcgcctc gctcggcttc gaggccctgg ccaccacgag cctcgggctc
     2101 gccaacgcgc tgggccgggt cgacggcacc aacgcggtga gccgggccga ggtcatcgag
     2161 aattgccggg tgatcgcggc cgcgaccgac ctgccggtga acgcggacct cgagaacggc
     2221 tacgcggacg agccgcgcgc ggcggcggag atgatccggc tcgcggcgga ggcgggcatc
     2281 tcggggggct cgatcgagga cgcgaccggc gacccggccc ggcccatcta cgacttcgac
     2341 ctcgcggtgg agcgggtgca cgccgcggtc gaggtcgccc gcgcgctccc ggtgcccttc
     2401 ctgctcaccg cgcgcgccga gaacctgctg cacgggcgcg acctcgacga cgcgatccgg
     2461 cggctgcagg ccttcgagcg agtgggcgcc gacgtcctct acgcgcccgg cgtccgcgac
     2521 ctgaccgcca tccgcgcggt ggtgtccgcg gtcggcaagc cggtcaacgt ggtgatgagc
     2581 gcggccgacc ccgacctcac cttggcccag ctcgcggagg cgggcgtcaa gcgcatcagc
     2641 gtggggggcg ccctctcgcg gctggccctc gccgccttcc tccggggagc gcgggagatg
     2701 gccgatcgcg gcggcttcac ctggattcgg gacaccgtgc ccacccgcga tctcaaggcg
     2761 gtattccggg agtacccctg atccggcgac ccatgcggct gcgcagccac ctcgtcgccc
     2821 tcgtcctcgc ctccctggtc ccggtgctgg ccttcgcggc gctcgtcatg cgcgagaacg
     2881 ctcacctgca gctcgcggcc accgagcgcg gcatgcgcga gacggtggcc gcgctcgccc
     2941 acacggtgga caaggagctc gagagcacca tcgcggcgct cgaggtgctg ggccagtccg
     3001 agcacctcga cgtgatgaac ctgcccgcgt tctacgcgct ctgcgaccgg gtgacgcgcc
     3061 agcagggcct ggtgaacatg gtgctcttcg aggcggacgg ccgcgccctc ttccagacct
     3121 cggtaccgct cggcaccccg cggccggcca cccggcgccc cgacatcttc cagcagatgc
     3181 gcgccgaccg gcgccccgtc atctcgaatc tcttcgacgg ggcgctcacc cgccacatcg
     3241 tgtcggtcta cctgccgatc gcgcgcgagg gggcggtgcg ctgggtgctc gcggccgggg
     3301 tccgggccag cgacttcggt cagctcctgc gctcccagcg gttcgccccg gacacggtgg
     3361 cggtgctcca ggatcgcgac cacgtgatcc tcgcccgcac ccaggcggag gccgagacgg
     3421 tcggcaccca cgtccagagc ccgaatccgg gaaccgacgg gtgggtgcga agccgcctgc
     3481 gcgagggcgt cgacgtctac gtcgcgttca ccaccgcccc gctctccggc tggcgcgtgg
     3541 tgctcaccca gccggtggcg acggtggacg cgccgc
//
LOCUS       DATMCV010001129         6565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_62656, whole genome shotgun sequence.
ACCESSION   DATMCV010001129 DATMCV010000000
VERSION     DATMCV010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62656"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VNU03_25885"
     CDS             complement(<1..356)
                     /locus_tag="VNU03_25885"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWP77979.1"
                     /translation="MQRQHERNLEILTALGEGTPVTQRALAARLGVALGLANLCLRRL
                     ASKGLIKVIEFPAKPSARKRLRYVLTPKGVAEKTRLSYEYMAYSLRLLRRTRGNLRET
                     MASLHAAGMKRFAIFRV"
     gene            401..1417
                     /gene="neuB"
                     /locus_tag="VNU03_25890"
     CDS             401..1417
                     /gene="neuB"
                     /locus_tag="VNU03_25890"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HWP77980.1"
                     /translation="MNSRTFVIAEAGVNHNGDPALARALVDAAVEARADAVKFQTFSV
                     DRLLTRDAAKAEYQQRATGREQTQYEMLARLQLSPADHEMLFAHCARVGIEFMSTPFD
                     PESARFLKRLGVRRLKISSGDVTNLPMLEVVGTLGLPVVLSTGMADMAEVEAAVATLR
                     AAGVTDLAVLQCVSDYPAAAALTNLKVMDSFARAFGTPVGLSDHSTGLTVAIAAVARG
                     AAYLEKHFTLDRSLPGPDHQASLLPAELRALVAAVREVESALGDGVKRPVPSELPVRD
                     VARKSLVAARDLAAGAVLGREDLDVLRPGTGLSPAALPAVLGRRTSRAIPHHTPITEE
                     MLSS"
     gene            1414..2406
                     /locus_tag="VNU03_25895"
     CDS             1414..2406
                     /locus_tag="VNU03_25895"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWP77981.1"
                     /translation="MITALVVGGGSIGKRHLRNLLATGRSAAVVETREDRRAEITAKH
                     PDVEVFTSLDQALAAGPFQAGFVCVPTAYHVPTALALARRGQHIFMEKPVSHSPDGVP
                     ELVDVLEQRGLVGMTAFCMRFFKPLQKAKELLDAGRIGRLVTARSFTGAYLPDWHPWE
                     DYRTFYMAKKDQGGGVLLDECHAFDWMQWLCGPITGVCAVAGTFSDLEVETDDVCEVV
                     ARFGDRALGSIHLDMVDRSIRTQVELIGTAGTVLVDLREHTVRAWDPETRKWETFTTE
                     RSYDQMYVEELAHFFDCIDRRAKPVVDLREGYQIQRVIDACARSSASGRWVTVP"
     gene            2467..3159
                     /locus_tag="VNU03_25900"
     CDS             2467..3159
                     /locus_tag="VNU03_25900"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HWP77982.1"
                     /translation="MVALGLVIARGGSKGLPRKNVLPLAGRPVLVHTIEAALASRALA
                     RAIISSEDAEILEMARRAGCPAPFVRPADLADDRSSTVAVALHALDWLEGHERFTPDV
                     VVLLPATTPLRAGEHIDGALETLHAHAEAEAVVAVTEPDYPPYWMLSLTDGRLSWLFP
                     EGGRVSRRQDLPQAYRPNGSIYAIRVPALRDQRTFYPRATRGYVMPREVSVNIDSEMD
                     FRLAELLLAKRS"
     gene            complement(3122..4396)
                     /locus_tag="VNU03_25905"
     CDS             complement(3122..4396)
                     /locus_tag="VNU03_25905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77983.1"
                     /translation="MKIAFLASRKGYLKVMGSLIQAALDHGHEALLLTDPGERKPGEA
                     TTAEDLAHWPRARVLPIQRGASLAPPLAREGAEALVGPSLHFVLSSIGSEEDIRAVRA
                     AGVRLYSVDYIVEALNSRPEAYRLLDRTFYTSEYERSWHWHGSPFKEDFAALGQSLRS
                     ARSAISGSTMLDQLGLVDRGAVRRHLGIGPDKPVVLFMSLKMAVPEPRRRFLWGDGSA
                     LTRTARAVVHGNAGLVPEIWKGNGYRALAHAVRRFCDRAGAALVVKSREKNRDPRFLR
                     NVADVFVERDDEVFPYTSIQLMGVADLCIHFQSGAVLEAAMAGVPSISIRVPLSDDQQ
                     KYPAHTFAFDVERGSLSNFPGVAWFTSPEGAVALLEGRTLADFRLDPAARRAYVERYL
                     GFDDLGSSRRVLQVIEDDARVSSASRGAAPPA"
     gene            complement(4393..5550)
                     /gene="neuC"
                     /locus_tag="VNU03_25910"
     CDS             complement(4393..5550)
                     /gene="neuC"
                     /locus_tag="VNU03_25910"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HWP77984.1"
                     /translation="MARTIGVVTVARSDYSHLVPLLEALRDAPDVVLQLYVAGAHLSP
                     RFGSTVKAIEADGWPITARVETTGASDAAVDVAAGTGAGVAGFARAFERGGPDLILLL
                     GDRLEMLAAALAALPLTIPVAHLHGGEVTEGAIDEQARHAITKLAHLHFPAAEPYARR
                     IRQMGEEPWRVHCVGAPGLDRFARGTALSREELARRLGLPLRRPTLLVTFHPVTLEPG
                     EAGRHSAELAAALEMVDGDVIVTYPGADAAHDAVIRPLESLAATRPGTRMVPALGEDG
                     YGALLREADVMVGNSSSGLIEAPSFGLPVVNVGIRQHGRLRAANVIDVGHGREEIVAG
                     IRRALAPEFRASLHGLVNPYGDGRASPRIARVLREVELGPRLIRKRFVDLA"
     gene            complement(5610..>6565)
                     /locus_tag="VNU03_25915"
     CDS             complement(5610..>6565)
                     /locus_tag="VNU03_25915"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HWP77985.1"
                     /translation="PAERNFVRFAGFVSFFPHLVAGPLTRHHQLIPQLARIARTAISP
                     RWREGVLLFSVGLCKKVLIADRLGNLIDPLLAPPLALDASRAWLGLLGYAFQIYFDFS
                     GYSDMAIGLGRLFGIELPQNFNSPYKATSPSDFWRRWHMTLSQWLRDYLYIPLGGNRC
                     SPARQRFNLMATMVLGGLWHGANWTFAAWGTWHGLLLVGHHVCPAWDRRLSPRWQRNA
                     TFLLVTLGWVFFRSETFDHAARWFGSLAGAHGLAPPWTSETLSLAALVALCLGLVRLC
                     PNSLELPLDRLGRLPQIGLATATAVAVLLMNYGSKFLYFQF"
BASE COUNT          984 a         2315 c         2360 g          906 t
ORIGIN      
        1 acgcggaaga tcgcgaagcg cttcatgccg gccgcgtgca ggctcgccat ggtctcccgg
       61 agattgccac gggtgcgccg caggagccgc aatgaatacg ccatgtattc gtaggagagc
      121 cgggtcttct cggccactcc cttcggcgtc agcacgtagc gcagtcgctt gcgcgccgag
      181 ggcttggccg ggaactcgat caccttgatg agacccttgc tcgccagccg ccgcaggcag
      241 agattcgcga ggcccaaggc cacgccgagc cgggccgcga gggctcgctg ggtcaccggg
      301 gttccctcgc cgagggcggt caggatctcg agattgcgct cgtgctggcg ctgcatggag
      361 aatgttcaca agttgaacac cgtggtgtaa gatgcgtcaa gtgaattccc gcacgttcgt
      421 catcgccgag gccggcgtca atcacaacgg cgatcccgcg ctcgcccgcg ccctggtcga
      481 cgcggcggtg gaggcgcggg ccgacgcggt caagttccag acgttcagcg tggatcgcct
      541 gctcacccgg gacgcggcca aggccgagta ccagcagcgg gccaccggcc gcgagcagac
      601 ccagtacgag atgctggcgc gcctccagct ctcccccgcc gaccacgaga tgctgttcgc
      661 ccactgcgcc cgggtcggca tcgagttcat gtccaccccg ttcgaccccg agagcgcgcg
      721 attcctgaag cgcctcgggg tccggcgcct caagatctcg tccggcgacg tgaccaacct
      781 gccgatgctg gaggtggtgg gcacgctcgg gctgccggta gtgctctcca ccggcatggc
      841 cgacatggcc gaggtggagg ccgcggtggc gaccctccgc gccgccgggg tcaccgacct
      901 ggcggtgctg caatgcgtga gcgactaccc cgccgcggcg gctctcacga atttgaaggt
      961 gatggacagc ttcgcgcggg ccttcgggac cccggtgggc ctgtccgatc attcgacagg
     1021 gctgaccgtg gcgatcgcgg ccgtcgcacg cggcgccgcg tacctcgaga agcacttcac
     1081 cctggaccgg agcctgcccg gccccgacca ccaggcctcc ctcctcccgg ccgagctgcg
     1141 ggcgctggtg gccgccgtcc gcgaggtcga atccgctctc ggcgacgggg tcaagcggcc
     1201 ggtcccgagc gagctgccgg tgcgcgacgt ggcccgcaag agcctggtgg cggcgcgcga
     1261 cctggccgcc ggcgcggtgc tcggccgcga ggacctggac gtgctccgtc cggggaccgg
     1321 gctgtcgccc gcggcgctgc ccgcggtgct cggccgccgg acctcgcggg ccatccccca
     1381 tcacactccg atcaccgagg agatgctctc gtcatgatca ccgctctcgt cgtcggcggc
     1441 ggctccatcg gcaagcgcca tcttcgcaac ctgctggcga ccggccggtc ggccgcggtg
     1501 gtcgagacgc gcgaggaccg ccgggccgag atcaccgcca agcacccgga cgtcgaggtg
     1561 ttcacgagcc tggaccaggc gctcgcggcc gggccgttcc aggccggctt cgtctgcgtg
     1621 cccaccgcgt accacgtgcc gaccgcgctg gccctggcgc gccggggcca gcacatcttc
     1681 atggagaagc cggtctccca ctcccccgac ggggtgcccg agctcgtgga cgtgctcgag
     1741 cagcgcgggc tcgtcggcat gaccgccttc tgcatgcgct tcttcaagcc gctccagaag
     1801 gcgaaggagc tgctcgacgc gggccgcatc ggccgcctcg tgaccgcgcg gagcttcacc
     1861 ggggcgtatc tcccggactg gcatccgtgg gaggactacc gcaccttcta catggccaag
     1921 aaggaccagg gcggcggagt gctcctggac gagtgtcacg ccttcgactg gatgcagtgg
     1981 ctctgcgggc cgatcaccgg ggtatgcgcg gtggccggga ccttcagtga tctcgaggtg
     2041 gagaccgacg acgtctgcga ggtggtcgcc cgcttcgggg atcgggcgct cgggtcgatc
     2101 cacctcgaca tggtggaccg ctcgatccgg acccaggtcg agctcatcgg caccgcgggg
     2161 acggtcctcg tcgatctgcg cgagcacacc gtgcgcgcct gggaccccga gacccgcaag
     2221 tgggagacgt tcacgaccga gcggagctac gaccagatgt acgtggagga gctcgcgcac
     2281 ttcttcgact gcatcgaccg gcgcgcgaag ccggtggtgg acctgcggga gggataccag
     2341 atccagcgcg tcatcgacgc ctgcgcccgc tccagcgcct cgggccgctg ggtgaccgtc
     2401 ccctgacatg acggtggccg cggccggcgt gcgggccgaa tgcacccgcc gccccggagc
     2461 gcgcgcgtgg tcgccctagg cctcgtcatc gcgcggggcg gctcgaaggg gctgccgcgg
     2521 aagaacgtcc tccccctggc cggccgaccg gtgctcgtcc acaccatcga ggccgcgctc
     2581 gcctctcgcg cactcgcccg cgccatcatc tcctcggagg acgcggagat cctcgagatg
     2641 gcccgccgcg cgggatgccc ggcgccgttc gtgaggcccg ccgacctggc ggacgatcgc
     2701 agctccacgg tggcggtcgc gctccacgcg ctcgattggc tcgagggcca cgagcgcttc
     2761 acccccgacg tcgtggtgct cctgcccgcg accaccccgc tgcgggccgg cgagcacatc
     2821 gacggggccc tggagacgct acatgcgcac gcggaagccg aggcggtggt ggcggtcacc
     2881 gagcccgact atccgcccta ctggatgctc tcgctgaccg acggacggct ctcctggctc
     2941 ttcccggagg gcggtcgcgt gtcccggcgg caggatctgc cccaggccta tcggccgaac
     3001 gggtcgatct acgcgatccg ggtgcccgcc ctccgtgatc agcgcacctt ctacccgcgg
     3061 gccacgcggg gctacgtgat gccccgggag gtctcggtca acatcgactc ggagatggac
     3121 ttcaggctgg cggagctgct cctcgcgaag cggagctgac ccgcgcgtcg tcctcgatca
     3181 cctgcagcac gcgccggctg ctgcccaggt cgtcgaatcc cagatagcgc tcgacgtagg
     3241 cgcgacgcgc ggcgggatcc aggcggaagt cggcgagcgt ccgcccctcg agcagcgcca
     3301 ccgctccctc gggagaggtg aaccacgcga cgcccggaaa gttcgagagg ctgccccgtt
     3361 cgacatcgaa ggcaaacgtg tgggcggggt acttctgttg atcgtccgac agtggcaccc
     3421 tgatactgat ggagggcact ccggccatgg cggcctcgag gacggcgcca ctctggaaat
     3481 ggatgcaaag gtcggctacc cccatgagct ggatggaagt atagggaaac acctcgtcgt
     3541 cgcgctcgac gaagacgtcg gcgacgttcc ggagaaatcg gggatcccgg ttcttctccc
     3601 tggatttcac gaccagggcg gcacccgccc ggtcacagaa gcggcggaca gcgtgggcca
     3661 gcgctcggta tccgttcccc ttccagatct cggggacgag accggcgttg ccgtggacga
     3721 cagccctggc cgtccgggtg agcgccgacc cgtcgcccca gaggaatcgc cgacggggct
     3781 cggggacggc catcttgagc gacatgaaca gcaccactgg tttgtccggc ccgattccca
     3841 ggtgacgcct gaccgccccc cgatcgacca ggccgagctg gtccagcatg gtggagccgc
     3901 ttatcgcgct gcgagcagac cgcaggctct ggcccagcgc agcgaagtct tccttgaacg
     3961 ggctcccgtg ccagtgccag ctccgctcgt actcgctcgt atagaacgta cggtccagca
     4021 ggcggtaggc ctccgggcgg ctgttcagcg cctccacgat gtagtcgacg gagtacagcc
     4081 ggacccccgc ggcccgcacg gcccggatgt cctcctccga tccaatgctg gagagcacga
     4141 agtgcagcga cggccccacc agggcctcgg caccctcgcg cgcgagcggc ggcgccagtg
     4201 aggcgccccg ctggatgggc aggacgcgcg cgcggggcca gtgcgccagg tcctccgcgg
     4261 tcgtggcctc gcccggcttc cgctcgcccg gatcggtcag cagcagggcc tcgtgcccgt
     4321 ggtcgagcgc ggcctggatg agcgagccca tgaccttgag atagcccttg cgggaggcga
     4381 ggaaggcgat cttcacgcga ggtccacgaa gcgcttgcgg atcagccgcg ggccgagctc
     4441 gacctcgcgg agcacccgcg cgatgcgcgg cgaggcgcgc ccgtcgccgt agggattcac
     4501 gagaccgtgc aggctcgcgc ggaactcggg cgcgagggcc cgccggatcc cggccacgat
     4561 ctcctcgcgc ccgtgaccca cgtcgatcac gttggccgcg cgcagtcggc cgtgctggcg
     4621 gatgcccacg ttcaccacgg gcaggccgaa ggacggcgcc tcgatgaggc cgctcgacga
     4681 gttccccacc atcacgtccg cctcccggag gagcgcgccg tacccgtcct cgccgagggc
     4741 cgggaccatg cgcgtgccgg ggcgggtggc cgcgagcgat tccagggggc ggatcaccgc
     4801 atcgtgcgcg gcgtcggcgc cggggtaggt gacgatcacg tcgccgtcca ccatctcgag
     4861 ggcggcggcc agctccgcgc tgtgccggcc cgcctcgccg ggctcgaggg tcaccgggtg
     4921 gaaggtcacc agcagggtcg gccggcgcag cggaagcccg agccgccggg ccagctcctc
     4981 gcgcgacagc gcggtacccc gcgcgaaccg atcgagcccg ggagccccca cgcagtgcac
     5041 ccgccacggc tcctccccca tctgccggat gcgccgcgcg tagggctccg cggcgggaaa
     5101 gtgcagatgg gcgagcttcg tgatcgcgtg ccgcgcctgc tcgtcgatgg cgccctcggt
     5161 gacctcgccg ccgtggagat gggcgaccgg gatcgtgagg ggcagcgcgg ccagcgccgc
     5221 ggccagcatc tcgaggcgat cgccgaggag caggatcagg tcgggcccgc cccgctcgaa
     5281 cgcgcgggca aatccggcga cgcccgcgcc ggtccccgcc gcgacgtcga ccgccgcgtc
     5341 ggaggcgccg gtcgtctcga cgcgggcggt gatcggccac ccgtcggcct cgatggcctt
     5401 cacggtgctc ccgaagcgcg gcgacagatg cgcgcccgcc acgtacagct gcaggacgac
     5461 gtccggcgca tcgcggagcg cctcgaggag gggaaccagg tgggagtagt cggagcgagc
     5521 cacggtgacg actccgatag tcctcgccat gctcccggat tctagcacgc gctcgcgggc
     5581 gggccgttcg tcgccggcgc ggaactggct cagaactgga agtagagaaa cttggagccg
     5641 tagttcatca gcagcaccgc gaccgcggtc gcggtggcga ggccgatctg cggcaggcgc
     5701 cccaggcggt cgagcgggag ctcgaggctg ttcgggcaga ggcggacgag gccgagacag
     5761 agggccacca gcgcggcgag cgagagcgtc tcgctcgtcc acggcggcgc gagcccatgc
     5821 gcgccggcga ggctcccgaa ccatcgggcc gcgtggtcga aggtctcgga gcggaagaag
     5881 acccagccga gggtcacgag caagaacgtg gcgttgcgct gccagcgggg cgagagccgc
     5941 cggtcccagg ccggacagac gtggtgcccg accagcagca ggccgtgcca ggtgccccat
     6001 gccgcgaacg tccagttggc cccgtgccag agcccgccca gcaccatggt ggccatcagg
     6061 ttgaaccgct ggcgggccgg cgagcagcgg ttccccccca gggggatgta gaggtagtcg
     6121 cgcagccact gggagagcgt catgtgccat cgccgccaga agtcgctggg cgacgtggcc
     6181 ttgtaggggc tgttgaagtt ctggggcagc tcgatcccga agagccggcc gagcccgatc
     6241 gccatgtcgg agtagccgct gaagtcgaag tagatctgga aggcgtaccc gaggaggccg
     6301 agccaggcgc gggaggcgtc cagcgccagc ggcggggcga gcagcggatc gatgaggttc
     6361 ccgaggcgat ccgcgatcag gaccttcttg cagagcccga cgctgaagag caggacgccc
     6421 tcgcgccagc gcgggctgat agcggtgcgc gcgatgcgcg ccagctgggg gatgagctgg
     6481 tgatggcggg tgagcgggcc cgccaccagg tggggaaaga agctcacgaa gcccgcgaag
     6541 cggacgaagt tgcgctcggc gggcg
//
LOCUS       DATMCV010001130         2907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_332209, whole genome shotgun sequence.
ACCESSION   DATMCV010001130 DATMCV010000000
VERSION     DATMCV010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332209"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VNU03_25920"
     CDS             complement(<1..291)
                     /locus_tag="VNU03_25920"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWP77986.1"
                     /translation="MPESADVLSPFPRDRTWLLPALHALQHRDRWLSPEALAAAADHL
                     RVPRSEVYGVATHYPEFRQSRPGRRVVRVCVGLSCRARGALDVLAACERRLGI"
     gene            complement(305..997)
                     /gene="queC"
                     /locus_tag="VNU03_25925"
     CDS             complement(305..997)
                     /gene="queC"
                     /locus_tag="VNU03_25925"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWP77987.1"
                     /translation="MTGRRAVVLLSGGLDSATALAIAAAEGFDAYALSFDYGQRHDRE
                     LDSARRVATALGAKEHLVLRLDLRAIGGSALTADIPVPKGRSAEAMGAGIPVTYVPAR
                     NTIFLSHALAWAEVLESEDIFIGVNALDYSGYPDCRPEYIEAFERMANLATRAGVEGS
                     SRLQIHTPLIRLTKAEIVARGAGLGVDFSLTWSCYEPRPDGLACGLCDSCLLRAKGFR
                     EAGLRDPAPAAP"
     gene            complement(997..1686)
                     /locus_tag="VNU03_25930"
     CDS             complement(997..1686)
                     /locus_tag="VNU03_25930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HWP77988.1"
                     /translation="MLAFPTVPAISGRVAETFYSIQGEGATAGQPAIFVRLQGCSVGC
                     GWCDTKYSWDPAGGREVALPAVLDEVAAFPCRRVVVTGGEPLESALFAPLIGALAGRG
                     YTVEVETSGTLPPPTDAPPSIQWNVSVKLAGSGVAEERRINAGAIRGFLARDAWWKFV
                     VSEPSEIGEVLQLTERFALPRERVLLQPEGLRPEELAARSPWLVEACKAHGFRFSPRL
                     HVLIWGARRGV"
     gene            1773..2048
                     /locus_tag="VNU03_25935"
     CDS             1773..2048
                     /locus_tag="VNU03_25935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77989.1"
                     /translation="MAYSLTDSLKFNRFQVVSVDTAASTLRVRGESEACTDFMCGEAL
                     VVTDEGTTRDLERLHPGDIVTLDHKDGRAQQIRVVRRVWDEYSSPEW"
     gene            complement(2053..2769)
                     /locus_tag="VNU03_25940"
     CDS             complement(2053..2769)
                     /locus_tag="VNU03_25940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HWP77990.1"
                     /translation="MSDVLDGVDKARFVSAMFSRIARRYDLMNSLMTFGMHHAWRALA
                     ARQTIASPDGPALDLATGTGDLALELAELHPHRAVIGADFSLGMLAVARDKMRGLEEA
                     RRVRLMAADALALPFEARTFACVTSAFLLRNLADLEQGLREMRRVSRPGGRVVALEIT
                     QMTAPGFSTLFRLYFHHVVPRVGQLVGGDREAYTYLPQSVDRFVTPGELSALMEKVGL
                     RGVGYRRLGLGTVTVHTGIA"
BASE COUNT          450 a         1032 c         1077 g          348 t
ORIGIN      
        1 gatgccgagg cgccgctcgc aggccgccag cacgtcgagg gcgccgcgcg ctcgacagga
       61 gagccccacg cagacgcgca ccacgcgacg ccccgggcgg ctctgccgga actcgggata
      121 gtgggtggcc acgccgtaga cctcgctacg cgggacccgc aagtgatcgg cggcggccgc
      181 gagcgcctcg ggcgagagcc accggtcgcg gtgctgcagc gcgtgcagcg ccggcagcag
      241 ccacgtgcgg tcgcgcggga acgggctcag gacgtcggcg gactcgggca cgggccgggc
      301 gggctcaggg cgcggcaggg gcgggatcgc gcaggccggc ctcgcgaaag cccttggcgc
      361 ggagcaggca ggaatcgcag agcccgcacg ccaggccgtc gggccgcggc tcgtagcagg
      421 accaggtgag cgagaagtcg acgccgagcc cggcgcctcg cgcgacgatc tcggccttgg
      481 tcaggcggat cagcggcgtg tggatctgca gccggctgga gccctcgacc ccggcgcgcg
      541 tggcgaggtt cgccatgcgc tcgaaggcct cgatgtactc cggccggcag tccggatagc
      601 cactgtagtc gagcgcattt accccgatga agatgtcctc cgactcgagg acttccgccc
      661 aggccagcgc gtggctcagg aagatggtat tccgggccgg cacgtaggtg accgggatgc
      721 ccgcgcccat ggcctcggcg ctgcggccct tgggcaccgg gatgtcggcg gtgagtgccg
      781 agccgccgat cgcgcgcagg tcgagccgca gcacgaggtg ctccttggcg ccgagcgcgg
      841 tggccacccg gcgcgccgag tcgagctcgc ggtcgtgccg ctggccgtag tcgaacgaga
      901 gcgcgtaggc gtcgaagccc tcggctgcgg cgatcgcgag ggcggtggcc gagtcgaggc
      961 cgcccgagag caggaccacc gcgcggcgac cggtcatcag accccccgcc gggcgcccca
     1021 gatcagcacg tgcagccggg gcgagaagcg gaacccgtgg gccttgcagg cctcgacgag
     1081 ccagggggag cgcgcggcca gctcctccgg gcgcaggcct tccggctgga gcagcacccg
     1141 ctcacgcggc agcgcgaagc gctcggtcag ctgcagcacc tcgccgatct cgctgggctc
     1201 gctcaccacg aatttccacc aggcgtcacg ggcgaggaag ccgcggatgg cgcctgcgtt
     1261 gatccgtcgc tcctccgcca ccccggagcc ggcgagcttc accgagacgt tccactggat
     1321 ggatgggggc gcgtcggtcg gcggcggcag ggtgccggag gtctcgacct cgacggtgta
     1381 gccgcggccg gccagcgcgc cgatcagcgg agcgaagagc gcggactcga gcggctcgcc
     1441 accggtgacg accacccggc ggcacgggaa ggcggcgacc tcgtcgagca ccgcgggcag
     1501 cgctacctcg cggccgccgg cgggatccca ggagtacttc gtgtcgcacc acccgcagcc
     1561 gaccgagcag ccctgcaggc gcacgaagat ggcgggctga ccggcggtgg caccttcacc
     1621 ctggatcgag tagaacgtct cggcgacgcg cccgctgatg gccggcacgg tcggaaatgc
     1681 tagcacggct agcggctcgg ggcgcagatg gcccttgccg ggccccggag ccctttgata
     1741 taacaggcgg gatcggaaag gaggaccctc ccatggccta cagcctgacc gattccctca
     1801 agttcaaccg gttccaggtg gtatccgtcg acacggcggc gagcacgctc cgcgtgcggg
     1861 gcgagtccga ggcctgtacc gacttcatgt gcggcgaggc cctcgtggtc accgacgagg
     1921 gcaccacccg cgacctcgag cggctgcacc ccggtgacat cgtcacgctc gaccacaagg
     1981 acggtcgcgc gcagcagatc cgggtggtgc ggcgcgtctg ggacgagtac tccagccccg
     2041 agtggtgagc gctcaggcga tgccggtgtg cacggtgacg gtgccgagcc cgagccgccg
     2101 gtagcccacg ccccgtagcc cgaccttctc catcagggcg gatagctcgc ccggcgtcac
     2161 gaagcgatcc accgactgcg gcaggtacgt gtaggcctcc cggtcgcccc ccaccagctg
     2221 gcccacgcgc ggcaccacgt ggtggaagta gagacgaaag agggtcgaga acccgggcgc
     2281 ggtcatctgg gtgatctcga gcgccaccac ccggccgccc ggccggctca cccgccgcat
     2341 ctcccgcagg ccctgctcga ggtcggccag gttccgcagc aggaacgcgc tcgtgacgca
     2401 cgcgaacgtc cgcgcctcga agggcagggc cagcgcgtcc gcggccatca ggcggacccg
     2461 gcgcgcttcc tcgagcccgc gcatcttgtc gcgcgcgacc gcgagcatgc cgagcgagaa
     2521 gtcggcgccg atcaccgcgc ggtgcgggtg cagctcggcc agctcgagcg cgaggtcgcc
     2581 ggtgccggtg gccaggtcca gcgccgggcc gtccggggag gcgatggtct gccgggcggc
     2641 cagggcgcgc cacgcgtggt gcatgccgaa ggtcatgagg ctgttcatca ggtcgtagcg
     2701 gcgcgcgatg cgcgagaaca tcgcgctgac gaaccgcgcc ttgtccacgc cgtccaggac
     2761 gtcgctcacg ccaggcggcc cgcgatcacc gagaggagca ggatcaccgc gatgtagccg
     2821 ttgacgttga agaacgccac gtcgagccgc gagagatcgc ccggcgagac gagcgagtgc
     2881 tcgtagacca gcagccccgc gaccacc
//
LOCUS       DATMCV010001131         2872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_265177, whole genome shotgun sequence.
ACCESSION   DATMCV010001131 DATMCV010000000
VERSION     DATMCV010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265177"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VNU03_25945"
     CDS             complement(<1..260)
                     /locus_tag="VNU03_25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWP77991.1"
                     /translation="MGEFLLVTIQVEMHDRLVMVLQDELTQQIVQHRARGVLIDISAL
                     EIVDSFIGRALGNIAKMARVLDADTVVGGMRPAVAITLVELGL"
     gene            complement(298..1155)
                     /locus_tag="VNU03_25950"
     CDS             complement(298..1155)
                     /locus_tag="VNU03_25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWP77992.1"
                     /translation="MVDHRSRLASILREHEAHILADWITSLAKATRGGLDRAEVEEQA
                     RAFVGLLADAMSRDGGGEIESPAWAKVREMLADLSSARARQGRPPSETATFIFSLKEP
                     LFARLREEYRGDAEALARELWTATLLLDKLGLLTTEAYQKTREDLIARQQREMLELST
                     PVVALWENILAVPLIGTLDSERTQVVMENLLQRIVDTGASIAIIDITGVPTVDTLVAQ
                     HLLKTVGAARLMGAECIISGIRPQIAQTIVHLGVDLGNVATKATLADALVLALARVGA
                     AVTRTAPRA"
     gene            1613..>2872
                     /locus_tag="VNU03_25955"
     CDS             1613..>2872
                     /locus_tag="VNU03_25955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWP77993.1"
                     /translation="MLSVVLALGLAGALLPLPVRPAVVEAAEGQATWAVHITLAPTWF
                     DPAETSGIITPFMVLYALHDALVKPMPGNPMAPSLAESWTASKDGLVYEFVLRKNVRF
                     HNGEVMTAEDVKFSFERYRGASASLLKSKVSKVEVVDPLRVRFTLKQAWPDFLPFYAT
                     PATGAAWIVPKKYVEQVGEEGFKKAPVGAGPYKFVAFKPGVELTLEAHEAYWRKPPAV
                     KTLVFRTIPDESTRLAALTRGEVDVAYSITGPLAEEVKRTPALRLVPTYFLFTTWLVF
                     PDQWDPKSPWHDRRVRLAANLAIDRAGINQAVYLGLSKLSYSFIPQGMEYFWSPPPYP
                     YDPKRARALLAEAGYPNGFETGDLSGDTIYGVAIGEPVANYLQQVGIRLRLRPMERAA
                     FFKEYGEKKLRGVIFSGSGAPGNAPTRL"
BASE COUNT          425 a         1016 c          991 g          440 t
ORIGIN      
        1 agaccgagct cgacgagggt gatggccacc gcgggccgca tgccccccac cacggtgtcc
       61 gcgtcgagga cgcgggccat cttggcgatg ttgccgaggg cgcggccgat gaacgagtcg
      121 acgatctcga gcgcggagat gtcgatcagc actccgcggg cccggtgctg gacgatctgc
      181 tgggtcagct cgtcctggag caccatcacg agccggtcgt gcatctcgac ctggatggtg
      241 acgagcagga actcgcccat cttgaggatg gggatccgct cggcgggcgg cttgccgtca
      301 agcacggggg gcggtgcggg tcaccgcggc gccgacgcgc gcgagggcca gcaccagcgc
      361 gtcggcgagg gtggccttgg tggcgacgtt accgaggtcg acgcccaggt ggacgatggt
      421 ctgggcgatc tggggccgga tgccgctgat gatgcactcg gcgcccatca gccgggccgc
      481 ccccaccgtc ttcagcaggt gctgggcgac cagcgtgtcc accgtcggca cgccggtgat
      541 gtcgatgatc gcgatggagg cgccggtgtc cacgatgcgc tggagcaggt tctccatcac
      601 cacctgggtg cgctcgctgt cgagcgttcc gatgagcggc accgcgagga tgttctccca
      661 gagcgcgacc accggggtgg acagctcgag catctcgcgc tgctgacgcg cgatgaggtc
      721 ctcgcgcgtc ttctggtacg cctcggtggt cagcaggccg agcttgtcga gcagcagggt
      781 ggcggtccac agctcgcgcg cgagcgcctc cgcgtcgccg cggtactcct cgcgcagccg
      841 cgcgaagagg ggctccttga gcgagaagat gaaggtcgcg gtctccgacg gggggcggcc
      901 ctggcgcgcc cgggcactgg agaggtccgc gagcatctcg cggaccttgg cccaggcggg
      961 gctctcgatc tcgcccccgc cgtcgcgcga catggcgtcg gcgagcagcc cgacgaacgc
     1021 gcgggcctgc tcctccactt ccgctcggtc gagtccgcca cgggtcgcct tcgcgagcga
     1081 cgtgatccaa tcagcgagga tgtgtgcctc gtgctcgcga aggatcgacg cgagccggct
     1141 ccggtggtcc accatggtcc gctcctgttc gaggaaggat tgagctcggg tcgcgaggga
     1201 aaccatttaa cgcgtacgcc gcgtgctttt caacaattgt tagccgtcga gagtgggtgt
     1261 cggcgcgtcg gtcgcgcata cggtaaatcg ttccagccct cggctccggt cgccgccacc
     1321 ggctcgctcc accggccggt aagaactacc gccgggtgcc ggcccgggcg cgaggccggc
     1381 cattgacaag acgggcgttt cgtcgataat cgcggcaccc gcagaccaga atcaggcccg
     1441 atcaatttct ctacccgctc ccgcccttcg tctcatggag gaggcgatgg tgatgaccgg
     1501 acaacggcgg ataacgcccg acggccctcg cggcggcccg cacggccgtc ccggccacgg
     1561 gtctgccgcc cgccgcccgc gtgaggcgac ccggaccgtg agacccggtc gcgtgctctc
     1621 cgtcgtcctg gccctggggc tcgccggggc gctcctcccc ctcccggtcc ggcccgccgt
     1681 ggtcgaggcc gccgagggac aggccacgtg ggcggtgcac atcacgctgg ccccgacgtg
     1741 gttcgacccc gcggagacct ccggcatcat cacgcccttc atggtcctct acgcgctgca
     1801 cgacgccctg gtgaagccga tgccgggcaa tccgatggcc ccgagcctgg ccgagtcgtg
     1861 gaccgcctcg aaggacggcc tggtgtacga gttcgtgctc cgcaagaacg tccgcttcca
     1921 caacggcgag gtcatgaccg ccgaggacgt gaagttctcc ttcgagcgct accggggcgc
     1981 gagcgcgagc ctactcaaga gcaaggtctc caaggtggag gtggtggacc ccctgcgggt
     2041 ccgcttcacc ctcaagcagg cctggcccga cttcctgccc ttctacgcca cgccggccac
     2101 cggcgcggcc tggatcgtgc cgaagaagta cgtggagcag gtgggcgagg agggcttcaa
     2161 gaaggcgccg gtgggcgcgg gaccgtacaa gttcgtcgcg ttcaagcccg gcgtggagct
     2221 gaccctcgag gcccacgagg cgtactggcg gaagcccccc gcggtgaaga ccctcgtgtt
     2281 ccgcacgatc cccgacgagt cgacgcgcct ggccgcgctc acgcgcggcg aggtggacgt
     2341 ggcctactcg atcaccggcc cgctggccga ggaggtcaag cgcacccccg ccctccgcct
     2401 ggtgccgacc tacttcctgt tcaccacctg gctcgtcttc cccgaccagt gggacccgaa
     2461 gtcgccgtgg cacgaccggc gggtgcgcct ggccgccaac ctggcgatcg atcgcgccgg
     2521 catcaaccag gccgtctacc ttggcctctc caagctctcc tactcgttca tcccgcaggg
     2581 catggagtac ttctggagcc cgccgcccta tccatacgac cccaagcggg cccgagcgct
     2641 gctcgcggag gcgggctatc cgaacggctt cgagaccggc gacctctcgg gcgacacgat
     2701 ctacggggtg gccatcggcg agccggtggc caactacctg cagcaggtcg gcatccggct
     2761 ccggctgcgg cccatggagc gggccgcctt cttcaaggag tacggcgaga agaaactccg
     2821 cggcgtcatc ttcagcggca gcggcgcgcc cggcaacgcc cccacccgcc tc
//
LOCUS       DATMCV010001132         2092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_265212, whole genome shotgun sequence.
ACCESSION   DATMCV010001132 DATMCV010000000
VERSION     DATMCV010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265212"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VNU03_25960"
     CDS             complement(<1..1225)
                     /locus_tag="VNU03_25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWP77994.1"
                     /translation="MRRTGTVAITLLLCAIALVSVPAAFAQTTPAAPAPAAAATPAPA
                     PAKIDSGDTAWVLMSTALVLLMTAPGLALFYGGMVRQKNALGTLMQSFIILALISVQW
                     VLWGYSLAFGPDKGGIIGGLEWVGLSGVGATPNADYAATIPHTAFMLFQMMFAVITPA
                     LITGAFAERKKFSTFLVFILVWATVVYDPLAHWVWGVGGWLRNLGALDFAGGTVVHIS
                     SGISALAAALVIGKRKGYGHQPMPPHNLPFTVMGASLLWFGWFGFNAGSALAANGLAA
                     HAFMTTNTATAAATLGWMFTEWSSRGKPTVLGAASGAVAGLVAITPAAGYVTPMAAIV
                     IGAVAGIICYSACNLKSKLGYDDSLDVVGVHGVGGTWGALATGIFATKLVNDAGGDGL
                     LYGNPKQLGVQLLAVL"
     gene            complement(1252..1590)
                     /locus_tag="VNU03_25965"
     CDS             complement(1252..1590)
                     /locus_tag="VNU03_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWP77995.1"
                     /translation="MKKIEAIIKPFKLDDVKEALTGIGVIGMTVSEVRGFGRQKGHTE
                     LYRGGEYTVDFLPKIKVEVVVPDNLADKVAGVVAGAAKTGNIGDGKIFIQPVDTAVRI
                     RTGERDESAL"
     gene            complement(1769..>2092)
                     /locus_tag="VNU03_25970"
     CDS             complement(1769..>2092)
                     /locus_tag="VNU03_25970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWP77996.1"
                     /translation="GYQALSVLGAVRPDVIVLDLRMPRMDGMDFLQRLSRRSPRPKVI
                     VCSGSFTEFERPHIQGVQLAAKTADLKELWAALRSVVPASAVEAMAPAKHAVAVEDAN
                     AVWRD"
BASE COUNT          338 a          721 c          704 g          329 t
ORIGIN      
        1 ccagcaccgc gagaagctgg accccgagct gcttcgggtt gccgtagagg agcccgtcgc
       61 cgcccgcgtc gttcacgagc ttggtggcga agatcccggt ggcgagggcg ccccaggtgc
      121 cgcccacccc gtgcacgccc accacgtcga gggagtcgtc gtagccgagc ttcgacttga
      181 gattgcacgc cgagtagcag atgatcccgg ccaccgcgcc gatcacgatc gcggccatcg
      241 gggtcacgta gcccgccgcg ggagtgatgg cgaccagccc ggccaccgcg ccggaggccg
      301 cccccaggac cgtcggcttg ccgcgcgacg accactcggt gaacatccag cccagcgtgg
      361 ccgcggcggt cgcggtgttg gtggtcatga acgcgtgcgc cgcgagtccg ttggcggcga
      421 gcgcgctccc cgcgttgaag ccgaaccacc cgaaccacag gaggctcgct cccatcaccg
      481 tgaacggcag attgtggggc ggcatcggct ggtggccgta gcccttgcgc ttgccgatga
      541 ccagggcggc ggcgagcgcc gagatgccgg aggagatgtg caccacggtg ccgcccgcga
      601 agtcgagcgc ccccaggttg cggagccacc cgccgacccc ccacacccag tgggccagcg
      661 ggtcgtagac caccgtcgcc cagaccagga tgaagaccag gaaggtggag aacttcttcc
      721 gctccgcgaa ggccccggtg atgagcgcgg gcgtgatcac cgcgaacatc atctggaaca
      781 gcatgaaggc ggtgtgcggg atggtcgccg cgtagtccgc gttcggcgtc gctccgaccc
      841 cgctgagccc gacccactcg agaccgccga tgatcccgcc cttgtcgggt ccgaaggcca
      901 ggctgtagcc ccagagcacc cactgcaccg agatcagggc caggatgatg aagctctgca
      961 tcagggttcc cagcgcgttc ttctgccgga ccatcccgcc gtagaacagg gcgaggccgg
     1021 gggcggtcat gagcagcacg agcgcggtag acatcaggac ccaggcggtg tcgccgctgt
     1081 cgatcttggc cggcgcgggt gccggggtcg cggcggcggc cggagcggga gccgccgggg
     1141 tggtctgggc gaaggcggcc ggcacgctca cgagcgcgat ggcgcacagc aagagcgtga
     1201 tcgcgacggt tcctgtcctt ctcatcccgg tgatgacctc cctattgcgg ctcagagcgc
     1261 gctctcgtcc cgctcgccgg tccggatccg gaccgccgtg tccaccggct gaatgaagat
     1321 cttcccgtcg ccgatgttgc ccgtcttggc cgctcccgcg acgaccccgg ccaccttgtc
     1381 cgcgaggttg tcgggaacca cgacctccac cttgatcttc gggaggaagt cgaccgtgta
     1441 ctctccaccg cgatagagct cggtgtgacc cttctgccga ccgaacccac ggacctcaga
     1501 caccgtcatg ccgatgaccc cgattccggt cagggcctcc ttcacgtcat cgagcttgaa
     1561 gggcttgatg atggcttcga tctttttcat gtgagcctcc cgggcggcct gtctgcactc
     1621 gtcgtgccgc tcgacacacg actcccgagg gtcagcaaat caagggattg caggtgggat
     1681 ccctcggggg acgaggggag cgtgcctact aactgggcag ggctttcgga agtgccttcc
     1741 agctaggcaa cgggtctcgg gctagccttc agtctctcca gaccgcgttc gcgtcctcga
     1801 cggcgaccgc gtgcttggcc ggagccatcg cctcgaccgc agaggccggc accaccgagc
     1861 gcagcgcggc ccacagctcc ttcagatccg ccgtcttcgc ggccagttgg acgccttgga
     1921 tgtgaggacg ctcgaactcg gtgaagcttc cggagcacac gatgaccttg ggtcgcgggc
     1981 ttcgccggct gagccgctgg aggaagtcca tgccatccat tctcggcatg cggaggtcca
     2041 gcacgatgac gtccggacgt acggcaccga gtaccgagag ggcctggtag cc
//
LOCUS       DATMCV010001133         4898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_265317, whole genome shotgun sequence.
ACCESSION   DATMCV010001133 DATMCV010000000
VERSION     DATMCV010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265317"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2076
                     /locus_tag="VNU03_25975"
     CDS             <1..2076
                     /locus_tag="VNU03_25975"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77997.1"
                     /translation="ADWIGLVHRPIVPITHPHRPVPLQYLVADLSGEIHTYDAVRQGK
                     VRLLGEEREGGYNDRGRWYTRRVVDPTVMLDELESRGWLPAIYFIFSRAGCERAMETV
                     LAEGKPLLSREQRRQVDEAIREQIGENPTIAESPLNQTIFQALGMGVGLHHAGILPTV
                     KRFTELLFERGLCKVVFATETMSLGIHMPAKSVVLQSLTKRTDRGFRSLTHNELTQMA
                     GRAGRRGIDPEGKCVMALDVRDGFDDMKRVVDGPPEPVVSQFKLGYGSVALLLSTGSD
                     LPTIRRIVESSFGQYQNMKRIRSLETDVASLEMAVTDGGGFEAPCGDFPRIGRYRAVR
                     SEVEVARMARGKSGRRGGSASEAEPGRLALVRRKSGAVLGLIVGVDRRRHRNLIQVML
                     PHGGTVAMKEGNVKRVFWQTPPLHVPRDWEQRTRGLTEQMDRLSVPELIERERSHGPE
                     GALAAIECHRCPWGSQNRCEQAWKGLEGVERQLAQKREMLDAFRNAYWQEFLRVVEVL
                     ERFGAVQDAKLLAKGQLIAGLRHDNELLVAEVVDRGILADTTLAEAATLCSGLIEESR
                     SGDAAVARLFLKKRPKLKRKLQEMESVASTVLEVQRRHQLPMPVSVSTGFMPSIFRWA
                     SGEDDWGAIVEESFGGHEGDLIRAMRRLIDVLRQLAESPEVEPALAGLLARAARVIDR
                     GIVLESALI"
     gene            2112..2624
                     /locus_tag="VNU03_25980"
     CDS             2112..2624
                     /locus_tag="VNU03_25980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77998.1"
                     /translation="MRWLTRSSGLAVAALVAAACSSLTDTTLERTTRGPNAEEMFIQQ
                     SFASNGRAPSFDEKRVWQDQMDEKVFKYLREHPEMEATSRYSEFRFWRQVAPGSTPGE
                     VKVLLGEPREQTIDPALMGSLGEQHWVAIRATAKEAWVYPLGWVVYFDDKGGVVDMLR
                     KVSPLDVRDE"
     gene            2629..3279
                     /locus_tag="VNU03_25985"
     CDS             2629..3279
                     /locus_tag="VNU03_25985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWP77999.1"
                     /translation="MLDLGVFTTPMASTALTWIEPGASATLIWHGAVATGPGGGRLYA
                     ISGPALEQPPASPHFLLAPAEDDAFAARLYRGQVSLGDLRAFLARCRIAQGAMVDEMQ
                     YLAVADEAPVLPLLDGWRDAPDVPVLPYLDDINAFMPAAPLYVTAEAHAAAQREPEQF
                     STAWVCDECGDAQDAGVFFWTAQRGPAVRVCFLIHNDAGTWTCRLHPFEFAKETPA"
     gene            3276..>4898
                     /gene="ggt"
                     /locus_tag="VNU03_25990"
     CDS             3276..>4898
                     /gene="ggt"
                     /locus_tag="VNU03_25990"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWP78000.1"
                     /translation="MIRSTLSVTKQTPSTARALVAAEHPLGAEVGAGILARGGNAVDA
                     AVATAFAMTVVEPFMSSVAGGGTMLVHLAKRGETVALDFNVQAPAACHEGCFELGDGI
                     AQALFPWRQVVGDANNFGPKSVAIPGSVAGLTLALERFGTMDLADVIAPAIRLAEEGF
                     PVDWYVTLTTAPYCQELAAFPETARTYLRGGHYVHRPASLAPADVLRQPDLGRSLRLI
                     AKDGPAAFYRGAIAQAIDHEMKRTDGFLRASDLAGYEPRVVEPLTVKYRGLELAFSPG
                     ATGGPTAVEMLNVLAQFPAAKVGYETPAGLHLRAEALRYAFLDRLRHLADPLAVRVPW
                     RGLASREYAAEVAAGLRVAGPRSEAKLPDAWAHDVAVGGLGRGKAAAPRVRRRVPTEG
                     ADCTTHICSVDRQRNMVSLTNTAVSLYGSRMVVPGTGILLQNGMLWFDPEPGRANSVA
                     PGKRPVVNMVPALGFRQGEPYLTVGAPGGRKIVSAIPQVIANVADAGDTPQQAIEAPR
                     VHTEGGELWVDDRVGEKSLAALRRMGHDVVPKH"
BASE COUNT          678 a         1762 c         1789 g          669 t
ORIGIN      
        1 gcggactgga tcggcctcgt gcaccggccc atcgtgccga tcacccaccc gcatcgaccg
       61 gtgccgctgc agtacctggt cgccgacctc tcgggagaga tccacaccta cgacgcggtc
      121 cgccagggca aggtgcgcct cctcggcgag gagcgcgagg gcggctacaa cgaccgcggg
      181 cgctggtaca cgcggcgggt ggtggatccc accgtgatgc tcgacgagct ggagagccgc
      241 gggtggctgc ccgcgatcta tttcatcttc agccgcgcgg gctgcgagcg ggccatggag
      301 acggtcttgg ccgaaggcaa gcccctgctc tcgcgcgagc agcgccgcca ggtggacgag
      361 gccatccgcg agcagatcgg ggagaacccg accatcgcgg agtcgccgct caaccagacg
      421 atcttccagg ccctcggcat gggcgtcggg ctccaccacg cggggatcct gcccaccgtg
      481 aagcgtttca ccgagctcct cttcgagcgt gggctctgca aggtggtctt cgccaccgag
      541 accatgtcgc tcggcatcca catgccggcc aagagcgtgg tgctgcagtc gttgaccaag
      601 cgcaccgacc gcggcttccg cagcctcacc cacaacgagc tcacccagat ggcggggcgg
      661 gcgggccggc ggggcatcga ccccgagggc aagtgcgtga tggccctcga cgtgcgcgac
      721 ggcttcgacg acatgaagcg ggtggtggac gggccgcccg agccggtggt gagccagttc
      781 aagctcggct acggctcggt cgcgctgctg ctgtcgaccg gcagtgacct gcccaccatc
      841 cgccggatcg tcgagtcgtc gttcggccag taccagaaca tgaagcgcat ccggagcctc
      901 gagaccgacg tggcgagcct cgagatggcg gtgaccgacg gcggcggctt cgaggcgccc
      961 tgcggcgact tcccccgcat cggccgctac cgggcggtgc gctccgaagt ggaggtggcc
     1021 cgcatggcgc gcggcaagag cgggcgccgg ggcggctccg cgtcggaggc cgagcccggc
     1081 cggctggccc tggtgcgccg caagagcggg gcggtcctgg gtctgatcgt cggagtcgat
     1141 cggcgccggc accgcaacct gatccaggtc atgctgcccc acggcgggac ggtcgcgatg
     1201 aaggagggca acgtcaagcg ggtgttctgg cagaccccgc cgctgcacgt gccgcgcgat
     1261 tgggagcagc gcacccgcgg gctcaccgag cagatggatc ggctctcggt gcccgagctg
     1321 atcgagcgcg agcggagcca cgggccggag ggcgcgctcg cggccatcga gtgccaccgc
     1381 tgcccgtggg gctcgcagaa ccgctgcgag caggcctgga aggggctcga gggtgtcgag
     1441 cggcagctcg cccagaagcg ggagatgctc gacgccttcc gcaacgcgta ctggcaggaa
     1501 tttctccggg tggtcgaggt gctcgagcgg ttcggcgcgg tgcaggacgc caagctcctg
     1561 gccaagggcc agctcatcgc ggggctgcgg cacgacaacg agctgctggt ggccgaggtg
     1621 gtggaccgcg ggatcctcgc cgacaccacg ctcgcggagg cggccaccct ctgctccggg
     1681 ctgatcgagg agtcgcgctc gggggacgcg gcggtggccc ggctcttcct caagaagcgc
     1741 cccaagctca agcgcaagct ccaggagatg gagtcggtcg ccagcaccgt gctcgaggtc
     1801 cagcgccgcc accagctccc aatgccggtc tcggtctcga ccggcttcat gccgtcgatc
     1861 ttccgctggg cctccgggga ggacgactgg ggcgccatcg tggaggagtc gttcgggggc
     1921 cacgagggcg acctgatccg ggccatgcgc cggctgatcg acgtgctgcg tcagctggcg
     1981 gagtcgcccg aggtcgagcc ggccctggcc ggcctcctcg cccgggccgc ccgggtcatc
     2041 gaccggggga tcgtgctgga gtccgctctc atttgagggg cgggcggaag cgcggcgata
     2101 taatggtcgc catgcggtgg ctgacccgat cctcgggcct ggcggtggcg gcgctcgttg
     2161 cggcggcgtg ctcgtcgctg accgacacca cgctcgagcg caccacccgg ggcccgaacg
     2221 ccgaggagat gttcatccag cagtccttcg cgtccaacgg ccgcgcgccc tccttcgacg
     2281 agaagcgggt gtggcaggac cagatggacg agaaggtctt caagtacctg cgcgagcacc
     2341 cggagatgga ggcgacgtcg cgctacagcg agttccgctt ctggcgccag gtcgcgccgg
     2401 gcagcacccc gggcgaggtg aaggtgctgc tgggcgagcc gcgggagcag accatcgacc
     2461 cggccctgat gggctcgctg ggcgagcagc actgggtggc gatccgcgcc accgcgaagg
     2521 aagcctgggt ctacccgctc ggctgggtgg tctacttcga cgacaagggc ggggtggtcg
     2581 acatgctccg caaggtctcc ccgctcgacg ttcgcgacga atagccgcgt gctagacttg
     2641 ggcgtcttca cgacgcccat ggcgagtacc gcgctgacgt ggatcgagcc gggagcgagc
     2701 gccaccctga tctggcacgg cgcggtcgcc accggacccg gcggaggccg gctctacgcg
     2761 atctccggcc ccgcgctcga gcagcccccc gcctcgcccc acttcctcct ggcccccgcg
     2821 gaggacgacg ccttcgcggc ccggctctac cgcgggcagg tgagcctcgg cgatctccgc
     2881 gccttcctcg cccgctgccg catcgcgcag ggcgccatgg tcgacgagat gcagtacctg
     2941 gcggtggccg acgaggcgcc ggtgctgccg ctcctggacg gctggcgcga cgcgcccgac
     3001 gtgccggtgc tgccctatct ggacgacatc aacgcgttca tgcccgccgc gccgctctac
     3061 gtgaccgcgg aggcccacgc ggcggcccag cgggagcccg agcagttcag caccgcgtgg
     3121 gtctgcgacg agtgcggcga cgcccaggac gcgggcgtct tcttctggac cgcgcagcgg
     3181 ggccccgcgg tgcgcgtgtg cttcctgatc cacaacgacg ccgggacgtg gacgtgccgg
     3241 ctccacccgt tcgagttcgc gaaggagacg cccgcatgat ccgctccacc ctctccgtga
     3301 ccaagcagac gccgtccacc gcccgggccc tcgtcgcggc cgagcacccg ctcggggccg
     3361 aggtgggggc ggggatcctc gcgcgcggcg gcaacgcggt ggacgcggcg gtggccaccg
     3421 ccttcgcgat gacggtcgtc gagccgttca tgtcctcggt ggcgggcggc ggcaccatgc
     3481 tcgtgcacct ggccaagcgc ggcgagacgg tggcgctcga cttcaacgtg caggcgcccg
     3541 cggcctgtca cgagggctgc ttcgagctgg gcgacggcat cgcccaggcg ctcttcccgt
     3601 ggcgccaggt ggtgggcgac gccaacaact tcgggccgaa gtcggtggcc attcccggct
     3661 cggtggccgg cctcaccctg gccctcgagc gcttcggcac catggatctg gccgacgtga
     3721 tcgcgcccgc gatccggctc gccgaggagg gcttcccggt cgactggtac gtgaccctga
     3781 ccaccgcgcc ctactgccag gagctggccg cgttcccgga gaccgcccgc acctacctgc
     3841 ggggcggcca ctacgtccac cggcccgcct cgctcgcccc cgccgacgtg ctgcgccagc
     3901 ccgatctcgg ccggagccta cgcctcatcg ccaaggacgg gccggccgcg ttctaccggg
     3961 gcgcgatcgc gcaggccatc gaccacgaga tgaagcgcac cgacggcttc ctgcgcgcga
     4021 gtgacctcgc cggctacgag ccgcgcgtgg tcgagccgct caccgtgaag taccgggggc
     4081 tcgagctggc gttctcgccc ggcgccaccg gcgggccgac cgcggtcgag atgctgaacg
     4141 tcctcgccca gttcccggcc gccaaggtcg gctacgagac gccggccggc ctgcacctcc
     4201 gggccgaggc gctgcgctac gcgttcctcg accgcctgcg ccacctggcc gacccgctcg
     4261 cggtgcgcgt gccgtggcgc gggctcgcct cgcgcgagta cgccgccgag gtcgcggcgg
     4321 ggctgcgcgt cgcggggccg cgcagcgagg ccaagctgcc cgacgcgtgg gcgcacgacg
     4381 tggcggtggg cgggctcggc cgcggcaagg ccgccgcccc gcgcgtccgt cgccgcgtgc
     4441 ccaccgaggg ggccgactgc acgacccaca tctgctcggt cgaccgccag cgcaacatgg
     4501 tctcgctcac caacaccgcg gtctcgctgt acgggtcgcg catggtggtg ccgggcaccg
     4561 gcatcctgtt gcagaacggc atgctctggt tcgatcccga gcccgggcgc gccaactcgg
     4621 tggcgccggg caagcgcccg gtggtcaaca tggtgccggc gctcggcttc cggcagggcg
     4681 agccgtacct gaccgtcggg gcgccgggcg gccggaagat cgtgtccgcc atcccgcagg
     4741 tgatcgccaa cgtggccgac gcgggcgaca cgccccagca ggcgatcgag gccccgcgcg
     4801 tgcacaccga gggcggcgag ctgtgggtgg acgaccgcgt cggagagaaa tcactcgccg
     4861 cgctccggcg aatgggccac gacgtggtgc cgaagcac
//
LOCUS       DATMCV010001134         2509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_265625, whole genome shotgun sequence.
ACCESSION   DATMCV010001134 DATMCV010000000
VERSION     DATMCV010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265625"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VNU03_25995"
     CDS             complement(<1..727)
                     /locus_tag="VNU03_25995"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP78001.1"
                     /translation="MGFGSGPSLGAVLLGEGTPGHVVERARAAERGGLDSLWFIEDYF
                     QTGAFAVAGAAAAVTTRLGIGLGVVNPYTRHPALLAMETATLAGIAPGRVVLGLGAGV
                     RRWIEEQMAIPAPRPLATLAECVDVVRRLWAGERVSHEGAAFSLRDVALEFKPAQATL
                     PIVLGVKGPRALALAGTVADGVVCSIMSSPAHVRRVCDTAAAARRGAGLTGRFPVLAY
                     VPTAIGRDAEAARRAVRPLLARYL"
     gene            complement(705..1961)
                     /locus_tag="VNU03_26000"
     CDS             complement(705..1961)
                     /locus_tag="VNU03_26000"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWP78002.1"
                     /translation="MAELECLRCHAVYDDQRLFTGCPRCAEQSVNVNLTVRVPAAARG
                     PLTPESFPAAPRSLWRFRRLLPLPDAEPVTLGEGATPLVPLERLGRRLGVPHLYAKDE
                     SQNPTWSYKDRLCSLAVTHAVATGARVITIASTGNHGASTAAYAARAGLPCVIFTLAS
                     VPDTMKTLMQSYGAAVVACATSEQRWTLMREGIERCGWYPTSGWVVPPIGSNPFGVEG
                     YKTIAYEIAEDLGWTAPDVVAVPSCYSDGLFGIWKGWVELERLGLVKTRPRMLAAEPF
                     GPLAHALERKLETPEKVVAGSSVAFSIASPYGTYQGLTALRDSEGLGVPITDEGIFEA
                     QRALASEEGLYAEPSAVVGLTAVMQQVRARNLRPDQRIVVVLTSGGLKDYGASRAWLP
                     DVPAAGRDFASTLTVLEERYGLRLRP"
     gene            complement(2013..>2509)
                     /locus_tag="VNU03_26005"
     CDS             complement(2013..>2509)
                     /locus_tag="VNU03_26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWP78003.1"
                     /translation="TEPGLEATRFYDPIRGTFAAGAQAAVVEVDVATGRVHIRRYVCV
                     EDTGRVINPLIVEGQVQGALAQGIGGALLEHLIYDDAGQLLTGTLMEYALPTAADVPA
                     FELTHVEVPAENLLGVRGVGEGGTLGPPAVLANAVSDALAPFGTEVNELPITPARLWA
                     SGLG"
BASE COUNT          356 a          935 c          919 g          299 t
ORIGIN      
        1 ccaggtaccg cgcgagcagc ggccggaccg cacggcgcgc cgcctccgcg tcacgcccga
       61 tcgcggtcgg cacgtaggcg agcaccggaa accgccccgt gaggccggcc cctcgccgcg
      121 ccgcggccgc agtgtcgcac acgcggcgga cgtgggcggg cgacgacatg atcgagcaca
      181 ccaccccgtc ggcgaccgtg cccgcgaggg cgagcgcccg cggccccttg acgcccagca
      241 cgatcggcag ggtggcctgc gccggcttga actcgagcgc gacgtctcgc agagagaacg
      301 cggcgccctc gtggctcacc cgctcaccgg cccagagccg gcgcacgacg tcgacgcact
      361 cggcgagcgt ggcgagaggc cgcggcgccg ggatcgccat ctgctcctcg atccaccgcc
      421 ggaccccggc gccgagcccg agcacgacgc gtcccggcgc gatgccggcc agggtggccg
      481 tctccatggc gagcagcgcc gggtgccggg tgtacggatt gaccacgccc aggccgatcc
      541 cgagccgggt ggtcaccgcg gcggcggccc ccgccaccgc gaaggcgcca gtctggaagt
      601 agtcctcgat gaaccagagg ctgtcgaggc cgccccgctc ggcggcccgg gcccgctcga
      661 cgacgtggcc gggcgtcccc tcgcccagca gcaccgcgcc caggctaggg ccggagccga
      721 agcccatagc gctcctcgag cacggtcagc gtggacgcga agtcccggcc cgcggcgggc
      781 acgtccggca gccaggcccg gctcgctccg taatccttga gaccgcccga ggtgagcacg
      841 acgacgatac gttgatcggg gcgcaggttg cgcgcgcgga cctgctgcat caccgcggtc
      901 agcccgacca ccgcggaggg ctcggcatag agcccctcct cgctcgcgag cgcccgctgg
      961 gcctcgaaga tgccctcgtc ggtgatcggc acgccgagcc cctccgagtc gcgcagcgcg
     1021 gtgaggccct ggtaggtccc gtacggcgag gcgatcgaga acgcgaccga ggagcccgcc
     1081 accaccttct ccggcgtctc gagcttccgc tcgagcgcgt gcgcgagcgg cccgaacggc
     1141 tccgccgcga gcatgcgggg ccgcgtcttc acgagcccca ggcgctcgag ctccacccag
     1201 cccttccaga tcccgaagag gccgtcgctg tagcagctcg gcaccgccac cacgtcgggc
     1261 gcggtccagc cgaggtcctc cgcgatctcg taggcgatgg tcttgtagcc ctccaccccg
     1321 aacgggttcg agccgatcgg cggcaccacc cagccgctgg tgggatacca gccgcagcgc
     1381 tcgatgccct cgcgcatcag cgtccagcgc tgctcggagg tggcgcacgc caccaccgcc
     1441 gcgccgtagg actgcatcag cgtcttcatg gtgtcgggca ccgaggcgag cgtgaagatg
     1501 acgcacggca ggccggcccg cgccgcgtag gccgcggtgg acgcgccgtg gttgccggtc
     1561 gacgcgatcg tgatcacccg ggccccggtg gccaccgcgt gggtcaccgc gagcgagcag
     1621 aggcggtcct tgtaggacca cgtcgggttc tggctctcgt ccttggcgta gaggtgcggg
     1681 acgccgaggc ggcgtccgag ccgctcgagc ggcaccagcg gggtggcgcc ctcgccgagg
     1741 gtgaccggct ccgcgtcggg cagcgggagc agccggcgga agcgccagag gctgcgcggc
     1801 gcggccggga agctctccgg ggtcagcggg ccgcgcgcgg cggcggggac gcggacggtg
     1861 aggttgacgt tgaccgactg ctcggcgcag cgagggcacc cggtgaatag ccgctgatca
     1921 tcgtagaccg cgtggcagcg aagacactcg agctcggcca ctacgcgggc ggacgccatc
     1981 ggcggcgagg ttagcaagcg tgctgcgggg tgtcaaccga ggccgctcgc ccagagccgg
     2041 gcgggcgtga tcggtagctc gttcacctcg gtgccgaacg gcgcgagggc gtcggacacc
     2101 gcgttggcca gcaccgcggg cggcccgagg gtgccgccct cacccacccc acgcactccc
     2161 agcaggttct ccgcgggcac ctccacgtga gtcagctcga aggcgggcac gtccgccgcg
     2221 gtgggcagcg cgtactccat caacgtgccg gtcaggagct gtccggcgtc gtcgtagatc
     2281 aagtgctcga gcagcgcgcc gccgatgccc tgggcgagcg caccctggac ctgtccctcg
     2341 acgatgagcg ggttaatgac ccgcccggtg tcctcgacgc agacgtagcg gcggatgtga
     2401 acgcggccgg tcgccacgtc gacctcgacc accgcggcct gggccccggc cgcgaaggtg
     2461 ccgcggatcg gatcgtagaa gcgcgtggcc tcgaggccgg gctcggtct
//
LOCUS       DATMCV010001135         7523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_64132, whole genome shotgun sequence.
ACCESSION   DATMCV010001135 DATMCV010000000
VERSION     DATMCV010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64132"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..1990)
                     /gene="cimA"
                     /locus_tag="VNU03_26010"
     CDS             complement(386..1990)
                     /gene="cimA"
                     /locus_tag="VNU03_26010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HWP78004.1"
                     /translation="MVVTPGSRQIKIYDTTLRDGTQGEGVAFSMEDKVRIAQRLDALG
                     VHYIEGGWPGSNPKDLRFFKRVQDAVFKSAKISAFGATRRPGVRPQDDGNLQALVEAG
                     PPVVTIFGKAWDFHVTAALGTTPEENVAMIADSVAYLLKHFEEVIYDAEHFFDGFKRN
                     PDYAMRTLLAAEAAGAHCLVLCDTNGGSLPHEVAAIIRTAKRALKPETPLGIHVHNDT
                     ECAVATSLAAVGEGIGHVQGTINGYGERCGNANLVSIIPNLMLKMGLDCIPRANLREL
                     RDVSRLVSELANRKPWSSQPYVGDSAFAHKGGIHVSAVLKHPETYEHLDPETVGNQRR
                     VLVSELAGQSNVLWKAKEYGIDLDKNTPEARRILEMLKRLEDEGFLFEGAEASFELLM
                     ERALGNHRPYFELGGYRVIVEENAGDGEPVAEATVRLRVKGIEEHTAAAGNGPVHALD
                     HALRKALGDFYPNLREMALLDYKVRILDESKATAARTRVLITSGDGNDTWGTVGVADN
                     IIEASWQALVDSVEYKLRRDERQGKR"
     gene            2044..2775
                     /locus_tag="VNU03_26015"
     CDS             2044..2775
                     /locus_tag="VNU03_26015"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWP78005.1"
                     /translation="MISTVMAAAVLVAAFIKGAIGFGFPSLATPLLSLVVDVKTAVVL
                     LILPNIVMDGIQFARRGAPLATVRRFGGLLLAGGAGIVLGTRVLTLLSPRAATLILGL
                     FVLIFVTLSATGLAPKIPPRWEPWLSPPAGFVAGVIGGVTNSPATALVLYFQGIGLAK
                     HDFISSIAFTFVFYKLVQLGAVTWYGLLPWSLLWASMALTVAALAGFAVGLRVQDRLD
                     QRTFNGAVLVFLAVLGAWLLFRSLG"
     gene            complement(2791..3153)
                     /locus_tag="VNU03_26020"
     CDS             complement(2791..3153)
                     /locus_tag="VNU03_26020"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWP78006.1"
                     /translation="MTAREVELYAQITGDRNPLHFDADFAARTRFGRLVAQGGIAAGM
                     LNALVAMELPGPGTVFMSQSLKYLAPTYLGDTVTAEIEVLALKPDKPVCQLRATITNQ
                     DGTVLLEGECWTYTMRPA"
     gene            complement(3224..4483)
                     /locus_tag="VNU03_26025"
     CDS             complement(3224..4483)
                     /locus_tag="VNU03_26025"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWP78007.1"
                     /translation="MATTRARSRKTAPRRATKRAAPRRARPPLAGVRVIDLTRVLAGP
                     FCAMSLGDMGAEVIKIEEPGKGDDTRGWPPFVHGEATYFLSVNRNKKSLTLNMKAPEG
                     QEILRRLLASADVVLENFRPGTMERLGFGYAALRRANPRLVYCSISGFGESGPEAHRP
                     GYDLIVQGESGVMDITGFPDGPPVKVGNSIGDLVAGMAAAQGITLALLSREKTGKGQK
                     VEIGMLDVMASLLTYQAGLYWNGGGRPARRGNEHPSIVPYEVFKAKDVYLTLGVANNS
                     LWDRTCRALDREDLIRDPRFDTEANRVTNRKVLVPLLNEILGARPADEWLARLDKAGV
                     PAGRIKSVAEVCESAHLRARGMMVSLPHPKAGPITVMGVPVRLHDTPGAASAPPPLLG
                     QHTDEILTRLLRMPKARVDKLRAGGVV"
     gene            complement(4470..4898)
                     /locus_tag="VNU03_26030"
     CDS             complement(4470..4898)
                     /locus_tag="VNU03_26030"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWP78008.1"
                     /translation="MPDLLRASRCPRCRRLVVPPASLCPDHPVKMEAATLPPLGEVVS
                     FTTLHSPPEGFRAPLHLALVQLEGGARLFCHGAATDGVKIGSHVSVEAVDSVFYFSNM
                     GFTDRARLFWRRAGARGERIGAIGKSVVKGIWNRRPRGDD"
     gene            complement(4898..6025)
                     /locus_tag="VNU03_26035"
     CDS             complement(4898..6025)
                     /locus_tag="VNU03_26035"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWP78009.1"
                     /translation="MTDVWIAGAGMTRFGRRDENLPDLMAEAALAALADAGIERPEAL
                     VVAAMNPEEFTGEGNYASLIATRLGLSQVPALRVETATSSGVAAVYSAYAAIAAGFHR
                     SILVVGGEKMTHVATPRVSEIIGRSIDPHEKSYGTTMPALAGLVTRAAMHRRGFTLRE
                     ISQVAVKNHAHGARNPYAHFQEPVKLEAVMESRLVADPLRLFHCCPISDGGAALVLTV
                     EPRAVRIAGIGQGGDSIAVRYRSDLTSFRATQAAAQAAYRMAGFGAERVEVAELHDAF
                     TPFELISLEDTGLVPPGKAGRATLDGETSVGGRLPINPSGGLKARGHPLAATGISQIV
                     ELCWQLRGKAEGRQVPARVGLAQSIGGLATNNWVTLVEVRR"
     gene            complement(6057..6386)
                     /locus_tag="VNU03_26040"
     CDS             complement(6057..6386)
                     /locus_tag="VNU03_26040"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWP78010.1"
                     /translation="MTTLLMPAPEPAPRPSLPRLPKGLSTVEVPPAAPARPLPGFHGA
                     VSDFKRRLLEATLTQAGGNRTRAARALGLQRTYLLRLIREFEIRVPPPSVPPRRGEAG
                     AVLPQTR"
     gene            complement(6621..6854)
                     /gene="fdxA"
                     /locus_tag="VNU03_26045"
                     /pseudo
     CDS             complement(6621..6854)
                     /gene="fdxA"
                     /locus_tag="VNU03_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(6953..>7523)
                     /locus_tag="VNU03_26050"
     CDS             complement(6953..>7523)
                     /locus_tag="VNU03_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWP78011.1"
                     /translation="TAGAVLGVGSAYFGGRVDLYLQRLMDIFLSFPLIILALALVAIL
                     GNSIPNVIMAITIPMIPRAALVIRSSALTIREMPYVDAARAAGFGHGRIILRHMLPNV
                     VAPYLIMLTAFLSQAILAESSLSFLGLGVQEPTAAWGLMLRGAAVDFAEAAPWMALFP
                     GFAISLAVFAFNLFGDSLRDALDPKLRTL"
BASE COUNT         1067 a         2622 c         2670 g         1164 t
ORIGIN      
        1 agttccccag catctgctcc aacacgaacc gcgccagccg attcgattca cggtctcgcg
       61 ccagccgcac cccatcccgg tagtccgtcg gaatatcgat cgccgcccgc gcggtcaact
      121 cccgcgcagt gtcctcgatc agcttcgccg aaatactacc cacgcctcac ctctggacag
      181 accaggtcgc cgacaaaccg acgatacccg tgaacgagca gatggacctg cgctcagtca
      241 tgcgagacat cgtacgccgt gaagttagca gttaatactc ccgaagcggt agggaggggg
      301 aggggcgggg gcggcgggtg gcgtgcccgg tgagtggggt atcgaacgag ggcgcgctgc
      361 ccgccgcccc cgcccctccc cctccctacc gcttaccctg cctttcatcg cgccgcagct
      421 tgtactcgac cgaatcgacg agcgcctgcc aagaagcctc gatgatgttg tccgccacgc
      481 cgacggtccc ccacgtgtca ttcccatcgc ccgaggtgat caagacccgg gtgcgcgccg
      541 cggtcgcctt cgactcgtcg aggatgcgca ccttgtagtc gagcagcgcc atctcgcgca
      601 ggttcggata gaaatccccg agcgccttcc gcagcgcgtg gtcgagcgcg tgcaccgggc
      661 cgttgccggc cgccgcggtg tgctcctcga tacccttcac gcgcagccgc acggtcgcct
      721 cggccaccgg ctcgccgtcg cccgcgttct cctccacgat cacccggtag ccgcccagct
      781 cgaaataggg ccggtggttg ccgagcgcgc gctccatcaa gagctcgaac gaggcctcgg
      841 ccccctcgaa caggaacccc tcgtcctcga ggcgcttgag catctcgagg atgcgccgcg
      901 cctcgggcgt gttcttgtcg aggtcgatgc cgtactcctt ggccttccag agcacgttcg
      961 actgcccggc cagctcggac accagcaccc ggcgctggtt gcccaccgtt tccggatcca
     1021 ggtgctcgta ggtctcggga tgcttgagca ccgcggagac gtggatgccg cccttgtggg
     1081 cgaaggcgga gtcgcccacg tagggctggg aggaccacgg cttgcggttg gccagctcgg
     1141 agaccaggcg cgagacgtcg cgcagctcgc gcaggttcgc gcgcgggatg cagtcgagcc
     1201 ccatcttgag catgaggttc gggatgatcg agaccaggtt ggcgttgccg cagcgctcgc
     1261 cgtagccgtt gatcgtgccc tgcacgtgcc cgatgccctc gccgaccgcg gccagcgagg
     1321 tggccaccgc gcactcggtg tcgttgtgca cgtggatgcc gagcggggtc tccggcttca
     1381 gcgcgcgctt ggccgtccgg atgatcgcgg cgacctcgtg cgggagggac ccgccgttgg
     1441 tgtcgcagag cacgaggcag tgggcgcccg ccgcctccgc ggccaggagc gtccgcatcg
     1501 cgtagtcggg gttgcgcttg aagccgtcga agaagtgctc cgcgtcgtag atgacctcct
     1561 cgaagtgttt gaggaggtag gccaccgagt ccgcgatcat cgccacgttc tcctcgggcg
     1621 tggtgccgag ggccgcggtc acgtggaagt cccacgcctt gccgaagatc gtgacgacgg
     1681 gcggtcccgc ctccaccagg gcctggaggt tgccgtcgtc ctgcggccgc acccccgggc
     1741 gccgggtggc gccgaacgcg ctgatcttgg ccgacttgaa caccgcgtcc tgcacgcgct
     1801 tgaagaagcg caggtccttc gggttcgagc ccggccagcc gccctcgatg tagtgcacgc
     1861 cgagcgcgtc gaggcgctgc gcgatgcgga ccttgtcctc catggagaag gccacgccct
     1921 cgccctgggt gccgtcgcgg agggtggtgt cgtagatctt gatctggcgc gatcctggcg
     1981 tcacgaccat ggggggcaca tcatcccgct ccgccccggc gagtgtcaag ttatgctcgg
     2041 gcggtgatct cgaccgtgat ggcggcggca gtgctggtgg cggccttcat caagggcgcc
     2101 atcggcttcg gatttccctc cctcgccact cccctgctga gcctcgtcgt ggacgtgaag
     2161 accgcagtgg tgctgctgat cctccccaac atcgtcatgg atggcatcca gttcgcgcgg
     2221 cgcggggccc cgctggccac cgtgcggcgg ttcggcggcc tgctgctcgc gggcggggcc
     2281 ggcatcgtgc tcggcacgcg ggtcctgacg cttctctccc cgcgcgcggc caccctcatc
     2341 ctggggctct tcgtcctgat cttcgtcacc ctcagcgcca ccggcctcgc cccgaagatc
     2401 ccgccgcgct gggagccctg gctctcccca cccgccggct tcgtggccgg cgtcatcggc
     2461 ggggtgacca attcgcccgc caccgcgctc gtcctctact tccaggggat cgggctggcc
     2521 aagcacgact tcatctcgtc gatcgccttc acgttcgtct tctacaagct ggtgcagctc
     2581 ggcgcggtga cctggtacgg gctcctgccc tggtcgctgc tgtgggcgtc gatggcgctg
     2641 acggtggcgg cgctggccgg cttcgcggtc ggcctgcgcg tgcaggatcg gctcgatcag
     2701 cggaccttca acggcgcggt gctggtcttc ctggccgtgc tcggcgcgtg gctgctgttc
     2761 cgcagcctcg ggtgatcggc agcccggcga tcaggccggg cgcatcgtgt aggtccagca
     2821 ctccccctcg agcagcacgg tcccgtcctg attggtgatc gtggcccgca gctggcagac
     2881 cggcttgtcg ggcttgagcg cgagcacctc gatctcggcg gtgacggtgt cgcccagata
     2941 ggtcggggcc aggtacttga gcgactggct catgaagacg gtgccgggcc cgggcagttc
     3001 catggccacc agcgcgttca gcatcccggc cgcgatgccg ccctgggcca ccagccgacc
     3061 gaagcgcgtg cgcgccgcga agtcggcgtc gaagtggagc gggttgcggt cgccggtgat
     3121 ctgggcgtag agctcgactt cgcgtgcggt cacgttctgc gtccgccgcg ccttctgccc
     3181 gacctcgagc ccgagcgggc cgatcgcgcg cgccctccgc gggctacacc acgccgccgg
     3241 cgcgcagctt gtcgacgcgc gccttcggca tgcgcagcag ccgggtcagg atctcgtcgg
     3301 tgtgctgccc cagcagcggc ggcggagccg aggccgcccc cggcgtgtcg tgcaggcgca
     3361 ccggcacgcc catcaccgtg atcggccccg ccttgggatg cggcagcgag accatcatgc
     3421 cgcgggcgcg caggtgcgcg ctctcgcaga cctcggccac gctcttgatg cgcccggcgg
     3481 gcaccccggc cttgtcgagg cgcgcgagcc actcgtcggc cgggcgcgcg cccaggatct
     3541 cgttcaggag cggcaccagg accttgcgat tcgtcacccg gttggcctcg gtgtcgaagc
     3601 gcggatcgcg gatcaggtcc tcgcggtcga gggcgcggca ggtacggtcc cacagcgagt
     3661 tgttggccac gccgagcgtg aggtagacgt ccttcgcctt gaagacttcg tagggcacga
     3721 tcgacgggtg ctcgttgccc cgtcgcgcgg gacggccgcc cccgttccag tagaggccgg
     3781 cctggtaggt gagcagcgag gccatcacgt cgagcatgcc gatctcgacc ttctgcccct
     3841 tgccggtctt ctctcggctc aggagcgcca gcgtgatgcc ctgcgcggcg gccatgcccg
     3901 cgaccaggtc gccgatggag ttgcccacct tgaccggcgg cccgtcggga aagccggtga
     3961 tgtccatgac cccggactcg ccctgcacga tcaggtcgta gccgggccgg tgggcctcgg
     4021 ggccgctctc gccgaagccc gagatcgagc agtagacgag ccggggattc gcgcggcgca
     4081 gcgccgcata gccgaagccg agccgctcca tggtgccggg ccggaagttc tcgagcacca
     4141 cgtcggcgga ggcgagcagc cggcgcagga tctcctgccc ctcgggcgcc ttcatgttga
     4201 gcgtgaggct cttcttgttc cggttcacgg agaggaagta ggtggcctcg ccgtggacga
     4261 agggcggcca gccgcgcgtg tcgtccccct tgccgggctc ctcgatcttg atgacctccg
     4321 cacccatgtc gccgagcgac atcgcgcaga acggcccggc cagcacccgg gtgaggtcga
     4381 tgacgcgcac cccggcgagc ggcggccggg cgcgacgggg cgcggcccgc ttcgtggcgc
     4441 ggcgcggggc ggtcttgcgg gagcgggctc tagtcgtcgc cacggggcct cctgttccag
     4501 atacctttca cgacactctt gccgatggcg ccgatacgct cgccgcgcgc cccggcgcgc
     4561 cgccagaaga ggcgggcccg gtcggtgaat cccatgttcg agaagtagaa caccgagtcg
     4621 accgcctcca ccgagacgtg ggagccgatc ttgacgccgt cggtggccgc gccgtggcag
     4681 aagagccgcg cgccgccctc gagctgcacg agcgccaggt ggagcggggc ccggaagccc
     4741 tcgggcggcg agtgcagcgt ggtgaacgag accacctcgc cgagcggcgg cagcgtggcc
     4801 gcctccatct tcaccgggtg gtccgggcag aggctggccg gcggcaccac caggcggcgg
     4861 cagcgggggc agcgggaggc ccggagcagg tccggcatca gcggcggacc tccaccagcg
     4921 tcacccagtt gttggtggcg agcccaccga tcgactgggc cagcccgacc cgcgcgggga
     4981 cctggcggcc ctcggccttc ccgcggagct gccagcacag ctcgacgatc tgggagatgc
     5041 cggtcgcggc cagcgggtgg ccacgcgcct tcagcccccc cgacgggttg atcggcagcc
     5101 ggccgcccac ggaggtctcg ccgtcgaggg tggcgcgccc cgccttgccg ggcggcacga
     5161 ggccggtgtc ctcgagcgag atcagctcga acggggtgaa ggcgtcgtgc agctcggcca
     5221 cctcgacccg ctcggcgccg aagccggcca tgcgatacgc cgcctgcgcg gccgcctggg
     5281 tggcccggaa ggacgtgagg tcgctgcggt agcgcaccgc gatcgagtcc cctccctgcc
     5341 cgatgccggc gatccgcacc gcccggggct cgacggtcag cacgagcgcc gcgccaccgt
     5401 ccgagatcgg gcagcagtgg aagaggcgca gcgggtcggc cacgaggcgg ctctccatca
     5461 ccgcctcgag cttcaccggc tcctggaagt gggcgtaggg gttgcgcgcg ccgtgggcgt
     5521 ggttcttcac cgccacctgc gagatctccc ggagcgtgaa gccgcgacgg tgcatcgcgg
     5581 cccgggtgac caggccggcc agcgcgggca tcgtggtgcc gtagctcttc tcgtgaggat
     5641 cgatcgagcg gccgatgatc tcggacacgc gcggggtggc cacgtgggtc atcttctcgc
     5701 cgcccaccac caggatcgag cgatgaaagc cagccgcgat cgcggcgtag gcggagtaaa
     5761 ccgcggcgac ccctgaggac gtggcggtct ccacccggag ggccggcacc tgcgagaggc
     5821 cgagccgcgt ggcgatcagc gaggcgtagt tgccctcccc ggtgaactcc tcggggttca
     5881 tggccgccac cacgagcgcc tcgggcctct cgatgcccgc gtcggccagg gcggccagag
     5941 cggcctcggc catgaggtcg gggagattct cgtcgcgccg tccgaagcgg gtcatgcccg
     6001 ctccggcgat ccacacgtcg gtcacggacc catttcaacc gggccgcgcg cgggtgtcaa
     6061 cgggtttgcg gaaggaccgc gccggcctct ccacgccgcg gcggcaccga cggcggcggg
     6121 acgcggatct cgaactcgcg aatgaggcgc aggagatagg tgcgctgcag ccccagcgcg
     6181 cgcgccgccc gcgtgcggtt gccgcccgcc tgggtgagcg tggcctcgag caggcgccgc
     6241 ttgaagtcgg agacggcgcc gtggaagccg ggcagcggac gggccggcgc ggcgggcggc
     6301 acctcgacgg tggagaggcc cttcggcagg cgcgggaggg acggccgggg agcgggctcg
     6361 ggagcgggca tgagtagtgt ggtcatgcgg cagaccatgg caaggcatgt gccacgcggg
     6421 atcggggtcg ggaccgcgaa tttggcgggg ctcatcgtgc tacttgcacc gggccgtgtg
     6481 cagggccggc cgcgccgtgc cgctgcgcgt gggcacggac acgaaaaaac cggggacccg
     6541 ccgaacggcg ggtccccggt gacggtcctc gcgggggccg gagtcgccta cgccttggtg
     6601 gccggcttga cgcccgggtt gtccttgaag aactgcttgt tcaggtcgat gaagttcttc
     6661 cagttggccg gagtctcgtc ttccgcgaag atcgccttca ccgggcagac cggctcgcaa
     6721 gcgccgcagt cgatgcactc atccgggtgg atgtagagca tggtctcgcc ctcgtagatg
     6781 caatccacgg ggcagacctc gacgcaggcc ttgtccttca cgttgatgca gggctcagcg
     6841 atgatatacg ccattctccg gcctcctgtt aaccggtctc ccgggaaaac tctctcgaaa
     6901 cccccacact tctaccaact tgcgcgacac gtgtcaaggc gtttgacccc ggctagagtg
     6961 tgcgcagctt cgggtccagg gcgtcccgga gcgagtcgcc gaagaggttg aaggcgaaca
     7021 cggccaggct gatcgcgaag ccggggaaca gcgccatcca cggggccgcc tccgcgaaat
     7081 ccaccgcggc accgcgcagc atcagacccc atgcggcggt gggctcctgc acccccaggc
     7141 ccaggaacga cagcgaggat tcggccagga tggcctggct caggaacgcg gtgagcatga
     7201 tcaggtacgg agcaaccacg ttgggcagca tgtggcgcag gatgatgcgc ccgtgcccga
     7261 agccggcggc gcgcgccgcg tccacgtagg gcatctcccg gatcgtgagc gcggaggagc
     7321 ggatgaccag cgcggcgcgc gggatcatcg ggatcgtgat ggccatgatc acgttcggga
     7381 tgctgttgcc gaggatggcg accagggcca gcgcgaggat gatcagcggg aacgacagga
     7441 agatgtccat caggcgctgg aggtacaggt cgacccgtcc gccgaaatag gcgctgccga
     7501 ctccgaggac ggccccggcg gtg
//
LOCUS       DATMCV010001136         3111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U13391 k141_65685, whole genome shotgun sequence.
ACCESSION   DATMCV010001136 DATMCV010000000
VERSION     DATMCV010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942797
            Sequence Read Archive: SRR7968838
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:34:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 26
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 4 of 25
            Pseudo Genes (incomplete)         :: 22 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 3 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65685"
                     /isolate="SMAG_U13391"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="44.23 N 122.22 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VNU03_26055"
     CDS             <1..855
                     /locus_tag="VNU03_26055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HWP78012.1"
                     /translation="DSIMVAGKYQTRPPFPFSPGLETAGVVAACGARVTRFRPGDRVM
                     ATLGWGGLAELAVAKESETFAIPEGMAFEEAGAFPIAYISSDVAIRWQGRLEAGETLL
                     VLGAAGGVGLTAVEIGKAMGARVIAGASTAEKLAVARAHGADDLVNYATENLTERVMA
                     LTGEKGADVCFDPIGGELADAALSALGWGGRMLLVGFVGGVQQIPANRLLVKNRSALG
                     CSLRYYRNQAPDKLRRSVEALLAWYREGRLKPLVSHRLPLERTVEAIKLLTDRKALGK
                     IVVVPTGA"
     gene            860..1615
                     /locus_tag="VNU03_26060"
     CDS             860..1615
                     /locus_tag="VNU03_26060"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWP78013.1"
                     /translation="MEIRTEAHGPHVVVVTVDNLPRLNAMTRAMMADLARVWDELERG
                     PCRAIVLTGAGTRAFCAGADISGDLSAPTDAARVISHALLKYDSYAKPIVAAVNGDCV
                     GGGLELLLATDVRAAVPTARFGLPEVKWSIYPFGGATVKLIPQIGYVHAMDLLLTGRL
                     IDAEEAARIGLINRVVPADGLMAWALETAEAIAANSPSAVQAVKRQISATIAEHARSR
                     EALDQELGDRVRASAHFKEGVAAFREKRRPRYE"
     gene            1623..2672
                     /locus_tag="VNU03_26065"
     CDS             1623..2672
                     /locus_tag="VNU03_26065"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWP78014.1"
                     /translation="MTAPVRVHIRVPATAPMPDLMRLIQGIEAAGFDGAGILDSQLLS
                     RDAFVTLGQAATHTKRLALFPAVTNPFTRHPSVLASAIQTVEELAPERVKFVIGTGYT
                     SASTIGRKAATLGEMRECIATVRTLLAGESARFGETAGRLGYASGRRIPVLMAASGPK
                     AIELAGEVADGALLLVGANRGIVTRALEHLDRGAKRAGRRVEDLEVIWAVRTATLPTT
                     AAALREARPTAVHWGILRWGGYWLEPAGLRLPPLTVPDAVWKVYPDLSHAHDWEAAIA
                     ATSFVPDEVVAQLCEAMGLVGTPADCAARIAELTRLGVRNLYLMPLLTFAPPEREIAA
                     FRDTVFPTLARAGLR"
     gene            complement(2669..>3111)
                     /locus_tag="VNU03_26070"
     CDS             complement(2669..>3111)
                     /locus_tag="VNU03_26070"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HWP78015.1"
                     /translation="GYYQDPERNAASFNAEGFLLSGDLGFVGADGRLRFRGRIKEMVK
                     TGGINVAPVEVEEILLGHRDVEQAYVVGIPDPRKEEILVAVVVAKVGHRIEPAALHAL
                     CKDALAAYKVPQAIHVMARDALPVTSTGKVQKFRLAEQLAAARG"
BASE COUNT          413 a         1124 c         1157 g          417 t
ORIGIN      
        1 gactccatca tggtggccgg caagtaccag acgcgcccgc cgtttccgtt cagccccggt
       61 ctcgagaccg cgggcgtggt cgcggcgtgc ggcgcgcggg tgacccgctt ccggccgggc
      121 gaccgggtga tggccacgct cggctggggt gggctcgccg agctggcggt ggccaaggag
      181 agcgagacct tcgcgatccc ggaagggatg gcgttcgagg aggccggcgc gtttcccatc
      241 gcctacatct cgagcgacgt ggcgatccgc tggcagggcc gcctggaggc aggcgagacg
      301 ctgctggtgc tgggcgcggc cgggggcgtg ggcctcaccg cggtggagat cggcaaggcg
      361 atgggcgcgc gggtgatcgc gggggccagc accgcggaga agctcgcggt ggcccgggcg
      421 cacggcgccg acgacctcgt gaactacgcc accgagaacc tcaccgagcg ggtgatggcg
      481 ctgaccgggg agaagggcgc ggacgtctgc ttcgacccga tcggcggcga gctggccgac
      541 gccgcgctct ccgcgctcgg ctggggcggc cgcatgctgc tggtgggctt cgtgggcggc
      601 gtccagcaga tcccggccaa ccggctcctc gtcaagaacc ggtcggcgct cggctgctcg
      661 ctgcgctact accgcaacca ggccccggac aagctgcgtc gctcggtcga ggcgctgctc
      721 gcctggtacc gcgaggggcg gctcaagccg ctggtcagcc accgcctgcc gctcgagcgg
      781 acggtggagg cgatcaagct cctgaccgac cgcaaggcgc tcggcaagat cgtggtcgtg
      841 cccacgggag cctgaggcca tggagatccg caccgaggcg cacgggccgc acgtggtggt
      901 ggtcaccgtc gacaacctgc cgcggctcaa cgcgatgaca cgggcgatga tggccgacct
      961 cgcccgcgtg tgggacgagc tcgagcgcgg gccgtgtcgc gccatcgtgc tcaccggcgc
     1021 gggcacgcgg gccttctgcg cgggcgccga catcagcggc gatctcagcg cccccacgga
     1081 tgccgcgcgg gtgatcagcc acgccctgct caagtacgac tcgtacgcca agccgatcgt
     1141 ggccgcggtc aacggcgact gcgtgggcgg cggcctggag ctgctgctcg ccaccgacgt
     1201 gcgcgccgcg gtgccgaccg cgcgcttcgg gctgccggag gtgaagtggt cgatctatcc
     1261 gttcggcggc gccaccgtga agctcatccc gcagatcggc tacgtgcacg cgatggacct
     1321 gctgctcacc gggcgcctga tcgacgcgga agaggcggcg cggatcggcc tgatcaaccg
     1381 cgtggtgccc gccgacggcc tgatggcgtg ggcgctcgag accgcggagg cgatcgcggc
     1441 caacagcccg tccgcggtgc aggcggtgaa gcggcagatc agcgcgacca tcgccgagca
     1501 cgcgcggagc cgcgaggcgc tcgatcagga gctgggcgac cgggtgcggg cgagcgcgca
     1561 cttcaaggaa ggcgtggccg cgttccgcga gaagcgccgg ccgcgctacg agtgaggcgt
     1621 ccatgaccgc ccccgtccgc gtccacatcc gcgtgcccgc caccgcgccc atgcccgatc
     1681 tgatgcgcct gatccagggg atcgaggcgg ccggcttcga cggcgcgggc atcctggaca
     1741 gccagctcct ctcgcgcgac gccttcgtca cgctcggcca ggcggccacc cacacgaagc
     1801 gcctcgcgct gttcccggcg gtgaccaacc cgttcacccg ccacccctcg gtgctcgcct
     1861 ccgcgatcca gacggtggag gagctggcgc cggagcgcgt gaagttcgtg atcggcaccg
     1921 gctacacctc ggcgagcacc atcgggcgca aggcggccac gctcggcgag atgcgcgagt
     1981 gcatcgccac cgtgcggacg ctgctggccg gcgagagcgc gcgcttcggc gagaccgcgg
     2041 ggcgtctcgg ctacgcgtcc ggccggcgca tcccggtgtt gatggccgcc tccgggccca
     2101 aggcgatcga gctggccggc gaggtggccg acggcgcgct gctgctggtc ggcgccaacc
     2161 gcggcatcgt cacccgcgcg ctcgagcacc tcgaccgcgg cgcgaagcgg gccgggcgcc
     2221 gggtggagga cctcgaggtc atctgggcgg tgcgcaccgc gacgctgccc accaccgcgg
     2281 ctgcgctccg cgaggcgcgg ccgaccgcgg tgcactgggg catcctgcgc tggggcgggt
     2341 actggctcga gccggccggc ctgcgcctgc cgccgctgac ggtgccggac gcggtgtgga
     2401 aggtctaccc cgatctctcg cacgcccacg actgggaggc ggccatcgcc gccacctcgt
     2461 tcgtgcccga cgaggtggtc gcccagctct gcgaggcgat ggggctggtc ggcacgccgg
     2521 cggactgcgc ggcccgcatc gcggagctca ccaggctcgg cgtgcgcaac ctctacctga
     2581 tgccgctgct gaccttcgcg ccgcccgagc gcgagatcgc cgccttccgc gacaccgtct
     2641 tcccgaccct cgcccgcgcc ggcctccgct agccgcgggc ggcggcgagc tgctccgcga
     2701 ggcggaattt ctggaccttg ccggtggagg tgaccggtag agcatcgcgc gccatcacgt
     2761 ggatcgcctg cggcaccttg taggcggcga gcgcgtcctt gcagagggcg tgcagcgcgg
     2821 cgggctcgat gcggtggccc accttcgcca ccaccaccgc gaccaggatc tcctccttgc
     2881 gcgggtcggg gatgccgacc acgtaggcct gctcgacatc acggtggccc aggaggatct
     2941 cctcgacctc caccggggcc acgttgattc ccccggtctt gaccatctcc ttgatgcggc
     3001 cgcggaagcg caggcgcccg tcggccccca cgaagccgag atcgccggac agcaggaagc
     3061 cctccgcgtt gaaggacgcg gcgttgcgct ccgggtcctg gtagtagccc g
//
